LOCUS       DAOPYB010000001        55456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_438_length_55456_cov_10.319543, whole genome shotgun sequence.
ACCESSION   DAOPYB010000001 DAOPYB010000000
VERSION     DAOPYB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 55456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55456
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_438_length_55456_cov_10.319543"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /gene="murI"
                     /locus_tag="PKH58_00005"
     CDS             complement(<1..814)
                     /gene="murI"
                     /locus_tag="PKH58_00005"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNX87435.1"
                     /translation="MTKISGPIGIFDSGYGGLTILEQIRKELPGYDYLYLGDNARTPY
                     GTRSFDVVYRFTLQCVEYLFRQGCHLVILACNTASAKALRTIQQNDLSKIDPNRRVLG
                     VIRPTVEEVAKFTKTKHIGLLATSGTVLSDSYPLEIHKIDPDIVVSSEACPLWVPLIE
                     NNEHQNEGADYFVKKYIHNLLNQDSQIDSVILGCTHYPLLYDKIARQLPEGVKIISQA
                     EIVAGSLANYLQRHPEMEEKCSRNASCRYLTTENPGKFSSSASIFLAEEIVAE"
     gene            complement(984..1952)
                     /locus_tag="PKH58_00010"
     CDS             complement(984..1952)
                     /locus_tag="PKH58_00010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX87436.1"
                     /translation="MAKKKFYFNPETLDYEEIKINLTYRLKRILFHTMSGIFVGLLFF
                     LLFVSIIKSPGEKEAALAKSRMEGQYKALQGQMSDIQGVLTDLQQRDDNLYRVVFQAE
                     PIPYEVRRTASMDAAYYDQLLRMTNSQIVVETTKKLNELRKQLYVQSKSYDEIVVLAQ
                     NKEKMLEAIPAIQPVANKDLTRVASGYGMRIDPIYHSRRFHAGMDFTAPTGTDIYATG
                     NGRVKSAGWQQGYGNCVEIDHGFGYVTLYGHMSNISVRAGQAVNRGEVIGLVGNTGKS
                     TGPHLHYEVHYKGEIMNPQNYYFLDLTPEEYDKMVQLSNNAGQMFD"
     gene            2180..4783
                     /gene="alaS"
                     /locus_tag="PKH58_00015"
     CDS             2180..4783
                     /gene="alaS"
                     /locus_tag="PKH58_00015"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNX87437.1"
                     /translation="MTSKEIRQSFLDFFESHGHKIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKNIILGNDPIKYSRVADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAITWAWEYLTEVLKLDKDRLYVTVFEGSEYEKLERDNEAAEIWSQFLPAERIINGN
                     KKDNFWEMGDTGPCGPCSEIHIDIRPDSERAKVDGLSLVNQSHPQVIEIWNNVFMQFN
                     RKADGSLEELPAKVIDTGMGFERLCMAIQGKTSNYDTDVFQPIIEEIGKLSQIKYGES
                     EKTDIAMRVVADHVRTIAFSIADGQLPSNAKAGYVIRRILRRAVRYGYTFLEQRQAFM
                     YRLMPVLCEVMGGAYPELTVQKSLIEKVMKEEEEAFLRTLETGIKLLDKVMDDAAKLN
                     KKEISGIDAFTLYDTFGFPLDLTELILREHSFTVNIEEFHAEMQKQKERARNAAAIET
                     GDWVTVREGDSVFVGYDKTETETNILRYRKVSQKNKTFYQIVLSETPLYAEMGGQVGD
                     RGTLTVNNETIEIFDTKKENNLSVHLTNSLPEDVTAPCFVRIDVAKRKATACNHTATH
                     LLHEALREVLGKHVEQKGSFVSPESLRFDFSHFQKLTNEELRAVEKIANRKIRENYPL
                     QESRNTPIAEAQAMGAMALFGEKYGETVRVIRFGSSIELCGGTHVQATGDIGMVRIIS
                     ESSIAAGVRRIEAITGEAVEELMNNVQDTLNSARELFNNAPDLYKAIHKTLEENSELK
                     KQVDNFMHERILTLRDKLIADAKDEQGIKVIRFRTDLPADAVKTLSFQIRNLLNEKLF
                     VAITSLHEGKPSITVLLSDDLVAQGYNAVNITREAAKCIQGGGGGQPFFATAGGKNPE
                     GLDKAMEMLKL"
     gene            4975..5679
                     /locus_tag="PKH58_00020"
     CDS             4975..5679
                     /locus_tag="PKH58_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNX87438.1"
                     /translation="MRILVLKSVFLIIFLVGNFSINAQDWANLKRYQKENDTLLLKGN
                     AENRVVFMGNSITEGWKSMHPEFFNKNPYICRGISGQTTPQMLLRFRQDVIELNPRVV
                     VILAGTNDIAGNTGPSTPEMIMDNIRSMAELATANNIRVVLCSVLPAYDYPWRKGMKP
                     NIKIPELNKMIKAYADERRITYLDYFGAMANEKNGMIAEYTTDGVHCTSAGYDVMEKM
                     VIPAIAKALKTKPAKK"
     gene            complement(5761..6475)
                     /gene="bla"
                     /locus_tag="PKH58_00025"
                     /pseudo
     CDS             complement(5761..6475)
                     /gene="bla"
                     /locus_tag="PKH58_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083750.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
     gene            complement(6614..8566)
                     /locus_tag="PKH58_00030"
     CDS             complement(6614..8566)
                     /locus_tag="PKH58_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX87439.1"
                     /translation="MKTPKIKLFFLLVTFLGVQLSVAQTHNIQVDFKKNGIPVQKTMY
                     GIFFEDINYGADGGLYAELVKNRSFEFPEHLMGWSTFGKVEVKTDGPFKNNPHYIRLA
                     YAGHDEKQTGLDNEGYFGIGVKKDAEYRFSVWAKKASAATGSIRVEIIDTESNVIANA
                     KLDIPSTEWKQNELILKPSKTLPDAKLRIFLTSKSEVDLEHVSLFPVDTWKGRKNGLR
                     KDLAQALYDLKPGVFRFPGGCIVEGTDLNSRYQWKNSIGQVENRMLNENRWQYTFPNR
                     FFPDYYQSYGLGFYEFFQLSEDIGAEPLPVLSCGLACQFQNKDIHAHVPVAELNPYVQ
                     DALDLIEFANGDVTTKYGKIRSEMGHPVPFNLKLIAIGNEQWGELYPERLEPFITAIR
                     ARHPEIKIIGSAGPSPDGKDFDYGWQEMKRLKADLVDEHYYRSPEWFLSGAARYDKYD
                     RKGPKVFAGEYACHPANRKNNFEAALCEAAFMTGFERNADVVQMCTYAPLFAHVQGWQ
                     WKPDLIWFDNLRSVRSVNYYVQQLYGHNTGTNVLTTTENKTALTGQDGIYASSVYDSG
                     KNEIILKIANTSDSEKSVTYHLKGLKTAERKVTKTVLKSTKPDAENTLDKPETVVPAS
                     EEINIPGSDFELKLAPKAFYLLNIKL"
     gene            complement(9010..9765)
                     /locus_tag="PKH58_00035"
     CDS             complement(9010..9765)
                     /locus_tag="PKH58_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNX87440.1"
                     /translation="MEKSLNLTLKVWRQAGPKAKGSFETYKVENISTDASFLEMMDIL
                     NEKLIGEHKEPIAFDHDCREGICGMCSMYINGHPHGPDGEITTCQLHMRNFSDGETIT
                     IEPWRSAAFPVIKDLMVDRSAYDKIIQAGGYVSINTGGTPDANAIPISKVNADEAMDA
                     ASCIGCGACAAACKNGSAMLFVSAKVSQLALLPQGRVEAAQRAKAMVAKMDELGFGNC
                     TNTGACEAECPKNVSISHIARLNREFLGAKFKD"
     gene            complement(9894..>10901)
                     /locus_tag="PKH58_00040"
     CDS             complement(9894..>10901)
                     /locus_tag="PKH58_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNX87441.1"
                     /translation="PTDLKEEDRDYYLERRYPAFGNLVPRDVASRAAKERCDAGFGVG
                     NTGLAVYLDFSEAIDRLGENVVRARYGNLFQMYEKIVDDNPYKTPMMIYPAIHYTMGG
                     IWVDYELQTSVKGLFCIGEANFSDHGANRLGASALMQGLADGYFVLPYTIQNYLADQI
                     RVPRISTDSKEFEEAEKAINDRINKIMSIQGKYSVDHLHRELGLIMWDYVGMGRTAEG
                     LKTGLEKIAQVRKDFWTNVYVPGKADNLNVELEKALRLADFIETGELMARDALLREES
                     CGGHFREEYQTPEGEALRDDDKFAYVSCWKYKGEGQEPELIKEPLDYEFIVRQQRNYK
                     A"
     assembly_gap    10902..11001
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11182..11856)
                     /locus_tag="PKH58_00045"
     CDS             complement(11182..11856)
                     /locus_tag="PKH58_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNX87442.1"
                     /translation="MWLIDSSIGRKVIMSVSGIFLILFLTFHSIMNVTVLFSGEAYNA
                     VCEFLGANWYAVVGSMVLAAGFVVHIIYALYLSILNYKARGTNRYAVTKRQEGVKWAS
                     KNMLILGGVIFGFMALHLFQFWYKMQYAELSGVHTGDFHPADGTAFIIQLFSQPLYCV
                     LYIVWLAALWFHLTHGVWSALQSIGLNNKTWLPRLKCFANIYSTFIILLFMAIPVLFL
                     FGFRPM"
     gene            12093..13517
                     /gene="hydG"
                     /locus_tag="PKH58_00050"
     CDS             12093..13517
                     /gene="hydG"
                     /locus_tag="PKH58_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNX87443.1"
                     /translation="MKFTPETYKLPDKPITPFIDTDEIEGYLNNTIPIRERVREVIAK
                     SLSKKRLNLEETAVLINATDPELIQEIKEGAKQLKKNIYGNRIVLFAPLYVGNKCSNN
                     CTYCGFRASNTAEVRKSLTDDELKLEIEALEDNGQKRLILVYGEHAQYDAEYIAHTVK
                     VAYSVKKGNGEIRRVNINAAPMEIEGFRTVKKAGIGTFQIFQETYNREAYKTYHLRGK
                     KADFDYRLTSLDRAQEAGIDDVGIGALFGLYDWRFEVMGLVRHTNHLEACYNVGPHTI
                     SFPRVKDASMLDMKDTYFVSDEDFARLVAILRLAVPYTGMILTAREPAALRNELIQYG
                     VSQIDGGTKIEIGSYVETQNTKQDLNRGQFRIGDDRSLNEVVEELLSQDMIPSFCTAC
                     YRLGRTGEHFMEFSVPGFIKRYCTPNAILTLTEYLMDYAPEKTAEKGWKAIEKNILTL
                     DETMQKQVRERIEKIKQGDRDLYF"
     gene            13587..14846
                     /gene="hydF"
                     /locus_tag="PKH58_00055"
     CDS             13587..14846
                     /gene="hydF"
                     /locus_tag="PKH58_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNX87444.1"
                     /translation="MKKKHITDKIYPTSPPSEPVPNRFREGRGAFIGIFGRRNTGKSS
                     LINLLTGQDIAIVSDKPGTTTDPVSKSVEIFGIGPVVLIDTAGIDDAGELGEKRIKKS
                     KEAIKKADCAILVIAENTFDNFELDLIKLFDEYAVPYLIVHNKSDLSPLTTETIAEIK
                     SHSSSEILDFSTQNEEKKELLISLLKSTIPTTNYQKPSLLGGLVQPKDVVLLITPIDS
                     EAPEGRMILPQNMAIRDVLENNCITVVVKETELEDFFKLGIKPALAITDSQAFNFVSK
                     IVPTDVPLTSFSIVFARLKGDFEKYLEGTPHISQLKDGDRVLILESCTHQVSCDDIGR
                     VKIPGLLQKFTGKKLEFTVVSGLSEVTENVESLSLVIQCGGCMVTRKQLLNRLKPFTE
                     AEIPVTNYGMALAYVNGIFERATAPFL"
     gene            14877..15686
                     /locus_tag="PKH58_00060"
     CDS             14877..15686
                     /locus_tag="PKH58_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87445.1"
                     /translation="MRTLFLVSLLCISSYVCSQTVEIDLFGNIQINNTINDDCDGQKK
                     VIKKDIFGNVVIEENNGKKVTVIKKDIFGNTIVEDGSGRTINVFKTDIFGNQVIEDGQ
                     GNTVRKIKKDIFGNTIIEDGHGFTTLKIEKDIFGDTIILDGSGHRIRKIKRDILGDRI
                     IENENGKTIIRLKKDILGNLIAEDETGFKIRTLKKDILNNYQIEDKYGQTLFEIKKDI
                     LGKISVRDQNNKVVKEISKDIFNNIIYQDRVCNKHNIDAYLIDFAIQQIGI"
     gene            15693..16766
                     /gene="hydE"
                     /locus_tag="PKH58_00065"
     CDS             15693..16766
                     /gene="hydE"
                     /locus_tag="PKH58_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNX87446.1"
                     /translation="MSITTLLHKSQFSKPEIVRLLSATDEDEKLLFDYASEIKQANVG
                     NTVFLRGLIELSNICEKDCYYCGIRKSNFNVKRYKISEEETVETIRYAYEKGYGSVAI
                     QSGEVQSKTFTEKINRILRKAMEISNGELGITLSCGEQTEETYRRWFESGAKRYLLRI
                     ETSSPELYRKLHPGDKKHSFEKRLEALQTLKSIGYQTGTGVMIGLPFQTVEDLADDLL
                     FMKDFDIVMCGMGPYIEHHETPLFQYHNQLLSFNERLELSLKMVAILRIMMPDINIAA
                     TTALQTIEKNARIKAIKIGANVLMPNITPHRYRDDYFLYENKPVSGQSREDDLENLDK
                     SLQAIVHRIGYGVQGNSKHFISP"
     gene            complement(16909..17388)
                     /gene="nuoE"
                     /locus_tag="PKH58_00070"
     CDS             complement(16909..17388)
                     /gene="nuoE"
                     /locus_tag="PKH58_00070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNX87447.1"
                     /translation="MNIKIRLSDEKISMINEICARFDNDPGELINVLHQTQDTFGYLP
                     AEVQELIAHNLRVSAAHVYGVVTFYSYFSMLPKGDYPISICMGTACYVRGAEKVLDEF
                     KKELNIKVGETTDDGRFSINCLRCVGACGLAPVVMVGEKVFGRVSPDKVRDILDEYK"
     gene            complement(17492..19264)
                     /locus_tag="PKH58_00075"
     CDS             complement(17492..19264)
                     /locus_tag="PKH58_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNX87448.1"
                     /translation="MENIELTIDGKILRVKEGTTILKAAAEAGIDIPTLCHFRLHDMD
                     IENKPGGCRICVVEVEGRRNLAPACITECTPGMVINTHNTRVLNARKTVLKLIVSDHP
                     FDCLVCAKSGECELQHLAQKFGIRDIPYQGEKSTYRKDTSPSIIRDVDKCIMCRRCEM
                     MCNEVQTVGALSAVNRGFMSVVAPAFEMDLVHSPCTYCGQCVSVCPTGALTEVDNTNE
                     ILRVLSDKSKTVIVQTAPAVRAALGEAFGLPPGTLVTGKMVAALRRLGFKYVFDTDFA
                     ADLTIMEEGTELLCRLKRHLNGDKTVKLPILTSCCPGWVNFFEHNFPDMLDVPSTAKS
                     PQQMFGAVAKTYFAEKIGVNRKDIVVVSVMPCLAKKYECQRDEFKVNGNPDVDFSMST
                     RELAHFIQQSNIDFMDLPSEGFDNPLGESTGAGVIFGTTGGVIEAACRTAYEIYTGKP
                     LERLEFTELRGLEGIRSATVDFDGLKLNIGIAHGLGNARKLLNEVREGKSTYHAIEIM
                     ACPGGCIGGGGQPYHHGQSDILKKRARAIYTEDERKTLRKSHENPQIVRIYDEFLGEP
                     GSPKAHELLHTHYFDKSEEVSILD"
     gene            complement(19293..21107)
                     /locus_tag="PKH58_00080"
     CDS             complement(19293..21107)
                     /locus_tag="PKH58_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNX87449.1"
                     /translation="MEKFRMQLMVCAGNSCRHHNSEDVITRIKWRIETDEELMNSVQV
                     IQTACFGLCASGTIIKVLPGNIFYANVKPEDTDEIINEHIINGRVVERLLYHPDDQKV
                     ITGTKSLNRKQIRIALRNCGVVNPENIDHCIKHNSYLAMGKVLTEYSPAETIEIIKKS
                     GLRGRGGGGFPCGLKWEIASKNDSDQKYVVCNADEGDPGAFMDRSILEGDPHAVLESM
                     AICGYCIGASKGLIYIRAEYPLAIKRLKIAIEQAKNYGLLGVDILGTGFNFDISLRYG
                     AGAFVCGEETALIHSMEGKRGEPTNKPPFPAQSGYLGKPTNVNNVETFANIPQIILNG
                     ADWFASIGTEKSKGTKVFALAGKINNVGLIEVPMGTTLREIIYEIGGGIRGGKKFKAV
                     QTGGPSGGCLTEKHLDIPIDYESLTQAGSMMGSGGMIVMDEDDCMVSVAKFYLDFIVE
                     ESCGKCAPCRIGNKRLYEMLDKITQGKGTLEDLERLKNLCNVIKDTSLCGLGQTSPNP
                     VLSTMENFAEEYYEHVVDKKCRSGHCKSLYYYVVSDELCVGCGACRRCCPVHAITGDK
                     KQIHHIDPDICIRCGECIEKCSFDAITIELEPKRVTVL"
     gene            complement(21126..21506)
                     /locus_tag="PKH58_00085"
     CDS             complement(21126..21506)
                     /locus_tag="PKH58_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNX87450.1"
                     /translation="MEKIKSYEELKALRETLRIGRPDKSSEDKPVRVKVAMATCGIAS
                     GAKEVLDYFTAELKKRSVSCSISQTGCMGYCYAEPTVEIILPNREPVVFGYVDTVRAD
                     EIIEKYIKTGELVDGIIPTNYERR"
     gene            complement(21809..23566)
                     /locus_tag="PKH58_00090"
     CDS             complement(21809..23566)
                     /locus_tag="PKH58_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNX87451.1"
                     /translation="MKITINNQPLEVQEGLTIMEACNAVGIHVPCLCYLKDVSHNASC
                     GVCVVEVKNAKSLLRSCITQVTDGMDITTNSQRVMEARRVNIELMLANHPQECLGCDR
                     NQNCELQEITFDLGIHERPFVRTKKEYSPKDESSLSLVRDPEKCILCGRCVAVCREVQ
                     GVKAIDFAWRGLKSRISTYMDLGMGIVACTNCGQCALVCPTGAITEKSAIGDVWKALN
                     DPEKVVLVQTAPAIRVGIGEAMGMPYGSLVTGQMVAGLRRLGFSKVFDTNFAADLTII
                     EEGNELINRIKNGGILPMITSCSPGWIKFIEHFYPKSLAHLSTCKSPQQMFGAVAKTY
                     YAEKTGIDPRNIVVVSIMPCTAKKFEARRPEMDGAYHYWKDKLKLTNEDHFYDVDAVL
                     TTRELARMFREIGIDFTHLPAEQFDNPLGESTGAAVIFGTTGGVMEAALRTAYEVFTG
                     KTLTDINFTSVRGLSGIREAEVDLDDKKIKVAVVHTLKNAGILLEQIENGTSPYTFIE
                     VMTCPGGCLGGGGQPIPTNTEIRLKRAESIYYEDAHKGIRKSHENPEILTLYKDFLKE
                     PLGEKSHHLLHTKYIWRGY"
     gene            complement(23656..26787)
                     /locus_tag="PKH58_00095"
     CDS             complement(23656..26787)
                     /locus_tag="PKH58_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX87452.1"
                     /translation="MKPHEFEILKQQFQERILPAVPMIFVGMGTCGIGNGADVIFNRL
                     EARISETKSPYLLKQTGCFGFCAQEPLVTLYQPGKPLLVYCRVNEKDAIHLVDALTKG
                     RIYNKKILCRIDEWDFRESQVHFGKGYAEIPHWDEIPFFKGQKKIVLRHAGLIDPEDI
                     RDYFAVGGYRALKKTLEMQQEDVVSEILASKLRGRGGAGFPTGIKWSIMQKAEDDQKY
                     IICNADEGDPGAYMNRNEIESDPHMLIEGMIIGAYAMGATEGIAYVRAEYPLAVKRLK
                     KAIEQATEWGLLGENILDSGFNFNLYVVEGAGAFVCGEETALIASIEGKAGRPMPRPP
                     YPAAKGLYGKPTNINNVETWCNVPVIIEKGSEWFRQTGTEKSSGTKVFSLVGKIKNTG
                     LVELPLGSTLEHFIFNIGEGTGTTKRVKAVQTGGPSGGCIPLEYFSTPVDYESLAGIG
                     AIMGSGGMVVMDQDNCMVDVARYFLEFNSAESCGKCTPCREGLSQALAILTRITKGQG
                     TFADLERLELLGRTIKDTSICALGQTAPNPILTTMKYFRKEYEEHIREKRCHAGVCDT
                     LYMALCENSCPMHMNIPAYIELIKEDRLDEAFEVTLRDNPLPGTIGRICHFHCQMRCR
                     REQLDEPVSQGEIHRYLADTIYKLGKENEIYNRLIKEKLKPTGRKIAIVGAGPAGLTA
                     AYYLVRLGHEVTVYEALHEAGGVLRFGIPQYRLPKSVLQKEVQFIRKLGVKFLFNQRL
                     GQNFSFETLIDKYDAVYLAIGAWKDIDPYIPGEKAKGVFAGTEILKELALGKMPKLGE
                     RVVIIGGGNVAIDAARSLWRLGKDVAVVYRREKGDMPANVAEIFEAEAEKINYHFLAA
                     PHEILTDARGKVKALKVEVMSGGSIDTSGRRRPVSTGKFEEIPCDSVVLAIGERVDSA
                     HLSQHGLAATKDGRLIVDPFTFLTSVTKIYAGGDSVTGPSTAAEAMGMARKAAESIDL
                     ALMNENRFHMLFRKFEYKNLVPQNPKVSPKNRSAKLTVKERIGNFQEVECGYTGEQAR
                     NEVNRCLRCDVRC"
     gene            complement(26855..27391)
                     /locus_tag="PKH58_00100"
     CDS             complement(26855..27391)
                     /locus_tag="PKH58_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNX87453.1"
                     /translation="METLTNSPVPENILDQIIGKHKGKPGILLSTLEEVQEKNPLKYL
                     PVETLTAVSQKLKVPYTQVYSVVTFYSFFNLKPQGKHSIVVCRGTACHTKGSKALLDE
                     IGPILNFKKDENDTESSYTTPDNLFTIKTVACFGQCAQSPVVEVDGVIHSNVNSQKLI
                     KMLNRLKLQETRVKPNKN"
     gene            complement(27697..29064)
                     /gene="miaB"
                     /locus_tag="PKH58_00105"
     CDS             complement(27697..29064)
                     /gene="miaB"
                     /locus_tag="PKH58_00105"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNX87454.1"
                     /translation="MKENKAVGSPDSKSALNDKKLFIETYGCQMNVADSEVVASIMQM
                     DGFELTGQITEADAVFVNTCSIRDNAEQKIIQRLKYFQSLRKKNKKLVVGVIGCMAER
                     VKEDLIENHGVNIVVGPDAYLDIPNLMGHVMNGEKAINVELSKTETYRDVIPSRISNP
                     ISGFISIMRGCNNFCSYCIVPYTRGRERSRDVESILNELKDLQEKNFKEVTLLGQNVN
                     SYQFEKDGQVIDFPQLLKIVAEAAPEIRFRFTTSHPKDMSDHTLEVIAAHPNLCRFIH
                     LPVQSGSNKILKAMNRKYTREWYLDRIAAIRRILPDASIGTDVFCGFHGESEEDHAET
                     LSLMREVGFDMAFMFKYSERPGTHAAKHLPDDVPEEIKIRRLNEIIALQNELSHASNL
                     RDIGHEFEVLIEGFSKRSKEQFFGRTSQNKVVIIPRNGRKIGQTVRVKITDASSATLF
                     GEVLN"
     gene            complement(29188..29337)
                     /locus_tag="PKH58_00110"
     CDS             complement(29188..29337)
                     /locus_tag="PKH58_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87455.1"
                     /translation="MNRLYVLLKSRCGCSRELFDKIKSVRKAAPRTNIVTLHLGNFKN
                     DDAVI"
     gene            29579..30061
                     /locus_tag="PKH58_00115"
     CDS             29579..30061
                     /locus_tag="PKH58_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87456.1"
                     /translation="MEDLGDYIYLILLVLAGISGIFKKKGKSNETGEKKKPVINLPKS
                     WEEFERQTTMQTPKTITRPEPSRTIISPARTAVPEAVSGETIPRGFETGSFQTYDAVD
                     DISKMRAKKTVTKNLKSQTYNPEPEISEEEINPLYILNNAESARAAFIYSEIFNRKYH
                     "
     gene            30212..30682
                     /gene="greA"
                     /locus_tag="PKH58_00120"
     CDS             30212..30682
                     /gene="greA"
                     /locus_tag="PKH58_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNX87457.1"
                     /translation="MAVTYMTAEGYKKLVDELNEMENVQRPAISKQIAEARDKGDLSE
                     NAEYDAAKEAQGMLEMKIAQLKDIIGSARMIDETKISTNEVQILTRVKIKNTKSGQVM
                     SYLIVSESEANFKEGKLSVTTPIAKGLLGKKVGEKATVMVPSGSMEFEVMEIGL"
     gene            30788..31189
                     /locus_tag="PKH58_00125"
     CDS             30788..31189
                     /locus_tag="PKH58_00125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNX87458.1"
                     /translation="MATIFSRIIAGEIPSYKVAEDENFYAFLDINPMSKGHTLVVPKK
                     TEDDYLFNLDNETYTGLTLFAKKVAKAIEKSVDCKRIGVAVMGLEVPHTHIHLIPINK
                     ESDMNIGNPKLKLSPGEMNEIAQKIRNNFLN"
     gene            31245..31421
                     /locus_tag="PKH58_00130"
     CDS             31245..31421
                     /locus_tag="PKH58_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX87459.1"
                     /translation="METVEKVLNAIKQASEPVNAGKVVELTGLDRKEVDKAMTKLKAT
                     GDIVSPKKCFWTAK"
     gene            31520..32038
                     /gene="rimM"
                     /locus_tag="PKH58_00135"
     CDS             31520..32038
                     /gene="rimM"
                     /locus_tag="PKH58_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445025.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNX87460.1"
                     /translation="MIQKNDIFPIGRLTKTHGLKGEMSFSCTTTILEDIEIPYVVLEP
                     EGIPVPFYIEEIRLKTDESGIIKFERIDSEEQAREYTGLTIFLPNEYLNEINEAGADA
                     DYFIGFEVSDNEKGKIGTIAGIDQSTENVLFVIETEDDEILIPAVDEYITDIDHEKKI
                     IRLSLPDGLLDL"
     gene            complement(32064..32891)
                     /locus_tag="PKH58_00140"
     CDS             complement(32064..32891)
                     /locus_tag="PKH58_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX87461.1"
                     /translation="MESIKPMRLSVEDIIREKAPNKKFPAFVVNYLKKIVHQDELNRF
                     FMSVPDKKNLEFIEAAIYEHLNAGADFEGVENLPPKDGRYIFVSNHPLGGLDGVTLGL
                     MLGRKYDGKVKFFANDILMFLEPLKEMFLPVNKVGAGSANSRVNARVVDEFFSSDFHL
                     ITFPAGTCSRKIGGKIQDLPWKKSFVTKAVQYQRDVVPIYFEGRNSGFFYNLSKIRTR
                     LGLPNIEMLYLVNEMYKQRGNHFKVKIGKSIPYQTFDSTKTPAEWAAVVREKVYEMR"
     gene            33281..33739
                     /locus_tag="PKH58_00145"
     CDS             33281..33739
                     /locus_tag="PKH58_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNX87462.1"
                     /translation="MSTFIGKYDAKADVKGRIFIPSCYRKLLSEGEREKLVMRKDPDN
                     DCLILYPESIWNKKVDDFKSRLDDWNAEDQMLLMQFVSDAEWLDIDSQGRVLITKKYL
                     QLIGIENNEVLFVGSMDRFTVWGKSRFEQAKRSPAEFASRLKDKMTKKAE"
     gene            33740..34648
                     /gene="rsmH"
                     /locus_tag="PKH58_00150"
     CDS             33740..34648
                     /gene="rsmH"
                     /locus_tag="PKH58_00150"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNX87463.1"
                     /translation="MPENPYHIPALLTETIDGLNINPDGIYVDVTFGGGGHSGEILSR
                     LSKKGKLIGFDQDEDVMKNIIDDQRFIFVKSNFRYLTNFLKYHQIEHVDGILADLGVS
                     FHHFDESDRGFSFRYDGKLDMRMNKLSPITAATILNTYEEDKLANVFYLYGELRNSRK
                     IAATVVKARAQAPITQIQEFVEILKPFFFREKEKKDMARVFQALRIEVNKEMKVLQEL
                     LNQSVELLNPGGRLVVLTYHSLEDRLVKNFIRSGNFEGELQKDFYGNVLSPLKAVNNK
                     VIVASDEEIAQNPRARSAKLRIAEKQ"
     gene            34650..35129
                     /locus_tag="PKH58_00155"
     CDS             34650..35129
                     /locus_tag="PKH58_00155"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNX87464.1"
                     /translation="MNWLKKISDTIKGSEDFSDIKSSTLRDILNGNILKKKFIQKHYA
                     LFILVAFLAFIYIDNRFYCETQVSREVKLKQQLKDIKYESLTISAQLTTLSRRTYVMN
                     FINEKGLNLKESPYAPVIITEPDPEKDQELLLQKKEEQEATKKIENDTTIVEEYIIN"
     gene            35209..37281
                     /locus_tag="PKH58_00160"
     CDS             35209..37281
                     /locus_tag="PKH58_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNX87465.1"
                     /translation="MLLVIYNIVVIQTVERKEWLALGTKNKKTDIIVQPNRGNIYSSD
                     GRLMASSIPTYYVFMDLRVPALKEKDGKLFKENIDSLSLCLADYFKDKTQAEYKRDIK
                     SAYRKGKGEYAFYKGKITYAQLKDIKKFPLFRLGKNKSGLLTKEMFRRVKPFTTLASR
                     TIGDIYADETKGGKNGLELSFDSILRGSPGVSSRQKVANRWEEVVQVSPVDGMDITTT
                     IDVDMQDIAEKALIDKLKEIEAQTGYAILMEVSTGEIKAIVNMQRNADGSYTENKNGA
                     VSDKVEPGSTFKTVSLMAVLDDGKAKLSDVIATGNGIYAFGRAIMKDHNAKKGGYHDI
                     TLEQSLNASSNIGISRTIVNAYGNNPAAFVNKLYKMKLNEPFNLYIPGTAAPNIRHPN
                     DKSSYWSLSTLPWMSIGYEVQIPPIYTVAFYNAIANNGKYIEPLFVKSISKNGQIIKQ
                     FTSRIINERICKPSTLKDVRQALLGVVENPKYGTGKAVHSPYVRIAGKTGTAQISKGS
                     QGYKSGGTTHQVSFCGFFPFDKPKYTCLVVIREPKIGYPSGGLMAGVVVKNIAERISA
                     IDTVATVQTMPVDSNYLDIKKPVLKSGKLDAINTVMTNLNQKYTGKKSDWVKTTSDGK
                     TVHVNELSINNNTVPDLTGMGAKDAVYLCERLGLKVNFTGLGKVISQSLTPGKTAYRG
                     QSITLTFK"
     gene            37307..38755
                     /locus_tag="PKH58_00165"
     CDS             37307..38755
                     /locus_tag="PKH58_00165"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNX87466.1"
                     /translation="MNLADLLQDIFIEQITGNSDIEITGIQFDSRLVEKGNMFVATRG
                     TASDGHNFIQMAVEKGAVAVVCEELPAELSDRITCIQVKNSADALGHLATCWYGFPSS
                     KMTLVGVTGTNGKTTIATLLYKLFRKLGYGSGLLSTVCNYINDDAVEATHTTPDALEL
                     NALLAKMVDAGCKYAFMEVSSHSVEQRRIAGLEFDGAIFTNLTRDHIDYHLTFENYLK
                     AKKRFFDDLDTNAFALTNIDDKNGSVMLQNTRAKKFGYSIRTMADFKTKILEDGFDGM
                     LLDMNNHEVHVSFIGKFNAYNLSAVFGAAVLLGQDEMEVLRIISSLQPVSGRFETLYS
                     PTGYAAIVDYAHTPDALNNVISTINEILRGKGRLITVVGCGGNRDKGKRPMMAREAVD
                     GSWKAILTSDNPRNEDPQDILNDMLDGLDASQKSRSLTIVDRREAIKTACSIAQKGDV
                     VLVAGKGHEDYQIIQGVKHHFDDKEEVRKCFQ"
     gene            38842..>39923
                     /locus_tag="PKH58_00170"
     CDS             38842..>39923
                     /locus_tag="PKH58_00170"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNX87467.1"
                     /translation="MLYHLIHYLDKYYDIPGSGMFLSISFRTGLAFIMALLLSTMIGR
                     KIIDKLQKLQIGETIRDLGMEDQLQKKGTPTMGGIIIIISILIPTLLLADLTNIYTLL
                     MIITCVWMGAIGFLDDFLKIKYKNKEGLKGNYKIVGQVGLGLIVGLTLYLSPDAVIRE
                     NAEIKGKSARVEDVRYASQDVKSTKTTIPFMKNNNLDYADAFKWGGDNAQKYGWIFFV
                     LMAIFIVTAVSNSANLTDGMDGLATGSSAIIGVTLGILAYVSGNVNYAGYLNIMYIPG
                     TGELLIFAGSLIGATVGFLWYNSYPAQVFMGDTGSLTLGGIIATFSIAIHKELLIPIL
                     CGLFFVESISVIVQRFYFKYTKKRFG"
     assembly_gap    39924..40023
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <40024..41331
                     /gene="murD"
                     /locus_tag="PKH58_00175"
     CDS             <40024..41331
                     /gene="murD"
                     /locus_tag="PKH58_00175"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNX87468.1"
                     /translation="GESGAGAAVLGKKQGYDVFVSDMGEIKPEYKKLLDDHEIKWEEK
                     KHTEELILQADEVIKSPGIPDKAPIIQKLKAQHTPIISEIEFAGRYTDAKMICITGSN
                     GKTTTTMLIYHILKNSGLNVGLAGNVGNSLALQVAENSYDYYVLELSSFQLDGMTEFK
                     ADVAILLNITPDHLDRYNYEFQNYIDSKFRIIQNQTEKDAFIFWNDDPVIREELKKRD
                     IHATLFPFSLQREEKTKAFIENEQLIIKTLNTIFSMPANELSLKGMHNTFNSMAAGLA
                     ASIVNIRKENIRQSLQDFQGVEHRLEYVATVKNIKFINDSKATNVNSCWYALQSMKTP
                     VILILGGTDKGNDYTEIEELVREKVSGLIFLGVDNSKLHNFFDGKIERIKDVNSMEEC
                     VKEAYNMAKPGDTVLLSPCCASFDLFKNYEDRGKQFKICVRNL"
     gene            41394..42641
                     /locus_tag="PKH58_00180"
     CDS             41394..42641
                     /locus_tag="PKH58_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNX87469.1"
                     /translation="MENLFKKYLKGDTIVWTVFFILCIFSIVELYSASSTLAFKAANH
                     TAPIIQHFSYLFVGAVVAFLVHLVPYRYIRILAFFGLFISILLLIYVLLKGPDTNDAS
                     RWIRLFGIQFQPSEIAKLSLIVVVADFISRIRANPADEKRNFWIIMLVAGIICGLILL
                     ENFSTAAILGLTIYFMMFIGRISWQKLASILGVGVILLILGYGLVKTVPEKKMPKMFH
                     RAYTWVARVDRSAEENDKDKYVINDENLQVQHGRIAVARGGFFGVFPGNSVQRDFLPH
                     AFDDFIFSIIIEEMGLLGGIFVMFLYLVLLFRTGQIATVCKSTFPAMLVTGLGLLITL
                     QATVNMFVGSGFGLVTGQPLPLISRGGTSILINCVYFGIILGVIREIKAENEKKTKQK
                     DDIDKSQQESDTEEDYVLDIEEI"
     assembly_gap    42645..42744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42745..43668
                     /locus_tag="PKH58_00185"
     CDS             <42745..43668
                     /locus_tag="PKH58_00185"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNX87470.1"
                     /translation="VRGFDRKNLLKNVSVIIDLLKSTRKAKKIIKNFAPDIAIGVGGY
                     ASAPTLKAASGYGVPTLLQEQNSYAGVTNKLLAAKASKICVAYEGMEKFFPADKIILT
                     GNPVRQDLFDVATKAKEAYEYFGFSPTRKTILIVGGSLGARTINLSIKNGLDKLTEKD
                     VQIIWQTGKLYAADARAAASTYQTEKLVVTDFVSRMDYAYSIADLVISRAGASSISEL
                     CLLAKPVILVPSPNVAEDHQTKNALALVNKDAAIMIRDMDASNELVPKTLELISDNPT
                     LERLSKNILQLALPNSADRIAEEVFKIIKKK"
     gene            43681..45054
                     /gene="murC"
                     /locus_tag="PKH58_00190"
     CDS             43681..45054
                     /gene="murC"
                     /locus_tag="PKH58_00190"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNX87471.1"
                     /translation="MTYTRYYFIGIGGIGMSAIARYFHVKGFKVAGYDRTETKLTSDL
                     QAEGIQITFEEAVSTIPSDFLEKEKTLIVITPAIPQDHEQLVYFKEHNFTIMKRSEML
                     GEITRQSKAICIAGTHGKTTTSTITAHLLYQSHVNCSAFLGGISNNYNTNLLLSNDSN
                     FVVVEADEYDRSFHRLTPYMAVIISVDADHLDIYGSEEAFLESFEHFTSLIAPGGTLV
                     INKNVHITPKLKKGVRMFTYGLNSSADFYAKNIRMEKGNILFDFVTPSDTIYDVRLGV
                     PVMINVENSVAAMAMAWLNGVTNEEIRTGISTFSGIYRRFNIVYKTDKIVYMDDYAHH
                     PSELKESISSIRQLYPGRKITGIFQPHLYSRTKDFATEFAQSLSNLDDVILLPVYPAR
                     ELPIEGVSSDLILKDVTAQHKTLSTKENLLTLLDEKNLDVLVTFGAGDIDKLVPEIKK
                     YLKKRYK"
     gene            45112..45849
                     /locus_tag="PKH58_00195"
     CDS             45112..45849
                     /locus_tag="PKH58_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HNX87472.1"
                     /translation="MNKILKYILLSVAVLLCLGYAVFAAANGNTTAKEQVCSALKVHI
                     NDFDHRQLISNTEIAKLLESEGLNPMGKTLKRIKTKSIEDAIEKHPMVRQAECYKTPG
                     GEIVVNIEQRIPVLRVVGFENYYVDDLRKPMPVSQNFAAYVPVASGRVTRKMATGSLF
                     DFCEYINKDPFWNNQIEQIHINDNLDVELVPRVGDHIVMMGDFSRYQQKLEKLKKLYL
                     YGLNQIGWNQYKTIDLQFKDQVVCTKK"
     gene            45877..47154
                     /gene="ftsA"
                     /locus_tag="PKH58_00200"
     CDS             45877..47154
                     /gene="ftsA"
                     /locus_tag="PKH58_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNX87473.1"
                     /translation="MSKNTIVHIDLGGSHITALAGIVLDSGELKILGEQTRASDDVKS
                     GIVEKISGAAYKINETTKLLQNALRIDPITSVSITVNAKSMKQIPYTLKANIRKPVTE
                     GMLKELEKECKQEIKNDKTNVIECIPLAYYMDGKRIADPIGQKGYNLRTDYNLIVCNS
                     LVTETIERSIERTGIAVDYIHLGMEAIATAVLEEDDREKGCAIISFGSTTTTLAVYHE
                     GVLQELMVVPFGGHNITKDIEELGISFSNAELLKCKTGSAMEELITKPVNVQIPNVDP
                     TREPILISTKFLSTIINARLDEILEPILGQIDQIAYPLKAGIVITGGASKLKNLNEYL
                     TKRTGLQVRTGSHAEWLSYDTDPKYKDPVYSQAIGAILLTDDKKKQKGEEEKVIQSRI
                     PSKNIFTKAGLKFKEKMNDLFDYDDIEQEKNNQ"
     gene            47248..48573
                     /gene="ftsZ"
                     /locus_tag="PKH58_00205"
     CDS             47248..48573
                     /gene="ftsZ"
                     /locus_tag="PKH58_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNX87474.1"
                     /translation="MDFLEDTLPLDLPLANSSIIKVIGVGGGGGNAVNHMYKQGIADV
                     SFVVCNTDNQDLVRSPVPIKVQLGKSITQGLGAGGRPEIARQAAEESVEDIHKMLADN
                     TKMVFITAGMGGGTGTGASPIVAKTAHDMGILTVGIVTIPFAFEGNLKIRQALEGVVA
                     LSEYVDAILVINNEKLKNIYPDLNLSNAFAKADDVLTNAAKAIAEIITISGYINIDFA
                     DVSTIMRDGKVAIMNTGYASGENRITRAIEDALTSPLLNTNDVKGAKKVLLSLYCSHS
                     NEIKMEEITQIHEFMAKVGENVTVIWGASFDDELGDEVKMTLIATGFDVKDIPGMPSG
                     LIKKRKTVEEKKENAIEVIEEPVSEEELEFNKKSSEEEFNKAMGELYGPSLRQIKTEK
                     KEEVIQEILQFEAAPEDKLPVVNIFELESEEDLNQVEQVPAWKRRFMKK"
     gene            48623..49081
                     /locus_tag="PKH58_00210"
     CDS             48623..49081
                     /locus_tag="PKH58_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNX87475.1"
                     /translation="MEEKIFEQISADIKTAMLARDKVSLEALRGIKKEFIEAKTAKGS
                     DGVLHDDQAVKIMQKMVKQRKETAQIYKEQSRPELAENELSEAAVIEKYLPQQMSDSE
                     LEAAVSNIIAQIGAAGPQDMGKVMGIASKQLAGKTEGRLISEKVKALLNK"
     gene            complement(49163..50707)
                     /locus_tag="PKH58_00215"
     CDS             complement(49163..50707)
                     /locus_tag="PKH58_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HNX87476.1"
                     /translation="MSKRFFYATLAFLFLNIAVSGQNSGDITIKILPEQGNQIISKNI
                     YGQFSEHLGSCIYGGIWVGEGSSIPNTNGYRNDVLNALKNLKIPVLRWPGGCFADEYH
                     WTDGIGPREKRPKMVNNNWGGVVEDNSFGTHEFLNLCEMLGCEPYISGNVGSGTVEEM
                     AKWVEYMTSDGDSPMANLRRANGRNKPWNVKYLGVGNESWGCGGNMRPEYYADLYRRY
                     STYCRNFDGNNLYKIASGASDYDYNWTDVLMKQVGDRMNGLSLHYYTIRDWNDKGSAT
                     QFNGDDYYKTLAKCSEIEDVLIKHIGTMDKYDPKKKIGLLVDEWGTWFDVEPGTNPGF
                     LFQQNTLRDAFVAAISLNIFNKYSDRVRMANIAQIVNVLQSMILTKDDKMVLTPTYYI
                     FDMYKVHQDATYLPLEINSDTKTVNNKELKMVTASASQKDGLIHITLTNADLDNAKKV
                     KIDLAGKKAAKVTGKILTSKTVQDHNTFENPDKVKPEAFTKFKFTDGVLNVNLPEKSI
                     IVLEIK"
     gene            complement(50883..52007)
                     /locus_tag="PKH58_00220"
     CDS             complement(50883..52007)
                     /locus_tag="PKH58_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNX87477.1"
                     /translation="MKHIRLFSVAIVITLFTGCTVQKVSQEENRIINIIHQVNDYWQS
                     SHPAQQKAFWDNAAYHTGNMEVYFLTKDKKYLQYSLDWAEFNEWKGAKSDDKTKWKYT
                     YGETDDYVLFGDQQICFQVFADLYKISPEPKKIARAREVMEYQMSTSQNNYWWWADGL
                     YMVMPVMTKLRNITGNNLYSEKLNEYLKFSDSIMYNPESGLYFRDAKYVYPKHKTLSG
                     EKDFWARGNGWVFAGYAKVLNDLPKNDKYRKQYIQRFQTMARAIANCQQPEGYWTRSM
                     LDKNQSPGPETSGTAFLTYGLLWGMNNGLLDKKTYRPVIEKSWNYLTKVALQPDGRVG
                     YVQPIGEKAIPGQIVDARSTADFGVGAFLLAACEMVRFIK"
     gene            complement(52016..53302)
                     /locus_tag="PKH58_00225"
     CDS             complement(52016..53302)
                     /locus_tag="PKH58_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HNX87478.1"
                     /translation="MSGRKLLFLFLIITVTAGKAQKLMDTGGMAYSRNSVNTTVFRSN
                     SVVSSGKYQYIAFYDQDGFIVLGQRKITEKKWKFEKTRFTGNCADAHNVISIMADDDG
                     YLHMSFDQHDRPLNYCRSLQPNSLKMGDKEQMTGNDENKVTYPEFYRLRNKNLLFVYR
                     SGASGRGNMVMKTYNLKTKTWENMHSILIDGQNRRNAYWQLFVDHAGTIHLSWVWRES
                     PGVETNHDLCYARSLDNGKTWEKSNGEKYTLPITAESAEYAWKIPQKSELINQTSMTA
                     DSNGNPYIATYWRDSTTQVPQFRVVWFDGNKWKQQQVTNRSTPFSLSGGGTKMIPVSR
                     PRIVIDKKNQIKIIYRDAERDSKVTLASCSDINQGIWNFKDMTGFQVGAWEPTYDTEL
                     WRTKNRLQVFVINTAQGDGEKLTDLTAQPVYILNIK"
     gene            complement(53328..54593)
                     /locus_tag="PKH58_00230"
     CDS             complement(53328..54593)
                     /locus_tag="PKH58_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HNX87479.1"
                     /translation="MIRRNFIKILGVTTAGTLLPQISQSSTFSAIKNKQKMQKSIPDR
                     EYFWKLLYKISEPVLRNMSNGELQKNMTVELSPSWDGRDMKVTYMECFGRLMAGLAPW
                     LTLPDDNTEEGKCRRQMKEWALKSYAHSVDPQSPDYLLWRKEGQPLVDSAYFSNALMR
                     APQQLWEPLDSVTKARIVTELKQLRRVKPPITNWLLFAAMNEAFLLSIGEEYDSEKID
                     FALKKINEWYVGDGWHSDGDKFHMDFYNSYVMHPMMLEVMEVLVKNGKAEKSAYEQTL
                     KRIQRYSEHLERMISPEGTYPPLGRSLTYRTAVFQPLALLAWRKQLPAKLTEGQVRSA
                     LKAVHKSIFNNSSNFTSDGYLTIGFAGHQPTLGDWYSNNGSMYITSESFLTLGLPASD
                     TFWTAPEEAWTAKKAFRNETFNKDYYVNY"
     gene            complement(54590..>55456)
                     /locus_tag="PKH58_00235"
     CDS             complement(54590..>55456)
                     /locus_tag="PKH58_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNX87480.1"
                     /translation="GRNLSKKLLERGYEVSVLSRSSKPARGCKVFLWDPEHQQLEDEA
                     IDTADYIIHLAGENIGSKRWTAKRKKEIIDSRVLTAKLLYDKVIERKRSIQAFISASA
                     TGYYGMVTADKVFIETDLPSDDFTGTTCRQWEEVASGFQKADIRTVIIRTAVVLDKQN
                     EALSKMIFQIKAGVGAAVGSGRQYLPWIHISDLCSIYIKALEDNNMSGVYNAVAPEQI
                     TNKEFMKKLSMVLHKPFWLPNIPAFFLKIIFGEMSGLLLEGSRISSEKIQAAGFEFVY
                     PGLENALKNILK"
BASE COUNT        16460 a        11752 c        11387 g        15557 t
ORIGIN      
        1 gttcagcaac aatttcttca gccagaaata tggacgctga ggaagaaaat tttccgggat
       61 tttccgtagt caggtaacga caagaggcat tacgcgaaca tttttcttcc atttcgggat
      121 gacgttgcag gtaatttgcc agacttccgg ccacaatttc tgcctgtgaa ataatcttaa
      181 ctccttcagg aagttgccgg gcaattttat cgtacaacag cggataatgt gtacagccga
      241 gtataacact gtcaatctga ctgtcctgat tcagcagatt gtggatatat ttttttacaa
      301 aataatcggc accttcattc tgatgttcgt tattctcaat caggggtacc cacaacgggc
      361 aggcttcaga actgacaacg atatccggat cgattttatg aatttccagc gggtaagaat
      421 cggacaaaac agtcccactt gtagcaagca gcccgatatg cttagttttg gtgaatttcg
      481 caacttcctc cacagttgga cgaataactc caagcactct tcgattaggg tctattttac
      541 tcagatcgtt ctgctggatg gttctgagtg cttttgccga agcagtattg caagccagta
      601 ttaccaaatg acagccttgc ctgaacagat attcgacgca ttgcagcgta aaccggtaaa
      661 caacatcgaa agaacgggtt ccatagggag tacgggcatt gtcacccagg taaagataat
      721 catatccggg taattctttt ctaatctgct caagaatggt gagtcctccg tatcctgaat
      781 cgaaaatacc aattggtccg gaaatttttg tcatggctgc tggttattgg ttattgatga
      841 gttgttgaat tgttgaatcg tttaatcgtt gaactgttga attgttgaca ataaattata
      901 aactatcaat atcttgtgtc ttgcatcttg agtcttgagt cttgtgtcct ggttctttgc
      961 tcctggttct tgcgtctatt tgtctagtca aacatctgac ctgcattgtt gctcaactga
     1021 accattttgt catattcttc gggtgtaaga tccaaaaagt aatagttctg cggattcata
     1081 atttcgcctt tgtaatgaac ttcgtaatgc agatgcggtc cggtggactt tccggtgttt
     1141 cccacaagtc cgataacctc gcctctgttt acggcttgtc ccgcacgcac tgaaatatta
     1201 ctcatgtgcc cgtacaaggt gacgtatcca aatccgtgat caatttcaac acagttgccg
     1261 taaccctgct gccagccagc acttttcacc cgtccgtttc cggttgcata aatatcggta
     1321 ccggtgggcg ccgtgaaatc catgcccgcg tgaaaccgcc gtgaatggta gattgggtca
     1381 atgcgcattc cataacccga tgccacacgg gtaagatcct tattagcaac aggctggata
     1441 gccggaatag cttcaagcat tttttctttg ttttgtgcca gcacaactat ctcgtcgtac
     1501 gatttggact gaacatacaa ttgttttctg agttcgttga gttttttggt ggtttcaact
     1561 acaatctgcg aattagtcat ccgaagcaac tggtcgtagt aagcagcatc catactggct
     1621 gttcgccgga cttcataggg tattggttct gcctgaaata caacacgata caaattatca
     1681 tcgcgttgct gcaaatcggt gagtacaccc tgtatatcag acatctggcc ctgtaatgct
     1741 ttatactgac cttccatacg gcttttggca agcgcagcct ctttctctcc gggggacttg
     1801 ataatagata caaaaagcag gaaaaacaaa agtccaacga aaataccgga catggtatga
     1861 aacagaatgc gttttaaccg atatgtcaga tttatcttaa tttcctcata gtctagtgtt
     1921 tcgggattga agtaaaattt ctttttggcc ataaaacaat gtgatgaaat acagattatg
     1981 ccggaagacg cgggttgatt gattcaacta ttcgtgccaa atcaatatgt ttttgcacaa
     2041 ataatggtgc aaaagtaata attttgaagt acttttgtag cttaatttga gaaatattaa
     2101 cgactgggct attcaaatga gaaacagact aatagccaca ggataaattt aagaaaatca
     2161 ttagtttcaa ttccatacta tgacatcaaa agagattaga cagtcattcc tcgatttttt
     2221 cgaaagtcac ggacataaaa ttgtgccttc agctcccatg gtaatcaaag atgacccaac
     2281 gcttatgttt accaatgcgg gaatgaatca atttaaaaat attattctcg gcaatgaccc
     2341 tataaagtat tcacgtgtgg ccgattctca gaaatgtctg cgcgtgagcg gtaaacacaa
     2401 cgacctcgaa gaggtgggtc acgacacata ccaccacacc atgttcgaaa tgctcggcaa
     2461 ctggtcattc ggagattatt tcaagaaaga agccatcaca tgggcatggg aatacctgac
     2521 tgaagtactg aaactggaca aagaccgctt gtatgtcacg gtattcgaag gttctgaata
     2581 cgaaaagttg gaacgtgata acgaagctgc agaaatctgg tcgcagtttc tgcctgctga
     2641 acgcatcata aacggcaaca aaaaagacaa cttctgggaa atgggcgaca caggtccatg
     2701 tggaccctgt tcggaaatac acatcgacat tcgccccgac agtgagcgcg ccaaagtcga
     2761 cggacttagt ctggtaaatc aaagccatcc tcaggtaatt gagatctgga acaacgtatt
     2821 catgcagttc aaccgcaagg ccgatggttc ccttgaagaa cttcccgcca aggtaattga
     2881 caccggtatg ggtttcgagc gcctttgtat ggccatacag ggtaaaacat caaactatga
     2941 caccgatgtt tttcagccta tcattgaaga aattggcaaa ctcagccaga taaaatacgg
     3001 ggaatcggaa aaaactgaca tcgccatgcg cgttgtagcc gaccatgtgc gtaccatcgc
     3061 cttctccatt gccgatggtc agctgccctc caatgctaaa gcaggatatg taatccgaag
     3121 aattttacgt cgtgccgtac gctatggcta tacattcctt gaacaacgtc aggcatttat
     3181 gtaccggtta atgccggttt tatgcgaagt gatgggaggc gcgtaccccg aactgacagt
     3241 tcaaaaaagt ctgattgaaa aagtgatgaa agaagaagag gaagctttcc tgcgtactct
     3301 tgaaacaggg ataaaacttc ttgataaagt gatggacgat gctgcaaaat taaacaaaaa
     3361 agaaatttca ggtatagatg cctttacgct ttatgacacc ttcgggtttc cgctggatct
     3421 gactgaactt attcttcgcg aacacagctt tacagtcaat attgaagaat ttcatgccga
     3481 gatgcaaaag caaaaagaac gcgcacgtaa tgcggcagcc attgaaacag gcgactgggt
     3541 taccgtacgc gaaggcgaca gcgtgtttgt cggttatgac aagaccgaaa ccgaaaccaa
     3601 tatcctgcgt tatcgcaaag taagtcagaa gaataaaact ttctatcaga tcgtactgtc
     3661 cgaaaccccg ctttatgccg agatgggcgg acaggttggc gacaggggaa cgctgacggt
     3721 taataatgaa actattgaga ttttcgacac aaaaaaggaa aacaacctat cggttcatct
     3781 caccaattcg cttcccgaag atgttaccgc accctgtttt gtccggattg atgtagcaaa
     3841 acgcaaggct acagcctgca accacaccgc cactcacctg cttcacgaag cgcttcgcga
     3901 agtgcttggc aaacatgtgg aacagaaagg ttcctttgta agtcccgaaa gtctccgttt
     3961 cgacttttca cattttcaaa aactgacaaa cgaagaacta cgcgccgtag aaaaaattgc
     4021 caaccgtaaa attcgtgaga actatccgtt gcaggaaagc agaaacacac cgattgccga
     4081 ggcgcaggca atgggagcaa tggctctttt cggagaaaaa tacggtgaaa cggttcgtgt
     4141 catccggttt ggcagttcaa tcgaactctg cggaggtact cacgtacagg ctaccggcga
     4201 tatcggcatg gttcgtatca tctccgaaag ctccattgcc gcgggcgtac gacgtatcga
     4261 agccattacc ggcgaagcgg tggaagaatt gatgaacaac gttcaggata cgctaaattc
     4321 tgccagggaa ctattcaata atgctcccga cttatacaaa gcaattcata aaacgctgga
     4381 agaaaacagc gaacttaaga aacaggtgga taacttcatg cacgagcgca tactcaccct
     4441 gcgtgacaag ctcatcgctg acgctaaaga cgaacaggga attaaagtaa tccgattccg
     4501 taccgacctc ccggctgacg cggttaagac actttcattc cagatacgaa acctgctgaa
     4561 cgaaaaactt ttcgtagcta tcaccagttt gcatgaaggc aagccatcga ttacggttct
     4621 actttccgac gatctggttg ctcaaggata caacgcggtg aacattaccc gggaagcagc
     4681 caaatgcatt cagggtggcg gcggcggaca acctttcttt gcaaccgcgg gaggtaaaaa
     4741 tcccgaaggg ctggataagg caatggaaat gctgaagctg taaatatgcc cccaacccct
     4801 aaaggggatt tttgacggat tgctacataa tggattttac cggaagacga ataaacaact
     4861 aacttaatct ggattaaaat gattattgag taagttgtgg ttttacccat gatatatttt
     4921 gataataaat tattagcttc aacgtaatat ttaaataaat agattgataa aattatgaga
     4981 attctcgtgt taaaatcagt ttttctaatc atatttctag taggtaattt cagtataaat
     5041 gctcaggatt gggctaatct gaagcgctat cagaaggaga atgatacact tttattaaag
     5101 ggcaatgcgg aaaacagggt ggtatttatg ggaaattcca tcactgaggg atggaaaagc
     5161 atgcatcctg aattctttaa taagaatcca tatatctgtc gcggaatcag cggacaaaca
     5221 accccgcaaa tgttgctacg gtttcgtcag gatgtaattg aactgaatcc acgcgttgtt
     5281 gtcattcttg caggtacaaa cgacattgcc ggaaacaccg gtccatcgac cccggaaatg
     5341 atcatggaca atatcaggtc gatggccgaa ctggcaacgg ctaacaacat ccgtgtggtg
     5401 ttgtgctcgg tgcttcctgc atatgattac ccctggagaa aagggatgaa gccaaacata
     5461 aaaataccgg aacttaacaa aatgattaaa gcgtatgccg acgaaagacg gattacctat
     5521 ctggattatt tcggtgcaat ggccaatgaa aaaaacggca tgatcgccga atatacaacc
     5581 gatggtgtcc actgcacatc agcaggatac gatgtaatgg aaaaaatggt gatacctgcc
     5641 attgctaaag cattaaagac aaaacccgca aagaaataat aatataacaa ctgaaaaaaa
     5701 gagggtgcac aaaacgattt gagcaccctc tttttataca tataatgcat tatcatctaa
     5761 ttagttcgct aataattcct gagtatgttt aatcagttcg gaccctccta ctgctccatg
     5821 acccggaatg actatttttg cggttgagaa tttacgcatc aatttttcaa tcgtaccggg
     5881 atacgcctcc aaatctccgt ctgcaacatt tcccaggtta gtagagctaa tactcttaac
     5941 catacagcct gcaaacaata tctgctctga cggtatccag acaacaatat tgtcaagcga
     6001 atgtgcggct cccgggtaat aacaaattat ccgtttatca cccagctgca gttgcagcga
     6061 atccttaaaa ccatgttccg gcaccggcat gcctctggat ttggcaaaat caatagtcat
     6121 ttggttggcg taggacgtaa tccccctgat cctgcaggaa actcaaccca cccatacaat
     6181 cgccatgcca gtgatttgga acaaatccaa ctatctttaa tttcatggaa tcggcaagcc
     6241 atgtgaccaa ctccttcgtc agaatatcgg tagccggtgt atcgaaaaga aaagcttctt
     6301 ttccggagat aaaaaccaga ccgttggaag aaatgcggcc gtaaccgggc aactcggaat
     6361 atgaaacgtg tacaaaggca ttttcggata actggatgag ttcgatatct tctgaaacgc
     6421 ggatatgttt ataatctggc tgtgcgaaac cggtatattg catcaccatc gccataattc
     6481 agaacaacta tttttttcat aaatttattc ttaacttccc aaggtttaaa tgcgtagcct
     6541 gctggaacgg atgatacaaa tccgcgccag cagggataat aaaattcagc ccaggaactt
     6601 acaggtcttt tttttataac ttaatattca gcagatagaa cgcttttgga gcaagtttga
     6661 gttcgaagtc actgcccgga atgttgattt cttcggatgc tggaacaact gtttcgggtt
     6721 tatccagggt gttttcagca tccggtttag ttgatttcag aacggtcttc gtcacctttc
     6781 tttcagcggt tttcagacct ttcaggtgat aggttaccga tttctcactg tcagaagtat
     6841 tagctatttt gagaataatt tcatttttac cgctatcata aaccgaagaa gcatagattc
     6901 catcctggcc cgtgagtgct gttttatttt cggtggtggt cagtacattg gttcctgtat
     6961 tgtgaccgta caactgctga acgtaataat ttacagatcg gacgcttctc aaattgtcga
     7021 accagatcag atcgggtttc cattgccaac cttgaacgtg cgcaaacagg ggagcgtaag
     7081 tacacatttg cactacatcg gcattgcgtt cgaatcctgt cataaaggcg gcttcgcata
     7141 acgctgcctc gaagttgttt tttctgtttg ccgggtggca ggcatattct cccgcgaaaa
     7201 ctttgggtcc tttgcggtcg tatttgtcgt agcgtgctgc tccagataag aaccattccg
     7261 gactgcgata ataatgttcg tccacgagat cggctttcaa gcgtttcatt tcttgccatc
     7321 cgtaatcaaa gtcttttcca tcgggacttg ggcccgccga tccgatgatt ttaatttcgg
     7381 gatgtctggc tctgatggca gtgataaacg gttctaatct ttcgggatag agttcacccc
     7441 actgttcgtt gccaatggca atcagtttga gattaaaggg gaccggatgt cccatttcag
     7501 agcggatttt tccgtattta gttgtaacat ccccgttcgc aaattcgatt aagtcgagag
     7561 catcctgaac ataagggttt aattcagcta caggcacatg agcgtgaata tctttattct
     7621 gaaactggca cgccagacca caactgagaa ccggaagcgg ttctgctccg atatcttcgg
     7681 aaagctggaa gaactcataa aatcccagtc cgtacgactg ataataatcg gggaaaaagc
     7741 ggtttgggaa agtatattgc cagcggtttt cgttgagcat tctgttttca acctgtccga
     7801 tggagttttt ccactgataa cgcgaattca ggtcggttcc ttcgacaatg caacctccgg
     7861 ggaaacggaa cacgcccggt ttgagatcat aaagagcttg cgcaaggtct ttacggagtc
     7921 catttttgcg tcctttccag gtgtcaactg ggaacaggga aacgtgttcc aggtccacct
     7981 ctgattttga tgtcaggaaa atacgcagtt ttgcatcggg aagggttttc gatggcttca
     8041 ggatcaactc gttctgtttc cattccgtcg agggaatgtc taattttgca tttgctatta
     8101 cgttgctttc tgtgtcgata atttcaaccc ggatgcttcc ggttgcagct gaagcttttt
     8161 ttgcccatac agagaagcgg tattccgcat cttttttcac accgataccg aaataacctt
     8221 cgttgtcgag accggtttgt ttttcatcgt gtccggcata ggcgagccgg atgtaatgcg
     8281 gattgttttt gaaaggcccg tcggttttaa cttccacttt cccgaatgtg ctccagccca
     8341 tcagatgttc cggaaattcg aacgagcgat tttttaccag ttcggcatac aaaccaccat
     8401 cggcgccata attaatatct tcgaagaaaa tgccgtacat ggttttttga acgggaattc
     8461 catttttctt gaaatcgacc tggatattgt gagtttgagc cacagagagt tgaactccca
     8521 ggaatgtaac taataagaaa aacagtttaa tttttggtgt tttcatgatt tatgaatgta
     8581 agttgattaa atttaatatc gatttttgtg cacattaata agtgaaggtt tttcattgaa
     8641 ttcaaccccg tcggaacaaa cacaaacctt cacaagttgt tttagccttt gctcagcccg
     8701 tttggcttgt ggtgtacacc tgctgattac ataatattta aatcgtaaaa cgggtcaacg
     8761 aaattacaga tttattttac aatatcaaga tgattcaagt atgttttttg atatttcttt
     8821 ttcgataatt ggcaggttct tacattttta atcagcgtta aatatttatg ttttgtttgg
     8881 aaatcaaaaa agtccggttc tgacatcctc ggcttaaaac aaaaaaagtc cgctgcaaaa
     8941 atattgcaac ggactttctt atttacaatt tcccaactat tcctccatca aggggttata
     9001 ggacgattat taatctttga attttgctcc gaggaattct ctgttcagac gggcaatgtg
     9061 cgaaatcgaa acgtttttcg ggcattcggc ttcgcatgct ccggtgtttg tacagttacc
     9121 aaatccgagt tcatccatct ttgcaaccat tgctttggca cgttgtgcgg cttctacccg
     9181 tccctgtggt aacaatgcga gctggcttac ttttgccgat acgaatagca ttgctgaacc
     9241 gtttttacag gcagcagcac aggcaccgca accgatacac gatgcagcat ccattgcttc
     9301 gtcagcattt actttcgaaa taggaattgc attggcatcg ggagttccac cggtattaat
     9361 cgatacgtaa ccacctgcct gaataatctt atcgtaagcg cttcggtcaa ccatcaggtc
     9421 tttgataacc gggaatgctg ccgaacgcca cggttcgatg gtaatcgttt caccatcgct
     9481 gaagttgcgc atatgcagct gacaggtagt gatttctcca tcgggtccgt gaggatgtcc
     9541 gttgatatac atgctgcaca taccgcatat accttcacgg caatcgtgat caaaggcgat
     9601 tggttctttg tgctcaccaa tcagtttttc gttgaggatg tccatcattt cgaggaacga
     9661 tgcatccgtt gatatatttt ccactttata agtctcgaaa ctgcctttag ctttcggacc
     9721 ggcctgacgc catactttta gagtgagatt taaacttttt tccattatgt ttgcccccta
     9781 accccctgaa gggggaattt agaagttaat aaatgattaa tatatatata tttttgctcc
     9841 caagcctacc tgtggtagtc aactcccttt tcatggagct gtgggggtag ctgttacgct
     9901 ttataattac gttgctgacg aacaataaat tcgtaatcaa gcggttcttt gatcagttca
     9961 ggttcctgac cttcaccttt gtatttccag catgacacat acgcgaattt atcgtcatcg
    10021 cgaagagctt cgccttcggg tgtctgatat tcttcgcgga agtgaccacc gcaggactct
    10081 tcgcgcagca gcgcgtcgcg tgccatcagt tcgcctgttt cgataaaatc ggccaagcgc
    10141 agcgcttttt caagctcaac gttcagatta tcggcttttc cgggaacata tacatttgtc
    10201 cagaaatctt ttcttacctg ggcaattttc tccagacctg ttttcagtcc ttcggcagta
    10261 cgtcccatac caacataatc ccacatgata aggcccaatt cacgatgaag atgatctacc
    10321 gagtatttgc cctgaatgct catgatttta tttattctgt cattgattgc tttctcggct
    10381 tcttcaaatt ccttgctgtc ggtgctgatg cgcggaacgc gaatctgatc ggcaagataa
    10441 ttctgaatag tatatggaag aacaaaatat ccgtcggcca gtccctgcat gagtgcagaa
    10501 gctcccagtc ggttagctcc gtgatcagag aaattcgctt ccccgatgca aaacaatcct
    10561 ttaaccgatg tctggagttc ataatccacc cagataccac ccatggtgta atggatggca
    10621 ggataaatca tcatcggggt tttatatgga ttatcgtcaa cgattttttc gtacatctgg
    10681 aataagttac cgtaacgggc acgaacaaca ttctcgccca ggcggtcgat ggcttccgaa
    10741 aaatcgagat aaacggcaag tcctgtattt cccacaccaa atccggcatc gcagcgttct
    10801 ttggcagcac gtgaagcaac gtcgcggggt acgaggttac cgaaagccgg atatctgcgt
    10861 tccaggtagt aatcacgatc ttcttccttg aggtcggtag gnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn natatccaat cggcgtttgt tagccggatt aacaagtttc
    11041 tgatgggctt tgtaattgct ccatttttca gccaacggtc cttcaggaat tttagcatcc
    11101 tgtcttgtca gtattttttc cactttcgta gtttcttctt tatttgccat aatttttaga
    11161 ataaattaat cggttacaca attacatagg gcgaaatccg aaaaggaaaa gcaccggaat
    11221 agccataaat aacaaaatga tgaatgtcga ataaatgtta gcaaaacatt tcaggcgggg
    11281 caaccaggtt ttgttattca aaccgattga ctgtaatgcg ctccaaacac cgtgagtaag
    11341 atgaaaccac aatgctgcca accatacaat gtaaagaaca caatacaatg gctgactgaa
    11401 caattgaata atgaaggctg ttccgtcggc aggatgaaaa tctcctgtat gaacaccaga
    11461 caattcagca tactgcattt tataccagaa ctggaaaagg tgcagtgcca taaaaccaaa
    11521 aatgacacct cccaatatca acatattctt tgatgcccat tttacgcctt cctgacgttt
    11581 tgttacagca tatctgtttg ttccgcgtgc tttgtaattg aggattgaaa gataaagcgc
    11641 ataaatgata tgcaccacaa atcctgccgc aagcaccatt gaacctacta ctgcatacca
    11701 gttagctcct aaaaattcgc aaactgcatt gtaggcctca ccactgaaca gcaccgtaac
    11761 attcataata ctgtgaaaag tcaaaaacag tataagaaaa attcccgata cactcatgat
    11821 gacttttcgc ccaatagaag aatcgattaa ccacatagat tttctgtttt tttgtttgaa
    11881 atatagttag tatttaatta aattcgaaat caaataataa tttttccgga aagttatttt
    11941 tccataaaag tcaggctttc aggaaaagat aatgaacatt acctaaattg gggacaaaat
    12001 tactcatttg accttgatta aaaaaataat tacggaaata attccttaat ttagcatagt
    12061 aattagtatc tttgttacat aatacttttg ttatgaaatt cacacctgaa acctacaagc
    12121 ttcccgataa gcccataaca ccgttcatcg acaccgatga aatagaggga tatcttaaca
    12181 acacgattcc tatcagagaa cgtgtgcgcg aggtgattgc caaatcactg tcaaagaaac
    12241 ggttgaacct ggaggaaacg gctgtgttga tcaacgcgac tgaccccgaa cttattcagg
    12301 aaataaagga aggagccaaa caactgaaaa agaatatcta cggcaaccgc attgtacttt
    12361 ttgctccgct ctatgtgggc aataaatgtt cgaataactg tacctattgc ggtttcaggg
    12421 cttcaaatac agcagaagtg cggaaaagtc tcaccgacga tgaactgaaa cttgaaattg
    12481 aagcactgga agataacgga caaaaaaggc tgattttggt ttacggtgaa catgcacagt
    12541 acgacgctga atacattgcc cacaccgtga aagtagctta cagtgtgaaa aaaggaaacg
    12601 gtgaaatccg ccgcgtgaat atcaatgccg caccgatgga aattgaaggt ttccggacag
    12661 tgaaaaaggc aggaattggt acttttcaga tttttcagga aacatacaac cgcgaagctt
    12721 ataaaaccta ccatcttcgc ggcaaaaaag ctgatttcga ttatcgcctc acttcgctcg
    12781 accgggcaca ggaagccggt atcgatgatg tgggcattgg tgcgctgttc ggactgtacg
    12841 actggcggtt cgaagtaatg ggactggtgc gtcacaccaa ccacctggag gcttgttaca
    12901 acgttggtcc gcacaccatt tcatttcccc gggtaaagga tgcttcgatg ctcgatatga
    12961 aagatacgta tttcgtttcc gatgaagatt ttgcccgact ggttgccatt ctccgcctgg
    13021 ctgttcccta cacaggcatg atactaactg cccgcgagcc ggcagcgctg cgcaacgaac
    13081 tgatccagta cggcgtttcg caaatcgacg gcggcaccaa aattgagatt gggagttatg
    13141 tggaaaccca aaacaccaaa caggacctga acagaggtca gttcagaatt ggcgacgacc
    13201 gctcgctgaa tgaagttgtt gaggaattgc tctcacagga tatgatacct tcgttttgca
    13261 ctgcttgcta ccggttgggg cgcaccggcg aacatttcat ggagttctcc gtaccgggat
    13321 ttataaaacg atactgcacc cccaacgcca tactcacact gacggaatac ctgatggatt
    13381 atgctcctga aaaaacagcg gaaaaaggat ggaaagcgat tgaaaaaaat attctgacac
    13441 tggatgaaac aatgcaaaag caggtacgcg aacgtattga aaaaataaaa cagggagaca
    13501 gggatttgta tttttaaaaa attggagttg ccccctaacc ccctaaaggg ggaataatga
    13561 tgcaaaattt caatatattt acaaagatga aaaaaaaaca tatcactgat aaaatctatc
    13621 caacatctcc cccttcagag cctgtcccga accgatttcg ggagggccgg ggtgctttta
    13681 tcggcatttt cggtcgccgg aatacgggta aaagttcact gataaacctg ctgacgggtc
    13741 aggacattgc catcgtttcc gataaacccg gcaccaccac cgatccggta agcaaatcgg
    13801 tggagatatt tggtattggt cccgtggtat tgattgatac tgccggaatt gacgatgcag
    13861 gcgaattggg cgaaaaaaga ataaaaaagt cgaaagaagc tattaagaag gccgactgcg
    13921 cgattctggt aatcgcggaa aacacatttg ataattttga acttgattta attaaacttt
    13981 tcgacgaata cgccgttcca tacctgattg ttcacaataa aagtgatctt tcgcctttaa
    14041 ctaccgaaac catcgctgaa ataaaaagtc attcatcttc tgaaattctg gatttcagca
    14101 cgcagaacga agaaaaaaaa gaacttctca tttcattgct aaaaagtacc atcccaacta
    14161 ccaactacca aaaaccatca ttactgggag gtttggtgca gccgaaggat gtggtattgc
    14221 tgattactcc cattgacagc gaagctccgg aaggaaggat gatactcccg caaaacatgg
    14281 ctatacgaga tgtacttgaa aataattgca ttacagtggt ggtaaaagaa accgaactcg
    14341 aagatttttt caaactgggc ataaagcccg cgttagctat aactgacagt caggctttta
    14401 attttgtttc aaaaatagtc cctacggatg ttccgctgac cagtttcagc attgtttttg
    14461 cacggttgaa aggtgatttt gagaaatatc tggaaggaac accgcatatt tcacaattga
    14521 aagatggtga ccgggtgttg atacttgaaa gctgcaccca tcaggtgagt tgcgatgaca
    14581 ttggcagagt gaaaataccc ggattattac aaaaattcac cgggaaaaaa cttgaattta
    14641 ctgtggtaag cggcctttca gaagtcaccg aaaatgtgga gagtttgtca ctggttattc
    14701 agtgtggcgg ttgcatggtg acccgcaagc agctcctcaa caggctgaaa ccatttaccg
    14761 aagccgaaat tccggtaact aactacggca tggctctggc gtatgtgaac ggaatcttcg
    14821 aacgggcgac ggcaccattt ttgtagtaaa tttgttaata taaaacgctt aaatatatga
    14881 gaactctttt ccttgtttcc cttctttgca taagcagcta tgtttgctcc caaacagttg
    14941 aaattgattt atttggaaat atccagatta ataacaccat aaatgatgac tgtgacggtc
    15001 agaaaaaagt gattaaaaaa gatattttcg gaaatgtagt cattgaagaa aataacggta
    15061 aaaaagtaac agtaatcaag aaagacattt ttggaaatac aattgttgag gatggctctg
    15121 gaagaactat caacgtgttt aaaaccgata ttttcggaaa tcaggttatt gaagacggtc
    15181 agggcaatac tgttagaaaa ataaaaaaag acatatttgg aaacacaatt attgaagatg
    15241 gtcatggttt tactacatta aaaattgaaa aagatatatt tggcgatacc attattttag
    15301 atggcagcgg ccacaggatc agaaagataa aaagagatat attaggtgac cggattattg
    15361 aaaacgaaaa cggcaaaaca ataatcaggt taaaaaaaga tattctgggt aatcttattg
    15421 ctgaagatga aaccggtttc aaaattagaa cattaaagaa agatatttta aataattatc
    15481 aaatagaaga taaatacgga caaactttat ttgaaataaa gaaggacata ttaggaaaaa
    15541 tatcagtaag agatcaaaac aacaaagtag taaaagagat ttcaaaagac atattcaata
    15601 atattattta ccaggacaga gtttgcaata aacataatat cgatgcttat ctgattgatt
    15661 tcgcaataca acaaatcgga atctgacaaa ttatgtctat aacaacactt ttacataaat
    15721 ctcaattttc gaaacccgaa attgtccggc ttctttctgc tactgatgag gatgagaagt
    15781 tgttgttcga ttatgcatca gaaattaagc aggctaatgt gggcaatacc gtttttctgc
    15841 gcggattaat tgaactttcc aacatttgcg aaaaagattg ctattattgt gggattcgca
    15901 agtcaaattt taatgtaaag agatacaaaa tcagcgaaga agaaacggtg gaaaccatcc
    15961 ggtacgctta tgaaaaaggc tacggctcgg tagcaattca gtcgggtgag gtgcaaagca
    16021 agacttttac tgaaaaaatc aaccgcattt taaggaaagc gatggaaatc agtaacggtg
    16081 aattaggcat tactctctct tgcggcgaac aaaccgaaga gacgtaccgc cggtggttcg
    16141 aaagcggtgc aaaacgctac ctgctgcgca tcgaaacctc cagtcctgaa ctgtaccgca
    16201 agctgcaccc cggcgacaaa aaacatagtt ttgaaaaacg gctggaagct ctgcaaacgc
    16261 tgaaatcaat cggttatcag accggaacag gcgtgatgat cggactgcct tttcagactg
    16321 ttgaagatct ggcggacgac ctgctgttta tgaaggattt cgacatcgtg atgtgcggca
    16381 tgggaccata cattgaacat cacgaaacgc cattgtttca gtatcataat caattactgt
    16441 cctttaatga gcggctggaa ctgagtttga aaatggtggc tattttgcgt attatgatgc
    16501 ctgatatcaa tattgcagcc actaccgcac tgcaaaccat tgaaaaaaac gcccgcataa
    16561 aagccatcaa aatcggagcc aacgtgctga tgcccaatat cacgccccac cgttaccgcg
    16621 acgattactt cctctacgaa aacaagccag tctccggtca aagtagggaa gatgatctgg
    16681 aaaatctgga taaatcactg caagccattg tacaccggat cgggtacggt gtacagggaa
    16741 actcgaaaca ttttataagc ccctaaccca ctgagcagga aaaatctgtt gaatattcag
    16801 gattatctgg atttcatatc atcctcaaca tcaaattgtc taatcaacca cgaaaaaaac
    16861 gaggtaagct tgtgaaaacc taccccgttg ataacttaaa atgagatttt atttatattc
    16921 atccagaata tcccgaacct tatccggtga gacgcgtccg aacactttct cgcccaccat
    16981 caccaccggc gccagtccgc aggcgcccac gcagcgcagg cagttgatgg agaaacgtcc
    17041 gtcatcggta gtttcgccca ctttaatatt cagttccttt ttgaattcat cgagcacttt
    17101 ttcggctccg cggacgtaac aagccgttcc catgcagatc gaaatcgggt aatcaccttt
    17161 cggaagcatg gagaagtagg aatagaacgt aaccacaccg taaacatgcg cagcagatac
    17221 tcttagattg tgcgcaatca attcctgtac ctcagccgga aggtagccaa acgtgtcctg
    17281 cgtttgatga agaacattaa tgagttcacc cggatcattg tcaaaacggg cgcaaatttc
    17341 attgatcatg ctgatttttt cgtccgataa ccggattttt atattcactt tttccttatc
    17401 gggagccatt acaggtatag ctgtattatt ttcgcaacta ctattttctg aagccatatc
    17461 aattaattta ctatgaataa actatgaaaa attaatccag aatagacact tcctcactct
    17521 tatcgaagta atgagtatga agtaattcat gtgctttcgg gcttcctggt tctcccagaa
    17581 attcgtcgta aatccggaca atctgcggat tttcatgcga tttccgtaat gttttacgct
    17641 cgtcttcggt gtagatagcc cgcgctctct ttttcagaat atcactttgt ccgtggtggt
    17701 atggttgccc gccgccacca atacagccgc cggggcaagc cataatttca atggcgtgat
    17761 aggttgattt tccttcgcgt acttcgttta gtagtttgcg ggcatttcct aatccatgag
    17821 cgatgccgat attcagtttc agcccatcga aatcgacggt tgccgagcga attccttcca
    17881 gaccgcgcag ttcggtaaat tccagtcgtt ccagcggttt gccggtataa atttcataag
    17941 ctgtgcgaca ggcagcttca atcacgcctc cggttgtgcc aaaaataaca cctgcgccgg
    18001 tcgactcgcc cagtggattg tcaaaacctt ctgaaggaag atccatgaaa tcgatgttgg
    18061 attgctgtat gaagtgtgcc aactcgcggg tggacatgga aaaatcaacg tccggattgc
    18121 cgttgacctt aaactcatcg cgctgacatt cgtatttctt agccagacaa ggcatgacgg
    18181 aaacgacaac tatatctttt ctgtttactc ctatcttttc ggcaaaatag gtttttgcaa
    18241 ctgctccgaa catctgctgg ggagattttg cggtagaagg cacatcgagc atatcgggga
    18301 aattgtgttc aaagaaatta acccagcctg ggcagcagga agtcagaatg ggtaatttca
    18361 ccgttttatc gccattcaga tgtcttttca aacggcaaag cagctcagtc ccttcttcca
    18421 taatggtaag gtcagctgca aaatcggtat cgaaaacata tttaaaaccc aaccggcgta
    18481 atgctgccac cattttgcct gtaaccagtg ttccgggagg caatccgaaa gcttcgccca
    18541 gcgcggcacg aaccgcaggt gcagtctgaa ctattaccgt tttcgacttg tcggataaca
    18601 cgcgcagaat ttcattggtg ttatccactt cggtcagcgc acccgtcggg caaaccgata
    18661 cacactgtcc gcaataggta cagggtgaat gtaccaaatc catttcgaat gcaggcgcca
    18721 ccaccgacat aaatccgcgg ttcacagccg ataaagctcc tactgtctga acttcgttgc
    18781 acatcatttc gcagcgacgg cacatgatac atttatccac atcccgaatg atggatggtg
    18841 aggtatcttt ccggtaagtg gatttttcgc cctgataggg aatatcacga ataccaaact
    18901 tctgtgcaag gtgttgcagt tcgcattcgc ccgattttgc acacacgagg cagtcgaacg
    18961 ggtgatcgga aacgatcagt ttcagtaccg ttttgcgggc attgagcact cgagtgttgt
    19021 gggtattgat aaccattccc ggtgtacatt cggtgataca cgcgggagcc aggttgcgac
    19081 gtccttccac ttctaccacg cagatacggc aaccgcccgg tttattttca atatccatgt
    19141 cgtgaaggcg gaaatgacaa agtgttggaa tatcaattcc tgcctcagct gctgctttga
    19201 ggatggtcgt tccttctttt actcttaaaa ttttgccgtc tatcgttaat tctatgtttt
    19261 ccatgtattt gatctgaata attggttaaa aattaaagca ccgttactcg tttaggttcc
    19321 agctcgatgg tgatggcatc gaacgagcat ttttcgatac attctccgca acggatacaa
    19381 atgtccggat caatgtgatg aatttgtttc ttgtcacccg tgatggcatg aacggggcag
    19441 catcggcgac aggcaccaca gcccacgcaa agttcatcgc tgaccacgta ataatacaga
    19501 cttttgcaat gtcccgaacg gcatttttta tccaccacgt gttcgtaata ttcttctgca
    19561 aaattctcca tggtcgaaag caccggattg ggcgaagtct gtcccagacc gcacagtgac
    19621 gtatctttta ttacgttgca gaggtttttc aaccgttcca ggtcttccag ggtgcctttc
    19681 ccctgcgtaa ttttatccag catttcgtac agtcgtttgt tgccaatacg acatggagca
    19741 catttcccgc aactttcctc cacgataaaa tccaaataaa attttgccac cgacaccatg
    19801 cagtcatctt cgtccatcac aatcattcca ccggaaccca tcatggaacc tgcctgtgtt
    19861 aagctttcat agtcaatagg aatgtctaag tgtttttcgg tgaggcagcc gcccgaaggt
    19921 ccaccggttt gcacggcttt gaatttttta ccgcctctga tacctccgcc gatttcatag
    19981 ataatttcgc ggagtgtagt tcccattggc acttcaatca gtcccacgtt attaattttt
    20041 ccggccaggg caaacacttt tgttcctttt gatttttcgg ttccgatgga tgcaaaccag
    20101 tcggctccat tgagaatgat ttgcgggatg tttgcaaaag tttccacgtt attcacgttg
    20161 gtgggtttcc ccaggtagcc gctctgagcc gggaatggcg gtttgttggt tggttcaccg
    20221 cgttttccct ccatcgaatg gataagtgcc gtttcctcgc cgcacacgaa agccccggca
    20281 ccgtagcgaa gcgaaatatc gaaattaaaa ccggtcccaa ggatatcaac accaagtaat
    20341 ccgtagtttt tagcctgttc gatggcaatt ttcaaccgtt tgatagccag cggatattct
    20401 gcgcgaatgt aaatcagtcc tttgctggca ccgatgcagt aaccgcagat agccatagac
    20461 tcgagcacgg catgcgggtc accttcgagt atagagcggt ccataaatgc gcccgggtct
    20521 ccttcatcag cattgcaaac cacgtatttt tgatcggaat cgtttttact ggctatttcc
    20581 catttcaaac cacaagggaa gccgcctccg ccacgaccac gcaatcccga ttttttgata
    20641 atctctatgg tttcggcagg tgaatattcc gtcagcactt tgcccatggc cagataggaa
    20701 ttgtgcttga tgcaatgatc aatattttcg ggattaacca ccccgcagtt tcgcagggca
    20761 attcggattt gcttgcggtt cagacttttt gtaccggtaa tcactttctg atcatcggga
    20821 tgatacaaga gtctttcaac aacccggccg ttgataatgt gttcattgat aatttcatcg
    20881 gtatcttcgg gtttcacatt agcataaaaa atattccccg gaagcacttt gataatggtt
    20941 ccgctggcac acagtccaaa acaggcggtc tggataacct gaaccgagtt catcagttct
    21001 tcatcagttt caatccgcca tttgatgcgc gttatcacat cttcactgtt gtgatggcgg
    21061 cagctattgc ccgcgcaaac catcagttgc attctgaatt tttccataat ttcgatgttt
    21121 cgcgtttatc ttctttcgta attggtaggg ataattccat ccaccagttc gccggttttg
    21181 atgtattttt ctatgatttc gtctgccctt accgtatcca cataaccaaa aacaacaggc
    21241 tcgcgattgg ggagaataat ttcaacggtt ggttccgcgt agcaatatcc catacatccg
    21301 gtttgggaga tgctacaact taccgatcgt tttttgagtt cggcagtaaa atagtccagc
    21361 acttcctttg ctcccgacgc gatgccgcag gtggccatcg ctactttcac ccggacgggt
    21421 ttatcttcgc tacttttatc gggtcgccca attctcaacg tttcacgcaa ggctttgagc
    21481 tcttcatacg atttaatttt ctccataact attttgtaat aagctattta atgcgacaga
    21541 tgatgttgaa ctctgaaatt atttgttttc aaatatatgg atattgtttt gattaatgaa
    21601 ataattctgt aattaaattt ataaaatatt tattgtgtta aattacagaa acttacaaca
    21661 taccacttaa tggccaaaca ctgcttttaa gcatatttta accggtattt tgtttttttg
    21721 attacagttt gaaagcataa tcaagacagt aaaaaaaaag aagctttcat caagcctcct
    21781 tttgaaacat gtttttatag tacagtattt aatatcctct ccagatatat tttgtgtgta
    21841 acagatgatg cgatttttca cccagtggtt ctttcaaaaa atctttatag agtgtcagta
    21901 tttcgggatt ttcgtgcgac ttgcggatac ctttgtgggc atcttcgtaa taaatggatt
    21961 cagcacgctt taaccgtatt tcggtattgg tcgggatggg ctgtccgcca cctccaaggc
    22021 aaccacccgg gcaggtcatc acttcaataa aagtataggg ggaagtgccg ttttcgattt
    22081 gttcaagcag aatgccagcg tttttcaatg tatgaaccac agctaccttt attttcttgt
    22141 catccagatc cacttccgct tccctgatac cggacagtcc gcgaacagat gtgaaattaa
    22201 tatctgtcag cgtttttccg gtaaaaactt cgtaggcagt tcgcaacgca gcttccatca
    22261 ctccaccggt agttccaaag ataactgcag ctcctgttga ttcacccaga gggttatcaa
    22321 actgttctgc gggtaaatgg gtaaagtcaa taccgatttc acggaacatt cgcgccagtt
    22381 cgcgggtggt caagacagca tccacatcat aaaaatgatc ttcattggtt agtttcagct
    22441 tatctttcca gtaatggtag gcgccgtcca tctccgggcg gcgggcttca aatttcttgg
    22501 cggtgcaggg catgattgat accactacga tattccgggg atcaatgcct gttttttctg
    22561 cataataggt ttttgccacc gcgccaaaca tttgctgcgg tgatttgcag gtagataaat
    22621 gggctaatga tttcggatag aaatgttcga taaacttaat ccagccaggt gaacaggagg
    22681 taatcatggg cagtatgcct ccgtttttta tcctgttgat caattcattt ccttcttcta
    22741 taatggtaag atcagccgca aaattggtat cgaacacttt ggaaaagccc aatcggcgca
    22801 atcccgccac catttgtcct gtaacaagac ttccgtaagg cattcccata gcttcgccaa
    22861 ttccaactcg gatagcagga gctgtttgaa ccagtaccac tttttcggga tcattcaacg
    22921 ctttccacac gtcaccgatg gcgctttttt cagttatggc acccgtagga cataccagtg
    22981 cacactgtcc acaattggta caagctacga ttcccattcc caaatccata taagttgaaa
    23041 tccggctttt cagtccccgc caggcaaaat caatggcttt tactccctgc acctcgcggc
    23101 acaccgccac acaacggcca cataaaatgc atttctcagg atcgcgaacc aaagacaacg
    23161 acgattcgtc tttcggggaa tattcttttt tggtccggac aaatggtctt tcgtgaatac
    23221 ctaaatcgaa agttatttcc tgcaattcac aattctgatt acgatcgcat cccaggcatt
    23281 cctgcggatg gttagccagc ataagttcaa tgtttaccct tcgcgcttcc atcacacgct
    23341 gactgttggt ggtaatatcc ataccgtcgg ttacttgcgt gatacacgaa cgcagaagcg
    23401 atttggcgtt tttaacctcc acaacacaca caccacacga agcattgtgc gatacatctt
    23461 tgaggtaaca cagacaaggt acatgaatac caactgcatt acaggcttcc atgatggtta
    23521 aaccttcctg aacttccaga ggctgattat tgattgtgat tttcataaga ttcgaatttg
    23581 acgaattaat acgaattaca cgaatttttg aaacaaatgc aatttatgag atgatttaat
    23641 gctatcatga tagacttaac atcgcacatc acatctcagg cagcggttga cttcattccg
    23701 ggcctgttcg ccggtatagc cgcattccac ttcctgaaaa ttgcctatac gctctttcac
    23761 tgtgagtttt gcagaacgat ttttcggact tactttcgga ttttgtggta cgagattctt
    23821 atattcgaat tttcggaaca gcatatgaaa tctgttttca ttcatcagcg ccagatcaat
    23881 ggattctgct gcttttcggg ccatacccat tgcctcggct gctgttgaag gtcctgtaac
    23941 agaatcaccg cctgcataaa tcttcgtaac agatgtcagg aaagtaaacg gatcaactat
    24001 caatcgcccg tcctttgttg cagccaaacc atgttgactc aggtgtgcag aatcgaccct
    24061 ttcacctatt gccaaaacga cagaatcaca gggtatttct tcaaattttc cggtcgacac
    24121 aggtcttctt ctgcccgacg tatcgatact tccgccactc atcacttcca ctttcagggc
    24181 cttcactttt ccgcgggcgt ctgtgagtat ttcatggggt gcagccagaa aatgataatt
    24241 gattttttca gcttccgctt cgaatatttc ggctacgtta gctggcatat cgcctttttc
    24301 acgccgataa acgactgcga catccttacc aagtcgccac aagcttcgtg cagcatcaat
    24361 agctacattt ccgccaccaa taatgactac acgttcaccc agtttcggca ttttacccaa
    24421 cgccagttct ttcaggattt ccgttccggc gaaaacaccc ttcgcttttt caccggggat
    24481 atacggatca atatctttcc atgcaccgat ggccagataa acagcatcat atttatctat
    24541 taatgtttca aatgaaaaat tttgacctaa ccgctgatta aataaaaatt tcacacccaa
    24601 ctttcggata aattgcacct ccttttggag gactgatttc ggcaggcggt actgcggtat
    24661 gccaaatctc aaaacgcctc cggcttcgtg caaagcttca taaacagtaa cctcgtgtcc
    24721 caaacgcacc aggtaataag cagcagtaag accggctggt ccggctccaa caatggcaat
    24781 tttcctgccg gtaggtttca gtttttcttt tatcagtcgg ttataaattt cattttcttt
    24841 accgagttta tagatggtat cagcaagata gcgatgaatt tcaccctgcg aaacaggctc
    24901 atccagttgt tcacgtcggc aacgcatctg acagtggaag tggcagatac gtccgattgt
    24961 acccggcagc ggattgtcgc gcagggtaac ctcgaaagct tcatcaagcc ggtcttcctt
    25021 tatcagttcg atatatgccg gaatattcat gtgcatcgga caactatttt cgcacaaagc
    25081 catataaagc gtatcgcaaa cacccgcatg acaacgtttt tcacgaatat gttcttcgta
    25141 ttcttttctg aaatacttca tggttgtcag aatgggattg ggagcagtct gacccagcgc
    25201 acaaatggaa gtatctttaa tcgttctgcc cagcaattcc agccgttcga ggtcggcaaa
    25261 agtaccctga cctttagtaa tccgggtaag tatggcaagt gcctgcgaca gcccttcgcg
    25321 gcaaggagtg cattttccgc acgattccgc tgagttgaat tcgagaaaat agcgggctac
    25381 atccaccata caattatcct gatccatgac taccatccct cccgaaccca tgatagcccc
    25441 gattccggcg agcgactcgt aatccaccgg agtggaaaaa tattccagcg gaatgcaacc
    25501 tcccgaaggt ccgcctgtct gcacggcttt cacccgttta gtggttccgg ttccttcacc
    25561 aatattgaaa atgaaatgtt cgagcgtgga acccagcgga agctccacaa gcccggtatt
    25621 ttttattttc ccgaccagcg agaaaacttt tgttcccgaa cttttttcgg tcccggtctg
    25681 cctgaaccac tcacttcctt tttcgataat gacaggcaca ttgcaccagg tttctacatt
    25741 attaatattg gtgggtttac cgtacaatcc ttttgctgcc ggataaggcg gacgtggcat
    25801 cggacggcct gcttttcctt cgatagatgc aatcagcgcc gtttcctctc cgcaaacaaa
    25861 agctccggca ccttccacca cgtacaggtt gaaattaaaa ccggaatcta atatattttc
    25921 gcccagcaac ccccattcgg tagcctgttc gatggctttt ttcagtcttt ttacagccag
    25981 tggatattct gcccttacat aggcaattcc ttcggtagct cccatcgcat aagccccaat
    26041 tatcatccct tcgatgagca tgtgcggatc actttcaatc tcgtttcggt tcatatatgc
    26101 acccggatca ccttcgtcgg cattgcaaat aatgtatttt tggtcatctt ctgctttctg
    26161 cataatcgac cattttattc cggtcgggaa gccggcacca ccccgacctc gaagttttga
    26221 agcaagaatt tcactgacaa cgtcctcctg ttgcatttcc agtgtttttt tcaatgcccg
    26281 atatcctcct actgcaaaat aatcgcgaat atcttctgga tcaataagtc cggcatggcg
    26341 cagtacaatt tttttttgcc ctttgaaaaa aggaatttca tcccagtgag gaatttcagc
    26401 gtatcccttt ccaaagtgaa cctgtgactc gcgaaagtcc cattcatcaa tccggcaaag
    26461 tattttttta ttataaattc ttccttttgt cagcgcgtca acaagatgga tcgcatcctt
    26521 ttcatttacc cggcagtaaa caagcaacgg cttgcccggc tgatacaggg ttaccaacgg
    26581 ttcctgtgca caaaatccaa aacaacccgt ttgtttgagc aggtagggag attttgtttc
    26641 tgaaatacgc gcttcaaggc ggttaaaaat cacatcggca ccattaccaa tgccacaggt
    26701 acccatccca acaaatatca tgggaacagc aggaagtatc cgttcctgaa attgctgttt
    26761 taggatctca aattcgtgtg gtttcatagt tatacgagct tttcaaaaga gataataatg
    26821 tcagacatat aaaaatgatg ttcttacacc gctcttagtt tttattcggt ttcacccggg
    26881 tttcctgcaa tttgagcctg ttcagcattt tgatcagttt ctgcgaattg acattgctgt
    26941 gaatgacgcc atccacctcc actacaggcg attgtgcgca ttgcccgaaa caggctaccg
    27001 ttttgatggt gaataaatta tcgggagttg tgtaagatga ttctgtatcg ttttcatctt
    27061 ttttgaaatt cagaattggt cctatttcat ccagcaaagc ttttgaacct tttgtatgac
    27121 aggcagtgcc ccggcaaacg acaatggaat gttttccctg aggtttcaga ttaaaaaacg
    27181 agtagaatgt gacaacacta tatacctggg tatatggcac tttcagtttt tgggatacag
    27241 cagtaagagt ttcaacggga agatatttca aaggattttt ttcctgcact tcttccagcg
    27301 ttgaaagcag tatgccgggt tttcctttgt gtttcccgat aatctgatcg agaatatttt
    27361 ctggaacagg agaattggtc agcgtttcca taacagttaa attatatgtg ttatttaaat
    27421 gaaaacatat tcttttaaaa ggatgaaatg actttttatt aaaaaaaatg ccacaatcct
    27481 gtttcctaca aaaaaactta tcaataactc tataatatat aactataaat aaaatagata
    27541 gttttagaat taaagaaata tttctttaat taattgcaaa tatttcaata caagcctgac
    27601 ttctgatgat aaaaaaagcg aaactttaca tttcgcttta caataatcac cgatacatct
    27661 tcgtcagtga taataaatct tataaatatt gttatcttaa ttcaatactt cgccaaataa
    27721 ggttgcagag ctggcatcag taattttcac acggacagtt tgacctattt tgcggccatt
    27781 acggggtata atcaccactt tattctgcga tgtccggccg aaaaattgtt cctttgaccg
    27841 ttttgagaaa ccttcaatca gtacttcaaa ttcgtgtccg atatctctca ggttgctagc
    27901 atgcgagagt tcgttttgta aagctatgat ttcgttcagt cgcctgatct taatttcctc
    27961 gggcacatca tccggcagat gcttggctgc atgcgtaccc ggtcgttcgg aatatttgaa
    28021 cataaaagcc atatcgaatc ccacttcgcg catgagcgac agcgtttcgg catgatcctc
    28081 ttcgctttca ccgtgaaaac cgcaaaatac atcggtacct atggaagcat caggcaaaat
    28141 gcgccggata gcagcaatcc tgtcaagata ccattcgcgg gtatatttac ggttcatagc
    28201 tttgagaatt ttattgctgc cgctctgcac aggcagatga atgaaccggc ataaattcgg
    28261 atgcgccgca atcacctcga gtgtatggtc gctcatgtct ttggggtgag aagttgtaaa
    28321 acggaaccgg atttcaggag ccgcctcagc cacaatttta agtaactgcg ggaaatcaat
    28381 aacctggcca tctttttcaa actggtatga atttacattc tgaccaagca gggtaacttc
    28441 tttgaagttt ttttcctgca gatctttcag ttcattcagg atgctttcaa catcgcggct
    28501 gcgctcacgc ccacgggtat agggcactat gcaataggaa caaaaattat tgcatccgcg
    28561 cattatggag atgaagcctg atatcggatt gctgatgcgg gaaggaatta catcacgata
    28621 ggtttcggtt tttgaaagct ctacattgat ggctttctct ccgttcatta catggcccat
    28681 caggttgggt atatccagat aagcatccgg ccctaccaca atatttactc cgtgattttc
    28741 aatcagatct tctttaaccc gttcagccat acaaccgatt actcctacta ccagtttttt
    28801 gtttttcttt ctcagcgact ggaaatactt cagacgctgt ataatttttt gctcggcatt
    28861 gtctctgatc gaacaagtat tcacaaaaac ggcatctgct tcagtaatct ggcctgttag
    28921 ttcaaatccg tccatctgca taatggacgc taccacttca ctgtcagcca cattcatctg
    28981 acaaccgtaa gtttcaataa atagtttttt gtcgttcaac gcggatttag agtccggcga
    29041 accaactgct ttgttttctt tcataaataa ataagtattt ttaaaatgtc tcacacaatt
    29101 tcccaatttt cggtgatttg ttttgtataa taatatagtt gtataatatc ttttttattc
    29161 gcatatccag cgtaaatctg catttattta tataacagca tcatcgtttt taaaatttcc
    29221 cagatgcaaa gttacgatat ttgtacgagg tgcagctttt cgaacacttt ttattttatc
    29281 gaatagctct ctgctgcacc cacaacgtga tttcaacaaa acgtataatc tgttcataat
    29341 tactcatttt tattcattta gaattaaatg aattattttt tgaaaaaaaa ttgttgtaat
    29401 atttggtaat tcaaaaaacc gtactaactt tgcagagtaa acaaacagat aaatttttca
    29461 taattaaggt ttaggttaaa atgtagcaat gggtgactgt gaagttaccc attgtttttt
    29521 tatgtaattt ggttgtacgg aattcttttt gtaatattgc ataaacattt aaaaatatat
    29581 ggaagatctg ggtgactata tttatcttat tttacttgtg cttgccggta taagcggaat
    29641 ttttaagaaa aaaggtaaaa gcaatgaaac cggagaaaag aaaaagccgg taattaacct
    29701 gcctaaatcg tgggaagagt ttgaacgaca gacaaccatg caaactccaa aaacaattac
    29761 ccgaccggaa ccatcccgta cgattatatc tccggcaaga acggctgtac cggaagcagt
    29821 atcgggcgaa acgattcccc ggggatttga aaccggaagt ttccagactt atgatgctgt
    29881 agatgatatt agcaaaatgc gggcaaagaa gaccgtaact aaaaatctga aatcacaaac
    29941 atataaccct gaacctgaaa tttcagaaga ggaaattaat cctttataca ttttgaataa
    30001 tgcagaaagt gccagagcag catttattta ctccgagatt ttcaacagga aataccatta
    30061 atttttttca aaaacttttt tcacaaaaaa attgaaatac gaaaaaagcg tgtatctttg
    30121 cagtaataaa acctgattca tactgagagt tccgactatt cgtcggaatt cttttttgtt
    30181 tatcaaaaac ttaaacttaa cacgattaat tatggcagta acgtatatga ccgctgaagg
    30241 ttataaaaaa ctagtcgatg aacttaatga aatggaaaac gtacagcgtc ccgccatttc
    30301 aaaacaaatt gctgaagcca gagataaagg agatttatct gagaatgccg aatatgatgc
    30361 ggcaaaagaa gctcagggaa tgcttgaaat gaaaattgct caactgaaag atattatcgg
    30421 atcagcccga atgattgacg aaacaaagat ttctacaaat gaagttcaga ttctgacaag
    30481 ggtaaaaatc aaaaatacaa aatccggtca ggttatgtca tatcttatcg tatctgaaag
    30541 cgaagccaac ttcaaagaag ggaaactatc agttaccaca ccaattgcaa aaggactgtt
    30601 aggcaaaaaa gttggtgaga aagccactgt catggttcct tcgggctcaa tggaatttga
    30661 ggtaatggaa attggactgt aaaatcaaga aaaacatcat cctgattgaa ttgtttattt
    30721 tgaaattttc ataggtctta aatcccccca aaaaagcatt tactaaccag aaataaaatc
    30781 aaccatcatg gcaacaattt tcagcagaat aattgcaggt gagattccga gctacaaagt
    30841 agctgaagac gaaaacttct atgcttttct ggacatcaac ccgatgagca agggacatac
    30901 cctggtagtg ccaaaaaaaa ctgaagacga ctaccttttc aatctggaca atgagactta
    30961 tacgggtctg acattgtttg ccaagaaagt ggcaaaagca attgaaaaat cggtggattg
    31021 taaacgcatc ggagttgctg ttatgggact ggaagtgcct cacactcata ttcacctgat
    31081 acccatcaac aaagaaagtg acatgaatat cggtaatccg aaactcaagc tttctcccgg
    31141 ggaaatgaat gaaattgccc aaaaaatcag aaataacttc ttgaactaaa cattcaacta
    31201 agaaaatccg ttatattttt tcacaaataa ataattacta aataatggaa acagtagaaa
    31261 aagtattaaa tgctatcaaa caagcaagcg aacctgtaaa tgcaggaaaa gtagtggaac
    31321 tcaccggact cgaccgcaaa gaagtcgaca aagccatgac aaagctgaaa gctacaggcg
    31381 atatcgtatc acccaaaaaa tgcttctgga cagcaaaata aattaagata cgagagcact
    31441 aagcgcatag aagttcactg tttttttcag ttgatatttt tatgaattgg tgctttcgca
    31501 ttttttataa ttaaaaataa tgatacaaaa aaacgacata ttccccatcg gacgactcac
    31561 gaaaactcac ggattaaaag gtgaaatgag tttcagttgt accacaacca ttctggagga
    31621 cattgaaatc ccgtatgttg tactcgaacc ggaaggaata cctgtgccgt tttacatcga
    31681 agagatacgg ttgaaaacag atgaatccgg aattatcaag ttcgaacgga tagactccga
    31741 agagcaggcg cgcgaatata ccgggctgac catcttcctg cccaatgaat atctgaatga
    31801 aattaatgaa gccggagctg atgcagatta ttttatcggt tttgaagtgt cagacaacga
    31861 aaaagggaaa atcggaacaa tcgccgggat tgaccaatcg actgaaaatg tactttttgt
    31921 aattgaaaca gaggacgatg aaatactaat tcccgctgtt gatgaataca tcaccgatat
    31981 tgaccacgag aaaaaaataa tccgcctgag tttacccgac ggattacttg atttataact
    32041 ttcgatgcgc taatcagttt ttttcacctc atttcgtaca ccttttctct gacaacagct
    32101 gcccactccg caggtgtttt cgtgctgtcg aaggtttgat atggtatcga cttacctatt
    32161 ttcactttaa aatgattgcc acgctgtttg tacatttcat tcaccagata aagcatttct
    32221 atatttggca aaccaaggcg ggttctgatt tttgacaaat tgtaaaagaa gccggaatta
    32281 cgaccctcaa aataaatagg aaccacatcc cgctgatact gaacagcctt agttacaaaa
    32341 gattttttcc agggaagatc ctgtattttt ccgccgattt tgcgggaaca cgtccctgct
    32401 ggaaaagtaa tcagatgaaa gtcggagctg aaaaattcat ccacaaccct tgcattcacc
    32461 cggctgttcg ccgaccctgc gcccacttta ttgaccggta aaaacatttc tttcagcggt
    32521 tccagaaaca tcaggatatc gttggcaaaa aatttgactt tcccgtcgta tttgcgtccc
    32581 agcatcaaac ctagcgtaac accgtcgaga ccgccgagcg gatgattgct tacaaagata
    32641 taacgtccat cctttggcgg caagttttcc acgccctcaa agtcggcacc ggcattcaga
    32701 tgttcataaa tggcagcttc gataaactcc agattttttt tatccggaac agacatgaag
    32761 aagcggttca attcatcctg atgaacaatc tttttgaggt aattaaccac gaacgcagga
    32821 aattttttat tcggagcttt ctcgcgaata atatcttcca cagacaatcg catcggtttt
    32881 atgctttcca tatttgttta cttttttcgt ttcaaactca caaaagtata aaaaaaacct
    32941 tcattctgaa atttatcagc tccggtcaaa caaatcgatt agtaatttgc acgcgaaata
    33001 tcaattgcag gtcggtggca taccatgaag aatacaaaca catcccccca ctatcactcc
    33061 actttcatat atcttcacac catcaatgtt atatttatct gatttcctta gttttatcta
    33121 aattaaatta attctctttc ccgggaaaca ttgttgataa attgttcaga aaacaggaca
    33181 aatcggttca taacttgttg ataaatattt tgtggggaaa tgtggagtat taggaaaaaa
    33241 tttcatactt ttaccatcca aacatatact caaaaaaaac atgtcaacat ttattggcaa
    33301 atatgacgca aaagccgatg taaagggaag aatcttcatt ccttcctgtt atcgcaaact
    33361 tctgtctgaa ggtgagcgtg aaaagctggt aatgcgaaag gatccggaca atgactgcct
    33421 gatactttat ccggaatcta tctggaataa aaaagttgac gattttaaat ccagactgga
    33481 tgactggaat gctgaagacc agatgctgtt aatgcagttt gtatcggacg ccgaatggct
    33541 cgatatcgat tcgcagggaa gagtacttat tacaaagaaa tacctgcaac taattggtat
    33601 tgaaaataat gaagtgctat ttgtcggatc gatggaccgg tttactgttt gggggaaatc
    33661 caggttcgaa caggcaaaac gttcaccggc agaatttgca tcccgactga aagataaaat
    33721 gactaaaaaa gctgaataaa tgccggaaaa tccatatcac atacccgctc tgcttaccga
    33781 gacgattgat ggactgaaca ttaacccgga tggcatttat gtggatgtta ctttcggagg
    33841 tggcggacat tccggagaga ttctgtcgcg tctgagcaaa aaaggaaaac tgatcggctt
    33901 tgatcaggac gaggatgtaa tgaagaatat cattgatgat cagcggttta tttttgtgaa
    33961 aagtaatttt cgttatctga caaatttttt gaaataccat cagatagagc atgtggacgg
    34021 cattctggca gatcttggcg tatcatttca tcatttcgac gaatcagatc gtgggttctc
    34081 attccgttat gacgggaaac tggatatgcg gatgaacaaa ctttcaccaa taactgccgc
    34141 cactatttta aatacttacg aggaagacaa gctcgcaaat gtattttatc tgtacggaga
    34201 gttacgcaat tcaagaaaaa tagcggcgac cgtcgtaaaa gcacgcgcac aggcacctat
    34261 cacacaaatt caggaatttg tcgagatact gaaaccattc ttcttcaggg aaaaggagaa
    34321 aaaagatatg gcacgcgtct ttcaggcatt gcgcatcgaa gtaaacaaag aaatgaaggt
    34381 acttcaggaa ttgctgaatc agagcgttga attactcaat ccgggcggaa gactggtcgt
    34441 actcacctat cattcgctgg aagacaggct ggtgaaaaac tttatacgca gcggtaattt
    34501 tgaaggagaa cttcaaaaag atttttatgg gaatgtgctt tcgcctctga aggctgtaaa
    34561 taacaaagta attgtggcat cggacgaaga aattgcccaa aatccacgcg caagaagtgc
    34621 aaaactcaga atagctgaaa aacaataata tgaactggct aaagaaaata tcagatacaa
    34681 tcaagggcag tgaagacttt tcggatatca aatcgagtac actcagagat attctgaatg
    34741 gaaatattct gaaaaagaag ttcatacaaa aacattatgc actttttata ctggtagcat
    34801 ttctggcttt tatatacatt gataatcgtt tttattgtga aacacaggtt tcacgcgaag
    34861 tgaagctgaa acagcaactg aaagatatta aatacgaatc gctaaccata tcggcacagc
    34921 tcacaacgct cagccgccgt acctatgtga tgaattttat caatgaaaaa ggattaaacc
    34981 tgaaggaaag cccttatgcg ccagtgatta tcactgaacc ggacccggaa aaagatcagg
    35041 agttgctctt acaaaaaaaa gaggaacagg aagctacaaa gaaaattgaa aacgacacaa
    35101 ctatcgttga agaatacata attaactaat tcaacatcaa caatgtctga tcccggaagg
    35161 aatataataa gcagatttgc agcagtttac atttttataa ttttactgat gctgctcgtt
    35221 atatataata ttgtagtgat tcagacagtg gaacgcaaag aatggctggc tttaggaact
    35281 aaaaataaaa aaacggacat catcgtgcag ccgaaccggg gaaacatcta ctcgtccgat
    35341 ggcagactga tggcaagttc tatacctact tattacgtct tcatggacct ccgtgtgcct
    35401 gcattgaagg aaaaagatgg gaaattattc aaagaaaata tcgactctct ttcgctttgc
    35461 ctggccgatt acttcaaaga caaaactcag gctgaataca agcgggatat taaatcagct
    35521 taccggaaag gaaaaggtga atatgctttc tataaaggaa aaataactta tgctcaactg
    35581 aaagatataa agaaatttcc gcttttcaga ctgggcaaga ataaaagcgg attgctgaca
    35641 aaagaaatgt tccgccgtgt aaaacctttt accacgctgg cttcgcgcac catcggcgat
    35701 atttatgccg atgaaacaaa aggcgggaaa aacggtctgg agcttagttt cgattcaata
    35761 ctcagaggct ctccgggtgt ttcgtcaaga caaaaagtag caaaccgctg ggaagaagtt
    35821 gttcaggttt ccccggttga cggcatggat atcaccacca caatcgatgt ggatatgcag
    35881 gatattgccg aaaaagccct gattgataaa ctgaaagaaa ttgaagctca gacaggttat
    35941 gccattctga tggaagtatc aacaggagaa atcaaggcca tagtgaatat gcaacggaac
    36001 gctgacggaa gttatacaga aaacaagaat ggagctgtgt cagataaggt tgaaccgggt
    36061 tcaacattta aaaccgtatc gctaatggct gtactggacg acggtaaagc aaaactcagc
    36121 gatgtaattg caaccggaaa tggtatttac gctttcggaa gagcaattat gaaagatcac
    36181 aacgcaaaaa aaggtggtta tcatgacatt acacttgaac aatcgctgaa tgcttcatcc
    36241 aatataggta tctcaagaac aatagtaaat gcttacggca acaatccggc tgcatttgtc
    36301 aacaagctgt ataagatgaa actgaacgaa ccattcaatt tatacattcc gggtacagcc
    36361 gcgccaaaca tacgtcatcc aaacgacaag tcaagttact ggtcactaag caccctgcca
    36421 tggatgtcca tcggttacga agttcaaata cctccaatat ataccgttgc attttacaac
    36481 gcaatagcca ataacggtaa atacattgag ccactgtttg taaaatcgat cagtaaaaac
    36541 ggacaaatca tcaaacaatt tacttcccga atcatcaacg aacgcatttg caaaccttcg
    36601 acgcttaagg atgtacgtca ggctttactg ggtgtagtcg aaaatccaaa atacggaacc
    36661 ggcaaagccg tacattctcc ctatgtgcgg atagcaggca aaacgggcac tgcacagatt
    36721 tcaaaaggat cacagggtta caaatccggt ggaacgacgc atcaggtttc attttgcgga
    36781 ttttttccgt tcgacaagcc aaaatatacc tgcctggtcg tgattcgtga accaaaaatt
    36841 ggctatcctt caggtgggct gatggcaggt gtggtggtaa aaaacattgc tgaacggatt
    36901 tcggctattg acaccgttgc cactgttcaa accatgcctg tagacagtaa ttatctggat
    36961 atcaagaaac ctgttttaaa atcgggtaaa ctggatgcta taaatacagt aatgaccaat
    37021 ctgaaccaaa agtacacagg caaaaaaagc gactgggtta aaaccacaag cgacggtaaa
    37081 accgttcatg taaacgaact tagcattaac aacaatacag tacccgacct gaccggtatg
    37141 ggcgcaaaag atgctgtata tctgtgtgag cgactgggac ttaaagtaaa ctttacagga
    37201 ttgggaaaag taatttctca aagccttaca ccaggaaaaa cagcttacag agggcagtcc
    37261 ataactttaa cattcaaata aatcaaccaa cttaataaaa atcagcatga atttagcaga
    37321 tttattacaa gatatattca tcgagcagat tacgggtaat tcagatattg aaataaccgg
    37381 aatacagttc gactcccgcc tggtagaaaa aggcaatatg tttgttgcta cccggggaac
    37441 cgcttcagat ggtcacaatt ttattcaaat ggctgttgag aaaggtgcag ttgctgttgt
    37501 ttgtgaagaa ttaccagctg agttaagcga cagaataact tgtattcagg ttaaaaacag
    37561 tgctgacgca ctcggtcacc ttgcaacgtg ctggtacggc tttccatcat caaaaatgac
    37621 gctggtaggt gttaccggaa ccaatggcaa aacaacaatt gcaaccctgc tttataaact
    37681 tttccgtaaa ctgggctatg gatcaggctt actttcgacc gtgtgcaatt atatcaatga
    37741 cgatgccgtt gaagctaccc atactactcc cgatgctctg gagctaaatg ctttactggc
    37801 taaaatggtg gatgcgggtt gtaaatatgc tttcatggaa gtgagttcac attctgtgga
    37861 acaaagacgg atagcgggtc ttgagttcga tggagctatt ttcacaaatc tgacaaggga
    37921 tcacatcgat tatcacctga cttttgaaaa ttacctgaaa gcaaaaaaac gattcttcga
    37981 cgatctggat acaaatgctt ttgcacttac caacatcgat gataaaaacg gatcagtaat
    38041 gcttcagaat acccgggcaa agaagttcgg ctattcaata cgcaccatgg ccgattttaa
    38101 aacaaaaatt ctggaagacg gatttgacgg catgctgctt gatatgaata atcatgaagt
    38161 acatgtttca ttcatcggca aattcaatgc ttataatctt tcggcggtat ttggcgctgc
    38221 tgtattgctc ggccaggacg aaatggaagt actccgcatc ataagttccc ttcagccagt
    38281 ttcgggcagg tttgaaacct tgtactcgcc caccggttat gctgcaatcg ttgattatgc
    38341 acatacaccc gatgccctga ataatgtaat atcaactatc aatgaaatat tgcgtggtaa
    38401 aggtcgatta attaccgtag taggatgcgg tggtaaccgc gacaaaggaa aacgccccat
    38461 gatggcacgc gaagctgtcg atggaagctg gaaagcaatt cttacttccg ataatccccg
    38521 taatgaagac ccgcaggata tacttaacga catgctcgat ggtctggatg cttcccagaa
    38581 atcaagatca ctaacgatag ttgaccgcag ggaagctata aagactgcct gttcaatagc
    38641 tcaaaaaggg gatgtagttc ttgttgccgg aaagggacac gaagattatc aaatcattca
    38701 gggtgtaaaa catcattttg atgacaaaga agaagtgaga aaatgttttc aataatcaaa
    38761 acacactcct tcagataaat tacagactat tgtccggata aggacattaa ttatgaattg
    38821 taaataatta aatttaagct tatgctgtat cacttaatcc attacttaga caaatactac
    38881 gacatacccg gttcgggaat gtttctgtct atatcatttc gcacaggtct ggcttttatt
    38941 atggctttgc ttttatcgac catgattggt cgaaaaatta ttgacaaatt acaaaaactt
    39001 cagattggag aaacaattcg tgatctgggt atggaagacc agttacaaaa gaaagggact
    39061 ccaacaatgg gaggtatcat tattatcata tccatattaa taccaacttt attattggct
    39121 gatcttacaa atatctacac acttttaatg ataattacct gcgtgtggat gggagctatt
    39181 ggatttctcg atgactttct gaagataaaa tacaaaaaca aggaagggct taaaggaaat
    39241 tacaaaatag ttgggcaggt tgggcttggt ctcattgtgg gactcacact ttatctgagt
    39301 cctgatgccg ttattcgtga aaacgctgaa ataaaaggga aaagcgcccg cgtagaagat
    39361 gtacgatatg cctcgcagga tgtaaaatcc accaaaacaa ctattccatt catgaaaaac
    39421 aataatcttg attatgccga tgcctttaaa tggggcggtg ataatgcaca gaaatatggt
    39481 tggatatttt ttgtattgat ggccattttt attgttactg cggtttccaa cagcgccaat
    39541 ctcaccgatg gcatggatgg gcttgccact ggctcttcgg ctataattgg agttacactc
    39601 ggcattttag cttatgtttc gggtaatgtg aactatgcag gctatctgaa tatcatgtat
    39661 ataccgggta caggtgaatt gctgattttt gccggttcgc ttattggtgc cacagttgga
    39721 ttcttatggt acaattcgta tcctgcgcaa gtgtttatgg gcgataccgg aagtctgact
    39781 ctcggtggaa ttattgctac tttttctatc gcaatccata aagagttgtt aatcccaatt
    39841 ctgtgcggac tatttttcgt ggagagtatt tctgtaattg ttcaacggtt ttatttcaaa
    39901 tatacaaaaa aacgttttgg agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40021 nnnggtgaaa gcggtgctgg cgcagctgtg ttggggaaaa aacagggata tgatgttttt
    40081 gtttcggata tgggtgagat aaaaccggaa tacaagaagt tgcttgatga tcatgaaatt
    40141 aaatgggagg aaaaaaagca tactgaagag ttgattctgc aagccgatga agtaataaaa
    40201 agccccggaa tacctgataa agctcctatc attcagaaac tgaaagctca gcatactccg
    40261 attatttcag aaattgaatt tgctggtaga tataccgatg caaaaatgat atgcatcacc
    40321 ggtagtaacg gcaagaccac tactaccatg ctgatttatc atattctgaa aaacagcgga
    40381 ttgaacgttg gtctggcagg caatgtggga aatagtctgg cactgcaggt agctgaaaac
    40441 agttatgatt attacgtatt ggaactcagc agttttcaac tcgacgggat gaccgaattc
    40501 aaggccgatg ttgccatact tctcaatatc acacccgacc acctggacag atacaattat
    40561 gaattccaga attatattga ttcaaaattc agaataatac aaaatcagac tgaaaaagat
    40621 gcttttatct tctggaacga cgatccggta atccgcgaag aacttaagaa aagagacata
    40681 catgctacat tgtttccctt ttcactccaa cgtgaggaaa aaacaaaggc atttatcgaa
    40741 aacgaacaat taatcattaa aacactaaat accattttca gtatgcctgc aaacgaactt
    40801 tcactcaaag gtatgcacaa cacgttcaat tcgatggcag caggacttgc agcttccatt
    40861 gtcaatatcc gcaaagaaaa catcagacaa tcattacagg attttcaggg agtggaacac
    40921 cggctggaat atgttgctac cgttaaaaac ataaaattca taaatgactc gaaagctacg
    40981 aatgtaaact cctgctggta cgctcttcaa agcatgaaaa cacctgtaat actcattctc
    41041 ggaggtacag acaaaggaaa tgattatacg gaaatagaag aactggtaag agaaaaagtt
    41101 agtggactga tttttctagg agttgataat tcgaaacttc ataatttctt tgatggaaaa
    41161 attgaaagaa taaaggatgt gaactccatg gaagaatgcg tcaaggaagc ttacaacatg
    41221 gcaaaacctg gcgatacggt gcttttatca ccttgctgtg cgagcttcga tctatttaaa
    41281 aattacgaag accgcgggaa acaattcaaa atctgtgtaa gaaatctgta agaattagat
    41341 aatcttacag cattaatctg aacaacaatt caccaaattg attataaata acagtggaaa
    41401 atttatttaa aaaatatctg aaaggagaca ccatcgtctg gacggttttc tttattctgt
    41461 gtatattttc tatagttgaa ttgtacagcg catcaagcac gcttgctttt aaggcagcca
    41521 atcataccgc accaattatt caacatttct cgtacttatt tgtaggcgct gtcgttgcat
    41581 ttcttgttca cttagtgcca taccggtata ttcgtatttt agctttcttc ggacttttca
    41641 tttccattct gctattgatt tatgtgctgc ttaaaggtcc tgacaccaat gatgccagca
    41701 gatggatcag gttattcgga atccagtttc agccttccga aatagcaaaa ttatccttga
    41761 ttgtggttgt tgctgacttc atctcgcgta tcagggcaaa tcctgcagat gaaaaaagaa
    41821 atttctggat aatcatgctt gtagcaggca taatttgtgg attgatactg cttgaaaact
    41881 tctccacagc agctattttg ggccttacca tctacttcat gatgttcatt ggtcgtattt
    41941 cgtggcaaaa acttgcatct atattgggtg ttggagttat tctgcttatt ttaggttatg
    42001 ggttggtaaa aaccgttccg gaaaaaaaaa tgccgaaaat gttccaccga gcttatacct
    42061 gggtagcccg tgtagacaga tcggctgagg aaaacgataa agataaatat gtaattaatg
    42121 atgaaaatct tcaggtacag catggtcgta ttgcagttgc acgcggagga tttttcggag
    42181 tatttcccgg caacagtgta caacgcgact ttttaccaca tgctttcgat gattttatct
    42241 tctcaattat catagaagaa atgggcttat tgggaggaat ttttgtcatg tttctatacc
    42301 tggtactcct cttcaggaca gggcaaattg ccacagtatg caaaagcact ttcccggcaa
    42361 tgcttgttac gggtcttggt ttactgatta ccctgcaggc aactgtcaat atgtttgtgg
    42421 gttccggttt tggtttggtc acgggtcagc ctctgccatt aataagcagg ggtggcacat
    42481 ccattctgat aaattgtgtt tatttcggca ttatactggg tgtaatacgc gaaataaaag
    42541 ctgaaaatga gaaaaaaacc aaacaaaaag atgatattga taaatcccaa caggaatctg
    42601 atacggaaga agattatgtg ctggacatcg aagaaatctg attannnnnn nnnnnnnnnn
    42661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42721 nnnnnnnnnn nnnnnnnnnn nnnngttcgc ggtttcgacc gtaagaatct actgaagaat
    42781 gtgagcgtaa ttatagattt gctgaaaagt acacgcaaag caaaaaaaat cataaaaaat
    42841 tttgctccgg atattgccat tggtgtaggc ggatatgcca gcgcgccgac attaaaagca
    42901 gcttcgggat acggagtgcc aacgcttctt caggaacaaa actcgtatgc aggagttacc
    42961 aataaacttc tcgccgccaa agcaagcaaa atatgcgtgg cttatgaagg aatggaaaaa
    43021 ttctttccgg ctgataaaat aatacttact ggtaatcctg tacgtcagga tttgtttgat
    43081 gttgctacca aagcaaaaga agcgtatgaa tatttcggtt ttagtccgac caggaaaacc
    43141 attctgatcg tcggaggtag ccttggagca cgaaccatca atcttagtat aaaaaacggt
    43201 ctggataaac tcaccgaaaa ggatgttcag atcatctggc aaaccggaaa actgtacgca
    43261 gcggatgccc gggcggcagc ttcaacgtat cagactgaaa aactggtggt gactgatttt
    43321 gtttcgcgca tggattacgc atattccatt gctgatctgg ttatttcacg ggcaggtgcc
    43381 agttccattt cagaactttg cctgctggca aaaccggtta ttcttgttcc ttcgcccaac
    43441 gtcgccgaag accatcaaac taagaatgca ctcgcactgg taaataaaga tgctgcaata
    43501 atgattcgcg acatggatgc ttcaaatgaa cttgtaccga aaacgcttga actaattagt
    43561 gacaatccta ccctggagcg tctatcaaaa aatattctac aacttgcatt acctaattcg
    43621 gctgacagaa ttgccgaaga agtttttaag attatcaaaa aaaagtaatt aacattgact
    43681 atgacctata caagatatta tttcattgga attggtggaa ttgggatgag cgctattgca
    43741 agatacttcc atgtgaaagg atttaaggtt gcaggatacg atcggacaga aactaaacta
    43801 acttccgatc ttcaggctga gggaatacag attactttcg aagaagctgt cagcaccata
    43861 ccgtccgatt ttctggaaaa agaaaaaaca ctgattgtga taactccggc aattccgcaa
    43921 gatcacgagc agttggtgta ttttaaggaa cataatttca ccatcatgaa acgttccgaa
    43981 atgctgggcg aaataacccg tcaaagcaaa gctatttgca tcgccggaac tcatggtaaa
    44041 accacgacct cgacaatcac agctcacctg ctataccagt cgcatgtgaa ttgcagcgcc
    44101 tttctgggag gaatttccaa taattacaat accaatctgc tcctgtcaaa tgacagcaac
    44161 ttcgtagttg tggaagctga tgaatacgac cgctcttttc accggttaac tccatatatg
    44221 gctgtcatca tttcggtcga tgctgatcat ctggatattt acggatcgga ggaggcattt
    44281 ctcgaaagtt ttgagcattt tacatctctc attgctccgg gtggcacttt ggttatcaac
    44341 aaaaatgtac atattacacc aaaactgaaa aaaggagtca ggatgtttac ctacggactg
    44401 aactcatcag ctgatttcta tgctaaaaac atccgaatgg aaaaaggcaa tatcctgttc
    44461 gattttgtaa ctccttcaga tacaatatat gacgtacggc tgggtgtgcc ggtaatgatc
    44521 aatgttgaaa acagcgttgc agccatggct atggcctggc tcaatggtgt gactaatgaa
    44581 gaaatccgaa ccggaatttc aacattttca ggaatttatc gcaggttcaa tatcgtttat
    44641 aaaacagata aaattgtata tatggatgat tatgcacatc atccaagcga actcaaagag
    44701 agtattagtt cgatcagaca actttacccc ggccgaaaaa ttacaggcat atttcaacct
    44761 catctttaca gccgcaccaa agattttgcc acagaatttg ctcagtcatt gagcaatctc
    44821 gacgacgtaa ttctgcttcc tgtttatcct gcccgtgaac tgcccatcga aggagtaagt
    44881 tctgacttaa ttctaaaaga tgttactgct cagcataaaa cattatcgac taaagaaaat
    44941 ttactaactt tgttagatga aaaaaatctg gatgttttag tcacttttgg cgcaggagac
    45001 atcgacaaac tggttccgga aataaaaaaa tacctgaaaa aaagatataa ataaaacacc
    45061 tataaaattt tcaggttagt attggttaaa aacgaattgt tacaatataa aatgaacaaa
    45121 attctgaaat atatattgct ttctgtggca gtcttgctgt gtttgggata tgcagtattt
    45181 gctgctgcca atggcaatac tactgcaaaa gaacaggttt gctcagctct taaagtacat
    45241 attaacgatt ttgatcacag gcaattgatt tccaatacag aaattgcaaa attactagag
    45301 tcggaaggac tgaatccgat gggtaaaacg ctaaagagga ttaaaacaaa atcaatagaa
    45361 gacgccattg aaaagcatcc gatggtgaga caggcggaat gttataaaac acccgggggc
    45421 gaaattgttg tcaacattga acaacgaatt ccggtacttc gtgttgttgg atttgagaat
    45481 tactacgtgg atgatttgcg aaagcctatg cccgtttctc agaattttgc agcctatgta
    45541 cctgtggctt cgggcagagt aaccagaaaa atggcaaccg gctccttgtt tgatttctgc
    45601 gagtacatca acaaagatcc attctggaat aatcagattg agcaaattca tatcaatgac
    45661 aacctggatg tagaacttgt acctcgcgtt ggagatcata ttgtgatgat gggagatttc
    45721 agtcgttatc agcaaaagct tgagaaactg aaaaagttgt atctttacgg attgaatcaa
    45781 atcggttgga atcagtataa aaccattgac cttcagttca aagatcaggt tgtttgtaca
    45841 aaaaaataaa ttatctcctc ttcaaaaata tctgttatga gtaaaaacac aattgtacac
    45901 attgatttgg gaggttcaca cataactgct cttgcaggca ttgtgctgga tagcggtgaa
    45961 ctaaaaattt taggcgaaca gacacgtgca agcgatgatg ttaaaagtgg aattgtggaa
    46021 aaaatatcgg gcgctgctta taaaatcaac gaaacgacaa aactccttca aaatgcactg
    46081 cgcattgatc cgattaccag tgtgagcatc actgttaatg ccaaatcaat gaaacaaatc
    46141 ccttataccc tgaaggctaa tatcagaaaa cctgtcactg aggggatgtt gaaggaactc
    46201 gaaaaagaat gcaaacagga aataaaaaat gacaagacaa atgtaattga atgcattcct
    46261 ttggcatatt acatggatgg caagcgaatt gccgatccga ttggacaaaa aggatacaac
    46321 cttcgtaccg attataatct tattgtctgc aattcgctgg tgaccgaaac gattgaacga
    46381 agcatagagc gtacgggcat tgccgtggat tatattcatc tggggatgga agcgatagct
    46441 actgcggtac ttgaagaaga cgaccgcgaa aaaggctgtg ctatcatttc ctttggctcc
    46501 acgaccacca cgttagctgt atatcacgaa ggagtgttgc aggaactgat ggttgttccg
    46561 tttgggggac ataacattac caaagatatt gaagaactcg gcataagttt ttccaacgcc
    46621 gaacttctga aatgcaaaac aggcagtgcc atggaagaat tgatcaccaa gcctgtaaac
    46681 gttcaaattc ccaatgtcga tccaacaagg gaaccgatac tgatctcaac caaattcctt
    46741 tccaccatta tcaatgcgag acttgacgaa atactcgaac ccattctcgg acaaattgat
    46801 cagatagcat atccgctgaa agccggaata gtaattaccg gtggagcatc caaactaaag
    46861 aatctgaacg aatatctgac aaaaagaacc ggattacagg ttcgtacggg aagtcatgca
    46921 gaatggctct catatgatac agatccaaaa tataaagatc ctgtttactc ccaggctatt
    46981 ggtgcaatac tgcttaccga tgacaagaaa aaacagaaag gggaagaaga aaaggttatc
    47041 caatcaagaa taccttccaa aaacatattt acaaaggctg gcttgaaatt taaagaaaaa
    47101 atgaacgacc tttttgatta tgacgacata gaacaagaaa aaaacaatca ataggttatt
    47161 aattgaattt taacatatta acaagatctg acagcttttg cataacgaaa aacaatgcaa
    47221 gctctggcag taaaacaaca ataaattatg gattttttag aagataccct tccactggac
    47281 ttacctcttg caaattcgtc cattattaag gtaatcggag ttggaggtgg cggcggaaat
    47341 gctgtgaatc atatgtacaa acagggaatt gcggatgtat cattcgtggt ttgcaataca
    47401 gataatcagg atcttgtgcg atcgcccgta cctattaaag ttcagctcgg gaaaagcatt
    47461 acacagggtc ttggagcagg cggccgaccc gaaattgccc ggcaggctgc tgaagaatca
    47521 gtcgaagata ttcataaaat gcttgctgat aatacaaaaa tggtatttat cacggctggc
    47581 atgggcggag gcacaggtac cggtgcttcg cctattgttg caaaaacagc acacgacatg
    47641 ggtattctca ccgtgggaat tgtcaccatt ccgttcgcat tcgaaggaaa cctgaaaata
    47701 cggcaggctc tcgaaggtgt ggttgctctg agcgaatatg tggatgctat cctggtaatc
    47761 aacaacgaaa aactgaagaa tatatatccc gatttgaacc tgtcgaatgc ttttgccaaa
    47821 gctgatgatg tactgaccaa cgctgcaaaa gcaattgccg aaattattac catctcggga
    47881 tatatcaata tcgattttgc ggatgtgagc acaatcatgc gcgacgggaa agttgcaatc
    47941 atgaataccg gctatgcttc gggtgaaaac cgtataacaa gagccatcga ggatgctctc
    48001 acctcgcctt tgctgaatac caacgatgtg aaaggtgcaa aaaaagtgtt gcttagcctc
    48061 tattgttcgc acagcaatga aataaaaatg gaagaaatta cccaaataca cgagtttatg
    48121 gctaaagttg gagagaacgt taccgtaatt tggggtgcaa gctttgacga cgaactgggt
    48181 gatgaagtga aaatgacact gattgctaca ggttttgacg taaaagacat tccgggaatg
    48241 ccttccggct tgataaaaaa gagaaaaaca gttgaagaga aaaaagaaaa cgctattgaa
    48301 gttatagaag aacctgtttc ggaagaagaa ctcgaattca acaaaaaatc cagcgaagag
    48361 gaatttaata aggcaatggg cgaactctat ggtccgtctc tccgacaaat aaaaacagag
    48421 aagaaggaag aagtaatcca ggaaatccta caatttgaag cagctccgga agataaactg
    48481 ccggtagtca acatctttga gttggaaagt gaagaagatt taaatcaggt tgaacaagtc
    48541 ccggcctgga aacgaagatt tatgaaaaaa tagggttatc aaacatcctt ctgaaaaaaa
    48601 tagaaatcaa tcaaacaaca agatggaaga aaaaatattt gaacaaatca gcgctgacat
    48661 aaaaacagcc atgttagcac gcgataaagt gtcactcgaa gcactgcgcg gcataaaaaa
    48721 agaattcatc gaagccaaga cagcaaaagg tagtgacggt gttctgcacg acgatcaggc
    48781 agtgaaaatc atgcagaaaa tggtaaaaca gcgcaaagaa accgctcaga tttataagga
    48841 acaaagccga cccgaactgg ccgaaaatga actgtccgaa gccgctgtca tcgaaaaata
    48901 tctgccacag cagatgagcg acagtgaact ggaagcagca gtatcgaata tcattgcaca
    48961 aattggcgcc gctggtccac aggatatggg taaagtaatg ggaatagcat ctaaacaact
    49021 ggcaggtaaa acagaaggaa gacttatttc ggaaaaagta aaagctttac tgaataaata
    49081 aatacccgct ctctgctata aaaataaaat ctcctggagt aaattactac aggagatttt
    49141 ttattataaa acagttgcgt ttctatttaa tttcaagtac tatgattgat ttttcaggca
    49201 aattaacgtt cagaactccg tctgtaaatt tgaattttgt aaatgcttca ggttttactt
    49261 tatcgggatt ttcaaaggta ttgtggtcct gtacggtttt tgaggtcaga attttccctg
    49321 taacctttgc tgcctttttt cctgcaagat caattttcac ctttttagca ttgtccaggt
    49381 cggcatttgt gagtgtaatg tgtataagtc catccttctg cgatgccgaa gctgtcacca
    49441 ttttcagttc cttattattt acggtttttg tatcggaatt aatttcaagg ggcagatacg
    49501 ttgcatcctg atgaactttg tacatgtcaa aaatataata tgttggggta agcaccattt
    49561 tatcatcttt ggtaagtatc atcgattgaa gcacattaac aatctgcgcg atgtttgcca
    49621 tacgtaccct gtccgaatac ttattgaaaa tattcaaact gatagctgct acaaatgcat
    49681 cccggagagt attctgctga aacagaaaac ccggattagt tcccggttcc acatcgaacc
    49741 aggttcccca ttcatcaacc aacaaaccga tcttcttttt tgggtcatat ttatccatcg
    49801 taccgatgtg tttgataagt acgtcctcaa tttctgagca ttttgcaagc gttttatagt
    49861 aatcatcacc attaaattgg gttgctgaac ctttgtcgtt ccagtctctg atggtataat
    49921 agtgcagcga gagtccgttc attctgtcac caacctgctt catcagcaca tcagtccagt
    49981 tataatcgta atcacttgca ccactggcta ttttataaag attatttccg tcgaaattgc
    50041 ggcaataagt tgaataacga cgatacaaat cagcataata ctcgggtctc atgttacctc
    50101 cacatcccca gctttcattt ccgacaccca gatatttcac attccagggt ttattccggc
    50161 catttgcacg acgaagattt gccatcgggc tatcgccatc ggaggtcatg tattccaccc
    50221 acttggccat ttcttcgact gtaccgcttc ccacattgcc cgaaatatac ggttcacatc
    50281 cgagcatttc gcataagtta agaaattcgt gagtaccgaa actgttatct tcaactactc
    50341 cgccccagtt gttattcacc attttcggac gtttttcacg gggtccaatt ccatcagtcc
    50401 agtgatattc gtcggcaaag catcctcccg gccatctcaa cactgggatt ttgaggtttt
    50461 tcagcgcgtt gagtacatcg ttccggtaac cgttggtatt cgggatcgaa gaaccctcac
    50521 caacccaaat cccgccataa atgcacgaac cgagatgctc cgagaattga ccgtagatat
    50581 tcttactgat aatctgattt ccctgttcgg gaagaatttt aatagtaata tcgccggagt
    50641 tttgaccgct aacagcaata ttcaaaaaaa gaaatgcaag cgttgcataa aaaaatcttt
    50701 tactcataag cttttttgaa ttacttaaat catttattat tacctgaatc aggcatacaa
    50761 tggaacgttt ttttctatgg tcaaaaataa tttttatcaa tggaaatgta attacacttt
    50821 ttgaaaaaga atagtcataa atgatattta ttcttcaaat tccgataaat attcatacgg
    50881 tttcacttaa taaaacgaac catttcacag gcagcgagca aaaaagctcc aacaccaaaa
    50941 tcggctgtag aacgggcatc aactatttgt ccgggtatgg ctttctcgcc aattggttga
    51001 acatatccta ccctgccatc aggctgcaaa gccactttgg taagataatt ccaggatttt
    51061 tctatcacgg gacgataggt ttttttatcc aacaagccat tattcatacc ccaaagtagt
    51121 ccgtaagtta aaaaagccgt tccgcttgtt tcggggccgg gagactggtt tttatccagc
    51181 atacttctgg tccagtagcc ttcgggttgc tgacagttgg caatagcccg agccatggtt
    51241 tggaatcgct gaatgtattg tttccggtat ttgtcattct ttggcaaatc attcagcact
    51301 ttagcataac ctgcaaaaac ccatccgttg ccacgcgccc agaagtcctt ttcaccacta
    51361 agtgttttat gtttggggta aacatatttg gcatcgcgaa aataaagccc cgactcagga
    51421 ttgtacatga tgctgtcgga aaatttcaga tattcgttca gtttttcgga atacagatta
    51481 tttccggtga tattgcgcag ttttgtcatt acgggcataa ccatataaag tccgtctgcc
    51541 caccaccaat aattattttg cgatgtactc atctggtatt ccattacttc acgtgcccgg
    51601 gctatttttt tgggttcggg tgaaattttg tacaaatcgg caaatacctg aaaacagatt
    51661 tgttgatcgc cgaataatac ataatcgtcc gtttcgccat aggtatattt ccatttcgtt
    51721 ttatcgtccg attttgcacc cttccattca ttaaattcag cccaatccag agaatattgg
    51781 agatactttt tatcttttgt cagaaaatag acttccatat tgccggtatg atatgcggca
    51841 ttatcccaaa atgctttctg ttgagccgga tggctacttt gccagtaatc atttacctga
    51901 tgaataatat ttattatccg gttttcttcc tgactgactt tttgaactgt acaaccggta
    51961 aataaggtaa taactattgc cactgaaaat aatcgtatgt gcttcataga taagtttatt
    52021 ttatgtttag aatataaaca ggctgagcgg taaggtcggt taacttttca ccatcaccct
    52081 gagcggtatt aatcacgaaa acttgcaggc ggtttttcgt tctccagagt tctgtatcgt
    52141 acgttggttc ccaggctcca acctgaaatc cggtcatatc cttgaaattc catattccct
    52201 gattgatatc cgaacagctt gccagtgtca ctttactgtc gcgttcagca tccctgtaaa
    52261 tgatttttat ttgattcttt ttatcaataa cgatacgggg acgagataca ggaatcattt
    52321 ttgtgccgcc cccgctcagg ctgaacggcg ttgagcggtt ggttacctgc tgttgtttcc
    52381 acttattccc gtcaaaccaa actacacgga actgaggtac ctgagtagtg ctgtcgcgcc
    52441 aataagtggc tatgtatgga ttgccattcg agtcagctgt catggatgtc tgatttataa
    52501 gctcgctctt ttgtggaatt ttccaggcat attcggcgct ttcagccgtt attggcaagg
    52561 tatatttttc accatttgat ttttcccagg ttttaccatt atccaacgac cgggcataac
    52621 acaaatcatg attggtttcc actccgggtg attcgcgcca aacccatgac aggtgtattg
    52681 ttcctgcatg atcgacaaac agttgccagt atgcatttct cctgttttgt ccatcaatga
    52741 gaatggagtg catattctcc caggtttttg ttttcaggtt gtaagttttc attaccatgt
    52801 tgccacgtcc cgaagcaccg gaacgataca caaacagcag atttttattt ctcaaccggt
    52861 aaaattcagg ataggtaact ttattttcgt cgttgccggt catctgctct ttatcgccca
    52921 ttttcagtga gtttggttgc aagctcctgc aataattgag cggtctgtcg tgctgatcga
    52981 aactcatatg cagatatcca tcatcgtcgg ccataatgct gatcacatta tgcgcgtcgg
    53041 cacagtttcc tgtaaatcgg gttttttcaa acttccattt tttttcggtg attttacgtt
    53101 gtcccagcac gataaaacca tcttggtcgt aaaatgcaat atattgatat tttccggaac
    53161 ttaccaccga gttggatctg aaaactgtgg tgtttacgga attacggctg tacgccatac
    53221 ctccggtatc catgagtttc tgagcttttc cggctgtgac agtgataata agaaaaagaa
    53281 ataataattt acgacctgac ataaattgtt ctccgatttt attatgctta atagtttacg
    53341 taataatctt tattgaatgt ttcgttccgg aatgcttttt tagctgtcca ggcttcttcg
    53401 ggtgctgtcc agaatgtgtc gctggcaggc aaaccaagtg tcagaaagct ttcggaagtg
    53461 atgtacatgc ttccgttatt gctgtaccag tctccaagcg ttggctgatg tcctgcaaat
    53521 ccgatcgtca gatatccgtc ggaagtaaaa ttagatgaat tattaaaaat gcttttatgt
    53581 acagctttta aagctgatct cacttgtcct tcggttaatt ttgccggcaa ttgcttacgc
    53641 caggcaagca aagccagggg ctggaaaaca gccgtccgat acgtcagcga ccttccaagg
    53701 ggcggatatg ttccttcggg cgaaatcatc cgttccagat gctctgaata acgctgtatt
    53761 cttttcaatg tctgttcgta agccgacttt tcagccttac catttttcac caacacttcc
    53821 atcacttcga gcatcatagg atgcatgacg taagaattat aaaaatccat gtgaaactta
    53881 tcgccatcag aatgccaacc gtcacctaca taccattcat tgattttctt tagtgcaaag
    53941 tcaatttttt ccgaatcata ctcctcgcct atcgaaagca ggaatgcttc gttcatggct
    54001 gcaaacaata gccagttggt aatgggtggt ttcactctgc gcaactgttt gagttcagta
    54061 actattctcg ccttggtaac actgtcaaga ggctcccaca attgttgggg agcacgcata
    54121 agcgcgttgg aaaaatacgc agaatcgacg agcggctgcc cctccttccg ccagagcaga
    54181 taatcaggac tttgcggatc gactgaatga gcataacttt tcaacgccca ttccttcatc
    54241 tgcctgcgac actttccttc ctccgtgttg tcatcgggca atgtcagcca gggcgccaga
    54301 ccagccatca accggccaaa acattccatg taagttactt tcatatcacg tccatcccag
    54361 ctcgggctca gttcaacggt catgtttttt tgcaactcac cgttcgacat attacgaagt
    54421 acaggttccg aaattttata aagcaatttc cagaaatatt cgcgatccgg aatgcttttc
    54481 tgcattttct gcttgttttt gatggcgctg aatgttgagc tttgagaaat ctgaggcagt
    54541 aaagttcctg cagtagtaac accaagaatt tttataaaat tgcgtcgtat catttaagta
    54601 tatttttaag tgcattttcc aaaccgggat aaacaaattc gaagccggca gcctgaattt
    54661 tttctgaaga aattcggctt ccttcaagca gtaaacctga catttctcca aaaataattt
    54721 tcaggaaaaa agcaggaata tttggcagcc aaaaaggttt atgaagcacc atggataatt
    54781 tcttcatgaa ctccttatta gtaatttgtt caggcgcaac ggcattataa actccggaca
    54841 tattattatc ttccagcgct ttgatataaa tactgcacag atcgctgata tgaatccacg
    54901 gcaggtattg tctgccacta ccaacagcag ctccgactcc tgctttgatc tgaaatatca
    54961 ttttcgataa ggcttcattt tgtttatcga ggactacagc cgtccggatt attacagtac
    55021 ggatatctgc tttttgaaaa ccggatgcaa cttcttccca ttggcggcaa gtggtgccgg
    55081 tgaaatcatc tgaaggtaaa tcggtttcaa tgaaaacttt atcggcagtc accattccgt
    55141 aataccctgt agccgatgcg gaaataaaag cctgaatgct tcgttttctt tcaattactt
    55201 tgtcgtaaag caatttagct gtcaataccc ggctatcaat tatctctttt ttcctttttg
    55261 cagtccatcg tttacttcca atattctcac ctgccagatg aataatgtaa tcagccgtat
    55321 caattgcttc atcttctaat tgctgatgtt ccggatccca taaaaaaact ttacaacccc
    55381 tggcgggttt gctgcttcgg cttaatactg aaacctcata acctctttcc agaagcttct
    55441 tgctgagatt tctgcc
//
LOCUS       DAOPYB010000002        52841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_484_length_52841_cov_9.004300, whole genome shotgun sequence.
ACCESSION   DAOPYB010000002 DAOPYB010000000
VERSION     DAOPYB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 52841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52841
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_484_length_52841_cov_9.004300"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..989
                     /locus_tag="PKH58_00240"
     CDS             222..989
                     /locus_tag="PKH58_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87481.1"
                     /translation="MPLGERETHPYLEQGQIIGATKLILGSFPVYDCTDNDNDLKKQN
                     RQNKGTVRFFYGSYRNSLWIKYSQFIDNTISKPWNTELILESLRKRKIAVSDTILSCE
                     RYKYKIDKKTGTKILFPYSSEDSALKNKNWNRVIIRTLINNGVTKILCTSKGVLNDLE
                     RQIICFGRNNYGRKDNQLSSLFQSQFIERVGGNNNQITNDIAKIFIVGNSQIIAIALP
                     SPGAPQRQTHEFGCNNQNRLEYSENYFQNAFNWLLND"
     gene            1105..1554
                     /locus_tag="PKH58_00245"
     CDS             1105..1554
                     /locus_tag="PKH58_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87482.1"
                     /translation="MKTIITIAFLLFTVNSFGQTRTYRLEDLTEFSKNHYGFLKTDKT
                     INEYYGDFDCIDKEKSIFNFNVGCGRGGDIISILIKENSSRLNCFDLYLQKGDIYYGN
                     QAYTNKKIGEFLIICEDEIRGNIKLCLEKGAACCGEEGEIFTLMKLK"
     gene            1752..2693
                     /locus_tag="PKH58_00250"
     CDS             1752..2693
                     /locus_tag="PKH58_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87483.1"
                     /translation="MTKTILTTFLLSICLTTCGQNKIENKLTEEHQNIRGTKVSLIPP
                     KGFTDGLNFLGLQQTESGSSIMILDIPGPYSETSAGINKENLLSKGVELSKIEKLTIN
                     GLPAIFVTGTQNAYGNIYTKFILVFGTENETIMINGVHPENLKKLGDEIRKSMLTVFY
                     EADKKINQFDALDYTIDVSQTKLNFGKSMSNSLIFSVDGQVPTASSDKTNLIVAKSFS
                     QITTEDKKLFCINRLKQTPIEIVNIEYTNEITIDGVSGYEIYAKGKSKKTGDTDNIYQ
                     VILFSDKLYYILFGTTNDETDKSIEEIKKAVKTFKRK"
     gene            2701..3165
                     /locus_tag="PKH58_00255"
     CDS             2701..3165
                     /locus_tag="PKH58_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNX87484.1"
                     /translation="MEFSKITNGIVEIIDSWEPKLTNLPIETITLKRNNQNRTIKQIL
                     GHLIDSASNNHQRMIRLQYNTNLTFPDYRQDNDLWIALQDFQSENWNNLIQLWKYFNL
                     HMIQVIKSVDQFKLDNYWHDFEGTKVTLQEMIEGYLWHINLHLNEIQELIDK"
     gene            3336..3671
                     /locus_tag="PKH58_00260"
     CDS             3336..3671
                     /locus_tag="PKH58_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87485.1"
                     /translation="MRYNIGLIASGVLSYILYVALGSVLIAPYDCEFEITILGFVFQG
                     FFFLITILIANIFYNLGAFTDKHYNKKNEEKFRQRLYNLGFWFSFGLPFLVPLLIIVK
                     YFTCYANMK"
     gene            4175..5236
                     /locus_tag="PKH58_00265"
     CDS             4175..5236
                     /locus_tag="PKH58_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX87486.1"
                     /translation="MKDIIPAVDRTLLKSELTPDIYLRPTNKGHNEIYITTAAKSPNV
                     MREIGRLRELAFRSWGGGSGNEMDTDEYDFLKKPYKQLIVWDPEAEEIVGGYRYLSGR
                     DVTMDKDGQPQFVMSHLFRFSDTFIRDYLPYTIELGRAFVQPDYQTAKMGTKSLFALD
                     NLWDGLGALIHKEKKNLYFVGKVTIYDTYPVLARELLYEYMFLHCPDKDELIRPKQEV
                     PVSETGKQKAREIFTGNDAKEDYKALQKAIRHEGTTIPPMFNAYIGLTDTLRMFGSII
                     DPDFSGVYESGIMLTIGDLIDSKRQRYIDPYIAYLKQLLNEKRAAHRAAKIAKGKDDT
                     PSKIKQLAQEIIEKTKRDK"
     gene            5634..6068
                     /locus_tag="PKH58_00270"
     CDS             5634..6068
                     /locus_tag="PKH58_00270"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNX87487.1"
                     /translation="MDEIKFKHTIPIQLRFNDFDALGHVNNSVYFSFYDLGKTEYFRE
                     VIPEMISNQEVGVVIANIQVSFLLPVFPGENVAVQTAVTEIGNKSFKLLQQLIDCETN
                     EVKCICDTVMVGYDAKTKVTRTISDEWRKAINDYEGRGDLSK"
     gene            complement(6138..6452)
                     /locus_tag="PKH58_00275"
     CDS             complement(6138..6452)
                     /locus_tag="PKH58_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNX87488.1"
                     /translation="MENNFTIKAGPYAVLKEFDVEAHHLQEVTDLLKELQINALGEQG
                     CYAFEILQSEENQEKLYLYECFENMTAHAKHTESPYFRELVSGKLNPLLRDKKIIRVK
                     PV"
     gene            complement(6516..8093)
                     /locus_tag="PKH58_00280"
     CDS             complement(6516..8093)
                     /locus_tag="PKH58_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNX87489.1"
                     /translation="MSLQSEIKRRRTFAIISHPDAGKTTLTEKLLLFGGAIHIAGAVK
                     SNKIRKTATSDWMEIEKQRGISVATSVMGFEYRDFKINILDTPGHQDFAEDTFRTLTA
                     VDSVIIVVDAAKGVEAQTRKLMNVCRMRNTPIIVFVNKMDREGRDPFDLMDELEAELG
                     INVRPLSWPIGIGATFKGVYNIYKQNLQLFTPNKQLISESVELADIDSQELDHLVGKK
                     PADKLREDMELINGVYSAFNSDLYMQGHLAPVFFGSALNNFGVKELLDCFVEVAPSPR
                     PVKTLERVVDPYEEAFSGFVFKIHANMDPNHRSCIAFVKICSGRFERNVNYKHIRHGK
                     MMRFSSPTAFMAQKKETVDEAFAGDIVGLPDTGNFKIGDTLTGGEELHFKGIPSFSPE
                     MFMYLENSDPMKAKQLDKGINQLMDEGVAQLFVNQFNNRRIVGTVGQLQFEVIQYRLL
                     HEYNASCRWEPISLFKACWIESDDTVELENFKKRKVQYMAKDKDGRDVFLADSNYVLQ
                     MAQNDFKNIRFHFTSEF"
     gene            complement(8285..9838)
                     /locus_tag="PKH58_00285"
     CDS             complement(8285..9838)
                     /locus_tag="PKH58_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNX87490.1"
                     /translation="MKKNIIVVLAAFVSTLAFAQTEYDAFKLTQTDLSGSARYMGMGG
                     AFGALGGDATALKDNPAGLGVYRGSEIAGTLNTMIQNTGSVWSGMTSKEDMLKVNLNS
                     FSYVLAMPSWSEKNSGLMSSNFSFTFNRLRNFNRKVSAASPGSDNSFTDFLAAFSKRY
                     SEADLQENSTIKDPYENPDNAWLSVLGYQNYLINPQEGNTWSSILDEGETVAPSTYLF
                     ETGALSEYGFGWGGNFNNNLFIGANVNMQDLSYNLHSTIAEDFAKGGYFDLTSAMSQT
                     GVGFNLKLGAIYLPTNNLRLGVSFHTPTFYSISEYTNVLNFKTSEINDSYLPLQGYSH
                     DYKITGPLQAQASAAYLFGKKGLISVEYDYVNYPGMRLGLENGDTQYYKDENQNMPNV
                     YKAGQTLKVGAEYKLSPSFALRAGYALMKSANQTDYAQGKSLKLNSANTNTEYFNQMD
                     TRYLTFGAGYRAAGWFIDFAYAQKMLDEDMYPYQILRYQNNRITNIVTPAAVNTKNNN
                     IVVTLGIKL"
     gene            complement(9902..11329)
                     /locus_tag="PKH58_00290"
     CDS             complement(9902..11329)
                     /locus_tag="PKH58_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87491.1"
                     /translation="MKRFSLLISFLIALVSSGFAQSKKIIDDIYVTPNDAGMVKEVQT
                     AKKTVREKPAYKNGAREIIFIDRNGKRTTAVTDTVYVMDDTVNDSIETVDTVETEGEY
                     LRGFKGSQQDLEYAERIRRFHNPRYTITIADPGYNDIYFLDENDWNVYVDGIYATITP
                     TWTNPYWFNYQYFPYGYSSWGWRNSLYSSFGWGYYDPWYSPWSSWYGGYGYGWGGYYD
                     WGYPYYGGYYGWGYPYYGGYYGYGGLGYPYSGNGYYSGNNHNTRENGRVGRFESTRPT
                     SRTSVTREQAVLGSGRTSGSNLYTTVSRQGTQRNRFESNNLSTSGRGMQNTTSGRGVQ
                     TRSSYTSSERNSYSPNVNTTTRTGAVRSSDQWNATRNSNATRYSNETRSSSGSSTVIN
                     RSGSDSRSSGSRSYNSGSSSSRSSSSYSPSYNSRSSNSSTYSSGSTSSGSYSSGSSGS
                     YSSGSSSSSSSSSSGSSRSGSGGRR"
     gene            11541..12383
                     /gene="prmA"
                     /locus_tag="PKH58_00295"
     CDS             11541..12383
                     /gene="prmA"
                     /locus_tag="PKH58_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNX87492.1"
                     /translation="MKYTAIHFTFDTSVEYTADILASMLGDTGFESFVNTDKGLDAYI
                     PSKLYDENALKVTLKNFPFPEEIDFEKEEMEDKNWNEEWEKYFFEPIIINDECIIHSS
                     FHKDIPKLKYDIIIDPKMAFGTGHHETTSLMIGEILNMDLAGKSVLDMGCGTSVLAIL
                     ASMGGAGDLTAIDIDDWCVDNSLENIQLNNVSGIDVLLGGAELLEGLHFDIVLANINR
                     NILLNDMQSYANTLPAGGELYMSGFYTEDVPVIETKARELGFEMLYSKQKNNWAVVRM
                     LKKF"
     gene            complement(12468..16106)
                     /locus_tag="PKH58_00300"
     CDS             complement(12468..16106)
                     /locus_tag="PKH58_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX87493.1"
                     /translation="MNRNNAEKQRILNRNSVALILLCVFALLGVVCQWFYKQSPANAI
                     PINDVRYAISVKQRKAEEKMKVIEGLLQRKSLDSLNLISFQKLANTYLVYRNGELIFW
                     SNNITSQKNLQNTAWEYVLMPNMHTIAKSKKISEFNIVAYIPVKYNYSYENKELTNKY
                     FEGFKLNKEIGIVLNKPADKYAIFSNQNDYLFTLKLPDQPVYNENWAIAAMYFFLAAF
                     FLLFYLFATFPLLIGKKLFTWKEFLEVTSIMTLFVIICLKFNLPTTFFSNKIFTPYHY
                     ATNSILASLTHLSFLTSYICSTIYLFCFHTDRNLRKDKYFTLKAVVLLLIPGAYFMLI
                     FNFMVGLVFNSTTELNILKLTDFNWVSVWNHFLLFFLGISYMSLYLKIHRIILRKKGL
                     RGLLLIDIPVTLFFCVLLFFNLRPYGLDAAVSFLIPNIILYLPLLYRGLMDNKWFVAI
                     WLFFITLFVTWNFINMNKDKKFEKYKTLAENHYLNDNADEDKIAELILVDIDREVKKD
                     EVVRHLMQYPDSVQKANDYLNKAYLRGFWSKYEMRLFATLPGTVLDQTYQETINSYGN
                     QIKSSNFFVMNSKSSDMAFLGAFKLEQSGGKKVNFYMEFYPKKYYKSYSFPGLLVEGT
                     PSLQQQLELSSARYTYHELAFTSGKFRYPRDGKWIDKKPLNFYTQDYGSYRHYIYVPN
                     IYNYFILSEENTNSIWGYLLYVLYTFVVYLLVAFLIFWIYKLIHKKTSNPFRLTSRYL
                     YSFVVLLIISFVSIFYVSINYIQQKYRNEQRINLERTKNYIQTALQEKYYWTEKIDSS
                     MENSLNLFLQDLSYTYQNDIHVYDNEGKLIASSQPSIFSRGLISKRISPLPFFSKNPN
                     MNNYEFIGELEYLTAYTDFYNGDFLQIGYIAVPQYFSNDQIQAEIQEFLAVIVHIYLV
                     ITILLIFLSLVINKKLSEPLAMLERSLKDIRLGQRNKKIEYKPNDEIGQLVIQYNKTV
                     DELEESARLLAKSERESAWKTMARQVAHEINNPLTPMKLTIQQLQRTKKMGDNRFDEY
                     FEKSTVTLIEQIENLSQIAGTFSTFARLPEAKPDKVDVSKIVGMVVELFSNNHDGTRI
                     IFRSDVRDAFVIADREQLIQVFNNLLKNAIQSIPTGREGKIQVSVETTPENVFVHIRD
                     NGKGISDDIKDKLFTPNFTTKSTGMGLGLSISQNMIRMAGGQITFETKIDEGTVFHVI
                     LPKYMTEL"
     gene            complement(16099..16572)
                     /gene="rlmH"
                     /locus_tag="PKH58_00305"
     CDS             complement(16099..16572)
                     /gene="rlmH"
                     /locus_tag="PKH58_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNX87494.1"
                     /translation="MKIKLLMVGKTTDPRLTELIDGYAGRLKHYIGFELVVVPELKNA
                     KSLSVELQKEKEGEQILGKLENPDHVVLLDEKGKQFTSVRFSELISNLMLTSNKCTVF
                     VIGGPYGFSEQVYKRANSMISLSSMTFSHQMIRLLFVEQLYRAMTIIKGEPYHHE"
     gene            16752..17156
                     /locus_tag="PKH58_00310"
     CDS             16752..17156
                     /locus_tag="PKH58_00310"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNX87495.1"
                     /translation="MKTYKFIALIAFFSLITSQNITAQNVDVPNEITVALNSGDAGKL
                     SAWLNNNVELVIGDKNDVYSKQQAIGIISDFFKNNSVLGFQVLHKGNREAASFVIGTL
                     KTSSRNYRVYVLTRRTGKQSLIQQLRIETNNE"
     gene            17149..17991
                     /gene="nadC"
                     /locus_tag="PKH58_00315"
     CDS             17149..17991
                     /gene="nadC"
                     /locus_tag="PKH58_00315"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNX87496.1"
                     /translation="MNKDFIKMIPAWFAEDIGDGDHTTLSCIPPDAMGKSQLIIKEDG
                     IIAGVEVAREIFKAFDPELKMTVFIQDGSEVKYGDIAFVVEGKIQSLLQTERLMLNIM
                     QRLSGVATRTHEYMQLLKGTRTSVLDTRKTTPGLRMLEKEAVKLGGGTNHRIGLYDMI
                     LLKDNHVDFAGGIEQAITRTKEYLKEKGKNLKIEIEVRNFDELQQVLNIGGIDRIMLD
                     NFSVENTRKAVEMIGGKYETESSGGITKETLRDYAECGVDYISVGALTHSIKSLDMSF
                     KAVE"
     gene            18143..18772
                     /locus_tag="PKH58_00320"
     CDS             18143..18772
                     /locus_tag="PKH58_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNX87497.1"
                     /translation="MDQNKPLVITISRQLGSGGAYIGHQLAKRLSMYYADHEIITKTA
                     QELRMLEKDLEQQEEKFESFWKTFLRYNVSAPEVYIPSSYNFFPTTQQLFETESDVIK
                     HIADESSSVIIGRCGFHILRDHPNKVSVLIYCDLEKRVQRIKDIRHMTEEEARKTVLQ
                     NDKERELYINNFSKKKWMDARNFDICIDTGKISFDNSVELIINYLNLRK"
     gene            18968..20320
                     /locus_tag="PKH58_00325"
     CDS             18968..20320
                     /locus_tag="PKH58_00325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNX87498.1"
                     /translation="MKIAIIGTGYVGLVSGTCFAEMGVDVTCVDVDSTKIDNLHKGII
                     PIYEPGLEDMVNRNVKAERLHFSTRLDDVLDDVEVIFSAVGTPPNEDGSADLKYVLQV
                     AREVGQKMNKYVLLVTKSTVPVGTARLVKQAIQEELDKRGVSIPFDVASNPEFLKEGD
                     AINDFMSPDRVVVGTESEKAKELITRLYRPFLLNNFRVIFMDIPSAEMTKYAANSMLA
                     TRISFMNDIANLCEIIGADINMVRKGIGTDVRIGSKFLYAGSGYGGSCFPKDVQALIR
                     TAEENGYELKLLKAVEEVNNTQKEVLFRKLAKRWGHDMSGKTVAVWGLAFKPNTDDIR
                     QAPAFDIIRKLLLAGATVRCFDPIAIPASKSYLEYLEKDNNTTEKPDYSKVYFANDIY
                     DATFDADALLLVTEWKEFRMPSWNVVKKSLKNPLVLDGRNIYDKTEMKELGFEYECIG
                     "
     gene            complement(20448..21368)
                     /locus_tag="PKH58_00330"
     CDS             complement(20448..21368)
                     /locus_tag="PKH58_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX87499.1"
                     /translation="MKVLIATDKPFAKVAVDGIRNEIEGVGYELALLEKYTEKQQLLD
                     AIADADAVIIRSDIVDEEVINAGKKLKIVVRAGAGYDNVDLEAATRAGICVMNTPGQN
                     SNAVAELVFGLLVFAVRNFYNGTSGTELMGKKLGIHAYGNVGRNVARIAKGFGMEVSA
                     YDAFCRPEDIKAEGVNPTCSVKTLYQSCEMISVHIPLTPETKQVINKELLELMPKDAV
                     LVNTARKEVVHEADLLQHLVDNPKFKYVTDVTPDTDEEFKVKVGDRYFATAKKMGAQT
                     AEANVNAGIAAAQQIVDFLKNGNQKFRVNK"
     gene            complement(21556..22635)
                     /gene="serC"
                     /locus_tag="PKH58_00335"
     CDS             complement(21556..22635)
                     /gene="serC"
                     /locus_tag="PKH58_00335"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNX87500.1"
                     /translation="MKKYNFNAGPCVLPKSAIESAIEAIRDFDNTGIGILEISHRTPG
                     WERIMAETEQLWRELLNIPEEYAVLFLGGGASTQFFYVPANLLGKKAAYLQTGVWAKK
                     AIKEAKFYGEVEVVASSEDKNYTYIPKSYTIPTDADYFHITTNNTIYGTEIHEDINSP
                     IPLVADMSSDIMSRPVDVSKYALIYGGAQKNVGPAGVAFVIVRKDLLGKMDRKLQTMV
                     DYRTHIGETAKDNSMFNTPPVFSIFVMHETLKWVKDLGGVEAMYKINLQKANLLYDEI
                     DRNKMFVGTAVKEDRSIMNVCYVMAEEYKDKEADFMNFAKERGMVGIKGHRSVGGFRA
                     SIYNACPLEAVEALVACMKEFEKMN"
     gene            complement(22888..24291)
                     /locus_tag="PKH58_00340"
     CDS             complement(22888..24291)
                     /locus_tag="PKH58_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNX87501.1"
                     /translation="MKKQIVFIFALFVSAVLFSQSPEGSWNGLLKVQGQQIRLVINIQ
                     QAENGYKATMDSPDQGAKGIPVDLVTFTNDTLKFEVKMIGVTYTGVLGEDKVIKGTFA
                     QMGMSLPLDLTTTKIEKEKVLRPQEPKAPFPYLSEDVKFINSIDGDTLAGTLTMPKKE
                     GKYPVVVLISGSGAQNRDEELMGHKPFLVIADFLTRNGIAVLRYDDRGTAQSTGDFQK
                     ANSTGLANDAEAAVSYLLTRKEINKKKIGLAGHSEGGIIAPMVAARNRKVAFIVMLAG
                     PGLRGKELLELQTAAIAKASGMTETELANILRLNSGAYDLVLHSNDTAKLKTDLTDYL
                     LTTLKDDPTLGENPTLEQQDRQAKGYTDQLASPWMQYFIKYDPFPTLKKVKCPTLALN
                     GSKDLQVPPAENLTLIRKAFGESGNKRLTAIELPGLNHLFQECTTGSPSEYAVIEQTF
                     SPLALNEMLKFIKQQTK"
     gene            complement(24309..24881)
                     /locus_tag="PKH58_00345"
     CDS             complement(24309..24881)
                     /locus_tag="PKH58_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87502.1"
                     /translation="MKNKINLRYIYNPFEKIAGWQALLYGLVFVVLSGWIGKHAGLVF
                     DGAIDAHLSNDVTYYQALIILVIDVVCMSIVMYVAGLFVTKNFRFIDLLGTMTLARAP
                     YIVLSLAGLFVTVPETADLLANPYRVFTNPGFLVFIVLTIPVMVWVIALMYNAWKVST
                     GAKGPKMIVGFIAGLFVAEVISKILISIII"
     gene            complement(24871..25134)
                     /locus_tag="PKH58_00350"
     CDS             complement(24871..25134)
                     /locus_tag="PKH58_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HNX87503.1"
                     /translation="MLPTSCPSCHAHLKVRSLKCEQCETEVNGLYDLPVLARLSDEDQ
                     RFILSFVKNSGSLKDMATELKFSYPTVRNMLNEIILTIEKYEK"
     gene            complement(25346..27961)
                     /gene="mutS"
                     /locus_tag="PKH58_00355"
     CDS             complement(25346..27961)
                     /gene="mutS"
                     /locus_tag="PKH58_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNX87504.1"
                     /translation="MENEVVETPMMKQYFEIKRKHPDAVLLFRCGDFYETYSTDAVEA
                     AGILGITLTRRANGSAKTVEMAGFPHHALDTYLPKLVRAGKRVAICDQLEDPKLTKKI
                     VKRGVTELVTPGVSYSDTTLTQKENTFLASVYMNKNQAGVSFLDISTGEFLLAEGTFD
                     YVDKLLNSFSPKEVIYDRTKRNEFETNFGSKYFTFALEDWGFLPETTNERLLKHFQTK
                     NLKGFGVENLKNGIVAAGAILHYLDMTHHHQTEHITHLTRIDEERYVWLDRFTVRNLE
                     LYGTINEGGRTLVDVIDRTISPMGGRLLKRWVAFPLKDVKPIEERLSVVEYFFRDTEL
                     KHMLEQQIALIGDLERIISKVAVGRINPREVVQLKVALQAIEPIKNACLAIENVTLRK
                     IADQLNPCKVISEKIEKELQNDPPVLVNKGDILRPGVNAELDELRHLARSGKNYLLEI
                     QQRESEVTGIPSLKISYNNVFGYFIEVRNAHKEKVPDTWIRKQTLVNAERYITQELKD
                     YEEKILGAEEKILAIETRLFNELIISLSDYIAAIQLNSNLIAQLDCLLSFARVSAENK
                     YVRPEISDSDVIEIRQGRHPVIEKQLPVGENYVSNDVFLDGKTQQIIIVTGPNMSGKS
                     ALLRQTALITLMAQIGCFVPVESAKIGVVDKIFTRVGASDNISQGESTFMVEMNEAAS
                     ILNNLSDRSLVLFDELGRGTSTYDGISIAWAIVEYIHEHASCKAKTLFATHYHELNEM
                     EKTFNRIKNYNVSVKEVDKKVIFLRKLVRGGSEHSFGIHVAKMAGMPQSIVKRADQIL
                     KQLETDNRQNGDLSKPVGEIGNQREGYQLSFFQLDDPVLEQIRDEIKGLDVNNLTPIE
                     ALNKLNEIKRIITGK"
     gene            complement(27969..29087)
                     /locus_tag="PKH58_00360"
     CDS             complement(27969..29087)
                     /locus_tag="PKH58_00360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX87505.1"
                     /translation="MKIIIVGTAYPYRGGLSAFNERLAREFVRQGNEVEIYTFTLQYP
                     SFLFPGKTQFSDDEAPADLKITRRINSVNPLNWISVGKEIKGKNPDKIIFCYWMAFMA
                     PCFGTIARFAKTEKTKIISLVHNMIPHEPSVLDKLFPKYFVKAMDGFVAMTNSVVNDI
                     SKFDTKKKPKLVSPHPVYDHYGAILPRSEAMDALNLDPDFRYVLFFGFIRRYKGLDLL
                     IQAFADVRLRQFPVKLLVAGEFYEPKEPCFEMIKKYALDDLVVLHTDFIPEKEVAFYF
                     NAADLIAQPYRSATQSGVSQIAYHFEKPMLVTNVGGLADIVPDGKVGYVVEPEVNKIA
                     DALVDFFENNRSDEFIKNVKEEKKKYDWKMMVQTINKI"
     gene            complement(29464..30150)
                     /locus_tag="PKH58_00365"
     CDS             complement(29464..30150)
                     /locus_tag="PKH58_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX87506.1"
                     /translation="MNPYRILVVDDEEDLCEILQFNLETEGYQVDVAYSAEEALKKDL
                     TVYHLLLLDVMMGEISGFRMARMLNENPKTSSIPVIFLTAKDTENDRLTGFNIGADDY
                     ISKPFSIREVIARVKVVLRRVDKNSEPEMPSTLTYETLTMQLDNKKVLLDGQEIPFTK
                     KEYEILKLLLENMNRVFTREEMLSRIWSDEVVVLDRTVDVNITRLRKKIGPYGKQIVT
                     RLGYGYCFEG"
     gene            complement(30376..32877)
                     /locus_tag="PKH58_00370"
     CDS             complement(30376..32877)
                     /locus_tag="PKH58_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HNX87507.1"
                     /translation="MKRKNLLAAVLIAAISAGVWAQNRTEYLLEKNWKFIRTDQPDQM
                     NAAYDDSKWQSVTVPHDWAINGPFSINNDQQKIAITQDGQKEAMEHAGRTGGLPFVGV
                     GWYRNTGNLPAYQAGKRVTVLFDGAMANSQVFINGRKVGEWPYGYNSFHFDVTDFLNR
                     DKTPNVLAVRLENRPEQSRWYPGAGLYRNVHVIITDDVHIPVWGTYVTTPVVRQDFAK
                     VNIRTRVEGNNTPNVNYRLSTDIVNPFGVIVAKNEKQLTKFDENEFSQDLIVENPRLW
                     STTEPTLYKAMSKLYANDRLVDEYSTTFGIRTIEIKPNDGFYLNGTKTVFQGVCNHHD
                     LGPLGTAVNDAAIRRQVRIMKDMGVNAIRTSHNMPAPELVRACDEMGMMIMAESFDEW
                     KIPKVGNGYNLFWDKWAEKDVVNLVHHYRNNPSVVMWCVGNEVPEQGDAANGPKRNRF
                     LRDICHREDPTRPVTNGMDNSNAVINNNFAAVLDVPGFNYRPHLYTENYKKLPQQIIL
                     GSETASTVSSRGVYKFPVERKAMAQYDDHQSSSYDVEHCSWSNLPEDDFIQHEDLPYA
                     IGEFVWTGFDYLGEPTPYYTNWPSHSSLFGIVDLAGIPKDRYYLYRSHWNKNEETLHI
                     LPHWNWKGREGQVTPVFVYTNYPSAELFINGVSQGKRTKDLSIKRDGSDTDEARKNFD
                     RQKRYRLMWMDTKYQPGTVKVVAYDENGKAVAEKVMKTAGQPYRIVLEADRTSITADG
                     KDISFVNVKIVDKEGNFCPTEEREISFNVKGAGIFKAVANGDPASLESFQAPKMKLFS
                     GQLTALVQSLEKGGSITLEATAKGVKDGKITIEAK"
     gene            complement(33018..33464)
                     /locus_tag="PKH58_00375"
     CDS             complement(33018..33464)
                     /locus_tag="PKH58_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNX87508.1"
                     /translation="MAEFAEWGYLGLFIASFLAATVVPFSSEVVFSALVFGGLDAWQC
                     VLVATLGNWLGGMSCYYLGMLGKVEWIEKYLRIKKEKMDSFEAKLHKYGDWFAFFSFL
                     PGIGDGIAVASGFFRCRWWIVAVSMLLGKFVRYVVWMYANGFIMPR"
     gene            complement(33481..34464)
                     /locus_tag="PKH58_00380"
     CDS             complement(33481..34464)
                     /locus_tag="PKH58_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87509.1"
                     /translation="MKLSLQKIIAPSFAVTALTYTVSFGLWMGMCFLYRYPNPMPGIA
                     GVVAENILSSGSVLAYLITFAVTVLNSLLIAQMNNKFAFIRTRTFMPVFIYLLLSTCW
                     LQIHGNYLAVIASFFLLVAVFLSLQMYKDTWGVEQAFLAFFCVSLGSLLVHDLVYLLP
                     FFWIGFILLRCFSFRTFFASVFGFITPLIFVFSIMFFVMNQNTLIPDMKGVVFDFQFL
                     NYDNIPQLIYFGVIFLIFSLAMTGLSGNTRHDVIQARNMLFFLRVMAFGLLMLLIFRF
                     SGYTYYLPLIAALFAILTAYTFTLVKNLFYYIVFIVLCVVSLAYVVYQLYF"
     gene            complement(34587..36683)
                     /locus_tag="PKH58_00385"
     CDS             complement(34587..36683)
                     /locus_tag="PKH58_00385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNX87510.1"
                     /translation="MKKLFFAMMIGTVVMACTSKTDNPFFKSYENEYGAPPFDKIKTE
                     HYMPAFSEGIKQHQAEIDSIANNTEEPTFANTIEKMDFSGVLLKKVSSAFFNVQSADT
                     NDDLDSIAQEITPVLTEHNDNIYLNDKLFQRVKKLYDNRANLGLTPEQNRLLEKYYKN
                     FIRSGAALNDAQKSQLREINKELSSAELTFAQNVLAETNAYKKFVSNKDELKGLPEGV
                     IQAAAESAKEAGKEGQWLFTTQKSSFIPVLQYAENRELRKELLMAYTTRCNHDNANDN
                     KKVINTIMKLRVKRAQLLGFESPAAFILDNTMAKTPEAVYTFLETIWTPAVAKAKVEA
                     ADLQKLMDAENKGEKLEAWDWWYYTEKLRKQKFDLNEEELKPYFKLENVREGVFGLAT
                     RLYGLKFEKLTGMPVYNKDVETFKVLDADGSLIGILYTDYFPRAGKAAGAWMNNITEQ
                     YIKDGVNHRPIIVNVGNFTKPTSNKPSLLTMDEVETMFHEFGHALHGLLAQSTYPGLS
                     GTSVVRDFVELPSQIMENWCWEPEVMKTYAKHYKTGELMPEALMEKIRKAGTFNQGFI
                     NTELLAASLLDMDYHTLKDTAAIDVNQFEANTMKRIGMIPEIIVRYRSTYFKHIFEGG
                     YSAGYYSYTWAAVLDADAYQAFKETGDIFNKDVATKFRKNILEKGDSDEPMKLYKTFR
                     GADPNPDALLIKRGLK"
     gene            complement(36788..37714)
                     /locus_tag="PKH58_00390"
     CDS             complement(36788..37714)
                     /locus_tag="PKH58_00390"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNX87511.1"
                     /translation="MYIFRYNDTIPWDNCVATVGFFDGVHAGHRYLLQSVRDIARDEH
                     KISVVITFDNHPRKILHADFQPKLLTTLDEKISVLSETGVDACVVLNFTKDLSQLSAF
                     SFLKDVLHNTLKVSTLLVGHDHRFGHNREQGFDEYRAFGATLGMNVIQVEKYTTDEAH
                     HISSSEVRHALERGEIAQANKLLTYPFILTGRVKSGFQVGRKIGFPTANLTPSDKDKI
                     IPATGVYAVRVKWDNHTFRGMMNIGYRPTMGHSDEKSLEVHILDFEGDVYDRNLKIEF
                     IAKIRDEMKFSSVDELIIQLEKDKDTVIAMDY"
     gene            complement(37741..38358)
                     /locus_tag="PKH58_00395"
     CDS             complement(37741..38358)
                     /locus_tag="PKH58_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNX87512.1"
                     /translation="MSKYKNVTTIIFDLGGVIIDLDWNLCIHNFENLGIFNMKNLVST
                     TLQKGFVLDYEMGMITDAQFRDEIRKNASGEVADHQIDHAWTSLLVGIPEEKLNLLSE
                     LKKKYKILMLSNTNNLSFRYSEKMFEVNGKNINDFFDKCYLSYKMKLAKPKPEIFEAL
                     LEDAGVKAEECLFLDDGIHNIETASELGMQTWLVEPYTSLSLNDF"
     gene            38774..41350
                     /locus_tag="PKH58_00400"
     CDS             38774..41350
                     /locus_tag="PKH58_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87513.1"
                     /translation="MIKKINSRLCFLLVIIGFICGSIQAQGLKFYGIKASIEERTSFF
                     VFEEGSSPVFSQYIDLDFELKIQEFGSFGYLLHIVDNQNNTAYSFTYTNIDKLSSAFK
                     FNIEGKANLISMNVVNESIKSKWLPVRLHIDLTTGQSSLSINNITNNSEKKLNISEKF
                     QPLLVFGRRENLVDVTPFAIRNLKISGANNSFRFLLNESTGEAVHDTNGKVRGRVIHP
                     YWLINESFHWKKTVTIPSKSIMGSKFNEKEQCIFFINQDSLFRFRSGSNLLSGEPYAN
                     KLPVKLLLGTNYIDESSNKICAYEINNLPAGDITMAVLDIPTRTWKAAGRAFTPVQLH
                     HHNGLLNKDGQYLVFGGFGNKRYSNNFLAYNAATDRWDTLTFKGDRITPRFFSSIASS
                     KKGDVLYIYGGVGNDSGDQTVGHNYYNDLYRVNLVNHTIKKCWGNPSNTKLVSSERMI
                     LSDDEKYLYVIRYPEYNEITHLQLHRISVADGTSQLLGDSIPYISKSIASNVSLYYNA
                     ALQEFYCVTQEFDDFAHSVNAHVFTLSAPPVDKTAIQFYSAPKSNSLFIIMIILVLVL
                     SAGFILFFYLRKKQRKNKIELSTVQVPVQIKSTIASAHTKTPDITDLPIKETGSTTEF
                     NKIYIYGIFTVYGRTGRDISYMFSNKLKLIFLYILLNSDSEGVSSSLLNSLFWPEKME
                     KKAKNLKGVTISNLRKALAEIDGVELIYDKGFFRIITTAPCYCDYCHLKVLLDLNSHD
                     SEEMLRILERGQLLECTKQEFFDKYKQQTEDLIFSVLPQELPVLYKKNEHKQVLRICS
                     VMLKLDPLYEPALAYSVYSHCRLNEYEKLFKTYALFAAEYRNSMGQTYPKSLETLIQE
                     AQ"
     gene            complement(41455..43683)
                     /locus_tag="PKH58_00405"
     CDS             complement(41455..43683)
                     /locus_tag="PKH58_00405"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HNX87514.1"
                     /translation="MKSPKFVLILTLYLCIANQAKTQVLYENDAYRWQSDKIIQGNYV
                     GTAESTTKIHSNFSSPSSKDDLISYVWTKKNDISSYPQYSGPTLLENAIYNLGLDEMV
                     NAVEPDLTLRTGILWPGVWTRDVSYSIFLSMSYMQPTVSKNSLLRKVDMVGRIIQDTG
                     TGGSWPVSTDRVVWITAAWELYKVTGDFDWMKSVYPAIKKSVETDLQTVYDKETGLAM
                     GESSFIDWREQSYPKWMQPADIFQSKCLGTNAIHYQALNIVSKMAKTLNKQDDYMRYS
                     QKAESLKAAINKHLWMPDRGYYAQYMYGRNCNVLSPRSETLGEALCILFDIASPEQQK
                     QIIQNMPVLPFGPSIFYPQISDMFSYHNNATWPFVTSYWMLAAAKVGNQKAVEHALGS
                     TYRATALFATNKENFDVQSGDWTGTHTNSSNMLWSLSGNLSIVYRLYFGMKFEEDKLI
                     FNPFVPKELAGKRNLTNFSYRKAKLNISMEGFGDRIALFTIDGKSGTPEVLSTMSGEH
                     DVKIILTNSFVDSDSVINLTDNKHTLLNPVAKINDGILSWSPINGALSYTILKDGREY
                     KKLKNTSLKLKSTDEGEYQIIATCNDPILSSFASEPLVWRKQSETIYNIDETRIEKSA
                     NITKSGEKVSTIFSTTIKVAKDGFYAIDWLYANGGATVQDENKCCIRSLSVDGMYEGV
                     SIFPQRGRNEWQNLGWSNSTHVNLKAGEHQVQLQYRPENENMNREVNDAIIHKLRVTL
                     LK"
     gene            complement(43869..46517)
                     /locus_tag="PKH58_00410"
     CDS             complement(43869..46517)
                     /locus_tag="PKH58_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNX87515.1"
                     /translation="MKTKSILTLLVLLAFDTILYAQESEENPIKKLFIDQLSAYPQEK
                     IYVQTDKGGYYPGETIWMRVHLVDAIFMKQANASRYVYVELINPAKHLVQRVKLRPDS
                     TGCFYGQIQLDEEIGEGDYLLRAYTRFMRNAGEDYFFSKQLYISNPVSEKVSVDINYK
                     AEGDGVNAELLFSAKGRKEKLMPGQCVVYLGGTEKGDAKTVTFKEKTGSCSFKGKEVS
                     KERVFLLQAVIENRMYKRYFKIPDIENTFDVSFFPEGGYAPSSANTTIAFKALNTQGQ
                     SEEVKGQVFDDQHYLCAEFESTHLGMGSFKFSYEPGRKYYALCTNKEKVVKRFDLPEP
                     SDSAVSLKTVWEDDKLLVRVLKSPDFILPPQMQLIVHIRGVPIYVEPWDENKSCIGFE
                     KDFFPAGIVHFLLVDKDRNILSERLVFSSQGNALVQTNIELNKEKYQTREKINLSVHL
                     TDENKAPLSGNFSLAVVDANGADIDSASNIISALLLTSEIKGYIEDPASYLRQDSKKT
                     EAALDVLMMTQGWRRYDIPNLLKGKLTKPLQYPVELSEEISGKAESVFSSLKEGDISL
                     LALKDSVEGMLLAKTDNEGRFVFKNLEYCDGTRYIIQATSNSKFKKAFLEMDSIAGFP
                     SLAASQIINRKESILKDTILLPSNKKHLAFDGMKTYHLSEVMVTARRKVAAVTESPYY
                     SVTSSRVITAKDIEGWHVSSTYELLRRLAGVTVNGSDVRYRGNKPMLLLDNVPTEDFD
                     YSMLMIDDIQDIFINQGSTMGAIFGTMGSNGAIVINTKNGFVQKNSINPNIKVVKSLG
                     YQQTVKFYSPVYSTEQEKNSSTPDYRTTILWNPNVQVDSTGTVNLSFYAADIPTTYRI
                     ILEGVSSLGHLIYSAEKEMLVVSDKQ"
     gene            complement(46714..48969)
                     /locus_tag="PKH58_00415"
     CDS             complement(46714..48969)
                     /locus_tag="PKH58_00415"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX87516.1"
                     /translation="MRLKKNLLLLGIMAYCSCMGSNVKLPVDYVNPLIGNISHLLVPT
                     YPTVHLPNSMLRVYPERADYTSDVIHGLPVAVTSHRGSSAFNISPIQGKAVSNSRVQA
                     YSYDLEKITPYSYSVFLDEANIHVNYSPSYHSGIYEFNFDKEDDNYIVVNVGNGNLET
                     DGKSISGFQVIDNSISKIYLYVETEQIPTSVGRWGKDQINYQVKQSGNNGALVLRFNK
                     NDKNIRLRYGISFISTEQAKKNLNKEITVYDVEKVALAGRAVWNATLSKISIEGGTED
                     QKTVFYTSLYRTYERMINISEEGKYFSAFDGKIHDDGGHAFFTDDWIWDTYRATHPLR
                     VLIEPEMEANMVNSYIQMAQQSPQGWMPTFPEVTGDSHRMNGNHAVAVILDAYSKGLR
                     GFDLKAAYKACKGALTEKSLLPWMKVPVTELDQFYNSNGYFPALKPGEKENCAQVHSW
                     EKRQPVAVTLGTCYDDWCLSQIAKQLNKTDDYQYFLKRSYNYRNLFNPETSFFHPKDA
                     EGKFIEPFDYRFSGGPGAREYYDENNGWTFRWDVQHNVADLVKLMGGADKFARNLDAT
                     FREPLGKSKYDFYSSLPDHTGNVGQFSMANEPSFHIPYLYNYAGQPWKTQKRIRQLLR
                     YWYRNDLMGVPGDEDGGGMTAFVVFSSMGFYPVTPGSMSYNIGSPLFNDVKVRLGNGK
                     IFEIVAKNCSEENKYIQSALLNGKEWNKPWFNHSDIANGGSLILIMGNKANKNWGSNL
                     EDAPPSATPIP"
     gene            complement(49091..49342)
                     /locus_tag="PKH58_00420"
     CDS             complement(49091..49342)
                     /locus_tag="PKH58_00420"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HNX87517.1"
                     /translation="MDEDFAGIYEKYYTESEILDLVTFYKSSTGKKVIEKAPEIQGEI
                     VTIVMQKFMPEIRKNVTEMAMKVKDELEKRNAALKKEKN"
     gene            complement(49373..50395)
                     /locus_tag="PKH58_00425"
     CDS             complement(49373..50395)
                     /locus_tag="PKH58_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNX87518.1"
                     /translation="MKKLSILFILLSFSVFTQWSIAAKPKSLKFNPDGTFKIVQFTDT
                     HICYSRLDNSMKTIETMRSVLDFEKPDLVIFTGDNVVDLPLKEGLQLLVEPVVSRHIP
                     YIILFGNHDTEPGMKISREMLGKMTRELPGYYNIRPVKQIRGENNYLIEIKNAGTTKT
                     EALLYCMDSNDYDSIAGKRVYAGFSPGQVEWYRKMSKKQTNNNHRQPVPSLAFFHIPL
                     LEYADLSDKSTNYIGMYKEKPCPPVINTGMFAAMVKGGDVMGCFAGHDHDNDFVFNKF
                     GIYLGYGRFSGSNNTYTHYENGARVIVLQQGQRKFSSWVRLKDGTLLHEFAYPDAIPM
                     LNAPTF"
     gene            complement(50600..51274)
                     /locus_tag="PKH58_00430"
     CDS             complement(50600..51274)
                     /locus_tag="PKH58_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNX87519.1"
                     /translation="MKLLKTIFFLSVLLGSTSVQAQGNHDWANFGRYAEANKTVKQPI
                     RAVFMGNSITEGWVGADPDFFTDNNYIGRGISGQVTSQMLVRFQADVIQLKPKAVVIL
                     AGVNDIALNNGYISVQHIFENIASMAELAKQHKIRVVLCSVLPAHHFPWRGEVESINQ
                     IRELNEKIKSYALENKIAFADYYSAMVDDRQGLNVKYQRDEVHPNLEGYKMMEQVVRK
                     VLSKIL"
     gene            complement(51310..52725)
                     /locus_tag="PKH58_00435"
     CDS             complement(51310..52725)
                     /locus_tag="PKH58_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87520.1"
                     /translation="MKNIILVFALMFTVFLQAQTLRSDNERLNDAYRLAVNTLDINTR
                     RGILAAGGDYGGEWTRDIAINSWFGASLLRPQVAQRSLWSVTINKESIGHQYWDKIIW
                     VIAAYNHYLVTGDKDFLRQAYLCSVTTIKELETTVFDPKYGLFMGPSVFNDGIAAYDE
                     PIFNPQVHSSATVDHLKPFTVKCLSTNCVYYGVYKTLIEMQKQLRMEASLSTPIVEKA
                     EALKSNILKYLYNPERGTFNYLIDQNGGVDHSQEGLGLSFAVVFGVVDKEQALRAIAN
                     TVVSDYGITSIYPDYKRFSPQMPGRHNNIIWPMVNGFFSNAAVFVGSYKTYDHELNSL
                     ISLALDEDKGNYSFFEIYNPYTGKPDGGYQFGENNPEHHWVSCRLQTWSATAFINMVH
                     SGLLGLRLMPDGILFQPHLPDGIQVAELSGIRYRNISLDITIKGKGSRIKSFRINGKQ
                     QANPFVSSSARGNVAVVVELM"
BASE COUNT        15770 a        11076 c        10158 g        15837 t
ORIGIN      
        1 aaaacgccta acgctaaatg agaaaataca cttgcggatt ttaggtacaa tcgtgtggca
       61 gcaaaggtaa atcaacagaa taaaaatttc atataaacac ctaaagcgga aataggcgct
      121 acgtcatagc gtatattgaa aaaaagtgta gcgcatattt aggtattata ccgcattcaa
      181 aaaaaagata gtgcaaatat taatctttaa acaataaaga aatgccatta ggagaaagag
      241 aaacacaccc atacttagaa caaggacaaa ttattggggc aacaaaattg attttaggta
      301 gttttcctgt ttatgattgt acggacaacg acaatgatct taaaaaacaa aatcgacaga
      361 ataaaggaac agttagattt ttttatggta gttatagaaa tagtctctgg ataaaatata
      421 gccagttcat tgacaacact atttcaaaac cttggaacac agaactaata ctagaaagtt
      481 taagaaaaag aaaaattgct gtgagtgata cgattctttc ttgcgaaaga tataaataca
      541 aaattgacaa aaaaacaggt acgaagattc tttttcccta cagttcagaa gactctgctc
      601 taaagaataa aaattggaat agagttatta tcagaacttt aataaataat ggagtgacta
      661 aaatattatg tacttcaaaa ggtgttttaa atgacttaga aagacaaata atttgttttg
      721 gtagaaataa ttatggaaga aaagacaacc aattaagctc tttatttcaa tctcaattta
      781 ttgaaagggt tggtggaaat aataaccaga ttacaaatga tattgcaaaa atatttatcg
      841 taggaaatag tcaaataatt gcgattgcat taccctcgcc tggtgcacct caaagacaaa
      901 cacacgaatt tggttgcaat aatcaaaaca ggctagaata ttctgaaaat tattttcaaa
      961 atgcatttaa ctggttactg aatgattgag aaaccaaagg actaaccact aaagcatgcc
     1021 ccgatagttg accgaaagca aaaacagaag aaaacataca ataactacat gaaataaaat
     1081 taatttctaa atacaaccaa tattatgaaa acaataatta caatcgcatt cttgttattt
     1141 acagtaaatt cgtttggaca aacaagaact tatagacttg aggatttaac tgagttttca
     1201 aaaaaccatt atggtttttt gaaaactgat aagacaatta atgagtatta cggagatttt
     1261 gattgcattg ataaggaaaa aagtattttt aattttaatg taggatgtgg acgtggaggg
     1321 gacataattt caatacttat aaaagagaat tcttcaaggt tgaattgttt tgatttatat
     1381 ttacagaaag gtgatattta ttatggtaat caagcttata caaataagaa gattggtgaa
     1441 ttcttaataa tatgcgaaga tgaaattcgg ggaaatataa agttgtgctt agaaaaaggg
     1501 gcggcttgtt gtggtgaaga aggtgaaatt ttcacactta tgaaactaaa ataaaataca
     1561 gaatataaat atgttatttt atttttaaac ccctgttcat agtagtgtat ttgtaaatac
     1621 cacagtagtt cggcgtagtc tgggttggcg ttgttgttcg gcgaagtttc caaaccctga
     1681 caggaaatcg ctcgcaatcg gcaacttttc ataccacaac cgttggcagc aatattaaaa
     1741 agacatcaac aatgacaaag acaattttaa cgacattttt actttcaatc tgtctgacaa
     1801 catgtggaca gaataaaatt gaaaacaaac tgaccgaaga acaccaaaac atcaggggga
     1861 caaaagttag tttaattcca ccaaaagggt tcactgacgg actaaatttt ttaggacttc
     1921 aacaaaccga aagtggttcg agtataatga tacttgacat tccgggacct tattcagaaa
     1981 cttccgcagg aattaataag gaaaatttat taagcaaagg tgttgaactt agcaaaatag
     2041 aaaagttgac aataaatgga cttccagcta tttttgtaac gggaacacaa aatgcatacg
     2101 gaaacattta cacaaaattt attttggtat tcggtacaga aaacgaaaca ataatgatta
     2161 atggcgttca tcctgaaaac ttaaaaaagc ttggcgatga aattagaaaa tcaatgctga
     2221 ctgtttttta cgaagctgac aaaaaaatca atcagtttga cgctcttgac tacacaatag
     2281 atgtttccca aaccaaattg aattttggga aaagcatgtc aaactcttta atattttccg
     2341 ttgacggaca agtcccaaca gcttcaagcg acaaaaccaa tttgatagtg gcaaagtctt
     2401 tctctcaaat cactactgaa gataaaaaac tgttttgcat taacagacta aaacaaacgc
     2461 ctatcgagat agtgaatata gaatacacaa atgaaattac aattgacgga gtttcgggtt
     2521 acgagattta cgcaaaaggg aaaagtaaaa aaacgggcga tacagataat atttatcaag
     2581 tcattttgtt tagcgacaaa ctttattaca tactttttgg gacaacaaat gacgagactg
     2641 ataaaagcat tgaagaaata aaaaaagcag taaagacatt caaacgaaaa taaaattgat
     2701 atggaatttt caaaaattac aaacggaatc gtagaaataa ttgattcatg ggaacccaaa
     2761 ttaaccaact tgccaattga aacaattact ctgaaaagga ataatcaaaa ccgaacaatt
     2821 aaacagatcc ttggacattt aattgattct gcttcaaaca atcaccagcg aatgatcagg
     2881 ctgcaataca acaccaacct gacatttcct gattatcgac aagacaatga cctgtggata
     2941 gctcttcagg attttcagtc tgaaaattgg aataatctga ttcaactatg gaagtatttc
     3001 aacctgcaca tgattcaagt tatcaaatcg gttgaccaat ttaaacttga caactactgg
     3061 catgactttg agggaactaa agtaactctt caagagatga ttgaaggtta tctttggcat
     3121 attaatttgc atttaaacga aattcaggaa ttgattgaca aataaacgag aacaaactac
     3181 tctgttgagc gtaggttggc gtggttgttc gtcgtgttgt actgcattat aaaaaaaatc
     3241 aaagacatta tataactcaa agtatcaaca tagaaatgga aacccgtaac aacaattcga
     3301 ctccaaacat acaagagtgg tggaaaagca gacgaatgag atataacatt ggattaatcg
     3361 cttcgggagt attatcttac atactttatg ttgcacttgg gagcgtttta attgcacctt
     3421 acgactgtga atttgaaata acaattcttg gatttgtttt tcaaggattt ttcttcttaa
     3481 ttactatact aattgcaaac atattttata accttggagc ttttacagac aaacactata
     3541 ataaaaagaa tgaagagaaa ttccgacaaa gattgtataa tttggggttt tggttttcat
     3601 tcggacttcc tttcctagtc ccgttattga ttattgtaaa gtattttact tgttatgcaa
     3661 acatgaaata aaatgaagaa caacccttag ttcggcttag tctgggttgg cgtgattgtt
     3721 cgtcgaagtt tcttgcttcg tttccgatta atcgtaaacg aagcagaagc catacgccga
     3781 acaacagagt gccagcatta cactacaggg aacgattgcg gatttgtgtg ccaataaaaa
     3841 ataaagttct ttgctcccgt gctatcatcg cgttggaaag atgcaaataa aaaagaacat
     3901 acccgcacgg ttgcggaaaa acaacactcc ttgcctgatt ttcaatactt tggataggtt
     3961 gtttgttgat ataataattt atactacatc aataaaacat atccgcaagg atgaagatgc
     4021 aacttatcgt ttttcttcaa caaaagtgat gcttccatgt taattttgtg cgattttgct
     4081 tcattgtaat atttttattc tcaactatta atgataattt attatttttg cgtgaagaaa
     4141 ttcttcggaa attctcaatt tgtaaatgaa tataatgaaa gatataatac ctgctgttga
     4201 ccgtacactt ctgaaatcgg agctcacacc cgatatatac ctcagaccaa ccaacaaagg
     4261 tcacaatgag atatacatta ccacagctgc caaatcgccg aatgtgatgc gcgaaatagg
     4321 ccgcttgcgt gaactggctt tccgttcgtg gggagggggc agcggcaatg agatggatac
     4381 cgacgaatat gattttctga aaaagcccta caaacaactt attgtgtggg acccggaagc
     4441 cgaagaaatt gtcggcggat atcgttatct ctcaggacgt gatgttacaa tggacaaaga
     4501 tggtcagcca cagtttgtga tgtcgcacct tttccgtttc agcgacacat ttatcagaga
     4561 ttacctcccc tataccatag aactgggacg agcctttgtg caacccgatt atcagactgc
     4621 gaaaatggga acaaaaagcc tcttcgcgct cgataacctg tgggacggac tgggagcctt
     4681 gatccataag gagaaaaaga acctgtattt tgtcggaaaa gttaccattt acgataccta
     4741 tccggtgctt gcccgcgaac tgctttatga atacatgttc ctgcactgcc ccgataaaga
     4801 tgaacttatc cggcctaagc aggaagtacc tgtaagcgaa acgggcaaac agaaagcgcg
     4861 tgaaatattc acgggaaacg acgcaaagga agattacaaa gcactgcaga aagccattcg
     4921 ccacgaagga accaccattc cgcccatgtt taatgcctac atcggcctta ccgacaccct
     4981 gcgcatgttc ggttcaataa ttgatcccga tttcagcggt gtgtacgaaa gcggtatcat
     5041 gctcactatc ggcgacctta tcgattccaa acgccagcgc tacatcgatc catatatcgc
     5101 atatctcaaa caactgctga acgaaaaacg ggctgcacac cgcgcagcta aaatcgcgaa
     5161 aggaaaggat gacacacctt caaaaattaa gcagctagca caggaaatca tagaaaaaac
     5221 gaaaagagat aaatagtatc tcgtataatt tgcttcattc caaagaattc cattttgttg
     5281 ccggaattta gattttaaga atatttactt cataatctct cagcctgtaa aaatatatac
     5341 agggtgggag tgtttatatg catcactgac gggtatgagc cagtttttta tcccatgcca
     5401 atttaactgt catattgtgt ctattaacac acctgatata taaaaaaaat atcaaaaata
     5461 tttatggtga aaattcaaaa ataaagcgtg acgactgcag attgtcacgc tttaattttt
     5521 cacattgaca tgtttacaat caggttcatt tcatgtcagt gaatttctga aagattattt
     5581 tttttgaagc aaaatttata tgtaatttcg caacaccaaa tattaatctg ataatggatg
     5641 aaataaaatt caaacataca atacccattc agctcaggtt caacgacttt gatgccctgg
     5701 ggcacgtaaa caactcggtt tatttttcat tttatgacct tggaaaaaca gaatatttcc
     5761 gggaagtcat acccgaaatg atctccaacc aagaagttgg agtggtgata gcaaacattc
     5821 aggtaagctt tttgttgccc gtgtttcctg gcgaaaacgt agctgtacag acggcagtta
     5881 ccgaaatcgg taataaaagt ttcaaacttc tgcaacaact cattgattgt gaaaccaacg
     5941 aagtgaaatg catctgcgac accgtaatgg ttggttatga tgcaaaaacc aaagtcacac
     6001 ggacaatatc tgatgaatgg agaaaagcaa tcaacgatta cgagggtaga ggcgatctga
     6061 gcaaataaaa aaaggattgg gtaaaaaaac cgatccttca ttttattatt tatggttgta
     6121 acctgttgat tggtgaatta aacaggcttt actctgatta tctttttatc cctcagcagc
     6181 ggatttaatt ttccggacac aagttccctg aaataagggc tttcggtatg tttggcgtga
     6241 gctgtcatat tctcaaaaca ttcgtacaga tataactttt cctgattctc ttcactttgc
     6301 agtatttcaa aggcatagca tccctgctca ccgagcgcat taatttgcag ttctttcagc
     6361 aaatcagtaa cttcctgtaa atgatgagcc tccacatcga attctttcag aactgcgtag
     6421 ggcccggctt ttatcgtaaa attattctcc acggattata atcttattaa gttattgact
     6481 tattgagtta ttggctaata cttcattcaa ataaactaaa attcggatgt aaaatggaaa
     6541 cgaatgtttt tgaaatcgtt ctgcgccatc tgcagtacat agttggagtc agccaggaat
     6601 acatcgcggc catccttatc ctttgccata tactgtactt tacgcttttt gaagttttct
     6661 aattccacag tgtcatccga ttcaatccaa caagccttga aaagtgaaat aggttcccag
     6721 cggcagctgg cattatactc atgcagcaga cggtattgta tcacttcaaa ctgcagttgt
     6781 cctacagttc ctacaattct acggttgttg aactgattga caaaaagctg tgccacacct
     6841 tcgtccatca actgattaat tcctttatcc agctgtttgg ctttcattgg gtcggaattt
     6901 tccagataca taaacatctc gggtgaaaaa ctcggtatcc ctttaaagtg taattcctca
     6961 ccaccggtta gggtgtcgcc tatcttaaag ttgccggtat cgggcaaacc aacaatatca
     7021 ccggcaaaag cctcatctac agtttctttt ttctgtgcca taaaagccgt tggacttgaa
     7081 aatcgcatca tcttaccatg gcgaatatgc ttgtaattga cattgcgttc gaaccgtccc
     7141 gagcaaattt tcacgaaagc aatacaactg cggtgattgg gatccatatt ggcgtgtatc
     7201 ttgaagacaa atcccgaaaa tgcttcctcg taaggatcga ccacccgttc gagggtcttt
     7261 accggtcgag gtgatggtgc tacttccaca aaacaatcga gcaattcctt cactccgaaa
     7321 ttattcaacg cgctcccgaa aaatacaggt gcaagatgtc cttgcatata cagatcacta
     7381 ttaaaagcgg aataaacgcc attgatcagt tccatatctt cacgcagttt atcggccggt
     7441 tttttcccta ccagatgatc gagttcctgg ctgtcaatat ccgctagctc caccgactcg
     7501 ctgataagct gtttattcgg cgtaaacaac tgcaagttct gtttatagat attgtaaact
     7561 cctttgaatg tggctcctat gcctattggc cagcttagcg gacgaacatt gatacccagt
     7621 tcagcttcca gttcatccat cagatcgaaa gggtcgcgtc cttcacggtc cattttattc
     7681 acaaatacaa tgataggcgt gttgcgcatc cggcaaacgt tcatcagttt cctggtttgt
     7741 gcttccaccc ctttggcagc atccaccacg atgattacgc tgtcaacagc tgtcagagta
     7801 cggaaagtgt cttctgcaaa gtcctggtga ccgggcgtat cgagaatatt aattttgaaa
     7861 tcgcgatact cgaaacccat aaccgaagtt gccaccgaaa ttccacgctg tttttcaatt
     7921 tccatccagt cggatgtggc tgtttttcgg attttatttg atttgacagc gcctgcaata
     7981 tggatagcac cgccgaaaag taaaagtttc tcggtaagcg ttgttttccc tgcatcgggg
     8041 tgactgataa tggcaaatgt tctgcgtctt tttatttcgg attgtaaact cattgattga
     8101 ttgttgtaac gttgtattgt tgataaattg ttccgggtag tctcaaaaaa tttcgaacag
     8161 acagggaaag ttatcgttga tttctaatct gtttttcggt cggcaaaggt agtgtatttc
     8221 acgtaaatac acaaaaaaaa ccaatcgctg aggattggtt ttttgttaaa ggttcgggat
     8281 tttctcagag ttttataccc agtgtaacca caatattgtt gttttttgta tttacagctg
     8341 caggagtgac aatatttgtt attcggttgt tttggtagcg gagtatttgg tagggataca
     8401 tatcctcatc cagcatcttc tgcgcatagg caaaatcaat aaaccagccc gcagcacggt
     8461 atccggctcc aaaagtaagg taacgtgtat ccatctgatt gaaatattcc gtatttgtat
     8521 tggcggaatt cagtttcagc gatttaccct gcgcataatc ggtttgattt gcacttttca
     8581 tcagtgcata accggcacgc agagcaaaac ttgggctcag tttatactcg gcacccactt
     8641 tcagggtctg tccggcttta taaacatttg gcatgttctg attttcatct ttgtaatatt
     8701 gagtatcccc gttttccagt ccaagccgca tacccgggta gtttacatag tcatattcga
     8761 ctgatataag tcccttttta ccaaatagat aagctgcgct tgcctgagcc tgaaggggac
     8821 cggtaatttt gtaatcatga ctgtaaccct gcagcggcag gtaagaatcg ttaatttccg
     8881 aagtcttgaa attcaacaca ttggtatatt cggatatgga ataaaacgta ggggtatgga
     8941 aagacacacc cagtcttaaa ttatttgttg ggagataaat ggctcccagt ttcagattaa
     9001 agccaacacc agtttgcgac attgcagagg taaggtcaaa gtagccgcct tttgcaaagt
     9061 cctctgcaat agttgaatgc aggttgtaag ataaatcctg catattcaca ttggctccga
     9121 taaagagatt gttattgaag ttaccacccc atccaaatcc gtattcgctc aaagcacctg
     9181 tttcaaagag atatgtagat ggagccacag tttcgccttc gtccagaatg gatgaccagg
     9241 tattgccttc ctggggatta atcaggtaat tctgatatcc aagtaccgaa agccatgcat
     9301 tatcaggatt ttcatacgga tctttgatag tagagttttc ctgcagatca gcttctgaat
     9361 atctttttga aaatgcagcc agaaagtcgg tgaatgaatt atcgctacct ggactagcag
     9421 cacttacttt ccggttgaaa tttcgcaggc gattgaaagt aaatgaaaaa ttagagctca
     9481 tcaaaccgct gttcttttca ctccagctgg gcattgccag tacgtacgaa aaactgttaa
     9541 gatttacttt aagcatatct tccttcgatg tcatgccact ccataccgaa ccggtgttct
     9601 gaatcattgt attgagtgtc ccggcaattt cggaaccacg gtataccccc agtccggcgg
     9661 gattatcctt caatgccgtg gcatcgccac ccagtgctcc gaaagcacct cccattccca
     9721 tgtatcgggc agatccgctt agatcagtct gcgtcagttt gaaagcatcg tattcagttt
     9781 gtgcaaaagc taatgtgctt acaaaagcag ccaatacaac tataatattt tttttcatat
     9841 tcgtaatatc tattgattga tttgatttga attctgttta tttccaaaaa cacttcactt
     9901 tttatctccg tccgccactg ccggaacgac tgctgccgga actactgctg ctactactgc
     9961 tactacttcc actgctatac gagcccgaac ttccggatga atatgaacca gacgatgtgc
    10021 taccacttga ataagtgctg ctgttagaac ttctgctatt atatgaaggt gaatacgaac
    10081 tgcttgatcg gcttgacgat gaaccgctat tgtatgaacg gcttccagaa cttcggctgt
    10141 ctgagcctga gcggttgatt accgttgaag atccggagga acttctggtt tcgtttgaat
    10201 aacgtgttgc attgctgttt cgtgttgcat tccattgatc ggacgaccgg acagcgcctg
    10261 tacgggttgt tgtgttcaca ttaggtgaat atgaattgcg ttcggaggag gtgtacgaac
    10321 ttctggtctg aacaccgcga ccggatgtag tgttctgcat accgcgacct gatgttgata
    10381 aattatttga ttcaaacctg tttctttgtg taccctgacg gctgactgta gtgtagagat
    10441 tactgcctga agttcgaccc gaacctaata cagcctgctc gcgtgtaact gaagttctgc
    10501 ttgttggacg tgttgattca aatcttccta cgcgtccgtt ctcacgggta ttgtgattat
    10561 tgccggaata atacccgtta ccagaatacg gataacccaa gccaccataa ccataatagc
    10621 caccataata cggataaccc cagccataat aaccgccgta atagggataa ccccagtcat
    10681 aataaccacc ccatccatat ccgtatcctc cgtaccatga actccatgga gaataccacg
    10741 gatcgtaata tccccatccg aaagaagaat acagactgtt tctccagccc cagctgctgt
    10801 agccgtatgg gaaatactga taattaaacc agtaagggtt ggtccaggta ggtgttatgg
    10861 tagcatatat tccatctacg tacacattcc agtcattttc atccagaaaa tagatgtcgt
    10921 tgtaaccggg atctgcaatt gtaattgtat atcggggatt atggaaacgg cgaatacgtt
    10981 ctgcatattc caaatcttgt tgcgatcctt taaatccgcg caggtattca ccttccgttt
    11041 caacggtatc aaccgtttca atagaatcgt ttactgtatc gtccatcaca taaacggtat
    11101 cagtcactgc tgtagtcctt ttaccattac ggtcaatgaa gattatttct cttgcaccgt
    11161 ttttataagc tggcttttca cgaacggttt ttttagctgt ttgtacttcc ttaaccatac
    11221 cggcgtcatt gggagtgaca tagatatcat ctatgatttt ttttgactgt gcaaaaccag
    11281 atgaaaccag cgcaatcaaa aaactaatta ataaactaaa gcgtttcatt ttataatatt
    11341 attaaaggtt gagaagtatt ataatgtatc gaattacaaa ataaaaatca caaaaattgt
    11401 gccataacat taagaagcta tgactataat ataaagattt acaaaaggca caaatgtact
    11461 taaatttggc aacctattca acttaatttt gtacttttgt tcactggaaa ttcaattttt
    11521 tcagacaata aacaaagctt atgaaataca ctgccattca ttttacgttt gatacttctg
    11581 ttgaatacac agcggacatt cttgcctcca tgctgggcga taccggcttt gagagtttcg
    11641 taaacacgga taaagggcta gatgcttaca ttccttcaaa actctacgat gaaaacgccc
    11701 tgaaagttac cctaaagaac tttccctttc cagaagaaat agattttgaa aaagaagaaa
    11761 tggaagataa aaactggaac gaagaatggg aaaagtactt tttcgagcca attatcatca
    11821 atgatgagtg tattatacac agttcctttc ataaagacat accaaagttg aagtacgata
    11881 ttattattga ccccaaaatg gctttcggaa caggtcatca cgaaacaaca agtctgatga
    11941 ttggtgagat tctgaacatg gatcttgccg gaaaatcggt gcttgatatg ggatgtggca
    12001 cttcggttct tgccatactg gcatcaatgg gtggtgcagg tgatcttacc gccattgaca
    12061 ttgacgactg gtgcgttgac aattctttgg aaaacataca attgaacaat gtcagtggca
    12121 ttgatgtact gctgggagga gctgaattgc tcgaaggcct tcatttcgat atcgtattgg
    12181 caaatataaa ccgtaatatt ctccttaacg atatgcaatc atatgccaac acactgcctg
    12241 cagggggtga actgtatatg agcggttttt acactgagga tgtcccggtg attgaaacca
    12301 aagcccgcga acttggtttt gagatgcttt attctaagca aaaaaacaac tgggcagttg
    12361 taagaatgct taagaaattt tgatttttga tgcagcttaa caacattcag agattctgtt
    12421 ggaactgttg gagcaccttt atcacattta ataacaggat atcagcctta taattctgtc
    12481 atatattttg gtagaatgac gtggaaaaca gttccctcat ctattttggt ctcaaatgta
    12541 atctgccctc cagccatacg aatcatattc tgacttatag ataatccaag acccattcct
    12601 gtagattttg tagtaaagtt gggcgtaaaa agtttatctt ttatgtcgtc ggaaattcct
    12661 tttccattat cgcggatgtg tacaaaaacg ttttcaggag ttgtttctac cgatacctga
    12721 attttccctt cacgcccggt aggaatagat tgtatcgcgt ttttcaacag attgttgaac
    12781 acctgtatga gttgttctcg gtctgctatt acaaaagcat cccgaacatc gctccggaaa
    12841 ataattcttg tcccatcgtg gttattggaa aacaattcta caaccatgcc aacgattttc
    12901 gatacatcta ctttatccgg tttagcctca ggcaaacgcg caaaagtcga gaatgttcct
    12961 gcaatctgcg ataaattttc aatttgctca ataagcgtta cagtcgattt ctcaaaatac
    13021 tcatcaaaac gattgtctcc cattttttta gtacgttgca gttgctgtat ggtaagtttc
    13081 ataggagtga gcggattatt gatttcatgc gcaacctgtc tcgccatagt tttccaggct
    13141 gactcgcgtt ctgattttgc cagtaaccgg gcactttctt caagttcatc taccgtttta
    13201 ttatattgta ttaccaactg accaatttcg tcattcggtt tatactcaat ttttttattc
    13261 cgctgcccca gccggatgtc tttcaagctt ctttcaagca ttgcaagcgg ctcggagagc
    13321 tttttgttga taaccaagct taaaaatatt aacaggatag tgattaccag atatatatga
    13381 acaataacgg cgagaaattc ctgaatttca gcctgaatct gatcattgct gaaatattgc
    13441 ggcactgcaa tatagcctat ttgaagaaaa tcaccattgt agaagtcggt ataagctgta
    13501 aggtattcca gttctccgat aaattcataa ttattcatat tgggattttt ggagaagaat
    13561 ggtaagggag aaattctctt actgatcaga ccgcgtgaaa aaatactggg ttgtgaactt
    13621 gcaataagct ttccctcgtt gtcgtaaacg tgaatatcat tttgataagt ataagaaagg
    13681 tcctgtaaga aaagattaag agaattctcc atggaagaat caattttttc tgtccagtaa
    13741 tatttttcct gtaaagcagt ctgaatataa tttttagttc gttccagatt aatacgctgt
    13801 tcgtttcggt acttttgttg gatatagttt atggatacat aaaaaataga aacaaaactt
    13861 atgatcagca gtacgacaaa tgaataaagg tatcttgatg tcaaccggaa tggattggaa
    13921 gtttttttgt gaatcaactt ataaatccag aaaatcagaa atgctaccag taaataaacg
    13981 acgaaagtat ataaaacata aagcagatac ccccagatac tgtttgtgtt ttcttcagac
    14041 aatatgaaat aattatagat attggggaca tatatataat gccggtaact tccataatcc
    14101 tgggtataaa aattcaaagg ttttttatca atccattttc catctcgggg atagcggaat
    14161 tttccggaag tgaatgctag ttcgtgataa gtataacgcg cactcgagag ttcaagttgt
    14221 tgctgcagac ttggcgttcc ttcaaccagc aatccgggga aactataact tttataatac
    14281 ttcttcggat aaaattccat atagaaattc acttttttcc cgcccgactg ttctagctta
    14341 aaagcgccca aaaatgccat atccgacgat ttgctattca tcacaaagaa gttacttgat
    14401 tttatctgat tgccgtatga atttattgtt tcctgatatg tttgatccag aaccgtaccc
    14461 ggcaaagttg caaacaatct catttcatat tttgaccaga aaccccgaag ataggcttta
    14521 tttaggtaat cattggcttt ctgaacagaa tctggatact gcatcagatg tctcacaact
    14581 tcatctttct tcacttccct gtcaatgtca accagtatga gctctgcgat tttatcttca
    14641 tcagcattat cattcagata gtgattttcg gccagcgttt tatatttttc aaatttcttg
    14701 tccttgttca tgttaataaa attccaggta acaaagaggg tgatgaaaaa caaccagata
    14761 gccacaaacc atttattatc cattaaacct ctgtacagca aaggaagata taaaattata
    14821 ttaggaataa gaaaagaaac agcggcgtct aacccatatg ggcgaagatt aaaaaacagg
    14881 agaacacaaa aaaacaatgt cacaggaata tcgatcaaca gcaaacctct cagtcctttt
    14941 tttcgtaaaa taatccggtg aattttcagg tacaaagaca tataactaat tcccaagaaa
    15001 aacaacagga agtggttcca aaccgacacc caattgaagt cagttaattt caggatattc
    15061 agttcggtgg tagaattaaa tactagcccg accataaagt tgaaaatcag cataaaatat
    15121 gctccgggaa tcaacagcaa aacaaccgct ttcagggtga aatatttatc ttttcgcaag
    15181 tttctatcgg tatgaaaaca gaaaagatag atagtagaac aaatatatga agtcagaaaa
    15241 ctcaaatggg ttaacgatgc caggattgaa ttggttgcat aatgatacgg tgtgaagatt
    15301 ttatttgaaa aaaaggtggt aggcagatta aacttcaaac aaataatcac aaacaatgtc
    15361 ataatcgaag ttacctccag gaattccttc caggtaaaca gtttcttacc tatcagcaaa
    15421 gggaaagtgg caaaaaggta aaacaacaaa aagaatgctg ccagaaagaa atacatcgcg
    15481 gcaatggccc agttttcatt ataaacaggc tgatcgggaa gtttcagggt aaatagataa
    15541 tcgttttgat tgctgaatat tgcatattta tctgctggtt tattcagcac aattccaatt
    15601 tccttattta gtttgaaacc ttcaaaatac ttattggtaa gctccttatt ttcgtaggaa
    15661 taattatatt ttacaggtat gtaagcaacg atattaaact cactaatctt tttcgatttt
    15721 gcaatagtat gcatattggg catcaataca tattcccagg ctgtattctg cagatttttc
    15781 tggctggtaa tattattcga ccagaaaatc agttctccat tccggtaaac aagataggtg
    15841 tttgctaatt tctgaaaaga aatcaggttc agtgaatcga gcgattttcg ttgcagaagg
    15901 ccctcaatca ccttcatttt ttcttccgct ttccgttgtt ttacactgat agcatacctt
    15961 acatcattta tgggaatggc atttgccggt gattgtttgt aaaaccactg gcatacaact
    16021 ccaagcagtg caaatacaca gagtagaatc aacgcgacac tgtttctgtt taaaattctt
    16081 tgcttttcag cattatttct attcatgatg ataaggttct ccctttatga tggtcattgc
    16141 acggtaaagt tgctctacaa acaacaaacg tatcatctga tgtgaaaagg tcatggatga
    16201 aagcgaaatc atcgaatttg cacgtttata tacttgttca gaaaatccat acggtccacc
    16261 aataacaaaa accgtgcatt tattggatgt cagcatcagg tttgaaatca attccgaaaa
    16321 acgaaccgat gtaaactgtt ttcctttttc gtcaagcaat accacatggt ccggattttc
    16381 gagtttaccc agaatctgtt caccttcctt ctctttttgc aattcaacac tcaagctttt
    16441 agcgtttttc aattcaggta caacaaccag ctcaaagccg atgtaatgtt tcaagcggcc
    16501 tgcataacca tctatcagtt ctgtaagtcg gggatctgtg gtttttccca ccattaacaa
    16561 ttttattttc acctgaaatt attttttaaa aagtttggcc tgttatttgt tagataaata
    16621 ataaaaagac ttctaagaca cagatatttt aataactttg ctgccggatt tcttttacaa
    16681 atgcaaatat tgcacaaatt tagaagaaaa aacgttaccc ggctatttat ttatgaaaag
    16741 aaaaaaagaa aatgaagact tataaattta ttgcgttaat cgcatttttc tcactaataa
    16801 catcacaaaa cattacagcc cagaatgttg atgtgccgaa tgaaattacg gttgcactta
    16861 acagcgggga tgccggcaaa ctttcagcct ggttaaataa taatgtggaa ctggttattg
    16921 gcgataaaaa tgatgtttat agcaagcagc aggctattgg tattatttcc gatttcttca
    16981 aaaataattc agttctggga tttcaagtcc ttcacaaggg taatcgggaa gccgccagtt
    17041 ttgtaatcgg tacgcttaaa acttcaagca ggaattacag agtatacgtt ctgacccgaa
    17101 gaacaggaaa acaatcctta atacagcaat tacgaataga aacaaataat gaataaagat
    17161 tttatcaaaa tgatcccggc atggtttgcc gaagacattg gcgatggtga tcatacaaca
    17221 ctatcatgta ttccgcccga tgcaatgggc aaatcgcaac ttataattaa agaggacggt
    17281 ataattgccg gagtggaagt tgcccgcgag attttcaaag cttttgaccc ggaattaaaa
    17341 atgacagttt tcattcagga cggttcggag gtaaaatatg gagatattgc ctttgtggtt
    17401 gaaggaaaaa ttcagtcatt actccaaact gaaagactta tgctgaacat catgcaacga
    17461 ctgagcggag ttgcaacccg tacgcatgaa tatatgcagt tgctcaaagg tacacgtacc
    17521 agcgtgctcg acacccgcaa aaccactccg ggacttcgca tgctggagaa agaagccgtt
    17581 aaactcggag gcggcaccaa tcaccgcatc ggactgtatg atatgatcct gctgaaagat
    17641 aaccacgtgg attttgcagg aggtatcgaa caggcaatta cccgcaccaa agaatacctc
    17701 aaggaaaaag gcaaaaatct taaaatagag attgaggttc gtaactttga tgaactgcaa
    17761 caggtgctca atatcggtgg tattgatcgg attatgctgg acaatttttc agttgaaaac
    17821 acacgaaaag cagttgaaat gattggaggc aaatatgaaa ctgaatcatc aggaggtatc
    17881 accaaagaaa cattgcgcga ttatgccgaa tgcggagtgg attatatctc ggtgggtgca
    17941 ctcacccact ccattaaaag tctggatatg agctttaagg cagttgaata aataaccaaa
    18001 tatgcaactc tgattaataa accgttttca cctaaaggaa acggtttttt ttgtcgtctg
    18061 aaaggatatt ttatttcata aacgttataa ttatagcaga aaatattttt ttcaaataat
    18121 taaaaatcag gaggcaggaa aaatggatca gaacaaacca ttagtaataa ccattagccg
    18181 ccagcttgga agcggaggtg cttatatcgg tcatcagctg gcaaaaagac ttagcatgta
    18241 ttatgctgac catgaaataa ttacaaaaac agcacaggaa cttagaatgc ttgaaaaaga
    18301 cctggaacaa caagaagaaa agttcgaatc cttctggaaa actttcttgc gttacaatgt
    18361 atcagctccg gaggtttaca tcccgtcttc ctataatttt ttcccaacta cccaacagct
    18421 ttttgaaacc gaatccgatg taattaaaca tatcgccgac gaaagttcct cagttattat
    18481 cggtcgttgt ggtttccaca ttctgcgcga ccatcctaac aaagtcagtg tattgattta
    18541 ctgcgacctc gaaaaacgcg ttcagcggat caaggatatc cgccatatga cggaagaaga
    18601 agctcgaaaa accgtactgc aaaacgataa ggagcgtgaa ctctacatca acaatttcag
    18661 caaaaagaaa tggatggatg cccgcaattt tgatatttgt attgatacag gaaaaatcag
    18721 tttcgacaac agtgtggaac ttatcatcaa ctatctcaat ctgaggaaat aacatatata
    18781 cattccggtt tgaacccagg tgaaattttt attaaaaaaa cgctgcgtag ataaaaaata
    18841 tgttttcagg actatgaaca attgagatac ccgattttgt tttttaactg aaaaataacg
    18901 atatttgcaa agagttacaa tttatgtgta actctttatt tatttaaatc accacataat
    18961 atataatatg aaaatagcta ttataggtac cggctatgtc gggctcgttt cgggaacctg
    19021 ctttgccgag atgggcgtgg atgtcacctg cgtggatgtg gacagtacaa aaatcgacaa
    19081 tctgcacaaa ggcattattc cgatttacga gccgggactg gaagatatgg ttaaccgcaa
    19141 cgtcaaagct gaaagactgc atttcagtac caggttggat gatgtattgg atgatgtgga
    19201 agttattttc agcgctgtag gtactcctcc caacgaagac ggaagcgccg acctgaaata
    19261 tgtgttacag gttgcccgtg aagtgggaca gaaaatgaac aaatacgtat tactggtaac
    19321 aaaaagtacc gttccggtgg gcactgccag gctggtgaaa caagccattc aggaagaact
    19381 ggacaaacgc ggcgtcagca ttccgtttga cgtagcttcc aatcccgaat ttctgaaaga
    19441 aggcgatgca atcaacgact tcatgagccc cgaccgcgtg gtggtgggca ccgaaagtga
    19501 aaaagcaaaa gaactgatta ccagacttta tcgtcctttt ctgctgaata atttcagggt
    19561 aatcttcatg gacattcctt ctgccgaaat gaccaaatat gctgccaact ccatgctggc
    19621 tacccgtatc agttttatga acgacattgc caacctgtgt gaaattattg gcgccgacat
    19681 caatatggta cgcaaaggga tcggcactga cgttcgcatc gggagcaaat ttctatatgc
    19741 cggttcggga tatggcggat catgcttccc caaagatgtg caggcgctta tacggacagc
    19801 tgaggaaaac ggctatgaac tcaaactgct taaagccgtg gaagaagtca acaatacgca
    19861 gaaagaggta cttttccgta aactggcaaa acgctgggga cacgacatga gcggaaaaac
    19921 tgtagcagtg tggggactgg cgttcaagcc aaacaccgat gatatccgtc aggcacccgc
    19981 gtttgacatt atccggaaat tgttgctggc aggcgcgact gtccgctgct ttgacccgat
    20041 tgccattcct gcctcaaaat cttacctgga atatctcgaa aaggataata atacaacaga
    20101 aaaaccggac tattcaaaag tatattttgc caatgatatt tatgatgcca cttttgatgc
    20161 cgatgccctg ctgctggtta ccgaatggaa agaattcaga atgcccagtt ggaatgtggt
    20221 taaaaaatcg ttgaaaaatc cgctggtact cgacggcaga aatatctacg ataaaactga
    20281 aatgaaagaa ctcggctttg aatatgaatg tatcggataa aaaagccgca ggatctatcc
    20341 tcaatgaaat aaaacattaa aacagaacag gattgcaaaa actgcaatcc tgttctgttt
    20401 taaaacatcc gtaatatact gatatcatat ttttacttgt aactggttta cttatttacc
    20461 ctgaatttct gatttccgtt tttcagaaaa tcaacgattt gctgagcggc agctataccg
    20521 gcatttacat tggcttcggc agtctgagcg cccattttct tggcagtggc aaagtaacgg
    20581 tcaccgactt ttaccttaaa ttcctcgtcg gtatccggag taacatctgt aacgtatttg
    20641 aatttcggat tatcgaccaa atgctgcagc aaatcggctt catgcaccac ttccttacga
    20701 gctgtattaa caagtacggc atcttttggc atcaactcca gcaattcttt attaataacc
    20761 tgttttgttt cgggagttaa cggtatatgt acagaaatca tttcgcaact ttgataaagt
    20821 gtcttaaccg aacaggtagg attaacacct tcagccttta tatcctctgg acgacaaaaa
    20881 gcatcataag ccgaaacttc cattccaaat cctttagcaa tgcgggcaac attcctgcca
    20941 acatttccat aggcatgaat gccaagcttt ttacccatca attcagtacc ggaagtcccg
    21001 ttgtaaaaat tgcgaacagc aaaaaccagc aatccgaaaa caagttcggc cacagcattt
    21061 gaattctgac cgggagtatt catgacacat attccggcgc gagtagctgc ttccagatcc
    21121 acattgtcgt atcctgcacc ggcacgaaca acaatcttca gttttttccc tgcattgatg
    21181 acttcctcat ccacaatatc gctgcggata atcacagcgt cagcatcggc aatggcatcc
    21241 agcaactgct gtttttcggt gtatttttca agcagagcca gttcgtaacc aactccttct
    21301 atttcgttgc gaataccatc caccgctact ttggcaaatg gtttatcggt tgcaataagt
    21361 actttcatag taattaaaat ctgtggcccc ctaaccccct aaagggggaa ttagcgagca
    21421 tttttttatg taatttatgt aaattaattt caataaatcc ttcaaatgtg aaggaaacgt
    21481 ctattttgca caatcagttc cccccttcag agcctgtccc gaacttgttt cgggagggtc
    21541 aggggggcag gttttctaat tcattttctc gaattccttc atgcaggcta ccaatgcttc
    21601 gactgcttcc agcggacagg cgttatagat agaagctctg aaaccgccca ccgaacggtg
    21661 acctttgata cctaccattc ctctttcctt ggcaaaattc atgaagtcgg cttctttgtc
    21721 cttatactct tcagccatca cgtaacatac attcataatc gaacggtctt ctttaacagc
    21781 tgtacctacg aacattttgt tgcggtcaat ttcgtcatac aacagattgg ctttctgaag
    21841 atttatttta tacatggctt ccacgcctcc aagatctttc acccatttga gtgtttcgtg
    21901 catgacgaaa atggagaata ccggcggagt attaaacatg gagttatctt tggctgtttc
    21961 gccgatgtgt gtccggtagt ccaccatagt ttggagtttt ctgtccattt tgccgagcag
    22021 gtccttgcgt acaataacga atgcaacacc ggccggacct acatttttct gagcgccacc
    22081 gtaaatcagc gcatatttgc tgacatctac cggacgcgac atgatatcag acgacatatc
    22141 ggcaaccaaa ggaattgggc tgttaatatc ttcatgaatt tcggttccgt aaatggtatt
    22201 attggtggtg atgtggaaat aatcagcatc cgttgggatg gtgtaacttt ttggtatgta
    22261 ggtataattt ttatcttcgg aagatgcaac cacctcaact tcgccataaa attttgcttc
    22321 tttgatggct ttcttagccc atacacctgt ttgcagataa gctgcttttt tgcctaaaag
    22381 attggcagga acatagaaaa actgtgtgga cgcgccacct cccagaaaca aaactgcata
    22441 ctcttcaggg atattgagca gttctctcca gagttgctct gtttcagcca taatccgttc
    22501 ccagcccggc gtacggtgag aaatttcaag aatacctatt cctgtattat caaaatcgcg
    22561 aatagcttca atagctgatt caatagccga cttgggcaaa acacatggtc ctgcattaaa
    22621 attatacttt ttcatacggt aataatttgt tttttaaagg tagtatggaa ctcgcctcaa
    22681 atgtcgggac ttgtttcata tatcttcgat ttttaatgaa tttaagttct gaagcagatg
    22741 acaaaattac gtaatttttc catatttact gaagataaaa cagaatattt aggtaaaaag
    22801 ccggttttta cataaacata cacagcaatt tcatttttga aaaacaaaaa gcagaaatct
    22861 aactgaattc tgcttttctc tgtcaattta tttcgtttgt tgtttgataa atttcaacat
    22921 ctcattcagc gcgagcggcg agaatgtctg ttctattacc gcgtattccg atggcgatcc
    22981 ggtagtacat tcctgaaata aatgatttaa ccccggcaat tcaatagctg tcagtctttt
    23041 atttccgctt tcgccaaatg ctttgcggat gagagtcaga ttttcagcag gcggaacctg
    23101 caagtctttt gatccgttga gtgccagcgt cggacatttt actttcttaa gcgttggaaa
    23161 cggatcatac tttataaaat actgcatcca gggtgaagcg agctgatcgg tatatccctt
    23221 agcctgcctg tcctgttgtt ccagtgtcgg gttttcgcca agggtgggat catctttcag
    23281 ggtggtcagc aaatagtcag tcaggtcagt tttgagtttt gccgtatcat tcgaatgaag
    23341 caccagatcg tatgcccctg aatttagtct cagtatgttg gcaagttcgg tttcggtcat
    23401 tccggaagct ttggctattg cagcggtctg caattcaagc aattctttcc cccgcaaccc
    23461 gggacctgca agcatcacaa taaaagcaac tttcctgttg cgggcagcta ccattggtgc
    23521 aataattcct ccttcactat gaccggcaag cccaattttc tttttattta tttctttgcg
    23581 ggtcaacaga taactgactg cggcttcagc atcgtttgcc aaaccggttg agttcgcttt
    23641 ctgaaaatcg cctgtggact gagctgttcc gcggtcgtcg taacgaagta cagctatgcc
    23701 gtttcgtgtc aggaaatcag caataaccag gaatggcttg tgacccatca gttcttcatc
    23761 cctgttttga gctccactgc cgctgatgag tactactacc ggatattttc cctccttttt
    23821 cggcattgta agcgttccgg ccagcgtatc accgtctatt gaattgataa acttcacgtc
    23881 ttcggagaga taagggaaag gcgcttttgg ttcctgagga cgaagcactt tttccttttc
    23941 aattttagtt gtcgtcaaat caagcggcag tgacataccc atttgcgcaa aagtaccttt
    24001 gataaccttg tcttctccaa gtacaccggt gtaagttacg cctatcattt ttacctcaaa
    24061 tttcaaggta tcgttagtga aagtcaccaa atctaccgga atgcctttcg ctccctgatc
    24121 gggactgtcc atggtggctt tgtagccgtt ttcagcttgc tgaatattaa tcaccaggcg
    24181 aatttgctga ccctgtactt tgagcaatcc gttccacgag ccctccggac tttgtgaaaa
    24241 aagcactgct gaaacaaaca gggcaaaaat aaagacgatt tgttttttca ttggatgtgt
    24301 gttttttttc agattataat tgaaatcaag attttcgaaa taacttcggc aacaaacaac
    24361 cctgcaataa atcctacaat cattttcgga cctttagcac cggttgaaac tttccaggca
    24421 ttgtacataa gcgctatgac ccacaccata accggaatcg tcaggacgat gaacactaaa
    24481 aaaccgggat tggtgaaaac ccgataagga ttggccagta aatcagctgt ttcgggtaca
    24541 gtcacaaaca atccggcgag cgagagcacg atgtaaggtg cgcgggcaag tgtcattgtc
    24601 ccgagcaaat caataaacct gaaatttttc gtaacgaaca atccggctac atacatgaca
    24661 atactcatac acaccacatc gatgacaagt attataagcg cctgataata tgtcacatca
    24721 ttgctcaggt gcgcgtcgat tgcaccatcg aacaccagac cggcatgttt acctatccag
    24781 ccggaaagaa ctacgaatac caatccatac agaagcgcct gccaaccggc aattttctca
    24841 aacggattgt agatatagcg caagttgatc ttatttttca tacttttcga tggttaagat
    24901 aatttcgttc aacatatttc tgacagttgg atagctgaat ttcaactcgg tagccatatc
    24961 tttcagactt ccgctgtttt tcacaaaact cagaataaat cgctgatctt catccgaaag
    25021 acgggcaaga acaggcaaat catataaacc gtttacttcg gtttcgcatt gttcgcattt
    25081 cagcgacctg actttgaggt gcgcgtggca actcgggcat gaggttggta acatttaatt
    25141 tttcatttac agattatgaa taaaacactc cgcaaatata gcacaaattt caattatttg
    25201 aacattaaat ttaattttat ttattttacg ttcaatttta tttattcgta aataaaatta
    25261 ttcatcaaat tcaatacaaa acaaaaaaag cacctccgaa accggggtgc ttttctttat
    25321 aagaataaga atacctgaat gaattttatt tacctgtaat aattctttta atttcgttca
    25381 gcttattcaa agcctctata ggagtaagat tattcacatc cagccccttg atttcgtcgc
    25441 ggatctgttc gagcaccgga tcgtcaagct gaaaaaagct cagctgatat ccttcgcgct
    25501 gattgccaat ctcacctacc ggtttgctca gatcaccgtt ttgccggttg tcggtttcga
    25561 gctgtttgag tatctgatca gcacgtttca cgatgctctg cggcattccg gccattttag
    25621 ccacatgaat accgaaactg tgttcgcttc ctccacgcac cagtttgcga aggaaaatta
    25681 cttttttatc cacctctttc actgaaacgt tatagttttt tatccggttg aaggtttttt
    25741 ccatttcgtt cagttcgtgg taatgggtgg caaaaagtgt ttttgccttg cagctggcat
    25801 gttcgtgaat gtattcaacg atagcccagg cgatggagat gccatcgtac gtacttgtac
    25861 cgcggccgag ttcatcgaac aacaccagac ttcggtcaga aagattgttc agaatgctgg
    25921 cagcttcgtt catttccacc ataaatgtac tctcgccctg cgagatattg tcgctggcac
    25981 ccactcgggt aaaaatcttg tccaccaccc cgattttggc actttccacg ggtacaaagc
    26041 atccgatctg agccatgagg gtaattaaag cagtctggcg cagcaaagct gacttacccg
    26101 acatattggg accggtaacg atgataattt gctgcgtttt tccgtcgaga aaaacgtcgt
    26161 ttgacacata attctctccg acaggcagct gcttctcaat caccggatgt cgtccctgtc
    26221 gtatttcaat aacatcgcta tcgctgattt cggggcggac gtatttattt tcggcactca
    26281 cccgcgcaaa agaaagcaag caatcaagct gagcaatcag attggaatta agctgaatgg
    26341 ctgcgatata atccgataga cttatgatta actcgttgaa gagacgtgtt tcgatggcta
    26401 aaatcttttc ctcggcaccc agaatttttt cctcgtaatc tttcagttcc tgagtgatgt
    26461 atcgctcggc attcaccagc gtttgcttgc gtatccacgt atccgggact ttttccttgt
    26521 gagcgttgcg tacctcgata aaatagccga acacattgtt gtagcttatt ttcagggaag
    26581 ggataccggt tacctcgctt tcgcgctgct gaatttcaag caggtagttt ttccccgaac
    26641 gggcaaggtg gcggagttca tcaagttcgg cattcactcc ggggcgaagg atatctcctt
    26701 tatttaccag cacaggcgga tcgttctgca attccttctc aattttttcc gaaatcactt
    26761 tgcagggatt gagctgatcg gcaattttcc gtaatgtcac attttcaatg gccagacagg
    26821 cattttttat cggttcgata gcctgtaaag ccactttgag ctgcaccact tcacgtggat
    26881 tgatacgccc tactgccact ttggaaatga tgcgttcaag gtcgccaatc agggcaatct
    26941 gctgttcgag catgtgtttc agttcggtat ctctgaaaaa atattccacc acactgaggc
    27001 gttcttcgat tggtttcaca tctttgagcg gaaaagccac ccagcgtttc agcaagcggc
    27061 ctcccatcgg gctgatggtg cggtcgatga catccaccaa cgtgcggcca ccttcattga
    27121 tggttccgta caattccaga ttacgcaccg taaagcgatc gagccacaca tagcgttcct
    27181 cgtcaatacg tgtgaggtgc gtaatgtgtt cggtctgatg gtgatgagtc atgtccaggt
    27241 aatgcaggat agctccggca gccacaatcc cgtttttcag attttcaact ccgaaacctt
    27301 tcagattttt tgtctgaaaa tgtttcagca gtctttcgtt ggttgtttcg ggcagaaatc
    27361 cccagtcttc cagtgcaaaa gtgaagtatt tggatccaaa gtttgtttca aactcgtttc
    27421 gttttgtacg gtcgtaaata acctctttcg gtgaaaaact gttcagaagc ttgtccacat
    27481 aatcgaaagt gccttcggca agcagaaatt cgcccgtaga aatatcgaga aaagaaacgc
    27541 ctgcctgatt tttattcatg taaacactgg ccaggaaggt attttctttt tgtgtaaggg
    27601 ttgtatcgct gtaggaaacg cccggagtaa ccagttcggt gacgccgcgt ttcacaattt
    27661 ttttggtcag tttgggatct tccagctggt cgcagatggc cacccgcttt ccagcacgta
    27721 ccaacttggg caggtaagta tccagcgcgt gatgtgggaa tccagccatt tccaccgttt
    27781 tggccgaacc gttggcacgg cgcgtcagtg tgatgccgag gatgcccgca gcttccactg
    27841 catcggtcga ataggtctca taaaaatcgc cgcaacgaaa aagcaataca gcatcgggat
    27901 gttttcgttt aatctcgaaa tactgtttca tcatcggggt ttcaacaact tcgttttcca
    27961 tcagtcaatc aaatcttatt tattgtttgc accatcattt tccagtcgta ctttttcttt
    28021 tcttccttca catttttaat aaattcatcc gaacggttat tttcaaaaaa gtcaaccagc
    28081 gcatccgcga ttttattcac ttcaggttct acaacataac caaccttacc gtcgggaact
    28141 atatcggcca aaccaccgac attggttacc aacatgggtt tttcgaaatg gtaggcaatc
    28201 tgtgatacac cgctttgagt tgccgagcga taaggctggg caatcaggtc ggctgcattg
    28261 aagtagaacg ctacttcttt ttcaggaata aaatcagtat gcagaaccac cagatcatcc
    28321 agagcatatt tttttatcat ttcaaagcat ggttccttag gctcataaaa ttcacctgca
    28381 acaagcaatt tgacagggaa ctgccgcaac cgcacatcag caaaggcttg aataagcagg
    28441 tcaagtcctt tgtaacgtcg tataaatccg aaaaataaaa catatcgaaa atcaggatcc
    28501 agattgagcg catccattgc ttccgaacgt ggaagaatag ctccataatg gtcataaacc
    28561 ggatgaggcg ataccagttt cggttttttc tttgtgtcaa atttactgat atcattcacc
    28621 actgagtttg tcatggcaac aaatccgtcc atcgctttca cgaaatattt gggaaacaac
    28681 ttatccagca cagaaggttc gtgaggtatc atattatgaa ccagcgaaat gattttcgtt
    28741 ttctcagttt ttgcaaaccg ggcaatggtt ccgaaacagg gcgccataaa agccatccag
    28801 tagcagaaaa tgattttatc cggattcttg cccttaattt ctttgcctac actgatccag
    28861 ttcaatggat ttacactgtt gattcggcgg gtaattttca agtctgccgg tgcttcgtca
    28921 tccgaaaact gcgtttttcc cggaaaaaga aaagagggat attgcaatgt aaaggtatag
    28981 atttcaacct cattgccttg tctgacaaat tcacgtgcca gacgctcgtt aaatgcagac
    29041 aatcctccgc gatacggata agcagtacct acaataataa tcttcatgtg aaaagaatga
    29101 ttttaatttt aacctgtttt ttgtggtcat aaacatgcaa aaataagaaa taaattattg
    29161 ttagtgaaat taatccgtag agttttcagt aatgaaatga ttattccgtt gaatatactg
    29221 attttacagc agatgaacag gaaatattcg ttgagttaat accataaatt caaacagcgc
    29281 tttttaccga aaactactga actggtttca acttattgca ataagaacgt aacttttccc
    29341 tcttgatgaa aaatatcagc ttttcacagg tacaaaaaaa gcgaacctta cgggctcgct
    29401 ccggattaaa gtattttcta ctttcaaata aaaaagattt ttgaatctgt acggctaatg
    29461 cgtctatcct tcgaaacaat atccgtaacc caggcgcgta acaatctgtt ttccatatgg
    29521 tccgattttc tttctaaggc gggtaatatt gacatcgacc gtccggtcaa gtaccaccac
    29581 ctcgtccgac catattctcg agagcatttc ttcgcgggta aaaacacggt tcatattttc
    29641 caaaagcaat ttcagtatct cgtactcttt tttggtaaat ggtatttcct gaccgtccag
    29701 caataccttt ttattgtcca gttgcatagt aagagtttcg taggtcagcg ttgagggcat
    29761 ttccggctca ctgtttttgt ccacgcgccg caataccact ttcacccggg ctattacctc
    29821 acgaatggag aacggtttgg aaatataatc atcggctccg atattaaagc cggtaagacg
    29881 gtcgttctca gtatctttgg cggtaagaaa aatcaccggg atggatgacg ttttgggatt
    29941 ctcgttcagc atacgagcca tgcggaaacc cgagatttcg cccatcatca catcgaggag
    30001 caagagatga taaacggtca ggtctttctt caaagcttct tcagcagaat aagccacatc
    30061 cacctgataa ccttccgttt ccagattgaa ctgaagaatt tcacagagat cttcttcatc
    30121 gtcgacaacc aaaattctgt acgggttcat tgaataatat aataagtttg tttttgaaat
    30181 aaaatcaaag caggattatt ctctaaaatt gcactgcaaa ttaaccgttt ttatgtttcg
    30241 atcgcgtgta atatgtatta caaatatatg acaaattcgg taaaattgcc tttatccccg
    30301 tttaaattca aaaaaaactc tcagccaata catttacagt atcacggctg agagtttttg
    30361 tatttataag ctattttatt ttgcttcgat ggtaatttta ccatctttca cccctttggc
    30421 agttgcttcg agggtaatgg agccgccctt ttcaagactt tgaaccagag cggtcagttg
    30481 tccgctgaaa agtttcattt tcggagcctg aaaactttcg aggctggcag gatcaccatt
    30541 ggccacggct ttgaagatgc ccgccccttt cacgttgaag ctgatttcgc gttcttcggt
    30601 cgggcagaaa ttcccttcct tatccacaat tttcacatta acgaaggaga tatcttttcc
    30661 gtcggcagta atagaggttc tgtcggcttc cagcacaatc cgatagggtt gtccggcagt
    30721 tttcattact ttttccgcca ccgctttgcc attttcatca taagccacca ctttcactgt
    30781 gcccggctga tattttgtat ccatccacat caggcgatac cgtttctgac ggtcaaagtt
    30841 tttgcgtgct tcgtcagtat cacttccatc gcgtttgatg ctcaggtctt tggtgcgttt
    30901 tccctgactt accccattga taaagagttc ggcagaagga tagttggtat aaacaaatac
    30961 cggggtaacc tgtccttcgc gtcctttcca gttccagtgc gggaggatgt gcagcgtttc
    31021 ctcattcttg ttccagtgac tgcggtagag gtagtaacga tccttgggaa taccagccag
    31081 gtcgacaatt ccaaacaggg agctatgact gggccagttg gtataatacg gtgtcggttc
    31141 gcccagataa tcgaaacctg tccatacaaa ttcaccgata gcgtagggca aatcttcgtg
    31201 ctggatgaaa tcatcttcgg ggaggtttga ccagctgcaa tgttccacat cgtaggatga
    31261 agactgatgg tcgtcgtact gcgccatggc tttacgttca acggggaatt tatacacacc
    31321 gcgtgaactt accgtagatg ctgtttcgct gccgaggata atctgttggg gcaatttctt
    31381 gtaattttcg gtatagaggt gaggcctgta attgaagcca ggaacatcca gcaccgccgc
    31441 aaaattattg ttgatcaccg cattggaatt atccatgccg ttggttacag gacgcgtggg
    31501 gtcttcccgg tggcaaatat cccgcaggaa acggttgcgt ttaggaccgt tggcagcatc
    31561 accctgttcg ggaacttcat tgcccacaca ccacatcacc accgacgggt tgttgcggta
    31621 atgatgcacc aggttaacaa cgtccttttc agcccatttg tcccagaaga ggttgtagcc
    31681 gtttcccacc tttggtattt tccattcatc gaaactttcg gccatgatca tcatacccat
    31741 ttcgtcgcag gcacgaacca gttcaggagc aggcatattg tgcgaagtgc ggatggcatt
    31801 cacacccata tctttcataa tgcgtacctg acgacggatg gctgcatcgt ttacagcagt
    31861 tccaagaggg cccagatcgt ggtgattgca cactccctga aaaacagttt tcgtcccatt
    31921 cagataaaat ccgtcattgg gttttatttc gatggtgcgg ataccgaaag tagtggagta
    31981 ttcgtcaacc agcctgtcgt tggcataaag tttagacatt gccttgtaaa gtgtgggttc
    32041 agttgttgac cacagtctgg ggttctcaac tatcaaatcc tggctgaatt cattttcatc
    32101 gaatttagtc agttgctttt catttttagc aacaataaca ccgaaaggat ttacgatatc
    32161 ggttgacagt cggtagttaa cattcggcgt gttgttgcct tccaccctgg tgcggatatt
    32221 cacctttgca aaatcctgcc ggacaacagg cgtagttaca tacgttcccc ataccgggat
    32281 atgcacatcg tcagtaatga ttacatgcac attgcggtaa agtccggctc ccggatacca
    32341 gcgcgattgt tcgggacgat tttccagacg gacagccagt acgttgggag ttttgtcgcg
    32401 gttgagaaaa tcggttacgt caaaatgaaa agaattataa ccgtaaggcc actctcccac
    32461 tttacggccg ttgataaaaa cctgtgaatt ggccattgcg ccatcgaaca gcacagttac
    32521 gcgtttcccg gcctggtaag caggcagatt accggtattc cgataccagc ccacacccac
    32581 aaaaggcagt cctccggtac gtccggcatg ttccatcgct tctttctgtc catcctgcgt
    32641 gatggctatt ttttgctggt cgttgttaat actgaaaggt ccgttgatag cccaatcatg
    32701 aggaacagtt acggactgcc atttgctgtc gtcataagcg gcattcattt ggtcgggttg
    32761 gtcagtacgg ataaacttcc agttcttttc gagcagatat tccgtgcggt tttgtgccca
    32821 gacaccggct gaaatagcag ctatcagtac ggctgctaac aagttttttc gtttcattga
    32881 taatgatttt atagtttatt gattattatt ctgcgattta caaagttaaa ttatttgggc
    32941 agatatcaga aacggaataa ctttttttga agtattttat aaaacagaca gtaaccgtga
    33001 aagttacagc gcgggattta tctgggcatt ataaagccat tggcatacat ccaaactaca
    33061 tagcgtacaa atttacccaa cagcatggat actgccacaa tccaccagcg acatcgaaaa
    33121 aaacctgacg ctacggcaat gccatcaccg atacctggca gaaaagagaa aaaggcaaac
    33181 cagtcgccat acttatgaag tttagcttcg aaactatcca ttttttcctt ctttatacgc
    33241 aggtattttt ctatccattc cactttaccc agcataccca ggtaataaca ggacatgcca
    33301 cccagccagt ttcccaacgt agcaaccaat acgcattgcc aggcatcaag acctccgaaa
    33361 accagggctg agaaaactac ttctgaactg aaaggcacca ccgttgcagc aagaaatgat
    33421 gcaataaata atcccaggta accccactcg gcaaattcgg ccataaaaca gttttattta
    33481 ttagaaatat aactgataga caacatacgc caaactcaca acacacaaca caataaacac
    33541 aatgtaataa aacaggtttt tgacgagtgt gaaagtatat gcagttagta tagcaaacaa
    33601 tgcagcaatc agcggcagat aatatgtata acctgaaaat ctgaaaatca gcaacatcaa
    33661 caatccgaaa gccatcaccc ttaaaaaaaa gagcatattt ctcgcctgaa taacatcatg
    33721 ccgggtattg cctgataaac cggtcatggc aagactaaaa ataaggaaaa taactccaaa
    33781 gtagatgagc tgaggtatat tatcgtaatt aagaaactga aaatcaaaaa caacaccttt
    33841 catatctgga atcagagtat tctggttcat tacgaaaaac atgatgctga aaacaaaaat
    33901 cagaggtgta ataaatccaa agacagaagc aaaaaaagtg cgaaatgaga aacaccggag
    33961 aagtataaaa ccaatccaga aaaaaggaag cagataaacc agatcgtgaa ctaaaagcga
    34021 accgagagac acacaaaaaa aagccagaaa cgcctgctcg actccccaag tatccttata
    34081 catctgaaga gaaagaaata cagcaactag caaaaagaaa gatgcaatta ccgcaagata
    34141 gttaccatga atctgcagcc aacaagtcga taagagtaaa tatatgaata ctggcatgaa
    34201 tgtacgcgtc ctgataaatg caaacttatt gttcatctga gcaatcagca gcgaattcaa
    34261 tacggtaacg gcaaaggtga tcagatatgc cagaacagat cctgatgaaa gaatattttc
    34321 agcgacaact cctgcaatac caggcatagg attgggatat ctgtacaaaa aacacatgcc
    34381 catccacagg ccaaaactca ccgtataagt gagcgcggta acagcaaatg agggagctat
    34441 aatcttttga agacttaatt tcatacattt agtattctgt tgtgcaaaat tacgcttttt
    34501 ctgcaaagaa taatgacaga tataaaataa aaacaagaga cagaaaatta atctgtctct
    34561 tgttttgtct tttgtcaggt atcttattat ttcagtcccc gttttataag taatgcatcg
    34621 ggattgggat cggcacctcg gaacgtttta taaagtttca tcggttcatc ggaatcacct
    34681 ttttccagga tgtttttgcg gaatttagtt gccacatcct tgttaaagat atcgcctgtt
    34741 tccttgaatg cctgataagc gtcggcatcc agaacagctg cccaggtata actgtagtat
    34801 ccggcagaat aaccgccttc gaaaatatgt ttgaagtacg tactccggta acgcacgatg
    34861 atttcaggta tcatgccgat acgtttcatc gtgttggctt caaactggtt aacatcgata
    34921 gctgccgtat ctttcagcgt atgatagtcc atatcgagca gcgaagcagc taacaattca
    34981 gtattaatga atccctgatt gaatgttccg gctttacgga ttttttccat cagcgcctcc
    35041 ggcatcagtt cacctgtctt ataatgttta gcataggttt tcatcacttc aggctcccag
    35101 caccagtttt ccatgatttg cgagggcagt tccacaaaat cgcgtactac acttgttccc
    35161 gacaatcccg gataggttga ctgcgccagc aatccgtgaa gtgcatgacc aaattcgtgg
    35221 aacatggttt ccacttcgtc cattgtgagc agcgaaggtt tatttgaagt aggtttcgtg
    35281 aagtttccaa catttacaat aatcggccgg tgattaacgc cgtccttgat atattgttcg
    35341 gtaatattgt tcatccatgc gccggcagct tttcctgcac gggggaaata gtcggtataa
    35401 agaataccaa tcaacgatcc gtcggcatcg aggactttga atgtttccac atccttgttg
    35461 taaacaggca tgccggtaag tttttcaaat ttcagtccat aaagacgtgt tgcaagtccg
    35521 aaaacccctt cacgaacatt ttcgagttta aaataaggtt tcagttcttc ttcgttgaga
    35581 tcgaattttt gcttacgaag cttttcggta taataccacc agtcccaggc ttcgagtttt
    35641 tcacccttat tttccgcatc cattaatttc tgcaaatcgg ctgcttcaac tttggctttt
    35701 gccactgcgg gtgtccagat agtttcgagg aatgtataaa cagcttcggg cgttttggcc
    35761 attgtattat cgagaataaa agcagccgga gattcaaatc caagtaactg tgctctttta
    35821 acgcgaagtt tcataatggt gttgattacc tttttgttat cgttggcgtt atcgtggttg
    35881 caacgggtgg tgtaagccat cagcagttct ttgcgcaatt cgcggttttc ggcatattgc
    35941 agtaccggaa taaaacttga tttctgcgta gtgaacaacc actgaccttc tttaccagct
    36001 tcttttgcac tctcggctgc tgcctgaata acgccttccg gcaatccttt caattcatcc
    36061 ttattactta caaacttttt atatgcattg gtttccgcta gcacattctg cgcgaaggta
    36121 agttcggcag aactgagttc tttgtttatt tcgcgtaact gagatttctg agcatcgttg
    36181 agtgctgcac cgctgcgaat gaagttcttg taatatttct ccagcaagcg gttttgttct
    36241 ggggtaagac ccagattagc ccggttatcg tagagttttt tcacacgctg aaaaagtttg
    36301 tcgttcaggt agatattatc gttatgttct gtgagaacgg gtgtaatttc ctgggcgatg
    36361 gagtccagat cgtcgttggt atcggctgac tgtacgttga agaatgcaga tgatactttc
    36421 tttaaaagca ctccgctaaa atccattttt tcgatggtat ttgcgaaggt gggttcctcc
    36481 gtattattgg caatactgtc aatttcagcc tgatgctgtt tgataccttc ggaaaatgcg
    36541 ggcatataat gctccgtttt aattttgtcg aatggcggtg cgccatactc attttcgtag
    36601 cttttgaaaa atggattgtc cgtttttgaa gtacaagcca taactactgt tccaatcatc
    36661 attgcaaaaa atagtttttt catagttata ttatttttta atttattttt atgctgtaaa
    36721 gatacattta ttttttaata caaacactta aacaccttat tatcaataaa ttttttattt
    36781 gtttccctca ataatccata gctattacgg tgtccttatc tttttccaac tgaatgatga
    36841 gttcatccac actgctgaat ttcatttcat cccgaatttt agcaataaac tcaatcttca
    36901 gattccggtc gtacacatct ccctcaaaat ctaaaatatg tacttccaac gatttttcat
    36961 cggaatgacc catggtgggt ctatagccga tattcatcat tccgcggaag gtgtgattat
    37021 cccattttac ccgcactgca tatactcctg tcgccggaat aattttatcc ttatcggatg
    37081 gtgtaagatt agctgtggga aaacctattt tccgtccgac ctgaaagccg cttttcactc
    37141 tgcctgtgag tataaacgga taagtgagca atttattggc ctgtgctatc tctccccgtt
    37201 caagcgcatg cctgacctcc gaactgctga tgtgatgcgc ttcatcggtc gtgtattttt
    37261 ccacctgaat tacattcatc ccaagtgttg ctccaaaagc tcggtattca tcgaaaccct
    37321 gttcacgatt gtgtccgaac cggtgatcgt gacccaccag cagtgtgctg actttcaatg
    37381 tattgtggag tacatccttc agaaagctga aagccgaaag ctgtgataag tctttggtga
    37441 agttcagaac cacacaggca tcgacaccgg tttcggacag aacgcttatt ttttcgtcaa
    37501 gggtggtgag tagtttgggc tgaaaatcgg catgaaggat ttttcgcgga tggttatcga
    37561 aggtgattac caccgagatt ttatgttcat cgcgcgcaat atcccgaacc gactgaagca
    37621 gataccggtg accggcatgt actccgtcga aaaaaccgac agtggcaaca cagttgtccc
    37681 acggaattgt atcattgtat ctgaagatat acataatcaa taaataccgg cattattttt
    37741 ttagaaatca tttaaactca gcgatgtata aggttccacc agccaggtct gcatcccgag
    37801 ttcactggca gtctcgatgt tgtgaatgcc atcatccaga aacaggcact cttctgcctt
    37861 taccccagca tcttcaagca gagcttcgaa aatttcgggt ttcggctttg ccagtttcat
    37921 cttgtatgaa agatagcatt tatcaaagaa atcgttgatg ttttttccgt ttacttcaaa
    37981 cattttttcg gaatagcgga aagataaatt attcgtattg ctcagcatca gaattttgta
    38041 ttttttcttt agttcagaca gtaaattcag tttttcttcc ggaattccca ccagcaatga
    38101 agtccatgcg tgatcaattt gatggtctgc aacttctccc gaagcgtttt ttctgatttc
    38161 atccctgaat tgcgcgtcag taatcattcc catttcgtaa tcgagcacaa atcccttctg
    38221 aagcgtggtg ctcaccagat tcttcatatt gaaaattccc agattttcga aattatgaat
    38281 acacaggttc cagtccagat caataataac acccccaaga tcaaatatta ttgttgttac
    38341 atttttgtat tttgacataa ttcatcgtta tttttttaca caattcattc atttcagagc
    38401 aagtacatca ttactccctg atttaacaca gcactgctga agttttaaaa acattcaaag
    38461 atactacatt ttttgttcgc gttattttgt ctggtatttt aaaaagaaaa agacctgaca
    38521 attttgttga aaaattatgt ttgtcattct gcgaaacgaa ccgattctga gacagcaact
    38581 tagttacttt tccccgccat atcactgtat atcattgctt ttaatgatcc aatccgattt
    38641 ttacatcacg cataaagtag ttgtccggtt acaaattcct gtccgatgaa ggaaagaatt
    38701 ctcatttatc tctgtgagca tttatagtat tttagtttat ctttgcaaaa aaacttagcg
    38761 caaattccat gttatgataa aaaagattaa ttcgaggcta tgtttccttt tggtaattat
    38821 tggatttatt tgtggatcga tacaagcaca agggttgaag ttttacggga tcaaagcatc
    38881 catagaagaa cgtacttcat tctttgtttt tgaagaaggc tcctctcctg tcttctcaca
    38941 atatattgac ttggacttcg aattaaaaat acaagagttt ggcagttttg gctatttact
    39001 tcatatagtt gataaccaaa acaataccgc atacagtttt acatatacta atatcgacaa
    39061 attatcaagt gcttttaaat ttaacattga aggaaaagcc aatttaattt cgatgaacgt
    39121 tgtcaacgaa tcgattaaat caaaatggct tcccgttcga cttcacatcg acttaaccac
    39181 cggacagagc tctctatcaa tcaataacat aacaaacaac agcgagaaaa aattaaatat
    39241 atcagaaaag tttcagccac ttctggtatt tggaagaaga gaaaacctgg tggacgtaac
    39301 tccgtttgcc attcggaatt tgaaaatatc gggagcaaat aactcgttcc ggtttttgtt
    39361 gaatgagagt accggtgaag cagtacatga taccaacgga aaagttcgcg gcagggtaat
    39421 tcatccttac tggctgatca atgagtcttt tcattggaaa aaaacggtta ccatcccatc
    39481 gaaatcgatt atgggttcga aattcaatga aaaggagcaa tgcatctttt ttataaatca
    39541 ggattcgcta ttcagattca ggtcaggttc aaacctgttg tcgggcgaac catacgcaaa
    39601 taagctccct gttaaactct tattaggaac aaattatata gatgaatcgt ccaataaaat
    39661 atgcgcttac gaaataaata accttccagc cggtgacatc acaatggcgg ttttagatat
    39721 tccgacacgc acctggaaag ccgcagggag agcctttact cctgttcagt tacatcatca
    39781 caacggattg ctgaacaaag acggtcagta tctggttttt ggtggtttcg gcaacaaacg
    39841 ttacagcaat aatttcctgg cttataatgc agcaacagat cgctgggata cattgacttt
    39901 taaaggcgac agaattacac cccggttttt ctcctcaata gcttcctcca aaaaaggaga
    39961 cgttctgtac atttacggag gagtgggaaa tgatagcggc gatcagactg ttggacataa
    40021 ttactacaat gacctttatc gggtgaattt agtaaaccac acgataaaaa aatgctgggg
    40081 taatccatcg aacacaaagt tagtttcgag tgagcgtatg attctatcgg acgatgaaaa
    40141 atacctgtat gtaatccgat accccgagta caatgaaata actcatctgc aacttcaccg
    40201 aatttctgtt gccgacggaa cttcgcagtt attaggcgac tcgataccgt atatttcaaa
    40261 atcaatagca tcaaatgttt cactttacta taacgctgcc ttacaggagt tttactgtgt
    40321 tactcaggag tttgatgatt ttgcccatag tgtaaatgca cacgtattta ccttatcggc
    40381 tccacctgtt gataaaacag ctattcagtt ttattcagct cctaaatcta attcgttatt
    40441 tataattatg attattttag tacttgtact aagtgccggt tttatacttt tcttttactt
    40501 gagaaaaaag caaagaaaaa ataagataga attaagtaca gttcaagtac ccgttcaaat
    40561 aaaatctact atagcatctg cacataccaa aaccccggat ataacagatt taccaataaa
    40621 agaaacagga tcaacaactg agtttaacaa aatatacatc tatggcattt ttacggtgta
    40681 tggtagaacg ggaagagaca tttcttatat gttcagcaac aagttaaagc tgatttttct
    40741 atacattctt ctgaacagcg attcggaggg tgtgagctcc tctttactga atagtctttt
    40801 ctggccggaa aagatggaaa agaaagcaaa aaatctgaag ggcgtaacaa tcagtaatct
    40861 acgaaaagcg ctggcagaaa ttgacggagt ggaacttatt tacgataaag ggtttttcag
    40921 aattattacc acagcgcctt gctattgcga ctattgccac ctaaaggttt tgcttgactt
    40981 aaattctcat gacagcgaag aaatgctacg cattctggaa cgcggtcaat tgctggaatg
    41041 caccaaacag gaattcttcg acaagtataa acaacaaacc gaggatctta ttttttctgt
    41101 tttacctcag gaattgccgg ttctttacaa gaaaaatgaa cataaacagg tactgcgaat
    41161 ttgttctgtt atgttaaagc tggatccttt gtatgaacct gcactggcgt atagcgttta
    41221 ttcgcactgc cgcctcaatg agtacgagaa actattcaaa acatacgcat tatttgcagc
    41281 agagtaccgc aactcgatgg gacagactta tccgaaaagc ctggagaccc tgatacagga
    41341 agcacaataa cccatatttg attttcaact cattgaaata cggaaataga ccgaatcaaa
    41401 acatctgatt cggtcaacta ttttaatatt cattgcctat ctcaatatga ggcgtcattt
    41461 taataatgtt actcttaatt tatgaataat agcatcgttt acctcccgat tcatattttc
    41521 attttcaggt ctgtattgta gctgaacctg atgctcaccg gcttttagat ttacatgagt
    41581 agaattactc catcctaagt tttgccactc gttacgccct ctttgaggaa aaatggaaac
    41641 gccctcatac attccgtcga ctgataggga ccggatacaa catttgttct cgtcctgtac
    41701 cgttgcacct ccgttggcat ataaccaatc gatagcatag aaaccgtctt tcgccacttt
    41761 aattgttgta ctaaaaatag tactgacttt ttcgcctgat ttggttatgt tagccgattt
    41821 ttcgattctg gtttcgtcaa tattgtagat agtttcgctc tgtttccgcc aaacaagagg
    41881 ttcactggca aaagatgata atattgggtc attgcatgta gcaataatct gatattcacc
    41941 ttcgtcggtc gatttcagct tgagtgatgt gtttttcagt tttttgtatt ctctcccatc
    42001 tttaagtata gtgtatgata atgctccatt tattggactc caggataaaa ttccatcgtt
    42061 aattttagcc actggattca acaaagtatg tttgttatcg gtaagattta taacactgtc
    42121 agagtcgaca aaactatttg tgagtataat tttcacatca tgttctccac tcattgtgga
    42181 tagcacttcg ggagtgcctg acttaccgtc gatggtaaac aaagctatcc ggtctccaaa
    42241 accttccata ctgatgttta gtttcgcttt tcgataagag aagtttgtaa gattcctttt
    42301 tccggctaat tctttgggta caaaagggtt aaatatcagc ttatcttctt caaatttcat
    42361 cccgaaataa agacggtaaa cgatgcttaa attacccgaa aggctccaaa gcatattgct
    42421 tgagttagta tgagttccag tccagtcgcc gctttggaca tcaaaatttt ccttgttagt
    42481 ggcaaacaaa gctgtggctc tgtatgtact tcccaatgca tgttcaacag ctttttgatt
    42541 gccaacttta gccgctgcca acatccaata cgaagttaca aatggccatg tagcattgtt
    42601 gtgataagag aacatatcgg aaatttgagg ataaaagata cttgggccaa aagggagaac
    42661 cggcatgttt tgaataattt gcttctgttg ttcgggcgat gctatatcga acagaataca
    42721 aagagcctcg cctaatgttt cgctgcgtgg agataaaacg ttgcaattgc gtccatacat
    42781 atattgagcg taatatcctc tgtcaggcat ccacaaatgc ttattaatag cagcttttaa
    42841 agactcagct ttttgactat atctcatgta atcgtcttgt ttattcaacg tttttgccat
    42901 tttgcttact atatttaaag cctgataatg gatggcattc gttcctaaac acttcgattg
    42961 aaatatgtcg gctggttgca tccatttcgg atagctttgt tcgcgccaat cgataaacga
    43021 cgactcgccc atagcaagtc cggtttcttt atcataaact gtttgcagat cggtttccac
    43081 ggatttcttt atggcaggat atacactttt catccaatca aagtctcccg ttactttata
    43141 caactcccat gcagccgtaa tccacacaac acgatcggtg gaaacaggcc acgaacctcc
    43201 ggtgccggtg tcttgtatta ttctaccaac catatctact ttgcgtaaaa gactattttt
    43261 tgatacagtt ggttgcatgt aggacatgct caaaaagata ctgtagctta catcgcgagt
    43321 ccaaacacct ggccaaagta ttccggtacg caaagtcaaa tccggttcta cagcattcac
    43381 catttcgtcg agtcccaaat tgtaaatcgc attttccagc aaggtaggac cggaatattg
    43441 aggataagaa gaaatatcgt ttttcttagt ccagacatag ctgatcagat cgtctttgga
    43501 acttggactg gaaaaattac tgtggatttt tgtagttgat tcagctgtac ctacgtagtt
    43561 tccttgtatg attttgtctg attgccaacg atatgcatcg ttttcataca gaacctgagt
    43621 ttttgcctga tttgcgatac ataaatacaa agtcaatatt aagacgaatt tgggtgattt
    43681 cattctctct tatttagttg attcttgttg caaagataca attatacaat tgattcaaag
    43741 gttctattct ttttttttca ccgtaattgg atcaataaaa gcaataatag agtttctttt
    43801 attgtaacat gtcatttcac atcattacat gatttgtcaa atttatcgtt tgctgctata
    43861 ctttaattct actgtttatc tgacacgacc agcatttctt tctctgccga ataaatcaaa
    43921 tgtcccaaac tacttacacc ttccaaaata atccgataag ttgttggaat atctgccgca
    43981 taaaacgaca aattgaccgt tcctgtacta tctacctgca cattaggatt ccaaagtatg
    44041 gtggtccggt aatcgggtgt actactgttt ttttcctgtt ccgtgctata aacaggagaa
    44101 taaaacttca ccgtttgttg ataaccaagt gacttgacaa cttttatatt aggatttatt
    44161 gaattctttt gtacaaatcc atttttagta ttaataacaa tagctccatt agatcccatt
    44221 gttccaaaaa tggctcccat cgttgaacct tgatttataa aaatatcctg gatatcatct
    44281 atcatcagca tactgtaatc aaaatcctcg gtgggaacat tgtccagcaa caacatcggt
    44341 ttattgcccc ggtacctaac atcactaccg ttaaccgtaa cgccggctaa tctgcgcagc
    44401 aattcatagg tagagcttac atgccaaccc tcaatgtctt tagcagtaat caccctggat
    44461 gaagtaaccg aataataggg cgactcggtg acagccgcaa cttttcgcct ggcagttacc
    44521 attacctcgc ttaaatgata ggttttcatc ccatcaaatg ccaaatgttt cttgttcgaa
    44581 ggcaataata tagtatcttt caatattgac tctttgcggt tgattatttg agaagcagcc
    44641 aacgaaggga aaccagcaat gctatccatt tctaaaaaag cctttttaaa tttgctattg
    44701 gaggtagcct gaataatata ccttgttcca tcgcaatatt ccagattttt aaaaacgaac
    44761 cttccttcgt tatccgtttt agcaagcaac ataccttcca cagagtcttt taatgcaagt
    44821 aaactgatat cgccctcttt cagcgatgaa aaaacgcttt ctgctttccc cgaaatttct
    44881 tcgcttaatt ctaccggata ttgtaaaggc ttcgttagtt tacccttcag aaggttggga
    44941 atatcgtatc ttcgccatcc ttgcgtcatc attaaaacat ccaatgcagc ctccgttttc
    45001 ttactgtctt ggcggaggta gcttgccggg tcttctatat atccctttat ttctgatgta
    45061 agcaacaagg ccgaaataat gtttgaagcg ctgtctatat ccgctccatt tgcatccact
    45121 actgccaatg agaaatttcc cgacaacggc gctttgtttt catccgtaag gtgtaccgac
    45181 aagtttattt tttctcgcgt ttggtacttt tctttattta attcgatatt agtttggacc
    45241 agagcattgc cttgcgaaga aaacacaaga cgttcactca gaatattccg gtctttgtca
    45301 accaataaaa aatgaacaat tccggcaggg aagaaatctt tttcgaaacc aatacaactt
    45361 ttattttcgt cccacggctc cacatagatg ggtactcccc ggatatgaac tattaattgc
    45421 atttgtggcg gcaatataaa atcaggtgat ttcaatactc taacaagcag tttatcgtcc
    45481 tcccagactg ttttcaaaga gacagcactg tccgaaggtt cgggcaaatc aaaccgcttt
    45541 acaacctttt ctttgttggt acatagggca taatactttc tgccgggctc ataggaaaac
    45601 ttgaaactgc ccatccccag atgtgtactt tcaaactcgg cacacaaata atgttgatca
    45661 tcaaaaacct gccctttaac ctcctccgat tgcccttgcg tattgagggc tttaaatgcg
    45721 attgttgtat tagccgacga gggagcatat cctccttcgg ggaaaaaaga aacatcgaat
    45781 gtattttcta tatcgggaat tttaaaatac cgtttataca ttctgttttc gatcactgcc
    45841 tgtagcaaaa acactctttc tttgcttact tctttccctt taaaagagca tgatcctgtt
    45901 ttctctttaa atgtcactgt tttagcatcc cctttctcag taccccccaa ataaacaacg
    45961 cactgcccgg gcataagttt ttcctttctt cctttagcag aaaaaagcag ttccgcatta
    46021 actccatcac cttctgcctt atagttaata tcaacagaca ctttttctga taccggattg
    46081 gaaatataaa gctgtttgga gaaaaaataa tcttcccctg catttctcat aaatcgtgta
    46141 taagccctta ataaatagtc cccctccccg atctcctcat ccaactgtat ttgtccgtaa
    46201 aaacaacccg tactgtcagg ccgcaatttc actctttgca caagatgttt tgccggattt
    46261 atcagttcaa catatacata tcgacttgcg ttggcttgtt tcataaaaat ggcatccacc
    46321 aaatgtaccc gcatccagat ggtttcgccg ggataataac cgcctttatc ggtttgtaca
    46381 tagatttttt cttgcgggta agcagacagc tgatcaataa aaagcttttt aatggggttt
    46441 tcttcagact cttgcgcata aagaattgtg tcaaaagcaa gtagtactaa taaggttagg
    46501 atcgattttg ttttcatgta ttattggtct tatcttttat agctaacacg ttcctttagc
    46561 tcggacttca ccggtttttg gaacgaagca tggaaaacaa taatttccgc cacaaataat
    46621 agtgttgcaa ctatgattat agatgtgaca atctttaaga ttcttttctt catattgatc
    46681 tcgtcattaa aaaatttatt ttaaaacaat aaactaagga ataggagtag cagaaggcgg
    46741 tgcatcttct aaattgcttc cccagttttt atttgcttta tttcccataa ttaatatcag
    46801 cgatccgccg ttggcgatat ccgaatgatt aaaccatggt ttattccatt ccttgccatt
    46861 taacagagcc gattggatat acttattttc ttcggagcaa tttttggcaa caatttcaaa
    46921 tatctttcca ttgcctaatc gcaccttcac gtcgttaaat aaggggctgc cgatattata
    46981 cgacatcgaa cccggagtaa ccggataaaa tcccatggaa gaaaatacaa caaaggctgt
    47041 cattccgccg ccatcttcat cgccgggtac tcccatcaaa tcgttacggt accaataacg
    47101 aagcagttga cgaattcgct tttgagtttt ccagggctga ccagcataat tgtataaata
    47161 cggaatatga aacgacggtt cattggccat agagaactga cctacattgc cagtatgatc
    47221 gggcaaagag gaatagaaat cgtatttgct ttttcctaat ggttctctga aggtagcatc
    47281 caaatttcgg gcaaatttgt cagctccgcc cattagcttt actagatcag caacattgtg
    47341 ttgcacatcc caacgaaagg tccaaccgtt attttcgtcg tagtattctc gggctcccgg
    47401 tccaccagaa aaccgataat cgaacggttc aataaacttt ccttcagcat ctttcggatg
    47461 aaaaaaagaa gtctccgggt tgaacaaatt ccgataattg taagagcgtt tcaagaaata
    47521 ctgataatcg tcggttttat ttaattgttt ggctatctgc gataaacacc aatcgtcgta
    47581 acaagtaccc aaagttaccg ccaccggttg acgtttttcc caggaatgaa cctgagcaca
    47641 attttccttt tcgccgggct ttagcgccgg gaaatatcca ttggaattat aaaactgatc
    47701 aagttctgtg accggcactt tcatccaggg gagtaacgac ttttcggtca atgctccttt
    47761 acacgctttg taagcagctt taagatcgaa accacgcaat ccttttgaat aagcatccaa
    47821 aatgaccgcc acagcatgat tcccattcat ccggtgacta tcgccggtca cctcaggaaa
    47881 agtcggcatc cagccttgtg gcgattgttg agccatttga atgtaagagt tcaccatatt
    47941 agcttccatt tccggctcaa tcaatacccg aagcggatga gtagcccggt acgtgtccca
    48001 aatccagtcg tcagtgaaaa aagcgtgtcc accgtcatcg tgtattttgc cgtcgaaagc
    48061 actgaagtat tttccctctt cactgatatt gatcattcgt tcgtacgttc gatacaaaga
    48121 ggtgtaaaaa acagttttct gatcttcggt gcctccctca attgagattt tactcagggt
    48181 cgcattccaa acagcacgcc cggccaacgc aactttttct acatcgtaaa cagtgatttc
    48241 tttgttcaaa ttcttcttgg cttgctcggt gctaataaac gaaataccgt accgtaaccg
    48301 gatatttttg tcatttttat taaatcgcag cactaaagct ccgttattcc cactttgttt
    48361 tacctgatag ttgatttggt cttttcccca gcgtccaaca cttgttggaa tttgttctgt
    48421 ttcaacatac aggtaaattt tagaaatact attatcaatc acctgaaaac cggaaatact
    48481 tttaccgtca gtctccaaat ttccgtttcc cacatttact acaatgtagt tatcgtcttc
    48541 tttatcaaag ttgaactcat aaattcccga atgataagac ggtgagtagt ttacatgaat
    48601 gttagcctcg tccaaaaaca cagaatagct gtagggagtt atcttttcca aatcgtatga
    48661 ataagcctga acacgagaat tggatactgc tttgccctga atggggctga tgttaaaagc
    48721 agaactacca cgatgactgg ttacagccac aggcaaccca tgtattacat ccgaagtata
    48781 atcagctcgt tccggataaa cgcgtaacat actgttcggt aaatgcacag ttgggtaagt
    48841 tggcaccagt aaatgactaa tgtttccaat taacggattc acataatcta ccggtaattt
    48901 cacattgctt cccatacatg agcaataagc cataatgccc aacaacaata aattcttctt
    48961 taatctcatg gcaattcttc tttttttatt ggtatttgat tcttatttta ttcaatgaca
    49021 aattgtggag gtaaccatct tattgaaaat tacacctgat ttactacaat aactatcaac
    49081 atgaagtata ttagtttttt tcctttttca aagccgcatt tcgtttctcc aattcgtctt
    49141 ttactttcat agccatttct gtgacatttt tcctgatttc cggcataaat ttttgcatca
    49201 caatcgtcac tatttctcct tgtatttcag gcgccttttc aatcactttt ttaccagttg
    49261 aggatttata aaaggtaacc aggtctagta tttcactttc ggtataatac ttttcataaa
    49321 ttccggcaaa atcttcgtcc ataaagaaag aaaagtttgt tcatatttta attcaaaatg
    49381 tcggcgcatt caacattgga atggcatcgg ggtaagcaaa ttcatgcagt aaagtcccgt
    49441 cctttaatcg gacccatgaa ctgaacttac gttgtccttg ttgcagcaca atcactctgg
    49501 ctccgttttc gtaatgtgtg tacgtgttat tgctaccgga aaaccggcca taccccaggt
    49561 aaattccgaa tttattgaaa acaaaatcgt tgtcatgatc gtgtccggca aagcacccca
    49621 tcacatcacc accttttacc atcgcagcaa acatgcctgt gttaatgacc ggaggacatg
    49681 gtttttcttt atacatgccg atatagttgg ttgatttatc gctaaggtcg gcgtattcaa
    49741 gcaatggaat gtgaaaaaat gccagagaag gaacaggctg tcggtgatta ttattggtct
    49801 gttttttact catcttcctg taccattcaa cctgaccggg actaaaacct gcatacactc
    49861 ttttgccagc aatggagtca taatcgttgc tatccatgca gtataacaat gcttcagttt
    49921 ttgtagtacc ggcatttttt atctctataa gatagttgtt ttcgcctcta atctgtttaa
    49981 caggtctgat attgtagtaa ccgggaagtt cacgtgtcat tttacccaac atttccctgc
    50041 tgattttcat tccaggttcg gtatcatgat ttccaaaaag aataatgtag ggaatatgac
    50101 gcgacacaac gggttcaaca agcagctgca atccttcttt taaaggcaaa tccaccacat
    50161 tatcgccggt aaaaatcaca agatcaggct tttcaaaatc cagtacggaa cgcattgttt
    50221 cgatggtttt catggaatta tcgagccttg aataacagat atgagtatcg gtaaactgaa
    50281 caattttgaa tgttccatcc ggattaaact ttaatgactt tggcttagca gcaatgctcc
    50341 attgagtaaa gacactgaaa gaaagaagaa tgaagagaat acttaatttt ttcatgatta
    50401 catattaata agatctcata acctcacttt atagagaaaa aaatgagtaa acgatatatg
    50461 agataaaagt attcaataga aacgtaaaat acaaggctgt atcaaaagag ttttgtaaat
    50521 accactttca atacagcctc tttcccaatc taaattaaaa atcacatcaa aaaaaactag
    50581 cgaacttttt cagaatttat caaagaatct tacttagaac tttccggaca acctgttcca
    50641 tcatcttgta tccttccaga ttgggatgca cttcatcccg ctggtactta acgttcaagc
    50701 cttgcctgtc atcaaccata gccgaataat aatcagcgaa cgcaatttta ttttcaaggg
    50761 catagctttt aatcttttcg tttaattccc ggatctgatt gatagattca acttcgcccc
    50821 gccagggaaa atgatgagca ggtaacacag agcacaaaac taccctgatt ttatgctgtt
    50881 tagccaattc ggccatggat gcaatgtttt cgaaaatatg ctgtactgaa atatatccat
    50941 tgttcagtgc aatatcgttt actccggcta aaatcaccac tgctttgggt ttcaactgaa
    51001 taacatctgc ctgaaaacga accagcattt gagaagtaac ctgtccgctg atacctctgc
    51061 caatataatt gttatcggta aagaaatcag gatcggcgcc tacccaacct tcggtaatgg
    51121 aatttcccat aaaaacagcc ctgatcggtt gttttaccgt tttatttgcc tcagcatatc
    51181 tgccaaagtt ggcccaatcg tgattacctt gggcttgaac agaagtactg cccaaaagca
    51241 cagaaagaaa aaagattgtt tttaaaagtt tcatatttat tcaaaattaa ttgatagtta
    51301 acaacatttc tacattaatt caacaacgac agctacattt cctcttgccg atgaactcac
    51361 aaaagggttt gcttgctgtt tcccatttat tctaaaagat ttgatacgtg aaccttttcc
    51421 ttttatggta atatccaaac ttatgttgcg gtaacgtatc cccgaaagtt ctgctacctg
    51481 aatgccatcg ggcagatgcg gctgaaacag aatgccgtca ggcatcaacc gcaagcctag
    51541 caatccggaa tgaaccatat taataaaagc cgtagccgac caagtctgca agcggcacga
    51601 tacccaatga tgctccggat tgttctcgcc aaactggtat ccgccatccg gttttccggt
    51661 atagggatta taaatctcaa agaagctgta attgccctta tcttcgtcca atgctaatga
    51721 aataagactg ttcagttcat gatcgtaggt tttataactt ccaacaaata ctgccgcatt
    51781 agaaaagaag ccgttcacca taggccaaat tatattgttg tgcctaccag gcatttgagg
    51841 cgaaaacctt ttataatctg gataaataga ggtaatgcca tagtcagaca ctacagtatt
    51901 ggcaatagca cggagtgcct gttccttatc caccacaccg aaaactaccg caaacgaaag
    51961 tcctaatcct tcctgcgaat gatcaacacc tccattctga tcaatcaggt agttgaacgt
    52021 tcctctttcg ggattataca ggtatttcaa aatattcgac ttcaaggctt ctgccttttc
    52081 tactatcggg gttgaaagac ttgcctccat ccgaagttgt ttctgcattt cgatgagcgt
    52141 tttatagaca ccgtagtaca cgcaattcgt actcagacat tttaccgtaa atggtttcag
    52201 atggtcaaca gtagcactgg aatgaacctg aggattgaag ataggctcat cgtaagcagc
    52261 aataccatcg ttgaaaaccg atggtcccat aaaaagtccg tatttcgggt caaaaacggt
    52321 agtttcaagc tcttttatgg tagtaaccga acataaataa gcttgtcgga gaaaatcctt
    52381 atctccagtt accagataat gattgtaagc tgctattacc caaataattt tatcccaata
    52441 ctgatgacct atactttctt tatttatagt aacgctccac aaagatcgtt gagcaacttg
    52501 aggacgtaac aaactagccc caaaccatga attgatggca atgtcacgtg tccattcacc
    52561 tccataatca ccaccagcag ccaatattcc ccgacgggta ttgatatcaa gcgtatttac
    52621 agccaaacga taggcatcgt tcaaacgttc gttgtcagaa cgcaacgttt gcgcttgcaa
    52681 gaaaaccgta aacataagag caaagactaa aatgatattc ttcatattaa aatatctata
    52741 aattcattat taaaaggtag aaccccagaa caggctctgg attcagattc agccgcaagc
    52801 gtggagaatt caaccttagc tattaaactt aagcatttgt t
//
LOCUS       DAOPYB010000003        50014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_545_length_50014_cov_9.513601, whole genome shotgun sequence.
ACCESSION   DAOPYB010000003 DAOPYB010000000
VERSION     DAOPYB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 50014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50014
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_545_length_50014_cov_9.513601"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /gene="cas2"
                     /locus_tag="PKH58_00440"
     CDS             complement(<1..178)
                     /gene="cas2"
                     /locus_tag="PKH58_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009231974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNX87521.1"
                     /translation="MLSKLNQYRIMWVLVFFDLPVVEIKERKVAARFRKDIMADGFAM
                     FQFSIYVRHCASAEN"
     gene            complement(212..1147)
                     /gene="cas1"
                     /locus_tag="PKH58_00445"
     CDS             complement(212..1147)
                     /gene="cas1"
                     /locus_tag="PKH58_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HNX87522.1"
                     /translation="MIKRTLYFGNPAYLHLDNKQLIVKLPQVEKNAEMPEMLKKEAIA
                     SIPVEDIGVVILDDKQVTITQALISTLLDNNVALISCSENHMPSGLFLPLNVNSMQNE
                     RFRAQIDASVPLKKQLWQQTIISKIRNQAAMLKKYGKDASWLMKWAGDVRSGDPDNYE
                     AQAAAYYWQHIFPEKLGFKRFREGEPPNNLLNYGYAILRAVVARGLVATGLMPTLGIH
                     HENKYNAYCLADDIMEPYRPMVDRVVCEIVYSEIDFTELNKDIKQKLLSIPAMDISID
                     GENSPLMVGLQRTTASLFKCFEGSSKKIIYPELNF"
     gene            complement(1313..2011)
                     /locus_tag="PKH58_00450"
     CDS             complement(1313..2011)
                     /locus_tag="PKH58_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87523.1"
                     /translation="MHFEPEHSYHIFNQGNNKELIFFNRENYFFFLKKIETHIVPFAD
                     VLAYCLMPNHFHLMVYTSHQMTLSHQMTSQIATDQATLSHQMTSPQIATDQMTLSHQM
                     TSQPSHQMSEGKQTLVQSIAIMLRSYTRAINIQESRSGSLFKPHTKAACLSNNSTITP
                     AFSDTIHGAYINIPYPDKEYPKLCFNYIHNNPVKAGLVKDANEWEFSSYCDYYENRHN
                     SFINFLRAKECGLI"
     gene            complement(2098..2568)
                     /locus_tag="PKH58_00455"
     CDS             complement(2098..2568)
                     /locus_tag="PKH58_00455"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNX87524.1"
                     /translation="MNITHPAHQEMLAALLENKVDFILVGGYAVIYHGYIRTTGDMDV
                     WLRPSENNKTKLIKVFTQLKFNPDDISLIEKLDFSGTVIFHIGAEPDRIDFLTRVDGL
                     NFEEAFNNRVFLKIDDNEIPILKLEDLITNKIITGRLKDKADIEQLNLIRERKK"
     gene            complement(2565..6542)
                     /gene="cas9"
                     /locus_tag="PKH58_00460"
     CDS             complement(2565..6542)
                     /gene="cas9"
                     /locus_tag="PKH58_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HNX87525.1"
                     /translation="MKKILGLDLGSASIGWAVINENEENSKTACEILGLGCRIIPYEG
                     TEGKDFAKGTGESRNSLRTQARTARKGYDRYQLRRKYLVDILIKNNMMPDDALKALPK
                     KQLWALRDKAAASEISPKELGRILLWLNQKRGYKSSRSDANLDKKDTEYVATVKSRHD
                     KIKELNLTIGQYFYNELTKDEYFRIKENIFPREANIEEFDTICKHQPYLSQELIDKIR
                     NEVIYYQRPLKSQKGLVSVCDFEGFWTKKEGKEYFVGPNVAPKSSPLFQLAKMWENIN
                     NIKINSRTGGDVVLTSEQKWALFEHLDNNEKLTQTDLLKILNFKKENCYVNKQLAKGL
                     QGNVTKFAIKKCFASPNEYDNLLKLDLNIIEGKGEGYLYDKKTGEILDAKPIKFIDPL
                     VEKEPFYQLWHTIYSINDQAECSTVLQKKFNIDKITADKLAGIDFAKFAFGNKSAKAI
                     RKILPYLMEGAGYSEAMSYAGYDHSNSLTKEQNLERKLLDQLKPIAKNSLRQPIVEKI
                     LNQMVNVVNAAIEQYGKPDEIRVELARELKQSKEERNDTDKAMNERQRQNEKYAKSLA
                     EFGLRATRNNIIKWRLYEEIDKQDKKMNAMCIYCGQPISLTEAIKGNDVDIEHIIPKS
                     KLFDDSQSNKTLAHRHCNKNKNDQTAYDFMKGKSEQAFHEYVERVNMLYDNHIIGKAK
                     RDKLLMTESKIPKNFIDRQLRESQYIATKAREILQSICHNVWSTSGNVTAELRHLWGW
                     DDVTMNLQMPKYKDLGLTETKEWESEHGQNKHTKEVIKDWTKRDDHRHHAVDALTIAC
                     TRQGFIQRFNSLSSSKTREDMYAEVEKCSDLFNEKRSLLEKYIISQKPIEVKDVENAV
                     SGILVSFKAGKKVAVKGTRKTGKKGNKKVVQTGIIVPRGSLSEESVYGKIKSIEEKKP
                     VKYLFENPHLIFKPYIKELVENRLLHYDGDTKKAIASLKKEPIYLDKEKKILLEYASC
                     LKEEFVIKYTVNTDFNKVDKVIDLKVKEILQNRLNKFNNKPKEAFKDVQHEGKTLLKW
                     YEDEGLERPIKSVRCFTGLSAVVPVKKDENGKNIGFVKPGNNHHIAIYKDSEGNHVEH
                     VCTFWHAVERKKYGIPVIIHDANQVWDKIAEAPEDTYPESFLVKLPLANWKLNLSMQQ
                     NEMFVLGMSQEEITLALESKDYMAISGKLYRVQKIFCNPLAKQIEIVFRHHLETQLID
                     DSNAKASRRFIKVQSLGALFSLQPFKIKIDCLGNISLTNPLNKSNRNSYDSDNTAKEP
                     VNSYATTQKITIFQSPEDAKNSQLDYWAKLTPEERFERFLELMNRFYKFVKPDWSTKK
                     IILDL"
     gene            complement(6806..10924)
                     /locus_tag="PKH58_00465"
     CDS             complement(6806..10924)
                     /locus_tag="PKH58_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX87526.1"
                     /translation="MKMKNFFSLLWTILIIHISMGTQAQNPTYLCELRNDTQVSSTVF
                     EFDIYLLQTGSIPFEYAAGQYGILINPLIKNGGVISASIVAGSSDPALIAASQNPTSI
                     NFLAASNCIRIAGRVPPGAGNGAVISNGSPGTKVCRVRLTNTVAFGQFSPNLTWTTTN
                     IYPTQINAYVGGVNTAIMVPASQTTNNLNNLVLNQNLPIVYAVTGSGSYCFGAGGLAV
                     GLANSEIGVTYTLYKDNIAQIPTLDGIGAALDFGLQVAGTYTVVGTNGSGSSAMSGSA
                     VITEIPIPVAPTVNVIDNCGSSTLTASAFTGTLLWNTAETTASINVSSTGSYTVTQTI
                     SGCTSPAGSGLAAPLTIPATPVVGTITQPTCTAATGSVILSGLPAGNWTINPGNITGT
                     GTSTTISSLATGTYNFSVNDGTCTSIASANVVINTQPGTPAAPTLGTITQPSCTLATG
                     SVILTGLPAGNWTIDPGAITGSTTSTTISGLIAGTYSYTVTNSAGCISVASANVVINA
                     QPLIPTAPVVETITQPTCVLATGSVLLSGLPAGNWTLNPGNIAGSGTTTTISGLAAGT
                     YTYTVNNGTCISSASANVVINAQPLTPGAPTIGTFTQPTCLLATGSVVLNNLPAGNWT
                     INPGNITGTGTSTTVSGLAPGTYNFTVTNSAGCTSAVSANVVINAQPITPAAPTVATI
                     TQPNCDLATGSVLLSGLPSGNWVINPGTITGNTASTTITGLAPGTYNFMVTNSSGCTS
                     VTSTNVVINVQPVIPSAPVVGTITQPTCTLSTGSVLLNGLPAGNWTINPGNISGTGTS
                     TTISGLAAGTYNFTVNDGTCTSVASANVVINTQPETPVIADQSTSISSGATFTVIPAG
                     VPAGTTYTWTPPTYTGGVTGGSAQAIPQTDISGMLTIPSGSGTAIYTVTPTSGLCVGA
                     SFTVTVTVIGINHTISGKTRYAAKANAGNPAPNMPSYNAAIYNIDNVEVKLKSGNTVL
                     ATTMSDANGYFQFTNVTDGNYILAYDHIPYDTMQYVNHVNAVDLALIKYLIGHDTVSD
                     PSRSFTIKHKRAANVDNNASINTVDIARISAKIGLPNDRTRNFPKGNWVALDTSVTVA
                     GADLNITLQTIAYADYDASSSRYKDSLTNWGMAKVLPDENIIIRSDESIIMSDPEYFE
                     VPLRISTKMNELSALGLELSYPSEHYKLVSTSMSNTGKKGGAIKINPALEEIIAANND
                     LLVTDVDGVIRVVYATTDYFDIAANDELIRLGFLSLNDPGLGELDFKLNGTGLIANQY
                     GEINADAYLTMPKIFVQSNDMEAGFEFAGYPNPFSNDATLTYSIPENGTVRLKVYNTI
                     GEMIAEPVNESQLSGKHSVVFSPKNLPAGMYTFKLEYTGLNKSKCVILKLIH"
     gene            complement(10970..14317)
                     /locus_tag="PKH58_00470"
     CDS             complement(10970..14317)
                     /locus_tag="PKH58_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87527.1"
                     /translation="MKRTFLFLFAITLFLTGSVFAQQINVNTYRFNAANTGPTGITPT
                     QLIGPNSDNVASAVTNIGFTFWFAGTAYTQFSVDENGLMKLGGTVIVPEPVNSIASTQ
                     NLPKIAPYWDDLATGTNGSVSYYLGGTAPSRVLYVNWNVTVPKNVAGAANAVIQAQLY
                     EATGNVHFAFGPTYPAANPGGYTMGIGVSATDWATVTVTGNSSATASYGVSANNSNTM
                     TPGNASVKRYQFGTDNTAPTINNNTNISIPNTPGTANRILTTVIADQAGVPVTGIPTT
                     GSFVPRIYYKKNVSGTYVSTPGVLSAGNGTTGTWTFTVDHSLVGGVTTGDQIYYYVVA
                     QDQAGYKGSPNIKSNPVGVVATDVNTIITPPTGVPYYIIGGAYSGIKTVGTGGDFASL
                     TNAGGLFDQINAGQLDGNLTVNIISDLSSETGAKGLNAWVNGAGGPYTLTINPVGNRT
                     ISGATFNGGTANLLSFIGVTGLTIDGLNDGTNSLAIVQTGQYLVGSVIDLKGASNNSI
                     KNLSINGYGASNYAISITNATNASSNNTISHCNITNPVNNANIAGNGIGLLGSATLAG
                     DHNVITGNTISNFVYLALNISGKFSNTEISDNDIYNSLTTSLSNNFKCISIGSSVSGI
                     TNVFNNKIHDILLNSMSAGSIPAIYTSGGTGTTTNIYNNIIYPDIAFTHPAATWYGIQ
                     TIGLGAVNIYYNTIYIGGANITAGNSYGINRGGTGTTNILNNIVFNARSNSTGTGKHY
                     GIYTTSTSLLTSNYNDIYTNGTGGYFGYSTLDRLTLTDWQTATSKDANSLNADPLFTS
                     ATDFLPLNFSLRAGTTVTGITTDITGTIRDVVNPTIGAYEIVCVTPTLVITNPAAVCS
                     PGTADLTAPAVTAGSTAGLTYTYWTDAGATSAYATPATAAAGTYYIKGTKAAGCFDIQ
                     PVIVTVNSCNKILNLSSVMLEGLYSGLGTNLQAYDELGPHWPAGIADHITVELHDEAV
                     YGTIVLTIPDVPLSTTGTATIIVPGTFNSSYYITIRHRNSLETTTATAISFASDTINQ
                     SYGTPTDVFGGNLIQMPDLSYAIYGGDIDQDGFIGVSDMASADNQSAAFGSGYLPEDI
                     DGDGFVGVADMAIIDNNAANFVSAILP"
     gene            complement(14361..16775)
                     /locus_tag="PKH58_00475"
     CDS             complement(14361..16775)
                     /locus_tag="PKH58_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX87528.1"
                     /translation="MKIKYFFGFLWTMVIIHISVSIQAQNPTYLCELRNDAQVSCNIF
                     EFDIYLIRTGTIPFEYATGQYGILVNPLFKNGGVITASIVAGSSDPALILTNQNPTNI
                     SFLNASNCIRIAGRIPPGSGNGAIISNGSPGTKICRVRLTNTVAFGQFTPNLTWTTQA
                     IYPTQINAYVGGSNTTIMLTASQTTNNLNNVEPAGIWSGVVNSDWGNPGNWLCGAVPT
                     SSTDVIITSAAQSPIVNSAGTARCHSITIHSGASLSVNSGSDLSVYGNWTNNGQSNIG
                     DGTVIFLGGLTQSINGVTTFGNLTISNLNGVTINNNTQLTGILTPSSGVLASNGHLTL
                     LSTASRTALIAGTGSGSVTGNVTMERYMENRLGYHYYSSPFTGAPINEFTDELGTLIT
                     GNPYVGNDTTHTVTPFPNFFMYDEPLGPSMSIGWTGAGTTMTPMRGYCINFGASTAPL
                     TADVTGVVNSGALNFSVTKSTTGNNYADGWNLVGNPYPSPIDWQASSGWTKINVSDGI
                     YYFNPNTQYTGTYSSFVNGIGIPSGTTGIIPSMQGFFVKATANGILGVNDAVRTTDLT
                     PSFNKTLENPDALLRLKGYPAMNELHADETVVYFDPLATFIFDGNMDAYKLLNNDPAY
                     PNIFTKDSSNYSLSISALPSLSNTDVVIPLGFTTKTSGSFTIKATEILNFDPSFHIYL
                     EDNQNSTSQDLTVSPEYTFTINANAPLFRFFIRFSPTIVTGIEENSNSFVDAWSSGKY
                     INVNYSNSTQGNAFIFIYNMLGQKIINDEQTGIGTTHYRIETPGCYIINVLSGSKNYQ
                     KTLIIK"
     gene            complement(17279..18289)
                     /locus_tag="PKH58_00480"
     CDS             complement(17279..18289)
                     /locus_tag="PKH58_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNX87529.1"
                     /translation="MINLKTKYMGIELETPIIVGASSLTANLDNLKKAEELGAAAIVY
                     KSLFEEQIQLENFQMDEKLTEFNDLNAEMITTHPKLEHAGPDEHLWNIRKAKESLFIP
                     VIASLNAVNNVTWVKYARLLSETGVDGIELNFYQNPTDFTKDAQEIEDEQIGIVKDII
                     QNISIPVSVKLSSDYTNILSFIKKLDQTGVKAFDLFNSFFQPDIDIKSEKHIKSFNLS
                     NEGDYKKSLRYAGLLFENINADVCSSHGIYTGEDLIKLLLSGATCAQVVSTLYKNGLT
                     QIKTIQNELVEWMEVKNYNTIDEFRGKLSKNKLSSNPFIYKRAQYVDLLINSDEIFGD
                     AK"
     gene            complement(18340..18684)
                     /locus_tag="PKH58_00485"
     CDS             complement(18340..18684)
                     /locus_tag="PKH58_00485"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HNX87530.1"
                     /translation="MMHIAADFILQGNRLYKLKASKTGFLLAHVGIYTIFFIVFSPLL
                     LELTFVQGLVFSAINGGAHLIVDFFTSKFKKLYWQKNEAKYIAVISIDHVLHISILMG
                     TYIYMYPQLLAG"
     gene            complement(18868..19554)
                     /locus_tag="PKH58_00490"
     CDS             complement(18868..19554)
                     /locus_tag="PKH58_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX87531.1"
                     /translation="MKILLVEDDTKISSFVKLGLEDSGYDVSVAYDSAMAEKIVFSKE
                     FDIIILDVIIPGINGYELCKKIRTANIKKPVLMLTSLDSVEDKITGFDCGADDYLVKP
                     FHFKELLARIKALNRRHTDAVVIPIIQKIADLELDSISKKVSRNGKEIKLTAKEFAIL
                     EYLISSPGKVFKRMEIAEKIWGFSFSGTNLIDVHINSLRNKIDKDFSPKLIHTIVGFG
                     YVLREEEIQH"
     gene            19766..23701
                     /locus_tag="PKH58_00495"
     CDS             19766..23701
                     /locus_tag="PKH58_00495"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX87532.1"
                     /translation="MRKLKIKGLYQLFVILLMITLFLTVTSIVIIYKIVYQEKRVYLR
                     ELCESRKEFIESIYQQTNNPAFVLEVLYKQQRANDGLGISGEFIIGIERNDSIQFLYK
                     SKQKVEANYTVIPKTSDKGIPLQYALSKKTGFIQGIDYQGVLVLSYCTYIPELKWGVV
                     TKMALSEVRLPFYRAGFIAIIASFILFLIATIFFRKISFSIKRKIDDSEEKLTAILNA
                     SQESIFMFDQKGTIIIANDIASQRLKKTLPEIIGKHFSVFIPEEIVHERWAYLTKVFD
                     SGKPYQFEAYRDDAIFEHNLYPVFENKKVAYVVSFSQDITERKGMVEQKEFHSKVMET
                     MHDAVIATDTSFIITVWNNAAEKLYGWSRKEVLNKNSRDILKSTISDDDLKKMLSELK
                     TDNNAHYETIQHRKDGTPITIEARLSVIRDEKGEIVSYIAANRDISERKAAEAALQKS
                     EASLKRSQEMAHLGSWDLDIKTNIVIWSDEVYRIFGMKPQESDITYEIFLSRIHPDDR
                     DSVVKAYSGSISEARDTYEVDHRIIKKNSGEIRFVHEKCSHIRDDSGFIIRSVGMVHD
                     ITERVMYEEALKQSEESVRLKLQSIMSPEGNIEKLELEDIIDTQAIQSLMNNLHVLVN
                     IPVAIIDLKGKVLVGVGWQEICTKFHRVHPETCKNCIESDVQLTRGTPEGEYKLYKCK
                     NNMWDIATPIIIGGEQKGNLFMGQFFFDDEQVDHAYFVAKAKKFGFDEKEYISALEKV
                     PRLSRQKLEYAKSFFREFAHSLSQLSYSNIKLARSISEQDKIAKKLNDTNNYLEALIN
                     YANVPIIVWNPQFEIQVFNKSFEHLSGFLSEEVIGTKLDFLFPPESLTDSMEKIISAS
                     FEKLESVEILIQCKDKEAKTVLWNSANIYDTDNKTLISIIAQGVDVTERKRIETALKE
                     SEENYHRLFEFSAIPIWKEDYSEIKKHFEALKISGVTDFRKYFETHMDEVIHLASLIK
                     VVEINHKSLEFYGAESKEDVIKNMLFYFNEESLNVFREELIALASGEKKFECEMPMRT
                     LSGNVMILDLHLSVVKGFEDTLSNILVSFIDITERKRFENELQEKNEELKETNATKDK
                     FFNIIAHDMKNPYISLLGASELLYENAKKYDRDKIITLAKILNDSAKSGYDMLLNMLE
                     WARSQAGSMIFQPEKINLKALIDKGLSNLIEYAGSKKVNLKFDIPDSFQVYADKNMLT
                     SILRNLITNALKFTPEGGDVIVWTKEENRSIIVFIKDTGVGIEKADLSKLFRSDIKYS
                     HPGTNHEGGTGLGLLLCKEFVEKHGGKIWVESQVRKGSVFYFSIPEKIV"
     gene            complement(23971..24552)
                     /locus_tag="PKH58_00500"
     CDS             complement(23971..24552)
                     /locus_tag="PKH58_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNX87533.1"
                     /translation="MKFELPVLPYELDELEPFISRKTLEYHYGKHHQTYISNLNNLIS
                     ETKFENAKLETIIMQAEGPLLNNAAQAWNHTFYFMALNRNNKSLPHGILADAIQRGFG
                     TFLDFKDAFTKAGVSLFGCGWVWLVKNQNGQLEIIQEINAGNPLRKGLKPILTCDVWE
                     HAYYLDYQNRRNEYINSFWSIIDWTVIEKRFNN"
     gene            complement(24697..32715)
                     /locus_tag="PKH58_00505"
     CDS             complement(24697..32715)
                     /locus_tag="PKH58_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX87534.1"
                     /translation="MKRILSLTLFLFAVWQSALADNYYWVNNGGNWTDVSHHWSKSSG
                     DTVNMQSVIPGPNDTVYFDANSFTQSGQSVNLDIPVAECAMMDWSGISFNIYFFSGNS
                     DTLKIYGSCILSNNLNFNFYGVLVFMSPPAQVSDLDFANKVLACDIVLSSDSVRLLSR
                     LYMPYNQLSLVDGSLFTNGHSVTCQHFNTDSTLKNSIPVANPKWFSNDTLTIRGSMAL
                     SDSLDFLQNGPVYFAYNSLDTNYFNLYLNTLPGKVIFNGSKNMFLRSGLSVAGSIEIT
                     GGGKFYSKGYPLNCAAFTSATPLTRTINLSTSLITLNAPGNALNFQSQGLTLKADSAI
                     IAFNYSGNDTVIIRTGKDTNNISIFKEIHLPSSNAMIFNSFNTSLLAFGTGSNIALAT
                     SITITLDSISSAGDCGRYNYIKAFCPSCIQCENVQGCGTLLPNFDFNYPDTTDYLKLS
                     NIKASGNPVANNSFDEGNNTGWTINEPATPPTLYWVGGSGNWNDPDQWSAVSGGTAHS
                     CIPTKSTNVVFDNLSFSGSNESITVDEKAYCQNLMWINTAYHDTVKGNGALFVTGNIV
                     LNSYSHFKLAGGYRLQNPNTGYRTITSNGADINGDIEINSSYEWRLSDTLNLKGKINL
                     VRGTLNLDNNDMRCHAFLSEGNEIRTLNYSNSVIYLNGSDTAWKSAGSNLALLHDSSS
                     IRLVGAGPDFMLFDAENRDFDTLDIRCPKARIAGGMDCRLFKIRPGTLLECEPLKTVK
                     FDSIQASGSCDYPITIGNYSGSVDTALFMVNSNDTINIINVEHVIISNVCAVSDTGHI
                     YNALNSIGLNKFSGWTITGSVSGNTYYWTGLQSKLWNDRLNWEVNNATALCIPGPMDT
                     VVFDSIHFSVPGIHDTVIVETNAYCKTMTWDSTINRSPALLLNGDLTITGSVRLCDTL
                     IVDYTNNFQPTDINAPHFILSPQNANCWFTPCKEFNVNLNIEGRNISDSVFLLKNLYM
                     DTLSTVTFLSGTFSSNKKNLYAGLITTVGNKIKNVDFSHSQITAAYNFVMQNGSLLNL
                     NMDSSHLVMDDNQYYYNLFDGGGQDYAGLEFRSRAPDTAGVYYKAFVKSSDTFELLKL
                     NPGIHLELKAGLTVAFDSIILNGTCLDSIYLQTTGTASATLHKNGTTGFAGKFLNVKK
                     ITATPAGVSALFSKDLGNNSGWVFDTSKYVTSSFSLPATTCFNDSIQFTNLSTTHGNP
                     VDMDFIWSFGDNDSSFTFQPSHLYANNTSYIVTLISTDTATGCFDVYKDTTLTIYKPE
                     VTLNSSEIDNSICLGDSVFFNAYSSNPSPLFYEFIVAGDTVQQSPDSIFATTSLADGD
                     EIFVILTYQGCADTSNHVIYTVNSLPVVHLASNDPDTTICAADTIIFTGSGADKYQLY
                     RNDTAYGAFSTSNQWIIPAPGNGQQFTVYGQNTTTGCSAGSTDTLTVTVHPLPDVAII
                     SSDADLSICYGTPVVFTASGANLYQFSVNGMAQGIFSSIDTTTLTTLSNGDNVMAAGI
                     SSDGCMAFSDDYLEFTVMPTPVVSLTSSDPDNIICSGDLINFQAFGADQYQFSVDGNI
                     IGPYSNSDNFSTDSLTNGQVVLVTGTLGICQSQPDSIVMDVRPSISWTFSANEICAGD
                     VVQFTAHGDSLYNFYINGVWDTVLVDGSIYTATGLSNGQQVSVSGTAGACLPSPVTVT
                     VNPLPVAPMACSDQDTTICAGEIITFTGSGADKYQFFVDGDSVGAFSFFSTYSTDSLT
                     NGQVVTMQAMSAQGCTGTSPNSYTVSVNPSPMVVLSQSDPDTTICSGDMVTFTASGAD
                     TYEFFISGTSQGAAGPDSVFSSSAIFNGAIITVNGTTGLCTSTSSNIYTYVVTPTPIV
                     TLTALTPLSYCSGDTLRLLAGGATTYEFFVEGISAGAPAANNMFISNSLTNGQTISVA
                     GYQSGCFSPGNNDYTVTVNNYPSAVLSATPGGPAFCYGDTVIFNGSGAQNYIFFVDGI
                     PVSYDSVFMTDALEDGQVVAMRGGNGNCWTDADTTIIVDINYINITLGCSQPSGVICA
                     GDPLTFTANGANLYEFSIDGVSQGSPSASNTFTSASLINGQVVSVEGTSLGTGCLQNA
                     FENILVHVFSVPQITVTPSPTFCEGDSAILESSVTDGLTWYHDGAVITGETSPELHVF
                     TEGLYAVQSATGGNGVVFSAGANYFGQLGEGTLTNALDFKEASGMNSIVEVVCGAEFT
                     LALANDGTVRSWGRNEFGPLGNGNYTDSPLPVAVGNITNATHIAAGKRFGVALLQDST
                     LVSWGENTQGQLGYGNYSTSNFPFPVIGLSDVTDIAAGDNHCLAVTADGKVWAWGYNH
                     FGQLGDSTLLTRNLPVQVNGLDNVVKVRAGGNHSMALKNDGSLWVWGANNSGQLGNGT
                     NTSSMIPVKVNLPLPVAVFDGGFGHSIAADTAGHVYTWGDNSKGQIGNQSSINALYPV
                     KIIKAGSARQVRAGQYSSYVLRTDDNVFSWGFNKNGQLGQQDTSAVNEPKAITSLFGV
                     VSFDGGNNHCALVNDQEHSCISGNVQIHVDTVPHVPVFLNGLTLYTSAQGTQYQWYYQ
                     GSIIPGANDTMITIAAEGSYMVLVGFANGCSAFSDEYSYHVGLEEASQNKIFTLFPNP
                     NQGSFYIQFPGESDIFSSLKEIRITDMPGRIIHCKTIVKDVTSIRIELPGIVNGVYVI
                     ELQFNDNSVLRKRFVVEK"
     gene            33020..33634
                     /locus_tag="PKH58_00510"
     CDS             33020..33634
                     /locus_tag="PKH58_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87535.1"
                     /translation="MKKQFFIIFTLCLSFTTAFCQVQTTPIDSTFYKQVQSGSGNSTE
                     FTLKPTTTYDSIGIPVDVNRDLTDTLTLLPGTHAKLKLPRYFEPFTYEKFSGFLHKGT
                     STSILANEYQGMAYTTMTSQLTDSTFSKQGAELLETFDLKQVDGTPAKVFIMRIKTKT
                     APVIRIMYFAGNYQTTYNLIANIPEVVSNLIRNVILESFRTLEY"
     gene            33645..37535
                     /locus_tag="PKH58_00515"
     CDS             33645..37535
                     /locus_tag="PKH58_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX87536.1"
                     /translation="MKKIIHFILIPLIFYSFGLNAQTTTVTYPAKFHTENQNYWGPNA
                     TEVNAHEIFLDYHLPVTTLGWNYVYNLAGGQYGVALGAFTWVNIGAGLDIHEDSARVK
                     VDYLADVKLQMPDAGTFDKGDVVTIHTSLDPVEDSCKIEYDPYKLDMGLALDFGLGIW
                     IGAQACWGSCEGITLYQYDLPAFSVSLMNLSSGSLTIGNQTINAEIFPDMPPVLTNYL
                     YTHDSLSLGIPTMPFKIVLKYPSLHSDTNDTFLSGDTLICQSARPFKYMQLDIDVIKL
                     IGKILAKSPEPVTQTAGQILSNLSNNIDLSIIGIPVHVGYSIIRSGIRLKMYHNRKLK
                     FIPTISSQMKFPTVVDYKVVRPDNTIASQGSDSLINYKVGEDIRFTYPCNYDFMNVSS
                     SYSMTNQFTNHTWDKYLMNFFVEMLWINLELPSVTIIPEICIPVYYPCGPWYCYVCDW
                     CYGGDFCTPPVVFPGVDFEAGPLVNWELPINWKSFDWTNNTWALNGFNTYLAPVPFTL
                     TPRPFTVTATSIPINCYGQNTGSATATVTNGKPPYTYEWSNGVTHTSVNTTDVVNNLG
                     PGTQYVIITDYNGCQTFTDVIVNQPSAALSVNANIIQADCNGASTGSIDLIPAGGTAP
                     YTYQWNTGQTAEDIATLLSGNYIVSVTDHNGCVSVQNYTVQQPPPLVLTPAFSNVNCN
                     GDTTGTASVLVSGGVTPYTYLWPSGETVPDVTGLSAGSYTVSVTDFNGCMKTQDIQIA
                     QPAAPVSLSVSAQNALCYGDASGYITIIPAGGTAPYTATWYNPSGQILNQNTVSLDSL
                     VAGTYNAVITDAAGCTTDSSILISQPSAVTWGFSTVNNLCFGESNGSAVITATGGSAP
                     YAYSWSNGSTDSTATNLHAGTYYVTITDNNGCVQATSVTIMEPASAVSATVAPTHILC
                     FGGSGGMANLNPIGGTPPYTYLWSNGFTGEDLTGVPAGAYTVTVTDNNGCITYSGTVI
                     NEPADSLSTQLSIVNPSCHGFNNGSISIQASGGTSPYYLRWDDTDFLLSATGHLLTGL
                     PAGTHQIIVTDANGCQAIQLVTLTAPEEILLDTVTTIVNCFGGSDGAIDLTVSGGTLP
                     YTYLWSNGEVSEDLSGLPAGDYSVTVTDAQGCITNFTSRVGSRPEIEISSTTVPISCI
                     DNNDGSIVVISSGGTGNITYIWSNGATEGNIHDLLAGNYAVTLTDALGCTKNFEFTVP
                     SSLFECIFIPSSFTPNDDGTNDTWIIRNIDLYPENIVKIFNRWGNLLYERSPYNEPWD
                     GTYKGSPLPSETYYYIVDLNNGTKAFTGTVTIIR"
     gene            37544..39997
                     /locus_tag="PKH58_00520"
     CDS             37544..39997
                     /locus_tag="PKH58_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HNX87537.1"
                     /translation="MKRITFFLLLIAASSFVFADKHPENTNDGNSPPPCHTALLSKTN
                     VSCYGGANGTATVTISKAGTYRVIWSNGVTINSTTSLTHQITSLPAGYYDVQVIDLVN
                     GCAAFDIIEVTQPALLTTSNTSVDVKCYGQSTGSINLSVSGGTTGYTYSWSNGYTSQD
                     ISNIAAGNYLVTVTDSKGCKAYDSAVIAQPAQAIGQSLNIVNPKCTGSLDGSIDMTVW
                     GGTAPYTYFWNSTYLSQDLNNIGAGNYSVVITDANGCILNGSGTLTNPQLLAASLTFT
                     DNLCYGDMHGSIDLTVSGGTPPYSYSWANSDFMLSWATQDVSNLPNDKYYVTVTDANG
                     CYRVDSALISSPSEIVSTIAHTDVTAFGGMNGSITLSVTGGVLPYSFSWSNGSTSQNL
                     SGIPSSWYYVTITDAHGCIHQDSVFISEPLSALVVDIIAQDATCFGYSNGSITSTTAG
                     GTPPYSFLWSTGASSEDISNVSAGIYTITVYDFYGNSASDTVEVFQPEAFTFPYTVTD
                     VSCNGLSNGAIDVSVTGGTSPYTYEWMNSEYVLAALTEDITGMPADQYYLEVTDSLGC
                     TGSISITINQPNALTIQIDHTDAICFSSATGTASCIISGGTPPYNYYWSGGQQTSDLT
                     NLVAGIYYVTVTDANGCIITDSVVISQVDSLTVQYVMSPVSCTDQHDGKISITVSGGN
                     GDFTYQWSNGASLPDIEELYAGEYEVTVTDFMGCTAIKSVTVTKIEIDCINIPTCFTP
                     NGDGFNDTWHIKDAELYPGFFLEIYNRWGQLLYSLNGTYEQWDGNYKGKPLPSETYYY
                     FIRISPDSPVLQGNVTIIR"
     gene            40010..40939
                     /locus_tag="PKH58_00525"
     CDS             40010..40939
                     /locus_tag="PKH58_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX87538.1"
                     /translation="MKKIVTLVVLLFAFNALNAQQLSLTSQYMMNHYVLNPAIAGTAD
                     YIPINLNIRNQWVNIKGAPVTQSFSAHAYLGKNIGIGGCFFNDVTGPTRRTGMSVSVA
                     YHLRLSKDYSRKLSFGIGGVFFQHYVDRDKLSTDVPEDMSILDGFNNQFCPDVNFGIY
                     FSQTEKFYAGFSVLNLFEIKKDLFTLSNRIKNPIKRTYYFNGGYFFDLGKDFGVEPSL
                     LMQFQETTPLQVDLNARFSYKKMIYLGASYRIKDAVVIMFAFNMKKFAIGYSYDLTLS
                     DIKKHSYGSHEINLTYRIFKKDAFKKDYNYPLF"
     gene            41021..42796
                     /locus_tag="PKH58_00530"
     CDS             41021..42796
                     /locus_tag="PKH58_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87539.1"
                     /translation="MNKANELIDVGNYTEAAFILESVDEKGTADQSALVNLAYCYIRM
                     HDYNKAAQIYPRIIAAKKIDQIHYFYFGEVLRSLGKYSEAKEQYNKYLKKFPGDFKSV
                     TCIRSCDSLVLWKSLSSDNKVENANDINTSFDELCPVSTKDGIFYISNNKDLLVNAGA
                     NTDLNDPQVSFIFQKKEGQTTVFRPYSDSISYLAYSVRNGKYAMVVKPVRKTPDGFFA
                     GTPVLKLSNDNKAWNNFQPEETPEGYTITHPCFSEDGSRIYFASDIPGGFGGSDIYYS
                     DYAGGKWNKPVNLGNNINTPGNEMFPYITEKGDRLFFSSDGHPGYGNLDIFESVYTYG
                     NWSNPRNLKSPVNSVGNDFGFISGKDAYHGYFVSNRYETSVGGNDIYTYSFVMPEPKD
                     TTPVLPPSRPFISDTVFVFFKTASSFIDPVFTSLLDSIAGIMADDPSLKLNVVSWADV
                     HGSDSINKNICNERAGAVANWFTSKGVSLGKIIKKPSGVSRRPEIKQIDYHVQIGILA
                     KAGAQDYYSQIIKNEALVDCLKSGNKYYYYAGNGTLDEMKTLQENLKIKHRFSGFICA
                     SYCNYFLLDEKYAPNRRVDIYFSKN"
     gene            43252..>47908
                     /locus_tag="PKH58_00535"
     CDS             43252..>47908
                     /locus_tag="PKH58_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87540.1"
                     /translation="MRKSLLKNAFLFVSVCLMMPLLSFSQFVENNGQIAHREISKLRP
                     DIKFQVKGNEVISNYKADGIENYYCKVREVPRENWTDDMKENERMDRKVRSFQDIFFY
                     RFDVAFIGSNPDVSIEPSDKSKTYRNYYYEFCPDGILNVPEYKTIEYQELYPGIDLRY
                     VGTDKGIKYEFILAKGAKISDIKMKYIGVDGLNVEENGDLTYYIDGKKMTEKSPFTFY
                     SGSGKEISSAYTLIQDDIIQFKVDGIDEVTEDITIDPVLTWSTYFQGVYGGYQGDPGV
                     DNQGNVYICTEEGAGTLPVLNAGAGQWFQIASTGPSPNWDLAIFKFNSDLSLAWCTFY
                     GGQACEQGWGKRNVAIDNTNNRIYFSGETDTPYSNSVQTFPTYNPGGGAFYQDVSYDN
                     GSSYPFFLEFTPAGVRSWATLFTYETAPFSGGMGTDVESIYFRNGYLYFSGETNRYGH
                     SGFPLRTLAGAYNNSYWPGDGQHIYCGRFNSSRTLEWCTYVNNNVATTSFSSNLGDMV
                     VDANNNIYLVTDYSEYSASKLSTILPLVNPGAGAYFQATGGLGYGSRRGTHITKLNPS
                     SSTVLWGTFYNGGYGDLPGVCEVDASNNFYVGWRSTGSTDFPCYNPGSGAYYQSTKRS
                     VCLTGPNDYANGGKDGAVAKFTSAGVRLWATYINGTTPTSGETGVNDIDTYPTGEMMM
                     SSKTVCSDFPVQYLSGSYNKSTITTSGTYENVFTKFSSGGVILWSSYFQPLMGYGAIQ
                     NNNCVSKYFVDNGGGSTIPLVNPGSPAYYSSTPQNYQSFQLFEDATSNQSTPASSISA
                     SPTTTICTGGSVTLTVQGGSLGSGASWQWFSGSCGGTFVGSGASVNVSPTSNTNYYVR
                     ATGVCNTTSCVSLLVTVTPNNTISLSAGGTQTKCINTAITTTTYATTGATGATITGLP
                     TGVTGSWASNTVTISGTPTAAGVYTYTVALTGGCGTTTTTGIITVTPNNTITLSSGNN
                     TQTRCINSAIANISYTSTGATGATFSGLPSGVSGNYSSGNITISGTPSASGTFNYTVN
                     LTGGCGTVSATGTITVTPNNTITLSSGNNTQTRCINSAIANISYTSTGATGATFSGLP
                     SGVSGNYSSGNITISGTPSASGTFNYTVTLTGGCGTASANGTITVTPNNTITLSSGNN
                     TQTRCINTAIANISYTTTGATGATFSGLPSGVSGSYSSGNITISGTPSASGTFNYTVN
                     LTGGCGTVSANGTITVTPNNTVSVASSTPTLCINTPLTAITHTTSGATGIGSATGLPA
                     GVTASWASNTITISGTPMISGTFSYSIPLTGGCGTANATGTITVIPNNTITLVAGGTQ
                     TKCINTAITTTTYATTGATGATVTGLPSGVSGSWASNVVSISGSPTVSGAFTYTVTLT
                     GGCGTVTANGTITVTPTNTVTAASSTPTLCINTALTAITHTTSGATGIGTSSGLPAGV
                     TASWASNTITISGTPTASGTFNYSIPLTGGCGTTNATGTITVIPNNTITLVAGGTQTK
                     CINTAITTTTYATTGATGATITGLPSGVSGSWASNVVSISGSPTVSGAFTYTVTLTGG
                     "
     assembly_gap    47909..48008
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <48009..>50014
                     /locus_tag="PKH58_00540"
     CDS             <48009..>50014
                     /locus_tag="PKH58_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87541.1"
                     /translation="PTASGTFNYSIPLTGGCGTTNATGTITVTPNNTITLVAGGTQTK
                     CINTAITTTTYSTTGATGATVTGLPSGVTGSWSSNTVTITGTPTASGTYTYTVTLTGG
                     CGTVTANGTIMVSPINTITLIAGAPQTGCINTAINNITYATTGATGATVTGLPAGVTG
                     TWMSNVVVITGTPTVFGSFTYTITLTGGCGSVATTQTITITPNSTVTLSSASGTDAQN
                     ICNNTAITNITYATTTATGATVTGLPAGVTGSWTSNVVTISGTPTVPGTYTYTVGLTG
                     GCGTATATGTLTVIPVNTITLTAGGTQTKCINTAINTTTYTTTGATGATVTGLPAGVT
                     GSWTSNVVTITGIPTVAGVFTYTISLPGGCGVSTATGIITVTPDNSLTLSSGAGTNAQ
                     NVCINAPITNITYASAVATGAIVSGLPLGVTGGWASNVVTISGTPTVTGAYTYTVTTT
                     GGCATATATGTLVVNVNKTADVSVQAFDNPACSHTMVTFTATPVNGGLSPAYQWFVNG
                     NPVGGNSSTYSNISLSNADAVVCRLTSNEFCAINNPAYDTVNMIVNPIPIAEAGTNAT
                     YTGTPILIGDPLNGPGVISWSPATGLNNTWVAQPLASPSVTTTYTLTINNFGCIRTDT
                     VTVHFGGLGHVISGKTRYLKKGTSGTPSPNPPTYNAVIYNIDNV"
BASE COUNT        14303 a        10112 c        10428 g        15071 t
ORIGIN      
        1 cattttcggc actggcacaa tgccttacgt atatcgaaaa ctggaacatg gcaaaaccat
       61 ctgccatgat atctttacgg aaccgggcgg caaccttgcg ctccttaatt tcaacgacag
      121 gaaggtcgaa aaatacaagt acccacatga tacggtattg gtttaatttg cttaacatgg
      181 ctctgtttgc agacttgagt ccatcggacg gttaaaaatt taattccggg taaataattt
      241 ttttagaact accttcaaaa catttgaaca aggatgccgt agttcgttgc aggccaacca
      301 tcagcggact gttttcacca tcgatactga tatccatagc cggtatgctc aacaactttt
      361 gcttgatatc cttattcagt tcagtaaaat ctatttcgct gtacacaatt tcgcaaacca
      421 ctctgtcgac catggggcgg tatggttcca tgatgtcgtc ggccaggcaa taggcattgt
      481 acttgttttc gtgatgaatt cccaatgtgg gcattaaacc tgtggccact aaaccacggg
      541 caaccacagc gcgtaaaatc gcataaccat aattcaaaag gttgttgggt ggttcaccct
      601 cacgaaaacg tttaaatcca agtttttccg gaaaaatatg ctgccagtaa taggcggcgg
      661 cctgagcctc ataattgtca ggatcgccgg aacgcacatc accggcccat ttcatcagcc
      721 acgaagcgtc cttcccgtat tttttcagca tggctgcctg gttgcgtatc tttgaaatta
      781 tggtttgctg ccacaattgt tttttcaagg gaacgctggc atcaatttgt gccctgaaac
      841 gctcgttttg catgctgttc acatttagcg gcaaaaacag tcccgacggc atgtggtttt
      901 ccgaacaact gatcaatgcc acgttattat ccaacaacgt gctgatcaat gcctgtgtta
      961 tggtgacctg tttatcgtcg agaataacaa ccccaatatc ttcaacagga atagatgcga
     1021 tagcttcttt ttttaacatt tcaggcattt ctgcattttt ttctacctgc ggcaatttca
     1081 cgatcagttg tttgttgtcc agatgcaggt atgccggatt gccgaaatac aaggtgcgtt
     1141 ttatcatttg aacatcattt taatatttaa taccttatgg caggatatta gttaaatgga
     1201 attacggaaa attgcgtaac ggaaaaattc gtaattgtgt tactggtgcc gtatgatttc
     1261 ttcttttatg ttttcatttc tttactaatg gtgactcttt gacaaaatat tattagatca
     1321 gtccacattc ttttgccctc aaaaaattta taaagctatt gtgccggttt tcataataat
     1381 cgcagtacga tgagaactcc cattcattgg catctttaac cagacctgcc ttcacggggt
     1441 tgttgtgaat ataattgaaa caaagcttgg gatattcttt atccgggtat ggtatattga
     1501 tgtatgcgcc atgaatggta tcggaaaagg caggtgtaat cgttgaatta tttgaaagac
     1561 acgcggcttt tgtatgcggc ttgaataaag agccgcttcg gctttcctga atattaatag
     1621 cacgggtgta tgaacgaagc atgatggcga tggattgtac gagagtttgt ttaccctcac
     1681 tcatctgatg actgggctgt gaagtcatct gatgactcaa agtcatctga tctgtagcta
     1741 tttgggggga agtcatctga tgactcaaag tcgcctgatc tgtagctatt tgggaagtca
     1801 tctgatgact caaagtcatc tgatgactag tgtacaccat cagatgaaaa tgatttggca
     1861 tcagacaata ggccagcaca tctgcaaaag gtacaatatg cgtttcaatt tttttcagaa
     1921 agaaaaaata gttctctctg ttaaagaata tcaactcttt gttattgccc tggttgaaga
     1981 tatggtagct gtgttccggt tcaaaatgca tgtgcagtat tctttatgct atcacttatg
     2041 gcctatgttt ttctcaagca cttaaaaatt cactttgtta ttgctctgaa aggacattta
     2101 tttctttctt tccctgatca aattgagttg ttctatatct gctttgtctt tgaggcggcc
     2161 ggtgattatt ttgtttgtta tcaaatcctc caatttcagg atgggaattt cattatcgtc
     2221 aattttaaga aacaccctgt tgttaaaggc ttcttcgaaa tttaatccat ctacacgggt
     2281 taaaaaatca atacggtccg gttctgcgcc tatatgaaat atcacagtgc cgctgaagtc
     2341 taatttttca ataaggctga tgtcgtcagg attgaatttc aattgtgtaa atacttttat
     2401 cagttttgtt ttgttgtttt cgctggggcg cagccatacg tccatatctc cggtagttct
     2461 tatatagccg tgatatatca cggcatatcc gccgacaagg ataaaatcca ccttgttttc
     2521 gagcaaagca gcgagcattt cctgatgtgc cgggtgtgtt atattcataa atcaaggatt
     2581 attttttttg ttgaccaatc gggtttgaca aatttgtaaa agcggttcat caattccaga
     2641 aacctttcaa atcgttcttc gggtgtgagt tttgcccagt agtcgagttg cgaatttttg
     2701 gcatcttcag gtgattgaaa tatagtgatt ttttgcgttg tagcataaga attgacaggt
     2761 tctttagcag tattatcact atcgtatgaa tttctatttg atttatttaa tggattggtc
     2821 agtgagatat tcccaagaca gtcaattttt attttgaatg gttgtagtga aaataatgct
     2881 ccaagacttt gaacttttat gaaacgcctg cttgctttag catttgaatc atcaattaat
     2941 tgtgtttcta aatgatgtct gaatacaatt tcaatctgtt ttgctaaagg attacagaat
     3001 attttttgaa ctctatacaa tttgccactg atagccatgt aatctttact ttcaagagca
     3061 agtgtaattt cttcctgtga catgcctaaa acaaacattt cattttgctg catacttaaa
     3121 ttcaatttcc agtttgccag aggcagtttt acaagaaagc tttcgggata agtatcttcg
     3181 ggagcttctg ctattttgtc ccaaacctgg tttgcatcgt ggattataac cggaatacca
     3241 tatttttttc tttctacggc atgccagaag gtgcaaacat gttcaacatg gtttccttca
     3301 gaatctttat aaatggcaat atgatgatta ttgcccggtt ttacgaaacc aatatttttc
     3361 ccgttttcat ctttttttac aggaacaact gccgaaaggc cggtgaaaca acgcacagat
     3421 ttaatggggc gttctaaacc ttcatcttcg taccatttca ataacgtttt tccttcatgc
     3481 tgcacgtctt taaatgcttc ctttggcttg ttgttaaact tattcaaacg attttgtaaa
     3541 atttctttaa cttttaaatc aataacctta tcaactttgt tgaagtctgt atttaccgta
     3601 tatttaatta caaattcttc ttttaagcaa gaagcatatt ctaacaaaat ctttttttct
     3661 ttatcaaggt atattggttc cttttttaat gaagcaattg ctttttttgt atcaccatca
     3721 taatgcaaaa gcctgttttc aacgagttct ttgatatatg gtttgaagat caaatgaggg
     3781 ttttcaaata aatattttac aggttttttc tcctcaatac ttttaatctt tccgtaaacg
     3841 ctttcctcgc tcagtgatcc gcgaggtaca atgattccgg tctgaaccac ttttttatta
     3901 ccttttttgc cggtttttcg tgttcctttt acagccactt ttttccctgc tttaaacgaa
     3961 acaagtatac ctgaaacagc attttccaca tcttttacct caattggttt ttgactgatg
     4021 atgtattttt ccaataatga acgtttttca ttgaaaagat cagaacactt ttcaacctcg
     4081 gcatacatat cctcacgtgt tttgcttgaa cttagtgaat taaaacgttg aataaatccc
     4141 tgcctggtac aggctatcgt taaagcatca acagcgtggt ggcggtggtc atcacgtttg
     4201 gtccaatctt taataacttc ttttgtatgt ttgttttgtc catgctcgct ttcccattcc
     4261 ttagtttcag ttaatcccaa atctttatat tttggcattt gcaggttcat cgtcacatca
     4321 tcccagcccc ataaatggcg caattctgcg gtaacatttc cacttgtact ccagacgtta
     4381 tggcatatgg attgtaaaat ttctctcgct tttgtagcaa tgtattgact ttcgcgtaac
     4441 tgtcgatcaa taaaattttt tgggattttg ctttcagtca tcagaagttt atcccgtttt
     4501 gcttttccga tgatatggtt atcatacagc atgttgaccc gttcaacata ttcatgaaat
     4561 gcttgttctg attttccttt catgaaatca taggcagtct ggtcgttttt attcttgtta
     4621 caatgacggt gagctaacgt cttattgctt tgcgaatcat caaaaagttt tgatttagga
     4681 atgatgtgct caatgtctac atcattgcct ttaatggctt ctgttaatga aattggctga
     4741 ccgcaatata tacacatcgc gttcattttt ttatcttgtt tatctatctc ttcatataaa
     4801 cgccatttaa tgatattgtt tcttgttgct cttaatccga attcagctaa gcttttggca
     4861 tatttttcat tttgtctttg cctttcattc attgccttgt cagtatcatt cctctcttct
     4921 ttactttgtt ttaattcacg ggctaattca actcttattt catcaggttt accatattgt
     4981 tcaatggccg cattaacgac attaaccatt tgattcaata ttttttcaac aatgggctgc
     5041 cgtaaggagt tttttgcaat aggttttaat tgatctaaaa gttttctttc taaattttgt
     5101 tctttcgtaa gagaatttga atggtcataa cctgcataac tcatggcttc gctatagcct
     5161 gctccttcca ttaaataagg taatatttta cgaatggctt tggcggattt gttgccaaaa
     5221 gcgaattttg caaaatcaat acctgctaat ttgtcagcag taattttatc aatattgaat
     5281 ttcttttgca gaacagtact gcattctgcc tgatcgttaa ttgaataaat ggtgtgccag
     5341 agttgataaa atggttcttt ttcaactaat gggtcaatga acttaattgg tttggcgtct
     5401 aaaatctctc cggttttttt atcatataaa tatccttcac ctttaccttc aataatattt
     5461 aagtccaatt ttaaaagatt atcatattca ttggggcttg caaagcattt tttaatagca
     5521 aattttgtca cgtttccttg taatccttta gctaattgtt tgtttacata acaattttct
     5581 tttttaaaat tgagaatttt cagcagatct gtttgtgtca gtttttcatt attatcaagg
     5641 tgttcgaaaa gtgcccattt ttgttcagaa gttaatacaa catcaccgcc cgtacgtgaa
     5701 ttgattttaa tattgtttat gttttcccac atttttgcca attgaaaaag cggagaactt
     5761 ttgggcgcaa catttggccc gacaaaatat tcttttccct ctttttttgt ccaaaagcct
     5821 tcaaaatcac agacagaaac caatcccttt tgagatttta agggacgttg atagtaaata
     5881 acttcatttc tgattttatc aataagctcc tgagacaagt agggctgatg tttacaaata
     5941 gtatcaaact cctcaatatt agcttcccgc gggaaaatat tctcttttat tctgaaatat
     6001 tcatcttttg tcagttcatt ataaaaatac tggccaattg tgagattcag ttctttaatc
     6061 ttatcgtgtc tgctctttac cgttgcaaca tattctgtat cttttttatc cagattggca
     6121 tcgctacggc tgcttttata tccgcgtttt tggtttaacc ataaaagaat acggcctagt
     6181 tcttttgggg aaatttcaga ggcagctgct ttatctcgaa gtgcccatag ttgttttttt
     6241 ggaagtgctt ttaatgcatc atcaggcatc atattatttt ttatcaaaat atcaacaaga
     6301 tattttcgac gtaattgata tctgtcatag ccttttcgag cggttcttgc ttgtgttcgt
     6361 aatgagtttc tgctttcacc agttcctttc gcaaaatctt tgccttcagt tccttcataa
     6421 ggaataatcc tgcaacctaa ccctaatatt tcacaagcag ttttgctatt ttcttcattc
     6481 tcattaataa ctgcccatcc aatagatgca gaacccaaat ccaatcctaa aatctttttc
     6541 atatcctaaa atttttttaa aattattaaa aatatttgca cgaatcgaaa tattttttat
     6601 ttttgcttct gaattagaag caaatcacaa taaggattat tccgttgtga aaacatgtaa
     6661 ggcggcgcaa gtcgcctttt ttagaattaa atagtaaggc tttttgctga tatcctacta
     6721 gatttttatt aggtaatcat ggtaaaaaag aggctgcctt gtttattcag gcagcctctt
     6781 taccaaaaat caaatttata aaaaatcaat gaattaattt caagatcacg catttagatt
     6841 tattcaggcc agtgtattcc agtttaaatg tgtacatacc tgccggcagg ttttttggtg
     6901 agaatacaac cgaatgtttt ccgctaagct gtgattcatt cacaggttcg gcaatcattt
     6961 cgccaatagt attatatact ttcagtctta cagtcccatt ttccggaatg ctatatgtca
     7021 aagtagcatc attgctgaac gggttgggat aacctgcaaa ctcaaatcct gcttccatat
     7081 cgttactctg gacaaatatt tttggcatcg tgaggtaggc atctgcattt atttcgccat
     7141 attgattagc tatcaaccct gtaccgttca gcttaaaatc tagttctcca agacctggat
     7201 cattgagcga aaggaagcct aatctgatca gttcatcatt tgccgcaata tcaaaataat
     7261 cagttgtcgc atatactact ctgattactc catctacatc tgtcacgagc aggtcattat
     7321 tagcagcaat gatctcctcc aatgcaggat taatctttat tgcaccacct tttttaccgg
     7381 tatttgacat ggacgtgctt acaagtttgt aatgttcact tggataactc agctccaggc
     7441 ccaatgcgga aagttcattc atttttgtgc ttattcgtaa aggaacttca aaatattcag
     7501 gatcactcat tatgatggat tcgtctgagc ggatgatgat attttcatca ggtaatactt
     7561 ttgccatacc ccagtttgtt aatgaatctt tgtatctgga actggatgca tcgtaatctg
     7621 cataggcgat cgtttgcagg gtgatgttta agtcggcacc ggccacggtc acggatgtgt
     7681 caagggctac ccagtttcct ttcgggaagt ttctggtgcg atcgtttggc aaaccgatct
     7741 ttgcactaat tctcgcaatg tcaacagtat taatagaggc attattatca acgtttgcgg
     7801 ctctcttatg tttaatagta aaacttctgg acggatcaga cactgtatcg tggccaataa
     7861 gatattttat taaggctaag tcaactgcat ttacatgatt cacgtattgc atggtatcat
     7921 aagggatgtg atcgtaagct aatatgtagt ttccatccgt gacattggta aattgaaaat
     7981 acccgttagc atcactcata gtggttgcca atacggtgtt tccactcttc agtttcactt
     8041 ctacattatc aatattgtaa attgcagcat tgtaggacgg catattgggc gcagggtttc
     8101 ctgcgtttgc cttggctgca taacgcgttt taccactgat ggtatggttg attcctataa
     8161 cggtcactgt aactgtaaag cttgcaccaa cgcataatcc tgaagtgggt gtcacagtat
     8221 aaattgccgt tccacttcct gaggggattg ttaacattcc actgatatct gtttgtggaa
     8281 tggcctgcgc gcttcctcct gtcacgcccc cggtataagt agggggtgtc catgtatagg
     8341 tagtccctgc aggaactcca gccgggataa ctgtgaatgt tgcacccgat gaaattgatg
     8401 ttgattgatc agcaataact ggtgtttccg gctgagtatt gatgaccaca ttagcagaag
     8461 ccacagaagt acacgtgcca tcatttactg taaagttata ggttccggca gcaaggcctg
     8521 aaatagttgt tgatgttcct gtgccgctaa tattgcctgg atttattgtc cagttacctg
     8581 ccggtaaccc gtttaataaa acacttcctg ttgatagcgt gcaggtgggt tgcgtgattg
     8641 ttcccactac cggggccgat ggtataaccg gctggacatt gatgaccaca ttagtagaag
     8701 ttactgaagt acatcctgac gagtttgtaa ccataaagtt gtatgttcct ggcgctaatc
     8761 cggtgattgt agtacttgca gtgttacctg taattgtacc aggattaatg acccagttcc
     8821 ctgagggtaa tccgcttaat agaacacttc ccgttgctaa atcacagttt ggttgtgtaa
     8881 tggtcgccac tgttggagca gccggtgtta ttggttgagc attgatgacc acgtttgctg
     8941 atacagcaga agtacatcct gctgaatttg tgacagtaaa gttgtaggtt cctggagcaa
     9001 gtcctgaaac agttgttgag gttcctgttc cggtaatatt gcctggattt attgtccagt
     9061 tgcctgccgg taaattgttt aaaaccacac ttccggttgc taacaaacaa gttggctgtg
     9121 taaacgttcc tatagtcggc gcgccaggtg taagcggttg tgcattgatg accacatttg
     9181 ctgacgcgga tgaaatacac gtcccgttat ttactgtata ggtataggtt ccggcggcaa
     9241 gacctgaaat agttgttgtt gttcctgatc cggcaatatt gccaggattt aatgtccagt
     9301 tacctgccgg taatccgctt aataaaacac ttccggttgc caggacgcag gtgggttggg
     9361 taatagtctc cactaccggt gctgttggta taagcggctg agcattaatg accacattag
     9421 ctgaggccac cgaaatacat cctgccgaat tggtaactgt gtagctatat gttcctgcaa
     9481 ttaaacctga aattgttgta ctggttgtcg atccagtgat agctcccggg tctatagtcc
     9541 agttgcctgc cggtaagccg gttaatatga cacttcctgt tgctaaggta caactgggtt
     9601 gcgttatagt ccccaatgtc ggagctgctg gtgtaccagg ctgagtgttg atgaccacat
     9661 tagctgaagc tattgaagta cacgtgccat cattcaccga aaagttatag gttccggtag
     9721 caaggcttga aattgttgtt gatgttcctg tgccggtaat attgcctgga tttattgtcc
     9781 agtttcctgc cggtaacccg cttaatataa cacttccggt tgctgccgta catgtcggtt
     9841 gtgtgattgt tccaacaacc ggtgtggccg ggattgttaa cggtgcggct aaaccagatc
     9901 ctgccggact tgtacacccg cttatcgttt gtgttactgt ataagatcca gttgaagaaa
     9961 catttatgga tgctgtagtt tccgctgtgt tccacaataa tgtacccgta aaagccgaag
    10021 ctgtcaaagt agaactaccg caattatcaa tcacgttaac ggtaggggca actggtattg
    10081 gaatttctgt aattacagca cttccagaca tggctgacga gccagaacca ttagtaccta
    10141 caacggtgta tgttccggcg acctgtaaac caaaatcaag ggctgctcct ataccgtcca
    10201 aagtcggtat ctgggcaata ttatctttat acagtgtata agtaacaccg atttctgaat
    10261 tagccagtcc gacagctaat ccgccggcac cgaaacaata agaaccactg cctgtaacag
    10321 cataaactat tggtaaattc tgattaagga caaggttatt aagattgttt gttgtttgtg
    10381 aagcaggaac cataatcgcg gtattcactc ctcctacata agcatttatt tgtgtgggat
    10441 aaatgtttgt ggtagtccat gtcagatttg gggaaaattg tccgaaggcc acagtatttg
    10501 tcaatcttac cctgcaaacc tttgtcccgg gcgatccatt cgaaatcacc gcgccgtttc
    10561 ctgctcccgg cggcaccctg ccggctattc taatacaatt tgaagccgcc agaaaattga
    10621 tacttgtcgg gttctggctt gctgctatca gcgcaggatc ggaactacct gcaacaatag
    10681 acgctgaaat aacacctcca ttttttataa gtgggttgat caatattcca tactggcctg
    10741 cagcatattc aaagggaata gatcctgtct gaagcaggta aatatcgaat tcaaagactg
    10801 tgctggagac ctgagtgtcg ttgcgtaatt cgcaaagata ggttggattc tgagcctgag
    10861 tgcccattga gatatggata attaatattg tccataataa gctgaaaaag tttttcattt
    10921 tcataatttt attttttttt gtgcatttat tgatggtttt gactttattt tagggcaaga
    10981 tagcagaaac gaaatttgcc gcgttgttat caataatggc catatcggct actccgacaa
    11041 aaccgtcacc atcaatgtct tccggtaaat atccacttcc aaacgctgca gactggttat
    11101 cagcagaagc catatcgctt acaccaataa atccgtcctg atcaatatct cccccgtata
    11161 ttgcataact gagatcaggc atctgaatca ggtttccacc aaatacatca gttggcgtac
    11221 cataagattg gttgatcgta tcactggcaa acgaaatagc tgttgccgtc gttgtctcta
    11281 aactattgcg atgccggatg gtgatgtaat atgagctgtt aaatgttccc ggaacaataa
    11341 ttgtcgctgt ccctgtagtg cttaagggaa catcaggtat tgttaacaca attgttccat
    11401 agacagcctc atcatgcaat tctaccgtaa tatgatcagc aattcctgcc ggccagtgcg
    11461 gccccagttc atcataagcc tgtagatttg tccccaggcc actatataat ccttccagca
    11521 taaccgagga aaggtttagt atcttgttac agctattgac ggtaactatt accggctgaa
    11581 tatcaaaaca gccggctgct ttggtgcctt ttatataata tgttcctgct gctgctgtgg
    11641 caggggtggc ataagcagat gttgcaccgg catcggtcca ataggtatat gtcaggccgg
    11701 ctgtgcttcc tgcggttact gccggagcag tgaggtcagc ggttccagga gaacaaactg
    11761 ctgctggatt ggtaataact aaagttgggg tgacacagac tatctcataa gcgccaatgg
    11821 taggattaac aacatctcgg atagtaccgg taatgtcggt ggtgatgcct gtaaccgtag
    11881 ttccagccct gagagagaaa ttcaaaggta aaaaatcagt tgcagaagtg aacaggggat
    11941 cagcattcaa tgaatttgca tctttacttg tcgctgtttg ccagtcagtt agtgttagtc
    12001 tgtctaatgt actatatcca aaatatcctc ccgttccgtt cgtataaata tcattataat
    12061 ttgaggttag taaacttgta cttgttgtat atatgccata atgctttcct gtaccggtac
    12121 tgtttgatct cgcattgaat actatgttat taagaatatt tgtagtgcct gttccccctc
    12181 tgttgattcc atagctatta cccgcggtta tgttggcgcc tcctatataa atggtattat
    12241 agtatatgtt tacggcaccc aaaccaatag tttgaatacc ataccaggtt gcggcaggat
    12301 gtgtaaaggc gatatcggga taaataatat tgttataaat attggttgtg gttcctgttc
    12361 cgccgctggt ataaatagca ggaatgcttc cggcactcat agagttaagc aaaatgtcgt
    12421 gtattttatt gttgaataca tttgtgattc ccgatacgct ggaaccaata ctaatacact
    12481 tgaaattatt agacaaactg gttgttaggc tgttgtatat gtcgttgtcc gatatttcgg
    12541 tatttgaaaa cttgcccgaa atatttaatg caagatatac aaagttcgaa atggtattac
    12601 ccgtaataac attatggtct cccgcaaggg ttgcagaacc cagcaacccg ataccgttac
    12661 ctgcaatatt tgcgttattg accgggttgg ttatattaca gtgactaatc gtattattgc
    12721 ttgatgcatt ggtagcattt gtaattgata tagcgtaatt agaagcacca tatccattga
    12781 tgctaaggtt ttttattgaa ttattagaag cgccttttaa gtcaattact gaacctacca
    12841 gatattgtcc tgtttgcacg atagccagcg aatttgttcc atcatttaat ccatcaatgg
    12901 tcaggccggt tacaccaata aagcttagca ggttggcggt acctccatta aaggttgcgc
    12961 ccgaaatggt tctgtttccg acgggattta ttgtcagggt ataaggtcct cctgcgccgt
    13021 taacccaggc attaagccct ttggcgcctg tttcggaaga aagatcgctt atgatattta
    13081 cggtaaggtt cccatctaac tgacccgcat tgatttggtc gaacagtccg ccagcattgg
    13141 ttaatgatgc aaaatcgcca cctgtgccaa cggtttttat tcccgaatag gcaccaccta
    13201 ttatgtaata tggaacacca gtaggaggag tgataattgt atttacatct gtagcaacta
    13261 ctcccaccgg atttgatttt atgtttggac tacctttata gcctgcctga tcctgagcta
    13321 ctacatagta gtaaatctga tcgcctgttg ttactccgcc aaccagcgaa tggtctacgg
    13381 tgaaggtcca tgtgccagtg gttccgttgc ctgcagatag cactccgggt gttgaaacat
    13441 aggtgccgct tacatttttc ttgtaataga tacgaggtac aaaacttccg gtggtgggta
    13501 tgcccgttac gggaacacct gcctgatcag ctattaccgt tgttaatata cggtttgcag
    13561 ttccgggggt gtttggaata ctgatgtttg tattgttatt gatggttggc gcggtattgt
    13621 cagtgccgaa ttgatatctt ttaacactag cattacccgg agtcatcgta ttactattgt
    13681 ttgcagaaac tccgtagctt gctgttgcac tactatttcc tgttacggtt accgtggccc
    13741 aatcggttgc tgaaactccg attcccatgg tataaccgcc agggtttgct gcgggataag
    13801 ttggaccaaa agcaaaatga acattcccgg tggcttcgta taattgtgcc tgaataacag
    13861 cattggccgc gcctgctacg ttttttggaa ctgttacatt ccagtttaca taaagtaccc
    13921 ggctgggtgc tgttccacca aggtagtagg atacacttcc attggtaccg gtagccagat
    13981 catcccaata gggtgcaatt ttagggagat tttgtgtcga tgctatactg tttacaggtt
    14041 cgggaactat aacggtaccg cccaacttca tcaatccgtt ttcatcaacg gaaaactgag
    14101 tgtaagctgt tcctgcaaac cagaaagtaa aaccaatatt ggtgactgcc gatgcaacat
    14161 tatcagaatt agggccaatt aactgggtcg gggttatacc ggttggacct gtgttggccg
    14221 cgttaaaacg ataagtgtta acattgattt gctgtgcaaa cacgctccct gtcaaaaaca
    14281 gggtaattgc aaataagaaa agaaatgttc ttttcattgt ttgagttttt ttcatgagtt
    14341 ctatttttat ggttgaattt ttatttgata attagtgttt tctgataatt cttactaccg
    14401 cttagtacat ttattatata acaccccggc gtttctattc tgtaatgagt agttccgata
    14461 cctgtctgtt catcattaat gattttctga ccgagcatgt tataaataaa tataaaggca
    14521 tttccctgtg tggagttgct gtaatttaca tttatgtatt tgcccgaaga ccatgcatca
    14581 acaaaagaat tgctgttttc ttctatacca gtaacaatgg taggtgagaa cctgatgaaa
    14641 aaccggaaaa gaggagcgtt tgcattgatg gtgaatgtgt attcgggact aacagttaaa
    14701 tcctggcttg ttgaattttg gttatcttca aggtatatat ggaacgacgg atcaaagttg
    14761 agaatttctg ttgctttgat tgtgaaactt ccgcttgtct tagttgtaaa gccaagaggt
    14821 atcaccacat cggtgtttga aagagacggt aatgcactaa tagataaaga ataattagaa
    14881 ctgtcctttg taaatatatt ggggtaagcc ggatcgttgt tcagcagttt ataggcatcc
    14941 atgtttccgt cgaatataaa agttgccagc ggatcaaaat aaactactgt ttcatcagca
    15001 tgaagttcat tcatggcggg atagccttta agccttaata gtgcatcagg attttctaag
    15061 gttttattaa acgacggggt taaatctgtt gtccttacag catcgttaac gcccagtatg
    15121 ccattggcgg tggcttttac aaagaacccc tgcatggacg gaattatccc ggtggttccg
    15181 gacggtatgc caatgccatt gacaaacgaa ctgtatgtcc cggtatattg ggtattcgga
    15241 ttgaaatagt aaatgccatc actgacattt atttttgtcc agccacttga agcctgccag
    15301 tcaattggag agggataagg gttgcctaca aggttccatc cgtcggcata gttattacct
    15361 gttgttgatt ttgttacgct gaaatttaat gctccgctgt ttaccactcc cgttacatcg
    15421 gcggtgagag gcgctgtaga tgcgccgaaa ttaatacaat agccccgcat tggtgtcatt
    15481 gttgtgccgg cacctgtcca tccgatagac atggagggcc ccagaggctc atcatacatg
    15541 aaaaaatttg gaaagggcgt caccgtatgt gtggtatcat taccaacata aggattgcct
    15601 gtaataagcg ttcccagttc gtcagtgaat tcattgatag gcgctccggt gaacggtgaa
    15661 gagtaataat gatatccaag cctgttttcc atgtagcgct ccatcgtaac attgcctgtt
    15721 acactgccgg agcctgtacc tgctatcaat gcagttcggg aagctgtcga cagcaatgtc
    15781 aggtgcccgt tactggctaa tacgccactg gatggtgtta aaatacctgt cagttgagtg
    15841 ttgttattta ttgttacccc gtttaaatta cttatggtaa gattaccaaa agtggtgact
    15901 ccgtttattg attgcgtcaa tccgcccaaa aagatgactg ttccatctcc tatgttgctt
    15961 tgtccgttat ttgtccagtt gccataaact gataagtcac taccgctgtt aacggacaat
    16021 gaggccccag aatgaatggt aatactgtgg cacctggcag ttcctgctga gtttacaata
    16081 ggtgattgag ctgcagatgt gataataacg tcggtagagc ttgttggcac tgcaccgcat
    16141 aaccagttgc cgggattacc ccagtctgaa tttacaactc ctgaccagat acccgccggt
    16201 tcaacgttgt ttagattatt cgttgtttgc gaagcagtta acatgatggt tgtgttcgaa
    16261 cctcccacat aagcatttat ttgtgtggga taaatggcct gggtggtcca tgtcagattc
    16321 ggtgtaaatt gtccgaaagc aacagtattt gtcaatctta cccgacaaat cttggttccg
    16381 ggtgatccat ttgaaatgat agccccattt ccggaacccg gtggtatccg gccagctatt
    16441 cttatgcagt ttgaagcatt cagaaaactg atatttgtcg gattctggtt tgtcaatatt
    16501 aacgcaggat cggaactccc ggccacaatg gacgctgtaa tcacacctcc gtttttgaaa
    16561 aggggattta ctaatattcc gtattggcct gtagcatatt caaaaggaat tgttcctgtc
    16621 cggatcaaat aaatatcaaa ctcgaagatg ttgcaggaga cctgcgcatc gttgcgtaac
    16681 tcgcaaagat aagttggatt ctgagcctgt atgctcactg aaatatggat aataaccatt
    16741 gtccatagga aaccaaaaaa gtattttatt ttcatttttt tgttttattg aataaaatta
    16801 tttttatcga tttttttcaa ttgtacaaat ttatgatgtg gcgaagcgac tacaattaaa
    16861 tgcaaattaa atgcgcatta agaagtgatt aaccggaaaa gtagtgtgat aaggatggct
    16921 gtgaaaaatg tgaagattat ttttcagtaa ttatttctat tcagaaaaaa aagttcatta
    16981 taaattgttt gtcaattatt aaagggcatc tctaataacc ttgatcgggc gcgtctaaaa
    17041 accaataaag ttcgaggcag ttgaagttct gaaaaccgca atgtactgag cgtacatgcc
    17101 tgtgctgagc ctgccgaacg caaggatttg aagaacgaag acgaacgaag aaatttgaag
    17161 tttttagagg tgcccttaat atagggcggt tatgctaagt acaataacct gagaaataaa
    17221 taaagacagg gttaaaaaca ggtggatccg ggaattacct tattcccgct atcgggagct
    17281 atttagcatc tccaaatatc tcatcagaat ttatcagcaa atcaacatac tgggctcttt
    17341 tataaataaa aggattactg ctgagtttgt ttttggacaa tttgcctctg aattcatcaa
    17401 tagtattata gtttttaact tccatccact ctactaattc attctggata gtttttattt
    17461 gggtcaggcc atttttgtat aaagtactca ccacttgcgc acaggttgcc ccggataaca
    17521 gcagctttat aagatcttct cctgtatata tcccatggct gctgcaaaca tcagcattga
    17581 tattttcgaa taataatccc gcatatctca atgatttctt gtaatctcct tcattactga
    17641 gattaaacga tttgatgtgc ttctcacttt ttatatcaat atcgggttga aaaaaagaat
    17701 taaacaaatc aaatgctttt actccggtct ggtcaagctt ttttataaaa ctcagaatat
    17761 tcgtgtagtc agaacttaat ttgacactta ccggtattga aatattttgt atgatatctt
    17821 ttacaatacc gatctgctcg tcttcaattt cctgagcatc ctttgtaaaa tctgtaggat
    17881 tctggtagaa attcagttcg ataccatcaa ctcctgtctc gcttaataat ctggcatatt
    17941 tgacccaggt tacattattg acggcattaa ggctggctat taccgggata aataaacttt
    18001 ccttggcttt tctgatattc cacaaatgtt catcaggacc tgcatgttcc aatttcggat
    18061 gtgttgtaat catttcggca ttgagatcgt tgaattctgt aagcttttca tccatctgaa
    18121 aattctcaag ttgtatctgt tcctcgaata atgatttgta aacgatagct gcagcgccaa
    18181 gttcttcagc ctttttaaga ttatctaaat ttgctgttag actagaagct cctacaatga
    18241 tgggggtttc caattcgatc cccatgtact ttgttttcag atttatcatt ggaatgtttt
    18301 ttaatttatt agttactcta attttttttt atcaaattat catcctgcca ataattgggg
    18361 gtacatataa atgtaggttc ccatcagtat tgatatatgg agaacatgat caatactaat
    18421 cacggcaata tattttgcct catttttctg ccaatacagt tttttaaatt tgctggtaaa
    18481 aaaatctacg atcaggtgtg caccaccgtt tatagcacta aaaaccaatc cttgcacaaa
    18541 agtcagttcg agtaataaag ggctgaaaac aataaaaaat atcgtgtaaa tccctacatg
    18601 ggctagtaaa aatccggttt ttgatgcttt taatttatat agtctgttgc cttgcaatat
    18661 aaaatctgca gcaatgtgca tcatgattaa aattagtatc ctgtacattt tgtttaaatt
    18721 tttggtttgt tataaatcat ttatacaaat gtacgcgcat gcattccttt tggaattaaa
    18781 tgcaaattaa gattcgatta agatttgatt aaaatatgaa tttctgcaac aaaatgcagc
    18841 atattctata aatatctttt ggttgtatta atgctgaatt tcttcttctc tcaacacata
    18901 accaaacccg acaattgtat gtatgagttt tggagagaag tccttatcaa ttttgtttct
    18961 gagtgaattg atatgaacat caattaaatt tgtgcctgaa aacgaaaagc cccatatttt
    19021 ttctgcaatt tccatccttt tgaacacctt gccgggactg cttattaaat attccagaat
    19081 cgcaaattct tttgcagtaa gtttaatttc ttttccattt ctactcactt tttttgaaat
    19141 gctatccaat tccaaatctg caattttttg aataatgggt attactacag catcagtatg
    19201 tcttctgttt aatgccttta ttcgcgcgag aagttctttg aaatggaaag gttttaccag
    19261 gtaatcatca gcgccgcaat cgaagccggt aattttatct tccaccgaat ctaaagaagt
    19321 aagcattaaa acaggttttt taatattggc tgtcctgatt tttttgcaca attcatagcc
    19381 atttattccg ggtatgataa cgtctaaaat gataatatca aattctttac taaatacgat
    19441 tttttcagcc atcgcgctgt cgtatgcaac cgatacatcg taaccggaat cctcaaggcc
    19501 aagttttaca aaggaagaaa ttttggtgtc gtcttcaact aaaagaattt tcataagagg
    19561 ttataaattt acaacaatgt attttagtat tttaaataca cacaatattc ataacaaata
    19621 tagtaagaaa agattaattc gtgataaaaa aaagatatat ttgtaattcc aagcgatatt
    19681 tataaaacta aaggggaata ttcaattcct gtaaaattga aatgatacta tgtagtgcat
    19741 attttgtaac acaataaaaa taattatgag aaagttaaaa attaaaggtc tctatcaatt
    19801 atttgttatt ttattaatga ttacattatt tctgacggtt acctctattg tcattattta
    19861 caaaatagtt tatcaggaaa aacgagttta tttaagagaa ttgtgtgaaa gtcgtaagga
    19921 atttattgag tcaatttatc agcaaacaaa caatccggct tttgttttgg aagttttata
    19981 taaacagcaa agagcaaatg atggtcttgg tatttccggc gaatttatca ttggtatcga
    20041 aagaaatgat tcgatccaat ttttatacaa aagcaagcag aaagtcgaag caaattatac
    20101 ggttatacct aaaacgtctg acaaaggtat tccgctgcaa tacgcattgt caaaaaaaac
    20161 agggtttata caaggaatag attatcaagg agtcctggta ttatcctatt gcacctatat
    20221 tccggaactg aaatggggag tggtcacaaa aatggctctt tccgaagtgc gtttgccatt
    20281 ttacagggct ggttttattg ccattattgc ttcgtttatt cttttcctga tcgcaaccat
    20341 tttttttaga aagatttcct tctcaattaa aagaaaaatt gatgatagcg aagaaaaatt
    20401 gactgccatt ctgaatgcct cacaagaatc catttttatg ttcgatcaga aaggtactat
    20461 tattattgca aatgatattg cttcccaaag attaaaaaag accctgccgg aaattatcgg
    20521 aaaacatttc tctgtcttta ttcctgagga aatcgttcat gaacgttggg catacctcac
    20581 caaagtgttt gattccggaa aaccatatca atttgaagcc tatcgtgatg atgctatttt
    20641 tgaacataac ctgtacccag ttttcgaaaa taaaaaggtg gcctatgttg tcagctttag
    20701 ccaggatatt acagagcgaa aggggatggt ggagcaaaaa gaatttcatt caaaggtgat
    20761 ggaaaccatg catgatgctg ttattgctac agatacgtca tttataatca cagtatggaa
    20821 taatgcagct gaaaaattgt atggatggag caggaaagaa gtattaaata aaaactctcg
    20881 ggatattctt aaaagtacta ttagcgatga tgacttaaaa aaaatgttat ccgaactcaa
    20941 aactgataat aatgctcatt atgaaactat acaacacaga aaggatggga cgcccattac
    21001 catcgaagcc agattatctg ttataagaga tgaaaagggc gagattgtaa gttatattgc
    21061 tgctaacaga gacatttcgg aacgcaaggc cgctgaagca gcccttcaaa aaagcgaagc
    21121 aagccttaag agatctcagg aaatggctca tcttggcagt tgggatctgg atattaaaac
    21181 aaacattgtt atatggtctg atgaagtgta tcgtattttt gggatgaaac cacaggaatc
    21241 agatattact tacgaaattt tcctgagcag gattcacccc gatgacaggg actccgtggt
    21301 caaagcttat tcgggatcta ttagtgaggc tagggacacc tatgaggtgg atcatcggat
    21361 aataaaaaaa aatagtggtg aaatccgttt tgttcatgaa aaatgctcac atattagaga
    21421 cgattcaggt tttatcatcc gttcagttgg aatggtgcat gatatcaccg aaagagtgat
    21481 gtacgaagaa gcattaaagc aaagtgaaga aagcgtgagg ttgaaacttc agagcattat
    21541 gtcgccagaa ggaaatattg aaaaacttga actggaagat attattgata cgcaggcgat
    21601 acaatccctg atgaataatt tgcatgtatt ggtcaatatc cctgtggcca tcattgacct
    21661 gaaaggaaaa gtgctcgtag gagtgggctg gcaggaaata tgtacaaaat ttcacagggt
    21721 tcatccggag acttgcaaaa actgcatcga gagtgatgtt cagcttacgc gcgggacacc
    21781 cgaaggtgaa tataaattgt acaaatgcaa aaataacatg tgggacatcg caacacctat
    21841 tattattggt ggagaacaaa aggggaatct ttttatgggc cagtttttct ttgatgacga
    21901 acaggtggac cacgcctact ttgtggcgaa ggcgaaaaaa ttcggctttg atgaaaaaga
    21961 atatatatcc gctcttgaaa aagttcccag gctttcaagg caaaagctgg aatacgcaaa
    22021 atcttttttc cgggaatttg cccattcact ttcacaatta agttacagta atattaaact
    22081 tgcgcggtca atcagcgaac aggataagat cgcaaagaaa ctgaacgata caaataatta
    22141 tcttgaagcc cttatcaatt atgcaaatgt gccgattatt gtttggaatc ctcaatttga
    22201 aattcaggtg ttcaataaat cattcgagca tttgtcaggc tttttatctg aagaagttat
    22261 cgggacaaaa ctcgattttt tatttcctcc agaatccctc acagattcga tggaaaagat
    22321 aatatctgcc tcattcgaaa agttggagtc tgttgaaatt ctaatccagt gcaaagacaa
    22381 agaagcaaaa actgtgttat ggaattctgc caatatttat gatacggata acaaaacttt
    22441 aatatcgatt attgcccagg gcgttgatgt aaccgaacgt aaaagaatag aaacggcatt
    22501 aaaggagagt gaagaaaatt accatagatt atttgagttt tcagcgatac ccatttggaa
    22561 agaggattat tcagaaataa aaaaacactt tgaggcactt aaaatttcgg gtgttacgga
    22621 tttcagaaag tattttgaaa cccacatgga cgaggtaatt catttggcat ctttgataaa
    22681 agttgtggaa ataaatcata aaagtcttga attttatgga gccgagagca aagaagatgt
    22741 aattaaaaat atgcttttct attttaatga agaatctctg aatgttttca gagaagaatt
    22801 aattgcgctt gcttcaggtg aaaagaaatt cgaatgtgaa atgccaatga ggacattgtc
    22861 cggaaatgta atgatattag atttgcatct atcagtcgta aaagggttcg aagatacctt
    22921 gtcaaatatt ttagtttcat ttattgatat taccgaaaga aaacgatttg aaaatgagtt
    22981 acaagaaaaa aatgaggagc ttaaagaaac caacgccact aaagacaagt tttttaatat
    23041 cattgcccac gacatgaaaa atccgtatat cagcttactt ggggcttctg agttgctata
    23101 tgaaaatgca aaaaagtacg atagagataa gattattacc cttgctaaaa ttctgaacga
    23161 ctcggcaaaa agtggttatg atatgttgtt aaatatgctt gaatgggcac gatcacaggc
    23221 agggagcatg atatttcagc cggaaaaaat taacttaaag gcgttaattg ataagggcct
    23281 gtctaattta attgaatatg caggaagtaa aaaggtaaat ttaaaatttg acattccgga
    23341 cagttttcaa gtatatgctg acaagaacat gctaacttct attttgcgaa accttattac
    23401 taatgcattg aaatttacgc cagaaggcgg agatgttatt gtgtggacca aagaagaaaa
    23461 caggtcgatc atagttttta ttaaagatac aggagttggg attgagaaag ctgacctaag
    23521 caaacttttc agaagtgata taaaatatag tcaccccggc actaaccatg aaggaggcac
    23581 aggtttagga ttactgttgt gcaaagaatt tgtagaaaaa catgggggga aaatatgggt
    23641 agaaagtcag gttcgcaaag gttccgtttt ctatttttca attcctgaaa aaatcgtatg
    23701 attttaaaaa aaaagcttgt ggatcaaagt ggtcataagc tacattttac atagggctca
    23761 atttttttga aatcaaactt tatcaaaatt cactattatt gcttttttct aagaaacttt
    23821 tttgatttta tgttacataa tatttatgat ggatttttta gcgaaacaag gcagattttg
    23881 ctgacatagt ttatcctatg tcgaaaaatc taacgaagtt gcagcaaaaa agacgcataa
    23941 agatattaaa agataaaatc aaaacaattt ctaattatta aatctttttt ctatcaccgt
    24001 ccagtcgatt atagaccaaa aagaattaat atattcattg cgtctgtttt gatagtcgag
    24061 ataataggca tgttcccaaa catcgcaagt aagtataggt tttaatccct ttcgaagtgg
    24121 atttccggca ttaatttcct gaatgatttc cagctgtccg ttctggtttt taactaacca
    24181 aacccaccca caaccaaata ggctcactcc tgcttttgta aaagcatctt taaaatccaa
    24241 aaaagttcca aaaccccttt ggatagcatc ggcaagaatc ccatgcggca aagacttatt
    24301 gtttctattc aatgccataa aatagaatgt atggttccag gcctgtgctg cattattcaa
    24361 aagaggccct tccgcctgca tgataatagt ctcaagtttg gcattctcaa atttggtttc
    24421 agaaattaaa ttgttaaggt tagaaatata agtctgatga tgttttccat aatgatactc
    24481 aagagttttt cttgagataa atggttcaag ttcatcaagt tcataaggta atacaggcag
    24541 ttcaaatttc atgattcaac tgttttattt attaaacagt caaaaatagt ctctaaatga
    24601 ctaaaagaaa ttaaaacacg gttaagatta gattaataaa ctattaagac tattacatta
    24661 taatgaatta aggttattac atttctccaa ttagtattac ttttccacaa caaatctttt
    24721 tcttaaaaca ctattgtcat taaattgtaa ttctatcacg taaactccat ttacaattcc
    24781 aggcaattcg atacggatac tagtaacgtc cttaactatc gttttgcaat ggataatacg
    24841 tccgggcata tccgtgatgc ggatctcttt cagactggag aagatatctg actccccggg
    24901 aaactgaata taaaaacttc cttgattagg attcgggaac agggtaaata ttttattctg
    24961 agaggcttcc tctaacccta catgataact atattcatcc gaaaaggcag agcagccgtt
    25021 agcaaaacct accagcacca tatatgagcc ttcggcagct atagtgatca tcgtatcgtt
    25081 ggcaccgggt ataatgcttc cctgatagta ccactgatac tgtgtgccct gtgctgaagt
    25141 atacagtgtc agtccgttca ggaaaaccgg cacatgcggg actgtatcca cgtgtatctg
    25201 aacattaccg gaaatgcaac tatgctcctg atcgttaacc aatgcacaat gattatttcc
    25261 accatcgaaa ctgacaacgc caaataagga agttatggcc tttggctcat tgacagcgga
    25321 ggtgtcctgc tgtccaagct gcccgttttt attgaatccc caggagaaaa cattatcatc
    25381 ggtacgtaaa acataagatg aatactgtcc ggccctgacc tgcctggcag atccggcttt
    25441 tattattttc accggataca aagcgtttat ggaagattgg ttgccgatct gacctttact
    25501 gttatccccc catgtatata catgacctgc cgtatctgct gctatagagt ggccaaagcc
    25561 gccatcgaaa acagctaccg gaaggggcag atttactttt acaggaatca tactgcttgt
    25621 attcgtccca ttacccaact gtcctgaatt gttggcaccc catacccaga gtgatccgtc
    25681 gtttttgaga gccatgctgt ggttgccccc tgcacggact ttcaccacat tatccagccc
    25741 gttcacttga acaggcaggt ttcttgtcaa taaagtgctg tcgccaagtt gtccaaaatg
    25801 gttgtagccc caggcccaaa ccttaccgtc agcagtaaca gcaagacagt gattgtctcc
    25861 ggcagcaatg tcggtaacat cgctgagccc gatgacagga aacgggaaat tgctggtgga
    25921 atagttgccg tagccgagtt gtccctgggt gttttcaccc cacgaaacaa gtgtgctgtc
    25981 ctgcagtaaa gctacgccga aacgtttccc cgcggcgata tgggtcgcat ttgtaatatt
    26041 accgacagca acaggcaaag gagaatcggt gtagttgcca ttgccaagag gcccgaattc
    26101 atttctgccc cacgatctga ctgtgccgtc atttgccagc gccagcgtga actcggcgcc
    26161 gcagacgact tccacgatac tgttcatccc ggaggcttcc ttaaaatcaa gggcattcgt
    26221 taatgtccct tcacccaatt gtccaaagta attggctccg gccgaaaaaa caacgccgtt
    26281 gcctcctgtg gctgactgca ccgcataaag tccttcagtg aatacatgta attccgggct
    26341 ggtttcacca gtaatcacag ctccatcgtg ataccatgtc aatccatctg ttacgctgct
    26401 ttcaaggatt gcagaatctc cttcacaaaa agtcggggaa ggcgtaactg ttatctgggg
    26461 aactgaaaat acatgtacca gaatgttttc aaaagcattt tgtaaacaac ctgttccgag
    26521 tgaggtgcct tccacactga ccacctggcc gtttatcagc gaggcagaag taaatgtgtt
    26581 acttgctgac gggctcccct gagacacacc atcaatagaa aactcataca gattggcgcc
    26641 gttggcagta aatgtcagcg gatcgcccgc acagatgaca ccggatggct gagaacatcc
    26701 aagggtaata ttaatataat tgatatccac gatgatagtg gtatcggcat ctgtccagca
    26761 attaccgttt ccgcctctca tagcgaccac ctggccatct tcgagtgcgt ccgtcataaa
    26821 cacagaatcg taagataccg gaatgccatc gacaaagaaa atatagttct gtgctccact
    26881 gccattaaaa ataacggtat cgccgtaaca gaaagcaggc ccgccggggg tagctgaaag
    26941 cactgccgat ggataattat ttaccgtaac tgtataatca ttatttccgg gagaaaagca
    27001 tcccgattga tatccggcca cagatatcgt ttgtccgttg gtcagcgaat tggaaataaa
    27061 catattgttg gcagcaggcg ctccggcaga tatgccttca acaaagaact catacgtagt
    27121 tgcccctccg gccaacagcc tgagcgtatc gccactgcaa taactgagcg gtgttaatgc
    27181 cgttaaagta actattggag taggcgtgac cacataggta tatatgtttg acgaggtgga
    27241 agtgcataat ccggtggtgc cgtttaccgt gatgatagcc ccgttgaata ttgctgatga
    27301 ggaaaatacc gaatcaggcc cggctgctcc ctgggatgtg ccgctgataa aaaattcata
    27361 agtatctgca ccactagcgg taaatgtgac catgtcgccc gaacatatgg tggtgtcggg
    27421 atcgctctgt gataaaacca ccatgggact gggattgacc gataccgtat agctgttcgg
    27481 agaggttcct gtacaaccct gagcagacat ggcctgcatt gttaccacct gcccattggt
    27541 gagggaatct gtagaatagg tgctgaaaaa actgaaggcg ccaacgctgt ccccgtcaac
    27601 gaagaattgg tatttatcgg cgccggaacc tgtaaaagtt attatctcgc ctgcacagat
    27661 cgttgtatcc tgatcagaac aggccatggg cgcaacaggt aaaggattaa ctgtaactgt
    27721 cacgggcgaa ggcaggcagg ctcctgcggt gccgctgaca gatacctgct gtccgttgct
    27781 caggcctgtt gctgtgtata ttgacccatc gacaagaaca gtatcccaaa ctccgttaat
    27841 ataaaaattg tacaatgaat caccgtgagc cgtaaactga acaacatcgc cggcgcagat
    27901 ttcattggcc gaaaacgtcc atgaaataga aggccgaaca tccataacga tactgtcagg
    27961 ctgggattgg caaatgccca gtgttccggt aacaaggacc acctgcccgt tagtgagcga
    28021 atcggtagaa aaattgtctg aatttgaata aggcccgata atgttcccat caacggaaaa
    28081 ctggtattgg tcggccccga aagcctggaa atttatgaga tcccctgaac agattatgtt
    28141 atctggatca gaactcgtta ggctgaccac gggtgtcggc attaccgtga actccagata
    28201 atcatcgctg aaagccatgc acccatcgga agaaataccg gcagccatta cattatcacc
    28261 attggaaagt gttgtcaatg tggtagtatc tatggacgaa aatatgccct gtgccatgcc
    28321 gtttaccgaa aactggtata agtttgcgcc ggaagccgtg aaaactaccg gagtgccgta
    28381 gcaaatggac agatcggcat cggaactgat aatagctaca tcgggcaggg gatgaaccgt
    28441 aaccgtcaat gtatccgtgc tgcctgcgct gcatcctgta gtagtatttt gcccatagac
    28501 ggtaaactgt tgcccgtttc ccggagcagg aatgatccac tggtttgatg tgctgaatgc
    28561 gccgtaagcc gtgtcgttcc tgtacaattg atatttgtcg gcaccgctcc ctgtgaaaat
    28621 gatagtgtcg gcagcgcaga tggtggtatc ggggtcattc gatgcaagat gtaccaccgg
    28681 aaggctgttt acggtataga taacgtgatt actggtatct gcgcatccct ggtaggtaag
    28741 aataacaaaa atttcatccc cgtcggccag gctggttgtg gcgaaaatgg aatcggggga
    28801 ttgctgtacc gtatctcctg cgacaatgaa ctcgtagaaa aggggagagg gattggagga
    28861 atatgcatta aaaaagacag aatcacccag gcatattgaa ttatcaattt ctgatgaatt
    28921 taaagttacc tcgggcttat atatggtgag cgtggtatct ttatatacgt caaaacatcc
    28981 tgtcgcggta tctgtggaaa tcaaagtaac aatatagctt gtattatttg cataaagatg
    29041 ggaaggctgg aatgtgaaag aagaatcatt atcaccaaat gaccagatga aatccatatc
    29101 tacagggttc ccgtgagtag ttgataagtt agtaaattgg atgctgtcgt taaaacaggt
    29161 agtggcagga agtgaaaagg aagaagtaac gtatttggaa gtgtcaaaaa cccatccgga
    29221 attgttgccg aggtctttac tgaataaggc tgaaactccg gcgggagtag ctgttatttt
    29281 ttttacattt agaaattttc ccgcgaagcc ggttgtgccg ttcttgtgca atgttgctga
    29341 tgcagtccct gtggtttgca gataaatgga gtcaaggcag gttccattga gtatgatgct
    29401 gtcaaatgcc accgtaagcc cggcttttaa ttccagatga attccgggat tgagcttcag
    29461 cagttcaaag gtatcagaac tcttgacaaa tgccttgtaa taaactccgg cagtgtcagg
    29521 cgcccttgat ctgaactcca atcctgcata gtcttgccct ccgccgtcaa acaggttata
    29581 atagtattgg ttatcatcca tcacaaggtg tgatgagtcc atgtttaaat tcaacaaaga
    29641 gccattttgc ataacgaaat tatatgcagc tgttatttgg ctgtggctga aatcaacatt
    29701 ttttatttta ttaccgacag tggtgataag gcctgcataa agatttttct tatttgatga
    29761 aaaggttcct gatagaaaag taacagtgct gagtgtatcc atgtaaaggt tttttagcag
    29821 gaataccgag tcggaaatat ttctcccctc aatattcagg ttaacattga attctttaca
    29881 gggcgtaaac cagcagtttg cattttgcgg tgataggata aagtggggcg cattaatatc
    29941 tgtaggttga aaattatttg tataatcaac gatcagggtg tcgcaaagcc tgactgagcc
    30001 tgtgatagtg aggtcgccat tgagcagcag ggcagggctg cggtttatgg ttgaatccca
    30061 ggtcatggtt ttacaatacg cattggtttc gacaataaca gtatcatgaa tacccggaac
    30121 tgaaaaatga atgctgtcaa acacaacagt gtccataggc ccgggaatac aaagtgcagt
    30181 agcattatta acttcccagt ttaaccggtc attccataat tttgattgta atccagtcca
    30241 ataataagta tttcctgaaa cagatccggt aatggtccac ccggaaaatt tattcagccc
    30301 aattgaattc agggcattat aaatatgacc ggtatcgctg actgcacata cattgctgat
    30361 gatcacatgc tcaacattga tgatatttat ggtgtcgttg ctgtttacca tgaataatgc
    30421 cgtatcgaca gacccggaat agttgccgat ggttattggg taatcacatg aacctgaagc
    30481 ctggatagaa tcgaatttta ctgttttaag gggttcacat tccaataatg ttcccggcct
    30541 tatcttaaaa aggcggcaat ccattccgcc ggctatccgg gcttttgggc aacgtatatc
    30601 gagtgtgtca aaatcgcggt tttctgcatc aaaaagcatg aaatccggtc ctgcaccaac
    30661 caggcgtatg gaggatgaat catgcaacaa ggccaggttg ctccctgcag atttccatgc
    30721 agtatctgac ccattaaggt atataacact gtttgaatag ttaagcgttc ttatttcatt
    30781 cccttctgac aaaaaagcat gacagcgcat atcattatta tcaaggttca gtgtcccacg
    30841 cacaaggtta attttccctt tcaaatttaa tgtatcactc aacctccatt cataggagct
    30901 gttaatttcg atgtcgccgt tgatatccgc gccattgctg gtgattgtcc tgtatccggt
    30961 attagggttc tgcagccggt accccccagc aagtttgaaa tgtgaataag aattaagaac
    31021 gatatttcct gtaacgaaca atgctccatt acctttgact gtatcatgat acgctgtgtt
    31081 gatccacatc agattttggc aataagcttt ttcgtcaact gtgatacttt cattagaacc
    31141 tgaaaaagat aaattgtcaa aaacgacatt cgttgactta gtggggatac atgaatgtgc
    31201 agttccacct gaaacagccg accattgatc cggatcattc caattgccgc taccgcctac
    31261 ccaatataag gtcggagggg ttgccggttc atttatggtc cagccggtat tatttccctc
    31321 atcgaatgaa ttatttgcaa caggatttcc cgaagctttg atattgctta attttaagta
    31381 gtctgtagta tcaggatagt tgaaatcgaa atttggaaga agcgtaccgc atccctgtac
    31441 attttcgcat tgtatacaag acggacaaaa tgcctttata taattataac ggccacaatc
    31501 tcctgcagaa gaaatactgt ctaatgtaat tgttatgctt gttgccagtg caatattgga
    31561 tcctgtgcca aatgccagca acgaggtgtt gaatgaatta aatatcattg catttgatga
    31621 aggcaggtgg atttccttaa agatgctaat gttattggtg tcttttcctg ttcttatgat
    31681 gacggtatcg ttgcctgaat aattgaaggc aatgatcgcg gaatctgctt taagagtcag
    31741 tccctgtgat tgaaaattca gggcgttgcc cggagcgttc aaggtgatca gggaagtgga
    31801 taaattaatc gtccgggtca aaggagtagc actggtaaaa gcagcgcaat tcaagggata
    31861 tcctttggaa taaaatttac cccctccagt aatttctatg ctgccggcaa cagacagacc
    31921 gctgcgtaag aacatgtttt tgctgccatt aaaaataact tttccgggga gagtgtttaa
    31981 atacaaattg aaatagtttg tatcaagaga attatatgca aaatatacag gcccgttttg
    32041 caggaagtca agtgagtccg ataatgccat agagcctctt atagtgagcg tatcgttact
    32101 gaaccatttg ggatttgcga caggaatgga gttttttaac gtggagtcgg tgttgaaatg
    32161 ctggcaggta acactatgac cgttggtgaa taaggatccg tcaacgagag ataattgatt
    32221 atagggcata tataacctgc tcagaagcct tactgaatcg gacgaaagaa cgatatcgca
    32281 cgccagcact ttgtttgcaa aatccaggtc ggatacctgg gcgggagggg acataaaaac
    32341 aagcactccg tagaaattga aatttaagtt gtttgaaagg atacaacttc catagatttt
    32401 taaggtgtct gaatttcctg aaaagaaata aatattaaat gaaattccgc tccagtccat
    32461 cattgcacat tcagccaccg gtatgtccag gttcaccgac tgtccgcttt gtgtaaacga
    32521 attagcgtca aaatacacgg tatcattggg tcccggaatt acggattgca tatttacagt
    32581 atcgccggaa gatttagacc aatgatggct tacatccgtc cagttacctc cgttattcac
    32641 ccaataataa ttatcggcaa gggctgattg ccatacggca aataaaaata aggtgagaga
    32701 taatattctt ttcatatagt atgattttga tcttaaaatg cttgataata aatgtaatcg
    32761 taaaaatatt tctggtaatt agctttttat ttcgcacaaa aaataattaa ctcaaattag
    32821 cttataaaaa gtaaaaaatc aattataagc tgttaaaatt acaaacaatt ttttgtttta
    32881 ttttaataaa aaaaaatttt tttaaattgt ttctttttaa tgattatttg agttatattt
    32941 gtagattaaa tcataaagat atttaattct tatatgttta gttcataaaa tcacaacaat
    33001 atactttaat attctagata tgaagaagca attttttata atttttacat tatgtttatc
    33061 ttttactacg gctttttgtc aggtacaaac aaccccaatc gatagtacat tttataaaca
    33121 ggtacaatcc ggttcaggca atagcacaga attcacgctg aagccaacca ccacttatga
    33181 cagcataggt atccctgtgg atgtgaacag ggatctcaca gacacactga ctcttctgcc
    33241 cggtacccat gcaaaactta agctgccccg ttattttgaa ccgttcacct acgaaaaatt
    33301 ctcggggttt cttcataaag gaacttcaac aagtattctg gccaatgaat atcagggaat
    33361 ggcatataca acaatgacat cgcagttaac ggactcaact ttttcgaaac agggtgccga
    33421 attacttgaa acgtttgacc tgaaacaggt cgacggcaca ccggcaaaag tatttatcat
    33481 gcgtattaaa actaaaacag cgcctgttat ccgcatcatg tattttgcag gcaattatca
    33541 aacaacctat aatcttattg ccaacattcc tgaagtagtg tcgaatttga tcaggaatgt
    33601 aattcttgaa agctttagaa ctcttgaata ttaaatcgtg ttatatgaaa aaaattatac
    33661 actttattct tattcccctt attttttatt cttttggcct gaatgcccag actaccacgg
    33721 ttacgtatcc ggcaaaattt cataccgaga atcagaatta ctgggggccc aatgctacag
    33781 aagtcaatgc gcacgaaatt tttcttgatt atcatttacc ggtaacaaca ttgggatgga
    33841 actatgtata taaccttgcc ggagggcaat acggtgttgc ccttggggcc ttcacctggg
    33901 ttaatatagg tgccggatta gacatccatg aagacagcgc cagagtaaag gtggattatt
    33961 tagctgatgt taaattgcaa atgcctgatg ccggaacttt tgataaaggc gatgtggtaa
    34021 ctattcatac aagccttgat cctgttgagg atagctgtaa gattgagtac gacccatata
    34081 aattggatat ggggttggct ctggattttg gacttggtat ctggatagga gcacaggctt
    34141 gttggggatc gtgcgagggt attaccttgt accaatatga tctgcccgct ttttctgtta
    34201 gtttgatgaa tctttcaagc ggttctttaa caatcggaaa tcagaccatt aatgctgaaa
    34261 tatttccgga tatgcctccg gtattgacaa attatcttta tacacatgat tctctttcgc
    34321 ttggcatccc aacgatgcct tttaaaatag tcctgaaata tccgtccctt cactcggaca
    34381 ctaacgatac ttttttgtcg ggagatactt tgatttgtca atctgcacgc ccctttaagt
    34441 atatgcagct ggatatagat gttatcaagc tgataggtaa aattttggcc aaatcccccg
    34501 agccggttac tcagacagca ggtcagatac ttagtaacct gagcaataat attgatttaa
    34561 gcattatagg cattcctgtg catgtaggtt attcaataat aaggtcggga ataagactga
    34621 aaatgtatca taaccgaaaa cttaaattta tcccgaccat tagttcccag atgaaatttc
    34681 caacggttgt tgattataag gtagtaagac cggataatac gatagcttca cagggaagcg
    34741 attcattaat taattataag gtgggcgaag atatccggtt tacttacccg tgcaattatg
    34801 atttcatgaa tgtttcttca agttattcga tgacaaacca atttacgaat catacctggg
    34861 ataagtacct gatgaatttt ttcgttgaaa tgttgtggat taatcttgaa cttccttctg
    34921 ttactattat tcccgaaata tgtataccgg tatattatcc atgcggacca tggtattgct
    34981 atgtttgcga ctggtgctat ggtggagatt tttgtacccc gccggtagtt tttcccggag
    35041 ttgattttga ggcagggcct ttggtgaatt gggaacttcc tataaattgg aaaagttttg
    35101 attggacaaa taatacctgg gctttgaatg ggttcaatac ctacttggca cctgttcctt
    35161 ttacattaac tcccaggcca tttactgtta cggcaacttc gatacccatc aactgttacg
    35221 gacagaatac cgggagcgct accgctacag ttacaaacgg gaaaccacca tacacctatg
    35281 aatggtcaaa cggagtaacg catacttctg taaataccac cgacgtggtg aacaacctcg
    35341 gcccgggaac ccaatatgtc attattactg attataacgg ctgtcagaca tttaccgatg
    35401 tgatcgtgaa tcagccatcc gccgctcttt ccgtgaatgc aaacattatc caagcagact
    35461 gtaacggcgc cagtaccgga agtattgacc tgattcctgc aggaggcact gccccctata
    35521 cctatcaatg gaataccggg cagaccgcag aggatatcgc tacattacta tcaggtaatt
    35581 atattgtcag tgtaacggac cataacggtt gtgtttccgt gcagaattat acggtgcaac
    35641 aaccgcctcc tcttgtgctt acaccggcat tttccaatgt caactgcaat ggagatacca
    35701 caggtacagc atccgtgctt gtgtcgggag gtgtaacacc atatacgtac ttatggcctt
    35761 caggtgaaac ggttcccgat gttacaggac tgtccgctgg ttcttacact gtgagcgtaa
    35821 cggatttcaa cgggtgcatg aaaacccagg acattcagat cgctcagcct gccgcaccgg
    35881 tttcgcttag tgtgtcagca cagaatgcac tttgttacgg agatgcttcc ggatatataa
    35941 ctatcatccc ggcaggagga actgcaccat acactgccac atggtacaat ccctcaggac
    36001 agattttgaa ccagaatacc gtcagccttg attctcttgt ggccggaact tataatgccg
    36061 tgattaccga cgctgcagga tgcacaacag attcaagtat tttaatttct cagccttctg
    36121 cggtaacatg gggctttagc acggtaaata acctttgttt cggggaatct aacgggtctg
    36181 cagttatcac agccaccggc gggagcgcac cctatgctta tagctggtcg aatggctcaa
    36241 ctgattcaac agcaaccaac ctgcacgcag gtacctatta tgttaccatc accgacaata
    36301 atgggtgtgt tcaggcaaca tcagttacca taatggagcc agcttcagca gtttcagcca
    36361 ctgtcgcgcc gacacatatt ttatgcttcg gtggctccgg cggtatggca aaccttaacc
    36421 caatcggtgg aacaccacct tatacctatt tatggtcgaa cggatttacc ggcgaagacc
    36481 tgactggtgt tcctgccggt gcatatactg ttaccgttac agacaataac ggatgtatta
    36541 catatagcgg cacggtcatc aacgaaccgg ctgattcgct ttcaacgcag ttgagtattg
    36601 ttaaccccag ttgtcatgga tttaacaacg gaagcatcag tattcaagcc tccggtggca
    36661 catcacctta ttatttgagg tgggatgaca cggattttct gttaagcgcc acggggcatt
    36721 tattaacagg cctgccggca ggaacacatc agatcatcgt aacagacgcc aacggctgcc
    36781 aggctattca actcgttacc ctaacagcac cggaggagat tctccttgat acggtgacaa
    36841 caatagttaa ctgttttggc ggatcagacg gtgctatcga cctgactgta agcggtggaa
    36901 cattgccata tacgtatctc tggtcgaacg gagaagtaag tgaagatctg tcaggtttgc
    36961 ctgcaggtga ttattcggta acggtgactg atgcacaggg ttgtatcaca aattttacca
    37021 gcagggtggg gagccggcct gagatcgaga tttcttcaac aacagtgccc atcagttgta
    37081 ttgataataa tgacggaagc atagtggtga tttcatcggg aggaacagga aatataacat
    37141 atatctggtc aaatggtgct acggaaggca acatccatga tcttttggcc ggaaattatg
    37201 ctgttacttt gacagatgca ctgggttgta cgaagaattt cgaatttacc gtaccgtcca
    37261 gtttgttcga atgtattttc ataccctcat cctttactcc caatgacgac ggcacaaatg
    37321 atacatggat catcaggaat attgaccttt atccggaaaa catcgtgaaa atattcaacc
    37381 gctggggtaa tcttctctat gaaagatctc catataatga accctgggac ggaacatata
    37441 aggggtcacc cttaccttcg gaaacctatt attatatcgt tgatcttaac aacggaacaa
    37501 aagcatttac cggaacagtt acaataataa gataattaca catatgaaac gaataacttt
    37561 tttcctttta ctcattgcag catcatcttt tgtttttgcc gacaagcatc cggaaaatac
    37621 aaatgatggg aatagccctc ctccgtgcca taccgctttg ctttccaaaa ccaacgttag
    37681 ttgttacggg ggagcgaatg gcacagctac cgtgaccatc agcaaggcag gaacctacag
    37741 ggtgatatgg tcgaatggtg tcacgattaa ttcgactaca tctttaacgc atcagattac
    37801 ctcgctgccc gcaggatact atgatgtgca ggtcatcgat ctggtgaatg gttgtgctgc
    37861 atttgatatc atcgaggtta cacaaccggc attgcttaca acaagtaata catcggtaga
    37921 cgtaaaatgt tatgggcagt caacaggtag tataaacctt agtgtaagcg gtggtaccac
    37981 gggatatact tatagttggt caaacggtta tacttctcag gatatatcca atattgcagc
    38041 cggaaattat cttgttaccg taacggatag caagggatgc aaagcatatg acagtgctgt
    38101 tattgcccag ccggcccagg ctataggtca atcattaaat attgtaaatc caaaatgtac
    38161 cggttcgctg gatggatcaa ttgatatgac tgtatggggt ggcactgctc cttataccta
    38221 tttctggaat agcacatatc tttcgcagga tctgaataat attggtgcag gcaactattc
    38281 tgtcgtgatc actgatgcca atggttgcat actgaatggt tcaggtaccc tcaccaatcc
    38341 acagctgctt gcggcttctt taacctttac agataacctt tgctacggcg atatgcatgg
    38401 cagtattgat ttgacggttt ccggaggaac acccccgtat tcctattcgt gggccaattc
    38461 tgactttatg ttgtcgtggg ccacacagga tgtcagtaat cttcctaatg ataaatacta
    38521 tgtcaccgtt accgatgcaa acggatgtta tcgggttgat tccgcactca tcagttctcc
    38581 ctctgaaata gtaagtacta ttgcccatac agatgttact gcctttgggg gcatgaatgg
    38641 ctctatcaca cttagcgtga caggtggcgt acttccgtat tctttttcat ggtcgaacgg
    38701 cagtaccagt cagaacctaa gcggtatacc ctcatcctgg tattatgtga ctattaccga
    38761 tgcacacggc tgtattcatc aggattccgt ttttatctca gaacccttaa gtgcattggt
    38821 tgttgatatc atagctcagg atgccacctg tttcggatac agcaatggaa gcattacatc
    38881 cacaaccgct ggaggtactc ctccatattc cttcctgtgg tcgaccggcg ccagttctga
    38941 agacatttca aatgtctctg caggtattta taccattact gtttatgatt tctatggaaa
    39001 ttctgcttca gatactgttg aggtattcca gcctgaagcg ttcacattcc cttatacagt
    39061 tactgatgtt tcatgtaatg gcttgtcaaa cggagctatt gatgtatcgg ttaccggtgg
    39121 tacatcaccg tatacgtatg agtggatgaa ttctgaatat gttcttgctg cacttaccga
    39181 agatattact ggcatgcccg cggatcagta ttatctggaa gttaccgatt ccttaggctg
    39241 caccggaagc atcagtatca ccataaacca gccaaatgca ctcaccatac agatagacca
    39301 tactgatgcc atttgtttca gcagtgctac gggtactgca agttgtatca tctcaggagg
    39361 cacaccgcct tataattatt attggtctgg gggccagcaa acgtccgacc ttacaaacct
    39421 tgttgcaggc atttattatg ttactgttac cgatgccaac ggctgtatta ttaccgacag
    39481 cgtggtgatc agccaggttg attctctgac ggttcaatac gtgatgtctc cggtcagttg
    39541 taccgatcag cacgatggca aaatcagcat cacggtttca ggaggcaacg gcgacttcac
    39601 ctatcaatgg agcaacggcg catcacttcc tgatatcgaa gagctttatg ccggtgaata
    39661 tgaggttacc gtaacagact tcatgggctg taccgcaata aaatcggtaa cagtgacaaa
    39721 aattgagatc gactgtataa atattcctac ctgtttcaca ccgaatggtg acgggtttaa
    39781 tgatacctgg cacataaaag acgcggaatt atatcccgga tttttccttg agatatacaa
    39841 ccggtggggg cagttacttt atagtcttaa tggaacttat gaacaatggg atggaaacta
    39901 taaaggaaaa cctctaccct cggaaacgta ttattatttc ataaggatta gccccgatag
    39961 tcctgtttta cagggaaacg taacaataat cagataaaaa tcacaggcca tgaaaaaaat
    40021 agtaacccta gtcgttttgc tctttgcatt taatgcctta aatgcacagc aattgtcact
    40081 caccagccag tacatgatga atcattatgt gcttaatccc gcaattgcag gaactgcaga
    40141 ttatatcccg atcaacctta acatccgcaa ccaatgggta aatatcaagg gggctcccgt
    40201 tacgcaatcg ttttctgctc atgcttacct gggcaaaaat attggaatcg gcggatgctt
    40261 ttttaacgat gtaacaggcc cgacaagacg aacgggtatg agtgtctcgg tggcctatca
    40321 tctgaggctg agtaaagatt attcacgcaa gctgagtttc ggcattggtg gggttttctt
    40381 ccagcactac gtcgaccggg ataaactttc cactgatgta cctgaagata tgtccattct
    40441 ggatggattc aataatcagt tttgtccgga tgtcaatttt gggatttatt tttcccagac
    40501 tgagaaattt tatgcgggat tttctgtttt gaacctgttt gaaataaaaa aggatctttt
    40561 cactctgtct aacaggatca agaaccctat caagcggacc tattacttca acggaggcta
    40621 cttttttgac ctgggaaaag attttggagt tgaaccttcg ttactgatgc agtttcagga
    40681 aaccacgcct cttcaggttg atcttaatgc aagattttca tataagaaaa tgatatatct
    40741 tggcgcttct tatcgtataa aagatgctgt agtcatcatg tttgctttca atatgaaaaa
    40801 atttgctatc ggttattcct atgacctgac actgagtgac ataaaaaaac attcctacgg
    40861 ttctcatgaa atcaatctga cctaccgtat ctttaaaaaa gacgcattta aaaaggatta
    40921 taattatcca ctgttttaaa caaggctata aattatgagg ggtaattcca tacatgtttt
    40981 tctgatatta tttttgtgtt cgttcctgag taaggcacaa atgaataaag ccaatgaatt
    41041 gattgacgtg ggcaattata cagaagccgc atttatcctg gaaagtgtcg atgaaaaagg
    41101 tactgctgat cagtcggccc tggtcaatct tgcttattgt tatatcagga tgcacgatta
    41161 caacaaagca gcgcagatat atccccggat aattgctgca aaaaaaatag accagataca
    41221 ctatttttat tttggcgagg tgctgcgatc tcttggtaaa tatagcgaag caaaagaaca
    41281 gtataataaa tatcttaaaa aatttcctgg ggatttcaaa tccgtaacat gcattcgttc
    41341 atgcgattcc cttgttttat ggaaaagctt atcttctgac aacaaagtcg aaaatgctaa
    41401 tgacattaat acctcttttg atgaattgtg tcctgtttcc accaaagatg gcatatttta
    41461 tatttccaac aataaagatt tactggtaaa tgcgggtgct aatactgatc tcaacgaccc
    41521 gcaagtgtct tttatttttc agaaaaaaga gggacagacc acggtattca ggccatacag
    41581 tgacagtata agttatctcg cctattcagt cagaaacggc aaatatgcga tggttgtcaa
    41641 accggtacgg aaaactcctg atggtttttt tgccggaact cccgttttaa aactcagtaa
    41701 tgacaataag gcctggaata atttccagcc cgaagaaact cccgaaggat atacgataac
    41761 acatccttgt ttttctgaag atggaagccg tatatatttc gccagcgata tacccggcgg
    41821 atttggaggt tcagatattt attattcgga ctatgcaggc ggtaagtgga acaagcctgt
    41881 aaacctcggg aataatatta acactcctgg gaatgaaatg tttccgtaca taacggaaaa
    41941 aggagacagg ctttttttct cctccgacgg gcatccaggt tatggcaatc tcgacatctt
    42001 tgaatctgtt tatacctatg gtaactggag caaccccagg aacctgaaat ctccggtaaa
    42061 ttcagtgggt aatgattttg gctttatttc cggcaaagat gcataccatg ggtattttgt
    42121 ttcgaatcgt tatgaaactt ctgtcggggg taatgatatt tatacgtatt cctttgtgat
    42181 gcctgagcca aaggatacta cgccagtgtt accaccatcg cgacctttca tcagcgacac
    42241 agtttttgtg tttttcaaaa cggcctcttc gttcattgat cctgttttta ctagtttgct
    42301 ggattcaatt gccggaatca tggctgatga tccctctctt aaacttaatg tggtaagttg
    42361 ggccgatgta catggctctg atagtatcaa taagaatatt tgtaatgaaa gagccggggc
    42421 ggtagcaaac tggttcacct ctaaaggtgt cagtttgggg aaaataatta aaaaaccatc
    42481 aggagtgtcc agacgacctg aaataaaaca aatcgactac cacgttcaaa ttggcatttt
    42541 agccaaagcc ggggcacagg actattatag tcagataatt aaaaatgaag cgctggttga
    42601 ttgtcttaaa tccggaaata aatattatta ttatgcaggt aatggtacgc tggatgaaat
    42661 gaaaactctt caggaaaact tgaaaataaa gcaccggttt tcaggcttta tctgtgcatc
    42721 ttattgcaat tatttcctgt tggatgaaaa atatgcgccc aaccggagag ttgatattta
    42781 cttctccaaa aattagtatt agcaccaggt ttttctttaa aggtgtaaat gttcgtatgc
    42841 ctgactcagt cgttacgtgc tggtctgatc aatttgttgc aagaggatta tatccataac
    42901 ataaggaatt acacgtggtc ttattgttta ttattttttt taccgtatat cggaaataat
    42961 ttgtatttta gcattgaata ttgctgtgaa ataattttat gggcacctct aaaaacttcc
    43021 agtatctttt tttgtctttg ctcttcaaat ccttgcgtca ggaaggctca gcacaggtat
    43081 gtatgcgcag tacactgccg ttttctgaac ttcgactgtc ccgaactttt ttatttttag
    43141 aggcacccga tctcaaacac tacacttata aagacttttt gcaagttttt taagtcgtga
    43201 aattttttgt tgtaacccaa aaattgtcta acaatttaaa tattaaatac tatgagaaaa
    43261 agtttattaa agaatgcgtt tttattcgta tcggtatgtt tgatgatgcc tttactttca
    43321 ttttcacagt ttgttgaaaa caacggacaa atagcacatc gggagatttc taagttaagg
    43381 cctgatatta aatttcaggt aaaaggaaat gaagtgatat caaattataa ggccgatggt
    43441 atcgaaaatt actattgcaa agtccgtgaa gtgccaaggg aaaactggac agatgatatg
    43501 aaagagaacg aaagaatgga cagaaaagta agaagttttc aggacatctt cttctatcgt
    43561 tttgacgtgg ctttcattgg ttcaaatccg gatgtcagta ttgaaccttc tgataaatca
    43621 aagacatata gaaattatta ctatgaattt tgtcctgatg gaattctgaa tgttccggaa
    43681 tataaaacaa tcgaatatca ggagttgtat ccgggcattg acttgcgtta tgtaggaact
    43741 gataagggga ttaaatatga attcattctg gcgaaaggcg caaaaatttc cgatattaaa
    43801 atgaagtata ttggtgtcga cggattgaat gttgaggaaa acggcgacct gacatattat
    43861 attgacggaa agaaaatgac tgaaaaaagt ccgtttacat tttattccgg ctcagggaaa
    43921 gagatcagtt cggcatacac ccttattcag gatgacatta tccaatttaa agttgatggt
    43981 atcgatgaag taactgaaga tatcaccatt gatccggtac ttacctggag cacttatttt
    44041 cagggtgttt atggcggtta tcaaggtgat cccggagtgg acaaccaggg aaatgtttac
    44101 atctgtacag aggaaggggc aggaacctta ccggtgttga atgcaggagc cggccaatgg
    44161 tttcagatcg catctaccgg tccgtcaccg aactgggacc tggcgatttt caaattcaat
    44221 tctgatttgt cgctggcctg gtgtactttt tacggagggc aggcatgtga acaaggatgg
    44281 ggaaagagga atgtagccat agataatacc aataacagga tttatttttc cggtgaaaca
    44341 gataccccgt acagtaattc cgtacagact tttccaacct ataatcccgg aggcggagca
    44401 ttctatcagg acgtttctta tgataatggt tccagttatc cgttttttct tgaattcact
    44461 cccgcaggtg tcagaagttg ggcaacgctg tttacttatg aaacagctcc tttctcaggt
    44521 ggaatgggta ccgatgtcga atctatttat ttcaggaatg gttatctgta tttttcagga
    44581 gagaccaata gatatggcca ctcaggtttt ccattaagaa cactggcggg agcatataat
    44641 aattcatact ggccgggtga tggccagcat atttactgcg gcaggttcaa ctcttcgcgc
    44701 accttagaat ggtgcaccta tgtgaacaat aatgttgcca ctacttcttt cagcagcaac
    44761 ctcggcgata tggtggtaga tgcgaacaat aatatttatc tggtgaccga ttacagtgag
    44821 tattctgcct ccaagctcag cacaattctt cctctggtga atcctggcgc aggtgcttat
    44881 tttcaagcca ctggcggact tggttatggc tcacgacgtg gtacgcacat tacaaaacta
    44941 aatccctcaa gctctactgt tttatggggc acattttata acggtgggta tggagacctg
    45001 ccgggtgttt gcgaagtgga tgcatcaaac aatttttatg tcgggtggag gtcaacagga
    45061 agcactgatt ttccttgtta taatcctggc agcggtgctt attatcagtc cacaaaaaga
    45121 agtgtttgcc ttactggtcc gaatgattat gctaacggag gtaaagatgg tgctgtcgcc
    45181 aaatttactt ctgccggagt tcggctttgg gcgacatata tcaatggaac aacaccaacg
    45241 agtggtgaaa cgggtgtaaa tgatattgat acctatccta ccggcgaaat gatgatgtca
    45301 agcaaaacag tatgctccga tttcccggtt cagtatcttt caggatcata taataagtca
    45361 acgatcacaa cttccgggac ttatgaaaat gtttttacaa aattcagctc aggcggagtg
    45421 attctatggt cttcttattt tcagccatta atgggatatg gtgccattca gaataacaac
    45481 tgtgtgagta aatattttgt tgataacggg ggcgggagta ccattccttt ggttaatcct
    45541 ggatcaccgg cgtattacag ctctacacct cagaattatc agtcatttca gttgtttgaa
    45601 gatgccacat ccaatcaatc aactccagct tcgtctattt ctgcctctcc aacaactaca
    45661 atctgcaccg gcgggagtgt tactctgacg gttcagggtg gttcactcgg tagtggagca
    45721 agctggcaat ggttttcagg tagttgtggc ggtacttttg ttggaagtgg agcatctgtt
    45781 aatgtcagtc cgacttcaaa tacaaattat tatgtcaggg ctactggagt ttgcaataca
    45841 acttcttgtg taagcctttt ggttacggta acaccaaata acacgatctc attgtctgct
    45901 ggtggcaccc agacaaaatg tatcaacacg gcgatcacaa caaccacgta tgccaccacc
    45961 ggcgctacgg gagccactat cacagggttg ccaaccggcg tgacgggtag ctgggcttca
    46021 aatacggtaa ccatcagtgg cacaccaacg gccgccggtg tatatacgta cacggttgct
    46081 ttgacaggcg gttgtggcac tactaccact acaggaataa taacggttac tccaaataat
    46141 accataacac tgtcttcagg caataataca cagacccggt gtatcaattc ggctattgcc
    46201 aacatctctt ataccagtac cggggctacg ggcgctactt tcagcggttt gccatcgggt
    46261 gtgtcaggta actattcaag cggcaatatc accatcagtg gaacaccatc agccagtggt
    46321 acatttaatt atacagtcaa cttaacaggc ggttgcggaa cagtatcagc cacaggcacc
    46381 ataacagtaa ctccaaataa tacaataaca ttgtcatcag gcaacaatac acagacccga
    46441 tgtatcaatt cggctattgc caacatctcc tataccagta ccggggctac gggcgctact
    46501 ttcagcggtt tgccatcggg tgtgtcaggt aactattcaa gcggcaatat caccatcagt
    46561 ggaacaccat cagccagtgg cacatttaac tacacagtta ctttaacagg cggttgcgga
    46621 acagcatcag ccaatggaac aattactgtt actcccaata ataccataac actgtcttca
    46681 ggcaataata cacagacccg gtgtatcaat acggctattg ccaacatatc ctataccact
    46741 accggggcta caggtgcaac tttcagcggt ttgccgtcgg gtgtgtcagg gagttattca
    46801 agcggtaata tcacgatcag cgggacacca tcagctagtg gtacatttaa ttatacagtc
    46861 aacttaacag gcggttgcgg aacagtatca gccaatggaa caatcacggt gacaccaaat
    46921 aataccgtat cagttgcatc ctcgacacca acattatgca ttaatactcc tttgactgct
    46981 ataacacata ctacaagcgg cgctaccggt attggatcag ccacagggct gccagccgga
    47041 gtgacagcat catgggcatc gaataccatc actatcagcg gcactccgat gatatccggg
    47101 accttcagct acagtattcc gctgacagga ggttgtggta cggcaaatgc tacagggaca
    47161 ataacggtaa taccaaacaa tacgatcacc ttagttgccg gtggcactca gacgaaatgt
    47221 atcaataccg cgatcacaac aacaacctat gccactactg gggcgacagg agctaccgtt
    47281 actggattac cttcaggcgt cagtggtagc tgggcttcta atgtggtaag catcagtggt
    47341 tcaccaacag tgtccggcgc atttacctat acggttacgt taacaggtgg ttgcggaaca
    47401 gtaacagcca atggaaccat aacagttacg cccactaaca cggtaactgc tgcttcttcg
    47461 acgccaacac tatgtatcaa tactgctctg acagccatta cgcatacgac aagcggcgca
    47521 accggcattg gaacatcttc ggggttgccg gccggggtga cagcatcatg ggcctcaaac
    47581 accatcacta tcagcggcac tccgacggca tccgggacct tcaactacag cattccgctt
    47641 acaggtggtt gcggaacaac aaatgccaca gggacaatca cagtaatacc aaacaacacg
    47701 attaccttag ttgccggtgg cactcagacg aaatgtatca ataccgcgat tacaacaaca
    47761 acctatgcca ctactggggc gacaggagct actatcacgg ggttaccttc aggcgtcagc
    47821 ggtagctggg cttctaatgt ggtaagcatc agcggttcac caacagtgtc cggcgcattt
    47881 acctatacgg ttacgttaac aggtggttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48001 nnnnnnnnct ccgacggcat ccgggacctt caactacagc attccgctta caggtggttg
    48061 cggtacaaca aatgccacag ggacaatcac agtaacacca aacaatacga tcaccttagt
    48121 tgccggaggc actcagacga aatgtatcaa tactgcgatt acaacaacaa cctattccac
    48181 tactggggcg acaggagcta ccgtcacagg tttgccctct ggtgtaacag gaagctggtc
    48241 ttcaaatacg gtgacgataa ccggtactcc gacagcatcc gggacatata cctacacggt
    48301 aactttgacc ggtggttgtg gaacggttac agcgaacgga actattatgg taagtcccat
    48361 aaatactatt acgctgatag cgggtgcgcc tcagacagga tgtatcaata cagcaattaa
    48421 taacattacc tatgctacca cgggagccac aggagctaca gtcactgggt tgcccgccgg
    48481 agtaacaggc acctggatgt caaatgttgt tgtaatcact ggcacgccaa cagtattcgg
    48541 atcttttacc tacacgatta ctctgaccgg gggctgcggc tcggtggcta ccacacaaac
    48601 aattacaata actccaaaca gtacggtaac actgagttct gcatctggca ctgatgcaca
    48661 aaacatatgc aacaatactg cgatcactaa cattacttat gccaccacca ctgccacagg
    48721 agccactgtt accggtttgc cggccggcgt aacgggaagc tggacctcga atgtagtaac
    48781 gataagtggc acgccgacag tgccgggtac ctatacctat acggttggtt taaccggtgg
    48841 ttgcggaaca gcgacggcca cgggaacgtt gacggtaata cccgttaata cgataacact
    48901 gacagctggt ggaacgcaaa ccaaatgtat caacacggcg atcaatacga ccacatatac
    48961 caccacaggt gcaacgggag ctaccgtcac cgggttacct gccggggtta cgggtagctg
    49021 gacgtcaaat gtggtaacaa taacaggtat accaacggta gccggagtat ttacctacac
    49081 catttccctg ccgggagggt gcggagtgtc aacagccaca ggcataataa ctgtgacgcc
    49141 tgacaactcg ttgacactaa gttctggagc aggtactaat gcgcaaaacg tatgtattaa
    49201 cgcaccgatc accaatatta cttatgccag cgcggttgcc acgggagcca tcgtcagcgg
    49261 tttgccatta ggtgtaacgg gcggttgggc ttcgaatgtg gtaacgatca gcggcacacc
    49321 gacggttaca ggtgcatata cctatactgt aacaaccacg ggtggatgtg caacggcaac
    49381 agccacaggc actttagtgg tgaatgtaaa caagactgcc gatgtcagcg tgcaggcatt
    49441 tgacaatcca gcttgttcgc atacgatggt cacttttacg gcaacacctg taaacggagg
    49501 cctctcacca gcctatcagt ggtttgtgaa cggtaatcct gttggtggca acagcagcac
    49561 ctatagcaat atttctctaa gtaatgctga tgctgtagta tgtcggctga cttctaacga
    49621 attttgtgcg ataaacaatc ctgcttatga cacggtcaat atgatagtta atcctatacc
    49681 gatagcagaa gcaggtacca acgctacata tacgggcact ccaatactga taggtgatcc
    49741 gttaaacggt ccgggagtta tcagttggtc gccagcgaca gggttaaata atacatgggt
    49801 cgcccagcca ttagcatcgc cgtcagttac tacaacctac acactgacta ttaacaactt
    49861 tggttgtata cgtacagata ccgttactgt tcattttggg ggattaggac atgttatcag
    49921 cggaaaaaca cgttatctta aaaagggaac ctcaggaacc ccgtcgccta atccgcccac
    49981 atacaatgct gttatatata atatagataa tgta
//
LOCUS       DAOPYB010000004        49446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_564_length_49446_cov_8.209492, whole genome shotgun sequence.
ACCESSION   DAOPYB010000004 DAOPYB010000000
VERSION     DAOPYB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 49446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49446
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_564_length_49446_cov_8.209492"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..274)
                     /locus_tag="PKH58_00545"
     CDS             complement(140..274)
                     /locus_tag="PKH58_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87542.1"
                     /translation="MKSRTKIRLIQYEIAGFIVFVDVLVLYIATHLNETKQFVENLFS
                     "
     gene            complement(451..2100)
                     /locus_tag="PKH58_00550"
     CDS             complement(451..2100)
                     /locus_tag="PKH58_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNX87543.1"
                     /translation="MKKYILSLIILVLVTGFTSSDKKTKIFIAGDSTAQTYKTEKDGL
                     IKGWGQMLPEFLDDNVEVVNHAMGGRSTKTFLNEGRWDKLMAQVRKGDYVFIQFGHND
                     ASTRPERHASRADYRDNLLKMIQDVRDKKAHPVLLTSVVMRTFVNGNLTDDRLKEYPV
                     IMRQIAREFNVPLIDINQKTRDFITVLGDKKSVAYYRHSEPGEDPAKPDGVKDDTHMM
                     KKGAEQVAYFVAEGIKELKLKGLCNHIKQGVEDQGIGSILPVRNKYVYLFSYFKGNGD
                     GLHLAYSYDGLKWEALGRDTVFLKPVVGKDKLMRDPSIVQDDNGVFHMVWTSGWWDNG
                     IGYASSKDLKYWSEQKYIPVMEEFAGVKNTWAPELFYDRKDKLFYIFWASTVPGAFPE
                     IPTSTSEKGLNHKLYYVTTKDFNTFSETRLFYNPGFSVIDGTMMERNNRYYMFVKNEN
                     SNPAEKNIRVVSNNKPLRFPNKVSAPITGNYWAEGPASLQVGDYVYVYFDKYRDKKYG
                     AVRSSDLINWEDVSDSVTFPKGIRHGTAFTVTESVFNQLTK"
     gene            complement(2215..4992)
                     /locus_tag="PKH58_00555"
     CDS             complement(2215..4992)
                     /locus_tag="PKH58_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNX87544.1"
                     /translation="MKKKTCCLLIIMFFAAIHAMASDTGWPAVTSESKPWTRWWWLGS
                     AVDKDNLSYNISKLGKAGLGGVEITPIYGVKGNDANNISYLTPRWMEMLAHTENEARN
                     SGMKVDMNTGTGWPFGGPEVTLEDAATKAVFQKYEVSGGKNISLNITVEDPKQRKVAY
                     LSRLMAYAATGKKLDLTKLVSKEGNLNCKLPKGEWQLIALFVGKTLQEVKRSAPGGQG
                     YVMDHFSAKAVKNYLTKFDSAFARDNTPCPNTFFNDSYEVYNADWTPTFLEEFQQRRG
                     YKLEDFFPELLGKGKTDISCRVVSDYRETLGEMLLTDFTAQWTSWAHSHGATTRNQAH
                     GSPANIIDLYAAVDIPECEIFGITDFDIPGLRKDEMRKINDGDPVTLKMASSAAHITG
                     KKFTSSETFTWLTEHFRTSLSQCKPEIDQVFTSGVNHVFFHGTPYSPREAAWPGWLFY
                     ASVNMSPTNSIWRDAPAFFSYITRVQSFLQYGQPDNDLLVYFPVYDIWYEQQENYYFA
                     FGIHGLRARLPKFYNTVEKIRESGRDVDYITDRYIMSATVENGLIKTIGGTTYKALVL
                     PATRFMPPETLEKLSHLAREGAKIIFTEHYPESVPGLKDYRKRENRMKKMINTLPEAN
                     FAKSEMSKWGNGNIITGNDFNELLTMSGIYSESFSQSFKGQYIRRKNETGYHYFFTQL
                     TNNPVKNWVPLAVHAQSALFFDPMTGTSGKAKIRTANNATEVYMELEPGQSIILKTFT
                     DKNMEAENWHYFIPDGQKMTLPDNWEITFKESEPAIPGVFKTGKLISWTELANENATK
                     NRGTARYSTRFIWNDLSADEYRLSLGDVRESARVYLNGNEVATLFAVPFRTNIGKYLK
                     QGENLLEVEVTNLPANSIRDYDRRGVEWRIFNEINFVDINYKKTLYDKWETAPSGLLG
                     PVTIQGLRYER"
     gene            complement(5144..8032)
                     /locus_tag="PKH58_00560"
     CDS             complement(5144..8032)
                     /locus_tag="PKH58_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX87545.1"
                     /translation="MKRSMTTFKLRGIFILAAFFSLCASGQNLTIDLSGKWRFDIDRN
                     NVGVSEKWFSKTLRDNIYLPGSMTENRKGDDVTLNTQWTASIYDSTYFYNPRLKKYRQ
                     PDNLKLPFWLTPVKYYAGAAWYQRDIFIPADWNNSRIVLFLERPHTETRLWINETEVG
                     MQNSLSVPHQFEISKYLKKGKNRITLCIDNRTKDIDVGKDSHSITDQTQGNWNGVVGR
                     IELQAMPQTYIEDIQVFPDLKNKKAVVRLNIEGKLSGIITLSAESFNSDKIHKITPVS
                     KELSLKNSVGKMEIELPMGDDFLTWNEFHPNLYKLTVTLKTKQGTDTRDTRFGMREFT
                     IQGKYFYVNGQKTVLRGTVENCVFPLTGYAPMDEKSWERVFRICRSYGLNHMRFHSYN
                     PPEAAMKAADLVGFYLQPEGPSWPNHGSSLGDGRPVDKYLWEEAERIVKQYGNYPSFC
                     MFAIGNEPRGRWVPWVSKFVNYWKEKDPRRVYTGASVGGSWAWQPASQYHVKAGARGL
                     DWDRRPESNSDYRGRIDTVRQPYVSHETGQWCVFPDFREISKYTGIMKARNFELFRED
                     LTDRGMGYQAADFLMASGKLQALCYKHEIEKTLRTPGYAGFQLLSLNDYSGQGTALVG
                     VLNAFWENKGYINAEEFRKFCAPTVLHSRMDKFIFKSNEDLKAGIEIAHFGEETLTGK
                     QTEWKVRNELGKIVYEGSFAPRDIQIGNCQQLGEITLPLHSIPEAEKLNLEVQLKGTD
                     ISNNWDFWVYPETLPLVDTTSVYICDKIDDKAKQVLIEGGSVLLQMAGKITQGKDVVQ
                     QFVPAFWNTSWFKMRPPHTTGILINDFHPVFKDFPTDFYSNLQWWELVNRAQVMELAD
                     FPVSFQPIVQPIDTWFINRRLAMLFEAKVGKGKIVVCSADIQKDLDIRYVARQLRYSI
                     LKYMNSGLFFPENDVTLEMIENLAIKRGEWLDMSMTKDAPDELKKNIK"
     gene            complement(8054..10609)
                     /locus_tag="PKH58_00565"
     CDS             complement(8054..10609)
                     /locus_tag="PKH58_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HNX87546.1"
                     /translation="MKKVRLCIFLFVLFLCSQTSATGLVWFDGSNPISYSLPRQAAPV
                     VKVAAGMFCDDMLQVTGFAPQQKPENLATIRIVQFEEKLSKQLKNVGIPVDELAGKRD
                     AFFIKVSDKQLIVVGSDARGTAYGILELSRLAGVSPWIWWGDMKPDKKNRLAVPEDFH
                     TLQSPSVEYRGIFINDEDWSLQLWSWKNFDPSDRKGLISAKTYREIFKLLLRLRANTI
                     WPAMHGATVPFYFVPGAKEAADSCGIVIGTSHCEPLMRNNVGEWDEKIRGNFNFPDNH
                     DRVISYWKERLNEVHQYENIYTIGMRGVHDGPMEGVSTLKEKTEALQQVINEQRELLR
                     KHVNPDLSKVPQQFVPYKEVLDIYENGLKVPDDVMLTWCDDNYGYITRLSDEQQQKRS
                     GGGGIYYHLSYWGRPHDYLWLTTTQPGLIYNEMMEAYKHNARRFWVVNVHDLKPAIYD
                     LEFFLDMAWNINSIKNTSIVTHLEKWLCREFGREAGIKLLPAMTEYYRLSNIRRPEFM
                     GWTKVEEDKKKYNRGLTPVIDTEFSLTEFGGELDRYLSDYEQIRNIVQQTESLISENR
                     KYAFFAAIKYPVFGASAMAAKWLEAQRARYIALQDKDTIHWQNNTKLVEACTRSLNAY
                     AEIQTLTDYWNNRMSGGKWKHSMHAAPRDLPVFDAPVLPLKTNGNDTERKYDLPLSYH
                     ATKIDQLEPDSFIARNACHFTRAGKQVKSIQMLGHSMNAVSLPRSESVSFEFDCPWEG
                     EAVLRTAVIPTQPNDKGDIRFSVQIDDEKPKIISFRERGRTETWKNNVLRAQAVKTTG
                     YTLLKGKHTLTITALDNHVLIDQWMIDFKKNRKFYVFPLTPAYGAKQVFLSNN"
     gene            complement(10829..15526)
                     /locus_tag="PKH58_00570"
     CDS             complement(10829..15526)
                     /locus_tag="PKH58_00570"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HNX87547.1"
                     /translation="MKTLGLKKSLVLFLLIITGVLQSGVYAQRVMEKLDRGVVAVKNS
                     SGGYLVSWRYLATDPEDILFNLYARVSGGSGYVKLNASPLTATNYSAASGAIGVNTQL
                     YVTPVINGVEGTQSGIFKIPYTGFPATYRSAYIDINYSPLADGLDLYKYSTKFIWPAD
                     LDGDGEYDFVVDRLSVDGGTHKIQGYLRNGQYLWTVDMGPNVSISQGQDDMVIAYDMD
                     SDGKADVVIKSSDGTKFADGKGVNGSTTLDTDNDGIIDYNSQNVKNPPQYITVIDGMT
                     GKEKNSIEMKYPSNYTRTNKAIFMGTEYANLNGMMAIIYIDGKHPSVGFVYKTRTSSD
                     KYHWYYASAYGYNASGQWVNWYNWERGYVGAAEFHSIRSADVDLDGRDELLNGGYGIK
                     YDGTLAFNANISHGDRFRTGDIDPDRPGLETFAIQQNNPTMLGQLIYDAGTGEAIKKI
                     YMSAVGDVGRGECMDVDSTRLGYEFWSTMANIYDAKGNILYEGGTPFPHEGIWWDGEL
                     DREELSSADGNGFNAHVGKYSVSSHSFGNRLIEFAKMTGWQVNSEYGVRPAFFGDIIG
                     DWREEVLLEKKGSQVVGDSTYTTNTGFVGFSTDYPTTKRIYCLMQNPAYRDQATTKGY
                     YQSPIPDFYLGYNMPVPPVPPVQKAKLTWSSGTAFDKSSTSFLQQDEITRTAFVDGDD
                     VMFDISGDNSAPVQLTTDLAPSKIWAMNPKGKDYVLGGTGKLTGTMELVKSQNGKFTL
                     NGNHTYTGKTIISEGNLCLNGSNNSPIDVRAKGTLSGNAVLNGGIVLNPGLNVEGGRL
                     SPGNGLEAGKLGKIVINGALTLPGKSNIEIDIIPEDPYKNDSLVINGNFTANGINTII
                     VNGTLTPGTYTLVAWTGTLKGTLANFDVTGVAGLPVRLVINSHDLQLVVGATRSASGV
                     TWTGSTDANWDYMTTNFQTITLPHQSTFFVQNDSVLFDDSAVQTNVSLSESMITKDVQ
                     FANNTKSYTFTGTGGIGGSGNFQKSGKGLLDLGNIQSTFTGKTIYNNAQVKVSALNQT
                     SMPGPLGQAATAPANWVMTDTRLIVDAVESNTDRGLTIQGTDTIEIPKSNGVVSITGT
                     ITGTGGLVKSGAGQLNVSSSSPNTYTGETVIKAGTIGLGTLEMNRTGFGKSGKIRMEN
                     GSRIRMFDNNSDYNQKATWYITIPEGSTARIDASSRCDINGSISGAGTLNYYVPYVRA
                     DLVAGGGNNFTGTINVTGRDFRITTNAATFPKAAINLGANVFMGAYSTIGSSSTNAST
                     VVKIGSLAGDATSSVGGGTWQIGTDNRDAIFRGVFGSGATVTKSGTGKWTLTNASTLT
                     STFSVTDGTLVVQNTTGSATGTGNLSMSGSAILAGTGIIGGNANISNSAKVMPGNNET
                     ALGTLTFSKNLSLTVGSTVVMKTISTANDQLKVDGTLYLNGNLELRNLSGNWTAGKSY
                     TLFVAPTISGSFTSITPAIPGKDLIWDLTRLNQGIIAVINDPTPVNEINASSVSVYPA
                     VMDKYCLINLGNITGKVQIDLYGVNGVKKLSYTESGYNSLVRMNVSHLQPGAYFLRLT
                     NSGSTATYKLIKK"
     gene            complement(15645..18365)
                     /locus_tag="PKH58_00575"
     CDS             complement(15645..18365)
                     /locus_tag="PKH58_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87548.1"
                     /translation="MKKLFTLFLGLLLTVSFVQAQEYWDKYLINENFDGAVALPTGWS
                     SLTANTTSTTAVFGRSGGTSLVDGIRFTGSGSGNRGAEIKIPLAPDSSTIYVDLDLLV
                     AKSAISNGNSFQFYLLGSKSVNLWNTGTPYTDVIAGLFWVGGSGKFHLWNKDIKGPVP
                     VNKPDTIIPVFVTGQYPSFRRAGTTTFKTDSLNLSTKTDVGALKNKWYNLNFKLNFAT
                     KKMDLTITQKDSIANTQTFTNLDFVSPTVSDFVQFGMLNNRASNIANHLNADLDATVD
                     NFKVYQKVLSLGKADLTIKYIDQDGNEAKPSKVLTSQEVGLMYRLLESEKASFTSGGN
                     YYAYSAAATGPDSVIVQLGGSTLTAVFKKTPQTSGTYTWKGFTSEYWNELDANFTTNL
                     FNQLAYQNGNGVKFSLTSAAIKDVSFSSKFDLGANSMSIDVPDYTISGTGVLFGTGAI
                     NVNASTTLSFMSKLTGGLNLNKGTLQVKNYYVTNKYNVKSGTTLNVNIGSTVFSTPIT
                     GTGIFTVMPTAQVEYNSTVKGVSEIYYSLPVKGNIAAMNKMPRMNFALDSLAKINVIS
                     ARGDSTTYFGTTLNYKNNRIHLGDSIGMVYSVNPATGGTTNILIGELTGTAKSKLVGP
                     NVRTMTYTVGGLNTNAEFAGELAPINIDAWAGNTTYNIAKTGKGTWTLSGASPYFYGN
                     VKVLDGTLVVNNVLCDGLAKVYTVIPTATGVPTSITLTKIAEVLVADTATLAGNGYIG
                     AIQNTVNGTITGNLTLGGTLSLKQDLGAGGATTIINATASGVDKITIEGDLNYGGKLI
                     VRATGQRPAPGDYKILDAANYIESGEFGFDSIELPSTNWSFDFATGTLTYKGGDDTAV
                     STIDYSKSIDSMEYFDMTGRKVSGYQKGMVFVKVKYTDGTSGVYKTFITK"
     gene            complement(18596..20995)
                     /locus_tag="PKH58_00580"
     CDS             complement(18596..20995)
                     /locus_tag="PKH58_00580"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX87549.1"
                     /translation="MLMISAGSVNTLHSQENLGRGMVAYRSTENNVKYVSVSWRYLSS
                     DNPAVQFNLYMAKVNFDGTLQPYTKLNTEPITNNTFFKYQEKNSVALQYILKPVVNGE
                     EGDSIAGYRLKKYTEGAISNYIQIPMKQVTGDDLWKYSPNDASVADLDGDGEMEIVVH
                     RTGSGQDNANSGITDAPVLQAYKIDGTFLWEINLGVNIREGAHYTQFMVYDLDGDGKA
                     EVVCKTAEGSRDAAGTPVGKAYFPEYKAKYNLSVNYNENAVYRNSSGYILQGPEFLTV
                     FNGLTGTEIVTTEYDPPRYSTWNNGNEIPKLTQTGDELKARWGDNYGNRVDRFLACVA
                     NLGGENHSVVMCRGYYTRTVLVAYDYKDGKLTRRWKFDTWTGSGNTAYAGQGNHNLRV
                     GDVDGDGYDEIVYGSMTVDNNGKGLYNTKLGHGDALHLTDFIPERPGLEVLGVHENKV
                     DGTTLRDARTGEVIFRVPSGDDVGRGMGSDVDPRSRGMEWWSSRTGGIRSSASGEVVS
                     TTSVSMNMAAWWDGDLLREMQDGTGVTKTDPVNNVITTLLDASAECSSNNSTKANPCI
                     QADILGDWREEVVLRTTDNKNIRIYMTTYPTDYRFHTFLEDPVYRMSVVYQNVAYNQP
                     THTGFYFGSDLENIFPENHINIESNSYTVNPVFDALSYKWSTGENTKTIVLNSADYTV
                     DKEYQVSLDMNFRGYLFHDTLFFTFKNGTGIKNLAENRIKLLNNPVKEQLNIRFDEAG
                     SYQLSIYNLTGALLSNESFIVSDKQIRNYNVSDIKSGMYILNIKGNDRNYTVRFIKLP
                     L"
     gene            complement(21176..22951)
                     /locus_tag="PKH58_00585"
     CDS             complement(21176..22951)
                     /locus_tag="PKH58_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX87550.1"
                     /translation="MNTFKYIIISLGLTMVMGLSSCIEADIEEAVEYKDFYSTLDDAD
                     AAVLGIYGKVMGIADRLVVLNELRGDMLDVTTNAGHDLMDINQQKANKDNFWADVTPI
                     YEVIQNCNDALYNFDIMLEKKKLTQDQYNERYSDIGAIRCWLYYQLGVQFGTIPYITN
                     PIVKIADLVDQQENMKNLDELIPELIRFMESLPTLEDYKNSKLITGTIDGVSLIPYFI
                     NKKCLMGDLYLFNNEYDKAATIYRQVLAKNEDAGVTANSTTYRIYDDRSWQSGEVTYF
                     SVLFNRYTPDDGSKLYNSWINMFKLKADDRYVKNEMIWEMSYDSKFAPAYPFIKLFGT
                     EGKGEYYLKPSKYAIDEMWGSERQRNGYPYDCRGVSGGVTKTADGRYAVAKYSLSYDV
                     TGKPYDDAGKWYLYRAGLLHLRFAEAANRSESGSGYPRLAWALVNDGIIGSAFTWRHT
                     DGTTFRGDSTKYSSYGPGRPYPAPYYFDGRFIDQPYFRSAWRYNGGIRGRANLQNVSM
                     SQITTKQDSIAFVENMIVKEAGLELAFEGHRWTDLIRVARRLQKLNGTGGQFLYDKMH
                     RKYELAGLPVPDFSSEANWYLPLYK"
     gene            complement(22999..26142)
                     /locus_tag="PKH58_00590"
     CDS             complement(22999..26142)
                     /locus_tag="PKH58_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX87551.1"
                     /translation="MRIGKYIIPFLLLCVVNYSTVNAQAKDNFTYSVSGIVKSAETQK
                     PLSGIRIAFKDMETTLTNNEGHFSMNLPSGNVNLTVSGPGYVSKVISVKGRKDIAVEL
                     YTEGFRSVFENVTTPQGISSPVELTHTWSAINENNILSTAITSDEALQGKVSGLNLIH
                     HSGALAAGSNIYIRGLNSMNAGIQPLIIVDGMPYENTVYSTSLLGNYFSNPLASIDIK
                     DIENITVLKDGTSQYGVKGANGVILIRTLKAKEMGTKINFHLHTGVNLEPLEIPVLNA
                     TDHKLLISDMLQSTGMSGSQIMNLPYMNMEKPVNQKWGYEGNVDYYRYNNNTNWQKEI
                     YNLSYNQDYYMNVFGGDEVAVYALAVGYMSQDGIVKNTSFERFNTRFNAEVNLSKKLK
                     LFANMSFLFSNRKLIDEGPSTNRNPLYAALVKAPFMASHVNNPEGAVSPVIEDADVFN
                     NSNPYSLVNTSSRDNSLFRFVGNFEAKYDFNKHLKFNAILGVNFNKEREKLYFPNRGV
                     YFDTLSIGPVDNVSQHRVDRLSSIYTEAALSYTNLFNLEHKLDIRGGMRNQTNQAQDD
                     WGKTANTGSDSFKSISYGDPLYRVIGGQISNWNWLNLFATVDYGFRDKYFLNYTMSAD
                     ASSRYGREANPFCLYPSASAAWLISGEEFMKDASLFDLLKLRIGYGLSGNDEIGNYNG
                     VQYYIPQSLLGSFGLVRGNLVDLNLRPEKTAKMNAGVDASFLNERINFSIDVYKNNIS
                     DMILRIPTPRETGFPFYLTNAGKMSNTGIDFSLKSRILNGTLKWDAGFVASVYKNKVT
                     DLKNEEYITEICNGYVLTRVGQPLGVFYGYKTDGVYATQAEAANEGLMIQKGGLTYPF
                     SAGDMRFVNTDNTNKLIDENDRVIIGDPNPDLFGSVFTDLKYKNWSLNVFCTYSLGND
                     IYNYTRAQLESMKNFDNQIQAVLNRWKTEGDMTDIPRAAYGDPMGNARFSDRWIEDGS
                     YLRLKSVTLSYQMPFKSDIIRSCTIFASGDNLATLTGYKGLDPEFALGQSPLYNGIDA
                     TFVPFSRTLSVGVKLDL"
     gene            complement(26177..27952)
                     /locus_tag="PKH58_00595"
     CDS             complement(26177..27952)
                     /locus_tag="PKH58_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNX87552.1"
                     /translation="MKSLVKYMAAAFILFIFTACESKWEEHIAAKDLRGKTLFQAIAE
                     NPQTSVFASILEKTGYDMLLSGDKMLTVFAPVNDALASVDMNDVEGLKNLVKNHLAYS
                     NYTIVEDHFSSDRIEMINSKNYFVNGLQINGISLIKETGKFNLSAGNGILHLMNGVIP
                     GQKNIWEYLQTQTGNLQAKFIKDQDRLIMDMTKSVQTGVDPKSGKPLYDTVWINRNPF
                     LSAYPIDDETKNYTFALLPNEVIQRIETKYAKYFIKENKTLQDSITRAELIKDCILMP
                     VVITADGRYISVDGVMMDISASKILETYAASNGRIYKLSDADVKIYENKVKTIKIEGE
                     NYFSFYADNMNAWMMRYRPTLSGGKDMTLNSPTSYNTQYIYSNPDTTISVAIARTFAP
                     NNSSSNAGRTNNCYIEYRPVINSVAYKVYWSGYDDYSDHINQSVALSIKTGKETVNVN
                     TSVTCMFSQKMLISFPDKPRVSRKASDGSIADNFSETTVLASTRFKAGVQEEKQLYRY
                     LRVEDATQAPFGLLRKNATPTGEDDYFSYFTGTDTFGDKETIICPTFGEATVFVANTA
                     EYKATNCGMVFLDYIKLVPVVDPNE"
     gene            complement(28117..29973)
                     /locus_tag="PKH58_00600"
     CDS             complement(28117..29973)
                     /locus_tag="PKH58_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNX87553.1"
                     /translation="MEHVSNLKVKKSLHMKQLKKNQIQNIIAALPVLFILLAFGLNSC
                     QKDMEGKIYQLSDQKMIDEILESKGDTLSSFLKIIDISGLRGTVHAYGTYTLFAPTNE
                     AVDKYLTENSLTLNSLTEVQAAEIVKYHLIADTIRSFDFVDGRLPATNFSRRYITTKT
                     ISDATGVSIEVNRQGKILQKDLKAANGYVHVVNSVLNQSRKSIKDVINGLPDTYSLWK
                     EVYNASGIQKTIDEAQKTNPDIVFTCFIQDNEGFKSAKIGNMEELMTELRKKSPDIKD
                     DALLLYNYVAYHMTAGFTYVVDLMNKSALNTLVPGEVIVLKKDVNRVLLNEFLIGGVL
                     EPGILVDRTSEYTDMSCSDGVVHDINGNIQIVKRSAFRVYWDFTEQPELMAMKNFRKA
                     GASIYIDNTDCAGITWGKTFATDKLQYYCGGYPSTITKDNNYVYGDYFYFRLSTNTMK
                     WIEFKTPVLVPGTYKVWLSYRGLTTVQQIRTIFKQDGKEDQLLGVISTGYNKTPGSYG
                     LTDYNNEFFQKALLDGNRYQMINSQGFWSNSNNCQAMGIIQVFTTGQHTLRLEPLNSS
                     QFTTWWDQILFIPIDEDQVWPKQDNTGKLIYQETPKCEIYPYSDCSVVPPSE"
     gene            complement(29960..31582)
                     /locus_tag="PKH58_00605"
     CDS             complement(29960..31582)
                     /locus_tag="PKH58_00605"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX87554.1"
                     /translation="MKNKIIIAITILLSTGFSACSGYLDLEPVDGVIVTKFWKNKEEV
                     KDALMGCYASMMTTGAMQDFLFWGELRGDLIKPRVSANNLSAMSQFQNGDISPNMSYC
                     SWGDIYTVINNCNTVLSFAKETQKLDGSFSDQLLSEYEAEAKCIRALMYFYLVRTFRD
                     VPYVTTASLSDAQNYSVPKTDGSQIIDSLISDLKSIDRAELGTNYGIPYTYGSKKEEN
                     KGRFTLWSMKALLADIYLWKDDYENCVSQCNQIINSGQFTLVPVQNTPVEVISISPPD
                     TITAYYPSEGDVDNMFLNMYANGNSVESILELQFGTDHENPFYDLFNPVYGSLVANME
                     MMSSDQLFPPSAFDRGWYDIRGEGVDYKQGYVWKWIGLSRTSYTYRTDGMSFSNWIFY
                     RLADIILMKAEALCQMGKTSGNTAQLVESLELVKTIRSRACAPETSNEIQDENNINPN
                     DLEKFILKERAREFAHEGKRWFDVLRNAKRNNYAGISYLIELAAYAASPDKALSLQSK
                     WGGDYNSHYLPISESELRTNKALTQNPFYGTR"
     gene            complement(31607..34984)
                     /locus_tag="PKH58_00610"
     CDS             complement(31607..34984)
                     /locus_tag="PKH58_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX87555.1"
                     /translation="MKRAFIQMLKIKDLVQCGFHATTIKINNERMKNSIKIYCFVLGL
                     LLSLPLVSQAQTKTIISGKIRDVNGEALIGATVIERDKDNRTLASAITDIDGNFTINM
                     TNTNNSLVFRYVGFQEKIIVPRTQRVITVVLEDSNKTLDEVVITAKVQNSLGGLNIDE
                     RDLSMAVSKISMDELEGVHTSSIDDALQGRMAGVDIVGGTGNPGGGMAIRVRGLTSLN
                     GNNEPLIVVDGIPLETKIGSSSSFDFATATEEEFSQMLNIPPSDIQDIVVLKDAAASA
                     IYGSRGANGVLMITTKRGSISPPKIDVQTTFTLNAPRPTLPTLSGYEYVTLVNEAMFN
                     AGKVLNTATYPELAYDPNNPEYYYNYSNNTDWVGALTKNGYAQDYNISLSGGSKKVRY
                     RFSAGYWDETGTSIGTGFQRLNTRANLNYTVSDKLRFVADIAYSNSTTQSNFIPEASN
                     NMSGDLWSKAYIKMPNQSIYYYNVYGELTSQYFTPYSNIQGKYPNVYNPVAMANDSRV
                     DKGSVSALPKFSLIYDFNPQLQFSFDIGLSINNNKTQLYLPQTASGLEWNNSSTNWAS
                     DRDDDSFTVQTYSKLRWIPTFKDKNKHRLIGLLGLNTYDSRGSSYSESSSNLPSTMLQ
                     DPSIASRVYPSGSIGAGFSQSRQLSLYANLNYTFMDRYIIYGSIRTDGDSKFGKNYRY
                     GIFPAISGRYRISGESFMKEIKWINELSLRASWGLNGNPPNSNYMYYSKYNNYDWNYL
                     GNTATYPASISLDNLHWEVSTQKNIGLNFEGFDNRVNMEFEYYIKTTVDGIGTSALPS
                     TSGFYNTTMNLNTVENRGWEFNIQSTPYRNKSWKVDLSFNVSRSQNYLRKLSEYAGTE
                     SGTWQANGSYLIRNVLNQPYGSFYGYMYNGVYLNQNQTIAIDKKGNPIYTMGQTGEME
                     PVYMRFNYPSIGYIFQPGDARYVDVNKDGNINLQDVVYLGDYNPLFTGGFGPSIRFKN
                     FSLTGWFHFRYGNDVINMTRMNMEKMYSFDNQSKAVLKRFRTPFEPGFEDQAPADLLP
                     RALYAAGYNWLGSDRFVEDGSFLRWKTVTAKYNMPKKLIKGIGLQDLNLWLTMQNLYT
                     WTNYTGQDPEVSLSSNGRDYSRTPRPYQFTMGLKIGF"
     gene            complement(35068..37566)
                     /locus_tag="PKH58_00615"
     CDS             complement(35068..37566)
                     /locus_tag="PKH58_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNX87556.1"
                     /translation="MVNKIKIYFSLLLCLFLFANCNKEGEDYFAEPDWLEKPLYQVLQ
                     NEGHFKSYLACIDRTLYANQLKEGGYFTLMAPNDEAFAAYLSANGYASVNDIPQEEVN
                     KLVAYSILQSYWLSENLGDLFRGSVGDRYDVGDGLKKQTYYYATLYKDPDYNNSWVID
                     QNTNSSTYSQSSYNYKYYPVFMQSFFAKSNLTAQDYNVFFPNTEYVNGQSTETGMIGN
                     VMNGQIVKPNMKARNGIAHEVSVVNLPMENMDKLLQKNEYSAFKSLLDFKDLSGAYVY
                     KNYTEDITLTEKYKILKPNDAIDKVFVKSYNTSGTQPLSFSPALEAIYNDNAVTTLSD
                     GYTLFVPKNEVLTNYINTRLLKYYKSLSELPVEAITTLINTHMANTMIWPSQLKSSQV
                     STGEYVNGEGAKGKTFDEFGVINKKLTSNGFVYTIDHVIKSKLFETVYTEIFLNPAYS
                     LLNTAYIKFYQNSLRESLMKSAITGYPNTRYTVLLLSNELLRNDGLTYNSETTNFSNA
                     LLVGTNVTDRLRRLMQLHLFEGWVDAGVNSEVNFDDGITAYGGWGFRNTTSGDVVRYK
                     NNQLQASGNIEDNTFVNITKGETFDNGTVYTVDRMLQYSNRETSPSTKEGWNSNTLWY
                     YLQQTARENINVTQFVDYIQYALKSTTTDELNGISENNFYTIMMPNNNAITRARANGD
                     LPSLDSLKNGLLAQSRIDIAVNFCKAHFLEGFAFPDDKQPFLYPYNVNSPTMNIVSTC
                     NRYNNEALRFINQRTYVIVSKDASGTLSFTPDNVYLNGKLAISGSFGMTTPAPRVMTG
                     TTKTGYNGYRSNRIAGRAIHHEFTNYFKFKIINP"
     gene            complement(38151..40475)
                     /locus_tag="PKH58_00620"
     CDS             complement(38151..40475)
                     /locus_tag="PKH58_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX87557.1"
                     /translation="MSELFNFGWKFSMGDLKDAEKVQFDDNQWKTLDLPHDFQINQPW
                     DKSANGARGFKRMETAWYRKTFKANPEWKGKKVYVDFEGIMLTGDVWLNGEKVGGTDY
                     GYLGFETDMTDKLRYDSDNVMAVRASTGREGNSRWYTGGGIFRDVHVFVKDSVSIARH
                     GVFVTTPKITTENASVNVQVEVEGIRGKTLNVEILARIFSPDGKQVGETRIFAPQKSK
                     KSTDEVLLPELNVPDPQLWSCETPNLYTAEITLTLDGNIIDKTSEKFGIRTLEFSKEF
                     GFKLNGKKVFLKGIANHHDLGAVGAAAFETAIARQFDVLKSFGFNHVRTSHNPYSEAF
                     MRIADEKGILVVDELYDKWSNKDYWAGREPWTDVWYKHLTEWIKRDRNHPSVIMWSFG
                     NELQMREDLAGFPTGDWGVTTYKILDVLAKRYDPTRKTTVAMFPARAGALTKTDADFN
                     TKIYPPELATVTEISSFNYRWFNYQDYLKHAPDMIIYQSEATTNELAQPFFGMDREKM
                     VGLAYWGAIEYWGESHGWPRKGWNYSYFNHALLPFPQAYLIKSIFSDEPVVHIGVVDK
                     ASESMEWNEQTIGTLNVSDHWNREKGKSYNIFTFTNGDEVELLVNGKSIGIKKNITDD
                     IKKRNMILWQNVPYEAGKITAIARKNGKEVARHQLETTGKAVALKIELENNNFKANGM
                     DLQYVKVYAVDSKGRTVPATTGEVTFDVMGNAKLIAVDNGDHTSDELFDGNKRLLYNG
                     FAMAILRSEKTAGQAKIKVSAPGLKGTEKIIVIK"
     gene            complement(40575..41726)
                     /locus_tag="PKH58_00625"
     CDS             complement(40575..41726)
                     /locus_tag="PKH58_00625"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HNX87558.1"
                     /translation="MKKTFLPVLLMFLVSSYSFSQKPPIMGWSSWNNFRTNIDEKMIR
                     EQADALVSSGMYKVGYRFINIDDGYFGGRDANGNLQIDTKKFPSGMRALTDYIHSRKL
                     KAGIYTDAGESTCGSMYDNDKNGVGAGIYGHIENDCSLFFNDWKFDFLKVDWCGGEKQ
                     KLDEETEYLKIIKVVKAINPNIVFNICRWQFPGEWAIKKADSWRISGDISDKFSSILH
                     IIDLNADLHKYCSPGHYNDMDMLQVGRGMSYDEDKTHFSMWCMMNSPLLAGNDLRTMS
                     RETIEILTNTEIIALNQDKGCKQASRVAKEDDVEIWLKPLGKNGKSKAIALMNRSEKE
                     KTIEVTPERAGLTKNNKLRDLWLHKNLGKFGQSRSFTIPKHGIIVLKAD"
     gene            complement(41771..43087)
                     /locus_tag="PKH58_00630"
     CDS             complement(41771..43087)
                     /locus_tag="PKH58_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HNX87559.1"
                     /translation="MKMNVILLFAVLLSIHTYASDLFPDGTPIPDWFRKTETVDYKTL
                     GNQYVITKYGVKKDSTLLQTRQIQAVIDKAANAGGGVIVIPKGTFLTASLFFKPKTHL
                     HLAEGAVLKGSDDISDFPVLETRIEGETCNYFPALVNADKVDGFTLSGKGTINGNGLR
                     YWKAFWLRRTWNPKCTNKDEMRPRLVYISNSNNVQISGIRMVNSPFWTSHYYKCNNLK
                     ILNVHFFSPVKPVKSPSTDAIDIDVCSNVLVKGCYMEVNDDAIALKGGKGPVSDKDPN
                     NGGNYNIIIEDCTYGFCHSALTCGSESIHDRNVILRRCTLRNPQMLLHLKMRPDTPQK
                     YEYILIEDITGNTGKFISVRPWTQFFDLKGEQAPPPSRSGNITMRNINLECETYFDVA
                     KSEKYVLTDFIFENLNIKANKNSDFDKSIIENLKLNNVKVNGVEIK"
     gene            complement(43181..44083)
                     /locus_tag="PKH58_00635"
     CDS             complement(43181..44083)
                     /locus_tag="PKH58_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNX87560.1"
                     /translation="MKQIRLLFSVVFFCCNFPGFVNARDYKLVWSDEFKADGAPNPAY
                     WSFEKGFVRNNELQWYQEENAYCRNGVLIIEAKRDKIQNPLYSAGQENWRRRREFIEY
                     TSSSINTRDKKEFLYGRFEIRARIPLESGVWPAIWTLGNSMEWPSCGEIDIMEYYRIE
                     GVPHILANVAWGTEARYKPVWNTKAIPYSHFTSKDKKWGRKFHVWRMDWDENAIKLYL
                     DDELLNEISLSETFNGQIGDNANPFRQPHYLLLNLAIGGQHGGEPVINAFPMKYEIDY
                     VRVYQTPEQQNKQQKYFNRLTGDL"
     gene            complement(44166..46619)
                     /locus_tag="PKH58_00640"
     CDS             complement(44166..46619)
                     /locus_tag="PKH58_00640"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNX87561.1"
                     /translation="MKTTAILLLLTICMTLSAGSKPDGYFLKISKTEKTSFESTFKMG
                     SSASPSGPTVLINSSHMLMDGKPVLPVMGEIHFSRVKDTEWKRELLKMKAGGINILST
                     YIFWIHHEEVRGMYNWEGQRNLRRFLEICKELNMPVVLRIGPWAHGEVRNGGFPEWLV
                     NSGLKLRSENKDYLTEVKKWYAEIYRQADGLLWKDGGPVIGIQLENEYRGHGAHLMTL
                     KKMAVELGFDVPLYTRTGWPKLTSPVPYGEIIPLYGDYADGFWDRSVAEMPGDYGKSF
                     LFRSFRSSTVIATEQLPAQSENDASGDTGYPYFTCELGGGMMTSYHRRINIAPMDVYS
                     MALVRVGSGSNLPGYYMYHGGNNPDGQLTTLNERQASNYTNNNDLPEKSYDFQAPLGE
                     FGQINPHYYLLRKLHLFLKDFGGELALMKPYFPENTVTDFKTDTLLRWSVRTNDNSGY
                     IFVNNYQRLKTLSPKNDIQFTLDFPGEKLQIPQKTMSVPSGSAFFIPFNMDLNGAKLV
                     YATAQPVAKIEEAGRTTYVFSEIQGIPVELAIDNYEVSVKSFAGKRRTEGNRIYLSGL
                     KTGTSPAVRLRTASNREISIILLNQKTSLKLWKGKFAGKERIFISNADLTYDGNRLEL
                     VRDKSDCEVFIYPALNSLEYNGKEVKPSSDGIFSKYKIKLPEIRRIKAGLTKIKDMDL
                     PLRVVSFGTAKVAEMPRDEDFTKAAEWKITLPEYDLSLRNLTLRITYKGDVARVYAGD
                     TLLTDNFYNGKPMDINLGYFADKIFGNDLKLSILPLIKNEPVYFQQQAGITFKGADYL
                     LETPTIEVIESRSAVLTAK"
     gene            complement(46631..49294)
                     /locus_tag="PKH58_00645"
     CDS             complement(46631..49294)
                     /locus_tag="PKH58_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNX87562.1"
                     /translation="MKKIWILALFVLCAFTTVVAQDRSLYRKYAPFRKIKIACVGNSI
                     TEGACASDRVNKGYVGVLRDLLGDAYEVVNFGVSGATACRGTYKPYEGLEAFTKAKDL
                     QPDIVTIALGTNDSQPAVWNTGTFAANFEKDLSWLCDQFLGLPSKPAIYLCLPIPVVP
                     NTRWKHQLKVIADEIIPIIRRMAKNKGLRIIDLHTPLTGKPEYYADFLHPNDAGHQVI
                     ADLIYNALKPGYDFSKLKREKLNRGLVAVRENDSTVNISWRYLSTDQTGTTFDVYRNG
                     QKINNMPVSNTTYYKDLKAAPGKLIYEIKASNTTDKTINEICSFEIPENSSDYINIPL
                     DVPPGNISLTGQPYFYSANDASVGDVDGDGEYEIILKWDPSNAHDNAHDGYTGNVYFD
                     CYKLNGKKLWRIDLGKNIRAGAHYTQFMVYDLDGDKKAEMVVKTCDGTVDGQGNVIGD
                     KNADHRNEAGRILTGPEYLTVFEGLTGKALQTIDYIPPRGNLSNWGDVEANRSDRFLA
                     GVAYLDGEYPSVVMCRGYYTRSVLAAFDWRNGKLTSRWVFDTDTPGNEAYTGQGNHNL
                     RIGDVDGDGCDEIIYGACVIDNTGKGLYSTGMGHGDALHMTAFDPLSRKLQVWDCHEN
                     RRDGSSFRDAETGKIIFQVKSDIDVGRCMAADIDPNHKGVEMWSIASEGIRNIKGEVI
                     NPTIRGVSINMACWWDGDLSRELLDGTRISKYYPETGLAELLFNNNDCVRNNGTKSNP
                     CLQADIFGDWREEVIYRTRDSKNLRIYLTPFETQYRFHSFTEDPVYRISVATQNVAYN
                     QPTQPGFYFGSDLGKSFPQKELVVNDDHVMLDAGVIYDNYCWSVGGNEHLRYLTTKDI
                     PAGTRTKVEVTVTFRGFEFKDYLFVTLNPRK"
BASE COUNT        13099 a        11099 c         9948 g        15300 t
ORIGIN      
        1 attttttatg tatatagtat aaaaaagggg agattatccc ttaatcaacc ttcactggtt
       61 ctgacaataa caggttgatc acgatataca ggtgggcatg atattctgct ttttttatca
      121 caagaaagac tttgcggtat taactaaaca aattttctac aaattgtttg gtttcgttca
      181 ggtgcgttgc aatatagaga acaagcacgt cgacaaaaac aataaatccg gcaatctcat
      241 actgaattaa tctaatttta gttctgcttt tcataacatc aaattttcaa tagattaaca
      301 atgtagcaaa aatatgtaat tatatcacaa taaacaaatt attagatgtt ttttgaggtc
      361 aagaaaattt taaatactca cttaagagta gaatcgtctc tgaacacaag gtgaagctat
      421 cataatggta tttatactgt atcctcctgt ttattttgtt agctgattga aaacactttc
      481 ggtgactgta aaggccgttc cgtgacgtat gcctttggga aatgttacag aatcggacac
      541 atcctcccaa ttaatcaaat ccgaagaacg gacagcgcca tactttttat cgcggtactt
      601 gtcgaaataa acgtacacgt aatctcccac ctgcagggat gcaggacctt ccgcccagta
      661 attgccggtg atgggggctg aaaccttgtt gggaaatctt aatggtttgt tgtttgaaac
      721 cacccgtata tttttctcgg ccgggttcga attctcgttt tttacaaaca tataataccg
      781 gttgttacgc tccatcattg tcccgtcaat aacactgaaa ccgggattgt aaaacaaccg
      841 tgtttcagag aatgtattga aatctttggt ggtaacgtag taaagcttat ggttcagccc
      901 tttctcactg gtcgaagtgg gtatttcggg gaacgctccg ggaacggttg atgcccagaa
      961 gatgtagaag agtttatctt tcctgtcgta aaacaattcg ggagcccagg tgttttttac
     1021 tccagcgaat tcttccatca caggaatgta tttttgttct gaccagtatt tcagatcctt
     1081 cgaggaagca tacccgatgc cgttgtccca ccatcccgat gtccacacca tgtgaaatac
     1141 accgttgtcg tcctgaacaa tgctcgggtc acgcatcagt ttgtcttttc ccactacagg
     1201 tttcaaaaag acagtatctc tgccgagcgc ttcccacttc aacccatcgt aactataggc
     1261 caaatgcagt ccatcgccgt tgcctttgaa ataagagaac agataaacgt atttatttcg
     1321 cacaggtaat attgatccga tgccctgatc ttcaacgccc tgtttgatgt gattacacag
     1381 tccttttaac ttcagttctt ttattccttc ggccacgaaa tatgccacct gttccgctcc
     1441 ttttttcatc atatgtgtat catccttaac tccatcgggc ttagccgggt cttcgccggg
     1501 ctctgagtga cgatagtaag caacagattt tttatcacct aatacagtta taaaatcccg
     1561 ggttttctga ttgatgtcaa tgagtggcac attgaattcc cgggcaatct gacgcataat
     1621 aacaggatac tctttcaggc ggtcgtcggt aaggttgccg tttacaaaag tgcgcataac
     1681 caccgaagtc aataaaaccg gatgagcctt tttatctctc acatcctgta tcatcttcag
     1741 gaggttatcg cggtagtcgg cacgtgaagc gtgccgttcc ggacgtgtgg aggcatcgtt
     1801 gtgcccaaac tgtataaaaa cgtaatcgcc tttacgtacc tgtgccatca gtttatccca
     1861 gcgtccttcg ttgaggaaag tctttgtgct gcgtccgccc atggcgtgat tgaccacttc
     1921 aacgttatcg tccagaaatt caggaagcat ctgtccccag cctttaatca gtccgtcctt
     1981 ttcagttttg taggtctgcg cggtagagtc accagcaatg aaaatcttag tctttttatc
     2041 tgatgatgta aaacctgtta cgagtacaag gataatgagt gatagaatgt attttttcat
     2101 aaaatgtaag cggcccccct aaatcccctc tggggggact tttggtgagt gcagttaagt
     2161 gattattttt atattctgac ggtagttatc accatcaaga gttttgtttg aaatttacct
     2221 ttcatatcta agcccctgaa tggtaactgg tcccaacaat ccggaaggtg ctgtttccca
     2281 tttatcatag agtgttttct tataattgat gtccacaaaa tttatttcgt tgaaaatccg
     2341 ccattccact ccccgtctgt cgtaatcgcg aatgctgtta gcaggtaggt tggtgacctc
     2401 cacttccagc aggttttcgc cctgtttcag gtatttcccg atattggttc tgaacggcac
     2461 cgcgaataag gttgccacct cattaccgtt tagataaaca cgggcgcttt cgcgtacatc
     2521 acccaggctg agccgatatt cgtcagccga caggtcattc cagataaatc ttgtactgta
     2581 tcgtgccgtt cccctgtttt tcgttgcatt ttcattcgcc agttctgtcc acgaaatcag
     2641 tttacctgtt ttgaaaactc ccggtatagc cggttcgctc tccttaaaag taatttccca
     2701 gttatcgggc agtgtcattt tctgaccatc gggtatgaaa tagtgccagt tttcagcttc
     2761 catgttttta tccgtgaaag ttttaagaat aattgattgt cccggctcaa gttccatata
     2821 cacttcggtg gcgttattgg ctgtacgtat tttcgccttg cccgaagtac cggtcattgg
     2881 atcgaagaac agggctgact gtgcatgtac tgccagcgga acccagtttt tcaccggatt
     2941 gtttgtaagc tgcgtaaaga aataatgata tcctgtttcg tttttacggc ggatgtattg
     3001 ccccttaaag ctttgtgaaa agctttcact gtatataccg gacatcgtca aaagctcatt
     3061 gaagtcattc cctgtgataa tatttccatt tccccatttg ctcatctctg acttagcgaa
     3121 gttcgcttcg ggcagtgtat taatcatctt tttcatcctg ttctcacgtt ttcggtaatc
     3181 tttcagtccg ggaacacttt cgggataatg ttctgtaaaa attatttttg caccttctct
     3241 ggcaagatgg gataatttct ctaaagtttc cggcggcata aatctcgttg ccgggagaac
     3301 caatgcttta taagttgtcc caccgattgt tttaatcaaa ccgttttcga cggtggctga
     3361 catgatgtaa cggtcggtaa tgtaatccac gtcccttccg ctttccctga ttttctcaac
     3421 cgtattgtag aatttcggta gccgtgcccg caatccgtga atgccaaagg caaagtaata
     3481 gttttcctgc tgctcatacc agatatcgta caccgggaaa tagaccagca aatcgttatc
     3541 gggctgtccg tactgtagga atgactgtac acgggtaatg tacgagaaga aagcgggagc
     3601 atcgcgccag atggaattcg tgggcgacat attaaccgag gcatagaata gccatccggg
     3661 ccacgcagct tcgcggggcg aatagggagt cccatgaaaa aacacgtgat tgacacccga
     3721 tgtaaagacc tggtcaattt ccggtttgca ttgcgaaagc gaagttctga aatgttcggt
     3781 aagccaggta aatgtttcgg acgaagtaaa ctttttaccg gttatatgtg ccgcggagct
     3841 tgccatttta agtgtaacgg gatctccatc gtttattttc ctcatttcat cttttctcag
     3901 tcccggtatg tcgaagtccg ttataccgaa gatttcacat tcagggatat cgacagcagc
     3961 atacaaatca atgatattgg caggtgatcc gtgtgcctga ttgcgtgtag tagctccgtg
     4021 actatgcgcc cacgaagtcc actgcgcggt aaaatcagtg agcagcattt cgccaagcgt
     4081 ttcccggtaa tcggatacta cgcggcagga gatgtccgtt tttccttttc cgagtaattc
     4141 cgggaaaaaa tcttcgagtt tgtacccccg tcgctgttga aactcctcca ggaatgttgg
     4201 tgtccagtcg gcattataca cctcgtagga gtcgttgaag aatgtattgg ggcagggagt
     4261 attatcacgg gcaaaagcgc tgtcaaattt agtcaggtaa ttcttcactg cttttgcgct
     4321 gaaatgatcc atcacatacc cctgtccgcc cggagcagag cgtttcacct cctgcagggt
     4381 tttacccaca aataaggcaa tcagttgcca ctcgcctttg ggcaatttac agttaagatt
     4441 accctctttc gatacaagtt ttgtcaaatc cagtttcttt cctgtagccg catacgccat
     4501 cagccgcgac agataagcca cttttcgttg tttggggtcc tccacagtaa tgttcaaaga
     4561 gatatttttt cctccgctca cctcgtattt ttgaaaaaca gcttttgtag cagcatcctc
     4621 caacgtaact tcaggtccac cgaagggcca gccggttccg gtgttcatgt ccaccttcat
     4681 gcctgaattt cgcgcttcgt tttcggtatg cgccagcatt tccatccagc ggggagtgag
     4741 atagctgatg ttgtttgcat cattgccttt cactccgtaa atgggtgtaa tttccacacc
     4801 gcctaatccg gctttaccca atttgctaat gttgtagcta agattatcct tatcaacagc
     4861 acttcccagc caccaccagc gcgtccaggg tttgctttca gaggtaacgg caggccagcc
     4921 ggtgtccgat gccattgcat ggattgcggc aaaaaacatg atgattaaca agcaacaggt
     4981 tttctttttc attgctgtat ttttattttg tagaataaat ttcattttct ctttcatcgc
     5041 cttgaaaggc ggggcaattc ataaaatgca aagattattt tacaataaat caataattta
     5101 aaacgtgtcc cgaacttgtt tcggaaacca ataaccaaat ccactatttg atattctttt
     5161 tcagctcgtc gggtgcatcc tttgtcatcg acatatccag ccactcgccc cgctttatcg
     5221 ccagattttc tatcatttcc aaagttacat cattttcagg aaagaataaa ccggagttca
     5281 tatatttcaa aatggaatac cgcaactgtc tggcaacata ccgtatatcc aaatctttct
     5341 gaatatcggc gctgcacacc actatttttc cctttccgac tttcgcttcg aaaagcatgg
     5401 ccaaccggcg gttgataaac caggtgtcga tgggctgaac aatgggctga aaggatacag
     5461 ggaaatcggc aagttccatc acctgtgctc tgttaaccag ctcccaccat tgcagattgg
     5521 agtaaaaatc cgtcgggaag tccttaaata ccggatgaaa atcgttaatc agtatgcccg
     5581 tagtatgtgg cggacgcatt ttaaaccagg aagtattcca gaatgcagga acaaattgtt
     5641 gaaccacatc cttaccttgt gtgattttac ccgccatttg aagaagtact gaacctcctt
     5701 caataagcac ttgttttgct ttatcatcaa ttttatcaca gatataaaca gaagtggtat
     5761 cgaccagggg aagcgtctcg ggatagaccc agaaatccca gttattggaa atgtctgttc
     5821 ccttcagctg tacctccagg ttcagttttt cagcttcggg aattgagtgt agcggcagag
     5881 taatttcgcc taattgctga cagttgccga tttgaatgtc tcttggggca aacgagcctt
     5941 cgtagacgat ttttcccaat tcatttctaa ctttccattc tgtttgtttt cctgtgagcg
     6001 tttcttctcc aaagtgtgct atctctattc ccgcttttag gtcttcatta cttttaaaaa
     6061 taaacttatc catacgcgaa tgcaaaaccg ttggtgcaca aaactttcgg aattcttcgg
     6121 cgttaatata tcccttattt tcccagaagg cattcaacac acctaccagt gccgttccct
     6181 gacctgaata atcattgaga gataggagct gaaatccggc atatccgggc gttcgcaatg
     6241 ttttctcaat ctcgtgcttg taacaaagcg cctgaagttt tcccgatgcc atcagaaaat
     6301 cggctgcctg atagcccatt ccccggtcag tcagatcctc acgaaacaat tcgaaattac
     6361 gggctttcat aatgccggta tatttactta tttccctgaa atcgggaaat acgcaccact
     6421 gaccggtttc gtgcgatacg taaggctgcc gcactgtgtc aatcctgcca cggtaatcgg
     6481 aattggactc aggacgccga tcccagtcca gaccgcgtgc accggctttt acgtgatact
     6541 ggctggctgg ctgccatgcc cagctgccac caaccgatgc tccggtgtac acccttctcg
     6601 ggtctttctc tttccagtaa tttacaaatt tcgaaaccca gggcacccat cgtccgcggg
     6661 gttcgtttcc tatggcaaac atgcaaaacg aaggataatt gccgtattgt ttcacaatcc
     6721 tttcggcttc ctcccaaagg tatttatcca ccggacggcc atcgccaagc gatgatccgt
     6781 ggttaggcca gctcggacct tcgggctgga gataaaatcc aaccaaatca gcggctttca
     6841 tggctgcctc cggaggatta taggagtgaa agcgcatgtg gttaagaccg taactacgac
     6901 agatgcgaaa aacacgttcc catgatttct cgtccatcgg ggcatagcct gtcaggggga
     6961 atacacagtt ctcgactgtt ccccgaagta cagttttctg tccgttaaca taaaaatatt
     7021 ttccctgaat agtaaactcc cgcattccaa atcgtgtatc acgcgtatcg gttccctgtt
     7081 tggtctttag tgtgaccgta agtttgtata gattggggtg aaactcattc caggtcagaa
     7141 aatcatcacc cattggcaat tctatctcca tttttccgac cgaattcttt aatgacagtt
     7201 ctttggatac cggagtaatc ttatggattt tgtcagaatt aaaactttcg gcacttaagg
     7261 taataatacc cgataatttt ccctctatat ttagtcttac aacagccttt ttatttttca
     7321 aatcgggaaa tacctgtatg tcctcaatat atgtctgtgg catcgcctga agctctatac
     7381 gccccaccac accgttccag ttgccttgtg tttggtcggt gatgctgtgc gaatccttcc
     7441 ctacatcgat gtcctttgtc cggttgtcga tgcaaagtgt aatcctgttt ttacctttct
     7501 tcaggtattt actgatttca aactgatgag gaaccgaaag ggagttctgc atacccactt
     7561 ccgtttcatt gatccagagg cgcgtttcag tatgcggacg ttcaaggaac agcactatgc
     7621 gactgttatt ccagtcggca gggataaata tatccctctg ataccaggca gctcctgcgt
     7681 agtatttcac cggagtaagc cagaagggca gtttaaggtt atcgggttgg cgatattttt
     7741 ttaatctcgg attgtagaaa taagtgctgt cgtagatgct tgcagtccac tgggtgttga
     7801 gcgttacatc atcgcctttg cggttttcag tcatggaacc gggcagataa atattgtccc
     7861 gcaacgtttt tgagaaccat ttttctgaca cacccacatt gtttctgtcg atatcgaaac
     7921 gccatttccc cgataaatcg atggtgagat tttgcccgga agcacaaagc gagaaaaatg
     7981 ccgcaagtat aaatattccg cgtaatttga aagttgtcat agatcgtttc atgatattta
     8041 tatatttcgc tgattaattg tttgagagaa aaacctgctt tgcaccataa gctggtgtca
     8101 gtgggaaaac atagaatttc ctgttctttt tgaaatcaat catccactga tcaatcagta
     8161 cgtgattatc gagggcggta attgtcaacg tatgtttccc tttcaggagt gtatatccgg
     8221 tcgttttcac ggcctgagct ctcagtacat tgttcttcca ggtttccgtt cgtcctcttt
     8281 ccctgaatga aataattttt ggtttttcgt catctatctg cacactaaac cggatatcgc
     8341 ccttgtcgtt gggttgggtc gggatgacag cagtgcgtaa cacggcttca ccttcccagg
     8401 ggcaatcgaa ctcaaaagaa accgattcgc tacggggcag agaaacggca ttcatactgt
     8461 gtcccagcat ctgaatggat tttacctgct taccggcacg ggtaaagtgg caggcattac
     8521 gggcaatgaa actgtcgggt tcaagctgat caattttagt tgcatgatac gacaaaggca
     8581 ggtcatattt tctttccgta tcattcccgt ttgttttcaa cgggagcacg ggcgcatcaa
     8641 aaacgggtaa atcccgcgga gcggcatgca tggagtgttt ccattttccc ccggacatcc
     8701 ggttgttcca gtaatcggta agtgtctgaa tttcagcata cgcattcagg cttcgggtac
     8761 atgcttccac taatttcgtg ttattttgcc agtgaatggt atccttatcc tgcaaagcaa
     8821 tgtatcgtgc ccgttgagcc tcaagccact tggctgccat agccgaagcg ccaaatacgg
     8881 gatattttat ggcagcaaag aaagcatatt tgcggttttc agaaatcaga ctctctgtct
     8941 gctgaacaat atttcttatc tgttcatagt cggaaagata ccggtccagt tcaccgccaa
     9001 attcggttaa actgaattca gtatcgatga ctggtgtcag tcctctgttg tactttttct
     9061 tgtcttcttc cacttttgtc catcccatga attcggggcg gcggatattg ctcaacctgt
     9121 aatattcagt cattgcaggg agaagtttta ttccggcttc acggccaaat tcgcgacaaa
     9181 gccatttttc cagatgagtt acgattgatg tgttttttat gctgttgata ttccaggcca
     9241 tatccaggaa aaactccagg tcatatattg ccggtttcaa atcgtgtaca ttcaccaccc
     9301 agaaacgtcg agcgttgtgt ttgtaggctt ccatcatctc gttataaatc aatcccggct
     9361 gtgtggtcgt cagccaaagg taatcatgcg gacgacccca atagctgagg tggtaataga
     9421 taccgccacc accactacgt ttctgttgtt gttcgtcact caggcgtgtg atgtatccat
     9481 agttatcgtc acaccaggta agcatcacat catctggcac ttttagtccg ttttcataga
     9541 tatccaacac ctctttataa ggcacaaatt gctgcggaac ctttgacaaa tccggattta
     9601 catgttttct gagcaattcg cgttgttcgt tgattacttg ctgcaaagct tcggtttttt
     9661 ccttcagggt ggaaacgcct tccatggggc catcgtgtac accacgcatc ccgatggtat
     9721 aaatattttc atactgatga acttcattca gtcgttcttt ccagtaactg ataacccggt
     9781 cgtgattatc cggaaaattg aagttaccac ggattttttc atcccactct cctacgttgt
     9841 tgcgcatcag cggctcacag tgggatgttc cgataacaat accacaactg tctgcagctt
     9901 ctttggcacc gggaacaaaa tagaatggca cagtggcacc atgcatagcc ggccagatcg
     9961 tgtttgcacg caagcgcagc agtaacttga aaatctcacg gtatgttttg gccgaaatta
    10021 atcctttccg gtcagaaggg tcaaagtttt tccagctcca gagttgcagc gaccaatctt
    10081 cgtcattgat aaaaatgccg cgatattcga ccgagggcga ctgaagagtg tgaaaatcct
    10141 ccggtacagc cagtctgttt ttcttgtcgg gtttcatgtc gccccaccaa atccagggtg
    10201 aaacacctgc aagccgcgat aattcaagta ttccataggc tgttccacgc gcatcactgc
    10261 caaccacaat caactgttta tctgatactt taataaagaa agcatcccgc ttccccgcga
    10321 gttcatcaac cggaatccct acatttttca gttgtttact gagtttttct tcaaattgaa
    10381 caattcttat tgtagccagg ttttccggct tttgctgagg tgcaaatccc gtaacctgga
    10441 gcatatcatc acaaaacatc ccggctgcca cttttactac cggagctgcc tgacggggta
    10501 aactgtagct aatcggatta ctgccatcga accataccaa tccggtcgcg cttgtctgac
    10561 tgcagaggaa aagcacgaaa agaaaaatgc ataatctgac ttttttcatt ttttttgaca
    10621 gaaggataat tgcgcaacct gaaaagtttt ctgttattta ttttctattg attgattcca
    10681 aggggtaagt agccaaaaca tttgttattg tcttttctta caggatggag atggcataca
    10741 accttcaaaa aattcaattc cggattaatt gtccgaaact gaatttttga aatatgaatt
    10801 tctacttttt atcgatactc agaataaatt attttttgat tagcttatac gttgctgtag
    10861 aaccagaatt tgttaaccgt aaaaaataag ctcccggttg cagatgagaa acgttcattc
    10921 tgaccagact attgtaccca ctttctgtat agctgagttt tttcactcca ttaacaccgt
    10981 acaagtcaat ctgcacctta cctgtaatgt ttcccaggtt aatcagacaa tatttatcca
    11041 ttactgccgg ataaacactt actgaggagg cattgatttc gttcaccggt gtcggatcat
    11101 tgattaccgc tattattccc tgattcaatc tggtcaggtc ccagatcaaa tcttttccgg
    11161 ggattgcagg tgtaatggaa gtaaatgatc cgctgatggt tggagcaaca aacagggtgt
    11221 agctttttcc ggcagtccag tttcccgaaa gatttcttaa ttccagatta ccattaagat
    11281 aaagtgttcc atccacttta agctggtcgt ttgctgttga aatcgttttc ataaccaccg
    11341 tggaaccaac ggtcagcgag aggtttttgc tgaaagtaag agttccgaga gcagtttcat
    11401 tgttaccggg catcactttc gccgaattac ttatgttggc attcccgccg attattccgg
    11461 ttccagccag gatagccgat ccggacatgc tcagattacc ggttccggta gcgctgccag
    11521 tagtattctg aacaacaagc gttccgtcgg taacagagaa tgtactggtc agtgtcgaag
    11581 cattggtcag cgtccatttt cccgttcccg atttggtaac ggtagctccg ctgccaaata
    11641 ctcccctgaa aattgcatcg cggttgtccg ttccgatctg ccaggtaccg cctcctacgc
    11701 tcgaagtggc atcaccagcc agtgaaccga ttttgactac tgtagaagca ttggtgctgc
    11761 tggatcctat tgttgaatat gcacccataa acacattagc tccgagattg atagctgcct
    11821 ttgggaaagt tgccgcatta gtggtaattc tgaaatcccg tcctgttaca ttgatagttc
    11881 cggtaaagtt atttccacct ccagctacca aatctgcacg tacatagggt acgtaataat
    11941 ttagtgtacc cgcccctgaa atggatccgt tgatgtcgca acggctggaa gcgtcaattc
    12001 gtgccgtact accttcagga atggtgatgt accaggtagc tttctggttg taatcgctgt
    12061 tgttgtcgaa catgcgtatg cgggatccat tttccatgcg gattttacca cttttaccaa
    12121 atccggtccg gttcatttcc aaggttccga gaccaatagt tcctgcttta atgacagttt
    12181 caccagtata cgtgttggga gaactgcttg agacattcag ctgacccgca cccgatttaa
    12241 ccaaaccacc tgttccggtg attgtaccgg tgattgacac tactccgttt gattttggaa
    12301 tctcgatggt gtcagttccc tgaatggtca atcccctgtc tgtgtttgat tctacagcat
    12361 ccacaataag ccgggtatcc gtcattaccc agtttgctgg agcagttgcc gcctgaccca
    12421 gcggtccggg catggaggtc tgattgagcg cgctgacctt cacctgcgcg ttgttgtaaa
    12481 tcgtttttcc ggtaaaggtg ctttgaatat ttcccagatc aagcagacct tttccggatt
    12541 tttgaaaatt gccggaaccg ccgataccac cagtgcctgt gaatgtatat gattttgtat
    12601 tatttgcaaa ctgaacgtct tttgtaatca tagattcgga gagcgataca ttggtctgaa
    12661 ccgcgctgtc gtcaaacaac acactatcgt tctgaacaaa gaaagtggac tgatgtggca
    12721 aagtaatcgt ctggaaattg gttgtcatgt aatcccagtt cgcatctgta gatccggtcc
    12781 aggtaacacc ggatgcagag cgtgtagctc ccacaaccaa ctgcaagtcg tggctattaa
    12841 tcaccaggcg aacaggtaat ccggcaactc cggtcacatc gaaattcgcc agtgttcctt
    12901 ttaatgttcc cgtccaggca accagcgtat aagttccggg ggtaagggta ccatttacaa
    12961 taatagtgtt aatgccgttt gcagtaaaat tcccgttgat gaccagcgag tcgtttttat
    13021 acggatcttc gggaattatg tcaatttcga tgtttgattt gccaggtaat gttaatgctc
    13081 cattgataac aattttacca agtttgccgg cttccaatcc gttccccggc gagagtcttc
    13141 cgccttccac atttaacccc ggattgagca cgatgccgcc attgaggaca gcattcccgc
    13201 ttaatgttcc ttttgctctg acatctatcg gactgttgtt gctgccgttc aaacacaaat
    13261 ttccttctga aataatagtt tttcctgtat aggtatgatt tccgttcagg gtgaatttcc
    13321 cgttttgcga ttttacaagt tccattgttc cggtgagttt tcctgttcca cccagtacat
    13381 aatcctttcc tttcgggttc atcgcccata tttttgaggg agccagatct gtggtgagtt
    13441 gaaccggagc tgagttatca cccgagatat cgaacatcac atcatcgccg tcaacaaatg
    13501 ccgttctggt gatttcgtcc tgctggagaa aacttgttga tgatttgtca aatgcagttc
    13561 ccgacgacca ggtaagtttt gctttctgta caggaggcac aggtggtaca ggcatgttat
    13621 atcccaggta gaaatcagga ataggagact gataatatcc tttggtagta gcctgatcac
    13681 gataggcagg attttgcatc aggcagtaaa ttctttttgt tgtgggataa tcggtggaga
    13741 aaccaacaaa ccctgtattg gttgtatatg ttgagtctcc aacaacctga ctgcctttct
    13801 tttcgagcag tacttcttcg cgccagtcgc cgataatatc gccaaaaaat gcaggacgca
    13861 cgccatattc cgaattcacc tgccaacctg tcatttttgc aaattctatc aatcggtttc
    13921 cgaatgaatg tgagctaaca ctgtattttc caacatgagc attaaaacca ttcccgtcag
    13981 cagaagacag ttcttctctg tcgagttcac catcccacca aattccttcg tgaggaaatg
    14041 gtgtaccgcc ttcgtaaaga atattacctt ttgcatcgta gatattggcc atggttgacc
    14101 agaattcata gcccagacgt gtagagtcca cgtccataca ttcaccacga ccaacatcgc
    14161 ctaccgctga catatagatt ttttttattg cttcaccggt accggcatcg taaatcagct
    14221 gccccagcat ggtgggattg ttttgctgaa tagcgaatgt ttccagtccg ggtctgtcgg
    14281 gatcgatatc gccggtgcgg aaccggtcgc cgtggcttat attagcattg aaagcgagtg
    14341 tgccatcata ttttatgcca taaccgccat tcagcaattc atcacggcca tctaaatcca
    14401 catcagcaga tctaatacta tggaattcag cagcacccac ataaccacgt tcccagttat
    14461 accagtttac ccattgtccg gaagcattgt atccataagc tgatgcataa taccaatgat
    14521 acttatctga acttgtacgg gttttgtaaa caaatccaac acttgggtgc ttgccatcaa
    14581 tataaatgat tgccatcata ccattcagat tggcatattc cgttcccata aaaatagcct
    14641 tattggtgcg ggtataattg gaaggatatt tcatctcaat actgttcttc tcttttccgg
    14701 tcattccatc aattacagtg atatattgcg gtgggttttt aacgttttgc gaattgtaat
    14761 caatgattcc gtcgttgtct gtatcgagtg tcgtcgatcc gttcacacct ttaccatcag
    14821 caaattttgt cccgtcgctg cttttgatta ctacgtctgc ttttccatcg ctatccatat
    14881 cataagcgat taccatatcg tcctgaccct gagatataga aacattgggt cccatatcca
    14941 cggtccacag atactgtccg ttgcgaagat agccctgtat tttatgtgta cctccatcta
    15001 cagacagtct gtcaaccaca aaatcgtact caccatctcc atcgaggtcg gcaggccaaa
    15061 tgaatttggt ggaatattta tataaatcaa gtccgtcagc cagggggctg tagttgatgt
    15121 caatataagc gctgcggtaa gttgcgggaa aaccagtata gggtattttg aaaattccgc
    15181 tctgtgtacc ctccacgcca ttaataaccg gagtaacata aagctgcgta tttacgccta
    15241 tcgcaccgga agcagcggag taattggtgg ctgtaagtgg tgaggcattc agtttcacgt
    15301 atcccgaacc tccggatacc cttgcataca aattgaacag aatgtcctcc ggatcagtgg
    15361 cgagatatcg ccagctaacg aggtacccgc cactgctgtt tttcacagca acaacgcccc
    15421 tgtccagttt ttccatcact cgttgtgcat aaacacccga ttgcagtacg ccagttatta
    15481 ttaaaagaaa cagaactaac gactttttaa gacctaaagt tttcatattg tacgatttct
    15541 atagtcggac ttttcatcca atgggttata aaataatgtt ccctgcagga agtttaaact
    15601 tctccctcac agggaacata tttcatttta ataataaaga aagtttattt ggtaatgaat
    15661 gttttataaa cacccgaagt accatccgta tatttcactt taacaaatac cattcctttt
    15721 tgatagccgg atacttttct tccggtcata tcgaagtatt ccatcgagtc aattgactta
    15781 gaataatcga ttgttgatac ggctgtatca tcaccgcctt tataggtcag agtaccggtg
    15841 gcaaaatcga atgaccagtt agttgatggc agttcaatag agtcaaatcc aaattcgccg
    15901 gattcaatgt aattagcagc atccagaatt ttataatcac cgggtgcagg acgttgtcct
    15961 gttgccctta caatgagttt accaccataa ttcaggtcgc cttcaatggt tatcttatcc
    16021 actccgcttg cagtcgcatt gatgatagta gttgcaccac ctgctcccag atcctgtttt
    16081 aaggatagtg ttccacccag ggttaagtta cctgtaattg taccatttac agtgttttga
    16141 atagctccaa tatatccgtt accagcaaga gttgcagtat cagctaccaa aacttcagca
    16201 attttagtta atgttatgga tgttggcaca cctgtagcag taggtataac agtatatact
    16261 ttggccaaac catcgcataa tacgttatta accaccagag ttccatcgag tactttcaca
    16321 ttaccgtaga agtatggact ggctccggaa agcgtccatg tacctttacc ggtttttgcg
    16381 atattataag tagtgtttcc tgcccatgca tcaatattga taggtgctaa ttcacctgca
    16441 aattcggcat tggtattcaa tccgcccact gtataggtca ttgtacgtac atttggtccg
    16501 actaatttcg attttgcagt acctgtaagt tcaccaatca ggatattagt tgttccacct
    16561 gtagctggat tgacagaata aaccattcca atagaatctc ccaaatgaat ccggttattc
    16621 ttataattaa gggtagttcc aaagtaggtt gtactatctc cgcgtgctga gattacattg
    16681 atttttgcca gtgaatccaa cgcgaagttc attctcggca ttttgttcat ggctgctata
    16741 ttgcctttca ccggcaacga ataataaatt tcacttacgc cctttacagt tgaattgtac
    16801 tcaacctgtg cggttggcat tacagtaaat attccagttc cggtaatcgg tgtgctgaat
    16861 acagttgaac caatatttac attcagtgtc gtaccacttt tcacattgta cttatttgta
    16921 acatagtaat tttttacctg caacgttcct ttgttcaggt tcaaaccacc ggtcagctta
    16981 ctcatgaagc taagcgtggt tgatgcattt acatttattg caccggttcc aaacaataca
    17041 cccgtaccgg atatagtata atcgggaaca tctatcgaca tactattggc acccagatca
    17101 aatttagaac tgaaacttac atcttttatt gcagcactgg taagcgagaa ttttacacca
    17161 ttaccattct gataggccag ctggttgaat aagttagtag taaagttagc atccagttcg
    17221 ttccaatatt cgctggtaaa tcctttccac gtatatgtac ctgaagtctg tggagttttt
    17281 ttaaagacag ctgtcaacgt agatccgccc aattgaacga ttacagagtc aggaccggtg
    17341 gctgcagcac tgtaggcata gtaatttcca cccgatgtaa acgatgcttt ttcggattcc
    17401 aataacctgt acatcaaacc gacttcctgg ctggtaagta ctttagaagg ttttgcttcg
    17461 ttaccatcct gatcgatata tttgattgtc aaatcggctt tacccagtga caatactttc
    17521 tgatacactt taaaattatc gacggtagca tctaagtctg catttaaatg gttagctata
    17581 tttgaagctc tgttattcaa cattccaaac tgaacaaaat cggacacggt tggagataca
    17641 aaatccagat tggtaaatgt ttgcgtgttg gctatactat ctttctgcgt gatagtaagg
    17701 tccattttct tagttgcaaa gttgagtttg aagttcagat tataccattt attctttaag
    17761 gcacctacat ccgtcttcgt actcagattt aaagagtccg ttttaaaagt agttgttcca
    17821 gccctcctaa agctcggata ttgaccagtg acaaataccg gaataattgt atccggttta
    17881 ttaacaggta ccggaccttt aatatccttg ttccaaagat ggaatttacc ggaaccgcct
    17941 acccagaaaa gacctgcaat aacatctgta taaggtgtac cagtgttcca caaattaaca
    18001 ctcttactgc ctaataaata gaattgaaag ctattaccgt tactaatagc tgacttagca
    18061 acgagcaaat ccaaatccac gtaaatggta gaggaatccg gtgcaagcgg gatttttatt
    18121 tctgcacctc tgttaccact tcccgatccg gtaaatctta ttccatccac cagtgatgtt
    18181 cctccacttc ttccaaagac agcagttgta cttgtagtgt tggctgttag agaactccat
    18241 ccggtaggta aagcaacagc tccatcgaag ttttcattta taagatactt gtcccagtac
    18301 tcctgcgcct ggacaaatga gaccgttaac aacagcccta aaaataaggt aaaaagtttt
    18361 ttcatgataa aatgttttaa tttaattgat taaattaatg atattgatac gattaattga
    18421 cgaataataa tatacaaaac aaaaatatgt ataattatca gaagagatta ttagagaaat
    18481 gatttaatta ttgtagtttt tgataatatt tctgttttga ctatcaaatt aaccaatccg
    18541 aattaaggta aaccagagag taaccacacg ggacaaaaga atttatcggg tcttgttaaa
    18601 gaggcagttt aatgaagcga acagtataat ttctgtcatt acctttgata ttcagaatat
    18661 acatgccact tttaatatct gaaacattat aattccttat ttgtttatcc gaaacgataa
    18721 aactttcatt cgaaagcaat gctcctgtca ggttgtaaat actcaactga tagcttcctg
    18781 cttcatcaaa ccggatatta agctgctctt tcaccgggtt gttcagaagt ttgatacgat
    18841 tttcggctaa attcttaatt cctgttccgt ttttgaatgt aaaaaacaag gtgtcatgaa
    18901 acagataacc tctgaaattc atatcaagtg acacctgata ttccttatca accgtataat
    18961 cagcgctgtt cagcacaatt gttttggtat tctctccggt tgaccatttg taggataacg
    19021 catcaaaaac aggattaacc gtataggaat tgctttcgat attgatgtgg ttttcaggga
    19081 aaatattttc caaatctgag ccgaaataga agcccgtatg tgtaggttga ttataggcta
    19141 cattctgata aacaacactc atccggtaaa caggatcttc gaggaaagta tggaaacgat
    19201 aatcggttgg ataagtggtc atgtaaattc gtatgttttt attgtccgtt gtccgcaaaa
    19261 ccacttcttc tctccagtcg cccaatatat cggcctgaat acagggattt gctttggtgc
    19321 tgttgttgga ggaacattca gcggatgcat cgagcaaggt ggttattacg ttattcacgg
    19381 gatcggtttt ggttactccg gttccgtcct gcatttcacg aagtaaatct ccgtcccacc
    19441 aggctgccat attcatggat acacttgtag tgcttaccac ttcaccactt gctgaagagc
    19501 gtatacctcc tgtgcgtgac gaccaccatt ccatcccgcg gcttcgcgga tccacatcgg
    19561 aacccatacc tctgcccacg tcatcgcctg aaggaactct aaaaataact tccccggttc
    19621 tggcatcacg gagggttgtt ccatctactt tattttcatg tacacccagc acctctaatc
    19681 cgggcctttc gggaataaaa tctgtcagat gaagtgcatc tccgtgacca agtttagtgt
    19741 tatagagacc tttaccattg ttatcgacag tcatcgagcc atatacaatt tcgtcgtaac
    19801 catcaccatc cacatctccc acgcgcaggt tgtggttgcc ctgtccggcg taagccgtat
    19861 tgcccgaacc tgtccacgta tcaaacttcc agcggcgggt caattttcca tccttataat
    19921 cataagctac aagaaccgtt cgcgtgtaat atccgcgaca cattaccaca ctgtgatttt
    19981 cgccacccag gttagctacg catgccagaa acctatccac acggtttccg taattgtcac
    20041 cccaccgtgc tttcagttca tcacctgttt gagttagctt tggaatttcg ttaccattgt
    20101 tccaggtact gtaccggggt ggatcgtatt cggttgttac tatctcggta cctgtcaaac
    20161 cattgaaaac agtgaggaac tccggaccct gcaagatgta accgctgcta ttgcggtaca
    20221 cagcattttc gttgtaattg accgataaat tatatttggc tttgtattca ggaaaatagg
    20281 ctttacccac aggtgtacct gctgcatccc ggcttccttc agcagttttg caaacgactt
    20341 ccgctttacc gtcaccatcc aggtcgtaca ccatgaactg ggtataatga gctccttcgc
    20401 ggatattcac tcccagattg atttcccaga gaaatgtacc gtcaatttta tatgcctgca
    20461 atacaggagc atcggtgatg cctgaattcg cgttatcctg gccactgcct gtccgatgaa
    20521 caacaatttc catttctccg tcaccatcca gatcggcaac cgaagcatcg ttaggtgaat
    20581 acttccaaag gtcgtcaccg gtaacttgct tcatcggtat ctgtatgtaa ttgctgatgg
    20641 caccctcggt gtattttttc agacggtaac ctgctatgct gtcaccttcc tcaccattaa
    20701 caacaggctt caatatatat tgaagagcca ctgaattttt ttcctggtat ttgaaaaaag
    20761 tattgttggt tattggttct gtattgagtt ttgtataagg ctgcaaagta ccgtcgaaat
    20821 tgaccttagc catatacaga ttaaactgta cagcgggatt atcagacgag aggtatctcc
    20881 acgacacact cacgtatttc acattatttt ccgtgctccg gtatgctacc atccccctgc
    20941 caagattctc ctgactatgg agcgtgttga cagaacccgc cgaaatcatc aacataataa
    21001 caagtaatct gaatgcgggg agcagaaaat gtaacaaatc aactccccta acccccaaaa
    21061 aggatgttct ggaagaatga ttgttatttt cctgttttag gaaaaaggat gaagacactt
    21121 tattaatttt taaatttact gaaaatccgg atttaaatcc ttattgctta aaaatctatt
    21181 tatataacgg taaataccag ttggcttccg acgagaagtc aggtacggga agtcctgcaa
    21241 gttcgtattt gcggtgcatt ttgtcgtaca ggaactgtcc gccggtaccg ttgagttttt
    21301 gcagtcggcg tgccacgcgt atgaggtcag tccaacggtg tccttcgaat gccagttcca
    21361 gtccggcttc cttcactatc atattttcga caaatgcaat agaatcctgc ttggtggtaa
    21421 tctgcgacat acttacattt tgcagattgg cacgtccgcg aatacctccg ttatacctcc
    21481 acgcgctacg gaaatatggc tgatcgataa atctgccatc gaaatagtag ggtgcaggat
    21541 agggtcttcc gggtccgtaa ctgctatatt tggtcgaatc gcctctgaaa gtagttccat
    21601 cggtatgtct ccaggtaaat gcagagccaa tgataccgtc attgaccaat gcccaggcca
    21661 gacggggata accactgccg gattcggatc tgttagctgc ttcggcaaat cgcaggtgca
    21721 gcaaaccggc acggtacaaa taccattttc cggcatcgtc gtatggtttg cctgttacat
    21781 cgtaactcag cgagtacttg gcaacagcat aacgtccgtc tgcagttttg gtaactcccc
    21841 ccgaaacgcc ccggcaatca tacggatagc cgtttctctg tcgttcacta ccccacattt
    21901 cgtcgatggc atattttgat ggcttcaggt aatactcacc tttgccttcg gtgccgaaca
    21961 atttgataaa cggataagca ggcgcgaatt tgctgtcgta agacatttcc catatcattt
    22021 cattcttcac atacctgtcg tccgccttca gcttgaacat attaatccag gaattatata
    22081 atttgctgcc atcatcgggt gtatagcggt taaaaagtac cgagaaatag gttacttcac
    22141 cactttgcca ggatctgtcg tcatatatgc gataggttgt actgttggcg gtgacgcctg
    22201 catcttcgtt cttggccaat acctgcctgt aaatggttgc agctttatca tactcattat
    22261 taaagagata caagtcaccc atcagacatt tcttattaat aaaatatgga atcagactta
    22321 ctccgtcgat agttcctgtt attaatttgg aatttttgta gtcctcgagc gttggtaaac
    22381 tctccatgaa gcgtatcagt tcagggataa gctcatcgag gtttttcatg ttttcctgct
    22441 gatcaacaag atccgcgatt ttcactattg gattggtaat gtagggtata gttccaaact
    22501 gaacccccaa ctgatagtac agccagcaac gtatagcacc aatatccgaa taacgctcgt
    22561 tgtactgatc ctgtgttaac tttttctttt cgagcatgat atcaaaatta tacaacgcat
    22621 cgttacaatt ctgaattacc tcatagatgg gagttacatc tgcccaaaag ttatctttat
    22681 ttgccttttg ttgattgata tccatcaaat cgtgaccggc attggtggta acatcgagca
    22741 tatcaccgcg caattcattg agcactacca accggtcggc aatgcccatc acttttccat
    22801 aaatacccag tacggctgcg tcggcatcat ccagtgtgga ataaaagtct ttgtattcaa
    22861 ccgcttcttc aatatcggct tcgatacagg aactaagtcc catcaccatt gtcagtccta
    22921 acgagataat aatatatttg aaagtattca taacctaatt tttttttgat ttaataatct
    22981 tgctttttac ctgttctttt acaaatcaag ttttactcct actgacagtg ttctggagaa
    23041 aggcacaaaa gtagcatcga ttccattgta gagcgggctt tgtcctaatg cgaattcggg
    23101 atccagacct ttgtatccgg tcagtgtggc caggttatcg cccgaagcaa agattgtgca
    23161 ggaacgaatg atatccgact taaacggcat ttgataagac agggtcaccg atttcagcct
    23221 caggtacgaa ccgtcttcta tccagcggtc agaaaaacgg gcattaccca tcggatcgcc
    23281 atacgctgcg cggggaatat cggtcatgtc accttcagtt ttccagcggt ttaggactgc
    23341 ctgtatctga ttatcaaagt ttttcatgct ttccaattgg gctctggtgt aattataaat
    23401 atcattaccc aatgagtatg tacagaacac attcaagctc cagtttttat atttcagatc
    23461 ggtaaatact gaaccgaaca aatccgggtt cggatcaccg atgattactc tatcgttttc
    23521 atctatcagt ttgttggtgt tgtcggtgtt tacaaaccgc atgtcaccgg cactgaaggg
    23581 ataagtaagt ccgccctttt ggatcatcag gccttcattt gctgcttctg cttgcgtagc
    23641 gtaaacacca tctgttttgt atccgtaaaa tactccgaga ggctgtccaa cgcgggttaa
    23701 tacgtatccg ttacaaattt cggtgatgta ttcttcattc ttcaaatcag tgactttatt
    23761 cttataaacg gaagccacga aacctgcgtc ccatttaagc gttccgttca ggatgcggga
    23821 tttcagcgag aaatcaatac ctgtattgct catttttccg gcgttagtca gatagaaggg
    23881 gaaaccggtt tcgcgcggag ttggtatccg caaaatcatg tccgagatat tattcttata
    23941 gacgtcaata ctgaaattga tacgttcatt gaggaacgag gcatccacac ctgcattcat
    24001 tttagctgtt ttctccggtc taagattcag gtcaacgaga ttacctctca ccagaccaaa
    24061 actacccagc aaactttgcg gaatgtaata ctgaacaccg ttgtagttac ctatttcatc
    24121 gttacctgac aatccgtagc caattcgcag tttgagcagg tcaaacaggc tggcatcctt
    24181 catgaattct tcgccactga tgagccaggc tgctgaagct gacggatata aacaaaacgg
    24241 atttgcctct ctgccatacc gggaggaagc atctgccgac atggtataat tcaggaaata
    24301 cttatcacgg aatccgtaat caaccgttgc aaacaaattg agccagttcc agttgctgat
    24361 ctgccctccg ataacgcgat ataaaggatc accgtaagat atggatttga aactgtctga
    24421 gccggtattt gccgtttttc cccaatcgtc ctgagcctgg tttgtctgat ttctcatacc
    24481 gcctcttatg tcaagtttat gttcgagatt aaagagattg gtataactca atgctgcttc
    24541 ggtatatatg gaagaaagcc tgtcaacccg atgttgcgac acattatcga ccggaccgat
    24601 ggaaagcgta tcgaaatata caccccggtt cggaaaataa agtttttctc tttctttgtt
    24661 gaaattaaca cccaggatag cattaaattt caagtgttta ttaaaatcgt attttgcttc
    24721 gaaattaccg acaaaacgga acagcgaatt atcccggctg ctggtattaa ccagtgaata
    24781 agggttggaa ttattgaaaa catcagcatc ttcaatcacc ggcgaaacag ctccctcggg
    24841 gttattgaca tgtgaagcca taaaaggcgc tttgaccagt gcagcataca aggggtttct
    24901 gttggtcgat ggaccttcgt caatcagttt tctgtttgaa aaaagaaaac tcatgttggc
    24961 aaacaatttc agttttttgc tcaggttaac ttcggcattg aaccgggtat taaaccgttc
    25021 gaaactggta tttttcacaa tgccatcctg gctcatgtac ccgactgcaa gcgcatacac
    25081 tgctacttcg tcgcccccga atacgttcat gtaatagtcc tgattgtagc tgaggttata
    25141 tatttccttc tgccagtttg tgttgttgtt gtatctgtag taatccacat tgccttcgta
    25201 tccccatttc tggtttaccg gtttttccat attcatgtaa ggcaaattca taatctgaga
    25261 cccggacatt cctgtagatt gcaacatatc cgatatcaac agtttatgat ctgtagcatt
    25321 tagtacggga atttccagcg gttcgagatt aacaccggta tggagatgaa aattaatttt
    25381 cgtacccatt tctttggctt tcagtgtgcg tatgagaatc acaccatttg ccccttttac
    25441 tccgtattgc gatgttccgt ctttcagtac ggtgatgttt tcaatatcct taatgtcgat
    25501 ggaagcaagc gggttcgaga aataatttcc aagtaaagag gtagaatata ctgtattttc
    25561 gtaaggcatc ccgtcaacaa taatcaaagg ctgaataccg gcattcatcg aattcaatcc
    25621 tctgatatag atattggagc ctgccgcgag agcacctgaa tgatgaatta gatttaaccc
    25681 tgacacttta ccctgcaagg cttcgtcgct ggtaatggca gtgctcagaa tgttattttc
    25741 gttgattgcc gaccaggtat gtgtcaactc aacgggagat gaaattccct gtggtgttgt
    25801 tacattttcg aacacactcc ggaaaccttc tgtatataac tcaacggcaa tatcttttct
    25861 gccttttacg gaaatcacct tcgacacata tcccggccct gacactgtca gattgacatt
    25921 gccggagggc agattcatgg agaaatgtcc ctcattattt gtcagcgtag tttccatatc
    25981 cttgaaagcg attctaatcc ccgacagcgg tttctgcgtt tcggcacttt ttacaatacc
    26041 tgaaactgaa tatgtgaaat tatcctttgc ctgagcgttt acagttgaat aattaaccac
    26101 acataagagt aaaaacggga tgatatattt acctattctc ataatatata ttgtttacaa
    26161 atcacttatt ttctatttat tcattcgggt caacaaccgg aaccagtttg atataatcca
    26221 gaaataccat tccgcagttt gttgctttgt attccgccgt attagccaca aatacagtag
    26281 cttcgccgaa agtcggacaa ataattgttt ccttatcgcc aaaggtatcc gtgcccgtaa
    26341 agtagctgaa ataatcatcc tcgccggtcg gagtcgcgtt tttgcgcaat aatccgaaag
    26401 gagcctgagt tgcatcctcg acccgaagat atctgtacag ttgtttttct tcctgtacgc
    26461 ctgctttgaa acgggtggaa gcaagtacgg ttgtctcgct gaaattgtcg gcaatggatc
    26521 catcagatgc tttacggctt actctcggtt tatcgggaaa actgatcagc atcttctggc
    26581 taaacataca tgtaacagat gtgttgacat ttactgtttc ttttccggtt ttgatgctta
    26641 atgcaaccga ttgattgata tgatcggagt aatcatcata accagaccag tataccttgt
    26701 aagccactga gtttattacg ggtctgtact caatgtagca gttgttggtt ctaccggcat
    26761 tactagatga gttattcgga gcaaaagtgc gtgcaatggc caccgaaatg gtagtatcgg
    26821 gattagaata aatatattgc gtattatagg acgttgggct gttgagtgtc atatccttac
    26881 caccactcag tgtaggcctg taacgcatca tccacgcatt catgttgtcg gcatagaaac
    26941 tgaagtaatt ttcgccctct attttgatgg ttttcacctt gttttcatat attttcacat
    27001 cggcatcgga caacttatag atacgtccgt tggaggcggc atacgtttcc agaatctttg
    27061 aagcgctgat atccatcata acaccgtcaa cagaaatata gcgaccatcg gcagtaatta
    27121 caaccggcat caggatacaa tctttaatca gttcggcgcg agtaatcgaa tcctgcaacg
    27181 ttttattctc tttgataaaa tatttggcat atttggtttc aatgcgttga attacttcat
    27241 ttggcagcaa agcgaaagta tagttttttg tttcatcgtc gataggatat gcgctcagga
    27301 acggatttct attaatccat acggtgtcgt agagtggttt tccgctcttt ggatcaaccc
    27361 ctgtctgtac acttttcgtc atgtccatga tcaaccggtc ctggtcttta ataaattttg
    27421 cctgcaggtt gccggtctgg gtttgaagat attcccagat atttttctga ccaggaataa
    27481 caccgttcat taaatgtaaa ataccgttgc cggcagataa attgaacttt ccggtttctt
    27541 ttatgagact gatgccgttt atctgcaaac catttacaaa ataattcttg gaattgatca
    27601 tttcaatcct gtcggatgaa aaatggtctt ccacaatggt gtaattggag tatgccaggt
    27661 ggttttttac caaatttttc aaaccttcca catcgttcat atcgacagaa gccagcgcgt
    27721 cattaacagg ggcaaagacg gtaagcattt tatctcccga gagcagcata tcatatccgg
    27781 ttttctccaa tatcgaggca aaaacggatg tttgaggatt ttctgcaatg gcctgaaaaa
    27841 gtgttttgcc tcttaaatcc tttgcagcta tatgttcttc ccatttcgat tcacaggctg
    27901 taaatatgaa cagtatgaag gcagctgcca tgtattttac taatgatttc atgcgtaaaa
    27961 tgttttattt tttataattg tagcatctct tatcccggct gaccggtaaa ctcatcccga
    28021 aaaacgggag ttgtttttat gcaaatattt tttttattcc atcaccggag agtcagcacc
    28081 gccaaaccct ccgggttata acatcccttt attctgttat tccgacggtg gcactaccga
    28141 acagtcggag tagggataaa tctcacattt aggggtttcc tgataaatca gtttgccggt
    28201 attatcctgt tttggccaaa cctgatcttc gtcaatcggg ataaacagga tctgatccca
    28261 ccaggtggta aactgagatg aattcagcgg ctccaggcgt aatgtatgct gtccggtggt
    28321 gaatacctga atgataccca ttgcctgaca attgttagag ttactccaga aaccctgcga
    28381 attgatcatc tgataacgat tcccgtcgag cagtgctttc tggaagaatt cattgttgta
    28441 atccgttaat ccgtaactac ccggggtttt attatatccg gtagaaataa ctcccagcaa
    28501 ctgatcttcc ttaccatcct gtttgaaaat agtccggatt tgctgaactg tagtaagacc
    28561 tctgtatgac aaccatactt tatatgttcc gggtacaagc accggagttt taaattcaat
    28621 ccatttcatg gtattggtcg acaacctgaa atagaaataa tcgccatata cgtagttatt
    28681 gtccttggta atggtcgaag gatatccacc acaataatac tgcaatttgt cggtagcaaa
    28741 ggtttttccc caggttatac cggcgcagtc ggtattgtca atgtatatac ttgctccggc
    28801 ttttctgaag tttttcattg ccatcagttc aggctgctcg gtgaaatccc agtacaccct
    28861 gaatgcgctt cgcttcacaa tctgaatatt cccgttaatg tcatgaacta caccatcgga
    28921 acaggacata tcggtatatt cactggttct gtccaccagg attcccggct cgagcacacc
    28981 acctataagg aattcgttga ggagtaccct gttcacatcc tttttcagca caataacttc
    29041 tccgggcact aatgtattga gtgccgattt gttcatcaga tccactacat aggtaaatcc
    29101 ggctgtcata tgataagcca cataattata cagtaacagt gcatcgtctt taatatccgg
    29161 tgatttcttc cgtagttcag tcatcagttc ttccatattg ccaattttgg cagacttaaa
    29221 tccctcgttg tcctgaatga agcaggtaaa tacaatatcc ggatttgttt tctgagcttc
    29281 atctattgtt ttctgaatgc cggaggcatt atagacttct ttccacaatg aatatgtgtc
    29341 cggaagacca ttaattacat ccttaattga tttccgggac tggttcaaaa cactatttac
    29401 cacatgaaca tatccgttgg ctgctttaag gtctttttgc agaattttac cctgacgatt
    29461 gacctctatc gaaactcccg tggcatctga aatagtttta gttgtaatat agcggcggct
    29521 gaagttggtg gccggtaatc ttccatccac aaaatcaaaa gaacgaatag tatcggcaat
    29581 caggtgatat ttaacaatct cggctgcctg aacttcagtg agtgaattta atgtcagact
    29641 gttttcggtc agatatttat caacagcttc atttgtaggt gcaaacaggg tatatgttcc
    29701 ataagcatgc acagtgcctc tcagacccga tatatcaata attttcagga atgaggataa
    29761 ggtatcaccc ttgctttcca aaatctcatc tatcatcttc tgatcggata gctgatagat
    29821 tttcccctcc atgtcttttt gacaggaatt taatccaaaa gccaaaagta taaacaatac
    29881 cggcaaagca gcgataatgt tttgtatctg atttttcttc aactgtttca tgtgtaatga
    29941 tttttttact ttcaggttac taacgtgttc catagaatgg attttgtgtt aatgctttgt
    30001 ttgttcttaa ctcactttct gaaattggca gataatgact gttataatct ccaccccatt
    30061 tgctctgcaa actcagcgct ttatcgggac ttgcagcata tgctgccagt tcaatcaggt
    30121 aggaaatacc tgcataatta ttccgtttgg cattcctgag cacatcgaac catcgtttgc
    30181 cttcgtgtgc aaattcacgt gcacgttctt ttaatataaa tttttccaga tcattcgggt
    30241 taatattatt ctcatcctga atttcgttcg atgtttcggg tgcgcaggct ctgctacgga
    30301 tggttttaac taattcgagc gattcaacca gttgagctgt atttccgctg gttttcccca
    30361 tctgacataa tgcttcggct ttcatcagaa taatatcagc caggcgatag aaaatccagt
    30421 tactaaacga catgccgtcg gtacgatagg tataagatgt acgcgataat ccgatccatt
    30481 tccatacgta accctgtttg taatcaactc cttcaccccg gatatcatac catccacggt
    30541 caaaagcaga gggcggaaac aactgatcgg aggacatcat ttccatattg gcaaccagcg
    30601 aaccatagac cggattgaat aaatcgtaaa atggattttc atgatcagtt ccgaactgca
    30661 attcaagaat actttctaca gaattaccat tcgcgtacat attcagaaac atattgtcaa
    30721 cgtcaccttc cgaaggataa taggcagtaa tcgtatcagg tggagaaatg gatattactt
    30781 caacaggtgt attctgaaca ggaacaagag taaattgtcc tgaattgata atttgattac
    30841 actgactgac acaattttca taatcatctt tccacagata aatatcggca agaagagcct
    30901 tcatgctcca cagggtaaat ctacccttgt tctcttcttt ttttgatccg taggtatagg
    30961 gtattccgta atttgttccc aattcggctc tgtcaatgct tttcaggtca gaaatgagcg
    31021 agtcgattat ctggctgcca tcggtcttcg gtacgctgta attctgcgca tcgctcagcg
    31081 atgctgtggt gacataaggt acatccctga aagtgcgaac cagataaaaa tacatcaacg
    31141 cccttataca tttggcttcc gcttcgtatt cagacaacaa ttgatccgag aaggaaccgt
    31201 ccagtttctg agtttcttta gcaaaactca gtactgtatt acagttgtta atgactgtat
    31261 atatatcacc ccaggaacaa tagctcatgt ttggcgaaat gtcaccattc tggaactggc
    31321 tcattgctga gaggttattt gcacttacac gtggcttgat caggtctccc cggagttccc
    31381 cccagaataa aaagtcctgc atggcacccg tagtcatcat acttgcataa cagcccatca
    31441 aagcatcttt tacctcttct ttgtttttcc agaatttggt tacgataacg ccatcaacag
    31501 gttcgagatc gagataaccg gaacatgccg aaaaaccggt ggaaagcagg atggtaattg
    31561 ctataattat tttgttcttc atatcggtgt tacatttatc tgttcattaa aatccaattt
    31621 ttaatcccat ggtaaactgg tagggtctgg gtgtgcgtga atagtcacga ccgttactgc
    31681 tcagacttac ttccggatct tgtccggtat agttagtcca ggtatataaa ttttgcatag
    31741 tcagccacaa gttgagatcc tgaagaccaa tgcccttaat aagtttttta ggcatgttgt
    31801 attttgcagt tactgttttc catctcagga aggaaccatc ttcaacaaaa cgatcggatc
    31861 ctaaccagtt gtaaccggct gcatacaaag cgcggggtaa caagtcggca ggagcctgat
    31921 cttcaaatcc cggttcgaat ggagttctga agcgtttaag aacagccttg ctctgattat
    31981 caaagctata cattttctcc atgttcatcc gggtcatatt aatgacatcg tttccgtatc
    32041 tgaagtggaa ccagcctgtt aaactaaaat tcttaaatct gatactggga ccaaaacctc
    32101 cggtaaacaa tggattgtag tcgcctaaat acacaacgtc ctgcagatta atatttccat
    32161 ccttgtttac gtcaacataa cgggcatcgc ccggctggaa aatataacca atagagggat
    32221 agttgaaccg catataaacc ggttccatct ctccggtctg acccatggtg taaataggat
    32281 tacctttctt atcaatagca attgtctgat tctgattcag atatactcca ttgtacatat
    32341 agccataaaa tgagccatag ggctggttga gtacattccg gatgaggtat gaaccattag
    32401 cctgccaggt tccggattct gttcctgcat attccgataa ttttctcaga taattctgag
    32461 agcgtgaaac attgaaactc aaatccactt tccagctttt attccggtag ggtgtactct
    32521 gaatgttgaa ttcccatcct ctgttttcca cagtgttcag gttcatagtt gtattataaa
    32581 atccggaggt tgacggaagc gccgatgtgc cgataccatc cacagtggtt ttgatataat
    32641 attcaaattc catgttgaca cgattgtcaa atccttcgaa attcaaaccg atatttttct
    32701 gagtggatac ttcccaatgc aggttatcca gagaaatgga tgcgggatat gtggctgtgt
    32761 tacccagata attccagtcg taattgttgt acttgctgta gtacatataa ttactgtttg
    32821 gcggattacc attcaaaccc catgaagctc ttaatgataa ttcattgatc cattttattt
    32881 ctttcataaa cgattcgcct gaaattctgt atcgtccgga tatcgccggg aaaattccgt
    32941 aacggtagtt tttcccgaat ttcgaatcac cgtcggtacg gatactgcca taaataatat
    33001 agcggtccat aaatgtataa ttcagattgg catacagtga tagctgacgc gactgcgaga
    33061 aaccggctcc gattgaacca ctaggataca ccctggatgc aatggaagga tcctgtaaca
    33121 tagtagaagg caggttgctg ctcgactcgg aatatgagct accccggctg tcataagtat
    33181 taagtcccag cagaccaatc agtctgtgtt tgtttttatc cttgaatgtc gggatccatc
    33241 ggagtttgct gtaagtttgt acagtaaacg agtcgtcatc gcggtccgaa gcccagttgg
    33301 tagaactgtt gttccattct aggccactgg ctgtctgagg taaatacaac tgagttttat
    33361 tgttgtttat cgacaaaccg atatcaaatg aaaactgcaa ctgaggattg aagtcgtaaa
    33421 tcaacgaaaa tttaggcagc gcgcttacac ttcccttgtc cacccgactg tcattggcca
    33481 tggccacagg attgtacacg ttggggtatt taccctgaat gttggagtag ggagtgaaat
    33541 attgcgaagt cagttcgcca tatacgttgt agtaataaat actttgattt ggcatcttaa
    33601 tataggcctt actccacaag tcgccactca tattattgga agcttcggga ataaagttgg
    33661 actgcgtcgt ggaatttgag taagcgatat cggcaacgaa acgcaatttg tcagacacgg
    33721 tgtagttaag gtttgcacgg gtattcagtc gctgaaatcc ggtgccgata gatgttccgg
    33781 tttcgtccca gtatccggct gagaaacggt atcgaacctt tttactacca cctgataaag
    33841 agatattgta gtcctgggca taaccgtttt tggtaagtgc tccaacccag tcggtgttat
    33901 ttgaatagtt gtaataatac tcgggattgt tgggatcata ggcaagttca gggtaagttg
    33961 ctgtattgag cacttttcct gcattaaaca tagcttcatt gaccagtgta acatactcat
    34021 aacccgataa ggtgggaaga gtcgggcggg gagcattcag tgtgaatgtg gtctgaacat
    34081 caattttggg aggactaatg gaaccacgtt tggtggtaat catcagtact ccgttagctc
    34141 ctctcgaacc gtaaatggct gaagcagcag catctttcaa cactacaata tcttgaatat
    34201 cagagggagg aatatttaac atctgcgaaa attcttcttc ggtagctgtt gcaaagtcga
    34261 aactggaaga agaaccgatc tttgtttcaa gcggtatgcc atccaccaca attaacggtt
    34321 cgttatttcc attcagcgaa gtaagaccac gcacccgaat agccataccg cctccggggt
    34381 taccggtacc acctacgata tcgacaccgg ccatacgtcc ctgcaatgca tcatcgatgg
    34441 aagaagtgtg cactccttcg agttcatcca tcgagatttt tgacaccgcc atcgaaagat
    34501 cacgctcatc gatattgagc cctccgagac tattttgtac cttggcagta attaccactt
    34561 catccagggt tttgttggaa tcttccagta caaccgtgat tacacgttgt gtgcggggaa
    34621 cgattatttt ttcctgaaat cccacatatc tgaataccag gctattattc gtattcgtca
    34681 tgttgatggt gaagttaccg tcgatatcgg taatagcact tgccagtgta cgattatctt
    34741 tatctctttc aattaccgtt gcaccaatta aagcttcgcc atttacatca cgtatctttc
    34801 cgctaataat tgttttagtt tgtgcctgcg acacaagggg tagtgataac agtaatccca
    34861 gcacaaaaca atatatttta attgagtttt tcatgcgttc gttatttatt tttattgttg
    34921 tcgcatgaaa cccgcattga acaaggtctt ttatttttaa catctgtata aatgctcgtt
    34981 tcatgtactt atccttttaa tgtcagaaat ttaagcaata aattttttgt ggtaatacgc
    35041 ttattctttt tgagttgtca gcgattttta aggattaatg attttgaatt tgaagtaatt
    35101 ggtgaattca tgatgaatag ctcttcctgc aatgcggtta cttctgtaac cgttatatcc
    35161 ggttttggtt gtgcctgtca taacacgcgg tgccggagtg gtcattccaa aagaaccgct
    35221 tattgcgagc tttccattga gatatacatt atctggtgtg aatgaaagag tacccgatgc
    35281 atctttgctt acaattacat acgtacgctg attgataaat ctcaacgctt cgttattgta
    35341 ccggttgcag gtggatacaa tattcattgt gggcgagttt acattatagg ggtaaagaaa
    35401 aggctgttta tcgtcaggga atgcaaatcc ttcgagaaaa tgagctttac agaaattgac
    35461 agcgatatct attcttgact gagccagcag tccgtttttc agactgtcca gacttggcag
    35521 atcgccattg gctcttgcgc gggtaatggc gttattattg ggcatcatta tcgtataaaa
    35581 attattttcg ctgatgccat tcagttcatc tgtggttgtg ctttttagtg catattgtat
    35641 ataatccaca aactgggtta cattgatatt ttcacgggcg gtttgctgca ggtaatacca
    35701 caatgtattt gaattccagc cttccttggt cgatgggcta gtttcccgat ttgagtattg
    35761 caacattctg tcaaccgtat aaaccgttcc gttgtcaaag gtttcgcctt tcgttatatt
    35821 tacgaatgta ttatcttcga tattaccgct tgcctgtagc tggttatttt tgtagcgaac
    35881 aacatcgcca ctggtagtgt tacggaatcc ccatccgcca taagcggtaa ttccgtcatc
    35941 gaaatttact tcagagttta ctccggcatc gacccaccct tcgaacagat gcagctgcat
    36001 aagtcttctg agacggtcag taacatttgt tcctacaagt aaagcattgc taaagtttgt
    36061 ggtttctgaa ttatatgtta atccatcatt ccgaagcagt tcgttggaca acaacaaaac
    36121 ggtatatctt gtattaggat atccggtaat ggcacttttc atcaaactct cacgaagcga
    36181 gttctgatag aattttatat aggcagtatt cagtaaagaa tatgccggat tgaggaagat
    36241 ctcggtataa accgtttcga agagtttact ctttataaca tggtcgatgg tgtacacgaa
    36301 accgttgctg gttaatttct tgttgataac gccaaactca tcgaatgtct taccttttgc
    36361 tccttcacca ttgacatatt cacctgtcga tacctgagag gatttcagtt gtgaaggcca
    36421 gatcattgta ttggccatgt gggtgttaat cagggtggta atggcctcaa caggtaactc
    36481 gctgagcgat ttataatact taagcaagcg ggtattgata tagtttgtca aaacttcgtt
    36541 tttcggaacg aacaacgtat atccgtcgga aagtgtggta acggcattat cgttgtaaat
    36601 ggcttcaagg gcgggtgaga atgaaagagg ctgtgttccg gaggtattgt aactttttac
    36661 aaatacttta tcaattgcat cgtttggttt taatattttg tatttttcgg tcagcgtgat
    36721 gtcttcagtg taatttttat aaacataagc gcccgataag tctttgaaat caagcagcga
    36781 tttgaaagcc gaatattcat ttttctgcag gagtttatcc atattttcca tgggtaaatt
    36841 cacaacagat acttcatgtg caatgccatt ccttgctttc atgtttggct tcacgatctg
    36901 gccgttcatc acgtttccga tcataccggt ttctgtactt tgtccgttca catattccgt
    36961 attcgggaaa aaaacattat aatcctgggc agtcagattg ctttttgcaa agaaagactg
    37021 catgaacacc ggataatatt tataattgta tgagctctga ctgtaggtcg atgagtttgt
    37081 gttttgatcg atcacccagc tgttattgta atcgggatct ttgtagaggg tggcataata
    37141 ataggtctgt ttttttagtc cgtcgcccac atcgtatctg tcgcccacac ttccgcggaa
    37201 cagatcaccc aggttttcgg acagccagta agactgcagg atggaatatg ctacaagctt
    37261 attcacttct tcctgtggta tatcattcac acttgcatag ccgtttgcac tcaaatacgc
    37321 ggcaaaagcc tcatcattgg gagccatcaa ggtgaaatag cctccttctt tcaactgatt
    37381 ggcatagagt gttctgtcta tgcatgccaa atagctttta aaatgtcctt cattttgaag
    37441 tacctgatac agtggctttt ccagccagtc cggttctgca aaataatctt ctccctcttt
    37501 attacagttt gcaaaaagga ataaacagag caaaagacta aaatagattt taattttatt
    37561 gaccatatct taactatttt attacaatta cgtttagtga atatatatag atagcacaga
    37621 atttttcctg aattctatca caagtaaaaa aaccagtatg tcaaataact atgtgttatt
    37681 ttatgatatc aaacgtatta gctgctaatg agcctttccg gatttaccgg aataattatg
    37741 aaatgtgata taaatatgtc acaaaaataa aaaatatggg tttctcaggt atgaaagaaa
    37801 tgattttttt gttatggttt ttgacataaa gacattatca atgtttgatt taagttatag
    37861 ggacgtttta atgtgcttat ttctgccatg tttggaagat gctaaaaaca gttgcacaac
    37921 atcattttta ggtagattta attacaatat ctatcaggaa ttctaaccgt tttttattcg
    37981 aaattgttct gtcgtattct gccaaatgca ctctcctgct ggcaactttt ttaggtaagc
    38041 ttcctgctaa aagttaagca ggaaaataga aaagtaaaca agtggacaag tttacccagc
    38101 ctgcataaga ccggattttt ccttgtccac ttgtttacct ttttactcgt ttacttaata
    38161 actataatct tttctgtacc tttcaatccg ggtgccgaaa ctttaatttt tgcctgacct
    38221 gcggtttttt ccgaacgtag tatagccatt gcaaatccgt tgtataacag gcgtttgtta
    38281 ccgtcaaaaa gttcatcact ggtgtggtcg ccgttatcta cggctatcag ttttgcatta
    38341 cccataacat cgaaggtaac ttcacctgtg gtagccggaa ccgtgcgtcc tttgctatcc
    38401 actgcatata ctttcacgta ttgcagatcc ataccatttg ctttaaaatt attgttttcc
    38461 aattctattt tcagcgcaac cgcttttccg gtggtttcaa gctggtgacg agccacttcc
    38521 tttccatttt tccgtgcaat ggcggtaatt tttccggctt cataaggtac attctgccac
    38581 aaaatcatat tccgtttttt aatatcatct gtgatattct tttttattcc gatggatttt
    38641 ccattgacca gcaattcaac ttcatcgcca ttggtaaacg taaagatatt gtagcttttc
    38701 cccttttctc tgttccaatg atctgataca ttgagcgtac cgatggtttg ttcgttccat
    38761 tccatacttt ccgatgcttt atccaccacc ccgatatgca caacgggttc atccgaaaag
    38821 atgcttttta tcaggtaagc ctgtggaaac ggaagtaatg catgattaaa atacgaataa
    38881 ttccaaccct ttcgtggcca gccatgcgac tcgccccagt attcaatagc tccccagtag
    38941 gcaagtccca ccattttttc gcggtccatt ccgaaaaaag gctgtgccag ttcattcgtg
    39001 gtagcttcac tttggtatat aatcatatcc ggagcatgtt ttaagtaatc ctgataatta
    39061 aaccagcgat agttgaaact cgaaatttcg gtaaccgttg ccagttcagg aggatagatt
    39121 ttcgtgttga agtcggcatc cgttttggtt agcgcaccgg cacgagcagg aaacatagct
    39181 actgtggttt tacgggtggg atcataacgc tttgccagca cgtcgagtat tttgtaagta
    39241 gtaacgcccc agtcgcctgt aggaaaaccg gctaaatctt ctctcatttg taactcatta
    39301 ccgaaactcc acataatgac cgacgggtga ttgcgatcgc gttttatcca ttcagtcagg
    39361 tgcttgtacc agacgtcagt ccagggttcg cgtcctgccc agtaatcttt gttgctccat
    39421 ttgtcgtaaa gttcgtccac aaccaaaatt cctttttcgt cggcaatgcg cataaatgct
    39481 tcggaatatg gattgtgcga ggtacgaacg tggttaaatc caaacgattt caaaacatca
    39541 aattgtctgg cgattgctgt ttcaaaagca gcagcgccta ccgctccaag gtcatgatga
    39601 ttggcaattc ctttgagaaa tactttttta ccgttgagtt taaagccaaa ttctttagaa
    39661 aattccagcg tgcggatgcc gaatttttct gatgttttat caataatatt cccgtctaaa
    39721 gtcagtgtga tttcagccgt gtaaagatta ggtgtttcgc acgaccagag ttgtggatcg
    39781 ggcacattga gttcgggcag aagcacttca tcggtgcttt ttttcgattt ctgaggagcg
    39841 aaaattcttg tttcaccaac ctgttttcca tcgggcgaaa atattctggc aagtatttcc
    39901 acatttaaag tttttccgcg tattccttcc acttccacct gaacattgac cgacgcgttt
    39961 tcagtagtta ttttcggagt ggtcacaaat acgccatgac gggcaattga aacactgtct
    40021 tttacaaaaa cgtgtacatc tctgaaaatt ccgcctccgg tgtaccacct tgaatttcct
    40081 tcacggccgg tgcttgcccg cactgccatc acattatcac tgtcgtatct gagtttatcg
    40141 gtcatgtctg tttcaaagcc gagataaccg taatctgttc cgcctacttt ttcgccgttc
    40201 agccacacat cgcccgtaag cataattcct tcaaagtcaa cataaacctt ttttcctttc
    40261 cattcgggat ttgccttgaa ggttttgcgg taccaggcgg tttccattct tttgaaacct
    40321 ctcgcaccgt ttgcactttt atcccagggc tgatttatct gaaaatcgtg tggtaaatcg
    40381 agcgttttcc attggttatc atcgaattgt actttttcag catcctttaa atcgcccatt
    40441 gaaaacttcc atccgaaatt aaagagttcg gacatacgaa tatcttttgt ttcttgtgct
    40501 gataaagata atgttatcag acacaagaaa agtgatacga aaaatagctt gtttttcatt
    40561 gtattcatag ttgtttagtc agctttcaga acaattattc cgtgttttgg aatagtgaat
    40621 gatcttgact gtccgaattt gcccagattt ttgtgcagcc acaaatcgcg taatttgtta
    40681 tttttggtca atcccgcacg ttctggcgta acttcgatgg ttttttcttt ttcgctgcgg
    40741 ttcatcaggg ctatcgcttt tgatttgcca tttttaccga gtggtttcag ccatatctcc
    40801 acatcatctt ctttagctac tctactagcc tgcttacatc ctttgtcctg atttaatgca
    40861 attatttctg tgtttgtcag gatttcgata gtttcccggg acattgttct cagatcgttt
    40921 ccggcgagca atggcgagtt catcatacac cacatcgaaa aatgcgtttt gtcttcatcg
    40981 tagctcattc ctcgtcccac ctgaagcata tccatgtcgt tgtaatgacc aggagagcag
    41041 tatttatgga ggtctgcatt caaatcgatg atatgtaaaa tagatgaaaa tttatcacta
    41101 atatcacctg agatcctcca ggagtctgcc tttttgatag cccattctcc cggaaactgc
    41161 caccggcaga tgttgaatac tatattggga ttaatggctt tgaccacttt gataattttc
    41221 aggtattcgg tttcctcatc aagtttctgt ttttcgccac cacaccagtc cactttcaga
    41281 aaatcgaatt tccagtcgtt gaaaaacagg ctgcaatcgt tctcaatatg tccgtaaatt
    41341 cctgctccaa caccgttttt atcattatcg tacatcgaac cgcaggtgct ttcaccggca
    41401 tcggtataaa ttccggcttt gagtttcctg ctgtgaatat aatccgtcaa tgcacgcata
    41461 cccgaaggaa atttctttgt atcaatctgc agatttccgt ttgcatcgcg accaccaaaa
    41521 tagccgtcgt caatattaat aaaccggtaa ccgactttgt acattcccga acttaccagc
    41581 gcatcagcct gctcccgaat cattttttcg tcgatattgg tacggaagtt attccaactg
    41641 ctccaaccca taatgggtgg tttttgcgaa aaagaatagg aagaaaccag aaacattaat
    41701 aagactggca aaaatgtttt tttcataata ttttatttca atgatttgtt taatctcaca
    41761 ctgtgaggat ttatttaatt tcaactccgt ttactttcac gttattcaat ttcagatttt
    41821 cgataatgct tttatcaaaa tcgctgtttt tattggcttt gatgttcagg ttttcaaaaa
    41881 tgaaatcagt taaaacatat ttttctgatt ttgccacatc aaagtatgtt tcgcattcca
    41941 ggttaatatt tcgcatagta atattgcctg agcgggacgg tggcggagcc tgttcacctt
    42001 tcagatcgaa aaattgtgtc catggacgaa cagaaataaa cttgcctgta ttaccggtaa
    42061 tatcctcaat aagaatgtat tcatatttct gcggggtatc gggacgcatt ttgaggtgga
    42121 gcaacatttg cggatttctc agcgtacaac gtcgtaaaat cacattgcgg tcgtgaatgc
    42181 tctcgctgcc gcaagtgagc gcgctgtgac aaaaaccgta ggtacaatct tcgatgatga
    42241 tgttgtaatt tcctccgttg ttcgggtctt tgtcagaaac gggtcctttt ccccccttga
    42301 gcgcgatggc gtcgtcgtta acttccatat aacaaccttt caccaagaca ttggaacaca
    42361 catcaatatc aatggcatcg gtactgggag attttaccgg tttcacaggc gaaaagaaat
    42421 gcacattcaa tattttcagg ttgttgcatt tgtagtaatg cgatgtccag aagggtgagt
    42481 tcaccatccg gatgcctgaa atctgcacat tattagagtt ggaaatatac accaggcgcg
    42541 gacgcatttc atccttgttg gtgcatttgg gattccatgt acggcgaagc cagaaggctt
    42601 tccagtagcg caatccgttg ccattgatag ttcccttgcc cgaaagtgta aatccatcca
    42661 ccttgtcggc attcaccagt gcaggaaaat aattgcatgt ttcaccttcg atacgcgttt
    42721 ccagcaccgg aaaatcactg atgtcgtcac tgcctttcag cacggctcct tcggccagat
    42781 gaagatgcgt tttgggtttg aagaaaagtg aagcagtcag aaaagttcct ttgggaataa
    42841 ctatgacacc tccacctgca ttagcggctt tgtcaatcac agcttgaatt tgtctggtct
    42901 gaagcagggt gctgtctttt tttacaccgt atttggtaat gacatactga ttaccaaggg
    42961 tcttataatc gactgtttcg gtctttcgga accagtccgg tatcggtgta ccgtccggaa
    43021 agaggtcgga tgcatacgta tggatgctta ataaaactgc gaaaagcagt ataacgttca
    43081 ttttcatgat cttcattttt ctatatgggt ttctagtatt ttttggtatt gaatttttta
    43141 aaccttccat gcacattcag ccgataccgt gatatcggaa ttaaagatct ccggttaacc
    43201 tgttgaagta cttttgctgt ttgttttgct gttcgggagt ttgatatacc cgcacatagt
    43261 caatctcgta tttcatggga aaagcattta tcaccggttc acccccgtgc tgaccaccaa
    43321 ttgccaaatt cagaagcaga tagtgaggct gcctgaaagg attcgcgtta tctccaattt
    43381 gaccgttaaa agtttcggac agagagattt cgtttaacaa ttcatcatcc agatacagct
    43441 ttatggcatt ttcatcccaa tccattctcc agacatggaa tttcctgccc cattttttgt
    43501 ctttcgacgt gaaatgtgag taaggtattg cctttgtgtt ccatacgggt ttatatcttg
    43561 cttccgtgcc ccaggcaaca ttggcaagaa tgtgtggtac tccctctatt ctgtaatatt
    43621 ccattatatc aatttcgccg caggagggcc actccatcga gttgcccagg gtccagattg
    43681 caggccatac cccgctttcg agcggaattc tggctcgtat ttcaaatcga ccgtacagaa
    43741 attctttctt gtcgcgcgta tttattgacg atgaagtata ttctataaat tcacgtcttc
    43801 tgcgccaatt ttcctgacca gctgaatata gaggattctg tattttatcc ctttttgctt
    43861 caataatcag cactccgttt ctgcagtatg cattttcttc ctgataccac tgcaattcgt
    43921 tgttgcgaac aaaacctttt tcgaagctcc agtaagccgg atttggagcg ccatcagctt
    43981 tgaattcatc actccaaacc aacttgtaat cacgggcatt tacaaaaccc ggaaaattac
    44041 aacagaaaaa aactacagaa aacaataatc gtatctgttt catggataat cattaattgt
    44101 ttgccaaata tattataata agcttaaacc acaataactg tccataagaa aacttttaca
    44161 tacaattact tagcagtaag cactgccgac ctgctttcaa tcacctcaat ggttggagtt
    44221 tccaacaggt aatcagcccc cttaaaagta attccggctt gctgctgaaa atatacaggt
    44281 tcatttttaa tcaatggtaa tatactcaat ttcagatcat tgccaaatat cttatcagca
    44341 aaatatccaa ggttgatatc catcggtttc ccgttataaa aattatctgt aagcagtgta
    44401 tcgcctgcat acactctggc cacatcgcct ttatatgtaa ttctcaatgt caggtttcgt
    44461 aagctaagat catactctgg aagtgtaatt ttccattcag cagctttagt aaaatcctca
    44521 tcacgaggca tttcagccac tttggcagtc ccgaaagaaa ccaccctcaa aggcaaatcc
    44581 atatctttta ttttggttaa cccagcttta atccgtctga tttcaggaag tttgatttta
    44641 tatttcgaaa aaattccatc ggaagaaggt tttacttctt taccattata ttccagcgaa
    44701 ttaagtgccg gatatataaa aacttcgcag tcgcttttgt cgcgaactaa ttccaaacga
    44761 tttccatcat aggtcaaatc agcgttactt ataaaaattc gttcttttcc ggcaaatttc
    44821 cctttccata gtttcagtga agttttctgg ttaagcaaaa taatgctgat ttcccggttt
    44881 gaagcggtac gcaatcgtac agcaggtgaa gttcctgttt tcagtccgct gaggtaaatg
    44941 cgatttcctt ccgtccttcg tttccctgcg aaagatttta cagacacttc ataattgtca
    45001 atcgccaatt caaccggaat cccttgtatt tcagaaaaaa cataagttgt acgacctgct
    45061 tcctcgattt tggcaacagg ctgagcagta gcataaacca gtttagcacc gttcagatcc
    45121 atattgaaag gtatgaaaaa tgcagaaccg gagggaacac tcattgtttt ttggggtatt
    45181 tgcaatttct caccgggaaa atcgagggta aattgtatgt cattttttgg acttaatgtt
    45241 tttagtcgct gatagttgtt gacaaaaatg tatccgctgt tgtcgtttgt ccgtacagac
    45301 caccggagaa gcgtgtcggt tttgaaatca gtaacggtat tttcaggaaa atacggtttc
    45361 atcagggcta gttcaccacc aaaatctttc aaaaaaaggt gcagtttccg cagcaaataa
    45421 taatgcggat tgatctgtcc gaattcaccc agcggtgcct gaaaatcgta ggatttttcg
    45481 ggtaaatcat tgttattggt atagttgctg gcttgtcttt cattcagggt ggttagttga
    45541 ccatcaggat tattgccgcc atgatacatg taatacccgg gtagattgct gcctgaaccc
    45601 acccgaacca acgccatgga atacacatcc atcggtgcaa tattaatgcg ccggtgatag
    45661 ctggtcatca ttccgccacc cagttcgcaa gtaaaatacg gatatccggt gtcgccggaa
    45721 gcatcatttt ccgattgtgc gggaagttgc tcggtagcta tcacggttga actacggaat
    45781 gaacggaaca gaaaactttt tccgtaatcg cccggcattt ctgccaccga acgatcccag
    45841 aaaccatctg cgtaatcacc gtacaaaggg attatctcac cataaggtac cggagaagtt
    45901 aactttggcc atccggttcg ggtatataaa ggcacatcaa aacccagttc aacagccatt
    45961 tttttcaacg tcataagatg tgcaccatgg ccgcggtatt cattttcaag ttgaatgcca
    46021 ataacaggac ctccatcttt ccatagtaag ccatcggctt gtctgtagat ttcggcatac
    46081 cactttttta cttccgtcag ataatcctta ttctcacttc taagtttcaa tccgctattc
    46141 acaagccatt caggaaaacc tccgtttctt acttcaccgt gtgcccaggg accaattctc
    46201 aacacaacag gcatattcaa ctctttacag atttccagaa acctgcgcag atttcgctga
    46261 ccttcccagt tatacattcc tctcacttct tcatgatgta tccaaaatat ataagtggac
    46321 aggatattta tgccgccagc cttcattttg agcaattcac gcttccattc ggtatctttc
    46381 acccgcgaaa aatgaatttc gcccatcaca ggcaacaccg gtttgccatc cataagcata
    46441 tgactggaat ttatcaaaac agttggtcct gacggacttg cactgcttcc cattttgaaa
    46501 gtgctttcga aagatgtttt ttcggttttg ctgattttca ggaaataacc atcaggcttg
    46561 ctacctgccg aaagcgtcat acagatggtc aggagcaata aaattgcagt tgttttcata
    46621 taaatctgtt tcattttcgg ggatttaatg ttacgaaaag ataatcttta aattcgaagc
    46681 cacgaaatgt aaccgttact tccacttttg ttcttgtacc tgcaggaata tctttggtgg
    46741 tcagatagcg cagatgttca ttgccaccca ccgaccaaca atagttatca taaatgacac
    46801 ccgcatcgag catcacatga tcatcgttca ccaccaactc cttctgagga aaacttttac
    46861 ccagatcgct cccaaaataa aaacctggct gagtgggttg attgtaggct acattttggg
    46921 ttgccacgct tattctatac accggatctt cggtaaagct atgaaagcga tactgtgttt
    46981 cgaagggagt aagataaatt cgcagatttt tactgtcgcg ggtcctgtaa atcacttctt
    47041 ctcgccagtc accgaatata tcggcctgaa ggcacggatt ggattttgtt ccattatttc
    47101 tgacacagtc gttattgttg aatagcagtt ctgcaagtcc cgtttcggga taatacttcg
    47161 aaatacgtgt tccgtcaagc agttcgcgtg acaaatcgcc gtcccaccag catgccatat
    47221 tgatagaaac gccacgtatg gttggattta tcacttcacc ctttatgttt cgtattcctt
    47281 cggaagctat cgaccacatt tccacccctt tatgatttgg atcgatatca gctgccatac
    47341 aacgtcctac gtcgatatca ctttttacct gaaaaataat ttttcccgtt tcggcatccc
    47401 ggaatgagga accatctcgt cggttttcgt gacaatccca aacctgaagt tttcggctca
    47461 aaggatcaaa agcagtcata tgtaaggcat cgccatgacc cataccggtt gagtacagtc
    47521 ctttacccgt attatcaata acgcacgccc cgtaaataat ttcatcacag ccatcaccgt
    47581 ccacatcacc aattctgaga ttatgattac cctgtccggt ataggcttca ttacccggcg
    47641 tgtccgtatc gaaaacccac cggctggtga gtttgccatt gcgccagtcg aacgctgcca
    47701 aaacagaacg cgtgtaatat cctctacaca taaccacact gggatattca ccgtcgagat
    47761 aagccacacc tgccaggaaa cggtcagagc gattggcttc aacatctccc caattactca
    47821 agttgccacg gggtggaata taatcgattg tttgcagggc ttttccggtc aggccctcga
    47881 aaacggtaag atattcagga ccggtcagaa ttcgtccggc ttcattccgg tggtctgcgt
    47941 tcttatctcc aatcacatta ccctgtccat caaccgttcc atcacaggtt tttaccacca
    48001 tttctgcttt tttgtcgcca tccaggtcat acaccataaa ctgtgtataa tgtgctccgg
    48061 ctctgatgtt tttgcccaaa tcaattcgcc agagtttttt accatttaac ttatagcaat
    48121 cgaaatatac attaccagta tagccgtcat gcgcattatc gtgagcattg gaaggatccc
    48181 atttcaatat aatttcatac tctccgtcac catccacatc accaacggag gcatcgtttg
    48241 cagaataaaa atatggttgt cccgtcagac tgatatttcc gggtggcaca tcaagcggaa
    48301 tattgatata atcggatgaa ttttcaggta tttcgaatga gcagatttca ttgatagttt
    48361 tgtcagtggt attggaagct ttgatttcat agatcagttt acccggagct gctttcaaat
    48421 ctttgtaata ggtagtattc gaaacgggca tgttgttgat tttttgccca tttcggtaca
    48481 catcgaatgt tgtgcccgtt tggtcggtcg ataaatagcg ccagctgata ttgacagtgc
    48541 tgtcgttctc acgcacagct accaatccgc ggtttaattt ctcccttttc agttttgaga
    48601 aatcatagcc cggtttcaaa gcattgtaaa tcagatcagc aataacctga tgacccgcat
    48661 cattcggatg gagaaaatcg gcataatatt cgggttttcc tgtaagagga gtatgcaaat
    48721 cgatgatacg caagcctttg tttttagcca tccgcctgat aatggggata atttcatcag
    48781 ctatgacttt aagctgatgc ttccagcgtg tatttggcac aaccggaatc ggaagacaaa
    48841 gataaatggc tggttttgaa ggcagaccga gaaactggtc acacaaccac gataggtcct
    48901 tttcaaaatt tgctgcaaat gttcccgtat tccataccgc gggctgagag tcgttcgtac
    48961 caagagcaat cgtcacaata tccggctgta aatccttcgc ttttgtaaat gcttcgagac
    49021 cttcataagg tttataagta ccccggcagg ctgttgcacc gcttacacca aaatttacaa
    49081 cctcgtaagc atcaccaagc aaatccctca aaacacccac atatcctttg tttactctgt
    49141 cggatgcaca agcgccttcg gtgatgctgt tgcccacgca ggctatcttt atttttctga
    49201 agggagcata tttccggtac aaagacctat cctgtgcaac tactgtagta aaagcacaaa
    49261 ggacaaacaa tgctaatatc cagatttttt tcattctaaa tcagattaat aaaataaaaa
    49321 aaggattttc attccggatt ttatattgcc ggattgatta tacagtccaa aaacgggtat
    49381 tatattgaat taaatgatta tccgatatat cgcctaaaat aattatcttt gatgtaaata
    49441 atcaga
//
LOCUS       DAOPYB010000005        46551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_633_length_46551_cov_8.794972, whole genome shotgun sequence.
ACCESSION   DAOPYB010000005 DAOPYB010000000
VERSION     DAOPYB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 46551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46551
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_633_length_46551_cov_8.794972"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="PKH58_00650"
     CDS             <1..1998
                     /locus_tag="PKH58_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX87563.1"
                     /translation="MQTPSFIVTSDAGTGIGYTGFRIRGTDANRTNVTINGIPFNDAE
                     SHSVYFVDLPDLASSLHSVQVQRGVGTSTNGAPAFGASINLQTEKINKNPYSEIGTSF
                     GSFSTLKNTVKAGTGLINNYAVDVRLSNVNSNGFIDRASVDLKSYYVSAGYYGEKTIL
                     KFLTFGGKEKTYQAWNGVDLDYFPRNYNELGKYTDKNGNIQFYDNQTDNYGQYHYQLH
                     FSHILKPSIYLNAALHYTRGIGYYEEYKTDRKYAEYGLTPAFSGEKLFDKTDLVRQKW
                     LDNHFGGATFSVNFNKENYSLILGGAANKYLGDHYGKVIWVRNANNLNVDHLYYFNKS
                     TKNDFNIYGKFNYQIFDNIMTYIDLQYRNLALDMSGDDDKFNSETGKMRVLSNQKTFS
                     FFNPKFGVNYKINTADNAFISFAIANREPNRNNYTDAGPNEQPTSERLYDTELGFSHQ
                     STDFSFGANIYYMKYKNQLILNGKMSEIGELLTSNIPDSYRTGLELTGGIKITRGLRW
                     DGNLNFSRNKIKKFTEFADEYDADWNWTGIKQNFYANTNISYSPDVVANSIFSYTFHG
                     FETALYTTYVGRQYLDNTSDVKRSIDAYFVNNLRLGYTLKLKKVKSIDFNLQINNLFN
                     TEYETNGYNWYSYYMDGKRINEKRYFPQAGINYLASIAFKF"
     assembly_gap    2065..2164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2165..4068)
                     /locus_tag="PKH58_00655"
     CDS             complement(<2165..4068)
                     /locus_tag="PKH58_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNX87564.1"
                     /translation="MISIEQLTVEFGGSPLFDNVGFLINQKDKIALVGRNGAGKTTLL
                     KLIAGKILPTRGKVISASDITVGYLPQHMIHNEGTTVMQETEKAFEHILKLQEDINRY
                     TAELSERTDYESKEYTVLIDKLSHANEHLQIINSGNFYAEIEKTLLGLGFLRTDFDRQ
                     CAEFSGGWRMRIELAKILLQNPDVLLLDEPTNHLDIESIQWLESFLASTGSAVLLVSH
                     DKAFIDAVTNRTIEISLGRIYDYNVNYSKYVELRKERHEQQVRAYENQQKAIQETEDF
                     IERFRYKATKAVQVQSRIKQLEKIERLEVDLEDTSRLSLKFPPSPRSGSIPLEIEHLS
                     KSYGNHLVLDNIGMIVNRGEKVAFVGKNGEGKSTLVKCMLSEITYSGILKLGHNVKIG
                     YFAQNQASLLDENLTVFQTIDYVATGDIRTKIRDILGAFMFGGPASDKKVKVLSGGER
                     SRLAMIRLLLEPVNLLILDEPTNHLDMASKEVLKEAIKAFDGTAVIVSHDREFLDGLV
                     TKVYEFGNKKVREHLGGIYEFLQKKKIENLNELGLKQSPVTTNETPAEKEVSENKLSY
                     EARKELNKKIRKAEKEVDEAENSVENLENEISDMQHQLENPQKASDPDFIILLQKKQR
                     ELEQKMYEWE"
     gene            complement(4065..5195)
                     /locus_tag="PKH58_00660"
     CDS             complement(4065..5195)
                     /locus_tag="PKH58_00660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNX87565.1"
                     /translation="MNQILPFFKTHPYFVSIISFTIGYLFMPLVIEVARKYNFVVSPN
                     KRTSHNGDIPNIGGINIFVSFLFTVFLFSYNVFSEIQFSILGLFIILIVGFIDDLIDI
                     KASWKLIGELIAAFFLIILADLRISNFHGFLGIYELPLFFSYVISFFVFIVIVNSLNL
                     IDGVDGLASGLGILYSLFFAVYFLLSGHFELSVSSFAMFGSLVVFFYYNVFSNKRKIF
                     MGDSGSLLLGYMINLFVFSFLEMNAKHDVPPQLFIKAAPAVAISLLIVPLFDTVRVMV
                     TRVKKGYSPFRADKNHVHHLLLSLGLKHKDVSFVLMGITIGFIIVGLIFRNIPNGVLV
                     AIDFVFCLILTKYLWILVDRKKMPKTEQLVELKQNKDLTEKP"
     gene            complement(5192..7099)
                     /locus_tag="PKH58_00665"
     CDS             complement(5192..7099)
                     /locus_tag="PKH58_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNX87566.1"
                     /translation="MSLRYLPRWLVLSVDIVVCIIAFVISYLIAVNLSVTHNQVLALP
                     FTSRLSLLLLFQVIFFWFFHTYSGILRYSGYVDAAKLLLAVFSNAVLLVILNLLFISF
                     LSIHLFYYESILFYSVISFFLLFIIRLAVKTIYDYISQNSGISIPIMIYGTKSAGIAI
                     AKMIRTNENVKYKLVGFVDDDKNSVEKIVMGVKVYKKTEENVKKIISKKAKAIIVSPL
                     KLQTINPQIDLDIFLNNKMSVLITPPMSIWDGDKMPSPKQIKSIQIEDLLERPQIKIS
                     TENISSVINNKVILITGAAGSIGSEIARQLTSFKPQLIVLVDQAETPMHNLILKLKEQ
                     YPKQDFSSYLGDIRNLERMKSMMEIYRPDIVFHAAAYKHVPLMEDNPTESIQDNVLGT
                     KNIADLSLKYGVSRFVMVSTDKAVNPTNVMGASKRIAEIYVQSLNKKQEKEDGAVTRF
                     ITTRFGNVLGSNGSVIPHFKQQIEKGGPVTVTHPDIIRYFMTIPEACLLVLEAGTMGN
                     GGEIYIFDMGEPVKILDLAKKMIRLAGYIPDVDIPISFTGLRPGEKLYEELLNKKEIT
                     QKTHHPKIMIATVREYDFDDISQKINELITYSYLGKNFLTVSQMKSIVPEFISNNSQY
                     ERLDVENIIAE"
     gene            complement(7273..8910)
                     /locus_tag="PKH58_00670"
     CDS             complement(7273..8910)
                     /locus_tag="PKH58_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNX87567.1"
                     /translation="MMKRICLITCVLLFSLLNYAQKIQPTKNIILMIADGTSPSVLTA
                     SRWTKIYRNAGNKLNIDPWLCGTVSTFSSNAPIGDSAPTTSCYMTGIAAQASNISIYP
                     VADPANDLIRLNPDSAYQPLVTILEAMQKEQNKAAGLVVTCEFPHATPADCASHFYNR
                     SDYKVLAPQMAYNNLEVMFGGGNDYVTEDMKQHFSNTGTTYIKDDKDALMKFNGERVW
                     ALFGQREIPYDLDRDTMNVPSLAQMTSKALEVLNKNKKGFFLMVEGSKIDYAAHANDP
                     AGIISEYLAFDEAIGKAIDFARKDKNTTVVVLADHGNSGFSIGRNGMSKSYTKMTLED
                     LFGTFSKYKRTTVGLEKILLSTKPDSLKQVFKIYTGIDITEDELNSLKGANNYKVDNY
                     MNAANVQNLAYFITKIMNSHSNFGFTSSGHTGEDVFLAVYHPKNHRPTGNIRNNEVNN
                     YLFKAAGLKTSLKEQANEMFAKHTDVFAGMEYRIENQQDKPIKLIVKYESGVLEIPAF
                     SSVGKFNEKPFDIGSVVVYIDKNNTFYLPKNLRKKFK"
     gene            complement(8925..11525)
                     /locus_tag="PKH58_00675"
     CDS             complement(8925..11525)
                     /locus_tag="PKH58_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNX87568.1"
                     /translation="MKKLYSLFFVVLIISLWSCRNNSHFITDTRYRSEVHKQFEKRKL
                     QLTGRKDELFAVFDKDDLKIQEIEALQFLYAYMSLNDLGDYDGDFFYRQIKTAFDARD
                     FFSWGDKVPEELFRHFVLPPRVNNENLDTARIVFFNELKDRIKNMNMKDAALEVNHWC
                     HEKVTYRPADSRTSSPLATVRTGFGRCGEESTFTVAALRAVGIPARQCYTPRWAHTDD
                     NHAWVEVWADGKWYFMGACEPEAELNKAWFTYPAKRAMMVHTNVFGKYYGPESKTEYP
                     LYTKINVLENYTTTKKLNVTVVDEKNNAVSGAKVKFLIYNYAEYYPVYEQITDIKGNA
                     SVTTGLGDMVVWAAKDDRYGYKKVSVSDDDKVVVQLDKTPGKEYQEEWDIKPPVNQAT
                     FHKDTSAITAINNKRLQFEDSIRSVYLSTFLKPDSARLLARKLGLNPDKVTEYIAKSE
                     GNYSEITAYLTRNASNPLVLDLLSTLSDKDLRDTPSRVLESHLLHSKNTDNLPDDVFV
                     KGVLSPRISNELIREWRPFLQKEFKEIFPSNVTVDLIREWIKKNIKIVEDENYSGCLI
                     SPVGVYKLKQCDINSETVFFVALCRSLNIPTIIDPATSEISQYVKGRWEKVVLEPNKN
                     KLPTGNLTINFHSQDDLVPQYWTYYTISKFSDGYFHSLDFEGDKRVEKFPVQLELEEG
                     YYCLTTGNRYANGNVLARNEYFSVSKSKQVVKELTIRELEVENKTYGVLKTSILPSDY
                     YDPRGMVICFLEPDREPTKHIMNELPDYKTQFDKWSGKFVFVVPTDKTGKNFTPDRYK
                     NLPENSTFILNNGEKIMDEFLKSANQSFRDNYPLIYIVDSKGQIIFYSEGYRIGVGDL
                     ISKTIKTYYK"
     gene            complement(11581..12429)
                     /locus_tag="PKH58_00680"
     CDS             complement(11581..12429)
                     /locus_tag="PKH58_00680"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HNX87569.1"
                     /translation="MKFNQLTPEEENVIVHKATERPFTGEYLHNKESGTYICKRCDAP
                     LYRSSDKFESGCGWPSFDDEIEGAVKRVPDADGRRTEIICASCGAHLGHVFEGEGFTS
                     KNTRHCVNSISMKFVTENKKTTDTAYFASGCFWGTEYHFMKAAGVRATTVGYMGGHTQ
                     NPTYKEVCTGTTGHAETTEVVFSPGETSYEKLIKLFFETHDFTQIGGQGPDIGDQYRS
                     VIFYTSPEQKSTAEKYIKILTEKGYKVATKIQAAGEFWEAEDYHQEYYDKKNGTPYCH
                     IYRKIF"
     gene            complement(12642..13115)
                     /locus_tag="PKH58_00685"
     CDS             complement(12642..13115)
                     /locus_tag="PKH58_00685"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HNX87570.1"
                     /translation="MIQRIQTVYLVLVAVLMTLTAALPVAEYFDAVKNISYQLDMRGF
                     VQLNPDGSFLSAVSTNPTTFVFGIILVVTLMTIFKYKNRKQQFRLCTINFILILIYTI
                     LLAVVIFMGKHKLTGTEISLKIPAVLSLVALIFNYLAMRGIAKDENMVKSMDRLR"
     gene            complement(13132..14157)
                     /locus_tag="PKH58_00690"
     CDS             complement(13132..14157)
                     /locus_tag="PKH58_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX87571.1"
                     /translation="MIKFAVIGQGHIGKRHAEMIRRNGDAELVAVCDVLPKEQLGLAD
                     IQEPFFNDIDSLLDSELDIDVINICTPNGYHADYAVKALKARKHVVLEKPIALNKKDA
                     ERIVFKSLEMSRHVFCVMQNRYSPPSVWLREIMDQRLLGKIFMVKLDCYWNRDDRYYK
                     KGNWHGDEQLDGGTLFTQFSHFIDIMYWLFGDITNIRGNFADFNHKNLTDFEDSGVVT
                     FDFINGGMGCLNYSTSVWDTNLESSITIIGEKGTIKVAGQYMNEVEYCHVKDYVMPEL
                     APSNPPNDYGLYKGSAQNHHYVIENVVEKLTDKGSITTNVLEGLKVVEIIERIYQVRD
                     QQWKKEK"
     gene            complement(14181..14756)
                     /locus_tag="PKH58_00695"
     CDS             complement(14181..14756)
                     /locus_tag="PKH58_00695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX87572.1"
                     /translation="MNYFAHETAVIDENCVIGDGTKIWHFSHVMSECVIGEQCNIGQN
                     VVISPKVVLGRNVKIQNNVSVYTGVICEDDVFLGPSMVFTNVINPRSHVNRKNEYAQT
                     IVRKGASIGANATIVCGNEIGEYALIGAGAVITKEVKPFALVIGNPARQTGWVSRNGQ
                     KLSFDDNGLATCPETGEKYKLNDGFVKLLSE"
     gene            complement(14796..15923)
                     /locus_tag="PKH58_00700"
     CDS             complement(14796..15923)
                     /locus_tag="PKH58_00700"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNX87573.1"
                     /translation="MKPIQMVDLKSQYEKIKTEVDAGIQEVIDSTNFIKGGKVVDFQH
                     NLEKYLNVKHVIPVGNGTDALQIALMALGFQPGDEIITPTFTFIATAEVVALLGLTPV
                     VVDVEPDTFNISIDSVRKAITPRTKAIVPVHLFGQNADMEALIEIAREFDLKIIEDAC
                     QSIGSIYTFSDGRQMQSGCMGDIGCTSFFPSKNLGCYGDGGAIFTNDDFIAGKIRAIA
                     NHGMVVRYHHDLVGVNSRLDSMQAAILDVKLKYLDHYIVARQQAADYYDKAFSGCEKI
                     TIPFRNEKTTHVFHQYTLKLNGVDRTALQKHLSELGIPAMVYYPVPLHMQKAYKNSRY
                     KEGDFPVAEMLCSTVLSLPMHTELDGEQLEYITKSILNFIS"
     gene            complement(15969..16913)
                     /locus_tag="PKH58_00705"
     CDS             complement(15969..16913)
                     /locus_tag="PKH58_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87574.1"
                     /translation="MKVKALLISKDALTFLVFLVLSGMFWFVHSLGRERESIIKIPVN
                     YVGIPEDVKIVSELPKDITIVIRDEGLRLLEYNKKKTIPLTLDLTRVYFQQGKILITA
                     EQLQSRLYSYVLPTTAVLSIEPDSIPIYYHKLASKNLKIVLNSDLRLANQYIYSDSIR
                     IEPSKVKVFGPKHIVDTMKAVYSEPIVLHEIADTLLVKSKLKTVNGVKYSFEEINVGI
                     FTEMFTEKKQQIPVTVINVPDKLNVRIFPVMIDVTYNVGLSNFSKIKSNDIQAVFDYK
                     ELKKNQKRKQKLQVINNSPYISNLRFFPEEVEFFLEEN"
     gene            complement(16968..17306)
                     /gene="yajC"
                     /locus_tag="PKH58_00710"
     CDS             complement(16968..17306)
                     /gene="yajC"
                     /locus_tag="PKH58_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNX87575.1"
                     /translation="MNLLSVLLQAAAGKAQPTGGMGGYSGILMMVLIFVVFYFFMIRP
                     QSKRQKEIKAQREAMKVGDKVVTSGGIYGKIKDIKETTVTVEIAENVRITVDKNSVFA
                     AASDIPNEVK"
     gene            complement(17334..18269)
                     /gene="nusB"
                     /locus_tag="PKH58_00715"
     CDS             complement(17334..18269)
                     /gene="nusB"
                     /locus_tag="PKH58_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNX87576.1"
                     /translation="MINRVLLRIKIIQILYSFYKGDGQSATLAERELFHSIEKTYELY
                     FHLLQFSVEITHYAAIKIDSRKNKLRPTEEDLNPNTRFVDNRFVAQLEKNRCLQDFLY
                     EHKTSWANNQDAIKNIYELVIASDVYKEYMEAETSDYAADKDIWRKIYKKIILQNEEF
                     SDSIEDQSIYWTDDLEIVISFIIKTIKRFDAGNDVNQELLPMFKDDEDAEFASRLIKN
                     TLENETIYRRMIDQHTQNWELDRIAFMDIIIMQAALAEILTFPTIPVSVTLNEYIEIS
                     KSFSTDKSATFINGVLDNIVKELKADNKLIKVVKI"
     gene            18568..18987
                     /locus_tag="PKH58_00720"
     CDS             18568..18987
                     /locus_tag="PKH58_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNX87577.1"
                     /translation="MEGEKRKIEIEKRDSEIIYSKAIKAGKRIYYLDVKKSRNEDLYL
                     AITESKKKVTGYDEEAQVTYEKHKIFLYKEDFDKFIEGLEDVVGFIKKEQGEATPRPE
                     YNPDYKKEEYKEEVETETVLEETVGETKRSFFDKFKF"
     gene            19151..20167
                     /locus_tag="PKH58_00725"
     CDS             19151..20167
                     /locus_tag="PKH58_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HNX87578.1"
                     /translation="MKSFASDNYSGIHPEILNAIVQANNEHAISYGDDPVTEETNRLF
                     EKIFGEVKVLYAFNGTGANVVALKCCALPFQSVICTDTAHIHVDECGAPTQSIGCSLV
                     ALPTSDGKLTTELIEPQLINIGNLHHTQPKVISISQSTELGTVYSTDELKALSSFAHQ
                     NGMYLHVDGARISNAVAALGCTLKEATVDCGIDIMSFGGTKNGLMIGEAVLIFNKDLW
                     KNAPYLHKQSAQLFSKNRFMAAQFKALLETELWRKMALHSNRMAYLLENEVRKIEGVV
                     ITQKVQANSLFVIIPSYAVEPLREKYRFYDWNTATGELRWMCSFDTSEEDIASFAATL
                     RSLI"
     gene            20225..21244
                     /locus_tag="PKH58_00730"
     CDS             20225..21244
                     /locus_tag="PKH58_00730"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNX87579.1"
                     /translation="MISESLEKYISNIPKTELHLHIEGSFEPELMFKIARRNNINIRF
                     KTVEELRQAYQFGNLQDFLDIYYDGANVLIHEQDFYDLTMAYFRKCKENNVVHTEIFA
                     DPQTHLSRGVKMETIINGITRAREDAERELGISSYLIICFLRHLDEDSAIETLEEALN
                     YKNEIIGVGLDSSEVGNHPEKFKRVFARAKEEDFRLVAHAGEEGSSEYIWGALRELNV
                     SRIDHGIRCMNDEGLIDYLAENQIPLTVCPLSNLKLQVVKNLSHHPLKEMLNNGLLAM
                     INSDDPAYFGGYVNENFMQTAEALNLSEEDILQLAKNSFKASFLPEEEKEKWLVELDN
                     FQISN"
     gene            complement(21308..22258)
                     /locus_tag="PKH58_00735"
     CDS             complement(21308..22258)
                     /locus_tag="PKH58_00735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HNX87580.1"
                     /translation="MFSTQVNIPHSPFEITFSDRILTLGSCFAENIGNKLLDAYFLTF
                     INPFGVMYNPMSVCQGIRYLLGEKEFTADDLFQSGSLWNSFAHSSAYSATNPKETLQK
                     INSRLLAARYFLKETNVIMITLGTAWVFELADNGKIVSNCHKQPASSFNRRRLSVDEI
                     VSEFTNVLGLLHRQNPQMQFIFTVSPIRHWKDGAHENTVSKSTLHLAVDILEKQFDCV
                     HYFPAFELMIDEMRDYRFYAADMLHPSDVAVDYIWQRFTDTYFSSQTQNLRKELEQLR
                     SDLNHRPMHPETEEYRLFQLAVEKRKTRLIQNYPFLKERF"
     gene            complement(22382..22885)
                     /locus_tag="PKH58_00740"
     CDS             complement(22382..22885)
                     /locus_tag="PKH58_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87581.1"
                     /translation="MKNVLMIMFALVISASIATAQTTTQTKMAELQKKECCDKAKQEC
                     KNATAEAKHECKNAATQQGCKQAKTNCAKADSLKKHECNQAQAMAKHECKSECKNGKA
                     SCCKDGKAMEKNEKGACCKDAKATCDEGKQMTKQCTADCCKDKSTKVTKSNVKKQTAK
                     KTTLAKK"
     gene            complement(22986..23312)
                     /locus_tag="PKH58_00745"
     CDS             complement(22986..23312)
                     /locus_tag="PKH58_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNX87582.1"
                     /translation="MKTKHLVITFVAIFVLATNLNAQSGKSNSKKTVVFDVSMHCESC
                     QKKIEKNIAYEKGVKDMAVKLEDKTVTIKFDTLKTNESKLISAFNKLGYEAKVVETVP
                     AKSTAK"
     gene            complement(23341..25566)
                     /locus_tag="PKH58_00750"
     CDS             complement(23341..25566)
                     /locus_tag="PKH58_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX87583.1"
                     /translation="MKKQLILLLYLFFVTNIFAQISGYVKDKSMQAVPGVNIRWAGTN
                     EGTVSDADGHFRLNTSEDNNILIFSNVAYNPDSIDISKIEKQDRMEVILDDAISLSEV
                     TITKQRLGKIKSRTAVFNVETLNTHELRKAACCNLSESFETNASVDVSYSDAATGAKQ
                     IKLLGLSGTYVQMLTENIPNLRGLSAAYGMGYIPGPWMESIQISKGTASVKNGYEAIA
                     GQINVEYKKPQNSDKVSLNLFTSSTGRTEVNADAAVKLNEKLATSVLLHASDEFLTLD
                     ENKDGYMDLPMVKQYNLANRWYYKNNGYILQAFVRGLSEKRTGGRTDDSYKIGVNTER
                     YEFFVKNGFLFGEDEAKHNHEAGEEHEHHNQSSLGIILNGTIQNQNARYGLKNYDGHQ
                     NNLYANLIYDASFGEYHKISTGASFNADAIDEKLAVNILPENLSRTEYVPGIFAEYTF
                     TRKALTAMAGFRADHNSVYGTFITPRFHIRYNILDHVHLRASAGKGYRSPNVLAENNF
                     LLASNRKLNIAPDLKMENAWNYGFTVHGFIPVFGKELSLMGEWFYTDFNNQVVIDMDT
                     DPHAVNFSNLQGRSYASSFQIEANIEPVKGLTVTLAHRMNDVKTTINENLREKPLTNR
                     TKSLMTLSYQSPLRKWQYDFTTQFNGGGRLPDPDSNRPLWEREFKPFTIMNAQITRNF
                     KTWSVYGGAENITGFVQKNPIIDIQNPFGTDFDASMVWGPMHGRTFYLGFRWALAKSS
                     N"
     gene            26342..27157
                     /locus_tag="PKH58_00755"
     CDS             26342..27157
                     /locus_tag="PKH58_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HNX87584.1"
                     /translation="MNKYLVFSFYLPLILLNSCAKQEMEMKNYSYSHTFHLTEDTTLG
                     GLSISIEAELPVKYHNKEILTSIQQQIVGKMFGEIYTSLPVDSIIPRYVSRLYDDYKI
                     SYGDDLEKYKNLKGPMLDNEIQVEAVSMFLDDKILSYSYERYAFMGGAHGNSNRYFYN
                     FDLSNGHILKESDIFIDNYKSPLTELIKQCIVEDNAEIESVADLNEFDFWEDEIKPNN
                     NFYISDEGIVYVFNPYEIAPYSTGQTEVTLPYNRLKVLLKPDNLISYLYTTTS"
     gene            27172..27711
                     /locus_tag="PKH58_00760"
     CDS             27172..27711
                     /locus_tag="PKH58_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX87585.1"
                     /translation="MSFIELLTNRRSIRRYTDQKVEPEKIQQILTAALMSPSSKRSNP
                     WEFVVVENSDLLLKLSECRPKGSQLLAGSPLGIVVLADTNKTDVWMEDASIAAILIQL
                     EAQDLGLGSCWVQVWGREKEEGVTAEAYIRGLLNIPDNFAVLCIVSIGYRNEDRKPYD
                     SGKLDYIKIHFEEFNNHNR"
     gene            27730..28047
                     /locus_tag="PKH58_00765"
     CDS             27730..28047
                     /locus_tag="PKH58_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNX87586.1"
                     /translation="MKDSRLAWGISLLLFGVMFLLRQLGVFSPEIASVVFDFKNFPLI
                     IGAIFLIWHKNKSVGMVLVVVGLLLRLQEIIHWTKNLSEFVWPLLLIAAGIILLLGVR
                     KGK"
     gene            28087..28854
                     /locus_tag="PKH58_00770"
     CDS             28087..28854
                     /locus_tag="PKH58_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNX87587.1"
                     /translation="MKIVLLGAGNVATHLGARLLQTGADIRQVYSRTDQSAKELGRLL
                     HVPHCSDSNRIDKTADFYIYTIKDNALSEVASKILVPDAIHLHTAGSVSMNIFEGYAN
                     NYGVLYPLQTFSIAKPVDFSKIPLFLEGNNEFTISALTQLAEKLSSVCYFLSSEDRKK
                     IHLSAVFACNFTNYMYQLAEEIVREAGIPFEVLLPLIRETADKVKYISPKDAQTGPAV
                     RNDEQTMLTHISLLKYNENARKIYLTISEQIKQLHNK"
     gene            28851..29333
                     /locus_tag="PKH58_00775"
     CDS             28851..29333
                     /locus_tag="PKH58_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNX87588.1"
                     /translation="MKTAIIYTSVHGTTEKVVGYMANKLHAENPAVIPLTKVRNSDIE
                     NYDRIILGASVHMGVIPKAMKRFCSENLSTLLKKELGLFVCGMEADATQQEAELELAY
                     HGELYNRAKAKAFAGGEFLFEKMNFIQRTIIKKISKVNTSTSDIKYDVVDRFITQMQK
                     "
     gene            29334..30320
                     /locus_tag="PKH58_00780"
     CDS             29334..30320
                     /locus_tag="PKH58_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNX87589.1"
                     /translation="MEAYIRIIRWKNLIFILLIQWLMQYAVVHPMLQTYGFEIYNRDI
                     FNILLYAATILITAGGYVINDYFDVKIDRINRPDRVIVSEKITSKAAMILYQILTGSG
                     VISGLALAILSHSFTLGFIFLLTPGLLWFYSASYKRQFITGNLIVSFSASLVALVVGV
                     LEVSLLKIQFGNLLSQTPIPAQVYGWTGGFAGFAFFCTWIREIIKDMEDIHGDRELEC
                     RTMPIKWGIQKTKYFLYGLIVLTIAGLFHVDYHYIHFDGNLTFRYLLFGVVIPLLALG
                     YLIFRSKTPEDYHQASTFSKLIMLTGVLYSLIFYYLTAKTYHISIFDLFFVK"
     gene            30326..30904
                     /locus_tag="PKH58_00785"
     CDS             30326..30904
                     /locus_tag="PKH58_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNX87590.1"
                     /translation="MLEHLNKYDIILGSQSPRRQELLAGLNINFRVETIYVEEKYPET
                     LVGVDIPMYLAQKKASVYTLEDNTMLITADTIVWHEGTVYGKPNDWTDAKKMLQTLSG
                     KMHQVITGVCIATKRKKKVFHVISDVYFARFTDAEIDYYLNNYEPYDKAGSYGVQEWI
                     GYIGVERIEGSYFNVMGLPVQRLYNELKNWKD"
     gene            30910..31632
                     /locus_tag="PKH58_00790"
     CDS             30910..31632
                     /locus_tag="PKH58_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNX87591.1"
                     /translation="MNKVSVCFSPALYPFYAQKDEIVIIIDIFRATTSICAAFNNGAR
                     SVRTVATTEEAIRLKSEGYPVAAEQNTQRCEFADFGNSPFDFTAEKVNNRDLVFTTTN
                     GTQAVEAALGAEEILIGSFSNHGALLRYCLNAKKDMLLLCSGWKNRFCIEDTLFAGRF
                     ADELIHAGSFVSDNDAVKAARDFWKVAKSDFINYICDTEHYQRLKKNGLENEIEYCLA
                     EDTTSVVPVYDKTIKAFINQRN"
     gene            31636..33027
                     /locus_tag="PKH58_00795"
     CDS             31636..33027
                     /locus_tag="PKH58_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HNX87592.1"
                     /translation="MKLINQNMKIATSLFTALFIFTTGCKTEEKVTLPTVTTYTVTDI
                     TTQSATSGGNITDSKGMTVASRGVCWAKTDNPTINDNTTTDGSGTGDYTSTLTNLTPA
                     TEYYLRAYATNKAGTAYGNTVTFTTQSNGTSTFAKGADIGWLQQMEATGYKFYNDAGI
                     ETDCIQILKDHGINSIRLRTWVNPSNDKASGHCSKDETVAMAVRSKNAGMRVMIDFHY
                     SDSWADPSKQVKPAAWSGHDFTQLQKDVYDYTFEVMTALKNAGVTPEWVQVGNEITPG
                     MLLPDGGSDNAHFPQLVKLINQGYDAVKAVCPTSKVILHIDQGNNNARFRWWFDNAKT
                     YGAKYDIIGLSYYPYWLSDSHPDYTLSIDDLGKNMNDMVTRYGKEVMVVEVGGEDGQD
                     PQLSAAQNAAKIQNTYDMLVAVLQKTRAVPNGKGLGVFYWEPQGARSWSHYALSAWGN
                     DGKPTRALDAFLK"
     gene            33166..33678
                     /locus_tag="PKH58_00800"
     CDS             33166..33678
                     /locus_tag="PKH58_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNX87593.1"
                     /translation="MLNPKISKLLNDQINKEFYSSYLYLDMSNYYYDNNLKGFGNWFA
                     IQTQEEYAHAMLFLKYMQNNGEKITLEAIAAPGLKYANLKAPLEEALKHEYFITSSIN
                     DIYAAAYDVKDFRTMQFLDWFVKEQGEEEQNTDDLCKRFDLFGSDPKSLYLLDNELST
                     RVFNPPSLVI"
     gene            complement(33905..36706)
                     /locus_tag="PKH58_00805"
     CDS             complement(33905..36706)
                     /locus_tag="PKH58_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HNX87594.1"
                     /translation="MSNKIFLLAFVTIAGINATPLFSQTTSASSYFSVQPASDRTVIF
                     NVADPGVSKPIIWGLDLAWLSESNIRRGIAFMSPRKVDVVRASFQPTYPLVNGDLQTA
                     QIDSLNIRLNLIDLTGTETKVALNCDHPRVDSWYIGNAANWAQLIDVTTRRVQERGRT
                     VVSVAPFNEPDFGWGQYSGSKSNGMSDFKNIAGELRKNPRFDNIRISGGNTLNCDEAL
                     SWYNYLKDRLEEGNTHQLAGSFDNYANFHKSVAANGDHGTNDELHNVMEAMVGVEYGM
                     KTGIWWGTAEYARGEFVKASDGVRLGYAEHRPNWTAASVYRSPDGKIQAFGGTSERQA
                     ATTTYRFFSKTKDVYFDGYGPQREYTMVLPGGTGYQKGQTNAERVMNITWGDDIQPVI
                     NGRYVLVNRNSGKVMEVTFGSTSSGANVWQNSFIPDATYQQWNVTPVDSRIGGDFSYF
                     TITSVKSSKSLDIYNWSLDNTGNIVQWDVANGANQQWYLEYAEDGWFYIRSRHSAKCI
                     EVANASMTNGANVYQWEKDGDRNQQWRFIPVGAPVEFVSPAAPASLTATPNAESVKLE
                     WTASADTDVAGYNIYRKDSPEGKFETIARNIKNTSFVDNTTTENGQYFYAVRAVDNSL
                     NRSVYSNEASAITTGANDLVASLQFENNISDSTVNLNHAASSGSISYTTGKTGANAIS
                     LNGTNAFLQLPPNIANQQEITIAAWVYCRGGTVAQRIFDFGNSQNEYMYLTPNNGNGQ
                     LRFGIKNGGDEQQLNATSLAGSKWIHVAVTLATDTARLYVNGIKVSESNTMTIKPLDF
                     KPALNYIGRSQFSYPLFNGNIDDFRVYNYALTPEGIAEIAGILSGVEDLKSVSRQAIS
                     IFPVPAEKVLNIKFNEKISAEKSEISVLDVSGNIHFSQRLTEYDNVSLDVSDFPSGIY
                     ILKLSAGSEIFVKKFVVNH"
     gene            36904..37692
                     /gene="ygiD"
                     /locus_tag="PKH58_00810"
     CDS             36904..37692
                     /gene="ygiD"
                     /locus_tag="PKH58_00810"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNX87595.1"
                     /translation="MNYNTQKMPVLFVGHGNPMNAIEDNSFTQTWHQIGKGIPRPTTI
                     LSISAHWETEGTYFTAMQTPRSVHDFGGFPRPLYNVVYPAPGNPELAEQIKSVIKGQV
                     IELDELEWGLDHGTWSVLVHMFPQADIPVIQMSLNRNMTPRQHYELAQEMSYLREQGV
                     LIMGSGNIVHNLREVDWHNPYSAYDWALDVNDKIKNQLRNHDHDSLIDFRKQGLEFRM
                     AIPTAEHYLPLLYLIGLQSDSDEISFFNDKISMGSLSMTGVMLK"
     gene            38239..39435
                     /locus_tag="PKH58_00815"
     CDS             38239..39435
                     /locus_tag="PKH58_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HNX87596.1"
                     /translation="MITPISTPASTERIQVVDALRGFAIAAILLLHNLEHFDFWYTPQ
                     GFPSWLVNLDKGIWDTMFFLFSGKAYGIFALLFGFTFYIMQHNQAKKGKDFRLRFAWR
                     LLLLLGFGVINSAFYQGDILTIYALLGITLIPVAKLSNKAVLIIACLLIFQPWEWGKF
                     LIALTHPAQDLADPASWAYFGKMSEYITGNSFIKTIAGNLTNGKAGVYLWTWEAGRVF
                     QTPGLFMIGMLLGRTGKFIFSGETKRFWTKILIISSVAFVPLYLLGIGLTPGNIPNEA
                     LLRPLSLIIKSWSNIAFMFVLIAGFVLLFQKSTIQRVLNVFSVLGKMSLSNYIIQSIL
                     GATIYYGFGLGLYQYTGASMCLIIGLALMILQWHFCKYWMRSHRYGPLEGIWHKLTWI
                     NSENNK"
     gene            complement(39451..40851)
                     /locus_tag="PKH58_00820"
     CDS             complement(39451..40851)
                     /locus_tag="PKH58_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX87597.1"
                     /translation="MKSTPLTDKNINKTLLLVATMATQFFNPFMGAAVNVALKKIGTD
                     FSMTAIGLSWVSMSYLLASIIFLVPFGKLGDTWGRTKMFLYGTVFFAITTLLCAFVPN
                     STTFIIIRFMQGMASAMMISTLMAIVISAYPPEKRGKMIGLNVSAVYVGSSLAPMIGG
                     LITDAFSWRSIFLINAVVSTLIAILIIWKLNRELSETIKEKFDYKGTILYMISVSMLM
                     YGLSKLPHQLAIVLTIAGIAGLFFFAKLELKTQFPVLNIRLFTENRVFALSNLSAIIN
                     YAATFSISFMLSLYLQYVKGMEAREAGLVLVAQPVLMAIVSTFSGRLSDKMNPRNLAA
                     LGMGISAIGLLILSFISSETTIIYIIIGLIVLGAGFGLFSSPNTNVVMGSVDKKIYGT
                     ASAILGTMRNTGMMFSMAIASLTLHLFLGNAQIIASNIPQLILSTKTVFALFTMLCFI
                     GVFTSLVKVEFRKTGK"
     gene            41344..42078
                     /locus_tag="PKH58_00825"
     CDS             41344..42078
                     /locus_tag="PKH58_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNX87598.1"
                     /translation="MKNGVLMTAEQYYNSLPAVIRKKKRNPLQTEIPNFYLVSGFTHP
                     MLPVITDDEMTLKQWGLIPEWTTDRHQADELQNMTLNAVGETVFEKPSFRRNILSRRC
                     LLPVSGFYEWREFRNTKYPYFVHPKDASGFLLGSLYDRWTDPLTGEHHDTFSIISTPA
                     NPLMEVIHNVKKRMPLILDDDAADRWLNPATSPDELKTLIKPYDENKMDAYTISKAAG
                     NPNFDRNIPEILNPVKYSELDTPTLF"
     gene            42373..42828
                     /locus_tag="PKH58_00830"
     CDS             42373..42828
                     /locus_tag="PKH58_00830"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNX87599.1"
                     /translation="MPSKKLIDFLNENQIKFVLIHHSRAYTANEVAHSAHIHGQELAK
                     TVILKIDGKLAMAVRPASGLVNTRLLKEELGASMVEVAAEHEFANVFPECEVGAEPPF
                     GNLYGMNVYVSDSLAQEPEIAFNAGTHTELLRMKYEDFERLVNPEVLRF"
     gene            complement(42952..43545)
                     /locus_tag="PKH58_00835"
     CDS             complement(42952..43545)
                     /locus_tag="PKH58_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HNX87600.1"
                     /translation="MRNLILILIPIMYLQALTVKAQEKGIFTDNRDGKIYKTTTIGTQ
                     TWMLENLAYKTDAGCWAYDNNPKNVAVYGYLYDYAAAIKACPDGWHIPSDEEWKILIK
                     SQGDHRFAGDKLKTITWKKLESEPNNKSGFSALPGGEYDADAHKFWGIEEIASWWSAT
                     ALNNYTYYVYCLHYDASDVSQNQDFDTNAHSVRCVKN"
     gene            complement(43592..44008)
                     /locus_tag="PKH58_00840"
     CDS             complement(43592..44008)
                     /locus_tag="PKH58_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87601.1"
                     /translation="MEKIHQTKVHSERLQLSVLAIVIIVFIIAFLLLNRSFIVSQCSV
                     RPLGIALLLITFEYNDQLLHPIISNLTNHTPVLMLLIFVSVASLMIPLHYKLEKWTKY
                     KQTEKNRLIRLSKAKRIIMEAEEDSGKKQTKRLLNE"
     gene            complement(44151..44594)
                     /locus_tag="PKH58_00845"
     CDS             complement(44151..44594)
                     /locus_tag="PKH58_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNX87602.1"
                     /translation="MSLARFTQSPLDRFFDNDLLDWSNRNFSKTNTTLPSVNIRENKE
                     EFLIDVAVPGFDKNDFKIEVNNDVLTVSSEKQEESETKDEKGKHGERYTKREFSYQAF
                     IRSFTLPDDADADRVTASYDKGILTVLVPKKEETKPRTSRMIEIK"
     gene            complement(44748..44963)
                     /locus_tag="PKH58_00850"
     CDS             complement(44748..44963)
                     /locus_tag="PKH58_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87603.1"
                     /translation="MKTKKVSIVEHQKDADIKVFFSDKEHEQKNHHLLTESKLTVRNV
                     HPDLRIIIEEHHNRAIIRKLRKNIPKK"
     gene            complement(44953..45294)
                     /locus_tag="PKH58_00855"
     CDS             complement(44953..45294)
                     /locus_tag="PKH58_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87604.1"
                     /translation="MNGITGYITKEGFGIYVHDKITRATLTEECKTVQQFIDTYGLSA
                     LQIGLFLYSEAKNEFLKVTQVCVNFKDRCIGVYLEKTENHITSLHNDPITEKETTKKS
                     GHKVHGKNHEN"
     gene            complement(45398..46042)
                     /locus_tag="PKH58_00860"
     CDS             complement(45398..46042)
                     /locus_tag="PKH58_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNX87605.1"
                     /translation="MNQNAPLIVTISREFGSGGAYIGQQIAKKLNMYYADYEIVAKAA
                     EYFSANIVDVASQEERVEPLWKSLWNFYGTAVPNVYVPTRRIFVPTSQELFNEQSQII
                     RKIAAEHSAVIIGRGGFYVLRDFPNRIDICLHADIEFRNRRLQGLYNISEEEANNMIK
                     QTDKERALYISTFTGMDWNNSKNFDLTLNTGKLGINKSLELILEFIRLRSDITL"
BASE COUNT        13793 a         9547 c         8768 g        14343 t
ORIGIN      
        1 atgcagacac catcttttat agtcacctcg gatgccggta ccggaattgg atataccggt
       61 tttcgcatac gcggtacaga tgccaaccgc accaatgtta ccatcaacgg aatacccttc
      121 aacgatgcag agtctcacag cgtatatttt gtggacttac ccgacttggc atcttcactt
      181 cattcggtac aggtgcaacg tggagtaggt acttccacca atggtgcccc ggcattcgga
      241 gccagcatca atttacaaac cgaaaaaatc aataaaaatc catattccga aatcggcact
      301 tcattcggtt cgttcagtac actgaaaaac acggtaaaag caggtacagg tctgattaac
      361 aattatgcag tcgatgtaag gctatcaaac gtcaattcga acggttttat tgaccgggca
      421 tcagttgatt tgaaatctta ctatgtttct gccggatatt atggtgaaaa aacgatatta
      481 aaatttctga cttttggtgg aaaagaaaaa acctatcagg cctggaatgg tgtagatctg
      541 gattactttc cgcgtaacta caatgaactt ggaaaatata cagataagaa cggaaacatt
      601 caattttatg acaatcagac cgacaattac ggtcaatatc attatcagct tcatttttca
      661 catattttga aaccgtcaat ttatttaaat gcagcattac attatacgcg tggcattggt
      721 tattacgaag aatacaaaac agaccggaaa tatgctgaat atggtctgac gcctgcattt
      781 tcaggtgaaa aattatttga taaaacagat ctggtacgtc agaaatggct cgataatcat
      841 ttcggaggtg ctactttttc ggtcaatttt aataaagaaa attattcgct tatactgggc
      901 ggggcagcca acaagtacct gggtgaccat tatggaaaag tgatctgggt gagaaatgcg
      961 aacaatctga acgtagatca cctgtactat ttcaataaaa gcacaaaaaa tgatttcaat
     1021 atctatggaa aattcaatta tcagattttt gataacataa tgacatacat agatttacaa
     1081 taccgcaatt tagctctgga catgagcggt gatgatgata aattcaattc tgaaaccggt
     1141 aaaatgcgtg tattgtcaaa ccagaaaacg ttcagtttct tcaatccaaa atttggagtg
     1201 aattacaaaa ttaacactgc agataacgca tttatttcgt ttgcgatagc taaccgagaa
     1261 ccaaacagaa acaactacac cgatgccgga cccaacgaac agccaaccag cgaacgcttg
     1321 tacgatactg aattgggctt cagtcatcaa tcaacagatt tcagtttcgg cgcaaatatt
     1381 tactacatga aatataagaa ccagcttatt ctgaacggaa aaatgagcga aataggcgaa
     1441 cttctcactt caaatatccc tgacagttac agaactggtc tcgaactgac cggaggtata
     1501 aaaataaccc gaggtttacg ctgggacgga aatttgaatt tcagccggaa taaaataaaa
     1561 aaattcaccg aatttgcgga cgaatacgat gccgactgga actggaccgg aattaaacaa
     1621 aacttctatg caaacaccaa tatttcttac tcacctgatg tggtggcaaa cagtattttc
     1681 agctatacgt ttcatggctt cgaaactgca ttatatacaa cctatgtagg cagacagtat
     1741 ctggataaca cttccgacgt taaacgttcc attgatgctt attttgttaa taatctccgg
     1801 ctgggctata cactgaaact gaaaaaagtc aaatccattg actttaatct acagataaac
     1861 aacctgttta acacagaata tgaaacaaac ggttacaatt ggtacagcta ttacatggat
     1921 ggaaaaagga tcaatgagaa aagatatttt cctcaagcag gaataaatta cctggcatca
     1981 atagccttca aattctaact taatcaaaaa tcccttttca atccgaagag ggatttttta
     2041 tttatacaac agaaaaatac agggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnatttcc cactcgtaca ttttttgttc gagttcccgt tgtttttttt gtaaaagaat
     2221 aataaaatcc ggatcagaag ctttttgcgg attttcgagc tggtgttgca tatccgatat
     2281 ttcattttca agattttcca cggaattttc agcttcgtcc acctcttttt cggcttttcg
     2341 tattttttta ttcagctctt ttcgagcctc ataggatagt ttattttcag aaacttcttt
     2401 ttcagcagga gtttcattcg ttgttacagg cgattgtttc agtcccagtt cattcaggtt
     2461 ttcaattttc tttttctgca ggaattcata gatgccaccc aggtgttcac gcaccttttt
     2521 attgccgaat tcatatactt ttgttaccag gccatccaaa aattcgcggt cgtgcgaaac
     2581 aattactgct gttccgtcaa atgcttttat agcttctttc aacacttcct tggatgccat
     2641 atccaggtga ttggttggtt cgtcgagtat cagcagattg acaggttcaa gcaatagtct
     2701 tatcattgcc agacggcttc gctctcctcc cgaaagaacc ttcacttttt tgtcggacgc
     2761 tggtccccca aacataaaag cacccagaat atccctgatt ttagtacgga tatcacctgt
     2821 cgccacataa tcaatcgttt gaaatactgt cagattttca tccaggagcg aagcctggtt
     2881 ttgagcaaaa tagccaattt ttacattatg acccagtttg aggatgcctg agtacgttat
     2941 ttcactcaac atacatttaa cgagcgtact cttcccttct ccatttttac caacaaaggc
     3001 gaccttttct ccccggttta caatcattcc aatattatcg agtaccagat gattaccgta
     3061 tgatttcgac agatgttcga tttcgagagg tatcgaaccg gaacgcggag aaggtggaaa
     3121 tttcagacta agccgtgagg tatcttccag atcaacttca aggcgttcta ttttttcaag
     3181 ctgtttgata cgcgactgta cctgaacagc ttttgtagct ttatagcgaa agcgttcgat
     3241 aaaatcttct gtttcctgaa ttgccttttg ctgattttcg taagccctca cctgctgctc
     3301 gtgtcgttct ttgcgcaatt caacgtattt tgaatagttt acgttgtaat cataaatccg
     3361 acccagggat atttcgatgg ttcggtttgt tacagcatct ataaaagcct tatcgtgcga
     3421 cacgagcaaa actgcgctac ctgtacttgc caaaaagctt tcaagccatt gaatggattc
     3481 tatgtctaag tgattggttg gctcatcgag cagcagcaca tccggatttt gtaacagaat
     3541 ttttgccagt tcaattcgca tccgccatcc gccgctaaat tcggcacact gacggtcgaa
     3601 atcggttctc agaaatccga gtcccagtaa ggtcttttca atctctgcat aaaaatttcc
     3661 gctgttgatt atttgcaaat gttcatttgc gtgactcagc ttatcgatta aaaccgtata
     3721 ttccttgctt tcataatcgg tacgttcgga aagttcggcg gtatatctgt tgatatcttc
     3781 ctgcagtttc agaatatgct caaatgcttt ctcagtttcc tgcatcactg tagtaccttc
     3841 gttgtgaatc atatgctgag gcaggtaccc gacagttatg tcagatgctg aaatcacttt
     3901 tccgcgggtc ggcagaattt ttccggcaat cagttttagg agtgtggttt ttccggctcc
     3961 gttacggcca actaatgcta ttttatcttt ttggttaatc agaaaaccaa cattgtcgaa
     4021 cagaggtgat ccgccaaatt ctacagtaag ttgttctatt gaaatcatgg cttttcagtc
     4081 aggtctttat tctgtttcaa ctcaacaagc tgctcggttt ttggcatttt tttacgatcc
     4141 acaagtatcc atagatattt tgtcaagata aggcaaaaca caaaatcaat cgcaacaagt
     4201 actccattgg gtatatttct gaaaattagc cctacaataa taaagccgat agtaataccc
     4261 attaatacaa atgagacatc cttatgtttt aaacccaggc ttaataagag gtgatgaaca
     4321 tgatttttgt cagcccgaaa cggagaatag ccctttttca ctctggtaac cattactctc
     4381 actgtatcga acaatggcac aataagcagg gatatagcta cagcaggagc tgcttttata
     4441 aataattgag gtggaacatc atgtttcgca ttcatttcaa gaaatgaaaa cacaaataaa
     4501 ttgatcatat atcccagtaa aagcgaaccg gagtcgccca taaaaatctt tcttttattg
     4561 gagaaaacat tgtaatagaa aaatactact aaagaaccaa acatagcgaa ggaagaaaca
     4621 gaaagttcaa aatgtccgct aagcagaaaa taaacagcaa aaaacaacga atacagaatg
     4681 cctaaacccg atgccagtcc atccacgccg tcaatcaaat tcagtgaatt cacaattaca
     4741 ataaaaacga aaaatgatat cacatagcta aagaacagcg gtagttcgta tattcccaga
     4801 aaaccgtgaa agttagaaat ccgtaaatcg gcaagaataa tgagaaagaa tgctgctatc
     4861 agttcaccga ttagtttcca gctggcttta atatcaatta aatcatctat aaagcctaca
     4921 atcaggataa taaatagtcc caggatagaa aactgaattt ccgaaaacac attgtatgaa
     4981 aataaaaata cagtaaacag aaaagaaaca aaaatattaa taccaccaat gtttggtata
     5041 tcaccgttgt gcgatgtgcg tttattcgga cttactacaa agttgtattt tcgggctacc
     5101 tcaattacaa ggggcataaa aagatacccg attgtaaatg aaattattga tacaaaatat
     5161 ggatgtgttt tgaaaaatgg taaaatctgg ttcattctgc tattatattt tcaacatcaa
     5221 gtctttcata ctgagagtta ttgctgataa attccggtac tattgacttc atctgcgaga
     5281 cggttaaaaa attctttcct aagtaactgt atgtgatcaa ctcatttatt ttctgtgaaa
     5341 tatcgtcaaa gtcatattcc cgtacagttg caatcataat tttgggatga tgggttttct
     5401 gagtaatttc ttttttattt aaaagttctt catataattt ctcaccgggg cgtaatcctg
     5461 taaatgaaat tggtatatcc acatcaggaa tgtaaccagc taaccttatc atcttttttg
     5521 ccagatcgag aatttttacc ggctcaccca tatcaaaaat atagatttct ccaccattac
     5581 ccattgttcc tgcttcgagt acgagtaagc aggcttcagg aatagtcatg aaatagcgta
     5641 taatatccgg atgagtaacg gtaacaggtc ctcctttctc tatctgctgt ttgaaatgag
     5701 gtattacaga accatttgag ccaagtacgt tgccaaaacg tgtagtaata aatctggtta
     5761 cagccccgtc ttccttttcc tgttttttat tcagcgactg aacataaatt tcagcaattc
     5821 gtttggaagc tcccatcaca ttggtaggat tgacggcttt atcggtagat accattacaa
     5881 acctcgaaac tccatatttc aatgacaaat cagcaatatt tttagttcct aatacattgt
     5941 cctgaatact ttcggtcggg ttgtcttcca ttaatggaac atgcttgtat gcggcagcat
     6001 ggaatacaat atcgggtcgg taaatttcca tcatgctttt catccgttca agatttctga
     6061 tatcaccaag gtatgaacta aagtcctgtt ttggatattg ttctttcagt ttcagaatta
     6121 aattatgcat cggagtttcg gcctgatcga caagaactat taactgaggt ttgaatgagg
     6181 ttaattgacg tgcaatttcg ctcccgattg aacctgctgc tcctgttatc agtataactt
     6241 tattgttgat gacacttgaa atattttcag tcgaaatttt aatctgaggt cgctccagca
     6301 gatcttcaat ctgtatagat ttgatctgct ttggacttgg catcttgtca ccatcccata
     6361 tactcatcgg tggggtaatc aacaccgaca ttttattatt caaaaaaata tccaaatcaa
     6421 tctgaggatt gatcgtttga agcttcagag gagagacaat aattgctttt gctttttttg
     6481 agatgatttt ttttacattt tcctctgttt tcttataaac ttttactccc attacaattt
     6541 tttcgactga atttttgtca tcatcgacaa atccaacaag tttatattta acgttttcat
     6601 tggtgcgaat catttttgca atggcaatac cagctgattt tgttccatat atcattattg
     6661 gtatcgaaat tcctgaattt tgagatatgt aatcatatat tgtttttact gccagtctga
     6721 taataaacag caaaaagaat gatattacgc tgtaaaagag gattgattcg taataaaaaa
     6781 ggtgaatact aagaaaggag ataaacaata aattaagtat tacaagtaaa accgcattcg
     6841 agaatacagc cagaagcagt tttgcagcat cgacataacc tgaatacctc agtatgccgg
     6901 aatatgtatg aaaaaaccaa aaaaatatga cctgaaataa taataacaga cttaatcggc
     6961 ttgtaaaggg aagagccagc acctgattat gagtaacaga caaattaaca gcaatcagat
     7021 aggaaataac aaacgcaata atacatacaa caatatcaac agataatact aaccatcgcg
     7081 gcagatacct taatgacata aaatgagaaa atatatctga ttttttaata aagttccagg
     7141 gaattttcat atttgattat atatcaaaac taactcaaaa ttaattcaca aagataatat
     7201 aacttcaaaa acttttacta acatttaaca taaatcaaaa tgtatagaag ccctgtcgta
     7261 ctgatttgga tttcatttga attttttcct gagatttttc ggcagataaa aagtattgtt
     7321 tttatcgata taaactacta ccgaaccgat gtcaaacggt ttttcgttaa atttccccac
     7381 agaactaaat gccggaattt caagcactcc tgattcatat ttcacaatta acttaatcgg
     7441 tttatcctgt tgattttcaa tgcggtattc catgccggca aatacatcgg tatgttttgc
     7501 aaacatttca tttgcttgct cttttaacga agtcttcaag ccggcagctt tgaacagata
     7561 gttatttact tcgttgttac ggatattacc cgttggcctg tggtttttcg ggtgatatac
     7621 cgccaggaaa acatcctcac cggtatgtcc acttgatgta aacccaaaat tgctgtgtga
     7681 attcataatt ttggttatga aataagccaa attctgaaca tttgcagcat tcatataatt
     7741 atccactttg taattattag ctcctttcaa tgaatttaat tcatcctcag ttatatcaat
     7801 tcccgtatat attttaaaga cttgttttag actgtcaggt tttgtactta gcagtatttt
     7861 ttctaatccg acggttgttc ttttgtactt tgaaaatgtt ccaaagaggt cttccagtgt
     7921 cattttcgtg taacttttac tcattccgtt tcttcctatt gaaaacccac tgttgccatg
     7981 atcggcaagg actacaacag tcgtattttt gtcttttcgg gcaaaatcaa ttgctttccc
     8041 gattgcttcg tcaaatgcaa ggtactcgct gataatcccg gcaggatcgt tggcatgagc
     8101 agcataatca attttacttc cttctaccat cagaaaaaaa ccttttttat ttttattgag
     8161 tacttccaga gctttcgaag tcatttgcgc cagtgatgga acattcattg tatctctgtc
     8221 caaatcgtaa ggtatttcac gctgtccaaa caatgcccac actcgttcac cattaaactt
     8281 catcaaggcg tctttatcat ctttgatata ggtagtaccg gtattactga aatgttgctt
     8341 catatcctca gttacataat catttcctcc tccaaacatt acttccaggt tattataagc
     8401 catctgcggg gcaagtactt tgtaatcgct tctgttataa aaatgtgaag cacaatctgc
     8461 cggtgtagca tgtggaaatt cgcaggttac aacaagtccg gctgctttat tctgctcttt
     8521 ttgcatagct tcaaggatag ttacaagtgg ttgataggcg ctatccggat tcaatctaat
     8581 cagatcatta gccgggtcag caaccggata tatagagata tttgaagcct gcgctgcaat
     8641 tccagtcatg taacacgaag tagtaggcgc tgagtcaccg atgggtgcat tggacgaaaa
     8701 cgtagaaacc gtcccacata gccagggatc aatatttagt ttattacctg catttcgata
     8761 tattttcgtc caacgagatg ctgtcagaac actcggtgaa gtcccgtcag caatcatcaa
     8821 aattatattt ttagttggtt gtattttctg ggcatagttc aaaagactaa acaacaatac
     8881 acatgttatt aaacaaattc ttttcatcat tacttttcgt tattttattt gtaatatgtt
     8941 tttatcgttt tacttatcaa atctcccact ccaattcgat atccttcgga ataaaaaatg
     9001 atttggccct tagaatcgac aatataaatc agtggataat tatcacgaaa tgactgattg
     9061 gcacttttca aaaactcatc cataattttt tcaccgttgt tcaaaataaa tgtgctgttt
     9121 tcaggaagat ttttatatct atccggcgtg aaattttttc ctgttttgtc agtaggtact
     9181 acgaaaacaa attttccact ccatttatca aattgcgttt tataatccgg cagctcattc
     9241 attatgtgtt tggttggttc acgatcgggt tccagaaaac agattaccat tccacgcggg
     9301 tcataataat cggaaggcaa tatggaagtc ttaagcacgc cgtatgtctt gttttccacc
     9361 tccaattctc tgattgtaag ctctttgaca acctgtttgc ttttacttac cgaaaaatat
     9421 tcgtttcgcg caagtacatt tccgttcgca tacctgttac ctgttgtcag gcaataataa
     9481 ccttcttcga gttcaagttg aaccggaaat ttctcaaccc gcttgtctcc ttcaaaatca
     9541 agcgaatgaa aatatccatc actaaatttt gaaatggtat aataagtcca gtattgaggg
     9601 accaaatcat cctgagaatg aaaattgatg gtcagatttc cggttggcag tttattttta
     9661 tttggttcaa gtactacctt ttcccatcgt ccctttacat actgacttat ttcgctggtt
     9721 gccggatcaa taatcgttgg tatattgagg cttctgcaca aagcaacaaa aaacactgtt
     9781 tccgaattaa tgtcgcactg tttcaattta taaactccga caggtgaaat cagacatccg
     9841 gaataatttt catcttctac aattttaata ttctttttta tccattccct gattaagtca
     9901 acagttacgt ttgacggaaa aatttcttta aattcttttt gtaaaaacgg tcgccattca
     9961 cggatcaact cattggaaat acgcggagaa agaactccct ttacaaaaac atcatcaggt
    10021 aaattatctg tattttttga atgcaacaga tgactctcca aaactcgaga aggcgtatct
    10081 cgtaagtcct tatcgctcag cgtagatagt aagtcaagta ccagaggatt gctggcattt
    10141 cgggtgagat aagctgtaat ttccgaataa ttgccttcgc ttttggctat gtattctgtt
    10201 accttatccg gatttaatcc taacttacgg gcaagcaagc gcgcactgtc aggtttcaaa
    10261 aaggtcgata aataaactga tcggattgaa tcctcgaact gcagacgttt gttgttgatg
    10321 gctgtaattg cagatgtatc tttatggaaa gtggcctgat tgaccggtgg tttgatatcc
    10381 cattcttcct gatattcctt ccctggcgtt ttgtcaagct gaaccaccac tttgtcatcg
    10441 tcacttaccg atactttctt atatccatat ctgtcatctt ttgcagccca aacaaccata
    10501 tcgccaagac cggttgttac agaggcattt ccttttatat ccgtaatttg ctcatataca
    10561 ggataatatt cagcatagtt gtagataaga aatttcactt ttgctccgct tactgcattg
    10621 ttcttttcat caactacagt aacatttagt tttttggtag tagtataatt ttcaagaaca
    10681 ttaattttgg tgtatagcgg atattctgtt ttgctttcag gaccgtaata ttttccgaag
    10741 acattggtat gaaccatcat agcacgcttt gccggatatg taaaccaggc tttattgagt
    10801 tcagcttccg gttcgcaggc acccatgaag taccacttcc cgtcagccca aacctccacc
    10861 caggcatgat tatcatcggt atgtgcccag cggggagtat agcattgccg ggctggaata
    10921 cccactgcac gcaacgcggc aacagtaaag gtggattcct ctccacaacg gccaaaaccc
    10981 gtccgaactg ttgccaatgg tgatgatgta cggctatcgg caggacgata agtaactttt
    11041 tcgtgacacc aatggttcac ttccagtgcc gcatctttca tattcatatt ctttattctg
    11101 tctttcagct cattgaaaaa cacaatccgg gcagtatcaa ggttttcatt atttactctc
    11161 ggaggtaaca caaaatgacg gaacaattcc tcgggtactt tatctcccca actgaaaaaa
    11221 tcacgggcat caaaagctgt tttaatttgc cggtagaaaa aatccccgtc gtaatcaccc
    11281 aaatcattga gtgacatata cgcatacaga aattgtaaag cttctatttc ctgaattttc
    11341 aaatcatctt tgtcaaaaac tgcaaaaagt tcatcctttc ttccggtaag ttgcaatttt
    11401 cgtttttcaa actgcttatg tacttcactg cgatatcggg tatctgttat gaagtgacta
    11461 ttattccggc aggaccacaa tgaaataatc agaactacaa aaaacagtga atataacttt
    11521 ttcatagcta ttacattttt attttattac cagcaagttc gctgataaat gtatgacaga
    11581 ttagaatatc tttctgtaaa tatggcagta cggagtgccg tttttcttat cgtaatactc
    11641 ctgatgataa tcttccgctt cccagaattc gccggccgct tgaatctttg tggcaacttt
    11701 gtagcctttt tcagtgagaa tttttatgta cttttcagct gtagattttt gttcgggcga
    11761 ggtataaaaa attaccgaac gatactgatc acctatatcg ggtccctgtc cgcctatctg
    11821 agtaaaatca tgcgtttcga agaataattt tatcagtttt tcgtaggaag tttcacccgg
    11881 actgaacact acttcggttg tctctgcatg tccagtggtt ccggtgcata cttctttata
    11941 agtcggattt tgggtatgtc cgcccatata tccaacagta gttgcacgca ctccggcagc
    12001 tttcataaaa tgatactctg tcccccagaa gcatccggag gcaaaatagg ctgtgtccgt
    12061 tgttttttta ttttcagtta cgaatttcat ggaaattgag tttacacaat ggcgtgtatt
    12121 tttcgatgta aacccttcgc cttcaaacac atgccccaaa tgcgcaccac acgaagcaca
    12181 gataatttcc gttcgacgtc cgtcagcatc cggaacgcgt tttacagctc cttcaatttc
    12241 atcgtcaaag cttggccaac cgcatcctga ttcaaacttg tctgatgacc ggtacaaggg
    12301 tgcatcgcac cgtttgcaaa tatatgttcc gctttctttg ttatggagat attctcctgt
    12361 aaatggtctt tcggttgctt tatgaacaat tacgttttcc tcttccggag taagttgatt
    12421 gaatttcatg atttttgaac ttttagtttg accgcacgct gaaaaaacaa aaaagaacaa
    12481 gagtaaaaac acttgccggc tgttgttgct gttaaataag taatgtataa ctttcatgtc
    12541 atttcggatt tatcagacag aaaacaattt ctgagtgttt ttgttccaaa atcaatccgt
    12601 taaataacca aaagtcaact tttcatattc aggaaaatga attatctcag tctgtccatc
    12661 gattttacca tattttcatc ctttgcaata ccacgcatag ccagataatt gaatatcagc
    12721 gcaacaagcg aaaggactgc cggtattttc aatgatattt ctgtaccagt cagtttatgt
    12781 tttcccatga aaataacgac agcaagcagg atggtataaa tcagtataag aatgaaattg
    12841 atggtgcaaa gacgaaactg ctgttttcgg tttttatatt taaaaatagt catcaatgtg
    12901 actactaaaa ttattccgaa gacaaatgtc gtaggattgg tactgacagc cgagaggaat
    12961 gaaccatccg gattgagttg aacaaaacca cgcatatcaa gctgatatga tatattttta
    13021 accgcatcga aatactcggc taccggcagt gctgctgtta aagtcatgag cacagctaca
    13081 agcacgaggt aaacggtttg aattctttgt atcatttttt attacaatta tttatttttc
    13141 ttttttccac tgctgatcgc gcacttgata gatgcgttca ataatttcga ctaccttcag
    13201 accttcgagc acgttggttg taattgaacc tttgtccgtg agtttttcca ccacattttc
    13261 aatcacataa tggtgattct gagccgaacc tttgtaaaga ccgtagtcgt ttggcggatt
    13321 tgatggtgca agttctggca tcacataatc cttcacatga caatattcca cctcattcat
    13381 atactgtccg gcaaccttga tggtgccttt ttctccgatg atggtgatgc tgctttcgag
    13441 attagtatcc caaactgatg tggaatagtt cagacaaccc atacctccgt tgataaaatc
    13501 aaatgttacc acaccgctgt cttcaaaatc agtcagattt ttatgattga aatcggcaaa
    13561 attgccacga atgttggtaa tgtcgccaaa cagccagtac atgatatcaa taaaatgaga
    13621 aaactgtgta aagagcgtgc caccgtccag ttgttcatca ccgtgccagt tacctttttt
    13681 gtaatagcga tcgtcgcggt tccagtaaca atcgagtttt accataaaga ttttaccaag
    13741 caaacgctga tccattatct cgcgaagcca taccgaaggg ggtgaataac ggttttgcat
    13801 aacacagaat acgtgtcgcg acatctcaag tgatttgaaa acaatacgtt cggcatcttt
    13861 tttattgagt gctatcggtt tctcgagtac tacgtgcttg cgggctttca gtgcctttac
    13921 agcataatcg gcatgatacc cgttgggtgt acagatatta attacatcga tatccagttc
    13981 tgagtcgagt aagctatcta tatcgttaaa aaaaggctcc tgaatatcgg cgagtcctaa
    14041 ttgctctttt ggtaaaacat cacaaacggc taccagttcg gcatccccgt tacgtctgat
    14101 catttcggca tgacgtttac caatatgtcc ctgccctatt actgcaaatt ttatcatatt
    14161 ctttttctta taatttattg ttattctgat agtaatttta caaagccatc gttcagttta
    14221 tacttttctc cggtttcggg acaggtagct agcccattat cgtcaaagct tagtttctga
    14281 ccatttcggc ttacccaacc tgtttgtctg gccggattgc ctatgactaa agcaaagggt
    14341 ttgacttctt ttgtgattac cgcaccggct cctatcaacg catactcacc gatttcattt
    14401 ccacacacta ttgttgcatt ggctcctatg cttgcacctt tgcgtacaat agtttgagca
    14461 tattcattct tgcggttgac atggctgcgt ggattaatca cattggtaaa aaccatggaa
    14521 gggcctaaaa aaacatcgtc ttcgcagatt actccggtgt aaaccgagac attgttctgt
    14581 atctttacat tacgtccgag tacaaccttt ggtgatatca cgacattctg gccaatgttg
    14641 cattgctcac caataacaca ttcggacatg acatgcgaaa aatgccagat tttagtgcca
    14701 tcgccgataa cgcaattctc atcaataaca gctgtttcgt gtgcaaaata attcatttta
    14761 tcgtttttta ttgctgatta tattcagcta aaatgttatg aaataaaatt caggatactt
    14821 ttcgtaatat actcaagttg ttctccgtcc agttctgtat gcatcggaag tgacaaaaca
    14881 gttgagcaca gcatttctgc aacaggaaaa tcaccttctt tatatcgtga atttttatac
    14941 gctttctgca tatgcaacgg tacaggatag taaaccattg ccggaatacc cagttctgaa
    15001 aggtgtttct gtaatgccgt gcggtcaaca ccattaagtt tcagagtgta ttgatgaaaa
    15061 acatgtgtgg ttttttcatt tctaaacgga attgttattt tttcacaccc cgaaaatgct
    15121 ttatcataat aatcagctgc ctgctgacgg gccacgatat aatgatcgag gtatttaagt
    15181 ttgacatcca gaatagctgc ctgcatacta tcgagtcgtg aattaacgcc gactaaatcg
    15241 tgatgatacc tcactaccat tccatgattg gcaatagcac gtattttacc ggcaataaaa
    15301 tcatcgttgg taaatatggc accgccatcg ccataacagc ccagattttt ggaagggaaa
    15361 aacgatgtgc atccgatatc acccatacat ccagattgca tctggcggcc atcagaaaac
    15421 gtatatatcg agcctattga ctggcaggca tcttcgatga ttttcagatc aaactcgcgg
    15481 gcaatttcaa taagcgcttc catatctgca ttctgaccga acaagtgaac aggaacaatt
    15541 gctttagtcc tcggtgtgat ggcttttcga acactatcga tactgatatt gaaagtatca
    15601 ggctcaacat caaccacaac tggagttaat ccaagaaggg ctacaacttc ggcagtagct
    15661 ataaatgtga atgttggtgt aataatttcg tcaccgggct gaaatccaag agccatcagt
    15721 gctatttgca gagcatctgt cccattccca accggaatca catgcttcac attcagatat
    15781 ttctccagat tatgctgaaa atcaacaact ttcccacctt taatgaaatt tgtggaatcg
    15841 attacttcct gaatgccggc atccacttca gttttaattt tctcgtactg acttttcagg
    15901 tcaaccattt ggattggttt catcttttta atatttttgt cgtaatgtat tggttattaa
    15961 ttcaatatct agttctcttc cagaaaaaat tcaacctctt cgggaaaaaa acggagattt
    16021 gagatgtaag gtgaattatt aatcacctga agtttttgtt tgcgcttttg atttttcttc
    16081 aactctttgt aatcgaaaac agcctgaata tcattggatt ttattttcga aaagttactt
    16141 aatcccacat tataggttac atcaatcatt acgggaaata tcctgacatt taacttatca
    16201 ggtacattga tgacagtaac aggaatttgc tgtttttttt cggtaaacat ttcggtaaaa
    16261 attccaacat tgatttcttc aaacgaatat tttactccgt tgaccgtttt gagtttcgat
    16321 ttaacaagca gggtatctgc aatttcgtgc agcacaattg gttctgaata aactgccttc
    16381 atggtatcga caatatgttt cggtccgaat acttttactt ttgaaggttc gatacggata
    16441 ctgtcgctgt aaatatactg attggccaat ctaagatccg aattcaatac aattttcaga
    16501 tttttcgaag caagtttatg atagtagatg ggtattgaat ccggttcaat actcagtaca
    16561 gcagtagtgg gcagtacata actgtaaagg cggctctgca actgttcagc ggtaatcagt
    16621 attttaccct gctggaaata aacccgggtt aaatcgagtg taaggggaat tgttttcttt
    16681 ttgttgtatt ccagcagccg aagtccttcg tcacggatta caatggttat atcttttggc
    16741 aattccgata caatttttac gtcttcggga atgccaacat aattaaccgg tatttttatt
    16801 attgattcgc gttcgcgacc tagtgagtga acaaaccaga acattccgga aaggacaaga
    16861 aataccaaga aggtcaaagc atcctttgaa ataagtagtg ctttaacctt caaaaaaaag
    16921 tttttaatcg cttttattcc tgacttaccg gacatagcaa ataaagatta tttcacttca
    16981 ttcggtatat ctgatgctgc agcgaaaaca gaatttttat caactgtgat acgaacgttt
    17041 tcagctattt caactgttac agtcgtttct ttaatgtctt taatcttacc ataaataccg
    17101 cccgaggtaa ctactttgtc acctactttc atggcttcgc gttgtgcttt aatttctttc
    17161 tgacgttttg actgtgggcg aatcatgaag aaatagaaaa ccacaaaaat caataccatc
    17221 atcagaatac ctgaatatcc gcccatacca cctgtaggtt gagccttgcc tgctgcggct
    17281 tgtaataaca cgcttaataa attcataagt tgctgtttat tagattttta gaattatatt
    17341 ttaacgactt taataagttt attgtcggct ttaagctctt taacgatatt atcgagaact
    17401 ccgttgataa atgttgcact tttatcagtg ctgaacgatt ttgagatttc aatatattca
    17461 tttaacgtta cactgaccgg aattgttggg aaagtcaaaa tttctgccaa tgctgcctgc
    17521 ataatgatta tatccataaa agcaatacgg tccagttccc agttttgagt atgctgatca
    17581 atcatccggc ggtaaatagt ctcattctca agcgtatttt ttatcaggcg ggaagcaaac
    17641 tctgcatctt cgtcgtcctt gaacatgggc agaagttctt gattcacatc atttccggca
    17701 tcaaatcgtt taatagtctt gataataaaa ctgattacaa tttccagatc gtcagtccag
    17761 taaatactct gatcttcaat agaatccgaa aattcctcgt tctggagtat gattttttta
    17821 tagatttttc tccagatatc cttatcggca gcatagtcag aagtttcagc ctccatatat
    17881 tccttataaa catcagaggc aataacaagt tcatagatgt tcttaatggc atcctgatta
    17941 ttcgcccagg aagttttatg ttcatacaga aaatcctgta aacatctgtt tttttcaagc
    18001 tgagcaacaa aacgattgtc aacaaatctg gtgttaggat ttaagtcttc ttctgtaggt
    18061 cttaatttat tttttcgtga atcgatttta atagcagcat aatgcgtaat ctccaccgaa
    18121 aattgcaaca agtgaaaata caactcatac gttttttcaa tactgtgaaa taattccctc
    18181 tcagccaaag ttgccgactg tccatcacct ttgtaaaagg aataaagaat ttggataatc
    18241 tttatccgta gtaaaaccct gtttatcata aatttttgcg ctcgcaatga ttaattttat
    18301 gcaaaggtaa aaattttttc agttaatacg gctatgtaca ttcaaaatct taggtattga
    18361 aaataaaata acgtttacct tactttttta aaaaaaaatt aaataaagtt ttcttttcat
    18421 ttgattaatc aaaaaaatat tctgaaattt gagcactgaa agtgactact catgcacttt
    18481 tccgaatatt aacagaaatt attactaatt agcagcaaaa aatcatcaca aagtgagctt
    18541 acagctgtgt taaatgtaca atgttttatg gaaggcgaaa agagaaaaat tgagattgaa
    18601 aaaagagaca gtgaaatcat ttattccaaa gccatcaaag caggaaagag gatttattat
    18661 ctggatgtaa agaaaagtcg taatgaagat ttgtatctgg ccattactga aagcaagaaa
    18721 aaagttacag gctatgatga agaagcacaa gtgacctacg aaaaacacaa aattttcctg
    18781 tataaagaag atttcgataa atttatcgaa ggacttgaag atgtggtcgg tttcataaaa
    18841 aaagaacagg gtgaagcaac tccaagaccc gaatataatc ctgattataa aaaagaagaa
    18901 tataaggaag aagttgaaac agagactgta cttgaagaaa ctgtcggcga gacaaaacgt
    18961 agcttttttg acaaattcaa attctaaaaa cttaagaact cattcgctga gcgtccgacg
    19021 gaaaatccga acggacgctt tttgtacatg ctattttcat caatctatat gttcttaaaa
    19081 tgtatagtcc taaaaaacaa caactttcgc aacaacaata ttttcatttt ttacccgacg
    19141 acaaaataat atgaaatcat tcgccagcga taactattca ggcatccatc ctgaaatatt
    19201 gaatgccatc gtgcaagcta ataatgagca tgccatctcg tatggcgacg atcccgtgac
    19261 cgaagaaacc aaccggcttt ttgaaaaaat atttggtgaa gtgaaagtgc tctatgcctt
    19321 caacggaacc ggagccaatg tggtcgcgct taaatgttgc gctttaccct ttcagtcggt
    19381 aatctgtaca gacacagcac atatccatgt ggatgagtgc ggcgctccga ctcaaagcat
    19441 cggatgttca ctggtagcac tacctacctc tgacgggaag cttactacag aacttatcga
    19501 accacagctg attaatatcg gcaatctgca tcacacacaa ccaaaagtta tttcaatcag
    19561 tcagagtacc gaacttggaa ccgtttactc cacagacgag ctaaaagctt taagtagttt
    19621 tgcccaccaa aacggaatgt acctgcatgt ggatggtgca cgcatatcaa acgccgttgc
    19681 tgcgcttgga tgtacattga aagaggcgac tgttgattgc ggaattgata tcatgagttt
    19741 cggaggaacc aaaaacggat tgatgatagg agaagcagtt ctgattttca ataaggactt
    19801 gtggaaaaat gcgccttacc tgcataaaca atctgcacaa cttttctcca aaaacagatt
    19861 tatggctgct caattcaaag cgctgctcga aacagaatta tggcgaaaaa tggctcttca
    19921 ttccaataga atggcttatt tgctcgaaaa tgaagtcaga aaaatagaag gagttgtgat
    19981 tactcaaaaa gttcaggcca attccctttt tgtaatcata ccttcgtatg cggtggaacc
    20041 acttcgtgaa aaatacaggt tttatgactg gaacactgcg actggtgagc tacgctggat
    20101 gtgctctttc gatacttccg aagaagatat cgccagtttc gctgcaacat tacgaagtct
    20161 gatataaaca aaatcagtct gtcgtgcatt atattacata aaaacagaaa aaaataattg
    20221 tcctatgatt tcagaatcgc ttgaaaaata catttccaat attccgaaaa ccgaacttca
    20281 tctgcatatc gaaggttcgt ttgaaccgga actgatgttt aagattgccc ggcgcaacaa
    20341 cattaatatc aggttcaaga ctgttgaaga acttcggcag gcataccagt ttggcaatct
    20401 gcaggatttt ctggatattt attacgatgg tgcaaatgta ttaattcacg aacaggattt
    20461 ttacgatctg acgatggcat atttccgtaa atgcaaggaa aacaacgtag ttcataccga
    20521 gatttttgca gatccgcaaa cccatctgtc aagaggtgta aaaatggaga cgataatcaa
    20581 tggcataact cgcgcccgtg aagatgccga acgcgaactg ggcatcagtt catatctgat
    20641 tatctgcttt ttgcgtcatc tggatgaaga ttcagcaatc gaaacactcg aagaggctct
    20701 gaattacaag aatgaaatca ttggagtggg cttagactca tcggaagtgg gcaatcaccc
    20761 cgaaaaattc aagcgtgtat ttgcccgggc caaagaagaa gacttccggc tggtggcaca
    20821 tgccggtgaa gagggatctt ctgaatatat ctggggtgca ctcagggaac tgaatgtaag
    20881 ccggatagac catggtatcc ggtgtatgaa tgacgaagga ctgatagact atcttgcaga
    20941 aaatcagata ccacttaccg tgtgtccact ttcaaacctc aagttgcagg ttgtgaaaaa
    21001 tttatcgcat cacccgctca aagaaatgct gaacaacggg cttctggcta tgattaattc
    21061 tgatgatccg gcctattttg gcggatatgt caatgaaaat tttatgcaaa cggctgaagc
    21121 tctcaatttg tcggaagagg acattctcca gttggcaaaa aattcattta aggcatcttt
    21181 cctgcccgag gaagaaaaag aaaaatggct ggtagagctg gataattttc agatttcaaa
    21241 ttgataatta cctgtcagat ttactcaatc atattactta taatacaact actgaaccag
    21301 taactcatca aaatctttct ttcagaaacg gataattctg tattaaccgt gtttttcttt
    21361 tctccacagc aagttgaaaa agacggtatt cttctgtttc cgggtgcatc ggacgatgat
    21421 tgaggtcaga gcgcagctgc tcaagctctt tcctgagatt ctgtgtttgc gaagaaaaat
    21481 aagtatcagt aaaacgctgc cagatataat ccacagccac atccgacgga tgcagcatat
    21541 ccgcagcata aaaacgataa tcccgcattt cgtctatcat tagttcgaaa gccggaaaat
    21601 aatgtacgca atcaaactgt ttttccagaa tatcaaccgc cagatgaagc gtgctcttac
    21661 tcactgtatt ctcatgagcg ccatctttcc agtgccgtat cgggcttact gtaaaaatga
    21721 attgcatctg tggattttga cggtgcagta aaccaagcac gttggtaaac tcactaacaa
    21781 tctcatccac gctgaggcgt cttctgttga aactgctggc aggctgtttg tgacaattgg
    21841 atacaatctt tccattatcg gccagttcaa acacccaggc agtccccagc gttatcatta
    21901 tgacgtttgt ctccttcagg aaatatcgtg cagccagcag gcggctgttg atcttttgta
    21961 aagtttcctt cggattagtc gcagaataag cgctgctgtg tgcaaaactg ttccagagcg
    22021 aacccgactg aaacaaatca tctgcagtaa attctttttc tcccaataaa taacgaattc
    22081 cctgacacac cgacatgggg ttgtacataa ctccgaaagg atttataaaa gtcagaaaat
    22141 aagcatccag tagcttatta ccaatatttt cggcaaagca ggaacccaat gttagtatcc
    22201 ggtcagaaaa ggtgatttcg aacggagaat gaggaatatt tacctgtgtt gaaaacatgt
    22261 tatttttgat ttacaaaaag ttatgaacca cacaaaagta caatatttat cccgatgaaa
    22321 taacaaagcc gcaacggaat gttacggctt tgaattaaaa atatcacgtg tttgtgaata
    22381 attatttttt agcaagcgtg gttttctttg ctgtttgttt tttcacattt gatttagtga
    22441 ctttagttga cttatctttg caacaatcag ctgtacattg ttttgtcatt tgttttcctt
    22501 cgtcacaggt agcttttgca tctttgcagc aagcgccttt ttcatttttt tccattgctt
    22561 ttccgtcctt acaacaagaa gctttaccat tcttacattc cgatttacat tcatgtttgg
    22621 ccattgcctg agcctgatta cattcatgtt ttttcagaga atcggctttt gcacaattgg
    22681 tttttgcctg tttacaaccc tgttgtgttg ctgcattttt acattcatgt tttgcttcgg
    22741 cggtagcatt cttacattcc tgtttagctt tatcgcagca ttcctttttc tgaagttctg
    22801 ccatttttgt ttgagtagta gtttgagctg ttgcaatgct tgctgagatt accaaagcaa
    22861 acataatcat taatacattt ttcattttca tttttcgatt gaatttagtt ttattgatta
    22921 tctgtttaat tcttttttta tgcaaatata tttatttttt ttgttttttc aagttcagca
    22981 ttttttcact ttgctgtgct tttagcagga actgtctcaa ctacttttgc ttcgtaacca
    23041 agtttgttga atgcactaat cagtttgctt tcattggttt tcagcgtatc aaatttgatt
    23101 gttactgttt tatcttccaa ttttacagcc atatctttca ctcctttttc gtaggcaatg
    23161 ttcttttcta tttttttctg acaactttcg caatgcatgg acacatcaaa aactacggtt
    23221 ttctttgaat ttgacttccc agattgtgcg ttcaaatttg tagctaacac aaatatcgcc
    23281 acaaaagtta ttaccagatg ctttgttttc atatttctta gtttttattg tatgattttc
    23341 ttaattcgaa ctttttgcca atgcccaccg gaagcctaaa taaaatgttc ttccgtgcat
    23401 cggaccccag accattgatg catcaaaatc ggtgccaaac ggattttgga tatcaataat
    23461 cggatttttc tgaacaaatc cggtaatatt ttctgcaccg ccgtacactg accaggtttt
    23521 gaaattccgg gttatctgag cattcattat tgtaaatggt ttgaattctc tttcccacag
    23581 tggacgattg gaatcggggt ctggcaaacg gccacctccg ttaaactgtg tggtaaagtc
    23641 atactgccac ttgcgcagcg gtgactgata cgaaagcgtc atcaggcttt tcgtgcggtt
    23701 tgtaagtggt ttctctcgca ggttttcgtt aatagttgtt ttcacgtcat tcatccggtg
    23761 agccagtgta acagtcagac ctttcaccgg ttcaatattc gcctcaatct gaaaactgct
    23821 tgcatatgat ctgccctgca aattgctgaa gttcacagca tgcggatcag tatccatatc
    23881 gattaccacc tgattgttga aatcagtgta gaaccactct cccatcaggc taagttcttt
    23941 gccaaaaacg ggaataaatc cgtgtactgt aaagccataa ttccaggcat tctccatttt
    24001 cagatcagga gcaatattca actttctgtt gcttgccagc agaaaattat tttcagccag
    24061 cacattgggt gaacgatagc ctttaccggc tgatgcacgc aaatgcacat gatccaggat
    24121 attataacgg atgtgaaaac gcggggtaat aaatgttccg taaacagaat tgtggtcggc
    24181 acgaaaaccg gccatagcag tgagcgcttt gcgtgtaaaa gtatattcgg caaaaatacc
    24241 gggcacatat tcggttctac ttaaattctc aggcaatata ttcactgcaa gtttttcgtc
    24301 aatggcatcg gcattgaaac tggctccggt tgaaatctta tgatattcgc caaacgacgc
    24361 atcatatatc agattggcgt acagattatt ctgatgtccg tcgtagtttt tcagtccgta
    24421 tctggcattc tgattttgta tcgttccatt caggattatc ccgaggctcg attgattgtg
    24481 gtgttcgtgt tcctctcctg cttcatgatt atgttttgct tcatcttcac cgaaaaggaa
    24541 tccatttttc acaaagaatt cataacgctc ggtatttact cctattttgt acgaatcatc
    24601 cgtacgtcca ccggttctct tctcggacaa accgcgtacg aatgcctgaa gaatatatcc
    24661 gttatttttg taataccagc gatttgccag gttgtactgc tttaccattg gtaaatccat
    24721 atacccgtcc ttgttttcgt cgagtgtcag aaattcatca gaagcgtgca aaagtacaga
    24781 agttgcgagt ttttcattta gttttactgc tgcgtcagca ttgacttctg ttctgccagt
    24841 gctgcttgta aacaggttga gcgaaacctt atcgctgttc tgtggttttt tgtactccac
    24901 atttatttgt cctgcaatcg cttcgtatcc attttttact gatgcagttc ccttcgatat
    24961 ctgaatgctt tccatccagg gtccggggat atatcccata ccgtatgcag ccgataaacc
    25021 gcgcaaatta ggtatatttt cggtaagcat ctgcacataa gtacctgaaa gtccaagaag
    25081 tttaatctgt tttgctccgg ttgctgcatc gctgtacgac acatcgaccg aagcattggt
    25141 ctcaaaactt tcggataagt tgcagcaggc agctttacgg agttcgtgcg tattgagtgt
    25201 ttccacgttg aatactgcag tacggctttt aatttttcca agtcgctgct tcgttatagt
    25261 gacttccgac aggctgatag catcatcgag tatcacttcc atccgatcct gtttttcaat
    25321 ttttgatata tcaatagaat ctggattata ggccacatta ctgaatatca gaatgttatt
    25381 atcttcagaa gtattcagac ggaaatgtcc atctgcatcc gaaacagttc cttcgtttgt
    25441 tccggcccaa cggatattta cacccggaac agcctgcatg gacttatctt ttacatatcc
    25501 gctaatctga gcgaatatat ttgtcacaaa aaataaatat agtaataaaa tgagctgttt
    25561 tttcattttt aagtttgatt ttttaatgat ttgaaagcac acaaaaggca aatatacatc
    25621 tgtatatctg tcagttataa gagaaaatca aatcagaaaa tcaaatcaga aatactgaat
    25681 tttgagacag aatctgcctt cctttgagtg gttttggaat attgggatga tcggtgaaaa
    25741 gcttgttttt aaatgtttca gcatcatacc cggcaataaa atcaggaaaa agacatatta
    25801 aacaagaatg gagtaatgaa atatcggagg aaaagacatt cgaatcctga atagaaggaa
    25861 catcaactgt aatacgggtt aaagagcacc ccatttcgag attgtgaaaa cacatatttt
    25921 cagattcagg atgtgattga tgttcggttc catcagtgtg ttgatgatga tgaatgcagt
    25981 tgcaactcat ctcagcaacc ttctcaatac ctgcttttgc acaatcctga caacagtatc
    26041 tgataacatt gaatccagtt cctgcaaaaa gaaatattgc agaaagcagt aatgctgata
    26101 tttgatgtga caaatgtatt ttcattttat gacaccaaaa atatatggtt ttattttatc
    26161 ctgaaagtgc aaaactgtta tttaattaga tttaagttaa attatgcatt ttcaaacaca
    26221 ttcaaatcct tgtacatttc acattgcttg tatctgaaac actaattaaa aagattattt
    26281 gaaacataat ttacgaaaaa gtagtaattt tgcgtgatta tacaaattgt gaaaattttt
    26341 tatgaacaaa tatttagttt ttagttttta tcttccactt atattgctga attcctgtgc
    26401 aaaacaggaa atggaaatga agaattacag ttattctcac acatttcatt taacagaaga
    26461 cactacatta ggtggactga gtattagtat tgaagctgaa ttgcctgtta aatatcacaa
    26521 caaggaaata cttacaagca tacagcaaca aattgttgga aaaatgtttg gagagattta
    26581 cacctccctg ccggttgatt cgattattcc caggtatgtc agccggttgt atgacgatta
    26641 taaaatcagc tacggcgacg atctggagaa atacaaaaac ctgaagggtc cgatgctgga
    26701 taatgaaatt caggtggaag cagtttcgat gttcctcgac gataaaatac tgtcatacag
    26761 ctacgaacgt tatgcattta tgggtggcgc tcacggcaac agcaaccgtt atttctataa
    26821 ttttgatctg agtaacggac acatactgaa ggaaagtgat atttttattg acaactataa
    26881 aagtccgtta actgaactga ttaaacaatg tatcgtagaa gacaatgccg agatcgaatc
    26941 agtagccgat ttgaatgaat ttgatttctg ggaagatgag ataaaaccaa acaacaactt
    27001 ttacatatcg gacgaaggca tcgtgtacgt tttcaatcca tacgagattg caccttacag
    27061 taccggtcag actgaagtaa cattacctta caacaggctt aaagtgcttc taaaaccgga
    27121 taatttgatt tcatatcttt acactacaac ctcataaaca aattaaacac catgtcattt
    27181 atcgaactgt tgactaaccg ccggagcatt cgccgatata ctgatcagaa agtcgaaccg
    27241 gaaaaaatac aacaaatact tacagccgca ttgatgtcgc catcatccaa acggagcaat
    27301 ccctgggagt ttgtagtggt tgaaaacagc gatttactcc ttaaactatc agaatgtcgt
    27361 cctaagggtt cacaactgct tgccggatct ccgctgggta tcgttgtgct ggctgataca
    27421 aacaaaactg acgtatggat ggaagatgca tccatagcag ccattctgat acagctggaa
    27481 gctcaggatc tcggattggg gagttgttgg gtgcaggttt ggggacgcga aaaggaagaa
    27541 ggtgtgaccg ctgaagcata tatcagaggg ctcctgaata tccctgataa ttttgcagtt
    27601 ctgtgcatcg tctccatagg ttaccggaat gaagatcgta aaccttatga ttccgggaag
    27661 ctggactata ttaaaatcca tttcgaagaa ttcaataacc acaaccgtta aactcaaaca
    27721 taatccatta tgaaagatag tcgtttagcc tggggaattt ccttactttt atttggtgta
    27781 atgttcctgt tgcgccagtt gggtgttttt tcacctgaaa tagccagtgt agtatttgat
    27841 tttaaaaatt tcccgcttat tataggtgcc atttttctta tctggcacaa aaacaagtct
    27901 gtcggtatgg ttctcgttgt tgtcgggtta ctgctcagat tacaggaaat tatccactgg
    27961 acaaaaaacc tgtcagaatt cgtctggcct ttgttgctta ttgcagcagg gattattctt
    28021 cttttaggag ttagaaaagg gaaataagca catcttgaac gttttattgt aaaaattcgg
    28081 aatgccatga aaattgtttt gttaggagca ggaaatgtgg ctacacattt gggggcgcgt
    28141 ctgttacaga ccggagccga tataaggcag gtttacagcc gcaccgacca gtcggcaaaa
    28201 gaacttggaa gacttctgca tgttcctcat tgttcagatt ccaaccggat tgacaaaact
    28261 gctgatttct atatttacac cataaaggac aacgctctga gcgaagttgc atcaaaaatt
    28321 ctggtacctg atgccattca ccttcacaca gcaggaagtg tctccatgaa cattttcgag
    28381 ggttatgcca ataattacgg agtgctgtat cccctgcaaa cattttcaat tgcaaaaccg
    28441 gtcgatttca gtaagattcc attatttctt gagggaaata atgaatttac aatatctgct
    28501 cttacccagc ttgctgaaaa actatcatcc gtatgttatt tcctgagttc tgaagaccga
    28561 aaaaagatac atctttcagc tgtttttgcc tgcaacttta ccaactatat gtatcaactt
    28621 gcagaagaaa tagtacgtga agccggaata ccctttgaag tgttgctgcc cctaatcaga
    28681 gaaacagccg ataaggtaaa atatatttcg ccaaaggatg cccagaccgg acccgcagta
    28741 cgtaacgatg agcagaccat gctcactcac atcagcctgt tgaagtacaa tgaaaatgca
    28801 cggaaaattt acctgactat cagtgaacaa ataaaacaat tacacaacaa atgaaaaccg
    28861 ctatcattta cacatctgta cacggaacga cagaaaaggt tgtcggttac atggcgaata
    28921 agcttcatgc tgaaaatccg gcagtcatac cactcacaaa agtccggaat tcagatatcg
    28981 aaaactatga cagaataatt ctcggagctt cggtgcatat gggcgtcata cccaaagcca
    29041 tgaaaaggtt ttgcagcgag aatttaagca ccttgctgaa aaaagagttg ggactgtttg
    29101 tatgcggtat ggaagcagat gccacccagc aggaagctga actcgaactc gcctatcacg
    29161 gcgaactcta caaccgggca aaagcaaagg catttgctgg cggcgagttt ttgtttgaaa
    29221 agatgaattt cattcaacgg accatcatta aaaaaataag taaggtaaat accagcacat
    29281 cggatataaa atacgacgtg gttgatcgtt tcattacgca aatgcaaaaa taaatggaag
    29341 catacatccg gattattcgt tggaaaaatc tgatattcat cctgcttata cagtggctga
    29401 tgcagtatgc cgtagttcat ccgatgttgc aaacttacgg tttcgaaatt tataaccgcg
    29461 acattttcaa catactgctt tatgccgcta ccattctgat tactgcaggt gggtatgtca
    29521 tcaacgatta ttttgatgta aagattgacc gcatcaatcg tcccgacaga gtgattgtga
    29581 gcgaaaaaat cacttcaaag gcagcaatga tattatatca gatactgacc ggctcaggcg
    29641 ttatatccgg tctggcactt gccatccttt cccacagttt tacgcttgga tttatctttc
    29701 tgcttacccc tggtttactc tggttttatt cagccagcta caagcgtcag tttattacag
    29761 gcaatctcat tgtgagtttt tccgcttcgc tggtggcact cgttgtgggt gtcctcgaag
    29821 tgtcattact caaaattcag tttggcaatc tgctatccca aaccccgata cccgcgcagg
    29881 tttacggatg gacgggcggg tttgccggtt ttgccttctt ttgcacctgg atacgcgaaa
    29941 tcatcaagga catggaagat attcacgggg accgggaact ggaatgccga accatgccca
    30001 tcaaatgggg aatacaaaag acaaaatact ttctgtacgg attaatagtc ctgacaatag
    30061 cgggactttt ccacgttgac tatcattaca ttcatttcga tggaaatctg actttccgct
    30121 acctgctttt cggggtagtt attccgctgc ttgccctggg ttacctgatt ttcagaagca
    30181 aaactccgga agattatcat caggcatcca cattctcaaa gctaatcatg cttacgggcg
    30241 ttctctacag ccttatattt tattatctga ctgcaaaaac gtatcatata agcatctttg
    30301 acctgttttt tgtaaaataa aatatatgct tgaacatctg aataaatacg atatcatttt
    30361 aggttcccaa tcgccccgca ggcaagaact actggccggt ttgaacatca atttcagggt
    30421 tgaaacaatt tatgtggaag aaaaataccc cgaaacgctt gtaggtgttg atattccgat
    30481 gtatctggca caaaagaaag cctcggttta cacactggaa gataacacga tgctaatcac
    30541 cgccgacacc attgtgtggc acgaaggaac cgtgtatggc aaacccaacg actggacgga
    30601 tgctaaaaaa atgctgcaga cactttccgg taaaatgcat caggtcataa caggggtatg
    30661 tattgccaca aaaagaaaga aaaaagtttt tcatgtaatt tcagatgtat attttgcccg
    30721 ttttaccgac gcggaaattg attattacct gaacaattac gaaccctacg acaaagccgg
    30781 ttcatacgga gtgcaggagt ggattgggta catcggggtg gaaaggatag aaggctccta
    30841 tttcaatgta atggggcttc ccgtgcagcg actttacaat gaactgaaaa actggaaaga
    30901 ttaagcgaca tgaacaaggt ttcggtttgt ttttcacctg ccctgtatcc tttttacgcc
    30961 caaaaagacg aaattgtgat catcatcgac atcttcagag cgactacaag catctgtgct
    31021 gccttcaaca acggagcaag atcagtacgc accgtagcaa caaccgaaga ggcaatcaga
    31081 ttgaaatcgg aaggatatcc ggtagccgcc gaacagaata cacaacgatg tgagtttgct
    31141 gatttcggga attcaccctt tgatttcact gcggagaaag tgaataaccg cgacctggtt
    31201 tttacgacta ctaatggcac acaggcagtt gaagccgcac tcggtgccga agaaatactc
    31261 atcggttcat tctccaacca cggagctctg ctcagatact gcctgaatgc aaaaaaagat
    31321 atgcttttac tttgttcagg ctggaaaaac cgcttttgca ttgaagatac gctgtttgca
    31381 ggcagatttg cagatgagct gatccacgca ggaagttttg tgtccgataa cgatgcggta
    31441 aaagcagcgc gtgatttttg gaaggttgca aaatcagatt ttataaacta tatttgcgat
    31501 actgaacatt atcagcgatt gaagaaaaac gggcttgaaa atgaaattga gtattgcctt
    31561 gccgaagaca caacatctgt tgttcctgtt tatgataaaa cgatcaaagc ttttattaat
    31621 caaagaaatt gaaaaatgaa actaattaac cagaacatga aaattgcaac gtcgcttttt
    31681 acagcattat ttatcttcac taccgggtgt aaaacggaag agaaagtaac tttgccaaca
    31741 gtgaccacat acacagtaac cgatattaca acacaatcgg caacttcagg tggtaacatt
    31801 actgacagta aaggtatgac tgtggccagc agaggagtct gctgggctaa aacagacaat
    31861 cccaccatca acgataacac aactactgat ggaagtggca ccggagatta caccagcacc
    31921 ctgactaatc tgacccctgc cactgagtac tatctgagag cctacgccac caacaaagcc
    31981 ggaacagctt acggcaatac cgttacattt accacccaaa gcaatggaac ttcaactttt
    32041 gccaaaggag cggatatcgg ttggcttcag caaatggaag caaccggata taagttctac
    32101 aacgatgcag gaattgaaac cgactgcatt cagattttga aagatcatgg catcaattcc
    32161 atcaggctgc gaacctgggt aaatccatcg aatgataaag ccagcggaca ttgcagtaag
    32221 gatgaaactg tagcgatggc tgttaggtct aaaaacgcgg gaatgcgcgt gatgattgat
    32281 tttcattaca gcgacagttg ggccgatcca tccaaacagg taaaacccgc agcgtggtct
    32341 ggtcacgatt tcactcaact tcaaaaagat gtctatgatt atactttcga ggtaatgacg
    32401 gctcttaaaa acgccggagt aactcccgaa tgggtgcagg tcggaaacga aatcactcca
    32461 ggaatgctgc tgcccgacgg aggttccgac aatgcacatt ttcctcagct tgttaaactc
    32521 atcaatcagg gttacgatgc tgtaaaagct gtctgtccga cttcaaaagt cattcttcac
    32581 attgatcagg gtaacaacaa cgcccgtttt cgctggtggt ttgataatgc aaaaacctat
    32641 ggtgcaaaat acgatattat cggtttaagt tattacccct attggctatc agattctcat
    32701 cccgactata ccttgtcgat tgacgattta ggaaaaaaca tgaatgatat ggttacacgt
    32761 tacggcaagg aagtgatggt ggtggaagtt ggtggagaag acggacagga tccacaactt
    32821 agcgcggcac agaacgcggc aaaaatccag aacacctacg atatgctagt ggctgtgctg
    32881 caaaaaaccc gggcagtacc caacggaaaa ggacttggag tattttactg ggaaccgcag
    32941 ggagcacgtt cgtggagtca ttatgctctc agtgcctggg gaaatgacgg taagcctacc
    33001 cgtgcgttgg atgctttttt gaaatagaaa gaatgtgaaa aataaaacta ttgaaagttt
    33061 aaaagtgttt tatattggta taatattttc aactattatt aaggacaccg aacataaaat
    33121 taaattaaaa cgaattatta actcattaaa taaggagaaa aaattatgtt aaatccaaag
    33181 atttcaaaat tactcaacga tcagattaac aaagagtttt actcatctta tctgtatctg
    33241 gacatgtcta attattatta cgacaataac ctgaaaggtt ttggcaactg gtttgccata
    33301 cagactcagg aagaatacgc gcatgccatg ctatttctga aatacatgca aaacaacggc
    33361 gaaaaaatta cgttggaagc cattgccgct ccgggtttga aatatgcaaa tctgaaagca
    33421 ccgcttgaag aagctcttaa acacgagtac tttattactt catcgatcaa cgatatatat
    33481 gcagctgctt atgatgttaa ggatttccgc accatgcagt ttctcgactg gtttgtaaaa
    33541 gaacagggtg aagaagaaca gaacaccgat gatctctgca aacgttttga tttatttggc
    33601 agcgacccga aaagcctcta tctgctcgac aatgaactat caacaagggt atttaatcca
    33661 ccttcgctgg ttatttaatt atagtttcag acattaccgc aatattaaat tcagagcggg
    33721 catccagatt aaaggatgcc cgctctgtta tttgtaaatt attcgtttat tgagtttata
    33781 ataaaaaaac tgcttattga gtttattatt aattacatcg ttttaaataa tgatgcatta
    33841 cgaatgtaac aaaatattcc gaaaatccaa agttttaaaa ttaagaaacg aacgaatcca
    33901 aaaatcagtg atttacgaca aatttcttca caaatatctc gctgcctgct gacaacttca
    33961 gtatgtaaat acctgatgga aaatccgaca catcaagact aacattgtca tattccgtca
    34021 atcgttggct aaaatgtata tttccgctta catccaacac cgatatttcc gatttttctg
    34081 cacttatttt ttcattgaat tttatattca acactttttc tgctggtaca ggaaaaattg
    34141 aaattgcctg ccggcttacc gatttcaaat cttcaactcc tgaaagaatt ccggctattt
    34201 ctgctattcc ttcgggtgtc aaggcataat tatatactct gaaatcatcg atattaccat
    34261 tgaacaacgg ataagagaat tgactcctgc caatataatt cagtgctggt ttaaaatcta
    34321 atggttttat agtcattgta tttgactcgc ttacctttat accattcaca tataagcgtg
    34381 cagtgtcggt tgccaaagtt acggctacat gaatccattt gcttcctgca agcgatgttg
    34441 cgttcagctg ctgttcgtca cctccgtttt taataccaaa acgcaactgt ccgtttccgt
    34501 tattcggggt aagatacata tattcattct ggctgttgcc aaagtcaaaa atccgttgcg
    34561 ctacagtgcc tcctctgcag taaacccagg ctgcaatagt aatttcctgc tgattggcga
    34621 tattgggcgg tagttgtaaa aaggcatttg taccgttcag tgaaattgca tttgcacccg
    34681 ttttaccggt tgtgtaggag attgagcctg atgatgcagc atgattgagg tttacggtac
    34741 tgtccgaaat attgttttca aattgcagac tagctaccag gtcgtttgca ccggttgtaa
    34801 tggctgatgc ttcgttggag taaactgatc ggttcagcga attatccaca gcccgaacag
    34861 cgtagaaata ttgtccgttt tcagtagttg tattatcaac gaaagatgta ttctttatgt
    34921 tacgggctat cgtttcaaac tttccttcgg gtgagtcttt gcggtaaata ttgtatccgg
    34981 caacatcggt atcagcgctt gctgtccatt ccagttttac cgactctgcg ttgggtgtgg
    35041 cagtcagact tgctggtgcc gccggtgaga caaactctac cggagcaccg acaggaataa
    35101 acctccactg ctgattgcgg tctccgtctt tttcccactg atacacatta gcgccgttag
    35161 tcatgctggc atttgcaact tcaatgcatt tagcgctgtg tctgcttcga atataaaacc
    35221 agccatcttc agcatattca agataccatt gctgattagc tccatttgca acatcccact
    35281 ggacgatatt tccggtgtta tcgagcgacc agttataaat atcgagcgat ttactgcttt
    35341 ttacagaagt aatggtaaaa tagctgaaat cacctccaat ccgtgaatca accggtgtta
    35401 cgttccattg ttggtatgtt gcgtccggaa taaaactatt ctgccacaca ttagctcccg
    35461 atgaagtact gccaaaggtt acctccatca ctttaccgct attcctgttt accaaaacat
    35521 atctcccgtt aattaccggc tggatatcat cgccccaggt tatattcatc actctttcgg
    35581 catttgtctg tcctttctga tatcctgtgc ctccgggcaa aaccattgta tattcacgct
    35641 gaggaccata tccgtcaaag taaacatcct tagttttaga gaagaaacga tatgttgttg
    35701 tcgccgcctg acgctctgac gttcctccaa atgcctgaat ttttccatcg ggtgagcgat
    35761 ataccgatgc cgctgtccag ttgggacgat gctcggcgta acccaatctg acgccgtcac
    35821 ttgctttgac aaattctccc cgggcatatt cggctgttcc ccaccagatg ccagtcttca
    35881 taccatattc cacgcctacc attgcctcca tcacattgtg cagttcgtca ttcgttccgt
    35941 gatctccatt tgccgccaca cttttatgga agttggcata attgtcaaaa cttcctgcca
    36001 actgatgagt gttgccttcc tccagacggt ctttcagata gttataccat gacaaagcct
    36061 cgtcgcaatt caacgtgtta ccgcccgaaa tccggatatt gtcaaaccgt ggatttttcc
    36121 tcaactcacc tgcaatattc ttgaaatcag acattccgtt agactttgac ccggagtatt
    36181 gcccccatcc aaagtcaggc tcgttaaacg gagctaccga gacaacggtt cgtccccttt
    36241 cctgaacacg cctggtggtt acatcaatca attgtgccca gttagccgca ttcccaatat
    36301 accatgaatc aactctggga tggtcacaat ttaatgctac tttcgtttct gttcctgtca
    36361 aatctatcag atttagacgg atgtttaagc tgtcaatctg agcagtttgc aaatcgccgt
    36421 ttaccaacgg ataagtaggc tgaaaggagg ctctgacaac atccacctta cggggactca
    36481 taaacgcgat gccacgccgg atattcgatt cgctgagcca tgccagatcc aagccccaga
    36541 tgatgggttt actcactccg ggatccgcaa cattaaaaat aaccgtacga tccgaagccg
    36601 gttgaacaga gaaataactg ctggcacttg tagtctgact aaaaaggggg gtggcattaa
    36661 ttcctgcaat tgtaacaaat gccagtaaaa atattttatt gctcatatta ctcattttat
    36721 ttacaaatgt agctcctttt tatcattcca aagagtattt attaatcaaa aaggtttgca
    36781 aatcattcag ttgttcattc tagttactaa ttcaaaggtt tgcaaattgt ttttatacct
    36841 cttcaattcc ggtaaacaaa aaacattcct gattgttaaa cattcaataa acaataagaa
    36901 aagatgaatt acaatacaca aaaaatgcct gttttattcg tcggtcatgg caatccaatg
    36961 aatgccattg aagacaattc atttacacag acatggcatc agataggtaa aggaataccc
    37021 cgtccgacca caatactcag catttcggca cactgggaaa ccgaggggac ttactttacc
    37081 gcgatgcaaa ctccgcgttc cgttcacgat ttcggaggtt ttccgcgccc attgtacaat
    37141 gtggtatatc ctgctcccgg taatccggaa ttagccgaac aaataaaaag tgtaattaaa
    37201 ggacaggtta ttgaacttga tgaactggaa tgggggctgg atcacggaac gtggtcggtg
    37261 ttggtacaca tgtttcctca agccgacata cctgtaatac aaatgagcct caaccgaaat
    37321 atgacacccc ggcaacacta cgaactggca caggagatga gttatctgcg tgaacaaggt
    37381 gtactgataa tgggcagcgg aaatatcgtc cacaacctgc gcgaagttga ctggcacaac
    37441 ccctactccg cttacgactg ggcgctggat gttaatgata aaattaaaaa ccaactccgt
    37501 aatcacgatc atgatagtct tattgatttc cggaaacaag ggcttgagtt caggatggca
    37561 attcccacag ccgaacatta tttaccattg ctttatctta taggtttaca atccgatagt
    37621 gatgaaatat cgttcttcaa cgataaaatc agcatgggat cactttccat gaccggagtg
    37681 atgctcaaat gacgtaatca ccagttcctt aaagccatta gttgacaaca aaaaaagatt
    37741 gataaatatc agttcaaatt gggcattatc gaaaattgtt aatatatttg tcgcgacatt
    37801 aacaatattg ttcatcatta tttcctgttt tgaatttatc attcatcatt catcaaaagt
    37861 tatatcaaca tggaaaattt taccgacctg tcgttcaatt tctggtggct tgttcctttt
    37921 atcgtaattc tggccgtttg ggatacggta tggaagttgc ttggattatg gtaatcagcc
    37981 cgcaataatg atctgatctg gtttatactg atagcaatta tcaatacttt gggcatattg
    38041 ccaattattt atctgctgct gaaaaaaaat aaaacggaac aaacggaata atcctgatgt
    38101 tcattatctg aaaaaccaaa cctattattt caccggatga ctgtcgttat catgtaatcc
    38161 ggtggatttt ttttgcaatc ttcattaaca acataataca aataccattt ttattttact
    38221 attctagttc cttttaccat gatcacaccc atttccactc cagcctccac tgaacgtatt
    38281 caggtagttg acgcgctccg aggttttgcc atcgcagcca tacttcttct tcacaatctc
    38341 gaacattttg atttctggta tactccacag ggttttcctt cctggctggt aaatttggat
    38401 aaaggcatct gggataccat gttctttctg ttctccggca aggcatacgg tattttcgcg
    38461 ttgcttttcg gatttacgtt ctacatcatg cagcacaatc aggcaaaaaa agggaaagat
    38521 ttccgtctga ggtttgcctg gcgattgctt ttgctgcttg gtttcggtgt catcaattct
    38581 gcattctatc agggcgacat actcacaatt tatgctctgc tgggcatcac actcataccg
    38641 gtggccaaac tcagcaacaa agcggttctc atcatcgcct gcctgcttat ctttcaacca
    38701 tgggaatggg gaaaattcct gattgccctc acccaccctg cgcaggattt agccgatccg
    38761 gcatcgtggg cttacttcgg aaaaatgagt gaatatatta ccggaaattc atttatcaaa
    38821 accattgccg ggaacctgac caacggtaaa gcaggtgttt atctttggac ctgggaagca
    38881 ggacgtgtat tccagacacc cggattgttt atgatcggga tgttgctggg acgtacaggt
    38941 aaatttatat tttctggcga aacaaaacgt ttctggacaa aaattctgat aatttcctct
    39001 gtggcatttg ttcctctcta cctgttaggt atcggattaa ctcccgggaa tataccgaat
    39061 gaagctttat taagaccatt atctctgata attaaatcct ggtcaaacat cgctttcatg
    39121 tttgtactga ttgccggatt cgtgctgttg tttcagaaaa gtacgattca acgcgtgctg
    39181 aatgtgtttt cagttttagg taaaatgagt ttgtccaact acatcattca gtccattctg
    39241 ggagccacca tttactacgg tttcggactg ggtttgtacc aatataccgg agcaagtatg
    39301 tgtctgatta tcgggttggc actcatgatc ctgcaatggc atttctgcaa atactggatg
    39361 cgttcgcacc gctacggacc gctagaaggc atctggcata agcttacctg gattaatagt
    39421 gaaaataaca agtgatgacg aactgccagt ttatttacct gttttccgga attcgacttt
    39481 cacaagggaa gtaaataccc caataaaaca taacatcgta aatagtgcaa agactgtttt
    39541 tgtactgagt atcaattggg ggatattcga agcaataatt tgcgcgttgc ccaaaaacaa
    39601 atgaagtgtc agtgaagcaa tagccatact gaacatcatg ccggtgttgc gcatcgttcc
    39661 caatatagcc gaagctgttc cgtatatttt tttatcgacc gaacccatca ccacattggt
    39721 gttgggcgac gaaaataatc cgaaacctgc gccaaggact attaaaccga taataatata
    39781 aatgatcgtg gtctctgagc taataaagct tagtattaac aacccaatgg cacttattcc
    39841 cataccaagc gcggctaagt ttcggggatt cattttatcg gacagccgtc ccgaaaaggt
    39901 tgaaacaatg gccatcaata cgggttgagc tacgagaacc aatccggctt cgcgtgcttc
    39961 catacctttg acgtattgaa gataaaggct gagcataaac gatatggaaa aagtcgccgc
    40021 gtaattgatt attgctgata aattggaaag cgcgaatacc cggttctcgg taaataaacg
    40081 gatatttaat acggggaatt gagtttttaa ctccagtttt gcaaagaaaa acaatccggc
    40141 aatcccggct atggtcagaa caattgccag ctgatgaggc aactttgaca gtccgtacat
    40201 cagcatcgag actgaaatca tgtataaaat agtacctttg tagtcgaatt tttcttttat
    40261 tgtttcggac aattcccggt tcagtttcca aataatcagt atggctatca gtgtacttac
    40321 cacggcattg ataagaaata tactccgcca gctgaacgcg tcggttatta accccccgat
    40381 catgggtgcc agtgatgacc ctacgtaaac cgcagagaca ttcaagccga tcatttttcc
    40441 tcttttttcg ggtggatatg ctgaaattac aatggccatc aatgtactta tcatcatagc
    40501 tgaagccata ccttgcataa atcgaatgat aataaatgtg gttgaattgg gaacaaaagc
    40561 acacaacaag gtggtaatgg caaaaaaaac tgttccatat aaaaacattt tggttctgcc
    40621 ccaggtgtca ccaagtttac caaagggcac cagaaatatg atcgatgcca gcaagtacga
    40681 catggaaacc cagctcaatc caatagcagt cattgaaaag tcagtcccta tcttcttgag
    40741 ggcaacatta actgctgctc ccatgaacgg attgaaaaat tgagttgcca tggttgcaac
    40801 cagcaagagg gttttattaa tgtttttatc tgtaagcggt gttgatttca tgccgttgta
    40861 ctattctgtg atttttatga tgtataaatt acaaattgat aatgtatgca ttatcattat
    40921 tttcagtaat aagtaacctg agtttttctc tgtcgtcagc acttaaatat gaagtcattc
    40981 agtattttct ttcttcatga aagcagcata caaaaataat caaatgaaaa tatacttgct
    41041 acttcttgat aaccgaaaag aagaatttct tcaggtttac atacaaacca tttaccaaca
    41101 atagcatttc ctctgcagta tctctgtttt ttcttctagc ggtcagcggt atagttctga
    41161 tatcaaatca tttagttcta aaataccatt tttttcgatg tggagattat cagagttcat
    41221 gagcctgtga tattacgaca ttcagacttt atcgaaccaa atgagcctga aatctgtttc
    41281 ttcttataga aataaatact ttaactttaa tcctgagttg ttatgtgttt tacagttgca
    41341 ataatgaaaa acggagttct gatgactgcc gaacagtatt acaacagtct gcctgccgtc
    41401 atccgcaaaa agaagagaaa ccctttgcag acagaaatac ccaacttcta tctggtcagc
    41461 ggatttacac atcccatgct tccggtgatt accgacgatg agatgaccct gaaacaatgg
    41521 ggactgatac ccgaatggac tacagaccga catcaggcgg atgaattgca aaacatgacg
    41581 ttgaatgctg taggtgaaac tgtttttgag aaaccgtcgt tccgaagaaa catactcagc
    41641 aggcgctgtc tgctgccggt gagcggattc tacgaatggc gcgaattcag aaatacaaaa
    41701 tacccctact tcgtacatcc taaggatgca tccggttttc tactgggctc cctgtacgac
    41761 cgttggactg atcctttgac cggcgagcat cacgatacct ttagtattat ctccacaccg
    41821 gccaatccac ttatggaagt gattcacaat gtaaaaaaac gaatgcctct gatactcgat
    41881 gatgacgctg ccgaccgctg gctcaatccc gccacatcgc ccgatgaact gaaaacactc
    41941 atcaaacctt atgacgagaa caagatggac gcctatacca tcagcaaagc ggcaggcaat
    42001 ccgaatttcg acaggaatat tcctgaaatt ctaaacccgg ttaagtattc cgagttagac
    42061 actccgacac tgttctgaaa agatagatta ttttcgggga attcaggtca ttaaacacat
    42121 tgataatcaa tatattgaat acttcattcc ctgacaacac aaatactccg gcagagtgat
    42181 gagaatgaat tctaaaataa tacatattcg agaacaaaac tgaatagatc aacgttttaa
    42241 tgatataaaa aaaacagagc cttaactggc atgatcaagg tcgcttgctg acactattcc
    42301 tgagcagaat ttcgcttagg ctttaattca atgatttctg attttactta acccaattta
    42361 atcttcaata ttatgccctc aaagaaacta attgattttc tcaatgaaaa tcagattaag
    42421 tttgtcttaa ttcatcattc aagggcgtat acagccaacg aggtagctca ttctgcccat
    42481 attcacggtc aggaacttgc caaaaccgta atattgaaaa tagacggaaa gctggctatg
    42541 gctgttcgtc ccgcatcggg gcttgtaaat acacggctgc tgaaagaaga gctgggagca
    42601 agcatggtgg aagttgccgc tgaacatgaa tttgccaacg ttttccccga atgtgaagtt
    42661 ggtgctgaac ctcctttcgg aaatctctat ggaatgaatg tttatgtatc cgattcactt
    42721 gcgcaagaac ctgaaattgc attcaatgcc ggaacacata ccgaactgct tcgtatgaaa
    42781 tacgaagact tcgagcgact ggtaaatccc gaagtactac gtttttaact gacacacgac
    42841 cataaaacct tacttcctgg tgaaaaattg tctcaagttt atgaaaaaaa ccccggctgg
    42901 ataacataat ccgaccgggg ttgtattttt atttcctgac aaaatgcatt actaattctt
    42961 cacacacctt accgaatgcg cgttggtatc aaaatcctga ttctgcgaca catcagatgc
    43021 atcgtaatgc agacagtaaa cataataggt atagttattc aaggctgtag ccgaccacca
    43081 cgaagctatt tcttcaatac cccaaaactt atgtgcatcg gcatcatatt caccaccggg
    43141 caaagctgag aaaccgctct tgttattggg ctcggattcc agctttttcc atgtaatggt
    43201 tttgagctta tcgcctgcaa aacggtgatc gccctgagat ttaatcagta ttttccactc
    43261 ttcgtcactc ggaatgtgcc atccatcggg acaagcctta atggctgcgg cataatcgta
    43321 aaggtatccg tatacagcta cattctttgg attattgtca tacgcccagc aacccgcatc
    43381 ggttttgtat gcgaggtttt caagcatcca ggtttgcgta ccgatagtgg tagttttata
    43441 aattttcccg tccctgttat ccgtaaagat acccttttcc tgcgctttca ctgtcagtgc
    43501 ctgcaggtac atgataggta tcagtattaa gattaggttt cgcatggtat ttatttcgga
    43561 gtatcagatt aattgagatt aaacactgac ttcattcgtt cagcaaacgt tttgtttgct
    43621 ttttcccgga gtcttcttct gcctccatga ttatcctttt tgccttgctg agacgaatca
    43681 gccggttttt ttcggtctgt ttgtattttg tccatttctc gagtttgtaa tgcaacggta
    43741 tcatcagaga agctacagat acgaaaatta acagcatcag aacgggagta tgatttgtca
    43801 ggttggaaat aatggggtgt agcagctgat cattgtattc aaatgtaata agcagcagcg
    43861 caatgcccag tggccttaca gaacactggc tcacaataaa actccggttt agtaacagaa
    43921 acgcgattat aaacacaata atcactatgg ccagaacgct gagttgaagt cgttcgctat
    43981 gtacttttgt ctgatggatt ttctccattt ccagctacaa tagaataata agtaataact
    44041 ttttcatcat gaatgaatat caggattttc agcctgacgt cagttatatg tctttctcat
    44101 aatgaaagag gaagacacag tagccggtct tcctcttgtt gtgtgattat ctatttaatc
    44161 tcgatcatac gtgaagttct gggtttggtt tcttcttttt taggtaccaa aactgtcagt
    44221 attcctttgt cgtagctcgc cgtgactcta tctgcatcgg cgtcgtcggg cagggtaaac
    44281 gaacgtataa aagcctgata gctgaattct cgtttggtgt aacgttcacc atgcttaccc
    44341 ttttcatctt tggtttcact ttcttcctgt ttttcggaag aaacggtcag cacatcgttg
    44401 ttgacttcaa ttttgaagtc gtttttgtca aaacccggaa ccgcaacatc aatcagaaac
    44461 tcttctttgt tttcacggat attcacagat ggcaatgtcg tgtttgtttt tgagaaattc
    44521 ctgtttgacc agtccaataa atcgttatca aaaaagcggt ccaggggtga ttgtgtaaat
    44581 cgagccagtg acataatttg atcctcctta tttacttgtt aaacttttta atttaaactc
    44641 actcgttttg ctatctcagg ttttgcttat ttgtattatt aaaacaattt atattctgtt
    44701 ctgttgatag tgtcaatctg ttttttcaat caacactata aatacagtca ctttttggga
    44761 atgtttttac gcagttttct aataattgct cgattatggt gttcttcaat aataattctt
    44821 agatcagggt gcacatttct gactgtcagt ttactttcag tcaggagatg atgatttttc
    44881 tgttcgtgtt ccttatcgct aaaaaacact ttaatgtcag catccttctg gtgttccaca
    44941 attgatactt tcttagtttt catgattttt cccatgtact ttatgaccgg attttttggt
    45001 tgtttccttt tcggttatgg ggtcgttatg cagagaagta atatggtttt cggtcttttc
    45061 aagatatacc ccgatgcacc gatccttgaa gtttacacac acctgtgtca ccttcaaaaa
    45121 ttcattttta gcctccgaat aaaggaacaa accaatctgt aaagctgaaa gtccgtaggt
    45181 gtcgataaac tgttgaaccg ttttacattc ttcagtcaat gttgccctgg taattttgtc
    45241 gtgtacataa ataccgaagc cttcttttgt aatataaccc gttattccgt tcatgaattt
    45301 atttttcagt acagaaaaaa ttaaacttag taatgattac tttgaacatc agtattcttc
    45361 aggattcaat aattccttgg taaaacacag gaagtcttta taatgtaata tcagaacgca
    45421 atcgtataaa ttcaagaatg agctccagac ttttattaat tccaagtttt ccagtgttca
    45481 gagtcaggtc aaaatttttt gaattgttcc agtccattcc tgtgaatgta ctgatataaa
    45541 gtgcacgttc tttgtctgtc tgctttatca tattgttggc ttcctcttca gaaatgttgt
    45601 ataatccctg aagacggcgg tttctgaatt cgatatcggc atgcagacaa atatctatcc
    45661 tgtttggaaa atcgcgcagt acataaaatc cgcctcggcc tataatgaca gctgagtgct
    45721 cggctgctat ttttcggatg atttgagatt gttcattgaa caattcctgc gatgtaggta
    45781 caaaaatccg tcgtgtgggg acatatacgt tgggtaccgc tgtaccgtaa aaattccaga
    45841 gtgacttcca caagggttct acacgttcct cctgcgaagc tacgtccaca atattagccg
    45901 aaaaatattc tgcagctttc gctacaattt cataatcggc ataatacata tttagctttt
    45961 ttgctatttg ctggccaata taagctcccc cactgccaaa ttcgcggctt attgtgacaa
    46021 ttaacggtgc attctgattc attttttttc ctccttataa tgtttattaa tcaaaatact
    46081 gtaattttgt gcgcacaacc ttaaacagac ctttttttga agtttcttac gcaaacctgc
    46141 aattatagtt cagttcgaaa atacagtcag gatgtatata attaaatata taccttcctg
    46201 atattaaaac agtaacagag agttaaacgt tcgtgctgca aggtaaaaaa aacctatatt
    46261 tccttaacat ataaatgatt gtgtcaaaca atttgtttat ttgcacatag gtttaacccc
    46321 gcaattaagg tcctgttata tcaattgttt aacaggtact ctatcatgag tatccattct
    46381 ccgaaaaaaa atcacccccg gctttttgat tcccgtttct ttcaaaatac acttttcagt
    46441 gaaatttttc tgaaaaaaca tatttgaaat acaacttcgt ttttcaattt taataaaata
    46501 aaatgattat ccgttgtatt acctaaattg atttttatat gtaaccgagg c
//
LOCUS       DAOPYB010000006        44191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_713_length_44191_cov_10.762529, whole genome shotgun sequence.
ACCESSION   DAOPYB010000006 DAOPYB010000000
VERSION     DAOPYB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 44191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44191
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_713_length_44191_cov_10.762529"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..534)
                     /locus_tag="PKH58_00865"
     CDS             complement(31..534)
                     /locus_tag="PKH58_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNX87606.1"
                     /translation="MNITPDTKIADLLKEFPQLEATIIRYSPAFSALKNPVLRRTVAK
                     VTSLQQAAKVGNVSIVEMVDTLRAEVGQSPLHDEYAQIENSDINAEIPSYRVTHHYDA
                     RPVIDAGDHPKDAIFELAKTMKSGDCLEILTPFPPVPIADFLTNKGYKVTISTTQNGV
                     VKTYVKI"
     gene            complement(616..1857)
                     /locus_tag="PKH58_00870"
     CDS             complement(616..1857)
                     /locus_tag="PKH58_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HNX87607.1"
                     /translation="MSEYIDNSTKRKDLLKHLLIQLHEGAPASVLRGRLIEVLSSIPY
                     NEVVEVEEELINSGTLTSEEILDFCDLHTSVLDGSIDLEGAKTIPPGHPVDTFKKENI
                     AIRNEIDKAHHLFEDLKSVTKENINEYVIKIRTVFNALSDIDKHYKRKEYLLFPYLEK
                     HEVTGPPKVMWGKHDEIRGLMKDIYEALETPEIDVEELKPIIEMVIKPVLELIEGMIM
                     KEEEILLPMTMDTLTSEEWYKIYKETPEFGFCLVDPEAEWKPEDVAETQPEFIHTDAI
                     RLSSGAFSMEELEALFIHLPIDITFVDKNDKVQFFSHSPNRVFERNRSILGRDVRMCH
                     PPGSVHIVEQILTDFKSGKENKAAFWMSDFKGRFIYIEYTAVRSPEGEYFGVVEITQD
                     ITELRKLEGDQRLLSYSKIEK"
     gene            complement(1882..2208)
                     /locus_tag="PKH58_00875"
     CDS             complement(1882..2208)
                     /locus_tag="PKH58_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX87608.1"
                     /translation="MNTLFNNGEVFPLETSIEYTGGGVVSKQITKSPAGNVTLFSFDK
                     GQGLTEHTAAYDAIVEILDGEAEIRIDGEPKIVKKGECIIMPANHPHALNAVQPFKML
                     LIMIKG"
     gene            complement(2668..2940)
                     /locus_tag="PKH58_00880"
     CDS             complement(2668..2940)
                     /locus_tag="PKH58_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX87609.1"
                     /translation="MENKPASIRELRQIPGVGPSIANDLYRIGIRKIDDLKFKNPEDL
                     YDWSNQMAGCVQDRCLLYVFRCAVYFAETDPENREPEKLKWWNWKI"
     gene            complement(3037..4044)
                     /locus_tag="PKH58_00885"
     CDS             complement(3037..4044)
                     /locus_tag="PKH58_00885"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNX87610.1"
                     /translation="MKVAIVGASGAVGQEFLRVLDERNFPLTELVLFGSSRSAGKEYE
                     FKGKKLIVKELVHGDDFKGIDIAFTSAGAGISKEFAADITKFGAVMIDNSSAFRMEND
                     IPLVVPEVNPADAKNRPRGIIANPNCTTIQMVVALKALEDVSHIKRVHVSSYQAASGA
                     GAAAMDELELQYRQIAKGEEPTVSKFAYQLAYNVIPHIDVFTENGYTKEEMKMYNETR
                     KIMHSDVEVSATCVRVPTLRAHSESVWAETERPISVEEAREAFANAQGVVLMDNPAEK
                     IYPMPLFLSGKDPVHVGRVRKDLSNPNGITFWCVSDQIKKGAALNAVQIAEYLIAEKD
                     I"
     gene            4165..5955
                     /locus_tag="PKH58_00890"
     CDS             4165..5955
                     /locus_tag="PKH58_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX87611.1"
                     /translation="MKKSLIILIILLPFFAFSQINETFSDGNFTENPVWTGTVQNFRV
                     NSENQLQSQAAATSKSYLSTQSEAFEDATWEFWVKIAYTTSSSNYASVYLISDRADVT
                     TECYGYYVQIGNTNDEISLYRQEGTTKTKIIDGTDKLIDANPVEVRVKVTRDKSGKFT
                     LYRKLSSDNDYVTEGEEVVDNKIQGSKYFSLLYSNSSTTGNAYFFDDISVAGEKLIDV
                     TPPVWTSLSLIQPDKLILTFSEPIDFTSTEFQVDNGIGSPSKQIVSADKTTVELTFTN
                     NFEKGKIYVVEALNVKDISGNALENPQKQIGLVEPIEVGDLIINEILFEPAEDVPEYF
                     EIFNNSGKVLDVSKVFFGTRKTDGSFIPANNFPPKTLLLPLQYLALTPDANAIRNRYQ
                     APDTARILSSSKWSALNNSSAALLITNSTGDTIYDEVKYDVKWHHPMVRITTGVSLER
                     INPSLPAQNAGNWHSAASEVQYGTPGYKNSQYRDISSTKTTEKWVRTDPESFTPDNDG
                     MDDVCFIRYITEGNGFTANAMILNSVGVKVRQVAANQLLSTDGFFTWDGRTDNGKSVN
                     PGIYIIYFELINANSGVKKTEKIPVVVSAR"
     gene            6043..6630
                     /locus_tag="PKH58_00895"
     CDS             6043..6630
                     /locus_tag="PKH58_00895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX87612.1"
                     /translation="MDKVSYALGLSLGNNLLSSGITALNVEKFSKGVQDVLEQNQPEI
                     SYQEAQNVINEYFQNLQAKLSEKTIAEGKAFLEENGKRSEVITLKSGLQYEIIKEGNG
                     SVPTANNTVNVHYHGTLIDGTVFDSSVRRGEPATFGVTQVIAGWVEALQLMPVGSKWK
                     LYIPSNLAYGAQGAGQHIGPHTTLIFEVELLGIEK"
     gene            6668..7468
                     /locus_tag="PKH58_00900"
     CDS             6668..7468
                     /locus_tag="PKH58_00900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX87613.1"
                     /translation="MKKISILPLAALLLVGCSSVKKVETPVVPVAPVNILTNDVDSMS
                     YALGVNVGEDFAKNIKTIPGGKSNIDLLIKGFASAMKGDSTLLSKDVAQTYFRDYLTK
                     AQNKQNDLVKAEGEKFLADNKLKEGVQVTSSGLQYIILKTAEGAKPQSTDKVKVHYTG
                     TLMDGKVFDSSVERGEPVEFQLNQVIPGWTEGVQLMPVGSKYKFFIPYNLAYGEQGVS
                     QAGIPPFSPLTFEVELLDIIKEQPAPVVEEVKPAAETKTPAKKSTKKK"
     gene            7491..8000
                     /locus_tag="PKH58_00905"
     CDS             7491..8000
                     /locus_tag="PKH58_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HNX87614.1"
                     /translation="MKKQNFLTMALIIAILGLASCGSNYKAKKANLSTQEDSLNYALG
                     LAISEELRMNALQNDSSEKTIAALVEKIEKVYSDENTDQVYKTGFQIGNMMKEQNKVG
                     LIGDSTLKFNQDLFKKGLVKGINGDTTGMTGPQAMVYFQNTMQKIQNEKMMKSAPASA
                     PAPADTIKK"
     gene            8030..8899
                     /locus_tag="PKH58_00910"
     CDS             8030..8899
                     /locus_tag="PKH58_00910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX87615.1"
                     /translation="MKKQNIFFAVAIVAITLLTACNGGKSGSTKDPKLASQIDSLNYT
                     YGLAFGNDLKTNMFNKDSVEQKTKTFMKGLAEGAKGEPDAKPGLSAAANQYGEWLKQQ
                     KTAGFLGDSTLKVNFNLFKQGLINGLNKSKIQMTPQEANEYINKVMNARQEKIMMKQF
                     GKNVEAQDKFLAENGKKPGVVTTASGLQYEVIKKGTGPMPTATDQVKVHYVGTLLDGT
                     EFDSSIKRNEPAMFAVNQVIPGWTEGLQLMPVGSKYKFYVPQNLAYGSKGARGIEPLS
                     MLIFEVELLSIEK"
     gene            9085..9540
                     /locus_tag="PKH58_00915"
     CDS             9085..9540
                     /locus_tag="PKH58_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNX87616.1"
                     /translation="MEKIDKLDRKILRIISQNARKPFKDVAEECEVSRAAIHQRVQRL
                     IDMGVITGSGYSVNPKMLGFQICVYIGITLERASMYKDVVAELEKIPEVVESQYTLGA
                     YTILIKMYAKNDEHLMELLNGKLQEIPGVATTETLTSLDQRIKRTIPIE"
     gene            9710..10705
                     /gene="trpS"
                     /locus_tag="PKH58_00920"
     CDS             9710..10705
                     /gene="trpS"
                     /locus_tag="PKH58_00920"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNX87617.1"
                     /translation="MKTVLSGIRPTGNLHLGNYFGALSNFVSMQNEYNCFFFIADYHS
                     LTTHPTPQNIQGNVKSVLAQYLAAGLDPEKATLYIQSDVPEVIELYLFLNMNAYVGEL
                     ERCTSFKDKIRQQPQNVNAGLLTYPTLMAADILIHKADFVPVGKDQEQHLEMARTFGN
                     RFNRMYNHEYFPEPQAFNFGKELVKIPALNGSGKMGKSEGEDSTIFLTDTPEVILKKV
                     KRAVTDAGPTEPNQPVSEPVANLFQLMHVVSKPETLEFFREQYSTCQIRYGDLKKQLA
                     EDMIAFVNPIRERILEIASDEIYLNKVAQIGKEKAQESARKTIREVREIIGFKPF"
     gene            11018..12538
                     /gene="purH"
                     /locus_tag="PKH58_00925"
     CDS             11018..12538
                     /gene="purH"
                     /locus_tag="PKH58_00925"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNX87618.1"
                     /translation="MENKKIKRALISVYHKDKLDEIIKKLYAEGVSFVSTGGTKSFIE
                     SLGIPCDTVEDLTSYPSILGGRVKTLHPKVFGGILARRDMEQDLAQLEQFEIPEIDLV
                     IVDLYPFEATIAAGADEATSIEKIDIGGISLIRAAAKNYKDVVILASEAQYVPFLELI
                     NQNGASTTLEERRWFAKEAFGVSSHYDSAIFNYFDGDNASAFRMSANGSKSLRYGENP
                     HQSGYFFGQFDDMFEQLHGKEISYNNLLDIDAAVSLINDFEDITFAILKHNNACGLAS
                     RPNLKEAWEAALAGDPVSAFGGVLITNAIVDKETAEEINKLFLEVIIAPDYDLDALQI
                     LTQKKNRIILVLLNNKVPKKQFRSVLNGVLVQDKDLHTEASGDLKVVTEKEPTAEEIE
                     DMIFANRIVKHSKSNAIVLAKGKQLIASGVGQTSRVDALKQAIEKAKTFEFDLEGAVM
                     ASDAFFPFSDCVEIAAEAGINAVIQPGGSVRDDESIEMCNKKGLSMVTTGYRHFKH"
     gene            12560..13579
                     /locus_tag="PKH58_00930"
     CDS             12560..13579
                     /locus_tag="PKH58_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNX87619.1"
                     /translation="MGLFSFTQEIAIDLGTANTIIIHDDKIVVDEPSVVALDKRTDKL
                     IAIGEKARQMQGKENEGIRTVRPLRDGVIADFFAAELMIRGMIKMIPSKNHLFSPTLR
                     MVVCIPSGSTEVEIRAVRDSSEHAGGRDVYMIYEPMAAAIGIGIDVEAPTGCMIVDIG
                     GGTTEIAVISLGGIVSNKSIRIAGDDLTADIMEYMGRMHNIKVGERTAEMIKINVGAA
                     LTDLEDAPEDYIVRGPNRMTALPMEVPVSYQEIAHCLEKSISKIEAAVLSALEQTPPE
                     LYADIVQKGIYLAGGGALLRGLDKRLTDKLNIQFHIADDPLHAVARGTGIALKNVDKF
                     GFLIR"
     gene            13681..14544
                     /gene="mreC"
                     /locus_tag="PKH58_00935"
     CDS             13681..14544
                     /gene="mreC"
                     /locus_tag="PKH58_00935"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNX87620.1"
                     /translation="MKNLISFLIRYSVVFLFIALEIISLILISNNKGYQRSVMLSSGN
                     AMVGRMYEMTNSFVEFFKLSEANDNLANENTQLRNRVVELENKLSTLTDTLNSVSWKN
                     VPVSPEKEYSYITAKVIRNTTHLSQNYITLNKGSNEGITPDMGVVGEEGVVGIVKSVS
                     PRFSVVIPILNPKIQISSKFKKNDYSGPLVWNGKDARFASLNDIARHVRFSLGDTLIT
                     SGLTKTFPEGIMIGTINDYNIKESDAYYNIQVKLAVNFRTLTHVKIIKYKNYREQSVL
                     EQTAADEGKKK"
     gene            14548..15045
                     /gene="mreD"
                     /locus_tag="PKH58_00940"
     CDS             14548..15045
                     /gene="mreD"
                     /locus_tag="PKH58_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNX87621.1"
                     /translation="MVSALQNILRFIFLILIQVFLLDNIQFMGYINPMIYVLFILLLP
                     VRFPRWALLISAFAMGLIIDIFSNTAGMHAFALVFAAFLRDPVINIFTSIDEGSNPTP
                     SFHTFGVSPFIKYVITIVLLHHITLFLLESFSFLNLSLIIPKILISSAITILLVLAIQ
                     SFKSK"
     gene            15046..16881
                     /gene="mrdA"
                     /locus_tag="PKH58_00945"
     CDS             15046..16881
                     /gene="mrdA"
                     /locus_tag="PKH58_00945"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNX87622.1"
                     /translation="MINDKLRNRGTVITIIMAVIVIIYILRLFSLQIIETKYKRYADS
                     NAFLNKTIFPPRGLIYDRNDSLLVYNKPAYDIAFINREIQGLDTASFCSDLNITTDFF
                     IEKMKEIKDRDKNPGYSSFTPQVFMTQLDIRDIANIQQSMYKYPGFYIQTRTLREYRY
                     NAGAHVLGSIGEVSQKKIDEDDYYNRGDYAGRDGIEYTYEKDLRGVKGVEILLRDARG
                     RLKGKYENGEKDIAPKAGKNLKLTLDIKLQLLAEQLLAGKVGSVVAIEPKTGEILAMV
                     SNPTFNPSLLVGRERSKNYNLLVNDPTKPLMNRATQAQYSPGSTFKPFQALFGLQLGG
                     ITEGTRFTCNGPSSWPIKCTHHHGSPVSLLNAIEQSCNPYFWNTFRSILEKDGYGNKN
                     VNFKKRFNKWRETVMEFGFGKKFEDTDIYQQSRGNIPSEKFYDKYFGKTGWRANTIRS
                     LSIGQGEILVTPLQLANGISAIANKGYYITPHLNKSDSMLTHKHKTSIEEKYFRVVDE
                     GMWRVNEFGTGRAYKIPGVSSCGKTGTVQNNRGKDHALFVGYAPRENPKIVLAVVVEN
                     SGYGATWAAPIASLLMEQFILHKIDRTELLNRISTSRTNVNVTDY"
     gene            16865..18316
                     /gene="rodA"
                     /locus_tag="PKH58_00950"
     CDS             16865..18316
                     /gene="rodA"
                     /locus_tag="PKH58_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNX87623.1"
                     /translation="MSRTISMKYALDWTTVGIYLFLVFFGWLNVYGASYDFDQSSIFD
                     FSQRAGKQFVWILTAFGLGGVILLIDSRLFNIFSYVFYVAIILLLIVTIFVASDVKGS
                     RSWLEFGPISFQPAELAKTATALALAKFMSSYNYKLKTWKDLIPLGIIVFIPFALIIL
                     QKETGSALVFLAFLLMFYREGMNGIFLLLGVLAIVLFVVAIGFSQTIIQTDRGTLGMV
                     LAMSIILLIQFCFAFFFNRHRKEALMLLLGVVGISAASYVLNIWFKINYDYIAIGTVV
                     ASCVYWIIIEMFNRHRKYWLLALISFGSLIFCYSADYIFNDVLEPHQQVRIKVLLNMD
                     DDLTGAGYNVNQSKIAIGSGGFLGKGYLNGTQTKLKYVPEQDTDFIFCTVGEEWGFIG
                     SAAVLLVYLWLLLRIIQIAERQRDPFTRIYAYCVASIFFFHLTINVGMVLGIMPVIGI
                     PLPFLSYGGSSLWSFTILLFILLRLDAARRIGS"
     gene            complement(18326..19642)
                     /locus_tag="PKH58_00955"
     CDS             complement(18326..19642)
                     /locus_tag="PKH58_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNX87624.1"
                     /translation="MNWNQLLSPVRLGMENYHDHNRDNRNEFQRDYDRLIFSAPFRRL
                     QNKTQVFPLPGSIFVHNRLTHSLEVASVGRSLGMNVSRSLSSAGSEYIDQIGFIVSAA
                     CLAHDLGNPPFGHSGEEAIATFFSEGKGQFLKNEMTEVEWNDCIHFEGNANAFRLLTH
                     QFNGRRPGGFVLTYSTLASIVKYPYSSVYADKKKQKFGFFESERIDFEKIANQLGITR
                     KADNPNRFNRYPLVYLVEAADDICYQIMDIEDAHKLKILTTDETKELFLGYFDDEKRK
                     KRVETLKMVSDVNEQIAYLRSSVIGQLIDECSEIFIENEKDILSGTFEGSLIKHMSGQ
                     TGKAYNHCSEIAKDKIYRSQEVLDIELAGYKIILTLMDLFVNAVLQPEKAYSRQLIYR
                     IPQQYDINNPTTYGKVLAVLDYISGMTDVYALDLYKKITGININSI"
     gene            complement(19701..20279)
                     /locus_tag="PKH58_00960"
     CDS             complement(19701..20279)
                     /locus_tag="PKH58_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNX87625.1"
                     /translation="MENEYAVNKIFEKIDFNQIPLKKGEYENCTFVNCNFNSADLSNI
                     IFSECLFTGCNLSMAVLTDTAIRETKFRECKMLGLNFENCNRFGLSFTAENCQLNHSS
                     FYQLKFKNAVFAHCLLHETDFTESDFTGTVFNDCDFSLAVFNHANLEKTDFRTSYNYS
                     INPENNRIKKAKFSLPAVTGLLDTYDIEIEDI"
     gene            complement(20304..21194)
                     /locus_tag="PKH58_00965"
     CDS             complement(20304..21194)
                     /locus_tag="PKH58_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNX87626.1"
                     /translation="MFKKIKPYMMPLAMTTGAVFHQFFGSLAFLTPYLIFTMLFLTYC
                     KLNFKHLQLSGLHLWLILIQILGCVIVYYILSPWNLVLAQGVMICMLAPTGTAAPVVT
                     GMLKGNVESLTAYSLLSNLSVAIAAPLIFSLLGSYETLPFFDSFLAISRRVFVLLLTP
                     FVLAIVLKKISPETTAKIGSFSGLSFYIWSFSLIIVTAKTVEFILQQNSNNYTTEILV
                     AAGALVACIGQFVTGKKIGKKYNDTIAGGQSLGQKNTILAIWMAQMFLNPISSIAPGA
                     YVLWQNIFNSYQVWQKRKLL"
     gene            complement(21257..21982)
                     /locus_tag="PKH58_00970"
     CDS             complement(21257..21982)
                     /locus_tag="PKH58_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX87627.1"
                     /translation="MLEISGSFHPMISDKDLGTITFRRSTRTTRISVRILSNSLVVTL
                     PPGSSETDALKFIDSVRPKLIKKQSGVNQKTVVISESNILKTCTFDVCIRKAERNNIF
                     SSLKSGVLTIEYPQQLNPEEMQTQTYFWNSINYFLRMEAKRILPQRTRELADQYGFSF
                     TDVKIQSSKTRWGSCNTRKNINLSFYLMLLPFHLIDYVILHELCHTREMNHSENFWHQ
                     MDLVTNGSSKKLRNEMKQYSMPK"
     gene            22063..22782
                     /locus_tag="PKH58_00975"
     CDS             22063..22782
                     /locus_tag="PKH58_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNX87628.1"
                     /translation="MDSWTQRTELLTGNNGLNKLRNSSVLIVGLGGVGGLAAEMICRA
                     GVGRMTLIDRDTVTSTNINRQIAALHSTVGQLKTEIIASRLKDINPVLELTVISDWLN
                     EENTDKILDEGKFDFVVDAIDTLSPKVFLIKSCTEKGIRIISSMGSGAKMDPSKVQIS
                     DISKTNYCPLAKAVRQRLSKLGIKKGLTVVYSTEESDRNSVILTEGELYKKSTTGTIS
                     YMPALFGLYLSSYVIREIIKN"
     gene            22788..23453
                     /locus_tag="PKH58_00980"
     CDS             22788..23453
                     /locus_tag="PKH58_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX87629.1"
                     /translation="MITAKNIHKSYGDLEVLRGVDLHVDKGEIVSIMGPSGAGKTTLL
                     QILGTLDKPNAGEVEVDGINFSKLNSDQLSVFRNRHIGFIFQFHQLLPEFTAIENVLI
                     PAMIGKMNIKQAREKATELLHFLQLGDRLHHKPSELSGGEKQRVAVARALINNPSLIL
                     ADEPSGSLDTKNKDELHKLLFELRDKYNLTEVIVTHDKELAKLCDRTIGMKDGLIQLT
                     IDK"
     gene            complement(23460..23531)
                     /locus_tag="PKH58_00985"
     tRNA            complement(23460..23531)
                     /locus_tag="PKH58_00985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23496..23498),aa:Arg,seq:cct)
     gene            23608..24249
                     /locus_tag="PKH58_00990"
     CDS             23608..24249
                     /locus_tag="PKH58_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNX87630.1"
                     /translation="MSKKQKYYTVWEGVNPGVYNSWKECEKQIKGYEGAKFKSFETIK
                     EAQDAFMSSCWEYIGKNAVNTKPTPNYRNLPSSEQPKLNSIAVDAACSGNPGKMEYRG
                     VDVNTGIEIFRQGPFENGTNNIGEFLAIVHALALFNKTNPHIPVYTDSKTALAWVKAK
                     KSKTKLGMDEQNQYIFQLIERAEKWLRENKFNNPLIKWETEKWGEIPADFGRK"
     gene            24258..25082
                     /locus_tag="PKH58_00995"
     CDS             24258..25082
                     /locus_tag="PKH58_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87631.1"
                     /translation="MNRIKKCCLLSLLSLFALLQAGCSDNITPDQPLVVIIKADDFND
                     YSPNWQRFIAILQQNDISAAAGVTSTLISSNHSAIIKIRELSGLRRSNNQPLIEFWNH
                     GYDHAGDNGDSPEFDNSDVDLQKKHLQLAQRFFSDSLHIIAKTFSAPHNRSTTATLHA
                     LSCIPELNVIMRYKNHEKFSLKRPWIDPNKKKFKPGDKICLNIKFQYLLDIPSTDVEN
                     YFKAGEKDNYLLIQLHPNPWDENDFNDFQKMISFLKGKNVKFMTPDEYFQYLDVMN"
     gene            25098..26543
                     /locus_tag="PKH58_01000"
     CDS             25098..26543
                     /locus_tag="PKH58_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNX87632.1"
                     /translation="MKLSFKHNYFSLFLLLFTTLAMVAVHRLYSTQVYVTKENVSLFG
                     NIILLIFALSSVLIFISQIILVRRILRPFLSVENFVSLSLVLLLVLSVFQKEVGIYAT
                     GFFVLFALVSFFVRGKIYTLNKVYYFIFLYAVFLMAGTIYTPRGFHFPEMTYTFYLLP
                     LSFSLFRLNKQTLLLILKIVFRIMAVYMAVSVVYWWFNLLVLNKSALEWITMKFPNAY
                     DYVGTWGHYSHPSYISLVLFSALISGFYLNFKMDNTVTLSKFELITYIGLCLGFVLVT
                     ESRIGLVELIIIVLISLLYYIRLKTPHFKLALVVAVLVGVVILMAGENKVSRFIRDDV
                     RKADYTLAVNYIKEHPVIGAGYHQQRAALEYQDKKMSDVTRPEGAPPITYTHNQFLGD
                     MVQFGIPGLICLLILLTGLIWNALRSRSYLLQLFMLIYLFFMLIEEPLYAQEGITRFM
                     SYLCLFVHISECEKPLKAFNLRNLFSKSKTT"
     gene            complement(26508..27296)
                     /locus_tag="PKH58_01005"
     CDS             complement(26508..27296)
                     /locus_tag="PKH58_01005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNX87633.1"
                     /translation="MHSEPKISVITPIHNREKYIRETLECLQKCNYKNWECIVMDDDS
                     TDNSAMIIKEMARTDSRIKYFHQPKSPIPVTKNNAVAHSSGKYILPLDSDDLIAPEYI
                     AEAVEILENNTSVKLVYCSGVFFGDKKGKWKLPAYNFRQLLLKNCIHNTAVFRRVDFD
                     SAGGYDPNIITSEDWDLWISLLEKGGEVYFIDKEYFFYRKHTDSFTDNHQDKIAATQM
                     QIYANHKEVYDRLIDNPIQFLRDYEKYKKKYNQLRRLTFRKQIP"
     gene            complement(27399..28166)
                     /locus_tag="PKH58_01010"
     CDS             complement(27399..28166)
                     /locus_tag="PKH58_01010"
                     /inference="COORDINATES: protein motif:HMM:NF017515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HNX87634.1"
                     /translation="MEYINNPSELTYQHNVPFVVWCYWEGAPMNANRLLSYEYLQKNI
                     QVPICLISKENLAAFIKPEHPLHEAFPYLSVVHRSDYIRAYLMHFYGGAWHDIKATEV
                     SFKNCWYEFENPDIYLIGRPENQKGAARVYDEHNRYMPDYWKELVAVPAWIAKGNTPM
                     TAEIYTAFLSLLDTNMESLKKHPAKHPREKFIQPKNIFHAVIIRLKQLYSGRNPYYPL
                     PWTVFGNIFHPIVYKYRKHISKNLPVNKIKNAGIYHR"
     gene            complement(28208..29101)
                     /locus_tag="PKH58_01015"
     CDS             complement(28208..29101)
                     /locus_tag="PKH58_01015"
                     /inference="COORDINATES: protein motif:HMM:NF025089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX87635.1"
                     /translation="MISVIICSKHEKLSESVRQNINNTIGVPYEIVCINNSRNEYSIF
                     SAYNKGIAESKYPFLCFVHEDVNFQTKNWGNIIKSHLNNSTTGLIGVAGSNTVLSVPS
                     SWSSSQEKYINIIQSEKHSEHVRLPLEHNDDKQEVILLDGVFLGANRNIFSKITFDEK
                     LKGFHGYDLDICLQSAFAGFQNYVVFDILIEHYSKGFRNKRYFENLIQVFGKWEKFLP
                     IYDKNKYTAEEILKSEEKMINYLFIKMIKRGFSAKEIKSTITFYIDKIKSEKLKIKYR
                     HINRKFFFLQLVYKPFTRLYL"
     gene            complement(29112..29918)
                     /locus_tag="PKH58_01020"
     CDS             complement(29112..29918)
                     /locus_tag="PKH58_01020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX87636.1"
                     /translation="MKASLIISVYKNIRFLKPVLDSLHFQTEKDFETIISEDGESPEM
                     KEFIAGYPFEMPYKHITRKDEGWRKNHCLNSAIRASEADWLIFIDGDCVLHPRFIEMH
                     LHHAEVNKILAGKRVKLDDYMTEYILDNSDNIRNLQQILLRKMFFGKGKIKFIEEGIF
                     ISPESILRIIPRNRKNDKLVGCNMSFSKEAIYAINGFDEDYKLPAVGEDYDLAWRLKA
                     AGFQLVSMRNLAVQYHLSHPEGYNQDFNLNLCRQKQEINEFVCKNGLDKH"
     gene            complement(29922..31034)
                     /locus_tag="PKH58_01025"
     CDS             complement(29922..31034)
                     /locus_tag="PKH58_01025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNX87637.1"
                     /translation="MVNIKPANDIHKASESENFPLVSVIIPVYNSELYLAETLESVLI
                     STYPNFEVIIMDDGSTDKSMEIAQNHALQDARIRCFVQPNAGASVARNHAIGLSHGKY
                     ILPVDSDDTISNNYIEEAVKVLENKPEVKVVSREASFFGEKTGRWNFPPFSYNLLARR
                     NLIDVCSMYRKADWDKTGGYCEEITGREDWDFWISLFKTGGEFVRLPINGLNYRIRSG
                     SKRVKTRNLKKELIDMLNIRHKAFFYRELKGKLHYQRTHSRKLNIIRHFFLPENIFVN
                     PEYSVYENAVYLSNEEISIRNNQITKLFPSVAEKIKITEFTEKRYHIPGNGIKTSKAR
                     DMYRQTISDSYKFCLGYYEQQISGCTLKSFLIILEK"
     gene            complement(31036..31749)
                     /locus_tag="PKH58_01030"
     CDS             complement(31036..31749)
                     /locus_tag="PKH58_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX87638.1"
                     /translation="MNIHIITPVKNSFGTTIRTIENIVGSEGYSDLKYTIYNDFSDDE
                     TTFLLQEASSRFGFELVNLKDITSHPSPNYRLVLQLAQKKAIDDNAHLLIVESDVVIK
                     NDTIRQLILQSGKLKNPGLIAAVTTDESGKINFPYLFAQNFREGTVETKKRLSFCCTL
                     LSNQLLNAYDFTNLDAQKTWYDIFISHKSTSMGFRNYLLTEAPVLHLPHSSRPWKKLK
                     YTNPLKYYWQKYTKGLDKI"
     gene            complement(31746..33242)
                     /locus_tag="PKH58_01035"
     CDS             complement(31746..33242)
                     /locus_tag="PKH58_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNX87639.1"
                     /translation="MAKEMKSLAKDTVIYGGTNIAAKFVSWMLVVLYTYTLPNPAEYG
                     VVTHLYAWTSILIVALTYGMETGFFRFANKDTANAKKVYGNALISIGFTSLLFAIIII
                     VFQQPIANTLGYRNHPEYIWMLGLSVAMDAFACIPFVYLRFIKRPYVFAGMKLLYVGL
                     NVVLNVFFLVLCPILMKAAPSTISWFYRPDYGVGYIFISNIISTTLQTLILSRYIFEA
                     EFKFDSQLLKQMLKYSYPLLFLGIAGIANQNLDKMIFPFLQVGSKGGNDLGIYGAVSK
                     LALVIMMFTQAFRFAYEPFVFAKHKDENSSQTYSEAMKYFVILSFLIFLGMILYMDII
                     KYLVPSEYWVGLKVVPIILFSFIFQGIYFNLSIWYKLKDKNHYGAWFAAIGTLIVFVG
                     NIIFVPKYSYWGSAWSAFAGYFIVMLLSYFFGQKFMPINYNLKKLGIYFALTLVLFGI
                     STFIETRYLIVNLALKTVLMAVFIVFFIKRDLPLKDIPVLNRFLKKQA"
     gene            complement(33282..33569)
                     /locus_tag="PKH58_01040"
     CDS             complement(33282..33569)
                     /locus_tag="PKH58_01040"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNX87640.1"
                     /translation="MDELVNIRTFSQSADFEMVKAYLESMGIECFGRDEIINRAYLAN
                     VNGGVKLDVRASQAEEAIRLLIEGGYLKPEDFEPTPELKFVDKILGKFRKK"
     gene            complement(33589..34608)
                     /gene="ruvB"
                     /locus_tag="PKH58_01045"
     CDS             complement(33589..34608)
                     /gene="ruvB"
                     /locus_tag="PKH58_01045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNX87641.1"
                     /translation="MEEKFDIRNNKNTEKEFENALRPLSFSDFKGQNKIVENLRIFVQ
                     AARMRSESLDHVLLHGPPGLGKTTLSNIIANELNVGFKITSGPVLDKPGDLAGLLTSL
                     ESNDVLFIDEIHRLSPIVEEYLYSAMEDYRIDIMIDKGPSARSIQLELNPFTLIGATT
                     RSGLLTSPLRARFGINCHFEYYDTQVITGIIERSARLLNVQIDHKAAQEIAGRSRGTP
                     RIANALLRRVRDFAQVKGNGTINPEIADYALEALNIDKYGLDEIDNKILTTIIDKFQG
                     GPVGLTTIATALSEDPGTLEEVYEPFLIKEGFLKRTPRGREVTELAYKHLGKVKFGGD
                     MGTLF"
     gene            complement(34659..35603)
                     /locus_tag="PKH58_01050"
     CDS             complement(34659..35603)
                     /locus_tag="PKH58_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX87642.1"
                     /translation="MSIQISHISRSFGNQQVLRDISFRIDAGEVVGFLGPNGAGKTTT
                     MKILAGALDYQTGNAAVCGMEVADNPLETKKNIGYLPEQNPLYTDMYVKEYLLFVAET
                     YGIGKEKYKRVDELIDLVGLRPEFSKKIGQLSKGYRQRVGLAQALIHNPKVLILDEPT
                     TGLDPNQLVEIRNLIREIGKNRTVLFSTHIMQEIEAVCNRVIIINKGEIVADYTDVKE
                     ISNFGKNDFQLEVEFLNKVEKDVLGSIKSIKNIDTKDSKLFSITSEKDIRSDIFDLAV
                     KTNNKILTLKPLAKRMEEVFRELTIAPESPGWDFRNKQ"
     gene            complement(35606..36286)
                     /gene="pssA"
                     /locus_tag="PKH58_01055"
     CDS             complement(35606..36286)
                     /gene="pssA"
                     /locus_tag="PKH58_01055"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNX87643.1"
                     /translation="MIKHIPNTVTCLNLFSGCVGVWFAFNGNYEGVALAVIFSAVFDF
                     LDGMAARLLRAYSPMGKELDSLADVISFGLVPGALIFHMLSPDLSHNYAFVGFLLTIF
                     SALRLAKFNIDERQTSSFIGLPVPANAIFWAGIALAWSEWFIANPYILLGLTVFFSYL
                     LVAEIPMFSLKFKNLSWKDNKVQFIFLGGSVLLIAILWKKAISPLIMWYILLSVIVNF
                     IQVKKAEN"
     gene            complement(36394..36846)
                     /gene="dtd"
                     /locus_tag="PKH58_01060"
     CDS             complement(36394..36846)
                     /gene="dtd"
                     /locus_tag="PKH58_01060"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNX87644.1"
                     /translation="MRILIQRVKRASVTIENHLKSEIKTGLLVFVGIEDADDEKDSSW
                     LASKLVKMRIFNDEKGVMNLPVTEVDGEIMIISQFTLHAQTQKGNRPSYIKAARPEKA
                     IPLYEHFCNEVEQLLKKPVKTGEFGADMQVELINDGPVTIWMDSKLSD"
     gene            complement(36877..39147)
                     /locus_tag="PKH58_01065"
     CDS             complement(36877..39147)
                     /locus_tag="PKH58_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNX87645.1"
                     /translation="MKFSKGSLFLILVLGLLASACNPTKYVPDNEHLLNDVKIETDNK
                     AIPKEDISTYIRQTPNNEIFGIYKMQLGIYNLAGADSSKWINRRLKKIGEEPVIYNPS
                     LTTITEKEITKLFSNKGYMDVKVNSEVSFPKEKLADVKYSIQSNEPYRLNSYSVNINQ
                     PELSDIASDTAASLIKTGKLFDSDVFDEERDRITSKFRELGYYNFTKDLLHYYADSSL
                     VSHRIDLVLEMNEQLKKSDESIKERIFQQYTINKVHFYCESDKSTDAVNLTPELDTLV
                     LGDYELVYENKRNIRPTVLIEHTLIVPGKPYGDRSVERTYSSLNSLSSIKYVNVSFRE
                     IANDKLDCYIVITPNKLQTISADGEITYSSGYWGFGGNINYGHRNAFKGSETISAKGR
                     LAYEYQGTGQKAIEVGTDLGLKFPTFLLPFSSLDMRRNIRANTEITATFSYRDRPKEY
                     TGIVTGTGFKYSWSEKLQVKHNFDVLDISYVNYPKITGEYQNYINSNPAFAYNFQPHL
                     IMRTGYNGSYSGFNAAQPLRNYTTMNYGIETAGNILQGLNSVLNAQRDSTGAFKVFGI
                     RYAQYVKTDFQIAHYQIFDANNRLVYHAGLGIAVPYGNAERMPFEKRFFSGGANSVRG
                     WTAYQLGPGTYRSPGNYVDYNTQMGDIKLDLNMEYRTKLFWKLDGAFFLDAGNIWTIR
                     NYEAQPGGVFRFDKFLGQLGIAYGTGIRADFSFFVLRVDLGVRLFNPALERTEQWRTN
                     IKAEDFALNLAIGYPF"
     gene            39280..40044
                     /locus_tag="PKH58_01070"
     CDS             39280..40044
                     /locus_tag="PKH58_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX87646.1"
                     /translation="MISKSKIKWIRSLEMKKNRDEQNLFVAEGVKLVSDLLPHLRCRY
                     LAVCTDSFDIERFSRFAAENEQITTDEYKKITFQKSPQGVLAVFEKPAHLWDEKETCS
                     KLSLALDDIQDPGNLGTIIRLADWFSIEHVFCSTHSADVFNPKTIQATMGAIANVKVH
                     YVELPLFLRQLEGRIPVYGTFMDGENIYDRNLQRNGIIVMGNEGQGISAEVEQLVTER
                     LQIPCFAPGTVASESLNVGIATAITVAEFRRQTYKK"
     gene            40051..40329
                     /locus_tag="PKH58_01075"
     CDS             40051..40329
                     /locus_tag="PKH58_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87647.1"
                     /translation="MRKTTYFIILTVIALVLFAWATNPNEQQHQEAARIKFGEIVENT
                     MADLGLKKNLLTDLGFEIGNRYIDSLLDDCISANNYLVCSTTAFTING"
     gene            complement(40446..41540)
                     /gene="proB"
                     /locus_tag="PKH58_01080"
     CDS             complement(40446..41540)
                     /gene="proB"
                     /locus_tag="PKH58_01080"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNX87648.1"
                     /translation="MDKTNIRYKKVAVKIGSNVLTRPDGTLDITRMSALVDQISVLHK
                     LGVQLVLISSGAVASGRSVMGISKKMDIVEQRQLFSTVGQAKLINHYWELFREHGIVV
                     GQVLTTKTNFEGRSQYLNQRNCIRIMLENKVIPIVNENDAVSVSELMFTDNDELSGLI
                     ASMLGMEALIILSNIDGIYNGSPSNPESAVIRKIRKGEDISDFIQTSKSTLGRGGMQT
                     KTSIARKIADEGIEVIIANGKRENILIDLLDKQKDTVCTIFEASATDVSSVKKWIAHS
                     DGFAKGEIHINENAEKALSGPKATSLLPVGVTQITGTFEKDDIVKIVGSNGKIIGVGK
                     VQYNNEKARQLIGKKNQKPLIHYDYLYIED"
     gene            complement(41548..42516)
                     /locus_tag="PKH58_01085"
     CDS             complement(41548..42516)
                     /locus_tag="PKH58_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNX87649.1"
                     /translation="MRNLLFISLIIMAFSCSSTGNKTENKAETVQVSVPEFSADTAYK
                     FVKTQVDFGPRVPNTAQHKACGDYLVSALKQFGATVTVQEASLTAFDGTTLKSKNIIG
                     SYNPDAETRLLLFAHWDTRPWADNDTDEKNHKTPVLGANDGASGVGVLLEIARQLNRQ
                     KPSVGVDIIFFDAEDYGASAGNEDSWCLGTQYWAKNKHIPNYRARYGILLDMVGAPQA
                     KFYREQLSDNYAKYVVDKVWSKAAELGFSNYFVNKPGGAITDDHLYVNQLAGIPAIDI
                     IQYDPYSMTGFGKYWHTTNDTMENIDPVTLKVVGTTLLHVIFNEKP"
     gene            43176..44048
                     /locus_tag="PKH58_01090"
     CDS             43176..44048
                     /locus_tag="PKH58_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNX87650.1"
                     /translation="MSFNIVVLAKQVPDTRNVGKDAMKADGTVNRAALPAIFNPEDLN
                     ALEQALRVKDKIKDATVSVLTMGPGRAAEIIRESIYRGADNGYLLSGREFAGSDTLAT
                     SYALSCAIRKMGKVDLIVCGRQAIDGDTAQVGPQIAEKLGLPQITYTEEILDVDAASL
                     TAKRRVERGVEVVKGPLPMLVTVNGTAAPCRPRNAKLVMKYKYAKTSTELQESSSDYT
                     DLFNSHPYLNITEWGAADIEHDPQWLGLSGSPTKVKQIESVVFTAKESKRMGVSDTEI
                     EDLIKELIANHTIG"
     gene            44068..>44191
                     /locus_tag="PKH58_01095"
     CDS             44068..>44191
                     /locus_tag="PKH58_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HNX87651.1"
                     /translation="MNNIFVYLEIEDGVVGDVSLELLTKGRSLADQYKCHLEAVA"
BASE COUNT        13205 a         8564 c         8896 g        13526 t
ORIGIN      
        1 tttaagaatt tatacaagca gagtccgaac ttatattttt acatatgttt ttactacacc
       61 gttttgtgtt gttgatattg ttaccttata ccctttattt gttagaaagt cggctatcgg
      121 aactggtggg aagggagtaa gaatttccag acagtccccg gatttcatgg ttttggccaa
      181 ttcaaatatg gcatctttcg ggtggtcgcc agcatcaatt accggccggg catcatagtg
      241 atgagtgact ctatatgaag gaatttcggc gttgatatcc gaattttcta tttgtgcata
      301 ctcatcgtgt aatggtgact gacctacttc tgccctaagg gtatcaacca tttccacaat
      361 cgaaacatta ccaaccttag ctgcttgttg caatgaagtg accttagcta cagtacggcg
      421 taataccgga tttttcaatg ctgaaaatgc aggtgaatac ctgattattg tagcttccag
      481 ttgtggaaac tcttttaata aatcagcaat ttttgtatcg ggagttatgt tcattatttt
      541 aataaaaaag tttagtattt aatttgatgg taaattttca gtttaagaac aaaacaggcg
      601 gctaatgccg cctgattatt tttctatttt tgaataagag agtagtcttt gatcaccttc
      661 cagttttctc aattcagtaa tatcctgagt aatttcaaca actccaaaat attcgccttc
      721 aggagaacga acagctgtat attctatata aataaaccgt cctttgaaat cagacatcca
      781 aaacgcggct ttgttttctt ttccactttt gaaatcggtc agtatttgtt ccacaatatg
      841 tacgcttcct ggagggtggc acatacgtac atcacggcca agtattgatc ggttacgttc
      901 aaacacacga ttggggctgt gcgaaaaaaa ttgtacttta tcgttcttat caacaaatgt
      961 aatgtcaatg ggtaaatgaa taaacagagc ctctaattct tccatgctaa atgccccgga
     1021 tgacaaacgg attgcatctg tatggataaa ttcaggctgt gtttcggcaa catcctccgg
     1081 tttccattct gcttcaggat caaccaggca gaaaccaaat tcaggtgttt ctttatatat
     1141 tttgtaccat tcctcactgg tgagggtatc cattgtcatg ggtaaaagaa tttcttcctc
     1201 tttcatgatc atcccctcaa taagttccag gacaggctta ataaccattt caatgattgg
     1261 ttttagttcc tcgacgtcaa tttcgggtgt ttccagtgcc tcgtaaatat ctttcatcaa
     1321 accacgaatt tcatcgtgtt ttccccacat tacttttgga ggtccggtta cttcgtgttt
     1381 ctccaggtaa ggaaaaagca ggtattcttt acgtttgtaa tgcttgtcga tatcggataa
     1441 agcattgaaa actgtgcgaa ttttaatcac atattcgtta atgttttctt tggttacact
     1501 ttttagatct tcaaaaagat ggtgtgcttt atcaatttca ttacggatag ctatattctc
     1561 ctttttgaaa gtatcgacag gatgaccggg gggaatagtt ttcgcgcctt ccaaatcaat
     1621 gctgccatcc aatacggatg tatgtaaatc gcagaaatca agaatttctt ccgatgtgag
     1681 agttcctgaa ttgatgagtt cctcttcgac ttccactact tcattgtaag gaatgctgct
     1741 taatacttca ataagtcttc cacgcaagac tgaggcaggt gcgccttcat gcagttgaat
     1801 taacagatgc ttaagcaggt cttttctttt tgttgagtta tctatatatt cgctcataat
     1861 gaggttttta tattgttata attaaccttt tatcatgatt aacagcattt tgaatggttg
     1921 taccgcgttt agcgcatgcg gatgattggc tggcattatg atgcattctc cctttttaac
     1981 tattttgggt tcaccatcaa tacgtatttc agcttcacca tcgagtatct caactatagc
     2041 gtcgtatgct gctgtgtgct ctgtcagacc ctgtcccttg tcaaaggaaa acagggttac
     2101 gttgcctgcc ggacttttgg ttatttgttt gctgacaacg ccaccgccgg tgtattctat
     2161 cgaagtttcc aaaggaaaaa cttcgccatt attaaacaag gtattcattt tgttttcctt
     2221 attgtaagta aatttcatat tcatttttct gataatttgg tagagcaaag taatagttta
     2281 gaattaatat aaattgtaac caaagttaca cttttcgctt ttataatatt ataaaccaga
     2341 aataggtttt tttgttctcg taaagtgcta tcgaaaagtt tttttaggga attatcaaaa
     2401 aattaactaa ttggttacca gaattgttag ttagtaatga taatctatat tattatcagc
     2461 aacgtgaatt tgcaagaaac aattatcaga ataaatattg tctttttata tttcagaaaa
     2521 taacataaag gatgtctgta aaggaaggtg ataaatagaa aaactgaaga ttttttataa
     2581 ttctggtctt tatgcccggg aagtctgttt ttttaacgca tattaatcgt ttttttggaa
     2641 atagcagttc tggtcaatct gcaggattta tattttccag ttccaccatt tgagtttttc
     2701 aggttcacgg ttttcaggat cagtttcggc aaaatagaca gcacaccgga aaacgtaaag
     2761 caagcatcga tcctgtacgc agcctgccat ctgattagac caatcgtaca aatcttccgg
     2821 atttttgaat ttcaaatcat caatttttcg tatgccaatg cggtataaat cgttagctat
     2881 ggatggacca acgcccggga tttgtcgtaa ctctctgatc gatgcgggtt tattttccat
     2941 attatatttt aaaatgaaag acacttaaga gatttctccg agctgtaagt cagggaaata
     3001 catacttaag tgtctgtttt tgttttaaga taagtattaa atatcctttt cggcaatcag
     3061 gtattcggca atctgtactg cattgagggc tgctcctttc ttgatctgat cactaacaca
     3121 ccagaaggta atgccgttgg ggtttgaaag gtctttgcgt acacggccaa catgtacggg
     3181 atctttacct gataggaaaa gtggcatcgg atatattttt tcagccggat tatccatcaa
     3241 tactacaccc tgagcatttg caaatgcctc gcgggcttct tccacgctaa tcggacgctc
     3301 tgtttctgcc caaacgcttt cggagtgagc gcgcagggtg ggaacacgaa cacaggtagc
     3361 gctgacttcc acatccgaat gcatgatctt gcgcgtttcg ttgtacattt tcatttcctc
     3421 tttggtgtac ccgttttcgg taaatacatc aatatgaggt ataacgttat aagcaagctg
     3481 ataagcaaat ttactcactg ttggttcttc acccttagca atctgacggt attgcagttc
     3541 cagttcgtcc atggcggcag ctccggcacc gcttgcggcc tgataggatg aaacatgaac
     3601 ccgtttgata tgtgatacat cttcaagcgc cttaagagcg acaaccatct ggatagtagt
     3661 acaattagga tttgcaatga ttcctcgtgg acggtttttt gcgtcggcag gattaacttc
     3721 tggtaccacc agcggtatat cgttttccat acgaaaagca cttgaattgt caatcattac
     3781 cgcaccgaat ttggtaatat ctgctgcaaa ttcttttgaa atgcccgctc cggctgatgt
     3841 aaaagcaatg tcaatgccct tgaaatcgtc accgtgaact agttctttta caatcagttt
     3901 tttaccttta aattcgtatt ccttacccgc actgcgtgaa gaaccaaaca aaaccaattc
     3961 ggtcaacgga aaattccgtt catccaatac ccttaaaaat tcttgtccta ctgcgccgct
     4021 ggcgccaaca attgctactt tcatactttt atatttttat gtaatttctt ctaatttaaa
     4081 attataacta tatttgtctt cttattgttt tccctgtcaa aatttctgca aaattaattc
     4141 atttcaacta atttactgca ttttatgaag aaatcgttga taattttaat aattctgttg
     4201 cctttttttg ccttttcgca gataaatgaa actttttcgg atggtaattt cactgaaaat
     4261 cctgtttgga cagggactgt tcaaaatttc agggtaaatt cagaaaatca gcttcagtct
     4321 caggcggcag ctacctcaaa atcttatctt tcaacccagt cggaggcttt tgaagatgca
     4381 acctgggaat tttgggtcaa aatagcctat acaacctctt catccaatta tgcttcggtt
     4441 tacctgattt ccgatcgtgc tgatgtgact accgaatgtt acggatacta tgttcagata
     4501 gggaatacca acgacgaaat ttcgctttac agacaggaag gaacaacgaa gactaaaatc
     4561 atagacggta cagataagtt gatagatgca aatccggttg aagtacgggt gaaagttacc
     4621 cgtgacaaat ccggaaaatt caccctttat cgtaaattgt catcagacaa tgattatgtg
     4681 acagaaggag aagaagttgt ggataataaa atacagggaa gtaaatattt ttcactgttg
     4741 tattccaact cttctaccac cggaaatgcc tatttctttg atgatatatc cgttgccggt
     4801 gagaaactga ttgatgtaac tcctcccgtt tggacatctc tgtcgcttat tcagccggat
     4861 aaactaatcc tcactttctc cgagccaatt gattttacaa gtactgaatt tcaggttgat
     4921 aatggcattg gaagtccttc caaacaaata gtttcggctg ataaaaccac agtcgaacta
     4981 acttttacca acaattttga gaagggaaaa atatatgtgg tcgaagcgtt gaatgtaaaa
     5041 gacatctccg gaaatgcatt ggaaaatcct cagaaacaaa tcgggctggt tgaacctatt
     5101 gaggttggtg atttgattat caatgaaata cttttcgaac cggctgaaga tgttccggag
     5161 tattttgaaa tattcaacaa ttccggtaaa gtgctggatg tgagcaaggt attttttgga
     5221 acacgtaaaa ccgacggttc attcatacct gccaataatt ttccaccaaa aaccctgtta
     5281 ctgccacttc agtatctggc attaacgccc gatgcaaatg caatacgtaa cagatatcag
     5341 gcacccgata ctgccagaat tttaagttcc tcaaaatggt cggcgttaaa caatagcagt
     5401 gcagctttgc tcatcaccaa ttccacagga gacaccattt atgatgaagt gaaatatgat
     5461 gtcaagtggc atcacccgat ggtaagaatc accaccggag tttctctgga acgaatcaat
     5521 ccttcacttc cagctcaaaa cgcaggaaac tggcactctg ctgcatcgga ggttcagtat
     5581 ggaacaccag gttataaaaa ttcacaatac agagatatct catcaaccaa aacaaccgaa
     5641 aagtgggtgc gaacagatcc agaaagtttt actcctgata atgacggaat ggatgatgta
     5701 tgtttcatcc gttatataac cgaaggaaat ggatttacag ccaatgccat gatattaaat
     5761 tctgtcggtg taaaagtacg tcaagttgca gcaaatcaac tactttctac ggatggattt
     5821 tttacctggg atggacgaac cgacaacgga aaaagtgtaa atcctggcat ttatattatt
     5881 tattttgagt tgattaatgc aaattcaggt gttaaaaaaa cagaaaaaat tcctgttgta
     5941 gtttcagccc ggtaaaaata gaaattttat acagtctata attgaaataa ataatctatc
     6001 tttgcactcg ttttaactat aaaaattaac gatttaataa agatggataa agtaagttat
     6061 gcacttggtt taagtttggg taataatttg ttaagtagtg gaattactgc gctgaatgta
     6121 gaaaaattca gcaagggtgt tcaggatgtg ctcgaacaaa accaaccgga gatttcatat
     6181 caggaagctc aaaacgtaat aaacgaatat tttcaaaatt tgcaggctaa attaagcgaa
     6241 aaaacgattg cagaaggaaa ggcttttctt gaagaaaacg gcaaacgttc cgaagtaatt
     6301 acgctgaaaa gcggattaca atatgaaatc attaaagaag ggaatggctc tgtacctact
     6361 gcaaataata cagtaaatgt tcattatcac ggaacattga ttgatggcac tgttttcgac
     6421 agttccgttc gccggggcga accggctacc tttggagtga cacaggtaat tgcaggttgg
     6481 gtggaagcac tgcaactgat gcctgtcggt tcaaaatgga aattgtatat accatccaac
     6541 cttgcatatg gtgctcaggg agctggtcag catatcggac ctcacactac attaatattc
     6601 gaggtagaat tgctgggtat tgagaagtaa acaactaatt atcaattgta tatatttaac
     6661 tatcacaatg aaaaagattt caatactacc tttggcagcc ttattgctgg ttggctgttc
     6721 ttctgtaaaa aaagtggaaa ctccggttgt accggttgct ccggtaaaca ttcttacgaa
     6781 cgatgttgac tcgatgagtt atgccttagg agtaaatgtc ggagaagatt ttgccaagaa
     6841 tattaaaacc attcctggtg ggaaatccaa tatcgacttg ttaatcaaag gttttgcatc
     6901 ggccatgaaa ggtgattcta cccttctgtc gaaggatgtg gcgcaaacct attttcgcga
     6961 ttatctgact aaagctcaaa acaaacaaaa cgacctggtt aaagcagaag gagaaaagtt
     7021 tttggctgac aataaattaa aagaaggagt acaggtaaca tccagcggac ttcagtatat
     7081 aattctcaaa acggcagaag gagcaaaacc tcagtcaact gacaaagtta aagtacacta
     7141 caccggaaca ctgatggatg gaaaagtatt tgacagttcg gttgaacgtg gcgaaccggt
     7201 tgaattccag cttaatcagg ttattcccgg atggactgag ggtgttcagc tgatgccggt
     7261 aggttcgaaa tataaattct tcatacctta caaccttgcc tatggagaac agggagtgtc
     7321 tcaggcagga attccgcctt tttcaccact tactttcgaa gttgagctgc tcgatataat
     7381 aaaagaacag ccagcacctg tggtagaaga agtaaaacct gctgctgaga caaaaacacc
     7441 tgcaaaaaag tcaacaaaaa agaaataatt aataaataat taataaaaaa atgaaaaaac
     7501 aaaattttct gacaatggca cttattattg ccattttggg ccttgcttca tgtggaagca
     7561 attataaagc aaagaaagct aatttatcaa ctcaggaaga cagtttaaat tatgcgttgg
     7621 gtctggccat cagtgaagaa ctccgcatga atgcacttca aaatgattcg tccgaaaaaa
     7681 ctattgctgc tttggttgaa aagattgaaa aagtctatag tgacgaaaac actgatcagg
     7741 tttacaaaac cggcttccaa atcggaaata tgatgaaaga acaaaataaa gtgggactca
     7801 ttggtgattc tactttgaaa tttaaccagg atttattcaa aaaaggtctt gttaaaggaa
     7861 ttaatggtga tactaccggt atgactggcc cacaagcgat ggtttatttc cagaatacca
     7921 tgcagaaaat tcaaaatgaa aaaatgatga aatctgcacc tgcttcagct cctgcacccg
     7981 ctgacaccat caaaaagtaa tcattcatca ataattaata attaaaaaaa tgaaaaaaca
     8041 aaatatattt ttcgcagtag caattgttgc tatcacactg ctgacagctt gtaacggagg
     8101 taaaagtggc agtacaaaag atccaaagtt agcatcacag attgacagtt tgaattatac
     8161 ttatggcttg gcttttggta atgaccttaa aaccaacatg tttaacaaag actctgttga
     8221 gcaaaaaaca aaaaccttta tgaaaggtct ggctgaagga gctaaaggcg aaccggatgc
     8281 aaaaccgggt ttatctgcag ctgcaaatca gtatggcgaa tggttgaaac aacaaaagac
     8341 tgccggtttc ctgggagatt ctaccctgaa agtcaacttt aatttattca aacaaggttt
     8401 gatcaacgga ttgaataaat ctaaaattca gatgacacct caagaagcca atgaatatat
     8461 caacaaggta atgaatgccc gtcaggaaaa aatcatgatg aaacaattcg gtaaaaacgt
     8521 ggaagcacag gataaatttt tagctgaaaa tggcaaaaaa cccggcgttg ttactaccgc
     8581 aagcggtctt caatacgaag tgattaaaaa aggaacaggc ccgatgccaa cagctaccga
     8641 tcaggtaaaa gttcattatg tgggtacact gctcgatggt actgaatttg acagttcaat
     8701 taaaagaaac gaacctgcta tgtttgcagt taatcaggtt attccgggat ggactgaagg
     8761 tcttcagttg atgccggttg gatcaaaata taaattctat gtacctcaga atcttgctta
     8821 tggttcaaaa ggtgcaaggg gtattgaacc actcagcatg cttatttttg aagtagaact
     8881 tctttcgatt gagaaataag cgttaagcaa taaataaaaa aagcaaccgg acattttttg
     8941 tccggttgct tttttttaca taaatatatc gaaaacgcat tttttacaaa ataatttatt
     9001 gaaatatgtg tttttattgt aaatatgttt tgattataac aaaaattata tatatttgtt
     9061 ggcaaaatgc taatcattag ttttatggaa aaaatagata aattagaccg taagattttg
     9121 cggattattt cccaaaatgc ccgtaaacct tttaaggatg ttgcggaaga atgtgaagtg
     9181 tccagggcag ctattcatca gcgggtgcaa cgtcttatag atatgggcgt aattaccggg
     9241 tcgggatatt ctgtaaatcc caaaatgctt ggttttcaga tttgtgttta cataggtata
     9301 acgctcgaaa gagcttcgat gtataaagac gtggtagctg aactggaaaa aattccagaa
     9361 gtcgtagaat cgcaatatac acttggagct tatactatcc tgataaaaat gtatgccaaa
     9421 aatgatgaac atctgatgga actgctcaac ggcaaacttc aggaaatacc cggtgtggct
     9481 actactgaaa cccttacttc gctggaccag cgaattaaac gtacgatccc gatagaataa
     9541 aagaattaaa tcaataatac taaagccgct tatcacacta agtggctttt tgcattaaaa
     9601 tgattaactt tgcacacaga attaggaaag aagaattcat taccatattt gcctgaaaaa
     9661 acctatcaaa ctacaaaaga ataaaataga aggcagaaaa ttttacaaaa tgaaaacagt
     9721 tttaagcgga atccgtccca ccggaaattt acatttggga aattattttg gagctttaag
     9781 caattttgtc agcatgcaga atgagtacaa ctgctttttc ttcatagcag attatcattc
     9841 acttacaaca cacccaactc cccagaacat tcagggaaat gtaaaaagtg tacttgcgca
     9901 atacctggct gcaggtcttg atccggaaaa ggccacgtta tatatccaaa gtgatgttcc
     9961 tgaagtaatt gagctctatt tatttctcaa tatgaatgct tatgtcgggg aactggaacg
    10021 ttgcacttca ttcaaagata aaatccgtca acagccccag aatgtaaatg caggacttct
    10081 gacttatccg acacttatgg ctgccgacat acttatacac aaagcagatt ttgtccctgt
    10141 gggaaaagat caggaacaac acctcgaaat ggcacgtacg tttggaaatc gtttcaacag
    10201 gatgtacaat catgagtatt tccccgaacc tcaggcattt aatttcggta aagaactggt
    10261 gaaaatcccg gcactcaacg ggtccggaaa aatgggaaaa tcagaaggag aggacagcac
    10321 cattttcctc accgatacac ctgaagtgat cctcaaaaag gtgaaacgtg cagttactga
    10381 tgccggacct acggaaccca atcagcctgt ttcagaacct gttgccaatc tgttccagtt
    10441 gatgcatgta gtttcaaaac ccgaaacgct tgaatttttc cgcgaacagt acagcacatg
    10501 tcagattcgg tatggtgatc tgaaaaaaca gctggctgaa gatatgatcg cttttgtcaa
    10561 tcctataagg gaacgaatac tggaaattgc aagtgatgaa atttatctga ataaggtagc
    10621 gcagatagga aaagaaaaag cacaggaaag tgcccgcaaa accatacggg aagtgcgaga
    10681 aattatcggt ttcaaacctt tttgataaga tacccgggtt gtgatggcaa aacttatctc
    10741 ttagtatatt ttagtatgta aaaatcgaac tattttgtgg aaaagtttac ttcaagtcac
    10801 agattttcga cagagtaaca gaaatttgaa taataaaaaa ttaaaattga caaaatagca
    10861 aaccgaagaa aagatttcat tgaaaagcaa gaatttgaat cttgaaagag ttcgcaaata
    10921 gagactctca aaaaatagct taaaacagcc aaaaagaatt gctaactcaa tgatttttcg
    10981 tagatttgca tccccaaaac cacactatta acgctgaatg gaaaataaaa aaatcaaacg
    11041 ggcattaatt tcagtttatc ataaagataa attagatgaa attattaaga aactgtatgc
    11101 tgaaggggta agttttgtat ctaccggagg aacaaaatcg tttattgaat cgcttggaat
    11161 tccttgcgat accgttgaag atctgaccag ctatccgtct attctaggtg gaagagtgaa
    11221 aacccttcat ccgaaagtat ttggaggaat acttgcccgg cgcgacatgg agcaggatct
    11281 ggcacagttg gaacaattcg aaatcccgga gattgatctg gtaatcgttg atttatatcc
    11341 gttcgaagcc acaatagcag ccggagctga tgaagccact tccattgaaa aaattgatat
    11401 cggaggaata tcacttatac gtgctgctgc taaaaactat aaagatgtgg ttatacttgc
    11461 ctccgaagca caatatgttc cgtttttgga attaataaat cagaatggtg caagcaccac
    11521 actcgaagaa cgccggtggt ttgccaagga agcatttggt gtttcatcgc attacgattc
    11581 ggctatattc aactatttcg acggagacaa tgccagcgca ttccggatga gtgccaatgg
    11641 atcaaaatcc ctccgttatg gcgaaaatcc tcatcagagc ggttactttt tcgggcaatt
    11701 tgatgatatg tttgagcaac tgcatggtaa ggaaatatcc tacaataact tgcttgacat
    11761 tgatgcggct gtcagcctga taaatgattt tgaagatatt acatttgcca ttctgaaaca
    11821 taacaatgct tgtgggttag cctcacgtcc caacttaaaa gaagcctggg aagcagctct
    11881 tgccggtgat ccggtttcag cgtttggagg cgttcttatt accaatgcaa tcgttgataa
    11941 ggaaacggct gaagaaatca ataaattgtt cctggaagtc attatagctc cggattacga
    12001 tctggacgcc ctgcaaatac tcactcaaaa gaaaaacagg ataatccttg ttttgctcaa
    12061 taataaagtg ccgaaaaaac agtttcgttc agttctgaat ggagtactcg tacaggataa
    12121 ggatttacat acagaagctt caggagatct taaagtggta accgaaaaag aacctacggc
    12181 agaagaaatt gaggacatga tttttgcaaa tagaattgta aaacattcaa aatcaaatgc
    12241 aattgttctg gcaaaaggaa aacagctgat agctagtgga gtagggcaga cttcgcgtgt
    12301 agatgcactt aaacaggcta ttgaaaaagc caaaacgttc gagtttgacc tggaaggtgc
    12361 tgtgatggcc tcggatgctt tcttcccatt ttccgactgt gtggaaattg ctgctgaagc
    12421 aggaattaat gctgttattc agcccggagg ttctgttcgt gatgatgaaa gcatagaaat
    12481 gtgcaacaaa aaaggattgt cgatggttac taccggttac agacatttta aacattaaga
    12541 tatagctaaa aaaaaatata tgggactttt ttcatttacg caggaaatag ccattgactt
    12601 aggaactgct aatacgataa tcattcacga tgataaaata gtagtcgatg aaccctctgt
    12661 ggttgcatta gacaaacgta ccgataagtt gattgccatt ggcgagaaag cacgtcagat
    12721 gcagggaaaa gagaatgaag gaattcgcac tgttcgtcct ttacgcgacg gtgtaattgc
    12781 cgacttcttt gcagccgagc tgatgatacg cggtatgata aagatgatac cttcaaaaaa
    12841 tcatttgttt tctcccactc tcaggatggt ggtatgtatt ccttccggaa gtaccgaagt
    12901 tgaaatacgt gccgtacgtg actcttcgga gcatgccggc ggacgcgatg tttatatgat
    12961 ttacgaaccc atggcggctg ccatcggtat aggtatcgat gttgaagctc ctacaggttg
    13021 tatgattgtg gatattggag gaggtactac agaaattgct gttatttcgc tgggaggtat
    13081 agtttccaat aaatcaatcc gaatagccgg agatgattta acagcagata tcatggaata
    13141 tatgggacgt atgcacaata tcaaagtggg agaacgtaca gcagagatga ttaaaattaa
    13201 tgtgggtgcg gcattgaccg atcttgaaga tgctccggaa gattatattg tacgcggacc
    13261 aaaccgtatg actgcattac ctatggaagt gccggtttcc tatcaggaaa ttgcacactg
    13321 ccttgaaaaa tcaatatcga aaattgaagc agcagtgctg agcgctctgg aacaaacacc
    13381 accggaatta tatgcggata ttgttcagaa gggaatttac cttgccggtg gtggtgcttt
    13441 acttcgcgga ctggacaagc gtctgacaga taaactgaat atacaatttc acattgcaga
    13501 tgatcctttg cacgctgtag cgcggggtac cggaattgca ctgaaaaatg tagataaatt
    13561 cggtttcctg atcagataaa aagaagattt taagttcaga taattcagaa taacatacaa
    13621 acacacgatt tacatctcgt ttatcgtgtg ttttaaaatc ataatcagtc atttcgcctc
    13681 ttgaaaaatc taatcagttt tttgataaga tacagcgtag tttttctttt tatagcacta
    13741 gaaataattt cgctgatact gatttccaat aacaaaggat atcaaaggag cgtaatgctt
    13801 tcatcgggca atgccatggt gggcagaatg tatgaaatga ccaattcatt tgtcgagttt
    13861 ttcaagctga gtgaagcaaa tgataatctt gcaaacgaga atacacaact gagaaacagg
    13921 gttgtagaat tagaaaacaa actgagtaca ctcacagata ctcttaacag cgtaagctgg
    13981 aaaaatgttc ctgtttcacc tgaaaaggaa tattcataca ttacggcaaa agtcatcagg
    14041 aataccaccc atctttctca aaattacatt actctcaata agggttcgaa cgaaggaatt
    14101 accccggata tgggtgtggt cggtgaagaa ggtgtggtcg gaatagtaaa aagcgtgtca
    14161 cccaggttct cggtggtcat tcccatactt aatccgaaaa ttcagatcag cagtaaattt
    14221 aaaaagaatg attattccgg accactcgtc tggaatggta aagatgcccg ctttgccagt
    14281 ctgaacgata ttgcacgcca tgttcgtttt agtctgggag atacgctgat aaccagtgga
    14341 ctgacaaaaa catttcccga aggtatcatg attggaacaa tcaacgatta caacataaag
    14401 gaaagtgatg cttattataa tattcaggta aaacttgctg ttaactttcg gacactcact
    14461 catgtaaaga ttatcaagta taagaattac agagaacaaa gtgttctgga acaaacggct
    14521 gctgatgaag gcaaaaaaaa gtaaacgatg gtatcagcac ttcaaaacat attacgattt
    14581 atttttttga tattgattca ggtttttctg cttgacaata ttcagtttat gggttatatc
    14641 aatcccatga tatatgtttt gtttatctta ttgcttcctg tccgttttcc ccgctgggct
    14701 ttgttgattt ctgcatttgc gatgggtttg attatcgata ttttttctaa tacagccgga
    14761 atgcatgcgt tcgcactcgt atttgcagct tttcttcgtg atccggtaat taatattttt
    14821 acatccatcg atgaaggttc aaatccaacg ccttcatttc atacttttgg agtttcaccc
    14881 tttataaaat atgtgataac aattgttttg cttcatcata ttacactttt tttgcttgaa
    14941 tcattttcat ttcttaatct gtcgctaata attccaaaaa tactcatcag ttctgcaatt
    15001 acaattctgc tagtattagc tatccaatca tttaagtcga aataaatgat aaacgataaa
    15061 cttcgaaaca gagggacagt aatcactatc atcatggccg ttatagtgat tatttatatt
    15121 ttgcgccttt tttcgttaca gattatcgaa accaagtata aaagatatgc cgacagtaat
    15181 gcatttctga ataaaaccat ttttcctccg cgtggactga tttacgaccg gaatgattca
    15241 ctactcgtat ataacaaacc cgcatacgat atcgctttta ttaatcgtga aattcaggga
    15301 ctggatacag cgtcattttg cagtgatctg aatataacca ccgatttttt cattgaaaaa
    15361 atgaaagaaa tcaaagaccg cgataaaaat ccgggttatt cgtcgtttac tccgcaggta
    15421 ttcatgacac aactcgatat tcgcgatatt gcaaatatac agcaatccat gtacaaatat
    15481 ccgggttttt atattcagac ccgaaccctg cgcgaatatc gttataatgc cggtgctcat
    15541 gttttgggaa gtatcggtga ggtctcgcag aaaaaaatag atgaggatga ttactataac
    15601 cggggcgatt atgccggaag agatggcata gaatacacgt atgagaaaga cctgagaggt
    15661 gtgaaagggg tggaaattct gttgcgcgac gcacgtggaa gattaaaagg taaatacgaa
    15721 aacggagaaa aagatattgc tccgaaagct ggaaaaaatc tgaaacttac tctggatata
    15781 aagcttcaac tacttgccga gcaactgctt gcagggaaag ttggaagtgt ggttgctatt
    15841 gaaccaaaaa ccggagagat cctggcaatg gtctcaaatc caacttttaa tccctcgctt
    15901 ttagtgggtc gtgagcgctc taaaaattat aatcttcttg ttaatgatcc aacaaaacct
    15961 ttgatgaacc gggcaacgca ggctcagtat tctccgggtt ccacatttaa gccttttcag
    16021 gcgcttttcg ggctacagtt aggcggcatc accgaaggta cgcgatttac atgcaacgga
    16081 ccctcttcct ggccaataaa gtgtactcac catcatggtt caccggtttc gttgctcaat
    16141 gccattgaac aaagctgtaa tccctatttc tggaatactt tcaggtcgat actggagaaa
    16201 gacggctatg gaaacaagaa tgtaaatttc aagaagagat tcaataaatg gcgggaaacg
    16261 gtaatggaat ttggtttcgg aaagaaattt gaagatacgg atatctatca gcagagcagg
    16321 ggaaatatac cttcggagaa attttatgat aaatatttcg gtaaaaccgg atggcgggct
    16381 aatacaatac gttcgctatc catcgggcag ggtgagattc tggtaactcc cttgcagtta
    16441 gccaacggaa tatctgccat tgccaataaa gggtattaca taactcctca cctgaacaaa
    16501 tcagattcaa tgctgacgca caaacacaaa acttccatcg aagaaaaata tttcagagtt
    16561 gtggatgaag gaatgtggcg ggtgaatgaa tttggtaccg gcagggctta taaaataccc
    16621 ggtgtatcaa gctgtggtaa aacaggtact gttcagaata acagggggaa agaccacgca
    16681 ctatttgttg gttatgctcc cagagagaat ccaaaaatcg ttcttgctgt ggtagttgaa
    16741 aattcgggct atggggcaac ctgggctgcg ccaattgcca gcctactgat ggaacagttt
    16801 atattacaca aaatagacag aactgaacta ttaaacagaa tatcaacatc tagaacaaac
    16861 gtaaatgtca cggactatta gcatgaaata cgcgttggac tggacaacgg tgggtatcta
    16921 cctgtttctg gtcttttttg gctggctgaa tgtttatggc gcaagctatg atttcgatca
    16981 gtcgagcata tttgatttta gtcagcgtgc cggaaagcag tttgtatgga tactgacagc
    17041 tttcgggttg ggtggtgtca ttctgctgat tgacagccgg cttttcaata tcttttcata
    17101 cgtattttat gtagcgatta tattgctact aattgttacc atttttgttg cttcagacgt
    17161 gaaagggtcg cgttcctggc tcgagttcgg tcccatcagt tttcagcctg cagagcttgc
    17221 caaaacagcc actgcgctgg ctttggcaaa gtttatgagt tcctacaatt acaaactgaa
    17281 aacctggaaa gaccttatcc cgttgggaat aattgttttt attccttttg ctctgattat
    17341 tcttcagaag gaaaccggaa gtgctctcgt gtttctggct tttctgctta tgttttatcg
    17401 ggagggaatg aacggaatat tcctgctttt gggcgttttg gcaatagtgt tgtttgttgt
    17461 ggcaataggt ttcagccaga caattattca aactgacagg ggaacgttgg ggatggtgct
    17521 ggcaatgtcg attatccttt taattcagtt ttgttttgct tttttcttta acaggcaccg
    17581 gaaagaagca ctaatgctac ttctgggtgt tgtcggtatt tcagcagcat cgtatgtatt
    17641 gaatatctgg tttaaaatta attatgatta cattgctatc ggaactgtcg ttgcttcgtg
    17701 tgtgtattgg attatcattg agatgtttaa ccgtcataga aaatactggc tactggcatt
    17761 gatttctttt ggttcattaa tattttgtta ttcagctgat tatattttta atgatgttct
    17821 ggagccacat cagcaggtac gaataaaagt gctgcttaat atggatgatg atctgaccgg
    17881 tgcgggttat aatgttaacc agtcgaagat agccattggt tcaggtggtt ttttgggcaa
    17941 aggttatctg aacggaactc agacaaaact gaaatatgta ccggaacagg ataccgattt
    18001 tattttctgt actgtaggcg aagaatgggg atttataggg tcggcagctg tactgctggt
    18061 ttatctctgg ctgctgctac ggattattca gattgccgaa cggcagcgcg atccgtttac
    18121 ccggatttac gcatattgtg ttgcaagtat ctttttcttt catctcacta tcaacgtagg
    18181 gatggtgctg gggattatgc ccgtaatcgg cattccattg ccatttctta gttatggggg
    18241 ttcatcgctc tggagtttta caatactctt gtttattctt ctcaggctcg acgcagcacg
    18301 gcgcataggc agttaagaat gccggttaaa tgctgtttat gtttattcct gttattttct
    18361 tatacagatc cagtgcatat acatccgtca ttcccgaaat ataatccaga acagccagaa
    18421 ctttgccgta tgtagtcgga ttgttgatat cgtattgttg cggtattcgg taaatcagtt
    18481 gtctggagta tgctttttcg ggctgcagaa cagcatttac aaatagatcc attagtgtca
    18541 gaattatctt ataacctgct agttctatat ccaacacctc ttgcgaccga taaattttat
    18601 cttttgcaat ttctgagcaa tgattatacg ctttcccggt ttgacccgac atatgtttaa
    18661 tcagcgaacc ctcgaatgtg cctgacagga tatctttttc attttcaata aaaatttctg
    18721 aacattcgtc aatcagttgt ccgatgaccg aagaacgaag gtatgcaatc tgttcattta
    18781 catcgctcac catttttagc gtttctaccc gttttttacg tttctcatcg tcgaaatatc
    18841 caagaaacag ttcctttgtt tcgtcagtag tgagtatttt cagtttatgc gcatcttcaa
    18901 tatccataat ctggtagcag atatcgtcag cagcttcgac cagatatacc aatggataac
    18961 ggttaaaacg gtttggatta tcagcttttc gggtaatgcc cagttgattg gctatttttt
    19021 caaaatcgat tctttctgac tcaaagaaac cgaatttttg ttttttcttg tcggcgtaaa
    19081 cagatgagta ggggtattta actatcgaag ccagcgtgct gtaggtaaga acaaacccac
    19141 ccggacgccg cccgttgaac tgatgcgtga gtagcctgaa agcgttggca tttccctcga
    19201 aatgaataca gtcattccac tccacttccg tcatttcatt tttcagaaac tgcccctttc
    19261 cttcggagaa aaaggtggcg atggcttctt cgcccgaatg tccgaatggt ggatttccca
    19321 gatcgtgagc cagacatgct gccgacacga taaaaccaat ttgatcgata tattccgaac
    19381 cggctgacga tagcgaacga gatacattca ttcccagtga ccgaccaaca ctggctactt
    19441 ccaggctgtg ggtaagccgg ttgtgaacaa aaatgctgcc cggtaaggga aacacctgag
    19501 ttttattttg cagccgccga aagggagcag aaaagatcaa ccggtcatag tcccgttgaa
    19561 attcattccg gttatcacga ttatggtcat gatagttttc catacccaga cgtacgggcg
    19621 aaagtaattg gttccagttc atttgtcagt atttttataa ttcaaacaaa gtatcaatct
    19681 tctgtataat attaagaata tcagatatct tctatttcaa tgtcatatgt gtctaacaga
    19741 ccggttactg ccggcaatga aaattttgct tttttaatcc ggttattttc aggattgatg
    19801 ctgtagttat atgatgttcg aaaatcggtt ttttcgagat ttgcatgatt gaaaacagca
    19861 agtgaaaaat cacagtcgtt gaataccgtg ccggtgaaat cgctttcggt aaagtcggtt
    19921 tcatgcagca aacaatgtgc aaaaactgca tttttaaatt tgagttgata aaatgacgaa
    19981 tggtttaact gacaattttc agcagtgaac gaaagtccaa accggttgca gttttcaaaa
    20041 ttcaatccca gcattttaca ttccctgaat ttagtttcac gtatcgcagt atcggtcaaa
    20101 acagccatac tcaggttaca gccagtaaaa agacactcag agaatatgat attagaaagg
    20161 tcggcagaat taaaattaca attcacaaac gtgcaatttt catattcccc ttttttcaaa
    20221 gggatttgat tgaaatcaat cttctcaaat attttattta ccgcatattc gttttccatt
    20281 tcagatgttg ctgttttgtg gatttatagc aattttcttt tttgccaaac ctgatacgaa
    20341 ttaaaaatat tctgccacag aacgtatgca cccggagcaa tggatgaaat cggattgaga
    20401 aacatttgtg ccatccagat agcaagtatc gtgtttttct gcccaagact ttgaccccct
    20461 gcaatggtat cgttatattt tttcccgatt tttttacctg tgacgaactg tccgatacag
    20521 gcaaccaaag cgcctgcagc taccagaatt tcggtagtat aattattgct gttttgttgc
    20581 aggataaatt ccacggtttt agcagtaact attatcaatg aaaaactcca gatataaaac
    20641 gatagaccgg agaaggatcc tatttttgca gttgtttcag gtgagatttt tttcagaaca
    20701 atagccagaa caaatggtgt gagcagaaga acaaataccc gacgcgagat agccagaaat
    20761 gaatcaaaaa aaggcaatgt ttcataactc ccgagcagtg aaaaaataag cggagcagcg
    20821 atggctacgc tcagattact caataaacta tatgctgtca ggctttccac attccctttc
    20881 agcatgccgg taacaaccgg agcagcggtt ccggtgggag ccagcataca aatcatcaca
    20941 ccttgtgcaa gtaccagatt ccacggactg aggatatagt aaacaataac acaacccaaa
    21001 atctggatta aaatcagcca cagatgaagt ccggagagct gcagatgctt gaaattcagt
    21061 ttgcagtatg ttagaaaaag cattgtgaaa attaggtacg gagtaagaaa agccagtgag
    21121 ccaaagaatt ggtgaaaaac tgcgcccgtt gtcattgcaa ggggcatcat atagggtttt
    21181 atttttttga acataaagag tgcacgcgtt tttttgttgt aatattcgga aataatcaat
    21241 aattaatcgg ctgcagttat tttggcatgg aatattgctt catttcattt ctcagttttt
    21301 tactgcttcc attggtgacc aggtccatct ggtgccagaa attctcgctg tggttcattt
    21361 cccgggtgtg acaaagttca tgcagaatga cataatcaat caaatggaat ggcagaagca
    21421 taaggtaaaa cgacaggttg atattttttc tggtattaca acttccccag cgcgttttag
    21481 aagactgaat tttcacatcg gtaaatgaga agccgtattg atctgctaat tcacgggtac
    21541 gttgaggtaa aatccgttta gcttccattc gtaggaaata gttgatgctg ttccagaaat
    21601 aggtttgtgt ctgcatttct tccggattca gttgttgcgg atactctatt gtcagcacac
    21661 ccgatttcag tgaagagaaa atattgttgc gttctgcttt tctgatacaa acatcaaaag
    21721 tgcatgtttt cagtatattt gattcggaaa tgacaactgt cttttgattg actcccgatt
    21781 gtttttttat taattttggt ctcactgaat caataaattt cagagcgtct gtttcactgc
    21841 ttccaggggg taatgttacg accagtgagt tgctgagtat gcgcacagaa atccgggtag
    21901 tccgtgtact gcgtctgaag gtgatggtgc cgagatcttt atctgaaatc atgggatgaa
    21961 aactgcctga tatttcaagc aaagttacaa tatttttttg tgaatggtgt gaaaatgagc
    22021 ggaaacggct aactttgcag gaatttatga caaagactgc aaatggatag ttggacacaa
    22081 aggacagaat tgcttacagg caataacgga ttgaataaac taagaaattc ttctgttcta
    22141 atcgttggtt tgggaggggt tggtggtcta gctgccgaaa tgatttgccg tgccggagtt
    22201 gggcggatga ccctgattga ccgcgatacg gtgacctcaa ccaatatcaa ccggcaaatt
    22261 gctgcattgc attcaacagt cggacaactc aaaacagaga ttatcgcttc caggctgaag
    22321 gatatcaatc cggttctgga actgactgta atatcggact ggctgaacga agaaaataca
    22381 gataaaattc tcgatgaagg taaatttgac tttgtggtag atgctatcga tactctttca
    22441 cccaaggtat ttctgataaa atcgtgtacc gagaaaggca tccggatcat atcgtccatg
    22501 ggttcgggag caaagatgga tccttcgaaa gtgcagattt ccgatatttc gaaaacaaat
    22561 tactgcccgc tggcaaaagc cgtaaggcaa cgattatcga agttgggtat aaaaaaagga
    22621 cttactgtcg tttattcgac cgaagaatcc gacagaaaca gcgtcatcct taccgaagga
    22681 gaactttaca aaaagtcaac taccggaacg atttcatata tgccggcttt gttcggactt
    22741 tatctgtctt cgtacgtcat cagagaaata ataaaaaact gagaaatatg ataactgcaa
    22801 aaaacataca taaaagttat ggcgatctgg aagtcttgag aggggtggat ttacatgtcg
    22861 ataaaggaga aatcgtatcg attatgggac cgagcggagc cggtaaaacc accttgcttc
    22921 agatactcgg aactctcgac aaacccaatg ccggtgaagt ggaagtggat ggaattaatt
    22981 ttagtaaatt gaattccgat cagttatctg tattccgaaa cagacacatt ggctttattt
    23041 ttcagtttca ccagctttta cccgagttta ctgccattga gaacgtgctt atacctgcta
    23101 tgataggaaa aatgaatata aaacaggcca gagaaaaagc aacagaattg cttcatttcc
    23161 tgcagctcgg cgaccggctt caccacaaac cctccgaact ttcgggtggt gaaaaacaac
    23221 gggtggcagt agccagggcg ttaatcaaca atccgtcact catccttgcc gacgaacctt
    23281 cggggagtct cgatacaaaa aacaaggacg aacttcataa attactgttt gagttaagag
    23341 ataaatataa cctcacagaa gtgattgtaa ctcacgataa ggaactggca aaactatgtg
    23401 accgtacaat aggaatgaag gatggactaa ttcaactgac aattgacaag taaagatagt
    23461 accctcgccg ggaatcgaac ccgaatttaa agtttaggaa acttccgttc tatccattga
    23521 actacaaggg caaactgagc gacaaaatta catctttttg ttgaataata aaaacgtcgg
    23581 tcttcggtac ttcagaaaaa aaatataatg agcaagaaac aaaaatacta tacggtttgg
    23641 gaaggtgtta atcccggtgt ttacaattcg tggaaagaat gtgagaaaca aatcaaaggt
    23701 tacgaaggag ctaaatttaa gtctttcgaa accataaagg aagctcagga tgcttttatg
    23761 tcttcttgct gggagtatat cgggaaaaat gcagtcaata caaaaccgac acccaactac
    23821 aggaatttac cgtcatcaga gcagccgaaa ctgaacagta ttgctgttga tgccgcatgc
    23881 tcgggtaatc ctggaaaaat ggaatatcgg ggtgtggatg tgaataccgg aatagagata
    23941 ttccgacaag gtccattcga gaatggtaca aacaatatcg gagagttttt ggcaattgtt
    24001 catgctcttg cactttttaa taaaactaat ccacacatcc ccgtttatac cgatagtaaa
    24061 acggctcttg cctgggtaaa agctaaaaaa tccaaaacaa aactaggaat ggatgaacaa
    24121 aatcagtata tttttcaact gattgaacgg gctgaaaaat ggctcaggga gaataagttc
    24181 aacaatccac tcataaaatg ggaaactgaa aaatggggcg aaattccggc agattttggc
    24241 cgaaaataat tcacagtatg aatagaataa agaaatgttg tttattatca cttctcagtc
    24301 tgtttgcttt gttgcaggca ggttgctcgg ataatattac accggatcag ccattggtcg
    24361 taatcattaa agccgatgat tttaatgact attcccccaa ctggcaacgt tttattgcaa
    24421 ttctgcagca aaatgatata tcggctgctg ccggagtgac atccacactc atttcatcca
    24481 atcattctgc cattataaaa atcagggaac tttcgggact cagacgctcc aacaaccaac
    24541 cactgattga attttggaat cacggttatg atcatgcagg ggacaatggg gattctcccg
    24601 agttcgataa ctctgatgtt gatctccaga agaaacattt acagttagca cagcgttttt
    24661 tcagcgattc gcttcatatc atagccaaaa cattcagcgc cccgcataac agatcaacta
    24721 cagctaccct acatgcatta tcctgtatac ccgaactgaa tgtgatcatg aggtacaaaa
    24781 atcacgaaaa attttccctg aaaagaccct ggattgatcc caataaaaag aaatttaaac
    24841 ccggtgataa gatttgtctg aatatcaagt ttcagtatct tcttgatatt ccatcgacgg
    24901 atgttgagaa ttatttcaaa gccggggaaa aggataacta tctgctaatt cagcttcatc
    24961 cgaatccctg ggatgaaaat gatttcaatg attttcagaa aatgatttct tttctgaaag
    25021 ggaaaaatgt aaaatttatg acaccggatg agtactttca gtacctggat gtgatgaatt
    25081 aaaaaatcag acaaccgatg aaattatcgt ttaaacataa ttacttttct ctctttttgc
    25141 tccttttcac aactctggca atggttgcag tgcataggtt gtacagtaca caggtatatg
    25201 taacaaagga aaatgtcagt ttgtttggaa atattatctt gcttatattt gcgttgtcat
    25261 cggtcctgat ttttatatct caaatcatcc ttgtccgaag aattctgaga ccttttcttt
    25321 ctgtagaaaa tttcgtatct ctttcccttg tcctgttact ggtattatcg gtttttcaga
    25381 aggaagtggg tatttacgcc accggatttt ttgtgttgtt tgctctcgtg tctttttttg
    25441 tgagggggaa aatatacacc ctcaataaag tgtattattt catattcctg tatgctgttt
    25501 ttctcatggc agggactatt tacacgcccc gcggttttca tttccccgaa atgacctata
    25561 ctttttattt actgccgttg tcattcagtc tattccggct caataagcaa accctcctgt
    25621 tgattctgaa aattgtattc cgcatcatgg ctgtttatat ggctgtttcg gttgtttact
    25681 ggtggttcaa tttgctggtt ttgaataagt ctgctttgga atggattact atgaaatttc
    25741 ctaacgcata tgactacgtc ggtacctggg gacattattc tcacccaagt tatatctcat
    25801 tggtattatt ctctgctttg atttcgggtt tttatctcaa tttcaaaatg gacaatactg
    25861 taaccctttc caagttcgag ctgataacat acattggttt atgtctgggt tttgtattag
    25921 tgacggaaag tcgtatcggg ttggttgagt taatcataat cgttttgatc agtctgcttt
    25981 actatatcag actgaaaact ccccatttca aactggctct ggtggttgca gtgttagttg
    26041 gggtggttat tctcatggct ggcgaaaaca aagtgagtcg gtttatccgg gatgatgttc
    26101 gtaaagcgga ttatacgctg gcagtcaatt atataaaaga gcatcctgtt atcggtgcag
    26161 gctatcatca gcaacgagca gcgttggaat accaggataa gaagatgtcg gatgtaacaa
    26221 ggcctgaggg tgctccgccc attacttaca cccacaatca atttctgggc gatatggttc
    26281 agtttggtat tcccgggctt atttgtctgc tgatattgct taccggatta atctggaacg
    26341 cgcttcggtc acggagttat cttttacagc tatttatgct gatttacctg ttttttatgt
    26401 taattgaaga gcctttatat gctcaggaag gtattacacg gtttatgtct tatttatgtt
    26461 tgtttgtaca catcagcgag tgcgaaaaac cattgaaagc cttcaactta aggaatttgt
    26521 tttcgaaaag taagacgacg taactgattg tattttttct tatacttctc ataatcacgc
    26581 agaaactgta tagggttgtc tatcaatcgg tcataaactt ccttatggtt ggcgtagatt
    26641 tgcatctgtg ttgccgcaat tttatcctga tgattgtcgg taaacgaatc ggtatgtttc
    26701 cggtagaaaa aatattcctt atcaataaaa tacacttcac cgcctttttc gagcagactt
    26761 atccagaggt cccagtcttc gctggtaatg atatttggat cataaccgcc agcgctgtca
    26821 aaatctaccc gccggaagac ggctgtattg tgaatacagt ttttcagtaa tagctgtctg
    26881 aaattataag ctggtaattt ccatttccct tttttatctc cgaaaaatac tccgctgcag
    26941 taaacgagtt tcaccgaagt attattctcg agtatttcta cggcttcagc aatatattcg
    27001 ggcgctatca ggtcgtcgga atccagcgga agaatatatt taccggaaga atgagccact
    27061 gcattgtttt tggttaccgg aatgggtgat tttggctggt ggaagtattt tatgcggctg
    27121 tcggtccggg ccatttcctt gattatcatt gccgagttat cggtcgaatc atcgtccatc
    27181 acgatgcatt cccagttttt atagttgcat ttctgaagac actccagtgt ctcccgaata
    27241 tatttctccc ggttgtggat gggcgtgatt accgatattt taggttctga gtgcataaga
    27301 agttttttaa atttttattc aatcctgtac gattgttttt tagcgagata cctattgctg
    27361 aatatatttt ttatagttcg taagtcaaag atactcattt agcgatgata gatacctgca
    27421 ttctttattt tatttacagg cagatttttg gaaatatgtt ttcgatattt ataaacaatc
    27481 ggatgaaata tatttccaaa tacagtccat gggagtgggt aataaggatt tctacctgaa
    27541 tacaactgct ttagccgaat aatcacagca tgaaatatgt tttttggttg aataaatttt
    27601 tcacggggat gctttgccgg atgttttttc aatgattcca tattagtatc cagcagcgat
    27661 aaaaaagccg tatatatttc ggcagtcatc ggggtatttc cttttgctat ccaggccggt
    27721 acagctacca actctttcca gtaatcgggc atatatctgt tgtgctcatc gtacaccctg
    27781 gctgctcctt tctggttttc gggccggcca attaaataaa tatccggatt ttcgaattca
    27841 taccagcagt ttttgaaaga cacctctgta gcttttatat catgccatgc tccgccataa
    27901 aagtgcatca gatatgctct tatataatcc gaacgatgaa cgacgctgag atatggaaag
    27961 gcttcatgta acggatgctc aggtttaatg aatgcagcaa gattttcttt tgaaatcaaa
    28021 caaataggaa cctgaatatt cttttgtaaa tactcataac tcaacagcct gttagcattc
    28081 ataggagcac cttcccagta acaccagacc acaaatggaa cattatgctg ataagtaagt
    28141 tccgaaggat tatttatata ttccatagtt tgaatttact atatatctaa gttcatgtac
    28201 tgaaatatca aaggtataat cttgtgaatg gtttatagac caactgcaga aaaaaaaatt
    28261 ttcgattgat gtgtcgatat tttattttta atttttcgga tttaatctta tcgatataaa
    28321 acgtgattgt cgattttatt tctttagcag agaagcctct ttttatcatt ttaataaata
    28381 aatagttgat catcttctct tcagatttca gaatttcttc ggctgtatat ttgtttttat
    28441 catagattgg taaaaacttc tcccatttac caaacacttg tattaagttc tcaaaatatc
    28501 ttttatttct aaatcctttg gaataatgct ctattaaaat atcaaaaacc acataattct
    28561 gaaatcctgc aaaagcagat tgaagacaaa tatctaaatc ataaccgtga aaacctttca
    28621 gcttttcatc gaaggttatt tttgagaata tattgcggtt tgcacccaaa aacacaccat
    28681 ccagcagaat tacttcctgt ttatcatcat tatgctctaa aggaagtcgc acatgctctg
    28741 agtgtttttc tgactgaatt atatttatat atttttcctg agatgacgac catgatgaag
    28801 gaacagagag tacggtgtta cttcctgcta ctccaattag tcctgtagtt gaattattta
    28861 aatgtgattt aataatattt ccccagtttt ttgtttgaaa attcacatct tcatgcacaa
    28921 aacataaaaa gggatattta ctttcggcaa ttcctttgtt gtatgctgaa aaaatggaat
    28981 attcattgcg cgaattatta atacatacaa tttcatacgg aactccaatc gtattattta
    29041 tattttgtct tacagattcc gacaattttt cgtgtttcga acaaataata acagaaatca
    29101 tggagttgtt tttaatgttt atctaatccg tttttacaaa caaattcatt tatctcttgt
    29161 ttttgcctgc acaaattcaa attaaagtcc tgattatatc cttcaggatg actcaaatga
    29221 tattgtaccg ccagattacg cattgatacg agctgaaatc cggctgcttt caagcgccac
    29281 gccagatcgt agtcctcacc aactgcaggc aacttatagt cttcatcaaa tccgttgatg
    29341 gcatagattg cttctttgct gaatgacata ttgcatccga caagtttatc atttttgcgg
    29401 tttcggggaa taattctcaa tatactttca ggagaaatga atattccttc ttcaataaat
    29461 tttatttttc ctttaccgaa aaacattttt cggagtagta tttgctgcaa attcctgata
    29521 ttatcactgt tgtcgagtat gtattctgtc atgtaatcgt ccagttttac acgtttgccg
    29581 gccagtattt tgtttacttc ggcgtgatgc aaatgcatct caataaagcg gggatgcagt
    29641 acacaatcgc catcgataaa aataagccag tcagcttccg aggctctgat ggcactgttc
    29701 aaacagtgat tttttcgcca gccttcgtcc tttcttgtga tgtgcttata aggcatctca
    29761 aacgggtacc cggcaatgaa ctctttcatt tccgggcttt ctccatcctc tgaaatgatg
    29821 gtctcgaagt ctttctcggt ctgaaaatga agcgaatcga gtaccggttt cagaaaccga
    29881 atatttttat aaacagatat tattaatgat gctttcattc gttatttttc gagaattatc
    29941 aagaaacttt tcagggtaca acctgatatt tgctgctcgt aatatcccag acagaattta
    30001 tagctgtcag atattgtttg ccggtacatg tcgcgggctt tggatgtttt aattccgttg
    30061 ccgggaatgt gatatcgttt ttcggtaaat tcggtaatct ttattttctc tgcaactgat
    30121 ggaaacagct tcgtaatctg attattcctg attgatattt cttcattcga aaggtaaacg
    30181 gcattttcat aaacagagta ttccggattg acaaaaatat tctcgggcag aaaaaaatgc
    30241 cggatgatgt tcaattttcg ggaatgagtg cgctgataat gcaattttcc tttcagttcg
    30301 cggtagaaaa aagctttatg gcggatatta agcatatcaa taagttcctt cttaagattc
    30361 cgggttttaa ctcgttttga tccgcttcga attctgtaat taagtccatt gatgggtaat
    30421 cgtacaaatt caccgcctgt tttgaacaac gaaatccaga aatcccagtc ttctcttccc
    30481 gttatttctt cgcaataacc acctgttttg tcccaatcag cttttcggta catggagcaa
    30541 acatcaatta aatttcttct tgctaaaagg ttgtagctga atggtggaaa attccatcgt
    30601 cctgtttttt caccaaaaaa agaggcctcc cggctaacaa cttttacttc gggtttattt
    30661 tccagcactt ttacggcttc ttcgatgtaa ttgtttgaaa tagtatcatc cgaatcaaca
    30721 ggtaggatat atttcccgtg cgataatccg atcgcatgat ttctggcaac tgatgcacca
    30781 gcgttaggtt gtacaaagca acggatgcga gcgtcttgca gggcatgatt ttgagcaatt
    30841 tccatggatt tatctgtgga accatcgtcc atgatgatca cctcaaaatt cggataagtc
    30901 gatattaata cagattcgag ggtttcagcc aggtataatt cggaattgta aaccggtata
    30961 atgaccgaaa ccagtggaaa attttccgat tctgatgcct tatgtatgtc gtttgcaggt
    31021 tttatattta ccatttcaga ttttgtctaa tcctttcgtg tacttttgcc agtaatattt
    31081 aagaggattg gtatatttca gttttttcca ggggcggctg ctgtgaggaa ggtgcagtac
    31141 aggcgcttcg gtcaataaat agtttctgaa acccattgaa gtggatttgt gggaaataaa
    31201 gatatcgtac caggtctttt gtgcatccag atttgtaaaa tcatacgcgt tcagcaattg
    31261 gttggaaagc agggtacagc aaaaactcag tcgttttttg gtttcaacag taccttctct
    31321 gaagttctga gcaaatagat acggaaaatt aatttttccg gattcatccg ttgttacagc
    31381 agctataagc ccagggtttt tcaattttcc ggattggagt atcaactgcc ggatggtgtc
    31441 gtttttaatc accacatccg attcaacaat cagcaaatgc gcgttgtcgt ctatcgcttt
    31501 tttctgagcc aattgcaaca caagccgata attgggcgat ggatgcgaag ttatatcttt
    31561 cagattgact aattcaaatc caaatcgtga agatgcttcc tgcaaaagga aagtagtttc
    31621 gtcgtcgctg aaatcattgt aaatcgtgta tttcaggtcg gaataacctt cagaaccgac
    31681 aatgttttca atcgtcctga tggtagtccc gaatgaattt tttaccggtg ttatgatatg
    31741 tatgttcatg cttgtttttt caaaaacctg ttcagtacag gtatatcttt gagaggtaaa
    31801 tctcttttaa taaagaaaac gataaaaaca gccataagca cagttttcag agctaaattt
    31861 acgatcaaat aacgtgtttc gataaaagta cttatgccaa acaacactag agttagtgca
    31921 aaataaattc caagtttctt taagttataa tttatcggca taaatttctg tccaaagaaa
    31981 tatgacaaga gcatcacaat aaaatatcct gcaaatgcag accatgccga gccccaataa
    32041 ctgtatttgg gtacaaaaat aatattcccg acaaatacaa ttaacgttcc gatagcagca
    32101 aaccacgctc cataatgatt tttatctttc aatttatacc aaatagaaag attaaaatat
    32161 attccctgaa aaataaacga aaacagaata attggaacaa cttttaatcc cacccaatat
    32221 tcacttggta caagatattt tattatgtcc atatataaaa tcattcccaa aaaaattaaa
    32281 aatgaaagta taacgaagta tttcattgct tcactgtatg tttgtgaact gttctcatct
    32341 ttgtgtttag caaatacaaa gggttcataa gcaaatcgaa aagcctgagt aaacatcatt
    32401 ataaccaacg ccaacttact gactgctcca taaataccca aatcgtttcc tccttttgat
    32461 cctacttgta gaaaaggaaa tatcattttg tctaaattct gattggcaat acctgctata
    32521 cccaaaaaca acaagggata ggaatatttg agcatttgct ttaatagttg actatcaaac
    32581 ttaaattcag cttcaaaaat atatctggat aatatcagcg tttgaagcgt tgttgaaata
    32641 atatttgaaa taaaaatata acctactcca taatcaggac gatagaacca ggaaattgta
    32701 gaaggagctg ctttcatcaa aataggacaa agtaccaaaa agaatacatt caacactaca
    32761 ttcagaccaa catataacaa cttcataccc gcaaacacat acggacgttt aataaaacgc
    32821 agataaacga atggaataca cgcaaaagca tccatcgcca cacttaatcc aagcatccag
    32881 atatattcag gatgatttct gtagcccaaa gtatttgcta taggttgttg aaatacaata
    32941 atgataattg caaaaagcag acttgtaaac cctatggaaa tcagtgcatt accatacact
    33001 tttttagcat ttgccgtatc tttatttgca aaacggaaaa aaccagtttc cattccataa
    33061 gttaaagcaa caattaatat agaagtccat gcatataaat gggtaacaac accatattct
    33121 gcaggatttg gtagcgtgta agtatatagc accacaagca tccaactcac aaattttgca
    33181 gcaatattag taccgccata aattacggta tcctttgcca gcgatttcat ttcttttgcc
    33241 ataaattgat cggctctttt gtgtatttaa gtatgttcaa actacttttt tctgaatttg
    33301 cccagaattt tgtcaacgaa ttttaattca ggtgttggtt cgaaatcttc cggtttcagg
    33361 tatccgcctt caattaagag acgaatagct tcttcggcct gtgaggcacg tacatcgagt
    33421 tttacacccc cgttcacatt ggcaagatag gcgcggttga tgatctcgtc tctcccgaaa
    33481 cattcaatac ccatcgattc aagataggct tttaccattt caaaatcagc agactgactg
    33541 aaagttctga tgttaaccag ttcatccatt ttgttggttt tattaaaatt aaaataatgt
    33601 tcccatatct cccccgaatt tcactttacc caggtgcttg taagccagtt cggtaacttc
    33661 gcgtccgcga ggagtgcgtt tcagaaaacc ttctttaata aggaaaggtt catagacttc
    33721 ttcaagtgtg ccggggtctt cgctgagtgc agtggcaatc gtggtaagtc cgacaggtcc
    33781 gccctgaaat ttatcaataa tggtagtgag tatcttatta tcaatttcgt ccagtccgta
    33841 cttgtcaata tttagtgctt cgagtgcata atcggctatt tcaggattaa tcgttccgtt
    33901 tccttttacc tgtgcgaaat cccgtacccg tctgagcaac gcattggcaa tacggggtgt
    33961 tccacggctt cgtccggcaa tttcctgagc agctttgtga tcaatctgca cgttcagcaa
    34021 tcttgccgag cgttcgatga tgccggtgat gacctgtgta tcgtagtatt caaaatgaca
    34081 gttgatgcca aaccgcgccc gtaacggact ggtcagaagc ccgctgcgtg ttgtcgcgcc
    34141 aatgagtgta aacggattca gctccagctg aatggatcgt gcactcggac ctttatctat
    34201 catgatatcg atacggtaat cctccattgc agagtagagg tattcctcca cgatgggact
    34261 taatctgtga atttcgtcaa taaaaagaac atcatttgac tccagactgg tgagtagtcc
    34321 tgccagatca cccggtttgt cgagcaccgg tcccgatgtg attttaaatc ccacattcag
    34381 ttcgttggca ataatgttcg aaagggtggt ttttcccagc cccggaggtc cgtgcaacag
    34441 cacatgatcc agcgattcgg aacgcatacg ggcagcctga acgaatatcc ggagattttc
    34501 cacaattttg ttctgacctt tgaaatcgga aaaacttagc ggtcgcaaag cgttctcaaa
    34561 ttccttttcg gtatttttat tgtttcgtat atcaaatttt tcttccattg aaagccccct
    34621 agccccctga agggggaata tttgttgaat tacttttttt attgtttgtt cctaaagtcc
    34681 catccagggg attcaggagc gatggtcaat tctctgaaga cttcttccat tctttttgcc
    34741 aatggcttca atgtgagaat tttattgttg gtttttacag ctaagtcaaa aatgtcggaa
    34801 cggatgtctt tttcgcttgt aatgctgaac aatttactgt cttttgtatc aatattttta
    34861 attgatttta tcgaacctaa aacatctttt tctactttat tcaaaaattc cacttccaac
    34921 tgaaaatcat ttttaccaaa gttagaaatt tcctttacat ccgtataatc ggcgacaatt
    34981 tcacccttgt tgataattat cacccggttg caaacggctt caatttcttg catgatatgc
    35041 gtgctgaaga gtactgtccg gtttttcccg atttccctga taagattacg tatttcaacc
    35101 aattgattag gatccagtcc ggttgttggt tcgtcaagaa tcagcacttt gggattatga
    35161 attaatgcct gagccagacc cacacgctgc cggtatcctt tcgaaagttg tccgattttc
    35221 ttactgaatt caggtcgcag tcccaccaga tcaatcagct cgtcaacccg tttgtatttt
    35281 tctttaccaa tgccataggt ttcagccacg aaaagcagat attctttcac atacatatct
    35341 gtatacaacg gattttgctc gggcagataa ccaatattct tcttcgtttc aagtggatta
    35401 tcagcaactt ccatgccgca aacagctgca ttcccggttt ggtagtcgag cgctccagca
    35461 agaatcttca tggtggtggt tttacctgca ccgttaggtc cgagaaaacc gacaacttcc
    35521 ccggcatcaa tcctgaaact gatatccctg agtacctgct gattgccaaa tgacctggaa
    35581 atatgtgaaa tttgaataga catgcttagt tttccgcttt ctttacctga atgaaattta
    35641 caattaccga taagagaatg taccacatga taagcggaga aatggctttt ttccacagaa
    35701 tggcgattag caggacgctt ccacccagaa aaatgaattg tactttattg tctttccagc
    35761 tgagattttt gaatttcagc gaaaacatgg ggatttcagc gacgagcaaa taggagaaaa
    35821 acacagtcag tcccagtaag atatatggat ttgcaataaa ccattccgac cacgctaaag
    35881 ctattcctgc ccagaaaatg gcatttgcag gaaccggcaa accgataaac gaagaggttt
    35941 gtcgttcgtc aatattgaat tttgcgagcc ttaaagctga aaagatggtt aacagaaacc
    36001 ctacaaatgc atagttgtgt gataaatcgg gtgaaagcat atgaaaaatc agggctcccg
    36061 gcaccagtcc gaagctgatt acgtctgcca atgaatcaag ttccttaccc ataggagagt
    36121 aagctctgag tagccttgca gccataccat ccagaaaatc gaaaactgct gaaaaaatta
    36181 ccgcaagagc tacaccctca tagttcccgt taaaggcaaa ccatactcca acacaaccag
    36241 aaaacaggtt gagacaagtt accgtatttg gaatgtgttt aatcataatt aatcctccct
    36301 tagtgttggt aaaaaaaata ataaaaatgt atatacgatc tgaaaatcaa tattgtaatt
    36361 gaaaaagtat taatcaattc ctgtttagta gagctaatct gacaatttgc tgtccatcca
    36421 gatagttaca ggcccgtcgt tgatcaattc cacctgcatg tctgctccga attcgccggt
    36481 tttcaccggt tttttcaata gttgttccac ttcattgcaa aaatgctcat acaaaggaat
    36541 cgctttttca ggacgggctg ctttgatata agacggtctg ttgcctttct gagtttgtgc
    36601 gtgaagggta aattgactta taatcattat ttcgccgtca acctctgtta caggcaagtt
    36661 catcacacct ttctcatcgt taaagattcg cattttcacc agtttggaag ccagccagga
    36721 gctgtctttc tcatcatcgg catcttcaat gccaacaaat accagtaaac ctgtttttat
    36781 ttccgatttc agatgatttt caatcgttac agatgctctt tttacccgtt gtatcaatat
    36841 cctcattcga aagtgctgtt acatttaact tattaatcaa aatggatagc caatagccag
    36901 attgagtgca aaatcttctg ccttgatatt tgtgcgccat tgttcagtac gctccagtgc
    36961 aggattaaac aacctcaccc ccagatcgac acgcaacaca aagaaagaaa aatcggctct
    37021 gatacctgtt ccataagcaa ttccaagttg tccgagaaat ttatcaaaac gaaaaacacc
    37081 accgggttga gcctcgtaat tgcgtattgt ccagatattt cctgcatcca ggaagaacgc
    37141 accgtcgagt ttccagaaaa gtttggtacg gtactccata ttcagatcaa gttttatgtc
    37201 acccatctgt gtgttgtaat ccacgtaatt acccggactt ctgtacgttc ccggtccaag
    37261 ctgataggcc gtccagcctc tcacactgtt agcgcctccg gaaaagaagc gtttttcaaa
    37321 tggcattcgt tcagcatttc cgtacggtac tgctattccc agtccggcgt gataaaccag
    37381 ccggttgttt gcatcaaaaa tctgataatg ggctatctga aaatcagttt tgacatattg
    37441 agcatatcgg ataccaaaaa ctttgaaggc accggtcgaa tcacgctgcg cgttgagaac
    37501 ggagttcaat ccctgaagaa tatttcctgc tgtttcgata ccgtaattca tggtggtgta
    37561 gtttcttaac ggctgagcag cattgaaacc actgtacgag ccattgtaac cggttcgcat
    37621 gataaggtgt ggctgaaagt tgtaggcaaa tgcagggttt gaattgatgt aattctgata
    37681 ttcgccggta attttcggat aattcacata actgatatcc agcacgtcaa aattgtgttt
    37741 cacctgtagt ttttcactcc agctgtactt aaaacctgtt ccggtaacaa tacctgtata
    37801 ttctttcgga cggtcgcggt aactgaaagt agctgtaatt tcggtattag cacgtatatt
    37861 tcgcctcata tccagactcg aaaacggcag caggaaagtt ggaaatttca agccaaggtc
    37921 tgtgccgact tcaatggctt tttgtccggt gccctgatat tcgtaggcaa gtctgccttt
    37981 ggccgaaata gtttcagagc ctttgaaggc gttgcggtgt ccgtaattta tattgcctcc
    38041 aaatccccag taacccgacg aataggtgat ttcaccatcg gcggaaatgg tttgcagttt
    38101 attgggagta attacgatgt agcaatccag tttgtcgttt gcaatttcgc ggaaacttac
    38161 gtttacatat ttgatcgagc tcagcgaatt caacgagcta tatgtgcgtt caaccgaacg
    38221 gtcgccatag ggctttccgg ggacgatgag cgtatgttca atcagcacag tcggacgaat
    38281 gttgcgtttg ttttcataca ccagttcgta gtcaccaagg acaagtgtat ccagctccgg
    38341 cgttaaatta acagcatcag ttgatttatc cgattcgcag taaaaatgga ccttgttgat
    38401 ggtatattgc tgaaaaatac gctctttgat gctttcatcc gattttttca gctgttcatt
    38461 catttccagc accaaatcta ttctgtgtga aaccaggctg ctgtcggcat aataatgaag
    38521 taaatctttg gtaaagttgt aataaccaag ctctctgaat ttggaagtaa tccggtccct
    38581 ttcttcgtcg aaaacatcgc tgtcaaaaag tttacctgtt ttaatgaggc ttgcagcagt
    38641 gtctgatgca atatccgaaa gctcaggctg atttatatta accgaatagc tgttcagtct
    38701 gtacggttca tttgattgaa ttgaatattt tacatcagcc agtttttctt tcgggaaact
    38761 tacctcactg ttcactttca catccatata acccttgtta ctgaatagct ttgtaatttc
    38821 cttttcggta attgtagtca gtgatggatt gtagattacg ggttcctcgc cgattttttt
    38881 taatctccgg ttaatccatt tgctgctgtc agcgcccgcg aggttataaa tgcccagttg
    38941 cattttataa atcccaaata tctcgttgtt aggtgtttgc cggatgtatg tactgatatc
    39001 ttctttcggg atggctttgt tgtcagtttc aatttttaca tcattcagca aatgttcatt
    39061 gtcagggaca tatttcgttg gattacatgc cgaggcaagc aatccaagta caagtataag
    39121 aaacaatgaa ccttttgaaa atttcatatt atatactgag caatatcagt catactcaaa
    39181 aacttttgca aatataagct ttattctcgt tttctttatt agttttgtaa cgtaaaataa
    39241 gactgtaaga agaaattgaa cgaatatatt gcctgaatta tgatatcaaa atcgaagata
    39301 aaatggatcc gttcactgga aatgaagaag aacagggatg agcaaaatct gtttgttgcc
    39361 gaaggagtaa aacttgtttc ggatttactg ccacatcttc gctgccggta tctggcagta
    39421 tgtaccgata gttttgatat tgaacgtttt tcccgttttg ctgccgaaaa tgagcaaatt
    39481 actaccgatg aatataaaaa gatcactttt cagaagtcac cgcagggagt tttggctgtt
    39541 ttcgaaaaac ccgctcacct gtgggatgaa aaggaaacat gcagcaagtt gagcctggcg
    39601 cttgatgata ttcaggatcc tggaaatctg ggaacaatca tccgtttagc cgactggttc
    39661 agcatcgaac atgtattttg ttccacacat tcagccgatg tattcaatcc caaaaccatt
    39721 caggctacca tgggcgctat cgcgaatgtg aaagtgcact atgttgagct gccgttattt
    39781 ttgcgtcaac tggaaggaag aattcctgtt tatggtactt tcatggacgg ggaaaatatt
    39841 tacgatagaa atttgcaaag gaatgggata attgtaatgg gcaatgaagg acaggggatt
    39901 tctgctgaag tggagcaact ggtgactgaa cgattacaga ttccctgttt tgcaccggga
    39961 acagtcgcct ccgaatcgtt gaacgtgggg attgcaaccg ccattacggt tgccgaattc
    40021 agaaggcaaa catataagaa atgatataga atgagaaaaa caacctactt catcatttta
    40081 actgtgatag ctctggttct gtttgcctgg gcgactaatc cgaacgaaca gcagcatcag
    40141 gaagccgcaa ggattaagtt cggtgaaata gtcgaaaata cgatggctga tttaggcctg
    40201 aagaaaaatc tgctcactga ccttggattt gaaataggga acaggtatat cgacagttta
    40261 cttgatgact gtatttcggc caacaactat cttgtctgtt ccacaaccgc tttcaccatt
    40321 aacggctaaa aacatattat cggttttggc gctttcggca aagtccggat atccgataaa
    40381 gtggaagaaa gtctcaaaaa agaaataaga aacggaataa tcggaaataa tccgcttttc
    40441 atccgctaat cttcgatgta cagataatcg taatgtatca gcggtttctg atttttttta
    40501 ccgattagtt gtctggcttt ctcattattg tactgtactt ttcccactcc gattattttc
    40561 ccgtttgaac ccacaatttt tacaatatca tctttttcaa acgttcctgt aatttgggta
    40621 acgcctaccg gcagcaggct ggtggctttt ggacccgaaa gagctttttc cgcattctca
    40681 ttaatatgaa tctcaccctt ggcaaatcca tcgctgtgtg caatccattt tttaacgctc
    40741 gaaacatccg ttgctgatgc ttcaaagatc gtacagactg tatctttttg cttatcgagc
    40801 aaatcaatca gaatattctc ccgtttgccg ttggcaataa taacttctat tccttcgtct
    40861 gcaattttac gcgcaatgga agttttcgtc tgcattcccc cgcgacccag tgtcgatttg
    40921 gaagtctgaa taaagtcaga tatatcctcg cctttcctta ttttccggat aacggctgat
    40981 tccggattgg aaggagagcc gttataaata ccgtcgatat tgctcagaat aatcaaagct
    41041 tccattccga gcatggaggc tatcaggcct gagagttcgt cgttgtcggt aaacatgagt
    41101 tcggataccg aaacagcatc attttcattc acaataggta ttaccttgtt ttccagcatt
    41161 atgcggatgc agtttcgctg gttcagatat tgcgaccgtc cttcaaaatt agtttttgtg
    41221 gttagtacct gaccgacaac aattccgtgt tcgcgaaaaa gttcccaata atggttgatt
    41281 aattttgcct gtccgaccgt cgaaaataat tggcgctgtt cgactatatc catttttttc
    41341 gaaatgccca tcacgcttct gcccgaggct actgcaccgg aagatataag aaccagttgt
    41401 acgcccagtt tatgcagaac agatatctgg tcaaccagtg ccgacatacg tgtaatgtca
    41461 agggtgccgt ccggacgggt gagtacatta ctgccgattt ttacggctac ttttttatac
    41521 cggatattag tcttgtccat cagtgtttta tggtttttcg ttaaatatta cgtgcagcaa
    41581 ggttgttccc actactttca gtgttactgg atcgatattt tccatcgtgt cgttagtggt
    41641 atgccagtat ttaccaaatc cggtcatgct gtacggatca tactgaatga tgtctattgc
    41701 cggaattccc gccaactggt taacgtagag atgatcgtcg gtaattgcac cgcccggttt
    41761 gtttacaaaa taattgctga aacccagttc tgcagccttg gaccatacct tgtccactac
    41821 gtattttgca taattatcgg acagctgttc gcggtaaaat tttgcctgtg gagcgccgac
    41881 catatcgagc aatatgccgt aacgtgctct gtagttggga atatgtttgt ttttagccca
    41941 gtattgtgta cccaggcacc acgagtcttc atttccggca gatgctccgt aatcttcagc
    42001 atcgaaaaag atgatgtcaa cgcccactga aggtttttgt ctgttgagct ggcgtgctat
    42061 ttccagaagt actcccacac cgcttgcacc gtcgttagcg ccgagtaccg gagttttatg
    42121 atttttctca tccgtatcgt tatcggccca ggggcgtgtg tcccagtgtg caaaaagcaa
    42181 caggcgcgtt tcagcatccg gattgtacga tccgataatg ttttttgatt tcagcgtggt
    42241 gccatcgaag gcggtgaggc tggcttcctg cacggtgacc gttgcaccaa actgtttaag
    42301 agctgataca agataatctc cgcaggcttt gtgttgagca gtattgggta cgcgtggacc
    42361 gaaatcaacc tgtgttttca caaacttgta cgccgtatcg gctgaaaatt caggtaccga
    42421 tacctgaacg gtttctgctt tattttctgt cttatttccg gtgctcgaac atgaaaaagc
    42481 catgattatc agactaatga ataataggtt tcgcatagag atagttgatt gttaacagga
    42541 ttatatttaa aaacaaagat acaatatttt ttcagcacca gataagttat ggattttttc
    42601 aagatatgtt cattcgtgtt tttaatatcg tggtattaaa actatcttac cctgttgctt
    42661 aaaataatag taaaacgggt atgattgctg gtgtagaaat cacatattca tgtgcaataa
    42721 gaatatgaca aatgaacatt caaaacttaa ttctgataag aaatttaata caaaattgtt
    42781 aaaatatact taaaaacagg ttttataaca tatttgttta tttgttttgt actggaattt
    42841 gtttttactt tgcagacgtt aactaaacaa cacaatcttt tttatacctt agaaaaatta
    42901 actaatacct aaaaggaagg gctttgtgaa aagcccttcc tttttttaaa gtcattatat
    42961 ttttttcata gaacaaaaga aaaacgtttc gtgtctattt tttttaatac caaaatataa
    43021 atattccttc cctcttacta atataatgcc gtaccagcgc tttcttaaat aaatataaga
    43081 gttatcatct gttcatcgaa aagtgttatt tttgcacata ataaactgta aagtgtattt
    43141 attgatataa aaattacaaa ttaaaatata atattatgag ttttaacatc gttgttcttg
    43201 ccaagcaagt gcccgatacg cgcaatgtag gtaaagatgc catgaaagcg gatggcacgg
    43261 tcaaccgtgc agcactgccc gcgatcttta atccggaaga tctcaatgcg ctggaacagg
    43321 cacttcgtgt aaaagacaaa ataaaagacg cgacagtaag tgtgcttaca atgggacccg
    43381 gccgcgctgc cgaaattatt cgtgagagta tttatcgtgg tgccgataat ggttatctgc
    43441 taagcggtcg cgagtttgcc ggatctgata cacttgcaac ttcctatgct ctttcatgtg
    43501 ccattcgtaa gatgggtaaa gttgatctga ttgtgtgcgg ccgtcaggct attgacggtg
    43561 ataccgccca ggtaggtcca cagattgccg aaaaactggg tttaccccaa atcacctata
    43621 cagaagaaat ccttgacgtt gatgctgctt cacttacagc aaaacgcaga gtagaacggg
    43681 gagttgaagt ggttaaagga ccgcttccga tgcttgtaac tgttaacggt acggcagcgc
    43741 cctgtcgtcc gcgtaatgcc aagctggtaa tgaaatacaa gtatgcaaaa acctccaccg
    43801 aacttcagga aagcagttcg gattacaccg atttgttcaa ttcgcatccc tacctgaaca
    43861 ttaccgaatg gggtgcagcg gatattgaac acgatccgca atggctcggg ttgagcggtt
    43921 cacctaccaa ggtaaaacag attgaaagtg tagtgtttac tgccaaagaa agtaaacgta
    43981 tgggagtttc cgatacggaa attgaagatc tgataaagga actgattgca aatcacacta
    44041 ttgggtaatt tataaattga cacagacatg aataatatat ttgtatatct cgaaatagaa
    44101 gatggcgtcg ttggcgatgt gagtttggaa cttctgacca agggccgcag tctggccgat
    44161 cagtataaat gccacctcga agctgttgct g
//
LOCUS       DAOPYB010000007        42088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_793_length_42088_cov_11.076630, whole genome shotgun sequence.
ACCESSION   DAOPYB010000007 DAOPYB010000000
VERSION     DAOPYB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 42088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42088
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_793_length_42088_cov_11.076630"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PKH58_01100"
     CDS             complement(<1..913)
                     /locus_tag="PKH58_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX87652.1"
                     /translation="MKKKVLVIIALITSIQMYPQKILQLEECRQLALTHNKSLQMAQE
                     SVNAARELKKAAFTQFLPNFSANGTYAWNQKNLSLLSEDKYLPVYNLNADGSPNYAGS
                     WNNKWTQVAPSTYAPLDANGVPFDPKANPEKIQWKNSALLPKSAMEFDIHNIFAGTIG
                     FTQPIFMGGKIKELYNIATYGENLAKAQQENKITDLLLEVDEAYWRVVSVENKVALAK
                     EYRDLIAKVDSNVTIMIEEGVATKAESLKVKVKLNEAEMTLSKAEDGLSLSKMALNQL
                     CGIPLDETYKLADQDLETEKITQLIPIE"
     gene            complement(1037..1471)
                     /locus_tag="PKH58_01105"
     CDS             complement(1037..1471)
                     /locus_tag="PKH58_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX87653.1"
                     /translation="MVQLQNDELFDILVGKISATINRTFLRTFTAEGIDISIEQWTVL
                     ACLWNKDKVTQQTLCSLTAKDKPSMTRLIDKLERSNLVTRVSDHNDRRINLIHLTNKG
                     CALEQKATELVQKIVSKALNNITDEELNISRTVLKKIITNLL"
     gene            complement(1763..2275)
                     /locus_tag="PKH58_01110"
     CDS             complement(1763..2275)
                     /locus_tag="PKH58_01110"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNX87654.1"
                     /translation="MRIFLIGYMGSGKTTIGRLVAKRMNMNFTDMDELIERKAGKSVA
                     EIFSELGEDRFRELERNCLLEVAGYEQAIISTGGGAPCFFDNMEIMNSLGVTIYICLT
                     PKQLASRLRTTNLNDRPVLASYQGDDLEEFIASALQKRELFYNQAKIKVSGTDSQIAD
                     KIIKHLQGVL"
     gene            2512..3549
                     /locus_tag="PKH58_01115"
     CDS             2512..3549
                     /locus_tag="PKH58_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNX87655.1"
                     /translation="MSIKNSVLLIFTGGTISMSANPETGALRPADFESMQKLMPELGD
                     LGIDIQGIPLLPLVDSSDIGPDTWERIAMTIKDNYSLYDGFVVLHGTDTMSYSASALS
                     FMLEGLSKPVIFTGSQLPIGVLRTDAKENLITAVEIAAAHTNGHAMVPEVCLFFEDKL
                     FRANRTTKQNSEHFNAFRSFNYPELAEAGVHIRYFPNFIRKPDKNAVLKVRTKIDCNV
                     AILKIYPGITEQTVQGVLNIPQLKAVVLETFGAGNAPRFDWFYRALKSATKRGIIIVN
                     KSQCSTGSVEMGLYETSLNLLNAGVISGYDSTTEALISKLMYLLGENEDIETVKKLMQ
                     TSLCGEITVHP"
     gene            complement(3767..5965)
                     /locus_tag="PKH58_01120"
     CDS             complement(3767..5965)
                     /locus_tag="PKH58_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HNX87656.1"
                     /translation="MFFPVMAVDLPVIPKPNQVEQLSGNFDLTQAVQIRTSGIPRQSA
                     KHFKDYLSKAGFRSGKGHKALIELFTEKRKAHFVSDEAYRLRIEPGKISITAGNEKGL
                     FYGLQTLLQLTVEYGATKQIPALNITDSPRFAYRGMHLDVSRHFFSVDFIKKQLDMMA
                     YYKLNRFHWHLTDGPGWRLEIKKYPLLTDLAAWRTHKTWKDWWASPRHYVSKTDTGAF
                     GGYYTQDEAREIVRYAAERHITVIPEIEMPGHSEEVLAVYPHLSCSGVPYKNSEFCPG
                     NEETFSFLENVLTEVMAVFPSEYIHIGGDEANKEPWKTCPKCQKRMKDEKINDVDELQ
                     SYLIRRIEKFLTAHGRKLIGWDEILQGGLAPDATVMSWRGESGGIEAVKSGHDAIMTP
                     GAYCYFDSYQTDPSNQTEAIGGYLPLEKVYSYNPVSSSLTPAEAKHIAGVQANLWTEY
                     ISTPEQAEFMIYPRLLALAEVAWTEYENKSWNDFNRRVSEAIPFLQNRGYHPFTLSGE
                     VSFAQDIDSVGKKMLVTLTTEKYGKEIRYTLDGTLPISFSALYSGPLAVNDSAFITAQ
                     VYDRGKAVGKATGRRFDYHKAIGKKIIYNIPVNHYYPAGGSSSLIDGRTGGLTHSDGR
                     WQGFMTDGMDVTIDMQEVTPLKKIGARFMQNPGAWIYFPNEVVISVSDDNKNFTEIAR
                     MANPYDMKTAGTIFFNFQWTGNAKGQFIRFQGLPNNKEGTWVFLDEIVVW"
     gene            complement(6085..7113)
                     /gene="thiL"
                     /locus_tag="PKH58_01125"
     CDS             complement(6085..7113)
                     /gene="thiL"
                     /locus_tag="PKH58_01125"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNX87657.1"
                     /translation="MNRTEISEYGEFGLIKHLTEKIKLQNPSSVKGVGDDAAVLDYKD
                     KQVLVTTDLLLEGIHFDLTYTPLKHLGYKSAVVNFSDIYAMNGQPKQMTVSLGVSKRF
                     TVEDLEEIYAGINLACEKYGVDLVGGDTSASLTGLIISITCIGEGEKDKIVYRNGAKP
                     NDLICVSGDLGSAYMGLQLLEREKVVFSGQQENVQPDFEGKEYILQRQLKPEARKDIV
                     DALHKKGIVPTAMMDISDGLSSELIHICSQSNTGCRIYEDKIPINYEAAVMAEELNMN
                     LVTAALNGGEDYELLFTVSLDDYDKIVTMEGIGIIGHITKPELGLCLVGREGEEIALK
                     AQGWNSLK"
     gene            complement(7194..7646)
                     /gene="rpiB"
                     /locus_tag="PKH58_01130"
     CDS             complement(7194..7646)
                     /gene="rpiB"
                     /locus_tag="PKH58_01130"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNX87658.1"
                     /translation="MKPFNELRIAICNDHAGYQLKTFILKYITEKAPALLKDFGCDSP
                     ESCDYPDYAHPMATAIENGLFDYGISICGTGNGISMVLNKHQGIRAALCWNEEIAVLG
                     RLHNNANIISLPARFISEEQALKMIDAFFSTDFEGGRHQRRIDKIPVK"
     gene            7830..8753
                     /locus_tag="PKH58_01135"
     CDS             7830..8753
                     /locus_tag="PKH58_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87659.1"
                     /translation="MKNFSRISFILLIIVTAIGAPACNKSKTTAQNSPTNDTLPRIVS
                     DKMRFTESTYPYRDGFIVANFGSSELNPLNLEGKGYLLFLKETTVTMLIPPDGNLNAP
                     RGMYERNGWLFIADVGKIVVYNLNSLNVKPQTVWFPKEDVMVNDLAAHGDTLFATVSN
                     TGRIFTVNIKDPARVSREKPVKYINIPGPNGIAVAFGKLYICSFSQQGKPDAANVVYV
                     VNDLKKPVPEKIITQPGNYDGIAVSDGGTKLYVSNWSPAGVIEIDLSTKAVKKINLPV
                     EVTGPADFSLWDGKLIIPDLVKSRVIMMKMP"
     gene            complement(8959..10110)
                     /locus_tag="PKH58_01140"
     CDS             complement(8959..10110)
                     /locus_tag="PKH58_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX87660.1"
                     /translation="MAKILVIDDERSIRNTLKDILEFEKHKVILAENGKQGLDAALND
                     LFDIIFSDIKMPEMDGIELLEQLKEKDVEAPVVMISGHGNIETAVDCIKKGAFDFIEK
                     PIDLNRLLITVRNALEKNNLVTETKTLKKKVAKKLQMVGNTPCIERVRDMIHKVAPTD
                     ARVLITGANGTGKEIVARLLYEYSNRVQGPYVEVNCAAIPSELIESELFGHEKGSFTS
                     AVKQRIGKFEQADNGTIFLDEIGDMSLSAQTKVLRALQENEISRVGSDKNIKVNVRVI
                     AATNKDLKAEIEKGNFREDLFHRLNVIPIHVPTLDERKDDIPLLVTHFCELICAEQGI
                     QLKTFDTKAVKALQERSWTGNIRELRNVVERLIILGGNKITKEDVEMFS"
     gene            complement(10183..11451)
                     /gene="purD"
                     /locus_tag="PKH58_01145"
     CDS             complement(10183..11451)
                     /gene="purD"
                     /locus_tag="PKH58_01145"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNX87661.1"
                     /translation="MNILLLGSGGREHALAWKMAQSPKLTKLYIAPGNAGTKQAGENI
                     PVNISDFQAVKKTVLEYNINLVVVGPEEPLVNGIADFFEADGDLNTIPIIGPNRVAAQ
                     LEGSKDFSKQFMHRHNIPTARYLSVTAENINAGIDFLHELKAPYVLKADGLAAGKGVL
                     IINNTNEAIDELKSMLEGKFGKASQTVVIEEFLSGIECSVFVITDGKNYKILPEAKDY
                     KRIGEGDTGLNTGGMGAVSPVSFADETYMQKVEERIIRPTVEGLQADGITYKGFIFFG
                     LIKVDNEPMVIEYNARMGDPETEVVMLRLKSDLVDLLEGVAAGNLDEKTIEFDERSAV
                     TVMLVSGGYPDVYEKGKVITGLEDVKDSIAFHAGTTEKDGNILTNGGRVIAISSYGTN
                     KDEALAQSFRNAQLVDFDKKYFRRDIGFDI"
     gene            complement(11489..12667)
                     /locus_tag="PKH58_01150"
     CDS             complement(11489..12667)
                     /locus_tag="PKH58_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PNGase F N-terminal domain-containing protein"
                     /protein_id="HNX87662.1"
                     /translation="MAKTAPICGDLETNLFTDLNIQFNPAKYPDGVNEAEGFIRLANG
                     RIAIKKIVLPEYKRNVQITAKLRLTSNGDPWDKSGSCFIIPKESLINMITVARGTAKY
                     PDIDSTKLEKLVGTIVGKDYLPTVELMRFMTPFGVGHFSAEKDSTSYKRRPVYIDGWA
                     QEAAWQQDITDLYPLLKGEVYVGIYIDTWTEKGYVVNFEINVEESKIKQDVMPIRKVM
                     PLINTVYYLGQTYPDIFARKDITVPFSLPRKAKNVRLKYIVTGHGGHSGGDEFVQKEN
                     ILSVDGAVVHRFIPWRNDCASFRRFNPTSGVWLRKRRIAYISDEGRAEKEIEEPIASS
                     DLSRSNWCPGSDVPPVVVPLIDIRSGEHTLTISIPNAQAIEDNKLNHWLVSAYLVWEE
                     "
     gene            complement(12715..13518)
                     /gene="cdaA"
                     /locus_tag="PKH58_01155"
     CDS             complement(12715..13518)
                     /gene="cdaA"
                     /locus_tag="PKH58_01155"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNX87663.1"
                     /translation="MAFNIGIKDIIDIFLTAFLLYGAFRLLKRSGALNVFLGIMTFVI
                     IWFVVSKVLRMELLGEILDGLVNVGAFALIVLFQEEIRRFFSRLGSRKNWGILNYFKK
                     FLKGTENEQIRTDYDLIQIVIACRNLSKTATGGLIIITRSNDLEFYTQSGEIIDSRIT
                     SRLIENIFFKNSPLHDGALIISNRRLKAAACILPVSRNQSIPKRLGLRHRSALGISEI
                     SDAIAIVISEETGHISWAINGKLTINGKPEELEHILSEEIAEYSKTTNP"
     assembly_gap    13539..13638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13761..14606)
                     /gene="folP"
                     /locus_tag="PKH58_01160"
     CDS             complement(13761..14606)
                     /gene="folP"
                     /locus_tag="PKH58_01160"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNX87664.1"
                     /translation="MNKIPFQLTLNGRLFDLSKPAVMAIANLTPDSFFSGSRLFSERE
                     VLNRVEKAFAEGASILDVGAYSTRPSAAHITEEEELERLRLPLKAIRKHFPDIVISLD
                     TFRSGVARWGVEECHVDIINDISGGTMDDMMFETVAVLNTAYILTHTRGTPQTMQQLT
                     KYENMMSEMLFYFGKKVAQLVHLGVKDIIIDPGFGFAKTLDQNYELLKKMSYYKELNL
                     PVLFGISRKSMIYNLLGNTPEEALNGTTALNMLGLMNGADILRVHDVKEAVEAVKLFE
                     KYNNS"
     gene            14988..16544
                     /locus_tag="PKH58_01165"
     CDS             14988..16544
                     /locus_tag="PKH58_01165"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX87665.1"
                     /translation="MKLINVAKLLTFTFATLVIVSCGSKSGASAGFGSGKKSAISNTT
                     GWRYNDEKMTGFGVKKNYKAQVPTGMIAIEGGTFTIGEKGEVITGERNNLRRRITVMS
                     FYMDQYEISNVNWREYTHWMKTVFQQTAPQLVERAQPNGEVWREPLAYNEPLLEHYFT
                     HPAFDEYPIVGVTWEQAMDYCQWRTDRVNELALINAGVIEAPDFSSLKKITNADSIAN
                     NFVFNTQKYIFHSKYNPKPGKKSLKDIYGQTRKADMSDGIMFSNYRLPTEAEWEFAAY
                     AIKSDSKDGLVKEGKVYPWNGSQLRYNKKGKYLGEMQANFVRGRGDMMGTSGSLNDKA
                     AITNAVNSYLPNDFGLYNMAGNVNEWVMDVYRSTTFDEMAEYNSFRGNEYRTYLATAK
                     DALGNYTLKIDSLGRVATGVLPGDDKRSFHDGDETTKINTDFPLITEPEGLEALANNE
                     KLDPTDILAPKISDRTRVYKGGSFKDRAYWLNPSTRRALDQDKSANDIGFRCAMSMIG
                     DIRSQSTKKK"
     gene            16696..18093
                     /gene="asnS"
                     /locus_tag="PKH58_01170"
     CDS             16696..18093
                     /gene="asnS"
                     /locus_tag="PKH58_01170"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNX87666.1"
                     /translation="MQRTKIVDVLKRTDYGVDVNIKGWVRTRRGNKNVSFIALNDGST
                     IHNIQIVVDLAKLDEEKLKTVTTGACISVTGKLVESQGKGQTVEIQADDILIYGGADP
                     ETYPLQKKGHSLEFLREIAHLRPRTNTFGAVFRVRHHMAIAIHKFFHDRGFFYFHTPL
                     ITASDAEGAGQMFQVTTLDLENIKLTEEGKVDYSDDFFGKPANLTVSGQLEGELAAMA
                     LGAIYTFGPTFRAENSNTPRHLSEFWMIEPEVAFNEIEENMQLAQDFIQYCIQWALDN
                     CMDDLKFLSEMYDKELIERLRFVVDNDFVRLPYTEGIKILEEAVSKGYKFEYPVGWGT
                     DLQSEHERYLVEQHFKKPVILTDYPQEIKAFYMKQNDDGKTVRAMDVLFPKIGEIIGG
                     SQREENFEKLRTRAASMGVPEKDIWWYLDTRRFGTAPHSGFGLGFERLLLFVTGMTNI
                     RDVIPFPRTPKNADF"
     gene            18132..19982
                     /locus_tag="PKH58_01175"
     CDS             18132..19982
                     /locus_tag="PKH58_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNX87667.1"
                     /translation="MMEKIKVKELDQVVVRFAGDSGDGMQLTGSIFSNISAIIGNEIS
                     TFPDYPAEIRAPQGTVGGVSGFQVHIGSGKVYTPGDDADVLVAMNPAALKVNAKHVKK
                     GGVIIFDQDSFEKNDLVKAGFVSDDPFTEMSVSETISLVPAQLTTLTQKSLEGFPLDN
                     KAILRSKNMFALGLICWLFNRPMEQAVHFLGNKFGKKPSVLEANVKVLTDGFNYGNNL
                     HLSVPTYTIEKSDDVPHGKYTSISGNTATAYGFIAAAEKAGLELFLGSYPITPATDIM
                     HELALRRDLGAKVVQCEDEIAGVTTTIGAAFAGCLAVTNTSGPGLALKSEGINLAVMA
                     ELPIVIVDVMRSGPSTGMPTKTEQTDLLQALYGRNGESPLVVMAPGTPDDCFQYAYMA
                     SKIALEYMTPVILLTDAFLGNGTSLWNLPKLADMPEIKPNYVQPGMEGCKVTFRNEET
                     KVRYWAIPGTPGFEHRNGGLEKDYEKGSISTDGENHQKMVETRAQKVENVANSIPDIT
                     TYGSDDAELLVIGWGGTRGHLMSAVSDMNEKGEKIALAHFNYINPLPKNTAELISKYK
                     KVVVCELNNGQFATFLRSKVPGVNFLQYNKVQGQPFTVAELKEQFELILK"
     gene            19993..21006
                     /locus_tag="PKH58_01180"
     CDS             19993..21006
                     /locus_tag="PKH58_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNX87668.1"
                     /translation="MEEIKQYTPKDFKSDQYVRWCPGCGDYAILNSLQKVMADMGVAP
                     QDIAVISGIGCSSRLPYYINSYGFHTIHGRAAAIATGVKIANPKLTVWQISGDGDGLA
                     IGGNHFIHSVRRNIDINVLLFNNQIYGLTKGQFSPTTKKGMVTKSSPYGTIERPFRPA
                     ELTFGARGTFFARLLDVDLKNAQETISAAARHKGTSIVECLVNCVIFNNGAHGWIAEK
                     ETRPDRIIFLEHGKPMIYGVNRDKGLMLDGFELKAVTIGEYGITEKDLLVHDAHCEDI
                     TLQYKLAMMEGPEMPIAMGVIRDVQTETYDQAMENQIAEVKEKSKIKCFDDLVGSLEQ
                     WEI"
     gene            complement(21057..21788)
                     /locus_tag="PKH58_01185"
     CDS             complement(21057..21788)
                     /locus_tag="PKH58_01185"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNX87669.1"
                     /translation="MKSTAFYIASTTSTNLLLKQKMRQEDLPEGYIVYTDFQTAGKGQ
                     VGNSWESEPNKNLLFSLLLYPRSIKADKQFILSQITGLGVKKVLDKFTRDISIKWPND
                     IYWKEKKIGGILIENSLIRDCINTCVIGIGLNVNQEEFFSDAPNPVSLKQITNIDTNR
                     EELMMLIRAEIMDLYTHSAPEFIHREYLHSLFRKNGYHRYLETETKTIFLAETENILP
                     DGKLILRTESDDLKEYYFKEIQFIL"
     gene            complement(21785..23191)
                     /gene="dacB"
                     /locus_tag="PKH58_01190"
     CDS             complement(21785..23191)
                     /gene="dacB"
                     /locus_tag="PKH58_01190"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNX87670.1"
                     /translation="MKSKILILFLAIAIIPVTASNPVKHFINNDLLRNANISITVKKL
                     NTNKTVESFRSNNPVVPASTQKLITTATALELLGPEFRFETTLETDSPICPDGTLNGN
                     LYIHGSGDPTLGSAMLGDTAFLVEWVKKIQLAGIKSIKGAIVADATRFDTEGVNPKWL
                     WEDMGNYYGAGAYGISYKDNLYTLEFRSGEIGTTPQIVKMMPEIPGFEFKNNLKSSAI
                     RSDSAYFYGAPFSDHRTIYGEIPANRSSFKVKGDIPRPGLLLARDFTKALSVAGIEVS
                     RTATETEVRNKQRNVIYTHYSPQLRDIIREINVQSNNHYAEHVFRYLSLINDTVATSK
                     GSVTVIKKFWASRSLPVDQLYIYDGSGLSPVNAVSSGFMAGLLEYMDKSAYSQDFRAS
                     LPVAGTNGTLKSFLKSTALEGKIQAKSGTIHRVKCYAGYITLDNKNYVFSIMVNNPNG
                     TLSAVTKKIEEFLLSVCK"
     gene            23339..24124
                     /locus_tag="PKH58_01195"
     CDS             23339..24124
                     /locus_tag="PKH58_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX87671.1"
                     /translation="MKEINELALDDYSVYYEKTEYPENPEEPVLIFLHDSWGCVGMWG
                     DFPEKLMKIAGLNAFVYDRRGYGRSSPFGTAQRDKNYLHQEAGELIRVMDKANIRKAV
                     LYGHSDGATIALIAAAKYPERIVAVIAESTHTFIEEKGKTAVLESRERAKHNSLLQTL
                     EKYHGDKTGELFRRWHETWLSDNFADWSIIPLLCDIQCPVLAFRGKDDPYDSIEQQNV
                     LKSEMSSPLTTAVIPDAGHSPRIDNETVTMKLIKSFLSCNSFE"
     gene            24162..24542
                     /locus_tag="PKH58_01200"
     CDS             24162..24542
                     /locus_tag="PKH58_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNX87672.1"
                     /translation="MGLFSALMGNAGTIDPAKIEKEFGKLLTEDEVIEMGFKLIRDTF
                     IFTSKRLIMINVQGITGSKQEYLSVPYKSISKFSIESAGPFDLDAELKIWISSEQNPS
                     VTRNFNKSVNVYDVQKILAHYVLK"
     gene            24552..24908
                     /gene="folB"
                     /locus_tag="PKH58_01205"
     CDS             24552..24908
                     /gene="folB"
                     /locus_tag="PKH58_01205"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNX87673.1"
                     /translation="MAFITLQNMKFHAFHGCLEHEKKLGNTFLVTLRMELDTSIAAET
                     DNLDDTLNYQLVYDVVKQEMEKPSDLIEHAGQRILDAVFNNFPKIQSLHIQLTKLNPP
                     LGGSVESVIIELDKKR"
     gene            25039..26577
                     /locus_tag="PKH58_01210"
     CDS             25039..26577
                     /locus_tag="PKH58_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNX87674.1"
                     /translation="MSENIKQSHPKGLYFVFATGMSERFSYYGMRAIFVLYLIKALLM
                     DKEFASAIYGNYTGLVYLTPLIGGYVADRYLGMRRSIFWGAVLMVAGQLFMFASALNY
                     QSTELARWLMYCGLAGLIFGNGFFKPNISTIVGQLYERGDKRLDSAYTIFYMGVNVGA
                     FLSPLLCGFLGDTGNPADFKWGFLVAAVMMGLSLILYITQKNKYLIGPDGTAIGIHAP
                     RKAEKAKEKNNELSKEKLNVKKMMVLAGGAVLLFSLFMVLFDKDVIGAVIYTACIVVP
                     AYVITDSSLSKIEKQRIWVIFIIAFFVIFFWSAFEQAGASLTYFADEQTDRNIFGWEI
                     PASWFQSFNPIFVVAFAPLFSMMWLRLGRKNMEPASPTKQAIGLFLLSLGYLLIAFGV
                     KGAAPGAKVSMLWLTGLYFIHSMGELALSPIGLSMVNKLSPVRFASLMMGVWYLSMAT
                     ANKFAGVLSGLYPEAGKPKSFIVFQITDLYDFFMVFVIMSAIASVILFALSKTLQKLM
                     HGVR"
     gene            complement(26611..27105)
                     /locus_tag="PKH58_01215"
     CDS             complement(26611..27105)
                     /locus_tag="PKH58_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNX87675.1"
                     /translation="MKKIGLLTDTHGLLDERIYHYFKETDEIWHAGDWGSMEVVSKLQ
                     AFKPVRGVWGNIDGQDIRSIFPKHNRFLCEEMDVWLTHIGGYPGKYDPSVRTEIQANP
                     PKLFICGHSHILKVIYDPKLELLHINPGSAGKYGFQKVQTIVRFVIDGNKIKDLEVIE
                     LKPL"
     gene            27285..28211
                     /locus_tag="PKH58_01220"
     CDS             27285..28211
                     /locus_tag="PKH58_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNX87676.1"
                     /translation="MRIFLKIPTFAILFTDFRSVKKQLKHIISDFMQRMRFKYRVSIL
                     NENTLEETWHTRLSRFSVLLFVSAFFLTTFILLTFLIFITPLRHYLPGYGESGNRITI
                     VQQSMTTDSLMHQLELQQAYLSVLKDIINEKPQNDTIKSLDSIMLRERATVLLEKSKR
                     ENEFVKDFEESQKFNISSISARSGEKMYVFFRPVRGVIASKFEPQDGNFGVSVITSSQ
                     ENVMSVLEGTVIYAAFTFDFGWVIEIQHENGYISIYKNNTRLLKSTGDMVKAGETIAV
                     TGDEHNVNERKHFYFEVWKQGIPLNPQDVIVF"
     gene            28238..29401
                     /locus_tag="PKH58_01225"
     CDS             28238..29401
                     /locus_tag="PKH58_01225"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNX87677.1"
                     /translation="MTEKKKNIAILGSTGSIGTQALEVIRDNPDKFEVFALTANNNID
                     LLIEQACEFQPNVVAIANEKHYLQLKQALADQQIKVFAGIDAISQIVEMEPVDLVLTA
                     MVGYAGLKPTIQAIKARKTIALTNKETLVVAGEIIQELAIQNKTAILPVDSEHSAIFQ
                     CLVGEGDNAIEKVILTASGGPFRNKTIDELKNVTSRQALKHPNWDMGAKVTIDSASMM
                     NKGFEVIEARWLFGLNPAQIDVVVHPQSIIHSMVQFTDGSVKAQLGMPDMRLPIQYAF
                     AYPDRIKSNFPRVDFTKYNELTFEKPDIKRFRNLALAYQAMENGGNMPCILNAANEIV
                     VAAFLKDEIGFLEMSDIIENAMQKATFIKKPTYDDYVMTDAQVRELTLKEIKN"
     gene            29448..30932
                     /gene="rseP"
                     /locus_tag="PKH58_01230"
     CDS             29448..30932
                     /gene="rseP"
                     /locus_tag="PKH58_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNX87678.1"
                     /translation="METFLIRALQLILSLSILVVLHEFGHFVFSRLFKVRVEKFYMFF
                     NPKFSLIRAKKINGKWNVKFFASNIPANERMKLDSNGQPVMDGKNSVMEPVPLSEMSE
                     NDWRRYPETTEWGIGWVPLGGYCKISGMIDESMDKTQMAQPPKPWEYRSRTVWQRLPI
                     IIGGVVVNFILALVIYAAVLFTWGKEYIPLQNAKYGLSYSKSMTDIGFQNGDKILKVD
                     GQEVRTRADAIEKILIDFAKTVTVERGNQILDIKIPEGLTKKILAAGEANLIDIRYPF
                     VVAEVMKGSPAEKAKLIPGDSVIGVNGKEMNIFQDISAELSASKNSLITVTYVRNGIT
                     ENTEIQINEDGKLGVYMNPPTKYIQSEKEEYSFLKSIPAGIKMGVETLSSYIKQFKLV
                     FTKEGAKSLGGFGSIGQLFPPVWDWQAFWMMTAFLSVILAFMNFLPIPGLDGGYVLFL
                     LYEMITGRKPSDKFLEYAQSVGMILLLILLIYANGNDIFKAFFK"
     gene            30944..31303
                     /locus_tag="PKH58_01235"
     CDS             30944..31303
                     /locus_tag="PKH58_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HNX87679.1"
                     /translation="MITVLCYPKCGTCRKAEKWLKENNIEYTYRPIKEQNPTSDELKQ
                     WLGKSGLTINKFFNTSGLLYKKMNMKEKVKQLPEDELLRILASDGLMVKRPILLNGEK
                     VLVGFREEEWKEMFSVR"
     gene            31369..31845
                     /gene="ybaK"
                     /locus_tag="PKH58_01240"
     CDS             31369..31845
                     /gene="ybaK"
                     /locus_tag="PKH58_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNX87680.1"
                     /translation="MSKIQKTNVARLLDKAGISYELIAYEVDETDLSAVHVAAELNEN
                     PKQIFKTLVLEGNKTKYFVCIIPADEEVDLKKTAAVSGNKNCDLILQKDLLPVTGYIR
                     GACSPIGMKKQFPAFIHNSCSEFPYIYVSAGQRGLQIRIAPQDLIQISQARPAQLI"
     gene            31959..32396
                     /locus_tag="PKH58_01245"
     CDS             31959..32396
                     /locus_tag="PKH58_01245"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNX87681.1"
                     /translation="MKKLIMSLMLLCLIAAASFAQTDKIVGRWKTIDDKDGSEKSVIN
                     IYKSTNGKYYGKIEKLFKEPEKLCTECEGTHKNKPVLGMVIVNNMTESEGTLTGGTIL
                     DPKDGKVYQCNISYNAKKANLKVRGSLDRKGIIGRTQYWVKVK"
     gene            32548..33783
                     /locus_tag="PKH58_01250"
     CDS             32548..33783
                     /locus_tag="PKH58_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase-maturation protein"
                     /protein_id="HNX87682.1"
                     /translation="MSKTISFNPLTLPTYVMAKPAGSLCNLNCSYCYYLEKEKIYPNR
                     KKTEMSNELLELFTKSYIEAQPVKEVLFTWHGGETLLRDISFYRKALAFQRKYGRGRI
                     INNTLQTNGVLLNDEWCTFFKDNNFLIGISIDGPEHCHDVYRKNKGDRGTFAQVMRGI
                     GLLQKHEVDFNTLSVINDYNVLFPLDVYRFLKEIGSQYMQFSPIVERVSDYRPDGLKL
                     LPPEENIEAELASWTVNPVEFGRFYIDIFDEWVRNDVGTYFVQLFDSTLANMVGEQPG
                     VCIFAETCGHAGALEFNGDLYACDHYVFPEFKLGNIRSQTIFEMMFQERQLQFGADKR
                     DKLPLQCRKCDVLKFCHGECPKNRIARTPSGESGLNYLCEGYKAFFRHALPYMEFMAN
                     ELYFERPPINVMHFARGRH"
     gene            complement(33828..34841)
                     /locus_tag="PKH58_01255"
     CDS             complement(33828..34841)
                     /locus_tag="PKH58_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX87683.1"
                     /translation="MTFNHLHYILRVLKTGFTPRKILNAIKLAVSYQLSFFGIQTRWK
                     YSPWFISIEPVNGCNLHCPECPVGIRTERIEAVNINEKMVHHLIDELEQTLSHVIFYF
                     QGEPFINRNFTNLVRYAHNKNLLTSTSTNAQLLTDQTARKVVESGLDRLIVSIDGATQ
                     ETYEAYRAGGQLKKALQAVENVIKWRNELKSRTPLIEIQFLVLKTNEHEMEAMKLLAK
                     KLKADKLTFKSAQLYDFVNGNSLLPDNKRYARYELRSDGKYHLKNSQPNRCKRLWSGS
                     VVTSRGDVLPCCYDKDRSFVFGNLNEKSFGECFSSHKAAEFRKEMLHNRKQFDMCRNC
                     NEK"
     gene            35024..36721
                     /locus_tag="PKH58_01260"
     CDS             35024..36721
                     /locus_tag="PKH58_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX87684.1"
                     /translation="MNKKILIAVSLVMAIVFVSCNKNMKALDASYFKTTPNPLEEKAG
                     KVDATVTGTFPVKYFNKKAVVTVTPVMKFNGEEVKGQSSVFQGEKILDNNQKIAYKAG
                     GTYTMKPSFFYIPEMAKSELYLNFDVVQKKKNYTLPAVKIADGVLATSELVAVKDIKP
                     ALIADKFQRITQENQNADILFLIQQAQLRNSELKAEDVKAFTAKVKEARDAQNKEVTS
                     LEVLGYASPDGAVDLNTNLAEKRQKVTKDYVNKELKKLKSNVTVDSKFTAEDWEGFQN
                     LMESSNIQDKDLILRVLSMYQDPEQREREIKNLSTTFKTIATDILPQLRRSRMKLTVD
                     VTGKSDEQIAQLAATSPADLTVEELLYAATLTKTETEKAAIYQKVISLYPTDARGYNN
                     LGLVNYEMGKIAEAQKLYEKALAIEPQSADANYNVGLISLSKGAITDALAYFGKAAGT
                     TGDLNSALGTTYIAQGDYTKAKNVMTEANTNNTALAQILCSDYNAARRTLAAVAAPNA
                     MTAYLGAIIGARTNDRDAVYNNLKTAVQKDISLAKKALTDLEFSKYFTDASFLSILK"
     gene            36988..38145
                     /locus_tag="PKH58_01265"
     CDS             36988..38145
                     /locus_tag="PKH58_01265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNX87685.1"
                     /translation="MSVFSDIIGQHTIKERFIRTVREQRIPHAQLLYGPEGVGKLALA
                     IAYAQYISCTNRTETDACGVCPSCVKYKQLQHPDLHFVFPIIKPAGKSSIICDDFIGE
                     YRQMLISDRYFSVNDWYEKIGEGAKQGMIYSGESVEIIRKLSLKTYESEYKVMIIWLP
                     ELMNIDCANKLLKILEEPPAFTVFLLVSNNPDANITTILSRTQHVKVPRLEEHEIVDA
                     LISRYPESNKADIENTARVAEGSFLQACRILNEKGESSVNFNRFVEIMRLAWLVGNKK
                     DHQSLKTLKKWSDNMASADVGRERQKKFLNYAQNMVRENYIYNLRQPTLNYMTDYESK
                     FSANFSPYINERNVEDLMKEFALSERQIEQNTNAKMVFFDLVLKIIMLLKR"
     gene            38343..39407
                     /gene="ricT"
                     /locus_tag="PKH58_01270"
                     /pseudo
     CDS             38343..39407
                     /gene="ricT"
                     /locus_tag="PKH58_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            39499..39972
                     /locus_tag="PKH58_01275"
     CDS             39499..39972
                     /locus_tag="PKH58_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNX87686.1"
                     /translation="MIKKINNFFLLIAVAVAVSSCNQKDVYFSYQTVSASGWNKDSLL
                     TFSVGISDKTMPYNMYINVRHHGNYPYQNLWLFLENKDVKGKVTKDTIECYLADEFGK
                     WLGSGAGALKEMPVLYRQQVFFPDSGTYQIKVGQGMRDSILVGINDIGIRVEKAN"
     gene            39976..40725
                     /gene="trmB"
                     /locus_tag="PKH58_01280"
     CDS             39976..40725
                     /gene="trmB"
                     /locus_tag="PKH58_01280"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNX87687.1"
                     /translation="MGKNKLAKFAEMDTFPHVFQVPSASLLDGNGFELKGKWNEAFFK
                     NSNPIVLELGCGKGEYTVELARRFPDKNYIGVDIKGARMWTGAKDSFVNEIKNVAFIR
                     TNIEMIFHFFATGEVSEIWLTFPDPQMKKTTKRLAATNFISSYLKFLKPDGVVHLKTD
                     SNFMFTYTCAMVELNNFPVIGHTDDIYAGEHEKNPVLGIKTYYEQQWLERGLTIKYIA
                     FHPLAGKTLLEPDIDIEQDIYRSFKRYKRQQ"
     gene            40783..41889
                     /locus_tag="PKH58_01285"
     CDS             40783..41889
                     /locus_tag="PKH58_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNX87688.1"
                     /translation="MTLYPNLILEALKHVRYPGTGNDIVSSEMVQDNIRIEDNKVSFS
                     LLFEKPNDPFAKSVVRAAEQAILTYIGEDVEIKGNISIVTKELPKPKPASILPDVKNI
                     IAVFSGKGGVGKSTVSSNLAIALASLGYKVGLLDADIHGPSIPKMFGVENERPFVEEI
                     DGKQLMKPIEKFGIKLLSIGFFVDPENALVWRGSMASNALKQLITDTMWGELDFFIMD
                     LPPGTGDIHLTLVQTMGITGAIAVTTPQDVALADARKGINMFTGEKVNVPVLGIIENM
                     AWFTPAELPENKYYIFGKDGGKKLAEELGVPLLGQIPLVQSIREGGDAGMPIAINDNS
                     LTSMAFHHLAEQVVERVEYRNKTLIATQRVEIVK"
BASE COUNT        12134 a         8585 c         9197 g        12072 t
ORIGIN      
        1 cctctatcgg aatgagttga gtgatttttt ctgtttctaa atcctgatca gctaacttat
       61 aagtttcatc aagcggtatt ccgcatagtt gattcagtgc cattttcgag aggctcaggc
      121 catcctctgc tttggatagt gtcatctctg cttcgttaag ttttaccttc actttcagtg
      181 attcggcttt ggtcgcaacc ccttcttcga tcatgatggt tacattcgaa tcaacctttg
      241 caatcagatc gcggtattcc tttgccaggg caaccttgtt ttcgactgaa accacccgcc
      301 agtaagcttc gtccacttca agcaacaaat ctgttatttt attttcctgc tgagcctttg
      361 cgagattttc gccataagtg gcaatattat aaagttcctt tattttgcca cccatgaaaa
      421 tcggttgtgt aaagccgata gtccccgcga agatattgtg aatgtcgaat tccatggcgc
      481 ttttgggtaa taacgcgctg tttttccact gaattttttc gggatttgct ttggggtcaa
      541 aaggaactcc gtttgcatcc agcggagcat aagtggaagg tgctacctgt gtccatttgt
      601 tattccatga acccgcataa ttgggactcc cgtcagcatt caggttgtaa accggtaaat
      661 acttgtcctc cgataaaagg gaaagattct tttgattcca cgcataagtc ccgtttgccg
      721 aaaaatttgg caaaaactgc gtaaaggcgg ctttttttaa ttctcttgcc gcgttcacac
      781 tttcctgagc catttgcaaa ctcttgttgt gtgtaagcgc caattgtcta cattcttcca
      841 gttgcagtat cttctgcgga tacatctgta tggaagtgat taaagcgata ataactaata
      901 cctttttttt catacttaaa taatagtttt tagttttcgt aggaaactcg tcccgaaaaa
      961 tcgggattcg attcattttt tatataattt gattaaataa ataatagttg tctatgcaac
     1021 aaaaaagtca aaaaaactag agtaaatttg taattatttt tttcagaaca gttctgctga
     1081 tattgagctc ttcatccgtg atgttattga gtgctttact aacaattttc tgaaccagtt
     1141 cagttgcttt ttgttccaaa gcgcaacctt tattagtaag atgaattaaa ttaattctcc
     1201 ggtcattgtg atcggaaaca cgtgttacca ggttgctcct ttcaagtttg tcgataagtc
     1261 ttgtcatgct tggtttatct tttgctgtca agctgcaaag tgtttgctga gttactttat
     1321 ctttattcca taagcaggcc agtactgtcc attgctcaat ggaaatgtca ataccttcag
     1381 ccgtaaatgt gcgcagaaag gttcggttga tggtggcgga aatttttcca acaaggatgt
     1441 caaataattc atcgttctgt aattgtacca tgatagttga ttgattaatg gttgtctaaa
     1501 caacgatgca aaattactgc cattttcaaa atattgtact cagtttttta atatttaatg
     1561 tattttcaca aaaagtacag gtaatgaaaa ctttgttaac ggtaagaacg gaaagattac
     1621 aggtacataa agtcatcaga gtaatgtagg ctgatgatag taggttaagc aggtcaggac
     1681 aaaccgatga gataatattg tcggaaaact gattttataa aaagaaattc tgaaaacggt
     1741 gggttaatcc gggagagtag ttttatagaa ctccctgaag gtgttttatt attttatcag
     1801 caatctggct gtcggtgccg cttactttga ttttagcctg attgtagaac aactcccttt
     1861 tttgcaacgc tgatgcgata aattcttcca gatcgtcgcc ctgatagctt gcaagcactg
     1921 ggcggtcatt caagttagtg gtccggagtc tggaagcaag ttgttttggt gtcagacaga
     1981 tataaatcgt aacgcccagg gagttcatga tttccatatt gtcaaaaaaa cagggtgcac
     2041 cacctccggt ggaaatgatt gcctgttcgt atccggcaac ttccaggaga cagtttcttt
     2101 caagttcacg gaacctgtct tcacccagtt cgctgaaaat ttctgcaact gatttcccgg
     2161 ctttcctttc gataagttca tccatatccg taaagttcat attcattcgc ttagccacaa
     2221 gccgtccaat ggtggtttta cccgacccca tatatccgat aaggaagatt cgcatgatgt
     2281 ttgtttgatt tcattggaaa cagattgcaa aagtacatat ttttttctgc ccggttacac
     2341 tgtacgtcgg cttgtacaaa ttcctcatga tttttaatgc aataacattg gttgcaaaat
     2401 aagcagtgta attgaaataa tactcatata acattgataa tatattgaca aaatgataca
     2461 ataatattta aaaacaatta tctttgcgtt gaatttactt gcaataattc tatgagcatt
     2521 aaaaattctg tcttattgat attcaccggc ggaaccatca gcatgtctgc caatccggaa
     2581 accggtgcac ttcgtcccgc cgactttgaa tcgatgcaaa agctgatgcc tgaattgggc
     2641 gatctgggta tcgatattca gggcattccc ttgcttccgc tcgtcgattc gtcggatatc
     2701 gggcccgata cctgggaacg gattgccatg actatcaagg acaattacag tctctatgac
     2761 ggatttgtgg tgctgcacgg taccgacaca atgtcttatt cggcctcggc gctgagtttt
     2821 atgctcgagg gtttgtccaa acctgtcatt tttacaggtt cacagcttcc cattggcgta
     2881 ttgcggaccg atgccaaaga aaacctgatt acagccgttg aaattgctgc tgctcacaca
     2941 aacggtcatg ccatggtgcc tgaggtgtgt ttgttttttg aggacaaact tttcagagcg
     3001 aaccgaacca ccaaacagaa ttcagagcat ttcaacgcgt tcagatcgtt caattatcct
     3061 gaacttgccg aagcaggtgt tcatatcaga tatttcccaa attttatccg gaaaccggat
     3121 aaaaatgccg tgctgaaagt tagaacaaaa attgactgta atgtggccat tctgaaaatt
     3181 tatcccggta tcacggagca aacggtgcag ggtgtactca atatcccgca actgaaggcg
     3241 gtagttctcg aaaccttcgg agccggcaat gcgccccgtt tcgactggtt ctaccgcgca
     3301 ttgaaaagtg ccaccaaacg tggaataatc atcgtgaaca aatcgcagtg cagtactggt
     3361 tcagtagaaa tgggcttgta cgaaaccagc ctgaacctgc taaacgcggg ggttatcagc
     3421 ggttacgaca gcaccactga agcgctgatt tccaagctga tgtatctgct gggtgaaaat
     3481 gaagatattg aaaccgtgaa aaaactgatg caaaccagtt tatgcggtga gataacggta
     3541 catccgtaaa tgaaaacaac tcacccctgg cccctaaagg ggaacaaaaa atgcttttaa
     3601 ggaatgtttt aaagcaatat ggcttatact aggatagttt ttgcgaaagg ataaattaca
     3661 cgataataat tgtggggtat aatgcttatt tctaaaaaaa tatcacacct attgtactta
     3721 taaatctttt ctttctctcc cccgtagggg aagttggagg gggcttttac catactacaa
     3781 tttcatccag aaaaacccag gttccctctt tgttgtttgg caagccctga aaacggataa
     3841 actgtccttt cgcattgccg gtccactgaa aattaaagaa aatggttccg gctgttttca
     3901 tgtcataagg attagccatg cgtgcaattt ctgtaaagtt cttattatca tcggagacag
     3961 aaataaccac ttcgtttggg aaataaatcc aggctcccgg attctgcatg aaacgtgcac
     4021 cgattttctt cagtggcgtc acttcctgca tgtcgatggt tacgtccatt ccgtcggtca
     4081 taaatccctg ccagcgacca tccgagtggg tcagaccacc cgttcgtccg tcgataagcg
     4141 atgaactgcc tccggctgga tagtagtgat tgacaggaat attgtaaatg atttttttgc
     4201 caatagcttt gtgataatca aaccttctgc cggttgcttt tcccactgct ttacccctgt
     4261 cataaacctg cgcggtaatg aacgcggagt cgtttacagc aagtggtccc gaatataatg
     4321 ctgaaaaact tatgggtaat gtaccatcga gagtatagcg gatttctttc ccgtattttt
     4381 cagtcgttaa ggttacaagc atttttttgc ctacactatc aatgtcctgc gcaaaactta
     4441 cttcacccga aagggtaaac ggatgatatc cgcggttttg caggaaaggt attgcttcgc
     4501 tcacgcgccg gttgaaatca ttccacgatt tattttcgta ctcagtccag gctacctcgg
     4561 caagtgccag caagcgtgga taaatcataa attcggcctg ttcgggtgtg gagatatatt
     4621 ctgtccacag gttggcttgc acgccggcaa tatgttttgc ttccgcagga gttagacttg
     4681 aggatactgg attgtagctg taaacttttt cgaggggcag gtaaccgccg attgcctctg
     4741 tctgatttga agggtcggtc tgataggaat cgaagtagca gtaagcaccc ggagtcatga
     4801 ttgcatcgtg tccgctcttc acggcttcaa tgcctccgct ttcgccccgc cacgacatca
     4861 ctgtggcatc gggagccagt ccgccctgca ggatttcgtc ccagccgatg agtttgcgtc
     4921 cgtgtgccgt gaggaatttc tcaatcctgc gaataagata actctgaagt tcatccacat
     4981 cattaatttt ttcatctttc attctcttct gacatttcgg acaggttttc cagggctcct
     5041 tattggcctc gtccccaccg atatgaatgt attcagaggg gaaaacagcc atcacctcgg
     5101 tcagaacatt ttcaagaaaa ctgaatgttt cctcattacc cgggcagaac tcactgtttt
     5161 tataggggac acccgagcag gatagatgcg gataaacagc cagcacttcc tccgaatgtc
     5221 cgggcatttc tatttcgggg ataacggtaa tatgtctttc ggcggcataa cggacgattt
     5281 cacgggcttc gtcctgtgta taatagccgc caaatgcgcc ggtgtctgtt ttactgacat
     5341 agtgacgcgg tgatgcccac cagtctttcc aggttttgtg tgttcgccag gcagctaaat
     5401 cggtgaggag cggatatttt tttatttcga gccgccatcc gggaccatcg gtcaagtgcc
     5461 agtgaaagcg gttcagtttg tagtatgcca tcatatccag ctgctttttg ataaaatcca
     5521 cagaaaaaaa atggcgggac acatcaagat gcattccccg gtatgcaaac cgcggactgt
     5581 cggtgatgtt cagggcagga atttgtttgg ttgcaccgta ttcaaccgtc agttgcagta
     5641 aagtctgcaa gccgtaaaac aatccttttt catttccggc agtaatgctt attttgcccg
     5701 gttcgatgcg gagccggtaa gcttcatcgc tgacgaaatg tgctttcctc ttctctgtga
     5761 atagctcaat taaggcttta tgtccttttc cggacctgaa acctgctttg ctgaggtaat
     5821 ctttaaaatg ctttgcagat tgtcgcggaa ttccgctggt ccttatctgc accgcttgag
     5881 tgagatcaaa gtttccactc agctgttcaa cctggttcgg ttttgggata accggcagat
     5941 caacagccat aacgggaaaa aacatcccga tacaaaagag gagtattata tttcggacgg
     6001 tctgtttcat ggtttgcagg tatattcttc cgacagggaa attcacgata atatgtttag
     6061 tcgggggcgg ggagtgcttt gcttttattt cagcgaattc caaccctgag ctttgagggc
     6121 gatttcttcg ccttctcttc ctaccagaca gagtccgagt tccggtttgg tgatgtgacc
     6181 gatgatgccg ataccttcca tagtcactat tttatcataa tcatcgagcg aaacggtaaa
     6241 cagaagttcg taatcctcgc ctccgttgag tgcggcggta accaggttca tgttcagttc
     6301 ttcggccatg acagctgcct cataatttat aggaatctta tcttcgtaga tgcggcatcc
     6361 ggtgttgctc tggctgcaga tatggatcag ttcggaggat agcccgtcgg aaatgtccat
     6421 catggcagtg ggtacaattc cttttttgtg cagagcatcc acaatatctt tccgtgcttc
     6481 aggcttcagc tgacgctgca ggatgtattc tttgccctcg aaatcgggct gtacgttttc
     6541 ctgctgaccg gagaatacca ctttttcgcg ttcgagcaac tgcaaaccca tgtatgcgga
     6601 acccagatcg ccgctaacgc aaatcaggtc gttcggtttt gcgccgttgc ggtacacaat
     6661 tttatccttt tcaccttcac cgatgcaggt aatgcttata atcaggccgg tgagggaagc
     6721 ggaggtatcg ccgcccacca gatcgacgcc gtatttttcg caggccagat taattcccgc
     6781 gtatatttct tccagatctt ccaccgtaaa acgtttcgat acacccagcg ataccgtcat
     6841 ctgtttgggc tgtccgttca tggcatagat atcggagaaa ttgaccacag ccgatttgta
     6901 gcccagatgc ttcagcggcg tataggtcag gtcgaaatga atcccttcca gcagcaaatc
     6961 ggtggtaaca agcacctgtt tgtctttgta atccagtacg gcggcatcgt ctcctacgcc
     7021 tttgactgat gacggattct gtaattttat tttttcggta aggtgtttga tcaatccaaa
     7081 ttcaccatat tctgatattt cggttctgtt catagttgtc cttttataca ccccgaatgg
     7141 aggtgaaagt atgtatttta attataaaac cggaaacgtc cgggtgctat ggtttatttt
     7201 acgggtatct tatcaattct tcgttggtgt ctgccacctt cgaaatcggt ggagaaaaag
     7261 gcatcgatca ttttcagcgc ttgttcttcg gatataaacc gggcgggaag cgaaatgatg
     7321 ttcgcgttgt tgtgaagccg ccccaataca gcaatttctt cattccagca taacgcggca
     7381 cgtattccct gatgtttgtt taacaccata cttatgccat ttccggtacc acaaattgaa
     7441 attccgtaat caaacaatcc gttttcgatt gctgtagcca tcgggtgagc atagtcgggg
     7501 tagtcacagc tttcggggct gtcgcatccg aagtctttca gtaaagccgg agctttttcg
     7561 gtgatatatt tcaggataaa tgtctttagc tggtaaccgg cgtgatcgtt gcagattgct
     7621 attcggagtt cgttgaaggg cttcataatg aattgtttag tttgcacggg tattattctg
     7681 aagtgaaata atcagcctgc gcggcctgtt tcaaaactta tttgcaaagg ttgtgaaaaa
     7741 tcagcaatct gccaacaaag cgccctttat ttttgttatt aaaatcacta aacagcatta
     7801 tattaacaaa ttatataaaa ctattttcca tgaaaaactt cagccggata tcattcattt
     7861 tgctgataat cgttacggcg ataggcgctc cggcctgcaa caagtccaaa acgacagcgc
     7921 aaaacagccc gacaaacgac acattgccaa ggattgtgtc agataaaatg cggtttaccg
     7981 agagtactta cccttaccgc gatggattta tcgtggccaa tttcggctca tcggagttaa
     8041 atcccctgaa cctcgagggc aaaggctatc tgctgtttct gaaagaaacc acggtgacga
     8101 tgcttatccc gcccgacgga aatctgaacg cgccccgcgg catgtacgaa aggaatggct
     8161 ggctgtttat agccgatgtc ggcaaaatag tggtgtataa cctgaactcg ctgaacgtga
     8221 aaccgcaaac cgtttggttc cctaaagaag acgtgatggt caacgatctt gctgctcacg
     8281 gcgatacctt gttcgcgacc gtcagcaata ccggaagaat atttacagta aatattaaag
     8341 accccgcccg tgtcagtcgg gagaaaccgg tgaaatacat caacattccc ggtccgaacg
     8401 gcattgctgt ggctttcggc aaactgtaca tctgttcgtt ttcgcaacag ggcaaaccgg
     8461 atgcagcgaa tgtggtgtat gtggtcaatg acctgaaaaa gcctgtaccg gaaaaaataa
     8521 tcacacaacc gggcaactac gacgggattg ctgtttcgga cggtggcacc aaactctatg
     8581 taagcaactg gtcgccggca ggagtgatcg aaattgattt atcgactaag gccgtgaaaa
     8641 agattaatct gccggtggaa gtaacaggtc ccgccgattt ttcgctttgg gacggaaagc
     8701 tgataatccc cgatctggtg aagagccggg taataatgat gaaaatgcca taaagccccc
     8761 taatccccct tgggggactt tggctgtttc gtttttatca gaatatgctc aactaattgc
     8821 aaacaaattt caggacgata gatactgaat aatcctgaat ggtgtttact tttttgtaaa
     8881 acctatttag cataagcgag ggcgtcactc caggtgacgc cctcgctaaa tatggacaat
     8941 gtcaggtttt tgtcttgttt atgaaaacat ttctacatct tcttttgtaa tcttatttcc
     9001 tcccaagatg atgagtcgtt ccaccacgtt gcggagttcg cggatgtttc cggtccagga
     9061 tcgttcctga agagctttca ccgctttggt atcgaaagtt ttcagctgga tgccctgctc
     9121 agcgcaaatc agttcgcaga aatgagtgac cagcagcgga atatcgtcct tgcgttcgtc
     9181 cagggtgggg acgtggattg gtattacatt caggcgatgg aaaaggtctt cgcggaagtt
     9241 tcccttttca atttcggctt tgaggtcttt gttggtggca gcgatcacgc gcacattgac
     9301 tttgatgttc ttatcgctgc ccacgcgtga aatttcattt tcctgcaggg cgcgcagcac
     9361 tttggtttgt gccgaaaggc tcatgtcccc gatttcgtcg aggaagatgg tgccgttgtc
     9421 ggcctgttca aacttaccga tacgctgttt cacggccgaa gtgaacgaac ctttttcatg
     9481 tccgaaaagt tcgctttcaa tcagttccga aggtatggcg gcgcagttca cctctacata
     9541 gggtccctgt acgcggttgc tgtattcata caaaagccgt gctacaattt ctttaccggt
     9601 accgttcgcg ccggtgataa gcacacgggc atcggtggga gcaactttgt gtatcatgtc
     9661 acgcactctt tcgatgcatg gcgtatttcc caccatctgg agtttcttgg ctactttctt
     9721 tttcagcgtt ttggtttcgg tcaccaggtt gtttttctcg agcgcgttcc gcacggtgat
     9781 gagcagacgg ttgaggtcga tgggtttttc gataaaatca aaagcgcctt tttttatgca
     9841 gtcaactgct gtttctatat ttccatgacc cgaaatcatg accacgggcg cttccacatc
     9901 tttttccttc agctgttcga gcagttcgat gccatccatc tcgggcattt ttatatccga
     9961 aaaaatgatg tcgaaaagat cattcagagc cgcatccagt ccctgtttcc cgttttcggc
    10021 caggattacc ttatgttttt cgaattccag aatatctttc agcgtattcc ggatgctgcg
    10081 ttcgtcgtca ataaccaata ttttagccat aaattaaaac aattatagat agaaaaatag
    10141 aaataagtaa gaaagtaatt ctgattcaga aagggccagt ttttagatat caaatccgat
    10201 atctctccgg aagtattttt tgtcgaaatc aaccaattgg gcatttctga aagactgcgc
    10261 cagtgcttcg tccttgtttg tcccataaga acttatggcg attacacgtc cgccgttggt
    10321 gagaatattt ccgtcttttt cggtggtacc ggcgtgaaat gcaatgctgt ctttcacatc
    10381 ttcaagccct gtaatcactt ttcctttttc gtaaacatcg ggataaccac cggaaacgag
    10441 catcactgtc accgctgaac gttcatcgaa ttcaatcgtt ttttcatcca gattaccagc
    10501 tgcaacacct tcgagcaggt caaccagatc cgatttaagt ctcaacatta caacttctgt
    10561 ttcggggtcg cccatccggg cattgtattc tatcaccatc ggttcgttat caactttaat
    10621 cagtccgaag aaaataaaac ccttgtacgt aatgccatca gcctgtagcc cttcgacggt
    10681 tggacggatg atgcgttcct caactttctg catataggtt tcatcggcaa aacttaccgg
    10741 cgacacagcg cccattcctc cggtgttcag tccggtatcg ccttcgccga tgcgtttata
    10801 atctttggct tccggcagaa tcttgtagtt tttcccgtcg gtgatgacaa aaaccgagca
    10861 ttcgatacct gaaaggaatt cttcaatgac taccgtttga ctggctttcc cgaatttgcc
    10921 ttcgagcatg gatttaagtt cgtctatggc ttcgttcgtg ttgttgatta tcaacactcc
    10981 cttacctgct gccagaccgt cggctttcag tacgtaggga gctttaagtt cgtgcagaaa
    11041 gtcgataccg gcattaatat tttcagcggt aacacttaaa tagcgggcag tagggatgtt
    11101 gtggcgatgc atgaattgtt tcgaaaaatc cttgctgcct tccagttgcg ctgcaacgcg
    11161 gttaggtccg attatcggaa tggtattcag atctccgtcg gcttcaaaaa agtcggcaat
    11221 tccgtttacc agcggttctt ccggacctac taccaccagg ttgatgttat attcaagcac
    11281 tgtctttttt acggcctgaa aatcggagat attcacaggg atattttcgc ccgcctgttt
    11341 tgttccggca tttcccgggg caatataaag tttggtgagt ttcgggcttt gcgccatttt
    11401 ccaggcaagg gcatgttccc tgcctcctga tcctaaaagt aatatattca tcgttttatt
    11461 ctgtttattt tcttttcatt catttatatc attcttccca gaccaggtaa gctgatacca
    11521 gccagtgatt tagtttgtta tcttcgattg cctgcgcgtt gggtatgctg attgtgaggg
    11581 tgtgttcacc actccgtata tcgattagcg gtacaactac tggaggtaca tcgcttcccg
    11641 gacaccagtt ggaacgcgaa agatcggaag aggcaatcgg ttcttctatt tccttttcgg
    11701 ctctgccttc atcgctgatg taagcaattc tgcgtttgcg cagccacacc cccgatgtgg
    11761 gattgaagcg gcgaaaggaa gcacaatcgt tgcgccaggg tataaaacgg tgaacgacag
    11821 cgccgtcaac cgacaggatg ttttctttct gtacaaattc atcaccgccg gaatggcctc
    11881 cgtgacccgt tacgatgtat ttcaaccgga cgtttttagc tttccgcggc agactgaaag
    11941 gaacggtaat atcctttctg gcaaatatat cggggtaagt ctgaccaagg taataaaccg
    12001 tattgattag tggcattact ttacggatgg gcatcacatc ctgtttgatt ttgctttcct
    12061 caacatttat ttcgaaattt actacataac ctttttcagt ccaggtatcg atataaatac
    12121 ccacgtaaac ttcacctttc agcagaggat ataaatcggt gatatcctgt tgccaggcgg
    12181 cttcctgtgc ccagccatca atatacaccg gtcgccgttt gtatgatgtc gaatcttttt
    12241 cggcactgaa atgtcccacg ccaaaaggag tcataaaccg catcaattcc actgtgggta
    12301 aataatcttt tccgactatg gtgccaacca gtttttcgag ttttgtggag tcaatatccg
    12361 gatatttcgc tgtgccgcgt gctacggtta tcatgttaat gagcgactct ttcgggatga
    12421 taaaacagga gccggattta tcccagggat cgccgttgga ggtgagtctg agtttggctg
    12481 tgatttgcac attgcgtttg tattccggca atacaatttt ctttatagcg attcgtccgt
    12541 ttgccagccg gatgaatcct tcggcttcgt tcacaccgtc aggatacttc gcgggattaa
    12601 actgaatatt cagatcagta aacagatttg tttccagatc gccgcaaata ggggctgttt
    12661 ttgccatggt gacctgaatc ccaaagagca ggatggagaa aaaaaacagt cgtttcatgg
    12721 gtttgtagtt ttggaatatt cggcaatttc ctcagagagt atgtgttcca gttcttcagg
    12781 tttgccgttg atggtaagtt tgccatttat tgcccaggat atatgtccgg tttcttcgga
    12841 aatgacgatg gcaatggcat cggatatttc cgagataccg agtgccgacc ggtgccgaag
    12901 tccgagtcgt ttgggaatgc tctgattgcg ggatacgggc agaatgcatg ctgctgcttt
    12961 cagtcgccgg ttggagataa tcagggctcc gtcgtgcaac ggactgtttt tgaagaagat
    13021 attttcaatc aaccgcgagg tgatgcgcga gtctattatt tcgcccgatt gcgtgtagaa
    13081 ttccagatcg ttgctgcgtg tgatgataat caggcctccg gtggccgttt tcgaaagatt
    13141 gcggcaagct attacaattt gtatcaagtc gtagtcggtc cggatttgtt cgttctcggt
    13201 acctttcaga aatttcttga agtagttcag aattccccag tttttccggg acccgagtct
    13261 ggaaaagaac cgccgtattt cttcctgaaa aagcacaatc agcgcaaagg caccaacgtt
    13321 taccagtccg tcgagaattt cacccagcaa ttccatgcgc agcaccttgg aaaccacaaa
    13381 ccagatgatg acaaaggtca tgatgcccag aaaaacattc agcgcgcctg agcgtttcag
    13441 cagtctgaag gctccgtaaa gcagaaatgc tgttaagaat atatcaatga tgtctttaat
    13501 accgatatta aaagccatat tgtttggttt ttatgaaann nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnntc aatgatgtct ttaataccga tattaaaagc catattgttt
    13681 ggtttttatg aaagcataag atgaataaat ctaaactata aagtaaatga ttactgcgga
    13741 atgtcatcaa accctgaaaa tcagctgttg ttatatttct caaataattt aacagcttcg
    13801 accgcttctt tcacatcgtg aacccggaga atatccgctc cattcatcag ccccagcata
    13861 ttgagcgccg ttgtaccgtt gagtgcttct tcgggagtat ttcccagcag gttgtaaatc
    13921 attgattttc tcgaaatgcc aaataaaaca gggagattca gttctttgta atagctcatt
    13981 ttttttagca attcatagtt ttgatctagc gttttcgcga aaccaaatcc ggggtcaatg
    14041 atgatatctt tcactcccag atgaacaagc tgtgccactt ttttcccaaa ataaaaaagc
    14101 atttcagaca tcatgttttc gtatttcgtc agttgctgca ttgtctgggg agtgccccgg
    14161 gtgtgagtga gtatgtatgc tgtattcaaa accgctactg tctcgaacat catgtcgtcc
    14221 attgttccgc cgctgatgtc gttaatgata tctacgtgac attcttcaac tccccaccgc
    14281 gccacacctg agcggaaggt atcgagcgaa atgacgatgt ccggaaaatg ttttcgaatg
    14341 gctttgaggg gcaatcgaag ccgttccagt tcttcttctt cggtaatgtg agctgctgag
    14401 gggcgcgtgg agtaagctcc tacatcaagt attgaagcac cttcggcgaa tgctttttca
    14461 acacggttca atacctctct ctccgaaaat aacctgcttc cgctgaaaaa agaatcgggt
    14521 gtcaggttgg caattgccat tacagcaggt tttgacaagt cgaataaccg gccattcaga
    14581 gttaactgga aaggaatttt attcattcgt ataaaattta ttcacgttag ctttaggcat
    14641 taaaagtgtt tatttctcca caaaagtaaa aaaataaatg cttttatgct tcgttttaac
    14701 agttttatta ataattttac tccaaaatat acgataagta ccgaaaatga gttatataca
    14761 gtacagaaat caaaacaatt aattttttta gcataaaagg ctcattcata aaaaaaagta
    14821 ctaattttgc agactatcat aagctaaaat taattaacaa tttaccattg ttttatatga
    14881 aacgagcatg aacgcagcgg ttcaccaaag ttagtgaaag tccggcatgc gagttctccc
    14941 gataaaccgg gacaagctat gtgaccttta aaaacaatta tttacatatg aaattgatta
    15001 acgttgctaa actactgaca ttcacttttg ccacactggt tattgtttca tgcggttcaa
    15061 aatcaggcgc cagtgccggt tttggatcgg gcaaaaaatc ggcaatatcc aataccacag
    15121 ggtggagata caatgatgaa aaaatgaccg gctttggagt gaaaaagaac tacaaagctc
    15181 aggtgccaac cggtatgatt gccatcgaag gaggtacatt taccattggt gaaaaaggag
    15241 aagtaatcac cggtgaacgt aacaacctgc gccgtcgcat cacggtgatg tcattctaca
    15301 tggaccagta cgaaatcagt aatgtaaact ggcgtgaata tacccactgg atgaaaaccg
    15361 tgtttcaaca gacagctccg caactggtag aaagagcaca gcctaatggc gaagtatggc
    15421 gtgaaccgct tgcatataac gagccacttc ttgaacacta ttttactcac cccgcttttg
    15481 atgaatatcc cattgtaggt gtaacatggg aacaggctat ggattactgt caatggcgta
    15541 cagaccgtgt aaacgagctg gcgctgatta acgccggtgt cattgaagcg cccgattttt
    15601 catcgcttaa gaaaattacg aatgccgact ctattgccaa taattttgtg ttcaatactc
    15661 agaaatatat ttttcacagc aaatacaatc cgaaaccagg aaaaaaatcg ctgaaggata
    15721 tttacggaca aacccgtaaa gccgacatgt cggacggaat catgttttcg aattacagac
    15781 tcccgactga agccgaatgg gaatttgctg catacgccat aaaatcagat tcgaaagacg
    15841 gtttggtgaa agaagggaaa gtttatccct ggaacggaag tcagttacgc tacaataaaa
    15901 aaggaaaata ccttggagaa atgcaggcta acttcgttcg cggacgcggc gacatgatgg
    15961 gaacttcagg aagcctgaac gataaggctg ccataactaa tgcggtaaac tcatacttac
    16021 cgaatgattt tggtctgtac aacatggcgg gtaacgtcaa cgaatgggtg atggacgttt
    16081 atcgttcaac cactttcgac gaaatggctg aatataactc tttccgtgga aatgaatacc
    16141 gcacttatct ggccactgca aaagatgccc tcggcaacta tacactgaaa atcgactcgc
    16201 tgggacgcgt ggcaaccgga gttttaccgg gtgatgataa acgcagtttt catgacggtg
    16261 atgaaacaac caaaatcaac accgatttcc cgctgattac cgaacctgaa ggtctcgaag
    16321 cattagccaa taacgaaaaa ctcgatccaa cggatattct tgctccgaaa atatcagaca
    16381 gaacacgtgt ttacaaaggt ggttcgttca aagaccgtgc ttactggttg aatccatcta
    16441 cacgaagagc tttggatcag gacaaatctg ctaacgacat tggttttcgt tgcgccatga
    16501 gtatgattgg tgatataagg agtcagtcaa cgaaaaaaaa ataaaatgaa attatagagt
    16561 attgcaaaca aaaagcatct gtcctcccga acaggtgctt tttgtttgtt tttttaagct
    16621 ttattgaact aaaagtatta cttttgcatt tcaataacta tgacttaact aacacaaaat
    16681 tacaattata tatttatgca gagaacaaaa attgttgatg ttctgaaaag aactgactat
    16741 ggagtagatg taaacattaa aggctgggtt agaacccgtc ggggtaataa aaatgttagc
    16801 tttatcgccc tgaatgacgg ttcaacgata cataatattc agattgttgt tgatctggca
    16861 aaactggatg aagaaaaact gaaaactgta accaccggtg cctgtatcag cgtaaccggt
    16921 aagctcgtcg aatcgcaggg taaaggtcag acagtcgaaa ttcaggctga tgacatactt
    16981 atttacggag gagctgatcc ggaaacctat cctctgcaga aaaaaggaca ttcactggag
    17041 tttttacgcg aaatagccca tttacgcccg cgtaccaata cctttggggc tgttttccgc
    17101 gttcgtcatc atatggctat agctattcat aaattctttc acgaccgcgg atttttctat
    17161 ttccacactc cacttattac cgcttcggat gccgaaggcg ccggacaaat gtttcaggta
    17221 accacgcttg atcttgaaaa catcaaactg acggaagaag gcaaggtaga ttacagtgac
    17281 gatttcttcg gaaaaccggc aaacctgacc gtatccggac agcttgaggg cgaacttgcc
    17341 gctatggcac tgggagctat ttatacattc ggacctactt tccgtgctga gaattcaaac
    17401 actcctcgtc acctttccga attctggatg attgaacccg aagtggcatt caacgaaata
    17461 gaagaaaata tgcaactggc gcaggatttt atccagtact gtatccagtg ggcgcttgac
    17521 aactgtatgg acgatctcaa attcctctcc gaaatgtacg ataaggaact gattgaacgg
    17581 ttgcgttttg ttgttgataa tgattttgta cgtctgccat ataccgaagg aataaaaatt
    17641 ctggaagaag ctgtaagcaa aggatataaa ttcgaatatc cggtgggctg gggaacagat
    17701 cttcagtccg agcacgaacg ttatctggta gagcagcatt tcaaaaaacc ggttatcctg
    17761 accgattatc cgcaagaaat caaagcattc tacatgaagc aaaatgatga tggtaaaacc
    17821 gttcgggcaa tggatgtact tttccccaaa atcggggaga tcatcggcgg ttcgcaacgt
    17881 gaagaaaatt tcgaaaagct cagaacacgt gcagcttcca tgggtgttcc cgaaaaagac
    17941 atctggtggt atctggatac acgccggttt ggtaccgcgc ctcactccgg tttcgggctg
    18001 ggatttgaac ggttgttgct gttcgtgacg ggtatgacca atatccgcga tgtgattcct
    18061 ttcccgcgta ccccgaaaaa tgcagatttt taattaaact gactattcct caaaattctg
    18121 aagataatta aatcatggaa aaaatcaaag taaaagaatt ggaccaggtt gtcgttcggt
    18181 ttgccggaga ttccggtgat ggcatgcagt tgaccggatc cattttttcg aacatttctg
    18241 ccatcattgg taacgagatt tctacattcc ccgattatcc tgccgagatt cgtgctcccc
    18301 agggtacagt aggaggtgtt tccggttttc aggtccacat tggatccggc aaagtatata
    18361 ctcccggcga tgatgccgat gtgcttgttg ctatgaatcc ggcagctctc aaagtgaatg
    18421 ctaaacatgt gaaaaaaggc ggtgtaatca ttttcgatca ggattctttc gaaaaaaatg
    18481 acctggtaaa agccggattt gtctccgacg atccgtttac cgaaatgtca gtgtctgaaa
    18541 caatcagcct ggtgcccgct cagctcacca cccttacaca gaaaagtctg gaaggatttc
    18601 cattggacaa taaagcgata cttcgcagta aaaatatgtt tgctctgggt ttaatttgct
    18661 ggttgttcaa ccggccgatg gaacaggctg ttcattttct gggaaataaa tttggaaaaa
    18721 aaccttcggt tctcgaagca aatgtaaaag tgctgactga cggatttaac tatggtaata
    18781 atttacattt gtccgttcca acatatacca tcgagaagtc tgacgatgtt cctcatggga
    18841 aatataccag tatttcgggt aatacagcta ccgcatacgg atttattgca gctgccgaaa
    18901 aagcaggact ggaattgttt ctgggaagct atcccatcac tcccgcgacg gatatcatgc
    18961 acgaactggc tttgcgccgc gatttgggtg cgaaagtagt acaatgcgaa gatgaaattg
    19021 ccggtgtgac cactactatt ggagctgcat ttgccggctg tctggctgtg accaacactt
    19081 ccggccccgg cttggctttg aaatcagaag gtatcaatct cgctgtgatg gccgaattgc
    19141 ctatagtcat tgtagatgtt atgcgtagcg ggccctcaac cggtatgcca acaaaaaccg
    19201 aacaaacaga tttgctgcag gcattgtacg gccgtaatgg tgaaagcccg ctggttgtaa
    19261 tggctccggg aacacccgat gactgtttcc aatatgcata tatggcttcc aaaattgccc
    19321 ttgaatatat gacaccggtt attttgctga ctgatgcatt tctgggtaac gggacttcac
    19381 tttggaactt gcctaagtta gcagatatgc ctgaaataaa gccaaattac gttcaaccag
    19441 gcatggaagg ctgtaaagtt acattcagaa acgaagaaac aaaagtacgt tattgggcta
    19501 ttcctggaac tcctggcttc gagcaccgca atggtggttt ggaaaaggac tacgaaaaag
    19561 gaagcatctc tactgatggt gaaaatcacc agaaaatggt ggaaacacgt gcccaaaaag
    19621 tggaaaatgt agccaacagt attcccgaca tcaccactta tggtagcgat gatgctgaac
    19681 tgctggtaat tggctggggt ggaacccgcg gacacctcat gagtgcagtt tccgatatga
    19741 atgaaaaagg ggaaaaaata gcccttgcgc atttcaacta catcaatcca ctgccaaaaa
    19801 atacagcaga attgatttcg aaatacaaaa aagtcgttgt ttgtgaactt aacaatggcc
    19861 agtttgcgac tttcctccgc tcaaaagtac ccggagtgaa cttcctgcaa tataataagg
    19921 tacagggtca gccatttacc gttgccgaac tcaaagaaca atttgaatta attctgaaat
    19981 aattttgaag ctatggaaga aataaaacaa tataccccaa aagattttaa gagcgaccag
    20041 tatgttcgct ggtgtcccgg ttgcggcgac tacgccatcc tcaacagctt gcagaaagtg
    20101 atggccgaca tgggtgttgc acctcaggat attgcagtaa tttcaggtat cggctgttca
    20161 tcacggttgc cgtattatat caacagttat ggctttcaca ccatccacgg tcgcgcggca
    20221 gcaattgcca ccggagttaa gatcgcaaat ccgaaactaa cagtttggca gatttcgggt
    20281 gacggagatg gactggctat tggcggtaat cactttattc acagtgttcg ccgtaatatt
    20341 gatataaatg tattgttgtt caacaaccag atttatggtc tgacaaaagg gcaattttca
    20401 cccacaacaa agaagggaat ggttacaaaa tcatcacctt acggcaccat tgagcgtcct
    20461 ttccgtccgg ctgaacttac atttggcgct cgaggaactt tttttgcacg tctgctggat
    20521 gtcgatttga aaaatgctca ggaaactata tcggcagctg cacgtcataa aggcacatca
    20581 atagtcgaat gtcttgtaaa ttgtgtgatt ttcaataacg gagcacacgg ttggattgcc
    20641 gagaaagaaa cccgtcccga caggattata tttctggaac acggtaagcc gatgatttat
    20701 ggtgtaaacc gcgataaagg attaatgctt gacggatttg agctgaaagc tgtaacgata
    20761 ggtgaatacg gaataacaga aaaagattta ttggtacacg atgctcattg tgaagatatt
    20821 acattgcaat ataagctggc tatgatggaa ggacccgaaa tgccaattgc tatgggtgtt
    20881 attcgggatg ttcagaccga aacatatgac caggcaatgg aaaatcagat tgctgaggtt
    20941 aaggaaaaat caaaaataaa gtgttttgac gatttggttg gttcgctcga acagtgggaa
    21001 atttagattc aacacgtatt tataatacaa agagccggat ttgtccggct ttttttttat
    21061 agtataaact gaatttcctt gaaataatac tccttcagat catccgattc ggttcgtaat
    21121 atcaactttc cgtccggcaa tatattttcg gtttccgcca gaaatattgt ttttgtttca
    21181 gtttcgaggt atctgtggta tccgtttttt cgaaacagac tatgaaggta ttcccgatga
    21241 ataaattcag gagcagaatg agtgtacaga tccattatct cagcccggat gagcatcatt
    21301 aattcttccc ggttggtgtc aatgttggta atctgcttta atgaaacggg attgggagca
    21361 tcgctgaaga attcttcctg attgacattg agaccgatgc caattacgca cgtgttgata
    21421 caatctctta tcagcgaatt ttcaatcagg ataccaccga tttttttttc tttccagtag
    21481 atgtcattgg gccacttgat ggatatatct ctggtgaatt tatccagcac ttttttgaca
    21541 cccagtcctg tgatttgtga gagtataaac tgtttatcgg ctttgatcga acggggatat
    21601 agtaataggc tgaacaacag gtttttgttt ggttcgcttt cccaggaatt tccaacctga
    21661 cctttccctg ctgtctgaaa gtctgtgtaa acaatataac cctcgggtaa atcttcctgc
    21721 cgcatctttt gcttaagcaa caggttggtt gatgtggttg atgctatata aaacgctgtt
    21781 gatttcattt gcatactgac agtaaaaact cttcgatttt ttttgtaacc gcgctgagcg
    21841 ttccgttcgg attgttgacc atgatggaaa aaacgtaatt tttattatcc aaagttatat
    21901 aaccggcata acacttcacg cgatggatgg tgccgctttt tgcctggatt tttccctcta
    21961 aagctgtgct tttcaaaaat gatttcaggg tgccattggt gcctgctacc ggtaatgaag
    22021 cccggaaatc ctgactgtaa gccgatttgt ccatatattc cagcaaacct gccataaaac
    22081 ccgaagaaac ggcattgaca ggtgaaagtc cggaaccgtc gtatatgtac aactgatcga
    22141 caggtagtga ccttgatgcc cagaattttt taatgactgt tacagaacct ttgcttgttg
    22201 caacagtgtc atttatcagt gaaaggtaac gaaaaacatg ctctgcataa tgattgttgc
    22261 tctgaacatt gatttcgcga ataatatcac gtaactgagg tgaataatgg gtgtaaatga
    22321 cgtttctctg tttgtttcga acttcggttt cggtggctgt gcgactcact tcgatgcctg
    22381 ccacgcttag tgcttttgta aaatcccgtg caagcagtaa tcccggacgg ggaatatcgc
    22441 ctttaacctt gaatgaggac cggttggctg gaatttcgcc gtagatggtt ctgtggtccg
    22501 aaaaaggggc tccgtagaaa tacgcgctgt cggatctgat tgcggaactt ttcaggttgt
    22561 ttttaaattc aaaaccgggt atttcgggca tcatctttac aatctgaggt gtagtcccaa
    22621 tttcacctga acgaaattcg agtgtgtaca gattgtcttt gtacgaaata ccataagctc
    22681 cagccccgta gtagtttccc atatcttccc agagccattt cggatttact ccttcggtat
    22741 cgaaccgggt tgcgtcggca acgatggcac ctttaatgct ttttattcca gctaattgga
    22801 ttttttttac ccattcgaca agaaatgcag tatcgcccag cattgcggaa ccaagtgtgg
    22861 gatcgccact gccgtgtatg tacagatttc cgttgagagt gccgtctgga cagataggac
    22921 tgtcggtttc gagggttgtt tcaaagcgaa attcaggtcc gagcaattcc agtgccgtag
    22981 cggtggtaat cagtttttgc gtagaagccg gaacaacagg attattggat cgaaaacttt
    23041 cgacggtttt atttgtattc agttttttta cggtgatact gatattggca ttccggagca
    23101 aatcgttatt aataaaatgc ttaacaggat tggatgccgt aaccgggatt atagctattg
    23161 ccaaaaagag tatcaggatt tttgatttca tcggaatgga tttaataaat taatatttcg
    23221 gtctggatgc aaaagtactt gaaaagtatc tattcagaaa aaataagaaa tcaattctcc
    23281 ggtttttatc tgtttttatg tatttttgtc agtagaaaat caaaaaaaat gacgggaaat
    23341 gaaagaaatc aacgagttgg cgctggatga ttattctgtt tattacgaaa aaaccgaata
    23401 tcctgaaaat cccgaagaac ctgttttgat ttttttgcac gactcgtggg gatgtgtcgg
    23461 gatgtggggt gactttcctg agaaattgat gaagattgcg ggcttgaatg cttttgtata
    23521 tgatcgaagg ggttatggtc gttcatcacc cttcggtact gcacaacgcg acaaaaatta
    23581 cctccaccaa gaagccggtg agcttatccg ggtgatggac aaggcaaata tccgaaaggc
    23641 ggttttgtat ggccatagcg atggtgcaac cattgcattg attgctgcgg caaaatatcc
    23701 cgaacgaatt gttgcagtca tagccgaaag tacgcatact tttatcgaag agaaaggaaa
    23761 aacggcggta ttggaaagtc gcgaaagagc aaaacataac tcattgcttc aaactctcga
    23821 aaagtatcat ggcgacaaaa ccggagaact cttcaggcgc tggcatgaaa cctggctgag
    23881 tgataacttt gccgactgga gtattattcc attactctgt gatattcaat gcccggtgct
    23941 ggcatttcgt ggcaaggacg atccttacga ttccatagaa cagcaaaacg tactgaagag
    24001 tgaaatgtca tcaccgttga cgacagctgt catacccgat gcgggacatt ctccgagaat
    24061 tgataacgaa acggtgacga tgaaactgat aaaatctttt cttagttgta atagttttga
    24121 ataatatcag atatacttat ttcagaataa aataaattaa tatgggacta ttttcagcac
    24181 taatgggtaa tgcgggaacc attgatcctg caaaaatcga aaaagaattt ggcaaactac
    24241 tgaccgaaga tgaagttatc gaaatgggtt tcaaacttat tcgcgatacg tttattttta
    24301 ccagtaaacg attgattatg attaatgttc agggaattac cggtagcaaa caggaatatt
    24361 tatctgttcc ctataaaagt atttcaaagt tcagtatcga gtctgcaggt ccttttgatc
    24421 tggatgccga gctgaagata tggatttcga gcgagcagaa tccgagtgtc acccgaaact
    24481 tcaacaaatc ggtcaatgtt tacgacgttc agaagatttt agctcattat gtgttgaaat
    24541 aaattagaaa tatggccttt attactctgc aaaacatgaa atttcatgct ttccatggtt
    24601 gtctggagca tgaaaagaag ttgggtaaca cgtttcttgt aaccttgcgc atggagcttg
    24661 atacctctat tgccgcggaa acggataacc tggatgatac tctgaattac cagctcgtgt
    24721 atgatgtggt gaagcaagag atggagaaac cttcggacct gattgaacat gccggacaac
    24781 gtattctgga cgctgttttc aacaattttc cgaagattca gtccttacat attcagctta
    24841 ccaaactcaa tccgcccctc ggtggaagtg ttgaaagcgt tattatcgaa ctggataaaa
    24901 agagataaat cattatctga ctgacgtatc cgcttcattc aaaataataa ttgtatattt
    24961 gcattgcatt ataaatcttt aaaaaatgaa cgcaataaat cgggcgttca tttttttgta
    25021 ataaaaaaac taattacaat gtcagaaaac atcaaacaat cacatcccaa agggctgtac
    25081 tttgttttcg ccacaggtat gtcggagcgt ttcagctatt acggaatgcg tgccatattt
    25141 gtgctctatt taattaaagc cctgctgatg gataaggaat ttgcatcagc tatttatggt
    25201 aactacaccg gtctggttta cctcactcca cttatcggtg gttatgtagc tgaccgctac
    25261 ctgggtatgc ggcggtcaat attctggggt gctgtgctga tggttgccgg acagttattt
    25321 atgtttgcca gtgcgcttaa ttaccagagt accgaactag cccggtggct gatgtactgt
    25381 ggtttggccg gacttatttt cgggaatggt tttttcaaac ctaacatttc aaccattgtt
    25441 ggtcagttgt acgaaagagg cgacaaacga ctcgattcag cttataccat tttttatatg
    25501 ggtgtaaatg tgggtgcatt tctgtcgccg cttctttgtg gatttctggg cgatacaggc
    25561 aatcctgccg atttcaaatg gggttttctg gttgcagctg tcatgatggg gctaagtctg
    25621 atattgtata ttactcagaa aaataagtat ttgattggtc cggatggaac agctataggt
    25681 attcatgcgc cccggaaagc agaaaaagca aaggaaaaaa acaacgaact ttcaaaagaa
    25741 aagctgaatg ttaagaaaat gatggttttg gctggcggag ctgtactctt gttttccttg
    25801 tttatggttt tatttgataa agatgtgatc ggagccgtta tttatacagc ctgtatcgta
    25861 gttccggcct atgttattac cgacagttca ctttcaaaaa tagaaaaaca gcgcatctgg
    25921 gtaattttta ttattgcttt ctttgtcatc tttttctggt cagctttcga gcaggcaggt
    25981 gcttcactta cttactttgc cgatgaacaa accgacagaa atatttttgg atgggaaata
    26041 ccagccagct ggtttcagtc attcaatccg attttcgttg ttgcttttgc tcctctcttt
    26101 tcgatgatgt ggcttcgact gggacgtaaa aacatggaac cggcttcacc tacaaagcag
    26161 gcaatcggat tattcctgct ttcgctggga tatttactca ttgcatttgg agtaaaaggg
    26221 gctgcgccgg gtgccaaagt cagtatgctg tggcttacag gtctttattt cattcactcc
    26281 atgggtgagc tggcattgtc gccaatcgga ctttcgatgg ttaataaatt gtcgccggta
    26341 cggtttgctt cgctgatgat gggtgtatgg tatctttcaa tggctactgc caataagttt
    26401 gccggtgtgc tcagcggact gtatcccgaa gccggtaagc caaaatcttt catcgtattt
    26461 cagattacgg atttgtacga tttctttatg gtgtttgtga taatgtcggc aatagcttca
    26521 gtcattcttt ttgcactttc gaaaacatta caaaagctga tgcacggagt gcggtaaatt
    26581 accgccttag catcatcttt gtttaaaaac tcaaaggggt tttaattcaa tgacttcgag
    26641 gtctttgatt ttgtttccgt caatcacgaa ccgtacgatg gtctgaactt tttggaaacc
    26701 atatttcccg gctgaacccg gattgatgtg aagcaactcc agtttcggat cgtatattac
    26761 tttcagtata tgagaatgcc cgcaaatgaa taatttcggt ggattagctt gtatttcagt
    26821 cctgacggat ggatcatatt tacccggata acctccgatg tgtgtcagcc acacatccat
    26881 ttcttcgcat agaaaacggt tgtgtttcgg aaaaatagag cggatgtcct gtccgtcgat
    26941 attgccccaa acgcccctca caggcttgaa cgcctgaagt ttgctgacca cttccattga
    27001 gccccagtcg cctgcatgcc agatttcgtc tgtttctttg aagtagtggt aaatccgttc
    27061 gtccagtagt ccgtgagtat ccgttagtaa tcctattttt ttcatcagca taatctttat
    27121 ccgacaaatg tagctatttt tttccggtca gtctttccga tcttcgacct gacaggatga
    27181 ttccggcaat tttaaggatt ggtaattcac tgaaattcag tatttttgaa atgaataatc
    27241 ttttcattta ttaactatta aaatgctact taaagggtta tattttgcgg atattcttaa
    27301 aaatacctac atttgcaatc ctttttactg atttcagaag cgtgaagaaa cagctcaaac
    27361 atatcatttc agatttcatg cagcggatgc gcttcaagta tcgtgtttcc attcttaatg
    27421 aaaatacact cgaagaaacc tggcataccc gtttgtcgcg attcagtgtc ctgctttttg
    27481 tatcagcttt ttttctgacc acttttattc tgcttacttt tctgattttt atcacaccgt
    27541 tgcggcatta tttgcccggt tatggtgaat cggggaatcg aataaccatt gttcagcaat
    27601 cgatgacaac agattcgctg atgcatcagc tggaattgca acaggcttat ctgagcgttt
    27661 taaaagatat aatcaacgaa aaaccccaga acgacacaat aaaatcactg gattctatca
    27721 tgctcagaga gcgggcaaca gtattgcttg agaaatcaaa gagagaaaac gaatttgtaa
    27781 aagactttga agaaagtcag aaattcaata tcagctctat cagtgcccga tccggtgaaa
    27841 aaatgtatgt attttttcgg cctgttcgag gggtaattgc ttcaaaattt gaacctcagg
    27901 atggcaattt cggggtgtcg gttatcactt cttcgcagga aaatgtaatg agtgtactcg
    27961 aaggaaccgt catctacgcc gcatttactt ttgactttgg atgggtcatt gaaatacagc
    28021 acgaaaacgg gtacatttcg atttataaaa acaatactcg tctcctaaaa agtaccggtg
    28081 atatggtcaa agccggtgaa acaatcgctg ttacgggcga tgaacataat gttaatgaac
    28141 gtaaacattt ctattttgag gtgtggaaac agggaattcc gctaaatcct caggatgtaa
    28201 ttgtttttta gcgtgttgaa ataatgataa tacaactatg actgaaaaga aaaaaaatat
    28261 cgcaatactt ggttctaccg gatccattgg tactcaggcg cttgaggtga tacgtgacaa
    28321 tccggataag tttgaggtat ttgcactcac agctaacaat aatattgatt tgctgataga
    28381 acaggcctgt gagtttcagc ccaacgtggt ggccattgcc aatgaaaaac attatttgca
    28441 acttaaacag gctctcgctg atcagcagat aaaggttttt gcgggtattg atgccatctc
    28501 acaaattgtg gaaatggaac ctgttgatct ggtgctgact gccatggtgg gatatgcggg
    28561 tttgaaacca accattcagg caattaaagc aagaaaaacg atagcactga ccaataaaga
    28621 aacactggtc gttgccggtg aaattattca ggagctggca atccaaaata aaactgccat
    28681 tttgcctgta gattccgaac attcggccat ctttcagtgt ctggtgggtg aaggggataa
    28741 tgcaattgaa aaggtaattc tgactgcttc gggaggaccg ttccgtaaca aaaccattga
    28801 tgaacttaaa aatgtaacgt cgagacaagc attgaagcat cccaactggg atatgggcgc
    28861 taaagtgacc atcgactcgg caagtatgat gaataagggt tttgaggtca ttgaagcccg
    28921 ttggctcttc ggacttaatc ctgctcaaat cgatgtagtg gtgcatccgc aatccattat
    28981 tcattcgatg gttcagttta ccgacggctc agtcaaagcc caactcggta tgcccgatat
    29041 gagactgcca attcagtatg cttttgcgta tcccgacagg attaaatcaa attttccgag
    29101 agtggacttt acaaaataca acgaacttac ctttgaaaaa cccgatatca aacggttcag
    29161 aaatctggcg ctcgcttatc aggctatgga aaatggagga aatatgccct gtatcctaaa
    29221 tgccgctaac gaaatagttg ttgcagcatt tctgaaagat gaaattggtt ttttggaaat
    29281 gagtgacatc atcgaaaacg cgatgcaaaa agcaactttt ataaaaaaac cgacctatga
    29341 cgattacgta atgacggatg cacaagtacg tgaacttaca ttaaaagaaa taaaaaactg
    29401 aatttgaaat aaaaaactga taattaagaa atacattaat acaatttatg gaaacatttt
    29461 tgattagagc cttacaatta atcctgagtt tgtcaatact ggtcgttttg catgagttcg
    29521 gacatttcgt tttctccaga ttatttaaag tgagggtgga aaaattttac atgtttttca
    29581 atccgaaatt ttcattaata cgggcaaaaa aaataaatgg caaatggaat gtaaaatttt
    29641 ttgccagtaa tatccctgct aatgagagaa tgaagcttga cagcaacgga cagccggtta
    29701 tggatggtaa aaattcggtt atggaacccg ttcctctgag tgaaatgtcc gaaaacgact
    29761 ggcgacgtta tcctgaaaca accgaatggg gaattggctg ggtgccactg ggaggttact
    29821 gtaaaatttc cggtatgatt gatgagtcga tggataaaac gcagatggcg caaccaccaa
    29881 aaccctggga ataccgttcg cgtactgtgt ggcaacgttt accaatcatt atcggaggag
    29941 tagtggtcaa ttttattctg gcgctggtca tttatgcagc tgtgcttttt acatggggca
    30001 aagaatacat tccgttacag aatgcaaaat atggacttag ttattcaaaa tcgatgactg
    30061 atatcggatt tcagaatggc gataaaatac tgaaagttga cgggcaggaa gtgagaacac
    30121 gcgctgatgc gatcgaaaaa attctgattg attttgcaaa aacggtcact gtagaacggg
    30181 gcaaccaaat cctggatata aaaatccccg aaggacttac caaaaaaata ctggcagccg
    30241 gagaagccaa tctgattgat atccgctatc cgtttgtggt ggccgaagtg atgaaaggat
    30301 caccggccga aaaggcaaaa ttaataccgg gcgacagtgt tattggcgtt aacggtaaag
    30361 aaatgaacat ttttcaggat atttccgctg aactgagtgc atccaaaaat tcgctcatca
    30421 cggttactta tgtcagaaat ggaataaccg aaaataccga aattcaaata aacgaagacg
    30481 gaaagctggg tgtgtacatg aacccgccca caaaatatat tcaatccgaa aaagaagaat
    30541 atagtttctt aaagtccatt cccgccggta taaaaatggg tgtggaaaca ttatccagtt
    30601 atatcaagca gttcaaactt gtatttacca aagaaggagc caaatccctc ggaggttttg
    30661 gttctatcgg ccagttattc cctcctgtat gggactggca ggcattctgg atgatgactg
    30721 catttctgtc ggttatactg gcatttatga acttcctgcc tattcccgga cttgacggag
    30781 gttacgtatt attcctcctt tatgaaatga ttacaggacg taaacccagt gataagtttc
    30841 tcgaatatgc acagtcggtg gggatgattt tattgctcat cctgttgatt tatgccaatg
    30901 gaaatgatat tttcaaagca tttttcaaat aaaaaataag tgtatgatta cagttttgtg
    30961 ttatccaaaa tgcggcacct gtcgcaaagc cgaaaaatgg ctgaaggaga ataatatcga
    31021 atacacatat cgtccgataa aggaacaaaa tccaaccagt gacgaactga aacaatggct
    31081 cgggaaaagt ggactgacta taaataagtt ctttaatacc agcggactgc tttacaagaa
    31141 aatgaacatg aaagagaagg tgaagcaatt gcccgaagat gaattgctcc gaattctggc
    31201 ctccgatgga ctgatggtaa aaagaccgat tttgctgaac ggtgaaaaag ttctggttgg
    31261 tttccgcgaa gaagagtgga aggaaatgtt cagtgtacga tagaaaaaaa ttataagaga
    31321 ggctgatatt taatgtcagc ctccttagtt ttatcaatca atgttcacat gagtaaaatc
    31381 cagaaaacga atgtagcccg attgctcgac aaagccggta tcagctatga actgattgct
    31441 tacgaggtcg atgaaaccga cctttcggcg gtgcatgttg cagcggaact gaacgagaat
    31501 ccaaaacaga tattcaaaac ccttgtgctg gaaggaaata aaacgaaata ttttgtctgc
    31561 attattcctg ccgacgaaga agtggatctt aagaaaacag cagccgtatc cggtaataag
    31621 aattgcgatc ttattttaca aaaagatttg cttccggtaa ccggatatat ccgtggagca
    31681 tgttcgccca tcggaatgaa aaaacaattt cccgctttca ttcacaattc ctgctctgaa
    31741 tttccctaca tatacgtcag tgcaggccag agaggtttgc aaatccggat tgcgcctcag
    31801 gatttaattc agatttcaca ggccagacct gctcagctaa tatagcagat ttattcttcc
    31861 cctggttatt aaaaaaactt atattttgca catttaatgc gtaaatgtgc aaaatcaata
    31921 tatctttgcg tcctgaattt ttaaactaat ttttttatat gaaaaaactg attatgtctc
    31981 tcatgctgct ttgcctgata gccgcagcat cgttcgcaca aacggataaa attgttggaa
    32041 gatggaaaac cattgatgat aaagatggca gtgaaaagtc tgttatcaat atttataaat
    32101 caaccaacgg aaagtattac ggcaaaattg agaaactttt taaggagccc gaaaaactgt
    32161 gtaccgaatg tgaaggtacg cataagaata aaccggtgct gggtatggtt atagtgaaca
    32221 atatgaccga aagtgaaggc actctaaccg gtggaaccat tcttgatccg aaagatggta
    32281 aagtgtatca atgcaatatc agctataatg ccaaaaaagc aaacctgaaa gttcgcggtt
    32341 cgctcgacag aaaaggtatt attggacgta cacaatattg ggtaaaagtc aaataaaatc
    32401 aggttcggaa aatattctcc aattctccgg ttcagcaaaa ttaaaaaatc agatgcagat
    32461 aagtactcgt ctgatttttt cgtttataat aagtttgttc taaaaaaatg acttaacttt
    32521 acagttcatt tttctttgtg gttgtctatg tcgaaaacaa tatctttcaa tcctctcact
    32581 ttgcccacgt atgtgatggc aaaaccggca ggttcgctgt gtaatctcaa ttgtagctat
    32641 tgttactatc tcgagaagga aaagatttat ccgaatcgta aaaaaacaga aatgagcaat
    32701 gagcttcttg aactttttac gaaaagttac atcgaagcac agcccgttaa agaagtgctt
    32761 tttacatggc atggcggcga aacgttattg cgagatattt cattttaccg caaggcgctc
    32821 gcatttcaac gaaaatacgg cagaggccgg attatcaata atacgctgca aaccaacggg
    32881 gtattgctca atgatgaatg gtgcacgttt tttaaagata ataatttcct cataggtatt
    32941 tccatcgacg gtccggaaca ttgccatgat gtgtaccgga aaaacaaggg agacagggga
    33001 acttttgctc aagtaatgcg tggtatcgga ttattgcaga aacacgaagt ggattttaat
    33061 accctatcgg ttatcaacga ttataatgta ctgttcccgc ttgacgtgta ccggtttctg
    33121 aaagaaatcg gcagccagta tatgcagttt tcgcccattg tggagcgggt ttcggactat
    33181 cggcccgatg gactgaaact tctgccaccc gaagaaaata ttgaagctga actggcttcc
    33241 tggactgtta atccggttga attcggacga ttttacatcg atatttttga tgaatgggtg
    33301 cggaatgacg taggaactta ttttgtacag cttttcgact ctacactggc caatatggta
    33361 ggcgaacaac ccggtgtctg catttttgcc gaaacctgcg gccatgctgg agcgctggaa
    33421 ttcaacggtg acctgtatgc ctgcgatcat tatgtatttc cggaattcaa actcggaaac
    33481 atcaggtctc agaccatttt tgaaatgatg tttcaggaaa gacaacttca gtttggcgca
    33541 gataaaaggg ataagttgcc tctgcagtgc cgcaaatgtg atgttctgaa attctgccat
    33601 ggagaatgtc cgaaaaatcg cattgcccgt acgccaagcg gagaatccgg actgaactac
    33661 ctctgcgaag gttacaaggc ttttttccgg catgcattac cctacatgga gttcatggcc
    33721 aatgaactgt acttcgagcg accgcctatc aatgtgatgc actttgcccg gggcagacat
    33781 taaattacga ataaacacaa gcaaagatta ttattcttca gcaactgtca tttttcatta
    33841 cagttacggc acatgtcaaa ctgtttccgg ttatgcagca tctccttccg gaattctgca
    33901 gctttatggc tgctgaagca ttctccgaac gatttttcat tcagattacc aaaaacaaat
    33961 gagcggtcct tgtcgtagca acagggaaga acgtcgcccc tcgaggtgac cacactgcca
    34021 ctccacaagc gtttgcatcg gtttggttgt gagtttttca ggtgatattt tccatctgat
    34081 cttaattcgt atcgggcgta tcgtttgtta tcaggtaata gtgaattacc gttcacaaag
    34141 tcatacagtt gagcagactt aaaggtcagt ttgtctgctt tcagcttttt tgccagaagt
    34201 ttcatggcct ccatttcgtg ctcattggtc ttcagtacca gaaactgtat ttcgattaac
    34261 ggagtgcgcg atttcagctc atttctccat ttaatcacat tctcaaccgc ttgcaatgcc
    34321 tttttgagtt gtccgcctgc gcggtaggct tcgtaggttt cctgtgttgc tccgtcaatg
    34381 gaaacgataa gcctgtcgag tccagactct actacttttc tggctgtttg gtcagtcagc
    34441 agttgagcgt tcgttgaagt acttgtcagc aaatttttgt tatgggcata gcgaaccaga
    34501 tttgtaaaat tgcggtttat aaaaggttct ccctgaaaat aaaatataac atgcgaaagg
    34561 gtttgctcaa gttcatcaat cagatggtgc accatttttt cattgatatt taccgcttcg
    34621 atacgttcag tacggattcc aaccggacat tccggacaat gaagattgca tccgttgacc
    34681 ggttcgattg aaataaacca gggtgaatat ttccatcggg tttgaattcc aaaaaatgaa
    34741 agctgatatg atacagccag ttttatggca ttgagtattt tacggggagt gaatcctgtt
    34801 tttaacaccc tgagtatata atgtaaatga ttaaaagtca tcggacaaat attaatttat
    34861 agagcccaaa agcaaatttt caaacacaaa atgtaaaatt ttattgtagc aaatcaaaaa
    34921 agccaaaatc cgatagcaaa gtaagataaa ataaaattaa aaacgtatct ttgtagtaat
    34981 attttgaaaa tcacaaacta ttaaatatca atcaattaga attatgaaca aaaaaatttt
    35041 aatagctgta agtttggtta tggctattgt ttttgtatca tgtaacaaaa acatgaaagc
    35101 attggatgca agctatttca aaacaacacc aaatcctctc gaagaaaaag ctggtaaagt
    35161 ggatgccact gttaccggga ctttcccggt taagtatttc aacaaaaaag ctgtagttac
    35221 tgttactcct gttatgaaat tcaacggaga agaagttaaa ggacaatcat ctgttttcca
    35281 gggagaaaaa atactggata ataatcaaaa aattgcctat aaagcaggtg gtacttacac
    35341 aatgaagcct tctttctttt atatacccga gatggctaaa tcagaacttt atcttaattt
    35401 tgatgtagta cagaagaaaa agaactatac attgcctgct gtaaaaatag ctgatggagt
    35461 gcttgctact tcagaacttg tcgcagtgaa agatattaaa cctgctctga tagcagataa
    35521 atttcagcgt attacacagg aaaaccagaa tgccgacatt cttttcctta tccagcaggc
    35581 gcaattgcgc aattctgaac tcaaagctga agatgtaaaa gcattcacag caaaggttaa
    35641 agaagcccgc gatgcacaaa acaaagaagt aacttcgctt gaagttctcg gttatgcttc
    35701 acccgatggt gctgttgacc tgaatactaa tctggccgaa aaacgtcaga aagtaacaaa
    35761 agattatgta aacaaggaac tgaaaaaact gaaatcaaac gtaactgttg acagtaaatt
    35821 tactgcagaa gactgggaag gatttcagaa tttgatggaa agttcaaaca ttcaggacaa
    35881 ggatttgatc ctgagagttt tgtctatgta tcaggatccc gaacaacgcg aacgtgaaat
    35941 caaaaacctc tctactacat tcaaaacaat tgctaccgac attctcccgc agttacgtcg
    36001 ttcacgcatg aaactcacag tagatgttac cggtaaatcg gacgaacaaa ttgctcagct
    36061 tgctgcaaca tctcctgcag atctgactgt tgaagaactc ctttatgctg caacccttac
    36121 caaaacagaa acggagaagg cagcaatcta tcagaaagtg atcagcttgt atccgaccga
    36181 tgcacgtggc tacaataatc tgggactggt gaattacgaa atgggaaaaa tcgcagaagc
    36241 acaaaaactt tatgaaaaag cacttgcaat tgaacctcaa tctgctgatg caaattacaa
    36301 tgtaggatta atttcgctct cgaaaggtgc aattaccgat gcgctggcat attttggtaa
    36361 agctgccggt acaacaggag atctgaactc agccctcgga actacctata tcgcacaggg
    36421 agattatacg aaagctaaaa atgtgatgac tgaagcaaat acaaacaata cagcactggc
    36481 gcaaatactg tgttcagatt acaacgctgc acgccgcaca ctggctgcag tagcagctcc
    36541 caacgctatg acagcatatc ttggcgctat tatcggggca cgtaccaacg accgcgatgc
    36601 tgtttacaat aacctgaaaa cagcagtaca gaaagatatc tctctggcca agaaggctct
    36661 gactgacctg gaattctcaa aatatttcac agatgccagt ttcctctcta tcctgaaata
    36721 attattcatt aaattctata tactaaatac cccgatgcct tgtaaggtat cggggctttt
    36781 gtttgttggt aggtatcatg agtgaatgat tcaggattaa aacataaaaa tgtagtttca
    36841 cttggccgat aatattaaaa atcattaccg aaaatatttt cgaactttat ggaattaaaa
    36901 tttattcttg tacctttgtc aattaatgga tattgccata atacgctttc gctgaaagcg
    36961 ataagttttt tgaaaatata atttttgatt tctgtgttta gcgatataat aggacaacat
    37021 acaattaagg aacgttttat ccgcactgta cgtgaacagc gcatacctca tgcccagctg
    37081 ttatacggac ctgaaggtgt gggaaaactg gcattagcca ttgcttatgc acagtatatt
    37141 tcatgcacca accgcactga aacggatgct tgcggagtgt gtccttcgtg tgtaaaatac
    37201 aaacaattgc aacatcccga tctgcatttc gttttcccga tcataaaacc tgcaggaaaa
    37261 tcatcgataa tttgtgatga ttttattggt gaatacaggc aaatgctgat cagtgaccgg
    37321 tatttcagtg tcaacgactg gtacgagaaa attggcgaag gagccaaaca gggaatgatt
    37381 tactccggcg aaagcgtaga aattatccga aaactcagcc tgaaaaccta tgagtccgaa
    37441 tataaggtga tgattatttg gctaccggaa cttatgaaca ttgattgtgc caataagctt
    37501 ctgaaaatac ttgaagaacc accagcattt actgtttttc tgctcgtatc caacaatccg
    37561 gatgccaata tcacaacgat tttatcgcga acccagcatg tgaaagttcc cagactggaa
    37621 gaacacgaaa tagttgatgc gctgatatca agatatcctg aatcgaacaa agcagacata
    37681 gaaaacactg cacgcgtggc agagggaagt tttttacaag cttgccgtat attgaacgag
    37741 aaaggtgaaa gttcggttaa tttcaaccga tttgtcgaga tcatgcgatt ggcctggtta
    37801 gtgggtaata aaaaggacca tcaatcgctg aaaacactca aaaaatggtc ggacaacatg
    37861 gcgagtgccg atgtgggtcg cgaacgtcag aagaaatttc tgaattacgc gcagaacatg
    37921 gttcgcgaaa actatattta taatcttcgg caacccacgc ttaattacat gacggattac
    37981 gaaagtaaat tctcagccaa tttctcaccg tacatcaacg agcgaaatgt tgaagacctg
    38041 atgaaggaat ttgccttgtc ggaacggcag attgagcaaa atacaaatgc taaaatggtt
    38101 ttctttgatt tggttttgaa aataattatg ttgctgaaga ggtaacaata ataaaaaaat
    38161 aagttttgaa ataaatacga aacactctga cgatacaagg tgttaatgaa cttgcagata
    38221 cgcaaatata cgatgaaaat ggtagggatt aaaaaagctt acgaatgggt gtatccgaat
    38281 gattgaagaa tctgtgcaag taaaataagt cggagtaaat atataattaa atctgaaata
    38341 agatggaata cagactggat aatggcggat gccggatgaa taaaaaagga tgctgcaaca
    38401 cgacgagaca aaaactgagt gtttacgact ggctgtgcga tcttcccgaa accataaagg
    38461 atactgattt tgtggaagtt cagtttaaaa atacgcggaa aagctattat ctaaacagcc
    38521 cgaaaataga actcgaaaaa ggtgatatgg tcgctgtgga ggcttcgccc ggacacgata
    38581 tcggtgaagt aacgctggtc ggcaaactgg tagtattgca aatgcagaaa aacaacatcg
    38641 atttcgaaaa gtttgaagta aagcgaatat accgcaaagt gcgtgaagcg gatatggaaa
    38701 agtacaacga agcgaaagcc cgtgagcagg aaaccatgat aaaatcacgt cagattgcag
    38761 aaagcctcca tctgaacatg aaaatagggg atgtggagtt tcagggtgat ggaaacaagg
    38821 ctatatttta ttatattgcc gatgagcggg ttgacttccg ccaactgatc aaagtctttg
    38881 ccgaaacatt ccgtgtgcga atagaaatga aacaaatcgg agcccgccag gaagccggcc
    38941 gcattggagg tatcggtccg tgcggacgcg aactttgctg ctccaagtgg atgaccaatt
    39001 tcagtacggt ctcaaccagt gcagcacggt atcaggattt gtcgctcaac ccacagaaac
    39061 ttgcaggaca atgtgccaaa ctcaaatgtt gcatgaattt cgagctcgac gcgtatatgg
    39121 atgctaataa aagtctgcct tcgcgcgaag tacaactcga aacgagggat gctctttatt
    39181 atcatttcaa aaccgatatc ttcaaaggat taatcacata ctccactgct cccaatttcg
    39241 cagccaatct ggtgactatc actgctgatc gtgccaagga aattatttca atgaacaaaa
    39301 aaggtcagaa acccgctcag cttgaagtgc cgcagaatga agagaagaaa caggcagaat
    39361 atgaaaatgt ggtaggtcag gacagtctga cgcgtttcga tgtgaagcac aaaaatactt
    39421 ccggtcaaaa tcgtggaaac cggccatcaa agttaaaaaa gagcaataat ccaaacacaa
    39481 aacaacttaa taaacgaaat gataaagaaa ataaataatt tctttttgtt gattgcagta
    39541 gcagttgcag tttcttcgtg caatcagaaa gatgtttatt tcagttacca gacggtgtca
    39601 gcttccggat ggaacaagga cagcctcctt acatttagtg ttggtatcag tgataagact
    39661 atgccctata atatgtacat aaatgttcga catcacggaa attatcctta tcagaattta
    39721 tggctctttc tggagaataa agacgtgaag ggaaaggtca caaaagatac tatcgagtgt
    39781 tatctggccg atgagtttgg taagtggtta ggctcgggtg caggagcgct gaaagaaatg
    39841 cctgtattat atcgtcagca ggtatttttt ccggatagcg gcacatacca gataaaagta
    39901 ggacagggaa tgcgtgacag cattctggtg gggattaatg atattggaat acgtgtagag
    39961 aaagcaaact gataaatggg aaaaaataaa ctggcaaaat ttgccgaaat ggacacgttt
    40021 ccacatgtgt ttcaggtgcc gtcagcttca ttgctcgatg gtaatggatt tgaactaaaa
    40081 ggaaaatgga atgaggcttt ctttaaaaat tcaaatccga tagtactgga actgggttgc
    40141 ggaaaaggag aatataccgt ggaacttgca cgcaggtttc cagacaaaaa ttatatcgga
    40201 gtagatatca aaggggctcg catgtggacc ggagcgaagg attcgtttgt caatgagata
    40261 aaaaatgtag cattcatccg tacaaacatt gaaatgattt ttcacttttt tgccaccggt
    40321 gaagtaagcg aaatctggct tacttttcct gatccgcaga tgaaaaaaac aaccaaacga
    40381 ctcgctgcca ccaattttat aagctcatat ctcaaattcc tgaaaccgga tggggttgtt
    40441 cacctcaaaa ccgacagtaa ttttatgttt acctacacct gtgccatggt cgagttgaat
    40501 aatttcccgg ttattggcca tacggacgat atttatgccg gtgaacatga aaaaaatcct
    40561 gtacttggca ttaaaaccta ttatgagcaa cagtggctag aaagaggact gactatcaaa
    40621 tacatagcgt tccatcccct agccggaaaa acattgcttg aaccggatat tgacatcgaa
    40681 caagacattt atcgcagttt taaacgctat aaacgtcaac aatagatagc ttaaattgtt
    40741 ataggcgtta aaatcaaaat cattttaaat aataaaaacc caatgacact atatccaaat
    40801 ttgattcttg aggcattgaa acatgtccgc tatccgggta ccggaaatga tatcgtatct
    40861 tccgaaatgg ttcaggataa tatccgaatt gaagataaca aggtaagttt ctctctcctt
    40921 ttcgaaaagc ccaacgatcc gtttgcaaaa tcggtcgttc gtgcagccga acaggcaatc
    40981 ctgacctata taggagaaga cgttgaaata aaaggaaaca tcagtatagt tacaaaggaa
    41041 ctacccaaac ctaaacccgc atctattttg ccagacgtta agaatattat tgcggtattt
    41101 tcaggaaagg gaggcgttgg caagtcaacc gtgtcgtcca acctggcaat agcgctggct
    41161 tcgctgggtt acaaagttgg tctgcttgat gccgatatac atggtccgtc catccctaaa
    41221 atgtttggag tggaaaatga gcgtccgttt gtagaagaaa tcgatggaaa acaactgatg
    41281 aaacccattg aaaagttcgg gataaaactc ctttcaatcg gttttttcgt cgatccggag
    41341 aacgcgcttg tatggcgcgg aagcatggcc agcaatgctc tgaaacaact tattactgat
    41401 accatgtggg gtgaactcga ttttttcatc atggacctcc ctcccggaac cggcgatata
    41461 catctcaccc ttgttcaaac catgggaatt acaggtgcaa tagcggttac aacacctcag
    41521 gacgtggcac ttgccgatgc ccgaaaagga attaatatgt ttacgggtga gaaagttaat
    41581 gtacccgtac ttggtattat cgaaaatatg gcatggttta cacccgccga actgcctgaa
    41641 aataaatact atatttttgg caaagacgga ggtaagaaac tcgcggaaga acttggtgtt
    41701 cccttgctgg gtcaaattcc gctggttcaa agcatccgcg aaggtggtga tgctggtatg
    41761 cccattgcaa tcaatgacaa cagcctaacc tcgatggctt tccaccatct ggctgaacag
    41821 gtagtcgaaa gggtggaata caggaataaa actttgattg ctacacaaag ggtagaaatc
    41881 gtaaagtaat ataactcaat cagtttttaa ttaatgttat atgaaattat ttgttgattt
    41941 tattaaaaaa atcaacaata ttaacatgta aaataaaaat aaatgttact tttgtaaaat
    42001 tttcggaaac aatacatgtg ttttattatt tacaattaaa actttatgaa aaagactctt
    42061 atctatttgc tgcttctaag tatttcat
//
LOCUS       DAOPYB010000008        41795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_802_length_41795_cov_7.139698, whole genome shotgun sequence.
ACCESSION   DAOPYB010000008 DAOPYB010000000
VERSION     DAOPYB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 41795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41795
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_802_length_41795_cov_7.139698"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..917
                     /locus_tag="PKH58_01290"
     CDS             57..917
                     /locus_tag="PKH58_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX87689.1"
                     /translation="MPIRTIMEINNIYAMNANDYLIKFETEMRLINYCNRTIANYISH
                     TRIFLLHFDKDPHEINEDEIKEYLKKSSSQAQLKQRIGSIKLFYEKVIHDPLKFKYIQ
                     YPRREQTLPDVLSQDEMRRLFAACSNLKHLTILQLFYSTGMRESEVINLRVTDIDSNR
                     MVIHIRQAKGKKDRLVPLSERTLITLRQYYQSYKPKEYLFNGQFSLQYSAKSIQQFIH
                     DYAAKAGITRRVYPHLIRHCFATHLYEGGADINLIQALLGHKSVKTTMIYTHLSNRLI
                     SQVRTPDMVL"
     gene            1502..1996
                     /locus_tag="PKH58_01295"
     CDS             1502..1996
                     /locus_tag="PKH58_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87690.1"
                     /translation="MAITQYSTIQLKIRKPYFENYLRWVFNSQEGDIKVNRDEILGKF
                     LFSMARTSDIPIKVRNQTGIVKLIMPSHICDDRSKYRSIYYSVDDENHINDYIEACAF
                     MDLRMMVQTAKLDMKMDRKTVFEIYSEMIFGENKYEALKKYEYRRRKKALDYLRVTAK
                     ALCM"
     gene            2070..2447
                     /locus_tag="PKH58_01300"
     CDS             2070..2447
                     /locus_tag="PKH58_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87691.1"
                     /translation="MIITSLSYALSSSGSWIELPVQGHSGSINEKNVTTEEGLVSETE
                     VNAYIAGLSHANDMMLGYLTRRKAMYRAADADGRYHYIGTVDFGASLEIEQRLDGSPG
                     TKYGYNIRITCRSVAGTQKQSFR"
     gene            2487..3737
                     /locus_tag="PKH58_01305"
     CDS             2487..3737
                     /locus_tag="PKH58_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNX87692.1"
                     /translation="MLQPHLIYSILNEPWAISEQVFNGYVHLLESVLNPNISFEKGEP
                     VDPEMHDINFRIKGSAAVSDSGQSDVKHIQVITISGVLTKEDQECGPAGMARIGNWIK
                     AADRDPGIDAILLKIDSPGGSVAGTETLSSIIKNCNKPVLGFADDMACSAAYWIISSC
                     AKVIANNSTAMVGSIGTVMEFVDEQPKLEKEGYVFHVVTAPQSVNKRRNIDKIRSGDY
                     EEYKETVLRPITQVFIDAVKANRGNIDEKYFTADVFFAKDVIGDLVDSIGTFEDALQE
                     AANLAAENVSASHKSTHISMQKPELKRLAKAAGSETFETVDGSITLTEEQATAAEGVL
                     EAHEAKIADLQQKVDQSSQLQSENNQLKSDLKAKNDRIAELEKGAGADSAHVEKDTDA
                     SDENSGNESFYERFNRLSANNKQQ"
     gene            3743..4705
                     /locus_tag="PKH58_01310"
     CDS             3743..4705
                     /locus_tag="PKH58_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87693.1"
                     /translation="MSVTIHQTLIDAGVKYKKDLLTMPVAVLSEILQYMTLRTGLQGK
                     EIGGVLDTDAQLRPYRTAKDASDNTTITPYEWETFLGDVVKEFDPNAILGTLYTEATN
                     KKPTEREIARLVALEMAKKVGEALYDNMFTAVRNASGSTTADLFNGFSTQVAAAIVAG
                     TLASGNGNYQDLSSEAIDYTNVGDVLKTAWRSCDKLLKKRAVNLYLPTSILEIYEDWF
                     QAEYGHAPWNTDFSQMYLIGTQKKVRLVPLDNMESQDYMFFTIRENLKVGVDQESDKE
                     DVKIRECDNPKMVQFFMMSYFGVGFDTLDKRFIKVIKFSTEAAS"
     gene            4718..5305
                     /locus_tag="PKH58_01315"
     CDS             4718..5305
                     /locus_tag="PKH58_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87694.1"
                     /translation="MAELFEQLEWLDGQINPSGIKNIVYFAPKSWIKTIPRVTTPVAT
                     STENVVVSGDFIMESGKTFLRLYSTQGKGKVSWEPSGEKDHKIFLNKGIFSFPDISVA
                     ARSMAKQLINSNVVIIVPLPHETEKRYIMLGDADYDVTVSIKGDSGDKPGSEKGITFE
                     VEAPCTTPLPGYAGELVLPDGTLDCETGVFTETAS"
     gene            5386..6147
                     /locus_tag="PKH58_01320"
     CDS             5386..6147
                     /locus_tag="PKH58_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87695.1"
                     /translation="MTPHEEITAYLEKQEKTFDEGFSIFRKYSRNKSLEHFLERKRDM
                     AKLAYELRKLLGSKIKDSTVSTLNLIKSQPAPAVNPVVPPGPDGATFRYLRFQKINPE
                     ELPADVRRIYDQIAEAYKMQRTCHEKMKLAKTDKTRADWRKKVVEFDDVISSGWNGID
                     AFVAGSDTIPDTANAGNITDVSKEINACRSYISRAINDVPKLSSDKREKRIKETQQRL
                     SRLAALKTPITSETRDRLIEAGIIDLDYQVLLESA"
     gene            6188..7651
                     /locus_tag="PKH58_01325"
     CDS             6188..7651
                     /locus_tag="PKH58_01325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX87696.1"
                     /translation="MATMPSRVVALRDTINTILPQCDELNLYLNNFINVPEFLGHQKI
                     KLYRSQDEWGDIGDVGKFYRVNLQQGYIFTVDDKIIYPADYVQQMVTTIERAHRKAVV
                     SNHGRLFHKDKPTKSYYFDTAKNFVYSLAYPLTFVHEIGTGVLAWHSDTCRPDMSWFP
                     HTNMTDIYFSIEVQKRNIPIIIHPHENGWLKLGTKHDENYSIHANCNRKDKFQTSVVN
                     AFSWKINTCPVDQPAMGKSGKDVTFAGLDCRYRVPELDLAIVIPVYNQLEFTRNIFKC
                     LPLTRSKFTVILIDDASTDDTPIFIDEMMKQSAMSIIYRRHETNKGVNASWNEGIRIS
                     RAIGANHIAVLNNDLELIKNWDMPMIEMLSDATVGIVCPYSTHGKSLPVNFGTYPGGK
                     NHLALDILGCCFMFRPDLIDTIGYIPDSLVTYYGDNWLQEITRMAGMKVVYAPGSRVH
                     HYFQQTTSKISHKQILADDAEAFNKLCASGLLNNITL"
     gene            7654..8199
                     /locus_tag="PKH58_01330"
     CDS             7654..8199
                     /locus_tag="PKH58_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX87697.1"
                     /translation="MPIGSVVLLPHVISEAQKIKPSCILDVGIGYGIYGPAFRQWLDM
                     GVKPYKTIVDGVEGFRQYKSACWDEYNDVVISDIRDFTSQFTYDMIIFSDVIEHLTKE
                     DGLCEIEKLKRTLKNNGVLLIGTPALWIPQTDVHNNEFERHKSLWTVEDFPGFEVILD
                     GSPVLGNRMILVKYTKHEPGT"
     gene            8183..8785
                     /locus_tag="PKH58_01335"
     CDS             8183..8785
                     /locus_tag="PKH58_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87698.1"
                     /translation="MNRGPKKNLDKFQAIMFDDLASLDHLNQDEKDQLRRYRFAFTRL
                     LDNPSMSDVTLRDELMSQFGIKQTQAYADISNLKIILPNIRNAGKEWIRYVVNEELKD
                     AIAQCKSYGGDKLKELILAIDKLAKYNKLDQNEGEEIDWDQIYPQPIEPTGDISVLGE
                     IPMENKRETIEKLITKFRNEIEIEDIECEDVSNGEQESIL"
     gene            8760..10376
                     /locus_tag="PKH58_01340"
     CDS             8760..10376
                     /locus_tag="PKH58_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87699.1"
                     /translation="MENKKVYYNDKQLEFRYIAAHTSVIVGGRRFGKGHGINAPWLLR
                     NVQYMPRSGGGIVGSTFQQLLTRTIPGTLKSLEDMGFRRNIHYVIGRRPPESLGFKDP
                     VIKPVSYDHVISWYNGSVNYLISQDVPGSSNSLTLQYILADEAKFLDFEKMKDETFPA
                     NGGYKGPWSKCPWLNSILITSDMPTSKKGSWFLNYKDKMDPEVIDSIKWLLKEINRVK
                     NLNTRKYNEALKFYRLRLAQLRSIAVYYREWSTIENVELLGQKYIAQMKRDLPPLVFM
                     TSIMCIRPGKLKGGFYPNLRESVHYYTAYDNSYLQNLDYDLDKAQDMDCSQDADVDLS
                     RPICVAFDYNANINWLVCGQQRGIKALVLKSFYVKYQRKLRELVDDFCEYYRHHHTRE
                     VIYYYDNTALGSNYAVSDEDFASVICNQFEKNKWTVKRVHLGNPLKHHEKYNIIDQAF
                     KGQKYLMPQYNKPNNEALLLGMEHCGVKIGPLGFQKDKSGEKLAETEEDLLEYRTDGT
                     DAQDTLLLGMFFNPDNSTGVMMPMSGSVIL"
     gene            complement(10590..10850)
                     /locus_tag="PKH58_01345"
     CDS             complement(10590..10850)
                     /locus_tag="PKH58_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87700.1"
                     /translation="MAEMKHPLTTQIEASLQTVCTESSVLYFIMSPVIGGAPLANTAT
                     MASISMTNKIFFIFKRLVVKGLIVREIKNNPVKLVSKGHFRV"
     gene            11143..11493
                     /locus_tag="PKH58_01350"
     CDS             11143..11493
                     /locus_tag="PKH58_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87701.1"
                     /translation="MIDLTKAVNNNKFLRELYGIFASVTGYAPLKAQSVNSLGNTNSG
                     VCGGCVSVTTSETAFDYPSFIECTGNDGTIDYTDEYGNVVAGYPMTQGKTTNFRVNKV
                     TAATATGLYRCYAK"
     gene            11524..14337
                     /locus_tag="PKH58_01355"
     CDS             11524..14337
                     /locus_tag="PKH58_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87702.1"
                     /translation="MAVDCRAQLGSNVNLNGLKGLMSFWSDSFNFTTNKLTDKSGNAD
                     NAPSLVDSNCLVFNGTSTVVTISGTYVQNIAKVLIYAQSGSDAWTIFTELTAPNGLYS
                     ISGNILYIGKNGAAFFAGKIARIKCYSSADRLLADIPLSMGYGTKVYGLVPDNNFLDS
                     TVLSASLEGTFIDTNADGVPDPLIPSTNWTKVTQTIVNDAINGWSGRHGRIDFTTKGD
                     GTYYYLQGGVTPGELLTAGYFKIRFRTNISSANKIWVRYGNYMTTVSQYLKYPVSGAI
                     TEGEMYFDASNCATLTIWFKHTEENTYFEYDFIQLRKYAGYGIITDGVWSATSPNAHC
                     NLEMGVDVYVNETNKIYVPKINSEKSHYPGTLTFLKSYAPATNSLIDVETKLQFPDVE
                     ALKTTDSKNYLYNSGGTALKLGYSDLVGAKGDYFFSNVYTPKEGINDIVLLKENVTTR
                     NLRTINTNLKKSDKRIYVKYISPYLYINTKLSFGKHLFTKVPLPSLQTNNIGEMYLED
                     TEYKLAINSTSSLLKNCTDDSAPVNVNGSYVGGNHAYAGMMNVTLVGHDKDASDIGSV
                     WSTGGFNYKLVKIVGNVLSFIQSGTATHYGYKYKTTFSSGTLIHVSGATHTGDISAAT
                     TVPNQYISPGKVRTLTNILADGLVISDGFDGYCNELEIQIIENIYNYLVIEDYYPNDL
                     ANCPFIIRRNLSYIYTSDGSMRTKETVTALLDDVYFSGQMGIDQAAPLNSAMSGVTKQ
                     YFYLPGSAPFTIGSNAYDLRQMSDWFSFTPDTILFTPEKRETGKHINRMVEIISSDAE
                     KMYGFSHGYFLRDNSVNVADIDWYLVNTKKSYPEYATNIALLKDTSISGESFRKYFDA
                     LRTDSATCAYIVKADYGYNLYIDVHTNLSDAWISVPSEVVGMNIAIDDSNENITIHST
                     QVISDGIKVTVINNYGYAVLRLT"
     gene            14360..14686
                     /locus_tag="PKH58_01360"
     CDS             14360..14686
                     /locus_tag="PKH58_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87703.1"
                     /translation="MNIITISIAALIYLFYQAADAWGDVAIELGHQQPWHTWGAVQAG
                     MFIAVLAFALCGWSWCTVIVSASLVVLRFPIFNLMHNAAKGQRWCYLSDNGIDGLIKK
                     LLSWLT"
     gene            14674..15228
                     /locus_tag="PKH58_01365"
     CDS             14674..15228
                     /locus_tag="PKH58_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87704.1"
                     /translation="MADISTLQVGDIILVRDGKSFIASGITWWMKIYKKKKGITLLAT
                     YHHAGTVITDESVLRIAEAVGKGYQINSIEKAYSLQEWGKRIDVISPDTPYTEKEKKW
                     LSQKAKEYNQEITRYDFLNFFFQIWLIKTGKWIGPRGKKAENRLYCSEAVATIANYVR
                     PCTFVNPAATNPMDIAINHNYHFI"
     gene            15291..15512
                     /locus_tag="PKH58_01370"
     CDS             15291..15512
                     /locus_tag="PKH58_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87705.1"
                     /translation="MILANNLHKLVEKGEFSIAFIAEEGNIVTCDRAICTSWHSSGRT
                     MNIKFLVSKQIRTIRRCTIIAFNDEELVL"
     gene            15509..16855
                     /locus_tag="PKH58_01375"
     CDS             15509..16855
                     /locus_tag="PKH58_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87706.1"
                     /translation="MTTDTFVIDNITIMPGLAAIITESDSQDLIEDPTNTISIGENDK
                     IAPWGRDNDLPDKVFAKIMKSDVAASNLMFNIQACYGQGIKPMMRRIENNTVAYQECN
                     DEKVMQFFDDNDINGYFHEQCTDFITFFNAFPEIVLTADGRSVYSLRHKEAMFSRLGV
                     VNNKGEIVKHYYSSKWNSESGSANKENTVISEVLSHYNPYADLTARITKGNRTRRFIM
                     PVRFPVPGHIYYPKPYFWSMFRSGSYDFSSLIWKYKKTLLKNGLAVRYIIYIDEKYWQ
                     IIFDEEKIDRNDPKKVKDRKEEEFAKFRNFLTDEHNQGKGLMALKKTIQTGTGAIVEK
                     YIEIEEVKITSKGGEFIEDSSEVNNVMSYAMGVHPNMIGANPGKTGQSMSGTDKRELY
                     MIKTAMMKPFRDRLLQPLYLVKRYNKWPNELEFVVTDYQFPTLDQNKTGKQEVAQS"
     gene            16852..17688
                     /locus_tag="PKH58_01380"
     CDS             16852..17688
                     /locus_tag="PKH58_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87707.1"
                     /translation="MIITTIAQLKDRIATVKGADIKRYEPYLATADLFLQSELIGSTL
                     YGKLNATGNETLLTRCQDVVALKAYHDAIPFLDLIETETGFGVVSSGGSNLVPASRER
                     VDKLIAQTEIRLSGAIELLLQHLEETTAYHTDWATASAYSIIHDSYIFTLTEFRRYAR
                     YDASRLEFVKDAPKIQRAIRYIIEPVISRELSLAIITQLKAGNLNDANKVIIEDLRFA
                     LALYVTGEPVAASASLAKVKDVLYTNPDSYPEFKASNIYTAFLAQEERDTHLDPFMVC
                     GM"
     gene            17676..18452
                     /locus_tag="PKH58_01385"
     CDS             17676..18452
                     /locus_tag="PKH58_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87708.1"
                     /translation="MRNVTTINLQAPKAWNELSGSQLNFIARQLIRKAEAAEIMVNCF
                     LQFTGLSIKKKYPYQDNNGVWYRFRKRGVGSFDLDADRFTWLAKKLEWITDDISLFHN
                     PKRILWFTGCNYKMYGVSFERWFLLDQYYGAYCHSRDQESLNKMIAVCYRRKGEAWDD
                     GKNMDKRAWLFRFVPLYRKYVVFLWYTSLKKYLSFKYPHVFSSDSGGVTVKPSANELI
                     MGITSALNDGNITLNPQIRKTDVHEVLFELNRKIESSLKI"
     gene            18457..18891
                     /locus_tag="PKH58_01390"
     CDS             18457..18891
                     /locus_tag="PKH58_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87709.1"
                     /translation="MFALYEYLDSVKAALSIDKIYRISGILQLEEFLNGLGDAQDTVV
                     FARDSGDGYLNINDRRLDTAYHMFYVFQRAEINDNDSRLDAKRNSMAKGITIVEKIKA
                     ESKDFGDPAYGFNAARIDYSEIGPIARNYYGYSFAFIMENHF"
     gene            18891..19313
                     /locus_tag="PKH58_01395"
     CDS             18891..19313
                     /locus_tag="PKH58_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87710.1"
                     /translation="MAENININLTVEAWADIVIMEWIKKFEALGIRELHGIHSFESSI
                     ITASDGDPAKVRFAFEWYLKMVDYGVGKYVNLETRDTMIAAGLTTRRPKPWFSDTFYK
                     QLSVLHHLYEEKYAKKTELFIVRNLKDNADLGFNEVAL"
     gene            19310..21931
                     /locus_tag="PKH58_01400"
     CDS             19310..21931
                     /locus_tag="PKH58_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87711.1"
                     /translation="MSKAIIDVTLNNEEAKARLKEIAAELTKVKNLREQALEKGHASA
                     FDLANKEMKKLQAESKKLSKEVFDVNNVLKNISSASIKDLNTAIRTLSRDLDKMKRTD
                     PGYGEKQKALENLREEYNRATGKAKEHTGVLGRMGISFGSAMSIVGQFAAGLGIATGA
                     VAAIKGVIASTDTVSDRFNKTVGGLTSGLSYLGRAIATMDFSNFLSNMRSAIEEGQRY
                     VDTVDMIGDKTRALTIIESQNRAEIAKNKITARDVTLPYDERLKAANKVLDLEQKNAD
                     YRKEIADDVLQNELDSAKDITKLSGERIEGLLKEKLINKDNVALANQYLDALNEQKRV
                     EKQSASKVVGAPYYVQAGAGTALSEKSAKILRETPEDIKKYAQELKQFGKIAEDTSKD
                     SETSLNKLTKAFEGVGEAQAYYDETTSRTQKNRSTIIKEMLKDEASREKTIKNQDTAY
                     ENLNKQIQELEKTLADQVFRNDPKSAQTLKLLELAKAKAEEVKEILDSYNAAADIKIS
                     EKPDQYFTAGFDDEINAIIDKNDLETKLLDKKLDDQQKIYEDALKDKEKADKDYQAKK
                     EDMEKLAIDTSISLLNTAADLIYEKDMQAIEDKYSEEEARLEKMLNNNSISQQSYENK
                     VKLLREKRAKEEEKIEKQTSAIKKTIAAIGITADAAESVFKIKAMAAELTAKAAVLAI
                     TNPAAAALYPPLIATVLAQIPLILGSAALQITAAMASGGKKAGGFAATDKSDDTPMGY
                     YHANEFIGNADSVRNPSVRKIYDVIDYAQRNGTISNINLPAIISASFPGRESGGYTGL
                     DDDLNTLSDAKTKAWMAPGVYYAVIGDVLKEVALVVKDLKTRLDSPIEASVALRGSKG
                     LYEAMDEDNQLKNNASL"
     gene            21928..23727
                     /locus_tag="PKH58_01405"
     CDS             21928..23727
                     /locus_tag="PKH58_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87712.1"
                     /translation="MIEIIVNNTTRLQLPSKVKINIVQKNPLFLEDRIPSPYSLSFEV
                     PVTAGNLAAMGQPARITSSSIKARQPGKIMHNGMVIGAGEILLLEVNKVLKLQFKGSP
                     LNGNFNKNLNQITNDEYDYGTMTYHGEDINYSDTSFDAYVQNLRSEANNASHFVLAPV
                     KLADTEWNGTSSYLGAKNGLINYLNFWNVATASWFTGDVHYMHMPVVPFPYLKDTIAK
                     AFGSNLESNPFASGDLAKLVTISANHPHLNLNSLYDWYLTGPNLETKQEVFWPLLDSY
                     VSSNYLIPLKFNIMNFMQAYAFNEFLKEILKMFSMSAFPGIQYRIEKNNDIFDRSVVV
                     NWSDKLAGKPIIKTRDAKDYNFKFRNAGEKASDPVRTKPNIDAIYDSALEQGSEEVEY
                     SDAVSGAIIGITKELFGDASEPRLTQDTKQSALSATIEDTGLDKYEVNCEVEPLPMSI
                     EQYWSDNENPGNVISHKHWHVPVIEKPGLNDAPNIMIWGGMANTFEGDGTYPLLMNHH
                     TDHFGTKRGSLSLLPDGPDGLISTFHSGMKSWIECEKKSVKGSFRLSPLEIRNIDMRD
                     KIYLHGRLFYIEKIEYSLTHNDVSLVEADLIEC"
     gene            23791..24078
                     /locus_tag="PKH58_01410"
     CDS             23791..24078
                     /locus_tag="PKH58_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87713.1"
                     /translation="MTPFETWLYRGIIAVILLIVWYFIQRFVNNVQDKLDDIQDGINK
                     LNNQSGVHEERLKAMNMAIESQGERLNDHSQRLRMVETNQAKCRNCNNATK"
     gene            24093..24500
                     /locus_tag="PKH58_01415"
     CDS             24093..24500
                     /locus_tag="PKH58_01415"
                     /inference="COORDINATES: protein motif:HMM:NF040141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HNX87714.1"
                     /translation="MRYISGDQGSFGRLVETETGLDVKMMELPDRDNEIRMSRIPEGE
                     YLCQAYKSPKFGRCWLLRNVPGRSAILIHKGNLAGDTRKGWKTHSMGCLLPAWYKGWI
                     GRQMAGLNSTRAFTEIMNKIGHVNFKLKIIDLC"
     gene            24494..25090
                     /locus_tag="PKH58_01420"
     CDS             24494..25090
                     /locus_tag="PKH58_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87715.1"
                     /translation="MLIETILGVVTGIVGNVMTTVTNVRTQKLKNEHDLKMREFDIKE
                     REQEAQLQIAVDTAHSQNEVEKMEAEAYVKSMESANADILTDVKLKALSDNGGKVNKL
                     LMLIMGLVDAFRAFIRPGLTAYLVGLTTVITFHAISILNTKGDFITAVQAQELFTNVT
                     DIVIYLTVSCVTWWFGDRRVAKFAYRLNDGNKQDKQGY"
     gene            25090..26187
                     /locus_tag="PKH58_01425"
     CDS             25090..26187
                     /locus_tag="PKH58_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87716.1"
                     /translation="MALVTIIPSLSFSRNFPVLVVNAEEVTTVVLKQGTETVLSESYY
                     PDDSGHFEIDLADLLTSLLTVDIPQLPGEQAGALAAFTITLTETGLTAEEFSFTLIKG
                     GVLNGVEDMDAFCAGNMLTWHPQIKRVKLSDSEFITYYAVEPALVKAKAWFLSNPVIT
                     VGAVLDAGHLHVINATFSVLRAMFPAYYYPRWIDIWIENQSGAATWVQRYELIDEYDQ
                     FDDLFLFENSLGGLDTIRFTGQLTEQEKHDQESAKFGLETRDYYLDFNRVMSKNTGLF
                     QSQAESDWAREFFASTNRWWWKDGVWKKITVSEYTVERVYNDMRSYKFEFSLSQQEPL
                     RNLAREVIPDPEDPYAFDFNDDFNHDYLIQQ"
     gene            26184..27464
                     /locus_tag="PKH58_01430"
     CDS             26184..27464
                     /locus_tag="PKH58_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87717.1"
                     /translation="MTEEQLQALIDQEINDNGNGAITGPLLNSVLKSIITVIFDQVGG
                     EAAVPDSRSIKHNAGKQLAVMLPTWEPLPGSKRVKAIAFDTASEVAGGVYIKTDGKTV
                     FGNGAGELTTVNSGVLPDVTVVSDDFTTQPTGAAMNKAYLSVDSKNGYLKLDTGAAVD
                     TAYFVKKDIAVTMDGNAKFDVLFALGSLTNEKATVGLSTIDADLPDVAGIKFIFDPAV
                     DAYWHLIASDGGEITINLATLVAADTNEHLFEARVEDGKITWYIDGAEVGTTGAVNLT
                     DTELNFGVWVESTAAESKTAVIDFYNLGGDRVDEEVVTYSAQQAVSAPLKHAGFWSQA
                     GANAPTVSVLENSLGTIAIARTAAGTYTITKVGAFKEGKTVPLDDIWTDASGNVYTLV
                     WTSADVMTLTTKTSAGALADDILDNRFIFIEIYQ"
     gene            27464..28051
                     /locus_tag="PKH58_01435"
     CDS             27464..28051
                     /locus_tag="PKH58_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87718.1"
                     /translation="MSGFHKAFSRNGQYKPGNYMPAIGEIYQGGKVAFIAADESYILI
                     VAEADSDSKPWYPSAYSYVKGISNEYACGADNDALIIAEFGAGDYAAKLASYSNINKY
                     NDWYIPSVGEWQDMVQTLTEGQSLGLYNYQKGGMATASNEYWTSTNINTYSQYIYAVW
                     FDMYNIGWDATIPTPQLTKRVRLVRKLTAPFPNPS"
     gene            28539..28967
                     /locus_tag="PKH58_01440"
     CDS             28539..28967
                     /locus_tag="PKH58_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87719.1"
                     /translation="MKQLIIFALFTLLSIAAISQDKVTLTTGKVIRGKITLITNTQIN
                     ISSPSRDYECPIYQVASYETGGNVKLTDKSKLNQSSVMAEILGNELSKTSRAFAAGAV
                     LSAGGFILTTMPDQFIKIRSGDTEKEISSKTKKLKNLKYI"
     gene            complement(29093..30217)
                     /locus_tag="PKH58_01445"
     CDS             complement(29093..30217)
                     /locus_tag="PKH58_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNX87720.1"
                     /translation="MENYKLPKLYDADNDLNQRWFVFYSFRHPETGKMKRFRIFISTK
                     YKTATARREKASELIKYYSTRLKQNWNPFEQEQIQLISASAAMEKILEIKKASIRPRS
                     YFTLKNIVKSFREYLIAHKLERISINDFGSRHAIEFLDWSKSHYKLSNRTVNYRRMNM
                     KTLFNELIYREYVDVNPFSRTKKLPVEETSIIAYSASELKKISEKLHEYNFNLYIISM
                     MVFYCFIRPQEICRLKISDIDLEAQVIHLPGRTSKNKKSEVVVIPDQLKEEMEKLNLK
                     FPGDYYFCGKGLVPGPQYTAPTRIAEAWSAWAKAVKMKNRGIYALKHTSVGMVAMEGK
                     INIRDLQLQLRHHSLDMTQNYLDRFSSIASKRLKSSFPEM"
     gene            complement(30488..30997)
                     /locus_tag="PKH58_01450"
     CDS             complement(30488..30997)
                     /locus_tag="PKH58_01450"
                     /inference="COORDINATES: protein motif:HMM:NF018697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX87721.1"
                     /translation="MQTIAERLILLLTELKIKAVKLAEIIGTPTSTVSGWTTGKSAPN
                     FESLLAISNNFKKVNIHWLVTGEGEMFISEKSNYYNNIPPATAGETPITPMNSDYQFR
                     IIESLVSQNEKLQTEVSNFSAIQKDMAKAQAEMAATQRLNAETILNLSRQPEGKKIKV
                     KHRVIEGTG"
     gene            31377..31916
                     /locus_tag="PKH58_01455"
     CDS             31377..31916
                     /locus_tag="PKH58_01455"
                     /inference="COORDINATES: protein motif:HMM:NF022022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HNX87722.1"
                     /translation="MKQPLTNFLEFKGKTLLFLAKNATYWIALKPVCEALGVNYNRQF
                     QNINNDLILGPAFAVQQIQVPGDQVRKFVCLPERYIYGWIFSIQSASPELLEYKKQCY
                     EVLYEYFHGTITSRRELLKEKARLELERSAKEIKLLDNKEFIEWQQLKAEEARLGIAL
                     KRNDQDTVSEQKELFDSNL"
     gene            31927..32490
                     /locus_tag="PKH58_01460"
     CDS             31927..32490
                     /locus_tag="PKH58_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX87723.1"
                     /translation="MNFNIQQPVMKRGAIEFYVNNGEAFAEIDGQRMLLSDAPVNVHE
                     MIRRNIEHHPGAIEALESMGLNSASQQHEQYIACMYGEYNHTPDFLNFRHSVDDDEFT
                     QLLCGVNNCKFRGVLCHKIHAKYSDLTDREVQICLHIRADFSSKEIADMLDISINTVS
                     VHIANILSKIGCNSRAGIASWASTHLA"
     gene            32501..32758
                     /locus_tag="PKH58_01465"
     CDS             32501..32758
                     /locus_tag="PKH58_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87724.1"
                     /translation="MDTRYKIMIASIALIVVVIIINNIRCGRFRKNLKPGLFCRFYID
                     EDSREGIVRNIDGDTIVITDSWLNVYTVSINDIYPASIFHF"
     gene            32762..33127
                     /locus_tag="PKH58_01470"
     CDS             32762..33127
                     /locus_tag="PKH58_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87725.1"
                     /translation="MLIHIESNSMLDLLHTAVQVRLAAIQIKMSLSSTFNEQAQNSVS
                     TDIILLDELLAKIDYCIKFNIAFRSESELTANMLCDSLKTLIHYDYLDVAANHQKIKS
                     YFRAEKCKELLNGIKSRLQ"
     gene            33124..33573
                     /locus_tag="PKH58_01475"
     CDS             33124..33573
                     /locus_tag="PKH58_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87726.1"
                     /translation="MISYEIINERYSVVVALKMTDVNDHVSEFRETFLNPIDAQKWVA
                     VKLEYYLRKRISNYIIHSSMISRHSQHAYYHTAEYRKSMQELMRYLNVINDQKLHQLA
                     NYVNNNASFLEKILPAQGNPSYNSSKEILRSITNLAGKLQKRENRVF"
     gene            33573..33911
                     /locus_tag="PKH58_01480"
     CDS             33573..33911
                     /locus_tag="PKH58_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87727.1"
                     /translation="MRKGQYNAEYKPKPENTSKRKTRAGIHVTDHAIVRFLERVCNID
                     IENLKDQIVTDELLRNIERFGPTGAYPCNHENPYMVVIDKKQIITILPVSSKHYQDRT
                     TKHRAHHGPG"
     gene            33935..34005
                     /locus_tag="PKH58_01485"
     tRNA            33935..34005
                     /locus_tag="PKH58_01485"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33967..33969,aa:Met,seq:cat)
     gene            34025..34273
                     /locus_tag="PKH58_01490"
     CDS             34025..34273
                     /locus_tag="PKH58_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87728.1"
                     /translation="MELLNRKGSVDDIRCSLGFITPKTEREIKLALAQVSSSHHDEQM
                     NRKRTTVLSMLMTKYNKLIKQLQQWHQTEKSHSCAQTS"
     gene            34493..35122
                     /locus_tag="PKH58_01495"
     CDS             34493..35122
                     /locus_tag="PKH58_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87729.1"
                     /translation="MKKERPILFSTDMVQAILEGRKTMTRRTKGLAEISINAGHTQHS
                     YEGKIPTDNNIHAFARMWGGHFAETKHIKCSYGQPGDILWVRETWCNDPRGSEDGISP
                     YYYFKADFPNTDVWKGSWKPSIHMPRKAARIWLEITEIKAERLYSISREDAIAEGCGN
                     EGTDYPEGNFFALWRKINGIDSYQSNPWVWVITYKVLSTTGRPENLDLA"
     gene            35521..38172
                     /locus_tag="PKH58_01500"
     CDS             35521..38172
                     /locus_tag="PKH58_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87730.1"
                     /translation="MREMSPEDKKGEYNFTFKDHPTPADLAAIGRYVSEDILDMFNCR
                     CIEKYEYCSTSKKHNRDVVHIFKATEDYPIFLFDYGDFKKLYRPHDQEKKNRFLYIGK
                     KPKDYIYGLKQLEKAKNEFASTDEDELSLPEEKPNARVIDLFRCSGESDALNLASLGF
                     HVYWLNSESADFDYKTFRKLDDLCENHYQIMDLDPTGQDQALKNAMKHISLFTIELPE
                     WLRAKKDFRGNPCKDLKDFINMSGEDIDATRFDFLIVKNSARKVKFWTKSKDDKTGKV
                     SYNLDMEFFYFFLKANGFYIIESIYHKKAGYCYVHIDGKIVEMIHPDDIKRIVKRFTK
                     DWIKSKKLMDSIKILNKINTSNQITEANIESIDATKLKFKNHDRFTEYIHFKNGSLRI
                     TKEKIERVPHSEVPNHILGLLEINNKKLSHLVNRDIRLMEKPPVEVKATPAYQALLDM
                     LQQAATDEAREVVNVQIAQFPDIDKYEVVIHDDEFIFLRFLRDLSRIHWRKESEQKIP
                     LTPEERKEEQLSLANLLFVIGYHCAQYKDPGKPWLTFLQDMKVGDIGTASGRSGKSLF
                     SQAITYVRTSFYKGGRTLNDKSAYQFFYDGMTEFHDYIEVDDLHEYAEFGFFYTQVTG
                     KREVNPKNYTPFTLDYEDSGKMLISSNYELQNVDSSTVGRLLNCGVSDYYHEKSKFND
                     YHETRSPLIKFGRRLYDDFTEDEWVKFYNLIAYCIQLQMNFYKIQPPAGNLEKRQLRR
                     VMSQGLGRDEEFFRWANDYFILWNDSEGRRPEFSPVEHGYFNTYIYRNSPFENFQSRL
                     SKKQQSEYRTGKFKKHLEAWCEYWGYELNPEDICIDKDNRRILRTIDGKTEELFYIST
                     SKLSNQEQPKVIPLVPAQEDTDNALPF"
     gene            38181..38654
                     /locus_tag="PKH58_01505"
     CDS             38181..38654
                     /locus_tag="PKH58_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX87731.1"
                     /translation="MTHYDIDKIVARRFGITVRRLTTGTDRRKATIARAMAVMLCYDV
                     LKLSTLKLKKIYGKQAHSTIIENIQTARNLAETNRGYRTILDACRHEIEMKSSRSKIR
                     KQCYNLHYRIREKGIRIDSKQKTISVTEGEMIKISDRQTRRLIDRYHYSIQLSLL"
     gene            38651..38956
                     /locus_tag="PKH58_01510"
     CDS             38651..38956
                     /locus_tag="PKH58_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87732.1"
                     /translation="MSCGILCNCATHTDAVLRDAESFVCERSGRGIYRRRPDGMRLAT
                     MGDFANPDGSLRIGMAFLLAIEQAHTYWYVHRITKDTDRAELQKFIDSELYYVKKTK"
     gene            38958..39269
                     /locus_tag="PKH58_01515"
     CDS             38958..39269
                     /locus_tag="PKH58_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87733.1"
                     /translation="MFSEHTKHIQMATLPAGYEIVTTGNIQPGDIRWNAWEDCWNIDN
                     PVSAEKHKLIINDPVSNFHGVCRKSFQVTDSQLPENRVKENEPDVRTEYVNLEWFYEC
                     R"
     gene            39510..39962
                     /locus_tag="PKH58_01520"
     CDS             39510..39962
                     /locus_tag="PKH58_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87734.1"
                     /translation="MFVVLDNSILIHDATNIEQKSETKKKGRRTVASTAVFSRHRFEL
                     SSSDLPPLFNNQNFNVMLDKKDEMVTPCEACGQPQELTIELILKNFFQRNEAQEVKNR
                     LWEMFIGFIGSPDFDGWNAIDRSNLAFQYKELCTLVDQLEFIHLKQTA"
     gene            39965..40468
                     /locus_tag="PKH58_01525"
     CDS             39965..40468
                     /locus_tag="PKH58_01525"
                     /inference="COORDINATES: protein motif:HMM:NF022022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HNX87735.1"
                     /translation="METTALVMFSQTVKYQGNDIWIKPFCDFFGISYKWQVEVLKKDH
                     ILASMVRKNCNETLFGDKRERVLLPKKGFVRWIQLINPKTVREELQEKFKQFQELVFD
                     YLYGSAEDEEQIKVHYNRMQKLERLYSKIGTEIKREKAIVATLLNGRYTQLSLNFNTE
                     HQLTDGR"
     gene            40662..41135
                     /locus_tag="PKH58_01530"
     CDS             40662..41135
                     /locus_tag="PKH58_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87736.1"
                     /translation="MKNIFQPVDFECLLHTFVVRDILIFNEPKSKQTSFNPQGWRTVA
                     STEVVSRLSFEVSRGDHLFFFNFKSSVMRDTEDEANELMLENFDAIRDSHSRHRPELI
                     LSLFERNKPQLINDFLVEMDAKNKAYYFIIESGHFSAFSEYCLNPKKQKHNAAKI"
     gene            41119..41634
                     /locus_tag="PKH58_01535"
     CDS             41119..41634
                     /locus_tag="PKH58_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HNX87737.1"
                     /translation="MQQKFEYNNLPVRTVTDDNEQIWFAGIDICNILGYAKASTTIER
                     LEEDEKKLEYLTDTSGQQRKTWSINEFGLYSLILSSTKPEAKVFKRWVTHEVLPAIRK
                     AGKFTTEEVKEYEYSLQTLASEIEKLKDKKDEHQKCVNDLKKEIELKTLEMIAVIKMD
                     RSQMRIEFPEA"
BASE COUNT        12990 a         8758 c         9217 g        10830 t
ORIGIN      
        1 tatttgaaga attattcaag gcgtttggtg gtcgctctta aaattaccgc caacgaatgc
       61 ctatacgaac cataatggaa atcaataaca tatatgctat gaatgctaac gattacctaa
      121 taaaatttga aacggagatg cggctgatca attactgcaa tcgaacgatt gctaattata
      181 tcagccatac caggatattt ctgctgcatt tcgataagga tcctcatgaa ataaatgagg
      241 atgaaatcaa agaatatctt aaaaagtcat cttcccaggc tcagcttaaa caacgaatag
      301 gatccatcaa gcttttttac gaaaaagtga ttcacgatcc gctcaaattt aagtatatac
      361 aatacccgcg cagggagcaa acgctacctg atgtgctttc acaagatgaa atgcgccgtc
      421 tttttgccgc ctgttccaac cttaaacacc ttactatact tcaacttttc tattctaccg
      481 gaatgcgcga aagcgaggta ataaacctca gggtaaccga tattgacagc aaccgcatgg
      541 tgatacacat ccgccaggcg aaaggtaaga aagatcgtct ggttccatta tctgagcgaa
      601 cactcattac acttcgccaa tactaccaat catataagcc aaaggaatac ctttttaacg
      661 gacagttttc gcttcaatat tcggcaaaat caatacagca gtttattcat gattatgccg
      721 caaaagcggg cattacgcgc agggtatatc cacacctgat aaggcactgt tttgccacac
      781 acctttatga aggcggagcc gatatcaacc tgatacaggc attgctcggc cataaatctg
      841 taaaaacaac tatgatttat acccacttga gcaaccggct tatatcgcag gtgcgaacgc
      901 cggatatggt attgtgattt tttttaaaaa agatttcagc ccggctatgc cgggcttttt
      961 ttgctacgta catacgcaca attcgaaaaa cgcaacgtca catcactaca ttataaagta
     1021 taatttcaaa gcttcctgta aaaaaaaaca gcgggatcgt cggttccagc tttctcctcc
     1081 cctaaccccg tttattattt tttttgtaat ttgtaatttt aatagttaaa taactggttt
     1141 tctgtttctt actcaattac aaaaaaatta caaattttta ttcaaattgt aatttttaaa
     1201 aagttgtaat ttttctccat ccgataaccg gcatactatt tcaaattcca aaaatcaacg
     1261 aaaatttgta attgctcact atcagtatgt taaatgaaaa attacaacct gttttaaaca
     1321 ggtttgtaat tttgtaattt ctcaatatca atatgttata agcgtgtttt taagaaatta
     1381 caaatttttg ccgttttttt aacctaaagc agggggtatg ttttttttgt tatctccgac
     1441 atgtcggaat atgtaacctt atagtattat attgtcaata ttttttatat ataattgtag
     1501 tatggcaatt actcaatact cgacaataca gctaaaaatc agaaaaccct acttcgaaaa
     1561 ttacctgcga tgggttttca attctcagga aggcgacata aaagttaacc gcgatgaaat
     1621 cctgggcaag tttcttttct ccatggcccg cacttccgat attccgatca aagtaaggaa
     1681 ccagaccggc atcgtaaaac tgatcatgcc gtcgcacatt tgcgatgatc gttcaaaata
     1741 tcggtcaatc tactactccg ttgatgatga aaatcatata aacgattata ttgaagcttg
     1801 cgctttcatg gacctgcgca tgatggttca aacagctaag ctcgatatga agatggaccg
     1861 gaaaacggtt tttgaaattt acagcgagat gattttcgga gaaaataagt acgaagcgct
     1921 caaaaaatac gaatatcggc gtcgtaaaaa agctcttgac tatttgcgtg taacggctaa
     1981 agccttatgc atgtaagcgt aaaaaaaatt tatcactcaa aagtcccctg catggggact
     2041 tttaacggaa aacaataaaa acaaaagcca tgatcatcac ttcactctct tatgcattga
     2101 gttcttccgg gtcatggata gagctccctg tacagggtca ttccggaagc attaacgaaa
     2161 aaaatgtaac taccgaagaa gggcttgtct cggaaacgga agtaaatgca tatatagccg
     2221 gtctgagtca tgccaatgat atgatgctgg gttaccttac caggagaaaa gcaatgtatc
     2281 gtgctgccga tgctgacgga agatatcatt acatcggtac tgtcgatttt ggggcttcgc
     2341 tcgaaattga acaacgcctc gacggatcgc ctggaacaaa gtacggttat aacatacgaa
     2401 taacttgccg gtcggtggcc ggaacccaaa agcagtcctt tagatagcgg agcggctgta
     2461 gtaacattgc atcaacttaa tatgctatgt tacaaccaca cctaatttat tcaattctca
     2521 acgaaccatg ggcaatatcc gaacaggtat ttaacggata cgttcatttg cttgaaagtg
     2581 ttttaaatcc gaatatttca ttcgaaaaag gcgaaccggt tgatcctgag atgcacgata
     2641 tcaattttag gattaaaggt tctgcagccg tttcagattc aggacagtca gatgtaaagc
     2701 atatacaggt tattactatc agcggagtac tgaccaagga agaccaggaa tgtggtccgg
     2761 caggtatggc caggatagga aactggatta aagcagccga ccgcgatcct gggattgatg
     2821 ctattttgtt aaaaatagat tctcctggcg gaagcgtggc tgggaccgaa actctttcct
     2881 caattattaa aaattgcaat aaacctgttc ttggatttgc cgatgatatg gcttgttcgg
     2941 cagcctattg gatcatcagc tcatgcgcta aagtgattgc caataattca acggcaatgg
     3001 taggaagcat tggaacagtc atggaattcg ttgacgaaca gccaaagcta gaaaaagaag
     3061 gctatgtttt ccacgtagtg acagctcctc aaagtgtgaa taaacgtaga aacattgaca
     3121 agatcagatc aggagactat gaagagtata aggaaacagt tcttaggcca ataacccaag
     3181 tatttatcga tgctgtaaaa gccaaccgtg gcaatatcga cgaaaaatat ttcaccgccg
     3241 atgtattctt tgccaaggac gtgatcggcg acctggtcga tagtataggc acttttgagg
     3301 atgcactgca ggaagccgcc aacctggctg ccgaaaatgt atcagcaagt cataaatcca
     3361 cccatatatc aatgcaaaaa cccgaactca aacggcttgc caaagcagcc ggatccgaaa
     3421 cgtttgaaac cgttgacgga tcaatcacgc taactgagga acaggcaact gcagccgaag
     3481 gcgttctcga agcgcatgaa gctaaaatag ccgatcttca gcagaaagtg gaccaatctt
     3541 cccaactgca atcggaaaac aaccagctta agagcgatct gaaagctaaa aacgaccgta
     3601 ttgccgaact cgaaaaaggc gctggcgccg acagcgcaca cgttgaaaag gatactgacg
     3661 catcggatga gaattccggt aacgaaagtt tctacgaacg attcaatcgc ttaagtgcga
     3721 acaacaaaca acaataatac ccatgtcagt taccatccat caaaccctca tcgatgccgg
     3781 cgtaaagtat aagaaggatc ttcttaccat gccggttgcc gtgctgtcgg aaatccttca
     3841 gtacatgact cttcgtaccg gtttacaagg taaagaaata ggcggtgtgc tcgacactga
     3901 cgcccagctt cgcccctacc ggaccgcaaa ggatgcttcg gacaatacca cgataacgcc
     3961 ttacgaatgg gaaaccttcc tgggtgacgt tgtcaaggaa tttgacccga acgccattct
     4021 cggcacactt tataccgagg ccacgaacaa aaagccgaca gaacgcgaaa ttgcccgcct
     4081 tgtggccctc gaaatggcca agaaagttgg cgaagccctg tacgacaaca tgtttaccgc
     4141 cgtgcgaaac gcaagcggaa gcactactgc cgacctgttc aatggttttt ccacccaggt
     4201 agcagccgcc atcgttgccg gcaccctggc atccggaaac ggaaactacc aggatctttc
     4261 ctccgaagct atcgactaca ccaatgtagg cgacgtgctg aaaactgcct ggcgctcatg
     4321 cgacaaattg ctgaaaaaac gtgcagtaaa tctgtatctt cctacctcca tcctggagat
     4381 ttacgaagac tggtttcagg ccgaatacgg acatgctccc tggaataccg acttttcgca
     4441 aatgtacctt atcggtactc agaaaaaagt tcgtcttgtt cctctcgaca acatggaaag
     4501 ccaggactac atgtttttca ctatccgcga aaacctgaag gttggcgttg accaggaatc
     4561 cgataaggag gatgtaaaga tccgcgagtg cgacaatccc aaaatggttc agttcttcat
     4621 gatgagctac ttcggcgtcg gattcgatac actcgataag aggttcatta aagttatcaa
     4681 gttctcaacg gaagctgctt cctaatctca ggtaatcatg gcagaattat ttgaacaact
     4741 ggagtggctt gacgggcaga tcaacccgtc aggcatcaag aacattgtgt acttcgctcc
     4801 gaaaagctgg atcaaaacga taccgcgcgt taccactcct gtagccacat caactgagaa
     4861 tgttgttgtg tcgggagatt tcatcatgga atcaggtaaa acattcctgc gcctgtattc
     4921 tactcaggga aaaggaaaag tttcgtggga gccttctggc gaaaaggacc ataagatatt
     4981 ccttaacaaa gggatattct cttttcccga tatcagtgta gctgccaggt ctatggccaa
     5041 acagcttatc aattccaacg tggtgattat tgttccgttg ccgcacgaaa ccgaaaagcg
     5101 ttatatcatg ctcggcgatg ccgactatga tgttaccgta tcgattaaag gcgacagtgg
     5161 ggataaacct ggatcagaaa aagggatcac atttgaagtt gaagctccct gcactacccc
     5221 tcttcctggt tatgctggcg aacttgtact ccctgacgga acccttgatt gcgaaaccgg
     5281 cgtattcacc gaaacggcaa gttagtgaat tgcgctgcta atttcagacg ctgtcatggg
     5341 ttaatcccgt gacagcgtta ttgttttacc attaatctta ccaaaatgac accccacgaa
     5401 gaaataacag catacctcga aaaacaggaa aaaacgttcg atgaaggttt ttcaattttc
     5461 aggaaatatt caagaaataa aagcctggaa catttccttg aaaggaagcg cgacatggca
     5521 aaattggcct atgaactgag gaaacttctc ggatcaaaaa taaaagacag tactgtttct
     5581 acacttaatc tgataaaatc acagccggcc ccggccgtga atccggtagt tcctcccggg
     5641 cctgatggcg ccaccttcag gtatctgcgt tttcagaaga taaaccctga agaactgccc
     5701 gccgatgtgc gccggatata cgatcagatt gcagaagcat acaaaatgca gcgcacatgt
     5761 cacgagaaaa tgaaactggc taaaacagac aaaactcggg ctgattggcg caaaaaggtt
     5821 gttgaattcg acgacgtcat atcttccgga tggaacggaa ttgatgcttt cgtagctggt
     5881 agcgatacca ttccagatac cgccaatgcc ggtaatatca ccgatgtaag caaagaaata
     5941 aacgcctgca ggagttacat atctcgggcc atcaacgatg tgccgaaact gtccagcgac
     6001 aaacgcgaaa agcggattaa ggagacacag cagcgtttat ccaggctcgc cgctctgaaa
     6061 actcctataa cgtccgaaac aagggacagg ctgatcgaag ccggtatcat cgatttggat
     6121 tatcaggtac ttctcgaatc tgcttaatag ctgccgcatg attaacggtg aaaaggtcac
     6181 attcaacatg gcgacaatgc cgtcgcgtgt cgtcgcatta cgcgatacaa taaatacgat
     6241 ccttccgcag tgtgatgagc tcaatcttta tctaaataat ttcatcaatg tacctgagtt
     6301 tcttgggcac cagaaaataa aactttaccg tagccaggat gagtggggcg acatcggcga
     6361 tgtaggtaag ttttataggg tgaacttgca acaagggtac atttttactg tagatgataa
     6421 gattatttat ccagcagact atgtgcagca gatggttaca accatcgagc gcgcccatcg
     6481 caaggctgtg gtaagcaatc acggaaggtt atttcataaa gataagccta caaaatcata
     6541 ttacttcgac acggcaaaga atttcgtgta ctcattggcc tatccgctca cttttgtcca
     6601 tgaaataggc accggtgtgc tggcatggca cagcgacaca tgccggcccg acatgagctg
     6661 gttccctcac accaatatga ccgatatata cttcagtatt gaggtgcaaa agcgtaatat
     6721 cccgatcatc attcacccgc acgaaaatgg atggttgaag ttgggaacca agcatgatga
     6781 aaattacagc attcatgcta attgtaaccg taaggataag tttcaaacga gtgtggttaa
     6841 tgcttttagc tggaaaatta acacatgccc ggtcgatcaa ccggccatgg gcaagtctgg
     6901 aaaagatgta acatttgccg gcctcgattg ccggtaccga gttcctgaac tagacctcgc
     6961 catcgtgata ccggtctata atcaacttga attcaccagg aacattttta aatgccttcc
     7021 gctcactagg agtaaattca cagtgatcct catcgatgat gcttctaccg acgacacgcc
     7081 aatattcatt gatgaaatga tgaaacaatc ggccatgtcg ataatatatc gccggcatga
     7141 aacaaataag ggtgtcaatg catcatggaa cgaaggtata agaatatctc gcgccatagg
     7201 cgcaaatcac attgctgtgc tcaataatga cctggagctc ataaagaact gggatatgcc
     7261 gatgattgag atgctaagtg acgcaacggt aggtattgtg tgcccatatt ccacgcatgg
     7321 taagtcattg ccggttaatt tcggcacgta cccgggaggt aaaaaccatt tggctctcga
     7381 cattcttggc tgttgcttca tgtttcgccc cgatcttatc gataccatag ggtatatacc
     7441 tgattcattg gttacttatt atggtgataa ctggttgcag gaaattaccc ggatggccgg
     7501 tatgaaggta gtttatgcgc ctggcagccg tgtacaccac tacttccagc aaacaaccag
     7561 caaaataagc cataagcaaa ttttggcgga cgacgccgaa gctttcaata agctttgtgc
     7621 ctccggacta cttaataaca ttacacttta gttatgccaa taggatctgt agtattactg
     7681 cctcacgtga tcagtgaggc tcagaaaata aaaccctcat gtatactgga tgtaggcatt
     7741 ggttatggca tttatggccc cgcatttcgc cagtggctcg atatgggtgt aaagccttat
     7801 aaaacgatag ttgacggagt cgaaggattc aggcagtata agagcgcttg ctgggacgaa
     7861 tataacgatg ttgttattag cgacattcgt gattttactt ctcagttcac ctatgatatg
     7921 atcatttttt ctgacgtcat agagcatctt actaaggagg atggtctctg tgagattgaa
     7981 aagttgaagc gcaccctgaa aaataacggc gttctgctca ttggcacacc ggcgttatgg
     8041 atacctcaaa cggatgtgca taataatgaa tttgagcgcc ataaaagcct gtggacagtt
     8101 gaagacttcc cgggatttga ggtgattctc gatggatctc ctgtgctagg caacaggatg
     8161 atactggtaa aatatacaaa gcatgaaccg gggacctaaa aagaacctgg ataagttcca
     8221 ggccatcatg ttcgacgatc tggcatcgct cgatcatctc aaccaggatg agaaagatca
     8281 gcttcgccgc tatcgctttg catttaccag gttgctcgat aatccatcga tgagcgacgt
     8341 aacgcttcgt gatgagctaa tgtctcaatt tgggatcaaa caaacccagg catatgctga
     8401 catttctaat ttaaaaatta tccttcctaa tatcaggaat gccggtaaag aatggatccg
     8461 ttacgtagtt aacgaagagc tgaaggatgc tattgcacaa tgtaaaagtt atggaggtga
     8521 taagcttaaa gagctcatac tggcaatcga taagcttgcg aaatataata aactcgatca
     8581 aaacgaaggt gaagaaatag attgggatca gatatatccg caacctatag aaccaaccgg
     8641 agatatctcg gtgttaggtg aaatcccgat ggaaaacaag cgagaaacga tagaaaaact
     8701 gatcactaag tttaggaatg agatcgaaat tgaagacata gaatgtgaag atgtgagcaa
     8761 tggagaacaa gaaagtatat tataacgata aacagcttga atttcgatat atagctgctc
     8821 acacatcggt gatcgttggt ggacggcgtt ttggtaaagg acatggaata aatgctccct
     8881 ggttgctccg aaatgttcaa tacatgcccc ggagcggagg cggtatcgtc ggatccacat
     8941 tccagcaact tctcacccgc acaattcctg gcacgttaaa gtcactcgaa gatatgggct
     9001 tcaggcgcaa tatccattat gtcataggcc ggcggcctcc cgagtctctt ggcttcaaag
     9061 atcctgttat aaagcctgtg agctacgatc atgtgataag ctggtacaat ggttcggtga
     9121 actaccttat cagccaggat gttccaggga gttccaactc gctcacactt caatatatac
     9181 tcgccgacga agctaagttt ctcgatttcg agaaaatgaa ggatgaaaca tttccggcaa
     9241 acggaggata caaaggtccg tggtcaaagt gtccgtggct gaattctatc ctcatcactt
     9301 cagacatgcc aaccagcaaa aaaggatcct ggtttcttaa ttataaggat aaaatggatc
     9361 cggaggtgat tgactcaatc aaatggctgc tgaaggaaat aaatcgggta aaaaatctca
     9421 atacacgtaa atataacgag gcattgaagt tttataggct caggctggca cagcttcgaa
     9481 gcatcgcagt gtactatcgc gaatggtcaa ccatcgaaaa tgttgaactg ctcggccaga
     9541 agtatatagc acaaatgaag cgcgaccttc ctcccctggt attcatgacc tcaatcatgt
     9601 gcatcaggcc cggtaagctt aaaggcggat tttacccaaa ccttcgcgaa tcggtacact
     9661 attacacagc atatgataat agctatctgc aaaacctgga ctacgattta gataaggcgc
     9721 aggatatgga ttgcagccag gatgccgatg tagacctgtc gcggcctatt tgtgttgctt
     9781 tcgattacaa tgcaaacata aattggctag tatgcggcca gcagcgcggt ataaaagcat
     9841 tggtacttaa gtctttttac gtaaaatacc agcgaaaact tcgcgaatta gtcgatgatt
     9901 tctgcgaata ttatcgacat catcacactc gtgaagtcat ctattactat gacaacacag
     9961 ctcttggaag taattatgcc gtgagcgacg aagattttgc atccgtaatc tgtaaccaat
    10021 ttgaaaaaaa taagtggacc gttaaaaggg tacatctcgg gaatcctctc aaacatcatg
    10081 agaaatataa tataattgat caggctttta aggggcagaa gtatctgatg cctcaatata
    10141 acaagccaaa caatgaagcg ctacttctcg ggatggagca ttgtggtgta aaaataggtc
    10201 cgctcggttt tcaaaaagat aagagtggcg aaaaactggc cgaaaccgaa gaagatcttc
    10261 ttgaatatcg taccgatggc accgatgcac aggatacttt actgctcggt atgtttttca
    10321 atcctgataa ttccacaggg gtaatgatgc ccatgagcgg atctgtcatt ttatagaaaa
    10381 acagtttttt ttattcaatt acgcagtcta aaaaccggtg cttgtaattc gctaattaaa
    10441 ccggcaacaa attacatatg agtataaaac ggtactgcat acgtgattaa tttccgactc
    10501 aacctctgct tatcccgcac cgcgctgaat gcagatgtaa ttacgaaaat ttgaaaaaaa
    10561 cggtatatgc agcgcttttt tgaaaaaaac tacactctga aatgtccttt acttaccaac
    10621 ttaacaggat tatttttgat ttctcttaca attaaccctt ttactactaa ccttttaaaa
    10681 atgaaaaaaa tcttatttgt catgctgatg cttgccatgg tggcagtatt ggcaaggggt
    10741 gcgcctccta taaccggcga cattatgaaa tacaaaacag aactttcggt gcaaactgtt
    10801 tgtaatgatg cctcgatctg cgttgtcaat ggatgtttca tttctgccat atcctttaat
    10861 cttcccaggg atggatgcca gcaggtagac tgcatatgtg cagccattgt tcctaaaatg
    10921 agcaatgtga taacctttga taactatact atacgagcga aaggcaatat atggtcgtcc
    10981 ctctctatta caagagatta tggatacatt gaggccccat tgcggttaca cggttattga
    11041 atcacatagc atgatgttaa aagaagcagc tcaaagtagc tgcttctttt gtcctttata
    11101 atgctgatgt aatgagtttt ctttgtaaga aaataataag ccatgatcga tctcactaaa
    11161 gcagtcaaca ataataaatt cctgcgcgag ttatatggaa ttttcgcttc agtaacaggg
    11221 tatgcccctc ttaaggcaca gagcgttaac agccttggca atacaaactc aggcgtttgt
    11281 ggcggatgtg tgtcggtgac aacaagtgaa acagcattcg attatccttc atttattgaa
    11341 tgcaccggaa atgatggcac gattgactat acagatgagt atggcaatgt tgttgccggg
    11401 tacccgatga cgcagggaaa gacaacgaat tttcgtgtca ataaggtgac tgcagcaact
    11461 gccacagggc tttataggtg ctatgctaag taacgggcca tgggagtcgt aaagaactta
    11521 tcaatggcgg tagactgccg cgcacagctt ggctcaaatg tgaacctaaa tggactgaaa
    11581 ggattgatgt ctttttggtc ggattcgttc aattttacta caaataaatt aactgacaaa
    11641 tccggcaatg ctgacaatgc gccatcattg gttgatagta attgcttggt atttaatgga
    11701 acttctactg tggtaacaat ttcaggaact tatgtgcaaa acattgctaa agttttaatt
    11761 tatgcgcaat ctggctctga tgcgtggact atatttactg aacttacagc accgaatggt
    11821 ttgtatagta tttcaggaaa tattttatat attgggaaaa atggagctgc attttttgca
    11881 ggtaaaattg ctcgtataaa atgttattca agtgctgata gacttttggc agatatacca
    11941 ttatcaatgg gttacggaac aaaagtctat ggattagtac cagacaacaa ctttcttgat
    12001 tcaacagtat tatctgcaag tttggaaggt acatttatag atacaaacgc agacggagta
    12061 ccagacccac ttattccttc aactaactgg acaaaagtaa cacaaacaat agtaaatgac
    12121 gcaattaatg gatggagtgg caggcatgga agaatagatt ttacaacaaa aggagatggt
    12181 acttattatt acttgcaggg tggagtaaca ccaggggaat tgttaacggc tggttatttt
    12241 aaaattagat ttagaacaaa tataagttca gctaataaaa tttgggtacg ttatggcaat
    12301 tatatgacaa ctgtatctca atatttaaaa tatccagttt ctggtgcaat aacagaagga
    12361 gagatgtatt ttgacgcaag taattgcgct acactaacaa tatggtttaa acatactgaa
    12421 gaaaatacat actttgaata cgattttatt cagttacgaa aatatgctgg atatggaatt
    12481 ataacagatg gtgtatggtc agcaacatct ccaaatgcac actgtaattt agaaatgggc
    12541 gtggatgttt atgttaatga aacgaataaa atttatgtcc ctaaaatcaa ttctgaaaaa
    12601 tctcattacc caggtacttt aacctttcta aagtcgtatg cacctgctac aaattcatta
    12661 attgatgttg aaacaaaatt acagtttcct gacgtagaag ctttaaaaac aacggattca
    12721 aaaaattatc tgtacaattc gggtggaacc gctttaaaat tagggtacag cgatttagtt
    12781 ggcgcgaaag gtgattattt cttcagcaat gtctatacac caaaagaggg gataaatgat
    12841 atcgttttgc ttaaagaaaa tgtgacaaca agaaatttaa gaacaattaa tacgaatctt
    12901 aaaaaaagcg ataaacgaat ttatgtgaaa tatatttcgc cttatttgta tatcaataca
    12961 aaactttcat tcggaaaaca tttgtttaca aaagttccac tcccctcgct gcaaacgaac
    13021 aatataggcg aaatgtatct ggaagataca gaatataagc tggctataaa tagcacttca
    13081 agcctattga aaaactgtac ggacgattct gctcccgtaa atgtaaatgg ttcttatgta
    13141 ggtgggaatc atgcatacgc aggaatgatg aatgttacat tagttggtca cgataaagat
    13201 gcttctgata ttggttccgt gtggtcaact ggtggattta attataaact cgttaaaata
    13261 gtaggaaatg ttttgtcgtt catccagtct gggactgcaa cgcattatgg atataaatat
    13321 aaaaccactt tttcatcagg aacattaata catgttagcg gggcaacaca cacaggcgat
    13381 ataagtgcag caacaacagt accgaatcaa tatatttctc cgggcaaagt aagaacatta
    13441 acaaacatac ttgcagatgg attggttatt tcagatggtt ttgatggata ttgtaatgag
    13501 ctggagattc aaatcattga aaatatttat aattatctgg tcatagaaga ttattaccca
    13561 aatgacttgg caaattgtcc gtttattatt cgtagaaatt taagttatat atatacatca
    13621 gatggaagta tgagaacgaa agaaacggta actgcattac ttgatgatgt atatttctcc
    13681 gggcaaatgg gaattgatca ggcagcacct ttaaattcgg caatgtcagg agtaacaaaa
    13741 caatatttct atttacccgg atcagcacca ttcacaattg gatcaaatgc ttatgattta
    13801 cgccagatgt cagattggtt ttcatttact cctgatacca tattattcac tcccgaaaaa
    13861 agagaaacag gaaagcatat taatagaatg gttgaaataa tttcttcgga tgctgaaaaa
    13921 atgtatggat tttcacacgg atatttttta agagataact ctgttaatgt tgctgatatt
    13981 gattggtatc tggttaacac gaaaaaatct tatccggaat atgctacaaa tattgcgtta
    14041 ttaaaggata cttcaatttc tggtgaaagt tttagaaagt actttgatgc acttcgtacc
    14101 gattcggcta cctgcgctta tattgtcaag gcagattatg gttataacct ttatattgac
    14161 gttcatacaa atttgtcaga tgcgtggata tcagtaccgt cagaggtagt tggaatgaac
    14221 atagcaattg atgatagcaa cgaaaatata acaatccact ccacgcaagt tataagtgac
    14281 ggaattaaag taacggtaat caataattac ggttatgcgg ttttaaggtt aacctaaatt
    14341 agttcacatt tggaacgaca tgaacattat aactatctca atagcagcct tgatttacct
    14401 gttttaccag gccgctgacg cgtggggaga cgtggctatt gagctcggcc accaacaacc
    14461 ctggcacacc tggggcgcgg tacaggcggg gatgttcatc gcagtgcttg cttttgcgct
    14521 gtgcggatgg agctggtgta cggtaattgt ttcagcctct ttggtagtgc tgcgtttccc
    14581 gattttcaac cttatgcaca acgccgctaa ggggcaaagg tggtgttacc tgtctgacaa
    14641 cggcattgat ggcttaatta aaaaactttt gtcatggctg acataagtac acttcaagtc
    14701 ggtgatatca tcctggtaag ggatggtaaa tcatttattg ccagtggaat aacatggtgg
    14761 atgaaaattt ataaaaaaaa gaaaggaata acactgcttg caacctatca tcatgctggc
    14821 actgttatta cagatgaaag tgtcctgaga attgctgaag cggttggaaa gggatatcag
    14881 atcaattcga ttgaaaaggc gtattcactc caggaatggg gaaaaagaat agatgttatc
    14941 agccccgata ccccttatac tgaaaaagaa aagaaatggt tgtcacaaaa agcaaaggaa
    15001 tacaaccagg agatcaccag gtatgacttt ctgaatttct tcttccagat atggttgata
    15061 aaaacgggga agtggatagg accacgggga aagaaagcag agaacaggtt atattgcagc
    15121 gaagcagtgg ctacaattgc caattatgtg aggccgtgta cgtttgttaa tcctgccgca
    15181 acgaacccga tggatattgc cattaatcac aactatcatt tcatatagat ttttttgtcc
    15241 tttaacaatg cattctgctg tactatcatt gctatgttaa ttacacagca atgattcttg
    15301 caaataacct tcataaacta gtcgaaaaag gcgaattctc tatcgccttt atagccgaag
    15361 aaggtaacat cgtgacctgc gaccgtgcta tatgcacatc ctggcacagt tcaggccgga
    15421 ccatgaacat aaaattcctg gtgtcgaaac aaatacgaac gatacgccgc tgcactatca
    15481 ttgcctttaa tgatgaggaa ttagtattat gacgacagat acatttgtaa tcgataacat
    15541 tacaattatg ccagggctgg ccgctatcat caccgaatcg gacagccagg atttaattga
    15601 agatccgacg aatacaattt caatcggaga aaatgataaa atagcgccat ggggacgcga
    15661 taacgatctt cctgataaag tcttcgcaaa aattatgaag tcggatgtgg cagcttcaaa
    15721 tctgatgttt aacatccagg cttgctacgg gcaaggaatc aaaccgatga tgcgaagaat
    15781 tgaaaataat acggtagcat accaggaatg caacgatgaa aaagtgatgc agtttttcga
    15841 tgataacgac ataaatggtt attttcacga gcaatgcact gattttatta cattttttaa
    15901 cgctttccct gaaattgttt taacggccga cggccgatct gtgtatagcc ttcgtcacaa
    15961 agaggctatg tttagccggc tcggggttgt caacaataag ggcgaaatag taaagcacta
    16021 ctattcgtcg aaatggaaca gcgaaagcgg atcggctaac aaagagaata ctgttataag
    16081 tgaagtatta tcgcactata atccatatgc tgaccttaca gcacgaataa cgaaaggtaa
    16141 ccgaacgaga cgttttatca tgccggttcg atttccggta ccgggtcaca tctactaccc
    16201 taaaccatac ttctggtcta tgttccgttc cggatcctac gacttcagca gccttatctg
    16261 gaagtataaa aaaacgctgc tcaagaatgg attggccgtt cgttacatca tctacattga
    16321 cgaaaagtac tggcaaatca tctttgatga agaaaagatc gatcgtaacg atccaaagaa
    16381 ggtgaaagat aggaaggagg aagagtttgc taagttccga aacttcctga ccgacgaaca
    16441 caaccaggga aagggactga tggccttgaa aaagaccata caaacaggta ccggtgcgat
    16501 agtggaaaag tacatcgaaa ttgaggaagt aaaaataaca tcgaagggcg gtgaatttat
    16561 cgaagattca tctgaagtaa ataacgtgat gagttacgcc atgggcgttc acccgaatat
    16621 gataggggct aacccgggca aaacaggaca gagcatgagc ggcaccgata aacgggagct
    16681 ttacatgata aaaactgcca tgatgaagcc tttccgcgac cggttgttgc agccgcttta
    16741 cctggttaaa cgctataaca aatggcctaa tgaacttgaa ttcgttgtta ccgactacca
    16801 gtttccaaca cttgaccaaa acaaaacagg aaaacaggag gttgcacagt catgattata
    16861 acaacaatag cccaacttaa agatcgtatc gcaaccgtta agggagcaga tataaaaagg
    16921 tatgaaccat acctggcaac tgccgacctg ttcctgcaaa gcgaactgat tggcagtacg
    16981 ctttatggca agcttaacgc gaccggtaat gaaacattgt tgacgcgctg ccaggatgtt
    17041 gttgcgctta aagcatatca tgatgctatt cctttccttg acttgatcga gacagagaca
    17101 ggctttggtg tcgtttcatc tggcggatca aacctggtac cagcctcccg cgaacgcgtt
    17161 gataagctga tcgctcagac agagatacga ctgtcgggtg ccatcgaatt attgcttcag
    17221 catctggaag aaacaactgc ttatcacact gattgggcca cggcatcagc ttacagcatc
    17281 attcacgata gctatatatt cacgctgacg gagttccgca gatatgcccg gtatgatgca
    17341 tcgaggctcg aatttgttaa ggatgctcct aaaatacaga gggcaatccg atatatcatt
    17401 gagcctgtta taagccggga gctgagtctt gctattatta cccagttgaa ggccggcaat
    17461 cttaatgatg ccaataaagt tattatcgaa gatttgcggt ttgctcttgc cttatatgtt
    17521 acaggagaac cggtggctgc cagtgcttcg ctggcaaagg ttaaagatgt attgtatacc
    17581 aatcctgatt catacccgga gtttaaagca agtaacattt ataccgcctt cctggcgcag
    17641 gaagaacgcg acacgcattt agacccattc atggtatgcg gaatgtaaca accataaacc
    17701 tgcaggctcc aaaggcgtgg aatgagctca gcggaagcca gctcaatttt attgccaggc
    17761 aattgatccg aaaagctgaa gctgctgaga tcatggttaa ttgtttcctg cagtttaccg
    17821 gccttagcat caaaaagaaa tatccatacc aggataataa cggggtatgg taccggtttc
    17881 gcaaacgcgg cgtcggatcc ttcgacctag atgccgatcg ctttacatgg ctcgctaaaa
    17941 agctcgaatg gatcactgat gatataagcc tcttccataa tcctaaacgg atattatggt
    18001 ttacaggatg taattataag atgtatggcg tgagctttga acgatggttc ctgctggacc
    18061 agtactatgg agcgtattgc cacagccgtg atcaggaatc gcttaataaa atgatagccg
    18121 tatgctatcg gcgcaagggc gaagcctggg acgacggtaa aaacatggat aaacgcgcct
    18181 ggctattccg gtttgttcca ttgtaccgaa aatatgtagt ttttctttgg tatacttcgc
    18241 ttaagaaata tctcagcttt aaatacccgc atgtattcag cagcgattcc ggaggagtaa
    18301 cggtaaagcc ttcggcaaac gaactcatta tgggcattac ttcggcgcta aatgacggta
    18361 atattacctt gaaccctcag atcaggaaga ctgatgtgca tgaggtgctt tttgaactga
    18421 accggaaaat tgaatcttca ctaaaaatat aatgccatgt tcgcgcttta tgaatatcta
    18481 gattctgtaa aagcagcact atcaatcgat aagatatata ggatatcagg catacttcag
    18541 cttgaggaat tcctgaatgg cctgggtgat gctcaggata ctgtcgtatt tgcccgcgac
    18601 agcggcgatg gttaccttaa tataaacgac cgcaggctcg acaccgcata ccatatgttt
    18661 tacgtatttc aaagggctga aatcaacgat aacgattcgc gcctcgatgc caagcgaaac
    18721 agtatggcta aaggcattac tatcgtcgaa aaaataaaag cagagagtaa agactttggc
    18781 gatccggctt atggtttcaa cgcagcccgc attgactatt ctgaaatagg gcctattgcc
    18841 cgcaattatt acggctattc gtttgccttc ataatggaaa accatttctg atggctgaaa
    18901 acataaatat taacctcact gttgaagcct gggctgatat cgtgatcatg gagtggatta
    18961 aaaaatttga ggctcttggt atacgtgaac ttcatggtat tcacagtttc gaaagctcaa
    19021 ttattactgc atccgatggc gatcctgcca aggtgcgttt cgcctttgag tggtacctta
    19081 aaatggttga ctatggagta ggtaaatatg tcaatctgga aacacgcgac accatgattg
    19141 ctgccggcct cactacaaga aggccgaagc cctggtttag cgataccttt tacaaacagc
    19201 tatcggtact acaccactta tacgaagaaa aatacgccaa aaaaacagag ctttttattg
    19261 ttagaaacct caaggataat gctgatttag gatttaatga agtagcgcta tgagtaaagc
    19321 cattatagat gtcaccctta ataatgaaga agcgaaagca agattgaaag agattgctgc
    19381 agagctgact aaagttaaaa atcttcgtga acaagcgctt gaaaaaggcc atgcatcggc
    19441 ttttgacctt gccaacaaag aaatgaagaa gttgcaggcc gaaagcaaaa aactcagcaa
    19501 ggaggttttc gacgtcaata atgttcttaa gaatatttct tcagcatcta tcaaagacct
    19561 taatactgcc atccggactc tgtcgcgaga tctagataaa atgaaacgca ccgatccggg
    19621 atatggcgaa aagcaaaagg cccttgaaaa tctaagggaa gaatataaca gagctaccgg
    19681 aaaggcaaaa gaacataccg gtgtgcttgg ccgcatgggg atatcgtttg gaagcgcaat
    19741 gtcaatcgtg ggtcagtttg cagcgggact tggcattgca accggagctg ttgcagccat
    19801 taagggtgtt attgcatcaa ccgatacagt gagcgaccgt ttcaataaaa cggtcggtgg
    19861 acttacgtcc gggctttcgt acctcggacg cgccatcgca acgatggatt tttcaaactt
    19921 tctttccaat atgcgcagcg ccattgagga gggacagcgt tatgtggata cagttgacat
    19981 gatcggcgac aaaacaagag ctcttacgat tatcgaatcg caaaacaggg ccgagatagc
    20041 aaaaaataaa attaccgcca gggatgtcac gctcccttat gacgaaaggt tgaaagccgc
    20101 caataaagtt ttggatttag aacaaaaaaa tgccgattat aggaaagaga ttgctgatga
    20161 tgtactccaa aatgaactag atagcgccaa agacataaca aaattgagcg gagaacgtat
    20221 cgaagggtta ttgaaagaaa aacttattaa taaggataat gtggccctag caaatcagta
    20281 tctcgatgcg ctcaacgaac aaaaaagagt agaaaagcag tcagccagca aggttgttgg
    20341 cgctccttat tatgtacaag caggggctgg aaccgctctt tcggaaaaga gtgcgaaaat
    20401 attacgcgaa actccagagg atataaagaa atatgcccag gaactgaagc agttcggcaa
    20461 aattgctgaa gataccagta aagattcgga gacatcgctc aataaactta ccaaagcatt
    20521 tgaaggcgtt ggcgaagctc aggcttatta tgatgaaacg acttccagaa ctcagaagaa
    20581 caggtcaacg attataaagg agatgctcaa ggatgaagcc agccgtgaaa aaactatcaa
    20641 aaatcaggat acagcgtatg aaaatctgaa taagcaaatc caggaacttg agaaaacgct
    20701 tgccgaccag gtgttccgaa acgatcctaa atcagcccaa acgctaaaat tgcttgagct
    20761 ggctaaagcc aaagctgaag aagtaaaaga gatactcgat tcatataatg ccgcggctga
    20821 tattaaaata tcagaaaaac ccgatcagta ctttacagcc ggattcgacg atgaaatcaa
    20881 tgccattatc gataagaacg acttggagac taaacttctc gataaaaagt tggacgatca
    20941 gcaaaagatt tacgaggatg cgctcaaaga caaagaaaag gccgataagg actatcaggc
    21001 aaagaaagag gatatggaga agttagctat cgatacatct atatcattgc tcaatacagc
    21061 agccgacctt atctacgaga aggatatgca ggctatcgag gataagtatt cggaggagga
    21121 agcgcgtttg gagaaaatgc tgaataataa tagtatcagc cagcaatctt acgaaaataa
    21181 agtaaagtta cttcgcgaga agcgtgcgaa ggaagaagag aagattgaaa aacagacgtc
    21241 tgcgatcaag aaaactattg ccgctatagg cataaccgcc gatgctgccg aatcggtctt
    21301 taagattaaa gccatggctg cagagctcac tgccaaagcg gcggtacttg ccatcaccaa
    21361 cccggctgcc gcggcattat acccgccgct tatcgctacc gtattggcac aaatacccct
    21421 tattcttggt tctgctgcgc tgcagattac cgccgcaatg gcatcgggcg gaaagaaagc
    21481 cggaggattt gccgctaccg ataaaagcga cgatacgcca atgggctatt atcacgccaa
    21541 tgagtttatc ggcaatgccg attctgtaag gaatccatct gtgcgcaaga tttatgatgt
    21601 cattgattac gctcagcgca acggtactat ttcgaacatc aacctgccgg cgattatatc
    21661 agcctcattc ccgggacggg aatcgggagg gtataccggt cttgatgatg acttaaacac
    21721 gttgagtgat gcaaaaacaa aggcatggat ggcgcctgga gtatattatg ccgtaattgg
    21781 cgatgtatta aaagaagtag cgctcgttgt aaaagatctg aagacgcggc ttgatagccc
    21841 tatcgaagca tcagttgcgc tcaggggcag caaaggattg tacgaggcaa tggacgaaga
    21901 taatcaactc aaaaacaacg cttcgttatg atcgagatca tcgttaataa tacaacccga
    21961 ttgcagcttc cgtccaaagt taaaatcaac attgttcaga aaaatccgct gtttctcgaa
    22021 gaccgtatac cctcgccgta ttcattgtcg ttcgaggtac ccgttacggc cggtaacctc
    22081 gccgcaatgg ggcaacctgc gcgtatcact tcatcgtcga tcaaagcgcg gcagccaggt
    22141 aaaataatgc ataacggtat ggtaataggc gccggtgaaa ttctgttgct tgaggttaat
    22201 aaggtgctga aattgcagtt taaggggagc ccacttaacg gcaattttaa taaaaacctt
    22261 aaccagataa ccaacgatga atatgactat ggtaccatga cgtaccatgg agaagatatt
    22321 aattattcag atacttcttt cgatgcctac gtgcaaaatc ttcgttccga agctaataat
    22381 gcaagtcact ttgtgcttgc tccggtaaaa ttggctgata cggaatggaa tggcacttct
    22441 tcttacctag gcgcgaagaa cggtttgatt aattatctta atttctggaa tgtagcaacc
    22501 gcgtcatggt ttaccggcga cgttcactat atgcacatgc cggttgtgcc tttcccatac
    22561 ctcaaagata ccatcgcgaa agctttcggg tcgaacctcg aaagcaatcc attcgcttcc
    22621 ggcgaccttg ccaagctggt taccatatca gcaaatcatc cacacctgaa tttaaatagc
    22681 ctctatgatt ggtatcttac cggacctaac ctcgaaacaa aacaggaagt attttggcct
    22741 ttgctcgata gttatgtttc ttccaattac ctgataccgc tgaagtttaa tatcatgaac
    22801 ttcatgcaag cttatgcttt taacgaattt ttgaaagaga ttttaaaaat gttttcgatg
    22861 tcggctttcc cgggcatcca gtaccgcatc gaaaagaata atgatatttt cgatcggtcg
    22921 gtagtcgtca actggtctga taagctggcc ggaaagccta tcattaaaac aagggatgca
    22981 aaggactata actttaaatt cagaaatgcc ggtgaaaaag catctgatcc ggtaagaact
    23041 aaaccgaata tcgatgctat ttacgactcg gcacttgaac agggtagtga ggaagtggaa
    23101 tattccgatg ctgttagcgg agctatcatc ggaatcacaa aagagttatt cggagatgcg
    23161 agcgaaccaa ggcttacaca agacacaaag caaagcgcct tatctgcaac tatagaagat
    23221 accggcctcg ataaatatga agtgaattgt gaagttgagc cgctacctat gtcgattgaa
    23281 cagtactggt ccgataacga gaatcctgga aatgtaataa gccataaaca ttggcatgta
    23341 cctgtaattg aaaagccagg tttgaatgat gccccaaaca taatgatatg gggtggcatg
    23401 gccaatactt ttgaaggcga cggaacgtac cctcttctga tgaatcatca tactgatcat
    23461 tttggaacca agaggggcag cctgtcgctt ttgcccgatg gccccgatgg ccttatcagt
    23521 acttttcact cgggcatgaa aagctggatc gaatgtgaaa aaaagagcgt gaaaggttct
    23581 tttcgcctct cgccgttgga gattcgcaat atcgatatgc gcgataagat ttacttgcat
    23641 gggcgcttgt tttacatcga aaagattgag tattcgctca cgcataatga tgtaagcctc
    23701 gtggaagccg atcttattga gtgctaaaac ttttgtcctt tgacaaaata acgtgacaaa
    23761 ttatgtttgc taatcaaaat cagatcaacc atgacaccct ttgaaacctg gctataccgt
    23821 ggcataattg cagttatttt actaatcgtt tggtatttta ttcaacgatt tgtgaataat
    23881 gttcaggaca aactcgatga tatacaggat ggcattaata aactaaacaa tcagagcgga
    23941 gtgcatgaag aacggctaaa agctatgaat atggcaattg agtctcaggg ggaacggctt
    24001 aacgatcatt ctcaacgatt gaggatggta gaaactaatc aggcaaaatg ccgcaattgt
    24061 aacaatgcaa ccaaatgaaa actgctgttt taatgcggta catatctggc gatcagggca
    24121 gctttggacg tctcgtcgaa accgaaacgg gtcttgatgt aaagatgatg gagctccctg
    24181 accgtgacaa cgagatccgg atgagccgaa ttcccgaggg cgaatactta tgccaggcat
    24241 acaaatcgcc aaaattcggc cggtgctggt tgctcaggaa tgttcccggc agatcggcaa
    24301 ttttaataca caaagggaat ctggccggcg atacccgtaa agggtggaaa acccacagca
    24361 tgggttgcct gttgccggca tggtacaaag gctggattgg acggcaaatg gcaggtctta
    24421 attctaccag ggcttttaca gagattatga acaaaatagg gcatgttaat tttaaactca
    24481 aaataatcga cttatgctga tcgaaaccat attgggcgtt gttaccggca tcgtaggcaa
    24541 cgtcatgaca acagttacca acgttcgcac acagaagtta aagaatgagc atgaccttaa
    24601 aatgcgcgag ttcgatatca aagagcgcga acaggaggcg cagcttcaga tagctgttga
    24661 tacagctcat tcgcaaaacg aagttgaaaa gatggaagct gaagcttatg taaaaagtat
    24721 ggaatctgcg aatgccgata ttcttaccga tgttaagctt aaagcgctat ctgataacgg
    24781 tgggaaggtc aacaaactgc tcatgttaat catgggattg gtggatgctt tcagggcgtt
    24841 catccggccg gggctaacgg cttatctggt cggactgaca accgtcatca cattccacgc
    24901 cattagcata ttgaacacga aaggcgattt tatcacggcg gtacaggccc aggagctgtt
    24961 taccaatgtg acggatatcg tgatttacct cactgtgtcg tgtgttacat ggtggtttgg
    25021 cgatcgccgg gtggctaaat tcgcctacag gcttaatgat ggcaataaac aggataaaca
    25081 gggatattaa tggcattggt caccatcata ccttcgttat cattctcgcg caacttcccg
    25141 gtgctggttg taaatgccga agaagttaca acagtagtgc tcaagcaagg caccgaaacg
    25201 gtgctcagcg aatcgtacta tcccgatgat agcggacatt tcgaaataga tcttgccgat
    25261 ttgctcacat cgctgttaac cgtcgatatt cctcagttgc ctggcgaaca ggcaggagct
    25321 ttagccgcct ttacaataac gttaaccgaa acaggtctaa ccgctgaaga gttcagtttt
    25381 accctaataa aaggcggcgt tctgaacggg gtagaggata tggatgcttt ttgcgccggc
    25441 aacatgctta cctggcatcc acagataaag cgcgtaaagc tatcggattc ggaattcatc
    25501 acgtattacg cagttgagcc agccttagta aaggcaaaag catggttcct ttcgaatcct
    25561 gttattactg ttggcgctgt gctggatgcc gggcacctgc atgtgatcaa tgctaccttt
    25621 tcggttttac gtgccatgtt cccggcatat tactatccac gatggataga tatttggata
    25681 gaaaaccaat cgggtgcagc gacctgggtg cagcgctacg aactcattga cgaatacgat
    25741 cagttcgacg atcttttcct tttcgaaaat tcgctcggcg ggctcgatac aatcaggttc
    25801 accggtcaac ttacagagca ggaaaaacat gatcaggaat cggcaaagtt cgggcttgaa
    25861 acacgggatt attacctgga ttttaaccgg gttatgagca aaaacacagg gctgtttcaa
    25921 tcacaggccg aaagcgactg ggcacgagaa tttttcgcat caacaaaccg atggtggtgg
    25981 aaagatggag tatggaaaaa gatcaccgta tcggaataca ctgttgaacg tgtgtacaat
    26041 gatatgcgtt cctataagtt tgaattttca ctcagccagc aggagccgtt aaggaatttg
    26101 gcgcgtgagg ttattccaga tccggaagat ccttacgctt tcgatttcaa tgatgatttt
    26161 aatcacgatt atctcataca gcaatgacag aagaacagtt gcaagccctg attgatcagg
    26221 agattaatga caatggtaat ggagcaatca caggaccatt acttaattca gtgctgaaat
    26281 ccattataac cgtaatattc gaccaggtag gcggagaagc ggcggtaccc gacagcagga
    26341 gcataaagca caatgccggc aagcaactgg ccgtgatgct gccaacttgg gagcctctcc
    26401 ccgggagcaa gcgcgtgaag gcaattgctt tcgatacggc gagcgaagtt gccggggggg
    26461 tatatattaa aacggacggg aaaaccgtat tcggcaacgg ggccggagag ctgacaacgg
    26521 taaattccgg cgtgttgccg gatgttacgg tagtcagcga tgacttcacc acgcagccaa
    26581 caggcgcggc gatgaataaa gcctacctgt cggttgacag caaaaacggg tacctgaagc
    26641 tggacaccgg cgccgccgtg gatacggcgt attttgtaaa gaaggacatt gcggttacca
    26701 tggacggcaa cgcgaagttt gacgtgctat tcgcccttgg tagcctgacg aatgaaaagg
    26761 ccacggtagg gctaagcacg attgatgcag accttccgga tgtggccggc atcaaattta
    26821 tttttgatcc cgccgtggac gcctactggc acctgattgc ctccgatggc ggcgaaataa
    26881 cgataaacct ggcgacgctg gtggctgctg atacgaatga gcatttgttt gaggcccggg
    26941 ttgaggatgg gaaaattacc tggtacatag acggtgcaga agttggcact acaggggccg
    27001 tgaacctgac cgatacggaa ctaaacttcg gcgtatgggt tgaaagcacg gctgccgaaa
    27061 gcaaaacggc cgtgattgac ttttacaacc tgggaggcga cagggtggat gaagaggtag
    27121 ttacctattc ggcacagcag gccgtgtcgg caccgctgaa gcatgccggg ttctggagcc
    27181 aggcaggcgc caacgcgcca accgtttcgg tactggaaaa cagccttggt accatcgcca
    27241 ttgcccgcac cgctgccgga acatatacga tcaccaaggt aggcgccttt aaggaaggta
    27301 agaccgtgcc gctcgatgac atctggaccg acgcttccgg gaacgtttac acgctggtgt
    27361 ggaccagcgc ggacgtgatg acgctgacaa cgaagaccag cgccggagcg ctggctgatg
    27421 atatcctgga taaccggttt attttcattg aaatatatca gtaatgagcg ggtttcacaa
    27481 ggctttcagc cggaacgggc aatacaagcc aggcaattac atgccggcaa ttggcgaaat
    27541 ataccagggc ggaaaagtcg ctttcatcgc tgctgacgaa tcatatatat tgatagtcgc
    27601 tgaagccgac tcggattcaa agccgtggta tccttcagcc tactcatatg taaaaggcat
    27661 cagcaacgaa tacgcctgcg gcgccgacaa tgatgcgctg atcatagctg aattcggtgc
    27721 cggcgattat gccgcgaaat tagcatccta ttcgaatata aataaatata atgactggta
    27781 tattcccagc gtcggagagt ggcaggatat ggtccagact cttacggaag gtcaatcgct
    27841 aggtttgtat aattaccaga aaggcggtat ggcaacagca tcgaacgagt actggacgtc
    27901 aaccaatatc aatacctact cgcagtatat ctatgcagta tggttcgata tgtataacat
    27961 aggctgggac gcgacaatcc caacgccgca actgaccaag cgggtgcgct tggtcaggaa
    28021 attaaccgcg ccgtttccta atccatcata aaattttcgc ctggcaggta caatttgata
    28081 aaaactgcct tacatttgtt tcttcatcga agcgcgaagc tttccaatcg caccccctgg
    28141 ggaattgaaa tattaatgat gataattaag cgcttcgaca cacaacgtta gcttttatag
    28201 ccgttgtgat ttgcttcaga tcgtgaccgc cccctaactg ggggcggttt ttttatgatt
    28261 tttcctgaaa tcggcaaaaa cacgacgaaa aaaggccctt ttttgacatg aaaaaaaaca
    28321 ctgtttgaaa atatttttct agccttagag cagcctacca tcatgcgaaa actgggcgac
    28381 tggctgtcgg cgaaccatta tgataaaata tagacgctgc cggtttgctt cccggatttt
    28441 aagccggccc gtcctgggcc ggctgtatta ttttcatttg cctgatatat ttgctgaata
    28501 tattgtaatt tgcatgagta aaccaaaaaa atcctatcat gaaacagcta attattttcg
    28561 cattattcac actattatca atcgcagcaa tatcgcagga taaggtgaca cttacaaccg
    28621 gtaaagtaat acggggtaaa ataacgctta tcaccaatac ccaaatcaat atttcttccc
    28681 catcgcgcga ttacgaatgt ccgatttatc aggttgcgtc gtatgagacg ggaggtaatg
    28741 taaagcttac cgataaatca aaactcaatc agagttcggt gatggctgag atactcggaa
    28801 acgaactaag caagacgtca cgggcttttg cggccggggc cgtattgtcg gcaggcgggt
    28861 ttatattgac taccatgccg gatcaattta ttaaaattcg atccggggac actgaaaaag
    28921 aaatttcatc aaaaacaaaa aaattgaaga atttaaaata tatatgagcc ggacttttta
    28981 ttgcaggaac aataatagag gtagcggcta ttataccgat atcgaaggcc ggcaagaagc
    29041 tcactgtttt tatgaaggac aactcggccg gggtggcgat gaagttttag gatcacattt
    29101 ccgggaagga tgatttaagt cgtttgctgg cgatagaaga gaacctatcg aggtaattct
    29161 gtgtcatatc caggctgtgg tgccggagtt gtagctgcag gtcgcgaata ttaattttac
    29221 cttccatagc taccatcccg acgctggtat gtttcaaggc ataaatgccc ctgttcttca
    29281 tttttactgc cttggcccat gctgaccatg cctcagcgat ccgggttgga gctgtgtatt
    29341 gtggcccagg aacgagcccc ttgccgcaga agtaataatc accgggaaat ttcaggttaa
    29401 gtttttccat ctcctccttg agctgatcag gaatcaccac tacttcactt tttttatttt
    29461 ttgaggttct gccgggcaga tggattacct gcgcttcaag gtcaatatca gatattttaa
    29521 gtctgcaaat ctcctgcggt ctgataaagc aataaaatac catcattgaa attatatata
    29581 agttaaaatt atactcatgg agcttctcac ttatcttctt tagttcgctg gcggaatatg
    29641 caatgataga tgtttcctca accggcaatt tctttgtcct gctaaacggg tttacatcaa
    29701 catattcgcg atatataagc tcattgaaca atgtcttcat gttcattcgc ctgtagttaa
    29761 cggtacgatt cgatagttta taatgagatt tactccagtc gaggaattcg atggcgtgac
    29821 gcgatccgaa atcattgatt gatatccttt ctagtttgtg tgctatcaga tattcccgga
    29881 acgatttgac gatatttttt aatgtaaaat atgaccgcgg cctgattgaa gcctttttaa
    29941 tctccagtat tttttccatt gccgcggatg ctgatattaa ctggatttgc tcctgttcga
    30001 aaggattcca gttttgcttt aacctcgttg aatagtactt tataagctct gatgcttttt
    30061 cgcggcgagc ggtagcagtc ttatattttg ttgaaataaa gatccggaag cgtttcattt
    30121 tccccgtttc cgggtgccgg aatgaataga aaacgaacca tcgctggttc aggtcattat
    30181 cagcatcgta gagtttcggt aatttataat tttccattgt cgctgtttgt cgcaaaaaga
    30241 aattacaaat tactataaat aacttataat taaagtattg aaaaaaaatt gtagcggggg
    30301 caggattccc gccgctattt tacctatact gataatcagt attataagtt ttaatgtaca
    30361 tatgttttta aaggctttag acctgttttg tctcaaattt tgtcgcataa aacattcatt
    30421 gaagctcgtt aaaatcagga tatagctctg tattacgatc tctgcaaaat tgctcaatct
    30481 cttgaagtca tccagtgccc tcaattactc gatgttttac ttttatcttc ttcccttcag
    30541 gctgacgcga aaggttcaat attgtttcgg cattgagacg ctgtgtggct gccatttcgg
    30601 cctgcgcctt ggccatatcc ttttgtatgg ccgaaaagtt tgatacttct gtttgcaatt
    30661 tctcgttttg cgaaacgagt gattcaataa tgcgaaattg atagtcagag ttcatgggag
    30721 taatcggagt ctctccggct gttgccggtg gtatattatt gtaataattt gatttctcag
    30781 atataaacat ctccccttca ccagtaacaa gccagtgaat attcactttt ttgaaattat
    30841 tcgaaattgc cagtaaactc tcaaaatttg gagcactttt accggttgtc catccgctta
    30901 ccgttgaagt aggtgtacct attatttcag ctagcttaac ggctttgatt ttaagctcag
    30961 ttaacaataa tatcaaccgt tcagctattg tttgcattaa tagactaatt ctaaattacg
    31021 attatttgag ctaaaatctc aattaaatgt gataatctcg tttttacgtg ttatatttgc
    31081 ttctcattag caaagataat acaaaagata cacaattcta taattaatta tgattacctc
    31141 cgaatatctc ataaaactgc gtgaatcgct accgtctcat aaccttaaaa ttctttcagt
    31201 gcggacaggt ttttctccaa cctacatttg gcaagtatta aatggtaaaa ggcaaagcca
    31261 ggatatcata gacgctgccg tcgcgctagc caaggaaaca aaagaacgac tcgaaacatc
    31321 aaaaaacgat atcgccaacc tataacctgc atttgcagaa aaaaacataa atagctatga
    31381 aacagccgct tacaaatttt ttggagttta aaggaaaaac tctgcttttt ttagcaaaaa
    31441 acgctactta ttggattgct ttaaagcctg tatgtgaagc acttggtgta aattacaaca
    31501 ggcaatttca gaatataaat aacgatctta ttctaggacc tgcatttgct gtacagcaaa
    31561 tccaggtacc tggcgatcag gtgaggaaat tcgtatgttt acctgaaagg tatatttatg
    31621 gatggatatt ctccatccag agtgcaagcc ccgaacttct ggaatataaa aaacagtgct
    31681 acgaagtgct gtatgaatat ttccacggca ccatcaccag ccggcgcgaa cttctcaagg
    31741 aaaaagcccg cctggagctt gagaggtcag caaaagaaat aaaactcctc gacaataaag
    31801 aatttattga atggcagcag cttaaagccg aagaggcccg ccttggcatt gcactcaaac
    31861 gcaacgatca ggacactgtt agcgaacaaa aagagctttt cgacagtaac ctttagcctt
    31921 aaatcaatga atttcaacat tcaacaaccg gttatgaaac gtggagccat tgagttctac
    31981 gttaataatg gcgaagcgtt tgccgaaatc gacggacaac gtatgctcct ttccgacgct
    32041 ccggtcaatg ttcacgaaat gatcaggcgc aacattgagc atcatcctgg agctatcgaa
    32101 gcccttgaat caatggggct caactctgct tcacaacagc atgaacaata tattgcctgc
    32161 atgtatggcg aatataacca tacacctgat tttctcaatt tcaggcattc ggttgatgac
    32221 gatgagttca cgcaactttt atgcggcgtg aacaactgta aattcagagg ggtactgtgc
    32281 cacaagattc atgcaaaata cagcgatcta accgacaggg aagttcaaat ctgcctccat
    32341 atccgcgccg atttctcttc aaaggaaatt gccgatatgc tggatatctc aataaatacg
    32401 gtaagcgtac acatcgccaa tatcctttca aaaataggat gcaacagccg agctggtatt
    32461 gcatcatggg catcaacaca ccttgcttaa cataaattat atggatacaa gatacaaaat
    32521 catgattgcc tcgattgcac tgatagttgt ggtaattatc attaacaata tacgttgcgg
    32581 ccgctttcgc aaaaacctga aaccgggttt attttgccgc ttttacattg acgaagattc
    32641 acgcgaaggt atcgtgcgca atattgatgg cgacacgata gtaattaccg acagttggct
    32701 caacgtttac acagtttcga tcaacgatat ttatccggca tccatctttc atttctgagc
    32761 tatgcttata cacatcgaaa gtaattcaat gctggattta ctccacacgg ccgtgcaagt
    32821 caggcttgct gccatacaaa taaagatgtc gctatcgtca actttcaatg aacaggctca
    32881 aaattcagta tctactgata tcattttact tgacgagctg ttagcaaaaa tagattattg
    32941 tataaaattt aatatcgcgt tcagaagcga gtcggaatta acggcaaaca tgctgtgcga
    33001 ttcgcttaaa actcttatac actacgacta cttagatgtt gcagctaacc atcagaaaat
    33061 caaaagctat ttcagggcag aaaaatgcaa agaactgttg aatggtatta aaagccgttt
    33121 gcaatgatat cgtacgaaat aatcaacgaa cgttacagcg ttgttgttgc gctaaaaatg
    33181 acggatgtaa atgatcacgt cagtgaattc agggagacat ttctcaatcc catcgatgca
    33241 cagaaatggg ttgccgtaaa gctcgaatac tacctgcgca aaaggatatc caattacata
    33301 attcattcta gcatgatatc gaggcattca caacatgcct actatcacac tgccgaatac
    33361 cgcaaatcga tgcaggagct gatgcggtac ctgaacgtga taaacgatca gaaactacat
    33421 cagttagcga attatgtaaa caataatgct tcatttcttg aaaaaatact gccggcacag
    33481 ggaaatccaa gctataacag cagcaaggaa atactcaggt cgatcacgaa ccttgccggt
    33541 aaattacaga aacgcgaaaa cagggtattc tgatgcgaaa aggccagtac aacgctgaat
    33601 ataaaccaaa gcccgaaaac acttccaagc gtaagaccag ggccggcatc catgtaaccg
    33661 atcacgctat tgttcgtttc cttgaaaggg tgtgcaatat tgatatcgag aacctcaagg
    33721 accagattgt aacagacgag ctgctgcgca atattgaacg attcgggcca accggagctt
    33781 atccttgcaa tcatgagaat ccgtatatgg ttgtaatcga taaaaagcag atcatcacta
    33841 ttttgccggt tagctcaaaa cattaccagg acagaactac gaaacacagg gcacatcatg
    33901 gccctggata ataacacggg atcattctcc caatgcgacc aggacaagcg gttgagtcgc
    33961 cgggcccata acccggaggt gccgggttcg attcccgggg tcgctacacc aaattacttt
    34021 aaccatggaa cttctcaatc gtaaaggctc ggtagatgat atccggtgtt cactcggatt
    34081 cataactccc aaaaccgagc gcgaaataaa gctggcgctg gcgcaggtat catcatcgca
    34141 tcacgatgaa caaatgaacc gtaagcgcac cacggttctc agcatgctta tgacgaaata
    34201 caataaactc atcaaacaac ttcaacaatg gcatcagaca gaaaagtctc actcctgcgc
    34261 ccaaacaagc taggcgaccg tatagcggta aataaagaag tacggccaaa cgataaatgc
    34321 ccgtgtggca gcggcaaaaa agcaaagtac tgctgcggat ctggcaaaag ttacatcaat
    34381 attcgcacta tatgatcact gcaggagatt ttttggcagg aggcggccga gtaaccgaga
    34441 gaacttgaag caatcctaaa ttcaaagaaa atcgctgttt aagaacaata ctatgaaaaa
    34501 agaacgcccc atcctattca gtaccgatat ggtacaggcc atcctggaag gccgcaaaac
    34561 aatgaccagg agaacaaaag gtcttgctga aatttctata aatgcaggac acacacagca
    34621 ttcttacgaa ggaaaaattc ctacggataa taatattcac gcttttgcaa gaatgtgggg
    34681 tggacatttc gcagaaacaa aacatatcaa gtgctcatat ggccagcccg gcgacattct
    34741 ttgggtgcgt gaaacgtggt gtaatgatcc aagaggtagt gaggatggta tttctccata
    34801 ttattatttc aaggcagatt tcccaaatac tgatgtatgg aaaggatcat ggaaaccctc
    34861 aatccatatg ccccgtaaag ccgcccgtat ctggctagag ataaccgaaa ttaaagctga
    34921 aaggctgtat tcgatatcaa gagaagatgc tatagccgaa ggctgcggca acgaaggcac
    34981 cgattatcct gaaggtaact ttttcgcgct ttggagaaag atcaatggca ttgattccta
    35041 tcaatccaat ccctgggtat gggtgatcac ctataaggta ctaagcacta cgggccggcc
    35101 ggaaaatctt gatttagctt agtatgcgat gccgaataat tctcaactcc attaacctgc
    35161 aactataatt gacccgtgcg ctacatcgat caggaaaaaa tatacgacgc caccgaagcc
    35221 ggcctcacca tctttcagca cttcttcccg ggtgaagacc tgcggaacaa caaaaccttt
    35281 tttaaagtaa ggccggaaga gaaatcggct tcggcacgta tatcatggta cggtaaatac
    35341 tggcgcatca ccgacttcgg caaccaggga gaaatcaacg gcatgaaagc tattgatttt
    35401 gccgtgtgga agcttgggct tccatattac gatacccttc tctacatcga acaggtgatc
    35461 atacgccagg agattggcag cggcgattat cgccggcgcc agtggacgcc tgaatacgaa
    35521 atgcgcgaaa tgagtcctga ggataaaaaa ggtgagtaca atttcacctt caaggaccat
    35581 ccaacacctg ccgacctggc cgccatcggc cgctatgttt ccgaagatat cctcgatatg
    35641 ttcaattgca ggtgcatcga aaaatatgag tactgcagca catcaaagaa gcacaaccgc
    35701 gatgtagtac acatattcaa ggcgacagag gattatccaa tattcctctt cgattatggc
    35761 gactttaaga agttataccg gccacatgac caggagaaga agaacaggtt cctgtacatc
    35821 gggaaaaagc ctaaagacta catatatgga ctgaagcaac tcgaaaaagc aaagaatgaa
    35881 tttgcttcaa ccgatgaaga tgaactgtcg ctacctgaag aaaagccaaa cgcacgggtt
    35941 attgatctgt ttcggtgcag cggcgaaagc gacgcgctca acctggcatc gcttggtttt
    36001 cacgtttatt ggctcaacag cgaaagcgcc gatttcgact ataagacttt ccgcaaatta
    36061 gacgatctgt gtgaaaacca ttaccagatc atggacctgg atccaaccgg ccaggaccag
    36121 gcgttaaaaa atgcaatgaa gcacatctcc ttgttcacga tcgagttgcc ggaatggctc
    36181 cgcgcaaaga aagattttcg cggcaatccg tgcaaggacc ttaaagactt cattaatatg
    36241 agcggcgaag atatcgacgc caccaggttt gattttctaa tcgttaaaaa ctcggctcgt
    36301 aaagtcaagt tttggacaaa aagcaaggat gataaaaccg gaaaggtgag ctataacctc
    36361 gatatggagt tcttctactt tttcctgaag gccaacgggt tttacatcat cgaatcaatc
    36421 tatcataaaa aagccggcta ttgctacgtg catatcgacg gcaaaatcgt ggagatgatc
    36481 caccctgatg acatcaagcg cattgttaag cgctttacta aggactggat taagagcaaa
    36541 aagcttatgg attcaataaa aatattgaat aagatcaaca cctccaacca gatcactgag
    36601 gctaacatcg aaagcattga tgccacaaag ctgaaattta aaaaccacga ccgtttcacc
    36661 gagtatattc atttcaaaaa cggttcgctc cgcatcacga aagagaaaat tgaaagagtt
    36721 cctcattcag aagtgcctaa ccacatactt ggtttgcttg aaatcaataa taaaaagctc
    36781 tctcatttgg tgaaccgtga catccggtta atggaaaagc caccggttga agtgaaagct
    36841 acccctgcat atcaggcgct gctcgatatg ctgcagcagg cagcaactga cgaagccaga
    36901 gaggtggtga acgtgcagat tgcgcagttc ccggatatcg acaaatacga agtggttata
    36961 cacgacgatg aattcatatt tctgcggttc ctgagagatc tttcgcgcat acactggcgc
    37021 aaggagagtg agcaaaaaat accgctaacg cccgaagaga gaaaagaaga acagctttcg
    37081 ctggccaacc tgctcttcgt tatagggtat cactgcgcgc aatataagga tcccggcaag
    37141 ccctggctca cctttctgca ggatatgaaa gtaggtgata taggtaccgc atccggccgg
    37201 tcggggaaaa gcctgttcag tcaggctatt acctacgtgc gtaccagctt ttacaagggc
    37261 ggccgcacgt tgaacgataa aagcgcctac cagttttttt atgacggtat gaccgaattt
    37321 cacgactaca tcgaggttga cgaccttcac gaatatgccg aatttggttt cttctacacc
    37381 caggtaacag gaaaacgtga agttaaccca aagaattaca cgcctttcac cctcgattat
    37441 gaggatagcg ggaagatgct tatcagctcc aactatgaac tgcagaatgt tgacagttcc
    37501 acagttggcc gcctgctcaa ctgcggcgtt agcgattact atcatgaaaa gagcaagttt
    37561 aacgattacc acgaaacccg gtcgccgctt atcaagttcg gacgccggct gtacgacgac
    37621 ttcaccgaag atgaatgggt aaagttttac aaccttattg catactgcat tcaactgcag
    37681 atgaacttct acaagataca gccgccagcc gggaatcttg aaaagcgtca attgcgccgg
    37741 gtaatgagcc agggcctcgg tcgtgatgag gaattcttcc gctgggcaaa cgactacttc
    37801 attctgtgga acgattcgga aggccggcga cctgaatttt cgcccgtgga acacggttac
    37861 ttcaacacct acatatacag gaacagccct tttgagaact tccagagccg gctgagcaaa
    37921 aagcagcaaa gtgaatatag gacaggaaaa tttaaaaagc acctcgaagc ctggtgcgaa
    37981 tattgggggt acgaacttaa cccggaagac atctgcatcg ataaggataa taggaggata
    38041 cttcgcacta tcgacgggaa aacagaagaa ctgttttaca tcagcacatc aaaactgagc
    38101 aaccaggagc agccgaaggt catcccgttg gtgccagcac aggaagatac cgataacgcc
    38161 ttgccattct agtaaagcat atgacacact atgacattga taaaattgta gcccgcaggt
    38221 tcggcataac agttcgacga cttacaacag gtaccgatcg ccgcaaggca accattgcac
    38281 gagccatggc cgtaatgctt tgctacgacg tattaaaatt atcaacctta aaacttaaga
    38341 aaatatacgg taagcaggcg cactcaacca ttattgagaa catacaaaca gccaggaacc
    38401 tggccgaaac taatcgcgga taccgtacca tcctcgacgc atgccggcac gaaattgaga
    38461 tgaaatcctc caggtcaaaa atcaggaaac aatgttacaa cttgcactat cgcattcgcg
    38521 aaaaaggcat ccgtatcgac agcaagcaaa agaccatcag cgttacagaa ggcgaaatga
    38581 taaaaatcag cgaccgtcag acgcgccggc tcatcgaccg ttatcattac tcaattcaac
    38641 tgtcattgtt atgagctgcg ggatattatg taattgcgcc acacacaccg acgctgttct
    38701 tcgcgatgcc gaatcgtttg tttgcgagcg cagtggccgt ggtatttatc gccggcgtcc
    38761 cgatggcatg cgactggcca ccatgggcga ttttgctaat ccggacggat cgttacgcat
    38821 cggcatggcc ttcctacttg ctattgagca ggcgcatact tactggtatg ttcaccgcat
    38881 aacgaaagac accgatcgcg ccgaactgca aaagttcatc gattcggaat tgtattatgt
    38941 aaaaaaaacg aaataagatg ttcagcgaac acacgaaaca catacagatg gccacgcttc
    39001 cggcaggata tgaaatagta actaccggga atatccagcc tggggacatc aggtggaatg
    39061 cctgggaaga ttgctggaat attgataatc cggtatcggc cgaaaagcat aagcttatca
    39121 ttaacgatcc ggtaagtaat tttcacggtg tttgccgtaa aagttttcag gtaacagatt
    39181 cacaacttcc tgagaatcgc gtaaaagaaa acgaaccaga cgtgaggaca gaatatgtaa
    39241 accttgaatg gttttatgaa tgcaggtaat aacggacagt tgtatgaaac gcttgctgct
    39301 ccactccacg ctccacccct taaagagtag agtagagcgt agagcgtaga gtgcgccaaa
    39361 aagctgatag caagtgtttt atacaacgtg ttataagctg ggcggtaaat acttgatgaa
    39421 cttgatacgg agaacaaaaa taaaaagccg aagggcggga aaaaattaaa ctcttaaaag
    39481 gcatgatttt gatattaaaa atattttgta tgtttgtggt gctggataat tcaattttaa
    39541 ttcatgatgc cacaaacatc gaacaaaagt ctgaaacaaa gaaaaagggt aggcgaacgg
    39601 ttgcaagtac cgcagtcttt tcaaggcatc gctttgaatt gtccagcagc gacctacccc
    39661 ctttgtttaa caatcaaaat tttaatgtca tgctggacaa aaaagacgaa atggttaccc
    39721 cgtgcgaggc atgtggacaa ccgcaagaac tcaccattga gttaatcctt aaaaacttct
    39781 ttcaaagaaa cgaggcccag gaggtgaaaa accgactttg ggaaatgttt attggtttta
    39841 ttggctctcc tgattttgac ggatggaatg ccattgaccg ttctaacctt gcgttccaat
    39901 acaaggaact atgcacatta gttgaccaac tcgaatttat tcaccttaaa caaacagcct
    39961 gattatggaa acaacagctt tagtaatgtt ttcccaaaca gtaaagtatc agggaaacga
    40021 tatttggata aaaccgtttt gtgacttctt cggaattagt tacaaatggc aggtagaagt
    40081 tctaaaaaaa gaccacattt tagcaagtat ggtcaggaaa aactgtaatg aaactttatt
    40141 tggagataaa agagaacgtg ttttattgcc aaaaaaaggg tttgtcagat ggattcaatt
    40201 aataaatcca aaaactgtac gagaagaatt acaagaaaaa ttcaaacagt ttcaggaatt
    40261 agtatttgat tacctatatg gtagtgctga agacgaagaa caaataaagg ttcattacaa
    40321 ccgtatgcaa aagctcgaaa ggctttatag caagattgga actgaaatta agagagaaaa
    40381 agcaattgta gcaacccttc ttaatggcag gtacacccaa ttaagcttaa actttaatac
    40441 ggagcaccaa ctaacagatg ggcgatagcc cttctgttta gcccggcagg gctgcgcctt
    40501 ttaagtgttt aatttttttt gcgggtgggg gctttttatt ttctttcaaa ttagcactgc
    40561 cctaaatacg aagaactgac gcagccttgc ttataacgtt tggtgtatga gctgttattc
    40621 attacctcgt aaaccttttt cttttaaaaa ttaatctaaa aatgaaaaat atttttcagc
    40681 ctgttgattt tgaatgttta ttgcatactt ttgtagtgcg agacatctta atattcaatg
    40741 agccgaaatc aaaacagact tctttcaatc cacaggggtg gcgaacggtt gcaagtaccg
    40801 aagtcgtttc aaggctcagc tttgaggtgt ctcgcggcga ccacctcttt ttctttaatt
    40861 ttaaatcatc tgttatgcga gacacagaag acgaagcaaa tgagcttatg ctcgagaatt
    40921 tcgatgccat tcgagattcc cacagccgcc accggcccga actgatcctt tcattatttg
    40981 aaagaaacaa accacaatta atcaacgatt ttctggttga aatggacgcc aaaaacaagg
    41041 cgtactattt tattattgaa tccggccact tctcggcctt tagtgagtat tgccttaacc
    41101 ctaaaaaaca aaaacacaat gcagcaaaaa tttgagtata acaatttgcc ggtgcgtact
    41161 gtaaccgatg acaacgaaca aatatggttc gctggtattg atatttgcaa tatccttgga
    41221 tatgcaaaag cctctacaac aattgaacgt ttagaagaag atgagaagaa gctggagtat
    41281 ctcacagata cctcaggtca gcaaagaaag acctggagta taaacgagtt cggtttgtat
    41341 tctttaattc taagtagcac aaagccagag gctaaagtat ttaaaagatg ggtaactcat
    41401 gaagttttac ccgctatacg aaaagctgga aagtttacca cagaagaggt aaaagaatac
    41461 gaatacagcc tacaaacatt ggcaagtgaa attgaaaaac tgaaggataa aaaggatgaa
    41521 catcagaagt gtgttaatga tttaaagaag gaaattgaat tgaaaacact cgaaatgatt
    41581 gccgtaatta agatggatag atcacaaatg aggatcgagt ttcccgaagc gtaaaatgct
    41641 tgtataccaa aatgaataat agcttatgta ccatgttata tgcaggctgc cgtatttttt
    41701 cttttctaat taccgaaagg gctttcggca gcttgcatta taacgtaaaa gcatacccgt
    41761 caggcgggga ttttaaccac tatatttcaa tagaa
//
LOCUS       DAOPYB010000009        39964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_867_length_39964_cov_10.073217, whole genome shotgun sequence.
ACCESSION   DAOPYB010000009 DAOPYB010000000
VERSION     DAOPYB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 39964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39964
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_39964_cov_10.073217"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKH58_01540"
     CDS             complement(<1..226)
                     /locus_tag="PKH58_01540"
                     /inference="COORDINATES: protein motif:HMM:NF036634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87738.1"
                     /translation="MNYTEFLETYASHPNYRAPRGNNLYAKSWQTEAPLRMLLNNLDR
                     EVAEDSENLIVYGGTGQAARNVQAVKDIIRY"
     gene            complement(371..1951)
                     /gene="hutH"
                     /locus_tag="PKH58_01545"
     CDS             complement(371..1951)
                     /gene="hutH"
                     /locus_tag="PKH58_01545"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNX87739.1"
                     /translation="MKTQKSNIFHFGEDFLTIGTALKLARNNIKGVLTPETISRVQAN
                     RDVVLKIVHKKKVVYGINTGFGPLCTSLISKEDTKKLQYNLLMSHSVGVGNFIDKEIA
                     KLMLILKLHALCKGYSGVAVETVERILWHIENDIIPCVPEQGSVGASGDLAPLSHLFL
                     PLIGMGKVWYNDEQVPMDVVLKRLDMKPVDLGPKEGLALINGTQFMAAHAILLLEKLQ
                     NCLDHADIIAAMNLEAMMGSIKPFDEELHQLRPHSGGIYVALRMRMLLSHSEIVKSHK
                     KCNRVQDPYSLRCIPQVHGASRNAWLHLKETILTEINSVTDNPIIFSEDKTISGGHFH
                     GQPIALPIDYAGLAAAELGNISDRRIYLSMMESVPGLPKLLMEHTGLNSGFMILQYST
                     AALVSENKSLCFPASADSIPTSLGQEDHVSMGSVAVRKSLRIVENLEKILGIEMMCAA
                     QGFDFRKPMKSNVLLDDMHDLIREHISFAETDRLFSEDIEKAIKLIQSKEIIKRLNET
                     AKKHHIDLKNESDKVFGI"
     gene            complement(2188..3567)
                     /locus_tag="PKH58_01550"
     CDS             complement(2188..3567)
                     /locus_tag="PKH58_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX87740.1"
                     /translation="MTAFFYGEFSYLCTRLTEFMKSNKEIYQVTYPIFLSLIAQNIIN
                     VTDTAFLGRVSEVDLGASAIGGVFYIAVYMVGFGFSQGAQILIGRRNGERNFAAIGPI
                     FNSSLIFNLMLALIIFLVSLLFIPHLMQYLVSSQHVYEATIEFLNWRIYGFFFAFLTT
                     VFRAFFVGITRTRVLTYSAVITALTNILFGYLLIFGKFGFPEWGIAGAAIASVLAELA
                     GLIYLIFATLKQKDLHIFGLFSFRKIEFETIKKILELSVFIMFQYFISISTWFMFFIF
                     IERLGERPLAVTNIGRSLYILLMIPGSALSTTINTMVSNMIGEGHKDQVLPFMKKVTG
                     ITLAIVLPMMIFTFVFPQLFARIYTDDHTLMLACVPTLRVVSVAMVFCGIGSIIFNTI
                     SGTGNTRTAFVIEFFTLFFYIGYVYYTAIILHSSVEIVWMSEFVYWIIIGSLGLWYLL
                     KGNWRKKEI"
     gene            complement(3602..4900)
                     /gene="nhaD"
                     /locus_tag="PKH58_01555"
     CDS             complement(3602..4900)
                     /gene="nhaD"
                     /locus_tag="PKH58_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNX87741.1"
                     /translation="MITLIISVFVIGYAAIAFEHNIKINKTASALITGVICWMIYILF
                     SADKHLVNEELLLHLGDISSILFFLMGAMTIVELIDSYNGFEVITEKITQTNKRSLLW
                     IIGILTFFLSAVLDNLTTTIVMVSLLRKLIKDHDTRLFYVGIVVIAANAGGAWTPIGD
                     VTTTMLWIGGQISAFNIMKGLFLPSFISLAVPLIVLSFTMKGDVKRPIVAAESYKMLS
                     RNQQLFIFILGISMLIMVPVFKTLTHLPPFMGMMLGVAIMWIVTEIMHHHHTGKDEEH
                     VKRTLVSVLTRIDLPSILFFLGILLAIAGLEVSGVLGNLAVFMDEKIGNLNSIVISIG
                     LASAVIDNVPLVAATQGMYDLAQFPMDHYLWEFLAYCAGTGGSILIIGSAAGVAAMGM
                     EKINFFWYVKKISWLALIGYLAGALYYMLEYYFSHGAFYH"
     gene            complement(5058..6845)
                     /gene="lepA"
                     /locus_tag="PKH58_01560"
     CDS             complement(5058..6845)
                     /gene="lepA"
                     /locus_tag="PKH58_01560"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNX87742.1"
                     /translation="MNKIRNFCIIAHIDHGKSTLADRLLEFTQTVSGKDLQAQVLDNM
                     DLERERGITIKSHAIQMNYTYQNETYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     ATQGIQAQTISNLYMAIEHDLEIIPVMNKMDMDNAMPEEVEDQIVELLGCKRQDVLRA
                     SGKTGMGIEEILEAIVEKIPAPKGDPDAPLQCLIFDSVFNSFRGIIAYFKITNGTIKS
                     GDLVKFVATGKEYNADEIGILRLDMSPTNTLSAGNVGYIISGIKTSKEVKVGDTITHV
                     KRPCDKAIAGFEEVKPMVFAGVYPIETEDFEDLRSSLEKLQLNDASLTFEPESSVALG
                     FGFRCGFLGLLHMEIVQERLDREFDMNVITTVPNVSYKVHTKKGDLLEVHNPAGLPDI
                     MAIETIDEPYIRASVITRTDYIGQIMTLCLGKRGDLIKQEYVSGNRIELVYDLPLGEI
                     VFDFYDKLKSISKGYASFDYHMNGYRPSKLVKLDILLNGEPVDALSSLIHFDNATSLG
                     RRMCEKLSELIPRQQFDIAVQAAIGAKIIARETIKAVRKDVTAKCYGGDVTRKRKLLE
                     KQKKGKKRMKQIGSVEVPQKAFLAVLKLD"
     gene            complement(7091..7264)
                     /locus_tag="PKH58_01565"
     CDS             complement(7091..7264)
                     /locus_tag="PKH58_01565"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNX87743.1"
                     /translation="MNPDFYKALEISGIGIAGVFIFMTLFYFVIIGLDKAFPNEKKPK
                     KAPQAVDENSGDE"
     gene            complement(7278..8429)
                     /locus_tag="PKH58_01570"
     CDS             complement(7278..8429)
                     /locus_tag="PKH58_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNX87744.1"
                     /translation="MGVEQLFPAIAGMTWQNLVMIIVGFTLIFLAIKKQYEPTLLLPM
                     GFGAILVNIPFTAALTQIDPATGAVVEGALDVFFNAGIATEVFPLLIFVAIGAMIDFS
                     PLFQRPSLLLFGAAAQFGIFVTMVLATIFGFNIKEAASIGIIGAADGPTSIIVSTILA
                     PNLLGPISVAAYSYMALVPIIQPPVVKLLTSKHERKMRMHTDANKKVSRTVLILFPIL
                     VTVLAGLIAPSSLALIGFLMFGNLIRECGVLNGLSKTAQNELANLVTILLGITIASTM
                     TGARFVTVDTLKILGLGIFAFVFDTAGGVLFAKAMNLFLSEEKKINPMIGACGISAFP
                     MSARVIHGMGLKEDPHNYLLMPAISANVGGQIGSVIVGGLILLLVPLFA"
     gene            complement(8561..9484)
                     /locus_tag="PKH58_01575"
     CDS             complement(8561..9484)
                     /locus_tag="PKH58_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNX87745.1"
                     /translation="MTKSGLKTDSGEKTTKSLSPVVDWIVRLVKGTIVGIGAILPGLS
                     GGVLAVIFNLYDPLLRFLADLRKNFVKNVLFFIPVGIGVALGIFLFAIVVEKAFGKYA
                     AEFTCLFIGFVIGTFPSLYRTAGKSGRNKKDIIILIISALIIFSLMLIGEQELVNVTP
                     SIPVWFGSGALIGLGMIVPGMSPSNFLIYFGLYDKMATGIKNLDMAVVIPLTIGVIAC
                     VLLFAKLASYLFRKHYSGMYHFILGMVVGSSLAIFPVIVFPAFRADQLAVAGLSFVGA
                     AVFCVILLLIGAVASYFFSKVEERYPREDIF"
     gene            complement(9511..10575)
                     /locus_tag="PKH58_01580"
     CDS             complement(9511..10575)
                     /locus_tag="PKH58_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNX87746.1"
                     /translation="MGTLTMWWVAFIVIFIIVYAIDLAVTARRKETESISTALKWTGV
                     WISIALLFAVAIYFFFTQNPDSKMNTAHVMGTKFLSGYLTEYSLSVDNLFVFIMIFSM
                     MGINDKNQPKLLMIGIMISIVLRILFILVGMELVERFEWIIYIFGAILLYTAYKMVFS
                     KDEDNVDPKSNFLYKGASKLFPVDPDAHNPKFFARIDGKRFITNSFLCLLVIGSTDIL
                     FAIDSIPAIIGVIKEGATNILTASEENFLAISSNVFAVMGLISLFFALRGIMGMFRFL
                     KVGVSFILFFIGCKMLLGAIPAVAAFFSAHSWVSLAVIIFTLAFSIVLSMLISENKEN
                     EELKVELKVEKLKEERHDEI"
     gene            10906..14217
                     /locus_tag="PKH58_01585"
     CDS             10906..14217
                     /locus_tag="PKH58_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNX87747.1"
                     /translation="MSKQGNILVKGARVHNLKNIDVEIPRNKLVVITGLSGSGKSSLA
                     FDTLYAEGQRRYVESLSAYARQFLGRMSKPEVDYIKGIPPAIAIEQKVNTRNPRSTVG
                     TSTEIYEYIKLLYARIGKTYSPISGELVKKHEADDVVEVAMEYPDGTKLMVLATVTPK
                     EGISREERLNLFLQNGFSRIVIDGETVRIDELLSKKTIPDTYREIFLLIDRLVVDHSP
                     SGISRLTDSIETAFLEGKDACTLEIITDTETKRVNFSKAFEADGIQFELPSEHMFSFN
                     NPLGACPVCEGFGKVIGIDEDLVIPNKSLSVYEDAIVCWRGEVMSEYKKQLIRCADKF
                     DFPIHKPYIELSNEQRELIWTGNKYFEGLNSFFQMLEANQYKIQYRVMLARYRGKTIC
                     PACKGTRLKQEAAYVKINQVPITELVLMPVTRLNDFFENLELDEHDASVSKRLLIEIR
                     NRIHFLLDVGLGYLTLNRLSNTLSGGESQRINLVTSLGSSLVGSLYILDEPSIGLHSR
                     DTHLLIKVLHQLRDIGNTVVVVEHDEEIMRAADYIIDIGPGAGRAGGNVVWQGKPPIQ
                     PPKSPKSPIPLRDFPLKGKDAHIDLSALNKNLQEQDESDNKVYKTANPLNYELLKENA
                     DQRKKNPTDAENFLWNNLRGKNLENFKFRRKYVIGDYIVDFVNLSSQLVIEVDGGYHK
                     DEKVRQQDEIRTAYLNEKGFSVLRFTNEEVLNNIEKTLETILIALRQSPPLKGGLGRG
                     SDLGLSYTLEYLLGLETIPLPLHRRIWNNYIEVLGARENNLKGINVKFPLNVMTVVTG
                     VSGSGKSSLVKSVFYTALKKYYGGVAEKTGQFGSLRGSMHLAKDIEFVDQNPIGRSTR
                     SNPVTYIKAYDEIRKLFADQQLSKQMGYQASHFSFNTPGGRCEECQGEGTVTVEMQFM
                     ADLVLECEHCHGKRFNQDILDVTYRGKNIYEVLDMTVNQAIEFFSEHTGNTEKRIVKR
                     LQPLQNVGIGYVKLGQSSSTLSGGESQRVKLASFLANEKSEPTIFVFDEPTTGLHFHD
                     IKTLLKAFDALITKGHTVIIIEHNMEVIKCADHVIDIGPEGGEKGGNIVFEGTPEDLV
                     NCECSYTGKFLKDKL"
     gene            14277..15476
                     /locus_tag="PKH58_01590"
     CDS             14277..15476
                     /locus_tag="PKH58_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87748.1"
                     /translation="MKRFFLSLGALCLILVVNAQDIAKTINDYEETKSTTISKGRKLL
                     LDQFVSDRIDKVEEVYVYLNDKIADADYAAFSAFEQINLSCWTRRYEYALKEILYLDS
                     INTARKEGKTATVTRIYPANDMLGTRIYEKTAPVLPSVLQNIETSNLSREEKDALQLI
                     MRDIYLTGDDHRRSLYQDSINALSTKFLSDYPASPYSSYIRNAVRFVYHRNDWGFGYD
                     FGLGYTQVFGSMVDIYKNGFNANGGFDIFYKQTVAYIRFLISTSGTKHAVVANHGNVI
                     PAETFMNFNHFEFSLGYALPELKRLTVTPFAGIGAFGVAYGAEQDRNDNTILKNAGKT
                     VLCYQGGLNMDFDLFNKNVSGGKMPVRLRCTYIRPSNSAYSYIPSGIFTVSLGFAIQG
                     WSTKREL"
     gene            complement(16014..18842)
                     /locus_tag="PKH58_01595"
     CDS             complement(16014..18842)
                     /locus_tag="PKH58_01595"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNX87749.1"
                     /translation="MKINSFKFILSALLTVMSAILFAQQSQLQPLPMDPKIRYGKLDN
                     GLTYYIRANKEPKERAEFHIAQNVGAILENDDQNGLAHFLEHMAFNGTKNFPGKGIIN
                     YFEKNGVKFGYDINAYTSLDETVYRLSNVPTMREGLLDSALLVMHDWSNFILLQDDEI
                     DSERGVIREEWRTGNNAFRRMWKQSNAKKYPGSQYAKRDIIGDTAVINNFTYKTLREY
                     YKRWYRPDQQAIVVVGDIDVDKMEAKIKQMFSDISKKDNFGERPVYEITNNKEPIISI
                     VTDPEASKTQIELEYKKDKLPREVKLAMPGYAMGLVNSLISSVLSERFEEIAMQADAP
                     FAGAYGAYGELVKSKDAFQMAVVPKEGKELEGLKALLLEAEKMKRFGFTNAEMERAKT
                     NMLKNFEKSYNERDNQKSMALAEEYIRNFLADEPIPGIEFEYQTAQMLLPQLKTEMIN
                     QLAKSYVSDTNLVVSVTAPEKAGVKVPTKEQILTALDDTRKAELTAKAEEVLNKPLLD
                     KAPKAGKVKKIKQNPAMETTEMILSNGVKLIFKPTTFKKDEISFTAFSDGGISKVHNV
                     ADLPSASMADGIVQSNGIGNFSAVELSKVLTGKIASVSPYIDQYEEGMNGNSSVKDFE
                     TMLQLLYLYFTQPRKDDNSFKAIMNMYRTQLANVASNPNKAFSDSIQVTTSAKDPRTV
                     LQNLQTLEKVDQDKALEIYKQRFANPADFTFIFVGNIDPNNKETQQLLATYLGGLKTD
                     KKNTEKFDKVYREFPKGKVNNYFTFKMKTKKASNRIYYTADIPFSINNQVAVSAIGDI
                     LDIRYLESIREKEGGSYGVGVYGGMANVPKDQASILMQFDTDPEKQAKLMGIIHDEIK
                     QIIDNGPLKDDLDKVKENLMKQYKQDIEQNNWWRGKLKAYYQDGLNYRTDYKAAVDAL
                     TSESIQKTLKSIVDQQNVIEIVMKPAAE"
     gene            19371..19850
                     /locus_tag="PKH58_01600"
     CDS             19371..19850
                     /locus_tag="PKH58_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNX87750.1"
                     /translation="MAEELKINGAAGKEARYRELIPQLQALTEGESNLTANLANIAAA
                     LKEVFGWWWVGFYLVKENELVLGPFQGPVACTRIALGRGVCGTAWKERRSILVPDVNK
                     FTGHIACSSASVSEIVVPIFNQQQEVAGVLDVDSELYDVLDETDVKFLEEIAGIINI"
     gene            complement(20195..21601)
                     /gene="sufD"
                     /locus_tag="PKH58_01605"
     CDS             complement(20195..21601)
                     /gene="sufD"
                     /locus_tag="PKH58_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNX87751.1"
                     /translation="MNNTDTNNRVKSTSTSSVKQDGAEFQYIELYSQHHETIKKHSAY
                     VLNEVRDEAFKKFCTLGFPDSRLENYKYTDLSESLTVDYGLNINRVRFAVNPYDVFKC
                     DVPGISSYLYFVVNEGFYPVSTPRNETLPQGVIIGSLKTAAEEHPELVKKYFGKLSVK
                     QNDGLVAFNGAFAQDGFFMYVPENVTIDKPVQLINILRSNADFMANSHNLVILEKGAK
                     AKLLVCDHTVDDVRFFSNRVTEVFVGENAQYEHYKLENVHNKTTNLCTLLVDQDTSSS
                     FLGNVITLHNGLTRNTVEIDLDGEHCETLMCGMALSDKNQQIDNFTSVIHNKPNSHSS
                     ELFKYVLDDSAKGGFTGKLYVAKDAQKTQAYQTNKNILLKGTAKMRTKPQLEIYADDV
                     KCSHGATIGQLDDKAMFYLRQRGITEKEARLLLMYAFTADVIDNIRIDALQDRIKLLV
                     EKRLRGELSKCEGCVICK"
     gene            complement(21653..22390)
                     /gene="sufC"
                     /locus_tag="PKH58_01610"
     CDS             complement(21653..22390)
                     /gene="sufC"
                     /locus_tag="PKH58_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNX87752.1"
                     /translation="MLEIKNLHANVNGKEILKGLNLTVKAGEIHAIMGPNGAGKSTLA
                     SVLTGNPAFEVTEGKVIFEGKDLLELNPEERASEGIFLSFQYPVEIPGVSMVNFMRAA
                     INEQRKYRGQEPIAATDFLRMMREKKELVEMDNKLTNRSVNEGFSGGEKKRNEIFQMA
                     MLDPKLSILDETDSGLDIDALRIVANGVNKLKTPENASIVITHYQRLLDYIIPDFVHV
                     LYDGKIVKTGDKYLALELEEKGYDWLK"
     gene            complement(22540..23976)
                     /gene="sufB"
                     /locus_tag="PKH58_01615"
     CDS             complement(22540..23976)
                     /gene="sufB"
                     /locus_tag="PKH58_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNX87753.1"
                     /translation="MSDDILHEITQSDYKYGFSTDIETEVIPVGLNEDVIRLISAKKK
                     EPEWLLDFRLKAYRHWLTMQMPTWAHLKIPEIDYQKISYFAAPRKNAPKSLDEVDPEL
                     LKTFDKLGIPLDEQKALSGMAVDAVMDSISVKTTFRENLAEMGIIFCSFSEAVEHHPD
                     LIQKYMGSVVPYTDNFFATLNSAVFSDGSFVYIPKGVRCPMELSTYFRINAMNTGQFE
                     RTLIVADEDSYVSYLEGCTAPMRDENQLHAAIVEIVVMKNAEVKYSTVQNWYPGDKDG
                     KGGIYNFVTKRGICKGENSKLSWTQVETGSAITWKYPSCILLGDNSTAEFYSVAVTNH
                     HQQADTGTKMMHIGKNTRSHILSKGISAGFSNNSYRGLVRVNPKADNARNFSQCDSLL
                     LGDKCGAHTFPYMDVNNDTAIVEHEATTSKISEDQIFYCNQRGIDTETAVGLIVNGYA
                     KEVINKLPMEFAVEAQKLLQITLEGSVG"
     gene            24239..25078
                     /gene="nfo"
                     /locus_tag="PKH58_01620"
     CDS             24239..25078
                     /gene="nfo"
                     /locus_tag="PKH58_01620"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNX87754.1"
                     /translation="MKYIGAHVSASGGVENAPLNARNIGAKAFALFTKNQKQWQTSPL
                     TAKSIELFKQRCQEFSFSPSQILPHDSYLINLGHPETEGLEKSRNAFLDEMQRCEQLG
                     LDRLNFHPGSHLNTISQDDCLARIAESINLTLDKTTGVTAVIENTAGQGTNMGHTFEQ
                     IAQIIDQVKDKSRVGVCIDTAHSLAAGYELRTLEGFTETFKRFDTIIGFNYLKGMHIN
                     DSKKDLGSRVDRHDNLGKGLMGLDTFRYIINDSRFDNIPLILETPDETLWTEEIALLY
                     SFD"
     gene            complement(25154..29146)
                     /locus_tag="PKH58_01625"
     CDS             complement(25154..29146)
                     /locus_tag="PKH58_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNX87755.1"
                     /translation="MRKYLTLLLLVLVFTIQSVAQVSKTISVTSAGSLTSLLTATEKT
                     TVTDLTITGNLDARDFKCLRDELSVLSVLDLTNVSIVAYSGNDGTFPTSWGFAEYPDN
                     TLPQYAFNKDVTIGKLTLSKVILPSSINAIGNFAFQYCSNLTDVNLPVSLTTIGNNAF
                     YACTNLNNFIIPDNVTSIGASAYQYCHAITSITFGSKLNEIGNQAFYECHSLKIIDSK
                     NTVPPVLLGDPFGFTYLSMVYVPAQSINTYRSTNGWKLLNIGSSTLITINNASAGSLK
                     SAFTSQTQVPLSSITKLKITGQLNNSDIYLIRDSLKILMEIDLSESTFSANTLPSGAF
                     QGKGIIVDVKLPESVMVIGDNAFNSCVNLNSHVPLPSGLTTIGTGAFYNCKLMTGDLI
                     LPSNVQTIGNNAFDGCRSLAGSIVLPSNVVSIGNSAFKDCVGLTGSLVLPESVVTLGS
                     YAFQNCTGLNGNLTLSGNLSKIPNGAFATCTGLTGSLTIPASVTSIEGSAFTECSKIT
                     ELTLPKNLTSIGDEAFYSCSGLTKIISLNSLPPTIYNNTFRFVNKSACQLKVPIGSKS
                     AYQAADYWKQFSLISEDAGTFNIIVNSGTNGKVKVNSTIIESGTAFLADKNSSVKFTI
                     VPDDYYVVDTLRYNSVDVTSQIVNNQYITPIVVGNSVLDVKFHTAQKITIKIDPQSGG
                     GTIMYNGSILKNDTVLAVYKGLTKTFEIVPAENYALDSLLFGGTICSLPLTNNLYTTD
                     SIKTDAVLKAIFKKITYKITLQTNAGGMVKENGAELTNGTMLVVEQNAHKTFTIIPQE
                     GYVVDSIIYAGNDVKSQLTNNQYTTPAINSNTNLKVVFKESTEVFTVRILKGLNGTIK
                     ENNLLLNNNDLVSAKINAVKLFTIIPDEGYQLDSVRFNGVDVKSQIVNNVFSAGPLNS
                     NDTLKVTFKKILQVYNITIQIGSGGGVKENNIALSNNTIIQAQEGTSRTFSILPGSGY
                     EVATLTYNANDVKSQISNGQFTTPSVTGNAILNVTFAKINYSITINSGTGGVVKDGLT
                     VLSNNASVNVEAYSTKTFTIQPDAGYEVATLTLGGLDMKNQLVNNQFTTPNIIANTTL
                     NVTFKLQSTSNTYKLKVVFGVGGIIKENNNVLKSDTLLTTNNGSTRTFTIIPDVGYRI
                     AAVIYGGTFVTTQVINGMYVTPLITSDKTLAVTFEKLKYNLSIKSAINGVVNLVCYHG
                     DTPTFSIAPEAGWKINSIIYNGSDITTEASNSEYTVPPVTKNTTLTISYESLTASPVI
                     GISDVKVYTNNSNIIIDGLSDDEMVNVFTMNGSRICSLKSQGELVQIPVRQNAIYLVK
                     TGGKTYKVIL"
     gene            complement(29756..33139)
                     /locus_tag="PKH58_01630"
     CDS             complement(29756..33139)
                     /locus_tag="PKH58_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87756.1"
                     /translation="MKKVFQIKLKSITRVFAMVMALSLVTFTGCKSYDQDIDKLNTDL
                     TSLKTELTALNTATQNALQLQITTLNTDITALKARVTTLEQTGATDAEVAAAIAAAKT
                     EIMSKVVTLEAFNAYKATVDADLASLKTKVDAAATKAELTALATRLDGKILEIENTLT
                     ALGAKVTTLQTSVNLLGTQLDEAKARISKNEADIAKNITDLAALKADVQARLSILEGV
                     LSIKNGKSEVIDKIQLDLTAQKALIDANTAKIADLQAQLDALKARTVALETAVAKNTA
                     DIAKNTADIAKNTADITAIRTELALINDNLKTVANAMHLNFAVLSSRLSSLVYAGDLS
                     GTELSRFYVNGIEAINFSSLMSKCDPITPAITIAYHLNPSFIKEADIETGNMAFVVRK
                     GSNAITNDYYLPVQGANDRAVQVAAIYDHIDATTGIIYVKVSVNDYNTLIGEEGGNYV
                     GGSLETFRSIALQIPLSQKAVQENAIQLDNSGKMVVVGSTEYPANRVITSDYVRLYGE
                     NMYAQENVYLARNNAPTYSILPLTLADAKLLSVKDVDNATTTAPQVVTLPYGKTLDLI
                     NEVKAIFSHAPFDIVKYGLNYKFDLLDENGNTIVYNRGSNNTDQQQFIKINDAAKGTI
                     EAKVFTQAEIAAAQGRTPIVRVWMYNAKNPSCPVLLGFVKIYMAEKPLPTPITVPFTF
                     ADMEAKCADVAGRTTVQQMNEKLYNAVGLSKEEFHAVYTLVPVPGAVSEIKNPDGQTT
                     DTWLLEWNLTAQQIWSALMTANPATFTQTVEYVPSLPTVYPKITITFTRKFTKPADLN
                     IAEGSLIKKYWYDMNGANNATVFDEVKHNVNVPDVNESTTTNCTIKNDLSQAFLQNAD
                     KSLKGFSNYEYYFVPNVVGQPAISQPVITANGVNYQFTPSTDGKQLLNGSEVIATINP
                     FVANVGDILELNRNSTTAKLLLNYDKEVLRARIGIRTKYCQELNFLQPITVNGKPTFD
                     VVFVRPIKAAALSPDHFVDGKSFGQTNTYIPITKMVSLSDWRDYSFASYPNLYGYYGV
                     TNIALSTTKSIETNLNGTRVALSNFPSLQVAYAASVPGVSPAAQYGYLTYQNNGNVIG
                     NSFELYVPVTITYYWGEIVNTWVTVTVDKTVGPSGIRRK"
     gene            34924..35916
                     /locus_tag="PKH58_01635"
     CDS             34924..35916
                     /locus_tag="PKH58_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNX87757.1"
                     /translation="MSKSGLSFKYLITILTGVLISCCNTESPHLTINFDKRASMPGNG
                     RSSAVGFAVNGKGYVTLGRNADNVQLNDCWEYDPQLNTWKQKSSFPGIPRVKASAIVL
                     NNKAYIGLGHNNAVGVYNPTSCLKDFWEYKPETDTWRRLADFPSNFTDACISFVYNYA
                     IYIGIGFSESSYGSELWKYIPAEDKWIRLNDFTGRQRFGAVACQNDRHIFFGTGYRTG
                     NLNDWWEYHPESDSWTELESMPDNGRVNSVSLCIDNRIFVATGRYFGGNLTGGHVKSD
                     LMEYGLSIDGWHLRGEIPDGNRENAIAFVIDGRGYIGFGENDNGVLNDFWCFKP"
     gene            36011..37369
                     /locus_tag="PKH58_01640"
     CDS             36011..37369
                     /locus_tag="PKH58_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNX87758.1"
                     /translation="MAVFSQKDSICFSTEITGVYSTGKYAPFWLQSNRYGRISSSPAS
                     TFLSTGIEKAYNKPQRLFDYGFKADLLTRTDKTKTELFPHELFVKARLAVFNLTVGSR
                     EQIFGNQDSTLSSGGLLFSKNTRPMPEIGAGIENFTPVPLTLGYVELKGGLRHGWFTD
                     NLYTREILLHHKFAYIRLGGKLPVHVSYGIEHVAQWGGTDPVYGPLPGGLKNYARIFL
                     AKSGGEDASVSDQINVLGNHIISQGLKLELNVTGIRLNGYWQNISEDRPIRFIGSNRI
                     NKPDGLWGISIKNSRFPYIRGVLYELLNTTDQSGPYHDKDGLIYGGTDGYFGGSYPTG
                     WSYYSRTIGTPFITSPLYNENGTLHTTNSRVRLHHFGMEGNIRGVEYRVLASFSRNYG
                     TYGSPVNMTNKSFLLEVQKYISLLSGFELSCSAGSDAGNLFGNSVGVLFSIRKNGLLL
                     KY"
     gene            complement(37446..38645)
                     /gene="argG"
                     /locus_tag="PKH58_01645"
     CDS             complement(37446..38645)
                     /gene="argG"
                     /locus_tag="PKH58_01645"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNX87759.1"
                     /translation="MKEKVVLAFSGGLDTSFCAKYLAEEKGYEVYTAVANTGGFNEQD
                     LQVIEEKAYRLGAVKHVSLDITQEYYEKSIKYMVFGNVLRNGTYPISVSSERIFQAIA
                     IINYAKEIGADYVAHGSTGAGNDQVRFDLTFQILAPEIKILTPTRDMTLTREYEINYL
                     KQHGYVADFTKMEYSINKGLWGTSIGGKETLKSEQTLPESAYPSQVVKQTEETLTLDF
                     EQGEIAAVNGVKNTNKVELITQVEEIANKYGIGRDMHVGDTIIGIKGRVGFEAAGPMV
                     IINAHKMLEKHTLTKWQQYWKDQLGNWYGMFLHEAQYLEPVMRDIEQFLQSSQQNVTG
                     RVIVKLRPYCYTLVGVESDFDLMKTDFGEYGEVNKAWTADDVKGFTKILGNQMKIFYN
                     VQKRNEK"
     gene            complement(38650..39324)
                     /locus_tag="PKH58_01650"
     CDS             complement(38650..39324)
                     /locus_tag="PKH58_01650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX87760.1"
                     /translation="MNKLNTENNDIIVQLADTSHLPYVQTILTTIEEAAKIRGTGIAK
                     RSPEYIQQKIKESKAIIALKGEEFAGFCYIETWGNKKFVANSGLIVVDKFRGHGLAKR
                     IKQKAFQISRERYPDAKIFGLTTGLAVMKINSELGYRPVTFSELTNDEAFWKGCQSCV
                     NYDILQRTGRKHCLCTGMLYDPAWQENNGSEKKTRKNKMWKLIQVMGEWMSPKILLQK
                     QQKEKE"
     gene            complement(39344..39814)
                     /locus_tag="PKH58_01655"
     CDS             complement(39344..39814)
                     /locus_tag="PKH58_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HNX87761.1"
                     /translation="MKNKRERLSVISEMLRLQVVGSQEELLELLREKGYDVTQATLSR
                     DLKVLKVAKTPLSNGAYKYMLPPQIKPIPGELNAHNFTSHGAVLNLEFSGQLAVIKTK
                     PGYASAIAWDIDNRATDEVLGTIAGDDTILLIPREGVARDEITKIINLIFHEKN"
BASE COUNT        11954 a         8250 c         7679 g        12081 t
ORIGIN      
        1 aataacggat aatgtctttt actgcctgca catttcgtgc cgcttgtcct gtaccaccgt
       61 aaacaatcag gttttcggaa tcttcggcca cttcgcggtc gaggttgttg agcagcatgc
      121 gcaaaggtgc ttccgtctgc cacgattttg cgtacagatt gtttccgcgg ggtgcccgat
      181 aattcggatg agaagcataa gtttcaagaa attcggtgta gttcataata tctgccccct
      241 aaccccctaa agggggaatt ataaatcgca gagggaatgc gaaatttatg tttaacaata
      301 tcgatgtttt attttcacta ctaaatcaaa atatcttggt ttattccccc tttagggggt
      361 tagggggcaa ctatatccca aacactttat cactctcatt tttcaaatca atatggtgtt
      421 tctttgctgt ttcatttagc cgtttgatga tttcttttga ttggataagc ttgattgctt
      481 tttcaatatc ttcagaaaat aaacggtctg tctcagcaaa tgaaatatgt tcacggatta
      541 agtcatgcat atcatcaagc agaacattag atttcattgg cttgcgaaaa tcaaatccct
      601 gagcggcaca catcatttct atgcccagaa ttttttccag attttctaca atccggagac
      661 ttttgcgcac tgccacagaa cccatgctca cgtgatcttc ctgaccaagc gatgtgggaa
      721 tgctgtcggc gcttgccgga aaacaaagcg atttattttc gctcacaagg gcggctgtgc
      781 tgtattgcaa aatcataaaa ccggaattta agcccgtgtg ttccatcagc aactttggca
      841 agccgggcac actttccatc atcgaaagat agatacggcg gtcggatata ttgcccagtt
      901 cggcagctgc aagtccggca taatcaatcg gcaaagcaat gggctgtccg tgaaaatgcc
      961 cgccactgat ggttttatct tcgctgaaaa taatcggatt gtcggtaacg gaattgattt
     1021 cggtgagaat ggtttctttc agatgcagcc aggcattgcg cgaagcgccg tgaacctgcg
     1081 gaatacagcg taatgaatac ggatcctgta cgcggttgca ttttttgtgt gatttcacaa
     1141 tctcggagtg agagagcaac atccgcatac gcaaagctac ataaattccg cccgaatgcg
     1201 gacgaagctg atgtaattct tcgtcaaaag gcttaatcga tcccatcatc gcctctaaat
     1261 tcatggcagc gataatatca gcatgatcca ggcagttttg cagtttttca agcaacagaa
     1321 tggcatgagc cgccatgaac tgtgtgccgt ttatcaatgc caaaccttct ttcggcccca
     1381 aatccaccgg tttcatgtca agcctcttca atacaacatc cataggcact tgttcatcgt
     1441 tgtaccacac ttttcccatt ccaattaaag gaaggaataa atgtgaaagc ggagccagat
     1501 cgcccgaagc gcccacagaa ccctgttccg gaacgcaggg aataatatca ttttcaatat
     1561 gccacaaaat acgctccacc gtttccacag ctacacccga atagcccttg caaagcgcgt
     1621 gaagcttcaa aataagcatc agtttggcaa tttccttatc gataaaattg cccactccca
     1681 cgctgtggct catcagcaga ttgtattgca gctttttggt gtcttccttg cttatcagcg
     1741 aagtacaaag tggtccgaaa ccggtattga tgccgtacac cacttttttc ttatggacaa
     1801 ttttgagtac cacatcacga ttggcctgta cacgggatat ggtttcagga gtgagaacgc
     1861 ctttaatatt gtttcgggca agctttaaag ccgtgccaat ggtaagaaaa tcttcgccga
     1921 agtgaaagat attggatttt tgtgttttca taagtaaatg atctgccccc taacccattt
     1981 taacgggaat ttttgagtgt ctgttaaaag aattgcacta taacaaatct agtttttgcg
     2041 aatataaatt cgattttaat gcaaatataa tgaaaaggtt tgttgaaatg ctggaaacaa
     2101 ctcaaaataa aaaaagatta tttttggaat tagcaaagtt atgtttaatc tgtatatttc
     2161 cgcattcaga gggttttgga tggcagttta tatctccttt ttccgccagt tccctttcag
     2221 tagataccat aatcccaggc tgcctatgat tatccagtaa acaaattccg acatccagac
     2281 aatttcaaca gaagagtgaa gtatgatagc agtataatat acatatccta tgtagaagaa
     2341 cagcgtgaaa aattcaatca caaaggctgt acgggtgttg ccggttcctg aaatagtatt
     2401 aaaaataata ctgcctatcc cacaaaacac cattgcaact gaaacaaccc gtaaagttgg
     2461 gacacaggcc agcattaatg tatgatcatc ggtatatata cgggcgaaca actgaggaaa
     2521 tacaaaggtg aatatcatca tcgggagtac aatggcaagt gtaattcccg ttaccttttt
     2581 cataaatggc agtacctgat ctttatgccc ttcaccaatc atattgctca ccatggtgtt
     2641 gatggttgtg gataatgccg aaccgggtat catcaggagt atatacaaac ttcgaccgat
     2701 attggttacc gccagcggac gttcgcccag gcgttcaata aaaataaaga acatgaacca
     2761 ggtggaaatg gaaataaagt attggaacat gataaaaacc gatagttcca aaatcttttt
     2821 aatggtttcg aattcaatct tccggaaaga aaacaaccca aatatatgca aatctttttg
     2881 tttcagggta gcaaaaatca aataaatcag acctgccagt tcagccaaaa cagaagcgat
     2941 agccgctcct gcaattcccc attcgggaaa tccaaattta ccaaaaatca gcaaatatcc
     3001 gaataatatg ttcgtcaacg cagtaattac agctgaatag gtcaacacgc gggttcgtgt
     3061 aattcccaca aaaaatgccc tgaaaactgt tgtgagaaaa gcaaagaaaa atccataaat
     3121 acgccagttc agaaattcaa tagtggcttc atacacatgt tgcgagctga ccagatattg
     3181 cattaaatga ggaataaaca gtaacgatac aagaaaaatt atcagtgcca gcattaaatt
     3241 aaaaatcagg cttgaattga atatcggtcc gatagccgca aagtttcttt cgccgttcct
     3301 gcgaccaata agtatctgag cgccctgact aaacccaaaa ccaaccatat acacagcgat
     3361 ataaaatacg ccgccgattg ctgacgcgcc caaatccacc tcacttacac ggccaagaaa
     3421 ggctgtatct gttacattga taatgttctg cgctatcagc gaaaggaaaa tcggatacgt
     3481 tacctgatat atttctttgt tggatttcat aaattctgtg agacgggtac aaaggtatga
     3541 aaattcacca taaaaaaagg ctgtcattgt tatcgacagc cttgattttt atattgtttc
     3601 atcagtgata aaatgcaccg tgtgaaaaat aatattccag catgtaatat aaagctcctg
     3661 ccagatatcc gataagtgcc agccagctga tttttttcac ataccagaag aaattaattt
     3721 tttccatacc catggcagcc acaccggcag cagaaccaat tatcagtatg ctacctcccg
     3781 tacctgcaca gtaagcaagg aactcccaca aataatgatc catcgggaac tgtgctaaat
     3841 catacatgcc ctgtgtcgct gctaccagcg gcacattgtc aatcaccgct gaagccaatc
     3901 cgatagagat tacaatggaa ttaagatttc cgattttttc atccataaac acagccagat
     3961 tacccagaac acccgatact tccagtccgg ctattgccag caaaatgccc aggaagaaaa
     4021 gaatacttgg taaatcaatt cgtgtcaata cacttacgag ggttcttttt acatgttctt
     4081 catccttacc agtgtggtga tggtgcataa tttcagtaac aatccacatt attgccacgc
     4141 cgagcatcat acccataaaa ggaggcaaat gagtgagtgt tttgaaaacc gggaccatta
     4201 tcaacatgga aattcccaga ataaaaataa ataactgttg attgcgcgac agcattttat
     4261 atgattcggc agctactatc ggtcgcttca catcaccctt catagtgaat gacaacacga
     4321 ttaacggcac tgccagactg ataaaactcg gaaggaaaag tcctttcatg atgttgaatg
     4381 cggaaatctg acctccaatc caaagcattg tggtagtcac gtcaccaatg ggtgtccatg
     4441 ctccaccggc attggctgca atcactacaa tgccaacata aaacaaccgg gtatcatggt
     4501 ctttgatcaa tttgcgaagg agcgatacca tcacgatggt cgtcgtaaga ttatcaagca
     4561 cagcagacag aaagaaggtg agaatgccga ttatccagag cagcgatcgt ttgttggtct
     4621 gtgtgatttt ttcggtaatc acttcgaaac cattataact gtcgatgagt tcaacaatcg
     4681 tcatggctcc catcagaaaa aacagaatac ttgatatatc tcccaaatga agtaataatt
     4741 cttcgtttac aagatgttta tcggcactga acaggatata aatcatccag catattacac
     4801 cggttattaa tgccgaagct gttttattga ttttgatgtt atgttcaaaa gcaattgcag
     4861 cataaccgat tacaaataca gaaataatta gtgttatcat aaatttcaaa aaaatgttct
     4921 taatctcccc gaaggaagtt aagaacatta cttatttgtt aattacataa atttgttttt
     4981 cataattctc aaacacaaaa tctgaatttt agttcaaaaa ttataataat tttccacttt
     5041 tattttgttt gggtccctta atcaagcttc agcacggcaa gaaatgcttt ttgaggtacc
     5101 tctaccgagc ctatttgttt cattcgtttt ttaccttttt tctgtttttc aagcaatttt
     5161 cgttttcgtg tgacgtcgcc gccataacat ttggctgtaa catctttacg taccgcttta
     5221 attgtttcgc gtgcaataat tttagcaccg atagccgcct gaacagcaat atcaaactgt
     5281 tgacggggta ttagttcgct cagcttttca cacatccgtc ttccgagtga ggtcgcatta
     5341 tcaaaatgta tcagcgatga caaggcatca accggctctc catttaatag gatatcgagt
     5401 ttgacaagct ttgaggggcg ataaccattc atgtgataat caaatgaagc gtaacctttg
     5461 gagatgcttt tcagtttatc ataaaagtcg aacacgattt cacccagcgg taaatcatat
     5521 accaactcta tgcgattgcc tgatacatac tcctgtttaa tcaaatcgcc gcgcttaccc
     5581 agacacaacg tcataatctg accaatataa tctgtgcgtg taattaccga agctcttatg
     5641 tagggttcat caatcgtttc aatagccata atatcaggca aaccggcagg attgtgcact
     5701 tcgagcaaat ctcctttttt ggtatgcact ttatacgaca cattcggaac cgtagtgatt
     5761 acattcatat cgaattcacg atccagtcgt tcctgcacga tttccatatg aagcagtcca
     5821 agaaatccgc aacggaaacc aaagcccaat gccactgagg attcgggttc aaatgtaagc
     5881 gaggcgtcgt tgagctgtaa tttttccaga gaagaacgca aatcttcaaa atcttccgtt
     5941 tcgataggat acaccccggc gaaaaccatc ggtttcactt cctcaaaacc tgcaatagct
     6001 ttatcacaag ggcgttttac atgtgtaatt gtatcaccca ccttaacctc tttcgaggtt
     6061 ttgatacctg aaataatgta tcccacgtta ccggcactca gcgtattggt tggcgacata
     6121 tccaacctga ggataccgat ttcgtctgca ttgtattcct taccggttgc tacaaatttc
     6181 accagatcac ctgatttgat ggttccgttg gttattttga agtaagcgat tatcccccgg
     6241 aaagaattga aaacagaatc gaagatgaga cactgaagcg gcgcatccgg gtcacctttg
     6301 ggtgccggaa ttttttcaac aattgcttcc agtatttctt cgatacccat ccctgttttt
     6361 ccgctggcac ggagtacatc ctgacgttta cagcccagca attccacaat ctgatcttcc
     6421 acttcttcgg gcatggcatt atccatgtcc attttattca tcaccgggat tatttccagg
     6481 tcatgttcaa tggccatata caggttcgaa atggtctgtg cctgaatacc ctgggtggca
     6541 tccactatca gcaatgcgcc ttcacaagca gcgattgaac gtgatacttc gtaagagaaa
     6601 tccacgtgtc ccggagtatc tatcaggtta agtgtgtaag tttcgttctg ataggtataa
     6661 ttcatctgga tagcgtggct ttttatcgta atgccacgtt ccctttcaag atccatattg
     6721 tccagcactt gcgcttgcag gtccttaccg gatacagtct gggtaaattc cagcaaacgg
     6781 tcggcaaggg tgctttttcc atgatcaata tgggcaataa tacaaaagtt gcggatctta
     6841 ttcatctgtt atatctgtct tttacttata agggtacaaa agtacaaaaa aaactcatct
     6901 ctttaattgt tgtattatca ggatgaataa aattttcgtt ataagtcgtt gaattactga
     6961 aaaaaaacaa caaacaacta taaattcatt tcaattaagt acgaaataat cagtttttca
     7021 aaataaaaaa gccggaaaat tatctgttac aatgataatt ccggcttttt attaaatcct
     7081 gaatgctgat ttattcatca cctgaatttt catcaacagc ttgtggcgct tttttgggtt
     7141 tcttctcatt cggaaaagct ttgtcaagtc cgatgattac gaagtaaaac agggtcataa
     7201 atatgaatac accggcaata ccaataccgg aaatttctaa ggctttataa aaatctgggt
     7261 tcatagtttt gtgaatttta agcaaatagt ggaactaata ataaaattaa accgcctacg
     7321 ataacggaac caatctgtcc gcctacgtta gcactaatag cgggcatcag caggtagttg
     7381 tgcggatctt ccttcagacc cataccatga ataaccctgg ccgacatggg gaatgcagag
     7441 ataccgcaag ctccgatcat cggattgatt ttcttctctt cggagaggaa taagttcatt
     7501 gctttggcaa ataatacacc gcctgcagta tcaaatacaa aggcaaaaat acctaatccc
     7561 agaattttga gggtatctac agttacaaag cgtgcaccgg tcatggtgct ggcaatggtt
     7621 atacctaata aaatagtcac caggttggcg agttcattct gagcagtttt cgataaacca
     7681 tttagcacac cacattcgcg aatgaggtta ccaaacatca ggaagccgat aagagccagc
     7741 gatgaaggtg caataagacc tgcaagtacg gttacgagta tcgggaataa aatcaatact
     7801 gttcttgata ctttcttatt ggcatcagta tgcattcgca tctttctttc atgctttgag
     7861 gttaaaagtt ttaccaccgg cggttgaatg ataggcacca acgccatgta cgaataagct
     7921 gcaactgaaa ttggtcctaa caggtttggt gcaagaattg tggaaactat gatggatgtg
     7981 ggtccgtcgg cagctccgat aataccgatt gaagctgctt ctttgatatt aaatccgaaa
     8041 atggtagcta aaaccatggt aacaaagata ccaaactgag ctgcagcccc gaaaagcaac
     8101 aacgacggac gctggaacag cggcgaaaaa tcaatcatcg ctccaatggc cacaaaaata
     8161 agcaacggaa agacttcggt tgcgatacct gcattgaaaa acacatccaa cgcaccttca
     8221 acaaccgctc cggttgccgg atcaatctgt gtaagcgctg ctgtaaacgg gatatttacc
     8281 agaatggcac caaatcccat aggcaacagc agcgttggtt catactgctt tttaatggct
     8341 aaaaaaatca aagtaaaccc gacaataatc atcaccagat tttgccaggt cattcctgca
     8401 atggcaggaa atagttgttc tactcccata ataaataatg ttttttattt tttgtattaa
     8461 attcaataaa ttaattatca agattatatg caaaataatt aaacgaaaat caaaaaaagg
     8521 acttcggata atcatatact tgcttaagtt ctccaatgtg ctaaaaaata tcttctcttg
     8581 gatatctttc ttcaactttg ctgaaaaaat aggacgcaac cgcaccgatc agtaaaagaa
     8641 tgacacagaa aacggcagca cctacaaaac tcaatcccgc gacagctaac tggtctgccc
     8701 taaatgccgg gaaaacaatg accggaaaga ttgccagtga cgagcctaca accattccga
     8761 gaataaagtg atacataccc gaatagtgct tcctgaataa ataggaagct aattttgcaa
     8821 agagaagcac acatgctatt actccgatgg taagcggtat taccaccgcc atatccaggt
     8881 tttttattcc ggtagccatc ttgtcgtaaa gcccgaaata aatcaggaaa ttcgaaggac
     8941 tcatacccgg cacaatcatt ccaagaccaa tcaatgcgcc ggagccaaac caaaccggta
     9001 tgctcggggt tacatttaca agctcctgtt caccgatcag catcagggag aaaatgatta
     9061 acgctgaaat gataaggatg ataatatcct ttttattacg accagatttt ccggccgttc
     9121 tgtaaagtga tggaaaagtg ccgattacaa agcctataaa cagacaagta aactcagcgg
     9181 catattttcc gaatgctttt tcgaccacga tggcaaaaag aaaaataccc aacgccactc
     9241 ctattccaac aggaataaag aaaagcacat tttttacaaa attctttctt agatcggcca
     9301 gaaaccggag taagggatca taaaggttaa atatcaccgc caatactcct cccgacaatc
     9361 ccggaagaat tgctccaata cctacaattg tgcctttaac aagacgaact atccagtcaa
     9421 cgacaggact caggctcttg gttgtttttt cgccggaatc cgtttttaat ccactttttg
     9481 tcatcagttt tttattattt catttcatta ttaaatctca tcatgtcttt cttctttcag
     9541 cttctcaact ttcagttcta ctttcagttc ttcgttttcc ttgttttccg aaatgagcat
     9601 ggagagaaca atagaaaatg ccagtgtaaa aattattact gccagagata cccatgaatg
     9661 cgcagagaaa aatgcggcaa cagccggaat ggcaccaagc aacattttac agccgatgaa
     9721 aaacaggata aaacttacac ctactttcag gaaacggaac attcccataa tccctctcag
     9781 tgcaaagaac aaggaaatca aacccataac tgcaaataca ttagaactga ttgccagaaa
     9841 gttttcttca cttgctgtaa gtatattggt tgccccttcc ttaattaccc ctataatagc
     9901 cggaattgag tctattgcaa ataaaatatc ggtagaacct attaccagta aacaaagaaa
     9961 actgtttgtg ataaaccttt taccatcaat acgggcaaag aactttggat tatgcgcatc
    10021 cggatcaaca gggaaaagct tcgatgcacc tttgtacaaa aagtttgatt ttggatcaac
    10081 attatcttca tctttcgaaa ataccatttt ataagctgta tagagcagaa tggctccgaa
    10141 tatataaata atccattcga atctttcgac caactccatg ccgacaagga taaacagaat
    10201 acgcaaaacg atagaaatca taatcccgat catcagcagt tttggttgat ttttatcatt
    10261 aatacccatc atggagaaaa tcatgataaa cacaaaaagg ttgtcgacag agagtgaata
    10321 ctctgtcaga taccctgata gaaattttgt acccataaca tgggctgtat tcatcttact
    10381 gtccggattt tgtgtaaaaa agaaataaat tgcaaccgca aaaagcaacg cgatgctgat
    10441 ccagactccg gtccatttta aagccgttga aatactttca gtttctttgc gtcgtgcagt
    10501 cacggccaaa tcaatagcat aaacaatgat gaagattact ataaatgcaa cccaccacat
    10561 agtaagtgtt cccataaaat taaatttgtt atttgattaa ttcaatatat ttcatctgaa
    10621 aaatatccgt aaagttattg ttatcaattc cacagaacaa atcattgcac tgataagcaa
    10681 atacttacac actgatacct gttttcaaaa atctgaatgc taagatattg aatattttta
    10741 tcattttaat gatattgttt atgattcttt tcaaaaagaa caaatcataa aactactgta
    10801 aaaactgttt tataattaaa aagaagtctg caggccattc gttgattttt ataaaattac
    10861 gtacctttgc aaattgcatt tttctcaaaa ttcataaacc gaagcatgtc aaaacaagga
    10921 aatatactgg taaaaggtgc ccgggttcac aacctgaaga atattgatgt tgaaattccc
    10981 cgtaacaaac tggtggttat caccggatta tccggatcgg gaaaatcctc tctggctttt
    11041 gacacgcttt atgccgaagg tcaacgccgg tatgtggaaa gtttgtcggc ttatgcacgt
    11101 cagtttctgg ggcggatgtc aaaacccgag gtggattata ttaaaggcat tccaccggcc
    11161 attgccatcg agcaaaaggt caacacccgc aatccccgct caacggtggg aacttccacg
    11221 gaaatctatg aatatatcaa gttgctttat gcccgtattg gcaaaaccta ttcaccgata
    11281 tcaggcgaac tcgtcaaaaa acatgaagcg gatgatgttg ttgaggttgc aatggaatac
    11341 cccgacggca caaaactgat ggtgctggct actgtcacac ccaaagaagg cattagtcgt
    11401 gaagaacggc tgaacctgtt tctgcaaaat ggtttcagca ggattgtcat cgacggggag
    11461 acagttcgta ttgatgaact tttatcaaaa aaaacaatac cggacacata tcgggaaatt
    11521 ttcctgctca ttgaccgcct cgtagtggac cacagccctt caggtatcag caggttgaca
    11581 gattcgatcg aaacagcttt tcttgaagga aaagatgcct gtacgctgga gatcataact
    11641 gataccgaaa caaagagagt aaatttctcg aaggcattcg aagccgacgg cattcagttt
    11701 gaactgcctt ccgaacacat gttcagcttc aacaatccgc tgggcgcatg ccctgtatgc
    11761 gaaggtttcg gtaaagtaat cggcattgac gaagacctgg taattcccaa caaatcactc
    11821 tcggtgtatg aagatgcgat agtttgctgg cgtggcgagg taatgagcga atataaaaaa
    11881 caacttatcc gctgtgccga caagtttgat tttcccattc acaaacctta tattgaactg
    11941 agcaacgaac agcgtgaact tatctggacc ggcaacaaat acttcgaagg attgaattct
    12001 ttcttccaga tgctggaagc aaaccagtac aaaatacaat accgggtgat gctggcacgt
    12061 tatcgtggaa aaaccatttg tccggcctgc aagggtacga gactgaaaca ggaagctgcc
    12121 tatgtcaaaa tcaatcaggt tcccatcaca gaacttgttc tgatgcctgt aacccgactg
    12181 aatgatttct ttgaaaatct cgaactggac gaacatgacg cttcggtgtc gaaacgattg
    12241 ctgattgaaa tcagaaaccg catccatttc ctgcttgacg tgggactggg ttatctcact
    12301 ctcaaccgct tatccaacac cctttcgggt ggtgaaagtc agcgtataaa cctcgtcaca
    12361 tcactcggca gtagcttggt cggttcactt tacatactcg atgaaccttc cattggactg
    12421 cattccagag atacacacct gctcatcaaa gtactgcatc agttgaggga cattggaaat
    12481 acagtggttg tcgttgagca cgatgaagaa atcatgcgtg ctgccgatta cattattgat
    12541 atcggtccgg gcgccggaag agctggtgga aatgtggtgt ggcaggggaa gccgccaatt
    12601 cagcccccta aatcccccaa aagccccatt ccccttcggg acttccccct caaggggaag
    12661 gatgcgcaca tagacctttc agcattaaat aagaatttac aggaacaaga tgagtcggat
    12721 aataaagttt ataaaacagc caatccgttg aattatgaat tattaaagga aaatgctgat
    12781 cagagaaaga aaaatcccac cgatgctgaa aattttcttt ggaataatct aagaggaaaa
    12841 aatttagaga atttcaaatt tagaagaaag tatgtaatcg gtgattatat cgttgatttc
    12901 gtaaatcttt ccagtcaact ggtaatcgaa gttgacggag gatatcataa ggatgaaaaa
    12961 gtaagacaac aggacgaaat acgtacggct tatctcaatg aaaaaggttt cagcgtctta
    13021 agatttacaa acgaagaggt tctgaacaac atagaaaaaa cgctcgaaac tatcctcatt
    13081 gcactgcggc aaagccctcc cttgaaggga gggttgggga ggggctctga cttagggctt
    13141 tcctacactc tcgagtatct cctcggtctg gaaaccattc cgcttcctct tcaccgtcgt
    13201 atatggaaca attacatcga ggtgctggga gcgcgcgaaa acaacctgaa aggtattaat
    13261 gtaaagttcc cgctgaacgt catgacggta gtgacgggtg tgagcggttc ggggaaatcg
    13321 tcgctggtaa agagtgtatt ttataccgcc ctgaaaaaat attacggcgg agtagccgag
    13381 aaaaccggac agttcggttc gctcaggggc agcatgcacc tcgcgaagga tatcgaattt
    13441 gtggatcaga atccgatagg acgctccacc cgttcaaatc cggttaccta catcaaagcc
    13501 tatgacgaaa tccggaaact ctttgccgac cagcaactgt ccaaacaaat gggatatcag
    13561 gcatcacatt tctcattcaa cacacccggc gggcgctgcg aggaatgtca gggcgaaggt
    13621 actgttaccg tagaaatgca gtttatggcc gatttggtac tggaatgtga acattgccac
    13681 ggcaaacggt ttaatcagga cattctggac gttacctaca ggggaaaaaa catctacgaa
    13741 gtactggata tgaccgtcaa ccaggctatt gaatttttct cagaacatac cggaaacact
    13801 gaaaaacgta tcgtgaaaag gttgcaacca ttacaaaacg tgggcattgg gtacgttaaa
    13861 ctgggacaat cgtcgagtac cctgtcgggc ggtgaaagcc agcgggtgaa acttgcctcg
    13921 tttctggcca atgagaaatc agaaccaact attttcgtat tcgacgagcc taccacaggt
    13981 ctccattttc acgatattaa gacactgctg aaagcatttg acgcgctgat aacaaagggg
    14041 cacacagtca ttatcatcga gcacaacatg gaggtaatta aatgtgccga tcacgtcatc
    14101 gacatcggac ctgaaggagg tgaaaaagga ggcaatattg tttttgaagg tacacccgaa
    14161 gatctggtaa attgcgaatg ttcctatacc ggaaaatttt tgaaagataa attataatca
    14221 ttcaacctgt ttggaaaaat aaatttttca ttacttttgt aaaaacacaa aattatatga
    14281 aacgattttt tctgagtctt ggagctttat gtcttattct tgttgtaaat gctcaggaca
    14341 tagccaagac aattaatgat tacgaagaaa ccaaatctac aaccatttca aaagggcgga
    14401 aattactgct cgatcagttt gtaagcgacc gcatcgacaa ggtcgaagaa gtttatgttt
    14461 atctgaacga caaaattgcc gatgccgatt atgcagcttt cagcgcgttc gaacaaataa
    14521 acctgtcgtg ctggacacgc cgctatgaat atgctctaaa agaaatattg tatcttgatt
    14581 caataaatac agcacgcaaa gaaggaaaaa ccgcaaccgt aacccgaatc tatcctgcaa
    14641 atgacatgtt gggtacacgg atttacgaaa aaacagcgcc cgttctgcct tctgtccttc
    14701 agaacatcga aacgtccaac ctcagccgcg aggaaaaaga tgcgctgcag ctgattatga
    14761 gggatattta tcttaccggt gacgatcacc gcaggtcttt gtatcaggat tccatcaatg
    14821 cgttaagcac caaattcctt tccgattacc cggcatcccc ttattccagt tacatccgta
    14881 acgcggttcg gtttgtttat caccggaatg actggggctt tggatatgat tttggtctgg
    14941 gatacacaca ggtttttggt tcaatggttg atatatataa aaacggattt aatgccaacg
    15001 gcggatttga tatcttctat aaacaaactg tggcatatat tcgctttctg atcagtacgt
    15061 cgggcacaaa acacgccgtt gtggcaaacc atggcaacgt aatcccagct gaaacattta
    15121 tgaacttcaa tcactttgaa ttcagtcttg gatatgcact gcctgagttg aagcgactga
    15181 ctgttacccc gtttgccggt ataggcgctt tcggagttgc ttacggagca gaacaagacc
    15241 gcaacgataa cacgatatta aagaatgccg gaaaaacagt gctttgttat cagggtggat
    15301 taaatatgga ctttgatttg tttaataaaa atgtttcagg cggcaaaatg ccagtcagac
    15361 tgcgttgcac ttacatacga ccctcaaact cagcttactc ctacataccc tccggtatct
    15421 ttaccgtttc tctgggtttt gccattcagg gctggagtac caaaagagaa ttatagtagc
    15481 atgacggccc cccagccccc taaaggggta gtaagagaaa gaatgattac gtcacaacaa
    15541 gtgacgccct cgctaattta aaaataaggt acagatccta tactaattct aatgctatta
    15601 aggttgaaat atacaaaata aggtctttaa ttgcatctaa ggaccttatc tccttgaatt
    15661 aaacttcgta gtaatttgga taaccggaat taaaacctta ttaccttatt aatttaacgt
    15721 gagttcgaca taagaaatag tccgttagga cttaaatatc aatagaaagc gtgtttttgt
    15781 attggttcat ttcccgtagg gaattgatat atttatcccc taacggggaa tttaatttat
    15841 ttaataacct atttctatgg atattaattc cctacgggaa tacagacaat ttaattgttt
    15901 tggttaaatg aaatcgaact cacgttaact aaaacaaatc ctcagggaaa ttaaaaaaca
    15961 acagggaaaa ttcagtttga attttccctg ttgtttttta tatccggata atgttattcc
    16021 gcggcaggtt tcatcacaat ttcaatcaca ttttgctgat caacaattga tttcagggtt
    16081 ttctgaatgg actctgaagt caaagcatca acagcagctt tgtaatcagt acggtaattc
    16141 agtccatcct gatagtaagc tttcagttta cctctccacc agttgttttg ttctatgtcc
    16201 tgtttgtact gtttcatcag attttccttt accttgtcga ggtcgtcttt gagcggacca
    16261 ttgtcgatta tctgtttgat ctcatcgtga atgatgccca tcagtttcgc ctgtttttcg
    16321 ggatcggtat cgaactgcat caggatggaa gcctgatctt ttggtacatt agccatacca
    16381 ccatatactc ctactccgta cgatccgcct tctttctcgc ggatgctctc caaatagcgg
    16441 atatcaagaa tatcgccgat agctgaaacc gcaacctgat tgttgatact gaaaggaata
    16501 tcggccgtat aatagatacg gttcgaagcc tttttggttt tcatcttgaa agtgaaatag
    16561 ttatttacct ttcctttggg aaattcgcga taaaccttat cgaatttttc agtattcttc
    16621 ttatctgttt tcagaccgcc aagataagtt gccagcagtt gctgagtttc tttgttgttg
    16681 gggtcgatgt ttcccacaaa aataaaggta aaatcagccg gattggcaaa acgttgcttg
    16741 tatatttcca acgccttatc ctgatctact ttttcgagag tttgcaggtt ttgcagcacg
    16801 gttctcggat ctttggccga agtggttacc tgtatactat cggagaaagc cttgttggga
    16861 ttactggcca cattagccaa ctgtgtacga tacatgttca tgatggcctt gaaggagttg
    16921 tcgtccttgc ggggttgcgt aaagtacaga taaagcagtt ggagcattgt ttcgaagtcc
    16981 ttcaccgatg aatttccgtt cattccctct tcgtactggt cgatatatgg cgataccgac
    17041 gctatttttc cggtaagcac cttgctgagc tcgacagcgg agaaattacc gataccgttc
    17101 gactgaacaa ttccgtcagc catggatgcc gaaggcaaat cagctacgtt gtgaactttg
    17161 gatataccac catcgctgaa agcggtgaaa gaaatttcat cctttttgaa cgtggtaggt
    17221 ttgaagataa gtttcacacc gttgctaagt atcatctcag tggtttccat cgccgggttt
    17281 tgtttgattt tctttacttt tccggctttg ggagctttat ccaatagcgg tttattcagc
    17341 acctcttcgg ctttggctgt cagttcggct ttgcgggtat catcgagggc tgtaagtatc
    17401 tgctcctttg tcggaacttt tactccagcc ttttcagggg ctgttaccga caccaccagg
    17461 ttggtatccg aaacgtacga tttggccagc tgattgatca tttctgtttt cagttgtggc
    17521 agcagcatct gagcagtctg atattcgaat tctattcccg gtatgggttc gtcggccagg
    17581 aaattacgga tgtattcctc tgccaacgcc atgctcttct gattatcgcg ttcgttatac
    17641 gatttctcga agttcttcag catgttggtt tttgctcttt ccatttcggc attggtgaaa
    17701 ccgaagcgtt tcatcttttc ggcttcgagc aacaatgctt tcaagccctc cagttctttt
    17761 ccttccttgg gaacaacagc catctggaac gcgtctttcg attttaccag ttcaccataa
    17821 gcgccgtaag ccccggcaaa aggagcatcg gcctgcatgg ctatttcctc gaaacgttcg
    17881 ctgagaacag atgagatcaa tgaattaaca agtcccatcg cataacccgg cattgccagt
    17941 ttcacttcgc gtggcagttt gtctttttta tattcgagtt ctatctgcgt tttggaagct
    18001 tcgggatctg ttacaattga gattatcggt tccttgttgt ttgtaatttc ataaacggga
    18061 cgctctccga agttatcctt tttcgaaata tcggagaaca tctgtttgat tttggcttcc
    18121 atcttatcca catcaatatc accaacaaca acaatagcct gctgatcggg acgataccac
    18181 cttttgtaat attcgcgcag tgttttatag gtgaagttgt tgataacagc cgtatcgccg
    18241 ataatatctc tttttgcata ctgcgacccg ggatattttt ttgcgttgga ttgtttccac
    18301 attcttctga acgcattgtt accggtacgc cattcttcac ggattacgcc acgttcgctg
    18361 tcaatttcat catcctgaag caaaatgaaa ttcgaccaat cgtgcatcac gagtaacgca
    18421 ctgtccagca gaccttcgcg catggtggga acgttggaca agcggtacac cgtttcgtcg
    18481 agcgaggtgt aagcgttgat gtcataaccg aactttactc cgtttttttc aaaataattt
    18541 attatgcctt ttcccggaaa attctttgtt ccgttgaaag ccatgtgttc caggaagtga
    18601 gcaagtccgt tctgatcgtc attctccaga atagcgccta cgttttgagc aatgtgaaat
    18661 tcggcccttt ctttgggttc tttattggca cgaatgtaat aggtcagacc gttatccagt
    18721 ttcccgtaac gaattttcgg gtccattggc agcggttgca gttgcgactg ttgcgcaaac
    18781 aggattgccg acataacagt cagcaaagca gacaagataa atttaaacga attgattttc
    18841 atgttgagta aaatattata taaattgatt ggactaataa tgctgtagat tacaggcatt
    18901 tcaactaccg acagacagtt ggagagttga ataaatacaa aggaaacaga attttactta
    18961 atcaccaatt attaatcaac ttttaattgt taaattaaca aaatgagtta gtttgatgct
    19021 tgagtttgca agttagaaat ttctctgttt ctttttaata gttctccagt ccccttaggg
    19081 agaatctggt ttcggtactg aactgtgttg tcatgaaata ggtttacgat aaagtaaaca
    19141 catttcatga ttaaagatgc taaggtaact ctgaggggga atttagagaa ggttaaataa
    19201 cagatagtaa tcaatcaaac aattacacgt ttaaattttc atcctgactt tctgatatgt
    19261 tcatattttg ttcactcaca acccgtaata ttatccgctc aaacaaagaa caaccgggat
    19321 aaattccggt aaaatattta cctttgtata tgtaaaacaa acaaatacct atggctgaag
    19381 aactgaaaat aaatggtgct gccggtaaag aagcacgtta ccgtgaactt attccccaac
    19441 tacaggcact gacggaaggt gaaagtaatc tgacggcaaa cctggcaaac atcgcggctg
    19501 ccctgaaaga agtattcgga tggtggtggg ttggttttta tctagtgaaa gaaaatgaac
    19561 tggtgctcgg tccgtttcag ggcccggttg cctgcacccg gatcgcgtta gggagaggtg
    19621 tttgcggcac agcctggaaa gaaagacgct ccattctcgt acccgatgtc aataagttta
    19681 cggggcatat cgcgtgcagc agcgcatcag tatccgaaat tgttgttccg atattcaatc
    19741 agcagcagga agtagcggga gttctggatg tggacagcga actatacgat gtgctggacg
    19801 aaacagatgt gaaatttctg gaagaaattg caggaataat aaatatttaa agaggccccc
    19861 taattatctc ccttaggtcg ctgaccgttg gttccccctt gggggacttt ggctgcatcg
    19921 gttttattag aatatgtaaa tttatttcag gacaaggcaa atcattcttt ctcttactct
    19981 cccttcaggg ggcttgttcc tctgcccttt ctgtcagtcc ttctgtttaa ctaccgttta
    20041 aatgtaattt ctcaaataat gaggtttgct gccccaagcc cctaaagggg atgtgaagac
    20101 cattcgggta aataaaagca cattagatat gatgaccaga ctttcattaa acgctcaata
    20161 actccccctt ttggggggct ggggggcttt ctttctattt acaaatcacg caaccttcac
    20221 atttgctgag ttctccgcgc aatcgttttt ccaccagcag ttttattctg tcctgcaggg
    20281 cgtcgatccg gatattatca ataacatcgg ctgtgaaggc atacatcagc aacaagcggg
    20341 cttccttttc ggtaattccc cgctgacgca agtagaacat ggctttatcg tcaagctgtc
    20401 cgatggtagc tccgtgcgaa catttcacat cgtcggcata gatctcgagc tgaggtttgg
    20461 tgcgcatttt agctgtacct ttcaacagga tatttttatt tgtctgatat gcctgtgttt
    20521 tctgcgcgtc ttttgctaca taaagtttac cggtaaatcc gcctttagca gaatcatcca
    20581 gtacatattt aaagagttca gagctatgac tattgggttt gttgtgaatg accgaggtaa
    20641 aattatctat ctgctgattt ttgtctgaca gcgccattcc acacattagt gtttcgcaat
    20701 gctctccatc cagatcaatt tccaccgtat tgcgggttaa accgttatga agtgtaatta
    20761 catttcccag aaaagaggag gaggtgtcct gatcaacaag cagggtgcac agattggtgg
    20821 ttttgttatg tacattctcc agtttgtaat gttcatactg agcattttcg ccaacaaaaa
    20881 cctccgttac cctgttcgaa aaaaagcgca catcatctac ggtatggtca cataccagaa
    20941 gtttagcctt tgcacctttt tcaagaatga cgaggttgtg actattggcc ataaaatcag
    21001 cattggaacg caggatattg attaactgta ccggtttgtc gatcgtaaca ttttcgggta
    21061 catacataaa gaaaccatcc tgagcaaaag ctccattgaa agcaaccaac ccatcatttt
    21121 gcttaacgga cagtttacca aaatacttct taacgagttc cggatgctct tcggctgccg
    21181 ttttcaggct tccgatgata acgccttggg gtaaggtctc attgcggggt gtactgaccg
    21241 gataaaaccc ttcgttgacc acgaaataca aatacgacga aatacccggt acatcacatt
    21301 tgaacacatc gtatggattg actgcaaacc tgacacggtt gatattcagc ccgtaatcaa
    21361 cggtgagcga ttcgctgagg tctgtgtatt tatagttttc gagacgggaa tcaggaaatc
    21421 ccagagtgca gaatttctta aacgcctcat cacgtacttc attcagtaca taggctgaat
    21481 gtttcttaat cgtttcatga tgttgcgaat aaagttcaat atactgaaat tcagctccat
    21541 cctgtttcac cgaagatgtt gaagttgatt tcactctgtt atttgtatct gtattattca
    21601 taatgacttg taaatttatt aaagttcccc gccgggggat catctggagg ctttatttca
    21661 gccagtcgta gcctttttct tcgagttcca gcgccagata cttatctccg gttttaacta
    21721 ttttcccgtc ataaagcaca tgtacaaaat cgggaataat gtaatcgagc agtctctgat
    21781 agtgtgtgat cacaatactg gcattttccg gtgttttcag tttattgact ccgtttgcca
    21841 cgatacgcaa ggcatcaata tccagcccgc tgtcggtttc gtccagaatg gacagctttg
    21901 gatcaagcat ggccatctga aaaatttcgt tccgtttctt ctccccaccc gaaaagcctt
    21961 cgttcaccga acggttggtc agtttgttgt ccatttccac cagttctttt ttctcacgca
    22021 tcatacgcag aaaatcggtt gcagcaatgg gttcctgtcc gcgatacttc cgctgttcgt
    22081 tgatggcagc ccgcataaag ttaaccatac ttactccggg aatttccacc ggatactgga
    22141 aactcagaaa gattccttcg ctggcacgtt cctccgggtt gagttcaagc aggtctttac
    22201 cctcaaatat tacttttcct tccgtcactt cgaaagcggg atttccggta agcaccgatg
    22261 caagagttga ttttcctgca ccgttcggtc ccataatggc atgtatttca cctgctttga
    22321 ctgttaggtt gagacctttt agtatttctt tgccattaac gttggcgtgt aagtttttaa
    22381 tttctaacat atacagaccc cctgacccac attgggcatt tcctgaaata tcgggggctc
    22441 aggaaattaa taattatttc gttttaatat attcttattt gtcttgaaaa tctaatttgc
    22501 atttcttacc cctcattttg gggttagggg gccgtgcttc taccccacgc ttccttccag
    22561 ggttatctgt aacagtttct gtgcttcaac cgcaaactcc atcggaagtt tattgataac
    22621 ttccttggca taaccgttta caatcagacc aactgctgtt tcggtatcga ttcctcgctg
    22681 attacagtag aatatctgat cctcgctgat tttggaggtg gttgcttcgt gttcaacaat
    22741 cgctgtatcg ttgtttacat ccatatacgg gaaggtatgc gctccgcatt tgtcgccaag
    22801 caagaggctg tcgcactgtg aaaagttgcg agcgttgtcg gcttttggat ttactcgcac
    22861 caagccccgg taactgttat tactgaaacc ggcggaaatt ccctttgaaa ggatgtgact
    22921 tctcgtattc ttacctatat gcatcatttt tgttcccgta tcggcctgtt gatggtgatt
    22981 agtaacggct acggaataaa attcagccgt tgaattatca cccagcaaga tacaagaagg
    23041 atatttccag gttatcgccg aaccggtttc aacctgtgtc cacgacagtt tagaattctc
    23101 gcctttgcaa atcccccgtt ttgtaacaaa gttatagatg ccgccctttc cgtccttatc
    23161 gcccggatac cagttctgta cggttgaata tttcacttcg gcatttttca tcaccacaat
    23221 ttcaacaatg gcagcgtgaa gttgattctc atcgcgcata ggagccgtac aaccctccag
    23281 ataagatacg tagctgtctt catcggcaac aatgagcgtt cgctcaaact gaccggtatt
    23341 cattgcgttg atacggaagt aggtggaaag ttccatcggg caacgaacac ctttgggtat
    23401 atatacgaac gaaccgtcac tgaataccgc gctgttaagc gtggcaaaaa aattgtcggt
    23461 ataaggtact acagaaccca tgtatttctg tatcagatcg ggatgatgct caacggcttc
    23521 gctgaatgag cagaaaataa tgcccatttc agccagattc tccctgaatg tggttttcac
    23581 tgaaatacta tccatgacag catccacagc cattcccgac aatgctttct gttcgtcgag
    23641 cgggatgccg agtttatcaa aggttttgag taattcggga tctacttcat cgagactttt
    23701 tggtgcattc ttgcgcggtg cggcaaaata tgatattttc tgataatcta tttcgggtat
    23761 cttcagatgt gcccaggttg gcatctgcat ggtaagccag tgccggtagg ctttcaggcg
    23821 gaaatccagc aaccattccg gttctttctt tttggccgaa atcagccgga taacatcctc
    23881 gttaagtccc acagggatta cctcggtttc aatatcagta ctgaaaccat acttataatc
    23941 gctttgggtt atctcatgta atatatcgtc tgacatagat ttaatagaat aaaatgcttg
    24001 agtaataacg tgcaaagata acatattgtt tttaattagt gagattttaa tcctagacta
    24061 actctatatc atcagattat caacgcgttg aaaaacggta ttttgaattc ttcaaaaaac
    24121 attataaact tttaaggtat ttatctgttt tactgagaaa aaaatattta catttgaaac
    24181 gagtcgcgat atatcggggc aagctaagca acaattataa agaaattaaa aagatcgtat
    24241 gaaatatatc ggagcccatg taagcgcgtc gggtggtgtg gaaaatgcac cgctaaatgc
    24301 acgtaatatc ggtgcaaaag cttttgctct tttcaccaag aaccagaaac agtggcagac
    24361 atcgccttta acggcaaaaa gtatcgaact tttcaaacag cgctgtcagg aatttagttt
    24421 ctcaccgtct cagatactgc cacacgacag ctatctgatc aatctcggac atccagaaac
    24481 cgaaggactt gagaaatcgc gtaatgcatt tctggatgag atgcaacgct gcgaacagtt
    24541 aggccttgac cggctgaatt ttcatccggg aagccactta aacacgattt cacaggatga
    24601 ttgtctggcc cgcattgccg aatcaatcaa tctgacgctg gataaaacaa caggtgttac
    24661 tgcagtaatt gaaaacaccg cgggacaggg cactaatatg ggacatacct ttgagcaaat
    24721 tgcacaaatc atcgatcagg tgaaagataa aagccgtgta ggtgtatgta ttgatacggc
    24781 tcattcactg gcagcaggtt atgaacttcg tacactggaa ggatttactg aaacattcaa
    24841 aagatttgac acaattattg gtttcaacta tctgaaaggc atgcacatca acgatagcaa
    24901 gaaagatctc ggatcgcgag ttgaccgtca cgacaacctg ggtaaagggc tgatgggact
    24961 ggatacgttc cgctatatta taaacgattc gcgttttgat aatattccat tgatacttga
    25021 aacccctgat gaaactttgt ggactgaaga aattgcgttg ctttattcgt tcgattaaac
    25081 ctttcaataa taaataataa aaagacaaga gccgctatat tgcggctctt gtgttattta
    25141 agtattgtcg taattacaga attaccttgt atgttttgcc tccggtcttt accagatata
    25201 tggcattttg tcggacaggt atttgtacga gttcaccttg cgattttaag ctacatatcc
    25261 tcgagccatt catggtaaac acattcacca tttcatcatc agacaacccg tcaattataa
    25321 tatttgaatt atttgtgtaa accttcacat cgcttattcc aataacaggg gaagccgtca
    25381 atgattcgta tgatatggta agagtcgtat ttttcgttac cggtggcaca gtatattctg
    25441 aatttgatgc ttctgttgta atgtctgagc cattatagat tattgaatta attttccatc
    25501 ctgcttcggg tgcaatactg aaagtcggcg tatctccatg ataacacaca agatttacca
    25561 caccatttat agcacttttt attgacagat tgtattttaa cttctcaaaa gttacggcaa
    25621 gcgttttatc cgaggtaatt aatggtgtaa catacatacc attaattact tgtgttgtca
    25681 caaacgtgcc accatatatt accgctgcta ttctgtaacc aacatcagga attatagtga
    25741 atgttctggt acttccatta tttgtagtca aaagtgtatc actttttagt acattgttat
    25801 tttcctttat tattccacca acaccaaaga caacttttaa tttgtaggta tttgaagttg
    25861 attggagttt gaaagtaaca tttaatgtgg tgttagcaat aatattcggt gttgtaaact
    25921 gattattaac taactgattt ttcatatcca aaccgcctaa tgtcaaagtg gcaacttcgt
    25981 accctgcatc gggttgaatt gtaaaagttt tcgtagaata tgcctcaaca ttcactgaag
    26041 cgttattcga aagtactgta agaccatctt tcactactcc tcctgtacct gagttgattg
    26101 taatgctgta atttattttt gcaaatgtga cattcaatat ggcatttcct gttactgatg
    26161 gtgtcgtaaa ttggccattg cttatctgac ttttcacatc gtttgcattg tatgtcaaag
    26221 tagctacttc ataaccagaa ccaggtaaga tactgaatgt tcttgatgtt ccttcctggg
    26281 cttgtattat ggtattattt gaaagtgcta tattgttttc tttaacaccg cctcctgatc
    26341 ctatctgtat ggtgatgtta taaacttgaa gtatcttttt gaaagtaacc tttaatgtat
    26401 cgtttgaatt taagggtcct gcagaaaata cattattaac aatttgggat tttacatcaa
    26461 caccattaaa tctgacagag tcaagttgat agccttcatc aggtattatt gtaaataatt
    26521 taacagcatt aatttttgct gacaccagat cattattatt caacagtaaa ttattttctt
    26581 tgatagtacc atttaatccc tttaaaattc ggacagtaaa tacttctgtc gattctttga
    26641 acacgacttt aagatttgta ttactgttga tggcaggggt agtatattga ttgttagtca
    26701 actgtgattt aacatcgttt ccagcatata taattgagtc aacaacatat ccctcctgag
    26761 gaataattgt gaacgtttta tgggcatttt gctcaacaac taacatcgta ccgttggtta
    26821 gctctgctcc attctccttc accataccac ccgcattggt ttgcagggta attttatagg
    26881 taattttttt aaatatggct ttaaggacag catcagtttt aatagaatcg gttgtgtaaa
    26941 gattattcgt gagcggcagg ctacaaatag ttccaccaaa taacaaactg tccaacgcat
    27001 agttctctgc aggtacaatt tcaaatgttt tggttaatcc tttgtaaaca gctaaaacag
    27061 tatcattttt cagaatacta ccattgtaca taattgtgcc acctccggat tgaggatcaa
    27121 tcttaattgt aattttttgt gcagtatgaa atttgacatc cagcactgaa ttaccaacaa
    27181 ctataggagt tatatattga ttatttacaa tttgggaagt cacatctaca ctattatatc
    27241 gcaaagtatc aaccacataa taatcatctg gaacaattgt aaattttaca cttgaatttt
    27301 tgtctgctaa aaaagcagtt cctgactcaa tgattgtcga attaactttt acctttccgt
    27361 ttgtgccaga attaactata atattaaatg taccggcatc ttctgaaatt aaactaaatt
    27421 gcttccagta atctgctgcc tgatatgcag atttagaacc tataggaacc ttaagctgac
    27481 aagcggactt attcacaaat cgaaacgtat tgttataaat agttggtggt aacgaattaa
    27541 gtgaaataat ctttgtcaat ccagaacagc tataaaatgc ttcatcacca attgaagtta
    27601 aattctttgg caatgtcagt tcggttatct tactgcattc agtaaatgca gaaccctcaa
    27661 tacttgttac agatgccgga attgtaagac tgcccgtaag tccggtacag gtagcaaaag
    27721 ctccattagg aatcttcgat agatttcccg aaagtgtaag atttccatta agacctgtac
    27781 agttttgaaa agcataactt cccaatgtaa caacagactc tggcaataca agtgaaccgg
    27841 tcaatccaac acaatcctta aaagcagaat tacctataga tacgacattt gaaggtaata
    27901 caatacttcc agcgagcgat cggcatccat caaatgcatt atttccaatt gtttgtacat
    27961 ttgaaggtaa aatcaaatca cctgtcatca gtttgcagtt atagaaagct cctgttccga
    28021 ttgtagtcaa accagaggga aggggcacat gtgaattaag attaacacat gaattgaagg
    28081 cattgtcgcc aattaccatg acagactcgg gcagtttaac atcaacaata ataccctttc
    28141 cctgaaatgc tccggaaggt agggtgttcg cactaaacgt cgactcggac aggtctattt
    28201 ccattaaaat cttaagactg tctctgatca aataaatatc actgttattt aattgtcctg
    28261 ttatttttaa tttagttatt gatgacaacg gaacctgggt ttgagaagta aatgcacttt
    28321 tcaaactgcc tgcagaagca ttattgattg taatcaatgt acttgaccca atgtttaaaa
    28381 gtttccatcc atttgtggat ctgtatgtgt taattgattg agcaggaaca tataccatcg
    28441 aaaggtaagt aaatccaaac ggatcgccga gcagtacagg aggaacagta ttcttactgt
    28501 caataatttt taaactatga cattcataga aagcctgatt accaatttcg ttgagtttgc
    28561 tcccaaatgt tatgctggtt attgcatgac agtattgata tgcagaagct ccgattgatg
    28621 ttacattatc aggaataata aagttattta aattagtaca tgcatagaaa gcgttattcc
    28681 caatggttgt taaagatacc ggtaaattca catcagtcaa attagaacaa tattggaatg
    28741 caaaattacc aattgcattt atcgaagatg gtaatataac cttgctaaga gttagcttac
    28801 ctatggttac atctttgtta aatgcatatt gaggcaatgt attgtcggga tattcggcaa
    28861 aaccccacga agtcgggaac gtaccatcgt taccactgta agctacaatc gagacattgg
    28921 tcaggtcgag cacagacaat actgaaagtt catcacgtaa acatttaaaa tctctggcat
    28981 caagatttcc tgtaatagtt aaatctgtaa cagtggtctt ttccgttgca gttaagagtg
    29041 aagttaaaga accggcactg gttactgata tcgttttaga tacctgtgcg actgattgaa
    29101 tcgtaaaaac taatactaat aatagcaggg ttaaatactt tctcatataa atttcacatt
    29161 aataaattct aaatcaaaaa acaaacttat aaattttatc aggtaaatta ataattgatg
    29221 tacaattttt ggttatcctg tatttattca ctggcaaata tacaataata atttataata
    29281 attatcaact ccgttcaact caaacattcc agttctgaat atacggctta aaaaagggtt
    29341 taatcgttaa tcgtgtgaaa taagaaaata atctaacacc agatatagca aaatttatag
    29401 ggtcagagac ccgtaaaatt tgtagaaacg agaatcaccc gcaacccata agggtcggag
    29461 acccgaaata taaaataacg ttgaatgggc gtaaatacaa tgatattccg gtgcgccgca
    29521 ccttaaaaac aactcgctac gctgtagcta caaatgtttc gcggctctgc cgccaccgcc
    29581 cccctcctaa gcataacctt catgtcatac tgtcattctg agcgaagcaa agaaactaca
    29641 cattatctcc ggtcaacagt gattcttaat aaaataatat ttcacacgat taacgtttaa
    29701 accaaaaaaa gaaaggcgtc aaaatgacgc ctttctttat atttaatcct tatgattatt
    29761 ttcttctaat acctgaagga cctactgtct tatcaacagt tacagtaacc caagtattta
    29821 cgatttcacc ccaataatat gtaattgtaa caggtacata caattcgaat gaattaccga
    29881 ttacgttacc attattctga tatgtcaggt atccatattg agctgcaggt gatacacccg
    29941 gtacagaagc tgcgtaagca acttgcagac ttgggaaatt gctgagtgct actcttgtac
    30001 cgttcaggtt agtttcaatg gatttagttg ttgacaaagc aatgttagta actccatagt
    30061 aaccgtagag gtttggataa gatgcaaaag agtaatctct ccagtctgac aaacttacca
    30121 tcttagtaat tgggatataa gtattagttt gaccaaatga tttaccgtca acaaaatgat
    30181 ctggacttaa agcagcagct ttaattggac gtacaaatac tacgtcgaat gtcggtttac
    30241 catttacagt tattggctgc aagaaattga gttcctggca atatttagtg cggataccaa
    30301 tacgtgctct gagaacttct ttatcatagt taagcaacaa cttagctgtc gtagaatttc
    30361 tgttaagttc aagaatatcg ccaacattag ctacgaaagg attaattgta gcaattactt
    30421 ctgaaccgtt caataactgt ttaccatctg tgcttggagt aaattgataa ttaacaccat
    30481 ttgctgtgat tacaggctgg cttatagctg gctgaccaac tacattaggc acaaagtaat
    30541 attcgtagtt gctgaaacct ttcaaactct tatctgcatt ttgcaggaaa gcctggctaa
    30601 gatcattctt gatagtacag tttgtagttg tgctttcatt tacatccggt acattaacgt
    30661 tatgcttaac ttcatcaaat acagttgcat tgttagcgcc attcatatca taccagtact
    30721 tcttaatcaa actaccttca gcgatattca aatcagccgg tttggtgaat ttgcgagtaa
    30781 aggtgatagt gattttcgga tatactgttg gcaaactagg aacatattct acagtttgtg
    30841 taaatgttgc cggatttgca gtcatcaatg cagaccaaat ttgctgtgca gtcaagttcc
    30901 attccaataa ccaagtatct gttgtttgtc catcaggatt tttaatttct gacactgcac
    30961 ctggtacagg aacgagtgtg taaacagcat ggaactcttc tttgctcaaa ccaactgcat
    31021 tgtacagttt ttcattcatt tgttgaacag tagttcttcc tgctacatct gcgcattttg
    31081 cttccatatc agcaaaagta aacggaactg taataggagt tggcagcggt ttttcagcca
    31141 tatagatttt tacaaaacca agtaataccg gacatgaagg attttttgca ttatacatcc
    31201 atacgcgtac tattggagtg cggccctgtg cagcagcaat ttcagcctga gtaaatactt
    31261 ttgcttcgat tgttcctttt gcagcgtcgt taattttaat aaactgttgt tggtcggtgt
    31321 tgttagaacc tctgttataa acaattgtat taccgttttc atccaacaaa tcgaatttat
    31381 aattcaaacc atattttaca atgtcaaaag gagcatgaga gaagattgct ttaacttcgt
    31441 taatcaggtc aagagttttt ccataaggta aagtaactac ctgaggagct gttgttgttg
    31501 cgttatcaac atcttttaca gataaaagtt tagcatctgc aagtgtcaga ggaaggatcg
    31561 aataagtcgg agcattatta cgtgcaagat atacgttttc ctgagcgtac atattctctc
    31621 cgtaaagtct tacataatca gatgtaataa cacgattagc aggatattca gtgctaccta
    31681 caacaaccat tttaccacta ttgtcaagtt gaatagcatt ttcctgaaca gctttctgtg
    31741 aaagtggtat ctgaagagca atcgaacgga atgtttcgag tgatccacct acataatttc
    31801 caccttcttc accaattaat gtattgtaat catttactga aacttttaca tagatgatac
    31861 cagtagttgc atcgatatga tcataaatag ctgcaacttg tactgctcta tcattcgctc
    31921 cctgaaccgg caaataataa tcgttagtaa ttgcattaga accttttctt actacaaatg
    31981 ccatattacc agtttcgata tcagcttctt tgataaacga agggttgaga tgatatgcga
    32041 ttgtaatagc aggagtaata ggatcacatt tagacattaa tgatgaaaaa ttgatagctt
    32101 caatgccgtt aacatagaat ctgcttaatt cggtacctga aaggtcgcct gcataaacaa
    32161 gactacttaa acgactgctt aatactgcaa aattaagatg catagcatta gcaacagttt
    32221 tcagattatc attgataagc gcaagttcag ttctgattgc agtaatatca gcagtattct
    32281 ttgcaatgtc agcagtgttc tttgcaatgt cagcagtgtt ttttgctacc gctgtttcaa
    32341 gagcaactgt tcttgctttc agagcgtcaa gttgagcctg caaatcagca attttagctg
    32401 tattagcatc aatcaaagct ttctgtgctg ttaagtcaag ttggattttg tctataactt
    32461 ctgatttacc attcttgatc gacaggacac cttcaagaat tgaaagacgt gcttgcacat
    32521 cagctttcaa agcagcaaga tctgtaatgt tttttgcaat atcagcttcg ttttttgaaa
    32581 ttcttgcttt ggcttcatcc agttgagttc caagaagatt aacgcttgtt tgcagtgtgg
    32641 taactttagc acctaatgca gtaagtgtat tttcaatttc caggatttta ccatccagac
    32701 gggttgcaag tgctgttaat tcagctttag ttgcagcagc atccactttt gttttcagtg
    32761 aagcaagatc agcatctact gtagctttgt aagcattgaa tgcttccaaa gtaacaactt
    32821 tgctcataat ctcagtttta gcagcggcaa tggcagcagc aacttctgca tcggtagcac
    32881 cggtttgttc cagagtagtt actcttgcct tcagagcagt aatgtctgta ttaagagtag
    32941 taatctgcag ctgcaatgca ttttgtgttg cagtgttgag agctgttaat tcagtcttca
    33001 agcttgtaag atcggtgttc agcttgtcga tatcctgatc gtaagattta caacctgtaa
    33061 atgttacaag cgatagagcc ataaccatag cgaagactct ggttatgctt ttcaatttaa
    33121 tctgaaatac ttttttcata aatttttttt tttgataatt aaatttgttt tatagttata
    33181 gttattcatt ttttactggt tcacaataca gtaataagac gccacagata tataaaccct
    33241 ggcaatcaat aattcgatta gattttcctc ataattaatt agttttaaat atttactact
    33301 ttattccctc gtatattaaa aaaacctaca aatatgctaa aaaatccttt aattcctgct
    33361 tgtttatact attatttata cttaacaaaa aaataacaac tcatcatcta attcaaaaaa
    33421 tcaactacac aaaaaaactc aaagttatct atgaaattaa caaaaaacta tacatgcaaa
    33481 ccctataaat cggattttta caaagtattt aaatgcaaag atatataaat catacagaat
    33541 tcaaaatatt tttacctctt tttatttgaa tattttaacc ttgtcggttt aaataagtaa
    33601 ttcattctgt tataaccctg ctccaaccca tatatacagc cttttgaagc aattttaaat
    33661 gcaaagatat aataatcaaa cggatttcaa aataatttac ctattttttt ataaaatatg
    33721 ataatctgtt gatttgcttg tagtatcctt tttattctga atgagttaac cagcaaaatt
    33781 aagttttgat gcataaactg cagacaaatg aatatctaaa tcacccaaaa aaatcatcgt
    33841 tatcgtattc aacatagtat ttgcatctgt tcttggaatt aacaccatct gtttgttagg
    33901 cagaatcaag cgattaccta aatgttactt aaataatagt atattataac tttcagcaac
    33961 aatataactg caacacaaaa aaacagaaat aattatatta gaaaatgata acgtcaggta
    34021 ttcatttttt aaatataaag aagcatataa accaaaatcc ctatctattt agttcgcaca
    34081 ctaaataacg tgagacaaat ttatggtatt ttttttaatt tgcaattttt ttataaaata
    34141 tttacgcatc aaacacatta aaaatcaaca ttctgtatta tttctgcttt tatagcatat
    34201 aattctaact ttgcttcact attcaacata atatacaaaa atcataccag ataaaaaaaa
    34261 taagccatta caattataaa gaattactta ttaacttatg caaattatta ttgtccgata
    34321 aaaacaaaaa aaataatgtc gctccgatgg gagcttttgt tgatgggtgt aatctgtttt
    34381 ctaccataat gtcgcaccgc tggtgctaaa aattaaagcc tcgtagaggc aaaactatgg
    34441 tagaaataat atgtaacaga atataaagcc tcgtagaggc gacattatgg caaatcacgt
    34501 tttataagga cttacaggat ttttctaaaa tgtttaccgg acaacaatat gtgcaaatat
    34561 attttcttct ttcaaatata aacatttcac cagtatattg ctatacccga cttaagccta
    34621 tcggttattc caacaattca atttttaatc aatgcaggcg tatttgaata gtgatacaat
    34681 atttaatgta tatatatata tatcaaaaaa aaataatcgt acatattatc taatctgcaa
    34741 tcggcacata cttttctttt aatccgtttc cattatcaat aatatagaag agcgaaaaac
    34801 aaaaaaataa caataaaaat attaattttg ccatcggcca caacatatgt ttgataaact
    34861 tcctttacat cacatgtgag tttcacagca tcacatttgt cataactaaa atattaaact
    34921 aaaatgagca aatcaggatt atctttcaaa tatcttatca ctatattgac aggtgttctg
    34981 atatcatgct gcaatacaga atcacctcac ttaaccataa actttgataa aagagcatcc
    35041 atgccgggaa acggacgctc ctctgccgtt ggatttgctg ttaacggtaa gggatatgtt
    35101 actttgggaa ggaatgcaga caatgttcag ctgaatgatt gctgggaata tgatccacag
    35161 ctgaatacct ggaagcaaaa atcctcattt cccggaattc caagggtaaa agcttctgca
    35221 attgtattga acaacaaagc atacatcgga ctggggcaca acaatgctgt aggtgtatat
    35281 aatcctacct cttgtctgaa agatttctgg gaatataaac cggaaacaga tacctggagg
    35341 agattagctg attttcccag taactttacc gatgcttgta ttagttttgt atataattat
    35401 gctatatata taggtattgg tttcagcgaa tcatcatacg ggagtgaact ctggaaatat
    35461 attccagctg aagacaaatg gattcgactg aatgatttta caggaagaca acgctttggt
    35521 gctgttgcct gccagaacga ccgtcatatt tttttcggaa ccggataccg aacgggcaac
    35581 ctgaatgatt ggtgggaata tcatcccgaa tctgacagtt ggaccgaact tgaatccatg
    35641 cccgacaatg gaagggtaaa tagtgtatcg ctttgcattg ataatcgaat ttttgtagct
    35701 acaggcagat attttggcgg taatctgacg ggtggacatg tcaagtccga tctgatggaa
    35761 tacggactat ccattgatgg ctggcaccta agaggcgaaa taccggacgg taaccgcgaa
    35821 aatgccatag cttttgtcat tgacggacga ggttacattg gattcggaga aaacgacaat
    35881 ggggtactga atgatttctg gtgcttcaaa ccctgaaaat gataaaataa tgaatcctca
    35941 gaccctactc aatcatatcc attgtaattt ttccaaaaaa ttacttttag tgaacttaac
    36001 actcatctca atggctgtat tttcccaaaa agacagcatc tgtttcagca cagaaattac
    36061 aggagtttac tctacgggaa aatatgctcc tttttggctc caaagcaacc ggtatggcag
    36121 aatttcttcg tctccagcaa gtactttttt atcaacaggt atcgaaaaag catataacaa
    36181 accacagagg ttgtttgatt atggcttcaa ggctgattta ctgacacgaa ccgataaaac
    36241 taaaactgaa ttgttcccgc atgaactctt tgtaaaagcc cggcttgcgg tttttaatct
    36301 taccgttggt tccagggaac agatttttgg caatcaggac tcgactttat cctccggcgg
    36361 gcttctattc tcaaaaaata cacgtcctat gcctgaaata ggtgccggaa tagaaaattt
    36421 tactcctgtt cctttaaccc tgggttacgt ggagttaaaa ggtggactga gacacggctg
    36481 gtttactgac aatttatata ccagagaaat actgctgcat cacaaatttg cttatatccg
    36541 acttggaggg aaacttccgg ttcacgtttc atatggtatt gagcatgtgg ctcagtgggg
    36601 aggtacagat ccggtttacg gacctttgcc cggtggattg aaaaattatg cacgaatatt
    36661 tctggctaaa tcgggaggag aagacgccag tgtaagcgac cagattaatg ttctgggaaa
    36721 tcatatcatt tcacaggggc ttaaattaga gctgaatgta acaggcataa gacttaacgg
    36781 ctactggcaa aatatatcgg aagaccgtcc catcaggttt atcggatcaa accggattaa
    36841 taaaccggac ggactttggg gaatttctat aaaaaacagc agatttccct atatccgggg
    36901 cgttttgtat gaactgctca ataccaccga ccagtcggga ccttatcacg ataaagacgg
    36961 attgatatac ggaggtacgg acgggtactt cggaggttct tatcctaccg gatggagcta
    37021 ttactcccgt actatcggga caccatttat cacttcaccc ctgtacaatg aaaatggcac
    37081 attacatacg accaatagcc gtgttcgttt gcatcatttc ggcatggaag ggaatatccg
    37141 tggggttgaa tacagagtac tggcctcctt cagcagaaat tacggaacct atggttctcc
    37201 agtgaacatg acaaacaaat cttttttact ggaagttcag aaatatattt ccctgctgtc
    37261 gggttttgaa ttgagctgct cggctggtag tgatgccggc aatttatttg gcaactcggt
    37321 gggtgtcctc ttttcaataa ggaaaaacgg attgctgctt aaatattaaa aaagtttccc
    37381 ggtttatttc cggaaaactt cttctgtacg tttatcaatc tgacagtgta aaccgtcata
    37441 gttgactatt tttcatttct tttctgaaca ttatagaaaa tcttcatctg gttacccaga
    37501 atttttgtaa agcctttcac atcatcagct gtccaggctt tgtttacttc tccgtattct
    37561 ccgaaatcgg ttttcatcaa atcaaaatcg ctttcgacac ctacaagtgt ataacagtac
    37621 gggcgaagtt tgacaataac acgtccggta acattttgct gtgaactttg caggaattgt
    37681 tcgatatcac gcatgacagg ttccaggtat tgagcttcgt ggagaaacat tccgtaccag
    37741 ttgccaagct gatctttcca gtactgctgc catttggtga gtgtatgttt ctcgagcatt
    37801 ttatgagcat tgataatcac cataggccct gcggcttcga aacctacccg gccttttata
    37861 ccgatgatgg tatcgccaac gtgcatatcg cgtccgatgc catatttatt ggctatttct
    37921 tcaacctggg tgatgagttc gactttattg gtgtttttca ctccattaac cgcggctatt
    37981 tcaccctgtt caaaatcgag tgtcagcgtt tcttccgttt gcttaacaac ctgactcgga
    38041 taagcgcttt cgggtaaagt ctgttcagat ttcagggttt ccttacctcc gatacttgtt
    38101 ccccaaagtc ctttattgat ggaatattcc atttttgtaa aatctgctac atatccgtgt
    38161 tgcttcaggt agttgatttc atactcgcgg gtcaaagtca tatcccgtgt tggtgtaagt
    38221 atctttattt cgggagctaa tatttgaaaa gtcaggtcga aacgcacctg atcattaccg
    38281 gcgcctgtac ttccatgagc tacataatcg gcacctattt ccttagcgta attgataatg
    38341 gcaatggcct gaaatattcg ttcggaactt accgaaatcg gataagttcc attgcgcagc
    38401 acatttccga agaccatata tttaatgctt ttttcataat actcctgcgt aatatcaagc
    38461 gatacatgtt ttaccgcgcc aagacgatat gctttttctt caataacctg caaatcctgt
    38521 tcgttgaatc caccggtatt tgctaccgct gtatatacct cgtatccttt ttcttctgct
    38581 aaatatttgg cacaaaagga agtatccaac cctccgctaa atgctaatac tactttttct
    38641 ttcattgttt tattcttttt ctttttgttg tttctgtaga agaatttttg gagacatcca
    38701 ttctcccata acctgaatca gtttccacat tttatttttt cgggttttct tttcgctccc
    38761 gttattttcc tgccatgccg gatcataaag cataccggta caaagacaat gcttccgtcc
    38821 tgtgcgctgt aatatatcat agttcacaca actctgacag cctttccaga atgcttcatc
    38881 atttgtaagc tcagaaaaag taacagggcg gtaaccaagt tcggaattaa tcttcatcac
    38941 ggcaaggccg gtggtaagtc cgaaaatctt ggcatccgga taacgttcgc gggagatctg
    39001 aaatgctttt tgttttatcc tcttggctaa accatgccct ctgaatttat ccacaacgat
    39061 taagcccgag tttgcaacaa attttttatt tccccaagtt tcaatgtaac aaaatccggc
    39121 aaactcttcc cctttaaggg caataatcgc tttactctct ttaatctttt gctgaatata
    39181 ttcgggtgag cgtttggcta tacctgtacc ccgaattttg gcagcttcct cgatggtagt
    39241 tagtatggtc tgaacataag gcaaatgact tgtatcagcc aactgcacta ttatgtcatt
    39301 attttctgta tttaatttgt tcattttggt ttaagaactc attctaattt ttttcgtgaa
    39361 aaatcaggtt tattattttt gttatttcat ccctggctac tccttcgcga ggtatgagca
    39421 aaattgtatc atcacctgca atagttccaa gcacttcgtc ggtagcacga ttatcaatat
    39481 cccaggcaat ggcacttgca taacccggct tggttttaat cacagcaagt tgcccagaga
    39541 attccaggtt caaaactgca ccgtgtgaag taaaattgtg cgcattcagt tcgcctggaa
    39601 taggtttaat ctgaggaggc aacatatatt tatacgctcc gttcgacaga ggggttttag
    39661 caactttcag cactttgagg tcccttgaca acgtagcctg agttacgtca tagccttttt
    39721 cacgaagaag ttcgagaagt tcttcctgac tacctactac ctgcaaccga agcatttcag
    39781 atattacgct taatctctct ctcttatttt tcatttttgt ataattattt aattcaggcg
    39841 caaatttaca actttatgca gatttaccca aatttatcag tatatttttc caaaaataat
    39901 tttagaaaat gtaaatttat gcaatagatt atgtgtatca ttctgattta tagataaaaa
    39961 aaat
//
LOCUS       DAOPYB010000010        37824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_976_length_37824_cov_9.522969, whole genome shotgun sequence.
ACCESSION   DAOPYB010000010 DAOPYB010000000
VERSION     DAOPYB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 37824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37824
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_976_length_37824_cov_9.522969"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..3302)
                     /locus_tag="PKH58_01660"
     CDS             complement(135..3302)
                     /locus_tag="PKH58_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNX87762.1"
                     /translation="MKRTGLTRLILLSFLFSGLMVNAQKPYKAYLVSNAHFDTQWNWD
                     VQTSINQFVRHTMDRNFLLFEKYPDYIFNFEGGIKYKWMKEYYPEKYKRVKEYIRQGR
                     WHVSGSSWDATDPNTPSPESFFRNILLGQEFYRKEFGIQSTDIFLPDCFGFGYALPTI
                     ASHCGLIGFSTQKLQWRHNPFYKNTRNSKLPFYTGIWKGLDGSQIIASLNSQNYVHSW
                     DGQDISNDNEIRELADGSVDKMAYKYYGTGDRGGSPTIPSVISVEKGIKGNGDIQIIS
                     ATSDQLFKEFLPFKNHPELPVFDGELLMDIHGTGCYTSQAAMKRFNRRNEQLAVASEK
                     AAVLANYLTGSAYPSDEIDESWKRFIWHQFHDDLTGTSIPKAYTFSWNDELISQSCFA
                     DVITSSVGKAAKEMNTNTKGTPIVVYNPLSTAHKGLVRAEISMPQGNQDVRVFDQNGK
                     EVNAQLVKNINGNAEVVFCADVAPIGYALYDVRTGKAKKVSQLKVSANTLENRIYKIV
                     LNSNGDIASLIDKRNNKELIQKGKTFRLALFTNNESYEWPAWEISKKTIDATPISITD
                     GVSISIAENGPACASLCVKRSYGDSKFTQYIRLTDGAADDRIDIVNEIDWRTTDALLK
                     AEFPMSVSNPKATYDLGLGYIKRGNNENKAYETCAQQWADITGDEGYGISIMNDCKYG
                     WDKPSDNTLRLTLLHTPKTSDRYTYQSRQDFGHHTFTYSIIGHENQPQFASIPSKSEE
                     LNNPLMAFETPRHKGTLGREFSFIKLSTSQMALKTLKKAQNDESYILRFYETQGKDAK
                     NVTVEFPSEIVSAVETNGVEHEIGKADFNGKKLTFSTTAFSPKTFAVKLKGNTVKSQP
                     IHNTPVELPYTDNAFTYDAFNEAGDFDKDGCSYAAELIGKEIVSQGIPFRIGDMETKN
                     VVKCKNNIIDIPNDRKYNKVYLLAASTDGDIKADFKFGYIVIKCDIPYYSGFYGQWGQ
                     TGFTKSFVKNVPLAYVGSHRHSSKYGNEAYTFTYMYRICLEIPEGATQLQFPDDNRVA
                     VFAVTFSDNYADDVKAVSPLVSTLY"
     gene            complement(3584..5974)
                     /locus_tag="PKH58_01665"
     CDS             complement(3584..5974)
                     /locus_tag="PKH58_01665"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX87763.1"
                     /translation="MKKLLFFSFLLLMFGNIQAQKLTMVRNSYIAGEPISVKFTGSTS
                     TKDWLAVFHQTTVPGSMNNEGWVYTSGTQDASSTLIESGAVIFSSGVTEPGIYKVCLL
                     ADDGYSVVTSVEFNVVTPVSGLVGEWKFDNPGNLVKATVGNDLQTNGTLISSVAGPTL
                     TDGAVRVGVGSNFTLVHGITPFPNALVNTYSFVFDFKVSQLNKYYSFFQTSPANNNDA
                     EVFLGKLGNVGVGATGYSSQTVRPNTWNRAIIVVNNGSEYSVYVNGVKGLTGYVQPVD
                     GNFSLEPTLLLFGDDSGEDNEIDCANVQLYNKALTENEAQSLGGYVQDFRTLCSFLSK
                     PYVQNVTSDSITVMWESDFIKAGSNKVNYGTNSDASGGAQIIATAYPSGANTYIQKAV
                     IKPLQPGTTYFFRAISNSSPNMIQSVKTAPKDPNSVFTVGIWGDSRFLNPFTSMATYL
                     VNNLAPDFCISTGNITINGNDSVELRKVFIPAVLETVGTKVPFIESLGNLDVDASLKG
                     ADIIRNYLVQPRIYNSDASKVSGSFAYEYGNSVFISIDPTRYNTDLARDGWLETFLKS
                     DVSKKALFRFIFINNAPFYERCQKYEQEVVKTNIPLLSKKYGVTAVFSGRMHGYERGV
                     IDGVQYITQGGCSNMDVDSIVGPTIYPHIVVGTNKTDNPANFNNGLMNHLLTLEINPE
                     FATVNLHYFDGTGNYKGIIEIVQMAPRAPENLDAVSTPSELGFAISPNPTKGIIRINA
                     SGNVDLAVFNLQGVKVAGQLNVSPNTEINLSNLAKGAYLVRLKAGQNEFTKTILIN"
     gene            complement(6022..7902)
                     /locus_tag="PKH58_01670"
     CDS             complement(6022..7902)
                     /locus_tag="PKH58_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX87764.1"
                     /translation="MKHTISILIFLFLVVKLSAQTVSTDKSTYKVGEAITVFFSGATS
                     AKDWVGLYVSTSTPGPASPSIDWYYIDGTKTGSSLVSNGYVVFQNGLSTEGSYKVCFL
                     SNDGYNILKSAIITISNSVSLAAFDAAPTFIVPGGTVKFTDQSANSPTYWLWSFPGGT
                     PSSSTEKNPSVSYPVEGVYDVSLSATGASGTLQIQKNGIIRVSNQTLSVNLKVMQFNI
                     WREGTSVANGLTLIRDVINSVNPDMVCFSEVTNSSGDWTTKIVNELATLGKTYYRGYV
                     TGSDVSLISKYPITSSGPLVGERTVPFTVNVNGASVVVCPSHLDYTYYSTYLPRGYAC
                     GGSGKYAGWNAFSPFVPETDLAAIQAQNLDSRRDEQIAAFIDYMKNETRPVLLIGDFN
                     EPSCLDWTARQANLYDHNGVVYEWNTTRALKNNGFVDAYRQVYPDEVLNPGITWPAVA
                     TGVGSTSWAPLSDERDRIDYIFYKGTGVTATEATLVGPNACYVKNSATTEGNGNDVFE
                     ASTLPWPSDHKAVTATISFSFVPTSVSTVNKPDTKIWVFPNPTKGQFSLISSENEKIS
                     VSISSLSGQEVLNKKMDLLANQSSFFDIENAPAGIYMLNIFFRNQSQTIKLIKKTNNY
                     HY"
     gene            complement(7985..10114)
                     /locus_tag="PKH58_01675"
     CDS             complement(7985..10114)
                     /locus_tag="PKH58_01675"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX87765.1"
                     /translation="MKLFSYIILIFAFISTSLLKAEEKVNYARLVDTRIGTEGNGLAC
                     GFTFVGAAYPFGMMQLTPSFFSAHKGIVVNQISGAGCPNMGNFPVLAMAGDLQDSPND
                     MNGYPRYETINNSTAGYLSMTMKDKVICESTVSKRSGIIRFKFPDNASNGTVIIGSGI
                     SSTSVSNAQIKITSPNTCEGYAEGGEFCGYQTDYKVYFAAEFNREPQLRGTWINSDMR
                     DDRLKISGRNSGAYFTFNTENNKELEYKIAISYVSIENARENLCLDNKGRGFETMKND
                     AEKEWNRCLGKIQVQSGNSDRVKQFYTHLYHSIIHPNVFNDCNGEYIGSDFNTHKTVK
                     GRDYYTTYSGWDTYRTQCQLLAMLFPKESSDMMQSVVEFAEHGGGYGRWVMANIETGV
                     MQGDPIPIIISNTYAFGGRDFDTQTAYKHMKRGAMVAGTYSQNVEVRPGLHNYIRKGF
                     ENASLCLEYASADFAIGQFALQATNNKKDADYFMKRAGNWKNLYDPQTNWLRSRDTHD
                     MTWKKPDDDWREATKENYFWMVPFDLKTLIDTMGGKEAASKRLDSLFVRLNAGYDDHY
                     FAAGNEPDFQVPWIYNWTDKPDKTSEVIYRILNQMYTSKPGGLPGNDDGGSMGAWYVF
                     ASIGLYPMIPGIAGFSINTPQFENIKIDLSGKMLEITGGSSSPVFIKSVKINERKHTS
                     YWIDWETIRQGGSIEYKTERKNDVKLK"
     gene            complement(10195..11544)
                     /locus_tag="PKH58_01680"
     CDS             complement(10195..11544)
                     /locus_tag="PKH58_01680"
                     /inference="COORDINATES: protein motif:HMM:NF027705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4998 domain-containing protein"
                     /protein_id="HNX87766.1"
                     /translation="MKKKSWQIFIGLIITVFSFNACSDITDTQRKFLDIGEISYVGRM
                     DSVIALGGNGRAIIVAKNTYLRTATKCIVKWVDFQGASSEKTFEIKDYVTGNYTRMPI
                     EGLPEGDYDFYIYNVDDWGNKSLTVECHGSSYGKEYARIQPKIAVTAITVDDSNTAQI
                     TMSTSKMAVKFRLTYQGDNAEEKTLDVNGTGGKLMIPNWGDYENLQLKVTTYILPKDK
                     LGVDTLVLQEVTQTAKQTVIDFDVDKSRIVPMALTKNDDPGNSYSGKGIAALFDNADT
                     ECWGANISAPGHFCFDLGTKAYLSGASIVGRLDYPGWDVVKFEVWGRESLDDGKYGTT
                     GYYIMASSQAANFVTEATTRKWKKVGNGWFKYTSPRSNPQTSKCVFTEVDHSFKPRYI
                     MFRVMSVLTPDVVPVPDTQYFGNEGGFYSGDGVNSKNRAFCIKELNFKALGISYIIQ"
     gene            complement(11580..12776)
                     /locus_tag="PKH58_01685"
     CDS             complement(11580..12776)
                     /locus_tag="PKH58_01685"
                     /inference="COORDINATES: protein motif:HMM:NF027707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4959 domain-containing protein"
                     /protein_id="HNX87767.1"
                     /translation="MKKLILILTVVATLFSCTEENLLKPFGPEDTTAPGVLTNVSYKA
                     LPGGAKFSYVLPLDKDLLYVKAEYYIDGVKKNVVSSQYNTELTVEGLPEKKEYQVNLY
                     CMDKSENQSEPVSITIVPEESPVKVMRESLKCNVDFGGFRIEYKNPSKAELSIYFLRK
                     DSIAGKLVFYQAKVFTQAEGVYQVVGLPNITNKFGVFVRDRFGNTSSTLEFEDRPWRE
                     ELVDKSNFKYVGKPKVVDNDDWYSWSGRPQVLWDDVVGIWNFASTGPEGKFPHYLCID
                     LGDTVPVGRILLQQRGVFEGACPKHFDVYGAATLPAVNYASPLEGWTKLNDKTFEVVR
                     PSGRKPGEPATTEDVQAAEKGLMFTIDTPFPRPAIRYIRFKFIDAFDGQISVYASEFT
                     FWAQWK"
     assembly_gap    12842..12941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12942..14369)
                     /locus_tag="PKH58_01690"
     CDS             complement(<12942..14369)
                     /locus_tag="PKH58_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX87768.1"
                     /translation="MKRTIYSAICAVSLFLCASCSDYLDIVPDNIPSIQQVFDTRENA
                     FKMLYTCYDYLPRTANVFQNPALLCGDENWNPEFVDPNFYYYRNRSTEYIAKGQLDKT
                     DPLLNYWDGGDYSNLNQKFGDVEERTKIKSLWAGIRNCNTFIDNVDKVPDMTDDERAQ
                     WKAEAKVLKAYYHFYLLQMYGPIPFMGENLDISLSPEEVQREREPVDDVVSKIVAVLD
                     DAINSNDLLPTYAARRTELGRINLPIALAIKAKVLVLAASPIFNGNQKFSSFKNSAGV
                     PFMNPVFSQEKWKVAADACKAAITAAHDAGLDFYTFNQKMNSLTVTDTTQLELNLRGQ
                     ITENLNNTELVWGVGRQDVQRLIYYASCPLYALQIGARPLGFNNLHGATMNVVDQFYT
                     SHGVPIEEDKTYDYSDRFSLIRVPEKDHLYFTADTNARVPYYNTGREPRFYAYLGFDQ
                     GRYFSKEITDDHNSYVIHSKATEFAG"
     gene            complement(14381..17563)
                     /locus_tag="PKH58_01695"
     CDS             complement(14381..17563)
                     /locus_tag="PKH58_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX87769.1"
                     /translation="MSKLIQKKRYAFAVLLMLFSALCSGEISAQNIKVKGVVRDKSEA
                     ILGAKVVARSNPKTGVATDINGSFTINTKIGDVLEVSFMGYETKTVKVTGPEDLKITL
                     VEAQKTLDEVTIVAFGKQKKESVVASVSTVRPADLRVPASNLTTALGGRIAGLITMQT
                     SGEPGADNAQFFVRGVATFNNYSRGPLILIDNMELSANDLARLSVDDIQSFSIMKDAT
                     ATSLYGSRGANGVILVTTKMGVEGKTKLNFRYEKSTSQPTRTVEFADPLTYMILNNEA
                     IVTRHPQYNRRYSDDQIAGVEAGTNPYYYPSNDWLNLLFKDRAENQRFNMNLSGGGQR
                     VKYYVAAAMTSDNGILKVDKLNNFNNNISIKNVNLRSNTDIDFTPTTMGSIKFNANFL
                     ESSGPMYTGNQMYQRVMLSNPVDFPAYYQPDEINLNTKHRLFGGTQSYSFINPYADMV
                     KGYQQATQTNLVAQIELRQKLDFVTPGLSARFMASTTRQSVYSINRSYGPYYYTMSTY
                     NPITGYFKLNNFSKGNESLGFAGGNKSINSTIYGELSASYEKIINDMHNFSAMTVGTL
                     REVTTSDFDNDLQRSLPTRNIGISGRITYGYDHRYFLESNFGYNGAERFAKNHQFGFF
                     PSFGGGWIISNEKFWHKSSPITNLKLKATYGLVGNDAIGDAYDRFFYLDRVDMNKGLS
                     NDFGESGGGNSSGINFNRFANTSITWEKAAKMNLGLEITLVKELEFQFDVFRENRTCI
                     LINRTAIPTTMGLSDFPAADGKAPADVRANVGAARSSGFELTLTENHSFNKDVWLRSN
                     LTFTYAVSRYTDYEEMVPAGMDWLKTEGTYIGQQKGLIAERLFIDETDIANSPIQTFD
                     KVNDIPVMPGDIKYKDINDDGIIDSHDVVPIGYAGVPQLEGGWGVSFGFYGFDFNFFL
                     QGAARVSFMMDPTARVDWGSGSVGTAPFIDNHSLLKAWADDHWSETNRNCYALWPRLS
                     DTSRPNNEQASTWWLRDGGYLRLKSVEIGYNVPEKLIKKVGIQQLRFYGSGMNLLSFN
                     NFKLWDVEMGGSGLNYPIQKVFNLGLSLNF"
     gene            complement(18324..20570)
                     /locus_tag="PKH58_01700"
     CDS             complement(18324..20570)
                     /locus_tag="PKH58_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNX87770.1"
                     /translation="MDIYENPSLSIDARAEDLLQRMTLEEKTCQLATLYGSGRVMEDV
                     APTPEWKTAIWKGGIGNIDEQANGLGKFGSSISFPYAVGVQNRQAIQRWFVEETRLGI
                     PVDFTNEGIRGLCADRATLFPAQCGQGASWDRALIKDIAGVTAREANALGFTNIYSPI
                     LDLAQDPRWGRVVECYGEDPYLVGELGKQMVLGLQSNGLVSTPKHFAVYSIPVGGRDE
                     GTRTDPHVAPREMRTLYLEPFRKAIQEAGALGVMSSYNDYDGEPISGSYYFLTQILRN
                     EWGFKGYVVSDSEAVEFLNTKHKVAPTMEDAVAQVINAGLDIRTNFSGPHNFILPLRQ
                     AIVDGKVSLKTIDDRVLDVLKVKFKEKLFDNPYKGNEELSEKTVHSKANQAVSMKAAL
                     ESMILLKNDKNILPLNKDIRKIAVIGPNADEAKNLICRYGPANAPVKTVYQGIKSYLP
                     NADVLYAKGCDIIDKHFPESELYDVPLDSAEQQMINEAVEQARKSDVAILVLGGNEKT
                     VREEYSRSSLNLCGRQEQLLKAVFATGVPVVLLIIDGRAVTINWAKKNIPAILYSGFP
                     GEFMGDAVAHILFGDYNPGGKLPVTIPKTVGQIPFAFPFKPGSDTKGNVRVDGPLFPF
                     GFGLSYSTFEYKNLKIKYPVIGPLANNQLSCTIKNTGKLPGDEVVQLYIRDEYSSVTT
                     YVKNLRGFERIHLLPGEEKEVNFELTPQELGLWNKDNHFVVEPGTFTIMLGSSSEDIR
                     LTGSFEVK"
     gene            complement(20782..23079)
                     /locus_tag="PKH58_01705"
     CDS             complement(20782..23079)
                     /locus_tag="PKH58_01705"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX87771.1"
                     /translation="MKQRICLLSFLTLLSFCMSAQRDYTQYVMPLMGTQSEFALSNGN
                     LYPATALPWGMNHWSPQTATKNDERWYYSYNSHYITGIRQTHQPSPWTGDYGVFSLFA
                     TVGEKKFREDDRKSWFSHKSETSLPHYYKVYLADYNVTAELTPTERSCVIRFTFPETT
                     QANVVIDAFARASYIKVIPEENKVIGYSTERIGYENQRVPSNFRNYFVIYFDKPIRDN
                     SVWKEDTLINEKETEGRRSGAVVTFSTQRGETVQARISSSFISWEQAEQNLKAEVGGR
                     SFDEVCAGSKAVWNSHLSRFSVENAQLNDLNNIRTFYTCLYRILLFPREFHELDAQGN
                     IIHYSPYNGKICSGRMYTDNGFWDTFRAVHPFFNLFYPTQSKYILEGLANTYKESGWL
                     PEWAAPGHIDIMVGSNSTSVVASAYLNGVKDVDINTLWEGVMKNAFNAHPTIGSVGRA
                     GAKEYGELGYVPNDIGIKESAARTLEYAYADYCIYKLARELNKDPKTIETFAKRSLNY
                     RNLFYPKYNLMAGRNSKGEFRRDFNPFSWGGDFTEGNSWHYSWSVFHDPAGLATLMGG
                     RKKFTAMLDSVFTLPPVFDESYYGQVIHEIREMQIMNFGQYAHGNQPIQHMTYLYDWA
                     GEPWKTQYWTRQIMDRLYRPAPDGYCGDEDNGQTSAWYVFSALGFYPVCPVSGEYALG
                     SPLFSQLVITMEDNRKLTLNAQENNSENIYINKIDWDNKEFNKNYFTLSQLRKGGNIL
                     FQMTNKPNYKRGTTKESFPYSFSDK"
     gene            complement(23783..25336)
                     /locus_tag="PKH58_01710"
     CDS             complement(23783..25336)
                     /locus_tag="PKH58_01710"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNX87772.1"
                     /translation="MRKITLILSFLMTISAGALSFAAGQAKNNPEKKILTITKTDLFD
                     KIKGGWAAKTIGCTYGGPVEFQYNGTMIQDYVPIPWDDNRVKWYFDNFPGLYDDIYVN
                     LTFVEVFEKMGLNAQPADFATAFAHAPYPLWHANQVARYNLQHGVKPSEAGNWLNNPH
                     ADDIDYQIEADFAGLMSPGMPNSASEISDKVGHLICYGDGWYGGVFVGALYSMAFTST
                     DINFVVSEALKTIPQQSSFYQCISDIIKWHTAYPSDWKRTWFECEKKWSAENGCPDGV
                     FTPFDIDAKLNSAYVVIGLLYGEKDFFKTIDIAARCGQDADCNPATAAGVLGTMVGYK
                     NIPEYWRKSLDQVKDINFIYTDLSLTKIYDIGMKHALQVIAQQGGKVEKEQVSIRRQK
                     PKAVKYEKSFENHYPVKKISVNKSIKDKPKIDFEGIGVVLKGYVQCPDTNYVATVEVY
                     LDGKLAETAQLPVASVNSHRVDLFWKYQLQKSKHTVTFNWMNAKPDAQAIVTEVVIYS
                     DEPNKLIYK"
     gene            complement(25365..27554)
                     /locus_tag="PKH58_01715"
     CDS             complement(25365..27554)
                     /locus_tag="PKH58_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HNX87773.1"
                     /translation="MKTFRIFILFIVALCGSAQAQLPVLKFNSGKFKIVQFTDLHWVK
                     GSDFQKGNDSTISLMRQIIKLEQPDLVILTGDIVVSGGALEGWKEVTQPMTDAKVPFA
                     ITFGNHDTETDITKKQALEFIQKNHYCMSYNADDEIDGVGNCSLPILSSKGDKNKWVL
                     YLFDSNAYPENKTLGTYNWIQNSQIQWYRKTSDNYTKQNGHNLPSLAFFHIPVPEFGT
                     ASQLPYIVGNKYEDVCSPSINSGLVASFIEKKDILGMFVGHDHNDDFIVAPEGRICFA
                     YGRKTGYNAAYHEVLERGARVINLYENERRFDSYIRTLSNKSLNYTFEQKVDASNYPV
                     AEGTFIQNDLVKNWDDADWQKELKNLRDVGMTYLVFAPTLHTNTDNVTTSIYPTKLAK
                     ETSDSKRDLVDICLRNAQKAGFKVFLGLNFDEKWWTVNFDEKWLIHQMQTGNEVADEL
                     VEKYKAKYPETMYGWYWVWEVANVQQLNSAENKQTLIHSLNVNLDHLNKLTPDMPFML
                     CPFMNHKIGSAKENGELWKSIFEETHFKTGDIFAPQDCVGAGGLELKMVPEWFGEINK
                     AVSSKPGLRFWSDAETFDYRFWNPAPLTRFIKQLKTESPYVSKIITFAYSHYYSPVKV
                     NPCYHNAYLQYVKTGKLPEIKIPSPISKLKITEKNNSVMLKWDAPSEKSTLAGYSVYR
                     NGVQIGNVISDKEGKCKTEFTDKEPSTGKENVYEVKAYNCLGKESQN"
     gene            complement(27568..28257)
                     /locus_tag="PKH58_01720"
     CDS             complement(27568..28257)
                     /locus_tag="PKH58_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNX87774.1"
                     /translation="MKKIIIAICLIQLFSLSIVAQRIKEDFHTKAYISRMDYFSKHPL
                     TTGQIVFLGNSITEAGQWDKYFPEQQPANRGINGDNTQGMLARISEITDAKPNKIFIM
                     AGINDISLGRNNKEILGNYRKIIRSIKKQSPKTQIYIQSVLPINNDFNIYARLKGKES
                     RIVSLNSKLKKLADTQKITFINLYPLYIDKDGKLQKEITADGLHLFPEAYSKWVEKIS
                     TFVTDNNKDTF"
     gene            complement(28289..29368)
                     /locus_tag="PKH58_01725"
     CDS             complement(28289..29368)
                     /locus_tag="PKH58_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HNX87775.1"
                     /translation="MKRRDFLRYSGLASAGLMFSNPLKALGNDNKYKNSENSKIKKIS
                     GSWFEFQHLLPAEGKYWDPDLAKFTADQWREKVKEISEVGFEYLVLQEVAIDGKAIYP
                     SKYAPQYKLGCSDPLEAVLAAGDEFGIKFFLSNDFWDDCQKGDFLMTDPGVKKMRNKT
                     MEEITKKYGHHSCLYGWYFPNESYLMPYFEDKFIKYMNECAHVAKSLMPESVNLIAPY
                     NIKAEKSDDFFVKQLEKMNIEIIAYQDGVGVNSNRVGETAKYFENLSKAHQKASRARI
                     WADMEVFYFEEGTKNNLLPADFNTRILKQMEDISPFVDKILIYQYLGCMNKPNSKAYA
                     GHRNQESVRLYNQYKNWYENEYHPQ"
     gene            complement(29400..31364)
                     /locus_tag="PKH58_01730"
     CDS             complement(29400..31364)
                     /locus_tag="PKH58_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNX87776.1"
                     /translation="MKNKLILFFCFLLPFSVFSKTVYLDELSMKSMLQDWGTPQVNKS
                     VVGTSLKVGGVEFKRGIGTHSISRLLVNLDGKAISFSGFVGADDNNDFSGNIEFRLLA
                     DRKVIWTSNIMRKGMAAKAFDVDLKGVQKLVLLVMEAGDGIMYDHADWLDAKFETNGD
                     ITPLPVVPENIKKDKYILTPKSKDTPQINSPKVFGVRPGSPFLYTVVATGKRPMKFTA
                     YQLPIGLNIDEKTGIITGKIEKAGRYYIVVRADNDLGGDYRKVIIECGDKICLTPPMG
                     WNSWNCWGPQVDEKKVKDAADFMSRELINHGWTYINIDDGWESKTRTKDGVISGNEKF
                     PDFLKLSDYIHSKGLKFGIYSSPGAATCGGYLGSYQYEDIDAQTWAKWGVDFLKYDFC
                     YYRELLSVQTENLIKAPYEKMKEALDNTNRDITYCIGYGAPNVWYWGQEAGGNMWRTT
                     RDITDEWNIVTAIGCFQDVCAFKTKPGNYNDADMLVVGKLGLGWGEKVHETKLSPDEQ
                     YSHVSLWSILSSPLLIGCDMNDMDDFTLNLLTNDEVISVNQDPLVRPANKILTPEGQI
                     WYKELEDGSVAVGLFNIDPCFILWDQNENEKIQNEKHKMTLNFADINLKGRYIVRDLW
                     RQQDLGLFNDSFSTEVAYHGVTFVRLIPEK"
     gene            complement(31390..>32411)
                     /locus_tag="PKH58_01735"
     CDS             complement(31390..>32411)
                     /locus_tag="PKH58_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HNX87777.1"
                     /translation="RDAGYIYINIDDCWHGARDSQGFINADPENFPSGMKALADYVHS
                     KGLKLGIYSDAGRMTCAGKPGSAGHEYQDALTYAKWGIDYLKYDWCYTQDVNPVGAYM
                     LMRDALHNAGRPIVFSLCEWGSSKPWTWAKETGHLWRTTGDITCCFDCPKENGNGVLQ
                     ILDMQEGLREYAGPGHWNDPDMLEVGNGMTVNQDRAHFSMWCMLSAPLLLGNDVRTMN
                     PETKDILMNKEVIAIDQDSLGISAIKIKSENGLEMWFKPLTNDSWAFCILNRTTEPKK
                     YSIHWQQFNFEDKLSKKSTNFYNTIYSIRNLWTKSNEGTTKKDKEVIIPAQDVVMYKL
                     DKIKK"
     assembly_gap    32412..32511
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32512..33849)
                     /locus_tag="PKH58_01740"
     CDS             complement(<32512..33849)
                     /locus_tag="PKH58_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HNX87778.1"
                     /translation="MTYARRKRTIYSQNIIIILGGFLFPFFFSCQSIPKDINEITDNT
                     DTTTKKEQLYVEGTFIQEWLVSDWGDLQWQTEFSALKNVGIKYVILTTALHGDVNNNY
                     TTIYPSSLTFAKQTTTVDVIERCLFNAQMLGIKVFIGINFNDNWWKASTLSNSWLCDQ
                     MEKGNQVAKEIYTNYKSRYNDTLYGWYWVWEIDNLNWQSLVSQNLLIKAMNISLDYLN
                     NLSPDMPVMLSPFVNSVYGTPEQNGGFWKYIFSKTNFKKGDIFAPQDCIGAGGIKIED
                     LARWFKSLRESVNSKPGLQFWSNAENFEQNFWVAAPLQRFVKQTQIVNPYVDKIISFS
                     YTHYYSPNQKTAKFQKTYLEYLKTGILKENAPQSISNLRVVTHDNQFILAWDESSAKE
                     NVAGYYVYENNILIGNFQYNAHEVCTTQLLINPVQDVKKTYSVCSYNSVGTTSS"
     gene            complement(33892..35034)
                     /locus_tag="PKH58_01745"
     CDS             complement(33892..35034)
                     /locus_tag="PKH58_01745"
                     /inference="COORDINATES: protein motif:HMM:NF037325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycan-binding surface protein"
                     /protein_id="HNX87779.1"
                     /translation="MRTIKTINKVMLIVLTLLTIAVTSCKKDNEDLPGMPTFSQVTTI
                     KDLKTPIAGGNMGDWIAIRGTNLQQTDSILFNDVAVNIRDAYYQDSVLYIQVPIKLPM
                     NVNNKITVKTKGGDIAFDFKVNIPDLQLTSIFNEYTPPGDTIKIYGKFIKLYEIDSLN
                     TVVSFGGKESPVIKATDTYITTRVPLDVQSNVKVKAINKKFNVEASCQGYYQDKHGVI
                     TTFDSDYPYISSTGQQWVGDWKKPKPTSGKYLRFEVDQATYPNGLGWFYFFTSDVKYT
                     LDMLQHPDKYTLKFELNMALPVKTTAFFIYYYWAVAPNPMSGETFNVQNLNVWQTVNI
                     PLEKIIPLGYTGTWTDYSLNIRVEDYAPVEQIAMYFDNFRIYKIGD"
     gene            complement(35062..36771)
                     /locus_tag="PKH58_01750"
     CDS             complement(35062..36771)
                     /locus_tag="PKH58_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX87780.1"
                     /translation="MKYNILKYFAGVLILITSFSCSNALDEEVYTFVSGENLIEAKSY
                     NELVSGAFNALTFPFEWGNYHTVVNFDTDYQTGPTWAFGSLGAGNFYENGSNISFYQS
                     YYQCIHRAEYHSFLINQMDIPQDVKDNSKGELAFLTAWSYFNLVQFYGDVPLYKTSVS
                     EGAPLSVPRSPVKDVYAFIIEQLKYAETAMYSTHDAKYVKGHVSRGAAKALLAKVYCT
                     IGSASMAAGNKVYVMEGVPFKFDDDGNKVRISYPSIQSFEKEQVAGYESFNSKEYYKL
                     AMDKAKELINLGDFDLYPSQKELWAPSSKNGKEFIFSLQTLAGNSALSNFVGTDYAGY
                     YDTDGTLSTGYYVQRDHWYQLFDDTDERITWGVIHRVPFNYVKTTGELQYCYYPAKDS
                     VKVRLGIDGYKPTDILRYDAHLYGSKLMKFRQVSVPLDGNRTDFNWPFMRYAEVLLLY
                     AEADNEVNGGPSSEAIAQVDKLNLRNKSKTAAELGTKTPWTQETFRSYILQERVKEFA
                     AEGIRRFDLLRWGIYLQTMNSIGGVDENGVIKRRERKHLLLPLPADEVNTNSFIDTNN
                     PGW"
     gene            complement(36805..>37824)
                     /locus_tag="PKH58_01755"
     CDS             complement(36805..>37824)
                     /locus_tag="PKH58_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HNX87781.1"
                     /translation="TTQYNAGLDISILKNRVNFVIDAYKKYTSDLLQYVYIPISTGFS
                     SIASNYGNVENKGLELAGNFFLIQNKDFTWKMDANISFNRNKIGGLNSDQFSDVCWGM
                     ESMFLRRNGQPIGIIYGYQEDGFFDNEAEVRAYKMYKNETDAKIRSMIGQIKYKDNNG
                     DGVIDPLDKAIIGNTNPDFTYGYTNSFTYKDISLSFFFQGTQGNDILNVNLQNYDLAS
                     TGNMPQFVWDNRWTDANRENAEFPRADNTFTRSLKASERYVEDGAYLRLKNLSLAYRF
                     LHPIKDIEALNLTLSANNLFTLTKYRWYDPDVNTFGSDTSRRGVDMNSYPSARTVSLG
                     IQLTL"
BASE COUNT        10849 a         7982 c         6683 g        12110 t
ORIGIN      
        1 ttagggtttc ttgaatgaag cttatatcgc aacagccact accgaatctg tgtaccagta
       61 gataaagatg tttataaaat aaaccttcac atcccctcat agaaggtgat gtgaggttct
      121 ttttcctgtt ttgattaata taatgttgaa accagtggcg aaactgcttt cacatcatcg
      181 gcatagttgt cggagaaagt gacagcaaaa acagccaccc ggttatcatc cggaaattgc
      241 agttgtgtag ctccttcggg aatttcgagg cagatacgat acatataagt gaaggtataa
      301 gcttcatttc catactttga gctgtgcctg tgactaccga cataagccag tggaacattc
      361 ttaacgaatg atttagtaaa accggtttgt ccccattggc cataaaaacc actgtaataa
      421 ggaatatcgc atttgataac tatatatccg aatttaaaat cagctttaat atcaccatca
      481 gttgaggctg ccaacagata taccttgtta tatttccggt cattaggaat atcaatgata
      541 ttattcttgc acttcacgac attctttgtt tccatatctc caattctgaa aggaattccc
      601 tgactcacaa tctcttttcc gattagctcc gctgcatagg agcaaccatc tttatcaaag
      661 tctccagctt cgttgaaagc gtcgtatgta aaggcattat cagtataagg caattcaacg
      721 ggtgtattgt gaattggctg cgatttcacc gtattccctt ttagctttac agcaaatgtt
      781 ttgggggaaa atgctgttgt tgagaacgtt agtttttttc cgttaaaatc agcttttcca
      841 atctcatgtt ccactccatt tgtttctaca gccgaaacaa tctctgatgg aaattcaacg
      901 gtaacatttt tggcatcttt tccctgggtt tcatagaacc gaagaatgta actttcatcg
      961 ttctgtgctt tcttcagggt ttttaatgcc atttgcgagg tgctgagttt tataaacgag
     1021 aattcacgac ccaatgttcc tttatgacgg ggagtttcaa acgccatcaa cgggttgttc
     1081 aattcttctg atttggaagg gatggacgca aattgaggtt gattttcgtg tccaataatg
     1141 gaataggtaa acgtatgatg tccaaaatcc tgacgggatt ggtaggtgta tctgtcactt
     1201 gttttggggg tatgcagcaa ggtgaggcgc aaggtattat cgcttggttt gtcccatccg
     1261 tatttacagt cattcataat ggatatgccg tatccttcgt cgccggtaat atccgcccat
     1321 tgttgagcac aagtttcata agctttgttc tcattatttc ctcttttgat atagccaagc
     1381 cctaaatcgt aggtagcttt cgggttacta accgacattg ggaattctgc tttcagaagt
     1441 gcgtctgtag ttctccagtc aatctcgttc acaatatcaa ttcgatcatc ggcagctcca
     1501 tctgtcaggc ggatatactg agtaaatttg gaatcgccgt aactgcgttt tacacacaaa
     1561 gaggcgcaag caggtccgtt ttcggcgatg gaaattgaaa caccgtcagt aatcgaaatg
     1621 ggagtagcat caatggtttt tttagatatt tcccatgcag gccattcata agattcgtta
     1681 tttgtgaaaa gagccagcct aaatgttttt cctttctgaa tcaattcttt attgtttcgt
     1741 ttgtcgatca atgaagcaat gtcaccgttg ctgtttaata caattttata aatccggttt
     1801 tccaatgtgt ttgcggaaac cttcaattga ctgacttttt tggctttgcc tgtacgaaca
     1861 tcgtaaagtg catagcctat gggagctaca tccgcgcaaa atacaacttc cgcgtttcca
     1921 tttatatttt taacaagctg ggcattcact tcttttccgt tctgatcgaa aacgcgaaca
     1981 tcctgattgc cttgaggcat actaatttcg gcacgaacta atcctttgtg agccgttgaa
     2041 agcggattat aaacaacaat tggagttcct tttgtattgg tattcatttc tttggctgcc
     2101 ttgcctacgg atgaggtgat tacatcggca aaacaggatt gagaaatcag ttcatcgttc
     2161 caagagaaag tatatgcttt cgggatgctt gttcccgtta aatcgtcgtg aaattgatgc
     2221 cagataaagc gtttccaaga ttcatcaatt tcatcggaag gataagcgct tcctgtaagg
     2281 tagtttgcca gcacggcagc tttttccgat gccactgcca actgctcgtt tctacggttg
     2341 aaacgtttca tagctgcttg cgaagtgtag catccggtac cgtgaatatc catcaacaat
     2401 tcgccatcaa ataccggcaa ttcaggatga tttttaaatg gaagaaattc tttaaaaagt
     2461 tggtcggatg tagcgctaat gatttgtatg tcgccattgc ctttgattcc tttttctacg
     2521 gagattactg acggtatggt tggagaacca cccctgtcgc ctgttccata atatttataa
     2581 gccattttat cgacacttcc atcggctaac tcccgtattt cgttatcgtt gctaatatcc
     2641 tgaccatccc atgaatgaac ataattctgc gaattcagcg aagctattat ttgcgagcca
     2701 tccaagcctt tccatatacc agtataaaac ggcaatttag aatttctcgt atttttataa
     2761 aaaggattgt gcctccattg aagtttttgt gtagaaaagc caatgagtcc gcaatgcgaa
     2821 gcaatggtcg ggagagcata cccaaaaccg aagcaatcag gcagaaaaat atcggtcgat
     2881 tgaattccga actctttccg gtaaaattct tgtcccaaca ggatattccg gaaaaaggat
     2941 tcgggagatg gagtattggg atcggttgca tcccacgagc taccactgac atgccaacga
     3001 ccctgacgaa tatattcctt aactcttttg tatttttcgg gataatactc cttcatccac
     3061 ttgtatttta tgcctccttc gaaattgaaa atataatccg ggtatttttc gaataacaga
     3121 aaattcctgt ccatggtatg gcgcacaaac tggtttatgg acgtctgtac atcccagttc
     3181 cactgagtat cgaaatgtgc attggaaaca aggtaggctt tgtatggctt ctgagcattt
     3241 accattaatc cactgaatag aaaggagagt aatattaatc tggttaatcc ggtacgtttc
     3301 atctgataaa atatttttga cttctttttt ttgtattact gcaataattg taatttctgc
     3361 ttttatcccg atatgctctt ttttacaatg cgtattagag ttcatataag aaccaacatt
     3421 cattactgct ccattcgaat gattcgatta aaaaaattga gatcagcaat gaagttgaca
     3481 aaaaaaaaca gcaacggtta ttgtaatttt cacaagcaat tcattgttga attgcttgtg
     3541 aaaataaagt ttgctttatt tattaaaacc tgtaagagat taattaatta attaagattg
     3601 ttttagtaaa ctcgttttgt cctgctttaa gtcttaccag gtatgcacct ttcgcgaggt
     3661 ttgataaatt gatttccgta tttggtgata cgtttaactg accggcaact tttacacctt
     3721 gtaaattaaa tacagcaaga tcaacatttc cggaggcatt tattctgata attccctttg
     3781 tcggatttgg agatattgcg aatccaagtt ctgatggtgt actaaccgca tctaaatttt
     3841 caggagcgcg aggagccatt tgaactattt cgataatacc cttataatta ccggtaccat
     3901 caaaataatg caaatttaca gtggcaaatt cgggatttat ttccagagtc aacaggtggt
     3961 tcattaaacc attgttgaaa ttggcaggat tatcagtttt attggttcct acgacaatat
     4021 gaggataaat ggttggacct acgatactat caacatccat attgctgcat ccgccctgtg
     4081 tgatatattg tactccatca atcactcctc tttcatatcc gtgcatacga ccactaaaca
     4141 ctgccgtaac accatacttt ttgctcaaca acggaatatt tgttttaaca acttcttgtt
     4201 catacttttg acaacgttcg tagaatggtg cattgtttat aaagataaaa cggaacaacg
     4261 cctttttgga aacatccgat ttcaggaaag tttctaacca tccgtcacga gctaaatcgg
     4321 tattataacg ggttggatca atggaaatga acacagagtt tccatattca taagcaaagc
     4381 taccacttac ttttgaagca tcagaattat atattctagg ttgtactaaa taattgcgta
     4441 tgatgtcagc tcctttcaaa cttgcatcga catccaggtt tcctaaactt tcgataaacg
     4501 gaacttttgt tccaacggtt tccaaaacag cagggataaa cacttttctt aattcaacac
     4561 tatcgtttcc gttaatagtg atattacctg ttgagataca gaaatccggg gctaaattat
     4621 tcactaaata ggtggccata cttgtgaacg gattcaggaa tcgactatca ccccagatac
     4681 caaccgtaaa cactgaatta ggatctttag gagcagtctt aacactttgt atcatatttg
     4741 gtgaactgtt cgaaatagca cggaaaaaat aagtagtacc aggttgcaat ggtttaataa
     4801 ctgctttctg gatatacgta tttgcaccgg atggataagc agtagcaatt atttgagcgc
     4861 cacctgatgc atcagaattt gttccatagt ttactttatt actaccagct tttataaaat
     4921 cgctttccca cattacagtt atagaatccg atgttacatt ttgaacgtaa ggtttgctca
     4981 ggaatgagca aagcgtacga aagtcctgta catatccgcc taaactttga gcttcatttt
     5041 ccgtcagggc tttgttatat agctgtacat ttgcacaatc gatttcgtta tcttcaccag
     5101 aatcgtcgcc aaacagcaaa agtgtaggtt ctaatgaaaa gttcccgtca acaggctgaa
     5161 cataacctgt caaacccttg acaccattta cataaacaga gtattctgaa ccattattaa
     5221 cgacaatgat tgcgcgattc catgtatttg gtcttactgt ctgactggaa tatccggtag
     5281 cacctactcc aacatttcca agcttaccaa gaaacacttc tgcgtcatta ttattcgcag
     5341 gactcgtctg gaagaatgaa taatatttgt ttaattgact taccttaaaa tcgaaaacga
     5401 aagaataggt atttactaat gcatttggga atggagttat accatgaacc agggtaaaat
     5461 tgctacctac acctacacgt acagcgccgt cggtaagcgt ggggccggca actgaagaaa
     5521 ttagtgttcc gtttgtctgt aaatcattac caactgtagc tttaaccaaa ttgccgggat
     5581 tgtcgaactt ccattctcct acaagtccgg aaaccggagt aactacatta aattctacac
     5641 ttgtaactac tgaataacca tcgtctgcca ataaacatac tttgtaaatt ccgggttcag
     5701 taacacctga actaaaaata accgctcccg attcaataag tgtactactg gcatcttgcg
     5761 taccactggt gtaaacccag ccttcattat tcatggaacc agggacagta gtctgatgaa
     5821 atactgccaa ccagtctttt gtagaagttg aaccggtaaa ttttactgat attggttcac
     5881 ctgcaatgta gctatttcta accattgtaa gtttttgtgc ctgaatgtta ccaaacatca
     5941 ggagcaaaaa agaaaagaat aaaagtttct tcataataat taatgtgtta aatttaaact
     6001 agagaattaa attttttaag attaataatg gtaattattt gtttttttga tcagttttat
     6061 tgtctgcgat tgattacgaa agaagatatt taacatataa attccagctg gggcattctc
     6121 aatatcaaaa aaagaagatt gattggctaa caaatccatc tttttattta aaacttcctg
     6181 gccagacagg gatgaaatag aaacagatat tttctcattt tcagaagaaa ttaaagaaaa
     6241 ctgtcctttt gttggattag gaaataccca aatcttagta tcaggtttat ttacggtgct
     6301 gactgatgtt gggacaaatg agaagctgat agtagccgtt actgctttgt gatccgaagg
     6361 ccagggtaaa gtggacgctt caaaaacatc atttccgttg ccttctgtcg tggctgaatt
     6421 cttcacgtaa caagcattgg gtcctacaag cgtagcttca gtggctgtta cacctgtacc
     6481 cttatagaaa atgtaatcaa tccggtcacg ttcgtcgctt aaaggcgccc acgaggtact
     6541 tcctactccg gtggcaacag ctggccaggt tattcccggg ttcaatacct catccggata
     6601 cacctgccga taagcatcaa caaacccatt attttttagc gcgcgtgtag tattccattc
     6661 ataaacaacg ccattatgat catagagatt agcctgtctg gcagtccaat ccagacacga
     6721 cggttcgtta aagtcaccta tcaaaagaac cggacgagtt tcatttttca tataatcgat
     6781 aaaagcagct atctgctcat ctcttcttga gtctaaattc tgagcctgaa tagcagccaa
     6841 atcagtttcc ggaacaaatg gagagaatgc attccagcct gcgtatttac cggatcctcc
     6901 gcaggcatag cctcgtggta aatacgttga ataataagta taatcgaggt gtgaagggca
     6961 tacaactacc gatgctccat tgacattaac cgtaaatggc actgttcttt cgccaacaag
     7021 cggtccggaa gaagttatcg gatatttact gataagcgat acatcagaac ctgttacata
     7081 tcctctgtaa taggtttttc caagcgtagc aagttcattt acaatctttg ttgtccaatc
     7141 tcctgaacta ttggtaactt ctgagaaaca aaccatatcc ggatttacag aattgatcac
     7201 atcacggatt aaagttaaac cgttagctac tgaagttcct tcgcgccaga tattaaattg
     7261 catcactttt aaattcacgg aaagagtctg attagaaact cttataattc cgtttttttg
     7321 tatctgaaga gttccggaag cacctgttgc actgagcgat acatcataaa ctccctctac
     7381 aggataactt accgatggat ttttctcagt actactcgaa ggtgttcctc cggggaaaga
     7441 ccacaaccag tatgtagggg aattcgcaga ctgatcagta aatttcaccg ttccaccggg
     7501 aacaataaaa gtaggagcag cgtcaaatgc tgctaatgat acggaatttg aaatcgtaat
     7561 aatggctgat ttcaggatat tgtatccatc gttggataaa aaacaaactt tatagctacc
     7621 ttctgtactt aatccattct gaaaaacaac ataaccgttg gacaccaaag atgaacccgt
     7681 tttggtacca tctatgtaat accagtcgat ggaaggagat gccggacccg gagtggatgt
     7741 agaaacatac aaaccaaccc aatctttggc actggttgca ccactgaaaa aaacagtaat
     7801 agcctctcca actttataag tggatttatc tgtgctaact gtctgagcag aaagtttcac
     7861 aaccagaaac aagaatataa ggatagaaat tgtgtgtttc atgtgttttt ttatgtctcc
     7921 tgcctgcttg atatctctct atacctctat aataaagggg aaaaggtagg aggaaaatat
     7981 ctgattattt tagcttcacg tcgtttttgc gttctgtttt gtattcaatg ctccccccct
     8041 gccgaatagt ttcccaatca atccagtaag aagtatgttt acgttcgttg atttttactg
     8101 acttaataaa taccggtgac gacgaacctc cggtaatctc gagcattttg cctgacaagt
     8161 ctattttaat attttcaaac tgaggcgtat tgatactaaa tccggctatt ccgggtatca
     8221 tgggataaag tcctattgat gcaaaaacat accaggcccc catcgatcca ccatcgtcgt
     8281 ttccaggcag acctccaggt ttgctggtat acatctgatt aagaatacga taaatcacct
     8341 ctgaagtttt atcgggttta tctgtccagt tataaatcca gggaacctga aaatcaggtt
     8401 catttcctgc agcaaagtaa tggtcatcgt atccggcatt cagtctaaca aacaggctat
     8461 caagcctctt ggaagctgcc tctttaccac ccattgtgtc aattaacgtt ttcaggtcaa
     8521 aagggaccat ccagaaatag ttttccttcg ttgcttcacg ccagtcatca tccggctttt
     8581 tccacgtcat atcatgcgta tctcttgaac gaagccaatt ggtttgcgga tcatagaggt
     8641 ttttccagtt gcccgcacgc ttcataaaat aatcagcatc ttttttattg tttgttgcct
     8701 gcaaagcgaa ctgaccaatg gcaaaatcgg ctgaagcata ttccaggcac aatgaagcat
     8761 tttcaaaacc ttttcggata taattatgca aaccgggtcg tacttctacg ttctgagaat
     8821 aggtaccggc taccatggct ccgcgtttca tatgcttata ggctgtttgt gtatcgaagt
     8881 ccctgcctcc gaaagcataa gtattggaaa ttataatcgg aatcggatct ccttgcataa
     8941 cccctgtttc aatatttgcc atcacccagc ggccataacc tccaccatgt tcggcaaatt
     9001 cgacgaccga ttgcatcata tcggaacttt cttttgggaa aagcatggcc agcaactgac
     9061 actgagtacg gtaagtatcc cagccactgt aggttgtata atagtccctg ccttttaccg
     9121 ttttgtgtgt gttaaaatcc gaaccaatat actctccgtt gcaatcgttg aaaacattgg
     9181 gatgaataat ggaatgatat aaatgcgtat agaattgttt gacacgatcg gaattgcccg
     9241 actgaacctg tattttaccc agacatcggt tccactcttt ttccgcatca tttttcatcg
     9301 tctcaaaacc acgcccttta ttgtccaaac aaaggttctc acgtgcattt tcaattgaga
     9361 cataagaaat agcaatttta tattccaact ctttattgtt ctctgtgtta aaagtaaaat
     9421 atgctccgga gtttcttcca cttattttta agcggtcatc tctcatgtct gagttaatcc
     9481 aggttccacg cagctgaggt tcacggttaa attcagcagc aaaatagact ttgtaatcgg
     9541 tctgatagcc acaaaactca cctccttcgg catagccttc acaggtattc ggagatgtaa
     9601 tcttgatttg tgcgtttgaa acacttgttg agctaattcc tgaacctatt ataaccgttc
     9661 cattgcttgc attatccggg aacttaaacc ggataatgcc gcttcttttt gaaactgtcg
     9721 attcgcatat taccttatct ttcatcgtca ttgagagata tcctgcagta gaattattaa
     9781 tcgtttcata cctgggatag ccattcatgt cattcggcga atcctgaaga tcacctgcca
     9841 tcgctaacac cggaaaatta cccatgttag gacatccggc gcccgatatt tgattaacaa
     9901 caataccttt atgagcagaa aagaaagaag gtgtaagctg catcattcca aacggatatg
     9961 ctgcaccgac aaaggtgaaa ccacatgcaa gaccattacc ttccgttccg atacgtgtat
    10021 ctacgagtcg ggcgtaatta actttctctt cagccttcag taaagaagtg gaaatgaaag
    10081 caaaaattaa tattatatat gagaataatt tcattttatc tgaatatgat agccatgaca
    10141 tgagaatagc tattccatca ttgacggaag ttcctctgtg aaagtctgca gcttttactg
    10201 gataatataa gaaatgccta atgctttaaa attcaactcc ttaatacaga atgcccggtt
    10261 ttttgaattt acaccatcac cgctatagaa accgccttcg ttaccaaaat actgcgtatc
    10321 aggaaccggc actacatcgg gagttagcac gctcattacc ctgaacataa tataacgggg
    10381 tttgaacgag tgatcaactt ccgtgaaaac acacttactg gtttgaggat tggaccgggg
    10441 actggtatat ttaaaccatc cgttacccac ttttttccat ttacgggtgg tggcttctgt
    10501 aacaaaattt gcagcctgac tggatgccat gatataatat ccggtcgttc cgtattttcc
    10561 atcgtcgaga gattctcgtc cccatacttc aaacttcacc acatcccatc ccggataatc
    10621 taaccgacca acgatagaag cacccgaaag atacgcttta gttcctaaat cgaaacagaa
    10681 atgtccggga gcactgatat ttgcacccca gcattcagta tcggcattgt caaataaggc
    10741 agctattccc ttgccgctat aagaatttcc gggatcgtca tttttggtta atgccatagg
    10801 gacaattctt gatttatcaa catcaaagtc aataaccgtt tgttttgcag tttgcgttac
    10861 ttcctgaagc acaagcgtat caacacccaa tttgtcctta ggcagaatat aggtggttac
    10921 tttcagttga aggttttcgt aatcgcccca gttcgggatc attaactttc cacctgttcc
    10981 gtttacatct aacgtttttt cttcggcatt atccccctga taagttaacc ggaacttaac
    11041 agccatcttg gaagtcgaca tggttatctg tgctgtattg ctatcatcga cagtaattgc
    11101 tgtgacagct attttgggtt gaattcttgc atattcttta ccgtatgaag agccgtgaca
    11161 ctccactgtt aatgatttat ttccccaatc gtccacgtta tatatataaa aatcataatc
    11221 tccttccggt aatccctcta taggcatcct ggtataattt cctgttacat agtccttaat
    11281 ctcaaaagtt ttttcgcttg aagccccctg aaaatcgacc cacttcacta tacacttcgt
    11341 agctgtacgg aggtaagtgt tttttgcaac aattatagct cttccattac cacccagagc
    11401 aattactgag tccatccttc ctacgtaact aatttcacca atatctagaa attttcgttg
    11461 agtatcggtg atatcggaac atgcattaaa ggaaaacaca gtgattataa gtccgataaa
    11521 tatctgccaa cttttctttt tcataaaact atattttatc ataacatcat tcttttgaat
    11581 tatttccatt gtgcccagaa agtaaactca gaggcgtaaa ccgagatctg tccatcaaaa
    11641 gcatctatga atttgaagcg aatatacctg atagccggac gggggaacgg agtgtcgatg
    11701 gtgaacataa gtcctttctc tgcagcctga acatcttcag tagttgcggg ttcaccaggt
    11761 ttgcgacccg atggccgaac cacttcaaat gttttatcgt tcagcttggt ccaaccctcc
    11821 agcgggctgg cataattaac tgccggtaat gtagcagcgc cgtacacatc aaaatgtttt
    11881 ggacaggcac cttcaaatac gccgcgttgc tgtaacaaaa tacggccgac aggaactgta
    11941 tcgcctaaat cgatacagag atagtgcgga aattttcctt cgggacctgt tgaagcaaaa
    12001 ttccatatac caactacatc atcccacaac acttgtggcc taccactcca cgaataccag
    12061 tcgtcattat cgaccacttt gggttttccc acatatttaa aattactttt atccaccaat
    12121 tcttccctcc agggtctgtc ttcgaattcc agcgtactgg atgtgttacc gaatctatct
    12181 cgcacaaata caccaaactt gttagttata ttgggtagcc cgactacctg atagacacct
    12241 tcagcctgag taaatacttt agcctgatag aaaacaagtt taccggcaat actgtctttt
    12301 cttaaaaaat aaattgacaa ctcagccttc gatggatttt tatattcaat ccggaatcct
    12361 ccgaaatcaa cattacattt cagactttcg cgcattacct tcacgggcga ttcttccggt
    12421 acaatcgtta tacttaccgg ttctgactgg ttttcacttt tatccataca atataaattc
    12481 acctggtatt ctttcttttc gggaaggccc tccacggtaa gttcggtgtt gtattgcgag
    12541 gaaactacat ttttctttac tccatcaatg taatattcag ctttaacgta taacaaatcc
    12601 ttatcgagcg gcaacacata actgaacttt gctccaccgg gtaatgcttt ataactaaca
    12661 tttgtcaaca ctccgggagc tgtagtatct tctggtccaa aaggtttcaa caagttttct
    12721 tccgtgcagg aaaaaagtgt tgccacaaca gtaagtatta aaattaattt tttcatgttt
    12781 tctttcattt ataagattaa tactaccagc ccatattctg caccagtttg tgatttgaat
    12841 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntccggcaaa ttcagttgct
    12961 tttgagtgaa ttacataact attgtgatcg tcggttattt ccttagaaaa ataccgtccc
    13021 tgatcgaaac ccagataggc ataaaaacgt ggttctcttc cggtgttgta gtaaggcact
    13081 cttgcgtttg tatccgcagt aaaatataaa tgatcctttt ccggtacacg aattaaacta
    13141 aatctatccg agtagtcgta agttttgtct tcttcaattg gaactccgtg ggaggtataa
    13201 aactgatcca ctacgttcat agtggctcca tgaagattgt taaatcctaa cggacgagca
    13261 cctatttgca aagcatacaa cggacaagaa gcgtagtata ttaatcgctg tacatcctga
    13321 cgtcctaccc cccataccag ttctgtattg ttcaagtttt cggtaatctg tccacgcagg
    13381 tttaactcca attgcgttgt atcggtaacc gtcagactat tcattttttg attaaaggta
    13441 tagaaatcca gacctgcatc gtgagctgcc gttattgcag ctttacatgc atctgcagcc
    13501 actttccatt tctcctgaga aaaaacaggg ttcataaatg gcacaccggc cgaattttta
    13561 aagctgctga atttttgatt tccgttaaaa ataggacttg cagcgagtac cagtacctta
    13621 gccttgatag ccaaagcaat cggcaaattg atacgaccta actctgtgcg ccgggcggca
    13681 tatgtaggaa gcaaatcatt gctgttaata gcatcatcca atacagctac tatcttgctt
    13741 acaacatcat ccaccggttc tctttcacgt tgcacctctt cgggagacag tgaaatatcc
    13801 aggttctctc ccataaacgg aataggaccg tacatctgaa gcaaatagaa atgataatag
    13861 gctttcaata ctttcgcttc tgctttccac tgagctcttt cgtcatcagt catatcgggc
    13921 actttatcca cattatcaat gaaggtgtta caatttctga tcccggccca aagactttta
    13981 attttcgttc tctcttccac atcaccaaac ttttggttta ggtttgaata atcacctcca
    14041 tcccaatagt ttaacaatgg atctgttttg tcaagttgtc ctttagcaat gtattcggta
    14101 ctcctgtttc tgtaataata gaagtttggg tccacaaatt caggattcca gttttcatcg
    14161 ccgcacagta atgccggatt ctggaataca tttgctgttc ggggaagata atcgtaacag
    14221 gtatatagca ttttaaatgc attttcacgc gtatcaaaca cttgttgaat actggggata
    14281 ttatccggca caatatccag atagtcggag caggaagcgc aaaggaataa tgaaactgcg
    14341 catattgctg aatatattgt tcttttcata aatctaatta ttaaaagttt aagcttagac
    14401 caagattaaa tactttctgg attggataat ttaatccact tcctcccatt tcaacatccc
    14461 ataacttgaa gttgttaaaa cttagtaaat tcattccact tccatagaaa cgcaattgct
    14521 ggatacccac ttttttgatt aacttttcag gcacattata accgatttcc accgatttca
    14581 ggcgcaaata gcctccatcg cgcagccacc aggtactagc ctgctcgttg tttggccggg
    14641 aggtatctga taaacgtggc cacagagcgt aacaattacg gtttgtttcg gaccaatgat
    14701 cgtcggccca tgctttcaac aacgaatgat tatcaataaa tggagctgtt cccaccgaac
    14761 cgcttcccca gtcaactctc gcagtaggat ccatcatgaa agaaactctt gctgctccct
    14821 gaaggaagaa gttaaaatcg aagccgtaaa aaccaaatga aacaccccaa ccaccttcta
    14881 actgaggaac acctgcatag ccaatgggta ccacatcgtg tgaatcgatg attccatcat
    14941 cattaatatc tttgtatttg atatcacccg gcataaccgg aatgtcattc actttgtcaa
    15001 atgtctgtat cggtgagttg gcaatatctg tttcatcgat aaacaaacgc tctgcaataa
    15061 gtcccttttg ttgtcctatg taggttcctt cggttttcag ccagtccatt ccggcaggaa
    15121 ccatttcttc atagtcagta taacggctca ccgcataggt gaaagtcagg ttgcttctca
    15181 accacacatc tttattgaaa ctatggtttt cggtgagcgt taattcgaat ccggatgatc
    15241 tggctgcccc tacattggca cgcacatcgg cgggtgcttt gccatctgct gccggaaaat
    15301 ccgatagtcc catagttgta ggaattgcgg tacggtttat cagaatacag gttctgtttt
    15361 ctctgaatac gtcgaactga aattccagtt ccttaaccaa tgtaatttct aagcccaggt
    15421 tcatcttagc cgctttctcc caggttatgc ttgtatttgc aaaacggttg aaattaattc
    15481 cagaggagtt tccacctcca gattccccga aatcgttgct aagccctttg ttcatatcaa
    15541 cccgatccag gtagaaaaag cggtcatacg cgtcaccaat agcatcatta cccactaaac
    15601 cataagtcgc ttttaatttc agattagtaa taggactact tttatgccag aatttttcgt
    15661 tggaaataat ccagccgcct ccgaatgagg ggaaaaaacc aaattgatga ttcttagcaa
    15721 aacgctctgc tccgttataa ccaaagttgg attctaaaaa gtatctgtga tcataaccat
    15781 aggtaatacg acctgatatc ccaatgtttc gcgttggtag tgagcgttgc aaatcattgt
    15841 caaaatcaga tgttgtcact tccctcaacg ttcctactgt catggccgag aaattatgca
    15901 tgtcattgat tatcttctca taactggccg ataattctcc gtatattgtt gaattgatac
    15961 ttttattacc tccggcaaaa cctaatgact cgtttccttt cgagaaatta ttcagtttga
    16021 agtatcctgt aatgggatta taggtactca tggtatagta gtaaggaccg taagaacggt
    16081 taatggagta aaccgactga cgggtggtac tagccataaa acgggcagat aatcccgggg
    16141 taacgaaatc aagtttctgt cttaattcta tctgagccac cagattggtt tgtgttgcct
    16201 gctgataacc cttcaccata tctgcgtaag gattgataaa actgtaggat tgtgtacctc
    16261 caaacaaacg gtgtttcgta ttcagattaa tttcatcggg ttgataataa gctgggaaat
    16321 caaccggatt ggagagcata acacgttgat acatctgatt tcctgtgtac ataggaccgc
    16381 ttgactcaag aaaattggca ttaaatttaa tagatcccat ggtggtaggt gtaaaatcaa
    16441 tatcagtatt cgaacgcaga tttacatttt taatactgat gttattgttg aaattattga
    16501 gtttgtccac ctttaatatc ccattatcac tggtcatcgc cgctgcaaca taatacttca
    16561 cccgttgacc accaccactt aagttcatat taaaacgctg attctctgcc ctatccttga
    16621 acagcagatt tagccagtca ttggacggat agtaatacgg attagttccg gcttcaactc
    16681 cggcaatctg atcgtcgctg tacctgcggt tgtactgagg atggcgtgtt acaatggctt
    16741 cattgtttaa tatcatgtaa gtcaacggat cggcaaactc gacagttcgg gtaggctggc
    16801 tggttgattt ttcgtagcgg aagtttaact ttgttttacc ttctacaccc atttttgtgg
    16861 tcaccaggat tacaccgttt gccccgcgtg atccgtaaag cgaagtagcg gttgcatctt
    16921 tcataattga gaaactctga atatcgtcaa ctgacaaccg tgccagatca ttggcggaaa
    16981 gttccatatt gtcaatcagg ataagtggtc ctctcgaata gttgttaaat gtagccacac
    17041 cacgtacgaa aaactgcgca ttatcggctc cgggttcacc ggatgtttgc atggttatta
    17101 aaccggcaat acgtccgcct aaagcagttg tcaggttact ggcaggaaca cgcaggtctg
    17161 ccggtcttac agtggatact gaagctacaa cactttcttt cttctgttta ccaaaagcta
    17221 caattgtaac ttcatccagt gtcttctgtg cttcaaccaa ggttatttta aggtcctcag
    17281 gtcctgttac ctttactgtc ttggtctcat aacccataaa actaacctcc agtacatctc
    17341 caattttagt atttatggtg aaagaaccat ttatatcagt ggcaactccg gtttttggat
    17401 ttgaccgggc tacaactttg gcaccaagaa tagcttctga cttgtcccgt acgacacctt
    17461 ttacttttat attctgcgca gatatctctc ctgaacacaa tgcagaaaac aacataagta
    17521 aaacggcaaa agcatatcgt tttttttgta ttaatttact cattgaaaat tagattgacg
    17581 taactaaaaa ataattaatg tttcattcta gcagatatgc agtttcaact acatattttg
    17641 tgatgagaag gaaaaatctt aagttggctt gtgtttcatg aatttgatat tttattaaga
    17701 ttaaactgat ttattttgta tcgttttttt aatttccgct gaaataaaat tattacgcag
    17761 ctttttccgt tattgagata tatacaaaag caactacgtt ggcaagtcat ataataaact
    17821 tgacacaaag taaaatttac aggattaaaa atggggttaa aatgggatta attaattctg
    17881 taatcttatt aatccacagg ctttttaaac attcagatta tttgagaggg cctgtactac
    17941 attcttaagc gtgaaagcaa ttattcattc taccggatta aattaattcc tgcagattaa
    18001 ataaattctc tgttgctttg acggaataca tttaggtgac cgggtatttt ttgctttacc
    18061 cggcattgta aaatccggtc aggtatgggt aatcgtccac gataaaatac ttttctgttt
    18121 gtgggtgatt agttcaagat ttttaaaagc gttaacacag accggagttc aaagttggga
    18181 aagtatggtg ctcactttta ttccacagcg aatacgtgat agttaataca gggtgtctta
    18241 accgagtaaa tatttgaaca aaatgatact aaactgtttc agcatccatc tgcttcaagt
    18301 ctcaatcaga aaatgtccgg ttgttacttc acttcgaagg aaccggtcaa ccggatatct
    18361 tcggaagaac ttcccagcat tatcgtaaat gtgcctggtt caaccacaaa atgattatcc
    18421 ttgttccaca ggccgagttc ctgaggagtt aactcaaagt taacctcttt ttcttcgccc
    18481 ggtaacaaat gtatgcgttc gaatccacgc aagtttttta cgtaagtggt taccgagcta
    18541 tattcatctc tgatataaag ttgtaccact tcatcacccg gtaatttgcc ggtattttta
    18601 atggtacagc tcagctgatt gttggctaat ggtccgataa cgggatattt aattttcaga
    18661 tttttatatt cgaaagtaga gtaactcaac ccaaatccaa acgggaaaag agggccatcg
    18721 acccgtacat ttcctttcgt gtcggagccc ggtttaaacg ggaacgcaaa aggtatttgt
    18781 cccaccgttt ttggaattgt tacaggcaat ttgccaccgg ggttgtaatc gccgaacaat
    18841 atatgagcca ccgcatcgcc cataaattca cccggaaagc cactgtacag aatagcggga
    18901 atattctttt ttgcccaatt tatagtaact gcacgaccat caatgataag aagaacaacc
    18961 ggaactcccg tcgcaaaaac cgctttcaac aattgctctt gtcggccaca aaggtttaag
    19021 ctggagcgtg aatactcttc acgtactgtt ttttcgtttc cgccaagcac cagaatggca
    19081 acatccgatt ttcgggcttg ttccacagcc tcattaatca tttgttgctc tgctgaatcc
    19141 aacgggacat cgtacaattc actctcaggg aaatgcttat caataatatc acatccttta
    19201 gcataaagaa catccgcatt aggcaaatat gatttaatac cctgatatac tgttttgaca
    19261 ggagcatttg ccggtccgta tcggcaaatc aaattcttag cttcatcggc attcggtccg
    19321 ataaccgcta ttttccgaat gtctttgttt aatggtaaaa tgtttttatc gtttttaagc
    19381 aatatcattg attctaaagc ggctttcatc gaaaccgcct gattggcttt actatgcact
    19441 gttttctctg ataattcttc attgccttta tacggattgt caaaaagttt ttccttgaat
    19501 ttcaccttta acacatccaa aaccctgtca tcaatagttt ttaaagaaac cttgccatca
    19561 acaatggctt gtcttaaagg caagataaaa ttatgcggtc ccgaaaagtt cgtccggata
    19621 tccaatccgg cattaatcac ctgagcaact gcatcttcca ttgtaggtgc aactttatgc
    19681 tttgtgttta aaaattccac tgcttcgcta tccgatacca cgtagccttt aaatccccat
    19741 tcgtttcgaa gaatttgggt gagaaaataa taactgcccg atattggttc accgtcataa
    19801 tcgttgtagg aactcatcac tcccaatgct ccggcttctt gtattgcctt acggaaaggt
    19861 tccaagtaca atgttctcat ttcgcgggga gccacgtgcg gatcggtacg tgtaccttcg
    19921 tcgcgtcccc caaccggtat gctatacacg gcaaaatgct tgggagttga taccaatccg
    19981 ttgctttgca gccctaatac catttgtttc ccaagttcac ccaccagata tggatcctca
    20041 ccgtagcatt ctaccactcg tccccagcgc ggatcctgag ccagatccag aattggcgaa
    20101 taaatattgg taaaacccag tgcattggct tcccgggcgg ttacaccggc tatatctttt
    20161 atcaaagccc tgtcccaact ggctccctga ccgcactgag ccggaaacaa agtggctctg
    20221 tcggcacata aaccccggat tccttcgttg gtaaagtcaa ccggaatacc cagccgggtt
    20281 tcttccacaa accagcgttg gattgcctgc cggttttgta ccccgacagc gtacggaaac
    20341 gaaatagagg aaccaaactt tcccagtcca ttggcctgct catcgatatt accaattcca
    20401 cccttccaga ttgctgtttt ccattcggga gttggcgcaa catcttccat cactctgccg
    20461 gagccataca gcgtagcaag ctgacatgtt ttttcttcca gggtcattct ctgcaaaaga
    20521 tcttcagctc ttgcatcaat ggataacgat ggattttcat agatatccat cactccgttt
    20581 ttattaaaat caatccaccc ttttttatag atattcttat tctgtgtaaa gacagaatgg
    20641 ctgagcagca acactataaa tgtcagttct attttagtct tcatctgttt ttaggacttt
    20701 tccacaaacc ctgtaaaaaa aaggaatgtg aagactgtta tgttattaat tattcatcaa
    20761 aaataaagcc ttcaatgctt gttatttatc tgaaaatgaa tatgggaaac tctcttttgt
    20821 agttccacgt ttgtagttcg gtttattcgt catttggaaa agaatatttc caccttttcg
    20881 caattgagat agtgtaaaat aattcttatt gaattctttg ttatcccaat caattttgtt
    20941 gatataaata ttttcactat tattttcctg agcgttaagc gtaagttttc gattgtcttc
    21001 catggttata acaagttgag aaaaaagtgg tgaacctaag gcgtactcgc ccgaaaccgg
    21061 gcaaaccgga tagaatccca acgccgaaaa tacataccat gccgaagttt gtccgttatc
    21121 ttcatcacca caataaccat cgggtgcagg tctataaagc cggtccataa tctgccgtgt
    21181 ccagtattgt gttttccagg gttcacctgc ccaatcgtac aagtaggtca tgtgctgaat
    21241 aggttgatta ccatgcgcgt actgcccaaa attcattatc tgcatttcgc gaatttcatg
    21301 aattacctgt ccgtaatacg attcatcaaa aaccggagga agcgtgaata ccgaatcgag
    21361 catggcagtg aatttctttc tccctcccat caatgtcgca agtcctgccg gatcatgaaa
    21421 caccgaccat gaataatgcc agctgttgcc ttcggtaaag tctccgcccc acgaaaacgg
    21481 attgaaatca cgccgaaact ctcctttcga attccggcca gccattaaat tatatttcgg
    21541 ataaaaaaga ttcctgtaat tcaacgaccg tttggcaaag gtttcgatag tttttgggtc
    21601 tttgttcaac tccctggcta acttataaat gcaatagtcg gcataggcat actccagcgt
    21661 acgcgctgcg ctttccttta taccgatatc atttggaacg tatccaagtt ccccgtattc
    21721 tttagcaccg gcacgtccca ccgagccaat cgttggatgc gcattaaatg catttttcat
    21781 cacaccttcc cacagcgtat tgatatccac atctttcacc ccgttcagat aagctgaagc
    21841 aaccaccgaa gtggaattgg aaccaaccat aatatcgata tgtcccggag cagcccattc
    21901 gggcagccag ccactttcct tgtaagtatt tgccagacct tccagtatgt atttgctttg
    21961 tgtcgggtaa aacaggttaa agaagggatg aacagctctg aaagtatccc agaaaccgtt
    22021 atcggtgtac atgcgtccgg aacatatttt gccattatac ggactgtaat ggattatatt
    22081 cccttgggca tcgagttcgt gaaactcacg cggaaagagc aaaatccggt acaaacaggt
    22141 atagaaggtt cttatgttat ttaaatcgtt caactgagca ttttccacag agaaacggct
    22201 taaatgactg ttccaaacgg ctttactccc agcacaaact tcatcgaaac tccgaccacc
    22261 cacttctgct ttcagatttt gttcagcttg ttcccagcta ataaaagagg aagaaatccg
    22321 ggcctgtaca gtttctcccc gttgggtcga aaacgtgaca acagccccgc tccgcctccc
    22381 ttccgtctct ttttcattta taagcgtatc ttctttccag acagagttat cgcggattgg
    22441 cttgtcaaaa taaatgacaa aatagttcct gaaattagat ggtacacgct gattttcgta
    22501 cccgatacgt tctgtagaat atcctattac cttattttcc tcagggatta ccttaatata
    22561 agaagccctg gcaaaagcgt caatcaccac atttgcctgt gtggtttccg gaaatgtaaa
    22621 acgtatcaca cacgagcgtt cggttggcgt gagttcggcg gttacgttat aatcggccag
    22681 gtaaactttg taataatgag gtaaggaagt ttctgatttg tgtgagaacc aacttttacg
    22741 gtcgtcttcg cgaaatttct tctcacctac ggtagcaaac agcgaaaaaa caccataatc
    22801 accagtccat ggacttggct gatgtgtctg ccttattccg gtaatgtaat gactgttgta
    22861 ggaatagtac cagcgctcat catttttagt tgctgtttga ggcgaccagt gattcattcc
    22921 ccatggaaga gccgttgccg ggtaaagatt accgtttgat aatgcaaatt cgctctgggt
    22981 tcccatgagc ggcatcacat attgcgtgta atcccgctga gccgacatgc agaaagataa
    23041 taaagtcaag aatgacagta agcagattcg ttgtttcatg gatataatgt tagtttgtta
    23101 atccgtgcct taaaaaaaga agttacaaaa aaaagactta acggttaaaa taacgattaa
    23161 attgcattta agcagcccca caaatacttt aaccggttgc agaaatcagt tttttatcag
    23221 aagaaatcat actgaaggtt gaaaatgata tatcgtcatc tcttttatct ggtgaaaatt
    23281 ttaagattac tattggctgc aaacaggaaa gttgctattt ctggattcaa tttcacattt
    23341 cacttcatcc tgtttctcaa aagcgactga aaaaggtgta gtaggtataa gaaaatatga
    23401 atattcctac tttaacgtga gttctacata agaaaagcgt taaaaaggta tgcatacttc
    23461 agagatagtt gttatcttta taatcctgaa atacaaacaa ataattaaaa cttcatcact
    23521 atattaacca ctaacgaaat tactgaaatt ttttatttat gcgatgaatt ctcaaaagaa
    23581 tttgataaat cacacaaaaa aacatattta acaagagaaa ccgttttact tatcgcaagt
    23641 aaaacggttc aaagaaaact aaatcaacat taatccaatt atttcacctc aaaaaaagaa
    23701 atgcaactat aataatcttg ccaatcaatt acattttttt caaaagcctg aaacaataag
    23761 atttcattat atagtaagct ttttatttgt aaatcaattt gtttggttca tctgaataaa
    23821 taacaacttc tgtaacaatt gcctgtgcat caggctttgc gttcatccag ttgaatgtga
    23881 cagtatgttt tgatttttgc aactgatact tccaaaacaa atccacccga tgcgaattaa
    23941 cagaagcaac aggtaattgt gcagtttcag ctaattttcc atctaaatag acttccaccg
    24001 tagccacata gttggtatcc ggacattgca catatccctt tagcaccact ccaatacctt
    24061 caaaatcaat ttttggttta tctttaatac ttttatttac tgatattttt tttaccggat
    24121 aatgattttc aaacgatttt tcatatttta cggcttttgg tttttgacgg cgaatagaaa
    24181 cctgttcctt ttcaactttt cctccctgtt gggctatcac ttgcagggcg tgtttcattc
    24241 caatatcata aatttttgtt aaggaaagat cagtatagat aaaattaata tctttcacct
    24301 gatctaaact ttttcgccaa tactcgggta tatttttgta gccaaccatg gtaccgagaa
    24361 cacctgctgc tgtagccgga ttgcaatctg catcttgtcc gcaacgggct gcgatatcta
    24421 tggttttgaa gaaatctttt tctccgtaca acaaaccgat aacgacgtat gcagaattaa
    24481 gtttcgcatc tatatcaaaa ggagtgaaaa ctccatcggg gcatccgttc tcagcactcc
    24541 attttttttc acactcgaac caggtacgtt tccaatccga gggataagct gtgtgccatt
    24601 taataatatc agaaatacac tgataaaagc tactctgctg tggaatggtt ttcaaggctt
    24661 ctgaaactac gaaattgata tcggtagaag taaaagccat tgaatacaac gcacccacaa
    24721 atacgcctcc ataccatccg tcgccataac aaatcagatg tccgacttta tcagaaattt
    24781 cgctggcgga atttggcatt ccgggtgaca taaggcctgc aaaatctgcc tcaatctgat
    24841 aatcgatatc atctgcatga ggattattga gccaatttcc ggcttctgat ggttttactc
    24901 cgtgctgtaa attgtagcgt gcaacctgat tggcatgcca tagcggatag ggtgcgtgag
    24961 caaaagctgt ggcaaaatca gcgggctgtg cattcaaacc catcttttca aatacttcta
    25021 caaatgtgag gttgacatag atatcatcat acagtcccgg aaaattatca aaataccatt
    25081 ttacgcgatt atcatcccac ggtattggca cataatcctg aatcatggta ccgttatatt
    25141 gaaactcaac cggaccgcca tacgtgcatc caatcgtttt tgcagcccaa ccgcccttaa
    25201 ttttatcaaa aaggtcagtt ttagttattg ttaggatttt tttttctgga ttattttttg
    25261 cttgaccggc agcaaaactg agtgctcctg cagaaattgt catcagaaaa cttaatatta
    25321 aggtgatttt tctcatatct tatttttttt gaatttcttt atgtttaatt ttgggattcc
    25381 tttcccaagc aattatatgc tttcacttca tatacatttt cttttccagt agatggttct
    25441 ttatccgtaa attctgtttt acactttcct tctttatcag aaataacgtt tcctatctga
    25501 actccattcc gataaactga ataaccggca agagttgatt tctcggaagg agcatcccat
    25561 tttagcatta ccgaattatt tttttccgta attttgagtt tggaaattgg agaaggtatt
    25621 tttatctccg gcaatttacc tgtttttacg tattgcagat aagcattgtg atagcacgga
    25681 tttactttaa caggactata ataatggctg tatgcaaatg tgatgatttt acttacataa
    25741 gggctttctg tcttcagttg ttttataaat cgtgtcaacg gagccggatt ccagaaacga
    25801 taatcaaagg tctctgcatc agaccaaaac ctcaatcctg gtttggagct gactgctttg
    25861 ttaatttcac caaaccactc aggcaccatc ttcagttcga gtccaccagc tcctacacaa
    25921 tcttgtggag caaatatatc acctgttttg aagtgtgttt cctcaaaaat tgatttccat
    25981 aactctccat tttcttttgc tgagccaatt ttatgattca taaacggaca aagcataaaa
    26041 ggcatatcag gtgtcagttt attcaaatga tccaaattga cattcagtga atgaatcaaa
    26101 gtctgtttgt tctcagctga attcagttgt tgaacattgg ctacttccca aacccaatac
    26161 catccataca tagtttctgg atattttgct ttgtattttt caactaattc atcagctact
    26221 tcattacctg tttgcatctg atgtattaac catttttcat cgaaattcac cgtccaccat
    26281 ttttcatcaa agttaagccc caaaaagact ttaaatcctg ctttctgagc attccgaagg
    26341 caaatatcta ctaaatctcg tttcgaatcg ctcgtttctt ttgccaattt tgtgggataa
    26401 atacttgttg ttacattatc agtattggta tgcagtgtgg gggcaaaaac cagatacgtc
    26461 atcccaacat ctctcaggtt tttcagttct ttctgccagt cggcatcatc ccaatttttc
    26521 acaaggtcat tctggataaa ggtaccctct gctaccggat aattactggc atctactttt
    26581 tgttcaaatg tgtaattcaa agatttattt gataacgtgc gaatatacga atcaaaacgc
    26641 ctttcattct cataaagatt aatcacacgc gctcctcttt ctaaaacttc gtgataggct
    26701 gcattatatc cggtttttcg tccgtaggca aaacatattc tcccttcggg tgcaacaata
    26761 aaatcatcgt tgtgatcatg tcctacaaac attccaagga tatctttctt ttcaataaac
    26821 gaagcgacca atccgctgtt gattgacgga gagcacacgt cttcgtattt attccctacg
    26881 atatatggaa gctggcttgc cgtaccaaat tcaggaacag gaatatgaaa aaatgccagt
    26941 gacggtaaat tatgtccgtt ctgttttgta taattgtcac ttgttttccg ataccattgg
    27001 atttggctgt tttgtatcca gttataagtt cccaatgttt tattttcggg atatgcattt
    27061 gagtcaaata gatacaatac ccatttattt ttatcacctt tggatgataa aataggaagt
    27121 gaacagtttc cgacaccgtc aatctcgtcg tcggcattat atgacatgca atagtgattc
    27181 ttctgaataa actccaacgc ctgttttttt gttatgtcag tttctgtatc gtgattacca
    27241 aacgtaatcg caaaaggaac tttagcatca gtcatgggct gtgtaacctc tttccagcct
    27301 tccaatgcac ctccggatac cacaatatcc cccgtcaata tcactaaatc tggttgttct
    27361 aatttaataa tttgacgcat taacgaaatt gtactatcgt tgcctttttg gaaatctgaa
    27421 cctttgaccc aatgcaaatc tgtaaactga acaattttaa atttaccgct attaaatttt
    27481 aatacaggca attgagcctg tgcactacca cataaagcta ctataaaaag aataaaaatt
    27541 cggaaagtct tcatgaatta ctaattttta aaaagtatct ttattgttat cagtcacaaa
    27601 agtgctaatt ttttctaccc atttactgta tgcttcggga aataaatgaa gtccatcggc
    27661 tgtaatttct ttttgaagct ttccatcctt atcaatataa agagggtaaa gattaataaa
    27721 ggtaattttc tgtgtatcag caagtttttt caactttgag ttgagtgata caatcctact
    27781 ctcttttcct ttcaaacggg catagatatt aaaatcgttg ttgattggca acacactttg
    27841 aatatatatc tgcgttttag gagattgctt tttaatggat ctgataattt tccggtagtt
    27901 tcctaagatt tccttgttat tgcgcccaag tgaaatgtca tttatacccg ccataatgaa
    27961 aatcttgttt ggttttgcat cggtaatttc tgagatacga gccaacattc cctgtgtatt
    28021 gtcaccgttt atgcctctgt ttgccggttg ttgttcaggg aaatatttat cccattgtcc
    28081 ggcttcggtt attgagtttc ccagaaacac gatttgtcct gtggtcaagg gatgtttcga
    28141 gaaataatcc attcggctta tatatgcttt tgtatgaaaa tcttctttga tacgttgtgc
    28201 tacaatcgat aacgagaaaa gctgaataag gcaaattgca attataattt tcttcatttt
    28261 tttatttttt ttgaatgttt aatgcagttt attgcggatg gtattcgttc tcataccagt
    28321 ttttatactg attatataat cgaacagatt cctgatttcg gtgtccggca taagctttcg
    28381 aattaggttt attcatacat ccgaggtatt gatagatgag aattttgtca acaaaaggtg
    28441 atatatcttc catttgtttc aaaattcgcg tattaaaatc ggcaggcagc aaattgtttt
    28501 ttgttccttc ttcgaagtag aagacttcca tatcagccca aattcttgct cttgatgcct
    28561 tttgatgtgc tttggataga ttttcgaaat atttagccgt ttcacccact ctgttggagt
    28621 taacacccac accatcctga taagcaatga tttcgatatt cattttctcc aattgtttaa
    28681 caaaaaaatc atccgatttt tcagctttaa tattataggg cgcgattaga ttgacagact
    28741 ctggcattaa tgattttgcc acatgagcac attcattcat atattttata aacttatcct
    28801 caaaataggg catcaaatac gattcattgg ggaaatacca gccgtaaaga caagaatgat
    28861 gaccatattt ttttgtgatc tcttccatgg ttttgtttct catttttttt actcccgggt
    28921 cggtcatcag gaaatcacct ttctggcaat catcccaaaa atcattgctc agaaaaaact
    28981 taattccgaa ctcatctcct gctgccaata cagcttccag cgggtctgaa catcccaatt
    29041 tatattgtgg tgcatatttt gatggataaa tagctttccc atcgatggct acttcctgca
    29101 atacaaggta ttcaaagcct acctcactta tttcttttac tttttcacgc cattgatctg
    29161 ctgtgaattt tgccaaatca ggatcccagt atttaccttc agccggaagt aaatgttgaa
    29221 attcaaacca ggagccggaa atcttcttta tttttgagtt ttcactattt ttatacttat
    29281 tatcattacc taacgctttt aaaggatttg aaaacatcaa tcctgctgaa gctagtcctg
    29341 aatatcttaa aaaatctctt cttttcattc gaatatgaat tattattttg tatttctatt
    29401 tatttttcag gtatcaacct aacaaaagtt actccatgat aggctacttc tgttgaaaat
    29461 gaatcattaa ataatccaag atcttgttgt ctccataaat cgcgcacgat atatcttccc
    29521 tttaggttta tgtcagcaaa attcaatgtc attttgtgct tttcgttttg aatcttttca
    29581 ttttcgtttt gatcccataa tataaaacaa ggatcaatat taaaaagacc taccgccaca
    29641 gagccgtctt ccagttcttt gtaccaaatc tgaccttcgg gtgtaagaat cttgtttgca
    29701 ggtctgacca acggatcctg atttacactg atgacttcat cgtttgttaa aagatttagt
    29761 gtaaaatcat ccatatcgtt catatcacac ccaatcagta acggacttga taaaatactc
    29821 caaagactta cgtgggaata ctgttcgtcg ggcgaaagtt ttgtttcatg tactttttct
    29881 ccccaaccca aaccaagttt tccaactacc aacatgtcag catcattata gttgccgggt
    29941 tttgttttaa atgcacatac atcttgaaaa caaccgatag ctgtaacgat attccattca
    30001 tccgtaatat ctcgtgttgt acgccacata ttaccaccag cttcttgtcc ccaataccat
    30061 acgttaggag caccatagcc aatacaatag gtgatatccc ggttagtatt gtccaatgct
    30121 tctttcattt tttcatacgg agccttaata aggttctctg tttgaacaga aagaagttcg
    30181 cgatagtaac agaaatcata tttcaggaaa tcaacacccc atttcgccca tgtttgagca
    30241 tcaatgtctt catattgata tgaccccaga taaccaccgc aagtggcagc accaggtgat
    30301 gaatagatgc cgaatttcaa tccttttgaa tgaatataat cacttaattt cagaaaatca
    30361 gggaacttct cattcccact tatcacacca tctttcgttc ttgtctttga ttcccatcca
    30421 tcatcgatgt tgatatacgt ccaaccatga tttattaatt ccctgctcat gaagtcggct
    30481 gcatctttta cttttttttc atctacctga ggtccccagc agttccagct attccatccc
    30541 atgggaggag taagacatat tttatcacca cattcgataa tcactttgcg gtaatcacct
    30601 cccaaatcat tgtctgctcg aactacaata taatatcttc ctgctttttc aatttttcct
    30661 gttatgatac cggttttttc atcaatgttc agacctatcg gcaactggta tgctgtaaat
    30721 ttcattggtc tttttcctgt tgcaactaca gtatataaga aagggcttcc cggacgtacc
    30781 ccaaaaactt tcggtgaatt aatttgtggg gtatcttttg atttgggagt aagtatatac
    30841 ttgtctttct taatattttc aggaactaca ggtaaaggag ttatatcccc attggtttca
    30901 aattttgcgt ctaaccaatc ggcatggtca tacataatac catcgcctgc ttccattact
    30961 aacaatacca atttctgaac tcctttcaaa tctacatcga atgcttttgc cgccattcct
    31021 ttccgcataa tattgctcgt ccagattact ttccggtcgg ctaacagtcg aaactctatg
    31081 tttcccgaaa aatcgttgtt atcatcggca cctacaaaac cggaaaaaga aatagcttta
    31141 ccgtccagat tgactaataa acgactaata gaatgtgttc caattcctct tttgaattcg
    31201 acgcctccta cctttaatga tgttccaaca acggatttgt ttacctgcgg cgtcccccag
    31261 tcctgaagca tagatttcat actcagttcg tccaggtaaa cagttttgga gaaaactgaa
    31321 aagggaagca agaaacaaaa aaacaggatc aatttgtttt tcattttttt tatattgatt
    31381 atcagtattt tattttttaa ttttatctaa tttatacatc acaacatctt gtgccgggat
    31441 aattacttct ttgtcttttt ttgttgttcc ttcgttggat tttgtccaaa gatttctgat
    31501 agaatatatt gtattgtaga agttggttga tttctttgat agtttatctt caaaattaaa
    31561 ctgctgccaa tgaatagaat attttttggg ttctgtggtt ctgttgagaa tgcaaaacgc
    31621 ccaactgtca tttgttaatg gtttaaacca catttccagc ccattttcgg attttatttt
    31681 tattgctgat attcctaacg aatcctggtc gatagcaatt acttctttgt tcatcagaat
    31741 atcttttgtc tccgggttca tggttcgcac atcatttccc aacagtaatg gagctgacaa
    31801 catacaccac attgaaaaat gtgccctgtc ctgattaaca gtcattccat ttcccacttc
    31861 cagcatatca gggtcattcc aatgtccggg cccggcatac tctctaagtc cctcctgcat
    31921 atccaatatc tgtaatactc cattaccatt ttccttagga cagtcgaaac agcaggtgat
    31981 atccccggtg gtacgccata aatgtcccgt ttcttttgcc caagtccagg gtttacttga
    32041 gccccattca cacagactga aaactatagg tcttccggca ttatgaagcg catcacgcat
    32101 cagcatataa gctccaaccg gattaacgtc ctgcgtataa caccaatcat atttcagata
    32161 gtcgataccc cattttgcat aggtgagagc atcctgatat tcatgccccg cagagcctgg
    32221 tttgccggcg caggtcattc ttccggcatc cgaataaatg cctaatttca accctttgga
    32281 atgcacataa tcggctaatg ctttcatacc cgaaggaaaa ttttcgggat cagcgttgat
    32341 aaatccttga ctatctctgg caccatgcca gcaatcatca atatttatat aaatgtaccc
    32401 tgcatctcgc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgatgatgt
    32521 tgttcctacc gaattgtatg aacaaacgga atatgttttc tttacatcct gtacggggtt
    32581 aatcaaaagt tgtgtagtac aaacttcgtg agcattgtac tgaaaattgc caatcaaaat
    32641 attgttttcg tacacataat atcctgctac attttctttg gctgaagatt cgtcccaagc
    32701 aagtataaac tgattatcgt gtgttaccac tctcagatta ctgatacttt gcggggcatt
    32761 ttctttaaga atgcctgttt taagatactc aagatatgtt ttttgaaact tcgctgtctt
    32821 ctggttaggg ctgtaataat gtgtatagga aaatgaaatt attttatcaa catagggatt
    32881 aacaatctga gtttgtttta caaatcgttg caaaggtgct gctacccaaa aattttgttc
    32941 aaaattttca gcattagacc agaattgcag tccgggtttt gagttaacac tttctctaag
    33001 tgatttaaac catcttgcta aatcttcaat ttttattcct cctgcaccga tacaatcctg
    33061 cggagcaaat atatctccct ttttaaagtt tgttttcgaa aaaatatatt tccagaagcc
    33121 cccgttctgt tccggagttc cataaacaga attgacgaaa gggctaagca ttaccggcat
    33181 atcaggacta agattattta aataatccag actgatattc attgctttaa ttaataaatt
    33241 ctgcgaaaca agtgattgcc agttcagatt atcaatttcc caaacccaat accaaccata
    33301 caacgtatca ttataccttg atttatagtt ggtatatatt tcttttgcta cctgattgcc
    33361 tttctccatt tgatcacata accaggaatt tgataatgtc gatgctttcc accagttatc
    33421 attaaaattt atgccgataa atacttttat ccctagcatt tgagcattga acagacacct
    33481 ttcaatgaca tcgaccgtag ttgtttgttt tgcaaatgta agtgaagaag gataaattgt
    33541 agtataatta ttattcacat ctccatgcaa cgccgttgtc aaaattacat atttaattcc
    33601 aacgtttttt aatgccgaaa attctgtctg ccattgtaaa tcgccccaat cggacaccaa
    33661 ccattcctga ataaatgttc cttcaacata cagttgctct tttttagtgg tagtgtctgt
    33721 attatctgtt atctcattaa tatctttcgg gattgactga catgagaaaa agaaaggaaa
    33781 aagaaaacca cccagaataa taattatatt ctgcgaataa atagttctct ttctccttgc
    33841 gtaagtcata acgtaattac agctatttca tataaatttt cccgggaaaa tttaatcacc
    33901 tattttatat attctaaaat tatcaaaata catggctatc tgttctaccg gagcataatc
    33961 ttctacacgt atattcagag aataatctgt ccaagtaccg gtatatccca gtggtattat
    34021 tttttctaac gggatattaa ccgtttgcca cacattaaga ttttgcacat taaatgtttc
    34081 tccgctcata gggttgggcg caacagccca gtagtaataa atgaagaaag cagtagtttt
    34141 tacaggcaaa gccatattga gctcaaattt taatgtgtat ttatcgggat gctgcaacat
    34201 atccaatgtg tattttacat cgcttgtaaa gaaataaaac catcccaacc cattgggata
    34261 cgtagcttga tccacttcaa aacgaaggta tttacctgat gttggttttg gttttttcca
    34321 atctccaacc cattgctgac cagtagaact tatgtacgga taatccgagt caaaagttgt
    34381 aatcacaccg tgcttgtcct gataataacc ctgacaagat gcttcaacat taaatttttt
    34441 attaattgcc ttcactttta catttgactg aacatctaaa ggtactctgg tggtaatata
    34501 agtgtctgtt gcttttatca ccggactttc tttgccccca aatgagacta ccgtattcaa
    34561 actatcaatt tcgtagagct ttataaactt accataaatt tttattgtat ctccaggagg
    34621 tgtatattca ttgaaaatgc ttgttaactg aagatccggt atgttaactt tgaaatcgaa
    34681 agcaatatct ccacctttcg tttttacagt aattttatta ttgacattca ttggtaattt
    34741 tattggtacc tgaatgtaca acacactatc ttgataataa gcgtctctga tattcacagc
    34801 aacatcgtta aataaaatag aatctgtctg ttgtagattt gtacctctaa ttgcaatcca
    34861 gtctcccatg tttccacctg caattggtgt tttcaggtct tttattgtag taacctgact
    34921 aaaagtaggc atacccggca gatcttcatt atctttttta caagatgtga ctgcaatggt
    34981 aagcaatgtt agtactatta acattacctt atttattgtt ttaattgttc tcatgcgtaa
    35041 actttttttg attattaata attaccatcc agggttgttt gtatcaataa atgagtttgt
    35101 atttacttca tcagccggaa gaggtaataa aaggtgcttt ctttcacgtc ttttaataac
    35161 tccgttttca tcgactccgc caatagaatt catcgtttga agataaatac cccaacgcaa
    35221 taaatcaaac cggcgaatgc cttctgctgc aaattccttt actctttcct gcaggatgta
    35281 ggatctgaat gtttcttgcg tccagggtgt ttttgttcct aactctgctg ctgttttgct
    35341 tttattccgc agatttaatt tatctacctg agcaatagcc tcacttgaag gacctccatt
    35401 tacttcattg tcagcttcag cgtacagcaa taatacttca gcataacgca taaatggcca
    35461 gttaaagtct gttctgttgc catccaaagg aacagagact tgtctgaatt tcatcagctt
    35521 agacccatat aaatgtgcat catatcttaa aatatcagtt ggtttatatc catcaatgcc
    35581 taacctcact ttaacactgt cttttgccgg ataataacaa tactgaagtt caccggtagt
    35641 cttcacataa ttaaatggaa cacgatgtat tacaccccat gttatccttt catctgtatc
    35701 gtcaaaaagc tgataccaat gatcacgctg cacataatag ccggtgctga gggttccgtc
    35761 agtgtcatag tatccagcat aatctgttcc tacgaagttg ctcaatgccg aatttccggc
    35821 caaggtttgt agtgaaaaaa tgaattcttt accattttta ctggacggtg cccacagttc
    35881 tttttgggag ggataaagat caaaatcccc taagttaata agttctttgg ctttgtccat
    35941 agccagtttg taatattcct ttgaattaaa agactcataa cctgctactt gttccttttc
    36001 gaatgattga atggatggat atgatatcct cactttattt ccatcatcgt caaatttaaa
    36061 tggaactcct tccattacat atactttatt ccctgccgcc atagatgcag agccaattgt
    36121 acaatagacc tttgccaata atgccttagc agcgccacgc gacacatgcc cttttacata
    36181 ttttgcatcg tgtgtggaat acatagctgt ttctgcatat ttcaattgtt cgataataaa
    36241 agcatacaca tctttcacag gtgaacgcgg aacagacaaa ggcgctcctt ctgatactga
    36301 tgttttatac aaaggaacat ctccataaaa ctgtaccaaa ttgaaatatg accatgctgt
    36361 cagaaaagct aattctcctt ttgaattatc ttttacatcc tgaggtatat ccatttggtt
    36421 gattaagaaa gaatggtatt cagcccgatg aatacattga taatatgatt gatagaagct
    36481 aatatttgaa ccgttttcat agaaattgcc ggctcccaat gatccgaaag cccaagttgg
    36541 tccggtttga taatctgtat caaaattgac gacagtatga taattacccc attcgaaagg
    36601 gaaagtcaac gcattaaatg cgccggagac taattcattg tagctttttg cttcaatcaa
    36661 gttctcacct gatacaaaag tatatacttc ttcatcaagt gcattggaac aggaaaaact
    36721 cgtaatgagt attaagactc ctgcaaaata ttttagaata ttatatttca tttttttaaa
    36781 ttattttgaa tgaatacttg tgaattataa tgttagctga attcccaatg aaactgttct
    36841 tgcacttgga tatgaattca tatctacacc tctacgggat gtgtcactcc caaacgtgtt
    36901 tacatcaggg tcataccacc ggtattttgt aagtgtgaaa agattgttgg ctgatagtgt
    36961 tagatttaaa gcttcaatat cctttatcgg atgcaaaaac cgatatgcaa ggctcaagtt
    37021 tttcaatctc aaataggcgc catcttctac atatctttca gaggctttca aacttcgagt
    37081 aaaggtatta tcagcacgcg gaaactctgc attttctctg tttgcgtctg tccatctatt
    37141 gtcccataca aattgtggca tattgcctgt agaagctaga tcgtaatttt gtaaattaac
    37201 attcaggata tcattacctt gtgtcccctg aaagaaaaag ctaagtgaaa tatctttata
    37261 agtaaacgag ttggtatatc cataagtgaa atcgggattt gtatttccaa taattgcttt
    37321 atctaaagga tcaatcacgc catcaccatt attatcttta tatttaatct gaccaatcat
    37381 acttcttatc ttagcatctg tttcattttt atacattttg tatgctctca cttcagcctc
    37441 gttgtcgaaa aatccatctt cctgatatcc ataaataatg cctatgggtt gtccgtttct
    37501 tcggaggaac atactttcca ttccccagca cacatcagag aattggtctg aatttaaccc
    37561 gccgatttta ttccggttaa aagatatgtt tgcatccatt ttccaggtaa aatccttatt
    37621 ttgaattaaa aagaaattac ctgctaattc aagacctttg ttttcgacat tcccatagtt
    37681 ggatgctata gaagaaaaac ctgtgcttat aggaatatat acgtattgaa gcaaatcgga
    37741 tgtatatttt ttataagcat ctatcacaaa atttactcga tttttcagaa tgcttatatc
    37801 aagacctgcg ttatactggg tcgt
//
LOCUS       DAOPYB010000011        37762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_979_length_37762_cov_8.867213, whole genome shotgun sequence.
ACCESSION   DAOPYB010000011 DAOPYB010000000
VERSION     DAOPYB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 37762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37762
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_979_length_37762_cov_8.867213"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2062)
                     /locus_tag="PKH58_01760"
     CDS             complement(<1..2062)
                     /locus_tag="PKH58_01760"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNX87782.1"
                     /translation="MKASKFIIILAGLLLFINPAQGQDLSGVRIFINPGHGGYDSDDR
                     NVVIAPFTSGDQNGFWESKSNLDKGFTLRSMLLTHNADAMMSRTKNTTSDDLPLSAIV
                     QMANEYNADFMLSIHSNAGAGSANHVLMLYSGVDAGDTHVYPTPTPYSELSKAISTEI
                     AKNLYSNQITAWSSSYSVRGDKTFGRVAMDNWSDGYGVLRGLTVPGVISEGSMHDYIP
                     ETYRLMNMDYKRLEAWHFLKSFATYFKTAALSTGNIAGSVRDKYLLNEASYYKLANSK
                     DLYLPVNGATVTIIPGDSVCTTDNLNNGVFSFENLPAGTYSLITRHPAYHPDTTQITV
                     EANKTSYASVRMNKIRNTPPQVVEYSPNVTLTDSVSSGSTIWMRFNWDVDKTSAIEAF
                     SITPHIDGDIVFRDANYLMEFIPRVPLDKSTIYTVRLAKTVRHPDNLYMENDFVFQFR
                     TRSRNELKLLASYPMPDETHVDYKWPVFTFVFDKKLQTTELIDGVQVYDKSGKKITKN
                     LRSLRHNTVQTPYGSTSFIVGQDLTPGQEYVVKLAKGITDIDGVYLTDTLKIPFTASD
                     ERVTDRMVAENFETAGKISVDETQGRNVTSASVQSSSSTRLFDTYSYNLKYTFSDKTG
                     GEVVYKLNTPTLNVNRDSVMGLHLYGDLSGNELYLMFTSGNDMRFIHADSIHYGFWKY
                     VEVPL"
     gene            complement(2062..2943)
                     /locus_tag="PKH58_01765"
     CDS             complement(2062..2943)
                     /locus_tag="PKH58_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87783.1"
                     /translation="MKNKQTVMLLFVCLMAYSNMSGQLVKITKKEKVGIEKSVFYPVL
                     NEKGDKLLYTDVNYKGLKLYDLTNNKLTPISDADGAGFEPLFESKTGNILHRETRFVD
                     RRKYQSVQRYNPVNKQRSVLIQSTRNLKSTLPPAARTAARTETDIRVYSNAQSVIVSV
                     NGIEKELKPIDETAVSGYIWASLSPDKKRMLFYATGKGTYIADLNGNPISYLGNLQAP
                     RWYNDNQVVGMDARNNEYVYTSSRILMVAADGKTKQYLTDDNEMAMYPSASAVSGKIA
                     YNTYEGDIFILTVNPVK"
     gene            complement(2946..5453)
                     /locus_tag="PKH58_01770"
     CDS             complement(2946..5453)
                     /locus_tag="PKH58_01770"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNX87784.1"
                     /translation="MKSLRLHIILLVSVFLINSINLSAGKIEILGVSKNIDTLEHKSV
                     GPGIQYTKFVLPDYPLSGYMLVVDLTNPYNQVETFQAQNQVGKTEAMSSAYSRLDREQ
                     HRTIAGVNGNFWIVSGQGQPDALLGVPHSGSAFNGEMITDPNNWNRGHGSIGFAMIDS
                     DKKAWIDDISFVGTVKIPGKGEYPVSQINRLRNINELVLFNNFLGSKVTQTDDDGIEV
                     FIKPVTGKKWEVNSDVECVVTRIVSNKGGNSLMEGETVLSGNGTARTFLNNLSAGDKL
                     FVNMAVKTMTDNQFPSVAQMVTGNALVMKDGTLTNRNYNEAYNTQLYPRTGIGSSMDG
                     KTLYLIVIDKGGKSAGASTETMCGILKASGAQHATSMDGGGSAQMMLDGQIVNNPSDG
                     KERPVANGWFLFHNAQEDNVITRIEFANHQIRIPSYSSFTPVVLGYNRFGVLVNKNVT
                     GFTLSCSPEIGTISQNKTLVASGNPGTGSLIASFNGVTATKPIEVVSGQVSLKCDSIL
                     MDTRNDYPIEVISNSTGTLMSVSPEFLSWEVKDPAICEVRNGILKGLKNGKTFVYGSL
                     GEFKDTLLVRVEIPAAPSMIADSIHTADWAMTASTFLNAQWNTESLPQGWKTGSVVNF
                     VYAAGRAPSIKLTNKSTLYGLPDTVKLVINIGDIALTKAIVSLQANNDIKLVSRDFTA
                     LEQNKDISLNIPVDQLMDVNNRAIYPVKFDNVNFYIDAAGMTAQKSYSLAVKEVSLSF
                     KDFVISYTAPVKMNMFKVYPNPAKDGISYIYLKLDRPEDVRIDVVGQNGQILRSKKLT
                     NMQSGEVKLPLDGLNKGMYLVNVIRKQETESVKILLK"
     gene            complement(5457..7901)
                     /locus_tag="PKH58_01775"
     CDS             complement(5457..7901)
                     /locus_tag="PKH58_01775"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNX87785.1"
                     /translation="MRKLYTSFLFLFILSGVFAGNIVIDGKSYTIDTVANFKVGPGSY
                     YTAVELRGYKRLNIFFLKVDLNNPYITYRTALGRDSIYGTERPTTTATRKSKEGAVYF
                     AGTNGDFYDTGSTYNGMPTGASMFEGEVGTGPISTPVMSFDSNKVPYIGTLTLTGRVT
                     CKGAYLSISHANHVRSTNELVLYNQLNGKYTHTNAFGTEVLVQLQQGETWGVNKTVKV
                     NVIKIEQNKGNMQIPAGYAVLSGHGTAATYLNSFSVGDEVAITQNVNINGVANPYSEV
                     VGGDHRTMMLRNGIPTTDQIWNELHPRTAFGYSQDKKTVIQCVVDGRGISVGVNTKEL
                     AEIMLSAGAYDAINLDGGGSSCLFVKNFGPMNKTSDGSERAVANGVFVVSTAPTDNTI
                     TEIRPYETTIRLPQYGVLRPKYLGYNQYGMLINKDVQGVVLNCSPELGYINGQGQFVA
                     SGGIDGVLNATYNGNITTTANIHIVQDAEIFMRLDSVLIDSRFEYPVEVQSTIGINTM
                     TVYPASLTWIVKDPSICSVTNGVLKGLKNGTTMVIGNLGSFSDTINVKVEIPESGRII
                     FDKFIPDRWKLEASSALNATLCTTNLPVGWDHGAAVNYTYVTTRAPYIKLLNKNLLYS
                     LPDTFKLRLNVGDIALTKVVFTLRANNSAQNVSKEFTSVQNNADTEISFPMSLFFNVA
                     DIAAFPVWFENVNFYLGVQTVNQAYTLAVKDIVLCYKGYTVSYTSPEKMDIFQVYPNP
                     LKGNMLNIKLIKPDEGAINVQMFDASGRLMMSQKLEADFNGNASLPVSGIPSGTYFLQ
                     LEQSGKSASVKLMIQK"
     gene            complement(8180..9679)
                     /locus_tag="PKH58_01780"
     CDS             complement(8180..9679)
                     /locus_tag="PKH58_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87786.1"
                     /translation="MKKKLLFSIAILVGAISSTSYGADVSKDPDAYANRSNYKLESKW
                     MYSAVLNNYNVAADKLGAAGAVRGMTAKNGKMYFCSRTSVNQILVVDGATGAKLDPIN
                     LESNVFTYLGRNKANTADSTWTAPLPNNDIQKDNAGNILVSNLITSNTGRFQVWKIDI
                     ATGGGTILIDTDIITHYPLSKTGTRFDAIGVWGDVNNNAVIMAANAEATVTEVYKWTI
                     TNGVVNQKPDLIELDNSGQYWFGAYTTATNTFAPLGNLGSAPRVLPVDDTYFYVDGNS
                     TYPTLCDKDGNVIDGFYKIFNPDGTTQDATAYGLLTDEITSPSNQWVMNQGHNGVKEF
                     QIGTDYFLIMAGSNTAAKPFSSFRLFKFKDASKLFKDMTLMWTFPQAGMGAVSNTVRT
                     GMPDVEVTGNSANIYLYTHENGYGAYTMTISTGINNINASNVDIILRGRQIVISEQVK
                     SAEIYSVVGQKIASYSNVSVMESPETKGIYIVNIIDKNGAKKTQKISVQ"
     gene            complement(9746..12493)
                     /locus_tag="PKH58_01785"
     CDS             complement(9746..12493)
                     /locus_tag="PKH58_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNX87787.1"
                     /translation="MKKILLLSIILSIAFSGFSQSPKREFRATWLTTVWRNDWPKSTV
                     ITSTGNSGEIQMQKNEMIGILDSLKRMNANSVCFQVRSRCDAMYKSSFEPWSSDLVAD
                     RGMDPGYDPLAFIVDEGHKRGIEVHAWINPYRFESVNNQWSGKPGDYRTTHPDWLLTY
                     SATNNSILNPGHPEVLLQIKKIVAEIVKNYDVDGIIFDDYFYVSGTTDAMDDAQFKLY
                     NPDKLSRGDWRRQNVNKMVRGVRDTIKSVKPYVKFGISPAGVAASSQVVANKYGVTPC
                     PGSDWQYNDIFSDPLAWLSQGTIDYISPQIYWTIGSANDYTKLCTWWSFVANKFGRHF
                     YSSHSLSAMTAGGASVPKMMKVAGQQTALSSMSGIERIATQWNSNISYAPTATNFYAS
                     EIGNQVNNNRQEDLNGAPGSIFFPTKPVQNIAGFINYIRKYQFVKPALTPAINWGQHP
                     EYSLVSGIDLSGNALTWNDAGPNLRYTVYAIPNEKVNDVGNFSVSNYLIGITYQSSFT
                     VPANISTTTNTFAVAILDRYGNEFPPLVMGKTPGTQIIPQLVYPADVTTVFPPFEFKW
                     NSSDLVDYFTLEVAEDANFNQIIYTRDLTANTFSSTNINYLKDNGKYYWRVKTRKANT
                     PDGVSEVRTFTLHKFAINLPTNGSTGVSLTPTITWDNVGPTASYILELSTSLQFTSIL
                     YSTTLATNTTTIPENVLNGNTTYYVRVKVTFGDLQTTTDVVSFTTLVLPVTVPEITKP
                     LNGETILTSSVEVCWKQQNSNGFRAELSESSAFPPRSTTVKTTNANTNCTVFDNLVFG
                     KMYYIRVFALTTTGMTASSTVISINLNTAQSTLSEELSDYYIRTRPDKVSELVIQSSD
                     NCNAQIVLYNITGMLLFDKKINLSFGKNVIEFNMNELGSGIYHLVIKTDKYKRTIKIK
                     R"
     gene            complement(12506..14161)
                     /locus_tag="PKH58_01790"
     CDS             complement(12506..14161)
                     /locus_tag="PKH58_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX87788.1"
                     /translation="MKILPYLLTLVTAFLVFSCIDNEPDIEELPNPNIAFKYSVIDPT
                     YQIDYYVGATIEFVSTSVAVGECTWDFGDGTIATGSTVTHKYKTAGTYTVKLTVANAG
                     YQTKPIFISDIKPIVSLNKTGLIEVISSSVGFNVELPNPEGLSEEYHWIFPDGTKDAA
                     GTIISDFNGKDPGNLTFSNIGSQTVTLQVKLGGRQLEDGVIKVPVAYNQAVPTLYYAV
                     KGGNIMALKLVSNAPAGMKINPFDMGTKSGQHPLNILFSDTSLYVIDCGRQFTYVDDQ
                     DGNLGDGRIFVMSKDGAKVETMLTNIGAAFNDPYYGYIDGGQLYFSDRNTGISTIGLK
                     ERNRTFSRADFPYFVQNATLGYYGQGLSYGAMNACFGKINNVWYWCKTYNGNGIFRFL
                     DTDILKTPITGNGVLPLSGIALESMTPKSLVWDAKNKVIYFTLYDTGYEGLYRCTLEQ
                     LNAIKARADLTPYRLKLANGKTITPITETGKGEGSAGEFITICQLALDENDGSVYFGL
                     RSGDAAVKSGLMRYNPTSGFIEYVIEGIDVYGVAVNKTKSKLF"
     gene            complement(14172..15020)
                     /locus_tag="PKH58_01795"
     CDS             complement(14172..15020)
                     /locus_tag="PKH58_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87789.1"
                     /translation="MKTRYNIIFFIAAIFLFVACNKHDMMNDVVIVGQMAPQVYWEPA
                     SSTVKAGEDIPFTVQYYTTSKENIERLEIWYNILEDESKSVSSPWVTTFTYSVISNKS
                     SEKRVSQKIATYDHNPNYWNDSLRAYTFKSTFPTSNTLASVTWSKPATFDNDKMVKYF
                     GNAFMTQFKDSLFKLMKAKDFQKMYLGLNLLDNFKMYIDSVKNDNTGSWDYVFPKDAQ
                     GNRPVPKTLVDIYATIPFSDIIFNKTTNVYDVDYSRSYRINANMKAYDKQGVAGLGIV
                     KEISLN"
     gene            complement(15042..16808)
                     /locus_tag="PKH58_01800"
     CDS             complement(15042..16808)
                     /locus_tag="PKH58_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX87790.1"
                     /translation="MKAYKILFALFISSVFLFNSCQDYFDINTSKDTPSEVSPEQLLP
                     VLVFYSSQICYDHAEYGTYLSQALTTGGRSQTSSYAYKAGWELLSMNRHPQWRRHFYD
                     IGVNADALIKGAEKIDSRNYILIARTIRLESTLFTTDAFGDIPLSQVYQSNSPSYDTQ
                     ESVYEWMNKEADELISLYENPEWINNKNNRSISGLQDRLFGGDLGKWRAYAKALKARL
                     LLRMIPNWKNTLENCQKIIDAADAALNDPSYADALYHYDGGSTEKNCPWGPAQPKLNL
                     GWAQARENLLTDAIPSKFFAYALLSVYSNPRQTIGFALDPRAEQLMTPRLDDKNLNAI
                     RYIENNIGMPTTMKITFYPDLYALQGGNPYTKNNGYVMLMSREELLFIKAEAQYWSGD
                     KTGAYNTTVDAVKSNMRRVGVKVDDPNMKPFIDRFFLVRLPGAATFTIADLMQQKYVA
                     MYLQPEQWTDMRRYNYSSKNNGIAYDGVFVYHVTRIHDGTRNVNPTNFTQEFSLTRPF
                     NLYSAYWIDKPDNFGVNAKFSPNAWITRLNYDPETEDKYNRKELERLGAYKNPDWLRK
                     RMIWAYKNNSYVTSADPDIEWK"
     gene            complement(16820..19942)
                     /locus_tag="PKH58_01805"
     CDS             complement(16820..19942)
                     /locus_tag="PKH58_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX87791.1"
                     /translation="MKKFYILALLSLLFFSPVLAQNIITGVVTSSEDQMPVIGVSVAV
                     KGTTRGALTNIDGRFSISASKNETLVFSYVGMEKQELKLGDQTVVNIVLKPVAEFLEE
                     VVVTAMGVKAEKKKLNFAVQTVNSDALTEGRQSNFVNGLQGKVSGINVTNSGGSPNSG
                     SLLIIRGISSINPSQSNAPLYVLDGVAISGGVSDINPNDIENITVLKGAAASALYGQS
                     GANGAVLITTKQGVVGKTTTNVNASIQTNQPIRTPAKQSIYGPGTLGFYKPEASGGYG
                     GWGPLLGSDEPRYDNIKNFFKDGFYQKYDINVSGGSEKVKAYASAFYSKDEGIVVNDY
                     LNKVGILAKGSYDISKKIVFSAMANIVNDVYRGAGGISSAYSWPINDDITNYMENGKV
                     RYRYISEKLKSGSPISPLFSRYMDYGKNDNLRAIMQGAVNYKAFKNFEAIGRVNFETN
                     SYLYDGYAVPRFDDSVILPDAPKADQYGDNTDAYAQALANYNSNYYKSPYFNQADYAN
                     VDKNLLGNYSHSNSRSQYLTLMGILTYKINLPSELSMDLLLGGELKTRDGISSSINGR
                     DFIVPGIYSLENVKEIQGVNDIGVSHTQRRNGGIFDQITLDYKGMASISATSRWDWSS
                     TLSQEFSPYFYPSITGGVLFSELFKLSNKWFNYGKVRGNWARVGKDTPAPFLFDRKYT
                     QYPTLPDGGYGVDPSKSVASLLRPEMSDSWEIGLETRLFNNKTHFDFAYYSTYVDNQI
                     VTVRVSPASGYILQTRNEGTVENHGVEFLWEQDILTNRDFTWKSSMTFGFNRGKVVKL
                     PDDIVEIQGTQYTDVFPTAYLNGSTTAISGKDYLRTEDGEVIVNTDGYPKINPTKSVL
                     IGNREPDFLMGLNNSFYFKGINVAFLIDGRKGGDIYNATSRGMWSDGQHKDLEFYRGR
                     QVVWDGVVQQPDGTYTKNTTPIVLDSKTITEYYAGVSSNFIEDGSYIRLNYLTIGYNF
                     SSLIKNKTQFTSLKCSLTGSNLFLLTKYTGSDPQINANTGSGGTGGMGIDYYPVPPTR
                     GIHLTVSATF"
     gene            20353..20838
                     /locus_tag="PKH58_01810"
     CDS             20353..20838
                     /locus_tag="PKH58_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX87792.1"
                     /translation="MKKDIFLHSLFAGLILLSSINSYGQSSLCIFKNDDSQINSWLIS
                     QIKITFSENNLLVYNGNSTTNIPLNNIRKITFSSTNTNVSTENFSNSLTLYPNPVSNI
                     LHLKNLNSENLTVRIFSIDGKAVLTRTLSTYHTDIDVTILPKGLYILKANDQTVRFTK
                     K"
     gene            20835..23717
                     /locus_tag="PKH58_01815"
     CDS             20835..23717
                     /locus_tag="PKH58_01815"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNX87793.1"
                     /translation="MKKTILSILLIAGVISLFASEYIFIFKNDHSVTSIAISAIDSIK
                     FSDDQTQLNIHKSDQSLIHYSVSELDSITFGSIYTPDVSITYNNSVVTISNPYQYKGI
                     NITTSGADVIVNSTLAGQEINYLISGATSDGSIKIYSNNKYTLTLNGATITNNDGPAI
                     NLQSTKKCTLTLASGTTNTLADGSSYLSSTEDQKASLFSEGQIIFKGTGTLNVSSKSK
                     HAICCDDYIEIEEGNINVTAASKDGIHAKGHFNLEGGAVTVTATGDAVECEAGSIKVS
                     GGSISSTISSADTKGLKSDSTLLVSGGTINFTVSGNQAKGLKAGQNMTLSGGNINITT
                     SGAAVLVASGSGFDPSYCTAIKCDSSILISGATINITTSGVGNKAISTDKNLDITSGT
                     VKIVGTGAGATYTNSLGVIDAYGSSGIDADGNVSILGGNISVSESGSAAKGISTDGNL
                     NIGNASNSPIIYVKNIGTKLLISGTANYATAVYSEPKNIKSDGIMTIANGTIGLNATQ
                     PGANTIDCDSLLNVTGGVINDTISGNQSKGLKSSKAMTLSGGTINVKASGAVVLEYTT
                     STTLLAPSYCSAIKSDDDVLISGANITINHSGAGGKGISADKNIEMTSGVVNITTTGG
                     GAKYLYATSTYDSYSAAAITADANISILNGNLTTSSSGTGGKGLKANGTLTIGSSTQS
                     PTVNLNTTGTTFTVSGSDLCHPKTIVTDGAITIANGTNTISSTDDAIHSETSITISGG
                     NNTITKATEGIESKYIYITGGENNITASNDGLNGTMGTVSGGTESNDNSLISISGGTT
                     YVTGADAIDANGNFLMTGGIVFSNGPASGAEEFIDANGTAKINGGVFVGCSSAGMLKT
                     STFSSSTQPFLYMTTTLNSSTMYTVTTGGNWVASFKPKNGGGVCLVSAPQMTKGGAYV
                     VYTGGTYTGGTSINNFYMNGTFTNTGALIKKSATLSTTATLNQISF"
     gene            23923..24684
                     /locus_tag="PKH58_01820"
     CDS             23923..24684
                     /locus_tag="PKH58_01820"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNX87794.1"
                     /translation="MEKFLEILNELDPISLAEMDSVKLLNRMDVKYIFHKSEFADILT
                     VANEFYRTLTINERRFENYDSLYFDTEDFKLYNAHHNGRSNRFKLRMRTYIYSDLHFF
                     EIKFKSNQNRTIKKRVQLPKKTNEITGNALELLQSKTSMPVDDLKPALRIKCKRITLV
                     DKSLSERVTLDFDMKYNLGNDQWHEWPELVIAEVKQEKSGHSPFIKFMYTKHIRQISL
                     SKYCFGIANYINTVRKNNFKTRLHYVDKICKNHAI"
     gene            24650..25429
                     /locus_tag="PKH58_01825"
     CDS             24650..25429
                     /locus_tag="PKH58_01825"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNX87795.1"
                     /translation="MWTKSVKIMPFKTLSIILFMLLLMPSGTRSQPQISAAAAEAEQT
                     ASVQSNDESKDVVDMFTIDTKTEKKTKDKIQLNFNFFFSLLIDLAFLALIISLIYYPN
                     YKRLDTVFTFVLFNVSIFLLTFLLNHIKISMGAAFGLFAVFSMLRYRTAGISVKDMTY
                     LFIFITMGLLSGIQLELYELVIIFSVLFVVLIVLDTRILLKRESTKIIYFEDVELLHT
                     DRHPELIEVLKQRTGLNIHRITVEEISYLRDFGMITIYYYE"
     gene            25429..26136
                     /locus_tag="PKH58_01830"
     CDS             25429..26136
                     /locus_tag="PKH58_01830"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNX87796.1"
                     /translation="MTTKKNFLIFGLLIAMSGVLNAQNQYGTWTSAGLEKKAGNWNFG
                     AETELRTIHYVRLINRWSLSMNAEYKIAKPLKVGIGYEFMNVLDEKYLNYQFRNRFNA
                     DISGKQKLGNFAFSLRERIQLTKKNDSKRIDEDGYNDTYKIDPAWVWRNKLNADYNIP
                     KCRFTPGISVETYYSLNDPDGNKLDKLRYILYTDYKINKYNKIKIFGVLNSEKGTDEA
                     DYSGKYNLGLSFSHEIK"
     gene            26187..27686
                     /locus_tag="PKH58_01835"
     CDS             26187..27686
                     /locus_tag="PKH58_01835"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNX87797.1"
                     /translation="MKLKSIYFLFAFSALILSACDPENVLKPAEETIKPLYEWDETKI
                     QTITFQNSAVSTTSSKVVITGATATITDGGYYTVSGNSSNGRLIVDAKDEIVKIRLAG
                     LTLSNDTTSPFFIKDAEKTIVFLKSGTTNTFSDGASYTHTGDPNATIFSKDYIGFTGD
                     GNLVVNANFNDAISTDDMLYIDNGNFTVRSLDDGIRGKDSVVVNNGNINIICSTGHAL
                     KSDNELKADKGYLNIKGGTLNLSSIDGDGINTTKRLIIEGGTITAQSTNSQALRSDSV
                     VLISGGITTVNASKKGIESPYITISGGTTTINSNKAGVNATYGLGGSQIDGSILNISN
                     GILYVNSVSGTGLDSSGDIKMSGGTVVVQGPSDLSKEGIKYNGIFTITSGFLIASGPQ
                     STISVKAPSASSVQNTVRITSTTIGTNLIAIQDASGNTIAAFKPTRSAYGLLLSSPEL
                     KTGTNYSIFKGGTYAGGTTVNGFSQNGTYSGGTLLKTFTVNDKITNVSF"
     gene            27917..28942
                     /locus_tag="PKH58_01840"
     CDS             27917..28942
                     /locus_tag="PKH58_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87798.1"
                     /translation="MKKELSFAFFAAAIFLSLSLLSGCTTYNYGTTNEQTYYENPQWA
                     PDYYTGTRYYYFPDIECYYDLATRDFIFLNRGQWYFTPRLTDFYSDFDLFNSYIVIVN
                     VNVYQPWMHHQYYVSHYPRYYYRDYYDYSNIPYVRGFNENVKSAVYWPEQQRYRAREW
                     NDNNLRNNRRFKYSDADRQMQVNTTNRINNQRSEDVNSSRNTGTGGRRSNEKYTQPRN
                     TNTQTTQSTNTTTRPANTTNTTSTTNRNTNTERTTNQAVDTNSRTTTTTRSTASDSNT
                     PVRQSGTVTRSSERTANTNYYGKPIGQPVKVERRMRSTTTESKAEEKPDTTTLTNTTT
                     RTRSSRY"
     gene            29143..31341
                     /gene="pnp"
                     /locus_tag="PKH58_01845"
     CDS             29143..31341
                     /gene="pnp"
                     /locus_tag="PKH58_01845"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNX87799.1"
                     /translation="MNVVTKTITLGDGREITIETGKLAKQADGSVVVRMGNTMLLATV
                     CAAKDAVPGTDFMPLSVEYKEKYGAAGRFPGGFTRREGRAGDYEILVSRLVDRALRPL
                     FPDDYHAEVFVNVMLISADGVDMPDALAGLAASAALAVSDVPFHGPISEVRIGRINGE
                     YIVNPTFEQLENADIDIIVAATLDNIMMVEGEMSEVSEDDLLNAMKVAHEAIKVQCQA
                     QIELMEAVGKIEKRTYCHEENDDDLKKDIWEKTYNKAFAVATSCNTDKHSRSENFEAI
                     VAEYIAGIDPEVAEAKKELIGRYYHAVEKEAMRRSILDEGKRLDGRKTTEIRPIWSEV
                     DYLPGAHGSAVFTRGETQSLTTVTLGTKMDEKLIDEVLQQGKDRFLLHYNFPPFSTGE
                     ARPSRGVGRREVGHGNLACRALKSMIPDGYPYVIRIVSDILESNGSSSMATVCAGTLA
                     LMDAGVQMKKPVSGIAMGLISENKGQNYAILSDILGDEDHLGDMDFKVTGTRDGITAT
                     QMDIKVDGLSFEILENALNQAKAGRMHILDKIQETLTEPRPDFKPNAPRIVVMMIPKE
                     LIGAVIGPGGKIIQNMQAETGAVISIEEIEDQGRIEIAATNKASIDAAVAKIKGIVAI
                     PEVGETYPSKVKSIMPYGAFVEFMPGKEGLLHISELDWKRIETMDEAGLKEGDMIDVK
                     LLDIDQKTGKFKLSRKALLPRPPREDKPREERSHDEKPREHRGPRPERRD"
     gene            complement(31431..32524)
                     /locus_tag="PKH58_01850"
                     /pseudo
     CDS             complement(31431..32524)
                     /locus_tag="PKH58_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644494.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
     gene            complement(32677..33534)
                     /locus_tag="PKH58_01855"
     CDS             complement(32677..33534)
                     /locus_tag="PKH58_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX87800.1"
                     /translation="MANNLLKGKRGIVFGALNDMSIAWKVAERAVEEGAIITLSNTPL
                     AVRMGTTNELAEKLNAKLIPADATSVTDLEEVFKQSMEALGGKIDFVLHSIGMSPNVR
                     KKRTYDDLDYDMLSSTLDISAISFHKMLQVAKKMDAINEYGSVVALTYMAAQRTVFGY
                     NDMADAKSMLESIARSFGYIYGREHNVRVNTISQSPTVTTAGSGVKGMDGLLDFSERM
                     SPLGNATAADCADYCMVMFSDLTRKVTMQNLYHDGGFSSMAMSYRAMEQYNKSFDEFK
                     DENGKIIYG"
     gene            complement(33817..35976)
                     /locus_tag="PKH58_01860"
     CDS             complement(33817..35976)
                     /locus_tag="PKH58_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNX87801.1"
                     /translation="MKVYQTNEIKNISLLGSSGAGKTTLVEAMLYESGVIKRRGSINS
                     QNTVSDYFPVEKEYGYSVFSSVISYEWKNKIMNFIDCPGSDDFAGAVISALNMTDMAL
                     MLIDAVNGVEVGTINQFRYTESLHKPLVFLINQFDHEKADYDNTIANLKETYGSKVVP
                     IQYTIGSGQGFEAIVDVLKQKMYKWKPGATAPDVMDIPASEKDKAEELYKALLEAAAE
                     NDENLMEKYFEQGSLTEEEMREGIMKGMIDRSIFPVFCASAEKNMAVHRIMNFLSMVA
                     PSPDQMPSPVTTAGVEVKPDAKGPTSLFFFKTTVEPHIGEVSYFKVMSGKVKAGDDLV
                     NISRSSKERITQLYSVSGQIRNVVDEMVAGNIGATVKLKDTRTGNTLNEKGCDYDYKA
                     IKYPDPKYRRAIRAVSESDEEKLNEILTRMHEEDPTWIIEQSKELKQTILSGQGEFHL
                     RTLKWRVENNDKIQIEYKEPKIPYRETITKVARADYRHKKQSGGAGQFGEVHLIVEPY
                     YEGMPAPDMFRFNGQEYKISVRDTQTVDLEWGGKLVVINSIVGGAIDARFIPAIMKGL
                     MDRIEQGPLTGSYARDVRVIVYDGKMHPVDSNEISFRLAGRNAFAQAFREAGPKLLEP
                     IYDVEVFAPSDRLGDVMSDLQGRRAIIMGMHSEKGFEKLSAKVPLKELSSYSTTLSSL
                     TGGRASFTMKFASYELVPMEIQDKLLKEYEATKKEEE"
     gene            complement(36444..>37762)
                     /locus_tag="PKH58_01865"
     CDS             complement(36444..>37762)
                     /locus_tag="PKH58_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNX87802.1"
                     /translation="DGTSPYSEYYLADPYLQGIKSGKYSEKELNQKVTRLLRLIFRTT
                     MAPNRPFGKFVSPEHSEAARKIAEQGIVLLKNDKQTLPIPVGKYKRIAVIGDNAVRSL
                     VIGGGSSSLKVAYEVSPLEGLIAKYGAEAIAFSRGYSPDLDYRKPESKIIADSLLASA
                     VETARNADAVIFVGGLNKNWFQDSEGDDRQSMALPYEQDKVINTLMTVNKNLTVILCS
                     GNAVEMPWLPQVPALIQAWYLGSEAGNAIANIVSGEVNPSGKLPFSFPKKLEDNAAYS
                     YGALSYPGKDNKQEYMEDILVGYRWFDTKKITPQFPFGYGLSYTTFSYGKPSTDKTEY
                     QANDIIKLTFQLSNTGKTAGAEIVQVYASQPKASVLRPVKELKAFKKVQLNAGETTTV
                     ELKIKTQELAYYNNKTQSWVVEPGEFILQTAASSANIKGKVVVKIK"
BASE COUNT        11356 a         7845 c         6961 g        11600 t
ORIGIN      
        1 gcaaaggaac ttcaacatat ttccagaaac cgtaatggat gctgtccgcg tggataaacc
       61 gcatatcgtt gccggaggta aacatcaggt aaagctcatt tcccgacaaa tcgccataga
      121 ggtgcagccc catcaccgag tctcggttca catttaatgt cggtgtattt aatttataaa
      181 ccacttcacc tcctgtttta tcggaaaaag tatatttcag gttataactg tatgtatcga
      241 acaagcgggt ggacgacgaa ctttgtacgg atgccgaagt tacgttcctg ccttgtgtct
      301 cgtccacgct gattttaccc gcggtttcga aattttcagc taccattctg tcggttactc
      361 gttcgtccga tgctgtgaac ggaattttca gcgtatcggt caggtacact ccatcgatgt
      421 ccgtaatgcc cttagccagc ttaaccacat actcctgtcc cggtgttaag tcctgcccga
      481 cgatgaatga agttgatcca taaggagtct gtacggtatt gtgacgcaaa gaacgaagat
      541 ttttagtaat tttcttcccg ctcttatcat acacctgtac accatctatc aattcggttg
      601 tttgcagttt tttatcaaat acaaacgtaa ataccggcca cttgtaatca acatgcgttt
      661 cgtccggcat cggatatgat gccaacagtt tcagttcatt acggcttctt gttctgaact
      721 ggaaaacaaa atcattttcc atatacaggt tatcgggatg tctgactgtc tttgccagtc
      781 gcacagtata tattgtcgat ttatccagtg gcacacgggg gatgaattcc atcaggtaat
      841 tcgcgtcacg gaaaacgata tctccatcga tatgcggagt aatgctgaat gcttcgatag
      901 ccgatgtttt atcaacatcc cagttgaaac gcatccagat agtcgaacca cttgaaacac
      961 tatcagtaag tgtcacattc ggcgaatact caaccacttg cggaggtgta ttcctgattt
     1021 tattcatccg gacagaggca taactcgttt tatttgcttc caccgttatc tgcgtggtgt
     1081 cgggatgata agccggatgt cgtgttataa ggctgtatgt tccggcggga agattttcaa
     1141 aagagaaaac cccgttgttc aaattatcgg ttgtgcaaac cgaatcgccc ggaataatag
     1201 taacggttgc gccatttacc ggcaggtaaa gatcttttga atttgctaat ttgtaatagg
     1261 aagcctcatt taacaaatac ttgtctctga ccgaacctgc aatattaccg gtacttaatg
     1321 cagccgtctt aaagtatgtg gcaaatgatt tcagaaaatg ccatgcctca agtcgcttat
     1381 agtccatatt catcagacgg taggtttccg gaatataatc atgcatagat ccttcggaaa
     1441 tcacgcccgg gactgtaagc cccctcaaga ccccataccc atcggaccag ttatccatgg
     1501 caacacgtcc gaaagttttg tcgccccgaa cagaatatga ggagctccag gcggtaatct
     1561 gatttgaata gaggttctta gcaatctctg tgcttatcgc cttgctgagt tcagaataag
     1621 gtgtgggcgt tggatacacg tgcgtatcgc ctgcatcgac accggaatac agcatcagta
     1681 catgatttgc cgaaccggct ccggcattgc tatgaatgga aagcataaaa tcggcattat
     1741 actcgtttgc catctgcaca atagctgaca gaggcaaatc gtcggatgta gtattcttcg
     1801 ttctagacat catagcatca gcgttgtggg tcagcaacat actacgtagt gtaaatcctt
     1861 tatccagatt tgatttcgat tcccagaaac cattttgatc gccggaagta aatggtgcaa
     1921 tcactacatt acggtcgtcc gaatcgtaac cgccatgacc gggattgata aatatccgta
     1981 cgcctgacaa atcctgaccc tgagcagggt taataaacag cagtaatccc gctaagatta
     2041 ttataaattt cgatgctttc attatttcac aggatttaca gttagaataa aaatatctcc
     2101 ttcgtaggta ttatatgcaa tctttcccga caccgcggat gctgagggat acatggccat
     2161 ttcattatca tcggtcaggt attgtttcgt ttttccatcg gcagctacca ttagtatcct
     2221 ggatgaagta tatacatact cgttgtttct ggcgtccata cctacaactt ggttgtcatt
     2281 ataccagcgc ggtgcctgaa ggtttcccag ataggatatc ggattcccgt tgaggtcggc
     2341 aatgtatgta cccttgcctg tcgcataaaa aagcattctc tttttatccg gcgaaagaga
     2401 tgcccatata tacccgctca cggctgtttc gtcgatgggt ttcagttctt tctcaatgcc
     2461 gttgacgctg actattacag attgcgcgtt gctatataca cgtatgtctg tttctgttct
     2521 cgctgctgtt ctcgctgcag gcggcaaagt tgatttcaga ttacgggtcg attgaataag
     2581 cacacttcgt tgtttattta ccggattgta ccgttgtacc gactgatatt tccgcctatc
     2641 cacaaagcgt gtttcacgat gcaggatatt tcctgttttg ctttcaaaca acggttcgaa
     2701 acctgcgcca tcagcatcgg atatcggggt tagtttatta tttgtcagat catacaattt
     2761 cagtcctttg taatttacat ctgtgtataa aagtttatcc cccttttcat tcagcacggg
     2821 ataaaacacg gatttctcaa tcccgacttt ttcctttttg gttattttta ctaattgtcc
     2881 ggacatattt gaatacgcca tcagacaaac gaataataac attactgttt gtttgttttt
     2941 catgtttatt taagtaaaat ttttacagat tcggtttctt gcttacggat gacattcacc
     3001 aagtacattc ctttattcag tccgtccaga ggtagtttta cctcaccgga ttgcatattg
     3061 gtcagcttct ttgatcttaa aatctgccca ttctgcccga caacatctat tcttacatct
     3121 tcaggtctat ccagcttcag ataaatgtag gaaattccat cttttgccgg attcggatac
     3181 accttaaaca tattcatttt tacgggtgca gtatatgata ttacaaaatc cttaaatgac
     3241 aatgaaactt ctttcactgc cagagaatag gatttctgcg cagtcatacc ggcggcgtca
     3301 atataaaaat tcacattatc aaatttgaca ggatatatcg cccggttgtt tacatccatt
     3361 aactgatcca ccggtatatt cagtgaaatg tctttattct gttcaagcgc agtgaaatca
     3421 cgtgacacaa gtttaatatc gttatttgcc tgtaacgata ctattgcctt tgtcagcgca
     3481 atatcgccaa tattaataac cagttttacg gtatcgggta aaccataaag tgtactttta
     3541 ttagtaagtt tgatagacgg agctctcccg gctgcatata caaaattaac taccgaaccg
     3601 gttttccaac cctgtggaag actttctgta ttccattgag cattcaggaa ggtcgaagct
     3661 gtcatagccc agtcagcggt atgaatgctg tcggctatca tggatggtgc agccggtatc
     3721 tcaactctga caagcagtgt atccttaaac tcacccaggc ttccgtaaac aaaggttttt
     3781 ccatttttca atccctttaa tatgccatta cgaacctcac atatcgcggg atctttcact
     3841 tcccagctca ggaactccgg tgaaactgac atcaaagtac ctgttgaatt actaataacc
     3901 tctatcggat aatcatttct tgtatccatc aaaatcgaat cgcatttcaa actgacctgg
     3961 ccactgacta cttctatcgg tttggtagct gtgacaccat taaatgatgc tatcagacta
     4021 cctgtcccgg gattacccga agcaactaaa gtcttgtttt gagaaattgt tccaatttcc
     4081 ggggagcagg acaaagtaaa tcccgtcaca tttttattga caagtacgcc aaaacggtta
     4141 tagcctaaaa ctacaggtgt aaatgacgaa tatgaaggta tccggatttg atgattcgcg
     4201 aactcaatcc tggttatcac attatcttcc tgagcattat gaaacaagaa ccacccattt
     4261 gcaaccggac gttcttttcc atccgaagga ttgttaacta tctgtccatc gagcatcatt
     4321 tgtgcagaac ctcccccatc catcgatgtc gcatgctgtg ccccagaggc tttcagaatg
     4381 ccacacatcg tttccgtact ggctcctgct gattttccac ctttgtcaat aacgataaga
     4441 tagagagttt tcccgtccat tgaacttccg attccagttc gggggtaaag ctgcgtatta
     4501 taagcttcgt tatagtttct gttagtaagt gttccatctt tcattaccag ggcattgcct
     4561 gttaccatct gagcaactga cggaaattga ttatcggtca tggtctttac cgccatattc
     4621 acaaacagct tatcgcctgc cgacaggtta ttcaggaaag ttcttgcagt tccgttgcct
     4681 gacaaaacgg tttcaccttc catcagacta ttgcctcctt tgtttgaaac aatacgggtt
     4741 accacacatt caacatcact gtttacttcc catttcttcc ctgtcaccgg tttaatgaag
     4801 acttctattc cgtcatcgtc ggtttgtgtt acctttgacc ctaaaaagtt gttgaaaagt
     4861 accaactcat tgatatttct taacctgtta atctgcgaaa caggatactc acctttaccc
     4921 ggaattttta cagttccgac aaaactaata tcgtctatcc atgctttctt atcagaatcg
     4981 atcatagcaa aaccgatact accatgtcca cgattccagt tattggggtc agttatcatt
     5041 tcaccgttaa aggcacttcc actgtgtgga actcccagaa gtgcatccgg ttgtccctga
     5101 cccgagacta tccagaagtt tccgtttaca cccgcaattg tgcgatgctg ttccctgtca
     5161 agccggctat aagcagatga catagcttca gtctttccta cctgattttg tgcctgaaaa
     5221 gtttcgacct gattatacgg atttgttaag tcaacaacca gcatatagcc ggaaagcgga
     5281 taatccggta atacgaattt tgtgtattga attcccggac caacagattt atgttcaagc
     5341 gtgtcaatat tcttcgaaac tcctaaaatt tcaatttttc cggcactcaa attaatgcta
     5401 ttaatcagga aaactgaaac taacaatatg atatgtaatc ggaggctttt cattttctat
     5461 ttttgtatca ttaatttcac ggatgccgat tttccacttt gttccagttg aaggaagtaa
     5521 gttcctgaag gaatgccgga cacagggagt gaggcatttc cattaaaatc tgcttctaat
     5581 ttttgtgaca tcatcaagcg accggaggca tcgaacattt gaacattaat ggcaccttcg
     5641 tctggtttta ttaatttgat attaagcata tttcctttta gcggatttgg ataaacctga
     5701 aatatatcca ttttttcagg cgaggtgtac gataccgtat atcctttata acacaaaacg
     5761 atatctttta cagctaaagt atatgcctga tttactgtct gaactccaag ataaaagttt
     5821 acattttcaa accataccgg gaatgcagca atatctgcca cattgaaaaa caaactcatt
     5881 gggaacgaga tttccgtatc ggcattattt tgaacagaag tgaactcttt tgaaacattt
     5941 tgtgctgaat tatttgcgcg taatgtgaat actactttag ttaaagcaat atcaccaaca
     6001 tttaatctta atttaaacgt atccggcaaa ctgtaaagca gattcttatt aaggagtttt
     6061 atgtagggag cacgtgttgt cacgtaagtg tagttaacag ctgctccgtg atcccaaccg
     6121 acaggtagat tagtagtaca tagagtagca tttaatgctg atgaagcttc caatttccag
     6181 cgatcaggaa tgaatttatc gaaaataatt cgtccggatt cgggtatttc tacttttaca
     6241 tttattgtgt cgctaaatga gcctaaattt ccaatcacca tcgttgtccc gtttttcagc
     6301 cctttcaaaa cgccattcgt tactgaacaa attgaaggat ctttgactat ccatgttagc
     6361 gaagccgggt aaaccgtcat agtatttatt ccaatcgtac tttgaacttc aacaggatat
     6421 tcaaaccggc tatctatcag aaccgaatct aaacgcataa agatttcagc atcctgaact
     6481 atgtgaatgt tggctgtagt cgttatattt ccattgtatg tggcatttaa tactccatct
     6541 atacctcccg atgctacaaa ctgtccctgt ccgtttatat atcctaactc aggcgaacaa
     6601 tttaatacca ctccctgaac atctttatta atcagcattc catattgatt gtatcctaaa
     6661 tattttggac gtaaaacacc atattgtggc aagcgaatag tggtttcgta tggacgtatt
     6721 tcagtaattg tattgtcagt gggagcagtt gatacaacaa atactccatt cgcgactgcc
     6781 cgttcggagc catcactcgt tttattcata ggtccgaaat ttttcacaaa taagcagctt
     6841 gaaccgcctc cgtctaaatt aattgcatca tatgcacctg ctgacagcat aatttccgct
     6901 aattctttcg tgtttacacc aactgaaatg cctcttccat cgacaacaca ttgaataacc
     6961 gtttttttat cttgtgaata tccgaaagca gttctgggat gtaattcatt ccatatttga
     7021 tctgtagttg gtattccgtt gcgaagcatc atcgtacgat gatcgcctcc aacaacttca
     7081 gaataaggat ttgccactcc attaatattt acgttttgag taatagccac ttcatctccg
     7141 actgaaaatg aattcaaata ggtggcggca gttccatgtc ccgaaagtac agcatatcct
     7201 gcggggatct gcatgtttcc tttattttgt tctatcttta tgacatttac cttaaccgtt
     7261 ttattcacac cccaggtttc tccttgctgc agttgtacca acacttcagt tccgaaagca
     7321 ttggtatgtg tatatttacc atttagctga ttatacagaa caagttcatt tgtagatctt
     7381 acgtggtttg catgacttat agagagatat gctcctttgc aagttactct tccggttaaa
     7441 gtgagtgtgc cgatataagg aactttatta ctgtcgaaag acatcacagg tgtactgata
     7501 ggtcctgtac caacttcccc ttcaaacata cttgcacctg taggcatacc attataagtt
     7561 gatccggtat catagaagtc accattcgtt ccggcaaaat aaacagctcc ttcttttgac
     7621 tttctggtag ctgtagttgt gggtctttca gtcccgtaaa tggaatctct gcctaaagct
     7681 gtacgataag taatataggg attattgagg tcaactttca ggaaaaatat gtttaatctc
     7741 ttgtaaccac gaagttcaac cgctgtataa taacttccgg gtccaacttt aaagtttgca
     7801 acagtatcaa tggtgtatga ttttccatct atgacgatat tgccggcaaa gacaccggat
     7861 aaaatgaaca gaaatagaaa tgacgtgtat aactttctca tagataaact agattttttg
     7921 agtgatagaa ctcttaatga attaattgat attatttttt ggtgtaaatc aaccccgaag
     7981 ctcgtcgccg gtgtttctat taatcatatg aggaaacaga ctgacgatta tgtttccttt
     8041 tttgattaag ggggatctcc aaaaggggaa aaaatgaaat gaaaggaata ttcagatggt
     8101 atcttaatac catcgttcat taatgaaaat atagcataga gacggcagat agccttctct
     8161 atgctattgg aagttttagt tactgaacag aaattttctg tgttttttta gcaccattct
     8221 tatcgatgat gtttacaatg taaattcctt ttgtttcagg ggattccatt actgaaacat
     8281 tcgaatagga agctattttt tgtcctacaa cagaataaat ttctgctgat ttaacttgtt
     8341 ctgaaataac aatttgtcgg cctctcaata taatatccac atttgaagca ttgatattgt
     8401 ttattccggt agaaatagtc attgtataag ctccataacc attttcatgt gtgtatagat
     8461 aaatatttgc agaattacca gtcacttcta catcgggcat tccggtacgt actgtatttg
     8521 acacagcacc catacctgcc tgcgggaatg tccacatcag agtcatatct ttgaaaagtt
     8581 tagacgcatc tttgaattta aataatcgga atgaagagaa tggtttagct gcggtattag
     8641 aaccagccat tattaagaaa taatctgtac caatctgaaa ttcttttact ccgttatgac
     8701 cttgattcat tacccattga ttggatgggg atgtaatttc gtcggtaagt aatccgtaag
     8761 ctgttgcatc ctgagttgtt ccatccgggt taaatatttt ataaaatccg tcaataacat
     8821 ttccatcctt atcacacaat gttgggtaag tgctgtttcc atctacatag aaatatgtat
     8881 catccacggg aagtacgcgt ggagctgaac caagattacc taatggtgca aaagtgttag
     8941 ttgcggtagt atatgcacca aaccagtatt gaccgctgtt atccagttca atcaggtcgg
     9001 gtttttgatt tacaaccccg tttgtgattg tccatttgta cacttcagtt acagtagctt
     9061 ctgcgtttgc agccataata actgcattat tgtttacatc tccccaaact ccgatagcat
     9121 caaagcgtgt tccggtttta gacaatgggt agtgtgtaat aatatctgta tcaataagaa
     9181 tagtaccgcc accggttgct atatcaattt tccaaacctg aaaacgtcct gtattggaag
     9241 ttatcaaatt tgaaaccaaa atattaccgg cattgtcttt ctgaatatca ttgttgggca
     9301 gaggtgctgt ccacgtacta tcggcagtat tggctttatt tcttcctaag tatgtaaaga
     9361 cattactttc aagatttata ggatctaact ttgctccggt agctccatca acgactaaaa
     9421 tctgattgac agaggtacga ctgcaaaaat acattttccc gtttttggca gtcataccgc
     9481 gaacagctcc ggctgctccc agtttatcgg cagcaacatt gtagttgttc aaaacggcag
     9541 aatacatcca cttgctttcc agcttgtagt tagaacgatt tgcatatgcg tccggatctt
     9601 tactaacatc ggctccataa cttgttgaag aaattgcacc caccaggatt gcaattgaaa
     9661 agagtaattt ttttttcatg attgtaataa tttaaatgaa taatatttat ttatattggg
     9721 aaaatctcta aatatcaaag aatgcttatc gttttatttt aattgttctt ttgtatttat
     9781 ccgtttttat gactaaatga taaattccac ttcctaattc attcatatta aactctataa
     9841 cattttttcc aaaagacaag ttaatctttt tatcgaataa taacattcct gttatgttat
     9901 aaagtacaat ttgcgcatta caattatcgg atgattggat tacaagttcg ctcaccttgt
     9961 ccggtcttgt ccttatgtag taatcagaca gttcttcact aagagttgat tgtgcagtat
    10021 taagatttat ggatattaca gtcgatgaag cagtcatgcc tgtagttgta agagcaaaca
    10081 cacgaatgta atacatttta ccgaaaacca aattatcaaa aacagtacaa ttagtgtttg
    10141 cattagtcgt ttttaccgtt gtactgcgtg gtggaaaagc acttgattct gaaagctcag
    10201 ctctgaatcc gttcgaattt tgttgtttcc aacatacctc cacactgctg gttaaaatag
    10261 tctcaccatt tagtggtttt gttatttcag gtacagttac cggaagtacc agggtggtaa
    10321 aacttaccac atcggtagta gtctgtaaat ctccaaaagt aaccttaact cttacataat
    10381 aagttgtatt cccattcaat acattttctg gtattgtagt ggtatttgtt gctaaagttg
    10441 tcgaatataa aatactcgta aactgaagtg atgtggataa ttcaagtatg taacttgctg
    10501 ttggacccac attatcccac gttatggttg gagttagcga aacacctgta ctgccattcg
    10561 ttggcaaatt gatggcaaac ttatgaagtg tgaatgttct tacttccgat actccatcag
    10621 gtgtatttgc tttacgtgtc ttaactctcc aataatattt tccgttgtct ttcaggtagt
    10681 ttatgtttgt acttgaaaaa gtgttggcag ttaaatcacg ggtataaatt atttggttga
    10741 agttggcatc ttccgcaact tcgagtgtaa aataatcaac caaatcactg gagttccatt
    10801 taaattcaaa tggtgggaat acggtcgtga cgtcggcagg ataaacaagt tgaggaatta
    10861 tttgggtacc tggagttttt cccataacta acggtggaaa ttcatttcca tatctgtcca
    10921 atatagctac agcaaaagta tttgttgtcg tagaaatatt tgcaggaact gtaaatgaac
    10981 tttgataggt aattccaatc aggtagtttg aaacactaaa gtttccgaca tcattcactt
    11041 tctcattggg tatggcatag actgtgtatc tgagatttgg tccggcatcg ttccatgtaa
    11101 gcgcattacc tgataaatct attcccgaga caagactata ttcaggatgt tgtccccaat
    11161 ttattgccgg tgtcagtgcg ggtttgacaa actgatattt tcggatgtaa tttataaaac
    11221 cggcaatatt ttgaacgggt tttgtaggaa agaaaatgct tcccggcgca ccatttaagt
    11281 cttcctgacg gttattgttt acctgattac caatttcgga agcataaaaa tttgtagctg
    11341 tgggagcata agaaatattt gaattccact gagttgcaat tctttcaatc cccgacatgg
    11401 aagataaagc tgtctgttgc cccgcaactt tcatcatttt tggaacggaa gcgcctccgg
    11461 ctgtcatagc tgaaagtgaa tggctggaat aaaaatgtct tccaaattta tttgctacaa
    11521 aagaccacca ggtacataat tttgtataat catttgctga acctatagtc cagtaaatct
    11581 gtggtgagat gtaatcaatc gttccctgac tcaaccaggc aagagggtct gaaaatatgt
    11641 cgttatattg ccagtcgctt ccaggacatg gagtaactcc gtatttattg gctaccacct
    11701 gactggaagc agctacacca gccggactga tcccaaattt gacgtagggt ttcacacttt
    11761 taatcgtatc acgtactcct ctgaccattt tatttacatt ttgacgtctc cagtcacccc
    11821 ttgacaattt atcgggatta tataatttga actgagcatc gtccatcgca tcggttgtac
    11881 cgctcacata aaaataatca tcaaaaataa ttccgtcgac atcgtaattt ttcacgattt
    11941 ctgctactat tttttttatt tgcagaagca cttccggatg cccgggatta agaatggaat
    12001 tatttgtagc tgagtatgtt agcaaccaat ccggatgtgt ggtgcgataa tctcctggct
    12061 taccagacca ttgattgtta acagattcga aacgatatgg attaatccag gcatgcactt
    12121 caattcctct tttatgtcct tcgtccacta taaatgccag tggatcataa ccagggtcca
    12181 ttcctctgtc agcgactaag tcagatgacc atggttcaaa agatgattta tacattgcat
    12241 cgcatcttga acgtacctga aaacatactg aatttgcgtt cattctcttc aatgaatcca
    12301 atattcctat catttcattt ttctgcatct gaatttctcc ggaattccca gttgatgtta
    12361 tcactgtgct tttcggccaa tcgttacgcc atacagtagt gagccaggta gctcggaatt
    12421 cacgttttgg agactgtgaa aaaccactga aggcaattga tagaattatt gatagtaaga
    12481 gaattttttt catcaaattt tcagattaaa ataattttga ttttgtctta ttcactgcaa
    12541 caccataaac atcgattcct tctataacgt attcaataaa accgcttgta ggattgtaac
    12601 gcataagtcc ggatttaaca gcggcatctc cagaccgaag tccgaaataa acacttccgt
    12661 cattttcgtc caatgctaac tgacaaattg ttatgaattc gcctgcagag ccttctcctt
    12721 tcccggtttc tgttattggt gttatagttt ttccatttgc taatttcaat ctgtatggag
    12781 ttaaatcagc ccgtgctttt attgcattta attgttctaa cgtacatctg taaagccctt
    12841 catatcctgt atcatataat gtgaaataaa taactttgtt tttcgcatcc cacactaaag
    12901 attttggtgt catactctct aaagctatac ctgataatgg taaaacgccg tttcctgtga
    12961 ttggagtttt aaggatatcg gtatctaaga agcggaaaat accatttcca ttataggttt
    13021 tgcaccagta ccaaacatta tttattttcc caaaacaagc attcatagct ccatagctta
    13081 atccctgacc ataataacct aacgttgcgt tttgtacaaa atatggaaaa tctgctctgc
    13141 taaatgttct gtttctttct ttcagcccaa tcgttgagat acctgtattt ctatcagaaa
    13201 aataaagttg tccaccatca atatatccat agtatgggtc gttaaaggct gctccaatat
    13261 tggtcaacat tgtttcaact tttgctccat cctttgacat tacgaagatt cttccatctc
    13321 ccaagttgcc atcttgatca tcgacataag taaattgtct gccacaatca atcacataga
    13381 gagaagtatc actaaataaa atgttcaaag ggtgctgccc ggatttagta cccatatcaa
    13441 aaggatttat tttcattccg gccggtgcat tacttactaa cttcagagcc ataatgtttc
    13501 ctcctttcac cgcataatat agtgtaggaa ctgcctgatt ataagccaca ggaactttta
    13561 taacaccgtc ttccagttga cgtccgccca gttttacctg aagcgttact gtttgtgatc
    13621 ctatattgct aaaagtcaaa tttccgggat ctttaccgtt aaagtctgat atgatagtgc
    13681 ctgccgcatc tttagttcca tcagggaaaa tccaatgata ttcttctgat aacccttccg
    13741 gattaggtaa ttctacattg aatcctaccg aagaagaaat aacttcaatt agtccggttt
    13801 tattcaaaga tacaattggt ttgatatcac ttataaaaat aggttttgtt tggtaaccgg
    13861 cattggctac agtaagttta acagtgtatg tacctgcagt tttatatttg tgtgtcacgg
    13921 ttgaaccggt ggcaattgtt ccgtcaccaa aatcccaggt gcattctccc actgcaacag
    13981 aggtacttac aaactcaatg gtggctccca cgtaatagtc tatttgataa gtaggatcaa
    14041 ttacagagta tttaaaagct atattcggat ttggcaattc ttctatatct ggttcattgt
    14101 ctatgcaaga aaacaccaga aaagctgtga ctaatgtcaa taagtatggt aaaattttca
    14161 tagtcgttgt tttaatttag tgagatttct ttaactattc caagtcctgc tacaccttgt
    14221 ttatcatatg ccttcatgtt tgcatttatt ctgtaagaac gagaatagtc tacgtcataa
    14281 acatttgtgg ttttgttaaa tattatatcc gaaaacggaa tggtagcata tatgtccacg
    14341 agagttttag gtacaggtct gttaccttgt gcatcctttg gaaacacata atcccaacta
    14401 cctgtattat catttttaac tgaatcaata tacattttga aattatcaag taaattcaac
    14461 cctaaataca tcttctgaaa atctttagct ttcattaatt taaataagct atccttaaac
    14521 tgtgtcataa atgcatttcc aaaatacttg accattttat cgttgtcaaa agtagctggt
    14581 ttgctccacg ttaccgatgc taaagtatta cttgtaggaa acgttgattt aaacgtatat
    14641 gcacgcaatg aatcattcca ataattcgga ttatgatcat aggttgctat tttttgtgaa
    14701 actcgttttt ctgacgactt atttgaaata acactatacg taaaagtagt aacccaggga
    14761 cttgagacag attttgattc gtcttcaaga atattatacc aaatctctaa acgttcgata
    14821 ttctcttttg atgtagtgta atactgaaca gtaaaaggaa tatcttcgcc agcttttacc
    14881 gtactgctgg caggttccca gtatacttgc ggtgccattt gccccacaat cacgacatca
    14941 ttcatcatat catgtttatt gcaggcaacg aataagaaaa ttgcggcaat aaagaatatt
    15001 atattgtatc ttgttttcat attattttcg ttattttggg gttatttcca ttctatatcg
    15061 ggatctgcag aagttacgta actattgttt ttatatgccc atatcattct ttttcgcaac
    15121 caatcaggat ttttatatgc gcctaagcgc tccaattctt tgcggttgta tttatcttca
    15181 gtttcagggt catagtttaa acgtgtaatc catgcatttg gtgaaaattt tgcattaaca
    15241 ccaaaattat cgggtttgtc aatccaatat gcagaataca aattaaacgg tcttgtcaat
    15301 gaaaattcct gggtgaaatt tgtaggattt acatttctgg ttccatcgtg gatacgtgtt
    15361 acatgatata cgaaaacgcc atcataggca ataccgttat ttttactact atagttatat
    15421 cttctcatat cagtccattg ctcaggttga agatacatag ccacatattt ttgctgcatt
    15481 aagtcggcaa tagtgaatgt tgcagctcct ggtaatcgta ctaaaaagaa acgatcaata
    15541 aatggtttca tattgggatc gtcaaccttt actcccacgc gtctcatatt tgattttacg
    15601 gcatcgacgg tggtattata agctcccgtt ttatctccac tccagtattg agcttcggct
    15661 tttataaata aaagttcttc ccgggacatc aacattacat atccgttgtt tttcgtataa
    15721 ggatttcctc cctgcagcgc atacagatcc ggatagaatg ttattttcat tgtagtaggc
    15781 attcctatat tattttctat atatcggata gcatttagat ttttatcatc aagacgcggt
    15841 gtcataagtt gctccgccct ggggtccaga gcaaaaccaa ttgtttgtct ggggttcgaa
    15901 taaacactca gcaacgcata tgcaaaaaat ttagaaggaa ttgcatcggt tagaagattt
    15961 tcacgtgctt gcgcccatcc taaattaagt ttgggttgag ccggtcccca cggacagttt
    16021 ttttcggtag agccaccatc ataatgatag agtgcatctg cataagaagg atcattcaac
    16081 gctgcgtcgg cagcatctat tattttttga caattttcaa gagtattttt ccagtttgga
    16141 atcatacgaa gtaaaagacg ggcttttaat gcttttgcat atgcacgcca ttttcctaaa
    16201 tctccaccaa aaagtctgtc ctgtaagcct gaaattgaac ggttattttt attatttatc
    16261 cattcaggat tttcatatag acttattaat tcgtcagctt ctttattcat ccattcatat
    16321 acagattcct gtgtgtcgta tgatggagaa ttggattgat aaacctgaga aagaggaata
    16381 tcaccaaaag catctgtcgt aaacaaggtt gattctaatc tgattgttcg tgcaataagg
    16441 atgtagttcc tcgagtcgat tttctctgca ccttttataa gagcatctgc attaaccccg
    16501 atatcataaa aatggcgtct ccattgcgga tgtctgttca tcgaaaggag ttcccagcct
    16561 gccttataag cgtaagaact ggtttgacta cgtcctccgg ttgtcaacgc ttgtgacaga
    16621 tatgttccat attcagcgtg gtcataacaa atctgtgaag aataaaatac caatacaggc
    16681 agtaactgct caggagagac ctcagatgga gtatctttac ttgtattgat atcaaaatag
    16741 tcctggcagc tattaaaaag gaacacgctg ctaataaaca atgcaaaaag tattttgtaa
    16801 gctttcatag aaaatctgat taaaatgtag ctgaaactgt taaatgaatt cctcttgtag
    16861 gcggtacagg ataataatca atccccattc ctcctgtccc tcctgaacca gtattagcat
    16921 ttatctgagg atcagaacca gtatactttg ttagaaggaa tagattggac ccggttaacg
    16981 agcattttag acttgtgaac tgtgttttgt ttttaatcaa tgatgaaaaa ttataaccga
    17041 tagtcaggta attcaagcgg atataagaac catcttcaat aaaattggaa cttactccgg
    17101 cataatactc tgtaattgtc ttactgtcaa gcactatcgg cgtagtattt tttgtatacg
    17161 taccatcagg ttgctgaact actccatccc atacgacttg tcttccacga tagaactcga
    17221 ggtctttatg ctgaccgtcc gaccacatac cacggcttgt ggcattgtaa atgtcacctc
    17281 ctttacgacc atcaatcaaa aatgcaacat taattccctt aaaataaaac gaattattta
    17341 atcccataag gaaatcgggc tctctgtttc caatcaaaac acttttagtt ggattgattt
    17401 tcggatatcc atctgtattg acaattactt ccccatcttc agtcctcaga taatcttttc
    17461 cagaaatagc agtggtagag ccattcagat aagcagtagg aaacacatcg gtatattggg
    17521 ttccctgaat ttcaacaata tcatccggaa gtttaactac tttacctctg ttaaaaccaa
    17581 aggtcataga ggatttccat gtgaagtccc gatttgtaag aatatcttgt tcccataaaa
    17641 attccacccc gtgattttct actgttcctt cattcctggt ttgtaaaata taaccggaag
    17701 caggtgacac gcgcactgta acaatctgat tatcgacata agttgaataa taagcaaaat
    17761 caaaatgtgt tttattgttg aataaacgtg tttcaagtcc aatctcccat gagtcagaca
    17821 tttctggtct taataaacta gcaaccgatt ttgaagggtc aactccgtat cctccatctg
    17881 gtaaggtcgg atattgggtg tattttcggt caaaaagaaa gggagcaggc gtatcttttc
    17941 ctactcttgc ccagtttcct cttaccttgc cgtaattaaa ccatttgttg cttagtttga
    18001 ataattcgga gaataaaaca ccaccagtga tagatggata aaaataagga ctaaattcct
    18061 gtgataatgt agatgaccag tcccagcgcg aggtagcgct tatggaagcc attcctttgt
    18121 aatcaagcgt tatctgatca aaaataccac cgtttcttct ttgcgtatgt gaaactccaa
    18181 tatcattaac gccttgtatt tcttttacat tttcaaggct ataaattcct ggtactataa
    18241 agtctcttcc gttgatggaa gaagatattc catctcttgt ttttaattca cctcccaaca
    18301 acaaatccat cgaaagttca ctcggaagat ttattttata ggtcagaata cccatcaaag
    18361 ttaaatactg acttcttgaa tttgaatgtg aataatttcc caataaattt ttatctacat
    18421 ttgcataatc agcttgatta aaataaggac ttttatagta attagagtta taatttgcta
    18481 atgcctgagc ataagcatct gtattgtcac catattgatc agcttttgga gcatctggta
    18541 aaattaccga atcgtcaaat cgtgggacag cgtaaccatc gtataaataa ctgttggttt
    18601 caaaatttac ccgaccgata gcttcaaaat tcttaaatgc tttataattc acagctccct
    18661 gcataatagc acgcaggttg tcatttttcc cgtaatccat atatcttgaa aacaaaggac
    18721 ttattggtga acctgatttg agcttttcac taatataccg atatcttact tttccatttt
    18781 ccatatagtt agtgatatca tcattgatag gccagctgta agccgatgaa atacctcctg
    18841 cccctctgta gacatcatta acgatattag ccatggcaga aaaaacaatt tttttactga
    18901 tatcgtaact tcctttagca agaataccca ccttattcaa ataatcgttt acaacaattc
    18961 cttcatcttt tgaataaaaa gcagaagcgt atgctttgac tttctcactt cctccactga
    19021 catttatatc atatttttga taaaaaccgt ctttgaagaa attttttata ttatcgtaac
    19081 ggggttcatc ggatccaagc aggggtcccc aacctccgta accgccgctg gcttccggtt
    19141 tataaaaacc aagagttccg ggaccataaa tagattgttt cgcaggggtt cggattggct
    19201 ggttagtttg aatgctcgcg tttacattag tagttgtttt tccgacaaca ccctgttttg
    19261 tcgtaatcaa aacagcacca tttgcaccac tttgcccgta aagggccgaa gctgccgccc
    19321 cttttaagac agtgatattc tcaatatcat tcgggttgat atcagaaaca cctcctgata
    19381 tggcaactcc atctaaaaca tataagggcg catttgattg agaggggttg atcgatgaaa
    19441 taccacggat aatcaataat gaaccggaat tcggagaacc tccggaattg gtaacattga
    19501 taccggatac tttcccctga agcccattta caaaattaga ctgacgtcct tcagtaagtg
    19561 catctgaatt aacggtctgt acagcaaaat ttaatttctt tttttctgct tttacaccca
    19621 ttgcagttac aaccacttct tccagaaact cggctacagg cttcagtaca atattaacta
    19681 ctgtctgatc acccagctta agttcttgtt tttccatacc aacgtaagaa aaaaccaatg
    19741 tttcattttt agatgctgaa attgaaaagc gaccatcaat attcgttagc gctccccgcg
    19801 tagtaccttt caccgcaacc gacacaccga taacgggcat ttgatcttcc gaagaagtta
    19861 caacgcctgt tataatattc tgggctaaaa caggtgaaaa aaacaataga gaaagtaacg
    19921 ctaaaatgta gaatttcttc atagaactta ttttttgtaa gtttgataat aaatataggt
    19981 tctcctaaca gagaccaaag ttagaaaaaa taatttaaaa agtccagttc ttttttgtgt
    20041 tttttttctt aatgcaaaat ttacaaaatg ttacaaccat ataagtagct ttttatcaca
    20101 aaaaagtaac agtatattaa tgatttacgt tcaaataata aactttttac agttaagcat
    20161 aaatttttat ccgcataact gacatttttt gcatgcctta ttaactaaaa atcacatttt
    20221 aatcaaacac taaatcgtaa tattgaattt atttaattct atttttaaac caaatgttgt
    20281 ttttttagtc aatatgtaat tatttgatag tttttccctt ttgcaggtat tatttttaaa
    20341 tatatttcca aaatgaaaaa agatattttt ctacactctc tttttgccgg attaatattg
    20401 ttgagctcaa tcaactcata cgggcaatcc tcgctttgta ttttcaaaaa tgatgattca
    20461 caaataaatt catggcttat aagtcagata aaaattacat tttcggaaaa caatctattg
    20521 gtttacaacg gaaactccac aacaaacatc ccgttaaata acatcaggaa gattaccttt
    20581 tcctcaacaa acacaaatgt cagcactgaa aatttttcaa acagcttgac gctttatccc
    20641 aatccggtaa gcaacatatt gcatttgaaa aatctgaatt cagaaaatct cactgtcagg
    20701 atttttagca ttgacggaaa agctgtactc acccgtacgc tttcaaccta ccacacggat
    20761 atagatgtga caatactccc gaaaggatta tacattctca aagcaaatga tcaaactgtc
    20821 agatttacaa aaaaatgaaa aaaacaatcc tttccatcct tttaatcgca ggtgtaatca
    20881 gcttatttgc aagtgaatat atttttattt tcaaaaacga tcactcggta acaagcatag
    20941 caatctcagc tatcgacagt ataaagtttt cggacgatca aacacagctt aacatacaca
    21001 aatccgacca gtcgctaatt cattattctg tttcagaact ggacagcatc acatttggaa
    21061 gtatatatac ccctgatgta agcattactt acaacaacag tgttgtaacc atctctaatc
    21121 cataccagta taaaggaata aacatcacca cttccggagc tgatgttatt gtaaattcca
    21181 cacttgctgg ccaagagatc aactatctga tatcgggtgc aacatccgat ggatcgatca
    21241 aaatatacag caataataaa tatacactaa cactcaacgg agcaaccatc acaaataatg
    21301 acggtccagc tataaactta caatcaacaa aaaaatgcac attaacgctt gcatcaggta
    21361 caacaaacac gcttgctgac ggttcatcat acttatcaag taccgaagac caaaaagcct
    21421 cgttattttc cgaaggacaa attatattta aaggaaccgg tacattaaat gtatcaagca
    21481 aatcaaaaca tgcaatttgt tgcgatgatt acattgaaat agaagaagga aatattaatg
    21541 ttacagcggc ttcaaaggat ggaattcatg ccaagggaca ttttaatctt gaaggcggag
    21601 ctgtcactgt tacagcaact ggcgatgctg tggaatgcga agccggaagc atcaaagtat
    21661 cgggaggttc tatttcatct acaatatctt ccgcagatac taaaggattg aaatcagaca
    21721 gcacactact ggtttcgggc ggaacaataa atttcactgt ttcgggaaat caagctaaag
    21781 gtttaaaagc aggacaaaat atgacacttt caggtggaaa tatcaacatt acaacttcgg
    21841 gtgcggcagt tttagttgct tcgggttcgg gttttgatcc atcgtattgt actgctataa
    21901 aatgtgattc atccatctta atttcgggag ctacaataaa tattaccact tcgggagttg
    21961 gaaacaaagc catttctact gataagaatt tagatattac aagtggcaca gtaaaaattg
    22021 taggaacagg tgcaggagca acttatacaa attctctcgg tgtgattgac gcttacggtt
    22081 caagtggaat tgatgctgat ggaaatgtaa gtattcttgg aggaaacatt tcggtttcag
    22141 aatcgggaag cgcagcaaaa ggcatttcta ccgatggaaa tctaaacatt ggcaatgcat
    22201 caaattcgcc cattatttat gtgaaaaata ttggaacaaa acttttgatt tctggaaccg
    22261 caaattacgc aactgccgta tattctgaac caaaaaacat caaaagcgat ggaattatga
    22321 ccatagcaaa cggtacaatt ggactaaatg caacacaacc cggagcaaac accattgatt
    22381 gtgattcgtt gcttaatgtt acaggtggag taattaatga taccatttcg ggtaatcaat
    22441 ccaaaggttt gaaatcatcc aaagcaatga ctttgagtgg tggaacaatt aatgtaaaag
    22501 cgtcgggggc tgttgtttta gaatatacaa cttctacaac tcttcttgca ccatcgtatt
    22561 gctctgcaat caaaagtgat gatgatgttc taatttcggg agcaaacatt acaataaacc
    22621 actcaggagc aggaggcaaa ggtatttctg cggataaaaa tattgaaatg acatcgggtg
    22681 tagtaaatat taccaccaca ggtggaggag caaaatactt atatgcaaca agtacgtatg
    22741 attcgtattc tgcagcagct atcacagccg acgcaaatat ttctattttg aatggaaatc
    22801 ttacaacaag tagttctggt acaggaggaa aaggattaaa agcaaatgga acacttacta
    22861 ttggaagttc cacacaaagc ccaactgtaa atttaaatac aaccggaaca acatttactg
    22921 tgtcgggaag cgacttgtgt catcctaaaa caatagtaac agatggagct attacaattg
    22981 cgaatggaac aaataccata tcatcaactg acgacgcaat tcactccgaa acatccatta
    23041 caataagcgg aggaaataac actattacaa aagctactga aggcattgag agtaaatata
    23101 tttacattac cggaggagaa aacaatataa cagcctcaaa cgatggtctt aacggcacaa
    23161 tgggaactgt ttcaggaggt accgaaagta acgataatag tttaatatcc ataagtggtg
    23221 gcacaacata cgttacaggt gcagatgcca ttgatgcaaa tggtaacttt cttatgacgg
    23281 gaggaattgt attttcaaac ggtccagcct caggagctga agagtttatt gacgcaaacg
    23341 gtactgcaaa aataaacggt ggagtatttg taggttgtag tagtgcggga atgttgaaaa
    23401 catccacttt ctcgtcatca acacaaccgt ttttgtatat gactaccaca ttaaattcat
    23461 ctacaatgta cactgttacc acaggaggga attgggtagc atcgtttaaa cctaaaaacg
    23521 gaggcggagt ttgtctcgta tcagcacctc aaatgaccaa aggcggagct tatgtagttt
    23581 atacaggcgg aacttacacg ggtggaacat ccatcaataa tttttatatg aatggaacgt
    23641 ttacaaatac aggtgcatta ataaaaaaat cagcaacact ttcgactaca gcaactttaa
    23701 atcaaataag tttctaaaaa gcacataaaa aacggctaaa gataaataac tttctaacaa
    23761 tcttcaaacc gcgatttatt gttctatctg taaagatgaa cttttcagat agaacaaacc
    23821 agaagaatca tttaattgat taactttgcg tgactatcag atattacaaa tctattacac
    23881 ttgaataact ttcaacacac accttatatt taatcctgct gaatggaaaa atttcttgag
    23941 atattaaacg aattagatcc tatttcattg gctgaaatgg atagtgtaaa actcctgaac
    24001 cggatggatg ttaaatacat cttccataaa agtgagtttg ctgatatact tacagttgcc
    24061 aatgaattct atcgaactct taccatcaac gaaagacgtt ttgaaaatta cgatagtctt
    24121 tattttgata ccgaagattt caagctgtat aatgcacatc acaacgggag gtcgaaccga
    24181 ttcaaactga ggatgaggac ttatatctat tctgaccttc attttttcga aataaaattc
    24241 aaatcgaatc aaaacagaac cattaaaaag cgtgtacagc ttcccaaaaa aacaaatgag
    24301 ataacgggta acgctctgga attacttcaa agcaaaacca gcatgccggt tgatgatctt
    24361 aaaccagcct tgcgtatcaa atgtaaaaga atcacactgg tagataaatc tttgtctgag
    24421 agggtcaccc ttgattttga catgaaatac aatctgggca atgaccaatg gcacgaatgg
    24481 cctgaactag ttattgctga agtgaaacag gaaaaatccg gacattcacc atttattaaa
    24541 ttcatgtata caaaacacat ccgtcagatt tcattaagca aatactgttt cgggatagca
    24601 aactatatta acacggtacg caaaaataac tttaaaacaa gattacacta tgtggacaaa
    24661 atctgtaaaa atcatgccat ttaaaacatt aagtatcatc cttttcatgc tactgctgat
    24721 gccttcagga actcggtcgc aaccacaaat aagcgctgca gcagccgaag ccgaacaaac
    24781 cgcttctgtt cagtcaaacg atgaatccaa agacgttgtt gacatgttta caattgacac
    24841 aaaaaccgaa aaaaaaacaa aggacaaaat acagttgaat ttcaactttt tctttagttt
    24901 attgattgac ctggcatttc tggctttgat tatcagcctg atatattacc ctaattacaa
    24961 acgactggac acggtattca cgtttgtgct tttcaatgta tccatcttcc tgctcacctt
    25021 cctgctcaac catatcaaaa tatcaatggg tgcagccttt ggattatttg ccgtattctc
    25081 gatgctccgg taccgcactg caggaatcag cgtgaaagat atgacatatc tgttcatatt
    25141 catcaccatg ggattgctca gcggtattca actcgaactt tacgaactgg ttattatttt
    25201 cagtgtacta ttcgtagtgc ttattgtgct cgacacccga attttattga aacgcgaatc
    25261 tacaaaaatt atatatttcg aagatgtgga attacttcac accgatcgtc accccgaact
    25321 gattgaggta ctcaaacagc gaaccggatt aaacatccac cgcatcactg tagaagaaat
    25381 atcatatctg cgcgatttcg gaatgataac catttattat tacgaataat gacaacgaag
    25441 aagaattttc taatattcgg attgttaatc gccatgtccg gagttctgaa tgctcaaaat
    25501 caatatggca catggactag cgccggactc gaaaaaaaag caggcaactg gaatttcgga
    25561 gccgaaacag agcttcgtac cattcattat gtaagactga taaatcgttg gagtctttca
    25621 atgaatgccg aatacaaaat tgcgaaaccg cttaaagttg ggattggtta tgaatttatg
    25681 aatgtactgg acgagaaata cctgaactat cagtttcgca acaggttcaa tgcggatatc
    25741 tcagggaaac aaaaattggg aaattttgca ttttcactcc gtgaacgaat tcaactcacc
    25801 aagaaaaacg acagcaaaag gatagatgag gacggataca atgacactta taagattgat
    25861 cctgcctggg tatggcgaaa taaactgaat gcagactaca atataccaaa atgcaggttc
    25921 acaccgggta tatcggttga aacatattat tcgctcaatg atcccgatgg taataaactg
    25981 gataaactca ggtatatact gtacacggat tataaaataa ataaatacaa taaaatcaaa
    26041 atatttggag ttcttaattc ggaaaaaggt accgatgagg ctgactattc cggcaaatac
    26101 aacctggggc tgagtttttc gcatgaaatt aaataacaaa ccatatgggt aaataatgtg
    26161 tcaaatagct tgtatttaat aaaaccatga aattaaaatc aatttacttt ctctttgctt
    26221 tttcagcgct tatattgtca gcctgcgatc ccgaaaatgt gctgaaaccg gcagaagaga
    26281 ctataaaacc actatacgaa tgggacgaaa cgaaaattca gaccattacg tttcaaaaca
    26341 gcgctgtcag tactacttct tctaaagtag ttattacagg agctactgca acgattaccg
    26401 atggtggcta ctatacagtc agcggtaatt caagcaatgg ccggcttatt gtcgatgcca
    26461 aggatgaaat cgtaaaaatc cgccttgctg gattaactct gagtaacgat acaacttctc
    26521 cctttttcat caaggatgct gaaaaaacca tcgtttttct gaaaagcgga acgacaaaca
    26581 ctttttcgga cggagcaagc tatactcaca cgggtgatcc gaatgcaacc atattcagta
    26641 aagattatat tggatttacc ggcgatggca atctggtggt taatgccaat ttcaacgatg
    26701 ccatttcgac tgatgatatg ctctacatcg acaatggaaa ttttactgta cgttcactgg
    26761 atgatggtat tcgcggtaaa gactcagttg ttgtcaacaa tggaaatatt aacatcatct
    26821 gttccaccgg acatgcattg aagtcggaca atgaactgaa agcggacaaa ggctacctca
    26881 acatcaaagg aggcacactt aacctgtcat cgattgatgg tgatggcatt aatacgacta
    26941 aaagactgat aattgaggga gggacaataa cagcccagtc aaccaatagt caggctctcc
    27001 gctccgactc tgtcgtactt atttccggcg gaatcacaac cgtcaatgct tcgaagaaag
    27061 ggatagaaag cccctacatc actatttcgg gagggacaac caccattaat tcgaataaag
    27121 cgggagtaaa tgccacttac ggacttggcg gatcacaaat tgatggtagc atcctgaaca
    27181 tatcaaacgg tattttgtat gttaactctg taagtggtac cggattggac agcagcgggg
    27241 acatcaagat gtcgggtggt acagttgtgg tacagggtcc ttcggattta agcaaggaag
    27301 gtataaaata taacggtata tttacaataa ctagtggttt tctgattgct tcaggcccac
    27361 agtcaaccat ttcagtaaaa gcaccgagtg ccagttcagt tcaaaacacc gtcagaataa
    27421 cctcaacaac catcggaaca aatcttatag ccatccagga tgcatccggt aatacaattg
    27481 cggctttcaa accaacccga agcgcttatg gtctgctgct ttcttcacct gaattaaaaa
    27541 ctggtacaaa ctattcgata ttcaaaggtg gtacatacgc cggtggaaca acagtcaacg
    27601 gattttcgca aaacggaaca tactcgggag gtacactgtt aaaaactttt acagtgaacg
    27661 ataaaattac aaatgtttca ttctgatatt aaatacacca aatcatcata tttaagaaat
    27721 gccttctaaa aacccgaggg cattttttat taaaaaaaca agtattatta ccgttcttat
    27781 aattatgatt ccggatttca aaaaaatcat gttgtaaaaa tgattttttt gaaatccgat
    27841 ttacggtacg atatttgttt ttttaaaagc aaagcaaagc aattcaactt ataaattcga
    27901 ataaaaaata acagccatga aaaaagaatt atcatttgca ttttttgcag cagctatttt
    27961 tctttccctt tccttgttgt cgggatgtac cacttacaat tatggtacaa ccaatgaaca
    28021 aacctattat gaaaatccgc aatgggctcc ggattattat acaggaaccc gctattatta
    28081 cttcccggat attgaatgtt attatgatct tgccacgcgc gacttcattt ttctaaatcg
    28141 cggacaatgg tacttcactc caaggttaac cgacttctac agcgattttg atttattcaa
    28201 tagctacatt gtcatcgtca acgtcaacgt ttatcagccc tggatgcatc accagtatta
    28261 tgtttcacat tatcccagat attattaccg cgattattat gattacagca atattcccta
    28321 tgtacggggc ttcaatgaaa atgtcaaaag cgccgtttac tggcccgaac aacaacgcta
    28381 cagagcacgt gaatggaacg ataacaattt gcgcaacaac cggagattca aatattcgga
    28441 tgcagaccgt cagatgcaag tcaataccac caaccgtata aacaatcagc gttctgagga
    28501 tgtaaatagc tccagaaaca caggaaccgg tggcagaaga agtaacgaaa aatacacaca
    28561 gccacgcaac accaatactc agaccacaca atctacaaac acgactaccc gtccggcaaa
    28621 tacgacgaat acgaccagca caacaaaccg taatacaaat actgaaagaa caacaaacca
    28681 ggctgtcgac acgaattcaa ggacaactac aacaacacgc tcaaccgctt cagattcaaa
    28741 cactccggtt cgacagtcag gaactgtaac ccgttcgtcg gagagaaccg ccaatacgaa
    28801 ttactatggc aaacccatag gtcagccggt taaagtggaa cgtcggatgc gcagcaccac
    28861 taccgaatca aaggccgagg aaaagccgga cacaaccacc ttaactaata ccaccacacg
    28921 aacaagaagt tcaaggtact aaggtattta tacgatttac tctgaaagcg gacaattatc
    28981 acccgctttc ttttttttca ataggcagta aatcagaaag taacagatgt cattttataa
    29041 tattttacac tatcaaaccc ttataaggaa ctgaaaatca aaatataatt tgtacctttg
    29101 cactcaaatt aaaatatata aaataataaa ctgtaatttt ttatgaatgt agttacaaag
    29161 accatcacac tcggtgatgg tagagaaatt accattgaaa ccggtaaact ggccaaacag
    29221 gccgatggtt cagtggtagt acgtatgggt aacaccatgc ttctggctac cgtttgtgca
    29281 gcaaaagatg ctgttcccgg taccgatttt atgccccttt ccgtagaata caaagagaaa
    29341 tacggtgcag ccggacgttt ccccggtgga ttcacccgtc gtgaaggccg tgcaggtgat
    29401 tacgaaattc tggtttcacg tcttgtagac cgtgcattac gcccactttt ccccgatgat
    29461 tatcatgctg aagttttcgt aaacgtaatg ttgatttccg ccgatggtgt agatatgccc
    29521 gatgcattag ccggacttgc cgcttctgct gctctggccg tttctgatgt tcctttccac
    29581 ggacctatct ctgaagttcg tataggacgt ataaatggtg aatacatagt taacccgact
    29641 tttgaacagt tggaaaatgc cgatatcgac atcattgttg ctgctacact tgacaacata
    29701 atgatggtgg aaggcgaaat gagtgaagtg agcgaagacg atctgctcaa tgccatgaaa
    29761 gtggctcacg aagccatcaa ggtacagtgt caggcacaga ttgaactgat ggaggctgtt
    29821 ggtaaaattg aaaaaagaac ttactgtcac gaagaaaatg acgatgatct gaaaaaggat
    29881 atctgggaaa aaacttacaa taaagctttt gcggtagcaa catcgtgcaa caccgacaaa
    29941 cattcacgtt ctgaaaactt tgaagcaatt gtcgctgaat acatagcagg tattgacccc
    30001 gaagttgccg aagctaaaaa agaactgatt ggccgttact atcacgctgt agagaaagaa
    30061 gccatgcgcc gttccatcct tgacgaaggc aaacgactgg atggccgtaa aactacagaa
    30121 atccgtccaa tttggtcgga agtagattat ctgcccggag cacatggttc tgctgttttt
    30181 acccgcggtg aaactcaatc gcttacaaca gtgactctcg gcaccaaaat ggacgagaaa
    30241 ctcatcgatg aagtgttgca gcaaggtaaa gaccgtttcc tgttacacta taatttcccg
    30301 ccattctcca ccggtgaagc aagaccatca cgtggtgttg gccgtcgcga agttggtcac
    30361 ggaaaccttg cttgccgggc tctgaaatca atgattcctg acggttatcc gtatgttatc
    30421 cggattgtat ccgatatatt agaatctaac ggttcatcct ctatggctac tgtttgtgcc
    30481 ggaacgcttg cgctgatgga tgccggagtt cagatgaaaa aaccggtttc gggtattgcc
    30541 atgggactga tttctgaaaa taaaggtcaa aactatgcaa ttctttccga tatcctgggc
    30601 gacgaagatc acctgggcga tatggacttc aaagttaccg gaacacgcga tggtataacg
    30661 gctactcaga tggacattaa agtggacgga ctgtcatttg aaattctcga aaacgcattg
    30721 aatcaggcta aagccggtcg tatgcatata ctcgataaaa ttcaggaaac ccttaccgaa
    30781 ccacgtcccg acttcaaacc aaatgcacct cgcatcgtag tgatgatgat accgaaagaa
    30841 ctgattggcg ctgtaatcgg ccctggtgga aaaatcatcc agaatatgca ggctgaaacc
    30901 ggtgcggtta tttccattga agaaatcgaa gaccagggaa gaattgaaat tgctgctaca
    30961 aacaaagcat cgattgacgc tgcagttgcc aaaattaaag gcatcgtagc tattcccgaa
    31021 gttggtgaaa cttatccgtc gaaagtaaaa tcaattatgc cctatggcgc gtttgttgaa
    31081 tttatgccgg gtaaagaagg attgcttcac atctccgaac tcgactggaa acgcatcgaa
    31141 acaatggatg aagctggttt gaaagaaggt gatatgatag atgtaaaact gctggatatc
    31201 gatcagaaaa ccggtaagtt caaactttca cgcaaagcat tacttccacg tcctccgcgc
    31261 gaagataaac cacgtgaaga aagatcgcac gacgaaaaac cgcgcgaaca cagaggacct
    31321 cgtcctgaaa gacgtgacta aaaccgaacg gtttaaaaaa gtataaaaaa tcagaggctg
    31381 tctcaaattc aaatttgtga cagcctcttt taattaattt tcggcattga ctaaactttc
    31441 gatccggcta atgtttctga ctcccgttta ttaaactcag aaatcataga atatattact
    31501 tctttcacag ttgaaatccg agttgaaaga taggcattgc ttccggcaaa agcatatccc
    31561 ttttccattt tccctttgta agcattataa agggcaagaa taatacaata gggaactacc
    31621 ttataatcac aggtatgaag gcagttgtaa ggacacaact ttggtcttgt tagtcccagt
    31681 tttatatttt ccagaaattt tccctccaat gcacgaccag gcattcctac cggattttgt
    31741 atgatttcca cgtcattttc aactgctttg acatatgtat ttttgaactt tacgtctgca
    31801 tcacactcct cggttgtgac aaaccgaatc gccacctgca cacctttagc gcccagttgc
    31861 ataatatcgt acatattttc tccaaagtaa atacctccgg ctgctacaac ggggatctcc
    31921 tgattgtatt tttcttcaaa aatggcaact tcacgaacca catccgggat cagtttttcg
    31981 atggagtact ccggatcaga aatctgattt aatttgaaac ccaggtggcc accggctttc
    32041 ggaccttcca ccacgattaa atcgggaaga taatcataga gcgttttcca tttttccagg
    32101 ataattttag ctgcacgcgc cgatgaaacg atgggtgcaa gttttgttgt acagtctgct
    32161 gtgcgatact ttggcaagtc gagtggcagt cctgctccgg caaaaatcac atctgttttt
    32221 tcggcaattg ccgttttcac catatcgctg aaatttgtaa gcgccatcat aatattcacg
    32281 ccgataattc cggatgattt ttctttcgct tttcggattt cttctctcaa cccaacgata
    32341 catgactcca tataatctct cgctttttca ctataaatca gtccaagtcc ggcactggaa
    32401 attacaccaa cacctccttc attcgccact gccgaggcta aacccgacaa agaaatacca
    32461 acaccctgcc cccctgaata atgggtacac gtgctgtcag atttccaatt ttcaatccat
    32521 tcataactaa atattttatt aataaaaaag tttaacaacc ttcactcatt ccagtcaaca
    32581 ccccgatata aaaaacagga gaaagtttac gaaaaataat ccgaaaactt tctcctgtat
    32641 gttttttgta gattaattta tttgatgttc tctgaattat ccgtagataa tttttccgtt
    32701 ttcatcttta aattcatcaa agctcttatt gtattgttcc atagctctgt aactcattgc
    32761 catgctcgaa aatccaccat cgtgatagag attctgcatc gtcaccttac gcgttagatc
    32821 actgaacata accatacaat agtcagcgca atctgccgct gtagcattac caagcggtga
    32881 catcctttcc gaaaaatcca gcaagccatc catgcctttc accccactac ctgctgtggt
    32941 tacggttggc gattgagata ttgtatttac acgtacattg tgttcgcgtc cgtagatata
    33001 accaaaacta cgtgctatgg actcaagcat cgattttgcg tcggccatat cattgtatcc
    33061 aaaaacagtt cgttgagcag ccatatatgt aagcgccacc actgaaccat attcgttgat
    33121 tgcatccatt tttttagcta cctgcaacat tttatggaaa gagatagccg aaatatccag
    33181 cgtggaactc aacatatcat agtcaaggtc atcgtaagta cgttttttac gaacgttggg
    33241 cgacatacct attgaatgca atacaaagtc aatttttccg cccagggctt ccattgattg
    33301 tttgaatact tcttcaagat cagttacact tgtagcatca gccggaataa gtttagcatt
    33361 cagcttttca gccagttcgt tggtggtccc cattcgaacg gcaagaggtg tattggaaag
    33421 tgttattatt gcgccttctt caactgcgcg ttctgccacc ttccaggcta ttgacatatc
    33481 attcaatgct ccgaaaacta tacctctttt tccttttaat aaattgttag ccattttttc
    33541 aaatttaata ttaggataaa acaatctgtt accttgtcat ttattttatc cggttttttt
    33601 atcggcacaa aattactaaa attgtgcatt tttgcaaaat taataaacca ttatcggcaa
    33661 ataaattcat attaaatatg ttcggacata tttcttatac cctcagaaat ttaaaatact
    33721 ctttataatt tcgaacattt caatttcaga ataaacacaa aaacggctgc cagtatgccg
    33781 gacagccgtt tttaaaaatt aactttagtt agaaaattat tcttcttctt tcttcgtagc
    33841 ttcgtattcc ttaagtagtt tatcctgaat ttccatcgga acaagttcgt agctggcaaa
    33901 cttcatggta aacgaagcac gtcctccggt taacgaactc aacgtcgtag aataactgct
    33961 aagttctttc aaaggcactt tagctgaaag tttttcgaaa cccttttcac tgtgcatacc
    34021 cataatgatt gcacgacgac cctgcaggtc actcataaca tcgccaagac gatcggaagg
    34081 agcaaatact tcgacatcgt aaatcggttc gagcaattta ggaccggctt cacggaatgc
    34141 ctgagcaaag gcgttacgac ctgcaagacg gaatgaaatt tcgttcgaat caaccgggtg
    34201 catctttcca tcgtaaacga taactcgtac atcgcgggcg taagaacctg taagaggacc
    34261 ctgttcaatg cggtccatca aacctttcat aatggcaggg atgaaacgcg cgtcaatagc
    34321 accacctaca atactgttga ttacaaccag tttgccgccc cattcgaggt caacggtttg
    34381 cgtgtcacga acacttattt tgtattcctg accattgaag cggaacatat caggagcagg
    34441 cataccttcg tagtaaggtt ccacaatcag gtgaacctca ccaaactgtc cggcaccacc
    34501 cgattgtttt ttatggcgat aatcagctcg tgcaaccttg gtaatggttt cgcggtatgg
    34561 tattttcggt tctttatatt ctatctgaat tttatcgtta ttttcaaccc tccattttaa
    34621 tgtacgcaaa tgaaactcac cctgacccga aagaatagtt tgcttcagtt ctttggactg
    34681 ttcgataatc caggtcggat cttcttcgtg catacgggtc agaatttcgt tcagtttttc
    34741 ttcatccgat tctgatacag ccctgatagc acggcgatat tttggatccg gatatttgat
    34801 ggctttataa tcataatcgc aacctttttc gttcagtgtg ttacctgtac gggtatcttt
    34861 cagttttacc gttgcaccta tattaccggc taccatttca tcaacaacat ttctgatctg
    34921 tcccgaaacg gaatataact gagtgatacg ctctttcgaa ctgcgactga tgttaaccag
    34981 atcatcacct gccttcactt tgccactcat taccttgaag taagaaactt caccgatatg
    35041 aggttctaca gtagttttaa agaagaataa actggttgga cctttggcat cgggtttcac
    35101 ttcaacacct gcagtagtaa ccggagacgg catttggtcg ggtgaaggag ccaccatgct
    35161 caggaaattc atgatacggt gaactgccat gtttttttcg gcagaagcgc agaaaacagg
    35221 gaaaatagaa cggtctatca tacccttcat aattccttcg cgcatttctt cttcagtaag
    35281 tgaaccctgt tcgaagtatt tttccatcag attttcatca ttttcagctg ctgcttccaa
    35341 caatgcttta tacaattctt cagctttatc cttttcgctg gcaggtatat ccatcacgtc
    35401 tggtgctgtt gcacccggtt tccatttata cattttttgt ttgagcacat ctactattgc
    35461 ttcgaaacct tgtccgctgc caatggtgta ttggataggc accactttgc taccgtatgt
    35521 ttctttcaaa ttggcaattg tattatcgta atctgctttt tcatgatcaa actgattaat
    35581 caggaaaacg aggggtttat gcagactttc agtgtatcgg aactgattaa tggtacctac
    35641 ctccactcca ttcacagcat caataagcat cagagccata tcggtcatat tcaatgccga
    35701 aatcactgca cctgcaaagt cgtcggaacc cggacagtcg ataaaattca ttattttatt
    35761 cttccattca tacgaaatca cactggaaaa tacagaatat ccgtattctt tttctacagg
    35821 aaaataatcg gaaacagtgt tttggctatt gattgagcct ctgcgtttta ttactccact
    35881 ctcataaagc atggcttcta cgagagtggt tttcccggcg ccggaacttc ccagcaatga
    35941 aatgttctta atttcattag tttgatatac tttcatattg tattaattta gagagagtta
    36001 attaattaca tttagttaaa ttatgaaatg caatttataa aaacaaaatt aaaacctgca
    36061 ttaaatttat atgttttaca acataatatt aatcctaaat gcgggtataa ctaaagtctg
    36121 ccaaaaagtg taaggtgatt taaatgattt ttatcgattt tatgataaaa gaaaagttta
    36181 acaattggat taaaagacac taccggagcg attaactccg gcagtgcata tctttattca
    36241 tttcatccat tctgtttata gggtcgggtg cggttctact gcttctttgt gattagcagt
    36301 tcttttactt aaggtagttt taaaataatc tacccgatag gacgttcaat tacatagatg
    36361 agaattgact atgtgaaata aattaattag ccccgatatc aacatactga tatcggggct
    36421 aaaacaaagc aaatgtatat cggttattta attttcacaa ccactttgcc ttttatattt
    36481 gccgaagaag ctgctgtttg aagaataaat tctcccggtt caactaccca gctttgggtt
    36541 ttgttgttgt aatatgccaa ttcctgtgtt ttgattttca actccaccgt agtagtttct
    36601 cctgcattca actgtacttt tttaaatgct tttaattctt ttacaggacg gagtacagat
    36661 gcctttggtt gtgaggcata cacctgaact atctccgcac cggcagtttt acctgtgttt
    36721 gacaactgga atgtgagttt aattatatcg tttgcctgat attcggtttt gtcggttgat
    36781 ggtttaccgt acgaaaaagt ggtatagctg agtccgtatc cgaacggaaa ctgaggtgta
    36841 atctttttgg tgtcaaacca acgatagcca accagaatat cttccatgta ttcttgctta
    36901 ttatctttgc ccgggtacga aagcgctccg tacgaatatg cggcattgtc ttccagtttt
    36961 ttggggaaag agaacggcaa cttgcccgaa ggatttactt ctcccgacac aatgttggca
    37021 atggcatttc cagcctcaga acccagatac catgcctgta tcaaagcagg aacctgcggg
    37081 agccagggca tttcaaccgc gttgccgctg caaagtatca ctgtgaggtt tttattgacg
    37141 gtcataagcg tgttgatgac tttgtcctgt tcgtagggaa gtgccatcga ctgacgatca
    37201 tcgccttcgc tgtcctgaaa ccagttttta tttaaacctc ccacaaaaat aaccgcatcg
    37261 gcatttcggg cggtttctac ggctgatgcg agcaatgaat ccgcaatgat tttgctttcc
    37321 ggtttccggt agtccagatc ggggctgtaa ccgcgcgaga aagctatggc ttcggctccg
    37381 tattttgcta taagcccttc gagtggtgaa acttcgtaag ccaccttgag cgatgatgac
    37441 ccaccaccaa ttacgagact tcgaacggca ttgtcgccaa tcactgcaat gcgtttatat
    37501 ttacccaccg ggataggcaa tgtttgtttg tcatttttca ataaaacaat tccctgttca
    37561 gcaattttgc gggcagcttc ggaatgttcg ggactcacaa attttccgaa gggacgattt
    37621 ggagccatgg tggttcggaa aatgagccgc agcaagcgtg taactttctg gttaagttcc
    37681 ttttcgctgt atttacccga ttttattccc tgcagatagg gatctgccag gtaatattct
    37741 gaataaggcg aagttccatc gg
//
LOCUS       DAOPYB010000012        36735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1035_length_36735_cov_9.472983, whole genome shotgun sequence.
ACCESSION   DAOPYB010000012 DAOPYB010000000
VERSION     DAOPYB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 36735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36735
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1035_length_36735_cov_9.472983"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /gene="rpmB"
                     /locus_tag="PKH58_01870"
     CDS             complement(<1..205)
                     /gene="rpmB"
                     /locus_tag="PKH58_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNX87803.1"
                     /translation="MSKICQITGKKAMGGCNVSHSKRHTKRTFDVNLFTKKFYWVEQD
                     MWISLKISAAGLRTINKIGLDAAI"
     gene            complement(392..1624)
                     /locus_tag="PKH58_01875"
     CDS             complement(392..1624)
                     /locus_tag="PKH58_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNX87804.1"
                     /translation="MVEIITIGDEILIGQIVDTNSAWMATELNNAGFQIVQITSVHDD
                     AAHIKKALDEALGRAQVVLITGGLGPTKDDITKQTLCDYFNTRLVFDEKVLQNIRELY
                     HTRQNVMNELTRSQAMVPENSIVIQNRAGSAPITWFEKEGKVVVSMPGVPHEMKKVMS
                     DEVIPRMQKHFKTPVIIHKTVQVYGIPESQLAIKLTDWESSLPDFIKLAYLPNFGIVK
                     LRLSGVSSDALKLEFALNQQIETLNHILGESIFSYEDKPIEKIIFEKLKNSGLTVSIA
                     ESCTGGNIAHRLTLIPDVSECFKGSVVAYHNDVKIKALGVSPDDLMQHGAVSREVAEQ
                     MAEGVRKLTQADLAVSTTGIAGPSGGTEEKPVGTIWIAACSKECMISRCFTFGNLNRE
                     NFIERSTMAALMILNELI"
     gene            complement(1695..2291)
                     /locus_tag="PKH58_01880"
     CDS             complement(1695..2291)
                     /locus_tag="PKH58_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNX87805.1"
                     /translation="MIQINDTIISLELFEERFLCDLASCKGICCIEGDSGAPLEWEEI
                     DILEAILPEISEDLSEVARNLIDKQGVYYIDEDDEPVTSIVNGRECVFTYTDENGVCK
                     CAVEKAYREGKIDFYKPVSCHLYPVRLQKYKEFTAVNIHKWSVCECARSLGKINNVPV
                     YKFLKEPLIRKFGVEWYEQVEFAANELAHSDLSGMDMQ"
     gene            complement(2295..2933)
                     /locus_tag="PKH58_01885"
     CDS             complement(2295..2933)
                     /locus_tag="PKH58_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNX87806.1"
                     /translation="MNLTFINIIDYLGTFAFAISGMRLASAKRFDWFGAYVVGLVTAI
                     GGGTTRDLLLDLTPFWMLQSSYLVVTGFALLFVIFLGKYVIRLHNTFFIFDAIGLGLF
                     VVVGIDKTLELGFPFWVAILMGMITGSVGGVIRDILINEVPLIFRKDIYALACVVGGV
                     FYFICFKLNFSPVVTQTVAAISVILTRILAVKYHISLPVLKAHEDFKKNKED"
     gene            complement(2993..3406)
                     /gene="secG"
                     /locus_tag="PKH58_01890"
     CDS             complement(2993..3406)
                     /gene="secG"
                     /locus_tag="PKH58_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNX87807.1"
                     /translation="MYVFLIILIVIFSLLLTLIVLVQNSKGGGLAAGFQSSNQIMGVR
                     KTTDFLEKATWGLAAAIVVLSIITVGVKSNAPKEVNDSEIKEQSLDLNKQMQQKTATP
                     NFGETAPAQEKQAATATETPKEEPAAQPAAQPEKK"
     gene            complement(3419..4081)
                     /locus_tag="PKH58_01895"
     CDS             complement(3419..4081)
                     /locus_tag="PKH58_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87808.1"
                     /translation="MTYDDFIDKIQQPDMLTGEQVPVLKELVNQYPYFSIGRWLYLKA
                     LYNSNSIYYGNELKRTAVQSSSRRNLYFFIHPEELKTDGKKERSGNSSGSYFDMLGAI
                     DNKGENNQNTLRLLAERLKAAREMLQPGNSEKETITEISDSLNVEISPDLSIEKKDQI
                     TKKLSGKSPEELELEAKKYIKAKKYKEALEILKELNFNNPKKSIYFADQIRFLEKIIQ
                     NL"
     gene            complement(4098..4622)
                     /locus_tag="PKH58_01900"
     CDS             complement(4098..4622)
                     /locus_tag="PKH58_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNX87809.1"
                     /translation="MKKINKLIVFLSLFSVAFILNGCAVTYGFKSGTLDYTKIKSISI
                     SDFPNNADLVNPTLSQTFVETMKDKYTRSTRLQMLSRGGDMHIEGEIVGYQLTSMAIS
                     AESSFASETKLTLTINVRFTNNKKPEDDFETKYSASQMFDSNLMLNDVEAELVKIMVD
                     EIVDKIYNDTVAKW"
     gene            complement(4633..5904)
                     /locus_tag="PKH58_01905"
     CDS             complement(4633..5904)
                     /locus_tag="PKH58_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX87810.1"
                     /translation="MKPNELQSVKHRFGIIGNSELLNRAIDIAVQVAPTDLSVLITGE
                     SGVGKENFPQIIHNYSHRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGATADRKGY
                     FEVADGGTIFLDEVGELPLSTQVRLLRVLETGEFIKVGSSKTQKTNVRVVAATNLNIT
                     QAIRDGRFREDLYYRLNTVPVFIPPLRDRKEDIPLLFRKFAADFAEKYKMPPIRLSDD
                     ARQMIMNYYWNGNVRQLKNITEQISVIEQQREITPAILRSYLPAEDIERYPALFERER
                     SEHKTFNNEREILYQVLFDMKKDMNDLKKIVNDLMLGQPVHVQPVDTGYETEIYHHDD
                     TAGQNPTQSYTAGIPNQKKFSHVDDVETFQDVEDYQDENEVKYSLADMECELIKKSLE
                     KNNGKRKLVAKELEISERTLYRKIKDYGIDS"
     gene            6153..8111
                     /locus_tag="PKH58_01910"
     CDS             6153..8111
                     /locus_tag="PKH58_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNX87811.1"
                     /translation="MKSEHKVPFGLMGLFLAIGIVYGDIGTSPLYVMKAILGGLPEGG
                     KANPEYIYGAISCIIWTLTLQTTVKYVLITLRADNHGEGGILSLYALIRKNYRWAYLI
                     AAIGASTLLADGVITPAITVISAVEGLNTLQPSLPILPITIAILIVLFFIQPLGTSRL
                     GKSFGGIMFLWFTMLGILGVYGLLFYPHVFAAFNPVYAVRLLQASPNAMIILGAVFLC
                     TTGAEALYSDLGHCGLNNIRISWIYVKVTLILNYLGQGAWIITHPNMLVEGVNPFFSL
                     MPGWFSLIGVAMSTFAAIIASQALISGSFTIISEAISLDLWPNIHIKYPTEIKGQMYI
                     PLVNFTLMVLCIAVVVFFTSSSNMEAAYGLAITITMMMTTALLFIYLYNSHKPLWFSI
                     PVTLFFITLEGGFFTANMLKFIHGGYVTIIIAGGLFFIMYVWYNGRRIKNRSVSYIPI
                     AKYISQIEKISNDKEVPKFATHLVYVTRAKYPDEMESKIIQSLINKQPKRADTYWFVY
                     LTRSDDPFEFNYEVKTFIPGKIFRLNIRAGFKMGIHIDTYVRRICKEMEKKGEVDLTS
                     RYPSLKETNIEGDWRFVVVERIVRNLDLPTKSKAILSIYYGIKKFTTSDTQILDLDPS
                     VVTVEYVPLKNLRPAPRKDQPYFSYEEE"
     gene            8283..9386
                     /gene="ald"
                     /locus_tag="PKH58_01915"
     CDS             8283..9386
                     /gene="ald"
                     /locus_tag="PKH58_01915"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNX87812.1"
                     /translation="MKIGIPKEIKNNESRVSLTPSGANTLVLHGHTVFVQKNAGVNSG
                     FSDDRYIKAGAQILPDAESVYDQADMIIKVKEPIESEYKLIRPNQLVFTYFHFASDEK
                     LTHAMIESKAVCLAYETVELNKTLPLLLPMSEVAGRMSVQEGAKYLEKPKGGKGILLG
                     GVPGVNPANVLVIGGGVVGQNAALMAAGLGARVTICDISLPRLRYLSEIMPSNVVTLM
                     SSKYNIEELLPSVDLVIGAVLIPGAKAPYVITKEMLKLMEPGSVMVDVAIDQGGCFET
                     SHPTTHADATYKVDEIVHYCVANIPGAVPFTSTLALTNATLPYAVMLADMGWEAACAK
                     HPDLRLGLNIINGKVVYKAVADTFGLPYEELKF"
     gene            complement(9488..11266)
                     /locus_tag="PKH58_01920"
     CDS             complement(9488..11266)
                     /locus_tag="PKH58_01920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX87813.1"
                     /translation="MTFKELNLKDGILSAIEELGYEQPMPVQEQVIPFMLTQSADLVA
                     LAQTGTGKTAAFGLPILNMIDVNRNQVQALVLAPTRELCIQISNDLKGYSKNLNGLRI
                     VPVYGGEDIRVQLRQLDQAPQIIVATPGRLIDLIERGKIKLQNIHFLVLDEADEMLNM
                     GFKEDIEKILEQTPESRRTTLFSATMPAEIQRIARQYMHDYEEITIGSRNAGNENVEH
                     IYYVSQARQRYLVLKRIVDLNPDIYGIVFCRTRQETKEVADKLMHDGYNADALHGDLS
                     QAQRDTVMQKFRIRNLQLLVATDVAARGLDVSDLTHVINYNLPDDVEIYTHRSGRTGR
                     ANKTGVSVSIIHSKEKFKIKDIERMLKRKFEQQQIPNGLEVCKKQLFFMIDRMQNVEV
                     NEEQIAPYMSQIMKQMEGLPKEEIIKRFVSLEFNRFLDYYKNAVDLNIAEHISRERDG
                     RNDRSGEEGGKQRKKSGVRVRLKINLGSREGMNPKRILGIINDVTEDKSINIGDIEIT
                     NKFTFFDVFEDQQDKVIRAFEHAEDMEVAVAKGSKSANDNREKSKRDYSGKDRNRSSA
                     GNYKNEEKPWRNRDRKSSGSDKRKRW"
     gene            11674..13320
                     /locus_tag="PKH58_01925"
     CDS             11674..13320
                     /locus_tag="PKH58_01925"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNX87814.1"
                     /translation="MTKSPLQIARAHYQPKLPVALQNYVAIKEGKATESVADQDDIKK
                     LFPNTYGMPLLTFEPGEKKNYPVVNVGVILSGGQAPGGHNVISGLFDGLKKLNPENKL
                     YGFLDGPIGLVEHKYTELTEKVVADYRNTGGFDIIGSGRTKLEEEWQFDKGAEICKEL
                     GIQAIVIIGGDDSNTNACVLAEYYKQKDTGIQVIGCPKTIDGDLKNEMIETSFGFDTA
                     CKVYSELIGNIQRDANSAKKYWHFIRLMGRSASHIALECALQSQPNVCLISEEVEANN
                     QTLDDIVNNIVKVIVNRGNAGLNFGTVLIPEGLIEFIPAMKTLIAELNDMLADNDDFN
                     AMNTDDERREYVKGMLTPESCNIYRSLPKGIAKQLTLDRDPHGNIQVSLIETEKLLIE
                     MVGKRLAQMKAEGKYKGKFSAINHFFGYEGRCAIPSNFDADYTYSLGYTAAILISEGK
                     TGYMSSVRNLTEPATEWIAGGVPITMMMNMERRSGKMKPVIQKALVRLDGEPFKFFAK
                     YREKWEDAQPDYIYPGPIQYFGPTEVCDQPTRTLLLEKNK"
     gene            13620..14444
                     /locus_tag="PKH58_01930"
     CDS             13620..14444
                     /locus_tag="PKH58_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX87815.1"
                     /translation="MLRYIIRKKITIIFLLINVMLFSSCITSRKTNYLQEPSVFIPSY
                     DDKTGYEEYKLTPSDKLYIRMYSTEKETNQHFNGNNQSMTTMMSGGGSSDYTDLYTYT
                     VKNDGTIKVPIVEEHIYVAGKNIRETKKIIEEALKPYLLEKFVVDVKIIGRYFSVIGG
                     GSTGKFPIVREKMNIFQALALAGDLNTYSDRSKIKILRETNGTTQIKVFDIRSKDIIN
                     SEFYYIQPNDVIYIQNVNEQFFSVSTFGAALSTIFSTFSFGLFFYNLFSPTTPASN"
     gene            14451..15236
                     /locus_tag="PKH58_01935"
     CDS             14451..15236
                     /locus_tag="PKH58_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX87816.1"
                     /translation="MLKSVYKYKYCLLNPVQLLLLTVVVFILSSCYTPSNTLLLQDAR
                     SLPKYSKADYLDYKIQVNDELMFRLITSSKEFDELISAGSNASSQQSNSYRVYPDGTI
                     DLPFMDSIPVASMTFPEATKVIENRFKELIPDAEIILTLANKTYTVIGETGTGIFPVY
                     KDRLTIYQAIAQSGDILQSGDKRHVKILRDTKDGPAIMEFDIRPTSVIDSKYYYIYPN
                     DIIYIRKQSSSFYKVNNYGSFLGLIMSSVSLFINVFYYTKLSK"
     gene            15279..17669
                     /locus_tag="PKH58_01940"
     CDS             15279..17669
                     /locus_tag="PKH58_01940"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNX87817.1"
                     /translation="MDEYYYSKQKFNDEEESGFDIKEWIALFLHYWYLFVVFGVLALG
                     LAYLKNRSLIESFQSSGTLIIEETRYSNGSQALMQGFGVQAGYSNVDNQIVMLTSYDL
                     INRVVDSLPQLNIDYISKGRFKTRNLYSNSPIQINSDYVDPNVYGALFKISLKTDGSF
                     IITDEDGTVDKSLKIQGRYGEPLQHNLFFITVFPNNQQYTNQDLYFRFRSKESLVSDF
                     ISRLDLKFVLEGSSVLNVSLISETPERDIDFINKLCQTFLAENLERKNDAATKTMRFI
                     DEQLDAVSKSLSVSEGAMTEFRRSNQIVDLPTHSTEVLGKATQYDEKRAELKLRETYL
                     NYLTNYLRTNLASGAVVAPSSLGLNEPMLMALVQQFNELISERNKISERNPLYAKYDR
                     EIDNLKLSINEVVKNMRASMNIEEDDLNKKIAGVQKEIDALPNKEMEMISIERKYRVD
                     DNYYTFFLQKRAEAAIQKASNSPDNNILDKARVITQINQDKKSRTTLIYLLIGLLIPA
                     IFVILKELLNNTIRNMKDVEKNGAFTLLGAIRHTHSTDPILAGIRPRSSFTEMFRVIR
                     TRIEFIVQRKSKIAIMATSAESGDGKTYFCTNLASVYALTGLKTLLMDMDIRKPSINN
                     RLGITEKNGVTNYLIGEFELNDLILRKDGIPFDILLAGTVPPNPGELIRSDKLKDMFA
                     QLRTMYDYIIVDSSPIGLVADAYSLAPLMDTNLMVVRSEKTNKGFFKKLNIQLKADNI
                     SNMYTVINDVKPDGTSYSKYTSNNSYGYGYGYGYGTKEGKKKGNSYTHYYEDDNDF"
     gene            complement(17750..18082)
                     /locus_tag="PKH58_01945"
     CDS             complement(17750..18082)
                     /locus_tag="PKH58_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87818.1"
                     /translation="MNKKILSIAQITGAVLVFGGAFLKLLEYGTPTTYIFSAGALILI
                     VVQFIHTLQARKEEKRIQRLNRLLLIATAVLGLSAYMMFAKPYSESWVVMVLLYAVLS
                     VFLSFRTK"
     gene            complement(18079..18390)
                     /locus_tag="PKH58_01950"
     CDS             complement(18079..18390)
                     /locus_tag="PKH58_01950"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNX87819.1"
                     /translation="MSFLQNKGSAFRHIILNKYVITLVVFFVFLIFFDDHNLINRWQT
                     SRRIRGIEKEINFYQSEIDKNRKKMNEMQTSDKSLEKYAREQYYLKKDSEDVFIIREE
                     E"
     gene            complement(18478..19248)
                     /locus_tag="PKH58_01955"
     CDS             complement(18478..19248)
                     /locus_tag="PKH58_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87820.1"
                     /translation="MLTALIHNIHTADNLLKSAARLAADLKKDFGIICYADNLQQAES
                     VQNNIRNYLSENNFVDVQLFVKVETKPLLADECENNDTSFLIIQLMDNSRKNIQKQLD
                     MCRELRIPYVFFQEEFPVFDLRKVMLPIGFLPEELEKAQFASAFGRFCGSEVLMLLAN
                     DYGSKAAMNAERMKELFYRFEFVYRQEKAKTDSFKVEKEAVFRSVREGYGMVIISASR
                     DYGLDDLIFGPKELHLIRKSSVPLLIVNPRGDLYALCD"
     gene            complement(19341..21800)
                     /locus_tag="PKH58_01960"
     CDS             complement(19341..21800)
                     /locus_tag="PKH58_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HNX87821.1"
                     /translation="MKLSKIAHLLGTDDNNIPEVDIHWLLTDSRSLSFPSGTLFFALK
                     TSRNDGHRYIAELYRQNLRYFVVSEMLPDFETLTDAVFIHMDDTLQALQTLAAAHRAE
                     FSIPVIGITGSNGKTVVKEWLYQMLHTDFRIVRSPRSYNSQIGVPLSVWSLSDSSELA
                     IFEAGISRTGEMQKLQPIIRPTIGIFTNLGDAHQEGFTGLKQKCDEKMKLFIDSEVLI
                     YNSDNKLVDISIAQAGLKARLFSWGKKADTTVQVLKTEKTETGTILYLKYQEKKFELN
                     INFSDDASIENAMQCICTMLYLNYKSVEINKRLLNLETVAMRLEVKEGNHDCLLINDT
                     YNSDLNSLSIALSFLNQQSTKKNLSRTLILSDILQSGHSSEELYELVARLVQSKNIHR
                     MIGVGEVISSYADRFQTEEKYFFRTTDDLLKSPLMREFSKEIILLKGSRSFHFEDILA
                     KLELIVHETTLDVDLNALVDNLNYFRSKLRPETKIMCMVKAFAYGSGSVEVARTLQNN
                     RCDYLAVAVGDEGAELRREGIRVPIVVMNPEKGAFGTIFDNKLEPEIYSFRLLKLFMV
                     AAEKLALTDYPIHIKIDTGMHRLGFNPEDMSELTTLLANQNQLKVRSVFSHLVGSDSP
                     QFDDFTRQQLELFTRCADELSKPFSHKIMRHILNSAGIERFPEFQFDMVRLGIGHYGI
                     TALPNVKLATVCTLKTIILQIRDVKAGDTVGYSRKGKITRDSRIGVIPIGYADGFDRH
                     LGNGTGEVWVNGCRAKVVGNVCMDAVMIDLTGIPASEGDEVEIFGKNISIDEVAEKLQ
                     TIPYEILTSVSRRVKRVYFIS"
     gene            complement(22046..22819)
                     /locus_tag="PKH58_01965"
     CDS             complement(22046..22819)
                     /locus_tag="PKH58_01965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX87822.1"
                     /translation="MNDDTKSIHEFDMQLICEYFSSLERQGPGSPEVTLRALSFIDNL
                     TAESRIADIGCGTGGQTMMLAQHAPGTITGIDLFPDFIDLFNANAAKLNLQERVTGKV
                     GSMDQLDFQPGELDMIWSEGAIYNIGFERGVNEWRKFLKPGGYIAVTEASWFTDYRPT
                     EIDDFWNDAYPEIDTIPAKMLQLQNAGYIPVAEFILPEKCWTENFYVPQKKAQEVFLE
                     KYPGNTAAEELVANERHEAHLYDKYKEYYGYVFYIGKKI"
     gene            complement(23127..23615)
                     /gene="tpx"
                     /locus_tag="PKH58_01970"
     CDS             complement(23127..23615)
                     /gene="tpx"
                     /locus_tag="PKH58_01970"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNX87823.1"
                     /translation="MATTNFKGNPVNTNGELPAVGTKAPDFKLVKSDLSEVTLKDFAG
                     KKVVLNIFPSIDTGVCAMSVRQFNKWVSEKENTVVLCVSKDLPFAASRFCGAEGLDKV
                     VTASDFRYNTFATDYGVLQTDGPLQGLCARAVVVVDENGVVTYTELVPEITTEPSYNL
                     PI"
     gene            23784..24983
                     /locus_tag="PKH58_01975"
     CDS             23784..24983
                     /locus_tag="PKH58_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87824.1"
                     /translation="MNKNLTYILFFAAIPALFSCKDKTEYRVGSDFEIYVQRFEQEAA
                     TRNRTFNFKSSGLIIEFSNLKEGVAGLCNYEKPIRIEVDKAYWDSLGLQNGTDQMREE
                     LIFHEMGHGILNRTHTNSVLINDEWKSMMCGGNAISGRTWNLNYHGERRAYYLDELFN
                     ESAPIPAFAAESFNADTTNFIEVYRDEFTSTYSTKWKLGQTNNGKATIENSMLKYESQ
                     SSVNLIVLIATPVNVLSDFSFECTLQYTGADISKYGIVYGTYADENQQTDASGASLEY
                     LLINNDRKATIGNRSWFSYFTQVTRDQIVPKSLNKIKVVKKCSMMYFFINGQYAYRSE
                     MVNSAAGYNFGFSVPPKSTLYVDNFRIAQSSGSMNAKFRMKKAETSQFEIREIKTELG
                     TRTIKDK"
     gene            25018..26709
                     /locus_tag="PKH58_01980"
     CDS             25018..26709
                     /locus_tag="PKH58_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HNX87825.1"
                     /translation="MAKFVIYQVLPRLFGNYNPTRKHNGTIYENGCGKFNNFTPKAFE
                     AIKELGVTHIWYTGIIEHATKTDYSSFGIRRDHPGVVKGNAGSPYAIKDYYDVDPDLA
                     DDVPNRMAEFEDLIRRTHDAGLKAIIDFVPNHTARLYYSDVKPEGVQDLGETDNPKLA
                     FSPQNNYYYTPETAFTPSFDTGGYTEMPAKATGNDQFTAFPSEYDWYETVKLNYGVNY
                     VEGMQKHFDPIPDTWLKMVDILCYWAAKEVDGFRVDMAEMVPVEFWGWAIPFVKAINP
                     DIIFIAEVYQPAEYRNYICNGKFDYLYDKVGLYEKLRGIISRNEPARDITAQWQNLSG
                     IEDRMLNFLENHDEQRIASGFFCGSGVYAQPAMIVSATLTQSPVMIYMGQELGELGMD
                     KEGFSGVDGKTTIFDYWGVSSIQAWANGGKFDGVLLTEEQKELRAFYQKLLQVSMNEK
                     AITEGVMYDLEFANFGNPNFNTHEQFAYFRKHKKELLLCVVNFQDKNFGMEINIPSEA
                     FQFLNLVEDEFYSSENLLNSDEDFPVQLLSSQSPYRVFMPAWCGKILKLKKKKIS"
     gene            26810..28090
                     /gene="eno"
                     /locus_tag="PKH58_01985"
     CDS             26810..28090
                     /gene="eno"
                     /locus_tag="PKH58_01985"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNX87826.1"
                     /translation="MSKIEKIVAREVLDSRGNPTVEVDVTLESGVVGRASVPSGASTG
                     ENEAIELRDGDKKRYLGKGVLKAVENVNKVIAPALIGECALQQRKIDKAMIALDGTKT
                     KSNLGANAILGVSLAVAKAAANYLDMPLYRYIGGTNTFTLPVPMMNIINGGSHSDAPI
                     AFQEFMIRPVGACCFREGLRMGAEVFHSLKKVLHDKNLSTAVGDEGGFAPNLAGGTEE
                     ALESILTAIRNAGYEPGKDVMIGLDCASSEFFKDGIYDYTKFEGANAKKRTSAEQVDY
                     LAELVANYPIDSIEDGMAEGDWDGWKLLTDKLGKKIQLVGDDLFVTNVEYLKKGIDMG
                     CANSILIKVNQIGSLTETLDAIEMAHRAGYTSVTSHRSGETEDATIADIAVATNSGQI
                     KTGSLSRSDRMAKYNQLLRIEEELGDLAAYGWKK"
     gene            28547..29662
                     /locus_tag="PKH58_01990"
     CDS             28547..29662
                     /locus_tag="PKH58_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNX87827.1"
                     /translation="MITIQEAKTKKEMKKFIMFPFELYKNDKTWVPPLIFDEWNTFNP
                     KKNPSFEFCEAAFFLALKENKVVGRIAAIINHKANEAWNEKRTRFGWIAFIDDPEVSK
                     ALLDTAEKWGAEKGMQGIHGPLGFTDLDPEGMQVEGFENNATITSAHNYPYFPVHMEQ
                     HGFRKSVDWVQYKFNASQPIPEKVGRINKLIAQKYKVNTVVPKTKKDALKYVPGFFKG
                     VNEGFKNLYGYSQLSDKQIKYYTDAYFGFAKPELLCFLTDENDEVVGFGICFPSLSKA
                     FQKAKGRLFPFGFIHILKALRNYDEIDLYFNGVVPEWQHKGIHSLYYVAMNEVAIERG
                     VKWAYSTGQLETNVNAVGIWDNYDKQPLFRTRCYIKD"
     gene            complement(29750..30505)
                     /locus_tag="PKH58_01995"
     CDS             complement(29750..30505)
                     /locus_tag="PKH58_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87828.1"
                     /translation="MRLKIYKLIFAVLFTLLCTGFVQAQEPQKNDTTTATLKTTTQVE
                     PINRTFYHWEIPILEPVNIDSIRQQKKDFEGYKLQIDSLQDVLKNNRKQISKYQDQAD
                     SEAKLVKKERSNLKQKVKFFKEDEKLLKTEKKLRQKELKMLLKERKDFIKTSKGLSGN
                     EVDFRNTKFDEREKRVADANANWANKMDNLRKSQAQIENEQKKLEDRAREVDARQTEL
                     KTYEEALQLKARQISIEKKQANLEIKRAKIQLK"
     gene            30832..31671
                     /gene="dapF"
                     /locus_tag="PKH58_02000"
     CDS             30832..31671
                     /gene="dapF"
                     /locus_tag="PKH58_02000"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNX87829.1"
                     /translation="MKFTKMHGTGNDYIYVNGFENIIENPGEIAIRLSDRHKGIGSDG
                     LVLILPSETCDFRMRMFNSDGSEAEMCGNASRCVGKYVYDKGLTTKTEVTLETLAGVK
                     VLKLFVNNDNKVEKVTVDMGEPILDSKLIPTTFDESPVINKVVSFGKYTDYQVTCVSM
                     GNPHAIIFTENVSELDLPKIGPIVEHATYFPKRTNTEFVEVISESVLKMRVWERGAGE
                     TMACGTGACATAVAAVLNGVADRKTTIELLGGNLEIEWREADNHVYLTGDAVTVFEGE
                     VEL"
     gene            31708..32931
                     /locus_tag="PKH58_02005"
     CDS             31708..32931
                     /locus_tag="PKH58_02005"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNX87830.1"
                     /translation="MAYINDNFIKIPATYLFSEIAKRVAQHKTENPEVDIIRMGIGDV
                     TLPLPDASVDAMITAANEQRKSETFRGYGPEQGYDFLRNSIVENDFKSRGIDIEADEI
                     FVSDGAKSDTGNIGDIFGLQNRVAITDPSYPVYVDTNAMGGRAGEPSASGQWTNLVYL
                     PCNTANNFVPELPNEKVDIIYLCYPNNPTGTTLTKEQLTVWVNYARENNAIILFDAAY
                     EAYITEENVPHSIFEIEGAKDVAIEFRSFSKTAGFTGTRCGYTVVPKSVTALSENGEK
                     VSLNKLWLRRQTTKFNGTSYVVQRAAEATYSSEGKKQVRALVNYYMENARIIRESLKA
                     AGLTVYGGVNAPYIWTKTPDGISSWEYFDYLLKEKNIVCTPGAGFGPAGEGFVRFSAF
                     GSRENTLKAMKRLEK"
     gene            33465..34115
                     /locus_tag="PKH58_02010"
     CDS             33465..34115
                     /locus_tag="PKH58_02010"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87831.1"
                     /translation="MKKVKILLIVMLTSAAFLSIEAQGVSINADLVSTYVWRGTKLSG
                     ASIQPLLQFTKGGFCIGSWGSAGFDGYLEMDLFTRYTFNFGLSAGLTDYYFPGTNYFD
                     YSKDTGSHGYEVNLGYAVHGFSIGANYMLNEAGGAKTAGGDKYFELGYSLKNISIFAG
                     AGDGWHTPTGDFGIVNLGLSATKELSVSETLRIPVKLSAILNPTTKQYYLTAGITL"
     gene            34357..34719
                     /locus_tag="PKH58_02015"
     CDS             34357..34719
                     /locus_tag="PKH58_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNX87832.1"
                     /translation="MKKIEAVIRKSRFEDVKEALNEAGIEWFSYWDITGLGKSTEDQV
                     VRGQIFKSNYIQRRMLSIVVRDVNLEKTVNAILKSARTGEMGDGKIFVYDIEETYRIR
                     TGESGPGALYNKENSVNL"
     gene            34794..36107
                     /locus_tag="PKH58_02020"
     CDS             34794..36107
                     /locus_tag="PKH58_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNX87833.1"
                     /translation="MSSQTLVATTPTINSGDTAWMIVATALVLFMSIPALALFYGGLV
                     RRKNVLNVFMQCLIIVAVISIEWVVCGYSLSFGSSHGFLTPFIGGFDWAFLNNIHISD
                     LSPYFISHSQVAADGTSTGTIPHLVFVMYQCMFAVITPALIIGAFAERINFKGFLIFT
                     ILWSLLIYNPVAHWVWSADGWLFKLGALDFAGGTVVHVNAGIAAIITAIMLGKRRDYD
                     GHALPPHNITYVVMGAAMLWVGWFGFNAGSGLAADGLAANAFMVTHIAAAAAALTWAI
                     LDWFNDKKPTIVGTSTGAVAGLVAITPAAGFVGVGGALAIGVVVSLICFVMVARVKPK
                     LGYDDSLDAFGVHGIGGMIGAIMTGLLATPLIQPAYSGAFYGNWHQLVVQLIAVGATI
                     IYSGIGTFLIFKVTESIIAIRANDKHEAIGLDETQHGETAYTSFE"
BASE COUNT        10965 a         7880 c         7162 g        10728 t
ORIGIN      
        1 ttatggctgc atccagacca attttattga tggtacgtaa tccggcagcc gaaatcttca
       61 ggctgatcca catatcctgt tctacccaat agaatttttt ggtgaacaaa ttcacatcaa
      121 aagtacgttt ggtatgacgt tttgagtgtg atacattgca tccacccatt gcttttttac
      181 cggtaatttg acaaatcttt gacatcgctg tatctcttta atttttttct atattttttc
      241 tcaaaaacag tgtgcaaaag tattgatttt ttttctttca cacaagtttt ttctgtttat
      301 atttcaaatt ttctgcccaa aaataaattc aggatattca ataaacttat tatcagacaa
      361 ataaacacca aattgatttc aaaaataaat ttcaaataag ctcgttgagt atcattaatg
      421 ctgccatcgt actccgctca ataaagttct cgcggttaag gtttccgaaa gtgaaacaac
      481 ggcttatcat acactctttt gaacatgcag ctatccagat tgtgcctaca ggtttttctt
      541 ctgtccctcc ggatgggccg gcaataccgg ttgttgacac ggcaaggtct gcctgagtta
      601 attttctcac accttctgcc atttgttcag ccacttccct gctcactgct ccatgctgca
      661 tcagatcatc gggcgagaca cccagtgctt taattttcac atcattgtga taagctacga
      721 ccgaaccttt gaagcattcc gaaacgtccg gaatcaatgt aagccggtga gctatatttc
      781 ctcctgtaca actttcggct atcgaaactg tcagtcctga atttttcagt ttttcaaaaa
      841 ttattttttc tatcggttta tcttcgtatg aaaaaatact ttcacccaaa atgtgattta
      901 atgtttctat ctgctgattc agggcaaatt caagcttcaa agcatcagac gagacaccgc
      961 tcagacgcaa ttttacaata ccaaagttgg gcaaataagc cagtttaata aaatccggca
     1021 atgagctttc ccagtcagtg agtttgatgg caagctgaga ttccggaatg ccataaacct
     1081 gaacagtttt atgtattatt acaggcgttt taaagtgttt ctgcattcgg ggtatgacct
     1141 catcactcat tacttttttc atttcatgag gtacacctgg catagaaact accactttac
     1201 cttctttttc gaaccaggta atcggagctg aaccggccct gttctggatg acaatgctgt
     1261 tttccggaac cattgcctgc gatcgggtca gttcattcat cacattctga cgtgtgtgat
     1321 agagttcccg aatattctga agcaccttct catcgaatac cagtctggta ttaaaataat
     1381 cgcaaagtgt ctgttttgta atatcatcct tggtaggtcc gagacccccg gttatcagca
     1441 ctacctgtgc acgtccaagg gcttcatcga gcgctttttt gatatgagca gcatcatcat
     1501 gaacagatgt aatctgtaca atctgaaatc cggcattatt gagctctgta gccatccacg
     1561 ctgaattggt gtccacaatc tgcccaatca ggatttcatc accaatcgta ataatttcga
     1621 ccatataatt ttcctgatat ttcctgaagc aggagtttat aatctttctt taatatcttt
     1681 tttttgccag cctgtcattg catatccata ccggacaaat cagagtgagc cagctcattt
     1741 gcagcaaatt ccacctgttc gtaccattcg accccgaatt tacgtatcag gggttctttc
     1801 agaaatttgt aaacaggcac attatttatt tttccgagtg agcgggcaca ttcgcacaca
     1861 ctccatttat gaatgtttac agctgtaaat tccttgtatt tttgcagcct aaccggatac
     1921 aaatgacatg aaaccggctt gtaaaaatca attttacctt cgcggtacgc tttctcaaca
     1981 gcacatttac agacaccatt ttcgtccgta taagtaaaca cacattcacg tccgttgact
     2041 atcgaagtaa caggttcatc atcttcatca atataatata cgccttgttt atcaatcaga
     2101 ttccgcgcca cttccgataa gtcctcactg atttctggca agattgcttc caggatatca
     2161 atttcttccc attccagtgg agctcccgaa tcgccttcaa tacaacaaat tcctttgcag
     2221 gatgccagat cacagagaaa tctttcctca aaaagttcaa ggcttataat tgtatcattt
     2281 atttgaatca tcgtctagtc ttctttgttt tttttaaaat cttcatgggc tttcaatact
     2341 ggtaaactaa tatgatattt cacagcaagt attctggtta aaataaccga aatggcagcg
     2401 acagtctgag ttacaacggg agaaaaattg agtttgaaac atatgaagta aaaaacgcca
     2461 cccacgacac atgccagcgc atatatatct ttccggaaaa taagcggaac ctcattaata
     2521 agaatatcac gtatcacacc gcccaccgaa ccggtaatca tgcccataag aattgctacc
     2581 cagaatggga aacccagttc aagcgtttta tcaatcccta caaccacaaa aagccccaaa
     2641 ccgatagcat caaaaataaa aaatgtattg tggagccgta tgacatattt tcctaaaaaa
     2701 atcacaaaca gtagcgcgaa gccggttacg accaggtaag aagactgtaa catccagaaa
     2761 ggagtaagat cgagcagtaa atcgcgggtg gttccgcctc caatggcagt aacaagacca
     2821 acaacatacg cgccgaacca gtcaaaccgc ttggcagaag caagtcgcat accactgatt
     2881 gcaaatgcaa aagtacctaa atagtcaatt atattaataa aagtcagatt catagaacaa
     2941 aaaaagcgta tcaaaaagta tcttgatacg cttatattat acttaattcg tattacttct
     3001 tttctggttg tgctgccggt tgtgctgccg gttcttcttt aggagtttca gtagcagtag
     3061 cagcctgttt ttcctgtgca ggagctgttt caccaaaatt cggtgttgct gttttttgct
     3121 gcatttgttt gttcagatcc agagattgtt ctttgatttc agaatcattg acttcttttg
     3181 gtgcatttga tttaacgcct acagtgataa tcgataaaac tacgattgca gcggctaatc
     3241 cccaggttgc tttttcaaga aaatcggtag tttttcgaac acccataatc tgattggacg
     3301 attgaaaacc tgcagccaga cctccgcctt tggaattctg caccaacacg atcagagtaa
     3361 gtaataacga gaaaatgaca attaaaatga ttaaaaaaac gtacataatg agtttatttt
     3421 ataagttctg tataattttt tctaaaaatc gaatttggtc tgcaaagtaa atactttttt
     3481 tcggattatt aaaatttaat tctttcaata tttctaatgc ttccttatat ttttttgctt
     3541 taatatattt ttttgcttcc agttcgagtt cttccggact tttgccggat aatttcttgg
     3601 ttatctgatc ttttttttcg atcgacaaat caggagatat ttcaacattt agactatccg
     3661 aaatttccgt tatcgtttct ttttctgagt taccgggttg cagcatttcg cgtgcagctt
     3721 tcaggcgttc agccagcaat cgcagcgtat tttggttatt ctcacccttg ttatcaatcg
     3781 caccgagcat atcgaagtaa ctgcctgaag aattccctga tctttctttt tttccatccg
     3841 ttttcaattc ttctgggtgt ataaaaaaat acagatttct gcgacttgaa gactgtactg
     3901 cagtacgttt caattcattc ccgtaataaa tactgttaga attatatagt gctttgaggt
     3961 acagccatcg tccgatacta aaatacggat actgatttac aagttccttt aaaaccggaa
     4021 cctgttcacc agtcaacata tcaggctgct gtattttatc tatgaaatcg tcgtaagtca
     4081 ttatccgatc agaatattta ccacttggca actgtgtcgt tgtaaatttt atctacaatt
     4141 tcgtccacca ttatttttac cagttcagct tccacatcat ttagcatcag attactgtca
     4201 aacatctgag aagctgagta ttttgtttca aaatcatctt caggcttttt attgttggta
     4261 aaacgaacat ttatagtaag tgtaagttta gtttctgacg caaatgagga ttctgcactg
     4321 atagccattg aggttagctg gtaaccgaca atctcaccct cgatatgcat atcaccacca
     4381 cgcgacagca tctgcaaacg ggtggatcgg gtatatttat ctttcatggt ttccacaaaa
     4441 gtctgcgaca aggtgggatt aaccaggtca gcattattcg ggaagtcgga aatggaaata
     4501 cttttaattt tggtgtaatc taatgttccg ctcttaaaac cgtatgtcac agcacaacca
     4561 ttaagaataa aagcaacaga aaacaaactt aaaaaaacta tcagtttatt tatttttttc
     4621 attttaatta tatcagctat caataccata atctttaatt ttccggtaca aagttcgttc
     4681 ggaaatttcc aattctttcg caaccagctt tcgtttgccg ttattttttt ccagcgactt
     4741 ttttataagt tcgcactcca tatcggccag cgagtatttg acctcatttt catcctgata
     4801 atcttccaca tcctgaaacg tttccacatc atcgacatgg ctgaattttt tctgattggg
     4861 gataccagct gtgtaggact gcgtcggatt ttgaccggct gtatcatcat gatgataaat
     4921 ttccgtttca taaccagtat cgacaggctg cacatgaacc ggctgtccga gcatgaggtc
     4981 gttaactatt tttttcaggt cgttcatgtc ttttttcata tcaaaaagaa cctgatagag
     5041 tatttcacgt tcgttgttga aagttttatg ttccgatctt tcacgctcaa acaatgccgg
     5101 ataacgctcg atatcttctg caggcaaata cgaacgaagg atagcgggtg taatttcccg
     5161 ttgttgttcg atgacagata tttgttcggt aatgtttttc aactgacgga catttccatt
     5221 ccagtaataa ttcataatca tctgtcgggc atcgtcagac aaacgaattg ggggcatttt
     5281 gtatttttcg gcaaaatcgg ctgcgaactt tctgaaaagc aagggaatat cttctttacg
     5341 gtctcttagt ggcgggataa agaccggcac agtattcagt ctgtaatata aatcttcgcg
     5401 gaaacgtccg tcacgtattg cctgagtgat gttcaggtta gtggcagcca ctactctcac
     5461 gttagttttt tgtgttttgg acgatcctac ctttataaat tcacctgttt cgagtacacg
     5521 taaaagtctg acttgcgtag aaagaggcaa ttcaccaact tcatccagaa aaatagtacc
     5581 tccgtctgct acttcaaaat atcctttccg gtcggcggtt gccccggtaa atgatccttt
     5641 ctcatgtccg aaaagctccg aatcgattgt cccttcggga atagcaccac agtttactgc
     5701 aatgtaaggc ccgtgtttac ggtgactgta gttgtgtatt atttgtggaa aattttcttt
     5761 acccactccg ctttcgccgg ttatcaatac agacaaatcg gtgggtgcca cctgaacagc
     5821 tatatctata gcccgattta gcaattcaga atttccgatt ataccaaacc ggtgtttaac
     5881 agattgtaat tcgttaggtt tcatatccaa ttcaacatct tatattatca atcatttttt
     5941 gaaaaattaa ctgacaaaat gacaaaaaca gatgacaaaa ttacaaaagt tttcccaaaa
     6001 cctttctgat tataggttaa aaatagtttt cgttacagaa cttcacaaac tgaattatag
     6061 tttcaaccag tatattctca aaatttcaaa tcaattcagc attattagaa attatttcaa
     6121 tacatttgca cgttttttta aaatgaaatg atatgaaaag tgagcataaa gtgccatttg
     6181 gtctgatggg gctttttctg gcaatcggga ttgtgtacgg tgatatcgga acttcgccgt
     6241 tgtatgtaat gaaagcaatc ctcggaggat tgcccgaagg cgggaaagcc aatccagaat
     6301 acatttacgg agcgatctcc tgtattatct ggaccctcac gttgcagaca acggttaaat
     6361 atgtacttat cactttgcgt gccgacaacc atggtgaagg aggaatactt tcgttatatg
     6421 ctcttattcg aaaaaactac cggtgggcat atctcattgc agcaataggc gcctcaaccc
     6481 tgttagccga cggggtcatc actcctgcca ttacggttat ttcggctgtc gaaggactta
     6541 acaccctcca accttcactc cccattcttc caataaccat cgccattctg attgttctgt
     6601 tcttcataca accgctcggc acatcgcgat tgggcaaatc attcggaggc attatgtttc
     6661 tgtggttcac catgctgggc atactgggag tatatggcct gctgttctat ccgcatgttt
     6721 ttgctgcttt taatcccgtt tatgctgtca gattgcttca ggcatctccc aatgcgatga
     6781 ttatattggg agctgtattc ctttgtacta ccggtgcaga ggctctttac tcggatctcg
     6841 gacattgcgg actgaacaat atccgaattt cctggatata tgtaaaagta actttaattc
     6901 tgaattatct gggacaggga gcctggataa tcacgcatcc aaatatgttg gttgagggag
     6961 taaatccttt tttcagcctg atgcccgggt ggttttcgct tatcggagta gccatgtcaa
     7021 cttttgcagc aataattgcc agtcaggctt taatcagtgg ttcattcacc atcatcagcg
     7081 aagccatttc gctggactta tggccgaata tacacattaa atatcctacc gaaatcaaag
     7141 gtcaaatgta tatacctctt gttaatttta ccctgatggt attgtgcata gcggtagttg
     7201 ttttcttcac atcttcttca aatatggaag ccgcatacgg attggctatt accatcacta
     7261 tgatgatgac tacagcactt ttattcatct atctgtataa ttctcataaa ccactttggt
     7321 tcagcattcc cgtaaccttg ttctttatca cacttgaagg tggtttcttt acagccaata
     7381 tgctgaaatt catacatggc gggtatgtga caattattat tgcaggcggt ctgttcttta
     7441 tcatgtacgt atggtataat ggcaggagaa ttaaaaacag aagcgtttcg tatattccta
     7501 ttgcaaaata tatttctcaa atagagaaaa tcagcaacga taaagaagtg ccaaaatttg
     7561 ccactcactt agtttacgtg acacgtgcca aatatccgga tgaaatggaa tcgaagatta
     7621 tccagtcact catcaacaaa caaccaaaac gtgctgatac atattggttt gtatatctca
     7681 cccgaagcga cgatccattt gaattcaact atgaagtaaa aacgttcatt ccgggtaaga
     7741 tattcaggct taatatccgt gccggtttta aaatgggaat tcatatcgac acctatgtgc
     7801 gccgaatctg taaggaaatg gagaaaaaag gtgaagtcga tttaaccagc cgatatcctt
     7861 cactcaaaga aacaaacatc gaaggtgact ggagatttgt ggttgttgaa cgtattgtgc
     7921 gaaacctgga tttgccaact aaatcaaaag ctatactatc aatatattat ggtataaaaa
     7981 aattcacaac atccgacacc cagatactcg acctggatcc atcggttgta acggtagaat
     8041 atgttccgct taaaaatctg agaccggcac cccgcaagga tcagccctat ttcagctacg
     8101 aagaagaata acagtcattc cccagcaata aaatattata catttagtca acagtttagc
     8161 tctaattgaa gatttctaaa gaatataatt ttaattttta agaaaatatt tttgatttac
     8221 atgacaattt gtcaaaaaac tatcgtaatt ttatagcgta aaattcatta actacaagaa
     8281 ccatgaaaat aggaattcca aaagaaatca aaaacaatga aagccgggtt tcattaaccc
     8341 cttcaggtgc caacacatta gtacttcacg gtcatacggt atttgtacaa aaaaatgccg
     8401 gtgtcaacag tggattttct gacgaccgct acatcaaagc aggcgctcag attttacctg
     8461 acgcagaaag tgtttacgat caggcagata tgattattaa agtaaaagaa ccgattgaga
     8521 gtgaatataa attaatccgt cccaatcagc tggtatttac ttattttcat tttgcatccg
     8581 atgaaaaact tacccatgca atgatcgaaa gtaaagctgt atgtctggct tacgaaacag
     8641 tagaattaaa caagactttg cctttgttac tcccgatgtc ggaagttgcc ggacgtatgt
     8701 cggtgcagga aggggccaaa tacctcgaaa aacccaaagg aggtaaaggt attctgctgg
     8761 gtggcgttcc gggcgtaaat cctgcgaatg ttctggttat cggcggaggt gttgtgggtc
     8821 agaatgcagc tctgatggct gcaggactgg gtgcccgcgt tactatctgc gacatatcct
     8881 taccacgtct gcgttatttg agcgaaatta tgccgtcaaa tgttgtaacg cttatgtcga
     8941 gcaaatacaa tatcgaagaa ttgttgcctt cagttgatct ggtgatcgga gcagtgctca
     9001 taccgggagc aaaagcacct tatgtaatta ctaaagagat gcttaaacta atggaaccgg
     9061 gttctgttat ggttgacgtt gctatcgatc agggtggttg ctttgaaacc tcacacccaa
     9121 ccacacatgc tgatgctacc tataaagtag atgaaattgt tcattattgc gtagccaata
     9181 tacccggagc tgttccgttt acctcaacac tggctctgac caatgccacg cttccttatg
     9241 ctgttatgct ggcagatatg ggctgggaag ctgcatgcgc aaaacatcct gaccttcgtc
     9301 tgggtctcaa cattattaac gggaaagtag tgtacaaagc agttgcagac acattcggac
     9361 ttccttacga agaactgaaa ttctaacact catttaccgg tttagccgga gattataaaa
     9421 acacaaaaag cttattcatt gtgaataagc tttttgtgtt tttaaatctg agaagtcgtc
     9481 aaaccgacta ccatctcttt cttttatcgc tacccgaact tttacggtcg cggtttctcc
     9541 agggtttttc ctcgttttta taattacctg cagaggatct gtttctgtct tttcccgaat
     9601 aatcccgttt ggatttttcg cgattgtcat ttgccgattt tgagcctttt gcaactgcta
     9661 cttccatatc ttcggcatgt tcaaaagcac ggattacctt atcttgctga tcttcaaaaa
     9721 catcgaagaa cgtaaattta ttggtaattt caatatcacc gatattgatg gatttgtctt
     9781 cggtaacatc atttattatt ccgagaattc tttttgggtt catgccttca cggctcccaa
     9841 ggttaatttt cagacgtacg cgtactcccg attttttccg ttgcttacca ccttcctctc
     9901 cggagcggtc atttcttccg tcccgttcac gggaaatatg ttccgcaata ttcagatcga
     9961 cagcattttt ataataatcc aggaaacggt tgaattccag ggaaacaaat ctcttaataa
    10021 tttcctcttt gggcagtcct tccatttgtt tcataatctg gctcatataa ggtgcaatct
    10081 gctcttcatt aacttccaca ttttgcatgc ggtcaatcat gaagaaaagt tgttttttgc
    10141 agacttccag cccgttagga atttgttgtt gttcgaactt gcgtttcagc attcgttcaa
    10201 tatccttgat tttgaatttt tctttggaat gtatgataga taccgacaca ccggttttgt
    10261 tagcacgtcc ggtacgaccg ctccggtgtg tatagatttc cacatcatcg ggcaaattgt
    10321 aattaataac atgtgtcaga tcgctcacat ccagtccacg cgcagccaca tcggttgcca
    10381 ccaacaactg gagatttcga atgcgaaatt tctgcatcac cgtatctcgc tgtgcctgag
    10441 acaaatcacc gtgcagcgca tcggcattat acccatcgtg catcagtttg tcagctacct
    10501 cctttgtttc ctgacgggta cggcagaata caattccgta aatatccgga tttaggtcaa
    10561 caatacgttt gagcaccagg taacgctgac gagcctgaga cacataataa atatgttcaa
    10621 cattttcgtt tccagcgtta cgtgaaccta ttgttatttc ttcataatca tgcatatact
    10681 gacgggcaat gcgctgaatt tcagcaggca ttgttgccga gaacagagtt gtacggcgac
    10741 tttcaggcgt ttgctccagt attttttcga tatcttcttt gaaacccata ttcaacattt
    10801 cgtcagcttc gtcgagcacc agaaagtgga tattctgcag tttgatttta cctctttcga
    10861 tcagatcaat caatcgtccg ggagtggcaa caataatctg cggagcctga tccagctgac
    10921 gtaattgtac acgaatatct tcgcctccgt aaaccggcac aatcctcaaa ccattcagat
    10981 ttttcgaata acctttcaga tcgtttgaaa tctggataca aagttcgcgc gttggggcaa
    11041 gcacgagtgc ctgtacctga ttgcggttta cgtcaatcat attgaggatt ggtaacccga
    11101 aagcggcagt ttttccggtt ccggtttgag ccagcgctac caaatcggcc gactgagtaa
    11161 gcatgaaagg tattacctgt tcctgtacgg gcataggttg ctcgtatccg agttcttcga
    11221 tagccgaaag aatcccgtct tttaaattca attctttaaa tgtcattttt tgttttttag
    11281 tacacgtctg gagtagtcgt ccgaagcccg atgaaagtaa aaagcactaa aaaacagatt
    11341 cgtgcttttt taatttttta ctttcaacct catgttagaa taaaaaagaa aaaagtttcc
    11401 gattggaaaa cgtgtacctt aaagatatcc atctgtcaga ccgacagcaa aattcagatt
    11461 tttacccgca acgcgcagtt gtttttaaat cgggcgcaaa ggtacgaaaa atatcccgaa
    11521 tagcaataca agtatgcaaa gttgttattt tgtcaaaaca gaccttataa ttctttcaaa
    11581 tattaattta ttttcctaat tttgcagtga ttttcaataa aataccaaaa tactgaatat
    11641 caataaaata aaaaatacaa atctatgaaa ccaatgacta aaagcccgct tcagatagcg
    11701 cgtgctcatt atcagccaaa acttccggtt gcacttcaga attacgtagc aataaaagaa
    11761 ggaaaagcta ccgaatcggt tgcagatcag gatgatatta agaaattatt tccaaatacc
    11821 tacggaatgc ctttacttac atttgaacca ggtgagaaaa aaaattaccc ggtggttaac
    11881 gtgggtgtta tactttcagg tggtcaggct cccggtggtc acaatgtaat cagcggactt
    11941 tttgacggac ttaaaaaatt aaatccagaa aacaaactct atggatttct tgatggcccc
    12001 attggtctgg tagaacacaa atacaccgaa ttaacagaaa aagtggttgc cgattatcgt
    12061 aataccggcg gtttcgacat catcggttcc ggccgtacca aactggaaga agaatggcag
    12121 tttgacaagg gagcagaaat atgcaaagag cttggaattc aagccatcgt aatcattggc
    12181 ggcgacgatt caaatacgaa tgcatgtgta ctcgccgagt attacaaaca aaaagatact
    12241 ggcattcagg tgattggctg tccaaaaacc attgacggtg acctgaaaaa cgaaatgatt
    12301 gagacctcat tcggatttga taccgcctgt aaagtttatt ccgagctaat cggtaacatt
    12361 cagcgcgatg ctaactcggc caagaaatac tggcatttta tccgtctcat gggtcgatcg
    12421 gcttcacaca ttgcactgga atgcgcccta caaagtcagc ccaacgtttg tctgatttcg
    12481 gaagaggttg aagcaaataa tcagaccctt gatgatatcg taaacaacat cgtcaaagtg
    12541 attgtaaacc gcggaaatgc cggcttgaat ttcggtaccg tacttatccc cgaaggcctg
    12601 attgaattta tccctgcgat gaaaacgctg attgccgagc tcaacgatat gctggccgac
    12661 aacgacgact ttaacgcgat gaataccgac gacgaacgcc gcgaatatgt aaagggaatg
    12721 cttacacctg aaagctgcaa catataccgc agcctcccga aaggcattgc aaaacaactc
    12781 acgctggatc gtgaccctca tggtaacatt caggtttctc tcatcgaaac ggaaaaattg
    12841 ctcattgaaa tggtaggaaa acgtctggct cagatgaaag ctgaaggcaa gtacaaagga
    12901 aaattctcag caatcaacca tttcttcgga tacgaaggcc gctgtgccat accttcaaat
    12961 ttcgatgctg attatactta ctcattgggt tataccgctg cgatcctcat ttctgagggc
    13021 aaaactggct atatgtcctc agtacgcaat cttactgaac ctgccacaga atggattgcc
    13081 ggtggtgtgc caatcaccat gatgatgaat atggaacgcc ggagtggaaa aatgaaacca
    13141 gttatacaga aagcactggt tcgtctggat ggagaacctt tcaaattctt cgcgaaatat
    13201 cgcgaaaaat gggaagacgc acagccggat tatatttatc cgggcccaat ccagtatttc
    13261 ggtccgacag aagtttgcga ccagcctact cgtacgcttt tgcttgaaaa aaataaataa
    13321 gtgataattc aaaccttcaa actccgcatt atataacaga atataatgcg gagttttttt
    13381 ctaaatatat cgtagaggaa gtttctcctg taaaagaaac agatatatac tcagtgtgag
    13441 cttattatca gacataaatc acaaaatact tttataaaaa gcgttaaatt ctttgataac
    13501 agaaaatcct tgtctattca aattataaca taattttaca ttataaagtt atcataatag
    13561 catatattcc aacctatttc aatatctttg caaataaaat cagttaaaat cagttttcaa
    13621 tgttaagata tatcatcaga aagaaaatta ccatcatatt cttactgata aatgtaatgt
    13681 tattttcttc ctgtatcaca tccaggaaaa ccaactattt acaagaacct tccgtgttta
    13741 tacccagtta tgacgacaag acgggatacg aagaatacaa acttaccccc agcgataagt
    13801 tatatataag aatgtattcc acggaaaagg aaaccaatca gcatttcaac gggaataatc
    13861 agagtatgac aaccatgatg tcaggtggtg gttcgtcgga ttacaccgat ttgtatactt
    13921 atacggttaa aaatgatggc accatcaaag ttcccatagt tgaggagcat atctatgtgg
    13981 ccgggaaaaa tatccgtgaa acaaaaaaaa taattgaaga agcgctgaag ccctacttac
    14041 ttgagaaatt tgtcgttgat gtaaaaatca tcggtcgtta tttcagtgtg attggtggtg
    14101 gttctacagg caaatttcca attgtcagag aaaagatgaa tatttttcag gcactggcac
    14161 tggcaggtga tttaaacacc tacagtgacc gaagcaaaat caaaatatta agagaaacca
    14221 acggcacgac acaaataaaa gtatttgata tcagaagcaa ggatatcatc aactccgagt
    14281 tttactacat ccagccgaac gatgtaatct atattcaaaa cgtcaatgaa cagtttttct
    14341 ctgtatcaac atttggcgct gctctttcaa cgattttttc tactttttct tttggtttgt
    14401 ttttttacaa cttattttct cctactactc ctgcttcaaa ttaaccgggt atgctaaaat
    14461 cagtatataa atataaatat tgcctgttaa atccggtaca gcttctgtta ctgactgtag
    14521 tggttttcat attaagttcc tgctatacac ccagcaacac tttactgttg caagatgcaa
    14581 gaagtcttcc aaaatattca aaagcagatt atctggatta taaaattcag gtaaatgatg
    14641 aattgatgtt cagactcatt acctccagca aagagtttga tgagctgatc tccgcgggca
    14701 gcaatgcttc atcacagcaa tccaactctt accgggtcta tcccgatggc accatcgacc
    14761 taccgtttat ggactccata cctgtggcaa gtatgacctt tccggaagct accaaagtta
    14821 ttgagaaccg tttcaaggaa ttaattccgg atgctgaaat catccttacc ctggcaaaca
    14881 aaacctacac ggtaatcggt gaaaccggca caggaatttt cccggtttat aaggaccgtc
    14941 tgactatcta tcaggcaatc gcgcagtctg gcgatattct gcagagtggt gacaaaaggc
    15001 atgtgaagat tctgagggac accaaagatg gtcctgccat tatggaattt gatatcagac
    15061 ctacatcggt tatcgattct aaatattatt atatctatcc caacgatata atttatattc
    15121 gaaaacaatc atcaagtttt tataaagtaa acaactacgg ctcattctta gggctgatta
    15181 tgtcatcagt atcattattt ataaacgttt tttattatac aaaattaagt aaatagccgg
    15241 taagctatcg ttttaaatac ctaaaaaatt cccgaattat ggacgagtat tattacagca
    15301 agcaaaagtt caacgatgaa gaagaatctg gttttgatat aaaagaatgg attgccttat
    15361 ttttgcatta ctggtattta tttgtagttt ttggtgtgct ggctctgggt cttgcctatc
    15421 tcaaaaacag atcattgatc gaaagttttc agtctagcgg tacgctgatc attgaagaaa
    15481 caagatattc aaacggttct caggccttga tgcagggttt tggtgttcag gctggttaca
    15541 gcaatgttga taaccagata gttatgctca cttcttacga ccttattaac cgtgttgtgg
    15601 acagtttacc tcaattgaat atcgattaca tttcgaaggg aaggttcaaa acaagaaatc
    15661 tttatagcaa ttcacctata caaatcaatt ccgactatgt tgatccgaac gtatatggag
    15721 cgctcttcaa aataagcctg aaaaccgatg gcagttttat cattaccgat gaagacggaa
    15781 ctgtggataa aagtctgaaa atacagggca gatatggtga acccttacaa cacaacctgt
    15841 ttttcataac ggttttccca aataaccaac aatatacaaa tcaggatttg tacttccggt
    15901 tccgcagtaa ggagtcgctt gtatctgact ttatttcacg cctggatcta aaatttgttc
    15961 tcgaaggttc ctcagtactc aatgtctcgc ttatcagcga aactcccgaa cgggatattg
    16021 atttcatcaa caaactttgc caaactttcc ttgctgagaa tctggaacgg aaaaatgatg
    16081 ctgccaccaa aacaatgaga ttcatcgacg aacaactcga tgccgtttcg aaatctcttt
    16141 cggtatcgga aggagctatg accgaattcc gtcgctcaaa ccagattgtc gatttgccaa
    16201 cccattcgac cgaagtactg ggaaaagcaa cacagtatga tgaaaaacgt gctgagctga
    16261 aacttcgtga aacctatctg aattatctga ccaactatct gaggaccaat ctggcttcgg
    16321 gcgctgtagt ggctccttcg agtctgggac tgaacgagcc tatgcttatg gcgctggttc
    16381 aacagtttaa cgaattgatt tccgaacgaa ataaaatcag cgaacgaaat cctctatatg
    16441 ctaaatatga ccgtgaaatt gacaacctga aactatccat caatgaagta gtgaaaaata
    16501 tgcgggcttc catgaatatc gaagaggatg accttaacaa aaaaatagcc ggagtacaga
    16561 aagaaattga tgcacttccg aataaagaaa tggaaatgat ttcaatcgag cggaaatacc
    16621 gcgttgatga taattactat accttcttcc tgcaaaaacg tgccgaagcc gctattcaaa
    16681 aagcttccaa cagccctgac aataatattc tggacaaagc ccgtgttatt acgcaaatca
    16741 atcaggataa aaagtccaga acaacattga tttatctgct gatcggtctg ctgattccgg
    16801 ccatattcgt tatattgaaa gaattgctca ataatactat ccgcaatatg aaagatgtgg
    16861 aaaaaaacgg tgcctttacc ctgctcggag ctatcagaca tacgcacagt accgatccga
    16921 tactagcagg aataaggcct cgttcatcgt ttactgaaat gttccgtgta atccgaacac
    16981 gtatcgaatt tattgtacaa cgaaaatcaa aaattgctat tatggcaact tcagccgaat
    17041 cgggtgacgg aaaaacctac ttctgtacta acctggcatc ggtatatgca ctcaccggac
    17101 tgaaaacgct gctgatggat atggatatac gtaagcccag tatcaacaac cgactgggta
    17161 ttaccgaaaa aaacggagtt accaattatc tgattggtga gtttgagctg aacgatctga
    17221 tactcagaaa agatggtatt ccgtttgaca ttctgctcgc gggtactgta cctccaaatc
    17281 cgggcgaatt aatccgttct gataaactga aagatatgtt tgcccaactg cgcaccatgt
    17341 acgattacat tattgtagac tccagcccca taggtttggt tgccgatgcc tattcgctgg
    17401 ctccgcttat ggacaccaac ctgatggtcg ttcgcagtga aaaaacaaac aaaggatttt
    17461 tcaagaaact gaacatccag cttaaagcag acaatatcag caacatgtac accgtaatca
    17521 acgatgtgaa acccgatgga acttcatact ctaaatatac cagcaataat agttatggat
    17581 atggatatgg atatggatat ggcactaagg aaggcaagaa aaaaggaaat tcctacaccc
    17641 attactacga agacgacaac gatttctgat ccggagttat ctctataaaa aaataaagcg
    17701 ggcattcaat caatctgatc tgaatgcccg ctgtgcaaat atatgatttc tattttgttc
    17761 tgaaggaaag aaatacagaa agcaccgcat atagaaggac catcacgacc catgactcgg
    17821 aatatggttt tgcaaacatc atgtatgctg acagaccgag tactgccgta gctattaata
    17881 aaagccggtt aaggcgctgt atgcgttttt cctccttacg tgcctgtaat gtatggataa
    17941 actgaacaac aattaaaatc aaagcgcctg ctgagaaaat ataagttgtg ggtgttccat
    18001 attcaaggag tttgagaaaa gctccgccaa aaaccaaaac tgctcctgta atctgagcta
    18061 tgctgagtat ctttttattc attcttcttc tcgtattata aacacatctt cactgtcttt
    18121 tttcagataa tactgctcac gtgcgtattt ctcaagactc ttatcacttg tctgcatttc
    18181 attcatcttt tttcggtttt tatcaatctc cgactgataa aaattgattt ctttttcgat
    18241 accccttatt cttctggagg tttgccagcg gttaatcaga ttatggtcat cgaaaaaaat
    18301 cagaaataca aaaaacacaa caagtgttat cacatattta tttaaaatga tgtgacgaaa
    18361 tgctgatcct ttgttttgaa gaaatgacat agttaatgta tctgataagt gtacaaagat
    18421 agattttttt gaaatggaaa tcggaatttg aaatgttaaa aaaataaagt tttcggatca
    18481 atcgcacaaa gcatacaggt cgcctctcgg atttacaatt aacaaaggaa cggatgactt
    18541 tcggatcaga tgcaattctt taggaccaaa aatgaggtca tcaagcccat aatcgcggga
    18601 agcggatata ataaccatcc cgtatccttc tcttaccgac cggaatacag cttctttttc
    18661 caccttgaag ctgtcggttt ttgctttttc ctgcctgtaa acaaattcaa accggtaaaa
    18721 aagttctttc atcctttcag cattcatggc tgctttcgat ccatagtcgt tggccagcag
    18781 cataagcacc tccgatccac aaaatcgtcc gaaagcggaa gcaaactgcg ctttctccag
    18841 ttcctccggc agaaagccaa tcgggagcat cactttacgt aaatcaaaaa ccggaaattc
    18901 ttcctgaaaa aaaacatagg gaattcgtaa ctcccggcac atatccagct gcttctgaat
    18961 gtttttcctg ctgttatcca tcaattgaat gatcagaaac gacgtgtcat tgttttcaca
    19021 ttcgtcggct agtagcggtt tagtttctac cttcacaaat agctgaacat ccacaaaatt
    19081 attctccgac agatagttcc ggatgttatt ttgtacagat tctgcctgtt gcagattatc
    19141 cgcataacaa attattccga aatccttttt cagatcagct gccagccgtg ccgcactttt
    19201 cagcaaatta tcagcagtat ggatattatg aattaatgca gtcagcataa aaacaaccta
    19261 aatctccgta tggtaaccag tatataacct gaatttattg gattcttttt gttttctgat
    19321 tgaagtataa tcgtttgcta tcaactgata aagtaaacac gttttacacg cctcgataca
    19381 cttgtgagta tttcgtatgg gatagtctga agcttttccg ccacttcatc aatagagatg
    19441 ttttttccga agatttcaac ttcatcgcct tcactggcag gtatgccggt gagatcaatc
    19501 atcacagcat ccatgcacac atttcccacc acttttgcgc ggcatccgtt gacccaaacc
    19561 tctcctgttc cgttgcccaa atgcctgtcg aaaccgtcgg catatccgat cgggattaca
    19621 ccaatgcgac tgtcgcgggt gattttcccc ttccggctgt agcccaccgt atcgcctgct
    19681 ttcacatctc gtatctgcag aatgattgtt ttcagcgtac aaacagtagc taattttaca
    19741 tttggcaaag ccgttatgcc ataatgtcca atacctagac gcaccatatc gaactgaaat
    19801 tcgggaaaac gttcaatacc tgctgagttg aggatatgac gcatgatttt atggctgaaa
    19861 ggtttcgaca attcatccgc gcaccgggtg aaaagttcga gctgctgacg cgtaaaatca
    19921 tcgaactgtg gactgtccga tccaacgagg tgcgaaaaga cagaacgaac ctttagctga
    19981 ttctgattag cgagcaaagt tgtaagttca gacatgtctt ccggattaaa tccaagccgg
    20041 tgcatacctg tatcaatttt aatatgaatg ggataatcgg taagcgccag tttttcggca
    20101 gcaaccataa agagtttaag caagcggaaa ctgtaaattt ccggttcaag cttattgtca
    20161 aagattgttc cgaacgctcc cttttcggga ttcataacaa cgatgggtac ccggatacct
    20221 tcgcggcgta actcagcacc ttcatcgccc acagctactg ccagataatc gcagcggtta
    20281 ttttgaagtg tacgtgccac ttccaccgaa ccgctgccgt aagcaaaagc ttttaccatg
    20341 cacatgattt tggtttcggg acgcagtttc gaccggaaat agttcagatt gtccaccagt
    20401 gcattcagat caacatccag tgttgtttca tgtacaatca gttccagttt ggccagaata
    20461 tcctcgaaat gaaaacttct cgatcctttg agcagaataa tttctttgct gaattcgcgc
    20521 atcagcggcg atttgagcag atcatcggtg gtacggaaaa agtatttttc ttcggtctga
    20581 aatctatcgg cataactcga aataacctct cccactccta tcatccggtg aatattcttt
    20641 gattgtacca gtctggcaac aagttcataa agttcttcgg atgaatgtcc gctctgcaat
    20701 atatccgaaa gtatcagggt acgcgacaaa ttctttttgg ttgactgctg gttgagaaaa
    20761 ctcagggcta tgctcagcga gttgagatcc gagttatagg tatcattgat aagcagacaa
    20821 tcgtgatttc cctctttcac ttcaagcctc atggcgacag tttcgaggtt gagcagcctt
    20881 ttattaattt ctacagactt ataattcaga taaagcatcg tgcaaatgca ttgcatggca
    20941 ttttctatgg atgcatcatc ggagaaatta atattcagtt cgaacttctt ttcctgatat
    21001 tttaaataga gtattgttcc ggtttccgtt ttctccgttt tcagtacctg aacggtggta
    21061 tcggctttct tgccccatga aaaaagacgc gctttcaatc ccgcttgtgc aattgaaata
    21121 tctaccagct tattatctga attatagatt agcacctccg aatcaatgaa aagcttcatt
    21181 ttttcatcac acttctgttt cagacctgtg aaaccttcct gatgcgcgtc gcccagattg
    21241 gtaaatatcc ctattgtagg acggatgatg ggctgcagtt tctgcatttc ccctgtccgg
    21301 gatatgcctg cttcaaaaat tgccaactcc gagctatcac tcaacgacca aaccgaaagc
    21361 ggaactccta tctgtgaatt gtagctgcgt ggtgagcgga caatcctgaa atcggtgtgc
    21421 agcatctgat aaagccattc cttcaccacc gttttaccgt tgctgccggt aatacctatc
    21481 accggaatgc tgaattcagc acgatgggca gccgccagtg tttgtaaagc ctgcagcgta
    21541 tcatccatat ggataaatac ggcatctgtc agggtttcga aatcgggaag catttcgcta
    21601 accacaaaat accgtaaatt ttggcggtag agttcagcaa tgtagcggtg accatcgttg
    21661 cggcttgttt ttaaggcaaa gaaaagtgtt cccgacggga aagagagcga acggctatcg
    21721 gtgagcaacc agtgaatatc gacttcagga atgttattgt catcagtacc cagtaaatga
    21781 gctattttgg aaagtttcat agatgtaatt attgagatgc aaagttaaag aaaaataccg
    21841 acagaaagga aaaacatttt cctttattgt ccgggatgat agagtttcgt ctctgtcaat
    21901 ctttcttcat attattcgac agaatcggag ttctgtcgtc cctatccgat gttttgtatc
    21961 tgtttcctgt ctgtaaaaat cggaaggcta ataatttaca gcttaatttg ttctgcagtt
    22021 tgaggcgcag ctttttcttt attttttata ttttcttccc gatatagaac acgtatccgt
    22081 aatattcctt gtacttatca tacaaatgag cttcatgtct ctcattggca accagttctt
    22141 cggcagcagt atttcccgga tatttttcaa gaaaaacttc ctgtgccttt ttctgaggta
    22201 catagaaatt ttctgtccag catttttcag gcaggataaa ttcagccaca ggaatatatc
    22261 cggcattctg tagttgtaac attttcgctg ggatggtatc aatttcggga taggcatcat
    22321 tccagaaatc gtcaatttca gtgggacgat aatcggtaaa ccacgaagcc tccgttacgg
    22381 caatatatcc tcccggtttc agaaacttac gccattcgtt caccccgcgt tcaaagccaa
    22441 tgttgtaaat ggctccttcc gaccagatca tatccagttc tcccggctga aaatcgagct
    22501 gatccattga acccactttg cctgtcaccc gctcctgcag gttcagcttt gccgcgttcg
    22561 cgttgaacag atcaataaaa tccggaaaca agtcaatgcc ggttatcgtg ccgggcgcgt
    22621 gctgtgccag catcattgtt tgtccgcccg taccgcagcc aatatcagca attctggatt
    22681 cggcggtaag attatcgata aaactcagtg cacggagcgt tacttcgggg ctgccaggac
    22741 cctgacgttc gagagaggag aaatactcgc agataagctg catgtcgaac tcgtgaatgg
    22801 atttggtgtc gtcgttcatg tggataaaaa gtttgaatta agtgttaagt aacctgcaat
    22861 ccgcaaatgt atcatttttc tttcgttccg gttgtcatta ttgaatataa attgagaatt
    22921 gtgttgtccg ggaaatctcg ggatgacaga gttccgactc tgtcaatctt tcttcttttc
    22981 ttttttcgac agaataggag ttcttttgac ccggatagtc atgaatgatt gagttccgac
    23041 ttttctgctg cccttttata aaaaacagaa aaggcctgcc ctctcaggca agtcttttct
    23101 gtctttaata aaccaaattc acattatcat atgggcaaat tatagctcgg ttcggttgta
    23161 atttcgggaa caagttcggt gtacgttacc actccgtttt cgtctaccac taccacagcg
    23221 cgcgcgcaaa gtccttgcag cggaccatct gtctgcaata ctccgtaatc ggttgcaaac
    23281 gtattgtagc ggaaatcgga tgctgttacc actttgtcga ggccttcggc accgcagaaa
    23341 cgcgacgcgg cgaaaggcag gtctttcgac acacatagta caaccgtatt ttctttttcc
    23401 gaaacccatt tattaaactg acgcaccgac atggcgcata ctccggtatc gatactcggg
    23461 aagatattca acactacttt ttttcctgcg aaatctttta aggttacttc gctcaaatca
    23521 cttttaacca gtttgaaatc gggagctttc gtgcctacag caggaagttc accgttggta
    23581 ttcacagggt tacctttgaa atttgttgtt gccattttct atattcaatt ttttagtaaa
    23641 aataagattt tgttaatatg caatatataa caaggtcagg cgaattttgt ttatctttgc
    23701 aatgaagaat ttaacttaaa attcccaaat aaacatcgca tatataattg cagtaatttt
    23761 tcgggcaatt aaattccatt gtagtgaaca aaaacctcac ctatattctt ttctttgcag
    23821 ccattccggc tctcttttcc tgcaaggaca aaaccgaata ccgtgtcgga agtgattttg
    23881 aaatttatgt acagcgtttt gaacaggaag ctgcaacccg taaccggaca tttaacttca
    23941 aaagcagcgg actgattatt gaattttcca atctgaagga aggtgttgcc ggactgtgca
    24001 actatgaaaa acccatccgt attgaagtgg ataaagccta ctgggactcg ctgggattac
    24061 agaatggcac tgatcagatg cgcgaagagc tgattttcca cgaaatggga cacggaatac
    24121 tgaacagaac ccacaccaat tccgttttaa taaatgatga atggaagtcg atgatgtgtg
    24181 gcggcaacgc gatatccgga cgaacctgga acttgaatta tcatggtgaa cgcagagcat
    24241 attaccttga tgaactcttt aacgaaagcg cacccatacc ggcatttgcg gcagaaagtt
    24301 tcaacgccga cacaacaaac tttatagagg tttaccgcga cgaatttacc agtacctact
    24361 caacaaaatg gaaactggga caaacaaaca atggcaaagc aaccatcgaa aacagcatgc
    24421 tgaaatacga gtcacaaagt tcagtcaatc tgattgtcct gatagcgaca cctgttaatg
    24481 tgctgtccga tttcagcttt gaatgtaccc ttcagtatac cggtgccgat atctcgaaat
    24541 atggtattgt gtacggaacg tatgccgacg aaaaccaaca gactgatgca tcaggtgcat
    24601 cgctggaata tcttctgata aacaacgatc ggaaagcaac catcggcaac agatcctggt
    24661 tcagctattt tactcaggta acacgcgacc agatagtgcc gaaaagcctc aacaaaataa
    24721 aagtggtaaa gaaatgcagt atgatgtact ttttcatcaa cggacagtat gcctaccgtt
    24781 cggagatggt taacagtgcc gccggatata acttcggatt ttcggtgccg ccaaaaagta
    24841 cgctgtatgt cgataacttc agaatagctc agagtagtgg cagcatgaat gcgaaattca
    24901 gaatgaaaaa ggctgaaacc tctcaatttg agatcaggga gataaaaact gaactcggca
    24961 ccagaacaat taaagacaag taacaataaa cggaagcttc aaaaaaacag tataagcatg
    25021 gcaaaatttg taatttatca ggtactcccc cgactattcg gcaattacaa ccccacccgg
    25081 aaacacaatg gaaccatcta cgaaaacggg tgcggaaagt tcaacaactt caccccgaaa
    25141 gcatttgaag ccatcaagga actgggagtt acccacatct ggtacacagg aattattgaa
    25201 catgccacca aaaccgatta cagctcattc ggtattcgca gggatcatcc aggcgtggtg
    25261 aaaggaaatg caggttcgcc atatgccatc aaggactatt acgatgttga ccccgacctg
    25321 gccgatgatg ttcccaaccg gatggcagaa tttgaagatc tgatccgacg gacacatgac
    25381 gccggattga aagccattat cgatttcgtt cccaaccaca cagcccgtct gtattattcc
    25441 gatgttaaac ccgaaggcgt acaggacctt ggagaaaccg ataacccgaa actggcattt
    25501 tcgccgcaga acaactatta ttatactccg gaaactgcct ttaccccctc cttcgacacc
    25561 ggcggttata ccgaaatgcc ggcaaaagct accggaaacg accagtttac tgccttccct
    25621 tcggaatacg actggtatga aaccgtaaag ctcaactacg gcgtcaacta tgtggaaggt
    25681 atgcagaaac atttcgatcc gatacccgac acctggctca aaatggtgga cattctgtgc
    25741 tactgggcag ccaaagaagt ggacggtttc cgggtggata tggcagaaat ggtacctgtg
    25801 gaattctggg gctgggcaat tccgtttgta aaagccataa acccggatat tatctttatc
    25861 gcggaagtat atcagcccgc cgagtaccgc aactatatct gcaacggcaa gttcgattac
    25921 ctctacgaca aagtagggtt gtacgaaaaa ctacgtggca ttatcagccg taatgaacct
    25981 gcccgtgaca tcaccgctca gtggcaaaac ctgagcggca tcgaagaccg tatgctcaat
    26041 tttctcgaaa accacgatga acagcgcatc gcgtccggat ttttctgcgg cagcggagtc
    26101 tatgcccaac cggcaatgat tgtttccgca acactcacac aatcacccgt gatgatttat
    26161 atgggtcagg aactcggcga actgggcatg gacaaggaag gatttagcgg tgtggacggg
    26221 aaaactacca tattcgacta ctggggcgta tcgtccatcc aggcatgggc aaacggtgga
    26281 aaatttgacg gagtgctgct taccgaagag cagaaagaac tgcgtgcatt ttatcagaaa
    26341 ctcctgcaag tgagcatgaa cgaaaaagca atcacagagg gtgtaatgta cgatctggag
    26401 tttgccaact ttggtaatcc gaattttaat acccatgagc aatttgccta tttcagaaaa
    26461 cataaaaaag aattactttt gtgcgtcgta aattttcagg ataaaaactt cggaatggag
    26521 ataaatattc cctctgaagc atttcagttc ctgaatctgg ttgaagacga attttacagt
    26581 tcggagaatt tgctcaacag cgatgaagat tttccggttc agctgctctc atcgcaaagt
    26641 ccttaccggg tatttatgcc tgcctggtgc ggaaaaatac tgaaactgaa gaaaaagaaa
    26701 atctcgtgaa gacaaaaaga caaaagagca aaaacgcagg acattaagac aaaagataag
    26761 agtagaaaga taagagacaa aataatatca taacattaaa aaaaaaatta tgagtaaaat
    26821 tgaaaaaatc gtagcccgcg aggtacttga ctcccgcgga aatcctacag tagaagtaga
    26881 tgttacactc gaatcgggtg ttgtaggccg tgcttccgtt ccatcgggtg catcaaccgg
    26941 cgaaaacgaa gctatcgaac ttcgcgacgg tgacaaaaaa cgttacctgg gtaaaggcgt
    27001 acttaaagcc gtagaaaatg taaataaagt gattgctcct gccctcatcg gcgaatgcgc
    27061 actgcaacaa cgcaaaatag acaaagcgat gatcgctttg gacggaacaa aaacaaaatc
    27121 gaacctcggt gccaacgcca ttctcggtgt atcactggct gtcgcaaaag ctgctgccaa
    27181 ctacctcgac atgccgctgt atcgctacat tggtggaaca aacacattca cactgcctgt
    27241 tccgatgatg aacatcatca acggtggttc acactccgac gctcctatcg ctttccagga
    27301 attcatgatc cgtccggtag gtgcttgctg cttccgcgaa ggtctgcgca tgggtgctga
    27361 agttttccac tcattgaaaa aagtattgca tgacaaaaac ctgagcactg ctgtaggtga
    27421 cgaaggtggt tttgctccta atctggccgg tggtactgag gaagctctcg aatcaatcct
    27481 cactgctatc cgcaatgcag gttacgaacc cggaaaagat gtaatgatcg gtctggactg
    27541 cgcttcctca gaattcttca aagacggtat ctacgactat accaaattcg aaggcgctaa
    27601 cgctaaaaaa cgtacttcag ctgaacaggt ggattacctg gctgaactcg ttgccaacta
    27661 ccccatcgac tcaatcgaag acggtatggc tgaaggcgac tgggacggat ggaaactact
    27721 caccgacaaa ctgggcaaga aaatccagct tgtaggcgac gacttgtttg taaccaacgt
    27781 agaatacctg aaaaaaggta tcgacatggg ttgcgccaac tctatcctta tcaaggttaa
    27841 ccagattggt tcactcaccg aaacactcga cgctatcgaa atggcacacc gtgctggtta
    27901 cacatcagta acttcacacc gttcgggtga aaccgaagat gctactatcg ccgatatcgc
    27961 ggtagctacc aactccggac aaatcaaaac aggttcgctt agccgctccg accgtatggc
    28021 caaatacaac cagcttctcc gtatcgaaga agaactgggc gatctggctg cttacggatg
    28081 gaagaaataa taagagccta tccctaatcc ttctcttcac gcgagggact tcaaaaccgg
    28141 ttttcttttc agagaaccgg tttttttgtt tgtaaaatcc tgaaatataa ccgaaatatt
    28201 cataatcaga cgcctaagca tttttaatca aataccggca accgatatcg gtgaggttgg
    28261 ttccgcttct ccggttgtta tccttacatt tcagaccgga gttgggtctg attgtacggt
    28321 acattcctgt tggtaatcat aaaagtttta agtgatgata taatcctttc tcattaaagc
    28381 atttaattgc ctattacgat tcaggctaac acgattccat gcgaaaacct gataatagat
    28441 tgatgtcttc atactgcctc ccgattaatg attgaataaa aaattaaatg attttgtaaa
    28501 ataatagtat atttgcaagc taaaaaaaaa attaaataag tattcaatga ttaccataca
    28561 ggaagccaaa acaaaaaaag agatgaagaa attcatcatg ttcccctttg aattatacaa
    28621 gaatgacaaa acgtgggtac ctcctttgat ttttgatgaa tggaacacat ttaatccaaa
    28681 aaaaaatcca tcctttgaat tttgtgaagc tgcttttttt cttgcgctta aggaaaataa
    28741 agtggtagga cgaattgccg ctatcatcaa ccacaaagcc aacgaagcct ggaacgaaaa
    28801 acgcacccga ttcgggtgga tagcttttat cgacgatccg gaagtttcaa aagcattgct
    28861 tgataccgcc gaaaagtggg gcgccgaaaa aggaatgcaa ggcattcacg gacctcttgg
    28921 gtttaccgat ctcgatccgg aaggtatgca ggtggaaggt tttgaaaaca atgcaaccat
    28981 caccagcgcc cataattatc cctattttcc tgtacacatg gagcaacacg gatttcgcaa
    29041 atcggtagac tgggttcaat acaaattcaa cgcttcacag ccaattcccg aaaaagtcgg
    29101 acgtatcaac aaacttattg cccaaaagta taaggtcaac accgtagtgc caaaaaccaa
    29161 aaaggatgct ctgaaatacg ttccgggttt tttcaaagga gtgaatgagg gtttcaaaaa
    29221 tctatacgga tatagtcagt tatctgacaa acagattaaa tactacaccg atgcctactt
    29281 tggatttgcc aaacccgaat tactctgctt ccttaccgac gaaaacgatg aagtggtcgg
    29341 ttttggaatt tgcttcccgt cgctttccaa agccttccag aaagcaaaag gacgattgtt
    29401 cccctttggt tttatccaca ttctgaaagc gctgaggaat tacgatgaaa ttgacctcta
    29461 cttcaacggc gtggtgcccg aatggcaaca caaaggcatt cattcgctct actatgtggc
    29521 gatgaacgaa gtcgctattg aacgtggagt taaatgggca tattctaccg gtcagctcga
    29581 aacgaatgtc aatgctgttg gtatctggga taattacgat aaacaaccgc tgttccgtac
    29641 tcgctgctac ataaaagact aaaattcaga attataaaaa agatgtccgg taaaaaatta
    29701 ccggacatct tttttatact aactgtcttt tatctaataa aatccctgtt tacttcaatt
    29761 gtatttttgc tcttttaatt tccaggttag cttgtttttt ctcaatactt atctgccggg
    29821 cttttagttg taaagcttct tcataagttt tcaactcagt ctgtcgtgca tctacctccc
    29881 gggctctatc ttcaagtttt ttctgttcat tctctatctg agcctggctt ttacggagat
    29941 tatccatttt attagcccag tttgcattgg catcggctac acgcttttct ctttcgtcaa
    30001 atttcgtatt ccggaaatct acttcgttgc cgcttaaccc tttcgatgtt tttataaaat
    30061 cttttcgttc ttttaaaagc attttcaatt ccttttgacg tagttttttt tcagtcttca
    30121 ataatttctc gtcttcctta aagaatttca ctttctgttt gaggttactt ctttctttct
    30181 taactaactt cgcttccgaa tcggcctgat cctgatattt actgatttgt ttacgattgt
    30241 ttttaagtac atcttgaagc gaatctattt gcagtttata gccttcaaaa tctttttttt
    30301 gctgacgaat ggaatcgatg ttcactggtt ctaggatggg aatttcccaa tggtaaaagg
    30361 ttctgttaat gggttcaacc tgcgttgtgg tcttgagtgt tgctgtggtg gtatcatttt
    30421 tctgtggttc ttgtgcctgt acaaatcccg tacacaataa tgtaaacaat acagcaaaaa
    30481 tcaatttgta aattttcaat ctcataattc attttttttg gttaataact gcaaatatac
    30541 atatctttgg ctattgcaaa aaaaatgaat acattttttt gcaatatcat gccttataat
    30601 ataaaattac ataaaataag aatttgaaac tgcattcact ccagtattcg caaaatattt
    30661 tttccataca atttctgatt ataaagtaac attttatatt attttacccc ataattatta
    30721 cattccgtca aacaattcat gtttttttca tatattacaa cttttttgat gtcttattac
    30781 ccaaattgta agtatttttg caatctgaaa ccaagtaaac agaatgaaca tatgaaattc
    30841 acaaaaatgc acggcactgg caacgattac atctacgtaa acggctttga aaacatcatt
    30901 gaaaatccgg gcgaaattgc catccgcctg agcgaccgtc acaaaggtat cggttccgac
    30961 ggtctcgttc tgatacttcc gtccgaaacc tgcgatttcc gcatgcgtat gttcaattcc
    31021 gacggttcgg aagctgaaat gtgtggcaac gcctcccgct gtgtcggcaa atatgtatat
    31081 gacaaagggc tgaccaccaa aaccgaagta actctggaaa ctcttgccgg tgtgaaagtg
    31141 cttaaactct ttgtgaacaa tgataacaag gtggaaaaag tgactgtgga tatgggcgaa
    31201 ccaatcctcg actcgaaact catccccacc acctttgacg aatcaccggt gatcaacaaa
    31261 gtggtttcat tcggcaaata taccgattat caggttacct gtgtttcgat gggtaatccg
    31321 cacgccatca ttttcacaga aaatgtaagc gaactcgatc tgccgaaaat cggacccatt
    31381 gttgaacatg ccacatactt tccaaaacga acaaacactg aatttgttga agtaatttct
    31441 gaaagcgtgc taaaaatgcg cgtgtgggaa cgaggagcag gagaaaccat ggcatgcggc
    31501 accggagcct gcgcaacagc tgtagcagcg gtgctcaatg gagtggccga tcgaaaaacg
    31561 actatcgaac tgcttggcgg caatcttgaa attgaatgga gagaagctga taatcacgta
    31621 tatctgaccg gtgacgcggt taccgttttc gaaggcgaag tggaattata aaatatgaac
    31681 tataaactaa aaactattaa ctatccaatg gcttatatta acgacaattt tattaaaata
    31741 cccgcaacat atctattctc cgaaatagca aaacgcgttg cacagcacaa aactgaaaat
    31801 ccggaagttg acattatccg catgggaatt ggcgatgtta cgctcccact gcccgatgct
    31861 tcggtcgatg ccatgataac cgcagccaat gaacaacgca agagcgaaac tttcagaggt
    31921 tacggacctg aacagggtta tgattttctc cggaacagca ttgttgaaaa tgattttaaa
    31981 tcacgtggaa ttgatatcga agctgacgaa atctttgtaa gtgacggagc aaaaagcgac
    32041 accggcaaca tcggcgatat tttcggatta cagaaccgcg tagccattac cgaccccagt
    32101 tatcccgtgt atgtggatac aaacgccatg ggaggacggg caggagaacc ttccgccagc
    32161 ggacaatgga ccaatctggt ttatttgcct tgcaatacag ccaataattt tgtaccggaa
    32221 cttcccaacg aaaaagtgga cattatctac ctgtgctatc ccaacaatcc aacaggaacc
    32281 acccttacca aagagcaact gaccgtttgg gtaaattacg cccgtgagaa taacgcgatt
    32341 atcctgtttg atgctgctta cgaagcttat attactgaag aaaacgtacc tcacagtatt
    32401 tttgaaatcg agggagccaa agatgtagcc atcgaattcc gcagcttctc caaaactgcc
    32461 ggatttaccg gaacacgctg cggttatacc gttgtaccta aatctgtgac tgcattatct
    32521 gaaaatggag aaaaagtaag cctgaataaa ctctggcttc gtcgtcaaac cactaaattc
    32581 aacggtacgt catacgttgt gcagcgtgct gctgaagcaa cttacagctc ggaaggaaaa
    32641 aagcaggtga gagctctggt gaattactac atggaaaacg cccgcatcat ccgcgaatcg
    32701 ctgaaagcag ccggactgac tgtttacgga ggagtaaacg ctccctatat atggacaaaa
    32761 acaccggatg gcattagcag ttgggaatac ttcgactatc tgttgaaaga aaagaacatc
    32821 gtatgcacac ccggtgccgg cttcggaccc gcaggtgaag gatttgtacg cttttccgcc
    32881 ttcggaagtc gtgaaaacac cctgaaagcc atgaaaaggc tcgaaaaata aagtaacata
    32941 cctttcatat tcataaatcc cggtttgctt cctgcagatc gggatttttg tattcaaacg
    33001 ctatctttac catatcacct ggtttcggga ttgtcaaata ccggaggaaa cttttcaaaa
    33061 aaaaaaatcc ggttaaccat tcctgataac cggaaaacaa taacgatttt tcccaataca
    33121 tcctatcaat actcattttt cacaagagaa cagacttaaa aatcaaagaa atatttagca
    33181 ttaatcttac actacattac atttatcgca ttttttatat catattgaaa tttatttatc
    33241 ttatatagtt tccttttaac accaagcgaa gtttatttat atattattga tagcatttcg
    33301 ctgcatattg tgtctttttg aatacttttg cttcatatca caatcatttt gaatttcatg
    33361 ttcacatttt tcagtcatta tgacatttca ctaatcaaca acacatcaaa atgcaaataa
    33421 taatcaacaa actgactata tataaacaca aaaaaaatga atttatgaaa aaggtaaaaa
    33481 ttttattgat tgtcatgtta acaagcgccg cctttttaag catcgaggca caaggcgtca
    33541 gtattaatgc ggatcttgtc agtacgtacg tctggcgggg cacaaaatta tcaggtgcat
    33601 ccattcagcc actccttcaa tttacaaaag gcggattttg cataggctcg tggggctctg
    33661 ccggcttcga cggctatctg gaaatggact tattcacccg ttacacattc aacttcggac
    33721 tatctgccgg cctgaccgat tattactttc cgggaaccaa ctactttgat tacagcaaag
    33781 acaccggatc tcacggatac gaagttaacc ttggctacgc tgttcatgga ttttctatcg
    33841 gagcaaacta catgctgaac gaagcaggag gtgccaagac tgccggcggc gacaaatact
    33901 tcgagctggg ttattctctg aagaacataa gcatatttgc cggtgcaggt gatggatggc
    33961 acacccctac gggcgatttc ggcattgtaa atctcggatt gagcgcaaca aaagaattat
    34021 cggtatcaga aacactccgc attcccgtaa aactaagcgc tattctcaat cccacaacaa
    34081 aacaatatta tctcactgca ggcattacac tgtaatctgt caattcaaaa agacagtaaa
    34141 agtacaattt atcattttga aatgttaact aaataatcac tgtacatttt gatactcata
    34201 aaaggcattt tgaagtatta ttttgttaaa tgtgattttt atttatcctt ttgtttggca
    34261 atataaatta tttatgtact tttgctgccg aaaacacttt atgaattaca gaatgtaata
    34321 caattcaatt aaaaaaacga ctaataagca ctgaaaatga agaaaattga agcggtaatt
    34381 cgcaaatcca gattcgaaga tgtaaaggaa gcattaaacg aagcaggcat cgagtggttt
    34441 tcatactggg atatcaccgg acttggcaaa tccaccgaag accaggtagt aagaggacag
    34501 attttcaaat caaattatat tcagcgtcgc atgctttcta ttgtagtacg cgatgtgaac
    34561 ctcgaaaaaa ccgtcaacgc tattctgaaa tcagcccgaa caggcgaaat gggtgacgga
    34621 aaaattttcg tctatgacat tgaggaaact taccggatca gaacaggaga atccggaccg
    34681 ggcgcacttt ataataaaga aaattctgta aatctttaat ccctcaaaaa aaaacaaaaa
    34741 tgaaaaaata tataataact cttttggcat ttttttcggt atcgattaca actatgtcat
    34801 cacaaacttt agtcgcgaca actccgacta tcaactccgg tgataccgcc tggatgatag
    34861 tggcaaccgc attggttcta tttatgtcaa ttccggctct tgctcttttc tacggaggtt
    34921 tggttcgccg taaaaacgta ttgaatgtat tcatgcaatg ccttatcatt gtggctgtca
    34981 tcagcataga atgggtagtt tgcggctaca gcctctcttt cggaagctct cacggatttc
    35041 tgaccccctt tatcggtggc ttcgattggg cttttttaaa caacatccat atctccgatt
    35101 taagtccgta ttttatttca cattcacaag ttgcagctga cggcaccagc accgggacta
    35161 ttcctcacct tgtatttgtg atgtaccaat gtatgtttgc tgtcattacg cctgctctga
    35221 ttatcggggc atttgctgag cgcatcaact tcaaggggtt tttgatattt accattttat
    35281 ggtccttact tatctacaat ccggttgctc attgggtttg gtcagctgat ggatggctgt
    35341 ttaaacttgg agctcttgat ttcgcaggcg gcacagtagt acatgtcaat gccggaattg
    35401 ctgccatcat cacagctata atgctcggca agcgccgcga ctacgatggt cacgcacttc
    35461 cacctcacaa cattacctac gtggtgatgg gtgcagccat gctttgggtg ggctggtttg
    35521 gctttaacgc cggaagcgga cttgcagctg atggactggc cgctaacgcg ttcatggtca
    35581 cccacattgc tgcagcagcg gcagcactca cctgggctat tctggattgg ttcaacgaca
    35641 agaaacctac tatcgttggc actagcaccg gcgcagtggc cgggctggtc gctattactc
    35701 cggctgccgg ttttgttggc gtaggcggcg ctttggcaat aggagtggtt gtatcactga
    35761 tttgtttcgt catggtcgcc cgagtaaaac ccaaattggg ctacgacgac tcgcttgacg
    35821 ctttcggagt acatggaatt ggtggtatga ttggcgcaat catgacaggc ttgttggcaa
    35881 ccccattaat tcaaccggct tacagtggag ccttttacgg caactggcat cagttggtgg
    35941 tccaactgat agccgttggc gctaccatca tatattcagg cataggcact tttctcatat
    36001 ttaaggttac agaatcaatc attgcgatca gggctaacga taagcacgaa gcaataggat
    36061 tagatgaaac tcaacacgga gaaaccgctt acacaagctt tgaataaaaa taaaaaaaga
    36121 caaacagaca tagagatgtc tgtaaggata tattttgaaa gaaattttag tttagtatgg
    36181 tttacttcta ctcgtatgag cacctgtaaa ctttagataa gatacagtaa aactgatata
    36241 ttttggtatt actgtaagtt ttgagtttta ttgtttcagg cagactactt cgcgatgaaa
    36301 tagtctgcct tatttagaaa atcattttgt aatctaaaaa cacactttca ctgatatttc
    36361 attcattatt ttcacgatta attatatttt tgaaatcatt tctgcagaat tcaacaataa
    36421 acaatcatca ttacacaaaa cttcatgtgt ctattcgaat tatttacagg tcttttaatt
    36481 tcattttata acaattgatc tgtttttttc aacacataat aactttatag ataatattat
    36541 taaaataaca ataacagaga gaaaatccct atcaaaaatg tctatttttg cataaaaata
    36601 acaagctaaa ttacacatca aatagaatct caaacaaaca aaacaataat acaaagatta
    36661 tgtcatcctt aagatttaca gcagtagaag aagcattcaa gaaaaaagca ttatgcgttt
    36721 cagctccttc aaaat
//
LOCUS       DAOPYB010000013        36106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1064_length_36106_cov_9.465868, whole genome shotgun sequence.
ACCESSION   DAOPYB010000013 DAOPYB010000000
VERSION     DAOPYB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 36106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36106
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1064_length_36106_cov_9.465868"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(409..>1698)
                     /gene="feoB"
                     /locus_tag="PKH58_02025"
     CDS             complement(409..>1698)
                     /gene="feoB"
                     /locus_tag="PKH58_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNX87834.1"
                     /translation="WGFPIFLFLMWLMFQATFTLGSYPMEWIDAGVSLLHDWLQTSMA
                     EGPLHDLLVDGIIGGVGGVIVFLPNILILFFFISLMEDTGYMARASFIMDKLMHKIGL
                     HGKSFIPLVMGFGCNVPAIMATRTLDNKKDRILTMIITPFMSCSARLPVYVLLISAIF
                     PTNQGLVLFSIYVIGIVLAVLTALVMKRVAFAKKEVPFVMELPPYRIPTLKNTTIHMW
                     HKGQQYLRKMGTVILVASILIWALGYFPRNVEYSKDYDTQIASVQTNPQLSELEKENQ
                     VSHLTVEQEAERQEKSYIGQLGHAIEPVISPLGYDWKMGVSIITGLAAKEIVVSTMGI
                     LYQADLQADENSGTLKANLHKQTHLTGKLKGQKVFTPLVTYGFMLFILIYFPCVAVIA
                     AIKKEANWGWAIFTMVYTTVIAWIVAFATYQLGSLFV"
     assembly_gap    1699..1798
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1799..2754)
                     /locus_tag="PKH58_02030"
     CDS             complement(<1799..2754)
                     /locus_tag="PKH58_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNX87835.1"
                     /translation="MTTDITYLSDLQSGDEAIITKVLGHGAFRKRITEMGFVKGEKVR
                     VIKNAPLQDPVEYEIMGYNVSLRRSEARLVEVVSVEEAAGLAEVKFEGTIDEETLKVS
                     ALKKGKTIRVALVGNPNCGKTTLFNYASGSHERVGNYSGVTVDAKEAVMKKDDYTFKI
                     YDLPGTYSITEYSPEELYVRTHITESNPDIVVNVVDASNLKRNLFLTTQLIDMNIKVV
                     IALNMYDELEKKNVKFDYDSLGQMIGIPIIPTVASKGKGIDELFSKLIDVYEDRDESV
                     RHIHINYGQLIEEALKEIQDEIWKNPEITDKLSSRYVAVKLL"
     gene            complement(2776..3906)
                     /locus_tag="PKH58_02035"
     CDS             complement(2776..3906)
                     /locus_tag="PKH58_02035"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNX87836.1"
                     /translation="MRLINKSVFAAVLISTFSITGLSAQEADSVKKTSPFTFEASYIG
                     DLVSNFSGGIKTGTTYLGLANIKAGFDTEKANWWKGGEFFINVGNTHGGEPSANLVGD
                     FQGVSNIEAGNLTFMYELWYKQTLGKFDLTIGLQDLNANFAVSENGALFTNSSFGIHS
                     SIADNITSPIFPLTALGATLQFRISDTFNWQAAFFDGTPDDFERNPYNIHWKLGNDQG
                     LLAVTEFQINKSLIKGRDAVYKLGTYYHKHNNTSLNNFKNGGFYFVADQQITDKLSLF
                     SQLGLSSKKQNNNNYYGSIGMNYRELFKNRKNDLFGLAVNHAGINGNGIGSETAVEMT
                     YHYQLCKYIYLRPDIQYIINPAGTETKLKNALVGFLRFGVDF"
     gene            complement(4087..4695)
                     /locus_tag="PKH58_02040"
     CDS             complement(4087..4695)
                     /locus_tag="PKH58_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX87837.1"
                     /translation="MNGNPLKIAIVEPSVIVRSGLSAVLKRIPGYRIQPVEILTIDSM
                     NHYLNIHKPDVLIINPSFWGYFDIAKIKTETTNGRIICIALVYSVIDEHLLRDYDERI
                     SIYDTVDQIREKFEKLFSSPPVENSEEPNTLSTREKEIIACVVRGMTNKEIAQNLFLS
                     AHTVITHRRNIARKLEIHSTAGLTIYAIMNKLVELDDIKLNN"
     gene            complement(4692..5399)
                     /locus_tag="PKH58_02045"
     CDS             complement(4692..5399)
                     /locus_tag="PKH58_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNX87838.1"
                     /translation="MFKQHKFSETDKMSDLINENHSLLLVISRFGLSLGFGDHSVKQV
                     CSENGIDCRTFLAVVNFLTEANFEVDHDYEDISIGSLINYLKNAHVYFLEFKLPQIRN
                     KLIEAVSSHDQNVPYLTVFLKFFDEYVSEVRKHMEYEDKVAFPYVIRLLNDEPGLIYN
                     ISVFRHRHNEIESKLVELKNILVKYYPDKGNNPLLTEVLFDVFSCEMDLATHSQVEDF
                     LFIPAVEAVERKRNLKK"
     gene            complement(5732..6835)
                     /gene="meaB"
                     /locus_tag="PKH58_02050"
     CDS             complement(5732..6835)
                     /gene="meaB"
                     /locus_tag="PKH58_02050"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNX87839.1"
                     /translation="MHYRHHHPENDPNYKGLSVNEGVSDVPTINPYAKPRIKRQLLTT
                     TEYVEGILRGDISILGQAVTLVESSLSQHQEIAQEVIEKCLPYAGKSVRLGITGVPGA
                     GKSTFIEALGMHLIHDGKKLAVLAIDPSSERTKGSILGDKTRMEELSVAKNAFIRPSP
                     SAGSLGGVARKTRESIILCEAAGFDVVFVETVGVGQSETAVHSMVDFFLLIQLAGTGD
                     ELQGIKRGIMEMADGIAINKCDGTNIEKSQLARRQYLNALHLFPLPESGWKPDVVTCS
                     AMERSGIAEVWEMVLRYIGFVRDNGFFEIRRTDQAKYWMYETINDHLKSGFYNNDKIQ
                     ILLEEAERRVLTDEVSSFVAARQLLDAYKSLNR"
     gene            complement(6933..8021)
                     /locus_tag="PKH58_02055"
     CDS             complement(6933..8021)
                     /locus_tag="PKH58_02055"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNX87840.1"
                     /translation="MIKRIGFLVIISVLTTIAVMGQKPTITFEEKTFDFGTINESDGK
                     ATHVFEFTNSGSTALLVQRVNASCGCTTPEWTKTPIEPGKKGEITVTYNPAGRPGAFT
                     KNITVYSNASNEVESLIIKGTVNPKSTAAAENYPVQAGDLKLRTKMIQMSNVTKGSTR
                     TNTLLVKNGSNTKLTVAVSDLPVYLKAEAKPDVLNPNEEGKIEFVFDSKKCAEWGPVY
                     DNVLLVLNGKKVADDAHKITINATVMDDFSKMTIDEKRQAPILEIKSPNLILGSMKQG
                     SIVRGKAIIKNSGQKALEIRRVINNNSNITVHPGKLSIRGGKTGELKIDIDTKAFPVG
                     DYKKSFIIQTNDPGNSFMTYTISWSVKK"
     gene            8220..9401
                     /locus_tag="PKH58_02060"
     CDS             8220..9401
                     /locus_tag="PKH58_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNX87841.1"
                     /translation="MLKKFKNSALSVFDYTTACYRHLHTHPELSFQEVATSAFIQKQL
                     TDLDIPFRANVAGTGVLGRIEGKNPQKKLIALRADMDALPVQEAVDIPWKSLNSNIMH
                     ACGHDAHVACLLGAAKILKEHCSEFEGTILLVFQPGEEKAPGGASLMLQDGIFDEYKP
                     EFILGQHVSVDFPVGTMAFLPGTIMASADEIHLTIHGKGGHGALPHLCNDTVLAASQT
                     VVALQQVRSRLCHPLTPMVLTFGKLIADGATNIIPSEVKLSGTFRTVDETWRSEGLKH
                     IRRIITETCAASGCSVDIDIPSGYPCVINNSELTLKAGQFAAEWAGAEHISQLELRMT
                     SEDFGFFSQQFPSCFYRFGVKGTSNQNCGGLHSPTFCIDENALLTGAGGLAWIAFRLI
                     S"
     gene            complement(9398..9931)
                     /locus_tag="PKH58_02065"
     CDS             complement(9398..9931)
                     /locus_tag="PKH58_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX87842.1"
                     /translation="MEIIQIKSADDEHIHSLMPLYQEAFPENQRHTEEDFKALLGSEP
                     DFYCNAVLMNDNFVGFFNYWDFDWFIYAEHFAILEAIRGNKLGEKVMNMMKPQSDIPM
                     VFEVELPDEEMAARRIEFYRRLGYEVVPVDYQQPPYNRNGYYQPLFLMTDQVDFVIEN
                     FEKIKSTIYRRVYKVEL"
     gene            complement(10138..10707)
                     /locus_tag="PKH58_02070"
     CDS             complement(10138..10707)
                     /locus_tag="PKH58_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX87843.1"
                     /translation="MKIEINKVVDAEYELYVDGENGEPELMERATPEQPLNFIYGVGM
                     MLPKFEENLNGLQKDDNFEFTIKNEDAYGEYDDDAVVELERAVFEIDGKLDEEMVFEG
                     NIVPLMDNEGNRLQAQVVSVTDTHVTVDLNHPLAGETLHFKGKVLGVRDATEQELKSL
                     LGGGCSCGDGGCGCGDDCDDNHGCGCGCN"
     gene            complement(10764..13517)
                     /locus_tag="PKH58_02075"
     CDS             complement(10764..13517)
                     /locus_tag="PKH58_02075"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNX87844.1"
                     /translation="MLSSKQLYNTKLTVLFLLLACFTYATHASGQKSTPFDNSKNNLL
                     PTDSQKVASVNDSSKLTKPVNPNQIDAEIAYTASDSIVFLGDGTGFLYGKTNLSYKKI
                     NLKEADFVRISTDSSTIFARGVPDSLGVMKGKPVINDGDQEYNATELTYNLRTRKGFI
                     RQAVTQQGEGYIISDKTKKNDDDVLCMQDGMYTTCDNHEHPHFYLSLSKAKVKPGSYI
                     ITGPAHLVLLDIPLPVAVPFGFFPFNKNYSSGLEMPSFDTDVTRGYGLVNGGYYFAIS
                     DYADLSIKGDVYTRGTWGVNLSSSYLKRYKFNGSVNVSYREDVIGEKDMPDYQKSPNL
                     SIQWSHSQNPKVNPFRTFSASVNFTTSGWNRSNINSYYTPANAENTKSSSVSFSQRFP
                     NNPFSITANMSVTQRTKDSTINLMLPNVAISMSRIYPLKRKNPVGNERWYEKISMSYS
                     GTITNSIQTKENKLMHSSLAKDWVNSAQHNIPVSASFNVLKYITFTPSFNYKEKWFLT
                     SQRKTWDVVNQKEVTTNVTGFNRVWDYNLSMSANTTLYGMYTPVKAIFGDKIEKIRHM
                     LKPSASFAFTPDFGDLKYGYWDTYIKSVIKDGDPTKITSEEIYYSRFPSGAPGRGKSN
                     NLSFTLDNNLEMKVKNNEDTTGLKPTKIISLIDNFSLNSAYNFAADSMQWSNIGTSLR
                     IKFGKAYTLSLSGAFDPYMYVSPDGKSIKRVNQLRWEHGKFPRFLGTSTSYSYTLSNE
                     TFKRKDKKSKGNTNTESDEDTGNSDINADSMRSQQKDSNSGTDKTTTDADGYEKVEIP
                     WSLSFNYSVRFGNSSEFNYEKLEYKQVFTHNLGITGNIKLTPGWQISGTTSYDFNAKQ
                     MSYTSFNITRNLHCWTLSGNVVPFGPYKTYNFRIGVNSSMLQDLKYEKQGNRSSPNNI
                     TWY"
     gene            13597..14931
                     /locus_tag="PKH58_02080"
     CDS             13597..14931
                     /locus_tag="PKH58_02080"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNX87845.1"
                     /translation="MNNLIFYNLLNASTLDKITTFASNYNVFMNSSLFFRKTMKYLRY
                     TTFIIILILTCIVSPSYAQKKFTVVIDAGHGGHDSGAVGRYSKEKNINLAVALSLGSK
                     IEASYPDVKVVYTRDQDIFLTLQERADIANNNNADIFFCIHTNANKSSTAFGAETYTL
                     GLHKSQSNLDVAMRENSVITLEEDYKARYQGFDPSAPDTYIMFEFMQDKYIDKSIELA
                     SAIQNQFVQTRRYDRGVRQAGFWVLAKSACPSVLVELGFISNRAEEDYLNTSEGRDEM
                     ANSIFRAFELYKREHDKKSGIKVTPLKKVDLPTPYKEVEKPVKTETPVLAAVSTKTDT
                     TKELKIRPETDKTIQTVTKPDEAKKTNEVVFKIQLFALEKKLKTNAPDFKGLKNADFY
                     VENQLYKYTYGEESNFSEINKLRKSISKKFPNAYVIAFRNGEKISVKEALKK"
     gene            14966..15877
                     /locus_tag="PKH58_02085"
     CDS             14966..15877
                     /locus_tag="PKH58_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNX87846.1"
                     /translation="MKKNIFTREVKVGLMVIAAIFILYFGLNFLKGIDIFQKTNLYYG
                     QYEDIGGLVPSAPVYIKGYKAGQVEEVKYDFSKKESFLVKISITSDIKLPEGTIMQLA
                     DEGLMGGKVIQLIYAPVTAGQKMHKPHDILPTEVSTGLMATVAGDLMPKIENVATQAD
                     SLLRSVRTLIERKEISNSLSSIEKTTADLSASSAQLKGLLNNQLPSILNDVNTVTGDF
                     KQLSGNLRNIDFAKTVNSVDYTLKNLQTITDKINSNESSLGLLMNDKTLYINLSNTAN
                     AADKLLIDLKENPKRYVSFSLIGNNKK"
     gene            16294..17691
                     /gene="dnaA"
                     /locus_tag="PKH58_02090"
     CDS             16294..17691
                     /gene="dnaA"
                     /locus_tag="PKH58_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNX87847.1"
                     /translation="MSYHYQLWNSCLNIIKDNVNESSFNTWFAPIVPVKFENKIFVLQ
                     VPSQFFVEYIEEKYIDLLRMSLTRVAGNGTKLEYRVLIDKSSGTGTQIPSTAETSKPV
                     QTNYSSQQNAIVSPYQRLQLPEIDPQLNPSYNFSSLIEGRSNKLARTAGMSIANDPGK
                     TIFNPLFMYGKSGVGKTHLANAIGVMTKQQFPGKRVLYVSANTFLIQYTDAVRNNTQN
                     DFLNFYQTIDVLIMDDIQEFAGKTATQNTFFHIFNHLHQTGKQLVLTADRSPLEMAGL
                     EQRLLTRFKWGLSAEIEKPDYELRKSILESKIYRDGLEIPENVVDYIAENVVDNVRDL
                     EGVLISLLAHSTLTNMPIDIRLAEKIISRIVNITPKVTTIEQIREVVCDYFKLSVDAI
                     STKSRKLEVVQARQIAMYLSKNMTKNSLSMIGNYIGQRDHATVLHACKKVVDLMDTDK
                     HFRKSVEEIEERLKK"
     gene            17833..18393
                     /locus_tag="PKH58_02095"
     CDS             17833..18393
                     /locus_tag="PKH58_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87848.1"
                     /translation="MIFKFTIISDENDDFLRIIDIDSEASFLDLHNAILEAVDFEKNQ
                     MTSFFLCNDDWEKEQEITLVEMESSSEYDNLVMEDTFLDQILTDEKQKLLFVFDMISD
                     RAFFMELTEMIPGKDINEAVCVTSTGLPPVQIADDTDFITPQKPNLSSEESFYGDEDF
                     DIDELDEEGFGDLNFDESSLFTDDNH"
     gene            18462..19394
                     /gene="miaA"
                     /locus_tag="PKH58_02100"
     CDS             18462..19394
                     /gene="miaA"
                     /locus_tag="PKH58_02100"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNX87849.1"
                     /translation="MIMSTLIVITGPTGVGKTNISLQLAEKFGCPIISADSRQFYREL
                     KIGTAAPSEEELQRVTHYFIGTHSIFDEYNAGQYEEDVIVLLDELFQKHSVVLLVGGS
                     MMYVDAVCNGLDNIPTVDVETRRFWQKEYAAKGLEFIQNELRRLDEKHYNEVDLKNPK
                     RILHALEICTMTGNPYSNLRTGERKKRNFEILKIGLNLPRPELYERINRRVDQMMEAG
                     LLEEASQFYPHKHLNTLNTVGYKEIYAFMDGEWDIETAIGMIKQNSRRYAKRQLTWYN
                     RDAEINWFRPDDEKSIMDLITSQLQIKDNGPIIN"
     gene            19408..20418
                     /gene="murB"
                     /locus_tag="PKH58_02105"
     CDS             19408..20418
                     /gene="murB"
                     /locus_tag="PKH58_02105"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNX87850.1"
                     /translation="MEIRQNISLKPYNTFGIDVKAKYFAEYNSVTELKELISSEVFCS
                     NRHFHIGGGSNLLLLNDYDGLILHSQIKEIEKTGEDEEFVFLRVGSGMVWDDFVAYCV
                     SNNYYGVENLSLIPGEVGASAVQNIGAYGMEVKDAIFSVETIETATLQNRIFSREECR
                     YGYRKSIFKEELKGQYIITHVTFRLKKNPDYQLDYQHLKQAVLERGDINLNNIRETVI
                     SIRQSKLPDPAISGNAGSFFMNPVISAEKFEMLQQIYPTMPHYPVSQSEVKVPAGWLI
                     EQSGLKGKSFGRVGVHSKQALVIINLGNATGQEVADLAAMIMENVWQKFGIEIRPEVN
                     FI"
     gene            complement(20470..20952)
                     /locus_tag="PKH58_02110"
     CDS             complement(20470..20952)
                     /locus_tag="PKH58_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNX87851.1"
                     /translation="MKKSDNIEIERKFLVRGDFRPFVSRSFRITQGYLCASPERTVRI
                     RIKADKGFITIKGKTGNTGISRFEWEKEITKEDAENLLLLCQKGMIDKVRHEVEYDEK
                     IFEVDEFFGENEGLLLAELELQSEDESYTKPDWLGTEVTGDRRYYNSYLSIQPYKTWK
                     "
     gene            complement(20975..23848)
                     /locus_tag="PKH58_02115"
     CDS             complement(20975..23848)
                     /locus_tag="PKH58_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNX87852.1"
                     /translation="MKSFLHRVAKAYCDEYKQDIYHFTFVFPNRRAGLFFQKYLSELI
                     DKPVFSPGILTINECFFSASQRKVADRTRELFRLFSIYRQLSGSEETFDTFCYWGEIL
                     LADFNEVDKYQIDARQLFTNVKELKELDTVFDQFTPNQKLAIQEFWQHFLPSTEGKTK
                     EEFITTWRILLPVYEEFRRQLFAENTATEGMIFREIAEQLKRKEDPEFFAGKKFVFVG
                     FNALNPCERMLFAELQKRGQADFYWDYEASELRDDQNQASLFYRENVHFFTGPLKIEP
                     SNSELSCKSFELISIPSAVGQTKEVYRLLQNILPENNESPEWIKTAVVLPDENLLIPM
                     LHSMPPNVEKINVTMGFPLNATPVSGLLDNIFELQRRMNKHQMFYHRTVSNILNHQYI
                     HLHCSEKARGIDEMMIRTNSMYVGKDAFNGNDLLTAIFKPINTSDGFIDYLLEILKKL
                     NIAWEQKANEKNAYRLECDFLHQYYLALNRLKTVIAETAADTEISLDTIMRLIRQLTA
                     GISIPFEGEPLDGLQIMGMLETRGLDFDNLIICSFNEGVFPKKNTSNSFIPYNLRKGF
                     GLPTAEYHDAISAYNFYRLISGANRLYFLFDSRSEGSQTGEVSRYIHQLRFQYGVKFR
                     EINVAYDINTPPETGIVVKKTPEIMAKLQSFLSSDDTGMALSATSLNSYINCPLQFYL
                     TQVEQMREPEEVQETIEAGMFGTLLHAVMENLYKPFEGKMVEKSDLEQLYSTNLTIDR
                     AIRSAFSEKYFLKGKNSETELEGNYLLVARVIRKYVKKIIETDTANTPFTYLQAEKQV
                     KIQFSIYGGKQKVNLKGFIDRVDEREGLVRVLDYKTGNGSLDFKSQDSVFDGNDEKCP
                     KFVLQTFLYCLLLQQTNNYRDIVPEILYIRNLFKPDFTTRLIDKSMPKDPKNVDNFND
                     YKEKFIENLTACLEEIFNPEVPFSQCTNMKNCEYCPYKVICRR"
     gene            24069..24767
                     /locus_tag="PKH58_02120"
     CDS             24069..24767
                     /locus_tag="PKH58_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNX87853.1"
                     /translation="MIGTIVNTASVIAGGCIGLMLKKGMPERIKTIYFQAVGIFTLAI
                     GISMVWNMEHILVIVSSLVTGAILGEWWNIEKSTEQLSEWTKKKLHIGSEKFSEGLTT
                     SFLLFCIGAMTIMGAIEEGTGGTSRLLFTKSVMDGFSAVILASAFGAGVVFSAVPLLI
                     FQGGLTLLARYASDLLTPEMIKALTSLGGILLIGLGINILEIKKLRIMNMLPALVVVV
                     IFMWIIVKFNIRLL"
     gene            24764..25204
                     /locus_tag="PKH58_02125"
     CDS             24764..25204
                     /locus_tag="PKH58_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87854.1"
                     /translation="MNVKFALIWFLVGFVLVNALKILHTILSSRKKFNRNSQKDIPVS
                     AYYKQAYFNTLKFQPFYYVIVWLTCSYIYFTTHISNNLFTDALVTGISWWALTAIAEM
                     LIWVLVNHKMNLTWKEMYLQSQPWISLTYYSVLVSPLVLSLILH"
     gene            complement(25206..26495)
                     /locus_tag="PKH58_02130"
     CDS             complement(25206..26495)
                     /locus_tag="PKH58_02130"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNX87855.1"
                     /translation="MTYQESIEYLYNRLPVFHHIGAAAYKPGLENSIRLMEQLGNPQH
                     EYKVIHVAGTNGKGSVSHFLSAILQSAGYKTGLFTSPHLVDFGERIRVDGEKINQDYV
                     AGFVENNQKVFDEIQPSFFEATMALAFRYFADCKVDVAVVEVGLGGRLDSTNIVKPEL
                     SVITNISFDHTEFLGDTLAQIAAEKAGIIKQHTPVVIGEYLPETKSVFIEKAEIIQAP
                     LIFAEDKYKVEYDDYEQNVMIVNAPGFNELRIGLGGIYQLKNIATVITATEELQKLGF
                     AIYEEDIRTGLKEVVALTNLQGRWQIVQQNPVVVLDTGHNKGGFEYITQQLKRQQCEK
                     MRIVIGMVKDKDITGMLRLLPVKAVYYFTNAKTERALPAENLKEQAEKQGLRGDAFAD
                     VQKAVRAALNDASGQDFVYIGGSNFVVGEALPVLTKQ"
     gene            complement(26499..27041)
                     /locus_tag="PKH58_02135"
     CDS             complement(26499..27041)
                     /locus_tag="PKH58_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX87856.1"
                     /translation="MNTFIELVMQRQSDRAYLQRDIETDKLDRILEAGRLAPSACNGQ
                     PWKMIVIRDEKIRNEVAATTSDIIVSSNHFTRQAPVQIVIVEESSNLSSKAGSWAKSK
                     HFPHFDLGILAAHLCLAAADEGLGSCIVGWFDEKKLRKVLEIPKEKRPVLIIVVGYSA
                     QKTRQKIRKEKSQVISENKY"
     gene            27139..28470
                     /gene="tilS"
                     /locus_tag="PKH58_02140"
     CDS             27139..28470
                     /gene="tilS"
                     /locus_tag="PKH58_02140"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNX87857.1"
                     /translation="METLKKVQLFIENNQLLTPKGRVIVGVSGGVDSVVMLNILTVSG
                     YECVVAHCNFHLRGKESDRDEQFVADLAQKYGLICKKVDFDTVNYAKRHKISIEMAAR
                     DLRYDWFAGLLKEMRAEAIAVAHHSDDSIETMLINSIRGSGLRGLYGIDERNGNIIRP
                     MLSCSREEIENYAHKNNLNYVVDSTNAASDYLRNKFRNEILPALAVINPSVKVTFLEN
                     MNRVRGAWKIYDQKIREIKEEISFTKDNQLYIDLEKLKQQVDVTTVLFELLQPYHFKN
                     SIVNDIVDGLKNNSGAKFFSDTHCVLKDRIHLIVRTNKTKSDIEYSIDKETASITEPV
                     SMEFREFLKTDDFQFSRKSDIVHVDADKLKYPLILRPWRKGDTFYPFGMTSKKKVSNF
                     FIDQKINRFQKDDIWLLISDEEIIWIAGLRLDNRFRITEETRNILEIRINQ"
     gene            28652..30556
                     /gene="rho"
                     /locus_tag="PKH58_02145"
     CDS             28652..30556
                     /gene="rho"
                     /locus_tag="PKH58_02145"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNX87858.1"
                     /translation="MTNYNILQLNAKELSELKEIAVELGLKVSNSATKEKLVYEILDQ
                     QAVANAQQKATANDGQDANRRKRMRIIQKKGKDEKVYSTQPAVAEAKPQTPAQQVNKT
                     KQEKPATPSTPESPKTEETVQKQGQKKKTSAKSKKAQNIQPVTTDTPPATAAPEKENI
                     PVQPVQNEQKVKPVKKVVLLPEVVDEVVEPELPVEPEQKPEQEVKPVQTTETEPIAVI
                     AEIKTEQIPNQKPTKSAQTNQVSGQPNGNRVKFNPRQEKEKNYDFEEVLEGTGTLEIL
                     ADGYGFLRSSDYNYLSSPDDIYVSQSQIKLFGLKTGDTVKGSIRPPKEVEKYFPLVKV
                     SQINGRSPEYVRDRVPFEHLTPLFPDEKFKLNTGKNDTLSTRMVDLFCPIGKGQRGLI
                     VAQPKTGKTVLLKEIANAISANHPEVYMIILLIDERPEEVTDMARSVNAEVISSTFDE
                     PAERHVKVASMVHEKAKRMVECGQDVVILLDSITRLARAYNTVSPASGKVLSGGVDAN
                     ALHKPKRFFGAARNIENGGSLTIIATALTETGSKMDEVIFEEFKGTGNMELQLDRKLS
                     NKRIFPAVDIMASSTRREDLLLDQDTLNRMWILRRHLADMNSIEAMEFLKDRLERTEN
                     NEEFLISMNG"
     gene            complement(30651..31070)
                     /locus_tag="PKH58_02150"
     CDS             complement(30651..31070)
                     /locus_tag="PKH58_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87859.1"
                     /translation="MKFHGVRIFKLSVGNFKKGAAMTIPGIGIFVGKGMQHDTELLRH
                     EFGHILQFRKWGFWFFWHYIVSASLKSAFNQRRKNIPHMHTWTEWSANWLAYQYFDKP
                     NDWNFSRFPIAPAGESRISKPKFVASNHIFIEEWLEA"
     gene            complement(31067..31531)
                     /locus_tag="PKH58_02155"
     CDS             complement(31067..31531)
                     /locus_tag="PKH58_02155"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNX87860.1"
                     /translation="MNIAFTTPDEYVAQLPDDRREIIQKLRSILRENLPKGYHEEISY
                     GMIGYVVPHTLYPDGYQSNPKLPLPFINLASQKNYIALYHMSISAMPELLEWFQTEYP
                     KHSKSKLDMGRGCIRLKKPEDIPYELITDLARKVSVEGWIEVYERVMKRKKK"
     gene            31819..32493
                     /locus_tag="PKH58_02160"
     CDS             31819..32493
                     /locus_tag="PKH58_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87861.1"
                     /translation="MMKKILSILILALLSSHVFAQDILEYETRTFSPYWYTGINVGSN
                     LYMAEGNDFLSGNDGVRFSLRDNSGTTIRATFGRRFTPVIGVRGYAGYSSYKWLSAYH
                     HNPVVTFGSEFITADLTISLINMLEENYSLKRYDLGLFLGTGLQLRNEITSPAVAASN
                     SSLTPVGRLGLEASFLLTNYLGLSFGGELNFVNDNNNGMVAGRNYEFIPAFSVGLNYQ
                     LKAGKK"
     gene            complement(32594..33475)
                     /gene="folD"
                     /locus_tag="PKH58_02165"
     CDS             complement(32594..33475)
                     /gene="folD"
                     /locus_tag="PKH58_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNX87862.1"
                     /translation="MILIDGKVIAEQVKAEIAGEVKQMIAAGKKRPHLAVIIVGHDGG
                     SETYVASKVRSCEQVGFKSTKIEYEDTVSEAELLSKIEELNKDEDVDGFIVQLPLPKH
                     ISEQKVIEAIDYRKDVDGFHPINIGRMSIGMPCYVSATPSGIIELLKRYEIPTSGKHC
                     VVIGRSNIVGKPIASLMMQKSVPGDATVTVCHSRTGNLKEITLQADIIIAALGQPEFL
                     TADMVKDGVVIIDVGTTRVPSTETKSGFRLKGDVKFDEVAPKCSYITPVPGGVGLMTI
                     VSLLKNTLLAAKGEIYG"
     gene            complement(33579..34895)
                     /gene="ffh"
                     /locus_tag="PKH58_02170"
     CDS             complement(33579..34895)
                     /gene="ffh"
                     /locus_tag="PKH58_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNX87863.1"
                     /translation="MFESLSERLERSLKILKGEGKITEINVAETLKEVRKALLDADVN
                     FKTAKTFTESVKAKAMGQNVLTSVKPQQLMVKIVHDELTQLMGGSSVDINLKGSPAVI
                     LMSGLQGSGKTTFSGKLANLLKTKRGKNPLLVAGDVYRPAAIEQLKVLGAQLNVPVYF
                     DETSKNPVAIAEAAIKHAKQYGHDVVIVDTAGRLAVDEQMMNEITAIKKAINPQEILF
                     VVDSMTGQDAVNTAKEFNDRLNFDGVILTKLDGDTRGGAALSIRSVVDKPIKFVGIGE
                     KMDALDIFYPERMADRILGMGDIVSLVEKAQEQYDEEEARRLSKKIAKNQFDFDDFMS
                     QIQQIKKMGNIKDLASMIPGMGKQLKDIDIDDNAFKGIEAIIHSMTPLERKTPTVLNG
                     SRKSRIAAGSGTTIIEVNRLLKQFDQMSKMMKMASQQQFKQKLKRR"
     gene            35179..>36106
                     /locus_tag="PKH58_02175"
     CDS             35179..>36106
                     /locus_tag="PKH58_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNX87864.1"
                     /translation="MKKLLFLSLFCIIFCSAGKAQTASIQNIISEIQKKLVPDKRVCI
                     FEVNLIEDNSESVLKGEISDAGIHQTLISEIRQVQPEVKDSIRVLPDSSFGENYWGII
                     PLSAIFIRQKPNFGAELTTQALMGTPVKILQKKGGWLQVQTPDRYIGWTSTDNIERIN
                     QTQLNEYNRFPKAIVISHQTRVLKNPQKKSDQITEAIMGDMVVITESKSARSKYCSVT
                     LPDGKTGYVKKEDIRLMQHREVNLSANSLIKLSKTFMGLPYFWGGTTSRGMDCSGFTK
                     TVFFMHGIILPRDASQQYYCGTEVDTSAGWGNL"
BASE COUNT        10479 a         7735 c         7305 g        10487 t
ORIGIN      
        1 atccttttta cgaacgcttt taacaactga atacaccgtg tacacaatcg ccaaagccac
       61 aatgataaga accagtatga gttgtaacat ttttttcttg tgaattaatc attctgcaaa
      121 tagtttagcc ccccaacccc ctgaaggggg agttgaagag caaataatcg ggtttatttg
      181 tcatttgaat gtgacattaa cagccaacca tttataatat cagttaagtt tctcaccaat
      241 gtgtgaatta tttgtctctg ttattagttt taaacttaaa aaacagttca tttttcacat
      301 attcagtact tcttcatata aatagctttt aaccttacct gaaaaaacaa acaatcatca
      361 ataaatcctc aatatcaccc ccttcagggg gctggggggc agctctattt acacaaacaa
      421 actccccagc tgataggttg caaaagctac aatccaggca ataacagtgg tgtaaaccat
      481 ggtgaaaatt gcccaacccc aatttgcttc ctttttgatg gctgcaatga ctgccacgca
      541 cgggaaataa atcagtataa aaagcataaa accgtacgtg acaagaggcg tgaatacttt
      601 ctggcctttc agtttacccg taaggtgggt ttgcttgtgc agattggctt tcagagtccc
      661 cgaattttca tcggcctgca ggtcggcctg atagagaatg cccatggtgc ttaccacaat
      721 ttccttggca gccagtccgg tgatgatgct aacacccatt ttccagtcat atcccagcgg
      781 actgataacc ggctcaatgg catgtcccag ctgaccgata tacgattttt cctgccgttc
      841 ggcctcctgc tcgacagtca gatgtgatac ctggttctct ttttccagtt cactgagttg
      901 aggattggtt tgcacggaag caatctgcgt atcataatct ttgctgtatt ccacattacg
      961 ggggaaataa cccagcgccc agataagaat ggatgccact aaaataaccg ttcccatctt
     1021 tctgagatat tgttgtccct tatgccacat atggatagtg gtgtttttca gcgtggggat
     1081 gcggtaagga ggtaattcca tcacgaaagg cacttctttc ttggcaaagg caacgcgttt
     1141 cattaccagg gcggtaagta ccgccagcac tatgccaata acgtaaatag agaacagcac
     1201 cagcccctga ttggttggaa agattgccga tataagcagc acgtacaccg gaaggcgggc
     1261 gctgcacgac atgaacggcg tgataatcat ggttaatata cggtctttct tgttatcgag
     1321 cgtacgggta gccatgatgg cgggaacatt acagccaaag cccatcacga gcggaataaa
     1381 cgatttgccg tgcagtccta ttttgtgcat cagcttatcc atgataaacg acgcgcgtgc
     1441 catgtatccg gtgtcttcca tcagggagat aaagaaaaag aggatgagta tgttgggcaa
     1501 aaagacaatc acacctccta ccccgccgat aataccatcg accagtaaat cgtgcagtgg
     1561 accttctgcc atcgacgttt gcagccagtc atgcaacaga cttactccgg cgtcaatcca
     1621 ttccatcgga taactgccga gggtaaaggt agcctgaaac ataagccaca tcaggaacaa
     1681 aaagatggga aatccccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc
     1801 gagcaactta acggcgacgt aacgggacga caatttgtcg gttatctcgg gatttttcca
     1861 gatttcatcc tgaatttctt tcagggcttc ttcgattagc tgaccataat taatatgaat
     1921 atgccgcacg ctttcatcac ggtcttcgta cacatcaatc agcttggaga ataattcatc
     1981 aattcctttt cctttcgatg ctacggtagg gattatcgga attccgatca tttgtcccag
     2041 actgtcataa tcaaacttca cattcttctt ctcgagctcg tcgtacatgt tcaaagctat
     2101 gaccaccttg atattcatat caatcagctg cgtggtgagg aaaaggttgc gtttcaggtt
     2161 ggaggcatcc accacgttta ccacaatatc gggattgctc tcggtaatgt gtgtgcggac
     2221 ataaagttct tcgggagaat attcagtgat ggaataggtt ccgggcaaat cgtagatttt
     2281 gaaggtgtaa tcgtcctttt tcatgacggc ttctttggca tcaaccgtaa caccgctgta
     2341 attgcccacg cgttcgtgcg atccggaagc ataattgaac agggttgtct taccacagtt
     2401 ggggttaccc accaacgcaa cccgaatggt tttgcctttt ttcagcgccg agaccttgag
     2461 ggtttcttca tcaatggtac cttcgaattt tacttcggcc aggcctgcag cctcctccac
     2521 ggaaaccact tccaccaggc gtgcttcact gcgccggagc gacacgttgt atcccatgat
     2581 ttcatactct accgggtcct gcaggggagc attcttgatt accctcactt tttcaccctt
     2641 cacaaaaccc atttcggtaa tgcgcttacg gaatgctccg tgtcccagaa ctttggtaat
     2701 aatggcttca tctcccgact gaaggtctga gaggtaagtt atatctgttg tcatcaactg
     2761 tttatcttat tgtttttaaa aatcaacccc gaaacggaga aatccaacca gtgcattctt
     2821 cagtttcgtt tcggttccgg ccggattgat aatgtactga atatccggcc ttaaatagat
     2881 atatttgcaa agctggtaat gataggtcat ttcaacagct gtttcacttc ctatgccatt
     2941 accattaata cccgcatgat tcactgccaa cccgaacaga tcattctttc ggtttttgaa
     3001 taattccctg taattcattc cgatactgcc atagtaattg ttgttgtttt gttttttgga
     3061 actaagcccc agctgtgaga aaagcgacag tttatcagtt atctgctggt cggccacaaa
     3121 ataaaaacca ccgtttttaa agttgtttaa tgacgtgttg ttatgtttat gataatatgt
     3181 gcccagttta tagaccgcat cccgaccttt tattagactt ttgttgatct gaaattctgt
     3241 aacagcgagt aatccctgat cattccccag tttccaatgg atattatagg ggtttctctc
     3301 aaaatcgtcg ggtgttccat caaaaaaggc tgcctgccag ttgaatgtat cggatatcct
     3361 gaactgcaat gtcgcgccta aggcggttaa gggaaaaatg ggagaagtga tattatcggc
     3421 aatgctggaa tgaatgccaa aggaactgtt tgtaaacagc gcgccgtttt cgcttacggc
     3481 aaaattcgcg ttcaaatcct gcaagccgat tgttaaatca aatttaccaa gagtttgttt
     3541 ataccagagt tcatacataa aagtgagatt accggcctca atattggaaa ctccctgaaa
     3601 atcgccgacc aggtttgccg atggctcacc tccatgtgtg ttacctacat ttataaaaaa
     3661 ttcacctcct ttccaccagt tggctttttc tgtgtcaaac ccggctttaa tattggctaa
     3721 tcccagatag gttgttccgg ttttaatacc tccgctgaag ttactgacca aatcgccgat
     3781 gtaggatgct tcaaatgtga aaggcgacgt ttttttaacc gagtcggctt cctgagctga
     3841 aagtccggtt attgaaaatg tgctgattaa aacagctgca aatactgatt tatttattaa
     3901 tctcatatat tactttttta ttgatttctt tacggcaaaa ttactccgaa gaaaccaact
     3961 cataaatcac tatttatatc tatttcgttg caaaccatta actacaagta ggtatattta
     4021 cgtattaaat cattaacggc atcacaaaac caactctttt cagttaaaaa acttcctcat
     4081 cagtttttaa ttatttagct ttatatcatc cagttccacc agtttattca taatggcata
     4141 aatagtcaga cctgcagtac tgtgtatttc cagttttcgc gcaatgtttc gtctgtgggt
     4201 tataaccgtg tgtgcggaca gaaacaaatt ttgcgcaatt tccttattag tcattccccg
     4261 aaccacacag gcaataattt ctttttcgcg tgtgctcagt gtatttggct cttcgctatt
     4321 ttcgaccggg ggcgaactga aaagcttttc gaatttctcc cttatctgat cgactgtatc
     4381 gtatatactt atccgttcat cataatcgcg cagtaaatgc tcatcaatga cagagtaaac
     4441 aagcgcgatg caaataatcc tgccgttagt tgtttccgtt ttgattttag caatatcaaa
     4501 atatccccag aatgacggat taataattaa cacatccggc ttatgaatgt tcagatagtg
     4561 attcattgaa tcgatggtca gaatctcgac aggttgaatc cggtaacccg gtattcgttt
     4621 caaaacggcc gaaagtccac tccgcacaat taccgaaggt tctacaatag ctattttcag
     4681 tggattgccg ttcatttctt cagattgcgt ttacgttcaa cagcttcaac agcggggata
     4741 aaaagaaagt cctcgacctg actgtgtgtg gcaagatcca tttcacacga aaacacatca
     4801 aacaaaacct ctgtcagcag cggattgttg cccttgtcgg gatagtattt cacgagtata
     4861 tttttcaact ctacgagttt agattcaatt tcattgtgcc gatgcctgaa aactgatatg
     4921 ttgtagatca gtcccggttc gtcattcaac aaccggataa catacggaaa agcaacttta
     4981 tcttcatatt ccatgtgttt tcggacttcc gatacatatt cgtcgaagaa tttcagaaaa
     5041 acggtaaggt atgggacatt ctgatcatga ctgctgacag cctcaatcag tttattacga
     5101 atttgaggta atttgaattc gagaaaatat acatgagcat tcttgaggta attaatgagt
     5161 gacccgatgg aaatatcttc gtaatcatga tccacttcaa agtttgcttc agtgaggaaa
     5221 ttaacaacag ccagaaaagt acggcaatca attccgtttt ccgagcagac ttgtttcacc
     5281 gaatggtcgc caaaacccaa cgacaatccg aaacggctga ttaccagcaa cagggaatga
     5341 ttctcgttta tcagatcact catcttgtct gtttccgaaa atttatgctg tttaaacatg
     5401 ggcgtgatgt attttacagg attatttcaa cgaaaaaaag aataaacttc cccgaaacag
     5461 atggctgttg tcccgacaga agtttatact tactaattta ctaacactta gtctatatgg
     5521 ttatatatta cattgcaaag atcagactat ttccgcaggc ttgcaatcac catatttagg
     5581 tattttcggg ctacaaaacc aaaactgcgg tatttctgtt ccatttaagt tttcagccac
     5641 aaaggtagta atttataacg tttctaaaaa caacaaggga ttattataga acaaatgagt
     5701 ttgtgctaat atctcccgtt atgtttttat tctacctgtt caatgattta taggcatcga
     5761 gcagctggcg cgcagctaca aacgaactta cttcatcagt cagcacccgc ctttcggctt
     5821 cttcgaggag gatttgaatc ttatcgttgt tgtaaaagcc cgatttaagg tggtcattga
     5881 tggtttcgta catccagtat ttggcctgat cggtgcgtct gatctcaaaa aatccattgt
     5941 cacggacaaa accgatgtac ctgagcacca tttcccatac ctctgctatt cccgatcgtt
     6001 ccattgccga acaggtaacg acatcgggtt tccatcccga ttcgggcagg ggaaacagat
     6061 gcagcgcgtt aagatattgt cgccgcgcca actgcgattt ttcaatgttt gtaccgtcgc
     6121 atttattaat ggcaatgcca tctgccattt ccataatacc acgcttaata ccctggagtt
     6181 cgtcgccggt acctgccagc tgaataagca aaaagaaatc gaccatggaa tgtacagccg
     6241 tttcggactg ccccactccc actgtttcca caaaaaccac atcgaaacct gctgcttcgc
     6301 acaggataat gctttcacgt gttttgcggg caacaccacc cagcgaaccc gctgaaggtg
     6361 aagggcggat aaaggcattc tttgccacgg aaagttcttc catgcgggtt ttatcaccca
     6421 ggatacttcc cttggtacgc tcgctgctcg ggtcgatggc aagtacggcc agtttcttcc
     6481 cgtcatggat cagatgcatg cccagcgctt cgatgaaagt acttttacct gcgcccggaa
     6541 ctcccgtaat acccaggcgg acggacttac cggcataagg cagacatttc tcaatcactt
     6601 cctgagctat ttcctgatgc tgcgacaaag aactttccac aagcgtaacc gcctgaccca
     6661 gtatggagat atcgcccctg aggatacctt ccacgtattc ggtcgtagtt aacagctgac
     6721 gttttatgcg gggcttcgcg tacggattga tagtcggaac atccgatact ccttcgttaa
     6781 cggacaatcc tttatagttc ggatcgtttt ccggatggtg gtgacgatag tgcataatgt
     6841 taaattttac cggatttaga ataagaaatc gcctggcatc gcatatgatg ggcagacgat
     6901 ttcaatcaaa aattatagtt ttcaatgaca gtttatttct ttactgacca gctaatggtg
     6961 tatgtcataa aactgttacc agggtcgttc gtctgtatta tgaacgattt tttataatcg
     7021 ccaacaggaa atgctttcgt gtcaatatca attttgagtt ctccggtttt accccccctg
     7081 attgataatt ttcccggatg tacagtgatg ttgctgttgt tgttgatgac acggcggatt
     7141 tcaagtgctt tctgaccgct gtttttaata attgctttac cacgtactat actgccctgt
     7201 ttcatggagc caagtatcag gttgggtgat ttgatttcca gaatgggtgc ctgacgtttt
     7261 tcatctattg tcatcttgct gaaatcatcc atcactgtag cgtttatagt aattttgtgc
     7321 gcatcgtcag caactttctt gccattaagc accagtagta cgttatcata aacaggaccc
     7381 cattcggcac attttttaga atcgaataca aactctattt tgccttcctc gttcgggttg
     7441 agaacgtctg gttttgcctc agctttgagg taaacaggca ggtcactgac agcaaccgtg
     7501 agttttgtgt tcgaaccgtt ttttaccagc aatgtattgg ttctggtgct tcccttagtc
     7561 acattactca tttgtatcat ctttgttctc aacttcagat cgcctgcttg aacaggataa
     7621 ttttctgcag ctgctgtcga tttggggttc actgtacctt ttattatcag actttccact
     7681 tcgttgctgg cgttgctgta aaccgtgata tttttagtaa atgcgccggg acgacctgcc
     7741 gggttgtagg ttacagtgat ttcacctttt ttaccaggtt caatcggtgt tttggtccat
     7801 tcgggagtgg tacatccaca cgatgcattt acccgttgca caagcaatgc ggtactaccg
     7861 ctattggtaa attcaaatac atgagtagct tttccgtctg attcattgat agttccgaaa
     7921 tcaaaggttt tctcttcaaa agtaatggtc ggtttctgtc ccatgacagc gatggttgtg
     7981 aggactgaaa tgatcactaa aaatccaatt cttttaatca tatggtttat tattttttag
     8041 tttaacaagt aaatttcatt gaaccgtgca aaattataaa aaatgtcaat gccatgtcca
     8101 taaaactttg aaaactttat cagttctatt taaataaagg ttaaataaaa aacaaaaatg
     8161 aagtatattt gcatctgtat ttcttttaaa aatcgtgctt taacagcaca atttaagcta
     8221 tgctgaaaaa atttaaaaat tctgctctgt ctgtattcga ctatactaca gcctgttacc
     8281 gacaccttca tactcatcct gaactttctt ttcaggaagt tgctacttca gcttttatcc
     8341 aaaaacagtt gacagacctt gatatacctt tccgtgcaaa cgttgcaggt acaggtgttc
     8401 tgggacgtat tgaaggcaaa aatcctcaga aaaaattgat tgctctgcga gctgatatgg
     8461 atgctctgcc ggttcaggaa gcggtggata ttccctggaa atcgctcaat tcaaatataa
     8521 tgcatgcctg cggtcatgat gcccatgttg cctgtctgct gggtgctgca aaaattctta
     8581 aggaacattg cagtgaattt gagggtacca tattactcgt cttccagccc ggcgaagaaa
     8641 aagctcccgg aggtgcttca ctgatgctgc aggatggaat ctttgatgaa tataaacccg
     8701 agttcattct gggacaacat gtatctgtcg attttcctgt cggtacaatg gcattcctgc
     8761 ccggcacgat catggcatct gctgatgaaa ttcacctgac aatccatggc aagggtggtc
     8821 atggagcttt gccacatctg tgcaacgaca cagtactggc agcatcgcaa acagtagttg
     8881 cgctgcaaca ggtacgaagc cgtctctgtc atccgcttac accgatggtt ttgactttcg
     8941 gaaaactgat tgctgatggt gcgacgaata tcattccttc ggaagtaaag ctttcgggca
     9001 ctttccgtac ggtggatgaa acgtggcgca gcgaaggtct gaagcatata cgacgcatca
     9061 ttacagaaac ctgtgcggca tcgggttgca gcgtggatat tgatattcct tcgggttatc
     9121 cctgcgttat caataactct gagcttacgc tgaaagccgg acagtttgcc gcggaatggg
     9181 caggcgcaga gcatatcagt caactggaat tgcgcatgac ttccgaagat tttggtttct
     9241 tctcacagca atttccgagt tgcttttacc gattcggggt aaaaggaacc agcaatcaga
     9301 actgcggagg cttgcacagt ccaactttct gcatcgacga aaatgccctg ctgaccggtg
     9361 ccggcggact tgcctggatt gccttcagac ttatatccta aagctcaacc ttatacactc
     9421 tccggtagat ggtggatttg atcttttcga aattttcaat tacaaaatcc acctgatcag
     9481 tcataagaaa caagggctga tagtaaccat tgcggttgta tggtggttgc tggtagtcaa
     9541 caggaacaac ctcatagccc agtcgccgat agaattcaat tcggcgtgca gccatttcct
     9601 cgtcgggcag ttcaacttca aaaaccatag gaatatccga ctgaggtttc atcatattca
     9661 tgaccttttc gcccagtttg tttccccgaa tggcttcgag aatggcaaaa tgttcggcat
     9721 atataaacca gtcaaagtcc caatagttaa aaaatcccac gaagttatcg ttcattaata
     9781 cggcattgca gtaaaaatcc ggctcactgc cgagcaatgc cttgaagtct tcttccgtgt
     9841 ggcgctgatt ttccggaaat gcctcctgat aaagtggcat tagcgaatgt atgtgttcat
     9901 catcagccga tttgatttga attatttcca tgttgaacgg tgctggttta taaattctga
     9961 ttgattattt caacccttcg ttttaacttt caaaattaaa gccaaaaaag ggtttctgaa
    10021 aatatttacc gaaatatttt tgccttagat gaaaaatatt gcggcatgat gacaattcaa
    10081 aaaaaacggt ttcacttcat aaagcgaaac cgtttatatt tataaaaatg ctgataatta
    10141 attgcatccg caaccgcaac cgtgattatc gtcacagtcg tcaccgcaac cgcatccgcc
    10201 gtcgccgcaa ctgcaaccac ctcctaataa tgatttcagt tcctgttcgg ttgcatcgcg
    10261 cacacccaga acttttccct tgaaatgaag agtttcacca gccaggggat gattcagatc
    10321 cacagttaca tgtgtatcgg taaccgacac cacctgtgcc tgcagacggt ttccttcgtt
    10381 atccataagc ggtactatgt tgccttcgaa aaccatttcc tcatccagtt ttccgtcaat
    10441 ttcaaaaaca gcacgttcca gttccacaac tgcatcatcg tcatattcgc cataagcatc
    10501 ttcgttcttt atggtaaatt caaagttatc atctttttga agaccgttca ggttttcttc
    10561 aaatttaggc agcatcatcc ccacaccata tataaagttc aatggctgtt cgggagtagc
    10621 tctttccatc aattccggtt ctccgttttc gccatcaacg tacagttcgt attcggcatc
    10681 tactacttta ttaatttcaa ttttcatttg tttgttttgt ttgttattat tgattgattt
    10741 atttttctac agatttatat aactcaatac caggtaatat tgttcggtga acttcgattt
    10801 ccctgtttct catacttcag atcctgcaac atactggaat ttacaccaat gcggaaatta
    10861 taggttttat aaggtccgaa aggcactaca ttaccgctga gggtccagca gtgaagatta
    10921 cgggtgatat tgaaactggt gtatgacatt tgtttggcat tgaaatcata ggaagtcgta
    10981 ccgcttatct gccagcccgg tgtgagtttt atattcccgg ttatacccag attatgggtg
    11041 aaaacctgtt tgtattcgag tttttcgtag ttgaattccg atgagtttcc aaacctcact
    11101 gaatagttga aactcaggct ccaggggatt tccacctttt cgtatccgtc ggcatcggtc
    11161 gttgttttat cagttccgga attgctgtct ttttgctgag atctcatgct atccgcattt
    11221 atatctgagt tgcccgtatc ttcatccgac tcagtatttg tgttgccttt gcttttttta
    11281 tcctttcgct tgaatgtttc gttcgaaagt gtatagctgt aacttgtact tgttccgagg
    11341 aatcgaggga atttaccatg ttcccaacgc aactgattga cacgtttgat gctttttcca
    11401 tcgggtgaga catacatata cggatcgaaa gcgccactta agctcagggt atatgcttta
    11461 ccgaatttaa tacgcagaga tgtaccgata ttagaccatt gcatggagtc tgcagcaaag
    11521 ttataggcgc tgttcagcga gaaattatct atcagactta tgattttcgt tggcttcaaa
    11581 ccggttgtat cctcgttatt tttcactttc atttccaggt tattatccaa agtaaaggaa
    11641 aggttgttgg attttccacg accgggagct ccgctcggga agcgcgaata gtaaatctct
    11701 tcggaggtaa tcttggtcgg atcaccatct tttataacag atttgatata ggtatcccag
    11761 tatccatatt tcagatcgcc aaagtcgggt gtaaaagcaa aacttgcact gggcttgagc
    11821 atgtggcgga ttttttctat tttatcgcca aaaattgctt tcacaggagt gtacattcca
    11881 tacaaggttg tattggcaga catactgagg ttgtaatccc acacacggtt gaaaccggtc
    11941 acattggtgg ttacttcttt ctgattgact acatcccagg tttttctctg agatgtaagg
    12001 aaccattttt ccttgtagtt aaacgatggg gtaaatgtaa tgtatttcag gacattgaac
    12061 gatgcggaaa caggtatatt gtgctgtgcc gaattgaccc agtcctttgc taaagacgaa
    12121 tgcatcagtt tgttttcttt cgtctgaata ctgttggtta tagttcctga gtacgacatc
    12181 gagatttttt cgtaccatct ttcatttcct accggatttt ttcgtttcag gggataaata
    12241 cgactcatac tgatagccac attgggcagc ataaggttga tggtagaatc tttggttcgc
    12301 tgcgttaccg acatattggc tgtaatgctg aacggattgt tcggaaaccg ttgtgagaat
    12361 gatacgcttg aacttttagt attttcagcg ttggcgggag tatagtaact gttgatatta
    12421 ctccggttcc atccgctggt agtaaagttg acactggccg aaaaggtccg gaacggatta
    12481 actttcggat tctgtgaatg cgaccattga atgcttaaat tcgggctttt ctggtaatcg
    12541 ggcatatcct tttctccaat tacatcctcc cgatagctga catttacact tccattgaat
    12601 ttataccttt tcagatagct ggatgacagg ttaacgcccc aggttccacg ggtataaacg
    12661 tcgcctttaa tgctcaaatc ggcataatcg ctgatggcaa aatagtagcc cccattaact
    12721 aatccgtacc cgcgtgttac gtcagtatcg aaactcggca tttccaaacc ggaggaataa
    12781 ttcttattga agggaaaaaa accgaaagga acagcaacag gtagaggaat atccaaaagt
    12841 accaaatgag ccggacctgt aatgatgtaa cttcccggtt tcacttttgc tttactcagg
    12901 cttaggtaaa agtgtgggtg ttcatggttg tcgcaggtag tgtacatgcc gtcctgcata
    12961 caaagtacat cgtcatcgtt ttttttggtt ttatcgctga tgatataacc ttcaccctgc
    13021 tgggttaccg cctgacgtat aaaacctttc cgggtcctta ggttgtaggt aagttctgtg
    13081 gcattgtatt cctggtcacc gtcattaatg accggcttgc ctttcataac acccagacta
    13141 tcgggtacgc cccgcgcaaa aattgtactg ctgtctgtac tgatgcgcac aaagtcagct
    13201 tctttcagat taattttttt atagctgaga ttggttttcc cgtataaaaa gccggtaccg
    13261 tcacccagaa agacgatgga gtccgaagct gtatatgcaa tttcggcatc tatctgattt
    13321 gggttaacgg gtttggtcag cttactgctg tcattgaccg aggcaacttt ctgactatcg
    13381 gttggcagta aattgttttt agaattatca aatggcgtgg acttttgccc tgacgcatgc
    13441 gttgcatacg taaaacaagc cagcaaaaga aataaaaccg taagttttgt gttatataat
    13501 tgtttactgc taagcattcg gtacaggttc gtcaaaaaaa acaagttgca aatttaagta
    13561 aaaaaagagt gtgcttatgc ttttattttc aattttatga ataatttgat attttacaat
    13621 ttattaaatg cctcaacact ggataaaatt actacttttg caagcaatta caatgtattt
    13681 atgaactcat ctttattttt tcggaaaaca atgaaatatc ttcgctacac tacatttatt
    13741 ataatactta ttttaacgtg tattgtatct ccttcatatg ctcaaaaaaa atttaccgtt
    13801 gtaattgacg ccggtcacgg cggacatgat tccggcgcgg ttggccggta ttcgaaagaa
    13861 aaaaacataa accttgctgt tgcgctttca ctgggcagta aaattgaagc aagttacccc
    13921 gatgttaaag ttgtttacac acgtgaccag gatatttttc ttaccttgca ggagcgtgcc
    13981 gatattgcga acaacaataa cgccgatatc tttttctgca tccataccaa tgctaataaa
    14041 tcatctaccg ctttcggggc tgaaacatac acgctgggat tgcataaatc gcagtcgaat
    14101 cttgatgtag ccatgcgcga aaactcggtt atcaccctgg aagaggacta taaagcacga
    14161 tatcagggat ttgatcccag tgctcccgac acatacatca tgtttgaatt catgcaggac
    14221 aaatatatag acaaaagtat tgagctggca tctgctatcc aaaatcaatt tgtacagaca
    14281 agaaggtatg accgcggcgt acgtcaggct ggtttctggg tacttgccaa atcggcatgt
    14341 ccgagcgtgc tggttgaact tggttttatt tcgaaccgcg ctgaagaaga ttatctgaac
    14401 accagcgaag gacgcgacga aatggcaaac tcaattttcc gagctttcga attatacaaa
    14461 cgtgaacacg ataaaaaatc gggtatcaaa gttacacctt tgaagaaagt agacctaccg
    14521 acaccgtata aggaggtgga aaaaccggtt aaaactgaaa caccggtact tgctgcagta
    14581 tctactaaaa ccgacactac aaaagagctt aaaatccgtc ccgaaacgga taaaaccatc
    14641 cagacggtaa cgaaacctga cgaagcaaaa aagacaaacg aagtagtttt caaaattcag
    14701 ctttttgctc tggagaaaaa actgaaaacg aatgcaccgg atttcaaagg tcttaaaaat
    14761 gcagatttct atgttgaaaa tcaactctat aaatacacct atggtgaaga atctaacttc
    14821 agcgaaataa ataaactccg gaaaagtatt tcaaagaaat ttccgaatgc ctacgtaatt
    14881 gccttcagaa acggtgaaaa gatctccgta aaggaagccc tgaagaaata aaaccagact
    14941 ctcttcaaaa ataaaaattc actcaatgaa aaaaaatatc tttacccgcg aagtaaaagt
    15001 gggtttaatg gttattgctg ctatatttat cctatacttt ggcctgaatt ttcttaaagg
    15061 aatagacatt ttccagaaaa caaatttata ctatggtcaa tatgaggata ttggtggttt
    15121 ggttccctcg gcaccggtct atatcaaagg atacaaagca ggtcaggtgg aagaagtaaa
    15181 atatgatttc tctaaaaagg aatctttttt agtgaaaata tccataacca gcgatatcaa
    15241 attacctgag ggcactataa tgcagctggc tgacgaagga ctgatgggtg gaaaagtaat
    15301 tcagctcatc tatgctccgg ttactgccgg tcagaaaatg cataagccac atgatattct
    15361 gccgactgaa gtgagcaccg gactgatggc aaccgtagcg ggagatctga tgcctaaaat
    15421 tgaaaatgta gctactcaag cagactcgct tcttcgttct gtcagaacac tgatcgaaag
    15481 gaaggaaata agcaacagcc tgtcatctat cgaaaaaacg actgccgatc tatctgcatc
    15541 atcggctcag ctcaaaggac tgctcaacaa tcagctgccc tccatcctga atgatgtgaa
    15601 tactgtcaca ggtgacttca aacagcttag tggcaatctt agaaatatcg attttgcaaa
    15661 aacggtaaac agcgtggatt atacattgaa aaaccttcag acaatcactg ataaaataaa
    15721 cagcaatgaa agcagtcttg gtttactcat gaatgataaa acgctgtata tcaatctgtc
    15781 gaacactgca aatgccgccg acaagttgct tatcgattta aaagaaaatc ccaaaagata
    15841 tgtaagtttt tctttaatcg gtaataataa aaagtaataa tttttgcttt cccacacaat
    15901 ctcatattta gaaattatat aaataacaaa aaaattgaaa tatttttttt cttcaagaaa
    15961 tctacaaatt ttcaagtctt actgcatttt cagaaaaaaa cattcaattt taacattaaa
    16021 ttgttaatct gttaataatc aagcgaattg aattaactaa aaatcaaaat acattgacat
    16081 acaatcgatt acactagagt actgaaagac aaagagttaa aacaataaaa aaatttattg
    16141 aaataacaga tttgtttatt caaaaaaagt ttctgaaatt tgtaaaactg aaatgcgaca
    16201 taagctgttc ggtttagatt acatattata aacaacttac taactcggct tcggtcgagt
    16261 tttttatcct aaatttattc tgttaaatta aaaatgagtt atcactacca gttgtggaat
    16321 agttgcctga acattattaa ggacaatgta aatgaatcat cctttaatac atggtttgct
    16381 cctattgtac cagttaaatt cgaaaataag atttttgtac ttcaggttcc aagtcagttt
    16441 ttcgtggaat acattgaaga aaaatacatt gatctgctca gaatgtcact gacacgtgtt
    16501 gctggtaacg gaacaaaact ggaataccgg gtactcatcg acaaatcaag cggcacagga
    16561 acccagatac ccagtacagc cgagacttcc aaacctgttc agacaaatta cagttcgcag
    16621 caaaatgcaa tcgtgtcgcc gtaccagcgg ttacagttgc ctgaaattga tccgcaactc
    16681 aatccttcgt acaatttttc cagtctgatt gaaggccgca gcaataaact ggcccgaaca
    16741 gccggtatga gcattgccaa cgatcccgga aaaacgattt tcaatccgct gtttatgtac
    16801 ggaaaatcag gagttggaaa aacgcacctg gccaatgcca tcggggtgat gaccaaacaa
    16861 caattcccgg ggaaaagagt gctctatgta tctgccaaca cattcctgat ccaatacacg
    16921 gatgctgtca ggaacaacac acagaatgat tttctgaact tctatcagac tatcgatgta
    16981 ctgataatgg acgacattca ggagtttgcc ggtaaaactg ccacacagaa tactttcttc
    17041 catattttca atcacctgca tcagaccggt aaacagctgg tacttacagc cgaccgttct
    17101 cccctcgaga tggcaggcct ggagcaacga ttgcttaccc gtttcaaatg ggggctttca
    17161 gccgaaatag agaaaccgga ttatgaactc cgtaaatcta tactggaaag caaaatttac
    17221 agagacggac tggaaatacc tgaaaatgtt gttgattaca ttgctgaaaa cgtagtagat
    17281 aatgtacgcg acctggaagg tgtcctgatc tctttactgg cacattccac acttacgaat
    17341 atgcctatcg atatccgcct ggcagaaaaa ataatcagcc gcatagtcaa tatcacccct
    17401 aaagtgacta cgattgaaca aatccgcgaa gttgtttgcg attacttcaa actctcggta
    17461 gatgcaattt caacaaaatc gcgtaagctg gaagttgttc aggcaagaca gatagccatg
    17521 tatctgtcga aaaatatgac taagaattca ttgtcaatga ttggtaacta tatcggtcag
    17581 cgcgatcacg ctaccgtact gcatgcctgc aagaaagtag ttgacctgat ggacacagac
    17641 aaacatttca ggaaaagtgt tgaagagatt gaagaaagac tgaagaaata agaacaaaca
    17701 agaaataata caaagacaag acctgcattt agaaatggag gtcttttttt atgaatttta
    17761 ttcaacaaat ccttctgcca aacaaaaaaa tattgtattt ttacaaccgt aattattcaa
    17821 cacttaatag ctatgatatt taaattcaca atcatatcag acgagaatga cgatttcctg
    17881 agaatcatag acatcgattc cgaagcatcg tttctggacc ttcataatgc catccttgaa
    17941 gctgttgatt ttgaaaaaaa tcagatgacc tcattcttct tatgcaatga cgactgggaa
    18001 aaagaacagg aaattaccct ggtagagatg gaatcgagtt ccgaatatga caatctggtg
    18061 atggaagata ctttcctcga ccaaatactg actgatgaaa aacaaaagtt gttatttgta
    18121 tttgacatga tatcagaccg cgcattcttc atggaactca cggaaatgat acccggaaaa
    18181 gatataaacg aagctgtctg tgtaacttca acaggcttgc cacccgtaca aatagcagat
    18241 gatactgatt ttatcacgcc gcaaaaaccg aacctctctt ccgaagaaag tttctacggt
    18301 gatgaagatt ttgatattga cgaactggat gaagaaggat ttggcgattt aaattttgat
    18361 gaaagcagct tatttaccga cgataatcat taatatcaac caataagata caataaagac
    18421 aggaaatatt tcagcaatga agtgttttct gtcttgtttt tttgataatg agcaccctga
    18481 ttgtcataac cggaccaacc ggtgttggga aaacaaatat cagcctgcaa ctggccgaaa
    18541 agtttggttg tccgattatc tcggccgact cgcgtcagtt ttaccgtgaa ctgaaaatcg
    18601 gaacagctgc gccctctgaa gaggaacttc agcgtgttac acactatttc atcggtactc
    18661 attctatatt cgatgaatac aatgcaggac agtatgaaga ggatgtcatt gttttactgg
    18721 acgaattgtt tcagaaacat tcagtggtac tacttgtggg tggatctatg atgtacgtgg
    18781 atgcggtgtg caacggactg gacaatattc ctactgtaga tgtagaaacc cgccggttct
    18841 ggcaaaagga atatgctgca aaagggctgg aatttattca gaacgagtta agaaggctgg
    18901 acgaaaagca ttacaacgag gtagatctta aaaatccgaa acgtatcctg catgcccttg
    18961 aaatatgcac catgacaggt aatccctact ccaacctgcg caccggagaa cgaaagaaga
    19021 gaaatttcga aattctgaaa attggactga atttgcccag acctgaactt tacgaaagga
    19081 ttaaccggcg cgttgaccag atgatggaag ccggtttgct cgaagaggct tcgcagtttt
    19141 atccacacaa gcatctgaat acgctgaata ctgtaggcta caaggagatt tatgctttta
    19201 tggatggaga atgggatatc gaaacggcaa tcggtatgat taaacaaaac agcagacgtt
    19261 atgctaaaag acaactgacc tggtataacc gcgatgctga gatcaactgg tttcgtccgg
    19321 atgacgaaaa aagtattatg gatttaatta cctctcagct tcagataaaa gataacggac
    19381 cgataataaa ctgacacaca aaaattgatg gaaatccgac aaaacatatc gctcaagccc
    19441 tataacacct tcggcatcga tgtaaaggca aaatactttg ccgagtacaa ttcggtgaca
    19501 gaactcaaag agctgatcag ttctgaagtt ttttgttcca accgacattt tcacatcggt
    19561 ggtggaagta atttattact tctgaatgat tatgacggac ttatccttca ttcgcagatt
    19621 aaagaaattg aaaaaaccgg tgaggatgaa gaatttgtct ttctgcgtgt cggttcagga
    19681 atggtttggg acgattttgt agcttattgt gtatccaata attattacgg tgttgaaaat
    19741 ctgtcgctga taccgggtga agtcggcgct tcggccgttc agaatatcgg tgcatatggt
    19801 atggaagtga aagacgcaat cttcagtgtg gaaactatag aaacagcaac tttacaaaac
    19861 cggatattca gccgggaaga atgccggtat ggctaccgga aaagcatttt caaggaggaa
    19921 cttaaaggcc aatatattat cactcatgtt acgttcagat taaagaaaaa tcccgattat
    19981 cagcttgact accagcacct gaaacaggct gtattagaac gcggagacat caacctgaac
    20041 aacatccggg aaactgtcat atccattcgt caaagcaagc ttcctgatcc tgcgatcagc
    20101 ggtaacgcgg gtagcttctt tatgaatccg gtgatttcgg cagaaaaatt cgaaatgctt
    20161 caacaaattt acccgaccat gccccactat ccggtttcac aatcggaagt aaaagttccg
    20221 gctggctggc tgattgagca atccggattg aaaggaaaat cgtttggtcg tgtgggtgta
    20281 catagcaaac aggcattagt aatcatcaat ttgggaaatg ccactggtca ggaagttgcc
    20341 gatctggccg ccatgattat ggaaaatgtt tggcaaaagt tcggtattga aatccgaccg
    20401 gaggtgaatt ttatttgatc aggatgaaat aatctgaaaa agatcttacg agccactacg
    20461 ccagttcctt tatttccagg ttttataagg ctgtatggat aaataagaat tataatatct
    20521 tctgtcgcct gtaacttcag tacccagcca gtccggtttt gtgtagcttt cgtcctctga
    20581 ctgcagttcg agttctgcga gtaaaaggcc ttcattttca ccaaaaaact catccacctc
    20641 aaagattttt tcgtcatatt ccacttcatg acgcacctta tcaatcatac ccttttggca
    20701 tagcaacaaa aggttctcag catcttcctt tgtaatttcc ttttcccatt cgaaccggct
    20761 gatacccgta ttacctgttt tccccttgat ggtgataaat cccttgtctg cttttatccg
    20821 gatgcgaacc gtacgttcgg gcgaagcgca aagatagcct tgggtaattc tgaaactacg
    20881 cgaaacaaat ggtctgaagt ctcccctgac cagaaatttg cgttcgatct ctatgttgtc
    20941 tgattttttc ataccaattt aataatttag ttatttatct tcggcaaatt accttgtagg
    21001 gacaatattc acaatttttc atgtttgtac attgcgaaaa cggaacctcc ggattgaaaa
    21061 tttcttccaa acaggctgta agattttcta taaatttctc tttgtaatca ttgaaattat
    21121 ccacgttttt tgggtcttta ggcatagact tgtcgatcag tcgtgtagta aaatccggct
    21181 tgaaaagatt gcggatatac agtatttcgg gaacgatgtc ccgataattg ttagtctgtt
    21241 gtaaaaggag acagtacaga aaagtctgaa gaacaaattt agggcatttt tcatcatttc
    21301 catcgaaaac gctatcctgt gatttgaaat ccagactgcc atttcctgtt ttatagtcta
    21361 ggacccgcac caacccttcg cgttcgtcca cccggtcaat aaaacctttc agatttactt
    21421 tctgtttacc tccataaatc gagaactgga ttttcacctg tttttcagct tgaagataag
    21481 tgaaaggtgt attcgcagta tctgtttcga tgattttttt cacatatttc cttattacac
    21541 gagccacgag caggtaattg ccttccagtt ctgtttcgga attttttcct ttcagaaaat
    21601 atttttcgga gaaagcggag cgtatcgcgc ggtcgatggt cagattggta gagtaaagct
    21661 gttccagatc agatttttcc accattttac cttcgaaagg tttgtacagg ttttccatca
    21721 ccgcgtgaag caaagttccg aacattcctg cttcaatggt ttcctgcacc tcttccggtt
    21781 cgcgcatttg ctctacctga gtaaggtaaa actgcaacgg acagttgata tagctgttca
    21841 gcgaagtagc agaaagagcc attccggtgt catcggaact cagaaacgac tgaagttttg
    21901 ccataatttc aggcgttttt tttaccacaa tgcctgtttc gggcggcgtg ttgatatcat
    21961 acgccacatt gatttcccta aatttaactc catactgaaa gcgaagctga tgaatgtacc
    22021 ggcttacttc accggtctga cttccttcgg aacggctgtc gaaaagaaaa tacaagcggt
    22081 tggctcctga aatgagtctg tagaaattgt atgcggaaat tgcatcgtga tattcggcag
    22141 tgggtaaacc aaagcctttt cgcaggttgt aggggataaa actgtttgaa gtgttttttt
    22201 tgggaaagac accttcgttg aaagaacaga ttatcagatt atcgaaatca agcccacggg
    22261 tttcgagcat tcccataatt tgcaatccgt caagcggttc gccctcaaat ggtattgaaa
    22321 ttcccgccgt aagctgacgt atgagacgca tgatcgtatc cagactgatt tctgtgtcag
    22381 cagctgtttc cgcaattaca gttttcaacc ggttaagagc aagataatac tgatgcaaaa
    22441 aatcacattc cagcctgtag gcatttttct cattggcttt ttgctcccag gcaatgttca
    22501 gtttcttcag gatttccagc aggtaatcga taaaaccgtc tgatgtgttg atgggcttaa
    22561 agattgcagt cagcaaatcg tttccgttaa aggcatcttt cccgacatac atggaatttg
    22621 tcctgatcat catttcatca attccgcgcg ctttttcgct gcaatgcaga tgtatatact
    22681 ggtggttcag aatgtttgaa acggtgcggt gataaaacat ctggtgctta ttcatccgtc
    22741 tttgcagttc gaagatattg tccagtaatc ccgaaacagg tgtagcattc agcggaaaac
    22801 ccatggtcac attgattttt tccacattcg gaggcatgga atgaagcata ggtatcagca
    22861 gattttcatc gggtaataca actgctgttt ttatccattc cggactttcg ttgttttccg
    22921 gtaatatatt ttgcagcaaa cggtacacct ctttggtctg tcccactgct gaaggaatgg
    22981 agatcagttc gaacgattta caacttaatt cggagttact tggttctatt tttaatggtc
    23041 cggtaaaaaa atgaacattt tccctgtaga aaagtgaggc ctgattttgg tcatcgcgca
    23101 actccgaagc ttcataatcc cagtaaaaat ctgcctgtcc gcgtttctgt agttcagcaa
    23161 aaagcattcg ttcacaagga tttaaggcat taaatccgac aaaaacaaat tttttaccgg
    23221 cgaaaaactc cggatcttct tttcgtttca actgttcggc tatttcgcgg aaaatcatcc
    23281 cttctgtagc tgtattctcg gcaaaaagct gtcgacgaaa ctcctcataa acaggtagta
    23341 aaatacgcca tgtagttata aattcttcct tggtttttcc ctcagttgaa ggcaggaagt
    23401 gctgccagaa ctcctgaatt gctaactttt gatttggggt aaactgatca aacaccgtat
    23461 caagctcttt cagttccttc acattggtga aaagctgacg tgcatcaatc tgatatttat
    23521 ccacttcatt gaaatcggca agcaaaatct cgccccagta acagaaagta tcgaatgttt
    23581 cctcgctgcc gctcagttgt ctgtaaatac tgaacaaccg gaaaagttcc cttgtacggt
    23641 cagccacttt gcgttgcgat gcgctgaaaa aacattcgtt tatggtgaga attccgggcg
    23701 aaaaaaccgg tttatcaatc agttcggata aatacttctg aaaaaaaagt cctgctcttc
    23761 gatttgggaa cacaaaagtg aagtgatata tatcttgctt atattcatca caatacgctt
    23821 tggcaacacg atgcaaaaaa gatttcatat cttacgctat tttttcagaa gtgatagcac
    23881 tgggtagtta gtttatattg tcaaattcat tcagctaaac aattccttac caaaatgaaa
    23941 caattatatt tatcaaaatg atcagaacca atggaagtgc taaattacat aattttattc
    24001 aatcaggcag attaactggt ttttttgtta cttttgtcaa taaaaaaaaa cagaaaacaa
    24061 gattataaat gattggaacc atagtaaata cggcaagtgt tatagcaggc ggatgcatcg
    24121 gtttaatgct caaaaaaggt atgcctgaaa gaataaaaac catttatttt caggctgtcg
    24181 ggatatttac gcttgccatc ggtatcagca tggtttggaa tatggaacac attctcgtca
    24241 ttgtgtcgag tctggtgact ggtgccatac tgggcgaatg gtggaatatt gaaaaaagca
    24301 ccgaacaact gagtgaatgg acgaagaaga aactccacat tggtagcgaa aaattttcag
    24361 aagggctgac tacttccttt ttgctgttct gcatcggggc aatgaccatc atgggagcca
    24421 tcgaagaagg taccggaggt acttcgcggc tacttttcac aaaatcggtg atggatggtt
    24481 tttctgctgt aattcttgct tcagcattcg gagctggtgt ggttttttct gctgtaccgc
    24541 tgttgatttt tcagggcgga ctgacattgc ttgcgcgcta tgccagtgac ttgttgacgc
    24601 cggaaatgat taaagcgctg acaagtttag gtggtatact gctgattggt ctgggaatta
    24661 acattctgga gataaagaaa ctgcgcatca tgaacatgct tccggcattg gtggttgtgg
    24721 tgattttcat gtggattatt gttaaattca acatacggtt actatgaatg tgaaatttgc
    24781 acttatctgg tttttggtgg gctttgtgct tgttaatgct ctgaaaatac tacataccat
    24841 tctcagcagc agaaaaaaat tcaacagaaa cagtcagaag gatattccgg tctcagctta
    24901 ctacaaacag gcatatttca acaccctgaa gtttcagcct ttttattacg tgattgtctg
    24961 gcttacatgc agttacattt atttcaccac acacatctca aataatctgt ttactgatgc
    25021 actcgtcaca gggataagtt ggtgggcgct gaccgcaata gcagaaatgc tgatctgggt
    25081 actggtcaac cacaagatga atttaacctg gaaagaaatg tatcttcaat cgcagccgtg
    25141 gatatcgctg acttattact cggtgcttgt cagtccgctt gtgctgtcgc ttatattgca
    25201 ttaaatcatt gctttgtgag aacaggcaac gcctctccca ctacaaaatt gctaccgccg
    25261 atatatacga aatcctgtcc ggaagcatca ttcagtgcag ccctgacagc cttctgaaca
    25321 tcagcaaacg catcacctct taatccttgt ttttctgcct gttctttcag attttcggca
    25381 ggtaaagctc tttcggtttt tgcatttgta aaataataga ctgccttcac aggaagcaac
    25441 ctcaacatac cggtaatatc tttgtccttg accataccga tgacaatccg cattttttca
    25501 cattgctgtc gtttgagttg ttgtgtgatg tattcaaaac cacctttatt gtgtccggta
    25561 tcaagtacaa ccacagggtt ctgttggaca atctgccatc tgccctgcag gttggtcagt
    25621 gcaacgactt ccttcagtcc tgtgcggata tcttcttcat atattgcaaa acccaatttc
    25681 tgcaattcct ctgtggcggt gatgacggta gcaatatttt tcaactgata aataccacca
    25741 agtccgatgc gaagttcatt aaaacctgga gcattgacaa tcatgacatt ttgctcataa
    25801 tcatcatatt ccaccttata tttatcttca gcaaaaatca gcggagcctg aattatctct
    25861 gctttttcga tgaaaacgga tttagtttcc ggcaggtatt cgccgatgac aaccggcgta
    25921 tgctgtttta taattcctgc tttttcagct gctatctgag ccagggtatc gcccagaaac
    25981 tccgtgtgat cgaaactgat attggtaatg accgaaagtt cgggttttac gatattggta
    26041 ctgtcaagcc ggccaccaag ccccacttct accactgcca catcgacctt gcagtcagca
    26101 aaataacgaa atgccagcgc catggttgct tcgaaaaatg agggttgtat ttcgtcaaaa
    26161 actttctgat tattctcgac aaaaccagct acataatcct gattaatttt ttctccatcc
    26221 actcttattc tttcgccgaa atctaccaga tgcggcgaag tgaacagacc ggttttataa
    26281 cctgctgatt gcagaatggc agataaaaaa tgtgatactg accccttacc attggtcccg
    26341 gctacatgta ttactttata ttcatgttgg ggattgccta actgttccat caaacggatc
    26401 gaattttcca gtcccggctt gtatgcagca gcaccgatat gatgaaagac tggcaagcgg
    26461 ttatacaaat actctattga ttcctgatag gtcatttatt aatatttatt ttcggaaatt
    26521 acctgagatt tctctttacg gattttctga cgtgtttttt gggccgaata acccacgaca
    26581 atgattagaa ccggacgttt ttctttggga atttccagta cttttctgag cttcttttca
    26641 tcaaaccagc ccacgatgca ggagccaagt ccttcatcgg cagcagccag acaaagatga
    26701 gctgccagaa tgcccagatc gaagtgcggg aaatgcttac tttttgccca gctgcctgct
    26761 ttggagctaa gattggacga ctcctccacg attacaatct gtacaggagc ctgacgggta
    26821 aaatgattgg agctgacaat aatatctgag gttgtggcag ccacttcatt acgtattttt
    26881 tcatccctga tcacaatcat cttccacggc tggccgttgc aggcagatgg agcaagcctt
    26941 ccggcttcga gaattctgtc gagcttatcg gtttcaatat cgcgttggag ataagcacga
    27001 tcactctgac gctgcatcac caattcaata aaagtattca tgttgttaat gttttttgta
    27061 ttaaaaaaaa cagaaatttt cgacccgcac cgctataaat atatactttt gtaaccggta
    27121 acaaaggtac acaattggat ggaaacgctc aaaaaagttc agctatttat cgaaaacaac
    27181 cagcttctca cccctaaggg tcgtgtcatt gtcggtgtaa gcggaggtgt cgattcggtg
    27241 gttatgctca atatactgac cgtttcgggt tatgagtgtg tggtggcgca ttgcaatttt
    27301 catcttcgcg ggaaagagtc ggaccgcgac gaacagttcg tggctgactt agcccaaaaa
    27361 tacggattga tctgtaaaaa agtggacttc gatactgtca actacgccaa acgacataaa
    27421 atctccattg agatggccgc ccgagacctc cggtatgatt ggtttgccgg tctgctgaag
    27481 gaaatgagag ctgaagcaat agctgtagcc catcactctg acgattcgat agagaccatg
    27541 ctgattaact cgataagagg atcgggattg cgaggattgt atggaataga tgaacgcaat
    27601 ggaaacatta tccggccaat gcttagctgt tcgcgcgaag aaattgaaaa ttatgcccat
    27661 aaaaacaacc tcaactatgt ggttgactct acaaatgctg ccagcgacta cttgcgtaac
    27721 aagttcagaa atgagattct gcctgctctg gctgtaataa atccttcggt aaaggtaact
    27781 tttctggaaa acatgaaccg ggtgcgcgga gcctggaaaa tctatgatca gaaaattcgg
    27841 gaaataaaag aagaaatcag ctttacaaaa gacaatcagt tgtatatcga cctggaaaaa
    27901 ctgaaacaac aggtcgatgt tacgacagta ttatttgaac tccttcagcc ctaccacttt
    27961 aaaaacagta ttgtaaatga catcgtcgac gggctgaaaa acaattcagg agcaaaattc
    28021 ttttccgata ctcactgtgt actcaaagac cgaattcacc tgattgtcag aacaaataaa
    28081 accaaaagcg atatcgaata cagtattgat aaggaaaccg ccagcataac cgaacctgtt
    28141 tcaatggagt tcagagaatt tctgaaaact gatgattttc agttctcgcg caagtctgat
    28201 atcgtgcatg ttgatgccga caaactgaaa tatcccctta tattacgtcc ctggcgaaaa
    28261 ggtgacacat tttatccctt cggtatgacc agcaaaaaaa aggtgagcaa cttttttatc
    28321 gatcagaaaa taaaccggtt tcagaaggac gatatctggt tactcatatc ggacgaagaa
    28381 attatctgga ttgccggttt acgcctcgac aatcgtttca ggattacaga agagacccgg
    28441 aatatccttg aaatacgcat caatcaataa ttttttctta cagatttgct gattacaaaa
    28501 aaatacagta attttgcaaa gttttcaaat aaatgtctat tgtcgtccga cgaagtcata
    28561 acatcaacaa tttttttcac attataacaa atctcgtttt tccccaaaaa caaacaggga
    28621 aatctcccca atacgtatta tttattcaca tatgacgaat tacaatattc tgcaattaaa
    28681 cgcgaaagaa ctttcggaac tgaaagaaat cgcagttgaa ttaggactga aagtgtcaaa
    28741 ttcagcaaca aaagaaaaac tggtttatga aattctggac caacaagctg ttgcaaatgc
    28801 acagcaaaaa gcaacagcta atgatggaca agatgccaac cgaagaaaaa gaatgaggat
    28861 cattcagaaa aagggaaaag atgaaaaagt ttacagtaca caaccagctg tcgcagaagc
    28921 aaaacctcag acccctgcac agcaggtaaa taaaactaaa caagaaaaac cagccactcc
    28981 atcaactccc gaatccccta aaactgaaga aaccgttcag aaacagggac aaaagaaaaa
    29041 aacttccgca aagagtaaaa aagcacaaaa tatacagcca gttacgaccg atacgccacc
    29101 tgcaactgct gcaccagaaa aagaaaacat ccctgtacaa ccagttcaaa acgagcaaaa
    29161 agtgaaaccg gtgaagaaag tggtcctgct tcccgaagtt gttgatgaag tcgtagagcc
    29221 ggaattgcca gtagaaccgg aacaaaaacc ggaacaggaa gtgaaacctg ttcagacgac
    29281 tgaaactgaa ccgattgcag tgatagcaga aattaaaaca gaacagatac ccaatcaaaa
    29341 acctaccaaa agcgctcaga caaatcaggt cagcggacaa ccgaacggca accgcgttaa
    29401 atttaatccg cgtcaggaaa aggaaaaaaa ctacgatttc gaagaagtac ttgaaggaac
    29461 cggaacactc gaaatacttg cagatggtta tggttttctc cgctcatccg attacaacta
    29521 cctttcatca cctgatgata tttatgtttc acagtcgcag ataaaacttt tcggactgaa
    29581 aacgggcgat accgtgaaag gctccattcg tcctccgaaa gaagttgaaa aatatttccc
    29641 gctggtaaaa gtatctcaga tcaacggccg ctcacccgag tatgttcgcg acagagtgcc
    29701 ctttgaacac cttactccgc ttttcccgga cgaaaaattc aaactcaaca ccggaaaaaa
    29761 cgacacactt tctacacgca tggtcgatct tttctgccct atcggaaaag gtcagcgcgg
    29821 actgatcgta gctcagccca aaaccggtaa aaccgtttta ttaaaagaga tagctaacgc
    29881 gatttcagcc aatcaccccg aagtgtatat gatcattctt ctgattgacg aacgtcctga
    29941 ggaagtcacc gacatggcac gcagtgtaaa tgccgaagtg atttcttcga catttgatga
    30001 accggcagaa cgccacgtaa aagtagccag catggtgcat gaaaaagcca aacgaatggt
    30061 agaatgcgga caggatgtgg tcattctgct ggatagcatt acccgtctgg cacgtgctta
    30121 caacacggtt tcacccgctt cgggcaaagt actgtcaggt ggtgtggatg ctaatgcgct
    30181 tcataaacca aaacgtttct tcggagcagc ccgcaacatc gaaaacggcg gaagcctcac
    30241 catcatagcc acggctctga ccgaaaccgg ttcgaaaatg gatgaagtaa tttttgaaga
    30301 attcaaggga accggcaata tggaactgca actcgaccgg aaactttcga ataaacgtat
    30361 cttccctgca gtagatatta tggcatcgag tacacgtcgc gaagacctcc tgcttgatca
    30421 ggatacgctg aaccggatgt ggattcttcg ccgccacctg gctgacatga attccattga
    30481 agctatggag ttcctgaaag acaggctgga acgcaccgaa aataatgaag aatttctgat
    30541 atcaatgaac ggataattcg tttccggaag tctgaaaaat atgaattcag caaatataac
    30601 cataaagccc gttaacagcg ggctttatta ttttcaggaa ggctaactgg tcaggcttcg
    30661 agccactctt cgataaatat atgattactg gctacaaatt tcggctttga aatgcggctt
    30721 tcaccagcag gagcaatggg aaacctgcta aaattccaat cgttgggttt gtcaaagtac
    30781 tgataggcaa gccagttggc cgaccactcg gtccaggtgt gcatatgcgg tatatttttt
    30841 cttctttgat tgaaagcact tttaaggctg gccgagacta tgtaatgcca gaaaaaccag
    30901 aaaccccatt tgcggaactg aagaatatgg ccaaactcgt gacgtagaag ttccgtatca
    30961 tgttgcattc ccttcccgac aaaaatacca attccgggaa ttgtcatggc agctcccttt
    31021 ttgaaattgc caacagaaag cttaaatatt cttacaccgt gaaacttcat ttcttctttc
    31081 ttttcattac tctttcgtac acttcaatcc atccttctac actcactttg cgggcaagat
    31141 ctgtaataag ttcatagggt atatcttcag gtttctttaa ccggatacaa ccgcgtccca
    31201 tatccagttt ggatttactg tgtttcgggt actcggtctg aaaccattcg agaagctccg
    31261 gcatagcaga aatgctcatg tgatacaacg caatgtaatt tttctgagaa gccagattga
    31321 taaagggtaa tggtaatttg ggatttgact gatacccatc cggatacagt gtatgcggaa
    31381 ccacatatcc aatcatgcca taactgattt cttcgtgata gcctttagga agattttcac
    31441 gtaaaatact tcttaatttc tgaataattt cccgccggtc atcgggaagt tgagctacat
    31501 actcatcggg agtggtaaat gcaatattca taatgatttg attttttaca aatataagcc
    31561 gaaataatca tatacaaaac aactcacgcc tttcaatact taagaaatta gtcagcagga
    31621 acttagagaa tatcaaaata aattcttaat tttgaaaaat aaatcagaac ttcagaaaac
    31681 caaactacaa actatcagaa tggtgtttta ttgttcttat tccggtgtat ttcaatgatt
    31741 ttttgtaaaa tccaggatcg ggtatccatg ccgtaagccg gaccgatgac aaatattttc
    31801 tgtaaaaagt tacacacaat gatgaaaaaa atactttcta tattaattct ggccttgtta
    31861 tcaagtcacg tctttgcaca ggatattctc gaatatgaaa ctcgaacttt cagtccttac
    31921 tggtacacag gtataaacgt gggttcgaac ttatatatgg ccgaagggaa tgatttcttg
    31981 tcgggaaatg atggcgtccg tttttcgtta agagataact caggaacgac catccgcgcc
    32041 actttcggaa gaagatttac acctgtaatc ggtgtaagag ggtacgcagg ttacagcagt
    32101 tataaatggc tcagtgctta tcatcacaat ccggtcgtta cgtttggttc ggagtttatt
    32161 acggcagacc tcaccattag tctgataaat atgctggaag agaactactc actgaaaaga
    32221 tatgatctgg gattgttttt aggaaccgga ttacaattgc gtaatgagat aacctcaccg
    32281 gctgtagcag cttcaaacag cagcctcaca cctgtcgggc ggctgggact ggaagccagt
    32341 tttttgctga caaactatct gggtttaagt tttggagggg aacttaattt tgtcaatgac
    32401 aacaacaatg gtatggttgc aggacgaaac tatgaattca ttcccgcatt ttccgtcgga
    32461 ctgaattatc agctaaaagc gggcaagaaa taattttcct gaaaaagaat aaaaaaacag
    32521 gtctgaaaat ttccggagaa gggatatttt cagacctgtt ttacgttttt aaaagatgtt
    32581 tgaacgacag attttatccg tagatttcac ctttcgcagc cagcagtgta tttttcagca
    32641 gcgaaacaat cgtcatcagc cctactccgc ccggcacagg agtaatgtat gaacatttcg
    32701 gggcaacttc atcgaatttc acatcgcctt tcaagcgaaa accgcttttt gtttccgtcg
    32761 aaggtacccg tgtcgttcct acatcaatga ttacaactcc gtctttcacc atgtcggcag
    32821 tcaaaaactc gggctgtccc agcgcggcaa tgatgatatc agcctgcaag gttatttctt
    32881 tcagattgcc ggtgcgacta tgacaaacag taactgtcgc atcacccgga acggatttct
    32941 gcatcatcag cgacgcaatg ggtttgccca cgatattgct gcggccaatc accacgcaat
    33001 gttttcctga agtcggtatt tcgtagcgtt tcagcagttc gatgatgccc gatggagttg
    33061 ccgaaacgta gcatggcatt ccgattgaca tgcggccgat atttatcggg tgaaaaccgt
    33121 caacatcctt gcggtagtcg atggcttcaa ttactttttg ttccgaaata tgtttgggca
    33181 acggaagctg aacgataaac ccgtcaacat cttcatcctt attcaattct tcaattttcg
    33241 acagcagttc cgcttcgctt acagtatctt cgtattcaat tttggtcgat ttaaaaccaa
    33301 cctgttcgca agatctcacc ttacttgcca cgtaagtttc gctgcctccg tcgtgtccga
    33361 caattattac agccagatgt ggtcttttct taccggcagc aatcatttgc tttacttctc
    33421 cggcaatttc cgccttcacc tgttcggcga ttacttttcc atcaattaat atcatattta
    33481 cctcctttta atctccctcc caaagtggga atttcattgt taattcatca aaaaagcccc
    33541 aaaaaatccg attgaaggat taataagggg ctaaattgtt atcgtctttt taatttttgc
    33601 ttgaactgtt gttgcgatgc cattttcatc atcttactca tctggtcaaa ctgcttgagc
    33661 agccggttga cttcgatgat tgtggtcccg ctgccggctg caatcctgct tttccggctg
    33721 ccattcagca ctgtaggagt ttttcgctcc agcggtgtca tcgaatggat gatggcttca
    33781 atacctttga acgcgttatc gtcaatatca atatccttca actgtttgcc catgccgggt
    33841 atcatagaag ccaggtcttt gatattaccc atttttttta tctgctgtat ttgggacata
    33901 aagtcgtcaa agtcaaactg atttttagca attttcttgc tcaggcggcg cgcttcttcc
    33961 tcatcgtatt gttcctgtgc tttttcaacc agcgaaacga tatcacccat acccagtatt
    34021 cggtcggcca tacgttcggg atagaaaatg tccagcgcat ccattttttc gccgatacct
    34081 acgaatttga tgggtttatc gaccaccgag cggatggaca gggctgcacc accgcgcgta
    34141 tcaccatcga gcttggtaag tattactccg tcgaaattca gccggtcgtt gaattctttg
    34201 gctgtattta cagcatcctg acccgtcatt gagtccacca caaataaaat ttcctgcgga
    34261 ttaatggctt ttttgattgc tgtaatttca ttcatcatct gttcatccac tgccagacga
    34321 cctgctgtat cgacaattac cacatcatga ccgtactgtt tggcatgttt gatagcagct
    34381 tcggcaatgg caacagggtt tttactggtt tcgtcgaagt aaacaggcac attgagctgt
    34441 gctccgagca ctttcagctg ttcgatggca gccggacgat acacgtcgcc ggcaaccaac
    34501 aaaggatttt taccccgttt tgttttcagc aggtttgcca gttttcccga aaaagtagtt
    34561 ttacccgaac cctgaagacc tgacattaaa atgacagcgg gagaaccttt cagattgata
    34621 tccacactac tgccacccat gagttgcgtc agttcatcgt gcacaatctt caccatcagt
    34681 tgttgcggtt tcaccgatgt gagcacattc tgccccatgg ctttcgcttt caccgattcg
    34741 gtaaatgttt tggctgtttt gaaattgacg tcggcatcca gcaatgcttt gcgcacttcc
    34801 ttgagggttt ccgctacatt tatttcggta atttttcctt ctcctttgag gattttcagc
    34861 gaccgttcta acctttcact taatgactca aacatatatt tttttgatat ttaatgtgtt
    34921 ataactctgc gtcagtcatc tgacctgtat atttttaagt ttgcaaagtt aacaaaaaat
    34981 gacttaaccc acaatatgat aagatcttaa taaggagaaa taaaattaat attttgtagg
    35041 tcttcgtatt tacagtattc atataaccaa ctttataacc gaattcttct aaaatcccaa
    35101 aatatttaac aaattcaggc ttagctttta aaaaaaacct atatttgcag ttgataactt
    35161 tgaaagttca caaaatccat gaaaaagctt ttattcctct cacttttctg cattattttc
    35221 tgttccgcag gtaaagcaca aaccgcgtca atacaaaaca tcatatcgga aatacaaaaa
    35281 aaactggtac ctgataagcg cgtctgcatc tttgaggtaa atcttattga agacaacagc
    35341 gaaagcgtgc tgaaaggaga aatttcggat gccggtattc atcagacact gatatcggaa
    35401 atcagacagg ttcaacccga agtaaaagac agtattcgtg ttctgcccga ttcatcattc
    35461 ggtgaaaact attggggaat aatacctcta tccgcgatat ttatccggca gaaaccaaat
    35521 ttcggagctg aactcaccac gcaagcgcta atgggcacac cagtgaagat attacaaaaa
    35581 aaaggaggat ggctgcaggt acagacaccc gacaggtaca tcgggtggac cagtaccgac
    35641 aatattgaac ggataaatca gacacaactg aatgagtata accggttccc caaagccatc
    35701 gtcatttcac atcaaacacg ggttctgaaa aatccccaaa aaaaatccga ccagattaca
    35761 gaagcaatca tgggggatat ggtggtgatt actgaatcga aatcagctag gtcaaaatat
    35821 tgcagcgtaa cgctaccgga tggaaaaacg ggttatgtaa agaaagaaga tatccgctta
    35881 atgcaacacc gggaagttaa tctgagtgcc aacagcttaa tcaaactaag taaaactttc
    35941 atgggattgc cctatttctg gggtggcacc accagtcgcg ggatggattg cagcggattc
    36001 accaaaacgg tgttttttat gcacggaatt attctaccgc gggatgcttc acagcaatac
    36061 tattgtggca ctgaagtcga tacgagtgcc ggatggggaa atctcc
//
LOCUS       DAOPYB010000014        33523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1220_length_33523_cov_10.612227, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000014 DAOPYB010000000
VERSION     DAOPYB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 33523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33523
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_33523_cov_10.612227"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="PKH58_02180"
     CDS             complement(<1..1553)
                     /locus_tag="PKH58_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX87865.1"
                     /translation="MNKDNISQSSELPLQGVGGLIYISQFYGKDSRFVIAGGGNTSYK
                     NQEKIWVKASGSSLATITEEGFAVLDREKLNLMSEKVYSADTSEREEQVKNDLAYATI
                     TKGKRPSVETSMHNVINYAFVVHLHPTAVNGLMCSQNAEADLIKLFGEKTLYVEYTDP
                     GYVLFKKVDDKIKAFRAKFNEEPKVIWLQNHGIFVAADTIDEIKCIYQEIFNTLDKEA
                     PQPPKGGVSIPPSGGKGAEILPAIRMMLSDDGLKTLKVRNNELVKYFSESSENQRNIA
                     RPFTPDAIVYCKSNYIFAPQPPKGGELTPEEIISYFEKEIKNFRVKFGYQPKVLLIKG
                     IGLVAAGDNAAQCEIILDVFEDAMKIATLSQSFGGAHPMTQAQIDFIDNWEVENYRRS
                     VAAGGVTGRAENKTIIVTGAAQGFGEGIARNLLQQGANIVVADLNEEVGNATVAHFNE
                     IAKSNRAIFVKTNVSEIQSIENLIQQTICNFGAVDCFISNAGVLRAGSLEDMTPENFD
                     LVTKINYNA"
     gene            complement(1620..2777)
                     /locus_tag="PKH58_02185"
     CDS             complement(1620..2777)
                     /locus_tag="PKH58_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNX87866.1"
                     /translation="MSYMKVNAHLHTPYSFSAFSDISDALDRAVAEGVKVVGINDFYT
                     TAGYEEWSVGCKNRALFPLFGIEFISLNEEDQKAGLRVNDPGNPGRTYLSGKGMSYPF
                     SLVEPYTSLLADVRKEANNQVKAMCGKLNELLKTNNAGFSLDFEWIKNELTKGSVRER
                     HLAKALRMKVYEIYEDNEEEIKSLFTKLFGGKELKSEINDVAGVENEIRANLLKAGGA
                     AFVPEDAKAFLPMQTVCEIILTGGGIPTYPFLADDAKGGYTDFENDLQRVAKQLTERG
                     FHSVEFITTRNDVSLLEKYAEYLFDQGFIVTFGSEHNTPVMEPVELFARNNTPLTEKL
                     MQINFEGACVVAAHQHLVAQGFKGYVDEKGNADRSNRAEFVKLGKELITAP"
     gene            complement(2794..4164)
                     /locus_tag="PKH58_02190"
     CDS             complement(2794..4164)
                     /locus_tag="PKH58_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HNX87867.1"
                     /translation="MKNTGGLWLMLMLLLQLLVTQQVIGEVPTTGIKVKAPFQMPVIR
                     VPDFSKCKTYTITDFGAVQGDKSKITQAIARAIEVANNSGGGTVVVPEGEWITGKIHL
                     KSNVNLHLMKGAVLLFSDDPGEYLPAVHSTWEGLECYNYSPLIYAYECENVAITGEGQ
                     VKALMDNWKKWAGRPRPHMNSLKNLYYWAFDNKPVNERIMVNDSSNLRPQFIQMNRCQ
                     NVLIEGVNIINSPFWTIHLYLSKDIVIRNVSVYAHGHNNDGVDPEMSQNVLIENCTFD
                     QGDDAIAVKSGRSPEGWRTRTPSKNIVIRNCTVKNGHQLLAIGSELSGGIENICLKNC
                     TVSKGAKMFHLVFIKTNERMGGYVRNIYVENIKAGSVQNGVLGIETDVLYQWRDLVPT
                     IEKRLTPIKNISLKNMKADKVEFISRILGQKELPVENVKLKNIAADTISSKKHIHENV
                     LRFSEK"
     gene            complement(4260..6719)
                     /locus_tag="PKH58_02195"
     CDS             complement(4260..6719)
                     /locus_tag="PKH58_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX87868.1"
                     /translation="MPKIQFIDPSQARQPGFVEFQPIPVNQYQKTVKDEKGNFSDEEF
                     KAIYHDMALIREFEIMLNLIKTKGEYNGTPYNHPGPAHLSIGQESAAVGMAWTLTVDD
                     FIFGSHRSHGEILAKGMSAIHKTDDDELLKIMESFFDGAVLNVVKKDFNGTTKELARR
                     FLVYGTLSEIFARETGFNRGLGGSMHAFFTPFGVYPNNAIVGGSGDIAVGAALYKKVN
                     RKPGLVVANIGDASMACGPVWEGITFAAMDQFNNLWEGDMKGGLPVIINIMNNQYGMG
                     GQTCGETMGYGIAARIGAGVNPEQMHAERVDGYNPLAVIDAYKRKRKIIEDKKGPVLL
                     DVLTYRYSGHSPSDASSYRTKEEVEAWENQDCIASFGKQMIEAEVTDQHELDAIQADV
                     KELVYEMFLKAIDDEVSPRMKNPEVISDMMFSNGSVESFSDAKPDVLMPLEENSRVKK
                     IAAKERFAFDADGKPFSKMKQYQLRDGIFEAIMDRFYKDASLIAYGEENRDWGGAFAV
                     YGGMTEALPYHRLFNSPISEASIIGTAIGYAMCGGRVIPEIMYCDFLGRCGDEVFNQL
                     PKWQAMSGNVLKMPVVVRVSVGSKYGAQHSQDWTSLVAQIPGLKVCFPVTPYDAKGLM
                     NAALQGTDPVIFFESQRIYEIGEQFHKGGVPSGYFEIPFGEPDVKKEGNDITFLTVGH
                     TLYPALQAAKVLEEKYGMTAEVIDARSLVPFNYEKVIASVKKTGKIIVASDATTRGSF
                     LNDLAANIGQLAFDYLDAPVCVLGSRNWITPAYELESSFFAQPTWFLDVIHERIQPLK
                     DYIPGQNFTSAELIRRSKSGV"
     gene            complement(6803..8158)
                     /gene="lpdA"
                     /locus_tag="PKH58_02200"
     CDS             complement(6803..8158)
                     /gene="lpdA"
                     /locus_tag="PKH58_02200"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNX87869.1"
                     /translation="MYDLMIIGGGPAGYVAAERAGHKGLNVILFEKKSMGGVCLNEGC
                     IPTKTLLYSAKTYENALHGDKYGIFAENVRFDFGKIVARKNKVVRKLVAGVKSKMKEN
                     NVTVIEGEAIIQGRSEKGVEVNCNGESYLAKNLLICTGSEASVPPIPGLAEAGDVILT
                     NREILELKEQPATLVVIGGGVIGMEFASFYNSLGTKVTIIEMLPEILGGLDFEVSAIL
                     RDIYAKKGIEFHLNAKVVRVEGNTVVFEKDGKTETIQGEKILLSVGRRAVVKGYGLEN
                     LNVEMTRGGIKVDEKMHTNVPGVFAAGDVTGFSLLAHTASREGEVVVNNLTGRNDVMR
                     YNAIPGVVYTNPEVAGVGETEESAKAKGIPYKVIKLAMSYAGRFVAENEGGNGLCKVL
                     VGEKHGEVIGVHMLGNPCSEIIYGACMAIEQEMTVKELQEVVFPHPTVSEIFKETIFS
                     I"
     gene            complement(8382..9704)
                     /locus_tag="PKH58_02205"
     CDS             complement(8382..9704)
                     /locus_tag="PKH58_02205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNX87870.1"
                     /translation="MAIIIVMPKQGQSVESCIISEIKKKGDVVKKGDILFSYETDKAS
                     FEEESTADGVVLECFCNEGDEVPVLDNVMVIGQPGEEVPLDLLTDTKPHAEADVKKEE
                     TAKTEIKIQEEVIISQLKPTGGLSPRARNLAEKEALDISQISGSGPDGRIIEKDVQSV
                     LENRPKLTSLAKKIVIQEGMQPQGWGNGLAGTITSHDLSVPVNAGSVADYEDKKISNM
                     RKIIAKSMHASLQNSAQLTHHLGADARRILELRKQVKTAMEAGKLETNITLNDMVCFA
                     VIKALKKFPNVNTHFLGDTVRYFNKVHLALAVDTERGLMVPVVRNADDLTIGGLAQQF
                     KEMANACRKGSINPELLSAEAATFTVSNLGNYGVEMFTPVVNLPQSAILGVNTIVPRP
                     KDLGDGVYAFVPYIGLSLTYDHRSLDGGEATRFLKQIAIEIETLELVL"
     gene            10042..11130
                     /locus_tag="PKH58_02210"
     CDS             10042..11130
                     /locus_tag="PKH58_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87871.1"
                     /translation="MIAKFQLTLLFSVILIFYSNAQNTISTPQATNRENNSKLLRQTT
                     GNEPDNSIKPNLNINSDFLHPQSRLIFPSRLNDFQRNRIIFTQNNPSKIDVIYYSADS
                     GNEIAISLYRYETGTEGRLRNEYLNAIRKLTKEENVDSIPRAIPVKYKGLKYICNGVK
                     GRYDFSETAHTELCVYECGTWMMNIKIRSKNLNSNELIELEKILTNTFDPSWLTSQNP
                     LLLKSNVDFERSALADTVMTAAMVASAFKKLDWATYNVRANERASGFPDLYLNMHIAS
                     IREFIQIQQKKPRLYVSPSAEKYFKELSEINNAGFLPEFIMEQYQNIMFVPDGQVLNH
                     EGYKKWKENKNLTIDLNRKMYTICYRKQ"
     gene            complement(11158..12996)
                     /locus_tag="PKH58_02215"
     CDS             complement(11158..12996)
                     /locus_tag="PKH58_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX87872.1"
                     /translation="MFDFLTLIKRFAPPYKKYIFLSLVFNLISTIFSLFSFAAIIPVL
                     QILFGLSKADAVYVSWNWDDGLSAVFAAIKNNLGYYVQQLIESAGPGMALLYLGLFLI
                     IMTFFKTGTTYLASYFVIPIRTGVLRDLRNKMYKKIISLPIGFFSDERKGDIMSRMTG
                     DVVEVEASIISSLDMVLKNPLMILIYLIVLFAMSWELTLFVLVLLPVSAWLIGVIGRS
                     LKKRSTLGQEQTGELLSQIEETLGGLRVVKAFNAEEKLTQRFGILNEKIRHTFNRIHR
                     KYNLAHPVSELFGTIVIAILIWFGGSLILQQNSSIGAAEFIYYLVIFYSIVPPAKELS
                     KASYSIQKGMASLNRIDKILMQENNIPDPEVPAMLPAIIEKIEFKDLSFRYQSEWVLK
                     HINLTIPLGKTVAIVGQSGSGKSTLVDLLPRFYDPAEGGIFFNNVNIKDYRKSDIRSL
                     MGNVNQEAILFNDTFYNNIAFGVELATREQVINAAKIANAHDFIVATENGYETCIGDR
                     GSKLSGGQRQRISIARAILKNPPILILDEATSALDTESEYLVQEALDNLMKNRTTVVV
                     AHRLSTIKRADLICVLHEGKIVERGRHEELMKQGGYYKKLVDMQSF"
     gene            complement(13042..14385)
                     /gene="purB"
                     /locus_tag="PKH58_02220"
     CDS             complement(13042..14385)
                     /gene="purB"
                     /locus_tag="PKH58_02220"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNX87873.1"
                     /translation="MTINSLTAISPVDGRYSSKTEAYAAYFSEFALIRFRVLTEVEYF
                     IALCELPLKQLESVPVNILPKLREIYENFSIGDAQKIKDIEKVTNHDVKAVEYFLKEK
                     FNEIGLGAYREFIHFGLTSQDINNTSVPLSIKKGMEEVYYPELDNLILKLRRMADEWR
                     DVSMLAKTHGQPASPTRLGKEIMVFVSRLNKQLELLKAVPQSVKFGGATGNFNAHHVA
                     YPNIDWKLFANKFALEKLGLEREQWTTQISNYDNLAALFDALARINTILIDFCRDIWT
                     YVSMEYFKQKIKAGEVGSSAMPHKVNPIDFENAEGNLGVANAMFTFLAQKLPVSRLQR
                     DLTDSTVLRNVGVPFSHTLIATQSILKGLDKLLLNETALYRDLDNTWAVVAEGIQTIL
                     RREEYPSPYEALKALTRTNEAINEKAIREFIDKLDVSDSVKEELRRVTPRNYTGI"
     gene            14573..15244
                     /locus_tag="PKH58_02225"
     CDS             14573..15244
                     /locus_tag="PKH58_02225"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNX87874.1"
                     /translation="MKYIKIHKEGKMILAFTLVVLLVINVVVYMQFPKPFLAINITLS
                     AFLLIFVMFFFRNPRRVVEIDDPSLIIAPADGTVVVIEPTDEFEYFGDKRIQVSIFMS
                     LFNVHANWYPVAGKVIKTAHHSGRHMAAYLPKSSTENERSTIVIETPSKTQILVRQIA
                     GALARRIVTYAHVGKTCHLNEHIGFIKFGSRVDMFLPLDAEIFVEIGESTTGNETIIA
                     RLNED"
     gene            15247..15576
                     /locus_tag="PKH58_02230"
     CDS             15247..15576
                     /locus_tag="PKH58_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNX87875.1"
                     /translation="MTLQEAQTRVDEWIKTYGVRYFSELTNLGILTEEVGELARIMVR
                     TYGEQSFKETDKNNSLADEIADVLWVLICLANQTGVNLTDAFEKNLLKKTNRDNNRHF
                     ENEKLKH"
     gene            15590..16471
                     /gene="deoC"
                     /locus_tag="PKH58_02235"
     CDS             15590..16471
                     /gene="deoC"
                     /locus_tag="PKH58_02235"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNX87876.1"
                     /translation="MNKFEELFNAYNCDTLTDETVKKHVEQILTGHFDENNNIGVYKQ
                     CFNQIDLTSLRETDTEAEITAMVEKVNNFDNNFPDIPNVAAICIYPALVPVVKASLTE
                     PIGIAAVSAGFPASQTFVEIKVAETSMCVMEGATEIDVVISVGKFLEGKYDEVYEELT
                     EIKSACRDAHLKVILETGALKTASNIKKAAVLAMQAGADFIKTSTGKIAVAATPEATW
                     VMCQAIREWNEKTGIKVGYKPAGGIANTDDAVKHYTLVKEILGNEWLDNKLFRFGASS
                     LANKLLTSINKEEIKYF"
     gene            16599..17144
                     /locus_tag="PKH58_02240"
     CDS             16599..17144
                     /locus_tag="PKH58_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNX87877.1"
                     /translation="MNTFDLFIFIPITLGLIFGLFRGFVKEVVSLVAVILAIVAAEIF
                     SSLFSDFLAGLFNVSNKVSATMAYVFVFIITIVIALLIARMAEKLISNISLGWLNSLL
                     GGVFGALKFAFIISVLMNVFDAIDTKFHLAKPETKIESVGYYPVLKLAPSLWKHSKEA
                     YQKSKTSENEGQSTSPENEAN"
     gene            17131..17733
                     /locus_tag="PKH58_02245"
     CDS             17131..17733
                     /locus_tag="PKH58_02245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNX87878.1"
                     /translation="MKQTNLPSNYSGQFPECGCDEAGRGCLAGPVVAAAVILPPDFEC
                     AELNDSKQLTEIQRDALRIIIEKEAVSWSVAMVSEKEIDEINILNASILAMHRAIDKL
                     AIKPCFIIVDGNKFKKYQEIPHQTIVKGDSKYLSIAAASVLAKTHRDELMMKLHDEYP
                     QYAWNKNKAYPTIKHREAIASYGVTPYHRLTYKLLKDNNL"
     gene            complement(17728..19275)
                     /locus_tag="PKH58_02250"
     CDS             complement(17728..19275)
                     /locus_tag="PKH58_02250"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNX87879.1"
                     /translation="MYRKIKERMRKYLNILVITIILIFSSIKGFSTVSLLTISDSLIV
                     PVTFDTIHIQNPDTVQNSVNDSTDKITKTTPQQLTGNDHVQEIPAFKYVILQSTKTSD
                     TINHYFVNNGILKHRLDSLMKFPVKPTDSLMYSANPFFLPLVFLGKDLKPVWDGKIDY
                     MKEFYPESSTRLQLIPEKDTSTESMVFETRKIARDYISNHAAHLYETTFDQLPDPGSF
                     LQKSIRRKPVETFAFDEKGAKVDVKKIDVVKLKQRYWSPKATALMQFTQNYISQNWYQ
                     GGNSNLAILNILTGEMNYDNKKNVLWENKLEWRAGFNSVDGDTIRKISTNDDVLRYTS
                     KFGLKAGGNWYYSVSGDFSTQLFDNYKAINSTQYKARLLTPFRANIGIGMDYKYKKLL
                     SLMLAPVSFKYIYANDTVNVNPNLFGIQKGQNQLRQLGSSFRAQLDYDPTREWQIDSK
                     LTFYTDYKKVEIDLEVVSNLTVNRFMSVRVAVNPRYDNTVIMKKGENAKIQFKQLLSV
                     GFSYRLL"
     gene            complement(19434..20783)
                     /gene="rmuC"
                     /locus_tag="PKH58_02255"
     CDS             complement(19434..20783)
                     /gene="rmuC"
                     /locus_tag="PKH58_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNX87880.1"
                     /translation="MHVTLFILVAVIILITGFLSAWIVQSGKINRQKEVFEINLRELR
                     NEKNSLENQYIVSNERLNIKTDELLKVGEQLVQISALAENRGMEIAALQTVNSNLKQQ
                     LESQKKELMEIQSRLTAEFENIATKILKERSDEFSVTNHKSLSQILNPLQDKIMLFEK
                     KVEETYDKDLRDKISLREEIKKLTELNSRVSDEANNLTKALKGDVKKQGNWGEVVLER
                     VLERSGLTKGQEYETQEEIQAGDNSRQRPDVIVHLPDDKHIIIDSKVSLIAYERFSSA
                     ETPEEQNRHLKEHIGSLRAHVKELSDKNYQDGLKINSPDFVLMFLPVEASFSVAVQSD
                     NELFAYAWERKIVVVSPTTLLATLRTISSIWRQENQTRNAQEIARLSKALHDKFLAFI
                     DDMSKIKTNLDRASGAYDDALKKMKDGKGNIIRTAERIQELGGIDGKKSLPADYEIF"
     gene            complement(20796..21017)
                     /locus_tag="PKH58_02260"
     CDS             complement(20796..21017)
                     /locus_tag="PKH58_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87881.1"
                     /translation="MTKKKELKPSPNPVLLRKHRQIFTLNDKENNALNRFIEKYKVKN
                     KSKFIRETLMTEILRKLDADQPTLFDNIK"
     gene            21172..21537
                     /locus_tag="PKH58_02265"
     CDS             21172..21537
                     /locus_tag="PKH58_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNX87882.1"
                     /translation="MAEHNDLGNIGEERAQAFLRNSGYRIRETNWVAGKLELDIVAEK
                     DEMLVIVEVKTRSTEFFEHPKEAITPRKIKNLVNAAHAYIMKNNWQGETRFDVISVIP
                     KGQNFSIEHIEDAFLPPIM"
     gene            21690..22322
                     /gene="pyrE"
                     /locus_tag="PKH58_02270"
     CDS             21690..22322
                     /gene="pyrE"
                     /locus_tag="PKH58_02270"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNX87883.1"
                     /translation="MKSSEKSIAEKLLKVKAVKLQPNNPFIWASGWNSPIYCDNRKTL
                     SYPAIRNYIKVELIRTIIDLYPDVEVIAGVATGAIAQGAMVADELGLPFVYVRSSPKD
                     HGLENLIEGDLKPKQKVVIIEDLISTGGSSLKAVEAIRKDGSEVLGMVAIFTYGFALA
                     DEKFKNAKVKLTALSNYDAVLTEALATNYIADKDVETLREWRLNPSVWRK"
     gene            22365..22808
                     /locus_tag="PKH58_02275"
     CDS             22365..22808
                     /locus_tag="PKH58_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNX87884.1"
                     /translation="MTTYESDIKTISSSGEVVFNILSNLSNFNNLQNLNADAQEKLNQ
                     MLKDVEFDENSCRFTVDGVGRVGFRIIEREPFKTIKLEAENSPVAVNGWIQLVQVSET
                     DTRMKLTLKADIPTMIKVMVDSKLKKGINTIADTLVQYLTYQIGK"
     gene            22898..24232
                     /gene="argH"
                     /locus_tag="PKH58_02280"
     CDS             22898..24232
                     /gene="argH"
                     /locus_tag="PKH58_02280"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNX87885.1"
                     /translation="MKLWEKSIQVNEKIEKFTVGHDREMDIYLAKYDVLGSMAHIQML
                     ESVGLLSKEELPLLLAELKNIYHLIENDQFEIEEGIEDVHSQVELMLTRKLGDVGKKI
                     HSGRSRNDQVLVDLKLFARAEIEKTVEAVSGLFDVLTEQSEKYKNILLPGYTHLQIAM
                     PSSFGLWFGAYAESLADDLQIMLSAWKITNRNPLGSAAGYGSSFPLNRQMTTDLLGFD
                     TMNYNVVYAQMGRGKMERIVASALAGVAATLSKLAFDACLFSSQNFGFIKLPDEFTTG
                     SSIMPHKKNPDVFELTRAKCNKIQSLPQQITLITNNLTSGYFRDLQIIKEVFIPAFAE
                     LNDCMEMVSMMLQRVEVKQNILDDPKYDYLFSVEEVNRLAAEGMPFRDAYKKVGLAIE
                     AGNFKPVKDIRHTHEGSIGNLCNDRIKIYMDSIREQFNFGTVHEAVKNLLES"
     gene            complement(24236..25918)
                     /gene="gldG"
                     /locus_tag="PKH58_02285"
     CDS             complement(24236..25918)
                     /gene="gldG"
                     /locus_tag="PKH58_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HNX87886.1"
                     /translation="MKKISKSTYIILFTVIALTVVSSFFFYRIDLTSDKRYSISKQTK
                     TLMKSVREPVTVTIYLTGDLNPGFLRLKKSVAELLDELSAYAEGNISINYVNPSEAET
                     NEEREQKYAELEQKGMTATSVYDKDKEGKNIQKIIFPWLEISYKGKTIPVNLLKNIRG
                     ISGEENLNISVENLEFELTDAIRRLVNTKVEKIAFLEGHGELNETETYDISRSLSRYF
                     QIDRGIIDTDPNILNDYKALIIAKPMGPFSEKDKFVIDQYVMHGGRVLWLIDGVRVSE
                     ESLTTAGISPAIELDLNLNDILFRYGVRISPVLVQDVQSVLVPVNVAAKGQQPDFQPM
                     PWVNAPLLLTSGEHPVTRNLAPVKANFASAIEIVGDDKKLERNFILASSDNTHILQTP
                     AEISLLQMPDLKDKKYFNNAYVPVGVVLEGEFQSDFSNRMAPKEIVNPSVIQNKSVRT
                     RQIIIADGDIIRNETEGTGENLKAVPLGYDRYMNQQFANNELITNAVLYLTDQSGWME
                     LRSKTVALRLLNKKISASQKTKWQIINVVIPPVILIIFGILVQWTRRRRYTR"
     gene            complement(25931..27340)
                     /locus_tag="PKH58_02290"
     CDS             complement(25931..27340)
                     /locus_tag="PKH58_02290"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX87887.1"
                     /translation="MKKFIYLIIFGLLAFTSCREDFSGVTTDPPKIHNPQDEINSHSD
                     GLQYVFDIEAVSEVYLEITTAEWNRLLSNFDINKDNEEYIQSNCVFVKASRRDTLINT
                     GLRLRGNTSRRRPEGNTGQMHSSINPDWHHVHFTLNFDEYNKKQDLHGLEKITLKWFK
                     DDGVYAREVYCYDLFERFGVWTSPKSSYCKLYLRIKEDNKQVYFGIYQVVEQIDKQYL
                     KNRSGRFAGKDGFLWKATWGADLRNADKGRMGVEIISLTKADMVQPVYDLKTNEEYLE
                     AAKTQLAAFITDFNSKTGDDFKTWIEQRMDVPLFLKTYAVSTLCGMWDDYWNNSNNFY
                     FYFDTDNKFYFIPYDYDNTLGTSLLMTNSGTQDLLRWGSDSNPLVKKIINIPEYRALY
                     VSYFHELCNPDNDLFYYTKSIDRIKKWHTLISPFVNNDTGEDMSIKDMPASWGNRAEY
                     RLLSTTNNYFIIRAQNLPD"
     gene            27669..28430
                     /locus_tag="PKH58_02295"
     CDS             27669..28430
                     /locus_tag="PKH58_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX87888.1"
                     /translation="MGKIISIANQKGGVGKTTTAINLAASLAGLDKKVLLVDADPQAN
                     ASSGLGIDVRSVEFTVYECLIDNVPTSQAIHKTEVNNLFVLPSHIDLVGAEIELLNLD
                     KREHIMQRMLMPIKNEYDIILIDCSPSLGLITVNALSASDSVIIPVQSEYFALEGISK
                     LLNTIKIIKSKLNPALEIEGFLMTMYDNRLRSANQVFAEVKKHFDTLVFDTVIYRSVR
                     LSEAPSYGKPISLFDAESRGAINYMDLAKELINRQ"
     gene            28459..29331
                     /locus_tag="PKH58_02300"
     CDS             28459..29331
                     /locus_tag="PKH58_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNX87889.1"
                     /translation="MAKNTGLGRGLGALIDMDSVNTSGSSSINEVDIELIYPNPNQPR
                     TYFDEEALTDLAVSIRELGVISPVTLRKNDDGTYLIIAGERRFRAAKSVGLNTIPAYV
                     KTAADEQVMEMALIENIQREDLNAIEIALTFYRLMEEYKLTQDRLSERVGKKRATIAN
                     YLRLLRLPAEIQMGLKNKKIDMGHARAILGLNDPAEQLKLYEQILIKDLTVRKVEELV
                     RSITETGKPENTSDKNNSVKDLKELNDLKKQLSTIFDAKIQMSCDAKGKGKITIPFAN
                     DDELMRIMQLMDKI"
     gene            29342..30061
                     /locus_tag="PKH58_02305"
     CDS             29342..30061
                     /locus_tag="PKH58_02305"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNX87890.1"
                     /translation="MKLRKTFVIYFFLQFISLAGFAGNNDFQENSILNLAGTGQDTTT
                     LMPINIQPNSKYMNMANDIADTLAVSSLEKTKFKPNPLKSIWMGAAIPGFGQIVNRKY
                     WKLPIVYGGYLGFAYAISWNNNNYITYKNAYRDIIDKDPTTNSHIDILPRGYTIQMIG
                     ESTYTNMLKTRQSTFRQWRDLSIILSVGYYALVLLDAYVDAQLYDFDISPDLVLNVQP
                     TRIERERNSPAAYGLHCSINF"
     gene            30209..31495
                     /locus_tag="PKH58_02310"
     CDS             30209..31495
                     /locus_tag="PKH58_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNX87891.1"
                     /translation="MKKRIFIYLLFFLSIVFQTHAQSSGAKPVVTDIATEVTSDSLIV
                     PAEFSGSLDLLLHSWAVDKDNVSECSSSPNPSTSDEQYKARLMKLPHVIEMPFNQEVK
                     SFIEIYTQKNRKGLEYMLGLSRYYFPIFEEALEKEQLPLELKYLPIIESALNPVAVSR
                     SGATGLWQFMIGTAKMYGLEVNTLVDERMDPRASSKAAVKYLKELYSIYSDWHLVIAA
                     YNCGPASINKAIRRAGGKHDFWTIYSFLPAQTRAYVPIFIAANYALNYANEHNLCPAQ
                     TRIPVLTDTILVNSRMHFEQISAVLNLSVEELRLLNPQYRRNIIPGDIQPYPLCLPQK
                     YSGMLIEKKDEILAFRADELINNRRAEIITSEKETEIKAPIKRKEQFTYYKVKKGQTL
                     GGIAEKFDISVSNLRKWNSISGSKIKPGQRLKIRQS"
     gene            31514..31840
                     /locus_tag="PKH58_02315"
     CDS             31514..31840
                     /locus_tag="PKH58_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNX87892.1"
                     /translation="MEKIYYSISEVATMFSINQSNLRFWEREFKQLKPRRNEKGTRFY
                     SKDDIQLIKQIIFLINDQKLTLDGVKKKLSDKKDVVVKQQEIKERLTAIRDEIKGMIA
                     LIDNNK"
     gene            31860..32162
                     /locus_tag="PKH58_02320"
     CDS             31860..32162
                     /locus_tag="PKH58_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HNX87893.1"
                     /translation="MSSIRYEKETVEKMIRLYCRKKHKSEKELCPECAKVSNYASARL
                     DNCPFGDEKTACSECKIHCYKPEMRQRIREIMRYSGPRMLLYHPFDFVQHMLKDKK"
     gene            complement(32220..32768)
                     /locus_tag="PKH58_02325"
     CDS             complement(32220..32768)
                     /locus_tag="PKH58_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX87894.1"
                     /translation="MIKKVALFLIALLPLGAMAQNVKLGHVNSQEIIQVMPELETIEK
                     TLKELQDQWEKELLKMREEYNTKVKEFVDKQATMPETIKTARQSEIAEIEQRITTFNQ
                     TANADVVKKQQELVNPVLEKVKKAISDVATEGGYTYIFDISTQGVVFQSKTANDITPL
                     VKKKLGVKDQPAVVAPAAPANK"
     gene            complement(32833..33348)
                     /locus_tag="PKH58_02330"
     CDS             complement(32833..33348)
                     /locus_tag="PKH58_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX87895.1"
                     /translation="MKKLFITLSLLAGLAIGVQAQKFAAVDMSYIMKNIPAYESANEQ
                     LNQVSKKWQTEVETAMKDVQQMYKNYQTELVFMSDDMKVKREDEIVAKEKEAQELKRK
                     YFGPEGELFKKRESLMKPIQDEIYTAIQEISKDKDLQLVFDKSSAMSAIFVSPKLDIS
                     DDVLKKLGYSK"
BASE COUNT         9773 a         7309 c         6499 g         9942 t
ORIGIN      
        1 taagcattgt aatttatttt agttacgaga tcgaaatttt cgggtgtcat gtcttccaga
       61 cttcctgctc gcagcactcc tgcattgctg atgaaacaat ccacggctcc aaaattgcat
      121 attgtttgct gtatcagatt ctcgatactt tgtatttcgc ttacgttagt ctttacaaaa
      181 atggcacggt tgcttttggc tatttcatta aagtgagcta cggtggcatt tccaacctcc
      241 tcgttcaaat ctgccaccac aatattggca ccctgctgta aaagattgcg tgcaattcct
      301 tcaccaaaac cctgtgctgc accggttacg ataatggttt tgttttcagc acgacccgta
      361 acacctcctg cagcaacact ccggcggtaa ttttccactt cccagttatc gataaaatct
      421 atctgagcct gtgtcatagg gtgcgcacct ccaaatgact gtgagagggt ggcaattttc
      481 attgcatctt caaacacatc cagaattatt tcgcattgcg cagcattatc accagccgca
      541 accaaaccaa ttcctttgat taataacact ttgggttgat aaccaaattt aacccgaaag
      601 ttttttattt ctttttcaaa ataggagatt atttcttcag gagttaattc tcccccttta
      661 gggggttggg gggcaaatat ataattcgat ttgcaataaa caatggcatc aggagtaaat
      721 ggtcgggcaa tattccgttg attttcggaa ctttcggaaa aatatttcac cagttcgtta
      781 ttgcgaactt tgagggtttt caaaccatca tcagaaagca tcatgcggat agcaggaaga
      841 atttcagccc ccttacctcc tgaaggtgga atacttactc cccctttagg gggttggggg
      901 gcttctttat ctaatgtatt aaatatttct tgataaatac attttatttc atctattgta
      961 tcggcagcaa caaaaattcc gtggttttgt aaccagatca ctttcggttc ttcattgaat
     1021 tttgcgcgaa atgcttttat tttgtcatcc acttttttga ataaaacata tcccgggtca
     1081 gtatattcaa cataaagtgt tttctctcca aagagtttta tcaaatcggc ttctgcattt
     1141 tgcgaacaca tcaaaccgtt tactgccgta ggatgcaggt gaactacaaa tgcataattg
     1201 atcacgttgt gcatggaagt ttctaccgac gggcgttttc ctttggtaat ggttgcatat
     1261 gccaaatcat ttttcacctg ttcttcgcgt tcgcttgtgt cggcgctgta cactttttcc
     1321 gacatcaggt ttaatttttc acgatccagc accgcaaaac cttcttccgt aatggttgcc
     1381 aacgatgacc cactggcttt cacccatatt ttttcctgat ttttatacga tgtgtttcct
     1441 cctccggcaa taacaaaacg gctgtccttc ccgtagaatt gagagatata aatcaacccc
     1501 ccaaccccct gaagggggag ttcagaactt tgacttatat tatctttatt catattaaat
     1561 tatcaatatt tttattttta tcacctctat aaacgctctc taattccttc ttcagggggt
     1621 tagggggctg ttataagttc tttccccaac ttcacaaatt ctgctctatt tgatcggtct
     1681 gcgtttccct tttcgtccac gtagccttta aaaccctgtg caacaagatg ctggtgtgct
     1741 gcaaccacac acgcaccttc aaagtttatt tgcatcagtt tttcagtcag tggtgtgttg
     1801 tttcttgcaa aaagttcaac cggttccatg accggtgtat tgtgctcgct tccaaacgtt
     1861 acaataaatc cctggtcaaa aagatattca gcgtattttt ccagaagact gacatcgttg
     1921 cgtgtagtta taaactccac cgaatgaaat ccgcgttctg ttaattgctt tgcaacgcgt
     1981 tgcaggtcgt tttcaaaatc ggtatagcct cctttggcat catcggcaag aaacggatag
     2041 gtaggaatac caccacctgt caggataatt tcgcaaactg tctgcatcgg caaaaatgct
     2101 ttggcatctt ccgggacaaa tgccgcgcca cccgctttca gaagatttgc acgaatttcg
     2161 ttttcaacgc cagccacatc atttatctca gatttcagct cctttccacc aaaaagtttc
     2221 gtgaaaagcg atttgatttc ctcctcatta tcttcataaa tttcatacac tttcattctg
     2281 agtgcctttg ccagatgacg ctcgcggaca gaaccctttg tgagttcgtt tttaatccat
     2341 tcgaaatcga gcgaaaaacc ggcattgttt gttttcagca gttcattcag tttaccacac
     2401 atggctttca cctggttgtt agcctctttc cggacatctg caagcagcga tgtataaggt
     2461 tcaaccaagc tgaacgggta actcatccct tttccgctca gataggttct acctggattt
     2521 ccggggtcgt tcacccgaag tccggctttc tggtcttctt cattcaggct gataaattca
     2581 atcccgaaca gtggaaacaa agctcgattt ttacagccga ctgaccattc ttcatatcct
     2641 gcggtggtgt aaaaatcatt aattcccacc actttcaccc cttcggcaac tgctctgtcc
     2701 agcgcatcgc tgatatcgct gaaagcgcta aaagagtatg gtgtatgcaa gtgggcatta
     2761 actttcatgt aactcataat atgcatttta agattatttt tcactgaatc tgagaacatt
     2821 ttcgtgaata tgttttttag aactaatggt atcagcagca atattcttta attttacatt
     2881 ctcaacaggt aattcctttt gtcccagtat ccgcgaaatg aattccactt tgtctgcctt
     2941 catgtttttc aaactgatat tctttatcgg agtaagtcgt ttttcaattg taggaacaag
     3001 atcacgccac tgatacagca catccgtttc aattccgagt accccgtttt gtacactacc
     3061 ggctttaata ttctccacat aaatatttct cacataccca cccattcttt cgtttgtctt
     3121 aatgaatacc agatggaaca tttttgcacc ttttgatacg gtgcaatttt tcaggcagat
     3181 gttttcgata ccaccggaga gttcacttcc tatagccagc aactgatgtc cgttttttac
     3241 cgtacagttg cgtatgacaa tattctttga aggtgtgcgg gtgcgccagc cttcgggact
     3301 acgacccgac ttcactgcga tggcatcatc tccctgatcg aacgtacaat tttctatcag
     3361 tacattctgg ctcatttcag gatccacacc atcgttgttg tgtccgtgag catatacgct
     3421 gacatttcga attacaatat ctttggacaa ataaagatgt atcgtccaga aagggctgtt
     3481 tattatatta actccttcaa tcagaacatt ctgacagcgg ttcatttgga taaattgcgg
     3541 acgcaaattg gaagagtcgt ttaccataat cctctcattc accggtttat tgtcgaaagc
     3601 ccagtaatac agatttttca ggctgttcat gtgcggtctt ggccgtcctg cccatttttt
     3661 ccagttatcc atcagagctt ttacctgacc ttcgccggta attgccacat tttcacactc
     3721 atatgcatat atcaacggcg aataattgta acattccaat ccctcccatg tagagtgaac
     3781 tgccggtaaa tattctcccg gatcatcgga aaaaagcagg actgctccct tcatcagatg
     3841 tagattgaca ttgcttttca gatgtatttt accggtaatc cattcccctt cgggcacaac
     3901 aactgtgcct ccgccggaat tattagcgac ttcaatggct ctagctatcg cttgcgtgat
     3961 tttagacttg tcgccctgaa ctgctccaaa atcagtgata gtatatgttt tacacttcga
     4021 aaaatcaggc acacgaataa ccggcatctg aaagggagct ttgaccttaa tacctgtggt
     4081 cggcacttcg ccaattacct gttgcgtaac aaggagctgt agcaacagca tcagcattag
     4141 ccataaccct cctgtgttct tcatatgtga atatttcctt taatgattga ttgcggagat
     4201 gttcatttaa tatatcaagg cactcatcct ttgtcagtta agtgccttaa tataattatc
     4261 tatacccctg atttcgagcg gcgaataagt tcggcactgg taaaattctg tccgggtatg
     4321 taatccttca gtggctggat tcgttcatga ataacatcga ggaaccaggt tggctgcgcg
     4381 aagaatgaac tttcaagttc gtaagcaggt gtaatccagt tgcgcgaacc aagtacacat
     4441 accggagcat ccagataatc gaacgccagc tgaccgatat ttgccgccag atcgttcagg
     4501 aatgaaccac gggtagttgc atcgcttgcc acaattattt ttccggtttt ctttaccgat
     4561 gcaatcactt tttcgtaatt gaacggtacg agcgaacgtg catcaataac ttcggccgtc
     4621 attccgtatt tttcttccag aacttttgct gcctgcaaag ccggatacag cgtatggcca
     4681 acagtcagga aagttatatc atttccctct tttttcacat cgggttcacc aaacggaatt
     4741 tcgaaatatc ctgacggaac tcctcctttg tggaattgtt ccccgatttc gtaaatacgc
     4801 tgactttcga agaaaatcac cggatcggtt ccctgcaaag cagcattcat cagccccttg
     4861 gcatcgtaag gagtgacagg gaaacatact ttgaggcccg gaatttgagc aacgagcgaa
     4921 gtccagtcct gtgagtgctg ggcaccgtat tttgagccca ccgacaccct caccacaacc
     4981 ggcattttga gcacatttcc gctcatagcc tgccatttgg gaagctgatt aaacacttcg
     5041 tcgccgcaac gtccgagaaa atcgcagtac atgatttcag gaatcacccg tccgccacac
     5101 atagcgtaac caatggctgt tccgatgata gatgcttcag aaatggggga gttgaacagc
     5161 cgatgatatg gcaatgcttc ggtcattccg ccataaactg caaacgcacc accccagtcg
     5221 cggttttctt cgccgtaagc tatcagggat gcatccttgt agaaacgatc cattatggct
     5281 tcgaatatac cgtcgcgaag ctgatattgt ttcattttgc tgaaaggttt tccatcggca
     5341 tcgaatgcaa accgctcctt ggctgcaatt ttctttaccc gtgaattttc ttcaagcggc
     5401 atcaatacat ccggttttgc atcggagaag ctttccaccg atccgttgga gaacatcata
     5461 tcgctgatca cctccggatt tttcatacgt ggtgacacct catcgtcgat tgctttcaag
     5521 aacatttcat aaaccaactc cttaacatcc gcctgaatag catccaattc atgctgatct
     5581 gtcacttcag cttcaatcat ttgtttaccg aaagaagcta tgcaatcctg gttttcccag
     5641 gcttccactt cttccttagt gcgatacgat gaggcatccg agggcgagtg accactgtaa
     5701 cgataggtta atacatccag caaaaccgga cctttcttat cttcaattat ttttcgttta
     5761 cgcttgtaag catcaatcac ggcaagcgga ttgtagccat ccacacgttc agcatgcatc
     5821 tgttcgggat taacaccggc accgatgcgg gctgcaatac cataacccat tgtttcgccg
     5881 caagtctggc ctcccattcc gtactgatta ttcataatgt tgataatcac aggcagacca
     5941 cccttcatat cgccttccca cagattatta aactgatcca tggcagcaaa tgtaatgcct
     6001 tcccacacgg gtccgcaagc catggaagca tcaccgatat tggctacgac caaacccggt
     6061 ttccggttca cttttttgta tagtgcagca ccaacggcaa tatcgcctga accacctaca
     6121 atggcattgt tgggatatac accgaaagga gtgaagaatg catgcataga tccgcccaaa
     6181 ccgcggttga agcctgtttc gcgggcaaaa atttcggata gtgtaccata aaccagaaag
     6241 cgtcgtgcca gttcctttgt tgtaccgttg aaatcctttt tcacaacatt cagtaccgca
     6301 ccatcaaaga aactttccat aatcttcaac agctcatcat catctgtttt atgaatggcc
     6361 gacatccctt ttgccagtat ctccccatga ctgcggtgac ttccgaaaat aaagtcgtcg
     6421 acagtgagtg tccaggccat tcccacagct gccgattctt gcccgatgga caggtgagca
     6481 ggaccgggat ggttatacgg agttccgtta tactctcctt tcgttttgat gagattgagc
     6541 atgatttcaa attcgcggat aagggccatg tcgtgataaa tggctttgaa ttcttcatcc
     6601 gaaaaatttc ctttttcatc cttaaccgtt ttctgatact gattgaccgg aattggctga
     6661 aattcaacga aaccgggttg acgcgcttgt gacgggtcta taaattgtat ttttggcatg
     6721 gtgtaatatt ttttttactc tttttatcac aaaagtcact aagcctctta aaaatataac
     6781 ctcttgactt agtggcaaat tattatattg agaatattgt ttctttaaat atttcgctga
     6841 cggtaggatg aggaaatacc acttcctgca attcttttac tgtcatttcc tgttcgattg
     6901 ccatacaggc accgtaaata atttcgctac agggatttcc cagcatatgc actccgataa
     6961 cttcaccgtg tttttcgcca accagcactt tgcacagtcc attgccacct tcattctcgg
     7021 ccacaaaacg tccggcataa ctcatcgcca gttttattac tttgtaagga atgcctttgg
     7081 ctttggcgct ttcttcggtt tcacccacac ccgctacttc tggattggtg taaacaaccc
     7141 cgggaatagc attgtagcgc ataacatcat ttcttccggt cagattatta actaccactt
     7201 caccttcgcg acttgcagtg tgagccaaca gtgaaaatcc ggttacatcg cctgccgcaa
     7261 acactccggg cacattggta tgcattttct cgtccacttt gatgcctcca cgtgtcatct
     7321 ctacattcag attttccagt ccgtaacctt tcaccacagc ccgacgtccg acactcagca
     7381 ggattttttc tccctgaatg gtttcggtct ttccgtcttt ctcaaacacc acggtatttc
     7441 cttccacccg aacgactttg gcattgaggt gaaattcaat tccttttttg gcgtaaatat
     7501 cgcgcagtat agcagaaacc tcaaaatcca gtccgccaag tatttccggc aacatttcaa
     7561 taattgttac ttttgtacca agactgttat agaaacttgc aaattccatc ccgattaccc
     7621 ctccaccaat tacaactaat gtcgccggtt gctctttcag ttcaagaatt tcgcggttgg
     7681 taaggatgac atctccggct tccgccaatc caggaatagg tggaacagac gcttcggagc
     7741 cggtacatat cagcaggttt ttagccagat aactttctcc gttacaattt acttctacac
     7801 ccttttcgct gcgtccctgt atgattgctt cgccttcaat aacggttaca ttattctctt
     7861 tcatcttgct tttcaccccg gcaaccagct ttctaaccac tttattttta cgtgcaacta
     7921 ttttcccgaa atcgaagcgg acattttcgg caaaaatgcc atatttatct ccatgcaacg
     7981 cattttcata ggtttttgcg ctgtaaagca gggttttggt aggaatgcat ccttcattca
     8041 gacaaacgcc acccattgat ttcttttcga ataaaataac attaagtcct ttgtgaccag
     8101 ctctttctgc tgccacatat ccggccggac ctccgccaat aatcattaaa tcgtacataa
     8161 atttgccccc taccccctaa agggagaatt ttggctctta agtttgccct cttatcacct
     8221 aaagaggaaa tatgaaggcg agacaaaaga gatttgtgtt aattattaat atttcttaca
     8281 aaaatagtat tatatttcta taaacccctc agagatatat ctttggatac tttattatat
     8341 gtttgtgaca catcttttcc tcccttaggg ggttaggggg cctatagcac cagttcaagc
     8401 gtttcaatct caatggcaat ctgcttcagg aaccgggttg cttcgccacc atccagtgag
     8461 cggtgatcat aagtgaggct cagtccgata tagggcacaa aagcatacac gccatcgccc
     8521 agatctttcg gacggggaac aatggtattt acacccaaga tagccgactg cggcagattg
     8581 actaccggag taaacatttc cacgccgtaa ttaccaagat tggaaaccgt aaatgtagca
     8641 gcttcagctg atagcaactc gggattaata cttcctttcc tgcaggcatt tgccatttct
     8701 ttgaattgct gagccaaacc gccgatggtt aaatcatctg cattacgtac tacgggtacc
     8761 atcagtccac gttcggtatc aacagctaat gccaggtgaa ccttgttgaa ataacgtact
     8821 gtatcaccca gaaaatgagt atttacattc gggaattttt tcagagcctt aatcaccgca
     8881 aaacaaacca tatcgttcag cgtgatattg gtttcgagtt ttccggcttc catagcagtt
     8941 ttcacctgtt tgcgcagttc aaggatgcgt cgtgcatcag ctccgaggtg atgagtgagt
     9001 tgcgcagaat tctgaagcga tgcgtgcatg gatttggcaa tgattttgcg catattggat
     9061 atttttttat cctcataatc tgcaacgcta cctgcattta ccggaactga taaatcatgc
     9121 gaagtgattg tcccggccaa accattaccc caaccctgtg gctgcatacc ttcctggata
     9181 actatttttt tagccagcga agtgagtttc gggcggtttt cgagtaccga ttgtacatcc
     9241 ttttcaataa tacgtccgtc ggggcctgat cctgaaatct gtgaaatatc caacgcttcc
     9301 ttttctgcca gatttcttgc acggggtgac aaccctcctg ttggtttcag ctgacttatt
     9361 attacttctt cctgtatttt tatttctgtt tttgctgtct cctctttttt cacgtcggct
     9421 tctgcatgcg gtttcgtgtc tgtaagcaga tcaaggggaa cttcttcgcc gggctgtccg
     9481 ataaccatca cattatcaag aacaggcact tcatcgcctt cgttacaaaa acattcgagc
     9541 acgactccat cagccgtaga ctcttcttca aaggaagctt tatcggtttc gtacgaaaac
     9601 aagatgtcgc ctttctttac aacatcacct ttctttttga tttcggatat gatgcaactc
     9661 tcgaccgact gtccctgttt gggcattaca ataattatag ccatgaattc ttatattatt
     9721 tcattcaatt ttgacaaatt aatttgcact tataataaag taggaatgac atcagtcaac
     9781 aatctgtcat tcgcaatccg acatttattt ttcagccaac aaaattaaaa tttatagaac
     9841 caaatatcag tatattaaat gattttttag cagtgatttt tgacaaaaca gctaataccg
     9901 gtaaaataat accttttata caagtaagaa aattgcagct aattaacatt ttatatccgg
     9961 taaagcgact gctgctttta cgaatatcca tttatttaaa ttaaatactt atttttgttc
    10021 tttaaccata aaaattttaa tatgattgca aaatttcaat taacattact gttttctgtg
    10081 atattgattt tttattcaaa tgctcaaaac acaatctcaa cgccacaggc aacaaacaga
    10141 gaaaataatt caaagttatt aagacaaact accggtaatg aaccggataa ttcaattaaa
    10201 ccgaatttaa acatcaactc tgattttctg cacccccaaa gccggcttat atttccttcc
    10261 cggttaaatg actttcagcg aaaccggatt attttcaccc aaaataatcc ttcaaaaata
    10321 gatgtcattt actactcagc agactctgga aatgaaatcg ccatcagttt gtatcggtac
    10381 gaaacgggaa cagaaggtcg tctgcggaat gaatatctta acgctatcag aaaattgaca
    10441 aaggaagaga atgtggattc tattcccagg gcaatccctg taaagtacaa aggtttgaaa
    10501 tacatttgca atggagtgaa aggcagatat gacttttcag agacagcaca tactgaactt
    10561 tgcgtatatg aatgcgggac ctggatgatg aatataaaaa tcaggagtaa aaatctcaat
    10621 tcgaatgaac tgatagaact ggaaaaaatt cttacaaata cctttgatcc ctcgtggcta
    10681 acatcgcaaa atccactgtt gctgaaatcg aacgttgatt ttgagcgatc ggcactggca
    10741 gataccgtca tgactgccgc gatggtggca agtgctttta aaaaacttga ctgggcaaca
    10801 tacaacgtaa gggcaaacga acgggcatcg ggtttcccgg atctttatct caatatgcat
    10861 attgcatcca ttcgggaatt tatacaaatt cagcagaaga aaccacgcct gtatgtaagc
    10921 ccgtcagcgg agaaatattt caaagaactg agcgaaataa acaatgcagg ttttctgcct
    10981 gaatttatca tggaacagta tcagaacatc atgtttgtac ctgatggtca ggttctgaac
    11041 cacgaaggat ataagaaatg gaaagaaaat aaaaatctga ctattgacct gaacagaaag
    11101 atgtatacga tttgctaccg aaaacagtaa aaaaatctga aaaggaggta ctttctatca
    11161 gaacgattgc atatcgacca gttttttata gtagcctccc tgtttcatca actcttcatg
    11221 cctgcccctt tctacaattt tcccctcatg cagaacgcag atcagatcgg ctcttttgat
    11281 ggtggacaaa cggtgagcaa ccacaaccgt cgtgcggttt ttcatcagat tatccagtgc
    11341 ttcctgtaca agatattctg attcagtatc cagcgccgaa gtggcctcat ccagtattaa
    11401 tatcggagga tttttcaaaa tggcacgcgc gatgctgatg cgctgacgct gcccgcccga
    11461 cagtttgctg ccccgatcgc cgatgcaggt ttcataacca ttttcagtag ccacaatgaa
    11521 atcgtgggcg ttggctattt ttgcagcatt gataacttgc tcgcgcgtag ccagttcaac
    11581 accgaaagct atattattat aaaatgtatc gttgaagagg attgcttcct gatttacatt
    11641 tcccatcagc gaacgtatgt ctgactttct ataatcttta atattaacat tgttgaaaaa
    11701 aatacccccc tcagccggat cataaaaacg gggaagcaaa tcgaccagtg tggattttcc
    11761 ggaaccggac tgcccaacaa tagcgactgt tttacccaac gggattgtca gattgatatg
    11821 ctttaaaacc cattcggact gatacctgaa actaaggtct ttaaattcta ttttctcaat
    11881 tatggcgggc agcatagcag gaacctccgg gtcgggtata ttattctcct gcatcaggat
    11941 tttatcaatc cggttgagcg aagccatccc tttctgtatg ctgtacgatg cttttgaaag
    12001 ttcttttgcc ggaggcacaa tgctgtaaaa tatcaccaga tagtaaataa actcggcggc
    12061 accgatgctg ctgttctgtt gcagtatcag actacctcca aaccatataa gtatagcgat
    12121 gacaatggtc ccgaacaact cgctgaccgg atgtgccaga ttatattttc ggtgaatacg
    12181 gttgaaagta tgccggattt tttcatttaa tattccgaaa cgttgtgtaa gtttttcttc
    12241 ggcattgaaa gcttttacaa ccctgagtcc tccaagtgtt tcttctatct gcgaaagcag
    12301 ttcaccagtt tgctcctgcc ccagggtcga tcgttttttc agagaacgcc cgataacacc
    12361 aatcaaccag gcgcttacag gcagtaaaac aagcacaaaa agagttaact cccaactcat
    12421 ggcaaataac acaatcaaat aaatcagtat catcagcgga tttttcagca ccatatccag
    12481 cgagctgatg atggaagctt ccacctcaac tacatcacct gtcatgcgag acattatatc
    12541 acccttgcgt tcatccgaaa aaaagccgat gggcagcgaa atgatctttt tatacatttt
    12601 gtttcgcaaa tcgcgcaaaa ctccggttcg gataggtatg acaaaataag aagccagata
    12661 ggtggttcct gttttgaaaa aagtcattat aatcagaaaa agccccagat aaagcaaagc
    12721 cattccgggt cccgcgcttt ctataagttg ctgcacgtag taccccagat tgttttttat
    12781 tgccgcgaat acagctgaaa gcccatcatc ccagttccac gaaacataaa ctgcatctgc
    12841 tttcgaaagg ccaaagagta tctggagcac gggtatgata gctgcaaacg agaagaggct
    12901 gaatatggtt gatattaaat tgaagaccaa actcagaaaa atatatttct tgtagggtgg
    12961 tgcgaaccgc ttaataagcg tcaggaaatc aaacatttcg gattgaatat tttattacta
    13021 aaaatttttt tgaccagtat tttaaattcc tgtatagttt cgtggagtaa cacgacggag
    13081 ttcctccttc acagaatcgc tcacatccag cttatcaatg aattccctta tagctttttc
    13141 gttgattgct tcgttggtac gcgtcaacgc tttcaaagct tcgtaaggtg acggatattc
    13201 ttcccgccgg agaatggtct gaataccctc agcaacaacc gcccaggtat tatcaagatc
    13261 acggtacagg gcagtttcat tgagcaataa tttatcaagt cctttcagga tgctttgagt
    13321 agctatcagc gtatgagaga atggcactcc gacatttcgc agcacggtac tgtcggtgag
    13381 gtcgcgttgt aagcgcgaaa cgggtaattt ttgcgccagg aaagtgaaca tagcattagc
    13441 aacacccagg tttccttcag cattttcgaa atcaatcgga ttaaccttat gtggcattgc
    13501 tgaggaaccc acttcaccgg cttttatttt ctgcttgaaa tactccatgg aaacataggt
    13561 ccagatatcg cggcaaaaat caattagaat tgtattgatt cgagccaggg catcgaataa
    13621 tgcagccaga ttgtcgtaat ttgaaatttg tgtagtccac tgctcacgtt ccagaccaag
    13681 tttttcgagg gcaaacttgt tggcaaataa cttccagtca atattgggat aagcaacatg
    13741 atgtgcatta aaattgccgg tagccccacc aaacttaacg gactgaggta ctgctttcag
    13801 caactcgagc tgtttattca ggcggctgac aaaaaccata atctctttac ccaaacgtgt
    13861 aggtgaagct ggctgtccgt gagttttggc cagcatggac acatctcgcc attcgtcggc
    13921 catgcggcgc agttttaaaa tcagattgtc aagttccgga taatagactt cttccatccc
    13981 ttttttgatg gaaagcggaa ccgatgtatt gttgatatcc tgagacgtaa gtccgaaatg
    14041 aataaattca cgataagcac ccagaccgat ttcattaaat ttctctttta gaaaatattc
    14101 aacagctttt acatcatgat ttgtaacctt ctcaatatct ttgattttct gagcgtctcc
    14161 gatcgaaaaa ttttcataaa tttctcttag ttttggcaga atatttacgg gaacagattc
    14221 cagttgtttg agcggcaatt cgcataaagc aataaaatac tcaacttctg tcagaacgcg
    14281 gaaacgtata agggcaaatt ctgaaaaata ggcggcataa gcttcggttt tggaactgta
    14341 acgaccgtca acaggagaaa tagctgtaag tgaattgatt gtcataattg atagggattg
    14401 aattcggatt atttgttttc gaacaacaat aatgctacaa agttaatcaa atttgagata
    14461 actgaaaaat tcagatgtca gcttgttgat ttaattttaa atgcttactt ttgttagtta
    14521 atactaaacg tttcttacag gctaccatgc ttacccatca aattaaaaat caatgaaata
    14581 cattaagata cataaagaag gaaaaatgat actggcattt acactggttg ttttactggt
    14641 tattaatgtt gtagtctata tgcaatttcc taagccattt cttgcaatca acattactct
    14701 gtctgcattt ttactgattt tcgtcatgtt ttttttccgc aatccgcgcc gggttgtcga
    14761 aattgacgat ccaagcctga ttatcgcccc agccgatggt acagtcgttg tgattgaacc
    14821 tactgatgaa tttgaatatt ttggagataa acgaattcag gtttccattt tcatgtcgct
    14881 ctttaatgta catgccaact ggtatcctgt tgcaggaaaa gtaatcaaga cggcacatca
    14941 cagcggacgg cacatggccg cttatctgcc taaatcaagc accgaaaacg aacgatcaac
    15001 cattgtaatt gaaacaccgt caaaaacaca gatactggtc agacaaattg ccggagcgtt
    15061 ggcacggcga attgtaactt atgcacacgt tgggaaaacc tgtcacctga atgaacatat
    15121 aggctttatc aaattcggct cccgtgtgga tatgtttctg ccactcgatg ctgagatttt
    15181 tgtagaaatc ggagaatcta ccaccggcaa cgagacgatt attgcacgcc ttaacgaaga
    15241 ttaaacatga cattgcagga agctcaaaca cgggtggacg aatggattaa aacatacggt
    15301 gtccggtatt tcagcgaact gaccaatttg ggaattttaa ccgaagaagt tggtgaactg
    15361 gcacgtatca tggttcgcac atacggcgaa caatcattta aggaaacaga taaaaacaac
    15421 tctttggctg acgaaatagc cgatgtgctc tgggtgctga tttgcctggc taatcaaacc
    15481 ggagtgaacc tgactgatgc ctttgaaaaa aatctcctca agaaaacgaa tcgtgacaac
    15541 aacagacatt ttgaaaatga aaaattaaaa cattgaatac agaaagatta tgaacaaatt
    15601 tgaagaatta tttaatgcat ataactgtga cacactcacc gacgaaacag taaagaaaca
    15661 tgtagagcag atacttacag gacatttcga tgaaaacaac aacatcgggg tttataaaca
    15721 gtgttttaat cagatagacc tcacatccct tcgtgaaacc gatactgaag ccgaaatcac
    15781 agctatggtc gaaaaagtga ataattttga caacaatttc cccgatattc cgaatgtagc
    15841 tgccatctgt atatatccgg cgcttgttcc ggttgtgaaa gcatctctga ccgaaccgat
    15901 cggaattgct gccgtatcgg ccggtttccc cgcatcacaa acatttgtgg aaattaaagt
    15961 tgccgaaacc tcaatgtgtg tcatggaagg tgcaacagaa attgacgtgg ttatttcagt
    16021 gggtaaattc cttgaaggta aatacgacga agtctatgaa gaacttactg aaataaaatc
    16081 ggcctgccgg gacgcccacc tgaaagtgat actcgaaacg ggtgcactta aaacagcatc
    16141 aaacataaag aaagcagccg tactggccat gcaggcagga gccgatttca tcaaaacttc
    16201 aacaggtaaa attgcggtag cagccacccc tgaagccacc tgggtaatgt gtcaggcaat
    16261 cagggaatgg aacgaaaaaa ccggtataaa agtgggctat aaaccagccg gagggatagc
    16321 aaataccgat gacgccgtaa aacactacac gctggtaaaa gaaattcttg gtaacgaatg
    16381 gctcgacaac aagttattcc gcttcggagc cagcagcctg gcaaacaaat tgcttacttc
    16441 aattaataag gaagagataa aatatttctg ataatcggaa agattttatc gaagaaaatt
    16501 tattccataa aaaagtagtg tcagaatcat gatactgctt tttttttata tattttttta
    16561 ccgatatttg taatcggatt aaaataatta tattcagtat gaatactttc gatttattta
    16621 tcttcatacc catcacatta ggtctgattt tcggactttt caggggcttt gtcaaagaag
    16681 tggtttcact ggtggcagtg atactggcaa ttgtagcagc tgaaatattc agttcgttat
    16741 tttccgattt tcttgctggt ttatttaatg tttccaacaa agtatctgca acaatggctt
    16801 atgtttttgt atttattatt accattgtca ttgcactgct tattgcccga atggctgaga
    16861 aactgatatc aaatattagt ttaggttggc ttaattcgtt gctgggaggt gttttcggcg
    16921 cattgaaatt tgcctttatt ataagtgttt tgatgaacgt ttttgatgca atcgacacaa
    16981 aatttcatct ggcaaaacct gaaacaaaaa tagaatcagt cggttattat ccggttctga
    17041 aactggcacc ctcgctctgg aaacattcga aagaagccta tcagaaaagt aaaacttctg
    17101 aaaatgaagg ccaatcaaca tcgcctgaaa atgaagcaaa ctaatctgcc gtcgaattac
    17161 tccggacaat ttcccgaatg cggctgtgat gaagcgggtc gcggttgtct ggccggccct
    17221 gtagtggctg cagctgtcat tttgccacct gattttgaat gtgccgaact gaacgactca
    17281 aagcaactca ccgaaattca acgggatgcc ctcagaatta tcattgaaaa agaagctgta
    17341 agctggagcg tagcaatggt gagtgaaaag gaaattgatg aaattaatat actaaatgcc
    17401 tctatcctgg ctatgcatcg ggcaattgac aagctggcaa tcaaaccctg ctttattatt
    17461 gttgatggaa ataaatttaa aaaatatcag gaaattcccc atcagaccat tgtgaaaggt
    17521 gatagtaaat atttgtcgat cgcagcagct tcagtgctgg caaaaacgca ccgggatgaa
    17581 ctgatgatga aacttcacga cgaatatcct caatatgcct ggaacaaaaa caaagcttac
    17641 cccaccatta aacaccgaga agcaatagcc agttacgggg taacgccata tcacagactg
    17701 acttacaaat tactgaaaga taataactta tagcaaacga taggagaaac caacactaag
    17761 cagttgctta aactgtattt tggcattttc tccttttttc attattaccg tattgtcgta
    17821 gcgaggattg actgcaacac gcacagacat aaagcggtta actgtcaggt tgctcacaac
    17881 ctccaggtca atttccactt tcttgtaatc ggtatagaag gtaagttttg aatcaatctg
    17941 ccattcgcgg gtgggatcat aatcgagctg tgccctgaac gaactaccaa gctgccgcaa
    18001 ctgattttga cccttctgga taccaaacag atttggattt acattcacag tatcattggc
    18061 ataaatgtat ttaaacgaaa ctggcgctaa catcagagat aagagcttct tatacttata
    18121 atccatacca atacctatat ttgcgcggaa aggagtcaaa agcctggctt tatactgtgt
    18181 agagttgata gccttataat tgtcgaataa ttgtgtggaa aaatctccgg aaacactata
    18241 gtaccagtta ccgcctgctt tcagaccaaa cttggaagtg taccggagca cgtcatcgtt
    18301 ggtacttatt tttcggatgg tatccccatc gaccgaatta aatcccgccc gccattccag
    18361 tttattttcc cacagaacat tctttttatt atcgtaattc atttctcccg tgaggatatt
    18421 caatatcgca agattcgaat tgccaccctg ataccagttc tgcgagatat aattctgcgt
    18481 aaactgcatt aaagcagtcg ctttgggact ccagtaccgc tgtttcagtt ttacaacatc
    18541 aatttttttc acatccactt tagctccctt ttcgtcgaat gcaaatgttt cgacaggttt
    18601 acgtcttatc gacttttgca aaaaagaacc tgggtcgggc aactggtcga aagtagtctc
    18661 gtacagatgt gctgcgtgat tcgaaatata atcgcgtgca attttcctgg tttcaaagac
    18721 cattgattcg gtcgatgtgt ctttttctgg tatcagttgc aaccgggtcg aactttccgg
    18781 ataaaactcc ttcatataat ctattttccc gtcccataca ggtttcagat ctttacccag
    18841 aaaaactaaa ggtaaaaaga atggattggc cgaatacatt aaagaatctg ttggtttaac
    18901 gggaaacttc atcagggaat ccagtctgtg ttttagaata ccattattga caaaataatg
    18961 attaattgta tcagaagttt tggttgattg caaaataaca tatttgaatg caggtatttc
    19021 ctgaacgtga tcgttgcctg tgagctgctg aggggttgtt ttcgttattt tatcagtcga
    19081 atcatttact gaattctgga ctgtatccgg gttctgaata tggatagtat caaatgtaac
    19141 gggcacaata agtgaatcgg aaattgtcag taaactgaca gtcgaaaaac cttttatgga
    19201 actaaatatt aatattatag ttattaccaa tatgttcaaa tactttctca tgcgttcttt
    19261 tatttttcta tacatggttc gtaacttttt atcatctcac tgttgctggt aaggcaccat
    19321 gctgttgact gtttgttttt ctgaatatta aactgcggaa tacaaacaga tattaaacct
    19381 ttcatatcaa atttcaagta atcgtatggc tatttgaaac gccctggggt ttgtcaaaat
    19441 atttcgtaat ctgccggcag tgattttttt ccgtcaatac ctccaagttc ctgaatccgt
    19501 tctgcagtac gaattatatt tcctttgcca tctttcattt tcttcagagc atcgtcgtat
    19561 gctcccgaag cacggtcaag gttcgtttta attttcgaca tatcgtcaat aaaagccagg
    19621 aacttatcat gcagcgcttt gctcaggcgt gcaatttcct gtgcgttacg ggtctgattt
    19681 tcctgtcgcc agatggatga aatggttctc agcgtagcca gcagtgtagt ggggcttacc
    19741 actacaattt tacgttccca ggcataggca aacaactcat tatctgactg aaccgccacc
    19801 gaaaatgaag cttcaaccgg caaaaacatc agcacaaaat caggtgaatt aattttcaat
    19861 ccatcctgat aatttttatc actcaattcc ttcacatgtg ccctgagcga accgatatgc
    19921 tctttcaggt gacggttttg ttcttcgggt gtttcggctg aagaaaaccg ttcataagca
    19981 attaacgaaa ctttagagtc aattattata tgtttgtcgt cgggcaaatg aacaattaca
    20041 tcgggccgtt gccgcgaatt atcacctgcc tgaatttctt cctgagtttc gtactcctgc
    20101 ccttttgtca gtcccgaccg ttccagaact cgctccagta ctacctcacc ccaattcccc
    20161 tgtttcttca catctccttt gagtgctttc gtcaggttgt tggcttcatc gctcacacgc
    20221 gagttcaact cggtgagttt ttttatttct tcccgcaaac ttattttatc acgcaaatcc
    20281 ttatcgtagg tttcttctac tttcttctca aaaagcataa ttttgtcctg taaggggttt
    20341 aaaatctggg acaaactttt atgatttgta actgagaatt catccgatcg ctcctttaga
    20401 atttttgtgg caatattctc gaattcagct gtcagacggc tttgtatctc catcagttcc
    20461 ttcttctgac tttccagttg ctgcttcagg ttactgttta ctgtttgaag cgcagcaatt
    20521 tccatgccgc gattttcggc aagtgcgctt atttgtacca gctgctcccc tactttcagc
    20581 aattcatcag tctttatatt caatctttca ttgctgacaa tatattgatt ctcaagagaa
    20641 ttcttttcat ttcggagttc tctcaggtta atttcaaata cttccttctg acggttgatt
    20701 tttcctgatt gaacgatcca ggcagacaaa aaacctgtaa tcagaataat aacagcaact
    20761 aaaataaata atgttacatg cataaaccga tagagttatt ttatattgtc aaataaagtt
    20821 ggctggtcag catccagttt acggagaatt tcagtcatca gggtttcacg aataaatttt
    20881 gatttgtttt ttactttata tttttcaata aatcggttta acgcattgtt ttccttatcg
    20941 ttcagggtga atatctgcct gtgttttcga agtaaaacag gatttggaga gggtttcagt
    21001 tctttcttct tggtcatttt ctgattaatt tccacaaaaa taatattata tcgttgaatt
    21061 tcagtattaa ttcccgaaaa agagtatttt tgcgacgata ctttttacaa atttcaactg
    21121 acttcaggct tgtatttgcc tgctgtttaa tcaattattc ataagttaaa aatggctgaa
    21181 cacaacgatc tgggaaatat cggcgaggaa cgagcacaag cttttctgcg caacagcggc
    21241 tacagaatca gagaaaccaa ctgggtagcc ggaaaactgg aacttgatat tgtggccgaa
    21301 aaggacgaaa tgctcgtcat cgtggaagtt aaaacacgct caaccgaatt tttcgaacat
    21361 ccgaaagaag cgattacacc gcgaaaaata aaaaatctgg taaatgcagc tcacgcatac
    21421 atcatgaaaa acaactggca gggcgaaacc cgctttgacg tcatatcggt tattccgaag
    21481 ggtcagaact tttccattga gcatatcgaa gatgcttttt tgccgccgat tatgtgaagt
    21541 taaaactcaa gatatcattt caattgctgt tgcaaaacat attcatttaa cttttaatta
    21601 ttaattagtt tatgtacttt tgcaaaatat aaacagttga atttgtgctg ctcgtgcagc
    21661 cttttccaat acccaattca agaaaaaaaa tgaaatcatc cgaaaaatcg atagctgaaa
    21721 aactattaaa agttaaagcc gtaaaattac aacccaacaa ccctttcatc tgggcttcgg
    21781 gatggaattc acccatatat tgcgacaacc gcaaaacact gtcatatccc gcaatccgca
    21841 actacatcaa agtagaactg atacgcacca ttattgactt gtatcccgac gtagaagtta
    21901 ttgcaggggt ggctacaggt gcgattgccc agggtgctat ggttgccgac gagcttggat
    21961 tgccatttgt atatgtacgc tccagcccca aagatcacgg attggaaaac cttattgaag
    22021 gcgatttgaa accaaaacaa aaagtggtaa ttatcgagga cctgatatcg acaggtggca
    22081 gcagcctgaa agccgtggaa gcaatccgta aagatggttc ggaagtattg ggaatggtgg
    22141 cgatattcac ttacggcttc gcactggccg atgagaaatt caaaaatgcg aaagtaaaac
    22201 taacagccct gagcaactac gatgccgtac ttactgaagc acttgccaca aactacatcg
    22261 ctgataaaga tgtggaaaca ctgagagaat ggagactgaa tccgtcagtg tggagaaaat
    22321 aatcaatttc acagaaaaat gactcttttt ataaaacaca ataaatgaca acatacgaaa
    22381 gtgacattaa aaccatttct tcttccggag aagtggtttt taacatatta agcaacctgt
    22441 caaatttcaa taatctccaa aatctgaatg ctgacgctca ggaaaaactg aaccagatgc
    22501 tgaaagacgt ggagtttgat gaaaattcat gccgttttac tgtcgatgga gtaggcaggg
    22561 ttggtttccg gataatcgaa cgcgagccgt tcaaaaccat aaaactggaa gccgaaaact
    22621 ctcctgtagc tgttaacggc tggattcaac tggtacaggt ttcggaaact gatacacgaa
    22681 tgaaactaac cctaaaagcc gacataccga cgatgattaa agtgatggtt gacagcaagt
    22741 tgaaaaaagg tataaatact attgcagata cgttagtcca gtatctgaca taccaaatcg
    22801 gaaaataaaa atactgaaac caatatcaga ttttttatca ataaattaaa attaaaaata
    22861 gtcccattca gggagaaata caggagagaa gaatatcatg aaactttggg agaaatcgat
    22921 acaggtaaat gaaaaaattg agaaattcac tgtcggtcat gaccgtgaaa tggatattta
    22981 tctggctaaa tatgatgttt tgggctctat ggctcatata cagatgctcg aatcggttgg
    23041 tttactctcc aaagaggaac tccccctact gctggctgag ctgaaaaata tttatcattt
    23101 gattgaaaat gatcaatttg agatagaaga gggcatagag gatgtacact cgcaggtaga
    23161 actgatgctt acccgcaaac tgggagatgt gggcaaaaaa atacacagcg gacgctcacg
    23221 caacgatcag gtccttgttg atctgaaact ttttgcccgt gctgaaatag aaaaaacagt
    23281 tgaagctgtg tcagggctat tcgatgtgct gactgaacaa agtgaaaaat acaaaaacat
    23341 tctgctcccg ggatacactc acctacagat tgccatgcca tcgtcattcg gactttggtt
    23401 tggcgcttac gcagagagct tggccgacga cctgcaaatt atgctttctg cctggaaaat
    23461 caccaacaga aatccgctcg gttcggcagc cggatatgga tcatcgtttc cgctcaaccg
    23521 tcagatgacc accgacttgc ttggtttcga caccatgaac tacaacgtgg tttatgccca
    23581 gatgggtcgc ggaaaaatgg aacgtatcgt tgcaagtgcc cttgccggcg tagctgctac
    23641 actttcaaag ctggcttttg acgcctgttt gttttcctcg caaaactttg gtttcatcaa
    23701 actacccgat gagtttacca ccggttcaag cattatgccg cacaagaaaa atcccgatgt
    23761 atttgaactc acccgtgcca aatgcaataa aatccagtcg ctgccacaac agataactct
    23821 gattaccaac aatctgactt ccggttattt ccgcgattta caaattatca aagaggtatt
    23881 cattcctgcc tttgccgaac taaacgactg tatggaaatg gtttcaatga tgctgcaacg
    23941 ggtggaagtg aaacaaaaca ttctggatga cccgaagtac gattatctgt tcagtgtcga
    24001 agaggttaac cggcttgccg ctgaaggaat gcctttccgc gatgcataca aaaaggtcgg
    24061 acttgccatt gaggccggca atttcaagcc cgtgaaagac atccgtcaca cgcatgaagg
    24121 cagcatcgga aatctgtgca atgaccgtat aaaaatatac atggacagta tcagggaaca
    24181 attcaacttc gggacggtac acgaagctgt aaaaaatctg ctggaaagtt aagacttacc
    24241 gggtataacg acgccgccgg gtccactgaa ccaatattcc gaaaataata agtatcaccg
    24301 gaggaattac gacattaata atctgccatt tagttttctg actggcagat atttttttgt
    24361 tcagtaaacg caaagcaaca gttttcgagc gcagttccat ccatccgctc tgatcagtca
    24421 gataaagtac agcgttggtt atcagctcat tattagcgaa ttgctggttc atatagcgat
    24481 cataaccgag tggtacagct ttcaggtttt caccagttcc ttctgtttca ttgcgtataa
    24541 tatcaccatc ggcgatgatg atctgtcggg tgcgaacact tttattctga ataaccgaag
    24601 ggttgacaat ttctttggga gccattctgt ttgaaaaatc agactgaaat tcaccttcca
    24661 gtactactcc taccggcaca taagcattat tgaaatattt tttatccttc agatcaggca
    24721 tctgcaaaag cgaaatttca gcaggcgttt gcagaatatg tgtattgtct gaactggcaa
    24781 ggatgaaatt tcgttccagt tttttatcat cacccacaat ctcgatggcc gaagcaaaat
    24841 tcgctttgac aggagccaga tttcgggtaa ccggatgttc gcccgaagta agcaacaatg
    24901 gcgcgttgac ccagggcatt ggctgaaagt caggttgctg tcctttggca gccacattga
    24961 cgggaacaag tacactctgt acatcctgaa ccagcacggg actgatgcgg acaccataac
    25021 ggaaaagtat gtcgtttaga ttcaagtcca gttcaatcgc cggagatatc cctgcagtcg
    25081 taagactttc ttccgaaaca cgcaccccat ctatcagcca taataccctg cctccgtgca
    25141 tgacgtactg gtcaatcaca aatttatctt tttcgctgaa aggtcccatc ggtttggcta
    25201 tgatcagagc tttataatcg ttcaatatgt taggatctgt atcaataatt ccgcggtcaa
    25261 tctggaaata gcggctcagg ctccggctga tatcataggt ttcggtttcg ttaagttctc
    25321 cgtgtccttc cagaaatgca attttttcca ctttcgtatt cacgagcctg cgaatggcgt
    25381 cagtcagttc gaattcaaga ttttcaacag aaatattcag attttcttca cccgaaattc
    25441 cacggatatt tttcagcagg tttacgggaa ttgttttccc cttatagcta atttccagcc
    25501 acggaaaaat tattttctga atattctttc cttccttatc cttatcataa accgaagttg
    25561 ctgtcatgcc cttctgctcc agttcggcat atttctgttc acgttcctca ttcgtttccg
    25621 cctctgatgg attgacataa tttatgctga tattcccttc ggcataagcg ctcaactcat
    25681 cgagcaattc cgcaaccgac tttttcagtc tcagaaagcc cggattgaga tctccggtga
    25741 ggtaaattgt cacagtaaca ggttcgcgta ccgatttcat gagtgttttg gtttgtttac
    25801 tgatactgta acgcttgtcg gaagtgaggt cgatgcgata aaagaaaaat gaagagacaa
    25861 ccgtcagggc tatcaccgta aaaagaatta tgtatgtcga ttttgatatc tttttcattc
    25921 tcagaatatt ttaatccggc agattttgcg cccgaatgat aaaataattg ttggtagtgg
    25981 aaagcagacg atattcagca cgatttcccc acgatgcagg catatccttt atactcatat
    26041 cctcaccggt atcgttgttg acaaaaggag atatgagggt atgccatttt ttaatcctgt
    26101 ctatgctttt agtataatag aataaatcat tatccggatt gcaaagttca tgaaaataac
    26161 tcacatataa agctctgtac tcgggaatat ttataatttt tttcaccaaa ggatttgaat
    26221 cggagcccca gcggagtaaa tcctgcgttc ccgaattggt catgagcaga gatgtcccca
    26281 gcgtattgtc ataatcatac ggaataaaat aaaacttatt gtcggtatcg aaatagaaat
    26341 agaaattatt actgttattc cagtaatcgt cccacatacc acagagagta cttaccgcgt
    26401 aagttttcag aaaaagcggc acgtccatcc gttgttcaat ccatgttttg aaatcatctc
    26461 ccgttttact gttgaaatcg gtgataaatg cagccaactg tgttttggca gcttccagat
    26521 attcttcatt ggtcttcagg tcgtacacag gctgaaccat gtcggctttg gtgagggaaa
    26581 taatttcaac tcccatcctt cctttgtctg catttctgag atcggcaccc caggtagcct
    26641 tccataaaaa tccgtcttta ccggcaaacc taccggaacg gtttttcaga tattgcttat
    26701 ctatctgttc cacaacctga taaataccaa aataaacctg cttgttatct tccttaattc
    26761 gcaaatacaa cttacaatag cttgatttcg gactcgtcca gaccccaaaa cgttcgaata
    26821 aatcatagca ataaacttca cgggcataaa cgccatcatc tttgaaccat tttagtgtga
    26881 ttttttcaag tccgtgcaaa tcctgctttt tgttgtactc atcaaaatta agtgtgaaat
    26941 gcacatgatg ccagtcggga tttatactgc tgtgcatttg cccggtatta ccttcgggtc
    27001 ttctgcggct ggtatttcca cgaagccgta aacccgtgtt aatcaaggta tctcttcttg
    27061 acgcctttac aaacacgcaa ttactctgga tatattcttc gttatcctta ttaatatcaa
    27121 aattggagag taacctgttc cattcggcag tggtaatttc cagataaact tccgaaacag
    27181 cttcaatatc gaacacatac tgtaaaccgt ctgaatgact gttaatctca tcctgcggat
    27241 tatgaatttt gggtggatcg gtcgtgaccc cggaaaagtc ttctcggcag gaggtaaacg
    27301 cgagtaaccc aaatataatc agatatataa actttttcaa tttaattaaa tcatatatta
    27361 ttgcaaagat atacaaattc ggagtataaa gctatggagc aaaactgaaa ttgcaagcat
    27421 tcagtaaact acctgtataa aaaaatctgc cgtcatatcg aaaaaatcgt tcaatgatgg
    27481 taacagaatt ttaaaaaatc ttataatgtc gattgaactt tttgatagtt taacggctga
    27541 attaatttta ttatctttgc agttaaaaca gcaaataaca atatatgctt tccactcgat
    27601 tatagaatgt tcaaagagca atattgtcag aacttattaa gagtataaaa ataatttcga
    27661 actaatatat gggtaaaata atctcaattg ccaatcaaaa aggaggagta ggaaaaacaa
    27721 cgacagctat caatctggct gcatcgctgg caggacttga caaaaaagtg ttgttggttg
    27781 acgcagatcc gcaggcaaat gcatcatccg gactgggaat tgatgttcga tcggtcgaat
    27841 tcactgttta tgaatgcctg attgacaacg tgccaacatc acaggcaata cacaaaaccg
    27901 aagttaacaa cttgtttgtt ctcccttcac atattgattt ggttggtgca gaaatcgaac
    27961 tgctgaatct cgacaagaga gagcatatca tgcagcgtat gcttatgcct ataaaaaatg
    28021 agtatgacat tattctgatt gattgctcgc catctctggg attgataact gtcaatgcgc
    28081 tgagcgcctc cgactccgtt atcattccgg ttcaaagcga atacttcgcg ctggaaggca
    28141 tcagcaagct gctgaataca attaagatta taaaaagcaa attaaatccc gcactcgaaa
    28201 ttgaaggttt cctgatgacg atgtacgaca acagattacg ctctgccaat caggtatttg
    28261 ccgaagtgaa aaagcatttc gacacgctgg ttttcgacac ggtgatttac aggagtgttc
    28321 gcctgagcga ggctccgagt tatggcaaac caatctcgct ttttgatgca gaatcacgtg
    28381 gtgccataaa ttacatggac ctggcaaagg aattaatcaa cagacaatag aaaaatttta
    28441 cacattcaag aattaattat ggcaaaaaac acaggactgg gacgaggtct gggtgcattg
    28501 attgacatgg attccgtgaa cacaagtggt tcgtcatcca taaatgaagt tgatatcgaa
    28561 ctcatctatc caaatcccaa tcaaccaaga acatatttcg atgaagaggc gctgactgat
    28621 ctggccgtat ccattcgtga actcggtgta atatcgcccg taacattacg taaaaatgat
    28681 gatggcactt atcttatcat tgccggcgaa cgacgtttca gagctgcaaa gtcggtagga
    28741 cttaacacaa ttcctgcgta tgtaaaaaca gctgctgatg aacaggtgat ggagatggca
    28801 ctgattgaaa acatacagcg ggaagatctc aatgccatcg aaattgcgct gacattttac
    28861 cggctgatgg aagaatacaa actcacacaa gaccggttga gcgaacgggt gggcaaaaaa
    28921 agagcaacaa ttgccaacta tttgcgttta ttgcgtctgc ctgcggaaat tcagatgggt
    28981 ttgaagaata aaaaaatcga tatgggacat gcacgtgcca tactgggatt gaacgatccg
    29041 gccgaacagc taaaactcta cgaacagatt ttaataaaag atctgacagt aagaaaggtg
    29101 gaagagctcg ttcgaagcat tactgaaacc ggaaaacccg aaaatacttc agataaaaac
    29161 aacagtgtta aggatctgaa agaattaaac gacctgaaaa agcagctgag caccattttc
    29221 gatgcaaaaa tacagatgtc ttgtgacgcc aaagggaaag gtaaaatcac tatcccattt
    29281 gcaaacgatg acgagctgat gcgcatcatg caactgatgg ataaaatctg aaaaacaaac
    29341 aatgaagttg agaaaaacat ttgtcatata tttttttctt cagttcattt ccctggcggg
    29401 ttttgccggc aataatgact ttcaggagaa cagcattttg aaccttgccg gaaccggaca
    29461 ggatacaact acgttaatgc caataaatat tcagcccaac agtaaataca tgaatatggc
    29521 aaatgatatt gctgatacgc tggctgtatc ttcgcttgaa aaaacgaaat tcaaaccgaa
    29581 ccctctgaaa tctatctgga tgggtgcagc tatccccggt ttcggacaaa ttgtaaacag
    29641 aaaatactgg aaactgccta ttgtatatgg aggatatctc ggatttgctt atgccatatc
    29701 gtggaataat aataattata ttacctacaa aaatgcttac cgggatatta tcgacaaaga
    29761 cccgactaca aacagccata tcgatattct tccccgcggt tacaccattc agatgattgg
    29821 cgaaagcact tatactaaca tgctgaaaac cagacagagt acatttcgtc agtggcgtga
    29881 tctgagcata atactttctg taggatatta tgctctggta ttgctggatg cttatgtcga
    29941 tgcacagctt tatgactttg atataagtcc cgatttggtt ctcaatgttc agcctacccg
    30001 tatcgaacgg gaacgcaatt cgcctgcagc ttacggattg cattgcagca ttaattttta
    30061 acggttatta cgctgattcc acagctgttt tttcagtata tccttttttt atttattttt
    30121 gtttttttat attgattccc ataaacgctt ttcgtcaaaa gtgctaagaa tagcctgcat
    30181 gttactctaa ttaccaaact gactgttcat gaagaaaaga atttttatct acttactttt
    30241 ttttctgtca attgtttttc agactcacgc acagagttcc ggtgcaaagc cggtggttac
    30301 agatattgca accgaagtaa catcagattc gttgattgtt ccggccgaat tttcaggttc
    30361 tctcgacctt ctgcttcatt cgtgggctgt tgacaaagac aatgtgtccg aatgttcatc
    30421 cagtcccaat ccttcaacct ccgacgagca atacaaggcc agattaatga agttacctca
    30481 tgtaatagaa atgcctttta atcaggaagt aaagtcgttt atcgaaatat acacccagaa
    30541 aaaccggaaa ggacttgaat acatgctggg attaagcagg tactacttcc ccattttcga
    30601 agaagcgctt gaaaaagaac agcttccact cgaactgaaa tacctgccaa tcatcgaatc
    30661 tgcactcaat ccggtggctg tatcccgttc tggcgctacc ggtctatggc aatttatgat
    30721 tggtacagcc aaaatgtacg ggctggaagt caatacactt gttgacgaac ggatggaccc
    30781 gcgcgcctca tccaaagcag cagttaaata tctgaaagaa ctttactcga tttattccga
    30841 ctggcatctt gttattgcag cttataattg cggaccggca agcattaata aagccattcg
    30901 ccgggccggt ggcaaacacg atttctggac aatatattcc tttcttccgg cacaaactcg
    30961 tgcttatgta cccattttta tagcagccaa ctatgcacta aattatgcaa atgagcacaa
    31021 tctttgccct gcacaaacaa gaattccggt tctcacagat actatattgg tcaattccag
    31081 aatgcatttt gagcagatct cggctgtact caatctttcg gtggaagaac tccgactgtt
    31141 aaatccacaa tacagacgca atataattcc gggtgatatc cagccttacc cgctatgtct
    31201 tccccaaaag tactcaggta tgctaattga aaaaaaggat gaaattctcg cattcagagc
    31261 tgatgaactg attaataacc gccgcgctga aatcataaca tcagaaaaag aaactgaaat
    31321 caaagctcct ataaaaagaa aagaacagtt tacttactat aaggtcaaga aaggacagac
    31381 acttggcggc attgccgaaa aatttgacat cagcgtatcc aatctgcgaa aatggaatag
    31441 catatcgggc agtaagataa agccgggtca gcgtctgaaa attcgtcaaa gttaaaataa
    31501 tatacaggga atcatggaaa aaatttatta ctctatatct gaagtcgcca cgatgttcag
    31561 catcaatcag tcaaaccttc gtttttggga aagggaattc aagcaactga aaccaagacg
    31621 caacgaaaaa ggaacgcgtt tttattcgaa agatgacatt caacttatca aacagatcat
    31681 ttttctgatc aacgaccaga aactcaccct ggatggcgta aaaaagaaac tgagtgataa
    31741 aaaggatgtt gttgtcaaac aacaggaaat taaggaaaga ctaacagcta tcagagatga
    31801 aatcaaaggg atgattgctt taattgacaa taacaaataa accttcagag ttacacagaa
    31861 tgtcctccat cagatacgag aaagagacgg tagaaaagat gatcaggctg tattgccgca
    31921 aaaaacacaa atccgaaaaa gaattatgtc ctgaatgtgc caaagtaagc aactacgctt
    31981 ctgcaagact cgacaactgt ccttttggtg atgaaaaaac tgcctgctcc gaatgtaaaa
    32041 ttcattgtta taaacctgag atgcggcaaa gaataaggga aattatgcgg tattcgggtc
    32101 cccggatgct gctttaccat cctttcgatt ttgtacagca tatgctcaag gacaaaaaat
    32161 aaagtaagct gattcaaaga actgaatcag cttacaacat actataaaac cctttttaat
    32221 tatttgtttg cgggcgcagc aggcgcaaca actgccggct gatctttcac tcctaatttt
    32281 ttcttaacca acggagtaat atcgttggct gttttggact gaaaaacaac accctgtgtt
    32341 gaaatatcga agatataagt ataacctccc tcagtagcta cgtcactgat agcctttttt
    32401 actttttcaa gtaccggatt taccagttcc tgttgttttt taacaacgtc agcattcgct
    32461 gtctggttga atgtagtgat acgttgttcg atttcggcta tttctgactg acgtgctgtt
    32521 tttatagttt caggcattgt agcttgctta tcaacgaatt cttttacctt ggtattatat
    32581 tcttcacgca ttttcagcaa ttccttttcc cattgatcct gaagttcctt cagcgttttt
    32641 tcaattgttt cgagttcagg catcacctga ataatctcct gagaatttac gtgtccgagt
    32701 ttaacatttt gtgccatagc tcccagcggt aacaaggcaa tcaagaacaa agcaactttt
    32761 ttaatcattt tcttttctat taaattaatt tctagtgatt tatatttatt gtgagaatac
    32821 aaaagtatat aattattttg aataacccaa ttttttcaaa acatcatcgc tgatgtcaag
    32881 cttaggcgaa acgaaaatag cactcatcgc tgaactcttg tcaaatacca actgcaggtc
    32941 tttatcctta cttatctcct gaatagcagt ataaatctcg tcctgaattg gtttcataag
    33001 actttcacgt tttttgaaca attcgccttc aggtccaaag tacttgcgtt ttagttcctg
    33061 agcttccttt tctttagcca caatttcgtc ttcccgcttc actttcatat cgtccgacat
    33121 gaatacaagc tctgtctgat agttcttata catctgctga acatctttca tagctgtttc
    33181 aacttctgtc tgccatttct tggaaacctg attgagttgt tcattagcac tttcgtaagc
    33241 cggaatgttt ttcatgatgt acgacatatc gactgcagcg aatttctgag cctggacacc
    33301 gattgcaagt cctgccagaa ggctaagtgt aataaataat tttttcatcg tttagagtgt
    33361 tttatcagtt tgatattttt atcgttgtaa gtaatatatc aaaatctttg ccaaactttt
    33421 acatgttgaa tatctgatac ttacaagaac cgattaattt ctgattacaa ttcctgacca
    33481 atcacaaagt ggaactggct tccgtgtgct tttgattcgt taa
//
LOCUS       DAOPYB010000015        32938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1255_length_32938_cov_8.888848, whole genome shotgun sequence.
ACCESSION   DAOPYB010000015 DAOPYB010000000
VERSION     DAOPYB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 32938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32938
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1255_length_32938_cov_8.888848"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKH58_02335"
     CDS             <1..300
                     /locus_tag="PKH58_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNX87896.1"
                     /translation="CNQFGNQDPGTDEEIQSFCQLNYGVTFPMFSKIDVNGDNAHPIF
                     SYLKNSLSGTLTDDIKWNFTKFLIDPTGKPLKRYGSNVKPESIEADIIKLLNPSK"
     gene            531..1808
                     /gene="metK"
                     /locus_tag="PKH58_02340"
     CDS             531..1808
                     /gene="metK"
                     /locus_tag="PKH58_02340"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNX87897.1"
                     /translation="MGYLFSSESVSEGHPDKVADQISDAIVDNFLAQDANSKVACETL
                     VTTGQVVLAGEVHTQAYVDVQSVARGVINRIGYTKSEYMFDGNSCGVLSSLHEQSPDI
                     NRGVEREDPMNQGAGDQGMMFGYACNETVNYIPVALDLSHALVKELAYIRREGKVMTY
                     LRPDAKSQVTIEYNDDNQPERVHTIVVSTQHDEFVKPSEGKTQQQADEEMLAVIYNDV
                     KNILIPRVKAKVIQFDQLIKDDYILYVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKG
                     AHGGGAFSGKDPSKVDRSAAYAARHIAKNLVAAGVSDEVLVQVAYAIGVAKPMNIYIN
                     TYGKSKVNLTDGQIAQKVEKLFDMRPKAIEQRLKLRTPMYEETASYGHVGRTPIIKEK
                     TFGYNGNMKTVKVELFTWEKLDMVDEIKKEFGL"
     gene            1988..4408
                     /locus_tag="PKH58_02345"
     CDS             1988..4408
                     /locus_tag="PKH58_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNX87898.1"
                     /translation="MNRDEIYSQINRIYGFLKVKLFQFINWREKRIIRFYGLPKWRKV
                     SNTILTFFGLFLFYLILVDINFLWLFGKSPSLRAISNPPQSLSSEIISADGKLIGKYF
                     NENRKLVKFEDISPSLINTLISTEDHRFYKHFGVDIRGLFGAMKDMIQGNPRGASTIT
                     QQLVKNMYKTRSQYSRGLLGSLPGLRLPLEKTKEWTAALKLEIFYSKNDILEMYLNTV
                     SFGSNAYGIYTASKTYFNTTPDKLNYEQSAVLVGLLKAITKYNPYINPKNSLKRRNVV
                     LNLLVHNKYITQQAADSLKEIPIQLNYNIEKPNQGIAPYFRANLTRFLEKWGETNGYD
                     IYADGLKIYVTIDSRMQQYAEDAVRKQMKQLQRNFFAHWSGQNPWRDENGNELKNFIS
                     DIAKHTRVYRNLASKYNNNQDSIDKYMNLPRKMKVFDYDKGVKTFTMSPMDSIRYVNH
                     FLHCGFVAMEPDTKYVKAWVGDVDYDNWQYDKVAQSKRQPGSTFKLFVYTAAMIAGKS
                     PCDKITDKPITWGTGEDAWSPKNSTGGFSYEDITLKSAFAKSVNTVAAQLAKEVGLHN
                     VAKCAHLLGIKSKLDEVPTLSLGASDVSLLEMVNAYSTVTGEGMYHSPIIVTKIVDKD
                     GYTIYEEKIKPKRVLSYENAFLMTELLKGGITEPGGTSRALWNFDLMRWDTEFGGKTG
                     TSSNYSDAWYIGVTPKLVGGAWVGAEHRSVHFRNGSMGQGSRTALPVFALFMEKVLSD
                     KQLSHYRAKFPAEPKQPISRDYKCQSYVKPDTALVDSTIVEPIETLPVPDLDLSKPDE
                     TTNPVTPQ"
     gene            4467..5294
                     /locus_tag="PKH58_02350"
     CDS             4467..5294
                     /locus_tag="PKH58_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNX87899.1"
                     /translation="MNKIISKEYFSANVVKFVVEAPLIAKARRAGHFVIVRVGEKGER
                     IPLTIADADISKGTITLVIQRMGVSSTKLCALEAGDYITDLVGPLGKATHIENFGTVV
                     CACGGVGTAPMMPIVAALKKAGNKVITVLAARTKELIILEEQMREYSDEVIIMTDDGS
                     YGNKGLVTNGIEEVIQREKVDMCVTIGPAIMMKFVSILTKKYEIPTVASLNTIMVDGT
                     GMCGACRVTIGGKTKFVCVDGPEFDAHQVDFDEMLMRLGGYKDQEKEEMEHKILHTK"
     gene            5300..6760
                     /gene="gltA"
                     /locus_tag="PKH58_02355"
     CDS             5300..6760
                     /gene="gltA"
                     /locus_tag="PKH58_02355"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNX87900.1"
                     /translation="MNTKRDEQWRVDLRAKIKAKDRTNLERVHMPELDPEYRSHIRNE
                     EVNLGLTPEQAMAEAQRCLDCANPTCMEGCPVGIQIPDFIKHIETGNFLEAAKVLKLT
                     SALPAVCGRVCPQERQCEEKCIHLKMKKDAVAIGYLERFAADYERESGQISVPETAPA
                     NGIKIAVVGSGPAGLAFAGDMAKYGYEVHVFEALHEIGGVLKYGIPEFRLPNKIVDVE
                     IENLKAMGVIFTNNCIIGKTIAIQELEEKEYQGIFVASGAGLPRFMNIKGENLVGIMS
                     SNEYLTRVNLMDAANPDSDTPVYKGKKVAVIGGGNTAMDSVRTARRLGAEKAMIVYRR
                     SEEEMPARVEEVKHAKEEGIEFLTLHNPLEYIGDANGRVTQMILQEMELGEPDESGRR
                     SPVEIPGKTKTIEVDEVIVSVGVSPNPLIKQSVPELETTKWGTLVVNQDTMQSVSIPI
                     IFAGGDIVRGGATVILAMGDGRRAAKAMDEWIKSKG"
     gene            complement(6836..8065)
                     /locus_tag="PKH58_02360"
     CDS             complement(6836..8065)
                     /locus_tag="PKH58_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87901.1"
                     /translation="MKYNNYTFALIIFLIISSFLFTGKAQTAPKYELYGFVRNDFYYN
                     SRQNVEVIDGIFNIFPKPLVHDADGNDINNTAQAEMISVASRLGLNINGPELFKARST
                     AKIELDFCGTGTTYFLVRLRQAYMKLNWKNTELLAGQTWHPLYGNVAPTIVSLNSGSP
                     FQPFNRSPQLRVKQNLTSSLSVSLAGIYQMQYTSYGPEGKSNNYMKQALLPDMYLGME
                     STGKHFTGGIGLDYKTLKINKSRLGSKSAMIYGQYASKKLQIKAKSLIGQNLSDLQMP
                     MGYGLSSYNVENKLHTYTNFNQSASWINLVYGKTWQVGILGGYLKNLGTDKNLLPTAN
                     NDYTVYSSGYFSGNQHLDQLIRIAPHLSYNMKNIRLGLEYDLTNAGYGKLNTQGKVTD
                     PYSVTNHRVVGVAVYNF"
     gene            complement(8104..8670)
                     /locus_tag="PKH58_02365"
     CDS             complement(8104..8670)
                     /locus_tag="PKH58_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNX87902.1"
                     /translation="MDFLSILLISIGLAMDCFAVSISKGICVGKFSFKNTLKMAFLFG
                     FFQGIMPLIGFYAGKSFIHELSSFDHWVAFGLLGIIGGKMLIEGLKPIDPDCEITPDP
                     FKLRLLISLAFATSIDALATGIIFIPFPDIILSAVLIIGIVSFLFTFLGMGIGIRFGK
                     RFKFNVEILGGIILIGIGTKILLEHLFL"
     gene            complement(8749..10770)
                     /locus_tag="PKH58_02370"
     CDS             complement(8749..10770)
                     /locus_tag="PKH58_02370"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNX87903.1"
                     /translation="MHTTQDLNRAADNIRILSAATVEKAKSGHPGGAMGGADFINVLY
                     SEFLEYDPENPCWEGRDRFFLDPGHMSAMLYSVLALTGKFSMEDLQQFRQWGSITPGH
                     PEYDLSRGIENTSGPLGQGHTYAVGAAIAAKFLKARFGEVMNQTIYAYISDGAVQEEI
                     SQGAGRIAGHLGLDNLIMFYDSNNVQLSTYTNSVTSEDVAMKYRAWGWNVIEINGNDV
                     TEVRVALREAKAEKEKPTIIIGKTIMAKGAVASDGRSFENEIETHGQPLGASGASFVD
                     SVKNLGGDPENPFVIFEETKAIYAKREVQLKEIMAEKKQAAKAWEAKNPELAAKLAQF
                     FSGNAPEINWDEIEQKPNQATRAASATVLSYLAQHVENMIVSSADLSNSDKTDGFLKK
                     TKAFAKGDFTGKFLQAGVSELTMACLCNGMALHGGVIAACATFFVFSDYMKPAVRMAA
                     LMQLPVKYIWSHDAFRVGEDGPTHEPVEQEAQLRLMEKLKNHHGQNSMLVLRPADVEE
                     TTVAWKMALDNTKTPSALILSRQNITDLPAANRKEEAKKAEKGAYIVESDENPDVILL
                     ASGSEVATLVEGAALLRADGIKLSLVSVPSEGLFRSQSEEYQETVLPKNVKKFGLTAG
                     LPVNLLGLVGDNGKIWGLESFGYSAPYKILDEKLGFTAENVYKQVKSLL"
     gene            complement(10800..11525)
                     /locus_tag="PKH58_02375"
     CDS             complement(10800..11525)
                     /locus_tag="PKH58_02375"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HNX87904.1"
                     /translation="MSKTLKYILLSSVILFAGLAQAQQPQNNSLANAKTTIQSKKDTT
                     TIPVWQGINIEIDIEPFIENAFTNNSTRNVYSYQGNVQANLKNSYLPVVEIGMGGATR
                     NLTNNTTFRGEGMFGKLGVDFNLLKPKPGTKINNNYFLAGARIGMSQFNYTTDNLTIE
                     DNYWGGTETINYSMPATKIWFEIVAGVRVEFFKNLYLGWNVRNKHLLNNGKYGAVTPW
                     YIPGYGRGETSAWGFSYTVGYHF"
     gene            complement(11491..11982)
                     /locus_tag="PKH58_02380"
     CDS             complement(11491..11982)
                     /locus_tag="PKH58_02380"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HNX87905.1"
                     /translation="MKKLYFWLLPVLLLTSGLISCNNDETCRKSKTVLMGAKFYQDTL
                     NLKTNQYVTINFKADSLTASGVNNDSIIYKKVTNLSEIKLPVDKLKTQSRFSIKFNEV
                     YDTITVLYHNTDEYLSLECGCVRTHTIDTVLTTNHIIEKIKIIQPIINTTYVENIKIY
                     LAE"
     gene            complement(11991..12506)
                     /locus_tag="PKH58_02385"
     CDS             complement(11991..12506)
                     /locus_tag="PKH58_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87906.1"
                     /translation="MWIEILKYTIPAVLVVLVTYLLLNLLFKNEAERRNFELKKANNT
                     TVTPIRLKAYERLMLLMERIHPNRVIVNKVEAGMNCFELQQVLLNDLRREFEHNLSQQ
                     IYVSSELWESIKDAYENVIQLINTCASQCQAEESAAKLATMIIKLYDSVDETALDVAM
                     QVLKDEVKEII"
     gene            complement(12512..12862)
                     /locus_tag="PKH58_02390"
     CDS             complement(12512..12862)
                     /locus_tag="PKH58_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNX87907.1"
                     /translation="MKSENSILKIYVSNTDKFGMSLFYEHLVMKAYKKGISGATAYRG
                     ITGYGLSGNKIVSGKFWEINEKLPVIIEMIDRTEVLENFIEDIKSDIEAFGKGCLVYM
                     QPIDIIIHQSGKKK"
     gene            complement(12865..13239)
                     /gene="crcB"
                     /locus_tag="PKH58_02395"
     CDS             complement(12865..13239)
                     /gene="crcB"
                     /locus_tag="PKH58_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNX87908.1"
                     /translation="MKQLLLVGLGGFIGSIARYLVSKLNLTWYFHDIPMGTLTVNILG
                     SILIGFFLGIFVNSDLLNTNLKLFLVVGICGGFTTFSSFTNENFQLLQNGQFLTAAVY
                     ALGSLLFGVIAVYLGYLISNLL"
     gene            complement(13297..14247)
                     /locus_tag="PKH58_02400"
     CDS             complement(13297..14247)
                     /locus_tag="PKH58_02400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX87909.1"
                     /translation="MDISVIVPLYNEEDSLPKLYEWVDKVMTDNKFSYEIIFINDGST
                     DGSWRIIEQLKSQSPHVKGIKFRHNYGKSPALYCGFRAAEGDVVITMDADLQDSPDEI
                     PELYSMITKDDYDLVSGWKKKRYDAKLTKNLPSKLYNATARRVTGLKLHDMNCGLKAY
                     RRDVVKNIEVYGEMHRYIPYLAKNAGFTKIGEKVVEHRKREFGSSKFGMNRFVNGYLD
                     LITLWFLSKFGKQPMHFFGLIGSLMFILGFFAVIVVGVKKLIALSAHIPSPLVTNSPY
                     FYIALTAMILGTLLFLTGFLGELITRNAPERNNYKIEKEV"
     gene            complement(14286..14813)
                     /locus_tag="PKH58_02405"
     CDS             complement(14286..14813)
                     /locus_tag="PKH58_02405"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNX87910.1"
                     /translation="MQTNVLNAAMKYGLIMGIVFSVNFLLSVSGNAIITLLGYLVVAF
                     IFVLTYRFAVAYRDKENGGSISFGHAFFFIILLFLFAAIISSLFKFIYFKYLNVNYLP
                     MLLEQSVSMMQQLQEQFHLSVPDDYYETMDKMMSPTAFTLQYIWVNTFLGVIMGLIIA
                     PFVKKDKDIFNNPEN"
     gene            complement(15042..15686)
                     /gene="nth"
                     /locus_tag="PKH58_02410"
     CDS             complement(15042..15686)
                     /gene="nth"
                     /locus_tag="PKH58_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNX87911.1"
                     /translation="MTKQQRFQNIIAWFEENRPIAETELDYKDPFGLLVAVILSAQCT
                     DKRVNMVTPKLLYDYPTAEAMAASTPEVIFEYIRSVSYPNNKAKHLIGMSKKLVSNFN
                     GIVPDDVEKLQTLPGVGRKTANVIASVVFNKPAMAVDTHVFRISERIGLTTNSKSPLE
                     TERELVKYIPDHLIPKAHHWLILHGRYVCIARKPKCEECGIKSWCRHFEKNNKL"
     gene            15813..18260
                     /locus_tag="PKH58_02415"
     CDS             15813..18260
                     /locus_tag="PKH58_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNX87912.1"
                     /translation="MPPKKPVKKNTAEKPQLTRTKQRTSEPGLFSKALHFLRDDKLHF
                     IFGLLLSIAVLYAFLSFVSYFFTGAADKSIFDNISFRESLRIRGSVTNWTSVMGAYIS
                     ETLIDNWFGVSSAAILIFLLAVALRLMNVKIISIWKAFFHCFFWLVWISVSLGFITDF
                     IPSLQPSFFALGGQHGTYTAVQLNSFVGRPGALMIIAGALLIYLIVVWSATIPYIQNL
                     FNKNDNIQSERKHQEPIVAVGTEIVPEEWLVSEVSNSKETYTEKESSSLIENEPIDLN
                     EVVQFEVTNPVKDETNPVEEYEDEENTDPNYNVSKLGKYDPTLDLSSYVSPTLNLLKV
                     YGSENDMQIDMKEQTANKNRIISTLRNFGIEIDSIKATVGPTITLYEIVPKSGIRISK
                     IRNLESDIMLSLAATGIRIIAPIPGKGTIGIEVPNNEPQIVSMNSVIGSKRFTEAKFE
                     LPIAFGKTITNEVFMVDLTKMPHLLVAGATGQGKSVGLNAIITSLLYKKHPSQLKFVL
                     IDPKKVEFNIYSAIEKHFLAKLPDGEDAIITDTSKVVQTLNSLCVEMDDRYDLLKKAH
                     VRNIKEYNEKFVNRELNPEKGHRFLPYIVVIVDEFGDLIMTAGKEVEMPIARIAQLAR
                     AVGIHMIIATQRPSVNIITGVIKANFPARVAFRVSSMIDSRTILDAPGANQLVGRGDM
                     LFSQGSDLIRVQCAFVDTPEVEQIALYIGNQRGYPTAFYLPEYVGPESEGVDTSTVDM
                     SKRDPMFEEAARLIVASQQGSTSMIQRKFSIGYNRAGRIADQLEVAGIIGPNEGSKAR
                     QVLVQTEYELEQILKHL"
     gene            18387..19031
                     /locus_tag="PKH58_02420"
     CDS             18387..19031
                     /locus_tag="PKH58_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNX87913.1"
                     /translation="MNIKKIVFSLMIAIVAFTSIKAQSNSGAVKVVDNMLQLLKNNAI
                     KTNFEMVVKEPSSPQSHRMKGNFTMKENKFILNTAEMNVYFDGKTQSAYMTSVNEVSI
                     TNPTEKELAETNPMAIISSYKRKSTIKFVRSSISKSFHNIELIPKDKNSSFKKIVLNV
                     NKTNNYPLFIQLIDKGGMITDLTLTQFKSGLNISDNAFVFNPKKYKDIEINDLR"
     gene            19154..20095
                     /gene="trxB"
                     /locus_tag="PKH58_02425"
     CDS             19154..20095
                     /gene="trxB"
                     /locus_tag="PKH58_02425"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNX87914.1"
                     /translation="MNEKVRCLIIGSGPAGYTAAIYASRANLSPVLYEGMQPGGQLTT
                     TTEVENFPGYPEGITGPELMEDLKKQATRFGADIRFGMVTATDLSSAPYKITIDGEKV
                     IEAQTLIISTGATAKYLGLPDETKYAGSGVSACATCDGFFYRKRKVAVVGGGDTAAEE
                     AVYLANIAEKVYLIVRKDYLRASKIMQKRVMENPKIEVLFEHETLGLTGDMVVQGADL
                     IKRRGNPDEEKVHIEIDGFFLAIGHTPNSQIFKPWVETDETGYIKTIPGTPKTNVEGV
                     FAAGDVADPHYKQAITAAGTGCQAAIEVERYLSEKGL"
     gene            complement(20157..20408)
                     /locus_tag="PKH58_02430"
     CDS             complement(20157..20408)
                     /locus_tag="PKH58_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNX87915.1"
                     /translation="MTKTITFNELRRIKDALPSGSMAKIAEELGLEKQTVKNYFGSGD
                     YKEGKSVGLHIEPGPDGGIVQLDDTSILEVALRMLEEIK"
     gene            complement(20565..21929)
                     /locus_tag="PKH58_02435"
     CDS             complement(20565..21929)
                     /locus_tag="PKH58_02435"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNX87916.1"
                     /translation="MNIKNYIRDNQSRFLEELFSLIRISSVSSHTSHQPDIQQCAERW
                     KHLLEEAGVDYAEILPTTGNPIVFASKMIDPELPTIMVYGHYDVMPAEPLELWTSPPF
                     EPEIRDEKIYARGADDDKGQSFMHAKAFEYLVKTNQLKSNVKFLLEGEEEIGSPSLEE
                     FCSANTEKLKCDTILVSDTSMLSASTPSITTGLRGLAYWEIEVTAANRDLHSGIFGGA
                     VANPILELTKILAQLKDEEGHIAIPHFYDDVEDVSEKERELIARIPFSEEEYKANLGI
                     KQVFGEVGYSTIERTGIRPALDICGIWGGYTGEGSKTVLPSKAFAKLSARLVPHQKYE
                     KIAKLVEKHILQIAPEYIDVKVRQLHGAESYVCPIESDAYRAAEKAYEKVFGIRPLPV
                     RRGGSIGVVPVFEQILEVKPVLMGFGLESDAIHSPNENFPLDLFEKGIETIVEFYAQY
                     SKIR"
     gene            22224..23015
                     /locus_tag="PKH58_02440"
     CDS             22224..23015
                     /locus_tag="PKH58_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX87917.1"
                     /translation="MKRSYILSIASILFFLTGCTTQKELAYFNTITSGSTESLNAQIK
                     NARPEPKILVDDRLTITVSAVDPNAVAVYNLPFVSYASPGSDQIYSSPVLQSYLVNPD
                     GKINFPVLGEIKLEGLTLPEASYLIKMKLSEHVENPIVNIQFVNFRITVLGEVLRPGQ
                     FTIPNERVTLLDALGLAGDMTVYGRRDNVLITRNNNGKLEFARINLNSDEIFKSPYYY
                     LQQNDVIYVEPNTVKSISSQNIPLYLSSLSTLATLVALIYSISKK"
     gene            23060..25447
                     /locus_tag="PKH58_02445"
     CDS             23060..25447
                     /locus_tag="PKH58_02445"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNX87918.1"
                     /translation="MEKNNNINNTEIINLRELVSKYVKKWYWFVLAVIFAFIIATIYL
                     KKTDNKFQVQTVILLRNEDKTSEISQLAMMESLGLMGTSRVVDDEIQVINSNKILNQV
                     IDTLGIQAEYFEKNGLKYVEKYKSLPFELNIPVHFSDSLKAPVRIEVKTGTGSCKIKV
                     KYGEEFSKNYTLDNIQQPFSTPAGTLSFRINSTVKPGMKYKIKLYPKKELLAQFRSKL
                     NVSVVNKQSNAIRISIVESNIKKAEDILNKIVELYNLDAIIDKNIVATNTGNFIEDRL
                     VLITKELFDVESEVENYKKANALTDIASEAGIYLESASEYEKKIAELETQLNLIQYIE
                     SYIRENKNQYNLIPANIGIEDKSLMELILNYNSVLLERMKLSRTANEKNPVLMQLEQQ
                     IKVLKGNVLESISSVKSGLKIARNDVSRKDAQFNSRIKAVPTQERQFLEIKRQQEIKQ
                     NLYLFLAQKREENALSLASTAPAARTVDRAYASLTPVSPKRMIVYLVALILGLLIPFL
                     YIYLRDLINNKIEDVKEFQKTVKPPFLGSICISRESDRVAVQAGKTSTIVEMFRLIRT
                     NLQFVIGSKKNPAILVTSSISGEGKSFISINIAMSLALLNKKVVLIGMDIRNPVLKDY
                     MHLSNTNGQGVTLYLSDSSVQLRDVIIPSGYNSNLDVIPAGPVPPNPSELLLSSRLDE
                     LIMELKKVYDYIILDSAPIGIVSDTYLLNRVIDNCIYVARQDHTPREASNLINEIYAE
                     KRLNGMALVLNGTPATSAYGYGYGYGRSKNKFKNMPKLTFGDKLNESIMRLFHKD"
     gene            25667..26281
                     /locus_tag="PKH58_02450"
     CDS             25667..26281
                     /locus_tag="PKH58_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX87919.1"
                     /translation="MEKLAENIVYTAHQKFINFGIKSISVDDICNELRISKKTFYIHF
                     GQKESLIEAVLEYEMRKNTEVFEKMYKNKNAIDALIIIIKELKNRIDKEPDTFLHDLE
                     KYYPVTYAKFKDKQTVRIRSCFENNLKKGIEEGFYREDIDIELVSLFHSIQIRHTFDE
                     MQSFTPKIPKKRLVDFFIDLIVKLITNENGYRYMVENYYKIADN"
     gene            26298..27632
                     /locus_tag="PKH58_02455"
     CDS             26298..27632
                     /locus_tag="PKH58_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX87920.1"
                     /translation="MKKTVLFIFGLIFAGSVAAQDTISLSLPQAITIALSENPTIKIA
                     DKEIKRVEYNKKEKYGALLPNVSLGLSYARSLKKQKMFFSIPGMPSNPDGIEVGQDNT
                     FNGSTNGLMATLPLFAPALWQSINMSELDMELALESARSSKIALVNQVSKAYYAILMA
                     QDSYNVLKRTYENSTENNRIVQNKFKQGVVSEFESIRAEVQLRNVGANLSAAENGVEL
                     TRLQLKMLMGVGMDQEIKIEGSLADYENKMYSDVLKIDTTTLAENSDLKQFDIKSRQL
                     NQSVKLQRASWMPTLSASFNYNYMSYANDDILFTGDQRWFPTSNVGIMLSIPLFQGGQ
                     RYFKDKQLQIQVDELKDQKLNLKRSLELQAMSYINNIEKAMKLIESNKVALSQAEKAM
                     TISQKRYEVGAGTYLDVANAELAYQQSGLAYNQAIYDYLSAKTDLEKLLGNK"
     gene            27662..28681
                     /locus_tag="PKH58_02460"
     CDS             27662..28681
                     /locus_tag="PKH58_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX87921.1"
                     /translation="MKNYKLLTISLIAVFFGFISCTKTSKDKTQQKDAVLTVKLAQVF
                     ERDVPQNQEYTATVQPEIKNNIAPASPGRIRRILVNVGSRVAAGQRIVQMDAANLANY
                     QTQIDNLRRNYNRISELFAVGGSSQQEVDNVKAQLEQAETTLRNLNENTYLTSPISGI
                     VTAKNYDSGDMYSGQMPVLTVMKINPVKILINVSESHYSKIKAGMNVDIHFDVFPNQS
                     FSGNVSLIYPTIDERTRTFPVEIKLANNDSKVRPGMFGRVTINYGTEKRVVVPDQAVI
                     KQAGSGARYVYTYNDGKVSYLQVELGQRLENEYELISGVPSGAQVVIAGQAKLTDGAT
                     VKVVK"
     gene            28733..31858
                     /locus_tag="PKH58_02465"
     CDS             28733..31858
                     /locus_tag="PKH58_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX87922.1"
                     /translation="MKIYESAVKKPIMTILIFIGVMILGLFSLNKLSIDLLPDIETNT
                     IMVITSYQGASAADIETNVTRPLENTLNTVSNLKNITSSSRENRSIITLEFDYGLDID
                     VLTNDVRDKLDMVKTFLPDAAGDPIIFKFSTDMIPVMMIAATADKSLPALYKILDDNV
                     SNPLARINGVGSVSISGAPEREVQVFVDPAKMEAYNLTIEGIGQNIRLENVNTPVGNM
                     DIGSETYSIRVEGEFSDVSQLKSIVVGTYNGKTVYLSDIANVVDDKEERLQESYTNGV
                     QGAMIVVQKQAGANTVQIANEINKVLPDIKKNLPPDIKLSLINDTSQSIKNTIKGLAE
                     TVLFAFLFVILVVLFFLGRWRATIIIILTIPVSLLASFIYLYATGGSLNVISLSSLSI
                     AIGMVVDDAIVVLENITTHIERGSSPKSAAIHGTNEVSLAVVASTLTLIAVFFPLTLI
                     SGMAGVMFAQLGWIVTIIMTMSLICAMTLTPMLSAQMLQYNKNHTPFFKKVFTPIERF
                     LDGLDDGYARMVNWAVRHRWTVMALATIFFLLSVIPMAFVGSDFFPPQDNGRIAVNIE
                     MPVGTRMEISREVASKLEAEWRKKYPSLEIVNYTVGQASSSNIWGSMQSNGSNIVNMN
                     LGMIPLKDRSLSMFSLCDSLRRDLEVIPEIQKSKVIAGGGMNMMGGQSTLDVEIYGFD
                     FSKTDKIAANLKTELGKIDGLANINISRQDYVPEYQVDFDREKLALNGLNISTASNYL
                     RNRINGLTASLYREDGEEYNIRIAYDPKYRQSIEAIENILIYNAQGQAVRLKELGKVV
                     ERFTPPTIERKNRQRVITVASTLSGTTMDKAIKSINDAIAKMDIPGDVYLEVGGSYQD
                     QQESFADMGTLLLIIVLLVYIVMASQFESLVYPFITMFSIPFGISGVSLALFMSGQTL
                     NMMSVIGVIMLVGIVVKNGIVLVDYINLNRERGMGIVQAVVKGGRSRLRPVVMTTLTT
                     ILGMLPMALSNSQGSEMWKPMAVSVIGGLTVSTLLTLVIVPSLYAITAGMGMKRKMHK
                     LAKVNKQSARY"
     gene            31892..32185
                     /locus_tag="PKH58_02470"
     CDS             31892..32185
                     /locus_tag="PKH58_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87923.1"
                     /translation="MKTIFIPFDQAFKDQVIALLNENHVRGFTFWDNVQGRGSKTGEP
                     HYGNHAWPSLNNALLTVVDDEKVKPILEALKNLDKSSEMLGLRAFVWNIEEMY"
BASE COUNT        10202 a         6294 c         6818 g         9624 t
ORIGIN      
        1 tgtaatcagt ttggtaatca ggatcccggc accgatgaag aaattcagtc gttttgccag
       61 ttgaattacg gtgtgacttt ccccatgttc tccaaaatag atgtaaacgg cgataatgca
      121 catccgatat tttcttacct gaaaaactca ttatcaggta ctttaaccga cgatataaag
      181 tggaatttca ccaaatttct gattgatcca acaggtaaac cactaaaaag atatggttcg
      241 aatgtaaaac cggaatctat tgaggcagat ataattaaac tgttgaatcc ttctaaataa
      301 tttttacctc aaagctgtat atatttttct gtacaaaaaa aaatatttat ctttgcaccg
      361 taaataattg tggaaagatt tggactgctt gcaaccacga aaaaaaatgc taaattgtaa
      421 tttactttcc atgttttttt tcagtccttt taaatcaatc ctctgaattt taaattaatc
      481 tggtttgagc ctgttatttg catgctcatc aattaaattt ttattaaaaa atgggatatt
      541 tattttcttc tgaatcagtg tctgaaggac accccgacaa agtagccgat caaatttcgg
      601 atgctattgt cgataatttt ctggcacagg atgccaactc aaaagtagct tgcgaaacac
      661 ttgttacaac aggtcaggta gtattggcag gtgaagtgca tacacaggca tatgtggatg
      721 ttcagtcagt agctcgtggc gtgataaacc gtattggtta caccaaaagc gaatatatgt
      781 ttgatggaaa ctcatgtgga gttttatcgt cattacacga acaatctccc gatatcaacc
      841 gaggtgtgga acgtgaagac ccgatgaatc agggtgccgg cgaccagggt atgatgttcg
      901 ggtatgcatg taacgaaaca gttaactaca tacctgtggc tttagatttg tcgcacgcac
      961 tggtgaaaga actggcttac attcgtcgcg aaggaaaggt gatgacctat cttcgtcccg
     1021 atgcaaagtc gcaggtgact attgaatata acgatgataa ccaaccggaa agagttcaca
     1081 caatcgttgt ttctactcag catgacgaat ttgtaaaacc ttcagaaggt aaaacacagc
     1141 aacaagctga tgaagaaatg cttgccgtca tctataacga tgtgaaaaac atattgatac
     1201 cacgggttaa agccaaagta atacagtttg accagctgat aaaagatgat tatatcctgt
     1261 acgtaaatcc gacaggtaaa tttgtgatcg gaggcccgca cggagatacc ggacttaccg
     1321 gccgtaaaat cattgttgac acttacggtg gtaagggtgc tcacggtggt ggtgcatttt
     1381 caggtaaaga tccctcgaaa gttgaccgtt cggcagcata tgcagcccgt catatagcca
     1441 aaaatcttgt cgccgcgggc gtttcggatg aagtgctggt acaggtcgct tacgctatcg
     1501 gggttgccaa accgatgaat atatatatta atacctatgg caaatcgaag gtaaatctga
     1561 ccgatggaca gatagctcaa aaagtggaga aactctttga tatgcgcccc aaagccattg
     1621 aacagcgatt gaagctgcgt accccaatgt acgaagaaac ggcttcgtac ggtcatgtag
     1681 gacgtacacc gatcattaaa gaaaaaacct tcggatataa tggtaatatg aaaacagtga
     1741 aagtggaatt atttacctgg gaaaaactgg acatggtaga tgaaatcaaa aaggaatttg
     1801 gtttgtaatt tcaacaataa attaattttg agagcttcaa tttatttgag gctctttttt
     1861 ttggaaaaat atatgatttt tataaacatt tttttaattc cattccatgt ttttttgatt
     1921 acaatatttg atatggaact taatatttaa aacatttaga ttacttttgc gtaaaaaata
     1981 taaggagatg aacagggacg aaatttatag tcaaatcaac cggatttacg gatttctgaa
     2041 agtaaaactg tttcagttta taaactggcg cgaaaagcgg atcattcgtt tttacggact
     2101 tccgaaatgg agaaaagtat ccaatacaat ccttacgttt ttcggtttgt tcttgtttta
     2161 cttaattctt gttgatatca attttttatg gctctttggt aagtcgcctt cgctcagagc
     2221 tataagcaat ccaccacagt ccttatcttc cgagattatc agcgccgatg gtaaacttat
     2281 cggtaagtat ttcaatgaaa accggaaact cgtaaaattt gaggatattt caccaagtct
     2341 gatcaataca cttatcagta ccgaagacca ccgtttttac aaacatttcg gagtggatat
     2401 ccgcgggctt tttggtgcca tgaaagatat gattcagggt aatccgcgcg gggcaagtac
     2461 catcacacaa caactggtga aaaacatgta caaaacccgc agtcaatact ccagaggatt
     2521 acttggttca ttgccgggtc tcagacttcc cctcgaaaaa actaaggaat ggactgcagc
     2581 tcttaaactt gagatttttt attccaaaaa tgatattctg gagatgtatc tgaatacagt
     2641 tagtttcggt agtaatgctt atggaatata tactgcttcg aaaacctatt tcaataccac
     2701 tcccgataag cttaattatg agcaatcagc ggtactggtc gggttactta aagccatcac
     2761 caagtataac ccttacatta atcctaaaaa ctctcttaaa agaagaaatg ttgtattgaa
     2821 tctgttggtt cacaataaat atattactca gcaggctgcc gattcgttaa aagaaattcc
     2881 aatccagctg aattataata ttgaaaaacc taatcaggga attgcaccgt atttcagggc
     2941 gaatttaacc cgttttctgg aaaaatgggg agaaacaaac ggttatgaca tatatgccga
     3001 tggattaaag atatatgtaa caattgattc acggatgcaa caatacgccg aggatgctgt
     3061 cagaaaacag atgaaacaat tacagaggaa cttttttgcg cattggtccg gacagaatcc
     3121 atggcgcgat gaaaatggaa atgaactgaa aaatttcatt tctgacattg ccaagcatac
     3181 aagagtatat cgtaatctgg cttcgaaata caataacaat caggattcga tagataagta
     3241 catgaacctg ccccgcaaaa tgaaggtatt tgattatgat aaaggtgtaa aaacttttac
     3301 catgagcccg atggattcta ttcgttatgt gaatcacttc ctgcattgtg gatttgtggc
     3361 tatggaacct gataccaaat atgtaaaagc ctgggtaggt gatgtggatt atgataactg
     3421 gcaatacgat aaagtggcgc aaagtaaacg tcagcccggt tccaccttta aacttttcgt
     3481 atatactgcc gcgatgatag caggaaaatc accatgcgac aaaataactg acaagccaat
     3541 aacctggggt acaggcgaag atgcctggag cccgaaaaac tctaccggtg gtttttctta
     3601 cgaagatatt accttaaaaa gcgcctttgc gaaatcggtg aataccgttg ctgctcaact
     3661 ggctaaagaa gtaggacttc ataatgtcgc aaagtgtgct catttattgg gcattaaatc
     3721 caaacttgat gaagttccca cgctgagtct cggtgcttct gacgtatcac tgcttgaaat
     3781 ggtcaacgcc tacagtactg tcacaggtga aggtatgtat cattcaccaa tcattgtcac
     3841 aaaaattgtc gataaggacg gctatacaat ttacgaagaa aaaataaaac ccaaaagagt
     3901 cctatcctat gaaaatgctt tcttgatgac agaactgctc aaaggaggta ttactgaacc
     3961 gggtggaact tcgcgggcac tttggaattt tgacctcatg agatgggata ccgaattcgg
     4021 tggaaaaaca ggcacttctt ccaactattc cgatgcctgg tatattggtg ttaccccaaa
     4081 actggttggt ggcgcatggg ttggagctga acatcgaagt gttcacttcc ggaatggaag
     4141 catggggcag ggaagtcgta cagcattgcc tgtttttgct ttatttatgg aaaaagtgct
     4201 atcagataaa cagctgtcgc attaccgggc taagtttccg gcggaaccaa aacagcctat
     4261 atcgcgtgat tataaatgtc agagttatgt taaacctgat acagcgcttg ttgacagtac
     4321 catagtcgaa ccgattgaaa cattgcctgt tcctgacctc gatttgagta aaccggatga
     4381 aacgaccaac ccggtaactc cacaataatt tataaatcag agtaatacca taaaaaataa
     4441 tttaacaaat ttcttttttt aaatagatga ataaaattat aagtaaagaa tatttttcag
     4501 caaatgttgt gaaatttgtg gtcgaagcgc cactcatagc aaaagcgcga cgagctggac
     4561 actttgtaat agtaagagta ggagagaagg gcgagagaat tcctttaacc atcgccgatg
     4621 ccgatatcag taagggaact attacccttg tgattcagcg aatgggtgtt tcatcgacaa
     4681 aattatgtgc tttagaagcc ggtgattata taactgacct tgtcggacca ttgggaaaag
     4741 cgactcatat cgaaaacttt ggaactgtgg tttgtgcttg cggtggggtg ggaacagctc
     4801 cgatgatgcc aattgtagct gcattgaaaa aagctggaaa taaagtgatt acagtgcttg
     4861 ccgcccgtac aaaagaactg ataattcttg aagagcagat gcgcgaatat tcggatgaag
     4921 tgatcattat gaccgatgat ggttcgtatg gcaacaaagg attggtgacg aatggcattg
     4981 aagaagttat ccaacgcgag aaagtggata tgtgtgtaac tataggacct gccataatga
     5041 tgaagtttgt aagtatcctg acaaaaaaat atgaaattcc tacagtggca tccctgaaca
     5101 cgattatggt ggatggtaca ggaatgtgtg gagcctgccg tgtcacaata ggtggtaaaa
     5161 ccaaatttgt ttgtgtggat gggccggaat ttgatgctca tcaggtagat ttcgatgaaa
     5221 tgctgatgcg gttgggaggc tacaaagatc aggaaaaaga agaaatggaa cataaaattt
     5281 tgcatacgaa ataataccta tgaataccaa aagagatgaa caatggcgcg tagatttgcg
     5341 ggcaaagatt aaagctaaag acagaaccaa ccttgagcgt gttcatatgc ctgagctcga
     5401 tccggagtat cgtagtcaca tcagaaatga agaagtaaat ctgggtctga cacctgaaca
     5461 ggcaatggct gaagcccagc gttgtctgga ttgtgctaat cctacctgta tggaaggttg
     5521 tccggtagga attcaaattc ccgattttat taaacatatt gaaacaggca attttttgga
     5581 agccgctaaa gttctgaaac ttacttcggc acttccggct gtatgcggac gtgtttgtcc
     5641 tcaggaacga caatgtgagg aaaaatgtat tcatttgaaa atgaagaaag acgctgttgc
     5701 aatcggctat ctggaacgtt ttgcggcaga ttacgagcgc gaaagcggtc agatttctgt
     5761 tcctgaaaca gcgcctgcaa atggaataaa aatcgcagtt gtagggtccg gacctgccgg
     5821 tctggctttt gcaggcgata tggccaaata cggttatgaa gtccatgtgt ttgaagcttt
     5881 acacgaaatt ggtggtgtat taaagtatgg tattcctgaa tttcgtttgc ccaataaaat
     5941 tgtagatgtt gagatcgaaa atctgaaagc gatgggggtg atttttacca ataactgcat
     6001 aattggtaaa acaattgcca tccaggaact ggaagaaaaa gaatatcagg gcatttttgt
     6061 ggcaagtggt gccggactgc cccggtttat gaacattaag ggagaaaacc tggtaggcat
     6121 tatgtcgtcc aacgaatacc tgacccgtgt taatctgatg gatgctgcca atcctgattc
     6181 cgatactcct gtttacaaag gaaagaaagt cgctgtaatt ggtggtggca ataccgccat
     6241 ggattcggtt cgtactgcac ggagattagg tgccgaaaaa gcgatgattg tgtaccgtcg
     6301 gtctgaagaa gaaatgccag cccgtgtgga agaagtaaaa catgccaagg aagaaggaat
     6361 cgaatttctg actctccata atccgctgga atatatagga gatgccaacg gacgggttac
     6421 gcagatgata ttgcaggaaa tggaactagg tgaaccggat gaatccggac gccgttcacc
     6481 ggtagaaata cccggaaaaa cgaagacgat tgaggttgat gaggtgattg tcagtgtggg
     6541 tgtatcacct aatccgttaa tcaaacagtc ggttccggaa ctcgaaacta ctaaatgggg
     6601 aactttggtt gttaatcagg atactatgca gtcagtttca atacctataa tttttgccgg
     6661 aggcgatatc gttcgtggag gtgctaccgt tattctggcc atgggtgatg gacgacgggc
     6721 agcaaaagca atggacgaat ggattaaaag taaaggataa tttgttaaaa atgaaatatt
     6781 aacgccgtaa ttcgttatta aacggattac ggcgtttttt tactttgttt aaagcttaaa
     6841 aattatacac agctactcca accactctgt gattggtcac agaataagga tcggtaactt
     6901 ttccctgagt gttaagtttc ccgtatccgg cattagttaa atcatactcc agacctaacc
     6961 gaatgttttt catattgtag gagaggtgcg gcgcaatcct gattaattga tccagatgct
     7021 gattgcccga aaaataaccg gaactataga cagtataatc attattagca gtcggaagta
     7081 gatttttatc tgttccaaga tttttaagat aacctcccaa aatacctact tgccaggttt
     7141 ttccataaac cagattaatc cagctggctg actggttgaa gttggtgtat gtatgtaatt
     7201 tattttcaac attatagcta cttaagccat agcccatagg catttgcaaa tcgctcagat
     7261 tctgaccaat caatgatttc gctttgattt gtaacttctt actggcgtat tgtccgtaaa
     7321 tcatggcgct ttttgaacca agcctgcttt tgtttatttt taaagtttta taatccagtc
     7381 caatacctcc ggtaaagtgt tttccggtgc tttccatgcc cagatacata tcggggagca
     7441 aggcttgttt catgtaattg ttcgatttgc cttcaggtcc gtaggatgta tattgcatct
     7501 gataaatacc ggcaagacta acagacaggc ttgatgtcag gttttgtttc actctcagtt
     7561 gaggacttcg attgaagggt tgaaaaggtg atccggagtt taacgataca attgtaggag
     7621 ctacatttcc gtagagcgga tgccacgtct gaccagcaag cagttctgta tttttccagt
     7681 tcagcttcat atacgcctgc cgaagacgaa ccaaaaaata agtggtgcct gtaccgcaaa
     7741 aatccagttc aattttagcc gtagaacggg ctttaaatag ttcaggacca ttgatgttca
     7801 gacctaaacg gcttgctaca ctgatcattt cagcctgagc tgtgttgttg atatcatttc
     7861 cgtcagcatc gtgtactaac ggttttggaa atatgttaaa tattccatcg ataacctcca
     7921 cattttgtct ggagttataa taaaagtcat ttctcacaaa gccgtaaagc tcatattttg
     7981 gtgctgtctg ggctttgccg gtgaaaagga aagaagagat aatcagaaaa attatcaacg
     8041 cgaaagtata gttattgtac ttcatagttg gttatgttta acatccgtca cagatgagta
     8101 attttataaa aagagatgtt caagcagtat ttttgtgcca attccaatca gaatgatgcc
     8161 accgagtatt tccacattga atttaaacct tttaccgaac cggatgccaa ttcccatccc
     8221 caaaaaggtg aagagaaaac tgacaattcc gataatcaaa acggcagata agatgatatc
     8281 agggaatgga ataaaaataa tacccgtggc aagtgcatcg atacttgtgg caaaagccag
     8341 tgaaatcaat aacctgagtt tgaaaggatc gggagtgatt tcgcagtccg gatctatggg
     8401 tttcagacct tctatcagca tttttccccc tattatgcct aataatccaa aagccaccca
     8461 gtggtcgaaa ctgctcaact catgaataaa cgattttccg gcataaaatc cgattaatgg
     8521 cattattccc tgaaagaaac cgaagagaaa tgccatcttc agggtgtttt taaatgaaaa
     8581 tttaccgaca cagattcctt tgctgatgga tacagcaaag caatccatgg caagtcctat
     8641 gctaatgagt aatattgaaa gaaaatccat ctgatactta gtaaaaaata tgctaatgtg
     8701 tcaatcagtg attggcaaca tcagcatatt agttatttga ttaatttatt ataataatga
     8761 ttttacctgc ttgtaaacat tttcagcggt aaatcctaac ttttcgtcga gaattttgta
     8821 aggtgcggag tacccaaatg attccaatcc ccagattttt ccattatcac ccaccagacc
     8881 aagcagatta acgggaagtc ctgctgtcaa accgaatttc tttacgtttt tcggcagcac
     8941 ggtttcctga tattcttcgg attgtgaacg gaaaagtcct tctgaaggta cagaaacgag
     9001 acttaattta ataccatccg cacgaagcaa cgcagcacct tcaaccagtg tggcaacttc
     9061 ggaaccggat gcaagtagta ttacatcggg attttcgtca ctttcaacga tgtaggcacc
     9121 tttttctgct tttttagctt cttctttgcg gttagctgca ggcagatctg tgatgttctg
     9181 acgtgaaagg ataagtgcgg aaggggtttt ggtgttatca agcgccattt tccatgcaac
     9241 agtagtttct tccacatcag ccggacgaag taccagcatg gagttttgtc cgtgatggtt
     9301 tttcagtttt tccatcaggc gaagctgagc ttcctgttct accggttcgt gtgtcggacc
     9361 atcttcacca acacggaaag catcgtgcga ccagatatat ttcaccggca gctgcatcag
     9421 tgcagccata cgtacggcag gtttcatata atcggagaaa acaaagaaag ttgcacatgc
     9481 cgcgataacg cctccgtgca atgccattcc gttgcacaga caggccatgg taagttctga
     9541 aaccccagcc tgcaggaatt tacctgtaaa gtcaccttta gcaaatgctt ttgttttttt
     9601 caggaaacca tcggttttgt ccgaattact caggtcggca cttgacacga tcatgttttc
     9661 cacatgttgt gcaagatagg agagtacggt ggcagaagca gcgcgggtag cctgatttgg
     9721 cttttgttcg atctcatccc agtttatttc cggagcattg cctgagaaga actgtgctaa
     9781 ttttgctgcc agttcggggt ttttggcttc ccatgctttg gctgcttgtt ttttttcagc
     9841 cattatttct ttcagctgaa cttcacgttt ggcataaata gcttttgttt cctcaaaaat
     9901 tacgaaagga ttttcaggat caccgcccaa atttttcact gaatcgacaa acgatgctcc
     9961 gcttgctccg agtggctgac cgtgagtttc tatttcattt tcaaatgatc tgccgtccga
    10021 tgctacagca cctttagcca tgatagtttt gcctataata attgttggct tttctttttc
    10081 agctttagct tcacgcaagg ctactcttac ttcagttaca tcatttccgt taatttcaat
    10141 cacattccag ccccaggcgc ggtatttcat tgctacatct tcgcttgtaa cagagtttgt
    10201 gtaagttgat agctgaacat tgttggaatc gtaaaacatg atcagattgt ccagtccgag
    10261 gtgtcctgct atacgtcctg caccctgcga aatttcttcc tgtactgcac catccgagat
    10321 ataggcatag atagtctggt tcattacttc tccaaaacgt gctttgagaa attttgcggc
    10381 aattgcggca cctaccgcat aggtatgtcc ctgaccaagc ggtcccgatg tgttttctat
    10441 tccacgtgaa agatcatatt ccggatgtcc gggagtgatg cttccccatt gacgaaactg
    10501 ctgcaagtct tccatactga attttccggt caaagccaga actgaatata gcatggcgga
    10561 catatgtccc ggatccaaaa agaaacggtc acggccttcc cagcagggat tttcgggatc
    10621 atattccaaa aattcggaat aaagtacatt gataaagtca gcgccaccca ttgctccgcc
    10681 gggatgtcct gatttagctt tctcaacagt ggcagcggat aaaatccgga tgttatccgc
    10741 tgcgcgattc aaatcttgtg tagtgtgcat aaaaaatatg aatttaagtt atatcctgat
    10801 taaaaatgat atccaacagt atagctgaag ccccaggctg atgtttctcc tctaccgtat
    10861 ccgggaatat accagggtgt aacagcaccg tattttccgt tgttaagcag atgtttattt
    10921 cgaacattcc agcccagata cagatttttg aagaattcaa cccgaactcc ggctacaatc
    10981 tcaaaccaga ttttggtagc tggcatggag taattgatag tttcagtccc tccccaataa
    11041 ttgtcttcaa ttgtcagatt atcagttgta tagttgaatt gtgacatgcc tattcgggca
    11101 ccggcaagga aatagttgtt attgatttta gtgcctggtt tcggttttag taaattgaaa
    11161 tcaactccta gtttaccaaa catcccttca cctctgaaag ttgtgttgtt ggtcagattt
    11221 ctggttgcgc ctcccatccc aatttctacc accggtaagt acgaattctt tagattagcc
    11281 tgtacattgc cctgatacga atagacgttt ctggttgaat tattcgtaaa ggcattctca
    11341 atgaatggtt caatgtcaat ttcgatatta ataccctgcc agacgggtat ggtggtagtg
    11401 tctttttttg attgaatagt tgtttttgca ttcgcaagcg aattattttg aggctgttgc
    11461 gcctgcgcta atccggcgaa gagaataaca ctactcagca agatatattt taatgttttc
    11521 gacataagta gtgttaatga ttggttgaat aattttgatt ttttcgatga tgtgattggt
    11581 tgtcagtact gtatcaatgg tatgtgtacg aacacaaccg cattcgagcg aaaggtattc
    11641 gtctgtatta tgataaagaa cggttattgt atcatatact tcattgaatt ttatcgagaa
    11701 acgactctga gttttcagtt tatcaacagg tagctttatt tcactgagat tcgtaacttt
    11761 tttataaatt atagagtcat tattaacccc tgaagcagtc agactgtcag ctttaaagtt
    11821 aattgtaacg tattgattgg ttttcaggtt gagggtgtcc tgatagaatt tagcgcccat
    11881 aagcacggtt ttgcttttgc ggcaggtttc atcattattg cacgatatca atcccgaagt
    11941 gagaagtaat accggtaata gccaaaaata tagtttcttc atgctgaatt ttaaataatt
    12001 tctttcactt catctttcaa tacctgcata gcaacatcca gggctgtttc atctaccgaa
    12061 tcgtaaagtt tgataatcat agtcgccagt tttgcagcag attcttcggc ctgacattgc
    12121 gatgcacagg tattaatcag ttgaataaca ttctcatacg cgtctttaat tgattcccaa
    12181 agctcactgc taacataaat ctgctgcgag aggttgtgct caaattcacg ccggagatcg
    12241 ttaagaagca cctgctgaag ttcaaaacaa ttcatgccgg cttctacttt gttgactatc
    12301 actctgttgg gatgaatccg ttccatcaac agcattaatc tttcgtacgc cttcaaccgg
    12361 atcggggtaa cagtagtatt gtttgctttt ttcaattcaa aattccggcg ttctgcttca
    12421 tttttgaaaa gcagatttag taacaaatag gttaccagta ctaccagcac cgcaggaatt
    12481 gtatatttta atatttcaat ccacataata tctatttctt tttacccgat tgatgaatta
    12541 ttatatcgat tggctgcata taaaccaggc aaccctttcc gaacgcctca atatcgcttt
    12601 ttatatcctc aataaagttt tcaagcactt cagtgcggtc aatcatttct ataattacgg
    12661 gaagtttttc attgatttcc caaaatttgc cggatacaat cttattgccg ctcaatccgt
    12721 aacctgttat gcctctgtaa gctgtagcac ccgaaattcc ttttttataa gctttcatta
    12781 ccagatgttc gtagaataaa ctcatcccga atttgtcggt attgcttaca tatatcttca
    12841 aaatgctgtt ttcgcttttc atttttacag aagattagag attaaatatc cgagataaac
    12901 cgctataact ccaaaaagca aacttccgag ggcataaaca gcggctgtca gaaactgtcc
    12961 attctggagc aactgaaaat tttcattagt aaaagaagaa aaggttgtaa atccgccaca
    13021 gatacccact acaagaaata gcttaaggtt ggtgttcaac agatcgctgt ttacaaaaat
    13081 tcccagaaaa aatccaatca gaatacttcc aagaatattt actgtcagcg ttcccattgg
    13141 tatgtcgtga aaataccagg tcagattaag ttttgagacc agataacgtg caatgctccc
    13201 gataaatcct ccaagcccaa ctaataataa ttgtttcata attaattagt gatcaattga
    13261 ttaaattaac cggatgttca atgcaaattt gaaagtttaa acttcttttt ctattttata
    13321 attgtttctt tcgggtgcat ttcgggtaat aagttctccc agaaaacctg tcaggaagag
    13381 caaagtaccc aaaatcattg ctgtaagtgc aatataaaaa tacggactgt ttgtaaccag
    13441 cggcgaagga atgtgtgcgc tgagtgcaat tagttttttt actccgacaa caattacagc
    13501 aaaaaagcca agtataaaca tcaggctgcc tattaaacca aaaaagtgca tgggttgctt
    13561 cccgaatttg gaaagaaacc agagggtaat caaatccaga tacccgttga caaaacgatt
    13621 catcccaaat tttgaactgc caaactccct tttacggtgt tcaaccactt tttccccgat
    13681 tttagtgaaa ccggcatttt tagccagata gggaatgtag cgatgcattt cgccgtaaac
    13741 ttcaatattt tttacgacat cccggcggta agccttgaga ccgcagttca tatcgtgcag
    13801 tttcagtccg gtaaccctgc gggctgtggc attatacaat tttgaaggaa gatttttggt
    13861 cagtttggca tcataacgtt tctttttcca acccgaaacc aaatcataat catcttttgt
    13921 gatcatcgaa tacaattcgg gaatttcgtc gggactgtcc tgcaggtcag catccattgt
    13981 gattaccacg tcaccttcgg cagccctgaa tccacagtat aacgcgggcg atttaccata
    14041 attatgcctg aatttaatcc ccttaacgtg tggagactga gatttcaact gctcaataat
    14101 gcgccacgaa ccgtcggtac tgccatcgtt tatgaaaata atttcataac tgaatttatt
    14161 atcagtcatg actttgtcca cccactcgta gagttttgga agagaatctt cttcgttata
    14221 caatggcaca ataacagaaa tatccatatg gtgtaaagtg ttaattgact gtgaaatatg
    14281 atttattaat tttccggatt gttaaaaata tctttgtctt tttttacaaa tggcgcaatg
    14341 atcagcccca ttattacacc cagaaacgta ttaacccaaa tatactgaag tgtaaatgct
    14401 gtaggactca tcattttatc catcgtttca taataatcat caggtacgct caggtgaaac
    14461 tgttcctgta actgttgcat catactgaca ctttgttcaa gaagcatagg caagtaattt
    14521 acattcagat acttgaaata gatgaatttg aaaagagagg atatgatggc tgcaaaaaga
    14581 aaaagcagga tgataaagaa aaacgcatgg ccaaaactaa tggagccacc gttttcctta
    14641 tcgcggtaag ctacagcaaa ccgataggta agaacgaaga taaacgctac caccaaatat
    14701 ccaagaagag taattatagc attgccagac accgaaagca aaaagttaac agaaaaaaca
    14761 atacccataa tcaacccgta tttcatggct gcatttaata catttgtttg cataaattat
    14821 ccgctttgat tttgctacaa aaatacaaaa aaataaggaa agatataatg tttttataac
    14881 attgacaggt gcacaggcaa taattaatca ttagatgtgt aaaactatta ctattttcaa
    14941 tcagaatatt taattttcaa ccgatttgcc aaatatcgat ggcattctcg tgttttagaa
    15001 taataaaaca gaatcgtata aagtaaaatg acaaaagtct attatagttt attgtttttc
    15061 tcaaagtgtc gacaccagga ttttattccg cattcttcgc attttggttt ccgtgctatg
    15121 catacatatc gcccgtgaag aattagccag tgatgtgctt tcggaattaa atgatccgga
    15181 atatatttta caagctcacg ttcggtttcg agaggtgatt tagagttcgt tgtaagcccg
    15241 attcgttccg aaatccggaa aacatgtgta tcaacagcca ttgcaggttt attgaatacg
    15301 acagaggcaa taacattagc tgttttccgg ccaactcccg gtaatgtctg caatttttca
    15361 acatcatcgg gaacaatacc attaaagttg ctcacgagtt tttttgacat acctatcagg
    15421 tgtttcgctt tgttattggg gtaggataca gacctgatgt attcaaatat aacttcggga
    15481 gttgatgctg ccattgcttc cgcggtgggg tagtcgtaca gaagcttcgg ggtgaccata
    15541 ttaacccgtt tgtcggtgca ctgagccgat agaattacag ctacaagcag accgaatgga
    15601 tctttataat ccagttcagt ctcagcaatg ggtcggtttt cttcaaacca ggcaataata
    15661 ttttgaaatc tttgttgttt cgtcatgtga ataattattt ttggcaaatt taacataaag
    15721 catttatatt gccattcatt tttcgtaatt ttgcaaatta gaagaatttt aaaagatata
    15781 aataagcata tatatcccga taaataataa gtatgcctcc aaaaaaacca gttaaaaaaa
    15841 atacagctga gaagccacag cttacgcgaa ctaagcagag aacttcagag ccgggtttgt
    15901 tttcaaaagc ccttcatttc ttacgggatg ataaattaca tttcatcttc ggattgctgt
    15961 tgtccattgc tgtcctctat gcattcctgt cttttgtttc ctattttttt acaggagcag
    16021 ctgataaaag tattttcgac aacattagtt tcagagaatc gttgaggata cgcggatctg
    16081 taactaactg gacatccgtt atgggtgcat atatttcaga aacactgatt gataactggt
    16141 ttggagtttc atcagccgcg atattaattt ttcttctggc tgtagcatta aggttgatga
    16201 acgtgaagat tatttccatt tggaaagctt tttttcactg ctttttctgg cttgtgtgga
    16261 tatcggtttc actgggattt attaccgatt ttattccttc cctgcaaccc tctttttttg
    16321 cacttggagg acaacatggc acttacacgg ctgttcagct taattcgttt gtggggcgtc
    16381 cgggcgcgtt gatgataatt gctggtgcac tgcttattta tctgattgta gtgtggagtg
    16441 ccacgatacc gtatatccaa aatttattca ataaaaacga taatatacaa tcagaaagga
    16501 aacatcagga accaattgtt gcagtaggta ctgaaattgt tcctgaagaa tggctggtat
    16561 cggaagtatc taattccaaa gaaacatata ccgaaaaaga gagtagcagt ctgattgaga
    16621 atgaaccgat agatttgaac gaagttgtgc agtttgaagt aaccaatccg gtgaaggatg
    16681 aaaccaatcc ggttgaagag tacgaagatg aagaaaacac tgatccgaat tataatgttt
    16741 caaaactggg aaaatatgac cctacacttg atttgtcgag ttacgtttct cctacattga
    16801 atttgttgaa agtctatggt tctgaaaacg acatgcagat tgacatgaaa gagcagactg
    16861 caaataaaaa taggattatc agcaccttgc gtaattttgg aattgaaatt gacagtataa
    16921 aagccaccgt aggtccaacc attacacttt atgaaatagt acccaaatcg ggtatccgca
    16981 tttctaaaat ccgaaatctg gaatcggaca tcatgctcag tctggctgct actggtattc
    17041 gtattattgc tcccataccg ggtaaaggga ctattggtat tgaggttcct aataacgagc
    17101 ctcagattgt atccatgaat tctgtcattg gttccaaacg atttactgaa gctaaattcg
    17161 agcttcccat tgcgtttggt aaaacgatta ctaacgaggt gttcatggtc gatctgacca
    17221 aaatgccaca cctgctggtt gccggtgcta ccggacaggg aaagtcagtc ggtttgaatg
    17281 cgatcataac atccttgctt tataaaaagc acccgtcgca gttgaaattc gttctcattg
    17341 accctaaaaa ggtggaattc aatatttatt cggctatcga aaaacatttt cttgcaaaac
    17401 tgcctgatgg tgaagatgcc atcattacag acaccagcaa agtggttcaa acattgaact
    17461 cactttgtgt ggaaatggac gaccgctatg atttgctgaa aaaggcacat gtgcggaata
    17521 taaaagaata caacgaaaaa tttgtcaaca gagaattaaa ccccgaaaag ggtcacaggt
    17581 ttctgcctta catcgtggtc atcgtcgatg agtttggtga cctgataatg accgcgggta
    17641 aagaagtgga aatgccgatt gcgcgtattg cccagttagc ccgtgcagtg ggaatccata
    17701 tgattattgc cactcaaagg ccatcagtaa atatcattac cggtgtaatt aaagccaact
    17761 tcccggctcg tgttgcattc agggtatctt ctatgattga ctcgcgcacc attcttgatg
    17821 cacctggtgc caatcagctt gtagggagag gggatatgct tttctcacag ggcagcgatc
    17881 ttatccgcgt acaatgtgca tttgtagata cgcctgaagt ggaacaaata gcgctctata
    17941 tcggaaatca gcgtggctac ccgacagctt tctatctgcc cgaatacgta ggtcccgaaa
    18001 gtgaaggtgt cgatacttca actgttgata tgagtaaacg cgaccctatg ttcgaagaag
    18061 ctgcccgtct gattgtggcc agtcagcagg gatcaacctc catgatacag cgtaaatttt
    18121 ctatcggata taaccgcgca ggccgcattg ccgatcagct tgaggttgcg ggtattattg
    18181 gcccgaatga aggtagcaag gcacgacagg tactggtgca gacagaatat gaactggaac
    18241 agatactgaa acatctgtaa cagagagatt gtaatggccg attcattatc aactgattac
    18301 aacaaattat tttttaccgg ttaataaaga atataaattg gcatacaagt tgctggttga
    18361 aggatataat cctataattg ataaaaatga atataaaaaa aatagttttt agtttgatga
    18421 ttgccattgt ggcatttacc tccattaaag ctcagagcaa ttccggtgcg gtaaaagtag
    18481 tcgataatat gcttcagttg cttaaaaaca atgcgataaa gacaaacttt gaaatggtgg
    18541 ttaaagaacc ttcctctcct cagtcacacc ggatgaaagg taactttacc atgaaggaaa
    18601 ataaattcat tctgaataca gccgaaatga atgtttattt cgacggcaaa acgcaatcgg
    18661 catatatgac atcggtcaat gaagtgtcca tcactaatcc gactgaaaag gagctggctg
    18721 aaacgaatcc gatggcaata atatcatctt ataaaagaaa atcaacaatt aagtttgtta
    18781 gaagcagcat ctcaaagtcg tttcataata ttgagttgat tcctaaggat aaaaattcgt
    18841 cattcaaaaa gatagtattg aatgtgaaca aaaccaataa ttatccgttg tttattcaac
    18901 tgattgataa gggcggtatg attaccgatt taacattaac gcagtttaaa agcggactaa
    18961 atatcagtga taacgctttc gtcttcaacc ccaaaaaata taaggatatt gagattaatg
    19021 atttgcgata acagatttga aaatttgtta ttggctcgtg ttgaatgcgt tagtttgata
    19081 gtcactttaa attaacgcat ttaaatttta aaaatagtaa aacaataaat aataaacaaa
    19141 aacaagaaaa cgaatgaacg aaaaagtaag atgtctgatc attggatcag ggcctgctgg
    19201 ttacacagcg gcaatttatg cttcaagggc taatctgtcg cccgttttgt atgaaggaat
    19261 gcaacctggc ggtcagttaa caaccaccac cgaagtagaa aattttcccg gatatcctga
    19321 aggtatcacc ggacccgaac taatggaaga tttgaaaaaa caggctactc gttttggggc
    19381 ggatatccgt tttgggatgg ttacagctac cgatttgtca tcggctcctt acaaaataac
    19441 catagatggt gaaaaagtaa ttgaagctca aacattaata atttcaaccg gagcaacagc
    19501 caaatacctg ggattgccgg atgaaacaaa atatgccggt tcgggtgtgt ctgcctgtgc
    19561 cacctgcgat ggatttttct atcgtaaacg caaggttgca gtagttggtg gtggcgatac
    19621 agcagccgaa gaagctgttt atctggcaaa tattgccgaa aaagtatatc tgattgttcg
    19681 taaggattat ctgcgggctt cgaaaataat gcagaaacgt gtcatggaaa atccaaaaat
    19741 tgaagttctg ttcgaacatg aaacactggg tctgacaggc gatatggtag tacagggtgc
    19801 tgatctgatt aaacgcagag gaaatccaga cgaagaaaaa gtccatattg aaatcgatgg
    19861 tttttttcta gcaatcgggc atactcctaa ttcgcagata tttaagccct gggtcgaaac
    19921 ggatgagaca ggctacatta aaacaatacc cgggacaccc aaaactaatg ttgaaggtgt
    19981 atttgcagcc ggtgatgttg ctgatcccca ttataaacaa gctattaccg cagccggaac
    20041 aggttgccag gcagctattg aagttgagag gtatctttct gaaaaaggtt tatagcgtga
    20101 cgacaatttt acataaaaaa agcagcgtta agctgctttt tttatgagta ataattctat
    20161 tttatttctt cgagcatgcg cagagcgact tccagtatgc ttgtatcgtc cagttgaaca
    20221 atacctccgt caggccccgg ttcgatatgc agtcctacac tctttccctc tttataatca
    20281 ccacttccga aataattctt tactgtttgt ttttccaggc caagttcttc ggcgatcttg
    20341 gccatacttc cgcttggcag agcatctttt attctgcgca attcgttaaa agttattgtc
    20401 ttagtcatag caaatggaat tttatagtta atacttttgt gtttatattt tgagcaataa
    20461 acatagtgtt ttaattttac ttttccaaac tttatggtga aaaaaaacct atttttttta
    20521 caaatgcatt aaagctcagt tacagttata tgatattcaa tcaattatct gattttggaa
    20581 tattgagcgt aaaattccac aattgtttca attccttttt caaataaatc cagcgggaaa
    20641 ttttcatttg gtgaatggat agcatccgat tccaaaccga atcccatcag aactggtttc
    20701 acttccagaa tttgttcaaa tacaggcacc acaccgatac tgccgccccg ccgtaccggt
    20761 aaaggtctga tgccaaatac tttttcgtaa gccttctcag cagcccggta ggcatcggat
    20821 tcgatggggc aaacgtaact ttcagcaccg tgaagctggc gaacttttac atcgatatat
    20881 tcgggagcaa tctgcagaat gtgtttctca acaagcttag caatcttttc gtatttctga
    20941 tggggtacaa gccgggccga aagtttagcg aaagcttttg acggaagtac tgttttactg
    21001 ccttcgccgg tatagccacc ccagataccg caaatatcga gagcgggacg aatgccggta
    21061 cgttcgatgg tgctgtaacc cacctcacca aatacctgtt ttattcccag attggcttta
    21121 tattcttctt cgctgaatgg tatcctggct atgagttctc gctctttttc ggaaacatct
    21181 tcaacatcat cgtagaagtg aggaatagcg atgtgtccct cttcatcttt aagctgtgca
    21241 agtatcttag tcagttcgag tatgggattg gcaaccgcgc ctccaaaaat gcctgaatgc
    21301 aaatcgcgat tagcagcagt gacttcaatt tcccagtaag ccaaaccgcg aagtccggta
    21361 gttatgctgg gtgtgctggc cgaaagcata cttgtatccg aaaccaaaat ggtatcacat
    21421 ttcagttttt cagtattggc gctgcaaaac tcttcaaggc ttggcgaacc aatttcctct
    21481 tcgccttcca gcagaaactt tacattagac ttcagttgat tggtttttac taaatattca
    21541 aatgcttttg cgtgcatgaa cgactgccct ttgtcatcgt cggctccgcg cgcataaatt
    21601 ttttcatctc tgatttcggg ttcaaatgga ggtgaagtcc ataattcaag aggttcggca
    21661 ggcatcacat cataatgtcc gtaaaccata attgtaggca attcgggatc aatcattttg
    21721 gatgcaaaaa caatcggatt acctgttgtc ggcagaattt ccgcgtaatc gactccagct
    21781 tcttcaagca aatgcttcca gcgttcagca cattgctgaa tgtcaggctg atggcttgta
    21841 tgagaactaa cacttgaaat tcgaatcaga ctgaaaagtt cctccaaaaa acgggattga
    21901 ttatcacgaa tgtaattttt gatattcata aatataaaaa ttaggttttt gagtttattg
    21961 atttgcaata tttattaatg cttcttttca gtatgtaaag ttaggaaaat ttgctaaaaa
    22021 acaaatttta tgatagaaat cgttcaatga ataacagcat ttatttttga attgagaaag
    22081 agataaaatt cacatttaat ttgctaaatg acaagttttt tgtaattttg catgtttaga
    22141 ttgtttcccg ggggtgataa gtcttttata tataattttc ttatacaaag aaacattaat
    22201 taattctatc agaaatcaga tttatgaaaa gatcatatat attatcaatt gcatcaattt
    22261 tatttttttt aactggctgt accactcaaa aagaacttgc atattttaat accattactt
    22321 ccggttcgac agagagtctg aatgcacaga tcaaaaatgc acgacctgaa ccaaaaattc
    22381 tggttgatga ccgtttgaca ataactgtat cagctgtaga tccaaacgcg gttgctgttt
    22441 ataatctgcc ttttgtatcc tatgcatctc cgggttcaga ccaaatttat tcatcacccg
    22501 ttttacaatc atatctcgta aaccccgatg gcaaaataaa tttccctgta ctgggcgaaa
    22561 ttaaacttga gggacttact ttacccgaag cttcatatct gattaaaatg aaactatcgg
    22621 agcacgttga aaatccaatt gtaaatatcc agtttgtgaa tttcagaatt actgttctgg
    22681 gtgaagttct tcgtcccgga cagtttacga ttccgaatga acgggtcacc ttactggatg
    22741 cattggggct ggctggcgat atgactgttt acggacgtag agataatgtg ctgattaccc
    22801 gaaacaacaa cggaaaactt gaatttgcca gaattaatct caattcagat gaaattttca
    22861 aatcaccata ctattatttg cagcagaatg atgtgattta tgttgaacca aacaccgtga
    22921 aatcaatatc atcacagaat attcctcttt atttatcttc attatccaca ttagccactt
    22981 tggtcgcact aatttattca atttcaaaaa aatagtattt tcgggattta acaacctgtt
    23041 tttacgaaat ttcaataata tggaaaaaaa caacaacata aataatactg agattattaa
    23101 tctgagggaa cttgtttcca aatacgttaa aaagtggtat tggtttgtgc tggcagtcat
    23161 atttgcattt atcattgcaa caatttattt aaagaaaact gataacaaat ttcaggttca
    23221 gactgtaatt ttacttcgga atgaagataa aaccagcgaa atttcccaac tggctatgat
    23281 ggaaagtttg gggctgatgg gaacatcccg ggtggttgat gatgaaattc aggtgataaa
    23341 ttccaataaa atcttaaatc aggtaattga caccctggga atacaagctg agtattttga
    23401 aaagaacgga ttgaagtacg ttgaaaaata taaatcttta cctttcgaac tgaatatacc
    23461 ggtgcatttt tccgactcgc tcaaagcacc tgtaagaatt gaagtaaaga cgggcactgg
    23521 gtcttgtaag ataaaggtaa aatatggaga agagttcagc aaaaattata cgctggataa
    23581 tatacagcaa ccgttctcaa cacctgcagg aacattgagt ttcagaataa attcgactgt
    23641 taaacctggg atgaagtaca aaatcaaact atatccaaag aaagagttgc tggcgcagtt
    23701 tcgttcaaaa ctgaatgtat cggtagtaaa taaacaatcc aatgctatca gaatatctat
    23761 cgtagagtcg aatatcaaga aggctgagga tattctgaac aaaatagtag agttatacaa
    23821 tctggacgct atcattgata aaaatatcgt agcaaccaat accggaaact tcatcgaaga
    23881 cagattagtg ctgataacaa aagaactttt tgatgtggag tcggaggttg aaaattacaa
    23941 aaaagccaat gcgctgaccg atattgcatc agaagcaggc atttatcttg aatccgccag
    24001 cgaatatgaa aaaaagattg ccgaacttga aactcagctc aatttaatac aatacattga
    24061 aagctatatc agggaaaata aaaatcagta taacctgata ccagccaata tcggaattga
    24121 ggataaatcg ctgatggaat taatcctcaa ttacaattca gtgttgctgg agaggatgaa
    24181 actatcgcgt actgccaatg aaaaaaaccc ggtgctgatg cagcttgagc aacaaataaa
    24241 agtgctgaaa ggtaatgtac ttgaaagcat tagcagtgta aaatcagggt tgaagatcgc
    24301 ccggaatgat gtgtcacgca aagatgctca gttcaattct agaattaaag ctgttccaac
    24361 tcaggagaga cagtttctgg agataaaacg tcagcaggaa atcaaacaga atttatatct
    24421 tttcctggct cagaaacgcg aggaaaatgc gttaagtctg gccagtacag caccggcagc
    24481 cagaactgtc gaccgggcat atgcttcatt gacgccagta tcgcctaaaa gaatgattgt
    24541 ttatctggta gctctgattt tgggactgct gattccgttc ttgtacattt acctgagaga
    24601 tctgatcaat aataaaattg aggatgttaa ggagtttcaa aaaaccgtta aaccaccatt
    24661 cctgggtagt atttgtatca gtcgcgaatc agacagggtg gctgtgcagg caggcaaaac
    24721 gtcaaccatc gttgagatgt tccggttgat acgtaccaat cttcagtttg tcatcggaag
    24781 taaaaaaaat ccggcaatcc ttgtcacctc cagtatcagc ggtgaaggaa aatcgtttat
    24841 atctataaat attgctatgt cactggcttt attgaacaaa aaagttgtac tgataggtat
    24901 ggatatccgt aatccggtgc tgaaggatta tatgcatttg tcgaatacca acggtcaggg
    24961 ggttacgctt tatctctcag attccagtgt tcagcttaga gatgtaatca ttccatccgg
    25021 ttataattca aatctggatg ttattccggc tggtcctgtt ccgcccaatc cttcggaatt
    25081 gctgttgagt tcccgactcg atgaactgat aatggaactc aagaaagtat atgattacat
    25141 cattctcgat tcagcaccga taggtattgt atcggataca tacttgctca acagggtaat
    25201 tgataactgc atctatgttg cccgtcagga tcatacaccg cgtgaagctt ccaacctgat
    25261 caatgaaata tatgcagaga aacggttgaa cggaatggca ctcgtactta acggcactcc
    25321 ggccacttct gcatatggat atggttacgg ttacggacgg tcaaagaata aattcaagaa
    25381 tatgcctaaa cttactttcg gggataaact gaatgaatca atcatgcgat tgttccataa
    25441 agactaaaca acaataattg tacagtataa aaaactctga gactattcag ggttttttta
    25501 ttgggtaatg ggaaacttat atattattgt agtttccttt tgttgttttg taatgaaatg
    25561 atatataatt aattatataa gatgttgttt tcttatgaaa aaaaatatct gatttattga
    25621 gcatcattac tgtttttttt atagttttgc aaattcaaat cataatatgg aaaaattagc
    25681 tgaaaatatt gtttataccg cacatcagaa atttataaat tttggtataa aaagtatctc
    25741 tgttgatgat atatgcaatg aactgcgtat atctaaaaaa actttttata ttcatttcgg
    25801 gcaaaaggaa tctctaatcg aagccgtatt ggaatatgaa atgagaaaaa acactgaggt
    25861 ttttgagaaa atgtataaaa acaagaatgc aattgatgcc ctgattataa tcatcaaaga
    25921 gcttaaaaac cgcatagata aagaaccgga tacatttctg cacgatctgg aaaaatatta
    25981 tccggtcact tacgcgaaat tcaaagacaa acagaccgtc aggatcagaa gttgtttcga
    26041 gaataatctg aaaaaaggga tagaagaggg attctacaga gaagacattg atatcgaact
    26101 cgtatccctg tttcactcca tacagatcag acacactttt gatgaaatgc agagttttac
    26161 acccaaaata ccgaagaaac gtctcgtgga tttttttata gatctgatcg taaaacttat
    26221 aaccaatgag aatggttaca ggtacatggt tgaaaactat tataaaattg ctgataatta
    26281 agaaaaataa aaagataatg aaaaagacag tattattcat tttcggattg atattcgcag
    26341 gtagtgtggc agcacaggat accatttccc tgagtttgcc acaggcaatc actatcgcgt
    26401 taagcgaaaa tcccactatc aagattgccg acaaagagat taaaagagtg gaatacaaca
    26461 agaaagaaaa atatggagca ttattgccca atgtttcttt aggattatcg tatgctagga
    26521 gtttgaaaaa acaaaaaatg tttttctcga tacccggtat gccttccaat cccgatggta
    26581 ttgaagtggg tcaggataac acgttcaatg gttccaccaa cggcttgatg gctacattgc
    26641 ctttatttgc tccggcactg tggcaatcaa tcaatatgtc ggaacttgat atggaacttg
    26701 cgcttgaaag tgcgcgttcc tcaaaaattg cactcgttaa tcaggtttcc aaagcctatt
    26761 atgccattct gatggcgcag gattcgtaca atgtactgaa aagaacctac gaaaactcaa
    26821 ctgaaaacaa ccggattgtt cagaataaat tcaaacaggg agtagtttcc gaatttgaat
    26881 ccatacgggc cgaagtacag ttacgcaatg tcggagccaa tctctcagct gccgaaaatg
    26941 gggttgaact gaccaggttg cagcttaaaa tgctgatggg agtggggatg gatcaggaaa
    27001 ttaaaatcga aggcagtttg gctgattacg aaaataagat gtactccgat gtgttgaaaa
    27061 ttgacacgac aacacttgcc gaaaattcgg acctgaaaca gtttgatatt aaatcacgtc
    27121 agcttaacca atcggttaaa cttcagcgag catcgtggat gcctacactt tcggccagtt
    27181 tcaactataa ttatatgtcg tatgccaacg acgatatcct cttcacaggt gatcagcgct
    27241 ggttccctac atcgaatgtg ggcattatgc tttcaattcc gctatttcag ggcggacagc
    27301 gttactttaa agacaaacaa ctgcaaatac aggttgatga actaaaagac caaaaactta
    27361 acctcaaacg cagtctggaa ctgcaggcaa tgagttacat aaataatatc gaaaaagcaa
    27421 tgaaactgat cgaatcaaac aaggttgctc tcagtcaagc cgaaaaagca atgactatat
    27481 cgcaaaaaag atacgaagtc ggagcaggaa cctatctgga tgtggcaaat gccgaactgg
    27541 cttatcagca atcgggcctg gcatacaatc aggctatata tgactaccta tcggcaaaaa
    27601 ccgacctgga aaaacttctc ggaaataaat aatataacgg taacaaaaaa catcataaaa
    27661 tatgaaaaac tataaattat taactatcag cttaatagca gttttttttg gctttatatc
    27721 atgtactaaa acaagcaaag acaaaacgca gcagaaagat gctgtattaa cggttaaatt
    27781 agcccaggtt tttgaacggg atgttcccca aaatcaggaa tatacggcta ctgttcagcc
    27841 tgaaattaaa aacaatattg ctccggcatc tccgggtcgt atccgccgta tattggtcaa
    27901 tgtgggaagc cgtgtagctg caggtcagcg tattgttcag atggatgctg caaatctggc
    27961 caattatcag acacagattg ataacctgcg ccgcaattat aatcgtattt ccgaattgtt
    28021 tgccgttggc ggttcatcgc aacaggaagt tgacaacgta aaggctcaac tcgaacaggc
    28081 tgaaaccacc cttcgtaatc tgaatgaaaa tacctatctg accagtccga taagcgggat
    28141 tgttaccgca aagaactacg acagtggcga tatgtattcg ggacaaatgc ctgtactgac
    28201 tgtgatgaaa ataaatcctg tgaaaatcct gataaatgtg tcggagtcgc attattcgaa
    28261 aataaaagcc ggtatgaacg tagatatcca tttcgatgtt tttccgaatc aatccttcag
    28321 tggcaacgta agccttattt atcccactat cgatgaacgt acgcgtactt tccctgtaga
    28381 aataaaactt gccaataatg acagtaaggt tcgtcccggt atgtttggac gcgtaactat
    28441 taattacgga accgaaaaaa gagttgtggt acccgatcag gctgttatca aacaggcagg
    28501 ttcaggagca cgctatgtat atacttacaa cgatggaaaa gtaagttatt tgcaggtaga
    28561 actcggacag cggctggaga atgagtacga acttatttcc ggagttccat cgggagcgca
    28621 ggtggtgata gccggacagg caaaactgac cgatggagca actgtaaagg tagtgaaata
    28681 atcctttttg aatgaaattg tttttttaca aaataattaa aaaatataat tgatgaaaat
    28741 atacgaaagt gctgtaaaaa agccgattat gacgatattg atttttatcg gagtaatgat
    28801 tttggggctt ttttcactga ataaattatc tatcgacttg ttacctgata tcgaaacaaa
    28861 tacgattatg gtaattacaa gttatcaggg cgcaagtgct gccgatatcg aaaccaatgt
    28921 taccaggcca ctggaaaata cgctgaatac tgtatccaat ctgaagaata tcacctcttc
    28981 gtcacgcgaa aacagatcaa tcataaccct cgagttcgat tacggtctgg atatcgatgt
    29041 gctgacaaat gatgttcgcg acaagctcga tatggttaaa acatttttgc ccgatgctgc
    29101 cggcgatccc atcattttca agtttagtac cgacatgatc ccggtaatga tgattgctgc
    29161 tactgctgat aaaagtttac cggcattgta caaaattctg gacgataatg tatctaatcc
    29221 actggcacgt atcaacggag taggttctgt ctccattagt ggtgcacccg aaagggaagt
    29281 gcaggtattt gttgatccgg ctaaaatgga ggcatataac ctgaccatcg agggtattgg
    29341 ccagaatata cggcttgaaa acgtgaatac accggtaggt aatatggata tcggaagcga
    29401 aacttattcc atccgggttg agggtgaatt cagcgatgtt tcccaattga aaagtattgt
    29461 cgtcggaacc tataatggta aaacagttta tttgtccgac attgccaatg tggttgacga
    29521 taaggaagaa cggttacagg aaagctatac caacggtgtt cagggcgcaa tgattgttgt
    29581 gcagaaacag gctggcgcca atacggttca gattgccaac gaaattaata aggttttacc
    29641 cgatattaag aagaatcttc cacccgatat aaaactcagt cttattaatg atacctcgca
    29701 gagtattaaa aacacgatta agggacttgc agaaactgtt ctgtttgcct ttctgttcgt
    29761 aattcttgtg gtattgtttt tcctgggccg ttggcgggct accatcatca tcatcctcac
    29821 cattccggtg tcgcttctgg cttcgttcat ttatttgtat gccacgggag gttcgctaaa
    29881 cgttatttcg ctgagttctt tatccatagc tatcggtatg gtggtggacg atgctattgt
    29941 tgtgctggag aatattacca cacatattga gcggggaagc agtccgaaga gtgctgccat
    30001 acatggaacc aacgaagtat ctttggctgt agttgcctct acgctcaccc tgatcgccgt
    30061 attctttccg ctgacgctta tttccggtat ggccggggtg atgttcgcgc agctcggatg
    30121 gattgttaca atcatcatga cgatgtcgct gatttgcgcc atgacactga caccgatgct
    30181 gagtgcacaa atgcttcagt acaacaaaaa ccacactccg tttttcaaaa aagtatttac
    30241 tcctatcgaa cgattccttg atggacttga tgacggttat gcccgtatgg taaactgggc
    30301 ggttcgtcac cgctggacag tgatggcgct tgctaccatt ttcttcctgc tttcagtgat
    30361 accgatggca tttgtcggat ccgacttttt ccctccgcag gacaatggcc gtattgccgt
    30421 caatattgaa atgcctgtag gtacacgcat ggagatttcg cgtgaagtgg cttcaaaact
    30481 tgaagctgaa tggagaaaaa aatatccttc ccttgaaatt gtcaactaca cggtagggca
    30541 agcaagcagt tcgaatatat ggggctccat gcagtcgaac ggaagtaaca tcgtgaatat
    30601 gaacctgggt atgataccgc ttaaagacag gagtttatcg atgtttagtc tttgtgacag
    30661 tctgcgccgc gatctggaag taatccccga aattcagaaa tcaaaagtta ttgccggagg
    30721 gggtatgaat atgatgggag gacaatccac gctcgacgtc gaaatatatg gctttgattt
    30781 ctcaaaaacg gataaaatag ctgccaattt gaaaactgaa ctgggtaaga ttgacggtct
    30841 ggcaaatatt aatatcagcc gtcaggatta tgtgcccgaa tatcaggttg atttcgaccg
    30901 cgaaaaactg gcgctgaatg gtcttaatat cagtacggct tccaattatc tgcgtaaccg
    30961 tatcaacggt ttgacagcct cgctgtaccg cgaagacggg gaagaataca atatccgtat
    31021 cgcctatgat ccgaagtatc gccagtccat tgaagccatc gaaaatatcc tcatatacaa
    31081 cgctcagggt caggcagtaa gactgaagga gcttggtaaa gtggtagaaa ggttcacacc
    31141 gccaactatt gagcgtaaaa accgtcagcg tgtcatcacc gttgcttcta cactctccgg
    31201 aaccactatg gataaagcaa ttaaatcaat caatgatgct attgctaaaa tggatatccc
    31261 tggcgatgtt taccttgaag taggagggtc gtaccaggat cagcaggaat cattcgccga
    31321 tatgggaact ctgctcttga ttatcgtact gctggtatat attgtaatgg catcgcagtt
    31381 cgaatccttg gtttatcctt ttattaccat gttttctatt cctttcggaa tatccggcgt
    31441 atcattagct ttattcatgt caggccagac actcaacatg atgtccgtta tcggagttat
    31501 catgctggtg ggtatcgtgg tgaaaaatgg tattgtgctt gtggactata tcaacctcaa
    31561 ccgcgaacgt ggtatgggta tcgttcaggc agtggttaaa ggcggacgct cccgtttgcg
    31621 ccctgtggtg atgaccacac tgaccaccat tctgggtatg ttgccaatgg ctttatccaa
    31681 cagtcaggga tccgaaatgt ggaaaccgat ggctgtgagt gttatcggag gtcttaccgt
    31741 atctaccttg ctgactttgg taattgtacc ttcgctgtat gccatcacag ccggtatggg
    31801 tatgaaacgg aaaatgcata agttggccaa agtaaataaa caatcagcac gatattgaaa
    31861 agctgtttta atcaattaat ttgaaaacaa aatgaaaacg atatttattc ctttcgatca
    31921 ggctttcaaa gatcaggtca ttgctttact gaacgaaaac catgtacgtg gtttcacttt
    31981 ctgggataat gtacaaggta ggggaagcaa aacaggcgaa cctcattacg gaaaccatgc
    32041 ctggccatca ttgaacaatg cattgctcac cgtggtagat gatgaaaaag taaaaccaat
    32101 tctggaggcg ctgaaaaacc tcgataaatc gtctgaaatg ctcggattaa gagcttttgt
    32161 atggaatata gaggaaatgt attaaacgcg cactgcacgt aaaaccattc cgaagctgtc
    32221 ttatacattg ttgtaaggca gcttttttta ttcagctgaa agggaataac tgaacagttg
    32281 atccggagtg agatgctcag aaaaacaaaa acgacctccc ccaaaaaatt ccccggtatt
    32341 tcaatctgtc gttatcggac ggaattcgga gttatcaatc tgcaaatgtc tcaaattcat
    32401 gcgaacgagg gaacgcatat agataattct aattgtttgc atcattacag ctgctgtaaa
    32461 tagtgatttt gggagaaata aatggatgct caaataaaat aggaatgtta ggcaatgtga
    32521 aaatgaatac ctaaacgtta tcaacaaaga ttaaaataat cttgaaaaat gttggatttt
    32581 taaattaaat ctgtaatttc gttggattgt tttttaaaaa atatgcatta ttggcagtta
    32641 ttttgtttaa tcggaattct gaacatgaca gaaatacaac tgttgtttca gttaaaactc
    32701 cattaaacaa gtgtttactc aaatttaatc aaagtaaaat aaaagaaaaa aatgatttca
    32761 gttattaatt tccagaatta gcaaatacgt cgataaaata aaggataagc ccgaaaatcc
    32821 tgaaaaaatg ggagagtaag ccgaaaagct aaagagtagg caaaaattaa aaaaataatt
    32881 atttatcact actgaccgac aaaaaacaag atttgtcggt tttaaatttt atatgatt
//
LOCUS       DAOPYB010000016        32246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1307_length_32246_cov_11.020969, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000016 DAOPYB010000000
VERSION     DAOPYB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 32246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32246
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1307_length_32246_cov_11.020969"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..1616
                     /locus_tag="PKH58_02475"
     CDS             363..1616
                     /locus_tag="PKH58_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNX87924.1"
                     /translation="MIYISNIIISLICLAFFSGMEVAFISSNKLRFELDKKYKNLTSS
                     IISIFYNNSQQFISTMLVGKFISIVIFGLLTTESLTFAFEGVMSLFISVLLQITIATV
                     VIIIVGEFLPRAFFKINPNMWMRLFSWVLLFFYIILFPVSWFSSWASSKFFGLFKISL
                     PPSTEENVFSRIDLNYLMQENLESKNDQKDMDNEVKIFQKALDFSAVKLRDCFIPRTE
                     IVALEFGVDVNTLKQTFIETGLSKIIIYKGDIDNIIGYIHSSEMFEHRKDWTKYIRQI
                     PIVPENMAAQKLMKIFMSEKKSIAVVVDEFGGTAGIVTLEDIMEEIFGDIEDEHDIRE
                     YTAEKTGENEYLFAGRLEVEEANKLFNLNIPVSDDYTTIAGYILHVHQHFPKLNEIIR
                     IENFTFKCVKVTNNRIEMVKLIVNN"
     gene            1767..3872
                     /locus_tag="PKH58_02480"
     CDS             1767..3872
                     /locus_tag="PKH58_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNX87925.1"
                     /translation="MATLNKIRSKGVLLAVIVGTALLAFIIGDFLNSGSTLFHQSKQN
                     VAEIAGEKINIAEFTSAIEQMTNVYKIETGQNEFNEEQTAQLRATVWDNLVNEKILAS
                     EAKKLGLSVSKEELTDRLIGKNIHPLIMQRRVFMDEKGQFSHENLLKFYNNIFEGTNA
                     DAEQLKEAKNYWLFWEKAVKNAILQEKYSMLFTKAVGSNSIEAKYNFEARRSTADINY
                     VMQPYFAVSDSSIQVSDAEIKERYNKEKELFKQEANRSINYVAFDVVPLNDDFKAAKE
                     WMNKVSEEFKTTSDVAAVVNSESDISYDGRNYSEKTVPANLKDFAFGNGTGAIFGPVF
                     QNNTYTMARIMEAGIMQSDSVKLRHIFLAGNDEKKADSIIGAIKSGSKFADMAMKYSV
                     VKETAANGGEIGWITEGAVAKDITGPAFSKATNEIFKVSSAQGIQIMQIMEKTPARRK
                     VKLAILERKVTPSSQSYSKIYNEAKQFAASSTDMEKFEKNAKQKGYVINPATGLLKNT
                     DRINAVPQSRQVVRWAFNNKIGNVSDVFDCGTQFIVATTSEINEEGYKTVAQATQQLK
                     TEIIKDKKADMMIKNLSGLVAKYPTLETLAAALNTQVKTAPAVNFASYQFGEAGFEPY
                     IIGKSSVLTSGKISAPLKGNSGVYVLLPGVKQVDNSPFNPKMETAQMDARMAYSLPYT
                     LMEKLKEKYNIVDNRSNFY"
     gene            3983..5023
                     /gene="rlmN"
                     /locus_tag="PKH58_02485"
     CDS             3983..5023
                     /gene="rlmN"
                     /locus_tag="PKH58_02485"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNX87926.1"
                     /translation="MKKQPLIGKSHNELKEIVAGLSMPSFTAKQITEWIYKKKIGSID
                     EMTNISAKNREILAEKYEVGCNKPIQVVESSDGTKKYLFQTEVGHYIETVYIPEDDRA
                     TLCVSIQVGCKMNCLFCMTGKQGFNGHLSPTEIINQIISIPETDKLTNIVFMGMGEPF
                     DNMMSLLQSLEIITSSYGFGWSPRRVTVSSIGIVPGMKVFLEKSDCHLAISLHSPFHE
                     ERERLMPMEKVYPIEKVIAELKKYDFSHQRRLSFEYIMFEGTNDSIRHAVELVKLLKG
                     LDCRLNLIRFHEIPGIDLRSSNPDKMEFFRNYLNNNGIITTIRRSRGEDIQAACGLLS
                     TKEKLQHNANSL"
     gene            5050..8037
                     /locus_tag="PKH58_02490"
     CDS             5050..8037
                     /locus_tag="PKH58_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HNX87927.1"
                     /translation="MKITKNIILIIACISLLYSFAPAHSTFNTPSPTTSLPKDTIVEI
                     TVVSDTTDTQTVVIDTITTKEKDLEEIDTTLIQTEKPPLSILKDKFSIPTVTRPLTRN
                     LSIPFTPSKGLDKRHIALWQSHGLYYMQSKEKWNWQRVNLFETREDLYTQSYVLPFLV
                     PMLENAGATVLIPRERDTRTTELIVDNDKSSVDCSFRTTTGVFHWHQGVGSGFANYKD
                     TYRDGENPFKSGTFIQANTVGNKDNPSIAEWAYSVPETGCYAVYVSYKTLPNSCSEAI
                     YTVYHAGGETKFGVNQTMGGGTWIYLGNFVFEKKKPQHSRVVLTNQSSDINKLVTADA
                     VKIGGGMGNISRKRSDLITKKIIKVPIKSKRKKRKKRYKTKTITTVKQFDNYSTSGMP
                     RFTEGARYWLQWAGAPDSVYSRTSGANDYSDDFQSRGFWVNYLAGGSASFPGGKGLNI
                     PVDLAFALHTDAGKTSDSIIGTLAIHTVKTKTGAVKYNNGISRWVGRNLTDSVQTQVV
                     NDIQQLHDKDWVRRQLWNRSYSESRVPEVPAMLLELLSHQNFPDMRYGLDPRFRFTAS
                     RAMYKGILRYLANANNFEYKVQPLPVEAFYAEFSDSTEVELHWKPVEDPLEPTAKPTG
                     YIVYTRVDDGGFDNGEYAGSNCFRKKIERNKIYSFKVEAVNEGGRSFPSEILSVCKTS
                     DDNNTVLIVNAFNRISAPASFDYGDVSGFNNSWDGGVPYINDYKFTGIQTEFRKNRPY
                     RSDENPGHGASETGWEGKVIAGNTFDYPYLHGKSIMKAGYSFISSSEKAVADGDVKLY
                     CYPYVDMIMGKEREWTSLNKENPEPVFRTFTPEIQKRIIEYTDSGGNLLVSGSNIISD
                     LVLSKNKTSDDRSFVEKTLKIKWMVDKVNDVDKVSFNNSGNKNFHGIDLSFYVAPNEQ
                     SYYVEAPDIILPNDEYATQICEYVINKQGAGMVYDGNYRICALGFPFETIRQEHEREK
                     LMKYILDFFSDNNRNLIPKIKTK"
     gene            8039..9112
                     /gene="pdxA"
                     /locus_tag="PKH58_02495"
     CDS             8039..9112
                     /gene="pdxA"
                     /locus_tag="PKH58_02495"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNX87928.1"
                     /translation="MESEKIIIGITHGDINGIGYEVILKALSDNRILDMFVPVIYGSS
                     KLAGFHKKNLELQSLNLNVINSLDEVNTKRINIINCVDDELKVELGQSTPEAGKAAFE
                     ALEKATEDLKNGKLHAIVTAPINKKNIQSADFSFPGHTEYLENKFGEKSSSLMMLVND
                     HLRVAVVTGHIPISEVAGAITKDKIVSKLRILNNSLKQDFSIIKPRIAVLSLNPHAGD
                     EGVIGNEEQNVIIPALKQTDTEGILCFGPYPADGFFGSDNFHDFDAILAMYHDQGLIP
                     FKALSMENGVNFTAGLSVIRTSPAHGTAYNLAGQNKASEQSFLSALYMACDIYRNREN
                     FSQMKANPLIVEVKKESYKRNIE"
     gene            complement(9209..11032)
                     /locus_tag="PKH58_02500"
     CDS             complement(9209..11032)
                     /locus_tag="PKH58_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNX87929.1"
                     /translation="MDALDTNIEMDDLNEGLKQPEVIHEPSTEAIMEANPEDAVTHEL
                     TKQQICDRMKELNELDVETAKEEVESIKQMFYKKSKSETAEQRVAFLEKGGNEADFIP
                     EPTELENEFKELLNEFRNKKAKLNALQEQEKENNLLKKQHIIEQMKQLVESNDDVSVN
                     VAAFRQLQQEWKNAGPVPPTATNELWKQYNLYQESFWDLIKINNELREYDFKKNLEAK
                     QKLCEAAEQLADEEDVVTAFRQLQKLHENWHELGPVARELREEIWNRFKAASTVVNKK
                     HQSHFDDIRKVEDENMSQKVSLCEKIEAIDATKFTSYKEWDEATKIILEMQEEWRSVG
                     FAPRKSNQKIFERYRKACDKFFTAKAEFYKEVKSGLSQNLEQKKLLCEKAETLKDSTD
                     WKEAGEQLVQLQKEWKTIGPVAKKYSDEIWKRFITACDYFFEQKNKNVSGQRSVEQQN
                     LDKKKELITKIASFEKTANVSESLAALRALAAEWNAIGHVPFREKDKIYKEYRTMLDA
                     QFDKLNIDASQRRLESFRNNLKDMTTKGDNKLYREREKLVRAYEHLKSEIATYENNIG
                     FLSIASKKGDGMLKEMERKIETLKDECKLLEKKITLIDDQM"
     gene            complement(11192..11953)
                     /locus_tag="PKH58_02505"
     CDS             complement(11192..11953)
                     /locus_tag="PKH58_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX87930.1"
                     /translation="MNMTALVTGATSGFGRAIALRLAKLGYNLIVTGRRKELLDTLCD
                     EIRKNYSTNVIALNFDVRDQKACEEAVNSLSDEFKNIDLLVNNAGLAAGAASFDESLL
                     EDFERMIDTNVKGLLYMTKLIVPGMIARQTGHIVNLSSIAGIEVYPTGNVYCASKHAV
                     NAITKGLRIDLVKHGIRVSSISPGMAETEFSIVRYHGDSEKAKAVYNGLTPLNAEDIA
                     DALEFIVTRPAHVSINDIQINPAQQANTYISHRIQ"
     gene            complement(11950..13176)
                     /locus_tag="PKH58_02510"
     CDS             complement(11950..13176)
                     /locus_tag="PKH58_02510"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNX87931.1"
                     /translation="MEKKLNSIEDAIKDFKNGKIVIVVDDEDRENEGDFICAAEKITP
                     EQVNFMLKHGRGVLCAPLIEERCDELELNMQVEVNTSIHSTPFTISVDKLEGCTTGVS
                     ASDRAATFRALADPTSVPDTFGRPGHIFPLRARSKGVLRRAGHTEAAVDLARLSGLNP
                     VGALIEIMNEDGSMARLPQLYDIAEEFNLKIISIEDLIAYRIRTESLIDKGEEVDMPT
                     AFGHFKLIPFRQISNGLEHIALIKGTWEKDESVLVRVHSSCMTGDIFGSMRCECGEQL
                     HKAMELIEKEGKGAIVYMNQEGRGIGLMNKIKAYKLQEEGMDTVDANLHLGFHADERE
                     YGVGAQILREIGLTKIRLMTNNPKKRIGLEAYGLSIVENVPLEVKPNQYNAFYMQTKK
                     EKMGHKLKFVGKENQK"
     gene            complement(13190..14683)
                     /locus_tag="PKH58_02515"
     CDS             complement(13190..14683)
                     /locus_tag="PKH58_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNX87932.1"
                     /translation="MLGIKKLDIYVIKSFLAYFFMTFFICILILMMQFTWKHLAELVG
                     KGVEWKVLAEFFWYTTLQTVPMALPLAILLAALMNFGNLGENFELIAMKSAGISLFRI
                     MRGLIVFIAFICVAAFFFSNNVLPIAQNKLWTLIFSLRQKSPEFEIPEGEFYSEINGI
                     NLYVRHKVPEKKLLKDLMIYDFSNGFDNATVTLADSGRVQFTADNKYLKMTLLSGESF
                     ENLKQPGNSSATNIPYRRETFSTKEMLIDFDTEFNRFDESILKDQHISKNIVQLTHTI
                     DSVSKIVKARGNEQAVDMIKVQFANFQVNDSSGIKKQNAKINPDSLFMSLAQEKMEEA
                     MRVSQENLRAAREKIDFNKVMLEEPTYYVRRHQVEWHRKFTLSFACLIFFFIGAPLGA
                     IIRKGGLGLPVVISVLMFIFYYIIETSGYKMAREGFWEVYQGMWLSSAVLLPIGLFLT
                     YKAVTDASLFRSEQYVKILEKTVDFLTNFWRKKKNRINTGMTEISAQ"
     gene            complement(14753..16129)
                     /locus_tag="PKH58_02520"
     CDS             complement(14753..16129)
                     /locus_tag="PKH58_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX87933.1"
                     /translation="MNNVAESNLGTMNIRKLLWMYSLPAIVGTMISSMYNIIDRMFIG
                     QGVGPLAISGLALTFPFMNFLAAFAMLVGAGAASRISIRLGEGDKDRAEKILANALML
                     TFIISGVVIIISRVFMNPILVLFGGSENTLEYARQFMRIIIPGSILSALNFGFNNIMR
                     ASGYPKKAMLTMIICSGINVCLAPLFIFGFHWGIAGAAHATNISYFVGSVWVLTHFMQ
                     KKSIIRFHKRNFHLNKQIIKSILNIGMSPFSMMIASSMVIVLLNIALLRHGGDLAVGA
                     LGIQNSIVTLFLMFIIGLNQGIQPILGYNYGAQNYDRMFSTLTRAALIATIVTTIGFL
                     LSTFFSPVLVSIFTTDEELKSISANALRISVIMLPLVGSQIVFTSFFQSIGKAKISML
                     LSLARQVLFLIPAVLILPAFFGLNGVWFSMPVADSLAVVVTSITIFTFIRKFEKQTSY
                     KFRLKSVR"
     gene            complement(16234..16908)
                     /locus_tag="PKH58_02525"
     CDS             complement(16234..16908)
                     /locus_tag="PKH58_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX87934.1"
                     /translation="MKFRLLAILFVLFSLTVVELQAQSTKTDTLETKKLDYRFEAGYA
                     HNYRYGDYVSSTPYHVIRMGVNAQYPLKHGFGIETGLKYSYMFGKKQQIYTIGDSVNY
                     SYTGHSIDVPVRLIYTLPIFWGLKVFGYAGPVFNIGLAQSSNVTVYGLPVVEGFPIDI
                     ATGTYNLYKTELNRFSVMLGAGGGVQWKQYRVKGGYDWGMNSISKDKSSPQRLRGWYA
                     AFEYDF"
     gene            complement(17117..17767)
                     /locus_tag="PKH58_02530"
     CDS             complement(17117..17767)
                     /locus_tag="PKH58_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX87935.1"
                     /translation="MKKNGFILIALLALICQPAFTQKKIDIGVQGGYNYSMPKLKSSR
                     LYGNLNGFHVGPVLTYNIKEQIGVQTGILYNYYNTVVSKITVGTSGTWKQNRIIGQGI
                     DIPLRAIYSLPLADDLIVQFGAGPNFNYALSKLQQKENYVDSKIVSSLTDKGTSIYAS
                     PSDFNRLDTQIGLSLGIKYTGISLRASYDLGFFDRDKTSSGSYKENNIKISLGYTF"
     gene            complement(17919..18539)
                     /locus_tag="PKH58_02535"
     CDS             complement(17919..18539)
                     /locus_tag="PKH58_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX87936.1"
                     /translation="MKTIKLSLVAFATFFVIGANAQVGLQAGYSNSKETGSGLSLNGF
                     HIGPAAEMTIQGPISLQYALLYNYLTRSNEVSILEQSVKTTTTAHTLDLPFRVAANFP
                     MNNGIGVFIFGGPNFSYALSQVTKASTSGLIENELNTENIYKLELSNGKKAYSPFDLQ
                     LGIGGGIKYKSISLSASYDWGMLDRDNSDNGVWKNNDLKISLGYNF"
     gene            complement(18796..19965)
                     /locus_tag="PKH58_02540"
     CDS             complement(18796..19965)
                     /locus_tag="PKH58_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX87937.1"
                     /translation="MKKLFLIVMIIASAITYTSAQTAPATDAAHWSFALKGGLDYFNI
                     HPVGDDIVDNGSWGAGAAIEYTINPIVGVGLNLDYLNFNRSTIKGKTIDPSFFTSVNL
                     SNLLFPKRNSAKLNFYANFGGGVAIPSYSDEVLSTSTYRRGVSMMPYKTNALAYTGAA
                     MEYNVSKLIGIGLEYTYRGYIAPDNAYVEYNDANALMATLRFKLGTGSKTHVRDMTTL
                     EYYPAPAPVIKQVENPYDDSAIKSRLDGLDKAGMDTQNRLKALEDDVKGLKDQAEGTK
                     VSASFQNIEFDFNSDKITKASYPTLDQVASILKSNATWGKLMINGHTDNVGSDAYNQK
                     LSEARAAAVKAYLAGKGLPEAAMSTAGYGESKPIADNATAAGRQNNRRVEFEISK"
     gene            complement(20181..20578)
                     /gene="ssrA"
                     /locus_tag="PKH58_02545"
     tmRNA           complement(20181..20578)
                     /gene="ssrA"
                     /locus_tag="PKH58_02545"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(20720..22240)
                     /gene="rny"
                     /locus_tag="PKH58_02550"
     CDS             complement(20720..22240)
                     /gene="rny"
                     /locus_tag="PKH58_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNX87938.1"
                     /translation="MEYILIAIVALVVGAFGSYLLLGVFNKRARREMEAEAELMKKNK
                     MIEAKERFIALKAEHEQQVQQRNAKVQAAEVKIQQREMQLNQRQSELQRKLNETEAQK
                     EGLEQQLNALEIKKRETEHLNRLAQEQLETISGLSADQAKERLVESLKEEAKTNALSY
                     INDIMDEAKMTANKEAKKVVIQTIQRVATETAIENSVTVFHIDSDEVKGRIIGREGRN
                     IRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLSLHQLVTDGRIHPARIEEVVNK
                     VRKQVEEEIIETGKRTTIDLGIHGLHPELIRMVGKMKYRSSYGQNLLQHSREVANLCA
                     TMASELGLNPKKAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKFKEKPEIFNAIGAH
                     HDEVEMETMLAPIVQVCDAISGARPGARREIVEAYIKRLNDLENLALSYPGVIKTYAI
                     QAGRELRVIVGADKLDDKETELLSFDIAKKIQDEMTYPGQVKITVIRETRAVSYAK"
     gene            complement(22380..22673)
                     /locus_tag="PKH58_02555"
     CDS             complement(22380..22673)
                     /locus_tag="PKH58_02555"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNX87939.1"
                     /translation="MAEEDMFNINITVNGVRMPLHISREDEVIYRNAEKLFNRFFRQY
                     SEVYSQRSMEEILTLVAYQFAYIVSKQGMNQDVGPLAEKIQQLNKQLKELMPE"
     gene            complement(22679..22972)
                     /locus_tag="PKH58_02560"
     CDS             complement(22679..22972)
                     /locus_tag="PKH58_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87940.1"
                     /translation="MENRYASLISEFELRIKKLISEYKALSKENETLKAELGRKQEDL
                     MTAHKEILDLRNDYKILQTTMGLGGSEEERRNSRQHLDKIVREIDKCLMLLND"
     gene            complement(23095..24759)
                     /gene="sulP"
                     /locus_tag="PKH58_02565"
     CDS             complement(23095..24759)
                     /gene="sulP"
                     /locus_tag="PKH58_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HNX87941.1"
                     /translation="MKFFDPKIVYTLKHYSKKQFYSDLMAGIIVGIVALPLAIAFGIA
                     SGVTPEKGLYTAIIAGFIVSLLGGSRVQIGGPTGAFIVIVYGVVQQYGITGLAIATMI
                     AGLMLIAMGVMKLGTIIKFIPYPVVVGFTSGIALTIFATQIKDLFGLQIEKVPADFIH
                     KWVVYAQNITTINLWATLIGILSIVIIAITPKFSKKIPGSLIAIILMTIVVYVLKHNF
                     GVQGIETIGDRFSINAALPQASKFSIDFDTVRTLLPVAFTIAMLGAIESLLSATVADG
                     VIGSRHNSNTELIAQGVANVITPIFGGIPATGAIARTMTNINNGGRTPIAGIIHAFVL
                     LLILLFLGNLTKHIPMACLAGVLVIVAYNMSEWRTFKSLLRNTKSDVSVLLATFFLTV
                     IFDLTIAIEVGLLLAVVLFIHRISETSTISVLESEVEAADYVEGAVDTDKLLIPKGVE
                     VYEIEGPFFFGLANKFEEIMKQMSDKPKVRIIRMRKVPFIDSTGVHNLENLIKMCKKD
                     KTQVLLSGVNDNVRKVLLQVELDKKIGNENICSNINEALERATKLI"
     gene            24992..26776
                     /gene="ispG"
                     /locus_tag="PKH58_02570"
     CDS             24992..26776
                     /gene="ispG"
                     /locus_tag="PKH58_02570"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNX87942.1"
                     /translation="MWESDPYNYHRRVTDEVKAGTLKIGGENPVRVQSMANTNTNDIE
                     SSTAQCLRIVEAGGELVRFTTQGLKEVENLNIIHAKIREADCHVPLVADVHFNANVAD
                     MAARYVEKVRINPGNYVSGIKTTLTSDYTEAEYQEEFEKIRTRFKAFLDICNTYGTAI
                     RIGVNHGSLSERMLNRWGNTPEGMVESCMEFLRICREENFTDVVISMKASNTVLMIQS
                     VRLLVETMEKENLRFPVHLGVTEAGDGEDGRIKSAAGIGGLLAEGIGDTIRVSLSEEP
                     EAEIPVTKLLRDYITQRESHEAIRLNPEYKISPLQLSNRKSNAVKNIGGENVPVVISR
                     PCNHQNLKADYILLDEEIQNTSGEKFGVYADKAPEVKVSELSFLELNYSRLDYKTLEY
                     LKTNPQIVILLSTDHINPPGEQRAFIHTLIYEGCNNPVIVVGNYRENDLESLQIKAAA
                     DLGILFVDGLADGILIKNEGRIPENEILSISFGILQAARVRVSKTEFISCPGCGRTMF
                     NLQTVVAEIKTKTSHLTGLKIGIMGCIVNGIGEMADADYGYVGAGRGKVSLYKKQTCI
                     EKNVPESEAVEHLINLIKSYGDWKEPEL"
     gene            26867..27328
                     /locus_tag="PKH58_02575"
     CDS             26867..27328
                     /locus_tag="PKH58_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87943.1"
                     /translation="MKKLFIAIILLTVLTNLNIAQATDKAIGIRGLSNSAEISFQNSL
                     SKSTRLELDLGLYGLDDRNGFILTGIHQWVFGLDKGLNWYVGLGGQLGSRWYEKNNLW
                     QSGFGLALAGQIGIEYNFTIPVQVSLDYRPAWYLTHGGTYDTVALGIRYRF"
     gene            27431..27886
                     /locus_tag="PKH58_02580"
     CDS             27431..27886
                     /locus_tag="PKH58_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87944.1"
                     /translation="MKKLFLAIALLIASSSIIQAQTMDKAIGIRITDGAELSFQNPLG
                     SSTRAEVDLGIYGNNSVILTGIHQWVFGLQEGVNWYAGLGPQIGLWDKLYSSGKHLAL
                     GVAGQVGIEYNFSVPVQVSLDYRPGFTIFPSGHDYNSFWAIALGIRYRF"
     gene            complement(27962..29917)
                     /locus_tag="PKH58_02585"
     CDS             complement(27962..29917)
                     /locus_tag="PKH58_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNX87945.1"
                     /translation="MKKLIFIKSALVVLLLCAPLLSAFAIRAYPGIVKITQPDGSSLN
                     IRMHGDEYHHYTTTEDNYIVKKIDSGFYTYASIDSRGKIIAGTQIARDPSVRQAADNK
                     YLVRMPEKLTTKFQSGIRKVKSTKQNAAVMKRAFPVTGSGKSLIILVNFKDKSFVVPS
                     IQTAFTNLLNQPGYSENNGTGSAKDYFMACSYGKFSPQFDVVGPFDLPSNMSVYGGND
                     EDGYDINPVQMIVDACTAADNNGVDFAQYDTDNDGYVDNIFVYYAGYNEAELGGDDTV
                     WPHRWGIYPTVTYGDEGNYAGTVASITFDGKKIMDYACTSELKGDSDSKMCGIGTFCH
                     EFGHVLGLPDYYDTSLNQDNTLNNWSIMDQGAYLNQGNTPPVYSAYDRFYLGYYKPVE
                     LNQPANLVTLLPLYQGTTIPANTDNQAYLLSAITHDLNGADPKPAEFFMVEYRKKTGW
                     DAYLPGEGMLIWHIDYDQTAWENNGPNNYNESTQTQASHMRVYLQPLSGSTTTPGDAF
                     TSGSFTPTAWDGTSIDRNITEIAKTAENVTFKLMGGLQPKIKLGMISGQLTYPLTVVG
                     KTNPKNLNIVTTGINGNLTVTLSGANADYFTISTASIPQTTANSTSGFDLLVTYKPLT
                     AGTHTAVLTITGGGLNDKVINLTGSAK"
     gene            complement(30034..30363)
                     /locus_tag="PKH58_02590"
     CDS             complement(30034..30363)
                     /locus_tag="PKH58_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87946.1"
                     /translation="MEEKSSYRQKVDAQFEDLKTDLEDLKIKVQNAAADLQAEYQEKI
                     RELEPKIVEGKVRLAELAGTADEAWDEVKDGAELILKQMKETFNNVKEKFNKDEASEN
                     PDVPKNE"
     gene            complement(30462..31409)
                     /gene="corA"
                     /locus_tag="PKH58_02595"
     CDS             complement(30462..31409)
                     /gene="corA"
                     /locus_tag="PKH58_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNX87947.1"
                     /translation="MSELRLFYHEDGKIKISKSLDILPKIDLKNIIWIDLNDVALDVE
                     SRLEQFLKIYIQEDEEIEEIEMSSRYIETDDGIVANTNFMMDNFTLEPVSFILKNGIL
                     VSVRDADLRSFNETVKKIFASTRSFPTGYHVLVALLETRVEYDADVIEDTTDLITNLS
                     KTMNKEDDLDEDILIRIKDLQEKVMIIRQTIIDKQRVISSMLKSDVFPAELLPRLSMI
                     IKDINSLFEYTRFGFDRLDYLQDTFLGLVNLQQNKIIKIFTVVSVIFLPPTLIASIYG
                     MNFKVLPELNWAYGYPFSLFLMVAFSGAVLWYFRRKKWL"
     gene            complement(31528..>32246)
                     /gene="rsmA"
                     /locus_tag="PKH58_02600"
     CDS             complement(31528..>32246)
                     /gene="rsmA"
                     /locus_tag="PKH58_02600"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNX87948.1"
                     /translation="IAESLPLSQAMPVLEVGPGMGVLTQYLLQNPLVDLTAVEIDRES
                     VDYLHLHYPQLKVIEGDFLKMDLSALYPDKFCIIGNFPYNISSQIFFKVLDYRDRIPY
                     VSGMIQKEVAERIASAPGKKAYGILSVLMQAFYTTDYLFTVSEQVFDPPPKVKSAVIR
                     FTRNEVQHLDCDEKLFRTVVKTAFNQRRKTMRNSLKPLVPKESSMYVDPIFDKRPEQL
                     GVAEFVQLTQMVEKALKENQ"
BASE COUNT         9624 a         6705 c         6229 g         9688 t
ORIGIN      
        1 aggaccccat ccttaaaagg gatgtgctct acctgctgag ctactagctc atttttctca
       61 aaaagcggat gcaaagatac gtcttttttt tggaagtaca aaccatactg tctttttttt
      121 agcaatttat attctacacc ccacctgaac gcatcagaaa atacgttttt tcatgaatga
      181 cagcttcaga aaattgtcgg tacatgatat caacgaattc actgactgta atcgctttaa
      241 tttttgccgg tatattgttg aaaaaaccta aattattgta tctttgtaac ctgattatct
      301 gagaaaaaat tgtctgaaaa caaattttcc ggatttgaat tattcaatta aatcccttgc
      361 aattgattta tataagtaat ataattattt ctcttatttg ccttgccttc ttctccggga
      421 tggaggtggc atttatttca tccaataagc tgagatttga acttgacaag aagtataaaa
      481 atcttacatc atccatcatt tccatctttt ataataattc gcaacagttc atttccacca
      541 tgcttgttgg gaaattcatc agcatcgtta tttttggttt actgaccacc gaatcgctta
      601 cctttgcttt cgaaggggtc atgagcctgt ttataagcgt attattgcaa ataactatag
      661 ccactgtcgt tattataatc gtgggtgaat tcctgccgcg agcatttttc aaaattaatc
      721 cgaatatgtg gatgcggtta ttttcgtggg ttctcttgtt tttctacatt attctctttc
      781 cggtttcctg gttcagttcc tgggcttcga gcaagttttt tggtcttttt aaaatcagtt
      841 taccaccttc gaccgaagaa aacgtttttt cacgcattga tctgaactac ctgatgcagg
      901 aaaacctcga atcgaaaaat gaccagaagg atatggataa cgaggtaaag atttttcaga
      961 aagcgctgga tttttcggct gtaaaattgc gtgactgctt cataccgcgt actgaaatag
     1021 ttgctctgga gtttggtgtg gatgtcaata cgctgaaaca aacatttatc gaaaccggct
     1081 tatcaaaaat tatcatatac aaaggcgata tcgacaacat tatcggctat attcattcat
     1141 ccgaaatgtt tgagcaccgc aaggactgga cgaaatacat ccgtcagatt ccgattgtgc
     1201 ccgaaaatat ggccgctcag aagctgatga agattttcat gagtgaaaag aaaagcatcg
     1261 ctgttgtggt cgacgaattc gggggaacag caggcattgt tacactcgaa gatattatgg
     1321 aagaaatttt cggcgatatt gaagacgaac atgacatcag agaatataca gctgaaaaaa
     1381 caggagaaaa tgaatatctg tttgcaggca ggctggaggt ggaagaggca aacaaacttt
     1441 tcaacctgaa tattcctgtt tccgatgatt atacgacaat tgccggatac attctgcatg
     1501 ttcatcaaca ttttcctaaa ctaaatgaaa taatccggat tgagaatttt accttcaaat
     1561 gtgtaaaagt gaccaacaac cgtatagaaa tggtaaaact gatagtaaac aactaaaaca
     1621 aaccggatat aaaacactgt taatcaaaac gtaatttaaa taatactatt aaaaagcacg
     1681 gtaatactca ttttttttgt atattcgtgc cctcaatttt taagtaataa acacaaccta
     1741 aaatatttat caatttatta atagaaatgg caacattaaa caagatcaga agtaagggag
     1801 tattgcttgc agtaattgta ggaactgcat tattagcatt tattatcggt gactttttga
     1861 attcaggttc tacacttttt catcagtcca aacaaaatgt ggctgaaata gccggtgaaa
     1921 aaatcaatat cgctgaattt acgtcagcca tcgaacagat gactaacgtg tataaaatcg
     1981 aaaccggaca aaacgaattt aacgaagaac aaacagcaca gttaagggct acggtgtggg
     2041 ataatctggt aaatgaaaaa attcttgctt cggaagccaa aaaacttgga ttgtcagtaa
     2101 gtaaagaaga gctgacagat cgtctgattg gaaagaatat tcacccgctc atcatgcagc
     2161 gccgtgtttt catggacgaa aaaggacaat tcagccacga aaatctactc aaattctaca
     2221 ataacatctt tgaaggcaca aatgcagatg cagaacaact caaagaagct aaaaattact
     2281 ggctgttttg ggaaaaagct gttaaaaatg ccatactaca ggaaaaatat tccatgctgt
     2341 ttacgaaagc tgtaggttcc aacagcatcg aagcaaaata caactttgaa gcccgcagat
     2401 ctactgctga cataaattat gtcatgcaac cttactttgc tgtatccgat tcatccatcc
     2461 aggtttctga tgctgagatt aaagaaagat ataacaaaga aaaagaacta tttaaacagg
     2521 aagctaaccg ttctatcaac tatgttgctt ttgacgtagt tcccctgaac gatgatttca
     2581 aagctgccaa ggaatggatg aataaagtta gtgaagaatt caaaaccacc tcagatgttg
     2641 ccgccgtggt taactccgaa tcagacatta gctacgacgg acgtaactat tccgaaaaaa
     2701 ccgtacctgc aaacctgaaa gattttgcat tcggcaatgg aaccggcgct attttcggcc
     2761 cagttttcca gaacaacacc tatacaatgg cacgtatcat ggaagcaggt atcatgcaat
     2821 ccgactctgt caaactgcgt catatcttcc tggctggcaa cgacgagaaa aaagctgaca
     2881 gcatcatcgg agctattaaa tccggttcaa agttcgccga tatggcaatg aaatattcag
     2941 tagtaaaaga aactgccgct aacggtggtg aaattggctg gatcactgaa ggcgctgttg
     3001 caaaagatat tacaggtcct gctttctcga aagctaccaa cgaaatattt aaagttagct
     3061 cagcacaggg catccagatc atgcaaatta tggaaaaaac tcctgcacgc cgcaaagtaa
     3121 aactcgctat cctggaacgc aaggtgaccc caagcagtca gtcgtacagt aaaatttaca
     3181 atgaagccaa acagttcgct gcaagcagta cagatatgga gaaatttgaa aagaacgcaa
     3241 aacaaaaagg atatgtaatc aatcctgcaa ccgggttgct taaaaatacc gaccgtatta
     3301 acgcagttcc tcaatcacgt caggtagtac gctgggcctt taataataaa ataggtaatg
     3361 tttcggatgt atttgattgc ggaacacaat ttatcgtggc aacaacttcg gaaattaatg
     3421 aagaaggtta taaaacggta gcgcaggcaa cccaacagct gaaaacagag attatcaagg
     3481 ataaaaaagc tgacatgatg attaaaaatc tgagcggctt agttgccaaa tatcctactc
     3541 ttgaaactct ggctgcagcg ctgaatactc aggttaaaac tgcccctgct gtaaactttg
     3601 ccagctatca gtttggtgaa gctggtttcg agccatacat catcggaaaa tcttctgtac
     3661 ttacttccgg taaaatatca gcaccgctca aaggtaactc cggtgtttat gtccttctgc
     3721 ccggtgtaaa acaagttgac aattctccgt ttaatccaaa aatggaaaca gctcagatgg
     3781 atgcccgcat ggcttattca ttgccttata cactgatgga gaaactgaaa gaaaaatata
     3841 acatcgttga caaccgctcc aatttttatt aataaaacac ggtaaatttc ataatacatt
     3901 ataaaacgga atggatataa ttcattccgt ttttattttt cgtcgaatta tcgtattttt
     3961 gtgaaagatc agacattaaa caatgaaaaa acagccactt atcggaaaat cgcacaatga
     4021 actgaaagaa atcgttgcag gattatctat gccgtcattc accgccaaac aaatcacaga
     4081 atggatttac aaaaagaaaa tcggttctat tgacgaaatg acaaatattt ctgcaaaaaa
     4141 cagagaaata ctggctgaaa aatacgaggt aggctgcaat aaacccattc aggtagtcga
     4201 atcttcggac ggaacaaaga aatacctctt tcaaaccgaa gtagggcatt acatcgaaac
     4261 cgtatatata ccggaggatg atcgcgcgac attgtgtgta tccattcagg ttggatgtaa
     4321 aatgaactgt ttgttctgca tgacaggcaa acagggattt aatggtcacc ttagtccgac
     4381 agaaatcatt aatcagataa tttcaatccc cgagactgac aaactgacga atattgtatt
     4441 catgggtatg ggcgaacctt ttgacaacat gatgtcgctg ctgcaatcgc tcgagataat
     4501 tacctcatcc tatggatttg gatggagtcc cagaagagtt accgtttcat ccatcggaat
     4561 cgttccgggt atgaaggtgt ttctcgaaaa gtcagattgt catctcgcaa tcagtctgca
     4621 ttctcctttt cacgaagaac gtgagagact gatgcccatg gaaaaggtct atcccattga
     4681 aaaagtgatt gcggaactca aaaaatatga tttcagccat cagcgcagac tttcatttga
     4741 gtatatcatg ttcgaaggaa caaatgacag catccgccac gccgtggagc tggtaaaact
     4801 gcttaaagga cttgattgca ggcttaatct tattcgtttt cacgaaattc caggcattga
     4861 cctgagaagt tcgaatccgg ataaaatgga attctttaga aattatctca ataacaatgg
     4921 aataatcacc accatccgtc gttcacgggg tgaggatatt caggctgcct gcggtttgct
     4981 gtcgaccaaa gaaaaattgc aacacaacgc aaattctctg tgatttatcc tctgtgatcc
     5041 tgtttcaaaa tgaaaattac aaaaaacata atattaatta tagcctgtat ttctttgctc
     5101 tacagcttcg ctcccgctca ttcaacattc aacacacctt ctccaaccac atcattgccg
     5161 aaagatacaa tagtagaaat aactgttgta agcgacacga cagacacaca aactgtagtc
     5221 attgatacca ttaccaccaa agaaaaggat ttggaagaaa ttgacactac tctcattcaa
     5281 actgaaaaac caccgttaag cattctaaag gataaatttt ccatcccaac tgtcacacga
     5341 ccgttgacaa gaaatctatc tattcccttt accccttcca aaggactgga caaaagacac
     5401 atcgcgcttt ggcagagcca tgggttgtat tacatgcaat cgaaggaaaa atggaattgg
     5461 cagcgtgtca acttatttga aacccgtgaa gatttataca cccaatcata cgtactccct
     5521 tttcttgtcc ccatgcttga aaatgccgga gctacagtac ttataccccg cgaaagggat
     5581 acccggacaa ctgagttaat tgttgataat gataaaagct ccgttgattg cagcttcagg
     5641 acaacaacag gtgtttttca ctggcatcag ggagttggca gtggttttgc caattacaaa
     5701 gacacctatc gcgatggcga aaatcctttc aaaagcggca catttattca ggcaaatacc
     5761 gtcggcaata aagataatcc cagcatagcc gaatgggctt attcagtacc ggaaacagga
     5821 tgttacgccg tttacgtttc gtataaaaca ctgcctaaca gttgcagtga agccatatat
     5881 accgtatatc acgctggtgg tgaaacaaaa ttcggagtta atcaaactat gggcggaggt
     5941 acctggattt atctgggaaa ctttgtgttt gagaagaaga aaccgcagca ttccagagtt
     6001 gtactgacca accaaagttc cgatatcaac aaacttgtta ctgccgacgc tgtaaaaatt
     6061 gggggaggta tgggtaatat cagccggaaa agatctgatc tgattacgaa aaagattatt
     6121 aaagtaccga ttaagagtaa aagaaaaaaa agaaaaaaaa gatataaaac aaaaacgata
     6181 accaccgtta aacagttcga caactacagc accagcggta tgccacgatt taccgaagga
     6241 gcaagatact ggcttcagtg ggcaggtgcg ccggattcgg tgtacagccg gacgagtgga
     6301 gcgaatgatt attccgacga tttccaatcg cggggtttct gggtaaacta tctggcaggc
     6361 ggttctgcat catttcctgg aggcaaagga ctgaacatac ctgtcgacct tgctttcgct
     6421 ttgcacaccg atgccggtaa aacctcagat tctatcattg gtacacttgc catacataca
     6481 gtgaaaacca aaacaggtgc cgtaaaatat aacaacggca tttcacgctg ggttggccgc
     6541 aacctgactg acagtgtaca aacacaggtt gtcaacgata ttcaacagtt gcatgataag
     6601 gactgggtaa gaagacaact ctggaaccgg agttacagcg agtcccgggt gccggaagta
     6661 cctgccatgc ttctcgaact tctttcgcat cagaacttcc cggatatgcg ttatggcctg
     6721 gacccgcgtt tccgcttcac tgcaagccgt gcgatgtata aaggcatact tcgttatctg
     6781 gcaaacgcca ataattttga atataaggtt cagcctttgc cggtagaagc tttttacgcc
     6841 gagttttccg attcaacgga agttgagttg cactggaaac cggtggaaga tcccctggaa
     6901 cctacggcga aacccaccgg atatattgtt tataccagag ttgacgatgg aggtttcgat
     6961 aacggagagt atgccggttc caattgcttc cgaaaaaaaa ttgaaagaaa taaaatctat
     7021 agttttaagg tcgaagcagt taatgagggt gggagaagct tcccgtcgga aatactgtcg
     7081 gtatgtaaaa ccagcgacga caacaatacc gttcttatag tcaacgcgtt caaccggatt
     7141 tctgcaccag cgagtttcga ctatggagat gtatcgggct tcaacaatag ttgggacgga
     7201 ggtgtgcctt atatcaatga ttataagttt acgggtattc agactgagtt cagaaaaaac
     7261 cggccttaca gaagcgatga aaatcccggt cacggagcga gcgaaacagg ctgggaagga
     7321 aaagtaattg ccggaaatac attcgactat ccttatctgc atggcaaaag catcatgaaa
     7381 gcgggttatt cttttatttc gagcagtgaa aaagctgttg ctgatggcga tgttaagctt
     7441 tattgttacc catacgttga tatgataatg ggtaaggaac gtgagtggac gagtctgaac
     7501 aaagaaaacc cggaacctgt attcaggacc tttactcccg aaattcagaa aagaattatc
     7561 gaatataccg actcgggagg gaatttgctg gtaagcggat ctaatatcat ttcagatttg
     7621 gttttatcga aaaacaaaac ttccgacgac aggtcgttcg tggaaaaaac tttgaaaatt
     7681 aaatggatgg ttgataaagt aaacgatgtt gataaagtca gttttaataa ttcagggaat
     7741 aaaaatttcc acggaattga cttatcattt tatgtagcac ccaacgaaca gtcctattat
     7801 gtggaagcac ccgacatcat actccctaac gatgaatatg ccactcagat ttgcgaatat
     7861 gtaataaata agcaaggtgc agggatggta tacgatggga attacagaat ttgcgcgctt
     7921 ggcttcccgt tcgaaaccat cagacaggag cacgaacgcg aaaaactgat gaaatacata
     7981 ctggatttct tttcagacaa caacagaaat ttaattccta aaataaagac taaataatat
     8041 ggaatcagaa aaaattatta ttgggataac acatggtgat atcaatggca tcggctacga
     8101 agtaatcctg aaagccctat cggacaaccg catactcgat atgtttgttc ctgtgatata
     8161 cggatcttca aaacttgccg gatttcataa aaaaaacctc gaattacaga gcctcaatct
     8221 gaatgtgatc aattctctgg atgaggtaaa caccaaaagg ataaacatca tcaactgtgt
     8281 ggatgatgaa ctgaaggtcg agctgggaca atccaccccc gaagcaggaa aagcagcatt
     8341 cgaggcactg gaaaaagcta ccgaagatct gaaaaacggc aaactgcatg caattgtcac
     8401 cgcgcctatc aacaagaaaa acattcagtc agccgatttc agtttccccg gccatacaga
     8461 atatcttgag aataaattcg gagagaaatc atcttccctg atgatgctgg ttaatgacca
     8521 tttacgtgta gctgtggtaa ccggtcacat accgataagt gaagttgcag gtgcaattac
     8581 aaaagataaa attgtcagca aactccgtat ccttaacaat tcattgaagc aggatttttc
     8641 aatcattaaa cccaggatag ctgttctgag cctgaacccg catgccggcg acgaaggagt
     8701 aatcggcaac gaagaacaga acgtaattat tcccgcgctt aaacaaaccg acacggaagg
     8761 cattctctgt tttggcccct accctgccga tggattcttt ggctccgata acttccatga
     8821 ttttgatgca attctcgcta tgtaccacga ccagggactg atcccattca aagctttatc
     8881 catggagaat ggcgtaaact ttacagccgg attatctgta atacgaacct cacctgcaca
     8941 tggcactgct tacaatttag ccggacaaaa caaagcatct gaacaatcgt ttctgagtgc
     9001 cttgtatatg gcttgcgata tttaccgcaa cagggagaat ttcagccaga tgaaagcaaa
     9061 tccgctcatc gttgaagtta aaaaggaaag ctacaaaagg aatattgaat aattctgttc
     9121 gcggattatt aactacccta ttaaaaaaca aaagcaccgg aattccggtg cttttgtttt
     9181 tgttttataa cctgttttgc tttgtaattt acatctgatc atcaataagc gttattttct
     9241 tttccagaag cttacattca tctttaagcg tttctatctt gcgttccatt tctttgagca
     9301 taccgtcacc ctttttggat gcaatgctaa ggaaaccgat attgttttcg taagtagcaa
     9361 tctctgactt aagatgttcg tacgcccgaa ccagtttttc ccgttcgcgg tacaacttgt
     9421 tatcgccttt ggtagtcata tccttcaggt tattgcggaa cgattccaga cggcgctgtg
     9481 aagcatcaat attcagttta tcaaactgag catcgagcat tgtgcgatat tctttgtaga
     9541 ttttatcctt ttcgcggaag ggaacatgac ctatggcatt ccattcagca gccaaagcac
     9601 gtaaagcagc aagcgattcg gaaacgttcg ctgttttttc gaaacttgca atttttgtaa
     9661 tcagttcctt tttcttatcc agattttgct gttccacact ccgttgtccc gatacattct
     9721 tattcttctg ttcgaaaaaa taatcgcagg ctgtaataaa gcgtttccag atttcgtctg
     9781 agtatttttt tgcaaccggc ccgatggttt tccattcttt ctgaagctgt acaagttgtt
     9841 ctccggcttc cttccagtca gtgctgtctt tgagagtttc agccttttcg caaagcagtt
     9901 tcttctgttc caggttttgc gacagaccgg attttacttc tttataaaat tcagcctttg
     9961 cagtaaagaa tttatcacat gccttacggt aacgttcgaa tatcttttgg ttcgattttc
    10021 gcggtgcaaa acctaccgaa cgccattctt cctgcatttc aagaataatt ttggtagctt
    10081 cgtcccactc tttatatgat gtgaattttg tagcatcaat agcttcaatt ttctcgcaga
    10141 gagatacttt ctggctcata ttttcgtctt ccacttttct gatatcatca aagtgcgact
    10201 gatgtttttt atttaccact gttgaagcgg ctttgaatcg gttccagatt tcttcacgca
    10261 gttcgcgggc aacagggccc agctcatgcc agttttcgtg cagtttttgc aactgtctga
    10321 aagcagttac gacatcttct tcatcggcaa gctgttcggc tgcttcgcaa agtttctgtt
    10381 ttgcttcaag atttttcttg aaatcatatt cgcgaagctc attattaatt tttatcaaat
    10441 cccagaacga ttcctgatat aaattgtatt gtttccagag ctcgttagtg gccgtgggtg
    10501 gtactggacc tgcatttttc cattcttgtt gcaattggcg gaaggcagct acgttaaccg
    10561 agacatcatc attgctttca acaagttgtt tcatctgttc gatgatatgc tgttttttca
    10621 gcagattgtt ttctttttcc tgctcttgca gagcattcag ttttgccttc ttatttctga
    10681 attcattcag cagttctttg aattcatttt caagttctgt tggttcgggt atgaaatccg
    10741 cttcatttcc tcccttttca agaaaagcca cacgttgttc agcagtttct gatttgcttt
    10801 ttttatagaa catttgtttg atagactcaa cttcttcctt cgccgtctca acatccaact
    10861 cattcaactc tttcatgcga tcacaaatct gctgtttcgt cagttcatga gtgacagcat
    10921 cttcaggatt tgcttccatt attgcttcag ttgatggttc gtggattacc tcgggctgtt
    10981 tcagcccttc atttaagtca tccatttcaa tattagtgtc aagtgcgtcc ataataagtt
    11041 ttttttagta tgttaaagag ttaagtcttt agcattaatg tccaattcgt tcagaaaaaa
    11101 atatgcaact acaaaaataa gataattttt caaataattt aatccttttg ttacgaaatc
    11161 tttaaaactt cgacatttgg ttatataccg gttattgtat tctgtgcgat atataagtat
    11221 ttgcctgctg agcaggattt atttgtatgt cattaatact gacatgcgcc ggacgtgtaa
    11281 cgataaattc gagggcatcg gctatatctt ccgcattcag aggagtaagt ccgttgtaaa
    11341 ctgcttttgc cttttcagag tctccatgat atctgacgat ggaaaattcg gtttcggcca
    11401 tgcctggtga tattgagcta acacgaatgc catgttttac cagatcaatc cgaagtccct
    11461 tggtaatggc gttcacggca tgctttgacg cgcaataaac attgcctgtc ggatatactt
    11521 cgataccggc tatggatgat aagtttacaa tgtgacctgt ttgacgggca atcattccgg
    11581 gtacaatcag ttttgtcatg tacaacagtc cctttacatt tgtatcaatc attctttcaa
    11641 aatcttccag cagactttcg tcaaaggagg cagcaccggc cgcaagtccg gcgttattta
    11701 caagcaggtc tatgttttta aactcatccg aaagcgaatt cacggcttct tcacaggctt
    11761 tctggtcgcg aacatcaaag tttaatgcga tcacgttagt ggaataattt ttgcggatct
    11821 cgtcacaaag cgtgtcaaga agctccttac gcctaccggt aactatcagg ttgtaaccca
    11881 gtttcgcaag ccggagcgca atggcgcgtc caaatccgga agttgctccg gtaactaatg
    11941 ctgtcatatt catttctgat tttcttttcc aacaaatttc agtttatgac ccatcttttc
    12001 ctttttggtc tgcatataaa atgcattata ttgattgggt tttacctcaa gcggtacatt
    12061 ttcaacaatg gaaagaccgt aagcctcaag accaattctc tttttcgggt tgtttgtcat
    12121 cagtctgatt ttggtcagac caatttcgcg aagtatctgt gcgccaaccc catactcacg
    12181 ttcatcggca tgaaaaccga gatgtaaatt ggcatcgaca gtatccatgc cttcttcctg
    12241 gagtttatag gctttgatct tgttcatcaa accaatgccg cgtccttcct gattcatgta
    12301 aacgatagcg ccctttcctt ccttttctat cagttccatg gccttatgga gctgttcacc
    12361 gcactcacaa cgcattgaac caaaaatatc accggtcatg caggatgaat gtactcttac
    12421 cagtacggat tcatcttttt cccacgtacc ttttataagc gcgatatgtt ccagtccatt
    12481 ggatatctga cggaagggta ttaacttgaa atgtccgaac gcggttggca tatccacttc
    12541 ttcaccttta tcgataagcg attctgtgcg gatgcgatag gctattaaat cttcaattga
    12601 aataatcttc aggttaaatt cttcggcaat atcgtaaagc tgcggaagcc gcgccataga
    12661 tccgtcttcg ttcattattt caatgagcgc gcctacagga ttaagtcccg acagacgtgc
    12721 aaggtcaact gccgcttcgg tatgtccggc acggcgcaga acgccttttg aacgggcacg
    12781 cagtggaaaa atatgtcccg gtcgtccgaa agtatccgga actgatgtcg gatcagctaa
    12841 agctctgaaa gttgcagcac gatcggaagc cgaaactccg gtagtacatc cttcgagttt
    12901 atcaacactg atagtaaacg gagtggaatg gattgaggta ttcacttcaa cctgcatatt
    12961 cagttccagt tcgtcgcaac gttcttcaat caggggagca cacaggacac cacgtccgtg
    13021 tttcagcata aaattaacct gctcgggagt tattttttca gccgcacaga taaaatcacc
    13081 ctcgttttcg cggtcttcat catccaccac aatcacaatt tttccgtttt tgaaatcttt
    13141 gattgcgtct tctattgaat tgagtttttt ttccatagtt agaaattaat tattgtgctg
    13201 aaatttcagt cattccggtg ttaatcctgt ttttcttttt tctccagaaa tttgttaaaa
    13261 aatcaactgt tttttccaaa atcttcacat attgttccga acggaaaagc gatgcgtcag
    13321 taacagcttt gtaagtcaga aacaaaccaa taggcagaag tacagccgag cttaaccaca
    13381 taccctgata tacttcccaa aatccttcgc gagccatttt atatcctgaa gtctcgataa
    13441 tataataaaa tatgaacatc aaaacagaaa tcactaccgg caatcccaat ccgcccttgc
    13501 ggatgatcgc gccaagcgga gctccgataa agaagaaaat cagacaggca aatgacagag
    13561 taaactttct atgccattct acctgatgac gacgtacata ataagtgggt tcttccaaca
    13621 tcactttgtt aaagtctatt ttttctcttg cagctctcaa attttcctga gaaacccgca
    13681 tagcttcttc cattttctcc tgtgcaagcg acataaataa agaatcggga ttgatttttg
    13741 cattctgttt ttttatgccc gaagagtcgt ttacctgaaa attggcaaac tgaactttta
    13801 tcatatcaac tgcctgttcg ttgcccctgg ctttaacaat tttggacacc gagtctattg
    13861 tatgagtaag ctgaactata tttttactga tatgctgatc tttcagaatt gattcatcga
    13921 aacggttaaa ttcggtatcg aagtcgatta gcatttcttt tgtgctgaaa gtctcccgtc
    13981 tgtatggtat attagtggct gatgaatttc ccggctgctt aaggttttca aagctttcgc
    14041 cactaagcag cgtcattttc aggtatttat tatcggcggt aaactgtacc cggccagaat
    14101 cagccagcgt cactgtcgca ttgtcaaatc cattggagaa atcatatatc atcagatctt
    14161 tcagcaattt tttttcaggc accttatgtc tcacatatag attaatccca ttaatttcgg
    14221 aataaaattc accttcgggt atttcaaact caggtgattt ttgcctcagt gaaaatatca
    14281 gcgtccataa tttattctgg gcaataggca acacattgtt cgaaaagaaa aatgcagcca
    14341 cacagataaa ggcaatgaaa acaatcagac cgcgcatgat ccggaataac gatattccgg
    14401 ctgatttcat ggctatcagt tcaaagtttt ctccgagatt tccaaaattc atgagtgcag
    14461 ccagaagaat agctaacggc aatgccatgg gtactgtctg tagcgtagtg taccagaaga
    14521 attcagctaa aactttccat tctacgcctt tacctaccag ttcagccaga tgtttccagg
    14581 tgaactgcat cataagtata aggatacaaa tgaaaaatgt catgaaaaaa taagccagaa
    14641 aggactttat tacgtatatg tctaactttt taattccaag catttattta atctttttga
    14701 gtgaacaaaa gtacttaatt tttagtatac aacgaattca tttctgataa aattacctaa
    14761 cgctttttaa cctgaactta tacgaagttt gtttctcaaa ctttctgata aaggtaaata
    14821 tggtaatgct ggttacaaca acagccagac tgtcggcaac aggcatcgaa aaccagactc
    14881 cgtttagtcc gaaaaatgcc ggcaatatta aaactgccgg aattaagaaa agcacctgtc
    14941 tggccagact tagcaacatg gatatttttg ctttcccgat tgactgaaaa aaactggtaa
    15001 atacaatttg agaaccaaca agcggtaaca tgataacaga tatccggagg gcatttgctg
    15061 atatgctttt cagttcttca tcggttgtaa atatacttac caataccggc gaaaaaaatg
    15121 tagaaagcaa aaaaccaatg gttgtaacaa tagttgcaat aagagcagct ctggtaagag
    15181 tgctgaacat tctgtcataa ttctgcgctc cgtaattata ccccagtata ggttgaatac
    15241 cctgattaag accaataata aacatcagga acagggtaac aatactgttc tgaataccaa
    15301 gcgctccgac tgccagatcg ccaccgtgtc ttagtaatgc tatattcaac agtacgatta
    15361 ccatacttga ggcaatcatc atactaaaag gcgacatacc gatattcagt atagacttaa
    15421 taatctgttt attcagatga aagtttcttt tgtgaaaacg tattattgat ttcttctgca
    15481 taaaatgggt cagcacccat acagatccca caaaatatga aatatttgtt gcgtgagcag
    15541 cgccggcaat accccagtga aaaccaaaaa taaataatgg tgccagacaa acatttatcc
    15601 ctgaacagat tatcattgtc agcattgctt ttttcggata acctgaggca cgcataatgt
    15661 tgttgaatcc aaaattcaaa gctgataaaa tgctgcctgg aataataata cgcataaatt
    15721 gcctggcata ctccagtgta ttttcactac ctccgaaaag tacgagtatc gggttcatga
    15781 atactcttga aattattatt acgacccctg aaataataaa tgtaagcatt aatgcatttg
    15841 caagtatttt ttccgcacga tctttatcgc cttcacccaa ccggatggat attctggaag
    15901 cagcccctgc tccgacaagc atagcaaaag ccgccagaaa attcataaac ggaaatgtca
    15961 gcgcaagacc cgaaatggcc agcggaccca ccccctgtcc tataaacata cgatcgatga
    16021 tattatacat agaggatatc atcgtaccaa caattgccgg caacgaatac atccacaaca
    16081 atttgcggat attcatcgtt cccagattac tttcagcaac attattcatc ccaataaatc
    16141 ttattttttt cagaaattaa aacatagaca gcctaatcaa aaaaaaacgg ctttacagag
    16201 taaaaccggt tttttgtatc aatttattct tagttagaaa tcgtactcaa aagcagcata
    16261 ccatcctctc aaccgttgtg gtgaactttt atctttgctg atactgttca tcccccaatc
    16321 ataaccacct ttcacccggt attgcttcca ttgcacacca ccaccggcac caagcattac
    16381 tgagaaacgg ttaagttctg ttttatacaa attatatgta cctgtagcaa tatcaatcgg
    16441 gaaaccttcc actaccggta atccataaac tgttacattg gatgactgag ctaatccgat
    16501 attgaataca ggacctgcat atccgaagac tttcagtccc cagaatattg gtaatgtata
    16561 aatcaatctt accggaacat caatggagtg gcctgtgtat gaataattta cactatctcc
    16621 aatggtatat atttgttgtt tttttccaaa catgtacgaa tacttcaatc ccgtttcaat
    16681 tccaaaacca tgcttgaggg gatactgagc attcactccc atccgaatta catgataggg
    16741 tgtagaacta acataatcac cataccgata gttgtgagca tatcctgctt caaaccggta
    16801 atcaagcttt ttggtttcca gggtatcggt tttcgtcgat tgtgcctgga gttcaacgac
    16861 tgtcaatgaa aataaaacga aaagaatagc taatagtctg aatttcattt gaaatatatt
    16921 tctttttaaa tgattactga taatactaat tgcttatttc ccggttggtt tttttcggta
    16981 aaattttatc tatgtttttg caaaaatagt gataaatacc tacatacgct acaacacaaa
    17041 acgtttatta acacaaaaag gacaagcatc gattagcctg tcctttgccg gataaaacaa
    17101 aaattctatc tgattatcaa aatgtatatc ctaatgatat cttaatattg ttttccttgt
    17161 atgatccact gcttgttttg tccctgtcaa aaaaacccag gtcataactt gcccgaagtg
    17221 aaattcccgt gtacttaatg cccagactta aacctatttg tgtatcaagg cggttgaaat
    17281 cggaaggtga agcataaatg ctggttccct tatctgtcag actgctgaca attttactgt
    17341 caacataatt ttctttttgc tgaagcttac tcaatgcata gttgaaattt ggtccggctc
    17401 cgaattggac tattaaatcg tcggctaaag gcaatgaata aatcgcgcgc aacggaatat
    17461 caattccctg acctattata cggttttgtt tccatgttcc acttgtacca actgtaattt
    17521 tagaaacaac tgtattgtaa taattataca atattccggt ctgaacgcca atttgctcct
    17581 tgatattata ggttagtacc ggtccgacat gaaatccgtt gagatttccg tataatcgag
    17641 aactctttaa tttaggcatg gaataattat atccaccctg aacgccgatg tcaattttct
    17701 tttgggtaaa agccggctga caaatcaggg ctaatagtgc tatcagaata aatccgtttt
    17761 tcttcatgtt gtatgtgtta aaattataga tgcaaagata aacaaacctt tgagctttga
    17821 gaaatttaat tccgtttttt ccttaaacag aataagttta gttcaaaaaa aaacagccag
    17881 ttttttgact gactgttcaa ttaaaatatt tttatatttt aaaaattgta tcctaatgag
    17941 attttcaggt cattgttttt ccaaacccca ttatcggagt tatcacggtc taacataccc
    18001 caatcgtaac ttgcactgag tgagattgat ttatatttaa ttccgccgcc aatacctaac
    18061 tgcaaatcaa atggtgaata agcttttttt ccattgctca gttctaattt atagatattt
    18121 tctgtgttta attcgttttc aataagcccc gaggtggatg ctttagtcac ctgagataac
    18181 gcataactaa aattaggacc tccaaagata aaaacaccaa taccattgtt cattggaaag
    18241 ttagcggcaa ctctaaaggg taaatcaagg gtatgtgcgg ttgtagtagt tttcacagat
    18301 tgttccagaa tactgacttc gttcgatctt gtcaaataat tatacaataa tgcatattgc
    18361 agactgattg gtccttgaat tgtcatttcg gctgcaggtc ctatatgaaa accatttagt
    18421 gatagtccgc taccggtttc tttagaatta ctgtaccctg cttgcagacc tacctgtgcg
    18481 tttgcaccaa ttacaaaaaa tgtagcaaat gctacgagtg ataatttaat tgttttcatt
    18541 ttttctaatc ttgtttaaat ttaaaaatca tgttttactt tttcacttca ttttttaaaa
    18601 tctacttccg gaaatgcaat tttcatgcca tagctccata atttagcgta taaatttttc
    18661 aatagttgca aaatacacat taccggattg aattttttat taatatttca gaaatttcgg
    18721 atgatgcctg tttcttttta aaagcaaatg aggatacgcc agtggcatat cctcattctt
    18781 atagactaag ttctcttatt ttgaaatttc gaattctacg cgacgattgt tttgacgacc
    18841 ggctgcagta gcattgtcag cgataggttt gctttcaccg taacctgctg ttgacatagc
    18901 tgcttcagga agtcccttac ctgcaagata agcttttact gctgctgcac gagcttctga
    18961 aagtttttga ttgtaagcgt ctgaaccaac gttatcagtg tgaccgttga tcatcagttt
    19021 accccatgta gcattagatt tgagaataga agctacctga tccaatgtag gataagaagc
    19081 ttttgtaatt ttatctgagt taaaatcaaa ttcaatattc tggaaagatg cacttacttt
    19141 tgttccttca gcctgatctt taagaccttt aacgtcatct tcaagagctt tcagacgatt
    19201 ctgagtatcc atgcctgctt tatcgagtcc atcaagacgg cttttgatag cggagtcatc
    19261 gtaaggattt tccacttgtt tgatcacagg agctggagcc ggataatatt caagtgtagt
    19321 catgtcgcgg acatgtgttt tgcttccggt accaagtttg aagcgcaatg tagccatcaa
    19381 agcgttagca tcattgtatt ctacatatgc gttatcagga gcaatgtaac cacgatatgt
    19441 gtattcaagt ccgataccaa tcagtttgct tacattgtat tccatggcag ctccggtata
    19501 agccaatgca ttggttttat aaggcatcat tgaaacgcct cttctatagg tacttgttga
    19561 taatacttca tcagaatatg aaggaatagc aacaccacca ccaaaatttg cgtagaagtt
    19621 caattttgca ctattacgtt ttggaaacaa cagattggaa agattcactg aagtaaagaa
    19681 acttggatcg attgtttttc ctttgatggt tgaacgattg aagtttaaat aatccagatt
    19741 aagtcctact ccaacgatag ggttaattgt gtattcaatt gctgcaccgg caccccagct
    19801 gccattgtca acgatatcat caccaaccgg atgaatgttg aaataatcga ggcctccttt
    19861 aagtgcaaac gaccaatgag cagcatctgt agcaggtgct gtttgagcag atgtgtacgt
    19921 aatagcacta gctattatca ttacgattaa gaatagtttt ttcatcattt tgttttttat
    19981 tagcagttaa aataaatttt acttatatta tttatttata atttacaaaa atacatttaa
    20041 tttttaacta aagtgctttt ttgattaaaa attttattat aaaatgaaat tattgttcag
    20101 atttaactaa actgatactt tcacagatat cgttttcatc aaaaaaaact ttcttcgtgt
    20161 ttattacatc aaaaaagtaa aagtggagct ggagggagtc gaaccctcgt ccaaacaagg
    20221 aaaatatatg ctttctacat gtttatcttc gctttaattt tcgtacaatg gcaagaccaa
    20281 agccaccaac cattatctta tcctttgttg tttcatcgtc cttacaaggc taagggcgac
    20341 tatccccgat ttagctgcac ctctgtatca aatcgcctcg gagcgacggc atttgagaga
    20401 tgtcccgtcc tggcacctgg tgcctggatg aagctaaatc tactttattc gattaagcag
    20461 caagagcgta attgttttcg ccagttagaa tgttgaaatc cgggattaac gagccggcat
    20521 tccgacgctc gacatgctta catatctttt ctgcctgctg tcaaaaccaa gcagccccaa
    20581 aaaactcttt ttttgttcgt tgtaaattta acaatttccg tgccaaaact tcaaagatta
    20641 taacaatctt ttaaaaaata tgttcagtaa ataataataa atatacaata tcctgaaaat
    20701 gtgctgataa aatcaaccgt tattttgcat aactaacggc acgcgtttcg cggataacgg
    20761 taatttttac ctgtccggga tatgtcatct catcctgaat cttcttagca atatcaaatg
    20821 acagaagttc tgtttcttta tcgtcgagtt tatcggcacc tacaatcacg cgcaattcac
    20881 gtccggcctg aatggcatag gttttaatta cacccgggta agataatgcc agattttcca
    20941 ggtcattgag acgtttaatg tatgcctcta ctatttcgcg ccttgcaccc ggacgtgcac
    21001 ccgatattgc atcgcacacc tgtacaatgg gcgcaagcat cgtttccatt tccacttcat
    21061 cgtggtgcgc tccgatggcg ttgaaaattt cgggtttttc cttaaatttt tctgccagtt
    21121 tcatacccag aatagcatgt ggcaattccg gctcatcgtc aggtactttt ccaatatcat
    21181 ggagcagtcc ggcacgtttg gctttcttcg gatttaatcc cagttcggag gccattgttg
    21241 cacaaagatt ggcaacttcg cgcgagtgtt gcaacaaatt ctggccatat gacgagcgat
    21301 atttcatttt tccaaccata cggataagct ccggatgtag tccgtgaata cctaagtcga
    21361 tagtagttcg ttttccggtt tctatgattt cttcttcgac ctgtttgcgg actttattaa
    21421 cgacttcttc tattcgggca ggatgaattc gtccgtcagt tacaagttga tgtaatgaca
    21481 gacgtgctat ttcgcggcga accggatcaa atgccgaaag tactatagct tcgggagtat
    21541 cgtcaacaat gatttcgaca cctgtagctg cttcgagcgc acgtatgttt ctgccttccc
    21601 gaccaataat acggcctttc acttcgtcgg aatcgatatg aaatacggtt accgagtttt
    21661 caatcgccgt ttccgtagct acccgctgaa tggtttgaat aaccaccttt ttggcttctt
    21721 tatttgctgt cattttagct tcatccatga tatcattgat atatgacaaa gcattggttt
    21781 ttgcctcttc cttcaatgat tccaccagac gctcttttgc ctggtctgca gacaaaccgg
    21841 aaattgtctc cagctgttcc tgggccagac gattaagatg ttcagtttca cgctttttga
    21901 tttccaaagc attcaactgc tgttcaagac cttctttttg tgcttcggtt tcattcagtt
    21961 ttcgctgcaa ttcactctga cgctgattga gctgcatttc gcgttgctga attttaacct
    22021 cggcagcctg aacttttgca tttcgttgct gaacctgttg ttcatgttct gctttcagag
    22081 ctataaagcg ttctttagct tcaatcattt tatttttctt catcaattcg gcttcagctt
    22141 ccatttcacg gcgggctctt ttattgaata ccccaagaag caggtaactg ccaaaagctc
    22201 caacaacaag cgcgactatg gcaataagta tatattccat gttgtaaaat tattaattgt
    22261 gtatatatat ttaaataaaa aaacgcatta aactgcaaac ttaccatccg acggaaaacc
    22321 ggacaacaaa taaacaatta atgcggtaaa ataaatgttt tcagtctgat aatgccgttt
    22381 tactctggca tcaattcctt caattgtttg ttgagctgtt gaattttctc ggccaacggt
    22441 ccaacatcct gattcatgcc ttgttttgaa acaatataag caaactgata agccactaac
    22501 gtcaaaattt cctccatgct ccgctggctg tacacttcac tatattgcct gaaaaacctg
    22561 ttaaaaagtt tttcggcatt tctatatatc acttcatcct cccgactgat gtgtaaaggc
    22621 attctaaccc cgttgacagt aatatttata ttaaacatat cttcctctgc catacacatc
    22681 aatcatttag aagcatcaaa catttatcaa tttcccgcac tattttatcc aggtgctgac
    22741 gggaatttct gcgttcttct tcagatccgc ccaatcccat tgtagtttgc aaaattttat
    22801 aatcattccg caaatccagg atttctttgt gagctgtcat caaatcttcc tgttttcttc
    22861 ccagttcggc ttttagagtt tcattctctt tgctcaaagc tttatattcc gaaatcagtt
    22921 ttttaattct tagctcaaat tcactgatta aacttgcgta tcgattttcc atttttcaga
    22981 ctaaaatctt cacaaaaata agattttgca ttgaaataaa aaactttttg gtcttttttt
    23041 atataatcag ttcaaaaatc aatcttttgc ttgctggtta tcaacctttt aaaattatat
    23101 taacttcgtt gcacgttcca aagcttcgtt gatattgcta cagatatttt cattacctat
    23161 ttttttgtca agctctacct gcagcaatac tttccggaca ttgtcattga cacccgagag
    23221 taaaacctgt gttttatctt ttttgcacat tttaatcaga ttttcaaggt tgtgtacacc
    23281 cgtagaatcg ataaaaggca cttttctcat cctgattatc ctcactttcg gtttatcgct
    23341 catctgtttc attatttctt caaacttatt ggcaagtcca aagaaaaatg gtccttcaat
    23401 ttcatacact tccactcctt tgggtattaa taatttatcg gtgtcaacag ctccttccac
    23461 ataatccgcg gcttccactt cactctctaa tactgaaatc gtggaggttt cggatatacg
    23521 gtggataaat agcacgacag ccagcaacag tccaacttca atagcgatag tcagatcgaa
    23581 aatcaccgta agaaagaaag ttgcgaggag aacgcttaca tctgattttg tatttcggag
    23641 taatgattta aaggtacgcc attcgctcat attataagca acaattacca acacaccggc
    23701 cagacaagcc attggaatgt gtttggtcag gttaccaagg aaaagcaaaa tcagtaacag
    23761 aacaaatgca tgtataatac ccgcgatcgg tgtacgtcct ccgttattga tatttgtcat
    23821 ggtacgggca atggctcccg ttgccggtat tccgccgaaa atcggggtaa ttacattggc
    23881 taccccctga gcaatcagtt ccgtattaga attgtgtcgg ctgccaatta caccatcggc
    23941 gactgtagct gaaagtaatg actcgatggc accaagcatg gcaatggtaa atgcaaccgg
    24001 cagtaaagta cgaacagtat cgaaatcaat actgaatttg cttgcctgag gtaacgcggc
    24061 gttgatagaa aaacggtcgc cgattgtttc gataccctgt acgccaaagt tgtgtttaag
    24121 tacataaaca acaatggtca tcaatataat cgctatcagt gaaccgggaa ttttttttga
    24181 gaatttaggt gtgattgcga ttattactat agataaaatc ccgataaggg ttgcccacaa
    24241 gttaatagta gtaatatttt gcgcgtaaac aacccattta tgaataaagt ctgccgggac
    24301 tttttcaatt tgcagtccaa acaaatcctt aatctgagtt gcaaaaattg tcagtgcaat
    24361 accgctcgta aatcccacaa caaccggata aggaataaat ttaatgattg ttccaagttt
    24421 catcactccc atcgcgatca gcatcaatcc cgcgatcatt gtagcaatag ccagccccgt
    24481 aattccatac tgctgtacaa ctccgtaaac aatgactata aatgctccgg taggtccacc
    24541 aatctgtacc ctgctacctc cgagcaatga aacaataaaa ccagcaataa tggcagtata
    24601 caatcccttc tcgggcgtca cacctgatgc tataccaaaa gcaattgcaa gcggaagtgc
    24661 aacaattccg acgatgatcc ccgccatcaa atcggaataa aattgtttct ttgaataatg
    24721 ctttaatgta taaacaattt ttggatcaaa gaatttcata tccttagtat gttaaaatga
    24781 tttaaaaatg aaaattgccc tgaaagtgaa cctgaaagaa ccttttactg ttttttatgg
    24841 ttttactctt ggacaattca tacacaaatc tgattacatc aaatcgaatg caaaggtaat
    24901 caattttatc aagaattatt tttttttatt cataaagggc aataaagttg taaatttgtc
    24961 atttcattta ataaaaaaca gaataaaaga tatgtgggaa tcagatccat acaattatca
    25021 tcgccgggta acggatgaag ttaaagctgg aactttgaaa attggaggtg aaaatccggt
    25081 cagagttcag tcaatggcca acacgaatac aaacgacata gaaagttcaa cagctcaatg
    25141 tcttaggata gtcgaagcag gtggagaact agtcaggttt accactcagg gactaaagga
    25201 agttgaaaat ctgaatatta ttcatgcgaa gatccgtgaa gctgattgtc atgttccgct
    25261 tgtagcggat gtgcatttca acgcgaatgt tgcagatatg gcagcccgtt acgtggaaaa
    25321 agtaagaata aatccgggga attacgttag cggaataaaa acaaccctaa catccgatta
    25381 taccgaggct gaataccaag aggaattcga aaaaattcgc accaggttta aagcgtttct
    25441 cgacatttgc aatacatacg gcacagcaat tcgtatcgga gtaaatcacg gttcgctgag
    25501 cgaacggatg ctaaaccgct ggggtaacac tcctgagggg atggtggaat cttgcatgga
    25561 attccttcga atttgccggg aagaaaactt caccgatgtt gtcatttcga tgaaggcctc
    25621 taacaccgta ctgatgattc agtcggtcag attactggta gaaaccatgg aaaaagaaaa
    25681 cctgcgcttc ccggttcacc ttggcgtaac tgaagccgga gacggagaag acggccggat
    25741 aaaatctgca gcaggtattg gaggattact ggccgagggt attggcgata ctatacgggt
    25801 ttcgctgagc gaagaaccgg aagccgaaat acccgtgaca aaactactga gagactatat
    25861 tacacagcgt gaaagccatg aggcaatacg cttaaaccca gaatataaaa tttcaccctt
    25921 acaattaagc aaccggaaat ctaacgcggt aaaaaatatt ggcggagaaa atgtaccggt
    25981 agttatttcg cgtccctgca accaccaaaa cctgaaggca gattatattc ttctggatga
    26041 agaaatacaa aatacaagtg gtgagaaatt cggtgtttat gcagataaag ctccggaggt
    26101 aaaagtttct gaactttcat ttctggaatt aaactactct cggctagact ataaaaccct
    26161 tgaatactta aaaacgaatc cgcagatagt catactactc agtaccgacc acattaatcc
    26221 gcccggagaa caaagagcat ttattcacac gctgatatac gaaggctgca ataatcccgt
    26281 tattgtggtt ggcaattacc gggaaaatga tttggagtct cttcaaataa aagccgcggc
    26341 tgatctaggc attctctttg tggatggact ggcagacgga attctgataa agaacgaagg
    26401 cagaattccg gaaaatgaaa ttctttcaat ttcgtttggt attcttcagg ctgcacgtgt
    26461 acgggtaagc aaaaccgaat tcatttcttg ccccggttgt ggcagaacca tgttcaacct
    26521 ccagaccgta gtagcagaaa tcaaaacaaa aacatcgcat ctgaccggat taaaaatagg
    26581 gatcatggga tgtattgtta atggtatcgg cgaaatggct gatgccgatt acggatatgt
    26641 tggagccgga cgcggaaagg tcagcctgta taagaaacaa acctgcatcg agaaaaatgt
    26701 acccgagagt gaagctgttg agcacctgat aaatctgata aaatcatacg gagactggaa
    26761 agagccggaa ttgtaaagct gctttctaaa catattcaga aaataaacgt ttaatatctt
    26821 aaaatcaaaa gaataatttt aacacaaaaa aaaacactca ttacccatga agaaattatt
    26881 tatagcaatc atcctgctga cagtacttac aaatttgaat attgctcagg caaccgacaa
    26941 agccatcggt attcgtggat taagtaattc cgctgaaatt tcttttcaaa attcgctgag
    27001 caaatccacc cgacttgaac ttgatttggg gctttacgga cttgacgatc gaaatggttt
    27061 cattctaaca ggtattcatc agtgggtttt cggacttgac aaaggtctaa actggtatgt
    27121 aggcctcgga ggtcagttag gttcacgctg gtacgagaaa aacaacctgt ggcaatcggg
    27181 tttcggactg gcattagcag gtcagattgg tattgaatac aattttacca ttcccgtaca
    27241 ggtttcactg gattatcgtc cggcgtggta tttaactcac ggaggcactt acgacaccgt
    27301 agctcttggg attcgttaca gattttagtc tttatttcaa aaatcagctt tcggatttac
    27361 gaatatgttc ccgtaaacag attctgcaag ataacaaatc atcattttta actctaaaaa
    27421 aatcaacatc atgaaaaaac tttttctggc cattgcactt ctgattgctt catcaagtat
    27481 cattcaggcc caaaccatgg acaaagccat cggtattcgt attacagatg gcgctgagtt
    27541 atcatttcaa aatccgctcg gaagttctac ccgcgctgag gtagatcttg gcatttatgg
    27601 taataattct gtcatcctca caggcatcca ccaatgggta ttcggacttc aggaaggtgt
    27661 gaactggtac gcaggtctgg gacctcagat aggattatgg gataaacttt atagcagcgg
    27721 aaaacactta gcactgggcg ttgccggtca ggtggggatt gaatacaatt tctcagtacc
    27781 tgtacaagtg tcgctggatt atcgtccggg atttaccatt tttccttcgg gacatgatta
    27841 caactcgttt tgggcgattg cactgggaat cagataccgt ttttaagtat gctgattact
    27901 tttataaaca cagaaaccct gaaaagaatt gattttcagg gtttctttta tattgttaag
    27961 tttattttgc tgaaccggta agattaatta ctttatcgtt tagtccgcca ccggtaatgg
    28021 tgagaacagc agtatgcgtt ccggcagtaa gcggtttata agttaccagc aaatcaaatc
    28081 cggaagtcga gttggctgtt gtctgaggta tgctggcagt acttattgta aaataatcag
    28141 cattggcacc agataaagta actgtgagat ttccgttaat gcctgtagtt actatattca
    28201 gattcttggg attagtttta ccgactacgg taagcggata agtaagttga ccgctaatca
    28261 tacccagttt tattttgggt tgcaagccac ccatcagttt aaaggtgaca ttttcggcag
    28321 ttttggcaat ttcggttatg tttctgtcaa tagatgtacc gtcccaggct gtcggtgtaa
    28381 agctacctga tgtaaatgca tctcccggag tggttgtaga accggacaaa ggctgcaaat
    28441 ataccctcat gtggcttgcc tgagtttgtg tgctttcgtt gtaattattt ggtccattat
    28501 tttcccaggc tgtctgatcg taatcgatgt gccagatgag catcccctct ccgggcagat
    28561 aggcatccca gccagttttt ttgcggtatt caaccataaa gaattcagcc ggttttgggt
    28621 cagccccatt caggtcatga gtaattgctg agagaagata agcctgatta tctgtatttg
    28681 caggtatggt tgttccctga tacaagggta gcagtgtcac caagttcgcg ggctgattaa
    28741 gttctacggg tttgtaatat cccagataaa accgatcgta cgcagaatac acaggtggag
    28801 tatttccctg gttcagataa gctccttgat ccataatact ccagttattt agagtattat
    28861 cctgattcaa agacgtgtcg tagtaatcgg gcaaaccaag tacatgtccg aattcatgac
    28921 agaatgtacc gataccacac attttcgaat cgctgtcgcc tttcagttcc gatgtacagg
    28981 cataatccat gatttttttc ccgtcgaagg taatggaagc tacggtaccg gcataatttc
    29041 cctcatctcc atatgttacc gtaggataaa ttccccagcg atgcggccaa actgtatcat
    29101 cgccccctaa ctcagcctcg ttgtaaccgg cataatatac gaatatatta tccacgtatc
    29161 cgtcgttatc ggtatcgtat tgcgcgaaat ccactccgtt attatcggct gccgtacatg
    29221 catccacaat catctgaaca ggatttatgt cataaccatc ctcatcattg ccaccataaa
    29281 ccgacatgtt tgatgggaga tcaaacggtc ctaccacatc gaactgtgga gaaaatttac
    29341 cgtacgagca tgccataaaa taatcttttg cagaacctgt accgttgttt tcactatatc
    29401 cgggctgatt aagcaggttg gtgaacgccg tctggatgga tggtaccaca aacgatttat
    29461 ctttgaaatt gaccagaata atcagcgatt taccggaccc ggtaaccggg aaagctcttt
    29521 tcattacagc tgcattctgt ttagttgatt ttactttcct gataccggat tgaaatttcg
    29581 ttgtcagttt ctcaggcata cgcaccagat atttgttgtc ggcagcctga cgtaccgaag
    29641 gatctctggc aatctgcgta cctgcaatga tttttccccg tgaatcgata cttgcatagg
    29701 tataaaaacc gctgtcaatc ttcttaacaa tataattgtc ttcggtagtg gtgtaatgat
    29761 gatattcgtc gccatgcatt cgaatattca atgaagatcc atcgggctgt gtgattttga
    29821 cgattcccgg ataggctctg attgcaaaag cgctcagtag tggcgcacat aatagtaaaa
    29881 caactaaagc cgatttaata aagatgagtt ttttcatttt taatattttg acataaaagt
    29941 cgcaaatata tgatttcagt ttggattaca aaatacacaa ctcaaaattt tccaataaaa
    30001 aaaaaagctg tcctttatag acaactcttt taattattca ttttttggta catcaggatt
    30061 ttcagaggct tcgtctttat taaatttttc tttcacatta ttaaatgttt ccttcatttg
    30121 tttcaggatt aactctgcac cgtctttcac ttcgtcccat gcttcgtctg ctgtacctgc
    30181 caattcagcc agcctgactt taccttcaac tattttaggc tccagctccc tgattttttc
    30241 ctgatattca gcctgcaaat cagctgctgc gttctgtact tttatcttca ggtcttcaag
    30301 atcagttttc aagtcttcga actgtgcatc tactttttgt cgataagatg atttttcttc
    30361 cataaatatt ttttttttga taagtattat ttagcaaaaa taatcatctg atcataaaac
    30421 cggaataata acatgttaaa atacacgaaa accggtcaga tttacagcca tttttttctc
    30481 cggaagtacc acaataccgc tcctgagaac gccaccatca ggaataggga gaacgggtaa
    30541 ccataagccc aattcagttc tggcaaaacc ttgaagttca tgccataaat actggctata
    30601 agcgtgggag gcaggaatat gacagacacc acggtaaata tcttgatgat tttattttgc
    30661 tgtaaattaa ccaaaccgag gaaagtatcc tggagatagt ccagacgatc gaagccgaaa
    30721 cgcgtatatt cgaacaacga gttgatgtcc ttgataatca tgctcaggcg cggcagtaat
    30781 tctgcgggga acacatcgct tttaagcatg ctggatatga cgcgttgctt atctattatt
    30841 gtctgacgaa tgatcatcac cttttcctgc aggtccttga tacggatgag gatatcttcg
    30901 tccagatcat cttccttatt catggtttta ctgaggttgg taatgaggtc ggtggtatcc
    30961 tcgataacat cggcatcgta ttccactcgg gtttccagca atgcaaccag tacgtgatat
    31021 cctgtcggga agcttcgggt actggcaaag attttcttta cggtttcgtt gaacgagcgc
    31081 agatcagcat cacgtaccga tacgagtatt ccattcttga ggataaacga aacgggttcg
    31141 agtgtgaaat tatccatcat gaaattggta ttggcaacaa taccgtcatc ggtttcgata
    31201 tagcgcgaac tcatctcgat ttcttcaatt tcttcgtctt cctgaatgta tattttcaga
    31261 aactgctcca ggcggctttc cacatcgagt gctacatcat tgaggtcgat ccatataata
    31321 ttcttcagat caattttcgg gagtatatcg aggcttttcg atatttttat tttaccatct
    31381 tcgtgataaa aaagtcttaa ttctgacatg atttttctct cctttctttt ttatcaaaac
    31441 aggattccgt aattctgatt tgatattcat tttggaaact ggaatttata agacctttgc
    31501 tgttttcaca aagctgggag aaaccggtta ttggttctct ttcagagctt tttccaccat
    31561 ttgtgtaagc tgaacgaatt cagccacgcc cagttgttcg ggacgtttgt cgaaaatggg
    31621 atcgacatac atggaacttt ctttgggcac gagaggtttc agtgaattgc gcatggtctt
    31681 tcggcgctgg ttaaaggctg ttttcactac ggtccggaag agtttctcgt cgcaatcgag
    31741 atgttgcact tcgttacgtg taaagcgaat aacagccgat ttgaccttcg gcggagggtc
    31801 aaagacctgc tctgatacgg tgaagagata atcggttgta taaaatgcct gcatcagcac
    31861 actcaggatg ccataagctt ttttgcccgg agccgaagca atgcgctccg ccacttcctt
    31921 ctgaatcatt ccggacacat aggggatacg atcgcggtag tcgagtacct taaagaatat
    31981 ctggctcgaa atgttgtatg ggaaattgcc gataatacag aatttatcgg gataaagcgc
    32041 tgaaagatcc attttcagga aatcgccttc gatgaccttc agctgcggat aatgcaggtg
    32101 aagataatcg accgactcgc ggtcaatttc gacggcagtt aaatcaacga gtggattttg
    32161 gagcagatac tgggtcagga cgcccattcc gggtccgact tcaaggacgg gcattgcctg
    32221 gctgagaggt aaactctctg ctatac
//
LOCUS       DAOPYB010000017        31279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1386_length_31279_cov_9.716820, whole genome shotgun sequence.
ACCESSION   DAOPYB010000017 DAOPYB010000000
VERSION     DAOPYB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 31279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31279
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1386_length_31279_cov_9.716820"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..126)
                     /locus_tag="PKH58_02605"
     tRNA            complement(53..126)
                     /locus_tag="PKH58_02605"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Ala,seq:ggc)
     gene            203..511
                     /locus_tag="PKH58_02610"
     CDS             203..511
                     /locus_tag="PKH58_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNX87949.1"
                     /translation="MFIYNTTFLVTDRRFNPWFKWLKEEHIPNMLNSGYFLKPQIAKV
                     LTAESDQEGMSVSVQFIVNDLENLQNWIDNNEIRLQNDLSLRFGAEVLSFSTILELMD
                     "
     gene            514..1074
                     /gene="ruvC"
                     /locus_tag="PKH58_02615"
     CDS             514..1074
                     /gene="ruvC"
                     /locus_tag="PKH58_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNX87950.1"
                     /translation="MAKDQIILGIDPGTTVMGYGIIRVKDNKAEMITMGVLELKKYSD
                     HYQKLQRIFARTLSLIDEYHPVCLAIEAPFFGKNVQSMLKLGRAQGAAIAAALYRDLP
                     VNEYAPLKIKMSITGNGRAAKEQVADMLRRYLRIPQENMLPQLDATDGLAAAYCHFLQ
                     SGKPTVDSEYKSWKDYISQNSSRVKK"
     gene            complement(1120..2217)
                     /locus_tag="PKH58_02620"
     CDS             complement(1120..2217)
                     /locus_tag="PKH58_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNX87951.1"
                     /translation="MKKTAFLISLVVLIFSAALHGQKNPSERKYKLVAYYSTDSASLM
                     MYDYSRITHVIYSFGHVNSDGYFGVTRQKDLTVLKELIKLKQKYPHLKTMIALGGWGG
                     CELCSNVFNDPQKTKRFVSSVRDFLKTYNLDGFDFDWEYPVVKGFPGHQYLMDDKNNF
                     TKAVALLRKTLPKRKIISFASGGFIEYMEKAIDWKAINPYIDFVNLMSYDLVGGYATT
                     TGHMTPLFSTWENEESADKAISYLEKIGFPLHKVIIGATFYTRSWENVPNINNGLYQP
                     GKFLGSHDYKMNVNIFTESNGYKYYWDEKAQAAHWYNDEKMIFVTGDDKQSLKAKSDY
                     VKKKGLGGLMFWELPLDMPENGLLDVIDLNR"
     gene            complement(2379..>3416)
                     /locus_tag="PKH58_02625"
     CDS             complement(2379..>3416)
                     /locus_tag="PKH58_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNX87952.1"
                     /translation="ESSFNSDTLPFIQNIIKQTEALQQVASRFQSQLIQINSVKPLDE
                     ELLQNRLQAANGYFSEHLSILTDTLRQTSASTDNRQNANEYDDFLEDIYNFIEQKKHL
                     IKGIQNGFNPEKYFELRHNFVLPLFSHNSYSKTNSNQKISSRHPQLLYQLFHIRNEIS
                     DRTGLPIYLIATVKSLTEMADYLPQTERDLLKISGFGKPKVDKYGEDFLNCICTYCIE
                     NNLSSEMSLLEAKGTKKTAQKKEKKPKIDTVRVSLELYLEDNTIPEIAKQRNLTEGTI
                     TKHLSELVEKGVLKVERFISPEKLQRAVSLLEKNNLNGSVYELLSSVLSQQEVTFMLS
                     WIRGGKKEGKA"
     assembly_gap    3417..3516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3517..4604)
                     /locus_tag="PKH58_02630"
     CDS             complement(<3517..4604)
                     /locus_tag="PKH58_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX87953.1"
                     /translation="MNITSELHDFAREFAELTNCNIFLTGKAGTGKTTFLHELRKNTG
                     KQIAVVAPTGVAAINAGGTTIHSFFQLPFTPFIPTPEGKKDLISKLKMQSHRRKVIRE
                     VELMVIDEISMVRADLLDAVDTLLRHIRYRNNEPFGGVQMIFIGDMYQLSPVAQSEEW
                     KLLSPYYESIYFFHSRVIQEKPPVYIEFEKIFRQRDMSFISLLSEVRNNNLSEKGMSM
                     LQSRYNPLFVPPKDDTYIILTTHNYKADRINSEELAALKNKSRYFEAQVKGDFPEKLY
                     PIEEKLELKVGAKVMFIKNDTETPKRFFNGKIGKITAFEDETIIVKCPDDDQEIEVSP
                     MVWHNVRYKANETTLQIDEEVLGTFTQFP"
     gene            complement(4815..5264)
                     /locus_tag="PKH58_02635"
     CDS             complement(4815..5264)
                     /locus_tag="PKH58_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNX87954.1"
                     /translation="MRKYKFTINEKEYNVVVKGIDDEIAELEVNGNPYEVKIHQLEQK
                     VPKTPILVRKEVQNKPGENKVAEKLAPMPITAKPSSKTINAPLPGSVVKINVEAGSSF
                     NEGDVLMIMESMKMENNILAEKSGTIVKVCVAPGASVMQDEALFEIN"
     gene            complement(5301..5618)
                     /locus_tag="PKH58_02640"
     CDS             complement(5301..5618)
                     /locus_tag="PKH58_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87955.1"
                     /translation="MLVAILAVLAVALVVVVFLLIKKNKDQKEALAEKQVYKAQPAVD
                     NVVKAESAPGNDEEVAAFLALHLYLTQNVHDLESNVITIERIQKRYSPWSSKIYSINN
                     FQR"
     gene            complement(5655..7214)
                     /locus_tag="PKH58_02645"
     CDS             complement(5655..7214)
                     /locus_tag="PKH58_02645"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNX87956.1"
                     /translation="MKNQEKVQDLIDKRVQAKLGGGEKRIDSQHKKGKLTARERIDML
                     LDDGSFEELDMFVTHRCTNFGLEKEKYMGDGVVTGHGTIDGRIVFVFAQDFTVFGGSL
                     SETMAQKICKVMDMAMKMGAPVIGINDSGGARIQEGVTSLAGYAEIFQRNILASGVVP
                     QISAIYGPCAGGAVYSPALTDFILMTEDNSYMFVTGPKVVKSVTGEDISTEDLGGADV
                     HARKSGVTHFKLVDEKEGAMLIRKLISYMPQNNLEEPPVYPTNDPIDRLEDSLNSIIP
                     DNPNHPYDMKDVIHSIVDNGEFLEVHRHYARNIIVGFARFNGASTGIVANQPNFLAGV
                     LDVDASRKAARFVRFCDAFNIPVLTLVDVPGFLPGSGQEYAGIITNGAKLLFAYGEAT
                     IPKVTVTIRKSYGGAHDVMSCKQLRGDFNYAWPTAEIAVMGAAGAIEVLEGRTIAGIT
                     DPDEKAKYVTEKELEYKEKFANPYEAAAYGYIDDIIEPRNTRFRVIRAFEALQTKKVL
                     NPPRKHSNMPL"
     gene            complement(7265..7696)
                     /gene="mce"
                     /locus_tag="PKH58_02650"
     CDS             complement(7265..7696)
                     /gene="mce"
                     /locus_tag="PKH58_02650"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNX87957.1"
                     /translation="MKVTHIEHLGIAVNSIEEALPFYENILGLKCYAVEEVADQKVKT
                     AFLQVGQTKIELLESTDPEGAVGKFIEKRGEGIHHLAFAVEGLANALKEAEEKGIRLI
                     DANPRKGAEGLNIAFLHPKSTIGVLTELCENPNKAPGNSCC"
     gene            complement(8036..8458)
                     /locus_tag="PKH58_02655"
     CDS             complement(8036..8458)
                     /locus_tag="PKH58_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNX87958.1"
                     /translation="MTINETQNEIIEEFNQFDDWMDKYALLIDLGNTLQPLDDKYKIP
                     QNIIEGCQSRVWLNAELIDGKVVYQGESDAVLVKGIVTMLIQVLSDHTPEEILDTDLY
                     FIEKIGLKEHLSPTRSNGLLAMVKQMKLYALAFKAKMG"
     gene            complement(8633..9856)
                     /locus_tag="PKH58_02660"
     CDS             complement(8633..9856)
                     /locus_tag="PKH58_02660"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNX87959.1"
                     /translation="MTEQEINNIRADFPILMERVYNKPLIYFDNAATTQKPQAVLDRI
                     NFGYKHLNANIHRGVHYLSQKATEAHEEARRQIARFINTASSDEVIFTRGTTEAINLV
                     AFSFGEYCCRAGDEIIISMMEHHSNVVPWQMLCERKGMKLRVIPINEKGELRLDIFKD
                     LINENTRLVAVTQVSNVLGTVNPVKEIIQTAHLHNIPVLIDGAQAVPHIQVDVQELDA
                     DFYVFSGHKIYAPTGIGVLYGKREWLERIPPYQGGGEMIQTVTFEKTTYNELPFKFEA
                     GTPDYIGSTALAEALKYVENIGIKKIAAYENELTAYATAELMKIDGMRIFGTSEHKSA
                     VISFLVGNIHPYDIGMLLDKLGIAVRTGHHCAQPLIDYLGIPGTVRASFSFYNTKEEV
                     EIFINALKRVVGMLG"
     gene            complement(9989..10921)
                     /locus_tag="PKH58_02665"
     CDS             complement(9989..10921)
                     /locus_tag="PKH58_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX87960.1"
                     /translation="MKKMHFINGDRFPQIGLGTWLSKSSEVYNAVIEAIGAGYRHIDC
                     AYIYKNEKEIGEALQYAFSTGLVSREEMFITSKLWNNFHAPQDVEKAIGISLENLKLD
                     YLDLYLIHWPLAFRQEQALSVGDLVSLEEIPLTSTWKGMEAVHKSGLTKHIGVSNFNI
                     PKLKNLIANSEIKPEVNQIELHPYLQQNELVRFCQDNNILVTAYSPLGSHHLTDNENS
                     ILLHPVVKEIALKNHCTEAQVALAWGIQRSTVVIPKSVNPTRIQENRGAEYIQLDEKD
                     MEQIMKLDRNHRNAKALFAVFPDGPYTLRSIWEE"
     gene            11134..13035
                     /locus_tag="PKH58_02670"
     CDS             11134..13035
                     /locus_tag="PKH58_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNX87961.1"
                     /translation="MKKLFLSLTLFLFGSSVFNQTFAGKFNIIPEPVAQVEKPGNFTL
                     PKNVNILRPAGNENDITVNFLKEKLSKATGSKVTVRSSGKSDIQLILNKVNDTMIGEE
                     GYILSVRSKKITIRANKPSGLFYGAQTLIQLFPAQIESEEVVNNVEWKIPAVEIGDYP
                     RVAWRGLMLDVSRHFFTVDEVKQYIDAMVRYKYNMLHWHLTDDEGWRIEIKSLPKLTE
                     IGAWRVEKIGNFGEFSAPLPDEPKTYGGFYTQEQIREIVRYAADRFVNIIPEIDVPGH
                     SLAAIASYPELSCTEGADKYNVRAGEQFMDWSHGAPPIAFIDNTLCPANEKVYDFMDK
                     VMTEVAQLFPYPYIHVGGDEAPHNFWQKSAQVQELMKREGLTTFPQVQSYFEKRLETI
                     VLAKGKKMMGWDEIMEGGITPTTALMSWRGVKYGIEASRSGHYVVMSPTTFAYIDYMQ
                     GDVCTEPPVYSSLRLNKTYQFDPVPPEADARYILGGQANLWTEQVYNIRQAEYMTWPR
                     GFAIAESLWSPKDNKNWENFVSKTENHFHRLDYAETKYSPAIYDPIVNVMKKDDKYYV
                     ELTTEIGGLDIYTSFDNSTPDCFYQRYTEPLSLPKDARLMKIITYRGKVPVGRLMTIP
                     IDDLKKRAK"
     gene            complement(13079..14233)
                     /locus_tag="PKH58_02675"
     CDS             complement(13079..14233)
                     /locus_tag="PKH58_02675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX87962.1"
                     /translation="MTNYKNISFFNGLNSLRFFAALLVVLHHSETIRFKNGLPNLNWL
                     GLFKDGSIAVTFFFVLSGFLITYLLLKENGQNGTVSIKRFYLKRVLRIWPLYFLLVIF
                     GTLLLPALINIFGLNYVMPYTFGQTWHYFLFFLPGLVTYFFGHHLLEPLWSIGIEEVF
                     YLIWAPLFKFAKKYLLLILISIIAIKSFLIITSFYIVENNLFSYLVDIFQFEAMAVGG
                     LGAYFIYNRNHSLSGLKIYKIPVQIAIYSLLVIFLVFNRNIDNSVWNIFFKTPVFSQL
                     FICFLFLYLIIGVSLIDHSIFKLRKRFFSYLGEISYGIYMYHMPIIFGLMLVLQKQLS
                     KLDLLTGSLLYYPVTLIVVVGVSAISKRYFEDFFLKLKDRIVIRKKVTIQ"
     gene            14496..17009
                     /locus_tag="PKH58_02680"
     CDS             14496..17009
                     /locus_tag="PKH58_02680"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNX87963.1"
                     /translation="MNKTILNSLTPHLVALLLFIVISFSYFTSVLEGKKLIAHDTQSW
                     IADSKETVDYNESHDDVTLWTNSLFGGMPNYQISMEQPNNVLQYVEKLVMEFPRPVSN
                     LLLYLICFYILLLAFGFKPWMAIIGSIGFTFASYNFIIIAAGHNSKAITIAYMAPLIG
                     AVFLAFRRKRLLGGILTAFFLSLAIRANHIQIIYYTLIVLLIFAVVEFIYSIREKEIK
                     EFLKTAVVMIAAALLAVGMNATSLLTTYEYSQYTMRGKSNGLTTAVQNSPNAVNTGHG
                     LDKDYITQWSYGVDETMTLLIPNFKGGASGGKLSMNSETAKYLKNMNVPDSQTQQIVS
                     QMPLYWGTQPGTSGPVYLGAIIIFLFVLSLFVVDKRTLWWLIPVIILTLMLSWGRNFM
                     WLTNIFIDYFPMYNKFRTVSMTLVATGFGITLLAFLALKEIFDENVNKEKLTKSVVLS
                     MSITAGICLIFALLPSLAGGFVSPQDAQFSGDYSFLKETLPLDRKAMLRADAWRSLAF
                     ILLGAVVILAYIKGFLKKNVALVLLGFFILIDLFPVAKRYLNDGNFENKRPAQLIQPT
                     QADQIVLQDKSQYRVLDATVDIFSDATPSYFHKNIGGYHAAKLRRYQELIENQIQPEI
                     GQLFGAFGKATTAESILPTLESLGVLNMLNLKYIIYNKESAPLVNPYNNGNAWFVQKI
                     RIAANADDEIKLVGEIDTRHELVVDKSLASIFPTTLTPDTTATIELASYAPNHLVYKF
                     NSTTDQVAVFSEIYYDKGWKATINGKEVPYTRVNYLLRAMPLKAGNYEIDFRFDPQSY
                     KAGNMLAIVSSALLLIAIALLAFFYQRKRKTTTPKPGKN"
     gene            17075..17956
                     /locus_tag="PKH58_02685"
     CDS             17075..17956
                     /locus_tag="PKH58_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNX87964.1"
                     /translation="MSTKKYKKRTFRFRNANLTATFSMSLVLFLVGLLTLTLFLARDM
                     TRYVKENLNLSIVLSDEITPRQTERIKKFLEKSDYAKSVELISKEQALQEHIQDLGEN
                     PEEFLGFNPLLASLEVKLNENYANTDSVAKIEKKLSKYKEIQKVSYQKDMISMVNENI
                     KKISIILLGIAAILMLISFALINNTVRLSIYSNRFLINTMKLVGATHWFIRKPYVRRS
                     VLNGIIAAIIALGLLTGLVFYFQNEFGLQSQMITRDTELIVAAVVIVLGILLTAISSY
                     FAVGRYLRMRSDDMYYI"
     gene            18054..18281
                     /locus_tag="PKH58_02690"
     CDS             18054..18281
                     /locus_tag="PKH58_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNX87965.1"
                     /translation="MENKKFGLSKINFILIAVALLVIIIGFLLMTGKPSGVEFNPDIF
                     GTRRIIVGPMISFIGFISMIAGILYTPKNKN"
     gene            18284..19090
                     /locus_tag="PKH58_02695"
     CDS             18284..19090
                     /locus_tag="PKH58_02695"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNX87966.1"
                     /translation="MNVIQAIVLAIVEGLTEFLPVSSTGHMALVSALMGIEQESFTKL
                     FIETIQFGTILSVLVLYWKKFIDFKGFQFYIKLIIAFLPAAVFGVIFKKHIDALIGTP
                     LFIACTLFFGGIILLFVDNWFKNPTIDDEKQATNKNALIIGLFQVLAVVLPGLSRSAA
                     TIIGGMQQKMTRKAAAEFSFFLAVPTLAGAFVKSLWDAFQETPEILTQHNISLLVLGN
                     VVGFIVAVIAIKAFISFLARHGFRLFGYYRILVGITIILLILFGVNLSVI"
     gene            19112..19801
                     /gene="truB"
                     /locus_tag="PKH58_02700"
     CDS             19112..19801
                     /gene="truB"
                     /locus_tag="PKH58_02700"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNX87967.1"
                     /translation="MDFIEGEVLYVKKPLHWTSFDVVNKVRWKISRKLNKKKIKVGHA
                     GTLDPLATGVMILCTGKATKQIEDFQYQTKEYAATLELGATTPSFDREHEVDARYPTD
                     HITRGLIDEVISRFKGEIWQVPPVYSAVKVDGKRAYNYARDGQEVELKPKLLVIDEIE
                     VLSYDFPVLVIRVVCSKGTYIRALARDIGEALQSGAHLIALERTRIGDITLTDCWEID
                     DLLKQIDTLDC"
     assembly_gap    19992..20091
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20134..21183
                     /gene="queA"
                     /locus_tag="PKH58_02705"
     CDS             20134..21183
                     /gene="queA"
                     /locus_tag="PKH58_02705"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNX87968.1"
                     /translation="MKLSQFKFKLPDELIAQYPAVHRDESRLMVLHRKSGEIEHKVFK
                     DVLDYFNENDLFIFNDTKVFPARLYGNKEKTGAQIEVFLLRELNREGRLWDVLVEPAR
                     KIRIGNKLYFGEDDSIVAEVIDNTTSRGRTLRFLFDGTHEEFKRELYKLGQTPLPHNI
                     TRPVEPDDEERFQTIFAANEGAVSAPAAGMHFSRELMKRMEIKGINTAFVTSHMSLGN
                     FREIDVEDLTKHKMDSEQMQITPETADIVNAAHDAGENICAVGVTVMRALETVVGTEG
                     RIKPLDGWTNKFIFPPYDFTVANSLITNLHLPYSTLLMLTAAFGDYDHVMNAYDVAIK
                     EKYRFGVYGDAMLVI"
     gene            complement(21239..23038)
                     /locus_tag="PKH58_02710"
     CDS             complement(21239..23038)
                     /locus_tag="PKH58_02710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNX87969.1"
                     /translation="MDSFVVSARKYRPSTFKSVVGQDALTTTLRNAIKSKKLAHAYLF
                     CGPRGVGKTTCARIFAKTINCEHLTEEFEACNQCESCKTFNEQRSYNIHELDAASNNG
                     VDDIRSLTDQVRIPPQIGNYSVYIIDEVHMLSSGAFNAFLKTLEEPPAHAIFILATTE
                     KHKIIPTILSRCQIYDFNRISINDTINHLKYVASSEHVTIEESALNIIAQKSDGGMRD
                     ALSIFDQLVSFSGNAITYQNVIDNLNVLDYEYYFKLVNAFLQGNVSEALLIFDEVLNK
                     GFEAQHFITGLSSHLRDVLVSKDASTIQLLEVGTDIGERYRQQAQACPVEFIFDALKI
                     SNDCDLNYRISKNKRLLVEIALIRISQLMSDKKKNLTVDKPPLKPIEVSEPAIITQPV
                     ITSKPQVIVTQAPPTQPQPVVQTTPADTIVSEPDTTDVYAGNPVPKKTAEIKTVVSQA
                     PPRISISNIQSEKSTETASEGIKKHTDDNISDKEFTSQELIEKWKIFANQFQKDQVTR
                     GFLLNHLPSLENDNIIGLLLDNKFQEKEISQIHVLEYLRKELGNNSIRFQIDFIPEEE
                     QKTYLSPQEVYKKMVGANPALAVLRKNLDMEID"
     gene            complement(23245..23676)
                     /locus_tag="PKH58_02715"
     CDS             complement(23245..23676)
                     /locus_tag="PKH58_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87970.1"
                     /translation="MKANKYIIIFIAIFSVLLVFPAHGETVPPEWAPANGYRAKTRHI
                     YFPEYNTYFDLKKDVYIYEKDGVWFIVKSVPPKLKLENLKTSPQVQLYLDTDLPFTEN
                     EAHKSKYNYLARHDENSQLEINKSLKRQQREIKKIKKGLNK"
     gene            complement(23810..24754)
                     /locus_tag="PKH58_02720"
     CDS             complement(23810..24754)
                     /locus_tag="PKH58_02720"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNX87971.1"
                     /translation="MSEDSSLFKVFTGTNSRYLAEKICKSLNWPLGKMNIEHFSDGEF
                     SVSYEESIRGQYIYLVQSTFPNSDNLMELLLMIDAAKRASAYKVIAVIPYFGWARQDR
                     KDKPRVSIGAKLIADMLTIAGVDRVITMDLHADQIQGFFNVPVDHLYGSTIFIPYIKS
                     LQLENLVIASPDVGGSKRAGSYSKHLDVPMVLCHKTREKANIVGEMRIIGEVAGKNVI
                     IVDDMVDTAGTLCKAADLMLENGALSVRAIVSHGVMSGEASKRIMNSALTEIAFTDSI
                     PFDCNKCSKAKILTIADMFAETIHRVQEHKSISEQYLL"
     gene            25123..27813
                     /locus_tag="PKH58_02725"
     CDS             25123..27813
                     /locus_tag="PKH58_02725"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNX87972.1"
                     /translation="MRIHFKIPYFTKWGQRILVSGDVPELGDGDYLKALPLRFQGHED
                     WVNDVECRVAEKQVVHYKYLIYDENTNSFTEEWGNDRVIDQLTLKTEHYFCYDTWNSI
                     GSHENVFMTSPFQEVLLKGNTTTSPNGKNKKFTHIFKVKAPLLHHRQTLCIIGSCGEL
                     GEWSVAEPLLMVKDGDNWTIKIDLSKRTTDVNYKYGVYDADEKRFMFFEQGPDRYAAV
                     IGSKNTIVQVHDGFVRMNNDSWRGAGVSLPVFSIRTRRSFGVGDFEDMKMFVDWAAKV
                     NMKLIQVLPLNDTNGTHTDADVLPYAAISAFALNPLFLNLPAMGKLPENNAIGQQYIP
                     KQAELNAYPLISFLEVIDFKMKYAREMYLYQKTEFLNNPDFIQFFEKNSYWLVPYAAY
                     CVLRDKFGTNDYRNWEEFAVYDAGQISAFTDKTQPHFDEVGVNYFIQYHLHKQLSEVA
                     QYAHQRGVVLKGDIPIGINKNSVDTWVSPELFHMDMNAGAPPDMFAVKGQNWELPTYN
                     WEMMKRTGFGWWKKRFEQMSNYFDTFRIDHILGFFRIWEIPTNQVEGIMGHLNPSTPI
                     YLNEFGEKGIWFDYNRFCKPYITDQILWDFFGEEAAWVKTNCLKIEDGWVLRLKPEFQ
                     SQTYVEELFNQGEISERVKWGLFDLIANVLFFEVKDSNGQQFYPRYGMHSLRSYIELD
                     QHTKKGLDELYIHYFYHRQDENWYHSGMEKLPALKRSTNMLICGEDLGMMTPCVTAVM
                     NELGILSLEVQRAPKTSKIDFFHPADAPYLSVVTPSTHDMSTIRGWWEEDRAVTQKFY
                     NTQLGHWGDAPFFCEWWISRDIILQHLYSPAMWAIFQMQDLLGMSPKLRRENPHEERI
                     NVPSNSMSSWRYRLHLNMEDLLQEDEFNNELRNFIVQSGR"
     gene            complement(28101..28478)
                     /locus_tag="PKH58_02730"
     CDS             complement(28101..28478)
                     /locus_tag="PKH58_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87973.1"
                     /translation="MKDYKETNLEYTLTNMYKDDLISYIASHQDDFPELIKLSISDKK
                     PYSWRAAWLLWSCMNNNDKRIRRHIKMIIDILPNRQDSQQRELLMVLQRMELDSKYEG
                     QLFDSCTKIWENINKNSVERRLA"
     gene            complement(28663..30981)
                     /locus_tag="PKH58_02735"
     CDS             complement(28663..30981)
                     /locus_tag="PKH58_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNX87974.1"
                     /translation="MKNLFTIMLFALLANILTAQVTKTIDVTTPGTLTTLLTADEKTT
                     VTDLTLTGNIDVRDVKCMRDEMTILANIDMSAVNIMEYFGSDGTFSYTTYPANEIPVH
                     SFSTFGKTIGKTSLKSIQLPTTLTSIRTNSFYKCKGLTSIVIPNMVTSIESQAFYNCS
                     GLTEVTLGSSVSTIEDAVFYQCSSLNTITSLNPTPPTLGTNTFDFDSSSNSRVVILVS
                     ASSVNAYKAATGWNAFNIGSTEKITIHVPTSGGLAAALIAAGHIPLSGIASLTITGNL
                     NSTDIIQLKNSLTSLIDLDLSGAMLAGNSLPASAFQEKKLLRLVKLPFSLETIGDYAF
                     YGCSNLTSVLPLPAGLISIGHFAFSECRSLSGDLIIPNSVTVIGDKAFRSCSGFNGEL
                     RLPNSLKSIGDGAFYICSGLTGKLTLPNTLTTIPDQAFYDCSGLTGEVIFPQSITQIG
                     YYAFEACSKLTELTFGKNVVNISNGAFQGCSSIVKITMPRTTPPLITSSTFTGVNIET
                     CTVHVPIGTAATYQSDPIWGLFILFNETGAAETYSITVYLGANGTIKENNVALSNGSV
                     LTVTPGSTKIFTLVPDAGFEVATMTYNGVDVRSQMNNNVFTTPAINANATLSVTFQKV
                     QYRLSLKNASSGTINLLCEYGATPSFDFTVEPNWSVNTILYNNVDVTTNLLNGVFTVP
                     AITANGLLSVSFINASSGAPELINSRIKVYSTNSEIIVEGTTNDETVSLYTLNGTHLQ
                     TLKSQGERISIPASRDAVYIVKTESKTFKVMM"
     gene            complement(31099..>31279)
                     /locus_tag="PKH58_02740"
     CDS             complement(31099..>31279)
                     /locus_tag="PKH58_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87975.1"
                     /translation="HNEDLPELIQASRKLLYKLDSLINLFDGTEFEAAYLNARRIVNS
                     GSRKAPASEEAPSAN"
BASE COUNT         8883 a         6098 c         6592 g         9506 t
ORIGIN      
        1 aaaatcaccc caaaagttca acaaaacttt tggggtgatt ttttagggaa agtggagatt
       61 accggactcg aaccggtcac ctcgacactg ccagtgtcgc gctctagcca gatgagctaa
      121 accccctttt ctaatgcgtt gcaaagataa acatttttgg aaataaattc gtatttttgt
      181 gtgaaaataa aatcatattg aaatgtttat atacaacacc actttccttg taaccgaccg
      241 gcgctttaat ccctggttca aatggctcaa agaggaacac atcccgaata tgctcaattc
      301 cggatatttc ttaaaaccac agatagctaa agttctgact gccgaaagtg atcaggaggg
      361 aatgtctgtc tcggtgcaat tcattgtaaa tgatcttgag aatctgcaaa actggataga
      421 taataacgaa ataagattac agaatgatct ttcattacgg tttggggcag aggtgctttc
      481 tttttcaacc attttagaac tgatggattg ataatggcga aagatcagat aatacttggc
      541 atagaccctg gtacaacggt aatgggatat ggaattatcc gggtaaaaga taataaagcc
      601 gaaatgataa cgatgggtgt gctggagcta aagaaatatt cggatcatta tcagaaatta
      661 caacgtattt ttgcccggac actttcactt atcgatgaat atcatccggt atgtcttgcc
      721 atcgaagcgc ctttctttgg aaaaaatgtg caatcaatgc tgaaactcgg gcgggcgcag
      781 ggagcagcca ttgctgctgc tttataccgc gatttacctg tgaatgaata tgccccgttg
      841 aaaattaaaa tgtcaataac aggaaacggt cgtgctgcca aggaacaggt ggctgatatg
      901 ttgcggcgat atctgcgcat tccacaggaa aatatgctgc ctcagctgga tgcaaccgac
      961 ggcctggctg ccgcgtactg tcattttctg cagtcaggta aaccaactgt tgatagtgaa
     1021 tacaaaagtt ggaaagatta tatcagtcag aactccagca gggtaaaaaa gtaaaataac
     1081 ccatcgcggg ttattacttt cgttcttttt gaaaatgttc taccggttaa gatcgatgac
     1141 atctaaaagt ccgttttcag gcatatccaa cggtaattcc caaaacatta atccgcccag
     1201 accttttttc tttacataat cgcttttcgc tttcaaagat tgtttgtcat cccctgttac
     1261 aaatatcatt ttctcgtcgt tgtaccaatg tgcagcctgt gctttttcgt cccagtaata
     1321 cttatagcca ttactttcag taaatatatt cacattcatt ttataatcgt gtgagcccag
     1381 aaatttcccc ggttgataga gaccattgtt tatattcgga acattttccc atgaacgggt
     1441 gtagaaagtt gccccgatta taactttgtg aagaggaaat cctatttttt cgagataact
     1501 gatagccttg tctgcagatt cctcattttc ccatgtgctg aataaaggtg tcatgtgtcc
     1561 ggtggtggta gcgtatccgc ctaccaggtc gtaagacatc aggtttacaa aatcaatata
     1621 aggattaatg gctttccagt cgatggcttt ctccatatac tctataaaac caccagaagc
     1681 aaagctgata atttttctct tcggaagtgt tttccgaagt agtgccactg ctttggtaaa
     1741 attattctta tcatccatca aatactgatg tccgggaaat cccttcacca ccggatattc
     1801 ccagtcaaag tcaaatccgt ccagattata ggttttcaaa aagtctctga ctgaacttac
     1861 gaaccgtttt gttttctgcg ggtcattaaa tacgttggaa cacaactcgc atccacccca
     1921 gcctcccaga gctatcatcg ttttaagatg cgggtatttt tgttttagtt ttatcagttc
     1981 ctttagtacg gtgagatctt tctgacgggt aacaccaaaa tatccgtcac tatttacatg
     2041 cccgaaactg taaatgacat gagtaatacg gctgtagtca tacatcatca atgatgcgct
     2101 atcggtagaa tagtaggcta ccagtttata cttacgctct gaagggttct tttgtccatg
     2161 gagtgctgcc gaaaatataa gaaccactaa actaattaga aatgcagtct ttttcataat
     2221 tattaatgga tgtttttact gatgaataaa tggtctgtta ggatgaattt caatcttgcg
     2281 aaatatcgta aatgaaaaga atattcgttt tgtttactga aatgattttt taaaaaagat
     2341 catttatgcg ttggtgaaat gcagttgcct gcaaacagct atgcttttcc ttcctttttt
     2401 cctccccgta tccagctgag cataaatgtt acttcctgtt gtgacagaac actactgagt
     2461 aattcataca ctgaaccgtt cagattgttt ttttcgagta aactgactgc tctctgtaat
     2521 ttttcaggac tgataaaccg ctccactttc aaaactcctt tctctaccag ctctgaaagg
     2581 tgtttggtaa tggttccttc cgtgaggttt cgttgtttag ctatttcagg tatagtattg
     2641 tcttctagat aaagttcaag ggaaacacgt acagtgtcaa ttttgggttt tttctccttc
     2701 ttttgagccg tttttttagt tccctttgct tccagtaagg acatttccga agacaaatta
     2761 ttttcgatgc aatatgtgca tatgcagttc agaaaatctt ctccgtattt atcaaccttt
     2821 ggttttccaa aacccgatat cttcaaaaga tctctttcgg tttgaggcag ataatccgcc
     2881 atttcggtta gcgatttaac tgtggcaatc agataaatcg gtaatccggt gcggtcgctt
     2941 atctcgttgc gaatatgaaa caactgataa agcagttgag gatgccgtga actgattttt
     3001 tggttcgaat tcgttttaga atatgaattg tgactgaaca gaggcaatac aaagttatgc
     3061 cggagttcaa aatatttctc cggattgaat ccgttttgga ttccttttat cagatgtttt
     3121 ttttgttcga tgaaattata gatatcttcc agaaaatcat catactcatt cgcgttctga
     3181 cggttatcgg tagaggctga tgtttggcgg agcgtatctg tcagaatgct cagatgttcc
     3241 gaaaaatatc cgtttgcagc ctgaagacgg ttctgaagta attcttcatc caaaggtttg
     3301 acagagttga tttgtatcaa ttgtgattga aaacgggatg ccacctgctg aagcgcttcc
     3361 gtttgtttga taatgttttg gataaacggc agggtgtcac tgttgaatga actttcnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggaa attgagtgaa ggtgccaagc
     3541 acttcttcgt cgatttgaag ggtggtttcg ttggctttgt aacggacgtt atgccatacc
     3601 ataggactta cttcgatctc ctgatcgtca tcgggacatt tcacaatgat tgtttcatct
     3661 tcgaaagctg ttattttacc aatctttcca ttgaaaaatc tttttggagt ttctgtgtcg
     3721 ttcttaatga acatgacttt agcgcccact ttaagttcca gtttttcctc aatggggtag
     3781 agcttttccg gaaaatcgcc tttcacctgt gcttcgaaat atcttgattt attcttcagg
     3841 gcggcaagtt cctctgaatt gatcctgtcg gctttataat tgtgtgttgt gaggatgata
     3901 tacgtgtcat cttttggtgg aacaaaaagc ggattgtatc tgctttgaag catagacata
     3961 cccttttcag ataggttgtt gtttctcact tcactgagta agctgataaa tgacatgtcg
     4021 cgctggcgga aaatcttttc aaattcaata taaacaggag gtttctcctg tataacgcga
     4081 ctgtgaaaaa aatagatgct ttcgtagtag ggcgaaagga gtttccattc ttcggattgg
     4141 gctacaggtg aaagctggta catgtcaccg atgaagatca tctgcactcc gccaaaaggt
     4201 tcgttgttgc gataacggat atgacgcagc aaggtatcga cagcatccag taaatcggcc
     4261 cgcaccatgc ttatttcatc aataaccatc agttccactt cccggattac tttccgccga
     4321 tggctttgca tttttagttt gctgatcaga tctttctttc cttcgggggt agggatgaat
     4381 ggtgtgaagg gtagttggaa gaatgagtgg atggttgtgc caccggcatt gatggctgcc
     4441 acacccgttg gagcgactac ggcaatctgt ttgccggtgt ttttccgaag ctcatgcaga
     4501 aacgtggttt taccggttcc cgcttttcct gtcaggaaaa tattgcagtt ggtaagttcg
     4561 gcaaactcac gggcaaagtc atgtaattca gaagtaatgt tcatggtctg tatttcaatc
     4621 agatacaaat gtacaaaaaa tatcggatta attaacagta caaacttgta ttatgatcgt
     4681 gcgactgatg ctttttaagt gtcatattgc tgctctgaat gacaaaaatt atagctgtaa
     4741 tcatttaaaa taaacaatcc gactgtgtca catcaggact tgcagtcgga ttgtatatta
     4801 gtttaatggt gaaatcagtt aatttcgaac aaagcttcat cctgcattac agacgcaccc
     4861 ggtgcaacgc atactttcac aatagtaccg cttttttcag ccaggatgtt gttctccatt
     4921 ttcatggatt ccataatcat gagcacatca ccttcgttga acgaacttcc tgcttcgacg
     4981 ttgattttta cgacactgcc cggaagcgga gcattgatag ttttcgaact gggtttagca
     5041 gtgataggca ttggagccag tttttcagca actttgtttt cacccggttt gttttgtact
     5101 tctttgcgaa ccaatatcgg ggttttcgga actttttgtt ccaactgatg aatctttact
     5161 tcataaggat taccatttac ctccagttcg gcaatttcat catcgatgcc ttttacgaca
     5221 acattatatt ctttttcgtt tattgtaaat ttatattttc tcatggtgag ttattttatt
     5281 tttgatttca cattaataaa ttaccgttgg aagttgttga tgctgtaaat cttggaactc
     5341 cagggagaat aacgtttctg aattctttcg atggtgatca cattgctttc caggtcgtga
     5401 acattttgag tgagataaag atgcaaggcc agaaaagcgg caacttcttc atcgttgccc
     5461 ggtgcacttt cagctttaac tacgttatca accgcaggtt gagctttata tacctgtttt
     5521 tctgccaatg cttctttctg gtctttattc ttctttatta aaagaaaaac aactacaacc
     5581 agagctactg ctaaaaccgc taatattgca actaacatat tatttctatt tgagttatta
     5641 atgaatatat cagactacaa aggcatattt gaatgcttgc gtggcggatt caatactttc
     5701 ttggtctgca aagcctcaaa agcacgaatt acgcggaaac gtgtgttacg tggttcgatg
     5761 atatcgtcga tgtagccata tgctgctgct tcataaggat tagcaaattt ctctttgtat
     5821 tcgagctcct tttctgtaac atatttggct ttttcgtcgg gatcggtaat accggcaatg
     5881 gtacgtcctt ccagaacttc aattgcacct gctgcaccca taactgcaat ttctgctgtt
     5941 ggccatgcat agttgaagtc accgcgaagt tgcttgcaac tcatcacatc atgcgcacca
     6001 ccgtacgatt tacgaatagt aacagtaact ttaggaatgg tagcttcgcc atatgcaaac
     6061 aacagtttag ctccgttggt gatgatacct gcatactcct gacctgaacc gggaaggaat
     6121 cctggtacgt caacaagagt aagaaccgga atgttgaaag catcgcagaa gcgaacgaaa
     6181 cgggcggcct tgcgtgaagc atccacatcg agcacacctg cgaggaagtt tggctggttg
     6241 gctacgatac ctgttgatgc accgttgaaa cgggcaaaac ctacgatgat gttacgggca
     6301 taatgacggt gtacttcgag gaattcgcca ttgtcaacaa tgctgtgaat gacatccttc
     6361 atgtcgtaag gatgatttgg attgtcgggg ataatgctgt tgagcgaatc ttccagacgg
     6421 tcgatcgggt cattggtagg atatactggt ggttcttcca ggttgttttg tggcatgtag
     6481 gagatcagct tgcggatcag catggcacct tctttctcgt caacaagttt gaagtgcgta
     6541 acacccgatt tgcgcgcgtg aacatcagct ccacccaaat cttcagttga gatatcttct
     6601 ccggttactg acttaactac tttaggacct gtaacgaaca tgtagctgtt gtcttcggtc
     6661 atcaggataa agtcggtgag agccggtgaa taaacggcac cccctgcaca cggaccataa
     6721 atggctgaga tttgaggaac aacaccagaa gccagaatgt tgcgctggaa aatttcggca
     6781 tagccagcca gagaagtaac accttcctga atacgtgcac cacccgagtc gttgatacca
     6841 ataacaggag cacccatttt catggccata tccattactt tacatatttt ctgagccatg
     6901 gtttcggaaa gcgaacctcc gaatacggtg aaatcctgag caaaaacaaa aacaatacgt
     6961 ccgtctattg ttccgtgacc ggtaacaacg ccgtcgccca tatatttttc tttttcgaga
     7021 ccgaagtttg tacaacgatg cgttacaaac atgtcgagtt cttcgaaact tccatcgtcg
     7081 agaagcatat caattctttc acgtgctgtc agtttgcctt ttttatgctg cgaatctatt
     7141 cttttttcgc caccgcccag tttggcctgc acacgtttgt caataaggtc ttgaaccttt
     7201 tcctggtttt tcatgtgttt tgttgtttaa atatttatta ttttgttttt ttatgaaagt
     7261 tcagttagca gcaagagttt ccgggtgctt tgtttgggtt ttcgcaaagt tcagtaagaa
     7321 ctccgatagt tgattttgga tgcaaaaatg caatattcag tccttcagcg cctttacgcg
     7381 ggttggcatc gatcagacga attccttttt cttcagcttc tttcaatgca ttagccagtc
     7441 cttcaacggc aaatgctaaa tgatgaatgc cttctcctct tttttcgatg aatttaccaa
     7501 ctgcaccttc agggtcggta gattccagga gttcgatttt ggtttgtcct acctgcaaaa
     7561 aagcagtttt tactttttgg tcagctactt cttctacggc gtagcatttc agacccagga
     7621 tgttctcgta aaatggtaat gcctcttcaa tgctgttcac agcaatacct aaatgctcga
     7681 tatgagttac tttcataagg cgaaaatatt taattttaat taataattaa ctgaaaagac
     7741 aattaaatac agaaaaaatt cagtatttaa ttgccccgca aatttactaa atattattta
     7801 catacatgat caatgattga cttaaatttt tgagattcta tgcaaataat attactgatt
     7861 ttgatttgct gaaatcgacg aatgatgaaa gtgcaaggcg ggaaaaatgc atcagtgaca
     7921 acataaatat aattatttgt tgtcactgtt gttgtgttaa aagaaatgtg tgctggagga
     7981 tgttctttct attgccatac cgaatttctt acaagtcttc aggaaaagat gcagatcagc
     8041 ccattttggc tttgaaggca agggcgtaaa gtttcatttg tttcaccatg gccagcagtc
     8101 cgttggaacg ggtgggagag aggtgctctt tgaggccgat cttttcgatg aaatagaggt
     8161 cggtgtcaag tatttcctcg ggagtatggt ccgaaagcac ctgaataagc atggttacaa
     8221 tgcctttgac gagtacggca tcgctttcgc cctgataaac aactttaccg tcaatcagtt
     8281 cggcgtttaa ccatacgcga ctctgacatc cttcaatgat gttttgaggt attttatatt
     8341 tatcgtccag tggttgcagg gtattgccca agtcaatcag aagggcgtat ttatccatcc
     8401 agtcgtcaaa ctggttgaat tcttctataa tttcgttttg agtttcgttg attgtcatat
     8461 aaagaagctg ccccctatcc ccctgaaggg ggaattcgga gcgtagatta ttgattatat
     8521 taatttgagt tcagaaattc atatttaata gtttgagggc ttcacatgtg ctcattgaat
     8581 gcactaaatt cccccttcag agcctgtccc gatttatcgg gagggctagg ggttatccca
     8641 gcattccgac cactcttttt aatgcattaa taaatatttc cacttcttct ttcgtattat
     8701 aaaaggagaa cgatgcacgt acagttcccg gtatcccgag gtagtcgatg agcggctgag
     8761 cacaatgatg accggtacgc acagctattc ccagcttgtc gagcagcatg ccgatatcgt
     8821 agggatgaat atttcccact aaaaaggaaa ttactgcgct tttatgttcc gatgttccga
     8881 atattcgcat tccatcaatt ttcatcagtt ctgcggttgc ataagcggtc agttcatttt
     8941 cgtatgcggc aatcttcttt attccgatgt tttccacata tttaagcgct tccgctaatg
     9001 ccgtagagcc gatgtagtcg ggtgtaccgg cttcgaattt gaaaggcagc tcattgtacg
     9061 tggttttttc aaatgtcacg gtttgaatca tttcgccacc tccctgatag ggtggtattc
     9121 gttcgagcca ttcccgtttg ccgtaaagta ctccaatgcc ggtgggagca taaattttat
     9181 gtccagaaaa aacatagaag tcggcatcca gctcctgcac atcaacctga atatgcggca
     9241 cagcctgtgc gccgtcgatt agcaccggga tattatgcaa atgagctgtt tggattattt
     9301 cttttaccgg attgacagtg ccgagtacat tggatacctg tgtcacggca accagccggg
     9361 tattctcatt tattaaatct ttgaaaatat ccagtctcag ttcacctttt tcattgatgg
     9421 gaataacgcg gagtttcata ccttttcgtt cgcagagcat ttgccaggga acgacgttgg
     9481 agtgatgttc catcatcgaa atgatgattt catctcctgc ccggcagcaa tattcgccaa
     9541 acgaaaatgc cacaagattg atggcttcgg tagttccgcg ggtaaatata acttcatccg
     9601 acgaagctgt attgatgaat cgggcaattt gcctgcgggc ttcttcgtgt gcttcagtgg
     9661 ctttctggct caggtagtgc acgccacggt ggatattggc attcaggtgt ttatatccaa
     9721 aattaatccg atcgagcaca gcctgtggct tctgtgtggt tgctgcattg tcgaaataga
     9781 tgagtggctt gttgtaaacc cgttccatta aaatcggaaa atcagccctt atattgttta
     9841 tttcctgttc agtcatttga gggttagatc cagaatgttt agattggcaa aattaatgat
     9901 tttttctgag ataagattaa ttgaaattgg agtactgaga gattataagt catttatatt
     9961 aagtttatct gtcatgcgaa acaacatatt attcttccca aattgacctc agtgtgtaag
    10021 gtccatccgg aaagacggca aacaacgctt tggcgttacg gtggttacgg tcaagtttca
    10081 tgatttgttc catatctttt tcgtcgagtt ggatgtattc agcaccccga ttctcttgta
    10141 ttcttgttgg attgaccgat ttggggatga caactgtgct gcgctggatg ccccaggcaa
    10201 gtgcaacctg cgcttctgtg cagtgatttt taagggctat ttctttaacg acgggatgaa
    10261 gcaaaatgga attttcgtta tcggtcaggt gatggctgcc gagcggagaa taggcagtga
    10321 caagtatgtt gttatcctga caaaacctga ccagctcgtt ttgctgcaga tagggatgaa
    10381 gttcaatctg gttaacttcg ggttttattt ctgagtttgc aataagattc tttagtttgg
    10441 gaatattgaa attggaaaca ccaatatgtt ttgttaatcc cgatttatgt actgcctcca
    10501 tacctttcca ggttgatgtg agcggtattt cttccagaga aacaaggtcg ccgacactca
    10561 gggcttgttc ctgtcggaag gccagcggcc agtgaataag atacaagtca agataatcga
    10621 gtttcagatt ttcgagcgat atcccgatgg ccttttccac atcctgaggc gcgtggaaat
    10681 tgttccagag tttggaggtg ataaacatct cctcacggct aaccagtccg gtagaaaaag
    10741 catactgaag ggcttcacct atttcctttt catttttata gatgtacgcg caatcaatat
    10801 gtctgtatcc agcaccgata gcttcgatta cggcattgta aacttcactg gatttcgaaa
    10861 gccaggtgcc caacccgatt tgcggaaaac ggtcgccgtt aataaaatgc atttttttca
    10921 tgttgttgag aatttattct tcaatggtta taacgtaaag caggataaga actacaaatg
    10981 tacaataata aattaagttt tattgatata caatgattat cagtcttgtg tctgaaataa
    11041 aatacggaat ttgttttcaa caatataatt tctaaatttg tacatttgtt ttcatctcat
    11101 ttacgatcaa tcataactct taatctacat aatatgaaga aactattttt atccttaact
    11161 ctttttttat tcggatcatc tgttttcaat cagacatttg ccggtaaatt caatattatt
    11221 cccgagcctg tagcccaagt ggagaagccg ggaaatttta ccttgcctaa gaatgtaaat
    11281 attcttcgtc cggctggtaa tgaaaatgac ataactgtca attttctgaa agaaaaatta
    11341 tctaaagcta ccggcagtaa agttactgta agatcgtccg gtaaatcgga tattcaattg
    11401 atcttgaata aggtaaacga cacgatgata ggtgaagaag gatacatttt atctgtcagg
    11461 tctaaaaaaa tcactatcag ggctaataaa ccgtcaggat tattttatgg tgcacagacc
    11521 ttaattcaac tttttccggc gcaaatagaa agcgaagaag tggtgaataa cgttgaatgg
    11581 aaaatacccg ctgtagaaat tggtgattac ccccgtgtag catggcgagg tctgatgctg
    11641 gatgtttcac ggcatttctt cactgttgat gaagtaaagc agtatatcga tgctatggta
    11701 cgctataaat acaatatgct tcactggcat ctgaccgatg atgaagggtg gcggattgag
    11761 ataaaatccc tgccgaaact aactgaaatc ggtgcgtggc gggtagaaaa aatcggtaat
    11821 tttggtgaat tttcggctcc gttacccgat gagcctaaaa cttacggagg tttttataca
    11881 caggaacaga ttcgtgaaat tgttcggtat gctgcagatc gctttgttaa tatcatacct
    11941 gaaattgatg tacccggaca tagcctcgct gccattgctt cgtatcccga gctttcgtgt
    12001 accgaaggtg ctgataagta taatgttcgt gccggtgaac agtttatgga ctggtcgcac
    12061 ggagcaccac ccattgcctt tattgataac accctgtgtc cggccaacga aaaagtttac
    12121 gatttcatgg ataaagtgat gacagaggtg gcacaattat tcccgtatcc gtatattcac
    12181 gttggcggtg atgaagcgcc ccacaatttc tggcaaaaaa gtgcacaggt gcaggaactg
    12241 atgaaacggg agggactgac cacgtttcct caggttcaat cgtattttga gaaaagactt
    12301 gagacaattg ttttggcaaa aggtaaaaaa atgatgggtt gggacgaaat tatggagggt
    12361 ggcattacac ccactactgc tctgatgagc tggcgcggtg tcaagtatgg aattgaagcc
    12421 tcgcgttcag gtcattatgt ggtaatgagc cctaccactt ttgcatatat tgattatatg
    12481 cagggggatg tttgtaccga accgccggtt tacagttcgc tccgcctgaa taaaacctat
    12541 cagtttgatc cggtaccacc cgaagccgat gcaagatata ttttgggcgg acaggctaat
    12601 ctctggacag aacaggttta caatatccgt caggctgaat acatgacctg gccacgtggt
    12661 tttgccattg ccgaatcact ttggtctccc aaagacaata aaaactggga aaacttcgtt
    12721 agcaaaaccg aaaatcattt tcatcggctg gattatgccg aaaccaagta ttcaccggcc
    12781 atatacgatc ccattgtaaa tgtgatgaaa aaggatgata aatactatgt ggaactgaca
    12841 actgaaattg gcgggttgga tatttacacc agtttcgata attctactcc tgactgtttt
    12901 tatcagcgct acaccgaacc cttgtcgttg cccaaagatg cccggttgat gaagataata
    12961 acctatagag gcaaagtccc tgtgggcaga ctgatgacca taccgataga tgatctgaaa
    13021 aagcgggcaa aataatttac tagagaacag acggaattta gctatttcgt ctgttttttt
    13081 attggatggt gacttttttc cggatgacta ttctgtcctt taatttcagg aagaaatctt
    13141 caaaataacg ttttgaaata gccgaaacac ccacaacgac aattaaagtc acgggataat
    13201 acagcagtga acctgtcagc agatccagtt ttgagagttg tttttgcagt accagcatca
    13261 atccaaagat tattggcata tgatacatat agataccgta tgagatttcg cccaggtaag
    13321 aaaaaaacct tttgcgcagt ttgaagatgc tgtgatcaat aagcgaaacg ccaatgatga
    13381 ggtacaaaaa caaaaaacag atgaaaagct gcgaaaaaac cggcgtttta aagaatatat
    13441 tccaaacgga attgtcaata ttgcgattga aaaccagaaa gataaccagc aaactatata
    13501 tcgctatctg aactggaatt ttatatattt ttagtcccga cagtgaatgg tttctgttgt
    13561 agataaaata tgctcccaga ccacctacag ccattgcttc aaactgaaat atatccacga
    13621 gataagaaaa taaattattt tccacgatat aaaagctggt gataatcaaa aatgatttga
    13681 tcgcgatgat cgaaattaat atcaacagca aatatttttt tgcaaattta aagagtggcg
    13741 cccagatcag ataaaaaact tcctcgatac ctatggacca gagaggctca agtaaatgat
    13801 gtccgaaaaa gtaagtaacc agcccgggaa gaaaaaacag gaagtaatgc caggtttgcc
    13861 cgaaagtata aggcattaca tagttcagcc cgaaaatatt aataagagca ggcagaagca
    13921 gggtgccgaa aataacaagc agaaagtaca gtggccagat gcgaagtact cttttgaggt
    13981 aaaatcgttt gatactaaca gttccgttct gtccgttttc tttcaacagc aaataggtga
    14041 ttaaaaatcc gcttagcaca aaaaagaagg taacggcgat actgccatcc ttgaagagcc
    14101 ccagccagtt caggttcggt agtccatttt taaaacggat tgtttcggaa tgatgaagta
    14161 ctaccaggag cgcagcaaaa aaacgaagcg aatttaatcc gttgaagaaa gaaatatttt
    14221 tatagtttgt catttatttt gcgtgcatcc tgttcagccc gcaaaattaa ccaattttgc
    14281 ttataaatga gatgttggtt agaaaaaatc caggctaact ttcagtttat attggttaac
    14341 ttgatgtgta aatctcttag tacggaattc agaccgtagc agagagatgc aatttattaa
    14401 aattaaccaa tcatagcggt agcaaaacgg aaaaaatgta gtacttttgt gtgattttcc
    14461 ccaaaaactg ttttttcaat acagaactta gacgtatgaa taaaaccatt ctgaattcac
    14521 tcaccccgca tttagtagca ttattgcttt ttatagttat ttcattcagc tattttacct
    14581 ctgtactgga aggaaaaaaa ctgattgcac acgataccca gagctggata gcagactcaa
    14641 aggagacagt agattataat gaatcgcatg atgatgtcac cctgtggact aattcattgt
    14701 tcggaggtat gccaaactat cagatttcga tggaacagcc gaataatgta ttgcaatatg
    14761 tagaaaaatt ggtgatggaa tttccccggc cggtttcaaa tctgctgctt tacctcatct
    14821 gcttctatat cttactgctg gcattcggat ttaagccctg gatggcaata ataggctcca
    14881 tcggttttac ttttgcttcc tacaatttta tcatcatagc cgccggtcac aactccaaag
    14941 ccatcaccat tgcctatatg gcaccactta ttggcgcggt atttctggct ttccgccgga
    15001 aaaggctgct gggtggaatt cttaccgcat tcttcttaag cttggctatc cgggcaaacc
    15061 acatccagat tatatattat acactgattg tactcctgat atttgctgtt gtagaattca
    15121 tctacagtat tcgcgaaaaa gaaataaaag aattcctgaa aacggctgtt gtgatgattg
    15181 ctgcagctct tttggctgta gggatgaatg ctacatcact gctcacaaca tatgaataca
    15241 gtcagtatac catgcgggga aaatcaaacg gactaactac agctgtgcaa aattcaccca
    15301 atgcagtaaa taccggtcac ggacttgata aagattatat tacccagtgg agttacggag
    15361 tggatgaaac gatgaccctg ctcatcccca atttcaaagg aggagccagc ggaggaaaac
    15421 tgagcatgaa cagcgagaca gccaagtatc tgaaaaatat gaacgttcct gacagtcaga
    15481 cgcagcagat tgtaagccag atgccgctct attggggaac gcagcccgga acttcagggc
    15541 cggtatatct tggcgcaata ataattttcc tgtttgttct gagcctgttt gtagtcgaca
    15601 aacgtacgct ttggtggtta attcctgtaa taattcttac actgatgttg tcgtggggtc
    15661 gtaacttcat gtggctgaca aatatcttta ttgattattt ccccatgtac aataaattcc
    15721 ggacggtttc catgacactc gttgcaaccg gtttcggaat tacgctgctt gcatttctgg
    15781 ctctgaaaga aattttcgat gagaatgtaa ataaagaaaa actgaccaaa tcagttgttc
    15841 tcagtatgag tattacggct ggtatctgtc tgatatttgc gttattacct tcactggctg
    15901 gtggttttgt ttcaccacag gatgctcagt tctcgggcga ttattctttc ctgaaagaaa
    15961 cgcttccgct cgacagaaaa gcgatgcttc gtgccgacgc atggcgttcg ctggctttca
    16021 tcctactcgg ggcagttgtt atcctggctt atatcaaagg atttctgaag aaaaacgttg
    16081 cattggtact tcttggattt tttattctga ttgatttatt cccggtggca aaacgttatc
    16141 tgaatgacgg caacttcgag aacaaacgtc cggctcaact tatacagcct actcaggccg
    16201 atcagattgt actccaggat aagtcgcaat atcgggtgtt ggatgctacg gtagatatat
    16261 tcagtgatgc cacaccatcc tattttcata agaatatcgg aggatatcat gccgctaaac
    16321 ttcgtcgtta tcaggaattg attgaaaatc aaattcaacc ggaaatcgga caactgtttg
    16381 gcgcgtttgg taaagctaca actgctgaat ccattttacc caccctcgaa tcgctgggag
    16441 tgctcaatat gttgaattta aaatacatca tttataataa agaatcggca cctctcgtta
    16501 atccctataa caatgggaat gcctggtttg tgcagaaaat acgtattgca gccaatgccg
    16561 atgatgaaat aaaactggtg ggcgaaattg acacccggca cgaactggta gtcgataaat
    16621 cgctggcttc gatatttccg acaacattaa ctcctgacac tacagctaca atcgaactgg
    16681 caagttatgc acccaatcat ttggtgtata aatttaactc aaccacagat caggtggcag
    16741 tattctcgga aatttattac gataaaggat ggaaggccac aatcaatgga aaagaagttc
    16801 cttatacccg ggtaaattac ctgttgcggg ctatgccgtt aaaggcaggc aactatgaaa
    16861 tagattttcg tttcgatccg caatcctaca aagcaggtaa tatgctggct atcgtttctt
    16921 ctgcattatt gctgatagcg attgcattac tcgcattctt ttaccagaga aaaaggaaaa
    16981 caacaacacc gaaacccggg aagaattaat tacaaaaaaa acaaacctcc ggatgatatc
    17041 cgataggttt tcagaataat atattaacga tattatgtct acaaaaaaat acaaaaaaag
    17101 aacattccga tttcgcaatg ctaatttgac agcaacattc agtatgtcgc tggtgctttt
    17161 tctggttggt ttactgacac tgaccctctt tctggcgcgt gatatgaccc gctatgtaaa
    17221 agaaaatctg aacctctcta tcgttctgag tgatgaaata accccgcgac aaactgaaag
    17281 gattaagaaa tttctggaga aatcggatta tgccaaatca gtggagttga tttcgaaaga
    17341 acaggcactt caggaacata tacaggattt gggcgaaaat ccggaagaat ttctgggttt
    17401 caatcccctg ctggcatcgc ttgaggttaa actgaacgaa aactacgcca ataccgacag
    17461 tgtcgcaaaa attgaaaaga aactttcaaa gtacaaggaa attcaaaagg tttcctatca
    17521 gaaagatatg atttcgatgg taaatgaaaa catcaaaaaa atcagtatca tcctgctggg
    17581 tatcgccgct attctgatgc tcatttcatt tgccctgatc aacaatacgg ttcggctcag
    17641 catttattcc aaccgttttc tgatcaacac catgaagctt gtgggtgcta cacactggtt
    17701 tatccgtaag ccttatgtca gacgaagtgt gctgaacgga ataattgcag ctattattgc
    17761 actcggatta cttaccggac tggttttcta tttccagaat gaatttggtt tgcagtctca
    17821 aatgatcacc cgcgataccg aattgattgt tgcagctgtt gttatcgttc ttggcattct
    17881 acttactgcc atttcatcct actttgcagt aggccgatat ctcagaatgc ggtctgacga
    17941 tatgtattat atataaagat taagtggatg aataataaaa ctgttctgat acttatttca
    18001 tgtgttattt ctttgtaacg agatattact gaatcaataa actcacacat aaaatggaaa
    18061 ataaaaaatt cggactttcc aagataaact ttatcctgat tgcagttgca cttctggtaa
    18121 taataatcgg atttctttta atgaccggaa agccttcggg agttgaattc aatcccgata
    18181 tattcggcac ccgccggata atagtgggac caatgatttc gtttatcggc tttatttcta
    18241 tgattgccgg tatcctttac accccgaaaa acaagaatta agaatgaacg taattcaagc
    18301 gatagtcctt gccattgtgg aaggattaac tgaattttta ccggtgtcat ccaccggtca
    18361 tatggctttg gtttccgcgc tgatgggtat tgagcaggag agcttcacca aactgtttat
    18421 cgaaacaatt cagtttggaa ctattctttc tgttttggtg ctttactgga aaaaatttat
    18481 tgatttcaag ggttttcagt tttatataaa gctcattatc gcatttcttc ctgctgcagt
    18541 atttggcgtg atttttaaga aacatattga tgcgctgatc ggaacgcccc tttttatcgc
    18601 ctgcactttg ttttttggcg gaataatact tctgtttgtc gataattggt tcaaaaaccc
    18661 gaccattgac gatgaaaagc aggctaccaa caagaatgcg ttgattatag gactgttcca
    18721 ggtactggca gtcgtcctgc ccggtttaag caggagtgca gccactatca ttggtggtat
    18781 gcagcaaaag atgacacgta aagcggcagc tgagttttct ttcttcctgg cagtaccaac
    18841 tctggctgga gcatttgtaa aaagtttatg ggatgcattt caggaaacac ctgaaattct
    18901 aactcaacat aatatttctc tgcttgtact gggcaatgtg gttggcttca tagtggcggt
    18961 tatcgctatt aaagctttca tcagtttcct ggccaggcat ggatttcgtt tgttcgggta
    19021 ttatcgtatt ctggtaggga ttaccattat tttgctaatt ctgtttggcg taaatttgag
    19081 tgttatttaa taagttttac agggagcgat aatggatttt attgaaggag aggtattgta
    19141 tgtgaagaaa cccctgcact ggacatcgtt cgatgtagtg aataaagtgc gctggaagat
    19201 ttcacgaaaa ctaaataaga aaaaaattaa ggttggtcat gccggtacgc ttgatccgtt
    19261 agccacagga gtaatgatac tatgtacagg taaagcgacc aaacagattg aggatttcca
    19321 atatcagacg aaagaatatg cagctacact tgaactggga gcaacaacac catcgtttga
    19381 ccgggaacat gaagtggatg cccgttaccc gacagaccac attacccggg ggttaatcga
    19441 tgaagtgatt tcaaggttca aaggtgaaat ctggcaggtt cctccggtgt attccgcagt
    19501 gaaagtggat ggcaaaagag cttataatta tgcccgtgac gggcaggaag tagaactcaa
    19561 acctaaacta ctggtgattg atgagattga agtactgtcg tatgattttc cggtattagt
    19621 gattcgtgtt gtgtgcagta aaggcactta tataagggca ttggcgcgtg atatcggaga
    19681 agccctgcaa agtggagcac atcttattgc cctggaaaga acgcgcattg gagatattac
    19741 tctgactgat tgttgggaaa ttgatgattt gttgaaacaa attgacacgc ttgattgtta
    19801 aataatggtt gttaaccatt caaaaaatat tttctgatgt atcgaaagca cacggtatgt
    19861 caaattagta aatattctaa gaggcaatat caggtaatta agtgctaagg agctctgcaa
    19921 aattatttat tcagaaaaaa ataacgagaa actaaaaata cgaagtaaaa ttaagattat
    19981 gaaattatct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaaataac
    20101 gagaaactaa aaatacgaag taaaattaag attatgaaat tatctcagtt taagttcaaa
    20161 ttgccggatg aattaatagc tcaatatccg gctgtacaca gagacgaatc ccgtttaatg
    20221 gtcttacatc ggaaatctgg tgaaattgaa cataaggtgt tcaaggatgt acttgattac
    20281 tttaatgaaa acgacttatt tattttcaat gacacaaagg tttttccggc ccgtttgtac
    20341 ggcaataagg aaaaaacagg tgctcagata gaagtatttc tgctccgcga actgaacaga
    20401 gaaggcaggc tgtgggatgt gctcgtagaa cctgcccgta aaatccgtat tggtaataaa
    20461 ctttacttcg gtgaagacga ttcgattgtt gccgaggtga ttgacaatac cacttcccgt
    20521 ggacgcacac ttcgtttttt gttcgatggt actcatgagg aatttaaacg tgaactctat
    20581 aagctgggtc aaactccgct tccgcacaat atcacaagac ctgtagaacc cgatgatgaa
    20641 gaacgtttcc agacaatttt tgccgccaac gaaggtgctg tatcagcacc tgctgccgga
    20701 atgcatttca gccgggagtt gatgaaacgc atggaaatca aagggataaa cacagcattt
    20761 gtcacttcgc atatgagttt gggtaatttt agggaaattg atgtggaaga tcttaccaag
    20821 cacaagatgg attcggaaca gatgcaaatt actcccgaaa ctgctgacat tgttaacgca
    20881 gctcatgatg ctggtgaaaa tatatgcgca gtgggtgtga cggtgatgag agcactcgag
    20941 actgttgtag gaactgaagg acgtattaag ccgctggacg gatggaccaa taagttcatt
    21001 ttcccgcctt acgattttac agtagctaac tcgctgatta ctaatcttca cctaccgtat
    21061 tcaactttac tgatgcttac agctgctttt ggcgactatg atcatgttat gaatgcctat
    21121 gatgtggcga ttaaagaaaa ataccggttt ggagtttatg gcgatgcaat gcttgtaatc
    21181 taaacagaat ataaccttaa aaaagaaatt cgctggtaat tatcagcgaa tttctttttt
    21241 aatcaatttc catatccaga ttttttcgga gaacggctaa agccgggttg gctccaacca
    21301 ttttcttata aacttcctgt ggagacagat atgttttttg ttcttcttca ggtatgaaat
    21361 ctatctgaaa gcgtatcgaa ttatttccca gttccttccg caggtattcc agtacatgta
    21421 tctggctgat ctctttttcc tgaaatttat tgtcaagtaa caatccgatg atattgtcat
    21481 tctccaggct tggcaggtga tttagtaaaa agccccgggt aacctgatct ttctggaatt
    21541 gattggcaaa tattttccat ttttcaatca gttcctgact ggtaaattct ttatcggata
    21601 tattgtcatc agtatgtttt ttaattcctt ccgatgcagt ttcagttgat ttttctgatt
    21661 gaatatttga tatggagatc cggggaggag cttgtgaaac taccgttttg atttccgcag
    21721 ttttcttcgg aaccggattt cctgcataaa catcagtagt gtcaggttcg ctgacaatcg
    21781 tatcagccgg tgtagtctga acaactggct gaggttgagt aggaggcgcc tgcgtaacga
    21841 taacttgtgg ctttgaggtt atcacaggct gagtgattat agccggttca gaaacttcga
    21901 tcggttttaa aggaggttta tcaactgtca gatttttttt tttatcggac ataagctggc
    21961 ttatccttat cagagctatt tcaacgagta atcgtttgtt tttactgatt ctgtagttca
    22021 aatcgcagtc gttactgatt ttcagggcat caaagataaa ttccacaggg catgcctgag
    22081 cctgttgcct gtatcgctcg ccgatatctg tccctacttc gagcaactga atagtgcttg
    22141 catctttgct gacaagtaca tcccgcagat gcgaactcag acccgtaatg aaatgctgtg
    22201 cttcaaatcc tttattaagt acttcatcaa aaatcagcag tgcttctgaa acgttacctt
    22261 gcagaaatgc atttacaagt ttgaaataat attcatagtc cagcacgtta agattgtcaa
    22321 tcacattctg ataagtgatt gcattgccgc tgaaactgac cagctgatcg aaaatggata
    22381 atgcatcgcg catacctccg tcacttttct gcgcaatgat attcagtgca ctttcttcga
    22441 tggtgacatg ctcactcgat gcaacatatt tcagatggtt aatcgtatca ttgattgaaa
    22501 tccggttaaa atcataaatc tgacaacggg atagtatcgt cgggatgatt ttatgctttt
    22561 cggtagtagc caggatgaaa atggcatggg ccggtggttc ctccagtgtt ttcagaaaag
    22621 cattgaaagc gcccgaagat agcatatgga cttcatcgat gatatagacg ctgtaatttc
    22681 ctatctgagg cggaattcgc acctgatcgg tcaggcttcg gatatcatca acgccattat
    22741 tcgacgcggc atccagctcg tgaatgttat atgagcgctg ttcattgaaa gttttgcagg
    22801 attcgcactg attgcaggcc tcaaattctt ccgtcagatg ctcacagttg atggtttttg
    22861 caaaaattcg cgcacaagtt gtttttccca caccccgtgg tccgcagaac aaataggcat
    22921 gtgccagttt tttgcttttt atggcatttc tcagggtggt agttagtgcg tcctgcccca
    22981 caaccgattt aaaggtggat gggcggtatt ttcgggctga aacaacgaat gaatccatga
    23041 aaaataagat tgttttcttt gataaattta ttacaaatat aagctatttt tctgaaacag
    23101 caagttgtct gaatctgccg ttttttataa acatcgcata aaaacaaaaa aaagcttcgg
    23161 atggttacac ccgaagctta tcgataatta aggtttttat gtaaagataa tggtttatac
    23221 tttttgggca gttgtttctg agttttactt attcagacct ttttttattt tttttatttc
    23281 cctttgctgt cttttcaaac ttttgtttat ttccagctgt gaattttcgt catgcctggc
    23341 caggtaattg tattttgatt tatgtgcttc gttttcggtg aatggtaagt cagtgtcgag
    23401 gtataattgt acctgtggcg atgttttcag attttcgagt tttaatttag gcggaactga
    23461 ttttacaata aaccatacgc catccttttc gtaaatgtac acatcttttt tcaggtcgaa
    23521 ataagtatta tactccggaa aatagatatg tctggtttta gctctgtagc cattcgccgg
    23581 agcccattca ggtggtactg tttcaccatg tgcagggaat acaagcagaa ctgaaaagat
    23641 ggcaataaat attattatgt atttgttcgc tttcattgca tggattaaaa cctgattaac
    23701 tgtaaaaatc aggctttaat tttatttcaa acatcgtgcc aaaaaattac cctttgacaa
    23761 aataattgtc aaagggtaat ttctatttta aaatgaattg tcctttctgt tacagcagat
    23821 attgctcact gatcgattta tgttcctgta cgcggtgaat tgtttcggca aacatatcgg
    23881 caatagtcag gattttagct tttgagcatt tgttacaatc aaacggtatc gaatcagtaa
    23941 acgctatctc ggtaagtgca gagttcatga ttcttttgct ggcttcaccc gacattacac
    24001 catgtgaaac gattgctctg acacttaagg ctccattttc cagcatcaga tcggcagctt
    24061 tgcagagtgt accggcagta tccaccatgt catcaacaat aattacattt ttgcctgcaa
    24121 cttcaccaat gatccgcatt tcgcctacaa tgtttgcttt ttcacgggtt ttatggcata
    24181 aaaccatagg aacatccagg tgtttggagt aggagcctgc acgttttgaa ccgcccacgt
    24241 ccggggaagc gataaccaga ttttcaagct gtaaagactt gatataaggt ataaaaatgg
    24301 tggacccata caggtgatca acaggtacgt tgaagaaacc ctgtatctga tcagcatgaa
    24361 gatccatggt tattacacgg tcaacaccgg caatagtaag catatcggca ataagttttg
    24421 caccgatgga tacacgtggt ttgtcttttc tgtcctgccg tgcccatcca aagtaaggaa
    24481 ttactgcgat taccttgtaa gccgaagcac gtttggccgc gtctatcatc agcagaagtt
    24541 ccatcaggtt atcggaattc gggaaagtgg actgaaccag gtaaatgtac tgtccccgga
    24601 tagattcctc ataggaaact gaaaattctc catcggaaaa gtgctcgata ttcatcttac
    24661 caagaggcca atttaaactt ttacaaattt tttcggccag ataacggctg ttagtgcctg
    24721 taaaaacttt gaacagagat gaatcttctg acataaatta gatgatttga ggttggtgaa
    24781 attttttaca aaagtactca atttgacata aaacaggaaa gtttttgctc tgaattttca
    24841 tgttttattg ttttgatgcc atacaagact gtaaaataga taattaagaa ttgtgaaaaa
    24901 tgagctatac aaattcaaag ttttcgcaat cgtttgaaat taatttgata ttgttttatt
    24961 ataaaataaa tttacggatt tattatataa agtatttttg aataaaattt tctgcagggt
    25021 tcaaatatca agcgggctga ggcccgatat tctttaccct gatttaatat aactgtttat
    25081 ccgattatca aaaagcaaaa tttcaacaaa ataagaatat ccatgagaat acactttaaa
    25141 attccttact tcacaaaatg gggacagcga attctggtta gtggcgatgt ccccgagctg
    25201 ggagatggcg attacttaaa agcattaccc ctgcgatttc agggacatga agactgggta
    25261 aatgatgtgg agtgtagggt ggcggagaaa caggttgttc attataaata tttgatttat
    25321 gatgaaaata cgaacagctt tacagaagaa tgggggaatg accgggtaat tgaccagctt
    25381 acgctgaaaa cagaacatta tttctgttac gatacgtgga actccatcgg gagccatgag
    25441 aatgttttta tgacaagtcc gtttcaggaa gttcttctga aaggaaatac cacaaccagc
    25501 cctaacggaa agaataagaa attcacccat atatttaaag tgaaagcgcc gttgctacat
    25561 caccggcaga ctttatgcat tatcggcagc tgcggggaac tgggcgaatg gtcggtggct
    25621 gaaccattgc tgatggttaa ggacggtgac aactggacga ttaaaataga tctgagcaaa
    25681 cgaaccactg atgtaaatta taaatacgga gtttacgatg ccgatgaaaa acggtttatg
    25741 ttttttgaac aggggccgga tcgttatgct gctgtaatcg gatcgaaaaa caccattgta
    25801 caggtacatg atggttttgt gaggatgaat aatgactcct ggcggggagc gggagtgagt
    25861 cttccggttt tcagtatccg tacccgccgg agctttggtg tgggcgattt tgaagacatg
    25921 aaaatgtttg tggactgggc tgcaaaggtc aatatgaaac tgatacaggt gctgcctctg
    25981 aacgacacca acggaaccca taccgatgcc gatgtgctgc cttacgcggc aatctccgcc
    26041 tttgcgctca atcccctgtt tctgaatctg cccgcgatgg gcaaattacc cgaaaacaat
    26101 gccatcgggc aacaatacat acccaaacag gctgaactca acgcgtaccc gctgatatca
    26161 tttctcgaag tgattgattt taaaatgaaa tatgccagag agatgtatct ttatcaaaag
    26221 accgaattcc tgaacaaccc ggatttcatt cagtttttcg aaaagaattc ctactggctg
    26281 gttccgtacg ctgcttactg tgtgttgcgc gataagtttg gaacaaacga ttatcgcaac
    26341 tgggaagagt tcgcggttta cgatgccggg cagataagcg cgtttacgga taaaacccag
    26401 cctcatttcg atgaagtggg cgtcaactat tttattcagt accatcttca caaacagctt
    26461 tcggaagtag ctcagtacgc tcatcagcgg ggagtggttt tgaaaggcga tatccccatt
    26521 ggaataaaca aaaacagtgt ggacacctgg gtgtcgccgg aattgtttca catggatatg
    26581 aacgctggcg cgcctcccga tatgtttgcc gttaagggtc agaactggga actgcctacc
    26641 tataactggg agatgatgaa aagaaccggc tttggatggt ggaaaaaacg tttcgaacag
    26701 atgtcgaact acttcgacac attccgtatc gaccatattc tcggattttt ccgcatctgg
    26761 gaaataccca ccaatcaggt ggaaggaatt atgggacatc tcaatccatc cactccgata
    26821 tacctcaatg aatttggcga aaagggtatc tggtttgatt acaaccgttt ctgcaaaccg
    26881 tatattaccg atcagatatt gtgggatttc tttggagagg aagccgcatg ggtgaagaca
    26941 aactgcctga agatagagga tggctgggtg ttgcgcttaa aaccggagtt ccagagtcag
    27001 acttatgtgg aagagctttt taatcagggc gaaatttctg aacgggtgaa atggggacta
    27061 ttcgacctga tagcaaacgt gttgtttttc gaagtaaaag atagcaacgg tcagcagttt
    27121 tatccgagat acggcatgca cagtctgcgt tcatacattg aactggatca gcacaccaaa
    27181 aaaggactgg atgaacttta tattcattat ttctaccatc gtcaggatga aaactggtat
    27241 cactccggca tggaaaaact tccggcgctc aaacgttcca ccaatatgct catctgcgga
    27301 gaagatcttg gtatgatgac accctgtgta acggctgtaa tgaacgaact cggtatatta
    27361 agtctggaag tgcagcgtgc gcctaaaacc agcaagattg atttcttcca tcctgccgat
    27421 gctccctact tgtcggtggt aactccatcc acccacgata tgagtaccat tcgcggctgg
    27481 tgggaagaag accgcgcggt gacacaaaaa ttctacaaca cccagcttgg tcattgggga
    27541 gatgcacctt tcttctgcga gtggtggata agccgcgata ttatcctgca acatctttat
    27601 tcgcccgcca tgtgggcaat attccagatg caggatctgc tcggcatgtc tcccaaactg
    27661 cgacgtgaaa atccgcatga agaacgcatc aacgtgccct ccaactccat gagcagctgg
    27721 cgttaccgac tgcatctgaa catggaagac ctcttgcagg aagatgaatt caataatgaa
    27781 ctgagaaatt ttatcgtgca atcaggaaga taaattcaac gaactataaa caaagtaatc
    27841 ccctttacga catgaacgta aaggggatta ctgtttttaa tatgagttcg atataaaagg
    27901 aaactgtatc ttactgctgt atgagtgctt tcaagcggca aaacaagaat gtttttttaa
    27961 gtcatcgttc ctttgtacta tgtatggctt ctgcctaagc cgctgtttaa tgtaaagctc
    28021 agctttccat attctttttc aatatggaag tctggagact tccttttagc acgatgaagt
    28081 ttggaaactt cgccgaacaa ctacgccaac ctacgctcaa cagagttttt atttatgttt
    28141 tcccaaattt tcgtgcaact gtcaaataac tgtccttcgt atttagagtc taattccatt
    28201 cgttgtaaaa ccattaacaa ttcacgttgt tggctgtctt gtctgttagg taatatgtca
    28261 ataatcattt ttatgtgtct acgtattcgt ttgtcattgt tgttcataca actccataac
    28321 aaccaagcgg cacgccacga ataaggtttt ttgtcggaaa tggatagttt aataagttca
    28381 gggaaatcgt cttggtgcga tgctatgtaa gaaatcaaat cgtctttata catattagta
    28441 agagtatatt ccaaattagt ttctttatag tccttcattt taataagttt gtcggtcgtt
    28501 ttttcaatat ggctaataac accgaaatat gcgttacgct tttcctgtat acgccatgcc
    28561 gtagagttac tgcaaaacaa gattgttttt aagtcctcgt tcccacgtga tgatcgcgcg
    28621 ggagcaaagg ggtttaatat cagagataag acaaaaatga ttttacatca tcactttaaa
    28681 agtcttgctc tccgttttta cgatatatac cgcatccctg cttgcaggta tacttattct
    28741 ctctccctgt gatttcagtg tttgcaggtg tgttccgttc agggtgtaaa gacttactgt
    28801 ttcatcgttg gtagttcctt ctacaatgat ttctgaatta gtgctgtaca cttttatgcg
    28861 gctgttgatc agttccggag caccacttga agcgttgatg aaagagacac tcagcaaccc
    28921 gttagcagtg atggcaggta cggtgaatac tccgtttagt aagttagtgg ttacatccac
    28981 gttgttgtat aatatcgtat ttacactcca gtttggttct acagtgaaat caaatgatgg
    29041 agtagctcca tattcacaga gtaaattaat tgttccggat gatgcattct tcagtgaaag
    29101 acgatattga actttttgga acgttacact tagcgttgca tttgcattaa ttgcgggtgt
    29161 agtgaataca ttgttgttca tttgggatct cacatccacg ccattgtagg tcattgtagc
    29221 aacttcaaag cctgcatctg gtactaatgt aaagattttg gtggatcccg gggttacagt
    29281 cagtacagag ccgttgctca gtgctacatt gttctctttt attgtaccat tagcaccaag
    29341 ataaacagta atgctgtatg tttctgcagc cccggtttca ttgaataaga tgaacaatcc
    29401 ccagataggg tctgactgat aagtggcagc agtacctatc ggcacatgta ccgtacaggt
    29461 ttcaatattc acacccgtga aagtgctgga agtaatgaga ggtggtgttg tgcgtggcat
    29521 cgttattttc acaatactcg aacaaccctg aaaggcacca ttacttatgt ttactacatt
    29581 tttaccaaat gttagttctg taagttttga acaagcttcg aaagcataat aacctatttg
    29641 ggttattgat tgaggaaaga ttacttcacc tgttaaaccg gaacaatcat aaaatgcttg
    29701 atcaggtatt gtggtaagag tattgggtaa tgttaatttg cctgttaagc cgctacatat
    29761 ataaaatgca ccgtcaccga tgcttttgag ggagttggga agtcttagct ctccgttaaa
    29821 gccagagcag ctacgaaatg ctttatcacc aatgactgta acagaattgg gaataatcaa
    29881 atctccggat aagcttctac attctgaaaa tgcaaaatga ccaatagaaa ttaatcctgc
    29941 tggaagtggt agcactgatg tcaaattaga acatccgtaa aaagcataat ctccgatagt
    30001 ttctaacgaa aaaggcaatt taactaatct gagtagtttt ttttcctgaa aggcacttgc
    30061 aggcaatgaa ttccctgcaa gcatagcacc cgataaatcc agatcgatca atgatgtcag
    30121 actgtttttt aactgaataa tatcagtact attcagatta ccggttatag taagtgatgc
    30181 aatacccgaa agtggtatgt gcccggcagc gattaaagca gcagccaatc caccggatgt
    30241 cggtacatgg atagttattt tttccgtact tccaatgttg aaagcattcc accctgttgc
    30301 tgctttatag gcatttacgg aagatgcaga cacaagaatt acaactctac tattactact
    30361 actatcgaaa tcaaatgtat tcgtccccag ggttggcggt gttggattta aactggtaat
    30421 ggtattcaga ctagagcatt gatagaatac tgcatcttca atcgtgctca ctgaactacc
    30481 taaagtaact tcagtaagtc cagaacaatt ataaaaagcc tgactctcta ttgatgttac
    30541 catatttgga attacaatac ttgtcaatcc tttacatttg taaaatgaat tagttcgaat
    30601 tgatgttaaa gtagttggaa gctgaattga ttttaaactt gttttaccaa tagttttacc
    30661 aaaagtacta aaagaatgca cagggatttc atttgccgga taagttgtat aagaaaatgt
    30721 tccatcacta ccaaaatatt ccatgatgtt aacagcactc atgtctatat tagcaagtat
    30781 cgtcatttca tcacgcatac actttacatc acgtacatcg atattcccgg tcagggtaag
    30841 gtcggtcaca gtggttttct catccgcggt taacagggtg gtgagtgtac cgggtgtagt
    30901 tacatcgatt gttttagtaa cttgtgctgt aagtatatta gccagcaatg caaacagcat
    30961 aatagtaaac aaatttttca taaatatttt tatttgaaag ttaattaatt cttggttatt
    31021 ttcattttcg ctatgcaaaa aacgcgaagt cctcgttccc aagcgataat cgcgcaggaa
    31081 cgaggactta tatatgcatt aatttgctga tggagcttct tcgctggcgg gggctttgcg
    31141 tgagccggag ttcacaatgc ggcgggcatt gaggtatgcg gcctcaaatt cggtgccgtc
    31201 aaagaggttt atcaggctgt ctaatttgta aagcagtttg cggcttgcct gaatgagttc
    31261 gggcaggtct tcgttatgc
//
LOCUS       DAOPYB010000018        30489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1455_length_30489_cov_9.977755, whole genome shotgun sequence.
ACCESSION   DAOPYB010000018 DAOPYB010000000
VERSION     DAOPYB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 30489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30489
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1455_length_30489_cov_9.977755"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..513
                     /locus_tag="PKH58_02745"
     CDS             247..513
                     /locus_tag="PKH58_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNX87976.1"
                     /translation="MLESEENIKEKVRQHIFKATLSDGEIIKDDSPIFELGLLNSMGL
                     LYLIDFIKKEFDADVTDNELTKENFENINNITSFVNNKLQKKNE"
     gene            656..>1126
                     /locus_tag="PKH58_02750"
     CDS             656..>1126
                     /locus_tag="PKH58_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNX87977.1"
                     /translation="MCGIAGFYSNNVDENAPVILKRMLTRIKYRGPDESGIYISKKAC
                     LGSVRLSIIDPKTGCMPLSNEDKSLLIVYNGEIFNYIELKGELLKKGHHFNTHSDTEV
                     ILHLYEEYGPECLKKLNGQFAIAIWDTRKKELFLARDRMGIRPLFYTEKNGSFIF"
     assembly_gap    1127..1226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1227..2416
                     /locus_tag="PKH58_02755"
     CDS             <1227..2416
                     /locus_tag="PKH58_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNX87978.1"
                     /translation="SGGLDSCITTSFIKEVAPDNLQTYSIGFTDKEYDESIYQDMANE
                     FLKTYHNRIICSNNDIAEKFADLIWHSETVLLRSAPAPMYLLSGLVKSQNIKVVVTGE
                     GADELLGGYNIFKETIIRQFWSREPSSELRPLLLKKLYPYLPQIKSANSAAIKLFFGY
                     KLEENNSPVYSHLLRWNNTSHIKNYFSSETTKEISGYNPVNELIDNIGHKFDDIDLLS
                     RAQWLEMKLFMSGYLLSSQGDRMSMANSVEGRYPFLDYRIVDFCMKLPPDYKLHGLKE
                     KYLLKKMMAGKLPPQILERPKQAYRAPIPACFTSDSAPGFISEMLSEKQLISVGIFNP
                     DKVLYLITRLKNNNQVSETDNMALMGILSTQLLDYLFVKRNIPILNEKELLKCNVEIY
                     DDY"
     gene            2440..3420
                     /gene="nadE"
                     /locus_tag="PKH58_02760"
     CDS             2440..3420
                     /gene="nadE"
                     /locus_tag="PKH58_02760"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNX87979.1"
                     /translation="METDKKPFSSDILRHDNIESVCNNLVLQLKENVLLQLNRRGGVV
                     GISGGIDSSVTFALSVIAFGAENVLGIMMPEADSNPQSEDLARKLAEKLGVKTITENI
                     TPVLEKLECYKRRDEAISNVLPEFNPLTDKAKIVLKQKIKNNIPPAFSVTIIKPDGTE
                     ISKLLPVKDYLQIVAASNFKQRCRMSMLYYHAERLHYAVIGTPNKHETEQGFFVKYGD
                     GGADVMPIGGLYKTQVYQLAEYLDIPQEIIDRVPTTDTYSAEQTQEEFFYQLPFKTMD
                     LIWYGWENKYTPAEVAKELGKTEDDIENIYKNFERKRKTADYQRRPPIFF"
     gene            3523..4986
                     /locus_tag="PKH58_02765"
     CDS             3523..4986
                     /locus_tag="PKH58_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX87980.1"
                     /translation="MNAFDYFFSDTKYLEKEFILGVSEKATFMQIYNDSLKLACHLQK
                     IYGTSNNFIILSQNSVFFITVYLGIIKSGNVCIPLNSLIEQENLNYIIGLTGCKLIFL
                     SRNIRSGLQISEKIFDESTLETIVKQTETDSVYFDQAKFDDKSIAEIIFTSGSTGKPK
                     GVMISHLNLCTNTNSILKYLKLTSADIIEVVLPFYYCYGLSLLHTHLKVGGSMVLNNS
                     FILLGSVIDNLIKYRCTGFAGVPSHFQILLRKSQTFTKTQFPDLRYVTQAGGKLHSVF
                     IEEFARYFPEINFFVMYGQTEATARLSYLPPELLKSKIGSIGKAIPDVILKVVNEKNE
                     QVEVGEVGEIVARGDNVMAGYYHDPEGTAEVLKNGWLYTGDMARTDNDGFIYLVSRKK
                     EIIKVGGNRISPKEIEEVILSVPEILDCTVAGVDDELLGEAIKATIVIASSVNKNMIE
                     NKILEKCREKLALYKIPQQFEFTDKMNISSTGKKVKN"
     gene            5015..5797
                     /locus_tag="PKH58_02770"
     CDS             5015..5797
                     /locus_tag="PKH58_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNX87981.1"
                     /translation="MRFDGNYTISNSAKIGKNVKIGRNTIIHDNVEIGDNSIICENCI
                     IGEPVFASYYDSNYVNPKTVIGANSLIRSGSIIYADSIFGENFKTGNIVSIREKSVFG
                     KNCIVGTQSDVQGNVTFGDYCRLNSHVQIGCKCTFGNFIFIYPYVVFTNDPHPPSKEL
                     IGSTVCDYAQIAAGSVILPGVVIGKHSLIGANSLINKNVGDYELHAGNPAVFVCKVNY
                     IWSNENKRPHYPWPYSFDKELPWEGKNFDDWLNTEEGMKYAK"
     gene            6041..6838
                     /locus_tag="PKH58_02775"
     CDS             6041..6838
                     /locus_tag="PKH58_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNX87982.1"
                     /translation="MLQSYTIYNKSLNEISQSKAIINTLNAHSFNTVHTDKDFQKALQ
                     SSDVLLPDGISVVLAIRMLTGQKIKKIAGEDLFHYEMNRINNAGGKCFFLGSTEKTLQ
                     LIQKRAASEYPGIRISGYSPPYKPEFSEDDTRAMIEAVNKHEPDVLFVGMTAPKQEKW
                     VHNNSHNLKAGHVCCIGAVFDFYAGTVKRAPAWMIKIGMEWFYRLIREPRRMWRRYLI
                     GNIKFIGYILREKLLISMKKNKVQHADVQSIKGEKEVFIEDINEMLP"
     gene            6915..8303
                     /locus_tag="PKH58_02780"
     CDS             6915..8303
                     /locus_tag="PKH58_02780"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNX87983.1"
                     /translation="MDTDEVGMKFVYAIVDLILLNAAIAIVYSMSPVFAEISLPDRNL
                     IYLHGNISELIAYSLYSKRNYYFRDRYANRVKALNYRMAIFVLTLFILAQIFLHKPIS
                     YLFLLKYSAFFYVLKLLVLYLLYRFFRYRRKKGYSIHRVLIFGLNKTGVILGDLIKNN
                     PELGLQLVGFITEEHDYKKCEKKVLGKYEDLARLTEELNIDMLYVTIPKYYETNLTRE
                     LLAICNKIGIRVRYVPVNQSWFKARVKNTEYVGSFMMYNPQEIPLDRLTYRLAKRAFD
                     IIFSLLIIILLFSWLFPILALIIKLDSKGPVFFLQRRTGINNKTFYCVKFRTMRTNQD
                     ADTRQATKNDDRITPIGKFLRKTNIDELPQFLNVLIGDMSVVGPRPHMLKHTKQYSAL
                     IEHYKARHFVKPGITGWAQINGYRGLTDELWKMEKRVQFDMEYLEGWTFIWDLRIVLL
                     TIFSKGVYKNAG"
     gene            8443..8643
                     /locus_tag="PKH58_02785"
     CDS             8443..8643
                     /locus_tag="PKH58_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNX87984.1"
                     /translation="MLVNILLFSLGAPEVILIALVVLLIFGGKKIPELMRGLGKGVSS
                     FKKGLKDMDDEIKNDLKDDSEK"
     gene            8686..9513
                     /gene="tatC"
                     /locus_tag="PKH58_02790"
     CDS             8686..9513
                     /gene="tatC"
                     /locus_tag="PKH58_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNX87985.1"
                     /translation="MGKPDSEKEFTFWEHLEALRWTILRSLGVVTVLTFVFFGFKDFV
                     FSKIILPPLNSDFYTYRLLCLLSEKLKMPGLCPDDFSVQLININLAGQFITHITSSLI
                     MAVVLSMPYLLFEIWRFINPALYEHERKNISIIFLSSSFLFYLGALVSYFLIFPLTIR
                     FLGTYQVSGLVTNQISIQSYMNALGILIFSLGIMFELPIVIYALNRIGLVSKKALKSF
                     RKYAFVGILIIAAVITPTTDPFTMLVVALPIYLLYEVSILLCNKDLPALESDSEEED"
     gene            9690..11021
                     /locus_tag="PKH58_02795"
     CDS             9690..11021
                     /locus_tag="PKH58_02795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX87986.1"
                     /translation="MQFSDFNLNNPLINALNDLGFTEATTIQEKAFPVIMSGRDMVGI
                     AQTGTGKTIAYLLPCLRQWKFTVEKHPQILIVVPTRELVVQVTEEIKKMTTYMNIRVA
                     GVYGGENMIRQAPLILEGLDILVATPGRLLDFALNGTLKLRLVKRFVIDEVDEMLNLG
                     FRPQLIRIMDLLPRKRQNLMFSATMTTEIKDFIADFFIDPQEIEAARTGTPLANIKQI
                     GIKVPNYNTKINLLEFLLKTEPGFSKVLLFVSTKKLADDVYSRISTLFPETIGVIHSN
                     KTQNNRFETLNSFNKNALRVLIATDIVARGLDFDDITHVVNFDVPEEPENYMHRIGRT
                     GRVDKKGVAVTFITKDDTTKRENIQLLMGQKIPMMRMPEEVKISTELTESELPQIITG
                     NSVAFVSRKEDHGPAFHEKKDKNKKVNMKIRRKEALKLKYGKPKTRGQKKK"
     gene            complement(11035..12426)
                     /locus_tag="PKH58_02800"
     CDS             complement(11035..12426)
                     /locus_tag="PKH58_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNX87987.1"
                     /translation="MNNKSLLLIALFVALIFSSCKTLKTERPKESYLPSDLLPAYSEL
                     PLHLELDIKQLETSVNNNLNGLLYEGSNITDKDLSIKVWKSGDFTFTVNNNNIEYRIP
                     LKIWSRFAWKVQKLGVTLADDYEATGIIALKYKTSVGIDKNWNLVAETASQGYEWIQT
                     PKLNMIGVNVPVKPIADLALSKYGKTITQQIDKSLGEFANLNKQVAAAWTELQKPVQA
                     SAENDVWVRIIPKDILVSPFVTKGGKLQTDISLYGRVETIMGSKPAAGKPVALPPFKR
                     SNSAPQQFNLNVASDVTFDKISELAKQQLLNQTFSQGKKYIKITDLSVFGSEGKAVFS
                     ADVIGSVKGRIYFTGDMIYNPSKMTVEVVNPEFELKTKNTLIKSADWLLHGLILKKIV
                     PYLSFPVKEYTDQMKTEANVMLKNYKVYEGVSLQGKLDDFTMKSVSLVPGAVRIQANL
                     KGNVMLKLANIKF"
     gene            complement(12463..12915)
                     /locus_tag="PKH58_02805"
     CDS             complement(12463..12915)
                     /locus_tag="PKH58_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNX87988.1"
                     /translation="MKYNRLIISIILMSVFCVAFPQRYDARLAKKLGADEYGMKNYIL
                     VILKTGPNNTTDKQDLDTLFRGHMNNIQRLSEEGKLIVAGPLGKNPNSYRGIFILDVK
                     SKEEALKLLQSDPTVREKVFEVELYDWYGSAALPVYLKTHTRIEKKKH"
     gene            complement(12932..13861)
                     /locus_tag="PKH58_02810"
     CDS             complement(12932..13861)
                     /locus_tag="PKH58_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87989.1"
                     /translation="MKKITVFLLFVFCGFSLFGQINAADSTVQVVGYWNVHDKQTYQV
                     TEESNKIQNATDTISKQKIAYLLDVEVLDSTANSYTIQWLSHDVKVLNTPNPLLDDLY
                     KIGENSKIIFRVSETGVFKEILNWQEMQAQNQKAFEMLRTKFDTIPQLVNMINGLESK
                     LNSKEAIEGSSVKLINQFYSFHGGKYKLGEEVNAPVKLPNAYGGEPFDGMLTVLLDEI
                     DTDNDYSIIRSWQTADSGQLTEFAKKIVKDIASDMNLDIQNEQLDFPPMVNETRIASQ
                     IHGNTGWVIYSNMTTEVSLNNVLNIEETTIELQ"
     gene            complement(14081..14617)
                     /locus_tag="PKH58_02815"
     CDS             complement(14081..14617)
                     /locus_tag="PKH58_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX87990.1"
                     /translation="MRKLFLFFALFMAILPSCKEVDKLTQFDLPVNQQFTLPGSLPVM
                     NGFSFSTPDVETNYMSYFEMFNINTELVESICIKSATFKINKPQTADFSFLKSVEVYI
                     SADGLEPVLMASKKDVNTNVGNSLNLDVDSKVNLKDYVTKNNIKLNVKVTTVRATTEE
                     YVVDTKLVFGVDAKVIGL"
     gene            complement(14822..15238)
                     /gene="mscL"
                     /locus_tag="PKH58_02820"
     CDS             complement(14822..15238)
                     /gene="mscL"
                     /locus_tag="PKH58_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNX87991.1"
                     /translation="MGFIKEFKEFAIKGNVIDLAVAVIIGGAFGKIIASFVADIVMPL
                     IGLLLGGVNFTELKWVIRPAEVVNGVEKAAATLNYGNFIQVTIDFLIIALAIFLAIKG
                     MNSFKKKEEPAPEAPAAPSKEEVLLAEIRDLLKEKK"
     gene            complement(15479..17131)
                     /locus_tag="PKH58_02825"
     CDS             complement(15479..17131)
                     /locus_tag="PKH58_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNX87992.1"
                     /translation="MKTKFLYILTTLSVISFFYSCKEPPVLLSNAYIELLSVNGKSVA
                     SDGIVMNVPVDSVIIRVIFSTEIDNSKIRPDKIYFTNNEEVQFDILTTENPKELKIKL
                     KDKLNYYTKYTLNINSGEYMGVNIIDNSKVNFLTELDTTPKFPTISSDSLLTLVQKQT
                     FRYFWDYAHPVSGLARERLGSGETVTTGGSGFGLMAILVGIERGFISRSQGYAQIDKI
                     VRFLNRPETDKFHGAFPHWLNGTTGKVIPFSTKDNGADLIETSFLMQGLLAVQAYFKS
                     GNPPEQAMCAIIQQLWEKVEWNWFQKNGEQKLYWHWSPNYNWDMNMPITGWNEGLIAY
                     VLAASSPTYPIPKRVYDEGWARNGAMKNGNSYYGVTLPLGEAYGGPLFFAHYSFLGLD
                     PRNLSDTYASYWIQNKAHTSINYKYCVANPKGYYGYDENCWGLTASDFPDGYTASSPT
                     NDQGTITPSAALASFPYTPVESTKALNFFYYTLGNRVWGEYGFKDAFSLSRRWFAESY
                     LAIDQGPIIVMIENYRTGLIWNNFMSCPDVKAGLTKLGFIYR"
     gene            complement(17209..18090)
                     /locus_tag="PKH58_02830"
     CDS             complement(17209..18090)
                     /locus_tag="PKH58_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNX87993.1"
                     /translation="MKTLKIFGLIAFATFAFTFTGCKKDEPKLPPIGGYDNADEVGKA
                     DLVAYWPLNGDGNESLSSSAPAKTSNVTWVDGIKGKAASFNVGYLAYNTIANLGTNLN
                     TGFTVSSWVKLTNNGANSSVIFSLSRPNEWAGSINFMSETGWMPATSDSLTVKGLIVS
                     STDLGWQDSRNTVKASAADIAAGHIAYPNKIGGKWAHVVLTWNNTTKLLKVYVNGIKI
                     SNPVWENRDNTGTKNFVVTTPCHPILGAFETFANGTTTDAWNTGLKGQLDEVRVWKRE
                     LVLADIGALYELEKAGR"
     gene            complement(18154..19608)
                     /locus_tag="PKH58_02835"
     CDS             complement(18154..19608)
                     /locus_tag="PKH58_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX87994.1"
                     /translation="MKHVKILSIITFCLTLFLVGCEKDLNVLPQDKMSKDDFLAEPLY
                     AVEVVNSAYNQMLSFEMYGFPWIGVTSITSDDADKGSSDGDAGTDKDKLDDLAFDKSL
                     RSFNDVWKRRYEGIYRCNEGIYYLEKVNLEENIKNRYIGELKFLRAMFYFDLVRCFGG
                     VPVVVEKLDFKEEATVNQVVYSRKTKAETYAQIEKDLMDAAASLPLQYNRENLGRVTK
                     GAAQGLLAKVYMYEEKWNEAFAMAGNVVTSKMYDLLPDYANVWREVGENSKESLFEIQ
                     ATLGKGVNGYTDVQSGRGTPDHGWGFNTPTVNLANAYEPGDKRKDGTIMFTGQVLWDG
                     FQTPATGWANPRYNYKAYQSRTKESWDDNKGETAKNVRLLKYSDVLLIRAEAGYHINQ
                     LDTALSIVNKIRKRAGLIPLTSVTLDQIYKERRLEMAMEHDRWFDIVRTGKASVAMSA
                     VGKVFIQGKHELFPIPEEQITLSKGILTQNPNYD"
     gene            complement(19653..22637)
                     /locus_tag="PKH58_02840"
     CDS             complement(19653..22637)
                     /locus_tag="PKH58_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX87995.1"
                     /translation="MKIKRTTIFLLMFISVFSIVAQSVTISGVISDKKTGETLIGAKV
                     LQQGTNNGTITNFDGNFSISVPLNSILEISYIGYKSQEVSVRSNAPLNIQLETNDKVL
                     DEVVVVGYGTKKAGAITGSVVQIKSDDIMKTPAQSAIQSIQGKAAGVNIIATDEPGRA
                     PTVMIRGINTVLGGGTPLYVIDGTEANSLNGLSANDIETIDVLKDASSLAIYGNKAAS
                     GVILVTTKKGKKGSVKVIYDGYVGFKTMMKTVDMADAYHFAYYQNFAAGRTRFASTQP
                     YDTNWLKEITQTGMTTNHSISLSGGEENVNYYFGISRFDEQGILKGTDYDRTNILSKN
                     EYRLFNRLKISQSINVAVVNNVPQPSSAFTAAYKQSPIMPVKYENGKWGMPFLDNATG
                     EIGMIGDKFNNVPNPLAMLGYNEDKNRNVTLFGTVGAELQIYKDLKFNSNFGATFDYG
                     MGYSYTPNKENWFSQNPTATEDDFYKNNKFYSTLSQSRSHFFVWNWDNFLTYSKDFDK
                     HGITAVLGVTRSTTRTAQYLSGRRNNVPYQPNYWSLDLSKSNIVTSPDNVVSNSSQTP
                     LVNLGYFARVDYDYERKYLLTASLRRDGISDFQSAKKWGIFPAVSGGWVVSSEDFFEK
                     YASVIDYLKIKAGYGEIGNGNAAPSVNQILFQNGATYPFGSPEIIYPGSFVPYAVDPD
                     LTWETTKEFGGGIEFRMLKQRLYGTLEFYRKLTDNVILPVKLPTVLSPGDVYLNAGTV
                     ENKGIEATFSWNDKLGDEFNYRAGVNFAVNNNAIKQIYSSYFDKLTGGDLGNGHYTKE
                     IHIGDPIGSFYVYEHTGYTEDGAFAYSSERINAGSYIPKLTYGFNLGFDYKNFDFSTD
                     WYGVMGNKVYNGKKAQRWGGENVEMNEFNDFWIPSNPKQDAANPRPYSDTPLASTYFI
                     EKGDYLRINNVTIGYSLPKVLKEIQKLRVYVTAVNPFIFTKYTGFTPELIGSGTPLGS
                     AGIELDAYPTNRSLTFGLNITL"
     gene            complement(22643..24232)
                     /locus_tag="PKH58_02845"
     CDS             complement(22643..24232)
                     /locus_tag="PKH58_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNX87996.1"
                     /translation="MKKLKSIGLLLMIFGVLSAQSQYVVPNELKAQSFPGHSEICWKN
                     HQGFTYEVYRLVGNAKSFVKQGETTQDNFLDFYERNIPPNTEIRYRIIPKGMKVTDSD
                     AAKFEIKVTRKAYRDEDLLDMTQRYTTRYFLDFAEPGTGMARERSNDVNGDIVTTGGT
                     GFGLMAIIAGIERNYFPKEKGFEVINKIVSFLGSVERFHGAWAHWYNADTRKAFSFSK
                     YDDGGDLVETALLMQGLLTAREYLKKGNDKEHELANKITHLWEAVEWNWYTQGSDSLY
                     WHWSKNYGWKMNHCIKGFDETLITYVLAASSPTYPIDAKVYEACFKNSPYYYNGKSYY
                     GIKLDLGMDYGGPLFFTHYSFLGLNPNGLTDSYTNYFERNKAHTLIHRAYAINNPKKH
                     TGYGANLWGFTSSDDPIVGYSSHHPGTDSDNGTVPPTAALSSIVYTPKESMEVLKYLY
                     FEKGKQVFGKYGFYDSYNPGLIEGQQVVKSYLAIDQGPIAVMIENFRSGLVWNLFMGN
                     REIQNGLKKLGFNFQLTSNNK"
     gene            complement(24236..26581)
                     /gene="bglX"
                     /locus_tag="PKH58_02850"
     CDS             complement(24236..26581)
                     /gene="bglX"
                     /locus_tag="PKH58_02850"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNX87997.1"
                     /translation="MMLIGLTPVNSAYAQKKIKKTPVDIVSKPVQTKVVSTNSTHFKP
                     NPAMDQFIGDLMSKMTLDEKLGQLNLPSADDFTTGQAKSSNIGQKIKEGRVGGLFNIK
                     GVDKIREMQRVAIEESRLKIPLIFGMDVIHGYETTFPIPLGLASSWDMELIKRSAQIA
                     AQEATADGINWTFSPMVDIARDPRWGRVAEGSGEDAYLGSQIAKAMVYGYQGNDLSAS
                     NTMLACVKHFALYGAAEAGRDYNTVDMSRVRMYNEYLTPYKAAVDAGAGSVMVSFNEI
                     DGIPATGNKWLLTDLLRNQWQFPGFVVTDYTGINEMIAHGMGDLQQVSKLALNAGVDM
                     DMVGEGFLTTLTKSLKEGQVSYSAIDAAVRRILEAKYRLGLFEDPYKYCDANRAKTEI
                     FSNQHRTEARSIAAQSMVLMKNENNVLPLKASGTVAVIGPLADNPENMPGTWSVAAKH
                     AKAVSMLKGLKETLGDKVNFVTAKGANFDYDAQFESRISMFGKNSYRDNRPKEEILKE
                     AVEVARKADVIVAALGETAEMSGESSSRSNIEVPQSQKDLLVELKKLGKPIVLVLFTG
                     RPLVLNDESKLADAILNVWFAGSEAGYAISDVIFGKVNPSGKLPMTFPRNVGQVPIYY
                     NHKSTGRPENKEDQFDKFRSVYLDVPNSPLFPFGYGLSYTTFSYGDINLSNTQLQGNQ
                     TLKASISVTNTGKYDGAEVVQLFIRDVVGSNTRPVKELKGFRKVMLKAGESKTVSFDI
                     TPEELKYYNNDLKYDWESGDFEIMIGTNSHDVKSVTINWQK"
     gene            complement(26838..29702)
                     /locus_tag="PKH58_02855"
     CDS             complement(26838..29702)
                     /locus_tag="PKH58_02855"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNX87998.1"
                     /translation="MWKIRILITSFLVAIQIFIFAQEFTPIVNHYSKKDYNASNQNWD
                     VKQAADGKMCFGNNQGLLQFDGSNWKVYKIPGNKIIRSLFISSDKKIYVGSFEEFGYF
                     EKDNKGLLVYTSISDKLGNYNMQNDEIWNILETDGKIIFQSFTSFFIYDKHNLRGKRF
                     PLTFLFFQKFKNEIYTHTDQKGLCYYDKKKEDFIPVNSAPFKSAVVAMLPINHYEAFI
                     VTKADGIYIFNGIDFRRLITDTDRELSRANINKAVLTKDSLLVIGTIQNGVTALNYKG
                     QKVWSLNTNNVLQNNTVLGMCCDQENNLWLALDKGISMLRLGKSLNYIHTFSPAIGAI
                     YSVNFQEPDNLFIATNQGFYRAKLNITAKRIENVTLDPRITGQVWNISRFDNQFLCGN
                     NEQTFEVDKTQSKIVSPVKGGICISKGIIHGKEVLVQGTYTELCIYEKKNNVWIFSHS
                     VSNFLNPIQYIEVDYKGTIWAAHLHAGLYAIRLKEDLRTIESLKVYKSLDNVHKVPIN
                     VFSINNRIVFTDNMAFYTYDDIQKKIIPYDLINNSLGRFSQAYRVCSSSPSYYWFIRN
                     DEAALVKIQNDKINIIDQIQNSLFLNQTVDNYQNIFYLKDKMSLITLENGLALYERQN
                     GKMNLSKYKLQISKVEVSDRKNRKIINFPVVADSEVKISYKNNNIRFSVFYPVYSHLN
                     NLSFRYRLIGLDDVWSRSTPENSKEYSYLSPGKYTFEVQALSNSNDVLSTVSYDFLVR
                     PPFYWNTFSKILYILLLTLMVYFAYWLVKRNFRIKKQKIHEEQEAIRKKEIEKREQQI
                     IALKNEKLESEIVQKSKELAGSTMTIIKKNELMNTIKDEIVSLKNKLGTQYPNKYYDK
                     LIKLLDENISSEDDWAIFQANFDRIHENFFRNLHVTYSELTANDLRFCAFFRLNLSTK
                     DIAHLMNISLKGVEVARYRIRKKMNIPSEKNISEFLIEFK"
     gene            complement(29891..>30489)
                     /gene="secF"
                     /locus_tag="PKH58_02860"
     CDS             complement(29891..>30489)
                     /gene="secF"
                     /locus_tag="PKH58_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNX87999.1"
                     /translation="NIVNSQKVGPTIAEDIKTSAIWAVILSLLGVSLYIFIRFNHFAF
                     SMGTMLCLAHDTIIIIGSFALLWGIMPFSMEMDLNFIAAILTYIGFSVNDTVVIFDRI
                     RENTGLYPKRDKKDVINEALNETLSRTFSTSASVLVVLFAIFIFGGDTIRGFIFALLI
                     GSILGVYSTLFVAVPVAYDIMLSQKKKKELKAAAELKK"
BASE COUNT         9282 a         6057 c         5466 g         9584 t
ORIGIN      
        1 aattgcacat cttcactgag atactctttt acctggggac ctgaaagagt ataaaaaata
       61 acccgatcga ataccttttt ccactttcag gtgtcattaa tatagtaatt aaaaaaacta
      121 tttaaaactt aattgtattt tccaaacctt aaaacaattt tgacttattg ttgtttaaat
      181 aaaacatcac ataaagcaaa tatcattgat aatatgactg ttacaatcaa ataaaaactc
      241 atcatcatgt tggaatccga agaaaatata aaagaaaaag taagacagca tattttcaaa
      301 gctactctgt ctgatgggga aattatcaaa gacgattccc cgatatttga attgggcctt
      361 ctcaattcta tgggattact ttatcttatc gattttatca aaaaagagtt cgatgcggat
      421 gtaacggaca acgaactaac taaagaaaac tttgaaaaca taaataatat aacatcattt
      481 gtaaataata aattacagaa gaaaaatgaa tgatcgttgt tataaaatat atcaggttaa
      541 attgatggaa aaatcattga ttatcaacca acttaagtct ggagttttga tgtgttaact
      601 tcatacaact ttctcaagac actgttatct gttttgttta ttaatctttc ggaaaatgtg
      661 tggtattgct ggcttttact caaataatgt ggacgaaaat gctccggtca ttctcaaacg
      721 catgttaacc cggattaaat acagaggtcc tgatgaaagt ggtatatata tttcaaaaaa
      781 agcctgtctg ggcagtgtca gactaagcat cattgatccc aaaacaggat gtatgccact
      841 atcaaacgaa gataaatcgc tgttgatcgt atataacgga gaaatattca attatattga
      901 attgaaaggt gagctactga aaaaaggtca tcattttaat acccactcag ataccgaagt
      961 aatacttcat ctctacgaag aatatggccc ggaatgtctc aaaaaactca acggacaatt
     1021 tgccattgca atatgggata caagaaaaaa agaattgttt ctggcccgcg acaggatggg
     1081 aatcagacct cttttttaca ccgaaaaaaa tggcagtttt attttcnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnntgag cggaggtctt gattcgtgca ttactacttc
     1261 atttatcaaa gaagttgcac ccgacaacct acaaacgtat tctattggat tcaccgataa
     1321 agaatatgat gaaagcatat atcaggacat ggcaaatgaa tttctcaaaa cgtatcacaa
     1381 ccggattatt tgcagcaata atgatatagc tgaaaaattt gcagacttga tctggcattc
     1441 cgaaactgta ttactgagaa gcgctcctgc acctatgtac ctgttgtccg gattggtaaa
     1501 aagtcaaaat ataaaagtgg ttgtaaccgg tgagggtgcc gatgaacttt taggaggtta
     1561 taatattttc aaggaaacaa taatcagaca gttttggtcc agagaaccat cttcagaact
     1621 tagacctttg ctgttaaaaa agttgtatcc ttatctgcct caaattaaat cggcaaacag
     1681 cgctgccata aaattattct tcggatataa acttgaagaa aacaattctc cggtttattc
     1741 acatttactc cgctggaata atacatcgca tattaaaaat tatttttcca gcgaaactac
     1801 aaaagaaatt tccggctaca atcctgtaaa tgaattgatt gataacattg gtcataaatt
     1861 tgatgatatt gatcttttat cgcgtgctca gtggcttgaa atgaaacttt ttatgtcagg
     1921 ttatttactc tcatcgcaag gtgaccgtat gtcaatggca aactcggtgg aaggaaggta
     1981 tccgtttttg gactatcgaa ttgttgattt ttgcatgaaa ctgccaccgg attacaagct
     2041 gcatggactc aaagagaagt accttctgaa aaaaatgatg gcaggaaaac ttccaccaca
     2101 gatattggaa aggcctaaac aggcatatcg ggctccaatt ccagcttgct ttacgtcgga
     2161 ttctgctccg ggttttatca gcgaaatgct gagtgaaaaa cagcttattt cggttgggat
     2221 ttttaatcct gataaggtac tgtatttaat aaccaggctt aaaaacaaca atcaggtttc
     2281 ggaaactgac aatatggctt taatgggaat tctgtcaaca cagcttcttg attatctgtt
     2341 cgttaaaaga aatatcccaa tactgaacga aaaagaattg ctaaaatgta acgttgagat
     2401 ttatgatgat tattaaaata aaacttcaaa aatgcgtaca tggaaaccga caaaaaacct
     2461 ttttcttctg atatcttacg gcatgataac atcgagtccg tttgtaacaa tttagtcctt
     2521 cagcttaagg aaaatgttct gctacagctt aatcgccggg gaggtgtagt aggcattagc
     2581 ggaggaattg attcttcggt tacattcgca ctttccgtca tagcatttgg cgccgaaaat
     2641 gtattaggta taatgatgcc tgaagcagat tcaaacccgc aaagtgaaga tctggcgcga
     2701 aagctggctg aaaagctggg agtaaaaacc ataacggaaa atattactcc cgtactcgaa
     2761 aaattagagt gttacaagcg acgcgacgaa gcaatatcaa atgtgttacc tgaatttaat
     2821 cctttaaccg acaaagcaaa aattgtatta aagcaaaaaa taaaaaataa tattcctccc
     2881 gctttttctg ttactataat caaacccgac ggaactgaaa tcagcaaact tttacctgta
     2941 aaagattatt tgcaaatagt agctgcttcc aacttcaagc aacgatgcag gatgtcaatg
     3001 ctttactatc atgcggaacg actgcattat gctgtcattg gtactcccaa taaacatgaa
     3061 acagaacagg gattttttgt aaaatacggt gacggtggtg cagatgttat gccgattggt
     3121 ggtctttaca aaactcaggt ttaccaatta gctgaatatc ttgatatccc acaggaaatc
     3181 attgacaggg taccaactac agacacttac agtgccgagc aaacacagga agagtttttc
     3241 taccaattgc ctttcaaaac aatggatctc atttggtatg gctgggagaa taaatacaca
     3301 cctgcagaag tggcaaagga actcggaaaa acagaggacg atattgaaaa tatatacaaa
     3361 aacttcgaaa gaaaaaggaa aacagctgat tatcagcgaa gaccaccgat atttttttaa
     3421 tcgataaatt tcgaacagac attgatgaag tattgtttaa ataaaaaaac ttataaaatt
     3481 gccattggat taagttgaga caaatcttta taaaatgtgg ccatgaatgc atttgattac
     3541 ttcttcagcg ataccaaata cttagaaaag gaatttatcc taggagtatc tgaaaaggct
     3601 acttttatgc aaatttataa cgacagtctg aaactggcat gtcatctgca aaaaatctac
     3661 ggaaccagca acaactttat aattctcagt cagaattcag ttttttttat aacagtttat
     3721 cttggaatca tcaaatccgg caatgtctgt attccgctca attctctcat tgaacaggaa
     3781 aacctaaact atattatcgg acttaccggt tgcaaactga tttttttatc ccgaaacatc
     3841 aggtcaggtt tacaaatctc cgaaaaaata tttgatgaat caacccttga gacaatcgta
     3901 aagcaaacgg aaactgattc ggtgtatttt gatcaggcca aattcgacga taaaagtatt
     3961 gctgaaataa ttttcacatc tggctctacc gggaaaccaa aaggtgtaat gattagtcac
     4021 ctgaatcttt gtacgaatac aaattcaata ctcaaatatc ttaaacttac ttcagcagat
     4081 atcatagaag tagtattacc attttattac tgttatggat tatcgctact gcatactcat
     4141 ttgaaagttg gtggttccat ggttttaaac aactcattta tacttttagg atcagtaatc
     4201 gataatctca tcaaatatcg atgcaccgga tttgcgggtg taccaagcca tttccagata
     4261 cttcttcgaa aatcacaaac ctttacaaaa acacaatttc ccgatctcag gtatgtaact
     4321 caggcaggag gaaaactgca ttcggtattt attgaagaat ttgcccgata ttttcctgaa
     4381 atcaatttct ttgtaatgta cgggcagacc gaagccacag cgagactttc ctatttgccg
     4441 cctgaacttt taaagtcaaa aatcggctcc atcggtaaag ctattccgga tgttatcctg
     4501 aaagttgtca atgagaaaaa tgagcaggtg gaagttggag aagtcggtga aattgttgcg
     4561 cggggtgata atgtaatggc tggttactac cacgatcctg aaggtacagc tgaagtacta
     4621 aagaatggct ggctttatac aggtgatatg gccagaacag ataatgacgg atttatatac
     4681 ctggtgtcaa gaaaaaaaga aattatcaaa gttggaggga acaggataag tccaaaagaa
     4741 attgaagaag ttatattgtc cgtacctgaa attctggatt gcacagtggc tggagtagat
     4801 gatgaattat tgggagaagc cataaaggcg acaattgtca ttgcaagttc cgtgaataag
     4861 aatatgatag agaataaaat tctggaaaaa tgcagagaaa aactggcttt atacaaaatc
     4921 ccgcaacagt ttgaatttac cgataaaatg aacataagct ctacgggaaa aaaagtaaaa
     4981 aattaactga aataatttaa taaaaaaatt caatatgaga ttcgatggca attacactat
     5041 cagcaactcg gccaaaatag gaaaaaacgt aaaaattggt agaaacacaa ttattcatga
     5101 taatgtagaa atcggggata attcaattat ctgcgaaaac tgtataattg gcgaaccggt
     5161 ttttgcatca tattatgaca gtaattacgt aaatccaaaa actgtaatag gcgctaattc
     5221 cctgataaga agcggctcca ttatctatgc agatagcatt ttcggtgaaa atttcaaaac
     5281 cggcaatatc gtttcaatca gagaaaaatc ggtttttggt aaaaattgta ttgtaggtac
     5341 tcaaagcgat gttcagggaa atgttacttt tggtgattac tgccgcctga atagccatgt
     5401 tcagattgga tgtaaatgca cttttggcaa tttcattttt atatatcctt atgtagtctt
     5461 taccaatgat cctcatccgc cttcaaaaga attgatcggt tcaaccgtgt gtgattatgc
     5521 acagattgca gccggatcag tgatattacc cggagttgtc attggaaaac acagcctgat
     5581 tggtgcaaat tcattaatta ataaaaatgt aggtgattat gaacttcatg ccggtaatcc
     5641 cgccgtcttc gtgtgtaaag taaattacat ctggtcaaat gaaaataagc gccctcatta
     5701 tccctggcca tacagttttg acaaggaact gccctgggaa ggaaaaaatt ttgatgactg
     5761 gcttaacacc gaggaaggga tgaaatatgc aaaataacag cgacaaatat atgttgttgt
     5821 tattttattc tagcaaatga ttttaaaatg caaatattaa cttataattg tttcatcaga
     5881 ttaaacttct tttacttgct tttgtttaaa atatacatcg aagattaaac agtgaattat
     5941 aatgattgat gggtaggaat gttttaatgt cgtttaagta aaaataaatt aattcatttt
     6001 taactttttc acaaccatta acaacctgac aattaagtct atgctgcaga gttacacgat
     6061 atataataag tcattaaacg aaatcagcca gtcaaaagcg attatcaaca cacttaatgc
     6121 ccattcgttt aatactgttc ataccgacaa ggattttcag aaagcccttc agagttccga
     6181 tgtgttatta ccggatggca ttagcgtggt actggctatt cgtatgctga ccggtcagaa
     6241 aataaaaaaa atagccggtg aagatttatt tcattatgag atgaaccgga taaataacgc
     6301 cggaggtaaa tgtttttttc ttggaagcac agaaaaaact cttcaactga tacagaaaag
     6361 agcagcaagc gaatatcccg gaattcgaat ctccggctat tcacctccct ataaaccgga
     6421 attcagtgaa gatgacacac gagctatgat cgaagccgta aacaaacatg agccagatgt
     6481 gttatttgtt ggtatgacag ctcccaaaca agaaaaatgg gtacataata atagccataa
     6541 tctaaaggct ggccatgtat gctgtatcgg tgctgtgttc gatttctatg caggcactgt
     6601 gaaaagggct ccggcatgga tgataaaaat aggtatggaa tggttttacc ggttgataag
     6661 ggaaccccgg cgtatgtggc gcagatatct gattgggaac attaaattta ttggatatat
     6721 tctcagggaa aaattattaa tttcaatgaa aaaaaataaa gtacaacatg cagatgtgca
     6781 atcaattaaa ggagaaaaag aagtatttat tgaagatata aacgaaatgt taccctgaca
     6841 tagtcaggaa tttttatacc tgatttgcat ttactaaacc tgaatattta tctgactttt
     6901 ttacaactac aattatggat acggatgaag tgggaatgaa attcgtatat gctattgtcg
     6961 acttaattct ccttaatgca gcaatagcaa tcgtgtattc aatgagccct gtttttgcag
     7021 aaataagctt accggataga aatctgatat atctacacgg taacatttcg gaattgattg
     7081 cctattcttt atattcaaag agaaattatt atttccggga caggtatgcc aacagggtta
     7141 aagcactgaa ctatagaatg gctatatttg tgctaacctt gtttatttta gctcagattt
     7201 ttttgcacaa accgatttca tacctgtttt tactgaaata ctctgcgttt ttttacgtgc
     7261 tcaagctatt agtactgtat ctgctgtata gatttttcag atatcgccgt aaaaaaggat
     7321 attctattca tcgcgtttta atttttggtc tgaacaaaac gggtgtaatt ctgggtgact
     7381 taataaaaaa caatcccgaa ctcgggcttc agctggttgg tttcatcact gaagaacatg
     7441 attataaaaa atgcgagaaa aaggttttag gcaaatacga ggatctagcc cgactcacgg
     7501 aggaattaaa cattgacatg ctctatgtca ccattccgaa atactacgaa acaaacctca
     7561 cacgtgaatt acttgccatc tgtaacaaga taggaattcg ggtaagatat gttccggtta
     7621 atcaaagctg gtttaaagca agagtaaaga acaccgaata tgtaggttca ttcatgatgt
     7681 acaatccaca ggaaatacct ctggatcgtt taacctaccg actcgcaaaa agggcatttg
     7741 acataatatt ctccttgtta ataatcatcc tccttttcag ctggctcttc cccattcttg
     7801 ccctgatcat caaacttgat tcgaaaggac cggtattttt ccttcaacga cgaacgggaa
     7861 tcaataataa aacattttat tgtgtcaaat tccgaaccat gcgtaccaat caggatgcag
     7921 ataccagaca agcaacaaaa aacgatgacc ggattacacc gatcggcaaa tttctccgaa
     7981 aaacaaacat tgacgaactg ccacagtttc taaatgtact gataggtgat atgtctgttg
     8041 taggaccacg tccgcatatg ctcaaacata ccaaacaata ctcagctctg attgaacatt
     8101 acaaagcccg tcattttgtg aagccgggaa taacaggctg ggctcaaatt aacggctacc
     8161 gtggactgac tgatgaactc tggaaaatgg aaaaaagagt acagtttgac atggaatatc
     8221 tcgaaggctg gacttttata tgggatttaa gaatcgtttt attaactatt ttcagtaagg
     8281 gcgtttataa gaatgctgga taaaatcaat catccggcta tgaaaaaatc tttaaaaagg
     8341 caaattgtgt tcgacagtct gcttttttta ttttcatatc tatgttatta aaatttttta
     8401 tgtaagtttg catcgatatt cttgtaaaaa ataattaata atatgctggt aaatattctt
     8461 ctcttttcgt taggcgctcc tgaagtaata cttattgctc ttgtcgtttt gcttattttt
     8521 ggtggtaaga aaatacctga actcatgaga ggtcttggca aaggtgtcag tagctttaaa
     8581 aaaggtctta aagacatgga cgatgaaatc aagaacgatc tgaaagacga ttctgaaaaa
     8641 taagctctat tttcacctgt ctgacattca ataagtttcc aaaaattggg caagcctgat
     8701 tccgaaaaag aattcacctt ctgggagcat ctcgaagctt taaggtggac aatactcaga
     8761 tccctgggtg ttgtcactgt gttgacgttc gttttttttg gttttaagga ttttgttttt
     8821 tccaaaatca ttcttccacc actcaattcc gacttctata cctaccgttt gctttgcttg
     8881 ctgagcgaaa agctgaagat gccaggcttg tgtccggatg atttttcagt tcagctcatt
     8941 aatatcaatc ttgccggtca gtttataacc cacatcacct catcgcttat catggcagtg
     9001 gttttatcta tgccttacct gctatttgag atctggaggt tcatcaatcc ggcattgtat
     9061 gaacatgagc gcaaaaatat tagcatcatt tttctaagtt cttctttttt gttttatctg
     9121 ggtgccctgg ttagttactt tcttattttt ccacttacca ttcgtttcct gggaacctat
     9181 caggtatcgg gactggtaac caatcagata tccatacaat cgtacatgaa tgccctgggg
     9241 atattaattt tcagcctcgg aatcatgttt gaattaccga tagtgattta tgcactaaac
     9301 cggattggac ttgtttcaaa aaaagcactg aaatctttcc ggaaatatgc ttttgtaggc
     9361 attcttatca tagccgcagt aattactccc actactgatc cctttaccat gctggtagtc
     9421 gcgctgccca tctatctgct ctatgaagta agcatccttt tatgtaataa agatcttccg
     9481 gcacttgaat cagattcaga agaagaagac taaatctttt tatcctccag aaattcatgg
     9541 catttcaggc atcaacttgc cgtattatta ccccaatttc aaaccatcga tgatatatac
     9601 ttttgatttt ctattattta agcgtatctt tgcaccctga tttccacaag tattaacaga
     9661 ccattcttat tctctaaatt atcaattcaa tgcaattttc cgatttcaac ctgaacaatc
     9721 cattgattaa cgcgctcaat gatctgggct ttaccgaagc tacaactatt caggaaaagg
     9781 cttttccggt catcatgtct ggtcgcgaca tggtgggtat tgctcagaca ggaacaggta
     9841 aaaccatcgc atacctgctt ccctgcttgc gccagtggaa gtttaccgtc gaaaaacatc
     9901 cccaaatttt gattgtagta ccaacccgcg aacttgttgt tcaggtaact gaagaaatta
     9961 aaaaaatgac aacctacatg aatatccggg ttgcaggagt ttatggtgga gaaaatatga
    10021 tcaggcaagc gccgcttatc cttgaaggat tggatatatt ggttgccaca ccaggacgtc
    10081 tgctggattt tgcacttaac gggacactta aactccggtt agttaaacga tttgtcattg
    10141 atgaagtgga tgaaatgttg aatctgggtt ttcgtccaca gctcatccga ataatggatt
    10201 tactgccccg aaaaagacag aacctgatgt tctcggcaac gatgacaaca gagataaagg
    10261 acttcatagc tgatttcttt attgatcctc aggaaataga agccgcacgt accggcactc
    10321 cgcttgcaaa tatcaaacag ataggaataa aagttccgaa ctacaataca aaaataaatt
    10381 tactcgaatt cctgctgaaa acagagcctg gattttcgaa agtattgctg tttgtatcaa
    10441 ccaaaaaact agcagacgac gtatattcac gaatctccac acttttcccc gaaacaatcg
    10501 gtgttattca ctccaataaa acccagaata atcgtttcga gacactgaat tcattcaaca
    10561 aaaatgcact tagggtatta atagcaacag acattgtggc acgcggactg gatttcgacg
    10621 acataaccca cgtagtaaat ttcgatgtgc ctgaagaacc cgaaaactac atgcaccgca
    10681 tcggtcgtac aggacgtgtt gacaaaaaag gggtggcagt aacttttatc actaaagatg
    10741 atactaccaa acgcgaaaac atccaactgc ttatgggaca aaaaatcccg atgatgcgca
    10801 tgccagaaga agtaaaaatt tcaaccgagc ttaccgaaag tgaacttcct caaattataa
    10861 caggaaactc cgtggcattc gtttcgcgga aagaagatca cggcccggct ttccatgaaa
    10921 agaaggacaa aaacaaaaaa gtgaatatga aaatccgccg taaggaagcc ctgaagttga
    10981 aatacggaaa accaaaaaca agaggacaga agaaaaaata acccgttctg tttctcagaa
    11041 tttgatattg gccagtttca gcatcacatt gcctttcaga ttggcctgaa tacgtacagc
    11101 tcccggtacc aggcttacac tcttcatggt aaaatcgtcc agttttccct gaagactaac
    11161 accttcatag actttataat ttttgagcat gacgttggct tcggttttca tctgatcggt
    11221 atattccttt acaggaaaag ataggtaagg aacaatcttt ttcagtatca gaccatgcag
    11281 caaccaatcg gccgatttaa tgagcgtatt ctttgttttc agctcaaatt ccggattgac
    11341 cacttccact gtcattttcg aaggattata aatcatatca ccggtgaaat aaatacgtcc
    11401 tttgaccgaa ccaataacat ctgccgaaaa aacagctttc ccctcactgc cgaaaaccga
    11461 taaatctgta attttaatgt actttttacc ctggctgaaa gtttgattaa gcagttgctg
    11521 tttggcaagt tcggatatct tgtcgaaggt cacatcggaa gctacgttca gattgaactg
    11581 ctgtggcgca ctgttggatc gtttgaacgg aggtaacgct accggcttac ctgctgctgg
    11641 ttttgagccc ataatggttt ccacgcggcc ataaagcgaa atatctgtct gaagtttacc
    11701 gcccttagta acaaaaggag ataccagaat gtctttggga ataattcgta cccacacatc
    11761 attttcagca cttgcctgta cgggtttctg aagttcagtc caggcagcag ctacctgttt
    11821 attcagattg gcaaattcac cgagcgattt atctatctgt tgcgtaatgg tcttaccgta
    11881 tttcgagagg gccagatcgg cgatgggttt taccggcaca tttacaccaa tcatattcag
    11941 tttgggtgtc tgaatccatt catagccctg cgaagcagtt tcggcaacga ggttccagtt
    12001 tttgtcaatt cctactgatg ttttatactt cagcgcgata attcccgtgg cttcgtaatc
    12061 gtcggcaagg gtcacgccca gtttttgaac cttccaggca aaccgcgacc agattttgag
    12121 aggaatacgg tactctatgt tgttattgtt tacagtaaag gtaaagtcgc cggatttcca
    12181 gactttgatt gacaaatcct tgtccgtaat atttgatcct tcgtatagca gcccattcag
    12241 gttgttgttg acagaagttt caagttgttt gatatctaat tccagatgca gcggaagttc
    12301 cgaatatgca ggtaaaagat ctgaaggtaa gtatgattct tttggacgtt cggttttcaa
    12361 ggttttacac gaagaaaaaa ttaatgcgac aaagagggca attaataaca acgatttatt
    12421 attcatatct gctattttta ctactattca gtttgttttc aattagtgtt ttttcttttc
    12481 aatccgggta tgtgttttca gatagacggg cagagcagcc gatccatacc agtcataaag
    12541 ttccacctcg aacacctttt cgcggactgt gggatcggac tgaagaagtt tcagagcctc
    12601 ttcttttgat tttacatcca gaatgaaaat gccacgatag ctgtttgggt ttttcccgag
    12661 tggtcctgcc acgatcagtt taccttcctc cgacaaacgc tggatattgt tcatgtgtcc
    12721 gcgaaagagc gtatcaagat cttgtttgtc ggttgtatta ttgggtccgg ttttgagtat
    12781 taccagaata tagttcttca taccatattc atcagctccc agctttttag caagccgtgc
    12841 atcgtagcgt tgcgggaaag caacgcaaaa gacgctcatc aatataatgc tgataattaa
    12901 acggttatac ttcatacggc attttttgct attattgaag ttcaatagta gtttcttcga
    12961 tattcagtac attattgagc gaaacttcgg tggtcatgtt actgtatatt acccagccgg
    13021 tattaccatg tatttgggag gcaatgcgtg tttcattgac catcggtgga aagtcaagct
    13081 gctcgttttg aatatcaagg ttcatatctg aagcaatatc ttttacaatt ttctttgcaa
    13141 attcggtgag ctgacctgag tctgcggttt gccacgaccg gattattgaa taatcgttat
    13201 cggtatctat ttcatcgagc agcacagtaa gcattccgtc gaaaggttcg cctccgtatg
    13261 catttggtaa tttaacaggt gcgttcactt cttcgcccag tttgtatttt cctccatgaa
    13321 acgaataaaa ctgatttatc agtttgacgg atgaaccttc aatcgcttct tttgaattta
    13381 acttactttc caagccatta atcatattaa ccagttgtgg tatcgtatca aattttgttc
    13441 gcaacatttc aaacgctttt tggttttgtg cctgcatttc ctgccagttg agtatttctt
    13501 taaatacacc ggtttcagat accctgaaaa taatctttga attttcgcct atcttataca
    13561 aatcatccaa taacgggttt ggcgtattga gcacctttac atcatgcgat aaccattgaa
    13621 tagtgtagga atttgctgta gaatcaagca cttcaacgtc gagcaggtat gctatctttt
    13681 gtttacttat cgtgtctgta gcgttttgga tcttgtttga ttcttcggtt acctgatagg
    13741 tttgcttatc atgtacattc cagtaaccca ctacctgaac tgttgaatct gctgcattta
    13801 tctgaccgaa aagtgaaaat ccgcaaaata caaataaaag aaatacagtt atttttttca
    13861 tttgaaaata atttatttat cacattgtca aatggaactt gcatgccgaa ttcttactgt
    13921 atttgttgaa tattcgtatt aatgctcgtt tcatgaaatc aatttttcag aagtaatttc
    13981 gaataatttc gggttgtaaa attactcttt ttttaagaca aaatatagca actacgtttt
    14041 aattcaaagc cgccccggaa atttataaaa tcatattggt ttacaaccct atcactttgg
    14101 catcgacccc aaatacaagc tttgtatcaa cgacatattc ttctgtcgta gctcttacag
    14161 ttgtaacttt cacattcagc tttatattgt ttttagtcac ataatccttc aggtttacct
    14221 tagagtcaac atccagattg agtgaattac caacattggt gttaacatct ttttttgagg
    14281 ccatcaaaac aggttcaagt ccgtcggctg agatatagac ttcaactgat ttgagaaaac
    14341 taaaatccgc agtttgtggt ttatttattt tgaatgtagc acttttaata caaatgcttt
    14401 caactaattc tgtgttgatg ttaaacattt cgaaataact catgtagtta gtctccacgt
    14461 cgggggttga aaacgaaaag ccattcatta cgggcaatga tcccggaaga gtaaactgtt
    14521 ggttcaccgg aaggtcaaac tgtgttaatt tatcaacctc cttgcacgac ggaagaattg
    14581 ccatgaataa tgcaaaaaat aaaaacaact ttctcatatc tatatttatc tgtgtgttaa
    14641 taacaaaacc agttcagaag ctaataaatt cattcatcag ttccacttca aatacttctc
    14701 acaaaaatat aaaaacattt tcaactgact tagtccacac atgagtatta agcacaaaaa
    14761 aattcgtctt gaagttaggt atataacacc ttcaaaacga atttgtagta tttgacgcaa
    14821 tttatttctt ttctttcaat aaatcacgga tttcagcaag caatacttct tctttcgatg
    14881 gagctgctgg tgcttcagga gcaggttctt cttttttctt aaatgaattc atgcccttga
    14941 tagccagaaa gatggccaaa gcaatgatca ggaagtcaat agtaacctga ataaagttac
    15001 cataattcag tgtagcagct gctttttcca caccatttac tacttcagcc ggtctaatta
    15061 cccatttgag ttcggtgaaa tttacacctc caagcaacag gccaattaat ggcatgacaa
    15121 tatcagctac aaatgatgca ataatttttc cgaatgcacc accaatgatt accgcaacag
    15181 ccaggtcaat aacattgcct ttgattgcaa attccttgaa ttctttaata aaacccataa
    15241 ttataatttt taagttaaat actttgaatg caaaaatata catttaactt taaattacaa
    15301 gtatattgta atttaaattc tctatatttc attaaatcta tcaaataatt cctcctgctg
    15361 catcgtaatc acaccaaaat gtatcgtttt agtatctcag agttttacag atttataaat
    15421 ttataacact ctatgacaga atgatactaa aatctctaaa gactatattg aaattgaatt
    15481 atcgataaat gaagcccagt ttcgttaaac ctgctttcac atctggacaa ctcataaaat
    15541 tattccatat taatccggtg cgataattct caatcatgac gataatgggt ccctgatcaa
    15601 tggcaagata cgattcggca aaccagcgcc gcgacaaaga aaaagcatct ttaaatccat
    15661 attcacccca gacgcggtta cccagcgtgt aataaaagaa attcaacgct ttcgtgcttt
    15721 ctaccggagt gtaaggaaaa gaagcaagcg cagctgacgg cgtaattgtt ccctgatcgt
    15781 tggtggggga tgaagctgtg tagccatcag ggaaatcact ggcagtaagc ccccagcaat
    15841 tttcatcgta accgtagtag ccctttggat tggctacgca atacttgtag ttaatgctgg
    15901 tgtgagcctt attttgtatc cagtaactgg catatgtgtc ggataaattt cgaggatcga
    15961 gtcccaggaa ggaatagtga gcaaaaaaca agggacctcc ataagcttcg cccaacggta
    16021 aggtaacacc ataatacgaa tttccgtttt tcattgctcc gttacgtgcc cagccttcat
    16081 catacacccg tttagggatt ggatacgtgg gcgatgacgc ggcaagtaca taagctatca
    16141 atccttcgtt ccagcccgtg atgggcatat tcatatccca gttgtaatta ggcgaccagt
    16201 gccagtagag tttttgttcc ccgtttttct gaaaccagtt ccattccacc ttttcccaga
    16261 gttgttgtat gatggcacac atagcttgtt ctggcggatt acccgatttg aaataggcct
    16321 gaacagcaag taaaccctgc atcaggaagg aggtttcaat cagatcggct ccattatctt
    16381 tagtgctgaa cgggataact tttccggttg ttccattcag ccaatgtgga aatgctccgt
    16441 gaaatttatc ggtttcgggt ctgttaagaa aacggactat tttatctatc tgtgcataac
    16501 cctggcttcg tgaaataaaa ccccgttcga tacccacaag aattgccatc agaccaaaac
    16561 cggagcctcc ggtggttacc gtttcgcccg aaccaagccg ttcgcgggca agtcccgaaa
    16621 cgggatgagc atagtcccaa aaatagcgga atgtttgttt ctgtaccaac gtgagcagac
    16681 tgtcggagga aatggtcggg aatttgggcg tggtatccag ttcggtaaga aaattcacct
    16741 tactgttgtc tattatatta acgcccatat attcacctga atttatattg agggtgtatt
    16801 ttgtataata attaagtttg tccttaagtt taatctttaa ttcttttgga ttttctgtag
    16861 tcaaaatatc aaattgaact tcctcattat tggtaaaata gattttatcc ggtctgattt
    16921 ttgaattgtc aatctcggta gaaaagatta cccggataat taccgaatca acaggcacat
    16981 tcatgactat accatcggat gccacgcttt taccgtttac cgataataat tcgatatacg
    17041 cattggaaag cagtaccggc ggttctttac aagaatagaa aaaagaaata acggacagtg
    17101 tggtgagaat atataaaaat tttgttttca tcttattaca aactataaac tgtgggaaaa
    17161 atcccacagt ttatctttct taatcattaa ctacttactc aaatgatttt atctgcctgc
    17221 tttttcgagt tcataaagag ctccaatatc agccaaaact aattcacgtt tccatacgcg
    17281 tacttcgtcc agttgacctt tcaatccggt attccaggca tcagtagtag taccgttggc
    17341 aaatgtttca aatgctccga gaataggatg gcaaggagtt gtaacaacaa agtttttagt
    17401 acctgtatta tctctgtttt cccaaaccgg attagatatt tttataccgt ttacatacac
    17461 tttcagaagt tttgttgtat tattccaggt aagaaccacg tgtgcccatt ttcctccaat
    17521 tttgttagga taagcaatat gaccagctgc aatatcggct gcacttgctt taacagtgtt
    17581 tcttgaatcc tgccatccca gatctgtact tgaaacaata agacctttta cggttaagct
    17641 gtcgcttgtt gctggcatcc agccggtttc agacatgaag ttgatacttc ctgcccactc
    17701 gttaggacgt gacaaagaga atatgacgga tgaatttgct ccgttattcg ttagtttcac
    17761 ccaggaactt accgtaaaac cagtattaag attggttcct aagttggcta tggtattata
    17821 tgccagatat cccacattga agcttgcagc tttacctttt attccatcta cccaggttac
    17881 attacttgtc ttggcaggag ctgaacttga caatgattcg ttaccatcac catttaatgg
    17941 ccaataagca accaaatctg cttttcccac ttcatcagca ttatcgtatc ctccaatggg
    18001 tggaagtttt ggttcgtctt ttttacatcc agtaaatgta aaagcaaaag ttgcgaatgc
    18061 aattaatcca aaaattttta atgttttcat tttttttgtt tttaaatagg ttaataattt
    18121 gatttttagt atattaattt attaattgat atcctaatca taatttggat tctgagtaag
    18181 tattcctttg cttaaagtaa tttgttcttc aggtatagga aacaattcgt gttttccctg
    18241 tataaaaact tttcctacag ctgacattgc aacagaagcc tttccggtac gtacaatatc
    18301 aaaccatcga tcgtgttcca ttgccatttc caatcggcgt tctttataaa tctgatctaa
    18361 tgtaacagag gtaagcggaa tcaatcctgc acgttttctg attttattaa caatggataa
    18421 tgcagtatcc aactgattaa tgtggtatcc tgcttcggct cttatcagca aaacatcgct
    18481 gtatttcaac aatcgaacat ttttggcagt ttctccttta ttgtcgtccc aggactcctt
    18541 tgtgcggctt tgatatgctt tataattgta ccttggattt gcccagcctg tagcaggagt
    18601 ttggaatcca tcccaaagta cctgtccggt aaacattatg gtaccgtctt ttcgtttatc
    18661 acccggctcg taagcatttg ccagattgac tgttggtgta ttaaaacccc aaccgtggtc
    18721 aggcgttccg cgtccgctct gtacatcggt atatccgttt acacctttgc ccaacgttgc
    18781 ctgaatttca aaaagcgatt ctttactatt ttcccctact tcacgccaca cattggcgta
    18841 atcgggtaat aaatcataca tcttgcttgt tactacgttt cctgccatag caaatgcttc
    18901 gttccatttt tcctcataca tgtatacttt agccaaaagt ccctgtgcag cacctttggt
    18961 gactctgcct aaattttctc tgttgtattg caatggtagt gaagctgcag catccattaa
    19021 gtccttttcg atttgagcat aggtttctgc ttttgtttta cgtgaataaa ctacctgatt
    19081 tacggtggct tcttctttaa aatccagttt ttctaccacc acaggtacac caccaaaaca
    19141 tcttaccaga tcaaaataaa acattgcccg aaggaatttt aattcgccta tatagcgatt
    19201 tttaatattt tcctccagat ttaccttttc taaataatat ataccttcgt tgcagcggta
    19261 aattccttcg taacgtcttt tccaaacatc gttgaatgaa cgcaatgatt tatcaaaagc
    19321 cagatcgtca agtttgtctt tatctgttcc tgcatcacca tccgatgagc ctttgtcggc
    19381 atcatccgat gttatgctcg ttacgccaat ccaggggaag ccatacatct caaaactcaa
    19441 catttgatta taagcagagt ttacaacttc tacagcgtac aatggttcgg caagaaaatc
    19501 atctttcgac atcttatcct gaggaagtac attcaaatcc ttttcgcagc cgactaagaa
    19561 cagtgtcaag caaaaagtaa ttatagaaag tattttaacg tgtttcataa tttcttgtgt
    19621 tagttacttg attaaatgtt tgatagactg ttttataatg ttatattcag accaaaagta
    19681 agggatctgt ttgtgggata agcatccaac tcaatacctg cagaaccgag cggtgtaccc
    19741 gaaccaatca attcaggagt aaatccggta tattttgtga aaataaaagg attaacagcc
    19801 gttacataca ctctcaattt ttgaatttct tttagcactt tcggcagaga ataacctatc
    19861 gtgacgttgt taattctcag ataatctccc ttttcaataa aataagtaga agccaacgga
    19921 gtatcagaat atggtctggg atttgcagca tcctgttttg gatttgaagg aatccaaaaa
    19981 tcattaaact cattcatttc cacattttcg ccgccccagc gttgcgcttt tttaccattg
    20041 tacactttat ttcccattac accataccaa tccgttgaga aatcaaaatt cttgtaatca
    20101 aatcccagat tgaaaccata agtcagtttc ggaatatacg aaccggcgtt tattctttcg
    20161 gacgaataag caaaagctcc atcttcagta tagcctgtat gttcatatac atagaacgat
    20221 ccaatcgggt caccaatatg tatttcttta gtataatgtc cgttacccag atctccaccg
    20281 gtaagtttat caaaataaga actgtagatt tgttttatcg cattattgtt tactgcaaaa
    20341 tttacacctg ccctataatt aaactcatcg cccaatttat cattccagga gaatgtggct
    20401 tcaatcccct tgttttcaac cgttccggca ttcaaataaa catctccggg agaaagtact
    20461 gttggaagtt ttacaggtaa aattacatta tcggtcaatt tacgataaaa ttccagggta
    20521 ccgtataatc gttgtttcaa cattctgaat tcaataccgc caccgaattc ttttgttgtt
    20581 tcccaggtta aatcaggatc aacggcataa ggaacaaacg aaccgggata aattatttca
    20641 ggtgagccaa aaggataagt ggcaccgttt tggaaaagta tctgattgac agatggagcc
    20701 gcgtttccgt taccaatttc accatatccg gcttttattt tcaaataatc aattactgaa
    20761 gcatattttt caaaaaaatc ttcgctgctc acaacccatc ctcccgaaac tgccgggaaa
    20821 attccccatt ttttagccga ttgaaaatca ctgataccgt ctcgacgaag tgatgccgtc
    20881 aataaatatt ttcgctcata atcataatca acacgcgcaa aataacccag attgacaagc
    20941 ggagtttgac tactgtttga tactacatta tcaggtgaag taacaatatt gcttttcgac
    21001 aggtcaagcg accagtaatt aggttggtaa ggtacgttat tgcgccttcc ggaaagatac
    21061 tgtgcggttc tggtagtgga acgtgtaaca cctaatacag ctgtaattcc atgtttatca
    21121 aaatctttgg aataggtaag aaaattatcc cagttccata caaaaaaatg acttctgctt
    21181 tgacttaacg tgctgtagaa cttattgttt ttataaaaat cgtcttcagt cgcagttgga
    21241 ttttggctga accaattttc tttattcggt gtgtaagaat atcccatacc ataatcaaat
    21301 gtagcaccga agttggaatt aaatttcaga tccttgtaaa tttgtagttc tgcccccaca
    21361 gttccgaata aagtgacatt tctgttttta tcttcgttgt aaccaagcat ggcaagggga
    21421 ttcggtacat tattaaattt atctcctatc atacctattt ctccggtagc attatccaga
    21481 aatggcattc cccattttcc gttttcatac ttcacgggca ttattggcga ttgcttataa
    21541 gctgctgtaa atgcacttga aggttgtggt acgttattta caacggcaac attgatggat
    21601 tgagatattt tcaggcgatt gaaaagtctg tattcatttt tactcagaat gttggttctg
    21661 tcataatcag ttcctttcag aatcccttgt tcgtcgaagc gtgaaatacc gaaataataa
    21721 ttcacgtttt cttcaccacc ggaaagtgaa atggagtgat tagttgtcat tccggtttga
    21781 gttatttcct tgagccaatt cgtatcgtaa ggctgagtgg aagcaaaacg ggttctccct
    21841 gcggcaaaat tctgataata tgcaaaatga tatgcatctg ccatatctac tgttttcatc
    21901 attgttttga aaccgacgta accgtcgtat atcactttaa cacttccttt tttccctttt
    21961 ttggtagtaa ccagaattac accacttgca gctttgtttc cataaattgc gagcgaagaa
    22021 gcatccttga gtacatctat agtttcgata tcgttagcac tcagcccatt aaggctattt
    22081 gcttcagtgc cgtcaattac atataatgga gttcccccac ccagtacagt attaatacct
    22141 cgtatcatca cagtcggtgc ccgtcccggt tcatctgtgg caataatgtt aacaccggct
    22201 gcttttccct gaatagattg aatagcagat tgtgctggag ttttcattat atcatcagat
    22261 ttaatctgaa caactgatcc tgtaatggct ccggcttttt tagtaccgta accaactacc
    22321 actacttcat ccagcacctt gtcgttagtt tccagttgga tattgagtgg agcattggat
    22381 ctaaccgaaa cttcctgact tttatagccg atatagctga tttccaggat tgaatttaac
    22441 ggtacggaaa tagaaaaatt cccgtcaaaa tttgtaatcg taccgttatt ggtaccttgt
    22501 tgcaaaactt tggcaccaat caatgtttca ccggtctttt tatcagatat tacccccgaa
    22561 atagttacgc tttgggcgac aatagagaaa accgaaataa acatcagtaa aaagattgtt
    22621 gtacgtttga ttttcatatg tactacttat tatttgaagt taattgaaaa ttaaatccta
    22681 atttttttaa cccgttttga atttcccggt ttcccatgaa taaattccaa acgagtccgc
    22741 tgcggaagtt ctcaatcatt actgctatag gcccctggtc aatggcaaga taacttttca
    22801 ccacttgctg accttcaatt aaaccgggat tgtaagaatc ataaaatccg tattttccaa
    22861 aaacctgttt tcctttttca aagtacagat actttaatac ctccatactc tcctttggag
    22921 tataaacaat ggatgaaaga gctgctgtag ggggaacagt tccgttatcg gaatcggttc
    22981 cgggatgatg cgacgaatat ccgactatcg ggtcgtccga cgaagtaaat ccccataaat
    23041 tcgctccata gcccgtgtgc ttttttggat tattgatggc ataagcccta tggataaggg
    23101 tgtgggcttt atttctttca aagtagtttg tatagctgtc cgtcaatcca ttcggattta
    23161 gtcccagaaa actgtaatgt gtgaaaaaca gcggaccacc gtaatccatt cccaggtcaa
    23221 gttttattcc ataataactt ttcccgttgt aataataagg cgaatttttg aaacaggctt
    23281 catacacttt tgcatcaatc ggatatgtgg gtgatgatgc tgcaagcaca tacgtaatca
    23341 gcgtttcgtc aaatcctttg atgcagtggt tcattttcca tccgtaattt ttagaccaat
    23401 gccagtacag agagtcggag ccctgtgtgt accagttcca ttccaccgct tcccaaagat
    23461 gtgtaatttt attcgccaat tcatgttctt tatcatttcc ttttttcaaa tattcacggg
    23521 cggtgagcag accttgcatc aataatgcag tttccaccaa atcgccacca tcgtcatatt
    23581 tactaaagct gaatgctttt cgtgtatctg cattgtacca gtgtgcccac gccccatgaa
    23641 accgctcgac agagccgaga aaactgacaa tcttatttat aacctcaaaa cctttttctt
    23701 tgggaaaata atttctttcg attccggcaa tgatggccat cagtccgaaa ccagttccac
    23761 cggtagttac tatatcgcca ttcacatcat tgctgcgttc acgtgccatt ccggttcccg
    23821 gctctgcaaa atccaagaaa taacgggttg tataacgctg cgtcatatcc agcaaatctt
    23881 cgtctctgta agcttttcgc gttactttta tttcaaattt ggcagcatcg gaatcggtaa
    23941 ctttcattcc cttcggaata attctgtaac gaatttcagt atttggggga atatttcttt
    24001 cgtaaaaatc gagaaaatta tcctgagtag tttcaccctg ttttacaaat gattttgcgt
    24061 ttcctactag tcgatacact tcatacgtaa aaccctgatg gtttttccag caaatttctg
    24121 aatggcccgg aaagctttga gctttcagtt catttggcac aacatattgg ctttgagcag
    24181 acaatactcc gaatatcatc aaaagtaatc ctatactttt tagctttttc ataaactatt
    24241 tttgccaatt aatagttacc gatttcacat catgtgaatt cgtgccaatc attatttcaa
    24301 aatcaccgct ttcccaatcg tatttcagat cattgttata atacttaagt tcttcaggtg
    24361 taatatcaaa gcttacagtt ttgctttcgc ccgctttgag catcactttt cggaaacctt
    24421 tcagttcctt taccgggcgg gtattgcttc ccaccacatc gcggataaaa agttgaacca
    24481 cctctgcccc atcgtatttt ccggtattcg ttaccgagat acttgctttc aacgtctgat
    24541 taccctgcaa ttgggtattg ctcagattaa tatctccata actgaatgtg gtataactca
    24601 gcccgtaacc aaacgggaaa agtggagaat ttggaacatc gagataaacg gatcggaatt
    24661 tatcaaactg atcctccttg ttttcgggtc tgccggtgga tttatggtta taatatatcg
    24721 gcacctgtcc tacattccgg ggaaatgtca tcggcaattt gcccgaagga tttacttttc
    24781 caaaaatcac atccgaaatg gcatatcctg cttcactgcc tgcaaaccat acattgagaa
    24841 tagcatctgc cagtttactt tcgtcattca acaccagcgg acgaccggtg aaaagaacga
    24901 gcacaatagg ttttccgagt tttttcagct caacgagcaa atctttttgc gattgaggta
    24961 cttcgatgtt cgatctcgaa cttgattcgc cactcatttc ggctgtttct cccaatgctg
    25021 ccacaattac atctgccttg cgggcaacct ccacggcttc tttcagtatt tcttcctttg
    25081 gccggttatc ccggtatgag ttcttgccaa acatagagat acggctttca aactgagcat
    25141 cgtaatcaaa attagctcct ttggcagtta caaaatttac tttatcaccc agtgtttctt
    25201 tcaatccttt caacatgctt acagctttgg catgttttgc agcaacactc cacgtacccg
    25261 gcatattttc aggattatcc gccagcggac ctattacagc cacagttcct gaggctttaa
    25321 gtggtaaaac attgttttca tttttcatca acaccattga ttgagcggca atacttcggg
    25381 cttccgttcg gtgctgatta ctgaaaatct ctgtttttgc ccggttggca tcgcagtatt
    25441 tgtatgggtc ttcaaacaat cccagtctgt attttgcttc gagtatacga cgcactgccg
    25501 cgtcaatagc tgaataactt acctgtcctt ctttcaatga tttggtcagt gttgtcagaa
    25561 atccctcacc caccatatcc atatccacac cggcattcag tgccagcttc gatacctgct
    25621 gcaaatcgcc cattccgtgg gcaatcattt cattgatacc ggtataatcg gtcacaacaa
    25681 atccggggaa ctgccattga ttgcgcaata aatcggttaa tagccatttg tttcccgtag
    25741 caggaattcc atctatctcg ttgaacgaaa ccatgacact cccggctccg gcatcaactg
    25801 ctgctttgta aggcgttaag tattcgttgt acattctgac cctgctcata tccaccgtgt
    25861 tgtaatctcg gccggcttcg gctgctccat acagcgcgaa atgtttaacg caggcaagca
    25921 tcgtgttgct ggcagataaa tcatttccct gatagccgta aaccattgct tttgcaatct
    25981 gacttccaag ataagcatct tcacccgagc cttcggctac gcgcccccag cgcggatcac
    26041 gggcaatatc caccataggc gaaaaagtcc agttgatacc atcggcagtg gcttcctgag
    26101 cggcaatctg tgctgatctc ttaatcaact ccatatccca cgatgaagca agtccgagtg
    26161 ggattggaaa tgttgtttca taaccgtgaa ttacatccat accaaaaatc agcgggattt
    26221 tcaagcggct ttcttctata gccacacgct gcatttctcg gattttatca acacctttaa
    26281 tattaaacaa tccgccgact cttccttctt tgattttctg accgatgttg gaactttttg
    26341 cctgccctgt agtgaaatca tctgcagacg gtagatttaa ttgacctaac ttctcatcga
    26401 gtgtcatttt cgacatcagg tcaccaatga attgatccat cgctggattg ggtttgaaat
    26461 gagtcgaatt cgttgaaaca acctttgttt gtacaggttt acttacaata tcaactggcg
    26521 ttttttttat ttttttttgc gcgtatgctg agttcacagg cgttaaacca atcaacatca
    26581 taatcggcaa aaacaagcaa aaataggagg tcttcatagt tttataattt ctgtaacaaa
    26641 tttgcacacg aaattttcta attcaaaata tttcaaccct atttgaatac tacaattaaa
    26701 aatacctaaa aaaggtagcc ctattttaac cctacacgat tcaactaatt attcatatat
    26761 atctcatatt gtgataatta gaaagtagtc attttgtaaa aactgtcaga aaaaaaatta
    26821 aaacaatata atagtattta cttgaactca attaaaaatt cgctgatatt tttttcggat
    26881 ggaatgttca tctttttacg aattcgataa cgggctactt ctacaccttt aagtgaaatg
    26941 ttcattaagt gagctatatc ctttgtgctt aaattcaaac ggaaaaaagc acaaaaccgc
    27001 agatcattag ctgttaattc ggaatatgta acgtgtaagt ttctaaagaa attttcatga
    27061 atcctgtcaa aattagcttg aaaaatcgcc caatcatctt cagaagaaat attttcatcc
    27121 agaagtttta tcagtttatc ataatatttg tttggatatt gtgttcccaa tttatttttt
    27181 aaagaaacaa tttcatcttt gattgtgttc atcagttcat ttttctttat aatagtcata
    27241 gttgatccgg caagctcttt actcttctga acgatttccg attccaattt ctcatttttt
    27301 aatgctataa tctgttgttc tcttttttca atctcctttt tgcggatagc ttcctgttct
    27361 tcatgaatct tttgtttttt aattctgaaa ttacgcttaa caagccagta agcaaaataa
    27421 accatcaggg taagaagcaa aatatacagt atttttgaaa atgtattcca gtagaaagga
    27481 ggtcgaacca gaaaatcata cgaaactgtg gaaagtacgt cattgctgtt ggacagtgcc
    27541 tgtacttcga aggtatattt tcccggtgac agataactat attctttaga attttccggg
    27601 gtagatctgc tccagacatc atccaatcct atcagtcggt atctaaaaga aaggttattc
    27661 aaatgtgagt aaacaggata aaaaacggaa aatctgatgt tgttgttttt ataagatatc
    27721 tttacctcgg aatcggctac gacaggaaaa ttaattatct tcctgttttt tctgtcactc
    27781 acttctactt ttgaaatctg aagtttatac ttactcaaat tcattttacc gttttgtctt
    27841 tcatagagtg ccaatccatt ttcgagagtt attaacgaca ttttatcttt tagataaaaa
    27901 atattttgat aattatccac ggtctgattc agaaacaatg aattctgtat ctggtcaatt
    27961 atattgattt tatcattttg tattttcacc agagcagctt catcattacg gataaaccag
    28021 taataacttg gcgacgaact gcaaacccga taagcctgtg aaaatcgtcc aagggagtta
    28081 ttaatcaaat cgtatggaat aattttcttc tgaatgtcgt cgtaagtgta aaaagccata
    28141 ttatcggtaa atacaatccg gttattgatt gagaatacat ttattggcac tttatgaaca
    28201 ttatcaagcg atttgtaaac tttcaggctc tctatcgttc taaggtcttc tttcaagcgg
    28261 atagcataaa gtcctgcatg caaatgagcc gcccagatag tccccttata atcaacttca
    28321 atatactgaa tgggattgag aaagttactt accgaatgac taaaaatcca gacattattt
    28381 tttttctcat aaatacataa ttcggtgtat gtaccctgaa ccagcacttc tttgccatga
    28441 ataattcctt tactaatgca tattccgccc ttcaccggtg aaactatttt actttgagtt
    28501 ttatccactt caaaagtctg ttcattgttt ccacataaaa attgattatc aaatctggaa
    28561 atgttccaaa cctgcccggt aattcttgga tcaagagtca cattttcgat tctttttgcc
    28621 gttatgttca attttgccct gtaaaatccc tgattagtgg ctataaacag attatcaggt
    28681 tcctgaaaat tgacagaata aatggctcct atggctggtg agaatgtatg aatataattt
    28741 aaagattttc caagccgcaa cattgaaata cctttatcca aagctagcca aagattattt
    28801 tcctgatcac agcacatacc gagaacagta ttgttttgca gcacattgtt tgtattaagc
    28861 gaccacacct tttgaccttt gtaattcaga gcagtcacac cgttttggat agttccgatg
    28921 accagtaaag aatcctttgt caacacagct ttgtttatat tagctcttga caactccctg
    28981 tcagtatccg tgattaaccg tctgaaatca ataccgttaa aaatgtagat tccatctgct
    29041 ttagttacga tgaaagcttc ataatgatta atcgggagca ttgctactac tgcactttta
    29101 aaaggagcgc tatttaccgg aataaaatct tccttctttt tgtcataata acataaccct
    29161 ttctgatctg tgtgagtata aatttcattt ttaaacttct ggaagaaaag aaaagtcaat
    29221 ggaaatcgtt tccctcttaa attatgttta tcataaataa aaaaactcgt gaaagactga
    29281 aaaataatct ttccgtcggt ttcaagtata ttccatatct catcattctg catgttataa
    29341 ttgcctagtt tatcggagat tgaagtataa accagcaatc ctttgttatc tttctcaaag
    29401 tagccaaatt cttcgaatga tccgacatat attttcttat cggaagaaat aaaaagtgaa
    29461 cgaataatct tgtttcccgg tattttataa actttccagt tagaaccatc aaactgcaat
    29521 aagccctggt tattaccaaa acacatcttt ccgtcagcag cttgtttaac atcccagttc
    29581 tggtttgagg cattataatc tttctttgaa taatggttga caatgggtgt aaattcttgt
    29641 gcaaagataa atatctgaat agcaaccagg aaagatgtta tcaaaattcg aattttccac
    29701 atattcatac tttatgttaa ttcattgtaa cgcaaattaa tcttaacgcg ttatgcaaaa
    29761 ttaaaataat tttacgatta ctgtatttcg aatgcatatt ttctaataaa aaagccctgc
    29821 agtaataatt actgcagggc ttttcagctt tataaaaata cgaattacta atatttttct
    29881 ttgctaagca ttattttttt aattcagctg ctgctttcag ttcttttttc ttcttctgac
    29941 ttaacatgat atcataagca accggtacag caacaaataa tgttgaataa acacccaaaa
    30001 tagagccaat taacagggcg aaaataaatc cgcggattgt atcaccaccg aagatgaata
    30061 ttgcaaacaa cactaccaga acacttgcag aagtactaaa tgtacggctt aaggtttcgt
    30121 taagcgcttc atttatcaca tctttcttgt ctcgtttcgg atataaccct gtattctcac
    30181 ggatacggtc aaaaataacc accgtatcgt tcacagagaa accgatgtaa gtcagtatcg
    30241 ccgcgataaa attcaggtcc atttccatgg agaatggcat gatgccccac aacagagcaa
    30301 atgagccaat aataattatg gtatcatgag caagacatag catagtaccc attgagaaag
    30361 caaagtggtt gaagcggatg aaaatatata atgacacacc gagcagggaa agaattacag
    30421 cccagatagc tgaagtcttt atatcttcag caatagttgg ccctactttc tgcgagttca
    30481 ctatatttt
//
LOCUS       DAOPYB010000019        28867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1616_length_28867_cov_8.313029, whole genome shotgun sequence.
ACCESSION   DAOPYB010000019 DAOPYB010000000
VERSION     DAOPYB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 28867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28867
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1616_length_28867_cov_8.313029"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..846)
                     /locus_tag="PKH58_02865"
     CDS             complement(304..846)
                     /locus_tag="PKH58_02865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX88000.1"
                     /translation="MKKSTVFLFAALLIVAITGISSCRKQAPQLPSNKVLAADTTGNS
                     LLEMNKILALREDSIIQAMIARNDMHFSKSKSGIWYNVLHSTRTDSLKNDSIISYSFT
                     TYNLAGEEINSGTRTIRPGKKEIPTGLEEGLLLMRKGETMQLIVPWYLGYGMNGNEEI
                     PPYTSLMYLIVNETTSPPAP"
     gene            complement(846..3290)
                     /gene="thrA"
                     /locus_tag="PKH58_02870"
     CDS             complement(846..3290)
                     /gene="thrA"
                     /locus_tag="PKH58_02870"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNX88001.1"
                     /translation="MKVIKFGGTSVGSVEGILSLKKIVESQQEAVIVVVSALSGITDL
                     LYATSKMASEGNKAYLTEYENMVSRHLELIENVILPEKKLQTIKLVQDNFTDLSNILR
                     GIYLIKDISVRITDTIVSYGEVISSIIVSNLIKGAVHYDARNFIKTNNQFDKHNVDFT
                     ISNQLIQDIFAETHDVCVCGGFISSDAKTGYITNLGRGGSDYTAAILASALKATKLEI
                     WTDVDGFMTADPKIISNAYTIERLSFSEATELCNFGAKVIYPPTIFPVYHKNIPVEIK
                     NTFNPEASGTFISSEKSSQLKNQQKAIKGISSINDTSLITIQGLGMVGVIGVNYRIFR
                     ILAKNGISVFLVSQASSENTTSIGVRNADSQLAVEVLRNEFAKEIQQGEINQIFSEDD
                     LVTIAIVGDNMKRTPGIAGKLFGTLGKNGINVIACAQGASETNISFVTDRAVLRKALN
                     VIHDSFFLSEYQVLNLFIIGVGTVGGNLLNQIKKQQKNLMVYNGLKINVVGVASEKKM
                     IFTREGVPLENCVERLKKEGQAANTQLICDEILKMNIFNSVFVDCTASAEVANIYQKL
                     LENNISVVTANKIAASSEYEKYVQLKETSRQRGVKFLFETNVGAGLPIINTINNLTNS
                     GDTILKMEAVLSGTLNFIFNTISSEIPLSKAIRMAKEARYSEPDPRIDLSGKDVIRKL
                     VILAREAGYKINQEDVQKNLFIPDPYFLGSQDDFWKMIPELDADFEEKRKELEKKGLH
                     WRFVATMDKGKCSVGLQTVGSMHPFYDLQGSNNIILITTERYNEFPMIIKGYGAGASV
                     TAAGVFADIISIANIR"
     gene            3586..3659
                     /locus_tag="PKH58_02875"
     tRNA            3586..3659
                     /locus_tag="PKH58_02875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3620..3622,aa:Arg,seq:tct)
     gene            complement(3714..4001)
                     /locus_tag="PKH58_02880"
     CDS             complement(3714..4001)
                     /locus_tag="PKH58_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88002.1"
                     /translation="MKDFVDYVEYTRPDNAKVRVAFEMLGNEVLFYFCEYQHTDKGEW
                     KLMESSDSEHFNYSVENEKGEIRDFSIKYPTKDEIEKAQQLIINKYNILSI"
     gene            complement(4071..5267)
                     /locus_tag="PKH58_02885"
     CDS             complement(4071..5267)
                     /locus_tag="PKH58_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNX88003.1"
                     /translation="MSEFSTHQINRLADLNILFDAITYKKQPAEAIKKYQHTIDNTIP
                     DDVVILVHKLMESSYPMEDIKSGINKLLNVLRRSLDSHPYTAPAYDTFFDICIRNNNE
                     VKNRLNKLRPFILQINKQGVTEHILTEMLRLWKEVQEFTQYYVIKENVLFPLVEKYLF
                     EYRCIAVMWSIHDDIRRNLNDLTDLLASGQLDLQKLNRLTGEVFYNIYSIAFREERLL
                     YPFIAKNVPASEIEKLWDESVEIGFPYFNPQKKIAEPSVKNVSSGLVDLKSGALSAEQ
                     LILIFSHLPVDITYVDENNKVCFYSTPKKRIFTRTNAILGRDVRNCHPHESVHVVEQI
                     VESFRNGTKDKADFWINMKGKKILIQYFAVRNERNEYKGVIEVTQEISEIQALEGERR
                     ILDWHQ"
     gene            complement(5344..6180)
                     /gene="pyrF"
                     /locus_tag="PKH58_02890"
     CDS             complement(5344..6180)
                     /gene="pyrF"
                     /locus_tag="PKH58_02890"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNX88004.1"
                     /translation="MTKQELFENIKRKESFLCVGLDTDINKIPEHILNNSDDPIFDFN
                     VQIVKATADLCVAYKPNLAFYESIGLAGWDALERTVDFIRAEYPDQFIIADAKRGDIG
                     NTSAMYARSFFGNMDFDAVTVAPYMGEDSVTPFLTYENKWVILLALTSNKGAFDFQLM
                     KDADSTDRLFEKVLKQSQSWGNAENMMYVVGATKAEMLREIRSIVPDHFLLVPGVGAQ
                     GGSLAEVAEHGLNNQCGLLVNSSRQIIYASSESDFKEAAHDEARKLQVEMSEILKNRG
                     LI"
     gene            complement(6182..6691)
                     /locus_tag="PKH58_02895"
     CDS             complement(6182..6691)
                     /locus_tag="PKH58_02895"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HNX88005.1"
                     /translation="METVIKYNKVKPVTLLIISLAFTLICIWLIFDSNRNSITGFAGL
                     TLFTILDVYIVYRLKKGGGIILNESGIVTDRNNPDDTFISWNEIERFSLYEANGIRTI
                     NIHLFNPGALIERQTKTNVRKNMQINQTLYNAPVGINPNLLDIKYKRLENLLMENLET
                     YKKNNITQH"
     gene            complement(6692..7780)
                     /gene="prfA"
                     /locus_tag="PKH58_02900"
     CDS             complement(6692..7780)
                     /gene="prfA"
                     /locus_tag="PKH58_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNX88006.1"
                     /translation="MAENSLLQKLDGLQHKYEEISMLITDPAVIADMKRYIKLNKEYN
                     ELDKIMNAVKEYKTALANITEAKEIYDKESDTELREMAKAELDNLEPQIPVMEENIKL
                     LLIPADPEDNKNVVMEIRGGTGGDEAAIFAGDLFKMYTRYCENKGWKVEVTDFSEGTI
                     GGFKEIIFTVTGENVYGTLKYESGVHRVQRVPVTETQGRVHTSASTVAVLPEADEFEV
                     DINPADIDFHTSRSGGAGGQNVNKVETKVQLTHRPTGIVVVCQQARTQLGNREIAMEM
                     LRTKIYNMEYQKHIDAIASKRKTMVSTGDRSAKIRTYNYPQGRITDHRINYTIYNLTA
                     FMNGDIQDVIDQLIIAENAERMKESELA"
     gene            complement(7823..8488)
                     /locus_tag="PKH58_02905"
     CDS             complement(7823..8488)
                     /locus_tag="PKH58_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88007.1"
                     /translation="MKNIEKKIITFLFVSLAFIAVQANTPYQYLKTAYTDGEFVTVCR
                     KEVNGNVLQSNAVILQMIEQLNNKPMKLFDWALKDLGLQGQKNDEMIIVLKKSLFDKN
                     TGITHGLVDVIIPNFQTIRDVKVDAVVKTELLPNGQLIGSVVVVNSGFLLKKAHATIT
                     SSHMKNGNQLLQTETHIQFGWLLKFFITQNRYKSIVEWRIDKFTENMQKEAEKNTYSQ
                     TIY"
     gene            complement(8573..9490)
                     /locus_tag="PKH58_02910"
     CDS             complement(8573..9490)
                     /locus_tag="PKH58_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX88008.1"
                     /translation="MTKESLKKFAYLSIAAAVLTIALKFGAYFLTGSMGLFSDALESC
                     VNLLAAVIALIMISISERPADKDHEFGHTKAEYFSSAIEGALILVAAFSIIWSAIPRI
                     IHPLPLENVGVGLLISTGASAINLAVALILIKNGKKRHSLLLEADGKHLMTDVWTSVG
                     VLVGIGLVKLTGLNILDPIIAVIVALNIIWTGVHLLHRSGNGLMDSVISPDKQKLITN
                     FLESQKEQGIEYHSLMTRQAGQRIFISLHLLVPGSWSVQKGHDKSEYIEEYIENLFEL
                     PATCSTHIEPIEDPASLNDIGIDRKKIKI"
     gene            complement(9588..13265)
                     /gene="purL"
                     /locus_tag="PKH58_02915"
                     /gene_synonym="purI"
     CDS             complement(9588..13265)
                     /gene="purL"
                     /locus_tag="PKH58_02915"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNX88009.1"
                     /translation="MILFFRTPENKIYAVQSLNLSDDDINKLIWLFGEAELLSEDKIT
                     GKFIGPRREMITPWSTNAVEITQNMAIRGILRMEEFFPVLDKDDHDPMLQRIYTELNQ
                     EIFTINKQPEAIRHIDDIALYNQQEGLALSEDEIEYLNSISEKIGRKLTDSEVFGFSQ
                     VNSEHCRHKIFNGQFVIDGEEKELSLFKLIKKTSEDNPNDLVSAYKDNVAFNQGPVIE
                     QFSPKSDEKPDFFETKNIESVISLKAETHNFPTTVEPFNGAATGTGGEIRDRLGGGKA
                     SLPIAGTAVYMTSYPRNAGKPWEKSLKERKWLYQTPEQILIKASNGASDFGNKFGQPL
                     ICGSLLTFEHNENDKTYGYDKVIMLAGGVGYASRRDALKGEPATGEKVVVMGGDNYRI
                     GMGGGAVSSVETGQYDNAIELNAVQRANPEMQKRVANVIRALAESDDNPIVSIHDHGA
                     GGHLNCLSELVETTGGKIEMDNLPVGDPTLSAKEIIGNESQERMGLLLQEKDIERVQQ
                     IAERERAPFYVVGETTGDMQFVFEQKDGQKPIDLLLEYMIGKPPRTVITDNSLEENFA
                     SVEVDEEQLLGYVQNVLQMESVACKDWLTNKVDRSVTGKVARQQCVGEIQLPLSDVGV
                     VALDYRGKSGIATSIGHAPLAALADPIAGSVLAIAEALTNIVWAPLKDGLDSVSLSAN
                     WMWPCKNPGEDARLYKAVEACSDFACSLGINIPTGKDSLSMTQKYGDEKVFSPGTVII
                     SAGAEVSDIRRVVSPVIVNNPETSLYYVDFSFDKLSLGGSVLAQTLNKIGNEVPTVTD
                     SEYFKAAFDSIQELINKNLILAGHDISEGGLITALLEMCFANSIGGLKVKLDDLSETS
                     LVKILFAENPGVLIQTANKAEVEKILKNNGIGFAQIAKPIAERRLEIHKKKTSYSFAI
                     NDLRDMWFEASYLLDRRQSGEKAAKARFENYKNQKLEFHFPQTFTGKLSQLGLSPDRK
                     PSGVKAAIIREKGTNGEREMAYALYLAGFDVKDVHMTDLASGRETLEDINFIVFCGGF
                     SNSDVLGSAKGWAGGFLYNEKAKVALDNFYKRNDTLSLGICNGCQLMVELNLINPEHT
                     NRAKMLHNDSHKFESEFVSLTIPENNSVILGSLSGCKIGAWVAHGEGKFSLPMGENAY
                     NIIAKYNYDEYPGNPNGSDFSVAGICSADGRHLAMMPHPERAIFPWQCANYPTERVQN
                     DEVTPWMEAFVNARKWVEGKK"
     gene            complement(13573..13824)
                     /locus_tag="PKH58_02920"
     CDS             complement(13573..13824)
                     /locus_tag="PKH58_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNX88010.1"
                     /translation="MGIISWILLGLIAGALAKAIHTGPDPGGWIITVIIGIIGAIVGG
                     WIGSFFGLGTVNEFSIKTLIVSTGGAVLILWLYSVLKKR"
     gene            complement(14125..14271)
                     /locus_tag="PKH58_02925"
     CDS             complement(14125..14271)
                     /locus_tag="PKH58_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88011.1"
                     /translation="MIIAGFLFVVIDNLIDNPEYANKISILKKELWNLQVQYDDSIRL
                     KNPL"
     gene            complement(14333..16165)
                     /locus_tag="PKH58_02930"
     CDS             complement(14333..16165)
                     /locus_tag="PKH58_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNX88012.1"
                     /translation="MLKNHPKGLIVAFFTNMGERFGFYTMMAILVLFLQVKYGLSEKA
                     AGDYYSWFYFAIYALSLIGGILADWKKQYKTVILYGQIIMFVGYVLMAMPGMSLYGTL
                     GALLIISLGNGFFKGNLQAVVGQLYDDEKYAKYRDSAFMVFYMGINIGAFFAPFVATG
                     IRNWWLETNGFIHDGSLPALCHQMLNGTLKNTAEFQSLANNAVLSKVPVTDLSAFAHQ
                     YLDVFSRGYNWAFAIAAVAMVVSLIVYVIFNKLLPNKSIAVAVEKQTNTIDNKSNHTG
                     RILFVSLFLMAITTILVYIIPVLNSPSEATSKIGLGLALGLFVAFVSYIFQMSTKEER
                     PRVISLILVFIVVIFFWMSFHQNGLTLTLFARDYTVKEVGPFTNLFFNLESILCVIAT
                     IAGIVLMLRSKSKMKDRVIGGVLTALFGVACYYFINHAADKNPISPEIFQAFNPLFIV
                     SLTPVVMGFFSFLSSRGKEPSTPRKIGFGMIIAASAFIIVLVGSLNLVSPYLLNGAPT
                     PETSRISPYWLMSSYLILTIAELFLSPMGLSFVSKVAPPRFQGLMQGGWLLATAVGNK
                     LLFVGSTLWGRVDLYVLWGVFFICCIISATFIFAMMKRLEKVTK"
     gene            complement(16437..16994)
                     /locus_tag="PKH58_02935"
     CDS             complement(16437..16994)
                     /locus_tag="PKH58_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88013.1"
                     /translation="MNNNEFQEKSQTTTTVNKIPKKPNRFVKYILDHKLVFSLIFALI
                     VVYFWGQWRYSKLEKQQKILIESYNRKIDSVQLSGMLLTSKAFSWAVRTDLLRNNPDQ
                     IQLYLNNIVREPQITKAYVVDTKKSIILFSSNRAEAGTAFLDVVAMQTKETFYQQRDS
                     IVRFISPFMELNSKAGITVIETSFK"
     gene            complement(17053..18504)
                     /locus_tag="PKH58_02940"
     CDS             complement(17053..18504)
                     /locus_tag="PKH58_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNX88014.1"
                     /translation="MKANFENRRGSGASGASRDGNSYEKRDVKSVLKSRGNNFHDNDE
                     RGDKRHNPNELRSENSGRPRFTVKKSGDQDDNTRFGERKSSDSNRYHSNSDRKPLGNS
                     NFRNSDDNRRPFNSDRKPFANRENRGGDDRRPYNSDRKPFGNRDERNNDRPYNNDRPR
                     FVKKGEQGDRPAFKKRYDDDQPQQGKLRLRKAGNTGNSEFVETRFDNRSSGITFRKRN
                     KDANYDPNAKYSKKKQIEYKSSNVDPTKPVRLNKFLANAGICSRREADEYIQAGVISV
                     NGEIVSELGTKILPTDKVMFHNQPVRSERKIYILLNKPKDCVTTSEDSHAKLTVLDLV
                     KNACSERIYPVGRLDRNTTGVLLLTNDGDLASKLTHPKYNKKKIYHAVLDHDLAIADF
                     EKILEGIELEDFTIKADALEFVKEDSLKEVGIEIHTGQNRVVRRIFEKLGYKIVRLDR
                     VYFAGLTKKNVPRGKYRFLTEREVNMLKMGAHE"
     gene            18868..21009
                     /locus_tag="PKH58_02945"
     CDS             18868..21009
                     /locus_tag="PKH58_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HNX88015.1"
                     /translation="MKKLIFIFMLFIATLSGYAQMLMSPDGNLTMNFSLSSAGEPLYQ
                     LSYKGKEVIKPSKLGLKLKDTTWLTSGFTLKDNKLSSFNETWKPVWGEVSQISNHYNE
                     MLVTLEQTTQKRFMKIRFRLFNDGLGFRYEFDRQPELSYFQVLDECTEFALTGDHKAY
                     WIPGDYDTNEYFYTTSKLSEINADAVNGSGIGTHGYFAKNAVQSPLMMKTADGLYINI
                     FEAALLDYSAMHLLVDQENFVLTASLVPDAYGTKVYMQTPHNSPWRTVIVSDKAADIL
                     ASKTILNLNEPCKIENTDFIKPQKFVGIWWGMHVPDYWTWNYSDTPNLKLKNTDWNAL
                     KPNGRHGATTENVKRYIDFAAANGISGVLVEGWNIGWEDWSGNWKEEVFDFITPYPDF
                     DVKGLQNYAASKGVKLIMHHETSASASNYERRIDKAIEFMKQNGYDAVKTGYVGWIIP
                     RGEKHDGQWMVNHYQRVIEKMAANQIMIDQHEPVRPTGQCRTYPNLMACEAARGNEFN
                     AWSVGNPPEHETILPFTRLMGGPMDYTPGVFEIKMDHYKTGSTNQVHTTLAKQLALYV
                     TMYSPLQMAADLVENYEKKMDAFQFIKDVAVDWDDTRYLEAEPGDYITIARKAKGTGD
                     WFIGAITDENARVANIDFSYLEPKTWYLATIYGDAPTAHWKDNPMAYEIRKVLVQNTS
                     VLKQQLAAGGGVAISVKKAAKTDLKGLKKLK"
     gene            complement(21014..21373)
                     /locus_tag="PKH58_02950"
     CDS             complement(21014..21373)
                     /locus_tag="PKH58_02950"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNX88016.1"
                     /translation="MKTYTQQHLITEYESATAMGSGSLEVYATPAMIALMENTAVQAI
                     ENIETGFTTVGIEINVQHLRASKPGETVTCTAKLVKHEGKLYEFQIDVENAKGDKTGT
                     ATHKRVAVRIEKFMNSL"
     gene            complement(21391..22344)
                     /locus_tag="PKH58_02955"
     CDS             complement(21391..22344)
                     /locus_tag="PKH58_02955"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX88017.1"
                     /translation="MYLRSIKFNPAEKLTLYYIIISSFVAFYICMKFGTGVSFIGIRL
                     LISAVIFFLAYLGNSRKKSSINVIRYLFIASLFIYWYTETFYLNRYFENFDHLLARTD
                     FLIFGFQPSLMFAQVYPQHWFSELMNFGYISFFPLLIFTCLYFYHTDRKYAEFFFFTT
                     VLTFFLFYLLFLLIPASGPQYYFQLIDNKQISAGIYPSIVDYFSNNYICLRHTSPSGI
                     FQSCLDFIRGHAERPTGAFPSSHIGNSTLVIIMLLAKREYKLALILSPVYVLLVFSTV
                     YLGAHYVIDVIAGFISAFTFFGLSLYLYPLFSGRNHTDFKL"
     gene            complement(22512..24917)
                     /locus_tag="PKH58_02960"
     CDS             complement(22512..24917)
                     /locus_tag="PKH58_02960"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNX88018.1"
                     /translation="MKRAVMLILSSLFVSVSTFASEADIAIPDLHEGTFHIFGNQITA
                     WNFLFYGALVICFTLGISLWLHHEVKKQKAHISMLKVSETIYQTCKTYLLQQGKFLLM
                     LFAFVAVIMAYYFLALQGKSVGTLLFVLCFSILGMGGSYAVAWYGIRINNYANSRTAF
                     ASLRANPWDVVNIPLRSGMSVGLFLISLELVMMVVILLFVPREIVGICFLGFAIGESL
                     GASVLRIAGGIFTKIADIGSDLMKVVFKVKEDDPRNPGVIADCTGDNAGDSVGPTADG
                     FETYGVTGVALITFITLAVHNPDIQAKLIVWIFGMRFLMDLCSGISYFINQAISKRKY
                     KGMKIFDFEEPLMRLIWIAAILSISTSFFMSHLLVGDLPDSTLWWKLAGIISCGTLAA
                     VLIPEFTKFFTSSKSAHVNEIVTASREGGPSLNILSGIVSGNFGAFWTGLLIVGLMTG
                     AYFISITGNLEAVLGPHATIFAFGLVAFGFLCMGPVTIAVDSYGPVTDNAQSIFELSQ
                     IEQIDGISEEIEKDYGFKPDFEMGKHYLEANDGAGNTFKATAKPVLIGTAVVGATTMI
                     FSIILLLESVGMLSVSLTSAPILLGLICGGAVIFWFSGASMQAVTTGAYRAVEYIKRT
                     FKLDKTEANTEDSKEVVKICTQYAQNGMWNIFIALICFTLGFAFFDSNFFVAYLISIA
                     CFGLFQAIYMANAGGAWDNAKKVIEVDLKEKNTPLHEAAVIGDTVGDPFKDTSSVSLN
                     PIIKFSTLFGLLATEISLKMKLDALKGIGTDYSGYIGVIFLVIALIFVWRSFYKMRIP
                     LKK"
     gene            25492..28851
                     /locus_tag="PKH58_02965"
     CDS             25492..28851
                     /locus_tag="PKH58_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNX88019.1"
                     /translation="MKTKLFVLSILLMSFTALNAQVTTSSMSGLVTDAEGAVVGAAVV
                     ATHTPSGTTYRTITNVEGRYNLNGMRVGGPYSVEITYVGYGKSTTNNITLSLGENYAL
                     NVVLSEETSTLDEVVVTAIRTKFTTEKTGAVTNITNTQIANLPTVSRSIIDVTRLSPY
                     GGNGMSFAGTDGRTANFTVDGANFNNNFGLSDKLPGGGNPISIEAIEELQVVIAPYDV
                     RQTNFIGGGVNAITRSGTNTFKGSAYAFHRNENLRGDAIRGKQITGARDKDRNTTYGF
                     TLGGPIIKNKLFFFVNGEMAKTPTIANRWRPSTNGVADANNYISRTTVSDLQTVSDYV
                     KSKYGYETGSYTDFPADESNYKLLARLDWNITNQHRLALRYNYTKNLSWVAPNATSMD
                     GGDRMTEARLSQASMSFANSMYSMSNKVHSVSLDLNSRLSDNLSNQFLATFSKLDDVR
                     GSNSDEFPFIDILKLKEDPTGWDKPVQQSYMSLGYELFTWNNAVHNNVWNIKDELTYY
                     IGNHKIVGGIAYEYKMADNAYMRNGTGYYRYSSLDDFLNEAAPEIVDLTYGYDGESNP
                     AARVRTSKLGIYGQDDWSVNDKFKLSYGLRIDGLFFNNNDLITNNAILALDYSGRNID
                     TGKWPATNIIFSPRIGFVWDAFGDKSLKVRGGTGLFSGNLPLVFFTNMPTNGGMVQYQ
                     AQISGKTKVFDAKKGEIVKGYTGTYTTDKDGNRYVDMSQFAGGLVKDANGKATTAALY
                     DKLISLGYPATISPEDGTVPSAIAAVDPNFKMPEVWKTSLAVDYAFKTSFPFSVSVEG
                     IFNKTINGVSISDWSIPSVGGFARFNGVDNRPVYSAGYRVGTKAFVLENTSRGYGWSG
                     NITFNAQPTKWLSMMAAYTHTVAKDVTGMPGSNAESAFTYVPTVEGPNHIKLHNSQYN
                     TPDRLVASLTANDKSGNHYSLIYEGWLGGANYSYMTANDINGDGYNYDAIYVPTDDEV
                     ANNQFRFVSESDKTRFMDYVHANDYLKNQQGKYAEAYSVYSPWVHRIDFGYKHDFALN
                     SGKNKQMLQLSLDVKNVMNLFNSSWGVAKYLNPEIGSEARVLKYEGVDADGFATFSTP
                     SSIKGNTQTFTPSYSLGQCWYASIGIKYMFN"
BASE COUNT         8407 a         5815 c         5758 g         8887 t
ORIGIN      
        1 aggcggatcg tcagacaagg acgcttttta accgcttcac tccggcaatt ttctttacgc
       61 tttttctcta aggcggaaag agggagcgag accaaaattg agctttaaat ccaatttaat
      121 gtatattgtt cagattttca ataacatata cccgcttgaa ttactaatat cggacgccaa
      181 tactattcag catttatttg atgtcggtta cttattatca agtgtgtttc agacattcat
      241 ttgaaaatct taactccgaa gaaagttgca gtttacttca tttacaactc tatagctcct
      301 cctttagggg gctggggggc tggtagtttc attcacaatc aaatacatca gcgatgtata
      361 gggcggaatt tcttcgttgc cattcatgcc gtatccgaga taccagggta caataagctg
      421 catagtttca cctttgcgca tcaataacag tccttcttcc aatcccgtgg gaatttcctt
      481 tttacccgga cgaatggttc ttgttccgct gttgatttct tcacccgcca gattgtatgt
      541 ggtgaagctg taactgatta tcgaatcatt cttcagcgag tctgtccggg tggagtgtaa
      601 cacgttgtac caaatacctg attttgattt gctgaaatgc atatcgttcc gggcgatcat
      661 agcctggata atgctgtctt cccgtaaagc aagaatttta ttcatttcaa gcagagaatt
      721 tccggttgta tctgctgcca gaactttatt ggatggcaac tgaggtgcct gcttcctgca
      781 tgaacttatt ccggtaatag caacgataag taatgctgcg aacagaaata ctgttgattt
      841 cttcattatc ggatgtttgc aatgctgatg atatcagcga aaacaccggc agccgtcacg
      901 cttgctccgg caccgtaacc tttgattatc atcggaaatt cgttgtagcg ttcggttgta
      961 atgagaatga tgttgttgct gccctgaaga tcgtagaacg gatgcatgct tccgacggtt
     1021 tgcaatccca cgctgcattt gcctttatcc atagtagcaa caaagcgcca atgcaatccc
     1081 tttttctcca gttctttacg tttctcctcg aaatcggcat ccagttcggg aatcatcttc
     1141 cagaaatcat cctgacttcc caggaaataa ggatcgggga tgaagagatt tttctgcacg
     1201 tcttcctgat ttattttgta accggcttcg cgggcaagaa ttaccagttt gcggataaca
     1261 tcctttccac tgaggtcaat tcttgggtca ggctccgaat agcgggcttc cttggccatg
     1321 cggattgcct tgctcaatgg tatttcgctg ctgatggtat tgaaaataaa gttaagcgta
     1381 ccggaaagaa ccgcttccat tttaagaatg gtatcaccgc tgtttgtcag gttattgatg
     1441 gtgttaatga taggaagtcc tgcacccaca ttggtttcga acaggaattt aaccccacgc
     1501 tgacgggatg tttctttcag ttgcacgtat ttttcgtact ccgatgaagc tgcaattttg
     1561 tttgccgtca ctaccgagat gttattttca agcagtttct gataaatatt agctacctcg
     1621 gcactggcgg tacaatccac aaaaacagaa ttgaaaatat tcattttaag aatctcatcg
     1681 catatcagct gggtattggc agcttgtcct tcctttttca gtctttcaac acaattttcc
     1741 agcggcactc cttcgcgtgt gaaaatcatt ttcttttcgg aagcaacccc aacgacgttt
     1801 attttcagcc cgttatatac catcaaattc ttttgttgtt tttttatctg gttcagcagg
     1861 ttgccaccca cggttcccac tccgataatg aaaagattga gcacctgata ttcggacagg
     1921 aaaaacgagt cgtgaataac attgagcgcc ttacggagta cggctctgtc ggtgacaaac
     1981 gaaatatttg tttcggaagc tccctgggcg caggcgataa cgtttatacc gtttttacca
     2041 agcgtaccaa aaagctttcc ggcaataccc ggcgtacgtt tcatattgtc tcccacgatg
     2101 gcgatggtaa cgaggtcgtc ttctgagaat atctggttga tttctccctg ctgaatttcc
     2161 ttagcaaatt cgttgcgaag cacttcgact gccagctgac tatcggcatt ccgcacaccg
     2221 attgaagtgg tattttcgga agatgcctgc gatacaagaa acacgctgat gccatttttg
     2281 gcaagtatgc ggaatatccg gtaattcacc ccgataacgc caaccatacc caatccttga
     2341 atggtgataa gcgaggtatc gttgattgaa gaaattcctt tgatggcctt ttgctgattt
     2401 ttcagttgcg aacttttttc gctcgaaatg aaggtgccgg atgcttcggg gttgaaggtg
     2461 ttttttatct ctaccggaat gtttttgtga tatacaggga aaatagttgg cggataaatg
     2521 accttcgctc cgaaattaca tagttcggtt gcttcgctga aactaagcct ttcgatggtg
     2581 taggcattgc tgatgatttt cggatcggca gtcataaaac catctacatc tgtccatatt
     2641 tcgagttttg ttgctttcag cgccgatgca aggatggctg ccgtataatc agagcctccg
     2701 cgtcccaggt tggtaatgta accggtttta gcatcggacg atataaatcc gccgcatacg
     2761 caaacatcat gtgtttcggc aaaaatatcc tgtatgagtt gattgctgat ggtgaaatca
     2821 acattgtgct tgtcgaactg attgttggtt ttgataaaat tgcgtgcatc atagtgaact
     2881 gcccctttta tcagattact aacaatgatg gaagaaataa cctccccata gctcacaatg
     2941 gtatcggtga tacggactga gatatcctta atcaggtaaa tgccgcgaag gatgttggaa
     3001 aggtcggtga aattatcctg cacaagtttg atggtttgca gttttttctc gggtaaaatg
     3061 acattttcaa tcagttccag atgtctcgaa accatatttt catattcggt cagataagct
     3121 ttattacctt cggaagccat ttttgaggtg gcgtaaagca gatcggtgat gcccgataat
     3181 gctgatacta ccactatcac agcttcctgc tgcgactcta caattttctt taagcttaaa
     3241 ataccttcga ctgagccgac tgatgttcct ccaaatttga taactttcat cctgagaatt
     3301 ttaatttgag tattgatatc gctataaatc ttcaatactg ataatagaaa ttgacaaaca
     3361 atcttttact gtaatttttc cgattgtgcg gaaaaaaaag aaatatgata ataaaaagcg
     3421 gttttctgaa ttaaaatccg aaatatattg tcggtaaagt gtcaaaccgc tttcagtttg
     3481 caatattact cattttttgg caaatatgaa tgatttgttt tatttttaag gtaaagtatt
     3541 gcaaagttgg gaaaattggg ttacttttgc accacttttg taaatggtct cgtagctcag
     3601 ctggatagag caacagcctt ctaagctgtg ggtcttgagt tcgaatctca acgagatcac
     3661 aaaaaaaact gctaattgtt agcagttttt ttgttttata atatacgtac gtttcagatg
     3721 ctgagtatat tgtatttgtt tataatcagc tgttgagctt tttctatttc gtctttggtg
     3781 ggatatttaa tactgaaatc acgtatttca cccttctcat tttcaactga ataattgaaa
     3841 tgttcgctgt ccgacgactc catcagtttc cattctccct tatcggtatg ctgatattcg
     3901 cagaaataaa acaacacttc attgcccagc atttcaaatg ctacgcggac ctttgcatta
     3961 tcgggccttg tgtactccac atagtcaaca aaatctttca tatacttcgg ataaaatttg
     4021 gattattaaa agtaaaacag ttgaacagat aaatagtttt gcctgtatga ctattggtgc
     4081 cagtcgagta ttcgtctctc gccttccaaa gcctgaattt cagatatttc ctgagtgact
     4141 tcgatgacgc ccttgtattc atttctttca tttcttacgg caaaatactg aattaatatc
     4201 ttttttccct tcatattaat ccagaaatca gctttgtctt ttgtgccatt gcggaatgat
     4261 tccacaatct gttcaaccac atgaacactt tcgtgcggat ggcagttgcg tacgtcgcgc
     4321 cccagtatag cgtttgtgcg ggtaaaaatt ctctttttgg gagttgaata aaagcatact
     4381 ttattgttct catcgacata ggtaatatcg accggtaaat ggctgaaaat aagtatcaac
     4441 tgctcagccg acaaagctcc gcttttgaga tcaaccagtc cggatgaaac gttttttact
     4501 gatggttcag cgattttctt ttgcggatta aagtatggaa agccaatttc aacactttcg
     4561 tcccacaatt tttcaatttc tgaggcaggt acgtttttgg cgataaacgg ataaagcaat
     4621 cgttcttcgc gaaaggcaat ggaatagatg ttgtaaaaca cctcaccggt aagccggttc
     4681 agtttttgaa gatccaattg acctgaagcc aacaaatctg tcagatcgtt caggttacgc
     4741 cggatatcat cgtgtatcga ccacatcacg gcaatacaac gatattcgaa aaggtatttt
     4801 tccacaagcg gaaacagcac attttccttg atgacataat actgagtgaa ttcctgaact
     4861 tccttccaga gacggagcat ttcggtcagg atatgttcag taacaccctg tttgttgatt
     4921 tgcaggataa aaggcctgag tttgttcagt ctgttcttaa cctcattgtt gttacgaatg
     4981 cagatatcaa aaaacgtatc gtaagcgggg gctgtgtatg gatggctgtc gagtgagcgg
     5041 cgcaatacat taagcagctt gttgatgccc gattttatat cctccatcgg atagctgctt
     5101 tccatcagct tatgtaccag tattaccaca tcatcgggta tcgtattgtc gatcgtatgc
     5161 tggtacttct ttattgcttc tgccggttgt tttttgtagg tgattgcatc aaacaaaatg
     5221 ttcaggtcgg ccaaacgatt aatctgatgt gtgctaaatt cggacatggt atatgatctg
     5281 tttaagatat ttctactgct aatttatgta atcagaatat ctgttttgag atttgtgttt
     5341 gtatcaaatt aaacccctgt tcttcagtat ctccgacatt tcaacctgca attttctggc
     5401 ttcgtcatgt gccgcttctt taaagtcgga ttcagatgat gcatagatta tctgtcggga
     5461 agaattgacc agtaatccac actgattgtt caaaccatgt tctgcaactt ctgccagact
     5521 tccaccctga gctcccacac cgggaaccaa cagaaaatgg tcaggtacaa ttgatcggat
     5581 ttcccgaagc atttctgctt ttgtagcgcc caccacgtac atcatatttt cagcgtttcc
     5641 ccatgattgt gattgtttca gcactttttc aaataaacgg tctgtgctgt cggcatcctt
     5701 catcaactga aagtcaaatg cgcctttgtt ggaggttagt gctaacaata ttacccattt
     5761 gttttcgtaa gtcagaaacg gtgttacaga atcttcgccc atatatggag caactgttac
     5821 ggcgtcgaaa tccatatttc cgaaaaaaga tcgggcatac atagccgacg tatttccaat
     5881 atcgccacgt tttgcatctg caataataaa ctgatcgggg tattctgccc gaatgaaatc
     5941 gacagtacgt tccaacgcat cccagcctgc aagtccgatg ctttcgtaga atgcgagatt
     6001 gggtttataa gctacacaca aatctgctgt cgctttgaca atctggacgt taaagtcaaa
     6061 aatcggatcg tcagaattat tcagaatgtg ttcgggaatt ttatttatgt cggtatcgag
     6121 tcccacgcaa agaaatgatt ccttacgttt tatgttttcg aaaagttctt gcttagtcat
     6181 tttagtgttg tgttatgtta ttttttttgt aagtttccag attttccatc agcagatttt
     6241 ccagtcgctt gtatttgata tccaataaat tgggattaat tccgaccgga gcattataaa
     6301 gagtttgatt gatttgcata tttttccgta cattcgtttt tgtctgtcgt tcaatcaatg
     6361 ccccgggatt aaaaagatgt atatttatag ttcgtatacc attggcttcg tacaatgaaa
     6421 atctttcaat ttcattccaa ctgataaaag tatcatcagg attattccgg tcggtaacaa
     6481 taccactctc gttgagtatt atgcctccgc cctttttcaa ccggtaaaca atataaacgt
     6541 caagtatcgt gaataaggtt agtcctgcaa atccggtgat actattccgg ttactgtcaa
     6601 aaatcaacca gatacaaata agcgtaaacg ctaaagaaat aatcagaagc gtaacaggtt
     6661 ttactttatt gtatttgatg acagtttcca tttatgccag ttcactttct ttcattcgtt
     6721 ctgcgttttc agcaatgata agctggtcaa ttacatcctg aatatcacca ttcatgaatg
     6781 cagtcaggtt atagattgta taattgatgc ggtgatcggt gattcgtccc tgcggatagt
     6841 tataggtgcg gatttttgcc gaccggtcac cggtagatac cattgtttta cgtttggaag
     6901 ctatcgcgtc tatgtgtttc tgatattcca tgttgtagat tttagtgcga agcatttcca
     6961 tggctatttc acggttgcct agctgtgttc gtgcctgctg acaaaccacc acaatgccgg
     7021 tagggcggtg agtgagttga acttttgttt ccactttatt tacattctgt ccaccggctc
     7081 cccctgaacg cgaggtatga aaatcaatat ctgccgggtt aatgtctact tcaaattcgt
     7141 cggcttcggg caatacagct accgtcgatg ccgaagtgtg aacacgtccc tgtgtttcgg
     7201 taaccggtac gcgctgaacg cggtgtacac ccgattcgta tttcagtgtt ccgtaaacgt
     7261 tttctccggt tacagtaaaa atgatttctt taaatccccc aattgtacct tcactgaagt
     7321 cggttacttc cactttccag cctttatttt cgcagtagcg tgtgtacatt ttgaataaat
     7381 cgccggcaaa aatagcagct tcatctccgc ctgtaccacc gcggatttcc attactacgt
     7441 ttttattgtc ttcaggatcg gcaggaatga gcagcagttt gatattttcc tccataacag
     7501 ggatttgagg ttccagatta tcgagttcag cttttgccat ttcacgcagt tcggtgtcag
     7561 attctttatc gtaaatttct ttggcttcag ttatgttagc caatgctgtt ttatattcct
     7621 ttacagcatt cattattttg tcaagttcat tgtattcctt attcagcttg atgtaccgct
     7681 tcatatctgc aatcactgca gggtcggtta tcagcatgga tatttcttcg tatttgtgtt
     7741 gaagtccgtc taatttttgt aataaagaat tttctgccat ataattaatt taattttacg
     7801 ttgtatgaga gtataattgg ttttaataaa ttgtctgtga gtaagtgttt ttttcagctt
     7861 ccttttgcat gttttcggtg aatttgtcaa tacgccattc aacgatactt ttataacggt
     7921 tctgagtgat gaaaaacttc aaaagccatc caaactgaat atgagtttct gtttgaagta
     7981 actgattgcc gtttttcatg tgacttgatg tgatggtagc atgagctttt ttcagcaaga
     8041 atcccgaatt aacaacaaca accgatccta tcagttgtcc atttggtagt aattcggttt
     8101 ttacgacagc atctaccttt acatccctaa ttgtctgaaa gttgggaatt attacgtcaa
     8161 ccagtccatg ggtgattccg gtatttttat caaacagcga ttttttcaaa acgataatca
     8221 tttcatcgtt tttctgaccc tgaagtccca gatctttgag tgcccagtca aagagcttca
     8281 tcggtttgtt attcagttgc tcaatcattt gcaaaatcac tgcatttgac tgcaatacat
     8341 ttccgtttac ctcttttcgg cacacagtta caaattcacc atcagtatat gctgttttca
     8401 ggtactgata gggcgtattt gcctgtactg caatgaaggc taatgaaaca aacagaaatg
     8461 taatgatttt tttttctata tttttcatca aaaaagccaa agggaaaaat gatcctttgt
     8521 acttagttag ttataacaaa ctgaaagtca tttttattca aaaatttgta aattatattt
     8581 ttattttttt tctgtcaata cctatatcgt ttagagatgc agggtcttcg atgggttcaa
     8641 tgtgcgttga gcaggttgcc ggaagttcaa acaggttttc aatgtactct tcaatatatt
     8701 cagatttgtc atgtcctttc tgaaccgacc atgaaccagg tacaagcaaa tgcaatgaga
     8761 taaaaatacg ctgtccggcc tgacgggtca tcagtgaatg atattcgata ccctgttctt
     8821 tttgggattc cagaaaattc gttatcagct tttgtttgtc gggagaaata accgaatcca
     8881 tcaatccgtt tccggaacgg tgaagcaggt gtacacctgt ccagatgatg tttaatgcaa
     8941 caataactgc tattatcggg tctaaaatgt ttagtccggt aagtttgacc agtccgattc
     9001 ccaccaatac acccacagaa gtccacacat cagtcatcag atgtttgccg tcagcttcca
     9061 gtaaaagaga atgtcttttt ttgccgtttt ttatcaggat caaagctaca gccagattaa
     9121 tggctgatgc accggttgaa ataagtaaac caactccgac gttttcgaga ggaagcggat
     9181 gaatgatacg cggtattgca gaccagataa tactgaatgc ggcaaccaga attagcgctc
     9241 cttcaatggc gcttgaaaaa tattcggctt ttgtatgccc aaattcatga tctttatcag
     9301 ccggtctttc agaaatgctg atcattatca gtgctatgac tgccgcaagc agattaacgc
     9361 aggattctaa agcatcagaa aacaagccca tagaaccggt aagaaaataa gctccgaatt
     9421 ttaatgcaat tgttagtact gctgctgcaa ttgacaggta agcaaacttt tttaaagatt
     9481 ctttagtcat aatcctgata tgaaatgatt tattttattg taaatcaaaa aactaaaatc
     9541 atcctgtgtt gaaaaaaata taatgagtta attttcagat tgataattta tttttttcct
     9601 tccacccatt tgcgggcatt aacaaacgct tccatccacg gtgtaacttc atcgttttga
     9661 actcgctcag ttggataatt ggcacattgc caggggaata tcgcacgttc ggggtgaggc
     9721 atcatagcca gatgacgacc atcggccgaa caaatacctg caaccgagaa atcggaaccg
     9781 tttggattgc cgggatattc gtcatagttg tacttagcga tgatattgta tgcattttca
     9841 cccattggaa gtgagaattt tccttctcca tgagctaccc aggcgcctat tttgcagcca
     9901 gaaagcgagc ctaaaattac cgaattgttt tccgggatgg taagtgaaac aaattccgat
     9961 tcaaatttat gagaatcgtt atgcagcatc ttagccctgt tggtatgctc aggattaatc
    10021 aggtttaatt ccaccatcaa ctgacagccg ttgcatatac ccagacttaa cgtgtcattt
    10081 cgtttgtaga agttgtccaa agcaactttt gctttctcgt tgtagaggaa accacccgcc
    10141 cagcctttgg ccgatccgag aacatccgaa ttagaaaaac ctccgcagaa aacaatgaaa
    10201 ttgatatctt ccagcgtttc acgtcctgac gcaagatctg tcatgtgaac atctttgaca
    10261 tcaaaacctg ccagataaag tgcataagcc atttcgcgtt cgccattcgt gcccttttcg
    10321 cggatgatgg ctgctttcac tcccgatggt ttacggtcgg gtgaaagtcc cagttgcgat
    10381 aatttacctg taaatgtttg agggaaatgg aattcaagct tttgattttt ataattttca
    10441 aatcgtgctt ttgccgcttt ttctccgctt tgacgccggt cgagcaggta agaagcttcg
    10501 aaccacatat cgcgcaaatc attgatagca aaagaataag atgtcttttt cttatgaatt
    10561 tcaagcctgc gttcagctat gggttttgca atctgagcaa atccaattcc gttgtttttc
    10621 agaatttttt ccacttcagc tttgtttgca gtctggataa gtacgccagg attctcggca
    10681 aaaaggattt taaccagcga tgtttcagac agatcatcca gtttgacttt caaacctccg
    10741 attgaattag caaaacacat ttcgagcaat gcggtaatca aaccaccttc cgagatgtcg
    10801 tgtccggcca gaataagatt tttattaatc agttcctgta ttgaatcgaa agctgctttg
    10861 aagtattcgg agtctgtaac agtaggcact tcattaccaa ttttatttaa tgtctgtgcc
    10921 aatacagaac ctccaagact gagtttgtcg aatgaaaaat caacataata cagagaggtt
    10981 tcgggattat tgacaatcac agggctgaca acccgacgaa tgtccgaaac ttcggctccg
    11041 gcagaaataa taactgttcc gggagagaaa actttttcat caccgtattt ttgagtcatg
    11101 gacagactgt ctttgccggt cggaatgttg ataccaagtg agcatgcaaa atcgctgcag
    11161 gcttccactg ccttgtatag acgggcatct tcacccggat ttttacaggg ccacatccag
    11221 ttagcactta atgaaacact gtccagtccg tctttcaaag gtgcccaaac aatgttggta
    11281 agagcttcgg caatggccag caccgatcct gctatgggat cagcaagtgc agccagcggt
    11341 gcatgcccga tggaagttgc aataccgctt tttccacgat agtcgagtgc tactacaccc
    11401 acatcgctca aaggcagttg tatttcaccc acacattgct gacgggcaac cttccctgta
    11461 accgacctgt ccaccttatt ggtaagccag tctttgcatg caaccgattc catttgcaga
    11521 acattctgaa cataaccaag caactgttct tcgtcaactt ctacggatgc aaaattttct
    11581 tccagactat tatcggtaat gacggtgcgc ggcggtttac caatcatata ttccagcaat
    11641 aaatcaattg gtttctgacc atctttttgt tcaaatacga attgcatatc acctgttgtt
    11701 tcgcccacta catagaatgg agcacgttca cgttctgcaa tctgttgtac ccgttcaata
    11761 tctttttcct gaagtaacag acccatccgc tcctgacttt cgttgccgat aatttctttg
    11821 gcgctgagcg tgggatcacc taccggcaaa ttatccattt caatcttacc gccggtagtt
    11881 tctaccagtt cggagaggca gttgagatgg cctccggctc cgtgatcgtg aatcgaaacg
    11941 attggattgt cgtcagattc tgccagggca cgaataacgt ttgcaacccg cttctgcatt
    12001 tcgggattgg cacgctgtac cgcattaagt tctatcgcat tatcatattg acctgtttca
    12061 actgacgaaa cagcaccacc acccatacct atccggtaat tgtcgccacc catcacaacc
    12121 actttttcac cggtagctgg ttcgcctttg agagcatccc ggcgtgaagc ataacctaca
    12181 ccaccggcaa gcatgattac cttgtcgtat ccgtatgtct tgtcgttttc gttgtgttcg
    12241 aacgtaagca aagaaccgca aataagtggt tgcccgaatt tattaccaaa atcggacgca
    12301 ccgtttgatg ctttgatcag aatttgttcg ggtgtctgat aaagccattt ccgttcttta
    12361 agtgatttct cccacggctt acctgcattg cggggatatg aagtcatata cacagccgta
    12421 cctgctatcg gcaagctagc ttttcctccg ccaagcctgt cgcggatttc accgccggta
    12481 ccggttgcag cgccgttgaa tggttctacg gttgttggga aattatgtgt ttcagctttg
    12541 agagaaatta ccgattcgat attttttgtt tcaaagaagt ccggcttttc gtccgattta
    12601 ggcgaaaact gttcgataac cggtccctga ttaaatgcta cattgtcctt atatgcagaa
    12661 acaagatcat tcggattatc ttcggatgtt tttttgatga gtttgaagag tgataattct
    12721 ttttcttctc catctataac aaactgacca ttgaatattt tatggcggca atgttctgaa
    12781 tttacctgcg aaaatccaaa tacttcgctg tcggtgagtt tccttcctat tttctcggaa
    12841 atgctgttca ggtattctat ttcatcttcg ctcagtgcca gtccttcctg ctggttatat
    12901 aatgcaatgt catcaatgtg ccggattgct tccggctgtt tgttaatcgt gaatatctcc
    12961 tgattaagct ctgtgtaaat tctttgcagc atgggatcgt gatcatcttt atcaagtacc
    13021 ggaaagaatt cttccattct taatattccc ctgattgcca tattctgtgt gatttctacg
    13081 gcattggtac tccagggagt aatcatttca cgtctgggac cgatgaattt tccggttatt
    13141 ttatcttcgc tgagtagttc tgcttcaccg aataaccata ttaacttgtt gatatcgtca
    13201 tcggataggt tcaatgactg tactgcatag attttatttt cgggcgtgcg gaaaaaaagg
    13261 atcatggcgt gaatgtatta atgtggattt ataaattatt ttagtgacaa catgacgtta
    13321 aggcattgga tattaagcgt ctaaaactga attgaacatt ttcaattact ttcgcaaaga
    13381 taataatttt taacggtgcg gggaatagat aaaagtcctt ttttcagttt aatttcagct
    13441 ttttactcag gaatatgaat tatggatttc aaagaataat ttttcctaac gaaaaaccaa
    13501 aaaatgataa aggcagacat gcaagcaaag tccgcctttt agtatgtttg aaaagttgaa
    13561 tttaattact gcttatcttt tttttagaac tgaatagagc caaagtatca atacagctcc
    13621 tcctgttgat actatcaggg ttttaatact gaactcattc acagtgccaa gtccaaagaa
    13681 tgaacctatc cagcctccaa cgattgctcc aataattcca atgataacag taattatcca
    13741 tccgccggga tcagggccgg tatgaatggc ttttgccagt gcaccggcta tcagacctaa
    13801 taaaatccaa gaaattattc ccataattta tgctgattaa atatttttga actataacga
    13861 aaactttttg aaaattgttc atgtatatgc tgtagtaatg tgcaggaaag atgtttcttg
    13921 atcgaaaaac taactgtttt ccatgaaacc tttagctttt tctttagtaa gttctttctt
    13981 gagtcggatt attacttttg gattttcggc agcgatattc tttttttctg caatatcagc
    14041 tttaaggtta tatagctggt cgagctgtga atttacgagt tctgtttcag tttaatttta
    14101 ttggacactg tggtagtgtt ttatttacag cggatttttc agacgaattg aatcgtcgta
    14161 ctgtacctgt aaattccaaa gttccttttt aagtatactg attttattgg catattctgg
    14221 attatcaatc aggttatcaa taacaacaaa taaaaatcct gcaataatca ttgcaggatt
    14281 ttttaaactt ataacttgtc tgtcagtata ttcgtaactt gtaacagaat acttattttg
    14341 ttactttctc aagccgtttc atcatggcaa aaataaatgt tgccgaaatg atacagcaga
    14401 taaagaatac tccccagagt acatacagat caacccgacc ccagagtgtg cttccaacaa
    14461 agagcaattt attgcctaca gctgttgcaa gcaaccatcc tccctgcata agaccctgga
    14521 aacggggtgg agcaaccttc gaaacgaacg ataatcccat cggactgagg aaaagttcgg
    14581 caatggttaa aatcaggtag ctgctcatca gccagtatgg tgaaatacgc gatgtttcag
    14641 gggtaggcgc tccgttgagc aaatatggtg aaaccaggtt taatgaacct accaaaacaa
    14701 taatgaacgc cgaagcagct atgatcatac caaaaccgat ctttcgtgga gtggaaggct
    14761 ctttgcctct ggaactcaga aagctgaaga aacccattac gaccggtgta agagaaacga
    14821 tgaacaacgg attaaatgcc tggaaaattt cgggtgaaat tggatttttg tcggcagcat
    14881 gattgatgaa ataataacag gcaacgccaa acaaggctgt gagcacaccc ccgattaccc
    14941 gatctttcat ttttgatttg ctacgaagca tcagtacgat acctgctatg gttgcgataa
    15001 cgcagagtat cgattccaga ttaaagaaca aattggtgaa aggaccaact tctttcactg
    15061 tataatcgcg ggcgaataat gtcagcgtca gcccgttctg atggaacgac atccaaaaga
    15121 atattacaac gataaatacc agaatgagtg aaataactct tggacgttcc tctttggttg
    15181 acatctggaa aatataggaa acaaatgcca caaacaaacc taatgcaagt cctaagccga
    15241 ttttcgacgt agcttcactg ggcgagttga gcacaggaat aatataaacg agaatggtgg
    15301 taatagccat cagaaacaaa gatacaaaca gaattcggcc ggtatggttt gatttgttgt
    15361 caatggtgtt ggtttgtttt tccactgcca cagcaatcga tttgttcggc agcagcttat
    15421 tgaaaatcac ataaacaata agtgaaacta ccatagctac tgctgcaatg gcaaatgccc
    15481 agttgtaacc acgggagaat acatccaggt attgatgtgc aaacgcgctc agatcagtaa
    15541 cgggtacttt actcaatacg gcattatttg caagcgattg aaattctgca gtatttttca
    15601 acgtaccgtt cagcatttga tggcatagtg caggcaggct gccgtcatga atgaaaccat
    15661 tggtttcgag ccaccagttt cggatacctg tagccacaaa cggggcaaag aacgcgccga
    15721 tgttgatacc catatagaaa accataaatg cagagtcgcg atacttggcg tatttttcgt
    15781 catcgtataa ttgtccaacc actgcctgaa gattaccctt gaaaaaaccg ttaccaagag
    15841 aaataataag taatgctccc agtgtgccat agagcgacat tcccggcata gccatcaata
    15901 cgtaaccaac gaacatgata atctgaccgt agagaataac tgttttatac tgttttttcc
    15961 agtcggccag gatacccccg atgagtgaaa gtgcataaat tgcaaaataa aaccagctgt
    16021 aataatcacc ggcagctttt tcgcttaagc catacttaac ctgtaagaat agcaccagta
    16081 tcgccatcat ggtatagaag ccgaaacgtt cgcccatgtt ggtgaaaaat gctacaatta
    16141 atccttttgg atggttcttc aacatattaa ttttttaatt tttttagtta atacttattt
    16201 cacaaatatt taatcggatt gcaaatataa tcataaagat ttaattatta aatttcctca
    16261 tgaagtttta ctgtgggtat aatagctcag aaaatgtctt tatttacgga atgaaaacga
    16321 cgggcaaaat ctgaaattca gatatttata attgatgcga accaattaat tccggttctg
    16381 tcaataaaaa aagggaacaa gtaagttccc tttaatatat tgttgttgca gatattttac
    16441 ttaaaactgg tttcaatgac tgtaataccg gctttactgt ttaattccat aaacggagaa
    16501 ataaacctta caatgctgtc tctttgctga tagaaggttt cttttgtctg cattgccacg
    16561 acatccagaa atgctgtacc ggcttcagct ctgttgctcg aaaacaggat tatactcttt
    16621 tttgtatcaa caacataagc tttggttatt tgcggttcac gaacaatgtt attcagatac
    16681 agctgtatct gatcgggatt attcctaagc aaatctgtac gcacagccca tgaaaaagct
    16741 tttgaggtaa gcaacatgcc ggacagttgg acactatcaa ttttccgatt atagctctcg
    16801 ataagtatct tttgttgctt ctcgagctta ctgtatcgcc actgtcccca gaaataaact
    16861 acaatcaagg caaaaatcag agaaaaaacc aatttatgat cgagaatgta ctttacaaac
    16921 cggtttggtt ttttcgggat tttgtttaca gttgttgtcg tttgcgactt ttcctgaaat
    16981 tcattgttgt tcataatcag tcgatggtaa taggaaattt caaggcatgt taatccgttt
    17041 ttaccttccg gattattcgt gtgcccccat tttaagcata ttaacttctc tttcggtcag
    17101 gaagcgatat tttcctcttg gaacgttttt cttggtaagc cctgcgaaat aaacacggtc
    17161 cagacgtaca atcttgtatc cgagtttctc gaaaatacgg cgtacaactc tgttctgacc
    17221 ggtgtgtatt tcaatgccca cttctttcag gctgtcttcc tttacgaatt cgagcgcatc
    17281 agctttgatt gtaaagtctt caagttctat gccttccaga attttttcaa aatcagctat
    17341 tgcaagatca tgatccagta ccgcgtgata aatcttcttc ttgttatatt tcggatgtgt
    17401 aagttttgaa gccagatcgc cgtcgttggt aagaaggagt actccggttg tgtttcggtc
    17461 gaggcgtcct accgggtaga tacgttcgct gcaagcattt ttcaccagat caagcactgt
    17521 aagtttggcg tgcgaatctt ccgaagtagt tacgcagtct tttggtttgt tgagtaaaat
    17581 gtaaattttg cgctccgaac gtaccggctg attatgaaac attactttat cggtaggtaa
    17641 tatttttgtt cccagttcgc tgacaatttc gccatttacc gaaatcacgc ctgcctgaat
    17701 gtattcatcc gcttcacgac gtgaacagat acctgcattt gccagaaact tgttcagacg
    17761 cacaggtttt gtcggatcta catttgaact tttatattca atctgtttct ttttgctgta
    17821 ttttgcattc ggatcatagt tggcatcctt gttgcgttta cggaaagtga taccgctgct
    17881 tctgttgtcg aaccgtgttt ctacaaattc ggaattacca gtattaccgg cttttcttaa
    17941 tctcagtttc ccttgttgcg gctgatcgtc atcataacgc ttcttgaaag caggtctgtc
    18001 accctgttct ccttttttta caaatcgcgg tctgtcatta ttgtaagggc gatcattatt
    18061 tctttcatca cggttaccaa atggtttacg gtctgagttg tatggtcttc tgtcgtcacc
    18121 accgcggttt tctctgttgg caaatggttt acggtctgag ttgaaaggtc ttctgttgtc
    18181 gtcggaattc cgaaagttgg agtttcctaa tggtttccga tcggaatttg aatggtatct
    18241 attgctatct gaactttttc tttctccaaa tctggtgtta tcatcttgat cgccagattt
    18301 ttttacagta aagcggggtc ttccgctgtt ttcacttctt aattcattgg ggttatggcg
    18361 tttgtcgcct ctttcatcgt tgtcgtggaa attgtttcct ctacttttaa gcactgattt
    18421 aacatcacgt ttttcgtacg aattaccgtc acggctggct ccggaagcac cgcttcctcg
    18481 ccgattttca aagtttgctt tcattagttt ctttagaaaa aatatttttg caaaggtcgg
    18541 taaaaaatct ggattttcag caaaaaaatc ataatcagac aataatattt atagtatttt
    18601 gtacatattg atttaataca caaaatgaaa gtcaaataaa ttcagattaa aaatttaaaa
    18661 aatagcaatc tgtttacgta agaaataatt atagtaaggt taataatgtc tgcaggcgaa
    18721 aatgaaatat gacagtaagg ttatctctga cgaatatttt taaagtaggt tatggataga
    18781 aaataaaata tatttagtaa ttttgttctc ttgcaattta tttatagcag gtatttaaag
    18841 aattaaaacc aatttaaaat atagaaaatg aaaaaattga tttttatttt tatgttattt
    18901 atagccactc taagtggata tgcacaaatg cttatgtctc ccgacggaaa tttaacgatg
    18961 aatttctcgt tgtcgtcagc cggagaacca ctttaccagc tatcttacaa aggtaaagaa
    19021 gtgattaaac cttccaaact tggtctgaaa ttgaaagata caacctggtt gacttccgga
    19081 tttactttaa aggataataa actaagttcg ttcaacgaaa cgtggaaacc ggtatgggga
    19141 gaagtcagtc agatcagcaa tcattataat gaaatgcttg taacgctgga acagaccaca
    19201 caaaaaagat ttatgaaaat ccgttttcgt ctgtttaatg atggactggg atttcgttat
    19261 gagtttgaca ggcagcccga gctgagttat tttcaggttc tggatgagtg taccgaattt
    19321 gcactgacgg gtgatcataa agcctactgg ataccaggag attacgatac aaatgaatat
    19381 ttttatacaa ccagtaaact ttcagaaatc aatgccgatg ctgtgaatgg atcaggcatc
    19441 ggtacacacg gatattttgc aaagaatgct gttcagtcgc cgctaatgat gaagacagcc
    19501 gatggccttt atataaatat ctttgaagcg gcactgctcg attattctgc aatgcatcta
    19561 ctggttgatc aggaaaactt tgtattgaca gcatccctgg ttcctgatgc ctacggtaca
    19621 aaagtatata tgcagacacc tcacaactcc ccctggagaa cggtaattgt aagcgataaa
    19681 gctgctgata ttctggcttc aaaaacaata ctcaacctga atgaaccctg caaaattgaa
    19741 aatacggatt tcataaaacc acagaaattc gttggtatct ggtggggaat gcatgtgccc
    19801 gactactgga cctggaatta ttccgacact ccaaacctga agttgaaaaa taccgactgg
    19861 aatgcactga aaccgaatgg tcgtcatgga gcaactaccg aaaatgtgaa acgatacatt
    19921 gatttcgccg cggcaaacgg aattagcgga gtacttgttg aaggctggaa tataggatgg
    19981 gaagactggt cgggaaactg gaaggaagaa gtttttgatt ttattactcc atatcccgac
    20041 tttgatgtaa aaggtttgca gaactatgca gcatctaaag gtgtgaaact gattatgcac
    20101 cacgaaacat ccgcttctgc ctcgaattac gaacgacgaa ttgataaagc aatcgaattt
    20161 atgaaacaaa acggctacga tgccgtaaag accggttatg tgggctggat tattccgcgc
    20221 ggagaaaaac atgacggtca atggatggta aatcattatc agcgtgtaat agaaaaaatg
    20281 gctgctaacc agataatgat tgatcagcat gagcctgttc gcccaactgg tcagtgccgc
    20341 acttatccca atctgatggc ttgtgaagcc gcacgaggaa atgaattcaa cgcctggagc
    20401 gtgggcaatc cgccagaaca tgaaaccatt cttcccttta cacgtctgat gggaggccca
    20461 atggattaca cgcccggagt tttcgaaata aaaatggatc attacaaaac cggtagtaca
    20521 aatcaggtac ataccacact tgccaaacaa ttggcgcttt acgtcacgat gtacagtccg
    20581 ttgcagatgg ctgccgatct ggtggaaaac tacgagaaaa aaatggatgc ttttcagttt
    20641 atcaaagatg tagccgtaga ctgggatgat accaggtatc tggaagctga acccggcgat
    20701 tatattacca ttgcccgcaa agcaaaaggt acaggagact ggtttattgg cgcaattacc
    20761 gacgaaaatg cccgcgtcgc gaatatagat ttcagttatc tggaaccaaa aacatggtac
    20821 ttggcaacca tttatggcga tgcacccaca gctcattgga aagataatcc gatggcttac
    20881 gaaatccgga aagtattggt tcagaacact tctgtgttga aacagcaact tgcagccgga
    20941 gggggagtgg ctatcagcgt gaagaaagca gcaaaaactg atttaaaagg actgaaaaaa
    21001 ttaaaataat atatcagagc gaattcataa atttctcaat ccgaaccgct acccgcttat
    21061 gggtggcggt tcctgtttta tcacccttgg cattttcaac atcaatctga aattcgtata
    21121 attttccttc gtgctttacc agtttagcgg tgcatgtaac cgtttcgcca ggttttgatg
    21181 ccctcagatg ctggacattt atttctattc ctacagttgt aaatcctgtt tcaatattct
    21241 caatcgcctg aactgctgtg ttttccataa gtgcaatcat tgctggagtt gcataaactt
    21301 cgaggcttcc ggaacccatt gctgtagcgg attcatattc ggtaatcaga tgttgttgtg
    21361 tgtatgtttt cattttgtgt ttttttcagt ttataatttg aaatcagtat gatttctgcc
    21421 ggaaaataat ggatacagat ataagctcag tccgaaaaat gtaaatgctg aaataaatcc
    21481 tgcaatcaca tcaatgacat aatgagctcc gagatataca gtggaaaaaa ccagcaggac
    21541 atatacaggc gacaatataa gggctaattt atattctcgt tttgcaagta acatgattat
    21601 tacaagcgtt gaatttccga tatgagaact tggaaatgca ccggttggtc gttcagcatg
    21661 acctctaatg aaatcaagac aagattgaaa tattccgctg ggagatgtat gtcgaagaca
    21721 gatatagttg ttgctgaaat aatctactat tgaaggataa attccggctg aaatctgttt
    21781 attgtcaatt aattgaaaat aatactgcgg accggatgca gggataagta aaaacagcag
    21841 gtaaaataaa aagaaagtta gaacagtggt aaaaaagaaa aactcagcat actttctgtc
    21901 ggtatgatag aaataaaggc aagtgaaaat cagcaacgga aaaaaagaaa tatatccaaa
    21961 attcataagt tcggagaacc aatgctgcgg atatacctgt gcaaacatca gagagggttg
    22021 aaaaccaaat attaggaagt ctgtacgtgc cagcaggtga tcaaaatttt caaaataccg
    22081 attgaggtaa aatgtttcgg tataccagta aataaacaat gaagcaataa aaaggtaccg
    22141 gataacattt attgatgatt ttttgcggga gttgcccaaa tatgccagaa agaatatgac
    22201 ggcactaatc agcagcctga taccgataaa agagacaccc gttccaaatt tcatgcagat
    22261 ataaaaagct acaaaagacg aaataatgat ataatacaga gttaattttt cagcaggatt
    22321 aaattttatt gagcgtaaat acattgaatg gaatagatat atttgaaaaa tatgattgtt
    22381 tattttatta gtcaaaagta tgactttttt gttaaattca aatttttata aacacttttt
    22441 ttgaataaat taaaaaagaa aaacgcaggg tggtaaaccc tgcgcctatc agaaagtgtt
    22501 ttttacataa actatttttt cagaggaatt ctcatcttat agaatgatct ccatacaaaa
    22561 atcaatgcaa taaccaggaa tattactccg atatatccac tgtaatcagt accaatgcct
    22621 ttcagggcgt caagtttcat tttcagcgag atttcagttg ccaataatcc gaacagagtt
    22681 gaaaatttga tgattgggtt cagggatact gatgaagtgt ctttgaatgg gtcgccaact
    22741 gtgtcgccaa taactgcagc ttcgtgcaac ggagtgtttt tttctttcag atccacttca
    22801 ataacttttt ttgcattatc ccatgcacca cccgcatttg ccatataaat agcctgaaac
    22861 aacccgaagc aggcaatgga aatcagataa gctacaaaga agttactgtc aaagaatgcg
    22921 aaaccaagtg taaagcaaat aagagcaata aaaatgttcc acatgccgtt ttgtgcatac
    22981 tgagtacaga tttttacaac ttcttttgag tcttcggtgt ttgcctccgt cttgtctagt
    23041 ttgaatgtac gtttgatgta ttccacagcg cgatatgctc ctgttgttac agcctgcatg
    23101 gaagctccgc tgaaccagaa aattactgcg ccaccacaaa tcaaaccaag cagaatagga
    23161 gcggaagtga gcgaaacgct caacatgccc acgctttcta gcagaaggat gatggagaaa
    23221 atcattgtgg ttgcaccaac tactgcagtt ccgattaata ccggtttggc agtggctttg
    23281 aatgtgttac cggctccgtc gtttgcttcc agataatgtt tacccatttc aaagtcgggc
    23341 ttgaagccgt agtctttttc aatttcttcc gaaatgccat caatttgttc aatctgcgaa
    23401 agctcaaaaa tggattgagc attgtcggtt acaggaccat aactgtcaac cgcgatggta
    23461 accgggccca tacataggaa accaaaggca accaagccaa aagcgaaaat agtggcatga
    23521 ggtccgagta cagcttccag attgccggta atgctgataa agtaagcacc tgtcattaat
    23581 ccaacaatca ataatcctgt ccagaaagca ccaaaattac ccgatacaat acctgataat
    23641 atattaagcg atggtccgcc ttcgcgcgat gctgttacaa tttcattcac atgagctgat
    23701 ttcgagctgg tgaaaaactt agtaaattcg ggaattaata cagcagccag tgtaccacaa
    23761 ctgataatac ctgccagttt ccaccacaat gttgagtcgg gtaaatctcc aaccagtaag
    23821 tgactcatga agaaagaggt agaaatacta aggatagcag caatccagat tagtcgcatc
    23881 aaaggttctt cgaaatcaaa tattttcatt cctttatatt tgcgttttga aatagcctgg
    23941 ttgatgaaat acgaaatacc ggaacataag tccatgagga aacgcatacc gaaaatccat
    24001 acaataagtt ttgcctgaat atcaggattg tgtactgcga gtgtgataaa ggtaatgaga
    24061 gcaacaccgg ttacgccgta ggtttcaaaa ccatcggcag taggacctac gctgtcgccg
    24121 gcattatcac cggtacagtc ggcaataact cccgggttac gtgggtcatc ttctttcacc
    24181 ttgaaaacaa ccttcatcag gtcagagcca atatcagcta ttttggtaaa aatacccccg
    24241 gctatacgca atacgctggc accgagcgac tcaccaatcg caaatccaag gaagcaaata
    24301 ccaacaatct cgcggggtac aaatagcaga ataaccacca tcataaccaa ttccaacgag
    24361 ataaggaaaa gtccgacact cataccggag cgtaaaggaa tgttcacaac atcccacgga
    24421 ttagcgcgca gcgaagcaaa tgctgtacgt gaatttgcat aattgttgat tcttatgcca
    24481 taccaggcaa ctgcataaga gcctcccata ccaagaatcg agaaacaaag aacaaacaac
    24541 agtgtgccta ccgatttacc ctgaagtgca aggaaatagt aagccatgat gacagcaaca
    24601 aatgcaaaaa gcattaaaag aaatttgccc tgttgcagca aatatgtttt acaggtctga
    24661 taaattgttt cggaaacttt cagcatcgat atatgtgctt tctgtttctt tacctcatgg
    24721 tgtaaccaga ggcttatacc caatgtaaag cagattacca acgcgccata gaacaaaaag
    24781 ttccatgcag taatttgatt tccgaaaatg tgaaatgttc cttcatgcaa atcagggatg
    24841 gcaatgtcag cttcgctggc aaaggtactg accgacacaa aaagactcga aaggatgagc
    24901 attactgctc ttttcatgtt tttcatacaa aatgtgtagt ttgatattta taggtttaaa
    24961 aaattaaaaa aaaacgccca aagttagtaa taagaaattg aattagtttt acgataatta
    25021 ttaaaaatac gttaaataaa tacttttttt tcgcaaaatt tggtggtttt tgaacaaaat
    25081 gaaatttcat gctaaacaat aattcaaata aacaccgaag ctaactgatt ggtatcgggg
    25141 tttatttgat aaatctcatt ttacaaaaaa cgaaaaaata aaattttgag cattctgtat
    25201 attttctttt cgttttgaca tttgcacaca gatgaatatt cttgtaacag gttgtgaaaa
    25261 tgctatcctc tatcaaattt acaaaatact ttattttatt aaactttata cattaattat
    25321 agataacttt ctattttgat tatcagtgtg ttgagaaagc ccccccctaa gtgttgataa
    25381 cttgtcataa atatttaatc taataattag gtttttacgt attttttgat ttatttttgc
    25441 aacgatttaa taaaaaaaat ataaacgtta aaaaaatttt ttactatgtt tatgaaaaca
    25501 aaactatttg tcttatcaat tttattgatg agttttactg ctttgaacgc ccaggtaacc
    25561 acgtcaagta tgagtggatt ggtgaccgat gctgaaggag ctgttgttgg agcggctgtg
    25621 gttgctacac acaccccttc gggaaccact tacagaacca taaccaatgt agagggtcgc
    25681 tataatctaa acggtatgcg tgtaggtgga ccttattccg tagaaattac ttacgttggt
    25741 tacggtaaaa gtacaaccaa caacattacg ttgagcctgg gggaaaacta tgccttgaat
    25801 gtggtgctct cagaagaaac atcaacattg gatgaagttg tagttactgc aattcgcaca
    25861 aaattcacta ctgagaaaac cggtgcggtt acaaatatca caaacaccca aatagctaat
    25921 ttacccacag ttagccgcag cattattgac gttactcgcc tttctccata tggaggtaat
    25981 ggtatgagtt tcgcaggtac tgatggtcgt acggcaaact ttacagtgga tggagctaat
    26041 tttaataata actttggtct gagtgacaaa cttcccggtg gaggtaaccc aatctctatt
    26101 gaagctattg aagaattgca ggttgtaatc gctccatacg atgttcgtca aactaacttt
    26161 ataggtggtg gtgtaaatgc tatcaccaga tctggtacca ataccttcaa aggtagcgct
    26221 tacgcttttc atagaaatga aaacttacgc ggtgatgcta taagaggcaa acaaattaca
    26281 ggagcacgtg ataaagaccg caatacaaca tacggtttta ctttgggtgg cccaattatc
    26341 aaaaacaaac tgttcttctt cgtaaacggt gaaatggcaa aaacaccaac aattgctaat
    26401 cgttggagac cttcaactaa tggtgttgct gatgcaaaca attatatttc acgcacaaca
    26461 gtaagcgact tgcagacggt ttctgattat gtaaaaagta aatatggata tgaaacagga
    26521 tcttatacag attttccggc tgatgaaagc aattacaaac ttcttgcacg tttggactgg
    26581 aacattacaa accaacatcg tttagcttta cgctataact acacaaaaaa cctctcatgg
    26641 gtagctccta atgctacttc aatggatggg ggtgatcgta tgacggaagc ccgtttgtcg
    26701 caggcttcaa tgtcatttgc caattcaatg tattcaatga gtaataaggt tcattcggtt
    26761 tctttggact tgaacagccg tttgtccgac aacttatcaa atcaattttt agcaacattc
    26821 tcaaagctcg acgatgtgcg cggctctaat tcagacgaat ttccttttat tgatattttg
    26881 aaattaaaag aggacccaac cggttgggat aaacctgtac aacagtccta tatgtcattg
    26941 ggctatgaac ttttcacctg gaataatgct gttcacaaca atgtatggaa cattaaagat
    27001 gaattgactt attatatagg taatcataaa attgtaggag gaatcgccta tgaatataaa
    27061 atggcagata atgcttatat gcgtaacggt acaggatact accgctactc aagtcttgac
    27121 gacttcctaa acgaagcagc tcctgaaatt gtagacttaa cctatggtta cgatggagag
    27181 agcaatcctg ctgcacgcgt aagaacaagc aaacttggaa tttacggtca agatgactgg
    27241 agtgtgaacg acaaattcaa actttcgtac ggacttcgta tcgatggact tttctttaat
    27301 aataatgact tgataacaaa taatgctatt ctggctcttg attattccgg ccgtaatatt
    27361 gatacaggta aatggcctgc aaccaatatc atattttctc cccgtattgg ttttgtgtgg
    27421 gatgcatttg gtgataagag cctgaaagtt cgtggaggca ccggtctttt ctccggcaat
    27481 ttaccacttg tattcttcac caatatgcct accaacggtg gaatggtaca atatcaggca
    27541 caaattagtg gtaaaactaa ggtttttgat gcaaagaaag gcgaaattgt taaaggttac
    27601 acaggtacat atactactga caaggatgga aataggtatg ttgatatgtc gcagtttgcc
    27661 ggcggtcttg taaaagatgc aaacgggaaa gccactactg ctgcattgta cgacaaatta
    27721 atatcattgg ggtatcctgc cacaatttca cccgaagatg gaactgttcc ttctgctatt
    27781 gcggctgttg atccaaattt caaaatgccg gaagtgtgga aaacatcact tgctgttgac
    27841 tacgcgttta aaacatcttt tccattctct gtatcagtag aaggaatctt caacaaaact
    27901 atcaacggtg tttctatttc agactggagt atcccgtcgg ttggtggttt cgcccgtttc
    27961 aatggtgttg acaatcgtcc ggtatattcc gcgggttatc gtgtcggtac aaaggcattt
    28021 gtactggaaa acacaagccg cggttatggc tggtcaggca acatcacttt caatgctcaa
    28081 cctacaaaat ggcttagcat gatggctgcc tatacacaca ctgtagctaa agatgtaacc
    28141 ggaatgcccg gatcaaatgc ggaatctgca tttacatatg ttcctacagt agaaggacct
    28201 aaccacatta aattgcacaa ttctcagtat aacacgcccg atcgtttggt ggcttcactt
    28261 acagccaacg acaagagtgg caatcattat agccttatct atgaagggtg gctcggtggg
    28321 gctaactact catacatgac cgcaaatgat atcaatggtg acggttacaa ctacgatgca
    28381 atatatgttc caacggatga cgaagttgca aataatcaat tccgttttgt ttctgaaagc
    28441 gacaaaacgc gctttatgga ctatgttcat gccaatgatt atttgaaaaa ccaacaaggc
    28501 aagtatgcgg aagcctatag cgtttactct ccctgggttc atcgtatcga ctttggctac
    28561 aaacacgatt ttgctcttaa ttcaggcaaa aacaaacaaa tgctgcaact tagcttagat
    28621 gttaaaaatg taatgaacct gttcaactca agctggggtg tagctaaata tctgaatccg
    28681 gaaattggtt ccgaagctcg tgttctcaaa tatgaaggtg ttgatgctga tggatttgca
    28741 acattctcta ctccatcttc aataaaaggt aacacacaaa cattcacacc aagctactct
    28801 ttgggtcaat gctggtatgc ttcaattggt atcaagtata tgttcaacta attaaaaata
    28861 agaaaat
//
LOCUS       DAOPYB010000020        28763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1626_length_28763_cov_8.488575, whole genome shotgun sequence.
ACCESSION   DAOPYB010000020 DAOPYB010000000
VERSION     DAOPYB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 28763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28763
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1626_length_28763_cov_8.488575"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PKH58_02970"
     CDS             complement(<1..308)
                     /locus_tag="PKH58_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX88020.1"
                     /translation="MHQNVNKFLRFVFSGLLFLAIIPAVRAQTVLLDPPEVYLGATGG
                     MTGSMVMFNPKVNQTMPLLGYNGGLSFRFVTESHCGLQIEANYSQRGWDEASGNYSRR
                     A"
     gene            complement(289..>1311)
                     /locus_tag="PKH58_02975"
     CDS             complement(289..>1311)
                     /locus_tag="PKH58_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88021.1"
                     /translation="AYLLPFMLDSEKKDPTVDKFLEFYMGSLLAINNVKNGDFKIDIY
                     TYDTEKTEVRINEILNKPEMQKMDLIIGPAYTAQIPVLTDFSNRRKINTIIPFSSNIN
                     NIETNPYVFQFNPDNDIQKVFAADQFRYKFNDANIIFAETNNGKWSEDGTDFFKYLLQ
                     KLDKSNTPYTRIGSDILSLKTIESNLRTDKRNIIIFNSEEYSSIQIYLSKLFDLSSLY
                     DVSVLGQYSWRNKSGKKPKMYYIAPFNPDINSVEKNLYESNYKLYYKQFHSDKNPRFD
                     MLGYDLTNGFLKQMDNTGFKFNEKSKTLKFDGGIQSGYNFKRTSDGGGFINQQLYLIE
                     DAPKRK"
     assembly_gap    1312..1411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1412..1842)
                     /locus_tag="PKH58_02980"
     CDS             complement(<1412..1842)
                     /locus_tag="PKH58_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNX88022.1"
                     /translation="MRIKISTLLLVLLIICSAIIYAQKSEIPTKIIDGKEYYIYQVEA
                     GEGLYSISKKFGVSQADINNANPQIYDGIKAGQEILIPKTGKVKLSTTRIKDEDEGFV
                     LHTVEKKQTLFAISRKYNVTQEDIREANPQLTEGLKTGDVLR"
     gene            2083..>2606
                     /locus_tag="PKH58_02985"
     CDS             2083..>2606
                     /locus_tag="PKH58_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88023.1"
                     /translation="MKHFFLLLMMSVLAVNTYSQIQVKGNFTVYNEATGLDYIYIFST
                     IDNAAEIHYTTTENTNQVKWYTFLNGVKTEMTSVSIVSPTETYVDPKNNTGYIINVNG
                     KDVVRFWVFDYSQYVPAFTSISATDGETPCENIQLNLTGNLPEFKYQNTTGGIFSIDR
                     YFDVEYNTLEWDKD"
     assembly_gap    2607..2706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2707..3153
                     /locus_tag="PKH58_02990"
     CDS             <2707..3153
                     /locus_tag="PKH58_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX88024.1"
                     /translation="PNVRSYFWTIYRNGEKLMARSEKDHLYTFSKAGNYKIELNVTNG
                     ICSDSSSVNVVVMESSIIAPAVFTPNNDGYNEEFRVAYRSIIEFHCTIYNRWGRVVYT
                     SSDPAKGWNGTIGGKQAAEGTYFYIVNARGSDDKVYKLKGHVNLLR"
     gene            complement(3208..4278)
                     /gene="gcvT"
                     /locus_tag="PKH58_02995"
     CDS             complement(3208..4278)
                     /gene="gcvT"
                     /locus_tag="PKH58_02995"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNX88025.1"
                     /translation="MKTTPFTDIHIALGAKMHEFAGYNMPIEYSGINDEHITVRTGVG
                     VFDVSHMGEIWVKGPKALDLIQKLCSNDASKLTLGQAQYSCFPNGKGGIVDDIIIYYF
                     EPEKYMLVVNASNVEKDWNWLNDNNTEGAILENASEHTAQLAVQGPKATELLQRLTSV
                     NLSEVPYYHFTVGDFAGVPHVIISNTGYTGAGGFELYFYPEHGVNIWNALFETGKDLG
                     IKPIGLGARDTLRLEKGFCLYGNDIDDTTSPIEAGLGWITKFAEGKNFTDRALLEKQK
                     AEGTTRKLVRFELIERGIPRHGYEIVNTDGVKIGHVTSGTMLPDSKKGIGMGYVNAPY
                     FKNGEPVYVQIRNKNLEAVIVK"
     gene            complement(4386..5612)
                     /gene="pepT"
                     /locus_tag="PKH58_03000"
     CDS             complement(4386..5612)
                     /gene="pepT"
                     /locus_tag="PKH58_03000"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNX88026.1"
                     /translation="MNITDRFLKYVSFTTTSDEKTNLTPSTPGQMIFAKYLVDELKSI
                     GMKEITLDKNGYVMATLPANTDKDVPVIGFISHMDTSPDASGKNVKPRVIENYDGNDI
                     LLNEEKLILFETDKYPEILQYKGQDIIVTDGTTLLGADDKAGLAEIVTAMEYLTEHPE
                     IKHGKIRIGFTPDEEIGQGADHFDVKKFDAEWAYTMDGGEIGELEFENFNAASAKVTI
                     TGLNVHPGYAKHKMKNSMRIATQFVSMLPRHETPEHTDGYEGFYHLTNIEGTVEKTLL
                     SYIIRDHDRDRFERRKKEIQHLANKINAEYGENTAVVEMRDQYYNMREKVEPVMHVVD
                     LAFEAMKAVDVKPNVKPIRGGTDGARLSFEGLPCPNIFAGGHNFHGRFEYIPIPSMEK
                     AMMVIVKIVELLEKKS"
     gene            complement(5723..6643)
                     /locus_tag="PKH58_03005"
     CDS             complement(5723..6643)
                     /locus_tag="PKH58_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX88027.1"
                     /translation="MAIPTTRTKEEGIISIDNELCNGCGLCVSVCKDFSLIIENGKAV
                     ISGTPVFGCISCGHCMAICPKGAIKITGRTLSPDDLFELPPANSVAAYDHLLSLLQRR
                     RSIREFRDQPVEDEIIEKILAAARTAPMGLPPTDVNVLILNGKEINTEFVKDFSEMLK
                     SMKWFASKPVQALMRPFISRESAELYKSFISPLFDFFINNNEEGKNWITYGAPLVMYF
                     YGSPYCDPADPIIAATYAMVTAESLGLGTCMIGSVHPFIQYGKKAEKLREKYLIRYKS
                     REGLFVIFGYPSVHYSNGITRTFASETILK"
     gene            complement(6693..7820)
                     /locus_tag="PKH58_03010"
     CDS             complement(6693..7820)
                     /locus_tag="PKH58_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNX88028.1"
                     /translation="MLTYIQNKEIEQLYTTPIVQQTAFWSVVKKKLGATTLAINFNSR
                     KSDLYNIPQSEDDVIVSDVLVIVRYIDQNHSIAYVPYGPELQPDDEFQGLFLEELSEC
                     IRSFLPKNCIMIRYDLCWESYWAKEQNYFDPDGNWIGEPQISTQEMRFNFNTVNWNFK
                     KTSSNILPSNTIYVDLNKDIDEILATMKPKTRYNIGLAERRGIVVKSSGIENIDIWYK
                     LYQETAQRNHILTNDIKYFRAVLSAKTNDMRSPAEVKLLIAEYNNQPLAAMFLIITGN
                     RGTYLYGASSSENRNLMATYALQWEAIKISKASGCTEYDMFGVSPNPDPNHPLYGLYR
                     FKTGFGGEIYHSLGCWDYPLDVEKHRHLIALELLEQGYHLN"
     gene            8055..10199
                     /locus_tag="PKH58_03015"
     CDS             8055..10199
                     /locus_tag="PKH58_03015"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNX88029.1"
                     /translation="MRHSIITILILFCFIPFISGNATLPLDSVLYRTMNAAETYSRLI
                     SDYEAEVYMLAYVKTHKKNFLYKYTYLVPRFVLHDPKNDEGLIESVSKLKMSSSNIYY
                     QDINLINGTMTRKSEIGMLPFKFMSVNVYKEAAPDESYLLPLRKSSAKYYRYKLLSCD
                     SLKHKPEYTIGFTPRYKNSKLISGSFVIEENTWRVKQFIGKGSDLFLRFTFNITMGDD
                     ANNQYLPVNFTINQSYVYFGNKVTNRYNALLTYKNVNFNGAKDHLNNLNLGNRYKIRL
                     DSVPVNIDSAYWNKIRPVPLSDNEKNLMAKYLEKRRIIQSQKKEVDTVALKTRNALTF
                     AKNMVMNTKYSYKSTNIRYSGILNPSMISYSTEDGLTYRQKLYLGIELERHQRININA
                     FAGYVFKKKEFYGDLSTVWNYDPAKYGNLSFSIGKGNQSYSSLFLKMVQDSLTSQGLK
                     FTDLGVNYFKDYYMRIFNSVEISNGLQLGAGFDYHIREPNKKQMNLTAPNHDQMDNMF
                     DTRYYFVPIISMSWTPEQYYTMDKNQKVYVRSDYPTFKIQYAHSLKNIFGSTSQYSRV
                     EFDINQNIRLDLMKSIQYHLGTGFFSRQKTEYFTDFVYFRRSYFPETWGEGIGGVFNV
                     LPRSLYNASSSYVQAHLMYETPRFLFTRIPGLSGGVVKERLYFSQLYTPTIPSYSELG
                     YGVGNRFLNAAIFVSFFKLKYQDMGFKTVFLL"
     gene            10330..12150
                     /locus_tag="PKH58_03020"
     CDS             10330..12150
                     /locus_tag="PKH58_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNX88030.1"
                     /translation="MQKTYLLTDFLPTTKKELELRGWDEVDVILFSGDAYVDHPSFGA
                     AVIGRMLEFEGLRVALVPQPNWQDDLRDFRKLGRPRMFFAVSAGNMDSMVNHYTANKR
                     MRSDDAYSPDGRAHMRPDYATITYCNILKKLYPDVPVVIGGIEASLRRVTHYDYWSDK
                     LMPSILIDSKADFLIYGMGEKPLKALLEELKNGKKFGEITDIPQTSFLSDKIPGDDNL
                     QTIELVSHEECLKDKKKYASNFRYVEEESNRMNGARLVQKTRGKYLIINPMYPPWKPE
                     ELDATYRLTFTRLPHPKYKGKQIPAYEMIKFSVNLHRGCFGGCAFCTISAHQGKFIVS
                     RSKQSILEEVRQIAQQPDFKGNLSDLGGPSANMYNLHGIDMKICEKCKRPSCIHPKVC
                     PNLNINHSALTDIYRSVDKIPGIRHSYIGSGIRYDMLTAETKNAETTKSQLDYMRELV
                     SKHVSGRLKVAPEHTSERVLKLMRKQSFQDFKKFKVLFDRINREEGLNQQLIPYFISS
                     HPACMNEDMAELAAITKDLNFKLEQIQDFTPTPMTLATEIYYSGFNPYTLEPVFTAKS
                     KDEKLEQRKFFFWYKKEYHQQIMRDLQKMNRQDLIKKLFQ"
     gene            complement(12201..>12335)
                     /locus_tag="PKH58_03025"
     CDS             complement(12201..>12335)
                     /locus_tag="PKH58_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNX88031.1"
                     /translation="ELVAGDNFGQIFITDTNRKHLDEILAQIGQSSQIFEIVNGEIIR
                     "
     assembly_gap    12336..12435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12436..13166)
                     /gene="recF"
                     /locus_tag="PKH58_03030"
     CDS             complement(<12436..13166)
                     /gene="recF"
                     /locus_tag="PKH58_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298314.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNX88032.1"
                     /translation="MRLNSISILNYKNICQADLDFPAKINCFVGNNGMGKTNLLDAIY
                     YLSFCKSYSNSIDSQNIEHNADFFMLQGKYSLRDTEEEIYCGMKRKQKKHFKRNKKEY
                     ERLADHIGLLPLVLVSPGDDELISEGSDERRKFMDSVISQYNKTYLNALLNYNNALKQ
                     RNALLKEETLFDETLFEIWEELMVKHGKYIYECRKSFIDEFIPIFQEIYRFISCGNEQ
                     VSLNYKSQLAEADLSLKLKQNRERDR"
     gene            13368..15491
                     /locus_tag="PKH58_03035"
     CDS             13368..15491
                     /locus_tag="PKH58_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNX88033.1"
                     /translation="MISDQLIKLLSASCQLSTRQTLNALKLLEEGSTIPFISRYRKEA
                     TGGMDEVQLADLKEQYDKLCELAKRKATVLKSIEEQDKLTPELQKRIEECWNSSELED
                     IYLPFKPKRRTRAEIAREKGLEPLAKIIMKQQSDDIESVAGRFISKDVADTEVALKGA
                     RDIIAEWVSESEIARNLIRKQFAYEANISSRLIKGKEEEAQKFRDYFECSEKLARCSS
                     HRLMAMRRGEAEGFLRVNISADDDKCITKLLRIFQKSDNECGLQVAEAVEDGYKRLLK
                     PAIETEFAAESKEKADQEAIRVFTENLRQLLLAPPLGQKRVMGVDPGFRTGCKIVCLD
                     EQGNLKHNENIYPHPPVNEHSQAMRKLQKMVEAYDIQAIAIGNGTASRETEQFIQNIR
                     FDRKVQVFVVSENGASIYSASKVAREEFPEYDVTVRGAVSIGRRLMDPLAELVKIEPK
                     SIGVGQYQHDVDQVKLKKSLDQTVENAVNSVGVNLNTASKHLLTYVSGLGPQLAQNIV
                     NYRAENGSFTNRQELLKVTKMGAKTFEQCAGFLRIANGSNPLDNSAVHPESYSIVNQF
                     ATDLKMDINQLIGNNSLKSTLDLTKYVTDTVGLPTLNDILEELEKPGRDPRSAIKVFE
                     FDPDLKDIKDLRTGMELPGIITNVTNFGAFCDIGIKENGLIHISNLSDRFVSNPADIV
                     SVHQHVRVSVIDVDMTRKRIQLKLL"
     gene            15512..16228
                     /locus_tag="PKH58_03040"
     CDS             15512..16228
                     /locus_tag="PKH58_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX88034.1"
                     /translation="MKKTISCLILLLIGFSGFTLKAEENAVINAEQTMINESLQLNKN
                     SILFSSLVWDKNKITLKKNKSDFGKQLWKNANFGWNTYKTDDYKWRNILIGTGYGRNF
                     EHVKKAYWMVGLNLNWSKYTLYPDGAFNCTANKDYLKTTSLSVPVTAGYTIYTSKLRS
                     MNLKAFTGPVFEYILTSKLNGYTDNLHPINNFQTGWSAGANFRFLYLLSARVTYTYYP
                     NSLFENHDLYRRSLSFSLGF"
     gene            16352..17227
                     /locus_tag="PKH58_03045"
     CDS             16352..17227
                     /locus_tag="PKH58_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNX88035.1"
                     /translation="MLQSMTGYGKASCEINNKKIVVEIKSLNSKQLDLSTRIAGIYRE
                     KDIEIRNLISQKLERGKVDFSLYIDNSGKDSGSKINQAVIKSYYEQLKALSGDLEIPE
                     STDWMSILMKMPDALKTETEELDENEWIEILKSIETAINQLIEYRRQEGKTLENVFTL
                     KIGRIGEFLVEIEPFESERVDKIKQRLDENLAALSDKIDYDKNRLEQELIFYIEKLDV
                     NEEKVRLKNHLEYFIKTMKNEPSPGKKLGFIAQEIGREVNTLGSKSNHSEMQKIVVKM
                     KDELEQIKEQVLNTL"
     gene            17276..17842
                     /gene="gmk"
                     /locus_tag="PKH58_03050"
     CDS             17276..17842
                     /gene="gmk"
                     /locus_tag="PKH58_03050"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNX88036.1"
                     /translation="MQGKLIIFSAPSGAGKSTIVNYLLSRGLNMEFSISATSRAPRGN
                     EQYGHEYYFISTEEFKERISKGEFVEYEEVYSGCFYGTLHNEVERITSSGRNVIFDVD
                     VKGGLNIKKQFGDRALAVFIAPPSIETLRQRLIKRSTDSREMIEKRVGKAAYEMTFAP
                     QFDVVILNDNLETAKMEAEKVIKEFFER"
     gene            17874..18458
                     /gene="nadD"
                     /locus_tag="PKH58_03055"
     CDS             17874..18458
                     /gene="nadD"
                     /locus_tag="PKH58_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNX88037.1"
                     /translation="MKSIGLFFGSFNPVHLGHTNLAEYIFRFSGVDEIWFIVSPRNPL
                     KEQSELIDEHLRLKMLQLATAETEYLIASDIEFYLTKPSYTINTLKTLSEKYPEDNFI
                     LLIGSDNMQIFDQWKDYQTILDEYSVVVYPREGYPYEDFEEIYPDMQILEEAPLFEIS
                     STELRQLIAEKKDASQWLHPDVYQFILENGLYLS"
     gene            complement(18478..19113)
                     /locus_tag="PKH58_03060"
     CDS             complement(18478..19113)
                     /locus_tag="PKH58_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX88038.1"
                     /translation="MTVKTFTFSPFQENTYLAYDETGESIIIDAGMFYEREKAEIVKY
                     METHKLSLIKVINTHLHLDHQFGNKFLFDTFAIAPEASEEDEFLLATAHQSAQMFGLP
                     YKEAVQPLGKYLTDGDEIKFGNSSMICLSVPGHSPGSLAFYNQKEGIVFTGDALFNGS
                     IGRTDLGRGDYSTLIRSIQEKLLTLPDNTVVYSGHGPKTTIGNEKKNNPFL"
     gene            complement(19131..19751)
                     /gene="rsmG"
                     /locus_tag="PKH58_03065"
     CDS             complement(19131..19751)
                     /gene="rsmG"
                     /locus_tag="PKH58_03065"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNX88039.1"
                     /translation="MDEILKYFTNLTETQKNQYKALFDLYLDWNSKINVISRKDIDNL
                     YERHILHSLGIAGVISFKPGSSILDVGTGGGFPGIPLAILFPQVRFTLIDSIGKKIKV
                     GSEVSKSIGLNNITLKHERVQEEKNKFDFVVSRAVMPLDDLVKLVRKNICTKQQNALP
                     NGLICLKGGELQHEILPYKNFAETYELSDQFNGEFFKTKKVVYVPL"
     gene            19951..20823
                     /gene="fabD"
                     /locus_tag="PKH58_03070"
     CDS             19951..20823
                     /gene="fabD"
                     /locus_tag="PKH58_03070"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNX88040.1"
                     /translation="MKAFVFPGQGAQFTGMGKDLYEKSPVAKEYFEKANDILGFRITD
                     LMFEGTAEDLRQTKVTQPAVFLHSVISAIALANEFNPDMVAGHSLGEFSALVAAKALT
                     FEDGLKLVYARAMAMQKACEAEPSTMAAVLGLDDETVEEVCDSIKENVVVPANYNCPG
                     QIVISGSIEGVDQACEKLKEKGAKRALKLPVGGAFHSPLMQPASEELRAAIDAAIVSE
                     PICPVYQNVNAYPQTDPVAIKQNLIAQLTAPVRWTQTVKNMITDGAVEFVELGPGDVL
                     KGLIKKVNPDVTVG"
     gene            20975..21391
                     /locus_tag="PKH58_03075"
     CDS             20975..21391
                     /locus_tag="PKH58_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HNX88041.1"
                     /translation="MDTQLLRNKELYPTEEVLQKVLMDSIQAYNQLVTTIAELQAEMI
                     WRYYNDGKSWLCKVQHKNKTIFWLSVWDGYFKTTFYFTEKNCQGIELLDIDQTIRNEF
                     RNNKPIGKFLPLTLTIQNRKQLIDLLKIAEYKRGLK"
     gene            21408..23162
                     /gene="aspS"
                     /locus_tag="PKH58_03080"
     CDS             21408..23162
                     /gene="aspS"
                     /locus_tag="PKH58_03080"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNX88042.1"
                     /translation="MFRTKTCGELRLADAGKKVTLAGWVQRSRKMGGMTFIDLRDRYG
                     ITQLVFNQDVNAELCDRANKLGREYVIQICGEVAERSNKNTHIATGEVEILVDILNVL
                     NEANTPPFTIEDNTDGGDDIRMKYRYLDLRRNSVRANLELRHKMTIETRSYLDKLNFL
                     EVETPVLIGSTPEGARDFVVPSRMNPGEFYALPQSPQLFKQLLMVSGFDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVQQEDVLNTFEGLAKHLFKTIKGIEMNEPFPRMTWH
                     DAMKYYGSDKPDLRFGMKFVELKEAVTGHDFSVFDEAEYVGGICASGCASYTRKQLDE
                     LTDFVKRPQIGAKGLIYIRMNEDGTVKSSVDKYFNEENLKNIAAKFEAKVGDLILILS
                     GPKRKTQKALCELRLEMGERLGLRDKQVFAPLWVIDFPLFEWDEEVQRFFAMHHPFTS
                     PKPEDIHLLDSDTGNVRANAYDMVINGVELGGGSIRIHDSQLQRKMFELLGFTPEKAQ
                     AQFGFLMGAFQYGAPPHGGLAFGLDRFVSLFAGLDSIRDCIAFPKNNSGRDMMIDAPS
                     KIDQSQLDELNLIVDIKE"
     assembly_gap    23481..23580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23684..24607)
                     /locus_tag="PKH58_03085"
     CDS             complement(23684..24607)
                     /locus_tag="PKH58_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNX88043.1"
                     /translation="MTLQQLEYIIALNKTRHFVQAAESAGVTQPTLSMMIQKLEDELD
                     VKIFDRSKHPIEVTPIGKKVIDLAQSTVCQIGRIKEVVSNEKGNLTGNFNIAVIPTVA
                     PYLLPQFFVHFNKKYPDLSFKVSEMHTSVLEKKLVLGEIDMAIASTPLNNPLILEIPL
                     YYEKFVAYISPDDELYRCSELNSNDLRIENLWILEEGHCFRSQVFNFCKLSPASNIYE
                     AGSIDTLIKIVDENGGYTVIPELHTNSLSENQKKNLRMLINPEPVREISLIIHQDFIR
                     EGQLNAVAETIKQIIPQHMLDQRLKKFAIKI"
     gene            24749..25618
                     /locus_tag="PKH58_03090"
     CDS             24749..25618
                     /locus_tag="PKH58_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNX88044.1"
                     /translation="MKKYNLFILILIFSLNASQAQLLWKISGHGLEKPSYLFGTHHLI
                     PIQFLDSVPGLYKAFNSTQTVVSEVVLNNLDTAPAIQRAALLPDSITIQTLLSKTDYD
                     FANEEITKTLRMSLNSLNKMHPSLIAKFYELELYKTVEHFDENTKSDSYFQLVAAQKG
                     IPVVGLETVEKQLNLLFPKNYQKEAQLLIETIRNKDKLIGEINEMNQMYRKAKIYELE
                     KLASQSNKRWNVSEEENYVLVDERNIDWIRQLPELLNKSSCFIAVGVLHLPGTNGLIN
                     LLKKEGYKVTAIN"
     gene            25736..27100
                     /locus_tag="PKH58_03095"
     CDS             25736..27100
                     /locus_tag="PKH58_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNX88045.1"
                     /translation="MSKTKKFLLPEAETPTQWYNILADMPNKPKPILNPGTKQPLKPE
                     DLYPIFAEELSRQELNMSDQWIDIPEEVRDKYMIYRPTPLVRAYNLEKALDTPAHIYF
                     KNESVSPVGSHKLNSALAQAYYCKKQGITNITTETGAGQWGTALSFAAKIFGLELAVY
                     MVKISYEQKPYRRSLMQAWGAQVIASPSMSTKAGRKIITEHPNYQGSLGTAISEAIEL
                     ALQTPNCRYTLGSVLNHVSLHQTVIGLEAEKQMEMAGEYPDIIIGCFGGGSNFSGISF
                     PFMRHTINEGRKTRYIAAEPASCPKLTRGVFQYDFGDEAGYTPLLPMFTLGHNFAPAH
                     IHAGGLRYHGAGTIVSQLMLDKYMEAVDIPQIESFEAGILFAQTEGIMPAPESAHAIA
                     SAIREAKQAKEEGVKKTILFNLSGHGFVDMAAYDQFIAGDLTNYELSQESIDKTTGLL
                     EKII"
     gene            complement(27171..28442)
                     /locus_tag="PKH58_03100"
     CDS             complement(27171..28442)
                     /locus_tag="PKH58_03100"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNX88046.1"
                     /translation="MMNFRPKDHVVIVGGGFAGMRLVKELKTTPLRITLIDKQNYHSF
                     QPLFYQVASARLEPASISFPFRKVFQNYRNFEFRMAEVKSVDTGNNMLITNNGVVHYD
                     HLIIGTGCTTNYFGNEEIKKNSLAMKTMQEAIEIRNRILLNFEAYQTAPFEAKEALMN
                     IIIVGAGATGVELAGSFAELKNDILPKDYPNVDFSKLRIILLEGSPFTLSVMSDVSKK
                     MSRKYLEDLGVEVKTGVILNSYDGNTAKLNTGETIRCSTLIWSAGVTGNVIQGVGSPD
                     NLVRNRYVVDRNNLVKDTKNVYAVGDIAYMETPDYPKGHPQVANVAITQAKNLADNIK
                     KMLKGKKTTEYEYRNKGSMATIGKNKAVVDLKFWKFQGLFAWFVWMFLHLMLILSVRN
                     KLIIFINWAWSYVTKDTSLRLIINPDEKNVH"
BASE COUNT         8700 a         5397 c         5676 g         8590 t
ORIGIN      
        1 gctcttcgcg aatagtttcc actggcttca tcccagccgc gctgcgaata gtttgcctct
       61 atctggaggc cacaatggct ttcggtaacg aaacggaagg ataaacctcc gttgtaacca
      121 agtaatggca ttgtttgatt gactttcgga ttaaacataa ccatggagcc cgtcataccg
      181 ccggtagctc ccaggtaaac ttcaggtgga tctaaaagta cggtttgagc ccttacggca
      241 ggaattatag caagaaaaag taatccggaa aaaacaaacc tgagaaactt atttacgttt
      301 tggtgcatct tcaattaaat aaagttgttg attaataaag ccaccgccat cactggtacg
      361 tttgaaatta tatcccgact gaatacctcc gtcaaatttc aatgttttcg atttttcgtt
      421 aaacttaaat ccggtattat ccatttgctt caggaagcca tttgttaaat catacccaag
      481 catatcaaaa cgcggatttt tgtcggaatg aaattgtttg taataaagtt tgtaattact
      541 ttcatacaga tttttttcga cagagttaat gtcgggatta aatggtgcga tgtagtacat
      601 ttttggtttt ttacctgatt tattgcgcca ggaatactga cccagaacac ttacatcgta
      661 taaggagctg agatcaaata atttgctgag ataaatctga atggaagaat actcctcgct
      721 gttgaatatt attatgtttc ttttatcagt acgcagatta ctttcaatgg ttttcagcga
      781 aagaatatca gaaccaattc tggtatatgg tgtgtttgat ttatcaagtt tctggagcag
      841 gtatttgaaa aaatcagttc catcttccga ccattttcca ttattggtct cagcgaagat
      901 tatgttggca tcgttgaatt tatacctgaa ctgatcggca gcaaaaactt tttgaatgtc
      961 gttatccgga ttaaactgga atacatacgg attggtttca atattattta tattggaact
     1021 gaaagggatg attgtgttta tttttctccg gttcgagaag tcagtcaaca ccggtatttg
     1081 agcggtataa gccggaccta tgatcagatc cattttctgc atttcaggct tattaagtat
     1141 ttcattaatc cgtacttcag ttttttcagt gtcgtaggtg taaatatcga ttttgaaatc
     1201 gccatttttc acgttattaa tggcaagcag tgaacccatg tagaattcaa gaaatttatc
     1261 aaccgtagga tcctttttct cgctatcgag catgaaaggg agtaaatatg cnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcagcaca tcgcctgttt tcagaccttc
     1441 ggtaagctgt gggttagctt cacgtatatc ctcctgtgtg acattatact tgcggcttat
     1501 tgcaaatagt gtttgttttt tttctaccgt atgaagtaca aatccttcat cttcatcttt
     1561 aatccgggtt gttgatagtt taacttttcc ggttttggga attaatattt cctgtccggc
     1621 ttttatgcca tcgtaaattt gtggatttgc attattaata tcagcttgtg aaactccgaa
     1681 ttttttgctg atagagtata atccttcacc agcttcaacc tgatatatat aatattcttt
     1741 tccatcaata atttttgtgg gtatttcgga tttctgtgca tatattattg cggaacagat
     1801 aattaaaagc acaagaagta atgtacttat tttgattctc ataattttca aaataacaat
     1861 aaaattatat tacagtttta gcgtttaaat tgcaaatgta caaaatttat agattggaga
     1921 ggctgtcaaa aataataaat cagccgtttt tttgtttttc aaaataaaaa agatgtattt
     1981 ttgtagaata aacgaaaaca ttacatcaaa attactgaga gttaattaac tcgttaaata
     2041 agtttacccg gtagtaagaa atccagccaa tcacagattt atatgaaaca tttttttctt
     2101 ttgttgatga tgtctgtcct tgctgtgaat acatattcac agatacaggt caagggcaat
     2161 tttacggtgt ataatgaagc aaccggactt gactatattt atattttttc aacgattgat
     2221 aatgccgctg agatacatta tacaaccacg gaaaatacaa atcaggtaaa atggtacact
     2281 ttcctgaatg gagtaaaaac tgaaatgacg agcgtatcaa ttgtttcgcc caccgagaca
     2341 tatgttgatc cgaagaataa taccggttac attatcaatg tgaacggaaa agatgtggtg
     2401 cgtttctggg tgttcgatta ttcgcaatat gtacccgcgt ttacttcaat aagtgcaaca
     2461 gacggtgaaa caccctgcga aaacatacag ttgaatttaa ccggtaattt accggaattt
     2521 aaatatcaga atacgacagg aggtatattc tccatcgacc gttattttga tgttgaatac
     2581 aacacacttg aatgggataa ggataannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnccaa acgtgcgaag ttatttctgg acaatatacc ggaatggtga aaagctgatg
     2761 gcacgttcgg agaaagatca cttatatact ttctcaaagg caggaaatta taaaattgaa
     2821 ctcaatgtta ccaatggaat ttgttccgat tcatcttcag taaatgtggt tgtaatggag
     2881 tcatcaataa ttgcaccggc ggtgtttact ccgaataacg atggttacaa cgaggagttc
     2941 agggtagctt acaggtcaat catagagttt cattgcacta tttacaatcg ctggggcagg
     3001 gtggtttaca cttcttctga tcctgcgaaa ggctggaatg gaacaatcgg aggaaagcag
     3061 gctgctgagg gaacctattt ttatatagtc aatgcgcgag gttcggacga taaagtctat
     3121 aaactgaaag gacatgtcaa cttattgaga tagaaaaagg cttcgaaaga agcctttttt
     3181 atgtctgata attattgatt taacagctta ttttacaatc actgcttcaa ggttcttatt
     3241 tctgatttga acatagacag gttctccgtt tttgaaataa ggagcattta cataacccat
     3301 tccgattcct tttttactgt cagggagcat ggtgcccgaa gtaacgtgac cgattttgac
     3361 accatcggtg tttacaatct cgtaaccatg acgtggaata cctctttcga tgagttcaaa
     3421 ccgtaccagt ttgcgtgttg ttccttcagc cttttgtttt tcgagcagcg cgcgatcggt
     3481 gaagtttttg ccttctgcaa atttggtaat ccagcccaga ccggcttcga tgggcgatgt
     3541 ggtgtcgtca atatcattgc cgtaaagaca gaagcctttt tcgagacgca gtgtatcacg
     3601 tgctccaagt ccgataggct tgataccaag gtccttacct gtttcaaaca gggcgttcca
     3661 tatatttaca ccatgttcgg gatagaaata aagttcaaaa ccaccagcac cagtataacc
     3721 tgtatttgag ataattacat gtggtactcc ggcaaaatca cctacagtaa aatgatagta
     3781 gggtacttcg gagagattaa cggaagtgag acgctgcaag agttctgttg ccttggggcc
     3841 ctgtaccgca agttgagcgg tatgttccga agcattttcg agaatggctc cttctgtgtt
     3901 gttatcgttc agccagttcc agtctttttc tacgttggaa gcatttacaa ccagcatgta
     3961 tttttcgggt tcgaaatagt aaatgataat atcatcgact atacctcctt ttccgttggg
     4021 gaagcaggaa tactgagctt gacccagcgt gagttttgag gcgtcgtttg aacacagttt
     4081 ctggattaaa tccagcgctt tgggaccttt cacccagatt tcgcccatat gcgaaacatc
     4141 aaaaacaccc acaccagtgc gtactgtgat atgttcgtcg ttgatgccgc tatattcgat
     4201 aggcatatta tagccggcaa attcatgcat tttagcacct aaagcaatgt gaatgtctgt
     4261 aaatggagta gttttcattg ttctgattac ccggttttaa atcaacggga gtgattttat
     4321 tatagttttt atttaaagtt attttactgg tttaacgttg tattgcaatt gttttatgct
     4381 gaaagtcatg attttttctc gagcagttcc acaatcttaa caatgaccat cattgctttt
     4441 tccatggacg gtatcggaat gtattcaaat cgtccgtgga agttatggcc gccggcaaag
     4501 atattcggac aggggaggcc ctcgaacgac aatctggctc cgtcggtacc acccctgatg
     4561 ggtttaacat tgggttttac atccaccgct ttcatggctt cgaacgcgag atcgaccaca
     4621 tgcataaccg gttccacttt ttcacgcata ttgtaatact gatcgcgcat ttctacaact
     4681 gccgtatttt cgccatactc tgcattgatt ttatttgcca ggtgttgtat ttccttttta
     4741 cggcgttcaa aacggtcacg gtcatgatcg cgaatgatat aactcagcaa tgttttttcc
     4801 actgtacctt ctatgttggt caggtgatag aagccttcgt aaccgtccgt atgttcgggt
     4861 gtttcgtgac gcggcaacat gctcacaaac tgtgtggcaa tgcgcatcga gtttttcatt
     4921 ttgtgttttg cgtatccggg gtgcacgtta aggccggtaa tggtaacctt agccgaagcg
     4981 gcattgaagt tttcaaattc cagttcgcca atttcgcctc cgtccatggt atatgcccat
     5041 tccgcatcaa attttttcac atcgaaatga tcggcaccct gaccaatttc ttcgtcgggt
     5101 gtaaatccga tgcgaatttt tccatgcttg atttcgggat gttcagtgag gtactccata
     5161 gctgtaacta tttcggccaa tcccgctttg tcatcggctc ccagcagggt tgttccatct
     5221 gtgacaatga tgtcctgacc tttgtactgg agtatttcgg ggtatttatc agtttcgaag
     5281 agaataagtt tttcctcatt gagcagtata tcgttcccat cgtagttttc gataacgcgt
     5341 ggtttcacat ttttaccgct ggcgtcgggg ctggtgtcca tgtgtgaaat aaagccaatt
     5401 acaggcacat ctttgtcggt attggcaggt aaggtcgcca ttacataccc gtttttatcc
     5461 agggtaattt ctttcatgcc aatggatttc aactcgtcaa cgaggtattt ggcaaaaatc
     5521 atttgtcctg gtgtgcttgg tgtcagatta gttttttcgt ctgaagtagt tgtgaaactt
     5581 acatacttca aaaaacggtc ggtaatattc atgaatatat tgaatttaaa attagtgtac
     5641 aaaattatga attttatctg tattaaaacc ggaatatctt aattttgttt attcaggtaa
     5701 tgaaaaaaat gagtttaatc tttcatttta aaattgtctc ggaggcaaag gtacgagtaa
     5761 ttccattact gtaatgtaca gacggataac cgaatatgac aaacaatcct tcgcggcttt
     5821 tatatcttat cagatatttt tctctgagtt tttcggcttt cttcccgtac tggataaacg
     5881 gatgaacact gccaatcata caggtaccca ggcccagaga ttcagcagtg accatcgcgt
     5941 aggtggcagc aataatcgga tcggcgggat cgcagtaagg agaaccgtag aagtacatga
     6001 caagaggtgc tccgtaagtt atccagtttt taccttcttc gttgttatta ataaaaaaat
     6061 caaaaagcgg actgataaat gatttgtata actccgcgct ttctctgctt ataaacggac
     6121 gcatcaacgc ctgtacaggt ttggaagcaa accatttcat gcttttcagc atttccgaga
     6181 agtcttttac aaattcggta tttatctctt ttccatttaa tatcagcaca tttacgtcgg
     6241 taggtggcaa gcccattggc gccgtacggg ctgctgccag aattttttct ataatttcat
     6301 cttcaaccgg ctgatcccgg aactcacgga tacttcttct gcgctgtagc agggacagaa
     6361 gatgatcgta tgccgcgaca gaatttgccg ggggcagttc gaacaaatca tcgggcgata
     6421 aggttctgcc ggtaattttt attgctcctt tggggcaaat tgccatacaa tgaccacagc
     6481 tgatgcagcc gaaaaccggt gtaccgctga tgacagcctt tccgttttcg atgattaaac
     6541 tgaaatcctt gcacacggaa acacacaatc cacatccatt gcataactca ttgtcgatgc
     6601 tgatgatgcc ttcttctttg gttcgggttg ttggtatagc cataaattga aatttgaaaa
     6661 aatatgtatc ttatttactg atatctttga tattaattca agtgatagcc ttgttcgagt
     6721 agttcaagtg caattaaatg ccggtgcttt tcaacatcca gcggataatc ccaacaacca
     6781 agactatgat agatttcacc cccgaagccg gttttgaagc ggtaaagtcc atacaacggg
     6841 tgatttggat ctgggtttgg tgaaacgcca aacatgtcat attcggtaca gccgctcgct
     6901 ttagaaattt ttatggcttc ccactggagg gcgtatgtag ccatcagatt tctgttttct
     6961 gatgatgacg caccataaag ataggtcccc cggttgccgg taataatgag aaacatggct
     7021 gccagcggct gattgttata ttcagcaata agcaatttta cttcggcggg cgaacgcatg
     7081 tcatttgttt tggcagatag tacagccctg aagtatttaa tatcattggt taagatatgg
     7141 tttctttggg cagtttcctg atacaattta taccagatgt caatattttc gatacccgac
     7201 gattttacaa ctattcctct tctttcggct aatccgatgt tgtatctggt tttgggtttc
     7261 atcgtagcca gaatttcatc aatatcctta ttcaaatcga cgtaaatggt atttgacggt
     7321 aaaatattgg acgatgtttt tttaaagttc cagttgacgg tattaaaatt gaatcgcatt
     7381 tcctgcgtcg atatttgggg ttcacctatc cagttaccgt caggatcgaa ataattctgc
     7441 tctttggccc aataggactc ccagcacaaa tcatatctta tcatgatgca atttttcggg
     7501 aggaaggatc tgatgcattc cgacaactct tcaagaaaca atccctgaaa ttcgtcatcc
     7561 ggttgcagtt caggtccgta aggcacataa gcaatcgaat ggttttgatc aatatatctg
     7621 actattacca gcacatcaga tacaattaca tcatcctctg actgtggaat gttgtataag
     7681 tccgattttc tggagttgaa attaatagca agagtcgttg ccccgagttt tttcttgacc
     7741 acggaccaaa acgccgtttg ttgaacaatc ggagtagtat ataattgttc aatttcctta
     7801 ttttgtatat atgtaagcat gattattgca aaggtaacat gtaaaaatga atataaaaaa
     7861 ttaattgcca gataaaagat tttcagaagt ttaattcatg gctcagatta tttacctgac
     7921 caatttcagg aacctattca caaaaggaga taagaatcat ttcttaccaa atattttatc
     7981 cgcggtaaat ttaaatttaa tatccattaa aacacgaaaa atttattaag tttgtagctc
     8041 attattattc aattatgcgt catagtatca tcaccatact gatattgttt tgttttattc
     8101 cattcatttc cggtaacgcg acgcttccac tggacagtgt actgtatcgt accatgaacg
     8161 cggctgagac ttacagcaga cttatcagcg actatgaggc tgaagtgtat atgctcgctt
     8221 atgtaaaaac acataaaaag aatttcctct ataaatatac ctatcttgtt ccgagatttg
     8281 tactgcatga ccctaaaaat gatgaaggcc tgatagaatc tgtcagtaaa ctgaaaatga
     8341 gttcttccaa tatctactat caggatataa atctcatcaa cggaactatg acccgcaaga
     8401 gtgaaatagg catgcttccc tttaagttta tgagtgtaaa tgtgtataag gaagccgcgc
     8461 ccgatgaaag ttatctactc ccgctgcgga aaagttccgc gaagtattac cggtataagt
     8521 tgctaagctg cgattccctc aaacataaac ctgaatatac gataggtttc acaccccggt
     8581 acaagaattc gaaacttatc agcggttcct ttgtaataga agaaaacacc tggcgtgtaa
     8641 agcaatttat tggtaaggga agtgatcttt ttctccggtt tacatttaat atcactatgg
     8701 gcgatgatgc caataatcag tatctgcctg tgaattttac aattaaccag tcctatgtat
     8761 attttgggaa caaggttaca aacaggtata atgccttact gacctataaa aatgtaaatt
     8821 tcaacggggc aaaagatcac ctgaataatc ttaatctggg taatcgatat aaaattcgtt
     8881 tggattcagt tccggttaat attgacagcg cctactggaa caaaatccgt cctgttccgc
     8941 tttccgacaa tgaaaaaaac ctgatggcta aatacctgga gaaacgaaga attattcaat
     9001 cgcagaaaaa agaggtggat actgttgctt taaaaacgag aaacgcgttg acatttgcaa
     9061 aaaacatggt aatgaacacc aaatacagtt ataaatccac taatatccgg tacagtggca
     9121 tccttaatcc ttccatgata agctattcaa ctgaagatgg gcttacctac cgtcagaaat
     9181 tgtaccttgg aatagaactg gaacggcatc agcggataaa tattaatgct tttgcaggct
     9241 atgttttcaa aaaaaaggaa ttttacggag atttgtccac tgtatggaat tatgatcccg
     9301 ccaagtatgg aaatctttca ttctccatcg ggaaaggtaa tcagtcctat agttcgctgt
     9361 ttctgaaaat ggtacaggat agtctgacaa gtcaggggct gaagtttacc gatttgggtg
     9421 tcaactattt taaggattac tatatgcgga ttttcaacag tgtggaaatc agtaatgggt
     9481 tgcagctggg tgcgggtttt gattatcaca tccgtgaacc aaacaagaaa caaatgaacc
     9541 ttactgcccc aaaccatgat cagatggata atatgttcga taccaggtat tattttgtgc
     9601 caatcatcag tatgagctgg acacccgagc agtattatac gatggataaa aatcagaaag
     9661 tatatgtccg ttcagattat ccgactttca agatacagta tgctcacagc ctgaagaata
     9721 tattcggaag cacttcgcaa tacagccggg ttgaatttga tatcaatcag aatattcgac
     9781 tcgatctgat gaaatccatt caatatcatc ttggtaccgg attcttttcc cgccagaaaa
     9841 ctgagtattt taccgatttt gtctatttcc gccgaagcta tttccccgaa acatggggcg
     9901 aagggatagg aggggttttt aatgttcttc cccgaagctt gtataatgcc tcatcgtcat
     9961 acgttcaggc tcatttgatg tacgaaacgc cacgatttct ttttacccgg atacccggct
    10021 tgtcgggtgg tgttgtaaag gaaaggcttt atttcagcca gctatatacg cctacaattc
    10081 cgtcctatag tgaactgggt tacggtgtgg gaaaccggtt tctgaacgcg gccatttttg
    10141 tgtcattctt taagctgaaa taccaggaca tgggctttaa aactgtattt ttactttaat
    10201 ccggatttag ctgaaagaag gctcattatt ttttgcttca tcaatccggc cgctgttgaa
    10261 taatccggtt atttcggtaa acgccaacat attcctatct ttgcagccgt aaaaaagata
    10321 aaacagtaaa tgcagaaaac atatctacta accgattttt tacccaccac aaaaaaagaa
    10381 ctcgaattac gcggttggga tgaagtggat gttatccttt tcagcggaga cgcttatgta
    10441 gatcatccct cgtttggggc ggcggtcatc ggtcgtatgc ttgagttcga aggacttcgg
    10501 gtggcgttgg ttccacagcc caactggcag gatgatttgc gtgatttccg aaaactggga
    10561 cgaccccgaa tgttttttgc ggtgagcgcg ggaaatatgg atagtatggt aaaccattac
    10621 acggctaata agcggatgcg ttcagatgac gcgtattcgc ccgatggccg tgcacacatg
    10681 cgccccgatt atgccacaat aacctactgt aatatactga aaaaactcta tcccgatgtg
    10741 ccggttgtga taggaggcat cgaagcctct ctgcgcaggg ttacacacta cgattattgg
    10801 tcggataagc tgatgccctc cattctcatc gacagcaaag ccgattttct gatatatggt
    10861 atgggtgaga agccacttaa agcattgctt gaagaactga aaaacggtaa aaaattcggt
    10921 gaaataacag atattcctca aacctctttt ctgtcagaca agattcccgg ggacgataat
    10981 cttcaaacca ttgagttggt ttcacacgag gaatgcctga aggataaaaa gaagtatgct
    11041 tccaacttcc gttatgtgga agaggaaagc aatcggatga atggtgcacg tctggttcag
    11101 aaaacacgtg gtaagtacct tataatcaat ccgatgtacc ctccctggaa accggaagag
    11161 ctggatgcaa cttatcggtt gactttcact cgtttacctc accccaagta caagggaaag
    11221 caaattccgg cttatgaaat gatcaaattt tcggtcaatc tacatcgggg ttgctttggc
    11281 ggctgtgcat tttgtactat ttcggcccat cagggtaaat ttatcgtttc ccgaagcaag
    11341 cagtccattt tggaggaagt cagacaaatt gctcagcagc ccgatttcaa aggcaatctg
    11401 agtgacctgg gcggaccgtc ggccaacatg tacaacctgc atggtatcga tatgaaaata
    11461 tgcgaaaaat gcaaacgtcc atcgtgtatc catccgaaag tgtgtcccaa cctgaatatc
    11521 aatcattcag ctctgaccga tatttaccgt tcagttgata aaataccggg cattcgtcat
    11581 tcttatatag ggagcggtat ccggtacgat atgcttactg cggaaacaaa aaatgctgaa
    11641 acaacaaaaa gtcagcttga ttatatgcgc gaacttgttt caaaacatgt ttcaggaagg
    11701 ctgaaagttg cccctgaaca cacttccgaa cgtgtcctga aactcatgcg taagcaaagt
    11761 tttcaggatt tcaaaaaatt caaggtttta ttcgaccgta tcaacagaga ggaaggactt
    11821 aatcagcagt taatacctta ttttatatcc agtcatccag cctgtatgaa tgaagatatg
    11881 gccgaactgg cagccattac caaggacttg aatttcaaat tggaacagat acaggatttt
    11941 accccgacac ccatgactct tgccacagaa atatactatt caggctttaa tccttatacc
    12001 ctggagccgg ttttcacagc taaatctaag gatgaaaaac tggaacagcg aaaattcttt
    12061 ttctggtaca agaaagagta tcatcaacag attatgcgtg atttgcaaaa aatgaaccgg
    12121 caggatttga taaagaaatt gtttcaataa acggtgcaat gatgctgtaa attgctggta
    12181 atgtttctga gatgttttcg ttaccgaatg atttcaccat tcactatttc gaaaatctgt
    12241 gaactctgac caatctgtgc cagaatttcg tccagatgtt tccggttagt gtctgtaata
    12301 aaaatctgac caaagttatc accggcaacc agttcnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnncggtc gcgttcacgg ttttgtttaa gtttcaggct taaatccgct
    12481 tctgctaatt gcgatttgta gtttaagctt acctgttcgt taccacagga aataaaccgg
    12541 tatatttcct gaaaaatagg aataaattca tcaataaaag atttccggca ttcataaata
    12601 tacttaccgt gtttaaccat cagttcttcc catatttcga aaagagtctc atcgaataaa
    12661 gtctcttctt tgagtagtgc gtttcgttgt ttcagggcat tgttataatt cagaagcgcg
    12721 ttcagataag ttttattata ttgcgaaatt acactgtcca tgaattttcg tcgctcatca
    12781 ctaccctccg aaataagttc atcatcgccc ggcgagacca aaaccaatgg cagcaatcca
    12841 atgtgatcag ccagtcgttc gtattccttt ttgttgcgtt tgaaatgctt cttttgtttc
    12901 cgtttcatgc cgcagtatat ttcctcttca gtatcccgaa gtgagtattt gccctgcaac
    12961 ataaaaaagt cagcattatg ctcaatattc tgcgagtcaa tggaattgga ataactttta
    13021 caaaacgaaa gatagtatat tgcgtcaagg aggtttgttt ttcccattcc attgttccca
    13081 acgaaacaat tgattttagc cggaaaatca agatctgcct gacaaatatt tttataattc
    13141 agtatggaga tggaattcag acgcataatt tggtttaatc aattactcac agcttttcgg
    13201 aaaaataaca atgcaaaaat agaaaaaaaa cgctaatttt gtctctttat ccattgtatt
    13261 gcagtgtaaa taacgttatc tgaatggaat aattcatgtt ttttatcacg aaatgtacac
    13321 ttaatacatt atacagtttt tatactaaaa atttttattg acaaaaaatg atatcagacc
    13381 aacttattaa acttctttcc gcttcctgtc agcttagtac ccgtcaaact ctgaatgcgc
    13441 tcaaattact tgaagaaggg tcaaccatac ccttcatcag tcgctaccgc aaggaagcga
    13501 ccggaggcat ggatgaagtg caactggctg atttgaaaga acagtatgat aaactttgtg
    13561 aactcgctaa gcgaaaggct acagtcctca aatctatcga ggaacaggac aaactgacac
    13621 ccgaacttca gaaaagaatt gaggaatgct ggaattcaag tgaactggaa gatatttatc
    13681 tacctttcaa gcccaaaaga cgtacccgtg ctgaaattgc ccgtgaaaaa ggactggaac
    13741 cgcttgcgaa aataatcatg aagcagcaat cagatgatat tgaatcagtt gcaggacgtt
    13801 ttataagtaa agatgtggca gatactgaag tagcattgaa aggagcccgc gacattattg
    13861 ccgagtgggt gagtgaaagt gaaattgcca gaaatttaat tcgtaaacaa tttgcatacg
    13921 aggcaaatat atcatcccgg ttgataaaag ggaaagagga ggaagcacag aaattccgcg
    13981 attatttcga atgctctgaa aaacttgcac gttgttcgtc acaccgcctt atggcaatgc
    14041 gcaggggtga agctgaaggt ttccttcggg taaacatttc agccgatgat gacaaatgta
    14101 tcactaaatt attgagaatt ttccagaaat cagataatga atgtggactg caagtagcag
    14161 aagcggtgga agatggatat aaacgtctgc taaaacctgc catagaaact gagtttgccg
    14221 ccgaaagcaa agagaaagcc gaccaggaag caatacgtgt ttttaccgaa aatttacgtc
    14281 agctgctcct ggcaccacct ttgggacaaa aaagagtaat gggagtcgat cccggatttc
    14341 gtacaggttg caaaattgta tgcctggatg aacagggaaa cctgaaacac aatgagaata
    14401 tatatcccca tcctccggtt aatgagcatt cgcaggctat gcgaaaactt caaaaaatgg
    14461 tagaagcgta tgatatacag gctatagcaa ttggaaatgg aactgctagc cgtgaaacgg
    14521 aacagtttat tcagaacatt cgcttcgacc gtaaggttca ggtatttgtg gtaagtgaaa
    14581 acggagcttc gatttattcc gcttccaaag tggcacgtga agaatttccg gaatatgacg
    14641 tgaccgtacg cggagctgta tctattggtc gccgactgat ggatccgctg gccgaacttg
    14701 tgaaaataga accaaaatcc attggtgtag gacaatatca gcacgatgtg gatcaggtca
    14761 aactcaaaaa atcgctcgac cagacagttg aaaatgctgt aaatagcgtc ggagttaacc
    14821 tgaatacagc aagcaagcat ttgcttacct atgtttccgg attgggtcca caactggctc
    14881 aaaacatagt gaattacaga gccgaaaacg gttctttcac taaccggcag gaacttttga
    14941 aggtgacgaa aatgggtgca aaaaccttcg aacaatgtgc cggttttttg cggatagcaa
    15001 atggcagtaa tccgctcgac aattcggctg tacacccaga gagctatagt attgtaaatc
    15061 agtttgcaac cgacttaaaa atggatatta atcaactgat aggaaataat agcctgaaat
    15121 caacgttaga tttaacaaag tatgtgaccg atacagttgg actgcccact ttgaacgata
    15181 ttctggaaga acttgagaaa ccgggaaggg atccgcgttc tgcaataaag gtatttgagt
    15241 ttgatcccga cctgaaggat ataaaggatt tgcgaaccgg catggaatta ccgggaataa
    15301 ttactaacgt aaccaatttt ggggcttttt gtgatattgg tatcaaagaa aacggattaa
    15361 tccatatttc aaacctgtca gaccgttttg tgagtaatcc ggctgatata gtatccgtac
    15421 atcagcatgt cagggtcagt gtaatcgatg ttgatatgac ccgaaaacgt attcagttga
    15481 agttattatg aatatattag aaaaatcaga aatgaaaaaa acgataagtt gtctgatctt
    15541 attattgatc ggtttttctg gctttactct gaaagctgaa gaaaatgccg tcattaatgc
    15601 agagcagact atgatcaatg aaagtctgca gcttaataaa aattccattt tgttttccag
    15661 tcttgtttgg gataagaata aaatcacatt gaaaaaaaat aaatcggatt ttggtaagca
    15721 actctggaaa aatgcaaatt ttggctggaa tacgtataaa acagatgatt ataaatggag
    15781 aaatatatta atcggaaccg gatatggcag aaactttgag catgtcaaga aagcttattg
    15841 gatggttgga ctgaacctga actggtcgaa atacacactt tatcccgacg gagcattcaa
    15901 ttgcacagcc aataaagatt atcttaaaac cacttcattg tccgttccgg ttactgcggg
    15961 atacactatt tatacttcca aacttaggtc gatgaatcta aaagctttta caggaccggt
    16021 ttttgaatat attttgacgt caaaattaaa cggttacaca gataatctgc atcctatcaa
    16081 caattttcaa acaggctggt cggcgggtgc caattttcgg ttcttatacc tgctgagtgc
    16141 ccgtgttact tatacatatt acccaaacag cttatttgaa aatcatgacc tgtatcgacg
    16201 ttcattaagt ttctctttag gtttttaaaa accagctttt cccgtgttcg taacttagct
    16261 tcaatccttc atccctaaat tgagttttta atgtgtgcga ttaaaaataa tttaagtact
    16321 tttgcgtact tcaaatcaaa acttaaatca gatgttacaa tcaatgaccg gctacggtaa
    16381 agccagctgt gaaattaaca acaagaaaat tgttgtagaa ataaagtcat taaacagtaa
    16441 gcagttagat ctttctaccc gcattgccgg tatttaccgg gaaaaagata ttgaaatccg
    16501 caatcttatc tcacaaaaac ttgaacgtgg taaagtggat ttttcgttgt atatcgataa
    16561 ttccggaaaa gacagtggtt cgaaaattaa tcaggcggta ataaaatctt attatgaaca
    16621 actgaaagca ctttcaggtg atttggaaat acccgaatca actgactgga tgtcaatatt
    16681 gatgaaaatg cctgacgcgt taaaaaccga aaccgaagag cttgatgaaa atgagtggat
    16741 cgagattctg aaatctatcg aaacggctat taatcagtta attgaatatc gcagacaaga
    16801 ggggaaaaca ctggaaaatg ttttcacact gaaaataggc agaatagggg agtttttggt
    16861 cgagatagag ccttttgaat ctgaacgggt tgataaaatt aaacaacggc tggatgaaaa
    16921 ccttgctgcg ctatcggata aaattgatta tgataaaaac agactggagc aggaattgat
    16981 tttctatatc gagaagctgg atgtaaatga agaaaaagtc cggttgaaaa atcatctcga
    17041 atacttcatc aaaacaatga aaaatgaacc atcaccggga aaaaaacttg gttttattgc
    17101 gcaggaaatt ggtcgcgagg taaataccct tggttcaaag tccaatcaca gcgaaatgca
    17161 gaaaatagtg gtgaagatga aagatgaact cgagcaaatc aaagaacagg tactgaatac
    17221 cctttaattc tttttcttaa tgtatatttt cgctctataa tttaagttgt tctgtatgca
    17281 gggtaaactg attattttct cagcgccttc cggtgcagga aaaagtacta ttgtaaatta
    17341 tctgctatcg cgcggactga atatggaatt ttccatatcg gctacctcgc gtgcacccag
    17401 aggcaacgag cagtatgggc atgaatacta ttttatatca acggaagaat tcaaagaacg
    17461 tatttcaaaa ggagaatttg ttgaatatga ggaggtttat tcaggttgtt tttacggcac
    17521 tctccacaat gaagtagaac gcataacttc atcgggccga aatgtaattt ttgatgtgga
    17581 cgtgaaaggt ggtttgaata taaaaaaaca atttggagac cgtgcattgg ctgtttttat
    17641 tgcaccgccg tcaatagaaa cacttcggca gcgccttata aaacggagca ccgacagccg
    17701 ggaaatgatt gaaaaaaggg tagggaaggc tgcttacgaa atgacctttg cgcctcagtt
    17761 tgatgtggtc attctgaatg ataaccttga aacagcaaaa atggaagccg aaaaggttat
    17821 aaaagaattt tttgaaagat aatcatgtat aaacacatca gaaacaatta gttatgaaat
    17881 ctatcggttt attttttggc tcattcaatc ctgtacatct tggacatacc aatctggctg
    17941 aatatatttt tcgtttttca ggtgttgatg agatttggtt tatcgttagt ccccgaaatc
    18001 cgctgaagga acagtctgag ttgattgatg aacatctgag actgaaaatg ttgcagctgg
    18061 caaccgccga aacagaatac ctgatagctt ccgatattga gttttacctc acaaaacctt
    18121 catacaccat caatacgctg aaaacgctaa gcgaaaaata tccagaagat aatttcattt
    18181 tgttgattgg cagcgataat atgcagattt tcgatcagtg gaaagattat cagacaattc
    18241 tggatgagta ttcagtggtt gtgtatccac gcgaaggtta tccctacgag gactttgaag
    18301 aaatttatcc cgatatgcaa atactggaag aagctccgct ttttgagatt tcttcaactg
    18361 aattacgtca attgatagct gaaaaaaagg atgcaagcca atggttgcat cctgatgtat
    18421 atcagtttat cctcgaaaac ggtttgtatc tgtcctgaaa aaaatatctc agactcatta
    18481 cagaaatgga ttgttttttt tctcatttcc gatggttgtt tttggtccgt gtccgctgta
    18541 aaccaccgtg ttgtcgggca atgtaagcag tttttcctgt atggaacgaa ttagtgttga
    18601 ataatcacct ctgcccaaat cagttcttcc aatcgatccg ttgaacaagg catctcctgt
    18661 gaaaacaatt ccctctttct gattatagaa tgccagactg cccggtgagt gacccggcac
    18721 tgacagacat atcatactgc tgtttccaaa ctttatttca tcaccgtcag taaggtattt
    18781 gcctaaaggt tgtacagctt ctttataggg cagaccgaac atctgagccg attggtgtgc
    18841 tgttgcaagc agaaattcat cttcttcact ggcttcgggg gcaatagcga aagtatcaaa
    18901 cagaaacttg tttccaaatt gatgatcaag atgtaagtga gtgttaatca cttttataag
    18961 agataatttg tgcgtttcca tatacttaac gatttccgct ttttcccttt cataaaacat
    19021 gccagcatcg attatgatcg attcaccggt ttcgtcatac gccagatatg tgttttcctg
    19081 aaatggactg aatgtgaatg ttttaactgt cataactata agtcgggatt ttacagcggc
    19141 acataaacca ctttcttggt tttgaaaaat tcaccattaa attgatcgct cagttcgtag
    19201 gtttcagcaa agtttttata ggggagtatt tcgtgctgta gttcgcctcc tttcagacat
    19261 atcaaaccat tggggagcgc attttgttgt ttagtgcaaa tattttttct gaccagctta
    19321 accaaatcat caagcggcat cacagcccgg cttaccacaa agtcgaattt gtttttttcc
    19381 tcttgtacac gttcatgctt taatgtgatg ttgtttagtc caatagactt tgaaacttcg
    19441 cttccaactt taattttttt gcctatgctg tcgatgagtg tgaaccgtac ctgcggaaac
    19501 aatattgcca gtggaatacc aggaaaacct cctccggttc caacatcaag aattgaagaa
    19561 cccggtttga agctaatcac tccggcaata cccagcgaat gcaaaatatg tcgctcgtac
    19621 aggttatcaa tatctttacg tgatatgaca ttgatttttg agttccagtc cagatataaa
    19681 tcaaacaatg ccttatactg atttttctgc gtttcggtca gatttgtaaa gtattttaga
    19741 atttcatcca tattatcttg attatagaat accgagtgaa agattgtttc gaaattcaaa
    19801 agtacttgtt tttttagaaa taataaaatc agattttgtt actgtcaggg tacatgattt
    19861 caatattgtt cagataaata aaaatgtata actttgcaac ccgaaaaaaa caaattaaat
    19921 aacgaaattt acaaactaca ataaaaaatt atgaaagcat ttgtatttcc cggacagggc
    19981 gctcagttta caggaatggg taaagacctg tacgaaaaat cgcctgtagc aaaagaatat
    20041 tttgaaaaag caaatgatat acttggattt cggattaccg atttgatgtt tgaaggcact
    20101 gctgaagatc tccgtcagac aaaagtaact caaccggcag tttttcttca ctcggtaatc
    20161 tcagccatcg cattggccaa tgaatttaat cccgatatgg tagccggaca ctcgctgggc
    20221 gaattttctg cactcgttgc tgccaaagca ctcacattcg aagatggtct gaaactggtt
    20281 tatgcacgtg caatggcaat gcaaaaagct tgcgaagctg aaccatcaac tatggctgct
    20341 gtactgggtc tcgatgatga aacagttgag gaagtatgcg atagtattaa ggaaaatgtt
    20401 gttgttcctg ccaactataa ttgtccggga caaattgtaa tttccggttc gatagaaggt
    20461 gtggatcagg cttgtgagaa gttaaaggaa aaaggcgcta aacgtgcttt aaaattaccg
    20521 gtaggcggtg catttcactc accgctgatg caacctgcca gtgaagaact cagagcagct
    20581 attgatgcag ccattgtcag cgaaccgata tgccctgttt atcagaatgt taatgcctat
    20641 cctcaaacag atcccgtagc catcaaacaa aacctgatag cccagcttac agctccggta
    20701 agatggacac agacggtaaa gaatatgatt acagatggtg ctgttgaatt tgtagaactc
    20761 ggtcctggcg atgtactcaa aggattgatt aaaaaagtaa atccagatgt tactgtagga
    20821 taattttatc gagaaaaaag tttttttgaa aatattcagg cacaaaaatt taattatttg
    20881 tgcctgatat tgtttaaata aatacagaat aatcgatttc ccagtttttt tcgtactttt
    20941 gtatgtacaa tttaatcaga aaaacactta aaccatggat actcagttat tgaggaataa
    21001 ggaattgtac ccaactgaag aagtgcttca aaaagtactg atggatagta ttcaggctta
    21061 taatcagctg gttacaacta ttgctgagct gcaggctgaa atgatatgga ggtattacaa
    21121 tgacggaaaa tcctggcttt gtaaggttca gcataaaaac aaaaccattt tctggttatc
    21181 tgtctgggac ggttatttca aaacaacttt ttattttaca gaaaaaaact gccagggaat
    21241 tgaactgctt gatattgacc aaactatcag gaatgaattc agaaataata aacccatcgg
    21301 aaagttttta ccactgactt tgacaataca aaatagaaaa caactgattg atttactgaa
    21361 aattgcagaa tataaaagag gattaaaata atatagctat taattttatg tttaggacaa
    21421 aaacatgtgg agaactcagg cttgccgatg caggtaaaaa agtaaccctt gccggatggg
    21481 ttcagcgcag ccgcaaaatg ggcggtatga ctttcattga tttgcgtgac cgttatggaa
    21541 ttacgcaatt agtatttaat caggatgtta atgctgaatt gtgtgaccgg gccaataaac
    21601 ttggacgaga atatgtcatt caaatctgtg gcgaagtggc agaacgaagt aataaaaata
    21661 cccacatcgc aaccggtgaa gtggaaatac ttgtagatat tctcaacgta ttgaacgagg
    21721 caaatacacc accatttacc atcgaagata atacagacgg gggcgatgat atccgtatga
    21781 aatacagata cctggacctg cgccggaata gtgtaagagc aaatctggag ttaagacata
    21841 aaatgaccat cgaaaccaga agttacctcg acaaactgaa ctttcttgaa gtggaaacac
    21901 ctgttctgat tggttctaca cccgaagggg cacgcgactt tgttgtacct tcgcgcatga
    21961 atccgggtga attttatgca ttaccacaat caccgcaatt gttcaaacag ttgttgatgg
    22021 tttcgggctt cgaccgatat tttcagattg taaagtgctt ccgcgatgaa gatttacgtg
    22081 ccgaccgtca gccggaattt acccagatcg actgtgaaat gtcattcgta cagcaggaag
    22141 atgtgctgaa taccttcgaa ggactggcca aacatctttt caaaacgata aagggaattg
    22201 aaatgaacga acctttcccg agaatgacct ggcacgatgc aatgaagtat tatggttccg
    22261 acaaaccgga tttacgtttc ggaatgaagt ttgttgagct taaagaagcc gttacaggtc
    22321 atgatttcag tgtttttgat gaagcagagt atgtgggcgg catctgtgca agcggttgcg
    22381 catcgtacac ccgcaagcaa ctggatgaac tgactgattt tgtaaaacgt ccgcagatag
    22441 gagcgaaggg actgatttat atccgcatga atgaagacgg aacggtcaaa tcatctgttg
    22501 ataagtattt taatgaagaa aacctgaaaa atattgccgc caaattcgag gctaaagtcg
    22561 gtgatttaat tttgattctt tcagggccaa aacgtaaaac gcagaaagca ttgtgtgaat
    22621 tacgtctgga gatgggagag aggcttggtt tgagagacaa acaggttttt gcacccctct
    22681 gggttatcga tttcccgtta tttgaatggg atgaggaagt tcagcgcttt tttgcgatgc
    22741 atcatccttt cacatcacca aaaccggaag atattcactt actcgacagc gatacaggta
    22801 acgtacgcgc caatgcttat gatatggtca tcaacggggt agaacttggt ggaggatcca
    22861 ttcgtataca cgacagtcag ttgcaacgca aaatgtttga attacttgga tttacacccg
    22921 aaaaagctca ggctcagttc ggatttttga tgggagcctt tcagtacggt gcacccccgc
    22981 atggtggtct ggcattcgga cttgaccgtt ttgtatcatt atttgcaggt ctggatagta
    23041 tccgtgattg tattgctttc ccgaaaaata actccggtcg cgatatgatg attgatgcac
    23101 cgtcgaaaat tgatcaaagc cagctcgatg aattgaatct tattgttgat ataaaagaat
    23161 aagtaaaata tattttcaaa taaaaaatcg cagagaagaa ttttctctgc gattttttat
    23221 gtttcaaatt ttgaaaggat tcataactcc aaaccttttc aaacttgaaa taatatttct
    23281 gaaaaatcgg aggtacataa ttacagtaaa ttctatttta cataatataa attataggaa
    23341 aatgacatca attttaattt tgccaggttt attgtaattt atgtcaattt tccatgaaaa
    23401 caactttcgc tgagaattca atattttcca ttcgattagt attacggtca gtttatcaaa
    23461 atctcagaat atctgcactt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23581 ctttacctgt atgtcaggtt tcttataaaa aatattatat tattggaaat aaaatatata
    23641 caaatatatt tcattaaaat agggtagtcc cctactcgac ttttcagatt tttattgcaa
    23701 atttctttaa tcgctgatca agcatatgtt gaggaataat ttgcttaatt gtctctgcta
    23761 cagcatttaa ctgtccttcc cggataaaat cctgatgaat aatcaatgaa atttctcgca
    23821 caggttccgg atttatcagc attcgaaggt tttttttctg attttcgcta agcgaattgg
    23881 tatgaagctc cggaataaca gtatagccgc cattttcgtc aacgatttta ataagcgtgt
    23941 caatacttcc ggcttcgtaa atattgcttg caggtgaaag tttacaaaaa ttaaaaacct
    24001 gacttctgaa acaatgtcct tcctccaaaa tccataagtt ttcaattcgt aaatcattgg
    24061 aattcagttc actgcatcta tataattcat catctggcga aatataggca acgaattttt
    24121 cataatatag tggtatttcg agtattagag gattatttaa gggcgtggat gcgatcgcca
    24181 tatcaatttc tcctaacaca agtttttttt caagaactga tgtatgcatt tcggatacct
    24241 tgaatgacaa atcgggatat ttcttattga agtgtacaaa aaactgcggc aacaaatatg
    24301 gcgcaactgt tggaatgaca gcaatattaa aatttccggt taagttacct ttttcattag
    24361 aaaccacttc tttgatccgg ccaatctgac agactgtact ttgagccaga tcaattactt
    24421 ttttgccaat tggagttact tcaatcggat gttttgaacg atcaaatatc tttacatcaa
    24481 gttcatcttc aagcttttgt atcatcatac tcaaagtagg ttgtgtaaca cctgcagatt
    24541 cagctgcctg tacaaaatgg cgcgttttat tcagcgcaat aatgtactca agttgttgta
    24601 gagtcattct attgttgttt atatttacac aaagatataa atatacttta tattactata
    24661 atttattttc caaccactaa aaaatatcaa attatagata ctaaaattga tattgttaaa
    24721 atttcagtta tttttgctga aaaaaataat gaagaaatat aatcttttta tactgatact
    24781 tatattcagt ttgaatgcct cgcaggcaca gctgttgtgg aaaatcagcg gtcatggcct
    24841 tgaaaaacct tcatatctct ttggtacaca tcaccttatt cctattcagt ttttggatag
    24901 tgttcccgga ttatacaaag cgtttaattc tactcaaact gttgtttccg aagtggtgct
    24961 taacaatctt gatacagcgc ctgccataca acgagcggcg ttacttcccg attcaattac
    25021 cattcaaact ttacttagca agactgatta tgactttgca aatgaagaga ttacaaagac
    25081 actccgaatg agcctcaata gtcttaacaa gatgcatcct tcgctcattg caaagtttta
    25141 tgaattagaa ctttataaaa cagttgagca ttttgatgag aatacgaagt ctgattccta
    25201 ttttcagctt gtagcagcac agaaaggtat tccggttgtc ggactggaga cagtagaaaa
    25261 gcaactcaac ttgctttttc caaaaaacta ccaaaaagag gcacaattgc ttattgaaac
    25321 tatcagaaac aaagataaac ttattggcga aattaatgaa atgaaccaga tgtaccgcaa
    25381 agcgaaaatc tatgaattag aaaaattggc atcacaatcg aataaacgat ggaatgtgag
    25441 tgaagaagaa aattatgttc ttgttgacga acgaaatatt gactggatca ggcaattgcc
    25501 cgaattacta aataaaagca gttgttttat agcagttgga gttttacacc tgcccggtac
    25561 taatggtctg ataaatttgt taaaaaaaga aggttataaa gtgacagcta taaattgata
    25621 aaaattcggt gaagcattca aaattaaatc aaattaatcg caataatctt tttttaatta
    25681 caatttaaat tataattttg caattcagtt aaagtcgata aaaacaacag aataaatgag
    25741 taaaacaaag aaatttcttc tgcctgaagc agagactcct acccaatggt acaatatttt
    25801 ggcagatatg cccaataaac caaaaccaat tctaaatccc ggaacaaaac aacctttaaa
    25861 gccagaagat ctttatccga tttttgctga agaattgtcc cgtcaggaat tgaacatgag
    25921 tgatcagtgg atcgatatac ctgaagaagt acgtgataaa tatatgatat atcgccctac
    25981 tccacttgta agagcataca atctggagaa agcgttggat acaccggctc atatttactt
    26041 taaaaatgaa agtgtgagtc ctgtgggttc acacaagttg aactctgcat tagctcaagc
    26101 ctattattgt aagaaacaag gcattacaaa tatcaccacc gaaacaggag ccggacaatg
    26161 gggaaccgcc ctttcatttg ctgcaaaaat atttggtctg gaactggctg tgtacatggt
    26221 caaaatcagc tacgaacaaa aaccgtatcg ccgttcactc atgcaggcat ggggtgcaca
    26281 agttattgca tcgcccagca tgtcgaccaa agctggccgg aaaataatta ctgaacatcc
    26341 gaattatcag ggaagtttgg gaactgccat ttctgaagca atagaactgg ctcttcaaac
    26401 gccaaactgc cgctatacac tgggtagtgt acttaaccat gtttcacttc atcaaaccgt
    26461 catcggactt gaagctgaaa aacaaatgga gatggcagga gaatatccgg atattattat
    26521 cggatgtttt ggaggaggtt ctaacttctc aggcatatcc tttccgttca tgcgtcacac
    26581 aataaatgag ggacgaaaaa cacgctatat tgctgcagaa ccagcatcat gcccaaaact
    26641 gacacgtgga gttttccagt acgattttgg tgacgaagct ggctacactc ctctccttcc
    26701 gatgtttaca ctgggacaca attttgcacc ggctcatatt catgccggag ggttgcgtta
    26761 ccacggtgcc ggaaccattg tcagccaact gatgctcgat aagtatatgg aagctgtgga
    26821 cataccgcag atcgaatcat ttgaagcggg tattttattt gcccaaactg aaggtattat
    26881 gcctgcgccg gagtcggcac atgccatagc ttcggcaatc cgcgaagcaa aacaggcaaa
    26941 ggaagaaggc gtgaaaaaaa caattttatt taatctctcc ggacatggat ttgttgatat
    27001 ggccgcttac gatcagttta ttgcaggcga tttaaccaac tatgaactca gtcaggaaag
    27061 cattgataaa actacaggat tgttggaaaa aataatctga gaataattta tcttataaaa
    27121 aagagcgtaa tccgattggt attacgctct tttgttaaat agaactttat ttaatgaaca
    27181 tttttttcat ccggatttat tatcagtcgc agggatgtgt cttttgttac gtagctccag
    27241 gcccagttaa tgaagataat cagcttgttt cttacgctca gaattaacat caggtgcaag
    27301 aacatccaca caaaccaggc aaataaaccc tgaaatttcc agaacttaag atctacaacc
    27361 gctttattct ttccgattgt agccattgag cctttatttc tatattcata ttcagtcgtt
    27421 ttctttcctt taagcatttt ttttatatta tcagctaaat tttttgcctg agtaattgcc
    27481 acattagcaa cctgaggatg acctttcgga taatcgggag tttccatata ggcaatatcg
    27541 cccactgcat atacattctt tgtgtctttc accaggttat ttctgtcaac aacatatcgg
    27601 tttcttacta aattatcagg agaaccaact ccctgtatga cattgccggt aacaccagca
    27661 gaccagatca gagtactgca acggattgtt tctccggtgt ttaactttgc agtattacca
    27721 tcataactat ttaagataac accggtctta acttctactc ccagatcttc cagatacttt
    27781 ctcgacattt tttttgatac atcactcatc acactaagtg taaaaggact tccttcgagc
    27841 agtataattc tgagttttga gaaatcgacg ttggggtaat cttttggtaa aatgtcattt
    27901 ttcaattcag caaaagaacc ggcaagttct acacccgtag cgccggctcc tacaataatt
    27961 atattcatca gggcttcctt tgcttcaaag ggggcggttt gataagcctc gaaattgagc
    28021 aaaatccggt tgcgaatttc aatcgcttcc tgcatggttt tcatggcaag tgagtttttc
    28081 tttatttcct cattgccaaa ataattagtc gtacaacctg taccaataat caaatgatcg
    28141 taatgcacaa ctccattgtt agtaatcagc atattattgc ccgtatcgac acttttcact
    28201 tctgccatcc ggaattcaaa gtttctgtaa ttctgaaaaa cctttcggaa tgggaacgaa
    28261 atgcttgccg gttccagacg ggcactcgca acttgataaa acaatggctg aaacgagtga
    28321 tagttctgtt tatcaatcag tgtaatacgc agtggtgtgg ttttgagttc ctttaccagt
    28381 cgcatgccag caaaccctcc tccaacaata accacatgat cttttggtct aaaattcatc
    28441 atcttttact ttttaatttg agaatgcaaa attacaacaa ctacttcaat aagaaaacaa
    28501 attaaagaaa tatttcttta attcacgcag ttaattcgga acaaaaattt tcaattttac
    28561 aaatctcaat aaataacaga tattcaattt tatataaggt tatttcatgc tctttatgga
    28621 atactgaaat caattttctt gatttaatgc aaaaataatt ttttcattct aaaaataaag
    28681 tgtatctttg cactcgcttt tgagaaagta tttcggggtg tagcgcagtc cggttagcgc
    28741 acctgctttg ggagcagggg gtc
//
LOCUS       DAOPYB010000021        26834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1766_length_26834_cov_8.388028, whole genome shotgun sequence.
ACCESSION   DAOPYB010000021 DAOPYB010000000
VERSION     DAOPYB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26834
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1766_length_26834_cov_8.388028"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKH58_03105"
     CDS             complement(<1..626)
                     /locus_tag="PKH58_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88047.1"
                     /translation="MKIQVIAFLLGLCPFIAAQQPAIYQSKAFSIFPNKVIQGDFNAN
                     VISPIELTSNYRSPETDKYSPTVKFKFSINSRDNEMPSGIDHFVTLVPENGKCITNVV
                     FGTQLKQTQAIPTGINLPENTVWIIRLDMRKVFKAFETDGYYILYNGDKLAKDDFRGV
                     YVAGSAAPLSWDFSNLYNKSGLQLTDADGDSIYETTLIMNKKQDIKST"
     gene            complement(649..2430)
                     /locus_tag="PKH58_03110"
     CDS             complement(649..2430)
                     /locus_tag="PKH58_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 66 protein"
                     /protein_id="HNX88048.1"
                     /translation="MSTLNSNFKYLIILPLIACVSFSCKGAEPDVTPNAPANVATPTY
                     VNVSIATDKAVYKPGDNVIFTIDNSALPATAKVRYKNANTVISEANVTGSTWSWTTPT
                     TDFKGYIAEIYTTVNNVETIYATIGIDVSSDWKKFPRYGFLSKFGILSTDEINGVISN
                     LNRFHINGLQFYDWQNKHQKPLPMSGSVPASSWKDIGNRDIYFSTVEKYINAAHGYNM
                     KAMFYDLVYGAWDNAETDGVDKEWYIYTDNTHTNRDIFALSSPFLSNLNLLDPANSGW
                     QNYMANETKNVYKYLAFDGYHMDQLGDRGTRYKYDGSFMNLSGSFKSYIDAIKAAAPE
                     KYNVMNAVAQYGQQGIATSTSDFLYSEVWSPSDSYNDLVNIIKQNNTMSSNSKNTVLA
                     AYMNYDKANTVGYFNTPSILMTNAVIFAFGGAHLELGEHMLCKEYFPNDNLAMKDDLK
                     QSLVNYYDFLVAYQNLLRNGGTFNTITIASTDNKISLSAWPAAQGKVAVFGKKFDNSQ
                     VIHLINFTNSTTQQWRDKNGIQVMPVLVKDTKLLLTSDRTVKNIWIASPDVAGGASRK
                     LNFAQVGNKVSFILPELRYWDMVVIEY"
     gene            complement(2455..5511)
                     /locus_tag="PKH58_03115"
     CDS             complement(2455..5511)
                     /locus_tag="PKH58_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX88049.1"
                     /translation="MKIKLSILILFLLYIQVNAQTSFVAESGVAIFCPADMDSVRTLP
                     SLALVKNLQPQGSVPVNWKITPVFSKVNGKSTVTITYDVDADLYGNGEVTGSLRRNNT
                     NVTLWNTDNYLYTSVDNGKRLYQSHPWVMGVRKDGTAFGIIADNSWKQYFDLSNPLTI
                     TSEGPAFRVIIIEKQNPMEVQKTLAELTGKMNLPPLWAIGYQQSRYTYSPDSRVKKIA
                     DEFRNRKIPADVIWMDIDYMSNYKVFTFNPTTFSNPKGLNDYLHAKNFKAVYMIDPGV
                     KKETGYTVYDSGVAGGHFVRTSTGQEFNGTVWPGNCAFPDFTRPATQSWWAGLYSNFM
                     SLGIDGVWNDMNEPSVFNGTNGTMPEDNIHRGGDDLPAGSHARYHNVYGMLMVKSSRD
                     GLIAANPDKRPFILSRSNFLGGQRYAATWTGDNKSTWDYLKMSIPMCINLSLSGQPFN
                     GPDIGGFEGNCDAELLGHWMALGAYYPFSRNHSSNNTIDQEPWAFGTPIENVSRTALN
                     RRYRLMPYLYSLFREASISGTPIMQPVFFADYIDANLRSEQQLFLLGSDLLIAPRWAT
                     NVSFPKGNWNQIKFENTDDGYQPYVFLRPGAIVPITGIIQSTAEYKTDSVTLLINPNT
                     DGSARGKLYDDAGNGYDYQTGDYAVHQFVSSKYHADSLKVIIQRNEGNRRVNRKYRIG
                     YVLNNTITYSNWSKDTVQYVKIVTDSQPEIDKTLLPSMYISGVFDKKNQTILPMKYLG
                     NNKWVTDTIFLSVGTQSFKFLAQKDSSSGSWGNALGISGIAKAGLPTAPDITCTITEV
                     GKYIINFNQLTLRYSVSKAPIYNYLSIVGDATAIGWNPTGMPMIQDSTNLNLFSLKSL
                     LTPGTFKFHTTNGDWCNGDWLLANVQDQSLSSTNYKVFTGCPPTDADLKWKVTTAGNY
                     EIKVDVGISKITINQQNPQGVGNILVGGIKLYPNPTANELNIDLGEERSAELSIYTAS
                     GKVIYQSKLSKCLNIIDLNKLIKEGTYFIKIKTKTESIVSKLIFKV"
     gene            complement(5527..8034)
                     /locus_tag="PKH58_03120"
     CDS             complement(5527..8034)
                     /locus_tag="PKH58_03120"
                     /inference="COORDINATES: protein motif:HMM:NF037003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX88050.1"
                     /translation="MHRKIYYIITCLFCFSLVNVSSQNPSNPYKAPLYWDVYENNFLK
                     EKAGVTNNYISEDEFLANINWIDTNMKSSGYNMICIDGWGDADYNVNGYRTKHSSAWA
                     HDYAWWSAELQKRGMTLGIYNNPLWVNTNAANAGLKVKGTNISLSSLINTNENALWFT
                     WLQVDRPGAEEYVKGYIQYYADMGVKFLRVDFLSWFESGYDRNVGTVGPDRRNSYING
                     LSYYETALKWMREACDANGMFLSLVMPNLYTDANIEQKYGHMVRVDEDCAEGGWYRFS
                     DSSRGQQRVGWSQFANPFDGYVYWSKIAGRDKMILDGDFIRLNTYANDEERKCVVSMH
                     LMAGGPVSIADQYNTIGSNSWIYQNKEMLALNQDGFVGTPLSNDPTNELNQVWKGQLS
                     NGDWIVGFFNREGSAQSRSISFQNDLGIFGNAFVRDLWAHTDLGVVSLLNKSVPAHGC
                     MIFRIKSNVNKVISPLFSLKEGSYNTSCSVSLSTATSGASIYYTIDGSIPTSASSLYT
                     VPVTISKTSTIKAIAIKSGMEDSYVNKSTYMIGEAMPQASINIGATFNNWDLTATPMK
                     NTDGNNWTTDPISILTGDHIMKFANTTNWSGDDWGNASGLSGTTQLTTGGGPFITFKT
                     AVTGKYLISFNDYSLTYNVQKILDFVQSQMYVAGSFNNWSLTNGKMTLMDNNLWKSSA
                     ITIPAGTYQMKFANTSNWTGDDWGNTSGLTGVASLATGGLPNISFTVPTSGSYIISFN
                     DATLAYSVVNSVTEDPELKISDIHVSSSGKNIVIDKNNNDDVSIEVISLNGQLIYKTN
                     TTSTTTVINLSSQQSNSLIIVRVITKSKIQSTKLLLN"
     gene            complement(8116..9060)
                     /locus_tag="PKH58_03125"
     CDS             complement(8116..9060)
                     /locus_tag="PKH58_03125"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HNX88051.1"
                     /translation="MKTSLLSKLIAVTLFSTVAFISVNAQNPMCMIGDATKYGWDKDK
                     SSPMTQDGVNLSKFYYNAYLNAGSFKFLMQNSDWVPSWNKGADESTVVKRKTDSDPDN
                     SFSISTAGNYSVVLDTAALTLSVTPMAETTPIPFNTVFMVGNAAPNGWDLGKATELTK
                     NPTNPFEFSYTGALSVGEFKFPVNRNWGWNQDFFIKVSDTQMQLVAGGDVKWSITEAA
                     NYKVTINTNTLAISIEKQITDLNPAIENSQLSLKSNVVKDQLTVLSLNSADYLIFSSV
                     GTLVQRGAFTGSSINISNLRNGIYIVKIENKSFKFIKE"
     gene            complement(9107..9283)
                     /locus_tag="PKH58_03130"
     CDS             complement(9107..9283)
                     /locus_tag="PKH58_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88052.1"
                     /translation="MKTEINKKRIYLTPEVERIKLDNEISLQMESVPPTGPNEISGSN
                     TIIYHNNDPFRNMA"
     gene            complement(9404..11656)
                     /locus_tag="PKH58_03135"
     CDS             complement(9404..11656)
                     /locus_tag="PKH58_03135"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HNX88053.1"
                     /translation="MKTTLLSKLVAITLFCVITFMSVNAQNPMCMIGDATKYGWDKNK
                     SSPMTQDGVNLSKFYYNAYLNAGSFKFLMQNSDWVPSWNKGADESTVVKRKTDSDPDN
                     SFSISTAGNYSVVLDTAALTLSVTPMAETTPIPFNTVFMVGNAAPNGWDIGTATELIK
                     NPANPFEFTYTGALTTGELKFPVNRNWGWYQDFFMKVSENQMVLQNSPDSKWVISESG
                     NYSITLNTNTLAIDIRKLNTWLGSTGNWSAGSNWSTGSAPTNSEVVDINNAEVAIDQD
                     VTAYQINVHAGAKLTINQDKSLTVSKLKLLSGSTGTGTFVNNGTVTVTTAEVQQYLAS
                     SRNWYISSPISNGTVPSGLTYYGYDETGSNASYSAPATEYWVANSAGTTLVQGKGYIA
                     QPGIATTIGFSGTLNDGNIDIDLTRSGATYTGFNLVGNPYPSYLKFSDVSVSNSNIQT
                     TMWYRTKNISNTYMFATYNASGDILIAPNANTMVTGYIPPMQAFWVKATFASTLTFTN
                     AMRSHIDVSGNVIKVKAAQANPILRLQVSNGVNSDEAVVYFNPNAINTIDKYDSEKMS
                     NNNSSIPEIYTTPVVGTANLAINGMNSVNAVNELPLGFTTGETNNFTIKASEISNFDA
                     DTQIILKDNLLGTETDLTAGNSYSFASDATSTADRFTVVFKTSGVATDVNKTICNNNF
                     VFANGNLQIGVYSKKAIGNVSVFNAVGQKVADRALNATSSYINVPEAGMYMVSISNNG
                     VTNARKVMVK"
     gene            complement(11740..13155)
                     /locus_tag="PKH58_03140"
     CDS             complement(11740..13155)
                     /locus_tag="PKH58_03140"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HNX88054.1"
                     /translation="MKKIRIYNVVLLVSLLTVGLFSSCEQNLPDNIVKAGALALSASK
                     TTIELNQKNSSNEAISLTWTTGTNHGTGASISYVLQIDKKGNNFTNAITEDMGKGIYV
                     KSFTVGELNDSLLNRWVSTPGTETELEARVISTVYSTPSVNDTSAVITILVTPYQPVS
                     KTLYLYGSASPKGTDLNNALALTPQSDPTVFVYQGSLAVGTFKFITTKGQLLPSYNKG
                     DDSTKLALRTADSQPDGQFSITEAGVYKIVVSLLDLSISYSKIDYPAYSEIYMVGGAA
                     PNGWDIANATKLTQSTSNPFIFTYQGVMQVGEFKFPVNRNTDWNQDMFERVDDTHMYL
                     HAGGTAGDDKWTITKKGFYTITLNQLDNTISIYREKLYMVGSATPIGWTITNAIQMTE
                     DATDGCIFTYTGPMVAGEFKFPVNRNSDWGQDMYMRTDDSHMYRHIGGQADDNKWSIT
                     SAGTYIVTANLETLSISFIKQ"
     gene            complement(13190..14683)
                     /locus_tag="PKH58_03145"
     CDS             complement(13190..14683)
                     /locus_tag="PKH58_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX88055.1"
                     /translation="MKLIKYISIAVIIVFFSACSDFLDKVPEDSINTSNFFQTEDDAI
                     GAINGAYQPLQWPKLYNMRMWTTDIMAGNSIVGAGGGTDGIETQDESNFVTSTDNQGV
                     LDMWRGPWPGILRCNIILQKVPEMTISENLKNRIIGEAYFLRAHYYFILVQFFGDVPL
                     VLKPVEPGDDLRPFRTPKTEVYKQIESDLSTAVTLLPEREQYSGANIGRASKGSAVGM
                     LAKVELTLGNYQRVVNLCDSVKSLGYALNANYADNFNPATENSVESLFEVQYVGGAGM
                     DFWSNENQASWLSTFTGPRGSNMAAGGWGWNQPTQEFVNAYEVGDLRKDVTVFYEGCP
                     KFDGMDYKKSYSTTGYNLRKFLVPLSVSSTYDNSPLNFPVLRYADVLLMKAEALNELG
                     RTAEAQLLATDDNATLNKVRVRAGLLNVTGLSQDELRNKILHERRMELAFEGQRWFDL
                     IRVKGGQYGIDFLHSIGKSNMSSKYLLFPVPQKERDTNPNLTQNTGY"
     gene            complement(14704..>16842)
                     /locus_tag="PKH58_03150"
     CDS             complement(14704..>16842)
                     /locus_tag="PKH58_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX88056.1"
                     /translation="TDWLGAFFRTAPMQNYSLNYSGGSDKTNIYVSGNYFNQDGIVLN
                     TGFKRFTFQLNTDTKVTSFLKFGNSLTLNHDIKSNGDYSIRNAMLALPTQAIYHEDGS
                     YTGPVGQPIYSGDITNPIGAAKTVQNSTNGYNLMGSVYGELEILKNLKFKSTFGLQAN
                     FWDSRTWSPKRAWDTSVNLESYLGEQFNKNLTWLWDNTFTYDKTFGKHKLGIMAGTSA
                     QENKYDYMNGSIQSFASDVTQQLNNGILLPKVGGSTSSYSMLSYMGRVNYAFADKYLF
                     TATLRQDGSSRFGEKNKYGLFPSASVAWRISEEDFFKPVTFVNYLKIRAGYGATGNQS
                     IGNYSFASSLNTIKYNFNNNVVSAVVPTVMPNPYVQWEQQKQGNIGLDATMFNQRVEV
                     TLDAYLKQTDKMLVPMVVPVSTGYSDVYVPSINAGTMENKGIELTVNTRNLTGELKWN
                     TMLNVSFNANKVVSINDTIPMSSGSIGLNQNLALIQAGLPINEFYGFVTDGIFQNQQE
                     VDAHAIQVPGSDPYNRTSPGDIRFKDLNNDGVINDKDRTYLGSPNPTFTFAMNNTFSY
                     KGLDLSIFLQGVAGNKICNANRFWSEAMAVAQNQTNATLNRWTGEGTSNTMPRAVFND
                     PNKNTRPSDRYIEDGSYLRIKNVTLGYTLPTNLVKKAQISNARLYISAQNLFTITKYS
                     GFDPEVGTSGIDNSVYPVTRTISFGINLGI"
     assembly_gap    16843..16942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17397..18806)
                     /locus_tag="PKH58_03155"
     CDS             complement(17397..18806)
                     /locus_tag="PKH58_03155"
                     /inference="COORDINATES: protein motif:HMM:NF037003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88057.1"
                     /translation="MKKKVLFLIYSPFFFLAAGCSQKEEWKPNITENEVVDTPWVTPA
                     NPYKAPLYWSPYEYNIEDEQNTHIGYIPENVWSDNINWVEKNLLPYGYDMVCIDGWGN
                     MVYDPTTGYRKSHSSNWTHDYAWWSKELKSRGMRLGIYDNPLWVNPAAANAGIKVWGT
                     QFNLSDLINWDESTSGFRWLQVDKPGAREYVDGFFKYYSQMGVNYLRIDFLSWYETGY
                     DQWMGTVGAAHGKENYEKALRWMRESADKYKVFISLVMPNLYNDAEVENKYGHMIRVN
                     EDTGDGGWWKFSDNARGQHRQHWSQYATAFDGYIYWSRLSGRKKMILDGDFIRINTFA
                     NNDEKKSVISLHLVAGGPVTISDQFNTIGNDLWLYQNEEMLALNKDGFVGKPLSNDPT
                     NPQSQIWKGQMTNGDWIIALFNRENYSINRSIDFSTLGISSASVRDLWSHKDLGSMAS
                     YSISLSAHACIVLKLVKNN"
     gene            complement(18806..19939)
                     /locus_tag="PKH58_03160"
     CDS             complement(18806..19939)
                     /locus_tag="PKH58_03160"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HNX88058.1"
                     /translation="MKTIKKIILLLLLTICVSACEDTGIVSNWLQNPVFEKPMTVVAS
                     TDSIILKEGHEKDTAVIFTWNKGNDRGAGTKLTYLFRMDIAGKSFETATNVEEMEPGI
                     FSKSITVGELNTLLLEHWGIPGGSTVKIDAEIIARVDSSKQYQKPELASVSFYAKGFS
                     LGPLPLYLVGSAISGGWDYSKGVKLTEIIERKQYSYTGNFSVGTFKVIESISNEFPSL
                     NPLSDSLLYYRTSAENKDSLFSISKSGRYTFYMNRSTLKYALAYTPYEKIYMVGAATT
                     AGWDINAPVQLNWDVKNPEVFTFTGSLLAGEMKFPLSTGNWGCDFLMPIANGTIINGD
                     ATDITTMQFVAGGNPDKKWIIKSAGTYKITVNPAKLTVVFKKL"
     gene            complement(19958..21829)
                     /locus_tag="PKH58_03165"
     CDS             complement(19958..21829)
                     /locus_tag="PKH58_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX88059.1"
                     /translation="MKKSNIRIAFLLFTLMLLSSCADFLDKEADTEITLESVFQNKTK
                     TQQWLAGCYSMIPDPYWGYMRDLGWEILGDDMSPSERWRNYGWQVIPFALGEWTIDSD
                     WSPSYWSVLPQKIRECNIFMQHVQALPDQKLSQEEVALMVGEARFLRAYYYSLLLNTY
                     GPIPFKPDYITPVNYNLSDLMVGQTPYDDIVNWIDTELTEVAKILPATYSDGGKYGRA
                     TSIMCEAVRARMLLFAASPLVNGNPDYVAHTNKEGKELFNTTYDASKWTKAATASKKL
                     IDDAEAAGHALYTELNDDGSIDPFLSCENLFLKEYSKGNTEILFARPSCDYSTYDKHS
                     APYGSSGNGGYGVTQSLVDAFFMANGLPKDDPNSGYSETGFSTSDGYFTTGKTKWKEC
                     KNVGQVTLAGTYKMYCNREPRFYLAVNFNGQWYNKDSRTLNFYYNGKDNPGTHDAPQN
                     GYLSRKRTDPSRDPINNVFAWRPGILYRLGEVYLNYIEALNEIDPGNPDILLYLNKIR
                     NRAGVREYSTGVTSTTVIHADLGDQTAMRELIRAERRVELCTEGIRYDDLRRWKQAES
                     VLNQNFYGMNFYGTNQTNFFTRTQYQTRVYKKAFYWFPVHLSEIEKNPNLVQAPFWE"
     gene            complement(21848..24931)
                     /locus_tag="PKH58_03170"
     CDS             complement(21848..24931)
                     /locus_tag="PKH58_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX88060.1"
                     /translation="MVNLKKSLFSVLFFVSMLFVLPNVVAQTTTKAISGIVVDTKGEP
                     LIGVSVLLKGTNTGTITNVDGKFTLQLGAVTAPTLVVSYIGYVKQEITPNVFSNFKIT
                     LEESSKDLSEVVVVGYGTQKRASVVGAITSVTPALLQVGTTPNISNNLAGQLAGVIGV
                     QRSGEPGYDASDIWIRGISTFAGSTSPLVLVDGIERSLNDLDAAEIESFSILKDAAAS
                     AVYGVRGANGVILVNTKRGFVGKPKIEIRTEQSVQQPTQLPSFIGAAEYMTLLNELAN
                     NAGYTKPFSNDRIVKTFNKYDPDLYPNINWIDAITKSYASNTRTNLNVAGGSDILRYS
                     LTASYYGENGIIARDPAQTYSSESRMHRYNIRSNVDINLSKTTLLRVNIGGYLQELQK
                     GLNSTDDLFSAAFETPPYVHPTVYSDGTFPIVAQRTNPWAYSTQTGYYRKGESKIESL
                     FSLEQDLKMITPGLKAKFTFSFDSYSANYVNRGKSPNYYNVATGRDVEGNLIHSIYSY
                     GQEYLGLSTNADWGNRGTYLEGNLYYNRTFGKHDIDAMLLYNQNSYDNGGIQPYRHQG
                     VAGRLSYIYDTRYVGEFNFGYNGSENFAKGQRFGLFPSVALGWLLSEEKFMQPYKKIF
                     NKIKFRGSYGFVGNDNIGSNRRFAYLTTLNTNVVGYTWGTGNGSYYSGIQEGEIGVNN
                     LTWEKVAKANVGIELGILGMVDLQVDAFNEDRKDIFIQRTTVPTQAGFLSTPYANYGK
                     MNNKGFEVALSMNKQITKTLFMNWRANFTYAKNTVLEKDEAAAVLGTYRSATGLPNNT
                     LWGYTADRLYTSADFDGSGNLVSGQAIPQIGGTPVRPGDIKYIDKNKDGIINSLDEGY
                     IGGTLDPQIVYGFGNTTKYKQFDLSFFFQGVANTYQIIGQGSGSSFFIPGSGQGVLGN
                     IYSNYNDRWTEENPSQNVFWPRLDYGTNYNNSAPSTWWKKDRSFLRLKTLELGYSLKK
                     SLIEKAKLSGVRIYLSGNNLFYLSSFNLWDPELATSTGLKYPSMKSVLFGIQVSL"
     gene            complement(25218..>26834)
                     /locus_tag="PKH58_03175"
     CDS             complement(25218..>26834)
                     /locus_tag="PKH58_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6377 domain-containing protein"
                     /protein_id="HNX88061.1"
                     /translation="YFVLSNSLFADNGIDSLLKVLDRTVDNYQVYSNKKEEKMNKMKD
                     LLRYTTSYDQKYEICGKLYDEYSSYKSDSALVYARKKLQIAKKMNNKRNLADARLNLA
                     SIMGVSGMYKESMDIMSKVNINEAPDLKSYYFHIYRTVYGFMVDYTVSSQEKKRYEVI
                     AESYRDSLLQTIIPNSGPYIMVKSDELISQKRYDEASTLLFNYYPTIKDNLHDKAIIA
                     YCISLTYNRKKNKTEEKYWLAVSAIHDIESATKDYISLRRLAFILNEEGDVTRAYKYM
                     KRSLDDALFCNARLRTFEVSKMMPIIDKAYQQQTESRQRLMLISLSSISVLSFLLIVA
                     IFLVYRQMKKLAVARKNLSVANEQLISLNQELSSTNNQLKHTNETLQESNLIKEVYIG
                     RYIDQCSVYLNKLDEYRRHLNKISIAGKKDELLNEIKSTKIIDEELKEFYSNFDSTFM
                     QLFPSFIEELRELMIDDEEIKLKSGEILNTGLRVFALIRLGIKDSDKISFFLRYSATT
                     IYNCRTRFRNKSKGPRNEFEAKVMQIGVFK"
BASE COUNT         8037 a         5315 c         4563 g         8819 t
ORIGIN      
        1 tcagtagact ttatgtcctg ttttttattc attatcaggg ttgtttcata aatactatcg
       61 ccgtcagcgt cggtaagttg taagcccgat ttgttgtaaa ggttgctaaa atcccatgac
      121 aacggtgcag cactacccgc aacataaaca cccctgaaat catctttagc aagcttatcg
      181 ccattataaa gtatgtaata accatcagtt tcaaatgctt tgaatacttt tcgcatgtca
      241 agtcgaatta tccaaactgt attttcgggt aaattgattc cggtaggaat agcctgagtt
      301 tgttttaact gagttccaaa aaccacattc gtgatacatt tcccattttc gggaactaaa
      361 gttacaaaat gatcaattcc tgaaggcatt tcattgtcgc gagagttgat gctaaactta
      421 aattttacag ttggactata tttatctgtt tccgggctac gataattgga cgtaagctca
      481 ataggtgaaa taacattggc attaaaatca ccctgaataa ctttattcgg gaagatgcta
      541 aatgctttcg attgatatat tgcaggctgc tgagccgcta taaatgggca aagacctaat
      601 aaaaatgcga ttacttgtat tttcatattc attctaacaa ttatgaaatt aatattcaat
      661 tacaaccata tcccaatatc tcaactccgg aagtataaaa cttaccttgt ttccaacttg
      721 tgcaaagttc aattttctgg atgcaccgcc tgcaacatca ggcgaagcaa tccaaatgtt
      781 tttcactgtt ctatcgctcg ttagaagtaa ttttgtatct ttcaccagaa caggcattac
      841 ttgtatgcca ttcttgtcac gccattgctg tgtagttgag ttggtaaagt tgatcaagtg
      901 aatcacttgt gaattatcga atttctttcc gaatacagct accttacctt gtgcagcagg
      961 ccatgccgaa agggatattt tattgtcagt tgaagcaatt gtaatggtat taaatgttcc
     1021 tccgtttcgc aacagattct gataagctac cagaaaatca tagtagttga ctaatgattg
     1081 cttcaagtca tccttcattg ccagattgtc gttagggaaa tattccttgc aaagcatatg
     1141 ctcgcctaac tcaagatgag caccaccgaa agcaaatatc acggcattgg tcattaggat
     1201 tgaaggtgtg ttgaaatagc caacagtgtt ggccttgtca taattcatgt aagccgctag
     1261 aaccgtattt ttagaattac tgctcatcgt attgttttgt ttaatgatat tgaccaaatc
     1321 attataacta tccgaaggac tccaaacttc ggaatataaa aaatcgctgg ttgatgtggc
     1381 aattccttgt tgaccatact gcgctaccgc attcattaca ttatattttt cgggtgcagc
     1441 tgcttttata gcgtcaatat atgatttaaa agaccctgac agattcataa acgagccatc
     1501 atatttataa cgagtgcctc tatctcccaa ctggtccatg tgataaccgt cgaaagccaa
     1561 gtatttatac acgtttttgg tttcattagc catataattt tgccagccac tgtttgcagg
     1621 gtcaagcaga ttcagattgc ttaaaaatgg agatgaaaga gcaaatatat cacggtttgt
     1681 atgtgtatta tcagtgtaaa tataccattc tttgtcgaca ccatcagttt cagcattatc
     1741 ccatgctcca taaaccaaat cataaaacat ggctttcata ttgtaaccat gagcggcatt
     1801 aatatacttc tcgacggtac tgaaataaat atccctgttc cctatatctt tccaacttga
     1861 tgcgggaaca gaaccactca ttggcagtgg tttttgatgt ttattttgcc aatcgtaaaa
     1921 ctgcagacca ttgatatgga aacgattcag attactgata accccattaa tctcatcggt
     1981 agaaagaata ccaaacttag acaaaaagcc ataacgtgga aattttttcc aatcacttga
     2041 tacgtcgatt ccaattgtag cataaatggt ttctacatta ttgaccgtgg tataaatttc
     2101 agcaatataa cctttaaaat cggtagttgg agttgtccac gaccaagtag agcccgttac
     2161 attagcctct gaaatgacag tattagcatt tttatatctt accttagctg ttgcgggaag
     2221 cgcggaattg tcaatcgtaa aaatgacatt atctccgggt ttatataccg ctttatcggt
     2281 agctatactc acattgacgt aagttggtgt agccacattg gctggtgcat ttggcgtaac
     2341 atctggttcg gcacctttac aactgaatga aacacatgca atcaaaggta aaataatcag
     2401 gtatttgaaa ttggaattaa gagtgctcat ggtatgtttt ttttgtttat aattctatac
     2461 tttgaaaatg agtttactaa ctatagattc ggtttttgtt tttattttaa taaaataagt
     2521 tccttctttt attagtttgt tcaaatcaat aatatttaaa cacttggaca atttggactg
     2581 ataaataact ttcccagatg cagtgtaaat agataattca gccgatcttt cctcccctaa
     2641 atcgatattc aactcatttg cagtagggtt gggatataac tttatgccac ctaccaatat
     2701 gttacctacc ccctgaggat tttgctgatt tatagtgatt ttggaaattc ctacatcaac
     2761 tttaatttca taattaccag cagtagtaac tttccatttt aaatcagcat ctgtaggggg
     2821 gcaacctgta aaaaccttat aatttgttga ggaaagcgat tgatcttgaa cgtttgccaa
     2881 taaccaatca ccattacacc aatcgccatt agtagtgtga aacttgaaag tacccggagt
     2941 caataaactt ttcaagctaa acagattcag attagttgag tcctgaatca ttggcatccc
     3001 agtaggattc caaccaatgg ctgtggcatc cccaactatt gaaaggtagt tgtaaattgg
     3061 agctttcgat actgaatatc tcaatgtcaa ctgattgaaa ttgataatgt atttaccaac
     3121 ttcggtgatt gtacatgtaa tatctggggc agtaggtaat cctgctttag ctattccact
     3181 tattcccaat gcgttacccc agctgccact cgaactgtct ttttgagcca aaaatttaaa
     3241 tgattgtgtt ccgacagaca agaaaatagt atcagtaacc cacttattat ttccgagata
     3301 cttcatgggt aaaatcgttt gatttttttt atcaaacact ccactgatat acattgaggg
     3361 taacaatgtc ttatcaattt ccggttgaga gtcggtaaca atttttacat attgaactgt
     3421 atcttttgac cagttagaat aagttattgt attattcaaa acatatccaa ttcgatattt
     3481 gcgatttact cttctattgc cttcgtttct ctgaataata actttcaatg aatctgcatg
     3541 atatttagaa gaaacaaact gatgtacagc ataatctcca gtctgataat cgtatccatt
     3601 accagcatcg tcgtatagct ttccccgggc tgaaccatct gtattaggat ttatcagtaa
     3661 agtaactgaa tctgttttat attcagcagt actttggata atacccgtta taggtacaat
     3721 ggctccagga cgaagaaaaa cataaggttg atagccatcg tctgtatttt caaacttaat
     3781 ctgattccaa tttccttttg gaaaacttac attggttgcc caacgtggag ctatcaataa
     3841 atcacttcca agcaaaaata gctgctgttc agaacgtaga tttgcatcta tatagtcagc
     3901 aaagaaaacc ggttgcatta ttggagttcc acttatagaa gcctccctaa ataaactgta
     3961 aagataaggc ataagtctgt aacgacgatt taatgctgta cgcgaaacat tttcaatagg
     4021 cgtcccaaat gcccatggtt cctggtcgat tgtattattt gaactatggt tccgcgaaaa
     4081 agggtagtaa gctcccagcg ccatccaatg tcctaataat tcggcatcgc aatttccttc
     4141 aaagccacca atatcaggac cattaaatgg ttgtccggat aaactcaagt tgatacacat
     4201 tggaatagac atcttaagat aatcccaggt agatttattg tcaccagtcc aggtagctgc
     4261 atagcgttgt ccacccaaaa aattagaacg agataaaatg aacggacgtt tgtcaggatt
     4321 agcggctatt agaccatccc ggctcgattt aaccatcaac ataccataaa cattatgata
     4381 tctggcatgt gaaccagcgg gcaaatcatc tccgcctcta tgtatattat cctcaggcat
     4441 agttccgttt gtaccattaa aaacagaagg ttcgttcata tcgttccaaa caccatctat
     4501 tcccaaagac atgaaattac tgtaaagtcc tgcccaccag ctttgagtgg cagggcgggt
     4561 aaagtcgggg aaagcacaat tacccggcca tacagtgccg ttgaattctt gtcctgtgga
     4621 agttctcaca aaatgcccac ctgctacccc tgaatcgtat accgtatatc ctgtttcctt
     4681 tttcactccg gggtcaatca tatacactgc tttaaaattc ttggcgtgta aataatcatt
     4741 tagccctttg ggattagaga atgttgttgg gttgaatgta aaaaccttat aattggacat
     4801 ataatcaata tccatccata tcacatcagc cggaatttta cgatttctga attcgtcagc
     4861 tatttttttt acacgtgaat caggggaata agtgtatctg ctttgctgat aaccaatggc
     4921 ccaaagtggt ggaagattca ttttaccggt aagttcggca agcgttttct gaacttccat
     4981 tggattttgt ttttcaataa ttattacacg aaaagccgga ccttcggaag taatcgtcag
     5041 tggatttgac aagtcaaagt actgcttcca gctattgtct gcaataatac caaaagcagt
     5101 tccgtctttg cgtacaccca ttacccaagg atgcgattga tagagtcttt ttccgttatc
     5161 aacagaagta tacaaatagt tatctgtatt ccataaggtt acatttgtat tattacgtct
     5221 taatgaacct gtaacttctc catttccata tagatcagca tctacatcat aagtaattgt
     5281 aactgttgac ttaccattca cttttgagaa aaccggagtt attttccagt ttacaggtac
     5341 tgaaccctga ggttgcaaat tcttcaccaa tgctaacgag ggtaatgtac gtacagaatc
     5401 catgtctgca gggcaaaata tagcaactcc ggattcggca acaaacgaag tttgcgcatt
     5461 aacttgaata tataacaaaa ataaaattaa tatgctgagt ttgattttca ttttaattca
     5521 tcagagttaa ttcaacaata attttgtaga ctgtatttta gatttcgtta taacccgaac
     5581 tataatgagg ctgttcgatt gttgtgaact tagatttatt acagtagttg ttgacgtagt
     5641 attagtttta tatataagtt ggccattaag gctaattact tcaattgata catcatcgtt
     5701 attatttttg tcaataacaa tgtttttgcc cgaagacgaa acatgtatat cggatatttt
     5761 caattccgga tcttctgtaa ccgaattaac tactgagtaa gctaaagtag catcattaaa
     5821 ggaaataata taactcccag aagttggaac tgtaaaactg atattgggca gacctcctgt
     5881 agcaagtgaa gcaacaccag tcaaaccaga agtatttccc caatcgtctc ctgtccagtt
     5941 tgaggtgttt gcaaacttca tctgatatgt tcctgctgga atagtaatag ccgatgattt
     6001 ccataaatta ttatccatta aagtcatttt gccatttgtc aaactccaat tattgaaaga
     6061 tcctgcgaca tacatctgag actgtacaaa atcgagtatc ttttgtacat tataggttaa
     6121 tgaataatca ttgaaagaaa taagatattt tcccgtaaca gccgttttaa aagtaataaa
     6181 ggggccacct ccagtggtta attgggttgt tccgctgagg ccggatgcat ttccccagtc
     6241 atcaccagac cagttagttg tattcgcaaa cttcattata tggtctccgg tcagaattga
     6301 aattggatct gttgtccaat tatttccatc agtattcttc ataggagtag ctgtcaaatc
     6361 ccaattatta aaagtagccc cgatattgat tgacgcctga ggcattgctt ctcctatcat
     6421 gtatgtagat ttattcacgt atgaatcttc cataccactc tttattgcta tagcttttat
     6481 agttgacgtt tttgatattg tgacaggaac tgtatataaa gatgatgcag atgttggaat
     6541 actaccatca atggtataat agatagatgc tccggaagtt gccgttgaca aactaactga
     6601 acatgaagta ttgtaactcc cttcttttag actaaacaaa ggcgaaatta ctttattgac
     6661 attcgattta attctgaaaa tcatgcaccc atgggcagga acacttttat ttagtaaact
     6721 cacaacaccc aaatcggtat gcgcccataa atcccgaaca aatgcattac cgaaaattcc
     6781 caagtcgttt tggaaactga ttgatctcga ttgagcgcta ccttctcgat tgaaaaaacc
     6841 gactatccaa tcaccatttg acagttgtcc tttccatacc tgatttaatt cgtttgtagg
     6901 atcattactt aaaggtgttc caacaaaacc atcctgattt aacgcgagca tttctttatt
     6961 ttgataaatc caggaatttg agccaatggt attgtattga tctgcaattg aaaccgggcc
     7021 tccagccata agatgcatgg aaacaacaca tttacgctct tcatcgttag catacgtatt
     7081 caatcggata aaatccccat ccaggatcat tttgtctcta cctgctattt ttgaccaata
     7141 tacatatccg tcaaaaggat tggcaaactg agaccaacca actcgttgct gtccacgtga
     7201 gctatcgctg aaacgatacc acccgccttc ggcacaatct tcatcaacac gtaccatgtg
     7261 accatatttt tgctcaatat tcgcatctgt atacagatta ggcattacta aactcaaaaa
     7321 cataccattt gcatcacacg cctcacgcat ccattttaaa gctgtttcgt aatagcttaa
     7381 accattgatg taactatttc gtctgtcagg gcctactgtc cctacattcc tgtcatagcc
     7441 actttcgaac cacgagagaa aatctacacg taaaaattta acccccatat cggcatagta
     7501 ctgaatgtat ccttttacat attcttcagc tccaggtcga tcgacctgaa gccaggtaaa
     7561 ccacaatgca ttttcattgg tattaatcaa acttgaaagt gaaatgttag tcccttttac
     7621 tttcaaccca gcattagctg catttgtatt tacccaaagc ggattattgt aaataccgag
     7681 tgtcattccc cttttctgaa gttcagcgga ccaccatgca taatcatgcg cccatgcaga
     7741 agaatgtttt gttctatatc cattcacatt atagtccgca tcgccccaac catcaataca
     7801 aatcatattg tatccagagc tcttcatatt tgtatctatc cagttgatat ttgcaaggaa
     7861 ctcatcttct gaaatataat tatttgttac accggctttc tccttcagaa aattattctc
     7921 ataaacatcc cagtacaatg gagctttgta aggatttgaa ggattttggg atgaaacgtt
     7981 gactaatgag aaacaaaaaa gacaagtgat tatataatat atttttctat gcatgtattt
     8041 gattttagga gagaacttaa ctttctatta atcaactccc attcgagttt tcgaatggga
     8101 gtattttaga gtctgttatt ctttgataaa tttgaatgat ttattttcaa ttttcactat
     8161 gtatattccg tttctaagat tgcttatatt gattgaactt ccagtaaaag ctcctctttg
     8221 aaccaaagta ccaactgagc taaaaatcag ataatctgca ctatttaaac ttagcactgt
     8281 caattggtct tttacaacat ttgatttaag agataactga ctattttcaa tagcaggatt
     8341 taaatctgtt atttgttttt caatgctaat ggccaatgtg tttgtattaa ttgtaacttt
     8401 ataattcgca gcttctgtta tactccattt tacatcacca ccagccacta gctgcatttg
     8461 agtatcagaa actttgataa agaaatcctg attccaaccc cagttgcggt ttaccggaaa
     8521 tttgaattct cctactgata atgctccggt ataactaaat tcaaatggat ttgtcgggtt
     8581 cttagtcaac tctgtagctt tacctaaatc ccaaccattt ggggctgcat ttccaaccat
     8641 aaatacagta ttaaatggta ttggtgttgt ttcggccatt ggtgttacac taagcgttaa
     8701 tgcagctgta tccaatacca cagaatagtt tccggcagtt gaaattgaga atgaattatc
     8761 aggatcactg tcggtttttc gttttacaac tgtgctttca tctgccccct tgttccaact
     8821 gggtacccaa tctgaatttt gcatcaaaaa tttaaaactc ccagcattaa gatatgcatt
     8881 gtagtagaac tttgatagat taacaccgtc ctgagtcatg ggagaagact tgtctttatc
     8941 ccatccgtat tttgtagcat cgccaatcat acacatagga ttctgggcat tcaccgaaat
     9001 aaatgcaact gtgctgaaca atgtaacagc aattaatttt gaaagtaatg atgttttcat
     9061 aactgattaa tttaaataaa ttgataattt tatattagta agaatattaa gccatgtttc
     9121 taaatggatc gttattatga taaataattg tattgcttcc actgatttca ttagggcctg
     9181 ttgggggcac agattccatt tgcaaggata tttcattatc caatttaatg cgctcaactt
     9241 caggagttaa atatattctt ttcttattta tttcagtttt cataattttt tcattttaat
     9301 ttatttgaga taaatctaaa atctcacgca aagacgcaaa agcgtaaaaa aatttgattt
     9361 taccgatatt tcggcatgtg ctatttgcgt gagagatttt ttattatttt accattactt
     9421 tccgggcgtt ggttactccg ttgttgctga tgcttaccat atacattcct gcttcaggaa
     9481 cgttgatata gctgctggtt gcgttcaacg ctctgtcagc tactttctga cctactgcat
     9541 tgaatactga aacatttccg attgctttct tgctgtagac accaatctga aggttaccgt
     9601 ttgcaaatac aaagttgttg ttgcaaatag ttttgttcac gtctgttgcc acacctgaag
     9661 ttttgaacac tactgtgaaa cgatcagctg tgcttgtagc atccgaagca aagctatagc
     9721 tgttacctgc agtcaggtct gtttctgttc caagtaaatt atctttcagg ataatttgtg
     9781 tgtccgcatc aaagttgctg atttcagaag ctttgatggt gaagttattg gtttcaccgg
     9841 tggtaaatcc taacggaagt tcatttactg cgtttacact attcatccca ttgatggcaa
     9901 gatttgcagt tcctactacc ggagttgtat atatttcagg aatagatgaa ttgttgttgc
     9961 tcatcttttc tgaatcatac ttgtcaattg tattaatagc attgggattg aaataaacaa
    10021 ctgcttcatc gctattcaca ccgttagaaa cctgaaggcg taaaattggg ttagcttgag
    10081 ctgcttttac ttttatcaca ttgcctgaaa catcaatatg ggacctcata gcattcgtaa
    10141 atgtaagtgt agaagcgaaa gtagctttta cccagaatgc ctgcatagga ggaatataac
    10201 ctgttaccat tgtatttgca ttcggagcta ttagtatatc tccggaagca ttataagttg
    10261 caaacatata tgtattgcta atattctttg ttctatacca catggtggtt tggatattgg
    10321 aattagaaac tgaaacatcc gaaaacttca gataagaagg ataaggattt cctaccagat
    10381 taaatccggt gtaagttgca cctgaacggg taaggtcaat atcaatatta ccatcattaa
    10441 gcgtaccgct aaaaccaatg gtagtagcga taccaggttg tgcaatataa ccttttcctt
    10501 gtactaatgt ggtgccggcc gaattagcca cccagtattc tgtagcaggc gcagaataac
    10561 ttgcattact accagtctcg tcatatccat agtacgttaa acctgatggt actgtgccat
    10621 ttgatatagg agatgaaata taccagttac gtgaggatgc gagatattgc tgaacctcgg
    10681 ctgtggtaac cgtgactgtt ccattattta cgaatgttcc tgttcctgtg cttccactta
    10741 ataacttaag ttttgaaaca gtcaatgatt tatcctgatt gatggttagt ttagctcctg
    10801 catgaacatt aatctgatat gcggttacat cctgatcaat ggcaacttct gcattgttaa
    10861 tatccaccac ttccgaatta gttggagctg atccggtgct ccagttagag cctgcactcc
    10921 aattcccagt acttccaagc caggtattta atttacggat atcaatggca agtgtatttg
    10981 tatttaatgt aatggagtaa tttccggatt cgctaattac ccattttgaa tcaggagaat
    11041 tttgcaatac catttgattt tcagagactt tcataaaaaa atcctgatac cacccccaat
    11101 tacggtttac cggaaatttc aattcaccgg ttgtcaatgc tcctgtatag gtaaattcga
    11161 atggatttgc cggattctta attaactcgg tcgctgtgcc gatatcccaa ccatttgggg
    11221 ctgcatttcc aaccataaat acagtattaa atggtattgg tgttgtttcg gccattggtg
    11281 ttacactaag cgttaatgca gctgtatcca ataccacaga atagtttccg gcagttgaaa
    11341 ttgagaatga attatcagga tcactgtcgg tttttcgttt tacaactgtg ctttcatctg
    11401 cccccttgtt ccaactgggt acccaatctg aattttgcat caaaaattta aaactcccag
    11461 cattaagata tgcattgtag tagaactttg atagattaac accgtcctga gtcatgggag
    11521 aagacttgtt tttatcccag ccatatttag tggcatcccc aatcatacac attggatttt
    11581 gagcattcac tgacataaat gtaattacgc agaacaatgt tatagcaact aattttgaaa
    11641 gtaatgttgt tttcataaac atgtaatttt atttataatt agaaagaggg attattctga
    11701 gttgtcagaa caacccctct ggaaaaacta aacaaccaat tattgcttta taaaacttat
    11761 ggacagtgtt tcaagattag cagtaactat ataggtgcct gcagaagtta tgctccactt
    11821 attatcatca gcttgcccac caatatgtcg atacatatgt gagtcatctg tacgcatata
    11881 catatcctgc ccccaatcgc tattgcgatt tacagggaat ttgaattctc cagctaccat
    11941 tggaccagta taagtaaata tgcagccatc tgtagcatct tcagtcattt gtatagcatt
    12001 tgtaatagtc cagccaatcg gggttgcact acccaccata tataattttt ccctgtaaat
    12061 acttatggta ttgtccaact ggttcagtgt aatggtgtaa aaaccttttt ttgtaatagt
    12121 ccatttatca tcaccagcag ttccacccgc gtgtaaatac atgtgtgtgt catcaactct
    12181 ttcaaacata tcctggttcc agtctgtatt tcggttaaca gggaacttga attcgccaac
    12241 ctgcatcacc ccctgataag taaaaataaa aggatttgaa gtactttgag tcaatttagt
    12301 tgcatttgca atatcccaac cattaggcgc agcacctcct accatataaa tttcgctata
    12361 tgccggataa tctattttgc tataggaaat agacagatcg agtaaactca caacaatttt
    12421 atatactccg gcttctgtaa tgctgaattg accatcaggc tggctatcag cagttcttaa
    12481 tgctaatttg gtactgtcat cccctttatt gtacgaaggc agcaattgtc cctttgttgt
    12541 aataaattta aaagtaccta cagccaatga tccttgatat acaaatacag tggggtcgct
    12601 ttgaggtgtg agtgccaagg cattattcaa atctgtacct tttggcgaag cacttccata
    12661 caaataaagt gttttactta ccggttgata tggagttacc aaaattgtta taacggctga
    12721 agtatcattc actgatggtg tggaataaac ggttgaaatt actcttgctt ccagttctgt
    12781 ttccgttcct ggtgtactta cccaacgatt taataaacta tcatttaatt caccaacagt
    12841 aaaacttttt acataaatac ccttgcccat atcttcagtt attgcatttg taaagttgtt
    12901 cccttttttg tcaatttgaa gaacatagga tatcgaagct cctgttccgt gattagttcc
    12961 agtagtccag gttagcgaaa tggcttcatt ggaactattc ttctgattca gttcaattgt
    13021 ggtttttgaa gcagataggg ctaacgcacc tgctttaact atattatccg gtaagttttg
    13081 ttcgcacgaa gagaacaacc caactgtcaa taggcttacc agcaaaacaa cattatatat
    13141 acgtattttt ttcatttcct ttaattatta tttaatggtt aaatattgct taataacctg
    13201 tattttgggt aagattagga ttcgtatcac gctctttttg aggaacaggg aataagaggt
    13261 atttagaact catattcgat tttccgatag agtgcaaaaa atctattcca tattgaccac
    13321 cttttacacg tatcaaatca aaccagcgtt gtccctcaaa ggccaactcc atgcggcgtt
    13381 catgtaaaat tttattccgc agttcgtcct gacttaaccc ggtgacattc aataatcccg
    13441 cacgaacccg aaccttattc aatgtagcat tatcgtcggt agccagcaat tgagcttctg
    13501 ctgttcttcc caattcattt aatgcttctg ctttcataag taatacatcg gcataacgaa
    13561 gaacaggaaa attcaatggg ctgttatcgt aggttgatga aacagaaagt ggaacaagaa
    13621 acttacgcag attataacct gttgttgaat acgatttttt ataatccatt ccatcaaatt
    13681 tcgggcaacc ttcgtagaat actgttacat ctttacgcaa atcacctacc tcataagcat
    13741 ttacaaattc ctgagtaggc tgattccatc cccaaccacc tgcggccata tttgagccac
    13801 gcggacctgt aaaagtgctt aaccatgaag cctgattttc gttgctccag aaatccatac
    13861 cggctcctcc aacatattgc acttcgaaaa gagattcaac agagttttct gtagctggat
    13921 tgaagttgtc tgcgtaattt gcatttaatg catatcctaa actttttact gaatcacata
    13981 gattaaccac cctttgataa ttaccaagag tcagttcaac ctttgcgagc atgcccacag
    14041 cagaaccttt agatgcacgc ccaatatttg ctcctgaata ttgttcacgt tcaggtaaga
    14101 gggtcacagc agtagatagg tctgattcaa tttgtttata cacttccgtt ttcggagttc
    14161 ggaacggacg caagtcatct cccggctcca ctggctttag cactaatggt acatcgccaa
    14221 aaaactgtac taaaatgaaa taataatgag cgcgtaaaaa atatgcttca cctataatcc
    14281 ggtttttcag attttcgctg attgtcattt ccggcacttt ctgcaaaata atattacaac
    14341 gcaagattcc tggccaagga cctcgccaca tatccaatac gccctgatta tcggtagagg
    14401 ttacaaaatt ggattcatcc tgagtttcta taccgtcagt tccaccgcct gcaccaacaa
    14461 tactgttgcc tgccataata tcggttgtcc acatgcgcat attgtatagt tttggccatt
    14521 gtaatggttg gtaagctcca ttgattgccc caatggcatc atcttcagtt tggaaaaaat
    14581 ttgatgtatt aatactatct tccggcactt tatccagaaa atcgctacag gccgaaaaaa
    14641 acactattat aacagcaata gaaatatatt ttatgagttt catatttatt tagatttaaa
    14701 tagttagata cctaagttaa taccaaaact gatagttctt gtaacagggt atacactgtt
    14761 atcaattcca cttgttccta cttcaggatc aaatccactg tatttggtaa tggtaaagag
    14821 attttgagcc gatatatata atctggcatt cgagatttgt gcctttttta ccaaattggt
    14881 tggcagtgta taacccagtg ttacattctt tattcttaaa taagaaccat cttcaatata
    14941 gcggtcagat ggtctggtat tcttgttcgg atcattgaac actgctcttg gcattgtatt
    15001 gcttgttcct tctccagtcc atctgtttaa cgttgcatta gtctggtttt gagcaacagc
    15061 cattgcttca ctccaaaaac gattggcatt acagatttta tttccggcta caccctgaag
    15121 aaaaatactt aaatcaagac ctttatagga gaatgtattg ttcatagcga aagtaaaggt
    15181 tggatttggg ctacctaaat atgtacgatc tttatcattt ataaccccgt cgttattcaa
    15241 atctttaaat cgaatatctc cgggcgaagt acgattatat gggtcgctac caggtacctg
    15301 aattgcatgt gcatcgactt cctgctgatt ttggaaaatt ccatcggtta cgaaaccata
    15361 gaattcatta ataggtaatc ctgcctgaat taatgccaga ttttggttta atcctatact
    15421 tccacttgac ataggaattg tgtcgttgat actgactacc ttgttagcat tgaaagacac
    15481 attcaacatt gtattccatt tcaattctcc cgtcaaattc cttgtattta cagtcaattc
    15541 aataccttta ttctccattg ttcctgcatt gatagaagga acataaacat cggaataacc
    15601 tgttgaaaca ggaactacca taggtactaa cattttatcg gtttgcttca gataagcatc
    15661 cagcgttact tctacacgtt gattgaacat tgtggcatct aacccaatat ttccttgttt
    15721 ttgttgctcc cattgtacat aggggttagg cattacagtt gggactacag cagatacaac
    15781 gttgttgtta aagttgtatt ttatcgtatt caatgatgac gcaaatgagt aattaccgat
    15841 actttgattt ccagtagcac cgtatcctgc acgtattttc agataattca cgaaagtaac
    15901 cggtttaaag aaatcttctt ctgaaatacg ccaggcaacc gaagctgaag gaaataaacc
    15961 atatttattc ttttcaccga aacgggatga accgtcctga cgaagtgttg ctgtaaatag
    16021 atacttatct gcaaaggcat aattcacacg acccatatac gacaacatag aatagcttga
    16081 agtacttcct ccaacttttg gcaataaaat accattattt aactgctggg ttacgtcgct
    16141 ggcaaaactc tgaattgaac cattcatata gtcgtatttg ttttcttgag cacttgtacc
    16201 agccataatg cccagtttat gtttgccaaa cgttttgtcg taggtaaagg tgttatccca
    16261 caaccacgta agatttttat taaactgttc acccaggtaa gattcaagat ttacactggt
    16321 atcccaagca cgtttaggtg accatgtacg cgagtcccag aaatttgcct gtaaaccaaa
    16381 ggtggatttg aatttcagat ttttcagaat ttcaagttct ccataaacag agcccatcag
    16441 attatacccg ttagttgagt tttgaactgt tttagctgca cctatcggat tagtaatatc
    16501 acctgaataa ataggttgac caactgggcc tgtataactg ccatcttcgt gataaatagc
    16561 ctgcgttggt aatgccagca ttgcattgcg gatactgtaa tccccatttg atttgatatc
    16621 atgattcaat gtcagactgt taccgaattt aaggaatgaa gttactttgg tgtcggtatt
    16681 taactggaaa gtaaagcgtt taaagcctgt atttaaaaca ataccatctt gattgaaata
    16741 attaccagat acatatatat tggttttatc agaacctccg gaataattca atgagtaatt
    16801 ttgcataggt gccgtacgga aaaatgcacc taaccaatca gtnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nncattcgac aatgcaaatg tatacgcatt ttatagcttc
    16981 gaaatgagtt gaatttctta aagtagatag agaacttagc caaatgatac aattagctaa
    17041 cagatagcaa tttaggcttt gtttgctaga aaacaaagta gatgtcttga tataaaaaaa
    17101 gatgttctaa cgacaaagaa agcctttcaa aaccttttaa atatgtgcgt tttctattta
    17161 tatactcata cctgcactac atatatttca atcttgatgc gtccaacttt cgacttgtta
    17221 ctgaatcttt ttctgcaatg atataattgg tcagtagaga agtatcatat ttccttgcaa
    17281 ctaaataact gatagttgaa acaatttata aatgggttaa ttcgggggtt tctctaaaac
    17341 aataaaccct gaaagcaaga actttcaggg tttatatcaa aactgttgta tcaacatcag
    17401 ttatttttca ctaactttaa aacaatacaa gcatgagcac ttagtgaaat agaataagat
    17461 gccatagatc ctaaatcctt atgagaccaa aggtctcgga ctgaagcaga gctaatacct
    17521 aatgtgctaa aatctattga ccgattgatc gaataattct cacgattaaa caaagcaata
    17581 atccaatcgc catttgtcat ttgtcctttc caaatttgac tctgtgggtt ggtaggatca
    17641 ttcgaaagtg gtttcccaac aaaaccatct ttatttaatg caagcatttc ttcattctga
    17701 tatagccaga ggtcattacc aatcgtattg aattggtcag aaatagttac aggacctcct
    17761 gctactagat gtaatgaaat tacagatttt ttttcatcat tatttgcaaa agtattaatt
    17821 cggataaaat cgccatccaa aatcattttc tttcgaccag agagtctcga ccaataaata
    17881 taaccatcga atgcagtagc atattgagac caatgttgtc tgtgttgtcc acgtgcatta
    17941 tcactgaatt tccaccaacc accatcacca gtatcttcgt taacacgaat catgtgtcca
    18001 tatttatttt caacctcagc atcgttgtat aagtttggca ttaccaacga aataaagact
    18061 ttatatttgt cagcagactc acgcatccat cttaatgctt tttcataatt ctcttttcca
    18121 tgggctgccc caacagtacc catccattga tcgtaacccg tttcatacca agataaaaag
    18181 tcgattctta gatagttaac acccatttgt gagtaatact taaagaaacc atcgacatat
    18241 tctcttgctc ccggtttatc cacttgcagc caacgaaatc cagaggtaga ttcatcccaa
    18301 ttaataaggt cagagaggtt aaactgcgtt ccccatactt ttatacccgc attggctgca
    18361 gcaggattta cccacaatgg gttatcgtag atacctaatc tcatccctct tgattttagc
    18421 tcttttgacc accaagcata atcatgtgtc cagttacttg aatgtgattt gcgataacct
    18481 gttgtaggat catataccat atttccccaa ccatcaatac aaaccatgtc atatccatat
    18541 ggtaataagt ttttttctac ccaattaata ttgtcagacc acacattttc agggatgtag
    18601 cctatatgag tgttctgttc atcttcaatg ttatattcgt atggactcca atataaaggt
    18661 gctttatacg gattagcagg tgttacccaa ggtgtatcaa ccacctcgtt ctcagtgata
    18721 tttggtttcc attcttcttt ttggctgcaa ccggcagcca gaaagaagaa tggactataa
    18781 atcaaaaaca aaactttctt cttcattata gctttttaaa tacaactgtt aattttgctg
    18841 gatttacagt tattttatac gttccagcag atttaattat ccactttttg tccgggtttc
    18901 caccagctac aaattgcatt gtagtaatat cagtagcatc gccattaata attgtaccat
    18961 tagctatcgg catcaaaaaa tcacaccccc aattacctgt agaaagtgga aatttcattt
    19021 ctcctgctaa taaactacct gtaaatgtga atacctcagg attttttacg tcccaattta
    19081 attgaacagg agcgtttatg tcccagcccg ctgttgtagc tgcaccaacc atataaattt
    19141 tttcataagg agtatatgcc agtgcatatt ttaacgttga acgattcatg tagaaagtat
    19201 agcgtccact tttagatatt gaaaataagc tatctttgtt ttcagcagat gttctgtaat
    19261 agagcagact atcacttagt gggttaagtg atggaaattc gttgctgatg ctttcgatga
    19321 ctttaaaggt tccaacgcta aagtttcctg tataagaata ttgttttcgt tcaataattt
    19381 ctgttagttt aactcccttg ctgtaatccc aacctccaga aatggcactt ccaactaaat
    19441 atagtggcaa tggaccaagt gaaaatcctt ttgcatagaa cgaaaccgat gccaactccg
    19501 gtttttgata ttgtttgcta ctatcaacac gagcaataat ttcagcatca attttcactg
    19561 ttgaacctcc aggtataccc caatgttcca ataaaagagt atttaactct ccaacagtga
    19621 tagacttcga gaaaatacct ggttccattt cttctacatt ggtggctgtt tcaaagcttt
    19681 ttccggctat atccattctg aaaaggtatg tcaacttagt gcctgcacct cggtcattac
    19741 ctttgttcca agtaaatata acagcagtgt ctttttcatg tccttctttt aaaattatac
    19801 tatctgtaga tgcaactaca gtcattggct tctcaaaaac cggattctga agccagtttg
    19861 aaacaattcc ggtatcttca catgcactta cacaaattgt caagagcaat aaaaggatta
    19921 ttttttttat cgttttcatt ttattagata taaattatta ttcccaaaag ggagcttgaa
    19981 ctaaatttgg atttttttct atttctgata aatgtaccgg gaaccaataa aatgctttct
    20041 tataaacacg agtctgatat tgtgttctag taaagaaatt tgtttggttt gtaccataga
    20101 aattcatacc ataaaaattc tgattcaata cactctcagc ttgtttccat cgcctcaagt
    20161 cgtcatacct gataccttct gtacaaagtt ccaccctgcg ttctgcgcgt attaattcac
    20221 gcattgctgt ttggtcgcca agatcagcat gaatcaccgt agtacttgtt accccagtgc
    20281 tatattctct aacaccagca cggttacgga ttttgttcag gtataataat atgtcaggat
    20341 ttccaggatc tatctcattc aacgcctcga tatagtttag gtacacttca cctaaccgat
    20401 acaggatacc aggacgccat gcaaagacat tattgattgg atctcttgat ggatcagtcc
    20461 tttttctcga taaatatcca ttttgaggag catcatgtgt accgggatta tctttaccat
    20521 tataataaaa gtttaaggtt cttgagtctt tattatacca ctgtccattg aaattaacgg
    20581 ctaaataaaa acgaggttca cggttgcaat acattttgta agtgccagct aatgtaacct
    20641 gacctacatt cttacactct ttccattttg ttttaccagt tgtaaaataa ccatcagacg
    20701 tggaaaatcc ggtttcagag taaccagaat ttgggtcatc cttaggtaaa ccatttgcca
    20761 tgaaaaatgc atcaaccaat gattgggtaa caccgtatcc accatttcca gaactaccat
    20821 aaggtgcaga atgcttgtcg tatgtcgagt aatcacaact tggacgtgca aatagaattt
    20881 cggtatttcc tttactatat tcttttaaaa ataaattttc acaagataag aacggatcaa
    20941 tactaccatc gtcgttcagc tcagtgtata atgcatgccc agcagcctct gcatcatcaa
    21001 tcagtttttt agatgcagtc gctgcttttg tccattttga agcatcatat gttgtattaa
    21061 aaagttcttt tccttcttta ttggtatgcg ccacataatc aggatttcca tttacaagtg
    21121 gactagctgc aaaaagcaac attcttgcac gtactgcctc acacatgata gatgtagccc
    21181 gtccatattt acctccatca ctgtatgttg ccggaaggat tttagcaacc tcagttaatt
    21241 cagtgtcaat ccaatttaca atatcatcat atggggtttg accgaccatt aaatcagaaa
    21301 gattataatt tacaggtgta atataatcgg gtttaaatgg aatcggaccg taagtattca
    21361 ataataacga gtaatagtaa gctcttagga aacgtgcttc cccaaccatt agggcaacct
    21421 cttcctgcga aagtttttga tcaggcaagg cctgtacgtg ttgcataaat atattgcatt
    21481 cacgtatttt ttgtggtaat actgaccaat aacttggact ccagtctgaa tcaattgtcc
    21541 attcacctag ggcaaaggga atgacctgcc aaccataatt acgccatctt tcagatggag
    21601 acatatcatc acctaatatt tcccaaccta aatcgcgcat atacccccaa tacggatctg
    21661 gtatcattga gtaacaccca gccaaccatt gctgagtttt cgttttgttt tgaaaaacac
    21721 tctccagcgt aatttccgta tctgcttctt tgtcaagaaa atcagcacaa gatgataaca
    21781 gcattaaagt gaaaagcaaa aatgcaattc ttatatttga ctttttcatt attatatatt
    21841 attaatttta caagcttact tgaataccaa aaagaacaga tttcattgag ggatatttta
    21901 aaccggtaga agttgccagt tcggggtccc aaagattaaa agaagacaaa tagaataaat
    21961 tatttccact gagataaatt cttactccag acaatttagc tttttcaatc aaactcttct
    22021 ttaaagagta tcctaattca agtgttttta gtcgaagaaa actcctgtct tttttccacc
    22081 aagttgaagg tgctgagtta ttatagttgg ttccataatc caatcttggc caaaacacat
    22141 tctgacttgg gttttcttct gtccatcgat cattgtaatt tgaataaata tttcctagta
    22201 caccttggcc acttccgggt ataaaaaatg atgagccaga accttgacca attatctgat
    22261 atgtattggc aactccctgg aagaagaaac tcaaatcaaa ttgcttgtat ttagttgtat
    22321 tgccaaatcc atatacaatc tgcggatcca atgttcctcc gatataacct tcatccaatg
    22381 aattaataat accatctttg tttttgtcga tatatttaat atcacctgga cgaacaggag
    22441 ttccaccaat ctgaggtata gcttgtccac ttaccaaatt accacttcca tcaaaatcag
    22501 ccgatgtata aagccgatct gctgtgtagc cccataaagt gttatttggt aatccagtag
    22561 ccgaacgata agttccaaga actgcagccg cttcatcttt ttccaatact gtgtttttag
    22621 cataggtaaa gtttgctctc caattcatga aaagagtttt ggtgatttgt ttattcattg
    22681 acaatgctac ctcaaagcct ttattgttca tttttccata atttgcatac ggagtagaaa
    22741 ggaatccagc ttgagttgga actgtagtac gttgaataaa aatatcttta cggtcttcat
    22801 taaatgcatc tacctgaaga tcaaccattc ctaaaattcc taattcaata cccacattcg
    22861 cttttgctac tttttcccaa gtaagattat taactccaat ttcaccttct tgaatgccgc
    22921 tgtaatatga accatttcct gtgccccatg tatatccaac aacgttcgtg tttagagttg
    22981 tcaaatatgc gaaacggcgg tttgaaccaa tgttatcatt tccaacaaaa ccatatgatc
    23041 cacgaaattt aatcttgttg aatatttttt tgtaaggttg catgaatttc tcttcgctca
    23101 acagccagcc caaggcaact gaggggaaaa ggccgaaccg ttgcccttta gcaaaatttt
    23161 ccgagccatt atatccgaaa ttaaattcac ctacataacg ggtatcatag atgtatgata
    23221 gtcttcctgc aacaccctga tgtctgtatg gttgtatacc accattgtca taactgttct
    23281 gattatacag taacatggca tcaatatcgt gcttcccaaa agtgcggtta taatataggt
    23341 ttccctctaa ataggtacca cggttacccc aatcggcatt tgttgacagc cctaagtatt
    23401 cttgtccgta tgaataaatg gaatgaatta aatttccttc cacgtcacga cccgttgcta
    23461 cattataata atttggtgat ttgccacgat ttacgtaatt agcagaatat gaatcaaatg
    23521 agaatgtaaa ttttgctttt agcccgggag taatcatttt taaatcttgt tcgagcgaga
    23581 ataatgattc aattttgctt tcccctttcc tgtaatatcc ggtttgggta ctataagccc
    23641 atggattagt tctttgagct acaataggga atgtaccatc tgaataaact gtgggatgta
    23701 cataaggtgg tgtttcaaaa gctgcagaga ataaatcatc agtactattg agtccttttt
    23761 ggagttcttg taaataaccg ccaatattca cccggagcaa agttgttttg gataggttta
    23821 tatcaacatt tgaacgtata ttataacggt gcatacgact ttctgagcta taagtctggg
    23881 caggatcgcg tgctataatc ccattttcac cataatagga tgctgtcaat gaatatcgca
    23941 aaatatccga acctcctgca acattaaggt tcgtccgtgt attgctcgca taagattttg
    24001 ttattgcatc aatccaattg atatttggat acaagtccgg atcatatttg ttgaacgttt
    24061 ttacgattcg atcatttgaa aacggtttgg tgtaacctgc attatttgct aactcattca
    24121 gcaaagtcat atactctgca gctccaatga acgaaggaag ttgggttggt tgctgaacag
    24181 attgttctgt tctgatttca attttaggtt ttcctacaaa tcctcgcttt gtatttacaa
    24241 ggataacgcc attggcacca cgaactccat acacagcgct tgcggcagca tcttttagta
    24301 tggaaaacga ttcgatttca gcagcatcta aatcattcaa tgaacgttct attccatcaa
    24361 ctaataccaa tggactagtt gatccagcaa aagtagaaat tcctcgaatc cagatatcgg
    24421 atgcgtcgta acccggttcg cctgaacgct ggacaccaat aaccccagca agttgacccg
    24481 caaggttatt acttatgtta ggcgtagtac ctacttgtaa taatgccggt gtaacagatg
    24541 ttattgcacc aacaacagaa gctcgtttct gtgttccata tccaacaact actacttcag
    24601 aaaggtcttt tgaagattct tctaatgtaa tcttaaaatt gctaaagacg ttgggtgtaa
    24661 tttcttgttt cacatatcct atgtaagaaa ctacaagagt aggagcagtt acggctccaa
    24721 gctgaagagt gaatttccca tccacgtttg taattgtgcc ggtatttgtt cctttaagta
    24781 ggacacttac accgatcagc ggttctcctt tggtgtcgac aacgataccg gagatagctt
    24841 ttgttgtagt ttgggcaact acattaggca agacaaatag catactgacg aaaaacagca
    24901 cactaaaaag gctttttttc aaattgacca tcatttaatt tttaaggtta ttaatatata
    24961 caaaataaat ataattcaat gatccgcata ttgaattaat aatttcattt tcaacagcac
    25021 aaatgtatgt gaatcttacc ttcttaaaat ggcataaatt tcttaaagta gattgaatat
    25081 gaaattgtgc agtataatgt cctgataaaa aacgacatat atattttatc aaataaaaca
    25141 aagtagtctg tttgaaaaaa aaaggttctt ataaccatga tagttataaa aaccttctcg
    25201 aaaatctatt ccctcaatta tttaaatact cctatttgca tcacttttgc ttcaaactcg
    25261 ttacgtggtc ctttggattt atttctgaat cttgttcgac aattgtatat tgttgttgct
    25321 gaataacgca aaaagaaaga tattttatcg ctatctttaa tcccaagccg tataagagca
    25381 aatactcgca atccggtatt gagaatttca ccagatttta gtttgatttc ctcatcatca
    25441 atcattagtt ctcttaattc ttcaatgaaa ctaggaaata actgcatgaa tgtactatca
    25501 aagtttgaat aaaattcttt aagttcttcg tcaattattt ttgtagactt aatctcattt
    25561 aataattcgt cttttttacc agcaattgat attttattta aatgacgtcg atattcatct
    25621 aacttgttga gatatacgga acactgatct atatacctgc caatatacac ttctttgatt
    25681 aaattagatt cttgtaatgt ctcgtttgtg tgtttaagtt gattgttagt agaggataat
    25741 tcttgattga gcgagattaa ctgctcgttg gccacactca ggtttttccg agcaactgcc
    25801 aatttcttca tttgcctata gaccaagaat attgccacta taagaaggaa tgatagtaca
    25861 ctaatactgg aaagggagat taacatcaac cgttggcgtg attccgtttg ttgttggtaa
    25921 gctttatcaa taatcggcat cattttagat acttcaaatg tccttagtct tgcattacaa
    25981 aaaagagcgt cgtccagtga gcgcttcata tatttgtaag ctctggttac atctccctct
    26041 tcgtttaata tgaatgccag gcgacgtaat gagatatagt ctttggtagc tgattcgata
    26101 tcgtggatag ctgaaactgc caaccaatat ttttcctcag ttttgttttt ttttctgttg
    26161 tatgttagcg agatgcaata ggcaattatt gccttgtcat gtaaattgtc cttaatggtt
    26221 ggataataat taaatagtaa cgttgacgct tcatcgtatc tcttttggga aataagttcg
    26281 tcggatttta ccattatgta tggacctgaa tttggaataa tagtttgtag taaggaatca
    26341 cggtaacttt cagctatcac ttcgtatctt tttttctcct ggctcgaaac cgtataatca
    26401 accatgaagc catagaccgt acggtaaata tgaaagtaat atgattttaa gtcaggagct
    26461 tcattgatat taactttact cataatgtcc attgactctt tgtacattcc gcttacgccc
    26521 atgattgaag ccaggtttag tcgagcatct gccagattcc ttttattatt cattttcttg
    26581 gctatctgta gtttttttct ggcataaaca agtgccgaat ctgacttata ggaactatat
    26641 tcatcatata gcttaccaca aatctcatat ttttggtcat acgatgttgt atatctcaac
    26701 aaatctttca tcttgttcat tttctcttct ttcttatttg aataaacctg ataattatct
    26761 acggttctat ccaatacttt caataatgag tctatgccat tgtctgcaaa gagtgagttc
    26821 gataatacaa aata
//
LOCUS       DAOPYB010000022        26517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1808_length_26517_cov_8.976306, whole genome shotgun sequence.
ACCESSION   DAOPYB010000022 DAOPYB010000000
VERSION     DAOPYB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26517
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1808_length_26517_cov_8.976306"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PKH58_03180"
     CDS             <1..1122
                     /locus_tag="PKH58_03180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX88062.1"
                     /translation="VISTFFSVYPFFENQFGHFVSKGYEIHLALSPSEEIEEYANEQQ
                     IKYFETPINRSYSLFDDLKSIHAIYRYIKKNNIEIVAGHSPKGALIAMISSYLAGTKR
                     RIYFRHGLVYETSKGLKRCILIFVERLTSFFAKEVVCVSPYLIEKSINDKLTRKSKLK
                     ILNIGSCNGVDALKRFNPANIQSDKKQTLKTKLGIQEEAFVIGYVGRLAKEKGINEII
                     KTFSLFNQYNKNSYLLLIGPFDDRDVISNETFRKIQKGKNIIYPGEIYEDLEYYYSLM
                     DVFLYPSYRDGFGNAIIEASSMELPVLTTSFSGNRDAIRNGITGKYIGLDPNEISLKI
                     EDYYNNKELAKIHGRNGRNFVLENFSNELIWKYLDNLYN"
     gene            1163..2122
                     /locus_tag="PKH58_03185"
     CDS             1163..2122
                     /locus_tag="PKH58_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX88063.1"
                     /translation="MTYFIILILLFVAELLYFRIADRYNIIDKPNERSSHSYVTLRGG
                     GIIFYIGVVFYFILNQYAYPWFFAGLTLISIVCFIDDVGSLPSLFRLCIQILAMGLMF
                     YEWNLFAMPWYVWLVALILCTGTINAFNFMDGINGITGGYGIVIASALWYINEKIISF
                     TDENLIVILILSLAVFNVFNFRTKARCFAGDVGAASVAFILIFLLGRLIIQTGDFSYL
                     VLLALYGVDTVLTILHRIILKEPLMVAHRKHMYQLMANELKIPHIVVSLIYMFVQAVI
                     IAGYFITDNRYMYLFLVVAMLSAIYIIFMLKFFRLHSENNRYE"
     gene            2226..3359
                     /locus_tag="PKH58_03190"
     CDS             2226..3359
                     /locus_tag="PKH58_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNX88064.1"
                     /translation="MSKKVFVSGCYDMLHSGHVAFFEEAASHGDLYVGIGSDKTIADL
                     KARKTVNSDAERLYMVKSLKVVKDAWINSGSGLMDFLEEIKKLKPDIFFVNTDGHTPA
                     KEELCKELGIEYVVSKRIPHVGLPERSTTALRQVCRIPYRIDLAGGWLDQPYVSKHCP
                     GAVLTICIEPDYEFNNRSGMSTSSRNKAIELWQTDIPEGDKEKLAKTLFCFENPPGTS
                     YVSGSQDALGITMPGLNRFYYEGGYWPVKIENHSEPDLLDWIEERLWLIPLFPRQEKY
                     DVLARTYITEEKACALSRAADECWNALLIKDAIALGNAMTSSFNAQTAMFPDMVNNEI
                     ITQLEHYKADVLGWKISGAGGGGYLILLSEKSINNAIQIRIRR"
     gene            3490..4830
                     /gene="fucP"
                     /locus_tag="PKH58_03195"
     CDS             3490..4830
                     /gene="fucP"
                     /locus_tag="PKH58_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HNX88065.1"
                     /translation="MSKSLFNTQSGKNYLFPFVLITSLFFLWGFAHSLLDILNKHFQN
                     ALDLSKAQSGLVQASAYGAYFIMALPAGYIARKYGYKKGVLFGLLFFIVGCFWFVPAV
                     GIDKFWAFLLGLLIIFSGLTFLETVANPYTTVLGDPKYAASRINLAQTFNALGWIAGP
                     LVGSLFIFKNTSTDSPLVSFGKTLKVIFTGIAESSAQVATEGGKIVDNSALIIPYVSL
                     GVVVVLVAVMFFLAKMPEVNPEAETHPTGLNETSEDKPKIIHRPLIRQQHFVMAVVAQ
                     FLYVAAQTGIGSFFVNYTVETKNLGIGEMQAGFLLGLGGMGLFATGRFLGSFLMRYVK
                     PEALLGSFALINTFLMLVTANRHDNLGIIALILSYLFMSIMFPSIFALGIKGLGPQTK
                     TAASVMVMTIVGGAICPSLMGLIGEHNMNVGFIIPMICFAYIAVFGFVQSKARR"
     gene            4856..5935
                     /gene="gmd"
                     /locus_tag="PKH58_03200"
     CDS             4856..5935
                     /gene="gmd"
                     /locus_tag="PKH58_03200"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNX88066.1"
                     /translation="MKKVALITGITGQDGSYLAEFLIEKGYEVHGIIRRSSSFNTGRI
                     EHLYFEEWVRDMHKQRTINLHYGDMTDSSSLVRIIQTIQPDEIYNLAAQSHVKVSFDV
                     PEYTAETDAVGTLRLLEAVRILGLEKKTRIYQASTSELFGLVQEVPQKETTPFYPRSP
                     YGVAKLYGFWITKNYRESYGMYAVNGILFNHESERRGETFVTRKITLAVARIAQGMQE
                     KLYMGNLDSLRDWGYAKDYVECMWMILQHPEPEDFVIATGEMHTVREFCTLAFAEAGI
                     EINWEGQGVDEKGIDKATGRVLVEVDPKYFRPAEVEQLLGDPTKAKTLLGWNPRKTSF
                     GELVRLMVEHDMRFVRKLKLRGEME"
     gene            6095..>6190
                     /locus_tag="PKH58_03205"
     CDS             6095..>6190
                     /locus_tag="PKH58_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX88067.1"
                     /translation="MEKTSKIYVAGHRGLVGSAIWKNLKSKGYTNL"
     assembly_gap    6191..6290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6291..6831
                     /locus_tag="PKH58_03210"
     CDS             <6291..6831
                     /locus_tag="PKH58_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX88068.1"
                     /translation="SHVLPAMIRKVHLAKCLQDNNFEAIREDLNRRPVEGINGETSDD
                     KIMAILKKYGIKKGILQLWGTGTPLREFLWSEEMADASVFVMEHVDFAQLSEGKKEVR
                     NCHINIGTGKEISIRELSALISQVVNYHGEIRFDATKPDGTPRKLTDVSKLNSLGWKH
                     KIEIDEGVKRMYEWYTGKS"
     gene            6888..7283
                     /locus_tag="PKH58_03215"
     CDS             6888..7283
                     /locus_tag="PKH58_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNX88069.1"
                     /translation="MEIIDTTLLDEVTRQAKESPRLRMNYNLHDSLEAKAQKLLNALE
                     QGTQLPIHRHQHTSETYILLRGRLKIIFYNEQKEIVDTKLLDISEGKFGINIAAGQWH
                     TLEVLESGTVIFEVKDGPYNPVNPEDVLL"
     gene            7364..8881
                     /locus_tag="PKH58_03220"
     CDS             7364..8881
                     /locus_tag="PKH58_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNX88070.1"
                     /translation="MLKVYKPKLNLILTDLILISVNILIVFNFFPLTTQNPISKYFIP
                     VNLTLLVWLLVSYLFGRYRSLKTVSFIKSAFSLFFTSLFILVFFGGYIFIQPASPYSQ
                     NVLITIVLGVFVSLYFFLLVYYLYKYAVQYDTLNFNPDKRLSAIPHPSHSLSDDAVVE
                     RRMRITEFSGEKVLAFLEKNTRLYESGTCVFTEFSSKDFESVEHYQYSTFIQLKRLNH
                     IRGINKMLAMVNEKLPDDGMFVCCYKSQSTIKQMIYHKYNNLIANSIYIIYFLYHRVM
                     PKFFLTKRLYYDITDGKKRVLSKTEVLGRLNYCGFILERQFKINDMNYVIARRVKNSE
                     PLLDRNYGALIKLKRKGKDGKIFSVFKFRTMHPYAEFLQQYIYENNDLQEGGKFKRDI
                     RITTLGRFMRKYWLDELPMLINLVKGEMKLVGVRPLSTQYFNLYSPELQEKRTKFKPG
                     LLPPFYADIPKTLNEIQTSEMKYLTECENKGIVRTDIKYFFLIVKNILIKKARSA"
     gene            8949..10655
                     /locus_tag="PKH58_03225"
     CDS             8949..10655
                     /locus_tag="PKH58_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88071.1"
                     /translation="MVKKTILILNILFIAVISFAQNIPQHISYTRIYDFVDELANDGF
                     FELNSAVKPYSRLFITEKLLEAKQQEAKLNVRQKRELHFFLEDYTTEQDRLPEFEFPI
                     IENENFRLDLYPPIFNYKDTLFRARIQPILGMNIYSNKQGRITQRWFGVDFQSMIGKN
                     LSIYGSLRDISFSGGRLSEPSYLTNFPGFEYKEPSDFSDSRGGITYGWKWGSIGLIKD
                     NIVWGDNYNGSNIISGRVPSFPMINLSVKPARWFEMNYIHGWLVSNVVDSSSYYMENG
                     VKKWYRNHNKFIAANIFTLTPFRNFNFSFGNSIIYAENNVQPGYLLPVAFYKSIDHTM
                     TKGIATENQNSQMFFNISSRNIRHLHLFSSVFIDEMKFSRFKPSSAEKNPISLKLGAK
                     VNNFLIDNTSFTAEYTRTNIINYKHSIDVLTWASNDYNLGHYLGDNAREIYFSAGYKP
                     IRGLDISLSYLNAKHGNEYNYLRRENNQAVINKIISQPSPGDVIWKNQTLGLKLVYEL
                     FNNGYAVINVENSNIQGFEAQSEPITGERRMTAQEVLNYYTPAFLQGKNTTLTVGFSF
                     GF"
     gene            10734..11333
                     /gene="coaE"
                     /locus_tag="PKH58_03230"
     CDS             10734..11333
                     /gene="coaE"
                     /locus_tag="PKH58_03230"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNX88072.1"
                     /translation="MKTPLIIGITGGIGSGKSTFSRHLRELGLQVFDTDMEAKNLQNF
                     CPVVRNKVIAEFGEGVYNANGLDRAKMAQIVFANADKLQILNNIVHPEVKKKFYEWVM
                     IHPEERFLFMECAILFEGGFNELVDKIVLVTAPIEERIRRVMNRDNVTAEHVMARVRN
                     QWADEEKKRLADWTFDTNNDIFPSARVHDFLSMLEHTLL"
     gene            11410..12252
                     /locus_tag="PKH58_03235"
     CDS             11410..12252
                     /locus_tag="PKH58_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX88073.1"
                     /translation="MTEKLGSICGEHIRVLEQMVPVDVFVKLRTVSFYSPANNSNPPV
                     IFLGGLSTVVESFTSIIRDLTKDFNFHAVETRDHASSQLGENQKFDMETMGRDLGEVV
                     KFLNLTNHQFILLGYSLGVAVIVEALRFMTNKPLKMIFLEPTTVFHYPSWSIPVIKLS
                     LLFKTKRLRFIAKWYINNFVIDKKADAEMAEISSNAIDNADTQKLKRAILAIAGYEIW
                     EKIEHIDCPVLIISASKDKMHRNDDILRLNSLITDCNIIDLETNKRSHSMEASQIIRN
                     FILT"
     gene            complement(12305..15451)
                     /locus_tag="PKH58_03240"
     CDS             complement(12305..15451)
                     /locus_tag="PKH58_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HNX88074.1"
                     /translation="MKCNTKIIRTLITACCFLSVSNLNAQLVTSANKDALARISYNIP
                     INRDVVRKFQYLPEGNAFVCINGNNRFTRSLYGTNTESRLETSDRPVFASYYKSADSS
                     RNIRFVINNGNKSLWLDSAEYCKAGYLAGKRYYELRDKLFSKGKLHITALALAENEGA
                     VWKIETENFPKNFNLVSVISKIKADKLKHLGEMGTNPPDVLEAGNKLGDVKVNFTDKK
                     AYLLLEGAELQQIDNKRGEIIYLKAESCAKKISEQITIRTPDKYINTVAGALSSAADG
                     VWDGKNAWLHGAIGWRMPLTGWRAAYTGDFLGYTDRARKHFDAYAASQVTSVEPTVSH
                     PSQDTTLHLARAEKKWGTQMYSNGYICRSPFNSNQMHHYDMNLVYVDELLWHLNWTGD
                     LNYARKMFPLLNRHLEWEKRNFDPDDDGLYDAYCCIWASDALQYNSGGVTHSSAYNYR
                     ANKITARIAELINEDPTPYRDEARKILKAMNDKLWLKETGCWAEYVDFMGYKKTHPSA
                     ALWTVYHSIDSEVGDIFQQYQTTRYVDREIPHIPVKADGLNGTYEVLSTTNWLPYAWS
                     INNVAFAEIAHTSLAYWQAGRANEAFRLFKSAILDGMYLGRSPGNVGQISFYDAARGE
                     TYRDFGDPVGIYSRALVQGLFGISPDLLNGRVIIRPGLPRDWNFAEFTAPYLEFNFRR
                     KNKTDYYLLKNKFAVKSELILRLPVTHEKIKSVKINGKTASWTILENIEFPLVEITCG
                     NAEQPEIKIEWEGNQLKPSVAKTTGKRDGFVKLLQREMSWWQPTEAETRKQDHSFAKN
                     TLLPDFLKEKSEFIQINIDSVLNASVTDIFKNKYLSPRSPYTTLQTPWQGVGEWCKPL
                     LTYEIDDTGLRRSTRNQTFTTPMGLPFRIPDGKNNIAFTTLWDNYPDSLSVTLSGRAQ
                     HAVLLMAGTTNPMQSGFVNGTVTVSYKDGTSETLQLINPDTWCTIEQDYYTDGQAFRI
                     NTPRPYRVAFKSGIVSSDIGNALNIDSMEVYGRVIDGGAGVILNIPLDKNKELQSLKL
                     KSLANEVIIGLIAVTLVK"
     gene            complement(15554..17596)
                     /locus_tag="PKH58_03245"
     CDS             complement(15554..17596)
                     /locus_tag="PKH58_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNX88075.1"
                     /translation="MTKTFIFSSFRKHQSGISLKMMLTVAFIYTSQLTFSSQPVVDYN
                     IQGIPFNTVSLNDHFWLPKIEINRTVTIPSSFRKCEETGRVENFILASGAKKGKFLTV
                     YPFDDTDIYKTIEGASFSMSVHPDKAMDHYVDSLISIVAAAQEPDGYLYTARTIDPKN
                     THSWAGSERWVKESELSHELYNSGHLFESAAAHFTATGKRNLLNIALKNANLLVQVFG
                     PGKRDDAPGHEIVEMGLVRLYRITGEKKYLDLAKFFIDCRGKNSKQNYSQNHLPFVKQ
                     TEATGHAVRAGYLYSGAADVAALTGNKEYIEAIDKIWENAVTKKFYITGGIGALHAGE
                     AFGADYELPNLTAYNETCAAIANVYWNYRMFLLHGDAKYIDVLERSLYNNVISGVGLD
                     GKTFFYPNPLECNARYAFNSGNKLTREPWFDCSCCPTNLCRFIASVPGYIYAQKTDKI
                     YVNLFIGSKTRFKLPKTSVEISQQSDYPWNGNIALQVSPASKSVFSLYLRIPGWAHNQ
                     PVPGDLYRYVSEGAEEIEIKINGKKVEFKTQDGYAVISRTWKKGDRVEYTMPMNVHRV
                     EANEKVQDDRGKVAIERGPLVYCLEGIDNNDIVDNIILPDQAPLSVSFNPNKLNGINE
                     IKGEAVVIKISPDGLSAQTGKQNITAIPYYAWSNRGINTMKVWLPRKIKELKIINE"
     gene            complement(17945..18385)
                     /locus_tag="PKH58_03250"
     CDS             complement(17945..18385)
                     /locus_tag="PKH58_03250"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HNX88076.1"
                     /translation="MKKSLILLMIMAGITACVQKSENTSEQPVKDSVTVITENRADVQ
                     SDSWLGQYAGFFPCGDCEGIETVITLQEDSTYIQKSTYLGKDNSFEETGTFTLIEPGS
                     RLVLKSADSEQSYLLGDGTLTLLNSEGKVTEGELADAYVLKKKN"
     gene            complement(18404..20452)
                     /locus_tag="PKH58_03255"
     CDS             complement(18404..20452)
                     /locus_tag="PKH58_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNX88077.1"
                     /translation="MKKLILFLMISSLIACGGNKQKENATPSVEDFNYSVDKFADVEI
                     LRYRVPGFEKLSLKQKEMIYYLSQAAIEGRDILYDQNNKYNLSIRRILEAVYENYKGD
                     RDAEPFKQLTTYLKRVWMANGIHHHYSEDKFTPAFSTSYFAETVKSIDPAKLPLQQGE
                     TVDQLIARISPVIFDPTVYPKRTNQADGVDLILTSANNYYEGVTQQEAEDFYTKMKNP
                     RDSTPISYGLNSKLMKENGKIVEKTYKIGGMYSKALSRVVGWLEKAAEVAENDKQRDI
                     INTLIRFNQTGDLKTFDEYCIKWVQDLTSQVDFVNGFTETYADPLGLKASWESIVNFK
                     NIEATKRTEIISSNAQWFEDNSPVDKNFKKEKVKGVSAKVITVAMLGGDCYPPTPIGI
                     NLPNANWIRAEYGSKSVTIENITEAYDQVAMGNGFNEEFMWSDEERNRAKEYASLTNN
                     LHTDLHECLGHGSGKLLPGTDPNALKAYDSTIEEARADLFGLYYIGDPKLVELGLLPN
                     NEAFKAEYYQFLMNGMMTQLTRIKPGKNIEEAHMRNRQLIANWVYENGKAEKVVEFRV
                     RDGKSFVVVNDYNKVRILFGKLLAEIQRIKSTGDFNGGRDLVEKYAVKVNSDLHKEVL
                     ARYEKLNLAPYKGFVNPVYTLVTDKKGKIKDVTVSYTENYVDQNLRYSREYSKLPNYN
                     "
     gene            20659..22062
                     /locus_tag="PKH58_03260"
     CDS             20659..22062
                     /locus_tag="PKH58_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX88078.1"
                     /translation="MLQNFISQRIRHELPFEPTEQQSLLIEGLGEFVMSADPEKLFLL
                     KGYAGTGKTSVVSALVRALNSLEQKTVLLAPTGRAAKVLSGYSGFPAFTVHKKIYRQK
                     SMSEFRFQLADNLHKHTLFIVDEASMISNSGIDGSFGSGRLLDDLIEYVYSGDECSLL
                     LLGDTAQLPPVEQPQSPALETGTLTAYGLKVYEFTLTHVVRQALESGILYHATQLRLD
                     LVEKEEFSHPKFELQGFADIKRLNGVELIDEIQRAYDGVGVEDTIVITRSNKRANMYN
                     NGIRSRVMMKEDELSNGDLLMVTRNNYFWNKPYQDIEFVANGDVLEIIRIGKYREIYG
                     FHFVDLSLRSLDYGWEIDARIWLESLQSENPAAMNELTNKLFAAVAEDYPELTNRKML
                     FKKILENEFFNALQVKFAYAVTCHKAQGGQWKTVFIDQGYLPEEQINREYFRWFYTAL
                     TRATGQVFLVNFPDEWF"
     gene            22182..23684
                     /locus_tag="PKH58_03265"
     CDS             22182..23684
                     /locus_tag="PKH58_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX88079.1"
                     /translation="MSKKSVFITFLALLAVLTSCKTKDPASDEVNIVSQFVYDGLSTY
                     YLWADEMKNNKPTVADSDPEVYFESVLNSLDKENGWSFITDDVQGLLSDFSGEPKSFG
                     FSLTFAAANDARTEYYAVVKYVFPNTPAARAGIGRKGIITKINGQPITENNYMLLYGN
                     STTELTLYKIADGQLKFDHIANITPETIQTNPVYYSNVYYSGGKRIGYLFYTDFISNY
                     NDSLYNTFSRFKNAGVTDLILDLRYNHGGAVTAANYLCSLIAPATVVEKKSPLVIMSY
                     NNFVNQYFDKEKWSRTDNLGAYDEKEKDPMGANLNLNKVYIIATGDSYSASELTTFCL
                     KPYMDVVHIGENTGGKFTASWTIHPYDSNIGLNIYSESSLSATEKTKLANWAMQPIVA
                     KYTNKNGADFSNPGYLIPDYPLEEGFGYISNWAAIGDPADVYLGQALYLITGEESYKP
                     VQKIGMSRVKAMRETPVKLQNPRDVRREAVILDNVKLKSLPHTQMQNIVR"
     gene            complement(23741..24109)
                     /locus_tag="PKH58_03270"
     CDS             complement(23741..24109)
                     /locus_tag="PKH58_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88080.1"
                     /translation="MIKKIILSTIILLSGFTIAGTQQQSVIREILSRTASGKVTEMQK
                     FIHFDDVQARQLEIIEYQYLLNMQKAETGCRCRIQKRTDIYNRQRDIELQKILTREQY
                     LKYDALDKDKIRKLPLRSTE"
     gene            complement(24559..26367)
                     /locus_tag="PKH58_03275"
     CDS             complement(24559..26367)
                     /locus_tag="PKH58_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX88081.1"
                     /translation="MKKNLYILAGCISVFLCYACSKSTVTPYPEPLVNAISLFYQENQ
                     NEKVIAEIDKLNSRQSTDEIKRMASLFRAAALCESEKTDSASVLFRSIQPIPDNDRFM
                     FWYNSISGLILFRQDNLVNAYEALLNATNSRYKDIRALALNERTLARIAFVLSDQHKG
                     IEWLTLSTQHFEQAGLEKGKAINLKSLGRFYMNNGSYAKALQALKTAERIISRHNDKE
                     ETFYIYIGFIDYCLLTNQPKEARYYAYKCLDLSNRIIKNSFYSLAYNNIAEVDMKLNK
                     YDEALEYLNKTLQLPSNYRTATIRQTYAHVKLSDIYLTMNMPQKALQHLLIASQSLPH
                     SGYLELKQQTYYKLSNAYRAVNNSQSAYKALETAKLYQDSTSNSLIKSIKDLYNTNIE
                     LTTTTFNLQKSRATEHKQLTIAFITIIFLVITGIITSFLYRQLHKKNNVLISLIQKNI
                     QLAEDERRLRQLQMEDNKRKNTRKVSENNDKNLQLFELLTLWLETDKNFSRKDLTLDT
                     VAKGLNTNREYLSRAISEQDIRFNDLINKYRVEEVIAIFSDPHNRSNKFNLAVIGNIA
                     GFNSNSVFIDAFRKQTGMTPAQFRSNLNKQSDRRKF"
BASE COUNT         7585 a         5080 c         5658 g         8094 t
ORIGIN      
        1 gttataagca cttttttttc ggtatatcct tttttcgaaa atcagtttgg gcatttcgtc
       61 tcaaaaggtt atgaaattca tttggcatta agcccatccg aagaaattga agaatatgct
      121 aatgaacaac agataaagta ttttgaaacg ccaataaaca gaagttattc attatttgat
      181 gatttgaaat caattcatgc aatctatagg tatataaaaa aaaataatat cgaaattgta
      241 gcaggtcata gtccaaaggg agctcttatt gcaatgattt catcatatct tgccggcaca
      301 aaaagaagaa tttatttcag acatggtctg gtttacgaaa catcgaaagg attaaaaagg
      361 tgtatattaa tatttgtgga acgattgact tctttttttg ctaaggaagt tgtgtgtgta
      421 agtccttatc tcatagagaa aagtataaac gataaattaa cacgaaaaag caaattaaag
      481 atattgaata taggatcttg caatggcgtt gatgctttga aaagatttaa tcccgcaaat
      541 atccaatctg ataaaaaaca aactctgaag actaaattag ggattcagga agaggctttt
      601 gttattggtt atgtcggacg gttagccaaa gagaaaggga ttaacgagat tattaaaaca
      661 ttttctttgt ttaatcaata caataaaaat tcttaccttc tgctcatagg tcctttcgac
      721 gacagagatg taatatctaa tgaaacattt cgaaaaattc agaaggggaa aaatataatt
      781 tatccgggtg agatttatga agatttggag tattattatt cattgatgga tgtttttctg
      841 tatccaagtt atcgcgatgg tttcggtaat gcaattattg aagcatcatc gatggaactt
      901 cctgttctta caacctcttt ttcaggaaac agggatgcaa taaggaatgg gattacaggt
      961 aagtatattg gtttagaccc aaacgaaata agtctaaaga ttgaagatta ttataataat
     1021 aaagaactgg ctaaaattca tggcagaaat ggaagaaact ttgttttgga aaattttagc
     1081 aatgagttga tttggaagta tctggataat ttatataatt aagaaaaatc agatgactta
     1141 ttttacttaa tattttttaa taatgacata cttcatcatc cttatcctcc tcttcgttgc
     1201 cgaattgctt tattttcgta tagcagatcg ttacaacatc atcgataaac ccaacgagcg
     1261 tagttcgcat tcgtatgtga ctctgcgcgg aggtggaata attttttaca tcggagtggt
     1321 tttttatttt attttaaatc agtatgctta tccctggttt tttgcgggtc tgacactgat
     1381 ttcgatcgtt tgctttatag atgatgtggg ttcgctgcct tcgcttttca ggctttgcat
     1441 acagattctg gcaatgggat tgatgtttta cgagtggaat ttattcgcaa tgccctggta
     1501 tgtatggctg gtagcgctga tactttgcac cggtaccatc aacgcattca attttatgga
     1561 tgggattaac ggtattacgg gaggttatgg tattgtaatt gccagtgcac tctggtatat
     1621 caatgaaaaa ataattagct ttactgatga aaacctgatt gtaattctga tattatcttt
     1681 ggcagttttc aatgtgttca atttcaggac aaaagctcgt tgttttgccg gcgatgtggg
     1741 agccgcatcg gtagctttta ttctcatttt tctgcttgga cgtttgatta ttcagaccgg
     1801 tgactttagt tatctggttt tattagcctt gtatggtgtg gacacagtat tgaccatttt
     1861 gcaccgtata atactgaaag aaccattaat ggttgctcac agaaaacata tgtatcaact
     1921 aatggctaac gaattaaaaa taccgcatat tgtagtatca ttaatttata tgtttgttca
     1981 ggctgtaatt attgcaggat attttattac cgataacaga tatatgtatt tgtttctggt
     2041 agttgctatg ctttcggcaa tatatattat ctttatgctg aaatttttca ggttgcattc
     2101 cgaaaataat cgatatgaat aataagtaac aagttactag ttgacaagta atgttaataa
     2161 tttctgtttc gtattttctt ataaaatact tagaagaaaa tatgaaaata tataactgat
     2221 ttctaatgag taagaaagtt tttgtcagtg gttgttatga tatgctgcac agcgggcacg
     2281 tggctttttt cgaagaggca gcatcgcatg gcgatttgta tgtgggaatt ggttccgata
     2341 agaccattgc agatttgaaa gcgcgtaaaa ccgtgaattc ggatgccgaa cgcttatata
     2401 tggtgaaatc actaaaggtg gtgaaagatg cctggataaa ctcgggaagc ggattgatgg
     2461 attttctgga agaaataaag aaactcaaac cggatatttt ctttgtgaat accgatggtc
     2521 atactccagc caaagaagag ctgtgcaaag aactgggtat tgagtatgtg gttagcaaac
     2581 gaatcccgca tgttggctta cctgaacggt cgacaacagc attgaggcag gtttgccgga
     2641 taccttaccg cattgatctg gcaggaggat ggctagatca accctatgta agtaaacatt
     2701 gtccgggtgc agtgctcact atctgcattg aacccgatta tgagttcaac aatcggagcg
     2761 gtatgtcgac aagcagccgc aataaagcta tagaattatg gcaaaccgat atacctgaag
     2821 gtgataaaga aaaactggct aagacacttt tctgctttga aaatcctccc ggaaccagct
     2881 atgtgagtgg gtcgcaggat gctcttggaa ttacaatgcc cggtctcaac cgtttttact
     2941 acgagggggg ctattggccg gttaaaattg aaaatcactc tgaaccggat ttgttagact
     3001 ggatagaaga gcgcttgtgg ctgataccac ttttcccccg acaggaaaaa tatgatgttt
     3061 tggcccgtac atatatcacc gaagaaaaag cctgtgcctt gagccgggct gccgatgaat
     3121 gctggaatgc cctgctgata aaagatgcta tagcattggg aaatgctatg acatcaagtt
     3181 ttaatgcaca gacagctatg tttccggata tggtgaataa tgaaattatc actcaacttg
     3241 aacattataa agctgatgta cttggatgga aaataagcgg agccggtggc ggaggatatc
     3301 tgattctgtt aagtgagaag agtataaata atgccataca gataaggatt agaagatagg
     3361 ttgatttgga gtttatcaag ttatcaagtt catcaagtaa aaagacagtt ttctcaatag
     3421 tttatagttt aaagtatgcg caacaaatag atacagagta ctcatgaaat aaacacataa
     3481 ccaaatttta tgtctaaatc attgttcaat acacagtctg gtaaaaatta cctgtttcca
     3541 tttgttttaa ttacttcatt gttttttttg tggggatttg cccattcatt gcttgatatt
     3601 ctgaataaac attttcagaa cgctttggac ttaagcaaag cgcagtcggg cctggttcag
     3661 gcttccgcct acggcgctta ttttattatg gctctgccgg ctggatatat tgcccgaaaa
     3721 tacggatata agaagggggt tttatttgga ttattgttct ttattgttgg atgtttctgg
     3781 tttgtacctg cagtaggtat cgataaattc tgggcattcc tgctgggttt gcttatcata
     3841 ttcagcggtc taacctttct cgaaacagtt gccaatccgt atacaaccgt acttggcgat
     3901 ccgaaatatg ctgccagccg tataaatctg gcacaaacct tcaatgcact cgggtggatt
     3961 gcaggtccgc ttgtcgggag tttatttatt tttaagaaca cgtcaaccga tagccccctg
     4021 gtgtcattcg gtaaaaccct gaaagtgatt tttaccggaa ttgccgaatc ttcagctcag
     4081 gttgcaactg aaggaggaaa aatagttgat aattcggcgc ttatcatacc ctacgtaagt
     4141 cttggagtgg tggttgtatt ggttgccgtg atgttctttc tggcaaaaat gccagaagta
     4201 aatccagagg cagaaactca tcctactggt ctgaacgaaa cttctgaaga caaacccaaa
     4261 atcattcata gaccactgat cagacaacaa cactttgtaa tggcagtagt ggcgcagttt
     4321 ttatacgttg cggcacaaac cgggattgga agtttctttg taaattatac tgttgaaacc
     4381 aaaaatttgg gaatcggcga aatgcaagcc ggatttttgc tcggattggg tggtatgggc
     4441 ttgtttgcta ccggccgctt cttaggtagt ttcctgatgc gttatgtgaa acctgaagcg
     4501 ttgctgggaa gttttgcact tatcaatacc ttccttatgc tggtaacagc taaccggcac
     4561 gataatctgg gtatcatcgc attgatttta tcttatttgt ttatgtctat tatgtttcca
     4621 tctatatttg cactgggtat caaaggactt ggacctcaga caaaaaccgc tgcatcggtg
     4681 atggtaatga ctatagtagg gggtgcaatc tgcccttcgc tgatgggctt gataggggaa
     4741 cataatatga atgtcggatt tattataccg atgatttgtt ttgcatatat cgctgttttt
     4801 ggatttgtac agtcaaaagc caggcgttaa cattgaaata ataaatgatt agaaaatgaa
     4861 aaaagtagct ttaataaccg gaataaccgg tcaggatggc tcgtatctgg ccgagttttt
     4921 aattgaaaaa gggtatgaag tgcacggtat tattcgtcgt tcatcctcct ttaatactgg
     4981 ccgcatcgag catctgtatt ttgaagaatg ggtgcgggat atgcataagc agcgtaccat
     5041 taatcttcat tacggcgata tgaccgattc cagttcactg gttcgtatca tacagaccat
     5101 tcaacctgat gagatatata atcttgccgc acaaagtcat gtgaaagtaa gttttgatgt
     5161 tcccgaatat actgctgaaa cagatgcggt ggggacattg cgtttgctgg aggctgtaag
     5221 aattcttggc cttgaaaaga aaacacgcat atatcaggca tctacctccg aattgttcgg
     5281 actggtacag gaagtgcccc aaaaggaaac aacgcctttt tatccgcgca gcccgtatgg
     5341 agttgccaaa ctttacggct tctggattac aaaaaattac cgtgagtctt atggcatgta
     5401 cgcggtgaac ggcatccttt tcaatcacga aagtgaacgt cggggtgaaa cttttgtcac
     5461 ccgcaagatt accctggctg ttgcccgaat tgcacaggga atgcaggaaa agttatatat
     5521 gggtaatctg gattcgttac gcgactgggg atatgccaaa gactatgtgg aatgtatgtg
     5581 gatgatactt cagcatcccg agcccgaaga ttttgtgatt gcaacgggtg aaatgcatac
     5641 cgttcgcgag ttttgtacac tagcatttgc cgaagccgga atagaaatca actgggaagg
     5701 acagggagtg gatgaaaagg ggattgacaa agcgacaggc agagtactgg ttgaagttga
     5761 cccgaaatac ttccgaccgg cagaggtgga gcaactcctg ggtgatccaa ctaaggcaaa
     5821 aacacttctg ggctggaatc cacgaaagac atccttcggg gaactggtca gattaatggt
     5881 agagcacgat atgcgttttg tgagaaaatt gaagctgaga ggggaaatgg aatagaggga
     5941 aataataggc ccctaacccc ttaaacgggg gaataatgag gatttagtca aacataaatg
     6001 caattaaaga gaaaaggaaa tatactaaaa ttccaaatcc aatattaaat aattaactca
     6061 cttaactccc ccttttgggg gtggggggct tacaatggaa aaaacgtcca aaatatacgt
     6121 agccggacat cgtggattgg ttggttcggc catctggaaa aatcttaaaa gcaaaggtta
     6181 taccaatctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aagtcatgtc
     6301 cttccagcta tgataagaaa agtgcatctg gccaaatgtc tgcaggataa caacttcgaa
     6361 gctatccgtg aagatctgaa cagacgacca gtagagggta ttaacgggga aacttcggat
     6421 gataagataa tggcaattct aaaaaaatat ggcattaaaa aaggcattct tcaactatgg
     6481 ggaacaggta cacctttgcg ggagttttta tggagtgagg aaatggcgga tgcaagcgtt
     6541 tttgtcatgg aacatgttga ttttgcccaa ttgagtgagg ggaaaaaaga agtgaggaat
     6601 tgtcatatca atataggaac aggtaaggaa attagcattc gcgaactttc tgcactcatc
     6661 tctcaggttg ttaattatca cggagaaatc cggtttgatg caacaaaacc cgatggtaca
     6721 ccacgcaaac tgaccgatgt ttcaaaactt aattcacttg gttggaaaca taaaattgaa
     6781 atagatgagg gagtgaaaag aatgtatgaa tggtacacag gcaagagttg attttgagtt
     6841 tatcaagttt atcaagtttt taatttaact gcatcttttt tatataaatg gaaataatcg
     6901 atactacact tctggatgaa gttacccgtc aggctaagga gagcccgcgg cttagaatga
     6961 actataatct gcatgatagt ctggaagcaa aagctcagaa acttctcaac gcgttagagc
     7021 agggaaccca attaccgatt catcgtcatc agcatacaag tgagacttat atattgctgc
     7081 ggggaaggtt aaagattatt ttctataacg aacaaaaaga aattgtagat acaaaactgc
     7141 tggatatttc agagggaaaa ttcggaatta atattgctgc aggacaatgg cataccctgg
     7201 aggtactgga gtcgggtacg gtaattttcg aagttaaaga cggaccctat aatccggtta
     7261 atcccgaaga tgttttactt taaaaagtaa tttttaattt ttttcggtta aaattattat
     7321 atttgttgct gtattccgga ttataagaaa caaccatttt tgaatgctta aagtttataa
     7381 acccaagctt aatctaattc tgacagatct aatactaatt tctgtcaata tattgattgt
     7441 tttcaacttt ttcccgttaa caacccaaaa tccaatcagc aaatacttta ttccggttaa
     7501 tttaacttta ctggtatggt tgttggtgtc atacttgttt ggccgctaca gaagtctcaa
     7561 gactgtttct ttcataaaat cagccttttc gctgtttttt acttcacttt ttatactcgt
     7621 cttttttgga ggttatattt ttattcaacc tgccagtccg tattcgcaaa acgtactgat
     7681 taccattgta ttaggtgttt ttgtgagctt gtatttcttt ctgttggtat attatctata
     7741 taaatatgct gttcaatatg acacgttaaa cttcaacccg gataaaaggc tgtcagctat
     7801 accgcatcct tcgcattcgt tgagcgacga tgctgtcgtg gagaggagaa tgcgtattac
     7861 tgaattctcc ggcgaaaagg tattggcctt tcttgaaaag aataccaggt tgtatgagag
     7921 tggtacatgt gtttttaccg aattcagttc aaaagacttc gaaagtgttg aacactatca
     7981 gtattccact tttattcaac taaaacgctt aaaccatata cgaggaataa ataaaatgct
     8041 tgcgatggtc aatgaaaaac tgcctgacga cgggatgttt gtctgttgct acaagtcgca
     8101 gagtaccatt aagcagatga tttatcacaa atataacaat ctgattgcta acagcattta
     8161 tatcatctat tttctatatc accgggtgat gcccaaattt tttctgacaa agcgtctgta
     8221 ctacgatatt actgacggga aaaaaagggt attgtccaaa acagaagtgt tgggcagatt
     8281 gaactattgc ggctttatac tcgaaagaca gttcaaaatt aatgatatga actatgtgat
     8341 agcccggaga gtcaaaaact cggaaccatt actcgacaga aattacggag cactgattaa
     8401 acttaaaaga aaaggaaaag acgggaaaat tttcagcgtt tttaaattcc gaacgatgca
     8461 tccctatgcc gagtttttgc aacaatatat atatgaaaat aatgacctgc aggaaggcgg
     8521 aaaattcaag cgagatatac gcataaccac attgggacga ttcatgagaa aatactggct
     8581 cgacgaactg cccatgctga ttaatctggt aaagggggaa atgaaactgg ttggcgtgag
     8641 accgttaagt actcaatact tcaacctgta ttcgccggaa ttacaggaaa aaagaaccaa
     8701 attcaaaccg ggtctgctgc ctccgtttta tgctgatatc ccaaaaacgc tgaatgaaat
     8761 acaaacatca gaaatgaaat atcttacaga atgcgaaaac aaggggatag tacgtacgga
     8821 tattaagtat ttctttctga ttgtaaaaaa tatcctgata aaaaaagcca gatcagcata
     8881 acttctgcaa tgcagaaaaa taattatgtt aaaaacgtag aacttggtaa aaaataaaaa
     8941 ggtaaaacat ggtaaaaaaa acgattttaa ttctgaatat tctgttcatt gctgtcattt
     9001 cctttgctca gaatatacct caacatattt cttatacccg tatttatgat tttgtagatg
     9061 aactggccaa cgacggcttt tttgaactga attcagcggt aaaaccttat tccaggttgt
     9121 ttatcaccga aaaactactc gaagcaaaac agcaagaagc aaaactgaat gtacgtcaga
     9181 agcgggaact ccattttttt ctggaagatt ataccactga gcaagaccgc ctgcccgaat
     9241 ttgaatttcc gattattgag aatgaaaatt tccgtctgga tttatatcca cccatattta
     9301 attataagga caccctgttt cgcgcccgca ttcagcctat tctggggatg aacatttatt
     9361 ccaataaaca gggacggatt actcagcgct ggtttggcgt ggatttccaa agcatgatag
     9421 gaaaaaatct cagcatttat ggaagtttga gagatatttc cttttcaggc ggacggcttt
     9481 cagaaccttc atatctgaca aactttcccg gttttgagta taaagagccc agcgatttca
     9541 gcgactcgcg gggaggaata acctacggat ggaagtgggg ctccattgga ttgattaagg
     9601 ataatatcgt gtggggagac aactacaacg gttcgaatat catctcgggt agggtgcctt
     9661 cgttcccgat gattaatctc agtgtcaaac ctgcacgctg gtttgaaatg aattacatcc
     9721 acggatggct ggtttccaat gtggtggaca gcagcagtta ctatatggaa aacggagtga
     9781 agaaatggta tcgaaatcac aataaattta tcgctgccaa tatttttaca cttacaccgt
     9841 tccggaattt taatttctca ttcggtaact ccattattta tgccgaaaac aatgtacagc
     9901 ccggttacct gttgccagta gcattttaca agtccatcga ccacaccatg acaaaaggta
     9961 ttgctaccga aaaccagaat tcacaaatgt ttttcaatat cagttcccga aatatccggc
    10021 atctgcatct tttttcatcg gtatttatcg atgagatgaa attcagccgt tttaagcctt
    10081 ccagtgctga gaaaaacccc atttctctca aacttggtgc aaaggtgaat aacttcctga
    10141 ttgataatac aagctttact gcagaatata cccgaacgaa catcatcaac tacaaacact
    10201 ccatcgatgt gctgacctgg gcttcgaacg attataacct gggacactac ctgggtgata
    10261 acgcgcggga aatttacttt tcggcaggat ataaaccgat acggggactg gatatcagcc
    10321 tgagctacct caatgcaaag catggaaatg aatacaatta ccttcgaagg gaaaacaatc
    10381 aggctgtaat caataaaata atcagtcagc cttcacccgg tgatgtcatc tggaaaaatc
    10441 agacgctcgg tttgaaactg gtttatgaac tgttcaacaa cggatatgcg gtaattaacg
    10501 ttgagaacag caatatacag ggttttgaag cgcaatccga accgattacc ggcgaacgca
    10561 gaatgactgc tcaggaagta ctgaattatt acacacctgc ctttttacag ggaaaaaaca
    10621 ctacactaac tgttggattt agttttgggt tctgattatt acagattttt ttcagtgaaa
    10681 tgaaactttc attgcagaaa agacgttata tatgttaaat atcattgttt ttcatgaaaa
    10741 ctccgttaat cattggcatt acaggtggaa tagggagtgg caagtccacg ttctcccggc
    10801 atctgcgtga actcggcctg caggttttcg ataccgatat ggaagcaaaa aatttgcaga
    10861 atttttgtcc ggtagttcgc aataaagtta ttgccgaatt tggtgaggga gtttataacg
    10921 cgaatggcct ggatagggca aaaatggctc agattgtttt cgcaaatgcc gacaagcttc
    10981 agatactgaa caacattgta cacccggaag tgaaaaaaaa attctatgag tgggtaatga
    11041 tacatcctga agagcgattt cttttcatgg aatgtgctat cctgtttgaa ggcggtttca
    11101 acgaacttgt agataaaatt gtgcttgtta ccgcgccaat cgaggagaga attcgtaggg
    11161 tgatgaaccg tgataatgta accgcagagc atgtcatggc cagagttcga aatcaatggg
    11221 ccgacgaaga aaaaaagaga ctggcagact ggacttttga tacgaataat gatattttcc
    11281 cttctgcccg tgtacatgat tttttaagta tgcttgaaca tacgctcttg taaggtcaaa
    11341 atgacttttc attgataagt ttttactcta tattttaagc atccttgcta acaataatct
    11401 cagacaggca tgactgaaaa actgggatca atatgtggtg aacacatccg ggttcttgaa
    11461 cagatggttc cggtagatgt attcgtgaaa ttgcgaacag tcagttttta tagccctgct
    11521 aataattcta atccgccagt catcttttta ggaggtttat caaccgtagt tgaaagtttt
    11581 acctctataa tcagagattt gactaaagat ttcaattttc atgctgtcga gacccgggat
    11641 cacgcatcct ctcagctcgg tgaaaatcaa aaattcgaca tggaaaccat gggaagggat
    11701 ttgggtgaag tggtgaaatt tcttaatctg accaatcatc aatttattct tttagggtac
    11761 tcgctgggtg tggctgtaat agtggaggca ctacggttta tgacgaataa gccactaaaa
    11821 atgatctttc ttgaaccaac caccgtattt cattatcctt catggagtat ccctgtaata
    11881 aaacttagct tactgtttaa aactaaaaga ttgcgtttta ttgcaaaatg gtatatcaat
    11941 aattttgtaa tcgataaaaa agcagatgcc gaaatggctg aaatttcttc gaacgcgata
    12001 gataacgcgg atacacagaa acttaaaagg gcaatacttg caatagccgg ttatgaaata
    12061 tgggaaaaga ttgaacacat tgattgtccg gtactgataa taagtgcttc gaaagataaa
    12121 atgcaccgaa acgatgatat tttgcggttg aactctttga taaccgactg caatataatt
    12181 gatcttgaga ctaataaacg gtcacacagc atggaagctt ctcagataat ccgaaacttt
    12241 attttgacat aaggtagtgt cattgttaaa ccaaagtatc aatattcgga ttatttttac
    12301 cacattactt cactaaagtt acggcaataa gcccaatgat gacttcgtta gcaaggcttt
    12361 taagtttcag agactgaagt tccttgtttt tgtcgagagg gatgttgaga atgactccgg
    12421 cacctccgtc aataacccgt ccgtaaactt ccatagagtc gatattcagc gcattcccga
    12481 tatcggaact tactatgccc gatttaaatg caacacggta tggtcgtggt gtgtttatcc
    12541 tgaaagcctg gccatcggta tagtaatcct gctcaatagt gcaccaggtg tcgggattta
    12601 tcagctgcaa tgtttcactt gttccgtcct tataacttac ggtaaccgtc ccgtttacga
    12661 atcccgactg catgggattg gtagtaccgg ccataagcaa tactgcatgt tgcgctctgc
    12721 cacttagcgt aactgaaagc gaatccgggt aattgtccca taaagtggtg aaagcaatgt
    12781 tgtttttacc atccggaatc cggaatggaa gtcccattgg tgttgtgaag gtttgattac
    12841 gcgttgaccg acgaagtccg gtatcgtcaa tttcgtaggt cagcagcggt ttgcaccatt
    12901 cacctacacc ctgccagggt gtttgcagcg tagtgtaagg cgatcggggc gacaggtatt
    12961 tatttttgaa gatatcggta acggaagcat tcagtacact atcaatattt atctgtataa
    13021 attcagattt ctcttttaag aaatcgggca ggagggtgtt ttttgcaaat gaatggtcct
    13081 gctttcgggt ttcagcttca gtcggctgcc accagctcat ctccctctgc aatagtttta
    13141 caaaaccatc tctcttcccg gttgtttttg ccacagaagg ttttaattgg ttaccttccc
    13201 actcaatttt tatttccggt tgttctgcgt ttccgcaagt gatttctacc aatggaaatt
    13261 caatattttc cagaatagtc cagctggctg ttttaccgtt aattttaact gatttgattt
    13321 tttcgtgagt taccggtagc cgaaggatca gttcggactt tacggcaaat ttgtttttta
    13381 gtaaataata gtcagtcttg ttttttcgcc tgaagttgaa ttcgagatag ggagcagtga
    13441 attcggcaaa attccagtcg cgaggcaaac cggggcgaat gattaccctg ccgttaagca
    13501 aatcgggtga tatgccaaat agtccctgca ctagcgcgcg tgaataaatg ccgaccggat
    13561 cgccaaaatc gcggtaagtt tcgccgcgtg cagcatcgta gaagctgatt tgtcccacat
    13621 ttccgggtga ccggcccagg tacattccgt ccagaatagc gctcttaaac agacgaaaag
    13681 cttcgttggc acgtcctgcc tgccagtatg caagggaggt atgcgcgatt tcagcaaatg
    13741 ctacgttgtt gattgaccag gcgtaaggaa gccagttggt ggtggaaagc acttcgtatg
    13801 ttccattcag cccgtcagct tttaccggga tatgcggaat ttcccgatct acataccggg
    13861 tcgtctgata ctgctgaaat atatcaccaa cttccgaatc gatactgtgg taaacagtcc
    13921 agagggctgc cgaagggtgt gtttttttat agcccataaa atcgacatac tcagcccagc
    13981 atccggtttc tttcagccag agtttatcat tcatagcctt tagaatttta cgggcttcat
    14041 cgcggtaagg tgtcggatct tcgtttatga gttcagcaat acgtgctgtg attttatttg
    14101 cccggtaatt atatgccgac gaatgggtaa ctcctccact gttgtattgc agtgcatcgc
    14161 tcgcccagat gcagcaataa gcatcgtaaa gtccgtcgtc atcagggtca aaattacgtt
    14221 tttcccattc gaggtgacga ttcagcagag gaaacatttt ccgggcataa ttcagatcac
    14281 ctgtccagtt cagatgccac aacagttcat cgacatatac caaattcatg tcgtagtggt
    14341 gcatctggtt actgttgaag ggcgaccggc agatatagcc gttgctgtac atctgtgttc
    14401 cccatttttt ttcagcacgt gccaggtgca aagttgtatc ctgcgacgga tgtgaaacag
    14461 tcggttcaac tgaagtgacc tgcgaggcag cataagcatc gaaatgtttt cttgcgcggt
    14521 cggtatagcc cagaaaatcg ccggtataag cagcccgcca gcctgtaaga ggcattcgcc
    14581 agccaatggc tccgtgcagc catgcgtttt tgccgtccca aactccatcg gcggctgaac
    14641 ttaaagcccc tgctaccgtg ttgatgtatt tatccggtgt tctgatggtt atctgttccg
    14701 agattttttt tgcgcagctt tctgctttca gataaataat ttctccccgt ttgttgtcaa
    14761 tctgctgcaa ttcagctcct tccaacagca aatatgcttt tttatctgta aagttaactt
    14821 ttacatcccc gagcttgttg cctgcttcca gaacatcagg cggatttgta cccatttcgc
    14881 ccaggtgttt gagtttatcg gctttaattt tagatatgac tgatacaaga ttgaagtttt
    14941 tcggaaaatt ttcagtttca attttccata cagccccttc attctcagcc agagcaaggg
    15001 ctgtgatgtg cagtttacct ttactgaata atttatcacg gagttcataa tatcgttttc
    15061 ctgccaaata accagctttg caatattccg cagagtccag ccacagtgat ttattaccgt
    15121 tattgattac aaaacgtata tttctgctgc tatcggcact tttataataa ctggcaaaca
    15181 cagggcgatc gctcgtttca agacgtgatt cggtatttgt tccgtacaat gaacgggtga
    15241 aacggttatt gccattgatg cacacaaaag cattcccttc gggtagatac tgaaatttgc
    15301 gtacaacatc gcgatttatg ggtatgttgt atgaaatacg tgcgagagca tctttgttag
    15361 cggaagtgac aagttgggca ttcagattgc taacggataa aaagcagcaa gccgtaatca
    15421 atgtccggat aatctttgta ttacatttca tttgttatct tttccgtcag ttaaatatgg
    15481 ctcgtcagaa ttatacggat tgtaaactcc ggcagaattc cccttccgga atttacaata
    15541 ttccccttgg aatttattcg ttaataattt tcagttcttt tatttttcgg ggtaaccaga
    15601 ccttcatcgt attaatcccc cgattgctcc atgcgtaata gggtatggcg gtaatgtttt
    15661 gtttacctgt ttgtgccgaa agtccgtcgg gagaaatttt aattactacg gcttcacctt
    15721 ttatttcgtt tattccgttc agtttattcg gattaaaact tactgaaagg ggagcctgat
    15781 caggaaggat aatattatcg acgatgtcgt tgttatcgat cccttcgagg cagtaaacca
    15841 gcggaccgcg ttcgatggcg acctttcccc ggtcatcctg aactttttca ttggcttcca
    15901 cccggtgaac attcatcggc atagtgtatt ccacccgatc accttttttc caggtacgtg
    15961 aaatcacggc ataaccatct tgggttttaa attcaacttt cttaccgtta atttttattt
    16021 caatttcttc agctccctct gatacatatc ggtacaaatc gcccggaacg ggttggttgt
    16081 gtgcccagcc gggtattcga agatacagtg aaaagactga tttcgaggca gggcttacct
    16141 gcagggcgat atttccgttc cacggataat ccgattgctg gctgatctca acactggttt
    16201 tcggaagttt gaaacgtgtt ttactgccga taaaaagatt tacatatatc ttgtctgttt
    16261 tttgcgcata aatatatccc ggaacggatg caatgaaccg gcataagttg gttggacaac
    16321 aagaacaatc gaaccatggc tcacgggtga gtttgttgcc ggagttaaaa gcgtaacggg
    16381 cattacattc aagcggattg ggataaaaaa atgttttgcc atccagtcca actccggaaa
    16441 taacgttgtt gtacaggctt cgttcaagta catcgatgta ttttgcatct ccgtggagca
    16501 aaaacattct gtagttccag tacacattgg caatagcagc gcaggtttca ttataagcag
    16561 tcagattggg aagttcgtag tctgcaccaa aggcttcgcc tgcatgcagg gcaccaatac
    16621 caccggtgat ataaaatttt tttgtaacgg cgttctccca tattttgtca atagcttcga
    16681 tatattcttt gtttccggtg agcgcagcca catcggcagc ccctgaatac agataaccag
    16741 cacgaacagc atgtcctgtg gcctcggttt gttttacaaa gggaagatgg ttttgcgagt
    16801 aattttgttt tgaattttta ccacggcaat caataaagaa ttttgccaga tcgaggtatt
    16861 ttttctcacc cgttatcctg tacaaccgta ccagtcccat ttccacaatt tcgtgtccgg
    16921 gggcgtcatc ccgttttccg ggaccgaata cctgtaccag cagattggca tttttcaggg
    16981 cgatattcaa cagattacgt ttgcctgtgg cagtaaaatg tgcagcagct gattcaaaaa
    17041 gatgcccgga attatacaac tcgtggctga gttccgattc tttcacccac cgttcactac
    17101 ccgcccagct atgtgtattc ttaggatcga tggtgcgggc agtgtaaaga taaccatcgg
    17161 gttcctgcgc ggctgcaact atacttatca gactgtccac gtaatgatcc atcgctttgt
    17221 cgggatgtac ggacatggaa aaagaagcgc cttcgatggt tttgtagatg tcggtgtcat
    17281 cgaacgggta aacggtcaaa aattttcctt ttttggcacc tgatgcaaga atgaaatttt
    17341 ccaccctgcc ggtttcctca catttccgaa aggaagacgg gatggtgact gttctgttta
    17401 tttctatttt aggcaaccag aaatggtcat tcaaacttac ggtattaaac ggaataccct
    17461 gaatgttgta atccacaacg ggttgcgatg agaaagtcag ctgcgatgtg taaatgaatg
    17521 caacagtcag catcattttc agggaaattc ctgattggtg ttttctgaag gatgagaaaa
    17581 taaatgtttt agtcatggga taaatatatt tcaggttaat ctacttaaat aaaatgcttt
    17641 atgctgtagt catgttgctt gataaatttt gttggcatta attgaaactg aaaatagatt
    17701 tcagaaaatg gttacatatt gactatttta caaaatcaga aaaattggta caaataaaca
    17761 acttttactc tgatttttgg taatacaaat gatatttcag gtgacattta tgatttgaca
    17821 ccagtaattt tacaattttc ttttttttct ttattttttt gaaaagataa cgaactgact
    17881 ttaaatgaaa attccggatt acagcccgtt tactgtaatc cggaatatgt tcccgttttt
    17941 agtgttagtt tttctttttc agtacgtacg cgtctgcaag ttcaccttcg gtcacttttc
    18001 cttcggagtt gagcagcgta agggtaccgt cacccagcag gtacgattgt tcgctgtcag
    18061 ctgatttcag tacaaggcgc gacccgggtt caatcagcgt aaaagtacca gtttcttcaa
    18121 aactgttatc cttacccaga taggttgact tctgaatata ggtgctgtct tcctgaagtg
    18181 taattacggt ttcaattcct tcgcagtcgc cgcaggggaa aaagccggca tattgaccca
    18241 accagctgtc ggattgaaca tcggcacggt tttctgtaat aacagtcacc gagtctttta
    18301 caggctgttc gctggtattt tcactctttt gaacacaggc ggtgatgcct gccattatca
    18361 tcagcaatat taaacttttt ttcatcatca gtgatttcat ctgttagtta tagttaggta
    18421 atttagaata ctccctggaa taacgcagat tctgatcaac gtaattttct gtgtacgaca
    18481 cagtaacatc ttttattttt ccttttttat cggtaacaag ggtgtaaacc gggttgacaa
    18541 aacctttgta aggtgccaga ttgagttttt cgtaacgtgc cagaacttct ttgtgcagat
    18601 ctgaattaac tttaacagca tatttttcca ccaagtcacg gccaccgttg aaatcaccgg
    18661 ttgatttgat gcgctgtatt tcggccagca gtttcccgaa taatatccgt actttgttgt
    18721 aatcattcac cacaacgaag gatttaccgt cacgcaccct gaattctacc accttttcgg
    18781 ctttaccgtt ttcatatacc cagttggcta tcagctgccg gttgcgcatg tgtgcttctt
    18841 caatgttttt tcccggtttg atgcgggtga gctgagtcat cattccgttc atcaggaact
    18901 gataatactc ggctttgaaa gcttcgttgt ttggtaacag tcccaattca acaagtttag
    18961 gatcgcctat gtagtaaagg ccaaataaat cggcacgtgc ttcctcgatg gtggagtcgt
    19021 aagctttcag cgcgttggga tcagtgccgg gcaggagttt gcctgagccg tgaccgagac
    19081 attcatgcaa atcagtgtgc aggttgttag tcagcgatgc atattctttt gcgcggttac
    19141 gttcctcgtc cgaccacatg aattcttcgt tgaaaccatt gcccatagca acctgatcat
    19201 atgcttcggt aatattttca atagttacag atttagaacc atactctgcg cgtatccagt
    19261 tggcgttcgg caggttgata ccaattggag tgggagggta acagtcccct ccgagcatgg
    19321 caaccgtgat tactttagct gaaacacctt taaccttttc tttcttgaaa ttcttatcga
    19381 ccggcgaatt gtcctcaaac cactgagcat tgctgctgat aatttcggtg cgtttggtag
    19441 cttcaatgtt tttgaagtta actatggact cccagctggc tttcaatccc agcggatcgg
    19501 cataggtttc ggtaaaacca ttgacaaaat ctacctgaga agtcagatct tgtacccatt
    19561 tgatgcaata ttcatcgaat gttttaagat cacctgtctg gttaaagcga atgagtgtat
    19621 tgatgatatc ccgttgctta tcattttcag ccacttcagc tgctttttcg agccaaccca
    19681 ctacacggct cagtgctttg gagtacatac cgccaatttt gtaggttttt tccacaattt
    19741 tcccgttttc tttcatcagt ttgctgttca gaccatagga tatgggcgtg ctgtccctcg
    19801 gatttttcat ttttgtataa aaatcttcgg cttcctgctg ggtaactcct tcgtagtagt
    19861 tattggccga agttaggatc aaatccacgc catcggcctg attggtacgt ttgggataaa
    19921 cagtgggatc gaaaataacc ggactaatcc gggcaattaa ctgatcaacc gtttcaccct
    19981 gctgcaacgg gagttttgca gggtcaatac tttttactgt ttcagcaaaa taggacgtgc
    20041 tgaatgcggg tgtgaattta tcttcggaat aatgatgatg gataccgttg gccatccaca
    20101 cacgcttcag gtaggtagta agctgtttga aaggttcggc gtcgcggtca cccttgtaat
    20161 tttcatacac tgcttcgagt atgcggcgaa tgctcagatt atatttgttg ttctgatcgt
    20221 acaggatatc gcgcccttcg atggcagcct gcgacagata gtaaatcatt tctttctgtt
    20281 taagcgatag tttttcgaag cccggaacgc ggtagcgcag aatttcgaca tctgcaaatt
    20341 tatcgaccga gtagttgaaa tcttctaccg aaggtgtggc attttctttt tgtttgttgc
    20401 ctccacaggc aatcagggat gaaatcataa ggaataaaat taattttttc attggaaatt
    20461 aagtttacat cgtaatattt agcattaact gatttgtaaa acacaaagtt acaaaaaaaa
    20521 gttgaacaga gcgtgtgagt tgctgaaaag taaggactgt tgatatgtct ttagttttat
    20581 caacatatac cggtcttttt tttaataaat cagaatttcc tttttgcctg catgtggagt
    20641 ttgattacct ttgtaactat gttacaaaat tttatcagcc agcgcatccg tcacgaatta
    20701 ccttttgaac caaccgaaca acagtcgttg ctgattgaag gtttgggtga atttgtcatg
    20761 tcagctgatc ctgaaaaact gtttctgctg aagggttacg ccggaacagg aaaaacctcg
    20821 gtggtaagtg cgctggtgcg cgcactgaac agtctggaac aaaaaaccgt gttgctggct
    20881 ccgacgggac gcgcggcaaa ggtgctttcg ggctactcgg gatttcctgc attcacagtt
    20941 cataaaaaga tttatcggca gaaatcaatg tccgaattcc gttttcagtt ggctgataat
    21001 ctccataaac atactctctt tattgtcgat gaggcttcca tgatttccaa cagtggaata
    21061 gatggctcat tcgggagcgg acgcttgctg gacgatctga ttgaatacgt gtatagcggc
    21121 gacgagtgca gcctcctgct gctgggcgat actgctcagc ttccgcccgt tgagcaaccg
    21181 caaagtccgg cgctcgaaac cggcactctg acagcatacg gactgaaagt ttacgaattt
    21241 acacttaccc acgtggtgcg tcaggcgctc gaaagcggca ttttatacca cgccacacaa
    21301 ttacggctag atttggtcga aaaggaagag tttagtcatc cgaaattcga acttcaaggt
    21361 tttgcagata ttaaaagact taacggcgtt gaactgattg atgaaattca gcgggcatat
    21421 gatggagtgg gtgtggaaga taccatagtg attacccgct cgaataaacg ggctaatatg
    21481 tataacaacg gcatacggtc gcgggtgatg atgaaggaag atgaactttc aaacggcgat
    21541 ttgctgatgg ttacccgaaa taattatttc tggaacaagc catatcagga tattgaattc
    21601 gtagccaacg gcgatgtgct ggaaatcatc cgcatcggaa aataccgcga aatatacggg
    21661 tttcacttcg tggatttaag cctgcggtcg ctcgattacg gctgggaaat tgatgcccgt
    21721 atctggcttg aaagtctgca atccgaaaat ccagctgcca tgaatgaact gaccaataaa
    21781 ctctttgccg ccgtagccga ggactaccct gaattaacca accggaaaat gctgtttaaa
    21841 aaaattctgg aaaatgaatt cttcaatgcc cttcaggtga aatttgccta cgctgtaacc
    21901 tgtcacaaag cacagggtgg acaatggaaa actgtgttta tcgatcaggg ttatttgccc
    21961 gaagaacaga tcaaccgcga gtatttccgg tggttctaca cggcgcttac ccgtgccacc
    22021 ggtcaggtat ttctggtcaa tttccccgac gagtggtttt aattaagatt gaaaatctaa
    22081 caaccgacac ttcattcttg aaattaatgt agaaattttc tttaacaatc cgaaatcttt
    22141 tgtattttcg cagacaattt tcttttattt aatttaaaat tatgagcaaa aaatctgttt
    22201 ttattacctt tcttgcactg cttgcagttc ttacctcgtg taaaaccaaa gatccggcat
    22261 ccgatgaagt gaatatagtt tcacaatttg tatatgacgg gttatcgacc tattatctat
    22321 gggctgacga aatgaagaat aataaaccca cggtggccga ttccgacccg gaagtatatt
    22381 ttgaaagcgt actcaatagt ctggataaag aaaacgggtg gtcctttatt actgacgatg
    22441 tacaaggtct actgtcagat ttcagtggtg agccaaaatc ttttggtttc tcgcttacct
    22501 ttgcagccgc caacgatgcc agaacagaat attatgcagt ggtcaaatat gtatttccca
    22561 atactcctgc agccagagca ggcattggac gaaagggaat tattactaaa attaacggac
    22621 aaccaatcac cgaaaataat tacatgctgc tgtatggcaa cagtacgaca gaattaaccc
    22681 tgtataaaat tgccgatggt cagctgaagt tcgatcatat tgctaatatc actcccgaaa
    22741 ccattcagac aaatcctgta tattattcaa atgtttatta tagtggtggc aagcggattg
    22801 gatatctgtt ttacaccgat tttatcagta attacaatga tagtctgtac aacactttca
    22861 gccgttttaa aaatgccgga gtgactgacc ttattctcga cttgcgttat aatcatggag
    22921 gtgcagttac tgcagccaac tacctctgtt cgctgatcgc accggcaaca gtcgtggaaa
    22981 aaaaatcgcc actggtaatt atgtcataca acaactttgt gaaccagtat ttcgataagg
    23041 aaaaatggag ccgcaccgat aatctggggg cgtacgatga aaaagagaaa gatccaatgg
    23101 gagcaaatct gaatttgaat aaagtttata tcatcgcaac cggcgattca tattcggcat
    23161 ccgaactgac aactttctgc ctgaaacctt atatggatgt ggtacatata ggcgagaata
    23221 cgggaggaaa atttacagct tcgtggacca ttcatcctta cgacagcaat atcggtctga
    23281 atatttacag tgaatcttcg ctgagtgcta ccgaaaaaac taaactggcc aactgggcta
    23341 tgcagcctat tgtagccaaa tataccaata aaaatggcgc agatttttca aacccgggtt
    23401 atctgatacc cgattatcca ttggaagagg gatttggcta tatcagcaac tgggctgcca
    23461 ttggcgatcc tgctgatgtg tatctgggtc aggcacttta cctcatcacg ggtgaagaaa
    23521 gctataaacc ggtacaaaaa atcggaatga gtcgtgtgaa agcaatgcgc gaaacacctg
    23581 tgaaacttca aaatccacgc gatgtgcgca gggaagccgt tatactggat aatgtgaaat
    23641 taaaatcgct gccacacacg caaatgcaga atatcgtgcg gtaatcaggc agttaaaaaa
    23701 tacaacggga gggaatgtga aaacatcacc tcccgttttt ttattcagtc gatcgaaggg
    23761 gcagtttgcg gattttatcc ttatcaagtg cgtcgtattt cagatactgt tcacgggtaa
    23821 gaattttttg taattcgata tctctttgtc tgttatatat atccgtccgt ttttgtatcc
    23881 tgcaacggca tcctgtctct gctttctgca tattgagcag gtattggtac tcgataattt
    23941 cgagttgtct ggcctgaaca tcgtcaaagt ggataaattt ctgcatctca gtcaccttcc
    24001 cactggctgt gcgactcagg atttcccgga ttacagattg ctgctgtgtt ccggcaatgg
    24061 tgaaaccgga aagaagtatg attgttgaaa gtattatttt ttttatcatt gttgcatttt
    24121 ttttgttgtt cattcccggt ggttattttc attctaaatt tgttacatca aaattcctgc
    24181 caatccggtt attcgttaca caagtcaagg tcatcagaat tctgaattga ttctgaattc
    24241 acaaaactac gattgaatat tcgagtcggc aaattctctc aacatcacca atgcttttcc
    24301 agaagttagt acatgcgatg aaaaaaaagt tccggcgaat attcaccaga actttttaac
    24361 aacaacttca caataaaatg tgaaatatac gtaaacaaac attattgact taatatcagt
    24421 aaaagtccag ttcgtcttac acaacactta tagttcgaac tgtttacaca ccaaaggtat
    24481 tataacttag aatgaaatca aaacatttgt ttttcgaatt gtacaattca aaactataag
    24541 ttgcagaata tgaaattttt agaattttct tctgtcggac tgcttattga gattgctgcg
    24601 gaattgcgcg ggagtcattc ctgtttgttt acgaaatgca tcaataaaca cggagttgga
    24661 attgaaaccg gcaatattac cgataacagc cagattgaat ttgtttgagc ggttgtgagg
    24721 atctgaaaaa atggcaataa cttcctctac ccggtattta tttatcaggt cgttgaaccg
    24781 gatgtcttgt tcgctgatgg cacgcgagag gtattcacgg ttggtgttaa gtcctttggc
    24841 tacggtatcg agcgtaagat ctttacggct gaaattttta tctgtttcaa gccagagcgt
    24901 cagcagttcg aagagttgaa ggtttttgtc attattctcc gatacttttc ttgtattttt
    24961 tcgtttgtta tcttccatct gcaattgcct caatctgcgt tcatcctctg ccagttgtat
    25021 gtttttctga ataagtgaaa tcagcacatt gttttttttg tgtagctgtc tgtataagaa
    25081 agatgttatt attcccgtaa ttacaagaaa aataatggta atgaaggcga tggtcagttg
    25141 tttgtgttcg gtagctcttg atttctgaag attaaaggtc gtggtagtga gttctatatt
    25201 ggtattgtac aaatctttta tactttttat aagtgaattg gaggtgctgt cctgatatag
    25261 cttggctgtt tcaagtgctt tatatgccga ctgtgaatta tttacagccc tataagcatt
    25321 ggatagttta taataggtct gctgtttcag ttcaagataa ccgctgtgag gcaaagactg
    25381 actggcaatt aataaatgtt gaagcgcctt ttgaggcata ttcatagtga ggtaaatatc
    25441 cgataacttt acatgtgcat aagtctggcg gattgtggca gtccggtaat ttgaagggag
    25501 ttgaagggtt ttattcagat attccagagc ctcatcgtat ttgttcaatt tcatatcaac
    25561 ttcggctatg ttgttgtatg ccagtgaata aaatgagttt tttataatac ggttactcag
    25621 gtcaaggcat ttgtaggcat aataacgtgc ttcctttggt tgatttgtca ggagacagta
    25681 atcaataaaa ccgatatata tgtaaaaggt ctcttctttg tcattgtgtc tggaaataat
    25741 tctttcagca gtttttaatg cctgcagagc tttggcgtag ctgccgttat tcatgtaaaa
    25801 tctgcccagc gatttcagat tgattgcttt tcctttttcg agtcctgcct gctcaaaatg
    25861 ttgggtggag agagttagcc attcaatacc tttatgctga tcgctaagta caaaagcaat
    25921 ccgggcaagt gttcgctcgt tcagtgccaa tgccctgata tccttgtacc tcgaattcgt
    25981 ggcgtttaac agcgcctcat aggcattgac aaggttatcc tgtctgaata atatcagtcc
    26041 actgatgctg ttgtaccaaa acatgaaccg atcgttgtca ggtatgggtt gaatacttct
    26101 gaacagtaca gatgcactat ctgttttttc gctttcacag agtgccgctg ctctgaaaag
    26161 cgaagccatt cttttaattt catctgttga ttgccggcta tttaatttat caatttctgc
    26221 tattactttt tcattctgat tttcctggta gaaaagacta atagcattta ccaacggttc
    26281 gggatatggt gtgacagtcg atttactgca tgcgtaacat aaaaacacgg atatacatcc
    26341 tgctaatata taaagatttt ttttcataaa ggctcctttc cctgtattat tatgctgtaa
    26401 accgaacgat aactgtcatc agtttacagc ataattttct cttctttaag ttgtctgtaa
    26461 aaatataaaa aatccgaaat ataaatgaac tttttactga aaacagataa ttataat
//
LOCUS       DAOPYB010000023        26299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1833_length_26299_cov_10.873419, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000023 DAOPYB010000000
VERSION     DAOPYB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26299
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1833_length_26299_cov_10.873419"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1260
                     /locus_tag="PKH58_03280"
     CDS             145..1260
                     /locus_tag="PKH58_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HNX88082.1"
                     /translation="MNYSKRYSELNEQFIPGKNNRLSDTNIDIRFIPFVSSVVNSDDA
                     AWSFNPADREIAENISFEYPVFIPRGKEKYEHAILLMHGLNERSWNKYLTWAEYLCIT
                     TGKPVILFPIAFHMNRSPQAWTNPRCMQHALTSRRKLYGEDRSLSFANIALSERISKN
                     PLLFYSSGRQTVFDLEQLYREIKQGIHPLFSSGARIDIFGYSIGAFLSQIVLMSNPSG
                     LFSDTRLFMFCGGGIFSSMYGVSRSIMDRAAFDHLYQYYLNDFGLTQKLKPDKVFDAF
                     YSMISPDRNRSEREAFFAQMGNRLCGIALEQDLVMPYVGLQQALGTDCSNEKINLQDF
                     PFSYTHENPFPIGAHTNQIAVNDEFNNVFSQAAGFLT"
     gene            1440..2357
                     /locus_tag="PKH58_03285"
     CDS             1440..2357
                     /locus_tag="PKH58_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HNX88083.1"
                     /translation="MKKLLLFIPCIMFCLSAIAANFTVDGIYYTISGSNVSVTSGAYT
                     GAITIPATVTYNSITYPVTAIGNMAFASCSDLTSVSIPSSVTAIGDDAFYRCTGLTSL
                     VIPTSVSSIGNYAFEYAGLTSISIPSSVGTIGQHAFNFCTSLKTVTIPASVSFIGIEA
                     FWACSALTSIYVYRSVPLDLTSSDNVFYGVNKTSCTLYVPVGTKTAYQAANQWKDFVN
                     IVEFTTAVPRVDDERINIHPNPVVDNFKIDGLEGTIDLILMDINGKELLHKEILKDEN
                     VSVSEFPAGMYFVKLSNNGLNYKKKIIKK"
     gene            2717..3847
                     /gene="hisB"
                     /locus_tag="PKH58_03290"
     CDS             2717..3847
                     /gene="hisB"
                     /locus_tag="PKH58_03290"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HNX88084.1"
                     /translation="MTILSKKVLFIDRDGTIITEPPVTYQVDNLEQLEFLPAVIRNLY
                     FIRHKLDFEMALVTNQDGLGTDVYPQVNFDTVQRKFMQTLSNEGVWFDKIFIDKSFPE
                     DNLPTRKPGTAMLKGYFTDEYDIAGSYVIGDRATDVELARNLGCKAIYLSNDTEELQA
                     KGLTEFCALQTTDWNHITEFLFAGERAAVIKRTTKETDIEVEINLDGSGKSEISTGLR
                     FFDHMLEQIGKHSGADLKIEVKGDLEVDEHHTIEDTAIALGEALLKALGDKRGIERYG
                     FTLPMDDCLCSTALDFGGRAWLVFDADFKREYVGDFPTEMLLHFFKSLSDAARMNLNI
                     YAKGENEHHKMEGIFKSFAKSIKMAVRRDIYNYELPSTKGVL"
     gene            3881..5464
                     /locus_tag="PKH58_03295"
     CDS             3881..5464
                     /locus_tag="PKH58_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNX88085.1"
                     /translation="MRKFLILLFSFLCIVSQAQIKTDEPPRLLIGIKVDGMQNSHIQK
                     LWKYFSAGGFRKIITEGTVVEQMEHPVVSAGNSADVATIFTGTVPFYHGITGDYYFDR
                     EDKEIKSILSDENQVGIGTREKFSAHQMLSSTLTDELLLATPQSMVYSIAINPEDAIM
                     MGGHMATGVTWMDDSMNKWVTTAYYTKGLSRWADLMNVNGSFNSIASTDWVPFSSISS
                     YLNPTVKGSKSVPFKYNPLEKGKNPARSILRNTPAANNLVTELAQTIFDKEALGADKY
                     TDALLIQYTVKVPGQLTSSLTNAEQEDMYLRLDRDLQNLVSSISSRVGSDKVVFFLTG
                     GTEDLHSPVELGNNRIPAGYFNANRSLSLVNTYLMALYGQEKWISGYYGKNIFLNKKR
                     IEEKKLNFEEVKSRTAEFLLEFEGVQAAYPINDIVNFTGNSVDVRSKYRNSYHKTTAG
                     DIVITLLPGWVEIDDKNQVVGTANTPRVYLPFYLYGARVKPQKISTDCKTTDIAPTLA
                     YLLGVPLPNACIGKSLIPD"
     gene            5624..6796
                     /locus_tag="PKH58_03300"
     CDS             5624..6796
                     /locus_tag="PKH58_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNX88086.1"
                     /translation="MNYNFDEIIDRRNTGAVKIELCKKMFGTEDLIPLWVADMDFRTP
                     DFIIDAINERCKHPILGYSVPDDEYFDSIIRWIETRHGWKLEHNWLGFLPGIVPGLAF
                     AVNAFTRVDDEIIIQPPVYPPFINVPSKNGRQLVFNPLKEVDGRFEMDFEDLERKITG
                     KTRLFILCNPHNPGGRAWDAETLTKLAAICHKHGILVVSDEIHSDMALPGNVHTPFAT
                     VSELAEQNSITYMAPSKTFNMAGLVSSSYIIPNRDIRKKFSDFLENSELANGNIFAYI
                     AAKAAYENGTEWLNEMVDYIQGNVEFVVDFLRNNIPQIKPMVPQASFLIWLDCSGMGM
                     NTNQIQNFIINEAGLGLNKGTTFGPGGEYHLRLNIGCPRAILVEAMQRLKQAIDSK"
     gene            6828..9233
                     /locus_tag="PKH58_03305"
     CDS             6828..9233
                     /locus_tag="PKH58_03305"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNX88087.1"
                     /translation="MKNILYIFLALLCITETLAQTRKSVEAYLISSSMTIDGKLNEPV
                     YTQALPAKDFVQLQPYNGKPSVKPTEVYFFYDQTAIYVGAKLYDDPDSIFNYLTERDN
                     TGMSDYFGVYIDPYNQGQLAFGFFITPAGVQVDVKATKSDEDIEDPNWNAVWESKTQI
                     NADGWTVEMKIPYSAIRFSKNANNSWGLNMFRNIKRHNSNNSWNFIDRKVSGFIHQEG
                     ELIGISNIKPPVRLSLSPYLATYFQPKSKDASAGFLYKGGLDLKYGLNDAFTLDMMLI
                     PDFGQIQSDDRQLNLSPYELYYDEKRQFFTEGTELFQRGGIFYSRRIGAAPKFSASGH
                     LNDGEVVDYNPAETQLVNATKVSGRTQNGWGIGVLNAMSLPSEATLKNAETGSTREVQ
                     VQPFTNYNVAVVDKSLKNNSYISLINTNVLMAGNPFHANVTATEFQFRDKTKTYALKG
                     KAGVSVRGNETIETGFYSTLRFEKNKGNWLYGAAENLYSDKFNPNDLGYLERNNLSNT
                     EAWFYYQVIEPKGILLEWNFGLWSNYNRIYNPNTIFDTEAGYEASARFKNNFFLYSNA
                     NVASRQNNYYEPRVKGHYYLNPVNYKFNIQLTSDTRKHLFFQGGYGYRVQPEIGSVEH
                     AAELESYLRIGQRFVFDYSIGGNKSNNERGYVMNNAAGDSITFARRDVSTLINTLSAS
                     YIFNYKTSLTFRMRHYWSGVSNKEFFLLNDDGTLNAYPDFSQNMDQNYNAFTIDMIFR
                     WIFAPGSELSLAWKTNSYDNHNEVIYNYQDNLIKSWLNQKNSISLKVLYYIDYNRLRK
                     KEI"
     gene            9258..9920
                     /gene="ung"
                     /locus_tag="PKH58_03310"
     CDS             9258..9920
                     /gene="ung"
                     /locus_tag="PKH58_03310"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNX88088.1"
                     /translation="MEVKIEESWKQRLSPEFEKPYFIALTEFVHAEYRAKQIFPPAKL
                     IFNAFDSCPFDKVEVVIIGQDPYHEPGQAHGLCFSVNDGIDIPPSLVNIFKEIERSLG
                     KTIPTSGNLTRWAQQGVLLLNATLTVQNHKAGSHQGKGWETFTDAAIRTLAAERENLV
                     FLLWGNYAQQKGAFIDPSKHLVLKSVHPSPLSAYRGFIGNNHFVLANNYLKEYGKTEI
                     DW"
     gene            10124..10987
                     /locus_tag="PKH58_03315"
     CDS             10124..10987
                     /locus_tag="PKH58_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNX88089.1"
                     /translation="MKWINRNDDESNYEDRRGRSTGRRLAAGGGIGTIIILALYMFTG
                     QDFSSVADMFTGGEAQTETVDPSRAKENEDLKVFSLGVFNSANDVWTKIFNEQMQMEY
                     RKPTFVTFTDQTMSECGGASGATGPFYCPGDEKVYIDLSFFHELATRFKAPGDLAMAY
                     VTAHEVGHHVQKLLGITDKMQQYRGRISEEEYNQLSVRLELQADFYAGLWARYAEAMN
                     IIKLEEGDIESALGAANAVGDDTIQKQAQGYTVPDSFTHGTSAQRMRWFMKGYQTGDY
                     RQGDTFNAETL"
     gene            complement(11206..11985)
                     /locus_tag="PKH58_03320"
     CDS             complement(11206..11985)
                     /locus_tag="PKH58_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX88090.1"
                     /translation="MVEKLFTNTTAEADELIRLSRETGKILTAYHNRRFVSDTKTVRK
                     ILNSGILGDLVDYETHFDRFRPQPRPTGYWRENPLPGSGKFYDLGSHLIDQALHFFGL
                     PQAVTAHISTQREWAQTDDCFDVKLYYPTHTATLKSSMIARIPGPTYQIHGTKGSFVK
                     YGLDVQEENLNKGMIPNTPDWGKEPESIRGKLVTDIEGVMMEAYVESEKGDYREYFIN
                     LRDAIHGFAQLAVKPEEARNVIRIIELAFKSNREKRTVEFE"
     gene            complement(12083..12634)
                     /locus_tag="PKH58_03325"
     CDS             complement(12083..12634)
                     /locus_tag="PKH58_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNX88091.1"
                     /translation="MNLTSRQRAEFLDVLKKRFEKNLQRHPNIEWEKIHEKLEANAEK
                     LFSLHQMETTGGEPDVTGHDAATGEYIFVDCSAETPKGRRSLCYDREALESRKEFKPK
                     NNAVDMAAEIGIELLSEDEYRELQKMGEFDLKTSSWVKTPAEIRSMGGAIFCDRRYNH
                     VFTYHNGAESYYAVRGFRGSLRV"
     gene            complement(13025..14083)
                     /locus_tag="PKH58_03330"
     CDS             complement(13025..14083)
                     /locus_tag="PKH58_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HNX88092.1"
                     /translation="MELQSILLPGVDPKRPLIISGPCSAETEEQVMATAKALSNIGVK
                     IFRAGVWKPRTKPGGFEGVGAEALTWLKRVKSELGMYTAVEVANARHVREALANDVDI
                     LWIGARTTVNPFSVQEIADALEGTDIPVLIKNPVNPDLDLWIGAIERIYNAGIRRIGA
                     IHRGFSSSDKKIYRNIPQWHIPIELHRQLPTLPIICDPSHIGGKREMIAPISQQAMDL
                     NFDGLIIEAHCTPDKAWSDAAQQITPEILQLILDSLVIRETNQTTENLTVLRRQIDSL
                     DNDLLELLAKRMRVSKEIGTYKKEHNMPILQAQRYDEILKNRVNQAEKMGMNGDFMKT
                     VLMAIHEESVRQQMLIMK"
     gene            complement(14217..15428)
                     /locus_tag="PKH58_03335"
     CDS             complement(14217..15428)
                     /locus_tag="PKH58_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX88093.1"
                     /translation="MSQTSNNASLLPFEGAGGQELIQPANRLNSVSEYYFSKKLREID
                     EMRAQGKDVLNLGIGNPDLPPSEETIDALCTEARKPNVHGYQSYTGIPELRNGLAAWY
                     KRWYNTELNPKTEIQPLIGSKEGILHITLAFVNPGDGVLVPNPGYPTYSSASRICEAN
                     IISYDLDENNNWEPDFDALEKTDLSNVKLMWVNYPNMPTGANASVELFEKLVAFGKRH
                     SIVICNDNPYSFILNDRKQSILSIPGAKDICIELNSMSKSHNMAGWRMAMLASNEQFV
                     KWVLNVKSNVDSGMYRPMMVAAVQALQNTDEWHDKMNEVYARRRELAYRIMDTLECTC
                     DKNQVGMFVWGKIADKWQDAGEIADKILYEKNVFITPGFIFGDKGKRYLRISLCSPES
                     MLEEVIRRITI"
     gene            complement(15531..16379)
                     /locus_tag="PKH58_03340"
     CDS             complement(15531..16379)
                     /locus_tag="PKH58_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNX88094.1"
                     /translation="MTKRIAIQGVSGAFHEIAARGYFEDEDVEIVCCATFKDVFKAID
                     DDESLLGIIAIENTIAGSLLQNHNLLRESGCLIIGEYKLRISHQLAALPGQEMDDIHE
                     VYSHPIALMQCEDFLDAHKNLRAVESDDTALSAKEIAENKISGRAAICSRLAAELYGL
                     EIIAEGIETNKRNFTRFLIIAKPDLAYKMNRQIALNKSTLVFTLPHEEGSLSKVLSIL
                     SFYHMNLTKIQSLPILGREWEYQFYINLTFDDYERYRQSLDAIKPLTKEFQILGEYRE
                     GKTPED"
     gene            16879..18174
                     /locus_tag="PKH58_03345"
     CDS             16879..18174
                     /locus_tag="PKH58_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX88095.1"
                     /translation="MTNKIEKKSYALPIAMMFALFFMISFVTGLPSPMGVIVAKQFGA
                     SNFESQLGFLANFIAYAFMGIPAGLMLKKIGYKKLALVAVTVGFTGVGIQWLSGQVGS
                     FTVYLIGAFVAGFSMCMLNTVVNPMLNTLGGGGNKGNQLIQWGGTINSSAATIVPILV
                     GYLMGDVAGRTIDKASPALFIAMGIFALAFIVLFIVDIPEPHIQKVSEKKVKDTHSPL
                     SFRHFILGAIAIFIYVGIEVGIPSTANLFMTALPSAANPGLGIDAGTAGIVVGIYWLL
                     MLVGRLVGASVGAKISSKTMLTFASSVAMVFILLAIFMPVSTTINMPVITNTFSFAQV
                     QVPVNVLFIILCGLCTSIMWGGIFNLAVEGLGKYTAAASGLFMVMVCGGGLIPVLQGF
                     VSDNFGFLSSYWVMFLCLAYLLFYGVIGSKNVNKDIPVE"
     gene            18194..19159
                     /locus_tag="PKH58_03350"
     CDS             18194..19159
                     /locus_tag="PKH58_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNX88096.1"
                     /translation="MTMEMEKPYVIGIDMGGTNTVFGIVDARGNVIAKGAIKTDNPDI
                     NSYISDLKTELDKQIEVVGGIEKIKGIGAGCPNGNFYTGNIEFAPNLPWKGVVPFAKL
                     LQDTFGVPAAITNDANAAAVGEMTYGIARGMKNFIMITLGTGVGSGIVIDGKIVYGHD
                     GFAGELGHVRVVRNNGRVCGCGREGCLETYTSATGVARTAREILEMCDTPSVLRSIEL
                     KSITSKDVYDAAMEGDEIAKEIFEYTGKILGEALADFIAFSAPEAIVLFGGLAKSGDL
                     ILKPVVDNMEKNLLSIWKGKVKVLFSALKEADAAVLGASALAWEL"
     gene            complement(19214..19792)
                     /locus_tag="PKH58_03355"
     CDS             complement(19214..19792)
                     /locus_tag="PKH58_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88097.1"
                     /translation="MKIHVMSLLFLLITMHVFSQNNKSNLYSGGMLFYQPGYTTTSTD
                     YQTLKNNSNSIGGILRFYISDYFTAGIYGGSQRTKYITEGSDNSYISLGYGGPFAGVS
                     HKSGKFRYTLSGFAGMGSVKNLHVQSQSENKLTDAYLTKYPALVYSPILSVDYALNSK
                     ISFTLQGVYLIANYDNKFLYNPTLQFGILFNR"
     gene            complement(19879..19962)
                     /locus_tag="PKH58_03360"
     tRNA            complement(19879..19962)
                     /locus_tag="PKH58_03360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19926..19928),aa:Leu,seq:gag)
     gene            complement(19978..20061)
                     /locus_tag="PKH58_03365"
     tRNA            complement(19978..20061)
                     /locus_tag="PKH58_03365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20025..20027),aa:Leu,seq:cag)
     gene            complement(20112..20184)
                     /locus_tag="PKH58_03370"
     tRNA            complement(20112..20184)
                     /locus_tag="PKH58_03370"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20149..20151),aa:Gly,seq:gcc)
     gene            complement(20325..20753)
                     /gene="trxA"
                     /locus_tag="PKH58_03375"
     CDS             complement(20325..20753)
                     /gene="trxA"
                     /locus_tag="PKH58_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX88098.1"
                     /translation="MTTVLLITTVVILALTVLIAYSRYKLKNTPKVEDHEKIITLTDR
                     NFHQQIKGKIILVDFWATWCAPCRMQAPILNELVATDENNNAYYVGKVDVDANRQLAN
                     KYNIRSIPTMILFRNGKEISRFTGVKTKAFLLKEIRNIIE"
     gene            complement(20750..21217)
                     /locus_tag="PKH58_03380"
     CDS             complement(20750..21217)
                     /locus_tag="PKH58_03380"
                     /inference="COORDINATES: protein motif:HMM:NF020475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HNX88099.1"
                     /translation="MKDLYSKKDLKLMRAAEDSLLMGQNRVAELIKYAENTGVKRIGI
                     AHCVGMTREAMQLKERLAGKFEVYSVDCKYGKVPASEMLDDETARGTSCNPAGQANFL
                     AENNTELNISFGLCMGHDIIFNMKSKAPATTLVVKDREHKHNPYNEFKKTTES"
     gene            complement(21310..21750)
                     /locus_tag="PKH58_03385"
     CDS             complement(21310..21750)
                     /locus_tag="PKH58_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HNX88100.1"
                     /translation="MIEKYYQYNYKLASGAQPSEAEFAELKELGFEAIVNLSPASTRN
                     ALANEALIIEKTGMHYVHFPVDCSNLKKFHYTIFEGIMNGLKGKKVFVHCGGNIKSSN
                     LIHMYNVLANNQDERKSLETLYKIQLPEDKWFDYFKLMGMEGSR"
     gene            complement(21763..23031)
                     /locus_tag="PKH58_03390"
     CDS             complement(21763..23031)
                     /locus_tag="PKH58_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX88101.1"
                     /translation="MKEINASEFQKEVLKGGKVVLDFYSTECPPCEALAPKFEAMAEL
                     YGDKIKFVKMFRQGNRELATQLGVSSSPTLLFFEDGKQILDTISGGIKKAEIQERLNK
                     MISPEEVEAIKSRQKHTVSEYDVAILGGGPAGLTAGMYLGQAKLKTVLIDPALPGGYM
                     GITHQVSNYPGYVEPQPGFMLAHYMSEQAKQTGIDFKVAVDVTSVDLAKKEIVIDEFE
                     TIKARKIIIATGTSPNTMNAPGENELKGKGISYCATCDAKYFVDKDVTVIGGGNSAVE
                     EAQFITKFARKVTMVHQFDKLTANQTAIENLQANEKIRVMYEHEPRKFEKRGDKMITT
                     VENLKTKELSELEADGVFVFIGFKANVSVLGKTLPALDNWGYIIADEDKKTSLPDVYA
                     VGDIISKKYRQITTAVADGTIAAMSISKEF"
     gene            complement(23200..24534)
                     /locus_tag="PKH58_03395"
     CDS             complement(23200..24534)
                     /locus_tag="PKH58_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX88102.1"
                     /translation="MSTTQNSPLGVRGSKHNIIVPVKDSGDYFIVNPLYKSADFVSAE
                     ELNFLQNGLDANGEFSERNYLVEENEEKRAFKLAYLDFIDTREEDEIQLFFVPNYSCN
                     FACSYCYQEGYSPAESKMTTEVVDAFFNYIGTQFAGRKKYITVFGGEPLLPGESQRKI
                     IEYLLQKSTEANLDVAFVTNGYTLASYVDLLKTASIREVQVTLDGTKSVHDSRRYMKG
                     GQSTFDKIIEGIDRCLQNNISVNLRMVADKDNLGNLPELARFAIEKGWTASPLFKTQI
                     GRNYELHVCNSSPEKLYTRTELYERIYELIKVYPEIIEFYKPGFSIAKYIAENEELPS
                     PLFDSCPACKTEWAFDFTGTIYSCTATVGKKGEELGTFYPVVTKNEEAISQWQDRDIT
                     TIEKCKTCEVALVCGGGCASVAKNKNGSTNAPDCRPIKELLSLGAAAYLHRS"
     gene            complement(24857..25531)
                     /locus_tag="PKH58_03400"
     CDS             complement(24857..25531)
                     /locus_tag="PKH58_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX88103.1"
                     /translation="MLIENKPNKATDTISLKLSVQSINARYTSLSETSCCLSCGVAIN
                     HANVKLNEICLDLGSGRGNDVIRMAEAAGEGGFAYGIDLSDGMVSKARRNIEKFDIKN
                     AEIIQSEMEKLPLNENCIDVVISNCTINHSSDKEAVWSEVYRVLKPGGRFVVSDIYAT
                     LPIADEYRNDPEAIAECWAGAVTRAEYLFMLEEAGFSNIKILEESEPYAKGKAEVASF
                     TVYGEK"
     gene            25887..>26299
                     /locus_tag="PKH58_03405"
     CDS             25887..>26299
                     /locus_tag="PKH58_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX88104.1"
                     /translation="MLKKDLQPEFVQSLCEDSDKVLQFLADVKWEKGFVCHSCGHTNF
                     CSGKTLASRRCTRCKKEESATAHTIFHNCKFPVNKAFYIIYTICFEKKKHSVNELADE
                     LCVNPMTCWKFRTKIKKCIQKHQDTSEDEIDVKEIL"
BASE COUNT         7179 a         5427 c         5586 g         8107 t
ORIGIN      
        1 ctaatgttga aatgtttttt tcaccccgct atttgaaatt tcacccctca ttcagaattt
       61 ttatcccccg gcgtgggtgt tttccccttt tatttggcaa aaaaagacgg ggatatttcc
      121 tttgtaaaaa aaataagtca attaatgaac tattcgaagc gatattctga actaaacgag
      181 caatttattc ctggaaaaaa taaccgattg tccgatacaa atattgatat ccggtttatc
      241 ccttttgtga gttcggtggt caacagtgat gatgcagcat ggagtttcaa tccggctgac
      301 cgcgaaattg ctgaaaatat ttcgtttgaa tatcctgttt tcattccgcg gggtaaggaa
      361 aaatatgagc atgcaatact tttaatgcat ggcctcaacg aacgcagttg gaacaaatac
      421 cttacatggg ccgagtatct ttgcatcact accggcaaac cggttatact ttttcctatt
      481 gcatttcata tgaaccgttc gccgcaggcg tggaccaatc cgcgctgtat gcaacatgca
      541 ctcacttctc gacgaaaact atacggtgag gatcgctccc tgagttttgc caatatagcc
      601 ctgagtgaac gtatcagcaa aaatccgttg ttgttttaca gttccgggcg acagacagtc
      661 tttgatctgg agcaattata ccgggaaatc aaacagggaa tacacccgct tttcagttcc
      721 ggtgcgcgga ttgatatttt cggatattcc attggggctt ttttatcgca aatcgtactg
      781 atgtcaaatc cttcagggct tttttccgac acccgccttt ttatgttttg cggaggaggt
      841 attttcagtt ccatgtacgg cgtatcgcgc agcatcatgg atcgcgcagc tttcgatcat
      901 ctttatcagt attatctcaa tgattttgga ttgacgcaga aactcaaacc cgataaggtg
      961 tttgatgcat tttacagtat gatatctccc gataggaaca ggtcggaacg cgaagctttt
     1021 ttcgctcaga tgggcaatcg cctctgtggt attgcactcg aacaggattt ggttatgcct
     1081 tatgtcggtc tacaacaggc acttgggaca gattgttcca atgaaaaaat taacctgcag
     1141 gattttccat tttcttacac ccacgaaaat ccgtttccaa ttggtgctca taccaatcaa
     1201 atagctgtaa atgatgaatt taataatgtt ttttctcaag cagctggttt cctgacatga
     1261 tatggataat tattttttca atctgttttg tttttaaata gcataaaatt ataacctgtc
     1321 aatacaggtg ttttttcaaa tttatttttt tttttcaaaa aaacaatttc ttataaagaa
     1381 atattatatc tttgttgtga actaaacttt ttactttcaa caactaaaaa aaagtacaca
     1441 tgaaaaaatt attacttttt attccctgta ttatgttttg cttatcggca attgcagcca
     1501 attttactgt ggatggaatc tattacacca tttcgggtag caatgtgagc gttacctccg
     1561 gagcatatac cggagcaata acaatacccg ctactgttac ttacaatagt ataacttatc
     1621 ccgttactgc tatcggaaat atggctttcg cgtcctgcag tgatttaacc tcagtctcca
     1681 ttccatcctc agttacagct atcggagatg atgcttttta tcgttgcacc ggtttaacct
     1741 cacttgttat tccaacttcg gttagttcta tcggaaatta tgcttttgaa tatgcaggct
     1801 taacgtccat ttctattcct tcttcagttg gtactatcgg acaacatgct tttaattttt
     1861 gcacaagctt aaaaactgtt acaattcctg cttctgtatc atttattgga atagaggcgt
     1921 tttgggcttg tagtgcctta acgtcgattt atgtgtatag atcagtacca cttgatttga
     1981 cttcgtccga taatgttttt tatggagtaa ataaaacttc gtgtaccttg tatgttcctg
     2041 tcggcactaa aactgcttat caggcagcaa atcaatggaa ggattttgta aatattgtag
     2101 aatttactac tgccgtacca agagtagatg atgaacgcat aaatatacat ccaaacccgg
     2161 tagttgataa tttcaagatc gatggtttgg aagggactat tgatctaatc cttatggata
     2221 taaatggtaa agaattactt cataaagaga tattaaagga cgaaaatgta tctgtaagtg
     2281 aatttcctgc gggtatgtat tttgttaaac tgtcaaacaa cggattaaac tataaaaaga
     2341 aaattatcaa gaaataatta atatatataa atacaatcca cgatagctcc gatagctatt
     2401 gtggattgtg ttatatttta tatgtgaaat ttgcaatcta taatttattt ttaattgcct
     2461 tcttcacatt tcagatattt cattcttcat tataaatctt atcactgcac gataccaaac
     2521 agctttaatc taagctgaaa aaaaaacctg ttgtgtacat tctgtacatt caatttctca
     2581 aatttgattt tttaaaaact ctttttctct gaaaggaata ttgtatcttt gtcatgacgt
     2641 taacttcaag tgtaactaat tcacctacat ggaatgttgg ctgccattat aaaaaccaat
     2701 aacaattgac cgacacatga caattctctc taagaaagtt ctgtttattg accgcgatgg
     2761 aacaataatt actgaaccac cggttaccta tcaggtagat aacctggagc agcttgagtt
     2821 tttacccgcc gtaatccgga atctgtattt tatccgtcat aaacttgatt ttgaaatggc
     2881 attggttacc aatcaggacg gattaggcac cgatgtgtat cctcaggtaa atttcgatac
     2941 ggtgcagcga aaatttatgc aaacattatc aaacgaaggt gtttggtttg ataagatttt
     3001 catcgacaaa tcgtttcccg aagataatct tcctacccgt aaaccgggta cagccatgct
     3061 gaaaggatat tttaccgacg aatatgacat tgccggctcc tatgtcatcg gcgaccgcgc
     3121 aaccgatgtg gaacttgcca gaaatctcgg atgcaaagcc atttatctga gtaatgatac
     3181 cgaggaactg caagcgaaag gtttgacgga attctgcgct ttacagacta ctgactggaa
     3241 tcatataacc gaatttctgt ttgccggtga acgcgcggcc gtgataaaaa ggactaccaa
     3301 agagacagat attgaggtgg aaataaacct ggatggttcc ggtaaatcag aaattagcac
     3361 tggtctgcga tttttcgatc atatgctcga acagatagga aaacattcgg gtgctgatct
     3421 taaaatagaa gttaaaggtg acctggaagt ggatgaacac cacaccatcg aagatacagc
     3481 catcgctctg ggtgaagcgc tgcttaaagc tctgggcgac aaacgtggaa ttgaacgtta
     3541 tggatttacg cttccgatgg acgattgtct gtgctcaact gccctggact ttggcggacg
     3601 tgcctggctg gtgttcgatg ctgatttcaa acgagaatat gtcggcgatt tccctaccga
     3661 aatgctactt cacttcttca aatcgctgag cgatgcagcc cgcatgaacc tcaatatcta
     3721 cgctaagggt gaaaatgagc atcataaaat ggaaggcata ttcaaatcat ttgcaaagtc
     3781 aattaaaatg gctgtcagac gcgatattta taattatgaa ttaccaagta caaaaggggt
     3841 attgtaaatt ataataacta atcatcgaac attcaaaaaa atgcgaaaat ttttaatcct
     3901 gctttttagc ttcttatgca ttgtttctca ggctcaaatt aaaacggatg aacctccaag
     3961 actgttgatt ggcataaaag ttgacggaat gcaaaacagt catatacaga aattgtggaa
     4021 atatttttct gctggcggtt tccgaaagat aattactgaa ggtaccgtgg tagaacagat
     4081 ggaacatcct gtagtgtctg ccggtaattc ggctgatgtc gctacgattt ttacgggtac
     4141 tgtgcccttt tatcacggca taaccggtga ttattatttc gacagggaag ataaagaaat
     4201 aaaatccatt ctgtccgatg aaaatcaggt gggcatagga acccgtgaga aattttcagc
     4261 tcaccagatg ctcagctcca cccttaccga tgaactactt cttgccaccc cgcagtcgat
     4321 ggtttacagc attgccataa atcccgaaga tgccatcatg atgggaggac atatggcaac
     4381 cggagtaacc tggatggacg atagtatgaa caaatgggtg actacagcct attacaccaa
     4441 aggactttcg cgctgggctg acctgatgaa tgtgaacgga agttttaact ctatcgcttc
     4501 aactgactgg gtgccattca gttccatctc ttcttatcta aatccaactg taaagggttc
     4561 gaaatctgtt ccttttaaat ataacccgtt ggaaaagggg aaaaatccgg cacgatcaat
     4621 cctgcgaaat acaccggctg ctaacaacct cgttaccgaa cttgctcaga ccattttcga
     4681 caaggaagca ctcggtgcag acaaatatac agatgcacta ctcatacaat atacagttaa
     4741 agtgcccggt cagctcacat cttcactcac caatgccgaa caggaagaca tgtatctgcg
     4801 cctcgaccgc gatttgcaga acctggtttc atccatttct tcacgagttg gttccgacaa
     4861 agttgtattt tttcttaccg gaggaacaga agatttacat tcgccggtgg aactgggcaa
     4921 caaccgcata cctgcaggct acttcaatgc caatcggtcg ctgtctctgg ttaataccta
     4981 tctgatggct ctttacggac aggaaaaatg gatcagcggc tattatggaa agaatatttt
     5041 tttgaataaa aaaaggattg aagaaaaaaa actcaatttt gaggaagtga aaagcaggac
     5101 agcagaattc ctcctggaat ttgaaggtgt ccaggcagca tatcccatca atgatattgt
     5161 gaactttacc ggaaactcgg tggatgttcg ttccaaatac cggaactcgt atcataagac
     5221 tacagccggt gatattgtga ttacactttt accgggttgg gtggagattg atgataaaaa
     5281 tcaagtcgtc ggtacagcca atacaccaag ggtttacctg ccattttatt tgtatggtgc
     5341 cagagtaaaa ccacaaaaga tcagcaccga ctgcaaaacc acagacattg ctcccacact
     5401 ggcatattta ctcggagtgc cgttgccgaa tgcctgtatt ggaaaatcat tgatacctga
     5461 ttagttctga aatttcgaac aataggttcc tgatagtacg ttttgttgct taattagtta
     5521 ataaaatata aaacaagaca catttatatc cctgtcaacg tcaaacattt gacatgctca
     5581 catgaacact gatatttaat gtcgactgct attgaaaaat aatatgaatt acaattttga
     5641 tgaaattata gaccgccgca ataccggtgc cgtaaaaatt gaattatgta agaaaatgtt
     5701 cggtactgaa gatctgatac cgctctgggt agcagatatg gatttccgca cacctgattt
     5761 tattattgat gccattaacg aaaggtgtaa gcatcccatt ctgggctatt cggttcccga
     5821 tgatgagtat ttcgacagca tcatccggtg gattgaaacc cgtcacggat ggaaactgga
     5881 gcataattgg ctgggatttt tacccggtat tgttcccgga ctggcatttg cggttaacgc
     5941 attcacccgt gttgacgacg aaatcatcat tcaaccaccg gtttatccac cctttatcaa
     6001 tgttccctca aaaaatggca ggcagctggt gttcaatccc ctgaaagaag tggatggccg
     6061 atttgaaatg gattttgagg atttggaacg gaaaatcacc gggaaaacac gtctgttcat
     6121 cctgtgtaat ccccataatc ccggaggacg cgcatgggat gctgaaactc tgaccaaact
     6181 ggccgcaatt tgtcataaac acggcattct tgttgtgtcg gacgaaatac attcggatat
     6241 ggcattgccc ggaaatgttc ataccccgtt tgctaccgta tctgaacttg ccgaacaaaa
     6301 tagtatcacc tacatggctc cgagcaaaac attcaatatg gccggattgg ttagttcatc
     6361 ctacattatt cccaatcgtg atatacgaaa gaaattcagc gattttctgg aaaactccga
     6421 actggccaac ggaaatatct ttgcctatat tgccgcaaag gctgcctatg agaatggaac
     6481 tgaatggctg aatgagatgg tggactatat tcagggtaat gtggaatttg tggttgattt
     6541 tctcagaaat aacattccgc agattaaacc gatggttcct caggcatcgt tccttatttg
     6601 gctcgactgc tccggtatgg gtatgaatac caatcagatt cagaatttta tcatcaacga
     6661 agccggactt ggactaaaca aagggacaac tttcgggcct ggaggcgaat atcacctgcg
     6721 gctcaatatt ggctgcccga gagcaatact cgttgaagcc atgcaacggt taaaacaggc
     6781 aattgacagt aaataagatg tatgaaaata tcattgcagg taataatatg aaaaatattt
     6841 tatatatatt tctggctctt ttatgtataa cagaaacctt ggcacaaacc agaaaaagtg
     6901 ttgaggctta tctgatttct tcttcaatga ctattgatgg aaaattgaac gaaccggttt
     6961 atacacaagc cctacctgca aaagattttg ttcagttaca accttataac ggaaaacctt
     7021 cagtaaaacc cactgaagtt tatttctttt acgaccagac cgcgatttac gtaggtgcta
     7081 aactctatga tgatcccgac agtatattca actatctgac cgaacgggat aatacgggaa
     7141 tgtccgatta tttcggtgta tatatcgatc catacaatca ggggcagttg gcttttggct
     7201 tttttatcac tcccgcgggt gtacaggtag atgttaaagc tacaaaaagc gacgaagata
     7261 ttgaagatcc caactggaat gctgtttggg aaagcaaaac gcagattaat gccgacggat
     7321 ggacggtaga gatgaaaatt ccttactcgg cgatccgttt ttcaaaaaat gctaataaca
     7381 gttggggatt gaatatgttt agaaatataa agcgtcacaa ttccaacaat tcctggaact
     7441 ttattgaccg taaagtctct ggttttattc atcaggaggg ggaacttatc ggcatcagca
     7501 atatcaaacc gcctgtcagg ttatcgctaa gcccgtatct tgcaacctat tttcagccaa
     7561 aatcaaaaga tgcttcagcc ggatttttat acaagggagg attagacctg aaatatggat
     7621 taaatgatgc atttactctg gacatgatgc taattcctga tttcgggcaa attcagtcgg
     7681 acgacagaca gttaaatctt tcgccctacg aactttatta tgacgaaaag aggcagtttt
     7741 ttaccgaagg taccgaactc tttcagcggg gtggaatttt ctattcgcgc cggattgggg
     7801 cagcaccaaa gttttcggca tctggtcatt tgaatgatgg tgaagtcgtt gattacaatc
     7861 ctgctgaaac tcaactcgta aacgcaacga aagtatccgg acggacccaa aacggatggg
     7921 ggataggtgt gctcaatgcc atgtcgctgc cttccgaagc caccctgaaa aatgccgaaa
     7981 cgggaagtac gcgcgaagtg caagtacaac cgtttaccaa ctacaacgtg gcggtggttg
     8041 ataaatcgct gaaaaataat tcatatatca gtcttatcaa taccaatgtg ttgatggcgg
     8101 gtaatccgtt tcatgccaat gtgacggcta ctgaatttca gttccgggat aaaacaaaaa
     8161 cttatgcgct gaaaggaaaa gcgggtgtca gtgtccgtgg taacgaaacc atcgaaacgg
     8221 gtttttacag tacgctgagg tttgaaaaga ataaaggaaa ctggttgtat ggagctgctg
     8281 aaaatttata ctccgataag tttaatccga acgatttggg atacctggaa cgcaataacc
     8341 tctccaatac tgaagcctgg ttttattatc aggttattga accgaaaggg attttactgg
     8401 aatggaactt tgggctttgg agtaactaca accggatata taacccgaat acaatcttcg
     8461 ataccgaagc aggttatgaa gcttccgcgc gatttaaaaa taattttttc ctgtattcga
     8521 acgctaatgt tgcttcccgc cagaacaatt actatgaacc acgcgtgaaa ggacactatt
     8581 atctcaatcc cgtaaattat aaattcaata ttcagcttac atccgataca cgaaaacatc
     8641 tattttttca aggaggatac ggttatcgcg tacaaccaga aataggcagc gtagaacatg
     8701 ccgctgaatt ggaatcttat ctgaggatag gacaacggtt tgtttttgac tattcaatag
     8761 gtggtaataa atcgaataat gaacgcgggt atgtgatgaa taatgcagcc ggtgacagca
     8821 tcacttttgc acgccgcgat gtgtccacgc tcatcaacac cctcagcgct tcctatatct
     8881 tcaattacaa aaccagcctt actttcagaa tgagacatta ctggtcggga gtgagtaata
     8941 aggaattttt cctgttgaac gatgatggta ccctcaatgc atatccggat ttttcacaga
     9001 atatggatca aaactacaat gcatttacca tcgatatgat tttcaggtgg atatttgctc
     9061 ccggctccga actttcgctt gcctggaaaa ccaattcata cgacaatcat aatgaagtga
     9121 tttataatta tcaggataac ctgatcaaat cctggctcaa ccagaagaac agtatatcgc
     9181 tgaaagtatt gtactatata gattataaca gacttaggaa aaaggaaata taatgaatga
     9241 cttaaaagag aattattgtg gaagtaaaaa ttgaagaatc ctggaaacaa agattatctc
     9301 ctgagtttga aaaaccctat tttatagctc ttactgagtt tgtgcacgcc gaataccgcg
     9361 ctaaacagat ttttccgcct gcaaaactta ttttcaatgc atttgacagt tgccccttcg
     9421 ataaggtgga agttgtaatc atcgggcagg acccttacca cgaaccgggt caggctcatg
     9481 gattgtgttt ttcggttaat gatgggattg atattccgcc ctcgctggtc aatatcttta
     9541 aagaaataga acgcagtctg ggcaaaacca tcccaacatc gggaaatctc actcgctggg
     9601 cacagcaggg tgttcttttg ctgaacgcga ccctaactgt gcaaaatcac aaagcaggtt
     9661 cacatcaggg aaaaggctgg gagactttta ccgatgcagc catccggaca ctggccgctg
     9721 aaagggaaaa tctagtcttc ctgctctggg gcaactacgc acaacaaaaa ggtgctttta
     9781 tcgacccatc gaagcatctg gtgctcaaat ctgtccatcc ttcaccgctg tcggcttacc
     9841 gcgggtttat cggcaacaac cattttgtac tggccaacaa ctacctgaaa gaatacggga
     9901 aaacagaaat tgattggtag attcacgttt gacgtttgat atttcgggtt tcacgtttga
     9961 tgcttgttaa ttgacgttct catattccaa gattattaga gccaggaacc tggactattg
    10021 taactatgaa ttcatcattc acaaatcata cctcatcaat taccgctttt gtcagaataa
    10081 aaaaaaatat tatctttcgc aaaatattta aataccagaa attatgaaat ggattaaccg
    10141 aaatgacgat gagtcaaatt atgaagatcg caggggcagg tcgaccggaa gaagactggc
    10201 agccggtggc ggcataggaa caattattat tcttgctctc tatatgttta cagggcagga
    10261 tttttcttcg gttgcagata tgtttacagg cggagaagct cagactgaaa ccgttgaccc
    10321 atcacgtgct aaggaaaacg aagatctgaa ggttttctca ctcggggtat tcaacagcgc
    10381 caacgatgtc tggacgaaaa tattcaacga acaaatgcag atggaatacc gtaaaccaac
    10441 ttttgtaaca tttactgatc agactatgtc ggaatgcgga ggtgcaagcg gtgcaacagg
    10501 acctttttac tgccccggag acgaaaaggt gtatatcgat ttatccttct ttcacgaact
    10561 agccacccgg tttaaggctc ccggcgacct ggcaatggcc tatgtaactg cccatgaggt
    10621 gggacatcat gtacagaaac tgcttgggat aactgataaa atgcaacaat accgcggacg
    10681 aatttcggag gaggagtata atcagctttc ggtcaggctg gaattgcagg ccgatttcta
    10741 tgctgggctt tgggcgcgat atgccgaagc catgaacatt atcaaacttg aggaaggtga
    10801 tattgaatcg gccctcggag ctgccaacgc cgtgggcgat gataccattc agaaacaggc
    10861 tcagggttat actgtacccg attcattcac tcacggcact tcggcacaac ggatgcgctg
    10921 gtttatgaaa ggctatcaga cgggagatta ccggcaaggc gacaccttca atgcagagac
    10981 attataaaag ctaggactga tagagcaaag actatcagaa caaagaacaa agacttttag
    11041 tatcaggaca tttagagtta agatacatgg caataagagt cagataccag gactgataga
    11101 acaaatcact aatcacagcc ttttcttgaa tcccctttgc ttgtccctgc ccgtcccgaa
    11161 tttatatcgg gaaacttgtt acgggaaggg gggcaggggg cttaatcatt caaattccac
    11221 ggttcgtttt tcacggttgc ttttaaaggc gagttcgata atgcggatta cgtttcgggc
    11281 ttcctcgggc tttacggcca gttgggcaaa gccgtggatt gcgtcgcgca ggttgatgaa
    11341 atactcgcgg taatcgcctt tttcactttc cacgtatgcc tccatcatca ccccttcaat
    11401 gtcggtaacc agttttccac gaatgctttc gggctctttt ccccagtcgg gggtgttcgg
    11461 gatcatgcct ttgttcaggt tttcttcctg aacatccagc ccgtatttga caaaagagcc
    11521 tttcgtgccg tgaatttggt aagtgggacc gggtatccgg gctatcattg atgatttgag
    11581 ggtggccgtg tgagtggggt agtagagttt cacatcaaag cagtcgtcgg tttgggccca
    11641 ctcacgctgg gtactgatat gagcagtaac tgcctgcggc aatccgaaaa aatggagcgc
    11701 ctgatcgatg aggtgcgagc ccagatcata gaatttcccc gaaccgggca atggattctc
    11761 ccgccagtat cccgtgggac gcggttgagg acggaaacgg tcgaaatgcg tttcgtaatc
    11821 gaccaaatct ccaagaatac cgctgtttaa aatctttcgg accgttttgg tatcgctcac
    11881 aaaacgtcgg ttgtggtagg cggtcagtat tttgcccgtt tcacggctta gccggataag
    11941 ttcatcggct tcagcagtgg tgttggtaaa tagtttctct accaccacgt gcttgccagc
    12001 gagaagcgct tcctttgccc actatcaatg atacctgtgc gaatattatc catgttatat
    12061 tatattttta atgataattg aattaaaccc gtaacgatcc gcgaaatccc ctgaccgcat
    12121 agtacgattc tgctccgttg tgataggtaa acacatgatt gtagcggcgg tcgcagaaaa
    12181 tagctcctcc catacttctg atttcagccg gagttttcac ccaactggag gtcttgaggt
    12241 cgaactcacc cattttttgc aactcacgat attcatcttc cgataaaagc tcaataccta
    12301 tttcggctgc catatccaca gcgttgtttt tgggcttgaa ttcttttctg gattcgagcg
    12361 cttcgcggtc gtagcaaagg cttcgccggc ctttaggcgt ctcggccgag caatccacaa
    12421 agatatattc accggtggcg gcatcatgac ctgtcacatc gggttcgcct cccgttgttt
    12481 ccatttgatg gagcgaaaat agtttttcgg cattcgcttc cagcttttca tgaattttct
    12541 cccattcaat attcggatgt cgttgtaggt ttttctcaaa tcgttttttt aacacgtcga
    12601 gaaattccgc tctttgccgc gatgtcaggt tcattgagtt taccattgag gtagcatgaa
    12661 attattcttt tattaaaacc agatattata gaaaatagtt taagcgtgtc tgaaataaaa
    12721 gcctcacctt tatttcccga atatgtttcg ggaggttagg ggattgagac ctcaaagtta
    12781 aaaaaaataa gtacaagcga aaacctgtcc ttaaaaaata tgtttttggc cagtctcgat
    12841 tttcgatgtc gggacattga tttttgatgt ctgaacattg aattttgatg tcacattatc
    12901 tcaccgatat tgcaatgtac cgttccaatg ctcctttgca gagagtgtca gggaaactgt
    12961 gtaaataaaa aaagcagaga aacggtttct ctgctttcat acggtaaata tgtaacgggg
    13021 cggtttattt catgatgagc atctgctgtc tgaccgactc ttcatggatg gccattagta
    13081 cggttttcat aaaatcgccg ttcataccca ttttttcggc ctgatttacg cggtttttca
    13141 gaatttcgtc gtagcgctga gcttgcagga tgggcatgtt atgttctttt ttgtaggtcc
    13201 cgatttcctt cgatacccgc atacgtttgg caaggagttc aagcaggtca ttatccagac
    13261 tgtcgatctg ccgtcgcagc acggtgaggt tttcggtggt ctggttggtt tcgcggatga
    13321 ccagcgagtc aagaatgagt tgaagtattt cgggagttat ctgctgcgcg gcatcgctcc
    13381 aggctttgtc gggggtgcag tgcgcctcga tgatgaggcc gtcgaaattc aggtccatag
    13441 cctgctggct gatgggcgca atcatttccc ttttaccgcc gatgtgcgag gggtcgcaaa
    13501 tgatgggcag ggtggggagt tggcggtgca actctatggg gatatgccac tgtgggatgt
    13561 tgcggtatat ttttttgtcg gacgaactaa aaccgcgatg tattgctccg atgcgacgga
    13621 tgcccgcgtt gtaaatccgt tcgatggctc caatccacag gtcaaggtcg ggattcacgg
    13681 ggttcttgat aagcaccggt atgtcggttc cttcgagggc gtcggcaatt tcctgtaccg
    13741 aaaatgggtt tacggtggtg cgagcgccta tccagagaat atccacatcg ttagccaggg
    13801 cttcgcgcac gtgcctggca ttagctactt ccactgctgt gtacatgccc agttcggatt
    13861 ttacacgttt cagccaggtg agcgcttctg cacccacacc ttcgaagcca cccggtttgg
    13921 tacggggttt ccagactcct gccctgaaaa tttttacacc tatattcgat aatgctttgg
    13981 ctgttgccat tacctgttct tcggtctcgg cgctgcatgg ccccgaaata atcaggggtc
    14041 gcttcgggtc aacgcccggt agtaatattg attgtagttc cataatgaag ccccctctaa
    14101 ctccccctca tgggggagaa taacttccgc gagaggttaa cggttatgat taatttgtaa
    14161 atgtttttta aaatttatcc tgtaaattat ataaacctta tatgttccct gtctggttag
    14221 attgtaattc ttctgatcac ttcctccagc atactttcgg ggctgcaaag cgagatgcgc
    14281 aggtatcgtt ttcccttgtc gccgaaaata aaccctgggg tgataaatac atttttttcg
    14341 tacagaattt tgtctgctat ttcgcctgcg tcctgccatt tatctgctat ttttccccac
    14401 acgaacatgc ccacctggtt tttgtcgcag gtacattcca gtgtatccat aatacggtac
    14461 gccagttcgc gtcggcgggc gtacacctca ttcatcttat cgtgccattc atcagtgttt
    14521 tgcaacgctt gcacggcggc taccatcatg ggacggtaca tgcccgagtc gacgttcgat
    14581 tttacgttca atacccattt cacaaactgc tcattacttg ccagcatggc catacgccat
    14641 ccggccatgt tatgcgattt gctcatcgaa ttgagttcga tgcagatgtc tttggctcca
    14701 gggatggaga ggatgctctg cttccggtcg ttgaggataa agctgtaggg gttatcgttg
    14761 cagatgacta tgctatgtcg ttttccgaaa gcaaccagtt tttcgaacag ttccacactg
    14821 gcattggcac ctgtgggcat attgggataa tttacccaca tgagttttac attcgataag
    14881 tccgtttttt ccagcgcgtc gaaatcgggt tcccagttat tattttcgtc cagatcataa
    14941 ctgatgatgt tggcttcgca aatgcggctg gcgctgctgt aggttgggta tccggggttt
    15001 ggcaccagca ctccgtcgcc ggggttaaca aaagctagtg tgatgtgcag aataccttcc
    15061 ttcgatccga tgagcggttg aatttcagtt ttcgggttga gttccgtatt gtaccagcgt
    15121 ttataccagg cggcaagtcc gtttcttagt tcgggaattc cggtgtagct ctggtaaccg
    15181 tgaacgttgg gttttcgggc ttcggtgcag agtgcatcga tggtctcttc cgaaggggga
    15241 agatcggggt tgccgatgcc caggttgagc acatcctttc cctgagccct catctcatct
    15301 atttcgcgca gttttttcga gaaatagtat tccgaaacac tgttgagccg gttagctggt
    15361 tggattagtt cctgtccacc agccccctca aaggggagca aagaagcatt atttgaagtt
    15421 tgtgacattg ttatatatat tactgtattg tttttctatt gcattaatat ctaatcagta
    15481 actctcttaa ttccaccttc agggggttag gggggtaatt tgtgaattat ttagtcttcg
    15541 ggagtttttc cttcgcggta ttcgcccagt atctgaaact ctttggtcag aggttttatc
    15601 gcatcgagcg attgccggta gcgttcataa tcgtcaaagg tcaggttgat gtaaaactga
    15661 tattcccact cccgtccgag gatgggcagc gactgaattt tggtcagatt catgtggtaa
    15721 aaagaaagga tagaaagaac tttcgacagg ctgccttctt cgtggggcag ggtgaacacg
    15781 agggtggatt tgttcagcgc tatttgtctg ttcattttat aagccaggtc tggtttggca
    15841 atgatcagga accgcgtgaa gttgcgttta ttcgtttcaa taccttcagc gatgatttca
    15901 agtccgtaga gttcggctgc cagtcggctg cagatggctg cccgtccgga tattttattt
    15961 tcggcaattt ccttagcaga gagggcggtg tcgtcgcttt ccacagctcg gaggtttttg
    16021 tgcgcgtcca gaaaatcttc gcattgcatc agcgcgatgg ggtgggagta aacctcatgt
    16081 atatcatcca tttcttgtcc cggtagcgct gccagctgat gcgagatacg cagtttgtat
    16141 tcacctatta tcaggcagcc gctttcgcgg agcaggttgt gattttggag caggctaccc
    16201 gctatggtgt tttctatggc aataattccc aacagactct cgtcgtcgtc aattgctttg
    16261 aatacgtctt tgaaggttgc acagcagact atttccacat cttcatcctc aaagtatcca
    16321 cgggcagcta tttcgtggaa tgctccgctt actccctgta tggctattcg ttttgtcata
    16381 agttattttt tgtttgatac atattgtata aaaaaatcct gcccgaagac ttcgagcagg
    16441 atttttaata tcgtataatt cgtttattta tttcaaaatt tacatatatc gtatcctgct
    16501 ctcacctgct aaagtaaaag taaaaataaa aatatgcaaa ccaggtattc attttagaaa
    16561 tatagatatt ttgaattgaa cggtacaaaa gtaatacaat tttcaaaaat aatatcataa
    16621 tgtctgattt ttttcaaaat aaattctaaa atgttgtttt ttctggtttc tttataagat
    16681 ttcatttaat taccactatc atagttgctt ttaagataga taatatagtt ggccgattat
    16741 tgtgcttcgt tacttttatt aatgattgct aattattgaa gtttttcatc cagtaagtta
    16801 aaagatataa caagaaatat ttattatctt tgaagccaga aaaaatcaat caatatctta
    16861 acaataaaaa ttaattctat gactaacaaa attgaaaaaa agagctacgc gttgccaatt
    16921 gcgatgatgt ttgctctgtt ctttatgatt tcattcgtaa ccggcttgcc aagtccaatg
    16981 ggggttattg tggccaaaca gtttggcgca tcaaacttcg agtctcagtt aggatttttg
    17041 gctaacttca ttgcctatgc gtttatgggt attccggcag gtctgatgct caaaaaaatc
    17101 ggttacaaaa aactggctct cgttgcagta actgttggtt ttacaggagt tggcattcaa
    17161 tggttatcag gtcaggttgg aagttttacc gtttatctga tcggtgcttt tgttgcaggt
    17221 ttctccatgt gtatgcttaa tactgttgtt aacccgatgc ttaataccct cggtggtggt
    17281 ggtaacaaag gaaatcagct catccagtgg ggtggtacca ttaattcatc tgctgctaca
    17341 atcgttccta ttcttgtagg ttatctcatg ggcgacgtag ccggacgtac tatcgacaaa
    17401 gcaagccctg ctctgtttat tgctatgggc atttttgctt tagcattcat tgttctgttt
    17461 atagttgata tacctgaacc tcacattcag aaagtatctg aaaagaaagt aaaagatacg
    17521 cacagtccac tttctttccg tcactttatt ctgggagcta tcgctatttt catttatgtt
    17581 ggtatcgaag ttggcattcc aagtactgcc aatctgttca tgactgctct tccttcggct
    17641 gcgaatcccg gtttgggaat tgacgcaggt actgcaggta ttgttgtagg tatttactgg
    17701 ctgcttatgt tggttggacg tttggttggc gcatcggtag gagctaaaat ctccagtaaa
    17761 acaatgttga cttttgcttc ttcggtagct atggttttca tcctgctggc tatttttatg
    17821 ccggtatcaa ctacaatcaa tatgccggtt ataaccaaca cattctcttt tgctcaggtt
    17881 caggtgcctg ttaatgtgtt atttattatt ctttgcggtt tgtgtacttc cattatgtgg
    17941 ggtggtatct ttaacctggc tgtcgaaggt cttggtaaat atacagctgc tgcttccgga
    18001 ttattcatgg ttatggtttg cggtggtggt ctcattcctg tattgcaggg atttgtttcc
    18061 gacaattttg gcttcctttc cagctattgg gtaatgttcc tttgcctcgc ttatctgtta
    18121 ttctacggag taatcggttc taaaaatgta aataaggata ttcctgttga ataatttata
    18181 aaaactatta tttatgacta tggaaatgga aaaaccttat gtaataggta ttgatatggg
    18241 tggtacgaat accgtttttg gtattgtgga tgcccgcggt aatgttatag ctaaaggcgc
    18301 gatcaagaca gataatcctg atattaattc atacatcagc gaccttaaaa ctgaacttga
    18361 taaacaaatc gaagttgttg gaggtattga aaaaattaaa ggaattggag cgggttgtcc
    18421 aaatggtaac ttctataccg gaaacattga gtttgcccca aatctgcctt ggaaaggcgt
    18481 agtacctttt gccaaacttc tacaggatac atttggtgta ccggcagcta ttaccaacga
    18541 tgccaacgcg gctgctgtgg gtgaaatgac ttatggtata gcgcgcggta tgaaaaactt
    18601 tatcatgatt acactgggaa ccggtgttgg cagtggcatt gttatcgatg gaaaaatcgt
    18661 gtatggtcac gatggtttcg ctggtgagct tggtcacgtt cgcgttgttc gcaataacgg
    18721 acgtgtctgc ggttgcggac gtgaaggttg tcttgaaact tatacttctg ctactggtgt
    18781 ggcacgtact gcccgtgaaa ttctggaaat gtgcgatact ccaagtgtat taagatctat
    18841 agagttaaag agcatcacct caaaagatgt atatgatgca gctatggaag gcgatgaaat
    18901 agcaaaagag attttcgaat ataccggaaa aattcttggt gaagctttgg ctgattttat
    18961 tgccttcagt gctccggaag ctatcgtgtt gttcggtggc ctggctaaat ccggcgattt
    19021 aattctgaaa cccgtagtgg ataacatgga gaaaaatctg ctatctatct ggaaaggtaa
    19081 agtgaaagtg ctgttctctg cactgaagga agccgatgct gccgtactgg gagcaagcgc
    19141 ccttgcctgg gaactctaaa tcatttcctt tataaaataa gtaagccggt tcattgttgg
    19201 accggctttc ttattatctg ttgaataata taccgaattg cagcgtagga ttgtacagga
    19261 atttattgtc ataatttgca atcaaatata ctccctgaag tgtaaacgag attttggagt
    19321 tcaacgcata gtcaacactc aggatggggg agtaaaccag tgccggatat tttgtcagat
    19381 atgcatcggt gagcttattt tctgactgtg attggacatg gagatttttc accgagccca
    19441 tacctgcaaa tccggacagt gtgtacctga atttgcccga tttatgactt accccggcaa
    19501 acggtccgcc gtaacctaaa ctgatatatg aattatcaga accttcggta atgtatttcg
    19561 ttcgctgact tccaccataa attccggcag tgaaataatc ggaaatataa aatcgcaaaa
    19621 ttccacctat ggagttacta ttgttcttca aagtctgata atcggtactg gtcgttgtgt
    19681 agccgggctg ataaaacaac ataccaccgc tgtacagatt gcttttgttg ttttgtgaaa
    19741 aaacatgcat tgtgattagc aaaaatagta aactcatgac gtgtatcttc attgtggata
    19801 gttatttttg tgtatttcag aaggaattgg tgaaaaaaaa taatccctga cagtttgtca
    19861 aggatcgtta tcaggaagtg cgcaagatga gactcgaact cacacgccgg aaccggcact
    19921 accccctcaa agtagcgtgt ataccaattt caccacttgc gcatttagaa ttatttgtgc
    19981 ccaaaacagg actcgaacct gcacagcctt gcggccacta gtccctgaaa ctagcgtgtc
    20041 taccaatttc accatttggg caaatgatca gggagaaaaa aacgaaacgt taggtttcgt
    20101 ctgttttttt gagcgaaaaa cgggactcga acccgcgacc ctaaccttgg caaggttatg
    20161 ctctaccaac tgagctattt tcgcaaaaac ttatttttaa ttgcggtact tcccaatcac
    20221 tctcgggata tcgtttcctg caattgcggt gcaaatgtaa gcattttttt tagtcggtgc
    20281 aaatgttttt ttgaaaaaac gagtaaaaat cccctcagat ggcattattc aatgatattt
    20341 ctgatttctt tcaaaagaaa tgcctttgtt tttacaccag tgaaacgcga aatttcttta
    20401 ccattccgaa ataaaatcat tgtcgggatg ctgcgaatat tatatttatt tgccagctga
    20461 cggtttgcat ccacatctac cttgcccaca taatatgcgt tattgttttc atcagtggct
    20521 acaagttcat taagtattgg ggcttgcatc cggcagggtg cacaccaggt tgcccagaaa
    20581 tctaccagta taatttttcc ttttatctgc tgatgaaaat tccggtcggt cagggtaatt
    20641 attttttcat gatcttccac ttttggtgta ttttttaact tataccggga atatgcaatc
    20701 agcacagtta acgcaagaat gactacggtt gtaattaata atactgttgt catgattcgg
    20761 ttgttttctt gaattcgttg tatggattat gtttatgttc gcgatctttt accacgagag
    20821 tggttgctgg tgccttcgat ttcatgttga aaataatatc atgccccatg cacaatccga
    20881 atgaaatatt cagctcagta ttgttttcag ccagaaaatt tgcctgacca gccggattgc
    20941 aggaggtacc gcgtgccgtt tcgtcatcca gcatttcaga tgccggcact ttgccatact
    21001 tgcaatccac ggagtaaact tcaaactttc ctgcaagccg ttccttcagt tgcatggctt
    21061 cgcgtgtcat tcctacacaa tgcgcaattc caatacgttt cacccccgta ttttccgcat
    21121 attttatcag ttcagccaca cgattctgac ccatcagcag cgagtcttca gcagcccgca
    21181 taagtttcaa gtcttttttt gaatataaat ctttcatgcc ctcttaatac ttattggaaa
    21241 tttcaataaa atcagctaat gtgctatttc aattttaatg accaaaaact ataaactatt
    21301 gactattaac tatctgcttc cttccattcc catcagtttg aagtaatcaa accacttgtc
    21361 ttcgggtagc tgtatcttgt aaagtgtttc caatgatttt ctctcatcct gattgtttgc
    21421 aagtacatta tacatgtgta tcaggtttga cgattttatg ttgccgccac agtgaacaaa
    21481 aacctttttg ccttttaatc cgttcataat accttcaaat atggtataat gaaacttttt
    21541 cagattggaa caatccacag ggaaatgaac gtaatgcata ccggtttttt caataatcaa
    21601 tgcttcattt gccagcgcat ttcgggtact ggccggtgaa agattaacga ttgcttcaaa
    21661 acccagttct ttcagttcgg caaactctgc ttcggaaggc tgggctcccg aagcaagttt
    21721 gtagttatat tgataatact tttcaatcat gacctctaat acttaaaatt cttttgaaat
    21781 tgacatagct gcgattgtgc cgtcagcaac agcagtcgtt atctgacgat atttcttgct
    21841 gattatatcc cctacggcat atacatcggg caaacttgtc ttcttgtctt cgtcagcaat
    21901 tatataaccc cagttgtcca gagcgggcag tgttttgcca agtacggaaa cgttggcttt
    21961 gaaaccgatg aatacgaaaa caccgtcagc ttcgagttcg ctcagttctt ttgttttcag
    22021 attttcaacg gtagtaatca ttttatcacc ccttttttcg aattttcggg gttcatgttc
    22081 gtacatcact ctgatttttt cgttggcttg cagattctct atggctgtct ggttggcagt
    22141 cagtttgtcg aactgatgaa ccattgttac ttttctggca aatttggtga taaactgtgc
    22201 ttcttcaacg gcagaattcc cgccgcctat gacagtaaca tctttatcca caaagtattt
    22261 ggcatcgcat gtagcgcagt aggaaatgcc tttccctttc agctcgtttt cacccggagc
    22321 attcatcgtg ttgggcgatg ttccggttgc aatgatgatt ttccttgctt ttatggtttc
    22381 aaattcatca ataacgattt ctttctttgc aagatccacc gaagtcacat ccactgccac
    22441 tttaaagtcg atgcctgttt gtttagcttg ttcgctcatg taatgtgcca gcataaatcc
    22501 cggttgaggt tctacataac cgggatagtt tgaaacctga tgcgtgatgc ccatatatcc
    22561 tcccggtaaa gcaggatcaa tgagtactgt tttgagtttg gcttgaccaa gatacattcc
    22621 ggctgttaaa ccggccggac caccgcccag aatggctaca tcgtattccg atactgtatg
    22681 cttctgacgg gattttatag cttcaacttc ttcgggactg atcattttat tcagccgttc
    22741 ctgaatttcg gcttttttaa ttccaccact gatggtgtcg aggatttgct taccgtcctc
    22801 gaaaaacagc agagtaggtg aggagcttac gcccaattgt gtagctagtt cacgatttcc
    22861 ctgacggaac attttcacga atttgatttt atcaccgtat agctcggcca tggcttcaaa
    22921 ttttggcgcc aaagcttcac agggaggaca ttctgtcgaa taaaagtcaa gtactacttt
    22981 acctcctttg agtacttctt tctgaaattc agatgcatta atttctttca taaaaattta
    23041 ccaaccctga tccctgagca gggaaaaagg gattttaatt aaatgttgta tgttattgaa
    23101 taattttttc tctactaaac aaacagaaat gatatatgtt catttgttca tataatgtgt
    23161 tgtaatttct ttctaatata tagattattt cagtttttat caggatcgat gcagatatgc
    23221 tgctgctccc aagctcaaca attcctttat cggtctgcaa tcaggagcat tggttgaacc
    23281 atttttgttc ttagcgaccg acgcgcagcc tccaccgcaa accaaagcta cctcgcaggt
    23341 tttgcatttt tctatagtgg tgatgtctct gtcctgccac tggctgatgg cttcttcgtt
    23401 tttggtcact acaggataga aggtacccag ttcttcacct tttttaccca cagtagctgt
    23461 gcacgaatag atggttcctg taaaatcaaa cgcccattcg gttttacacg ccggacaact
    23521 gtcaaacagg ggtgagggta attcttcgtt ttcagctata tattttgcaa tggaaaatcc
    23581 gggtttgtag aattcgatga tttccgggta aactttaatg agttcgtaga tacgttcgta
    23641 caattctgtt ctggtgtaga gcttttctgg tgaactgtta catacgtgca gctcataatt
    23701 gcgtccgatc tgtgttttga acagcggtga tgctgtccag cctttttcga tagcaaagcg
    23761 tgccagttca ggcagatttc caagattgtc cttatccgca accattcgca ggtttactga
    23821 aatgttgttt tgcaaacaac gatcaatgcc ttcaatgatt ttgtcgaagg tggattgtcc
    23881 acctttcatg tagcggcggc tgtcgtgaac actttttgtg ccgtcaagag ttacctgcac
    23941 ttcgcgaatg gaagccgttt tcagcaaatc gacatatgaa gccagtgtgt atccgtttgt
    24001 gacaaaagcc acgtccagat ttgcctcagt cgatttttgg agcagatatt caatgatttt
    24061 tcgctgactt tcgcccggaa gcaatggctc gccaccaaac acggtaatat atttttttct
    24121 cccggcaaac tgagtgccga tgtaattaaa aaacgcatcc acaacttcgg tggtcatttt
    24181 gctttcggca ggactgtaac cctcctgata gcaataggag caggcaaagt tgcaggagta
    24241 attcggtacg aaaaacaact gaatttcatc ttcctcgcgg gtatcaataa aatcaagata
    24301 agccaattta aaggctctct tttcctcatt ttcctcaacc aggtagtttc tttcggaaaa
    24361 ttcaccgttt gcatccagtc cgttttgaag aaaattcagt tcttctgctg aaacaaagtc
    24421 agcagatttg taaagcggat taacgatgaa ataatcaccg ctgtctttga ccggtacaat
    24481 aatattgtgt ttggaccccc taactcctaa aggggaattt tgagttgtgc tcactttatg
    24541 aattatttat tctacttgta atttcctttt tatcttccat aggttaacat taaaattcgg
    24601 tactaccaaa ttttaggatt ttagataagt ccctaaagga gagtattgtt cccctttagg
    24661 ggctaggggt tgtttagtcg tgacaaccag caacaaactt cgaaactttg gcacagcatt
    24721 gcgcttgttt cggttgtttt gcgtttttag ttacactaac attttttact acacttttca
    24781 ttttctttga attataattg atttatatag cttcataata tttcctcttc aagcgttaag
    24841 gtgccgtcaa aaatctttat ttctccccgt ataccgtgaa actagccact tcggcttttc
    24901 ctttggcgta aggttcgctt tcttccagta tttttatgtt gctgaaaccg gcttcttcaa
    24961 gcatgaagag gtattcagcg cgtgttaccg cgcctgccca gcattcggca atggcttcag
    25021 gatcatttct gtattcgtcg gcaattggca aagtggcata aatatcgctt accacaaatc
    25081 gtccgccagg tttcaacacc cgataaactt cgctccatac agcttctttg tctgaactgt
    25141 ggttgatggt gcagttggat ataacgacat caatgcagtt ctcgttcaga ggcagtttct
    25201 ccatttcaga ctgaataatc tcagcatttt tgatatcaaa cttttcaata ttcctgcgag
    25261 cttttgacac catcccgtcc gaaagatcaa tgccgtaagc aaaaccgccc tcaccggcag
    25321 cttctgccat ccggatcaca tcgtttccac gtccgcttcc aaggtccaga cagatttcat
    25381 tcaactttac gttcgcgtga tttatagcaa caccgcagga cagacaacag ctggtttcgc
    25441 tcagacttgt gtatcttgcg ttgattgact gtacgctcag ttttaacgaa atagtatcgg
    25501 tagctttatt tggtttattt tcgatcagca tcgttgtttt agctttgatt ttgtcattaa
    25561 ttttttattc cgaataattc ggtgcaaata tatgaataat ctttcatatt acaatagtat
    25621 cacttgcttt tacataatat tttttgaaaa caatttattt tcagcgatta aagttactaa
    25681 aaatgattat tacattcctt tcagtcggga ttttttttta tttatttttg tactgaatgt
    25741 ttaatgtgat ggaatataaa tagtaaaaac agtgaaaaat cgttttattt cgggatttaa
    25801 attataatat ctatatttgt agtcataaac gagagcctat ctttagagct gttattatca
    25861 tatttatatt tatttgaagg aataaaatgc taaaaaaaga tttacaaccg gaatttgtac
    25921 aaagtttatg cgaagacagc gataaagtac tgcaatttct tgccgatgtg aaatgggaaa
    25981 agggttttgt gtgtcatagc tgtggtcata ctaatttctg cagcggaaaa actcttgcat
    26041 caagacgttg tacgcgctgt aaaaaagaag aatcggcaac tgctcatacg atatttcaca
    26101 actgtaaatt tcctgtaaac aaagcgtttt atattattta cacaatttgt tttgagaaaa
    26161 agaaacattc tgtcaatgaa ttagcggatg aactttgcgt aaatccgatg acctgctgga
    26221 agtttcgaac taaaataaag aaatgtattc agaaacatca ggatacttcg gaagatgaaa
    26281 ttgatgttaa ggaaatact
//
LOCUS       DAOPYB010000024        25359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1943_length_25359_cov_10.206331, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000024 DAOPYB010000000
VERSION     DAOPYB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 25359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25359
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1943_length_25359_cov_10.206331"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKH58_03410"
     CDS             complement(<1..190)
                     /locus_tag="PKH58_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018921261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HNX88105.1"
                     /translation="MRFRLRLHINKQRHGNTLPFNYQYECSAVIYRIMARSDADFAAW
                     LHDNGFQSDRKQFKLFTYS"
     gene            669..1880
                     /locus_tag="PKH58_03415"
     CDS             669..1880
                     /locus_tag="PKH58_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNX88106.1"
                     /translation="MTAKDKFLNLEKNTKTNQIKKEIISYFLNNGKTTISELAKELDL
                     SVPTVTKLINGMSEDGLISDYGKIPTPEGRHPNYYGMNPTSAYFMGIEMTHQHLNMAL
                     MDFNGDVVRTKFGIPYIYENKLELLDELCTRIMEFIDEETPEIRENIFNINLNIAGRV
                     NPESGYSYSSFYFSEEPISSILSRKLYRNVGIDNDTRAMAYGEYMKGIVKGQKNVLFI
                     NYSWGLGIGIIIDGKPYYGKSGFSGEFGHFPAFDNEIICHCGKKGCLETEASGRAIYR
                     EVLERIKNGETSILSDQVKDIQNLKLSDIIKATNQEDTLCIEVVEEVSEKLGRYIAGL
                     INIFNPELVIIGGQVAETDGFVLMPIRSAIRKYSLNLVNKDSQIELSKLQNKAGVYGA
                     CLLGRNRLLAE"
     gene            2448..4094
                     /locus_tag="PKH58_03420"
     CDS             2448..4094
                     /locus_tag="PKH58_03420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNX88107.1"
                     /translation="MIKYTRFLLPAFLLIAFLCPAKVSATAYYFYIQLKDKKNSPYSL
                     TNPSAYLSKRAIDRRAYYFIPVDSTDLPVSPAYQSQITNLGIHIHCRSKWLNGLTVLV
                     PDTSVISQLRSLSFVKYVQYTGITEENKAAGIARKRNKAMNFDYGAAATQINQLNGSI
                     LHDHGYRGENIHIAVLDAGFLNVNQNPAFDSLRMENRLLGTKDFVNPASDIFSEDAHG
                     AYVLSSMTANIPGKYLGTAPKASYLLIRTEAADDEYLCEPDFWVSGIEYADSAGVDLA
                     TTSLGYTQFDDSTMNYTYADMNGKTARASIAATMAFNKGIMLLNSAGNDGNKSWHYIG
                     VPADAEGVIAVGSVSKSGSPSSFSSFGPSYDNRIKPELCAMGSSSSLVYFADSLVVTG
                     SGTSFACPILAGMTACYLQAAKAKMPSHTLSLLRKHLNESGNLYSEPTAQMGYGIPDF
                     AKAYNAINYTSVDNIRNDLRVKLLVNSTENKLKIRIPDYTSSLSNAQLVIYSPSGQTV
                     LQAKLNSWSEDFDISALNTGIYLLTIQENGIKYRTKIAIR"
     gene            4091..5323
                     /gene="xseA"
                     /locus_tag="PKH58_03425"
     CDS             4091..5323
                     /gene="xseA"
                     /locus_tag="PKH58_03425"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNX88108.1"
                     /translation="MNSVSLSELTSHIQGVIRTNFETALWVRAEISELRENNGHCYLE
                     LIEKDAGTDNILAKTRANCWANVYRMLKPYFESSTGESLRAGLNVLVAVTVEFSGLYG
                     FSLNIRDIDPSFTIGELAARRLQIIRQLEADGIADMNKQLPFPVLPQRLAIISSATAA
                     GYGDFGDQLKKHPSSFAFYPKLFPAIMQGEQAEKSIVAALERVYENLSMFDVVVIIRG
                     GGATTDLACFDSYNLALNCAQFPIPVIAGIGHQRDISILDMVAHTSVKTPTAAAEFLI
                     DKMQETENELYNLANDIHFFISQFLREEEQLLERKKLQIRQAASAGLLKRKHLNEQLL
                     LRLKTVIKQSILHQKNRIELIQNRIEVHSPAYLFERGYSITTLNGKRLTSVSDVKPGD
                     LVKTWLSDGKFESEVKES"
     gene            complement(5607..6809)
                     /locus_tag="PKH58_03430"
     CDS             complement(5607..6809)
                     /locus_tag="PKH58_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNX88109.1"
                     /translation="MGKVLIIGAGGVGTVVVHKVAQNADVFTEIMLASRTKSKCDVIA
                     ADVKKRYNVNVKTAQVDADSVPELVKLFNEFQPKLVINVALPYQDLTIMDACLESGVN
                     YLDTANYEPKDEAKFEYSWQWAYQDKFKKAGLTAILGCGFDPGVTSVFTAYAAKHHFD
                     EIHFLDIVDCNAGDHGKAFATNFNPEINIREVTQKGKYFENGKWIETEPHQIHKPLNY
                     PEIGAKESYVIYHEELESLVKNFPTMKRARFWMTFGQEYLTHLRVIQNIGMARIDEVE
                     YNGQKIVPIQFLKAVLPNPGDLGENYKGKTSIGCRIKGFKEGKVKTYYIYNNCSHEDA
                     YKETGTQAVSYTTGVPAMIGAMMFMTGEWSKPGVFNVEQFNPDPFMEQLNKQGLPWKE
                     LQDVDLEL"
     gene            complement(6980..8110)
                     /gene="nspC"
                     /locus_tag="PKH58_03435"
     CDS             complement(6980..8110)
                     /gene="nspC"
                     /locus_tag="PKH58_03435"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNX88110.1"
                     /translation="MNYTEIPSPCYVLDEKAFRKNLALIRSVKERAGVEIILAFKAFA
                     MWSVFPIVREYIPCSTASSLAEARLAFEEMGSPAHTYAPAYSETEFPEIMSYSSHITF
                     NSLTQFDKYYPQTTQKNISCGLRINPEFSDVETDLYNPCAPGSRLGVVTSLLGDKLPR
                     GIEGLHFHTLCESTSYDLEKTLNFVEEKFGKYFPQIKWLNMGGGHLMTREGYDVEHLI
                     ATLRNFKKKYPHLHIILEPGSAFAWRTGVLVSTVLDVVENGGIKTAMLDVSFACHMPD
                     CLEMPYKPVILGACDAEPGKPTYRMGGNSCLSGDFYGSWSFDNELKPGDRVVFDDMIH
                     YTMVKTTMFNGVTHPSIGIWTADDTFKIIRKFGYEDYKNRMS"
     gene            complement(8145..9800)
                     /locus_tag="PKH58_03440"
     CDS             complement(8145..9800)
                     /locus_tag="PKH58_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX88111.1"
                     /translation="MKKLLITIISVSLFVAAHAQSNQNSEYRKFEITKNLSVFNTILR
                     ELDANYVDTLKYDELVKTGINNMLMKLDPYTVYMPEEMNDDLKMMTTGEYAGIGAMIM
                     QKDGKVVISEPYEGKPAQLNDLRAGDEILEVDGVNTKNKTSSDVSELLKGKNGTQITI
                     KIARPGEKKPIVKQFMRQNIQFNPVSYTSIMGKTGYLLLDDFTDKAAQEIKSVITEMV
                     KKHQITSVVIDLRDNGGGLVDEAVKIVGYFVPKGTEVVVTKGKSSQMDKTYRTPSEPV
                     FPDLKLAIIVNRSSASASEILAGAMQDLDRAVIIGERTYGKGLVQSIRPVGYDGHIKI
                     TTAKYYIPSGRCVQALDYSHRNEDGSVGRIPDSLVSTFKTKNGRIVRDGGGILPDTVT
                     IDSRKLNISYYLLMQNMYFDYATEYVLKHEKIAPAATFRLTDQEYSDFMNYVINEKKF
                     TYTTQTGRYYNQLFEVAGYEGLDEEAKVEFEALKAKLVPDVRKNMEKNRKDIEDMLST
                     EILKRYYFQKGQIEYTLRDDHEIRLAVDILGNQEVYTKILSGK"
     gene            complement(9829..10281)
                     /gene="coaD"
                     /locus_tag="PKH58_03445"
     CDS             complement(9829..10281)
                     /gene="coaD"
                     /locus_tag="PKH58_03445"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNX88112.1"
                     /translation="MKRAIFPGTFDPFTIGHYNLVTRGLKLFDEIVIGIGVNQSKKTL
                     FDTEKRIDIIQQAFSSEPRVQVMTYDSLTVDFAVSAEADYILRGVRSVADFDYEHTIA
                     NSNRMLSGIETVVLFTDKEYSFISSTVVRDLIAYGKDITAFLPPHVKL"
     gene            10543..11778
                     /locus_tag="PKH58_03450"
     CDS             10543..11778
                     /locus_tag="PKH58_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88113.1"
                     /translation="MKIFRFSIILCLILTASQSGDACTTFLISGKSTPDGKPILYKNR
                     DTDKLQNALVSFSDGKYKYVGLVDCSETSKNMVWGGYNETGFAIINSAAYNNNIGDST
                     KLIDREGVVMKLALQNCRTLEDFEQLLLSLPKPLGVDANFGVIDAAGGAAYYETGNFR
                     FIKYDANDPAVAPDGILIRTNYSHSADPAKGYGFCRYNTAKIALNKAFAEKDLSPQHL
                     FNAVSRNLTHSLTGTDLTTDLPSNKDIPSYKFFTDYIPRFSTASVVMIIGAQDQTHAG
                     ETMMWTILGFPLTSVAVPVWISADNRLPASVKMGENQQSPLCNFALKLKENCFPLHYG
                     NGKNYINLAVVVNKQNTGYLQQLQPVENEIFSKAEKLRSEFEKGKKSDKMITEFYDWL
                     DKYLQKNYKELFNLSLFEE"
     gene            11928..13340
                     /locus_tag="PKH58_03455"
     CDS             11928..13340
                     /locus_tag="PKH58_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX88114.1"
                     /translation="MQEHLKDKIFKLIGETADEMQLECYVIGGFVRDIFLHRPSKDID
                     VVVVGNGIELAERLAKKLGKKAKFAVFKTYGTAQVKTPEWEVEFVGARKESYSHDSRN
                     PVVETGTLEDDQNRRDFTINALALCLNKDRFGELVDPFNGLQDLQNFVIRTPLNPDIT
                     FSDDPLRMMRGIRFSAQLRFFLETNTFDAIVRNRNRISIITKERIADELNKIMLSPKP
                     SVGFVLLEKTGLLELIFPELLALKGAETKDGIGHKDNFYHTLAVLDELATHTDNLWLR
                     WAALLHDIGKPRTKKFDRKLGWTFYNHNFIGEKMIPEIFRKMKLPTNEKMKYVQKMVS
                     LHMRPIVLSEDEVTDSAVRRLLFDAGDDIEDLMMLCEADITSKNQEKVKRYRQNFMLV
                     RQKLKDIEEKDRIRNFQPPVSGQEIMDVFGLPACATVGDIKIKIKDAILDGIIPNEYN
                     AAYEYMLNIGKELNLIEKKP"
     gene            13384..15435
                     /locus_tag="PKH58_03460"
     CDS             13384..15435
                     /locus_tag="PKH58_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88115.1"
                     /translation="MGEKRIIVLILAVAVYFISAPAAPKQVYITVDNSGSMIGYKYMV
                     SAYTAQLITLLNAGNDVTVIMNGVARKIPDTPKNIKSLRIAQPNINREWGSSLFGSQI
                     GDIASFNELFKKNPGRDQWLFVIGDGNWNTNRFPTVTANFLNLVMENHLRIFFVPYGS
                     NEYYPGDFTSVIRKVRNARVLNGATSFSNVFNNCVVLIYYLTSTECKLPATNLVDEQT
                     VEVNSVVPAKKYLIFYQDNRDENELPEITATILDTVTMKAELLGKPSTFGMQIKNYPN
                     LLSSALWKVENKSRIQSGKPLILKFDRKIEQNKLKVFVFPDKSFNMKSDSIIQISSKS
                     DKTEKSGTSSGNIIVVNKNELTKALEKTAYNTKSVESSKGNMYANDRFRSKIQPLKPK
                     SLVIPSPPLTPVTFRKIAEDEPRIGYLLQIRRNSSFDPEKHEIAFDPGLKLYFKDIST
                     WSDSEKTVFLLEPRNQLISLLMPDTLELNLKINSKVSENTCFADNDRIIIRTATAKGK
                     VPVTELIIRLFSGILFIIYLILLSCKARFKKGAIISYRSVDESELCINITLRKKGITN
                     WLNRWLNPFIPENSEIVFAKMDAKILFTACRKKTAVRIHKSFLDNRHLYLDHHARKVK
                     NYAYITEDNKLIIKTNNSYTGKPAVITYNRMKNVRDDVPVFRFLLFLSGIILMAVLVL
                     I"
     gene            complement(15519..15595)
                     /locus_tag="PKH58_03465"
     tRNA            complement(15519..15595)
                     /locus_tag="PKH58_03465"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15559..15561),aa:Asp,seq:gtc)
     gene            15875..17638
                     /locus_tag="PKH58_03470"
     CDS             15875..17638
                     /locus_tag="PKH58_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNX88116.1"
                     /translation="MEITRIFDILDRYLEKFPNQDAALACKRDGQWQKFSIQKYTELT
                     NLMSFGMLEMGIKKGDKVGIVSGNRPEWNMLDFAVMQVGAISVPIYPTISQEDYRHIL
                     NHAEVKMIFIEGKDLRSRLEPILETLSSLKEIFTFNEENNQYKKLDELIELGRQHPHN
                     DELTKIKASIESNELATIIYTSGTTGVQKGVMLSHSNLVNQLLNLKDTPMKWSNRALS
                     FLPLCHAYERILVYLYQYLGMSVYYAESLATIAENIKEINPTMMTCVPRLLEKIYDKL
                     HSAGRDLSFPKKNIYYWAFDLATKYQLEGMSKAMLYKLKVADKLIYSKWREAIGGNFD
                     IVVSGGSAIQPHMASFFSAIGMPVFEGYGMSEGSPVIAVSQRGVNGRKFGTVGPPLPG
                     VEIRIIDRDEIVFRGHNVMMGYYKDPEMTAQVIDKDGWMHTGDTGKFTPEGQLVITGR
                     LKSIFKTSFGKYVNPQTVESKFIESGFIENLIVLGENKKFAAALIVPDFTYLKVWCKE
                     HKIPYTTNEEMIKHPDVIKRFQKEIKHYNHFFGDYEQIKRYQFVSEEWSQASGFLSPT
                     LKIKRNVIENFYADKIAKLFD"
     gene            17882..18700
                     /locus_tag="PKH58_03475"
     CDS             17882..18700
                     /locus_tag="PKH58_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNX88117.1"
                     /translation="MVKQKKNSKILFISQEIFPYLPESEIANQGRYLPQAAQERGKET
                     RTFMPKFGLVNERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQSARMQVYFIDNDD
                     YFQRKNTIKDDDGVEYDDNYERTIFFVRGVMETVKKLRWTPDVIHCQGWMSALAPLYI
                     KKAYNEDPFFKHSKVVYSIFNDDFIKPFHAQFADNLLLEGIHKKDLASISGEEIDYVA
                     LTKLAIDYSDGVIQSSKSINQHLTEYVAEKKIKFLPYVDIEDYADDYLNFYDSL"
     gene            18741..20129
                     /locus_tag="PKH58_03480"
     CDS             18741..20129
                     /locus_tag="PKH58_03480"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HNX88118.1"
                     /translation="MKFKFVFSLLILASVFFASCDDSALNLGENIQPTSDKISIKADT
                     FHLTTETYMVDYIYSTPDSFLLGTYIDNVLGTTRADILTQFYYPTDYKFLDKSVAVAT
                     PDSVKLYMTFDSYFGLRTSPMEISVYELQDSLRMKENYRSDINPANFVNFGKRLNAEN
                     EGIFTVYDVKDDADNTSVSVKLNNDFLNRFFTSDPAVFASDRNFLNFFKGLYITTDFG
                     STTLLNVSGVYLMLYYHNTYSKDGSTSKVTLTFPASNLEVKRVNRITHPSRSANFTQN
                     DGINYISSPANCYTRVKIPIEKMRSRLTLPANKHRIINSAILQVNVVDKDTIGTRLPY
                     VKNMLLVKEDELDDFFKNNKLPTSGSSYLVSLDSTSLTSTTYEYHYTFNGLSTLIDAE
                     LKNPAESHKDGYVNMVLVPVDVNKTTTSSYYSSSTTINSVRQNTQLQAVGVYSGKNKK
                     IPMKMEVVYSGF"
     gene            complement(20220..21533)
                     /gene="der"
                     /locus_tag="PKH58_03485"
     CDS             complement(20220..21533)
                     /gene="der"
                     /locus_tag="PKH58_03485"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNX88119.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRLTNSRRAIVNEEAGTTRDRQYGK
                     SEWCGREFSVIDTGGWVVNSTDVFEEEIKRHVLLAIEESDVILFLVDVQNGVTDLDIH
                     VAQILRRADKPVILVSNKTDTFEMQYQAAEFYKLGLGDPFILSAINGLGTGELLDELM
                     KHFKPDVSEEIELELPRFAVVGRPNVGKSSMINAFIGEDRTIVTDIAGTTRDSIYSRF
                     NKFGHDFYLVDTAGIRKKAKVNEDLEYYSVVRSIRAIENSDVCILMLDATRGLESQDL
                     NIFSLIQKNKKGLVVCVNKWDLIDNKEANDIKKIEKSIRERIAPFVDFPIIFTSVLTK
                     QRILKVLDTAVRVFENKQRKVPTAKLNELLLPLIENYPPPALKGKYIKIKYVTQLPGT
                     YIPTFVFFANLPQYVKEPYRRFLENKIRANWDFHGTPINVFIRQK"
     gene            complement(21793..25008)
                     /locus_tag="PKH58_03490"
     CDS             complement(21793..25008)
                     /locus_tag="PKH58_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX88120.1"
                     /translation="MKKRSFILLMGIFITFGVFAQLQENRYDEITNPKLTCINKLPAH
                     SSFFSFSNVAEAQKALYSSKGSDFISLDGTWKFNYAEDFSKRPMNDFYNENFDASSWK
                     DIKVPGNWEVQGFGYPIYVNTTYEFTSPGHEPFWNRPNPPYVPKEFNPTGTYRREFEI
                     PQSWIGKEIILSADAAKGAAYYYLNGEFIGMNKAGKLPARFDITAKVKAGKNVLAVQI
                     HRFSDANYLECQDFWRLSGFERDVYVYARPKVHIADFFAHTPLDATYTNGQFSLEVAL
                     ENSAITSDELAVTYTLKDATGKMTASETKTINVKDKVQVKFLKEIPDVKKWSAEEPNL
                     YALSIELKDKNGNTLEATAVKVGFRTSEIKNKQFMINGQPVLVKGVNVHEHNEFTGHY
                     VSEELMRKDFELFRKYNVNTVRTCHYPQQELFYRLADEYGIYVIDEANIEAHGMGYDL
                     RKGATLANNPLFLEAHLARTSEMIERDKNHACVVTWSLGNESGNGYNMYATYNLIKGR
                     DTSRPVQYERAGLEWNTDIFCPMYDRPEEIESYAKNQNSDRPLILCEYAHAMGNSLGN
                     FIDYWNIIRSNPLLQGGCIWDWVDQGIAQKDAEGNKFWAFGGDFGPKGTPSSGDFCCN
                     GLILPDRTTKPHTEEMRKVYQNIWFKKFDAQKGTVDVYNENFFIDLSQYSFHYTIKSN
                     GKVLKTGQIALNVKPHDTKTVVIPDISKYFKVKNQVTINFEARQKTDTRYIPAGWVVA
                     RDQFIVNDYPKMVLNAKSPATVTQNTANLIFNGTDFSVIFDKESGVMTSYKYKGTEYI
                     SDHFGLKPFFWRASTDNEYGAGLPKKLNNWKKASYTELKAENLNVKTGENTIVSCDYR
                     FPQTNTSWKVIYTIYKNGSIHVENAVDATKCDFPLIPRIGMRMQLPGEFVNAEYYGRG
                     PWANYEDRKTSTFVDRYKSSIIDMVDKYVMTQENAHHTDAAWLAVTLGSGKGMAFFAD
                     DKFQFNVSNYLLETIANAEDWNNDAAVGTAPKIKHINEYKPSDKVDLFIDYRMMGVGG
                     NNSWGEWPMLPYRIIPKETNISYGFTLVPIDNINAVDKMLK"
BASE COUNT         7556 a         5293 c         5109 g         7401 t
ORIGIN      
        1 gggaataagt gaaaagtttg aattgtttcc tgtcggactg aaatccgtta tcgtgcagcc
       61 aggcagcaaa atcggcatcc gatcgcgcca taatcctgta tattactgca gagcattcgt
      121 actggtagtt gaaaggaagc gtattcccat gcctttgttt gttgatatgc aatcgtagcc
      181 tgaacctcat aagcaactaa tatatgttga tttaaaaata tctccgtaaa tctaaaaatc
      241 gccaataaat ctttcaaccg taaaaataga gatttttatg aacttgttat gttttttaca
      301 ttaatatttg tttaatttct ttatttgact atgtttgaat agaaaagaat aatattagcg
      361 ttcaaatttc aaccattcaa aaaagtattt ggtaaatgtg atttttcaga aatcaggctg
      421 aggaactttt caagcctgca ttttgcaaag atagaagggg tttttgacag tttatgacaa
      481 aaaaaaaacc agatacatat attgttttga aaaaaataca atcaggattt caagctaact
      541 tttttatatt tcttaaaata cttttaaata tttatattat ttactatgtt tgcaagatat
      601 aaaattcagc tatctttgtt ttaaattgaa aattcgatta tttataaaaa cctgataatt
      661 caaaatatat gacggcaaaa gataagtttt taaatctgga aaaaaacaca aaaaccaacc
      721 agatcaaaaa agaaattatc agttattttc tgaataacgg caaaacaact atttctgaac
      781 ttgccaaaga acttgattta agtgttccga ctgtcaccaa acttattaac ggcatgagtg
      841 aagacgggct tatatctgac tacggtaaaa tcccgacacc tgaaggcaga caccccaatt
      901 attacggaat gaatcccact tcagcatatt ttatgggcat tgaaatgacc caccaacatt
      961 taaatatggc tctgatggat tttaacggcg atgtggtcag gacaaaattt ggcataccat
     1021 atatctatga aaataaactg gaattgctgg acgagctatg tacaagaatt atggagttta
     1081 ttgacgaaga aaccccggaa atcagagaga atatctttaa catcaacctg aatatagccg
     1141 gcagagtgaa tccggaatca ggatacagtt acagcagttt ttacttcagt gaagagccta
     1201 tcagcagcat actttcgagg aaactctatc gcaatgttgg cattgacaac gacacccgtg
     1261 caatggctta cggcgaatac atgaaaggaa tagtaaaagg acaaaaaaac gtacttttca
     1321 tcaattacag ttggggcttg ggtattggta tcatcattga cggaaaacct tactatggaa
     1381 aatcaggatt ttcaggtgaa tttggtcatt ttccggcttt cgataatgaa atcatctgcc
     1441 attgcggaaa aaaaggctgc cttgaaactg aagcatcagg acgtgcaata tacagggaag
     1501 tgcttgaaag aatcaaaaac ggagaaacct ccattctttc cgaccaggtc aaagacatcc
     1561 agaacctgaa actatcggat atcataaaag ccaccaatca ggaagacacc ctgtgcattg
     1621 aagtagtgga ggaagtgagc gaaaaattag gtagatacat agcaggactt atcaatatct
     1681 tcaacccgga acttgtgata atcggtggtc aggttgccga aacagacggc ttcgtattga
     1741 tgccaatccg tagcgcaatc agaaaatact cactaaatct tgtaaacaaa gactctcaga
     1801 ttgaactttc aaaattacaa aacaaagcag gcgtctatgg agcctgttta ttaggaagaa
     1861 acagattact tgcagaataa ctgcgtttga attaaatact ctcactaacc taccagtttt
     1921 tattggatta agattttaaa ttattgttgt cagacaaaaa gtacaaatac aactgtaatt
     1981 tgctgtaatc ataacgaata agaatattaa tccccgccgg gacttacttt ccggtagtaa
     2041 ccaaatcgca caaatatttt gcaacgcttt tgcaaaaaca tccacagaat agatgaatct
     2101 ttatagtagg aagtgaaaaa taagatttct tgccacagat ggaacattcc gatccatcgg
     2161 aagtggcaaa atattaaggt taatcggaca atagtgatcc acaaaataaa aaacttataa
     2221 atttacgaag ctgacgactt aaatatcaac ggctgaaatc ctgcaactta cgcggtcatt
     2281 agtcagaata aaaaagccac caatagtagc ttttaatttt cattgtttgt gaaaattgat
     2341 ttattgagat ttttattaaa agaaaaatat atcaattcct tgattaaaat tgcttatttt
     2401 tgtgaattgt tcgcgacgaa gttcctttta actttaatct caggaatatg ataaaatata
     2461 cccgttttct gttacctgcg tttttactga tagcatttct ttgtcctgct aaagtcagtg
     2521 ccacagccta ctatttttat attcagctga aagataagaa aaattccccc tactccctta
     2581 ccaacccgtc ggcttatttg tcgaagcgtg ccattgaccg gcgtgcctac tattttattc
     2641 cggttgacag taccgatttg cctgtcagtc cggcctatca gtctcaaata accaatctgg
     2701 gaatacatat ccattgccgc tccaaatggc tcaatggtct gacagtactt gtaccagaca
     2761 ccagcgttat ttcacaactc agatcgctgt cattcgtcaa atatgtgcaa tacaccggca
     2821 ttaccgaaga gaataaagct gcgggcatag ccaggaaaag aaataaggcc atgaattttg
     2881 attatggcgc tgcagccacg caaattaatc agttgaatgg cagcatcctg cacgatcatg
     2941 gttaccgggg tgaaaatatc catatcgccg tgctcgatgc tggcttcctg aatgtgaacc
     3001 aaaacccggc cttcgacagt ctgcgtatgg aaaaccgttt gctgggaaca aaagattttg
     3061 tcaatccggc atccgatatc ttttcagaag atgctcatgg ggcatatgta ctttcgtcca
     3121 tgactgccaa cattccgggg aaataccttg gaacagcccc gaaagcatcc tatctgctga
     3181 tacgtaccga ggctgctgat gatgaatatc tctgtgaacc tgatttttgg gtatcaggca
     3241 ttgaatatgc cgacagcgcg ggagtggatc ttgccaccac ttcgctcggg tacacacagt
     3301 tcgacgacag cacgatgaat tacacctatg ccgatatgaa tggaaaaaca gcgcgggcaa
     3361 gtattgctgc cacaatggct ttcaacaaag gtattatgtt gcttaacagc gccggcaatg
     3421 acgggaataa atcctggcat tacattgggg taccagctga tgctgaaggt gtcattgctg
     3481 taggttcggt gtcaaaatcc ggatcaccca gcagcttctc atcgttcggt cccagctacg
     3541 acaatcgtat taaacccgaa ctgtgcgcca tgggtagttc atcctcattg gtttactttg
     3601 ccgacagcct ggtagttacc ggcagcggca cttcgtttgc ctgccccata ttagccggaa
     3661 tgactgcctg ttatctgcag gcagccaaag caaaaatgcc ctcgcatacc ctgtcattgc
     3721 tccggaaaca tttgaacgaa agcggaaacc tctacagcga accaactgct cagatggggt
     3781 atggtattcc cgattttgca aaagcttaca atgcgataaa ctatacttct gttgataata
     3841 tcaggaatga tttgcgtgta aaactgcttg tcaactcaac cgaaaacaaa ctgaaaatca
     3901 gaattcctga ctatacaagc agcctgagca atgcacagct cgttatttat tcgccgtcag
     3961 gacagactgt acttcaggcg aaactcaact cctggtcgga ggattttgat atatccgcat
     4021 taaatactgg catatatctg cttaccattc aggaaaatgg aataaaatac aggacaaaaa
     4081 ttgcgataag atgaattcag tctccctctc agaattaacc agccatatac agggagtaat
     4141 ccgtaccaat tttgaaacgg ctctgtgggt tcgtgctgaa atcagcgagc tgcgcgaaaa
     4201 caacgggcat tgctacctgg aactgattga aaaggatgcc ggtaccgata atattctggc
     4261 aaaaacacgg gcaaattgct gggcaaacgt ttaccggatg ctgaaacctt acttcgaaag
     4321 cagcacggga gaaagcctga gagccggact gaatgtactg gtagctgtta ctgtcgaatt
     4381 tagcggatta tatggtttca gcctgaatat ccgtgatatt gacccatcgt ttaccattgg
     4441 cgaactggct gcgcgccgat tgcagattat ccgtcaactg gaagccgacg gaatagccga
     4501 catgaacaaa cagctgcctt tccctgttct gccccaacga ctggccataa tttcatcggc
     4561 tacagctgcc ggttacggtg atttcggcga ccagttgaaa aaacatccgt cctcattcgc
     4621 cttttacccg aaacttttcc ccgcaattat gcagggcgaa caggctgaaa agtcgattgt
     4681 ggctgctctg gaaagagttt atgaaaacct ttcgatgttt gatgtggtgg taatcattcg
     4741 cggaggtggt gctactaccg atctggcttg tttcgattcg tacaacctgg cgctcaactg
     4801 cgcacaattt cctattccgg tgattgccgg cattggtcat caacgcgaca tcagcattct
     4861 ggacatggtg gcacacacca gcgtaaaaac cccgactgca gctgccgaat ttctgattga
     4921 taaaatgcag gagacagaaa atgaacttta caatctggct aatgatattc atttctttat
     4981 cagtcagttc ctgcgtgaag aggagcaact actggaacga aaaaaactgc agatccgtca
     5041 ggctgcctca gcaggtttac tgaaacgaaa acatctgaac gaacaactgt tgttgcgtct
     5101 gaaaactgtc atcaaacagt ccatccttca tcaaaagaac aggattgaac tcattcaaaa
     5161 caggattgag gtacattctc cggcatattt atttgagcgg ggatacagta tcactacgct
     5221 aaacgggaaa aggctaactt cggtcagtga tgtaaaaccc ggcgatcttg taaaaacgtg
     5281 gctttcggat ggaaaattcg agagtgaagt aaaagagagt tgagaatgat taatgagcca
     5341 atgtgtaaat ttgaaaatgt gcaaatttga aaattcatag catgtgatta gaaattaata
     5401 gttattggtt attgagttat taagttattg gcttaaatat cctctccacc ttaaagtccc
     5461 ggttcttttt atcttacttt caaagtttcc aggtcccggg tctattagtc caaaagttcc
     5521 ggttctacaa gtccctgttc actaacattc cgtatatcaa ataaaaaaag acactcatgc
     5581 atgtcgctga atgtcttttt tgtatgtcag agttcgagat ctacatcctg aagctctttc
     5641 cagggaagac cctgtttgtt gagttgctcc atgaacggat cgggattgaa ttgttcaaca
     5701 ttgaatacac cgggtttact ccattcaccg gtcataaaca tcatggcacc aatcattgcc
     5761 ggtacaccgg tagtatagct gacagcctgc gtgccggttt ctttgtaggc atcttcgtga
     5821 ctgcaattat tgtaaatata ataggtttta acttttcctt ctttaaaacc ctttatgcgg
     5881 catccgatgg aagttttccc tttgtagttt tcgcccagat caccgggatt gggcaaaaca
     5941 gccttgagga actgtatcgg aacaattttt tgtccattgt attccacctc gtcaatacgt
     6001 gccataccga tattctgaat aacccgcaag tgagtaagat attcctgtcc gaatgtcatc
     6061 cagaaacgag cacgtttcat ggtcgggaaa tttttaacca gtgactcaag ttcttcgtga
     6121 taaatcacat acgattcttt tgctccgatt tcgggataat tgagtggttt atgtatctga
     6181 tgaggttcgg tttcgatcca tttaccattt tcaaagtatt ttcctttctg ggttacctcg
     6241 cggatattta tttcgggatt aaagttggta gcaaatgctt tgccgtgatc accggcattg
     6301 caatcgacaa tatccagaaa atgaatttcg tcgaaatgat gcttggctgc ataagcggta
     6361 aatacactgg ttacacccgg gtcgaagcca catccgagta tggctgtcag acctgccttt
     6421 ttaaatttat cctgataagc ccattgccag gaatattcaa attttgcttc gtctttcggc
     6481 tcgtaattgg ctgtatcgag ataattcact ccgctttcca gacatgcatc cataattgtg
     6541 aggtcctgat aagggagcgc tacgttgatg accagtttcg gctggaattc gttgaagagt
     6601 tttaccagtt ccggaacact atctgcatcg acctgagctg ttttgacatt tacattgtac
     6661 cgttttttaa cgtcggcagc aattacatca cattttgatt tggtgcggct tgccagcatg
     6721 atttctgtaa acacatcagc attttgagcc actttatgaa ctactacagt tcctacgcca
     6781 ccagcgccaa taatcaatac ttttcccatt ttagtcagta ataaatttat agataaaatt
     6841 tcactcttaa tgttggtcaa aaaaatgttc tgttataata aattttgaga tacaaatgta
     6901 cgtcaatatc tcaaacgttg caaattaatt tgtttcagtt ttattaaaaa aattaattga
     6961 ctttacaaac ttatcttaat caggacattc ggtttttata atcctcataa ccgaattttc
     7021 tgatgatttt gaaggtatca tcagctgtcc atatgccgat agaagggtgt gttactccgt
     7081 tgaacatcgt tgttttcacc atggtataat gtatcatgtc gtcaaatacg acgcggtcgc
     7141 cgggcttcaa ttcattatca aacgaccagg acccgtagaa atcgcccgac aggcaactgt
     7201 tgcctcccat gcggtacgtc ggttttcctg gttccgcatc gcaggcgcca agaatgacgg
     7261 gtttataggg catttcaagg caatcgggca tatggcaggc aaacgacaca tcgagcatgg
     7321 cagttttaat tcctccgttt tccactacat cgagtaccgt tgaaaccaga acacctgttc
     7381 tccaggcaaa agcacttccg ggttcaagta taatgtgcag gtgcgggtat ttctttttga
     7441 aattgcggag agtagctatc agatgttcca catcataacc ttcacgggtc atcagatgtc
     7501 ccccgcccat attcaaccat tttatttgcg ggaaatattt tccgaatttc tcttccacaa
     7561 agttcagtgt tttctccaga tcgtaagaag tggattcgca cagcgtgtga aaatgtagtc
     7621 cttctatccc ccggggtaat ttatcaccaa gaagtgaagt caccacaccc agacgcgaac
     7681 cgggagcaca gggattgtac aaatcggttt ccacatccga aaattcagga ttgatgcgaa
     7741 gaccgcagga tatatttttt tgtgtggtct gcggataata tttatcaaat tgtgtcaatg
     7801 agttaaacgt gatgtgactg ctgtaactca ttatttcggg aaactcagtt tcggaataag
     7861 ccggtgcata agtatgcgcc ggacttccca tttcttcgaa agccagacgg gcttcggcca
     7921 gcgaacttgc cgttgagcag ggaatatatt cccgcacaat ggggaaaacc gaccacatgg
     7981 caaatgcttt aaaggcaaga ataatctcaa ctccggcacg ttcttttacc gatcttatca
     8041 gcgccagatt tttccggaag gctttctcgt caagcacata acaaggtgaa ggtatttcgg
     8101 tataattcat attttcagaa aactgatttt gatttactaa attactattt tccggacagg
     8161 atttttgtgt acacttcctg gttgccgaga atatcgactg cgagtctgat ttcgtggtca
     8221 tcgcggagtg tgtattcaat ctgtcctttt tggaaataat atcttttcag aatttcagtg
     8281 ctcagcatat cttcaatgtc cttacggttt ttttccatgt ttttcctgac atcaggaacc
     8341 agtttggctt tgagtgcttc gaattctacc tttgcctcct cgtccagtcc ttcatagcct
     8401 gcaacttcaa acagttggtt gtaatacctg cctgtctgag tggtataggt gaatttcttc
     8461 tcattgataa catagttcat aaaatcactg tattcctgat cggtaaggcg gaatgtagct
     8521 gccggtgcta ttttttcgtg ctttagcaca tactcagtag cataatcgaa atacatattt
     8581 tgcatcagca ggtaatacga gatgttcagt ttacgtgaat caatagtaac cgtatcgggc
     8641 agaatacctc ccccatcgcg gacaattcgt ccgttttttg ttttgaatgt tgaaaccaga
     8701 ctgtctggaa ttcgtcctac acttccatcc tcgttgcggt ggctgtaatc aagtgcctgc
     8761 acacagcgtc cactgggtat gtagtatttt gctgtagtaa ttttgatatg cccgtcgtag
     8821 cctaccggtc tgatactctg aacaagtcct tttccgtagg tccgttcacc gataatcaca
     8881 gcacggtcaa gatcctgcat ggctcctgcc agaatttcgg aagcggaagc tgaggaacgg
     8941 tttacaatga tagccagttt caggtcaggg aaaacaggct cggagggtgt gcgatacgtt
     9001 ttgtccatct ggctcgattt tccttttgta acaacaactt ctgtcccctt tggaacaaag
     9061 taaccaacaa ttttcaccgc ttcatctacc aaaccaccgc cattgtcgcg caaatctata
     9121 acgacagagg ttatctgatg tttttttacc atttcagtaa taaccgattt aatctcttga
     9181 gcagctttgt cggtaaaatc atcgagcagc aaatatcctg tttttcccat aatactggtg
     9241 tagctgacgg gattaaactg aatgttctga cgcataaact gctttacgat tggttttttc
     9301 tcacccggac gtgcaatttt aattgtaatc tgcgtcccgt ttttaccttt caacagttca
     9361 ctgacatccg aactggtttt atttttggta ttcacaccat ccacttccag tatttcgtca
     9421 cctgcgcgta ggtcgttcag ctgcgctggt tttccctcgt atggctctga aatcacaact
     9481 tttccgtctt tctgcatgat catagcccct attccggcat attctccggt agtcatcatt
     9541 ttcagatcgt cattcatctc ttcgggcata taaacggtgt acggatcaag tttcatcagc
     9601 atgttgttaa tgccggtctt aaccagttca tcgtatttca gggtatccac gtagttggca
     9661 tccagttcgc gcaatatcgt attgaataca ctcagatttt tagtgatttc aaatttccgg
     9721 tattcggaat tctgattact ttgagcgtgt gctgcaacaa ataaacttac tgaaatgatg
     9781 gtgatcaata actttttcat atcggaaata taagggaagt gattattatt aaagtttaac
     9841 gtgagggggc aaaaatgcgg ttatatcttt tccgtaagca atcagatccc tgaccaccgt
     9901 ggatgaaata aatgagtatt ctttatcggt aaataaaacg accgtttcaa ttccgctcaa
     9961 catacggttg gaattggcaa tggtatgttc gtagtcaaag tctgccacag aacgcactcc
    10021 acgcaggata taatcagcct ctgctgacac ggcaaagtca accgtcagac tgtcgtaggt
    10081 cattacctgc acacgcggtt cgctgctgaa tgcctgctga atgatatcta ttcttttttc
    10141 ggtatcaaat agtgtttttt tggactggtt tacgccaatg cctatgacaa tctcatcaaa
    10201 caacttcaaa cctctcgtta ccaggttata atgaccaatg gtaaaaggat cgaatgtacc
    10261 ggggaatatt gctcgcttca ttttcttatc ttaatatctt tttttataac agggtgtcgt
    10321 actgtaacaa cctgacaaat agtaaaaaaa atacctgaaa tattctaaga tataaattaa
    10381 caagtggcaa agatagatat tttttatttc ttaaagtttt aataaatgtt gtttctgcat
    10441 cccgaggaga cttaactccc ggtcactgta tttccgtaaa catttttata tatttgtagg
    10501 acataacgag taaacaatta atcagcattt ataaaacaca ttatgaaaat attcagattt
    10561 tcgataattc tttgcctgat acttacagcc agtcagtccg gtgatgcatg caccactttt
    10621 ctgatttcgg gaaaatccac tcccgacggg aaaccgatct tatataaaaa ccgggacacc
    10681 gacaaactgc aaaacgcact ggtatcattt tccgatggga aatacaagta tgttggtctg
    10741 gtggattgtt ccgaaaccag taagaacatg gtttggggag gatataatga aaccggattt
    10801 gccatcatca actcggcagc ttataacaac aatatcggag acagcacgaa actcatcgac
    10861 cgggaaggtg tggtgatgaa actggcgctg caaaactgcc ggacactgga ggattttgag
    10921 caactcctcc tttcacttcc aaaacccctt ggagtggatg ctaactttgg tgttatcgat
    10981 gcagccggag gtgctgctta ctacgaaacc ggaaattttc gatttataaa atatgacgcc
    11041 aacgatccgg ctgtagcacc tgacggcata cttatccgta ccaactattc gcacagtgcc
    11101 gacccggcta aaggttacgg cttttgcaga tacaatacag caaaaattgc cctgaacaaa
    11161 gcctttgctg agaaagatct gagtccgcaa catttattta acgcagtttc ccgcaatctc
    11221 actcactcgc tcaccgggac cgatttaaca accgatttac cttcaaataa agatattcct
    11281 tcatataaat tttttaccga ttatattccc cgattcagca ctgcgtcggt ggtgatgata
    11341 atcggtgcgc aggatcagac acatgccggt gaaacgatga tgtggacgat actgggtttt
    11401 cctttgactt ctgtagcagt tcctgtgtgg atttcggcag ataaccgcct gccagcatcg
    11461 gtgaagatgg gtgaaaatca acagtcgccg ctttgcaatt ttgccctgaa actgaaagaa
    11521 aattgttttc cgctgcatta cggtaacggg aaaaactaca tcaacctggc cgttgtggtt
    11581 aacaagcaaa acaccggtta tctgcaacaa ctccagcctg ttgagaatga aatattttca
    11641 aaagcagaga aacttagatc cgaatttgag aaaggaaaga aatccgacaa gatgattaca
    11701 gaattctacg actggctgga caaatacctt cagaaaaatt acaaggagtt gttcaacctg
    11761 agtctgtttg aggagtaaaa aaagtaacga aactcagtat aagtaaatgc agcatttttt
    11821 tgttcattat tttataaaga aatcccggtg gctcgttgaa ctcaaatccc gtattttgtc
    11881 cgtttaaatt tattggacta cttttgcagt agaaacacaa aaaatgtatg caggagcatt
    11941 taaaggataa aattttcaaa ctgataggag aaactgccga cgagatgcag cttgaatgtt
    12001 acgtcatcgg cggatttgta cgggatattt ttctccaccg gccgtcgaag gacattgacg
    12061 ttgtggtcgt tggcaatggt atcgaactag ctgaacggct agcaaaaaaa ctgggaaaga
    12121 aagccaaatt cgcggtattc aagacttacg gaacagcaca ggtaaaaact cccgaatggg
    12181 aagtggagtt tgtaggcgcc cgcaaagaat cctattcaca tgactccaga aatccggtag
    12241 tagagacagg cactctggag gacgaccaga accgccgcga cttcaccatt aatgcactgg
    12301 ctctctgcct caacaaagat cgtttcggtg agctggtcga tcccttcaac ggcctgcagg
    12361 acctgcagaa ttttgtcatt cgaacgccat taaaccccga catcactttt tcggacgatc
    12421 cgttgcgcat gatgcgtggc atccggtttt cagcacaact tcgtttcttc ctcgagacaa
    12481 acacttttga tgccattgtc cgaaaccgaa accgaattag catcatcacc aaagagcgta
    12541 ttgccgacga gctgaataaa ataatgctct cacccaaacc atcggtgggt tttgtcctgc
    12601 tcgaaaaaac aggactcctc gaactcatct tcccggaact gcttgcactg aaaggagccg
    12661 agacaaaaga cggaatcgga cataaggaca atttctacca tacactggct gtcctcgacg
    12721 aacttgccac ccacaccgac aacctttggt tgcgctgggc tgccctgctg catgatatcg
    12781 ggaaaccacg taccaagaaa ttcgacagaa aactcggatg gacattctac aatcacaact
    12841 ttatcggtga aaaaatgata ccggaaatct tccggaaaat gaaactgcca accaacgaaa
    12901 aaatgaaata cgtccagaaa atggtttcgc ttcatatgcg tcccattgtt ctgagcgaag
    12961 atgaagtgac cgattcggca gtaaggaggc ttctgtttga tgccggtgat gacatcgaag
    13021 atctaatgat gctctgcgaa gctgatatta cctccaaaaa tcaggagaaa gtaaaacgct
    13081 accgacagaa ttttatgctg gtacgtcaga aactgaaaga tatcgaagaa aaagaccgta
    13141 tccgtaattt tcagcccccg gtaagcggac aggaaatcat ggacgtattc gggttgcctg
    13201 catgtgcaac cgtcggagat attaaaataa aaattaaaga tgccatactg gatggtatta
    13261 ttccgaacga gtacaatgct gcttacgaat acatgctcaa tatcggtaaa gaactgaacc
    13321 tgatcgaaaa aaaaccttag caacgggtaa atttgctata atcaaaagcc aactgattgt
    13381 cacatggggg aaaaacgaat aattgttctg attcttgccg ttgcagtcta ttttatctct
    13441 gcaccggctg caccgaaaca ggtttacatt actgtggaca attcgggcag catgatcggt
    13501 tacaaataca tggtttcggc atacacggct caacttatta cgctgctcaa tgccggtaac
    13561 gatgtcacgg taataatgaa tggcgttgcc cgaaaaatcc ccgatactcc gaaaaacatc
    13621 aaatcactgc gtatagccca gccaaacatc aatcgggaat ggggaagctc attattcgga
    13681 agtcaaatag gtgacatcgc ttccttcaac gaacttttca agaaaaatcc gggcagggat
    13741 cagtggctgt ttgtaatcgg tgacggcaac tggaatacaa accgttttcc gactgttaca
    13801 gcaaatttcc tgaatttggt tatggaaaat catctcagga ttttcttcgt tccctatggc
    13861 agcaacgaat attaccccgg cgatttcacc agtgtcatca gaaaggttag aaatgcccgt
    13921 gtcctgaatg gagccacttc gttcagtaat gtgtttaaca attgcgttgt attgatttat
    13981 tacctcacct caacagaatg taaattaccg gcaacgaacc ttgtcgatga acaaacagta
    14041 gaagtcaatt ctgtggttcc ggctaaaaag tacctgattt tctatcagga taaccgggat
    14101 gaaaatgaac tgcccgaaat tactgcaacc atcctggata ctgtaaccat gaaagctgaa
    14161 ttgcttggca aaccttcgac cttcggtatg cagatcaaaa attatcctaa tctgctaagt
    14221 tctgccttgt ggaaggtgga gaataaaagt cgtattcaat ccgggaaacc tttgatattg
    14281 aagtttgacc ggaaaattga acaaaacaaa ctgaaggttt ttgtctttcc cgataaatct
    14341 ttcaacatga agtcagacag cattatacag atcagttcca aatccgacaa aacagaaaaa
    14401 agcgggactt catcaggtaa tataatcgtt gtgaataaaa atgaacttac caaagcgctt
    14461 gagaaaaccg cttataacac taaatcagtt gaaagcagca aaggaaacat gtacgcaaat
    14521 gacagattca gatcaaagat tcaacccctg aaaccaaaat cacttgtaat tccgtctccg
    14581 cctcttacgc ccgttacttt cagaaaaatt gctgaagacg aaccaagaat cggatactta
    14641 ctccaaatca gacgtaacag ttcattcgac cctgaaaaac atgaaatagc gtttgacccc
    14701 ggcttaaaac tttactttaa agacatttcc acctggtcag attccgaaaa aacagtattc
    14761 ctgctggaac cacgcaacca gctgatatct ttattaatgc cggatacact cgaacttaat
    14821 ctgaaaatta attcaaaagt cagcgaaaac acttgcttcg cggataatga cagaattatt
    14881 atcagaaccg cgacagcaaa aggtaaagtt cctgttactg aattgataat cagattattt
    14941 tcaggaatat tgtttataat ttacctgata ctgttgagct gtaaagcccg atttaagaaa
    15001 ggagccatta tttcttatcg gtcggtggat gaatcagagc tgtgcatcaa tatcacctta
    15061 cggaaaaaag gaataacgaa ctggctcaac cgctggttga atccgtttat accggaaaat
    15121 tctgaaattg ttttcgcgaa gatggatgcg aaaattcttt ttactgcctg cagaaaaaaa
    15181 acagcagtca ggattcacaa atcttttctg gacaacagac acttatattt agatcaccat
    15241 gcgcggaaag tcaaaaatta cgcctatatc acagaagaca acaaactgat aatcaaaaca
    15301 aataattcct acaccggaaa accggccgtc atcacttaca atcggatgaa aaatgttcgt
    15361 gacgatgtac cagttttcag gtttttatta ttcctttccg gaattatcct gatggcggtt
    15421 ttggttttaa tctaagctgt gacagtcgca gctttggcag aaatctttaa aataaaaaag
    15481 cgctaaaact catatttcaa agttttagcg ctaaatcctg gcggtatgga cgggactcga
    15541 acccgcgacc ccctgcgtga caggcaggta ttctaaccag ctgaactacc acaccaggct
    15601 tttttgttat ttgagtaagc ttgtttacct cgttaggtag ttgttttctc aaatgctgtg
    15661 caaagatacg gcaaattttg atatctgcaa taggtacaac aaaaaaaatc gaaaaatatt
    15721 ttgatttttt ccttttaaag caactctgaa gctattttga agccatttgc catgttgtgt
    15781 gtgcattttt tttgttatta tcaaacagtt tttaaataat taattctacc tttggaattt
    15841 tgaaaaagta cagaacctgt taaaatagaa tgacatggaa ataactagaa tatttgatat
    15901 cctggaccga tatttggaaa agtttcccaa tcaggatgca gcattagcat gcaaacggga
    15961 tggacaatgg caaaaattca gcattcagaa atacactgaa cttaccaatc tgatgagttt
    16021 cggaatgctt gaaatgggca ttaaaaaagg agataaagtc gggattgtaa gtggcaacag
    16081 gccagaatgg aatatgcttg attttgctgt gatgcaggta ggcgctattt ccgttcctat
    16141 ctacccaacc atcagtcagg aagattatcg gcacatactg aaccatgcag aggtaaaaat
    16201 gatttttatc gaaggcaaag acctgcgaag ccggcttgaa ccaatcttgg aaacactctc
    16261 atctttgaag gaaattttca ccttcaacga agaaaataat caatacaaaa aactggatga
    16321 gcttatagaa cttggacgtc agcatccgca caacgacgaa cttaccaaaa tcaaagcaag
    16381 tatagaatcc aatgaactag caacaatcat ttatacatca ggaacaactg gagtgcaaaa
    16441 aggcgtaatg ctttcgcaca gcaatctggt taaccaactc ctgaacctga aagacacacc
    16501 catgaagtgg agcaacagag cattaagctt cctgccacta tgccacgctt atgagcgaat
    16561 acttgtatat ttatatcagt atctcggcat gtcggtctat tatgctgaaa gcctggctac
    16621 tattgctgaa aacatcaagg aaataaatcc aaccatgatg acctgtgtac cccgacttct
    16681 tgaaaaaatt tatgacaaac ttcactcggc tggtcgcgat ctgtcttttc cgaagaaaaa
    16741 catctattac tgggcatttg atctggccac caaatatcaa ctggaaggaa tgagtaaagc
    16801 gatgctgtat aagctgaagg ttgccgataa actgatatac tccaagtggc gcgaagcaat
    16861 cggaggaaat tttgacatcg tagtgtcggg aggttctgcc atccagcctc atatggcttc
    16921 gtttttttca gctatcggaa tgccggtgtt cgaaggttac ggcatgagcg aagggtctcc
    16981 cgtgattgca gtcagtcagc gtggtgtgaa tggacgtaag ttcggaaccg taggaccacc
    17041 gctgcccgga gtggaaatcc ggataatcga cagggacgaa atcgtgttcc ggggtcacaa
    17101 tgtgatgatg gggtattaca aagatcccga aatgaccgct caggtgattg ataaggatgg
    17161 ctggatgcat acaggcgata ccggcaaatt tacgcccgaa ggtcagttgg tcatcaccgg
    17221 tcggttgaag agtattttca aaacttcttt tggtaaatac gtcaatccgc aaacggtcga
    17281 atccaaattt atcgaatccg gttttatcga gaatctgatt gtactcggcg aaaacaaaaa
    17341 gtttgcagca gcacttattg taccggattt tacttatctg aaggtctggt gtaaagagca
    17401 caaaatacct tacaccacga atgaggaaat gataaaacat cccgatgtga taaaacgctt
    17461 tcaaaaagag ataaaacact ataatcattt ctttggtgat tacgaacaaa taaaacgcta
    17521 tcagtttgtt tccgaagaat ggtcgcaggc aagcggcttc ctgtccccca ctctcaaaat
    17581 taagagaaat gtaatcgaaa acttctacgc cgataaaatt gccaaattat ttgattaatg
    17641 taatcatttc aggaagtcac tttaaagaac ttacacagct tttcgcgcag accggagagc
    17701 tgtgtttttt tgataaattc acagtattac ggtagtataa attgaattaa tattgtttaa
    17761 cccccaaaat aatttacatt ttttacccgt taaacattgt tagttataaa aattaatttg
    17821 cgtatatccg atttttttta tatatctttg caagtgaata aaatataaac actcagcaag
    17881 gatggttaag cagaaaaaaa attcaaaaat tttatttata tctcaggaaa tatttccgta
    17941 tttacccgaa tcggaaatag caaatcaggg tcgttatttg ccacaggcag cgcaggaaag
    18001 aggtaaagaa acgcgcactt tcatgccaaa attcggactt gtgaacgaac gacgcaacca
    18061 gctgcatgaa gtcatccggc tttcgggtat gaatttaatt attgacgaca ccgatcatcc
    18121 gctgatcatt aaagttgctt ccatacagtc ggcccgaatg caggtttatt ttattgataa
    18181 tgatgattat tttcaacgga aaaatacgat aaaggatgat gatggagtgg aatatgacga
    18241 taattacgaa cgtaccattt tttttgttcg aggtgttatg gaaacagtga aaaagctgcg
    18301 ctggacaccc gatgttattc actgtcaggg ctggatgtcg gcattagcgc ctttgtacat
    18361 caaaaaggcg tataacgagg atcctttttt caaacattcg aaagttgttt attccatatt
    18421 caatgatgat tttattaaac catttcatgc tcagttcgct gataatcttt tgctggaagg
    18481 aattcacaaa aaagacctgg caagtatcag cggcgaggaa attgactacg tagccctgac
    18541 aaaactggct attgattact cggatggggt aatacagtcg agtaaatcga taaatcagca
    18601 tctgactgag tatgtggctg agaaaaaaat aaaattttta ccctatgtgg atatcgaaga
    18661 ttacgcggat gattatctga atttttacga tagcttataa tatctttcaa aaaaaatatt
    18721 tactactaat tccttactca atgaagttta aatttgtgtt ttcgctttta atactggcat
    18781 cagttttttt tgcttcgtgc gatgactcgg cattaaatct gggcgagaat atccaaccga
    18841 cctccgataa aatttcaata aaggctgata cttttcacct gactactgaa acctatatgg
    18901 tggactatat ctattccact cccgattctt ttctgctggg aacttatatt gacaatgtgt
    18961 tgggcacaac cagagccgat atcctgaccc agttttacta tccgaccgat tataaattcc
    19021 tcgataaatc ggtagcagta gcaacacccg attctgtaaa actttacatg acttttgatt
    19081 cttattttgg tttgagaacc tcaccgatgg aaataagtgt ttatgaatta caggattcgc
    19141 ttagaatgaa agaaaactac cgttctgata tcaatccggc aaattttgtg aatttcggga
    19201 agcgcctgaa cgctgaaaat gaaggcattt ttactgtcta tgacgttaag gatgacgcag
    19261 ataataccag tgtatccgtc aaactgaata atgatttttt aaatcgcttt ttcacctccg
    19321 atccggctgt gtttgccagt gaccgcaact tcctcaattt ctttaaagga ctttacatca
    19381 ctaccgattt tggaagcacc acactgctca atgtcagcgg agtgtacctg atgctttatt
    19441 atcacaatac gtattcaaaa gacggaagta ccagcaaagt aaccctgaca ttcccggcaa
    19501 gtaatctgga ggtaaaacgt gtaaatcgca ttacacatcc atcacgttcg gctaatttca
    19561 cacagaatga cggaataaat tatatctcat cacccgcaaa ctgctacacc cgcgtgaaaa
    19621 ttccgataga aaagatgcgc agcagactca ccttacccgc taataaacac cgtataatta
    19681 acagcgctat cctgcaggtg aatgtggttg acaaagacac tatcggaacc cgcttgccct
    19741 atgttaagaa tatgttgctg gtaaaagaag atgagttgga tgattttttc aaaaacaata
    19801 aactacctac atcaggcagt tcttatctgg ttagtctcga cagcacgagc cttacatcga
    19861 ctacttacga atatcattat actttcaacg gattaagtac ccttattgat gcagaactga
    19921 agaatccagc agaaagtcat aaggacggat atgtaaatat ggtactcgta ccagttgatg
    19981 tcaacaaaac caccacttcc tcctactatt catcaagcac aacgattaat tcagttcgac
    20041 agaacaccca attacaagct gtcggtgttt atagtggtaa aaacaagaaa atacccatga
    20101 aaatggaagt cgtttacagc ggcttctgat cggccccagg cacgatagaa aaaataatat
    20161 aaaaaaagca gcaagacgaa atggttttgc tgcttttatg ttttgtgatg aactgaatat
    20221 tatttctgtc tgataaatac attaattggt gtaccatgaa agtcccagtt cgctcgtatc
    20281 ttattttcga gaaagcggcg gtaaggctct ttgacgtact gtggcagatt ggcaaagaaa
    20341 acaaacgtag ggatgtaagt tccgggtaat tgggttacgt atttaatttt gatgtatttt
    20401 cccttaagcg caggtggagg ataattttct atcagaggta aaagcaactc gttaagttta
    20461 gctgtaggca cttttcgttg tttgttctca aaaacccgca cagcagtatc cagcactttc
    20521 agtatgcgtt gctttgtaag tacggaagta aaaatgattg gaaaatcaac aaaaggagca
    20581 atccgttcac gaatggattt ttctattttc ttgatatcat ttgcttcctt gttatcgata
    20641 aggtcccatt tattgacaca aaccaccagt cctttcttat ttttctggat aagtgagaaa
    20701 atgttgagat cctgactttc gagtccgcgg gtagcatcga gcataaggat acatacatct
    20761 gaattttcga tggcccgtat tgaccgaacg acagagtagt actccaggtc ttcgtttacc
    20821 ttggcttttt tacggatacc ggcagtatct accagataaa aatcatggcc aaacttattg
    20881 aatcgcgaat atatactgtc acgggtagtt ccggcgatat ctgttacgat agtccggtct
    20941 tccccgataa atgcattaat cattgacgat ttgcccacgt taggtcttcc caccacagca
    21001 aagcgtggca attccagttc aatttcttca ctgacatcgg gtttgaaatg cttcatcagc
    21061 tcatccagca gctcaccggt acccagaccg tttatggccg acagaatgaa aggatcaccc
    21121 agaccaagtt tataaaactc agcggcctga tattgcattt cgaaagtatc tgttttgttt
    21181 gagacaagga taaccggttt gtcggctctg cgtaaaatct gtgctacatg aatgtccaga
    21241 tcggtaactc cgttttgcac atctaccaga aacaggatca catccgattc ttcgatggca
    21301 agcagtacat gtcgtttaat ttcttcttca aaaacatctg tggagttgac aacccagcct
    21361 ccggtgtcga tcactgaaaa ttctcgcccg caccattcgc tttttccata ctgacggtcg
    21421 cgtgttgttc ctgcttcttc gttaacgatg gctctgcggc tattggtaag cctgttgaaa
    21481 agtgtggact tgcccacgtt ggggcgtcct acaattgcta caatatttcc cattgttttt
    21541 ttgtttagtc cggtcactgt tgtttccgaa cggtttaact tacattttat ttgtttctgc
    21601 ccgtttcgtc gcgaaacagt acagtgggta caaatattcc gaatgcaaag gtacaataat
    21661 tttccggata aatccgaaat gaatatatat tgctcgttat gtgcaaatta gcaatcattt
    21721 ttaataacag atagcacatc aataaaaaag ggatgtatat cacatccctc ttttaaattg
    21781 ttattaagtt tcttatttca gcattttatc gacggcgttt atattgtcaa tcggtaccag
    21841 ggtaaaacca taagaaatat ttgtttcttt cggaataatg cggtatggca acataggcca
    21901 ttcgccccag ctgttgttgc caccaacgcc catcatgcgg taatcgataa acaaatccac
    21961 tttgtctgat ggtttgtatt catttatatg cttgatttta ggcgcagtgc ccactgctgc
    22021 atcgttgttc cagtcttcag cattggctat tgtttcgagc aggtagttgg atacgttgaa
    22081 ctggaatttg tcatcggcaa aaaatgccat tcccttgcca gatccaagtg taactgccag
    22141 ccaggctgca tctgtatgat gcgcattctc ctgtgtcatt acatatttat ccaccatgtc
    22201 gataatggat gatttgtaac ggtcaacgaa cgtggaagtt ttcctgtctt catagtttgc
    22261 ccagggacct ctgccgtaat attcagcgtt tacaaattca cccggaagtt gcatacgcat
    22321 cccgatgcgt ggaataagcg gaaaatcgca tttggttgca tctacagcgt tttccacatg
    22381 gatagagccg tttttataaa tcgtgtaaat taccttccag cttgtattgg tttgagggaa
    22441 acgataatca cagctaacaa tcgtgttttc accggttttt acattcagat tttccgcttt
    22501 taattctgta tagcttgctt ttttccagtt atttaatttt tttggcagtc ctgctccgta
    22561 ttcattatct gtggatgctc tccaaaagaa aggtttcaga ccgaaatgat cgctaatata
    22621 ttcagttcct ttgtatttat acgaagtcat cacacctgat tctttatcaa aaatcaccga
    22681 aaaatctgtt ccgttgaaaa taagatttgc tgtgttttga gtaaccgtag ccggtgattt
    22741 agcgtttaac accattttcg ggtaatcatt gacgataaac tggtcacgtg caacgaccca
    22801 tcctgcagga atatacctgg tatctgtttt ctgtctggct tcaaaattaa tggtaacctg
    22861 attttttact ttaaaatatt ttgaaatatc aggaatgaca acggttttag tatcgtgtgg
    22921 ttttacattg agagctatct ggcctgtttt cagaaccttg ccatttgatt ttatggtgta
    22981 atgaaaactg tactggctta aatctatgaa aaagttttca ttataaacat cgacagtacc
    23041 tttctgtgca tcaaatttct taaaccagat attctgataa acttttcgca tttcttcggt
    23101 gtgtggtttg gtcgtgcggt ccggcagaat caatccgttg cagcagaagt cgcccgatga
    23161 gggtgtccct ttcggtccga aatcccctcc aaaagcccaa aacttatttc cttccgcgtc
    23221 tttttgggct atcccctgat ccacccagtc ccagatacat cctccctgca gcagcggatt
    23281 tgatcgaata atattccagt aatcaataaa gttgcccaga ctgttgccca tggcgtgagc
    23341 atattcgcac aatatgagcg gacggtcaga attctgattt tttgcataac tttcgatttc
    23401 ttctggtctg tcgtacatcg gacagaaaat atcggtgttc cattcaagtc cggcacgctc
    23461 gtactgaacc ggacgggagg tgtctctgcc ttttatcaga ttgtatgttg cgtacatatt
    23521 gtaaccgttg ccggattcat ttccgagcga ccacgttaca acacacgcgt gatttttatc
    23581 tcgttcaatc atttcggagg tacgggccag atgcgcttcg aggaaaagcg gattgttggc
    23641 aagtgttgcg cctttacgta ggtcataacc cattccatgc gcttcaatat tagcttcatc
    23701 aataacataa atgccgtact cgtcagccag tctgtagaat aattcctgtt gaggataatg
    23761 acaggtacgc acggtgttaa cattgtattt gcgaaacaat tcgaaatctt tccgcatcag
    23821 ttcttcgctg acgtaatgac ctgtaaattc gttatgttcg tggacattta cccccttcac
    23881 taatacaggc tgtccgttga tcataaactg tttgtttttg atttcagaag ttctgaatcc
    23941 aactttaacg gctgtagctt ccagggtatt cccgttttta tctttcagtt cgatactcag
    24001 cgcatagagg ttaggctctt cggctgacca ttttttcaca tccggaattt ctttcaaaaa
    24061 tttaacctga actttgtctt ttacatttat cgttttcgtt tcggaagcgg tcatcttacc
    24121 agtggcatct ttcagtgtgt aagttacagc aagctcgtca cttgttattg cactgttttc
    24181 aagagcaacc tccagactga attgtccgtt ggtatacgtg gcatccaatg gtgtatgtgc
    24241 aaaaaaatcg gctatgtgaa cctttggacg ggcataaaca tatacatcgc gttcgaaacc
    24301 ggaaagccgc cagaaatcct gacattccag atagttggca tctgagaaac gatgtatctg
    24361 aacagccagc acattttttc cggcttttac ttttgctgta atgtcaaagc gggcaggaag
    24421 tttaccggcc ttattcatac ctataaattc gccattgagg taataataag ctgcaccttt
    24481 agctgcatca gcactcagga ttatctcctt tcctatccac gattgtggaa tttcaaattc
    24541 acgacgatag gtaccagtcg gattaaattc ctttggaacg tatggaggat ttggcctgtt
    24601 ccagaaaggt tcgtgtccgg gcgaagtaaa ttcataagtt gtattaacgt agattgggta
    24661 tccaaaaccc tgaacttccc agtttccggg aacttttatg tctttccagc ttgatgcatc
    24721 aaaattctcg ttgtaaaagt cattcattgg ccgtttactg aaatcttcag catagttgaa
    24781 tttccaggta ccgtccagtg aaataaaatc acttccttta gaggagtaaa gagctttttg
    24841 agcctcagca acattactga atgaaaagaa gctgctatga gcaggtaatt tattaataca
    24901 tgtgagtttg ggatttgtaa tttcgtcata cctgttttcc tgcaattgag caaaaacacc
    24961 aaacgtgatg aagatcccca ttaatagtat gaatgagcgt tttttcatag ttttgatttt
    25021 ttatattact gattattatg tgacttacaa agatactgaa taattccgta ttcaaaaaag
    25081 ccaaatatta cattttcagg caggataaaa gcgtctgata atcagtatcc aaagatgtat
    25141 cgtggaatat atttcagaaa attattcgaa tttagcagta aataattttg taaaatgcat
    25201 gaaataggct atctttgcac cgctaaaaaa taatggcgag atagctcagt tggttagagc
    25261 gtcccgatat aaaatcggga aggtcgggat caactaagtt ataattgaaa taatggcgag
    25321 atagctcagt tggttagagc gcatgattca taatcatga
//
LOCUS       DAOPYB010000025        25037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1979_length_25037_cov_10.596109, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000025 DAOPYB010000000
VERSION     DAOPYB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 25037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25037
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1979_length_25037_cov_10.596109"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1942)
                     /locus_tag="PKH58_03495"
     CDS             complement(<1..1942)
                     /locus_tag="PKH58_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNX88121.1"
                     /translation="MIKTKFFILTLALVLISGIGSDIYGAKKKKVAVKQVPVLTLEQR
                     VDSVMATLTLEEKVGQMAQYTLDVIGREAKPAVSPAEGPGTSVDPFEFDPAKLDLILG
                     KMKVGSILNTTNNKAQTTKMWAYIVKIIQQRAIKETGVPVLYGIDAIHGTNYTAGSTL
                     FPQGVNMGAGFNTALMEEGSKISAYETRASNIPYTFAPTMDLTRDQRWSRHWESYSED
                     SYLTAKMALASLRGFQGNDYNKIDKNHVAVSLKHFMGYGVPVYGKDRTPSVISENDLR
                     EKFFEPYRQAMKAGALSVMINSGVINGVSTHADYRLLTEWVKEDLQYDGVLITDWNDV
                     ENLYKRDRIAVSFKEAVCKSINAGIDMVMVPYNLDFCSALIELVQSGKVSQARVDDAV
                     RRVLRMKFRLGLFERPYWDLTEYPDFGSKAHEASAKRAADESITLLKNENNLLPLKKG
                     VRLLVAGPNANSMRTLNGGWSYSWQGEKVEMFAQQYNTIFEALKNEFGANNVKYEAGV
                     TYKMEGQYFEENTPEIEKAVAAATDVDYIVVCLGENTYCETPGNLDELALSENQINLA
                     KALQKTGKPVILVLNEGRPRLIRTIEPASKAIVQIYLPGNYGADALADVLSGDVNPSG
                     RLPYTYPKFEQGLITYDHKPSQN"
     gene            complement(1996..3108)
                     /locus_tag="PKH58_03500"
     CDS             complement(1996..3108)
                     /locus_tag="PKH58_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNX88122.1"
                     /translation="MKTMYNLLLIVLVLFSGCGNNDGPDPEPPKTEITWQFAPENITF
                     TDDGNTQNLTVTSNGSWTATSSQSWCTITPASGYNGQTSMKITLSKNGMNTERTAVLT
                     FKSGTYTKKLSVKQAAATIPNYVPAGYTLKWQDEFNDSRQSNGKTPLPSLARWWYETG
                     ANGWGNNEIQNYISAYRGTDTCAVISDGILKIIAKKVGTEVLSVRMNSTDSWTYGYFE
                     ARLKMPTGKGTWPAFWMMPKNFTNWPLDGEIDIMEYVGYDPNVVHASVHTMAYYHTIG
                     TQKTATKTILNAETDFHIYAMEWTADKIDGYVDGVKYFTFNNDKTNNKNTWPFNAPFY
                     LKLNLAWGGNWGGALGIDATKLPAVYEIDYVRVYQK"
     gene            complement(3250..4749)
                     /locus_tag="PKH58_03505"
     CDS             complement(3250..4749)
                     /locus_tag="PKH58_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX88123.1"
                     /translation="MKNIIKNGIGVLLSFMLVTACSPQEDDKYSLGTLDTVTADQITF
                     SQTPSSTSANVITFTNTTAIKGVYSMGWDLGNGGTGNSNTVVGQYPFAGDYTVTLTLN
                     TPDGKSATKSQVIHIAQNDYALINTPAYVNLTGGATDLDGKTWVFDQYNNFAKEVAAA
                     TGFPISGHIGLGPQGSYGQSWWGAAPNDKGSWKMYDFKFTFIQNGVKLNIVNKGEGYG
                     RKASAASIGGYSVTGINGDDAIFDYNGGNYNFGLDESGKYPKLTLTGNAFMGYYCGSQ
                     VYEIIYQTDKVMALRVNNTVEGQDWVFVYCLPELNVAAPPIVKEPKAVPLSENFETGT
                     SKINFAKEDMGGKTGIFDNPSPVPINESDKVYRYQKSTAFYSNVSFTAEKYKFDLTKQ
                     NKIKLKVYIPSNDYTTDWATAGSWISNAKLRPQLAVKLQDSSMGGNAWQSQTEIVKGD
                     LELNKWIELTFDFSNVATRTDYDKIVIQFGAEGHAGTGIFFFDDFSFNE"
     gene            complement(4829..6367)
                     /locus_tag="PKH58_03510"
     CDS             complement(4829..6367)
                     /locus_tag="PKH58_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX88124.1"
                     /translation="MKKYIFIMFVALIGFTSCGEDFLTALPTEKQAAGGSATEGAILS
                     NLGSAYQILLFDSYANNNYNSIPLMSDLRSDDIYKGGGDAGDQHQLYLLSLFTTTPNE
                     NIDGLWSIFYTGLARCNNVIIACDNAVNVPEAKLNQYQAEAHFLRAYYVHWLWKFWGN
                     VPYFEAPLSSAPYMAKQLTANEVYAAIMKDLDFACGKTDAGADRLPMSYSKGSTSTGR
                     VTKAAALMLKARVVMYQKDVARYAEVTKDMAEIIKSNEYGLFNNYAAMWVDENEFCQE
                     SIFESNQLPEGKTWSSGWQGYGTNLPAFISPNGLNTGSKTGDFKGGWGFGPVRQATWD
                     MYEEGDTRREGSINKWEGSQYTARFQDTHLFQAKYAARVGYNPQGDVDLNYCNNFRIF
                     RFAETLLNYAELVVVHGQPAVDGITAQSCLDRVRNRAFGTDKPIPATVANIKLERRRE
                     FVGEGIRFWDIVRWGDTSLLTESTPLSTRTWNDNKKYLPIPQSEIDKTKGTEFELKQN
                     PGYN"
     gene            complement(6389..9865)
                     /locus_tag="PKH58_03515"
     CDS             complement(6389..9865)
                     /locus_tag="PKH58_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX88125.1"
                     /translation="MRKQLFKRRTFILFTLMFIGLSNMGFAQKVSLNSSQQTLKQVLE
                     SISRQTGYTLAYSKEVVNLNEIVNINVSNASLSDVMTKLLSSRNLSYEIRDKKIYIAG
                     SQAPKLTSQQNQPTVLFQGKVIDEKGDPIIGANISIPNSSIGTVSNATGSFSLSVPAG
                     SVVHVTYIGYVEQQFTVNSPSTMNITLKEDTKSLNELVVIGYGVQKKSVVTAAISRVT
                     AEQLNNTKPSRVEDVLKGKVSGVQITQSSGQPGSDSKVRIRGIGTVNNSEPLYIVDGM
                     PVDGGINYLNPVDIQSVEILKDASSAAIYGARAANGVVLVTTKNGVAGKTKINYNVSY
                     GWQNPWKKKSVLNAQEYMVIMNEGLINDGGLPRYNNEQVMNAGTGTDWQDETFNYNAP
                     IKNHQLSVSGGTERSQYFLSLGYFDQQGIVGGDFGKSNYNRWSVRSNSTHTIFEEKSR
                     DFLNKLRIGVNLGYSRDKSSSIDANSEYGSILGSALTFSPLVPVYADEETAAAILAKY
                     PNAVKDKDGRVFSIPPSGFQEIANPVAMLNQPSSGLNNSDKIVSTFWGELSVLPELKF
                     KSSYGVDLAFWGYDSYTFPYFLATQGKTVDVSTVQSEMNRGFKWQVENTLSYNKTFGG
                     KHNVAAVLGQSAQKYTYRSLGGNDQQLLETDPLKANINSAIADRKLERVWGGTGGYNQ
                     ATLASYFARLDYNYAERYILQATVRRDGSSNFGPNHKWATFPAFSLGWNLTNEEFLQD
                     RPKWIDALKLRFSWGKNGNERIGAFRYTSLMDGGQNYYFGGGYKVNNGDPTKVGETGG
                     VMQYGSSPASIPNPDVKWEESQQTDIGFETYLFKSAFTFSFDYFKKITNGMLMEQPIP
                     SYVGQGSPIANVGDMQNWGLEFETGWKQTAGDFKYSVSANASYLKNKLIKLGNATGEQ
                     IYENAGASGVGSYVKGKNGEVFPYFYGYKTDGLFQNQAEIDAYVNTDGQKYQSAAKPG
                     DVRFVDANGDGIISDEDKVKIGKGMPDWTFGLTLNAEWKNFDLNIFFQGTLGNDIFDF
                     SQRGDIPAMNRPEWILQRWHGEGTSNSIPRMTIANANSNWRSSDLYIKDGSYARMKST
                     QLGYTLPVSMTRKVSIENLRIFVMAENLLTLTKYDGFDPEVASGGYTTIGIDRGIYPQ
                     SRTISVGANISL"
     gene            complement(10102..11091)
                     /locus_tag="PKH58_03520"
     CDS             complement(10102..11091)
                     /locus_tag="PKH58_03520"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNX88126.1"
                     /translation="MHPFEKETIDKVIEGIASKKEAREVAMWFSETVEGQAYLSHLLD
                     CEAGVLDENPNKYITLSSVQSANILSEINRQIRHKRMRKIYWQAAAVLIPVIMILGIT
                     FQLNNQVNLFGKTVYAEVYVPKGKQEHIVFQDGSEAYLNADTRLRFPRKFGLSKREIF
                     LQGEGYFNIASNKRRPFVVNINNTNVTVLGTSFNVHAYQEEREMKILLDKGNIIFNTP
                     CDSYQILPGQQAVYDVLTGKCTIDYIDRPYEVSVWKDNIILFHDTPLADVIKVLNRKF
                     NVDFNVQDPKALTYTYTLTTNQTSLENIIKELQKISPVKFSTTKFNSVNIYLK"
     gene            complement(11158..11736)
                     /locus_tag="PKH58_03525"
     CDS             complement(11158..11736)
                     /locus_tag="PKH58_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNX88127.1"
                     /translation="MRTQFSKYADNEDAALFQLIKKKDKEAFTIVYYKYHQYLYNLAY
                     RYLKDTEMSGDAVQQVFVKLWVSSPFIEIEINLKNYLYTMTKNFILNQIRDNKEMISL
                     NYQNNQIEISDDSDILKMLEDNQLSNFLYEGINNLPSQKMEICKMKIDENKSNQEIAD
                     AMGISVNTVKSHYQESVKMLRDYFRKIKLMFL"
     gene            complement(12115..12912)
                     /locus_tag="PKH58_03530"
     CDS             complement(12115..12912)
                     /locus_tag="PKH58_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88128.1"
                     /translation="MSAPIFNKENYLIKAEHYPATNYREKIILQQIELIDKPDIRLEG
                     LYYLILWDLFLIGDREDASYKERLRLARLLKFLYSEEGGVILTGKDNPDNTSEISKKQ
                     IPEFQEVVKKQLSETFLMVRLNEKSEFHFPRKTWSCENASQWPPDKRKIEVLETTTFA
                     MNYMKKNKSIKIVIDRENIDLIISMEAERELDEKKTYVGRFLSFINKYAIKYGYFSQI
                     KNSISIQDALFLYETLELLEIIQPDILHDEQEKYLFIKRELKNGNDF"
     gene            complement(13144..15330)
                     /gene="recQ"
                     /locus_tag="PKH58_03535"
     CDS             complement(13144..15330)
                     /gene="recQ"
                     /locus_tag="PKH58_03535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNX88129.1"
                     /translation="MTKHSELTSYLKEYFGFDTFKGNQEAIIQNVLDGKDTFVLMPTG
                     GGKSLCYQLPSLLMEGTAIVISPLIALMKNQVDAMRNFSEEDGVAHFLNSSLTRAAID
                     VVKEDVISGKTKLLYVAPESLTKEENIEFLRQVKISFYAIDEAHCISEWGHDFRPEYR
                     RIRPIINEIGVAPLIALTATATPKVQHDIQKNLNMLNATVFKSSFNRPNLYYEVRSKT
                     KDVDKDIIKYIRSQAGKSGIIYCLSRKKVEELAETLQVNGISALAYHAGMDSLQRSEN
                     QDKFLMEKADVIVATIAFGMGIDKPDVRYVIHYDIPKSLEGYYQETGRGGRDGGEGQC
                     IAFYAYKDLDKLRKFMQGKPVAEQEIGNQLLLETQAYAESSVCRRKLLLHYFGEEYTL
                     ENCGSCDNCMKPRKFVEGKELLVLVLETVQQLKEKFKAEHIVDVLKGNESPDVKSYQH
                     VDLENFGSASDEDEKLLHAVIRQAMIAGYISKDIETYGVLKMTASGKEYLKNPKSFPI
                     VKDTEFEDDEDDMSSRNAGGTCAADDVLFSILKDLRKKLSKKLEVPPFVIFQDPSLEA
                     MATSYPISMDELQNIPGVGAGKAKRYGEEFLKVIREHVKENEIIRPEDLRVRTVANKS
                     KLKVSIIQSIDRKIALDDIALSKGLDISELLDEIEAIVYSGTKINIDYFLDEVMDPDR
                     VDEIFEYFNSAETDKISTALDELGENDFSETEVRLVRIKFLSEIGN"
     gene            complement(15566..16798)
                     /gene="clpX"
                     /locus_tag="PKH58_03540"
     CDS             complement(15566..16798)
                     /gene="clpX"
                     /locus_tag="PKH58_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNX88130.1"
                     /translation="MAKNSKHCSFCGRPESQVGFLITGLDGAQICNECTEQAAAIVRD
                     AKMGNDMASTLDKEKLPKPAEIKNYLDQYVIGQDDAKKYLSVAVYNHYKRVLQEKSND
                     DVEIEKSNIIMVGYTGTGKTLLAKTIARKLHVPFTIVDATVLTEAGYVGEDIESILTR
                     LLQVADYNVKEAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLEGSVVNV
                     PPQGGRKHPEQKFIPVNTQNILFICGGAFDGIERKIANRLNTRVVGYGASIENEMVDR
                     ANLLQYIAPQDLKSFGLIPEIIGRLPILTYLEPLDRSALRNILTEPKNSIIKQYVKLF
                     KMDGVDLIFEPEVLDYIVDKAIEFKLGARGLRSITETIMIDQMFEMPTKKEKKWVVTI
                     DYARTKMDKANAHRLKTA"
     gene            complement(16802..17479)
                     /gene="clpP"
                     /locus_tag="PKH58_03545"
     CDS             complement(16802..17479)
                     /gene="clpP"
                     /locus_tag="PKH58_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNX88131.1"
                     /translation="MNNNDFRKYATKHLGMNGLSLDEYFKVMNNGYISPSILEERQLN
                     VTQMDVFSRLMMDRIIFLGTQVDDYTANVIQAQLLYLDSADPGKDISIYLNTPGGSVY
                     AGLGIYDTMQFISSDVATICTGMAASMGAVLLVAGKNGKRSALKHSRIMIHQPSGGAQ
                     GVAADMEINLREMLKLKKELYQIIADHSGKTYEQIEKDSDRDFWMTSQEALEYGMIDR
                     VLIKEKK"
     gene            complement(17671..19032)
                     /gene="tig"
                     /locus_tag="PKH58_03550"
     CDS             complement(17671..19032)
                     /gene="tig"
                     /locus_tag="PKH58_03550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNX88132.1"
                     /translation="MKFVRKDLDQTKAVITVSVTKDDYNENVEKYIRNYRKKANVPGF
                     RPGNVPVGLIKKLYGKAIVAEEVNKLVSDGLFGYVKENNIKFLGEPLPVENHHDVDFE
                     NSEDFDFDFEIGLAPQFDVDLSKSDKITYYNIKVSEQMIDNQVKSYTSRFGKYTQVDV
                     VEENDMVKGELLEMANGKVNEDGLKVKDAVLTPAYMKNADQKALFVGKKIGDKVVFNP
                     QVAYENEVEISSMLKISKDEAKKITSDFMMQIEGITRYEEGEINQELFDKVFGEGAVT
                     SENEFRQKIAESIKENLTADSDYKFGIDAQAALSAKYNDLKFPDKFLKRWLLYSNENM
                     TSESLEEDYPRMIKDLTWHLIKDKIADANGIKVETADVQEYAKKVAKSQFAQYGMVGL
                     EDSIIENYAKDLMKKEETVKNFVDRAMEEKVLDYIKGTVKLNEKEISVEDFNKMFQET
                     GEN"
     gene            complement(19383..21152)
                     /gene="fucI"
                     /locus_tag="PKH58_03555"
     CDS             complement(19383..21152)
                     /gene="fucI"
                     /locus_tag="PKH58_03555"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006005 - L-fucose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HNX88133.1"
                     /translation="MKKYPKIGIRPTIDGRQGGVRESLEEKTMNLASAVAALISSNLK
                     NGDGSPVECVIADTTIGRVGESAACAEKFEREGVGSTITVTSCWCYGAETMDMNPYYP
                     KAVWGFNGTERPGAVYLAAVLAAHVQKGLPAFGIYGRDVQDAGDNSIPEDVAVKILRF
                     ARSAQAVATMRGKSYLSIGSVSMGIAGSIVNPDFFQEYLGMRNEVVDMTEIHRRISLG
                     IYDHKEYEKAMAWTEKYCKVNEGVDYNLAENQKTRTEKEADWEFVVKMTLVMRDLMQG
                     NPELQKNGFKEEAIGHNAILAGFQGQRQWTDFLPNGDFSEALLNSSFDWNGIREAFVL
                     ATENDACNGVAMLFGHLLTNTSQLFSDVRTYWSPEAVKRVTGRELTGMAQHGIIHLIN
                     SGSTTLDGTGQQSRDGEPALKPFWEITENEVEACLGATTWYPADRNYFRGGGFSSNFL
                     SKGGMPVTMSRLNLVKGLGPVLQIAEGWTVELETEIHDALNQRTDKTWPTTWFAPRLT
                     GSAPFKDVYSVMSCWGANHGTISYGHIGKDLITLAAMLRIPVCMHNVEEDEVFRPSAW
                     NAFGMDKEGADYRACKNFGPIYK"
     gene            21608..22789
                     /locus_tag="PKH58_03560"
     CDS             21608..22789
                     /locus_tag="PKH58_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX88134.1"
                     /translation="MKKLILFTSLLLSLTTFSVFSQGIQFEENHDLNAALAKAKAENK
                     LIFIDAYAVWCGPCKVMARDIFPLKEVGDYFNDKFVNLKLDMEATENVEIAKKYEVKA
                     YPTYLFLNADGEVVHKGLGSMPAEKFIEVAKAAADTENNFSALSKKIKNGDRSLETIN
                     KYLAQNPYDAGNGDLLDEYFKTLSENQINTKENWELFSNNIDKISSPWFQYFIANREK
                     FSALVGKDKTDAKIAKIMITAYNQDPSSEEKIKSIDPLLFEDVKAKVNLSKAYGQFRK
                     ANNMESWKSFINQAVTYYKDCKEANQLNEIAWMVFKNNSKFSDKELLKQGLKWASKAV
                     ILEPGNDAFLDTYANLLYASGKKKEAIKTETNALKIAEKAGNTEAVENYKKVIENFKN
                     K"
     gene            22844..24478
                     /locus_tag="PKH58_03565"
     CDS             22844..24478
                     /locus_tag="PKH58_03565"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNX88135.1"
                     /translation="MMAKPFIYQEAFPVGKEKTEYYLLTKDYVSVSEFEGQEILKVQP
                     EGLTALARTCMRDCSFLLRPEHQKQVAKILADPEASENDKFVAVTMLRNAEIAAKGVL
                     PFCQDTGTATVYAKKGNNVWTNGDDEEALSKGIYETYTNENLRYSQNAALNMYDEVNT
                     GTNLPAQIDLVASHGNEYKFLFIAKGGGSANKSYLFQETKALLNPVSLEKFLVEKMKT
                     LGTAACPPYHIAFVIGGTSADACMKTVKLASTKYYDELPTTGNELGQAFRDVEMEEKV
                     LLAAQKAGYGAQFGGKYFAHDIRIIRLSRHGASCFVGMAVSCSADRNIKGKINKEGIW
                     VEKLEDNPGQYIPEEYRQQGEGNAVKIDLNQPMPQILKTLSQFAVSTRLSLTGTIIVG
                     RDIAHAKLKERIDAGFGLPQYMKDHPIYYAGPAKTPAGMPSGSFGPTTAGRMDSYVDL
                     FQSEGGSMVMIAKGNRSQQVTDACKKYGGFYLGSIGGPAAILAQNSIKKVECLEYPEL
                     GMEAIWKIEVVDFPAFILVDDKGNDFFQKIKQHSMC"
     gene            24619..24999
                     /locus_tag="PKH58_03570"
     CDS             24619..24999
                     /locus_tag="PKH58_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88136.1"
                     /translation="MKNRITMGNDEFILYIRKNYNCSLTNDRLGRMIGKWILENSKGI
                     SKRNDIEHDIPCLWQQNIRAKKTYGTRLPRTASQITFNRNILPKLYDYLDFLGKKYVS
                     NQPWNEWKKREDETEDAGMLELNF"
BASE COUNT         7094 a         5369 c         4818 g         7756 t
ORIGIN      
        1 tattctgact aggtttatgg tcgtaagtaa tcaaaccttg ttcgaatttc ggatacgtgt
       61 atggcaaccg tccgcttgga tttacatcgc ccgacagcac atctgccaat gcatcggcac
      121 cgtagtttcc gggcaaataa atctgtacta ttgccttgga tgcaggctcg atagtacgaa
      181 taaggcgtgg acgaccttcg ttcaacacca gaattactgg tttacctgtt ttttgcaatg
      241 cttttgccag attgatttga ttttcggaga gtgcgagttc atcgaggttt cccggagttt
      301 cgcaataggt attctcgccc agacaaacta cgatataatc tacatctgtt gcagctgcaa
      361 ccgctttttc aatttcagga gtattttcct caaaatattg tccttccatt ttataggtta
      421 cacctgcttc gtatttcaca ttattcgcgc caaattcatt tttcaatgct tcaaaaatgg
      481 tattgtactg ctgagcaaac atttccacct tttcgccctg ccatgaatag ctccagccac
      541 catttaacgt tcgcatggag ttagcgttcg gaccagcaac caacaggcgt acaccttttt
      601 taagcgggag taggttgttt tcgtttttga gcagagtgat ggattcgtcg gctgcacgtt
      661 tagcagatgc ttcatgagct ttagagccaa aatcagggta ttcggtaaga tcccagtaag
      721 gacgttcgaa cagtccaagg cgaaatttca ttcgtaacac tcttcttaca gcatcatcca
      781 cccgtgcctg cgaaactttg ccagattgca cgagttcaat cagcgcactg caaaaatcga
      841 ggttgtaagg caccatcacc atgtcaatac cggcattgat tgatttacat acagcttctt
      901 tgaaactgac agcaatgcgg tcacgtttat ataaattttc gacgtcattc caatctgtaa
      961 taagtactcc gtcgtactgc aagtcctctt ttacccattc ggttagaagc cggtagtcgg
     1021 catgagtgga aactccgttg attacgcccg aattgatcat cacggacagc gctccagctt
     1081 tcattgcctg acgatagggt tcaaaaaact tctcacgaag atcgttttct gaaattactg
     1141 aaggtgtgcg gtcttttccg taaacaggta ccccataacc cataaagtgt ttcaaactga
     1201 cagctacatg attcttatct atcttattgt aatcatttcc ctggaaacca cgaagggaag
     1261 ccagtgccat cttagctgtc agatagctgt cttcactgta actttcccag tggcgcgacc
     1321 aacgctgatc gcgggttaag tccatggttg gagcaaaagt gtagggaata ttgcttgcac
     1381 gggtttcgta ggccgaaatt ttggaacctt cttccatcag cgccgtatta aatccggctc
     1441 ccatatttac tccctgcggg aaaagcgtag aaccggccgt gtagttggta ccgtgaatgg
     1501 catcaatgcc gtaaagtacg ggaactccgg tttctttgat ggcgcgttgc tgtatgatct
     1561 taacgatata cgcccacatt ttggtagttt gggctttatt gttggtggta ttgaggattg
     1621 agccgacttt cattttaccc agaatcaagt ccagttttgc gggatcaaac tcgaacggat
     1681 cgacacttgt ccccggacct tctgcaggac ttactgccgg ttttgcttca cgaccaatga
     1741 catcgagcgt atattgagcc atttgtccca ctttttcttc gagtgtcagg gttgccatca
     1801 cgctgtcaac gcgttgttcg agcgttaata cgggtacttg ttttacagca actttcttct
     1861 ttttagctcc atatatatcg cttccaattc ctgaaattaa aacaagagca agtgttaata
     1921 tgaaaaattt agttttaatc atttgtattt gatctaacag ttattcaata tgttgtttta
     1981 tcttgataat catcattatt tctgatacac cctgacataa tctatctcgt aaactgcggg
     2041 tagtttcgtt gcgtcaattc ccaatgctcc accccagttt cctccccagg ccagattgag
     2101 tttcagatag aaaggagcat tgaaaggcca ggtgtttttg ttgtttgttt tgtcgttgtt
     2161 gaaggtgaaa tacttcacgc cgtccacgta accgtcaatt ttatcagccg tccattccat
     2221 ggcgtaaata tgaaaatcgg tttcggcatt cagtatggtt ttggttgccg ttttttgcgt
     2281 gccgatggtg tgataatatg ccatggtatg aacagaagca tgaaccacgt tgggatcata
     2341 accgacgtat tccatgatat caatttcacc gtcgagcggc cagtttgtaa agttcttggg
     2401 catcatccag aatgccggcc aggtaccttt gccggtgggc attttcagtc gggcttcaaa
     2461 ataaccatac gtccagctat ctgtggagtt catacggact gacaacactt ccgtgcccac
     2521 ttttttggca ataattttca gaattccatc cgaaatcacg gcacaggtgt cagttccgcg
     2581 ataagccgaa atgtagtttt gaatttcgtt attgccccag ccattggcgc cggtttcgta
     2641 ccaccagcgg gctaaactgg gtaatggcgt tttgccgttg ctctggcgcg aatcgttgaa
     2701 ttcgtcctgc catttgagtg tatatccagc cggaacgtaa ttaggaattg ttgcagcagc
     2761 ctgctttaca gacaattttt tggtgtaagt acccgatttg aatgtcagca cggcagttcg
     2821 ttcggtgttc atgccgtttt ttgataaggt aatcttcatg cttgtttgtc cgttgtatcc
     2881 cgaagcaggc gtaatagtac accacgactg gctggatgtg gcagtccatg aaccatttga
     2941 agtaaccgtc aaattttgcg tatttccatc atcggtaaaa gtaatatttt cgggtgcaaa
     3001 ttgccaggtt atttctgttt tgggaggctc aggatctggt ccgtcgttat tcccgcatcc
     3061 ggagaataac accagaacaa tcaataacag gttatacatc gttttcattc ttattttgtg
     3121 tacgttgtaa ttatttaaac gcttattaca gagaaatagc ataatttggg tacaagtttt
     3181 tggtgaaaag agaaaaaagg aagggctaca accttgtagc cgcttccttt tttttaaaaa
     3241 aattagtaat tattcgttga aagagaaatc atcgaagaag aaaatacctg ttccagcatg
     3301 tccttccgct ccgaactgaa tgacaatctt gtcgtaatcg gtacgggttg ccacattact
     3361 gaaatcgaag gtaagttcaa tccatttgtt aagttcaaga tcacctttca caatttcggt
     3421 ctgcgattgc catgcattgc ctcccatact gctgtcctgt aatttcacag ccagctgtgg
     3481 acgcaatttg gcatttgaaa tccatgaacc tgccgttgcc cagtcggttg tataatcgtt
     3541 ggaaggaata tacactttca atttgatctt gttctgcttt gtcaaatcga atttatactt
     3601 ttcagcagta aatgaaacgt tgctgtagaa agcagtggat ttctgataac gatatacttt
     3661 gtccgattca ttgatgggta caggtgatgg attatcaaat attccggttt taccacccat
     3721 atcttctttt gcaaagttaa tttttgaagt tcctgtttca aagttctcgg atagaggcac
     3781 tgctttcggt tctttcacaa ttggaggagc agccacattc agttctggca agcaataaac
     3841 gaatacccag tcctgacctt caaccgtatt attcacacgt aaagccatta ctttatcggt
     3901 ctgataaata atttcataga cctgcgaacc acaataatag cccataaaag cattgccagt
     3961 aagagtcagt ttgggatatt ttccgctttc atccagtcca aaattataat tgccgccgtt
     4021 gtaatcgaag atagcatcat cgccgttaat tccggttact gaataaccac caatcgaggc
     4081 tgccgatgct tttctgccat agccttctcc tttatttacg atgttgagtt ttactccatt
     4141 ctgaataaat gtaaatttga aatcatacat tttccatgag cccttatcgt tgggagctgc
     4201 tccccaccac gactgtccgt acgagccctg tggcccaagt ccgatatgtc cgctgatagg
     4261 aaaaccggta gcagcagcaa cttccttagc gaaattgttg tactggtcga atacccatgt
     4321 tttaccatcc aaatcggtag caccaccggt cagattaaca tatgcaggcg tattgattaa
     4381 agcataatcg ttctgagcaa tatgtattac ctggcttttt gtggctgatt taccgtctgg
     4441 cgtattcaga gtgagtgtaa ctgtatagtc accggcaaaa ggatactgtc ctacaacagt
     4501 atttgaattg cccgttccac cattacccaa atcccagccc atggagtaaa ctcctttgat
     4561 agctgtagtg ttggtaaaag taattacatt cgcactggta gatgaagggg tttgtgaaaa
     4621 agtaatctga tcggctgtaa cagtatccaa tgttcccaaa gaatacttat catcttcctg
     4681 aggactgcag gctgtcacca acatgaagga cagcagcacc ccgattccgt tttttattat
     4741 gtttttcata cttgtttttt ttagttatcc atcgaaaaca ggtttgctac atgaaacctg
     4801 ttttcggcag gattgttgtt acaataaatt aattataccc cggattttgt tttaattcaa
     4861 actcagttcc cttggtttta tcgatttcag attgtggaat tggtaaatac tttttattgt
     4921 cattccacgt acgtgtactt aacggagtac tttcggttaa cagcgaggtg tcaccccaac
     4981 gtacaatgtc ccagaaacgg atgccttctc ccacaaattc gcgacggcgt tccagcttga
     5041 tgttggctac tgttgcaggt atcggcttat cagttccgaa agcacgatta cgaacgcgat
     5101 ccagacatga ctgagcagtg atgccatcca cagcaggctg tccatgaact accaccagtt
     5161 cggcataatt gagcaaggtt tcggcaaaac ggaagattct gaaattgttg caatagttca
     5221 gatccacatc gccctgcgga ttgtaaccaa cacgggcagc atattttgcc tggaaaagat
     5281 gtgtatcctg aaaacgtgcg gtgtattgtg aaccttccca tttgttgatc gagccttcgc
     5341 gacgggtatc accttcttcg tacatatccc aggtagcctg acgcaccgga ccaaatcccc
     5401 atccgccttt gaagtcacca gttttgctgc cggtgttcaa accgttgggc gaaatgaatg
     5461 ccggaagatt ggttccgtaa ccctgccagc cacttgacca tgtttttcct tcaggcaact
     5521 ggttgctttc gaaaatagat tcctgacaaa actcattttc atcgacccac atggctgcat
     5581 agttattaaa caacccatat tcgttactct tgataatttc tgccatatct ttggttactt
     5641 cggcataacg ggcaacgtct ttctgataca tcaccacgcg ggctttgagc atcagcgctg
     5701 ctgcttttgt aacacgaccg gtactggtag agcctttaga ataactcatt ggcaagcggt
     5761 cagcaccggc atcagtctta ccacaggcaa aatcaagatc tttcattatg gctgcataaa
     5821 cttcattagc agttaactgt ttagccatgt aaggagcaga tgaaagcgga gcttcgaaat
     5881 acggaacatt accccagaat ttccaaagcc agtgtacata ataagcacgc aggaaatgag
     5941 cttcggcctg atattggttc agctttgctt ccggaacatt cactgcatta tcgcaggcaa
     6001 taatcacatt gttgcaacga gccagacccg tatagaatat gctccataat ccgtcaatat
     6061 tttcgttggg agtagttgtg aacaacgaca acaggtataa ctgatgctgg tcaccggcat
     6121 caccaccgcc tttgtagata tcgtccgacc tgaggtcaga catcagcgga atgctgttgt
     6181 aattattatt ggcgtaactg tcgaacagaa gtatctgata agccgaaccc agattggata
     6241 gaatagctcc ttcggtggca gaacctcctg ccgcttgttt ttcggtggga agagcggtta
     6301 aaaagtcttc tccgcaggat gtaaacccga taagggctac aaacattata aatatatact
     6361 ttttcatatg tatcatatta ttttagaatt aaagagaaat gttagcaccg acagaaatcg
     6421 ttcttgattg cggatagata ccacgatcaa taccaatagt tgtatatcct ccggatgcta
     6481 cttcaggatc aaaaccatca tatttggtta aagtcagcaa attttcggcc ataacgaaaa
     6541 ttcttaaatt ttcgatggac accttccgtg tcattgatac gggcagtgta taacccagtt
     6601 gtgtagattt catacgtgca taagaaccat cctttatata aagatcggat gaacgccagt
     6661 tgctgtttgc atttgcaatt gtcatacgtg gtatcgaatt ggaagtacct tcaccgtgcc
     6721 agcgctgaag tatccattcc ggacgattca ttgccggtat atcgccacgc tgcgagaagt
     6781 caaagatatc attgccaagc gttccctgga agaatatatt caaatcaaag tttttccatt
     6841 cggcattaag tgttaaacca aatgtccagt ctggcattcc ttttccgatt tttaccttgt
     6901 cttcgtcact gataattcca tcgccgttag catccacaaa gcgcacatca ccgggcttag
     6961 ctgccgactg atatttttgt ccgtcagtat ttacatacgc gtcaatttct gcctgatttt
     7021 ggaagagacc atctgtttta taaccataga aataaggaaa tacttcaccg tttttaccct
     7081 ttacatagct acctacacct gatgctccgg cattttcgta aatttgttct ccggttgcat
     7141 taccaagttt gatcaacttg tttttcagat aggaagcgtt agccgaaacc gagtacttga
     7201 aatcaccggc tgtttgtttc catcctgttt caaattcaag tccccaattc tgcatatcac
     7261 ctacgttagc aatgggagaa ccttgtccta cataagatgg gataggttgt tccatcaaca
     7321 taccgttagt gatttttttg aaataatcaa aactgaaagt gaaagcactt ttaaacagat
     7381 aagtttcgaa acctatatcg gtttgttgcg attcttccca ttttacgtcg ggatttggta
     7441 tagatgctgg tgaacttcca tactgcatta cacctcctgt ttcacccact ttggtcgggt
     7501 ctccgttatt cactttatat ccacctccga aatagtagtt ctgtccacca tccatcagag
     7561 aagtgtaacg gaatgctcca attcgttcat taccgttttt accccagctg aaacgaagtt
     7621 tcaacgcgtc aatccatttt ggtctgtcct gcaggaattc ttcgttggtc aaattccatc
     7681 caagtgaaaa tgcagggaat gtagcccatt tgtgattcgg tccaaagttg gacgaaccgt
     7741 cacggcgaac cgtagcctga aggatataac gttcggcata attgtaatct aaacgagcaa
     7801 aataggatgc cagagttgcc tgattataac cacctgtacc accccaaacc cgttcgagtt
     7861 tacgatcagc aatggctgag tttatgttgg ctttcagcgg atcagtttcg agcaattgct
     7921 ggtcatttcc tcccaggctg cgataggtgt atttttgagc cgattgtccg agtacagcag
     7981 caacattgtg tttgccacca aaagttttat tgtatgaaag tgtattttca acctgccatt
     8041 tgaaacctct gttcatttca ctctgaactg tacttacatc tacagttttt ccctgagtag
     8101 caagaaaata tgggaaagta tagctatcat agccccagaa agccaggtcg acaccataac
     8161 tgctcttaaa tttgagttct ggcaggacac ttaattcacc ccaaaaggtg gaaacgattt
     8221 tatcgctgtt gttcaatcca ctgcttggct gattgagcat ggcaaccggg ttagctattt
     8281 cctgaaatcc cgagggagga atggaaaata cccgtccgtc tttgtctttt acagcatttg
     8341 gatatttggc aaggatagct gctgcagttt cttcgtcggc atatacaggt accaacggag
     8401 agaaagtcaa tgcactacca aggatggagc catattccga gttggcatca atcgaagaac
     8461 ttttgtcacg ggaatagccc aggttaacac ctatgcgtaa tttattcaga aaatcgcggc
     8521 ttttttcttc gaaaatggta tgtgtcgaat tggaacgaac actccagcga ttgtaatttg
     8581 atttgccgaa gtcaccaccg acaataccct gctggtcgaa atatcctaat gacaggaaat
     8641 attgcgatct ttcggtacct ccggaaacgc tcaactgatg attttttatc ggggcattgt
     8701 agttgaaggt ttcatcctgc cagtcggttc ctgttccggc gttcattacc tgttcgttgt
     8761 tatatcttgg gagacctcca tcgttaataa gcccttcgtt cattatgacc atatattctt
     8821 gagcattaag aaccgatttc tttttccagg gattttgcca tccataactt acattgtagt
     8881 taatcttggt tttaccggcg actccgttct tggttgttac caatactact ccgttagcag
     8941 cacgtgctcc gtaaatagca gccgatgaag catctttaag gatttccacc gattggatat
     9001 ccaccggatt gaggtaatta ataccaccgt caaccggcat accgtcaacg atataaagcg
     9061 gctcactatt gtttacggta ccaataccac gaatacgtac tttagaatcg gatcccggtt
     9121 gtccggaact ttgtgtaatc tgaacaccgg aaactttacc tttcagtaca tcttccacgc
     9181 gcgatggctt tgtgttgttc agttgttcag ctgttacacg gctgatagca gcagtaacca
     9241 cacttttctt ctgtacgccg taaccgataa ccaccagctc attcagactt tttgtgtctt
     9301 ccttcagggt tatattcatt gttgacggac tgttaacagt aaattgctgt tcgacatatc
     9361 cgatataagt tacatgtaca acagatccgg caggaacaga aagactgaaa ctgcctgtag
     9421 cgttgcttac agtaccgatt gaactgttgg gaatggaaat gttggcaccg ataatcggat
     9481 cacctttctc gtcgataact ttcccctgaa agagaacagt tggttgattt tgctgagatg
     9541 tcagttttgg agcctgacta ccggcaatgt agattttttt gtccctgatt tcataactca
     9601 ggtttcgtga agataacaat tttgtcatta catccgaaag agaggcattg ctaacattaa
     9661 tgttaacaat ctcattcaga ttaacgacct ctttgctgta agctaaagta tagcctgtct
     9721 gacggctgat actttccaga acttgtttta aagtttgctg acttgaattt aaacttacct
     9781 tctgtgcaaa gcccatattg ctcaggccta taaacatcag tgtaaataaa ataaaagtcc
     9841 gccttttaaa aagttgtttt ctcatggtat aacttttttt taagtgaaac aataatattg
     9901 aatttaaaat attcccggat aattgaaaaa tctataaatg taatataaca gacaacaaca
     9961 gttaaatatt accgcattgc ttatattcaa gcatttatga aagtctgatt taagatttca
    10021 acagtgtcga tcaattgatc gtatgtcatc aacaaacaat agacacacga taagaaaaaa
    10081 agtatgagtt aaaatcagaa tttattttaa atatatattc acgctgttga atttggtcgt
    10141 cgagaattta acgggagaga ttttctggag ttctttaatt atgttctcca gcgaagtttg
    10201 gttggttgta agggtgtatg tataggtcag cgctttagga tcctgaacat taaagtcgac
    10261 attgaatttc cggttgagaa ctttaattac atcagccagc ggagtatcgt gaaacaatat
    10321 gatattgtct ttccatacgg acacttcata gggtctgtcg atataatcaa tggtgcattt
    10381 cccggtcagt acatcataga cagcctgctg tccgggcaat atctgataac tatcgcaggg
    10441 agtgttgaaa atgatatttc ctttatccag caatattttc atttcccgtt cctcctgata
    10501 ggcatgaaca ttgaatgaag taccaagcac tgtgacatta gtattattga tatttaccac
    10561 aaatggtcgt cgcttattgc tggcgatatt gaaataacct tcgccctgaa gaaatatttc
    10621 acgttttgac aaaccgaact tacgggggaa tcgtaatcgg gtatcagcat tcagataagc
    10681 ctcgctccca tcctgaaaaa caatatgctc ctgtttacct ttggggacat atacttcagc
    10741 atatactgtt ttgccgaata agttaacctg attgttgagc tgaaaagtga ttccgagaat
    10801 cataattacc gggatgagca ctgctgctgc ctgccagtaa atcttgcgca tccttttatg
    10861 tctgatttgc ctgtttattt cggaaagtat gttagctgac tgaacagaag acagggtaat
    10921 gtatttgttc gggttttcat ccaggacacc tgcttcgcag tccagcaggt gagacaggta
    10981 tgcctgacct tcaactgttt cagaaaacca catagctact tcacgggctt cctttttgct
    11041 ggctatacct tctataacct tatcaatggt ctctttttcg aacggatgca tattattttc
    11101 tttatattac cttgacacaa gaatgaaaaa ataatgtgag ttctatatgt taattattta
    11161 taaaaacatc aattttattt ttcggaagta gtctctgagc atctttacag actcctgata
    11221 gtgcgatttg actgtattta ccgatattcc catcgcatct gcaatctcct gattactttt
    11281 attttcatca attttcatct tgcagatttc cattttttgg gatggcagat tattaatgcc
    11341 ttcataaaga aagttgctga gctgattatc ttcgagcatt ttcagaatat cgctgtcgtc
    11401 cgaaatttca atctgattat tctgataatt caacgaaatc atttccttat tatctcttat
    11461 ctgattaaga ataaagttct ttgtcatcgt ataaagatag tttttaagat tgatttctat
    11521 ttcgataaac gggcttgaaa cccatagctt tacaaacacc tgttgcactg catcacccga
    11581 catctcggta tctttcagat atcggtaagc aaggttgtac agatattgat gatacttata
    11641 atatacaatt gtaaaagcct ctttatcctt tttttttatt aactggaata aagcggcatc
    11701 ttcattgtct gcatattttg agaattgggt tcgcatagtt aaaccgtaaa ttttgttctc
    11761 tttatcagca aaagtaatga ttatttcaaa ttgattacgc tacaagaatg tatttttata
    11821 atattatcaa ttacagcaca cctgaagcca ttaaaaaatt aaaatatgat attctcaaat
    11881 accttcataa aataattccc ggaagtattg gtgatcattt ctcaataatt aagttccgga
    11941 caccaccttc gtgaactata atcaaattga aggaaataat ttcctttatt aatcccgttg
    12001 aaactttcgt gattgctggt ggagaatgaa aaagcttata ttttattcag atagaataag
    12061 atataaaaaa aagaaataaa gaccttttta tcatttgtgg aggagaatac agaactaaaa
    12121 gtcattacca ttttttagtt cccttttgat aaataagtat ttttcctgct catcatgcag
    12181 gatatctggc tggataattt cgagcaattc aagtgtctcg tacagaaaca gagcatcctg
    12241 aatgcttatg gaatttttta tttgagagaa ataaccatac ttaatggcat acttattaat
    12301 aaacgataaa aatctcccga catatgtttt cttttcatct aactctcttt ccgcctccat
    12361 tgatatgatc aaatctatat tttctctgtc aataacgatt tttatggatt tattcttctt
    12421 catataattc atggcaaatg tcgtggtttc caacacttct atctttctct tatcaggtgg
    12481 ccattgagat gcattttcac acgaccatgt ttttctcggg aaatgaaact cagatttttc
    12541 attgagtctt accatcagga atgtttcaga taattgtttc tttactactt cctgaaattc
    12601 cggtatctgt tttttcgaaa tttccgaagt gttatccggg ttatctttcc ctgtcaaaat
    12661 aacgccacct tcttctgaat aaagaaattt tagcaaacga gccaatctga gcctttcttt
    12721 ataactggca tcttcacggt cgccaatcag gaataaatcc cataaaataa gataataaag
    12781 cccttcaagt cttatatcgg gcttatctat taattcaatt tgctggagta taatcttttc
    12841 acgatagtta gtagcaggat aatgctctgc cttaatcaaa tagttttcct tgttgaatat
    12901 tggagcactc atagcttact tcctgaaaat tatattattc tgattactta taataatgta
    12961 ttttagttat aacaaataat ttcaatttaa tgtttcatgc tttgaatgcc aacaataatc
    13021 aataaactat ataaattact aaaaaacaat ttacaactcc gaaataagac tctaaaaaaa
    13081 ctgccggtca tcaaaataga ttgaagccgg cagttaaaat tattttaaat tacattagtt
    13141 catttagtta cctatctcgg ataaaaattt aatccgcacc agtcttactt ctgtttcact
    13201 gaaatcattt tcaccgagtt cgtcgagtgc agtacttatt ttatcggttt cagcagaatt
    13261 gaaatattcg aatatttcat ccacacgatc aggatccatt acctcatcga gaaaatagtc
    13321 gatatttatt ttggtacccg aatacacaat ggcttctatc tcgtccagta attcgcttat
    13381 atccagccct ttggaaagtg ctatatcatc aagtgcaatt ttccggtcta ttgactgtat
    13441 gattgacact ttgagttttg atttattggc gacagttctt acgcgcaaat cttcaggacg
    13501 tatgatttca ttttccttga catgttcgcg tattactttc aagaattctt ctccgtaacg
    13561 ttttgcttta ccggctccta cacccgggat attttgcagt tcatccatcg aaatggggta
    13621 actggtagcc atagcttcga gcgatggatc ctgaaagata acaaaaggcg gtacttccag
    13681 tttctttgaa agtttcttac gaagatcttt cagaatagag aaaagcacat catcggcagc
    13741 acaggtacca ccggcatttc ggctactcat atcatcttcg tcatcctcaa attcagtgtc
    13801 tttcacaatc gggaacgact tcggattttt cagatattct tttcctgaag cagtcatttt
    13861 caagactcca taggtttcaa tatcttttga gatataacct gctatcatgg cctgccggat
    13921 gactgcgtgc agcagttttt catcctcgtc ggatgctgaa ccaaaatttt cgaggtcaac
    13981 atgctgataa gattttacgt caggtgattc atttccttta aggacatcaa caatatgttc
    14041 agctttgaat ttttctttaa gttgctgaac ggtctccaat actaatacta ataattcttt
    14101 tccttccaca aattttcttg gtttcataca gttatcacaa ctgccgcaat tttcgagggt
    14161 atattcttct ccaaaataat gcagcaacaa ttttcttcta caaacagaag attcagcata
    14221 agcctgcgtt tcgagcaaaa gttgatttcc aatctcctgt tcggccacag gttttccctg
    14281 cataaattta cgcagtttat ccaggtcttt ataagcataa aatgcaatgc attgaccttc
    14341 accgccatca cgtccaccac gtcctgtttc ctgatagtaa ccttccagac tttttggaat
    14401 atcgtaatga attacataac gtacatccgg cttgtcaatc cccatcccaa atgcgattgt
    14461 agccacaatg acatccgctt tctccatcag gaacttatcc tggttttcgc tgcgttgcaa
    14521 cgagtccatg ccggcatgat aggctaatgc agaaatacca tttacctgca aagtttctgc
    14581 aagctcttca actttctttc gactcagaca atatattata cctgatttac ctgcttgaga
    14641 gcggatgtat ttaataatat ctttatctac atctttggtc ttagatctta cttcgtaata
    14701 caggtttggt cggttaaagg acgacttgaa tacagttgca ttaagcatgt tcagattttt
    14761 ctgtatatca tgctgtactt taggagtagc ggtggcggta agcgcgatca aaggagcgac
    14821 tcctatttca ttgataatcg gtcttatgcg gcggtattca ggtctgaaat catgtcccca
    14881 ttccgagata caatgcgctt cgtcaatagc ataaaatgaa atttttacct gacgaagaaa
    14941 ttcaatattc tcctctttgg ttaatgactc cggtgctaca taaagcaatt ttgttttccc
    15001 tgaaattaca tcttctttca caacatcaat agccgcacgc gtgagtgacg aattcagaaa
    15061 atgcgcaacg ccatcttctt cgctgaaatt tcgcattgca tcaacctgat ttttcatcag
    15121 ggcaatgagc ggagaaatta caatggcagt tccctccatt aaaagtgagg gtaattgata
    15181 acacaaggat ttgccacctc ctgttggcat cagaacaaac gtgtcctttc catcaagaac
    15241 attttgaata attgcctcct gattgccttt gaatgtatca aaaccaaagt attctttcag
    15301 atatgatgtt aactccgagt gctttgtcat agtaattact cttcagagaa tgaaaacgaa
    15361 attacagtta ttttttagat tacacaaata atttttatca gataaaatat attttctaat
    15421 cgcagatgaa ataacaaaaa tataaacaga ttttaaaaaa acaagaggtt tgaaataaaa
    15481 aatcaggata tttgtaattt aaaaacataa caccctgatt ttctattctt tatcaatagt
    15541 aataatttat aatattctac gaatgtcagg ccgttttcaa acggtgagca ttcgccttat
    15601 ccattttagt ccgggcataa tcgatggtta ccacccattt tttctctttt ttggtaggca
    15661 tctcaaacat ttggtctatc atgatggttt cagtgattga tctcaacccg cgagctccca
    15721 gtttgaattc gattgcctta tctacaatat aatcaagcac ttccggttca aaaatgagat
    15781 ccacgccatc catcttaaac agttttacat actgcttaat aattgaattt ttcggttctg
    15841 tgaggatatt ccgcaatgca ctccgatcca aaggttccag gtaggtcagt ataggaagac
    15901 gtccgataat ttcgggtatc agtccgaatg atttcaaatc ctgcggggca atatattgaa
    15961 gcagattggc cctgtcaacc atttcgtttt caatagatgc accataacct acaacacgtg
    16021 tgttgaggcg gttagctatt tttcgttcga ttccgtcgaa tgctccaccg cagataaaga
    16081 ggatattctg ggtattgaca gggataaatt tttgttcggg atgttttcgt ccaccctgtg
    16141 gcggaacatt taccactgaa ccttcgagca gtttcagcaa accctgctga acaccttcgc
    16201 cactcacatc gcgagtaata ctcggattgt cactcttacg ggctatttta tctatttcgt
    16261 ctatgaacac aatacctctt tccgcttcct tcacattgta atcggctacc tgcaggaggc
    16321 gtgtgagaat gctttcaata tcttcgccta cataaccggc ttcggtgaga acggttgcat
    16381 cgacaatggt gaatggtacg tgcagtttac gtgcaatagt ttttgcaagt aaagtttttc
    16441 cggtaccggt ataaccaacc atgatgatat tggatttttc aatctccaca tcgtcgtttg
    16501 atttttcctg caatacacgc ttgtaatgat tgtaaacagc gaccgacaag tattttttcg
    16561 catcatcctg tccgatgacg tactggtcca gataattttt aatttcggca ggcttcggca
    16621 atttttcttt gtcaagtgtc gaagccatat cattgcccat ttttgcatca cgtactattg
    16681 cagcagcctg ttcggtgcat tcgttacaaa tctgtgcgcc atcaagtccg gtaattaaaa
    16741 agcctacctg tgactcgggt cttccgcaga agctgcaatg tttactgttt tttgccataa
    16801 attatttttt ttcttttatc aacactctgt caatcattcc atattccagg gcttcctgtg
    16861 acgtcatcca aaaatcgcga tcgctgtctt tttcaatctg ctcataggtt ttacccgaat
    16921 gatcagcaat aatctgatac agttcttttt tgagtttcag catttcgcgc aggttgattt
    16981 ccatatcggc agccactccc tgagctccgc cacttggctg atgaatcatg atacgtgaat
    17041 gtttcaaagc cgaacgtttt ccattttttc cggctaccag caacactgcg cccatagatg
    17101 ctgccattcc tgtacagatg gttgccacat cggacgaaat aaactgcatg gtatcgtaaa
    17161 tgcccagtcc ggcataaaca gagccacccg gagtgttcag atagattgaa atatctttgc
    17221 cgggatcggc cgaatcaaga tacaagagct gagcctgaat gacgttagcg gtatagtcat
    17281 ctacctgagt ccccagaaaa ataatccggt ccatcatcag ccgggagaat acatccatct
    17341 gtgtcacatt caactgacgt tcttccagaa tggaaggtga aatataacca ttgttcatta
    17401 ccttaaaata ctcatcgagc gataagccat tcatacccag gtgtttggta gcgtatttac
    17461 gaaaatcgtt gttgttcatg tttttttgtt tttaattatt actcatttta ttctgagaaa
    17521 tatataacta aaatcctgcc aaaattgttt ttctgccaaa atggcagtat ttaaaaagca
    17581 aagaagattt ttaaatgact attcagcaaa gataactgat ttttggtgaa taaaatgatt
    17641 taaaaacctt ctccgcaata ttaggaaatt ttaattttcg cctgtttctt gaaacatctt
    17701 attaaaatct tcaactgata tttctttttc gttcagctta acagtgcctt ttatgtagtc
    17761 aagcactttt tcttccatgg ctctgtccac aaagtttttc actgtttctt ccttcttcat
    17821 caggtctttg gcatagtttt ctataatcga atcctcaaga ccaaccattc cgtactgagc
    17881 aaattgggat tttgccactt ttttggcata ttcctgcaca tcggctgttt ccactttgat
    17941 tccattagca tcagcaattt tgtcttttat caggtgccag gtcaggtctt taatcattcg
    18001 cggataatct tcttcgagcg attcgctggt catattttcg ttggaatata acagccagcg
    18061 tttgaggaac ttatccggga atttcaaatc gttgtatttt gccgataaag cagcctgcgc
    18121 atcaattcca aatttgtaat cgctgtcggc ggtaaggttt tccttgatgc tttcagcaat
    18181 tttttgacgg aattcatttt cggaagttac tgcgccttcg ccaaatactt tgtcgaacaa
    18241 ttcctgattg atttcgcctt cttcataacg ggtaatgcct tcaatctgca tcataaagtc
    18301 ggaagtgatt tttttggctt cgtctttcga tattttaagc atcgagctga tttctacttc
    18361 attttcgtag gcaacctgcg gattaaacac aaccttatcg ccgatctttt ttccaacgaa
    18421 caatgctttc tgatctgcat ttttcatata agcaggtgtc aaaacggcat ccttcacttt
    18481 cagaccgtct tcattgactt taccattagc catttcgagc aattcacctt tcaccatgtc
    18541 gttttcttcc actacatcta cctgagtata ttttccgaaa cgcgaagtgt atgatttaac
    18601 ctgattatca atcatttgct cagaaacttt tatgttataa taagtgattt tgtcgctttt
    18661 cgacagatca acatcaaact ggggtgctaa tccaatttca aaatcaaaat caaaatcttc
    18721 gctgttttca aaatcaacat cgtgatgatt ttctacagga agtggctcgc caaggaattt
    18781 gatattattc tctttcacat acccgaataa accatcggaa actaatttat tgacttcttc
    18841 tgccacgatg gctttgccat acagtttttt tatcaggcct accggcacat tacccggccg
    18901 aaatcccgga acattcgctt ttttacgata attacgaatg tatttttcta cattttcgtt
    18961 gtaatcgtct ttggtaacac ttacggttat gactgctttg gtttgatcaa gatcttttct
    19021 gacgaatttc atatactgtt aattattagc acattgtgca attgtttatt ttcaaacata
    19081 tttggggtgc aaaggtacaa aaagttaata taattcatgc tttttttcaa caaaaaaaga
    19141 taaaattgaa tctgttatct tttatttttt ttcttcgaac aggacagtag tactccggaa
    19201 tgatattgca actaaccgat ggtgcttctt tatattgaat tattgttgac aataccggaa
    19261 tggagcaaac ttttacagag taccagaaat tattccattt tatgaaaaaa gaagctgttt
    19321 ccttgtcaga tgaaatatcc tgaccaagaa aacagctgaa aaatatgttt gaatgaattt
    19381 cgctatttat aaattggccc gaagtttttg caagcccggt aatcagcgcc ttctttatcc
    19441 attccgaatg cattccaggc tgatggtctg aaaacttcgt cctcctcaac attgtgcatg
    19501 caaaccggaa tacgaagcat agccgcaagt gtaatcaaat cttttccaat gtgtccatag
    19561 ctgatggtac cgtgatttgc tccccagcaa ctcatcacag aatatacatc tttaaacggt
    19621 gctgaacctg tgagacgcgg tgcaaaccag gtggtcggcc aggttttatc tgttcgctgg
    19681 ttgagtgcat cgtgaatttc tgtttctaat tctactgtcc aaccttcggc aatctgcaat
    19741 accggtccca ggcctttcac caaatttaag cgagacatgg taaccggcat tccgcctttg
    19801 gagaggaagt tagatgaaaa accaccgcca cggaaatagt tgcggtcggc cggataccag
    19861 gttgttgcac ccaaacaagc ctccacttca ttttcggtga tttcccagaa aggtttgagc
    19921 gccggttctc catctctgga ttgttgcccc gttccgtcga gtgtggttga cccggagttg
    19981 atcaggtgaa taattccatg ctgagccatt ccggtcagtt cccgacctgt cacacgctta
    20041 acagcttcgg gactccagta ggtacgaaca tccgaaaaaa gctgagatgt attggtcagt
    20101 aggtgtccga acagcatggc tactccgttg caggcatcat tctcagtagc aagtacgaat
    20161 gcttcacgga tgccattcca atcgaaagag ctgttaagca gcgcttcgga gaagtcgcca
    20221 ttcggcagga agtcggtcca ctgacgctgt ccctgaaatc cggccagtat tgcgttatga
    20281 ccaatggctt cttccttaaa accatttttc tgtaattcgg gatttccctg catcagatca
    20341 cgcatgacca gagtcatttt caccacaaac tcccagtctg cttctttttc ggttcttgtt
    20401 ttctggtttt cggcgaggtt gtaatcgaca ccttcattca ccttgcaata tttttcagtc
    20461 cacgccattg ccttttcata ctctttgtga tcgtaaatgc ccagtgaaat tctccggtga
    20521 atttcggtca tatcaaccac ttcgttgcgc atgccgaggt attcctggaa gaaatcggga
    20581 ttgacgatag agcctgctat acccatcgaa acgcttccga ttgaaagata agattttcca
    20641 cgcatggtag ccacagcctg agcgctgcgt gcaaaccgca gtattttcac ggcgacatct
    20701 tccggaatgg aattatcgcc ggcatcctgt acatcccggc cataaatacc gaatgcggga
    20761 agacctttct gaacatgtgc agcaagtaca gcagccagat acactgctcc gggacgttcg
    20821 gttccgttga aaccccatac cgctttcggg taatatggat tcatatccat ggtttctgca
    20881 ccatagcacc agcaagaggt aaccgtaatg gttgaaccta cgccttcgcg ttcaaatttc
    20941 tctgcacagg cagcactttc gcctacacga ccgatggtgg tatctgcaat aacacattcc
    21001 acaggacttc cgtcaccgtt tttcagattt gaactgatca gagcggctac tgcactggcc
    21061 aggttcattg ttttttcttc aagactttct ctcacaccac cctgacgtcc gtcgatggtt
    21121 ggcctaattc cgatttttgg atattttttc atcgtattat ttttgttaaa ttactgttat
    21181 cgtatatttt aatgtttatg ttttcgacta cgcacacttt actgtgctgt actgtgctgt
    21241 tgtttttata aaaaaatata taacattctg atttcttata ttttagcaac ctgttcctga
    21301 ctttctgttc tttgttcgga ataaaaaata ttcagaacaa agatatataa cacaattcaa
    21361 aaaaataaaa gatattaata tgttgttcag catgtttcag cacaaaaact acagttcagt
    21421 ttggttaaaa tacaatcaga cctcaacttt gatttcaccg gaaagagaca tacgagatat
    21481 cacttttttt tgtaattttg tcgggcttca gagaaatggg aataaatgaa tatttcacat
    21541 catatttgaa agataaaaac cattgaaaac caacaattaa tcattcgaaa ttaaaacact
    21601 gtcaatcatg aaaaaattaa ttttatttac aagcctgttg ttaagtctca cgacattttc
    21661 tgttttttca cagggaatac aatttgaaga aaatcacgat ctgaacgctg ccttagctaa
    21721 agcaaaagca gaaaacaaac tgatttttat cgatgcgtat gccgtatggt gcggaccctg
    21781 caaagtgatg gcacgtgata ttttcccgct caaagaagtc ggcgattatt tcaacgataa
    21841 atttgtaaac ctgaaactgg acatggaagc gactgaaaat gtggagatag ccaaaaaata
    21901 cgaagtaaaa gcatatccca catatctgtt tctgaatgcc gacggtgagg ttgtacataa
    21961 aggattggga agcatgccgg ctgaaaagtt cattgaagta gcaaaggcag ctgcggacac
    22021 cgaaaataac ttcagcgctc tctcgaaaaa aattaaaaac ggagaccgca gccttgaaac
    22081 catcaacaag tatcttgctc aaaatcccta tgatgccgga aacggcgatt tgttggacga
    22141 atatttcaaa acactttccg aaaatcagat caacacaaag gagaactggg aattgttcag
    22201 caacaatatc gataagattt catctccgtg gtttcagtat ttcatcgcta acagagaaaa
    22261 attctctgct cttgtcggaa aagataaaac ggatgcaaaa attgcaaaaa tcatgataac
    22321 tgcttacaat caggacccgt cgagcgaaga aaaaattaaa agcatagatc cgttgctgtt
    22381 tgaagatgtt aaggccaaag taaatctttc gaaagcatac ggacagttcc gcaaagcaaa
    22441 caatatggaa agctggaaaa gcttcatcaa ccaggctgta acttattata aagattgtaa
    22501 ggaagccaac caactgaatg aaatagcctg gatggtgttt aaaaacaaca gtaagttttc
    22561 ggataaagag ttattaaagc agggacttaa gtgggccagt aaagcagtaa tcctggaacc
    22621 tgggaatgat gcatttcttg acacctatgc caacctgctt tatgcatcag gaaagaaaaa
    22681 ggaggctatc aaaactgaaa caaatgcatt gaaaattgca gagaaagccg gcaatactga
    22741 agctgtggaa aattacaaga aagtgattga aaattttaaa aacaaataat atagtaaatc
    22801 ggattctgat aaatttcata acccggaacc cttaacaaaa gaaattatgg ctaaaccatt
    22861 tatttatcag gaagcatttc ctgttggaaa agaaaaaacc gaatattacc tgctgacaaa
    22921 agattatgtt tcggtatcgg agtttgaagg acaagaaata ctcaaagtac aaccagaagg
    22981 tcttaccgcc cttgcacgca cctgtatgcg cgactgttct ttcctgctgc gtcctgaaca
    23041 tcagaaacaa gttgcaaaaa ttttggccga tcccgaagca agcgaaaacg acaaattcgt
    23101 agctgtaacg atgctccgca atgctgaaat tgccgccaaa ggtgtgcttc ctttctgtca
    23161 ggacacaggt actgccaccg tttatgcaaa aaaagggaac aatgtatgga caaacggtga
    23221 cgatgaagaa gcactttcga aaggtatcta cgaaacttat accaacgaaa acctgcgtta
    23281 ttcacaaaac gccgcgctca acatgtatga tgaagtgaat actggtacaa atcttcctgc
    23341 acagattgac ctcgttgcaa gtcacggtaa tgaatataaa tttttgttta ttgccaaagg
    23401 tggcggatca gccaataaat cttatctgtt tcaggaaaca aaagcgctgc ttaatcctgt
    23461 ttcactggaa aaatttctgg tagaaaaaat gaaaacgctg ggaacggctg cttgtcctcc
    23521 ttaccacatt gcattcgtta tcggcggcac atcagccgac gcttgtatga aaaccgtaaa
    23581 actggcttcg accaagtatt acgatgaact gccgacaacc ggtaatgaac tcggtcaggc
    23641 tttccgcgat gtggaaatgg aagaaaaagt gttgttagca gcccagaaag ccggctatgg
    23701 agctcagttc ggcggtaaat actttgcaca cgatatccgc atcattcgtc tttcacgtca
    23761 cggtgcttca tgctttgtag gcatggcagt ttcgtgttct gccgaccgca acatcaaagg
    23821 gaaaatcaat aaagaaggaa tttgggtgga aaaactggaa gacaatccgg gtcagtacat
    23881 tccggaagaa taccgtcagc agggcgaagg caatgctgtg aaaattgacc tgaaccaacc
    23941 tatgccgcaa attctgaaaa ctcttagcca gtttgcggta tctacgcggc tttcgcttac
    24001 tggtacaatt atcgttggcc gggacattgc tcacgccaag ttgaaagaac gcatagacgc
    24061 aggtttcggc ttgccacaat acatgaaaga tcaccccatt tactatgccg gtcctgccaa
    24121 aacaccagcg ggaatgcctt ctggatcatt cggaccaaca actgctggac gcatggacag
    24181 ttatgtggac ttgttccagt cggagggtgg cagtatggtg atgatagcaa aaggcaaccg
    24241 cagccagcaa gttactgacg cctgcaagaa atacggagga ttctatctcg gatctattgg
    24301 cggtcccgct gctattttgg cacagaacag catcaaaaaa gttgaatgtc tcgaatatcc
    24361 cgaacttggc atggaagcca tctggaaaat tgaggtagtg gatttccctg catttatcct
    24421 tgtagatgat aaaggcaatg atttctttca aaaaataaaa caacactcca tgtgctgaaa
    24481 ttattaagac attaacagag aaaaccgttg agcatgaaaa ttcaacggtt tttttattga
    24541 caatcagcaa tctattttgt aaatttgtaa acgattgagg aataaaacct tattatccat
    24601 tcaataacac gtacagcaat gaaaaacagg ataaccatgg gaaatgacga gtttatactc
    24661 tacatccgca agaactacaa ctgttcgctt acaaacgacc ggctaggtag aatgatagga
    24721 aaatggatac tggaaaactc gaaaggcatc agcaaaagaa atgatattga gcatgatatc
    24781 ccctgcctct ggcaacaaaa catccgggca aaaaaaacct atggaacccg cctgccccgc
    24841 acagcctctc aaatcacttt caaccgcaat atcctgccaa aactttatga ctatctggat
    24901 tttcttggaa aaaaatacgt cagcaaccaa ccctggaatg agtggaaaaa aagagaggac
    24961 gaaaccgaag acgcaggcat gctggaactt aatttctgaa atatattctg acatcaggaa
    25021 cagtataata aataaaa
//
LOCUS       DAOPYB010000026        24930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_1991_length_24930_cov_9.406995, whole genome shotgun sequence.
ACCESSION   DAOPYB010000026 DAOPYB010000000
VERSION     DAOPYB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 24930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24930
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1991_length_24930_cov_9.406995"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PKH58_03575"
     CDS             complement(<1..130)
                     /locus_tag="PKH58_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNX88137.1"
                     /translation="MDTKKNFVIDTNVILHDYNCLHNFEENDVHIPIVVLEELDHFK"
     gene            complement(382..720)
                     /locus_tag="PKH58_03580"
     CDS             complement(382..720)
                     /locus_tag="PKH58_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX88138.1"
                     /translation="MRKFTLILTFLLCGLTAIQSQKNSTLFDKNTTETNNTQSNKTSD
                     NEIVPRFTISGKTLNVTNLEIGVQIDIYSALGAKVLTVVYNGTNISLVNLNKGIYIVR
                     AGKFTQKIML"
     gene            984..2630
                     /locus_tag="PKH58_03585"
     CDS             984..2630
                     /locus_tag="PKH58_03585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX88139.1"
                     /translation="MKRTEPEYIFETSWEVCNMVGGIYTVLSTRAETLYKKFNDKLIF
                     IGPDIWKDTESPYFEPSDTLHCEWKNNFTEKTGLNVRCGRWKIPGNPLTVLVDFTPLL
                     NRKNEIFGHIWDKFGVDSLHGYGDYDESSMFGYATGMIIESFYRFYNLSAKNNVIAHF
                     NEWMTTFGAFHIKEVVPEIATVFTTHATSIGRSIAGNHKPLYDYLSEYNGDQMAFELN
                     MVAKHSTEKTAAHVVDCFTTVSNITSKECKQLLEKAPHIITPNGFDDSIVPTGKDFNV
                     KRKAARSRLKKVAETLLGYKLKNNVLFINTAGRYEYKNKGIDTFIESLKSLTNHPELN
                     REVVAFIMVPAHISGPRADLAAGLNGSELAIDYWNKHTTHELYDYQYDHVISALRWFH
                     FTNLQNEKVKVIFVPSYLNGNDGIFNMTYLDLLIGMDVSIFPSYYEPWGYTPLESVAF
                     SVPTITSDKSGFGQWVKSNYDTRHGVAVVHRSDYNHTEMCEEIGDILAKFTNYNSDKT
                     DDVRKKALATSKRALWSVFIDYYYQTYEIALKNRNHRLKH"
     gene            2817..5378
                     /gene="glgP"
                     /locus_tag="PKH58_03590"
     CDS             2817..5378
                     /gene="glgP"
                     /locus_tag="PKH58_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNX88140.1"
                     /translation="MKLKVNSTNDIPWKNIIIKSKLPENLNKLEEMAQNIWWSWDSEA
                     KKLFREIDRPVWKEAASNPVQLLNLLNYDKLDQLANNPEFIEKVDNVYAKFKAYMNEP
                     TNTNRPSIAYFSMEYGLNEVLKIYSGGLGVLAGDYLKEASDSNVDMTAVGFLYRYGYF
                     TQSLSVDGEQISVYEPQNFNTLPIKQVMDENNQPLEVEVPYPGRTVYAHVWKVSVGRV
                     SLYLLDTDYDKNSEFDRSITHQLYGGDWENRLKQEIMLGIGGILALKKLGIKKQIYHC
                     NEGHAALINVQRLVDLIHDENLTFNQALEVVRASSIYTVHTPVPAGHDSFDEGLFGRY
                     MSEYPGKLQISWNELMDMGRENPGDNNEKFNMSVFACNTNQEVNGVSWLHGEVSKKMF
                     APIWKGYFPEESHVSYVTNGVHLPSWTSSEWKALYEKYFTKAFYEDQSNLKIWEKIYQ
                     VPDQEIWNLRLSLKNKLVEYIRMQFQENWIKNQGDPSRIVSILDSINPNALIIGFGRR
                     FATYKRAHLLFTDLERLEKIVNDPIHPVQFLYTGKAHPADGAGKGLIKRIVEISHMPQ
                     FLGKIIFLENYDMRLAKRLISGVDIWLNTPTRPQEASGTSGEKAQMNGVLNFSVLDGW
                     WYEGYKKNAGWALTEKRTFQNQEFQDKLDAITIYNILENEIIPLYFAKNTKGYSPEWI
                     QYIKNSIAQITPRFTTKRMMDDYFERFYIKQAKRSGLLMANNYKVAKEIANWKETLSA
                     GWNNIEVISVESSEEFLNNPQVGENYETKVIIDTKMNEKSVGVEMVTIYTDKNNNDHF
                     FKATELNLVKTEGSRMYFSLTFKLQQAGVYKTSYRIFPKNELLPHRQDFAYVRWF"
     gene            complement(5459..6484)
                     /gene="tdh"
                     /locus_tag="PKH58_03595"
     CDS             complement(5459..6484)
                     /gene="tdh"
                     /locus_tag="PKH58_03595"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNX88141.1"
                     /translation="MKALVKLRPEKGIWMENVPVPEIGINDVLIKIKKTAICGTDLHI
                     YKWDDWSQKTIKTPMTIGHEYVGVIVDKGAGVKNVKIGDRVTGEGHIACGHCRNCRRG
                     KLHVCENTIGVGVNRDGAFAEYLSLPESNVVHLDERISDEIASIMDPFGNATHTALSF
                     PVLGEDVLITGAGLIGTLATAICKFGGARNIVVTDLSEYRLNIAKKMGATCTVNGLKG
                     ETVEGARKELKLRGFDVGLEMSGAPAAFREMIDNMYNGSKIALLGILPNSTTVDWNEI
                     IFKALTLKGIYGREMWETWYKMQQMLLSGLDLTPVITHRFPINEFQKGFDVMESGQCG
                     KVILDWE"
     gene            complement(6545..7735)
                     /gene="kbl"
                     /locus_tag="PKH58_03600"
     CDS             complement(6545..7735)
                     /gene="kbl"
                     /locus_tag="PKH58_03600"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNX88142.1"
                     /translation="MYQDYKSHLQVELSGIKEAGLYKNERIIVSPQGAVIRIADGKEV
                     LNFCANNYLGLANNQLLKDAAKQAIDTHGYGVSSVRFICGTQDLHKKLEASIARFFGT
                     EDTILYAACFDANGGVFEPLLTEDDAIISDALNHASIIDGVRLCKAQRYRYANADMTD
                     LEDQLKKAQNQRFRLIVTDGVFSMDGNVAPLDKIYELAEKYNAMVMIDESHSAGVVGR
                     TGRGVTERYDLRGKIEIITGTLGKAFGGAIGGFTTGKKEIIELLRQRSRPYLFSNSIP
                     PMVAAAGLKMFELIDTTNNLQDKLHSNTEYFVKRMKDAGFDIKPTESAICAVMLYDAK
                     LSQDIAARLLDEGIYVTGFYYPVVPKDQARIRVQISAAHETEHLDKCIAAFTKVAKEF
                     NVIK"
     gene            complement(7840..8454)
                     /locus_tag="PKH58_03605"
     CDS             complement(7840..8454)
                     /locus_tag="PKH58_03605"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNX88143.1"
                     /translation="MKRSNYTAILFFITTIILAVLLFQAKRTERKSVYDSTTVLNKIV
                     YVQELSLVKYNYAGVISFKDYMKIMDIQIPLTEKFFLLKYNGYIKAGVDFKNIKIKVE
                     GKNIYVQMPKSRVLETVIDEKSIRVFDESMNAFNPLSINDYNKAITKEKVSMTRGAIE
                     QGILTEADNQAHLVITSLLKEMNFEKITIDELKTVELPEIEQQN"
     gene            9004..9714
                     /locus_tag="PKH58_03610"
     CDS             9004..9714
                     /locus_tag="PKH58_03610"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HNX88144.1"
                     /translation="MIFELKKFLVVVAVIITALPCISGLKGPSQNTKPDASIIPLRKN
                     SSVEMVYDSLHLDQKGLSRVAFDYAISGYKELKSKGILHNDSVLTIIDFDQPSYNKRM
                     YIIDIKNYKLLFNTLTAHGRNTGLETAKYFSNSPESFKSSLGLYITDQTYNGKNGYSL
                     KLKGLEKINNNAMNRAIVLHGAPYVSQRMINSRGYIGRSHGCPAVPQELNIPIIEKIK
                     GGSCFFIYNNSYHSDNTI"
     gene            9865..11130
                     /locus_tag="PKH58_03615"
     CDS             9865..11130
                     /locus_tag="PKH58_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNX88145.1"
                     /translation="MTPLAERMRPNTLDQYIGQKHLVGENAILRLMIDSGHISSFILW
                     GPPGVGKTTLARIIANQLERSFYTLSAVTSGVKDVRDVIEKAKSSRFFSQANPILFID
                     EIHRFSKSQQDSLLGAVENGTVTLIGATTENPSFEVITPLLSRCQVYVLKPLDDNDLL
                     ELANKAITTDPELNKRKFIIDEKEAMLRFSGGDARKLLNIIELIANSESDIEVHITNE
                     IVTKRLQQNPQAYDKDGEVHYDIISAFIKSIRGSDPDAAIYWLARMVAGGEDPKFIAR
                     RLVISAAEDIGLANPNALLLANACFDALQKIGWPEGRIILAQTTVYLASSPKSNSAYL
                     AIDNALELVERTGNLPVPLHLRNAPTKLMKNLDYGKGYKYAHDFENNFVNQQFLPDKI
                     KNERIWKVQENPAEKKMNEWLQFLWKGRY"
     gene            11170..12033
                     /locus_tag="PKH58_03620"
     CDS             11170..12033
                     /locus_tag="PKH58_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX88146.1"
                     /translation="MKKLTILLLSLLSVSGFAQQISNNPSIINVMTFNVRLDTEADGL
                     NNWKYRKEIAANTITFYQADIVGTQEVLHNQLTDLENLLPDYSSIGVGREDGMEKGEY
                     SAIFYNNKKLTETGSGYFWLSENPLSIGQKGWDAACERIATWAKLKDNQTGKIFFVLN
                     THFDHIGKIARRESAKLILNKVKLYSKKMPVIVMGDFNSEPESEVIQILTDTSNPLCL
                     TDSYRVSPIVQGPEWTFHEFGRLPLIERMRIDYIFVKNNVKVLSYKVIDEKVDNKYIS
                     DHNPVLVQVRF"
     gene            complement(12106..12843)
                     /locus_tag="PKH58_03625"
     CDS             complement(12106..12843)
                     /locus_tag="PKH58_03625"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNX88147.1"
                     /translation="MIYFIADAHLGSRLVKDPRKHEMQLVNWLDKIKHDAEAIYMLGD
                     MFDFWFEYKTVVPKGFVRFLGKMAELTDRGIEIHYFTGNHDIWTFGYLEQEIGLIVHR
                     SNETIEINGKKFFLAHGDGLFLEDKGFKLIRTVFHSKTCQQIFRMIPPAIGQNFGYTW
                     SKNNREKIMHLENKYQGEENEILVVFAKKYIKTHKVDYLLFGHRHVELDLQLENQSRI
                     IILGDFIGIYSYGVFNGEDLWLETYSE"
     gene            complement(12901..13215)
                     /locus_tag="PKH58_03630"
     CDS             complement(12901..13215)
                     /locus_tag="PKH58_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNX88148.1"
                     /translation="MNEFLEKEEKIVRMLKTVFDPEIPVNIYDLGLIYRIEPHDDGTV
                     DIDMTLTAPNCPAVDFIVEDVKMKVESVEGVTEAIIKIVYDPPWDKEMMTEEAKLELG
                     FL"
     gene            complement(13276..13686)
                     /locus_tag="PKH58_03635"
     CDS             complement(13276..13686)
                     /locus_tag="PKH58_03635"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNX88149.1"
                     /translation="MIDKYRYELEFKVRDYECDLQGIVNNAVYQNYLEHTRHEFLLKH
                     NVSFSDLHNQGIDAVVARVDMAYKYPLRSRDEFVCKLYVVKDGVKYVFHQAIYRIPDN
                     KLCVTAKVTTVVTINGRLASGYELLDGLTEVNNN"
     gene            complement(13701..14327)
                     /locus_tag="PKH58_03640"
     CDS             complement(13701..14327)
                     /locus_tag="PKH58_03640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNX88150.1"
                     /translation="MTIEDYILQNIDPEPGYLTELNRNTWTRILNPRMCSGHLQGRVL
                     NMLCKMIHPVNVLELGTFTGYSALCIAEALEETGILDTIESDDELEEFILSNFENTPY
                     RNIINLHIGDALKVIPTLGKIYDLVFIDADKREYSAYYNAVLPIIRPGGFIIADNTLW
                     SGKVVEKVPQNDHQTKGIMQFNQLIATDKRVEKVILPLRDGLTIIRKL"
     gene            complement(14327..15550)
                     /locus_tag="PKH58_03645"
     CDS             complement(14327..15550)
                     /locus_tag="PKH58_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX88151.1"
                     /translation="MSSYKTHTLSNGLRLVHLPNNSSVSYCGVIINVGSRDENDCDYG
                     MAHFIEHMLFKGTKKRNSRQIINRLEDVGGELNAYTSKEETVVYSGYLNEYSERAIEL
                     IADIVQNSLFPQYEIEKEIEIIQDEIQSYNDSPSELIFDDFEEMLFFSNPMAHNVLGK
                     GETITGFDTARAIKFYNTHYTPDEMVFFSLGSVDFDKIIRWCQKYFISTSNENNVQLR
                     QIPDIQPAETVVLKKNTHQVHCMIGSRAYDIYHPDRLVLYLLNNILGGPGMNSLLNLS
                     LREKHGLVYNVEAEYQAFTDTGWWAVYFGCDPENAAKCEKLIRNELAGLCNNTITVQK
                     LKRYKLQLMGQMAISSENKENLALSLGKSYLRFGRFDELEEVRTKINAITPEQLQRVA
                     CEIFNPDKLTILKYN"
     gene            complement(15648..17246)
                     /locus_tag="PKH58_03650"
     CDS             complement(15648..17246)
                     /locus_tag="PKH58_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88152.1"
                     /translation="MKKGMFLALFVSAVTIVSAANKMAIDYYGAGEYATAKTHFLAGN
                     VDAMDSYYLGQIYFKQNKMDSAKYYFEKGLQLDATNAYNTIGLATIKMANDPKGADGM
                     LKTVSKLKQYKKDAGIFTAIAEAYQLNNNDLQTKAYLAKAKKADKKSALPYILEADKF
                     IAQKNNGEAAVRYENAIYFDPNSKAALLKLADLYETTRTQLAFDYLRKAIEVDPQYAI
                     GLSTLGEMSYRKGFYPQAMESYEKYLALVNPTPKDYAQYATILYFNKQYTKTLEAIDK
                     APQDYIMERLKMYCLYELGNYAEAEVVGTKFMQNTPVKDIISKDYTSFARTLAKVKKY
                     NEAAGNYEAAFKLDTTKLEYLSEAAKAFERAKNYSKAIENYQRVIVANPAHSMADIYT
                     LGAAYYNGGLDTVSIKDKAMRKVYLLKADSTFKSMSTKFPDHYLGYLSSARTNSALDP
                     ETTQGLAKPYYEKALEVMLPSQTERKSEILECYRYLGYYHYVKNELAQSKVYWTKVLE
                     IEPTDSISLQVIKSIDSSLKKGKQ"
     gene            complement(17334..18272)
                     /locus_tag="PKH58_03655"
     CDS             complement(17334..18272)
                     /locus_tag="PKH58_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNX88153.1"
                     /translation="MRNLSLIIVAIFSIFLISSCVKAKKDKNENTMTSGYIKIAVDQT
                     MQKVIQNEIDVFEGLYPAKIEPIFTTETEAIELLKKDSVRLIVTARKLSEGEMNYFLD
                     KKFQPKNVRIAIDGIALIIHPQNPDSIISADNVKKILTGEYTQWKEIFPGSKLGKIQV
                     VFDNAKSSTVRYAIDSICRDKPISQNLSALELNEEVVDYVSKSPGALGFIGVNLISDD
                     ADTTAIDFTKKIQVMRVSREDLADRYNSVQPYQYYIYTHEYPFVRDIYFIVNDPRGEL
                     PKGFSRFVSSDKGQRIIKRSGLLPSTMPISTVHITD"
     gene            complement(18508..19335)
                     /locus_tag="PKH58_03660"
     CDS             complement(18508..19335)
                     /locus_tag="PKH58_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX88154.1"
                     /translation="MAKDVNLTSQEWIDLIFEGKNQDYGAYTLRKSSPKRHLLSFLGV
                     AIVAVLIFTSASLINMINQNRKKESMTEVAQMSNISMKKAVVPKENIEKAFKAPPPPE
                     LKSSLKFTAPIIKKDEEVREENEVKSQEEITESKVAISVADVKGTNEETGVDIATLQE
                     HKVIVAEEEPEKVFEVVEQPPSFPGGDAALMEYLNNNISYPTIAQENSIQGKVTCSFV
                     VGKDGSIQDVRVERSVDASLDKEAVRVIRSMPKWIPGRQGGNAVKVKYYLPVSFRLQ"
     gene            complement(19361..20008)
                     /locus_tag="PKH58_03665"
     CDS             complement(19361..20008)
                     /locus_tag="PKH58_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX88155.1"
                     /translation="MSAEIQEKDSGGKKKAGHMKKLSTRIDFTPMVDMIMLLLTFFML
                     ATSMSKPQTMEIGMPTKDKIDDKDKSEAKASEAVTLYLSKDHKVYYFMGIPDLVKPNF
                     LKQTDFSPEGLRKILLSKNIAVVTKVNELKEKKLKLQVSKEEYEKQLSKLKSSPGTPV
                     VVIKPLDNSTYNDMISALDEMLICNVGKYAITELEPNDKTILKNSNVQYEVAPQN"
     gene            complement(20069..20686)
                     /locus_tag="PKH58_03670"
     CDS             complement(20069..20686)
                     /locus_tag="PKH58_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX88156.1"
                     /translation="MPKIKIARKSTWIDMTPMSDVMILLLTFFMLTSTFLKPDPVKVS
                     TPGSVSEISIPEKNIFSVLISPDGRVFLDIDNPDDRMTILEQVAAEYQINLTDKEKKD
                     FSIGTSFGVPMEQFKEFMAIPGEKQKAALPNFGVPTDSTDNQFQAWVKAAREVKGKDM
                     VIAIKADQSTPYKKIKAVMGSLQDLRENRYNLITSLREVSTAKPK"
     gene            complement(20793..21605)
                     /locus_tag="PKH58_03675"
     CDS             complement(20793..21605)
                     /locus_tag="PKH58_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX88157.1"
                     /translation="MAKQTATKKKQSKGLSAALVILLCFILGVIIYLFVFGAPSNFID
                     NNPKNNPVQGNMLGTIYKGGFVVPILLTWLLSVIVLSVERWIAINRCRGKADLTKFVA
                     NVKEKLELEDIDAAKELCAKQKGSVAAVVSAGLDTYKLMSKSAPELSKEQKVATINKT
                     IEEATALEMPTMNQNLPVIATFTALGTLTGLFGTVLGMIRSFSALAGEGGTDSAALSV
                     GISEALVNTAFGILTGALAVISYNFFTAKIDNISYAIDEIGFSIVGVFQSKH"
     gene            22088..24844
                     /gene="leuS"
                     /locus_tag="PKH58_03680"
     CDS             22088..24844
                     /gene="leuS"
                     /locus_tag="PKH58_03680"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNX88158.1"
                     /translation="MEYKFREIEQKWQQYWVKNKTYKTGIEHSKPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGYNVLHPMGYDAYGLPAEQYAIQTGQHPAVTTQK
                     NIARYREQLDKIGFCYDWDREVRTCEPDYYKWTQWAFIQMFNHYYDTDLQKALPISEL
                     VNKFKCNGTSNVNASCTEELVFTAEEWKLYSEIEQQKVLLNYRIAYLADLSVNWCPQL
                     GTVLANDEVVEGLSIRGGYPVEQRVMRQWSLRVSAYAQRLLEGLDSLEWTDSLKETQK
                     NWIGRSEGAEMTFQLADKDFAFNIFTTRADTIFGVTFMVLAPESDLVRICTTDEYAEE
                     VNNYITATKKRTERERIADRRVTGVFTGSYAINPVAGTKIPIWVSDYVLAGYGTGAIM
                     AVPAHDSRDYAFAKNFNLPIIPLIEGADVSQESFDAKEGILTNSGFLNGLTVKEAIAK
                     TKEYITEKGIGYVKVNYRLRDAIFSRQRYWGEPFPVYYKEGMPYMLDESKLPLELPEV
                     DKFLPTETGEPPLGRAKNWNTGEGYPLELNTMPGFAGSSAYYLRYMDPKNDKALVSKE
                     ACEYWQNVDLYIGGTEHATGHLIYSRFWNKFLYDLGLITKDEPFKKLINQGMIQGRSN
                     FVYRIQGTSTFVSLNLKDQYDTTAIHVDVNIVSNDILDIEKFKSWNPEYKTAEFILED
                     GKYICGWAVEKMSKSMFNVVNPDDIVERYGADTLRLYEMFLGPLEQSKPWDTNGIDGV
                     HRFLKRLWGLMFRDEKFSVSDENATAEELKILHKTIKKIGGDIETFSYNTSVSAFMIC
                     VNELYSMQCNKKAILEPLCIILAPFAPHVAEEIYHLLGNSGTVCDAEWPHYNEEFLKE
                     NSVKYPVSFNGKVRFTLELPSDMNKNDVEKSALTCEQTQKYLEGKAPKKVIVVPGKII
                     NFVI"
BASE COUNT         7713 a         4836 c         4483 g         7898 t
ORIGIN      
        1 tcttgaaatg atccagctct tcgagcacaa ctatgggtat atgaacatca ttttcttcaa
       61 aattatgtaa acagttataa tcgtgcaata taacatttgt gtcaataaca aaattctttt
      121 tcgtatccat ggtttggaag atttaaatgt aataaaggga aaaattacgg tattatttta
      181 aatcaaactc tgaataaaag ttctcaccgt aaatatcgta agttattttc aaatatcaat
      241 caaaaaaaat aatgaaagta tcgaatattt ttgaccatta tcccatggtt ctgaaaagga
      301 aaaggtattg acccagccaa taaaaacaaa aaaccgataa tttgttatta tcggtttttt
      361 gcaatgtttt catttgtctg tttacagcat tattttttgt gtaaactttc ctgcacggac
      421 aatatagata cctttattca gatttaccag tgaaatgttt gttccattat atactactgt
      481 aaggacttta gcgcctaatg cactatatat atctatctgt acaccaattt ccagattagt
      541 tacattcagt gttttaccgg aaatcgtgaa cctgggaacg atctcattgt ccgaagtctt
      601 attactttgt gtattgtttg tttcggtagt gttcttatca aaaagcgtcg aattcttttg
      661 agattgaatt gccgttaatc cacaaagtaa aaaagttaat attagagtaa attttctcat
      721 aggcataatt tctttatact ttgtaatttc tactacaaag ataatgcaaa actatataat
      781 ccgtatcatt ttattttatt ttttttgatt ttaccacaac tgaagcccta tatgataact
      841 atttttttca tttcaatcat atttattaag caaacgcctt cattattaaa cacattaaaa
      901 actgtattac acgcatataa gtcataatta gtttattatt tttttgatta tctttgcatg
      961 ataaatttca attaataatg tagatgaaaa gaacagaacc ggaatatatt tttgaaacaa
     1021 gctgggaagt ttgtaatatg gtaggcggta tttacaccgt actctccaca cgtgctgaaa
     1081 cattatataa aaaattcaat gataaattga tctttatcgg acctgatatc tggaaagaca
     1141 ctgaatctcc ctatttcgaa ccctcagaca cattacactg tgaatggaaa aacaacttca
     1201 cagaaaaaac gggtctgaat gtacgttgcg gacgatggaa aatccccgga aatccgctga
     1261 cagtactggt cgattttact ccattgctaa atcgcaaaaa tgaaatattc ggtcatatat
     1321 gggataaatt cggagttgat tcgctgcatg gatatggcga ttatgatgaa tcgtccatgt
     1381 ttggatatgc caccggtatg attattgaaa gtttttaccg cttttacaat ctaagtgcta
     1441 aaaacaatgt aatagcacat ttcaatgaat ggatgacaac tttcggagca ttccatatta
     1501 aagaagttgt ccccgaaatt gcaacagtgt ttacaacaca tgccacttcc attggccgat
     1561 ccatagccgg caaccacaag cctttatatg actatttgag cgagtataac ggcgatcaga
     1621 tggcttttga actgaatatg gtagcaaaac attcaacgga aaaaacagct gctcatgttg
     1681 tggactgttt tactaccgtg agtaatatca cctctaaaga atgtaaacaa ttgttggaga
     1741 aagcacccca tatcattact cccaacggtt ttgacgattc cattgttcca acaggaaagg
     1801 acttcaatgt aaagagaaaa gcagcccgta gcaggctgaa aaaggttgct gaaaccctcc
     1861 ttggttacaa actgaaaaac aatgttttat ttatcaatac tgccggtcgc tacgaatata
     1921 agaacaaagg aattgacact tttatcgaat cgctgaaaag tctgaccaat catcctgaat
     1981 tgaacaggga agttgtggct ttcatcatgg ttcctgcgca tatttctggt ccaagagctg
     2041 atctggctgc aggtttgaat ggctcagagc tagcaataga ttactggaat aaacatacca
     2101 cgcacgaact gtatgattat caatacgacc atgtcatttc agcgctgcgc tggtttcact
     2161 ttaccaatct gcagaacgaa aaggttaaag tgattttcgt accatcctat cttaacggaa
     2221 atgatggcat ctttaacatg acatacctgg atttactcat tggtatggat gtaagcattt
     2281 tcccatcgta ttacgaaccc tgggggtata caccgctcga aagtgtggcg ttttccgtcc
     2341 ctacaattac ctccgataag tcgggatttg gtcaatgggt taaatccaat tatgatacaa
     2401 ggcacggtgt cgctgtagta catcgctccg actacaatca cacagagatg tgtgaggaaa
     2461 taggtgatat actggcaaaa ttcacaaatt acaattccga caaaacggat gatgtcagaa
     2521 aaaaggcatt agcgacctct aaacgggctt tatggagcgt atttattgat tattattatc
     2581 agacttacga aatagcatta aaaaaccgca atcacaggtt aaaacactga tatttaaaca
     2641 ttaataaagt ccgcatacgt ttgaaattta ttattatcaa ttttaactac acatcaccgc
     2701 aattttaaga ttatttttac tatttttgcc acgtattatg ttatagaaca tatttttgat
     2761 gtgttcttat gtcagattaa acccttttta caaaaataat aaacagtaaa ttccttatga
     2821 aacttaaagt caacagcaca aatgatatac cctggaagaa tattattata aaatcaaagc
     2881 ttcctgaaaa tctgaataaa ctggaagaaa tggcacaaaa catttggtgg tcatgggata
     2941 gtgaggccaa aaaattgttt cgtgaaattg atagaccagt ctggaaagaa gcagcttcaa
     3001 atcccgtcca attactgaat ctgcttaatt acgataaact ggatcagctt gctaacaatc
     3061 cggaatttat cgaaaaagta gacaatgttt acgctaaatt taaggcttac atgaatgagc
     3121 ctactaacac caaccgtcca tcaatagctt atttcagcat ggaatatgga ttgaacgaag
     3181 tgctgaaaat atattccgga ggtctcggcg ttcttgctgg tgactatctg aaagaagcca
     3241 gtgacagtaa tgtagatatg actgctgtcg gttttctgta tcgctacggt tatttcacac
     3301 agtcattatc ggttgatggt gaacaaattt ctgtctatga acctcagaat tttaatacat
     3361 tgccaataaa acaggtgatg gatgaaaata atcagccact ggaagtggaa gtaccctatc
     3421 ctggacgtac tgtgtatgct catgtttgga aagtttctgt aggacgtgtt tcactttatc
     3481 tacttgatac tgattatgat aaaaacagcg aatttgatcg ttccatcact caccagcttt
     3541 atggaggcga ctgggaaaac cgactgaagc aggaaatcat gcttggtatc ggtggtattc
     3601 ttgccctgaa gaaattggga attaagaaac aaatatatca ttgcaacgaa ggtcatgctg
     3661 cccttatcaa tgttcagcgt ctggttgatc tgattcacga cgaaaacctg acattcaatc
     3721 aggcacttga agtagtgcgt gcaagttcca tttacacggt tcacacacca gtacctgctg
     3781 gtcacgatag ttttgacgaa ggactctttg gtcgatatat gagtgaatat ccaggaaagt
     3841 tgcagatttc atggaatgag cttatggata tgggacgcga aaatcccggc gacaataacg
     3901 agaagttcaa catgagtgta ttcgcttgca acacaaacca ggaagtaaac ggagtaagct
     3961 ggctacacgg tgaggtatca aaaaagatgt ttgcccccat ctggaaagga tatttccccg
     4021 aagagtcgca tgtaagctat gtaactaacg gagtgcattt gccctcatgg acctcatccg
     4081 aatggaaagc tctttacgaa aaatacttca ctaaagcatt ctacgaagat cagtcaaatc
     4141 tgaaaatatg ggagaaaata tatcaggtac ccgatcagga aatatggaat ctccgtctgt
     4201 cgctcaaaaa taaacttgtt gaatacatcc ggatgcaatt tcaggaaaac tggatcaaaa
     4261 accagggtga tccttcacga atagtttcca tactagactc tataaatcca aatgctctga
     4321 tcattggttt cggacgtcgg tttgcaactt ataaacgcgc acatctgctg tttaccgacc
     4381 tcgaaagact ggaaaaaatc gttaatgatc ctattcatcc cgttcaattc ctttacacag
     4441 gtaaagcgca tcctgctgac ggtgccggga aaggactcat caaacgaatt gttgaaattt
     4501 cgcacatgcc gcagttcctc ggaaaaatta tcttccttga aaactatgat atgcgattgg
     4561 caaaacgtct gatttccggc gtggatatct ggctgaacac tcccacaaga cctcaggaag
     4621 catccggaac atcaggcgaa aaagctcaga tgaacggagt tctgaacttt tcggtactcg
     4681 acggctggtg gtatgaaggc tacaaaaaaa atgcaggctg ggcacttacc gaaaaacgta
     4741 ctttccagaa ccaggaattc caggataaac tggatgcgat aaccatttac aacatattgg
     4801 aaaacgaaat aataccttta tactttgcta aaaacacaaa gggatattca ccggaatgga
     4861 ttcaatatat caagaactca attgctcaaa ttacaccacg ttttacaaca aaacgcatga
     4921 tggatgatta tttcgaaagg ttctatatca agcaggcaaa acgttcaggt ttactgatgg
     4981 caaataatta taaggttgca aaagaaattg caaactggaa agaaacattg tcggctggat
     5041 ggaataatat agaagtaatc agcgtggaat cttccgaaga attccttaac aatccacagg
     5101 taggtgaaaa ttatgaaaca aaagttataa tcgacactaa gatgaatgag aaaagtgttg
     5161 gtgtagaaat ggtaaccatt tatacagata agaacaacaa tgatcacttc ttcaaagcca
     5221 ccgaattgaa ccttgtgaaa acggaaggtt ccaggatgta tttcagcctg acattcaagt
     5281 tacaacaagc tggtgtttac aaaacaagct accgcatatt ccctaaaaac gagttactgc
     5341 ctcatcgtca ggattttgca tacgtacgct ggttttgatg atttgaaata caattaaaaa
     5401 gacaatccct gtaaagctga actttacagg gattgtcttt ttaattgatt aactgatatt
     5461 attcccagtc taaaattact tttccacatt gtccgctttc catcacatca aaaccctttt
     5521 gaaattcgtt gattggaaaa cggtgagtta taaccggtgt cagatctaac ccggaaagga
     5581 gcatttgctg cattttgtac caggtttccc acatttcacg accatatatc cctttcaggg
     5641 tcagagcttt aaatattatt tcattccaat caacagtggt tgaattaggc aaaatgccga
     5701 gtaatgcgat cttcgatcca ttatacatat tgtcaatcat ctctctgaat gccgcaggtg
     5761 cccccgacat ttcaagtcca acgtcaaatc cacgcaactt aagttctttt ctggcgcctt
     5821 caacggtttc tccttttaat ccattcacag tacaggtagc tcccattttt tttgcaatgt
     5881 tcaatcggta ttcactcaga tcagtaacaa cgatattacg ggctccaccg aatttacaga
     5941 tagctgtagc taacgttcca atcaaaccgg cacctgtaat caggacatct tcgccgagaa
     6001 ccggaaatga caaagcagta tgtgttgcat ttccaaaggg atccattatc gaagcaattt
     6061 cgtctgagat acgttcatcc agatgaacca cattcgactc gggtaacgac agatattcgg
     6121 caaaagctcc atcgcggttt actccgactc cgattgtatt ttcacagaca tgaagttttc
     6181 ctctacgaca gttacgacaa tgaccacagg caatatgccc ttcaccggta acacggtctc
     6241 cgattttcac atttttcact ccggctcctt tatcgacaat cacacctaca tattcgtggc
     6301 cgatggtcat gggagtttta attgttttct gagaccagtc atcccattta tagatatgga
     6361 gatcggtacc acaaatagct gtttttttta ttttaatcag tacatcatta attccaattt
     6421 ccggaacggg aacattttcc atccagatac ctttttcggg cctgagtttt actaacgctt
     6481 tcatataata tatttggttt tcaaattaaa ttatcagaaa aagaaaaccc tgtttataat
     6541 tggtttattt gattacatta aattcctttg ccacttttgt gaatgcagca atacatttat
     6601 ccaggtgttc tgtttcatgc gctgcagaaa tctgaacacg aatacgcgcc tggtcttttg
     6661 gaacgacagg ataatagaaa ccggtaacat aaataccttc gtcgagcaaa cgggctgcaa
     6721 tgtcctgcga gagttttgca tcataaagca tcacagcaca aattgccgat tcggtaggtt
     6781 taatatcaaa accggcatct ttcattcgct tcacaaagta ttcggtattg gagtggagtt
     6841 tatcctgcag attatttgta gtatcgatta attcaaacat tttaagacct gctgcagcta
     6901 ccattggtgg aattgagttt gaaaagagat agggacgtga acgctgacga agtaattcaa
     6961 tgatttcttt cttgccggtt gtaaatcctc cgatagcacc tccaaatgct tttccgagtg
     7021 ttcctgttat gatttcaatt ttaccacgta aatcataacg ttctgtcaca ccacgtccgg
     7081 tgcgaccaac aacgccagcc gaatgcgact cgtcaatcat gaccattgca ttatattttt
     7141 ctgctaattc gtaaatttta tcgaggggag cgacatttcc atccatagaa aaaactccat
     7201 cggtaacaat taagcggaaa cgctgattct gggctttttt caactgatct tccaaatcgg
     7261 tcatatcagc attggcataa cggtaacgct gtgccttgca aagacgaacg ccgtcgatga
     7321 tagatgcatg attaagtgca tcggaaataa tggcatcatc ttcggtaaga agtggttcga
     7381 atactcctcc gttagcatcg aagcaggcag catataggat agtgtcttca gtgccgaaaa
     7441 aacgggctat tgaagcttcc agtttcttat gtaaatcctg agttccgcag ataaagcgca
     7501 cggaagagac tccgtagccg tgggtgtcaa tggcttgttt ggctgcatcc ttcagcaact
     7561 gattattggc taatcctaag tagttatttg cacagaagtt tagtacttct tttccatctg
     7621 caatacgaat aactgctccc tggggtgaaa ctataattct ttcgtttttg tacaatccag
     7681 cctcttttat tccagataat tctacctgta aatgtgattt ataatcctga tacatagcat
     7741 ttatttttat ttaatatatt ttttaaggca aatacaaagg tacagaataa atacataaat
     7801 agcgaaaaaa atcaattaaa tctgtggaat ttcgaaatgt cagttttgct gttcaatttc
     7861 gggaagttcg acagttttca gttcatcaat cgttattttc tcaaagttca tttctttaag
     7921 caaggaggta atgaccagat gtgcttgatt gtctgcttcg gtgagtattc cctgttcgat
     7981 tgcaccacgg gtcatgctta ccttttcttt ggttatagct ttgttatagt cgttgataga
     8041 gagcggattg aaagcattca tactttcgtc aaaaacccgt attgattttt cgtcgatcac
     8101 agtttcgagc accctggatt tgggcatctg tacataaata tttttacctt cgacttttat
     8161 ttttatattt ttaaaatcca ctccggcttt gatatagccg ttgtatttca ataaaaaaaa
     8221 cttttcggtg agtggaattt gtatatccat tatcttcata taatctttaa agctaattac
     8281 acctgcgtaa ttgtatttca caagagagag ttcctgtaca tacactattt tatttaagac
     8341 agtcgtacta tcatataccg attttctttc agttctcttc gcctgaaaaa gcaaaacagc
     8401 caatataatc gtagttataa aaaacagtat tgctgtataa ttactccttt tcataaattg
     8461 atatttttat tttttaaaaa aattaccaca aatacttatt tgcccgtaaa aatatataat
     8521 aaagctatta ataatcccac agaaccagct atggctaaaa atattttaat catgaggatt
     8581 ataaatgctc tgttatccat tccattctct ttcattttcg ttcaaattta attgttataa
     8641 aaaatagcac tttataacaa agataatatt atttttctac taatatttat tttggacaag
     8701 ggtgattata tttttatttg cagactgaat attggcagaa ttaatcaatt acagaattcg
     8761 ggtactcacg gattaaaccg ttattaaatg tttttattac acctttctat atttattgat
     8821 taaagaccca cgatgaaggt aaaattatca atattggtct gaaattatat taaataatcc
     8881 gaatttaaaa gttaatacga actgttaatc agcacattaa cttttattct ttgtattttc
     8941 gcataattat ttttgcaaaa gttcgtttat ggactaatta ctaattcatt tactgtttct
     9001 tttatgattt ttgaattgaa aaagtttctt gtagttgttg ctgttattat cactgctctt
     9061 ccttgtattt caggcttaaa aggtccttca caaaacacaa aaccagatgc tagtattatt
     9121 ccgctaagaa agaactcatc agtcgaaatg gtatatgaca gcttgcatct cgatcagaag
     9181 ggactatcca gagttgcttt cgattatgcc atcagcggat ataaagagtt gaaaagtaaa
     9241 ggaatactcc acaacgattc ggtactaaca attattgatt ttgatcagcc aagttataac
     9301 aaacgaatgt acatcattga cattaagaac tataaattac ttttcaacac tttgactgct
     9361 catggccgga ataccggtct ggagactgcc aaatacttca gcaattcacc tgaatccttt
     9421 aaaagcagtc tgggcttata cataaccgat cagacttata atggaaaaaa cggatactcg
     9481 ctgaaactaa aggggcttga gaagattaac aacaatgcca tgaacagagc cattgtactt
     9541 cacggggctc cgtacgtgag ccagcggatg attaacagca gaggatatat cggacgcagt
     9601 catggatgcc ctgcagtacc acaggaactc aatatcccaa ttattgagaa aattaaaggg
     9661 gggtcctgtt ttttcattta caataattca tatcattccg acaatacaat ttagaaagaa
     9721 ttaactcaca caaaagagcc ggataaaatt cggctctttt tataactggt ttatatgtac
     9781 tccggcaatg gctgaaaaat attttgtatt tttgtaaaac aataatcgta ttttcagttt
     9841 tatcatcatt aaagcatcat atcaatgact ccacttgcag aacggatgcg tccgaacaca
     9901 cttgaccaat atatcgggca aaaacacctt gtcggagaaa atgccattct gcggttgatg
     9961 attgactccg gacatatatc atcattcatt ctctggggac ctccgggggt aggtaaaacc
    10021 acgcttgccc ggattattgc caatcagctt gaaaggtctt tttatacatt gagcgcggta
    10081 acttcgggtg ttaaggacgt acgtgatgta attgaaaaag ctaaatcaag ccgtttcttc
    10141 tctcaagcca atcccatcct gtttattgat gaaatacacc gtttcagcaa atcgcaacag
    10201 gattctctgc ttggtgcagt ggaaaacggt acggttactc tcattggcgc taccaccgaa
    10261 aatccttctt tcgaggtgat tactcctcta ctttcacgct gtcaggttta tgtacttaag
    10321 ccactggatg ataatgacct gctcgaactg gcaaataaag ccatcacaac agatccggaa
    10381 cttaataaaa gaaagtttat aattgatgag aaagaagcaa tgctgcgctt ttcgggaggt
    10441 gatgcacgta aactgctgaa tattatcgaa ttaattgcca attcggagtc ggacattgag
    10501 gtgcatatta caaatgagat tgtaacaaaa cgactgcagc aaaacccaca agcttacgac
    10561 aaggacggtg aagtccacta cgatatcatt tctgctttta ttaaatcaat ccgtggcagc
    10621 gatccggacg cagccatcta ctggctggcg cgaatggtag ccggaggcga agaccctaaa
    10681 tttatcgcgc ggcgtctggt aatttcggct gccgaagata tcgggcttgc aaatcccaat
    10741 gctttattgc tcgccaatgc ctgttttgat gctttgcaaa aaataggctg gcccgaggga
    10801 cgaattatac tggcccaaac aaccgtttat ctggcgtctt cacccaaaag caattcagca
    10861 tacctggcta tcgataatgc gcttgaactt gtagaaagaa ccggcaacct gcccgttccc
    10921 cttcatttgc gcaatgctcc tacaaaactg atgaaaaatc tggattacgg aaaaggttac
    10981 aaatatgcac acgactttga aaacaatttt gtgaaccaac agttcttacc cgataaaata
    11041 aaaaatgaac gtatctggaa agtacaggaa aaccctgctg aaaagaaaat gaatgaatgg
    11101 cttcagtttt tgtggaaagg cagatattaa tctggcacaa agattgatat taagtaaaaa
    11161 aaaagaaata tgaaaaaact tactatcctg ctacttagct tgctttcagt tagcggcttt
    11221 gctcaacaaa taagcaacaa tccttcaata ataaatgtga tgactttcaa cgttcggctg
    11281 gataccgaag cggacggttt gaacaactgg aaatacagga aggaaattgc tgccaatacg
    11341 ataaccttct atcaggcaga tattgtggga actcaggaag ttctccacaa tcagcttact
    11401 gatctggaaa atttattgcc ggactattct tccatcggag tcggacgtga ggacggcatg
    11461 gaaaagggtg aatacagcgc aatattctac aacaacaaaa agctgacaga aacaggctcg
    11521 gggtacttct ggctgagcga aaatccattg tcaatcgggc aaaaaggctg ggatgcagcc
    11581 tgcgaacgga ttgctacatg ggcaaaactg aaagataatc aaaccggaaa gatatttttt
    11641 gtactgaata ctcattttga ccatattggg aaaattgccc gcagagaaag tgccaaactc
    11701 atactcaata aagtgaaact ctattccaaa aaaatgccgg taattgtcat gggtgatttc
    11761 aattcagaac cagaatcgga agtaatccaa attttaaccg acacatcaaa tcccttgtgc
    11821 cttaccgatt cgtaccgcgt ttcgcccatt gttcagggtc ctgaatggac attccacgaa
    11881 ttcggacgtc ttccccttat tgaaaggatg cgaatcgatt atatttttgt aaaaaacaat
    11941 gtgaaagttt tgagctataa agtgatcgat gaaaaagtgg ataataaata tatttcggat
    12001 cacaatccgg ttttagtaca agtccggttt taaaaaatca gcaaataaaa caaaaagaaa
    12061 caggcttaac tcttcattga attaagcctg tttatttcta taaaattatt ctgaataagt
    12121 ttcgagccat aaatcttcac cattaaatac gccatatgaa taaataccta taaaatctcc
    12181 caaaatgata atccggcttt gattttcaag ctgcaaatcg agttcaacat gccggtgtcc
    12241 gaatagcaga taatcaactt tgtgcgtttt aatgtatttt tttgcaaaca ccacaaggat
    12301 ttcattttcc tcgccctgat acttattttc gagatgcatg atcttctctc tgttgttttt
    12361 tgaccaggta tatccgaaat tctgaccgat agcaggcgga atcatacgga aaatctgctg
    12421 acaggttttg ctatgaaata cagttctgat aagcttaaat cctttgtctt caaggaaaag
    12481 tccgtcgccg tgtgccagaa aaaatttctt tccattaatt tcaatcgttt cgttgctgcg
    12541 atgaacaatt aatcctattt cctgctcgag ataaccgaaa gtccagatat cgtggtttcc
    12601 ggtaaaataa tgaatctcaa tgcctcggtc agtaagttct gccattttgc caagaaacct
    12661 aacaaaacct ttgggaacga cagttttgta ttcaaaccag aagtcaaaca tatcgcccag
    12721 catgtatata gcttctgcat catgcttaat cttgtcaagc cagttaacca gttgcatttc
    12781 atgtttgcgc ggatctttta ccaaccgtga cccaagatgt gcatcagcaa taaaataaat
    12841 catgaaataa atttaatagt ttttgatata aaataagtta tggatgtttc tgtctggatg
    12901 ttacaaaaaa ccaagttcaa gttttgcctc ttcggtcatc atttctttat cccagggcgg
    12961 atcatatact atttttatta tggcttcagt tactccctca acgctttcaa ccttcatctt
    13021 cacatcttcg actataaaat ctacagccgg acaattcgga gcagtcagcg tcatgtcaat
    13081 atcgactgtg ccgtcgtcat gaggttcaat gcgataaata agacctaaat cataaatatt
    13141 aacagggatt tcaggatcga aaactgtttt cagcatcctg actatctttt cttctttttc
    13201 taaaaattca ttcatattct gatatttgtt taacaaacaa attaattccg aaattgttgc
    13261 ctgaacaaca aacttttagt tgttatttac ttccgtcaac ccatcaagca gttcgtaacc
    13321 tgatgccagt cgaccattta ttgtaacaac tgtagtgact ttggcagtta cgcacagctt
    13381 attgtcgggt atgcgataaa tggcctgatg aaacacatat ttaactccgt cctttaccac
    13441 ataaagtttg caaacaaact catcacgcga acgcaacgga tatttgtatg ccatgtcaac
    13501 ccttgccact acagcatcaa tcccctgatt gtgcaaatca gagaaactca cattgtgttt
    13561 taataaaaac tcatgccgtg tatgttcgag ataattctga tagactgcat tgtttacaat
    13621 accctgcaga tcacattcat aatcccttac cttgaattca agttcatatc tgtacttatc
    13681 aatcattttc gttgttgttt ttataatttt cggattattg taagtccatc tcgaagtggt
    13741 aatatcacct tttccactct tttatcagtg gcaatcagtt ggttaaattg cataatgcct
    13801 ttcgtttgat gatcgttttg agggactttc tcaaccactt tgccggacca gagcgtatta
    13861 tcggcaataa taaatcctcc cggtcgtatt attggtaata cagcattgta ataagctgaa
    13921 tattccctct tgtcagcatc aataaaaacc aaatcgtaaa tttttcccag agttggtatc
    13981 actttcaaag catcaccaat atgcaggttg attatatttc tataaggagt attttcaaaa
    14041 ttactcaata taaactcctc cagctcatca tcactctcaa tggtgtcaag tattcctgtt
    14101 tcttctaaag cttcggcaat gcaaagcgcc gaataaccgg taaaagtacc aagttccagc
    14161 acattaacag gatgtatcat tttacaaagc atattcaaca ctcttccctg cagatgtcct
    14221 gagcacattc ttgggtttag tattcgtgtc caggtattcc ggtttaattc tgtcagataa
    14281 ccgggttcag gatcaatatt ttggagtatg taatcctcta ttgtcatcag ttatatttca
    14341 atattgtgag tttgtcagga ttgaaaattt cgcaggctac ccgctgtagc tgttccgggg
    14401 taatagcatt aatctttgtc ctaacttcct ccagttcatc gaagcgtcca aaacgcagat
    14461 agcttttacc cagtgataaa gccagatttt ctttattttc cgaagaaatt gccatctgtc
    14521 ccatcagttg caatttatat cttttcagtt tctgaacagt aattgtatta ttacataaac
    14581 cggcaagttc atttctgatt aatttttcac attttgcggc attttcagga tcgcaaccaa
    14641 aatacactgc ccaccatcca gtatcagtaa aagcctgata ttctgcttct acattatata
    14701 caagcccatg cttttcgcgt agtgataaat tcagtaaact gttcattcca ggccctccca
    14761 gaatattatt cagtagatac aaaacaagcc tgtctgggtg gtatatatca tatgcccgtg
    14821 aaccaatcat acaatgcacc tgatgggtat ttttctttaa cacaactgtt tcggctggtt
    14881 gtatatccgg aatttgtcgg agttgtacat tattttcatt tgatgttgaa ataaaatact
    14941 tctgacacca gcggataatt ttatcaaaat caactgaacc caacgaaaag aagaccatct
    15001 catcgggtgt ataatgagtg ttataaaatt ttattgctct ggctgtatca aatccggtta
    15061 tagtttcccc tttaccaagt acattatgag ccataggatt tgaaaagaac agcatttctt
    15121 caaaatcatc gaaaatcagc tctgagggac tgtcattata cgactgaatt tcatcctgta
    15181 ttatttctat ttccttttca atttcgtatt gtggaaatag cgaattctgt acgatatcgg
    15241 ctatcaattc gatagccctt tcggaatatt catttaagta tccggaatag actacagttt
    15301 cttcttttga ggtataggca tttaattcgc ctcccacatc ctctaagcgg ttaattatct
    15361 gacgggaatt acgttttttt gtccctttaa aaagcatatg ttctataaag tgagccattc
    15421 cgtaatcaca gtcattttca tcccgtgaac ccacattaat tatcactccg caataggata
    15481 cagatgaatt attgggtaga tgcacaagcc gtagcccatt cgacaaagta tgtgttttat
    15541 aactgctcaa tttacttcat tttaagtttg caaagttaac cattttttac taaatcaaaa
    15601 aagctgctta tacagcagct tttttgaagg tagtatttta atcgttttta ttgtttccct
    15661 tttttcagac ttgaatcaat acttttaata acctgtagcg atatggaatc agttggttct
    15721 atttccaata ctttggtcca gtatactttt gactgagcca gctcattttt aacataatga
    15781 taataaccca gataacggta acattccagt atctcagatt tacgttctgt ttgcgatggc
    15841 agcattactt caagagcctt ttcataatat ggttttgcca aaccctgagt agtttctgga
    15901 tctaaagcag aattggttcg ggcacttgaa agatatccca aataatgatc agggaatttt
    15961 gtactcattg atttgaaggt actgtcagct tttaataaat aaactttgcg cattgctttg
    16021 tcttttattg aaacagtatc cagtccgcca ttataataag cagcgcctaa agtataaata
    16081 tcggccatgg aatgtgcagg attagcaacg attacacgct ggtaattttc aattgctttc
    16141 gaataatttt ttgctctttc aaaggctttt gcagcttcac tcagatattc cagtttagtt
    16201 gtatccagtt tgaatgcagc ttcataattt cctgcggctt cattatattt ttttactttt
    16261 gccaatgttc tagcaaaact ggtataatct ttcgaaatta tatcttttac cggggtattc
    16321 tgcataaact tggttcctac aacttctgct tcagcatagt tgcctaattc atacaaacaa
    16381 tacattttaa gacgttccat gatgtaatcc tggggtgctt tatctatcgc ttcgagagtt
    16441 ttggtgtatt gtttattgaa ataaagtatt gttgcatatt gtgcataatc ttttggagtt
    16501 ggatttacca aagcgagata tttttcgtat gattccattg cttgtggata aaatcctttt
    16561 ctgtagctca tctcgcccag agtagataaa ccaatagcgt attgcggatc aacttcaatt
    16621 gctttgcgca ggtaatcgaa agcgagttga gttcttgtag tttcataaag atccgcgagt
    16681 ttcagtaatg cagcttttga attcggatca aaatagatag cattttcata tctcacggca
    16741 gcttctccgt tatttttctg tgcaataaat ttatcggctt ccagaatata aggcaatgcg
    16801 ctttttttat cagctttttt tgcttttgca aggtacgctt tagtttgtaa gtcattgtta
    16861 ttaagctgat atgcttctgc tattgcagtg aatatacctg catccttttt gtactgtttc
    16921 agtttcgata cagttttaag cataccatcg gctccttttg gatcgtttgc cattttaatg
    16981 gtggcaagac ctattgtatt gtaagcattt gtagcgtcta actgtaaacc tttctcaaaa
    17041 taatactttg ctgaatccat tttattttgt ttaaaataaa tctgccccag atagtaactg
    17101 tccatagcat caacgttacc agcgaggaag tgggtttttg cggttgcata ttctccggca
    17161 ccataataat caattgccat cttgttagct gcagaaacaa tcgttaccgc acttacaaac
    17221 aaggctaaaa acataccttt tttcattttt atcaatcttt taaaagttta tatttattct
    17281 ttatattatt cgttcaaaaa ttatgcctga aatctaaata ctatctgttt agttcaatcg
    17341 gttatgtgca cggtgctgat tggcatagtt gatggtagta aaccagaccg tttgattata
    17401 cgttgtcctt tgtcgctcga aacaaatctg gaaaagccct ttggaagttc gcctctcggg
    17461 tcattaacga taaaataaat gtcgcgaaca aatggatatt cgtgtgtgta aatataatac
    17521 tgataaggct gaacactatt atatcggtct gctaagtctt ctcttgaaac ccgcataacc
    17581 tgtatttttt ttgtaaaatc tattgcagtg gtatcagcat catcgctaat gaggtttact
    17641 ccgataaatc cgagagcacc cggtgatttt gaaacataat caacgacttc ttcgttcagc
    17701 tcaagggcac tcagattttg tgaaatcggt ttatcgcggc aaattgagtc tattgcatat
    17761 cttactgtgc ttgactttgc gttatcaaac acgacttgta tctttcctaa ttttgatccc
    17821 ggaaatatct ccttccactg tgtatattct ccggttaata tctttttcac gttatctgca
    17881 ctgataattg aatccggatt ttggggatga attatcagag ctattccatc aatagctatg
    17941 cgtacatttt ttggttgaaa tttcttatcg agaaaataat tcatttcacc ttcggaaagt
    18001 tttcgggctg ttacaatcag tcttacactg tcttttttca ataattcgat tgcttctgtt
    18061 tctgttgtga aaattggttc aattttagca ggatataatc cttcaaaaac atctatttca
    18121 ttttgtatta ctttctgcat agtctgatca acagctattt taatataacc gctggtcatg
    18181 gtattttcat ttttgtcttt cttggcttta acacaagaac ttattaagaa aatgctgaat
    18241 attgctacaa taattaaact aagatttctc attttcagat aattatatat gtttaaaaca
    18301 attctcacat gcgacatgtt ttgacaagtc agaaaattat aaaaaaataa cgatgacctt
    18361 tatgtatgtt tttcaacgat tgttcaccgg ctaagtctga acaaaaataa aaacaattat
    18421 atcaaactaa ttaacaaaaa caaaatttaa tctgaacctg tatttacaag ccggattaaa
    18481 ttttgtcttt actttatgat actgctatta ctgtaacctg aatgatacag gaagataata
    18541 cttaacctta acggcattac caccttgtct accaggtatc catttaggca tggatctaat
    18601 aacccttaca gcttctttgt cgagactggc atcaacactt cgttctactc tcacatcttg
    18661 aatagatccg tctttgccaa caacaaatga gcaggttact ttaccctgaa tactgttttc
    18721 ctgtgctatt gtaggataac tgatattgtt attcaggtat tccatcagag ctgcatcacc
    18781 cccggggaac gaaggtggtt gttccactac ttcgaatact ttttcgggtt cttcctcagc
    18841 tacgattact ttatgttcct gaagtgtggc aatatcaaca ccggtttctt cattggtacc
    18901 tttcacatct gctactgaaa tcgcaacttt ggattcggtt atttcttcct gagattttac
    18961 ctcattctct tctctaactt cttcgtcttt tttgattata ggagcagtaa atttcaaact
    19021 acttttcaac tcagggggag gaggagcttt aaatgctttt tcaatatttt ctttaggtac
    19081 aacagccttt ttcatactga tattcgacat ttgagcgact tctgtcattg attctttttt
    19141 tctgttctga ttaatcatat taatcagact agcagaggtg aagatcaata cagccacaat
    19201 agcaactcca agaaacgaaa gtaaatgtcg ttttggggaa ctcttacgga gggtataagc
    19261 tccgtagtct tgattttttc cctcaaaaat caaatcgatc cattcctgag aagttaaatt
    19321 aacatctttt gccatttttc atttattaaa tgataattaa ttaattctga ggagcaactt
    19381 catactgaac gttcgaattc ttcagtattg ttttatcatt aggctccagc tcggtaattg
    19441 catatttacc gacattacat ataagcattt cgtcaagtgc tgaaatcatg tcattgtatg
    19501 tagaattatc aagtggtttg ataactacaa ccggtgttcc gggactactt ttcagttttg
    19561 ataattgctt ttcatattct tccttagaaa cttgtaattt caactttttt tctttcagtt
    19621 cgttaacttt agtaactact gcaatgtttt ttgacaaaag aatttttctt aatccctcgg
    19681 gagaaaaatc cgtttgcttt aaaaagttgg gtttcacaag atcaggaata cccataaaat
    19741 aatatacctt atgatctttg gataaataaa gagtaacagc ttccgaagct ttcgcttcac
    19801 tcttgtcctt atcatctatc ttatcctttg taggcatacc tatttccatt gtctgcggtt
    19861 tactcatact tgtggcgagc ataaagaagg taagtaacaa catgatcata tccaccatgg
    19921 gggtgaaatc gatacgtgta ctcaatttct tcatgtgacc agcttttttc ttaccgccac
    19981 tatctttctc ttgaatttct gctgacatat tcttattttt ttacttcaaa acttaattca
    20041 ttattacgaa atgaagttta ttgatttttt actttggttt agctgtacta acttcacgca
    20101 atgaagtaat aagattgtac ctgttttctc gtaaatcctg aagagaaccc attactgctt
    20161 taattttctt ataaggagtt gactggtcgg ccttgatggc gataaccata tcttttcctt
    20221 ttacttcgcg tgctgctttc acccaagcct gaaattggtt gtcagtactg tctgtaggaa
    20281 ctccgaagtt aggtaatgct gctttttgtt tttctccggg tattgccatg aattctttaa
    20341 attgttccat gggcacacca aatgaagttc caatactaaa atctttcttt tctttatcag
    20401 tcagattgat ttgatattca gcagccactt gctcaagaat agtcattctg tcatcgggat
    20461 tatcgatatc cagaaacact cttccatcgg gtgaaattaa tacagagaaa atatttttct
    20521 caggaatact aatttccgag acagaaccag gtgtactaac ttttaccggg tcgggcttca
    20581 agaaagtgga tgtcagcatg aaaaacgtca agagcaaaat cataacatca ctcatcgggg
    20641 tcatgtcgat ccatgtactt tttcttgcta tttttatttt cggcattgtt ctttcgtttt
    20701 attaattaaa tgaatctaat tataatatcg gcaattaagt ataaattgaa cttatgctta
    20761 actactacgc ataggctatt ccgattcaaa tattaatgtt tcgactggaa aacacctaca
    20821 attgaaaatc cgatttcgtc gattgcgtaa gagatattat caattttagc agtaaagaag
    20881 ttataggaaa taacagccaa agcaccggtc aagataccga aagctgtatt aaccaaagct
    20941 tcagaaatac caacagaaag agcagctgag tctgttccac cttcaccagc caaagcggag
    21001 aatgaacgga tcataccgag tacagttccg aacaaaccgg taagagtacc aagtgctgta
    21061 aatgtagcaa ttacaggaag attctggttc atggtaggca tttccaaagc tgtagcttct
    21121 tcgatagttt tattgatggt agctactttt tgttctttac tgagttcggg tgcgcttttt
    21181 gacattaatt tataagtatc cagaccagca ctaacaactg cagctacaga gcctttttgt
    21241 tttgcacaaa gttctttagc agcgtcgatg tcttcaagtt cgagtttttc ctttacgtta
    21301 gcaacgaatt ttgtcaagtc agctttacca cgacatctgt tgattgctat ccaacgttca
    21361 acacttaata caattacaga aagtaaccat gttaaaagaa taggtactac aaatcctcct
    21421 ttataaatgg tacctaacat gttaccttga acaggattgt tttttgggtt gttgtcaata
    21481 aagttgctag gagcaccaaa tacaaataag taaataatta ctcctaaaat aaagcaaagg
    21541 agaatcacca atgctgctga taatcccttt gattgtttct ttttagttgc agtttgtttt
    21601 gccataatga tttaaaaatt gtgtgacctt agtattacta attccacggg tttaaaatta
    21661 aatttgattt attattaatt gaaatttttc tgaattatta aacttaacta ttttcttgta
    21721 aacgatataa ttaacagaat tattacatca tattaccgca tattatccga caaaaataat
    21781 atattttgcg gaataataac aacaatcacg aagatttttt gcacatttta atttcaataa
    21841 ttattgatct ggtctttcat aaaaagagaa aagggtgcaa atttagaata ttcatccata
    21901 tgattgagtt aatttggata tttaatcctc aatttagcat aaatattttc aagttgactt
    21961 attaaacgtc ataattgtaa tcacaaatta actaaatctg tagtatatca agctattttt
    22021 tgtaactttg caaactctta gaacaaacac aggacaaact tcgcatgtaa tttattatca
    22081 atttattatg gaatacaaat tcagagaaat agagcaaaaa tggcaacaat actgggttaa
    22141 aaacaaaaca tataaaacag gaatcgaaca ttcgaaacca aaattctatg tattagatat
    22201 gttcccctat ccttcgggag ccggattgca tgtcggacat ccgcttggct atattgcttc
    22261 tgacatttac agccgatata aaagattaaa gggttacaat gtgcttcatc cgatgggtta
    22321 cgatgcttac ggacttcctg cagagcaata cgctatacag accggtcaac atccggcagt
    22381 aaccacccag aaaaacattg cacgctaccg tgaacaactt gacaaaatcg gattttgcta
    22441 cgactgggac cgcgaagtca gaacttgtga accggactat tacaaatgga ctcaatgggc
    22501 atttatccaa atgttcaacc attattacga caccgattta caaaaggcat taccaatcag
    22561 tgaattagta aataaattta agtgtaacgg caccagcaat gtaaatgcct catgcaccga
    22621 agaactggtt tttacagctg aagaatggaa attgtattct gaaattgaac agcagaaagt
    22681 actgctgaat tatcgcattg cttatcttgc tgacctctcc gtaaactggt gcccgcagtt
    22741 aggaaccgtt cttgcaaacg atgaagtggt tgaaggtctt tcgattcgcg gaggctatcc
    22801 ggtagaacaa cgggtaatgc gtcagtggag tctgcgagta tccgcttacg cgcagcgtct
    22861 gctcgaaggg cttgacagcc ttgagtggac tgattcgctg aaagaaacgc agaaaaactg
    22921 gattggacgt tcggagggtg cagaaatgac ctttcaactg gcagataaag actttgcatt
    22981 taatattttc acaacacgtg cagataccat ttttggcgta acatttatgg ttctggctcc
    23041 cgaaagtgat ctggtcagaa tctgcactac cgatgaatat gccgaagaag tcaacaatta
    23101 tataacagct acaaaaaaaa gaaccgaacg cgaacgtata gccgaccgcc gtgtaacggg
    23161 agttttcaca ggttcttatg ccattaatcc cgttgctggg acaaaaatac cgatttgggt
    23221 aagtgattat gttcttgccg gatacggtac tggcgctatc atggctgttc ctgcacacga
    23281 tagtcgagac tatgcatttg ctaaaaattt caatctgcca ataattccgc tcatagaagg
    23341 agctgatgta agccaggaaa gctttgatgc caaggaaggc attctgacaa attcgggttt
    23401 cctgaacgga ctgactgtaa aggaggcaat cgcaaaaaca aaagaataca ttacagaaaa
    23461 aggaattggt tacgtaaagg ttaattaccg gttgcgtgat gctatcttta gtcgtcagcg
    23521 ctattggggc gaaccatttc cggtgtacta taaggaaggt atgccttaca tgctcgacga
    23581 aagcaaattg ccacttgaac ttcctgaagt tgacaaattt cttccgaccg aaacaggcga
    23641 accaccactt ggtcgtgcta aaaactggaa taccggcgag ggttatcctt tggaattgaa
    23701 taccatgccg ggatttgccg gatcatcagc ttattattta aggtatatgg atccgaaaaa
    23761 cgacaaagcg cttgtaagca aagaagcctg cgagtactgg caaaatgtgg atctatacat
    23821 cggaggaacc gagcatgcca ccggacacct aatctacagc cgtttctgga ataagttcct
    23881 gtacgatttg ggactaatta caaaagatga gcctttcaaa aaactgatta atcagggaat
    23941 gatccaggga cgaagtaact ttgtttaccg cattcaggga accagtacgt ttgtttcttt
    24001 gaatttgaaa gaccaatacg acacaaccgc tattcacgta gacgtcaata ttgtttcaaa
    24061 tgacatactg gatatcgaaa aatttaagtc atggaatccc gagtacaaaa ctgcagaatt
    24121 tatcctcgaa gacggcaaat acatttgcgg ctgggctgtg gaaaaaatgt ctaaatccat
    24181 gtttaacgtt gtaaatcctg atgatattgt tgaacgttac ggcgctgaca cgttgcggct
    24241 ttatgaaatg ttccttggac cgctcgaaca gtcgaaaccc tgggacacaa atggcattga
    24301 cggagttcat cgctttctga aaagactttg ggggttaatg ttcagagatg aaaaattttc
    24361 ggtcagtgac gagaatgcaa ccgcagagga actgaaaata cttcataaaa ccataaaaaa
    24421 aatcggtggt gacattgaaa cgttctcata caacacttca gtttcggcat ttatgatttg
    24481 tgtcaatgaa ctgtactcca tgcagtgcaa caaaaaagcg atacttgaac ctttatgtat
    24541 tattcttgct ccatttgctc cgcatgttgc cgaagaaatt tatcatctgc tgggtaattc
    24601 cggaaccgtt tgcgatgcag aatggccgca ttataatgaa gaatttctga aagaaaattc
    24661 ggtaaaatat ccggtttcat tcaacgggaa agtacgcttc acgctggaac ttccatcaga
    24721 catgaataag aatgatgtgg aaaaatcagc attaacctgc gaacaaacac aaaaatacct
    24781 ggaaggcaaa gcacccaaaa aagtaattgt tgtaccgggg aaaattatta attttgttat
    24841 ttagccagtt tatattcctt ttttcctgtg aataaaaaaa ttatttcagg acggggaaat
    24901 tgtttaatct atcaattacg gatacgaata
//
LOCUS       DAOPYB010000027        24166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2092_length_24166_cov_9.501887, whole genome shotgun sequence.
ACCESSION   DAOPYB010000027 DAOPYB010000000
VERSION     DAOPYB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 24166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24166
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2092_length_24166_cov_9.501887"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /gene="glyA"
                     /locus_tag="PKH58_03685"
     CDS             complement(<1..148)
                     /gene="glyA"
                     /locus_tag="PKH58_03685"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNX88159.1"
                     /translation="MVRDTKIFDIIEREKQRQMKGIELIASENFVSEQVMEAMGSVLT
                     NKYAE"
     gene            complement(231..770)
                     /locus_tag="PKH58_03690"
     CDS             complement(231..770)
                     /locus_tag="PKH58_03690"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNX88160.1"
                     /translation="MIYPLPAIMVSCGETEEEYNIVTVSWVGTICTNPPMCYVSIRPE
                     RHSHEIIKRTGEFVINLTNEDLAYATDWCGVKSGKKFNKFSEMKLTPVKGEMVNAPLI
                     KESPLCIECRVKEIISLGSHDMFIADVINVQADSKYINKKTDTFDLEKAKLIAYSHGH
                     YHKLGEEIGKFGWTVKKKK"
     gene            complement(918..1376)
                     /gene="pyrI"
                     /locus_tag="PKH58_03695"
     CDS             complement(918..1376)
                     /gene="pyrI"
                     /locus_tag="PKH58_03695"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNX88161.1"
                     /translation="MESKKLQVAALRNGTVIDHIPSDKLFQVFNILHLESCSNMITVG
                     NNLNSDKIGKKGIIKISDRFLEEEEASKIALIAPNAKINIIREFEVIEKRSLKLPDEV
                     RDIVQCQNPVCITNHQPVRTWFHMLDDEDSIQLRCHYCERDIKLEDVRIK"
     gene            complement(1381..2286)
                     /gene="pyrB"
                     /locus_tag="PKH58_03700"
     CDS             complement(1381..2286)
                     /gene="pyrB"
                     /locus_tag="PKH58_03700"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNX88162.1"
                     /translation="MKNRSLVSISDYSKEEILSILDSAADFEANPNRKTLDGKVIATL
                     FFEPSTRTRLSFETAVNRLGGRIIGFSDAATSSSVKGETLNDTIRMVSCYADAIVMRH
                     PLEGASRYAAEMSPVPIINAGDGANQHPSQTLLDLYSILKTQGTLEGLKICMVGDLKY
                     GRTVHSLLMAMSHFHPTFRFIAPDELKMPVEYKSFCRKNDIPYEETAELSDNFNDADI
                     LYITRVQKERFLDLIEFERVKNVYTLKNNMLDHTKPNLKILHPLPRVNEIDQDVDANP
                     KAYYFHQAQNGLYVRQAILCKVLNA"
     gene            complement(2532..3128)
                     /locus_tag="PKH58_03705"
     CDS             complement(2532..3128)
                     /locus_tag="PKH58_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNX88163.1"
                     /translation="MAESFRSITHLSQGIYKEKGSKFLSFAIPVKNVVEIKSILDIYR
                     KKYYDARHVCYAYMLGADRSEFRANDDGEPSGTAGRPILGQINSSELTDILVIVVRYF
                     GGILLGTSGLITAYKEAAADAIGQANIVEITVKNELNIRFEYPLLNDVMRIIKEYDVQ
                     IINQQFEEQCSIKISVPKKVTELISIKLEHIFGVNVEK"
     gene            complement(3128..4432)
                     /gene="murA"
                     /locus_tag="PKH58_03710"
     CDS             complement(3128..4432)
                     /gene="murA"
                     /locus_tag="PKH58_03710"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNX88164.1"
                     /translation="MSSFKIIGGQHLNGNITPQGAKNEALQVICATLLSDETITISNI
                     PAILDVLNLIQLLEYLGVKVDKIDENTYQFNASTINTEALQTEAFYQQSTSLRGSVML
                     VGPLVARFGKAIIPKPGGDKIGRRRLDTHFTGIQKLGAEFNFDPDKKLYRIEAKKLSG
                     CYMLLDEASVTGTANIVMTAVLAEGVTTIYNAACEPYIQQLCRMLNNMGANISGIGSN
                     LLTITGVKKLKGCSHTLLPDMIEVGSFIGMAAMTGSEITIKNVKYNELGIIPDSFRRL
                     GINLEQRGDDIFIPKQDSYTIESFIDGSIMTIADAPWPGLTPDLLSVFLVVATKAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLGEQLHLRAATMSSPDIRAGI
                     ALLIAAMCADGISTIHNIDQIDRGYQDIDKRLNALGAKITRI"
     gene            complement(4462..5058)
                     /locus_tag="PKH58_03715"
     CDS             complement(4462..5058)
                     /locus_tag="PKH58_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNX88165.1"
                     /translation="MEYPTLQGKLIMPEYGRNIQQMIAHALTIEDKEERTRCVNTIVN
                     IMGNLFPYLRDVNDFKHKLWDHVAIMSDFKLDIDYPYEILKQENLYSRPATIPYKNSR
                     IRYMHYGRNLEEMIERVATYPEGETKNEIIRMIANQMKKCFLSWNKEVVDDKKIFDDL
                     RELSHGKVDLSEENFKLVESRDVLQIGKKNKPKKNSGH"
     gene            complement(5138..6199)
                     /locus_tag="PKH58_03720"
     CDS             complement(5138..6199)
                     /locus_tag="PKH58_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX88166.1"
                     /translation="MKSTSKILILFSAAIFAISCGSGKSTVSTTKNKIEVQAVSVAFY
                     NLENLFDTINQSNIDEEFLPDGAMKWTGYKYKAKLKNMSYAISQLGLDITPHGASIIG
                     VSEIENRGVLEDLVKQPSIANRTYQIVHYDSPDRRGVDVGLLYNPRDFIVTSSKSIRL
                     HTPDTAFRTRDQLLVSGYLLNEKIHVIVNHWPSRTGGEVASEPKRMAAASLTKSIADS
                     LYKVDPNAKIIIMGDLNDDPFNKSTAEVLGAKRNIEDVAPNGLYNTLWRTLDSGVGSL
                     AYNSQWNLFDQIIISAPLLNKDKKKLSYWKSEVFNKSFLIQQEGSYKGTPMRTHAGGV
                     WLNGYSDHFPTLIYLVKEK"
     gene            6716..9469
                     /locus_tag="PKH58_03725"
     CDS             6716..9469
                     /locus_tag="PKH58_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX88167.1"
                     /translation="MKKVLLVISAVLLSTSAIFAQTQIKGVVKVADSETPLPGVTITL
                     LQQNISTKTIGSGEFLLTYLTAGVEEVSISKPGYLPQIKEVVLKENETLDLGTIFLKM
                     DLQEEMRQDAVLQLNESDFSEDEGRATQSISSSISRGDVYVSQTSFSFSPMRFRLRGY
                     EQEYESTYINGVHFNSLDRGGFNYSSLGGLNDAMRNKDVVVGLDANSFSYGNLGSNTN
                     IVNRASAFAAGTKASVAYSNRAYKLRGQVTYATGVLPSGWAFTVSGLFRWADEGIADG
                     TFYNSAGYFLAAEKIFNPRHSLSLVTYGAPTQRAQQSATTQEVYDLTGSIYYNSYWGY
                     QDGKKRNSRVVKSYDPTAILSHDFKISQNQRLRTGLAFHYSLYSNSAVGFYNAPDPRP
                     DYYRNLPSFLTDSANTGLRNEIAEQWKFENGKPVNPSVSQLNWDAMYQANYRNNAVNP
                     DGSARYSVERRHSNLMESVFNSTYTNQMNRNLKLTAGLEAKYAKDMNFKTMEDLLGGK
                     QWIDIDQFAERDYPSNPDIIQNDINNPNRAIRVGDVFGYKYDINIYHASAFLQNEWNW
                     SKLDLYYAAKLTYSQFSRYGYMKNGRAPENSYGQGKVWWFVDPSFKAGATYKLNGRNR
                     LYANVLAETRAPLPSGAYVSERIKDTRVPNLESRKILSYDLNYSFLFPSVRGRLTGFR
                     TYMLGTTENLGYYDDELRTFINHMLTKSDKIYQGIEAGVSVKLNRSFSVSTAGTFADY
                     RYTNNAIGIKSYENGVKPDFSEVVLTKDLKINSGPQLAANITLDYFHPKMWFADITLN
                     YFDNNYLDFAPNRFTESNMARYTTEEAKAALGSQEKLKGGFLLDASIGKLIYLKNKKS
                     INFNLSCSNILNNTSMITGGYQQARLPLVDNVLDMANLNKFPNKYYYAWGFNLFLNIG
                     YKF"
     gene            9489..10424
                     /locus_tag="PKH58_03730"
     CDS             9489..10424
                     /locus_tag="PKH58_03730"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HNX88168.1"
                     /translation="MKNKLIYSIMIAVTMLLGACNQTEFDAIPVGQLMGVKLDSTYTI
                     AQLKSEFLKSNSLFSADTIKSDKDVIIKGIVTSSDVEGNVYKYLVIQEEGNNAQAIKI
                     SIDAGSLSGIYPLGQRVAVKCNGMMIGNYAQSPQIGIYYYNTEKSRVEPGRMPKYLSD
                     KNIFAYGMPVPQVIKADTMTIAQIKAAGPALYNKLVCIKNAFFTGRGAYSGKPTAITD
                     AEMIFAPSTNGVGYPQSREIQDGTGSIFVSTSEYSKFANKPIPVSNLKGNITALVGFY
                     NDKDATLSTSKIYHQLTLRSISDLGKGFEAYHAAN"
     gene            10469..11932
                     /locus_tag="PKH58_03735"
     CDS             10469..11932
                     /locus_tag="PKH58_03735"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNX88169.1"
                     /translation="MKKYIKYGLFSLLSIAALSACSNVDEWEPPVSDATLIYEQAFAN
                     SLGDFTVQSVSGDTTWTNGGTYAIMSGYVNSVNLANEDWLISPVIDLTKTTAANLSFQ
                     HVVRYFTNLKDEATVWISTDYTDGLPATATWTQLTTQPFVDYQSWNFVPSGEISLTAY
                     AGKKVRIAFKYISTATKAGTWEIQNVVVKNTEAVVVTTNYGDGTEASPYSVTGGIYNQ
                     VSGKWVSGTIVGYVWSGTQTNFVFGSDTCTQATNLLLADSANNIYLSKTLPVQLPTGA
                     VRTALNLKDHKNLIGKKVKLYGDLASYFGVPGLKTVSYFELEDGTKGGNKPFDPSTAI
                     YYETFATGLGSFSAQNVSGSEVWVYNSTYKCAYMTGFVSNQNRANEDWLISPQIDLTG
                     KVSPALSFDHVVRYAGVPADEATVWVSENYTSGLPSTATWTKLPTAFTNAASWDFYNS
                     GDISLSAYANKKIRIAIKYVSTATKAGTWEMKNFIVK"
     gene            complement(12316..13536)
                     /locus_tag="PKH58_03740"
     CDS             complement(12316..13536)
                     /locus_tag="PKH58_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX88170.1"
                     /translation="MKKLILSLCLILGFSAAFAQKSNVAKAENYTLQEKPDFAGAREA
                     IKAAMQDEKTKADAKTYFTAGMIGFKENESFNTRLMVNDKSVDPMAKGKAIMEAYNYF
                     LIADSLDKLPDVKGKIKPRYGKKIKDNIKELYNGQHNIIAYGAQLFDNKNYKEAYNVF
                     NTYLTIPSLPVINKEVATDSTYNMIKYYAAIAATNNTDHTNAVKLYTDLKDDNYETKN
                     VYQLLSEEYRLMKDTVNYLNTLKEGVQKFSKDPWFLQSIINHYIYSDQIPEASKYLNE
                     AIALAPDVAQYYYVKGNVEERLGNNDTALEAFNKALELDPNMAGAYGGKGRVIFNKAV
                     QILNDASAIRDNKLYQKEKEKADVIFKEAMPFLKKAVEINPEDTDFKQALKQLYYRLD
                     MQAEYQAISKELGE"
     gene            complement(13617..>14864)
                     /locus_tag="PKH58_03745"
     CDS             complement(13617..>14864)
                     /locus_tag="PKH58_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNX88171.1"
                     /translation="KLRAEYEDLLKQIEHLKDILANIELRMEIIKNELIEIKAKYGDE
                     RKSEIVYASEEFNPEDFYSDDAMIITISHMGYIKRTALTEFRAQGRGGIGSKGSDSRD
                     EDFIENIYPASMHNTMLFFTQKGKCYWLKVYEIPEGTKSSKGRAIQNLLNIDADDKVN
                     AFIRVKGLDDEEYINSHYVIFCTKNGIIKKTSLKEYSRPRQNGVNAISIREDDDVIEV
                     RLTDGKNEIILANKNGRAIRFNEMKVRAMGRTATGVKGMTLDEDGADEVIGMICVNPE
                     GSENILVVSQQGYGKRSLLEDYRVTNRGGKGVKTINITEKTGKLVSIKSVTDENDLMI
                     INKSGIAIRTKVSDLRVMGRATQGVRIINLEKRNDEIASVCKVHSEDEEEVTSDKEVN
                     SESIDIESGINETETNETNETNQ"
     assembly_gap    14865..14964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14965..16002)
                     /locus_tag="PKH58_03750"
     CDS             complement(<14965..16002)
                     /locus_tag="PKH58_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNX88172.1"
                     /translation="MTDQEDRIIKVNIDDEMKSSYIDYSMSVIVSRALPDVRDGLKPV
                     HRRVLFGMNELGNNSDKPYKKSARIVGEVMGKFHPHGDSSIYGTLVRLAQPWAIRYTM
                     VDGQGNFGSVDGDGAAAMRYTEARLQKIAEEMLADIDKETVDFQPNFDDTLKEPTVLP
                     SRIPNLIVNGSSGIAVGMATNMATHNLSETVDACIAYIDNNEIEISELMKHIKAPDFP
                     TGGIIYGYSGVKDAFETGRGRIVIRSKTEIESNGSGHDKIIVTEIPYNVNKSELIKSI
                     ANLVTEKRIEGISNINDETNREGMRIVIDVKRDANASVILNKLFKMTALQSSFSVNNI
                     ALVNGRPRMLNI"
     gene            complement(16258..16716)
                     /locus_tag="PKH58_03755"
     CDS             complement(16258..16716)
                     /locus_tag="PKH58_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNX88173.1"
                     /translation="MIEKKTESCSNKNCCFELKDYGLLVLRLILGIFMLTHGWAKLSN
                     FSEMSQGFPAMLGMSPALGLSLIIFAEFFCSIALLFGFLTRLAVIPLIIGMSVAAFVA
                     HAGAPFNGRELPLLYLAMYVVLLLTGPGKIALDTLIYNRFCPHSKKGKEA"
     gene            complement(16815..17582)
                     /locus_tag="PKH58_03760"
     CDS             complement(16815..17582)
                     /locus_tag="PKH58_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HNX88174.1"
                     /translation="MTSFTYDKTFEGLLCCIFFAFEAKETPDILLGSHEVKPLFLDKS
                     FHIITETDKSARVWKGLEKKISASAQRMILTVWMSELPEADLLLFRYMTKAFNSSVSI
                     EVNFGDEDVLKCTEIYKKVNSSVHRVIQFVRFQKTKDGIYFAPIAPEYNTLAMTVEHF
                     RNRYADQRWIIYDTKRKFGLYYDLHTVTEITFTDNISPVNEDGKPDTGKYDDQELLFQ
                     KMWKQYFKTIAIKERINIRLQRQHMPIRYWKYLTEMQ"
     gene            complement(17643..18899)
                     /locus_tag="PKH58_03765"
     CDS             complement(17643..18899)
                     /locus_tag="PKH58_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HNX88175.1"
                     /translation="MSETTLEKLKILADAAKYDVSCASSGTTRDNKGKTLGTTSGWGI
                     CHSFTADGRCISLFKILLTNNCIYDCAYCANRRSNDIRRTTFTPKELAELTIEFYRRN
                     YIEGLFLSSGVIRTPDYTMEQMLSVVKELREVHKFNGYIHMKSIPGASQALVNKAGMY
                     VDRLSVNIEIPSEESLKIIAPEKDYKSVYGPMKYIKTGILENQEDRKKYRSAPKFVPA
                     GQSTQMIVGASPETDRQILSLSSRLYTIESMKRVYYSGYIPINNDSRLPMLKQPPLVR
                     ENRLYQADWLMRFYYFSSDEILDDKNPNLDLEIDPKLAWAIRNPQFFPIDVNTAPYEA
                     ILRIPGVGVLSAKKIIAARRFSRLGNEQLRKIGVVMKRAKYFIVSRELPVSTVQELQP
                     GYLRKLFTENKNTKIDTLQLAFDWGE"
     gene            complement(19008..19826)
                     /gene="ispE"
                     /locus_tag="PKH58_03770"
     CDS             complement(19008..19826)
                     /gene="ispE"
                     /locus_tag="PKH58_03770"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNX88176.1"
                     /translation="MLVYPNAKINLGLNVVEKRPDGYHNIETIFYPIGLHDVLSVETS
                     DTCSDYSFSVSGINIGGDAEDNLIVKSYRLLRSKYQFPPVDISLLKQIPFGAGLGGGS
                     SDAAFMLKALNEEFKLKITPKHLEQLASKLGSDCPFFIRNKPVYATGTGNIFAPADVS
                     LKGYILLLVKPDIHVSTAEAYAGITPQPSASPLTELIRKPIADWKHDIKNDFEKSVFE
                     KFPAIAAIKSELYQMGAIYASMSGSGSSVYGIFGEIPEYRDRFENCFVACGELE"
     gene            20015..21589
                     /gene="dnaB"
                     /locus_tag="PKH58_03775"
     CDS             20015..21589
                     /gene="dnaB"
                     /locus_tag="PKH58_03775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNX88177.1"
                     /translation="MAEKRNYNKKPASATPVPVNEFGKLPPQAPELEEDILGAIMLEK
                     DAYASIADILKPDFFYKTVHQKIYEAIVDLALHQNPIDMHTVTEMLRKKGTLDDVGGP
                     YYITLLTSRVSSAAHLIYHSQIIVQKYLARELIRMSSDIQTKAFDEKMDVDDLMQETE
                     AKLFEITQQNLKKDVVQINPVIEEARKRMKEAANRDGASGIPSGFIELDKITSGWQKS
                     DLIILAARPAMGKTAFVLSMTKNMAVDYNMPVALFSLEMSNVQLVNRLLMNVCSLEGE
                     KIKNGELSKEEWKKFDKDVTALYDAPIYVDDTPSLSIFELRSKARRLVKEHHIQCIII
                     DYLQLMNASGMSFGSREQEVSIISRSLKGLAKELDMPIIALSQLNRGLESRASNQNTD
                     GKRPQLSDLRESGAIEQDADMVCFIHRPEYYGMKVDKDGNSTEGIAELIIAKHRNGAV
                     GDVRLKFKNVYAKFINLGDEADPFAGQGYATFKSKLNSDEMTPDFYATPEMNGNPDYL
                     KPAPIGGSVNNNDVPF"
     gene            complement(21662..22957)
                     /locus_tag="PKH58_03780"
     CDS             complement(21662..22957)
                     /locus_tag="PKH58_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX88178.1"
                     /translation="MNLKFRLIIMNFLQFFVWGAWMMTLGHYGFVEKQWNGAEFGLVF
                     STMGFASLIMPTLFGIIADKWKANYVYALLHLLFGLTMCFLPLIDAPLPFFWVLFIAM
                     CFYMPTIGLNNSIGFTVLKNEGKDPTTYFPPIRVWGTVGFIAAMWITNVFTKEWGFGQ
                     SIKVSFIISAIFAFILSLFSFIFLPKSVRNEEKTVTVKKTLAQKLGLEAFILFKQKKM
                     ALFFIFSLLLGAALQLTNAYGDSFLQDANVFPKGELINNFSTIILSVSQISETLFILA
                     IPFFMKKFGIKKVMLFSMVAWVLRFGLFGIAQNSIPGFILIIASCIIYGMAFDFFNIS
                     GALFVEKNTDSKIQSSAQGVFMLMTNGVGAVIGNIVAGLVIAKWYEHPAIIEGKEILV
                     KDWQGIWLTFSAYALVVAVLFVVFFKYKHNPKETENFKH"
     gene            complement(22996..23598)
                     /locus_tag="PKH58_03785"
     CDS             complement(22996..23598)
                     /locus_tag="PKH58_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNX88179.1"
                     /translation="MTHEIKLHISGMVYNQSLGGTYSLVLAEDTGQKRRFSVLIGEAE
                     AQSIALKLNNAKSPRPLSHDLMNSIINMLEAKLTKVVIYDMVNDIFYSEIYLLHDNKT
                     IVVDARTSDAVALAVRTDAHIYINSDILDIVGTAIDTEQDAELETEISESISLDRLTN
                     EILVKLTDEKLKFFLEKALAEERYEIAAMIRDEIEYRKKH"
     gene            23772..>24166
                     /locus_tag="PKH58_03790"
     CDS             23772..>24166
                     /locus_tag="PKH58_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88180.1"
                     /translation="MKTTGILKQLIIRTWKLTADTTAAWDEIAAEKSTVKDVFLQYTI
                     PFILICTIISTLFGFLYADEKKLTIGILKGITIAVSLLAGNLLTYSVSIRTLSKYLPH
                     FAVKENVARLVVYSFSLMYFFRIVTAILPG"
BASE COUNT         6774 a         4839 c         4987 g         7466 t
ORIGIN      
        1 cttcggcata tttattggta agtacggagc ccatggcttc cattacctgt tcacttacaa
       61 aattttcgga agcgattagc tctattcctt tcatttgacg ctgtttttct cgctctataa
      121 tgtcaaaaat ttttgtgtct ctaaccatct tgttttaatt attattttat ttatttgatt
      181 ctaatttcct attatatttt accagtcttc atttgaagta ttttgaactt ttattttttc
      241 ttttttactg tccagccaaa tttgcctatt tcttcaccca gtttatgata atgaccatga
      301 gaatatgcaa tcagtttagc cttttcaagg tcaaaggtat cggttttttt gttaatatat
      361 tttgaatcag cctgtacatt tatcacatct gcaataaaca tatcatgcga tccgagagaa
      421 attatttcct tcaccctgca ttcgatacag aggggcgatt ctttgattaa cggggcgttt
      481 accatttcac ctttcaccgg cgtcagtttc atttcactga atttgttgaa ttttttcccg
      541 ctttttactc cgcaccaatc cgtagcataa gcaaggtctt catttgtcag gttaatgaca
      601 aattctccgg ttcgtttgat gatttcatgc gagtgtcttt ccggacgaat ggacacgtag
      661 cacatgggcg gatttgtgca gatagtacct acccagctta cagtcacaat attgtattcc
      721 tcttcggttt cgccacacga taccattata gccggaaggg gataaatcat cgtgccaggc
      781 ttccaggaaa tatggctgct ctgctcttca acaccctcaa gagagaacgt tgatgatttt
      841 gggtttataa ttgagttttg cttgtttttt tgcatttagt atacatgtaa ttcgtttatt
      901 tcgcgaatat tagtgattta cttaattctg acgtcttcca gtttaatgtc acgttcgcaa
      961 taatgacatc tcaactgtat actatcttca tcatccagca tgtgaaacca ggttcgaacc
     1021 ggttgatgat tggtgataca taccggattt tgacattgta caatatcacg tacttcatca
     1081 gggagtttaa gcgaacgttt ttcaatcact tcgaattcac ggataatatt gatttttgcg
     1141 ttcggagcga taagagcaat tttactggct tcttcttctt ccaaaaaacg atcagagatt
     1201 tttattatgc cttttttacc gattttatcg ctgttaagat tatttcctac cgtaatcata
     1261 ttactgcaac tttcaagatg caggatattg aaaacctgaa atagtttatc ggaaggtata
     1321 tgatcaatga cagttccgtt gcgaagagct gctacctgta atttttttga ttccatatgc
     1381 ttatgcgttt aatactttac aaaggatagc ttgtctgaca taaagtccgt tttgtgcctg
     1441 atggaaatag taagctttag gatttgcatc cacatcctgg tcaatttcat tgacacgggg
     1501 caggggatga agtattttta aatttggctt tgtgtggtcc agcatgttat tcttcagggt
     1561 gtaaacgttt tttacccgtt cgaattctat taaatcaagg aaacgttcct tttgtacacg
     1621 ggtgatgtac aaaatatcgg catcattgaa attgtcgctc agttctgcgg tttcttcgta
     1681 aggtatgtcg ttttttcggc agaatgattt gtattctacg ggcattttca gctcatcggg
     1741 agcaataaac ctgaatgtgg ggtggaagtg agacatggcc attagcaggg aatgaacggt
     1801 acgtccgtat ttcaggtcgc ctaccatgca tattttcagt ccttcaagcg tgccttgtgt
     1861 tttaagaatg gaatacagat caagcagagt ttgagaggga tgctgattcg ctccatcgcc
     1921 agcattgatg attggtaccg gagacatttc ggcagcgtaa cgggatgctc cttcgagtgg
     1981 atgtctcatc acaatggcat ctgcatagca actcaccatg cggatcgtgt cattgagtgt
     2041 ttcacctttt accgaactac tggtagctgc gtcagagaat ccgataattc gtcctccaag
     2101 ccggtttaca gccgtttcaa aacttagtcg ggtacgtgtt gatggttcaa agaacagggt
     2161 tgcaatcact ttcccatcca acgttttacg atttggattg gcttcgaaat cggctgccga
     2221 atccagtatc gacaggattt cttctttgct gtaatctgaa atggaaacaa gacttcggtt
     2281 tttcattctt ttttctgatg ataatctata ttattgtttt tttctcctga aacaaaaaac
     2341 caacataaat ctactgaaga tttacattgg tctgtatatg ttttggggcg aataatttat
     2401 gcaacttgct tgtgataagc tgctaaaaca gaaatcatat ttaattttta tctgtttcat
     2461 attcgaatgc aaagataatt ttttttttta ggaaaacaga gtgaatttat ttgaatgttt
     2521 tatgatgtat gtcatttttc tacatttact ccgaagatat gttcaagctt aattgagatt
     2581 aattctgtaa cttttttggg cacggaaatt tttatagaac attgttcttc aaattgctga
     2641 ttgattattt ggacgtcata ttccttgatg atacgcataa catcattaag caggggatat
     2701 tcaaaccgga tattcagttc gttttttacc gtaatttcaa cgatgtttgc ctgaccgatt
     2761 gcatcggcag cagcttcctt atacgctgta atcagtccgc tcgtaccaag taaaataccc
     2821 ccgaagtacc ggacaacgat aacaagtata tcggtgagtt ccgaggaatt tatttgccct
     2881 aaaatcggac gcccggcagt accggatggc tctccgtcat cattagcccg gaactcactt
     2941 ctatcagcac caagcatata agcgtagcaa acgtgccttg catcataata ttttttacga
     3001 taaatatcaa gaatggactt aatttcaaca acattcttta ccggtatggc aaaagaaagg
     3061 aatttacttc ctttctcttt gtaaattcct tgtgataaat gtgttattga cctgaaactt
     3121 tctgccatta tattctggta atctttgcac caagtgcatt cagtcgttta tcgatatcct
     3181 gataaccacg gtctatctgg tcgatattgt gaatggtgct gataccgtcg gcacacattg
     3241 cggcaataag taaagctata cctgcgcgta tatcgggtga actcatggtg gcggcacgca
     3301 gatgaagctg ttcaccaaga ccaatgactg tagcccggtg agggtcgcag agaataatct
     3361 gggcgcccat atctatcagt ttgtcaacaa aaaacagtcg gctctcgaac attttctgat
     3421 gtatcagtac acttcccttt gctttagtgg caacaaccag aaaaacactc aacaggtcgg
     3481 gtgtcagacc cggccaggga gcatctgcta tagtcataat cgaaccgtca ataaacgatt
     3541 cgatggtgta gctatcttgt ttgggtatga aaatatcatc ccccctttgt tctaagttga
     3601 tgcccagacg tcgaaaacta tcggggataa tgcccagttc gttgtatttt acatttttta
     3661 tggtgatttc agagccggtc attgcagcca tgccaatgaa actacccact tcaatcatat
     3721 caggcaacag tgtgtgactg catcctttca gtttcttaac accagtgatt gtcagcaggt
     3781 ttgatccgat tccggatata tttgccccca tattgtttag cattcggcaa agttgctgaa
     3841 tgtacggttc gcaggctgca ttgtagatag tggttactcc ttcagcaagc actgcagtca
     3901 tgacaatgtt ggcagtgcct gtaacagaag cttcatcaag caacatataa cagccactga
     3961 gtttcttagc ttcaatgcgg tagagttttt tatccggatc gaaattaaat tcggcaccca
     4021 gtttttgaat tccggtaaaa tgggtatcaa gtctgcgtct tccaatcttg tcacctcccg
     4081 gtttagggat tatagctttg ccaaatcggg ctaccaaggg tccgacaagc atgacagagc
     4141 ctctcagaga ggtactttgt tgatagaaag cttctgtctg aagagcttca gtgttaattg
     4201 ttgaagcatt gaactggtaa gtgttttcgt caattttatc taccttaaca cccagatatt
     4261 cgagtaactg aattaaattc agtacgtcaa gaatagccgg tatattgctg attgtgattg
     4321 tttcgtcaga aagcagggtg gcacaaatga cctgtaatgc ttcatttttt gctccctgcg
     4381 gggttatgtt gccgttcaga tgctgaccgc cgattatttt gaatgaggac attgctcgtc
     4441 ttgttctgta agttgtaaat gttagtgacc tgaattcttt ttgggcttgt tctttttacc
     4501 tatctgcagt acatctctgg actcaacaag cttgaaattt tcttccgaca agtccacctt
     4561 tccatgcgat aattcacgca ggtcgtcaaa aatcttttta tcatcaacca cttccttatt
     4621 ccaggataga aaacattttt tcatctgatt ggcaatcatc cggataattt cattctttgt
     4681 ttctccttcg ggataagtgg ccacacgttc aatcatttct tcgagattac gtccataatg
     4741 catataacga attctggaat ttttataggg tatagttgcc ggacgggaat acagattttc
     4801 ttgtttaagt atttcgtaag gatagtcaat atccagtttg aaatcagaca tgatagcaac
     4861 gtgatcccac agtttgtgtt tgaaatcatt cacatcgcgt agatatggaa acagatttcc
     4921 catgatattc acaattgtat ttacacatcg tgtacgctct tccttgtctt cgatagtgag
     4981 tgcatgtgca atcatctgct gtatatttcg accatattcg ggcattatca gtttaccctg
     5041 taaagttgga tattccatct tataatagtt ttgttatatt aattttcgga tatactagtg
     5101 tccaataatt tacttcaatc aggttgatga gccttaatta cttttctttt accagataga
     5161 taagggtggg gaagtggtcg ctgtaaccat tgagccacac gccacctgca tgtgtacgca
     5221 ttggagtacc tttgtaggaa ccttcctgct gaatcaaaaa gcttttgttg aaaacttcag
     5281 atttccagta actaagtttt tttttgtcct tattcaggag aggagctgaa ataataatct
     5341 ggtcaaaaag attccactga ctgttataag caagtgaacc tacaccgctg tcgagggttc
     5401 tccatagtgt gttgtataat ccatttggag ctacatcttc aatatttctt tttgcgccaa
     5461 gaacttcagc agtgctttta ttaaacggat catcattcag gtcgcccatg attatgattt
     5521 ttgcatttgg atcaacttta tacagcgagt ctgcaatgga tttggtcagg cttgccgctg
     5581 ccattcgttt aggttcagac gcaacttcac cacctgtacg cgaaggccag tgattgacga
     5641 taacatgaat tttctcattc agtaaataac cgcttaccaa caattggtcg cgggtgcgaa
     5701 aggctgtatc aggtgtatgc aggcgaattg atttactgct ggttacaata aaatcacggg
     5761 gattataaag taaacccaca tcgacacctc tacggtcagg gctgtcgtaa tggacgattt
     5821 ggtaagtacg attagcaatc gaaggttgtt tgacaaggtc ttccaaaacg cctctgtttt
     5881 caatttcgga aactccgatg atagatgcac catgcggggt aatgtccaaa ccaagttgtg
     5941 aaatggcata agacatgttt ttaagtttgg ctttatattt atagcccgtc cacttcatgg
     6001 ctccatcggg cagaaattct tcgtcaatat tactttgatt aatcgtatcg aaaagattct
     6061 cgagattata aaatgctaca ctaaccgcct gaacttcgat tttgtttttt gtggtactta
     6121 ctgttgattt tccagaacca cacgaaattg cgaaaattgc agctgaaaat aaaataagta
     6181 ttttgctggt tgatttcata tatttatttg taaaaattat tacaggtaaa attaaacaaa
     6241 agtccgaaaa aaacttgata taaaaaagta attgtataaa aacatcctgt aatttattta
     6301 actcaggggc ataaatttat ttctggaaag caaataatca tttgtctgaa agtaatattg
     6361 tacagggatt gagaataatt aagatatttt taataatgtt actatcgaat tgaaagttgg
     6421 gcgtttcttg tgaaaacaac tgataatcag aataaagtcg ggtatggtga tgactgcgag
     6481 catatgtgaa catcattttt atacagaaat gaaagtttgg ataagtatta atcaaaaaaa
     6541 agattgtatc ttgcacaact ttttctgttt taaaaaatgg taattgatat ataaaaatgg
     6601 agttaatcat ttagacgtta aacgagcatt taatcggact ttacctgtgg acatagaatg
     6661 ttcagggttt gataaaaatt ggaatcttca aaattaataa caaaaaaata tacaaatgaa
     6721 aaaagtactg ttggtcattt cagctgttct tttatctaca tctgcaattt ttgcccaaac
     6781 gcagattaaa ggtgtggtaa aagtagctga ctctgaaaca ccgctgccag gtgttacaat
     6841 cacccttttg cagcaaaata tttcaacaaa aacaatcggg tcgggggaat ttcttctcac
     6901 atacctgaca gccggagtag aggaagttag tatttcaaaa ccgggatatc tgccgcagat
     6961 taaagaggtt gttttaaagg aaaacgaaac actcgatctc ggaacaattt ttctgaaaat
     7021 ggatttacag gaagaaatgc gtcaggatgc tgttttacag ctgaacgaat ctgatttcag
     7081 tgaagacgaa ggacgcgcta ctcaaagcat ttcgtccagc atatcgcgcg gagatgttta
     7141 tgtttcgcaa acttccttct ctttcagtcc catgcgattc cgcttgcgtg gttatgaaca
     7201 ggaatatgag tcgacttata ttaatggtgt tcactttaac tcgctggatc gtggcggttt
     7261 caattattcc agcctcggag ggctgaatga tgccatgcgc aataaggatg tagttgtcgg
     7321 actggatgcc aattctttca gttacggaaa tttaggatca aatacgaata ttgttaacag
     7381 agccagtgca tttgctgccg gaacaaaagc aagcgtcgct tacagtaacc gggcttataa
     7441 actcagaggt caggtaacat atgccaccgg tgtgctccct agtggttggg ctttcacggt
     7501 ttctggcctt ttccgttggg ctgatgaagg tatcgccgat ggaacatttt ataattctgc
     7561 cggatatttc ctcgctgctg aaaagatatt taatccacgg cacagtctgt cgctggttac
     7621 ttacggtgcg cccacacagc gtgctcagca gagtgcaact acgcaggaag tgtatgatct
     7681 gacaggttcg atttattaca attcgtactg gggttatcag gatgggaaaa agcgtaactc
     7741 acgcgttgtg aaaagctacg atcctaccgc tattttgtcg catgatttca aaattagtca
     7801 gaatcagcgt cttcgtacag ggcttgcttt ccattacagc ctttacagca actcggctgt
     7861 cggattttac aatgctccgg atccacgtcc cgattattac cgcaatttac caagtttcct
     7921 caccgacagc gctaacacag gtttaagaaa tgaaatagca gaacagtgga agtttgaaaa
     7981 tgggaaaccg gttaatcctt cagtttcgca attgaactgg gatgccatgt atcaggccaa
     8041 ttaccgcaat aatgctgtaa atcccgatgg tagcgccaga tattcggtgg aacgccggca
     8101 cagcaatctg atggaatcgg tattcaattc cacttatacc aatcagatga atagaaatct
     8161 gaaacttaca gccggacttg aagcaaaata tgcaaaagat atgaacttca aaaccatgga
     8221 agacctgctt ggaggaaaac aatggataga tatcgaccag tttgcagaac gtgattatcc
     8281 atccaatcct gatattattc aaaacgatat caacaatcct aaccgggcaa tcagagttgg
     8341 ggatgtattt ggatacaaat atgatattaa tatttatcac gccagtgctt ttcttcagaa
     8401 tgaatggaac tggtcaaaac tggatcttta ctatgccgca aaactcactt acagtcagtt
     8461 cagccggtat ggctatatga aaaacggacg cgctcctgaa aattcatacg gtcagggtaa
     8521 ggtatggtgg tttgttgatc cttcgttcaa agcgggagca acctataaac tgaacggaag
     8581 aaacaggtta tatgccaatg tactggctga aacccgcgct ccacttccca gcggagctta
     8641 tgtgtcggag aggatcaaag acacccgggt tcccaatctc gaaagccgca agattttatc
     8701 gtatgatctg aattattcgt tcctgtttcc ttctgtacgg ggccgtctca caggtttccg
     8761 tacctacatg cttggaacta ccgaaaacct cggatattat gatgacgaac tccgtacgtt
     8821 tatcaaccac atgctcacca aatccgataa aatctatcag ggtattgaag caggggtatc
     8881 agttaaactt aacagaagtt tctcggtttc cacagcagga acatttgctg attatcgtta
     8941 taccaacaac gccattggta taaaaagtta tgaaaatggc gttaaacccg atttctcaga
     9001 agtggtacta accaaagatt tgaaaataaa ttcaggtccg cagcttgcag ccaatatcac
     9061 actggattat ttccacccga aaatgtggtt tgccgatata acactgaatt atttcgacaa
     9121 taattatctg gactttgctc caaatcgttt cactgaaagt aatatggcca gatacaccac
     9181 agaggaagca aaagcagcac ttggatcaca ggaaaaactg aaaggtggtt tcctgctgga
     9241 cgcttcgata ggaaaactaa tctatctgaa aaacaaaaaa tcgatcaact tcaatctgag
     9301 ttgcagcaat attcttaaca atacctctat gattaccgga ggttatcagc aggcacgttt
     9361 gccactggtt gataatgtgc ttgatatggc taatctgaat aaattcccga acaagtatta
     9421 ttatgcatgg ggattcaatc tgttcctgaa tatagggtat aaattttaat cgataagtga
     9481 agaagaaaat gaaaaataaa ctgatataca gtataatgat tgctgtgacc atgttacttg
     9541 gcgcttgcaa ccagaccgaa tttgatgcaa tacctgtcgg gcagctgatg ggtgtgaaac
     9601 tggactcgac ctacaccatc gcgcagctga aaagtgagtt tctgaaatcc aactcccttt
     9661 tctctgccga tactatcaaa tcggataagg acgttattat taaaggtatc gtaacctcat
     9721 cggatgtgga aggaaatgtt tataaatacc tggtcattca ggaagaagga aacaatgcac
     9781 aagctatcaa aatatccata gatgcaggaa gtctctcggg aatttatccg ctgggacaac
     9841 gcgtagctgt gaagtgcaac ggaatgatga taggcaatta cgcccagtca cctcagattg
     9901 gaatttatta ttataatacc gagaagagca gggtagaacc cggacgtatg ccaaagtatc
     9961 tgtccgataa aaatatcttc gcttatggta tgcccgttcc gcaggttata aaagccgaca
    10021 ccatgactat cgcgcagatt aaggctgccg gacctgcatt gtataataaa ctggtatgca
    10081 tcaaaaacgc ttttttcacc ggaagaggcg cttacagtgg caagccaaca gccattacag
    10141 atgccgaaat gatttttgcc ccgtcaacca atggcgtagg ttatccgcaa tcacgcgaaa
    10201 ttcaggatgg aacaggttct atcttcgttt caaccagcga atattcgaaa tttgccaaca
    10261 aacccattcc ggtttcaaac cttaaaggga atataaccgc tttagtggga ttttataatg
    10321 acaaggacgc taccttatct acatctaaaa tatatcatca gttgacttta cgatctatca
    10381 gcgatttggg taaaggattt gaagcttatc atgcagcaaa ttaaaaagta accgaacgca
    10441 tattaattaa aacaaaacat ttacagagat gaagaaatat ataaaatatg gtctattcag
    10501 tttattatcc atagcagcgc tatcggcctg ctccaatgta gatgaatggg agccacccgt
    10561 atctgatgcg acactgattt acgaacaggc atttgccaat tcgctgggcg attttacagt
    10621 gcaatcggtt agcggcgata caacctggac aaacggagga acttatgcca tcatgtcggg
    10681 ctatgtgaat tcggtaaacc ttgcaaacga ggactggctc atttcacctg taatcgacct
    10741 gaccaaaacg actgctgcca acttgtcgtt tcagcatgtg gttcgttatt ttacaaatct
    10801 gaaagatgaa gccactgtct ggatttcgac cgactataca gatggactgc cagctaccgc
    10861 aacttggaca caacttacca ctcagccttt tgtggattac caaagctgga attttgtacc
    10921 ctccggtgaa attagtctga ctgcctatgc cggtaaaaaa gtaagaattg cctttaaata
    10981 catttcaaca gcgaccaaag ccggaacctg ggaaatacag aacgttgtag tgaaaaatac
    11041 cgaagctgtc gttgtgacta caaattatgg tgacggaacg gaagcatccc cttattcagt
    11101 tacaggaggt atttataacc aggtatcagg taaatgggta agcggaacga ttgtgggata
    11161 tgtatggtca ggtacacaga caaactttgt atttggttct gatacctgta cacaagcaac
    11221 caacctgttg ctggctgatt cagccaataa tatttatctt tctaaaaccc tgccagtcca
    11281 acttccaacc ggcgctgtac gtaccgcgct caacctgaaa gaccacaaga atttgattgg
    11341 taagaaagtg aaactttatg gcgatctggc atcctacttt ggcgttccgg gtttgaaaac
    11401 agtgagttat tttgaactcg aagacggaac aaaaggagga aataaaccat tcgatccttc
    11461 gacagcaatt tactatgaaa catttgctac gggattagga tctttttctg cgcaaaatgt
    11521 atcgggatca gaggtctggg tatataattc gacttacaaa tgcgcttata tgaccggatt
    11581 tgtaagcaat caaaacagag caaacgaaga ctggcttatt tcgcctcaga ttgatcttac
    11641 aggcaaagtt tcaccggcgt taagtttcga tcatgtggtg agatatgcgg gtgtgcctgc
    11701 tgatgaggct actgtttggg tttcggagaa ttatacttcg ggattacctt cgacagctac
    11761 gtggacaaaa ttgcctacag catttacaaa tgcagcctca tgggatttct acaacagcgg
    11821 agatatttca ttgagcgctt atgccaacaa aaaaatccgt attgcgataa aatatgtttc
    11881 aactgctact aaagccggca catgggaaat gaagaatttt attgtaaaat aaaaaaatcg
    11941 aaatacaatc tcgttaatca ggctgtcagg tgaaacccga cagcctgatt tgttttatag
    12001 attatggata aatcatccgg aatacgcttc tttctgcatt ccgctttacc ggagaaagcg
    12061 tataggccgt gcgcaataac tgtcgctatg ataaagattg ttgcaaagaa taataaatca
    12121 ataaacaata ttgcaaaatt gaagtaatag ttacaaattt atttcgggta cttcccaact
    12181 atttccgtgt tttatgtggt atttctgcat accttccccc gttttcgcgt gaccttcgtt
    12241 gatttacgag gattattatc acaaaaaaag agtgctttgt ggcactcttt tttataatta
    12301 tatgatcagc ttattttatt cgccaagttc ttttgaaata gcctgatatt cagcctgcat
    12361 atccagacga taatagagtt gtttcaaagc ttgtttgaag tcggtatctt cgggattgat
    12421 ttctacggct ttcttcagga atggcattgc ttccttgaaa atgacatcag ctttttcttt
    12481 ttctttctga tatagtttgt tgtcacggat ggcgcttgca tcattcagaa tctgtactgc
    12541 tttgttgaaa atcacgcgtc ctttaccgcc ataagctcct gccatattcg gatcgagttc
    12601 aagtgcttta ttgaaagctt ccagcgctgt atcgttgttg cccagtcttt cttccacatt
    12661 accttttaca taataatact gagccacgtc gggagctaaa gcaattgctt cattcaggta
    12721 tttcgatgct tcaggtatct gatccgaata gatgtaatga ttgatgatgc tctgaaggaa
    12781 ccagggatct tttgagaatt tttgtacacc ttctttcagc gtattcagat aatttaccgt
    12841 atctttcatc aaacgatatt cttcggataa cagctgataa acattctttg tttcgtaatt
    12901 gtcatccttc aggtcagtgt acagtttgac agcgtttgta tgatctgtat tattggtggc
    12961 tgcaatagcg gcgtaatatt taatcatgtt gtaggtgctg tctgttgcta cttctttatt
    13021 tattacaggc aaagaaggaa ttgtcagata ggtgttgaac acattgtagg cttctttata
    13081 gttcttgtta tcgaataatt gagcgccata agcgatgatg ttgtgttgcc cgttgtaaag
    13141 ttctttaatg ttgtccttga ttttctttcc ataacggggt ttgattttac ctttcacatc
    13201 gggaagcttg tccagactgt cagcaatcag aaagtaattg taagcttcca tgattgcttt
    13261 tcctttagcc atcggatcaa cggatttatc attcaccatc agtcttgtgt tgaaagattc
    13321 attttcctta aatccgatca tacctgctgt gaagtaggtt tttgcatctg ctttggtctt
    13381 ttcgtcctgc atagcagctt taatagcttc gcgggctcct gcaaaatcag gcttttcctg
    13441 caatgtgtag ttctcggctt tggctacatt tgatttttgt gcaaatgcgg ccgaaaaacc
    13501 caaaattaaa cataatgata gaattaattt tttcatgttc gtaaaattgt ttttttgtta
    13561 agtttaattc atgccttttg aagaaaaatc aaacaagcat tactgtgttg attgaattat
    13621 tggtttgttt cgtttgtttc gtttgtttca gtttcgttaa ttccggattc gatatcaatt
    13681 gattctgaat ttacttcctt atcgcttgta acttcttcct cgtcttcgga gtgaaccttg
    13741 catacagaag caatttcatc atttcgtttt tcaagattta ttatcctgac accctgtgtg
    13801 gctctgccca taacccgcaa gtcggaaact ttggtacgga tggcaatacc tgatttgttg
    13861 ataatcatca ggtcattttc gtcggtcacg ctcttaattg ataccagttt acccgttttt
    13921 tcagtgatgt tgatggtttt tacacctttg ccaccacgat tggtaacccg gtaatcttcg
    13981 agcagcgaac gttttccgta accttgctgc gatacaacga ggatgttttc actaccttcg
    14041 ggatttacac aaatcatgcc aattacttca tccgctccgt cttcgtcgag agtcattccc
    14101 tttacaccgg ttgcagtacg tcccatggct ctcactttca tttcgttgaa gcggatggca
    14161 cgtccgttct tgttcgcaag gatgatttcg tttttaccat ctgtcaggcg aacttctatc
    14221 acatcgtcat cttcccggat cgaaattgca ttgacgccat tctgacgtgg gcgtgaatat
    14281 tctttcaggg atgttttctt aattatacca tttttcgtgc agaaaatcac atagtgcgaa
    14341 ttgatgtatt cttcgtcgtc caaccctttg acacggataa aggcattcac cttatcgtcg
    14401 gcatcgatgt tgagcaggtt ctgaatagct ctgccttttg aacttttagt tccttccggg
    14461 atctcgtaaa ctttcagcca gtagcatttt cctttttgag tgaagaacag catggtattg
    14521 tgcatcgaag ccggataaat gttttcaatg aaatcttcat cgcgcgagtc actgcccttt
    14581 gaaccaatgc caccacgacc ctgagccctg aattcggtca gtgcagtccg cttgatgtaa
    14641 cccatgtgcg aaatggtaat gatcattgca tcgtcagaat agaaatcttc gggattgaat
    14701 tcttccgaag catatacaat ttcgctttta cgttcgtcgc cgtactttgc tttaatttcg
    14761 attaattcgt tttttataat ctccattctg agttctatat tagccagaat gtctttcaaa
    14821 tgctcaatct gcttcaataa atcttcatat tcagctctca gtttnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnngatgtt gagcattcgc ggacgaccat tcaccagcgc
    15001 gatattgttt acgctgaatg atgattgaag cgcggtcatt ttaaacagtt tattcaggat
    15061 gacacttgcg ttggcatctc ttttaacatc aataacaata cgcatgcctt cgcggttggt
    15121 ttcgtcattg atatttgata tgccttcaat tcttttttcg gttaccaggt tagcgattga
    15181 cttgatcagt tccgatttgt tgacattgta ggggatttcg gttacgataa ttttatcgtg
    15241 tccgctgcca tttgattcaa tctcggtttt cgaacggatt acaattctgc ctcgtccggt
    15301 ttcgaatgca tcctttaccc ccgaataacc atagattata cctccggttg ggaaatcggg
    15361 agctttgatg tgtttcatca gctctgaaat ctcgatttcg ttattgtcga tgtaagcaat
    15421 acaggcatcg acggtttcgg aaagattgtg tgtggccata ttggtagcca tacccacagc
    15481 aatacccgac gaaccgttta caatcagatt ggggatgcgt gatggaagaa cagtcggttc
    15541 tttcagcgta tcgtcaaagt tgggttgaaa gtctacggtt tctttgtcaa tgtcggccag
    15601 catttcttct gcaattttct gcaaccgtgc ttcggtgtat cgcatcgctg ctgctccgtc
    15661 accgtcaaca gatccgaagt taccctgtcc gtcaaccatt gtatagcgta ttgcccaggg
    15721 ttgagccagg cgtaccagcg tgccgtagat ggaggagtct ccgtgcgggt gaaacttacc
    15781 cattacttcc cctacgattc tggctgattt tttataaggt ttgtctgaat tgttgcctaa
    15841 ttcgttcatc ccgaataaaa cacggcggtg taccggtttt aaaccgtccc gaacatcggg
    15901 taaagcgcgg gatactatta ccgacataga atagtcgatg taagatgact tcatctcgtc
    15961 atcgatgttc actttaataa ttctgtcttc ttgatctgtc atctgtcatc tgtcttttat
    16021 tttataacta aaaaattatg cgtcacacag tttgttgctt ttctaaatct cctgaaatca
    16081 ggtgtttgtg actgttaatt gtgtgacgaa aagaatttat accggacata agttttgccc
    16141 ctgaaaaatt gcactaaatt actacttttt tgttaaatac acaaatggtt tttcaacaaa
    16201 aaaagaatac aatatacact gtattctttc catggagttt cagttttttg ctctgaatca
    16261 ggcttctttc ccctttttgc tgtgaggaca aaatctgttg tagatcagcg tgtccagagc
    16321 aattttgccc ggtcctgtga gtaatagtac cacatacatg gcaaggtata gcaggggaag
    16381 ttcacgtccg ttgaacggag ctccggcatg tgctacaaat gcagctaccg acataccgat
    16441 aatgagtgga attacagcaa gccgggtcag aaatccgaat aaaagtgcaa tggagcaaaa
    16501 gaattcggca aaaataatca gggaaaggcc aagtgccggg ctcataccga gcattgcagg
    16561 aaatccctga ctcatttcgc taaagtttga tagctttgcc cagccgtgtg tgagcataaa
    16621 tatacccagt attaaacgta ataccagtaa tccgtaatct ttcagttcaa aacagcaatt
    16681 tttattgctg caactttcag ttttcttttc aatcataatt tcttgttgtt tttgtttgct
    16741 catttaacat tcacaaccag aaaaagttta tactatgctg tgtgaaaata gataattatt
    16801 tttaattccg ggacctattg catttcggtg aggtatttcc agtaacgtat tggcatatgc
    16861 tgtcgttgca acctgatatt tatccgttct ttaattgcaa ttgttttgaa gtattgtttc
    16921 cacatcttct ggaaaagcag ttcctgatcg tcatatttgc cagtgtcggg tttaccgtct
    16981 tcattcacag gagaaatatt atcggtaaat gtgatttctg ttacggtgtg cagatcgtaa
    17041 tacaacccga atttgcgttt tgtatcgtaa ataatccatc gctggtcggc ataccggttt
    17101 cgaaaatgtt cgactgtcat tgctaaagtg ttgtactcag gtgcaatggg tgcgaaataa
    17161 ataccgtctt tggtcttttg gaaacggaca aactggatta cccggtgaac agaactgttc
    17221 acttttttgt aaatctctgt gcatttcaaa acgtcttcgt cgccaaaatt gacttcgata
    17281 gacacggaag agttgaaagc ttttgtcatg taacggaaca acaaaagatc tgcttcggga
    17341 agttccgaca tccagacagt aagtatcatc cgttgggcag aggcggatat tttcttttca
    17401 agccctttcc acacgcgggc tgatttgtct gtttcggtga tgatgtgaaa tgatttgtcc
    17461 agaaaaagtg gtttcacttc atggcttcca agcagaatgt caggcgtttc tttcgcttcg
    17521 aaagcaaaga aaatacagca aagtaatccc tcgaacgttt tgtcgtaagt gaaactggtc
    17581 atttttcagt tagtattttg tcttatttta gtattctgtt gtttgttgaa aattctcagg
    17641 atttattccc cccagtcgaa ggcaagctgc agggtgtcga tttttgtgtt tttgttttca
    17701 gtaaataatt tacggagata tccgggttgt aattcctgta ctgttgaaac cggcaactcg
    17761 cgagaaacaa tgaaatattt agcgcgcttc atcacaactc caatctttcg gagttgttca
    17821 ttgcccaatc gtgaaaatct ccgggcggca attatttttt ttgccgatag aactccaaca
    17881 ccgggtatcc gcagaatagc ctcataagga gcagtattaa catcaatcgg gaaaaattga
    17941 ggattgcgga ttgcccacgc cagcttcgga tcgatttcca gatcaagatt tggatttttg
    18001 tcatccagaa tttcatcgga actgaagtag taaaacctca tcagccagtc agcctggtac
    18061 aaacgatttt cgcgaaccag gggaggctgt ttcagcatgg gcagacggct gtcgttgttg
    18121 atgggaatat aacccgagta gtacacacgc ttcatgcttt cgatggtgta aagtcttgac
    18181 gaaagtgaaa gaatctgccg gtctgtttcg ggtgaagcac caacaatcat ttgtgtactc
    18241 tgacccgcag ggacaaattt aggagctgaa cggtatttct tcctgtcttc ctgattttca
    18301 agtattccgg ttttgatgta tttcatgggg ccataaacac tcttataatc tttttctgga
    18361 gcaattattt tcagactttc ttccgaagga atttcaatgt ttacgctaag cctgtcaacg
    18421 tacataccgg ctttatttac caatgcctga ctggccccgg ggatgctttt catgtgaata
    18481 taaccgttga atttgtgcac ctcccgaagt tctttcacta cagacaacat ttgttccatg
    18541 gtgtaatcgg gggtacggat gacgccggag cttagaaaca atccttcaat gtaatttctc
    18601 cgataaaatt cgatggtaag ttccgccagt tctttgggcg taaaggttgt ccgtctgatg
    18661 tcgttcgacc gtcggttggc gcaataggca cagtcgtaga tgcagttgtt tgtcagtaat
    18721 atcttgaaaa gcgaaatgca tcgtccatcg gctgtaaagc tatgacaaat gccccatccc
    18781 gatgtagttc ccagtgtttt ccccttgttg tcacgggtag ttccgcttga tgcgcacgaa
    18841 acatcgtact tggcggcatc ggcaagaatt ttcagttttt cgagagtcgt ttcgctaatc
    18901 atggggcaaa gaaagcaatt aaaatcctgt aattttattt gcagatctta aatttttccg
    18961 gatgttgaaa actttttatc cggctgcttt cttttcagtt tgtgtaatta ttccagttcg
    19021 ccgcatgcaa caaagcagtt ttcaaaacga tcccgatatt cgggtatttc gccaaaaata
    19081 ccatacaccg aagagcctga accagacatt gaagcataaa ttgcccccat ttgataaagt
    19141 tcgcttttaa ttgcagcgat agcgggaaat ttttcgaata ctgatttttc aaaatcattt
    19201 ttaatgtcgt gtttccagtc ggctattggt ttccggatta gttcggtaag gggtgaagcc
    19261 gagggttgcg gagtgatgcc cgcatatgct tctgctgttg atacatgtat gtcgggtttg
    19321 acaagtagca aaatatatcc tttcagagat acatcagcgg gtgcaaagat gttgcctgta
    19381 ccggttgcgt aaaccggttt gttgcggatg aaaaacggac agtccgaacc gagttttgaa
    19441 gctaactgtt ccagatgctt cggagtgatt ttcagtttga attcctcatt cagggctttc
    19501 agcatgaagg cagcatccga tgagcctcct cccagacccg cgccgaatgg aatttgtttc
    19561 agcagactga tatcaacagg cggaaactga tatttggagc gtagtaaccg gtaggatttt
    19621 acaatcaaat tgtcctcagc atcgccaccg atatttatac cagataccga gaaactgtaa
    19681 tcgctgcacg tatctgatgt ctctacactc aatacatcgt gaagtcctat cgggtaaaag
    19741 atggtttcga tattatgata accgtccggg cgtttttcga caacattcag acccagattt
    19801 attttcgcgt tcggataaac tagcatggct tataagatga taaatttaat acttcaaaga
    19861 tacagttttt gtgaggaaaa ataaaagcaa aaacagaatg aaaatagaat tgtcatttag
    19921 taaaaaagtc agttgttcat ttcttgttga aaggtttatt tggtacggag gataatcttt
    19981 tgtacttttg aaattccaaa attgaagaca aataatggca gaaaaaagaa attataataa
    20041 gaaacccgca tccgctacac cggttcctgt caacgaattc ggaaaacttc ctcctcaggc
    20101 gcccgaactt gaagaggaca tccttggagc catcatgctc gaaaaagatg catatgcctc
    20161 tattgccgat atactgaaac cggatttttt ctataagaca gttcatcaga aaatctatga
    20221 agccatcgtt gacctggcat tgcatcagaa cccgattgac atgcataccg tcaccgaaat
    20281 gctccgtaag aagggaacac tcgatgatgt aggcggtccc tattacatca ccttgctgac
    20341 ttcaagggtt tcttcggctg ctcacctcat ttatcactcg caaattatcg ttcagaaata
    20401 cctggcacgc gagcttatcc gcatgtccag cgatattcag acaaaagcat tcgacgaaaa
    20461 gatggatgtg gatgacctga tgcaggaaac ggaagctaaa ctttttgaga ttacacaaca
    20521 gaacctgaaa aaagatgtcg ttcagattaa tccggtaatc gaggaagccc gcaaacgtat
    20581 gaaggaagcc gccaaccgcg acggtgcaag cggaattcca tccggtttca ttgagctgga
    20641 taaaatcact tccggctggc aaaaatcgga ccttatcatt cttgccgcgc gaccagctat
    20701 gggtaaaaca gcttttgtac tttcaatgac caagaacatg gctgttgact ataatatgcc
    20761 ggtggctttg ttttcactcg aaatgtcaaa cgttcagcta gtaaatcgtt tgctgatgaa
    20821 cgtgtgcagc ctcgaaggtg agaaaatcaa gaatggggaa ctttcaaaag aagaatggaa
    20881 aaaatttgac aaggatgtta ccgcgcttta tgatgctccg atttatgtgg atgatacacc
    20941 aagtttgtcc atttttgaac tccggagtaa agcccggcgt ctggtcaaag agcatcatat
    21001 ccagtgtatt atcattgact acctgcagct gatgaatgcc agcggtatgt cattcggtag
    21061 ccgcgaacag gaggtaagca ttatttcacg ttcactcaaa ggtcttgcga aggaactcga
    21121 tatgccgatt atcgctctgt ctcagttgaa tcgtggattg gaaagccgtg ccagcaatca
    21181 aaacaccgac ggaaaacgtc cgcagttatc ggacttgcgt gagtccggcg ccattgagca
    21241 ggacgcggac atggtttgct ttattcatcg gcctgagtat tatggcatga aagtggataa
    21301 agatggaaac tcaacagaag gtatagcgga actcattatt gcaaaacaca gaaacggggc
    21361 agtgggtgat gtgcggctga agtttaagaa tgtatatgct aaatttatca atctgggaga
    21421 tgaagcagat ccgtttgccg gtcagggata tgctacattc aaatcgaaac tgaatagtga
    21481 tgaaatgaca ccagatttct atgcaacccc cgaaatgaac ggaaatcctg attacctcaa
    21541 accagcacct attggtggga gtgtgaacaa caacgacgtt ccgttttaag aatccgggat
    21601 ataggataaa aagaaacagc attaaatcat cggtttaatg ctgtttcttt ttcttgtaaa
    21661 attaatgttt gaaattttct gtctctttgg gattatgttt gtatttgaaa aacacgacaa
    21721 aaagtactgc tacaaccaat gcataagcag aaaaagttaa ccagatgcct tgccagtctt
    21781 ttaccagtat ttctttgccc tcaatgattg ccgggtgctc ataccatttg gcaatcacca
    21841 gaccggcgac gatatttcct ataacagccc caactccatt ggtcatcagc atgaaaactc
    21901 cctgtgccga ggattgtatt tttgaatcgg tgtttttttc tacaaacaaa gcgcctgaaa
    21961 tattgaaaaa atcgaaagcc ataccataaa tgatgcagga tgcaataatc agaataaatc
    22021 cgggtatgga attctgagct attccaaaca gaccgaaacg cagcacccag gctaccatac
    22081 tgaaaagcat tactttcttg ataccaaatt ttttcataaa gaaagggatg gcaagaataa
    22141 acagcgtttc ggaaatttgt gaaacggaca gtatgatcgt ggaaaagtta ttgataagtt
    22201 cacctttggg gaacacgttt gcatcctgca aaaaactgtc gccgtaagca ttggtcagct
    22261 gaagggcagc gccaagcagt aaactgaaaa tgaagaaaag agccattttc ttctgtttga
    22321 acaggataaa tgcttccagt ccaagttttt gagccagtgt ttttttaaca gttacagttt
    22381 tctcttcatt tctgaccgat ttaggaagaa aaataaaaga aaacaacgac aaaatgaagg
    22441 cgaaaatagc tgaaattata aaggatactt ttatactctg gccgaatccc cattctttgg
    22501 taaatacatt tgtaatccac atggcggcaa taaaaccgac agttccccat acacgaatcg
    22561 gggggaaata ggttgtggga tctttgccct cattcttcag caccgtaaaa ccgatggagt
    22621 tgttcagacc gatggtaggc atataaaaac acattgcaat aaagagaacc cagaaaaacg
    22681 gaaggggagc atcgatgagc ggcaggaaac acatggtcag tccaaacagc agatgcagca
    22741 gggcgtaaac gtaatttgcc ttccatttgt cggcaataat accaaaaagt gtgggcatga
    22801 tcaacgaagc aaaacccatg gtagagaaca caagaccaaa ctcggctccg ttccattgtt
    22861 tttcgacaaa tccgtaatgc ccgagggtca tcatccaggc tccccaaacg aagaactgga
    22921 ggaaattcat gataataagt ctaaatttca aattcatata tgtgtatttt gtgtatttct
    22981 tatgtctttt ttctgttaat gtttctttct gtattctatc tcgtcacgga tcatggcggc
    23041 gatttcgtat cgttcttcag cgagcgcctt ttcgaggaaa aatttcagtt tttcgtccgt
    23101 cagcttaacc agaatttcgt ttgtgaggcg gtcaaggctg atagattccg atatttctgt
    23161 ttccagttcg gcgtcctgtt ccgtatcgat ggccgtccct acaatgtcca gaatatccga
    23221 gttgatataa atgtgcgcgt cggttcgaac tgccagcgcg acagcatcgg aggttcgggc
    23281 atcaactaca atggttttat tgtcgtgaag caggtatatt tccgaataaa aaatatcatt
    23341 gaccatgtcg taaatcacca ctttggtaag tttagcttcc agcatattga tgatggaatt
    23401 catcaggtcg tgcgaaagcg gccggggcga tttggcatta ttcagcttca gtgcgatgga
    23461 ctgggcttcc gcttcgccta tcagaaccga aaaacgcctt ttctgacctg tatcttcggc
    23521 taaaactaac gaataggttc ctccaagtga ctgattataa accattccgc tgatatgtag
    23581 ctttatttca tgtgtcattt accggtagat tttgagttat gttaattttt aattgaacag
    23641 caaatatact taatttttaa tgaaaaaata attttgatat gaaatttggg tacatggcgt
    23701 gatttattca ctgattaaca aaaaaatagc tggcaaatag gaaaatatat gtaattttgc
    23761 cgcttgaaaa gatgaaaacg acaggcatac tcaaacagtt gattatacgt acatggaagc
    23821 ttacggccga tacaacagcg gcatgggatg agatagcagc tgagaaaagc accgtgaaag
    23881 atgtttttct gcaatataca atacctttta ttttaatttg tactattatc agcacgttat
    23941 tcggcttttt gtacgctgat gaaaaaaagc ttacaatcgg aattctgaaa ggaataacta
    24001 ttgcagtttc tttactggca gggaatttgc taacttactc ggtaagcatc cgtaccttgt
    24061 cgaagtatct tcctcatttt gcagtgaagg aaaatgtagc ccggttggtt gtctattcat
    24121 tcagtctgat gtactttttc aggattgtaa cagctatttt acccgg
//
LOCUS       DAOPYB010000028        23521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2170_length_23521_cov_9.189253, whole genome shotgun sequence.
ACCESSION   DAOPYB010000028 DAOPYB010000000
VERSION     DAOPYB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 23521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23521
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2170_length_23521_cov_9.189253"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKH58_03795"
     CDS             complement(<1..379)
                     /locus_tag="PKH58_03795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX88181.1"
                     /translation="MRRLKQMLSDRNRKKKFELALSLIKPNDTIIDIGVDPWLGGNTN
                     YFEKWFNLPNQLTCLGVNSDFSQFRQEFPNFKLIEFNGTDFPMFERKFDFAFSNAVIE
                     HVGDKDKQVLWLTEVAKICNTLLI"
     gene            complement(595..1029)
                     /locus_tag="PKH58_03800"
     CDS             complement(595..1029)
                     /locus_tag="PKH58_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding protein"
                     /protein_id="HNX88182.1"
                     /translation="MKKISYIIILVAAIIMASCEQNIDVNNIQANKAEGEWVIKAYIN
                     GTNVFGSFPIRTYSTISGKDSLNINEYEGLFWDFNVKAAVTAENTFQTQHSVNAVSGK
                     KIGIKVQNGRIINNDSIYMEVQFEDDETPYGITYQLAGHRTK"
     gene            complement(1050..3707)
                     /locus_tag="PKH58_03805"
     CDS             complement(1050..3707)
                     /locus_tag="PKH58_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX88183.1"
                     /translation="MKRKQLMKKISVLFMLIFSTLPFLTYGQGSTYTGSYTPSSPLKY
                     NSKNDIIIEGIEINNATTSSILLEACNNVIIRNCRFANSPGYVAIDLVDCSNITITNC
                     EFESVATGVYALGCRGNIKFMNNELKNANPNNLPNYGSIIYLNQCFGAGNEISYNIME
                     NIPGQSNTIDCLSMYMSNGTPESPIKVTNNWIRGGGPDPSGGGIMCADYGGSYYIIEN
                     NILVDPGQYGIAVASGHHITVRNNKIYGKQQSFTNVGLYAWNQSGDECHDLTIENNKI
                     NYTNRNGQISTYWFDNNVLPILGAGPTTNVYDPTVTASILPAQIIGRARMNSAPTYVW
                     NKTTTASYAVASNWTPTRTSPSSKDILQFSNGGSVVVTNMPSEAIDQLELLNNTTVEI
                     RSASSSVLTVNSLIINAGSTLKIGSGNQMTVVASLTNNGSLNLLSDNTKGTASLLTPA
                     TITGTGAYVVQQYLTSGLNCHISSPVTSAQASTFTTATNTTTYNEPTSKWVNVTGKLT
                     PLRGYLSNMTITNGNVIFNGLINNGNLSVPLTRTAGQLNAGFNMLGNPYPSYLNLNNL
                     TNSDIETTIWYKSKDASSLFDVYNTKSGVYTSNSGLPVTGVVAPMQAFWVRVKKGKTA
                     STLSLTNALRTHRDVTGNVFRAPTVNYSNLLLIRLQVSNGTYADETVIYSDGSASNGY
                     DDYDSSKGMNSTTSLVPDLYTTVDGENLAINGMNALPLDTEIPISFAANQSTSTSFTL
                     KATEINNLASGISLIIKDMETGNEANLTDGSTVYTFTASTGSTKNLSLILRTTSVTTG
                     INTGNGTLVDEQATTIYKNSRNQISVNCNGGVASDATVSVFNTAGQKLVFQRIKSYHT
                     VLETPFTSGEYDVTVNNGLKIKTKRISVY"
     gene            complement(4531..5091)
                     /locus_tag="PKH58_03810"
     CDS             complement(4531..5091)
                     /locus_tag="PKH58_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX88184.1"
                     /translation="MKLYIKYTLYVQCKKEMLEQLDQLGLHYGELEKGELEITEDVDY
                     DQIVKLKTNLVNSGLELVDDRKSNLVQKIKNVITETIQNTDVQLKTNFSVYLSEKLNY
                     DYTYLSTLFSEMTGVTIEQYLISQRIERVKELLIYDEMNLTQISYLLNYSSVAHLSNQ
                     FKKVTGLTPSFFKYMKQRKMIIRDIG"
     gene            complement(5275..6258)
                     /locus_tag="PKH58_03815"
     CDS             complement(5275..6258)
                     /locus_tag="PKH58_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX88185.1"
                     /translation="MKKVILTIINALFLSISVTLCQGVTYQGTYTPSSRIEINGRKNF
                     IIDGKEIKSVKNHCITLWACHNVIIRNCKFGPSSAFVGIYLCDCSNVKITNCIFEDVA
                     TALLAQGCSGNIKFENNEVKNVNSKKLRNYGSMVLFNEVRGVGNSICYNICENIAGES
                     FPGDIISLYKSNGTSKSPIKVSNNWIRGGGPADWGGGIMLGDCGGSYFLVENNILVDP
                     GQYGIAVASGHHITVRYNKIYARRQEFCNVGLYAWNQTETECHDLTIAHNEINYTSKT
                     GDRGILWFAENVGKVAGTETNIFNPQLKSSILPAKIIGRKSGVANTKKSVN"
     gene            6617..7438
                     /locus_tag="PKH58_03820"
     CDS             6617..7438
                     /locus_tag="PKH58_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX88186.1"
                     /translation="MKKACHLSYIFLISLFISSCSQVKDIAYFQNTDEKAITSQIQSR
                     SESLFDPIIKPKDLLSISVVSTEPGASRIYNLITPQTNGSSGENSIISQPALQNYLVD
                     NDGFINFPVLGKIQIKGLTTKELGVKIQDMIAPAFTKELPIITVRIINYSVNILGEVI
                     KPGKYETTNERLTIFEGLALAGDMTIYGRRDNVKVLREDAYGNIKYITLNLNDKNIIN
                     SPAYFLEQNDVVYVEPNKSRSESSKFGSAENFRISTISVLFSVVNIAATLYSAIK"
     gene            7500..9938
                     /locus_tag="PKH58_03825"
     CDS             7500..9938
                     /locus_tag="PKH58_03825"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNX88187.1"
                     /translation="MNKYPEKEDDYNPLTEKPVDFIGLLIKYISYWKWFILSLIICIA
                     IAAIYLRYTLPKYEVKTSVLFKDDSKGSGSVEMNVFNDMSIITQKNNIDNILELLKNS
                     VIIEKTVTELGLYTSYTSINTIPLIQKTGINRYFPNFGRYREKVLYGDECPVLISLPD
                     STLKYFTGQVVFEVLMHPYGLYEFSGTYAEKKFNIKASFSDKKVMLPFGVVNIKRGNI
                     KLSKDMLIEVAIHQPSNVANYFLSQMKMVLASKTGSVVDLSFICSNENLGKDFIRKFI
                     EVYNREEMNEKIEMANKTSQLIEEHLNALSSELSSVESQAEKYKQSQGITDLASQSDL
                     FNNQTADVEQKRLEIETQLAIVSDLNNYIQNSANRDQLIPSNSGINSGNLNNLVSDYN
                     RLILERNRLSRIASGSNQAMIDLTNQIETMYNTVQSSMQNERKNLQIARRDLLSKYNQ
                     TSARIRAIPRQERQYTEIKRQQGVREELFLYLLQKKEEKYMNMSSVVPSSKLIDNVHS
                     LGPVSPRKNIILLSSLLIGLLIPVIGIKVRDLLRYQIENKEELEEISIVPVLGEIPRT
                     DQSGKVLVKENNTDIFTEMVRLLRANLLFVMDNPDKKVINMLSSVSGEGKTFVTINLA
                     ISLALLDKKVLIIELDVRKPRLAEYIGIENKSGITLFLSGQINQNELIKPTDIHPNLS
                     IITAGTIPPNPNELLAKPALDMLIDDLRHQFDYIVIDTAPIGVVSDSFLLNRLTDVTL
                     YVVRAEFTPKKDIEDASLLYKKQKLKNMYFILNDVDLNKNTYRYGYGKKYGYGYVYGK
                     NKHTYGYGTKTEKA"
     gene            9979..10386
                     /locus_tag="PKH58_03830"
     CDS             9979..10386
                     /locus_tag="PKH58_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HNX88188.1"
                     /translation="MLPLKGARIIDLPKTLDRRGNLSVIEEIKNIPFKIKRTYWIYDV
                     PGGEIRGGHAYKANKEFIVALSGSFDVILDDGKERKVFSLNRSYYGLYVPKGVWRQMQ
                     NFSTNSLALILASAKYNPDDYIFDYELFKNSII"
     gene            10383..10799
                     /locus_tag="PKH58_03835"
     CDS             10383..10799
                     /locus_tag="PKH58_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HNX88189.1"
                     /translation="MKKTTVYDCSIIEIDKHHHEKGNLSVIENGKTIPFGVKRVYYLY
                     DVPGGESRGGHAHKELRQLIIAASGSFDVKLDDGSQKRTYTINRPYEGILVVPGIWRE
                     LDNFSSGSVCLVLASTEYEADDYIRDYNEFIAYKKI"
     gene            10796..11320
                     /locus_tag="PKH58_03840"
     CDS             10796..11320
                     /locus_tag="PKH58_03840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX88190.1"
                     /translation="MIHPLSDVLSTNIGENTKIWQFCIVLPDAEIGENCNICAHVLIE
                     NKVKIGNNVTIKSGVQLYDGVTLEDYVFIGPNVTFTNEKVPRSKTYDNSKIKNTLIKK
                     GASIGGNATVIPGITIGEYALVGAGSVITKDIEKNTVWYGNPARKKGYITDDGILLDI
                     NKKDKDGKYYELNL"
     gene            11323..12423
                     /locus_tag="PKH58_03845"
     CDS             11323..12423
                     /locus_tag="PKH58_03845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNX88191.1"
                     /translation="MIKFLDLQKITQKYSVEIHEAVSGVINSGWYLTGAENLKFETSY
                     AQFIGTKYCIGVANGLDALRLILRAYIEMGIMQAGDEIIVPANTFIASILAITDNGLI
                     PVLVEPDINTYQTDNSKIEEAITPKTKGLMIVHLYGQCAYTEKIASVCKQHNLKLIED
                     NAQAHGCKFNGITTGSLGDAAGHSFYPGKNLGAFGDAGAVTTSDEKLAEMVRILANYG
                     SSHKYIFEYQGLNSRLDEIQAAILSVKLKHLNEDNERRKEIAKFYIENIKNPTVITPK
                     VNDWNANVFHIFPIRCRRRDDLQRYLADNNIQTIIHYPVPPHKQACYKEWNQLSLPVT
                     EQIHREELSLPISPVLTDEEAEMVVRFVNSFR"
     gene            12423..13124
                     /locus_tag="PKH58_03850"
     CDS             12423..13124
                     /locus_tag="PKH58_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88192.1"
                     /translation="MKKNIYIFLRKFAKKTLTFPYSVFTILVCKIRSIYFSSKIDDGG
                     GKIIISDTFLDFKINKHPTARLIIKGNLNTIKHVGNKNPIYIELGENSTLSIEGDFTI
                     GRGVRITLYTNSELIIGGREKESDSGITADTLIMVYKKISLGKDFMCAWNVFISDSDW
                     HHIEGQSHHSDIIIGDHVWIANSNNILKGSVIGNNTIVASNSKTINSVYPDNVLIGGV
                     PAKILREKIGWNRDV"
     gene            13152..14642
                     /locus_tag="PKH58_03855"
     CDS             13152..14642
                     /locus_tag="PKH58_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen translocase"
                     /protein_id="HNX88193.1"
                     /translation="MHEQQSSYRQIMKATSLFGGVQVFQILISVIRSKFVAVLLGPEG
                     MGIVGLLTTTTGFIGALTNFGLSTSAVKDISESNTSGDQNRISGVISVMRRLAWITGL
                     LGAIVTLILSPWLSQLTFGNKDYTLAFIWISVTLLFNQLSSGQLVILQGLRKLQYLAK
                     ANLYGSLVGLVIVVPLYYKFGIRGIVPVIIVTSLISLFFSWYYSRKVEIKPMQISKAK
                     TISEGKNMLTMGFVISLSGLMTMATAYLLRIYINRTGNVADVGLYSAGFTMIGTYVGM
                     IFTAMGTDYYPRLSAVAKSNELSRQAINQQAEIAILIIAPLLIAFLVFINWFVILLYS
                     NQFVAINGMLYWASLGMFFKAASWAIGFIFLAKSASKIYFWSELVAETYSLIFNIIGY
                     HFWGLNGLGFSFLAGYVIVFVQVYIIAKINYQFSFTRSFSLIFTIEFLLAMLSFTAVK
                     FLNQPYPYIVGSILIVLSAWYSFRELEKRIGIKELIQGFLQKNIKN"
     gene            14656..15591
                     /locus_tag="PKH58_03860"
     CDS             14656..15591
                     /locus_tag="PKH58_03860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX88194.1"
                     /translation="MKEPKKPLISIVVITFNSSKYVLETLESAKAQTYQNIELIVTDD
                     CSVDNTLSVCTEWIKKNKNRFTRTEIISVEKNTGIVPNLNRGLNAVKGEWIKFIAGDD
                     RLNDNCIERFYEEVYQNSEIEVIFSNVSVNGIDIPEDYLPKFFLLETKEQYLNLLKGN
                     SLPAPAFFIKSTTLKQLNGFDEQFPFLDDYPLFIKILKSGRKLYYVNDFLVYYRDNND
                     SVSHSKVMNINYVKSLKLFYKEVLLPELTANKMYFYALHYRLEYLLLFFSEHELIKYK
                     KDYFRLLNIFSALIWKAKLGKLQRNFYNKLLLFIS"
     gene            15673..17019
                     /locus_tag="PKH58_03865"
     CDS             15673..17019
                     /locus_tag="PKH58_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88195.1"
                     /translation="MLKTSNILFLSDNEERTDEESTVASLKSSYDTKLRRVVNYLPLF
                     FLLCFSGNPIISSMGYSKMLLVLYSFAFIVYVFSFVNFEYIKLKINQISPVIVFIVLL
                     VIYQHFLLGKVSYPGVIVLVLKIFLGLFTFIFYQAKRLSFVKTFIKIMAFLAIISLPF
                     FILNQFIQIGIQLDNIFLKSLILYTSYPAETFSESLLVRNPGMFWEPGAFAGYLILAL
                     LFVILENRSFTIGNYKKEVFGILLGLITSQSTTGYFLLGILILLFIWNRYSWGKIIIV
                     PITLLLVYWGYNNIAFMGNKVENQFSNAMAMGESDVSNTRFGALNMDLMYIRSQPLTG
                     NGLDISTRYRFHPWVKEDIGHGNGMSNFAAIWGIPFFLYFLYCVYQFAHHYSRKKLIA
                     IVFLLTIVLVLQGEQFLNYPMFLSFFSLPVFYRQFSESRKGYLLKEDVKTDLNINL"
     gene            17016..18281
                     /locus_tag="PKH58_03870"
     CDS             17016..18281
                     /locus_tag="PKH58_03870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX88196.1"
                     /translation="MKVLWFANTPCGATEKLIPDHYLGGWLKSLEQQLVLNNDIELTV
                     CFYWEEAIEPFRYKNTMYYPVFRPGRNSKTGRLINRILRRDNDKKEIGELVKVVNTVR
                     PDIIHIHGTEHNYGLILAHTDIPAVISIQGLLSPFSEMYFSGVPYCTAIRFEGIKTKL
                     LFRSYSRTIAELKKRATKEREILSHTQFVIGRTDWDRRITGLLSPSSQYFICNEMLRP
                     AFYQFTWHKSLFNNPLQIVTIMNSDLSKGLETVIKSAEILCLQNSVTFKWIIIGLKET
                     DELPMMLKRWLNIDYQSLNISFYGLKSETEVVEILVNSDIYCQVSHLENSSNSLCEAM
                     LIGMPVIASFAGGTDTIIENNKEGILFQNGDAYSLAATITELSKNFEKAVKYGTNARN
                     IALKRHNPNEIVSKQIEIYHSVIQTTEKK"
     gene            18403..19602
                     /locus_tag="PKH58_03875"
     CDS             18403..19602
                     /locus_tag="PKH58_03875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX88197.1"
                     /translation="MAQIFCYDDNGDKGFFQHYYRIGRAERTCGSLFFRLKTHEIPKS
                     GFDIIKPPSEIRKHGLTEKIKLQAKRYLVDTGIVELIFRPKMSSEMKTWIDEFNPQVI
                     FVQGYNLYFVWLAMLLKKMTGAKLAFLTTDDWPSYLYSGLYEEPTLFRWLMRPIVKSA
                     TQRLMNNVDIPFAFNSIMSEEYKSRYNKKFITINHADEAESFKNAVPQRYHPDNVVSI
                     VTTGYFNKYRWPLLLDVDECCRKLTEKGNPIRLLVISSGMEKEGIAALRNAKFTDIIP
                     DPGNELLRGYLKAADINLLPEAFDPQVAAAISLSVSTKAHLFMLSQHPIIVYGHPNTG
                     VVKYAKSEGWGSVVSKRDLSLLTNVIEKLIINKDEAKRLISCSKNTFEKYHSLSKNQK
                     KLLEVLQ"
     gene            19632..20300
                     /locus_tag="PKH58_03880"
     CDS             19632..20300
                     /locus_tag="PKH58_03880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX88198.1"
                     /translation="MKKRLIKIMKKSNGMLYIMAAIYSLRSFLLIKGCKNNSIQLRGA
                     FLKNTRIRIDGTNNIVKIAHENRLNNCLIYIKGNNCEIFIEKHCILANIELWIEDDNG
                     KITIDYRTTAEGGHIAATEGKSIYIGKDCMFSHDIEILNGDSHAIYMLENKLRINASK
                     SVYIGNHVWLGSSVKVLKGSTIEDGTVVSAGAIVTGHLESNSIYAGIPAKKIRENIFW
                     ERER"
     gene            20300..21430
                     /locus_tag="PKH58_03885"
     CDS             20300..21430
                     /locus_tag="PKH58_03885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX88199.1"
                     /translation="MEKKKLLMFHPALAPYRIDEFNALNCLYELKIVFLSENLWNQKF
                     NQSRLLSQLTFRYSYLLKGIGFKERIFRFGMLRTIREFKPDIILGYEFSPVTQYLIIL
                     KRLGIISQKIGSITDDSIDICSHIQSTFRSTARKIALKHLDFLVVMSQEVSAYYQKEF
                     HLNKNQLIIAPIIQDPERLRKNSKTLEKTASEYLQRFQLAEKKILLFVGRLVQEKGLT
                     IFLETIHPLLLENNQILVVMVGEGDELKKLQSMTEQKHLNDRIIFPGRYEGDELYAWY
                     LCASGFVLPSTYEPFGAVVNEALIFGQKVLCSQYAGASSLIRADNGLIYNPLNKEESI
                     EKIKLFINDIETVRDVSLSKKPSLITNHQKEFIEEWEKTGLL"
     gene            21438..22541
                     /locus_tag="PKH58_03890"
     CDS             21438..22541
                     /locus_tag="PKH58_03890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX88200.1"
                     /translation="MAEKKNILFILHLPPPVHGSSMVGKSIMDSELINSSFECRYVNL
                     LMSRTINETGKPGLIKVLRFMRVWFSVLGEIIRKKPDLCYLALTASGTAFYKDFLLVL
                     LLRIFRIKHIYHLHNKGVKQNQTSKLNNQLYRYVFSKAETILLSKQLYSDVESFVKES
                     GVYFCPNGIKDTVMPALPRSCPENSAIKLLFLSNLMKSKGVYLLLDACSILMHKNLDF
                     ECNFVGGEGDISFDNFNKYLIDKGLNQNAKYLGLKFGAEKHKAFREADIFVHPTSNDC
                     FPLVLLEAMQNQLPIISTFEGGIPDIVENDKTGFLIPANNAGALAEKLEILIKNPEMR
                     QKMGSAGRLKFEKEFTLEIFEKNLHEILYKVSE"
     gene            22585..23520
                     /locus_tag="PKH58_03895"
     CDS             22585..23520
                     /locus_tag="PKH58_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88201.1"
                     /translation="MVTEVSKELYRQHFHQDPHPYISEEFIDLVKNKTDKVVRLIESD
                     YNVSIGLLGGIKDGILYSPFSAPYGGFHFINDEVLYEDIFRFLTHLKDYVIAEKLKNV
                     HITLPPDIYCSSMNAKFVNSFVRLGYIMKTPEIVNWIDLKSFDGKWGKKAIDKNIKKT
                     IQNKLSFNPVSDEKQKNEAYEIIYDNRTLQGREIYMNLTDLENVNNIFPVDYFLVKNV
                     NNENLASAIFYRGHNKIIQAVFWGDIVPRIQVGVMDFLVMNLFFFYKNLGYEYICLGR
                     SSAEGIPNEGLLRFKEAHNCTSSLRYSFTWGPERV"
BASE COUNT         7713 a         4170 c         4297 g         7341 t
ORIGIN      
        1 ttatcaataa cgtattgcat atcttcgcaa cctccgttaa ccacaatact tgtttgtctt
       61 tatcacccac atgttcaatg acagcattac taaatgcaaa atcaaatttc ctttcgaaca
      121 tggggaaatc cgttccattg aattcaataa gtttgaaatt gggaaattcc tgtctgaatt
      181 gggagaaatc tgaatttacc cccaggcatg tgagttgatt tggtaggtta aaccatttct
      241 caaaataatt tgtatttcct cctaaccagg gatcaacgcc aatatctata atggtatcat
      301 tgggctttat cagcgatagc gcaagttcga actttttctt tctattccta tccgaaagca
      361 tttgttttaa tcttctcatc gaccaggcat ttaaatccgt gaatattagg tgtataatct
      421 gatttttcaa tatcaatttc aaaaataatt atttttttac acaaatcact tcattgacaa
      481 aataatttat ctcaaggtaa aacaaacagc ccccaaatca cctataaggt ttttgggggc
      541 tgaaaattta tataaactgc taatttataa tttattttcc cgttatttta aagtttattt
      601 ggttcggtgg ccggcaagct gataagttat tccataagga gtttcatcat cctcaaactg
      661 aacttccata taaatactgt cattgttgat aatcctgcca ttctgcacct taattcctat
      721 ttttttacct gaaacggcat tcactgaatg ctgtgtctga aatgtatttt cggcagtaac
      781 agcggctttc acgttgaaat cccaaaacag tccttcatat tcgttaatat taagagagtc
      841 tttccctgag atagttgaat atgttctgat aggaaatgaa ccaaaaacat tagttccgtt
      901 gatgtaagct tttatcaccc attcaccttc agctttattt gcctgaatgt tattcacatc
      961 tatattctgt tcgcatgatg ccatgataat ggctgccact aatataatta tataacttat
     1021 ttttttcatc tgaaatgatt atctttctat taatatactg atattctttt tgtttttatt
     1081 tttaacccgt tattcacagt cacgtcatat tctcccgaag tgaatggagt ttctaacact
     1141 gtgtggtaac tttttattct ctggaaaacc aacttttgtc ctgcagtgtt gaaaaccgat
     1201 acagttgcat cagaagctac accaccattg cagttaaccg aaatctggtt gcgggagttt
     1261 ttatagattg ttgttgcctg ttcatcaacg agggtaccat taccggtatt gatgcctgtg
     1321 gtaacactcg tggttctgag tatcagactt aaattcttag tggaacctgt agaagcagta
     1381 aaagtataaa cagtgcttcc gtcagtcaga tttgcttcat ttccggtttc catatctttt
     1441 ataatcaagc taatacctga tgccagattg ttgatttctg ttgccttcag ggtaaatgag
     1501 gttgaagttg attgattagc agcaaaactg attggtattt cggtatccag aggaagtgca
     1561 ttcatcccat tgatagccag attttcacca tcaactgtag tatataaatc gggaactaat
     1621 gatgtggtag aattcatacc ttttgatgag tcataatcat cgtaaccgtt tgaagctgac
     1681 ccgtcggaat atattacagt ttcatctgca taagtaccgt ttgacacttg aagtcggata
     1741 agcagaaggt ttgaataatt cactgttggc gctctgaaaa cattgccggt cacatctctg
     1801 tgagtacgta gagcattagt aagtgagagt gtggaagctg tttttccttt ctttactctt
     1861 acccaaaatg cctgcattgg agctacgaca cctgttaccg gaagaccact gttacttgtg
     1921 tacacaccac ttttagtatt gtaaacatcg aacagggatg aagcatcctt acttttatac
     1981 cagattgtag tctcaatatc actgtttgtc agattattga gattgaggta ggacggatat
     2041 ggatttccca gcatattgaa acctgcattt agctgaccgg ctgtgcgggt cagaggtact
     2101 gagagattgc cattgtttat taaaccgttg aaaataacat taccattcgt aatagtcatg
     2161 tttgacagat atcctcttag aggcgttagt ttgccggtaa cattaaccca ttttgaagtt
     2221 ggctcgttat aagtcgtagt attggtagca gttgtaaatg tcgaagcctg ggctgacgta
     2281 acaggactac taatatggca attaagtcca cttgtcagat attgttgcac aacgtatgct
     2341 ccggttcctg taattgttgc aggagttaat aacgaagctg ttccttttgt attatccgaa
     2401 agcaagttta ggctgccatt gtttgtaagt gatgcaacca cagtcatctg gttgccgctt
     2461 cctattttga gcgtcgaacc agcattgatg atcaggctat ttactgtcaa tactgatgaa
     2521 gatgcagatc tgatttcaac agtcgtatta ttgagcaatt cgagttgatc aattgcttcg
     2581 gaaggcatat ttgttacaac aacactacct ccgttactga actgtaaaat atctttcgaa
     2641 gaaggacttg tacgggtagg cgtccagttt gaggcaactg cgtatgaagc tgttgtggtc
     2701 ttattccata catatgtagg agctgagttc atccttgccc gaccaataat ctgagcaggt
     2761 agaatagaag cagttactgt cgggtcataa acattggttg taggtccggc tccaagtatt
     2821 ggaagtacgt tgttatcaaa ccaatatgtt gatatttgtc cgtttctgtt tgtataattg
     2881 attttattgt tttcaatcgt caggtcatga cattcgtctc cggactgatt ccaggcataa
     2941 aggccaacat tagtaaatga ttgttgtttt ccgtatattt tattgtttcg tacagtaata
     3001 tgatgtccac ttgcaacagc tataccatat tgacccggat caaccagaat attgttttca
     3061 ataatataat acgaaccacc ataatcagca cacatgattc ctccacctga tggatcaggt
     3121 ccaccaccgc gtatccaatt atttgtaact tttatcggac tttcaggcgt tccgttagac
     3181 atatacatac ttaaacagtc gatcgtatta ctttgacccg gaatgttttc cataatatta
     3241 taacttatct catttccagc tccaaaacat tgattgagat atataatact gccatagttg
     3301 ggaagattat tgggattagc atttttcaat tcattattca tgaatttaat gtttccacga
     3361 catcctaaag cataaactcc agtggcaaca ctttcaaact cacagttggt aattgtgatg
     3421 tttgaacaat ccaccagatc tatagccaca taaccgggtg aatttgcaaa tctgcaattc
     3481 cgaattataa cattgttgca tgcttcaaga agaattgaac ttgttgtggc attgttaatc
     3541 tcaattcctt caataataat atcgttttta ctattatact ttaaaggaga ggatggtgta
     3601 taagaaccgg tgtaagttga tccctgacca taggtaagga acgggagcgt tgaaaatatc
     3661 agcataaata ataccgatat ttttttcatc agttgttttc ttttcatctt ttctaaaatc
     3721 attgtttgtt atctaattgt ttttattatc attcattaca gcacagagtc tcctctatca
     3781 aacttattgg atttaactaa cagcctcatt atgtgcattt atgcacataa gaaggttatt
     3841 ctatggttta ggaaattggt taaaaattaa ggtaggtata actcatgtaa tccgtgaaat
     3901 tggtttattt acttttcatt ctttctttga tgtcctaagc agattgttca ttcgtcaacg
     3961 ctaaaaactc aaacataact gatttattat cggctattta gtcattaact atgttaatgt
     4021 aagttttgat tgattattag taaatttctt aagacacttg tagaaaacaa ttctaattaa
     4081 atttactgct tcgatttgtc tgcaaaattt attatcaaaa acgacaaaca aacaaagttt
     4141 gaaacacaac tatttaaaaa cataaaaaca gcacaaaaat aatcagttac tacttgtgca
     4201 attttacaca attacgaaaa ttgtttatat atttttcagt tatacaatct tttaacgtct
     4261 taaacaaatt ataactgact aaatattgaa tctcatttat atttaaaatc atgttaataa
     4321 acaataaaca gacaaaagta ttttcttttt ctgttcagaa attacacaat tattgaaaat
     4381 aattacatag tttttagtta ttcaacaatt tatctgattt tggaagatat aacgaagtaa
     4441 aaaccgggta gaagacatga attttttttg gcttaaatgc ccgtcgaggg gagtgtgaaa
     4501 acaaaaaaaa ctaatatgtg gagaagagat ttatccgata tcccggatta tcatttttcg
     4561 ttgtttcatg tatttgaaaa acgaaggtgt caaacctgtc acttttttaa actgattgga
     4621 taaatgagct acactgctgt aattgagaag gtatgatatc tgggtcagat tcatttcatc
     4681 ataaatcagc agttctttaa ccctttcaat tctttggcta ataagatatt gttcaatggt
     4741 aactccggtc atttcggaga acagagtgga cagataagta taatcgtagt ttagtttttc
     4801 gctgaggtaa acggaaaaat ttgtttttaa ctgtacatca gtattttgaa tggtctcagt
     4861 aattacgttc tttattttct ggactaaatt gctttttctg tcatcgacca attcgagtcc
     4921 ggagttaact aaatttgttt tcagttttac aatctgatcg tagtcaacat cttctgttat
     4981 ctccagttct cctttctcca gctctccata gtgtaaaccg agttgatcga gttgttccag
     5041 catttctttt ttacactgga cgtaaagcgt gtattttatg tatagtttca tgctatcatt
     5101 cgaatgagaa atatgaataa tagatattat gacaagcaaa aaaaccagtt ttatctcaat
     5161 aaaatatcaa gttgaatttt taaaacctat actttttatc atcactgtta attcaatttt
     5221 catttttata ttcgtataaa atgaacttta gaagtatcac taattaatgc tttctcagtt
     5281 cactgatttc tttgtatttg caactcctga ttttcggcca attatcttgg ctggcagtat
     5341 cgatgacttt aattgcggat taaatatatt tgtctctgtc ccggcaacct tgcctacatt
     5401 ttcggcaaac cagagtattc ccctatcacc ggttttactg gtataattta tttcattgtg
     5461 tgcaattgtg agatcatgac attcagtttc agtctgattc catgcgtata gaccaacgtt
     5521 acaaaattcc tgtcgtcttg catatatctt attatatctc acagttatat gatgaccact
     5581 tgctaccgca ataccgtact ggcccggatc aaccagaatg ttgttttcaa ctaaaaaata
     5641 tgagcctccg caatctccca gcatgatacc tcctccccaa tcggcagggc ctccaccccg
     5701 aatccagtta ttgctgactt taatcgggct ctttgaggta ccgtttgatt tataaagact
     5761 gattatatcg ccgggaaaac tttcaccagc tatattttca caaatgttat agcaaatact
     5821 attcccaact ccacgcactt cattgaataa aaccatgcta ccataattgc gaagtttctt
     5881 cgaattaaca tttttcacct cattattttc aaatttaata tttcctgagc atccctgtgc
     5941 caataatgct gtagcaacat cttcaaatat acaattcgtt attttgacat ttgagcaatc
     6001 gcaaaggtaa atgcccacaa aagctgaaga aggtccgaac ttacaatttc gaattattac
     6061 gttatggcaa gcccaaaggg ttatgcaatg atttttgacg ctttttattt ctttaccatc
     6121 aatgataaaa ttcttcctgc cgttaatttc tattcgtgaa gatggagtat aagtaccctg
     6181 ataggtaact ccctgacata aagtaacgct tatgcttaga aacaatgcat ttattattgt
     6241 aagtattact tttttcatat ttcagtcgta tgataaatat agaataataa gaaattcaga
     6301 attcgattat ttgtaaatta ttgtataatt actcataacg tgacttaaaa tttatacata
     6361 taaaaacaaa taaattcctg tatttgttca atttatctgt ttcaaaaatg ctctttaaac
     6421 aattgaataa atcatatccc gaatatttct aacaggtaat gctttgatgt ttgcttttat
     6481 aaactttttc agtcatccat atgtttaatg caaaaagagt taatactaat tactttttac
     6541 taataacttt ttctttaatg tacacacatt gctcatagtt aacagatcaa caaaaactaa
     6601 ccaaacttaa ccaattatga aaaaagcttg ccacctatcg tatatctttt taatttctct
     6661 tttcattagt tcctgttctc aggttaagga cattgcctat tttcagaata ccgatgaaaa
     6721 agccattaca tcacaaatac aatcaagatc ggagagttta ttcgatccca taatcaaacc
     6781 aaaggatttg ctttccattt cagtagtgag caccgaacct ggtgcctcac gtatttataa
     6841 cctgattaca ccacaaacaa atggttcttc gggtgaaaat tctatcattt cacaacctgc
     6901 attacaaaat tatttggttg ataatgatgg gtttattaat ttcccggtat taggaaaaat
     6961 acaaattaaa ggcttgacta caaaagagtt gggagtaaaa atacaggata tgatagcacc
     7021 tgcattcact aaggagctac caattattac tgtgcggatt atcaattatt cagtaaatat
     7081 attgggcgaa gtaattaaac ccggaaaata tgaaactaca aatgagagac ttacgatatt
     7141 tgaaggtttg gctttagcgg gcgatatgac tatttatggt cgccgggaca atgttaaagt
     7201 actgcgtgaa gatgcttacg gaaatataaa atacatcaca ttaaatctga atgataagaa
     7261 tatcatcaac tcacccgctt attttctgga acagaatgat gtggtttatg ttgagcctaa
     7321 taaatcccgc tcagaatcct caaaattcgg ttctgccgaa aattttcgta tatcgacaat
     7381 atcagttctg ttttcagtgg tcaatatagc tgcaacttta tattcagcaa ttaaataatt
     7441 aaaaatcaca atagtatcat atctatattt ttcatatata aacgaaagat gttaaaatca
     7501 tgaataaata ccctgaaaaa gaagatgatt ataacccgtt gaccgaaaaa ccggtagact
     7561 ttatcgggct actaatcaaa tatatatctt actggaaatg gtttattctt tccctgatta
     7621 tctgcattgc cattgcggca atctatctca gatatacact tccaaaatat gaagtgaaaa
     7681 cttcggtatt gtttaaggac gattcgaaag gaagtggctc agtagaaatg aatgtattta
     7741 atgacatgag tatcatcact caaaaaaata atattgacaa tatacttgaa ttgcttaaaa
     7801 attcagtaat aattgagaaa actgttaccg aactgggtct ttacacgtcc tacacgagca
     7861 taaacactat tcctttaatt caaaaaacgg gtattaaccg ttattttccc aattttggaa
     7921 gataccgtga gaaggtactt tatggtgatg aatgtcctgt actgataagc ctccctgaca
     7981 gcacgctgaa gtatttcacc ggtcaggtcg tttttgaagt gttgatgcat ccttatggct
     8041 tgtatgaatt ttcgggcact tatgccgaaa agaaatttaa cataaaagct tctttttcag
     8101 ataaaaaagt aatgttacca tttggcgtcg taaacataaa acgtggcaat attaaacttt
     8161 ccaaagatat gctgatagaa gttgccattc atcaaccctc taatgttgcg aattattttt
     8221 tatcacaaat gaaaatggta ttagcctcta aaacgggttc agtcgtagat ctttccttca
     8281 tatgttcaaa tgaaaatctc ggaaaagact ttattcgtaa atttattgag gtatataatc
     8341 gggaagaaat gaacgaaaaa atagaaatgg ccaataaaac ctcacaatta attgaagaac
     8401 acctgaatgc gctcagtagt gagcttagca gtgttgagtc tcaggctgaa aaatacaaac
     8461 aatctcaggg aattaccgat cttgcttctc aatctgattt atttaacaac cagactgccg
     8521 atgtggaaca aaaaagactt gaaatagaaa ctcaactggc catcgtttca gatctgaata
     8581 actacattca aaacagcgct aacagagacc agcttatacc ttcaaattcg ggcataaaca
     8641 gtggcaatct taacaatctg gtgagtgatt acaaccgact tatacttgag cgaaaccggc
     8701 tctctcgtat tgcatcaggc agcaatcagg caatgattga cctaacgaat cagattgaga
     8761 caatgtataa cacggttcag tcaagtatgc aaaatgaaag aaaaaatcta caaattgcac
     8821 ggcgcgatct gctttcaaaa tacaatcaaa ctagtgcccg gattcgggct attccacggc
     8881 aggaacgtca gtataccgag ataaaacgcc aacaaggagt gagggaagag ttgtttttat
     8941 atttgttaca aaaaaaagaa gaaaaatata tgaatatgtc ttcggttgtt ccaagttcaa
     9001 aattaattga caacgttcac agccttggac ctgtttcgcc cagaaaaaac attattttgc
     9061 tttcttcttt actcatcggt ctgctcattc cagttattgg gattaaagtc agagatttgc
     9121 tacgttatca aattgagaat aaggaagaac tggaagaaat atctatagtg ccggtattag
     9181 gtgaaatacc aagaacggat caatcgggca aggtacttgt taaagaaaac aacaccgaca
     9241 tttttacaga aatggtcaga cttcttcggg caaatcttct gttcgtaatg gataatccgg
     9301 acaaaaaagt gataaatatg ctatcgagtg taagcggtga aggaaaaacc tttgtaacta
     9361 tcaatctggc aattagtctg gctctgctcg ataaaaaagt gttgataatc gagctggatg
     9421 tacgcaaacc aaggctggcc gaatatattg gcattgaaaa caagtcaggt attactttgt
     9481 tcctttcggg acagataaat caaaacgagc ttattaagcc aactgatatt cacccgaact
     9541 tatcaattat tacagccggc acaattcccc ctaatcctaa tgaattactt gcaaaaccag
     9601 cactggatat gcttattgat gatttgcgcc atcagttcga ttacattgtc attgacacag
     9661 cccccattgg ggtagtatca gacagcttcc tgctgaaccg tctgacagat gttacgttat
     9721 atgtggtaag ggctgaattt acaccaaaaa aagacattga agatgcctct ttattgtaca
     9781 aaaagcaaaa acttaaaaac atgtacttta tactcaatga tgtagatctc aataaaaaca
     9841 catatcgcta cggctacggg aagaaatacg gatatggtta cgtttacgga aagaataagc
     9901 acacttatgg ttacggtacg aaaactgaaa aagcataagt ttatcattaa tttttatttt
     9961 aaattaaaaa aaatcgatat gttacctctt aaaggagccc ggataattga tctccccaaa
    10021 acactagacc ggcggggaaa tctttctgtc atcgaggaaa taaaaaatat tccctttaaa
    10081 ataaaacgca catactggat ttacgacgta ccgggaggcg aaatcagagg cggacatgcc
    10141 tacaaggcta ataaggaatt tatagtagct ctgtcgggca gttttgatgt tatcctcgac
    10201 gatggcaagg aaagaaaggt attttcactc aaccgttcgt actatggact gtatgtacca
    10261 aaaggtgtat ggcggcaaat gcagaatttt tcgaccaatt cgctggcatt gatactggct
    10321 tctgccaaat ataatccgga tgattacatt tttgattatg agttatttaa aaactccatt
    10381 atatgaaaaa aacaacagtt tacgattgct ccatcattga aatagataaa catcatcacg
    10441 aaaagggaaa cctgagcgtc attgaaaacg gcaaaaccat tcctttcggc gtcaagcggg
    10501 tgtattatct gtacgatgta ccgggcggag agtcccgggg cggtcatgcg cataaggaac
    10561 tcaggcagct cattatagca gcaagtggca gctttgatgt taaactggat gacggcagtc
    10621 agaaaaggac ctacaccata aacagaccct atgaagggat acttgtcgtt ccgggaatat
    10681 ggcgcgaact cgataacttc tcttccggat cagtttgcct ggtgctggca tccaccgaat
    10741 acgaggcaga tgattatatc cgtgattata atgaattcat agcatataag aaaatatgat
    10801 tcatccactt tctgacgtgc ttagcactaa tattggtgag aacacgaaaa tatggcaatt
    10861 ttgcattgtt ctaccggatg ccgaaatcgg agaaaactgc aacatatgtg ctcatgtact
    10921 gattgaaaac aaggtgaaaa taggaaacaa cgtcactata aaatcgggag ttcagcttta
    10981 tgacggcgta accctcgagg actacgtatt catcgggccc aatgtaacat ttaccaacga
    11041 aaaagtgcct cgttccaaaa catatgacaa ctctaaaata aaaaatacac ttataaaaaa
    11101 gggagcttcc attggtggaa atgctactgt tattcccggc attacaattg gtgaatatgc
    11161 actcgttggc gccggaagtg tgattaccaa agatattgaa aaaaatacag tatggtacgg
    11221 aaatccggcc agaaagaaag gttacataac cgatgatggt attctgctgg atataaataa
    11281 aaaggacaaa gatggaaaat attatgaatt aaatttatag caatgataaa gtttcttgat
    11341 ctgcaaaaaa ttactcaaaa gtattctgtc gaaatacacg aggctgtttc cggagttatc
    11401 aactccggat ggtatcttac gggtgctgaa aatctgaaat ttgaaacaag ttacgcacaa
    11461 tttatcggta caaaatactg catcggagtg gctaacggcc tggatgcttt gaggctgatc
    11521 ttaagggcat acattgaaat gggcatcatg caagcaggtg atgaaatcat tgtacctgcc
    11581 aatacattca tagcatcaat acttgccatc acagataatg gtcttattcc ggttctggtt
    11641 gaacctgaca tcaacacata ccagacagac aacagtaaaa tagaagaggc tattaccccc
    11701 aaaacgaaag gtttgatgat cgttcacctc tacggccaat gtgcttatac agagaaaatt
    11761 gcaagtgttt gcaaacaaca taaccttaaa ctaatagaag acaacgcaca agctcatgga
    11821 tgcaaattca acggtataac taccggttcg cttggcgatg cagccggaca cagcttttat
    11881 cccggtaaaa atctgggagc attcggtgat gccggagcag ttaccaccag cgatgaaaaa
    11941 ctggcggaaa tggttcgcat cctcgctaat tacggctcat ctcataaata catttttgaa
    12001 tatcagggac tgaacagccg cctcgacgaa attcaggcag caatcctgag cgtaaagctg
    12061 aaacatctga atgaagataa cgagcgcaga aaagagatag cgaagtttta tatagaaaat
    12121 atcaaaaatc ccaccgtcat tacaccaaag gtaaatgatt ggaatgcaaa tgtatttcac
    12181 atatttccaa tcagatgcag gcgtagggat gacttacagc gatatctggc tgataataac
    12241 attcagacca ttattcatta tcccgttccg cctcacaaac aggcttgtta taaagagtgg
    12301 aaccagcttt cgctgcctgt aacagaacaa atacacaggg aagaattaag tctgccaata
    12361 agcccggtac ttaccgatga agaagcagaa atggtggtca gatttgtaaa ttcatttcgt
    12421 tgatgaaaaa aaatatatac atattcctgc gaaagtttgc aaagaaaaca ctcactttcc
    12481 cttattctgt ttttacaata ttagtttgta aaataagatc catttatttt tcaagtaaaa
    12541 tagacgatgg cgggggcaaa ataataattt cagatacttt cttggatttc aaaattaaca
    12601 aacatccaac tgccagatta attattaaag gtaatttgaa cacaataaag catgtcggaa
    12661 ataaaaatcc aatctatatc gaacttgggg aaaattcaac acttagtatc gaaggagact
    12721 ttacaatagg cagaggagtt agaattacgt tatatacgaa ctcggagtta ataataggcg
    12781 gcagagaaaa agaatccgat tcggggatta cagccgacac gttaatcatg gtttataaaa
    12841 aaatctcatt gggcaaagat tttatgtgtg cttggaatgt gtttatctcc gatagcgact
    12901 ggcatcacat cgaaggtcaa agtcatcatt cggatatcat tataggtgat catgtgtgga
    12961 ttgccaatag caataacata ttaaaaggct ctgtaattgg caacaataca attgtagcaa
    13021 gtaattcaaa aacaataaat tcggtttatc ccgacaatgt attaatcggt ggagtgccgg
    13081 caaaaatact gagagaaaaa attggatgga accgggatgt gtaatctatt taaaagaata
    13141 cttgattgaa aatgcacgaa caacaatctt catatcgaca aataatgaaa gccacttcgc
    13201 tattcggtgg agtacaggtc tttcaaatac ttatttcggt aatacgctcc aaatttgtag
    13261 cagtcttact gggacctgaa ggcatgggaa tagttggact tcttactacc actaccggat
    13321 ttattggagc tttaactaat ttcgggctaa gcacaagcgc agtcaaagac atatccgaat
    13381 caaatacatc gggagatcaa aaccgcatat cgggagttat ctccgtaatg cgtcgattag
    13441 cctggatcac gggtttgttg ggggcgattg ttacgttgat tttatcaccc tggctaagtc
    13501 agctcacttt tggaaataaa gattatacgc tggcatttat ctggatttcg gttactttac
    13561 ttttcaatca gctaagcagc gggcagcttg ttattcttca gggattacga aaattgcaat
    13621 acctggctaa agcgaactta tatggtagtt tagtcggttt ggttatcgtt gttccattat
    13681 attataagtt tggtattcgt ggaattgttc cggtgattat tgtaacatcc cttatttcgt
    13741 tatttttttc ctggtattat tcccgaaaag tagaaatcaa acccatgcag atatcaaaag
    13801 caaaaactat ttcggaagga aaaaacatgc ttacgatggg ttttgtaatc agccttagtg
    13861 gtttgatgac tatggcgaca gcctatctgc ttcgtatcta tataaaccga accggcaatg
    13921 tagctgatgt tggactttat agtgccggat tcaccatgat cggaacctat gtgggtatga
    13981 ttttcacagc tatggggact gattattatc cgagattatc tgcggtagca aaaagcaatg
    14041 aattaagcag acaggccatc aaccaacagg cagaaattgc aattttgatt atcgcaccat
    14101 tattaattgc attcctggta tttatcaact ggttcgttat tttactctat tccaatcaat
    14161 ttgttgccat taacggaatg ctgtattggg cctccctggg catgttcttt aaagctgcca
    14221 gttgggcaat tggatttatt tttctggcaa agtctgccag caaaatttat ttttggagcg
    14281 aacttgttgc cgaaacttat tcattaattt tcaatattat aggatatcat ttttggggat
    14341 taaacggttt agggttctca tttttagctg gttatgtaat agtttttgta caggtttaca
    14401 ttatagcaaa aattaattat caattttcat tcaccagatc attctcactc atttttacta
    14461 tcgagtttct gttagctatg ctgagtttta cggctgttaa gtttcttaat cagccatacc
    14521 cctatattgt cgggagcata ttaatagtac tgtctgcatg gtattcattt cgtgagttgg
    14581 agaaaagaat tggcataaaa gagttaattc aggggttttt gcaaaagaat atcaaaaact
    14641 aaggacatca tctatatgaa agaaccgaaa aagccattaa tttcaattgt tgttataaca
    14701 ttcaacagtt caaagtatgt gctcgaaacg ctcgaaagcg ccaaagcaca aacctatcag
    14761 aatatcgaac tgattgtgac agatgactgt tctgtggata atactttgtc tgtatgtacc
    14821 gaatggatta aaaaaaacaa aaaccgcttc accagaacag aaataatttc tgttgagaaa
    14881 aatacaggaa ttgtcccaaa cctaaacaga gggcttaatg cagttaaagg tgaatggatt
    14941 aaatttattg ccggtgatga tagactaaat gacaattgta tcgaaagatt ctatgaagag
    15001 gtctaccaaa attccgaaat agaagtcata ttttcaaatg tatctgttaa tggcattgat
    15061 ataccggaag attacttacc aaaattcttt ttgctggaaa caaaggaaca gtacctgaat
    15121 ttattaaaag ggaattcttt accggctcct gcttttttca tcaaatcaac aacgttgaag
    15181 caacttaacg gttttgacga acaatttcct tttctggatg attatccttt attcattaaa
    15241 atactaaaaa gcggtagaaa actctattat gttaatgatt ttctggttta ctatcgcgac
    15301 aacaatgaca gtgtatctca cagcaaagta atgaacatca actacgtaaa gagtttaaag
    15361 ttattctata aagaagtatt attaccagaa ttgacggcta acaagatgta tttttacgca
    15421 cttcattatc ggctggaata tttgttgtta ttcttttctg aacatgaatt aattaaatac
    15481 aaaaaagact atttccgatt gttaaatata ttcagcgcgt taatctggaa agctaaattg
    15541 ggaaaattac agagaaactt ttacaacaaa ctattactgt tcatttcata aggtataaat
    15601 aaaccgaaaa taatgctata atgtaaaata caacaccaaa tgttcaatat actatagagc
    15661 aaataaacaa gaatgctgaa gacctcaaac atattatttc tttctgacaa cgaagagcgc
    15721 accgatgaag aatcaactgt tgcttcacta aaaagttcgt atgacaccaa actgcgacgt
    15781 gtggtaaact atttaccctt attcttctta ttatgtttta gcggcaatcc gataatttca
    15841 tccatgggtt attctaaaat gttgcttgtt ctttattcgt ttgcttttat tgtatatgtc
    15901 ttcagctttg tgaattttga atatataaaa ttaaagataa atcagatatc accggtaatc
    15961 gtattcatag ttcttttagt gatatatcag cattttttat tgggaaaggt aagttatccc
    16021 ggggtaatag tattagtttt aaaaatattt ctgggtctgt tcactttcat tttttatcag
    16081 gcaaaacgcc tcagttttgt taaaaccttt ataaaaatca tggcattttt ggctataatc
    16141 agtttaccct tctttatcct taatcaattc atccaaattg gaatacaact cgataatata
    16201 tttcttaaat ctctaattct atacacctca taccctgccg aaactttttc agaaagttta
    16261 ctggtccgca atccgggcat gttttgggaa ccgggtgcat ttgcaggtta tcttattctg
    16321 gctctccttt ttgtcattct cgaaaaccgg agttttacga ttggtaatta taaaaaggaa
    16381 gtattcggca tacttctggg actaataact tcccagtcaa cgacaggata tttcttactc
    16441 ggaattttaa tactattatt tatatggaac agatatagtt ggggcaaaat cattatagtt
    16501 cctataactc ttttgctggt ttactggggc tacaataata tcgcatttat gggcaataaa
    16561 gttgaaaatc aattttccaa tgccatggct atgggagaaa gtgatgtttc taatacccgt
    16621 ttcggagctt tgaatatgga cttgatgtat atcaggtcac aacctcttac aggtaacgga
    16681 ctggatattt cgaccagata tcgttttcat ccgtgggtca aagaagatat cggtcacggg
    16741 aatggcatgt caaactttgc tgcaatatgg gggattcctt tctttctcta ttttctttat
    16801 tgcgtttatc agtttgctca tcattattcc cgaaaaaaat taattgctat cgtttttctc
    16861 ctgaccatcg tattagtact ccagggcgaa caatttttaa attatccgat gtttttgagt
    16921 ttcttttctt taccggtttt ttacaggcag ttctcagaaa gcagaaaagg ttatttactt
    16981 aaggaagacg taaaaacaga tctaaatata aatttatgaa agttctctgg tttgcaaata
    17041 cgccttgtgg agctaccgaa aaattaattc cggatcacta tctgggaggc tggctcaaat
    17101 cattggaaca gcaattggtt ctgaataatg atattgaact aacggtctgc ttttattggg
    17161 aagaagctat tgaaccattc cgatataaaa acacaatgta ttacccggtc tttcggccag
    17221 gaagaaattc gaaaacaggg cgtttaataa acaggatact tcgcagagat aatgacaaaa
    17281 aagaaatcgg tgagcttgta aaagtcgtaa atacggtccg acctgatatt atacatattc
    17341 atggaacaga gcataattac ggtcttattc tggcacatac cgatattccg gcagttattt
    17401 ccattcaggg attattatcg cctttcagcg aaatgtattt ttccggtgtt ccttactgta
    17461 cagccattcg tttcgaagga ataaaaacaa agttattgtt cagatcatat agcagaacaa
    17521 ttgccgaact gaaaaagaga gcaactaaag agcgtgaaat tctgtcgcat acacaatttg
    17581 tcataggtcg aaccgattgg gacaggcgca ttacaggatt actttcacct tcaagtcaat
    17641 attttatctg taacgaaatg cttcggcctg ctttttacca gtttacgtgg cataaatcac
    17701 tatttaacaa cccattacaa atcgttacca tcatgaatag cgatttatcc aagggtcttg
    17761 aaacagtcat aaaaagtgct gaaatcctat gtttgcagaa ttcagttaca ttcaaatgga
    17821 taattattgg tctgaaagaa accgatgaac ttccgatgat gctaaagcgt tggttaaata
    17881 ttgattatca atcactgaat atttcttttt atggcctgaa gagcgaaaca gaagttgtgg
    17941 aaatactggt aaattcggac atctactgtc aggtaagcca tcttgaaaac agctcgaaca
    18001 gtctttgtga ggctatgctt atcgggatgc cggtgatagc tagttttgca ggtggaacag
    18061 atacaatcat agaaaacaat aaagaaggaa ttctatttca gaatggcgat gcctattcac
    18121 tggcagcaac aataaccgag ctatcaaaga attttgaaaa agcggtaaaa tatgggacca
    18181 atgcgcgtaa tatcgcctta aaaaggcata atccgaatga aattgtcagt aaacaaatag
    18241 aaatttatca ctctgttatt cagacaaccg agaaaaaata atataaaaac catgtattac
    18301 ccccgcgtac tatttattgg aattcagcgc ataaacgaaa gagacagctt taacaacggg
    18361 ctccttttgc gaaacctgtt cagaaactgg ccgggggaaa atatggccca gatcttttgt
    18421 tacgatgaca acggagataa aggattcttt cagcactact acaggattgg aagagctgaa
    18481 agaacatgtg gctcactctt cttcagactt aaaacacatg aaattcccaa atccggattt
    18541 gatattatca aacctccatc agaaatcagg aaacatggtt taaccgaaaa aataaaattg
    18601 caggctaaac ggtatcttgt tgataccgga atagttgaat taattttccg gccaaaaatg
    18661 tcttctgaga tgaaaacatg gattgatgaa tttaatcctc aggttatttt tgtacagggt
    18721 tataatctgt attttgtgtg gctggcaatg ttattaaaaa aaatgaccgg tgccaaactt
    18781 gcatttctta caacggatga ctggccctcc tatctttatt ccggtctgta cgaagagccg
    18841 acactattcc ggtggctgat gcgaccgata gtaaaatcgg ccacacagcg attgatgaac
    18901 aatgtggata taccttttgc ttttaacagt atcatgtcgg aggaatataa atcgcgttac
    18961 aataaaaaat tcattaccat caaccacgct gatgaggcag aaagttttaa aaatgctgtt
    19021 ccgcaaagat accaccccga taatgtcgtt tcaattgtta ccaccggata tttcaataaa
    19081 tatcgctggc cactgctgct cgatgtggat gaatgctgca ggaaacttac tgaaaaagga
    19141 aacccgatac gtttactggt tatttcgtcg ggtatggaaa aagagggaat tgctgctctt
    19201 agaaatgcaa aattcacaga tatcatacct gatccgggca acgaattgct tcgcggatat
    19261 ctgaaagcgg ccgatataaa tttattgcca gaagcattcg acccccaagt ggctgctgct
    19321 atcagcctgt cggtttccac caaagcccat ttgtttatgt taagtcagca tccaattatt
    19381 gtgtacggac atccaaatac aggagttgta aaatacgcca aatctgaagg ctggggcagc
    19441 gtagtttcga aacgggatct ttcactcctg acgaatgtaa tagagaaatt aattatcaac
    19501 aaggatgaag caaaacgact aattagttgt tcaaaaaata cttttgaaaa atatcactca
    19561 ctgagcaaaa atcaaaaaaa acttctcgaa gtattgcaat gattaagaac aacattaagc
    19621 gtatgtaaaa aatgaaaaaa cgacttataa aaataatgaa aaagtcaaat ggaatgctct
    19681 atatcatggc tgcaatatat agtttacgct ccttcttatt gataaaaggt tgcaaaaata
    19741 attcaattca gcttcgggga gcatttctga aaaatacacg tatccggata gacggtacaa
    19801 acaacatagt aaaaattgcc catgaaaaca gactaaataa ttgcctgata tacataaaag
    19861 gaaataattg tgaaatcttc attgaaaaac attgcattct tgccaatata gagttatgga
    19921 ttgaggatga caatggaaaa ataaccatcg actacagaac cacagctgaa ggaggacaca
    19981 ttgccgctac agaaggtaaa agtatttaca tcggtaaaga ctgcatgttt tcacatgata
    20041 tagagatact taacggagac agccatgcta tttacatgct ggagaataaa cttagaatta
    20101 atgcctcaaa gtcggtttat ataggtaatc atgtctggct cggatcaagt gttaaagtac
    20161 tgaaaggttc aaccatagaa gatggaactg tagtttctgc cggagcaatt gtaacaggtc
    20221 atctggaatc aaactctatc tatgcaggca taccggctaa aaaaatcagg gaaaatatat
    20281 tttgggaaag ggaaagataa tggaaaagaa aaaactactc atgtttcatc ctgcattagc
    20341 tccttaccgt attgatgagt ttaatgcctt gaactgcttg tatgagctga agattgtatt
    20401 tctgtcagaa aatttatgga atcagaagtt caatcaaagc agattattgt cacaattaac
    20461 gttccggtat tcatatttat taaaagggat aggttttaaa gaaagaatat tcaggttcgg
    20521 tatgctccgt actatcagag aattcaagcc tgatattatc ttgggatatg aattttctcc
    20581 agttactcaa taccttataa tacttaaaag actgggcatt atttctcaga aaatagggtc
    20641 tataacagat gatagcattg atatttgctc acatatccaa tcgacattca gatcaaccgc
    20701 acgtaagatt gctttaaagc atttggactt tttagttgtg atgtcacaag aagtctcagc
    20761 atattatcaa aaagagtttc atctgaataa aaaccaactc attattgccc ctataattca
    20821 agaccctgaa agactaagaa aaaattcaaa aactctcgaa aaaacggctt ctgaatatct
    20881 gcaaagattt caactagcag agaaaaaaat attattgttt gtcggacggc ttgtacagga
    20941 aaaaggatta accatttttc tggagactat tcatccttta ctattggaaa ataatcaaat
    21001 tcttgttgta atggtcggtg aaggagatga attaaaaaag ctgcaatcta tgacagaaca
    21061 aaaacatctg aacgacagaa taatttttcc cggaagatat gaaggcgatg aactctatgc
    21121 ctggtattta tgtgcttcgg gtttcgtatt gcccagtact tatgaaccct ttggtgcagt
    21181 agtgaatgaa gcactgattt ttggccagaa ggtattatgc tcacaatatg ccggtgcttc
    21241 cagccttatc agagcagaca acggacttat ttacaatccc ctaaacaaag aggaatcgat
    21301 agaaaaaatt aagttattta ttaatgacat agaaacagtc agggatgtca gtttgtcaaa
    21361 aaaaccgtcc ctgattacca accatcaaaa agaatttatt gaggagtggg aaaaaaccgg
    21421 attactttaa gttgctaatg gctgaaaaga aaaatatatt gtttattttg cacctaccac
    21481 cgcctgtaca cggctcttca atggtgggta agtcaattat ggatagtgag ctcatcaact
    21541 catcctttga atgccgttat gttaatttgt tgatgtcacg cacaataaat gaaaccggaa
    21601 aaccgggatt aataaaagtt ttacgtttca tgcgggtatg gttcagcgtt ttgggtgaga
    21661 taatcagaaa gaaacccgat ctgtgttatc tggcattaac agcatctgga actgcttttt
    21721 ataaagactt tttattggtt ctattgctgc gtatctttcg tatcaaacac atttatcatc
    21781 ttcacaacaa aggagtgaaa caaaatcaga cttcgaaatt aaataatcag ttataccggt
    21841 atgtatttag caaagcagaa accatacttc tttctaaaca gttgtatagt gatgtagaat
    21901 cgtttgtgaa agaatcggga gtttattttt gtccgaatgg aattaaggat acagtaatgc
    21961 ctgctttgcc ccgatcctgt cccgaaaact ctgccattaa actgttattt ttatccaatc
    22021 tgatgaaatc gaaaggagtc tatttattat tggatgcctg ttctatactg atgcataaaa
    22081 accttgattt tgaatgtaat tttgttggcg gcgaaggaga tatcagtttt gataacttta
    22141 ataaatatct gatagataaa ggattaaacc aaaatgcaaa atatcttgga ctaaagtttg
    22201 gggccgaaaa gcacaaggcg tttagagaag ctgatatttt cgttcatccc acatcaaatg
    22261 attgttttcc gttggtttta cttgaagcca tgcaaaatca gttaccgatt atttcgacct
    22321 ttgaaggtgg catccctgat attgttgaaa acgataaaac cggcttcttg atacctgcaa
    22381 ataatgccgg agcattagct gaaaaactgg aaattttaat taagaatccc gaaatgcgac
    22441 aaaaaatggg aagcgccgga agattgaaat ttgaaaaaga gtttaccctg gagatttttg
    22501 aaaaaaatct gcatgaaatc ttatataaag tgagtgaatg aagtgagtga aaataaaata
    22561 tttcttatct aaaatataat aattatggtt acagaagtct caaaagaatt atacagacaa
    22621 catttccacc aggatccaca tccttatatt tccgaggaat ttattgattt agtaaaaaat
    22681 aagacagata aagtggtacg actgattgaa tccgattata atgtgagtat tggattactt
    22741 ggtggtatta aggacggaat tctatattca cctttctctg ctccttatgg aggttttcac
    22801 tttataaacg acgaggtttt atatgaagat atattccggt ttttaaccca tcttaaagat
    22861 tatgttatcg ctgaaaagct taagaatgtt cacatcacac tgcccccgga tatttattgt
    22921 agttcgatga acgctaaatt cgtaaattca tttgtaaggt tggggtatat aatgaaaaca
    22981 cctgaaattg taaactggat agacttaaaa tcattcgatg gcaaatgggg gaaaaaagct
    23041 atagataaaa atattaaaaa aacaatccag aataaacttt cattcaaccc tgtaagcgat
    23101 gagaaacaga aaaatgaggc ttatgaaata atttatgata atcgaacatt gcagggacgt
    23161 gagatataca tgaacctcac tgatttggag aatgtaaata atatcttccc cgtggattat
    23221 tttctggtaa aaaacgtcaa caatgagaat cttgcttcag caatcttcta tcgtggacac
    23281 aataaaataa ttcaagctgt attttggggt gatattgtac caagaataca agttggagta
    23341 atggattttt tggtaatgaa tttattcttt ttttataaaa atctgggtta cgaatatatt
    23401 tgtcttggca gatcaagtgc agaaggtatt cccaatgaag gtttgctgcg ttttaaagaa
    23461 gcacataatt gcacatcttc actgagatac tcttttacct ggggacctga aagagtataa
    23521 a
//
LOCUS       DAOPYB010000029        22662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2305_length_22662_cov_10.189941, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000029 DAOPYB010000000
VERSION     DAOPYB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22662
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2305_length_22662_cov_10.189941"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PKH58_03900"
     CDS             complement(<1..796)
                     /locus_tag="PKH58_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNX88202.1"
                     /translation="MQPISSFNDLTSHLAEANCRKKLAVANAIDSHTLEAVLMAVEAG
                     IVEAYLVGDVAAIESPELFEKPSILPFIHIVDKPEVQEATIEAVRMVKEGEADILMKG
                     LVNTDVILRAILDKEKGLLPPGNVLTYNAALEIPGYHKLLFFSDPAVIPYPTLEQRKA
                     MIKYAINTCKKFGFQHPKVALIHATEKPNPKIMFMQDYLDIMQMWRMGEFGDVIMDGP
                     LDIFLALDNERGSIKNVPTPVLGDADVLIFPDFAAANVFYKGVALFA"
     gene            1082..1501
                     /locus_tag="PKH58_03905"
     CDS             1082..1501
                     /locus_tag="PKH58_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX88203.1"
                     /translation="MKQMITAQWMGDMAFETELNGHKLVMDAAEEGGGHDAGPRPKIL
                     MLAALAGCTGMDVVLILRKMRVEVVKCNVSVEADVTEEHPKHYNRMHVIYEFTGKDLP
                     LEKLEKAVRMSEETYCGVGAVYRKAIEVTSEIRMIEG"
     gene            1837..2427
                     /gene="ruvA"
                     /locus_tag="PKH58_03910"
     CDS             1837..2427
                     /gene="ruvA"
                     /locus_tag="PKH58_03910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNX88204.1"
                     /translation="MIDYIKGAITELTPANVVLETGGVGYFINIPLTTYTALNGKTEA
                     QLFVFEAIREDAYVLFGFLTRDERQLFLLLISVSGIGANTARVIMSSYTASEIQQMIA
                     GGDVVSLNAIKGIGTKTAQRIIVDLKDKILKVGTGTEMKGSLFPASSPVRDETVAALV
                     MLGFAASASQKVVDKIIRDDPSLSVEKVLKLALKML"
     gene            2509..9942
                     /gene="sprA"
                     /locus_tag="PKH58_03915"
     CDS             2509..9942
                     /gene="sprA"
                     /locus_tag="PKH58_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNX88205.1"
                     /translation="MFFKKKIFPVIILLTALVGTAYGLAGSSGSYDFDAAPDMQQPKS
                     QADTITRFPVSNQRQETYEDLNKQYPMDLRNPENVNSVVEYDVKTGNYVLRTKVGDME
                     ISTPYMLTPAEYQKYSLQNDMMKYWREKNSATAKNNEDKFSVTDMKFSLGPADKIFGP
                     GGVQLKTQGSAELIFGFKSNFIDNPALTERARRSNIPNFDQKIQLNVNGKVGDKINFG
                     LNYNTEASFDFDQKLVKLSYKGKEDDIFQNIEAGNVSMQLNSSLITGSTALFGIKTDM
                     QFGKLKVSAIASQQESQSQSVSSKGGSQTIPFEVPIDQYDDNRHFFLSHFFRNNYETA
                     LSKLPFISSGVIINRVEVWITNKRGNFDQARNVLAFMDLAETDSIDNKHWQPTGSTKL
                     PGNKSNTLYDEVTAITGVRDVKQSNALLASRYGSLDINGGEDYEKIESARRLDPSEFT
                     FNQSLGYISLRSSLNPDEVLAVAYEYSYGGKIYQVGEFSTDGVTAPDALVLKLLKSTT
                     QSPQLKTWDLMMKNVYSIGAMQMQKEDFELNVMYQNDSVGTMLQYLTEGEIANKRLLT
                     VMNLDRLDKKNEINPDGNFDYVEDITAQSSSGRIIFPVLEPFGSHLTKKLNNAALSKK
                     YVYQELYDSTLVVAQEYSEKNKFLLVGRYKGSANGSEISLNAMNVPRGSVVVTAGGAT
                     LTENVDYTVDYTMGTVTILNQSILESNTNIDVKLENQSMFSMQRKSLTGTHLEYLFSK
                     DFSLGGTIMHLSEMPLTKKVNTGSEPIKNTIWGLNTAWRTESQWLTNMLDKLPFVNAT
                     QPSNITFNAEFAQLLPGHSDVISNAGLAYLDDFESTKTNIDIHYPYYWYLASTPGLFP
                     ESSLSDNVEYGKNRALMSWYFVDPVLNGESRQTPPNLRGNAELQSDHRTRQVRETELF
                     PNKDLDPTRSSLLSVLNLSYYPSQRGPYNLDVDGMNSDGTLLNPKDRWGGIMRRLETT
                     DFETANIEYIEFWMMDPFIADKSNEAGGDLYFNLGDISEDILKDGKKAFEHGLPINEG
                     DANTTSTVWGRIPTTQSTVTAFDNAAGARINQDVGLNGLSTRQEFDFPAYKDYVEKLT
                     KKLTPEAVSRMQNDPFSPLKDPAGDDYHFYRGTDYDEQKLNIVERYKYYNGTEGNSPA
                     TENTGETYSTSATLLPDMEDINSDNTLNEYEKYYQYKVALRPGTMKIGSNYITDILDA
                     NVTLKNGKTETVRWYQFKIPVREYDDKVGSIRNFKSIRFIRMFLTNFSRETHLRFGTL
                     DLVRGEWKTYTKPLNALNAATASSSKLDVLAVNIEENGRKNPVNYVLPPGITRETDPS
                     QPQLLQQNEQSMVMRVTDLSAGEARAVYKNTTYDMRKYKRLQMFVHGEAMVDAAAQAQ
                     NLKDYELSCFIRFGSDMVNNYYEYEIPLKLTKPGVYLTDKLADREEVWQPQNMFDFPF
                     TVLTDAKLKRNKARQQGQTGVSNTTPFVVFDENNQNNKITVVGNPTISEVENIMIGIR
                     NVSGNVKSGEVWVNEMRMSEFDENAGWAGIANLSVGLSDLGSVNVSGRAETSGFGGLE
                     STITNRSMDDTYQMNLSAALDLGRFLPEQAKLQIPAYFTYTTETVSPKYNPLDNDILL
                     KDALDNLQSKLEKDSLKNVSQSVVTTKSFNVTNAKINVKSKKPKFYDPANITFSISSN
                     ETDEHTPEIFQNLTKQQRAAANYSFSFNNEPWEPFKKSKGMKSPALKLIKEFNVYYLP
                     TSLSYNTSLNRAYSQILLRDLLTGVSPLSQSTSSGSISSSKDFMWNRQFDIQYNITRG
                     LKVGLQTAMNANIAEPQFTPEIGKEYYEQWRDTVWYNIKKMGTPYTYQQVFDASWKIP
                     IDKLPLLDWITSNAQYNSTYSWNRSATMEGGTDIGNVASSMGSMSLDGTLNFETLYNK
                     VKYLKDVNARSQASLRPQNFVSKTYRQTVNLKAGTPLTVNHALGSQRLLLTAKDKNGK
                     TVSLKYTVKNAGTIELNPTADAESVAITLVSQNPNSRSAGQQFTDFTTRLLMSVRRAS
                     VTYRQTSSLTMPGFRYQPGILGLQTVDGLSAPGWGYVFGYHDNNTLKNAADNGWLYMG
                     TDVINPANEALTSDLDVRASIEPIAGFKIDLTAKRQTASNTVIHYMYEGMPSTFNGNF
                     NITQIALATTFDKIGTADQNYSSKTFDKFLANRQIIADRLNGKYTGTRYPSTGFFNES
                     PDYANKEYDAANGTFGLNSPDVLIPAFLSAYTGRDPNAVSTNPFLSILEMLPNWSLNF
                     NGLSNIGWVKEKVRSVSINHAYTNRYTIGNYSSFSTWVPMDGDNSALGYIRDVQNNTP
                     IPSSPYDISTVSLNESFSPLIGVNVAMKNSMTATAKYNKQRNLSLNLTSTQLIEADAD
                     EFVVGVGYVVNDFDVVMKMKSSKRQTVKNDLKLNADFSYKDIKTLLRKVNENITQASS
                     GNKVVSIKFTADYVFSSKVNLQLFFDRQMTAPLVSSTFPVSSTNFGLSFKFLLAR"
     gene            10066..>11156
                     /locus_tag="PKH58_03920"
     CDS             10066..>11156
                     /locus_tag="PKH58_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNX88206.1"
                     /translation="MKTRILLLLSVLIGLNVFGQIQEPVKWTFEKKITGKNTADLIFR
                     AKIDDSWHVYGLNIPPDGPKPTVIVFETIQNAEKAGGIQAKSKLIEAYDPNFDMKLNW
                     YANSAVFVQKITFKDAAKVFVKGYVEYMCCDDKSCLPPTKEEFKFGTPSANAATSQPE
                     TPVQENLQNGTAPINADAAVTTNPDSVKTQPVVVPDSAKIADYWTPVIDELGNYGGSE
                     GGSGARSLWWIFFAGLVGGFLALFTPCVWPIIPMTVSFFLKRSNNKTRGRRDATLYGL
                     AIVIIYVTLGILITFIFGASALNSLSTNAVFNLLFFALLVVFAISFFGAFEITLPASW
                     STKMDAKADSTAGILSILFMAFTLVLVSFS"
     assembly_gap    11157..11256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11257..11961
                     /locus_tag="PKH58_03925"
     CDS             <11257..11961
                     /locus_tag="PKH58_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX88207.1"
                     /translation="VADLAYGWHLLDRETFLALWIVIFALLGIYLLGKIRFPHDSETK
                     HTSVFGTFLAIISLAFAVYMVPGLWGAPLKAVSAFAPPLYTQDFNLYNNEVHPQFKDY
                     ETGMAYAASQKKPVILDFTGFGCVNCRKMEASVWTDPQVKRLLDEDFVLISLYVDDKT
                     PLAQPYQVQENGRTRTISTIGDKWSYLQRHKFGANAQPFYIMLDNKGKPLNKSFAFDE
                     NPDHFIQWLERENIRK"
     gene            12143..12712
                     /locus_tag="PKH58_03930"
     CDS             12143..12712
                     /locus_tag="PKH58_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNX88208.1"
                     /translation="MKTRKHSAEDEKIKDEVPVTDTNQESATEETTENEAAKIAEDLE
                     QLNQKCEELNDKNLRLMAEFDNFRKRTLKEKSELIKNAGENIFVNILPLVDDFERAMK
                     AMETTDDIVAVKEGIDLIYAKFIGFLDTNGVKAIPTENEPFDVDKHEAVTMFPAPAED
                     MKGKIIDCMTKGYTLNDKVIRFAKVVVGE"
     gene            12767..13933
                     /gene="dnaJ"
                     /locus_tag="PKH58_03935"
     CDS             12767..13933
                     /gene="dnaJ"
                     /locus_tag="PKH58_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNX88209.1"
                     /translation="MSGKRDYYEILEVSKSASVDEIKKAYRKKAIQYHPDKNPGDKVS
                     EEKFKEAAEAYEVLSDPQKRQRYDQFGHAGVGSSAASGGGFGGGMSMDDIFSHFGDIF
                     GGHFGGFGGFGGFGGSQRSGGQRVRRGSDLRVKVKLNLAEIAAGVEKKIKVKKYVSCQ
                     HCNGSGAASGSGMSTCSTCNGSGRITRVQQTILGQMQTTSECPTCGGDGKIIRDKCAH
                     CNGEGIVREEEVISINIPAGVMEGMQLSVSSKGNAARRGGVNGDLLVVVEEEPHPELI
                     RDDNDLIYNLLLSVPVASLGGSVEVPTVEGKVKVNIAPGTQPGKVLRLRGKGLPSVNR
                     YGTGDLLVNIGVYIPENLNKEEKQTMENLARSNNFKPNANASKGFFSHFRNMFE"
     gene            13964..16687
                     /locus_tag="PKH58_03940"
     CDS             13964..16687
                     /locus_tag="PKH58_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88210.1"
                     /translation="MKTSPITDGIGDVFFIDFGLIDADYHNYSVFLELKCLIPNSMKV
                     RCLPFLIVLSLFFGLKLQAQDSTEVKRISSFMQKYIRFSDDYPQEKVYLHFDNTSYYL
                     GETMWFKAYVVRSDRQSLSILSRVLYVELINPEGYVIETKKLKIEKGQCNGSFNLTAS
                     NYPGYFEVRAYTRYMLNFFEDNYFSRVFPIFDKPETEGSYVTKITERPRNQRIPVKRP
                     DVKTKNKLMLMFYPEGGSLVCGLNSKVAFKATDQNGQNAVIMGDIYNSKNRKVAEINT
                     EYLGMGAFIIQPDSNVTRAKVIFNNDEYSFDLPQPLKEGCVMNINSSDSVTLKITIQK
                     TLHFASGQLGLSVTCRGRLYLVQNIDFGMQNAVSLNVPKKMIPSGVSQVTLFDTKGEV
                     LSERLVFVNHRSNLKIDMTANKASFQPFDKVEMNFRVSDKSDRPLPLTFSLAVRDNAS
                     SLSSPYADNILTNLLLSSDLRGYIENPGYYFESNTASRKLALELLLLTQGWRKYDWKT
                     MTGVTPFNPQHPLEKELAAEGKITSLIRKKNLKDVEIMMILLTDSFSQRGTAKTDIDG
                     RFGFEMKDFNGEGKLILRSKINDRQKETNIMLDRNFSPGLKPYGFNELNFTDYFKINQ
                     NSDNKTFSTIPNELLEDSVLRKLSMSEKIHVLEELVVTEKRKPVKVSVKYNVSGEMDK
                     IKDTGDWEPASVEGFLEKLNQYFSYKTDSNGSAFTYKGKHVYFVRDDSPDLVAADIAE
                     LMNKSTADMVATENTTSTTESNTSTNSTSENQAFVQMPRFDEIESINIIEDYGSILRL
                     FNGDPKYDPTTICVAVMHLKKKYQEEPAGIRNTTFMGYSYPKEFYSPYYGQGIVPDPK
                     DYRRTIYWNPDVKTDNQGQATVSFYNNSTCHSMNVSAETVTENGIIGEMNK"
     gene            complement(16768..17646)
                     /locus_tag="PKH58_03945"
     CDS             complement(16768..17646)
                     /locus_tag="PKH58_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNX88211.1"
                     /translation="MNFKTEDFLVKQGSKLKLKELDTSFSAGINKKKGRKKLIKNIEE
                     LADFQEKFWADNRYSLLIILQAPDAAGKDGAIRHVMSGLNPQSCRVHCYKVPSKEELD
                     HDYLWRHYKDLPERGMIEIFNRSHYENVLATKVNPEFLMAENIPGIDKPEKADKKFWN
                     ERYEQINNFEKHIYQNGTHILKFFLNLSKEEQKHRLLARLEEKKKNWKFSSADLSARA
                     QWDEYQKAYEEMLEKTSTDYAPWYVIPADKKFFARVAIGEIIVEKLKSLDLMYPAGET
                     AEELEKARILLLNEKD"
     gene            18059..19816
                     /locus_tag="PKH58_03950"
     CDS             18059..19816
                     /locus_tag="PKH58_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HNX88212.1"
                     /translation="MKEIKFSLVYRDMWQSSGKYVPRIDQLQKIAPVIIDMGCFARVE
                     TNGGASEQVNLLYGENPNPSVRTFTKPFNEVGIQTHMLDRGLNGIRMNPVPADVRQLM
                     YKVKKAQGVDITRIFCGLNDVRNIALSIKYAKAAGMIAQAAMCITYSEVHNVEYYVNM
                     AEQLIAEGADEICLKDMAGIGRPDMLGKIVKAIKTSHPEIPIQYHGHSGPGFSVASML
                     EVAKAGVDYLDVAMEPLSWGMVHPDVITIQAMLKDAGFKVPEINMKAYMEARRLTQSF
                     VDDFLGYFIDERNKYMTSLLIGCGLPGGMMGSLMADLKGVHAGINTYLKNNNKPELST
                     DEMLVMLFDEVGYIWPKLGNPPLVTPYSQYVKNIALMNVMAMIKGQPRWGMIDKNSWD
                     MILGKAGKLPGKLDPEIIELAKKNNYEFFEGNPQDNYPDELPKFIKEMEKLGWDRGQD
                     DEELFEFAMHEKQYRDFKSGMAKERFDKELEAAIKEKYAQSEVQIPDIRALKYPNAEP
                     VVSTVSGRLLWELDFNEVSVEPPHGKHFKQFDMICAIQTNYGVEPIGSFCNGRIVGVV
                     KNQGDLVRKGEALAYIEKD"
     gene            19997..21460
                     /locus_tag="PKH58_03955"
     CDS             19997..21460
                     /locus_tag="PKH58_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNX88213.1"
                     /translation="MIKSLFIRKSLAQIETQAEESSLRKHLNLTNIILLGIGCIIGAG
                     IFVLTGTAAALHAGPAIAISFIVSAFGCLLAGLCYAEFSSMIPVSGSAYTYGYATMGE
                     FIAWIIGWDLILEYLFGSATVAVGWSGYVTSFLSDIGLQLPASICQSPFIFDASGWHA
                     TGALINFPAVFIVVLMTTLLVIGIKESASFNNIIVLIKVTVILLFIGFGIAHINVDNW
                     TPFIPANTGNFSQFGWTGILTGAAVVFFAYIGFDAVSTTAQEAINPKRDIPIGILGSL
                     VVCTILYVAVSLVMTGIVNYKDLNVSAPIAVAIDAAGKSLRWLSPIIKIGAIAGLSSV
                     VMVLLLAQSRIFYTMASDGLLWKSFAKTHPKFKTPYITSIVTGCFAALFAGLFPIGLL
                     GELVSIGTLLAFVIVSVGLIILRKTEPDAHRAFRTPFVPLVPILGALVCLVQMASLPL
                     DTWIRLLGWMAIGFIIYFTYGIKHSRVRKEKQNNQSV"
     gene            21744..>22662
                     /locus_tag="PKH58_03960"
     CDS             21744..>22662
                     /locus_tag="PKH58_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNX88214.1"
                     /translation="MEENEKLAEEVNQTPEAVEETQQPAVEETAPVVEEAAPVAEEVI
                     EEAPAAKKETPKYVKPTGPAADFDWEAYEKGEVLTGKSKDELEKIYDDTLNAIKDKEV
                     VEGTVTSINKREVVVNVGFKSDGVVSMSEFRYNPDLKVGDKVEVYIESQEDKKGQLIL
                     SHKQARATRSWDRVNAALESQEVVKGFVKCRTKGGMIVDVFGIEAFLPGSQIDVKPIR
                     DYDIFVNKTMEFKVIKINQEFKNVVVSHKALIEEEIEQQKKEIIGKLQKGQVLEGTVK
                     NITSYGVFIDLGGVDGLIHITDLSWGRVSH"
BASE COUNT         6942 a         4601 c         4905 g         6114 t
ORIGIN      
        1 cggcaaacag tgcaactcct ttgtagaata cattggctgc tgcaaaatcg ggaaatatta
       61 gtacatcagc atctcccagc acaggtgtcg gtacattttt tatgctgcca cgttcgttgt
      121 cgagtgccag aaatatatcc agtggtccat ccattatgac atcaccaaat tcgcccatac
      181 gccacatttg cattatgtcc agataatcct gcataaacat gattttgggg tttggttttt
      241 cggtagcatg tatcaatgcc actttcggat gttgaaatcc aaattttttg caggtgttaa
      301 tggcgtattt tatcatagcc ttgcgttgct ccagcgtggg gtagggaatt actgcagggt
      361 cagagaaaaa tagcagttta tgatacccgg gaatttcgag agctgcgttg taggtaagca
      421 cattacccgg agggagcaat cctttttctt tatccaaaat agcccgcagt atcacatcgg
      481 tgttcaccag ccctttcatc agtatatcgg cctctccttc ctttaccatg cgtactgctt
      541 cgatggttgc ctcctgaact tcaggtttat ccacaatgtg aataaaggga agtatcgacg
      601 gtttctcgaa cagctcagga gactctatgg ctgcaacgtc gcccaccaga tatgcttcca
      661 caatgcctgc ttccacagcc atcagtacag cttcaagtgt atgtgaatca atagcattgg
      721 caacagccag ttttttccgg caattggctt ctgcaaggtg tgatgtcagg tcgttgaagg
      781 atgaaatggg ttgcatatga ttatgtttct acattcattt ctgcaaatgt attaatttat
      841 tttcagcctg caagtcctgc aaacggttgg aagggaagtc atttctggtc taaaattaca
      901 aatgtttatt caccaaattg ttttctttca aatttgtaat attttgctcg tttccattaa
      961 aattacatcg aaaaaacagg aaaaatattt tttgaaatat ttcatccgta tgcatgaaaa
     1021 aactttttta atccttaaat gtttatacat caaacaaaaa atcaaataaa aaaatataat
     1081 tatgaaacaa atgattacag ctcaatggat gggcgatatg gcattcgaaa cagaactgaa
     1141 cggtcacaaa ctcgtgatgg atgccgctga agagggcgga ggtcatgatg ccggtccgcg
     1201 tccgaaaata ctgatgctcg cagcactggc cggttgtacg gggatggatg tggttttaat
     1261 tcttcgcaaa atgagagtgg aagttgtgaa gtgtaatgtc agcgtggagg cagatgtaac
     1321 cgaagaacat ccgaaacatt acaaccggat gcatgtcatc tacgaattta ccggaaaaga
     1381 tcttccgctt gaaaaacttg agaaagccgt ccgtatgtcg gaggaaacct attgcggcgt
     1441 gggcgctgtt tatcgaaagg cgatagaggt aacttcggaa atcagaatga ttgaaggata
     1501 agcataagca tcgcatccaa atgaactttc ggcgcataaa cgaccgaaag tttttttctt
     1561 taacaggttt cgttccgatt tcaatccatt acgggatctt acgatgttta atcgtagcca
     1621 ccgattgtga ttgtcttttt tcaatcatta ttaaatcagt caaaactctc acctgagtac
     1681 ataatttctc atccggattt taacattcag gcgtgtacta tttatttcaa aagtccgtta
     1741 atgattgcgg tacgctgtta tcgcacgata aagaatataa gttttgagaa atcagcggga
     1801 aaagttatct ttgtaggtta aaaaacagat taattcatga tagattatat taagggtgca
     1861 ataaccgaac taactcctgc caatgtagtt ttggaaacag gcggagttgg ttatttcatt
     1921 aatattccgc tgacaacata cactgccctg aacggaaaaa ccgaagcgca gttgtttgtc
     1981 tttgaggcaa ttcgcgaaga cgcatacgtg cttttcggat ttctgacacg cgacgaacga
     2041 cagcttttcc tgctgttgat ttccgtttcg ggaattggtg caaacactgc gcgtgtgatc
     2101 atgtcgtcgt ataccgcttc cgaaatacag caaatgatag ccggaggcga tgtggtgtcg
     2161 ctcaatgcca tcaagggaat tggcacaaag acagctcaac gcatcatcgt tgacctgaag
     2221 gataagatac tgaaagtggg taccggtacc gaaatgaaag gcagtctgtt tcccgcaagc
     2281 agccccgttc gcgacgaaac tgtcgcggcg cttgttatgt tgggttttgc agcttcggct
     2341 tcgcaaaaag tggtggataa aatcatcagg gatgatcctt cactttcggt cgaaaaagtg
     2401 cttaaacttg ccctgaaaat gctttaattc aatttagcgg attgaagaag ctgttgtttc
     2461 ttgtgagaaa acgtcagttt aatatcatcg cttgtaaatc tgttgtatat gttttttaag
     2521 aaaaaaatat ttcctgtaat tatacttctt accgccctgg tcggaaccgc ctatggatta
     2581 gccggatcat cgggatctta tgattttgat gcagcccccg atatgcagca acccaagtca
     2641 caggccgata cgataacccg tttcccggtg agcaatcagc ggcaggaaac ctacgaagac
     2701 ctgaataagc agtatccgat ggatttgaga aatcccgaaa atgtaaattc ggtcgtggag
     2761 tatgatgtga aaacgggtaa ctatgtgctt cgcacaaagg ttggcgacat ggaaatatcg
     2821 accccataca tgctcacacc cgccgaatat cagaagtact cgctgcaaaa tgacatgatg
     2881 aaatactggc gtgaaaaaaa ctctgcaacc gccaagaaca atgaggataa attttcagtg
     2941 accgacatga aattcagtct cggaccggcc gacaaaattt tcggacctgg cggtgtgcaa
     3001 ctcaaaactc agggatcggc agaacttatt ttcggtttca aaagcaactt tatcgacaat
     3061 ccagcgctta ccgaacgcgc gcgacgttca aacatcccta atttcgacca gaaaatacag
     3121 ttgaatgtga acgggaaagt gggcgataaa atcaactttg gcctgaacta caacaccgaa
     3181 gcatcgttcg attttgacca gaaactggtg aaactatcat acaaaggtaa ggaagatgat
     3241 atctttcaga atatcgaagc cggtaatgtg agcatgcagc tcaacagttc gctcataacc
     3301 ggaagtacag cgctgttcgg tattaaaacc gatatgcagt tcggaaaact gaaagtgtca
     3361 gctatcgcgt ctcaacagga atcgcaatcg caatctgtca gttcaaaggg aggctcacag
     3421 actatccctt tcgaagttcc catcgatcag tatgacgaca acaggcattt tttcctttca
     3481 catttcttcc ggaataatta tgaaacggcg ctcagtaaac tgccgtttat ttcttccggt
     3541 gtgataatca accgcgtgga agtctggatt accaacaaac gcggtaactt tgatcaggcg
     3601 cgcaacgtac tggcatttat ggatttggcc gaaacagatt ccattgataa taaacactgg
     3661 caacctacag gaagcactaa actacccggc aataaatcaa atacacttta cgacgaagtg
     3721 acggctatta ccggagtacg cgatgtgaaa cagtcaaatg cgttgcttgc atcaaggtat
     3781 ggatctctcg acatcaacgg aggcgaagat tacgaaaaaa ttgaaagcgc gcgtcgcctc
     3841 gatccttccg aatttacatt taaccaatcg ctgggttaca tttcattacg ttcaagtttg
     3901 aaccccgatg aagtacttgc tgtggcatat gagtattctt atggcgggaa aatttatcag
     3961 gttggggaat tctctaccga cggagtgaca gcgcccgacg cgcttgtgct caaactgctg
     4021 aaaagtacta cacaatcacc ccagcttaaa acgtgggatc tgatgatgaa aaacgtttat
     4081 tcaatcggag ccatgcagat gcaaaaggag gattttgaac tgaatgtaat gtatcagaac
     4141 gactctgtgg gtaccatgct tcagtatctg accgaaggcg agatagccaa taaacgtttg
     4201 ctcactgtga tgaacctcga ccggctggac aaaaagaacg aaattaatcc ggatggaaat
     4261 ttcgactatg ttgaagatat tactgcgcaa agttcatccg gacgaataat tttccctgtt
     4321 ctggaacctt ttggcagcca tctgacaaag aaactgaaca acgctgcact cagtaaaaaa
     4381 tatgtgtatc aggaattgta cgactctacg cttgtagtgg ctcaggaata cagtgaaaaa
     4441 aataaatttc tgctcgtcgg acgttacaaa ggatcagcca acggttcgga aataagcctg
     4501 aacgccatga atgtaccgcg tggctcggtt gtagtaactg ccggaggcgc tacactgact
     4561 gaaaatgtgg attataccgt ggattatacc atgggaaccg ttacaatcct gaatcagtcg
     4621 attttagaat ccaatactaa tatcgatgta aaactggaaa accagtccat gttcagcatg
     4681 cagcgaaaat cgcttaccgg aacacatctg gaatatctct tcagcaaaga cttcagcctt
     4741 ggcggaacca tcatgcacct ttccgaaatg ccgctcacca aaaaggtaaa taccggaagc
     4801 gaacccatta aaaataccat ctggggactg aataccgcat ggcgaacaga atcccaatgg
     4861 ctaaccaata tgctcgacaa actgccgttt gtcaatgcca cacagccttc gaatattacc
     4921 tttaatgccg aatttgcaca gctgctgccg ggtcactccg acgttataag caatgccgga
     4981 cttgcttatc tggatgattt cgaatcgacc aaaacaaata tcgatataca ctatccctat
     5041 tactggtatc tggcaagtac accgggactt ttccccgaat catcgctgag cgataatgtg
     5101 gaatacggga aaaaccgtgc gctgatgtca tggtattttg tggatccggt actcaacggt
     5161 gaaagccgcc agacacctcc aaatctgcgc ggaaatgcag aactgcaatc cgatcaccgt
     5221 acccgtcagg tacgcgaaac tgaacttttc cccaacaaag acctggatcc aacccgttcc
     5281 tcgctgctat cggtgttaaa tctttcatat tatccttctc agcgcggtcc ttacaacctg
     5341 gatgtggacg gaatgaattc cgacggtaca cttctcaatc cgaaagaccg ctggggaggt
     5401 attatgcgtc gactggaaac taccgatttc gaaactgcca atattgaata cattgaattc
     5461 tggatgatgg atccgtttat tgcggataaa agcaacgaag ccggaggtga tttgtatttc
     5521 aatctcggtg atatcagcga agatattctc aaggacggaa agaaagcatt tgaacatgga
     5581 ctgccgatta atgaaggtga tgccaataca acatctaccg tttggggaag aatacctact
     5641 acacaatcta ctgtcactgc ttttgataat gctgccggag cacgtatcaa tcaggatgtg
     5701 ggtctgaacg gactttcgac ccgtcaggaa ttcgatttcc cggcttataa ggattatgtg
     5761 gaaaaactga caaagaaact tactcccgaa gctgtcagcc ggatgcagaa cgatccgttc
     5821 tctccgctca aagacccagc aggcgacgat tatcattttt accgtggtac cgactacgat
     5881 gagcagaaac taaatattgt tgaacggtat aaatactaca atggaaccga aggaaactct
     5941 ccggctaccg aaaatacagg tgaaacctat tcgacatcgg caaccctgct gcccgatatg
     6001 gaggatatca attcggacaa cacgctgaat gaatacgaaa aatattatca gtacaaagta
     6061 gctcttcgtc ccggaaccat gaaaatcggt tcaaattaca ttaccgatat tcttgatgcc
     6121 aatgttacgt tgaagaacgg gaaaacagaa acagtccgct ggtatcagtt caaaattccg
     6181 gttcgtgaat atgacgacaa agtgggtagt attcgtaact tcaaatcgat acggttcatc
     6241 cggatgtttt taaccaattt cagccgcgaa acacatttgc gtttcggaac actggatctg
     6301 gtgcgcggag aatggaaaac ctatacaaaa ccgctgaacg cactgaatgc ggcaaccgct
     6361 tcaagctcaa aactggatgt gctggcagta aatattgaag aaaacggaag gaaaaatccg
     6421 gtgaattatg tgcttcctcc gggcatcacg cgcgaaactg atcccagtca gccgcagctg
     6481 ctgcagcaaa acgaacaatc aatggtgatg cgggtaaccg atctgtctgc cggcgaagca
     6541 cgtgctgtat ataaaaatac cacttacgac atgcgtaagt acaaacgact tcaaatgttc
     6601 gtacatggtg aagcgatggt cgatgctgcc gctcaggcac aaaacctgaa ggattatgaa
     6661 ctttcttgct tcattcgttt tggctccgac atggttaaca actactacga atatgaaatt
     6721 ccgttgaaac tgactaaacc gggtgtatat ctgacagaca aactggcaga ccgcgaagag
     6781 gtttggcaac ctcagaatat gttcgatttt ccgttcactg ttctgaccga tgcaaaactg
     6841 aaacgtaaca aggcacgtca acagggacaa acgggtgtgt caaacacaac tccttttgtt
     6901 gtatttgatg agaataatca aaacaataaa ataaccgtgg tgggtaatcc gaccatttcg
     6961 gaagtggaaa acatcatgat tggtatccgc aatgtaagcg gtaatgtaaa atcgggagaa
     7021 gtctgggtca atgaaatgcg tatgtcggag tttgacgaaa atgcgggttg ggccgggata
     7081 gcaaacttgt cggtaggttt atcggatctc ggttcggtta atgtttcggg acgagctgaa
     7141 acatcgggtt ttggtggttt ggaaagtacg ataaccaacc gaagcatgga tgatacctat
     7201 cagatgaatc tgtcggcagc tctcgatctc ggacgcttcc tgcccgagca ggcaaaactg
     7261 cagataccgg catatttcac ttacaccacc gaaacagtat ctccgaaata caatccgctg
     7321 gataacgata tcttactgaa agatgcgctc gataatctgc agagtaaact ggaaaaagat
     7381 tcactgaaaa atgtttcaca aagtgtcgtg actacgaaaa gctttaatgt gacaaatgca
     7441 aaaatcaatg tgaaaagcaa gaaaccaaag ttctatgatc cggcaaatat cacgttcagc
     7501 atatcgtcca acgaaacgga tgaacatact cctgagatat ttcagaatct aaccaagcag
     7561 cagcgtgcag ctgcgaatta cagtttctcc ttcaataacg aaccttggga accgttcaag
     7621 aaatcgaagg ggatgaaaag tccggcactg aaactgatca aggagttcaa tgtgtattac
     7681 ctgcccacat cgttaagtta caataccagt ctcaacaggg catactctca gattttgctt
     7741 cgcgatttgt taacaggtgt atcaccgtta tctcagtcaa catcgtcggg cagcatatcg
     7801 tccagcaaag attttatgtg gaacaggcag tttgatatac aatataatat cacccgcgga
     7861 ctgaaggtgg gattacaaac agccatgaat gccaatatcg cagaacctca gtttacaccc
     7921 gaaataggta aagaatacta tgaacagtgg agagatacgg tttggtacaa catcaaaaaa
     7981 atgggaactc catacaccta tcagcaggtt tttgatgcat cgtggaaaat accaatcgac
     8041 aagcttccgt tactggactg gattacttcc aatgcacagt acaattccac gtatagctgg
     8101 aatcgcagcg caaccatgga agggggtacc gacattggaa atgtagcttc ttcgatgggt
     8161 tcaatgagtt tggacggaac tctgaacttc gaaactcttt acaataaagt aaaatacctc
     8221 aaagatgtta atgcgcgctc ccaggcatca ctaaggcctc agaactttgt gtcgaaaaca
     8281 taccggcaaa cggtaaatct gaaagctgga acgcctctga cggtaaatca tgcacttggc
     8341 agccagcggc tattacttac agcaaaagat aaaaacggga aaaccgtcag tctgaaatat
     8401 acggtaaaaa atgccggaac tatcgaattg aacccaacag ctgatgccga aagtgtagcg
     8461 attactctgg tgagccagaa tccgaattcc cgctctgccg ggcagcaatt caccgacttt
     8521 acaacccgac ttctaatgtc tgtcagacgt gcttcggtta cttaccgtca gaccagtagc
     8581 cttaccatgc ccggtttcag atatcagccc ggcatcctgg gtttacaaac tgtcgatgga
     8641 ctatcggctc cgggttgggg atatgtattt ggatatcacg acaataatac actgaaaaat
     8701 gcggccgata atggctggct ttatatggga acggatgtga tcaatccggc taatgaagcg
     8761 cttacttctg acctggatgt tcgtgcaagc attgaaccga tagcaggttt caaaattgac
     8821 cttacagcca aacgccaaac agcttcaaac actgtgattc attatatgta cgagggtatg
     8881 ccttcaacat tcaacggcaa cttcaacatc acccagattg cactggcaac aacttttgat
     8941 aagatcggaa ccgcagatca gaattattca tctaaaacat ttgataagtt tctggccaac
     9001 aggcagatca tcgctgacag gcttaacgga aaatacacag gtactcgtta tccgtcaaca
     9061 ggtttcttca atgaaagtcc tgattacgcc aacaaggaat acgatgctgc aaacggaacc
     9121 tttggcctca attcgcccga tgtactcatt cccgcgtttc tgtcggcata taccggacgt
     9181 gacccgaatg ctgtttcgac aaatccgttt ttgtcaattc tggagatgct tcctaactgg
     9241 agcctgaatt tcaacggttt gtcaaacatt ggctgggtaa aggaaaaagt tcgttcagta
     9301 agtataaatc atgcttatac caatcgttat accatcggta actattcctc attctcaacc
     9361 tgggtgccaa tggatggaga caacagtgcg ctgggatata ttcgggatgt tcagaataat
     9421 acaccgatac cttcttcgcc ttatgatatt tcaaccgttt cgctcaacga aagtttcagt
     9481 ccgctaatcg gtgtgaatgt agcgatgaaa aacagcatga cggcaacagc aaaatataat
     9541 aaacaacgta acctgtcgct caatcttacc agtactcagc tgattgaagc agatgccgat
     9601 gaatttgtag tgggagtggg gtatgtagtc aatgattttg atgtggttat gaaaatgaag
     9661 tcgagtaaac gtcagacagt gaaaaatgac ctgaaactca acgccgattt ttcatacaag
     9721 gatatcaaga cgcttctgcg gaaagtgaat gaaaatatca cgcaggcatc gagtgggaat
     9781 aaggtggtat caatcaagtt tactgccgat tatgtattta gttccaaggt aaatttgcag
     9841 ttgtttttcg atcgtcagat gactgcaccg ctggtgtcgt caacattccc ggtttcgtcc
     9901 accaatttcg gtctgagttt caaattctta ctggcacgat gagccgaaaa acctatagaa
     9961 ctattttgaa atatatataa atattcatga aaagataata gataatcaaa ccaaataaca
    10021 aattaatcgt tggttatctt atatctatta taattaatat tttgcatgaa aacaagaatt
    10081 ctcttactct tgtcggtttt aattggtctg aacgtatttg gtcagataca ggaaccggtc
    10141 aaatggactt ttgaaaaaaa aataaccgga aaaaatactg ctgatctgat atttcgggca
    10201 aagattgatg atagctggca tgtttatgga ctgaacatac ctcccgatgg tccaaaacct
    10261 accgttatag tctttgaaac cattcagaat gctgagaaag cgggcggtat tcaggccaag
    10321 tcaaaattga tagaagctta tgatccgaac tttgacatga agcttaactg gtatgccaac
    10381 agcgcggttt ttgtgcagaa aataactttc aaggatgcag caaaagtatt tgtgaaaggt
    10441 tatgtggagt atatgtgttg cgatgataaa agctgtctgc cgccaaccaa agaagaattt
    10501 aaattcggga cgccatcggc aaatgctgca acatcgcaac ccgaaactcc tgttcaggaa
    10561 aacctgcaaa acggaacggc gccaataaat gctgatgcag cagttacaac aaatccggat
    10621 tctgtaaaaa ctcagcccgt tgttgtacct gattcggcaa aaatagctga ctactggaca
    10681 ccagtcattg atgaactcgg aaattacgga ggttctgaag gaggaagcgg agctcgttct
    10741 ctgtggtgga ttttcttcgc cggactggtt ggaggatttc tggcactgtt tacaccctgt
    10801 gtctggccaa taatccccat gacggtaagt ttcttcctga aacgttcaaa caataaaacc
    10861 cgcggacggc gcgatgcaac actttacgga cttgctattg taattatata tgtcacgttg
    10921 ggaattctca tcacatttat ttttggtgca agcgcgctca acagcctttc gaccaatgct
    10981 gtttttaatc ttttgttttt cgcgttgctg gttgtttttg ccatttcatt ttttggcgct
    11041 ttcgaaatta cccttcctgc atcgtggtca acaaaaatgg atgcaaaggc cgattcgacg
    11101 gcaggaattt taagcatcct tttcatggct ttcaccctgg tactggtgtc tttctcnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtag ctgatctggc atacggttgg
    11281 catttactcg atcgtgaaac ctttttagct ttgtggattg tgatatttgc attgcttggt
    11341 atttatttac tgggaaaaat acgatttccg cacgatagtg agacaaaaca cacctcagtc
    11401 ttcggaacat ttcttgcgat aatttcactg gcttttgccg tgtacatggt tccgggtttg
    11461 tggggtgcac cactgaaagc tgtgagcgcc tttgccccac cgctttatac acaggatttt
    11521 aatctttaca acaatgaagt acaccctcag ttcaaagatt acgaaaccgg gatggcatat
    11581 gccgcttctc agaaaaaacc ggtgatttta gactttacag gttttggatg tgttaattgc
    11641 cgtaaaatgg aagcatcggt atggaccgac ccgcaagtaa aacggttgct ggatgaagat
    11701 tttgtactga tatctcttta tgtggacgac aaaacaccac ttgctcaacc gtatcaggta
    11761 caggaaaatg gacgaacccg aactatcagt accatcggcg ataaatggag ttatctgcaa
    11821 cgacacaagt ttggcgccaa tgcacaaccc ttctatatca tgcttgacaa caaaggtaaa
    11881 cctcttaata aatcctttgc attcgatgaa aatcccgatc atttcataca gtggctcgaa
    11941 cgggaaaata ttagaaaata acatttgctg tcaattttgt cagttagaat ataaattttc
    12001 ctgccaaaat gtcccgattt ctgccaaaaa tcactttcgg acattctggc aggttttttg
    12061 taagatgaat tatggcttat tttttatgcc ccgatgaaaa gattattaca ttaccataaa
    12121 agaataaaat tttaaaaacc ggatgaaaac aagaaaacat tctgcagaag acgaaaagat
    12181 aaaagatgaa gttcctgtaa cggatacaaa tcaggaatca gcaacagaag aaaccacaga
    12241 aaatgaagct gcaaaaattg ctgaagacct cgagcagcta aatcagaaat gtgaagagtt
    12301 gaatgacaaa aaccttcgtc tgatggctga gtttgacaat tttcgtaaac gtacgctcaa
    12361 agaaaaatca gaactgataa aaaatgccgg agaaaatatc ttcgtaaaca ttttgccgtt
    12421 ggtcgatgat tttgaaaggg caatgaaagc gatggaaacc acggatgata ttgtcgctgt
    12481 taaagaagga attgatttga tatatgctaa atttatcggt ttcctggata caaacggtgt
    12541 aaaagcaata cccactgaga atgaaccttt tgatgtggat aagcatgaag ctgtaaccat
    12601 gtttccggct ccggctgaag atatgaaggg aaaaatcatt gattgtatga ctaaaggata
    12661 tactttgaat gataaagtaa tccgttttgc caaagtagtt gtaggtgaat aaaataaaac
    12721 aattcaaaga ctggtggaag cttttctttg ataataagat ataaatatgt ccggaaaaag
    12781 agattattac gaaatattag aagtttcgaa atcagccagt gttgacgaaa tcaagaaagc
    12841 ataccgcaaa aaggcaattc aataccatcc cgacaaaaat ccgggtgata aagtctccga
    12901 agaaaaattc aaagaagctg ctgaagctta tgaggtattg agtgatccgc aaaaacggca
    12961 gcgttatgat cagttcggac atgcaggcgt aggaagcagt gctgcaagcg gtggcgggtt
    13021 tggaggagga atgagcatgg atgatatctt ctctcatttc ggcgatattt tcggcggaca
    13081 ctttggtggt ttcggaggtt tcggaggttt tggcggctca caacgcagcg gaggtcaacg
    13141 cgttcggcgc ggatccgatt tgcgtgtaaa agtgaaactc aacctggccg agattgctgc
    13201 cggagtcgaa aagaaaatta aggtaaagaa atacgtcagt tgtcagcact gcaacggttc
    13261 gggagcagct tccggttcag gtatgtcaac ctgctcaacc tgtaatggta gtggccggat
    13321 aacccgtgta cagcaaacca tactcggtca gatgcaaacc acttccgaat gtcctacatg
    13381 tggtggtgat ggaaaaatta tccgcgacaa atgtgctcac tgcaacggcg aaggtattgt
    13441 tcgtgaagaa gaggtaattt ctatcaatat cccggcagga gtgatggaag gcatgcaact
    13501 atcggtgagc agtaaaggaa atgcagcccg acgtggcggg gttaacggtg acctgcttgt
    13561 cgtggtggaa gaagaaccgc atcctgaact gataagagat gataacgacc tgatatataa
    13621 tttgttgctt tctgttccgg tggcaagtct gggtggatcg gtagaggtac caaccgttga
    13681 agggaaagtg aaggttaaca ttgctcccgg aacacaaccc ggaaaagtat tgagactgag
    13741 aggtaaggga ttgcccagtg taaatcgtta cggaacgggc gatttgctgg tgaatatcgg
    13801 ggtttatatt cccgaaaatc tcaacaagga agaaaaacag accatggaaa atctggccag
    13861 gtccaataac tttaagccaa atgccaatgc atcaaaaggt tttttctcac acttcagaaa
    13921 tatgtttgaa taggaaacaa aaactccaaa aatgctcata aaaatgaaga catccccgat
    13981 tactgatgga atcggggatg tcttttttat agattttggc ttaatcgatg cagactacca
    14041 taattattct gtatttttgg aacttaaatg cttaatacca aattcgatga aagtcagatg
    14101 tttaccgttt ctcattgtgt tatcgctgtt ttttggatta aaacttcagg cacaggattc
    14161 tacagaagtg aaaagaataa gttcattcat gcagaaatac atccggtttt cagatgatta
    14221 tccacaggaa aaagtatatc tgcatttcga taatacttcc tactatctgg gcgaaactat
    14281 gtggttcaaa gcgtatgttg ttcgttccga ccgccagtcg ctaagcatcc tgagccgggt
    14341 actgtatgtg gaattgatca accccgaagg atatgtgatt gaaaccaaaa aactaaaaat
    14401 agaaaaagga caatgcaacg gaagtttcaa tctgacagct tccaattatc ccggttattt
    14461 cgaagtcaga gcctacacgc gttatatgct taattttttc gaagacaact atttttcaag
    14521 agtattccca attttcgata aacccgaaac cgaaggcagt tacgttacta aaatcacaga
    14581 gcgacccaga aatcagagaa tacctgtcaa acgtccggat gtaaagacta aaaacaaatt
    14641 gatgcttatg ttttatccag aaggaggcag tctggtttgc gggttgaact caaaagttgc
    14701 cttcaaagca acagaccaaa acgggcaaaa cgcagtaatt atgggtgaca tttacaacag
    14761 taaaaaccgg aaagtagctg agataaatac cgaatacctc ggcatgggcg catttatcat
    14821 ccagccagac agtaatgtaa ccagagccaa agttattttt aataacgacg aatactcttt
    14881 tgatttgcct cagcccctta aagaaggttg tgtcatgaat atcaactcat cagattctgt
    14941 aacactgaaa ataactatac agaaaactct acatttcgct tccgggcaac tagggttgtc
    15001 cgtcacatgc cgaggcagac tttatctggt ccagaatatt gattttggca tgcaaaatgc
    15061 agtcagtctg aatgttccga aaaaaatgat accatcaggt gtgtcacagg ttaccctttt
    15121 cgatacgaaa ggcgaagtgc tgagtgaaag acttgttttt gtcaatcacc ggagtaacct
    15181 gaaaattgat atgacggcca ataaagccag ctttcagcca ttcgataagg tagagatgaa
    15241 tttcagggtg agcgataaat cggatcgtcc gcttccgctg acattttcac tggcggtaag
    15301 agacaatgcc agcagtctga gttcgccata tgccgataat atcctgacaa acctgctctt
    15361 gtcttccgat ttacggggat acattgaaaa tcccgggtat tatttcgaat caaatactgc
    15421 aagtcgaaaa ctggcccttg aacttttact gctcacgcag ggatggcgca agtatgactg
    15481 gaaaacaatg accggcgtaa caccattcaa tccgcaacat ccgctcgaaa aagaactggc
    15541 tgccgaaggt aaaataacct cccttattcg aaaaaagaat ttgaaagatg tggaaataat
    15601 gatgatactg ctgactgaca gcttttcgca acggggaaca gccaaaacag acattgatgg
    15661 ccgttttgga tttgaaatga aggattttaa cggcgagggt aaattgatat tgcgctcgaa
    15721 aattaacgat aggcagaaag aaaccaatat catgctcgac cgtaatttca gtccgggtct
    15781 gaaaccctac ggtttcaatg agctgaattt tacagattat ttcaaaataa atcaaaacag
    15841 tgataataaa acgttcagta ccataccaaa tgaacttctg gaggatagcg tactgaggaa
    15901 actttccatg tctgagaaaa ttcacgtgct ggaagagttg gtggttactg aaaaacgaaa
    15961 accggtcaaa gtcagcgtga aatataatgt gtccggtgaa atggataaaa taaaggatac
    16021 tggtgactgg gaaccggcga gtgtcgaagg atttttggaa aagctaaatc aatatttttc
    16081 atacaaaacc gacagtaacg gttctgcatt cacctataaa ggcaaacacg tttattttgt
    16141 cagagacgat tcacccgatt tggtagctgc tgatattgcc gaattgatga acaagtcgac
    16201 agccgatatg gttgcaaccg aaaacacaac ttcaacgact gaaagcaata cttccaccaa
    16261 ctccacaagt gaaaaccagg catttgtaca gatgccgcga tttgacgaaa tagagtcgat
    16321 caatatcatc gaagattatg gcagtattct cagactcttt aacggagatc ccaaatacga
    16381 ccctactaca atctgtgttg ctgtgatgca tctcaagaaa aaataccagg aagaaccagc
    16441 cggaatacgt aacacaacat tcatggggta ttcatatccc aaagaattct acagcccgta
    16501 ttatggacag ggtattgttc ctgatccgaa agactatcgg cgaacgattt actggaatcc
    16561 cgatgtgaaa accgacaatc agggtcaggc aacggtaagc ttttataaca acagtacgtg
    16621 ccatagcatg aatgtcagtg ccgaaactgt tacagaaaat gggataatag gtgagatgaa
    16681 taagtaagaa tgtgaattca aaaaaaaaag tctccggaga tttcttccgg agactttttt
    16741 tatatagaac ttctactaat acctagttca gtctttttcg ttaagtaata atattcgggc
    16801 tttctccagt tcctcagctg tttcgccagc aggatacatg agatcaagac ttttaagctt
    16861 ttcaacaatg atttcgccga ttgcgacccg cgcaaagaat tttttatcag ccggaataac
    16921 gtaccacgga gcataatcgg tagaagtttt ttccagcatt tcttcataag ccttctgata
    16981 ttcgtcccac tgggcacgtg ccgacagatc agccgaactg aatttccagt tttttttctt
    17041 ttcttccaaa cgcgccagaa gcctgtgttt ttgttcctct ttcgacaggt tcagaaaaaa
    17101 cttcaggata tgagtcccgt tttgatatat atgtttttca aagttgttga tttgttcgta
    17161 gcgttcattc cagaattttt tgtcagcttt ttcaggttta tctatgcctg gtatattttc
    17221 agccattaaa aattcaggat ttacctttgt ggccaataca ttttcgtagt gcgaacgatt
    17281 aaagatctca atcatcccgc gttcgggcag atctttgtaa tgacgccaca aataatcgtg
    17341 atccagctct tctttcgacg gaactttgta acaatgcacc cggcagcttt gcgggtttaa
    17401 cccggacatt acatggcgta tggcaccatc tttaccggca gcatcgggag cctgaagtat
    17461 tataagcaac gaatagcggt tgtctgccca gaatttttcc tgaaaatcgg ccagttcttc
    17521 aatattttta atcagtttct tccttccctt tttcttgtta atacctgctg aaaagcttgt
    17581 gtccagttct ttaagtttca gtttgcttcc ctgcttaact aaaaagtctt ctgttttgaa
    17641 gttcattatt atcaattatt aaaggttttg ctaataaata tattttaagt aaacaaatac
    17701 aaatataaga aagttcaatt gaaaaaaagt gtcattgtct gaaattttta ccttgttttt
    17761 ctgaaaatca gaattttttt tttattcatt ttcgaccagt ttaacatgat atgtgttatt
    17821 ctttgagtgc tttttttgta aaataaatat ttttataata ttaatgtttc agaatgtaaa
    17881 tacggaatat aaaaagcggg tctgattaag gaaaaatttc tctaatttta tttttttatt
    17941 cggaaaaaac ttcagaaatt tgaacaaaat tagaaactga atgatattgc attctatgct
    18001 atcagaatga ggtaataata aaaaaatctt ctatattcat caaacttatt gcttatctat
    18061 gaaagaaatt aaatttagct tggtctatcg ggatatgtgg cagtcgagtg gaaaatatgt
    18121 tcccagaatt gaccaactgc aaaaaattgc cccggtaatc atcgatatgg gttgctttgc
    18181 ccgtgtagaa actaacggag gtgcttccga acaggttaat ctgctatacg gtgaaaatcc
    18241 aaatccatcc gtacgtacgt tcacaaaacc ttttaatgaa gtcggaattc agacacatat
    18301 gctcgaccga ggcctgaatg gtattcgtat gaatccggta ccagccgatg tgcgtcagct
    18361 aatgtacaaa gtgaaaaaag cacagggtgt tgatattacc cgtattttct gcggtctgaa
    18421 tgatgtgcgt aatattgctc tttcaatcaa atatgccaaa gctgccggta tgattgctca
    18481 ggcagcgatg tgcatcacat actccgaagt tcataatgtt gagtattatg ttaatatggc
    18541 agaacagttg attgccgaag gtgctgacga aatctgtctc aaggatatgg caggcatcgg
    18601 gcgtcccgat atgcttggga aaattgtaaa agccattaaa acttcccatc ctgaaattcc
    18661 cattcagtat cacggacatt caggtcctgg tttctcggtt gcttcaatgc tcgaagtggc
    18721 aaaagcagga gttgattacc tcgatgttgc tatggaacca ctttcgtggg gtatggttca
    18781 tcccgatgta atcaccattc aggcaatgct gaaagatgca ggatttaaag tacccgaaat
    18841 caatatgaaa gcctatatgg aagcacgccg cttgacccaa agctttgtgg acgatttcct
    18901 gggatatttc atcgatgaaa gaaataaata tatgacatcc ctgctcatcg gttgcggtct
    18961 gccgggcggt atgatgggct cactcatggc cgacctcaaa ggcgtacatg caggtatcaa
    19021 tacctatttg aaaaacaata ataaacccga actttcgaca gacgagatgc tggttatgct
    19081 tttcgatgaa gtgggatata tctggcctaa actcggaaat ccacccctgg taactcctta
    19141 tagtcagtat gtaaagaata tcgcgctcat gaatgtgatg gctatgatca agggtcagcc
    19201 acgctggggt atgattgaca aaaactcctg ggatatgatt ttgggtaaag ccggaaaact
    19261 gcccggaaaa ctcgatccgg aaattatcga actggctaaa aagaacaact acgaattctt
    19321 cgaaggcaat ccgcaggaca attatcccga cgaacttccg aaattcatca aagaaatgga
    19381 aaaacttgga tgggatagag gtcaggacga cgaagaactc ttcgaatttg ctatgcacga
    19441 aaaacagtac cgtgatttca aatccggaat ggcaaaagaa cgttttgata aagagctcga
    19501 agctgctata aaggaaaaat atgcacaaag tgaagttcaa ataccggata tccgtgctct
    19561 caaatatccg aatgcagaac ctgtagtttc aaccgtttcg ggtcgccttc tctgggaact
    19621 cgatttcaat gaagtatcgg ttgaacctcc tcatggaaaa catttcaaac aatttgacat
    19681 gatttgtgct atacagacga attatggtgt ggaaccaatc ggaagtttct gcaatggccg
    19741 tattgtcgga gtagtgaaaa atcagggtga tctggtacgt aaaggcgaag cactcgctta
    19801 tatcgaaaag gactgattga aaagaattca taagttaaac aaaaaaagaa tggaaatcgc
    19861 atctggtttt cattcttttt tttgtgtaat gtcagctaat tctgataaaa atcatatatt
    19921 tgtacccaat agcggataaa aagcaaaaaa tgtttccgaa aaagcaacaa aatgttttat
    19981 ttaatagtaa gaatatatga ttaaatccct gtttatccga aaatccctgg ctcaaatcga
    20041 aactcaggct gaagaaagca gtctgcgaaa acacctcaat cttaccaata tcatcctgct
    20101 gggcatagga tgtattatcg gagcaggaat ttttgtcctt accggaactg ctgcggcttt
    20161 gcatgcagga cctgccattg caatttcttt tatcgtgtcg gcatttggtt gtcttttggc
    20221 gggtctctgt tatgcagaat tttcatccat gatacccgtt tcgggcagcg cttacaccta
    20281 tggttatgcc accatgggtg agtttatcgc ctggattatc ggatgggatt taattctcga
    20341 gtacctgttc gggtctgcaa ctgtggcagt aggatggtcg gggtatgtaa ccagtttcct
    20401 gagcgacata gggcttcaac taccagcatc catctgtcaa agtcctttta ttttcgatgc
    20461 ttcaggctgg catgcaaccg gtgcattaat aaactttccg gcagttttca ttgtagtatt
    20521 gatgaccaca ttactcgtaa taggtatcaa agaatcggcc agtttcaata acatcatcgt
    20581 attaataaag gttactgtta ttctgctttt tatcggattt gggatagcac acatcaacgt
    20641 ggacaactgg acacctttta tacctgcaaa tacaggaaat ttttcacaat ttggatggac
    20701 aggtatttta accggcgcag cagtggtgtt tttcgcgtat atcggtttcg atgcagtttc
    20761 aactacagcg caggaagcta ttaatcccaa acgcgatatt ccaatcggta tcttaggttc
    20821 gctggttgtc tgtacgatct tatatgtagc tgttagtctg gtgatgaccg gaattgttaa
    20881 ttataaggac ctgaatgtgt ctgcacccat tgcggttgcc attgatgcag ccgggaaaag
    20941 cctccgttgg ctcagtccga taattaaaat cggagctatt gccggactta gttcggtagt
    21001 tatggtgctt cttctggcac agtcccgcat tttttacacc atggcaagtg atggactgct
    21061 ctggaaaagt tttgcaaaaa ctcatccaaa attcaaaact ccttatatta caagcatcgt
    21121 gaccgggtgt tttgcagcgc ttttcgcagg tttatttccc atcggacttc tgggcgagct
    21181 ggtttccatc gggacactgc ttgcctttgt cattgtcagt gtcggactga taatcctccg
    21241 aaaaactgag ccggatgctc accgggcctt ccgtaccccg tttgtacccc tggttccgat
    21301 tttaggagcg ctggtttgtt tggttcagat ggcatcactt ccactcgaca cctggatacg
    21361 gcttttaggc tggatggcaa tcggttttat tatctatttt acctatggaa ttaagcacag
    21421 ccgggtgaga aaagaaaaac aaaacaatca gtcagtttga tgagcacatt ataagaaaag
    21481 ttcgaaaaaa aatccgcaga accattgttc tcggtttttt ttttagattt acaggtgaat
    21541 taatacggtt gatgaaaaaa atacagatcc taaacgataa aataagcaaa cagacaaata
    21601 atccagcatg taaaatcctt tcgcaagttg gattaactga ttaattattt gcagattaat
    21661 aaatttgttg tacctttgcg gactattttt aagcaacata atgtctaacc tactatttat
    21721 tagtaaaaaa caaaatttca gaaatggaag aaaatgaaaa attagctgaa gaagttaacc
    21781 aaactcctga agctgttgaa gaaacacaac aacctgccgt agaagaaact gctccagtag
    21841 ttgaagaagc tgcacccgtt gcagaagaag taattgaaga agcacctgct gcaaaaaaag
    21901 aaacacccaa gtatgtgaaa cccaccggac cggctgctga tttcgattgg gaagcctatg
    21961 aaaaaggtga agtacttaca ggaaaatcaa aagacgaatt agaaaaaatt tacgatgaca
    22021 cactgaatgc tatcaaagac aaagaagttg tggaaggtac tgttacctct atcaacaaac
    22081 gtgaagtggt agtaaatgtt ggatttaaat cagacggcgt ggtttcgatg agcgaattcc
    22141 gctacaaccc agatttgaaa gttggtgata aagttgaagt gtacatcgaa agccaggaag
    22201 acaaaaaggg tcagcttatc ctttctcaca aacaggcacg cgctacccgc agctgggacc
    22261 gtgttaatgc tgctctcgaa agtcaggaag tagttaaagg ttttgttaaa tgccgtacca
    22321 aaggcggtat gattgtagat gtatttggaa ttgaagcgtt cctgcccggt tctcagatcg
    22381 acgtgaaacc tattcgcgac tacgatatct ttgttaacaa aacaatggaa ttcaaggtta
    22441 tcaaaatcaa ccaggaattc aaaaatgttg ttgtttcgca taaagctctt atcgaagaag
    22501 aaattgaaca acagaaaaaa gaaattatcg gtaaactgca aaaaggtcag gtactcgaag
    22561 gaactgttaa gaacatcact tcttacggtg tattcatcga cctgggcggc gtagatggtt
    22621 taattcacat taccgacctc tcatggggac gcgtttcaca tc
//
LOCUS       DAOPYB010000030        21284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2538_length_21284_cov_10.442979, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000030 DAOPYB010000000
VERSION     DAOPYB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21284
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2538_length_21284_cov_10.442979"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /gene="pulA"
                     /locus_tag="PKH58_03965"
     CDS             complement(<1..1105)
                     /gene="pulA"
                     /locus_tag="PKH58_03965"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HNX88215.1"
                     /translation="MLLMAGLLVGCGTNYKKYKSYDEYPVYNGEDLELTYSPSGSKFR
                     VWSPAADEVKLLLFDNGLEGAAYMMKDMHRSKNGTWTVAVSDDLKGKFYTFQIKIGEK
                     WMNETPGMWVKAVGINGKRGAIIDFKETNPEGWENDKRPELKSFADITLYEVHMRDFS
                     VSPTSGMKYKGKFLAFTEHGTKNTAGQATGIDHLKELGITHVHLLPSFDYASVDETKL
                     NENKYNWGYDPMNFNVPEGSYSTDPANPVARIKEFKQMVQSLHKSGIRVVMDVVYNHT
                     FAGEESHLNQLVPGYFYRFNKDSTWSNASGCNNETASERPMMRKFIVESVVYWAKEYH
                     VDGFRFDLMGIHDIETMNAVRAALDKVDPTIVIY"
     gene            complement(1253..3316)
                     /locus_tag="PKH58_03970"
     CDS             complement(1253..3316)
                     /locus_tag="PKH58_03970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNX88216.1"
                     /translation="MTVLTAAGSAIAGNPFFKPYNTPHQTIPFNELKTEYYIPSFEEA
                     MIQHKAEINKIVNNPKAPTFENTIVALDNSGELLTKVSSPFYNLLSAETTDEMQNIAQ
                     ELSPKLTEHSNSISLNEKLFARVKAVYEQKDKLKLTPEQQKLLKDTYDGFADNGANLS
                     EADKVTYRELSKELSMLTLNYGQNVLKETNKYQLVITDKELLKGLPADVLDMLAENAQ
                     KAGKTGWLINLKATSYVPVMKYADNRDLRREIYMASATKCMVGGEYDNRENLRKIANI
                     RLKIANLLGHKSYADYALVHRMAENKENVYELLNKLLTAYKPAAEKEYAEVQEYANKN
                     GAYFKVQPWDWSYYSEKLKAEKYDLNDEMTKPYFELEKVKQGIFWLANKLYGLKFTEN
                     PAIQVYNPEVKAYDVTDADGKFVAVFYADYHPREGKRAGAWMNDMKGQWMEGKKDSRP
                     HIVNVMNFTRPTATKPALLTFDEVTTMLHEFGHALHGMLTKTNYRSLSGTNVYRDFVE
                     LPSQIMENWAVEKEFLDNFAVHYQTGEKMPAELIKKIKDADNYNIGYLALRQLSFGLL
                     DMKWHTLEQPYEGDVLAFEKEAMEPAQVLPVVENTAMSPTFSHIFSGGYAAGYYSYKW
                     AEVLDADAYSVFQKNGIFDKKTAASFRKNILEKGGTEHPMTLYKRFRGQEPDIEALLK
                     RNGIK"
     gene            complement(3363..4721)
                     /locus_tag="PKH58_03975"
     CDS             complement(3363..4721)
                     /locus_tag="PKH58_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX88217.1"
                     /translation="MRLLKPFSLLMLMTTLLMSVIQTFAQTDIDPLITEVQVTDTDST
                     YRYLYYYNDYKEKTLETKYVLKGDEWVRRNQTEWLYSNGLCTLQRMNRWNNNNWETFY
                     HIIYEYSNGNLIKVTHQNVVNGNTILISKSECSYNQHLLTNKTDYNWSGDYWIKTLNT
                     DYLYNEKDLAEQITIYRYSSGFLSGVSKINFSYNDSAKVINQLFSEKTDTSWVNVTKS
                     ETYYLPDGKIKSSEIQKSWDKTSNKWVNSQNVFYTYDSANRLTEELYQSWKNVFWENE
                     LQYRYEYNSDGAVLKKETCLPVYDDFRTVSSINYTDFKYSRASLIKSNIEFWGGDAGT
                     VISTFIPFQFNQETIIRKAGQIKISYIPVSDTGISNVKNNLSGEIRVYPNPSKGIFYF
                     DSQQVDVNQWLVTDLSGKTLMRKNQQERSGVIDLGDFNRGIYLLYVVTPQGVRVQKLI
                     RE"
     gene            4953..5609
                     /locus_tag="PKH58_03980"
     CDS             4953..5609
                     /locus_tag="PKH58_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNX88218.1"
                     /translation="MKKIFTPYIKSLDAADIETAADVLEENGTKSVIDTLNWPDQYPY
                     KPITYFYIGRSADAVFIKYSVKGTMLKAVYSEDNSPVHEDSCVEFFCMPEGAAKYTNF
                     EFNCIGTCSASSRRSRNEDIVLFSPNEMATIQRYPSMGRRAFKEMEGMFEWELTVKIP
                     MKLMNIDPENLPEKIRVNFYKCADDTDSMHFVSWAPIDTENPDFHRPEYFGELYFTEK
                     "
     gene            5776..7158
                     /locus_tag="PKH58_03985"
     CDS             5776..7158
                     /locus_tag="PKH58_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNX88219.1"
                     /translation="MKKIITGTLILGACSSFLAAQNNFPNVIFILADDLGYSDIEPYG
                     QEKIKTPNLTAMTRNGMQFMQFYAGTSVSAPSRASLMTGLHTGHTRIRGNFEHIPEGQ
                     EPVGNLQTLADLFHQAGYVTGCFGKWGLGYPNSGSEATDKGFDEFYGYNCQRKAHTYY
                     PQWLYDGKTKVLLNGKSYSQDLIHQKALDFIRKNKDRSFFGYFTYTLPHAGLEQPNDS
                     ILKMYSGKYPEPKAFKGYGDYADTPEPRAQFAGMVTRLDTYVGQIRNELEKLGIAENT
                     LIIFTSDNGPHLEGGADPAFFACDKTLRGTKRSLYEGGMRVPMIAEWKGTIKPGTISE
                     LPAAFWDMMPTFADITGQQKSWIQTTDGISFLPELKGTGTQTKHKYLYWEFHEENGRQ
                     ALRQGDWKLIRQNIRSGNPTLELYNLKSDPFEKNNLAASKSNRVNSMKKLLDKARTHS
                     ELFNFGINEE"
     gene            7168..9720
                     /locus_tag="PKH58_03990"
     CDS             7168..9720
                     /locus_tag="PKH58_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNX88220.1"
                     /translation="MKKVFQLLTFMTISVLIYSQNSATISLNSGWQFSEAGKNEWKDA
                     VVPGSVQSDLIRLGVLPDPYYGTNENVVQWPEEKDWDYKQTFTVTPEQLKSDEALLVF
                     EGLDTHADVFLNGSRILQTQNMFVGHKISVKNSLQAGENKLYIRFYSPLKLMEPARLT
                     AGYEYPAGNDHSDKKVSIFNRKAPYQFGWDWGIRIVQMGIWKPVSLQFYNQACIDNLY
                     VKQLSVSKNQALTDNQIEILSTSEIPVKIVTGAVLNGREIQTAEKQTILKKGKNKVCI
                     ALTIDNPELWMPSGWGKQNRYDFYAKVYSGERLIAEKTEKTGLRKVQLVQQPDEHGKS
                     FYFEVNDIPLFAKGTNYIPGEILRTQQDKTYYDRLFDNLTEANMNFIRVWGGGTYEDD
                     YFYQLADEKGILVWQDFIFGCVPYPSDDAFLANVKEEVIYNVKRLRNHPSLAFWCGNN
                     EIDEGLDHWGWDKEYSAEINKEWREGYNKTFRKLIPELVNENDGTRSYIHGSPYDSNW
                     GNLASFSSSDVHDWGLWHGRMPFEAMTDRLPRFASEFGFQSFPEMKTIRTFATEKDFD
                     INSDVMKIHQKSGIGNTVIKQYMDMYYHAPKNFEDFVYVGLVMQGNGMKEAVEANRRG
                     RPYCMGALYWQLNDDWPVVSWSSVDYYNNWKAQHYKMRDVFAPVALGSVIKDNKLSYL
                     TMSDKLEDVNNLVLNIQVIDFSGKKLKSFSEAVTAKANTSRIVKTFDTDLLLTEDQKH
                     NCVIHAWLSDKNGKIVSVKDYYFFWPNKLNLPQTTISKTVKYEDGKYTITLSSPKLAK
                     DVFVEIPVQGARFSDNFFDLMPGQKKVITIISPELKKIKKTDITIRHLRETY"
     gene            9851..10603
                     /locus_tag="PKH58_03995"
     CDS             9851..10603
                     /locus_tag="PKH58_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HNX88221.1"
                     /translation="MYKLEICANSVASCVEAQKGGASRVELCAAIPEGGTTPSYGEIA
                     VARELLDISLHIIIRPRAGDFLYSRTEHKIMLKDIAIARKLGADGVVIGCLTPDGDVD
                     MIRNRELIDAAKGMSVTFHRAFDMCRNPLESLEKIIELGCDRILTSGQQPKAEQGIDL
                     LKQLVTIADKRIIIMPGSGINENNIAYIAHETGATEFHLSARSAIPSQMQYRNPNVSM
                     GGKGIEINEYEQNTTNAVKVNKTLENLNNQLI"
     gene            10647..12938
                     /locus_tag="PKH58_04000"
     CDS             10647..12938
                     /locus_tag="PKH58_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HNX88222.1"
                     /translation="MIVMLSGLTLKAAEANFRVIPLPNEIKSTNAGAFNLNASTRIIY
                     PKGNEMMKKNAEFLSDYLKEITGIKPDVSDSGSKSNAIVLENGLKHANPEAYKLTVTS
                     KNIVIAGAGERGVFYGIQTLRKATPIGTAKISYPAVTILDSPRFAYRGMHLDVGRHFM
                     SADFVKKYIDILAMHNVNTFHWHLTEDQGWRIEIKKYPKLTEIGSMRAETVIGHNTGK
                     FDGKPHGGYYTQEQIKDIVAYAQARYITIIPEIDLPGHMLAALAAYPELGCTGGPYEV
                     EKTWGVFDDVLCPGKEKTFTFLEDVLTEVMALFPSKYIHIGGDECPKVRWTKCPDCQA
                     KIKELGLKDTDKHKKEFYLQSYVTERIEKFLNKNGRSIIGWDEILEGDLAPNATVMSW
                     RGIEGGIEAAKLGHDVIMTPNSHLYFDYYQTADTENEPMAIGGFLPLERVYSYEPLPD
                     TLTPEQKKHILGPQANIWTEYIPDSKQVEYMLLPRLAALCEVQWTQPEKKDYASFIAR
                     LPHMIQLYDLYKLNYATHVFDIQANLTPRPEKGSLEASFKTIDNAPVYYTLDGSEPTT
                     KSSLYSGPVSLNQNGTIKAKAFRTNGKDSRTFTQTINLGKASLKPIKLLTKPARNYGY
                     TGASLLNDGLSGKGTNYKTGRWIGFSGDNLEAVIDLLKTTEISRASIKNCVVTGDWIL
                     DAQAFTVEVSSDGENFTYAAGLETNPSDKTEHWKGIVTHTVTFRPVTARYVKVTVKPF
                     TAFPAWHGGDGKPYIFVDEISVD"
     gene            13265..14566
                     /gene="murF"
                     /locus_tag="PKH58_04005"
     CDS             13265..14566
                     /gene="murF"
                     /locus_tag="PKH58_04005"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNX88223.1"
                     /translation="MELYEIFKKHPVICTDSRNCPEGSLFFALKGDNFDANAFALSAL
                     EKGCAYAVIDDKQYAVDERFILVNNVLETLQDLARQHRRQLGTTIIGITGTNGKTTTK
                     ELIAATLKQKYRIHFTEGNLNNHIGVPLTLLQLTPEHELAVVEMGANHPGEIKVLADI
                     AQPDYGIITNVGKAHLEGFGSFEGVMRTKGELYDYIYKSGGKIFLNTGNAFLCSMSKK
                     AKFISPDRILSYELGNNLKGHVSVGKTLSCNPLLHLKCQNKESEFEIRTQLIGTYNAE
                     NVLSAATIGTHFGVSNELIKKGLEEYIPRNNRSQLTISEHNKIIADAYNANPTSMHAA
                     ILNFAQMDVPEKTLILGDMLELGKQSADEHQKIADLVKDKGFSSVYLVGKSFQATKSN
                     FKKFADIGALIAEIRENPLQNKFILVKGSHGIHLEKILELI"
     gene            14591..15487
                     /locus_tag="PKH58_04010"
     CDS             14591..15487
                     /locus_tag="PKH58_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88224.1"
                     /translation="MEKKKMTILLVALGVVIAALIAGLLYTMNASSEKDEQIAATEEM
                     MTSEKQQLETEYKDLAGEYDGYTMTIHNDSLLKLFNEEKLKVNKLLEELKTTKSSNAK
                     RIIELKKELAALRKVMVHYVNQIDSLNLQNKRLTNENYEVRQKYASASATAEQLSKEK
                     ESLNEVVTRAAILEVYNFSMTPLDKRNKKTDRASKITTLQFNYTIGKNITATPGQKTV
                     YVRLTRPDDELMTKSNDSYFTYENKQIACSLSKDFEYSGEARNDVLYWKVDEILQIGN
                     YRADFFADNNRIGTFTFRIEKN"
     gene            15521..>16107
                     /locus_tag="PKH58_04015"
     CDS             15521..>16107
                     /locus_tag="PKH58_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNX88225.1"
                     /translation="MTAESRKFGIAAGKIAFDEKHRSTIKFNISRYEAAFAKGLARYK
                     NVNSAKSTAAAIKRDVLKNWDKYLLEFEKNITANGAEVLWAENETEATAYIRKILTEN
                     EARLLVKSKSMTTEEIELNETAKSLGVESVETDLGEFIVQVAGEKPYHIVTPAMHKSK
                     KDIAVLFNEKFNTPADSTPEELTDYVRGVLRKKYTA"
     assembly_gap    16108..16207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16208..16759
                     /locus_tag="PKH58_04020"
     CDS             <16208..16759
                     /locus_tag="PKH58_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX88226.1"
                     /translation="EADGPEKMYVILLDNGRTTIFADDEARESLACIRCGACLNGCPV
                     YKTIGGYTYDSTYSGPIGSVITPFLKGFKDFSHLSFASSLCGRCNEVCPVRIPLTDIL
                     LANRRKAVEQGLRPKAEKWVMKGYRYISSKRAAFDFFSPGLKNIGVAPFNYFGWGPHR
                     TMPSFSKKSFSETYRYKYKNLIK"
     gene            16759..17235
                     /locus_tag="PKH58_04025"
     CDS             16759..17235
                     /locus_tag="PKH58_04025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX88227.1"
                     /translation="MKTNFTKNILILLGISVFSFLFTSCEKEDKYIDWKVLNERWLEQ
                     HKNDPGFKVTESGLCYKVIRQGNPNDRKATSTSYIYVNYEGKLINDSIFDKKEQFQDF
                     LSEMVDGWKEGIIKMHTGDIYQFYIPWELGYGKDGFGSIPPYSTLIFQVELVYSVN"
     gene            17246..18568
                     /locus_tag="PKH58_04030"
     CDS             17246..18568
                     /locus_tag="PKH58_04030"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNX88228.1"
                     /translation="MKRAGFILLFGLLLGVISAQEYTVESVPNPITLNAHHYVSNPDG
                     ILSESTVNALNVKLDSLAQTNGAQVAVVVLNSIGYEDINNFAWSLFDKWRIGKEKEDN
                     GLLVLFVLDQKKVRFENGYGLEGVIPDAIAKRIQTTAMIPEFRNGNYDAGMIAGVNSI
                     ISAINREPVAEEAVKPAFQWDEVLPYALAAYILLSLLTFLWTRSVVRQAKKNNTLTSN
                     LSRYKVIRENNNSVASMIGLYLPLIALALILFFASAVYLIFLLPVPLTVLPSYLYGRR
                     QMTKVRRSPIPCKVCGNQMHILSEKEDDAHLRLSQQLEEELKAVDYDVFVCESCKEKA
                     IFTLDKKSQYETCPRCSTKAFILDSKRMIVAPTYVHAGTERLTYKCKFCGYQENNNRN
                     LPRLKRTNSGFFGGAVGGGMFSGGGGFGGGGGSFGGGMSGGGGATSGW"
     gene            18635..19219
                     /locus_tag="PKH58_04035"
     CDS             18635..19219
                     /locus_tag="PKH58_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNX88229.1"
                     /translation="MKKTGIIIIIALAAILFLWGMGGYNGLVNSQENVKSQWGNVENQ
                     YQRRADLIPNLVETVKGYAAHEKSTFEEVIAARAKATQITIDPTNLTPEKLQEFQAAQ
                     GQLGSALGRLIAVAENYPDLKANQNFLELQAQLEGTENRISVERNKFNEMAKEYDVAI
                     RRFPKNILAGIFNFENIPYFTAQEGAEKAPQVKF"
     gene            19535..20527
                     /locus_tag="PKH58_04040"
     CDS             19535..20527
                     /locus_tag="PKH58_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX88230.1"
                     /translation="MSYTPSPDRYTNSVGYNYCGKSGLQLPRLSMGLWHNFGDVNDLK
                     EARKMLETAFDNGITHFDLANNYGTPFGSAEANFGKILKEDFAPYRDELIISTKAGHT
                     MWDGPYGDWGSRKYLIASLNQSLKRMGLDYVDIFYSHRYDPHTPLEETMGALSDMVRQ
                     GKALYVGISKYPTDKARQAYRILADNGTPCLINQDRYNILTRDIEQTVLPLAKEKGIG
                     FIAFSPLAQGLLSNKYLKGIPENSRAAQSYGFLQKDQITPQLVSKLIKLNEFAKKRGQ
                     SLAQMALAWCLHDSQVNSVIIGTSSVKQLKDNLGALKNTHFTDEEMWLIGEIAG"
     gene            complement(20601..21071)
                     /locus_tag="PKH58_04045"
     CDS             complement(20601..21071)
                     /locus_tag="PKH58_04045"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNX88231.1"
                     /translation="MSEHEKFMRKAIALSVRNVKKGGGPFGAVIVKEGKIIATGVNHV
                     TGSNDPTAHAEILAIRKASKKLHNFSLEGCTIYTSCEPCPMCLGAIYWAHLDKIYYGN
                     TKAEARDIGFDDSFIYDEIALKPEQRKVETHHLLADEATDAFKLWKDKEDKIEY"
BASE COUNT         6435 a         4587 c         4502 g         5660 t
ORIGIN      
        1 catagataac aatggtagga tcaactttat cgagcgccgc gcgaacagca ttcatggttt
       61 cgatatcatg gatacccatg aggtcgaaac ggaaaccgtc cacatgatat tccttagccc
      121 agtaaaccac cgattcgacg atgaatttcc gcatcatggg acgttcggaa gcggtttcgt
      181 tgttgcaacc cgatgcattc gaccaggtac tgtccttgtt gaaacggtag aagtatcccg
      241 gtaccagctg gttgagatgc gactcttcac ccgcaaaagt gtgattgtaa actacatcca
      301 tcaccacccg gatacccgac ttatgcaatg actgaaccat ttgcttaaat tccttgatgc
      361 gtgccaccgg attggcagga tcggtcgaat agctgccttc gggcacattg aaattcatcg
      421 gatcgtaacc ccagttgtat ttgttttcgt tgagtttggt ttcatcgacc gaggcataat
      481 cgaaagaagg aagaaggtga acatgcgtaa tgcccagttc tttgagatgg tcgataccag
      541 tagcttgtcc ggcagtattt ttggtgccat gttcggtaaa agccagaaat tttcccttgt
      601 atttcattcc cgaagttggt gatacggaaa aatcgcgcat atgcacttcg tacagtgtaa
      661 tatcggcaaa acttttcagt tccggacgtt tgtcattttc ccatccttca ggattggtct
      721 ctttgaaatc gatgatagca ccccttttcc cgttgatgcc aacggctttc acccacattc
      781 caggcgtctc gttcatccat ttttcaccga tttttatctg aaatgtataa aattttcctt
      841 tcagatcgtc cgaaacagcc accgtccagg ttccgttttt ggatcgatgc atatctttca
      901 tcatatacgc ggcaccttca agcccgttgt caaaaagaag cagtttaact tcatcggcgg
      961 caggcgacca aaccctgaat ttggagccgg agggagagta agtcagttcc agatcctcac
     1021 cgttgtaaac cggatactcg tcataacttt tatacttttt gtaattagtt ccacagccaa
     1081 ccagcaatcc agccatcagt aacataagaa ttgttttttt cattgacgta atatttttcc
     1141 tgattaaaat attttttttc aaaaatatac ttatttcagg atattaccta ataaaaacaa
     1201 aaaagagtgt gaaattgata tttcacactc ttttgttttg atcaagaggt cattacttta
     1261 taccgtttcg tttgagcaat gcctcaatat ccggttcctg tccgcggaaa cgtttgtaga
     1321 gtgtcatcgg atgctcggtt ccaccttttt ccagaatatt tttacggaaa gatgctgctg
     1381 ttttcttgtc aaaaattccg tttttctgga atacagaata ggcatctgca tcgagtactt
     1441 cggcccattt gtagctgtaa tatccggctg cataaccacc agaaaatata tggctgaacg
     1501 tggggctcat ggctgtgttt tcgacaaccg ggagtacctg agcgggttcc atagcttcct
     1561 tttcaaaagc gaggacatcc ccttcgtaag gctgttcgag ggtgtgccat ttcatatcca
     1621 gcaatccgaa actcaactga cgaagcgcca gataaccgat attgtaattg tcggcatctt
     1681 taatcttttt aattaactcc gcgggcatct tttcgccggt ctgataatgt acggcaaagt
     1741 tgtcgagaaa ttctttttcc acagcccagt tttccataat ttgagaggga agttccacaa
     1801 agtcgcgata aacattcgta cccgacaatg aacggtagtt ggtttttgta agcattccgt
     1861 gcaacgcatg tccgaactcg tgcagcatgg tggtaacctc atcgaaggtg agcagtgccg
     1921 gttttgtagc cgtaggacgg gtgaaattca tcacattaac tatatgcgga cggctgtctt
     1981 tttttccttc catccactga cctttcatat cgttcatcca ggcgccggca cgtttgcctt
     2041 cgcgcggatg atagtcagcg taaaacacag ctacgaattt gccgtcggca tcggttacat
     2101 catatgcttt cacttcggga ttatacacct gaatagccgg attttcagta aatttcaagc
     2161 cataaagttt gttggcgagc cagaatattc cctgttttac tttttcgagt tcaaagtagg
     2221 gtttggtcat ttcgtcattc aggtcatatt tctcggcttt cagtttttcg gagtaatagg
     2281 accagtccca gggctgtact ttgaaatagg ctccattttt attggcatat tcctgcacct
     2341 ctgcgtattc tttttcggct gcgggtttgt aggctgtaag cagcttattg agtaattcat
     2401 atacattttc cttgttttcg gccatgcgat gaaccagggc gtaatcggca taagatttat
     2461 gacccagcaa gttggcaatt ttcagacgta tattggcaat tttacggaga ttttcgcggt
     2521 tgtcatattc ccctccgacc atacatttgg tcgcacttgc catgtagatt tcgcggcgca
     2581 ggtcgcggtt atctgcatat ttcattaccg gcacatagct tgtggctttc agattgataa
     2641 gccagcctgt ttttccggct ttctgagcat tctcggcaag catatcgagc acatcggcgg
     2701 gtagtccttt cagcagctct ttgtcggtaa tcaccagctg atatttattg gtttctttca
     2761 gcacattctg tccataattc agggtaagca tactcaattc tttggaaagt tcgcggtagg
     2821 tgactttgtc ggcttcggac agattggcac cgttatcggc aaaaccatcg tatgtatcct
     2881 taagcagctt ttgctgttcg ggagtaagct tcagtttgtc cttctgttca tacacagctt
     2941 taacccgggc aaaaagtttt tcgttcagac tgatagaatt ggaatgttcg gtcagcttgg
     3001 gagacagttc ctgagctatg ttttgcatct catcggttgt ttcggcgctg agcaggttat
     3061 aaaacggtga cgacactttt gtaagcaatt cgcccgaatt gtcgagcgca acaatggtat
     3121 tttcaaaagt cggcgctttc gggttgttta ctattttatt aatttcagct ttgtgctgta
     3181 tcatcgcctc ttcgaaggag gggatgtagt attccgtttt cagttcattg aatggaatgg
     3241 tttgatgcgg agtgttgtaa ggtttgaaaa atggatttcc ggctatcgct gaaccggcag
     3301 ctgtcaatac agtcatacta aggattattt tctttaacat atttaattat tttaaattga
     3361 atttattctc tgataagttt ttgaacacgt acaccctgtg gagttacaac atacagcaga
     3421 tatattcccc ggttgaaatc accgagatcg ataactcccg aacgctcctg ctgatttttc
     3481 ctcatgagcg tttttccgct caggtcggtc accagccact ggttcacatc aacctgctgg
     3541 ctatcgaaat aaaaaatgcc tttcgagggg ttaggataaa cacgaatttc accggataaa
     3601 ttgtttttaa cattagatat accggtatcg ctaaccggaa tatagcttat tttaatctga
     3661 ccagcctttc ggatgatagt ttcctgatta aactgaaatg gaatgaaggt tgaaataacc
     3721 gtaccggcat ctccacccca aaactcaata tttgatttta taagactggc tctcgaatac
     3781 ttaaaatcgg tataattaat tgatgaaact gtccggaaat catcatacac cggcaaacag
     3841 gtttctttct tcagcacagc gccatcggaa ttatactcgt agcggtattg cagttcattt
     3901 tcccagaaaa catttttcca gctttgatag agttcttcgg tcaaccggtt ggctgaatcg
     3961 taggtgtaaa atacattctg agagttcacc catttgttac tggttttatc ccacgacttc
     4021 tgtatttctg atgattttat tttgccatca ggcaaatagt aggtttcgct ctttgttaca
     4081 tttacccagc ttgtatccgt tttttcggaa aataactgat taatcacctt tgcagagtca
     4141 ttgtaactga aattgatttt agataccccg gacaaaaaac cggatgaata tctgtatatt
     4201 gtaatttgtt cagctaaatc cttttcgttg tataaataat cagtattcag tgttttaatc
     4261 caataatcgc ctgaccagtt gtaatcggtt ttgttcgtca gcaaatgctg attatagctg
     4321 cattctgatt tcgaaattaa tattgtattt ccgtttacca cattctgatg cgttactttg
     4381 atcagattac cattactgta ctcataaatg atatgataaa aggtttccca gttattgtta
     4441 ttccaccggt tcatacgttg caaggtacac agtccgttgc tgtaaagcca ttcggtctga
     4501 tttcggcgca cccattcatc tcctttcagc acgtattttg tttccagtgt tttctcctta
     4561 tagtcgttat aatagtacag ataacggtat gtgctgtcgg tgtcggtaac ctgtacttcg
     4621 gtaatcaatg ggtcgatatc agtctgagca aacgtctgaa tgacagacat aagcaaagtt
     4681 gtcatcagca tcaggagaga gaatggtttc aacagcctca ttttttgtac aaattaaatc
     4741 tctgtaaata ttataaatct gccaaagata aacacaaatg ttgatttcaa caaaaaacaa
     4801 attcaacctt tagcaatttt aatattccta ctgatatact gataattaga ctgtttttta
     4861 ttcagccggt tttaatgaag ataatttatc cgaaatcgaa aaaaaatgat taacattgca
     4921 gtcagatatg tagatccaaa aacaataatg ctatgaaaaa aatttttaca ccttacatca
     4981 aatcactgga tgctgctgac attgaaacag cagctgacgt gctggaagaa aacggaacaa
     5041 aaagtgtaat tgacacactc aactggccgg atcaatatcc ttacaaaccc atcacttatt
     5101 tctacatcgg gagatctgcc gatgcggttt tcattaagta ttcggtgaaa ggaaccatgc
     5161 ttaaagctgt ttattccgaa gacaattcgc cggtgcacga agacagttgt gtagagtttt
     5221 tttgtatgcc cgaaggtgcc gcgaaataca ccaattttga gttcaactgc atcgggacct
     5281 gttcagcaag cagccgccgc agccgcaacg aagatatagt gcttttctca cccaacgaga
     5341 tggcaaccat tcagcgttac ccttccatgg gccgtcgggc cttcaaggaa atggagggca
     5401 tgtttgaatg ggaactcacc gttaaaattc ccatgaaact gatgaatatc gaccctgaaa
     5461 atcttcccga aaaaatcagg gtaaatttct acaaatgtgc tgacgacacc gattccatgc
     5521 actttgtaag ttgggcaccc atcgatacgg aaaatcccga ctttcaccgg ccggagtatt
     5581 tcggcgaact gtacttcacg gaaaaataat ccttttgcac aaataacgac tgcctgaact
     5641 gacaaaattt caggcagtca tttttatttc ctgtaatatt tacaaaaatt gctgtttttt
     5701 gtaaaaacat tggaacgagt ctttcttttt tcgtattttc gtaccaacaa aaaatactat
     5761 acaaatctac tgacaatgaa aaaaattatc accggaacat tgatccttgg tgcatgctca
     5821 tctttcctgg ctgcacaaaa caattttccg aacgtaatct ttatcctggc agatgacctg
     5881 ggttattcgg acatagagcc ctacggtcaa gaaaaaataa aaacccccaa ccttacagcc
     5941 atgacccgga acgggatgca gtttatgcag ttctatgccg gaacttcagt cagcgcccct
     6001 tccagagcca gccttatgac cggactgcac accgggcata cccgcatcag aggtaatttt
     6061 gaacatatac ctgaaggtca ggaacccgta ggcaacttgc agacccttgc cgacctgttt
     6121 catcaggcag gttatgttac cggatgtttc ggaaaatggg gtcttggata cccaaattcg
     6181 ggttcggaag cgactgacaa aggttttgat gaattctacg gatacaactg tcagcggaaa
     6241 gcacatacct actatccgca atggctgtat gacggcaaga caaaagtcct gcttaacggg
     6301 aaatcctatt cacaagactt aatacaccaa aaagcattag attttatacg caaaaataaa
     6361 gaccgttcct ttttcggata ttttacctat acgcttcccc atgccggact ggagcagccc
     6421 aatgacagta ttctgaaaat gtattccgga aagtatcccg aaccgaaagc tttcaaaggt
     6481 tacggcgatt acgctgacac accggagccg agagctcagt ttgccggaat ggtcacccgc
     6541 ctggacacgt atgtgggaca aatccgaaat gaactcgaaa aactcggaat agctgaaaac
     6601 acgctcatca ttttcaccag cgacaacggt ccgcacctgg aaggcggtgc cgacccggca
     6661 ttctttgcat gtgacaagac gctcagaggt acaaaacgat cgctctacga aggtggtatg
     6721 cgtgttccaa tgatagccga atggaaaggt accatcaaac cgggcaccat cagtgaactt
     6781 cccgcggctt tctgggacat gatgcccact ttcgcagaca tcaccggaca gcagaaaagc
     6841 tggatacaaa ccaccgatgg aatatccttt cttccggaac tgaaaggaac cggcacgcag
     6901 acaaaacaca aatacctgta ctgggaattt catgaagaaa acggaaggca ggcactacgg
     6961 cagggcgact ggaaactgat ccggcagaac atcaggagtg gaaatcccac gttagaactt
     7021 tataatctaa aaagtgaccc ctttgaaaag aacaatctgg cagcctctaa aagtaaccgg
     7081 gtaaacagta tgaaaaaact cctggataaa gcacgtactc actctgaact tttcaatttt
     7141 ggcattaacg aagaataaaa aaacgctatg aaaaaagtat ttcaactgct gacatttatg
     7201 acaatctctg tattgatcta ttctcaaaat tcagccacca tcagtctgaa ctccggatgg
     7261 caattctccg aagccggaaa aaacgaatgg aaagatgctg ttgtaccggg ttcggttcag
     7321 tccgacctga tacgcctggg tgtcctaccc gatccgtatt acggaaccaa cgagaacgta
     7381 gtgcagtggc ccgaagaaaa agactgggac tacaaacaaa ccttcactgt cacaccagaa
     7441 cagctcaaat cggacgaagc gttgcttgtt ttcgaaggtc tggatacaca tgccgatgta
     7501 tttctcaacg gttcccgcat tctgcaaaca caaaacatgt ttgtgggtca taaaatatcg
     7561 gtgaaaaatt cactgcaagc cggtgaaaac aaattataca tccggtttta ttcgcccttg
     7621 aagcttatgg aaccggcacg actgacagcc ggatacgaat atcctgccgg taacgatcac
     7681 agcgacaaaa aagtgagtat cttcaaccgg aaagcacctt atcagtttgg ctgggattgg
     7741 ggaatacgta ttgttcagat gggtatctgg aaacctgtct cactgcaatt ctacaatcag
     7801 gcttgcattg acaatcttta cgtcaaacaa ctatcggtca gcaaaaacca ggcactaacc
     7861 gataatcaga ttgaaatact ttccacgtcc gaaataccgg tgaagattgt gaccggcgct
     7921 gttttaaatg gcagagaaat acaaactgct gaaaaacaaa caatcctgaa aaaaggaaaa
     7981 aacaaagtct gcatcgcatt aaccatcgac aatccggaac tttggatgcc atcgggatgg
     8041 ggaaaacaaa accgctacga tttctatgca aaagtttatt cgggtgaacg gcttatcgct
     8101 gagaaaaccg aaaaaacagg attacgaaaa gtgcagctgg tacagcaacc cgatgaacat
     8161 ggaaaatcgt tctattttga agtaaacgat atcccgttgt ttgccaaagg caccaactac
     8221 atccccggtg aaatactgcg cactcaacag gataaaacct attatgacag gctattcgat
     8281 aacctgacgg aagccaacat gaattttatc cgtgtgtggg gaggcggcac ttatgaggat
     8341 gactatttct atcagctggc tgatgaaaaa ggaatacttg tgtggcagga ttttatcttc
     8401 ggatgcgtac catatccttc ggacgacgca tttctggcta atgtgaagga agaagtcatc
     8461 tacaatgtaa aaaggctccg caaccatcct tcactagcat tctggtgcgg caacaacgaa
     8521 attgacgaag gtcttgatca ctggggctgg gacaaggaat attcagcaga gatcaacaag
     8581 gaatggcggg aaggatacaa taagacattc cgcaagttga tacccgaact ggtaaatgaa
     8641 aacgatggaa cacgaagtta tatacacggg tcgccttatg actcaaactg gggtaattta
     8701 gcaagtttca gctcaagtga tgtgcatgac tggggattgt ggcacggtcg gatgccattc
     8761 gaagccatga ccgacaggtt gccgcgcttt gccagcgaat tcggatttca gtcattcccg
     8821 gagatgaaaa ccatccggac ttttgccacc gaaaaagatt tcgacatcaa ttccgatgtc
     8881 atgaaaattc atcagaaaag cggcattggc aatacggtaa ttaagcaata catggacatg
     8941 tactatcatg ctccaaaaaa cttcgaagat tttgtatatg tcggactagt gatgcagggc
     9001 aatggcatga aggaagccgt agaagccaac cgccgcggca ggccatactg catgggagcg
     9061 ctttactggc agcttaatga cgactggccc gttgtctcgt ggtcaagtgt ggattactac
     9121 aataactgga aagcacagca ttacaaaatg agggatgttt ttgcacctgt agctcttggc
     9181 tcggtaataa aagataacaa actgagctac ctcactatgt ccgacaaact ggaagatgta
     9241 aacaacctcg ttctgaatat tcaggtgatt gatttttcgg gtaaaaaact caaaagtttc
     9301 agcgaagcgg taaccgcgaa agccaacaca agccggattg tgaaaacatt tgacacagac
     9361 ctcttgctta ctgaggatca aaaacataac tgcgtaattc atgcctggct gagcgacaaa
     9421 aacggtaaaa ttgtttcagt gaaagattac tatttctttt ggccaaacaa actgaattta
     9481 cctcaaacaa ctatcagcaa aacggtgaaa tacgaagacg ggaaatacac cataactttg
     9541 tcatctccca aactggcgaa agatgtattt gtggaaattc cggtacaggg ggcacgtttc
     9601 tcggacaatt tctttgactt gatgcccgga caaaagaaag tgatcacaat catctcgccg
     9661 gaattgaaga aaataaaaaa aacagatata actattaggc atcttagaga aacgtattaa
     9721 ggtgattagt gactagtgat tgacttattg gggtcttagg tcttttatct attgtactgg
     9781 ctcttggttt tattgcctaa tgtcttggtt cttggttcta ttagtctttg ttctataaat
     9841 cttacaaaaa atgtataaac tggaaatttg cgccaattca gtggcgagtt gcgtggaagc
     9901 acaaaaggga ggtgcatcga gggttgaact ttgcgctgcc attcccgaag gaggtacgac
     9961 tccctcctac ggtgaaatag cggtagcacg cgagctgctc gatatcagcc tgcatatcat
    10021 catccggcca agggcgggag attttcttta ctcccgaacc gaacataaaa tcatgctcaa
    10081 agacatcgcc attgcccgta aacttggggc tgacggtgta gttatcggtt gcctgacccc
    10141 cgatggcgat gtggatatga ttcgtaacag ggaactgata gacgcggcta aaggcatgag
    10201 cgttactttt caccgggctt tcgatatgtg ccggaatcca ctggaaagtc tggaaaaaat
    10261 cattgagttg ggatgcgaca gaattctgac atccggtcag caacccaaag ccgaacaagg
    10321 cattgatttg ctgaaacaac ttgtcacaat tgcagataaa cgtattatta tcatgcccgg
    10381 aagcggcatc aacgaaaata atatcgcata cattgcacac gaaacaggag ctaccgaatt
    10441 tcacctttcg gcccgaagcg ccataccaag tcagatgcag taccgcaacc cgaacgtatc
    10501 aatgggtggc aaaggaattg aaatcaatga atacgagcaa aacaccacca acgcggtgaa
    10561 agtaaacaaa acacttgaaa atcttaataa tcaactaata taaaattact atgaaacaaa
    10621 aaactttcaa atttctgatt acgtttatga ttgtaatgct gagtggtctg acccttaagg
    10681 ctgccgaagc caacttccgg gttattccgc tgcccaatga aattaagagt actaacgccg
    10741 gtgcttttaa tctgaatgct tctacccgca taatttaccc gaaagggaat gaaatgatga
    10801 aaaaaaatgc cgaatttctg tcggattacc tgaaagaaat tacaggaata aaaccggatg
    10861 taagcgattc cggctcaaaa tcaaatgcca tcgttcttga aaatggtttg aaacatgcaa
    10921 atccggaagc ctataagctg acagtaactt caaaaaacat cgtcattgca ggagcaggtg
    10981 aaagaggtgt attctatggc atacagactt tgcgcaaagc tacacccatc ggcacggcta
    11041 agatcagcta tccggcagtc accattctcg attcgccccg cttcgcttat cgcggcatgc
    11101 atctggatgt gggtcgccac tttatgtcag ccgattttgt aaagaaatac attgacatac
    11161 tggcaatgca taatgtcaac acctttcact ggcacctgac cgaagatcag ggatggcgca
    11221 ttgaaataaa aaaatatccg aaactgactg aaattggttc tatgcgtgca gaaacggtta
    11281 tcggacataa taccggcaaa ttcgacggaa aacctcacgg aggctattat actcaggaac
    11341 aaataaagga catcgttgcc tatgctcagg cacgctacat caccatcatt cccgaaattg
    11401 accttcccgg acatatgctg gcggctctgg cagcttaccc cgagttggga tgtacgggag
    11461 gaccttacga agtagaaaaa acctggggag tctttgacga tgtactctgc cccggcaaag
    11521 aaaaaacatt tacctttctg gaagatgttc ttaccgaagt aatggcactt ttcccctcaa
    11581 aatacatcca tatagggggt gacgaatgcc cgaaagtacg ctggacaaaa tgccccgatt
    11641 gtcaggcaaa aattaaggaa cttggcctga aagacaccga caaacacaaa aaagaatttt
    11701 acctgcaaag ctacgttacc gaacgaattg aaaaattcct caacaaaaat ggacggagta
    11761 ttatcggctg ggacgaaatt ctggaaggcg acctcgcacc caacgccaca gtaatgtcat
    11821 ggcgcggcat cgaaggggga atagaagctg ctaaactggg tcacgatgtg attatgaccc
    11881 ccaacagtca cctctatttt gactattatc agaccgccga tactgaaaat gagccgatgg
    11941 ccatcggagg atttctaccg ttagaacgag tttacagcta cgaaccgtta cctgatacat
    12001 tgactcccga acagaagaaa catattctgg gccctcaggc aaatatatgg actgaataca
    12061 tcccagatag taagcaggtg gaatacatgc tgctaccacg acttgcagca ctttgtgaag
    12121 ttcaatggac acaacccgag aagaaagatt acgccagttt cattgcccgc cttccgcaca
    12181 tgattcagct ctacgatctg tacaaactca attatgctac acatgttttt gatattcagg
    12241 ctaatttaac gccaagacca gaaaaaggaa gtctcgaagc ctccttcaaa accatcgata
    12301 atgcaccggt ttattatacg ctcgacggtt cggagccaac aacaaaatcc agtctttaca
    12361 gcggtcctgt cagtcttaat cagaatggaa cgattaaagc aaaagctttt cggacaaatg
    12421 gaaaagacag ccgtacattt acccaaacga tcaacctagg taaagccagc ctgaaaccca
    12481 ttaaactgct cacaaaacct gcccgtaatt acggttacac aggcgcatct ctgctcaacg
    12541 acggtctttc cggaaaagga accaactata aaacaggacg atggatcggt ttctcgggag
    12601 ataatctgga agcagttatc gatctgctga aaactactga aatatccaga gcttccatta
    12661 aaaactgtgt ggttaccggt gactggatac tcgatgcaca ggcatttaca gtagaagttt
    12721 cgagcgacgg agagaacttc acttatgctg cgggcctgga aacaaatcca tcagacaaaa
    12781 ccgagcactg gaaaggtata gtaacgcaca cggttacatt tcgccctgtc acagccaggt
    12841 atgtaaaagt aaccgtaaaa ccttttacag catttccggc atggcatggt ggagatggca
    12901 aaccttatat tttcgtagat gaaataagcg tagattaaaa aacattcaac atttaattaa
    12961 tgagactgat acaaaaacaa aaaatgtgtc agtctcatta attttattta agaagtaaaa
    13021 gtcatccatc aaaaaaataa tctgataatc tgacaacaaa tcactaaaaa cagggatgat
    13081 tagttatttc atttttgcga aaatgaatcc tggaatctac cttcacgatt tccttccaaa
    13141 caggttgaat ggcatttgat aataaaacag aaaagttcag ctaaaatctt gtttttttct
    13201 taataaaagt tccccatccc gtttcaattg ttcaatattg attatttttg taaaaaaata
    13261 attgatggaa ctttacgaaa tattcaaaaa acatcctgta atatgtaccg atagccggaa
    13321 ttgtccggaa ggttcactgt tttttgcact gaaaggcgat aacttcgatg cgaatgcctt
    13381 tgcactttcg gcgctcgaaa agggttgcgc ttatgctgtt attgacgaca aacaatacgc
    13441 tgtggatgaa cggtttattc ttgtcaataa tgtactggaa accctgcagg atctggcgcg
    13501 tcaacaccgc cgtcaactgg gaactaccat tatcggaatt acaggcacca atggtaaaac
    13561 caccacaaag gaacttattg cagccacact caaacagaag tacaggattc atttcacaga
    13621 agggaatctg aacaatcata tcggggtacc tctgacactg cttcaactga cgccagaaca
    13681 cgaactggca gtcgtcgaaa tgggcgccaa tcatccgggt gaaataaaag tactggcaga
    13741 tattgcacag cctgattacg gcatcataac caacgtgggt aaagctcatc tcgaaggttt
    13801 cggctcattc gaaggagtca tgagaaccaa aggcgaactt tatgattaca tttataaaag
    13861 cgggggtaag atttttctga ataccggtaa tgcctttctg tgttccatgt cgaaaaaggc
    13921 aaaattcatc agcccggaca gaattctgtc gtatgaactc ggcaacaatc tgaaaggcca
    13981 tgtgtcagta ggtaaaaccc tatcgtgcaa cccgcttctc cacctgaaat gccagaataa
    14041 ggaaagtgaa ttcgaaatca ggactcaatt aattggaacg tacaacgccg aaaatgtatt
    14101 atcggctgct accatcggaa cacatttcgg agtgagcaat gaactgataa aaaaaggtct
    14161 ggaagagtat attccccgga ataaccgttc gcagttgact atcagcgaac acaataaaat
    14221 cattgctgat gcttataacg ccaacccgac gagtatgcac gctgccatcc tgaattttgc
    14281 gcagatggac gtacctgaaa aaaccctgat acttggcgat atgcttgaac ttggaaaaca
    14341 atcggccgat gaacatcaga aaattgcaga tctggtgaaa gataaagggt tttcttccgt
    14401 ttacctggta ggaaaaagtt ttcaggcgac caaaagcaat tttaaaaagt ttgccgacat
    14461 cggggcgctt atcgctgaaa tccgcgaaaa cccgctgcaa aacaaattta tactggtaaa
    14521 gggttcgcac ggcattcatc tggaaaaaat actggaattg atttaattac aaatacatat
    14581 ttgctaaaaa atggaaaaga aaaaaatgac catattactg gttgctttgg gtgttgtgat
    14641 tgcggccctg atagccggtt tgttatacac gatgaacgca tcgtcggaaa aagatgaaca
    14701 aattgcagca actgaagaaa tgatgacttc tgaaaaacaa caactggaaa ccgaatataa
    14761 agatctggca ggagaatacg acggatacac catgaccatc cacaacgatt ccctgctgaa
    14821 gcttttcaac gaagaaaaac taaaggtaaa taaactgctg gaggaactca aaaccactaa
    14881 aagctcgaat gccaaacgaa tcatagaact caaaaaagaa cttgcagccc tgcgcaaggt
    14941 gatggttcat tatgtcaatc agatagattc ccttaacctc caaaacaagc gccttaccaa
    15001 cgaaaactac gaagttcgtc agaaatatgc ctctgcatcg gctaccgccg agcaactttc
    15061 gaaagaaaaa gaaagtctga atgaagttgt aacacgggcc gccattctcg aagtgtataa
    15121 tttcagcatg acgccgctcg acaaacgcaa caaaaaaacg gaccgggcat ccaaaatcac
    15181 tacacttcag ttcaattaca ccatcggaaa aaacattacc gctactcccg ggcagaaaac
    15241 agtgtacgtg cgcctcaccc gccccgacga tgaactgatg acaaaaagta acgattccta
    15301 tttcacatac gaaaataaac aaatagcctg ctcgctcagc aaggactttg aatatagcgg
    15361 tgaagcacgc aacgacgttc tttactggaa agtagatgaa atcctgcaaa taggcaacta
    15421 ccgtgccgac ttctttgcgg acaacaacag aataggtaca tttaccttcc ggattgaaaa
    15481 aaattagtat ctttacagtc agtaaaatga gcatattccg atgactgcag agtcccgaaa
    15541 atttggcatt gctgccggaa aaatagcttt cgatgaaaag caccgaagca cgataaaatt
    15601 caacatttcg cgctacgagg ctgcctttgc caaaggtctg gcgcgctaca aaaacgtaaa
    15661 tagtgcaaaa agtactgctg ctgccatcaa acgggatgtt ctgaaaaact gggataaata
    15721 tctgctggaa tttgagaaaa acataacagc caacggtgcg gaggtgcttt gggctgaaaa
    15781 tgaaactgaa gcaacagcat acatccggaa aattctgacc gaaaacgaag cccgtctgct
    15841 ggtgaaaagc aaatcgatga ccaccgaaga gattgaactc aatgaaacag caaaaagtct
    15901 gggtgtcgaa tcggttgaaa ccgacctggg tgaattcatc gtgcaggttg caggcgaaaa
    15961 gccatatcac atcgttactc cggccatgca taagtcaaaa aaggacattg ccgtgctttt
    16021 caacgaaaaa ttcaataccc ctgccgacag cactcccgaa gagttgaccg attacgtaag
    16081 aggggttctg agaaaaaaat atacggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnngag gccgacggac ctgaaaaaat gtacgtaatt ttgctggata acgggagaac
    16261 aactatattt gcagatgatg aagcccgcga gtcgctggct tgcatccgtt gcggagcttg
    16321 tctcaatggc tgtcctgttt ataaaactat cggcggctac acgtacgaca gtacctacag
    16381 cggaccaatt ggctcagtga ttaccccgtt tctgaaagga tttaaagatt tctcgcatct
    16441 cagttttgca tccagtctct gcggtagatg caacgaagtt tgccctgtta gaattccgct
    16501 caccgacatc ctgcttgcaa accgcagaaa agcagtcgaa cagggattaa ggccaaaagc
    16561 cgaaaaatgg gtgatgaagg gttacaggta tatcagttcg aagcgtgcgg catttgattt
    16621 tttctctccg ggactgaaga atattggagt ggcgccgttc aattatttcg gctggggacc
    16681 tcatagaacg atgccttctt tttcaaaaaa atcattttcg gaaacataca gatataaata
    16741 taaaaactta attaaataat gaaaaccaat tttacaaaga atatactgat attgctggga
    16801 atttcagttt tttctttttt atttacttcc tgtgaaaaag aagataaata tattgactgg
    16861 aaagtactga atgaacgctg gcttgaacaa cataagaatg atcccggatt taaggtaaca
    16921 gaatcgggtc tatgctacaa agtgatccgg cagggcaacc cgaatgaccg taaagccaca
    16981 tcgacaagtt acatctatgt taactacgaa ggcaagctga taaacgactc cattttcgac
    17041 aaaaaagaac aatttcaaga ctttttgtca gaaatggttg acggatggaa ggaaggcatt
    17101 ataaaaatgc ataccggtga tatttatcag ttctatattc cctgggaact gggttatggt
    17161 aaagatggct tcggaagcat tccgccctat tccacgctta tattccaggt ggagttggtt
    17221 tactctgtga attaataaag acatcatgaa acgcgcaggt tttatactgc tttttggtct
    17281 gttgctggga gtaatttctg ctcaggagta taccgttgaa agtgttccca atccgattac
    17341 actcaatgcc catcattatg taagtaatcc ggacggcata ctcagtgaaa gtaccgtaaa
    17401 cgccctgaac gttaaactgg acagtctggc acaaaccaac ggcgctcagg ttgctgtggt
    17461 tgttctcaat tccattggct atgaagatat taacaacttc gcatggagcc tgttcgacaa
    17521 atggagaatt gggaaagaaa aagaggacaa cggactgctc gttctttttg tactggatca
    17581 gaaaaaagtc cgttttgaaa acggatacgg cctggagggt gtcatccccg atgctattgc
    17641 caaacgcatc cagaccacag ccatgatacc cgagttcagg aatggtaatt atgatgccgg
    17701 aatgatagcc ggtgtcaatt ccattatttc ggcaattaac cgtgaaccgg tggctgaaga
    17761 agctgtgaaa cctgcttttc aatgggatga agtactccct tatgcgctgg ctgcatatat
    17821 tctgttgtct ctgctcactt ttttgtggac ccgcagcgtc gtcaggcagg caaagaaaaa
    17881 caacacgctt accagcaatt tgtcgcgcta caaagtgatc agggaaaaca acaattctgt
    17941 cgcatcgatg atcggattat atctcccgct gatagccctg gccttgatac tttttttcgc
    18001 cagtgccgtt taccttattt tcctgcttcc tgtaccgctc accgtactgc cgtcgtacct
    18061 ttatggacga cgtcagatga ctaaggttcg gcgctcgccc atcccctgta aagtatgcgg
    18121 gaatcagatg catatcctgt cagaaaaaga agatgacgct catcttcggc tttcgcagca
    18181 gcttgaagaa gagctgaaag ctgtagatta cgatgtgttt gtgtgcgaaa gctgcaagga
    18241 aaaagcaatt tttacattag ataaaaaaag ccagtacgaa acttgtccgc gatgcagcac
    18301 caaagctttt attcttgatt cgaaaagaat gattgtggcc ccaacttatg ttcatgccgg
    18361 tacggaacga ttgacctata aatgcaaatt ctgcggatat caggaaaata acaaccgcaa
    18421 ccttccacgc ctgaaacgca caaacagcgg tttctttggt ggtgccgttg gtggaggcat
    18481 gttcagcggt ggcggtggtt ttggtggcgg cggaggaagc ttcggaggcg gcatgtcggg
    18541 cggaggtggc gctaccagtg gctggtaagg ttttattgta tttaaattgt atttaaaatg
    18601 aatctatgca ttattaatta taaattttaa ttttatgaag aagacaggta tcatcattat
    18661 tatcgcactc gcagctattc tttttttatg gggaatggga ggctacaacg gactggtaaa
    18721 ttctcaggaa aatgtcaaat cgcagtgggg gaatgtagaa aatcagtatc agcgccgtgc
    18781 tgacctgatc cctaacctgg tagaaaccgt gaaaggctat gctgcgcacg aaaaatcgac
    18841 attcgaagag gtaattgctg cacgtgccaa agccacacaa attacaatag atcccactaa
    18901 cctgacacct gaaaaacttc aggaatttca ggctgcacag ggtcagttgg gtagtgctct
    18961 gggtcgttta atcgctgttg ctgaaaatta tcccgatctg aaagccaatc agaattttct
    19021 ggaactacag gctcagctcg aaggtaccga aaaccgcatc tcggtggaac ggaacaaatt
    19081 caacgaaatg gccaaagaat acgatgtagc catccggcga ttccccaaaa atattcttgc
    19141 aggaatattc aattttgaaa atatccctta ttttaccgcc caggaaggtg cagaaaaagc
    19201 acctcaggta aaattctgat tttttattat tgtccgataa aaacaaaaaa aaataatgtc
    19261 gctcagatgg gagcttttgt tgatgggtgt aatctgattt ctaccataat gtcgcaccgc
    19321 tggtgctaaa aattaaagcc tcgtagaggc aaaattatgg tagaaataat atgtaacaga
    19381 atataaagcc ttatagaggc gacattatgg caaatcacgt tttataagga cttacaggat
    19441 ttttctaaaa tgtttaccgg acaacaataa ttaattatat agaaacataa actccaacct
    19501 gtcacgaaat ggcaggttgt ttaataatta aaatatgtca tacactccat cccccgacag
    19561 atatacaaat tctgtaggat acaactactg cggaaaaagc ggactgcaac tgccaagatt
    19621 atcgatggga ctttggcaca acttcggtga cgtaaatgac ctgaaggaag cccgaaaaat
    19681 gcttgaaacc gcgtttgata atggtatcac acattttgac ctggcaaata attatgggac
    19741 accctttgga agtgctgaag caaattttgg aaagatactg aaagaggatt ttgccccata
    19801 ccgcgacgaa cttataatta gcacgaaagc cggacacacc atgtgggacg gaccgtatgg
    19861 cgactggggt tcacgcaaat atctcattgc cagccttaat caaagcctga aacgtatggg
    19921 tcttgattat gtggacattt tctattcgca ccgctacgat ccacatactc cactcgaaga
    19981 aaccatggga gcactgtcgg atatggtcag acaggggaaa gcactttacg ttggcatatc
    20041 aaaatatcca actgataaag cacgtcaggc ataccgcata ctggccgata atggcacacc
    20101 ttgccttatc aatcaggaca ggtacaacat tctgacacgc gatattgaac aaactgtatt
    20161 gccgcttgcc aaggaaaaag gaataggttt tattgcattt tcacctttag cccagggctt
    20221 gctcagtaac aagtatctga aaggaatacc tgaaaattca cgcgcagctc aatcttatgg
    20281 ttttctacag aaagatcaga taacacccca acttgtaagt aaactcatta aactcaatga
    20341 attcgctaaa aaaagaggtc aaagtctggc tcagatggct ttagcctggt gtctgcatga
    20401 cagtcaggtt aattctgtta tcatcgggac aagctctgtg aaacagttga aagataacct
    20461 tggtgcactg aaaaacactc attttacgga tgaggaaatg tggctgatcg gggaaattgc
    20521 cggataagat tttatcgcat attcaaacat tcggcacggg ttaaacacag tccgtgtcga
    20581 ttgtatttta aaccggattg tcagtattca atcttatcct ctttatcttt ccatagttta
    20641 aaagcatctg tggcttcatc ggcgagtaaa tgatgagttt caactttacg ttgttcgggc
    20701 ttcagagcta tctcgtcgta aataaacgaa tcgtcaaagc cgatatctct ggcttctgct
    20761 tttgtgtttc catagtatat tttgtctaaa tgtgcccagt aaatggcgcc aaggcacatg
    20821 ggacaaggtt cacaggaggt atagattgtg caaccttcaa ggctgaagtt atgaagtttt
    20881 ttgctcgctt tgcgtattgc cagaatttcc gcgtgcgcag tcgggtcgtt gcttcctgta
    20941 acatgattga caccggtagc tataattttt ccctccttaa caatcacggc accgaaaggt
    21001 cctccaccct tttttacatt tctcactgac agcgctatgg ctttccgcat aaatttttcg
    21061 tgttcactca tatccatact caaagaatta tttttaactt tacaaaaata tcaatttaac
    21121 tgatatttcg tcttaattat tcagaattaa ttgattatgt taattatcag catttataag
    21181 acctatatca ttccggcttt tgatattttt gttgcatcgt tttacagaat tttcgtactt
    21241 tgcatctcaa aatcatcagc acaaatcata atacgttaat atta
//
LOCUS       DAOPYB010000031        21162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2558_length_21162_cov_10.446487, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000031 DAOPYB010000000
VERSION     DAOPYB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21162
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2558_length_21162_cov_10.446487"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..133)
                     /locus_tag="PKH58_04050"
     tRNA            complement(61..133)
                     /locus_tag="PKH58_04050"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:Gly,seq:tcc)
     gene            complement(217..299)
                     /locus_tag="PKH58_04055"
     tRNA            complement(217..299)
                     /locus_tag="PKH58_04055"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(263..265),aa:Tyr,seq:gta)
     gene            complement(380..453)
                     /locus_tag="PKH58_04060"
     tRNA            complement(380..453)
                     /locus_tag="PKH58_04060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(417..419),aa:Thr,seq:tgt)
     gene            complement(566..865)
                     /gene="raiA"
                     /locus_tag="PKH58_04065"
     CDS             complement(566..865)
                     /gene="raiA"
                     /locus_tag="PKH58_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNX88232.1"
                     /translation="MKVMIQSINFDATEKLEEYIIKKTKKVEKFFDEIRIVDIYLKVV
                     KPETVTNKQAEIKVTAPNVDFFASKTCDSFEEAVDLALEAIEKQVIKYKEKVSKK"
     gene            complement(862..1761)
                     /locus_tag="PKH58_04070"
     CDS             complement(862..1761)
                     /locus_tag="PKH58_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX88233.1"
                     /translation="MINDFIQYLQYEKNYSSHTVLSYHTDLIQFCDFLQIAPDDFNPK
                     EIQADIIRQWVLKLLDGKNTARSISRKISTLKSFWKFLQLHGLAEQNPTLKIVLPKTK
                     KTLPAFYKQNEMEQVMAETKVLHSYEQVRDHLIIRLLYECGLRVSELVSLVDQDFEVE
                     SKQLKVLGKRNKQRIVPMSNELSEKISEYKVLRNKELKRFDNSLFVLISGKSMYTKAV
                     YNIVHREMSKVSTLHKQSPHVLRHTFATSLLNNGADINAVKDLLGHSSLAATQVYTHV
                     TFSELNKIYKQAHPRANKKGGNL"
     gene            complement(1847..2038)
                     /gene="rpsU"
                     /locus_tag="PKH58_04075"
     CDS             complement(1847..2038)
                     /gene="rpsU"
                     /locus_tag="PKH58_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNX88234.1"
                     /translation="MIVVPVKEGENIEKALKKFKRKFEKTGVVKELRRRQCFDKPSVV
                     KRERKMHAVYVQQIQRTEE"
     gene            complement(2161..3144)
                     /locus_tag="PKH58_04080"
     CDS             complement(2161..3144)
                     /locus_tag="PKH58_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88235.1"
                     /translation="MPTLKPLDWVSPRELYKSTVSCFLKTITKSDNYLLGHVAVKLET
                     PLLDKPLLTAQTSCGMKEKFSLVFKQKVGLGMLGLAIQGRMEPSEELSRKLKIYAERE
                     QLTFIRYKINEKAADRLIKFVKLYAMIRDNSLAPCNYYGGAFWPRYQDEGAGCSSFGI
                     AMMELAGIKPVDPEKWIVNVKIPMKLVGGHLNNGKKVQVKEIMKTNSWYESNGTPDID
                     FISYNVYEPSLMFEWVENKIKQKSLGYEIIEEDGVAGLLIDATNAVIDENEPLFLKNK
                     NPNLFINYHIQKIQNKIQLDTILNITQSLKIPEYLFLPAKIKNVDPSKIVF"
     gene            complement(3372..4073)
                     /locus_tag="PKH58_04085"
     CDS             complement(3372..4073)
                     /locus_tag="PKH58_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNX88236.1"
                     /translation="MQNKKKLVVLTGAGMSVESGLSTFRGAGGMWGQHRVEDVATIDA
                     WYRDKQKVLDFYNIRRKELLGVKPNIGHMVLAELEKDFDVKIITQNVDNLHEQAGSTH
                     VLHLHGELMKVRSCGPGAEVYDLSSENYETRLGDTCPKGYQLRPHIVWFGEAVPEMNT
                     AIDIVEKADIFLIIGTSLQVYPAASLLHYVPAKVPVYLIDPNEVSTKSRQVYVINKGA
                     GEGMLELKEKLMKYL"
     gene            complement(4082..4543)
                     /gene="queF"
                     /locus_tag="PKH58_04090"
     CDS             complement(4082..4543)
                     /gene="queF"
                     /locus_tag="PKH58_04090"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNX88237.1"
                     /translation="MNNQKLTLLGKKTEYKNEYAPEVLEAFDNKHPNNDYWVTFECPE
                     FTSLCPITGQPDFATIRINYIPDIKMVESKGLKLYLFSFRNHGAFHEDCVNIIMNDLI
                     ELMSPKYIEITGIFTPRGGISIYPYANYGRKGTKYEQLAEQRLFNHSFPVK"
     gene            4683..5531
                     /locus_tag="PKH58_04095"
     CDS             4683..5531
                     /locus_tag="PKH58_04095"
                     /inference="COORDINATES: protein motif:HMM:NF023205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3316 domain-containing protein"
                     /protein_id="HNX88238.1"
                     /translation="MKKAFILHLFIFSFLTMQAQDFTNERFPLTTSVKTMDIGNMNIL
                     DPYLSPYEYSGFQIGLFEENRRLMPSIDTLISYTSRKSLSFGSGNHPSKNNSMLMFNG
                     NYNFGLNYHIRPHKNFLVLVGGSWDIDLGGKYISRNVNNPFSLDLYTNLNATAELFYR
                     FKLKNQSLQLHYGFQTPVAGSMFVPMQGASYYELFTLSNMSNAIHFSSLHNKRGILQN
                     FQLDLPLKIATIRLGIQHELMQYSANEMIFHKKSLTFSVGYRVDMYTFTGRKNKVPSN
                     FISAYE"
     gene            5559..6569
                     /locus_tag="PKH58_04100"
     CDS             5559..6569
                     /locus_tag="PKH58_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX88239.1"
                     /translation="MKQIYVIISLFLMVELQACMDEPDLRSDTPRGNFNALWEIIDTR
                     YCYLSYKQINWDSVYTEYSHKIDTVSSNFSLFDVMGKMLATLKDGHVNLYSSFDRSRY
                     WNWYTDYPSNFNSGVLFKPHYLGADYRIAGSLRYKKIANGKVGYIYYGSFSDSFSNEN
                     INQIFNSFSSCLGIIVDVRNNGGGMLSYAEQLASYFFDKKTLTGYLSHKTGTGHSDFS
                     TPTPLYIEPNDKVKWNKKVMILTNRMSYSATNGFVNIMKYAPNSTIIGDQTGGGGGLP
                     FSSELPNGWMVRFSSSPMYDADMQNIEWGIKPDIQVNMNAEAEARGYDTIIERAIGEI
                     FK"
     gene            6612..7142
                     /locus_tag="PKH58_04105"
     CDS             6612..7142
                     /locus_tag="PKH58_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX88240.1"
                     /translation="MRKLKITELNRLTSEEFKSQQKTPLIVVLDNVRSLHNVGSVFRT
                     SDAYLVEAVYLCGITSPPPHAEIHKTALGAENTVFWKYYEETLGAINELKEKGYEVLA
                     IEQAEGSIMLNTLEINPAKKYAVVLGNEVKGVQQAAIDSCDGCIEIPQYGTKHSLNVS
                     VTAGIIIWEFALKLKK"
     gene            7207..7941
                     /locus_tag="PKH58_04110"
     CDS             7207..7941
                     /locus_tag="PKH58_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX88241.1"
                     /translation="MSYGQSYQKEIYKAYVDYEVNRWLKAAKYVENSNQNQTEKMLNL
                     IHCYYGYTSSLMSLKQNDEAEKIIEKAEILIDKILKTDPDNALALNYKGVFLGYEIGL
                     NKMKALKYGTKSTSYIDNALKLAPNNVQILFDKGNALYYPPKVFGGNKNEALKYFQKA
                     IGIIEKQKKTNENWVYLQLLALEGRCYELNGNYTKAEHSYQKALKAEPEFKFVKNTLY
                     PRLRKIMSNASTNQKQDDSPENEIYR"
     gene            7948..8292
                     /locus_tag="PKH58_04115"
     CDS             7948..8292
                     /locus_tag="PKH58_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNX88242.1"
                     /translation="MKKNDWKDRLNVVYSTNPDYKYESEEANKSEIATPTAQTLRIEL
                     DKRNGKPATLITDFQGSDNELKELAKTLKNKCATGGSQRDGEILIQGDFRVKIAEILQ
                     NMGYKIKKINFK"
     gene            8506..10008
                     /locus_tag="PKH58_04120"
     CDS             8506..10008
                     /locus_tag="PKH58_04120"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNX88243.1"
                     /translation="MNNLIKLNPNPLVSHLEKLPKDFTKADILKFIEEKKIKMINFRY
                     AGGDGRLKVLNFVINNYNYLDQILTAGERVDGSSLFSFIEAASSDLYVVPRFSTAYLD
                     PFSEIPTLGFLCSYFNKDGIPLESSPEYILKKAHQIFLQQTDGMEFYAMGELEYYVIG
                     KEEDAFPAVDQKGYHEAAPFSKFEDFRCKTMMYIAQVGGQIKYGHNEVGNFVHEGKIY
                     EQNEIEFLPSPVTDAADQLLLGKWIINKLAYQYGLDVTFAPKITVGKAGSGMHIHTCI
                     MKNGENQYVDKGKLTDTAKKAIAGFMTCAGSLTAFGNTNPTSYFRLVPHQEAPTTVCW
                     GDRNRSALVRVPLGWSSGTDMCANANPNEKTEISDFSVKQTIEFRAPDGSANLYLLLA
                     GLVTAAGHGFELENAVEFADKKYVDVDIHKSGNEAKTNGFEHLPTSCFESAELLDKNR
                     ETYIKHGVFSEAAINNIINQLKSFNDKKIREEIAAQPEMLQNLVDKYFYC"
     gene            complement(10094..11353)
                     /locus_tag="PKH58_04125"
     CDS             complement(10094..11353)
                     /locus_tag="PKH58_04125"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNX88244.1"
                     /translation="MQTIDKFNFAGKKAFVRVDFNVPVDENFNITDDTRIRAAMPTLK
                     KIIADGGRLIIASHMGRPKKNPDPKYSLKVIVNRVSELLEVPVKFAADCMGEETEKMV
                     AELKDGEALLLENLRFYAEEEGKPRLPETATEDEKAAAKKEVKASQKEFTKKLASYAD
                     CYVNDAFGTAHRAHASTALIADYFDADNKMFGFLMNKEVTAVDKLMDNPARPFTAIMG
                     GSKVSTKIEIIENLLTKVDNLIIAGGMTYTFTKALGGKIGISLCEDDKLELALELIEK
                     AKQNNVNLVLAVDAKIADAFSNDANTGFTPVGEIPDGWEGLDIGPETEKIFADVIKSS
                     KTILWNGPTGVFEFENFTHGSRAVGEAIVEATKNGAFSLVGGGDSVACVNKFGIADKM
                     SYVSTGGGALLEAIEGLVLPGIAAIKG"
     gene            complement(11362..11589)
                     /locus_tag="PKH58_04130"
     CDS             complement(11362..11589)
                     /locus_tag="PKH58_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88245.1"
                     /translation="MLKVSAISCGSLVAIAGINFLILIFRTKQTLNHDGLWIVGIGAL
                     FVMGLILILLISGIQSPILINQKKKEYITLK"
     gene            complement(11716..12222)
                     /gene="purE"
                     /locus_tag="PKH58_04135"
     CDS             complement(11716..12222)
                     /gene="purE"
                     /locus_tag="PKH58_04135"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNX88246.1"
                     /translation="MNAKVSIIMGSTSDLPVMDKTAKVLDEFEIPFEMQALSAHRTPA
                     EVEIFAKGACDRGIQVIIAAAGMAAHLPGVIASMTTVPVIGVPVNSSLEGLDALLAIV
                     QMPPGIPVATVGINGAMNAGILAVQILSVGNETLQRKLIDYKEDLKNKIVKANDELSK
                     IEYKFKVN"
     gene            complement(12281..12658)
                     /gene="gcvH"
                     /locus_tag="PKH58_04140"
     CDS             complement(12281..12658)
                     /gene="gcvH"
                     /locus_tag="PKH58_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNX88247.1"
                     /translation="MNVPANLKYTKEHEWVRVEGEFAFVGITDFAQSELGEIIFVEVE
                     TVGETIDQGEVFGSVEAVKTVSDLFLPISGEILEFNANLEDAPELVNNDPYGEGWIVK
                     ISCSNVAEVDGLLSADEYNALIG"
     gene            complement(12750..13880)
                     /locus_tag="PKH58_04145"
     CDS             complement(12750..13880)
                     /locus_tag="PKH58_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX88248.1"
                     /translation="MKKNSIKGRPMTAFIKWIMVLVNITAVLALGLVKIGSLISPEKL
                     LLPAYASLFLLPLIIINVFFIVFWIFCRKWYLLISLIAIIIFYGTVKSAFPVNLAKPD
                     IDTVSTKIKVLSYNTMNTGMMKKHKKNDPNPVIKYILDTDADVVCLQECAISENSYQF
                     NYDDFERIFKIYPYKHVSFQLNKWSMGIGLVTLSKYPIIEKRNAGYKSIFNLTIYTDI
                     IVGEDTLRIVNNHLESNRITAQDIQMTSKLKDGFDTDELGSVTKYLSQKLSVAYRVRA
                     QQADVVAKLIRESPYKVISCGDYNDVPSSYAYTKVLGNLKDSFKETGNGLGWTFEHSF
                     YRFRIDYIMYDKAFQSANYKRGNLKASDHYPIQTDLYLLKNR"
     gene            complement(13867..14742)
                     /locus_tag="PKH58_04150"
     CDS             complement(13867..14742)
                     /locus_tag="PKH58_04150"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX88249.1"
                     /translation="MNIFDNLRLTYKNGNTLIKLIYLNVAVFLLINIFLVIFRLFNID
                     ASEYINWLAIPSNLNKLLSHFWTPLTYMFLHKGFYHILFNMLMLFWFGRLFLTYFSEK
                     QLLSLYILGGLIAASVYVIAYNVFPYYSAAAYYSVLLGASGSIMAIVVATAVRAPNAE
                     MNLLLIGRVKLIYIAIITVLISVFSLTGNNGGGEMAHLGGALTGYLFAVTYKKGTDIT
                     YFITRIVDFFVNLFRPRPKQKAPKFHSQRMSPQEYNQQKARNEAEIDRILDKIKTSGY
                     ESLSADEKRKLFEKK"
     gene            complement(14796..15569)
                     /locus_tag="PKH58_04155"
     CDS             complement(14796..15569)
                     /locus_tag="PKH58_04155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX88250.1"
                     /translation="MNNYRSSFFSNIPPIVKNILAINVVLFVATILLPGILERRGISI
                     DLNDVMGMHYFASQKFNPAQMITYMFMHGSFSHIFFNMFAVYMFGGILERVWGPKKFL
                     IFYLVSGLGAAIVQQLFWYFEFHTVVSAFNSAISSGSVDSLRAYFSGDISGMTVLDVI
                     KQKDLLLNMPVTIGASGSVFGLLLAFGWLFPEERLFLMFIPVPIKARWFVLGYGVAEL
                     FLGVAQFSGDSVAHFAHLGGMLFGALLIYYWKKKGRLYS"
     gene            15825..16097
                     /locus_tag="PKH58_04160"
     CDS             15825..16097
                     /locus_tag="PKH58_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX88251.1"
                     /translation="MNKAELINAIAEASGMTKVASKKALDATIKSITGSLAKGEKVSL
                     VGFGTFSVAERAARTGINPSTKKSLKIPAKKVAKFKAGADLADAVK"
     gene            16266..18059
                     /gene="argS"
                     /locus_tag="PKH58_04165"
     CDS             16266..18059
                     /gene="argS"
                     /locus_tag="PKH58_04165"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNX88252.1"
                     /translation="MSLESTLTGGVIQAISELYGQTITPDIIQFQKTKKEFEGDITLV
                     VFPFVKLARKSPEQTGEEIGAWLKSRFEIIEKYNIIKGFVNFSIDTSYWIGQLAEIHQ
                     LPEFGTTEPQDDAALMMIEYSSPNTNKPLHLGHIRNNLLGFSIAEIQKANGWKVVKTN
                     IVNDRGIHICKSMLAWKLFGNGETPERSAMKGDHLVGKYYVEFDKAYKAEIRELMEKG
                     LSEEDAKKEAPLIKQAQDMLLKWEQGDEEVRSLWQMMNGWVYAGFDETYRKMGVDFDK
                     IYYESETYTEGKSKVEEGLQKGVFYKREDGSVWANLIDDGLDEKLLLRADGTSVYMTQ
                     DIGTAKLRFDDYPINKMVYVVGNEQNYHFQVLSILLDKLGFEWGKGLVHFSYGMVELP
                     EGKMKSREGTVVDADDLIEEMVTTARETSQELGKLDGVSEEEAENIARMVGLGALKYF
                     ILKVDPKKNMTFNPKESIDFNGNTGPFIQYTHARIKSVLRKAEDLGINYNEALNVQTV
                     LSEKEKYLIQLLTEFPATVKQAGDEFSPALIANYIYELVKEFNQFYHDYSILKEENKD
                     LRNFRLVLSASIGNVIKTGMGLLGIEVPDRM"
     gene            complement(18176..19099)
                     /gene="prmC"
                     /locus_tag="PKH58_04170"
     CDS             complement(18176..19099)
                     /gene="prmC"
                     /locus_tag="PKH58_04170"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNX88253.1"
                     /translation="MQHFLKKLNTSLADMYSEAELHMIANLLLENITGLSRVQLLSNK
                     DLILNDEKNILADRYLLQLKNHEPIQYILGETEFYGLKFKVNPSVLIPRPETEELVEW
                     VSPHPLRYFPHKGEGMEEIGVFKSPRGDLGGFFLDIGTGSGCIAVSLKKKFPFANVAA
                     MDISSEALEVARENAKINHLDIEFIQDDILNPAPTDRKWDLIVSNPPYIPASEHSEMD
                     KNVTDFEPHLALFVHDNDPLIFYRKIAEFGITHLSPGGKIFFETHQNLAHECKLLLEA
                     HGFINVEVRKDISGNDRIVRASKKNDKSDLL"
     gene            19291..20814
                     /locus_tag="PKH58_04175"
     CDS             19291..20814
                     /locus_tag="PKH58_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNX88254.1"
                     /translation="MNFRKIFTALLLIIISTGLTAQKYQKREMRAVWIATVANIDWPS
                     KSGLTPTQQRIEMRKMLDDLQECNINTIVLQIRPTADSFYPSELEPWSNYLTGKQGQR
                     PNPFYDPLQFIIEEAHKRCMEVHGWVNPYRVTNAAGQINRLDKSHIYYKSKDLIVKYG
                     DKYYFNPGLDETREFLNKVVEDIVEHYDIDGLHFDDYFYPYPEGGRDFPDEETFRANP
                     RGFTNKADWRRNNVNMVIEELQKTIKDMKPWVQFGVSPFGVWRNDNKDAQGSATRAGI
                     TNYDDLYADILKWLREGTIDYVVPQLYWEIGKKVADYEVLVKWWSDHSYGKNLYIGLY
                     ASGLIENKTEAWKKGNELARQLKMNRNFPKTEGAVYFSAKALLKNPRGLVDTLKNNYY
                     KFPALQPICHNIKGEASAQPQNIRVVKDGKEAYILWDEVVEDGGCQVAYYVVYGFKGK
                     KIGDMNDPENIVAITTENCIDLNAYEKKFKGRCSFVVTAVNRFRHESIPTQGVTRRM"
     gene            complement(20963..>21162)
                     /locus_tag="PKH58_04180"
     CDS             complement(20963..>21162)
                     /locus_tag="PKH58_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88255.1"
                     /translation="FNVKSDNRTKAQLVAQSMLPENPDDLIGTVCPVCGKGKIIKGKT
                     AYGCSEWKNGCTFRKNFVTAP"
BASE COUNT         6670 a         4260 c         4042 g         6190 t
ORIGIN      
        1 aacccatgac ctcttcctta ccaaggaagt gctctaccac tgagctacaa cagcaaaaag
       61 agcggaagac ggggctcaaa cccgcgaccc tcagcttgga aggctaatgc tctatcgact
      121 gagctacttc cgcaagttaa atttgaaaat tggctgattt gaagatttga aaatgtatct
      181 tcaaattttc aaatttctaa attttcaaat ctgttgtggg cagtgatgga ttcgaaccac
      241 cgaagtcgaa agacagctga gttacagtca gccccatttg gccactctgg taactgccca
      301 ttgttcaaaa tcatttgttc atatcacaca ttaaaaagcg ctcttttaat gtcctttttg
      361 atatgctgtt tgctttttga gcctcttgtc ggattcgaac caacgacccc gagattacaa
      421 atcacgtgct ctggccaact gagctaaaga ggcaaaatcc attataatag cttttgaggg
      481 ctaaaaagcc gttcaaatat cgctattcaa acggcttgca aaaatacaaa attattttga
      541 tatacaaaat atatttacca aaattttatt ttttagaaac tttttccttg tattttatca
      601 cttgtttttc gattgcttct aaagccaaat ctacggcttc ttcaaatgaa tcgcaggttt
      661 tggatgcaaa aaaatctaca ttgggagccg taacttttat ttcagcctgt ttgttggtta
      721 cggtctccgg ctttacaact ttcaggtaga tatcaacaat tctgatttca tcaaagaatt
      781 tttctacttt ttttgtcttt ttaatgatgt attcttccaa tttttctgtg gcatcaaaat
      841 tgatggattg aatcatgacc ttcataagtt acctcctttt ttattcgccc ttggatgggc
      901 ttgtttataa attttattta actcgctgaa tgtaacgtgg gtatatacct gagttgcagc
      961 aagactgcta tgcccgagta aatctttaac cgcattaata tcggctccat tgtttagtaa
     1021 tgaagtagca aaggtgtgac gcagtacatg cggactttgc ttgtgaaggg tacttacctt
     1081 actcatttct ctgtgtacaa tattatacac agcctttgta tacatcgact tgccagagat
     1141 aagcacaaaa agcgaattgt caaaccgttt aagttcttta tttctcaaaa ctttgtattc
     1201 tgatattttt tcacttaatt cattgctcat cggaactatc ctctgcttat ttcttttacc
     1261 caaaacctta agttgctttg attctacctc aaaatcctga tcgaccagtg aaacaagttc
     1321 ggagacgcgt aatccgcatt catacaataa tcggataatc aaatgatcgc gaacctgttc
     1381 ataactatgc aacaccttag tttcagccat aacctgctcc atctcatttt gtttatagaa
     1441 cgcaggaagt gtcttttttg tttttggtaa aacaattttt aaagtgggat tttgctctgc
     1501 cagtccatgc aattgcaaaa acttccagaa ggatttgagg gtagatattt tacgtgaaat
     1561 tgatcttgca gtatttttac catcgagcag tttaagcacc cattgacgta taatatcagc
     1621 ttgaatttct ttgggattaa aatcatcagg agcaatttgt agaaaatcac agaactgaat
     1681 gaggtcggta tgatatgaca aaacagtatg agatgaataa ttcttctcat actgtaaata
     1741 ttgaataaaa tcattgatca tacctgacaa attcgttgta aaaattcaac aacgaagtta
     1801 tataattatt gtcagaatgc caagttttca gtaaaaatat ttttacttat tcttctgttc
     1861 tttgaatttg ttgtacgtaa acggcgtgca ttttcctttc acgctttacg actgaaggtt
     1921 tatcgaagca ttgacgacga cgcaattcct ttaccacacc ggttttttca aatttacgtt
     1981 tgaatttttt cagagctttt tcaatgtttt cgccttcttt tacaggtact acaatcataa
     2041 taatttctta tttaatatgt tatatttgtt atttcgaaat aggactgcaa agatagagaa
     2101 tcatttttaa atagcaaaaa gaaaaatagt tttttattac ttagtttttc ttcgattttt
     2161 tcaaaaaact atctttgaag gatcaacatt tttaattttt gctggcaaaa acaaatattc
     2221 gggtattttc aggctctgag taatatttaa tattgtgtcc aactgaattt tattctgaat
     2281 cttctgaata tgatagttga taaacagatt ggggtttttg tttttcaaaa acaagggttc
     2341 gttttcatct atcacggcat ttgtagcatc aatcagtaag cctgcaacac catcttcctc
     2401 aataatttca tatcctaaag acttctgttt tatcttattt tctacccatt cgaacatcaa
     2461 actgggttcg tacacattat agcttataaa atcaatgtct ggagttccgt tactctcata
     2521 ccagctattg gtcttcatta tttcctttac ttgtaccttc ttgccgttat tcaggtgccc
     2581 cccaaccaac ttcatcggta tttttacatt cacaatccat ttttctgggt caaccggttt
     2641 gatacctgca agctccatca ttgcaattcc aaacgaggaa cacccggcac cttcatcctg
     2701 atatcgcggc caaaatgctc caccataata attacaaggt gccaatgaat tatctcttat
     2761 catggcgtac agtttgacaa acttaatcaa tctgtcggct gctttttcat tgattttgta
     2821 acggataaat gtaagttgtt ctctttcagc gtatattttt agtttgcggc tgagttcttc
     2881 cgaaggttcc atacgacctt gaatagccaa accaagcatg ccaagtccta ctttctgctt
     2941 aaaaacaaga ctgaacttct ctttcatgcc acatgaagtt tgagcggtta gtaatggttt
     3001 gtccagcaga ggcgtctcca acttcacagc aacgtgacct aacagataat tatctgattt
     3061 tgtgatggtt ttcaaaaagc aactaactgt acttttatat aactcacgcg gactgaccca
     3121 gtcgagcggt ttcagggtgg gcataatgta tatagtgagt tcatgaccct gattaccccc
     3181 ggagtacgca gataaagggc tgaaaaaagc gaacaataac aaatgcaaac aaacaaacaa
     3241 tgtttttcta atgttatatt tcataatata atggaaacaa taacctatcg aaactaatat
     3301 ttttttgtaa agatatgaaa tcaaatgtga attccaaatg atttagcttt caaattaagt
     3361 tttcactata atcaaagata tttcatgagt ttctctttca attccagcat accttcaccg
     3421 gcacccttgt taataacgta aacttgcctg ctttttgtac tcacttcatt cggatcaatc
     3481 aaatagacag gcactttagc aggaacatag tgcagcagac ttgcagccgg ataaacttgc
     3541 agggacgtgc cgataatcag aaaaatatcc gccttttcaa caatatcaat tgcagtattc
     3601 atttccggaa cagcttcacc aaaccacaca atatgaggac gcagctgata tcctttggga
     3661 caggtatcgc ctaacctcgt ttcataattt tcagatgaaa gatcataaac ctctgcaccc
     3721 ggtccgcatg atcgcacctt catcaattca ccgtgcagat gtaaaacgtg agtgctgccg
     3781 gcttgctcat gaagattatc aacattttga gtgatgattt ttacgtcaaa atctttttcg
     3841 agttcagcca acaccatatg tccaatattt ggttttacac ccaataattc tttgcgacgg
     3901 atattgtaga aatccagaac tttctgtttg tcgcgatacc aggcgtctat agttgccaca
     3961 tcctccaccc ggtgctgccc ccacatacca ccggctccac ggaatgtact gagtccactt
     4021 tcaacactca ttccggctcc ggtcaaaacg accaatttct ttttattctg catgacttta
     4081 tttatttcac cggaaatgaa tgattaaata atctttgttc agccaactgt tcatatttag
     4141 ttcctttacg cccataatta gcataaggat aaatactaat gccaccgcga ggagtaaaaa
     4201 tccctgtaat ttctatatat tttggcgaca taagttcgat aagatcattc ataatgatat
     4261 ttacacaatc ttcatgaaaa gcaccatgat tcctaaaact aaacaaatac aacttaaggc
     4321 ctttactttc gaccatttta atatccggta tataattaat acggattgtt gcaaaatcgg
     4381 gttgtcctgt aatgggacac aaactcgtaa attccggaca ttcaaaagtt acccaataat
     4441 cgttattcgg gtgtttattg tcaaacgctt ccaaaacctc aggagcatat tcatttttat
     4501 attcggtttt ttttcctaat aaagttagtt tttggttgtt catcattgta tattttatta
     4561 gaagttcata ctaaatttta ttccgtaaaa ttacaaatct ttcagtactt ttttaaaaaa
     4621 acacaactga cttctttatt ttttatattt ttgtactaat tcaaacgtaa acagcatatc
     4681 agatgaaaaa agcatttatt ctacatctct tcattttctc ctttctgact atgcaggccc
     4741 aggatttcac caatgaacga tttccgctga ccacctctgt gaaaaccatg gatattggaa
     4801 acatgaatat tctcgatcca tatttatcgc catatgaata cagtggattt cagatagggc
     4861 ttttcgaaga aaatcgacgt ttaatgccgt ctatcgatac tctgatttcg tacaccagca
     4921 gaaaatcgct ctcattcgga tcgggtaatc atcccagcaa aaacaattcg atgctgatgt
     4981 tcaacggcaa ttataatttt ggattaaact accatatacg tccacataaa aattttctgg
     5041 tattagttgg tggttcgtgg gacattgatc ttggtggaaa atacatcagc cgcaatgtca
     5101 ataatccttt ttcgctcgat ctttacacaa atctgaatgc aacagctgaa ctgttttaca
     5161 gattcaagtt aaaaaatcag agcctgcaac tgcattatgg atttcaaaca ccggtggcag
     5221 gatctatgtt tgtgcctatg cagggagctt cgtattacga actttttacg ctgagcaata
     5281 tgagcaacgc tattcatttt agcagcttac acaacaaaag aggcatattg caaaatttcc
     5341 aactcgattt accgttgaag attgcaacta tccgactggg aattcaacat gaattgatgc
     5401 aatattcggc aaatgaaatg atatttcata aaaaaagtct aacattctct gttggttatc
     5461 gggttgacat gtatacattt actggaagga agaataaagt gccttcaaat ttcatcagcg
     5521 catatgaata accataaagt gtaacaacga atttacttat gaaacaaata tacgttatca
     5581 tatcgctatt tttgatggtg gaacttcagg catgtatgga tgaacctgat cttcggtcgg
     5641 acactccacg tggaaatttc aacgcgctct gggagatcat tgatacacga tattgttacc
     5701 tgagctataa gcaaataaat tgggattcag tttacacgga atattcacac aaaatcgata
     5761 ctgtttcaag taatttctct ctctttgatg tgatgggcaa aatgctggca acactgaagg
     5821 atggacatgt caatctctac tccagtttcg acagaagcag atactggaac tggtacacgg
     5881 attatccgtc aaatttcaat tctggtgttt tgttcaaacc tcattattta ggtgctgatt
     5941 accggatagc cggaagttta cgctataaaa aaatagcaaa cggaaaagtt ggttacattt
     6001 actacggaag tttttcggac agcttttcga atgagaatat aaaccagatc ttcaatagct
     6061 tctcatcatg tctgggaata atcgttgatg tacgaaacaa tggaggcgga atgctaagct
     6121 atgctgaaca gcttgcctct tactttttcg ataaaaaaac actcacaggc tatcttagtc
     6181 acaaaacggg gacaggtcac tcggactttt caactccaac tccgctttat attgagccca
     6241 acgacaaggt caaatggaat aaaaaagtaa tgatattgac aaaccgaatg tcgtacagcg
     6301 ccaccaatgg ttttgtcaac attatgaaat atgctcccaa ctccactatc atcggcgacc
     6361 agacaggagg tggaggcgga cttccatttt cgagtgagct gcccaacgga tggatggtcc
     6421 gattctcaag cagcccgatg tacgatgccg atatgcagaa tattgaatgg ggaattaaac
     6481 ctgatattca ggtaaatatg aatgccgaag cagaggcaag aggatatgac accatcatcg
     6541 aaagagctat cggtgaaata tttaagtaga ttttattaat tttgcttcga ttattatctg
     6601 actgcaaaca tatgcgaaaa ctaaaaataa cagaactcaa ccgactgacc tcagaagaat
     6661 tcaaaagcca acagaaaacg ccactaattg tagtgctgga taacgtaaga agtcttcaca
     6721 atgtaggctc tgttttcaga acttcagatg cataccttgt tgaagcagtg tatctttgtg
     6781 gcatcacctc tccccctccc catgctgaga tacataaaac cgctttggga gctgaaaaca
     6841 cagtattctg gaaatactat gaagaaactt tgggcgccat taatgagctg aaagaaaagg
     6901 ggtatgaagt attagccata gaacaggctg aaggaagtat tatgctcaat acactggaaa
     6961 taaatcctgc aaaaaaatat gccgttgttt tgggtaatga ggtcaaaggc gtacagcaag
     7021 cagcgattga ctcctgtgat ggttgcatcg aaatacctca gtacggtaca aaacactctc
     7081 ttaatgtgag tgttacagca ggaataatta tttgggaatt tgctttgaaa ttaaagaaat
     7141 aataaagttg tatctatgtc atttaggaga atttatacat taatcttagg gttacagttg
     7201 tgtcttttga gttacggtca atcctatcaa aaggaaattt ataaagctta tgttgattat
     7261 gaagtcaaca gatggctgaa agcagctaaa tatgttgaaa actcgaatca gaaccagaca
     7321 gagaaaatgt tgaacctgat acattgctat tatggttaca cctcaagcct tatgtcgctt
     7381 aaacaaaatg atgaagctga aaaaattatt gaaaaagctg aaattttgat agataaaatt
     7441 ctgaaaacag atcccgacaa tgcacttgcg ttaaactata agggtgtttt tcttggttat
     7501 gaaattggac tgaacaagat gaaagcttta aagtacggta caaaaagcac cagctatatt
     7561 gacaatgctc tgaaacttgc tcccaataat gtacaaatat tgtttgataa aggcaatgct
     7621 ctctattatc cgcccaaggt ttttggcggc aacaagaacg aagccctgaa atacttccag
     7681 aaagccatcg gaataatcga gaaacaaaaa aagaccaatg aaaactgggt atatcttcag
     7741 ttacttgctc tggaaggacg atgctacgaa ctgaacggca actacacaaa ggcggagcat
     7801 agttatcaga aagcgttgaa agcagaacct gaatttaaat ttgtaaaaaa caccctttat
     7861 ccccggttaa gaaaaataat gagcaatgct tcaaccaatc agaagcagga cgactctccc
     7921 gaaaacgaaa tttacagata gataataatg aaaaaaaacg actggaaaga ccggctgaat
     7981 gtggtatatt ccaccaatcc ggactataaa tacgaatcgg aggaggcaaa taaatctgaa
     8041 atcgccacac caactgctca aacgcttcgt atcgaacttg acaagcgcaa cggcaaacca
     8101 gctacgctga ttaccgactt ccaggggagt gacaatgagc tgaaggaact tgccaaaacg
     8161 ctgaaaaaca agtgtgccac cggcggttcg caacgcgacg gtgagatact cattcaggga
     8221 gacttcagag tgaagatagc cgaaatactg caaaatatgg gttataaaat taaaaaaata
     8281 aatttcaaat aaaagttaaa aacaccgcag gcatagattg aaaaaactaa atagtttcaa
     8341 aaaacacata taacacattg tccaaaatgt cggccaatgt gttttttttg taaagtcagt
     8401 ttgaaaatgt gtcattttgt cattatttta tacacagata tgctatttta aagaagtaaa
     8461 atatgtatat ttgcgtattt ctccataatt taatatcaca tttccatgaa caacttaatt
     8521 aaacttaatc caaatccctt agtttcacac cttgagaaac ttccgaaaga ctttacaaaa
     8581 gccgacattc tcaaatttat tgaagagaaa aaaataaaaa tgataaactt tcggtatgcg
     8641 ggaggtgacg ggcgactcaa agtactgaat ttcgttatta ataattataa ctatctggat
     8701 caaattctga ccgctggcga acgcgtagat ggatcaagtc ttttttcatt tattgaagca
     8761 gcttcgtccg atttatatgt agttccccgt tttagtaccg cttatctcga tcctttttct
     8821 gaaattccaa cacttggttt tctttgttca tacttcaaca aggatggtat accgcttgaa
     8881 agttcacccg aatatattct gaaaaaagca catcagattt ttctgcaaca aactgacgga
     8941 atggagtttt acgcgatggg cgaactggaa tattatgtca ttggcaaaga agaagacgca
     9001 tttcctgccg ttgaccagaa aggatatcat gaggcagcgc ctttcagtaa gtttgaagat
     9061 ttcagatgca agacaatgat gtacatcgca caggttggag gacaaattaa atacggtcat
     9121 aatgaggtcg gcaattttgt tcatgaagga aaaatttatg aacagaatga aattgaattt
     9181 cttccatcgc ctgtcactga tgccgcggac cagctgcttt tgggtaaatg gataattaat
     9241 aaactggcat accaatatgg tcttgatgta acatttgcgc ccaagattac ggtagggaaa
     9301 gccggaagcg gtatgcatat ccatacctgc attatgaaaa acggtgaaaa ccaatacgtt
     9361 gataaaggta aactgacaga taccgcgaaa aaagccattg caggttttat gacttgcgcc
     9421 ggttcgctca ctgcttttgg taacaccaat ccgacatcat atttccggct ggttcctcat
     9481 caggaggctc ccactacggt atgctggggc gatcgaaacc gttctgcact tgtgcgtgtt
     9541 ccgcttggct ggagttccgg aacagatatg tgcgccaatg caaatcccaa cgaaaaaaca
     9601 gaaatcagtg atttttctgt aaaacagacc atcgaattcc gcgcaccgga cggatcggct
     9661 aacctttatc ttctattagc cggtcttgta actgcagcag gacatggctt tgagttagaa
     9721 aacgcggtgg aatttgcaga taagaaatat gtggatgtgg atattcacaa atcgggaaat
     9781 gaagctaaga ccaatggatt tgaacattta ccgacttctt gttttgagtc cgctgaattg
     9841 ttggataaaa acagggaaac ttacataaaa cacggtgtgt tcagtgaagc agccattaac
     9901 aatataataa atcagctaaa atcatttaat gataagaaaa taagagaaga aattgctgcg
     9961 caaccggaga tgctccagaa cctcgtggat aagtactttt attgctgaca atatattcaa
    10021 atcaactgat taaacacaca aaaagccggt caattttcat tgtccggctt tttgaaaatt
    10081 atttgtatat caattagcct ttaatggctg caattccggg caatacaagt ccttcgatag
    10141 cttcgagaag agcaccacca cctgttgata cgtatgacat tttatcggca ataccgaatt
    10201 tatttacaca agcaacagaa tcacctccac ctacgagtga aaatgcaccg tttttggttg
    10261 cttcaactat tgcttcgcca acagcgcgtg atccatgggt aaagttttca aattcaaata
    10321 caccggtagg tccgttccag agtattgttt ttgaactctt gataacatct gcaaatatct
    10381 tttcagtttc aggaccgata tccagacctt cccaaccatc agggatttca ccaacgggtg
    10441 taaaaccggt atttgcatcg ttgctgaaag catctgcaat ttttgcatcg acagctagaa
    10501 ccagatttac attattctgt ttagctttct caatcaattc aagcgccagt tcaagtttat
    10561 catcttcaca aagcgatatc ccgattttac ctcccaatgc tttggtaaag gtatatgtca
    10621 taccacctgc gataatcaga ttatcaactt tggtgagcag attttcaata atttcaattt
    10681 ttgtagatac tttcgaacca cccatgatag ctgtaaacgg acgtgcagga ttatccatca
    10741 acttatctac agctgttact tccttgttca tcaggaaacc aaacatttta ttgtcggcat
    10801 caaagtaatc ggctatcaga gcggttgaag cgtgtgcacg gtgagcagta ccgaatgcat
    10861 cgtttacata acagtcggca taagaagcca gttttttagt aaattctttt tgtgaagctt
    10921 ttacttcctt tttggctgct gctttttcgt cttcggttgc agtttcgggc aagcgtggtt
    10981 tgccttcttc ttcagcatag aaacgaagat tttcgagcaa cagcgcttca ccgtctttca
    11041 actcagcaac cattttttca gtttcttcgc ccatacagtc tgcagcaaat ttcacaggta
    11101 cttcgagaag ttcagaaacc cggttaacaa tgactttcaa cgaatattta ggatccggat
    11161 tttttttggg acgtcccatg tgtgaggcaa taatcagcct tccaccatca gcaataatct
    11221 ttttcagagt cggcattgca gcacggatac gtgtatcatc ggtgatgttg aagttttcat
    11281 caacaggaac attgaagtca acacgaacaa atgctttttt accggcaaaa ttaaatttgt
    11341 caatcgtttg cataatcatt tttattttaa agttatatat tctttctttt tttggtttat
    11401 taaaatcggg ctctgaatgc ctgaaataag taaaatcaaa attaatccca tcacaaacaa
    11461 tgcgcctatc ccgacaatcc ataatccatc atgatttagg gtttgctttg ttctgaatat
    11521 taaaataaga aaatttatac cagcaattgc gactaaactt ccacaactga tagctgaaac
    11581 tttcaacaaa gaaagtgagt tttttttctt agtacctgta ttccaccaat caactgaaat
    11641 attgaaggga aaacataaca gtaaaataga tagtagtaag taaatattga ctgattgaaa
    11701 cgtctcccat gcagtttaat tcaccttgaa cttatattct attttgctta gttcgtcatt
    11761 tgcctttaca attttatttt tcagatcttc cttataatca attagttttc tctgtaatgt
    11821 ttcattacct acagatagaa tctgcacagc cagtatgccg gcgttcatag cgccattgat
    11881 accaacggta gccaccggaa tgcccggagg catctgtacg atagccagta aagcgtccag
    11941 cccttcgagg gatgaattga ctggcacgcc aataaccggt actgtagtca tggaagctat
    12001 cactccgggg agatgagcag ccatacctgc ggcagctatt attacctgta tgcctctgtc
    12061 gcaggctcct tttgcaaaga tttccacttc tgcgggcgta cgatgcgccg agagtgcctg
    12121 catttcaaag ggaatttcaa attcatccag aacttttgcc gttttgtcca ttaccggaag
    12181 atcggaagta ctgcccataa tgatactcac ttttgcattc atatccactc tgagttttat
    12241 tattgtctga ctttgctatc aaacatagac aacaaaattc ttaacctatc agtgcattat
    12301 attcgtctgc tgaaagcagt ccgtcaactt ctgcaacatt ggagcaggaa attttcacaa
    12361 tccagccttc gccgtaagga tcgttgttta caagttcggg agcatcttcc agatttgcat
    12421 tgaattcgag aatttcgccc gaaatcggga ggaacaagtc agaaacagtt ttaacggctt
    12481 caacagaacc gaaaacttct ccctgatcaa ttgtttcgcc tacagtttcc acttcgacaa
    12541 atatgatttc gcctaattcg ctctgcgcaa agtcagtaat tccaacaaag gcaaattcgc
    12601 cttcaacgcg tacccattca tgttctttgg tgtacttcag gttagccggt acattcataa
    12661 gattattttt tttaagttta gtatttagat ttagtaaata atttcacctt caaaaaagag
    12721 ttgcaaagtt acgaaaaaaa atcatggatt tatctattct tcaataaata caaatctgtt
    12781 tgtatcggat aatgatcgga agctttcagg ttaccccgtt tataatttgc cgactgaaaa
    12841 gccttatcgt acatgatgta atctattctg aaccgataga acgaatgctc aaatgtccag
    12901 ccaagtccgt tgcctgtttc tttgaaagag tctttcaaat ttcccagcac tttagtatat
    12961 gcatatgacg acggtacatc gttataatcg ccacaggaga ttaccttata aggtgattcc
    13021 ctaatcagtt ttgcgacaac gtcggcctgt tgagctctta cgcggtatgc aacactcagt
    13081 ttttgcgaca ggtattttgt aaccgaaccc agttcatcag tatcaaaccc atctttcagt
    13141 ttcgatgtca tctgtatatc ctgtgctgta atccgatttg attccaggtg attattcaca
    13201 attcgcagag tatcctctcc cactataata tctgtataaa tggtcaggtt aaaaattgat
    13261 ttataacctg catttcgctt ttcaataatc gggtattttg ataaagtaac cagcccgata
    13321 cccatactcc acttattgag ctgaaaactc acatgtttgt agggatatat tttgaagatc
    13381 cgttcgaagt cgtcgtaatt gaactgatag ctattttcgc tgatagcaca ttcctgcaga
    13441 cacacaacat cggcatctgt atcaagaata tattttatta caggatttgg atcattcttt
    13501 ttatgcttct tcatcattcc tgtattcatc gtattgtatg acagcacttt aattttcgtt
    13561 gaaactgtat caatatcagg ttttgccagg ttaaccggaa aagcactttt aacagttcca
    13621 taaaatatta ttatggcaat aagtgaaatc agaagatacc atttacggca gaatatccaa
    13681 aacacgataa aaaaaacatt gataattatc aacggcagaa gaaaaagtga tgcataagca
    13741 ggcagaagga gtttttcggg acttatgagc gagccaattt tcaccaatcc caaagccagc
    13801 acagctgtga tattgacaag taccattatc cactttataa aagcggtcat gggtcttcct
    13861 ttaatgctat tttttttcaa aaagttttct tttttcgtcg gcactcaggc tttcgtaacc
    13921 cgaggttttt attttatcaa gtatccggtc tatttcggct tcgtttcgcg ctttttgctg
    13981 gttatattcc tgcggcgaca ttcgttgcga atggaatttc ggagcttttt gcttcggtct
    14041 tggacgaaac aaatttacaa aaaagtcaac aatacgggtg ataaagtaag taatatctgt
    14101 tcctttttta taagtaacgg caaacaaata acctgtaagg gcacctccaa gatgagccat
    14161 ttcgccgcct ccattatttc cggtgaggct gaacacactg attagcaccg taataattgc
    14221 tatgtaaata agtttcaccc tgccaatcag caacaggttc atttcggcat tcggagctcg
    14281 aacagccgta gccacgacga ttgccatgat agaacccgaa gctccgagca acacactgta
    14341 atatgctgca gccgaataat atgggaatac attataagca atcacataaa ctgatgcagc
    14401 aattaaccct ccgagaatat aaagtgacag caactgtttc tccgaaaagt aggtcagaaa
    14461 caacctgccg aaccagaaga gcatcagcat attgaataaa atatgataga aacccttgtg
    14521 aagaaacata taggtaagag gcgtccagaa atgactgagt aatttattca gattggaggg
    14581 aattgccagc caattgatat attccgatgc atcaatattg aacaaacgga aaattacaag
    14641 aaaaatattt ataagtaaaa acacggcaac attcagataa atcaatttta tcagcgtatt
    14701 cccgttttta taggtaagtc ttaagttatc gaatatattc attgtgtatc gtaatattta
    14761 aaaattgatt acagaatgta cgtcggtatt cttagttaac tataaagtct tcctttcttc
    14821 ttccagtaat atataagcag tgcgccaaat aacataccac ccaggtgagc aaaatgcgcc
    14881 acgctatcgc cagagaactg agccacaccc agaaacagtt ccgccacacc ataaccaaga
    14941 acaaaccagc gagctttaat gggtaccggt ataaacataa gaaaaagcct ttcttcgggg
    15001 aaaagccaac cgaaagctag cagcaatccg aatactgagc ccgaggcacc aatggtaact
    15061 ggcatattca acagcagatc tttctgtttg atcacatcca gaacagtcat tcctgagata
    15121 tccccactaa aataagctct caagctatct acagaaccag aagatattgc cgaattaaaa
    15181 gccgaaacaa cagtatgaaa ctcgaaatac cagaacagtt gctgtacgat ggctgctccc
    15241 agtccgctta ccaggtaaaa aatcaggaac tttttaggtc cccacaccct ttcgagtatc
    15301 ccgccaaaca tgtacacggc aaacatattg aaaaaaatat gtgaaaaaga accgtgcata
    15361 aacatatatg taatcatctg agccggattg aacttttggg acgcgaaata gtgcatgccc
    15421 atcacatcgt tcaaatcaat gctgataccc cggcgttcca gaattcccgg aagcaggatt
    15481 gtagccacaa acaatacaac atttatggct agtatatttt tgacaatcgg aggtatgttt
    15541 gagaaaaaag aggatcggta attattcata aacagtattt ttacattcag aaaaaatcat
    15601 ctaaacactt ggaaatgaga tataatttgt caactaaaac atttttttga atttacaaaa
    15661 ttactaaaaa aacttcaaac tgctgttgcc gaacataaat tgcagaatat ttagaaaaaa
    15721 acaacattta ttttgcgaaa tgattgcttt ttgattttca attctctaca tttgtggcat
    15781 ataaaaatct tctatttact tataatctaa aaacttaaag aattatgaac aaagctgaac
    15841 tgatcaatgc aattgcagaa gcatccggta tgactaaggt tgcctcaaaa aaagcgctcg
    15901 acgctaccat taaaagtatc accggctcac tggccaaagg tgagaaagta agtctggttg
    15961 gtttcggaac tttctctgta gctgaacgtg ccgcacgtac aggcatcaat ccttccacaa
    16021 aaaaatctct gaaaattccg gcaaagaaag ttgctaaatt taaagccggt gccgatttgg
    16081 ctgatgcagt taaatagtca acagctaaac tgaacgtatt aaaaaggaca cttgactgag
    16141 tgcccttttt ttatttttca ggtacaacag aaattccgta tatttgcact ttcaaaaaaa
    16201 gaagtgatta tcagcactct tttgggggaa ttttctattt ataacaagaa caataagaca
    16261 caaatatgag cttagaatca acattaacag gaggtgtaat acaggcaata agcgaacttt
    16321 acggccagac gataacaccg gatatcatac agtttcagaa aacgaagaaa gaattcgaag
    16381 gagatattac ccttgtggtt tttccattcg ttaaacttgc ccgcaaatcg cccgaacaaa
    16441 ccggtgaaga aatcggtgcc tggctgaagt cgcgattcga aattattgaa aaatacaaca
    16501 tcatcaaagg atttgtcaat ttcagcatcg atacatcgta ctggattggg caactggcag
    16561 aaatacatca actgcctgaa ttcggaacaa ccgaaccaca ggacgatgct gcactgatga
    16621 tgattgaata ctcttcgccc aacaccaata aacccctcca cctcggacat atccgcaaca
    16681 acctgctggg cttcagcatt gccgaaatac agaaagccaa cggatggaaa gtggtgaaaa
    16741 ccaatatcgt taatgatcgg ggtatacaca tctgcaagtc aatgcttgcc tggaaacttt
    16801 tcggtaacgg agaaacacca gaaagatccg ccatgaaagg cgaccatctg gtaggtaaat
    16861 actacgtgga attcgacaag gcatataaag ccgaaatcag ggaactgatg gaaaaaggcc
    16921 tgagtgagga agatgccaaa aaagaagctc cgctcatcaa acaggcacag gatatgttgc
    16981 tgaaatggga acagggtgat gaagaagtac gctcgctgtg gcagatgatg aacggctggg
    17041 tatatgcagg ctttgatgaa acttaccgaa aaatgggggt tgattttgat aaaatatact
    17101 acgaatccga aacctacacc gaaggtaaat cgaaggtgga agaaggtttg cagaaaggtg
    17161 tattctacaa gcgggaagac ggttcggtgt gggcaaatct gattgacgac ggactggatg
    17221 agaaactgct ccttcgcgcc gacgggacat cggtttacat gacccaggat ataggtacag
    17281 cgaaacttcg cttcgacgac tatcccatta ataaaatggt gtatgtggta ggtaatgaac
    17341 aaaattatca ttttcaggtg ctttccattc tactggataa actgggattt gaatggggaa
    17401 aaggacttgt acatttctcg tacggaatgg tagaattgcc cgaagggaaa atgaaaagtc
    17461 gtgaaggaac tgtggttgac gccgatgatc tgattgaaga aatggttacg actgcccgtg
    17521 agacttcgca ggaactcggg aagcttgacg gagtatcgga ggaagaagct gagaatatcg
    17581 cgcggatggt tggtttgggc gcattgaaat actttatact taaagttgat ccgaagaaaa
    17641 acatgacctt caatccgaag gaatccattg acttcaacgg taacaccgga ccttttatac
    17701 aatataccca cgcacgaata aaatcggtgc tccggaaagc ggaagatctg ggaataaatt
    17761 ataatgaagc attgaatgtg caaacagtat tatcagaaaa agagaaatac ctgatacaac
    17821 tgctgaccga attccccgca acagtgaaac aggcgggtga tgaattcagt ccggcactga
    17881 tagccaacta tatttacgag cttgtgaaag agttcaatca gttctatcac gattactcca
    17941 tactgaagga agaaaacaag gatctgcgca attttcgtct cgtactgtcc gcatctatcg
    18001 gtaacgtaat taaaaccggc atggggttat taggaataga agtaccggat agaatgtaat
    18061 caaaactgat aaattacaat ttttcagaca gacataattt caacctcagt ttatattagc
    18121 tggggttgtt ttttttagaa atctatcatc gtaccttttc ttaattcaga cttttttaca
    18181 ataaatcgga tttatcattt ttttttgaag ctctcacaat tcggtcattt ccggagatat
    18241 cttttcggac ttcgacatta ataaaaccat gcgcttcgag gagcagtttg cattcatgtg
    18301 ccaagttttg gtgggtttcg aaaaaaattt tgcctccggg ggaaagatga gtgattccaa
    18361 attcggcaat tttcctgtag aaaataagcg gatcattgtc gtgaacaaac agcgccagat
    18421 gcggttcaaa gtcggtgaca tttttatcca tttcactgtg ttcggatgcg ggaatgtaag
    18481 gcggattgct gacaatgaga tcccactttc ggtcggttgg cgcgggattt aatatatcat
    18541 cctgaatgaa ttcaatatcc aggtgattga ttttcgcgtt ttcgcgagca acctccagcg
    18601 cttcagatga aatatccatt gccgcaacat ttgcaaaggg aaatttcttt tttaaggaaa
    18661 cagctataca tccactcccg gtaccaatat ctaaaaaaaa gccccctaaa tcccccctgg
    18721 gggacttaaa gacacctatc tcttccattc cttccccctt gtgggggaag taccgaaggg
    18781 gatgggggct tacccactcc accagttctt cggtctccgg ccttggaatc aaaacggaag
    18841 gattaacttt gaatttcaac ccgtaaaact cggtttcacc taatatatat tgtatcggtt
    18901 catggttttt cagttgcaga agataacggt cggcaagtat atttttctca tcatttaaga
    18961 tcaaatcttt attggaaagg agttggacac gtgaaagacc tgtaatattt tcaagcaata
    19021 aattcgcgat catatgtaat tcagcttcgg aatacatatc cgcaagtgag gtatttagtt
    19081 ttttcaggaa gtgttgcatt atgcaaaatt agtatttttc atgacgacac atccgatttt
    19141 tcaataaata atccatttgc ggtctgaaat gaaagaaagt tctgtaatta ttcctctagg
    19201 atccggtaaa tattacagtt tgcatgacat caaataattt tttacttttg tgggtcttcg
    19261 taaaaaatat caataattcc aaactgccat atgaatttca gaaaaatctt tacggcttta
    19321 cttctaataa ttatatcaac cggtcttaca gcccagaaat atcagaaacg cgaaatgcgg
    19381 gctgtgtgga ttgccactgt cgccaatatt gactggccaa gcaagagcgg acttacacct
    19441 actcagcaac gcatagaaat gcgtaaaatg ctcgatgatc tgcaagaatg caacatcaac
    19501 accatcgtgc ttcagatacg tcccaccgcc gacagttttt atccttcgga acttgagccc
    19561 tggtcgaact acctcaccgg caaacaggga caacgtccca atccgtttta cgatccactt
    19621 cagtttatca ttgaagaagc tcataaacgc tgcatggaag ttcacggatg ggtaaatcct
    19681 taccgtgtaa ccaatgctgc cgggcagata aaccgtctcg acaaaagcca tatttactac
    19741 aagagcaaag atctgattgt aaaatatggt gataaatact atttcaatcc gggtctggac
    19801 gaaacacgcg aattcctgaa caaagtggtg gaagatatag tagaacatta tgatattgac
    19861 gggctgcact ttgacgatta cttttacccc tatcccgagg gtggcaggga ttttccggat
    19921 gaagaaacct tccgggctaa tccgcgtgga tttacgaata aagccgattg gcgcagaaat
    19981 aacgtgaaca tggttattga agaactgcag aagaccatca aagacatgaa accctgggtt
    20041 cagtttggtg taagtccgtt tggtgtatgg cgcaatgaca acaaagacgc gcaggggtca
    20101 gcaacacggg caggaataac caactatgac gacctctacg ccgacatact gaaatggctg
    20161 cgtgaaggaa ccatcgatta tgtagttccg caactatact gggaaatagg gaaaaaagtg
    20221 gccgattacg aagtactcgt gaaatggtgg tcggatcaca gttacggcaa aaatctgtac
    20281 atcggacttt acgcttcggg gcttattgaa aacaaaacag aagcctggaa aaaaggaaac
    20341 gagctggctc gtcagctaaa gatgaaccga aattttccaa aaactgaagg agcagtctat
    20401 ttcagtgcca aagcgctgct gaaaaacccg cggggactgg ttgacacgct gaaaaacaat
    20461 tactataaat ttccggcgct gcaacccatc tgccacaaca taaaaggaga agcttcggca
    20521 caaccgcaga atatccgcgt agtgaaagac ggcaaagaag cctatatcct ttgggacgaa
    20581 gtggtggaag acggcggatg tcaggtggct tactacgtgg tgtacggctt taaaggcaag
    20641 aaaataggcg acatgaatga tcccgaaaat atcgtagcca taaccaccga aaattgcatt
    20701 gacctgaatg cctacgaaaa gaagttcaaa ggacgctgct cgtttgtggt aacagcagtt
    20761 aaccgcttcc ggcatgaaag tatacctacg cagggagtga caaggaggat gtaggtttag
    20821 cccccctgcc ccccaagggg ggagttgtag agggttaatt gaaaagttaa gtaattaatt
    20881 ggtcagtggt ctgtatcctt aagtctttat aaaatagtaa ttctccatat tcagcacact
    20941 caaaaatccc ccctttaggg ggttaggggg ctgttacaaa attcttcctg aaagtgcatc
    21001 cgtttttcca ttcggagcat ccgtaggcgg ttttgccttt aatgattttg cctttaccac
    21061 agacgggaca aacggttccg atgaggtcgt cggggttttc gggaagcatg gattgtgcta
    21121 caagctgcgc tttggtgcgg ttatcgcttt tcacgttgaa aa
//
LOCUS       DAOPYB010000032        20450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2702_length_20450_cov_13.510223, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000032 DAOPYB010000000
VERSION     DAOPYB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20450
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2702_length_20450_cov_13.510223"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..522
                     /locus_tag="PKH58_04185"
     CDS             172..522
                     /locus_tag="PKH58_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88256.1"
                     /translation="MKKPIILLFVLLCGNMFASAQIKIMLESEVEKKKNESAAAKQQA
                     AKPSYQIYADDYIPVKKNTGEKVIPKKTDARPEEKELTYAEITRKRQQRRKEGKLTDL
                     EEIQAQLKELEHLK"
     gene            753..1181
                     /locus_tag="PKH58_04190"
     CDS             753..1181
                     /locus_tag="PKH58_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88257.1"
                     /translation="MKKLILILFLAIFAGSNMPAQIKVVLVQDFEQMKANGTKNKPAA
                     NKRANINKTSSFNTREMTDDESNGTVRDDDNEGTGTEAQNLTSGASVPVKEAAQKGNT
                     GVSKTKNANVAADTDKKANAASGSDYVKRLRERQKAARGK"
     gene            1381..>2256
                     /locus_tag="PKH58_04195"
     CDS             1381..>2256
                     /locus_tag="PKH58_04195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNX88258.1"
                     /translation="MQQYLDILKTYWGYDEFRPLQAEIIQSIASGRDTLGLMPTGGGK
                     SLTFQVPALAMEGVCIVVTPLIALMKDQVENLKHKGISAAAIYSGMSHDEIMLALDNA
                     VCEAYKFLYVSPERLATRIFIAKVRSMHVCMIAVDESHCISQWGYDFRPSYLKIADIR
                     EILPDVPVLALTATATPEVVDDIQNKLHFREKNVFRKSFHRSNLIYVVRTVENKEEYL
                     LKILSSVSGTSVVYVRNRKKTKEIAGFLNDNGITAEHFHAGLKNETKDVRQARWKSGE
                     TRVIVATNAFGMGIDK"
     assembly_gap    2257..2356
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2357..2696
                     /locus_tag="PKH58_04200"
     CDS             <2357..2696
                     /locus_tag="PKH58_04200"
                     /inference="COORDINATES: protein motif:HMM:NF027450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HNX88259.1"
                     /translation="RYVERVKSVLDYAKEENHCRSKVLLGYFGETGAGACGKCDICLK
                     NSENLLTDEEFAQIKSAILQVLQEKELTIGNLAKKLSYKEIKVMQVIRLLLDQQQIIQ
                     NEQMKLQLPL"
     gene            2786..3706
                     /locus_tag="PKH58_04205"
     CDS             2786..3706
                     /locus_tag="PKH58_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNX88260.1"
                     /translation="MYKQNTFKYFRWHWGDRYDGWRVRNVDAVFSVIPYFLRTRMDAQ
                     NYFEERIPIDHIEAFIRDNKEEIPGLSIMHIVIAAMVRLVSQRPNLNRFVVWNKIFAR
                     NYLNFSLVIKRSLSDDGEETLIKPYFQPTDTLQDVARKIMEEQEKNQKVGHENSSDFL
                     SKFFGYLPDWLMRLVVFTCMWLDKVGLLPRFIEQASPWHSSIFLTNIGSIGVESIYHH
                     LYEFGNCSIFLAMGKKSQRHTVNKAGDVKPYKSILLKFVLDERICDGYYYASSMRLLE
                     KILKDPAVLLSPPEKVFVDEGVKWKRVDGK"
     gene            3789..4949
                     /locus_tag="PKH58_04210"
     CDS             3789..4949
                     /locus_tag="PKH58_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNX88261.1"
                     /translation="MQQEINPEKLIQSQSLKVPLEGDLGGFTSYSPLRGAGGISVRLP
                     AEWEPQSFVQLTWPHSATDWAYMLDEVNACFVNIAREISSREDLLIVCADEQEVRTLL
                     SNVDTTRIRFTQLPTNDTWARDHGGITVFEDGKPAVLDFTFNGWGMKFPANFDNLITK
                     RLFEENIFAGNVEYRNRLNFVLEGGSIESDGRGTILTTAECLLSDNRNNMTKDEVETR
                     LKEYLGAERVLWLHHGYLAGDDTDSHVDTLARLCNNHTIAYVKCDDEQDEHYEELKKM
                     EDELKQFYLYKASPSPEVNDLGEGYSLIPLPMADAVYDENGERLPATYANFLIINGAV
                     LMPVYNSPKDEIARQQLQKVFPDREIVCIDCNALIKQHGSLHCVTMQYTTAP"
     gene            5056..5928
                     /locus_tag="PKH58_04215"
     CDS             5056..5928
                     /locus_tag="PKH58_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HNX88262.1"
                     /translation="MKIALIQQSNTTDIAENIVKLEKNIRKVAAQGAALIVLQELHNG
                     LYFCQTEDVSVFEQAESIPGPSTEQFGKLAAELGVVIVLSLFEKRAPGLFHNTAVVLE
                     KDGNIAGIYRKMHIPDDPAYYEKFYFTPGDLGFEPVQTSVGKLGVLVCWDQWYPEAAR
                     LMALAGAEILIYPTAIGWESTDTIDEKNRQREAWITIQRAHAVANGIPVIACNRVGYE
                     ADPSGVTNGIQFWGSSFIAGQQGEILVQASDSEEENILFDLDLQRTEKVRRWWPFFRD
                     RRIDAYSDITKRWK"
     gene            6050..8887
                     /locus_tag="PKH58_04220"
     CDS             6050..8887
                     /locus_tag="PKH58_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNX88263.1"
                     /translation="MIYNSRRYFLLTILLCSVILTQGAIPAGYYHFVRGKKKAELKTT
                     LHEISSPLAVLDYGSGPGFTWQGFYSTDRNADGTVRDMYSNTVRSFSGYNSVDGMAIE
                     HSFPKSWWGAYENTAYKDLFHLYPADAITNGIKNNLPLGEVTGTPVFDNGKSKIGRNG
                     FGAVYSDNCFEPADEYKGDFARSYLYISTIYEDLTPLWNSPMLTNSVYPAWKPWAVDL
                     LLKWSTNDPVSQKEINRNDSIYAIQGNRNPFIDFPELSSYIWGKDTLKEFTFPEETGA
                     FLVTPRRKFNMDFGVILLNVTKTQRFTVQGMNITSPVTFSLQRNSPSLSLSANTITAE
                     QAHAGYTLQINFSPVISGTVTDTLLIQGGGLTEITRIPVRGVATSEFMATEPTEITPA
                     GGILHWLEDPYTTNYTVSLYKGDTKAGDLIISGYVEGASNDKALELFNGTGADVDLSK
                     YTLRKQTNGQGGFVVTQKLTGILPNNQTYVIVYNPPSSSNPVSNDLKSKANMFADSIC
                     AFNGNDAVALFRSGLQIDVVGKINGGADYSWGTDKILKRKADITHPANNFDLGQWTEY
                     AFDRFDKVGIHSISFDAESNYIFKNKSVGIVNELLVDNLLPEENYTYDVTSYRSGVVA
                     KTVNTQRLRTTGLETPIEMAATDISSTSFTANWEEVPYASGYFLDVYQLSGQLTTETE
                     GFTNGIPKNLGWSGSASGTYTSTASSGLNPPSIAFKINGDSLKTKPYPDIISKLSFMY
                     KFPSSATGSYLRVEAQNTSGFNKIDSLSYEGSTSTKYYPSYTFSSDKGYTAIKFTYTK
                     VTGNLALDDVTITHGQVDTLYVLKNKFVTGNQYPVSGLHAGNLYYYRLRAGRENSYSA
                     YSGTINVNTVSTATDKIINSIYRIGTLPDAIRIFNLKGNETVRLFSITGSVIAQEHVL
                     STAITLPVCSRGIFILQISDGIKTESYKIVR"
     gene            8912..9205
                     /locus_tag="PKH58_04225"
     CDS             8912..9205
                     /locus_tag="PKH58_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88264.1"
                     /translation="MKDQKKFFHSGILIENNEFETFDRPLLYAKSTDGLIFRNNTVKY
                     NTEFEQFHLNKYMFFFEKVKNIQIKNNRFEKGLDPEKVIRTELPESNSVILQK"
     gene            9224..11638
                     /locus_tag="PKH58_04230"
     CDS             9224..11638
                     /locus_tag="PKH58_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNX88265.1"
                     /translation="MKTRIIVAGFFVLSVLSSVSATQPKQELKLRYDTPADCWEKTLP
                     LGNGRIGMMPDGGIETDHIVLNDITMWSGSEQDALNPDAIKYLPQIRQLLLEGKNIEA
                     QDMMYRHFKCKGVGSGFGRGKDVPYGSFQMLGDLYIHQTFPTTRAEQKYSRSLSLSDA
                     VASTSFSKGDLQITREYLASHADNVLAIRIAANKKKSVSFELELSRTERANISAKDNI
                     LIMEGQLNDGYNGTKGVKFLTKVKIVHKGGTISMVGNRLSLQDADEAMVLVSASTNML
                     DKNYLQTVDELLANTEKRSYSNIKKHHIKKYQEKFNRVELNLGAQNTKDATDRQLNDF
                     QNNDNPAFAALYFQFGRYLMISGTNEHSLPLNLQGLWSNTVQTPWNGDYHLNINLQMN
                     YWLTEVCNLSELHKPLIEFTKSLVPSGTATAKTYYGAEGWTAHMMSNPWKFTAPGEHA
                     SWGATVTGGAWLCEHLWEHYAFTQDKDYLASVYPTLKGAADFFLSTLIKEPTHGWLVT
                     APSSSPENGFRQPGTSQTAYVCMGPAMDTQIMTELFSNVLSASRILEINDKTVSKIKE
                     TLPKFPPVQISKNGYLMEWLEDYEEVEPQHRHVSHLYGLFPANQISPNTTPQLAAAAR
                     ETLERRGDGGTGWSRAWKVNFWARLHDGNRAYKLLKSLLQPSIDINAPAGNRHSGGTY
                     PNLFCAHPPFQIDGNFGGTSGVAEMLIQSHDGYIELLPALPDVWHTGGFKGLRVRGGA
                     TIDAKWTNKKIEIFTITANVENTFWIKVPSNSSLLTKNGKALPIANGLISLKMKKGDK
                     ALIKFR"
     gene            12067..12480
                     /gene="rpsL"
                     /locus_tag="PKH58_04235"
     CDS             12067..12480
                     /gene="rpsL"
                     /locus_tag="PKH58_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNX88266.1"
                     /translation="MPTIQQLVRKGRVELIDKSKSPALDSCPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVMVRGGRVKDLPGVRYHVVRGTL
                     DTAGVSGRTQRRSKYGAKRPKPGQAAAAAKGKGKK"
     gene            12669..13145
                     /gene="rpsG"
                     /locus_tag="PKH58_04240"
     CDS             12669..13145
                     /gene="rpsG"
                     /locus_tag="PKH58_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNX88267.1"
                     /translation="MRKSKPKKRIVLPDPVFNESGVTKFVNHLMFDGKKSIAFGIFYD
                     SLEVVKNKLPNEEKSPLDIWKKALENITPLVEVKSRRVGGATFQVPTEIRPDRKESIS
                     MKNLILYARKRGGRSMADKLAAEIIDAFNNQGGAYKRKEDMHRMAEANRAFAHFRF"
     gene            13197..15314
                     /gene="fusA"
                     /locus_tag="PKH58_04245"
     CDS             13197..15314
                     /gene="fusA"
                     /locus_tag="PKH58_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNX88268.1"
                     /translation="MAKADLSLNRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHD
                     GAATMDWMEQEQERGITITSAATTTSWNYNDDKYKINLIDTPGHVDFTVEVERSLRIL
                     DGAVATFCAVGGVEPQSETVWRQADKYNVPRIGFVNKMDRSGADFYEVVRQVRDVLGA
                     KPCPIQIPIGSEEKFKGVVDLVKMKAILWHDETMGAQYDVEEIPAELLEEAQEWRDKM
                     LEIVAEYDDVLMEKYFDDPSTITVEEIQVAIRKATLSMEINPMICGSAFKNKGVQTML
                     DAVCAYLPSPLDTPEIVGKDPRFEDKDVVRHPDANEPLCALAFKIATDPYVGRLCFFR
                     VYSGQLPAGSYVYNTRSEKKERISRIFQMHSNKQNPVEVISAGDIGAGVGFKDIRTGD
                     TLCDEAHPITLESMDFPTPVIGISVEPKTQKDLDKLGIGLAKLAEEDPTFTVHTDEQS
                     GQTVISGMGELHLEIIIDRLKREFKVECNQGRPQVAYKEAISKAVEIREVYKKQSGGR
                     GKFADIIVKVEPADKDFDGSLQFVDVVKGGNIPKEYIPSIQKGFQLAMKNGVLAGFPM
                     DTLKVTLIDGSFHPVDSDQLSFEICAQIAYKTACAKAKPILLEPIMKMEVVTPEESMG
                     DVIGDLNKRRGQVEGMESSRTGARIVKAKVPLSETFGYVTALRTITSGRATSSMEFSH
                     YAEVSNAIAKQVVTEAKGKVELL"
     gene            15440..15745
                     /gene="rpsJ"
                     /locus_tag="PKH58_04250"
     CDS             15440..15745
                     /gene="rpsJ"
                     /locus_tag="PKH58_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNX88269.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKAREQFELSSYKRLIDIYSSTSKTVDALMKLELPSGVEVEIKV"
     gene            15919..16539
                     /gene="rplC"
                     /locus_tag="PKH58_04255"
     CDS             15919..16539
                     /gene="rplC"
                     /locus_tag="PKH58_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNX88270.1"
                     /translation="MPGLLGKKIGMTSVFSADGKNVPCTVIEAGPCVVTQIKTVETDG
                     YKAVQVGFQEKTEKHTNKAEAGRFKAAGVAPQRHLVEFKEFEQEFKLGDVITVDSVFE
                     ADSFVDVIGTSKGKGHQGVVKRHGFGGVGQATHGQHNRLRAPGSLGASSWPSRVFPGM
                     RMGGRTGGERVTMQNLQVLKVIPEHNLILVKGSVPGAKGSIVIINK"
     gene            16542..17171
                     /gene="rplD"
                     /locus_tag="PKH58_04260"
     CDS             16542..17171
                     /gene="rplD"
                     /locus_tag="PKH58_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNX88271.1"
                     /translation="MELNILDIKGKETGKKVLLDDAIFGIEPNDHAIYLDVKQYLANQ
                     RQGTHKSKGRSELAGSTRKLGRQKGGGGARPGDIKSGVRVGGGRMFGPVPRDYSFKLN
                     KKVKQLARKSALSYKAIENAITVVDNLNFEVPKTKEFVAMTNNLQLADKKALFILANG
                     NKNVYLSARNLPGAKVITVSELNTYSILHVKNLVFTEESVAALEQIFKA"
     gene            17184..17474
                     /gene="rplW"
                     /locus_tag="PKH58_04265"
     CDS             17184..17474
                     /gene="rplW"
                     /locus_tag="PKH58_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNX88272.1"
                     /translation="MAILVKPIVTEKMTQLGEKLNRYGFRVQKDANKIEIKKAIEEAY
                     SVTVTDVNTFIVAPKNKSRYTRTGIVKGATSAYKKAIVTLKEGDKIDFYSNI"
     gene            17480..18304
                     /gene="rplB"
                     /locus_tag="PKH58_04270"
     CDS             17480..18304
                     /gene="rplB"
                     /locus_tag="PKH58_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNX88273.1"
                     /translation="MAVRKLKPSTPGQRHKIIGTFDTITASAPEKSLVFGGGKSGGRN
                     HDGKMTMRYIGGGHKRKYRVIDFKRNKDGVPATVKTIEYDPNRSARIALLFYADGEKR
                     YILAPNGLQVGQVILSGAEAAPEVGNALMLQNIPLGTVVHNIELRPGQGASMARSAGT
                     FAQLTSREDKYVIVKLPSGEIRKVLGTCKATIGSVGNSDHALERSGKAGRSRWLGRRP
                     RVRGVAMNPVDHPMGGGEGRASGGHPRSRKGLLAKGYKTRHPKKQSSQYIIERKKK"
     gene            18327..18593
                     /gene="rpsS"
                     /locus_tag="PKH58_04275"
     CDS             18327..18593
                     /gene="rpsS"
                     /locus_tag="PKH58_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNX88274.1"
                     /translation="MSRSLKKGPFINLKLEKKVLAMNESGKKTVVKTWSRATMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFSPTRIFRGHGGKKK"
     gene            18640..19050
                     /gene="rplV"
                     /locus_tag="PKH58_04280"
     CDS             18640..19050
                     /gene="rplV"
                     /locus_tag="PKH58_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNX88275.1"
                     /translation="MGARKKITADAKKEANKSLYFAKLNGVPTSPRKMRLVADMIRGM
                     EVNRALGVLKFSNKEASIRMEKLLKSAIANWENKTEQKAENANLYVSSVYVDSATQLK
                     RLRTAPQGRGYRIRKRSNHVTLFVDTKNTNDNQN"
     gene            19056..19802
                     /gene="rpsC"
                     /locus_tag="PKH58_04285"
     CDS             19056..19802
                     /gene="rpsC"
                     /locus_tag="PKH58_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNX88276.1"
                     /translation="MGQKTNPISNRLGIIRGWDSNWYGGNNYGDSILEDSKIRKYLNA
                     RLAKASVSRIVIERTLKLVTITVCTARPGIIIGKGGQEVDKLKEELKKITDKEVQINI
                     FEVKRPELDAVIVASNVARQVEGKIAYRRATKMAIASTMRLGAEGIKIQVSGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYAHAEALTKVGIIGIKVWICRGEVYGKKDLAPNFTS
                     TKDAGRGGDRYHGDRPNNDRGGKGFKKRKK"
     gene            19829..20260
                     /gene="rplP"
                     /locus_tag="PKH58_04290"
     CDS             19829..20260
                     /gene="rplP"
                     /locus_tag="PKH58_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNX88277.1"
                     /translation="MLQPKKTKYRRQQDGRFKGNAQRGNQLAFGSFGIKTMQAKWLTG
                     RQLEAARIAVTRYMQREGQIWIRVFPDKAITRKPADTRQGKGKGNPEGFVAPVTPGRM
                     LFEVEGVSYEVAKEALRLAAQKLPVLTKFVVRRDYDTTSKA"
     gene            20266..>20450
                     /gene="rpmC"
                     /locus_tag="PKH58_04295"
     CDS             20266..>20450
                     /gene="rpmC"
                     /locus_tag="PKH58_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNX88278.1"
                     /translation="MKTREIKELNDKELQERLDAEKDNLARLKMNHAISPLDNPLQIK
                     DVRRTIARFATELRQRE"
BASE COUNT         6635 a         3924 c         4412 g         5379 t
ORIGIN      
        1 tctttacgat tatttctaca aatatttgtc gtgcaatata atatgcgttt ctgctgtttt
       61 ttttacaaaa ccggtaataa taagcatttg gatatttcgt ttaaagtgtg taatttggca
      121 ccgtttttgt gcagactacc tgcaagttat tattttataa ttattccttt tatgaaaaag
      181 ccgattatac tgttgtttgt actactgtgc ggaaatatgt ttgcatctgc tcagattaag
      241 attatgctcg agtccgaagt ggaaaagaaa aaaaacgaat ctgctgccgc taaacaacag
      301 gcagcaaaac cttcctacca gatttatgcg gatgattata ttcctgtaaa gaaaaatacg
      361 ggcgagaaag ttattcctaa gaaaactgat gcgcgtcccg aagaaaagga actgacctat
      421 gctgaaatta ccaggaagcg tcagcagcgg agaaaagaag gcaaactcac agatctggaa
      481 gaaatacagg cgcaactcaa agaactcgag cacctgaaat gacttttttg aaaaatattt
      541 aattcatgat ttatattccg gtcattattt tatatctttg acccaaaatc aaactgattt
      601 gaaaatatct tttaaaacag gcatgccaaa atacttttga catacaactt gtcccgatac
      661 atatcgggaa gggaatgatt acatggcgtg ttctcccgat aaatcgggac aggctatctg
      721 ataataatta aagaaataaa aaacataaac agatgaaaaa actaattcta attctgtttt
      781 tagctatttt tgccggtagc aatatgccgg ctcagatcaa ggtggtactt gttcaggatt
      841 ttgaacagat gaaagccaac ggaacaaaaa acaaaccggc agctaacaag agagcgaata
      901 tcaataaaac ttcctccttc aatacccgtg aaatgacgga tgacgaatca aacggcacag
      961 tacgagatga cgataacgaa ggaacaggta cggaagctca aaatcttacc tcaggtgcat
     1021 cggttccggt gaaggaagct gctcaaaaag gtaataccgg tgtatcgaag acaaaaaacg
     1081 ctaacgttgc tgcagatacc gataaaaaag ctaacgccgc ttcaggttct gattatgtga
     1141 aaagactgcg cgaacggcaa aaagcagcac gcggtaaata gttactgctt atacaactga
     1201 tatttcatac acaggaaaac aacttgacag aaacggttta acgtgtaaat tatcctgaaa
     1261 aagaaaaaat ttcgcggatt tcattcaaca ataaatatac taacgggaat cattcggttt
     1321 aacagacgga gtgattttct ttttttaatg tatatttgtg ttttaaaaat atagtacgaa
     1381 ttgcaacaat acctcgacat actgaaaacc tactggggct acgatgagtt ccggccacta
     1441 caggctgaaa tcattcaaag cattgcttct ggacgtgaca cattgggtct gatgcctacc
     1501 ggtggcggta aatcgctcac atttcaggtg ccggcattag ccatggaggg tgtttgtatt
     1561 gtcgttactc cgctgattgc acttatgaaa gatcaggtcg agaacctgaa gcataaagga
     1621 attagtgcgg ctgctatcta ttcgggcatg tcgcacgacg aaatcatgct ggcactcgat
     1681 aacgcggtgt gtgaagccta caaatttctc tatgtgtcgc ccgaacggct tgctacccgg
     1741 atttttatag ctaaagtcag gtcgatgcat gtctgcatga ttgctgtcga tgagtcgcat
     1801 tgtatatcgc agtggggata cgatttccgt ccgtcatacc ttaaaattgc tgatatccgc
     1861 gaaattcttc ccgacgtacc ggtgcttgct cttacagcca ctgccacacc cgaagtggtg
     1921 gacgatattc agaataagct gcatttccgt gaaaaaaatg tattccggaa aagctttcac
     1981 cgctccaacc tcatttatgt agtacgtacg gtcgaaaaca aggaggaata tctgctgaag
     2041 atattatcat cggtatccgg aacatcggtg gtatatgtac gtaaccggaa aaaaactaag
     2101 gaaatagccg gttttctgaa tgataacggg attactgccg aacacttcca tgccggtttg
     2161 aaaaatgaga caaaggatgt ccgtcaggcg cgctggaaat cgggagaaac acgtgtaatt
     2221 gttgcgacaa acgccttcgg gatgggcatc gacaaannnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnntcgc tatgtggagc gtgtaaaaag cgtgctggat tatgctaaag
     2401 aagaaaatca ctgccgaagt aaggtgttgc tcggatattt tggcgaaacg ggtgcagggg
     2461 cttgtggtaa atgcgatata tgcctgaaaa attcggaaaa tttacttaca gacgaagaat
     2521 ttgctcaaat taaatcagct atactgcaag tgcttcagga aaaagaatta acaatcggaa
     2581 atctggcaaa aaaactatca tacaaagaaa tcaaagtaat gcaggtaatc agacttttac
     2641 tggatcagca acaaattatt caaaatgaac agatgaaatt acagttgccg ctataatcga
     2701 aacataaaat aataatctga tgacctgaat atttaaaaaa gataaattat ctttgaaacc
     2761 aaataccatt aaaaccatat aagagatgta taaacaaaac acatttaagt attttcgctg
     2821 gcattgggga gatcgttacg atggatggcg cgttcggaat gtcgatgctg ttttttcagt
     2881 gattccttat tttctgagaa cccgtatgga tgctcagaac tattttgaag aaagaattcc
     2941 gatagatcat attgaagctt ttatcaggga taacaaagag gaaatacccg gtctgtcaat
     3001 tatgcatatt gtcattgctg caatggtccg cctggtttca cagcgaccca atctcaaccg
     3061 gtttgtagtc tggaataaaa tatttgcacg taattatctc aacttctctt tggttatcaa
     3121 gcgctcgttg tcggacgacg gagaagaaac actgataaaa ccatatttcc agccaacgga
     3181 tacgcttcag gatgtggcca gaaagatcat ggaagaacag gagaaaaacc agaaagtggg
     3241 tcatgagaac tcctcggatt tcttgtccaa atttttcgga tatttacctg actggctgat
     3301 gcgtttagtc gtgtttactt gtatgtggct ggataaagtg ggtttgctgc cccggtttat
     3361 agaacaggca agtccctggc acagcagcat tttcttaacc aatatcggat ccatcggcgt
     3421 tgagtcaatt tatcatcacc tctatgaatt tggcaattgc agtatctttt tggcaatggg
     3481 gaaaaaatcg cagcgccata ctgtcaacaa agccggtgat gtgaaaccgt ataaaagcat
     3541 attgctgaaa tttgtattgg atgaacggat atgcgatggc tattactatg cttcttctat
     3601 gcgtttgctt gaaaaaatac tgaaagatcc ggcagtttta ctttctcctc ccgaaaaagt
     3661 attcgttgat gagggtgtga aatggaaacg ggtggatgga aaatagcaaa tggttattgg
     3721 ttatttattt ttggttagta tagcaaaaat acaaataatt gagtgttaga taaaatgtgt
     3781 ttttaagaat gcaacaagaa ataaacccgg aaaaattaat tcagtcacag tcgctaaaag
     3841 tcccccttga gggggattta gggggcttta cttcctattc tcccctcagg ggggcagggg
     3901 gcatttctgt tcgtcttcct gccgagtggg aaccgcagtc gtttgtacaa ctcacctggc
     3961 cgcactccgc aaccgactgg gcctatatgc tcgatgaggt aaatgcctgt tttgttaata
     4021 ttgcacgtga aataagcagc agagaagacc tgctgattgt ctgtgctgat gaacaggaag
     4081 tcagaacttt actgtctaat gttgatacca cacgaatccg ttttacccaa ctgccaacca
     4141 acgatacctg ggcacgggat catggaggaa ttactgtttt tgaagacggc aaaccggctg
     4201 tactcgattt taccttcaat ggctggggaa tgaagttccc ggcaaacttt gataatctga
     4261 ttactaaaag attatttgag gaaaatatat ttgccgggaa tgtggaatat cgtaacagac
     4321 tcaatttcgt tctggagggt ggcagtatcg aaagcgacgg acggggaaca atcctgacca
     4381 ctgccgaatg tctgttgtcc gacaaccgga ataatatgac caaagatgaa gtggaaacca
     4441 ggcttaaaga gtatctgggt gccgagaggg tactttggct tcatcacggt tatctggccg
     4501 gcgatgatac cgacagtcat gtggatacgc tcgcccgttt gtgcaacaac cacaccatcg
     4561 cttatgtaaa atgtgatgac gaacaggacg aacactacga agagctaaaa aaaatggaag
     4621 atgaactcaa acaattttat ttgtacaagg caagtccttc tcctgaggta aatgatttag
     4681 gggaaggcta ttctttgata cctcttccga tggctgatgc ggtatatgat gaaaatggtg
     4741 agcgattacc tgccacatat gccaattttc tgataatcaa tggggcagtg ctgatgccgg
     4801 tatacaattc tcctaaagat gaaattgccc gtcagcaact tcagaaggta ttccccgatc
     4861 gcgaaatagt ctgtatagac tgcaatgcgc tgatcaaaca acacggctcg ctgcattgtg
     4921 taacaatgca atatacaaca gccccctaaa tccccctgaa ggggactttt ggaggtttgc
     4981 tgtaaattat gagatactat attatttaaa aaatgttttt tattaattcc ccctttaggg
     5041 ggttagggga caaatatgaa aatagcactc attcagcaat ctaacacaac tgatatagct
     5101 gaaaatatag tcaaattaga aaaaaacatc cgtaaagttg cggcacaggg tgctgcgctt
     5161 attgtgctgc aggaactgca caatggcttg tatttctgcc agaccgaaga tgtgtccgta
     5221 ttcgaacagg ctgaatccat tcccggacca tcaactgaac aatttgggaa actggcagct
     5281 gaacttggtg tggttattgt tttatcattg tttgaaaaac gtgctccggg attgtttcac
     5341 aatacagctg tagtgctcga aaaagacgga aacattgccg gcatctaccg caaaatgcat
     5401 atccccgacg atcccgcgta ctacgaaaag ttttatttta ctccgggcga tctgggcttt
     5461 gaacctgtcc aaacttctgt cgggaaactg ggtgtacttg tgtgttggga tcagtggtac
     5521 cctgaagcag ctcgcctgat ggcacttgca ggtgcagaaa tattgattta tccaacggca
     5581 atcggctggg agagcactga caccattgac gaaaaaaatc gtcagcggga agcgtggatt
     5641 acaatacagc gcgcccatgc tgtagcaaac ggaattccgg tgatagcatg caatcgggta
     5701 gggtatgagg ctgacccttc cggcgttaca aatggtatac agttctgggg cagcagtttt
     5761 attgcaggcc aacagggcga aatactcgta caggcctcgg atagcgaaga agaaaacatt
     5821 ttgtttgatc tggatttgca gagaacagaa aaagtaagac gctggtggcc attttttcgt
     5881 gaccggcgca ttgatgcata cagcgacatt accaaaaggt ggaaataaat cagccttata
     5941 agatattatg caaactcttt ttgtcaacag atgataaaaa gagttcgtgt ttgtaataca
     6001 taatcaaaaa tgtcttacat ttggcacatt ataaattaaa tgatgtcaga tgatatacaa
     6061 ttccagaaga tattttttac ttacaatatt attgtgttca gtaattctca ctcagggtgc
     6121 aatacctgca ggttattatc attttgtacg cggtaaaaag aaagccgaat taaaaaccac
     6181 acttcacgag atatcttctc cgttagccgt actggattat ggaagcggac ctggttttac
     6241 ctggcagggt ttttactcta ccgaccgtaa tgccgatgga actgtgaggg acatgtactc
     6301 taatactgtc cggagtttca gtggttacaa ttcggttgat ggaatggcaa tcgaacactc
     6361 atttcccaaa agctggtggg gagcatatga aaatacggct tacaaggatc tttttcatct
     6421 ctatccggcc gatgcaatca ccaatggaat aaaaaacaat cttccccttg gtgaagttac
     6481 gggaactccg gttttcgata atggcaaatc aaaaatagga agaaatggct ttggtgcagt
     6541 ctattctgac aattgctttg aacccgctga tgaatataaa ggtgattttg cccgttccta
     6601 cctctatatt tccaccatat acgaagattt gacacctttg tggaactcac ccatgctcac
     6661 caattcagtt taccctgcat ggaaaccctg ggctgttgat ctgctcttaa aatggagcac
     6721 gaatgatccg gtcagtcaga aagaaattaa ccgtaatgac agcatctatg caatccaggg
     6781 caaccgaaat ccgtttatcg attttccgga attatcttcc tacatctggg gtaaggatac
     6841 tctcaaagag tttacttttc cggaagaaac aggtgcattt ctcgtaaccc cgcgccgtaa
     6901 attcaatatg gattttggtg ttatattgtt gaatgtaact aaaacacaaa gatttactgt
     6961 acagggaatg aatattactt caccggtcac tttttcatta cagagaaata gtccgtctct
     7021 gtcactctca gcaaatacaa tcacagctga acaggcgcac gcaggttata cgcttcagat
     7081 aaatttttct ccagtcatat ccggtactgt taccgataca ttgttaattc agggtggagg
     7141 cttgaccgaa attacccgta ttccggtgag aggagttgcc acctcggaat ttatggctac
     7201 agaacctacc gaaatcacac ccgcgggcgg aatacttcac tggctcgaag atccctatac
     7261 gacaaattat accgtatctc tatataaggg cgataccaaa gccggcgatt taattatatc
     7321 agggtatgtt gaaggcgcat ccaatgataa agcgcttgag ctattcaacg gaacaggtgc
     7381 cgatgtggat ttatcgaaat ataccctgcg aaaacaaaca aacggacagg gtgggtttgt
     7441 ggtaactcaa aaactgacag gtatattacc caataatcaa acttatgtga ttgtgtacaa
     7501 ccctccatcg tcatctaacc ctgtgagcaa cgacctgaaa agcaaagcga atatgtttgc
     7561 agattcaatc tgtgctttta acgggaatga tgctgttgct ttgttccgca gcggacttca
     7621 gatcgatgtt gtaggtaaaa taaacggagg agccgactat agctggggta cggataaaat
     7681 actgaaaaga aaagccgata ttactcatcc ggctaataat tttgatttag gtcagtggac
     7741 ggaatatgca tttgatcggt tcgataaggt tggtatccat tcaattagtt ttgacgcgga
     7801 aagtaattat attttcaaaa ataaatcggt tggaattgtt aatgagttat tagtggataa
     7861 tctcctgcct gaagaaaatt acacctatga cgtcacttcg tacagatcgg gtgttgtcgc
     7921 gaaaaccgta aatactcagc gtttgagaac taccggactt gaaactccta tcgaaatggc
     7981 agcaacagat atcagttcaa catcctttac tgccaactgg gaggaagtac cctacgcctc
     8041 aggatatttt ctggatgtgt atcagttgtc ggggcaactg acaactgaaa cagaaggatt
     8101 tacaaatggt attccaaaga atttaggttg gtcaggctca gctagcggaa cttatacatc
     8161 gacagccagc tcaggtctta atccgccatc cattgcattt aaaattaatg gagattctct
     8221 gaaaaccaaa ccatatccgg atatcatcag taagctgagt tttatgtata agtttccttc
     8281 atctgcgaca ggttcatatt taagagttga agcacagaat acatccggat ttaataaaat
     8341 tgacagtctt tcatatgaag gaagtacctc tacaaaatat tatccatctt ataccttttc
     8401 atccgacaaa ggttacactg caataaaatt cacttatacc aaagtcacag gtaaccttgc
     8461 actcgatgat gtgacaatta ctcacggaca ggtagataca ctgtacgtgt tgaaaaataa
     8521 atttgtgacc ggaaatcagt atccggtgtc cgggcttcat gctggtaatc tgtattatta
     8581 tcgattacgt gccggtagag aaaattcata ttccgcttac tcaggaacta taaatgtcaa
     8641 tacagtaagt acggctacag ataagatcat taactcaatc tacagaatcg gtacattacc
     8701 ggatgcaatc aggattttta acctgaaagg aaatgaaacg gtaaggctgt tttccatcac
     8761 cggttcggtt attgctcagg aacatgtact ttcgactgcc attacacttc cggtttgttc
     8821 acgcggaatt ttcattctgc aaatatctga cggaattaaa accgaatcat ataaaatagt
     8881 cagataaaca attttcaaaa cttactgaaa tttgaaagac caaaaaaagt tcttccattc
     8941 agggatattg atagaaaata atgagttcga aacgtttgac cgaccgttgc tgtatgccaa
     9001 atctaccgat ggactgattt ttagaaacaa taccgttaag tacaacacag aatttgaaca
     9061 gtttcatttg aataaatata tgtttttctt tgaaaaagtg aaaaatatcc agattaagaa
     9121 taatcgtttt gaaaaaggct tagaccctga aaaggtcatt cgaacagagc ttcccgaatc
     9181 aaacagtgtt attctgcaga aataaacttt ataaaaagta tagatgaaaa cacgtattat
     9241 agttgccggt ttttttgttc tgtccgtttt gagtagtgta agcgcaaccc agccaaagca
     9301 ggaattgaaa ctgaggtacg atactcctgc cgattgttgg gaaaaaacgc tgccgctggg
     9361 caacggacga atagggatga tgcccgatgg tggcattgaa actgatcata ttgtgctcaa
     9421 cgatattacg atgtggtcgg gtagtgagca agatgcactc aatcccgatg ccataaagta
     9481 tttgccgcaa attcgccaac tattgctgga aggcaaaaat attgaagcac aggatatgat
     9541 gtacaggcac tttaaatgca agggagtagg ttcgggattt ggcagaggca aggatgtacc
     9601 ttatggcagt tttcagatgc tgggcgattt gtatatacat caaactttcc cgaccacaag
     9661 agctgaacaa aaatactcac gcagtttgag tttgtcggat gctgtagctt caacttcatt
     9721 ttcgaaaggc gatttgcaaa ttaccagaga atatctggct tctcacgccg ataatgtgct
     9781 ggcaatcaga attgcggcta ataagaagaa atcagtttcg tttgagttgg aactaagccg
     9841 cactgagcgg gctaatatat cagcaaaaga taatatcctg ataatggaag gccaactcaa
     9901 cgacggttac aatggaacta aaggtgtgaa atttctgacg aaagtgaaaa ttgtgcataa
     9961 aggtggtaca atctcaatgg ttgggaatag attgtcgttg caggatgctg acgaagccat
    10021 ggttttggta tctgcttcca ccaatatgtt ggataagaat tatcttcaaa cagtggatga
    10081 acttcttgcc aatactgaaa aacgttctta ttcaaacatt aaaaaacatc acattaaaaa
    10141 ataccaggaa aaattcaatc gggtggaatt gaatttaggc gctcaaaata caaaggatgc
    10201 caccgacaga cagttgaatg attttcagaa taacgacaat cctgcatttg cagcccttta
    10261 ttttcagttc ggcagatacc tgatgataag cggaacgaat gaacactcgt tgccactcaa
    10321 tttacaaggt ctctggtcaa acacagtaca aacgccatgg aatggcgatt atcaccttaa
    10381 tatcaatctt cagatgaact attggcttac cgaagtttgc aatctgtcgg agttgcataa
    10441 accactcatt gagtttacca aatctttagt tccgtcaggg acagcaactg caaaaaccta
    10501 ctacggagcc gaaggatgga cagcccacat gatgagcaac ccgtggaaat ttaccgctcc
    10561 cggcgaacac gcttcgtggg gtgcaacagt cactggtggt gcgtggcttt gcgaacatct
    10621 ttgggaacat tatgctttta cacaggataa agattacctg gcatcggttt atcctacact
    10681 caaaggagca gcagacttct ttctgtcaac tcttatcaaa gaacccacac atggctggtt
    10741 ggttactgca ccgtcgtcat cgccggagaa tggtttccgc caaccgggaa caagccaaac
    10801 cgcttacgta tgcatgggtc ccgctatgga cacccaaatc atgaccgaac ttttcagtaa
    10861 tgtgctttct gcttcccgga ttttggaaat taacgacaaa acggtatcaa aaattaaaga
    10921 aacactgccc aaatttccac ctgttcagat cagtaaaaat ggctacctga tggaatggtt
    10981 ggaagattac gaagaagtgg agccacagca tcgtcacgta tcgcacctgt acggattgtt
    11041 tcctgccaat cagatttcgc ccaacactac acctcagctt gcagcagcag caagagagac
    11101 actcgaacgt cgcggagatg gtggaacggg ttggtcccgt gcctggaagg tgaatttctg
    11161 ggccaggctt cacgatggaa accgggctta caaactgttg aaaagcttat tacaaccatc
    11221 aatcgatatt aatgcacccg caggcaaccg tcacagcggt ggtacgtatc ctaatctgtt
    11281 ttgtgctcat ccacccttcc agattgatgg aaattttggc ggaacgtcag gagttgccga
    11341 aatgcttatc caaagtcatg atggttatat tgaattactt ccggcactac ccgatgtatg
    11401 gcacacaggt ggtttcaaag gcttacgggt acgtggcgga gcaacaattg atgcgaaatg
    11461 gactaataaa aagattgaaa tatttacaat aacagcaaat gttgagaaca cgttctggat
    11521 caaagttcct tcaaattcct ccttacttac caaaaacgga aaagctctgc caatagccaa
    11581 cggtttaatt tctttgaaaa tgaaaaaagg agacaaggct ttaattaaat ttaggtagaa
    11641 aataaaaaat acacttacga atactactaa ttgattatta aaaaccattt cgggatgcaa
    11701 gtttgtaaat cccaaatgtg attaataact caaataacag gagatgataa atggctattc
    11761 ttcgctgtta aatgtgctcc attttcaagg acagttaact gttatccgaa ttgtaaattg
    11821 tcaggatata attttaattt ttcaaacaga aagactttac agatgcaccg cgaccatgtt
    11881 gttttattca gaaatatcta tctgataaaa aatacaatcc cctgacagct gggttttatg
    11941 gaaaatatta aaaaataatt ttcacaccta ttgtcattaa gaaaaaactt cgtacctttg
    12001 caacccaatt tggagaaaaa tctaaatgga taattaataa gtatttattt atcaaacaga
    12061 agtaaaatgc ctacaattca gcaattagtt agaaaaggaa gagtagaact catagacaag
    12121 agtaaatcac cggctttgga ttcatgtccg cagcgtcgtg gtgtttgtgt tcgtgtttat
    12181 actaccaccc ctaaaaaacc taactcggct atgcgtaaag tggctcgtgt acgtttaacg
    12241 aaccaaaagg aagtgaatgc ctacattccg ggagaaggac acaacctgca ggaacactca
    12301 atcgtaatgg ttcgtggtgg tcgtgtaaaa gaccttccag gtgtacgtta tcacgttgtt
    12361 cgtggtacat tggatacagc cggagtaagc ggtcgtacac aacgtcgctc caaatacggt
    12421 gcaaaacgtc cgaaaccggg acaagcagct gcagcagcca aaggtaaagg gaaaaagtaa
    12481 tttacgctga gtaaatctac ccgtctgaag aaaattcaga atttctaaca aaaaattaac
    12541 aaaatcaagt tcaaacaact tgtttgcgtt ttaaggtagc ggatgaaagg ttgagtaaat
    12601 ggttcggagg aactgttgaa gtaagactaa atgcaaccaa aaaacaaaaa acggaaattt
    12661 ttacaaaaat gagaaagtca aaaccaaaaa aaaggatcgt tttacctgat ccggttttca
    12721 atgaatcagg ggttaccaaa ttcgtaaacc acctgatgtt tgatggtaaa aaatcaattg
    12781 ctttcggtat attctacgat tcgttggaag tagtaaaaaa caaacttcct aacgaagaaa
    12841 aatcccccct cgatatctgg aagaaagcgc ttgagaatat cactccgctt gtagaagtaa
    12901 aatcacgccg tgtgggtggt gctactttcc aggttcctac agagattcgt cccgacagga
    12961 aagaatctat ttcaatgaaa aatcttatat tatacgcccg taaacgtggc ggtcgttcga
    13021 tggctgataa actggccgcc gaaattatcg acgcattcaa caaccagggt ggtgcataca
    13081 aacgcaagga agatatgcat cgtatggctg aagctaaccg tgctttcgct catttcagat
    13141 tttaaataaa cagcacaggt tagaggtatt aattaaaaca ggtataaaaa ttacaaatgg
    13201 caaaagcaga tttaagtcta aatagaaata tcggtatcat ggctcacatc gatgccggta
    13261 aaacaacaac atctgaacgt atcctttttt atactggatt aactcacaaa atcggtgaag
    13321 ttcatgatgg agctgccact atggactgga tggagcagga acaggaacgt ggtattacta
    13381 tcacatcagc tgctacaacg acatcctgga attacaatga tgataaatat aaaataaatc
    13441 tgattgacac tccggggcac gtggatttta ctgttgaagt agaacgctct cttcgcattc
    13501 tggatggtgc agtagcaaca ttctgtgctg taggaggcgt tgaaccacaa tcagaaactg
    13561 tttggcgtca agctgacaaa tacaatgtgc cgcgtatcgg ttttgtaaat aaaatggacc
    13621 gttctggtgc cgatttttac gaagtggttc gtcaggtaag agatgtgctg ggtgctaaac
    13681 catgcccaat tcaaattcca atcggatcag aggaaaaatt caaaggcgtt gttgacctgg
    13741 tgaaaatgaa agctattctt tggcacgacg aaacaatggg tgctcaatac gacgtggaag
    13801 aaattcctgc cgaattattg gaagaagctc aggaatggag agataagatg ctcgaaattg
    13861 tagctgaata cgatgatgtt ttaatggaaa aatactttga tgacccttct accattacag
    13921 ttgaagaaat tcaggtggct atccgtaaag ctacactttc gatggagata aatccaatga
    13981 tttgtggctc agctttcaaa aataaaggag tacaaactat gctcgatgct gtttgtgcat
    14041 acctgccaag tcctttggat actcccgaaa ttgtaggaaa agatccgcgt ttcgaagata
    14101 aagatgtagt acgtcatcct gatgccaatg aaccgttgtg tgcattagca tttaagattg
    14161 caacagatcc ttatgtagga cgtttatgct tcttcagagt gtattcaggt cagcttcctg
    14221 ccggctcgta tgtgtataat acacgctcag aaaagaaaga acgtatttca cgtattttcc
    14281 agatgcattc caacaaacaa aatccggttg aagttatttc agcaggtgat ataggtgcag
    14341 gagtaggatt caaagatatt cgcacaggtg acacactttg tgatgaagct catccgataa
    14401 ccctggaatc tatggacttc ccaactcctg ttattggtat ctctgttgaa ccaaaaacac
    14461 agaaagattt ggataaactg ggtattggac ttgcaaaatt agcagaagaa gacccaacat
    14521 ttacagtaca caccgacgaa caatccggac agacagttat tagcggtatg ggtgaacttc
    14581 accttgaaat tattatcgac cgtctgaaac gtgaattcaa agttgaatgt aatcagggac
    14641 gtcctcaggt ggcttataaa gaagctatca gcaaagctgt cgaaattcgt gaagtttaca
    14701 aaaaacaatc cggaggtcgt ggtaaatttg cagacattat tgttaaagta gaaccggctg
    14761 ataaggattt cgatggatca ttacaatttg ttgacgttgt taagggaggt aatattccta
    14821 aagaatatat tccatccatt cagaaaggtt tccagttagc tatgaaaaat ggcgttttgg
    14881 caggcttccc aatggataca ctgaaagtaa ctcttattga cggttcattc cacccggttg
    14941 actctgacca gttgtctttc gaaatttgcg ctcagattgc ttataaaact gcctgtgcaa
    15001 aagcaaaacc gatacttctg gaaccaatca tgaaaatgga agtggtaacc cccgaagaaa
    15061 gtatgggtga tgtaatcggt gacttgaata aacgccgcgg tcaggtagaa ggtatggaat
    15121 caagccgtac cggagccaga attgtaaagg caaaagtgcc gctttctgaa acattcggtt
    15181 atgtaacagc tcttcgtacc attacttcag gtcgtgcaac ttcttccatg gagttctcac
    15241 attatgctga agtttctaat gctattgcaa aacaagtggt aactgaagca aaaggtaaag
    15301 tggaactttt ataaataaat ttcaatagtt tatgatttcg gtcataaact attgcttgtg
    15361 taatagcaaa atacaaccgg ttagcgaatg actcttaacc gggaaactat aaacataaaa
    15421 ttaacaacta aaaacgacaa tgagtcaaaa aattagaatc aaactgaaat cttacgatca
    15481 caatctggtt gataaatcag cagaaaaaat cgtaaaaaca gtgaaagcta caggtgctgt
    15541 agtaagtggt ccaattccac ttccaactca caaacgtatt tttactgtaa accgttcgac
    15601 tttcgtaaac aagaaagcac gcgaacaatt cgaactttca tcttacaaac gtcttatcga
    15661 catttacagc tcaacaagca aaactgttga tgctctgatg aaacttgaat tgccaagcgg
    15721 tgttgaagtt gaaattaaag tctagaagat agttgcgagt aacaagctat gtattacctg
    15781 ttttttgtta tagaaaaatc atcggcaata agtagttttg tggctcgaaa attataaaat
    15841 tattaataaa aaattgtttc gagttgagtg atacttgtca ctcgtaactc gtaacttgta
    15901 actaaaaaaa catttgaaat gccaggatta ttaggaaaaa aaatcggaat gacatccgtt
    15961 ttcagtgccg atggtaaaaa tgtaccatgc actgttatcg aagcaggtcc ttgtgttgtt
    16021 actcaaatta aaactgttga aacagacgga tacaaagctg ttcaggtggg ttttcaggaa
    16081 aaaactgaaa aacataccaa caaagctgaa gcaggacgtt tcaaagcagc cggagtagct
    16141 ccgcagcgcc acttggttga gttcaaggaa ttcgaacaag aattcaaatt gggtgatgta
    16201 atcaccgttg atagtgtttt tgaagccgac agctttgtag atgtgatcgg aacttcgaaa
    16261 ggaaaaggac accagggtgt ggtaaaacgc cacggatttg gtggggtagg acaagcaact
    16321 catggtcagc acaaccgtct gcgtgctccg ggttctctcg gtgcatcttc atggccttca
    16381 cgtgtattcc ccggaatgcg tatgggtggc cgcacaggtg gtgaaagagt taccatgcaa
    16441 aacttgcagg tattaaaagt aattcccgaa cacaatctga tacttgtgaa aggatcggta
    16501 ccgggagcaa aaggttcaat cgtaatcatc aataaataac catggaactg aatattttag
    16561 atatcaaagg aaaagaaacc ggtaagaaag tattgttaga cgatgctatt ttcggtattg
    16621 agccaaatga ccatgctatt tatttagatg tgaaacaata tttggcaaat caacgtcagg
    16681 gaacacataa atcaaagggc agaagcgaac ttgccggcag tacacgtaaa cttggacgtc
    16741 agaaaggtgg cggtggtgct cgtccgggcg atatcaagtc aggggtacgc gtaggtggtg
    16801 gacgtatgtt tggtcctgtt ccgcgtgatt acagcttcaa actgaacaag aaagttaaac
    16861 aactcgcacg taaatctgct ttatcataca aagcaattga aaatgcaata acagtggttg
    16921 acaatttgaa ttttgaagta cccaaaacaa aggaattcgt agcaatgaca aataatttgc
    16981 aacttgctga taaaaaggca ctttttattt tagcaaatgg aaataaaaac gtatatttgt
    17041 cggctcgtaa tttaccagga gcaaaagtaa taactgtttc agaattaaat acttactcaa
    17101 ttcttcatgt taagaattta gtatttaccg aagaatcagt tgctgcatta gaacaaatat
    17161 ttaaagctta aggaggtaca gagatggcaa ttttagtaaa accaatcgtt acagaaaaga
    17221 tgactcagct gggcgaaaag ctgaaccgct atggctttag agtacaaaaa gatgcaaata
    17281 agattgaaat caaaaaagca atcgaagaag catatagtgt aacagtaacc gatgtgaaca
    17341 cattcattgt agcaccaaaa aacaaaagcc gttatacaag aaccggaatt gttaagggag
    17401 ctacatctgc ttacaaaaaa gcaatcgtta cactgaaaga aggagataaa atagattttt
    17461 atagcaatat ttaaacaaaa tggctgtacg taaattaaaa ccctcaacac cggggcagag
    17521 acacaagatt attggtacat ttgacacaat tactgcaagt gcaccagaaa aatcacttgt
    17581 atttggcggt ggtaaaagtg gtggtcgaaa ccatgacggt aaaatgacca tgcgctatat
    17641 cggcggtgga cacaagagaa aatacagagt aattgacttt aaacgtaaca aagatggtgt
    17701 gccagctaca gtaaaaacca ttgaatacga tccaaaccgt tcggctcgta ttgctttgtt
    17761 gttttatgct gatggcgaaa aacgttacat tcttgcacca aacggattgc aagtaggtca
    17821 agtaattctt tcgggagctg aagctgctcc tgaagttggt aatgcattaa tgctgcaaaa
    17881 cattccgctt ggaacagttg ttcacaacat tgaacttcgt ccgggacaag gagcttctat
    17941 ggctcgttct gccggaacat ttgcacagtt gacttcgaga gaagacaaat atgttattgt
    18001 caaattacct tccggcgaaa ttcgcaaagt actcggtact tgcaaagcta ccatcggaag
    18061 tgttggaaac tcagaccacg cattggaaag atcaggaaag gcaggtcgtt ctcgttggct
    18121 tggacgtcgt ccacgcgtga gaggtgtcgc tatgaacccg gttgatcacc ccatgggagg
    18181 tggcgaaggc cgtgcatcag gtggacatcc acgttcacgt aaaggcttgt tagcaaaagg
    18241 atacaaaacg cgtcatccta aaaagcaatc gagccagtat attattgaaa gaaagaaaaa
    18301 ataatcagaa aaattaagta aataatatga gccgttcgtt aaaaaaagga ccattcatca
    18361 acttgaagtt ggagaaaaaa gtgctggcga tgaatgaaag cggtaaaaaa accgtagtca
    18421 agacatggtc acgcgctaca atgatttcac ctgactttgt aggtcataca attgcagttc
    18481 acaatggaaa caaatttatc cccgtttata ttaccgaaaa tatggtaggt cacaagttgg
    18541 gcgaattttc accaacacgt atcttccgcg gtcacggtgg taagaagaaa taatccattg
    18601 aaataaacaa agaaaaaaat aaaaaatttc agaataacaa tgggtgcaag aaaaaaaata
    18661 acagccgatg ctaaaaaaga agcaaacaag agcctttatt ttgccaagct caacggtgtt
    18721 cctacatcac cccgcaaaat gcgccttgtg gcggacatga tacgcggaat ggaagtgaac
    18781 cgtgccttag gcgtgctaaa gttctctaat aaagaagctt ctatcagaat ggaaaaactg
    18841 ctgaaatctg caattgccaa ctgggaaaat aaaaccgagc aaaaagcaga aaatgcaaat
    18901 ttatatgtaa gttcagttta cgttgactca gccacacagc tgaaacgttt acgtaccgct
    18961 cctcagggtc gcggttatcg tatccgtaaa cgttcaaatc acgtaacact gttcgtggat
    19021 accaagaata ctaacgataa ccaaaactaa gcacgatggg acagaaaaca aatccgataa
    19081 gtaatcgttt aggaatcatc cgtggatggg attctaactg gtacggagga aacaattatg
    19141 gagactccat actcgaagac agcaaaatca gaaaatatct taatgcccgt cttgctaaag
    19201 ccagcgtatc acgcatcgtt atcgaacgta cacttaaact tgtaacaata accgtatgta
    19261 cagcccgccc cggtatcatt atcggtaaag gcggtcagga agttgacaag ctgaaagaag
    19321 aattgaaaaa aattaccgac aaggaagttc agatcaatat cttcgaagtg aaacgtccgg
    19381 aactggatgc cgtaatcgtg gccagtaacg tggcccgtca ggtagaggga aaaatcgctt
    19441 accgtcgtgc tacaaaaatg gcaatcgctt caaccatgag gttgggagca gaaggtatca
    19501 aaattcaggt atcaggtcgt ttgaacggtg ccgagatggc acgttcggaa atgtataaag
    19561 aaggaagaac tcctcttcat accttcagag cagatattga ttatgcacat gccgaagcat
    19621 tgactaaagt aggtataatt ggtattaaag tctggatatg ccgtggcgaa gtttatggta
    19681 aaaaagacct ggctcctaac tttacctcaa cgaaagatgc aggacgtggc ggtgaccgtt
    19741 accacggcga tcgtccgaat aatgaccgcg gaggaaaagg atttaaaaaa agaaagaaat
    19801 aactgtaatt tattgaattg aggatattat gttacaacct aaaaaaacaa aatacaggag
    19861 acaacaggac ggacgcttta agggcaatgc ccagcgcgga aatcagttgg ctttcgggtc
    19921 tttcggaatt aaaaccatgc aggcaaaatg gttaaccggt cgtcagctag aagctgcccg
    19981 tatcgctgtt acccgttata tgcagcgtga aggacaaatc tggatccggg ttttccccga
    20041 taaagccatc acacgaaaac cagccgacac acgtcagggt aaaggtaaag gaaatccgga
    20101 aggattcgtt gcacctgtaa ctccgggacg catgcttttc gaagtggaag gagtttccta
    20161 cgaagtagct aaagaagctt tacgtctggc agcacaaaaa ctgcccgtat taacaaaatt
    20221 cgtagtaaga cgtgactatg acacaacatc taaagcataa ggaacatgaa aacaagagaa
    20281 ataaaagaat taaacgacaa agaacttcaa gaacgtctgg atgccgaaaa agacaatctg
    20341 gcacgtctga aaatgaacca tgccatttct cctcttgata atcctttgca aatcaaagac
    20401 gtacgtcgta ctattgcccg ttttgcaact gaattacgtc agagagaaat
//
LOCUS       DAOPYB010000033        20132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2776_length_20132_cov_8.663595, whole genome shotgun sequence.
ACCESSION   DAOPYB010000033 DAOPYB010000000
VERSION     DAOPYB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20132
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2776_length_20132_cov_8.663595"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..1424)
                     /gene="lpxB"
                     /locus_tag="PKH58_04300"
     CDS             complement(291..1424)
                     /gene="lpxB"
                     /locus_tag="PKH58_04300"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNX88279.1"
                     /translation="MKYFLIAGEASGDLHASNLMRELKEIDAEAEFCFLGGDLMAAQG
                     GMLVNHYRDMAFMGFLAVVQNAGKIARNMDDCKTALAEFHPDVLILIDYPSFNLRIAR
                     YAKEKLNIPVYYYISPKIWAWKEFRIKLIKKYVDRMFTILPFETEFYNRHNYAVTYVG
                     NPSVDSIAARPNQEQTLNEFCSKNRLPEKPIIALLAGSRRQEIKSCLPVMLEAASRFP
                     GYQTVISGAPGIEPEFYTQLISQTGVNLVFNQTYELLQHAQVAVVNSGTATLETALIG
                     TPQVVVYKVAFGRLAMMIKPLVIKTKFVSLVNLIAGKEVVRELLAHHFTPETVYNELH
                     RLLNEPAYRSQQQKGYQEISRKLGAPGAARKAAEEMYRSLKHL"
     gene            complement(1561..1845)
                     /locus_tag="PKH58_04305"
     CDS             complement(1561..1845)
                     /locus_tag="PKH58_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88280.1"
                     /translation="MKKYNHFLYGFIPGLLLPVGFLWLYLKRFYPGNLPFFDILKELY
                     PGVMLGKLLLLSIMLNLVGVFIFYKQDCFKTGIGMMMGAMPYLIGALMMM"
     gene            complement(1876..2667)
                     /gene="surE"
                     /locus_tag="PKH58_04310"
     CDS             complement(1876..2667)
                     /gene="surE"
                     /locus_tag="PKH58_04310"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNX88281.1"
                     /translation="MKPTILITNDDGVSAPGIRVLTRVMQQIGNVIVVAPDGPRSGQS
                     NAITVHTPLRYRLLEKSEGLTIYSCNGTPTDCVKLAFNDIIETNPDLVVAGINHGANS
                     AINVIYSGTMGAVLEGCQHGVASIGFSLCDHSHEADFTAFEPYILRIANETLKNGLPE
                     NICLNVNAPIGEIQGIRISRQCRGVWVKEFARRTDPVGHDYYWLTGYFENHEPDAEDT
                     DEWALANSYVSIVPTTTDMTAHAMIEPIGKWELQTQELKTTDINR"
     gene            complement(2816..3544)
                     /gene="gldF"
                     /locus_tag="PKH58_04315"
     CDS             complement(2816..3544)
                     /gene="gldF"
                     /locus_tag="PKH58_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HNX88282.1"
                     /translation="MLSVLKKELRAFFSSATGYIVIGIFLLLTGLFLWVIPGEFNILD
                     SGYANTDGLFYLAPWLFLLLCPAIAMRFFAEEKQTGTWELIITKPLTTRQIVMGKYFA
                     GTILVILALIPTLIYYYTVSYLAEPVGNVDAGQFWGSFIGLLFLGSVYMAIGTFSSSL
                     TRNQIIAFIIGLVLCFFFFYGFDVLTSFVNSAVKMQFVEDLGIHAHYKSISRGVIDTT
                     DVIYFVVLSTLFLEFTVLKLKRSL"
     gene            3622..4851
                     /locus_tag="PKH58_04320"
     CDS             3622..4851
                     /locus_tag="PKH58_04320"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNX88283.1"
                     /translation="MILKKNVICLFLLSFMASSAIAQNTENLTFKGEFIYGTILKHTE
                     HLKNIVKGPVMGGEISVEWQSADGWHRYFNYPYLGVGAAYLNFGNPDTLGNALAIYPY
                     INFSIIRSGRFSVNFKAGAGLSYVTRTFADFRENVMRVQGDSLWLDYKKSNAAIGSPL
                     NVYFAAGFNADVPLSDNISLTAEYGWNHISNGMIKAPNSGINMLNAFIGLKYTPNYDA
                     RKNEYLRYYYAPDIPRNISVEITASGGLRQRYMRDGGEGYGKTFPIASLAAAVYKPIT
                     NFYRMGIGGDFFYDGVYTSGLSSGYKREYITVDELKNKLRFGLSWQNELIMGRLTAGI
                     HAGVYLFNPVKNLEPYGSVSSKGIIYPYNPEEEVGWFYTRVSGKYLLAKHIYASIGLK
                     THLQKAEFIEWGLGYRF"
     gene            complement(4884..7091)
                     /locus_tag="PKH58_04325"
     CDS             complement(4884..7091)
                     /locus_tag="PKH58_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX88284.1"
                     /translation="MTTTEIQSVYKQSIAFLSQGNLKNAFDKIIELSNELQSPVHREN
                     IENMQQSYRYMLQYFLKGMIDPERKLVYNKLIAKMFVQIADLREELFTRDSSNFEFSQ
                     KRYFPFTPQPTLSDLENGLNRMEKMALLHEAETVDTKHERGIQLEFETSIHFLFNYFW
                     LKSRYDSPEILNLYRLAMDEANTDSVAKSMLVSALTLNLWRYFDETKLLMLLDACNTS
                     DMYARERALAGLCFVLAKYNRFLPYFPAIRNRLVILADDNVITEQLQNIIIQIIATTE
                     TEQVSKKLQEEIFPELMKLRPLMDEGLNPENLMNLDEWGEINPQWEEMIEKSGASDKI
                     QEFAELQLEGADVYMSTFAMLKSFPFFSEFSNWFMPFDTDNTAVKPLFATDDKSLMTV
                     FLSSEVMCNSDRYSFCLSVLQMPELQRNLMKQSFGEATEQMEEISKDEALHSPNVQAK
                     NISKHYIQDLFRFFRLNPHRSDFADMFTKSLIMHKTFLFDLLSIDDTLKTNIAEYYFT
                     KKLYAQSLELFDEIKKEGEISAALYQKMGYAFQQTSRLAEALDAYRKADLIQPDDFWT
                     NRKIALCYRLMGNAEKALEVYRHTNFIKPDQFSVRLQIVSCLKELGKTEEALRELMQL
                     NEEYPDNVKIFREIMHCSFIAKNMAQAKYFSTLLLETDNLTPSDYCTAGHIEWCMNKQ
                     QKAAEYYIQSLRLLSNNWTQFDTLFQQDKNTLISNGISESDLLLMLDGLKYEE"
     gene            complement(7159..7860)
                     /locus_tag="PKH58_04330"
     CDS             complement(7159..7860)
                     /locus_tag="PKH58_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX88285.1"
                     /translation="MAKRQISLLRPIEDVLTFWDVLTPDERNFIQNSYTIHHYKKNEV
                     IQCEGDKPTHLMILAVGKTKVYKNGFGNRAQIVRMLKPGEHFGYRAIIANELNSTTVT
                     AFEDCTVYMISGENFLSILRHNNAFCYRFLEELSFDLAESDARTVNLTQKHIRGRLAE
                     SLLFLKDNYGLEEDGATISIYLSREDLANHSNMTTSNAIRTLASFVNESLIAMDGRKI
                     KIIDEEKLRKISKMG"
     gene            8118..9317
                     /locus_tag="PKH58_04335"
     CDS             8118..9317
                     /locus_tag="PKH58_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNX88286.1"
                     /translation="MNRFKLHLITILLILSGVNLINAQNIILSDSAVVSLITCSPGPQ
                     VYAKFGHTAIRLIEPTLGMDIVFNYGMFNFNKPNFYMKFIKGETDYELGVYETKYFLP
                     EYQDRNSSVTEQVLNLTKEEKQKLVDALFVNYEPQNREYRYNFVFDNCSTRPRDKILS
                     VINDKVGYRSAGEQQTYRNRIGFYTGENTWTKFGIDMLLGKDADVKSTRWTSMFLPEV
                     LSREFGAVEIVAADGTKRPLIKSEALLVERKDENVSSGILQQPLIVTLAILLIGVIII
                     LAEYRQKKYCKIVDSVLLIVTGLAGIIVFYLMFFSVHPLVQSNYNILWCNPLNVIVGI
                     LLWNKKLHTFINYFQLANVFLFFGALLVFVLSIQAINVASIPLIVLMLTRSLNWIAKQ
                     SKKLKSL"
     gene            9511..10683
                     /locus_tag="PKH58_04340"
     CDS             9511..10683
                     /locus_tag="PKH58_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX88287.1"
                     /translation="MKEKETLWNKNFSVACIANFLIACSFNLLVPVIPLYLTEVLHIP
                     ETNVGIVLSSYVLAMLFMRPFSGYMVDIFPRKALYIIGLSLFVTVYLGYYLVTAVLAF
                     VIVRFIHGVFWGITSVSANTVVIDIIPASRRAEGIGFFGMFNNLALAIAPYIALNIYH
                     SAGFHWLVTSAIGMGVIAIIFVSFISVPVRPKSVRPPLSFDRFILLPALPVLINQIIM
                     TSAWGTLIPFAALYGKSIGIQNSGILFLFLAFGIIVSRIFSGKMVDKGYIHPVIISAL
                     LIAAIGFVVYSQVHMLVAFCVSVFVVGVGFGMLLPALQTIYVNMATHDRRGTASSTYL
                     TGFDVGNGIGMLTGGYIISLWSFQTLYIIAAATCLLGIIVYLTSTIWVYTRKKLVE"
     gene            complement(10755..12428)
                     /gene="asnB"
                     /locus_tag="PKH58_04345"
     CDS             complement(10755..12428)
                     /gene="asnB"
                     /locus_tag="PKH58_04345"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HNX88288.1"
                     /translation="MCGIVCTFNIKQPVEVLRPQILQMSKIIRHRGPDWSGIFANEHA
                     ILAHERLSIVDPHSGKQPLYSKDGKLVLAVNGEIYNHREIRRQFEGKYEFLTQSDCEV
                     ILALYREKGPDFLEDLNGIFAFALYDIENNRFLIGRDHIGIIPLYQGWDKQGAYYVAS
                     ELKALEGYCNKIEEFLPGQYYYSPDNKPTKWYVRDWMEYDNVKDNSSSVEELRRALED
                     AVERQLMSDVPYGVLLSGGLDSSIISAVAKKFAAKRIESGNIEDAWWPQLHSFAIGLE
                     GSPDLAAARKVAEHIGSVHHEINFTVQEGLDAIRDVIYHIETYDVTTVRASTPMYLLS
                     RYIKSMGVKMVLSGEGADEIFGGYLYFHKAPNAEEFHKETVRKLSKLHLYDCLRANKS
                     LASWGVEGRVPFLDKEFMDVAMRLNPIDKMAGNGKIEKHILRLAFEDYLPKDIAWRQK
                     EQFSDGVGYNWIDTLREVATQKVTDDEMANVAYRFPIQPPMTKEEYYYRSIYSELFPS
                     DSAALCVPSVPSVACSTPVALEWDEAFKKNADPSGRAVSSVHEKGYKKK"
     gene            complement(12514..13935)
                     /locus_tag="PKH58_04350"
     CDS             complement(12514..13935)
                     /locus_tag="PKH58_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNX88289.1"
                     /translation="MGNPKAFMSIPRKEAGYRPVLERIYDFGEVEQTLNQEDRKLQAS
                     RCMDCGIPFCHWACPLGNRMPEWQDLIYKGRWKEAIEILHETNNFPDFTGRICPAPCE
                     KSCVLNLSEAPVTIRENEAAVTEVAFIEGMIKARPPKIRTGKKVAVVGSGPAGLAAAQ
                     QLNRKGHTVTVFEKDNSIGGLLRYGIPNFKLNKNVIDRRLTQLTEEGILFKTNTEIGN
                     TIAGADVMKEYDAVCLAVGAGHPRDISPAGRDLKGIYFAMDFLSQQNQVIMEEAMTIP
                     ERITAKDKHVLVIGGGDTGSDCVGTSIRQGAAKVTQIEILPKPPAGKNPDTPWPYYPN
                     ILKTSSSHQEGCERKWSLNTLEFKGKNGQVKEVVVEAVEWKKDENGRFTMISTGKTET
                     IKADLVFLALGFVHPVHEGLLTELGVAYDSRGNVAINKENQSSVSKVFAAGDAAMGAS
                     LVVRAIASGRKAAEDIHQYLIHN"
     gene            complement(14072..18598)
                     /gene="gltB"
                     /locus_tag="PKH58_04355"
     CDS             complement(14072..18598)
                     /gene="gltB"
                     /locus_tag="PKH58_04355"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNX88290.1"
                     /translation="MSLSPLQIDEYEQRSLYSFQNEHDACGVGLVINLSGDKSHEIVD
                     NGLQVLENMVHRGAESADNKTGDGAGILVHIPHEFILLQGIAVPSKGKYGTGLVFLPK
                     DVQKSNDCLNVIKDYVEKEGLTLLAVRDVPVNSEILGEISASNEPDIKQIFITGNLQQ
                     DELERKLYIVRKKIEKAIQQSKLAAERSFYIVSLSTKQMIYKGMLTSLQLREYFPDLS
                     NPNFTSRVALVHSRFSTNTFPTWDLAQPFRMIGHNGEINTIRGNRQWMESRESVLKSD
                     ILGNMKDLYPIVQPGMSDSASLDNVLEFLVMSGKSLPHAMAMLVPESFNDKNPISDEL
                     KAFYEYHSMLMEPWDGPATLLFSDGRFAGGMLDRNGLRPARYTITNDGCMIIASETGT
                     IDLEASNIKARGRLRPGKMLIVDTRTGQIFYDNELKDGLAKAFPYRDWLNKNCSNLDV
                     ISSGRSVNNDIPNYETLLTAFGYSVEDIHNLIIPMANEGKEPVSSMGNDVTLAAFSTK
                     PQRLFNYFRQQFAQVTNPPIDPIREELVMSLTGYVGSVHQNLLEQIPELSKIVKLKSP
                     ILTNTQFDILLNLRYKGFSTAVLPMLFDPKQGTKGLEKAIDELCISVEKAVDEGKNYI
                     VLSDRGVDADHAPIPSLLAVSAVHLYLVEKRKRMQIDIVVESAEPREVMHFALLFGFG
                     ANAVNPYLAFAVLNRKIKSGDIQLDFDTAKKNYIKSINKGLLKVLSKMGNSTLRSYRG
                     AHIFEAIGISSRVLNSYFKDISSKIEGIDLDDIAREVLQPFLEAFNTENNETFHLKNL
                     GNYAYRNEGEYHAWNPETISRLQIATKTGEYARYKEYASLVDNKPNPAFIRDLLEYKQ
                     NPIDISEVEPAENIMKRFCTGAMSYGSISREAHEAMAMAMNKIGGRSNTGEGGEDPER
                     YKKRNDGLSTRSAIKQVASGRFGVTAEYLVNADELQIKIAQGAKPGEGGQLPGYKVDK
                     IIAKTRHSIPGISLISPPPHHDIYSIEDLAQLIFDLKNINPKAIISVKLVSESGVGTI
                     AAGVAKAKADLILISGAEGGTGASPSSSIKHAGLPVEIGLAETQQTLVMNNLRGKIRL
                     QSDGQLKTGKDIIISALLGAEEYGFATSALIVLGCVMMRKCHMNTCPVGVATQNEELR
                     KRFLGRYEYLVNFFTFLAEDVREHLANLGFKTLDEAIGRADLLKRKHFPEFPKTEKID
                     LSRITFVPKEAETNALRKNAEQDHKLENILDRELINKSLPALDLCMPVEFQSKIKNTD
                     RTTGAMLSGEIAKRYGQSGLPDNTISIAFEGSAGQSFGAFLAKGITFSLHGDANDYLG
                     KGISGGKIIVTPPKGSTFPPEENILCGNTLLYGATSGEVYINGVVGERFCVRNSGATA
                     VVEGAGDHCCEYMTGGRTVVLGPTGRNFAAGMSGGVAYVLDEKGDFDYFCNMEMVELS
                     LIEDSSDNKELHGLIEAHYKHTNSPLAAKILHNWNEYVDKFIKVVPIEYKKVLEDEKI
                     EAIKRKIASVERDY"
     gene            complement(18748..>20132)
                     /locus_tag="PKH58_04360"
     CDS             complement(18748..>20132)
                     /locus_tag="PKH58_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HNX88291.1"
                     /translation="EFEAYKFGIPLKTRHNEVAPNQFELAPIFGECNLAVDQNLLVMS
                     IMKKVARRHGFRLLLHEKPFAGINGSGKHNNWSLGTDTGVGLLTPGKTPEENLQFITF
                     LVNILMAVYKNNALLKASIMTAQNAHRLGANEAPPAIVSAFLGSQISSVLDKLENSTS
                     DEAIVMGEKKGLSLGIAHIPEVLLDNTDRNRTSPFAFTGNRFEFRAVGSSANCSSAMA
                     TLNAAVAAQLIEFKASVDEKIASGATVQSAIFEVIKEYIKISKPIRFDGNGYSDEWKA
                     EAAKRGLDCETSVPEIYKAFTSEQSVKMFTSTGVLTEKELHARNEVKWETYTKWIQIE
                     ARVLGDLTMNHIIPLATRYQSFLLDNVSKIKAVFSGEKAEKLSKMDSDLIVEIGERIA
                     TIKNAVDDMVDIRRSVNKIEDEYEKAVAYHDKVLPYFDIIRYNTDKLELIVDDEMWTL
                     PKYREMLFIR"
BASE COUNT         5353 a         4307 c         4309 g         6163 t
ORIGIN      
        1 acaatgtttt ctatttcgaa tcgttcggca ataagttctt ccagcagggc aaacctcttc
       61 aggtgggagc acggctgacc ctcgatatct aagcttaaag caaatacatg caaaaaccag
      121 ccggcaatac gttgaaagcg cgtattgccg gttgttgttt taaacccggc atgggtaatc
      181 tgaatgaaac agagtagggt aaggactctt actggttgta agttctgttg ataagtaaga
      241 acctaatttg tggtaaatga atggcttggt gtctcggaga taatgctgat ttataagtgc
      301 ttcaaactcc tgtacatctc ttcagcagcc ttacgggcag ctccgggagc gccgagtttc
      361 ctgctgattt cctgataacc tttctgttgt tgtgaacgat aagcgggctc gttcagcaag
      421 cggtggagtt cattgtaaac agtttcgggg gtaaaatgat gcgccagcag ttccctgaca
      481 acctccttcc cggcgataag attaaccagc gagacaaatt ttgttttgat aaccagtggt
      541 ttaatcatca tggcaaggcg cccgaaagcc actttgtaca ccactacctg cggagttcca
      601 atcagcgccg tttcgagggt ggcggtgccc gaattgacca cggcaacctg agcatgctgt
      661 agcaattcgt aggtctgatt aaatacaagg tttactcctg tctgactgat cagctgggta
      721 taaaattcag gttcaatacc gggagcgccg ctgataacgg tttgataacc ggggaaacga
      781 ctggcagctt cgagcataac gggcaggcag ctttttattt cctgtctccg gcttccggcc
      841 aatagtgcta tgattggttt ttcgggaagt ctgtttttac tgcagaattc atttaatgtc
      901 tgttcctgat tgggacgggc ggctatgctg tccacactcg gatttcccac atatgttacc
      961 gcgtaattat gccggttgta aaattcagtt tcgaagggca gaatggtaaa catccggtca
     1021 acgtattttt ttatcagttt tatcctgaat tccttccagg cccagatttt gggagagatg
     1081 tagtagtaaa ccggaatatt cagtttttct ttggcgtaac gggctatgcg gaggttgaac
     1141 gacgggtagt caatcagtat cagtacatcg ggatgaaatt cagcgagtgc tgttttgcag
     1201 tcatccatgt tgcgggcaat tttacctgcg ttttgaacga cagccagaaa gcccataaaa
     1261 gccatatcgc gataatggtt caccagcatc cctccctgtg ccgccatcaa atcgccgccc
     1321 agaaaacaga actctgcctc agcgtcaatc tctttgagtt cgcgcatcag gttggacgcg
     1381 tgtaaatcgc ccgaagcttc accggcaatg aggaaatatt tcatatagaa ccaagacttt
     1441 tagaaccagg attattagat ttttaagagt aaaacaaata aacaaataga cttgtaaact
     1501 tgaatacgac ttaataaatt ttcaaattac cacattttca aattaccaca tttccaactt
     1561 tcacatcatc atcaatgccc caatcaaata aggcattgca cccatcatca tgcctattcc
     1621 cgttttgaaa caatcctgtt tgtagaaaat aaatactccc accagattga gcatgatgga
     1681 aagcagcagg agtttcccca gcatcacacc cggataaagc tccttcagaa tatcaaaaaa
     1741 aggtaaattg ccgggataga accgtttgag atatagccat aaaaaaccga ccggaaggag
     1801 taaaccgggt ataaaaccgt agagaaaatg attgtacttc ttcatttgtt tcagtcaggc
     1861 agttgattaa cggtattatc ggttaatgtc ggttgttttt aattcctgag tttgcaattc
     1921 ccacttcccg atgggttcaa tcatcgcgtg agccgtcatg tcggtagttg tgggaacgat
     1981 ggaaacataa ctgttagcaa gcgcccattc atcggtatct tcagcgtcgg gttcgtggtt
     2041 ttcgaagtaa cccgtcagcc agtaataatc atgtcccacc ggatcagttc ttctggcaaa
     2101 ttccttgacc caaactcccc tgcactggcg tgaaatccgg atgccctgta tttcgcctat
     2161 aggagcattc acgtttaggc atatattttc gggtaatccg tttttcagtg tttcgttggc
     2221 aatgcgcagt atatagggtt cgaacgcggt gaaatcggct tcgtgtgagt gatcgcacag
     2281 cgaaaatccg atggacgcga caccgtgctg acatccttca agtacagcac ccatggtacc
     2341 tgaataaatg acgttgatgg ccgaatttgc tccatgattt ataccggcaa caaccaggtc
     2401 gggatttgtt tcgatgatat cgttgaatgc cagtttgaca cagtcggtag gtgtgccgtt
     2461 acacgaatag attgtcagtc cctcgctctt ttcgagcaag cggtaccgca atggggtatg
     2521 aaccgtaatc gcgttcgatt gtcccgaacg gggtccgtcg ggggcaacaa caattacgtt
     2581 gccaatttgt tgcatcactc tggtcaatac ccgaattccc ggagcactga cgccatcatc
     2641 gtttgtaatt aatatagtag gtttcatatt tttttagtga tcagtaatca gtaatcagta
     2701 atcagtaatc agtaatcaga aattagtaaa cagtaatcag taatcagtaa tcagtaatca
     2761 gtaatcagag aacagtgaac tgtcaaacat taaacgttaa acgtcaaacg ttaaactaca
     2821 atgatcgttt cagtttcagt acggtaaatt ccaaaaacag ggtgctgaga accacaaagt
     2881 agatcacgtc ggtggtatca atcacacccc ggctgatgga tttgtaatgt gcatgaatgc
     2941 cgagatcttc gacgaactgc atttttacag cactattaac aaagctggtc agtacatcga
     3001 atccatagaa aaaaaagaag caaagtacca gtccgatgat aaaagcgata atctgattgc
     3061 gtgtcagcga ggaggaaaaa gttcctatag ccatatagac gcttcccaga aagagtaatc
     3121 cgatgaatga cccccaaaac tgtcccgcat ctacattgcc caccggttcg gccaaataac
     3181 tcacagtgta gtaataaatc agcgtaggga taagagccaa tatgaccaga attgttcccg
     3241 caaaatattt tcccattacg atttgtcgtg ttgtaagcgg cttggtaata atcagctccc
     3301 aggtacccgt ctgtttttct tcggcaaaaa aacgcatggc tattgccgga caaagcagta
     3361 gaaacagcca gggagccagg taaaacaaac catctgtatt ggcgtatccg gaatcaagta
     3421 tattgaattc acccggaatg acccataaaa acagtccggt cagcaacaga aatattccaa
     3481 taacgatata tcctgtagcg ctgctgaaga aggcgcgaag ttcttttttt aggacagata
     3541 acatttaatg agacattttt cttatttttg cgagttcaaa gatagcgaat attattctaa
     3601 aagttatatt aaccaccccg gatgattctg aaaaaaaatg taatatgcct tttcctgtta
     3661 agttttatgg catcgtcagc gatagctcag aataccgaaa acttaacttt caaaggagaa
     3721 tttatttacg gcacgatact gaaacacact gaacacctta aaaatattgt gaaagggcct
     3781 gtgatgggtg gtgagatatc agtggaatgg cagtcggctg acggatggca ccggtatttc
     3841 aactatccat atctgggtgt gggtgctgca tatctaaatt tcggaaaccc cgatacactc
     3901 ggaaatgcat tggcaattta tccatatatc aatttctcca tcattcgatc cggacgtttt
     3961 tctgtcaatt tcaaagccgg agccgggtta agttatgtca ccaggacatt tgctgatttt
     4021 agagaaaatg taatgagagt gcagggtgac tctctttggc ttgattataa aaaaagtaat
     4081 gctgcaatcg gttcacctct gaatgtgtat tttgcagccg gtttcaatgc ggatgtaccc
     4141 ctgtctgata atataagtct gacggctgaa tatggctgga atcatatttc caacggaatg
     4201 atcaaagcgc ccaattcggg gatcaatatg ctcaatgcat ttatcggtct gaaatataca
     4261 ccaaactacg atgcccgtaa aaatgaatac ctcaggtact attacgctcc ggatattccg
     4321 cgcaatattt ctgtggagat cactgcttcg ggtggattgc ggcaacgata tatgagagat
     4381 ggcggtgaag gatatggtaa aacctttcca atcgcttcgc ttgctgcagc ggtatataaa
     4441 ccgattacaa atttttatag aatgggtatt ggcggggatt ttttctatga tggtgtttat
     4501 acttccggat taagttcagg atacaaacgg gaatatatca cggtagacga attgaaaaat
     4561 aaactccgat ttggcctctc atggcagaac gaattgatta tggggcgatt aaccgccgga
     4621 attcatgccg gtgtttacct gttcaatcct gttaaaaatt tagaacccta tggatctgtt
     4681 tccagtaaag gcataatata tccctataat ccggaagaag aggtgggttg gttctacacc
     4741 cgcgtttcgg gaaaatacct gttagctaaa catatatacg catccatcgg gctgaaaacc
     4801 catcttcaaa aggccgaatt tatcgagtgg gggctgggat accggtttta aaagaacaaa
     4861 agccgttttc agcggcttta gttttactct tcgtatttca atccgtcaag cataagcaac
     4921 aaatcacttt cggatattcc gttggaaatc aacgtgttct tatcctgctg aaacagtgtg
     4981 tcgaactgtg tccagttgtt gctgagcaag cgaagagact gaatataata ttcggctgcc
     5041 ttttgttgct tgttcataca ccattcaata tgtccggctg tgcagtaatc agagggtgtg
     5101 agattatcgg tttccagaag cagtgtagaa aaatattttg cctgcgccat gtttttcgcg
     5161 ataaacgaac aatgcatgat ttcacggaag attttcacat tatcgggata ttcttcgttc
     5221 agttgcatta gttcgcggag ggcttcctcc gttttaccta attccttgag acaactgaca
     5281 atctgcaacc tgactgaaaa ctggtcgggt ttaataaaat tagtatgccg gtaaacctca
     5341 agcgcttttt cggcattacc catcaggcgg taacacaggg cgatcttgcg gtttgtccag
     5401 aaatcatcgg gttgtatcag atccgctttc ctgtaggcat cgagcgcttc agcaaggcgc
     5461 gatgtctgct gaaaagcata acccattttc tgatacagtg ctgccgatat ttcaccttct
     5521 ttctttatct cgtcgaatag ttcgagcgac tgcgcgtaga gttttttcgt gaaataatat
     5581 tctgctatgt ttgttttgag cgtgtcgtca atcgataaca aatcaaacaa gaaggttttg
     5641 tgcatgatga gcgatttggt aaacatatcg gcaaaatcgc tccggtgcgg attgagccgg
     5701 aagaaccgga acagatcctg aatataatgt ttcgaaatgt tttttgcctg cacattggga
     5761 gaatgcaatg cttcgtcctt actgatttct tccatttgct cggtggcttc tccgaatgat
     5821 tgtttcatca gattacgctg caactccggc atttgcaata cgctcagaca gaatgagtaa
     5881 cggtccgaat tgcacatgac ttccgaactc agaaaaacgg tcatcaatga cttgtcgtcg
     5941 gttgcaaaca ggggttttac agccgtatta tccgtatcga aaggcatgaa ccagttcgaa
     6001 aattcggaaa agaaaggaaa cgatttcagc atagcgaaag tactcatata cacatcggct
     6061 ccttcaagct gcaattcggc aaattcctga attttatcgg aagcacccga tttttcaatc
     6121 atctcttccc attgtggatt gatttcgccc cattcatcca aattcatcag attttcggga
     6181 ttaagtccct cgtccatcag cggacgaagt ttcatcagct ccggaaaaat ttcctcctgc
     6241 agttttttgg agacctgttc tgtttcggta gtcgctataa tttgtatgat aatgttctgg
     6301 agctgttcgg tgataacatt atcatcggcc aaaattacca gccggttgcg gatagcggga
     6361 aagtagggca ggaagcggtt gtacttcgcc agtacaaaac acagaccagc aagagctctt
     6421 tcccgtgcat acatatcgga ggtattgcag gcatcgagca gcatcaatag cttggtttcg
     6481 tcaaagtaac gccaaagatt gagcgtcaaa gcagaaacca gcatggattt agccacagag
     6541 tcggtattag cttcgtccat tgccagccgg tagagattga gaatttcggg agaatcatat
     6601 cttgatttga gccagaaata attgaaaaga aaatgaatag aagtttcaaa ttcaagctgt
     6661 attcctcttt cgtgttttgt gtcaacagtt tcggcttcgt gaagcagcgc cattttttcc
     6721 atcctattca gaccattttc caggtcggag agagttggtt ggggggtgaa gggaaaatat
     6781 cttttttgag agaattcaaa attggacgaa tcgcgggtaa acagttcctc gcgcaaatcg
     6841 gcaatctgta caaacatttt cgcaatcagc ttattataca ccaatttccg ttcggggtca
     6901 atcattccct tcagaaaata ttgcagcata taccgatagc tttgctgcat attttcaata
     6961 ttttcacgat gaaccggcga ctggagttcg ttgctcagtt caatgatttt atcgaatgcg
     7021 ttcttcaggt ttccctgcga aaggaaggct atcgattgtt tgtaaacgct ttgaatctca
     7081 gtagttgtca tagtctttat ttcggattga gaagcagatc aagctactga ttataagtcg
     7141 aatatgaata tgattgaatt aacccatttt gcttattttc cgcaactttt cttcatctat
     7201 tatttttatt tttcgtccgt ccatggcaat gagcgattcg ttaacaaaac ttgccagtgt
     7261 acggatggca ttggaagtgg tcatgttcga atgattggcc agatcttcac gtgacaaata
     7321 aatgcttatt gtagcgccat cttcttccag tccataattg tcttttagaa ataacaaaga
     7381 ctctgccaaa cgtccgcgaa tgtgtttttg tgtcaaattg acagtccggg catcggattc
     7441 tgccaggtcg aatgacaatt cttccagaaa acgataacaa aaggcgttgt tgtggcggag
     7501 tatggacagg aaattttcac cgctgatcat ataaacggtg cagtcctcga aagcggtgac
     7561 agtagtactg tttaattcat tggcgataat agcccgataa ccgaaatgtt cgccgggttt
     7621 gagcattctt acaatctgtg cccggtttcc aaagccattc ttatacactt ttgttttccc
     7681 gaccgccaga atcattaaat gcgtaggttt atcaccttca cactgaatca cttcattttt
     7741 tttgtagtga tgaatagtgt acgaattttg aataaaattt ctttcatcgg gtgtaagtac
     7801 atcccagaaa gttaatacgt cttcaatggg tcgaagtaat gaaatttgcc tttttgccat
     7861 ggcatcaatc agatatgttt gaaaactatg ttcttaagca caaatttaga aaaatatttc
     7921 aaatttgttg ccatttttgg cggaaaaact aacgttttgt aaagcttata cgttatatta
     7981 acgtttataa agataaaatt taacaatatt aaggattgat taatgctccg acgagattta
     8041 attttttttc ttacagtcac tgttatcatt caaaaaatga actatttttg cattgtataa
     8101 aaaaataccg gataactatg aaccgattta aacttcattt gattaccata ctgttaattc
     8161 ttagcggtgt aaatctgatt aacgcgcaaa atatcattct cagcgattcg gctgttgtaa
     8221 gtcttattac ttgttcgccc ggccctcagg tttatgctaa attcggtcat actgccatcc
     8281 ggcttattga acccaccctt ggaatggata tcgtattcaa ttacggtatg tttaatttta
     8341 acaagcccaa tttctatatg aaatttatca agggtgaaac ggattatgaa ctgggtgtgt
     8401 atgaaacaaa gtattttctg cccgaatatc aggatagaaa ttcatcggta accgagcagg
     8461 tgctcaatct gacaaaagaa gaaaaacaaa aactggttga cgcgcttttt gtgaactatg
     8521 aacctcaaaa cagagaatac aggtataatt ttgttttcga caactgttcc acccgtcccc
     8581 gtgataaaat actttcggta atcaatgata aagtcggata tcgttcggca ggtgagcagc
     8641 aaacctaccg gaacaggata ggtttttaca cgggagaaaa cacctggaca aaatttggta
     8701 ttgacatgct gttgggaaag gatgctgatg ttaagtccac ccgctggaca tccatgtttc
     8761 ttcccgaagt gttgagccgt gagtttggtg ctgtagaaat agttgcagca gacggaacaa
     8821 aacgcccgct tattaaatca gaagcactcc tggtcgaacg taaggatgaa aatgtaagtt
     8881 ccggaatact gcaacaaccg ttgattgtaa ctcttgccat tctgttaatc ggtgtgatta
     8941 tcattctggc agagtaccgg caaaaaaaat actgcaagat tgtcgacagt gtgttgctga
     9001 tagttaccgg actggctggt attattgttt tttatctgat gtttttctcg gtacatcctc
     9061 ttgtgcaatc caattacaat atcctttggt gtaatccgct aaatgtgatt gttggcatat
     9121 tgctctggaa taaaaaactt cacacgttca ttaactattt tcagctggca aatgttttcc
     9181 ttttctttgg tgccctgctt gtatttgtac tatccattca ggcaatcaat gtggcatcca
     9241 ttccactaat tgtattaatg ctgaccaggt cgttaaactg gattgccaag caaagcaaaa
     9301 agttgaaatc attataagaa acgcatcggg ctctttatcc cgaattccag aaaagaagaa
     9361 tacctcttcg cgcttttaat ccccgcacta ctgaaaatat ctttcagtcg ggaatctttt
     9421 tcttattacc ctgttgtttt attattggaa taccggtaaa ataaaaccgg taaaaaccgt
     9481 acttttgtat catcaaattt gcttgttgaa atgaaagaga aagaaacttt atggaacaag
     9541 aacttcagtg tcgcatgtat agcaaacttc ctgatcgcat gttcttttaa tttgttagta
     9601 ccggttattc ccttgtatct gacagaagtg ctgcacattc ctgaaacaaa tgttgggatt
     9661 gtactctcgt cgtacgtgtt agctatgctg ttcatgcgtc cgttttcggg atatatggtg
     9721 gatattttcc cacgtaaggc tttgtatatc attggcctgt ccctttttgt taccgtatat
     9781 ttaggttatt acctggttac ggccgtactg gcttttgtta tcgttcgttt tattcacgga
     9841 gtattctggg gcattacgag cgtatcggcc aacacggttg tgatcgatat catacctgca
     9901 tcaagacgag ctgaaggaat aggctttttt ggcatgttca ataacctggc tttggccatt
     9961 gcaccttata ttgcattgaa tatttatcat tcagccggtt ttcattggct tgtaacaagt
    10021 gccattggaa tgggcgtgat agctataatt tttgtgagtt ttataagtgt tcctgtacgt
    10081 cccaaatcag tgcgtccgcc actttccttt gatcgtttca tcctcttgcc ggctttaccg
    10141 gtgctgatca atcagataat tatgacttca gcctggggaa cactcattcc ttttgccgcc
    10201 ctgtacggaa agagtattgg cattcaaaat tcaggcattt tattcttatt tctggcattt
    10261 ggtatcatcg tctcccgcat cttctcagga aaaatggttg acaaaggtta cattcatccg
    10321 gtgataattt ctgcattgct tatagctgct atcggatttg tggtttattc gcaggttcat
    10381 atgctggtag cattttgtgt ttcagtcttt gtggtggggg ttggttttgg aatgctcctt
    10441 ccggctttgc agaccatcta tgtaaatatg gcaacacacg accggcgcgg tacggcttct
    10501 tccacctatc taaccggatt tgatgtgggc aatggcatcg gaatgcttac cggcggatac
    10561 attataagtt tatggtcttt ccaaacactt tatatcattg ccgctgccac gtgtttactc
    10621 ggaataatcg tgtatctgac cagtacgatc tgggtttata cccggaaaaa actggtggaa
    10681 taataagaac agaactcctc aaccataaaa aaaaccgggt tgcaattttc agcaaacccg
    10741 gcttaactaa acaactattt tttcttgtat cccttttcat gtactgaact gaccgcgcgt
    10801 cccgaaggat cggcattttt cttgaatgct tcgtcccatt ccagagcaac aggtgtactg
    10861 cacgccactg atggcacaga aggtacacat agtgctgcac tgtccgatgg aaataattca
    10921 ctgtagatgc tgcggtagta atattcttct tttgtcatgg gtggctggat aggaaaacga
    10981 taggctacgt ttgccatctc gtcgtcagtt accttttgtg tggcgacttc tctcagggta
    11041 tcaatccagt tgtaaccaac tccgtctgag aactgttcct tctgacgcca ggcgatatct
    11101 ttcggaagat agtcttcgaa tgccagacga agtatatgct tttcaatttt tccattaccg
    11161 gccatcttat ctatggggtt tagcctcatt gccacatcca tgaattcctt gtcgagaaat
    11221 ggcacccgcc cttctactcc ccatgatgcc agtgatttgt tggcccgaag acaatcatac
    11281 agatgaagtt tactcagttt acgtactgtt tctttgtgaa attcttcggc attgggagct
    11341 ttgtggaagt ataggtatcc tccgaatatt tcatcggctc cttcgcccga aagtaccatt
    11401 tttacgccca tggatttaat gtagcgtgac aggagataca tcggcgtact ggctctgact
    11461 gtagtcacat cgtaggtttc gatatgatag ataacatcgc ggatggcgtc caacccttcc
    11521 tgaacagtaa agtttatttc gtgatgtaca gatccgatgt gttcagcaac ctttcgtgca
    11581 gcagccagat ccggagatcc ttccagccca atagcaaatg agtgaagttg aggccaccat
    11641 gcatcttcaa tgtttccact ttcaatacgt ttagctgcaa attttttggc cacagccgaa
    11701 atgatagagc tgtcgagtcc acccgaaagc agtacaccgt aaggaacatc cgacatcagc
    11761 tgacgttcta cggcatcttc cagagcacgt cgaagttctt caacgctgga agaattatct
    11821 ttcacgttgt catattccat ccagtcgcgg acataccatt ttgttggttt gttatccgga
    11881 ctgtaatagt attgcccggg aagaaattct tcaattttat tgcaatatcc ttccaatgct
    11941 tttaattcag aagccacata ataagccccc tgtttgtccc aaccctgata aagcgggatg
    12001 atgccaatgt gatcgcgtcc gataagaaag cggttgttct cgatgtcgta gagcgcgaaa
    12061 gcaaaaattc cgttcaggtc ttccaaaaag tcaggacctt tttcacggta gagcgccaga
    12121 ataacttcac aatcggattg tgtgaggaat tcgtattttc cttcgaactg acgacggatt
    12181 tcacggtgat tatatatttc gccattgaca gcaagaacca gtttcccgtc tttactgtaa
    12241 agtggttgct ttccggaatg cgggtcaacg attgacaggc gttcgtgagc cagtatggca
    12301 tgttcattag cgaaaatacc cgaccaatcc ggaccacggt gacggattat ttttgacatc
    12361 tggagtattt gcggacgcaa tacttctact ggttgtttga tattaaatgt acatacaatg
    12421 ccacacatag ttggttatta gttaaaaagt taaaaattgt tttactaaaa aaacgggttt
    12481 gttgaaatta tagtgataat ccagatgttc ttatcaatta tgtataagat attggtgaat
    12541 atcttcggct gcttttcgtc cggaggcaat tgcccttact accagactgg cacccatagc
    12601 agcatccccg gcagcaaaga ctttcgaaac agagctttgg ttttctttat tgattgctac
    12661 gttaccacgc gaatcgtatg ctacaccaag ttcggtaagc aatccttcat gtaccggatg
    12721 aacaaaaccc aatgccagga acaccagatc agcctttatg gtttcggttt ttccggtgga
    12781 aatcatggtg aaacggccat tttcatcttt cttccattca actgcttcaa ccaccacttc
    12841 tttcacctgc ccgtttttac ccttgaattc cagggtgttc agcgaccatt tgcgttcaca
    12901 accttcctga tgtgagctgg atgttttaag aatattgggg taataaggcc agggcgtatc
    12961 cggatttttc ccggcaggtg gttttggcag aatttcaatt tgagtaacct tagcagcgcc
    13021 ctgacgtatg gaagtaccca cacaatcgga acctgtgtca ccaccaccga ttacaagtac
    13081 atgcttgtcc ttggcagtta ttcgctcggg aattgtcatg gcttcttcca tgataacctg
    13141 attttgttgc gaaagaaaat ccatagcgaa ataaatgccc ttcaaatcgc gtccggctgg
    13201 tgatatgtcg cgtggatgac ccgcacctac tgccagacat acggcatcat attctttcat
    13261 tacatcagca cccgcaattg tattacctat ttctgtattt gttttgaaaa gaataccttc
    13321 ttctgtcagt tgagtcagtc tgcggtcgat gacgttttta ttcagcttaa agtttggaat
    13381 tccatacctc agcaaacctc cgatgctgtt gtctttttca aaaacagtaa cggtatgacc
    13441 ttttcggttc agttgctgtg ctgcagccag acctgccgga ccggaaccaa cgacagcaac
    13501 ttttttacct gttcggattt ttggtggcct tgccttgatc atgccctcta tgaatgccac
    13561 ctcggttaca gctgcttcat tttcgcgaat tgtaacagga gcctcactca gattgagcac
    13621 acaggatttc tcgcagggtg ccggacagat tcgtccggta aagtccggaa aattatttgt
    13681 ttcatgcagt atttcaatgg cttctttcca tcttcccttg tatatcagat cctgccattc
    13741 gggcattctg ttgcccagcg ggcaagccca gtgacaaaat ggtatgccgc agtccataca
    13801 gcgcgatgcc tgcaatttgc ggtcttcctg attaagtgtc tgctccactt ctccgaaatc
    13861 ataaattctt tcgagaacag gacggtatcc cgcttccttg cgcggaatac tcataaatgc
    13921 ttttggattt cccatataag ttgtccccta accccctaaa gggggaaatt ggttagtagt
    13981 ttttaataat ctatttgtgt tgatatgctt gttaatgtgt gcttgagtta gttgcacacc
    14041 ccaaactccc ccttcagggg ccgggggaca attaataatc tctttccaca cttgcgattt
    14101 tacgcttgat agcttctatt ttttcatctt cgagtacttt tttgtattca atgggtacca
    14161 ctttgatgaa tttatcgaca tactcattcc agttgtgaag tattttcgct gccagcgggc
    14221 tgtttgtatg tttataatgt gcttctatta aaccgtgtaa ttctttgttg tcgctgctgt
    14281 cttcgatgag tgaaagttcc accatttcca tgttgcagaa ataatcgaaa tcgcctttct
    14341 cgtcgagtac ataagctaca ccgccgctca taccggctgc aaagttgcgt cctgttggcc
    14401 ctaatacaac tgtacgacct ccggtcatgt attcgcagca atgatcacct gctccttcaa
    14461 caacagctgt tgcacctgaa ttgcgcacac agaagcgttc accgacaact ccgttgatat
    14521 aaacttcgcc ggatgttgcc ccgtatagca atgtattgcc acaaagtata ttttcttccg
    14581 gtgggaatgt acttccttta ggaggtgtta cgataatttt tccacctgaa atgccttttc
    14641 cgagataatc gttggcatcg ccatgtaagc tgaatgtaat gccttttgcc aggaatgcac
    14701 caaaactttg tccggcagat ccctcaaaag cgatggagat ggtattgtca ggcaatcccg
    14761 actgtccgta acgtttggca atttcacccg agagcatggc acctgtagta cgatccgtgt
    14821 tctttatttt agactgaaat tctacaggca tacacaggtc gagagctggt agagatttat
    14881 tgataagctc ccggtcaagt atgttttcaa gcttgtgatc ctgttcggcg tttttgcgga
    14941 gtgcatttgt ttcagcttct tttggaacaa atgttatacg gcttaaatca attttctcag
    15001 tttttgggaa ctccggaaaa tgttttcgtt tcaacagatc ggctctaccg atggcttcat
    15061 ccagtgtttt gaagccaaga tttgccaaat gttcacgaac atcttcagcc aggaatgtaa
    15121 agaaattgac caggtattca tatcttccca ggaagcgctt acgcagttcc tcattttgtg
    15181 tggctacgcc cactggacat gtgttcatgt gacatttacg catcatcaca catcccagca
    15241 caatcagtgc actggtggca aaaccgtatt cctcagctcc aagaagagct gaaataatga
    15301 tgtcctttcc ggttttgagt tgtccgtctg attgtaaacg gatttttccg cgcaggttat
    15361 tcattaccag tgtttgctgt gtttcagcaa gtccaatttc tacgggaagt cctgcatgtt
    15421 tgatggaact tgacggactt gcgccggtac cgccttcggc accgctgata aggattaaat
    15481 cggctttggc tttggctaca cctgcggcta ttgtacctac accgctttca gataccagtt
    15541 taacgctgat gatggctttc ggattgatgt ttttcaaatc gaaaatcagt tgagctaaat
    15601 cttcgatgga gtaaatatcg tgatgcggtg gtggtgaaat gagcgaaatt cccggaatgg
    15661 aatgtcttgt tttggcaatg attttatcca ctttataacc cggaagttgt ccgccttcgc
    15721 ccggttttgc accctgagct atttttatct gtagttcgtc tgcattcacc agatattcgg
    15781 cagtcactcc aaaacgtcct gatgctacct gtttgatggc acttcgtgta ctcagcccgt
    15841 cgttccgttt tttataacgt tcgggatctt ctccaccttc tccggtattg cttcttcctc
    15901 cgattttatt catagccata gccatagctt catgagcttc gcgactgata gaaccataag
    15961 acatggcacc tgtacagaag cgcttcatga tattttccgc cggttccact tccgaaatat
    16021 ctatcggatt ttgtttgtat tcgagcaaat cgcggataaa tgcaggattc ggtttattgt
    16081 ccaccaaaga cgcatattct ttatatctgg catattctcc tgttttagtg gcaatttgca
    16141 gtcgtgaaat tgtttcggga ttccatgcgt gatattcacc ttcattccgg tatgcataat
    16201 ttcctaagtt tttcagatgg aatgtctcgt tgttttcagt gttgaatgct tccaggaaag
    16261 gttgcagtac ttcacgcgca atgtcgtcaa ggtcaatacc ctcaattttt gaggaaatat
    16321 ccttgaaata tgagttcaat actcttgatg aaatgccgat agcttcaaaa atatgcgcac
    16381 cgcggtagct tctcaatgtg gaatttccca ttttggaaag taccttgagc aatcccttat
    16441 ttatagattt gatataattc tttttggctg tgtcaaaatc aagttgtata tcacccgatt
    16501 taattttacg gttgagtaca gcaaatgcga ggtaaggatt tacagcgttg gccccgaaac
    16561 cgaacaacag agcaaaatgc atcacttcac gcggttcggc gctttccact acaatatcaa
    16621 tctgcatcct cttacgtttc tccacaaggt ataaatgtac tgctgatacg gccagcaggg
    16681 agggaattgg tgcatgatca gcatcaacac cacggtcgct gagtacgatg taatttttac
    16741 cttcatctac cgccttttcc actgaaatac aaagttcatc aatagctttt tcaagccctt
    16801 ttgtaccttg tttcggatcg aaaagcatgg gtaaaacagc cgttgagaag cctttatatc
    16861 tcaaattcag taatatgtca aactgagtgt tggtcaaaat cgggcttttc agttttacaa
    16921 tcttggacaa ttcgggaatt tgttcaagca gattctgatg cacggagcca acataacctg
    16981 taagtgacat cacaagctct tcgcgtatcg ggtcgatagg tgggttagtt acctgagcaa
    17041 actgctgtct gaaatagttg aacaaacgtt ggggtttggt cgaaaatgca gcgagtgtaa
    17101 catcgttacc cattgatgac accggttctt tcccttcgtt tgccattgga atgatcagat
    17161 tatggatatc ttctactgaa taaccgaatg cagtcagaag agtttcataa tttggtatgt
    17221 cgttgttaac gcttctgccg gatgaaataa catcgaggtt gctgcaattc ttgttcagcc
    17281 agtcgcggta ggggaatgct tttgcgagtc cgtctttaag ttcgttatca taaaagatct
    17341 gaccggttct ggtatcaaca atcagcattt tacccggtct taaccttcca cgtgctttga
    17401 tattcgaagc ttcgagatct attgttcctg tttcggaagc aataatcata caaccgtcat
    17461 tcgtgatggt gtagcgtgct gggcgtagtc cgttgcggtc aagcatacca ccggcaaatc
    17521 gtccgtcgct gaaaagcagt gttgccggtc cgtcccaggg ttccatcagc atgctgtgat
    17581 attcataaaa tgccttcaac tcatccgaaa taggattttt gtcgttgaag ctttcaggca
    17641 caagcattgc cattgcatgt ggaagacttt ttcccgacat taccagaaat tccagtacgt
    17701 tgtcgagcga agcgctatcg ctcatgccgg gctgaacaat gggatatagg tctttcatat
    17761 ttcccaggat atccgatttc agaacacttt cgcggctttc catccactgc cggtttccac
    17821 gaatggtatt gatctcacca ttatgaccga tcatgcggaa tggttgagcc agatcccagg
    17881 tggggaatgt gttggtgctg aagcgtgagt gaacaagtgc tacgcggctg gtaaaattcg
    17941 gattgctgag atcaggaaaa tattcacgta actgcaggga agttagcatt cccttgtaaa
    18001 tcatttgttt ggtagaaagg cttacaatat agaaactacg ttcagcagca agttttgact
    18061 gttgtatggc tttctcaatt tttttacgga caatgtaaag tttacgctct aattcatcct
    18121 gttgcagatt gccggttata aaaatctgtt tgatgtcagg ctcgttagag gcagatattt
    18181 ctccgagaat ttcactgttt accggaacat cgcgtactgc tagtagtgtt aaaccttctt
    18241 tttcaacata atctttgatg acattcaggc aatcgttgga tttttgaacg tctttaggaa
    18301 ggaaaacaag tccggtgccg tatttgccct ttgatggtac ggcgattccc tgtaaaagaa
    18361 taaattcatg aggaatatgc accagaatac cggctccgtc accggttttg ttgtcggcgc
    18421 tttcagctcc gcggtgtacc atattttcaa gtacctgaag gccgttatcg acaatttcat
    18481 gcgatttatc tccgctgaga ttgataacaa gacctacgcc gcatgcatcg tgttcgtttt
    18541 gaaatgagta tagcgaacgc tgctcatatt catctatttg taatggactt aaactcataa
    18601 tttgtttgga agtactgaca ttgctttctg ataaaaaagc gtcaatggca atcgtttgtt
    18661 tgttaagttt tattgttgaa aatagtgaga aaactttata ttcagaattc tcactatttt
    18721 tttttaagta tgggagaatt ctatttttta tctgataaac agcatttctc tgtatttagg
    18781 taatgtccac atttcatcat ccacaatcaa ctcaagttta tcggtgttgt agcggataat
    18841 atcgaaatag ggaagtacct tatcatgata tgcaactgct ttttcgtatt catcttctat
    18901 tttgtttaca gagcgacgga tgtcaaccat atcatcaact gcattcttga ttgttgcaat
    18961 tcgttcacct atttctacaa tcaggtcaga atccatttta ctgagttttt cagctttttc
    19021 accgctgaat acagctttta ttttcgatac attatccaga agaaaactct gatagcgtgt
    19081 tgcaagcgga atgatgtggt tcattgttaa atcacccagc acacgtgctt caatttgtat
    19141 ccacttggtg taggtttccc atttcacttc attacgggca tgaagttctt tttcggtaag
    19201 tacaccggtt gaagtgaaca ttttcacgct ttgctcgctt gtaaatgctt tatagatttc
    19261 cggaacactg gtttcgcaat ccagaccgcg tttggctgct tctgctttcc attcgtcgct
    19321 ataaccatta ccatcgaaac ggattggttt ggatattttg atatattctt tgataacttc
    19381 gaaaatggca ctttgaacag ttgcgcctga agcaattttt tcatctactg aagctttgaa
    19441 ctcaatgagt tgtgcggcaa cggcagcatt aagagtggcc attgctgaag agcagttggc
    19501 tgaagaaccc acagcacgga attcgaaacg gttgccagtg aaggcaaacg gtgaggtacg
    19561 gttacggtca gtgttgtcga gtaaaacttc aggaatatga gctattccca gacttaaacc
    19621 ttttttctca cccataacaa tggcttcgtc actggtactg ttttccaatt tgtcaagcac
    19681 agaactgatc tgacttccaa ggaaggctga tacaatggca ggtggtgctt cgtttgctcc
    19741 cagacggtgt gcattctgag ctgtcatgat cgacgctttc agcaatgcgt tgtttttgta
    19801 aacagccatc aggatgttta caagaaaagt gatgaattgc aggttttctt ccggcgtttt
    19861 accaggtgtc agcaatccta cgccggtatc agtacccaaa gaccagttat tgtgttttcc
    19921 ggaaccgtta attccggcaa aaggtttttc gtgtaacagc aggcggaaac cgtgacgacg
    19981 tgccaccttt ttcataatag acatcaccag caggttttga tcgacagcca ggttgcattc
    20041 tccaaaaata ggagcaagct caaactgatt cggggcaact tcgttgtgac gcgttttcag
    20101 aggaattcca aatttgtatg cttcaaactc ca
//
LOCUS       DAOPYB010000034        19987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2812_length_19987_cov_9.973961, whole genome shotgun sequence.
ACCESSION   DAOPYB010000034 DAOPYB010000000
VERSION     DAOPYB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19987
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2812_length_19987_cov_9.973961"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PKH58_04365"
     CDS             <1..750
                     /locus_tag="PKH58_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNX88292.1"
                     /translation="DMEKPKLVFFFDEAHLLFNDMPQILLEKVEQIVRLIRSKGVGVY
                     FITQNPADIPERILGQLGNRVQHALRAYTPNDQKAVKVAANTFRPNPKFDTETAITEL
                     QTGEALVSFLDEKGAPNPVERAFILPPEGQVGPITPEERTHLIQSSLLYGIYEKDIDR
                     ISAFEIISEKMKATDLQKQQLEQQKQLEIEQREREKALREEEKRKRAEAKDDVLGGLL
                     KNVGRSVGRQVTSQLGRTITRTILGAFFGKK"
     gene            781..1557
                     /locus_tag="PKH58_04370"
     CDS             781..1557
                     /locus_tag="PKH58_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNX88293.1"
                     /translation="MKNRLFVFVLLMSSAFSVVAQYNAHMETTTDEQQIIRAQQAREF
                     IQSAKNLVFYLPFASQSTLDAKVADTKKIVWCENRHKEKDEAGIFTYSVNGIKAKPGA
                     YFDKDFTISVWVNVENVSKWSRIIDFGVGQNNSNVVFSNSASMSGIPGLAIYKGAGTD
                     LTQIRGTDKNRLKEGEWYHLAATLTGTVARLYINGVEIQKAENFYVPGNTARPMCWIG
                     ASNFEGESNFYGRMDELRIYNKALSDMEIKILADWKFQKP"
     gene            complement(1638..2750)
                     /locus_tag="PKH58_04375"
     CDS             complement(1638..2750)
                     /locus_tag="PKH58_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX88294.1"
                     /translation="MRVLFYILRKEFVQIFRDRMMLPMLLFMPFLQLLILPFAADMDV
                     RHLKIVIVDNDCSPSSRSLISKFTASDLFEIYAVSNSYPQALKSVETDKADIILQIPH
                     NFERDLIREGNVSVAVTADAINGVKAGLGTSYLSAIIQNFNRNIILEWSPDINLSAAQ
                     DIRYSFWYNPNLRYKNFIVPAILVLLVTVISGFISALNIVSEKEKGTIEQINVTPLKK
                     WQFIIGKLLPFWFIGLFDFSLGLLILHFVYNIPIVGSLSTLYLFAVVYITGILGFGLL
                     ISTVSKTQQQSMFVAFFFIMIFILMSGLFTNVESMPPWAHTISSLIPVTHFMHAVRAI
                     VLKGSVFLDLWVDMVYIFCFDIVAISAAVINYHKTA"
     gene            complement(2747..3859)
                     /locus_tag="PKH58_04380"
     CDS             complement(2747..3859)
                     /locus_tag="PKH58_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX88295.1"
                     /translation="MMNNTIKQLAAFLQKEFLHVFRDKRTLLVMFGLPIAQILIFGFA
                     LTNEVKNARLLVVNPKASVESQKMIEKIDASSYFTVTHIVPSRKDLETYFRNNEAQCA
                     LILPDDFAESATKPVQLQIVADATDPNFAKTVVFYLSAIIGEFHQLQTAQSVPMKINS
                     EVRMLYNPSLNGSMNFVPGVIALIMMIVCTTLTAIAIVREKEMGTIETLLVSPLKPFL
                     LLISKAIPYAVLSLVNLSFILLLSNLVLDVPIRGSVVLMLTECFLYILSCLALGLMIS
                     NIASSQAVAMLISMAGILLPTLLLTGFLFPLENMPWIFQWLSNIIPARWFFIIVKSIM
                     LKGLGLKDIWMETSILIGMTVILLTISLKRFKIRLE"
     gene            complement(3856..4590)
                     /locus_tag="PKH58_04385"
     CDS             complement(3856..4590)
                     /locus_tag="PKH58_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX88296.1"
                     /translation="MYSITADKLTKKFGNFTAVDAITFDVKEGEIFGFLGANGAGKTT
                     AMRILCGLSYPTAGQARVAGFDVYKQQEEIKKSIGYMSQKFSLYENLTVSENIEFYGG
                     VYGLRRKEIRPRMRNMLETLGLAGEANKLVSSLPLGWKQKLAFSTAIFHEPRIVFLDE
                     PTSGVDPVTRRQFWEMIYEAASRGITVFVTTHYMDEAEYCNRISLMVDGRIDALDAPA
                     SLKKQFGVETMSDVFYALARGAKRNE"
     gene            complement(4677..5600)
                     /locus_tag="PKH58_04390"
     CDS             complement(4677..5600)
                     /locus_tag="PKH58_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX88297.1"
                     /translation="MVNYIELHNISKTYDKGKVLAVNNISLSVSEGEIFGLIGPDGSG
                     KTSIFRMLTTLIIPDSGSAMVLGYDVVKDYRKIRNEVGYMPGRFSLYPDLSVEENLRF
                     YATMFGTTIEENYHLIRDIYVQIEAFGKRKAGQLSGGMKQKLALCCALIHKPKVLFLD
                     EPTTGIDPVSRKELWEMLRKLKTENICIFVSTPYMDEALQCDRIALIQQGEILSIDTP
                     QAVVEQFDKYIYAVSSDDTYKLLKVLMQYPERLNSYPFGEFAHLVAVAELDEIQLGEF
                     LKNNGFNDIRINRIDATIEDCFIDLSRQTKE"
     gene            complement(5653..6561)
                     /locus_tag="PKH58_04395"
     CDS             complement(5653..6561)
                     /locus_tag="PKH58_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNX88298.1"
                     /translation="MKKKSIFGIALCLVIMTIPSCNPDEKKGDASGYFETVETIVSAK
                     GNGTILSLDVDEGMQLEAGKKVGQIDSTQIYLTIRQVQEQKGAVLSRKPDISSQTAIL
                     KEQLIQANRELKRVENLFAGQAATQKQLDDARSIVAVLNRQLNAQQTTLDKTAASVGK
                     ETNPLEMQISLLEDQLRKCRIINPVTGTVLVKYAEPSEMTTIGRPLYKIADLSTLILR
                     AYITNNQLGSLKLGQKVIVLTDLGKEKLSEYEGTLTWISSKAEFTPKTIQTRDERSNE
                     VYAVKIAVKNDGFLKIGMYADVKFAR"
     gene            complement(6614..7867)
                     /locus_tag="PKH58_04400"
     CDS             complement(6614..7867)
                     /locus_tag="PKH58_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX88299.1"
                     /translation="MKPFVFLILIALSVSTVKSQEISLSECQELARSNFPLIRQHDLI
                     THSENNAATALLRKYYPQIQVVGQATWQSDVTKIPITIPNLTIPELEKDQYKLYAEVS
                     QLVYDGGETDLQRKTIHANALVEQQKTEVEMYKLRDRINQTYFGILLMDEQLKQNEIL
                     QNDLQTALEKIKVMVRNGVSLATNEQNLEAELLNVKQLNSGILTGKQSLIKVMALLTG
                     KQITDQTKFIHPASVSLNTGTQRPELSLLDQQINNLGLQRKQLTAQLRPKLNLFAQGG
                     YGKPGLNMFTNSFEPYFIAGAKINWQFSRLYTLGKERTNIDVQQKSLDLQKEIFNLNN
                     SISVTQQSGEIQKLRQMLQTDDEIVALRNKIKQTSAVQLENGVITSADFIREANAENQ
                     ARQKKALHEMQLLLAEYSLMWMKGN"
     gene            complement(7864..8490)
                     /locus_tag="PKH58_04405"
     CDS             complement(7864..8490)
                     /locus_tag="PKH58_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX88300.1"
                     /translation="MKKEIDTQKDKSTEEKIKKAAEKIFLEKGFSASRTRDIAEEAGI
                     NLALLNYYFRSKKNLYNIIMMEKLDVFFNIIIEELKDDTIDLNEKMTELINKYTDLLI
                     REPRLPLFIMSEVQQNPDFFAKRMNIKLKVREALGNKTLLYGKSLEDDMQLLLSLLGV
                     TIFPFIIRHSMQVLFNMNDEQYNQILNQRRELIPKLMNELYNQLNADK"
     gene            complement(8660..10141)
                     /locus_tag="PKH58_04410"
     CDS             complement(8660..10141)
                     /locus_tag="PKH58_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNX88301.1"
                     /translation="MKIFTSTQIHELDKYTIENEPVSSIGLMERAADRLMQQFRKNWS
                     LNTEIVIFAGPGNNGGDGLALGRMMLQVGYEVQVILLHTGKLSAECRQNKERLQEYFP
                     DNISEQTSRFLPPETNREAVIIDALFGSGLSKPIEGIYRDAVEWINKQPNNVVAIDIP
                     SGLPGEACMSVADCVVGADFTYSLQFPKLSFLLPENEKFVGKWNLIDIQLHPKGIEKT
                     ETGMIYLDKTDIQQVLKARSRFSNKGNFGHTLIWAGKKGMAGAAVLVSKAALRSGAGL
                     VSVHSDEMNRIILQTTAPEAIFKTELDNIEAYNAFAFGPGLGTDSHTTEMFTELLKSL
                     PGPCVLDADALNIIALQPHLIDYIPVDSVLTPHPKEFERLFGKSVNSLERIRLAREKS
                     RELNVVIVLKGANTLIATPDGKLYFNSTGNPGMASGGMGDVLTGIIAGLMAQGYSSAD
                     SSILGVYLHGLSADQALFYQSEESLLASDVIAQLGSAFKLLRT"
     gene            complement(10231..11004)
                     /locus_tag="PKH58_04415"
     CDS             complement(10231..11004)
                     /locus_tag="PKH58_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNX88302.1"
                     /translation="MNSGCYSELKLLLDESVKRYNNTDFISDDPIQIPKRFTRREDIE
                     IAGFLTATIAWGNRKSIIKSAGQLMELMDNCPYDYVMNEGRNGLNERFVHRTFNTVDC
                     NFFIRALHDIYTNRGGLEGVFSKGFATERTVFGVLKHFRTVFLQTPHDRRSEKHLSDV
                     TSNSSAKRLNMFLRWMVRKDDNEVDFGLWHYIPASALMLPLDVHTGDVARALGMLTRK
                     QNDWKAVMEITDILRTFDADDPVKYDFALFGMGVNGHLE"
     gene            complement(11001..11594)
                     /gene="pnuC"
                     /locus_tag="PKH58_04420"
     CDS             complement(11001..11594)
                     /gene="pnuC"
                     /locus_tag="PKH58_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNX88303.1"
                     /translation="MKIIELVAVVFGILSVWFASRESIRVFPTGIINVLLFIYIFFIA
                     RLYANAGINVLYLITNIYGWYNWSRKIDDKNVLEISRTTARQNTYIILFAAVFYISVL
                     LLLRWYNKSDLTYVNSYVPWMDAMNSSLFLCATILMTKKKLENWIFWILGNVISIPIT
                     ISQGLYMTSFQYLVFLFLAISGYVGWKKKLQSIKVSE"
     gene            complement(11591..14572)
                     /locus_tag="PKH58_04425"
     CDS             complement(11591..14572)
                     /locus_tag="PKH58_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNX88304.1"
                     /translation="MNTNSIKKLYFKDTSFASLMRHRIYNILLYASKYDAFMLEEDGR
                     VDEQIFNEYVSLNLRYPPRFTLVASEEEANQALKERTYELIISMPSGDSINPFRWAKE
                     VKINHPDIPIVILTPFSRMVSQRIANEDLSAIDYVFSWLGNSDLLLAIVKLIEDRMNV
                     EEDVDSVGVQVILFVEDSIRLYSSILPHLYKYVFVQSRSFMTEALNDHEQMLRMRGRP
                     KIMLARNYEEAMRIYSRYKNNMLGVITDVSFMQNGVKNPRAGIELCRQIRKEDTYIPL
                     IVQSTSEDNHPLADSVNAAFVSKNSKTQLLELRQIITNNFGFGDFEFVNPVTGEVETR
                     VENLRGLQDIIFKISEDTLFYHVSQNNISRWLYSRAMFPLAEFLKNIHVDTKEKTDMN
                     RVRQIIFDAIVTYRKIKNRGIVAVFQRDRFDQYSNFARIGDGSMGGKGRGLAFLDSMI
                     KSNDSLEEFRDTQICIPRTVVLCTDNFTEFMEMNKLYPIALSDADDEVILQHFLKASL
                     PRRIIADLYAFLGAINSPLAVRSSSLLEDSHYQPFAGIYSTYMVPYDGSSKTNMLQMI
                     TEAVKAVYASVFYRSSKAYMTATKNVIDEEKMAVVLQETCGSYYGERFYPSFSGVARS
                     LNFYPLGSEKPEEGIVNIAMGLGKYIVDGGVSLRFSPYYPNNILQTSTMDMALRETQT
                     YFNALDAREKHFVPQVDDGFNLLKVRVQDADKDGTLKFMASTFNPRDQVIYDSVFEEG
                     RRVITFANILQHNVFPLAEILREVLRIAHAEMGRPIEIEFAVNLDYSPKPTHTFYLLQ
                     IRPIVDSKEMINEDIGAIQEENTIISCENAMGHGITNDIYDIVYVKPEAFNASKNQLI
                     MYEIEKLNKKLSQEDRHYVLVGPGRWGSSDVWLGIPVKWPHISNARVIVESGLSNYRI
                     DPSQGTHFFQNLTSFGVSYFTINPFQKDGAYDIDFLNAQPAEFESDYIRHIRFDKPIV
                     VKVDGRKNKGVIMKPQTEEVS"
     gene            14975..16309
                     /gene="gdhA"
                     /locus_tag="PKH58_04430"
     CDS             14975..16309
                     /gene="gdhA"
                     /locus_tag="PKH58_04430"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNX88305.1"
                     /translation="MNLEKLMQQLEAKHPGEQEYLQAVREVLMTIEEVYNEHPEFEKT
                     KIAERLVEPDRIFTFKVPWVDDRGEVQLNIGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNALTTLPMGGGKGGSDFNPRGKSDAEVMRFCQSFMLELWRHIGADTDV
                     PAGDIGVGGREIGYLYGMYRKLAQMNTGVLTGKNLEFGGSLIRPEATGFGALYFVKQM
                     LETAGHDIKGKTVAVSGFGNVAWGAIIKATEMGAKVVTISGPDGYIYDENGISGKKID
                     YLLELRATCNDVVAPYAEEFGAQFVPNRKPWEVKVDIALPCATQNELNGDDARNIINN
                     GTQCVAEVSNMGCTPEAIDLFIEHKIMYGPGKAVNAGGVATSGLEMTQNAMHLSWGAS
                     EVDSRLHQIMSDIHEQCVKYGKQPDGYINYMKGANVAGFLKVAKAMLAQGIV"
     gene            16547..17032
                     /locus_tag="PKH58_04435"
     CDS             16547..17032
                     /locus_tag="PKH58_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNX88306.1"
                     /translation="MINNFYQFKANSLQGKEIDMKSYEGNVVLVVNTASKCGLTPQYE
                     GLEKLHKEYKDKGLVILGFPCNQFANQEPGNAKDISENCLINYGVSFQMFSKIDVNGS
                     TAHPIYKYLKKELKGFPGNEIKWNFTKFLVDRNGNPYKRFSPLTKPGQLRKDIETLLN
                     K"
     gene            17044..17496
                     /locus_tag="PKH58_04440"
     CDS             17044..17496
                     /locus_tag="PKH58_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX88307.1"
                     /translation="MAYEQLKLENQLCFPFYAVSRLITRAYQDDLDALGITYPQYLVM
                     MVMWEFEELSVIEIADKLVLNTNTVTPLLKRMETMELINRIPSETDHRKVMVKLTEKG
                     KIMQEAAAIIPMKLVQKLKIDPNKTNIEEINKLKSQLNALIELLKSVE"
     gene            17632..>19987
                     /locus_tag="PKH58_04445"
     CDS             17632..>19987
                     /locus_tag="PKH58_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX88308.1"
                     /translation="MRKLLLLTTLIMITLSTFAQEGTIKGRVFNMKTNEPIEFATIQI
                     EGTTIGSTSDLDGNFIFTGIKPGFVKLIARSVGFETTVSSEIQVQGNQTTFVDIAVPE
                     AAIVIKEVVVRQNLNIKRIESPISVLSVGVQEIEKSAGANRDVSKVLQSLPGVGATDP
                     NRNDLIVRGGGPSENVFYLDGIEIPVINHFATQGSSGGAVGVINPDFVREINFYTGAF
                     PANRTNALSSVMEIKQKDGSKDRVHTKLSVGASDAALTLDGPIGKKSTFIVSARQSYL
                     QLLFKLIGLPFLPTYNDFQVKYKYQIDQKNELTFVGIGAIDNMTLNTELEKTGTEAQK
                     YLLSYLPIYKQWNYTVGLVYKHFGEKHIDNWVLSRNMLRNNNFKFKGNDDTLPKVNEN
                     LLSDYTSDEAENKLRFERDYPYLPVKLKIGAGLRYSHYTNETFRRLFFNNTENELNYQ
                     TNLNLFGYQAFAQASDEYLDNRLKLSVGVSLVGNNYNKNMQNPLNQFSPRLSVSYTLS
                     DKWDLNANFGRYAMQPAYTTLGFRYATGGPLVNQNENLKYVTSNQAIVGIEYRPAEKM
                     RLTVEGFYKQYQNYPLSVSDGLSIASKGTDYGQIGDEEIISTGKGRAYGVEVLYKIME
                     WKNLNITSTYTFFRSEFTDKNDIYRPSSWDTRHLLNLISSYKFGKSWNIAMRWRYVGG
                     APYSPIDFQKSGNRVAWNITNQPYIDYANFNSLRLKDAHQLDLRLDKEFYFKKWVLNL
                     YTDIQNVYNFQSQSIPIYTNKTPEGTIIPDPNGDITRQGLRVLET"
BASE COUNT         5897 a         4422 c         4066 g         5602 t
ORIGIN      
        1 gatatggaaa aaccgaaact cgtattcttt ttcgacgaag cgcatctgct tttcaacgat
       61 atgccacaaa ttttactcga aaaagtggaa caaattgttc gcctgatccg ctcaaaaggt
      121 gtgggagttt acttcatcac ccaaaacccc gccgatatac ccgagcgcat cctcggacaa
      181 cttggtaacc gtgtgcagca tgcgctgcgc gcttacacac ccaacgacca gaaagctgta
      241 aaagtggcag ccaacacctt ccgccccaat ccgaaattcg atactgaaac cgccattacc
      301 gaacttcaga caggtgaagc cctggtttcc ttcctcgatg aaaaaggcgc acccaatccg
      361 gtggaaagag cttttattct tcctcccgaa ggccaggtag gaccaatcac acccgaagaa
      421 cgaacccatc tgatacaatc gtccttgctg tatggcatct acgagaaaga tatcgaccgg
      481 atttcagcct tcgaaatcat ctccgaaaaa atgaaagcca ccgacctgca aaaacaacaa
      541 ttggaacagc aaaagcagct cgaaattgaa caacgcgaac gcgaaaaagc tttgcgcgaa
      601 gaagaaaaac gcaaacgtgc agaagcgaaa gatgatgttt tgggcggatt gctgaaaaac
      661 gtaggacgca gcgtggggcg acaggtaacc agtcagctgg gcagaaccat tacacgcacc
      721 attctgggag ctttcttcgg aaagaaatga cagaaattca ttaaccaaaa ttttatctgt
      781 atgaaaaacc gtttatttgt tttcgtgtta ctgatgtcat ccgctttctc agtggtggct
      841 cagtacaatg ctcacatgga aaccaccacc gacgagcaac aaattatccg ggcacaacaa
      901 gcccgggaat ttattcagtc tgcaaaaaat ctggtgtttt atcttccgtt tgccagtcag
      961 agcactctgg atgcaaaagt ggccgacaca aaaaagattg tgtggtgcga gaaccgacac
     1021 aaagagaaag atgaagccgg tatttttacc tacagtgtga atggtataaa agccaaaccc
     1081 ggagcatact tcgacaagga tttcaccatt tctgtatggg taaatgtgga aaatgtcagc
     1141 aaatggagca ggattattga tttcggtgtt ggtcagaaca attcgaatgt tgttttttca
     1201 aactcagctt ctatgagcgg cattccgggt ctggccatct acaagggagc aggaacggat
     1261 ctgacacaaa ttcgcggaac ggataaaaac cggctgaaag aaggcgaatg gtatcacctt
     1321 gccgctacac ttaccggtac ggttgcacgg ctatacatca atggggtgga aatccagaaa
     1381 gccgagaatt tctatgttcc cggaaatact gcgcgtccca tgtgctggat tggggccagt
     1441 aatttcgaag gtgaaagcaa tttctatggc aggatggatg aattgcgcat ctacaacaaa
     1501 gcgctgtcgg atatggaaat aaagatactc gccgactgga aatttcagaa gccgtaaaag
     1561 caaatatcat actaacttaa aaacggaggt tgtgattaat ccaacttccg ttttattttt
     1621 aacggtgtct gatgttttta agctgttttg tgatagttga tgaccgccgc tgaaatagcc
     1681 actatatcaa agcaaaagat ataaaccata tcgacccata aatccagaaa tacactgcct
     1741 ttgagaacga ttgctctcac cgcgtgcata aagtgagtta caggaataag gcttgaaata
     1801 gtatgagccc agggcggcat actttccaca ttggtaaaca agccactcat caatataaaa
     1861 atcattatga aaaagaaagc aacaaacatg gattgctgct gggttttgct aacagtggaa
     1921 atgagcagac caaaccccag aataccggtt atatacacta cggcgaacag atagagtgta
     1981 ctcaggctgc ctacaatagg gatattgtac acaaaatgta ggataagcag tcccagactg
     2041 aaatcaaaaa gaccgataaa ccagaaggga agcaattttc cgataataaa ctgccatttt
     2101 ttcaggggag tgacgttaat ttgctcgatg gtaccttttt ccttttcact gacgatattc
     2161 agagccgaga taaacccgct gataaccgtt accagcaaaa caagtatagc cggtactatg
     2221 aaatttttat acctgagatt aggattgtac caaaatgaat accgtatatc ctgagcagcc
     2281 gacagattga tatcgggcga ccactccagg atgatattcc tgttgaaatt ctgaataatc
     2341 gcgctgagat agctggttcc cagcccggct ttcacaccgt tgatggcatc ggctgtaaca
     2401 gccacactga cattgccttc acggattaag tcccgttcaa agttgtgtgg aatttgcaaa
     2461 ataatatccg ccttatctgt ttccacagat ttgagagcct gaggatacga attggaaacc
     2521 gcataaattt cgaacaggtc ggaagccgtg aattttgaaa taaggcttcg tgatgaggga
     2581 ctgcaatcat tgtccacaat gacaattttc agatgcctga catccatatc ggcagcaaaa
     2641 ggcagaataa gcagttgtaa aaagggcata aataacagca ttggcagcat catccggtcc
     2701 ctgaaaatct ggacgaattc ctttcgcaat atatagaata gcaccctcat tccagccgga
     2761 ttttaaatcg tttcaaactt atggtgagca ggataacagt cataccaatc agaatagatg
     2821 tttccatcca gatatccttc aggccaagtc ctttaagcat aatggatttc acgatgataa
     2881 aaaaccaccg ggcaggtata atattggaaa gccactggaa tatccagggc atattctcga
     2941 gcgggaaaag aaacccggtg agcaacaagg taggaagtaa aattccagcc atcgatatca
     3001 gcatggcaac tgcctgtgaa ctggcaatat tggatatcat cagtccaagt gccagacagg
     3061 aaagtatgta taaaaaacac tctgtcagca tcaaaaccac actcccgcga atgggaacat
     3121 cgagcaccag attactcagc agcaggatga acgaaagatt tacgagcgat aaaacagcat
     3181 agggaatggc tttggatata agcagcaaaa aaggtttcag cggcgatact agcaacgttt
     3241 caatggttcc catttctttt tcacgaacaa tggcgatggc ggtcagcgtg gtgcatacta
     3301 tcatcattat caaggcgata acaccgggca caaaattcat cgatccattc agggaaggat
     3361 tataaagcat tcgcacttcg gaatttattt tcatcggaac cgactgagca gtctgtaact
     3421 gatgaaattc accgatgatt gccgacaggt aaaaaaccac cgttttggca aaattcggat
     3481 ctgtagcgtc agccacaatt tgcagctgaa caggcttcgt tgccgattcg gcaaaatcat
     3541 cgggcaggat gagcgcgcac tgggcttcgt tgtttctgaa ataagtttcc aggtctttcc
     3601 gggacggaac aatatgagtg acggtaaaat atgaactggc atctattttt tcaatcatct
     3661 tctgactttc gacacttgct ttcggattaa caaccaacag acgggcattt ttcacctcgt
     3721 tggtcaatgc aaagccaaat atcagaattt gcgcgatggg cagtccgaac atgaccagca
     3781 aggttcgctt atcgcggaag acatgcagaa actctttctg caaaaaggct gccagttgtt
     3841 ttatcgtgtt attcatcatt cgtttcgttt tgctccgcgg gctaatgcgt aaaacacatc
     3901 actcatcgtc tccactccga actgtttttt caggctggca ggcgcatcaa gcgcgtcaat
     3961 ccttccgtca accatgagcg aaattcggtt gcaatactcc gcctcgtcca tgtagtgcgt
     4021 ggtaacaaac acggtaattc cccgcgaagc tgcctcgtaa atcatttccc agaactgccg
     4081 ccgggtgaca ggatcaactc cgctggtggg ttcgtcgaga aatacaatcc tgggttcgtg
     4141 aaaaatagcg gtggagaaag ccaacttctg tttccagcca agtggcaacg aactaaccag
     4201 tttattggcc tcgccggcaa gtccgagggt ttccagcata tttctcattc ttggtctgat
     4261 ttcctttcgg cgcaaaccgt aaactcctcc gtagaattca atattttcgc tgacagtcag
     4321 attttcatac aatgaaaatt tctgactcat gtacccaatg cttttcttga tttcttcctg
     4381 ttgcttatag acatcaaatc ccgctaccct ggcctgcccg gctgtgggat atgacaaacc
     4441 gcaaagaatt cgcatagcag tagtcttccc ggcgccattg gcaccgagaa aaccgaaaat
     4501 ttcaccttcc ttcacatcga aagtaatggc atcaacggct gtgaaatttc cgaatttttt
     4561 tgtcagttta tcggcagtga tggaatacat attattgttt tcagaaacac actaacttat
     4621 caatctacac attacacatt acacaattct ttcttttttc tgcattcatc cgtcactcac
     4681 tccttcgttt gcctgcttaa atcaatgaaa cagtcttcaa ttgtagcatc gatgcggttg
     4741 atgcgaatat cgttaaatcc gttatttttc agaaattccc caagctgaat ttcatcaagt
     4801 tcggcgacag ctacaagatg cgcaaattcc ccgaagggat agctgttgag ccgttcggga
     4861 tattgcatca atactttcag cagtttgtaa gtgtcatcgg aactcacggc gtaaatatat
     4921 ttatcaaact gttccacaac tgcctgaggt gtgtcaatac tcagtatttc accctgttgt
     4981 atcagggcga tgcggtcaca ttgcagggct tcatccatgt agggcgtgga aacaaaaatg
     5041 cagatatttt cagttttcag tttccggagc atttcccaca gttcttttcg cgatacagga
     5101 tcaataccgg ttgtcggttc atccagaaac agcacctttg gtttgtgtat cagcgcacag
     5161 catagcgcca gcttctgctt cataccaccc gagagctgac ctgcctttcg ctttccgaat
     5221 gcttcaatct gcacataaat atcgcggata aggtgataat tttcctcaat ggtggtgcca
     5281 aacatggtag cataaaatct aagattttct tccacgctca ggtcgggata caatgaaaac
     5341 cgccccggca tatagcccac ttcattcctg attttgcggt aatctttcac cacatcgtac
     5401 cctagaacca tcgcggaacc gctgtcggga ataatcagag ttgtcagcat tcggaaaatg
     5461 gatgtttttc cggatccgtc gggacctatc aatccgaaga tttcaccttc acttaccgac
     5521 aaactgatat tattcacagc aagtactttg cccttgtcgt aagtttttga gatattatgt
     5581 aattcgatat aattcacaat cctttttggc agaagttatt agttctccga atgaatattc
     5641 cgggtttgat gattacctgg caaacttcac atcggcatac atgcctatct ttaaaaagcc
     5701 atcgtttttt acggctattt tcacggcata cacttcgttg gaacgttcgt cgcgtgtctg
     5761 aatggttttg ggggtgaatt cggctttact gcttatccat gttagcgttc cttcgtattc
     5821 ggataacttt tccttgccca gatcggtcag tacgattacc ttttgcccca gtttcaggct
     5881 tcccagttgg ttgttggtga tgtatgcccg cagaataagt gttgacaaat cggcaatttt
     5941 ataaagcggt ctgccaatgg ttgtcatttc actgggttcg gcatatttga ccagtacagt
     6001 tccggttact ggattgataa ttcggcattt tcgaagctgg tcttcaagta gcgatatctg
     6061 catttcaagc ggatttgttt ccttacccac actggctgct gttttatcga gcgtggtctg
     6121 ctgcgcgttg agctgccggt tgagtactgc cactatcgac cgtgcatcat ccagttgctt
     6181 ttgggtggca gcctgtccgg caaaaaggtt ctccacgcgt ttcaattcgc ggttggcctg
     6241 tatgagttgt tctttcaaaa tggcagtctg cgaactgata tcgggtttgc ggctgagaac
     6301 cgctcccttt tgctcctgca cctgcctgat tgtcaaataa atctgcgtac tgtcaatctg
     6361 accaaccttt tttccggctt caagctgcat tccttcatcc acatcgagcg ataatattgt
     6421 gccgtttcct ttagccgata cgatagtttc caccgtttcg aaataaccgg aagcatcgcc
     6481 ttttttttcg tcaggattgc aggagggtat cgtcattatt accagacaga gagcaatccc
     6541 gaatattgat ttcttcttca tttgttaagt tgaataaata taattaaatc aacatgctga
     6601 atttcagcaa atttcaattc cccttcatcc acatcagact gtactcagcc agaagcaact
     6661 gcatttcgtg aagcgctttt ttctgacgcg cctggttttc ggcattggct tcacgtataa
     6721 aatctgcgct ggtgattact ccgttttcta gctgaactgc acttgtttgc ttgattttat
     6781 ttcgtaaagc cacaatttcg tcatccgttt gcagcatttg tctcagtttc tgtatttcac
     6841 ccgattgctg tgtcacacta atgctgttgt tcaggttgaa aatttctttt tgcagatcca
     6901 gcgatttctg ttgtacgtca atgttcgtac gttccttccc gagtgtgtag agtctcgaaa
     6961 actgccagtt aatcttggct cctgctataa aatagggttc aaaactattg gtaaacatat
     7021 ttagtccggg cttaccgtag cctccctgcg caaagaggtt cagtttcgga cgcagctgag
     7081 cagtaagctg tttacgctgc aatccgagat tgtttatttg ctgatccagt aaacttaatt
     7141 ccggtcgctg tgtaccggta ttcaaagaaa cagaagcagg atgtataaat tttgtttgat
     7201 cagtaatttg tttgccggtg agaagtgcca tcacttttat cagtgattgt ttacctgtga
     7261 gtattccgga gttgagttgt tttacattca gcagttctgc ttcgaggttt tgctcgttgg
     7321 tggcaagcga tacaccgttt ctcaccatca cctttatctt ttcgagagca gtctgaaggt
     7381 cattctgcag gatttcgttt tgcttgagct gctcatccat caataaaatg ccaaaatagg
     7441 tctggttaat ccggtcccga agtttataca tttccacttc agtcttttgt tgttcgacca
     7501 aagcatttgc atgtatcgtt ttacgctgta aatcggtttc accgccatca tataccagtt
     7561 gtgagacttc ggcatacaat ttatactgat ctttttccaa ttcggggata gtgaggtttg
     7621 gtatcgttat cggaattttt gtcacatccg attgccaggt agcttgtccc actacctgta
     7681 tttgcgggta atattttctc agcaaagccg tagcggcgtt gttttccgaa tgtgtaatta
     7741 aatcatgctg ccggatgagc ggaaaattgg accgcgccag ttcctgacac tcggaaaggc
     7801 taatttcttg tgactttaca gtagaaacag ataacgctat cagtatcaaa aagacaaatg
     7861 gtttcattta tctgcattta actgattata aagttcattc attaacttag gaatcaattc
     7921 gcgtctttga ttaagtatct gattgtactg ctcatcgttc atattaaaca acacttgcat
     7981 gctgtggcga ataataaacg ggaaaatagt aacaccaagt aaactaagca aaagctgcat
     8041 atcatcttcc agactttttc catagagtaa cgttttgttg cctaatgcct ctctgacttt
     8101 caactttata ttcattcttt ttgcaaaaaa atcgggattt tgctgtacct cactcatgat
     8161 aaaaaggggt aacctgggtt ccctgataag caaatcggtg tatttattta tcagttccgt
     8221 cattttttcg ttcaaatcaa ttgtatcatc tttcagttct tcaattatta tattgaaaaa
     8281 aacgtccagt ttctccatca taataatatt gtacagattt ttcttacttc tgaaataata
     8341 attcagcaaa gcaaggttaa taccagcctc ttcggcgata tcacgtgttc gtgatgcaga
     8401 aaaacctttt tcaagaaaga ttttttcggc agctttcttg attttttcct cagttgattt
     8461 gtctttctga gtgtcaattt cttttttcat aattctgtgt ttttttaaag tcatggtaca
     8521 aatatagaac tttttctaat acattaatct cttgttttaa ttttttgttt taatatttca
     8581 ataagacaat acagaaaaat gatattacat tgatattctg atcgtagcgt cttataagga
     8641 cgtctgtttt aaaatatttt tatgtccgga gcagcttaaa tgcacttccc agttgagcaa
     8701 taacatcaga agcaagtaaa ctttcttctg actgataaaa tagcgcctgg tcggctgata
     8761 aaccatgtaa atagacaccc agaatcgaac tatcggcaga tgaatatccc tgtgccataa
     8821 gtccggcgat tattccggtg agcacgtcgc ccatgccacc ggaagccatt cccggattac
     8881 ccgtgctgtt aaaataaagt ttcccgtcgg gtgtagcaat cagcgtattt gcacccttaa
     8941 gcacaattac aacattgagt tcccgtgatt tttcgcgagc aagacgaatg cgttccagtg
     9001 aatttactga cttaccaaaa agcctttcaa actctttggg atggggagta agaacactat
     9061 cgacaggaat ataatcaatc aaatgaggct gaagggcgat aatattgagt gcatcggcat
     9121 ctagcacaca aggtccgggc agggacttca acagttcggt gaacatttcg gtggtatggc
     9181 tgtcagtacc taatccgggc ccaaaagcaa aagcgttgta agcctcgata ttgtccagtt
     9241 ctgttttaaa aatagcctca ggagctgttg tttgaaggat gatgcggttc atttcatcac
     9301 tatgcacact taccagtccg gctccggagc gtaaagctgc tttcgaaacc agcacagcgg
     9361 ccccagccat ccccttttta cctgcccaaa tcagtgtatg accgaaattt cctttattcg
     9421 aaaaacgcga tcgggctttt aatacctgct gtatatcggt tttatccaga taaatcattc
     9481 ctgtttcagt cttttcaata ccctttggat gcaactgaat atcaatcaaa ttccattttc
     9541 ccacaaattt ctcattttca ggcaataaaa acgataactt cgggaactgt aaactgtaag
     9601 taaaatcagc tccaactaca cagtcggcaa ctgacatgca ggcttcaccc ggcaatccgg
     9661 agggaatatc tatggcaaca acgttgttgg gttgtttgtt tatccattca acggcatcac
     9721 ggtatatgcc ttctattggt tttgataatc ccgaaccaaa aagtgcatca atgattactg
     9781 cttccctgtt tgtttcggga ggtaaaaagc gggaagtttg ttcggatata ttatccggga
     9841 agtactcctg tagtctttcc ttgttctgcc ggcattcggc cgataatttt cctgtatgta
     9901 gtaaaattac ctgaacttca tagccaacct gaagcatcat tcgtcctaat gccagtccgt
     9961 cgccgccatt atttccggga cctgcaaata tcacaatttc ggtatttaaa ctccagtttt
    10021 tcctgaattg ctgcataagc cggtcggcag cccgttccat tagaccgatg gagctaacag
    10081 gctcgttctc gatagtgtat ttatcgagtt catgaatttg agttgatgta aaaattttca
    10141 tagcaagatt ttaatacccc agcgaggcgt gtctgtttga agttatactg tttgtatttg
    10201 ttagagtgac ttgaccggaa ttatttgagg ttattccaaa tgaccgttaa cccccattcc
    10261 aaaaagagca aagtcgtatt ttaccggatc atcagcatca aatgtacgta aaatatcagt
    10321 aatttccatc acagctttcc agtcattttg ctttcgggta agcataccaa gtgcccgtgc
    10381 tacatcgccg gtatgcacat cgagcggaag catgagtgca gatgccggga tatagtgcca
    10441 cagaccgaaa tccacttcgt tgtcgtcttt tcgcaccatc cacctgagga acatattcaa
    10501 ccgtttggct gaagaattgg aagttacatc agacagatgt ttttcggagc gtctatcgtg
    10561 cggggtttgc agaaacacgg tacgaaaatg cttcaaaacc ccgaaaacag tccgttcagt
    10621 tgcaaatcct ttggaaaata caccttcaag acctccccgg ttggtgtaaa tatcatgcaa
    10681 tgcgcggata aaaaagttac agtcaacagt attaaaagta cgatgaacaa accgctcatt
    10741 taatccattc cggccttcat tcattacata atcgtacggg cagttgtcca tcagttccat
    10801 cagctgaccg gcgcttttga tgatgctttt ccggttgccc caggcaatag tggcagtcag
    10861 aaaaccggca atttcaatat cttccctgcg tgtaaaccgt ttgggtattt gtatgggatc
    10921 atcgcttata aaatccgtat tattataacg ttttacgctc tcatccagca gcaatttcaa
    10981 ttcagaataa caacctgagt tcattcgcta actttgattg attgtaattt tttcttccag
    11041 cctacgtatc ctgaaatagc taaaaaaaga aaaacaaggt actggaagct cgtcatgtac
    11101 aaaccctgtg aaatggtaat gggaattgaa atcacatttc ccaatatcca gaaaatccag
    11161 ttttccagtt tctttttggt catcagaatg gttgcacaga gaaacagtga actattcatt
    11221 gcatccatcc agggaacata cgaattgaca taggtcagat cggacttgtt gtaccagcga
    11281 agtaaaagca ggacagaaat ataaaatact gcagcaaaaa ggattatgta agtattttgc
    11341 cgggctgttg tacggctgat ttccagtaca tttttatcgt caatttttct cgaccagttg
    11401 taccagccgt aaatgttggt aatcaggtat aacacattga ttcccgcatt ggcatacaac
    11461 cgtgcaataa aaaatatata aataaacagc aatacattta taatgcctgt agggaaaacc
    11521 cggatactct ctctgcttgc aaaccaaacg ctgagaatgc caaacactac agcaacaagt
    11581 tcaatgattt tcatgacacc tcctctgttt gcggtttcat aattacccct ttgtttttac
    11641 gtccgtcaac ctttactact atcggtttat caaaccgtat atggcgaata taatccgatt
    11701 cgaattccgc gggctgagca ttcagaaaat caatatcgta agcaccatcc ttctggaatg
    11761 gatttatcgt aaaataggaa actccaaatg aggtcaggtt ctggaaaaag tgtgttccct
    11821 gactcggatc aatacggtag ttggacaatc cgctttctac tatcacccgt gcattgctga
    11881 tgtgaggcca tttgaccgga atgccgagcc acacatcact cgatccccac cgaccgggtc
    11941 ccaccagtac ataatgtcgg tcttcctgac tgagtttctt gttaagtttt tctatttcat
    12001 acattatcaa ctgattcttc gaggcattga aagcttccgg cttcacgtac acaatatcgt
    12061 aaatatcgtt ggttatacca tgacccatag cattttcgca actgataatg gtattttctt
    12121 cctgaatggc tccgatatct tcgttgatca tttctttgct gtccacgata gggcgaattt
    12181 gcagcagata aaaggtgtgt gtcggtttcg gtgaataatc cagattaacg gcaaattcaa
    12241 tctcaatcgg tcttcccatt tcagcgtgcg caatgcgtaa aacttcccgg aggatttcgg
    12301 caagcgggaa aacattgtgt tgaagaatat tggcaaatgt gattaccctg cgaccttctt
    12361 caaaaacact gtcgtatatc acctgatcgc ggggattgaa tgttgaagcc atgaacttca
    12421 gcgttccgtc cttatcggca tcctgtaccc ttactttcag caaattgaat ccatcatcca
    12481 cttgcggaac aaaatgtttc tcgcgggcat ccagcgcatt gaaatatgtt tgtgtctcgc
    12541 gcagcgccat atccatagtg cttgtctgca gaatattgtt gggataatag ggtgaaaacc
    12601 gcaaactaac gccaccatcc acaatgtatt ttcccaaacc catggcaata ttgactattc
    12661 cttcttcagg cttttcggag cccagcggat aaaagttcag tgaacgggca acaccggaaa
    12721 aagaaggata aaaccgctca ccataataac taccgcatgt ttcctgcaaa acgacagcca
    12781 ttttctcctc atcaatcaca ttcttggtgg cagtcatgta tgccttgctc gaccggtaaa
    12841 agacagatgc ataaaccgct ttcacagctt cggttatcat ctgcaacatg ttcgttttgc
    12901 ttgatccgtc gtagggaacc atatacgtcg aataaattcc cgcaaagggc tgatagtggg
    12961 agtcttccag caaacttgat gaacgcacgg caaggggtga attgattgct ccaagaaatg
    13021 catacaggtc ggcaataatc cgacgcggca aactggcttt gaggaaatgc tgaagaatga
    13081 cttcgtcatc ggcatcagac aacgcaatgg gataaagctt gttcatctcc atgaattccg
    13141 tgaagttatc ggtgcaaagt acaacggtac gaggaatgca aatctgggta tcgcggaatt
    13201 cttccaaact gtcgttggat ttaatcatcg aatcgagaaa ggcaagtccg cgtcccttac
    13261 cacccatgga tccatcgcct atccttgcaa aattcgaata ctggtcaaac ctgtcgcgct
    13321 gaaaaacagc aactatacca cggtttttaa ttttccgata agtcacaata gcatcaaaaa
    13381 taatctgacg caccctgttc atatctgttt tctctttggt gtccacatgt atgtttttca
    13441 gaaattctgc cagtggaaac atggctctgg aatataacca gcgcgaaata ttattctgcg
    13501 atacatgata aaaaagagtg tcttcagaaa ttttgaaaat aatatcctgc aggccccgca
    13561 ggttttcaac ccgcgtctcc acctcaccgg taaccggatt tacaaactca aagtcgccaa
    13621 agccgaaatt attggtaatt atctggcgca gttcgagcaa ctgtgttttg gagttcttgc
    13681 tgacaaaggc agcattaaca ctatcggcga gcggatgatt atcttcgctg gttgactgca
    13741 caatgagcgg aatataggta tcctctttgc gtatctgacg gcaaagttct attcccgcac
    13801 gaggattttt cacaccattc tgcataaaac ttacatcggt gattacaccc agcatattat
    13861 ttttataccg tgaataaatc ctcatggctt cttcgtaatt gcgtgcaagc ataatcttgg
    13921 gtcgtccgcg catacgaagc atctgctcat ggtcgttgag ggcttcggtc ataaacgaac
    13981 gcgactgcac aaaaacatat ttgtacaaat gcggcagaat ggatgaatac aaccggatag
    14041 aatcttctac aaaaagtata acctgtacac ctaccgaatc aacatcctct tccacattca
    14101 tcctgtcctc gataagtttt acgatagcca gcagcaggtc agagttgccc agccagctaa
    14161 acacatagtc aatagcgctc aggtcttcgt tggcaatacg ctgcgatacc attctcgaga
    14221 agggtgtcag tataacgata ggtatatccg gatggttaat tttcacttcc ttcgcccagc
    14281 ggaacggatt aatgctgtcg ccgctcggca tggaaataat cagctcatag gtacgttctt
    14341 ttaatgcctg atttgcttct tcttcgctgg ctaccagtgt gaaacgcggc ggataacgta
    14401 aattcagcga tacatattca ttaaatatct gttcgtccac acgtccgtct tcttccagca
    14461 taaatgcatc gtacttgctg gcatacagca aaatattgta tatacggtgg cgcatcagac
    14521 tggcaaatga cgtatcttta aaataaagtt tttttatgct gtttgtgttc atactttcaa
    14581 atttaaaatt ttcgcctgtc gaataaaaaa aatcaaccaa ttttttgcca ctcaaggaat
    14641 ttttccaaat caaatccgtt tgaagtaatt attcatactt gaaaacagat ttttctgaaa
    14701 ataattctca aaaatacttc aaaaaatttg attatcacaa ataaaacatt taaacttgca
    14761 ccgtaatcta aatgtaagca agcaaaagtt cccatttgtc ataaacagtg tattttgtaa
    14821 attaaatttt gaaataatag cttttcacta tcataatgtt acaaattatc atttttcatc
    14881 aaattcaaac tgaaagcatt acattctgaa aaacacaaaa tctgaaattt atatttttca
    14941 aaaaaaaaca tataacccca ataatcctac aatcatgaat ctggagaaat taatgcagca
    15001 actcgaagcc aaacatccgg gtgaacagga atacctgcaa gctgtacgcg aagtactgat
    15061 gaccatcgag gaagtttata atgaacatcc cgaatttgaa aaaacaaaaa ttgccgagcg
    15121 tcttgttgag cccgaccgga tattcacttt caaagtaccc tgggtcgatg atcggggcga
    15181 agtacagcta aatatcggtt accgggtgca attcaacaat gcaattggtc cctacaaagg
    15241 cggattacgt tttcaccctt cggtcaatct ttccattctg aaattcctgg gattcgaaca
    15301 gatattcaaa aatgcattga ctaccctgcc tatgggtggt ggtaaaggcg gctcggactt
    15361 caatccgcgc ggaaaatcgg atgcggaagt catgcgcttc tgtcagtcat ttatgctcga
    15421 actctggcgt catatcggtg ctgataccga cgtacctgcc ggcgatatag gtgttggcgg
    15481 acgtgaaatt ggctacctct atggcatgta ccgcaaactg gcacagatga atacaggagt
    15541 attgacaggc aaaaatcttg aattcggagg ttcgctcatc cgtccggaag ccaccggttt
    15601 cggagcctta tattttgtaa aacaaatgct cgaaactgcc ggtcatgata ttaaaggaaa
    15661 aacagttgct gtatcaggat ttggcaatgt agcctgggga gccataatca aagctaccga
    15721 aatgggtgca aaagtcgtta ccatttcggg tccggatggt tatatctacg acgaaaatgg
    15781 tatcagcggc aaaaaaattg attatctgct cgaacttcgt gccacatgca acgatgtggt
    15841 tgcaccttac gccgaagaat tcggtgccca gtttgttccc aaccgcaaac catgggaagt
    15901 aaaagtggac attgccctgc cctgtgccac ccaaaacgaa ctcaatggcg atgatgcacg
    15961 aaatattatc aacaacggta cccagtgtgt tgccgaagtg tcgaacatgg gttgtacacc
    16021 cgaagccatc gacctcttta tcgaacataa aatcatgtac ggaccgggta aagctgtcaa
    16081 tgccggtggt gttgccactt cgggacttga aatgacgcag aatgccatgc acctgtcgtg
    16141 gggcgcttcc gaagtggaca gtaggcttca tcaaattatg agcgacatcc acgaacaatg
    16201 tgtaaaatac ggaaaacaac cggacggcta tatcaactac atgaaaggcg ccaatgtggc
    16261 aggtttcctc aaagtggcaa aagccatgct cgctcaggga attgtataaa ctactttttt
    16321 ctacaacata gggtttatat catgaaaatg gtttcataaa taatatgaaa ccattttttt
    16381 gtataaaaaa gttcgaaaac cccaatcaac aactaatcag cccctcaccc attcggttaa
    16441 cttctgttaa tttatatttt tattcatata tttatttgca tcgcgcacga tatattatta
    16501 tttttgtatc gcaaacgaca taaatatcaa ttctaataaa aaaaatatga taaataactt
    16561 ctatcaattc aaagcaaact ctttgcaagg caaagaaata gacatgaaat cgtatgaagg
    16621 gaatgttgta ttagtagtaa atacagccag caaatgtggc ctgaccccac agtacgaagg
    16681 acttgaaaaa ctgcacaagg agtacaagga caaaggactg gtaattctgg gcttcccatg
    16741 caaccagttt gccaaccagg aacccggtaa cgcgaaagat atttcggaaa actgtctgat
    16801 caactacggc gttagcttcc agatgttctc aaaaattgat gtaaatggtt ctaccgccca
    16861 ccctatctac aaatatctga aaaaagaact gaaaggtttt ccgggtaatg aaatcaaatg
    16921 gaatttcact aaatttctgg ttgatcgcaa cggcaatccg tacaaacgtt tttcacccct
    16981 gactaaaccc gggcaattac gtaaggatat tgagacttta ctgaacaaat aaataaaaag
    17041 aatatggcat acgaacagtt aaaactggaa aatcaacttt gttttccttt ctatgcggta
    17101 tcgagactga taacaagagc atatcaggat gatctggatg ctcttggaat aacctatccg
    17161 caatatctgg tgatgatggt gatgtgggaa ttcgaagaac tttcggtgat agaaattgcc
    17221 gataagctgg tattaaacac taatacagtc acaccactac tgaaaaggat ggaaaccatg
    17281 gaactgataa accgtattcc ttccgaaaca gatcaccgaa aagttatggt taaactgacc
    17341 gaaaagggca agataatgca ggaagcagca gctattattc cgatgaagtt ggttcaaaaa
    17401 ttaaagatag atccgaataa aaccaatatt gaagaaataa acaagttgaa gtcacaacta
    17461 aacgcattga ttgaactcct taaatcagtt gaataaaaat tttcaacaag caataaatta
    17521 tcagaaataa gaatattcta catgcaacaa ttgattacac aaatgaatat ttcgaaaaaa
    17581 acacattatc tttaacacac cttaaaccac aaataataaa aataacgaaa tatgagaaaa
    17641 ttacttctgc taacaacttt aataatgatt actctttcca cttttgctca ggaaggaacg
    17701 attaaagggc gggtattcaa tatgaaaacc aacgagccga tcgaatttgc cactatacag
    17761 attgaaggaa caaccatcgg atcaacatcc gaccttgatg gtaattttat tttcacagga
    17821 ataaaacccg gatttgtaaa gctgattgct agatctgtag gttttgaaac taccgtttca
    17881 tcagaaattc aggttcaggg caaccaaaca acttttgtcg atatagctgt acctgaggct
    17941 gcaattgtta ttaaggaagt agtggtacgt caaaatctga atataaaacg catcgaaagt
    18001 ccgatttcgg tactgtcggt tggcgttcag gagatcgaga aaagcgccgg agcaaaccgc
    18061 gacgtttcga aagtacttca gtcacttccg ggagttggtg ccactgatcc aaaccgcaac
    18121 gacctgattg tccgcggtgg cggcccatct gaaaatgttt tctatctgga tggcatagaa
    18181 atacccgtca tcaatcactt cgccactcag ggctcatccg gaggtgctgt cggtgttatt
    18241 aatcccgatt ttgtacgtga aatcaacttt tacacaggcg ctttccctgc caaccgtacc
    18301 aacgcattaa gttctgtaat ggaaatcaag caaaaagacg gcagcaaaga ccgtgtacat
    18361 accaaattat cagtgggtgc atcggatgcg gctctaaccc tcgacggacc aatcggtaag
    18421 aaatcaacat ttatagtttc tgcccgtcag tcctacctgc agctgctatt caaactgatt
    18481 ggactgccat tcctgcccac ttacaacgac tttcaggtga aatacaagta tcagattgac
    18541 cagaaaaacg agcttacatt cgtgggcatc ggagccattg acaacatgac attaaatacc
    18601 gaactcgaga aaacgggaac agaagcacaa aaatatctgt tgagttacct gcccatctac
    18661 aaacagtgga attatactgt aggtttggtt tacaaacatt ttggcgagaa acacattgac
    18721 aactgggtac tgagcagaaa tatgctgaga aacaacaact tcaagttcaa gggcaatgat
    18781 gacaccctgc ctaaagtaaa tgaaaatctg ttatcggatt atacatccga cgaagctgaa
    18841 aacaaactgc gttttgaacg cgattatcct tatctgccgg taaaacttaa aatcggagca
    18901 ggactgcgtt attcacatta caccaacgaa acattccggc gtctgttctt caacaatact
    18961 gaaaatgaac tgaactatca gaccaatctg aacttatttg gttatcaggc atttgcacaa
    19021 gcatcagacg aatacctgga caaccgcctg aaactttcgg taggcgtgag cctggtaggt
    19081 aacaactaca acaagaatat gcaaaatcct ctcaatcagt tttcgccccg tctgtcagtt
    19141 tcctacactt tatctgataa atgggatttg aatgccaact tcggacgata tgccatgcag
    19201 cctgcttata ccacacttgg ttttcgttat gccaccggag gtccgctggt taaccagaac
    19261 gaaaatctga aatacgttac atccaatcag gctatagttg gtatcgaata ccgtccggct
    19321 gagaaaatgc gtttgacagt agaaggattt tacaaacaat atcaaaacta tccactttcg
    19381 gtatcggatg gattaagcat agccagcaag ggtactgatt atggtcagat aggtgatgaa
    19441 gaaataattt caacgggaaa aggaagggct tacggagtgg aagttctcta caaaatcatg
    19501 gaatggaaga atctgaacat tacctccact tacactttct tcagaagtga atttaccgat
    19561 aaaaatgaca tatatcgtcc gtcttcatgg gacacgagac atctgctcaa cctgatttcg
    19621 agctataaat tcgggaaaag ctggaacata gccatgcgct ggagatatgt tggaggtgca
    19681 ccctactcgc caatcgattt tcagaaatca ggcaacaggg tagcctggaa cataactaac
    19741 caaccttaca tagattacgc caacttcaac tcgctgcggc tgaaagatgc acatcagctc
    19801 gacttgcgct tggacaagga gttttatttc aaaaaatggg tattgaattt gtacaccgac
    19861 attcagaatg tttacaattt tcagtcgcag agtattccca tttacaccaa caaaaccccg
    19921 gaagggacca tcattccgga cccgaatgga gacattaccc gtcagggact gcgcgtatta
    19981 gaaacct
//
LOCUS       DAOPYB010000035        19945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2818_length_19945_cov_10.309854, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000035 DAOPYB010000000
VERSION     DAOPYB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19945
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2818_length_19945_cov_10.309854"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..148)
                     /locus_tag="PKH58_04450"
     tRNA            complement(75..148)
                     /locus_tag="PKH58_04450"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Asn,seq:gtt)
     gene            242..1231
                     /locus_tag="PKH58_04455"
     CDS             242..1231
                     /locus_tag="PKH58_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNX88309.1"
                     /translation="MKKILGIGNALTDILFRIESDEILQQLNLKKGSMQLVDDEKSSQ
                     IMSLFATQQPIMATGGSASNTINAISKLGTPGGFIGKINQDEIGNFYYNDSLNNGVKP
                     HLMYSQTPSGHCTVLVSPDGERTLCTFLGAASELFQEDLNENIFGEYDVFHIEGYLVQ
                     NHDLIHTALKMAKEANMIVSIDLASFNIVEENREFLREMIEKYVDITFANEEEAVAFS
                     ETTPEDALDKLSSLCDIAVVKLGKQGSLIKRGESLIRVGAKPANCIDTTGAGDMYAAG
                     FLHGLTQNYSLDVCGKIGSLVAANVVEVIGAKVSDNTWSEIYAEIDNILKDEN"
     gene            complement(1320..2699)
                     /locus_tag="PKH58_04460"
     CDS             complement(1320..2699)
                     /locus_tag="PKH58_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNX88310.1"
                     /translation="MELPFAESYKIKMVETIRRSTYAEREQWIREANYNLFALKSDQV
                     YIDLLTDSGTGAMSDRQWSALMLGDESYAGARSYYILRDAISDLLGFEHFLPTHQGRA
                     AENVLFSAIIKEGDVLPGNSHFDTTKGHIEYRKAFPVDCTIDEASDSQLEIPFKGNMD
                     LNKLEDVFRKYPKEKIPAVVLTITNNTAGGQPVSMENIRQTSFLCQKYGIPLLIDSAR
                     FAENAYFIKIREKGYENKTIREIVREIFSYADIMTMSSKKDAIVNMGGFLAMKSKDLW
                     KKCQMFCVMNEGFITYGGMSGRDMHALAQGLYEGTEFDYLETRIKQVEYLGMKLDEYG
                     IQYQRPAGGHAIFVDACKVLPNLPKEEFVAQTLGVELYLEAGIRGVEIGAILADRDPV
                     TRENRYPQLELLRLAIPRRTYTNNHMDVVAAALRNVYDRRNEIKHGYRITFENPIMRH
                     FTVELEKVE"
     gene            2821..3714
                     /locus_tag="PKH58_04465"
     CDS             2821..3714
                     /locus_tag="PKH58_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX88311.1"
                     /translation="MKLNKFKFKTYLYIVTAMICWSFSFIWTQTALESFQPITLITLR
                     LILASIFLYIFAKLTRKFKRLEKQDLKIFLLLAFFEPFLYYVGETYGLTLVEPTLASV
                     IVSTIPVFAPVFAFTFLREKISLLNFIGIAFSIFGVYLVVYQQNGSSATDIKGILLLL
                     LAVFSAICYSIILRKIPEKYSATNIVMYQSMLSLIYFIPTFLLVDFSAPVHGEISFAS
                     VKALLLLTIFASVMAFVLFADSVRVIGVARAQIFVNLIPVFTAIFSWILLREQLNLIQ
                     WLGIAVVIAGLFISQRRKAKV"
     gene            3734..4456
                     /locus_tag="PKH58_04470"
     CDS             3734..4456
                     /locus_tag="PKH58_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX88312.1"
                     /translation="MKVIFQVYLWIIAMPVLLLLTLITAILTLILSPVLPNSKLSYFP
                     ARWWSKIICLLCFVKVKTNGFDHLDRKQSYIFIANHQSIFDIFAVYGWFPFIFKWLMK
                     ANLRSIPFVGAACAAAGHIFIDRSNPVSARKSLLIAEKQLINGNSVFVFPEGTRTTNG
                     AMNKFKRGAFLMASDLHLPVVPVTLRGSFERHHKHSLRIIPGTITMTIHQPISPAELE
                     KEKSADIIRKTWDIINNTLTIS"
     gene            4697..5248
                     /locus_tag="PKH58_04475"
     CDS             4697..5248
                     /locus_tag="PKH58_04475"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNX88313.1"
                     /translation="MSDKYENLEKLKELLNKGIITEAEFNTEKEKILENANSASNDTT
                     TNTTPDNNHNFCALMHLSQFTNYLLPPLGLIAPLVMWLTRRNESNFVDVNGKIILNWT
                     LSVCIYSIIIVVGFLIFGAASIMTIINYENPIPLIGTIGIFLIPLIIFGILDLIFVIV
                     GAIKANNGEVWNYPLSIRFFKTK"
     gene            5286..5951
                     /locus_tag="PKH58_04480"
     CDS             5286..5951
                     /locus_tag="PKH58_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX88314.1"
                     /translation="MIKLDNINKTYYTEATSLHVLKGIDMSINNGEFVAITGASGSGK
                     STLLNILGILDNYDTGDYYLNNTLIKDLSEKQAAHYRNEMIGFVFQSFNLISFKNALE
                     NVALPLYYKNISRKKRNLIAMEYLDKMGLKDWAHHMPSEMSGGQKQRVAIARAIISQP
                     KILLADEPTGALDSQTSLEVMDVLKELNKTGITTIIVTHEHAVAETADRIIHIKDGLI
                     QLN"
     gene            5965..7218
                     /locus_tag="PKH58_04485"
     CDS             5965..7218
                     /locus_tag="PKH58_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX88315.1"
                     /translation="METIEEIISTLKHNKMRTVLTGLSVSWGIFILIVLLGAGNGLKN
                     GVTSNFSDRATNTVQMWPGRATKPYKGLKTNRQLNFTEKEIDNLKENVKESDEETGII
                     EKSVTVTYKTEFGNYELKGVDPSYAKIINLKFIPGDGRFINSLDVQNNSKVIVIDKKV
                     EENLFKGKPALGEYVKVGSVMFRVIGINSKKEQFAGTQVYIPFNTAQRIYNPDKKFWS
                     IAFTTKGLETKQANEDFDENLKKTMSQTMFFDPKDEQALWIRNAQRDYIETMNIFNGI
                     NIFVSIIGIFTLIAGIVGVSNIMLVSVKERTREIGIRKAIGAPPATILISIVIEAVLI
                     TALFGYIGMMMGIGLTEIVNFVMEKGASKNTSDMQMTVFKNPTVQLSYVFFSTTIIII
                     AGVIAGYMPARRATRIKPIEAMREE"
     gene            7319..8626
                     /locus_tag="PKH58_04490"
     CDS             7319..8626
                     /locus_tag="PKH58_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX88316.1"
                     /translation="MDNDQHAKTSRSGSWGAGLFDLDRWQEIWQTITHNKSRSVLTAF
                     GVFWGMFMLVAMVGAGQALKNGMMSGIEGFATNSCFVSNELTSEPYKGFKKGRSWFIE
                     NADIPLLENNVPEIQYVSPVLFGGRSDDKNVIRGEKSGSFNSKGVYPNYNLIDNNKLS
                     YGRFINDIDIEGKRKVCVIGERVYETLFNKGEDPVGKNIQLNGIYFQVIGVTRKTTNI
                     NIGGNAEETIILPFSTMQQAFNQGKRVHFLAITADKNIKVSVVEKRVKEELKKIHNIA
                     PTDETAVWSMNLEDMFTSFLYLGIGIGILIWVVGLGTLMAGGIGVSNIMLVTVRERTK
                     EIGIKRALGATPRNIITQIMTESVVLTLMAGVAGIMLGVGLLRVVGILLQNSDAFFKD
                     PQISFPVAIGALFILVIIGLFAGYLPARRAMQIKAIEAIREEN"
     gene            8747..9865
                     /locus_tag="PKH58_04495"
     CDS             8747..9865
                     /locus_tag="PKH58_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX88317.1"
                     /translation="MKKILKIALWSLLGIAVLATFYYLYKKSQPKIIKYQIEQVKSGT
                     VEKRTVATGKVEPRNEILIKPQMSGIISAVYKQAGDNVTAGDVIAKIKVIPDMVNLSS
                     AESRVSRAKISFDQSKTNFNRDRELFENKVISKEEFEKTQLQYNNDKEELKAAQDNLS
                     LVRDGISSSTSAYSNTLVRSTVTGTILDVPVKVGNSVIQSNNFNDGTTIATVANMNDM
                     LFVGKLDETEVGRVHVGMPMNITIGALQDHKLSAKLEYISPKGTEDNGAIMFEMKAAV
                     SVPSDLFIRAGYSANAEILLDEAKDVLTIPESCIEFSGDTAFVYVLKKTEPQEFTKKQ
                     VKLGLSDGVNIEIKGGLKVKEKIRGAEIIEKKKDKPQP"
     gene            9872..11176
                     /locus_tag="PKH58_04500"
     CDS             9872..11176
                     /locus_tag="PKH58_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX88318.1"
                     /translation="MKAKYLIIASLLLAAAQLPAQRIWTLEQCIDTALSNNRNIKQQQ
                     LSYKSKEISYSQAKANILPNLNASAGQNFNFGRSLGADNTYISSNSATSSFGISSDLT
                     LFDGLKMKYNIDAKKAELLAAGADWQKVEKDIILNVSTVFLQVLQNKELLKNAESQLE
                     LTRNNLIQRKELIKMGKLAEGEIYELQAQEAKEELQRVQAENNLQISKLDLSQVMDLE
                     DFQNLDVMVPANLMENELALLSAEEVYNSALLTRPELKAAQYRLQSSEKNIAIAKADY
                     LPSLGFGAQVGTGYYNMSRLPANESFNDQISNNLSTALGFRLSIPIFNRFEVKNRVKS
                     AKIEVENTKIEIEKTKIELRKTIQQAYYNAIAAKNRWESSQKSVRANEEAYRFASQKF
                     ESGRANQYEVNIAKNNLAQSLSEQTQAKYEYVFRMKLLELMR"
     gene            11243..11572
                     /locus_tag="PKH58_04505"
     CDS             11243..11572
                     /locus_tag="PKH58_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNX88319.1"
                     /translation="MNADNVKSQMRKGYLEYCILLILKKKPAYASDIITELKNVKLIV
                     VEGTLYPLLTRLKNSELLDYRWEESTQGPPRKYYEMTRKGEEFLQELENSWKEINDVI
                     FKIKESI"
     gene            11596..13284
                     /locus_tag="PKH58_04510"
     CDS             11596..13284
                     /locus_tag="PKH58_04510"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HNX88320.1"
                     /translation="MKKTLTINLNNTVFHIDDDAYELLQSYLAEVGVHFKSEAEKADI
                     MNDIEARIAELFTEKMQKNKNVITVEDVGEIIEVMGKPSQFVDEETDEENPQPEPKKA
                     AGKSKKFYRDTEHQLLGGVGAGLAAYFNMDITLVRVILIILLILTSGYLIPVYLLAWL
                     IAPKAETTAQKLEMRGEDVNIETIKNKMNDAKNYLESNEFKESATSVGQRFFEIFKAI
                     FKVIFAIIGSILAVVGVLLAAVLIFGLIVMLFEPDAITSIYPEFFTSLGGVTPEKIIL
                     MVVGLLLIIGCPVFALISWGIKATSKEQPKSYTAIWVSLVLWLAGIFMFLSVGTETVR
                     ILKENNWLTYHNGTFVLDNEQNFVTDSRNVADFQSIEASGAVQIELSQQENQEITVNS
                     LKEYIPNIKTEVVDGVLKVYSTDNLIKPRIKVRVGIDTITSINARGASNIEFITPFTV
                     KKLNLTLQGASKADMDFTSAQNLTFSLEGASRMDLKGYADTLLIDGAGATNVDAEDLK
                     TKVADIEVNGASKASVFATETFSGKAYGASRIVCKGNPRQRTNDTNTASKISYE"
     gene            13302..14075
                     /locus_tag="PKH58_04515"
     CDS             13302..14075
                     /locus_tag="PKH58_04515"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNX88321.1"
                     /translation="MKKFLFILSLIIGFGVQSFGQKNNSKDEKTDYEIITENRNLPEF
                     ENLNINGRFKVVVNQKSSQTVDVIAPNKYLAYVETVVEKNTLTIKMKEPKKEEDKNLF
                     DRLKTKYNDYLIRQPIEIHISVNNLKQIVLDGASVIETESEFEGKELYVNLSGASKGI
                     LQYTLSDLSVVLSGAAKLEMKGLTDNFQLDGSGASSYEGNGLKSRNVSVMMSGASKAE
                     VFATDSFDGKLTGASTVICSGNPKNVRQNSSRASKIVIK"
     gene            complement(14170..17019)
                     /gene="gcvP"
                     /locus_tag="PKH58_04520"
     CDS             complement(14170..17019)
                     /gene="gcvP"
                     /locus_tag="PKH58_04520"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNX88322.1"
                     /translation="MTDFLTNRHIGISEEDEKTMLQTIGVNSLDDLIEQTIPSNIRLT
                     EKIELPEPMTERKYSEHIVELGSKNKIYTSYIGQGWYDTCTPAVIQRNVFENPSWYTS
                     YTPYQAEISQGRLEALLNFQTAVTDFTGLPLANASLLDEATAAAEAATMAYNLRSRDK
                     VKAGANVLFVDENVFPQTLAVIRTRSFVQGIQLQVGDYSTVKFTDRHFGAIIQYPNAN
                     GSIENYTELVTDAHTHNCIVAVAADILSLALLTPPGEWGADIVFGSTQRWGIPMFYGG
                     PSAAYFATKEEYKRDMPGRIIGISKDANGKYALRMALQTREQHIKREKATSNICTAQA
                     LLASMAGFYTVYHGADGIREIAQRIHSMATYLNEMLGVYGYTQENENFFDTLKITLPE
                     NISRDDFKSLALDYEVNFNYYETGEIGLSLDETTELEDVNTLIEIFATAAGNTPVYAE
                     AEDVTGLKSFDENLERKSEFLTHEVFNKYHTETDMMRYIKKLERRDISLTHSMISLGS
                     CTMKLNAATEMLPLSRPEFSGIHPLAPADQTEGYAQLIANLEEYLSKITGFDACSLQP
                     NSGAAGEFSGLLVIKSYLLSKGEDKRNVLLIPASAHGTNPASAHQCGYEIVVVKCDEK
                     GNVDIADWNEKAAAHAQDLAGCMITYPSTHGIFEASIKDMCATIHKFGGLVYMDGANM
                     NAQVGLTNPGTIGADVCHLNLHKTFAIPHGGGGPGVGPICTTKQLSGFLPDKKFGKNT
                     VSAAPYGSAGVLAITYGYIRMLGEAGLEKSTKTAILNANYLAACLKDSYGVLYTGESG
                     RVGHELILECRNFKATSGVTETDIAKRLMDYGYHAPTLSFPVHGTLMIEPTESESLAE
                     LEKFTDAMLKIYAEIKEIEEGIADKTDNVLMNAPHPEYEIVGEKWEHPYTRAKAAYPA
                     GWVAENKFWINVARVDNAFGDRNLVCTCRPTSDWE"
     gene            complement(17218..19944)
                     /gene="ppdK"
                     /locus_tag="PKH58_04525"
     CDS             complement(17218..19944)
                     /gene="ppdK"
                     /locus_tag="PKH58_04525"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNX88323.1"
                     /translation="MSDTKRVYTFGNGKAEGKADMKNLLGGKGANLAEMNLIGVPVPP
                     GFTITTDVCNEYFEKGKEQVVAELKPEVEQAMKGVEDLMKSKFGDVENPLLVSVRSGA
                     RASMPGMMDTILNLGLNDTVVEGLIRKTGNARFAWDSYRRFVQMYGDVVLGMKPVNKN
                     DIDPFEKIIEEVKEAKGVHLDTELGVEDLKDLVKKFKAAVLKQTGKEFPESAYEQLWG
                     AICAVFDSWMNDRAILYRKMEGIPAEWGTAVNVQAMVFGNMGDTSATGVCFSRDAATG
                     EDIFIGEYLINAQGEDVVAGIRTPQQITKEGSMRWAKLAGVSEDERAAKFPSMEEAMP
                     EIYKELDTLQTKLENHYKDMQDMEFTVQEGKLWFLQTRNGKRTGAAMVKIAMDMYHQG
                     MIDEKTAVMRIDPLKLDELLHPIFDKAALKNAKVLTRGLAASPGAATGRIVFHADDAA
                     EWAKDGEKVIMVRTETSPEDLAGMAVAQGILTARGGMTSHAAVVARGMGKCCVSGAGA
                     IKIDYSNKTAEVGDIILKEGDYISLNGSTGDVYLGEVATMEAELDADFAEIMSLSDKY
                     TKMKVRTNADTPHDALTARKFGATGIGLCRTEHMFFEGEKIKAMREMILSENAEGRRE
                     ALAKILPYQQEDFEGIFAAMEGCPVTVRLLDPPLHEFVPHDEKGQQEMADTMKVSLKK
                     IKQRVEDLHEQNPMLGHRGCRLGNTFPEITQMQTRAILGAALNLKKKGITAIPEIMVP
                     LTGILGEFLEQERIIRSTAAELFAEVGDSIDFKVGTMIEIPRAALTANRIASAAEFFS
                     FGTNDLTQMTFGYSRDDIASFLPIYLEKKILKFDPFQVLDQKGVGQLISMATEKGRSV
                     RPDLKVGICGEHGGEPSSVEFCHMVGLNYVSCSPFRVPIARLAAAQAAIKNA"
BASE COUNT         6009 a         4168 c         4171 g         5597 t
ORIGIN      
        1 tgataaatct ggcgaaacct agcgtaaaca aaaaacactt acacgtttta tggtgtaagt
       61 gtttgatttt gaagctctcc ctcttggact tgaaccaagg accctctgat taacagtcag
      121 atgctctaac cgactgagct aaggaagaat ttttagcaat aatttctcaa ttgcgagtgc
      181 aaaaataata ggttttcctg aaatatgcaa tatctttgca aaaaaaaatg cactaaaaac
      241 aatgaaaaaa attctaggaa ttggtaacgc tctgactgac atactttttc ggattgaaag
      301 cgatgagata ttgcaacagt taaatttgaa aaaagggagc atgcaactgg tggatgacga
      361 aaaatcatca caaatcatgt cgcttttcgc cacacaacaa cccatcatgg caaccggagg
      421 gtcggcatca aacacaataa acgctatctc aaaactggga acgcccggag gatttatcgg
      481 gaaaatcaat caggacgaga ttggaaattt ttattacaat gatagtctga ataacggcgt
      541 taagcctcat ctgatgtata gtcaaactcc atccggacat tgcacggtac ttgtttcgcc
      601 cgatggagaa agaacgcttt gcacctttct gggggcagcc agcgaacttt ttcaggaaga
      661 cctgaatgaa aatattttcg gagaatatga cgtttttcac atcgagggtt atctggtaca
      721 aaatcacgac ctgatacaca cagctcttaa aatggccaag gaagccaaca tgattgtttc
      781 catcgatctg gcaagtttca acattgtgga ggaaaacaga gaattcctcc gggaaatgat
      841 tgaaaaatac gtcgatataa catttgccaa cgaagaagaa gctgtcgctt tctcggaaac
      901 gacacccgaa gatgctctgg ataaactttc ttcgttgtgt gatattgctg ttgtgaaact
      961 tggcaaacag ggatcgctga taaaacgggg cgaaagtctt atacgggtcg gggcaaaacc
     1021 ggcaaattgt atcgatacaa cgggagctgg tgatatgtat gcagctggtt ttctgcacgg
     1081 actaacccaa aattattcac tggatgtatg tggcaagata ggctctctgg tggctgccaa
     1141 tgttgttgaa gtaataggag ctaaagtatc ggacaatacc tggtctgaaa tatatgccga
     1201 aattgacaat attcttaagg atgagaattg attggcatac tttatccgtt taaaaaattc
     1261 aaccgggaag cagagaacag tctgttgctt cccggttttt ttatttccgt cagaccggat
     1321 tattccactt tctcaagttc tacggtaaaa tgcctcatta tcggattttc aaatgtaatc
     1381 cggtaaccgt gtttaatttc attccggcga tcatacacat ttctgagtgc agcagcaacc
     1441 acatccatgt gattattggt gtacgtgcgg cggggaatgg ccagccggag caattccagt
     1501 tgtggataac gattttcgcg cgtaacaggg tctcggtcgg ccagaatggc tcctatctcc
     1561 acgccacgta ttccggcttc gagataaagt tcaacaccca gtgtctgtgc tacaaattct
     1621 tccttcggca aattgggtaa tactttacat gcgtccacaa aaatggcatg acctcctgcc
     1681 ggacgctgat actgaatgcc gtattcgtcg agcttcatgc ccaggtattc cacctgtttt
     1741 atccttgttt cgagataatc aaattcggtg ccttcgtaaa gtccctgcgc cagcgcgtgc
     1801 atatcgcgtc ccgacattcc tccgtaggta ataaacccct cgttcataac gcaaaacatc
     1861 tgacatttct tccaaagatc tttcgatttc attgccagaa aacctcccat attgacaata
     1921 gcatcttttt tggaagacat tgtcatgatg tcggcatacg agaaaatttc acggacaatt
     1981 tcgcggatgg ttttgttttc gtatcctttt tcacggattt taataaaata agcattttcg
     2041 gcaaaacggg cagaatcaat caacagaggt atcccatact tctggcagag gaatgatgtc
     2101 tgacggatat tttccatcga aaccggctga ccaccagcag tattgttggt aatagtaagc
     2161 acgacagccg gtattttttc tttcggatat tttctgaaca catcctccag cttattcaaa
     2221 tccatgtttc ctttgaaggg tatttcgagc tgggaatcgc tggcttcatc aatagtgcaa
     2281 tcgacaggaa aagctttacg gtattcaatg tgtcctttgg tagtatcaaa atgcgaatta
     2341 ccgggtaaaa catcaccttc tttaattatt gccgaaaaaa gcacattctc ggctgccctt
     2401 ccctgatgtg tgggtaaaaa atgctcgaat ccaagtaaat cggaaatagc atccctgagt
     2461 atataataag atcttgctcc cgcgtaactt tcatccccca gcatgagcgc cgaccactgc
     2521 ctgtcgctca tggctccggt tccgctgtcg gtaagtaaat cgatataaac ctgatcgctt
     2581 ttcagggcaa aaagattata gttagcttct cggatccatt gttcacgctc agcatatgta
     2641 ctgcgacgaa tggtttcaac catttttatt ttgtacgatt cggcaaaggg caattccatt
     2701 ttatttgtat attttttata tattaatgca aatgtagcta ttttgaaaaa tacttcaaaa
     2761 taaatgtgct aattttggtg ctcggattta aaaaattaat tttgtgtaaa atattaccga
     2821 atgaaattaa acaagttcaa atttaaaacc tatctgtata tcgtcactgc gatgatttgc
     2881 tggtcgtttt cgttcatctg gacgcagact gccctcgaat catttcaacc aatcactctg
     2941 attacccttc gactgatttt agcaagtatt tttctatata tttttgccaa actcacccga
     3001 aaattcaagc gacttgaaaa gcaagacctg aagatcttcc tacttctggc ttttttcgag
     3061 ccattcctat actatgtagg tgaaacctac ggacttacgc tggttgagcc gacacttgct
     3121 tctgtaattg tttcgaccat accggttttt gctccggtct ttgcatttac tttcctgcgt
     3181 gaaaagattt cgctgctcaa ttttatcggg atagcgtttt ctatttttgg cgtatatctg
     3241 gttgtttatc agcagaacgg aagttccgct actgatatta aagggatact gctacttctg
     3301 ctggctgttt tttcagctat ttgctattcc atcattctga gaaaaattcc ggaaaaatac
     3361 tctgccacaa atattgtaat gtatcagagc atgctaagtc taatctattt cattcccact
     3421 ttcctgctgg tcgatttttc cgctcccgtt cacggtgaaa tttcgtttgc gtctgtcaag
     3481 gctttgcttt tattaactat atttgcatca gtaatggctt ttgttttatt tgcagactcg
     3541 gtaagggtaa tcggagtagc ccgtgctcag attttcgtta acctgatacc ggtattcaca
     3601 gccatttttt cgtggatatt actgcgcgaa cagttaaatt tgatccagtg gctgggcata
     3661 gcggttgtga ttgcaggttt gtttatcagt cagcgacgca aagcaaaagt atgattacat
     3721 caatcagaag tatatgaaag taatctttca ggtttatctg tggatcattg caatgcccgt
     3781 attattgctg cttacattga ttacggcaat acttacgctc attctgtcac ctgtcttgcc
     3841 aaacagcaaa ctgtcttatt tcccagcccg gtggtggtca aaaataatct gtcttctctg
     3901 ttttgtaaaa gtaaaaacga acgggttcga ccatctagac cggaaacagt cctatatttt
     3961 tatagccaac catcagagca tatttgatat tttcgccgta tatggctggt tcccttttat
     4021 tttcaagtgg cttatgaaag ccaatttacg cagcataccg tttgtcgggg ctgcctgcgc
     4081 agcagccgga catatattta tcgaccgttc gaatccggta tctgctcgca agagtctcct
     4141 gattgccgaa aaacagctga tcaacggcaa ttcagttttt gtgtttcccg aaggcacacg
     4201 taccacgaac ggagcgatga acaaattcaa gcggggagcc tttctgatgg cttcagacct
     4261 ccatctgcca gtggttcccg ttacattaag aggcagtttc gaaaggcatc acaaacattc
     4321 gttgcggata atacccggca ccatcaccat gaccatccat caacccattt ctcccgctga
     4381 attagaaaaa gagaaatctg ctgatataat ccggaaaacc tgggatatca ttaacaacac
     4441 actcaccata agttaatctt tgttaacgtc aaatcttttt taatcgggtt attcgtctat
     4501 atatctgtat attgctaaat attttcatat aataccttgt atttacattg tactatgtat
     4561 tgcatagtat tataattaat catatatttg cagcgttaat acacagaagg cattaataat
     4621 cagctttctc atttttcaaa ataaaaacca attctacaaa cagtatattc atttatctaa
     4681 aaaaacaacc aatattatgt cggacaaata tgaaaatttg gagaaactga aagaacttct
     4741 gaataaagga ataattacag aagctgaatt caacacggag aaggaaaaga ttctcgaaaa
     4801 tgcaaacagt gcaagtaatg acaccactac gaacactacc cctgacaaca accacaattt
     4861 ttgcgcactg atgcatttgt cacagttcac taattacctg ttacctccgc tgggcctgat
     4921 agctcctctg gtgatgtggt taacccggcg caatgaaagt aattttgtgg atgtaaacgg
     4981 taaaattatt ctgaactgga cactcagtgt gtgcatttac agcattatta ttgttgtcgg
     5041 ttttctgata ttcggtgccg catccatcat gactattatc aactacgaaa atccgatacc
     5101 actgataggc actatcggaa tttttctcat tccgctgatt atttttggca ttctggatct
     5161 gatttttgta atcgtaggcg ctattaaggc aaacaatgga gaagtgtgga attatccgct
     5221 gagcattcgt tttttcaaaa ccaaataaaa aaaacaaaac gcaattttta ttcaaaacaa
     5281 gagaaatgat taaacttgat aacattaaca aaacctacta caccgaagca acatcgcttc
     5341 acgtgctgaa aggaatagat atgtcgataa ataatggtga atttgtagcc attaccggcg
     5401 catccggttc cggaaaatcc accttgctca acatacttgg cattcttgac aactatgata
     5461 ccggtgatta ttatctgaat aatacgctta taaaggattt atcagaaaaa caggcagctc
     5521 attaccggaa tgagatgatt ggttttgttt tccagtcgtt caacctgatt agcttcaaga
     5581 atgcgcttga gaatgtcgca cttccgctat attacaaaaa tatcagccgc aaaaaaagga
     5641 atctgattgc catggagtat ctggataaga tgggacttaa agattgggct catcatatgc
     5701 catcagaaat gtcgggcggc cagaaacaac gtgtagctat agccagagca attatttctc
     5761 agccaaaaat cctgcttgcc gatgaaccaa ccggagcgct cgacagtcag acctcgctgg
     5821 aagtaatgga tgtgctgaaa gaactcaaca aaaccggaat taccaccatc attgttactc
     5881 atgaacatgc cgttgccgaa actgccgacc gcatcattca tatcaaagat gggcttatcc
     5941 aattaaatta aagtagattt atcaatggaa acaattgaag aaattatctc cacactgaag
     6001 cacaacaaga tgcgaacggt gcttacagga ctttctgttt cttggggaat tttcattctg
     6061 atagtgctgc tgggtgctgg caacgggctg aaaaatggtg taaccagcaa cttcagcgac
     6121 cgtgcaacca acacagtaca aatgtggccg ggacgtgcca caaaaccata taaaggactg
     6181 aaaaccaaca gacaactgaa ttttaccgaa aaggaaattg ataatctgaa ggaaaatgtg
     6241 aaggaaagtg acgaagaaac cgggattatt gagaaatcgg taactgttac ctacaaaaca
     6301 gaatttggca actatgaact aaaaggggta gatccaagct atgcaaaaat tattaacctg
     6361 aaatttattc ccggcgatgg gcggtttatc aactcgctcg acgtgcagaa taacagcaag
     6421 gttattgtca tcgataaaaa agtggaagaa aaccttttca aagggaaacc ggccctgggt
     6481 gaatatgtaa aagtggggtc ggtcatgttc agggtaatcg gcatcaattc taaaaaggaa
     6541 caattcgctg gtacacaggt atatatacca tttaataccg cacagagaat ttacaacccg
     6601 gataaaaagt tctggtctat tgccttcact accaaaggtc tggaaaccaa acaggctaac
     6661 gaggattttg atgaaaatct gaagaaaacc atgtcgcaaa ctatgttttt tgatcccaaa
     6721 gacgaacagg cactgtggat acgtaatgcc cagcgcgact atatcgaaac catgaatatc
     6781 ttcaacggta tcaacatctt cgtaagtatc attggaattt ttacgctcat tgccggaatt
     6841 gttggagtta gcaatatcat gctggtgtct gtaaaagaaa gaacacggga aataggcata
     6901 cgtaaggcta ttggcgctcc gccggctacc attctgattt ccattgtcat agaagctgta
     6961 ctaattactg cgcttttcgg atatatcggg atgatgatgg gtataggact gacagaaata
     7021 gtcaactttg taatggaaaa aggggcatca aagaacacat ccgacatgca aatgactgtt
     7081 ttcaaaaatc ctactgttca gctaagctat gtgttctttt caacaacgat tataattatt
     7141 gccggtgtca ttgccggata tatgccggcg aggcgtgcca caagaattaa accgattgag
     7201 gccatgcggg aagaataaat aatgcctctt aggttccaac tcaaagcgtg ggtgaaactt
     7261 caaaagagca taacaacaga atagaataaa aatgaataca actaataata ataatacgat
     7321 ggataatgac caacacgcta aaacgtctcg ttcgggcagt tggggagcag gcttattcga
     7381 cctggatcgc tggcaggaaa tctggcaaac cataacccat aacaagtcca ggagtgtgct
     7441 caccgctttt ggcgtattct ggggcatgtt tatgctggtg gctatggtag gagccggtca
     7501 ggcgcttaaa aacggcatga tgtcgggcat cgaaggtttc gctaccaact cctgctttgt
     7561 aagcaacgaa ttaacatcag aaccctacaa ggggttcaaa aaaggtagaa gctggtttat
     7621 tgaaaatgcc gatattccct tactcgaaaa taatgttccc gagattcaat atgtatctcc
     7681 cgtgcttttt ggaggccgga gcgatgataa aaatgtaata cgtggtgaga aatctggttc
     7741 attcaattca aaaggggttt atcccaacta taacctgatt gacaataaca aacttagtta
     7801 cgggcggttt atcaatgaca tcgacattga aggaaaaaga aaggtatgtg ttatcggtga
     7861 gcgtgtttac gaaacacttt tcaataaggg agaagatcct gtgggtaaaa atattcagct
     7921 gaacggcata tattttcagg taatcggtgt tacccgcaaa acaaccaata tcaatatcgg
     7981 tggtaatgcc gaagaaacca ttattttacc attctcaacc atgcagcagg catttaatca
     8041 ggggaaaaga gttcattttc tcgcgattac agccgataaa aacatcaagg ttagtgtggt
     8101 ggaaaaaagg gtgaaagagg aacttaaaaa aatacacaat atcgctccta cagacgaaac
     8161 agctgtgtgg tcgatgaacc tggaagatat gtttacttcg tttctttatc tcggtatcgg
     8221 gattggcatt ctgatttggg tcgtcggact cggcacacta atggcaggag gtattggtgt
     8281 gagcaatatc atgctggtaa ccgtacgcga acgcaccaag gaaataggaa taaaacgtgc
     8341 acttggagct accccccgaa atattatcac tcaaatcatg accgaaagtg ttgttctcac
     8401 acttatggcc ggagtagcgg gtatcatgct gggagtgggt cttctgcgcg tagttggaat
     8461 cttgcttcaa aactcggatg cattcttcaa agatcctcaa atcagctttc ccgtggcaat
     8521 cggtgcattg tttatcctgg tgattatcgg gttgtttgca ggttatcttc ctgcgcgacg
     8581 ggcaatgcaa atcaaagcca ttgaggcaat cagagaagaa aactaattga tcaaacataa
     8641 ttttcttaaa ctaaacttga aataaatttt accccgataa aagggatcag ttctccctgc
     8701 aaatcgaaaa aaaatacata acaatttaaa tacaaattat ttacaaatga aaaaaatttt
     8761 aaaaatagcg ctttggtcgc tgttaggcat agcagtactt gctacattct attatttgta
     8821 caaaaaatca caacccaaga ttatcaaata ccagattgaa caggtaaaat cgggtacagt
     8881 agaaaaaaga acagttgcta ccggtaaagt ggagccacga aacgaaattc tgattaaacc
     8941 tcagatgtcg ggtattattt cggccgtgta caaacaggct ggcgataatg tcactgcagg
     9001 tgatgtaata gccaagatta aggtcattcc ggatatggtc aacctcagtt cagccgaatc
     9061 acgcgtatca cgtgcaaaaa tttccttcga tcagtcgaaa acaaatttta accgggatcg
     9121 tgaactgttt gaaaacaaag taatctcgaa ggaagaattt gaaaaaaccc agcttcagta
     9181 caacaacgac aaggaagagc tgaaagctgc acaggataat cttagcctcg ttcgtgacgg
     9241 tatttcgagc agcacctcgg cttacagcaa cacgctggtg cgttcgacag taaccggcac
     9301 catccttgat gttcccgtta aagtaggtaa ttccgttatt cagtccaata acttcaacga
     9361 cggaactacc attgccaccg ttgccaatat gaacgatatg ctttttgtcg gcaaactgga
     9421 tgaaactgaa gtgggacgtg tacacgtggg aatgcctatg aacattacca ttggtgcatt
     9481 acaggatcat aaactttcgg ctaaactcga atacatttca ccgaaaggaa ccgaagacaa
     9541 cggtgctatc atgttcgaga tgaaagcagc cgtaagtgta ccttctgacc tatttatcag
     9601 agcgggatac agtgccaatg cagaaattct gctcgacgaa gccaaagatg tactgactat
     9661 ccccgaaagt tgcatagaat tcagcggtga cactgctttt gtttatgtcc tgaagaaaac
     9721 cgaaccacag gaattcacta aaaaacaggt aaaacttggt ttgtcggacg gtgtaaatat
     9781 cgaaataaaa ggcggattga aagtcaagga aaaaatccgt ggcgcagaaa ttattgaaaa
     9841 gaaaaaagat aaaccgcaac cgtaacacac aatgaaagca aaatatttga taatagccag
     9901 cttactgctg gcagctgctc aacttcccgc ccagcgtatc tggacactgg aacaatgcat
     9961 tgacacggcg ctgtccaaca accgcaacat caagcaacaa caacttagtt acaaatcgaa
    10021 agaaatttct tattcgcagg caaaggcaaa tatacttcca aacctgaatg catcagcagg
    10081 acagaatttt aacttcggtc gttcactcgg agccgataat acatatatca gctccaattc
    10141 ggcaacttcg tcgtttggca tatcatccga tctgactttg tttgatggct tgaaaatgaa
    10201 atacaacatc gatgctaaaa aggcggaact gcttgctgcc ggtgccgact ggcaaaaagt
    10261 tgaaaaagac attattctaa acgtctctac agtttttctg caagtacttc agaataagga
    10321 actgctcaaa aatgctgaaa gtcagctcga actgacaaga aacaacctga tacagcgaaa
    10381 agaacttata aaaatgggca aactagcaga aggtgaaatc tatgaactac aggcacagga
    10441 agccaaagaa gaacttcagc gtgtacaggc tgaaaacaac cttcagatat ccaaactcga
    10501 cctgtcgcag gtaatggatc ttgaggactt ccagaatctc gatgtaatgg tacctgcaaa
    10561 tctgatggaa aatgaacttg cattgctttc tgctgaagaa gtgtataaca gtgctctgct
    10621 cacccgtccc gaactgaaag cagcacaata ccgtctgcaa agcagcgaga aaaacattgc
    10681 cattgccaaa gcagattatt taccatcgct gggttttgga gcacaggttg gaaccgggta
    10741 ttataacatg agccggctac cggctaacga atcgtttaat gatcagatat cgaacaacct
    10801 gagcaccgct ttgggcttca gattatcgat acccattttc aaccggtttg aagtaaaaaa
    10861 ccgggtaaaa agtgcaaaaa ttgaagtaga aaacacaaaa attgaaatcg agaaaacaaa
    10921 aattgaactc aggaaaacaa ttcagcaagc ttactataat gctattgctg ccaaaaaccg
    10981 ttgggaatcg agccagaagt ctgtcagggc aaacgaagag gcctatcgtt ttgccagcca
    11041 gaaatttgaa tcaggaagag ccaatcagta tgaggtaaat attgcaaaaa ataatctggc
    11101 acaatcatta tctgaacaaa ctcaggctaa gtacgaatat gttttcagaa tgaaactact
    11161 ggaactgatg agataaattt tcagataatt tttccggatt gcagacctaa aatctttatt
    11221 aacatctgat taaataaaac ttatgaatgc agataacgta aaatcacaaa tgcgaaaagg
    11281 gtatcttgaa tattgcattt tgcttattct gaaaaagaaa cctgcgtacg cctcggatat
    11341 cattaccgag ctgaagaatg tgaagttgat cgtggttgaa ggaaccttgt atcctctgct
    11401 tacccggctc aaaaacagcg aattgctcga ctaccgctgg gaagaatcaa ctcagggacc
    11461 gccgcggaaa tactatgaaa tgacccgaaa aggggaagaa tttctgcagg aactcgaaaa
    11521 ctcgtggaaa gagattaacg atgtaatatt taaaatcaag gaaagtattt aaataatttt
    11581 caacaaaata aagacatgaa aaagacatta acaatcaatt tgaataatac agtttttcat
    11641 attgacgatg atgcatatga attattgcag tcttacctgg cagaggtagg tgtacatttc
    11701 aaatcggaag cggagaaagc tgacattatg aatgacatcg aagcccggat agccgaactt
    11761 tttacggaga aaatgcaaaa gaacaagaat gtaattaccg ttgaagatgt aggtgaaatc
    11821 atagaggtaa tgggtaaacc cagccagttt gttgacgaag aaacagatga ggaaaatcct
    11881 cagcccgagc ccaaaaaagc agccggaaaa tcgaagaaat tttaccgcga taccgagcat
    11941 cagcttctgg gtggcgtagg tgcaggactt gcagcctatt tcaatatgga cattacgctg
    12001 gtaagggtaa tactgattat tttgcttatt ctcacatcgg ggtatcttat tcctgtatat
    12061 ctgctggcgt ggttgatagc gccaaaggcc gaaaccacag cccagaaact tgaaatgcgt
    12121 ggtgaagatg taaatatcga aaccattaaa aataaaatga acgatgctaa aaactatctc
    12181 gaaagtaatg aattcaaaga atctgctacc tcggtaggac aacgtttctt cgaaatattc
    12241 aaggcaattt tcaaggtaat ttttgccatc atcggctcca tacttgctgt tgtaggagtt
    12301 ttactggctg ccgtacttat tttcggatta atcgtcatgc tgtttgaacc ggatgcgata
    12361 acaagtatct accccgaatt ctttaccagt cttggtggtg taactccgga aaaaataata
    12421 ctgatggtcg ttggactgtt gcttatcatc ggatgcccgg ttttcgcgct catcagttgg
    12481 gggataaagg caacttcgaa agagcagcct aaatcttata cagctatttg ggtttcattg
    12541 gtattgtggc tcgccggtat ttttatgttc ctgagcgttg gtaccgaaac agtcagaata
    12601 ctcaaggaga acaactggtt aacctatcac aatggcacat ttgttttgga taatgaacag
    12661 aacttcgtta ctgatagccg taatgttgcc gattttcaat ccattgaagc atcgggtgct
    12721 gtacaaattg aattaagtca gcaagaaaac caggaaatta ccgttaattc actgaaagaa
    12781 tatattccaa acattaaaac tgaggttgtt gacggagtac tgaaagttta ttcgaccgac
    12841 aatttaataa aaccccgtat caaagtaagg gtgggaattg acaccatcac ctcaatcaat
    12901 gccagaggcg cttccaatat tgaatttatc actcccttta cggttaaaaa actgaattta
    12961 accttacagg gtgccagtaa agccgatatg gattttactt cggcacaaaa tttgacattc
    13021 agtctggagg gtgcaagcag aatggatttg aaaggttatg ctgacacttt actaattgat
    13081 ggcgcaggcg cgacaaatgt tgatgcggag gatttgaaaa caaaagtagc cgacattgaa
    13141 gtgaacggcg cgagtaaagc aagcgtattt gctacggaaa cattcagcgg taaagcctac
    13201 ggagcaagca gaattgtgtg caaaggtaat ccccgccaga gaaccaacga tacgaataca
    13261 gcttcaaaaa tttcttatga ataatacaaa ataattaaaa catgaaaaag tttcttttta
    13321 tcctgagtct tattattggt tttggggttc agtcatttgg tcagaaaaac aatagtaaag
    13381 acgaaaaaac cgactatgaa atcattaccg agaacaggaa tttacctgag tttgagaact
    13441 taaacattaa tggtcgtttt aaggttgttg tgaatcaaaa gtcatcacaa actgttgatg
    13501 taatagcccc caataagtat ctcgcgtatg tggaaactgt tgtggaaaaa aatacgctga
    13561 caataaaaat gaaagaaccc aaaaaagagg aggataaaaa cttatttgac cggctgaaaa
    13621 ccaaatacaa cgattacctg atccgtcaac cgattgaaat tcatattagt gtaaataacc
    13681 tgaagcaaat tgtgctcgac ggagcttctg ttatcgaaac cgaatccgaa tttgaaggta
    13741 aagaactata tgtcaatctt tccggagcat cgaaaggcat cctgcaatat acgctgagcg
    13801 atttgtcagt ggtattaagc ggagcggcaa agctggaaat gaaaggtctt accgataatt
    13861 ttcaactgga tggaagtggc gcttcgtcat acgaaggtaa tggcctgaag tcaagaaatg
    13921 tatcggtgat gatgagcgga gcaagcaaag ctgaagtttt tgcaacagac agttttgatg
    13981 gcaaacttac aggagcaagc acagtcatct gttcaggcaa tccgaaaaac gtacgtcaga
    14041 attcttcaag agcgtctaaa atagttatta aataatactc tttgcttttt aaaattaaat
    14101 ttgatgaaga aacgccgttc agacaagtac tgacggcgtt tcttttattt atgcaagatt
    14161 agatactgtt tattcccaat cggaagttgg tctgcaggtg caaaccagat tacggtcacc
    14221 gaaggcattg tcaacacggg ctacattaat ccagaactta ttttcggcca cccagccggc
    14281 agggtaagct gccttggcac gagtgtaagg atgctcccat ttttcgccta caatttcata
    14341 ttcgggatga ggcgcattca tcagtacatt gtctgtttta tccgctatgc cttcttctat
    14401 ttccttgatt tcagcataga ttttcagcat ggcatcggtg aatttttcga gttctgccag
    14461 cgactcactt tcagttggct ctatcatcag tgtgccatgt acggggaaag aaagtgtcgg
    14521 cgcatgatat ccgtaatcca tcagacgttt ggcaatatcc gtttcagtaa caccggaagt
    14581 ggctttgaaa ttacgacatt ccagaattag ctcatgccct acccttccgg attcaccggt
    14641 gtacagtact ccatagctat ccttcaggca tgctgccagg tagtttgcat tgagaatagc
    14701 ggttttagtt gatttttcca gacctgcttc accaagcatg cgaatgtagc cataggttat
    14761 tgccaacact ccggcactgc cgtaaggagc agccgagact gtattctttc cgaatttttt
    14821 atccggtaaa aatcctgaca gctgttttgt ggtacagatt ggtcccacac ccggaccacc
    14881 acctccgtga ggtatggcaa aagtcttatg taaattaaga tggcaaacat cagcgcctat
    14941 tgtgccggga ttggtcagtc caacctgagc attcatattg gctccatcca tgtaaacaag
    15001 tcctccgaat ttgtgaattg ttgcgcacat atctttaatg cttgcttcga aaattccatg
    15061 ggttgaagga taggtaatca tacaacctgc cagatcctga gcatgtgcag cagctttttc
    15121 attccagtcg gcaatatcca catttccttt ttcgtcgcac ttcacaacta ctatttcgta
    15181 tccgcactga tgagcactgg caggattggt accatgtgct gatgcaggaa taagcagtac
    15241 attgcgttta tcttcgcctt ttgagagtaa atatgatttg ataaccagca aaccggaaaa
    15301 ttctccggca gcacccgagt taggctgaag actgcatgcg tcaaatccgg tgatttttga
    15361 caggtattct tccagatttg ctatcagttg agcatatcct tcggtttggt cagcaggagc
    15421 aagcggatga ataccgctga attccggtcg gctcaacggg agcatttcgg ttgccgcatt
    15481 gagtttcatg gtacacgaac ccagtgaaat catggaatga gtgagagaaa tatcacgtcg
    15541 ttcaagtttc tttatataac gcatcatatc ggtttcggta tgatacttat tgaatacttc
    15601 atgagtaagg aattccgatt tgcgttccag attttcatcg aacgatttca atccggtaac
    15661 atcttctgct tcggcataaa caggagtatt tccggcagct gttgcaaata tctctatcag
    15721 cgtattaacg tcttccagct cggttgtttc gtccaggcta agaccaattt ctcctgtttc
    15781 gtaatagttg aaattgactt cataatccaa tgcaagcgat ttaaaatcat ctctgctgat
    15841 attttcaggc aatgtaattt tcagtgtatc aaagaaattt tcattttcct gtgtataacc
    15901 ataaacacca agcatttcgt tgagatatgt ggccatggaa tggatgcgct gcgctatttc
    15961 ccgaattcca tccgcaccgt gataaaccgt ataaaatccg gccatcgaag ccagtaaagc
    16021 ctgtgcagta caaatattcg atgtggcctt ttctcgttta atatgttgtt cacgagtttg
    16081 taaagccatt cggagcgcat attttccgtt tgcatctttg ctgataccaa tgatacgtcc
    16141 cggcatatcc cgtttgtatt cctctttggt agcaaaatag gcagctgaag ggccaccgta
    16201 aaacattgga attccccagc gttgtgtcga gccaaagacg atatctgccc cccactctcc
    16261 cggaggtgtg agtaacgcca gacttagaat atcagcagca acagcaacaa tacaattgtg
    16321 cgtatgagca tccgttacga gttcggtata attttcgata cttccatttg cgttgggata
    16381 ctgaatgatt gcgccaaaat gccggtcggt gaattttaca gtactataat cacctacctg
    16441 caattgaatt ccctgaacaa atgaacgtgt acgaataact gcaagtgttt gcggaaatac
    16501 attttcatct acaaaaagaa catttgcacc ggctttcacc ttatcgcggc tgcgcagatt
    16561 gtaggccatg gtggcagctt cggcggcagc agtagcttcg tcaagtagag aagcatttgc
    16621 aagaggaagt cccgtgaaat ctgttacagc tgtctggaag ttaagcaggg cttccagtcg
    16681 tccctgagat atctcggctt gataaggagt ataggaagtg taccacgaag gattttcaaa
    16741 aacatttcgc tggataactg ccggtgtaca ggtatcgtac caaccctgcc cgatgtagga
    16801 agtgtatatt ttgttttttg aacccagttc aacgatatgt tctgagtact tacgttctgt
    16861 cataggttcc ggaagttcaa ttttttcagt caaccggatg ttggaaggaa tggtttgttc
    16921 tatcaggtca tcgagtgaat ttactccgat ggtttgtaac atagtctttt catcttcttc
    16981 cgaaatgcct atgtgtctgt tagttagaaa atcggtcata gtattcttgg gggtttgaaa
    17041 ttaatcctca aagataatgc ttttttatcg taaatttaat gttgagcagg tgtgaaataa
    17101 tattttcttt gaactaacaa taatcaaaca gcaatcaaaa aggctgagaa ataaaaaaaa
    17161 ggaaagccga atcatttgtt gatccggctt tcccgatatt attaatgaca atttgtatta
    17221 tgcgtttttg attgcagcct gagcagcagc cagacgggca atcggcacac ggaacggtga
    17281 gcagctcaca tagttcaatc ctaccatgtg gcagaattca acagatgatg gttcaccccc
    17341 atgttctcca cagataccca ctttgaggtc aggacgtaca gaacggcctt tttcagtagc
    17401 catgctgatc aactgaccta cacctttctg atcaagcacc tggaatggat cgaatttcag
    17461 aattttcttt tccagataga ttggtaagaa ggaagcaata tcatcacgtg aataaccgaa
    17521 ggtcatttgt gtaaggtcat tagttccgaa tgagaagaac tcggcagccg atgcaattct
    17581 gttggcggta agtgctgcac gtggaatttc gatcatggta ccgaccttga aatcgatact
    17641 gtcaccaact tctgcaaaca attcagcagc agtcgaacgg ataattcttt cctgttcgag
    17701 gaattcgcca agaataccgg tgagaggaac cataatttca ggaatagctg tgatgccttt
    17761 tttcttcaaa ttcagtgctg ctccaagaat agctcgtgtc tgcatctgtg taatttcggg
    17821 gaatgtattt ccaagacggc aaccacggtg tccgagcatc ggattttgtt cgtggagatc
    17881 ttcaacacgc tgttttattt tcttcaacga aactttcatg gtatctgcca tttcctgctg
    17941 acctttttca tcgtgaggaa caaattcgtg caatggtgga tcgagcagac gaactgtaac
    18001 cgggcatcct tccatcgcag caaaaatacc ttcaaagtct tcctgctgat agggcagaat
    18061 ttttgccagt gcttcgcgac gaccttccgc attttcggag agaatcattt cacgcatggc
    18121 tttaattttt tcaccttcga agaacatgtg ttctgtgcgg caaagtccga taccggttgc
    18181 tccaaacttg cgtgcagtga gggcatcgtg tggtgtatct gcatttgtac gtactttcat
    18241 tttggtgtat ttatccgaca ggctcatgat ttcagcgaaa tcagcatcga gttcggcttc
    18301 cattgttgca acctcaccaa gatatacatc tccagtagag ccgttaagtg aaatataatc
    18361 accttctttc agaatgatat cacccacttc ggctgtttta ttggaataat ctatcttgat
    18421 ggcgccggca cctgagacac aacattttcc cataccgcgg gctaccacag cagcgtgtga
    18481 agtcataccg ccacgggcag taagaatacc ttgtgctaca gccataccag ccaagtcttc
    18541 aggagaagtt tcggtacgta ccataattac tttctcaccg tcctttgccc attcagcggc
    18601 atcgtcagcg tggaatacta ttcggcctgt agcagctccc ggtgatgcgg caagtcctct
    18661 tgtaagcact tttgcatttt tcaaagctgc tttatcaaat atcgggtgaa gcaattcatc
    18721 cagtttgagc ggatcaatac gcataacggc tgttttttcg tcgatcatac cctggtgata
    18781 catgtccatc gcgattttca ccatggctgc tccggtacgt ttaccgttac gggtttgcag
    18841 gaaccacaat ttgccttcct gaactgtaaa ttccatatcc tgcatatctt tgtagtgatt
    18901 ttcaagctta gtctgcaagg tatccagttc tttgtagatt tcaggcatag cttcttccat
    18961 ggatgggaat ttggctgctc tttcatcttc tgaaacacct gccagtttag cccagcgcat
    19021 cgaaccttct ttggtaattt gctgcggagt gcgaatacct gccaccacat cttctccctg
    19081 agcgttgatc aggtattctc cgataaagat atcttcacct gttgcggcat cacgtgagaa
    19141 acaaacaccg gtagccgatg tatcacccat gttaccgaac accattgcct gaacattaac
    19201 ggctgttccc cattctgcag gtattccttc catcttacga tacaggatag cacgatcgtt
    19261 catccaggag tcgaacacag cgcagatagc accccaaagt tgttcgtaag ctgattccgg
    19321 gaattcctta ccggtttgtt tcaatacagc tgctttgaat ttctttacca ggtccttcag
    19381 atcttcaacg cctaattcgg tgtcaagatg taccccttta gcttctttca cttcttcaat
    19441 gattttttcg aacggatcga tatcattttt atttaccggt ttcattccca ataccacgtc
    19501 gccatacatc tgtacaaaac ggcggtatga gtcccatgca aaccgtgcat ttccggtttt
    19561 acggataagt ccttcaacaa cggtgtcgtt cagccccagg ttaaggatgg tatccatcat
    19621 accgggcatg gaagcgcggg ctcctgaacg aactgaaact aacagcggat tttcaacatc
    19681 gccgaatttt gatttcatca agtcttcgac acctttcatt gcctgttcta cttcaggttt
    19741 gagttctgct acgacctgtt ctttaccttt ttcaaaatat tcattacaaa catcggtagt
    19801 aatggtaaat ccgggaggca ccggaacacc aattaaattc atttcggcaa gattggcacc
    19861 tttaccgcca agcaggtttt tcatatctgc tttaccttcg gcttttccgt tgccaaaggt
    19921 gtaaactctt tttgtgtcac tcata
//
LOCUS       DAOPYB010000036        19903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2828_length_19903_cov_9.371372, whole genome shotgun sequence.
ACCESSION   DAOPYB010000036 DAOPYB010000000
VERSION     DAOPYB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19903
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2828_length_19903_cov_9.371372"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /gene="rimO"
                     /locus_tag="PKH58_04530"
     CDS             <1..1171
                     /gene="rimO"
                     /locus_tag="PKH58_04530"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNX88324.1"
                     /translation="NPDGEVVIVNTCGFIGDAKEESINTILQLAERKKEQQNKLFVMG
                     CLSERYLKELQLEIPEVDKYYGKFNYTDILKELGQEYRADLRLERSLTTPPHFAYIKI
                     SEGCNRMCSYCAIPIITGRHRSRPIEDIEQEVKMLVDKGVKEFQIIAQDLSYYGIDRY
                     KEPKLAELTRRISDIEGVEWIRLHYAYPAHFPYDVLEVMRERENVCNYLDIALQHVSD
                     NMLKKMQRRFTSAQTYELIERLRAEVPGIHLRTTMMVGHPGETEEDFEELKKFVRWAR
                     FERLGAFAYSHEEGTYAYKNYEDDIPEEVKQRRLDELMEIQREIAAEINETKIGQTMK
                     VIIDRKEEEYYIGRTEFDSPEVDPEVLIPITTSGVKVGDFYQAKIKSATDFELFA"
     gene            1253..1543
                     /locus_tag="PKH58_04535"
     CDS             1253..1543
                     /locus_tag="PKH58_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX88325.1"
                     /translation="MNNKELISSLALKMNLPKTEINNMLQTFTQTCIEQLKEDNTVAF
                     QGFGSLEVRKKDERLSVHPVTKIRTLIPPKLVVCFKQSNVLKEKLNSQPGHE"
     gene            1536..3152
                     /locus_tag="PKH58_04540"
     CDS             1536..3152
                     /locus_tag="PKH58_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX88326.1"
                     /translation="MSKEKISMQEMIDLMVKNRDITKKSSEEFIRQLLTTIEDTLVEG
                     DSVKISGLGTFKPQWNEPRKSVDVNTGKEIVIDGYYKATFTPDTELKNLINEPFAHLE
                     AVEINEKKPEENVTEEETDNFKPATEPMRVFEEQAEEIKGILNELGALKTRKASEDRK
                     TETEEEINETEVNEKQLSALADEEFVNQIVEVDDFDNSQIDEETESQTIEMVGENIEA
                     EVTTEQSELNTMHKSEETSNDFDIIRVGGKIIYPAVNEMPFNSQFTPEIEQSDKSESE
                     TIVYSVIEEEVISETATPVISEENSGINTEPKNAELPDFGVAMPADIEPETGENNPIQ
                     RLQDEQKVENQEIASKPEVQPEQEAVPDESNYNYETEVTRKRPVWLYILLAVVLLVAV
                     AAASAWFFSPGIFDNLKGKPKAVAAETVQKTMINPVEPVVADSLTTQSAPTETAVNVF
                     ETPRQYTEYIATEVMKRGSMLSTFAKQHLGHPYFWVYIYEANSDIIKDPNNVPLGADI
                     KIPKMDPRLIDTNNPECLDYALRLSEKYLK"
     gene            3378..4373
                     /locus_tag="PKH58_04545"
     CDS             3378..4373
                     /locus_tag="PKH58_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX88327.1"
                     /translation="MNQMVDIKELNERIERQSAFVDAITMGMNKVIVGQKHLIESLLI
                     GLLSDGHILLEGVPGLAKTLAIKTLADLIKADFSRIQFTPDLLPADVIGTMIFSQKQE
                     EFNIRKGPVFANFVLADEINRAPAKVQSALLEAMQERQVTIGDKTFKLEEPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMLKVVINYPKKEEEKLIIRQNIIKEKPAVSPILSPKDIV
                     AARDVVRQVYIDEKIERYIIDIVFATRFPEEYGLDSLKDMISFGASPRASINLALAAR
                     AYAFIKRRGYVIPEDVRAVCFDVLRHRIGLSYEAEANSLTSEEIITDILNAVEVP"
     gene            4494..5360
                     /locus_tag="PKH58_04550"
     CDS             4494..5360
                     /locus_tag="PKH58_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNX88328.1"
                     /translation="METNDLLKKVRKIEIKTRGLSQNIFAGQYHTAFKGRGMTFAEVR
                     EYQYGDDVRTIDWNVTARFGHPYIKVFEEERELTVMLLVDVSGSRDFGTVNMLKKDII
                     TEVAATLAFSTIQNNDKVGVIFFSDKIEKFIPPKKGKKHVLHIIRELLVFKPSSNQTD
                     IGGALKYFTNAIKKHSTAFVISDFMDHGFDRTLQIANRKHDIAALQVYDIREAEIPNV
                     GLIKLKDAETGERIWVDTADKRLRTTYKHKWNERQLELQKTFKQSGVDSVTMSTDEDY
                     VRALMKLFRLRA"
     gene            5386..6363
                     /locus_tag="PKH58_04555"
     CDS             5386..6363
                     /locus_tag="PKH58_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HNX88329.1"
                     /translation="MRKQFRLYVLIIIALSAGFISAQPVVIKAKMDSTQLWIGNQTGF
                     TFEIVQSPSQKIIAPVFSDTIVGNLDIVEQAKSDTVKLSNEKIQVNIRYKVTSFKDSL
                     IYVPAFPFISGKDTAWSNSTSLKVVQPFVIDTAANSLTDIKPVYKPPFDWKSFLQKLL
                     LAILLIALAVILFILIRKFVMKKPVFQSATPEEIIPANVEALQLLDKIKEEKLWQKGR
                     VKEYHTQLTDVLRTYIEKVFNIKSMEMTSDEILRNSQFLKVDKPSAYEGLKQILTLAD
                     LVKFAKWDPVNGENELSLMNAYLFVNQTTVQEVINDEIKADNATEVENK"
     gene            6403..7386
                     /locus_tag="PKH58_04560"
     CDS             6403..7386
                     /locus_tag="PKH58_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX88330.1"
                     /translation="MTFLNPEYLFLLLLLVPMIIWYLKEIHKSDASLQISSVQPLKDL
                     PKSKRMKFRHLPFVLRLLAIAMLIIAIARPQSSNNWRTESTEGIDIMMALDISGTMQA
                     EDLRPSRLEAAKNVASEFILSRPNDNIGLVVFAGESFTQCPLTVDHNVLINLFSGVRY
                     GLIEDGTAIGLGLANAISRIKDSKAKSKVIILLTDGSNNRGDIAPITAAEIAKTFGIR
                     VYAIGVGSYGTISIPIQTPFGVRYQQEETQFDEKTLKDIADITGGRYFRATNTNKLRA
                     IYQEIDQLEKTKISVKEFSNKSETYFIFALLAFMFFFAEILIRNTWLRTMP"
     gene            7456..8499
                     /locus_tag="PKH58_04565"
     CDS             7456..8499
                     /locus_tag="PKH58_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX88331.1"
                     /translation="MLRFAHPEYLFLLLPAAFLVAVFVRAAILRKKNIRLWGNTTLLS
                     WLMPNVSYARPRWKFYMQLIALVLIIIVLAQPQFGTKEEKVRKKGIEVMIALDVSNSM
                     LAQDIQPNRLQKAKQILSQLIDKMEEDKVGMIVFAGDAYVQMPITSDYGSAKMFLSTI
                     NPQMVPRQGTAIGSAIDIAIKSFGQKSGASRAIIILTDGENHEDDAIAAAKLAGDNGI
                     KVYVVGMGTPEGAPIPVDETVSFKKDKEGNVVVSKLNEQMCSGIAQAGGGVYVRADNS
                     NAAQRTVSKQLDTLAKSDMDTKVFSEYNEQFQSFAFIALLILIIEFFVFARKNKWLSR
                     IRLFDVKEQKNNG"
     gene            8526..9230
                     /locus_tag="PKH58_04570"
     CDS             8526..9230
                     /locus_tag="PKH58_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX88332.1"
                     /translation="MMNVLKNTTILVVALLLPVAGIAQKESDEVRTGNRHYNKKKYTE
                     AEIEYRKALVKNNKSFEANYNLGNALFRQGKYNEAYQQYKTALPFAGDNKKKLASELH
                     NMGNTLIGENKIQEAIDSYKMALKNNPADNDTRYNLAYAQMLLKKQQQENKDNKNNKN
                     NNKDKQNKDKDQQQQPKNEPDKEKKQEQPQPQQPQMSKENAQQILEALQQDEKDTQDK
                     AKKAQVHGRKKADKDW"
     gene            9300..11126
                     /locus_tag="PKH58_04575"
     CDS             9300..11126
                     /locus_tag="PKH58_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNX88333.1"
                     /translation="MKRALIVLTSFLLIYNVLTAQDKVSFTASAPATVYMDTPFQLKY
                     SVNASARDLRAPDFQFFEILAGPFEEHSSSYQNINGKASSSVNVAYTYTLLPQKTGTF
                     KIPGANIEVDGEKVYSNGLTIKVLPADKAQSQGQGGASSSNAEQGTQRISNDNIFIRT
                     SVSKTNVYEQEAILVTYKLYTLLDVAQFTDMKFPDFNGFLKQEIEQSKNAQLSYENYN
                     GKNYGTVVLYQVLLFPQRTGEINIDKANFTAILRIQNRAQVRSIFDDFFDSYSNVKKN
                     LTAPGARIVVNALPPGKPASFTGAVGNFNLTSSISTTNLKANEAAIIKVVISGTGNMK
                     LLQNPQIKFPDGFEVYDPKADNKFGTNAKGVSGTKTIEYMFIPQHSGVFNIPSAELSY
                     FDLNDRIYKTLRTPAYHLNVAKGTGGESPIIGNYTNKEDVTQLAKDIRYIYTGKIKQK
                     SEENPLFGSMLFWMLYLIPFLLACILFVFFRKQIRENADISLVKTKKANRVAQKRLKL
                     AQKFLSEGKKSQFYEEVMKAVWTYLSDKLSIPVVSLNKENIEAEMTNRGIDKDLTDEF
                     TNILNTCEFASYAPNTGQQEMGNLYEVTIEAISKVEEYFKKK"
     gene            11128..11889
                     /locus_tag="PKH58_04580"
     CDS             11128..11889
                     /locus_tag="PKH58_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX88334.1"
                     /translation="MKRIYIVFLLISVLFGPTMSQTNRQNDQIALANQMYKQGQFTDA
                     ARIYEEELKKGVSAELYYNIGNSYYKANEIGHAILNYERALRIDPGYEDASYNLDIAE
                     SKIVDNVNTTPTFFIRKWTNSLIKSNTSNQWAVISVVCFVLMLALFFLFAFSRIRSRR
                     KYSFFASVFFGILCVISLSFSGIRKDQFINHHEAVIMSGTVAVKSSPDQSGTDLFQLH
                     EGTLVKVKSVLGGWSEITLENGAVGWVEESAIERI"
     gene            11957..12616
                     /locus_tag="PKH58_04585"
     CDS             11957..12616
                     /locus_tag="PKH58_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX88335.1"
                     /translation="MDFIQTLDSIDKQWLLVLNNDYPDFWDGLMYGISNKFTWIPFYI
                     ALIFILIKYLKRKSFLIILALIICILLADQISSGLIKDMVHRLRPSRNPEIENMVCLL
                     KGYRGGKYGFVSSHAANSFGLALLTSLFFKNRKYTISVLIWATLVSFSRIYLGVHYPG
                     DVIGGALVGAGVAFLVYLLFTKIHLENVPSNTSALNLKLPLWVMAASFIALIPYSLWI
                     Y"
     gene            13153..13593
                     /locus_tag="PKH58_04590"
     CDS             13153..13593
                     /locus_tag="PKH58_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88336.1"
                     /translation="MKKSLTVFTIFIAILFAASAKAQLSVTSYSIYALGISTDSRKPF
                     SAELKTFANTGFYDLGFELDLFHNFKTHEYHRFSVGLGLNSMPFNEVYLNEITLPLVL
                     EVFPLKNFKKLSLLVEFAPAMDVNVDDAGDFSFRHLWGIRYRFN"
     gene            13605..14387
                     /locus_tag="PKH58_04595"
     CDS             13605..14387
                     /locus_tag="PKH58_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HNX88337.1"
                     /translation="MNLRTVNVNRYIMPLREGGSLPALAEADDGFNYVVKFRGSGHGT
                     KMLISELIGGAVAKYLNLKIPELVFVQLDEAFGRSEGDEEIQDLLKASTGLNLGLHFL
                     SGALAYDPVTTKIDAALASKIVWMDAFLTNVDRTVKNTNMLMWHKELWLIDHGSTLYF
                     HHTWTNWEKQAVRPFPLIKDHVLLEFASQLHEADEECRDILNDEVLSEVVNLIPDEWA
                     HWNENGETPEIIREIYFKFLSKRRTQSEIFLNEALNARKILL"
     gene            14365..14748
                     /locus_tag="PKH58_04600"
     CDS             14365..14748
                     /locus_tag="PKH58_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HNX88338.1"
                     /translation="MRGKYFYEYAVIRLFPKVEREEFINIGIILFSKEAKYIGMKYRP
                     DIEQICRTATELDFDLLGNTLKSFSKICNGDQSGGKIAEMDIAERFRWLTAVRSTCIQ
                     TSRPHNGFTDNPEKKLDELFAELVM"
     gene            14778..16526
                     /locus_tag="PKH58_04605"
     CDS             14778..16526
                     /locus_tag="PKH58_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88339.1"
                     /translation="MPRTRRLPKSVFCLWFLFILIGQAACRPVDEQQPAENLVELDSM
                     AYRYPRYVYNVVNNLIKQNQQTGYDRAYTGLILSVAAENMGRSLPSDSMITATVNAFS
                     MRPEYNPGNYLRALIYQGITRFHTGIADNRAYLPIKEALILSETTPTDQKTLQIACYY
                     LGVIHTKNNNVNQAHEYFKKAFVIAENLKDSVVLFKTCREMYWNRMKALDFLTARTLL
                     ETLQSFSNVSPDQIRDIRNAEAIYYNSLKRYRTALKIDYNLLRTDIQNKDSSALLADY
                     YRISDNYKFMNQPDSALHYGELATRNIRDTTFYLNYYYYLNVAELASQLKMWDKSTAA
                     YQQVYRLMNKTINNQLNTQIMELERKYDVASAENRAIKLKNNNIVLRLVLIILTMGLI
                     ITLLIFRNRSRLQMEQQKSLLQEKKILEQKQLIAEANEKQMMLDKQLTERKLIEKQFV
                     IPIYRQISQRNNAIKNFLLDLQSNSYITKNPQILQKIENEYRDFVQTTRVTEPQFLTD
                     ELFSGLTGISTEDSGLFNESEKMMLAFMATGSDNQQMATLMNTSVESIRVRKSKLKKK
                     MQENKVKIPKKLEADM"
     gene            16801..17628
                     /locus_tag="PKH58_04610"
     CDS             16801..17628
                     /locus_tag="PKH58_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX88340.1"
                     /translation="MAHDINLTSQEWLNIIFEGKNKDYGAYRLRQDSSRRHLIAFISV
                     AVLTVLAITVPGISKELGIGGRERNREVIQLIDANNMTKPEVPEEHIKKQYQAPPPVE
                     LKSTMKFTAPVIKKDELVKEENEVKSQDEITESKVAISVADVKGTNEDTGIDIAILNE
                     HHVIVADTEPEKVFEYVEQPPSFPGGDAALMDYLNGNISYPTIAQELEIQGKVTCSFV
                     VGKDGSIQDVKVERGVDRSIDKEAVRVISSMPRWIPGRQGGNAVKVKYYLPVVFRLQ"
     gene            complement(17704..18459)
                     /gene="hisF"
                     /locus_tag="PKH58_04615"
     CDS             complement(17704..18459)
                     /gene="hisF"
                     /locus_tag="PKH58_04615"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNX88341.1"
                     /translation="MLAKRIIPCLDIKDGKTVKGINFLNITDVGDPVELGKRYAEMGA
                     DELVFLDITATNEKRKTLSELVTRIAGQINIPFTVGGGISSVEDVSVLLSCGADKISV
                     NTAAVRNPQLIKDLADNFGSQCVVLAVDTKCIEGEWYVFLNGGRVSTDIRTLDWVKQA
                     VALGAGEILLTSMNHDGTKDGFALDITQAVSESVNVPVIASGGAGTMQHFVDVFEAGK
                     ADAALAASIFHYQEIGIQELKHFLKDKGVEVRI"
     gene            complement(18543..19862)
                     /gene="mtaB"
                     /locus_tag="PKH58_04620"
     CDS             complement(18543..19862)
                     /gene="mtaB"
                     /locus_tag="PKH58_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNX88342.1"
                     /translation="MIDNTIFNNKKVVFHTLGCKLNFAETSAIGKQLEEEGFMKVRHG
                     EQADICIINTCSVTDTADHKCRQAISRLHRQHPNAKMIVTGCYAQLKPEEIATIEGVD
                     LVLGANEKFSIQNYLNNNHPESKGEILRADILKVKDFKPSVSYDDRTRYFLKVQDGCD
                     YYCTYCTIPLARGHSRSGTIAETVETAMIAIRAGAKEIVLTGVNTGEFGKHTGETFIG
                     LLQALDNIEADVRYRISSVEPNLLTDEIIQFVADSRHIMPHFHIPLQSGTNEILKLMK
                     RRYTRELFAEKIATIKRILPDAFIGVDVIVGVRGETDELFEQSFEFIQSLDFSQLHVF
                     TYSERANTKMLEIEHIVPVKERKRRSESLHHLSAGKTEAFYNSQSGKTVQVLWEAKKE
                     GEKMHGFTENYIRAERNYDKKLVNTVETVILGDWNEDKTALNATPLS"
BASE COUNT         6225 a         3994 c         4256 g         5428 t
ORIGIN      
        1 aaatccggac ggagaagttg taattgtgaa tacctgcgga tttattggcg atgcaaagga
       61 agaaagcatc aacaccattt tgcagctggc cgaacgcaaa aaagaacaac agaataagct
      121 ttttgtaatg ggatgcctgt cagaacggta tttgaaggag cttcagctcg aaattcccga
      181 agttgataaa tactacggaa aattcaatta tacggatatt ttaaaagaac tcgggcagga
      241 atatcgtgcc gatttgaggc tcgaacgttc gctgacaacg cctcctcatt ttgcttacat
      301 aaaaatatcg gaaggctgca accgcatgtg ttcctattgc gctattccca ttataacagg
      361 ccgtcatcgt tcccgcccga tagaagatat tgagcaggaa gtgaaaatgc tcgtagataa
      421 aggagtgaaa gaatttcaga ttattgcaca ggacttatcc tattatggca ttgacaggta
      481 taaggaacca aaacttgccg aactgacccg aagaatttcg gacattgaag gagtggaatg
      541 gatccggtta cattatgctt atccggcaca tttcccgtac gatgttctcg aagtgatgcg
      601 cgaacgcgaa aatgtttgta attatctcga tattgcacta cagcacgtga gcgataatat
      661 gctgaaaaag atgcagcgtc gttttacgtc agctcaaact tacgaattga ttgaacgttt
      721 acgggctgaa gttcctggta ttcatctgcg cacaaccatg atggttggac atccgggcga
      781 aacagaagaa gattttgagg aactgaaaaa atttgtccgg tgggcgcgtt tcgaacgttt
      841 gggtgcgttt gcctattcgc acgaggaagg aacttatgca tacaaaaatt atgaagatga
      901 cattcctgaa gaggtcaaac agcgccgttt ggatgaactg atggaaattc agcgcgaaat
      961 tgccgcggaa atcaatgaaa caaaaatcgg acaaacgatg aaagtaatca ttgaccgtaa
     1021 agaagaagaa tactacattg gccggactga gtttgattca cctgaggtgg atcctgaagt
     1081 attaataccg attacaacat ccggggtaaa agttggagat ttttatcagg cgaaaattaa
     1141 aagtgcgact gattttgagc tgtttgcctg atcagtatat tgctttattt atttgtaatt
     1201 aactatataa cgtttttcat tccttttttt agaagttcta acatctatca tcatgaataa
     1261 caaagaatta atttcaagtc tggcattgaa aatgaatctt ccgaagacgg aaatcaataa
     1321 catgcttcag acttttaccc aaacctgcat cgaacagttg aaagaggata atactgttgc
     1381 atttcagggt tttggttcac ttgaagttcg aaaaaaggat gagcgcctgt ccgtccatcc
     1441 ggtaacaaaa atccgtacac tcattccacc aaagctggtt gtgtgcttca aacagagcaa
     1501 tgtactgaaa gaaaaactta attcacagcc aggtcatgag taaagaaaaa atatcgatgc
     1561 aggaaatgat tgatttgatg gttaagaaca gagatattac caaaaaatca tccgaagagt
     1621 ttatcagaca gctgcttaca accattgagg atactctggt ggaaggtgat agtgtgaaaa
     1681 tttcaggttt gggtactttc aaaccacaat ggaacgaacc gcgcaaaagt gtggatgtga
     1741 acacaggtaa agaaattgta atcgatggat attataaagc aacttttact cccgatacag
     1801 agctgaaaaa tcttattaat gagccttttg cacatctcga ggctgttgaa atcaacgaaa
     1861 aaaaaccaga ggaaaatgta actgaagaag aaaccgataa tttcaaaccc gcaacggaac
     1921 cgatgcgtgt attcgaagaa caggcagagg aaattaaggg aatactgaat gaattgggtg
     1981 ctttgaaaac caggaaagcg tcagaagaca gaaaaacgga aactgaagaa gaaatcaacg
     2041 aaacagaagt aaatgaaaaa caactttcgg cgcttgctga cgaagaattt gtaaatcaga
     2101 tagttgaagt agatgatttt gataacagcc agattgacga ggaaacagaa agtcagacaa
     2161 tagagatggt tggtgaaaac attgaagcag aggtaacaac agaacaatca gagttaaata
     2221 ctatgcataa atccgaagaa acttcaaatg attttgatat tatccgtgta ggtgggaaaa
     2281 tcatctatcc agcggttaat gaaatgcctt ttaattcaca gtttactcct gaaattgaac
     2341 aatccgataa atcagaatct gaaacaattg tttattcagt tattgaagaa gaagtaattt
     2401 cagaaactgc aactcctgta atttccgaag agaattccgg tataaacacg gaaccgaaaa
     2461 atgccgaact acctgacttc ggtgttgcaa tgcctgccga tattgaacct gaaaccgggg
     2521 aaaacaaccc aattcagaga ttgcaggatg aacagaaggt agaaaatcag gaaatagctt
     2581 ctaagccgga agttcaacca gaacaggaag cagtgccgga cgaaagtaat tacaactatg
     2641 aaacggaagt aaccagaaaa cgtccggttt ggttgtacat actcctggca gtagtcttgt
     2701 tggtggctgt cgcagctgca tcagcgtggt tcttttcacc cggtattttt gataatctga
     2761 aaggtaagcc aaaagcagtc gcagccgaaa ctgttcagaa aaccatgata aatccggttg
     2821 aacctgtagt ggcagattcg ctgacaaccc aaagtgctcc gaccgaaacg gctgtaaatg
     2881 tatttgaaac gccccgccaa tacacggaat atattgcgac tgaggttatg aaaagaggta
     2941 gcatgctaag cacttttgcc aagcaacatc ttggtcatcc ttatttctgg gtctatattt
     3001 acgaagccaa cagcgatatt atcaaggacc cgaacaacgt gccactggga gcagatataa
     3061 aaattccaaa gatggatccg cggttgattg ataccaataa tccggagtgt cttgactatg
     3121 cattaagact ttcagaaaag tatctgaaat aacttcaatt aaaacacata aaaaaggtct
     3181 ttttactgac ctttttttta tgtctgttta tacggcaaaa atcaatacat tttttgcaaa
     3241 attaagcctt acagatactc agagttgaca tgattgttta catattctaa attcaggtta
     3301 atagatatta aatcagatat ttaacatttt aatatgttgt atctttgttc ggaaaaaaaa
     3361 caaaaacaat attatatatg aatcaaatgg ttgatattaa ggaacttaat gaacgtattg
     3421 aacgacaaag tgcgtttgtt gatgccatca cgatgggtat gaacaaagtt attgtggggc
     3481 agaaacatct gatagaatca ttgctgattg gtttgctttc cgatggacat attttgctcg
     3541 aaggtgttcc cggtttagca aaaacgttag ctatcaaaac cctggctgat ttgataaaag
     3601 ccgatttcag ccgaatacag tttactcctg acttgttgcc tgccgacgtt atcgggacaa
     3661 tgattttcag tcagaaacag gaagaattca atatccgtaa aggaccggtt tttgccaact
     3721 ttgtgctggc tgacgaaatc aaccgtgctc cggcaaaagt acagagcgcc ttgctcgaag
     3781 ccatgcagga acgtcaggtt acgattggcg acaaaacctt taagctcgaa gaaccatttc
     3841 tggtaatggc tacccaaaat ccgattgagc aggagggaac ctatccgctt cccgaagcac
     3901 aggttgaccg ttttatgctg aaggtggtta tcaattatcc gaagaaagag gaagaaaaac
     3961 tgattattcg tcagaatatt atcaaggaaa agccagccgt aagtccaata ttgtcaccaa
     4021 aagatattgt cgcagcccgc gatgtggttc gtcaggttta tatcgacgaa aaaattgaac
     4081 gttacatcat tgatattgtt ttcgctaccc gtttccccga agaatatggt ttggattccc
     4141 tgaaagatat gatttcattt ggtgcatctc ctcgtgcttc tatcaacctg gctctcgcag
     4201 cgcgtgccta tgcttttatc aaacgccgag gatacgttat tcctgaagat gtacgtgcag
     4261 tttgcttcga tgtattgcgt caccgtatcg ggctgagcta cgaagctgaa gccaatagcc
     4321 tgacatccga agagatcatt accgatattt tgaatgctgt agaagtgccc tgatcaaaca
     4381 aaggtcaagt cacagaaaac ccgatagtaa ttaaatatcg gcataaaaat gtactgtaac
     4441 tagtaataat tacagaaaag caatcaagat ttcgccggta aaagattttg tatatggaaa
     4501 ccaacgactt acttaaaaaa gttcgtaaaa tagagataaa aacgcgaggt ctatcgcaaa
     4561 atatatttgc aggtcagtac catacggcct tcaaaggacg cggtatgact tttgccgaag
     4621 tgcgcgaata tcaatatggt gacgatgtac gtacaatcga ctggaatgta acagcacgtt
     4681 tcggtcatcc ctatattaaa gtatttgaag aagaacgcga attgacggtt atgctgcttg
     4741 tcgatgtgtc gggcagccgt gatttcggaa cagtcaatat gctgaaaaag gacataatca
     4801 ccgaagtggc agctactctg gcattttcta ctattcaaaa caacgataag gtgggagtaa
     4861 tatttttttc agataaaatt gaaaagttca taccacccaa aaaagggaaa aaacacgtac
     4921 ttcatataat tcgtgaattg cttgttttca aaccatccag caatcaaacc gatatcggag
     4981 gtgcgctgaa gtatttcacc aatgccataa agaaacattc aacagctttt gtcatttccg
     5041 actttatgga tcatggtttc gaccgtacat tacagatagc aaaccgcaag catgatattg
     5101 ctgcattgca ggtttatgat atacgtgaag ccgagatacc gaatgtaggg cttatcaaac
     5161 ttaaagatgc cgaaaccgga gaacgaatct gggttgatac tgctgataaa cgcttgcgaa
     5221 cgacctataa acacaagtgg aacgaacggc aacttgaatt acagaaaact ttcaaacaat
     5281 ccggagtgga ctctgtaacc atgagtactg atgaagacta cgtacgtgca ttgatgaaac
     5341 tcttccgatt aagagcttaa ataaaagatg tttattcgaa taaatatgag aaaacagttt
     5401 cgtttatacg ttttgattat catagcttta tctgccggat ttatttcggc acagcctgtt
     5461 gttatcaaag caaaaatgga ttccacacag ctatggattg gcaatcagac tggatttacc
     5521 tttgaaattg tgcagtcgcc cagtcaaaaa attatcgcac ctgttttttc agacaccatt
     5581 gtcggtaatc tcgatatcgt agaacaggcg aaatcggata cagtaaaact ttccaatgaa
     5641 aaaatacagg taaatatccg ttacaaggta acatcgttta aggactcgct gatttatgtg
     5701 cctgcatttc catttatttc gggaaaagat accgcatggt ccaattccac ttcactcaag
     5761 gtggtacaac ctttcgttat tgatacggct gcgaattcac tgacagatat taaaccggtt
     5821 tataaaccgc cattcgactg gaaaagcttt ttacaaaaac ttcttttagc catcctgctt
     5881 atcgcgttgg cggttatatt attcatcctg attcgtaaat ttgtgatgaa aaaacctgta
     5941 tttcaatctg caacacccga agaaattatt ccggcaaacg ttgaagcact ccagctgctg
     6001 gataagataa aagaggaaaa attgtggcag aaaggaagag taaaggaata tcacactcaa
     6061 ctcacagatg tcctgcgcac ttatattgaa aaggtgttta atatcaagag tatggaaatg
     6121 acttcggatg aaattttgag aaattcacag tttctgaaag ttgataaacc atcggcatat
     6181 gagggattga agcaaattct aaccctggca gatcttgtga aatttgcaaa gtgggatccg
     6241 gtgaacggag aaaatgaatt aagcctgatg aatgcatatc tttttgtaaa tcagactact
     6301 gttcaagaag tgataaatga cgaaattaaa gctgataatg ctactgaagt tgaaaataaa
     6361 taaaactgaa acctgcgaac ggcaggtgaa cttaacgaaa aaatgacatt tctaaatcct
     6421 gaatatctat tcttgctcct tttactagtg ccaatgatta tctggtacct gaaggagata
     6481 cacaagtcgg atgcgagtct gcagatttcg tcggtacagc ctttaaaaga tttgccaaag
     6541 tcgaaacgga tgaaattcag acatttacca tttgttttga ggcttctggc catcgctatg
     6601 ctcattattg ccattgcccg cccgcaatca tcaaacaact ggcggacaga aagcactgaa
     6661 ggaattgata taatgatggc actggatatt tcaggaacga tgcaggcaga agaccttcgt
     6721 ccaagccgtc tggaagctgc aaagaatgtc gcatccgaat ttattctttc acgaccgaac
     6781 gataatatcg gattggtggt gtttgccggt gaaagtttta cccagtgtcc acttactgtt
     6841 gatcataatg tgctgataaa tttgttttcg ggcgtcagat acggattgat tgaagacgga
     6901 acagccatcg ggctggggct tgccaatgca atcagccgca taaaagacag taaagcaaaa
     6961 tcgaaggtaa tcattctgct caccgatggt tcgaacaacc gtggtgacat tgctcccata
     7021 acagcagcag aaattgccaa aactttcgga atcagagttt atgctattgg tgtaggatca
     7081 tatggaacca tcagcatacc tatacaaaca ccttttggtg tacgttatca gcaggaagaa
     7141 acccagtttg atgaaaaaac gctcaaagat attgcagata tcaccggcgg acggtatttc
     7201 agggcaacca atactaacaa gctgagagcc atttatcagg aaattgatca actcgaaaaa
     7261 acaaaaatca gtgtaaaaga gttcagtaac aagagtgaga cctattttat ttttgccttg
     7321 cttgcattta tgttcttttt tgccgaaata ctgatacgaa acacctggtt gagaaccatg
     7381 ccgtaatttt tatcttaaaa ttaatacgtg ttaatgcgtt taatagctta taaattgact
     7441 ttataaattc agataatgtt aagatttgca catcccgaat atctttttct gcttttacca
     7501 gctgcatttc tggtagctgt cttcgtgcgc gctgccatac tacggaaaaa gaatatcaga
     7561 ttgtggggaa atacaacctt gctgtcctgg ttgatgccaa atgtgtcgta tgcacgcccc
     7621 aggtggaaat tttatatgca actgatagcc cttgtgctga taataattgt tctggctcaa
     7681 ccacagttcg gaacaaagga agaaaaagtc aggaaaaaag ggattgaagt gatgatagcg
     7741 ctcgacgtat cgaactccat gctggctcag gatattcaac ccaaccgcct tcagaaagca
     7801 aaacaaattc tttcgcagct gattgataaa atggaggaag ataaggtggg catgattgtt
     7861 tttgcaggcg atgcatatgt gcagatgcct atcacgtccg attacggttc ggcaaaaatg
     7921 tttctgtcga ccatcaatcc ccagatggtg cctcgacagg gtactgccat tggatcggct
     7981 atcgatattg ctatcaaatc attcggacaa aaatcgggtg ccagcagggc aatcatcata
     8041 ctcactgatg gcgaaaatca tgaggacgat gccattgcgg cagcaaaact agccggggac
     8101 aacggaatta aagtctatgt agtaggaatg ggaacccccg aaggagcccc catacccgta
     8161 gatgaaactg tgagcttcaa aaaagataaa gaaggaaatg tggtcgtttc caaattaaat
     8221 gaacaaatgt gctcaggaat tgcacaggca ggtggtggcg tttatgtgcg ggctgacaac
     8281 agcaatgctg cacagcgaac tgtgagcaaa cagcttgata ctctggctaa atcggatatg
     8341 gataccaagg tgttctccga gtataacgag cagtttcagt cgtttgcatt catagcattg
     8401 ttgattttaa tcattgaatt ctttgtattt gcccgaaaga ataagtggct gagtcgtatc
     8461 cgcctgtttg atgttaagga acaaaaaaat aacggttagt gtagaaattt cttttttcat
     8521 aaaaaatgat gaatgtacta aaaaatacta cgatacttgt tgttgcgctg ctgctcccgg
     8581 tggcaggtat tgcccagaag gaaagtgatg aagtacgcac aggcaacaga cattacaaca
     8641 aaaagaaata caccgaagct gagattgagt accggaaagc gcttgtaaag aataataaat
     8701 cattcgaagc aaactacaac cttggtaacg cactgtttcg tcagggaaaa tataacgaag
     8761 cttaccagca atacaaaaca gctttgcctt ttgccggcga taataagaag aaactggcat
     8821 ccgaattaca caatatgggg aatacactta tcggtgaaaa taaaattcag gaagcaattg
     8881 attcctataa aatggcattg aaaaataatc cggctgataa cgatacccgt tacaatcttg
     8941 cttatgcgca gatgctcctg aaaaaacaac aacaggaaaa caaggataat aaaaataata
     9001 aaaataataa taaagataag cagaacaagg acaaagacca gcaacaacaa ccaaaaaacg
     9061 aaccggataa agaaaaaaaa caggaacaac ctcaaccaca gcagcctcag atgtctaaag
     9121 aaaacgctca gcagatttta gaagctttgc aacaggatga aaaggatact caggataagg
     9181 ctaaaaaagc acaggttcac ggtagaaaaa aggctgataa agattggtaa tttacagaaa
     9241 acaagcattt aatttataca taaatgatca ctactgagca agagggagag taatattcta
     9301 tgaaacgagc attaattgta ttaacatctt ttctgttaat ctataacgta ttaacggcac
     9361 aggataaggt aagttttact gcctcagcac ctgccaccgt atatatggat acaccttttc
     9421 agttaaaata ctcggtcaac gcatcggcac gtgacttgcg cgcacctgac tttcagtttt
     9481 tcgaaatact tgccggacca tttgaagagc atagctccag ttatcagaat ataaacggaa
     9541 aagcatcgtc gagtgttaat gttgcataca catacacctt gcttcctcaa aaaacaggta
     9601 ctttcaaaat tcccggagcc aatatagaag tagatggaga aaaagtttat tccaacggat
     9661 taacgattaa agtacttccg gccgataaag cacagtcgca gggacagggc ggagccagtt
     9721 cttcaaatgc agagcagggg acacagcgaa tatcgaacga taatattttc attcgcacca
     9781 gcgtatcgaa aacgaatgtg tatgaacagg aagcaattct ggtgacatac aaactctaca
     9841 cattgcttga tgtggctcag tttacagata tgaagtttcc cgactttaac ggattcctta
     9901 aacaggaaat agaacagtcc aaaaatgctc agctgtccta tgaaaattat aatggaaaaa
     9961 attatggtac ggtagtatta tatcaggtgc tgttatttcc acaacgaaca ggtgaaatca
    10021 atatagataa agcaaacttt accgctattc tgcgaattca gaacagggcg caggttcgca
    10081 gtattttcga tgatttcttt gattcctaca gcaatgttaa gaagaatctg acagcaccgg
    10141 gtgccagaat agtggtcaat gctttgcccc cgggtaaacc ggcatcgttc accggagcag
    10201 tcggtaactt taatctaact tcttccataa gtactaccaa tctgaaagcc aatgaggctg
    10261 ctattatcaa agtagtgatt tcgggaaccg gaaatatgaa gctgcttcag aacccccaga
    10321 taaaattccc cgatggcttt gaagtgtatg atccgaaggc tgacaataag ttcggaacga
    10381 atgcaaaagg tgtttcggga acgaaaacca tagagtacat gtttattcct caacattccg
    10441 gtgtatttaa tattccttcg gcagaattat cttatttcga tctgaacgac cggatctata
    10501 aaacgctcag aactcctgct taccacctca atgtggctaa aggtacggga ggcgagagtc
    10561 ctatcatagg caattatacc aacaaggaag atgttaccca actggccaaa gatatccgtt
    10621 acatttatac cggaaaaata aaacagaagt ctgaagaaaa tccgttgttc ggttccatgt
    10681 tgttctggat gctatatctc ataccatttc ttctcgcctg tatcctgttt gtcttcttta
    10741 gaaagcagat cagagaaaat gccgatatta gtctggtaaa aaccaagaag gcgaataggg
    10801 tggctcagaa gcgattgaaa ctagcacaga aattccttag cgaaggcaaa aagtcacagt
    10861 tctacgaaga ggtaatgaaa gcagtatgga cgtatctgtc ggataagctt tctattccgg
    10921 tagtatcact gaataaagaa aatattgagg cggaaatgac taaccgcggc atagacaaag
    10981 atttgacaga tgaatttacc aatatcctaa acacctgtga atttgcaagt tacgcgccca
    11041 atacaggaca acaggagatg ggcaatctgt acgaagttac catcgaggca atcagtaaag
    11101 tagaagaata ttttaagaaa aagtaatatg aaacggattt atatagtatt cttgctgatt
    11161 tcggttttgt ttggtccgac aatgtctcag accaatcgac aaaatgacca gattgcactg
    11221 gcaaaccaga tgtataagca gggtcagttt accgatgcag ctcgcattta cgaagaagaa
    11281 ctgaaaaagg gagtttcggc cgagttgtat tacaacattg gaaacagcta ctacaaagct
    11341 aacgagatag ggcatgcaat actcaattac gaacgtgccc tgcgcatcga tcccggttac
    11401 gaggatgctt cctataatct ggatattgcc gaaagcaaaa ttgttgacaa tgttaataca
    11461 acacctacct ttttcatcag gaaatggacc aattcactga tcaagtccaa tacatcaaat
    11521 cagtgggctg taattagtgt tgtgtgtttt gttctcatgc ttgctctctt ttttctgttt
    11581 gctttttccc gtatccggag ccgccgtaaa tactcatttt tcgcgtcagt cttcttcgga
    11641 attttatgtg tcatttcgct gagtttttca ggtatccgga aagaccagtt tataaaccac
    11701 cacgaagctg tcatcatgtc gggaactgtg gcagtaaaaa gttcacccga tcagagtggc
    11761 accgatcttt ttcagttgca tgaaggtaca ctggtgaaag tgaagagtgt tttaggtggc
    11821 tggtcagaaa tcaccctcga aaatggtgct gtgggatggg tggaagaaag tgctattgaa
    11881 aggatttgat aaagttacat ggtacaagct acaagtaaaa caaacagact aaacaccagt
    11941 cactaataga tgttaaatgg atttcattca gacactcgat agtattgaca aacagtggtt
    12001 gttggtgctg aacaacgact atcccgattt ttgggatgga ctgatgtatg gcatcagcaa
    12061 taaatttacc tggatacctt tctacattgc tcttattttt attctgatta aatacctgaa
    12121 gcgaaaatcc tttttgatta ttctcgcatt aattatttgt attctccttg ccgatcagat
    12181 ttcatccgga ctgattaagg atatggtaca ccggctgcgt ccctcacgaa atcccgaaat
    12241 agaaaacatg gtctgcctgc tgaaaggcta tcggggtggg aaatacggtt ttgtttcctc
    12301 tcatgccgcc aattccttcg gactggcttt gcttacctca ctgtttttca aaaaccgtaa
    12361 atataccata tcggtattaa tctgggcaac tctagtctcc ttttctcgta tttacctggg
    12421 cgttcattat cccggcgatg ttattggcgg agcattggta ggagcgggag tagcattcct
    12481 cgtgtacctg ctgtttacta aaatccatct ggaaaacgta ccatccaaca cttctgcact
    12541 caacctgaag ctaccccttt gggtaatggc agcctctttc attgcgctga tcccttacag
    12601 tttgtggatt tattaaccga taatatcact ttacctgcct ttttccatca ttaactgtca
    12661 cagtaaattg aataaaccca ttcgaaaatc aattattagt gcttaacatc gatttctgag
    12721 tgcatcacta gcattcctgt atgctcctta ttcatttctg caacataata tattttttaa
    12781 cattgttgtc cgatgaaaaa ctaagaaaac attataatat ggagataatt aagcaaatat
    12841 aggcattaag ccctgcaggg gcttacttat ctgtacgaac ttatattcta caaatatgct
    12901 gcaactctgt tgcaaaaaca gccacagagt ggctaaatct ttttagcaag attttacaat
    12961 actgaatttt tgctacagag tagcagtaca taatctttat cggacaataa tgatttttta
    13021 aattgaaatt acggtgagtt ataactcagg ttaaccaagg tattgaaatt taatctgctt
    13081 tttcacttat ttcaaaaatt ttgttttacc ttcgtacaga gttttcctgt taacatattg
    13141 aacgaacgta aaatgaaaaa atctttaact gtttttacaa tttttattgc tattctgttt
    13201 gccgcttccg ctaaagctca actttcggtc acttcttatt ccatttatgc ccttggcatc
    13261 agcaccgata gccggaagcc tttcagtgcg gaactcaaaa cttttgccaa taccggcttt
    13321 tatgaccttg gtttcgaact ggatttgttt cataatttca aaacgcacga gtatcaccgc
    13381 ttttccgttg gattgggctt aaacagtatg ccttttaacg aagtttatct gaatgaaata
    13441 actcttccgc tggtgctcga agtttttccg cttaaaaact ttaaaaagct gtcgctgctt
    13501 gtggagtttg cccctgctat ggatgtcaat gtggatgatg ctggtgactt tagtttccgg
    13561 catttgtggg gcatcagata ccggtttaat taaaatattc aactatgaat ttgagaacag
    13621 tcaatgtaaa tcgctatatc atgccgttgc gcgaaggtgg atcgctgcct gcacttgccg
    13681 aagccgacga tggttttaac tatgttgtta agttccgggg ctcaggacac ggaactaaga
    13741 tgctgatttc cgagttgata ggaggtgcgg tagcgaaata tctcaatttg aaaattccgg
    13801 aactggtttt tgttcaactc gatgaagctt tcggtcgatc ggaaggcgat gaggaaattc
    13861 aggatttgct caaagccagt acaggtctga accttggact gcatttttta tccggagctc
    13921 tggcttacga tccggttaca acaaaaattg atgcagctct ggcctcaaag atagtatgga
    13981 tggatgcatt tctgaccaat gtggaccgca ctgtgaagaa taccaatatg ctgatgtggc
    14041 acaaggaact gtggctgata gatcatggct caacacttta tttccaccat acctggacaa
    14101 attgggaaaa gcaggcagta cgccctttcc cgcttataaa agatcatgtg ttgctcgaat
    14161 ttgcttcaca actacatgaa gctgatgaag aatgccgcga catactcaac gatgaagtac
    14221 tgagcgaagt ggtgaatctc attcccgatg aatgggcaca ctggaatgaa aacggggaaa
    14281 cacctgaaat catccgggaa atctatttca aattcctgag taaaagaagg acacaatcgg
    14341 aaatattttt aaatgaagct ctcaatgcgc ggaaaatact tttatgaata cgcggtgata
    14401 cgtcttttcc ctaaggtgga acgcgaagag tttattaata tcggaattat tttgttttca
    14461 aaagaggcaa aatatattgg catgaaatac agaccagata ttgaacagat ttgccggact
    14521 gcgaccgaac tagattttga cttgcttggc aataccctga aatctttctc aaagatttgt
    14581 aacggtgacc agtcgggtgg taaaatagct gagatggaca tagcagagcg ttttcgctgg
    14641 cttacagctg tccgcagtac ctgcatacag acttcacgac cccacaatgg atttaccgat
    14701 aatccggaaa agaagctgga cgaattattc gccgagttgg taatgtaatt ccttttttct
    14761 aaaaaaatcc atcagatatg ccgagaaccc gaagactgcc gaaatccgta ttttgtttgt
    14821 ggtttttatt catactgatc ggtcaggctg cctgtcgacc ggttgatgag cagcaaccgg
    14881 ccgaaaatct tgttgaactg gatagtatgg cttaccggta tcccaggtat gtgtataatg
    14941 tcgtcaacaa tctgattaaa cagaatcaac agaccggtta tgaccgtgcc tatacaggtc
    15001 tgatcctgtc ggtagctgct gaaaacatgg gcagatcctt gccttcggat agtatgatta
    15061 cggcaacggt caatgctttt tccatgcgtc ctgaatataa tcccggtaat tacctgaggg
    15121 ctctgatata ccagggaatt acccggtttc ataccggcat tgcggacaac agggcatatc
    15181 ttccgattaa agaagcttta attctttcag aaaccactcc gactgaccag aaaactttgc
    15241 agattgcctg ttattacctg ggcgtcattc acaccaaaaa caacaacgtg aaccaggcgc
    15301 acgaatactt caaaaaggcc tttgtcattg cagagaattt gaaagacagc gtggtgctat
    15361 tcaaaacctg ccgcgaaatg tactggaacc gcatgaaagc gctagatttc ctcactgccc
    15421 gaacgctgct cgaaaccctg caatcttttt cgaatgtttc cccggatcaa atccgtgata
    15481 tccggaatgc ggaagcaata tattacaaca gcctgaagcg ataccgaacg gcacttaaaa
    15541 tagattacaa cctgcttcgt accgatatac aaaacaagga tagcagcgcg ctgcttgccg
    15601 attattatcg catttcggac aactacaaat tcatgaacca acccgacagc gcattgcatt
    15661 acggcgaact ggcaacccgt aatatccgcg acaccacctt ctacctcaac tattactact
    15721 acctgaatgt ggcagaattg gcatcgcagt tgaagatgtg ggataaaagc accgctgcct
    15781 atcagcaggt ttaccgcttg atgaataaaa ccatcaacaa tcagctcaat acgcagataa
    15841 tggagttgga acgtaagtat gatgtcgctt cggcagagaa cagggccatt aaacttaaaa
    15901 acaacaatat agtcctccgg ttggtgctta taatcctgac catggggctg ataatcaccc
    15961 tgctgatttt cagaaaccgg tcgcggctgc agatggaaca gcaaaaatcg ttattgcagg
    16021 aaaaaaagat actcgaacag aagcagctga tagcggaagc caatgaaaag cagatgatgc
    16081 tcgacaagca gcttacggaa agaaaactga tagagaaaca atttgtgata cccatttacc
    16141 ggcagatatc gcagcgcaat aacgcgataa aaaattttct gctcgatttg caaagcaaca
    16201 gctacatcac caaaaatcct cagatactgc aaaagattga aaacgaatac cgcgactttg
    16261 tgcaaaccac ccgagttacc gaaccacaat tcctgactga cgaacttttc tccgggctta
    16321 ccggaataag tacagaagac agcggcctct tcaacgaaag tgaaaagatg atgctggcct
    16381 ttatggctac cggatccgac aatcagcaga tggctaccct gatgaatact tcggtggaaa
    16441 gtatacgggt tcgcaaatcg aaactgaaga aaaaaatgca ggagaataag gtgaaaattc
    16501 ccaaaaaact ggaggctgac atgtaatgcc ggtacgaaaa acagtctgat tatcatttcc
    16561 tttccgttaa ttaaatttgt gtaaatgcct gtaaagtaat ttgataacgc cgtttgggcg
    16621 ttaacttcag gacaggcccg tacaaaacct ctgtacgggc tttaactttg tcatacgcaa
    16681 aacaaagcag tttcagttcc aaataaaatg cagaataaac aacttcatct tacgaccgga
    16741 aaaaaagaac tgcctgttca ggtaaaatcg ggttacttaa caattaaaaa tttaatcgac
    16801 atggcacacg acattaattt aacttctcag gaatggttaa acatcatttt tgaaggaaag
    16861 aacaaagatt acggcgcata caggcttcgt caggattcat ccagacggca tctgattgct
    16921 tttatctcag tcgcagttct gacagttctg gctattacag tgcccgggat atccaaagag
    16981 ctcgggatag gaggcagaga gaggaatagg gaagtcattc agttaatcga cgctaacaat
    17041 atgacaaaac cggaagtgcc tgaagaacat ataaagaagc aatatcaggc tccgcctccg
    17101 gttgaactga aaagtaccat gaagtttaca gcaccggtaa tcaagaaaga tgagttggta
    17161 aaagaagaaa atgaggtaaa atctcaggat gaaattacag aatcaaaagt tgccatttca
    17221 gtagctgatg taaagggtac caatgaagat acaggtattg atattgctat attaaatgag
    17281 catcacgtga ttgtagctga tacggaaccc gaaaaagtat ttgaatacgt agaacagcca
    17341 ccttcgtttc cgggcggaga tgcagcgctg atggattatc ttaatggaaa tatcagttac
    17401 ccgactattg ctcaggaatt ggaaattcag ggaaaggtaa cctgttcctt tgtggttgga
    17461 aaagatggtt ctattcagga tgtaaaagtg gaacgaggtg tggatcgaag tatcgataag
    17521 gaagcggttc gggtaatcag ttccatgcca agatggatac cgggcaggca gggcggtaac
    17581 gccgtaaaag tgaagtatta tctgccggtg gttttcaggt tgcagtagaa tgctaaatgc
    17641 aatttaaact gcatttaaat aaaaagagcg gggagccttt attcgggctc cccgtttgtt
    17701 tatttatatt ctcacttcca cacctttgtc tttcaggaaa tgtttcagtt cctgtatgcc
    17761 tatttcctga tagtgaaaaa tgctggctgc aagcgcagcg tctgcttttc cggcttcgaa
    17821 aacatccaca aaatgctgca tggttcccgc tcctccgctg gcaatgaccg gtacattcac
    17881 cgattcggat acagcctggg ttatgtcgag cgcaaaacca tcctttgtgc cgtcgtggtt
    17941 catggaggta aggagaattt ccccagctcc cagtgccact gcctgtttaa cccagtccag
    18001 tgtcctgatg tctgtggaaa cccgtccgcc attcagaaac acataccatt cgccttcgat
    18061 gcacttggta tcaacggcaa gcaccacaca ctgactgcca aaattatcag ccaggtcttt
    18121 gatgagttgg ggattacgta cggctgccgt gttcaccgaa atcttatcag cgccacatga
    18181 aagcagtact gatacatcct caaccgatga aatgccgccg cccacggtaa atggaatatt
    18241 aatctgtccg gcaatgcgtg taaccagttc cgatagtgtt ttacgtttct cgttggttgc
    18301 cgtgatatcc aggaatacca gttcgtcggc acccatttcg gcgtatcgct taccgagttc
    18361 caccggatcg cccacatcgg ttatgttcag aaaattaatg cctttaacgg tttttccgtc
    18421 ctttatatcc aggcagggaa taattctttt tgctaacatt taattcgccc aaattgcctg
    18481 ctgggcttac agggacttat tttataaatt tatatactct ttccggatgt tctaactcgg
    18541 ggttaggaga ggggtgtagc attcagcgct gttttatctt cgttccagtc acccaaaata
    18601 acagtttcca ctgtattcac cagttttttg tcatagtttc tttccgcacg aatgtagttt
    18661 tcggtaaatc catgcatctt ttctccttcc tttttggctt cccagagtac ctgaactgtt
    18721 tttcccgact gtgagttgta aaaagcttcc gttttcccgg cggataaatg atgaaggctt
    18781 tcactgcgtc gtttgcgttc ctttacggga acgatatgtt ctatctcaag cattttggta
    18841 tttgcccgct cggaataggt gaatacgtgg agttgtgaaa aatccagact ttgaataaat
    18901 tcaaacgact gttcgaacaa ttcatccgtt tctcccctca ctcccacaat cacatccaca
    18961 ccgataaaag catccgggag aatgcgtttg atagtggcaa tcttttctgc aaacaattca
    19021 cgcgtatagc ggcgtttcat cagtttaagt atctcgtttg tgccactctg caacggaata
    19081 tggaaatgag gcatgatgtg gcggctgtcg gcaacaaact gaataatttc atcagtcagc
    19141 agattaggtt ccaccgatga aatccggtag cggacatccg cttcgatgtt gtccagcgcc
    19201 tggagtaatc cgatgaatgt ttcgccggtg tgtttgccga actctccggt attgacgcct
    19261 gtcagtacaa tttcctttgc acctgcacgg atagctatca tagctgtttc cactgtttca
    19321 gcgatagttc cgctccggct gtgaccgcgt gccagcggta tggtgcagta ggtgcaatag
    19381 tagtcgcatc cgtcttgaac cttcagaaag taacgggttc ggtcatcgta cgaaacggaa
    19441 ggtttgaaat ccttcacttt caggatatca gcccgtagaa tttcaccttt ggattccgga
    19501 tgattgttgt tcaggtaatt ctgtatcgaa aatttttcgt tggctcccag taccagatcc
    19561 actccttcta tcgtggcgat ttcttccggt ttaagctgtg catagcaacc cgtaactatc
    19621 attttagcgt tgggatgctg gcggtgcagt ctgctgatag cctgacggca tttgtggtcg
    19681 gctgtatcgg ttaccgaaca ggtatttatg atgcagatat cggcttgttc gccgtggcgt
    19741 actttcataa atccttcttc ctccagttgc ttcccgatag ccgatgtttc ggcaaagttc
    19801 agtttgcaac ccagcgtatg aaatacgacc tttttattgt tgaatatcgt gttgtcaatc
    19861 atttctttat aatggaaatt gtatttgtcc gtgagaaatc ttc
//
LOCUS       DAOPYB010000037        19606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2887_length_19606_cov_8.953404, whole genome shotgun sequence.
ACCESSION   DAOPYB010000037 DAOPYB010000000
VERSION     DAOPYB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19606
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_19606_cov_8.953404"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKH58_04625"
     CDS             <1..182
                     /locus_tag="PKH58_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNX88343.1"
                     /translation="QPGCSACLAMNDDKVPAGKYAVSTSNRNFEGRQGPGARTILAGP
                     LVAAAAAVNGVITAP"
     gene            247..900
                     /gene="leuD"
                     /locus_tag="PKH58_04630"
     CDS             247..900
                     /gene="leuD"
                     /locus_tag="PKH58_04630"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNX88344.1"
                     /translation="MQTSNNTNKEKNAQTPPSGGWGAGSLTSTCVPLPIENVDTDQII
                     PARFLKATDKNGFGDNLFADWRYNKDGSPKPDFVLNNPKYSGEILVAGKNFGSGSSRE
                     HAAWAIAGYGFKVIVSSFFADIFRNNALNNGILPVVVSPGFLAEILEAVVANPANTLT
                     VNLEEQKITNNATGKSEIFEINSYKKECLLKGLDDIDYLLSKRELIEEWEKRQTPDR"
     gene            1034..2566
                     /locus_tag="PKH58_04635"
     CDS             1034..2566
                     /locus_tag="PKH58_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNX88345.1"
                     /translation="MDTTLRDGEQTSGVSFAAQEKVSIARLLLEELGVDRIEVASARV
                     SEGEFQSVKKIAQWAQAHGHLDQVEVLGFVDGTASLNWISNAGCRVVNLLCKGSLKHC
                     KYQLRKTVEEHLADIKAVIADAVNMGIKVNIYLEDWSNGMRESEDYVFQMVDELQHQP
                     IMRFMLPDTLGVLNPDETRDFCQRMISRYPNLHFDFHAHNDYDMAVANVFEAVKAGVK
                     GVHVTVNGLGERAGNAPLTSVIALIHDHLKLQTNVKETSVFHVSKVVESYSGIRIPNN
                     KPVIGDNVFTQVAGIHADGDKKNNLYFNDLMPERFGRTRAYALGKTSGKANILKNLEL
                     LGIELDDESMKKVTERIIELGDKKELVSTDELPYIISDILQNDIEKQDIRMLNYSLTL
                     TDGLRPTANVKISIRDEVYEESAAGDGQYHAFSKAMYKIYKRLNKPTPELVDYVVVIP
                     PGGQTDAFVQTIITWKFEGKTFKTRGLDIDQTVAAIKATMKMLNLIESGNIPTINYME
                     LP"
     gene            2766..3839
                     /gene="leuB"
                     /locus_tag="PKH58_04640"
     CDS             2766..3839
                     /gene="leuB"
                     /locus_tag="PKH58_04640"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNX88346.1"
                     /translation="MKTLKIAVLPGDGIGPEIIKQALDVTQTVCDKFGHKLEYDYGIV
                     GACAIDETGDPYPAATHELCMQSDAVLFGAIGSPKYDNDPNAKVRPEQGLLRMRKTLG
                     LYANIRPVTTFPSLIHKSPLRADLVKGADFMCIRELTGGMYFGRPQGRSEDGNTAYDT
                     CVYTREEIERILHLAYKYADQRRKKVTVVDKANVIATSRLWRQIAQEIAPQYPDIETE
                     YMFVDNAAMRIIQWPKSFDVLVTENLFGDILTDEASVITGSLGMLPSASIGLHTSVFE
                     PIHGSYPQAAGKNIANPLATILSAALMFEYAFGMMNEGAIIREAVTASMDAGVVTEDI
                     SEGGKAYSTSEVGEWIVKWIKEK"
     gene            4037..5641
                     /locus_tag="PKH58_04645"
     CDS             4037..5641
                     /locus_tag="PKH58_04645"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNX88347.1"
                     /translation="MNKNFLFIIAILLSFHRTFAQEVKITNPTVIQDNNYSARGNNNE
                     EILIIKLATSGINPVEFSSLKMNMNGTTNVKDVAQILIYQKTTKGQFDPRNPLKSSVL
                     LGTTKPSKRDIVIPLKGKLASDTTCIYVTYDVREKAGEGNKLDASVISLTAGSYTHFF
                     KAGNPEGAREILLRRTLVYTPNDYGSKNYRIPAIITAKDGSLVILTDKRKFNGTDLPE
                     DIDVVANRSTDGGKKWSEPVTVAEGKGRGKGFGDALIMKAKSGKLVALFVGGTGLWGS
                     TANNPQRTYVSTSTDNGITWSAPRDITNQIYGYENSDPVRSKWISLFFGSGHGLCTRS
                     GRLMGVLVVREPGKNGLHNYAVYSDDEGENWKVSETAIVNGDEAKVIELNNGDILMSS
                     RQPESYGNRLWAVSSDGGITWGTRNTWSEIWGARCDADIVRLTSTLDGFDKDRILHTM
                     PNDKSRRNVTMWMSYDEGKTWPLKKTIAPNTSGYSSITILPDGTIGVYLEEDEKSNYK
                     MYFINFSLNWLTNGKDKYIPQKRKKR"
     gene            complement(5726..6982)
                     /locus_tag="PKH58_04650"
     CDS             complement(5726..6982)
                     /locus_tag="PKH58_04650"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNX88348.1"
                     /translation="MIVYKFGGASVKSSDGIKNISKIVSLVKEDLFIVVSAMGKTTNA
                     MEEVLDFFMKADKESALKKMEEVEKYHYEIIGELFENKTIGENAVKPLFDELKDLITT
                     GVGTDYDRWYDRLVSYGEVISTKIVSEFLLTQGVHNSWLDMRQLLVTDSNFREANVHM
                     IESEKRLRAAVDFKQSKVFIGQGFIGTNMQGAPTTLGREGSDYTAAVVGNLLNAESVS
                     IWKDVPGILNADPRIFPDTVLIPELTYMDAVELAYSGAQIIHPKTIKPLENKSIPLYV
                     RPFGNPSEAGSVIKSSTSKPIDIPVLILKKNQMLVTLRPLDFSFILEESLTWIFTVVE
                     KYRLKVGLIQSSAISISICVDNNRYLQRALDEFANDYKVSYNTDLELLTIRGTNPEII
                     EQTTHGREILVQQNTRRIARILMREK"
     gene            complement(7080..8324)
                     /locus_tag="PKH58_04655"
     CDS             complement(7080..8324)
                     /locus_tag="PKH58_04655"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNX88349.1"
                     /translation="MKKLCVLILLVICLPLAGQPVNRTQILHDNIKTLQVKVDDNPVC
                     LPVIALNGSETLQISFDEMSHNIHSYNYDVKHCNADWSLSALSTTEYIDGFTTGKIDD
                     YSLSVNTTFLYTHYRIALPNNEMKLKISGNYVMNIYEDNQQDDPIAKVCFSVVESRVQ
                     IDAAVRGNTDTELSGRFQQLDFDLLFNDYQIRDAQSEIKVVVRQNNRIDNQVTDLKPS
                     YFSAGKLTYKNNRNLIFEGGNEYHRFDISSIYSFDEGVNNIDFVRPSYHAYLRQDEII
                     ARNPYITNPDVNGKFIVNLQNASDDDTEADYMYVHFYLLANEPFLNGNIYVGGEWNYN
                     QIDKTSLMRYNSENRMYYNNVLLKQGGYNYQYWMIPQGETKAQVSPVDGSHWQTLNEY
                     AIYVYHRPWGGRYDKLVGVKIL"
     gene            8952..10280
                     /locus_tag="PKH58_04660"
     CDS             8952..10280
                     /locus_tag="PKH58_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HNX88350.1"
                     /translation="MNLPEPFVRRTQELLGDEFPDFLDALDIPSPVSVRVNDKTAYVP
                     SDDRVKWCDSGYYLSGRPVFTLDPLFHAGVYYVQEASSMFLQQAMSQFVAPAGVVLDL
                     CAAPGGKSTLISQFLKTGGMLVSNEIVRSRAFILVENLIKWGNDAVVVTNNVPADFQR
                     LPSFFDAVVVDAPCSGEGMFRKDAGAIDEWSEQNVEMCARRQRDILTDVWDTLKKDGI
                     LIYSTCTYNREENEKNVSWIETELGAEILRIDTAAYPQITVSDKGYRFFPHKTRGEGF
                     FLSVLRKTSDAPSLRMKKQEKRKLKPEFDETEFKNRLMEPEDWKIYSDNNFISAFRLK
                     FSTEVELLKNNLHIMHYGITLCEIKGRDLIPHTSLALSKSINTEKIQQAEVELRDALL
                     YLKKEAINLPDAEKGFILVKYQNIPLGWVKNIGNRTNNLYPNEWRIRMKI"
     gene            10337..11122
                     /locus_tag="PKH58_04665"
     CDS             10337..11122
                     /locus_tag="PKH58_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX88351.1"
                     /translation="MKSSLSTPLRGRGATITFLGTGTSTGVPQLGCKCPVCRSASPKD
                     KRLRNSALITVDDKRFLIDAGPDLRQQLITNDIFHVTAILLTHEHYDHVGGLDDVRPL
                     GETNIYAEANVLEAIERNMPYCFGVIKYPGVPKMNLHKVDMNPFQIQGVNIIPIRAMH
                     ARLPVLGFRIGKLAYLTDVKSIDNTELDKLRGLKVLVINALRKEEHISHLSLSEAISI
                     AQQVNAENTYFTHISHHLGLHEDVSAELPENIHLAYDNLSINF"
     gene            11170..12255
                     /locus_tag="PKH58_04670"
     CDS             11170..12255
                     /locus_tag="PKH58_04670"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HNX88352.1"
                     /translation="MQARNRSALMRLTVSKILQTVGLIFLMSSCTTSRMFTTLDILRP
                     AQVTFPPEINSILIVDNSVAQPSNVGHYNKKLFEEYTSVEQVFDSASIFCTASLRESL
                     EEKGFFKNVHMAKNRTNLTEKPYKITPLTSEAVKSLCAVYQADAILSLDRILHIDKIT
                     ENIYPDDLHFFSNLDIQMGTVWSINYPTDKPTTYLQFADSFTWESSNTIRSKAGDKLP
                     SRYNALVDASLLTGTNIADRMIPRWEKEDRYFYNPRNKYMRQAMDSVEVRNWKAAIDL
                     WKLAAEKSGYATKYRANYNIAIASEILGDMENAILYCDLAIEIYPKVLFAFNIDAEND
                     IYKLMSYKEELQKRAKEINLIKKQLGN"
     gene            12292..13809
                     /locus_tag="PKH58_04675"
     CDS             12292..13809
                     /locus_tag="PKH58_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNX88353.1"
                     /translation="MIQPNDEKLLQKKGISAQQIEEQLKSFKSGFPYLIIRSSASVGN
                     GIIRVNETELQTYLNEWGKYLTTNARVLKFVPASGAASRMFKDLFSFLEGDSDLPVTG
                     FEKKFFDDIEKFAFYDDLNAVCKKNTGKNIPELKKANSYREIVANLLLEKGLNYGALP
                     KGLLKFHSYKDDKRTPLLEHLVEGAVYASTISGNVNIHFTVSKEHRQLFEKHLGENLS
                     KYEKRFGVKYNVSFSEQKPSTDTIAADADNNPFREEGELVFRPGGHGALIENLNDLDA
                     DIIFIKNIDNVVPDALKDTTITYKKLIAGVLVHFQQQAFAYLKELENEKISDAKLTII
                     AQFCEKKLNNHNENIKSLSGGCLREYLIGKLNRPIRVCGMVKNVGEPGGGPFLTVNSD
                     GTVSPQILESSQIDMNNTADKEKMQNSTHFNPVDLVCGVKDYKGRKFDLLKYVDKNTG
                     FISSKSKNGKELKALELPGLWNGAMSDWNSIFVEVPIETFNPVKTVNDLLRKEHQ"
     gene            14290..14850
                     /locus_tag="PKH58_04680"
     CDS             14290..14850
                     /locus_tag="PKH58_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNX88354.1"
                     /translation="MASDEKEEIEMLKNASAESFETLYFRYSARLYNFVLKLSKGNNF
                     MAEELVQRTFIKVWEKRDQINPEQSFISYLCTIAKNMLINEYEHQTLEFIYHKYFIEQ
                     ISLLEHHITELDVDKKLLDEYIEKLTAQLPPKRREIFILSKRNGLSNKLISEKLKISE
                     STIETQLSKALKYMKEQLRLLNECVS"
     gene            14948..15985
                     /locus_tag="PKH58_04685"
     CDS             14948..15985
                     /locus_tag="PKH58_04685"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HNX88355.1"
                     /translation="MKKEEILQLFERYILNTASPDEVKKLCHWVNNNLEISNWLENQI
                     LHSSDEIDKELQLRMFKDIKSSLQIEKPIEMPTVELKELTRPSIQRFKTWMLVASFLI
                     FPLISGLGVYLFMSNKKVSDPSIVSVERGQKASITLPDGSKVWLNSLSKLTYSSDFNL
                     DKRELLLDGEAYFEVAKNPKKPFVVKSNEITVEALGTAFGIKAYSEDVRVSSILMHGK
                     VRVTTPNGINILKPNERIQYDKIKRTTQQSTVTNAIDFTGWIHNELRFENESLDEIAK
                     NVQRIYNVEIIFASEGLKKLRYTGTVDNNSLESVLNIITLTSPISYQMDKQKVILYEN
                     KSMMKHYNRHQ"
     gene            16147..19524
                     /locus_tag="PKH58_04690"
     CDS             16147..19524
                     /locus_tag="PKH58_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX88356.1"
                     /translation="MERKNYTHLLREKYLKLCLFIFLSLFSLTLTAQITINVQNKPIK
                     EVLKVIESKSNYRVFYNEELNGLDKLVSLQVNNAGIEETMRLILLNSEISYKLEGNNL
                     IVLMAKVKENQGLSKKITGVVTDINGDYILGASVLIKGSKTGALTNMKGQFSLDVSDN
                     SLLIISYIGYKTQEISVAGKNSIQVKLIEDVELLNEVVVTALGIKREEKALGYAVQAV
                     NGEKLQTVKGVDMATSLTGKVAGLMVKNSTEFAQSPDIQIRGENPLIVIDGVPYGNMT
                     LKDIPSDDIENLSVLKGATASALYGYRGAGGAIMVTTKKGNKNKGLEVSLNSGSMFTA
                     GFLAIPELQSTFGRVVNTATNTYSTGGDGSWGIPMDGREVKQWDPVTKTWLLMPYLPR
                     GKNNFKNFLEQGYVLNNNVNIVQQGEFGSFRASATWVNNKGQYPNSDYNKYTYGIGGD
                     MKLNKFLLSSSISYNKQTSPNIGFSGYTSYDPMYSMLVWSAADYDVRDYMDYWLIPNE
                     SQNNSYTDSSNNPYFDRFQRTHSINKDIMNGNLSVNYDFTPWLKLSLRSGFDTYSDRQ
                     DIKISKGSLISAGSATLIPNGTQVWGESANGSYNTGISRGYSFNNDLILSGSKTFDKF
                     SVDGLFGGTIFYKQDEGIEAKTQDGLTIPGYYSLNASVSPANVSSTIYRRQVNSLYGR
                     LAMSWNNLIYAEGTMRNDWSSTLSQSTRSYLYPSVSGSFIASELLPKTDWLSLWKIRG
                     SWTTSKTPADVYAINSVYTITNSAWGSFSTASYPGSIRSSVVMPESSETFEVGTAINL
                     LKNRASLDFSYYSKRMYDGLTWGGISSASGFTSNYVNTDEEVTRRGIEITANVTPVKT
                     KDWQWDLSVNWSKYARYFTKLDSLYSSDKPWVKVGERYDAYVLYDYLRDPDGNIIHNA
                     GKPLYSDYTSKFGYSDPDWIWGLSSSVRYKNFQFSVSMDGRVGGLTQTITEMYMWRAG
                     SHPNSVTDQRYLDATVGGNNYIGEGVKVVSGSATYDTYGNITSDTRVYAPNDVATTYE
                     TYINALHKGTAWGGAASPIDVYSTTFLKIREMSLTYTLPKHICEKIYTKGISLSTVGQ
                     NLFLWSKQFKYSDPDGGYDNFSDPSIRYIGFNVKVSF"
BASE COUNT         6252 a         3716 c         4050 g         5588 t
ORIGIN      
        1 gtcagcccgg ttgttcagct tgtttggcca tgaacgacga taaagttccg gcaggcaaat
       61 atgctgtttc cacttcgaac cgtaactttg aaggacgtca gggacccggt gcacgtacta
      121 ttctggctgg tccgctggtt gctgctgcgg ctgcagttaa cggagtaatt acggccccct
      181 aaccccctga agggggaatt tggaacgaaa aatcaaattt tatagaaatt actaactttg
      241 aaaaaaatgc aaacttcaaa taatacaaat aaagagaaaa atgctcaaac tcccccttca
      301 gggggttggg gggcaggtag cttaacatca acctgtgttc cgcttccaat tgagaatgtg
      361 gatactgacc agataattcc ggcccgcttc ctgaaagcga cagataaaaa cggtttcggc
      421 gataatctgt ttgccgactg gcgatacaat aaggacggaa gtccgaagcc ggattttgta
      481 ctcaataatc ctaaatattc cggtgaaatc cttgttgcag gaaaaaactt cggttcgggt
      541 tccagccgcg aacatgctgc ctgggcaata gcaggatatg gtttcaaagt tattgtatca
      601 agcttttttg cagatatatt ccgcaacaat gcgctgaata acggaattct accggttgta
      661 gtttcacccg gttttctggc tgaaattctt gaagcagtgg ttgcaaatcc tgctaataca
      721 ctgacggtaa atctcgaaga gcagaaaatc accaacaatg ccaccggcaa atccgaaata
      781 tttgaaatca attcatacaa aaaagaatgt ctcttaaaag gccttgatga tattgattat
      841 ctgctaagta agcgggaatt aattgaggaa tgggaaaaaa gacaaactcc tgaccgctaa
      901 ggagaaattc gagagtattc tcatcatttt cggtactaaa aaaaagaata tgaataatac
      961 tgctaataaa gataacgctg aaaaacatcc tcaaactccc ccatttgggg gccggggggc
     1021 aaaaatcgaa ataatggata ccacgctgcg ggacggcgag cagacatcag gagtttcatt
     1081 tgcagctcag gaaaaagtca gtattgcccg cctgttgctc gaagaactgg gcgttgaccg
     1141 tattgaagta gcgtcggcac gcgtgtcgga gggtgaattt cagtcggtga aaaagattgc
     1201 acaatgggca caggctcacg gacatctcga tcaggttgaa gtgcttggat ttgtggatgg
     1261 aactgcttcg ctcaactgga tatcgaatgc tgggtgccgg gttgtaaacc tgctttgtaa
     1321 aggttcactg aaacactgta aatatcagct tcgtaaaacg gtggaagaac atctggctga
     1381 tatcaaagca gtgatcgccg atgccgtaaa tatggggatt aaagtcaata tctatctcga
     1441 agactggtcg aatggcatgc gcgaatcgga agactatgtt tttcagatgg tagatgaact
     1501 gcaacatcag ccgattatgc gttttatgct gcccgataca ctgggtgtgc ttaatcccga
     1561 cgaaacccgc gatttttgcc agcgaatgat cagccggtac cccaaccttc actttgactt
     1621 tcacgctcac aacgattatg acatggcagt ggccaatgtg tttgaagctg tcaaagccgg
     1681 agtgaaagga gttcacgtta ctgtgaacgg attgggtgaa cgtgccggaa atgcaccgct
     1741 taccagtgta attgctctga tacacgatca tctgaaactt caaaccaatg tgaaagaaac
     1801 cagtgttttt catgtgagta aagttgtcga aagttattcg ggtattcgaa ttcccaacaa
     1861 taaaccggtt atcggagaca atgtatttac acaggtagca ggtatccatg ctgacggtga
     1921 taagaaaaac aacctgtatt tcaatgatct gatgcctgag cggtttggac gaaccagagc
     1981 atacgcgttg ggaaaaacat ccggaaaagc gaatatcctc aaaaatctgg aactgctggg
     2041 catcgaactc gatgacgaaa gcatgaaaaa agtgaccgaa cggatcatcg aactgggtga
     2101 caaaaaagaa ctggtgagta ccgatgaact gccttatata atttcggata tactgcaaaa
     2161 cgatatagaa aaacaggaca ttcgaatgct caactattca cttacgctta ctgacggatt
     2221 gagacccaca gccaatgtga aaatatcaat acgggacgag gtttacgaag aatccgctgc
     2281 cggtgacgga caatatcatg ctttctccaa ggcaatgtac aagatttata aacgtctgaa
     2341 caaacccact cctgaacttg tcgactatgt agtggtaata cctccgggag gacaaaccga
     2401 cgcgtttgta caaacaatta ttacatggaa atttgaaggt aaaacattta agacaagggg
     2461 attggacatt gaccagaccg ttgcagccat caaagcaacc atgaaaatgc ttaacctcat
     2521 cgagagtggt aatatcccga caataaatta tatggaattg ccataatgga gctgccccct
     2581 aaccccctaa aggggggata gcagcaggga caatcagctc tgatgattac tagaaaattt
     2641 gacaatactc aattcctttg aaacagataa ctcaagatta tttaacttta cagaaatatt
     2701 aaaataaact taatcaacgc tttcttagtt cccccttcag ggggttaggg ggcagatatt
     2761 tatttatgaa gacattaaaa atagcagttc ttccgggcga tggtatcgga ccggaaatca
     2821 tcaaacaagc gttggatgta actcaaaccg tatgtgataa atttggccat aaactggaat
     2881 acgactatgg catagtcgga gcctgtgcca ttgacgaaac cggagatcct tatccggctg
     2941 caacccacga actctgcatg cagtccgatg cggtactatt cggggcaata ggttcgccca
     3001 agtatgacaa cgacccgaat gcaaaggttc gtcccgaaca gggattgctg cgcatgcgta
     3061 aaacactcgg gttgtatgca aatatccgtc cggttacaac gttcccgtca ttaattcata
     3121 aatcaccact gcgtgccgat ttggtgaaag gtgctgactt tatgtgtatc cgggagctga
     3181 ccgggggcat gtacttcggt cgtccgcagg gacgcagcga ggatggaaac acagcttacg
     3241 acacttgtgt atatacccgt gaggaaattg aacgtattct acatcttgct tacaaatacg
     3301 ccgatcagcg tcgtaaaaaa gtgaccgttg tggataaagc aaatgttatt gccacatcac
     3361 gtctctggag acagattgcc caggaaatag ccccacagta tcccgatata gaaaccgaat
     3421 atatgtttgt ggacaatgcc gctatgcgca tcatccagtg gcctaaaagt ttcgatgtgc
     3481 ttgttaccga aaatcttttc ggagatatcc tcactgacga agcttccgtt atcacgggtt
     3541 cgctgggaat gcttccgtca gcttcgatcg gattacacac ttcggttttt gaaccgattc
     3601 atggctccta tccgcaagct gccggtaaaa acatagccaa tccactggcc accattcttt
     3661 cggccgccct gatgtttgag tatgcatttg gaatgatgaa tgaaggggca attatccggg
     3721 aagccgtaac agcaagtatg gatgccggtg tggttaccga agatatttcc gaaggaggta
     3781 aagcatattc taccagtgag gtgggagagt ggattgtaaa atggataaag gagaaatagt
     3841 gttttgttaa gaaaacagtt tcttcaatta gcctgagaaa aatcagactt atttataaag
     3901 gatgaaatga acctgttact atctgtatga taagttagct ataaccattt tttgcctgga
     3961 ataaatcagc tgattttgtt acttttgcaa tagtttataa tacaattgat taaaatcatc
     4021 acaaccttta atttctatga acaaaaattt tttattcatc attgcaatct tactaagctt
     4081 tcataggaca tttgctcagg aagtgaaaat tacaaacccg acagtaattc aggataataa
     4141 ttacagtgcc cggggcaaca ataacgaaga aatcctgatt ataaaactcg ctacttcagg
     4201 tattaatccc gttgaattct cgtcgttgaa gatgaatatg aacggaacga cgaatgtcaa
     4261 agatgtagcg caaatactca tttatcaaaa aaccacaaaa gggcagttcg atccgagaaa
     4321 tccgcttaaa tcgtccgttc tgcttggaac cacaaaacct tcgaagcgtg atatcgtgat
     4381 tcctctcaaa ggaaaattag catcagatac cacctgtata tatgttacct atgatgtaag
     4441 agaaaaagcc ggagagggta acaagcttga tgcgtctgtt atttcgctga ccgcaggaag
     4501 ttatactcac ttttttaaag cgggaaatcc cgaaggcgct agagaaatat tgctgagaag
     4561 aaccctggtt tacactccaa acgattacgg ctcgaagaac tatcgcattc cagccattat
     4621 cacagctaaa gacggctcgc tggtaatcct gacagataaa cgaaaattca atggtactga
     4681 tttacccgaa gatattgatg tagttgctaa cagaagcact gacggaggta aaaaatggtc
     4741 cgaaccagtc actgtggcag aaggaaaagg aagaggcaaa ggatttggtg atgctttaat
     4801 tatgaaagca aaatcgggta aactggttgc tctttttgtg ggtggaaccg gtttatgggg
     4861 atctactgca aacaatccgc aaagaacata tgtttctaca agtaccgata atggcataac
     4921 ctggtccgct ccacgtgaca tcaccaatca gatttacggc tacgaaaaca gtgatcccgt
     4981 tcgctcaaaa tggattagtt tatttttcgg ttccgggcat ggtttgtgta ccagatccgg
     5041 tcggttaatg ggagtcttgg tggttcgtga acccggaaaa aacgggctgc acaattatgc
     5101 cgtttattca gacgatgaag gtgaaaactg gaaagtatct gaaactgcca ttgtgaatgg
     5161 tgatgaagct aaagtgattg aactgaacaa cggtgacatt ttgatgagca gccgtcaacc
     5221 cgaatcatac ggtaacagac tttgggctgt ttcatcggac ggaggcatta cctggggcac
     5281 aagaaacacc tggtctgaaa tctggggtgc aagatgcgac gcggatattg tcagactgac
     5341 ttccacgctg gatggttttg ataaagaccg tatattacac acgatgccga atgacaaatc
     5401 acgcagaaat gttaccatgt ggatgagtta cgacgaagga aaaacctggc cgctgaaaaa
     5461 aacgatagcg ccaaatactt ccggctattc atcgatcact attctgcccg acggaactat
     5521 cggtgtttat ctcgaagagg atgaaaagag caactacaaa atgtatttta tcaacttttc
     5581 actcaactgg cttacaaacg gaaaagataa atacatcccg caaaaaagaa aaaaacgttg
     5641 atattttcaa cagtaaatac ataattagct gccagttgaa tgattggcag ctaatttttt
     5701 atattcaaac aagatttatt gatatttatt tttcccgcat cagtatccgt gcaatgcgtc
     5761 ttgtgttttg ctgaacaagt atttcccttc catgggttgt ttgctctatg atttctggat
     5821 tagttccgcg aatggtgagc aattccagat ccgtattata acttaccttg taatcatttg
     5881 caaattcgtc cagagcgcgt tgcaggtagc ggttgttatc cacgcagatt gaaatactga
     5941 tagccgagct ttgtatcagt cccactttca accggtattt ttctacaact gtaaaaatcc
     6001 acgtgaggct ttcttccagg ataaaagaaa agtcgagcgg acgcaaagtt accagcatct
     6061 gatttttttt gagaataaga accggtatat caattggttt cgatgtggat gacttgatca
     6121 cagagcctgc ttccgaggga tttccgaacg gacgaacata aagtggaatg gatttgtttt
     6181 cgagtggttt tatggttttc gggtggataa tctgagctcc actgtatgcc agctctactg
     6241 catccatata cgtcagttcc gggatgagaa cagtatccgg gaaaattcgc ggatctgcat
     6301 tcagaatacc cggtacatct ttccagatag atacactctc agcattcagc agattgccga
     6361 caacggctgc cgtatagtcc gaaccttcgc ggccgagtgt ggttggagca ccctgcatat
     6421 tggtcccgat aaatccctga ccgataaaga cttttgactg tttgaagtca acagcggccc
     6481 gcaatcgttt ttccgattca atcatatgca cattggcttc gcggaaattg ctgtcggtaa
     6541 ccagtagttg acgcatatca agccagctgt tatgtacacc ctgtgtcagc aggaattccg
     6601 atactatttt ggtggaaatg acttccccgt acgaaacaag ccgatcgtac cagcggtcgt
     6661 aatcggtgcc gacgccggtt gtaatcagat ctttcagctc atcgaacagg ggtttgacgg
     6721 cattttcgcc aattgtttta ttttcaaaaa gttcaccaat gatttcataa tgatatttct
     6781 caacctcttc catttttttc aatgcgcttt ccttatcagc tttcatgaaa aaatccagca
     6841 cctcttccat cgcattggtt gtttttccca tggcagaaac cacaataaac aggtcttctt
     6901 ttaccaatga tactattttt gagatatttt taattccgtc cgacgatttt actgacgctc
     6961 cgccaaattt ataaacgatc atttttatgt agttgttttt gaattatgag ttgtgaatta
     7021 tgaattatga attatgagtg tgtaacctta atcagacttt aataacgcga aacctgaaac
     7081 taaagtatct tcacacccac cagtttgtcg tatcggcctc cccacgggcg gtggtaaacg
     7141 taaatagcgt attcgtttag tgtttgccag tgactgccat caaccgggct tacctgcgct
     7201 tttgtttctc cctgtggtat catccagtat tggtaattat aacctccctg cttgaggagt
     7261 acgttgttgt aatacattcg attctcgctg ttgtagcgca tcaatgacgt tttatcaatc
     7321 tgattgtaat tccactcacc acccacatag atgtttccgt tcagaaaagg ttcatttgcc
     7381 agcaaataga aatggacata catataatca gcttccgtgt catcatcgct ggcattttgc
     7441 agattgacaa tgaatttccc attaacatcc ggattagtga tatatggatt tcgggcgata
     7501 atttcatctt gccgtagata ggcgtgataa gacggtcgga cgaaatcgat gttattcaca
     7561 ccttcgtcga agctatagat ggatgaaata tcaaaacggt gatattcgtt tcctccctca
     7621 aagatgaggt tgcggttgtt tttataggtc agttttccgg cagaaaagta gctaggtttc
     7681 aggtcggtca cctggttgtc aatgcgatta ttttgccgaa ccactacctt tatttccgac
     7741 tgcgcgtccc ttatctgata atcattgaac agtagatcaa aatcgagttg ctgaaatcgc
     7801 ccgctcagtt cagtgtcggt attgcccctc acggcagcat cgatttgcac ccgggattcc
     7861 acaaccgaaa aacatacttt ggcaatggga tcatcctgct gattatcttc gtatatattc
     7921 atcacataat ttcccgaaat ttttaatttc atttcattat taggcaatgc aatccggtaa
     7981 tgagtataca aaaatgtggt gttgacagac agagaatagt cgtcaatttt tccggttgta
     8041 aatccatcga tatattcagt cgttgaaaga gcggagagcg accagtcagc gttacaatgc
     8101 tttacgtcgt agttatagga gtgtatgttg tgcgacattt catcaaaact aatctgcagc
     8161 gtttcactgc cgttaagggc aataacagga aggcataccg gattgtcatc aaccttcact
     8221 tgcagggttt taatgttgtc gtgaagtatt tgtgtgcggt ttacaggttg acctgcaagg
     8281 ggcaaacaaa ttacaagtag tattaacaca cacagttttt tcattaccaa tatacttttt
     8341 tgaattgtat tttatcgttt tgaattatca gacataatca gttccagagt tcctcctccc
     8401 ttcacttcag catgttaaag caagaatttg tcagttcttt ccaattcatc tgagctgaag
     8461 aaaagaattc cccatgcaat ggcatgtaac cgagtacgcg atttcatttt cttatagatt
     8521 ttaattgttc agatgaaacc agtatataaa attagcttgt ttgtcaaaat ttatttgtgg
     8581 catccgtttc atatgttgct gaatttaagt aaaatacctt ttgattgaat ttgaatgcaa
     8641 tattaataaa tttaattgaa taacagaagc caggatgtgc aatagaaaaa gaaaataata
     8701 caatattaag tatttattgt aacctttaaa aacatgttaa actggataat ttattcataa
     8761 aacttttatg gttttgaagt tatcatttgt aactattgat catcaacaga cattacaact
     8821 acagtcaggg aaaattccgg acaatttcag atattacaaa tacagtctgt gaaagttatg
     8881 tataacaaca gacattcgtt taataatttt ttcttccaaa ttcagtatat ttgcacccta
     8941 aaaaggattt aatgaattta cccgaaccat ttgtcagacg tactcaggaa ttacttggcg
     9001 atgaatttcc cgattttctc gacgcgctcg atatcccttc accggtaagt gtacgtgtga
     9061 atgataaaac cgcatacgtg ccttcggatg atagggtgaa atggtgtgat tcgggatatt
     9121 atctttccgg gcgaccggtt tttacccttg atccgctgtt tcatgccgga gtttattatg
     9181 tacaggaagc ctcttccatg tttttgcagc aggctatgtc acagtttgtg gcacctgcgg
     9241 gcgttgtact ggatctttgt gctgctcccg gcggaaaatc taccctgata tcgcaatttc
     9301 tgaaaaccgg cggaatgctt gtcagtaacg aaattgtccg tagccgcgcg tttatccttg
     9361 tcgaaaatct cataaaatgg ggcaatgatg cggtggtggt taccaataat gtacccgccg
     9421 attttcagcg gctgccttcc ttctttgacg ctgtggtggt tgacgcgccc tgttcgggcg
     9481 agggcatgtt tcgcaaggac gcgggagcca ttgatgaatg gagcgaacaa aatgtggaga
     9541 tgtgtgcccg aaggcagcga gatattctca cggatgtgtg ggatacactg aaaaaggatg
     9601 gaatactgat ttacagcacc tgtacgtaca accgggagga aaacgaaaag aatgttagct
     9661 ggatagaaac cgagcttggc gctgaaatcc tgcggattga tacagcagct tatcctcaga
     9721 ttacagtatc ggacaaggga taccggtttt tcccccacaa aacgcgggga gaaggttttt
     9781 ttctctccgt attgcggaaa acatccgacg cgccatcgct caggatgaaa aagcaggaga
     9841 aaagaaagct aaagccggaa ttcgatgaaa cagaatttaa aaaccggtta atggaaccgg
     9901 aggactggaa aatatattcg gacaataact ttatttctgc tttcagactg aaattcagca
     9961 cggaagttga actgctcaaa aataatttgc acatcatgca ttacggcatt actctttgtg
    10021 aaataaaagg ccgtgatctg atacctcaca ccagtctggc attatcaaaa agtattaata
    10081 cggaaaaaat tcaacaggcg gaagtcgaat taagggatgc tttattatat ctgaaaaaag
    10141 aagccataaa tttacccgat gccgaaaaag gatttatact ggtgaaatac cagaacattc
    10201 ccctgggatg ggtaaaaaat atcggtaaca gaaccaataa cctctacccg aatgaatggc
    10261 ggataaggat gaagatatga aaagcaacca atgtattgtt ttcagaaaat aaaaaaatat
    10321 gaattttata aatgatatga aaagctcttt atctacccct ttaaggggga gaggggctac
    10381 tatcactttt ttaggcacag gcacatctac cggtgttccc caacttggct gcaaatgtcc
    10441 ggtatgccgt tcggcaagtc cgaaggacaa acggcttcgg aattcagcgt taattactgt
    10501 cgacgacaag cgctttctca ttgatgccgg tcccgatttg cgtcagcagc tgataaccaa
    10561 cgatattttt catgtcacag ccatacttct tacacacgaa cattacgatc atgttggcgg
    10621 gctcgacgat gtgcgtcccc ttggcgaaac aaacatttat gcagaagcaa acgtgctcga
    10681 agccattgaa cgaaatatgc cgtattgttt cggagtaatt aaatatccgg gtgtgccaaa
    10741 aatgaatctg cataaagtgg atatgaaccc gtttcaaata cagggagtga atatcattcc
    10801 catccgggcc atgcatgcca ggctccctgt gcttggtttc agaatcggga agctggctta
    10861 ccttaccgat gtgaaaagta tcgacaacac cgagctggat aaacttcgcg gactgaaagt
    10921 gctggtgatt aatgcccttc gcaaagaaga gcatatatcg cacctttcct tgtccgaagc
    10981 catctcgatt gctcaacaag taaatgcaga aaacacctat ttcacccaca taagtcatca
    11041 tttgggactg cacgaagatg tcagtgctga attgcctgaa aatattcatc tcgcttacga
    11101 taatttatca ataaattttt aactttgcct gtcaatagct gattcctcaa cctgattttt
    11161 atgataaaca tgcaagcaag aaatagaagt gccctgatga ggttaactgt gtcgaaaata
    11221 ctccaaacag tgggattgat ttttctgatg agttcatgca ccacttcaag aatgtttaca
    11281 actctcgata tacttcggcc ggctcaggtt acatttcctc ccgaaatcaa cagcattctt
    11341 attgttgata attctgtagc tcaaccatcg aatgtgggac attataataa aaagttattt
    11401 gaagaataca cttcggttga acaggtattt gacagtgctt caattttctg taccgcgagt
    11461 ttgcgtgagt cgctcgaaga aaaaggcttt ttcaaaaacg ttcatatggc aaaaaaccgt
    11521 acaaacctga cagaaaaacc atataaaata accccattga ccagtgaagc ggtaaaaagt
    11581 ctgtgtgctg tgtatcaagc tgatgccatt ctctcgctcg atcgcatatt gcatatcgat
    11641 aagattactg aaaatatcta tccggatgat ttgcattttt tcagcaatct ggatatccag
    11701 atgggaacag tctggtccat aaattaccca accgacaaac ccacaacata tttacagttt
    11761 gctgatagtt ttacctggga aagcagtaat acgatacgca gcaaagctgg tgataagcta
    11821 ccctcgcgtt ataatgcact tgtcgatgct tctctgctca cgggaacaaa tatagccgat
    11881 cgaatgatcc cgcgttggga aaaggaagac cgctatttct ataatccacg gaataaatac
    11941 atgcgacaag ccatggactc agttgaagtc cgaaactgga aagcagccat agatttatgg
    12001 aaactagcag cagaaaaatc aggttatgcc accaaatacc gcgcgaatta caatattgcc
    12061 attgcaagtg aaattttggg cgatatggaa aatgctatat tatattgtga tttggctatc
    12121 gaaatatatc ctaaggtctt atttgcattt aacattgatg cggaaaacga tatatataaa
    12181 ttaatgagtt ataaagaaga acttcaaaaa agagcgaaag aaataaatct gataaaaaaa
    12241 cagcttggca attaaaattg ttactattta caacaaaacc aatcttacat tatgattcaa
    12301 ccaaatgatg aaaagttgct gcagaaaaaa gggatttcag ctcagcaaat tgaagaacaa
    12361 cttaaaagtt ttaaatctgg ttttccctac ctgataatcc gaagttcagc ttcggtagga
    12421 aatggaatta ttcgtgtcaa cgaaactgaa ctgcagactt atctgaatga atggggaaaa
    12481 tacctgacaa caaatgccag agtattgaag tttgttcctg cttcgggggc tgcaagccgt
    12541 atgttcaagg atttattcag ttttctggaa ggtgacagcg atttacctgt tacgggattt
    12601 gagaaaaagt tctttgatga tatagaaaaa tttgcattct acgatgatct gaatgctgtc
    12661 tgcaaaaaaa atacaggtaa aaacattccc gaacttaaaa aagcgaacag ttacagggaa
    12721 attgtggcta acctgttgct ggagaaaggc ttaaattacg gtgcgttgcc caagggtctg
    12781 ctcaaatttc attcttacaa ggacgataaa cgaactccat tgctcgaaca ccttgttgaa
    12841 ggcgctgtgt atgcctccac catatccggt aatgtaaaca ttcattttac agtttccaaa
    12901 gaacaccggc aattgttcga aaaacatctg ggtgaaaacc tctcaaaata tgaaaaaagg
    12961 tttggtgtga aatataatgt cagtttttcc gaacaaaaac catccaccga taccattgca
    13021 gccgatgctg ataataatcc gtttcgtgag gaaggcgaac ttgtttttcg tcccggtggt
    13081 cacggtgcgc tcatagaaaa tctgaacgat ctggacgcgg atatcatttt tatcaaaaat
    13141 atcgataatg tggttcctga tgcactcaaa gatactacta ttacctataa aaaacttatt
    13201 gccggtgttc tggtacattt tcagcaacag gcatttgcct acctcaaaga actcgaaaac
    13261 gaaaagatat cggatgccaa actcacgata atagcacaat tctgcgagaa gaaactgaat
    13321 aaccacaacg aaaatattaa atcactgagc ggaggctgtt tgcgcgagta tcttatagga
    13381 aaacttaacc gcccgattcg ggtgtgcgga atggtaaaga atgtaggtga gcccggaggc
    13441 ggaccatttc tgactgtcaa ttccgatgga acggtttcac ctcaaatact cgaaagttca
    13501 cagattgata tgaataatac tgccgacaaa gaaaagatgc agaactctac tcatttcaac
    13561 cctgtggatc tggtatgtgg cgtgaaggat tacaaaggcc gtaaatttga tttgttgaaa
    13621 tatgtggata aaaacacggg gttcatctcc tctaaatcta aaaatggcaa agagctgaaa
    13681 gcgcttgaac tgcccggact ttggaacgga gccatgagcg actggaattc catctttgtg
    13741 gaagtgccca tagaaacttt caatccagta aaaacagtga atgatctctt aagaaaagaa
    13801 catcagtaag atatataaat aacaatgaaa aaaggatgtt ttcaaacgaa catccttttt
    13861 ttataatatt atcaggtaaa cagtcttaat gtctcctttt catctaagca agttgtcttg
    13921 tacgtataac tatatcatta atttaacgtg agttcgacat aagagaaaaa tatttgaatg
    13981 cagaaaaaat gctgaacatt aataacgtaa taaggttttt ctaaatttta gctatttcaa
    14041 aggtttcctt tcttttataa aaccatattt tcaaaggaat aaacatggac agaactgtaa
    14101 ctgcacatga tctcaacctt ataaaccata tttacagatt aatatgaaat ttgttatatc
    14161 gaactcacgt tatctaaata ataagcagtt ttttcttcaa atgtgcattt acaattggaa
    14221 tttcttttta aaaaaaacta catttgcagc attaatcaac taattatagt cctttaatac
    14281 aaatcataca tggcatccga tgaaaaggaa gaaatagaaa tgttaaagaa tgcttcggct
    14341 gaatcatttg aaactcttta ctttcgatat agtgcaagac tttacaattt tgttttaaag
    14401 ttatcaaaag gcaataactt tatggcagaa gaacttgttc aacgaacatt tattaaagtt
    14461 tgggaaaaaa gagatcagat taatcctgag caatctttta tttcttattt atgtactatt
    14521 gccaaaaata tgctcataaa tgagtacgaa catcaaacgt tagaatttat ttatcataag
    14581 tatttcatag aacaaatatc cttactagaa caccatatta cagaacttga tgttgacaaa
    14641 aaacttctgg atgaatatat tgaaaagcta acggctcagt tacctccaaa acgccgtgaa
    14701 atatttatac taagtaagcg aaatggcttg tcaaataaac tgatttcaga aaaattaaaa
    14761 atctcagaga gtactattga aactcaattg tcaaaagctt tgaaatatat gaaagaacaa
    14821 ctaagattac ttaatgagtg tgtatcttga tttaatttta tccttgttaa aaaatagtaa
    14881 atacaaaaca ctaagtagtg gaaaatcata aactgcctgt atatctaatg tactgatcaa
    14941 ttaatatatg aaaaaggaag aaattctcca attatttgaa cgttatattc tcaacactgc
    15001 atcacccgat gaagtaaaaa aactgtgtca ctgggtgaat aataatcttg aaatttcgaa
    15061 ttggcttgaa aatcaaattc tacattcatc tgatgaaatc gacaaagagc ttcaattaag
    15121 aatgtttaaa gacataaaat cttcactcca aattgaaaaa cccattgaga tgcccacagt
    15181 tgagctaaag gaactaacca gaccttccat tcaaagattt aaaacatgga tgctcgttgc
    15241 gtcattcctt atctttccat taatatccgg actgggtgtt tatctgttta tgagtaataa
    15301 aaaggtttca gatccttcaa ttgtatctgt ggaacgtgga caaaaagcat ctatcacttt
    15361 accggatggc agtaaggttt ggcttaattc tctttcgaag ctgacttact cctccgattt
    15421 taatttagac aagagagagt tattgcttga tggtgaagcg tactttgagg tagctaaaaa
    15481 tccgaagaaa ccattcgttg taaaaagcaa tgaaataact gttgaagcat tgggaactgc
    15541 attcggaata aaagcgtatt cagaagatgt gcgtgtatcg tcaatactta tgcatggaaa
    15601 agtaagagtt acaactccca atggtataaa tattctcaaa ccaaatgaac gaattcagta
    15661 cgataaaata aagcgtacaa cacaacaaag tacagtaact aatgctattg attttacagg
    15721 ttggatacat aacgagttac gtttcgaaaa cgagtcactt gatgaaattg cgaaaaatgt
    15781 acagagaatc tacaatgttg aaataatttt tgcatcagaa ggactaaaga aactacgata
    15841 caccggtacg gtggataata atagtctcga aagcgtatta aatataataa cactgacatc
    15901 gccaatatcg tatcaaatgg acaaacaaaa agttattttg tatgagaaca aaagcatgat
    15961 gaagcattat aatcggcatc aataaataag tgcttattta tatgtacatt acagcaggtt
    16021 gatcttgaat ttcaatctgc tgtttctgtt taattaaaaa aaatttactt tagagtagtg
    16081 gataatattt gttgaactgt atacttaata aaataccaga aattataata tttaattaaa
    16141 ttaataatgg aaagaaaaaa ctacacccat cttttaaggg aaaagtactt aaaactttgt
    16201 ttattcattt tcttatccct tttctcacta actctgactg cacaaataac tatcaacgtg
    16261 caaaacaagc ccattaaaga agtcttaaaa gtaattgaat caaagagcaa ttatcgggtt
    16321 ttttacaatg aagaacttaa tggacttgat aaactggtgt cattacaggt taataatgct
    16381 ggaattgaag aaacgatgag gttaattctg cttaattcag aaatatctta caagctggaa
    16441 ggcaataatc tgattgttct catggctaag gtgaaagaaa atcaaggttt gtcaaaaaag
    16501 attactggtg tagtcaccga tataaatgga gattacattc taggtgcatc tgttttaatt
    16561 aaagggtcta aaacgggcgc tctaaccaat atgaaaggac agttttcttt agatgtttcc
    16621 gacaattcat tgctgattat ctcttacatt ggctataaga cgcaagaaat ttcagttgct
    16681 ggtaaaaatt caattcaggt taagctgatt gaagatgtag aactactaaa tgaagttgta
    16741 gttacggccc ttggtatcaa gagggaagag aaagctctgg gatatgctgt tcaggcagtc
    16801 aatggcgaga aactacaaac ggtaaaaggg gttgatatgg ctacttctct aaccggcaaa
    16861 gttgcaggat tgatggtgaa aaacagtact gaatttgcac aatcgcctga tattcaaatt
    16921 cgtggtgaga atcctctgat tgtaattgat ggagtgcctt atggtaatat gacactgaaa
    16981 gatattccct ctgatgatat tgaaaatttg agtgtgctta aaggtgcaac tgcttcagct
    17041 ttatatggtt atcgtggtgc cggtggtgct atcatggtta ctacaaaaaa aggaaataag
    17101 aacaaaggtc tggaagtgtc tcttaatagc ggatcaatgt ttacagcagg tttcctggct
    17161 attcctgaat tacaatcaac atttggacgt gttgttaata ccgctacaaa tacttactcc
    17221 accggtggag atggttcatg gggcattccg atggatggtc gtgaagtaaa gcaatgggat
    17281 ccggtaacta aaacatggct tttaatgcca tatctaccca gagggaaaaa caactttaaa
    17341 aacttccttg aacaaggtta tgtattaaac aacaatgtga atatagttca acagggcgag
    17401 tttggaagtt tccgggcatc ggcaacctgg gtaaacaata aaggtcagta tcctaattca
    17461 gattacaata agtacaccta cggtattggt ggtgatatga aattaaataa gttccttttg
    17521 tcatcaagta tttcttataa caaacagacg tcccctaata taggtttcag tggttatacc
    17581 agctatgacc caatgtacag tatgttggtg tggtctgctg ctgactacga tgttagggac
    17641 tatatggact attggttaat accaaacgaa tcgcaaaata acagttatac cgattcaagt
    17701 aataacccct atttcgatag gttccaaaga actcatagca tcaataaaga tattatgaat
    17761 ggaaatttat cggtaaatta tgactttaca ccgtggttga aattatcgtt aagatcaggt
    17821 tttgatactt acagtgatcg tcaggacatc aagatttcta aagggtcatt aattagtgca
    17881 ggttcagcca cattaattcc taatggcaca caagtttggg gtgaatcggc caatggatcc
    17941 tataatactg gaataagtcg cggttatagt tttaataatg atctaatctt atcaggtagt
    18001 aaaacattcg ataaatttag tgttgatggt ttgtttggag gtacgatttt ttacaaacaa
    18061 gatgagggta ttgaggctaa aacacaagat ggacttacca ttccgggata ttattcatta
    18121 aatgcttctg ttagtcctgc caatgttagt tctactattt atcgtcgtca ggttaatagc
    18181 ttgtacggtc gtctggcaat gtcatggaat aatctaatat atgctgaagg cactatgcga
    18241 aacgactgga gttcaacctt gtcgcagtca acccgttctt atctttatcc atcagtttcg
    18301 ggcagcttta ttgcatcgga attattacca aaaactgatt ggctttcgtt atggaaaatc
    18361 cgcgggtcat ggactacatc aaagacaccg gcagatgtat atgccattaa ttctgtctat
    18421 acaattacaa atagtgcatg gggaagtttt agtacggcat catatcccgg gagtatacgc
    18481 agttcggttg taatgccgga atcttcagag acttttgaag tgggcactgc cattaattta
    18541 ttaaaaaaca gagcatcttt agatttctcc tattattcaa aaaggatgta cgacggactg
    18601 acatggggag gaataagttc ggcctcaggt tttaccagta attacgtaaa tacagatgaa
    18661 gaagttacca gaagaggtat tgaaataact gcaaatgtaa cgccggtaaa aacaaaagac
    18721 tggcagtggg atctatctgt taactggtcg aaatatgcac gatatttcac aaaactggac
    18781 tctttgtact cctcagataa accctgggtt aaagtcggag aacgttatga tgcatatgtg
    18841 ctgtatgatt atttacgtga tcctgatggg aatattattc ataatgcagg caaacctctc
    18901 tactcagatt atacctcaaa atttggctat tctgatcctg actggatttg gggtctaagt
    18961 tcttctgtaa gatataagaa tttccagttt tctgtctcaa tggatggacg ggttggtggc
    19021 cttactcaga caataacaga aatgtatatg tggcgagccg gatctcatcc aaactcagtt
    19081 acagatcaaa gatatctgga tgcaacagtt ggaggaaata attatattgg cgaaggagta
    19141 aaagttgttt caggttctgc aacgtacgac acatatggca acataacaag tgatacaagg
    19201 gtatacgcac cgaatgatgt tgcaactact tatgaaactt atataaatgc attacataaa
    19261 ggtaccgcct ggggtggagc tgcatcacct attgatgttt actctacaac ctttttaaaa
    19321 atccgcgaga tgtcattaac atacacgctg ccaaaacata tttgtgaaaa aatatatacc
    19381 aagggaattt ctttgtctac cgttggtcag aatttatttt tatggtcaaa acagtttaag
    19441 tattctgatc cggatggagg atatgataac tttagtgacc cctctattcg atatattggg
    19501 tttaatgtta aagttagttt ttgataattt gaaaattaaa tcaattccag aaacatataa
    19561 ttaatagttt aaaatttaat acaattacaa atgaaaaaac taaata
//
LOCUS       DAOPYB010000038        19509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2912_length_19509_cov_9.255629, whole genome shotgun sequence.
ACCESSION   DAOPYB010000038 DAOPYB010000000
VERSION     DAOPYB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19509
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2912_length_19509_cov_9.255629"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..891
                     /locus_tag="PKH58_04695"
     CDS             172..891
                     /locus_tag="PKH58_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX88357.1"
                     /translation="MKIQLFIPCFIDQLYPQTGFNTIKLLEKAGCEVLYNPNQTCCGQ
                     PAFNSGYWDEAAALAHKFLNDFDPALPVVSPSGSCTGFVIHHYHKLLKDQPELMGKLD
                     KQKQHIYELSDFLVNVLHYTDFEACFQHKVTYHDSCSALREYGIKNEPRQLLSRVKGL
                     ELIEMEECETCCGFGGTFAVKNPDISSAMAEKKVLNALATGVEYIVSTEASCLMNING
                     YIAKHKLPMKGIHLADILSSF"
     gene            complement(918..989)
                     /locus_tag="PKH58_04700"
     tRNA            complement(918..989)
                     /locus_tag="PKH58_04700"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(954..956),aa:Glu,seq:ctc)
     gene            1163..2599
                     /gene="amrB"
                     /locus_tag="PKH58_04705"
     CDS             1163..2599
                     /gene="amrB"
                     /locus_tag="PKH58_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNX88358.1"
                     /translation="MITDISNSDRYPAVAGQFYPADAVKLELELSRLFDEATPKKHEQ
                     VRAVIAPHAGYVFSGRIAASSFSQIDTSVTYRRIFILGSSHHFRFEGASVYCGGDFIM
                     PYGTEKVDTELGLELTKKHPTLFSDYRDAHFKDHSIEIQLPFLYYQLKGNYKIVPIIL
                     GTAYPDICREIAMALKPYLTHDNLFVISTDFSHYPQYNDALQADEATKNAILTGKPNV
                     LRTSLADLAQKNIPNLLTGLCGWTSVLTLMYMTENEKQYSYEEVEYCNSGDIRHYGER
                     NQVVGYWSIILKENKSAEKKEFSLTDAEKKQLLHEARQSIKHLFDKTVLSKNEIVSET
                     LQTNCGAFVTLHEHKKLRGCIGMVVAHKPLIDTVREMAVSAARHDYRFQPVTEEELDD
                     IEIEISVLSPLKHIQDIREIIPGVHGIFIQKGRQSGLFLPQVTTETGWNLEEFLGHCA
                     RDKAGIGWDGWKEADIYTFTATVFQEEK"
     gene            complement(2600..3625)
                     /gene="amrS"
                     /locus_tag="PKH58_04710"
     CDS             complement(2600..3625)
                     /gene="amrS"
                     /locus_tag="PKH58_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNX88359.1"
                     /translation="MNADRFQILSRYQEKTEGNRVRCHICPHNCLIPEGKQGNCHTRI
                     NREGTLWSMAYGNPCSVSIDPIEKKPLFHFLPGENIFSLATAGCNFRCLNCQNWQISQ
                     SAPSEVKSYDLSPEEIVQQAESSGTRLIAFTYTEPTVFYEYMFDTASIAHERSLKTVV
                     VSNGYINPDPLLELIPFLDGANIDLKCFDNDTYLKLTGGKLQPVLDTLITLKKKNVWL
                     EITNLLIPAFNDNPKQIKAMCSWLADNGFTETPLHFSRFFPNYRLQDAAPTSVKLLLQ
                     AKQIAEDRGIKYVYLGNIPQLSGENTICSHCGKTLIIREGFSVKENQLSKGKCRYCET
                     AIPGVWE"
     gene            complement(3640..4569)
                     /locus_tag="PKH58_04715"
     CDS             complement(3640..4569)
                     /locus_tag="PKH58_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNX88360.1"
                     /translation="MKKICAVILLMVFVSGIQIHAQKTDKKLTKLIENEVSDFNGVAG
                     VYVKNLKTGREVTLNADTLFPTASTIKVPIMVGVFNKIINRQLDYYQPLTYSIQKQLY
                     AGDDGLIASLRDSSKVSLSIVQMLSITTSDNSASLWLQELAGTGTEINNLMDNIGLKH
                     TRVNSRTPGREAIREIYGWGHTTPREMAGLLVKIRNREILTPAACDEMYRILGTIYYD
                     DRALSQIPPTVKTASKQGMVSDARSEVVLVNAPHGDYVFAVYTKNQKDLSWKKNNEGR
                     QLIVEISKLLWNYFEPGFGWKPAEGNDEYNGAD"
     gene            complement(4606..5715)
                     /locus_tag="PKH58_04720"
     CDS             complement(4606..5715)
                     /locus_tag="PKH58_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX88361.1"
                     /translation="MEDKLVTLAIRTYQRAQMIKSVLEQNGIETQIHNLNLEHPEMAV
                     GVRVRIKESDLPRALAIVEEKEKDWELEETKDEPPKGRKIIIPIDLTDQINEVCLYGF
                     QFAEKLRAEVIFLYAYFTPAFTISSNNEISTYSLADSEMLRRIVNTNYADVENITNLI
                     NKWISAGIVPDVKFRFELKEGVPEDQILEMCKKEKPVMVVMGSRGKKKNDEEIIGSVT
                     AEVLEACNVPVFAVPMNLNIKSPADIKRIAFLTNFDQKDLIAIDTAISFFDREKLELI
                     FIHASQKKDTWDEVMLAGIKEYFSKHYEGIKTEYAILNREENLNQIVQFLEETDIDLV
                     ALNTKKRNIFARFFNQGIATKLLFNVDMPLMVMHM"
     gene            5928..6329
                     /locus_tag="PKH58_04725"
     CDS             5928..6329
                     /locus_tag="PKH58_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88362.1"
                     /translation="MKKAIWIISLIVVLVLSGIVYYKYYFVFGEGVKAGELNYMVKKG
                     VIFKTYEGKLIQSGFRSSGKTPGPTIQSYEFEFSVENKGIADTLMLSSGKDMELHYKE
                     YNGVLPWRGFSKYVVDKIIKIENKEPSTIPF"
     gene            6476..7636
                     /locus_tag="PKH58_04730"
     CDS             6476..7636
                     /locus_tag="PKH58_04730"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNX88363.1"
                     /translation="MEVSELKRSFKEAFGLEADAVYFSPGRVNLIGEHTDYNGGFVFP
                     CALSFGTYLLIRKNGEKFVQFRSLNQPEVVKVTLDKLTERLEKSWANYPLGVFAQFVK
                     RGYTFEQGFDIMIWGDVPNGAGLSSSAALEVVTAYALNDQLGTGLNRTELAKIGQKAE
                     HEFALVNCGIMDQFASANGKKDHAIFLNCDTLDFELVPVKLDGVKVVISNTHSPHKLD
                     SGAYNQRVAECKKAVEQLNTVRSIKYLAELTESEFEKIENAITDPVAHKRARHVVGEV
                     QRTTDAVKALKEGDIELFGKLMNASHVSLRDDYEVTGPELDTMAEEAWKIEGVIGSRM
                     TGGGFGGCTVSLVKDEAIEEFKEKVGKAYEAKINIKPDFYIAEIGNGAMKVE"
     gene            7722..8750
                     /locus_tag="PKH58_04735"
     CDS             7722..8750
                     /locus_tag="PKH58_04735"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNX88364.1"
                     /translation="MAEEIIIEGKKVSLYEIKNNKGMSAVITNYGARVVKLWVPDRNG
                     ELADVVLSFDTLEEITTKETYFNSTCGRFANRIKDGKFTLDGKEYSLAINNGPNHLHG
                     GLVGFNMRVWEVKSTTDNAIVLYYFSPDGEEGYPGNMDVTVTYELTDDNELKIHYEAT
                     TDKPTIAGFTNHSYFNLKGAGEGTVRDHILQLNADFHTALDDNTCPTGEVLKVDGTPY
                     DFREPTLIEERIDNPVYASNRGLDDNWVIRRNQPHELALAGYIYEPKSGRKMEVLTTK
                     PGVQIYAANWVEKQTGKYGKQYDAQCAVCLEAQGFPASPVYPHFPSPVLRPGQKYDEW
                     CIYKFSVE"
     gene            complement(8792..9814)
                     /locus_tag="PKH58_04740"
     CDS             complement(8792..9814)
                     /locus_tag="PKH58_04740"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX88365.1"
                     /translation="MILVTGATGMVGGHLIWYLLQSNGRISALTRSNSNTTDLRTVFE
                     FYTNTPDQYLSRVDWVKGDVLNFNSLKRAFEGMEYIYHCAAIVNLGKNYNEMILTNAK
                     GTENVVNAALCNKVKKLCFVSSIAAVSDDISHGPIDENTPIEPDKPVSTYSESKMLAE
                     KEIEKGIQKGLNAVIVNPGVILGISKKMTGSGELFKLAKKGLPFYTFGKTAYVDVRDV
                     CKAMILLMNSNINGERFIIAGENCYHRDILRWMAEGYHTYRPFIGFGKLLIPLAGVLE
                     IIGKIAGFTPLIDRSSASISISRKEYSCKKFLSRFEFTFTPIRECILEICDFDLKNQE
                     KTVLHP"
     gene            9901..10479
                     /locus_tag="PKH58_04745"
     CDS             9901..10479
                     /locus_tag="PKH58_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNX88366.1"
                     /translation="MQNICVYCASSSKIDSKYFETARELGAILAKNDLRLIYGGGSVG
                     VMGVLANSVLEDGGKVTGVIPQFMCDAEWQHDGLTELVITETMHERKEKMALLGDAAV
                     ALPGGCGTLEELLEIITWKQLGIYTKPIVIVNVNGYYNHLIAQFQQAADELFMRPQHL
                     QMWTVVDSAGQILDAIANAYSWDENLRQYAKI"
     gene            10507..11319
                     /locus_tag="PKH58_04750"
     CDS             10507..11319
                     /locus_tag="PKH58_04750"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNX88367.1"
                     /translation="MPASDSIIKLVDTTARLTTDTLAVSDSLSKADSIAGIDSIKLAA
                     SLPPSGMEGIKLSSLPANESWVFIVLLVLFLFYVGSLLKSGGEFLQNLRSTFKHNYTW
                     GTSSVSTLNYLQSRILIILCSVGIFSLLIQEFLFVMPGNFELKTFFLSVGVTSGFYLL
                     KLLLFQMVGNVFFDKKTVLNYQNIYFNFLSLFGIVLLPVLILYTYQPLSWKLPLEIIT
                     GLLLGCFYVLLIIKLCQVFYTKKLALFYIFLYLCTLEILPLLVLFRVYELVI"
     gene            11346..12107
                     /locus_tag="PKH58_04755"
     CDS             11346..12107
                     /locus_tag="PKH58_04755"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNX88368.1"
                     /translation="MKIKKILVSQPRPTTEKSPYFDIIDKYSVKIDFRPFIKVEPVTA
                     REFRSQHVNILDHTAIIFNARHGIDHFFRLCEEMRITVPETMKYFCVSETVALYLQKY
                     IQYRKRKIFFSPTGKFPDLITVLNKHHEEKFLYITSDIRNEETMSILENSKVSFSQGI
                     MYRTVSNNFAPDEPFDYDMLIFFSPAGIKSLLENFPNFEQGEIQIGCFGAITAQAVRD
                     SGLRLDLEVPSPGVPSMTMALDNYLKEAKKNNGKK"
     gene            12199..12606
                     /gene="rnpA"
                     /locus_tag="PKH58_04760"
     CDS             12199..12606
                     /gene="rnpA"
                     /locus_tag="PKH58_04760"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNX88369.1"
                     /translation="MNTFPKKEHLCGEKIITRLHQQGRAFIAFPYRVVYLVADENETE
                     PVRVMISVPKKRFKHAVDRNRIKRLTREVYRLNKHEITKFALEHNLKFHLSFQYVSNE
                     EESFEMLEKKMKLALKKLILNFSEPNCKNETTP"
     gene            12590..12844
                     /gene="yidD"
                     /locus_tag="PKH58_04765"
     CDS             12590..12844
                     /gene="yidD"
                     /locus_tag="PKH58_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNX88370.1"
                     /translation="MKQLLKILEFIVLIPIYFYRYAISPLKHPSCRYTPTCSEYAIQA
                     IKKHGILKGIWLGTHRILSCNPWGGSGYDPVPEKFSFKKK"
     gene            12971..13651
                     /locus_tag="PKH58_04770"
     CDS             12971..13651
                     /locus_tag="PKH58_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNX88371.1"
                     /translation="MKKQVSVAFMASGLLFTTCLIVANIVEQKLISIGPIEATAGLLI
                     FPVSYIVNDIIAEVWGYRKARLIIWYGFLMNFLAVVIFRLSIIVPGSENFTHQDAFQL
                     VLGNTERISLASFIAFLIGSFLNAMVMSKMKLLQKGRNFSLRAVVSTLVGEGADSLVF
                     FTIAFAGVIPNNDLLMLILTQAGMKTAYEILVLPLTNVIVRWVKRREQEDVFDDGISY
                     NPLKINEI"
     gene            complement(13654..14286)
                     /locus_tag="PKH58_04775"
     CDS             complement(13654..14286)
                     /locus_tag="PKH58_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HNX88372.1"
                     /translation="MIQAASDTLSEKSYSGINTCDTENNVIFPEFKLRNQDAPIGKKL
                     VRGVLINSAVNATMITALVLSPKEFSMWTDDDRLKWYMIRQRFKNAYTNPPVIDHDLF
                     FTNYVGHPYQGSLYFNTIRSQGSTFLESSLYNLAQSAAWEYVWEAGMEQPSIQDLIVT
                     PIVGTVLGELFHKATLGMRKNGFNWYEKVIVCIINPYYAVNNGLSTRRSR"
     gene            complement(14606..16141)
                     /locus_tag="PKH58_04780"
     CDS             complement(14606..16141)
                     /locus_tag="PKH58_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX88373.1"
                     /translation="MKKFLLFVLLAFSANAIFANQLLPGTARKPVKSPLEIVKSFPKK
                     STWKTPGVLTTEKINEYIKAQQALAIQYELLDSVRYWNWDVNTKGWVNDHKTIKITYD
                     NKDRIISSIEQEWNNDTQEWENTSKSTYTYNSSDLMTMLSIETWDGSNWTNGMKTTIT
                     YDENKNSTTTKLEMWFMTDWTTISLSTFTYDANHNVLTEVNQSGMFGPLANVSKEIFT
                     YDAKNKLLTSTQQTWETNQWVNSSYLVNTYNNHELITSVTTQKWEANKWVNESKHSYT
                     YDANDNNTNLLYQNWNNGNWDNSQQWISTFDTRNNETTQTTQVWDAGAWKNQYKTTYT
                     YNASDDVATELHQEWTENTWVNYKQDTYTYDANGNVTRDTEKTWLNNSWVLSDLMLGT
                     YDSNNYPMTESDKSYDQDGITVSSGDSVRYYYRPGTDVQSLSSERFNVYPNPNKGKFT
                     VSCSEKIDGLEIFNSTGEIIFSTLKADDRKKSIDLSNYSKGIYVIRINSGNKLLTKKI
                     ILQ"
     gene            complement(16241..17011)
                     /locus_tag="PKH58_04785"
     CDS             complement(16241..17011)
                     /locus_tag="PKH58_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNX88374.1"
                     /translation="MKQKFFLFLLAISLTLPAMAYDAFGHRIIAGIAYENLTCHARKK
                     VDKLLGKHGLIYESTWSDDVRSDSAYAYSYNWHFQNLRDGMTPTDILSLYEKPKTEGL
                     HLFFAIDSLSQSLKNNKKDIEALKFLIHFIADAHQPMHLGRAGDRGGNDIMIKWFGKD
                     IRLHQLWDTQMIEGRNYSYSEYVEFLQDKFAGRNNEFKKQSLPQSLEAVYQVRNKIYK
                     YDYNNLRAYNYMYVFNNDLDELLYRGGIQLANLLNKVM"
     gene            17267..18163
                     /gene="xerD"
                     /locus_tag="PKH58_04790"
     CDS             17267..18163
                     /gene="xerD"
                     /locus_tag="PKH58_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNX88375.1"
                     /translation="MLSILDEYHIYLKMEKSLSENTIEGYERDLKKLFSYLADAHILP
                     EEAEDRHLRDFIIETGEIGINERSLARLISSIKSFYKFLIYNDKITIDPTELIQSPKI
                     GLRLPDVLTLSEIDRIIEAVDLSKPEGHRNRAIIEVLYGSGLRVSELINLKISNIYFN
                     DGYMLVEGKGNKQRLVPISDESKKQIDFWVNDRNHLNIKKGHEDYLFLNRRGSRLTRM
                     MIFAIVKELAVIAGIHKNVSPHTFRHSFATHLLENGANLRAIQQLLGHESITTTELYT
                     HIDVQFLRKTILEFHPMNQKKN"
     gene            18165..>19509
                     /locus_tag="PKH58_04795"
     CDS             18165..>19509
                     /locus_tag="PKH58_04795"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNX88376.1"
                     /translation="MAIRASFSPAIKRRIVFLCVLFLSAFIHLAAQETVVVGQVIDKY
                     DQSPIASVDIYFKNSNVAVQSNDEGYFLIRNTGPETTLIFTVIGYKSYELKLKRGDNV
                     GVQIELEEKKNILDEVLIIPGVNPAVDLMKKVRQKRRQNDVKVLLSATDQSAVFLSKN
                     DARWQNNKLFEQLKSGNLSENDSVLLMPIYMVESEYDAKGLVKRQKNKKTYNTDETTE
                     NLVDKLLDGMDNRISFYDNSVNLLGKNMISPLASIGSTFYRYYLTDSILTPTGKQYDI
                     HFYSRNPKSLAFNGSMYVDSATLALTYISASLPRQANLNFIHNLSFVQKFKKTENYWI
                     PDNQQSTWYLTYDLLKNDAGASSELLVSRNTSYSSGEGDIVLQTDSFAGSAFSDKEIN
                     TRMIALQQSKIFKAASIIADAGLTGYLKAGKIDVGLLATILRLTEEEGLRMGLNMR"
BASE COUNT         5665 a         3838 c         4031 g         5975 t
ORIGIN      
        1 aaacgaacat acaacggcat gacctatcag ggcggatttg ccgatcactt acccgttttt
       61 gtggatttgt ggtattaaaa ccattctaag tacattatga ttgaatgaat tgaagaataa
      121 aatatatttc agatagtttg ttacgctctg aatgatgaaa aaagaacatt aatgaaaata
      181 cagcttttta ttccctgttt tattgatcag ctttatcccc aaaccggttt caacaccatc
      241 aaactgttgg aaaaagccgg ttgtgaggtg ctatacaatc ctaatcagac ctgttgtggt
      301 caacctgctt tcaacagtgg atactgggac gaagcagctg cccttgctca caaattcctg
      361 aatgattttg atcctgctct gccggttgtt tcaccttcgg gctcctgcac cggcttcgtc
      421 attcatcatt atcacaaact cctgaaagat cagccggaat taatgggcaa gcttgacaaa
      481 cagaaacaac acatctacga actttcagat tttttggtaa atgtgcttca ttacaccgac
      541 tttgaggctt gttttcagca taaagttact tatcacgatt cctgctcggc gcttcgggaa
      601 tatggaatca agaatgagcc acgccagttg ttaagcaggg taaaaggtct cgaactgata
      661 gagatggaag agtgtgagac ctgttgcgga tttggaggaa cttttgcggt aaaaaatccc
      721 gacatatcat cggctatggc cgaaaagaaa gtgcttaatg cgctggcaac tggtgtggaa
      781 tacatcgtta gtacggaagc ttcctgtctg atgaatataa acggttatat cgccaagcat
      841 aaactaccaa tgaagggcat tcatcttgcc gatattcttt ccagtttttg acttttttca
      901 gtggaaaagt ataaaggtag tgcgtagcag aatcgaactg ccattgtcgg attgagaaac
      961 cgatctccta accgttagag gaacgcaccg ttttatacca tattgatgaa tgcaaaatta
     1021 attctttttt cagaaaactg taattatttt ttttatttct gaaattaatt catcagccat
     1081 gttctttctt cacagattat catatcattt caaacatatc tgccattcgt actgttttat
     1141 ttatggtaaa taatatggag ttatgataac ggatatttca aattccgaca gatatccggc
     1201 cgtagccgga caattttatc ccgcggatgc cgtcaaactt gaattggaac tgtcgcggct
     1261 attcgatgaa gctacaccca aaaagcatga acaggtacgg gctgttattg cgccacatgc
     1321 cggttatgtt ttctcgggta ggatagctgc ttcttctttc agtcagattg acacttcggt
     1381 tacttaccgg cggattttta ttcttggctc atcgcatcat ttccggttcg aaggcgcatc
     1441 ggtttactgc ggaggtgatt ttatcatgcc ttatggcaca gaaaaagttg atacagaact
     1501 gggactggag ctgaccaaaa aacatcctac ccttttttcc gactacagag atgcacactt
     1561 caaagatcat agcattgaga tacagcttcc ttttttgtat tatcagctga aaggaaatta
     1621 taaaatcgtt ccgattattc tcggaactgc ttatcccgat atttgtcgtg aaatagcaat
     1681 ggcattgaag ccatatctga cccatgataa tttgtttgtg atcagcaccg atttctcgca
     1741 ttatccacag tacaacgatg cgctgcaggc ggacgaagcc accaaaaatg ccatcttgac
     1801 cggtaaaccg aatgtgttaa gaaccagttt ggccgatctt gcacaaaaaa atatccccaa
     1861 tctgctcact ggattgtgtg gctggacttc ggtactgacc ctgatgtata tgaccgaaaa
     1921 cgagaagcag tatagttatg aagaagtaga atattgcaat tcgggtgata tacgacatta
     1981 cggggaacga aatcaggtgg tgggttattg gtcaataatt ctcaaagaaa ataaatctgc
     2041 cgaaaaaaaa gaattttcac tgactgatgc ggaaaaaaag cagctgctac acgaagcccg
     2101 ccaatccatt aaacacctgt tcgataagac cgttttatcc aaaaatgaaa ttgtttctga
     2161 gaccttacaa acaaattgcg gggcttttgt aaccctgcat gagcataaaa agctccgtgg
     2221 ctgcatcggg atggtcgtcg ctcataaacc gctgattgat acggtacgcg aaatggcagt
     2281 ttcggcagcc agacacgatt accgttttca gccagtgact gaagaggagt tggacgatat
     2341 tgaaatagaa atttcggttc tttcgccact caaacatata caggatattc gggaaatcat
     2401 tcccggagtt catggcatct tcattcagaa aggaagacaa agcggattat ttttgcccca
     2461 ggtaactacc gaaactggct ggaacctcga agaatttctg ggacactgcg cccgcgataa
     2521 agcaggtatt ggctgggacg ggtggaaaga ggcagatatt tacaccttta ctgccacagt
     2581 ttttcaggaa gaaaagtaat tattcccaca caccgggaat tgctgtttca caataacgac
     2641 atttaccttt cgataattga ttttccttca cagagaaccc ttccctgata ataagggttt
     2701 ttccgcagtg ggaacatatg gtattttcgc ctgacagttg cggtatgttt ccgagataaa
     2761 cgtattttat tccacggtct tcagctattt gcttagcctg caacagaagt ttcacagagg
     2821 tgggcgctgc atcctgcaac cggtaattag gaaaaaaacg gctgaaatga agcggtgtct
     2881 cagtgaaccc attgtcagcc agccaggagc acatggcttt gatttgtttg ggattgtcat
     2941 tgaaagccgg aatcagtaag ttggttattt caagccatac atttttcttt tttaatgtga
     3001 tcagtgtgtc gagcacaggt tgaagtttac cgccggtcag tttcagataa gtatcattgt
     3061 caaaacattt caggtcgata tttgcaccat ccagaaaggg gattaactca agcagcggat
     3121 ccggattgat gtaaccgttg gaaacgacga cagttttcaa acttctctca tgcgcaatac
     3181 tcgctgtgtc gaacatgtat tcgtagaaaa cggttggttc cgtgtaagtg aaagcgatca
     3241 accgtgtacc cgatgattca gcctgttgaa cgatctcttc aggacttaaa tcataactct
     3301 ttacttcgct gggtgcggat tgagatattt gccagttttg acaattaaga cagcgaaaat
     3361 tacatccggc tgtagccagc gaaaaaatat tttctccggg tagaaagtga aataatggtt
     3421 ttttttcaat cggatcgata cttacagagc aaggattacc gtaagccata ctccaaagag
     3481 taccttcacg attaatccgt gtgtgacaat tcccttgttt tccctccggt atcaggcagt
     3541 tgtgcggaca aatatggcat ctgaccctgt ttccttcggt tttctcctga tatcgggata
     3601 atatttgaaa ccgatccgca ttcatgacac cttatttttt taatcggcac cgttatattc
     3661 atcgttacct tcagcaggtt tccacccaaa gccgggttcg aagtaattcc agagcaattt
     3721 tgaaatttct acaatcagtt gccggccttc gttgtttttt ttccacgaca ggtctttctg
     3781 gtttttggta taaacggcaa atacgtaatc gccatgaggt gcattgacga gtacgacttc
     3841 agatcgtgca tcgctcacca ttccctgttt tgaggcagtt tttacagttg gcggtatctg
     3901 gctcagtgcc cggtcatcgt aatagattgt acccaaaatc cggtacattt cgtcgcaagc
     3961 tgccggtgta agtatttccc tgttgcgtat ttttaccagt aagccagcca tctcgcgtgg
     4021 agtggtatgc ccccagccat agatttcccg gatggcttcc cgtccgggtg tccgtgaatt
     4081 tacccgggta tgtttcagcc cgatgttatc catcagattg ttgatttcag ttccggtacc
     4141 ggcaagttcc tgcagccaca ggctggcgct gttgtcgctg gttgtgatac tgagcatctg
     4201 tacaatgctt agcgaaactt ttgaactgtc gcgaagtgat gcaatcagtc catcatctcc
     4261 ggcgtagagt tgtttttgta tagaataagt aagaggttga taataatcca gttgcctgtt
     4321 gataatttta ttgaaaacgc caaccattat cggaacttta atggtgctgg cagttgggaa
     4381 aagcgtatcg gcattcagtg tgacttctct gccggttttc agatttttta cataaactcc
     4441 ggccactcca ttaaagtcgc tgacttcgtt ttctattagt tttgtgagtt tcttatcggt
     4501 tttttgagca tgaatttgta tccctgaaac aaagaccatc aacaaaatta cagcgcagat
     4561 ttttttcatt atatatttga atttgataat atttttacaa aaaacttaca tatgcattac
     4621 cattaatggc atatccacat tgaaaagcag tttagttgca attccctgat tgaagaaacg
     4681 ggcaaatatg ttacgttttt ttgtgttcaa agccaccaga tcaatatctg tttcttcgag
     4741 gaattgaacg atctggttta aattttcttc ccggttgagg atggcgtatt cggttttaat
     4801 tccctcataa tgtttactga aatattcttt gattccggcc aacatcactt catcccacgt
     4861 gtcttttttc tgtgaggcat ggatgaaaat cagttccagt ttttcgcggt cgaaaaatga
     4921 aatggctgta tcaatagcaa tcaaatcttt ctgatcgaaa ttggtaagga aggcaatgcg
     4981 cttaatatcg gcaggagatt ttatgtttaa gttcatcggc actgcaaaaa cgggtacatt
     5041 gcaggcttcc agtacttcgg cggttacgct gccgattatt tcttcgtcat tttttttctt
     5101 gccacgtgat cccatcacca ccataacagg tttttctttt ttgcacattt ccaggatctg
     5161 gtcttcgggt acgccttctt tcagttcgaa gcgaaatttt acgtccggaa caatccccgc
     5221 tgatatccat ttgtttatca ggttggtaat attttccaca tcggcatagt tagtatttac
     5281 gatccgcctg agcatttcac tgtcggcaag actgtaagtg ctaatctcgt tgttggatga
     5341 aattgtaaaa gccggtgtaa aataggcata aagaaaaata acttcggcgc gaagtttttc
     5401 ggcaaactga aatccataca gacatacttc attaatctga tcggtaaggt cgatgggtat
     5461 aataattttt cttcctttgg gcggctcgtc ctttgtttcc tcaagttccc agtctttttc
     5521 tttttcctcg acaatagcca gtgcacgcgg aaggtcgctt tcctttatgc gtacacgaac
     5581 gccaactgcc atttcggggt gttccaggtt cagattgtga atttgagtct ctatgccgtt
     5641 ttgctccaga acgcttttaa tcatttgagc acgctggtaa gtgcgtatgg ctaatgtaac
     5701 tagtttgtct tccatggctg atgtgatgaa atgttaatca tataactgct gaaatgtata
     5761 tatccgtaaa tcaacttatt atttattttc ttcgatggta aaattagcaa gaattaatca
     5821 aaatctgatt agttttttat aaaaaaacaa tcgtgctaag aaaattgcta tatatttgca
     5881 acgaattaac aataaacaga atttatacaa aaaaatattc aggaattatg aaaaaagcaa
     5941 tctggatcat atcgctaata gtagtcctgg ttttatcagg tattgtctat tataagtatt
     6001 attttgtgtt tggagagggt gtaaaagcgg gagaactcaa ttatatggtg aaaaaaggtg
     6061 tcattttcaa aacctacgaa ggaaaactga ttcaatccgg atttcgttca tccggaaaaa
     6121 ccccgggacc gactattcag tcctacgaat ttgagttttc ggtggaaaac aaaggcattg
     6181 ccgacaccct gatgctgagc agcggcaaag atatggagct gcattacaag gagtacaatg
     6241 gtgttctgcc ctggcgcggt ttcagcaaat atgtcgtaga taaaattatt aaaattgaga
     6301 ataaggagcc ctcaaccatc cctttttagc attatcaaaa actccgaaat atattgataa
     6361 ttaacaattg ataattatct cttaacaaaa atatctgcct atttccggta aatttgcaga
     6421 aactaattct ttgtgcaata tctgaaaaat tattcattat aaaatttaat acaacatgga
     6481 agtatctgaa ttgaaaagat cttttaaaga agcttttggt ctggaagctg atgcagttta
     6541 tttctcgccc ggcagggtta acctgattgg agaacatacc gattacaatg gtggttttgt
     6601 gttcccttgc gcgcttagtt tcggaaccta cttattaatc cgtaaaaatg gcgaaaaatt
     6661 tgttcagttc cgttcgctca accagcccga agtcgtaaaa gttactctcg acaagctgac
     6721 tgaacgtctc gaaaaatcat gggcaaatta tccgcttggt gtatttgcac aatttgtaaa
     6781 acgtggatat acgtttgagc agggcttcga tattatgatt tggggcgatg taccaaacgg
     6841 tgccggactt tcatcatctg cagcactcga agtagttaca gcatacgctc tcaacgacca
     6901 gctcggaaca ggtttgaacc gcaccgaact tgcaaaaatt ggtcagaaag ctgaacatga
     6961 gttcgctttg gttaattgcg gtatcatgga tcagtttgca agcgccaatg gtaaaaaaga
     7021 tcatgcaata tttttgaatt gcgatacact cgattttgaa ctcgttcctg tcaaactgga
     7081 tggtgttaaa gtcgtgattt caaatactca cagcccacac aagctcgact caggggctta
     7141 caatcagcgc gtagccgaat gtaaaaaggc tgttgaacaa ctcaatacag ttcgttccat
     7201 caaatatctg gccgaactga ccgaatccga atttgagaaa attgaaaatg ccattaccga
     7261 tccggtagct cacaaacgtg cccgtcacgt cgttggcgaa gttcagcgta ccaccgatgc
     7321 agtgaaagcg ctgaaagaag gtgatattga gctttttggt aagctgatga atgcatctca
     7381 cgtttcactc cgcgacgatt atgaagttac cggtcccgaa cttgatacga tggccgaaga
     7441 agcatggaaa atcgaaggag ttatcggttc acgtatgaca ggtggcggtt ttggcggttg
     7501 cactgtatct ttggtaaagg atgaagccat cgaagaattc aaggaaaaag ttggcaaggc
     7561 ttatgaagct aaaatcaaca tcaaaccaga cttctacatt gctgaaattg gcaacggtgc
     7621 catgaaagtt gaataatcaa acaaaataca atcaattccc tttctgcaga cattactgca
     7681 taaagggaat taatatttaa aattcaaaat gactttatgc tatggcagaa gaaataataa
     7741 tcgaaggtaa gaaagtatct ctttacgaaa taaaaaacaa taaagggatg tcggctgtaa
     7801 ttactaatta tggtgcacgt gttgtgaagc tttgggttcc tgaccggaac ggggagttgg
     7861 ctgatgtggt tttgagtttt gatacactcg aagaaattac aaccaaagaa acctatttca
     7921 attcaacctg cggacgtttt gccaaccgta ttaaggatgg taaattcaca ctcgacggca
     7981 aagaatacag cctcgcgatc aacaacgggc ctaatcacct gcatggtgga ctggttggtt
     8041 tcaatatgcg tgtctgggaa gttaaatcaa ctacagacaa tgcaattgtg ctttattatt
     8101 tttcgcctga tggtgaagaa ggttatccgg gaaatatgga cgtgactgta acttacgagt
     8161 taacggacga caatgaactt aagattcact acgaagctac taccgataag cctacgattg
     8221 caggatttac gaatcattcc tacttcaatc tgaaaggagc cggcgaaggc actgtacgtg
     8281 accatattct ccagttaaat gccgattttc atactgcact cgatgacaac acttgtccta
     8341 caggcgaagt tttgaaagtg gatggaactc cctatgattt ccgtgaaccg acactgattg
     8401 aggaaagaat tgacaatcct gtttatgcat ccaaccgagg attagacgac aactgggtca
     8461 tacgcagaaa ccaaccgcat gaactggcac tggcaggata tatctatgag ccgaaaagcg
     8521 gcaggaaaat ggaagtactc accacaaaac cgggtgttca gatttatgct gccaactggg
     8581 tggaaaaaca aaccggaaaa tacggcaaac aatacgatgc gcaatgtgct gtttgtcttg
     8641 aagctcaagg ctttccggca tcaccggtct atccgcattt cccgtcaccg gtattacgtc
     8701 cgggacaaaa atacgacgaa tggtgtatct acaaattctc cgttgaataa gatatttttc
     8761 ataagtttta atgatggaaa cggttttgtg cttacgggtg taaaaccgtt ttttcctgat
     8821 ttttcaggtc aaaatcacat atttccagaa tacactcacg aatcggtgta aatgtaaatt
     8881 caaaccggga caaaaatttt ttgcaggaat attctttcct gctgatggag atgctggctg
     8941 aagaacggtc aatcaatggg gtgaagccag ctattttccc aataatttca agaacaccgg
     9001 ctaaaggaat cagcaatttt ccgaaaccga taaacgggcg gtatgtatga tatccttcag
     9061 ccatccatcg gagaatatcc ctatgataac aattttctcc agcaatgata aaacgctcac
     9121 cgtttatgtt gctgttcatc agcaatatca ttgccttgca tacatcacgc acatctacat
     9181 aagccgtttt gccgaatgta taaaacggca atcctttttt tgccagtttg aaaagctcgc
     9241 cacttccggt catctttttc gaaatgccaa gaattacacc cggattgaca atcactgcat
     9301 tcaaaccttt ctgaatacct ttttcaattt ctttctcagc cagcattttg ctttctgaat
     9361 aggttgaaac cggcttgtcc ggctcgatgg gtgtattttc atcaatcggt ccgtggctga
     9421 tgtcatcaga aacagccgca atggaactta caaaacacag tttcttcacc ttgttgcaca
     9481 gagctgcatt cactacattt tcagttcctt tggcattggt caggatcatt tcgttgtagt
     9541 ttttgcctaa attgactatg gcggcgcaat ggtaaatata ttccatcccc tcaaacgccc
     9601 ttttcagact attgaaattg agcacatcac ctttcaccca gtcaacccga ctgaggtatt
     9661 ggtcgggtgt attggtgtaa aactcaaaca cggttctcag atcagttgta tttgaattgc
     9721 tgcgtgtcaa agcagaaatt cgcccgttgg attggagcag ataccagatc agatgtcctc
     9781 caaccatacc tgttgctcct gttactaata tcatttttaa gtcaatattt gaatatgttt
     9841 caaaaatacg gaaagaaaaa cgtaattttg caaaaattac aaaataatcg ggagtaaaat
     9901 atgcagaata tttgtgttta ttgtgcttca agcagtaaaa tcgattcaaa gtatttcgaa
     9961 acagcccgtg agctgggagc gatactggca aagaatgatt tgcggttgat ttacggtggc
    10021 ggatcagtgg gagtgatggg tgtcctggcc aatagtgtgc tcgaagacgg cggaaaagta
    10081 accggagtga tccctcagtt tatgtgtgat gccgaatggc agcacgatgg attgaccgaa
    10141 cttgtgatta ctgaaacgat gcacgaacgt aaggaaaaaa tggctttgtt aggtgatgct
    10201 gcagtggcgc tcccgggagg atgtggcacc ctcgaagagt tgctcgagat tattacctgg
    10261 aaacaactcg gcatttacac aaaacccatt gtgatagtta atgtgaacgg ctattacaat
    10321 catttgattg cacaatttca gcaagctgcc gatgaactct tcatgcgtcc acagcatctg
    10381 caaatgtgga cagtagtaga ttctgccgga caaatactgg atgctattgc caatgcgtat
    10441 agctgggatg aaaacctgcg tcagtacgcg aaaatttaga atagacagtt aaaaatcagt
    10501 aatataatgc ccgcatccga tagcatcata aaacttgttg atactacagc cagactaacc
    10561 actgatacgc ttgctgtgtc cgatagcctt tcgaaagcag attcgattgc aggtatcgat
    10621 tcgattaaac tggcagcgtc acttccgcct tccgggatgg aaggtattaa actctcgtca
    10681 ttacctgcaa atgaatcctg ggtttttatt gttctgctgg ttttatttct tttttatgtt
    10741 ggaagtttgc tcaagtctgg aggagaattt ctgcaaaatt tacgatctac tttcaaacat
    10801 aattatacat ggggtacttc cagtgtttcc acacttaatt atcttcagtc caggatattg
    10861 attattcttt gctctgtagg gattttttcg ttacttatac aagaattttt atttgttatg
    10921 cccggaaatt tcgagttaaa aacatttttc ctttcagtgg gggtaacttc cgggttttat
    10981 ttgcttaagc ttttgctgtt ccagatggtg ggaaatgtgt ttttcgataa aaaaacagta
    11041 cttaactatc agaacattta ttttaatttt ctgagtttgt tcggtattgt tcttttaccc
    11101 gtgttaattt tatacactta tcaacctttg tcgtggaagt tacctctcga aattattacg
    11161 ggattacttt tgggatgttt ttatgttttg ctaataataa agttgtgtca ggttttttat
    11221 acaaaaaaat tagcactatt ttatattttt ctgtatcttt gcacgctcga aattctaccc
    11281 cttttggttt tatttcgggt atatgaattg gttatctgaa gttttatttt aaaaaattta
    11341 gcgccttgaa aatcaaaaag atacttgttt cgcagccccg tccgactacc gaaaaatctc
    11401 cttatttcga tataatcgat aagtatagcg taaaaataga ttttcgtccg tttatcaaag
    11461 ttgaacctgt tactgcaagg gaatttagat cacagcatgt taatattctt gatcacacgg
    11521 caattatttt taatgcgcgg catggaattg atcatttttt cagattgtgt gaagaaatga
    11581 ggattactgt tcctgaaacg atgaagtatt tttgtgtatc cgaaactgtt gctctttatc
    11641 tacagaaata tatacaatac aggaaaagaa aaatcttttt cagtcctacc ggtaagtttc
    11701 ctgatttaat tactgtgttg aacaaacatc acgaggaaaa attcctgtat ataacttcag
    11761 atatccgcaa tgaagaaacc atgtcaatac tcgagaactc aaaagtaagc ttttctcagg
    11821 gtattatgta ccgtacagtg agtaataatt tcgctccgga tgaacctttc gattatgata
    11881 tgcttatttt cttcagtccg gccggaatca aatcgctgct tgaaaatttc ccgaatttcg
    11941 aacagggaga aattcaaatc ggatgttttg gagccattac agcgcaggct gtacgcgatt
    12001 ccggactgag acttgatctg gaagttccgt cacccggagt tccttctatg accatggcgc
    12061 ttgataatta tctgaaagaa gccaaaaaga ataacggcaa aaagtaagtg cacagactga
    12121 aaatcagcta aatatatccg gatatcccaa atgtatcgaa attttcgtac ttttgggatg
    12181 tcttttttta ataatctgat gaatacattc cctaaaaaag agcatctttg tggtgagaaa
    12241 atcattacac gacttcacca gcaaggtcgg gcttttatag cttttcctta cagggtggtg
    12301 tatttggtag ctgatgaaaa tgaaacagag cctgtacgcg tgatgatttc tgtccccaaa
    12361 aaacgcttca aacacgcggt ggacagaaac agaataaaaa gacttacccg tgaagtttac
    12421 cggcttaata aacacgaaat tacaaaattc gcactggaac ataacctgaa atttcatttg
    12481 tcttttcagt atgtaagcaa tgaagaggaa agttttgaaa tgcttgaaaa gaaaatgaaa
    12541 cttgcgctga aaaaattaat ccttaatttt tctgaaccga actgcaaaaa tgaaacaact
    12601 ccttaaaata ctcgaattca ttgtgctgat acccatatac ttttaccggt atgctatctc
    12661 gccgctgaag catccctcat gccgatatac tcccacctgt tcggaatatg cgatacaggc
    12721 catcaaaaag catggaatcc ttaaaggcat ctggctagga acccacagga tactaagttg
    12781 caatccctgg ggagggagcg ggtatgatcc tgtacctgaa aaattcagtt tcaaaaaaaa
    12841 atagtatctt tgcgcccgct atttgtgata tgtctttccg aaggaggtat taatcctgag
    12901 acaaaattac aattgcaaca ataaattaat ccacacgtgt gcttgaatac cacgttaaaa
    12961 aaacaagaaa atgaaaaaac aagtttccgt tgcctttatg gccagcgggt tgctattcac
    13021 aacctgcctc atcgtagcaa acatcgtaga acaaaaactg atttcaatcg gaccgattga
    13081 agccactgcc ggtttgctca tttttcccgt ttcctacata gtaaatgata tcatcgccga
    13141 ggtgtggggg tatcgcaaag cccggttgat tatctggtac ggctttctga tgaactttct
    13201 ggctgtagtt atattccgcc tgagtattat tgttccgggt tctgagaact ttactcatca
    13261 ggatgcattt caactggtgc ttggaaacac agaaagaata agtctggcaa gttttattgc
    13321 ttttctgata ggatcatttc tgaatgcaat ggtaatgagc aaaatgaagt tattgcagaa
    13381 aggtagaaat ttttcattac gggctgtggt gtccaccctt gtgggcgaag gagccgattc
    13441 actggttttc tttaccattg catttgccgg agttattccc aacaatgatt tactgatgct
    13501 catactaaca caggctggaa tgaaaactgc ctatgaaata ctcgtattac cactaacaaa
    13561 tgtaattgtt cgctgggtga aacgtcggga gcaggaagat gtgtttgatg atggaatatc
    13621 ttataatccg ttaaaaataa acgaaatata atattatctt gaccttcggg tgctgagtcc
    13681 gttgttgacg gcgtagtatg gattgataat acagacaata accttctcat accagttgaa
    13741 accgtttttt cgcattccca gcgtggcttt gtggaataat tctcccaaca cagtacctac
    13801 aatgggtgtg acaatcagat cctgaatact gggttgctcc atcccggctt cccacacgta
    13861 ttcccatgct gccgattgtg ccagattgta aagcgatgat tcgagaaagg tggatccctg
    13921 tgaacgtatt gtgttgaaat acagtgatcc ctgataagga tgtccaacgt agttggtaaa
    13981 aaacaagtcg tggtctatga ccggaggatt ggtatatgcg ttcttaaatc tttgccttat
    14041 catataccat ttcagcctgt catcatctgt ccacatcgaa aattctttcg ggctcagtac
    14101 cagtgcggtt atcattgtgg cattgacagc agagtttatc aaaacacctc ttaccagctt
    14161 tttcccgatg ggtgcatcct ggtttctgag tttgaattca ggaaagatta cattattttc
    14221 agtatcgcag gtattaatac cagagtatga tttctctgaa agcgtgtcgc ttgcagcctg
    14281 aatcacagaa tccggattat tttgcgcccg ggaaaaagca actgaacata aaatgcaaat
    14341 aatgaataag ctccttttca tggttatgca gtactggaag aataataaat gttatagact
    14401 ttgaatgata taaatttttg ttcgtcaaag atataaaatc aaattcaatt tgcagaaaat
    14461 aatttaaaaa tgtatttact gtatcggata aaatccccgt aaacatccat attcatgcta
    14521 tatgtctgga aaagaacagc ctccggttct ttttttaaac ggaggctggc ccttaaattc
    14581 agtttctatt ctgttgaaac attttttact gaagaatgat cttttttgtg agcaatttgt
    14641 ttcctgaatt gatccggatt acgtaaatgc cttttgaata gttcgataag tcgattgatt
    14701 ttttcctgtc atcggctttt aatgtggaaa aaatgatttc gcctgtagaa ttgaaaattt
    14761 caagtccgtc aattttttcg gaacacgata ccgtaaattt ccctttgttt ggattgggat
    14821 acacattgaa tctttcgctg gataaagact gaacatcggt gccaggacga tagtagtatc
    14881 gaacactgtc gcctgatgaa actgtgatcc catcctgatc atacgatttg tctgattctg
    14941 tcattggata attattcgaa tcgtaagttc ccagcataag atcggacagc acccatgagt
    15001 tgtttaacca ggttttttcg gtatcacgtg taacattccc gtttgcatcg tatgtgtatg
    15061 tgtcttgttt gtaattaacc catgtatttt ctgtccattc ctggtgcaat tcagttgcaa
    15121 catcgtcaga ggcattatag gtataggttg ttttatattg gtttttccat gctccggcat
    15181 cccacacctg cgttgtttgt gttgtttcat tattacgggt atcgaatgtt gatatccact
    15241 gttgcgaatt atcccagtta ccattgttcc agttctgata aagcaagttt gtattgttgt
    15301 cattcgcatc ataggtgtaa ctatgtttcg attcgtttac ccatttgttt gcttcccact
    15361 tttgcgtggt tactgatgtt ataagttcgt gattgttgta agtgttaact aaataactac
    15421 tgttcaccca ctgatttgtt tcccaggttt gctgagttga ggtcaatagt ttattttttg
    15481 catcgtaggt aaaaatttct ttcgaaacat tggcaagcgg accgaacata ccggattggt
    15541 ttacttctgt cagtacattg tgattggcat cataggtaaa agtgcttagt gatatggtcg
    15601 tccagtcggt cataaaccac atctccaatt ttgttgtcgt tgaattttta ttttcatcat
    15661 aggttatggt tgttttcatg ccatttgtcc agttgctgcc gtcccaggtt tctatcgaaa
    15721 gcatggtcat caaatcacta ctgttgtagg tgtaagtaga ttttgaagtg ttttcccatt
    15781 cctgggtgtc attattccat tcctgttcaa ttgaacttat tattcggtct ttattgtcgt
    15841 aggtaatctt gatcgtttta tgatcgttga cccagccctt cgtattaaca tcccagttcc
    15901 agtagcggac actatcgagc aattcgtact gaattgccaa agcctgttgg gctttgatgt
    15961 attcgttgat tttttcggtg gtcagaactc cgggtgtttt ccaggtactt tttttgggaa
    16021 aagatttaac aatttccaat ggacttttca caggtttcct ggctgtacca ggcaacagtt
    16081 gattcgcgaa aatagcatta gctgaaaatg ctaacagtac aaaaagtaaa aattttttca
    16141 taatggtgtt tttttgtgtt atatttagat attgaaaaaa agaccgattg aaaaagtttg
    16201 aaaaacgggg ttttaatcag atgaatttca tcatattgaa ttacataacc ttgttcagca
    16261 gattggcaag ttgaatgcct ccccgataaa gcagttcgtc cagatcgttg ttgaaaacat
    16321 acatgtaatt atacgctctc agattattgt aatcgtactt atatattttg ttacgaacct
    16381 gataaacggc ttcgagtgac tgtggcagcg actgtttttt aaattcatta tttctgccag
    16441 caaatttatc ctgaagaaat tcaacgtatt ccgaatagga ataattgcgt ccttcaatca
    16501 tctgtgtatc ccagagctga tgaagccgta tatcttttcc gaaccatttt atcatgatat
    16561 cgtttccacc acggtctccg gctcgtccca aatgcatcgg ttggtgagca tcggcaatga
    16621 aatgaatcaa aaactttagc gcctcgatat cttttttgtt gtttttcagg ctttggctca
    16681 ggctgtcgat ggcgaaaaac agatgaagcc cttcagtttt tggtttttcg tataaagaaa
    16741 gaatatcggt tggagtcata ccatcacgca ggttctggaa atgccaattg taactgtagg
    16801 cataagcaga atcgcttcga acatcatcgc tccaggtcga ttcgtaaatt aatccgtgtt
    16861 ttcctaacag tttatccact ttcttgcgtg catgacaggt aagattttca taagcaattc
    16921 cggctataat cctatgaccg aaagcatcat aagccatagc aggcaaagtc aggctgattg
    16981 caagaaggaa aagaaaaaat ttttgtttca ttaatttgtc tgttcttatt gaagtgtaaa
    17041 gatatacttt ttttaattaa attgtccgat tatatgttgt catttttttc caaaaaatta
    17101 tcagattaat ttatacttca ttctgtcccc actatttttg agtggtattt tcatccatga
    17161 aagtgtaaaa atgtttgatc tgaataagtg aacaaataat ttttcaaaac taaacacacg
    17221 gattttattt atctttgtaa caaatctcaa accgatacag acgaacatgc tttcaattct
    17281 tgatgagtat cacatctacc tgaaaatgga gaaatcgctt tcggaaaata ccattgaagg
    17341 ttacgaacgt gatttgaaga aattattctc gtatctggcg gatgcacata ttttacccga
    17401 agaggctgaa gacagacatt tgcgcgattt tatcatcgaa acaggtgaaa tcggcatcaa
    17461 tgaacgttcg ctggcaagac ttatttcaag tatcaaatcg ttttataaat tcctgatata
    17521 caatgataaa attacaattg atcccaccga actaattcaa agtcctaaaa tcggattgcg
    17581 gttgcccgat gttcttacgc tgtccgaaat tgaccgcatc attgaagctg ttgacctctc
    17641 aaaacccgaa ggtcaccgca acagagctat tatcgaggtg ctttacggtt cgggactaag
    17701 ggtttcggaa ctcattaatc tgaaaatttc gaatatctat tttaacgatg gatacatgct
    17761 tgtggagggt aaagggaata aacagcgact tgtacccata tcggatgaat cgaagaagca
    17821 gattgatttc tgggtgaatg accgcaacca tctgaacatc aaaaaaggac atgaagatta
    17881 cctgtttctc aaccgtcgcg gcagccgact gacccggatg atgatttttg ccatcgtgaa
    17941 ggaacttgcc gttatagccg gcattcataa aaatgttagt ccgcatactt tccggcattc
    18001 gtttgccaca catctgctcg aaaacggtgc aaatctgaga gcgatccaac agctcctggg
    18061 gcatgagtca ataacaacca ccgaacttta tactcatata gatgttcagt ttttgcggaa
    18121 aacaatactg gagtttcatc cgatgaatca aaaaaagaat tgacatggca ataagagcat
    18181 catttagtcc ggcaattaaa agacgcatcg tttttctgtg tgtgttattt ctatcagcat
    18241 tcatacatct agcagctcag gaaacagtgg ttgtcggaca ggtgattgat aagtacgacc
    18301 aatcgcctat tgcttcggtt gatatttatt tcaaaaactc taatgttgca gttcagagca
    18361 acgatgaagg atatttttta atacgcaaca ccggtccgga aacaacattg atatttactg
    18421 ttatcggtta caagtcttat gaattgaaac tgaaacgggg cgataatgtg ggagtgcaga
    18481 ttgaactgga agagaaaaag aatattcttg atgaagtgtt gatcataccc ggagttaatc
    18541 cggctgttga cctgatgaaa aaagtccgac agaaacgcag gcagaacgat gtgaaggtgt
    18601 tgctttcagc tacagatcaa agtgctgttt tcctgagtaa aaacgatgcg cgctggcaaa
    18661 acaataaact ctttgagcaa ttgaaatccg gaaatctttc tgaaaacgat tctgtgttgc
    18721 tgatgccaat ttatatggtt gaaagtgagt acgatgcgaa agggctggta aagcgtcaga
    18781 agaataaaaa gacctacaat actgacgaaa cgacagaaaa tctggttgat aaactgctcg
    18841 acggaatgga taaccgcatt agtttctacg ataattcagt taatctcctg ggtaagaata
    18901 tgatcagtcc gcttgccagt ataggcagta cattttatcg gtattacctg accgatagca
    18961 ttctcacgcc aaccggtaaa caatatgata ttcattttta ttcgagaaat cccaaaagtc
    19021 tggctttcaa tggaagtatg tatgttgact ctgcgacttt ggcactgaca tatatctcgg
    19081 cttcgcttcc gaggcaggct aatctcaatt ttatacacaa tctcagtttt gtacaaaaat
    19141 tcaaaaagac tgagaattac tggatccctg acaatcagca atcgacctgg tacttgactt
    19201 acgatttgct gaagaacgat gccggagcaa gttccgaact gctcgtcagc cggaacacat
    19261 cctattcaag cggcgaaggt gatattgtgc tgcaaaccga ttcgtttgct ggaagcgctt
    19321 tttccgacaa agaaataaat acccggatga tagccctgca gcaatcaaaa attttcaaag
    19381 ctgcaagcat cattgccgat gcaggcttga ccggttacct gaaagcaggg aagatagatg
    19441 tagggctgct tgcaaccatt ctgcgcctga ccgaagaaga aggcttgcgc atgggactaa
    19501 acatgagga
//
LOCUS       DAOPYB010000039        19467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2927_length_19467_cov_8.445601, whole genome shotgun sequence.
ACCESSION   DAOPYB010000039 DAOPYB010000000
VERSION     DAOPYB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19467
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2927_length_19467_cov_8.445601"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..546)
                     /locus_tag="PKH58_04800"
     CDS             complement(79..546)
                     /locus_tag="PKH58_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX88377.1"
                     /translation="METTEKDRIEKVMLVEKLTATQFAAEIGIKTPTLSHILSGRNNP
                     SLDVLKKILERYRTISSDWLILGVGQMYRVSRQSQTPTLFENQEESDSNSERYAQKKV
                     EKNVDDESTIYKKIPVELPVTAIEPSLITEKSKKIKNIIIYYDDSTFQEFVEK"
     gene            complement(1450..2223)
                     /locus_tag="PKH58_04805"
     CDS             complement(1450..2223)
                     /locus_tag="PKH58_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNX88378.1"
                     /translation="MATETKTDKEITVEEKLKALYELQKVDSKIDEIKILRGDLPLEV
                     QDMEDEIVGLSTRLVNFEKEIAELNTAIKGKKVEIDEANIKIERYKEQQNNVRNNREY
                     DNLSKEIEYQALEIELCEKRIREFNASLENKKIELDNTSVQLSERKLDLEQKKGELEE
                     IVSETKQEEEKLREHAKKIEARIESRLITAFKRIRKNARNGLAVVSVQRDACGGCFNK
                     IPAQRQLDIRSRKKIIVCEYCGRILVDPDLGGQESSETK"
     gene            complement(2227..3324)
                     /locus_tag="PKH58_04810"
     CDS             complement(2227..3324)
                     /locus_tag="PKH58_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNX88379.1"
                     /translation="MTTVKTITGWIEEVAPLALQEDYDNAGLLIGSPDMVVNGVLITV
                     DVTEPVIEEAYNIGCNLIISHHPLIFRGVKRLTGQNETQRCIIKAIKHNIAVYAAHTN
                     MDNVLSGVNGKIAEKIGLKNMKILLPKKQTLLKLVTFVPVNHISSVQNALFEAGAGHI
                     GNYDNCSYSSEGNGTFRANEKANPFVGEIDVTHTEAEIRLEVIMPGYLKEKIIRALLN
                     AHPYEEPAYDIIPLDNALSIVGAGIIGELPGEKDELQFLHQLKSIFQLDTLKHTSLLN
                     KTIRKVAICGGSGSEFLPEAITAGADVFISADFKYHQYFDAENKILIADIGHYESEQF
                     TKDVFYEIITKNIPKFAVRISDVNTNPIKYL"
     gene            complement(3334..3894)
                     /locus_tag="PKH58_04815"
     CDS             complement(3334..3894)
                     /locus_tag="PKH58_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNX88380.1"
                     /translation="MLKEILSITGKPGLYKHVSNGKNMLIVESLIDGKRIPAYTKDKA
                     VSLGDIAIFTENEEIPLGQVLENIKTKENGVKCSVDTKADNEQLRKFMAEILPEYDRD
                     RVYPSDIRKIINWYNLLIEANLVDFLEKKQVEEEVKTEKEIDLKEKTKKEVKPATVAK
                     DVKQPKQQTKVNKPATQRSTNSRKMG"
     gene            complement(4049..4771)
                     /gene="hisA"
                     /locus_tag="PKH58_04820"
     CDS             complement(4049..4771)
                     /gene="hisA"
                     /locus_tag="PKH58_04820"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNX88381.1"
                     /translation="MIEIIPAIDVIEGKCVRLTRGDYNQKKIYNEDPLEVARMFADAG
                     IKRLHLVDLDGAKAQHIVNYKVLEQIVSKTSLTVDFGGGLKSDKDLQIAFECGASMIT
                     GGSIAVKNPDIFIKWLNKYGSDKIILGADVMDEKISVSGWTETTNIELIPFLKIYTDK
                     GITKVICTDISKDGMLEGTAVKLYQKILSELPQMYLIASGGVSCMQDIENLILAKVPA
                     VILGKAIYEGKVQLKDLSGFFK"
     gene            complement(4776..6569)
                     /locus_tag="PKH58_04825"
     CDS             complement(4776..6569)
                     /locus_tag="PKH58_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNX88382.1"
                     /translation="MCGIAMVRLLKPLDYYQEKYGTWQYGLQKMYLLMEKQHNRGQEG
                     AGLATLKLNMPPGEEYIWRDRKEGKNAIQEIFASVNEKLETYKGVAEDAEFAKTNLSF
                     AGELYLGHLRYSTTGRSGIANVHPFLRRHNWKSRTMVLAGNFNLTNVDELFEMLTNQG
                     QHPRDKADTFLMLESLGNKLDNEVQAEYEKIRQRYTNDVRITQKIEDQLDISSFIRKS
                     EKYWDGGYLITGMLGHGDAFVFRDPKGIRTGFYYIDDEIVVVASERAVIQTVMGVNEE
                     KIFELEAGKSMIIKKNGNIQYETIRVASPEQKPCSFERIYFSRGSDLAIYQERKKLGA
                     TLAKPIMEAIDNDLDNSVFSYIPNTAEVAFYGMTQGIRQITGTDPHIEKVLIKDIKLR
                     TFISQNKERNDLAAHVYDVTWNSIRRGEKDNLVVIDDSIVRGTTLKQSILKILNRLEP
                     KKIIIVSSSPQVRYPDYYGIDMAKMGEFCAFRAAIALLKETGRQNLIDEVYRKSKEQE
                     SFHKEMIVNYVTEIYKPFSQEEISDKIAKMLTPEEVNCPVQLVYQTIEGLHLACPNHS
                     GDWYFSGNYPTPGGNRLVNKAFINYYEGKEV"
     gene            complement(6612..8453)
                     /gene="glmS"
                     /locus_tag="PKH58_04830"
     CDS             complement(6612..8453)
                     /gene="glmS"
                     /locus_tag="PKH58_04830"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNX88383.1"
                     /translation="MCGIVGYMGNKQAYPILIKGLHRLEYRGYDSAGIALIDNENLKV
                     YKAKGKVAELEQFAEQKNTEGTIGIAHTRWATHGEPNQKNAHPHYSQSEELAIIHNGI
                     IENYAVLKQGLTEHGFTFKSQTDTEVLVQLIEYMKKTNEVDLPTAVQLALNEVVGAYA
                     IAVIEKSNQDMIVAARKGSPLVIGIGTDEFFIASDATPIVEYTDKVVYIGEEEIVTLR
                     RNEPMKIRTIANIEKTPEIHKLELTLSQLEKGGFPHFMLKEIFEQPLTLADSMRGRVN
                     VEANNITLAGFIDNKDKFLKASRIIITACGTSWHSAQIGMYAIEEFARIPVEVEYSSE
                     FRYRNPVINKDDVVIAISQSGETADTLAAVELAKQQGAFIFGICNVVGSSIPRNTHSG
                     CYTHVGPEIGVASTKAFTAQVTVLTMLALLIGKEKGTLSEQHYLRLINELNLIPEKIS
                     TILKKNDVISEFSKTFTYAQNFIYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMK
                     HGPIALISQEMPVVVIATNSSTYEKVISNIQEIKARKGKIISVVTEGDEIVKALSDFS
                     ISIPETEECLSPLLAVVPLQLLAYHIAVVKGCDVDQPRNLAKSVTVE"
     gene            complement(8467..9687)
                     /locus_tag="PKH58_04835"
     CDS             complement(8467..9687)
                     /locus_tag="PKH58_04835"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNX88384.1"
                     /translation="MALANENYLKVPQIYCFDEIEKKVNAYKLLHPKAEIIRLGAGDV
                     TRPLPAEVIKAMHKAIDELGHENSFRGYSPPQGYDFLITKIQKEYKTLGISLAKETIF
                     INDGAKSAIGNIGHVLGRDNIIAIAEPVYPVYENATIMSGRAGNIIDESKWSNIVYLA
                     CNPENNFIPELPEEKVDVIYICNPNNPTGIAMNRAELKRWVEYAIHHHSIIVYDGAYE
                     AYIQQDDIPRSIYEIKGAKKVAIEVRTFSKTAEFTGLRCGYAVFPHELNVFTKFGESV
                     PLVDLWSRRNSNYTNGVSYIVQRGAEAVFSPKGKQEVNEIINYYITNTQLVRNELIAL
                     GFEVFGQVNSPYIWFKVPENMSSWKYFNELLFQKNIVGTPGVLFGSKGEGYMRFTGFS
                     SRENILKALDRLKN"
     gene            complement(9843..10847)
                     /locus_tag="PKH58_04840"
     CDS             complement(9843..10847)
                     /locus_tag="PKH58_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HNX88385.1"
                     /translation="MEQITYRAFVVEETEGKYFSSIKKQNIDQLPAGDLLIRVHYSSL
                     NYKDALSASGNKGVTRKYPHTPGIDAAGVVVSSGNKAFNPGDKVIVTGYDLGMNTSGG
                     FGEYIRVPAEWAVMLPENLTLKEAMIMGTAGFTAGISVTRLTELVKPSQGKIIVTGAT
                     GGVGSVALSILSKLSYSTVAVSGKLAEYDYLKNLGADEIISRNDFLDMEKKPVLSAKY
                     AGGIDTVGGPYLENILKTLSPLGAVTTCGSVASTDLNMTVFPFILRGISLIGVSAQNY
                     PMHLREFLWNRLAADWKPENLMQIYNEINLDQLAEAIRSILKGQLKGRTIVNMEDSIV
                     "
     gene            complement(10964..11332)
                     /locus_tag="PKH58_04845"
     CDS             complement(10964..11332)
                     /locus_tag="PKH58_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNX88386.1"
                     /translation="MATVNENQLNVLTFGTKIVGKITAESDFRLDGEVEGDIVCKGKV
                     VIGQRGILKGSITCVNAEILGKVIGNFHIAETLSLRATAEISGDVKTKSLVVEPNAVF
                     NGSCSMREVASQQTPSQVNK"
     gene            11624..12496
                     /locus_tag="PKH58_04850"
     CDS             11624..12496
                     /locus_tag="PKH58_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88387.1"
                     /translation="MQIRRLHHHEINYDLWDKCINNAKNSLAYASTWYLDMVSPGWEA
                     LVAGNYEYIMPLPVKKKYVIKYIVQPILTQQLGVFSEETIDSEIVNSFIRAIPYFSYE
                     LNLNERNFTPKAAILPNIIVDLNYPYEYLKSKFSINTKRNIVKAESNQLHIDQSLKPD
                     EFIRLYLETDRNFKIIHADKIRNLIYKGSENNLISICCVRTPENEVVSAVGLLKSGNR
                     IIYLLPFSSVKGKALSAMFFLINELIKEHAGTEKILDFEGSRIEGLKRFYKGFGGVER
                     SYYLIKKCRPKFRK"
     gene            12558..13658
                     /locus_tag="PKH58_04855"
     CDS             12558..13658
                     /locus_tag="PKH58_04855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX88388.1"
                     /translation="MAGLFVRKHAEAVSLYCDVRVLYVHADKKINRFEVEKRTDNSLT
                     EYILYYPAGKNFFFGKATAIINYFIAYHKGIKMLSEENWKPDIIQANIFTRTALVACL
                     MKLKYRIPFVVIEHWTRYFREKTFNNFLHRLITVWVAGKAAAVLPVTKHLQEWMQYHG
                     IKNDNYQVINNVCEDNFFSKATKQDNQKIRILNVTCFNDNHKNISGILRVICELSKKR
                     HDFELILIGDGEDFESMKAYARELKIEAGYIQFTGLLEGEKLIEQYQQCDFTVLFSNF
                     ENIPVVISESLACGKPVISTNVGGINEHITPENGILIPAKDEKALLDSISWMIDHYKQ
                     YDAKKITNDAYEKYSYNEVGKKLLNIYLKIVE"
     gene            complement(13663..14805)
                     /locus_tag="PKH58_04860"
     CDS             complement(13663..14805)
                     /locus_tag="PKH58_04860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX88389.1"
                     /translation="MDKIRKINVFVPGDSSLLNTWSNIPYLLTMTLEKQGYIVTRINI
                     EPNHFFQKFWNNVLFRVISKFFRNTIYWYDRTLICYIETFIRIWFQNRKYNDADLNLF
                     VSYSFYNPFSSKPNVLLSDWDYETMIKEKRLRNPYFFEKWSIKRQKKIIQRADLVISL
                     FSSAAEDMRVRYNKSNIVCLGKNVINVFFDREINENEILERKQISNKLLFIGSIKYFE
                     GAKLLIDSYSRLKPLYPLLELVIIGIGKEYFPALPEGVTCYGYLDKGNKKENERYYDE
                     LINARVIINATPVWAGYSSTIEGMYFYTPVIVSPYSDFVNEFGKELDFGFYCQEFDVN
                     SLKETIELLFQRNDYQQLCINAREKVKEYTWENYVTKLMTEINNLQ"
     gene            complement(14841..15860)
                     /locus_tag="PKH58_04865"
     CDS             complement(14841..15860)
                     /locus_tag="PKH58_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88390.1"
                     /translation="MIKVSSDSKIYVICPADRETGGTELAHQLVDCLIHKGIDAYIVY
                     VDDEKYVDAEIPVSFGKYLVHVAPEPVDDEKNVVVCPEAIFSFALSLKKIQIIFWWMS
                     VDNFFLDARMFSQLNFFGIYTTLRYVYGRYIRHQPLFGKMTISKLKSVKNRHLHVFQS
                     EYARQFLLSVGINDPMPLSDYIDTDFINNSLTDLHKKENIILYNPRKGFEFTKKIIDQ
                     LPDYKFVALQDMNKSELHGFLKKAKLYIDFGNHPGKDRLPREAAVNHCCVITGTKGSA
                     KYIEDVAIPDVYKLEKNELATIKHLIIDVLENYEQHISAFGDYRQKILNEKSVFESEV
                     ERIFF"
     gene            complement(15857..16966)
                     /gene="glf"
                     /locus_tag="PKH58_04870"
     CDS             complement(15857..16966)
                     /gene="glf"
                     /locus_tag="PKH58_04870"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HNX88391.1"
                     /translation="MRYLIVGAGFSGAVVAEQLSRDTDNEIIVMDERSHVGGNCYTER
                     DAETDVMIHRYGPHIFHTDDKEVWEYICRFCDMKPFINRVKTVSKGKVYSIPVNLHTI
                     NQFAGKAMNPQEARNYIEHKADKSIENPRNFEEQALKFIGRELYEAFFYGYTKKQWGC
                     EPDMLPASILKRLPVRFNYDDNYYDDQFQGIPAEGYTEIVNRMLDRTNIKVQLNAKFS
                     PKMDLSGYNHVFYTGPIDEFFAYKYGRLSYRTVSFEKNVCEGDFQGNAVINYADEEVP
                     YTRIHEHKHFTQWENHRKTVYFKEFSKETMPGDMPYYPKRLSEDLVKLELYQKEAQDL
                     QNVTFLGRLGTYRYMDMKDVIREAIDCAKNFSRIL"
     gene            complement(16963..18285)
                     /locus_tag="PKH58_04875"
     CDS             complement(16963..18285)
                     /locus_tag="PKH58_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNX88392.1"
                     /translation="MQKTQLLKNIFSLGTVQLVNYVFPLITVPYVSRIIGPESYGIIN
                     YATAFIAYFTLLIGYGFDLTATRKIARNPNDNDYVSTIFSQIVNARILLFLISALLFL
                     ICILVIQPLQNNIYVTLILFLSTVSVVLVPQYIYQGKQHLSIFAFLNFLKGLISTILV
                     FVFIKTRNDYVILPAINVTVALLGSVFLLLYACRKFNLTFHWVKIKSSIRLLYEERVI
                     FISTVVISLYTTTNTVVLGLFVSAAEVGYFTVSLSLLNVINSVISIPVSSAIFPFIGN
                     SFAKNRETGLDVIRKVLPMISYLTFFAGIVLFIFAPLIINLFYGNQFHDAVLSFRIMA
                     FTPFIISLSNVFGIQTMLNLNMDKTFFKATATASIIGLLLNILMSKFYGFVGTAWNTI
                     IIESFVTVYMYYLIRKNNLSIFEKKYFNPKFAVSVARNIFTKNNSQDK"
     gene            complement(18639..>19467)
                     /locus_tag="PKH58_04880"
     CDS             complement(18639..>19467)
                     /locus_tag="PKH58_04880"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNX88393.1"
                     /translation="KSNDLKNWQFMKPVFDVSPEWAKKEVPKFDGNIWAPDISFHNGQ
                     YFLYYSISSFASNRSCIGVATNVTLNPSDPRYKWVDHGKVIESVPGRDNWNAIDPNLV
                     FDENGKPWLMFGSFWGGLKMVQLTDDLLSIAKPEAWYTVASRQRDKMLDETDPGDASI
                     EAPFVFRKGKYYYLFASFDLCCRGEKSNYNVRVGRSEKLAGPYIDKEGKSMLHGGGTL
                     IAGSDEQFYGKGHNSVYTFGGADYMFLHGYEKTYRGRPTLLIYKLNWDNEGWPYISD"
BASE COUNT         5873 a         3786 c         3257 g         6551 t
ORIGIN      
        1 cggattttca ataacatata ctcgcttgaa ttatttatac cggacaccaa taattcagga
       61 tattaataaa tatagaagtt atttttctac aaattcctga aaagtactat cgtcataata
      121 aataataata ttctttattt tttttgactt ttcggtaatc agagaaggtt caattgctgt
      181 tacgggtaat tcaacaggta tttttttata gattgtgctt tcatcatcta catttttttc
      241 gacttttttt tgtgcgtatc tttctgaatt actgtcgctt tcttcctgat tttcgaataa
      301 agttggcgtc tgagattgtc ttgaaacgcg atacatttga ccaacgccca aaataagcca
      361 atctgaactg attgttcgat atctttccaa aatttttttc aagacatcca gacttggatt
      421 atttcgtccg ctaagaatat gtgatagtgt aggagtttta attcctattt cagcagcaaa
      481 ttgtgttgct gtcaactttt caaccagcat cactttttct attctatctt tctcagttgt
      541 ttccattatg tgtaaattat tgaatttaca aaaatatatt ttacaacaaa tgtatgtaaa
      601 ataatttaca tataaaaatg atgcacattt gtaaatcaag ataatcgtaa tttacatatg
      661 taatcttatg atatttgtgg aataacaata gtaaaatgat tatattacct aaatactagc
      721 aaattatagg gtatatttaa acttttatat aatgtaaact ttttatttag tttaaatgat
      781 ttatatattt gattaataga tacataatgt atattaaact ccaatttaat caaacaaatc
      841 ctgatatttt acaaatgtat tgaataagtt aaattaattt gttatattta tattgtattt
      901 cgctggtttt catgatttta tattgtatgt tatatctgtt ttacagatag gacttctgct
      961 tattttataa atgtaaatta ttttcatttg taattatact tgtttatatt ttggaaatat
     1021 atttataaaa ttatccattt acaaatgtat ttttacattt gtaaatggat aaaatattat
     1081 tcttatgata aatttctatc aaatatggat atcgatagac tgagtaatta ataatcctga
     1141 ttaattacat ctcatatttt tataaatacg gtagaataat aattgtgaat agttaatttt
     1201 aataaaaatt caatgaaaaa tagtatggag acttatattt ttttttctga gcataagagc
     1261 atacagaaat atggcatgta agctactgaa atacaagatt atacataaaa taatagcctc
     1321 tgagatttgc ttatttgaat tttacatgta acaaggataa gttcattcaa attataagca
     1381 aaaaaaatcc gaacaaatag atttgttcgg attttatatt tttaaatatt ctaaggaatg
     1441 caacaataat tattttgttt cggaactttc ctgccctccc aaatcggggt caaccagaat
     1501 gcgaccgcaa tattcacaaa caatgatttt tttacgtgag cgaatatcaa gttgccgttg
     1561 tgcaggaatt ttattgaaac aacctccgca tgcgtcacgc tgcacagata caacagccaa
     1621 accatttctg gcatttttac gaatacgctt gaaagcagta attaaacgtg attctattct
     1681 ggcctcaatt ttctttgcat gttcacggag tttttcttct tcctgcttgg tttcggatac
     1741 aatttcttca agttcgcctt ttttctgttc caggtcaagt ttgcgttctg aaagttgtac
     1801 tgaggtattg tctaattcaa tctttttatt ttcaaggcta gcattgaatt cccgaattct
     1861 tttttcacag agttcaattt ctaatgcctg atattctatt tcttttgaaa gattatcata
     1921 ttcacgatta ttccgaacat tattctgttg ttccttgtag cgttcaattt ttatattagc
     1981 ttcatcaatc tcaacttttt tacctttaat ggcagtattc agttcggcta tttctttttc
     2041 aaaatttacc aaacgtgtgc ttaaaccaac gatttcatct tccatatcct gaacttcaag
     2101 gggaagatca ccacgcaaaa ttttgatttc atcaattttt gaatcaacct tttgaagttc
     2161 gtaaagagct tttaattttt cttcaacggt gatttcttta tcagttttgg tttcggtagc
     2221 catatattat aaatatttta ttggatttgt attaacatct gaaattcgga ctgcaaattt
     2281 aggaatattt tttgtaatta tctcataaaa aacatctttt gtgaactgtt cactctcata
     2341 gtgtcctata tcggcaatta aaatcttgtt ttcagcatcg aaatactgat gatatttgaa
     2401 atcagcagaa ataaaaacat cggcaccggc agtaatagct tccggcagaa actcactacc
     2461 cgatccgcca caaatagcta cttttcttat tgtcttattc aataatgaag tatgtttgag
     2521 agtatcgagt tgaaaaatac tcttcagctg gtgtaagaat tgcagttcat ctttctctcc
     2581 tggcaattca ccgattatac cagcaccgac aatactcaag gcattatcaa gtggaataat
     2641 atcgtaagct ggttcttcgt aaggatgtgc attgagtaat gctctgatta tcttttcctt
     2701 cagatatcca ggcatgatga cttcgagccg gatttctgct tctgtatgag tgacatctat
     2761 ctcacccaca aaaggatttg ctttctcgtt agcccgaaaa gttccattac cttcggacga
     2821 ataactgcaa ttatcataat tgccaatatg accagcaccg gcctcaaaca aggcattttg
     2881 aacagaactt atatgattaa ccggtacaaa agtaacgagt ttgagaagtg tctgtttttt
     2941 gggtagaaga attttcatat ttttaaggcc aattttttca gcaatttttc cattgactcc
     3001 actcaataca ttatccatgt ttgtatgggc agcgtaaaca gcaatattgt gtttaatggc
     3061 tttaattata caccgttgtg tttcattttg acctgtgagt cgcttcactc ctctgaaaat
     3121 caacggatga tgtgatataa tcagattaca tccaatatta tatgcttctt caatcactgg
     3181 ttccgttaca tctacagtaa ttaaaacacc gttcacgacc atatcgggac ttccaatcag
     3241 cagtcctgca ttgtcataat cttcctgaag agctagcgga gctacttctt caatccatcc
     3301 ggttatagtt tttactgtag tcattacaag tgactatccc attttgcggc tattagttga
     3361 ccgctgagta gcaggtttgt ttacctttgt ctgttgtttt ggctgtttta cgtccttagc
     3421 aacggttgca ggtttaactt ccttttttgt tttttctttt aaatcgattt ctttttctgt
     3481 ttttacttct tcttcaactt gttttttttc caaaaaatca accagatttg cttcgattaa
     3541 aagattatac cagttgatta ttttgcgaat atcgcttgga tacactctgt cccgatcata
     3601 ttcgggtaaa atttcagcca tgaattttct gagctgttcg ttatctgctt tcgtatcgac
     3661 agaacacttt actccgtttt ctttggtttt aatattttcc aagacttgtc caagcgggat
     3721 ttcttcattt tccgtaaaaa ttgcaatatc acccaatgaa acagctttgt ccttggtata
     3781 agcagggatt cgtttaccat caatcaacga ctcaacgatg agcatatttt taccattcga
     3841 tacatgcttg tacaaacctg gctttcctgt gatagataaa atttctttca acataacaat
     3901 tcagtattaa tcagactttt tcagtcatcc tgtaaataat atttttatag ttaatttcgt
     3961 caaagacaaa aaatgatctg acattttact cttcagatca attttaatac aaatatagtt
     4021 atttattttg cattttttgt tcctccgatt acttaaaaaa accggataga tctttcaatt
     4081 gcactttacc ttcataaatt gctttaccaa gaattacagc cggaactttt gcaagtatca
     4141 gattttcaat atcctgcata caactaactc caccacttgc aataagatac atctgaggta
     4201 attctgacaa gattttttga tataatttta cagcagtacc ttcaagcatt ccgtctttac
     4261 tgatatctgt acagattact tttgtgattc ctttatctgt gtaaattttt aaaaagggaa
     4321 ttaattcgat attggttgtt tcggtccagc cagatactga aattttttca tccataacat
     4381 ccgcgccaag aattatctta tcagaaccgt atttattcaa ccatttgatg aatatatccg
     4441 gattttttac agctatactt cctcctgtaa tcatggaggc accacattca aaagcgattt
     4501 gcaaatcttt atctgatttt aatccacctc caaaatcaac ggttaaactt gttttcgata
     4561 ctatttgctc aagtacttta taattcacga tgtgttgggc ttttgcaccg tccaaatcca
     4621 ccaaatgcaa ccgtttaata ccagcatcgg caaacatacg ggctacttca agcggatctt
     4681 cgttatatat ttttttctgg ttatagtctc cacgggtaag ccgtacgcat ttcccttcga
     4741 ttacatctat tgccggaata atttctatca tcgttttaaa cttcttttcc ttcgtaataa
     4801 ttaataaatg ctttatttac caatctgttc cctccgggtg tgggatagtt acccgaaaaa
     4861 taccagtctc cggaatgatt tgggcatgct aaatgcaaac cttcgatagt ctgatatacc
     4921 agttgaaccg gacaatttac ctcctccgga gtaagcattt ttgcaatttt atcggatatt
     4981 tcctcttgag aaaatggttt gtatatttca gttacataat tgactatcat ttctttatga
     5041 aagctctcct gttcttttga ttttctgtaa acctcatcaa taagattttg tcttccggtt
     5101 tctttcaata aagcgattgc tgctctaaat gcgcaaaatt cacccatttt tgccatgtca
     5161 atcccataat aatcgggata acgcacttgt ggtgatgaag aaacgattat aatttttttc
     5221 ggttcaagtc tgtttaatat tttcagaata ctttgtttaa gagtcgtacc ccgtacaatt
     5281 gagtcatcta ttaccaccag attgtctttc tcacctctgc gaatagaatt ccaggtcaca
     5341 tcgtatacgt gcgcagccaa atcattacgt tctttatttt gcgaaataaa agttcttaat
     5401 ttaatatctt taataagcac cttttcaata tgaggatctg ttccggtaat ctgacgtata
     5461 ccctgggtca tgccgtaaaa agctacctcg gctgtatttg ggatatatga aaagactgaa
     5521 ttatccagat cattatcaat cgcctccatg ataggtttag caagtgtagc acccagtttc
     5581 tttcgttcct gataaatcgc cagatcacta cctcgtgaga aataaatacg ttcgaacgag
     5641 catggttttt gttcggggga agcaacacga attgtttcgt actggatatt tccatttttc
     5701 ttgataatca ttgatttgcc agcttcaagt tcgaagattt tttcttcatt cacccccatc
     5761 acagtttgaa taactgctct ttctgaagca acaactacaa tttcgtcatc aatataataa
     5821 aagccggtac gaatcccttt cgggtctctg aaaacaaagg catctccatg tcccagcata
     5881 ccggtaatta aatatccgcc atcccaatat ttttctgatt tacgtatgaa tgaagaaata
     5941 tcgagttgat cttctatttt ttgcgtgatc cttacatcgt tagtataacg ctgtctgatt
     6001 ttttcatatt ctgcctgcac ttcgttatcc aacttatttc ccagtgattc gagcatcaaa
     6061 aaggtatcag ctttatcgcg gggatgctgt ccctggtttg taagcatttc aaaaagttca
     6121 tcgacgttgg taagattgaa attaccggca agaaccatag tccgggattt ccagttatgt
     6181 cgtctcagaa acggatgtac attagcaata ccgcttcttc ctgtcgtgct gtatcttaag
     6241 tgaccaagat aaagttcacc tgcgaatgac aagtttgttt ttgcaaattc agcatcttcg
     6301 gcaactcctt tgtaagtttc cagtttttca tttacactcg caaaaatttc ctgaattgca
     6361 ttttttccct cctttctatc cctccagata tattcttcgc ccggtggcat attcagtttc
     6421 aaagtagcta atcctgcgcc ttcctgtccc cggttatgtt gtttttccat tagcagatac
     6481 attttctgta atccatactg ccatgtaccg tatttttcct gataataatc cagcggcttt
     6541 agaagcctga ccattgctat tccacacata ttattcttct tgtaattttt ttatagttat
     6601 cttttataga tttattcaac agttactgac tttgccagat ttcttggctg atcaacatca
     6661 catcctttca ctacggcgat atggtaagca agcaactgta aaggcacgac agcaagtaat
     6721 ggcgataaac attcttcggt ttcgggtatt gaaatagaaa aatctgaaag agcctttact
     6781 atttcgtcac cttcagtaac gacagagata attttgcctt ttcgagcctt tatttcctgg
     6841 atattactga ttactttttc gtatgtcgaa gaatttgttg caattacaac caccggcatt
     6901 tcctgactta tcagagctat aggtccgtgt ttcatttctg ctgcagggta tccttcagca
     6961 tggatatagg aaatttcttt cagttttaaa gcgccttcca aagccacggg aaagttatat
     7021 ccacgtccta aataaataaa attctgagca taggtaaatg ttttagaaaa ctcggatata
     7081 acatcatttt tcttcagaat cgtagaaatt ttctctggaa tcaaatttag ttcatttatc
     7141 agtctgagat aatgctgttc tgataaggtt cctttctctt tgcctatgag taatgccagc
     7201 atagtgagaa cggtaacctg agcggtaaat gctttggtag aagcaactcc tatttcaggc
     7261 ccaacgtgag tgtagcaacc tgaatgagta tttcggggta tagatgaacc cactacatta
     7321 caaattccaa agataaatgc tccctgttgt tttgctagtt ctactgcagc gagtgtatcg
     7381 gcagtttcac ccgattgtga aattgcaata acaacatcat ccttattaat gaccggattt
     7441 ctgtatctaa attcggatga atattccacc tctacaggaa ttcgtgcaaa ttcttcaatg
     7501 gcatacatcc caatctgagc cgaatgccag gatgtaccac atgcagttat aataattctg
     7561 cttgctttta gaaatttatc tttattatca ataaatccgg cgagagtaat gttatttgct
     7621 tccacattga ctctccctct catgctgtca gctaaagtca ggggttgttc aaaaatctct
     7681 tttaacataa aatgcgggaa accacctttt tccaactgac ttaaggtcag ttccagttta
     7741 tggatttcgg gagttttctc aatattagca atagttcgga ttttcatcgg ctcattgcga
     7801 cgcagagtaa ctatttcttc ttcgccaata tatactactt tatcggtgta ttcaacaatt
     7861 ggagttgcat cagaagcaat aaaaaattca tcagtgccaa ttccgataac cagcggacta
     7921 cccttgcgtg cagctacaat catatcctgg ttagattttt cgatgactgc aattgcatat
     7981 gcaccgacta cttcgttgag tgcaagctga acagccgtag gtagatccac ctcgttggtt
     8041 tttttcatat attcaatgag ctgaacaaga acttctgtat cggtttgact tttaaatgta
     8101 aatccatgtt cagtgagtcc ctgtttaaga actgcatagt tttcgataat tccgttatga
     8161 ataattgcaa gttcttcgga ttgcgaataa tgaggatgtg catttttctg attaggttca
     8221 ccgtgtgtag cccatcttgt atgagcgata ccgatggttc cttcggtgtt tttttgttcg
     8281 gcaaattgct ccagttctgc tactttacct tttgctttgt aaactttcag attttcatta
     8341 tcaataagtg caatacccgc actgtcgtag cctctgtatt caagtctgtg taaaccctta
     8401 atcaatatcg gataggcttg tttattgccc atataaccca caattccaca cataattttt
     8461 atttttttag ttctttaatc tgtctaatgc tttaagtata ttctcacgac tactaaatcc
     8521 tgtgaatctc atatatcctt caccttttga tccgaaaaga acgcctggtg ttcccacaat
     8581 atttttttgg aacagcaatt cattaaaata tttccaggaa gacatatttt ccggtacctt
     8641 gaaccatata taaggcgaat tgacctgtcc aaatacctca aacccgagtg caatcaactc
     8701 atttcgtacc aattgcgtgt ttgttatata atagttgata atttcgttta cttcctgttt
     8761 tccctttggt gagaaaacag cttcggctcc acgctgaacg atatatgaaa cgccgttggt
     8821 atagtttgaa tttcttcgac tccagaggtc aaccaaagga acactttctc caaactttgt
     8881 aaaaacattt aattcatgcg gaaatacagc atatccgcat cgaaggccgg taaactcagc
     8941 tgtttttgag aatgttcgca cctctattgc tacttttttt gctcctttaa tttcgtaaat
     9001 gctccgcggt atatcgtctt gctgaatgta cgcttcatac gctccgtcgt acacaataat
     9061 actatgatga tgaatagcat attccaccca ccttttcagt tcagcgcgat tcatggctat
     9121 accggtagga ttatttggat tgcagatata aatcacgtcc accttttcct caggtaattc
     9181 gggtataaaa ttattttcgg gattacaagc cagataaact atattgctcc atttgctttc
     9241 gtcgataata ttacctgccc tgccgctcat aatggttgca ttttcatata caggatatac
     9301 aggttcggca attgcaatga tattatcacg gccaagtaca tgtccgatat taccaatagc
     9361 cgatttagca ccgtcattga taaaaatggt ttccttagcc agagaaatgc caagggtttt
     9421 atattctttt tgaatttttg tgataagaaa atcgtaaccc tgaggagggc tgtaacctct
     9481 gaacgagttt tcgtgaccaa gctcgtcgat agctttgtgc atagctttga tcacttcggc
     9541 gggtagaggt cttgttacat cacctgcacc caaccggatt atttcagctt tggggtgaag
     9601 cagcttatag gcgttaactt ttttttctat ctcatcgaag caatagattt gaggtacttt
     9661 cagataattc tcgtttgcaa gagccattga gagataatta attataagcc tttttcttct
     9721 aaaaaaagaa agtgcaaaat tgcgattttt ttttcgatta ttaaagaaat gtgcagataa
     9781 atatggtgca gattttctct tttgtttctt aaatgtagaa aagtttattc attatgcaga
     9841 aatcagacaa tgctatcctc catattgaca atagttctcc ctttaagttg tccttttaaa
     9901 attgatcgga tggcttcggc aagttgatcg agatttattt cattgtaaat ctgcatcaga
     9961 ttttccggtt tccagtcggc agccaacctg ttccagagaa attctcttaa atgcatggga
    10021 tagttttgtg ccgaaacgcc aatcaatgaa attcccctta aaataaatgg aaaaacagtc
    10081 atattcaaat cggtagaagc cacacttccg caagtagtta cagcgcccaa cggagaaagc
    10141 gttttcagaa tattttcgag atagggacca cctacggtgt caatgccacc tgcatatttt
    10201 gctgacaata caggcttctt ctccatatcc agaaagtcat tccgcgaaat gatttcatcg
    10261 gcacccagat tcttcaaata atcatattcc gcgagtttgc ctgatacagc caccgtactg
    10321 taactcagtt ttgataaaat actcagagca actgatccga caccacccgt agctccggtt
    10381 actataattt ttccctgcga aggttttaca agctccgtaa gtctggttac cgaaatccct
    10441 gcggtaaatc ctgctgtacc cattatcata gcttctttca acgtcagatt ttcgggcagc
    10501 attacggccc attcagccgg tacgcggata tattcgccaa agccaccgga ggtgttcatg
    10561 cccaggtcat agccggtgac aattacttta tcacccgggt tgaacgcttt gttgccggat
    10621 gaaactacaa cgccggcagc gtcaatgccc ggtgtatgag gatattttcg ggttacacct
    10681 ttattgcctg acgctgaaag agcgtcttta taattcagcg atgagtaatg aaccctgatc
    10741 agcagatcgc cggcaggcag ctggtcgatg ttctgttttt ttattgacga aaaatatttc
    10801 ccttcggttt cttcgactac aaaagcacga tatgttattt gttccatatt gttatcgggg
    10861 attgaatgtg agaatttaaa ctgaacaaaa aaattcaatt taaataatct gagagttaac
    10921 tgattgtgac tgaactctga ctttttataa aaaaaaagga aaattattta ttcacctggg
    10981 agggtgtttg ctgacttgca acttcgcgca ttgaacacga accattaaag acagcattgg
    11041 gttctacaac caatgattta gttttaacat ccccgcttat ttcggcagta gcacgaagcg
    11101 acaaggtttc agcgatatgg aaattaccaa tcactttgcc caggatttca gcatttacgc
    11161 aagtgataga tcctttcagg atgcctcttt gtccgataac gactttgcct ttacatacaa
    11221 tgtcaccttc gacttcgccg tccaaccgaa aatcagattc cgcagtaatt tttcctacaa
    11281 ttttagttcc gaaagttagt acgtttagct gattttcatt tactgttgcc atatctcttg
    11341 ccatatgtaa ttgataatta aaccattatg attttacaaa aaatctttta ataatgaatt
    11401 caaaaataga tattaaagat gaaattacaa aattttttta taactaattc tttctgaagt
    11461 gcgaacaata tatctacaag tacaaataca gccatcattc gcaatttctt tgaactttaa
    11521 aattcattat tcgaccgtaa tcgcatcatt atagaaattt atcgacgtaa gcaggattat
    11581 tttattcaga aaagcattaa ctttgcttca catactacaa agaatgcaaa tcagacgact
    11641 tcatcatcac gaaatcaatt acgacctttg ggataaatgt ataaacaacg ccaagaacag
    11701 ccttgcttat gcttcaacct ggtatctgga tatggtatcg cccggatggg aagcgttggt
    11761 tgcgggtaat tatgaataca ttatgccttt gcccgtaaaa aaaaagtatg ttataaaata
    11821 cattgtacaa ccgatattga ctcagcagtt gggcgtattc tccgaagaaa cgatcgacag
    11881 tgaaattgtc aacagtttta tacgtgcgat tccatatttc agttatgaac taaatcttaa
    11941 tgaaagaaat ttcactccta aagctgcgat actaccgaat ataattgtcg atctgaatta
    12001 tccatatgaa tatttaaaat caaagttttc aattaataca aaaagaaata tcgtcaaagc
    12061 ggaaagcaat cagttacaca ttgaccagag tttaaaaccc gatgaattta tcagattata
    12121 cctcgaaaca gatagaaact tcaagatcat tcatgctgat aaaatcagga atttgattta
    12181 caaagggtca gaaaacaact tgatttccat ctgttgtgtc agaactcctg aaaatgaagt
    12241 agtttcggct gtaggtttac taaaatccgg taaccggatt atttacctcc ttcccttttc
    12301 aagtgtcaaa gggaaagctc tgtcggctat gttttttttg ataaatgaac ttattaaaga
    12361 acatgcaggt actgaaaaaa tacttgattt cgaaggttct cggattgaag gtctaaaacg
    12421 tttttacaag ggctttggtg gtgtggaacg ctcctactac ctgataaaaa aatgcagacc
    12481 aaagtttcgc aaataacata aataatccaa tgagagtact ttttatatca caatggtatc
    12541 ctaacagata cgacacaatg gcgggcctat ttgttcgaaa gcatgccgaa gcagtgtcat
    12601 tgtattgtga tgtacgtgta ctctatgttc atgccgacaa aaaaatcaac agattcgaag
    12661 ttgagaaaag aaccgataac tcattaacgg aatacatcct gtattatcca gccggaaaaa
    12721 atttcttttt tggcaaagca actgccatca tcaattattt tattgcctac cataagggaa
    12781 taaaaatgct cagtgaggaa aactggaaac ccgacatcat tcaggcgaat atttttacac
    12841 gtacagcttt ggttgcctgt ctgatgaaac taaaatatcg aataccgttt gttgttattg
    12901 aacattggac ccggtatttc agggaaaaaa cgttcaataa ttttcttcac aggcttataa
    12961 ccgtttgggt tgccgggaaa gcagctgcag tactgccggt aacgaaacac ttacaggaat
    13021 ggatgcaata tcacggaata aagaacgaca actatcaggt aatcaacaat gtctgtgaag
    13081 ataatttttt cagtaaagct acaaaacaag ataatcagaa aataagaata ctgaacgtaa
    13141 cctgctttaa cgacaaccat aagaatatca gcgggatact aagagttatc tgcgagttga
    13201 gtaagaaaag acatgatttt gaactgatac ttatcggtga tggcgaagac tttgaaagta
    13261 tgaaagcgta tgcccgggaa ttgaagatag aagctggtta tattcagttt acaggtttgc
    13321 tcgaaggtga aaaactgata gaacaatatc agcaatgtga ttttaccgtt cttttttcaa
    13381 acttcgaaaa tattccagtt gtaatttccg aaagtcttgc ctgcggaaaa ccggtaattt
    13441 cgactaatgt aggtggaatt aatgagcaca tcacaccgga aaacggaata cttattcctg
    13501 caaaagatga gaaagcactt cttgactcga taagctggat gatagatcat tataagcaat
    13561 atgatgctaa aaaaatcacg aatgatgcat atgaaaagta tagctacaat gaagttggaa
    13621 agaaattact gaatatctat ttgaaaatag tcgaataaaa tattattgca agttattaat
    13681 ttcggtcatc aattttgtaa cataattttc ccacgtatat tcttttactt tttccctggc
    13741 atttatacag agttgttgat aatcatttcg ttgaaataat agttctatag tctctttcaa
    13801 actatttaca tcaaactcct gacaataaaa cccaaaatcc aattcctttc cgaattcatt
    13861 cacaaagtcg ctgtatggcg aaactattac aggtgtataa aaatacatcc cttcaatagt
    13921 tgaagaataa cctgcccaaa ccggtgtggc atttattatc accctggcat ttatcagttc
    13981 atcgtaatat ctttcattct ctttcttatt acctttatcg agataaccat aacatgttac
    14041 accttcgggt aatgctggaa agtattcttt tcctattccg ataattacga gttctaataa
    14101 tggatataat ggttttaatc tactataaga atcaattaga agtttagctc cttcaaagta
    14161 cttaattgaa ccaataaaaa gtaatttatt cgaaatctgc ttcctttcca gtatctcatt
    14221 ttcattaatt tctctatcaa agaaaacgtt tatcacattt ttgccgagac aaactatgtt
    14281 tgatttatta tatcgaactc tcatatcctc tgcagcgctt gagaataacg aaattaccaa
    14341 atctgctctt tggatgattt ttttttgtct tttaattgac catttttcaa aaaaataagg
    14401 atttcttagt cgtttttctt ttatcattgt ttcatagtcc caatcactca ggagcacatt
    14461 gggctttgat gaaaaaggat tatagaaact atatgaaaca aagagattta aatcagcatc
    14521 attatacttt ctgttctgaa accaaattct tataaatgtt tcaatgtagc atattaatgt
    14581 acgatcgtac caatagattg tatttctgaa aaatttagag attaccctga ataaaacatt
    14641 gttccagaat ttttgaaaaa aatgatttgg ctcaatattt attcgagtaa caatgtatcc
    14701 ttgtttctct aaggtcatcg ttaataaata agggatattc gaccaggtgt tcaacaatga
    14761 agaatcccca ggtacaaata catttatctt tcttatttta tccattttca acttccaaaa
    14821 ctggatttta tctatttttt ctaaaaaaat attctttcca cctcactttc aaacacactt
    14881 ttttcattca atatcttttg acgataatca ccgaatgcac ttatatgttg ttcgtaattt
    14941 tcaagcacgt ctataatcag atgtttgatt gtagcgagtt catttttctc cagtttatat
    15001 acatccggta tagccacatc ttcaatgtat ttggccgaac cttttgtgcc tgtaattaca
    15061 caacaatggt ttactgcggc ttcacgtggc aatctgtcct tgccgggatg attcccaaaa
    15121 tcgatataca gttttgcctt tttcagaaat ccgtgcaatt cgctcttatt catgtcttgc
    15181 aaagcaacga atttgtagtc gggaagctga tcaattattt ttttcgtaaa ttcaaatcct
    15241 tttcgcggat tatacaggat gatattttct ttcttatgca gatcggttaa cgaattgttg
    15301 ataaaatcgg tatcgatata atcggaaagt ggcattggat cattaatacc gacagatagt
    15361 aaaaattgcc tggcgtattc tgactgaaaa acatgcaaat gcctgttttt cacacttttc
    15421 aatttactga tggtcatttt gccgaataat ggctgatgcc tgatgtatcg cccgtaaaca
    15481 taccggagag tggtataaat tccgaaaaaa tttagttggc tgaacattct ggcgtccaga
    15541 aaaaaattat caaccgacat ccaccagaat ataatctgga ttttcttcag agataaggca
    15601 aatgaaaata tggcttctgg acataccaca acgttttttt catcatcaac cggctccggg
    15661 gcaacatgaa ctaagtattt tccaaaactt acgggaattt cggcatccac atatttttca
    15721 tcatccacat acacgatata tgcatcaata cctttgtgta tcaaacaatc caccagttgg
    15781 tgcgcaagtt cggtacctcc ggtttcccta tcggccggac aaatgacata aattttacta
    15841 tcactgctta cttttatcat aaaattctgc taaaattctt cgcacagtca atggcctcac
    15901 gtattacgtc tttcatatcc atataacgat acgttcccaa acgccctagg aaagttacgt
    15961 tttgcaaatc ctgagcctct ttttggtaaa gttccagttt aaccagatcc tcactgagac
    16021 gttttggata ataaggcata tcgcctggca ttgtttcttt gctgaactcc ttaaaataca
    16081 cggttttcct gtggttttcc cactgagtaa agtgcttatg ttcgtgaatt cgcgtataag
    16141 gaacttcctc gtcggcataa tttatcacag cattgccctg aaaatcgccc tcacagacat
    16201 tcttttcaaa acttactgtt cgataagaca acctgccata tttataagcg aaaaattcgt
    16261 cgatgggtcc ggtataaaac acatgattgt aaccgctcaa gtccatttta ggcgaaaatt
    16321 tagcattcaa ttgaacttta atatttgttc tgtcgagcat tctgttgacg atttccgtat
    16381 aaccttcggc cggaataccc tgaaattggt cgtcatagta attgtcatca taattgaacc
    16441 gaaccggaag ccgtttcaga atgcttgcag gcaacatatc gggttcacaa ccccattgtt
    16501 ttttcgtata tccgtagaaa aatgcttcgt aaagttcgcg tccgatgaat ttcaacgcct
    16561 gctcttcgaa attccgggga ttttcaatcg acttgtcggc tttatgttca atgtaattcc
    16621 gggcttcctg cggattcatc gctttgcctg caaactgatt gatagtatgc agattaacag
    16681 gaatagaata aacttttcct ttagatacgg ttttcacccg gtttataaac ggcttcatat
    16741 cgcaaaaccg gcagatgtac tcccaaacct ctttatcgtc ggtatgaaaa atatgagggc
    16801 catagcggtg tatcatcaca tctgtttccg cgtcacgttc ggtgtagcaa ttgcctccga
    16861 catgcgagcg ttcatccatc acgatgattt cgttatcagt atcgcggctc agttgttcag
    16921 ctacaaccgc tcctgaaaat ccggctccta caatcaggta tctcatttgt cctgcgaatt
    16981 attcttggta aaaatatttc ttgctacgct aactgcaaat tttggattaa aatatttctt
    17041 ctcaaagata gaaagattat ttttccttat caaataatac atataaaccg tcacaaagct
    17101 ttcaataatg atggtattcc aggctgtacc cacgaaaccg taaaatttac tcatcaggat
    17161 gttgagtaaa agaccaataa tactggctgt ggctgtggct ttgaaaaatg ttttgtccat
    17221 attcaaattc agcatggttt gaataccaaa aacattgctc aaactaataa taaacggagt
    17281 aaaagccata atccggaagg acagaacagc atcgtgaaat tgatttccat aaaacaggtt
    17341 tatgataagc ggcgcaaaaa taaacaggac aattccggca aaaaaagtca aatagcttat
    17401 catgggtaat acttttcgga taacgtccag gcctgtttct ctgtttttgg caaatgagtt
    17461 tccaataaag gggaaaatag ctgacgaaac cggaatgctt atcacactgt ttataacatt
    17521 cagcaagctt aaactcaccg taaaataacc gacctcggct gctgaaacaa atagtcccaa
    17581 tacaacagta ttggttgtgg tatataatga aataacaaca gtagaaataa agataactct
    17641 ctcctcataa agcagtcgga tagaactctt tattttcacc caatgaaagg tcagattgaa
    17701 tttccggcag gcatacaaca aaagaaaaac cgaacctaac agcgcgacag ttacgttgat
    17761 ggcaggcaaa atcacataat cattccgggt ttttataaat acaaaaacaa gtatggtact
    17821 tatcagccct ttcaaaaaat tcagaaaagc gaaaatcgag agatgttgct ttccctgata
    17881 aatgtattga ggaactaaaa ccaccgaaac agttgacaag aagagaatta gagtaacgta
    17941 aatattattt tgcagcggtt gaataacaag aatacaaatc aaaaaaagca aagcgctaat
    18001 taaaaaaagc aatatacgtg cgtttacaat ttgagaaaaa atagtgctga cataatcatt
    18061 atcattggga tttcgcgcaa tttttcttgt agcagtcaga tcaaatccat accctatcag
    18121 cagcgtgaaa taggcaataa aagcggtggc ataattgata attccgtaac tttccggtcc
    18181 tataattcgt gaaacataag gaactgtaat tagcggaaag acataattca ctaattgtac
    18241 tgttcctaat gaaaatatat tttttaaaag ttgtgtcttt tgcatgggcc gaaattaagc
    18301 aggcgaatac ctcacaaatc atttattgaa ctttttcaaa tacaaagtta tcattattat
    18361 tcattttacc tttccttttt cagattattg attgtatttt tatcagttta aatgaagtct
    18421 tgactttccg gaactacgta tgccatgatt gtttgttcgt tgttcaacat tgttatttcc
    18481 tgtttttact ttcggaaaga tatgattaaa gacagtcagc aggaatgaaa aattatatca
    18541 tgctacagcg tggaatttca aattaaaatc tgcgtttttt gttgataaaa aaggccaact
    18601 tttatgcaaa actggccttt tgagaaaaaa ttttctgatt aatctgaaat ataaggccaa
    18661 ccttcgttgt cccagtttaa cttgtaaatc aataatgttg gtcttccccg gtaagtcttt
    18721 tcataaccgt gcagaaacat atagtcggca ccaccgaaag tatagaccga attatgtcct
    18781 ttaccgtaaa attgttcatc gcttccggca atgagtgttc caccgccatg aagcatcgat
    18841 tttccttctt tatcaatata aggtcctgcc agtttttcgg aacgtcctac acgcacgttg
    18901 tagttactct tttcgccccg gcagcagagg tcaaacgagg caaaaagata gtaatatttt
    18961 ccttttctga aaacaaacgg agcttctatg gatgcatcac ccgggtctgt ttcatcgagc
    19021 atcttatccc gttgacgtga agctaccgta taccacgctt cgggcttggc tatggaaagt
    19081 aaatcgtcgg taagctgtac cattttcaga cctccccaga acgaaccaaa cattaaccag
    19141 ggttttccgt tttcgtcgaa aacaaggttc ggatctattg cattccagtt atcgcgcccg
    19201 ggaacggatt ctatcacttt accatgatcg acccatttat aacgcgggtc ggatggattc
    19261 agcgtaacat tggttgccac acctatacag gaacggttac tggcaaatga cgatatggaa
    19321 taatagagga aatattgccc gttgtgaaac gaaatgtcag gagcccaaat atttccatca
    19381 aatttgggaa cttctttctt cgcccattcg ggtgaaacat cgaaaacggg tttcataaac
    19441 tgccagtttt tcagatcgtt cgatttc
//
LOCUS       DAOPYB010000040        19168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_2989_length_19168_cov_8.650709, whole genome shotgun sequence.
ACCESSION   DAOPYB010000040 DAOPYB010000000
VERSION     DAOPYB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19168
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2989_length_19168_cov_8.650709"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..900
                     /gene="efp"
                     /locus_tag="PKH58_04885"
     CDS             334..900
                     /gene="efp"
                     /locus_tag="PKH58_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNX88394.1"
                     /translation="MATTADIKNGMCIDMDGQYYYVVEFLHVKPGKGPAFVRTKLKNV
                     VSGRTIDRTFSSGTKLDEVRIERRPYQYLYQDDMGYMFMNNETYEQIPIPKDQITGVD
                     FLKEGMEIEVVSHADSETILYAELPVQVNLLVTYTEPGLKGDTATNATKPATVETGAT
                     VRVPLFINEGETIRVDTRDGSYVERVKA"
     gene            complement(963..1487)
                     /locus_tag="PKH58_04890"
     CDS             complement(963..1487)
                     /locus_tag="PKH58_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNX88395.1"
                     /translation="MKLRKSVIFIILLFAVGIAGCNEKPPSLHISTSPETAAGGEFRN
                     VYKVIDGDTFWMKDRDGRIEKIRLIGIDAPEAHKSKHKDVQRFGRESKEFLKQFLKSR
                     KVALEYDVQKTDQYGRTLAYVYLEDGTFLNDYLIRYGYARVATFPPNVKYQKQLLASQ
                     KHARRQSMGMWKDR"
     gene            complement(1606..2808)
                     /locus_tag="PKH58_04895"
     CDS             complement(1606..2808)
                     /locus_tag="PKH58_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNX88396.1"
                     /translation="MSLKFLQEISSGTKNAYLKKDIICYYINNGENTLADLGKEMNLS
                     VPTVTKLVSELMDDGFVLDFGKQETNGGRRPSIYGLNENSGYFVGVDIKIFYINIGIV
                     NFKGEMVDSQMKIPFQMDNKHESFDKLCDIIDNYLKQEIVPREKILSIGINISGRVNT
                     QSGHSYSFFYFDERPLTDIFEERFGINISIDNDSRAMAYGEYMKGCVSGEKNVLYVNV
                     SWGLGLGIIINGQLYYGKSGYSGEFGHMTAFDNEVMCHCGKKGCLETEASGTYLHRKF
                     LEKLNNGYSSSLTKKTDKNIVLEDIINAALHEDILAIEIIEEIGNTLGKHIAGLINLL
                     NPELVVIGGTLSEAGDYLFLPLRSAIKKYSLNLVNKDTAIKLSKLGDKTGLIGACMLA
                     RSKMIGLI"
     gene            3026..4912
                     /locus_tag="PKH58_04900"
     CDS             3026..4912
                     /locus_tag="PKH58_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX88397.1"
                     /translation="MKKFFKNIYWFTFLFISCTGIYAAKSTYTDVLIIGGGASGTSAA
                     VKSSRLGVKTILIEESSWLGGMLTSAGVSAIDGNNKLPSGFWGEFKDSLINYYGGESK
                     LATGWVSNVQFEPSAGNRIFQNIARKEKNLSVYFNSSLLNVSQKNNQWIARIKTPNGE
                     KTIKSKVLIDATELGDVAKMTGVKYDIGMESRFVTGEPAAPEKANDIIQDLTYVAILK
                     DYHKDVTIPRPKDYDSTLYICSAKNPLCTNPEYPQYVWTPDKMITYGRLPNNKYMINW
                     PAEGNDYYVNIIEMTPAQRTEALKKAKSITTGFLYFIQKELGMNTLGMADDEYPTDDK
                     MPYIPYHRESRRIHGLVRFTENHLLNPYDQSEKLYRTAIAVGDYPVDHHHRRYTGNEN
                     IPQLYFHPVPSYGLPLGTLIPQNVEGLIVAEKSISVSNIVNGTTRLQPVILQIGDAAG
                     TLAALAVKQNKRIQDVSVRDVQNSLLEKGGYLLPYLDVPKSHPAFLSLQRVGSTGILK
                     GVGKNAGWSNETWFRADDILRMNDLEGLKDLYPDVNIIRNDSSEMYMKDLLVIVNKIM
                     HKRKSAKVEIESVVKDFFEQKGFQFDQNKKISRIEAALVIDKILDPFNAKTVNMKGEF
                     FENK"
     gene            4920..6032
                     /locus_tag="PKH58_04905"
     CDS             4920..6032
                     /locus_tag="PKH58_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HNX88398.1"
                     /translation="MKTTNRRNFIKKAAIATTSAITLPQLMGWEDAKASELKQSKLIK
                     KDVSLQSKLIVPQNNGLRITGTFLDEISHDIPHQNWGEAEWDLDFQHMKAIGIDTVIL
                     IRSGYRKFTTYPSKYLLSKGCYKPSVDLLDMYLRLADKHGMKFYFGLYDSGHYWDTGD
                     LSYEIEDNKYVIDEVWQNYGSKHKSFSGWYISGEISRATKGAIQAFYAMGKQCKDLSD
                     GLPTFISPWIDGKKAVAASGSKLTKEEAVSVQQHEKEWDEIFDGIHKVVDACAFQDGH
                     IDYDELDAFFTVNKKLADKYGMECWTNAETFDRDMPIRFLPIKFDKLRMKLEAAKRCG
                     YDKAITFEFSHFLSPQSAYSQAGNLYNRYKEYFNIR"
     gene            6034..7434
                     /locus_tag="PKH58_04910"
     CDS             6034..7434
                     /locus_tag="PKH58_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNX88399.1"
                     /translation="MKKNENIRYVAFLSFVAALGGFLFGYDTAVISGTVIQVTAQFNL
                     TTLQSGWYVGCALIGSIIGVSIAGQMSDKFGRKPTMIIAAILFFLSGIGCAISSNLDL
                     LVISRIIGGVGIGIVSIVCPIYISEISVAAYRGRMVSLYQLAVTIGFLGAYLMNYYLL
                     SVSHSFQSNNELFQKIFHTEVWRAMLGAEALPALLFMIVIFLIPESPRWLIVRSRELE
                     AVKTLNNIYRNKDMVQFQVNETKEMIRGEVKSDWKVLFEPWIFRAVLIGSAIAILGQF
                     MGVNAVLYYGPTIFENSGLSSGDSLFYQSLIGVVNVLTTILAMFIIDKAGRKKLVYFG
                     VSGMILTLILIGLYFFKGPKMGLSNVFLLVCFLMYIFFCAGSISAVIFVFLSEMYPTK
                     ARGLAMSVAGLSLWVGTFLIGQLTPWMLSNLTSAGTFWLFAVMCIPYMIIVWKLMPET
                     AGKSLEDIERFWMKKK"
     gene            7473..8645
                     /locus_tag="PKH58_04915"
     CDS             7473..8645
                     /locus_tag="PKH58_04915"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNX88400.1"
                     /translation="MEWEKLSLQYRDELLKNVIPFWLEKSQDKEYGGYFTCLDRQGNV
                     FDTDKFIWLQGREVWMFSMLYNKVEQRKEWLDCAVQGGEFLKKYGHDGNLNWYFSLDR
                     KGNPLVEPYNIFSYTFATMAFGQLSIATGNKEYADIAKKTFDTVLSKLENPKGKWNKA
                     HPGTRNLKNFALPMILCNLALEIEPLLDEKFLNRTIETCIQEVMDVFLRPELGGIIVE
                     NVELDGSLSDTFEGRLINPGHAIEAMWFVMDLGVRLQRKDLIDKAVETTLKMIEYGWD
                     EKYGGIFYFMDRLGHPTQQLEWDQKLWWVHIETLISLLKGYQLTGSQECLKWFEKVHD
                     YTWTHFKDPKHPEWWGYLNRQGEVLLDLKGGKWKGCFHVPRGMYQCWKTLERIAEK"
     gene            8787..9890
                     /locus_tag="PKH58_04920"
     CDS             8787..9890
                     /locus_tag="PKH58_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HNX88401.1"
                     /translation="MEGRRNFLKKVAVGSAGLLATQSIEGLNTVSAASISKKSEVRKT
                     NSDLTNKMFETPGILKGMPIKATFLDEISWDIPHQNWGVKEWDRDFMAMKNMGINTVV
                     MIRAGLGSWIASPFECILKTEDVFYPPVDLVEMFLTLADKHELAFYFGMYDSGKYWIE
                     GSFQKEIDLNMRLIDEVWNKYGHHSSFQGWYLSQEISRRTKNMSKIYAEVGKHAKAIS
                     NNLPTMVSPYIHGVKTDQVMSGDNALSVKEHEEEWDEILYSVKGAVDILAFQDGQVNY
                     NELFSYLQVNKKLADRYGMKCWTNIESFDRDMPIRFLPIKWEKLLLKLEAARKAGMEN
                     AITFEFSHFMSPNSAYSQAEYLYNRYKEHFKMK"
     gene            9925..13080
                     /locus_tag="PKH58_04925"
     CDS             9925..13080
                     /locus_tag="PKH58_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX88402.1"
                     /translation="MKRDKTNLLRWMSNRWIPLLFTFFLFCSTSLYAQVDVKGKILDE
                     KGESLIGVSVKVKGTTFGTTTNGNGEFSLRTPKSNSILEISYLGYQTQEANVQGKNFI
                     QITLQEETKSLDEVVVVAYGSQKKTSVTGAISTVSQRELLKAPTMSISNLVGARVAGV
                     SMLQQSGQPGSDAASLLVRGQGAIYVVDGVVRDFNEIDPNEIESVSILKDATSAALYG
                     LNASAVVLVTTKKGRADKLEISYTGSYGISQNANQLQWLDAPGYAYWYNKARELDGDA
                     TVFTNDQVQKMIDGKDGWGNTDWYKKLFDMGTNQHHNVSATGGNDKVRFFGSIGAFGQ
                     KGNVANFNYNRYNFRSNVDSKISKNFSLELGVSGRLEKRDNPRYSANPNDWHNIPQQA
                     VRALPYVAEKTTIDGKEYYTSTNTASSPVAPLAAINESGYARSNNTYIQTNFALKYDA
                     PWLKGLSFKFMGAYDIFQQFSKTLSVPFKTALAALPNSTTEKISYSIFKDNSGITSLT
                     ESAYSSTNAVTQSSANFDRTFDKHKINTLLLFETRNNFSNRLGVTGYGLDFLQLDELD
                     KITNKTANGDPKYPVPSGGSSQARVIGLVGRVNYEYNDKYLAEFSIRRDGTYLFWGNT
                     NSQWINIPAISLGWRVNKEDWFKADWVNNLKIRGGIGKTATSSVTPFQYLNLMAVSPN
                     SVIIGNSSQSLVYTKTLGNPNLSWESSINYNLGTDFSAWRGLLGIEFDVFYKYQYDIP
                     AYVAGAYPPSMGGYFFNVVNKNKIDYRGFDFTITHDNKIGDFQYGVKWLSSFAKRRWL
                     YYAGDSPNTPDYMKLTGKEVGSQLGFIAEGLFQSQEEIDNSATIPGAKVLPGYIKYQD
                     RNGDGKITYAQDMGYVGKSAYPKYQTSLNLNGAWKGFDFDILLQGGLGRTVALTGVYT
                     STGSEGIMDNTAFTKLFYHGGNSPQFLAENSWTPENTNAEFPRLSLVTVSSNNAYSST
                     FWYRSGDYLRFKTFQVGYSLPESLIKSWGVKQVRFYVEGSNIFTLSGLMKYNIDPEQP
                     GVNNGYYPQQKTFSGGIKITL"
     gene            13096..14712
                     /locus_tag="PKH58_04930"
     CDS             13096..14712
                     /locus_tag="PKH58_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX88403.1"
                     /translation="MKNILKITILAFILAGFYSCNDWLELTPTSDLTDKAVWENEESA
                     DLYVDGFYTYLSQYGQFGSQQFQGSLTESLTNTFKYGSYALGHKAGHPNNYVFNPSVI
                     TSASCFYDNWNDGYNKIRRMNEFLKSMQKYSTFSEEKNTLLEAQVRFFRAFMYFQLAK
                     RHGGVILYTDIDQMQKDMDRSTEKETWDLVESDLDFAIANLPGDWTSTADKGRLTKLA
                     AFAFKSRAMLYAQRWQSAYNAADSVILSNKYGLVDDYANACTGNNKEAILQFEYKVLG
                     PNHTFDKDYAPLSDGYEFGGLGTPTQEMVECYEKADGTKMDWSPYHSKTASRPPYENL
                     EPRFKASIIYPGSTWKGNVMQNSVNGTYGSFMAYRAQPYTYGYTTTGYFLRKLMDESH
                     KDLKGTPSTQTWVEIRYAEVLLNKAEAAYRLSKISEAQSAMNLVRARVGLPAKSSTGD
                     DWFKDYINERKVELAYEGQLFWDMRRWKLAHIEYNNYRCHGFKITGDNYEYIDVDYQN
                     RQFLEKTYILPIPDEELQNNSVIAQYDEWK"
     gene            14733..16391
                     /locus_tag="PKH58_04935"
     CDS             14733..16391
                     /locus_tag="PKH58_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5018 domain-containing protein"
                     /protein_id="HNX88404.1"
                     /translation="MKNKILSIFAIFTLLLGWNSCQQVEDLTPSISREGINSLTASFL
                     NDESSENSFTSEIDYANGVITIVFPYNYPRTSDNVLTMDKLKHIRVQANLDDNVTISP
                     ALLYMDLTQENYITVTDQTKKQKQYKVIGEIRKSNETKITSFELQTLGIAGVIDDNSK
                     IISLISADVIGDAIATVNVAHGANMNPDPTKTALNYDSDVKITVTAQDGKTTSVYTVK
                     KAAPSKTEFGMRAGSAKILWVKKLNSELGISTLNLTGGIAATKDYLVINTRGINSIYL
                     DRKNGEIAGTLNLGDKLGSLVNFYNTCDDNDNILLCNLSPNNGSFKVWKIKGVNGTPE
                     AFIEWNGGVAIGRKLSVKGSIDGDAIITGAILGAGNKFARWQVKGGVLLSQTPEIITI
                     SGIGGAWNNNCDIVSTSATDLSADYFAAYYAAPYQFAWVNGTSNSIKATSPVLSSNWI
                     SNAADYIVFNKCPYAVQNSINSFTWGSDDTVYLYDVSSTSTMGASVWKADIGKYGGKE
                     NGGQNANGTGDVALKVSDNGYYMYLYFMFTNGCVVCVQYDCISM"
     gene            16554..17945
                     /locus_tag="PKH58_04940"
     CDS             16554..17945
                     /locus_tag="PKH58_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase family protein"
                     /protein_id="HNX88405.1"
                     /translation="MKKYKINHHSLFLLIACCFIFSLQSCKDKKVPYDFPDPPSISNK
                     DKPRFIWIDAAANFKDFANSKENIARDLNLAKNAGFTDIVVDIRPTNGDILYQSSVEG
                     SKQVTWLPAWVNGVYSKVERTETWDYLQAFIDKGHELGLKVHAGFNTMVGGNGNGLGN
                     QGLLYRDNTKKSWATSENLTSGITNTMDNGDGTKFFNPANDEVQNYICNLLKDLAKYD
                     LDGIFLDRGRFDGIKSDFSDITRQKFQTYIGGSTITNFPEDILPKGATMQTISSMSNY
                     PVYFKQWLEFRAKIMYDFMEKAKNAIKSVNSKIKFGVYVGGWYSSYYEVGVNWGSKKY
                     NTSSYYSKWATANYQNYGYAGLMEQMLIGAYASPLRVYGTTEWTMQGFCKLAKEKTKG
                     DCPMVAGGPDVGNWDSNNTATQEEENQAIVNSVKACMDECDGYFLFDMIHLKMANQWI
                     YAKEGIDKTLTAN"
     gene            17948..>19168
                     /locus_tag="PKH58_04945"
     CDS             17948..>19168
                     /locus_tag="PKH58_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase family protein"
                     /protein_id="HNX88406.1"
                     /translation="MIKSKFSPIICIVSLLCVILPEHSKGQYISISGKVICNGKGVES
                     VVVTDGNTCVTTDKKGLYTIQTSKSTDFVYLSTPSGYLPHDSLMIPHFYKRLNFNKSK
                     IYNFELKKNPKNDYNHTLLVHTDPQFYKTGNFNHYQKVIEDCQETINKYKNVDVFGID
                     CGDLVGDKPEFYPTYIEYLSKTEIPFYRVLGNHDMDYSRRSDETSTTSYNKIFGPAYY
                     SFNKGKNHYIILDDIFYIGRDYFYMGYVSEKILNWLKEDLSYVPKGSTVFVSMHIPAR
                     LNNKSEFFKYDGYSIAEQTVNVSALFQVLEPYNVHILTGHMHYNRNIIHSSTLYEHNL
                     GAICGTWWQGDYCLDGSPIGYGVYEITGEQVNWYFKSVGYPRSCQMRAYPAGSNTEFP
                     DDIIVNVWNWDKNWK"
BASE COUNT         6394 a         3331 c         3728 g         5715 t
ORIGIN      
        1 agagctgcgt cttgagggct gcgcccgttt taccaacagc agaattatag tatccggcag
       61 gtatttgagc cgatagttga aataatgaaa aaagtaataa aagagtaaga tttcttttca
      121 taaatgtatt ttttgtgaat taaaaaaggg ttgcaaatat agcacctttt ttcagttatc
      181 cattattatt gcatattaaa cttatattaa acaaacgata atcttgcaga acagattacg
      241 ctgattatca atttgttttt ccatttcaaa gaaaatcatt aactttgcca cccgaaaaat
      301 ataagaataa tcttttaaaa aaatactttt tttatggcta caacagcaga tatcaagaat
      361 ggaatgtgca tcgacatgga tggacaatat tattatgtag ttgaattttt acatgtgaaa
      421 cccggaaaag gtcccgcttt cgtacgcaca aaactgaaaa atgttgtatc aggccgtact
      481 atcgaccgca cattcagttc aggtaccaaa ctcgatgaag tacgcattga gcgtcgccct
      541 tatcagtatc tttaccagga tgacatgggt tatatgttta tgaataatga aacttatgaa
      601 caaataccta tccctaagga tcagattaca ggtgtagatt ttctgaaaga aggtatggaa
      661 atcgaagtag tatcgcatgc cgattcggaa acgatacttt atgctgaact tcctgtacag
      721 gtaaatttac ttgtaactta taccgaaccc gggttgaaag gcgatactgc aacaaacgca
      781 acaaaaccgg ctacagttga aacaggagct acagtacgcg taccactttt tattaacgaa
      841 ggagaaacga tacgtgttga cacccgcgat ggttcatacg ttgaacgtgt aaaagcataa
      901 aaactttttg atcataaata aagccggtga ttatttctcc ggcttttttt tgtgcatata
      961 aattatctgt ctttccacat tcccatcgat tgccgacggg catgcttctg gctcgcaagc
     1021 agctgctttt gatactttac attcggagga aaggttgcca cccgggcata cccatacctg
     1081 atcagataat cattcaggaa agtgccgtcc tcgaggtaca catatgccag cgtacggcca
     1141 tattggtcgg ttttctgcac atcatattcc agcgccactt tccggctttt caggaattgt
     1201 ttcaaaaact ctttcgattc ccttccaaag cgctgaacgt ctttatgctt tgatttatga
     1261 gcttcaggag catcaatccc gattaaccga attttttcta ttcgcccatc acgatctttc
     1321 atccagaatg tatcaccgtc tatcactttg taaacattcc tgaattcccc gccagcagcg
     1381 gtttccgggg acgtggaaat gtgcaaagag ggtggtttct cattgcatcc agctatacca
     1441 actgcaaata agagaattat aaagataact gacttcctta atttcatgat cttatgatta
     1501 tttttcttag tgcaaaaata gcattttgcg gttgtaatca caaccaatga ataatcaata
     1561 aattaaaatc attaaggttg cattacaact tgaatcttgc taagattaaa taagcccaat
     1621 cattttgctt cgagctaaca tgcaagctcc tatcaacccg gttttatcac ctaattttga
     1681 aagtttgatg gctgtatctt tatttaccaa attgagagag tattttttta tggcacttcg
     1741 taacgggaga aacagataat ctcctgcttc tgaaagagtt ccacctatca caaccagttc
     1801 tggatttaac agattaatca atccggcaat atgcttgccc agcgtgtttc caatctcctc
     1861 aattatttct atagctaaaa tatcttcgtg taaagcggca ttaattatgt cttccaaaac
     1921 gatattttta tcagtcttct ttgtcaaaga agaagaatag ccattattta atttttccaa
     1981 aaatttccga tgtaaatacg tacctgatgc ttctgtttcg agacatcctt ttttcccgca
     2041 atggcacatc acttcattat caaaagcagt catatgccca aattcgccag aataaccgga
     2101 ttttccataa taaagctgtc cgttaataat aataccaagt cccaagcccc aactcacatt
     2161 cacgtataat acattttttt caccgctgac gcatcccttc atatattcac cataagccat
     2221 agccctggag tcgttatcaa tggaaatatt aatgccgaat cgctcttcaa atatatcagt
     2281 caggggcctc tcatcaaaat agaaaaaact gtaactatga ccggactgtg tatttacacg
     2341 tcctgagata ttaataccta tgcttaaaat cttttcgcgt ggaacaattt cttgtttcag
     2401 atagttatca attatgtcgc ataatttgtc gaaactttcg tgcttattat ccatttgaaa
     2461 cggaattttc atttgtgaat caaccatttc acctttaaaa tttactatcc ctatattgat
     2521 gtaaaatatt tttatatcta cacctacaaa atatcctgaa ttttcgttta atccgtagat
     2581 acttggacgt cttcctccgt tagtttcctg cttgccaaaa tccaggacaa aaccatcatc
     2641 catcagttcg gaaactaatt tagtaacggt tgggacactc aaattcattt ccttgcctaa
     2701 atcagctaat gtattttcac cattgttaat atagtagcag attatgtcct tttttaagta
     2761 tgcatttttc gtaccggaac tgatttcttg taaaaatttt aagctcatga cattattata
     2821 tgattaaaaa tatacaaatt tagaatatat aattaaatta ttatattgat tgaatattat
     2881 ttaactattt tcatctatct tcttttattt attaaaccgc aacatgttat gcaacatatt
     2941 tattaaattt ttttgttata tggacgtagt taataaatat atatttttat atttgcactt
     3001 tgatcatctt taaaaacaat ttgtaatgaa gaaatttttc aaaaacattt attggtttac
     3061 tttcctattt atttcttgca caggaattta tgctgctaaa agcacttata ctgatgtatt
     3121 aatcattggt ggcggagcga gtggcacatc ggctgctgtg aaaagttcac gattaggcgt
     3181 taaaacaatt ttgattgaag aaagctcctg gcttggcgga atgttgacaa gtgcaggagt
     3241 tagtgccatt gatggaaaca ataaacttcc aagcggattc tggggagaat tcaaagactc
     3301 tttgataaat tattatggag gtgaaagtaa attagctacg ggatgggtta gtaatgtgca
     3361 atttgagcca tccgcaggta atcgtatttt tcaaaatatt gcccgaaaag aaaaaaatct
     3421 atctgtttat tttaattcct ctctcttgaa tgtttctcaa aaaaataatc aatggatagc
     3481 tcgtattaaa actccaaatg gtgaaaaaac aattaaatca aaagtcctga ttgatgctac
     3541 tgaacttggt gatgtggcaa aaatgactgg tgtgaaatat gacataggta tggaaagtcg
     3601 cttcgtcaca ggtgaaccag ccgcaccaga aaaagcaaac gacattattc aggatttgac
     3661 gtatgttgcc attctaaaag actaccacaa agatgtaaca attccacgtc cgaaagatta
     3721 tgattcgaca ttatatatat gtagcgcaaa aaatccgcta tgcaccaatc ctgaatatcc
     3781 tcaatatgta tggacacccg ataaaatgat tacgtatgga agattaccga ataataaata
     3841 catgattaat tggcccgccg aagggaatga ctactacgta aatattattg agatgactcc
     3901 cgcccaacgc actgaagctc ttaaaaaagc aaaaagcatt acgacaggtt tcttgtattt
     3961 tatccaaaag gaattgggta tgaatacatt gggcatggct gatgatgaat atcctaccga
     4021 tgacaaaatg ccgtatattc cttaccacag ggaatcacga cgcatacacg gattggtacg
     4081 gtttaccgaa aaccatttgc tgaatcctta cgatcaatct gaaaaacttt atcgcacagc
     4141 aattgcggta ggtgattatc ctgttgatca tcatcaccga agatataccg gaaatgaaaa
     4201 catacctcaa ctttactttc atcctgtccc atcttacgga ttgcctttgg gcactctaat
     4261 tcctcaaaat gtagaaggat taattgttgc ggagaaatcc atttctgttt ccaatattgt
     4321 taatggaaca acccgtttac agcctgtgat acttcagatc ggtgatgctg cagggacatt
     4381 ggcagcactt gcagtaaaac agaacaaaag aatacaggac gtgtccgttc gtgatgtgca
     4441 aaattcgttg cttgaaaaag gcggatactt gcttccatat ctggatgttc caaaatctca
     4501 tcccgcattc ttatcgctgc aacgtgtggg ctcaacaggt atattgaaag gagtaggaaa
     4561 aaacgcagga tggtctaacg agacctggtt cagagcagat gatatattga gaatgaatga
     4621 tttagaagga ttaaaggatt tatatcctga tgtaaatatt attagaaacg attcgtccga
     4681 aatgtatatg aaagacttgt tggtcatagt aaacaaaatt atgcataaaa gaaaatcagc
     4741 gaaagtagaa attgaatctg tcgtaaaaga tttttttgag caaaagggat ttcaatttga
     4801 ccagaataaa aaaataagcc ggatagaggc agccttagtg atagataaga ttttagaccc
     4861 attcaatgct aaaaccgtca acatgaaagg tgaatttttt gaaaataaat aaaaatcgta
     4921 tgaaaacaac taaccgacgt aattttatta aaaaagcagc cattgccacc acctctgcaa
     4981 ttaccttgcc gcaactcatg ggttgggaag acgcaaaggc cagtgaatta aaacaatcaa
     5041 aattgataaa aaaagacgtg tctttacaaa gcaagttgat cgttcctcaa aataacggat
     5101 taagaattac cggaacattt ctggatgaaa tctcacacga tattcctcat cagaattggg
     5161 gtgaagctga atgggatttg gattttcagc acatgaaagc catcggtatt gatacagtta
     5221 ttttgattcg ttcgggatat agaaagttca cgacttatcc gtccaaatat ctgctttcta
     5281 aaggatgtta taagccctcg gtggatttac tggatatgta cctgcgtttg gccgacaaac
     5341 acggaatgaa attctatttt gggctttacg actcaggcca ttattgggat acaggcgatt
     5401 tgagttacga aattgaagat aataaatatg tcattgatga agtatggcaa aactacggga
     5461 gtaaacataa aagctttagt ggttggtaca tcagcggcga aataagcaga gctacaaaag
     5521 gtgcaattca ggcattttat gcgatgggaa aacaatgtaa agacctttct gatggattac
     5581 ctacattcat atcaccctgg attgacggga aaaaagcagt tgcagctagt ggcagtaagc
     5641 ttaccaaaga ggaagcagta tcggttcaac agcatgaaaa agaatgggat gaaatttttg
     5701 acggaattca caaagtggtt gatgcttgtg catttcagga cggacatatt gactacgatg
     5761 agttggatgc cttctttaca gtaaataaaa aattagcaga taaatacggc atggaatgct
     5821 ggacaaatgc agaaacattt gaccgagata tgcctatacg tttcttaccg ataaaatttg
     5881 ataaactgcg aatgaaatta gaagctgcca aacgttgcgg atacgacaaa gccattacgt
     5941 ttgaattttc gcatttctta agcccacaat cggcgtattc gcaagccgga aatctgtata
     6001 atcgctacaa agaatatttt aatataagat aatatgaaaa aaaacgaaaa catacgatac
     6061 gttgcctttc tttcatttgt tgccgcactt ggaggttttt tattcggtta tgatacagct
     6121 gtaatttcag gtactgtaat ccaggttact gctcaattta atttaaccac ccttcaaagc
     6181 ggttggtatg ttggttgtgc tttgattggt tcaattatag gcgtatccat agcaggtcaa
     6241 atgagtgata aattcggcag aaaaccgaca atgattattg ccgctatcct gttcttctta
     6301 tccgggatag gttgtgccat ttcatccaat ctggacttat tagtaattag tcgcattata
     6361 ggcggtgtgg gtattggtat agtgtctatt gtttgcccaa tttatatttc cgaaatatca
     6421 gttgcagctt atagaggcag gatggtctcg ctatatcaat tagcggtaac aatcggattt
     6481 cttggagcgt atctgatgaa ctattatttg ttgagcgtat ctcactcttt ccaatcaaat
     6541 aatgaattat ttcaaaagat atttcataca gaagtttggc gtgccatgct tggagcagaa
     6601 gctttgcctg ctctcctgtt catgatagtc atctttttaa ttcctgaaag cccgcgttgg
     6661 ttaattgtcc gttcacgcga actagaagcc gtgaagacac tcaataatat ttacaggaat
     6721 aaagatatgg ttcaattcca ggtgaatgaa accaaagaaa tgattcgggg agaagtaaaa
     6781 tcggattgga aagtactgtt cgaaccgtgg attttcagag ccgtacttat tggatcggct
     6841 attgccatac tgggacaatt tatgggcgtg aatgctgtgc tatattatgg acctaccatt
     6901 tttgaaaata gcggattatc cagtggtgat tctcttttct atcaatcatt aatcggtgta
     6961 gtcaacgttc tcacaactat tttagcaatg tttatcatag ataaagcagg taggaaaaaa
     7021 cttgtttatt tcggagtatc agggatgata cttacactta tcctgattgg actttacttt
     7081 ttcaaaggac cgaaaatggg actctccaat gtatttctac ttgtttgctt cctgatgtat
     7141 atttttttct gtgcaggctc tatcagtgcg gttatatttg tctttttatc cgaaatgtat
     7201 cctactaaag cacgtggttt agcaatgagt gtggcaggac tttcactttg ggtgggaaca
     7261 ttcctgatag gacaactaac cccgtggatg ctttcaaatc tcacttcggc aggtactttt
     7321 tggttatttg cagtgatgtg tatcccttac atgatcattg tttggaaact gatgcccgaa
     7381 acagccggaa aatctttgga agatattgag cgtttttgga tgaaaaagaa ataattaata
     7441 aatgtgcttt tactgaaaaa tattgatttt ttatggaatg ggaaaagtta tcattgcaat
     7501 accgcgatga attattaaag aatgtgattc ctttttggtt ggaaaaatca caggacaaag
     7561 aatacggagg ttatttcacc tgtttagaca gacagggcaa tgtatttgac accgataagt
     7621 ttatctggct tcagggacgt gaagtttgga tgttttcgat gctttacaac aaagtagagc
     7681 aacggaaaga atggcttgat tgtgctgtac aaggtggaga gtttctaaaa aaatatgggc
     7741 acgatggcaa tcttaactgg tatttctcac ttgaccgcaa agggaatcca ttggtcgaac
     7801 cgtacaatat tttttcttac acctttgcca caatggcatt tgggcagttg agtattgcaa
     7861 cgggaaataa ggaatatgcc gatattgcaa aaaaaacctt tgatacagtg ctttccaagt
     7921 tggaaaatcc aaaaggaaaa tggaacaaag ctcatcccgg cactcgcaac ttgaaaaatt
     7981 ttgcattacc gatgatactt tgtaatctcg ctctggaaat tgaaccgtta ttggacgaaa
     8041 aattcctgaa tagaaccatc gaaacttgta ttcaagaagt gatggatgtt tttctgcgtc
     8101 ctgaacttgg aggaataata gtagaaaatg ttgaattaga cggtagttta tcagatacgt
     8161 tcgaaggcag attgataaat cccggacatg ctattgaagc catgtggttt gtaatggatt
     8221 taggagtgcg tttacagaga aaagatttaa ttgataaagc cgtagaaaca accctgaaga
     8281 tgatagaata cggctgggat gaaaaatacg gtggcatatt ctattttatg gaccgtctgg
     8341 gacatcctac tcagcaattg gaatgggatc agaaactttg gtgggtgcat attgaaaccc
     8401 ttatatcact gctgaaaggt tatcagctta caggttcaca ggaatgctta aaatggtttg
     8461 aaaaagttca cgattataca tggactcact tcaaagatcc aaaacatccc gaatggtggg
     8521 gatatttaaa tcgtcagggt gaagtattac tagacttaaa aggaggtaaa tggaaagggt
     8581 gtttccatgt accaaggggt atgtatcagt gctggaaaac tctggaaaga attgctgaaa
     8641 aataacatca ttaaaacaat aactatgtat agcatttaat tcaaattaaa tcggaaggaa
     8701 aaattgtaaa aaacaaaaat atttttgatc caattacttt ctcaaaaatt gtcgaacaac
     8761 tctattttag ttaataaaaa atgaatatcg aaggaagaag aaattttctc aaaaaagttg
     8821 ctgtcggatc agcaggatta cttgcaacac aatctattga aggattaaac acggtatcgg
     8881 ctgcatcaat cagtaaaaaa tcggaagttc gtaagaccaa ttctgatctt acgaacaaga
     8941 tgttcgaaac tcccggaatt ttaaaaggca tgcctataaa agccaccttc ctggatgaga
     9001 taagctggga tattcctcat caaaactggg gtgtcaaaga gtgggacagg gattttatgg
     9061 caatgaaaaa catgggaatc aatacggtgg tgatgattcg tgcaggatta ggttcatgga
     9121 tagcatcacc gtttgaatgc attttgaaaa ccgaagacgt attttaccca ccggtagatt
     9181 tggttgaaat gtttttaaca ttagctgata aacatgaact ggctttttac tttggcatgt
     9241 atgattccgg aaaatattgg attgaaggtt cttttcaaaa agaaatagat ttgaacatgc
     9301 gcctcataga cgaagtctgg aataaatatg gacatcacag ctcgtttcag gggtggtatc
     9361 tctcgcagga aatcagccgt cgtacaaaaa atatgtcaaa aatttatgct gaagtgggca
     9421 aacacgccaa agccatttct aacaatctac caacaatggt ttctccctac attcacggtg
     9481 taaaaaccga ccaggttatg agtggtgata atgcattgag tgtgaaagaa cacgaggaag
     9541 agtgggatga aatactctac agtgtaaaag gagctgtcga cattcttgca tttcaagacg
     9601 gacaggtaaa ttacaatgag cttttcagct acctgcaggt aaataaaaaa ttagccgatc
     9661 gttatggcat gaagtgttgg acaaacattg agtcatttga ccgtgatatg cctattcgtt
     9721 tcttacccat aaaatgggaa aaacttctgc taaaattaga agcagctcgt aaagcaggaa
     9781 tggaaaatgc catcacgttt gaattctcac actttatgag ccctaattca gcatattctc
     9841 aagcggaata tttatataat agatacaaag aacatttcaa aatgaaatag ttgactaaat
     9901 taacaacatt aaattaaatt attcatgaaa agagacaaaa ctaacttatt gaggtggatg
     9961 tcaaaccgtt ggataccact attgtttact ttctttcttt tctgctctac atcactttat
    10021 gctcaagtag atgtaaaggg gaaaatctta gatgaaaaag gagaaagttt aatcggcgtg
    10081 agtgtgaaag tgaaaggaac tactttcggc actactacta atggcaacgg agaattttct
    10141 cttagaactc ctaagtcaaa ttctatcctt gagatttcat acctgggata tcagacacag
    10201 gaagcaaacg tacaaggtaa aaatttcatt caaattactc tgcaagaaga gactaaatcg
    10261 cttgatgaag tggtggtggt agcttatggt tcacagaaaa aaacaagtgt aacaggcgca
    10321 atttctacag tatcgcaaag agaacttttg aaagccccga caatgagcat ttcgaattta
    10381 gtaggtgccc gagtggctgg agtgagtatg cttcagcaat caggacaacc cggtagtgat
    10441 gccgcttcat tattagtcag aggacaagga gcaatatatg tggttgatgg tgttgttcgg
    10501 gattttaatg agattgatcc gaatgaaatt gaatctgttt ctattttaaa agatgctaca
    10561 tcggcagctt tatatggatt aaatgcttct gctgtagtgc ttgtaactac caaaaaagga
    10621 cgagctgaca aattggaaat tagttatacc gggtcatacg gaataagcca aaatgccaat
    10681 caattacagt ggttggacgc gccgggttat gcttattggt ataacaaagc ccgtgaacta
    10741 gatggcgatg ctactgtatt tacaaatgac caagtgcaga agatgataga tggtaaagac
    10801 ggctggggca atacggattg gtataaaaaa ttattcgata tgggtacgaa tcagcaccac
    10861 aatgtaagtg caactggagg gaatgataaa gttcgcttct ttggttctat cggagcgttt
    10921 ggtcaaaaag gaaacgtagc caattttaac tacaatcgtt acaacttccg ttcgaacgta
    10981 gactctaaaa ttagcaaaaa tttttcatta gaattaggag tatcagggcg tcttgaaaaa
    11041 agggacaatc cacgctactc tgctaatccg aacgattggc acaacattcc acagcaagcg
    11101 gtaagagctc taccttatgt agccgaaaaa acgactattg atggaaaaga atattatacc
    11161 tcaacgaata cagcctcttc accggtagct cctttggctg ctataaacga atcgggttat
    11221 gcaagatcta acaacacata tattcagaca aactttgccc ttaaatatga tgctccctgg
    11281 ttaaaaggat taagcttcaa atttatgggt gcatatgata tcttccagca attctcaaaa
    11341 acactttctg ttccattcaa aactgcatta gcagcattac caaattcaac tacagaaaaa
    11401 ataagctata gcatttttaa agacaacagc ggcattacaa gcttgacaga atctgcctac
    11461 agttcaacaa atgcagtaac tcaatcaagt gcgaatttcg ataggacatt tgacaagcac
    11521 aaaattaaca cactcttgtt gtttgaaaca agaaataatt tttcaaatcg attaggtgtt
    11581 actggttatg gattggattt tttacaatta gatgaactgg ataaaataac aaataaaact
    11641 gcaaatggtg atcctaaata tcccgttcca tcaggcggaa gcagtcaggc tcgcgtgata
    11701 gggttggtcg gtcgtgttaa ttatgaatac aatgataaat atcttgctga attttctata
    11761 agacgcgacg ggacttattt attctgggga aacaccaatt ctcaatggat taatatacct
    11821 gctatctcat taggatggag agtaaacaaa gaagattggt ttaaagcaga ttgggtgaat
    11881 aacttaaaaa ttagaggtgg tattggaaaa acagcaacct caagtgttac cccgttccag
    11941 tacctaaatt taatggctgt ttctccaaat tctgttataa taggtaattc aagtcaaagt
    12001 ttagtttata caaaaacatt aggtaatcct aatctgagtt gggaaagtag tataaactac
    12061 aatttaggta cagatttttc agcttggcga ggattattag ggatagaatt tgatgtattt
    12121 tataaatatc aatatgatat tcctgcttat gtcgctggtg cttatcctcc atctatgggc
    12181 ggctacttct ttaatgtcgt aaataaaaac aaaatagact atcgaggttt tgattttacg
    12241 atcacacacg acaacaaaat tggcgatttt caatatgggg taaaatggtt aagttcgttt
    12301 gcaaaacgtc gctggttata ttatgctggc gattctccaa atacacctga ttatatgaaa
    12361 cttacaggaa aagaagtagg ctctcaactt ggatttatag cagaaggact tttccagtca
    12421 caagaagaaa ttgacaattc tgcaaccatt cccggtgcaa aagtactccc cggatacatt
    12481 aaatatcagg atagaaacgg tgacggaaaa attacgtatg cacaagacat gggctatgta
    12541 ggtaaaagcg cctacccaaa atatcaaaca tcattgaacc taaatggtgc gtggaaagga
    12601 ttcgattttg atatattatt acaaggaggc ttaggacgca cagtcgcatt gacaggcgtt
    12661 tatacttcta ctggttctga aggtattatg gataatacgg cgtttacaaa attattctac
    12721 cacggaggga actcgcctca gttcttagct gaaaatagct ggacacccga aaatacaaat
    12781 gcagaatttc ctcgtctatc tttagttaca gtaagttcca ataatgctta ctcatctact
    12841 ttctggtatc gttccggaga ttatttgaga tttaaaacat tccaggtcgg atattcactc
    12901 cctgaaagct taatcaaatc gtggggagta aaacaggtaa gattttatgt agaaggatct
    12961 aatatattca cattaagcgg attgatgaaa tacaatattg accctgaaca acctggtgta
    13021 aacaatggat attatccgca gcaaaaaact ttttcaggcg gtattaaaat tacattataa
    13081 ttaaaattga aaacgatgaa aaatatactt aaaataacaa ttctggcttt tatacttgcc
    13141 ggattttata gttgtaatga ttggctagaa ctcactccta ccagcgatct taccgataaa
    13201 gctgtttggg aaaatgaaga aagcgccgat ctgtatgtag atggtttcta cacctattta
    13261 agccagtacg ggcaatttgg atcacagcaa tttcagggta gtcttacgga aagcttgaca
    13321 aataccttca agtatggttc ctatgcactt ggacataaag ccggacatcc gaataattat
    13381 gtatttaatc cgagtgtaat cacatctgca agttgttttt acgataattg gaatgatggc
    13441 tacaataaga ttagaagaat gaatgaattc ttaaaatcaa tgcagaaata ttccactttt
    13501 tcagaagaaa aaaatacact attggaagca caggtacgtt tctttcgtgc atttatgtat
    13561 tttcagcttg ccaaacgaca tggcggcgta atcctttata ccgatattga tcaaatgcag
    13621 aaagatatgg accgcagcac cgaaaaagaa acgtgggatt tagtagaatc tgatttagat
    13681 tttgccattg ccaatttacc cggtgattgg acatctactg cagataaagg gcgtcttaca
    13741 aaacttgcag catttgcctt caaatccaga gcaatgttgt atgcacaacg ctggcaatca
    13801 gcatataacg cagccgattc agtaattctg tcaaacaaat acggtctcgt ggatgactat
    13861 gctaatgctt gtacaggaaa taacaaagaa gccattcttc aatttgaata caaggtttta
    13921 ggtccaaatc atacttttga caaagattat gcaccattaa gtgatggtta tgagtttggt
    13981 ggactgggta ccccaaccca agaaatggta gaatgttatg aaaaagccga tggcactaaa
    14041 atggattggt caccttatca ttccaaaaca gcatctcgtc ctccctacga aaaccttgaa
    14101 ccgcgattta aagcatccat tatttatccc ggcagcactt ggaaaggtaa tgtaatgcaa
    14161 aattcagtaa acggaactta cggaagtttt atggcttaca gagcacaacc atatacttat
    14221 ggatatacca ccacgggcta tttcttaaga aaattaatgg atgaaagtca taaagacctg
    14281 aaaggtactc cgagcactca gacttgggta gaaatccgtt atgcagaagt acttctgaac
    14341 aaagctgagg cagcttatcg gttaagtaaa atttccgaag cacaatcagc tatgaattta
    14401 gtacgcgccc gggttggctt acctgctaaa tcttctaccg gtgatgattg gtttaaagat
    14461 tatataaatg aacgtaaagt agaactagct tatgaaggac aactattttg ggatatgcgc
    14521 agatggaaat tagctcatat agaatataat aattatcgtt gtcatggttt caaaattaca
    14581 ggggataatt atgaatatat tgacgttgat tatcaaaacc gtcaattcct ggaaaaaacg
    14641 tatatacttc ctatccctga tgaagaattg caaaacaact cagttattgc ccaatacgat
    14701 gaatggaaat aataaccgta aaatataaaa caatgaaaaa taaaatttta tcaatatttg
    14761 ctattttcac ccttctattg ggttggaata gttgccaaca ggtggaagac ctcactccat
    14821 cgatttcacg agaagggatc aacagcctca cagcgtcctt cctcaacgat gaaagttctg
    14881 aaaattcatt tactagtgaa atcgactatg ctaatggtgt tattactatt gtttttccct
    14941 ataactatcc tcgtacatcc gataatgttt taacaatgga taaattaaaa catataagag
    15001 tgcaggctaa cctggatgat aatgtaacca tttctccggc attattatac atggatttaa
    15061 cgcaggaaaa ttatataacc gtaacagacc aaacgaagaa acaaaaacag tacaaagtaa
    15121 taggagaaat cagaaaaagt aatgaaacca aaattacaag cttcgaactc caaacccttg
    15181 gtattgcagg agtaattgac gataattcga aaataatttc tcttatttct gccgatgtta
    15241 taggcgatgc cattgccact gtaaatgttg ctcatggagc taatatgaac cctgacccta
    15301 caaaaacagc attgaattac gacagtgatg tgaaaataac tgttacagct caagacggta
    15361 aaacaacttc cgtttataca gttaaaaaag ctgctccttc caaaacagaa tttggaatgc
    15421 gtgcaggaag tgctaaaata ctttgggtga aaaagttgaa ttctgaactt ggtatcagta
    15481 cattaaattt gactggtggt attgcagcta caaaagatta tttggtaata aataccagag
    15541 gaattaacag tatttatctt gaccgaaaaa atggagaaat agccggcact cttaatttag
    15601 gagataagtt aggaagcttg gttaacttct acaatacttg tgatgataat gacaacatct
    15661 tactttgtaa tttatcacca aataacggtt catttaaagt ttggaaaatc aaaggagtta
    15721 atggaactcc ggaagcattt atcgaatgga atggcggagt tgctattgga cgtaaacttt
    15781 cagtaaaagg aagcattgac ggtgatgcca ttattacagg tgcaatttta ggagcaggaa
    15841 ataaatttgc ccgttggcag gtaaaaggag gagtactttt atcacagact cccgaaatta
    15901 taaccattag tggtattggt ggggcatgga ataataattg tgacatagtt tctacaagtg
    15961 cgacagattt aagcgctgat tattttgcag cttattatgc cgcaccctat caatttgcct
    16021 gggtaaatgg cactagcaac tcaattaaag ccacatctcc ggttttaagc tccaactgga
    16081 tttctaatgc agctgattat attgtattta acaaatgccc atacgcagta cagaattcta
    16141 taaattcatt tacctgggga agcgatgata cagtttatct gtatgatgta agttccacca
    16201 gcactatggg cgcttctgta tggaaagctg acataggcaa atatggcgga aaagaaaatg
    16261 gaggacaaaa tgcaaacgga acaggtgacg tagccttgaa agtttcagat aatggatatt
    16321 atatgtatct gtactttatg ttcaccaacg gttgtgtagt ttgtgttcaa tatgattgta
    16381 ttagtatgta aatttggcaa taaaacctgc accaatatct ttggtgcagg ttatttcttt
    16441 ttatatgaaa caagcatgaa ccaacggtca acatagttat tacaaacttt ctccaaagac
    16501 attgaaaatc agtcatacaa gttccacacg ataattaaaa aaaatattta cggatgaaaa
    16561 aatataaaat caatcatcat agtttatttc tgttgatagc ctgttgtttt attttttctt
    16621 tgcagtcttg taaagacaaa aaagttcctt atgattttcc cgaccctcct tctattagca
    16681 ataaggacaa accacgtttt atctggattg acgcagctgc aaattttaaa gatttcgcca
    16741 atagtaaaga aaacattgcc agagatctga acctggctaa aaatgcagga ttcacagata
    16801 ttgtagttga tattcgaccc acaaacggag atatattgta tcagtcatct gtcgaaggtt
    16861 ccaaacaagt aacatggctt ccggcttggg taaacggtgt ttattccaaa gtagagcgta
    16921 cagaaacatg ggattatctt caggcattta ttgataaagg acacgaactc gggctcaaag
    16981 ttcatgccgg atttaacaca atggtaggtg gtaatggtaa cggattgggc aatcaaggtc
    17041 tcctttacag agataacaca aaaaaatcgt gggctacttc tgaaaatttg acttctggta
    17101 taaccaatac gatggacaat ggcgatggaa ctaaattttt caatccggct aatgacgaag
    17161 ttcaaaatta catctgtaac cttttgaaag atctggcaaa atatgattta gatgggattt
    17221 tcttggatag aggacgtttt gacgggatta aatctgattt ttcagacatt acccgccaaa
    17281 aatttcaaac ttatatcggt gggtcaacga taactaattt ccctgaagat attcttccta
    17341 aaggagctac aatgcagacc atatcttcaa tgagcaatta cccggtatac tttaaacaat
    17401 ggctggaatt cagagcaaaa ataatgtatg attttatgga aaaagcaaaa aacgccatta
    17461 aatctgtaaa ttcaaaaata aaatttgggg tatatgtagg tggctggtat tcttcctact
    17521 atgaagttgg tgtgaattgg ggtagtaaaa aatacaatac gtcatcttac tacagtaaat
    17581 gggctacggc aaattaccaa aattatggtt atgccgggtt aatggaacaa atgttgattg
    17641 gagcttatgc ctcaccactt agagtatacg gcacaactga atggaccatg cagggatttt
    17701 gcaaattggc aaaagaaaaa acaaaaggag attgccccat ggtagccgga ggacccgatg
    17761 ttggcaattg ggattcaaat aatactgcaa cacaagaaga agaaaaccag gcaatagtca
    17821 attctgtaaa agcttgcatg gatgaatgtg atggatattt tttattcgat atgattcatt
    17881 taaaaatggc taatcaatgg atttatgcaa aagaaggtat tgataaaacg ttaacagcaa
    17941 attaaacatg attaaatcca aattttctcc tataatatgt atagtgagct tactttgtgt
    18001 tatacttccg gaacacagta aggggcaata tatttcaata tcgggaaaag ttatctgcaa
    18061 tgggaaaggc gttgaaagtg tagtagttac cgatgggaat acatgtgtaa cgaccgataa
    18121 aaagggatta tatactattc aaacttcaaa aagcactgat tttgtctatc tttctactcc
    18181 gagtggttat cttccgcatg atagtttgat gatacctcat ttttataaaa ggttgaattt
    18241 taataaaagc aaaatttata attttgaatt aaagaaaaat cccaaaaatg attacaatca
    18301 cacattatta gttcatactg accctcaatt ctataaaaca ggtaacttta atcattatca
    18361 gaaagtaatt gaagattgtc aagaaacaat caataaatac aaaaatgtgg atgtattcgg
    18421 aattgattgt ggagacctgg taggcgataa accagaattc tatcctacat atatagaata
    18481 cctgagcaag acagaaatcc ctttttaccg cgtattaggc aatcatgata tggattatag
    18541 cagacgttct gatgaaacat ctaccacaag ttataataag atattcggac ctgcctatta
    18601 ttcttttaac aaaggaaaaa atcactacat tattcttgat gatatttttt atatcggtcg
    18661 tgactatttt tatatgggct atgtcagcga aaagatttta aattggttaa aggaagatct
    18721 ttcctatgtc ccgaaaggtt caactgtttt tgtgtctatg cacatacctg cacgattaaa
    18781 taacaaatca gagtttttta aatatgatgg atattctatc gcagaacaaa ctgtaaatgt
    18841 ttcagcttta tttcaagtat tggaaccata caatgttcat attctgacag gacatatgca
    18901 ttacaaccga aatattatcc actcctcaac attgtacgaa cataatctgg gagctatttg
    18961 cggaacttgg tggcaaggtg attattgttt agacggatcg ccaatcggat acggcgttta
    19021 cgagattacc ggagaacagg ttaattggta cttcaaaagc gtgggatatc ctcgctcatg
    19081 tcaaatgaga gcatatccgg caggcagcaa caccgaattt ccagacgaca ttattgtaaa
    19141 cgtatggaac tgggataaaa actggaaa
//
LOCUS       DAOPYB010000041        18737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3089_length_18737_cov_10.179638, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000041 DAOPYB010000000
VERSION     DAOPYB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18737
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3089_length_18737_cov_10.179638"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKH58_04950"
     CDS             complement(<1..280)
                     /locus_tag="PKH58_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease domain-containing protein 2"
                     /protein_id="HNX88407.1"
                     /translation="MLTPKITKEAVNALPIAEFRGEILIVDELLKIKPALEYLRQQKF
                     VGLDTETKPSFQRGTTHKVSLIQISTLDKCFLFRLNKIGFPEELLRFLA"
     gene            complement(289..894)
                     /locus_tag="PKH58_04955"
     CDS             complement(289..894)
                     /locus_tag="PKH58_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HNX88408.1"
                     /translation="MTEQKFIYSKNVIEFVTVAAETCLFLENSADFGKIEFVAKATKI
                     LPLLYLKTTMVDISGLDNDEGIEHFVTEEDYIFVKEQVAELLGPDDSFLEVFDPNMPF
                     SDTPIAAFISENLADIYQELKDFAANYQIGEDEIMETSLQTCIEAFAEHWGQKLLNAL
                     RALHSLQYSDTFGTDEDNTDLDPDDYRKLDRDSFLRFQTED"
     gene            complement(933..1913)
                     /locus_tag="PKH58_04960"
     CDS             complement(933..1913)
                     /locus_tag="PKH58_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX88409.1"
                     /translation="MSFYSKHKYQLKTIRRKTVLLLLFPPLLFTILLFRILPVKWVHA
                     FAGFGGMLFYKFGKNSRDRALKNLRMIYSKELTENEIVCMAKSSLTESIKAFFDYMAY
                     SHVKDRKKYFKLVEVQGEEHLRAAYNRGKGVICLIPHLSSWEFAAITPPMLGYETSAA
                     SKAMKMELLEKLMVKFRQRRGMKNITREGSYQALVDVLKRGECLIIMTDQDTKVKGVF
                     VDFMGKTAYTPLGASRLLADTEAALVPMVMTRRENGKYRFIIYPELPTVKTINPSEDL
                     ITNTINQNKVIEDMIRKNPAQWVWMHQRWKTTPESLERYLKARALEKTRK"
     gene            2003..2251
                     /locus_tag="PKH58_04965"
     CDS             2003..2251
                     /locus_tag="PKH58_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88410.1"
                     /translation="MEENTEKLVVAKYYDSVLNAELDMEVLKNSNIESTVTDDMMVGI
                     FPLFDEKERGISLLVFEKDLDVALQILDDYHCSTPVNE"
     gene            complement(2252..2644)
                     /locus_tag="PKH58_04970"
     CDS             complement(2252..2644)
                     /locus_tag="PKH58_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HNX88411.1"
                     /translation="MTNGNKEGYTCRKFSVPTKRFCQTMDLRNNPELIAEYVKRHSEH
                     EIWKEIPAGIREVGILEMEIYLLGTRLFMILETPVDFSWDAAFAKLATLPRQAEWEDY
                     MSVFQLAEKGASSDEKWQMMERIFYLYD"
     gene            complement(2675..3118)
                     /gene="folK"
                     /locus_tag="PKH58_04975"
                     /pseudo
     CDS             complement(2675..3118)
                     /gene="folK"
                     /locus_tag="PKH58_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
     gene            complement(3229..3684)
                     /locus_tag="PKH58_04980"
     CDS             complement(3229..3684)
                     /locus_tag="PKH58_04980"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX88412.1"
                     /translation="MKKIFGVILMTAVIVAGTTSCKSKQKVTEISGANVEATAKTNTQ
                     GTAAATTTTTEEVEKTRNEKFSLAEGETNSAAFNNKYHVVVGSFSIQQNAKNLQRTLN
                     SEGNSAIVVVNEQGMYRVLIASFNEYSQARERINQINTRFPDAWVLVQK"
     gene            3878..4453
                     /locus_tag="PKH58_04985"
     CDS             3878..4453
                     /locus_tag="PKH58_04985"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNX88413.1"
                     /translation="MTFTLPELPYAHNALEPVISEETINYHYGKHHQTYVTNLNGLVP
                     GTEFENADLETIVKKSSGPIFNNAAQIWNHDFYFLSLTPVKGTAPSENLLKAIEAKFG
                     SLDNFKTEFNKAAVTVFGSGWAWLVKTAEGGVDIVKESNAGNPMTSGLTPLLTFDVWE
                     HAYYIDYRNRRADYVAALWELVDWDVVSARF"
     gene            4676..4897
                     /locus_tag="PKH58_04990"
     CDS             4676..4897
                     /locus_tag="PKH58_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88414.1"
                     /translation="MLATVLITTFIVLISVFLLSFRILLVKGGKFPNIHIGGSKALRK
                     RGVGCATTQDRQAQKITGLNITELIKDIE"
     gene            4915..5523
                     /locus_tag="PKH58_04995"
     CDS             4915..5523
                     /locus_tag="PKH58_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX88415.1"
                     /translation="MKNLSLILNGVLLVAVIVLFFLVLGRGTEKVAGPVTKTGNSISK
                     QTLPIAYVNIDSLLLNYTFAKNANEALIKKQEDSRLTINSRARQLQVEMNEFQRKLEN
                     NAFLSRERAEAAQSEIVKKQKDLQDMDGRLSQQLMEQQQKMSEQLRDTINAFMKDFNK
                     DGKYQMIISNTANDNILYAGKAYDITDEVVKQLNGRYSKAKK"
     gene            5768..6013
                     /locus_tag="PKH58_05000"
     CDS             5768..6013
                     /locus_tag="PKH58_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HNX88416.1"
                     /translation="MQTPRIIKNIYVFYRDGFRNMTVGVTLWTLVLIKLFIMFAILRV
                     FFFPNYLNSNYDTNEDKANHVRNELIKNAHPVQPEKN"
     gene            6122..7672
                     /locus_tag="PKH58_05005"
     CDS             6122..7672
                     /locus_tag="PKH58_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNX88417.1"
                     /translation="MDLLDVSLINWSRAQFALTAMYHWLFVPLTLGLGIIQAIMETIY
                     VKTGKEEWKKAAKFWMTVFGINFAIGVATGIILEFQFGTNWSNYSWFVGDIFGAPLAI
                     EGILAFFMEATFISIMFFGWNKVSKGWHLAATWLTIAGATISALWILVANAWMQNPLG
                     MQFNPATVRNEMIDFWSLLSSPVAINKFFHAVTSGWVLGAIFVAGVSAWYLLKKREVD
                     FAKRSIKVASVFGLVATLLVAYTGDGSAYHVAQKQPMKLAVMEGLYKGQQGTPLILMG
                     IVNPAKEKYNDNVEPVLVPLRIPKMLSFLGYRDFNAFVPGIQDIIDGGYTTINDTTKA
                     LSFEQKKMMGQTAINALKQYKEAEKANDTTALATHKAVIDANYKYFGYGYLDKPEDSI
                     PNVPLIFYSFHLMVVLGFFFIALFLVILFYDYKKILEKQKWLLWVTLFTIPLGYLAGE
                     LGWIVAEVGRQPWAIQDVLPVQAAISSIQTSSVQITFFLFLVLFTALLIAEVRIMLKQ
                     IKKGPAED"
     gene            7751..8911
                     /locus_tag="PKH58_05010"
     CDS             7751..8911
                     /locus_tag="PKH58_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNX88418.1"
                     /translation="MITYSFLQHYWWFLISLLAGILSFLLFVQGGQSMIFSLGKTEDQ
                     RRLLVNVLGKKWEYTFTTLVTFGGAFFASFPLFYSTSFGGAYWVWMLILFCFTIQAVS
                     YEYQNKPGRFFSTNTYRSFLFINGLLGTFLLGAAIATFFTGSEFTVNKQNITEFGGQL
                     SPVISQWDNPLHGLEALFDVRNWLFGLAVFFLARTLAVLFFKNRLNNNEIESRLHKVL
                     AVNAVPFLVFFLAFVIWTLFADGFAVKPGTNEVFMEPMKYLNNLLQMPLLLIFFLVGV
                     VLVLFGIIKSFLKDSWRKGIWYAGAGTIITVTTLFLVAGYNGTAYYPSTSDLQSSLTI
                     FNSSSSFFTLKVMSLVSLLIPFIAVYIFFAWRALESTHTSIEEINEKDSHAY"
     gene            complement(9023..9685)
                     /locus_tag="PKH58_05015"
     CDS             complement(9023..9685)
                     /locus_tag="PKH58_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX88419.1"
                     /translation="MDDTTNSHCPLFRNMSQEEHDDFLERNVKDVVEFKKGETVVRQG
                     DVINYMYLLIQGVVRTQMITQEGNTLEIELLEAVTPLAPAFIYADNNQFPVDVIAMES
                     CKFLRISKSDWLNEMVNNEKLLMNFLTLNSNMTVFLTHKLQMISLKSLKYKLAIFLLE
                     KTTSEKNYFILNRSRTQLAEYFGVQRPSLARTLKELEDEGIISTEGRVVTVLNRNKLS
                     GI"
     gene            complement(9834..10535)
                     /locus_tag="PKH58_05020"
     CDS             complement(9834..10535)
                     /locus_tag="PKH58_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HNX88420.1"
                     /translation="MLNKYNYIFLDLDDTIWDFHANAKSSLNDIYHDQKLYEHYPDFE
                     KFYHIYSKRNLELWSQYGQGLITKEFLIVERFLHLLQKVGINDREMAYNMNTDFLENL
                     STKTILMPYAKELLDYCSERKLPMTIISNGFTEVQYRKLRNSGIEGYFAYVVLSEAAG
                     ALKPSPEIFEYALKLNNARKEEALMIGDSFDADILGAINAGIDAVYLNNTGTENETTD
                     NILEIKSLKELIGKI"
     gene            complement(10656..11531)
                     /locus_tag="PKH58_05025"
     CDS             complement(10656..11531)
                     /locus_tag="PKH58_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88421.1"
                     /translation="MKSYIFYIVAALFLFSSCGIKSGREVDQLKAQNDSLMNSKQELE
                     KEVNEYFSAMNEVQENIEKIKNAQNVISVEPLSENTPEDVRTKVVEDMNYINDMIKTN
                     QEELDRLRTRLKRSSFKLSDVEKTLASLTKQLNEESAKVAALQAQIQQKDSVISTLGT
                     KVEELGKNVENLNTQNQEKDTKIQEQDATIHSAWYAIGSRKELKENKIVSSEGIFSSK
                     KVLQSDFNKNYFVKVDARNTKKIPLYSTSKATILTSHPKTSYTLEKENDNYVLVIVNP
                     SEFWSVSKYLVIEVD"
     gene            complement(11639..12310)
                     /gene="rsmI"
                     /locus_tag="PKH58_05030"
     CDS             complement(11639..12310)
                     /gene="rsmI"
                     /locus_tag="PKH58_05030"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNX88422.1"
                     /translation="MKLYVVPTPIGNLEDMTYRAVKVLGEVDLILAEDTRTSGFLLKH
                     YDIKTPMHSHHKFNEHKTVQNVVERIKAGQKVALISDAGTPAISDPGFLLVRACVENE
                     VEVECLPGATAFVPALVASGLPNDRFCFEGFLPQKKGRQTKLNSLVAETRTMIFYESP
                     FRVVKTLTQLAEVFGADRKASVSREISKIYEQTVRGTLAELVEHFTTNNPKGEFVIIV
                     AGFEE"
     gene            complement(12489..13337)
                     /locus_tag="PKH58_05035"
     CDS             complement(12489..13337)
                     /locus_tag="PKH58_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88423.1"
                     /translation="MNKDMIVLLLKRDIQELNLLTEGFEKLTEFPAPLLLLATQKAEN
                     ILKSLSQLDGAQNYTELGEIDYMPFEDERKPVSEKKNFNAPIHQDEETGNIAAETKPV
                     TEDIITPEETPEIIMTDESDATRYTAQEEHEAPHQEVVETVEINTFTTDTATDDTSEI
                     TLQDNPEIITKEVKPEHTEPIIIANKATILVDELMADHNESLGETIANHKIEDIRQAI
                     NIGDRFRFQRELFNGNGEVMNKTIAYLNQLQKFEEAHSFLKAKFNWTADNNHAEDFLQ
                     IVKRRY"
     gene            13493..14074
                     /locus_tag="PKH58_05040"
     CDS             13493..14074
                     /locus_tag="PKH58_05040"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNX88424.1"
                     /translation="MIYSEKNHPNQVKKGSIEVICGSMFSGKTEELIRRLKRAKFAKQ
                     RVEIFKPKIDTRYSEEDVVSHDRTAIRSTPVESSGSILLMSSEVDVIGIDEAQFFDDG
                     LVEVCKQLADNGIRVIVAGLDMDFRCVPFGPMPALMAVADDVFKVHAICVRCGALAYV
                     SHRLVAGDKQVLLGEMTEYEPICRDCYNKVKKD"
     gene            complement(14713..16437)
                     /gene="recJ"
                     /locus_tag="PKH58_05045"
     CDS             complement(14713..16437)
                     /gene="recJ"
                     /locus_tag="PKH58_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNX88425.1"
                     /translation="MINKWIYSDLSEEQKVIKNNLANELSISPILAQLLVQRDINTYD
                     DARSFFRPDLGDLHDPFLMKDMDKAVERLTKAMQKNEKILIYGDYDVDGTTSVSLVYK
                     FLIQFYSNLEFYIPDRYNEGYGISVKGINYAAENDFKLVIALDCGIKAVEKVKYANSL
                     GVDFIICDHHNPDEVLPPAIAVLDAKREDCNYPYKHLSGCGVGFKLMQAFATTNGIDF
                     QNLIPLLDLVALSIASDIVPITGENRILAFFGLKQINTNPSTGIKAILEVCGLKDKEI
                     SISDIVFKIGPRINASGRMKLATEAVELLVSRDYAFAYKRSSTINVYNNDRKDLDKNI
                     TDEALSLIQSDECYSNRKSIVVYKPDWHKGVIGIVASRLSEEFYKPTIVLTKSNGFAS
                     GSARSVSGFDLYKAIETCRDLLENFGGHMYAAGLTMKEENVDEFTRRFEEYVAENILE
                     DQTYPQIDIDAVLEFKDITPKFFRVLKQFGPFGPGNMKPIFESKNVYDYGSSRLVGKE
                     QEHLKLELIDSSSENVMSGIAFRMYEYNDHLKALNPLDICYTLEENTFNGNTNIQLMI
                     RDIRKVEK"
     gene            16754..17065
                     /locus_tag="PKH58_05050"
     CDS             16754..17065
                     /locus_tag="PKH58_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX88426.1"
                     /translation="MDNKAENIEKLTTFAKAMGHPTRMEILAFLLEQNTCYFGDIHDV
                     FPNAKATISQHLKELKNAGLIQGEIEGPKVKYCINKENWELARLLFADFFKSGVKTKC
                     C"
     gene            17131..17370
                     /locus_tag="PKH58_05055"
     CDS             17131..17370
                     /locus_tag="PKH58_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX88427.1"
                     /translation="MKIEVLGTGCPKCKSLEKAVRDVVAQNNIDATITKVEDIMEIMK
                     FNVLTTPALVVDGKVVVKGRVPSNDELKQILTNQI"
     gene            17376..17804
                     /locus_tag="PKH58_05060"
     CDS             17376..17804
                     /locus_tag="PKH58_05060"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNX88428.1"
                     /translation="MKKFISLSLVMLFLVSFFWVNSLAADKKTKTAVSKTAKVEVYYF
                     HFTRRCVTCQAVETESQKAIAALYPAQYKSGKIIFKSLNLDEDNSKALAEKCKAEGQQ
                     LLIISGNKRFDITEQGFMYARSNPDKLKQELKTTIDPLIK"
     gene            17812..18513
                     /locus_tag="PKH58_05065"
     CDS             17812..18513
                     /locus_tag="PKH58_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNX88429.1"
                     /translation="MDFLQGILDNSQYAFITAFVLGLMTAISPCPLATNISAIGFISR
                     DIENRQKVFINGFIYTLGRAFSYTLLAVVIFFGASKLNISMLFQGWGEKLLGPALILI
                     GLFMLDVIKLKFPGLSGLTEKLGEKSKGSYWSTFFLGVVFALAFCPYSGVLYFAMLIP
                     MTVASASGLYLPVVFAVATGLPVILFAWLLAYAVGNVGKLYNKIKTFELWFRRVVSVI
                     FIGVGIYYVVIFYFI"
BASE COUNT         5082 a         3729 c         3974 g         5952 t
ORIGIN      
        1 cggccagaaa tcgcagtaat tcttccggaa agccaatttt attcaatctg aaaaggaagc
       61 acttgtccag ggttgaaatc tgtatcaacg aaaccttatg tgtggttcca cgctgaaacg
      121 aaggtttggt ttcggtgtcc agaccaacaa atttctgttg tctgagatat tccagcgccg
      181 gttttatttt gagaagttca tcgacgataa gaatctctcc cctgaattcg gcaatgggta
      241 acgcgttgac tgcttctttt gttattttgg gtgtaagcat ttttgtgttt aatcttctgt
      301 ttgaaatctc agaaaactat cccgatcaag ttttctgtaa tcatccggat ctaagtcagt
      361 gttgtcttca tctgttccga aagtatcgct gtactgcaga gagtgaagag cgcgcaaagc
      421 attcagcagt ttctgtcccc agtgttcggc aaacgcttcg atacaagtct gcagtgaggt
      481 ttccatgatt tcgtcttcac caatctgata gttggcagca aaatctttca gttcctgata
      541 aatatctgcc agattttcgg aaatgaatgc ggctatcggg gtatcgctga aaggcatatt
      601 cggatcgaat acttccagaa aactatcgtc aggtcccagc aattcagcga cctgttcctt
      661 tacaaaaata tagtcttctt ccgtaacgaa atgttcgata ccctcgtcat tatcgagtcc
      721 cgaaatatca accatagtgg ttttcagata aagcagaggc agtatttttg ttgctttggc
      781 tacgaattca atttttccga aatcggcaga gttttccaga aaaaggcatg tctccgctgc
      841 cacagttacg aattcaatga cgttttttga atagataaat ttttgctcag tcatattcaa
      901 ttttttaagt ttacaaaagt agcaaaaatt ctctactttc tcgttttttc aagggctctt
      961 gctttcaggt atcgttcgag cgattcggga gtagttttcc accgctgatg catccagacc
     1021 cactgagcgg gatttttacg tatcatatct tcaatcactt tgttctgatt gatggtattg
     1081 gttatcaggt cttctgaagg attgatggtt ttcactgtcg gaagttcggg gtaaataata
     1141 aaccggtact ttccgttttc gcgacgggtc atcaccatgg gaacaagagc tgcttccgta
     1201 tcggccagca agcgcgaagc tcctaaaggt gtgtaagctg tttttcccat aaagtcaaca
     1261 aaaacgcctt tcacttttgt gtcctggtcg gtcatgatga tcaggcattc gccgcgtttc
     1321 agcacatcga caagcgcttg gtatgaacct tcgcgggtaa tatttttcat tcctctccgc
     1381 tggcgaaatt ttaccatcag cttttcgagc agttccattt tcatggcttt actggctgcg
     1441 gatgtttcat aacccagcat gggaggtgta attgctgcaa attcccagga actcaagtga
     1501 ggtatcagac aaataactcc ctttcctcta ttgtacgcag cccgtaaatg ttcttctccc
     1561 tgaacctcta ccagcttgaa gtattttttc cggtctttaa cgtgcgagta tgccatgtag
     1621 tcgaaaaaag cctttattga ttcggttagt gatgattttg ccatgcacac aatttcgttc
     1681 tcggtcagtt ctttgctgta aatcattctg aggtttttca gtgcgcggtc tcgggagttt
     1741 ttaccgaatt tataaaacaa cattccccca aaaccggcaa aagcatggac ccatttgact
     1801 ggtaagattc tgaacaggag tatggtgaaa agcagtggag ggaaaagtaa caacagcacc
     1861 gttttacgac ggatagtctt tagttggtat ttatgtttcg aatagaaaga catttgttaa
     1921 aaatataaaa caataatgac ggaatgaatg ttataatagc aagatcgact atatttatcc
     1981 aattaataaa aaacagtaag caatggaaga aaatacagaa aaacttgttg ttgcgaaata
     2041 ttacgattca gtgctgaacg ctgaacttga tatggaagtg ctaaagaaca gcaacattga
     2101 gagcacggtt acggacgaca tgatggtcgg gatttttccg cttttcgacg aaaaagagcg
     2161 gggtatcagt ctgctggtgt tcgaaaaaga tctggacgta gcgcttcaga tacttgacga
     2221 ttaccactgt tcgacccccg tgaacgagta atcagtcgta caggtaaaaa atccgctcca
     2281 tcatctgcca tttttcatcg gaacttgctc ctttttcagc aagctggaaa acagacatat
     2341 aatcttccca ttcagcctga cggggaagtg ttgccaattt ggcaaatgct gcatcccaac
     2401 tgaaatcaac gggtgtttcc agaatcatga aaagtcttgt gccgagcagg taaatttcca
     2461 tttccagaat accaacttca cggatacccg cgggtatttc tttccatatc tcatgctcgc
     2521 tgtgacgttt cacatattca gcgatgagtt ccggattgtt tctcaaatcc atggtctggc
     2581 aaaatctttt ggtaggaacg ctgaatttcc tgcaggtgta accttccttg ttgccgttcg
     2641 tcatttttac atcagattta ttaagtttat ttttcagttc aatgattgac ttattgagta
     2701 ccggatgtac cagatccgga gcaatttcgg ccaatggcgt cagcacgaag tctctgaggt
     2761 gaagcatcgg atgtggcaat tccaaccccg gttgatctat tattctgttt tcgtagaata
     2821 aaatgtcgat atcgatgata cgatccgaat aacctgtatc cgatttggtg cttctgccca
     2881 tctttcgttc tatctctttg gtcttttcga gcaacgaaaa cgggtcaagt ttagtatcga
     2941 taagaatgac agcattcaga aaatcgttgt cggagttgaa tccccagggt ttcgattggt
     3001 aaaatgatga aacatttgct attgcaccgg cttcacagcc aataatacgg atagcgtgat
     3061 taagattttg ggctttgtcg cccagatttg ttcccaagcc aataaatacc agcgacattt
     3121 tttctttttg atgcaaatgt agtgattttt gaatatgcaa ccgtttaaaa acaaaacgac
     3181 tttgcagttg acaaagccgt ttttatttat gtttggtgtt cagctgtatt atttttgcac
     3241 cagcacccat gcgtcgggga agcgggtgtt gatctgattg atgcgttcac gagcctgaga
     3301 gtattcgttg aacgatgcaa tgagaacgcg gtacatgcct tgttcgttga caaccacgat
     3361 ggcgctgttg ccttcgctgt tcagcgtacg ttgcagattt ttcgcatttt gctgaataga
     3421 aaaactgcca actacaacat ggtatttatt gttgaaagca gcagagttgg tttcaccttc
     3481 agccagactg aatttttcat tacgggtttt ttcaacttct tcggtagtgg tggttgttgc
     3541 agcggcagtt ccctgagtat tggtctttgc agttgcttca acattggctc ccgaaatttc
     3601 agtaactttt tgttttgatt tacatgatgt agtgccggcg acaatgacag cagtcatcag
     3661 gattactcca aaaatctttt tcattttata aaaatttatg tttgtaaaaa tttgactatt
     3721 tttgtattgc aaagatgcta ataaaaatga agttaagcaa ttatttagtt cgttttttac
     3781 attatttaca attttgcgat aaacaaattg aatactattt tgtttttcat ctgttactga
     3841 aaaatgatat tattaacact ataaaaatac aaagattatg acatttacat tgcccgaatt
     3901 gccttatgcg cacaatgcgc tcgaaccagt tatcagcgaa gaaacgatta actatcatta
     3961 cggaaaacat catcaaactt atgtgactaa tctgaacgga ctggtacccg gaactgaatt
     4021 tgagaatgca gaccttgaaa ccatagttaa gaaatcaagt ggacctattt ttaataatgc
     4081 agctcagata tggaatcatg atttttattt cctttcgctg acgcctgtaa agggaacagc
     4141 accatccgaa aatttattaa aagcaattga agcaaaattc ggatcgcttg acaatttcaa
     4201 aactgaattt aataaagcag ctgtaaccgt attcggatcg ggatgggctt ggctcgtaaa
     4261 aactgctgaa ggaggagttg atatcgtaaa ggaaagtaat gccggaaatc cgatgaccag
     4321 cggattaaca ccactgttga ctttcgatgt ctgggaacat gcttattaca ttgattaccg
     4381 taatcgtcgt gctgattatg ttgctgcgtt atgggaactc gttgactggg atgtggtatc
     4441 agcaaggttt taacaacaac ttattatcag gaaagaagcc ggaaagttac aaacactttt
     4501 tcggcttttt tcatttaatt aatgtaaaac ccgacggaaa tccccaaaaa aacaaatgaa
     4561 agttaaaata aaatctgaaa cattcaattt tctaaaaaat attcgaagct attactgaaa
     4621 tgtatcaaaa aaactatctt tgcataaatt ctttgagttt ttatacaata tgaatatcct
     4681 tgcaactgta ttaattacaa catttattgt gctgatttcg gtttttctgc tgagtttcag
     4741 aatacttttg gtgaagggcg gaaaatttcc caacattcat attggtggta gcaaggcttt
     4801 acgcaagcgc ggtgtgggtt gtgcaacgac gcaagaccgt caggcacaaa aaataaccgg
     4861 attaaatatt actgaattga ttaaagatat agaataaaat aattaaaaat gaatatgaaa
     4921 aatctatccc tcattctgaa tggtgtttta ttggtagcag ttattgtcct gttttttctg
     4981 gtacttggca gaggaactga aaaagttgcc ggccctgtga cgaaaaccgg aaattcgatc
     5041 agcaaacaga ctttacccat tgcctatgta aacattgact cattgctact caattacaca
     5101 tttgcaaaaa acgcaaatga agcattgatc aaaaaacagg aggattcaag acttactata
     5161 aactcacgcg cacgccagtt gcaggtagaa atgaatgaat ttcagcgtaa actcgaaaac
     5221 aatgcttttc tgagccgcga gagagccgaa gctgcacaat ctgagattgt gaagaaacag
     5281 aaggatctgc aggatatgga tggaagatta tcgcaacaac tgatggaaca gcagcagaaa
     5341 atgagtgaac aactgcgcga taccatcaat gctttcatga aagattttaa taaagatgga
     5401 aaatatcaga tgattatcag caataccgcc aatgataata tcctctatgc gggaaaggct
     5461 tatgatataa ctgatgaagt agtcaaacaa ctcaacggaa gatattcgaa agccaaaaag
     5521 taactcaaca caaaatcatt caataagaaa ataaacggaa tcatacttat tatagtatgg
     5581 ttcttttttt ttacacttct tataactgcg aacggtaaaa ttatacgttt gcaaagccca
     5641 acttcaaaac acattcagaa gggattattt tcaggtaaac ataaaaaatt attataaaaa
     5701 aatcactttt ttaaaatata tgtgtatatt tgtttttgtg ttatcaacaa ttaccagaat
     5761 cttttttatg caaacgccac gaattattaa aaatatctat gttttttatc gcgacggttt
     5821 caggaacatg accgtaggag tgactctctg gacattagtg ctgatcaaat tattcatcat
     5881 gtttgctatc ctccgggttt tctttttccc aaactatctc aattccaatt atgataccaa
     5941 tgaggataaa gccaatcatg tcagaaacga attaattaaa aatgcgcatc ctgtacagcc
     6001 tgagaaaaat taagtattta atgtctggaa cgtatgattg attggctgga atggactggt
     6061 actgtttatg ttaaaaatga aaaattatcc ttatgtttaa taaatttaat acattatttt
     6121 tatggattta cttgatgtgt ctttaattaa ctggtcgcgg gcgcagtttg ccctgacagc
     6181 gatgtaccac tggctttttg tgccgctaac tctcgggctt ggtatcattc aggctatcat
     6241 ggaaactatc tatgtgaaaa ccggtaagga ggaatggaaa aaagctgcta aattctggat
     6301 gaccgtattc ggcatcaact ttgccatcgg ggttgcaact ggtattatac ttgagttcca
     6361 gtttgggact aactggtcca attacagctg gtttgtgggt gatatttttg gtgcaccact
     6421 ggccatcgag ggtatcctgg ctttctttat ggaagctacg ttcatttcta tcatgttttt
     6481 cggctggaac aaggtgagta aaggctggca tctggcagca acctggctga ctattgccgg
     6541 tgcaaccatc tctgcgctct ggattttggt agccaatgcc tggatgcaaa atccactggg
     6601 tatgcaattt aatccggcaa cagtgcggaa cgagatgatt gatttttgga gtttactgag
     6661 ttcgcctgtt gctatcaata aattttttca tgccgttact tcaggctggg ttcttggggc
     6721 tattttcgta gccggtgtca gtgcgtggta tttgcttaaa aaacgcgaag ttgattttgc
     6781 aaaacgcagt atcaaagttg cgtccgtttt cggtttagtg gccacactgc tggtagccta
     6841 taccggtgat ggctctgctt atcatgtagc gcagaaacaa cccatgaaac tggctgtgat
     6901 ggaaggcttg tataaaggtc agcagggtac tccccttatt ttaatgggga tcgtaaatcc
     6961 ggctaaagaa aaatacaacg ataacgttga gcctgtactg gttcctctcc gaattccaaa
     7021 gatgctttca ttccttggtt accgcgattt taatgctttc gttcccggta ttcaggatat
     7081 catcgacggt ggttacacaa cgataaatga tacgacaaaa gcattgtcgt tcgaacagaa
     7141 aaaaatgatg ggtcagacgg caatcaatgc gctgaaacag tacaaagaag ctgaaaaagc
     7201 gaacgacact accgcgctgg caacccacaa agcggtgatt gacgctaatt acaaatactt
     7261 cgggtacggt tatctggata aacccgaaga ctctataccc aatgtgccgt taatattcta
     7321 ttctttccat cttatggtgg tgttaggctt cttctttatc gctttgtttc tggtcatttt
     7381 gttctacgat tacaagaaaa tacttgaaaa acagaaatgg ttattgtggg ttactttatt
     7441 caccatacca ctgggttatc tggcaggcga actgggttgg attgtagctg aagtgggacg
     7501 tcagccctgg gctattcagg atgtattacc cgtacaggct gccatttcca gtattcagac
     7561 atcatccgtg caaatcactt tcttcctgtt cctcgtattg tttacggctc ttttgattgc
     7621 cgaggtacgt attatgctca agcaaatcaa aaaaggtccg gctgaagatt aatcggattg
     7681 ccggttttat aatatgattt ttccataaaa cagtagaata aattttcatt aaaatcaaat
     7741 aaaatacact atgataacat attcatttct acaacattac tggtggttcc taatctcact
     7801 gctcgcaggt attctttcct tcctgctttt tgtgcagggt ggtcagtcta tgattttctc
     7861 actgggaaaa acggaggatc aacgccgttt gctggtcaac gtgctgggaa aaaaatggga
     7921 atacaccttc accaccctgg tcactttcgg tggcgcgttt tttgcatctt ttccgttgtt
     7981 ctattccacc agtttcggtg gcgcctactg ggtgtggatg ctgatacttt tctgttttac
     8041 tattcaggca gtatcctacg agtatcagaa taaacccgga cgttttttca gcaccaatac
     8101 ctatcgcagt tttctgttta tcaatggatt gcttgggact tttctgttag gcgctgctat
     8161 tgcgacgttc tttacaggct ccgaatttac tgtcaacaaa caaaacatca ctgaatttgg
     8221 cggacagcta tcgccggtaa tcagccagtg ggataatccg ctgcatggac tggaggcatt
     8281 gtttgacgtt cgcaactggt tgttcggtct ggcggtattc tttcttgcac gaacgctggc
     8341 tgttcttttc ttcaaaaacc gtttgaataa taatgaaatc gaaagccgtc tgcacaaagt
     8401 cctcgctgtg aatgctgtac cttttttggt tttcttcctc gcttttgtaa tctggacact
     8461 ttttgctgac ggatttgctg tgaaaccggg tacaaacgaa gtatttatgg agcctatgaa
     8521 ataccttaat aatctactcc agatgccgct gttacttatt tttttcctgg ttggagtagt
     8581 tctggtgttg ttcggaatta taaaatcatt tctcaaagac agctggcgca aggggatctg
     8641 gtatgccgga gcaggcacaa ttattactgt taccaccttg tttttggtcg caggttataa
     8701 cggaacagct tactatcctt ctacttccga tctgcagagt tcgctgacaa ttttcaattc
     8761 gtcctccagt tttttcaccc tgaaggtgat gtcgctggta tcgttgctca taccattcat
     8821 tgctgtgtat attttctttg catggcgtgc acttgaaagt acacacacca gtatagagga
     8881 aattaacgaa aaagactcac acgcttacta aaaaagcaat ataattaact gaaacaatga
     8941 ccgatttgct tccagaccgg tcattgtttt tttttatttc tgccttaata atttgtatgt
     9001 taatgcatgt tttactaatt tttcagatgc cggaaagttt attcctgttc aggactgtaa
     9061 ccacacgccc ttcagtactg ataatacctt catcttccag ttccttaagc gtgcgggcaa
     9121 gtgaaggacg ctgaacaccg aagtactcgg caagttgagt ccgcgaacgg ttcaggatga
     9181 aatagttttt ttcggaagtt gtcttttcca gtaaaaagat tgccagcttg tatttcaggc
     9241 ttttgagcga gatcatctga agtttatgcg tcagaaacac tgtcatgttt gaattcaacg
     9301 tgaggaaatt catcagaagt ttttcgttgt tgaccatttc attcagccag tcggattttg
     9361 atatcctcag aaacttacac gattccatgg cgataacatc aaccgggaat tgattgttgt
     9421 ctgcatagat gaaagccgga gcgagggggg tgactgcttc aagcaattct atttccagtg
     9481 tattaccttc ctgggttatc atttgagtcc tgacaactcc ctgtatcagc aggtacatat
     9541 aatttataac atcgccctgt cgaaccactg tttcaccctt tttgaattcc acaacgtcct
     9601 ttacgttccg ttccagaaag tcatcgtgct cttcctgact catatttctg aaaagcggac
     9661 agtgactatt tgttgtatcg tccataagat tcaatgtttt tttctagtga aaataatcag
     9721 tgtaattatt aaataaaatc atctcaaagt ggcttgcaat agggcttatt tgtttatgtt
     9781 caatcgaaaa tgatgagcaa gcaatgatta tgaaatatta tcggaagacc ggatcaaatt
     9841 tttccaataa gttctttgag gcttttaatt tccaggatat tgtcagtagt ttcattttcc
     9901 gtgcccgtgt tgttgaggta cacggcatcg atgcccgcgt tgatggctcc caggatatca
     9961 gcgtcgaagc tatcacctat catcagcgct tcttctttac gggcgttgtt gagtttcagg
    10021 gcgtattcga atatctccgg actgggtttc agcgctccgg cagcttccga aagcactacg
    10081 taggcaaaat acccttctat gcctgagttg cgcagtttgc ggtattgcac ttcggtgaag
    10141 ccgttggaaa tgatggtcat tggcagcttt ctttccgagc agtaatccag caattctttg
    10201 gcataaggca tgagtatggt cttggttgaa agattttcaa gaaagtcggt gttcatattg
    10261 taagccattt cccggtcatt gatacccact ttctgaagca ggtgcaggaa gcgctccaca
    10321 atcaggaatt cctttgttat cagtccctgg ccgtattgcg accagagttc cagatttctt
    10381 ttggaataga tgtgatagaa tttttcgaag tcggggtagt gttcgtacaa cttttgatcg
    10441 tggtagatat cgttgagcga cgacttggca ttggcatgaa aatcccagat tgtatcgtcg
    10501 aggtcaagga atatgtagtt gtatttgttt agcaagactt gtcgtttcgg gtttcgtgtt
    10561 cagtgtttca agtttcatgt tccgtgtttc tttcagtata caggacttgt aaaagtcccc
    10621 ggtttagttt tctgccgggg acgttgtccc gataatcaat cgacttctat caccaggtat
    10681 ttgcttacac tccagaattc cgaagggttc actatcacca gtacataatt gtcgttttct
    10741 ttctcaagtg tatagctcgt tttagggtga cttgtcagga tggttgcttt cgaagtggaa
    10801 tacagaggta ttttcttcgt gttgcgtgca tccactttca cgaaatagtt tttattgaag
    10861 tcagattgaa gcactttttt tgaactgaaa ataccctccg atgaaacgat tttgttttct
    10921 ttcagttcct tgcgtgaacc gatggcatac caggctgaat gaatagttgc atcctgttcc
    10981 tgtattttcg tatctttttc ctgattttgt gtattgaggt tttccacatt cttgcccagt
    11041 tcttccacct ttgttcccag tgtgctgatg acggagtctt tctgctgtat ctgagcctgg
    11101 agcgcggcta ccttagccga ttcctcgttt agctgttttg taagcgaagc cagtgttttt
    11161 tccacatcag acagtttgaa cgaactcctt ttgaggcgtg tacgcaggcg gtcgagttct
    11221 tcctggttgg tttttatcat gtcgttgatg tagttcatgt cttccaccac tttggtacgc
    11281 acatcttcgg gtgtgttttc gctaagcggt tcgactgaaa ttacattctg tgcgttttta
    11341 attttctcaa tattttcctg tacttcgttc atggccgaga agtattcgtt cacttctttt
    11401 tccagttctt gttttgagtt catcagcgag tcgttctggg ctttcaactg atcaacttca
    11461 cgtccgcttt taattccgca ggaagagaat aaaaacaggg cagcgacgat ataaaaaatg
    11521 taagacttca tctttttatg gtttgttatc tggtaacaga acggatttat tatttgtttg
    11581 tgatgtaagt gttaatttat ggtgataatt gttttattat ctaagcgcaa aggtaacgct
    11641 attcttcaaa ccctgcaaca attataacaa attccccttt gggattgttg gtggtgaagt
    11701 gctcaaccag ttcagccagt gttccccgta ccgtttgttc ataaatcttt gatatctcgc
    11761 gcgatacgga agctttgcgg tctgctccga acacttcggc aagctgtgtc agggtcttga
    11821 ctacccggaa aggcgattcg tagaatatca tggttcttgt ttcggcaacg agcgaattaa
    11881 gttttgtctg acgtcctttt ttctggggca gaaacccttc gaaacagaag cggtcgttgg
    11941 gcagacccga tgcaaccagt gccggtacaa atgcagttgc tcccggcaga cattccactt
    12001 ccacttcatt ctcaacgcag gctctcacca gcagaaaacc cgggtcggaa attgccggag
    12061 tgcccgcgtc ggagatgagc gccacctttt gtccggcttt gatacgttcc accacgtttt
    12121 gcaccgtttt atgttcgttg aatttgtggt gcgaatgcat tggggttttg atgtcgtagt
    12181 gtttaagcag aaaaccgctg gtacgcgtat cttccgctaa aatcaaatcg acctcaccaa
    12241 gtacctttac agcacgatag gtcatatctt ccaggttacc gatgggtgtg ggtactacgt
    12301 ataatttcat gtgtttattt atatgccccc taatttccct aaagcgggac aaggctggtt
    12361 attattcatt tactttttat atccaaataa tttattttgg acatgagtgt ataatgcaaa
    12421 gacaatcgtg tttttgatat catttcagca tttgaaaagt aattctgcca gtcaccaatc
    12481 accggtcact aataccttct tttcacaatc tgtaaaaaat cctctgcatg gttattatct
    12541 gctgtccagt tgaatttagc cttgagaaac gaatgcgctt cctcgaattt ttgcagttgg
    12601 ttgaggtacg cgatggtttt attcatcact tcaccgttgc cgttgaacag ttcacgctgg
    12661 aaacggaagc ggtcgccgat gttgatagcc tgacggatat cctctatttt atggttggct
    12721 attgtttcgc ccagtgattc gttgtggtcg gccatcagtt catctaccag tatggttgct
    12781 ttgttggcaa tgatgattgg ttcggtatgt tccggtttca cttcctttgt tataatctcc
    12841 gggttgtcct gaagggtgat ttctgaagta tcatccgtag cggtatcagt ggtaaaagta
    12901 tttatttcaa cagtttccac cacttcctga tgcggagctt cgtgttcctc ctgtgccgtg
    12961 taccgtgttg catcactttc atcggtcatg ataatttcag gagtttcttc cggtgtgata
    13021 atatcttctg ttaccggttt tgtttcagcc gcgatgtttc cagtttcttc atcctgatga
    13081 atgggagcgt taaagttctt tttctcactt accggttttc tttcgtcttc aaacggcata
    13141 taatcaatct ctcccagttc ggtgtagttt tgcgcgccgt ccagctggct caggcttttc
    13201 agaatgtttt cggctttctg tgttgccagt aaaagcagcg gagcaggaaa ttcggttaat
    13261 ttctcaaatc cttcggtcag caggttcagt tcctgaatat ctcttttcag caagagcaca
    13321 atcatgtctt tgttcatatc tttttcctaa aaaacaggtt ctgtaaaagt atttttttta
    13381 tttttgtaca aattattggt tatttgcagt ccattctgaa aatacgctgt aaagatacta
    13441 attaatgaat tgaaaagaaa acggttaaaa catatttttc aacaaaatac caatgatcta
    13501 ttcggagaaa aaccatccca atcaggtaaa gaaaggcagt atcgaagtaa tctgcggttc
    13561 catgttttcg ggcaaaaccg aggaattaat ccgcaggttg aaacgcgcca agtttgcaaa
    13621 acagcgggtg gaaatcttta agcctaagat tgatacgcgc tattcggagg aagatgttgt
    13681 ttcgcacgac cggactgcga tacgttccac tcctgtagaa tcatcgggaa gcatcctgct
    13741 tatgtccagc gaggtggatg ttatcggcat tgacgaagca cagtttttcg acgacggact
    13801 ggttgaagtt tgcaaacagc ttgcagataa tggcattcgt gtgattgttg ccggtcttga
    13861 tatggatttc cgatgcgttc cctttgggcc tatgcctgcc ctgatggctg ttgccgacga
    13921 tgtttttaag gttcacgcca tttgtgtacg ctgcggcgcg ctggcctatg tttcccatcg
    13981 gttggtggca ggcgacaagc aggtgctgct gggcgagatg accgaatatg aaccaatctg
    14041 ccgggattgt tacaacaaag taaaaaagga ctaaaataaa taataaacgc tgagaataat
    14101 ctcagcgttt attattaaaa ttaattagat aaagcatcaa ctctcctcgg ggtagtctcc
    14161 ggacgttaat atgtgaaaat ctaatttctg tcaggaaaat ctattcccgt tttcattata
    14221 acttactgaa ttaaataatc attcgatttt ccggggctaa tataccgaaa acaaaaatac
    14281 cttgcaagga atttgcaagg cagaacaaaa agttgatgta aataagatac atatctgtat
    14341 tataaacaga cagatagctt tatggaagtt gatattcgaa atgattggtc gaaatgggta
    14401 tttcttcaaa tatcgttaaa ttctatttct taaaatttca gttttcaact ctataaacct
    14461 ccttttattg atgtgtcttc gcaaagtcag gatttaagcc agcgcactgc tttatgtaat
    14521 gccggagcat tacttttagc acgaggcttc ggcaagctca gttcagtacg ggagatattt
    14581 aagcagacga caccgagcct taggggaaat aagtaccctg ctgctacacg ttacaaatgc
    14641 gcagcagcgg ggctctttta tttttgactg gtatttattc tataaacaat agactgtaaa
    14701 ctctcccgaa cactatttct ccactttccg gatgtcccgt atcatcagct ggatgtttgt
    14761 atttccgttg aaagtgtttt cctcgagggt atagcagatg tcgagtgggt tcagggcttt
    14821 caggtggtcg ttgtattcgt acatgcggaa ggcgatgccg ctcatcacat tctccgagct
    14881 gctgtcgatc agttccagtt tgaggtgttc ctgctcttta cctactaggc ggctgctgcc
    14941 gtaatcatac acgtttttcg attcgaagat tggtttcatg tttcccgggc cgaacggacc
    15001 gaattgtttc agtacgcgga aaaatttggg tgtgatgtcc ttgaattcga gcaccgcgtc
    15061 aatatcgatt tgtgggtaag tctggtcttc caggatgttt tcagctacgt attcttcaaa
    15121 ccggcgtgta aattcatcga cattttcttc cttcatggta agtcccgcgg catacatgtg
    15181 tccgccgaaa ttctcaagca ggtcgcgaca tgtctcgatg gctttataaa ggtcaaaacc
    15241 tgatactgaa cgggcggaac ccgatgcaaa gccgttggat tttgtaagca caatggtagg
    15301 cttgtagaat tcctccgaaa ggcgcgaagc tacaatcccg attactccct tgtgccagtc
    15361 gggtttatac accacgatgg atttgcggtt ggaatagcat tcgtccgact gtataagcga
    15421 cagcgcttcg tcggtgatat ttttatccag gtctttgcgg tcgttgttgt aaacattgat
    15481 tgtgctgctt cgtttgtaag cgaaagcata atcgcgcgat acgagcagtt ccactgcctc
    15541 ggtagccagc ttcattcgtc ccgatgcgtt gatacgcggc cctattttga aaacaatatc
    15601 gctgatgctg atctccttgt ctttcagtcc gcatacttcc agaatagctt tgatgccggt
    15661 actcgggttg gtgttgattt gtttgaggcc gaagaaggcg agaatacggt tttcgcccgt
    15721 gatgggtaca atgtctgagg caatgctcag cgctacaagg tccagcaggg ggatgaggtt
    15781 ctgaaagtca atgccgttgg ttgtggcaaa agcctgcatc agtttgaaac ccactccgca
    15841 tcccgacaga tgtttgtagg gataattgca gtcttcgcgt tttgcatcga gtacggcaat
    15901 ggcaggtggc agtacttcgt cgggattatg gtggtcgcag atgataaaat caacgcccag
    15961 cgaattggca tacttcactt tttccactgc cttgataccg caatcgaggg cgataacgag
    16021 cttaaagtcg ttttcggctg cataattaat gcctttgaca gatattccgt agccttcgtt
    16081 gtagcggtcg ggaatataga actcgaggtt ggagtagaac tgaatgagga atttataaac
    16141 cagagatacg gacgtagttc catccacatc ataatcgccg tagataagta tcttttcatt
    16201 cttttgcatc gccttggtca gtctttccac cgctttatcc atatccttca tcaggaaagg
    16261 atcatgtaaa tcacccaggt cgggacggaa aaaactgcgt gcgtcatcgt atgtgttaat
    16321 gtcgcgttgc accagcagct gtgctaatat gggactaata cttaattcat tggcaagatt
    16381 atttttaatt actttttgtt cttcggaaag atccgaataa atccatttat ttatcatttt
    16441 gtgtactatt ttttcaggca agggtgaaat atcgctatat ttcactgtaa aaatcaactc
    16501 cgtaatcatc cccgcgttca aaccccgttt actcaacttt tattcacaga aaaacaactt
    16561 acccagcgaa tcgttcaaat gttttctgta taatctgtaa aaataataca ttttaatgta
    16621 aatgacaaaa ttcaacggta aaattattgt agaattctgc tccgtaattc atttgtaact
    16681 gataagaaaa acttcggaaa aatgtttgat atatgaaata ttgttcgtat ttttgcgaac
    16741 gtataattcg aacatggata ataaagcgga aaatatcgaa aagctgacca ccttcgccaa
    16801 agcgatgggg catccaaccc gtatggaaat tctggcgttc ctgctggagc agaatacgtg
    16861 ttatttcgga gatatccatg atgtttttcc caacgcaaaa gcaaccatct cgcaacatct
    16921 gaaagaattg aaaaacgcgg gactaattca gggcgaaata gaaggaccga aagtgaagta
    16981 ctgtatcaat aaggaaaact gggaactcgc gagacttctt tttgcagact ttttcaagtc
    17041 aggtgtaaaa acaaaatgct gttaacagct tttttttgaa aataatgttc gttaatcgac
    17101 gaacagataa ctatacataa aataaaaaat atgaagattg aagttttagg aaccggctgt
    17161 ccgaagtgca agtcacttga aaaagctgta cgtgatgttg tggctcaaaa caatatcgat
    17221 gctacaatta ccaaggttga agacattatg gagattatga agtttaatgt attgaccact
    17281 ccggcattag tcgttgatgg aaaagtggtg gtgaaaggcc gtgtaccatc caacgatgaa
    17341 ttaaagcaaa ttcttacaaa tcaaatttaa tatctatgaa aaagttcatt tcattatctc
    17401 tggtaatgct ttttttagtc tctttttttt gggtaaatag tcttgccgct gataaaaaaa
    17461 cgaagactgc agtttccaaa acagctaagg tcgaagtcta ttattttcat tttacgcgtc
    17521 gttgcgtaac ttgtcaggct gttgaaactg aatctcagaa agccattgcc gcactttatc
    17581 cggcacagta taaatccggt aaaatcattt tcaaatcact aaatttagac gaagataaca
    17641 gtaaagcttt agctgaaaaa tgcaaggcag aaggacaaca gcttttaatt atcagcggaa
    17701 acaaacgctt cgatattacc gaacaagggt ttatgtacgc ccgaagcaat cctgacaaac
    17761 tgaaacagga attgaaaact accattgatc cgctaatcaa ataatcaata tatggatttt
    17821 ttgcaaggta tattagacaa ttctcaatat gcatttatca cggcatttgt gttgggacta
    17881 atgacagcca ttagtccctg cccactggct acgaatatct cggccattgg ctttattagc
    17941 cgcgatattg agaatagaca aaaagtattc ataaacggct ttatctatac attgggcagg
    18001 gcgttcagtt atactttatt ggcagtcgtt attttctttg gagcaagcaa actgaatata
    18061 tccatgctgt ttcagggctg gggagaaaag ctgttaggtc ctgcgttgat tctaatcgga
    18121 ttgtttatgc tcgatgtaat taagctcaaa ttccccggct tatccggttt aaccgaaaag
    18181 ttaggcgaga aaagcaaagg cagctattgg agtacatttt tcttaggcgt agttttcgca
    18241 ttggcattct gtccttatag tggcgtgttg tatttcgcca tgcttatacc tatgacagta
    18301 gccagcgcaa gcggacttta tctgcctgta gtttttgccg ttgctaccgg attgcccgtt
    18361 attctttttg cgtggctatt ggcttatgca gtgggtaatg ttggaaaact atataataag
    18421 atcaaaacct ttgaactttg gtttcgccgc gttgtatcgg tgatttttat aggtgtgggt
    18481 atttattatg ttgtgatatt ttattttata taaacctgcc cccttaagtc agccccctaa
    18541 ccctcccgta acaagtccgg gacaggctct aaagggggaa ttttaattat cctaattcta
    18601 cttatatagt tcatgatctt acgaaaagta attatattca ggaatggaaa aaactaaaaa
    18661 aactaaactt attctttctt tgtcagcagg attgcctgct ttgatacttc tttatcattt
    18721 cttacaacca ttagtta
//
LOCUS       DAOPYB010000042        18702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3095_length_18702_cov_8.871078, whole genome shotgun sequence.
ACCESSION   DAOPYB010000042 DAOPYB010000000
VERSION     DAOPYB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18702
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3095_length_18702_cov_8.871078"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..>1889)
                     /locus_tag="PKH58_05070"
     CDS             complement(220..>1889)
                     /locus_tag="PKH58_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1961 family protein"
                     /protein_id="HNX88430.1"
                     /translation="QLAAIGFFDGNLPCIFWARGYMAKTVMETWTFRNGKLNRLWKFD
                     TSEDKKLKDYEGQGNHNIRIGDIDGDGKDEIIYGACAIDNDGKPLYSTKLGHGDALHL
                     TDIDPSRPGLEVWDCHEFAPNEAGSSLSDARTGELLVKIPSTSDVGRCMCADIDPRHE
                     GCEVWSSQSGGLLSCKGEKVSNNTPSINMAVWWDGDLNRELLDGTRITKWTGNNTSVL
                     FDGKDKGILSNNGTKANPCLVADIFGDWREEVIWRSADNNEIRIYVTPYSTAYRFPTF
                     LQDIIYRHSVETQNVGYNQPTQPGFYFGSDSDETMEKLFSRLEKLDWKEEMSDDCTND
                     WKDKWFLDGEKAKITNSISGMTFCAGETPASDADHAVLWTKKSFGGNVKIEYDFTRLD
                     SVNRFVNILYIQAQGEGKAPYIKDISMWNDLRKIPAMSVYFNHLNTLHISYAAYENDE
                     NYNKNSEYIRARRYLPENGKGLTDTDLQPEYLSPMLFQPNKKYHLTVIKYNDILLMKV
                     NCEDKNRYFFFSTENFPPLKDGRIGLRQMSTRKSLYGNFKVYTTDNE"
     assembly_gap    1890..1989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1990..2634)
                     /locus_tag="PKH58_05075"
     CDS             complement(<1990..2634)
                     /locus_tag="PKH58_05075"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88431.1"
                     /translation="MKKKVFILFINLLFAGYIWGDVKIPDYNIRQIEKMNRGLVAIHS
                     GNGEVSVTWRLFKEDASAISFDLYRKTAEKRVKLNKSPLWFSTYFADKNVNTSEDNEY
                     QLFVHDKKNNDVLVATYLLTAEKAKIPYISIPLVPLKFKDAELFQPNDASVGDLDGDG
                     ELEIVLKRVGAFYACSQSGLCPEGNILEAYKLNGKRLWQINVGPNIRQGAQYFQF"
     gene            complement(2746..6339)
                     /locus_tag="PKH58_05080"
     CDS             complement(2746..6339)
                     /locus_tag="PKH58_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HNX88432.1"
                     /translation="MKSKFLKISVIFLAVFLCTFLTCQIYAQLVSFPEAEGFGKYALG
                     ARASSAPQVVHVTNLNDSGTGSFRDAVSASNRIVVFDVAGVIKISSRIVVSSNIYIAG
                     QTAPGEGITVYGNGFSFSNADNVICRYLRIRMGAVGDSGKDALGIAEGSNMIFDHVSV
                     SWGRDETFSVSGEACVNITIQNSIISQGLLSHSAGGLIQTDGGVTLYRNLYVDNDTRN
                     NKIKGVNQYVNNIVYNWSSGAYIMGGDSEGQSYANCESNYFINGPLNSVAALGGANAN
                     FHIYADDNWWDKNRNGIVDGYLVPQNEYSGGPDFQTVRYNYPVLPKWSANMLIDSVLP
                     FVGASLPYRDLADCYVVNEVRSFGKGGNVISNENELSIGIPTSWNVWTGTKRTDTDND
                     GMPDAWEDSNGLNKVDATDAVKKAVNGYLNIENYINGITSSYSQDFLRAPLNLTTDSI
                     TQNNIYLTWFDFTDKEIGYCIEQKIGGTFTEITRTNKNISTFNVAGLTPEQSYVFRVR
                     GYNAGGYSTYSNELTVKTKPVPVAVTDLTTYTPDLIWKGQNDNSWNKTSANWSGGSEI
                     FTDNSKVLFPELVNTATISITEPVSIGTLVVNASNDYTLSGSSISGVGSVNKTGTGKL
                     SLMSDNNYTGATVLYRGTLELNKIANGGLPSSIGASPNYDFNLVLKGGKLSYTGASVS
                     TDRSIVIENNPEISIETNGTVLTQNGIITGTGSLTKSGAGTLLSKNINTYDGTTTITN
                     GTIEYNGVDNINASLGAGKSFVLNGGRLKTSGGSTTNYENYYADIQVLDGNVSAFEPF
                     RNCYIYSKFSGSGKLYFDINYVREYVQGDWTQFSGIVYARGLGTATDGSQFMLNNTLG
                     IPNGRVITSGNTKIVCWKNAGTMYLGGLSGAAGTYLAGADKQNNAATMTWIVGGTGTD
                     ETFAGIINNECSNGSYKGTTSIVKEGNGYWRLTGANIYSGTTTVRGGMLIVNGAHTGA
                     GKITVQEGGLLGGKGTLPGAVVVLSGGVIQAGDPMAIPSNIATMTVGPLTLNAGSQTN
                     MEINPTTMTSDKIASSGAVVFGGKLNIKNTTTQLLKDGDQFTLFSGTSFSGNFSEIIP
                     SEPGDGLKWVFSNGVLKVVSDANGIVNPYQELIKISPNPANTFTAIRLPEKFREITLK
                     VMDFSGKKIKHQVFTETNEISLDCRNLQKGTYLICLYSNNTIIYSDKLIKK"
     gene            complement(6346..9402)
                     /locus_tag="PKH58_05085"
     CDS             complement(6346..9402)
                     /locus_tag="PKH58_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HNX88433.1"
                     /translation="MNRKKIVTTFLVGLLAFSCLIAQDIKTGQRTKYNFNSGWLLQLG
                     DVKGAEKPSNIDKSWKKITLPYAFNQDEAFKLSISEHTDTVVWYRKHFRLPKTASGQK
                     IFLEFEGIRFGGEFFLNGKSIGWHENGVMAFGFDISEIVNYSGENVLAARIDNSWRYK
                     EHTTGSGYQWNDRNFYANYGGIPKNVYLHVTPKLYQTLPLYSNLQTTGTYVYAREIDI
                     TNKSAQIFAETQVKNELPAEKNVELEVKIEDPDGKIIKTFKSKPEKIASGEMKSIKAH
                     NKVSDLHFWSWGYGYLYNVYTTLIVDGKPSDVVKTRTGFRKTRFDKGMFWLNDRVLQI
                     KGYAERTTNEWPAQGQSVPAWLSDYSNKMMVESNANTVRWMHVTPWKQDVESCDRVGL
                     IQLMPAGDSEKDVEGRRWEQRVELMRDAIIYNRNNPSVIFYEGGNETISETHITELKA
                     LRDKYDPYGGRVMGSREMLDSKIAEWGGEMLYINKSAQKPLFATEYCRDEGLRKYWDN
                     YSFPYHKDGEGSKYYRSVANAEPQKNIDTRPYNRNQDSFFEELITRWWDYYRVRPGTG
                     TRVSSGGLKIHFHESNTHWRGAENYRRSGVVDAMRVPKDAFFAHQVMWNGWVDIEKNG
                     SYICGHWQQAYNPPVDGKNPTPRETKDVMVVSTGEKVELFVNGKSQGFGERSAGFLFK
                     FKDIKWEEGTVEAVSYDNERSIISKIKKETAGKPEKITLKLINAPDGFKADGVDLALI
                     EIEVVDKDGRRCPLDNSLIDFDLQGNAEWRGGIAQGKEGNYVLEKQLPVECGINRVLI
                     RSTTKAGKIKLTASTPDNSLKPASIEFSSVKINEKEGLSTYISSNYQPVNLSRGETPK
                     GVSFTIKRHSVEIVGATAGSNEADVAKSFDDNELSEWRNDGRISSGWIKYELEHDATV
                     NEIELKLTGWRMRSYPIQIFVDGKKVWEGETEKSLGYINIPFKPTKGRFVTIRITGAT
                     EEKDAFGGIVEVAAKSAGELDLYKDPDGANSKGELRIVEAEMYELK"
     gene            complement(9420..12515)
                     /locus_tag="PKH58_05090"
     CDS             complement(9420..12515)
                     /locus_tag="PKH58_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNX88434.1"
                     /translation="MKTRQSHRNQSVVKAMLFCFYIFSCISVSAHADWDGAKWIGAAE
                     EDLPLYSHYLSVLRINYTVQFSDHSASAKAEFIYGANDERLMDRNRNIYNIQSKRDSS
                     FIRIELDATVLPAKLNIYRKGYAPTDNGNVPLFTFEISKSIINQENKFAPHVVSIHSN
                     MGITQIFIDDDKKEEVFANFVLNPYGRGGDFFAYPVLGDIGFSEKISDVEIRNFREPS
                     NVLRSFRKSAGNIIFNPSANSMPMLRSNFEITGPKIDKATLCVTARGIYDFYINNKRI
                     NDGYFNPGASQYNKTHFYQVYDVTKFLRKGKNTMGAILGEGWWSGAITYAGESWNFFG
                     DRQSLLAKLVITYADGTETVIPTSPDTWQYYNNGPTITAGFFNGEVYDARREVAGWSN
                     SDFEPENWKKAKEITLEGNTAAFDYSAYSLVELTGQTVKPIMKLTAVSVTEVRPGVFV
                     YDMGQNFAGVPEIHLSGMKQGTKINLRFAEVKYPDLPEYKQNTGMIMLENIRSAMAQD
                     IYICKGGDEEVFSPRFTYHGYRFVEITGLEKALALKDIKGIVLSSIDNVTADYECSNP
                     EVNRLWQNIKWSAFSNFVSIPTDCPQRNERLGWSGDISVFSRTAVYMADVRKFLSRHL
                     RAMRDCQNPDGRFPDVAPFDFGFGGLLWGSAGITVAWEIYLQYADTELLAEHYDAMKR
                     YIEFVQRNYIDTQTNVIVQNRTGLDLGDWLSPEEEKNDRSLLWEAYFIYDLDIMQRIA
                     LKLNKKDDAAMFAQISSERKSFFNETYIDKTTGKTISSGYYSSRIYVPEKGKVVDTQS
                     SYALPLAFGIVNKENKPLFLKNFIETIRRENILDKKQTCPPYSLMTGFIGTAWISKAL
                     SDNGFTADTYRLLQQTSYPSWLYPVEQGATTIWERLNSYTHTDGFGGNNRMNSFNHYS
                     FGAVGAWLIENSLGIRRDENNPGFRHFILSPEIDITNKMAYARGYYNSPYGKIESMWK
                     TSEGKTIYNFTVPKGTTATLYFPLSAKYTLKKGKTANESQENGKMKFELKEGKYEFEA
                     K"
     gene            complement(12524..13759)
                     /locus_tag="PKH58_05095"
     CDS             complement(12524..13759)
                     /locus_tag="PKH58_05095"
                     /inference="COORDINATES: protein motif:HMM:NF025934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HNX88435.1"
                     /translation="MKNTIIIFSILFSINFIGKAQPVMETGGQPMPDEWIDKDTGHKV
                     IKLTRREGTNMSFYFHNNPFVGNKMIFVGGDKYKGKDITGREIYNFDGRNLQMYWVDL
                     NTLKTEQLTSENFPVRTEMVCSKTHEIFYQHKDTVFAFNLDTRQSRQIYVNHTKKEKF
                     SVTTVNADGTLLAGAFSSPKEAEILKKYPEKSQFFGRIFEAKLPRTLFTINTKTGKLT
                     RIYKEKAWLNHIQFSPTDAHLLMFCHEGHWHKVDRIWTIDVVKKDKPQLRHKRSLPME
                     IAGHEWFGSKGDAIYFDLQKPRGKTFFVAKTYLATNIEQQYELQRNEWSVHYTTSWDE
                     NLLAGDGGDSTSVAKAEDGKWIYLFKPVVNTLKSTRLVNMKNHNYRLEPNVHFSPDNK
                     WIIFRANFEGEENVYAVEI"
     gene            complement(13771..15150)
                     /locus_tag="PKH58_05100"
     CDS             complement(13771..15150)
                     /locus_tag="PKH58_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNX88436.1"
                     /translation="MKSFHAFFPAFVGLTTFTLPANGQKQKDVRPNIIFILADDMGYG
                     DLACYGNQIIKTPNIDKLAKEGVRFTNAYAGSAVSSPSRCSLLTGKHTGHTTIRDNFA
                     KKGGITGLKNGNKIQRANILAEDTTIATILSAAGYRTCLVNKWHLDGFNPNAGPLDRG
                     FDEFYGWLVCKDESNLPYYYPALRYYNRELKEIPENKNNARGIHDSDLSVRESIDFIE
                     RNKKNPFFLYLAFDVPHEPYVINDTEPYKSMNLSDPAKLYAALITHTDQAIGRLIDYL
                     DKNNLRENTLIIFTSDNGAATMAPIPELNCNAGIKGHKAQLYEGGIKEPFIVNFPKRI
                     KGGKVLKNLIYFPDVMPTLAGYANAKLPRNTDGISIKPLLEGKKQITDDRTLYWEFPG
                     TQVAVRKGKWKAVSVKKGVELELYDISKDPNETNNLADKYPGIVSELANEIKRQHTPS
                     PYWPIDGEE"
     gene            complement(15216..16448)
                     /locus_tag="PKH58_05105"
     CDS             complement(15216..16448)
                     /locus_tag="PKH58_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNX88437.1"
                     /translation="MKRNLLLFSILVFSAGSLFSQNRTDEEAIRAVADNILKQPVTQF
                     VGVDDGTVYNSTAEIPAGKDVKFRSPLTEWHYSNGVLDIAMISLGKYLNEPKYINYAK
                     NHVAFGFNNYEYFKNTFRNDRKHWSWPFGQLWNMKELDDCGAMGAAVVGVYQLENKKE
                     YFDYIQKAANHIMNGQIRLKDGTLCRTFPRKMTVWADDAYMATSFLTEMGKLSGENKY
                     FDEAAKQLINIDKYLWCPEKQLYYHCYYTDLKRNGVAFWGRANGWITVSLANLIEAMP
                     ANHPKRAKLISILEKQIVGFAYYQNANGMWNQLLDKSDSYDESSVTAMFVYGVAKAVN
                     NKWIDAGYASIARAGWNILKNQEITPDGRFTNVCVGTGIGYDAPFYFNRPVGDNEKHG
                     LGLIIDTAVEIMKLNKKK"
     gene            complement(16568..17845)
                     /locus_tag="PKH58_05110"
     CDS             complement(16568..17845)
                     /locus_tag="PKH58_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HNX88438.1"
                     /translation="MKQYLSVFFVFVVASAWSMQMLAVDYKFSFNKQDTKKGFVAISE
                     KTFYGANSAYGYDLNTVQNGKDPFFFSVDLPEGNYRVKLTLGNQEFETNTTVRSESRR
                     LMLENIKIPKGKFVTREISVNLRNTKIGTRDSVKIKPREMGKLNWDNKLTLEINGIHP
                     SLTRLEISSADNIPTVFLAGNSTVVDQDDEPWCGWGQILPRFMSPKIAIANYAESGEA
                     GNTFIRSNRFAKLLHEMKKGDYLFIEFGHNDMKQKGENAGAFKSYKQSLVYMIDETRK
                     KGGTPVLVTPMNRRRFDDNGKVINTLEDFPDAVRLTGKELNVMVVDLNAMSKVLYEAW
                     GPNESVKAFVHYPAGTFPGQKTDLADNTHFNTFGGYEICKCILKGLKDNKSPLSKYIV
                     KDFTTFDPAKPDKFSDFSIPATPFSSTEKPDGN"
     gene            complement(17864..18178)
                     /gene="rhaM"
                     /locus_tag="PKH58_05115"
     CDS             complement(17864..18178)
                     /gene="rhaM"
                     /locus_tag="PKH58_05115"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HNX88439.1"
                     /translation="MKREAFKMFLKPGFEAEYEKRHSEIWPELEKLLKDAGVYDYSIF
                     WDKDTNVLFAVQKTSGDGNSQDLGGNPIVQKWWAYMADIMDTNPDNSPVSIPLKEVFR
                     MD"
     gene            complement(18197..>18702)
                     /locus_tag="PKH58_05120"
     CDS             complement(18197..>18702)
                     /locus_tag="PKH58_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNX88440.1"
                     /translation="GIKKGDIVFIQFGHNDGGPLNTGRARASLKGTGENSETVIMEST
                     GGPEEVFTFGHYLRIYIRQAKAMGAVPVVLSHTPGNSWEGGKMLRNSDTYGGWSKEVA
                     EQEGVYFIDMNDIVARKCESLGQEKTSELYKDRVHTSYEGAMLNCKSLIEGIRNVPEL
                     TLNKYIK"
BASE COUNT         5035 a         4104 c         3370 g         6093 t
ORIGIN      
        1 ctactgtctg ccctttcata atcgtagcgt atggttctgt tattgaaccg gttcctgtag
       61 catcgtttcc attggtagca atataatagg ttctagcatt tacagtggat aatcctgata
      121 acaaaacaat aaaaagtatg attatttttt tcattatagt aaattataat tgaatttttc
      181 caatcatgtt tttgaaaaga tgtcctttac taaaaaatct cattcattat cagtagtata
      241 aactttaaaa tttccgtaca acgattttct tgttgacatt tgtcttaaac caattcttcc
      301 atcttttaga ggagggaaat tttcggtcga aaaaaagaaa tatcgatttt tgtcctcaca
      361 atttaccttc atcaaaagaa tatcattata ttttataact gtcaaatggt attttttatt
      421 tggttgaaat agcattggcg agagatattc cggctgtaaa tcggtatcgg ttaaaccttt
      481 gccgttttcc ggtaaatatc ttcttgctct gatgtattct gagtttttat tataattctc
      541 gtcattttca tatgcggcat aactaatgtg taaagtattc aaatgattaa aataaacact
      601 cattgcagga atttttctta aatcattcca cattgaaata tctttgatat aaggtgcttt
      661 tccttcgcct tgcgcctgaa tgtaaagaat atttacaaaa cgatttacgc tatctaatcg
      721 agtaaaatca tattcgattt ttacatttcc accgaaactt tttttagtcc aaagcacagc
      781 gtgatcggcg tctgatgctg gtgtttcacc agcacaaaaa gtcattcctg aaattgaatt
      841 tgtaattttc gccttttcac catctaaaaa ccatttatct ttccaatcgt tggtgcaatc
      901 atcgctcatt tcttctttcc agtctaattt ttctagtctc gagaagagtt tttccatcgt
      961 ttcatcacta tcggaaccaa aataaaaacc gggttgggta ggctgattat aaccaacatt
     1021 ttgtgtttca acactatgcc tgtaaattat atcttgcaaa aaagtcggga aacgatacgc
     1081 tgttgaataa ggtgttacat aaattctaat ttcattatta tccgcagaac gccagataac
     1141 ctcctcacgc caatctccaa aaatatctgc gaccaaacac ggatttgctt ttgttccgtt
     1201 atttgaaagt attcctttgt cttttccatc gaacaaaaca gaggtgttat tacctgtcca
     1261 ttttgtgatt cttgttccat caagcaattc tctatttaaa tctccatccc accaaacagc
     1321 catatttatc gatggagtat tattcgaaac tttttctcct ttacacgaaa gaagaccgcc
     1381 ggattgcgac gaccaaactt cacacccttc gtgtcgcggg tcaatgtcgg cacacataca
     1441 tcttcctaca tctgaagttg acggaatttt aaccaacaat tctcctgttc tggcatcact
     1501 aagactggaa cccgcttcat tcggagcaaa ttcgtggcaa tcccatactt ccaatcccgg
     1561 acgagaagga tcaatatctg tcaggtgcaa agcatcaccg tgacctaatt tagttgagta
     1621 tagcggtttt ccatcgttgt ctattgcgca agctccataa attatttcat cttttccatc
     1681 accatcaata tctccaattc ggatattgtg atttccttgc ccttcgtaat cttttaattt
     1741 tttatcttca gatgtgtcaa atttccacaa tctgttgagt ttaccatttc tgaatgtcca
     1801 ggtttccatc actgtttttg ccatataacc tcgtgcccaa aatatacacg gcaaatttcc
     1861 atcgaaaaaa cctatcgcag ctaattgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnna aactgaaaat attgtgcacc ctgtctgata tttggtccga catttatttg
     2041 ccataaccgt ttcccgttta atttataggc ttccagaata ttcccttcgg ggcataggcc
     2101 tgattgcgaa caggcataaa atgctcccac tctttttagt acaatttcca gttcaccatc
     2161 accatccaaa tcgccaactg aagcgtcatt gggttgaaat aattctgcat ctttaaattt
     2221 taacggaaca agcggaatag aaatatacgg aatttttgct ttttcagcgg ttaacaaata
     2281 agtggcaaca agaacatcat tgtttttttt atcatgaaca aataattgat attcattgtc
     2341 ttcggaggtg tttacatttt tatcagcaaa atatgtagaa aaccacaaag gtgatttatt
     2401 cagtttgaca cgtttttctg cggtttttcg gtataaatca aaacttatag cggaagcatc
     2461 ttctttaaaa agacgccagg taacagaaac ctcaccattt cctgaatgaa ttgcaacgag
     2521 tcctctgttc atcttttcga tttgcctgat attataatct ggaattttta catcgcccca
     2581 aatatatcct gcaaacaaaa gatttatgaa taatatgaaa acttttttct tcatttctta
     2641 aacgtgacac aaaacctctt tttcaatttt tatctgattg attatcaatc cttcatcatt
     2701 aacaaaaatc cctccgaatc ttcccgatag gtcatcagta taaatttatt tttttatcaa
     2761 tttatcggag tatattattg tgttgttgct gtataaacaa atcagataag ttcctttttg
     2821 aagatttcta caatctagac tgatttcgtt tgtttctgta aatacctgat gtttaatttt
     2881 tttcccactg aagtccatca ctttcagtgt aatttccctg aatttctcgg gcaatctgat
     2941 ggcagtaaag gtatttgcag gatttggcga tatttttatt aattcctgat acggattcac
     3001 aataccgttt gcatctgaaa ccacctttaa aactccgttg ctgaaaaccc atttcaagcc
     3061 atccccgggt tctgaaggga taatttcact aaagttacct gaaaaagatg tacctgaaaa
     3121 gagtgtaaac tggtcaccat cttttaacaa ctgagttgtg gtatttttta tgttcagttt
     3181 tccaccaaaa accacagccc cgcttgaagc aattttatcc gaggtcattg tcgttggatt
     3241 gatttccata ttagtctgac ttccggcatt cagtgtaagc ggacctactg tcatggttgc
     3301 aatattggac ggaatagcca tcggatcacc ggcctgaata actccgccag acaaaaccac
     3361 gacagcaccc ggcaacgttc cctttcctcc gagaagccca ccctcttgaa cagtaatttt
     3421 tcctgctcct gtgtgtgcac catttacaat cagcattcct cctctgacgg ttgttgttcc
     3481 actgtagata tttgctcctg ttaatctcca gtagccgttt ccttctttca caatgcttgt
     3541 tgttccttta taacttccgt tggaacactc gttattgata attccggcaa aggtttcatc
     3601 ggttccggtg ccacctacaa tccaggtcat agtggcagca ttgttttgtt tatcggctcc
     3661 tgccagatat gttccggcag cgccagaaag tcctcccagg tacattgtac ctgcgttttt
     3721 ccaacagaca atttttgtat tacccgaagt aataactctt ccgtttggaa ttccaagtgt
     3781 attattcaac ataaactgac ttccatctgt ggccgttcct aaaccacgtg cgtaaacaat
     3841 tccgctaaat tgcgtccaat cgccctgaac gtattctcta acgtaattta tatcaaaata
     3901 aagttttcca cttccactga atttgctgta aatatagcag tttctaaatg gctcgaaagc
     3961 tgaaacattt ccatcgagaa cttgaatatc tgcatagtaa ttttcgtaat ttgtagtgga
     4021 accgccactt gtttttaatc ttcctccatt taaaacaaat gattttccag cacccaaact
     4081 tgcattgata ttatcgactc cgttgtactc aatagttccg ttggtaatgg ttgtagttcc
     4141 atcgtaagta ttaatatttt tactcaataa ggttcctgct cccgattttg ttaaacttcc
     4201 ggtgccggta ataattccgt tttgagtgag gacagtgcca tttgtctcaa tcgaaatttc
     4261 aggattattt tcaattacaa tagaacgatc ggtagaaact gaagcgcctg tgtaactcag
     4321 ttttcctcct ttcaacacca gattgaaatc gtaattaggt gatgctccaa tggaacttgg
     4381 caggccaccg tttgctattt tatttaattc caatgttccc cggtacagaa ccgtagctcc
     4441 ggtatagtta ttatcagaca tcagcgacag ttttccggtt ccggttttat tgactgaacc
     4501 tactccggag attgatgatc ccgacaaggt ataatcgttt gaggcattta cgactaatgt
     4561 acctattgaa accggttcgg tgatggaaat tgttgcggta tttactaatt cgggaaataa
     4621 aactttcgaa ttatcggtaa aaatttcaga tccgccactc cagttagcag aagttttgtt
     4681 ccagctatta tcattctgac ccttccatat tagatctgga gtatatgttg tcaaatctgt
     4741 tacagcaaca ggaacaggtt ttgttttcac cgtcaattca ttggaataag tggaataacc
     4801 gcctgcatta taacctcgaa ctctaaaaac ataagattgt tccggagtta aacctgcaac
     4861 attaaaagta ctgatatttt tgttggtacg tgttatttct gtaaaagttc caccaatttt
     4921 ttgctcgata caatacccta tttctttatc ggtaaaatca aaccaggtta aataaatatt
     4981 attttgagtg attgagtcgg tggtcaaatt gagcggagca cgtaaaaaat cctgagaata
     5041 cgatgatgta ataccattga tataattttc gatattcaaa tatccgttta ccgctttttt
     5101 taccgcatca gtagcatcaa ctttatttaa tccatttgaa tcttcccaag catcaggcat
     5161 tccatcgtta tccgtatcag tgcgttttgt tcccgtccaa acattccaag atgttggtat
     5221 tccaatagat aattcatttt catttgaaat tacattccct cctttcccaa aagaacgtac
     5281 ttcattcacg acataacaat cggctaagtc gcgataaggc aatgaagctc ccacaaaagg
     5341 aagtacagaa tcgatcaaca tattggcact ccattttggg agaaccggat agttataccg
     5401 aacagtctga aaatcaggac cacccgaata ctcattttgc ggaacaagat atccgtcaac
     5461 tattccattt ctgtttttat cccaccagtt gtcatcagca taaatatgaa aattggcatt
     5521 tgcaccacct aatgctgcta ctgaatttag cggtccattg atgaaataat tgctttcgca
     5581 gttggcatac gactgtcctt cggaatcgcc tcccataata tatgctcctg acgaccagtt
     5641 atatacaata ttattgacat attgattaac acctttgatt ttattattcc gggtatcatt
     5701 atcaacatac aagttgcggt aaagtgtaac acctccatcg gtttgaatta atccgcctgc
     5761 ggagtgactt aataatcctt gcgaaataat cgaattttga atggtaatat tgacgcacgc
     5821 ctctcccgaa acagaaaaag tttcatctct accccacgaa acagaaacgt gatcgaaaat
     5881 catattgctt ccctcggcta ttcccaaagc atcttttccc gaatctccaa cggcacccat
     5941 tcttatccgc aaatacctgc aaataacatt atcggcattt gagaacgaga atccgttgcc
     6001 ataaacagtt attccttcac caggtgctgt ttgtccggca atgtagatat tggatgaaac
     6061 aacgattctg ctggatattt taatcacacc ggctacatca aataccacaa ttcgattcga
     6121 agcactgacc gcatcgcgaa atgagcctgt tccggaatca ttcagattag taacatgaac
     6181 tacttgtggc gcagatgatg ctcttgctcc caatgcatat tttccaaaac cttcagcttc
     6241 cggaaatgat acaagttgtg cataaatctg acaagtcaaa aacgtgcata aaaaaacagc
     6301 caggaaaata acagatattt ttaagaactt tgatttcata aaattctatt ttaattcata
     6361 catttcggct tctactattc ttaattctcc ttttgaattt gcgccgtcag ggtctttgta
     6421 taaatcaagt tctcctgccg attttgccgc cacttccaca atgcctccaa aagcatcttt
     6481 ttcttcagtt gcacctgtaa ttcttatagt tacaaaacga ccttttgtcg gcttgaaagg
     6541 gatattaatg tatccgagcg atttttcggt ttcgccttcc cacacttttt ttccgtccac
     6601 aaaaatctga atgggataag aacgcattct ccaaccggtc agtttgagtt ctatttcatt
     6661 tactgtggca tcatgctcca gctcgtattt tatccagcca gaggaaattc ttccatcatt
     6721 cctccactcc gacaattcat tatcgtcaaa gcttttggct acgtcggctt cgttgcttcc
     6781 ggcagttgct ccgacaattt ccacagaatg tctttttata gtaaaagaca ctcctttggg
     6841 agtttcgcct cgggaaagat ttactggttg ataattggac gaaatatagg ttgataaacc
     6901 ttctttttca ttaattttta ctgatgagaa ttcaattgaa gccggtttca gggaattatc
     6961 gggggtgctc gctgtcagtt taatttttcc tgctttggtt gtggaacgaa tcagcacacg
     7021 gttaatgcca cattctaccg gcagttgttt ttcgagtaca taatttcctt cctttccctg
     7081 tgcaattcct ccccgccatt cagcatttcc ctgtaaatcg aaatcaataa gcgagttatc
     7141 gagcggacaa cgtctgccat ctttgtccac cacttcaatt tcgatcaatg ccagatcaac
     7201 tccatcagct ttgaacccgt cgggtgcatt tatcagtttc agcgtgattt tttcgggttt
     7261 tcctgcagtt tcttttttta ttttgctgat aatactcctt tcattatcgt agcttacagc
     7321 ttccacagtt ccttcttccc attttatatc tttgaatttg aaaagaaatc ctgcgcttct
     7381 ttcaccaaat ccctgcgatt ttccattgac gaaaagttcc actttttcac ctgtagaaac
     7441 caccataaca tccttcgttt ctctcggagt tggatttttt ccatcaacag gaggattgta
     7501 tgcttgttgc cagtgtccgc aaatataact tccatttttt tcaatatcaa cccagccgtt
     7561 ccacatcacc tgatgtgcaa aaaaagcatc ttttggaaca cgcatggcat ccacaactcc
     7621 gctcctgcgg tagttctcag ctccgcgcca gtgtgtattt gactcgtgga aatgaatttt
     7681 cagcccaccg ctgcttactc ttgtgcctgt gcctggacgt acccggtaat agtcccacca
     7741 gcgggtgatt aattcctcga aaaagctgtc ctgattgcgg ttgtagggac gagtgtcaat
     7801 atttttttga ggttcggcat tcgcaaccga acggtaatat ttcgatcctt caccgtcttt
     7861 gtggtaagga aacgagtaat tatcccagta ttttcgcaat ccttcatccc ggcagtattc
     7921 ggttgcaaaa agcggttttt gagcactttt attgatgtaa agcatttcgc ctccccattc
     7981 agcaatttta ctgtcgagca tttcacgtga acccattaca cgtcctccat atggatcgta
     8041 cttgtcacgc agggctttga gttccgtgat gtgtgtttcg ctgattgtct cgtttccacc
     8101 ctcataaaaa atcacgctcg gattattccg attgtaaata atggcatcgc gcatcagttc
     8161 cacacgttgt tcccagcgac gcccttccac atctttttcc gaatctcctg cgggcatcaa
     8221 ctggataagt cccacccgat cgcaactttc cacatcctgc ttccagggtg taacatgcat
     8281 ccagcgcacc gtattcgcgt tgctttccac catcattttg ttactgtaat cgctcagcca
     8341 cgccgggacc gactgaccct gagcaggcca ttcgttggta gtgcgttcag catagccttt
     8401 tatctgcaaa acacggtcgt ttagccagaa cattcctttg tcaaaacggg tttttcgaaa
     8461 tccggtacgg gtttttacca cgtccgaggg ttttccgtcc acaatgagtg tggtgtaaac
     8521 attgtacaga tatccataac cccacgacca gaagtgcaag tctgaaactt tgttgtgtgc
     8581 tttgatactc ttcatttctc ccgaagctat tttttcgggt tttgatttga atgttttgat
     8641 aatttttccg tccggatctt caatttttac ttccagctcc acattttttt ctgccggtaa
     8701 ttcgtttttc acctgagttt cggcaaaaat ctgtgcagat ttatttgtaa tgtcgatttc
     8761 gcgggcatac acgtaggttc cggttgtttg cagattgctg taaaggggta aagtctgata
     8821 cagttttggt gtaacgtgta aatatacatt tttagggatt cctccgtagt ttgcataaaa
     8881 attccggtcg ttccactgat agccggaacc tgttgtatgt tccttatatc gccaggagtt
     8941 gtcaatacgt gccgctaaaa cattttcgcc ggaataattc acaatctccg aaatatcaaa
     9001 tccgaaagcc attaccccgt tttcgtgcca gccgattgat tttccgttca ggaaaaattc
     9061 accgccaaaa cgaatacctt caaattccag aaatattttt tgaccgctgg cagtttttgg
     9121 caatctgaaa tgcttgcgat accagaccac cgtatcggta tgttccgaaa tactcagttt
     9181 gaatgcttca tcttgattaa aagcgtacgg aagtgttatt tttttccagg atttatcaat
     9241 attactcggt ttctcagccc cttttacatc accaagctgt aaaagccaac cggaattgaa
     9301 attgtatttt gtacgttgac cggttttgat gtcttgcgca atcaggcagg agaacgccaa
     9361 aagaccaact aaaaaagtcg taactatttt ttttctgttc ataatatccc ggattttatt
     9421 tatttcgctt caaattcgta ttttccttct ttcagttcaa acttcatctt cccgttttcc
     9481 tgactttcat tggctgtttt tccctttttc agcgtatatt ttgcagaaag cggaaaataa
     9541 agcgttgctg tagttccttt gggtactgta aaattataaa tagttttgcc ttctgaagtt
     9601 ttccacatac tttcaatttt tccgtacgga gaattgtagt atccgcgtgc atacgccatt
     9661 ttattggtta tatcaatttc gggagagaga ataaaatgtc tgaaacccgg attattttca
     9721 tcgcgcctaa tgcctaaaga attctcaata agccaggcac caacagcgcc aaaagaatag
     9781 tgattgaaag aattcatccg gttatttcct ccaaaaccat cggtgtgagt ataggaattc
     9841 aggcgttccc agattgtggt tgcaccttgc tcaaccggat acagccacga aggatacgaa
     9901 gtctgttgta aaagccggta agtgtcggcc gtaaatccat tgtctgacaa tgctttagaa
     9961 atccaagcag taccgataaa acctgtcatc agcgaatatg gcggacaagt ttgctttttg
    10021 tccagaatat tctcacgacg aatggtttca atgaaatttt tcaggaataa cggtttattt
    10081 tctttgttta caattccgaa agccagtggc agtgcgtaag aagattgtgt atcaacaact
    10141 ttgccttttt caggaacata tatccgtgat gaataatagc ctgatgagat ggttttacct
    10201 gtggttttgt ctatgtatgt ttcattaaaa aaagattttc tttcagaaga aatttgtgca
    10261 aacatcgccg cgtcgtcttt tttattgagt tttaaggcaa ttctctgcat gatgtccaaa
    10321 tcataaatga aataggcttc ccatagcaat gaccgatcgt ttttctcctc ttcaggactt
    10381 aaccagtcgc ccaggtcaag tccggtacgg ttctgcacaa tgacgtttgt ttgcgtatca
    10441 atgtagtttc tttgcacaaa ctcaatgtag cgcttcatcg catcgtaatg ttccgcgaga
    10501 agctccgtgt cggcatattg caggtatatt tcccaggcaa cggtgatgcc cgcgcttccc
    10561 cagagcaatc cgccaaaacc aaaatcgaac ggagcaacat cgggaaaacg accgtcggga
    10621 ttctgacagt cgcgcattgc tctcaaatgt ctggaaagaa attttcgaac atcagccata
    10681 taaaccgctg tacgggagaa aaccgaaata tcaccgctcc agccaagtcg ttcgtttcgt
    10741 tgcggacagt cggtaggaat agacacaaag ttggaaaatg ccgaccattt tatattttgc
    10801 caaaggcggt taacttccgg gttggaacat tcataatcag cagtaacgtt atctattgaa
    10861 ctcagcacaa tgccttttat atctttcaaa gctaaagcct tttccagtcc cgttatttct
    10921 acgaagcgat aaccgtgata cgtaaaacgg ggcgaaaaaa cttcttcatc tcctccttta
    10981 caaatataaa tatcctgcgc catcgccgaa cgtatgttct cgagcatgat catccctgtg
    11041 ttttgcttgt attctggcaa atccggatat ttaacctcag caaaccgcag gttaattttt
    11101 gttccctgtt tcattccgga caaatgaatt tccggaactc cggcaaaatt ctgccccata
    11161 tcatacacaa aaacacccgg acgaacttca gtgaccgaaa ccgctgttaa tttcataatt
    11221 ggttttacgg tttgccctgt tagttctacc agcgaatacg ccgaataatc gaatgccgcg
    11281 gtatttcctt ctaatgttat ttcctttgct tttttccagt tttcgggttc aaaatcagaa
    11341 ttgctccagc ctgccacttc ccggcgtgca tcgtacactt cgccattgaa gaaaccagcg
    11401 gtaattgtcg gaccattgtt gtaatactgc caggtatcgg gcgaagttgg tatcactgtt
    11461 tctgttccat cggcataagt gatgactaac tttgccagga gcgactgacg atcgccaaaa
    11521 aaattccagc tttcgcccgc gtaggtaatt gccccgctcc accagccttc tccaagtatt
    11581 gcacccattg tattttttcc tttacggaga aattttgtaa catcgtaaac ctgataaaaa
    11641 tgtgttttat tgtactggga agcacccgga ttaaaataac catcatttat ccgcttatta
    11701 ttgatgtaaa aatcgtaaat gccgcgcgct gtaacacaga gggtggcttt gtcaattttc
    11761 ggaccggtaa tctcgaaatt actgcgaagc atcggcatcg aattagcaga cggattgaaa
    11821 atgatatttc cggcagattt tctaaatgaa cgcaacacat ttgaaggttc ccggaaatta
    11881 cgaatttcaa catccgatat tttttcagaa aatcctatat cacccagcac tggataagca
    11941 aaaaagtcgc ctcctcttcc gtacggatta agaacaaaat tggcaaatac ctcctctttt
    12001 ttatcatcat caataaaaat ctgtgtaatt cccatattgg aatgtattga aacaacgtga
    12061 ggagcaaatt tattttcctg attgatgatg gattttgaga tttcaaacgt aaacaaagga
    12121 acatttccgt tatcagttgg tgcatagcct ttccgataaa tatttaactt tgccggaagt
    12181 accgtagcat ccaattctat tcgaataaaa gaactgtccc ttttactttg aatgttgtaa
    12241 atattcctgt ttctgtccat caaacgctca tcgttagcgc cgtaaataaa ttccgccttt
    12301 gctgaagcag aatgatcaga aaattgtact gtataattaa ttctgagcac ggacaaataa
    12361 tgggaatata acggtaaatc ttcctctgca gcgcctatcc attttgctcc gtcccaatct
    12421 gcatgagccg atacactgat gcacgaaaaa atgtaaaaac aaaaaagcat tgctttaaca
    12481 acagactgat ttctatggct ttgtcgtgtt ttcatagttt tttttatatt tctaccgcat
    12541 acacattttc ttcgccttca aagttagctc taaaaataat ccatttatta tcgggcgaaa
    12601 aatgcacatt tggttccagc cggtaattat gatttttcat attcaccagg cgggtcgatt
    12661 ttaatgtatt tactacaggt ttgaaaagat aaatccattt cccgtcttcg gcttttgcta
    12721 cggatgtaga atcgccgcca tctccagcca acaggttttc gtcccaggat gtggtgtagt
    12781 gcacactcca ttcgttacgt tggagttcat attgctgttc aatattggtt gccaaataag
    12841 ttttcgcaac aaaaaatgtt ttaccgcgtg gtttttgcaa atcgaaataa atggcgtcac
    12901 ctttactgcc aaaccattcg tgtccggcaa tttccatcgg caacgaacgt ttgtgacgca
    12961 attgtggttt gtcctttttc accacatcaa tagtccaaat cctatctacc ttatgccagt
    13021 gtccttcgtg gcaaaacatc agtaaatgcg cgtcagttgg agaaaattgg atgtggttga
    13081 gccacgcttt ttctttataa atgcgggtta attttccggt ttttgtattg atggtaaaaa
    13141 gtgtgcgggg aagtttcgct tcgaaaatac gtccaaaaaa ctgacttttt tcgggatatt
    13201 tcttcagaat ttcagcttct ttcggggaag aaaaagcgcc tgccagcaat gttccatcgg
    13261 cattaacggt tgtaacagag aatttttctt tttttgtatg attcacatag atttgtctgc
    13321 tctggcgagt gtcaagattg aatgcaaaaa cggtatcttt atgctgataa aatatttcgt
    13381 gtgttttact acataccatt tcagtacgta ccggaaagtt ttcgctggtc agctgttctg
    13441 ttttaagggt atttaaatcc acccaataca tttgaagatt tctaccgtcg aaattataga
    13501 tttcacgtcc ggtaatgtct tttcctttgt atttatcgcc accaacgaat atcattttat
    13561 tgccgacaaa cggattgttg tgaaaataaa aactcatatt tgttccttca cggcgggtca
    13621 gttttattac tttatgtccg gtatctttgt caatccactc atcgggcatc ggttgtccgc
    13681 ctgtttccat tacaggttga gctttcccaa taaaatttat cgaaaaaaga atggaaaata
    13741 tgatgattgt gtttttcatt ttaatagaat ttattcttct ccatcaattg gccagtaagg
    13801 cgatggtgtg tgttgtcttt ttatctcatt tgcaagttcc gatacaattc cgggatattt
    13861 atctgccaga ttatttgttt cgttcgggtc ttttgaaata tcgtaaagtt ctaattctac
    13921 tccttttttc accgaaacag ctttccattt tccttttctt accgccacct gcgttccggg
    13981 gaattcccag tacaaagtcc ggtcgtcggt aatctgtttt ttcccttcca gcaacggttt
    14041 tatgcttatc ccatctgtat tacggggtag ttttgcattt gcgtaaccgg ctaaagtcgg
    14101 catcacatcc ggaaaataaa tcagattttt caacactttt ccacctttta ttctctttgg
    14161 aaaattgacg ataaacggtt ctttaattcc cccttcataa agttgagctt tgtgtccttt
    14221 aattcctgca ttgcaattca gttcagggat tggcgccatt gttgcagctc cgttatctga
    14281 agtaaaaatg atgagcgtat tttctcgcaa gttattttta tccagataat caatcaatcg
    14341 tccgatagcc tgatcagtat gagtgataag agcagcatag agcttggccg gatccgacaa
    14401 attcattgat ttatagggtt cggtatcgtt aatcacatac ggttcgtgcg gaacatcgaa
    14461 tgccagatac aggaaaaacg gatttttctt gtttctttca ataaaatcga tggattcgcg
    14521 tacagacaaa tcgctgtcgt gaattccccg ggcattattt ttattttcgg gaatttcttt
    14581 aagttctctg ttatagtatc tcagagccgg ataataatag ggaagatttg attcgtcctt
    14641 gcatacgagc cagccataaa actcatcaaa acctctgtcg aggggacctg cgttgggatt
    14701 aaacccatcc agatgccact tgttaacaag acatgttctg taacccgctg cactcagaat
    14761 agttgcaatg gtggtgtctt cggccaggat attggctcgc tggattttat taccgttttt
    14821 caaaccggtt ataccacctt ttttcgcaaa attatccctg atagtggtgt gtccggtgtg
    14881 tttcccggtc agtaacgagc aacgcgaagg ggaacttaca gccgaacctg cataagcatt
    14941 tgtaaagcgg acgccttctt ttgccagttt atcaatattc ggagttttga tgatttgatt
    15001 tccgtaacac gccaaatcgc cgtagcccat atcatcggca agaataaaaa tgatattggg
    15061 tcgtacatcc ttctgtttct gaccgtttgc cggaagagtg aaggttgtca gaccaacgaa
    15121 tgcaggaaaa aacgcatgga acgatttcat tgtattagtt ttataattgg tcagaaaatt
    15181 cactctgatt aaaaacctac cggtcaggca aataattatt tctttttgtt gagtttcatt
    15241 atttccacag ccgtatcgat aataagaccg agaccgtgct tttcgttatc tcccaccgga
    15301 cggttgaaat agaacggcgc atcgtaacca attccggttc ccacacacac attcgtaaag
    15361 cgaccatcgg gtgtaatttc ctgatttttg agaatattcc agccagctct cgctatactt
    15421 gcataaccgg catcaatcca cttgttattc acagcttttg ccacaccgta aacaaacatg
    15481 gcagtaaccg atgactcgtc gtacgaatca cttttatcaa gcaactgatt ccacatgccg
    15541 tttgcattct ggtagtatgc aaagcctacg atttgctttt ccaggatgga aattaattta
    15601 gctcgttttg gatgattggc gggcattgct tctatcagat tggccagcga aaccgtaatc
    15661 caaccgttgg cacgtcccca gaatgccacg ccgtttcgtt tcaaatcggt ataataacag
    15721 tgataataaa gttgtttttc gggacaccac agatatttat caatgtttat cagttgtttt
    15781 gcagcttcat cgaaatattt attttcaccc gaaagttttc ccatctcggt cagaaaagaa
    15841 gttgccatat aggcatcatc cgcccaaacg gtcatctttc tcggaaaagt tctgcacaaa
    15901 gtaccatctt tcagacggat ttgtccgttc atgatgtggt tagcggcttt ttggatgtaa
    15961 tcaaaatatt cctttttatt ttccagttga taaaccccta ctaccgcagc tcccatagca
    16021 ccgcaatcgt ccaattcctt catattccaa agctgtccga aaggccatga ccagtgtttc
    16081 cggtcattgc ggaaagtgtt cttgaaatac tcatagttat tgaatccgaa agcgacgtgg
    16141 tttttagcat aattgatata ttttggttca ttcaggtatt taccgagcga tatcatagct
    16201 atatccagca cgccgttgga ataatgccat tcggtgagcg gacttctgaa cttcacatct
    16261 tttccagcag gaatttcagc cgtactgttg tacacagttc catcatcaac tccaacgaac
    16321 tgcgttacag gctgtttgag tatattatca gccaccgccc tgatggcttc ttcgtcagtt
    16381 ctgttttgtg aaaaaagtga accagctgag aaaactaaaa tgctgaataa taataaattt
    16441 cttttcatat taattatata tctgcccact aaccccaaaa ggggggagct gagctggtta
    16501 ttaatcattt ccttgtatat ttagaattat ttgaatagtg ttctatcttt caatttataa
    16561 ctatttctta attgccatcc ggtttctctg ttgaactaaa aggagtagcc ggaatgctga
    16621 aatcactgaa tttatcaggt tttgccggat cgaaggtggt aaaatctttt acaatgtatt
    16681 tactcagtgg cgatttatta tcctttaaac ctttcaaaat gcatttacag atttcatatc
    16741 caccaaaggt attgaaatga gtattatcgg cgaggtcggt tttttgtccg ggaaatgttc
    16801 ctgccggata atgaacaaat gctttcaccg attcattggg tccccaggct tcgtacagca
    16861 ctttgctcat agcattgagg tccacaacca tcacattcag ttctttgccg gtcaaacgaa
    16921 ccgcatccgg aaaatcctcg agcgtattga tgacttttcc gttgtcatcg aagcgccgac
    16981 ggttcatggg cgtaaccagt accggtgtgc cgcctttttt acgggtttcg tcaatcatgt
    17041 acaccaaact ctgtttgtaa cttttgaatg ccccggcatt ttcacctttt tgcttcatat
    17101 cgttatgtcc gaattcaata aaaagataat cacctttttt catttcgtga agcagtttcg
    17161 caaaccggtt ggaccggata aaagtattgc cagcttctcc ggattcagca taattggcaa
    17221 ttgcaatttt cggactcata aatcgcggaa gtatttgtcc ccaaccacac cagggttcat
    17281 cgtcctgatc gacaactgta gaatttcctg ccagaaaaac agttggtata ttatcggctg
    17341 atgaaatttc caaacgcgtc agggatggat ggatgccatt gatttcgagc gtcaacttgt
    17401 tgtcccagtt taatttgccc atttcacggg gtttaatttt caccgaatca cgcgtgccaa
    17461 ttttggtatt ccggagattg acagaaattt cacgggtaac aaactttcct ttcggaattt
    17521 tgatgttttc cagcatcaat cttcttgatt cggatcgtac agttgtattt gtttcaaatt
    17581 cctggttacc gagcgtcagt tttaccctgt aatttccttc aggcagatca accgaaaaga
    17641 aaaaaggatc tttcccgttt tgaaccgtat tcagatcata accataagct gaattagctc
    17701 cgtaaaaggt tttttcgctt attgcaacaa agcctttttt tgtatcttgt ttattgaatg
    17761 aaaacttgta atctacagcc agcatctgca tactccatgc ggatgcaaca acaaatacaa
    17821 aaaagactga taagtattgt ttcatttaca atcaaaattt taatcaatcc atccggaata
    17881 cttccttcaa cgggatagaa acaggtgaat tatcgggatt ggtatccata atgtcggcca
    17941 tatatgccca ccatttctga acaatcgggt tgccgcccaa atcctgcgaa ttaccatcgc
    18001 cggaagtttt ttgcactgca aacaacacgt tggtgtcttt atcccaaaaa atggaataat
    18061 cataaacgcc cgcatctttc agcagttttt ccagttcggg ccagatttca ctgtggcgtt
    18121 tttcgtattc ggcttcgaag ccgggtttca aaaacatttt aaatgcttct cttttcatgg
    18181 ttgtattttt tggtttttat ttaatgtatt tattcagtgt cagttcaggt acatttcgta
    18241 ttccctcaat taatgattta caattaagca tggctccttc atacgaggta tgaacgcggt
    18301 ctttgtagag ttcactggtt ttttcctgtc cgagcgactc acatttgcgg gctacaatat
    18361 cgttcatgtc aataaagtaa actccttcct gttcagcaac ttctttcgac caaccgccgt
    18421 atgtatcact gtttcggagc attttccctc cttcccagga attgcccggc gtatgcgaca
    18481 gcacgacggg aacagcaccc attgctttcg cctggcggat ataaattctg aggtaatgtc
    18541 cgaaagtgaa cacttcttca ggcccgccgg tgctttccat gataactgtt tcggaattct
    18601 ctccggttcc tttcaacgat gcacgtgcac gtccggtatt gagtggcccg ccgtcattat
    18661 gtccaaactg gataaaaaca atatcacctt tttttattcc gg
//
LOCUS       DAOPYB010000043        18603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3116_length_18603_cov_9.359068, whole genome shotgun sequence.
ACCESSION   DAOPYB010000043 DAOPYB010000000
VERSION     DAOPYB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18603
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3116_length_18603_cov_9.359068"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2669
                     /locus_tag="PKH58_05125"
     CDS             <1..2669
                     /locus_tag="PKH58_05125"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX88441.1"
                     /translation="VAQTLRITGDYPQGNYKFTGSVTDVNGCVSSFFDVFLEMNTMPA
                     PPTIGNITQPTCALATGSIALTGLPSGNWTINPGNITGTGASYTVSGLAAGTYNYTVN
                     NGQCTSVASTDVVINTQPVTPAAPVVGTITQPTCVLATGSVVLNGLPSGNWTINPGGI
                     TGSGISTTLSGLATGTYNFTVNNGQCTSVATPDVVINTQPVTPAAPMVGTITQPTCIL
                     ATGSVVLNGLPSGNWTINPGNITGTGASYTVSGLAAGTYNYTVNNGQCTSVASTDVVI
                     NTQPVTPAAPVVGTITQPTCAVQSGSVVLDGLPSGNWTINPGNVSGSTVSTTISGLAP
                     GTYNFDVTNASGCTSAASANVDISANLITTAEAGPTVIYYGTPVQIGVVNNGSGTIIW
                     SPATGLNDPGIAQPLASPATTTTYTITVDNNGCVRTDTVTVVYGGIGHMIAGKTRYLK
                     MATAGNPAPNPPTYSPAIYNIDNVEVILKSSPGGAVIATTVSNANGEYQFTGVPDGDY
                     ILAYDHIPYPDTMQYVNHTNAVDLALLKYYVGHNPLTDPSRNFSEKHRKAANVDNNAS
                     INTVDVARISGKIGMPNDPAKNFPKGNWVAFDTLITMAGSDLNITLQTVAYGDYNASS
                     VKYQGAATNWGMVKELPDENIIVRSDESIMMSDPDYFEVPLRISTKMNELSAVGLELS
                     YPSDKYKLVSASMSNTGKKGDAIKINPTLEEIIAANNDLLVTDIDGVIRVVFATTDHF
                     DIAANEELVKLGFRSLIDPGRGELDFNLNGTGLIANQYGEINADAYLTMPKIFVQGDD
                     MEAGFELAGYPNPFSGDATLTYSIPENGTVKLSVYNAIGELVALLVNESQISGKHSAV
                     FSPVNLPAGMYTFKLEFTGSDKSKLMVLKMVH"
     gene            2841..6809
                     /locus_tag="PKH58_05130"
     CDS             2841..6809
                     /locus_tag="PKH58_05130"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNX88442.1"
                     /translation="MKKLFVFIVLFIALILSSNAQNYLTNTAAQPNHSIPKGPVVQTF
                     EGTFPAFDWSLTAGSGNWVKSSERNHTSGGSFSAMYNCWGINGTVPAYLQTPEMIVTA
                     GDATLTFWRNYYLLNNPNYGHAAELYIDISTDGGLTWTSGTTNYLDTITLNTWIQFTI
                     DLTANIGQNVILRFKAISDFGSYNIAIDDIAGPSQNLPPHDLEILALTPSFVLSGSTI
                     APEVEVRNMGDATETSFSLHISDGGAYNCTVPVTTSISYGTTCTVYCPDWTPADGNYT
                     LTANVILAPDTNTDNDTIKQNVLVSPDSWISWPAINSNRLTSYCASAGYTLSSGIYRF
                     LYSFGGTGGNSKMVTSYNLETGTCTFGTDMPAYSKMNSAVTVGDFMYVNRGRWGTTNY
                     NTFYKYDVLNNTWSTAANMPAALAYNAFAALNGNIYCVGGTTTGAVNTVYRYNIASNT
                     WTTASPISLVGGLYAGGLAIADNKLVYIQGVVNGALTGDVFIGTVDTTNYDVITWTTG
                     TPCPVGVFGIKAEPYTNGKIIYTGGSDNLSPLHVVPDSYIYDVNSDTWTRIQDKITPT
                     LGYAAATFNMGGKMRYFVSAGQSASGSLLDKVEFFNHQIIAAACTGTPQASDITGPVS
                     ICSGTEATLHLSSTYALSDITYQWAYSTNLGGPYNNLGTSSAQSTGNLTSSTYYICTI
                     TCTNSGLSYTTAEKMVGVYPLATLSIITDHLCEGDSSYILLSLEGTGPWLIHGLTEIA
                     ASGSTNIFPDIIASQSPYLVSFKPNENSYYHVESVTDNTTGCYTDTANWVMVTLAPVP
                     TANAGTDATYTSTPVLIGDPLNGPGIISWSPSEGLDNPTIAQPSASPLLTTTYNLTIN
                     NNGCIRTDAVTVASGGTGHAISGKTRYLKKANSGNPVPTPPSYNAVIYNIVNVEVKLK
                     TSPGGVLLSTTMSDANGVYQFTNVPDGNYILAYDHIPYDTMQFVNQVNAVDLALLKYL
                     IGHDTVIDPSRSFTAKHKKAANVDNNTTINTIDIARIASKVGLPYTPARNFPRGNWVT
                     LDTSVTVAGSNLNITLQTVSYGDYDASSSRYKDSASTWGMAKSLPDENIIILSDASIM
                     INDPEYFEVPLRISTKMNELSAVGLELSYPSDKYKLVSASMSNTGKKSDAIKINPTLE
                     EIIAANNDLLVTDVDGVIRVVYATTYHFEIAANVELISLGFRSLNNPGRGELDFNLSG
                     TGMIANQYGEINEDAYLTMPKIFVQGDDMEAGFEFAGNPNPFSGDATLTYNIPENGTV
                     KLSVYNAIGELVTLLVNESQSSGTHSAVFSPLNLPAGMYTFKLEFTGSNKSKCLVLKM
                     IH"
     gene            6984..9986
                     /locus_tag="PKH58_05135"
     CDS             6984..9986
                     /locus_tag="PKH58_05135"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX88443.1"
                     /translation="MNNRSNELLKICTLLQMGLSRICHWLKDSKGIFIMFIFLLLGIF
                     TFAQSYSYKHYTILDGLVQNQVTTLYQDYQGFIWVGTKAGASRFDGITFKNYTQQEGL
                     APGLVTGFFEDEEKNLFCITDNGYSILQNGNFKIVHIFNDKEIKYCHGPFCTKDKTCF
                     ILAFGYELYKCNKSKFELYYRFELKTEINEYYQNNEGREFVNTDSGFYEIYKNRQFSK
                     LFYQKPIGLKNCKNGSYIVVDGDNIKNKYPRGVYFLNNNLTTKIWKDTSKSLCNAFSV
                     LNDTNILIASNFDEWLILDKNGHKIDGDKNKDFVLITSFLEDKEGNIWIGDESGLFRL
                     QSFAFRNYNEASGLEKYIWTILEAPDSTIFFASFGGKLMKLKDDKIERVIGYERFLNS
                     GTQFYMGGTRTANGHLLFPVNNGTILDYYNGNFKELMQLKENNHAPAILYIYEDVVRN
                     IIYFGTTRGLFAFNNSDGTISKYNTEDKNILSIGKDKFNRLWICTSKKVFLFNGHEFE
                     DFNKNEISYDKGARCCTMDSRENMWLGNMDGLYLYSYQKCTRIFNTPIFFIKEYKKNF
                     IIAGTITGFYYINLDKFYLNKRPYYIFFDRFNGFSGIECGQNGTCMDSKGNVWIPTSD
                     RVVKFAPYRLKENTFAPRIKVYSFEISGKDLKWESILNEYSDIKKTIELTYDKHNIKI
                     SYMGISLSCPEKVKFKTRLVGYENSWSEPSGDLMAVYTNLDPGKYIFEIEACNSDGIW
                     TEAPLQIKFTIHPAFWQTWWFYFGIALIILALILLAFRIRLKRLSKKARLKQKMAQLE
                     MDLLHMQIEPHFIGNSMVMIKDLIYQKKYENALEAVDRFGEMLKFVAETTRERFITLE
                     KELLILKNYINFQKLKPENTIEFIINIAPNINVSEILIPPILLQPFVENSIAHGLRHK
                     KGAGQIQIDINNDLENSDYIIISIIDNGIGRKNVIKFETDKVKHKSVGIQNSTERLKN
                     FNNSSYKDIAIIDKEVGTEVKIWLKKEA"
     gene            9983..10810
                     /locus_tag="PKH58_05140"
     CDS             9983..10810
                     /locus_tag="PKH58_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX88444.1"
                     /translation="MKTIIIEDEQNAANFLLNALGRINATIAIKGIFNDPIEAIEGIR
                     TIKPELIFLDIDLNHEINGFGILDRVKDCDLNVIFTTGYREHALAAFEYNAVRFLLKP
                     YSDEKLKEAIERAKLITLGEQRQSIENIRMTESFLSKQTDRFPVRKASGDIVIVNVND
                     CVFLKPDGKSVILYFTDGTEEEAEFRPFADYAAILQNRGFIRIHQKFLVNILCIKKYV
                     NRNKAEITASVQELDSKNTAKEVKVMGDGGAVILSNGYHLAVSRIYSRTLKDALNIF"
     gene            10941..14738
                     /locus_tag="PKH58_05145"
     CDS             10941..14738
                     /locus_tag="PKH58_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX88445.1"
                     /translation="MKKFAFLLFLLAININIVIGSPIDTTLAKVVAENFYFQNNPTQM
                     SIINNTIEINLVYTRTEGGSDIYYVYNVSNNSGWIIVAGDNAVLPILAYSDEGSYSIA
                     NQPPAFVEWMNAYKDEILYVKQNSINADITITNKWNQLISNTINQSILLVSVSPLLST
                     TWAQDCYYNAQCPYDGSGDCNHALTGCVATAMAQILKYWEFPTTGIGSHSYTHSTYGT
                     LSANFGTTTYNWGSMPNNVTSTNSAVATLMYHCGVSVEMDYGPTASGATTSDVVDALI
                     DYFGYQNSAEYRCKVNYTSTSWKNLLKQELDESRPIQYRGSGSGGHSFVCDGYDNSDY
                     FHFNWGWSGSYNGYFAIGDLTPASQDFNTNNCAIVGIMPGSPGGDDFSVQNCSVTPGS
                     VYDGATIDVSSEQCYSGSTLDANMGSSYLGYYLSTNTSFSSSVDTYLGYDASGLGSDD
                     PCNSESETLTIPAGTLAGTYYILFVADYDDTFSETDENNNVCYYQLNVTNNIPTYVIN
                     TSSNPVAGGSTSGSGTYASGYNLILGATANNGYSFVDWTENGMYITDIPFFPLTVSSN
                     RTFVANFTQNPVNYTIATSSNPSAGGSTSGGGTYVSGSSCTVTATANSGYTFTNWTES
                     GSTISTNASYSFIVSVNRGLVANFTQSGQTIDLNNGLVAYYPFNGNANDASGNGNHGT
                     VNGASLTTDRFCNANSAYNFAGISNPQTISVANSSTLQFSNAATFSFWISMNSYQGMD
                     GWGSTTANGYHVIYAKDYDQCCITGGIGGLSNGEFTAHCGTGPGGIGLGDTVSNSAIN
                     QWINITYVITTTNVSIYANGNLIASQTGSMSFANSNTKNLWFGRLSSYWYPFNGKMDD
                     IRIYNRALNLAEIQAIHNLGSGIPSNGLVAQYPFNGNANDESGNGNNGIIQGNVISTS
                     DRLGNANSAYEFPGVAFNYIQVPDAASLHLNVFSLSAWIYSSTDYGSGQVIQKNRDVD
                     NGHYGLYTTSIGATNLYGGINNTTAISQPTIGTWHMVSGTVTGSTAKFYLDGQFVKDT
                     TLSNPYVYSGSEPLAIGMHYFSGVPSIWTYPFKGKIDDILIYNRVLSDAEVLQIYQSS
                     NSVIGLPSAAGTIAGTATVCQGQNSVTYTVPLITNATSYVWTLPGGATGTSTTNSITV
                     NYGASATSGNITVMGINPCGNGTASTIAITVTVCTNVQDNETSHKVNIYPNPVSGELT
                     IEIEENKDNFDFEIYNSIGQLVYKGNLIEKTVVQTSSFAPGVFLIKLENGKMFEVQKI
                     IKE"
     gene            14802..>18603
                     /locus_tag="PKH58_05150"
     CDS             14802..>18603
                     /locus_tag="PKH58_05150"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88446.1"
                     /translation="MKKLLLFVAVLMIVFVANSQTKKDNLLYQIKWNENIVQKKNYSS
                     IATSITSYCFLDDNTIAMLVADEQKIITYSISRNSVLKTIDIPDGGLDFDYYQDKFYV
                     LDNNELYIINNAGQIEQEISYRQPESRPFAVEKFRVIDGKKIINIADGTAWELSENGL
                     VLLDDYYWQYANGFKGRTEKQGFRSFELSINNSNESIKKTIQISSFGLTDDLATVRIM
                     SIDNNQITLDIETSTDNENEQPERFIIITDLQGSFISKIEIPFVYSTYIQSPFVKYKT
                     GLVYCLTALDGLYFYEINTEKNSISLPVGCPLKYHFNDFIEIIDTDELPYQNKNLNKS
                     MLSGSNCVTRTQVYRNALNFRDLTWTATSSNIQSSCGTISGLYFKTPTWVTVGSQTSV
                     PYKWGGWTDWDAFSDYADQGKYAGNYATTDASSPCPQNPYASSSDTYVIGDDCSGFVS
                     RCWETSSKYSTTGLPGISTNLGSATTSSIFSQLQPGDAINKSGSHVRLCLSQNPSGTG
                     SFMEASAVDWKVNNRSYSYTALTGYNCLKYNDINDARLNLAQAISISPTTVVQLQPLT
                     VTYKIINAGSESWSGYVRLYIIQSDGNEMSLQTSSLITLSAGASSTTFSFTSSSVSSP
                     TGTTQLEVRVKNSNTCDYQRYYKVGQGSWTNPLIFTINPPTAIDPCTSITSIGNCGSG
                     YSKTYTGGGSGAWFTSTNNPCGYTTPGIEKIYSFVAPTTGTYSIEVTAASGYVDYLWK
                     SGSCSSSGWNCIQDISSTGTFGSMSWTAGTTYYILLDDENSTTGTHTFYINCPCTVPG
                     SVSVSGGGTYCNSTTLNASGGSGGTIYWQGTTSGGTSTSTASTSQTVTSSGTYYFRAY
                     NSCGWGTQGSATVTINTTPASVSVSGGGTYCNSATLTASGGSGGTIYWQGTTSGGTST
                     STASNSQTVSSSGTYYFRANNACGWGAQGSATVTINTIPSSVSVSGGGTYCNSATLTA
                     SGGSGGTIYWQGTTSGGTSTANPGNTAVPTSSGTYYFRANNACGWGTQGNATVTINTT
                     PTQPSAISGTSPVCQETSGNYYSVTNVSGVTYTWNVPGGSIISGQGTNTINVYWTTSG
                     TNVITVTPSNSCGNGTSRSYSVNVLPLPSTVSVTGGGTQCNSATLTASASVGTIYWQG
                     TTSNGTSTSTPSTSQTVTSSGTYYFRAYNACGWGAQGSATVTINTTPASVSVSGGGTF
                     CNSATLTASGGSGGTIYWQGTTSGGTSTSTASSSQTVTSSGTYYVRSYNSCGWGTQGS
                     ATVTI"
BASE COUNT         6005 a         3411 c         3731 g         5456 t
ORIGIN      
        1 gagtggcaca aacactgcgc atcacgggcg attatcctca gggaaattat aaatttaccg
       61 gcagtgtaac tgatgtcaat ggttgcgtgt cttctttctt tgatgtgttc ctcgaaatga
      121 atacgatgcc tgcaccaccc actattggca acattacaca accaacttgt gctcttgcta
      181 cgggaagtat tgcattaaca ggattgcctt caggcaactg gaccattaat ccgggcaata
      241 ttaccggaac aggcgcaagc tacaccgtta gcggccttgc agccggaacc tataactaca
      301 ccgtgaacaa cggtcagtgt acttccgtcg catctactga tgttgtaatc aatacccagc
      361 ctgtaacgcc tgctgcacct gttgttggca ccatcacaca acctacctgt gttcttgcca
      421 ccggaagcgt ggtgttaaac ggattgcctt cgggaaactg gacgatcaat ccgggcggca
      481 ttacaggttc aggaatctca acaactcttt cagggcttgc tacaggaact tataatttta
      541 cggtaaataa cggccaatgt acttctgtgg ccactccaga tgttgtgatc aatacacagc
      601 ctgttacacc ggcagctcct atggttggaa ccatcacaca acctacctgt attcttgcga
      661 ctggaagcgt ggtgttaaac ggactgcctt caggaaactg gaccattaat ccgggcaata
      721 ttaccggaac aggtgcaagc tataccgtta gcggccttgc agccggaacc tataactaca
      781 ccgtgaacaa cggtcagtgt acttccgtcg catctactga tgttgtaatc aatacccagc
      841 ctgtaacgcc tgctgcgcct gttgttggca ccatcacaca acctacctgc gctgtacaat
      901 ccggaagtgt ggttttggac ggattacctt cagggaactg gacaataaac cccggtaatg
      961 ttagcggatc aacagtaagt acgaccattt caggccttgc tcctggaacc tataactttg
     1021 atgttaccaa tgcttcggga tgcacctcag cagcttcggc caatgtggat ataagtgcga
     1081 atttaattac taccgcagaa gcaggaccaa cagtaatata ttatggtact cctgttcaga
     1141 tcggagttgt taacaacggg tcgggaacta tcatctggtc acctgccacc gggttaaacg
     1201 atcctggtat cgcccagccc ttagcatcac cggcaacgac cactacctac actataactg
     1261 tcgataataa tggttgtgta cgaacggata cggtaacagt ggtttatggc ggaatagggc
     1321 atatgatcgc cggtaaaaca cgttatctga aaatggcaac cgcaggcaac ccggcgccaa
     1381 atccgcctac atacagtcct gcaatataca atatcgataa tgtagaagtg atcctgaaga
     1441 gtagtccggg aggagcggta atcgccacta cggttagtaa tgctaacggg gaatatcaat
     1501 ttaccggtgt cccggatgga gactatatac tggcttacga tcacattcca tatccggata
     1561 ctatgcaata tgttaatcac acgaatgctg ttgacttagc cttactgaaa tattatgtag
     1621 gccacaaccc attgactgat ccgtcgagga atttttcaga gaaacacagg aaagccgcga
     1681 atgtagataa taatgcgtca attaatactg ttgacgtggc gagaatcagc ggaaagatcg
     1741 ggatgccaaa cgatccggcc aaaaacttcc cgaaaggaaa ctgggtggca tttgatactt
     1801 tgataacgat ggctggttca gacttaaaca ttaccctgca aacggttgcc tatggagatt
     1861 acaatgcttc cagtgtaaaa taccagggag cagcaacaaa ctggggtatg gtaaaagaat
     1921 tgcctgatga aaatatcatc gtccggtcag acgaatccat catgatgagt gaccctgact
     1981 attttgaagt tcccttacgg attagtacaa aaatgaatga gctttccgca gtgggcctgg
     2041 agttgagcta tccaagtgat aagtacaaac tagtcagcgc ctccatgtca aatacgggca
     2101 aaaaaggcga tgcaataaag atcaatccta ctttggaaga gatcattgcc gctaataatg
     2161 acttgctcgt gacagatata gatggagtga tcagagtagt atttgccacc accgatcatt
     2221 ttgatattgc tgcaaatgaa gaactggtta agttaggttt ccgttcactc attgatccgg
     2281 gccgcggaga gcttgatttt aatttgaacg gtacgggttt gattgcaaat caatatggcg
     2341 aaattaatgc agatgcttac cttacgatgc caaagatctt cgtacagggt gatgatatgg
     2401 aagccggatt tgaattggca ggctatccca atccattcag cggcgatgct actttgacat
     2461 atagcattcc tgaaaacgga acggtaaaac tgagtgtata taatgctatc ggcgaactgg
     2521 tggctttact tgtaaacgaa tcgcagatca gtggtaaaca ttcagcagta ttttcgcctg
     2581 tgaatctgcc ggcgggcatg tacactttta aactggaatt tacaggttcg gataaatcaa
     2641 aacttatggt attgaagatg gttcattgat cctgtaataa atccgatttt cattagaaag
     2701 aggctgcctc atcatcgggg cggctttttt tttcatccta tgacaccaaa aaaaaggctt
     2761 taaaaatttt ttttgtataa aacgacaagc agataaaata aaatattata cttgtacatt
     2821 atttatattt ataaaaattt atgaaaaaac tattcgtatt tattgtatta ttcattgctt
     2881 taatcttgag ctcaaatgcg caaaattatt taaccaacac cgctgcacaa cctaatcatt
     2941 caataccaaa aggacctgtt gtgcagacct ttgaaggaac atttcctgcc tttgactgga
     3001 gcttgacagc gggcagcggg aactgggtaa aatccagtga gagaaatcac acctcgggtg
     3061 gatcattttc agcaatgtac aattgctggg gtattaacgg aaccgttcct gcttacctcc
     3121 agactcctga aatgattgtt actgcaggcg atgctacctt aacattctgg cgcaactatt
     3181 atttactcaa taatccaaat tatggacatg cagcagaatt atatattgac atatcaactg
     3241 atggcggttt aacttggaca tcaggtacta caaattattt agacacaata actttgaata
     3301 catggataca gtttaccatt gatctgactg ccaatatcgg gcaaaatgta attttaaggt
     3361 tcaaagctat ttcagacttt ggttcctata atattgccat tgatgatatt gcaggacctt
     3421 cacaaaattt accaccacac gatttggaaa ttcttgcttt aacaccatca tttgtactga
     3481 gtggcagtac tattgccccg gaagttgagg tgcgtaatat gggagatgcc actgaaacat
     3541 cattttctct gcatatatca gatggggggg cttataactg cacggtaccg gttactactt
     3601 ccatttctta tgggacaact tgtacagttt attgtccaga ctggactcca gcagacggaa
     3661 attatacttt gacagctaac gttattctcg ctcccgacac aaatacagat aatgatacca
     3721 taaagcaaaa tgttttagta tctccggaca gttggatatc ctggccggca ataaattcaa
     3781 acaggcttac aagttattgt gcatctgctg gttatacact gagttcggga atatatcgtt
     3841 ttttatattc ttttggcgga actggtggta acagtaagat ggttacaagc tacaatttag
     3901 aaacaggtac ttgtactttc ggtactgata tgcctgccta tagtaaaatg aacagcgctg
     3961 taacagtagg tgattttatg tatgtgaaca gaggtcgttg gggaactacg aattacaata
     4021 cattctataa atatgatgta ttaaacaaca cgtggagtac tgctgcaaat atgcctgccg
     4081 ccctggcata taatgctttt gctgctctta acggaaatat atactgtgtc ggaggtacta
     4141 ctacaggtgc tgttaatact gtgtatcgtt ataatattgc atcaaatacc tggacaacag
     4201 caagcccaat atctttggtc ggagggcttt atgccggcgg actggctatt gctgataata
     4261 aattagtata tatacagggt gttgttaacg gcgctttgac aggcgatgtt tttataggta
     4321 ctgttgacac gacaaattat gatgtaataa cgtggaccac aggaactccc tgtcctgttg
     4381 gtgtgttcgg aattaaagca gagccataca ccaatggcaa aatcatttat acgggtggtt
     4441 ctgacaactt aagcccattg catgttgtcc ctgattcata catttacgat gtgaattctg
     4501 atacatggac taggattcag gataaaatta ccccgacttt aggttatgct gctgctacat
     4561 ttaatatggg cggtaaaatg aggtactttg tttcagccgg acagagtgct tcaggctcac
     4621 ttttagataa ggtagaattt tttaatcatc agataattgc cgcagcatgc acaggcacac
     4681 cacaggcatc ggatataaca ggtcctgttt caatttgttc cggaactgaa gcaacgcttc
     4741 atttaagcag cacttatgca ctatcagaca tcacatacca atgggcatat tccacaaatc
     4801 tgggaggtcc ttataacaat ctcgggacat caagcgctca aagcacagga aatcttacat
     4861 caagcactta ctatatctgc acgattacat gtacgaacag tggcctgtca tacactaccg
     4921 cggaaaaaat ggttggagta tatcccttag ctacattgtc aatcattaca gatcatttat
     4981 gtgaaggaga ttcctcatat attttgttgt ctttagaagg caccggtcca tggttaatac
     5041 acggccttac tgaaattgca gcaagtggtt ctaccaatat ttttccagat attattgcaa
     5101 gtcaaagccc ttatcttgta tcatttaaac ctaatgaaaa ttcatattat cacgttgaat
     5161 ccgtaacaga taatactact ggatgctata cggataccgc taactgggta atggtcactt
     5221 tagctcctgt gcctactgca aatgcaggga cagatgcaac atatacaagc acacctgtgc
     5281 ttatcggcga tcctttaaat ggcccgggaa tcatcagttg gtctccttca gaagggttgg
     5341 ataatccgac catcgcacag ccatcagcat cccccttgct taccactacc tacaacttaa
     5401 caatcaacaa taatggatgt atacgtaccg atgccgttac ggttgcttct ggaggaacag
     5461 ggcatgccat cagtggaaaa acacgttatc tgaaaaaggc aaactcagga aatccggtgc
     5521 caacaccgcc ctcctacaat gctgtaatat ataatatagt taatgtagaa gtgaaactaa
     5581 agactagtcc cggaggagtc ttactttcaa ccactatgag tgatgctaac ggagtttatc
     5641 aatttaccaa tgtcccggat ggaaactaca tattagctta cgatcacatc ccttatgaca
     5701 ctatgcaatt cgttaatcag gttaatgctg ttgacctggc tttactgaaa tatcttatag
     5761 gccacgatac agtaattgat ccgtccagaa gttttacggc aaaacataaa aaagctgcaa
     5821 acgttgataa taatacaact attaatacta ttgacattgc aagaattgct tcaaaggttg
     5881 ggttgccata tactcccgcc aggaactttc caagaggaaa ctgggtaacc cttgacacat
     5941 ccgtgacggt tgccggttcc aatctgaaca tcaccctgca aacggtctcc tacggcgatt
     6001 acgatgcctc cagctccaga tacaaagatt cagcaagcac atggggtatg gcaaaatcat
     6061 tacctgatga aaatatcatc atcctgtcgg acgcatccat catgataaat gatcctgaat
     6121 attttgaagt tcccttacgg attagtacaa aaatgaatga gctttccgcg gtgggcctgg
     6181 agttgagtta tccaagtgat aaatacaaac tggtaagcgc ctctatgtcc aataccggca
     6241 agaaaagcga tgcaataaag atcaatccta ctttggaaga gatcattgcc gctaataacg
     6301 acttgctcgt gacagatgta gatggagtga tcagagtagt atatgccacc acatatcatt
     6361 ttgaaattgc tgccaatgtt gagctaatca gcttaggctt ccgttcgctc aataatccgg
     6421 gtaggggaga gcttgatttt aatctgagcg gtacaggtat gattgccaat caatatggcg
     6481 aaataaatga agatgcatac ctcaccatgc caaaaatctt tgtccagggt gatgatatgg
     6541 aagccggatt tgagtttgca ggcaatccca atccattcag cggtgatgct actttgacat
     6601 ataacattcc tgaaaacggt acggtaaaac tgagcgtata taatgctatc ggcgaactgg
     6661 tgactttact tgtaaacgaa tcgcagagca gcggaacaca ttcggcagta ttttcacctt
     6721 tgaatctgcc ggcgggcatg tacactttta aattggaatt tacaggttcg aataagtcaa
     6781 aatgtctggt attgaagatg attcattgat tcttgcttta gaattactca aatcacacaa
     6841 ataaaaaaac ccgcaatttg cgattttttt ttggttcctc atcaattgtc cctggtgttc
     6901 acttgtgtga atattaataa tacgattgaa aaatcgtgta atttatatta aaaacatata
     6961 cttttgttta aaacaaaacc ctcatgaata atcgctcaaa tgagttgtta aaaatatgca
     7021 cgctgcttca gatgggatta agtcgtatat gccattggtt gaaggattcc aaagggattt
     7081 ttattatgtt tatttttctg ttacttggca tttttacatt tgcccaaagt tatagttaca
     7141 aacattatac aatacttgat gggcttgttc aaaatcaggt gacgacgctc taccaggatt
     7201 accagggatt tatatgggtt ggaacaaaag ccggcgccag cagatttgac ggcattactt
     7261 ttaaaaatta cacacaacaa gaaggacttg cccctggact tgttacaggc ttttttgaag
     7321 atgaagaaaa aaatcttttt tgtatcacag ataatggtta ttcaatttta caaaatggta
     7381 attttaaaat agttcatata tttaatgaca aagaaattaa atattgtcat gggccgtttt
     7441 gcacaaaaga caaaacttgc tttatccttg cttttggtta tgaattatac aaatgcaata
     7501 aatcaaaatt tgaattatat tataggtttg aattaaaaac tgaaattaat gaatattacc
     7561 aaaacaatga aggccgggaa tttgtaaata cagattccgg tttttatgaa atatataaaa
     7621 atagacaatt ttcgaaattg ttctatcaaa aacccatcgg gttaaaaaat tgtaaaaatg
     7681 gtagttatat tgtggttgat ggtgataata ttaaaaataa atatccgaga ggtgtttatt
     7741 ttttaaataa taacttgacc actaaaattt ggaaagatac aagtaaaagc ttatgtaatg
     7801 ccttttcagt gcttaatgac acgaatattt taatagcatc taattttgat gaatggttga
     7861 tacttgataa aaatggacat aaaatagacg gagataaaaa taaggacttt gtgttaatta
     7921 cttctttttt ggaggataaa gagggaaata tctggatagg tgatgagagc ggactgtttc
     7981 gtttacaatc attcgctttt cgtaattata atgaggcttc agggcttgaa aagtacatct
     8041 ggactatact tgaagcgccg gattcgacga tattttttgc atcatttggt ggtaaattaa
     8101 tgaaattaaa agatgataag atagaaagag taattggata tgaaagattc ttaaattccg
     8161 gtactcaatt ttatatggga ggcacaagaa ctgctaatgg acatttgcta tttccagtta
     8221 ataatggaac tattttagat tattataacg ggaacttcaa ggagctcatg caactaaaag
     8281 aaaataatca tgcacctgcc attttatata tttatgagga tgtggtgcgc aatataatat
     8341 attttgggac aacaagaggc ctttttgctt ttaacaattc tgatggaacg atcagtaaat
     8401 ataatactga agataaaaat atcctttcaa tcggaaaaga taaattcaac agattgtgga
     8461 tttgtacttc aaaaaaagtt tttcttttta atggacatga gtttgaggat tttaataaaa
     8521 atgaaattag ttatgacaag ggagcaaggt gttgtacaat ggattccaga gaaaatatgt
     8581 ggcttggaaa tatggatggt ttatacttgt actcatatca aaaatgcaca agaattttta
     8641 acacaccgat tttctttata aaagaatata aaaagaattt cattattgct ggaactataa
     8701 caggttttta ctatatcaat ttagataaat tctatttaaa taaaagacca tattatatat
     8761 tctttgaccg gtttaacgga ttttctggta ttgaatgtgg acaaaacggt acatgcatgg
     8821 attcaaaagg gaatgtttgg attccgacat ctgatcgggt agtaaaattt gcgccttacc
     8881 gacttaaaga aaatacattt gcccccagaa taaaagttta ttccttcgaa atctcaggca
     8941 aagatttaaa atgggaaagt attcttaacg agtactctga cataaaaaaa acaattgagc
     9001 tgacttacga taaacacaat attaaaatat cgtatatggg gatttcgctt tcctgccccg
     9061 aaaaagtaaa atttaaaacc cgtttggtgg gttacgaaaa ctcctggtcc gagccatcag
     9121 gtgatctaat ggctgtttat accaaccttg atccaggtaa gtatattttt gagattgaag
     9181 cctgcaattc tgatggaata tggacagagg caccccttca aataaaattt acaattcatc
     9241 ctgctttttg gcaaacctgg tggttttatt ttggtattgc actgattatt ttagctttaa
     9301 tattattggc attccgcatc cgcctgaaaa ggttaagtaa aaaagctagg ttgaaacaaa
     9361 aaatggcgca gcttgaaatg gatttacttc acatgcaaat cgaaccacat tttataggaa
     9421 atagtatggt gatgataaaa gatcttattt atcaaaagaa atatgaaaat gcacttgaag
     9481 cagttgacag attcggcgaa atgctgaagt ttgttgccga aactactcga gaaagattta
     9541 tcactttaga gaaagaactc ttgatactca aaaattacat caattttcag aaactaaaac
     9601 ccgaaaatac aattgagttt attattaata ttgcaccaaa tattaatgtt tcagaaatcc
     9661 ttattccccc catcctttta caaccttttg tcgaaaattc gattgctcat ggattaagac
     9721 ataaaaaagg agctggtcaa atacagattg atattaataa tgatttggaa aatagtgatt
     9781 atataataat ctccataatt gacaatggaa ttggaaggaa gaatgtaatt aaattcgaga
     9841 cggacaaggt caaacataaa tccgtaggaa ttcagaattc gacagaacga ctcaaaaatt
     9901 ttaataactc atcatataag gatattgcaa ttattgacaa ggaggttgga actgaagtga
     9961 aaatctggtt aaaaaaagaa gcatgaaaac aattattatt gaggatgaac agaatgcagc
    10021 taactttttg cttaatgctc ttggtcgcat caatgcaact attgctatca aaggaatttt
    10081 taatgaccca atagaggcca ttgaaggcat cagaaccata aaacctgaat tgatttttct
    10141 tgatattgac ttaaatcacg aaatcaatgg atttggtatt ttagacaggg tgaaagactg
    10201 tgatttgaac gtaatattta ctaccggata tcgcgaacat gccttggccg ctttcgaata
    10261 taatgcggtg aggtttctgc taaaaccata tagcgatgaa aagctaaaag aggctattga
    10321 acgggctaaa ttaataaccc taggcgaaca aaggcaaagt attgaaaaca tcaggatgac
    10381 cgaaagtttt ttaagcaaac aaaccgatag gttccctgtt cgtaaagcct ctggtgatat
    10441 tgtaattgta aatgtgaatg attgcgtttt tctgaaacct gatgggaaat cagttatact
    10501 gtattttaca gacggcacag aagaagaagc cgaattcagg ccctttgcag attatgcggc
    10561 aatattacaa aaccgtggat ttatcagaat tcatcagaaa tttctcgtaa atatcctatg
    10621 tataaaaaaa tacgtgaacc gaaacaaagc tgaaattacc gcgtcggttc aggaattgga
    10681 cagtaaaaat acagccaaag aagtcaaggt gatgggcgat gggggggctg taatcctatc
    10741 gaacggatat caccttgcag tttcacgaat atattcccgt accttaaaag atgctttaaa
    10801 cattttctga tcttattctt attcgtttta taccactttt ctggttgatt ctaccaaaaa
    10861 gcagttattt tttgcaggta attcgatttt cataaaaaca acattacttt tacctttatt
    10921 aacatttaaa ttcaaatcct atgaaaaaat ttgcattttt gttatttctg ttagcgatta
    10981 atattaatat agtcattggc agtcctattg atacgacatt ggcaaaagtt gtcgctgaaa
    11041 atttttattt tcaaaataat cctacacaga tgtcaattat taataatacc attgaaataa
    11101 atttagttta tactagaacg gaaggcgggt ccgacattta ttatgtttac aatgtgagta
    11161 ataatagtgg atggataata gtggcaggtg ataacgctgt tttaccaata ttagcctatt
    11221 ctgatgaagg cagttattct attgctaatc agccacccgc atttgttgaa tggatgaatg
    11281 cctacaaaga tgagattctt tatgtaaaac aaaacagcat caatgcagat attacaatta
    11341 ctaacaaatg gaatcaatta atatcaaaca ctattaatca aagtattctc cttgttagtg
    11401 tttctccttt attatcgact acctgggctc aggattgtta ttataatgca caatgtccgt
    11461 atgatggatc cggggattgt aaccatgcgc tgacagggtg tgttgcaaca gctatggcgc
    11521 agatattgaa atactgggaa tttcctacaa caggaattgg tagtcattct tatactcatt
    11581 ctacttacgg gactcttagt gcgaattttg gaactacaac ttataactgg ggttcaatgc
    11641 caaataatgt tacatctact aattcagccg ttgccaccct tatgtatcat tgcggggtca
    11701 gtgttgaaat ggattatggc ccaacagctt caggggcaac aacatctgat gtagtcgatg
    11761 cgctaattga ttattttgga tatcaaaatt ccgcagaata tcggtgtaaa gttaattaca
    11821 cctctacaag ctggaagaat ttactgaaac aagagttaga cgaatcaaga ccaatacaat
    11881 acagaggaag tggtagcgga ggacattcat ttgtgtgcga cggctatgac aactccgatt
    11941 attttcattt taactgggga tggagcggaa gttataatgg atattttgct atcggggatt
    12001 taacacctgc cagtcaagac tttaatacta ataattgcgc catagttgga atcatgcccg
    12061 gttcaccggg aggtgatgat tttagtgttc agaattgttc agttactcca ggttcagttt
    12121 atgatggtgc aactattgat gtcagtagtg agcaatgcta ttccggctca acgcttgatg
    12181 ccaatatggg aagttcatat ttaggttatt atttatcaac aaatacgtca ttcagctctt
    12241 ctgttgatac ttatttaggt tatgatgcct ctgggctggg aagcgatgac ccgtgtaatt
    12301 cagaatcgga aacattgaca ataccagccg gcacattagc aggaacatat tatattttgt
    12361 ttgttgcaga ttatgatgat actttttcag aaactgacga aaacaataat gtttgttatt
    12421 accaattaaa tgtgaccaat aatatcccga catatgtaat taatacctca tcaaatcctg
    12481 tagcaggtgg ctcaacaagc gggagcggga catatgcaag tggttataac ttaatattag
    12541 gtgcaaccgc aaataatggt tattcctttg ttgattggac tgaaaatgga atgtacatta
    12601 ctgatatacc attttttcca ttgacagttt caagtaacag aacattcgtt gccaacttta
    12661 cacaaaatcc agttaactat acaatagcga catcatccaa tccttcagca ggaggttcga
    12721 caagtggagg aggtacttat gtgagtggca gttcttgtac ggtaacagca actgccaaca
    12781 gcggctatac ttttactaac tggacagaaa gcggttctac aatttcaact aatgccagtt
    12841 attcattcat cgtttcggtt aatagaggtc ttgttgccaa ctttacgcaa agcggccaaa
    12901 cgattgattt aaataatggc ttagttgctt attacccatt taatggaaat gccaacgatg
    12961 caagtggcaa tggaaaccat ggcacagtga atggtgcaag cttaactact gacaggtttt
    13021 gcaacgcaaa cagtgcttat aattttgctg gaatttcaaa tccacagaca atttcagttg
    13081 ctaatagttc aactcttcaa ttttcaaatg ctgcaacttt ttcattctgg ataagcatga
    13141 atagctacca aggcatggat ggctggggtt caactaccgc aaatggttat catgtaatat
    13201 atgcaaaaga ttatgatcaa tgctgtatta caggtggcat aggtggtttg tcaaatgggg
    13261 aatttactgc tcattgtgga actggtccgg gtggtatcgg cttaggcgat actgtatcaa
    13321 attcggcaat taatcaatgg ataaatatta cctatgtaat aactacaacc aatgttagca
    13381 tctatgcaaa tggtaacttg atcgccagtc aaaccgggtc aatgtccttt gcaaattcaa
    13441 atacaaaaaa cctatggttt ggtcgattgt catcctattg gtaccctttt aatggcaaaa
    13501 tggatgatat aagaatttat aatcgcgcct taaatttagc agaaattcag gcaatccata
    13561 atttaggttc ggggattcca tctaatggtt tagtagctca gtatccattt aacggtaatg
    13621 caaatgatga aagtggaaat gggaataatg gaatcattca aggtaatgta atatcgactt
    13681 ctgataggtt gggtaatgct aattcggcct atgagtttcc tggtgttgca tttaattaca
    13741 ttcaggtacc agacgcagct tcacttcatc tgaatgtatt tagtttaagc gcatggatat
    13801 attcatcaac tgattatggt tccggccagg tcatccaaaa aaacagagat gtagataacg
    13861 gacattatgg cctatataca acaagtattg gggctactaa tttatatgga ggaataaata
    13921 atactacagc tatttcacaa ccaacgattg gaacgtggca catggtctca ggaactgtga
    13981 ctggttcaac agcaaaattc taccttgacg ggcaatttgt caaagatact acgttatcaa
    14041 atccatatgt ttactctggt tctgagcccc ttgctattgg catgcattac ttctcaggcg
    14101 tgccaagtat atggacttat ccttttaaag gtaaaataga tgacatttta atatacaaca
    14161 gagtgctttc tgatgcggaa gttttacaaa tctatcaatc atcaaatagt gttattgggt
    14221 tgccatcagc agcaggaaca atcgctggaa cagcaactgt ttgtcagggg caaaactctg
    14281 taacttatac agtgcctctt atcactaatg ctacatccta tgtctggacg ttaccaggtg
    14341 gtgcaaccgg cacaagtaca accaacagca ttacggtaaa ctacggcgct tccgcaactt
    14401 ctggcaatat tacggtaatg ggaattaacc cttgtggaaa tggaacggct tcaacaatag
    14461 caattactgt gactgtttgt acaaatgtac aggataatga aacaagccac aaagtaaata
    14521 tttatccaaa tcctgtttca ggcgaactaa caattgaaat tgaggaaaat aaagataatt
    14581 tcgattttga aatttataat tcaattggac aacttgttta taaaggaaac ctgatagaaa
    14641 aaacagttgt tcaaacaagc agttttgctc ccggagtatt tttaataaaa cttgaaaatg
    14701 ggaagatgtt tgaggttcag aaaattataa aagaataata atgacaaatt cagataccga
    14761 aacaataaat ataggatgaa taaactttaa aatactattt tatgaaaaaa ttattacttt
    14821 ttgtagcagt actgatgata gtttttgtgg caaattcaca aacgaaaaaa gacaatctac
    14881 tttatcaaat taaatggaat gagaatattg ttcaaaagaa gaactatagc agcattgcaa
    14941 cttcaataac atcttattgt tttttagatg ataatacaat tgctatgctt gttgcagatg
    15001 aacaaaaaat cattacatat tcaataagca gaaacagtgt gcttaagacg atagatatac
    15061 ctgatggcgg attagatttt gattattatc aagataaatt ctatgttttg gataataacg
    15121 aactatatat tattaataat gcaggacaga ttgaacaaga aattagctac agacaacctg
    15181 aaagcaggcc ctttgcagta gaaaagttta gagttattga tgggaaaaaa attattaata
    15241 ttgctgacgg cacagcttgg gagttgagcg aaaatggatt agtgctttta gatgattatt
    15301 attggcagta tgcaaatgga tttaaaggaa gaacggaaaa acaaggattt agaagttttg
    15361 aactctcaat aaataacagt aacgaatcaa taaagaaaac aattcagata agcagctttg
    15421 gattaactga tgatttggca actgtaagaa taatgtcaat tgacaacaat cagattacac
    15481 ttgatattga aacatcaacg gataatgaaa atgaacaacc tgaaagattt ataattatta
    15541 ctgatttaca aggaagtttt atttcaaaaa ttgaaatacc ttttgtttat agtacttata
    15601 ttcaatctcc ttttgtaaaa tataaaacag gattggttta ttgtcttaca gcacttgatg
    15661 gattatattt ttacgaaatt aatacagaaa aaaactccat tagcttgccc gtgggatgcc
    15721 ctttaaaata tcattttaat gattttatag aaataattga taccgatgaa ttgccttatc
    15781 aaaataaaaa cctgaataaa tcaatgttat caggtagtaa ttgtgttacc agaacacaag
    15841 tatacaggaa tgctttaaat tttagagatt taacatggac agcaacatcc tcaaatattc
    15901 aaagctcttg tggaaccatt tctggtcttt attttaaaac gcctacttgg gtgactgtag
    15961 gcagtcaaac atcagtacct tataaatggg gaggatggac agattgggat gctttttcag
    16021 attatgccga tcaagggaaa tatgctggaa attatgcaac aactgatgca agttcaccgt
    16081 gtccacaaaa cccatatgct agcagtagtg atacatatgt tataggagat gattgtagcg
    16141 ggtttgtgag ccgttgctgg gaaacttcat caaaatacag cactactgga ttacctggta
    16201 tttcaactaa tttaggcagt gctacaacat caagtatatt tagtcaactt caacctggcg
    16261 atgctattaa taaatctgga agtcatgtca ggttatgttt atctcaaaat ccaagtggaa
    16321 ctggttcatt tatggaagca agtgctgttg attggaaggt taataataga tcttatagct
    16381 acaccgcact aactggttat aactgtttaa aatacaacga tattaatgat gctcgcttaa
    16441 atctggctca agcaatttca atttctccga ctactgttgt tcaattacaa cctttaactg
    16501 taacgtataa aattattaat gctggttcag aatcatggtc gggttatgtt cgcttatata
    16561 tcattcaatc agatggaaat gaaatgtctt tacaaacgtc aagcctaatt acattgtctg
    16621 ctggagcctc atcgaccaca ttttcgttca cttcatcatc agtttcttca ccaactggca
    16681 ccacacaatt agaggtcaga gtaaaaaatt caaatacgtg tgattatcaa agatattaca
    16741 aagttggaca agggtcatgg acaaatccat tgatatttac tattaatcca cctacagcta
    16801 ttgatccctg cactagtata acctctattg gcaattgcgg ttcgggttat tcaaaaacct
    16861 acactggcgg gggtagcggc gcttggttta ccagtactaa taatccatgt ggatatacaa
    16921 ctcctggcat cgaaaagatt tatagttttg tagcacccac aactggcaca tacagcattg
    16981 aagtcactgc tgcaagtggt tatgttgatt atttatggaa atccggttcc tgttcatcat
    17041 caggttggaa ttgtattcaa gatattagtt ctactggaac ttttggatca atgtcatgga
    17101 cagctggaac tacatattat atattacttg atgatgaaaa ttcaactacc ggtactcata
    17161 cattttatat aaattgccct tgtactgtac ctggttcagt atcagtaagc gggggcggaa
    17221 catactgcaa tagcacaaca cttaatgcaa gcggtggaag cggcggtaca atatactggc
    17281 aaggtaccac aagcggtgga acttcaactt cgactgcttc aacaagtcaa actgttacct
    17341 catcgggaac ttattatttt agggcttata atagttgtgg ctggggaacg caaggcagtg
    17401 caacagtaac tataaatacc acaccagcat cagtatcggt aagtggtggc ggaacatact
    17461 gtaatagtgc aacattaact gcaagcggcg gaagtggtgg cacgatatac tggcaaggga
    17521 caacaagtgg cggaacttcc acttctacag catcaaatag tcaaacagta agttcatccg
    17581 gcacttatta ttttagggct aataacgctt gtggatgggg agcacaaggc agtgcaacgg
    17641 taacaataaa cacaattccg tcatcagtat ctgtaagcgg tggggggaca tactgcaata
    17701 gcgctacatt aacggccagc ggaggaagtg gcgggacgat atactggcag gggactacaa
    17761 gtgggggaac atcgaccgca aaccctggaa atacagcggt acccacttca tcaggaacct
    17821 attatttcag ggctaataac gcttgtggtt ggggtacaca aggaaatgca acagtaacaa
    17881 taaataccac accaacccag ccaagcgcaa taagcggtac aagtccggta tgtcaggaaa
    17941 caagcggtaa ttattattct gtaacgaatg tatcaggagt aacttataca tggaatgtac
    18001 ctggcgggag tataataagt ggacaaggta caaacacaat caatgtttat tggacaacaa
    18061 gcggaacaaa tgtaataacg gttacaccta gcaatagttg tggtaatggt acttcaagaa
    18121 gttattccgt aaatgttttg cctttgcctt ccacagtatc tgtgacaggt ggaggtaccc
    18181 aatgcaatag cgcaacttta actgcaagtg caagtgttgg cactatatat tggcagggca
    18241 caacaagcaa tggaacttcc acatctactc cttctacaag ccaaactgta acttcttccg
    18301 gcacttatta tttcagggct tataacgctt gtggatgggg agctcaaggc agtgcaacgg
    18361 taaccattaa cactacacca gcatctgtat ccgttagcgg tggtggaaca ttctgcaaca
    18421 gtgctaccct cacggcaagc ggtggcagcg gtggtacaat atattggcaa ggaacaacaa
    18481 gtggagggac ttccacttct acagcttcct ccagccaaac cgtaacatct tcaggaactt
    18541 attatgtcag gtcatacaac agttgcggtt ggggaactca aggcagcgca acggtaacaa
    18601 taa
//
LOCUS       DAOPYB010000044        18149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3225_length_18149_cov_10.870896, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000044 DAOPYB010000000
VERSION     DAOPYB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18149
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3225_length_18149_cov_10.870896"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKH58_05155"
     CDS             <1..668
                     /locus_tag="PKH58_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX88447.1"
                     /translation="AVFNPFLYFLMENYGLMYTSPAIAAVIIGTIPVFAPITGYLAFK
                     EKLRWYNIAGILLSFTGVVMMLITPDFKISVNPAGLLFLAGAVMASLIYAVGLKKLTA
                     RYSSVTIISWQNLTGIFLFLPFFLIFEMKDAIRVPLNATIITSFLFLSILASSLSYVF
                     YVKAIKNLGIGKASIFTNLIPVFTAIASFFLLGELFTFQKIAGILIVIFGVVISQISS
                     REK"
     gene            761..2416
                     /locus_tag="PKH58_05160"
     CDS             761..2416
                     /locus_tag="PKH58_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNX88448.1"
                     /translation="MKYTIRQPKPADYIEYIVHHSLLGIRNSLFALLSLFFIIPLVNY
                     FFFPETGEHHFLLLFWAIIPIALLNLALFSFKKLRERHALYSTIINVLMAVAILLIGI
                     HAKADTPGTSFFSVMVVLIFMGTAAMYRIDFVNFLIADILLLASYITANIINGNFTNN
                     PVFMMNNFLFVLALSVLACIISWEISHLNYRNYIHLKSIEKKNQELQREITLRDEARQ
                     NLDASNKIASSVLNSIPDFIFVIDQNLRIVMVNEHIRTIMKQEGITGDPLGKQFNESS
                     KFLSPESVAGITDVFRSKKYSLATYHVEVNGNKYITEARVIPIIKNQVVEQIMIIIRD
                     VTKEREFEEVKLRDAEQKELLLREIHHRVKNNLAIVISMLSLQSRYNPDPVLTGIIQD
                     IELRIRSMALIHEHLYRSDTLDRIPLADYLKSLSAVIASTFQQPHINFETKLEPIDSD
                     IEAALPIGLIVNELLTNAFKYAFPDKRHGNILLELKQLNGEQVELRISDDGIGLPEGF
                     SMEEGSTMGVFMVRLLVEQLYGKLEITNEKGATFRLTYNHKII"
     assembly_gap    2491..2590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2591..2844)
                     /locus_tag="PKH58_05165"
     CDS             complement(<2591..2844)
                     /locus_tag="PKH58_05165"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNX88449.1"
                     /translation="MKKLAVVALGGNALLMSGQKGTIDEQEANAHATSECLVKLLKRD
                     YNIVITHGNGPQVGNIMLANSAGYKMFGIPEMPLDICVAYS"
     gene            complement(2911..>3962)
                     /locus_tag="PKH58_05170"
     CDS             complement(2911..>3962)
                     /locus_tag="PKH58_05170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNX88450.1"
                     /translation="MVNEEYCKMTGYSREEEIGTTWLRHIVPCDQERLREYNSHRFTD
                     PENAPDKYEFSFINKKGEIVNAMMSVAPVPGMRKIIASFTDISRIKQTEIRLQKLAAE
                     MTVKSENVNLLLSIIAHDLRNPFNAIIGLTSILLDNLPERSLIEINKFITLINTESKQ
                     AFFLLEDLLAWAKSQQDKVLFNPQPLNLPEVCRDVVCNISVYADEKQIAIDLNPEPDA
                     HPEPIIVTADKNMIKTVIRNLISNAVKFSIPGSAIHIQTESQDHEVSVSVIDHGVGIE
                     EKNMKKLWSISEKYTTPGTANERGNGLGLILCKEFVEKHGGRIWAESEPGNGSRFIFT
                     LPKNGDESCPEKPSR"
     assembly_gap    3963..4062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4063..4337)
                     /locus_tag="PKH58_05175"
     CDS             complement(<4063..4337)
                     /locus_tag="PKH58_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX88451.1"
                     /translation="MGSERIFVEEPAQRILALEKELQALKKENERLREVEQQFNAFTE
                     LVSKNQVEFKTIFDKNSSAIAILEPDTIITMVNEEYCKMTGYSREEE"
     gene            4489..5748
                     /gene="mtaB"
                     /locus_tag="PKH58_05180"
     CDS             4489..5748
                     /gene="mtaB"
                     /locus_tag="PKH58_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNX88452.1"
                     /translation="MPKKIAFHHFGCKVNFSEASALARQFRERGYELTGYREQADIYV
                     INTCAVTREAEKKCIYAIRQTHRLNPEAKIVVMGCFSELNPEEIRSIEGVTLVLGQSD
                     KFRLFDLLEDSEPATSPAACDFVPSYSMGDRTRSFLKIQDGCDYYCAYCTIPLARGHS
                     RSDTIANVVREARDIAAAGVREIVLTGVNIGEFGRHQQETFLDLLIRLEQEVDIPRIR
                     FSSIEPNLLSDEIIRLVASSRKIMPHFHIPLQSGSDKTLRAMHRKYDTALYAGRISTI
                     RSLLPYACIAADVIVGFPGETDADFDDAYRFMEGLDISYLHVFSYSRRDHTRAAAMED
                     QVPGHIIHLRSEELHRLSETKKHRFYEQNRGRREEALLESTNVDGMMHGFTGNYIKVK
                     HPFDPGLTNQIVPVILDRPDDDMTFII"
     gene            5759..>6128
                     /locus_tag="PKH58_05185"
     CDS             5759..>6128
                     /locus_tag="PKH58_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNX88453.1"
                     /translation="MNLETITRKTCDIVSLAAAFIREESTRLSASDIRAKSAHNYVTY
                     IDEQSEKLLIGELSKLVPGSGFIAEEDPSLQRKELNWVVDPLDGTTNFIHGLPMYSIS
                     VALMEGDEVIAGVIYEAGQQE"
     assembly_gap    6129..6228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6229..6699
                     /locus_tag="PKH58_05190"
     CDS             <6229..6699
                     /locus_tag="PKH58_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNX88454.1"
                     /translation="MEGDEVIAGVIYEAGQQEYFYTWKGAPSYLNQKAIRVSVTKKMD
                     DSLFATGFPYYDYSRLDKYMELFRFMMQHTRGIRRLGTAAADLAWTACGRFDGFYEYG
                     LSPWDVAAGTIIVANAGGTVCDFRSGNNFIFGKEIVATNGILHEELMKEIHHIF"
     gene            6762..>7714
                     /locus_tag="PKH58_05195"
     CDS             6762..>7714
                     /locus_tag="PKH58_05195"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNX88455.1"
                     /translation="MLTTEVLSGQNSRREINAVKAVSDIRIDGVLDEPAWADAPVASG
                     FTQYEPYNGKMPSFRTEVKFLYDNSGLYVAAMMYDPHPDSIPRQLGPRDSEWLNADDL
                     TVMISPFDDGINASVFQLYSSDVQTDYKLSFTNGADNTDRTWDAVWQSKSVINDSGWV
                     AEIRIPWSAIRFSEQGEMSWGINCFRNIRRYRENSSWNPVDNKIQGKANQEGLLKGIR
                     DIKPPLRLSLMPYLSGYLEKNPEVSDWQTSFNYGADLKYGISQSFTLDMTLIPDFGQV
                     QSDYKIYNFTPFEIQYDEKRQFFTEGTELFSKGGVFYSRRIG"
     assembly_gap    7715..7814
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7815..9029
                     /locus_tag="PKH58_05200"
     CDS             <7815..9029
                     /locus_tag="PKH58_05200"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNX88456.1"
                     /translation="VFSQALKNNSYISLLNTNVYTPETGYCADVAGTEFKFANKKYTY
                     GVWGTFLVSQKFSESTLPDRGYHYNLSVGKLSGNFQFTLSQLLETDKYDPNDLGYNQR
                     NNKFENGLDLYYNIYDPFWKVLNWSNYLYIGYDCMYEDLKYSSFYISAETMTNTRKYL
                     TLGSNMEIKPVDAHDYYESRVPGRVYIDPASTEVSFWFSTDYRKKFAFDGSVAGFYCP
                     EFSATGYAVSLGPRLQPNKRLTLICELSGEFLQNFNGYVMDSLDAMQQPVIIFGNRDV
                     NTITSTLNGTFMFSSKMSLNLQARHYWVTARYHSFSDLDESGHLQPNDYQGAHDLNFN
                     LLNIDLGFTWNFLPGSQLSLVWKNAITTQGDQVEDDFLRNFNQMIESPASNSFSVKVL
                     VYLDALYLKKKK"
     gene            9099..9171
                     /locus_tag="PKH58_05205"
     tRNA            9099..9171
                     /locus_tag="PKH58_05205"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9132..9134,aa:Gly,seq:ccc)
     gene            9941..10807
                     /locus_tag="PKH58_05210"
     CDS             9941..10807
                     /locus_tag="PKH58_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88457.1"
                     /translation="MLDIIIFNVELGQCIFFYPRPDKKEFGLMVDCGNTPNFEPIDKL
                     IEWNLLPKKSDERNLKYLLKSLTLTNYDQDHFSGLPYLQKKVEIETTRFPKNISSTDL
                     RKIKDEITEPIEAVIKIIDNAGKGITLDTPYEKRCFHLEQDDFPDEDIDTNKLSQIVF
                     VTFKDVRICIPGDLTSDAWEKILKKANVIDYLKGTKIFIASHHGREDGFNENVFKYCK
                     PEVIILSDKDIIHKTQEGQTQTYAGMVQGNGIILNGKTGQPRKVLTTRSDKHIWIRIE
                     DNGARTYKTITE"
     gene            10824..11324
                     /locus_tag="PKH58_05215"
     CDS             10824..11324
                     /locus_tag="PKH58_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88458.1"
                     /translation="MKDIIDKLSTYNIFNYFLPGILFVIIAKYFTDYDMILKNNLIGT
                     FLYYFIGMVISRFGSLVIEPILKKIRFVKFANYSDFISASKEDSKIELFSEINNTYRT
                     ITAMFFLLLLLKTYNYFDTQYHFAKGISFGILIILILLMFLFSYRKQTSYITKRITTA
                     KKSTSI"
     gene            11321..13237
                     /locus_tag="PKH58_05220"
     CDS             11321..13237
                     /locus_tag="PKH58_05220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNX88459.1"
                     /translation="MTELIWEGKYKDGKKVAPVRIALPFQTIETINESAQQRQKTLDF
                     FANEHTAQYNKEWRNRLIWGDKKYVLPSLLPEFAGKVNLIYIDPPFNVGSDFSFTATI
                     ADNPETEEDEATTFVKQPSIIEQKAYRDTWGKGLDSYMQWFYETVLLLRELLTADGSI
                     YVHLDWHVSQYCKVILDEVFGYENFRNEIVWQRTTNTGSSKGMANKLSTDTDSIFYYS
                     KNTKATFNKQYKPYSDEYLARFKFKDDKGMYRWQYMATYSEKKLKEFESKGMIRWQKG
                     SSNPEYKQYIDDLKGIPLNNLWTDIFHVNPMAIEKVNYETQKPEELLERIIKISSNDG
                     DLILDCFCGSGTTAATAEKNNRRWITCDLGRFAIHTARKRFLGIDNVKPFIVQNLGKY
                     ERQQWIKAEFENAENRLQQEKTYKLFINELYHAKSLNGYTWIHGAKAGRMIHVGSVET
                     PVTVEDIKATILEFWKLVGKEKSTTTNGIDFLGWDFAFDVNETAAQFATSNKVDVKFK
                     KIPREVLEKKAVEQGDIKFYELASLKVKTKIDKKKASITLDNFIIPPDDIPEDVREHI
                     THWSQWIDYWAVDWNYCDDTFHNEWQSYRTKQNSKIELTTGKIYDENGKYTVLVKVID
                     ILGNDTTKAIQIEI"
     gene            13240..13701
                     /gene="tnpA"
                     /locus_tag="PKH58_05225"
     CDS             13240..13701
                     /gene="tnpA"
                     /locus_tag="PKH58_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNX88460.1"
                     /translation="MAGTFSQIYIQVVFAVQNRESMIQSSWEEELYKYIAGIIRNKGQ
                     KLLAINGMPDHIHFLFGMKASCCLSDLVREIKKSSNDFIKDKKFSKPKFNWQEGYGAF
                     SYSHSQIDSVIKYIMKQKEHHKKNTFREEYLDFLKRFEIEFEEEYLYKWIE"
     gene            13830..16400
                     /locus_tag="PKH58_05230"
     CDS             13830..16400
                     /locus_tag="PKH58_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNX88461.1"
                     /translation="MVKRKKNNPSQKDMFNIQEQLSTAACVPAIREAVKGWRVDGYKG
                     ITGTTRELLNFWFRTDHVLQNGNSFRFHTAQREAIETLIYIFEVAKVKNRKELLEKFA
                     FTQKDLRLPPYDEFARYCLKMATGSGKTFVMCMSIVWQFANYLRGEKGYANNFLILAP
                     NVIVFDRLKTDFESGYVFRTLPLIPKHLLWLWDMDYYMRGDTERSHSQGAIYLTNIQQ
                     FYERGTRNNNEEPEVMTTMLGSIPPAQKTEITDFDERISKRDGLLMVINDEAHHTHEE
                     DSEWNNTIRKLHTQKPIAAQLDYSATPRYSKGSLFAWTIFDYPLKQAILDRIVKRPIK
                     GISKLEESKSTVASVRYSGFLTAGVERWREYSEQLKPLKKKPILFIMMNNTEEADDIG
                     DWIRTKYPKEFGGEKTLIIHTDKTGEVSKKDLDAARKTARDVDHDESPVNVIVSVLML
                     REGWDVQNVTVVVGLRPYSAKANILPEQTIGRGLRLMFRNMTDGYTERVDIIGNNAFM
                     EFVEDLEQLEDLKFETFQIGKDKLKILTVQPEEKKKELDISIPNISPLLSRKKSLAEE
                     ITAIDIMKFNINKLPLKGKEITDTKKFIYEGRDILTDEKLLEREYMIPQAQTAEEVIG
                     YYARRITQNIKLPSQFAVLAPKVRAFFERKAFGQTVDLNDPVVIKAISSNIASYVVIK
                     EFEKVLRELVVEQKEPQLLSENRFLSSTPPFPTSKKVLEAKKTVFNYTPCDNEYEYEF
                     AKFLDKSEDIISFAKLPEQFGFCIQYTDTLANIRNYFPDFIALHEDGSRWIIETKGRE
                     DVEVKLKDNAAINWCNTATELTKENWNYLKVLQKEFEVLHPDNFEELLAGVNPPTLFD
                     "
     gene            16412..17479
                     /locus_tag="PKH58_05235"
     CDS             16412..17479
                     /locus_tag="PKH58_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4917 family protein"
                     /protein_id="HNX88462.1"
                     /translation="MAKVEIPPYNELQSYADVVKRVKNRQIHLLLGNGFSMSYDNNIF
                     SYNALSKFIHVIKNKELQQLFDIVNTNNFELLMQQLDNASQIAEVFGADKKVVKKIRD
                     ASETLKTSLIEAIKELHPDHVFTIPENKSQACANFLNTFLSQGGGVFTTNYDLLLYWV
                     CERNQLENRVDGFGRDSEETDEWVEPDERQYSELRWGKYKEEQSIYYLHGALQIFDTG
                     IDIIKEEYTSEHYLLENIKRRMAKKEYPIFVTAGTGIDKLTHITHNKYLSFCYENLTA
                     IEGSLIVYGFGFGEYDDHIIKAINKAGKKRLDENEKWHMLKSVYIGVYSENDYEYIKK
                     IQKQFKVPIRLFDSKTVNIWG"
     gene            17642..>18149
                     /locus_tag="PKH58_05240"
     CDS             17642..>18149
                     /locus_tag="PKH58_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNX88463.1"
                     /translation="MDKNEFIRNFRIAFGNYELPIAIWYSEKAVAQEEKTRGCFIKYL
                     KPTREGKIVSLSLETISCPGAKLYCGFTGAAPFIPNFVSEKERYKKTPEMVTDFIADL
                     NMPDKSKQFLNFASIDRIENFDNIDALIFFATPDVLAGLVSWALYDTNEPDAVSVPFG
                     SGCSSMVSQ"
BASE COUNT         5414 a         3797 c         3829 g         4709 t
ORIGIN      
        1 cggctgtttt caatcctttt ctttattttc tgatggagaa ttacgggctg atgtatactt
       61 ccccggctat cgcggcggtc atcatcggca caatccctgt ttttgccccc attaccggct
      121 acctggcttt caaagagaaa ctgcggtggt ataacatcgc cgggatcctg ctctcgttta
      181 ccggcgttgt catgatgctg atcacgccag actttaagat cagtgtcaac ccggcaggtc
      241 tgttgttttt ggcaggagca gtcatggctt ccctgatata tgccgtgggg ttgaagaaac
      301 ttacagcacg gtacagttcc gtcaccatta tttcctggca aaacctgacg gggatttttc
      361 tgttcctccc ctttttcctg atctttgaga tgaaagatgc gatccgtgtc ccgttaaacg
      421 ccaccattat tacctccttt ttgtttctct cgatcctcgc ctcttcctta tcctacgtct
      481 tttatgtgaa agctattaaa aacctgggta tcggcaaagc aagtatcttc accaacctga
      541 taccggtgtt caccgccatt gcctcctttt ttctgttggg agaacttttt acctttcaaa
      601 aaatcgccgg tatactgatc gtcatcttcg gtgtggtcat ttcacagatc agctcccggg
      661 aaaaatagtc aactttaaat ggataataat tgaggtttgt ttttcatatc tttgttaaac
      721 caagatggat tataaaacat tctcgtatca ccatatcctt atgaagtata ctatacgaca
      781 acctaaaccg gcagattaca tcgagtacat cgtacatcat tctttattgg gaataagaaa
      841 cagtcttttt gccttattat cccttttctt tattattccc ctggtcaact atttcttttt
      901 tcctgaaacg ggagaacatc attttttact tcttttctgg gccatcatcc ccatcgccct
      961 gctgaacctg gcgttgtttt cctttaaaaa actccgtgaa cggcatgcgt tgtattctac
     1021 catcatcaat gtactgatgg cagtcgccat tttactgatt ggcattcatg ccaaagccga
     1081 tacgccgggg acttcctttt tctctgttat ggtggtactt attttcatgg ggacggccgc
     1141 catgtaccgg attgattttg tcaacttttt aattgccgac atcctcctgc tggcatcata
     1201 cattaccgcc aacatcatca acgggaattt caccaataac cccgttttca tgatgaacaa
     1261 cttcttgttt gtattggccc tgtcagttct tgcctgtatt atctcttggg aaatttcaca
     1321 tttaaactac cgtaattata tccacttgaa atctatcgaa aaaaagaatc aggagctgca
     1381 acgggagatc accctgcgtg atgaagcgcg tcagaatctc gatgcatcaa acaaaattgc
     1441 atcgagtgta ttgaatagta ttcccgattt catctttgtc attgatcaga accttcggat
     1501 cgtgatggtg aatgagcaca ttcgtacgat catgaaacaa gaggggatta ccggagatcc
     1561 tctcggcaag caatttaatg aatcttccaa gtttttatct ccggagtccg ttgccgggat
     1621 aacagatgta ttccgttcaa agaaatattc cctcgcgacc tatcatgtgg aagtgaacgg
     1681 gaataaatac atcacggaag cccgggtaat tcccatcatt aagaaccagg tggtggagca
     1741 gatcatgatc atcatccgtg atgtcaccaa agaaagggag ttcgaggagg tgaagctgcg
     1801 cgatgcagag cagaaagagt tgttgcttcg cgagatccat caccgggtga agaacaacct
     1861 cgccatcgtg atcagcatgc tcagcctgca atcgaggtac aatcccgatc ccgttctcac
     1921 ggggatcatc caggatatcg aactgagaat ccgttccatg gcattgatcc atgagcacct
     1981 gtaccgttcc gacaccctcg accgcatccc cttagccgac tacctgaaat ccttgtcagc
     2041 tgtcatcgcg agcaccttcc agcagccgca catcaacttc gagaccaagc tggaacccat
     2101 tgattcggat attgaggccg cgctgcccat cgggttgatc gtgaacgagc tgttgaccaa
     2161 cgcctttaaa tatgccttcc ctgacaagcg gcatgggaat atccttcttg agctgaagca
     2221 acttaacggg gagcaggtag aactcaggat cagtgatgac ggaatcggcc tgcccgaagg
     2281 attctcgatg gaagaaggat ccacgatggg cgtctttatg gtcagactgc tggttgaaca
     2341 actttatggc aagctggaaa taaccaatga gaagggagcg accttccggt taacctataa
     2401 ccataaaatc atctgatatg gaaacaaatg aaacctggat cctggggatc cgcgtcagtg
     2461 accgcgttca tgccatcacc gaggtgcaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn gaataagcca cacagatatc cagcggcatc tcggggatcc cgaacatctt
     2641 gtaacctgcc gagttagcca gcatgatatt tcccacctgc gggccattgc catgggtaat
     2701 gacaatatta taatcgcgtt ttagtaattt cacaaggcat tcactagtag catgggcatt
     2761 ggcttcctgc tcatcaatcg tgcctttctg cccactcatc agcagggcat ttcctccaag
     2821 tgcaacaact gccagtttct tcatagaaaa tgatttattg cgtaaaagta agaaaatccc
     2881 cggacttggg aggagatgat cgtccgcctg ttatcgggag ggtttttccg ggcaggattc
     2941 gtccccgttt ttggggagcg tgaagataaa ccggcttccg tttcccggtt cactctcggc
     3001 ccagatacga ccgccatgtt tctctacaaa ttccttgcag aggatcaggc ccaacccgtt
     3061 tcccctttcg ttggcagttc cgggagtagt gtatttttcc gagatcgacc agagcttctt
     3121 catattcttc tcttcaatcc cgacaccatg atcaatgacg gaaaccgaaa cttcatgatc
     3181 ttgggactct gtttgtatat ggatagcgct accggggata gagaacttca ccgcgttgga
     3241 gatcaggttc cggatcacgg ttttgatcat gttcttatcc gccgtgacga tgatgggttc
     3301 ggggtgggca tcgggttccg gattcaaatc gatggcaatt tgcttctcgt cagcataaac
     3361 actgatatta cagacgacat ccctgcacac ttccggcagg ttgagcggtt gtggattgaa
     3421 aagtacttta tcctgctgtg actttgccca ggccagtaag tcttccagca ggaaaaaggc
     3481 ctgtttcgat tcggtattga tgagggtaat gaatttattg atctcgatca gactcctttc
     3541 aggcaggtta tccagcagga tagacgtcag accgatgatg gcgttgaagg ggttccggag
     3601 gtcatgcgca atgatcgaca ataagaggtt gacgttctcg ctttttaccg tcatttcggc
     3661 agcgagtttt tgcaaccgga tctccgtctg tttaatcctg ctgatgtcgg taaacgaggc
     3721 gatgatcttt cgcatccccg ggaccggcgc caccgacatc atggcattaa cgatctcccc
     3781 ttttttgttg ataaaagaaa actcatattt atccggggcg ttttccggat cggtaaaccg
     3841 atgactatta tactccctga gacgctcctg gtcgcacggg acgatatgcc ttagccaggt
     3901 tgttccaatc tcttcttccc tggaatagcc ggtcatcttg cagtactctt cattcaccat
     3961 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatctcttc ttccctggaa
     4081 tagccggtca tcttgcagta ctcttcattc accatcgtga tgatggtgtc gggttcaaga
     4141 atggcaatgg ccgacgagtt cttatcaaag atggttttaa actccacctg gtttttagaa
     4201 accaactccg taaaagcatt gaactgttgt tctacctctc tcagcctttc attctctttc
     4261 ttcagtgctt gaagttcctt ttccaacgcc agtatccttt gggccggttc ctccacgaaa
     4321 atcctctcac ttcccatatc agtacaattt caaaaaacat aacaatcagg ttttcatctg
     4381 cttattcaat ttcatactga atttccaaag ttaaagttac ataaataatc acataaatta
     4441 aaaaatctgc ccggctgcac tcccgctttt cctttttatc tttgttgcat gcctaaaaaa
     4501 attgcgtttc atcattttgg ctgtaaggta aatttttctg aagcctcagc tttagcgcgt
     4561 cagttcaggg agagaggata cgaactgacc ggctaccggg agcaggccga catctacgtg
     4621 atcaatacct gcgccgtcac ccgggaagca gaaaaaaaat gtatttatgc catccgtcag
     4681 acacaccggt tgaatccgga agcgaagatc gtggtcatgg gttgtttttc ggagttgaat
     4741 ccggaagaga tccggagcat cgagggggtg accctggtgc tggggcaatc ggataagttc
     4801 cggctcttcg atctgctgga agacagtgaa ccggcgacgt cgccggctgc ctgcgatttt
     4861 gtcccctcct actcgatggg tgatcgtacc cggtcgttcc tgaagatcca ggacgggtgc
     4921 gattattatt gcgcctattg caccataccg ctggcccgcg gccacagccg cagcgacacc
     4981 atcgccaacg tggtgaggga agcccgtgat atcgctgccg ccggcgtccg cgagatcgtc
     5041 ctcaccgggg tgaacatcgg cgaattcggc cgtcatcaac aagagacctt tctcgacctg
     5101 ctgatccggc tggaacagga agtcgacatc ccccgtatcc gcttctcctc catcgagccg
     5161 aacctgctga gcgacgagat catccgtttg gtagcctctt cccggaagat catgcctcac
     5221 tttcatatcc ccctgcagtc gggctcggac aagacgctcc gggccatgca ccgcaagtac
     5281 gataccgcgc tctatgccgg acgcatcagc accatccggt cgttgttgcc ctatgcctgc
     5341 atcgccgcgg atgtcatcgt cgggttcccg ggcgagaccg acgccgactt cgacgatgcc
     5401 taccggttta tggaaggact tgacatctcc tacctccatg tgttcagcta ctccaggcgc
     5461 gatcataccc gggcagcagc catggaagat caggtgccgg ggcacatcat ccacctgcgc
     5521 agcgaagagc tccaccggct gtcggagacc aaaaagcacc gtttctacga acaaaacagg
     5581 ggacgccgtg aggaagccct gcttgaatcg acgaatgtgg acggcatgat gcatgggttt
     5641 accggtaatt atattaaagt gaaacacccc ttcgaccccg ggctgacaaa ccaaatcgtg
     5701 ccggtgatcc ttgacagacc ggatgacgac atgaccttta tcatctgaaa cagacaccat
     5761 gaacctggaa acaataacgc gtaaaacctg cgacatcgtc agcttggctg ccgccttcat
     5821 ccgggaagaa tcgacccggc tcagcgcatc ggatatccgg gcaaaatcgg cccacaacta
     5881 cgtcacgtac atcgacgaac aatcggagaa gctcctcatc ggggagctgt cgaagctggt
     5941 gcccggcagc gggttcattg ccgaggaaga tccttcgctg caacgcaaag aactcaactg
     6001 ggtggttgac ccgctcgacg ggaccaccaa tttcatccac ggactaccca tgtacagcat
     6061 cagcgtggcc ctgatggaag gtgacgaagt catcgccggc gtgatctatg aagccggaca
     6121 acaggaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ggaaggtgac
     6241 gaagtcatcg ccggcgtgat ctatgaagcc ggacaacagg aatatttcta tacctggaaa
     6301 ggcgctccat cgtacctgaa tcaaaaagcc atccgtgttt ctgtaacaaa gaaaatggat
     6361 gacagtctct tcgctaccgg atttccctat tatgattatt cccgtctgga taaatatatg
     6421 gaactgtttc gttttatgat gcaacacacc cgtggcatcc gccgattggg cactgccgcg
     6481 gccgacctgg cctggacagc ctgcggacgc ttcgacggat tctatgaata cggcctcagt
     6541 ccgtgggatg tcgccgccgg aacgatcatc gtagccaacg ccggagggac cgtatgcgat
     6601 ttcaggtcag gcaataactt tatcttcgga aaagagatag tggccaccaa cggcatcctg
     6661 catgaggagc ttatgaaaga gattcatcat attttctaac agcatcgata ccgatgagcc
     6721 tccggtccct gaaacgagtc cttttcccgg ttcttctgct gatgctgacg acagaagtcc
     6781 tctccggcca gaattcccgg agggaaatca acgccgtgaa agccgtttcg gacatccgga
     6841 tcgatggcgt gcttgacgaa cctgcctggg ctgacgctcc ggttgccagc ggtttcacgc
     6901 agtatgagcc ctataatggc aaaatgccgt cgttccggac agaagtaaag tttctctacg
     6961 acaacagcgg tttatatgtc gcagcgatga tgtacgatcc tcatcccgac agcatccccc
     7021 ggcaactcgg tccgcgcgat tccgaatggc tcaacgcgga tgacctcacc gtcatgatca
     7081 gcccgttcga tgacgggatc aacgcctccg tcttccagtt gtattcttcg gatgtacaga
     7141 ccgactataa gctgtctttc accaacgggg ccgacaacac cgaccgcacc tgggatgcgg
     7201 tgtggcaaag caaatcggtc atcaacgaca gcggctgggt cgccgagatc cggatcccct
     7261 ggtcggccat ccgcttttcg gaacaagggg agatgtcgtg gggaatcaac tgcttccgga
     7321 atatccgccg gtaccgcgaa aacagcagct ggaatccggt cgataataag atccagggaa
     7381 aggccaacca ggaagggctg ctgaaaggca tccgcgacat caaaccgccc ttacgtcttt
     7441 cgctgatgcc ctacctgtcg ggatacctcg agaagaaccc cgaagtcagc gactggcaga
     7501 caagcttcaa ctacggggcc gacctgaagt acggcatcag ccagagtttc accctcgaca
     7561 tgaccctgat ccccgacttc gggcaggtgc aatcagacta caaaatctac aacttcaccc
     7621 cgttcgagat ccagtacgat gaaaagcggc agtttttcac cgaaggcacc gagttattca
     7681 gcaagggagg cgtcttctac agccgccgta tcggnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnngtcttc agccaggcgc tgaaaaacaa ttcctacatc tcgctgctga
     7861 acaccaacgt ctataccccc gagacaggct attgcgcgga tgtggcgggc accgaattca
     7921 aatttgccaa caagaaatac acgtacgggg tttgggggac gttccttgtc agccagaaat
     7981 tctccgagtc caccctcccc gaccgcggct atcattacaa cctctcggtg gggaaactca
     8041 gcggtaactt ccagttcacc ctgtcacagc tgctggagac cgacaaatac gacccgaacg
     8101 atctggggta taaccagcga aacaacaagt tcgagaacgg cctggacctt tactacaaca
     8161 tctacgatcc cttctggaaa gtgctcaact ggtccaatta tctctacatc ggctacgatt
     8221 gcatgtacga agacctgaag tacagcagtt tctatatctc agccgagacc atgaccaaca
     8281 cccggaagta cctcacgctc ggctccaaca tggaaatcaa gcccgtcgat gctcacgatt
     8341 actatgaatc cagggtcccg gggagggtgt acatcgatcc ggcttcaaca gaagtcagct
     8401 tttggttctc caccgattac cgtaagaaat ttgcctttga tggctcggtg gccggtttct
     8461 attgcccgga attttccgcc acggggtatg ccgtttcgtt ggggccacgg ctgcagccca
     8521 acaagcgtct gacactgatc tgcgagctgt ccggggagtt cctgcaaaat ttcaacgggt
     8581 acgtgatgga ctcgctggat gcgatgcaac agccggtgat catcttcggc aaccgcgatg
     8641 tgaacaccat taccagcacg ctcaacggga cgttcatgtt cagcagcaaa atgagtctga
     8701 acctgcaagc ccgtcattac tgggtgacag ccaggtatca ttcgttctct gacctggatg
     8761 aaagcggaca tctgcaaccc aacgattatc agggagcgca tgatctcaac ttcaacctgt
     8821 tgaacatcga tctggggttc acctggaatt tcctgcccgg cagccagttg tcgttagtat
     8881 ggaaaaacgc catcaccacc cagggcgacc aagtggaaga tgacttctta cggaatttca
     8941 accagatgat cgaatcgccc gcctccaaca gcttctcggt aaaggtgctg gtttacctgg
     9001 atgccttgta tctgaagaag aaaaaataag cacggcgaag cagcggttaa aaaaatcgcc
     9061 tgaaagatgg aaaattttgt atttttgccg ggcagaacgc ggtagtagct cagttggtag
     9121 agcatcagct tcccaagctg agggtcgtgg gttcgaatcc catttaccgc tctgaagatt
     9181 cagcgattaa ccggatgagg aaagaataac gtttcaacat ccggtttttt tattgttcga
     9241 aagagccctg cccaccgggg ctaaacaatt cttcgggatt ttgcttggaa tgtgagaaaa
     9301 ggaatgattt tactatgtcc ggtcgatcaa ggagatggtg aagacagttg cacaaaagcg
     9361 ggagcgctga aggaggttac ccccggaata cggcagcgca tcttggacgc acacaacctg
     9421 gaaatcgaag ttgatcttca ataaaccaaa cagtgttcgg ttctgcgaaa gtctattcca
     9481 ctgtttttca ttctctttaa catctttcac cttgttaata aatatttttt gatggatgat
     9541 atgggtattt aaaactaatg tttaactttc atgcgtttta aattgaatta aagagaagaa
     9601 actcattcgt taaaagagaa tcaaagggaa atacttcggg ggcttacttg gtagttgcaa
     9661 agcgaagaaa agacaatgac accgaaattg gcgtagaata tggtttgaga ataaaaaata
     9721 taatgagaat caaagaaaaa aggttttttt ttgccgtccc tcagaaagaa ataattaatg
     9781 ccaacacaca tttggcacat tgccttttgc cccgacacac gaagccaact cttcggcaaa
     9841 atcaaaagat ccatttcttg ccagcgcttt aattgacatt gatatcttaa acatgatttt
     9901 cagaaatata tctttgtaaa ataataaata tttataaacg atgcttgaca ttattatttt
     9961 taatgtagaa ttaggacagt gtattttctt ttatccaaga cctgataaaa aagagttcgg
    10021 acttatggtt gattgtggaa atacacctaa ttttgagcca attgataaac tcattgaatg
    10081 gaatttactt ccgaaaaaat cagacgaaag aaatttaaag tatttactga aaagtttaac
    10141 actcactaat tacgaccaag atcatttttc aggtttacca tatttgcaga aaaaagtaga
    10201 aatagaaacc acaaggtttc cgaaaaacat ttcatcaact gatttacgaa aaataaaaga
    10261 cgaaatcaca gaaccaattg aagcagttat taaaattata gacaatgctg gaaaagggat
    10321 tacacttgat acaccttatg agaagagatg tttccattta gagcaagatg attttcctga
    10381 tgaagatatt gatactaaca agttaagcca aattgttttc gtaacattca aagatgttcg
    10441 tatttgtatt cccggtgatt tgacttctga tgcatgggaa aaaattttaa agaaagcaaa
    10501 tgtcatagac tacttgaagg gaacgaagat ttttatcgct tcacatcacg gtagagaaga
    10561 tggattcaat gaaaatgttt tcaaatattg caagcctgaa gtaataatac tttcagacaa
    10621 agatattatt cacaaaacac aagaaggaca gactcaaact tatgcaggca tggttcaagg
    10681 aaatggaatt attttaaatg gaaaaacagg tcaaccaaga aaagttttaa caacaagaag
    10741 cgataaacat atttggatac ggattgaaga caacggagca agaacttaca aaacaattac
    10801 tgaataattg aaaaataaaa agcatgaaag acatcattga taaattgtct acttacaaca
    10861 tattcaatta ttttctgcca ggaatattgt tcgtaataat tgcaaagtat ttcactgact
    10921 acgacatgat acttaaaaac aatctcatag gtacttttct ttactacttc ataggaatgg
    10981 taattagccg ttttggttca ttggtgattg agcctatctt gaaaaaaatt agatttgtta
    11041 aattcgctaa ttatagtgat tttatttccg cttcaaaaga agattccaag attgaattgt
    11101 tttctgaaat caacaatacc taccgtacaa taactgcaat gttttttctt ttactgttgc
    11161 tcaaaactta taattatttt gatacacaat accactttgc aaaaggaatt tcctttggaa
    11221 tattaatcat tttaatctta cttatgttcc tgttttccta tagaaagcaa acaagttata
    11281 ttaccaaaag aattacaaca gctaaaaaat caacgagtat atgaccgaac taatttggga
    11341 aggaaaatat aaagacggga agaaagttgc ccctgtgcga attgcactcc cctttcaaac
    11401 cattgaaacc ataaatgaaa gtgcgcaaca aaggcaaaag acccttgatt tctttgccaa
    11461 cgagcatact gcacaataca ataaggaatg gcgcaaccgc cttatctggg gtgacaaaaa
    11521 atatgtactg ccaagcttac tgcctgagtt tgctggaaag gtaaatctca tttacataga
    11581 cccacctttt aatgttggct ctgacttttc ttttacagct accattgccg acaatccgga
    11641 aacagaagaa gacgaagcca ctacttttgt taagcaacct agcatcattg agcagaaagc
    11701 ataccgcgac acatggggaa aagggcttga cagctatatg caatggtttt atgaaacagt
    11761 actcttgttg cgtgagttac ttactgcaga tggtagtatt tatgtccatc ttgactggca
    11821 tgtttcgcaa tactgtaaag tgattttaga tgaagtattt ggctacgaaa atttccgaaa
    11881 tgaaattgtt tggcaaagaa caacaaatac aggtagcagt aagggaatgg caaataagct
    11941 cagcactgat actgactcta tattttatta ttcaaaaaat actaaagcga cctttaacaa
    12001 gcaatataag ccgtattcag atgaatatct tgctaggttt aaatttaaag acgataaggg
    12061 aatgtatcgt tggcaataca tggctacata ctctgaaaaa aaattgaaag aatttgagtc
    12121 aaaaggaatg attcgctggc aaaaaggttc ttccaatcct gaatataaac aatatattga
    12181 tgatctaaag ggcattcctc taaataattt atggactgat atttttcacg ttaatccaat
    12241 ggctattgag aaagttaatt atgaaactca aaaaccagaa gaacttttag agcgaattat
    12301 taaaataagt agcaatgatg gagatttaat acttgattgt ttttgcggaa gcgggacaac
    12361 agcagccaca gcagaaaaaa ataatcgcag atggattacc tgcgatttag gtaggtttgc
    12421 cattcacacc gcacgaaaac ggtttttagg aattgacaat gtaaaaccct tcatcgttca
    12481 gaatcttgga aaatatgagc ggcagcaatg gataaaagca gagtttgaaa atgctgagaa
    12541 tcgattacaa caggaaaaaa cgtataaact gttcataaat gaactttacc atgcaaaatc
    12601 gcttaacggt tacacatgga ttcatggagc aaaggcaggc agaatgattc atgtaggcag
    12661 tgttgaaaca cctgtaaccg ttgaagacat caaagccacc attcttgaat tttggaaatt
    12721 ggtcggcaaa gaaaaatcaa ccactaccaa tggcattgac tttttgggtt gggactttgc
    12781 ttttgatgta aacgaaacag ccgcacaatt tgctacaagc aataaggtag atgtgaagtt
    12841 taaaaaaatt ccgagagaag ttttagaaaa gaaggcagtt gaacaaggcg acattaaatt
    12901 ctacgaattg gcttcactaa aagttaaaac gaaaattgat aagaaaaaag caagcattac
    12961 attagacaac tttattatac cacctgacga catacctgaa gatgtaagag aacacataac
    13021 acattggagc cagtggattg actattgggc tgtggattgg aattactgtg acgatacttt
    13081 tcataatgaa tggcagagtt accgcacaaa acaaaattca aagattgagt taacaacagg
    13141 aaaaatctac gatgaaaatg gaaaatacac cgtgcttgta aaagtgatag atattttagg
    13201 aaatgacaca acaaaagcaa ttcaaattga aatttaagaa tggcaggaac cttttcgcaa
    13261 atatatatcc aagtggtttt tgcggtgcaa aacagggaaa gcatgattca gtcgtcatgg
    13321 gaagaagagt tgtataaata cattgccggt attatcagga ataaaggaca aaaattgctg
    13381 gcaataaacg gaatgcctga ccatattcat tttctttttg gaatgaaagc atcgtgttgt
    13441 ttgtcagatt tggtgaggga aataaaaaaa tcatccaatg attttattaa agataagaag
    13501 ttttcaaagc ctaaattcaa ttggcaggaa ggatatggag cgttttcgta cagtcattcg
    13561 cagattgatt ctgtgataaa atacattatg aaacaaaagg aacatcataa gaagaacaca
    13621 ttcagggaag aatatttgga ttttttgaaa aggtttgaaa tcgaatttga agaagaatat
    13681 ttatataaat ggatagaata acgaatccga aggattcaaa tgattataga aaaaatgttc
    13741 ggttatttat acgaccccga tggggtcgta tattccctat acgattggat tctataaaca
    13801 tcaaatccct ttgggattaa aaaaataaaa tggtaaaaag aaaaaaaaac aatccttcac
    13861 aaaaagatat gttcaacata caagaacagt tgagcacagc tgcttgtgtg cccgctattc
    13921 gtgaagctgt caagggttgg agagtcgacg gctataaagg cattacagga acaacgagag
    13981 agttattaaa cttttggttc cgcacagacc atgttctgca aaatgggaac agttttcgtt
    14041 ttcacacagc gcagcgtgaa gccattgaaa ccttgattta tatttttgaa gttgccaaag
    14101 tcaaaaaccg aaaggaacta cttgagaagt ttgctttcac acaaaaggat ttacgtttac
    14161 cgccttatga tgaatttgcc cgttactgtt tgaagatggc aacaggtagc ggaaaaacat
    14221 ttgtaatgtg catgagtatt gtctggcagt ttgctaatta tctgagagga gaaaagggtt
    14281 atgccaacaa ttttttaatt cttgctccca atgtcattgt ttttgacaga ttgaaaacag
    14341 attttgaaag cggttatgtt tttagaacac ttccgttaat tccaaaacat ttgctttggc
    14401 tttgggatat ggattattac atgcgaggag atacggaacg cagccacagt cagggagcga
    14461 tttacctgac aaatatacag cagttttacg aaagaggaac gcgaaataat aatgaagaac
    14521 cggaagtgat gactacaatg cttggcagca taccaccagc acaaaaaaca gaaatcactg
    14581 acttcgatga acgcatttca aaacgtgatg gattgttgat ggtgataaat gatgaagcac
    14641 atcatacaca tgaagaagat agcgagtgga ataataccat tcgtaaactt catacacaaa
    14701 aaccaattgc tgcacaactt gattattcag caacacctcg ttacagcaaa ggaagtttat
    14761 ttgcatggac aatctttgat tatcccttaa aacaggcaat ccttgacaga attgtgaaac
    14821 gccctatcaa aggaatttca aaattagaag aatcgaaaag cacagttgcc agtgttcgct
    14881 acagcggatt tttaacagca ggtgtggaaa ggtggagaga atacagcgaa cagttaaagc
    14941 cgttgaaaaa gaaaccgata ttgtttatta tgatgaacaa taccgaagaa gccgacgaca
    15001 ttggtgattg gatacgaaca aaatacccaa aggaatttgg tggtgaaaaa acattgatta
    15061 ttcacacaga caaaaccggt gaagtttcaa aaaaagattt ggatgcagca cgtaaaactg
    15121 ccagagatgt tgaccacgat gaaagtcctg taaatgtcat tgtgagtgtg cttatgttac
    15181 gtgagggttg ggatgtgcaa aatgttacag ttgttgtcgg cttgcgccct tattctgcaa
    15241 aagcaaatat tcttcctgaa cagacaatcg ggcgtggttt gcgattgatg ttccgaaaca
    15301 tgacagatgg ctatacagaa agggtagaca ttatcggcaa caatgcgttt atggaatttg
    15361 ttgaggactt ggagcagtta gaagatttga aatttgaaac atttcagatt ggaaaagaca
    15421 aactaaaaat tctcacagtt cagcccgaag aaaaaaagaa agagcttgac atcagtattc
    15481 ctaatatttc accactacta tcacgtaaaa aatctttagc ggaggaaatt acagctattg
    15541 atataatgaa atttaatatt aataagcttc cattaaaagg aaaggaaatc acagacacaa
    15601 agaaatttat ttatgaggga agggatattc tcactgacga aaaactgtta gagagagaat
    15661 acatgattcc acaggcacaa acggcagaag aagtaatcgg ctattatgca agacgaataa
    15721 cccagaatat taaacttcca tcacaatttg ccgttttagc tccaaaggtt cgtgccttct
    15781 ttgaacgcaa agcattcgga cagacagttg atttgaacga ccctgttgta atcaaagcaa
    15841 taagtagcaa cattgcaagt tatgtggtaa taaaggaatt tgaaaaagta ttgcgtgaat
    15901 tggtagttga acagaaagag ccacaactgt tatctgaaaa tcgttttctt tcttccactc
    15961 cgccgttccc gacttcaaag aaagttttgg aagcgaaaaa aacggtgttc aactatacgc
    16021 cctgcgacaa tgagtatgaa tatgaatttg caaaattcct tgataaatcg gaagacatca
    16081 tttcgtttgc taaactgcct gagcaatttg gtttctgtat tcaatatact gatactttag
    16141 caaacatcag aaactatttc cctgacttta ttgctttgca tgaagatggc agccgttgga
    16201 taattgaaac caaaggcaga gaagacgttg aggtaaagtt aaaggacaat gcagccataa
    16261 actggtgcaa cactgcaact gaactcacaa aagaaaactg gaattacctg aaagttttgc
    16321 aaaaagaatt tgaagtacta caccctgaca actttgaaga attgttggcg ggagtaaatc
    16381 caccgacttt gtttgattaa gaaagacaat aatggcaaaa gttgaaatac ccccatacaa
    16441 tgaattgcaa tcatacgctg atgttgttaa gcgtgtcaaa aacaggcaga tacatttgct
    16501 tttaggaaat ggtttcagca tgtcttatga taacaacatt ttttcttaca acgcactgag
    16561 caaatttatt cacgtaatta agaacaagga gctacaacaa ctctttgaca tcgttaacac
    16621 aaacaacttt gaattattga tgcagcaact tgataatgca tcacaaatcg cagaagtatt
    16681 tggggcagac aaaaaagttg taaagaaaat aagagatgca agtgaaacat taaaaactag
    16741 cttgattgaa gccataaagg aattacaccc tgaccatgtt tttacaatcc ctgaaaataa
    16801 aagtcaggca tgtgcaaatt tcttgaatac atttctttca caaggcggtg gagtttttac
    16861 aaccaattac gatttgctac tatactgggt ttgtgagagg aatcaacttg aaaacagagt
    16921 agatggtttt ggcagagact cagaagaaac agacgaatgg gttgagcctg atgaaagaca
    16981 atattctgaa ttgcgttggg gaaaatacaa agaggaacaa tccatttatt acttgcatgg
    17041 agctttgcaa atctttgaca caggaatcga tatcataaaa gaagaataca caagcgaaca
    17101 ttatttactt gagaacatca aaagaagaat ggcgaaaaaa gaatatccaa tattcgttac
    17161 agcaggaacc ggtattgata aactaactca tattacgcac aacaaatatc tttcgttctg
    17221 ttatgaaaac cttactgcta ttgaaggttc gctaattgtt tatggattcg gatttggaga
    17281 atatgatgac cacattatta aagcaatcaa caaagctgga aaaaaacgat tagacgagaa
    17341 cgaaaagtgg cacatgctga aaagtgttta cattggagtt tattctgaaa acgattatga
    17401 atatatcaaa aaaattcaaa aacagtttaa agttccaata agattgtttg attcaaagac
    17461 agtaaacatt tggggataaa tatgaaaaac caacgcagca ccttattcgt ccaaaaagag
    17521 atataagcga aatacttctt atacctactc gttgccagca aggttaaaaa agaaaaccgt
    17581 attttaattg tcatgaccaa taaatgcgct atatttctca tgaaaattaa gatataaaga
    17641 catggataag aacgaattca tcagaaattt ccgcattgca tttggaaact atgaattacc
    17701 gattgcaatt tggtattcag aaaaagctgt tgcccaagaa gagaaaacaa gaggttgttt
    17761 tattaaatat ttaaagccaa ccagagaagg caagattgtt agtttaagct tagaaacaat
    17821 atcatgtccg ggagcaaaac tttattgcgg gtttacagga gctgctcctt tcattccaaa
    17881 tttcgtttca gaaaaagagc gctataaaaa aacgcctgaa atggtaactg atttcattgc
    17941 tgacttaaat atgccggata agtcaaaaca gtttctcaat tttgcatcaa ttgatagaat
    18001 tgaaaacttt gataatattg atgcactgat tttttttgcc acacctgatg tattggcagg
    18061 cctcgtttcc tgggcgcttt acgataccaa tgaacccgat gcggtttcag ttccttttgg
    18121 gtctgggtgt agttcaatgg tttctcaag
//
LOCUS       DAOPYB010000045        18081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3246_length_18081_cov_9.134916, whole genome shotgun sequence.
ACCESSION   DAOPYB010000045 DAOPYB010000000
VERSION     DAOPYB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18081
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3246_length_18081_cov_9.134916"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKH58_05245"
     CDS             complement(<1..559)
                     /locus_tag="PKH58_05245"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HNX88464.1"
                     /translation="MKKLIIACSATLMLISCSSSKHLNVIVYNQADFDRNKEIIEINK
                     SSVVNKLGLKTNERFILLDHDSNQVDFQIASNSNQSTDSLLIFPVSVKANSKASYTIK
                     KGIPAPFQSKAYGRFVPERKDDYCWENDRIAFRVYGPTLQATGEISSGIDVWTKRTDK
                     LIINKWYADDLTGKQSYHTDHGEGLD"
     gene            complement(571..1365)
                     /locus_tag="PKH58_05250"
     CDS             complement(571..1365)
                     /locus_tag="PKH58_05250"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HNX88465.1"
                     /translation="MNLFDLTGKNALITGATYGIGFSIATALAEAGATILFNDVNQDL
                     VNKGIAAYKDLGITAHGYICDVTDENAVNQMIAQIENEIGVVDILVNNAGIIKRVPMH
                     EMSAADFRKVIDIDLNGPFIVSKAVIPGMIKKGRGKIINICSMMSELGRETVSAYAAA
                     KGGLKMLTKNICSEYGEYNIQCNGIGPGYIETPQTAPLREKQPDGSRHPFDEFIISKT
                     PAARWGKTQDLKGPAIFLASEASDFVNGHILYVDGGILAYIGKQPQ"
     gene            complement(1453..4509)
                     /locus_tag="PKH58_05255"
     CDS             complement(1453..4509)
                     /locus_tag="PKH58_05255"
                     /inference="COORDINATES: protein motif:HMM:NF020658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase family polysaccharide lyase"
                     /protein_id="HNX88466.1"
                     /translation="MIKNYLLLLLILILLVNSTFAEKTNKEYDNPAILSFETIALNIS
                     RDGKSSLSISRDHFKHLKKSLKWSWNAPNAQWEIKQNVGYIPLKKKSDAFVSTFVFWV
                     YSEKPLKNEKLKVEFLKENQVKCYFDYNLDFSGWRGAWIAFNRDMQGTPVEGMDEVRF
                     TAPDTPEGTLYFDHMMLSSMQDVRQHTADFQAPFINKNTENHWLILLRSWNKDFDIPL
                     NSSFSISEKEGTEIISNRLRNLLLEGKKIKSVEDLRKSFDQYQISLNQDGSVKGLPVF
                     FERYGETYEYLGAERYNKIFDNPMGLSSAVKTLFDLAVSYNLSTDEKEKKQFSEMFIL
                     LTKHLLDQGFQAGSGMGTLHHLGYSMRDYYPAMFLMAKPLKENKLDKTIQQSMEWFAG
                     TGEVKTDTKDVEMDIDAFNTSLIGRLTSILMIDDVASKIRYLHAFSRWVDQGFKYSNG
                     TRGSFKADGSIFHHRHNYPAYAVGGLTGAVQSVYLLNKTNFKISQESHEILKKALLSM
                     RIYCNLRTWPLSLSGRHPDGKGQLNPEHYALLALAGSPDTKQVVDSELAAAYLRLIPK
                     ETIRSKDLKKAGFIAENSPNGNWSFPYSCLNIHRRDNWMVSAMGHSRYLWSTEIYRGE
                     NMYGRYINHGSIQILATGTPVSNEGSGFRQEGWDWNHFPGTTATVLPLKELRAKIKNL
                     DQFSGFEEMLLSDEAFAGGISLDNKQGAFAMKLHEHDKYNGSLRARKSVFFFDNRAVA
                     LGSDIQSALPLPTQTTLFQTYIDQINKSYYLNGKNITEFPYKETVKKEKTIISDGQNN
                     YFFVKNGEVQFSKSVQQSFDEETDAPTSNDFALASINHGGDLKNGRYEYLILIQPSEK
                     ELKLYLKAKEPSYIVHQSDSTAHIVFDKQTKMSGYVFFEPSTTANLLVKKVDTPCLIM
                     TDESIANKLTIAVSDPDLRFYDGKPDDNYDNNGKRLERSVYSRSWKDNPSKTSEINVT
                     LNGIWKTEKNYDFIKTETLNDTTILIVNCDNNRQDNSIKLKKTDSN"
     gene            complement(4610..5806)
                     /locus_tag="PKH58_05260"
     CDS             complement(4610..5806)
                     /locus_tag="PKH58_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNX88467.1"
                     /translation="MKHILIASILTFTIVGCNSATSKTNQRNQFSDIISTAEQQIGFQ
                     VKLIEDSARFINPRTVVDGVVKYIPSDDWTSGFFPGTMFYMYDLTKNKKWLDLGVKYT
                     ETLDSIKYLKWHHDVGFMINCSYGNALTYTENEKYRDVIIEAAKSLATRFRPIPGVIQ
                     SWNEDKGWQAKRGWMCPVIIDNMMNLELLFKATQFSGDSTYYKMAVSHADNTLANHFR
                     KDWSCYHVIDYDKIKGGVRHKQTAQGYSDESAWARGQAWALYGFTMCYRYTHDPRYLE
                     ATENIYKFIFNNKNMPKDLVPYWDFDAPNIPNEPRDASAAAIIASALYELVGYGKTEY
                     KETADKIMSSLSSPVFTALVGTNGNFLLMHSVGSIPHGHEIDVPLNYADYYYLEAVIR
                     QRNIEK"
     gene            complement(5828..9019)
                     /locus_tag="PKH58_05265"
     CDS             complement(5828..9019)
                     /locus_tag="PKH58_05265"
                     /inference="COORDINATES: protein motif:HMM:NF020658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase family polysaccharide lyase"
                     /protein_id="HNX88468.1"
                     /translation="MKRNVIFILFITLTTFVSGQNYSFEEPETPTDWIASGGDVLSVT
                     TEHFKDGNQSLCWITNGNNSLRVNFVSFLTNTSNGTRLQLYSPEVTNDTLIIEYYSAL
                     NLLRRKAFYLINYKGWRDFSRKYTEFSTSNSSQDIVSVKFTLKRTDTKVRKIYFDKVD
                     FNTTVAVDRVVGNQWVLDKNYFSGNTEPVTINNFDKDIPVQAASSQEIADLNLLRNKL
                     VRVPASPTLTVLVQARSYVDGLNIQRNTDGSVKGKVINNFPEYLTIDSITNIAVRLEV
                     LASDYLKNPTNSTITTRFSNYLDHILDQGIAEGCSFAIASSNYTPSRDIPARLLNAIN
                     ACTPEQKVELLKLVRWFTFYGNMYYPESIYKTSLVSDIIYLYLPHIMASAIFYPDDNT
                     AVRELKAFRRFIERNTEYIPGGNDILKPDGTGFHHNTHYNNYMYCYQTWVEYMNYLKG
                     TQYRISPEAYNRIKKAVVSVYRMATNDVNENHFFANSLAGRNPFVSGQKLYFNKTLFQ
                     NLISIGGDCMGTAMDEELAGKYNYFYKTNFYNVPTSNQEGFYQFNYSPIGIYRKNNWV
                     VTMRSPTTKFWGAEIYSNANRFGRYQSHGTLEVLYDGSSLAYSGEPTNNTGGGWDWNM
                     PQGATVVQYNTWQDLMPNRNLTDRFDQYTKTKDFAGALSWGDCGMFAADFDQIDSWGS
                     QRFTPTNLTFKKSMFAFDGMVISLGSNISSSGTYNSAWFTSTNLFQNIVTGVNGNFVV
                     NGNNVTKPYNTTLSSTTDNWFVTPTGTGYFIPKGNDDIVVKYDAQSTPKEDGSDYASP
                     VTTLNAAKAYLNHGVKPTSKNYSFVVVPGTNTTAMQAMTAQLQNNGGSIYKINSQTST
                     LHSIIYLPAQINAYAFFDAVSNISFGKIKSCSSSLLLLQKENIVDNKLSFAVCNPNLK
                     PVTDPIFGWVSSPSSITITLAGNWREETAIEGVTFYPPVNDETVVSFNLKDGEPVYFS
                     AVNYYTKVEDNKVNNWFIYQKNNDNLIIRFNELDSSDRRISVYSIDGKLLKSQISDGN
                     SQQVIINTGDIAKGVNICTVSNIEVSKTFKWIN"
     gene            complement(9048..10010)
                     /locus_tag="PKH58_05270"
     CDS             complement(9048..10010)
                     /locus_tag="PKH58_05270"
                     /inference="COORDINATES: protein motif:HMM:NF027724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5017 domain-containing protein"
                     /protein_id="HNX88469.1"
                     /translation="MKNKILTILIGCFVLWSCEDALKQPVKFNVTASTDEGTISGDAF
                     VVDSGKTITFNFTGDPDFITFYSGESGHEYVKRNLVETPAEEITSTLKFNAKPQYGII
                     EGTLKVYLSTTFEGLRGYDKLTDSLAVIKNQWTDISASCNLPTLSNGTVDVALPLNDY
                     MGKKLTVAFQYITNQNTSTQPTWIITGLQIENTMKSSGAVSAIKASNMGFQALDMLAL
                     TAPYKNTGGSGIWNLSNISTEIRIQSSPSGSSINDDWVISNPLIINSRLADTGVAIKK
                     MSMDLDAYNYTFNKKGKYIVTFLAKNANFESSYELIRQFEITVN"
     gene            complement(10074..11786)
                     /locus_tag="PKH58_05275"
     CDS             complement(10074..11786)
                     /locus_tag="PKH58_05275"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX88470.1"
                     /translation="MKTYIKIFIASLLTLTISSCEFLDKQPHELTPETYFNSESELQS
                     FLAGVYSPLGQEYFYGNYYPVYNAGGDDLSFYQRANSPVSIMTADANSTNQYISAYWR
                     LLYDGISRANILIENVDKNETISEAIRNRVKSEAVFLRAFYYFNLIQGWGDVPFRLTS
                     AQSVDNLSLPRTDKQIIYDSITNQIVKSIPYLNSAANTNPGYVTQSAAKGILARIYMF
                     RAGEHFRDNKTADNHVQKYFQEAKKWALEVKESGIHELTKSYSQVFTDLCQDKYNSTG
                     KKESIWEAEEAGNRINSPDWSAGRIGNVIGFGSTNDYSTVSPYQNQGGMSNPGYSYKF
                     VYASLKLFNMYESENDTARCDWNITPYEYVYASTAPKQVTGRNYYFGKRRAGLATVEG
                     LPCTDMTQSASNNNKTRCCAKYRRELETVLIKNKNYTPINFPILRYSDVLLMIAEAEN
                     EINSAPTQLAYDCINEVRRRAHIVEYQVGSLEQTTFRDAVKKERAMELCFEGIRRWDL
                     IRWGDFYKNMQSMRSSVNDPGWGSGFKYAASYYNVSENYNYYPIPSIELSVNKEITSN
                     NPGW"
     gene            complement(11805..14978)
                     /locus_tag="PKH58_05280"
     CDS             complement(11805..14978)
                     /locus_tag="PKH58_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX88471.1"
                     /translation="MKKLRIHLLLLFLIFSVGIFAQINITGTVVDETGATLIGVTVRL
                     KGTTQGTVTDINGKFGINVSKPTDVLIFSSIGYETIEKTVERNKPMLVTLTDNSKLLN
                     EVVVVGYQDMKRKDITGSVAKANVEDMLKAPVPTFDQALAGRVAGVNVSSSEGSPGNN
                     MNIVIRGKNSVTQDNSPLYVIDGFPVEDPKAGASINPNDIESIDVLKDASATAIYGAR
                     GANGVIIITTKKGKTGQLNINYDGSYGLQRITRTIPLMNAYEFVRLQSEVWNPAEMSS
                     TGYYKTIDNKTYTLADYANVEQYNWQDLIFHDAMQQNHSLSLTGGAADNRYNASFSYY
                     DQDGIVIESNYKRLQGKLGTDIRRNKLRINLSVNYSNSTQTGNSPSQTASSGMNNLFY
                     SVWGYRPVTQPGVPLNSLINNGLDSNVDTSIDYRFNPYLSLNNEYRKNNIAYTQFNGF
                     AEYEFIKGLKLKVSGGYTTDVRKLEVFNNSNTRYGSQLSIDKVNASVGASQSYTWLNE
                     NILTYQKLINKKHSINMLGGFTLQENDFKFYTMKTTNIPNESLGMAGMYQGTPNTNDS
                     GESEWSLMSFLGRVNYNYLSKYYFTASFRADGSSKLSPQNRFRYFPSTAVAWTFTEEK
                     FMRDFKKALTSGKLRLGWGVTGNNRVEEYARFARLSQESAGNGSYNDPSGYTHGVYSF
                     NNSIIKGTIPVSLANNNLKWETTSQWNAGLDLVFLNERISTTIDYYNKLTYDLLIKAT
                     LPISSGFGSAMKNIGKVRNEGLEFTLNTTNIKNKRFSWNSSFNISFNKNILLGLNEGQ
                     RSYITAEIVDRNFGGDNYIAKVGYPIGMMYGYLYDGTYKLDEFNVVGGNYSLKPGIAR
                     YTAENNTQPGYPKYKDMNDDGIINSDDQTFIGRGDPIHVGGFTNNFSYHNFDLSIFFQ
                     WSYGADIINANRLMFETSFAKRKDLNQFASYADRWTFDNPDSDIPRVNNSSSNLLYSS
                     RIIEDGSYLRLKTLSLGYNFPKKLIKELKINSLRVYLSAQNLFTWDNYSGYDPEVSIR
                     EKALTPNLDFSAYPRAASANFGVNLTL"
     gene            complement(15090..17015)
                     /locus_tag="PKH58_05285"
     CDS             complement(15090..17015)
                     /locus_tag="PKH58_05285"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNX88472.1"
                     /translation="MKKLLLSLVVLLSMTAMAEQKNGIVYVKPGGAGDGTSWANAMGD
                     IQTAINTARADKAARKDVWVAAGDYTIGTAIVMSDSISLYGSFAGTETAVDQRTKLTN
                     GKPWEFTNSTILTANNCRLIETASNFDMATIVDGFILTNGNGIGTTLSKSGGAAVVRN
                     NMVIQNCVIQNSTALDGAGGGLNMTGGTVKNSWIYNNRQTTNANGGGGIYINTASGNE
                     TTVENCKIEKNSSTIRGGGINVQGVGFTYLKNLYIVNNKSEDSGTPKAGAAIYANSAT
                     NTATNCIISNNSGLTTFYIKGNVFNCTMVNNIGGAYLAEASATIELSNNIFWGMYTDI
                     TETTATSLSGAANANATINNNATYNPIPTDKNWKTSGNIQFSSNISNGDVTDPAPGTV
                     GSGPKFYHVTRFRGTAAADEEILSLDSADWRIKYNSPCLNIGKTLVATNLDYLGVQRP
                     QGHPTASAQYDMGAYELPYRKMLIGEGTNTNGTVYDSNATQLPDGTILGYPEGTVSDF
                     LFEAKPGYKLLKAAYITGSDDLTSFDGQEVDITDKIDAAGIWHSHPLVKSLKLYAVWS
                     STSGLENIDYVYSVFATKNKIIFSKINQNDKVSVYTITGNLIFNKKADTPQFIVPVEK
                     GIYIVRINNLAKKVVVE"
     gene            complement(17105..>18081)
                     /locus_tag="PKH58_05290"
     CDS             complement(17105..>18081)
                     /locus_tag="PKH58_05290"
                     /inference="COORDINATES: protein motif:HMM:NF039745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNX88473.1"
                     /translation="GSAIYTQVDTDKANKIINCVFYGNKTKSALYLRGAAVYNSTIVN
                     NEGGGVYTATKDAQIYNSVFWAKNKSKATVSGVDVAGVEYKNNASITIPTFPNWTVAD
                     NIQLDTLISETNYPYFADPLNNDWTITYKSPLLNAASNSIAGVPTTDFLGLARPQGSA
                     YDIGAYELPYCNITVTFNTGGTVNSYTSGDIIAQPKGIAMNYTITPNSGYKISAVSYN
                     GTDVTSQLSENVYTAPALTGISTLNVTFETDPGTHLSNLKSTFKCFTSSNQITIEGIN
                     IGEKVIVFNALGAKVKEVNANASNISLHVSKGIYIVRAADQATKVIVK"
BASE COUNT         5112 a         3519 c         3296 g         6154 t
ORIGIN      
        1 tatccaatcc ttctccatgg tcggtatgat agctttgctt gccggttaaa tcatcggcat
       61 accatttatt tatgatcaat ttgtctgttc gttttgtcca gacgtctatt ccactgctta
      121 tttcgccggt agcctgcaga gtaggaccat atacccgaaa agctattctg tcattttccc
      181 agcaataatc atctttacgc tcaggcacaa atcttccgta agctttagat tgaaatggag
      241 caggaattcc tttttttatt gtatatgaag ctttgctatt agcctttaca gataccggaa
      301 aaatcaacag tgaatctgtt gactgattgc tattgctcgc aatctgaaaa tctacctgat
      361 tggaatcatg atcaagtaaa atgaaacgtt cgttagtctt caatcccagt ttgttgacta
      421 ctgatgattt gtttatctcg atgatttctt tatttctgtc aaaatcagct tgattataca
      481 ctatcacatt aagatgcttt gatgacgagc aacttataag catcaaggtt gctgagcatg
      541 caatgatgag ttttttcatt ttgatgatga ttattgcggt tgtttgccaa tataagctaa
      601 aattcctccg tctacatata aaatatgtcc gtttacaaaa tcagaggctt cagaagctag
      661 gaatatggcc ggtcctttca aatcctgggt ttttccccag cgggcagcag gtgtttttga
      721 tattataaat tcgtcaaaag gatggcgtga accgtcaggt tgtttttctc ttaatggtgc
      781 cgtttgtggt gtttcaatat aacccgggcc tataccgtta cactgtatat tatattcacc
      841 gtattcagag caaatatttt ttgtcaacat tttcaatcct ccttttgctg ctgcatacgc
      901 cgagacagtt tcacgtccga gttcgctcat catagagcag atattgatga tcttgccacg
      961 accttttttt atcataccag gaatgaccgc ttttgaaact ataaatggac cattcaggtc
     1021 aatatcaatc acttttcgga aatcagcagc actcatttca tgcattggaa cgcgtttgat
     1081 gatgcctgca ttgttaacaa ggatgtcaac aacacctatt tcattttcaa tttgtgcgat
     1141 catttggttt actgcatttt catcggtaac atcgcaaata tatccgtgtg cagtgattcc
     1201 taaatctttg tatgcagcta tacctttgtt tactaagtcc tggtttacat cattaaacag
     1261 aatagttgct cctgcttcag ccaatgctgt agctatagaa aaaccaattc cataagtagc
     1321 tcctgtaatt aaagcatttt tccctgtcaa atcgaataaa ttcatagttt aataagttta
     1381 taagttgtag aatatatttg taagtttgag aagataaaaa cgatttatcc aaaaagatgc
     1441 cagtaattga tgctaattgc tgtccgtttt tttcagtttt atcgaattgt cttgccggtt
     1501 gttgtcgcaa tttacaatta aaatcgttgt atcattcagt gtttctgttt tgataaaatc
     1561 atagtttttt tctgttttcc aaataccgtt caaagttaca ttaatttccg aagttttcga
     1621 aggattatct ttccaactac gtgaataaac actcctttca agtcgtttcc cgttattatc
     1681 gtaattgtcg tccggttttc catcgtagaa acgcaaatca gggtcagaaa cagcaattgt
     1741 taacttattt gctatcgatt cgtcagtcat tatcaaacaa ggtgtatcga ccttttttac
     1801 taataaatta gctgtggttg aaggttcaaa aaaaacataa ccggacattt tcgtttgttt
     1861 atcaaaaaca atatgagccg tcgagtcgct ttgatggaca atatatgaag gttcttttgc
     1921 ttttaaatac aatttcaact ctttttcaga tggttgaatc aatatcaaat attcatatcg
     1981 tccgtttttc aggtcaccac cgtgattaat tgatgctaaa gcaaaatcat tgctggttgg
     2041 agcatcagtt tcctcatcaa atgactgctg aacagatttg gaaaattgta cctctccatt
     2101 ttttacaaag aaataattgt tttgtccatc tgagataatt gttttctctt tttttacagt
     2161 ttccttataa ggaaattccg taatattttt tccgttcaaa tagtacgatt tgttaatctg
     2221 gtctatgtaa gtctgaaaaa gggtagtttg agttggcagt ggcagagccg attgaatatc
     2281 agaacctaaa gcaacagctc tgttgtcaaa aaagaataca gattttcggg cacgtagaga
     2341 accattgtac ttatcgtgtt cgtgcaattt catcgcaaaa gctccctgtt tgttatccag
     2401 cgaaatacca ccggcgaatg cttcgtcgga taacagcatt tcctcaaaac cggagaactg
     2461 atccaggttt tttatttttg ctcgcaattc tttcaaaggt aaaaccgtgg cagttgttcc
     2521 gggaaagtga ttccaatccc atccttcctg ccgaaaaccc gaaccttcat ttgagaccgg
     2581 agttccggtt gctaaaattt gtatagaacc gtgatttatg tatcgtccgt acatgttttc
     2641 gccacgatat atctcagttg accacaaata acgactgtgt cccattgctg aaaccatcca
     2701 gttatctctt cgatgtatat tcagacagga atatggaaaa gaccagttcc cgtttggaga
     2761 attttcagca ataaatccag cttttttcaa gtcttttgat ctgatcgttt cctttggaat
     2821 tagtctgagg taagctgctg caagttccga gtcaaccacc tgttttgtat ctggacttcc
     2881 cgctaaagcc agcaacgcat aatgttcggg atttaactgt ccttttccat cgggatgacg
     2941 acctgacagt gaaaggggcc aggtacgcag attacaataa atccgcatag ataagagagc
     3001 cttttttaat atttcgtggc tttcctgtga aatcttgaaa tttgttttgt taagcagata
     3061 aaccgattga acagctccgg ttaatccgcc tactgcataa gcaggataat tgtgacgatg
     3121 atgaaaaatc gagccatcgg ctttgaagga tcctcttgtc ccgtttgagt atttaaaacc
     3181 ctgatcgacc caccgggaaa aagcatgaag ataacgtatt ttcgatgcaa catcgtcaat
     3241 catcaaaatg ctggtaagcc ttccaataag actcgtatta aatgcatcaa tatccatttc
     3301 cacatctttg gtgtcagttt ttacttcgcc ggtacctgca aaccactcca ttgactgttg
     3361 tatggtttta tccagtttgt tttcctttaa cggttttgcc attaaaaaca ttgcaggata
     3421 ataatccctc atgctatatc ctaagtggtg taatgtgccc attccgcttc cggcctgaaa
     3481 tccctgatcg agcaaatgtt tcgtcaacag aataaacatt tctgaaaatt gttttttctc
     3541 tttttcatca gtcgaaagat tgtaactgac agctaaatca aataaggttt ttactgctga
     3601 acttaatccc atcggattat caaaaatctt gttgtaacgt tcagcaccga gatattcgta
     3661 ggtttcaccg tagcgttcaa aaaaaacggg aagccctttt acagatccat cctggtttag
     3721 tgaaatttga tattgatcga acgattttct taaatcttca accgatttta ttttctttcc
     3781 ttctaatagt aaatttctta agcggtttga aataatttca gtaccttctt tttcggatat
     3841 tgaaaaagaa gaattcagag gaatatcaaa atctttattc cacgaacgaa gtaatataag
     3901 ccagtgattt tctgtgtttt tattgatgaa aggtgcctga aagtcagctg tatgctgacg
     3961 cacatcctgc attgaagaaa gcatcatatg atcaaaataa agtgtgcctt caggagtatc
     4021 gggcgcagta aaacgaactt catccattcc ttcaacaggg gttccctgca tatcacggtt
     4081 gaaagcaatc caggctcccc gccagcctga aaaatctaaa ttataatcaa aataacattt
     4141 gacctgattt tctttcaaaa attccacttt cagtttttcg tttttcaatg gtttctcaga
     4201 ataaacccaa aaaacaaaag tagaaacaaa agcgtcgctt ttctttttca gcggaatgta
     4261 accgacattt tgtttaatct cccattgagc atttggtgca ttccacgacc atttaagact
     4321 ttttttcaga tgtttaaaat gatctctgct gattgataaa gaggatttac cgtccctcga
     4381 aatattgaga gctattgttt caaaagaaag aatagcagga ttatcatatt ctttgtttgt
     4441 tttctcagca aatgtcgaat ttactagaag aatgagaata agtaagagca gtaagtaatt
     4501 ctttatcatg ttgttaatgc atttgttttt aatgagaact ttgaatttca gacaattatt
     4561 gtctgaaatt catattcaga agtaatttgc tattttctct gtattaactt tatttctcaa
     4621 tgtttctttg tctgattact gcctcaagat aataatagtc tgcatagtta agcggaacat
     4681 ctatttcgtg tccgtgagga atacttccaa ccgaatgcat caagagaaaa ttgccgttcg
     4741 ttccaactaa tgctgtgaat acgggactgc ttaacgatga cataattttg tcggcagttt
     4801 ctttgtattc ggttttacca taaccaacaa gctcatacaa agctgaagct attatggcag
     4861 ccgcagaagc atctcgtggc tcgttcggaa tgtttggtgc atcgaaatcc cagtatggaa
     4921 ccaaatcctt tggcatattt ttattgttaa aaataaactt gtatatgttt tctgtagctt
     4981 ccagatacct gggatcgtgt gtatatctat aacacattgt gaatccatac agtgcccatg
     5041 cctgaccacg tgcccacgcc gattcgtcag aataaccctg agcagtctgt ttgtgacgaa
     5101 ctccaccttt tatcttgtcg taatcaatta cgtgataaca gctccagtct ttacggaaat
     5161 gatttgccag cgtattgtcg gcatgtgaaa cagccatttt ataatatgta gaatcacccg
     5221 aaaattgagt agccttgaac agtaattcca aattcatcat attgtcaatg attaccggac
     5281 acatccatcc acgtttagcc tgccagcctt tatcctcatt ccaggactgt attactcccg
     5341 gtattggacg gaaacgtgta gccaaagatt tggcagcctc aataatgaca tccctgtatt
     5401 tctcgttttc ggtataagta agtgcattac cataactaca gttaatcatg aatccgacat
     5461 cgtgatgcca tttcagatac ttgatcgaat ctaaagtttc ggtatatttc actccaagat
     5521 cgagccattt tttgttttta gtcaggtcgt acatgtaaaa catggtacca gggaaaaaac
     5581 cactggtcca gtcatcagaa ggtatatatt ttactactcc gtcaactaca gttcttggat
     5641 taataaatct ggcagagtcc tctatgagtt taacctgaaa tccaatctgc tgttcggcag
     5701 ttgaaatgat gtctgaaaac tgattacgtt gattagtttt actagttgca gagttgcagc
     5761 caactattgt aaacgtaagt attgatgcta ttaaaatgtg ttttattttc atcttgttct
     5821 tataatttta attgatccat ttaaaggttt ttgatacttc aatgtttgaa acagtacaga
     5881 tattaactcc tttagcaata tcacctgtgt taatgattac ttgttgcgaa tttccatctg
     5941 atatttgcga ttttaataat ttaccatcga tcgaatatac agatattctt ctgtcagaac
     6001 tatctaattc attgaatctg ataatcagat tatcgttgtt tttctgataa ataaaccaat
     6061 tgtttacttt attatcttcc actttagtgt aataatttac tgctgaaaaa tataccggtt
     6121 caccatcttt cagattaaaa cttactacag tttcatcatt tacaggcgga tagaaagtta
     6181 ctccttcgat tgctgtttct tcccgccagt tcccggcaag tgttattgta attgatgaag
     6241 gagaacttac ccaaccaaaa atcggatcgg taacaggctt taaatttgga ttacataccg
     6301 caaaggataa tttattatct actatgttct ctttctgcaa taaaagtaaa gacgaactgc
     6361 agctttttat tttgccgaat gaaatattgc tcacagcatc aaaaaatgca taagcattga
     6421 tttgtgcagg caaatatatg atagagtgga gtgttgatgt ctgcgaattt attttgtaga
     6481 tgcttcctcc gttattttgc aattgagcag tcattgcttg cattgcagtc gtgtttgttc
     6541 ccggaactac aacaaatgag taatttttag atgtgggctt aactccatga tttaaataag
     6601 ctttggcagc atttagtgtt gtaaccggtg aagcataatc agaaccatct tctttcgggg
     6661 tactttgcgc atcgtatttt acaacaatgt catcgtttcc ctttggaata aaatatccgg
     6721 ttcccgtagg tgttacaaac cagttatctg ttgttgaaga taatgttgta ttatatggtt
     6781 ttgtaacatt atttccgttt acaacaaaat taccatttac gcctgtaaca atattctgaa
     6841 acaaattagt agaagtaaac caggccgaat tataggttcc gcttgaactg atgttgcttc
     6901 ccaaacttat taccattccg tcaaaagcaa acatagactt tttgaaagta agatttgtcg
     6961 gtgtaaaccg ttgacttccc caggaatcta tttgatcaaa atcggcagca aacattccgc
     7021 aatcgcccca tgacaatgca cctgcaaaat ctttcgtttt ggtatattgg tcaaaccgat
     7081 cggttaagtt tctgttgggc atcaaatcct gccaggtgtt atactgaaca actgtagcgc
     7141 cttgtggcat attccagtcc caaccaccgc cggtgttgtt tgtgggctct ccgctgtaag
     7201 ccagcgatga accatcgtac aacacttcga gtgtgccatg actttgataa cgcccaaaac
     7261 gattagcatt gctgtaaatt tcagcacccc agaatttcgt tgtaggtgag cgcatagtaa
     7321 ctacccagtt attctttcga taaataccaa tcggactata attgaactga tagaaacctt
     7381 cctggtttga cgtgggaacg ttataaaaat tcgttttata gaaataatta tacttacccg
     7441 ccagttcctc atccattgca gtacccatgc agtcgccgcc aatacttatc aggttttgaa
     7501 acaatgtttt gttgaagtat aatttctggc cacttacaaa aggattgcgg cctgctaaag
     7561 aattggcaaa gaagtgattc tcattaacat cattggtagc catccgatac acagaaacaa
     7621 cagctttttt tattctatta tatgcttcag gtgaaatgcg gtattgggta ccctttaaat
     7681 aattcatgta ttccacccat gtttggtaac aatacatgta attattgtaa tgtgtattgt
     7741 gatgaaaacc tgttccatcc ggtttcagta tatcatttcc tccaggaata tattctgtat
     7801 ttcgttcaat aaaacgtctg aatgctttca attcacgtac ggcagtatta tcatcagggt
     7861 aaaaaatagc acttgccatg atgtgaggaa gataaagata aataatatct gaaacaagac
     7921 tggttttgta aatactttcc ggataataca tgttgccata aaaagtaaac cagcgaacca
     7981 atttcaataa ttctactttt tgttcaggcg tacaggcatt tattgcattt aataatctgg
     8041 ccgggatatc acgtgaaggt gtgtaattac tgctggcaat agcaaaacta catccttctg
     8101 caattccctg atctaaaata tggtcgagat aatttgaaaa acgggtcgtt attgttgaat
     8161 ttgttggatt tttcaaataa tccgaagcta atacttccag tcttactgca atatttgtta
     8221 ttgagtcgat ggttagatac tcaggaaagt tgttaataac tttaccttta acacttccat
     8281 ccgtatttcg ctgtatattc aatccatcaa catagctacg cgcttgaacc aaaactgtta
     8341 aagttggaga agcaggtact cttacaagtt tgttcctcaa taaattcaaa tcggctattt
     8401 cctgagatga tgccgcttgt accggaatgt ctttgtcgaa attgttgatt gtgacaggtt
     8461 cagtattacc tgaaaagtaa tttttatcca atacccattg attcccgact actctgtcaa
     8521 ccgcaactgt ggtgttgaaa tccactttgt caaaatatat ttttctgact ttagtatcag
     8581 tccttttaag agtaaacttt actgatacaa tgtcctgcga actgttagaa gtggaaaatt
     8641 ccgtatattt tctgctaaaa tcacgccatc ctttgtagtt tattaaatag aaagcttttc
     8701 ttctcaataa attaagcgca ctgtaatact caattattaa tgtgtcgttg gttacctcag
     8761 gtgagtataa ctgtaaacgg gtgccattcg aagtattggt caggaaactt acaaaattta
     8821 ccctgagtga gttattccca tttgtaatcc aacaaagact ctgattccca tctttaaaat
     8881 gttcggtcgt aacagataaa acgtcgcctc ctgatgcaat ccagtcggtt ggtgtttcgg
     8941 gttcttcaaa cgaatagttt tgtcctgaaa caaaagtagt cagagtgata aataatatga
     9001 atattacatt ccttttcatg gtcataatta cagtcttgct ggttgcccta atttacagta
     9061 atttcaaatt gacgtattag ctcgtaagat gattcaaagt ttgcattttt agccaggaac
     9121 gtgacaatat atttaccttt tttattgaat gtgtaattat aagcatccaa gtccatcgac
     9181 atttttttaa tagctactcc tgtgtctgct agcctgctgt taattatcag aggatttgaa
     9241 attacccaat cgtcgttgat tgaagaaccg gaaggcgatg actgtattct tatttcagtt
     9301 gaaatgtttg acagattcca aatacccgag ccgcctgtgt ttttgtacgg tgccgttaat
     9361 gcaagcatat ccaaagcctg aaatcccatg tttgatgcct tgattgcaga aacagctccg
     9421 gaagatttca tagtgttttc tatctgcaaa ccggttatta tccatgttgg ttgagtgctc
     9481 gtattttgat ttgtaatgta ttggaaagca acggtaagtt ttttccccat ataatcattc
     9541 aatggtagtg ctacatccac tgtaccatta cttagtgttg gtaaattgca tgacgcggat
     9601 atatcagtcc attgattttt tatcacagcc aaagaatcag tcagtttatc gtatccgcgt
     9661 aatccttcga atgttgttga taagtacacc ttcagtgtac cttcaataat accatattgt
     9721 ggctttgcat taaacttcaa tgtggaagtg atttcttcgg cgggagtttc caccaggttt
     9781 ctttttacat attcatgacc tgattctccg ctgtaaaatg tgataaaatc gggatcaccg
     9841 gtgaaattaa aagtgattgt ttttccggaa tcaactacaa atgcatcacc tgatatcgtt
     9901 ccttcgtcag ttgatgctgt aacattaaat tttacaggtt gtttgagtgc atcttcacaa
     9961 ctccataata cgaagcaacc aatgagaatg gttaatattt tatttttcat tattatcttt
    10021 tattttatgg atttatcggt tatatctaat caatatttct ctactccggc taattaccag
    10081 ccgggattat tagaggtgat ttctttgttt actgatagct cgatacttgg aatcgggtag
    10141 taattataat tttcacttac attgtaataa ctggctgcgt atttgaagcc tgagccccaa
    10201 cctggatcgt taactgaact gcgcatcgac tgcatgtttt tatagaagtc accccagcgg
    10261 attaaatccc aacgtcgaat gccttcaaaa catagttcca tagcgcgttc tttttttaca
    10321 gcatctctaa aagtcgtttg ttccagtgag cctacctgat actcaacaat gtgtgctctg
    10381 cgcctgactt cgttgataca gtcataagcc aattgagtag gagcactatt aatctcgttt
    10441 tcagcttcag caatcatcag taatacatca ctgtatcgaa gtatcgggaa atttataggt
    10501 gtgtaattct tgttttttat caaaacggtt tctaattcgc gccgatattt tgcgcagcac
    10561 cgtgttttgt tattattgct tgccgattga gtcatgtcag tacaaggaag tccttccaca
    10621 gtagccaaac cagcacgtcg ttttccgaaa taataattac gtccggttac ttgttttggt
    10681 gcagtacttg cgtaaacata ttcgtaagga gtaatattcc aatcacagcg ggctgtgtcg
    10741 ttttcgcttt catacatgtt gaaaagtttc agcgaggcat atacaaattt atagctataa
    10801 cccggattgc tcattcctcc ctggttctgg taaggactta ctgtggaata atcgttggta
    10861 ctaccaaatc caatcacatt tccgatacga ccggcggacc aatcaggtga attaattctg
    10921 tttccggctt cttctgcttc ccagatactt tctttctttc cggtactgtt gtatttatcc
    10981 tggcataaat cggtaaagac ctgactataa cttttggtta attcgtgaat tccactttcc
    11041 tttacctcca aagcccattt ctttgcttcc tgaaaatact tttgaacatg gttgtctgcc
    11101 gttttattgt ctctgaaatg ttctcccgca cgaaacatgt aaatacgtgc cagaatgccc
    11161 tttgctgccg actgtgtgac ataacccgga ttggtatttg ctgctgaatt taagtacggt
    11221 attgatttta caatctgatt tgtaatactg tcataaataa tctgtttgtc agtccgtgga
    11281 agcgacagat tatcgacact ttgtgctgaa gtaagacgaa acggaacatc accccatccc
    11341 tgtatcaaat tgaaataata aaatgcgcga agaaaaacgg cttcagattt tactctattc
    11401 ctgattgcct ccgaaatggt ctcattctta tcaacattct caatcaggat gtttgccctg
    11461 cttatcccgt cgtacagaag tcgccagtat gcggaaatat attggtttgt gctgttagcg
    11521 tcagctgtca taatgcttac aggggaatta gcccgttgat aaaagcttaa atcatcacct
    11581 ccggcgttat ataccggata ataattgcca tagaaatatt cctgacccag tgggctgtaa
    11641 actcctgcca gaaaagactg taactcagat tcactgttga aatatgtttc aggagtaagc
    11701 tcgtgtggtt gtttgtcaag aaactcgcac gatgaaatag taagtgtgag taaacttgca
    11761 ataaatattt taatatatgt tttcatctta atttcggtat ttaattatag tgtcagatta
    11821 actccaaaat ttgcagaagc agctcttggg tatgcggaaa aatccaggtt gggtgtcaga
    11881 gctttttctc tgattgaaac ttcgggatca tatccggaat aattatccca ggtgaacaaa
    11941 ttctgagcag agagatacac tcttaacgaa ttaattttta attctttaat cagttttttt
    12001 ggaaaattgt atcccaatga taaggttttc agacggaggt acgaaccatc ttcaataatt
    12061 ctgcttgaat acagaagatt ggatgatgaa ttgttcactc tcggaatatc actgtcagga
    12121 ttgtcaaaag tccaacgatc ggcatagctt gcaaactgat ttagatcttt acgtttagca
    12181 aatgaagttt caaacatcaa tcggttggca ttgatgatat cagcaccata agaccattga
    12241 aagaatatgc ttaaatcaaa attatgatac gaaaagttgt ttgtaaatcc accaacgtga
    12301 ataggatcgc cacgacctat aaatgtttga tcatcgctgt tgataatgcc atcgtcattc
    12361 atatctttat attttggata tcccggttgt gtgttattct cggcagtata acgtgcaatg
    12421 cccggtttta acgagtaatt tccgccaacg acattaaatt catccagttt gtaggtcccg
    12481 tcatacaggt atccatacat cataccgata ggataaccta cttttgcaat ataattgtct
    12541 ccaccaaagt tacggtcgac aatttctgca gtgatatagg atctctgacc ttcattcagg
    12601 ccaagcagta tgtttttgtt gaatgaaata ttaaagcttg aattccagct aaatctctta
    12661 ttcttgatgt ttgtcgtgtt aagggtgaac tcgagtcctt catttcgtac ttttccgata
    12721 tttttcattg cgctgccaaa tcctgaagat attggtaaag tggctttaat cagaagatcg
    12781 taggtgagtt tgttgtaata atctattgtg gtagaaattc tttcgtttag aaataccaaa
    12841 tccaaacctg cattccactg acttgtggtt tcccatttca gattgttgtt agcaaggctt
    12901 acaggaatag ttcctttgat gatggagttg ttaaaagagt aaacgccatg tgtgtaacca
    12961 gagggatcgt tgtatgaacc gtttccggca gactcctgtg ataaacgcgc aaaccttgca
    13021 tattcttcaa ctctgttatt gccggttacg ccccagccta accgaagttt accggatgtc
    13081 agtgcttttt tgaaatccct catgaatttc tcttctgtaa atgtccaggc aactgctgtt
    13141 gatggaaaat atctgaaccg gttttgtggg cttaattttg atgatccgtc agctctgaat
    13201 gaggctgtaa aataatactt ggaaagataa ttgtaattta ctctgcccaa aaacgacatc
    13261 agactccatt cgctttcccc tgaatcattc gtattcggag ttccctgata catacctgcc
    13321 attcccaatg attcgttagg aatgttcgta gttttcatcg tgtagaattt gaaatcgttt
    13381 tcctgtagag taaaaccacc caacatattg atagagtgtt ttttattgat tagtttctga
    13441 taagttagta tgttctcgtt caaccaggta taactttggg atgcaccaac tgaagcattt
    13501 actttatcga ttgatagctg agatccgtac cgtgtgtttg aattgttgaa tacttccagc
    13561 tttcttacat cggtggtgta accgcccgaa accttgagtt tcaatccttt tataaactca
    13621 tattcagcaa aaccgttgaa ttgagtgtag gcgatattgt ttttacggta ttcattattc
    13681 aacgataaat acggattaaa ccggtagtcg atggaagtat cgacattgga atctaatcca
    13741 ttattgataa gtgagttcaa aggaactccc ggttgggtca ccggacgata accccaaacg
    13801 ctgtaaaaga ggttattcat tccgcttgat gctgtttgtg aaggtgaatt acctgtctgt
    13861 gtagagtttg aataatttac acttaagttt atccgtaatt tgttccttct gatatcagtg
    13921 ccaagttttc cctgcaatct tttataatta gattcaatca caataccatc ctggtcgtaa
    13981 taggaaaagg atgcattgta tctgttatca gctgcacctc cggtcagcga cagactgtgg
    14041 ttttgttgca ttgcatcatg aaatatcaaa tcctgccagt tgtattgttc tacgttggca
    14101 taatcagcca gggtgtatgt tttattgtca atggttttgt aataaccggt actgctcatt
    14161 tcggcaggat tccaaacttc ggattgtaat ctgacaaatt cataagcatt cattaaggga
    14221 atcgtacggg taatacgttg taaaccataa cttccatcat aatttatatt tagttgacca
    14281 gttttgcctt tttttgtcgt gatgattata acgccatttg caccgcgtgc accgtagata
    14341 gcagttgccg aagcatcttt cagcacatcg atagattcga tatcatttgg attgatagaa
    14401 gcgcctgctt tcggatcttc taccggaaaa ccgtcaataa catacaacgg agagttatcc
    14461 tgagttacgg agtttttacc ccggataacg atgttcatat tattgccggg tgaaccttcg
    14521 gatgatgaaa cattgacgcc ggcaacccga cctgctaatg cctggtcgaa agttggtaca
    14581 ggagctttca gcatatcttc aacgtttgct ttggcaacag aacctgtaat atctttacgt
    14641 ttcatatcct gataacccac aacaacaact tcattgagaa gttttgagtt atctgtcaat
    14701 gtaaccaaca ttggtttgtt tctctcaact gttttctcaa ttgtttcgta accgatagat
    14761 gaaaatatca atacatcagt tggtttactc acattgatac caaattttcc gttaatatct
    14821 gtaacggtgc cctgagttgt gcctttcaat cgtacggtta cgccaatgag ggtagctccg
    14881 gtttcgtcaa ctactgttcc tgtaatgttt atctgggcaa aaatgccaac agaaaatatc
    14941 aagaaaagta atagtaagtg aattcttagt tttttcatga atatttatta atttgttatt
    15001 tacaatataa taatggctgg taacttcctt actgattaac aaaaaaatga ttaagccgct
    15061 aaaaagttga acttattttt atttttcagt tattcaacca ctactttttt agccagatta
    15121 tttatccgga cgatatatat acctttttct actggaacta taaattgagg tgtgtcggct
    15181 tttttgttga agattaaatt accagttatt gtgtatacac taactttgtc attctggtta
    15241 atttttgaga atataatctt gtttttggta gcaaatacac tataaacata atcaatattt
    15301 tccagtcccg aagttgacga ccatacggca tacagcttca atgattttac caacggatgt
    15361 gaatgccaaa tacctgcagc atctattttg tcagtaatat ctacctcctg accatcgaat
    15421 gaagttaaat catcactacc tgtaatgtaa gcagctttca atagtttata acctggtttt
    15481 gcttcgaaca aaaagtccga aacagttcct tccgggtaac ccagaattgt tccgtcaggt
    15541 aattgcgtag catttgaatc ataaacagtt ccgttggtat tcgtgccttc accaataagc
    15601 attttacggt aaggtaattc gtaagctccc atatcatatt gtgctgatgc agttggatga
    15661 ccttgcggac gttgtacacc caaataatct aaatttgttg caactaatgt tttaccaata
    15721 ttcaaacagg gtgaattgta tttaattctc cagtctgccg agtctaatga tagaatttct
    15781 tcatcagctg cagctgtacc cctgaaacgg gttacatgat agaattttgg tccggaacca
    15841 actgttcccg gtgcaggatc tgtaacatct ccgttgctta tattggaaga aaattgaata
    15901 tttcccgacg ttttccaatt cttgtcagtt ggtatcgggt tatatgtggc attattgttt
    15961 attgtagcat ttgcatttgc agctccgctt aaactggtgg cagttgtttc agtaatatct
    16021 gtatacatgc cccagaaaat attgttactc aattctatag ttgcagatgc ttcggctaag
    16081 tatgcacctc cgatattgtt gaccatcgtg cagttaaata catttccttt tatatagaat
    16141 gttgttaagc cgctgttgtt tgatattata caattcgtag ctgtattggt tgcactgttg
    16201 gcataaatcg cagcgcctgc ttttggtgtt cctgaatctt ctgatttgtt atttacaatg
    16261 tataaatttt tcaggtacgt gaatccaaca ccttgtacgt tgataccacc accacgaatg
    16321 gtagaggagt ttttttcaat tttacaattt tcaaccgttg tttcatttcc ggatgcggtg
    16381 ttaatgtata ttccacctcc gccatttgca tttgttgttt gtctgttgtt gtaaatccag
    16441 ctattcttta cagttcctcc tgtcatgttc aatcctccac cggcaccatc caatgctgtt
    16501 gaattttgaa ttacgcagtt ctgtatgacc atgttgtttc ttaccacagc tgctcctccc
    16561 gatttactca gagtggttcc aataccattt ccattcgtca aaataaaacc atccactatg
    16621 gtagccatat cgaagtttga agcagtttca atcaaccggc aattatttgc agtcaatatc
    16681 gtcgaattgg taaattccca gggtttgccg tttgtcaatt ttgtacgttg atctacagct
    16741 gtttctgtac cagcaaaact tccatagaga cttatactgt cagacattac aattgctgta
    16801 cctatcgtat aatcccctgc agctacccac acatccttac gtgcagcttt atcagcacga
    16861 gctgtgttaa tggctgtttg aatatctccc atagcatttg cccatgaagt accatcgcct
    16921 gcgccacccg gttttacata cacaataccg tttttctgtt ctgccattgc tgtcatggat
    16981 aacagtacaa ctaatgataa taataatttt ttcatattaa ttaaatttga tagttttagt
    17041 taggataaat ccagacacag attttggctg tgtctggatt actgtgtgat tttttataga
    17101 tagattattt aacaatgact tttgttgcct gatcagccgc gcgtacaatg taaatgcctt
    17161 ttgatacatg tagcgagata ttggatgcat ttgcattgac ttcttttact ttagctccca
    17221 atgcattgaa tactattact ttctcaccga tatttattcc ttctattgtt atttgattac
    17281 ttgacgtaaa gcatttaaaa gtacttttca gatttgacaa gtgggtaccc ggatctgttt
    17341 caaatgtgac attcagagtc gaaattcctg ttagtgccgg agcggtgtat acattttcag
    17401 aaagttgtga tgttacatct gtaccgttat atgaaacagc agaaatttta taacctgaat
    17461 tgggagtaat cgtataattc attgcgatcc ctttaggttg agcgatgata tctcctgatg
    17521 tataagaatt gactgttcca cctgtgttaa atgtaactgt aatgttgcaa taaggaagtt
    17581 cataagcgcc aatgtcataa gcacttccct gcggacgggc aagacctaaa aaatcagtag
    17641 tgggaacacc agcaattgag ttgcttgcag cattaagtag tggggattta tacgtaatag
    17701 tccagtcatt attcaatgga tctgcaaaat agggatagtt cgtttctgaa atcaatgtat
    17761 ctaattggat attgtcagct acagtccagt ttgggaatgt cgggattgta atcgatgcgt
    17821 tgtttttgta ttcaactccg gccacatcta cccctgatac tgttgctttt gatttgtttt
    17881 tggcccaaaa cacactattg taaatttgtg cgtcttttgt tgcagtataa acaccacctc
    17941 cctcattgtt tactattgtc gaattataaa cagctgctcc tcttaaatat aatgcagact
    18001 tggttttatt gccgtaaaaa acgcagttga taattttatt ggctttgtct gtgtcaacct
    18061 gagtgtaaat agcactgcca t
//
LOCUS       DAOPYB010000046        18020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3265_length_18020_cov_9.153131, whole genome shotgun sequence.
ACCESSION   DAOPYB010000046 DAOPYB010000000
VERSION     DAOPYB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18020
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3265_length_18020_cov_9.153131"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..694
                     /locus_tag="PKH58_05295"
     CDS             116..694
                     /locus_tag="PKH58_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNX88474.1"
                     /translation="MMKDLKGTQTEQNLLKSFAGESQARSRYTMFASIAKKEGFEQIA
                     AIFLETAEQELTHAKKFFRYLQGGMVEITASYPAGVEGTTAENLKAAADGENEEWVDL
                     YPHFADIAEAEGFDLIAATWRKIAKVEAEHEKRYRKLLERVETNTVFAREEETTWQCR
                     ECGYVHTGKEAPKACPVCAHPQAFFEEEKNNY"
     gene            784..1446
                     /gene="mnmD"
                     /locus_tag="PKH58_05300"
     CDS             784..1446
                     /gene="mnmD"
                     /locus_tag="PKH58_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNX88475.1"
                     /translation="MQKPIIKTTADGSDTLYVPEIDESYHSTNGAVQESMHIFINDGL
                     RSCTKKSIKVLEIGFGTGLNAFLTLLEAENANKTIFYTALELYPVDVSEAIKLNYPEW
                     VDNTKKALFENMHYSQWNEIVEITDHFTLIKLLTDFTIADFSEKFDVIYFDAFSPEKQ
                     PEMWSEELFRKLYDHAEEGAILTTYCAKGIVRRAMQSAGFSVERLPGPAGKREILRAR
                     KL"
     gene            1449..2111
                     /locus_tag="PKH58_05305"
     CDS             1449..2111
                     /locus_tag="PKH58_05305"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX88476.1"
                     /translation="MKRENYHNIQYFLAPYFTLALGLLLLIIIVPKEELHLMMTEHRS
                     VVADCFFRIFTEIGGLIPFIVVLILLFYRYSTSLFLALSQLTGLLTAFIVKNIFHSPR
                     PSLYFKEFYPEILLPVVPGFDLMTYNSFPSGHTVNAFALFFCIALITKNKWNKLLCFV
                     IAALVGYSRIYLSQHFAIDVLPGSLIGIISAWMVHPYYLKFDQKWKNKSLSVHFQQKK
                     KQ"
     gene            2215..2490
                     /gene="rpsO"
                     /locus_tag="PKH58_05310"
     CDS             2215..2490
                     /gene="rpsO"
                     /locus_tag="PKH58_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNX88477.1"
                     /translation="MYLTSEKKQELFKAHGKLKSATDTGSPESQIALFTFRINHLTEH
                     LKVNKKDFATERALTMLVGKRRRLLDYAKKKDINRYRSIIKELGIRK"
     gene            2585..3946
                     /gene="hisS"
                     /locus_tag="PKH58_05315"
     CDS             2585..3946
                     /gene="hisS"
                     /locus_tag="PKH58_05315"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNX88478.1"
                     /translation="MQTPSIPKGTRDFTPREMANRNYIFNTIKDVFRLYGFQQIETPA
                     MENLSTLMGKYGEEGDKLLFKILNSGDFLSGVDDREMSEKNSLKLTNKISEKGLRYDL
                     TVPFARFVVQHRNELSFPFKRYQIQPVWRADRPQKGRYREFYQCDVDVVGSNSLLNEL
                     ELIQIVDEVYRRLKINVVLKINNRKILSGIAEIVGETERITDITVAIDKLDKIGLENV
                     NKEMAEKGISDENIAKIQPILKLSGTNQEKLNALKTILSGSATGLKGVEEMETIFNLC
                     ESVKIKTAVELDLTLARGLNYYTGAIFEVKALDVQIGSITGGGRYDNLTGVFGMEGVS
                     GVGISFGADRIYDVLNQLNLYPETSEEQTRILFVSFGEKELNYCLPWTNELRKQDINV
                     EIYPEPAKMKKQMSYADNKGIPFVAIVGETEMNENKVMLKNMKTGEQNLVTQEDLITI
                     IKM"
     gene            complement(3986..6718)
                     /gene="metH"
                     /locus_tag="PKH58_05320"
     CDS             complement(3986..6718)
                     /gene="metH"
                     /locus_tag="PKH58_05320"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNX88479.1"
                     /translation="MKLRSELENRILILDGAMGTMIQRHRLDEKAYRGERFAGSNILQ
                     KGNNDLLVLTQPEIIYDIHCLYLQAGADIIETNTFNAQRISMEDYGMGDFVREINLEA
                     VRLARKAAEEFTVKNPSKPRFVAGSVGPTNKTASMSPDVNNPAYRAVSFDDLVEAYKE
                     QILALIEGGVDALLIETIFDTLNAKAALFAAEETMNLLGKRVELMLSATIADKSGRTL
                     SGQTIGAFLTSVSHADILSVGLNCSFGARDLKPYLQEMSSRCGVYVSAYPNAGLPNQF
                     GEYDETPDKMAVQVKEYIDEQLVNIIGGCCGTTPDHIATYVKLTENASIRRPVKKDVN
                     LHLSGLEELVVNESSLFLNIGERCNVAGSRIFLRLINEKKYDEALLVARKQVEDGAQV
                     IDINMDDAMLDARQEMITFLNYIASEPEISKVPVMIDSSKWEVIEAGLKCVQGKCIVN
                     SISLKEGEEDFLRKARKIRQYGAAAVVMAFDENGQADSFERKTAICSRAYYLLTEKAG
                     FPPQDIIFDPNILAIATGIEEHNNYGVDFINAVRWIKQNLPYAKVSGGVSNLSFSFRG
                     NNIVREAIHSVFLYHAIRAGLDMGIVNAGMLQIYEDIPAEMLLHVEDIVLNRRQDATE
                     RMIELAEQVKNNSSDNKQEQVDAWRTEPLQKRLEYALVKGIDQYLEEDLREALLFYTS
                     AIEIIENPLMSGMNQVGELFGAGKMFLPQVVKTARTMKKAVAILQPEIEKYKVQGQST
                     SAGKYLFATVKGDVHDIGKNICAVVLACNNFEVIDLGVMTPTEKIIQTAIDEKVDIIG
                     LSGLITPSLEEMSNVAAEMEKAGLKIPLMIGGATTSKIHTAVKIAPKYSGLVIHGKDA
                     SQNSSIAMQLMNDVSNKKIKEGIAAEYQAIRDKQVPKSDLLSLDEAKKRKPKLF"
     gene            complement(6818..8344)
                     /gene="gpmI"
                     /locus_tag="PKH58_05325"
     CDS             complement(6818..8344)
                     /gene="gpmI"
                     /locus_tag="PKH58_05325"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNX88480.1"
                     /translation="MRKKALLMILDGWGIGNHSKADIIYNTPTPYWDSLLATYPNSYL
                     LASGENVGLPDGQMGNSEVGHLNIGAGRVVYQDLVKINRACRDNSILQNPEIVNAYSY
                     ARDNKKKIHFLGLVSDGGVHSSLDHLLKLIDIAKEYGISDAYVHCFMDGRDTDPKSGK
                     GFIEQLQQHTEKSTGKIASIIGRYYAMDRDKRWERVKLAYDLLVKGKGTPATDMVAAM
                     QNSYDDGVTDEFIKPIVGVDSNGNPLAKIEAGDVVIFFNYRNDRAKELTIVLTQEDMN
                     EEDMHTIPLEYYCMTPYDSSFKGLHVLFDKDNVENTLGEYVSALGLKQLRIAETEKFA
                     HVTFFFSGGREAEFPGEERILVPSPKVATYDLQPEMSAPEVAVKLVDALNTQKFDFIA
                     LNFANGDMVGHTGVREAIVKAVETIDVCVSKVVEAAKANDYEVIIIADHGNADNAENE
                     DGSPNTAHSLNPVPFVYVTENKDAKVENGILADVAPSICQILGIEKPKEMTGKGLLTI
                     "
     gene            8516..8788
                     /locus_tag="PKH58_05330"
     CDS             8516..8788
                     /locus_tag="PKH58_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HNX88481.1"
                     /translation="MFKVIACMIAGIGLGFVLRNHKIRFIQGFIITLIWLLLFSLGLE
                     IGSDKNVIAQFGKLGFEAFILASMGTLGSVFFAKLLMNFIQKKQKK"
     gene            complement(8804..9517)
                     /locus_tag="PKH58_05335"
     CDS             complement(8804..9517)
                     /locus_tag="PKH58_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNX88482.1"
                     /translation="MSNPYFRFKQFTVFHDKCAMKVGADGVTLGAWADITNAKKMLDI
                     GCGSGLIALMVAQRSNAEITAIDIDNQSVIQSTINVENSPWKERIIIRQISLQDFAEC
                     TSEKFDVIISNPPFFRNSLKNPSQGRTVARHADTLPHEQLLICSKKLLNPGGRLCIIL
                     PIAEGEDCVKTGKEAGLFCSRKVNVFPNTVKQPKRLLLEFVMTETACRKSNLTIETEI
                     RNQYTPEYTELVKDFYLKL"
     gene            complement(9526..10119)
                     /locus_tag="PKH58_05340"
     CDS             complement(9526..10119)
                     /locus_tag="PKH58_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88483.1"
                     /translation="MLVVAQSTAVTDRTPEQEALKQTEKLQNELQLTEQQYKAIYQIN
                     LRYARERQQSNTRSQAMERIRNKNEDIQKVLSSKQYDELQNRRNEVKVVEIDNANRYV
                     RTNPQTRVNSSDQRTGRTGTGNTDFNTDVRSTSRTDNRERTITRQYGTQREVQTRTRS
                     VTPTRSSDAGSVRSNSGRTSESRSESRSESRTSSGRR"
     gene            complement(10300..11067)
                     /locus_tag="PKH58_05345"
     CDS             complement(10300..11067)
                     /locus_tag="PKH58_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNX88484.1"
                     /translation="MKLNLKNPIVFFDLETTGTNIVSDRIIEISYLKVSPNGREETKT
                     IRVNPGMHIPESSSAIHGIYDEDVKNCPVFKEVAKEIIHDIEGCDLAGYNSNRFDIPL
                     LAEELLRADVDIDLMKRKFVDVQVIFHKMEQRTLSAAYKFYCSKDLENAHSAEADTKA
                     TYEVLKSQLDMYPDLKNDVEFLSKFTAQTNNVDFAGRIIYNDKGEEIINFGKYKGQKV
                     SDVLKTDIGYYGWIMNSDFTLHTKKVLTNIKLRDFQK"
     gene            complement(11163..12299)
                     /gene="dnaN"
                     /locus_tag="PKH58_05350"
     CDS             complement(11163..12299)
                     /gene="dnaN"
                     /locus_tag="PKH58_05350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNX88485.1"
                     /translation="MKFVVSSTELLSHLQAISRVINSKNSLQILDNFLLSLEGNTLTM
                     TASDIETTLITSMEVQDVVGSGKVAVANKLLLDTLREFSDQPLAFQIDDSNLAMVITS
                     SNGTYNFIGQSGDMYPQLPVLEENARQLNVSVPVLLSGISKTFFCMADDELRPVMNGI
                     FFDITSENLTLVATDAHKLVRFKSAYLSASISGDEALSFILPKKPAIMLRTILPKEMG
                     EVEIKFDEKNAYFQLSNYTMVCRQVEGRYPNYNGVIPKNNPFKVIADRTTLLNALKRV
                     SVFSNQASNLIKLEFQQNQIHISAQDIDFSISAEEYIPCQYEGEYIKIGFKSTFLVEM
                     LGNVDANDIILELADPSRAGILLPLENAENEDILMLLMPMLLND"
     gene            12513..13049
                     /gene="hpt"
                     /locus_tag="PKH58_05355"
     CDS             12513..13049
                     /gene="hpt"
                     /locus_tag="PKH58_05355"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNX88486.1"
                     /translation="MKTIQIKDKQFTLSIPSEEILEAVKKVGDAINKDLADKNPLFIC
                     VLNGAFMFAGDLMKVVEIPCEITFIKLSSYEGLITTGIVKEVIGLNESVVGRNVVVVE
                     DIVDTGITMDKILTSLQAKGANEIYVATFLQKPAALKRDVKIDYVAMKIPNEFIIGYG
                     LDYDGYGRNLKDIYTVVD"
     gene            13101..13679
                     /locus_tag="PKH58_05360"
     CDS             13101..13679
                     /locus_tag="PKH58_05360"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNX88487.1"
                     /translation="MINLIICGAPGCGKGTQSDLIVRKYNLTHISTGDLLRKEIADNT
                     ELGKVAYQYISKGQLVPDQMIIDILANKLNSLQNSNTNGIILDGFPRTLAQAEALEKL
                     FSERDTQTDILLDLQVDEDELIARLLKRGETSGRSDDNLETIKKRLDVYYNQTKPVSD
                     YYQSLGKYAGIYGMGTIDEIFGRISETIDAKL"
     gene            13807..14946
                     /gene="obgE"
                     /locus_tag="PKH58_05365"
     CDS             13807..14946
                     /gene="obgE"
                     /locus_tag="PKH58_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNX88488.1"
                     /translation="MAGSNFIDYVKIFCRSGKGGAGSLHFHREKFIAKGGPDGGDGGR
                     GGHIILKGNRNLWTLLHLKYARHIYAGDGGDGSKNKSFGKDGKDQTIEVPCGTVVYDG
                     ETGEFLCDITHDGEEVVLMNGGRGGLGNWHFRTATNQAPRYAQPGEPRIEKTIIMQLK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLAPNLGIVSYRDNKSFVMADIP
                     GIIEGASEGKGLGLRFLRHIERNSILLFMVPADTEDIKKEYQILLNELQKYNPELIDK
                     QRILAISKCDMLDEVLIEQMKKELPDDVNTLFISSVSGTGITELKDVLWEELNKDTNK
                     IVDITHKPIEVTGMLTEEDEMEIEVDEFDDEDDLSKYKGIGWDEQ"
     gene            14936..15736
                     /gene="pgeF"
                     /locus_tag="PKH58_05370"
     CDS             14936..15736
                     /gene="pgeF"
                     /locus_tag="PKH58_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNX88489.1"
                     /translation="MSNNFESGFIQYPVLNRYDTIRHFTTTRMGGVSTGNYSSFNLGL
                     FSGDNPDDLSKNFKLLSTLTNIPEDNIHLPYQTHSNKISLIDEDFLSKDNETRHAMLQ
                     GVDALITNIPNQCIGVSTADCVPVLLYDPVQHVVAAAHAGWRGTCSKILTRVITSMKA
                     AYNTNPQNLIAVIGPSISPEVYEVGISLLEKFGDAGFNIRKIFIHKNGKIYLDLWTAN
                     KTLLTNAGVPEENIEISGICTFSNHEKFFSARRLGIYSGRMISGIMLV"
     gene            complement(15739..15939)
                     /locus_tag="PKH58_05375"
     CDS             complement(15739..15939)
                     /locus_tag="PKH58_05375"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNX88490.1"
                     /translation="MAKKQTYTESFEELNRILAELENMNTEINMEMIEEKVKKAAFLV
                     EKCKKQLHEMDKELEKLLENLE"
     gene            complement(15952..>16796)
                     /locus_tag="PKH58_05380"
     CDS             complement(15952..>16796)
                     /locus_tag="PKH58_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNX88491.1"
                     /translation="FLNFGNSQWILILYLVLIILFKIFASTATTSPGGVGGIFAPSLF
                     IGSITGYVVAVVMNRFGIISPIPNFALAGMSGLMSGVMHAPLTGIFLIAELTGSYNLF
                     MTLMIVSVVSFLTIIIFEPHSLYAMRLAQKGELITHNKDRAVLTLLKMENVIETDFTE
                     VAPSMTLGQLVTAISRSKRNIFPVVEPEKGTMLGVVLLDEVRNIMFRPELYNRFTVAQ
                     LMISPPATIDVNLPMEKVMQIFEDSGAWNLPVLDEQKRYLGFVSKSKIFNSYRRVLVH
                     FSEE"
     assembly_gap    16797..16896
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16897..17885)
                     /locus_tag="PKH58_05385"
     CDS             complement(<16897..17885)
                     /locus_tag="PKH58_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNX88492.1"
                     /translation="MEKKVWYNKMIVWREEHIAHKHFILILSFFVGLFASLAAFILKS
                     SIHFIQHLLTSWYNPNSVSYWYLVFPFIGIILAYLFVRFVVKDDISHGITRILYAISQ
                     RKSIIKLHNTWTSMVGSAVTIGFGGSVGAEAPIVLTGSAIGSNLGKFFKLDQKTLLLM
                     VGCGAAGAIGGIFKAPIAGLVFTLEVLMLDMTMTSIAPLLISSITATSLSYFLTGNEF
                     LFEFTKYEPFSVTRIPQLILLGVICGFVSLYFTRGMNFLEGFFRSVKSPLIKLMLGGI
                     TLSLLIFVFPPLYGEGYDTIEVLLNGNASSVLDRSFFLNFGNSQWILILYLVL"
BASE COUNT         5399 a         3689 c         3423 g         5409 t
ORIGIN      
        1 attaaatatg attaaaaatg tatttatata cctttctaat actggtataa atttcaaaaa
       61 aatgattaga tttgcctctc tatttccagc aacagttata aattaaatat ttatcattat
      121 gaaagactta aaaggtacac agaccgaaca aaacctgctg aaatcatttg ccggtgaaag
      181 ccaggcccgc agtcgttaca cgatgtttgc aagcatcgct aaaaaagaag gttttgaaca
      241 gattgcagct atttttctcg aaacagctga acaagaactg acacacgcga aaaagttctt
      301 tcgttacctg cagggaggca tggtagaaat cacagcttct tatccagccg gagtggaagg
      361 aactactgca gaaaatctga aagccgctgc cgatggtgaa aatgaagaat gggttgatct
      421 ctatcctcat tttgccgata ttgctgaagc tgaaggtttt gatcttattg ctgccacctg
      481 gcgcaaaatt gccaaagtgg aagctgaaca cgaaaaacgt taccgcaaat tactggaacg
      541 tgttgaaact aacactgttt tcgcgcgcga agaagaaaca acctggcaat gccgcgaatg
      601 cggatatgta catacaggta aagaagctcc gaaagcatgt ccggtttgcg ctcacccgca
      661 ggcattcttt gaagaagaaa aaaataacta ttaatcttca ggaaaaataa gaaaaagtga
      721 ccgctgttcg aaaaatgaac agcggttctt ttttcaggaa aaacaactat ttttgtaatt
      781 cctatgcaaa aaccaatcat caagactact gctgacggtt cggatacgct atatgttccc
      841 gaaatcgatg aaagctatca ctccaccaac ggagcagtac aggaatcaat gcatatattt
      901 atcaacgacg gattgcgttc gtgtacaaaa aagagtatta aagtcctgga aataggtttc
      961 ggcacaggat taaatgcctt tctgacgttg cttgaagccg aaaatgcaaa taaaacaatt
     1021 ttttataccg ctctggaact ttaccctgtt gatgtcagtg aagctataaa gctcaactat
     1081 cctgaatggg tggataacac taaaaaagct ttattcgaaa atatgcatta cagccaatgg
     1141 aatgaaattg ttgaaatcac agatcatttc acgctgataa aactgttgac ggattttaca
     1201 atagccgatt tttccgagaa atttgatgta atctattttg atgccttttc gcccgagaag
     1261 caaccggaaa tgtggtctga agaattattt cggaaactct atgaccatgc cgaagaaggt
     1321 gcaatactta ccacttattg tgccaaagga attgtacggc gggcaatgca atcggctggt
     1381 ttcagtgttg agcggcttcc ggggccagca ggcaaaagag aaatattacg tgcccgaaaa
     1441 ttgtgaatat gaaacgtgaa aattaccata acatccaata tttcctggcg ccctacttta
     1501 cgctggcatt gggattgttg ctgttaatta tcattgttcc gaaagaggaa cttcacctga
     1561 tgatgaccga acaccggtct gtagttgccg attgtttttt tcgcatcttt accgaaattg
     1621 gaggattaat tccttttatc gttgtattaa ttcttctttt ttatcgttac agcacctcgc
     1681 tttttctggc tctctcacaa cttaccggac ttttaactgc tttcattgta aaaaatattt
     1741 ttcactcacc ccggccaagc ctttatttta aagaattcta cccggaaatt ctgcttccag
     1801 tcgtgccggg ttttgatttg atgacttaca acagttttcc ttccggacat actgtaaatg
     1861 cttttgcgct atttttctgt attgcactga taaccaaaaa caaatggaac aaattacttt
     1921 gttttgtgat tgcagcactg gttggctact cacgtattta tctatcacag cattttgcca
     1981 tcgatgtact tccgggctca ctaataggta ttatttcggc ctggatggtg catccctact
     2041 acctgaaatt tgaccagaaa tggaaaaaca aatcgctttc ggttcatttc cagcaaaaaa
     2101 agaagcaata atgcaatgac atttcaaaat aaaacattat ctttgcaccc tcaattattg
     2161 gaacatagag tgaaagctct gttcttaatg tacaacccaa aacagttaat tattatgtat
     2221 ttaacatcag aaaaaaaaca ggaacttttc aaagcgcacg gaaaattgaa atcagcaact
     2281 gacacaggtt ctcctgagtc tcagattgca ttatttacct ttcgtatcaa tcatttgacc
     2341 gaacatttga aagtaaacaa aaaagacttc gccacagaaa gagcattgac catgttggta
     2401 gggaaacgcc gtcgtctgct cgattatgcc aagaaaaaag acatcaatcg ttaccgttca
     2461 attatcaaag aattaggtat cagaaagtaa tatttgcttt caaagcaata aaaggcgttt
     2521 caattattca ttgaaatgcc tttttagttt atttttgtag ctgaaaaatt tatacccaca
     2581 ttatatgcaa actccatcca tacccaaagg aacccgcgat ttcactccgc gtgaaatggc
     2641 taaccggaac tatatattta acacaataaa ggacgttttt cgcctctatg gttttcagca
     2701 aattgaaact ccagccatgg aaaacctgtc gaccctgatg ggaaaatatg gcgaagaggg
     2761 cgataaacta ttgtttaaaa ttctcaattc aggcgatttt ctttcaggtg tcgatgacag
     2821 agagatgagc gagaaaaaca gcctgaaact aactaataag atttcagaga aaggcttacg
     2881 ttacgatctg acagtacctt ttgcgcgctt tgtggtgcaa catcgcaacg aactgtcgtt
     2941 ccctttcaag cgttaccaga tacagcccgt gtggcgtgcc gaccggccgc aaaaaggacg
     3001 ttaccgcgaa ttctaccagt gcgatgtgga tgttgtcgga agcaattcgc tgctgaatga
     3061 actggaactg attcaaatcg tagatgaggt ttacagacgt ctgaaaatca acgttgtact
     3121 aaaaattaac aatcgcaaga ttttatcagg aatagctgaa attgtgggtg aaacagagcg
     3181 tattaccgac attaccgttg ctattgataa actggataaa atcggtctgg agaatgtgaa
     3241 taaagaaatg gctgaaaaag gaatttctga tgaaaatatc gctaaaatcc aaccaattct
     3301 aaaactcagc ggaaccaatc aggaaaaact caatgcactg aaaaccattc tatcgggttc
     3361 ggccaccgga ctgaaagggg tggaagaaat ggaaacgatt ttcaatcttt gcgaaagcgt
     3421 gaaaataaaa actgcggtag aactagatct gacactggct cgcggattaa attactatac
     3481 gggcgccatt tttgaagtaa aggcacttga cgtacaaatt ggcagcatta ccggtggcgg
     3541 acgctacgac aacctgacag gtgttttcgg gatggaaggt gtttcgggtg taggaatctc
     3601 atttggtgcg gaccgcatct acgatgttct taaccagctc aatctttatc ccgagacttc
     3661 ggaagagcaa actcggattt tgtttgtttc ctttggcgaa aaagagctta attattgtct
     3721 gccgtggaca aacgagcttc gtaagcaaga catcaatgtg gaaatctacc cggagcctgc
     3781 aaaaatgaaa aaacaaatga gctatgccga caacaaaggt attccattcg ttgccattgt
     3841 gggcgaaacg gagatgaatg aaaataaagt gatgttgaaa aacatgaaga cgggggaaca
     3901 aaacctcgtt acgcaggagg atttgataac tatcatcaaa atgtaaaaga aatgagggac
     3961 atataaaatt tgtccctcat ttcttttaaa acaatttagg tttccttttc ttcgcctcat
     4021 caagcgaaag caagtcagat ttcggtacct gcttatcccg aatagcctga tattcggcag
     4081 ctatgccttc tttaattttt ttatttgaaa catcgttcat caactgcatc gctatggacg
     4141 aattttgtga agcatcttta ccgtgaatga ccagtccgct gtatttgggt gctattttta
     4201 cagcggtatg aattttggag gttgtagcgc cgccaatcat taatggaatt ttcaatcccg
     4261 ctttttccat ttcagcagca acattgctca tttcttctag cgatggcgta atcagtccac
     4321 taagaccgat gatatccact ttttcatcaa tagcagtctg aataattttt tctgtaggtg
     4381 tcatcactcc cagatcaatc acttcaaagt tattgcaggc aaggacaact gcgcagatgt
     4441 tcttaccgat atcatgcaca tcgcctttga cagtagcaaa aaggtatttt ccggccgaag
     4501 tactttgtcc ctgaacttta tatttttcaa tttcaggctg aagtatggca acagcttttt
     4561 tcatcgtacg agcagttttc accacttgcg gcagaaacat ttttccggct ccaaaaagtt
     4621 caccgacctg gttcattccg ctcatgagag ggttttcgat aatctcgatg gctgaggtgt
     4681 aaaatagcaa cgcttctctc agatcttctt ccagatactg atcgatgcct tttaccaacg
     4741 cgtattcaag tcgtttctgc aagggttcag ttcgccatgc atccacttgc tcctgcttgt
     4801 tgtccgatga attgtttttc acctgctcag caagttcaat catgcgctcg gtagcatcct
     4861 gacggcgatt gagtacaata tcttcgacat gcaggagcat ttcggccgga atatcttcat
     4921 aaatctgcaa cattcccgca ttcactattc ccatatcgag accggcacgt attgcatgat
     4981 aaagaaaaac ggaatggatg gcttctctta cgatgttatt ccccctgaac gaaaaggaca
     5041 aattgctcac tcccccgctt actttggcat atggtaaatt ctgttttatc cagcgtacag
     5101 cgtttataaa gtcaacaccg tagttattgt gttcttcaat gcctgtagca atagccagaa
     5161 tattcggatc aaaaataata tcctgaggcg gaaatcctgc cttctcagta agcagatagt
     5221 aggctcggga acagattgcg gtttttcgtt caaaactgtc agcctgacca ttttcatcaa
     5281 aagccataac aacggcagct gcaccatatt gtcggatttt tctggctttt ctcaaaaaat
     5341 cttcttcgcc ttctttcaga ctgatcgaat ttacaatgca ctttccctga acacatttca
     5401 gtccggcttc tattacttcc catttagaac tgtcgatcat tacgggcact ttcgaaattt
     5461 ccggttcaga agcgatataa ttgaggaaag taatcatttc ctgacgggca tcgagcatgg
     5521 catcatccat attgatatct atcacctgcg ccccgtcttc cacttgtttt cgcgcaacca
     5581 ataacgcttc atcatacttt ttttcgttga ttaagcgtaa gaatatgcgt gaaccggcta
     5641 cattgcagcg ttcgccgata ttaagaaaaa gagaactctc atttacaacc aactcctcca
     5701 aaccactcag atgcaaattc acatcctttt ttacaggtct tcgaatgctg gcattttcag
     5761 ttagtttcac atacgttgca atatgatcgg gagttgtacc acaacagcct ccaataatat
     5821 tcaccagttg ctcatcgata tattctttca cctgaacagc cattttatcg ggtgtctcat
     5881 catattctcc aaactgattg ggcaatcctg cattcgggta tgcactcaca tacacaccac
     5941 aacgtgaact catttcctgc aagtaaggtt tcaaatccct ggctccaaac gaacaattca
     6001 aacccaccga aaggatatcg gcatgcgata cagaggtcag aaaagctccg atagtctgtc
     6061 cggataatgt acgaccgctt ttatcggcaa tcgttgcaga caacattagt tcaactcgct
     6121 ttccgagcaa attcatggtt tcttctgcag caaacaaggc tgctttcgca ttaagtgtat
     6181 caaatattgt ttcaataagc aatgcatcta ctccaccctc aatcaatgca agtatctgtt
     6241 ctttgtatgc ttccaccaaa tcgtcgaaac tgacagcacg atatgccgga ttatttacat
     6301 cgggcgacat ggaggcggtt ttattggttg gtccaaccga acctgcaacg aaacgaggtt
     6361 tgctgggatt ttttaccgta aattcctccg cagctttacg agccagccgt actgcttcca
     6421 ggtttatttc acgcacaaaa tcacccattc cgtaatcctc catcgaaatc cgctgagcat
     6481 tgaatgtatt tgtttcaatt atatctgctc ctgcttgcag gtacagacaa tgaatatcgt
     6541 agataatttc gggttgggtt agtaccagca agtcattatt tcctttctgg agaatatttg
     6601 aaccggcaaa ccgttctcca cgatatgctt tttcatccag ccgatgtcgt tgtatcatcg
     6661 tacccatggc accatccaat ataaggattc gattttcgag ctctgatctc agtttcattg
     6721 tttttgaatt aaattcatgc aaagataaag ttatttcctg caaaaaaaaa acgctgcaag
     6781 tgttgactta cagcgttttt tgacttatat catttcgtta aatagtcagc aatccttttc
     6841 ctgtcatttc ttttggtttt tcgatgccca aaatctgaca aatggaaggt gccacatcag
     6901 ccagtatacc gttttctact tttgcatctt tgttttcagt aacataaaca aaaggaaccg
     6961 gattgagtga gtgagctgta tttggtgaac catcttcatt ttcagcatta tcagcatttc
     7021 cgtggtcggc aatgataatc acctcataat cattggcttt ggctgcttca acaaccttac
     7081 ttacgcaaac atcaatggtt tccacagctt tcacaatggc ttcgcgaaca ccggtatgac
     7141 caaccatatc gccattagca aagttgagag caataaaatc gaatttctgt gtattcagtg
     7201 catcaacaag tttaacagct acttcgggag cgctcatttc tggctgtaaa tcgtatgttg
     7261 ctacctttgg tgaaggtaca agaatacgtt cctcacctgg gaattcagct tcgcggccac
     7321 ctgagaagaa gaaagttaca tgagcaaatt tttcggtttc agcaattcgc aattgtttca
     7381 atcccagtgc ggatacatat tcacccagtg tattttccac attatcctta tcaaataaca
     7441 cgtgcaaacc cttgaaactg ctatcgtaag gggtcatgca ataatattcg agtggaatgg
     7501 tgtgcatatc ctcctcgttc atgtcttcct gagttaatac aatagtaagt tctttagccc
     7561 ggtcgttcct gtagttgaag aaaatcacca catcaccggc ttcaattttg gctaatggat
     7621 ttccattgct gtcaacacca acgattggtt tgataaattc atcggtaact ccatcgtcgt
     7681 aagaattctg catggctgca accatatcgg tagccggagt tcctttacct tttaccagca
     7741 ggtcataagc caatttcaca cgttcccagc gtttatcacg atccatggca taataacgac
     7801 caatgatgga agctattttt ccggtagatt tctcggtatg ctgctgcaat tgttcgataa
     7861 atcctttacc acttttaggg tctgtatcgc ggccgtccat aaagcaatgc acataagcat
     7921 ccgaaatgcc gtattctttg gcaatatcaa tcagtttcag cagatggtcc agcgaactat
     7981 gtactccccc atcggaaacc aaaccaagga aatgaatttt ctttttattg tcacgtgcat
     8041 aagagtacgc gtttacaatt tcggggtttt gaaggatgct gttgtcacgg catgcgcggt
     8101 taattttcac aagatcctga taaacaacac gtccggcacc gatattcagg tgacccactt
     8161 cagaattgcc catttgaccg tcgggcaaac caacattttc tccggaagcc agcaggtagc
     8221 tgttcggata tgtcgctaac aaactatccc aatagggcgt aggagtattg taaatgatgt
     8281 ctgccttgga gtgattacca attccccagc catccaaaat cattaataaa gctttttttc
     8341 tcatatttta taattattac tattctgatt tgcaaatatt tatctgtttt caaaaagcga
     8401 ctgcaaaatt actcaatttt caggatttct atgtcctgtt ttttcatttt tagatgattt
     8461 ttatcatcag ttgtcctatt tattttatct ttgtctaaaa aataaaacaa taaacatgtt
     8521 taaggtcatt gcctgcatga tagcaggaat aggtttaggt tttgtgttgc gcaaccataa
     8581 aatccgtttt atacaaggat ttataataac gcttatctgg ttattattgt tttcgctggg
     8641 gcttgaaata ggctcagata aaaatgtgat tgctcaattc gggaaactcg gatttgaagc
     8701 atttattctg gcatcgatgg ggacgttggg aagtgtgttt tttgctaaac ttctaatgaa
     8761 ttttatacag aaaaaacaaa agaaatagag ttgtattttt ccactacaat ttcagataaa
     8821 aatcctttac caattccgta tattcaggtg tatattgatt tctgatctca gtttcaattg
     8881 ttaaattact tttccgacat gctgtttcag tcatcacaaa ttcgagcaat agccttttgg
     8941 gctgtttaac cgtattggga aaaacattta cttttcttga gcaaaacaaa ccagcttctt
     9001 ttcctgtttt aacacaatcc tcaccttcag caatcggtaa aataatacat aatcttccac
     9061 ctggatttaa caatttcttc gagcagatta ataactgttc gtgtggcaat gtgtcggcat
     9121 gtcttgcaac tgtacgtccc tgtgaaggat ttttaagtga attcctgaaa aatggagggt
     9181 tcgaaatgat tacatcaaat ttttcggagg tgcattctgc aaaatcctgc aacgatatct
     9241 gccgaattat aatcctttct ttccatggag aattctccac atttattgtg gactgaataa
     9301 cactttgatt atcaatatca atggctgtta tttcagcatt gctacgctgt gcaaccatta
     9361 atgcaatcag tcccgagcca caaccaatat ccagcatttt ctttgcattc gtaatatctg
     9421 cccatgcacc aagtgttacc ccatcggcac ccactttcat ggcacattta tcatgaaaga
     9481 ctgtgaattg tttgaaccgg aaataaggat ttgacattgt aaaaattatc ggcgaccgga
     9541 tgatgttcga ctctcggagc gactttctga gcgactttct gatgtacggc ctgaattgct
     9601 gcgaactgag cctgcatccg acgaacgtgt tggagttacc gagcgggttc tggtttgaac
     9661 ttcgcgctgg gttccgtatt gtctggttat tgtcctttcg cggttatcgg ttcttgatgt
     9721 acttcgaaca tcggtgttaa aatccgtatt acccgttccg gttcttccgg ttctctgatc
     9781 actgctattc accctggttt gcggatttgt cctcacatat cggttggcat tgtcaatttc
     9841 aactacctta acttcatttc tccgattttg aagttcatcg tattgttttg aggagagcac
     9901 tttctgaatg tcctcattct tgtttctaat gcgctccata gcctgactac gggtgtttga
     9961 ttgctggcgt tcgcgggcat accgcaaatt tatctggtag atggctttat attgttgttc
    10021 agtcagctgc aattcatttt gcagtttttc tgtttgctta agggcctcct gttcgggagt
    10081 gcgatcagtt actgcagtac tttgtgcaac cactaacaca ggcaaattaa ttactgccca
    10141 acatataaaa atcaaaataa atttcaaacg attcattgta ctattgtaaa tcacattttc
    10201 atcaatgtat ctgcaacaat gattatgcca cattacaaat aattaccaga caagattata
    10261 tttaacctga tttatcaaat aatagctatt tttctgcaat tatttctgaa aatcgcgaag
    10321 cttgatattt gtcagaactt tcttggtatg cagtgtaaaa tcgctgttca taatccatcc
    10381 gtagtatcca atatccgttt tcaagacatc agatactttc tgtcccttat atttaccaaa
    10441 attgataatt tcttcccctt tatcattata aataatccga cccgcaaaat ccacattatt
    10501 ggtttgagcg gtaaatttgg ataaaaactc aacatcattt ttcaaatccg gatacatatc
    10561 aagctgagac ttaagcactt cgtaggtagc ttttgtgtct gcctccgcgc tatgtgcatt
    10621 ttccaggtct ttactacagt aaaatttata agcggctgac agggtgcgct gttccatttt
    10681 atggaaaata acctgaacat caacaaactt acgtttcatc agatcaatat cgacatctgc
    10741 ccgcaacaat tcttctgcca gcaagggtat gtcaaaccga ttggaattat atccggccaa
    10801 atcacatcct tcgatatcgt gaataatctc tttggctact tctttgaata cagggcaatt
    10861 cttcacatct tcatcataaa tgccatgtat ggcagaagaa ctttcgggaa tatgcattcc
    10921 gggatttacc ctgattgttt tagtttcctc cctgccattg ggagagactt tcagatagga
    10981 tatttctatt atccggtcag atacgatatt agttcctgtc gtttcgagat cgaagaaaac
    11041 tattggattt ttcagattga gtttcattat atgatttata ttttacaatt tcaataaata
    11101 aaaagcagaa aaatacgtat tacatatttt tctgcctgta atttgattgt tagttacaat
    11161 tttcagtcat tcagcaacat cggcatcaac agcatgagga tatcctcgtt ttcagcattt
    11221 tccaatggca aaagtatccc tgcgcgcgac ggatcggcta attcaagtat tatatcattg
    11281 gcatcgacat ttcccagcat ttcgaccagg aaagtagatt tgaagccgat ttttatatat
    11341 tctccttcat actgacaggg aatatactct tcggccgaaa tggagaaatc tatatcctga
    11401 gccgaaatat gtatctggtt ctgctgaaac tcgagtttaa tcagattact tgcctgattg
    11461 gagaatactg aaacccgctt caatgcgtta agtaatgtcg tacgatctgc aattacttta
    11521 aacggattat tctttggaat tacgccgttg taatttggat aacgtccttc aacctgccgg
    11581 cataccattg tataattgct cagttgaaag tatgcatttt tttcatcaaa ttttatttcc
    11641 acttcaccca tttctttagg taaaatagtc cgaagcatga tagccggttt tttgggaagt
    11701 ataaagctca atgcttcatc accactgatc gatgcagata aataggcaga tttaaagcga
    11761 actagtttgt gtgcatcggt agcaaccagt gtcagatttt cagacgttat gtcgaaaaat
    11821 ataccattca tcacgggtcg cagctcgtca tcagccatac aaaaaaatgt ctttgatatg
    11881 ccactcaaca aaacaggtac agaaacattt aactgacggg cattttcttc cagtaccgga
    11941 agctgcggat acatatcccc gctttggccg ataaagttgt aagtaccatt tgaagatgta
    12001 atgaccatag ccaggttgct gtcgtcaatc tggaatgcga gtggttgatc tgaaaattcg
    12061 cgtaatgtat cgagcaataa tttgttggca acagcaactt ttccacttcc aaccacatcc
    12121 tgtacctcca tagatgttat cagtgttgtt tcaatatcgg atgcagtcat tgtaagtgta
    12181 ttaccttcaa gacttaaaag aaaattatcc agtatctgta aggaattttt actgttgatt
    12241 acccggctga ttgcttgcaa atgacttaat aattctgtgc ttgatacaac aaatttcata
    12301 tcctttgttt taaatgggtt gttttgatat tacataaata attagcaaat gtactgatta
    12361 tttttaaaaa ataaaacaat agagaagttt tttcgctttt attgggaata taaaaaaact
    12421 aacgcgttat aagattatta tcatttaaat aatgctcttt tgcttttttt tttctacttt
    12481 tgccgcttaa attaattcag tagctaagcc cgatgaaaac aattcaaatt aaagacaaac
    12541 aatttacatt atccatccct tcagaagaaa ttctggaagc agtaaaaaaa gttggagatg
    12601 ccatcaacaa agatctggct gataaaaatc cattatttat ctgtgtacta aacggagcat
    12661 ttatgtttgc gggcgattta atgaaagtgg tggaaatacc gtgtgaaatt acttttatta
    12721 aactttcgtc ctatgaaggt ttaattacaa ccggaatagt caaagaggtt ataggattaa
    12781 atgaaagtgt tgtaggccgg aatgtagttg tagtggaaga tattgttgat acaggtatta
    12841 cgatggataa aatcctgaca tcattgcagg ctaaaggtgc taacgaaata tacgtagcta
    12901 ccttcctcca gaaacctgcc gcacttaaac gggatgtaaa aattgactat gttgccatga
    12961 aaataccgaa tgaatttatt attggatacg ggctggatta tgatggatac ggacgcaatc
    13021 ttaaagacat atacacggtt gttgattgat ttatttcaat tgaccaccaa tttagcattt
    13081 atataacaca ttaatttctc atgatcaatc tcattatctg tggtgcgccc ggatgtggta
    13141 aaggtactca aagtgactta atcgtaagaa aatacaatct tactcatatc tcgacaggcg
    13201 atttactgcg aaaagaaatt gcagacaata ctgaacttgg taaagtagct tatcaatata
    13261 tttcgaaagg tcaacttgta cccgatcaaa tgatcattga tatattggca aataaactga
    13321 attcgcttca gaacagcaat acaaatggca ttattcttga cggctttccc cgtacattag
    13381 ctcaggctga agctctcgaa aaactcttct cagaacgcga tacacaaact gatattctgc
    13441 tcgatcttca ggtcgatgag gacgaactta ttgcccgtct gctgaagcgg ggggaaactt
    13501 ccggacgcag tgatgataat ctggaaacca ttaaaaaacg cctcgatgta tattacaatc
    13561 aaacaaaacc ggtcagtgat tactatcagt cactcggaaa atatgcggga atttatggga
    13621 tgggtacaat agacgaaatt ttcggacgta tttccgagac aattgacgct aaactttaac
    13681 ggtttaacct tgttatattg gtatcaaaat ccattgatga gtttttgaaa ttctgaaatg
    13741 gatttatttt tttcaacaaa ctaacctaaa caacaaacat taatatttcg atctaattct
    13801 aacattatgg caggctccaa tttcatagat tacgtgaaga ttttttgtcg ttccggtaaa
    13861 ggtggtgcag gttcactcca ttttcatcgt gaaaaattta tcgccaaagg tggtcctgat
    13921 ggcggcgacg gaggtcgcgg aggacacatc atactgaaag ggaacagaaa tctctggact
    13981 ttgcttcacc tgaaatatgc acgacatata tatgccggtg atggaggaga tggatcaaaa
    14041 aataaaagtt tcggcaagga tggcaaagat caaaccatcg aagtgccatg tggtactgta
    14101 gtatatgacg gcgaaacggg agaattcctt tgcgacataa ctcatgatgg cgaggaagta
    14161 gtactgatga atggaggacg tggcggcttg ggtaactggc atttccgcac tgcgaccaat
    14221 caggctccgc gctatgctca acccggcgaa ccccgcatag aaaaaacgat tatcatgcag
    14281 ctcaaaattc tggctgatgt tggtctggta ggcttcccca atgccggaaa gtcaacttta
    14341 ctttctgttg tttcggcggc aaaacccgaa attgccgact acccgtttac cacccttgct
    14401 cctaacctgg gaattgtatc ctatcgcgac aataaatcgt tcgtcatggc tgacattccc
    14461 ggaattatcg aaggtgcttc cgaaggtaaa ggtttgggtt tgaggttttt gcgacatatc
    14521 gaacgtaatt caattctgct tttcatggtt cctgccgata cagaagacat aaaaaaagaa
    14581 taccagatat tacttaatga acttcagaaa tacaatccgg aacttatcga caaacagcgt
    14641 atcctggcta tttcgaaatg cgatatgctc gacgaggtgc tcatcgaaca aatgaaaaag
    14701 gagctgcccg atgatgttaa cacacttttt atttcttcag taagcggaac aggaatcacc
    14761 gaactgaaag atgtactctg ggaagaactc aacaaagaca ccaataagat tgtggacatt
    14821 acgcacaaac ctattgaagt tacaggtatg ctcacagaag aagatgaaat ggaaatagag
    14881 gtggatgaat tcgacgatga agatgacttg agcaaataca agggaattgg ttgggatgag
    14941 caataatttt gaatccggtt tcatacaata cccggtatta aacaggtatg acactatccg
    15001 gcattttaca actacccgaa tgggcggagt gagtaccggt aattattcat cattcaatct
    15061 gggactgttt tcaggtgata atcccgatga tttatctaaa aattttaaat tactttccac
    15121 attaaccaat attccggaag acaatatcca ccttccttat cagacacaca gcaacaagat
    15181 ttctctgatt gatgaggatt ttttgagtaa ggataacgaa acccgccatg caatgctaca
    15241 gggtgtagat gccctgatca cgaacatacc caatcaatgt ataggcgttt caacagccga
    15301 ttgtgtaccc gttttgttat atgatcctgt acagcatgtc gttgctgcag ctcatgcggg
    15361 ctggagaggt acctgttcca aaatactcac ccgggtaatt acatccatga aggcagctta
    15421 caatactaat ccccaaaatt taattgcggt tattggtcct tctatttcac ctgaagttta
    15481 tgaagtagga atatctcttt tagaaaaatt cggtgatgcc ggttttaaca tcaggaaaat
    15541 tttcatccat aagaatggca agatttatct cgatttatgg acagcaaata aaacattgct
    15601 tacaaatgcg ggggtgccgg aggaaaatat agagatttcg ggcatctgta cattttcaaa
    15661 tcatgagaag tttttttcag cccggcgatt aggcatatat tcgggaagaa tgatcagtgg
    15721 cattatgtta gtctgaattt attcgaggtt ttcaaggagt ttttcaagtt ccttgtccat
    15781 ttcatgcaac tgctttttac atttttccac cagaaaagca gcctttttca ctttttcctc
    15841 aatcatttcc atattgatct cggtattcat attttcaagt tctgccaaga ttctattcag
    15901 ctcctcaaac gattcggtat aagtttgttt ttttgccata atactacttt gttattcttc
    15961 tgaaaaatga actaaaacac gtctgtatga attgaatatt ttggattttg acacaaatcc
    16021 cagatatctt ttctgctcgt caagcaccgg caggttccac gctcccgaat cttcaaaaat
    16081 ctgcatcact ttttccattg gtaaatttac atcaattgtc gccggaggcg aaatcatcag
    16141 ttgtgcaacc gtaaacctgt tataaagttc gggacgaaac attatatttc tcacttcatc
    16201 cagcaatacc actcctaaca tagtcccttt ctctggttca acgacaggga aaatattccg
    16261 ttttgagcgc gaaatggcag ttacaagctg acctaaagtc atcgaaggtg caacctcagt
    16321 aaaatcagtt tcaatcacat tttccatttt caacagcgta agaacagcac gatctttatt
    16381 gtgtgtgatc aactctcctt tctgtgccag acgcattgca tacaaactgt gtggttcaaa
    16441 tatgattatg gtcagaaaag aaacaaccga tacaatcatc aatgtcatga ataagttgta
    16501 actgccggtt agttctgcta tcagaaaaat tcccgtcagt ggcgcgtgca tgacacctga
    16561 catgagaccc gacattcctg caagcgcaaa gttgggaatt ggtgaaataa ttccaaatct
    16621 gttcataact actgcaacca cgtatccggt aatgcttcct ataaacaagg aaggtgcaaa
    16681 aattccaccc actccaccgg gacttgtggt agcagtggaa gcaaatattt tgaataaaat
    16741 gatcagcacc agatatagta tcaatatcca ttgactgttg ccgaaattca gaaagannnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatca gcaccagata tagtatcaat
    16921 atccattgac tgttgccgaa attcagaaag aagctccggt cgagcaccga agatgcgtta
    16981 ccattcagca gcacctcaat ggtatcatat ccttcgccgt acaagggtgg aaacacaaaa
    17041 atgaggaggc taagtgtgat acctcccagc atcaatttta tcaacggtga ttttacagag
    17101 cgaaaaaagc cttccagaaa gttcattccg cgggtaaaat acaaggacac aaatccacat
    17161 ataacaccta aaagtattag ctgaggtatt cttgtaactg aaaatggttc atattttgtg
    17221 aattcaaaca ggaattcatt ccctgtaaga aagtaagaaa gcgatgtagc tgttattgaa
    17281 gatatcagaa gaggagctat ggatgtcatg gtcatatcga gcatgagtac ttccagggta
    17341 aataccaatc cggctatagg agccttgaaa atgcctccta tcgcacctgc tgcaccacat
    17401 ccaaccatca gaaggagtgt tttctggtct agtttgaaaa attttcccaa attagaacca
    17461 atagctgagc cggttaaaac aataggagct tcggcaccca ccgagccacc aaaaccaatg
    17521 gttacggcac ttccaaccat cgaggtccag gtattatgaa gttttataat cgatttacgt
    17581 tgcgaaatag catataaaat ccgtgtaata ccgtggctga tatcatcctt tactacaaac
    17641 ctgacaaaca aataagcaag aattataccg ataaacggaa aaacaagata ccagtaactt
    17701 accgaattcg gattatacca acttgttaga aggtgctgaa taaaatggat ggatgatttg
    17761 agaataaagg ctgcaagaga ggcaaacaga ccaacaaaaa aactaagaat aagtataaaa
    17821 tgtttatggg caatgtgttc ctcacgccat acaatcattt tattatacca tacttttttt
    17881 tccatttctg ttttttcact aaaaagaaag gcaaagataa tttattttgt ggaatctagc
    17941 ttaacaataa gcggtaaata aaagattcgg ataattacaa actcacttcc tgatgtaata
    18001 acaggtaatc ttatcgcggg
//
LOCUS       DAOPYB010000047        18011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3268_length_18011_cov_9.786589, whole genome shotgun sequence.
ACCESSION   DAOPYB010000047 DAOPYB010000000
VERSION     DAOPYB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18011
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3268_length_18011_cov_9.786589"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PKH58_05390"
     CDS             complement(<1..770)
                     /locus_tag="PKH58_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88493.1"
                     /translation="MMNLNKYPAAAELNLDLTYGSTISINDLQIKTVLIYENGNVLQM
                     NELCRKFLNKSFGGDFNKKKFFLLNNRSEYISILCAARRGGQLANHQMVLHNTDGSST
                     LLTYYLSYAVERDYFVLQFYETSAWQNLYSVSIHNAARNELQKLNPYLNKYGRTLLAD
                     ILQKVNPDNILRLKQYQSYFQNEVLLRISSRYPVLSQTEVSLCGFYALQMSIADMSML
                     LSRSNNSIYVNTHRILRKLGMNSREELITNMCELIKGG"
     gene            1814..5053
                     /locus_tag="PKH58_05395"
     CDS             1814..5053
                     /locus_tag="PKH58_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNX88494.1"
                     /translation="MILKKHLLISLSILAVSIGCAAQPLKNTKPYSIEKDRLLYTVGY
                     AHLDTEWNWNYPTTINELILNTMTENFRLFEKYPGYVFNFTGSRRYNMMKEYYPSLYK
                     KVQHYVQEGRWHVSGSSVDEGEVNISSSESLIRQILYGNRFFFTEFGKESADYMLPDC
                     FGFLSNLPTVFNHCGLLGFSTQKLTWRSAAGVPFNVGVWNGPDEKGVIAALNATDYGG
                     TIEKRLDKDDNWTKRLNEDKTKTGYAFDYRYYGVGDEGGAPREIDVKNVIASQNNSDE
                     NFRVLLTSSDQMYKDITPEIRQKLPIYSGELLLIEHSAGSMTSQSFMKRMNRKNELLA
                     QSSEMAAVYADLWGRSEYPFGKLNSAWDLILGSQFHDILPGTSIPEAYNYAWNDEFIA
                     ANNLAEVLKNSVGSITATMNTKTIGRPIVVYNPVPQPREDIVSAELEYCSVPASVAVY
                     DYTGKQVPAQIAGKNANKLKILFQAKMPPAGIAIFEVRENGKATEPSSLNVTKNSLEN
                     EYYKIRVADNGDIYSIFDKKTGRELLKSPARLEFLKESPAQWAAWNMDWKDRQHAAID
                     YLNQDAALKVIENGPVRVAIEVRRKGLNSEVLQYYSLAAGEAGKRFEVTNKIDWQSRG
                     VSLKASFPLTASNEKATYNLGVSTISRSNNHETQFEVPSKEWFDLTDKSGRYGVSILE
                     DCKFGSDKPDDNTLRLTLLYTPEVNWKWLTYQSTQDWGIHDVKYAVFAHKGNCDEGGS
                     AWQGKFFNQPLLAFEAPKHEGENGKTFSMLSISNPTVGLMAYKKMETGDYYLVRFNEL
                     TGKDQKTVKVKFPADIADAYEVNGQEKKIAAATVKNNELVFDLSHFTIRSFAVRLKTP
                     SRTVETLQTEIVLPYNADFISADTNRWDATLSKSYPAELLPETIISENIHFRMGDKTD
                     EALNAVSCTGQTIQLPNGKYKNLYLLGASLKDKKADFILDGKKITVGFQPWTGTIGQF
                     YNRLLSRDKNTVEQMEEPFVKMDNIAWFASHCHNAYPMKNLAYQYCYLYKYEIPIPEG
                     AKTITLPIDKDIYLFAMTAATTSADAVTPLQALYDNFDGNPSFVLRKKN"
     gene            5253..7055
                     /gene="typA"
                     /locus_tag="PKH58_05400"
     CDS             5253..7055
                     /gene="typA"
                     /locus_tag="PKH58_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNX88495.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKMLVAGHLFRNNEQTGELIMDN
                     NDLERERGITILAKNVSIDYEGYKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKALNLGLKPIVVVNKVDKPNCRPDEVNEMVFDLMYHLNATEDQLDFK
                     TIYGSAKNNWMSDDWRQPSENIRPLLDAIIEYIPEPLMLEGTPQMLITSLDFSSYVGR
                     IAVGRVHRGILKENMDVMLCKRDGSKIKSRIKELHTFTGLGRSKTNEVKSGDICALVG
                     IDGFEIGDTIADIQNPEPLKPIAIDEPTMSMVFTNNDSPFFGKEGKYVTSRHIHERLI
                     KELDKNLALRVEKEINADVWHVFGRGVLHLSVLIETMRREGYELQVGQPQVILKEIDG
                     VRCEPVEQLTINLPEDCSGKIIEMVAIRKGEMISMEAVGDRIQLEFTIPSRGIIGLRN
                     NVLTASAGEAIMSHRFLEYQPYKGEIERRNNGSIISMETGTAFAYALDKLQDRGKFFI
                     YPQDQVYAGQVVGENAKEGDIVVNVTKSKKLTNMRASGADDKTHLAPPVVFSLEEALE
                     YIKEDEYVEITPQSIRLRKIYLDENERKRFLKNN"
     gene            7105..7791
                     /locus_tag="PKH58_05405"
     CDS             7105..7791
                     /locus_tag="PKH58_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX88496.1"
                     /translation="MKTKLILLCIALSTVTFAHAQVGLRGGVNMANEIRTFNKEELQG
                     AFSSDNLTGYQAGLVLQLNPRKSGLGLELGALLSQKGSMFRFDSTGVVNTFKKAYQEI
                     NYIEVPLNLRYRLCLGALGIYGSAGVYGDYALNGKSVFDSQIVTDLETAKNLDDFMDR
                     LDYGYSLGCGIEIMSTIQIGANWSQGLVKKDANKSFTDMIKTSGGVPLPNLKVSSVSK
                     VFSVSLTYLF"
     gene            complement(7848..8450)
                     /locus_tag="PKH58_05410"
     CDS             complement(7848..8450)
                     /locus_tag="PKH58_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HNX88497.1"
                     /translation="MKSSIIILSFFATGLLLGVFRIFPQEWVQSNLSFYILCLLMFSV
                     GISIGQDPNAFKQFRSLSPHMLFLPVMTVLGTYLGVIVVGLFLRDRSLSECLAVGSGF
                     GYYSLSSILITEYKGIELGTIALLVNIFREVLTLLLAPRMVKYFGKLAPISAGGATTM
                     DTTFPVIVKNSGKEFSIISIYHGFIMDFSVPFLVTFFCSF"
     gene            complement(8461..9720)
                     /locus_tag="PKH58_05415"
     CDS             complement(8461..9720)
                     /locus_tag="PKH58_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HNX88498.1"
                     /translation="MFGLIDCNNFYASCERVFNPSLNGKPIVVLSNNDGCVIARSNEA
                     KALGIPMGEPAYKLKELFEKGQVHIFSSNYVLYGDMSQRVMSILASMAPDMEVYSIDE
                     AFLMLDGIPEPEKFGRKLVKTVKQGTGIPVSLGMASTKTLAKVANKFAKKYPAYKNVC
                     IIQTDEQREKALKLLPVGDVWGIGRRITKKLNYYDIKTAWDFTQRSKSWVRSQLGVTG
                     ERTWLELRGTSCLEMEQTATNKTICTSRSFGERLSDINSISEAVCNFAASCGEKLRKQ
                     HSYANSLMVLLQTNPFDTSRPQYYVQEMVQLPAPTNDSAELVHYARLVLERIFREGYH
                     FKKAGVIVMDIVPERPLQTCLFDNRNREKFAKVMETMDKINARYGRQHLRIGSQGFDR
                     KWKLKNEKLSPCYTTSLNSIIDVSAKE"
     gene            complement(9735..10193)
                     /gene="umuD"
                     /locus_tag="PKH58_05420"
     CDS             complement(9735..10193)
                     /gene="umuD"
                     /locus_tag="PKH58_05420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNX88499.1"
                     /translation="MKRNGEKPTLGLQIFSASVESELNLPMVDGGISAGFPSPAQDYI
                     DLKIDLNKELVANPSSTFYGRVRGASMRDAGITDGDILIIDKSIEPKDGDTVVCFIDG
                     EFTLKYIKTEVDAVYLVPANENYKPIKVTEDNNFCIWGVVTYSIHHHHTL"
     gene            10377..10970
                     /locus_tag="PKH58_05425"
     CDS             10377..10970
                     /locus_tag="PKH58_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HNX88500.1"
                     /translation="MKKIVFATNNRHKSEEVKAILAEKAEIFCLADIGCNDDIPETGS
                     TLEENAILKARYIYEKYNIPCFADDTGLEIDALNGRPGVYSARYAGEPSNSAMNMQKV
                     QLEMEGITNREAQFRTVIAYIENGDIQLFEGIVKGAIGVKPEGNGGFGYDPLFIPEGY
                     DMSFASLSPEVKNTISHRAIAMGKFTAYFQNTSEKQS"
     gene            11034..13346
                     /locus_tag="PKH58_05430"
     CDS             11034..13346
                     /locus_tag="PKH58_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX88501.1"
                     /translation="MYKKLTVLLGIIVLGFSLQAQVAMGKWRTHFAYNTVTQIVQSKN
                     KIYAVSDGMLFSIDKRDGGMEFYSKLTGLNGTTISRIGFDQVNNILLIIYQDGNIDFM
                     SASGVKNLPDFYNKQISANKGVNHILFFNNMAYLSCNFGVITLNMKKMEIRDTYYIGP
                     NASEVKVLNTTLHNGSIYAATTTDIFYATATDPNLINYENWQKLNALPGSGEIQGLVS
                     FGSRLVLQRGGKLYIKGNDDIWSDLEKTRDYISVQVIDGHLLALTDVTAYLFNEQLVL
                     AEVNGLSNLRSGLYDAENGEFWFAGDPKGVARCKDNTSAEISYFKPDGPAVNIPYRMR
                     FSGQRLFVVPGGRWSAQFFRAGAVMIYENNIWKNISNNPISAITGKRVLDFMDIAIDP
                     ADNKHFLVSSYGTGVYEFKNDEFVKWYNFKNSLLETVIPDNFEYMRIDGGIFDDKGNV
                     WFTNTSGSKQIKILKPDSTWMGLYYKELEAKYTLGEVLISNQNKNQKWIPGVRAGVGI
                     EIFDDNGTLEDQRDDKSVFYSTFPDTDKGGVISPTNVFSIAQDRNGVVWLGTNEGPLL
                     FNNPSKAFDAGFTCSRVKIPRNNGTNAADYLLIDEQIMAIAIDGANRKWLGTRSSGVY
                     LMSENGQETIRHFTTNNSPLLSNEILSIAINPVTGEVFFGTGNGLVSYQSDAANADDI
                     FNNVHAYPNPVRENYSGIITITGLVADTKVKITDLTGNVVCDTTSNGSIATWDGKNIF
                     GNKVSTGVYLAICVSPDGLHSTTTKILVIN"
     gene            13360..14274
                     /locus_tag="PKH58_05435"
     CDS             13360..14274
                     /locus_tag="PKH58_05435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX88502.1"
                     /translation="MKLSVVISVYNEEQVLPVFIREIAGTLEKDYSEYELIFVNDGST
                     DNSQQLIDEYAGKDNRVKSIRLSRNFGHEAAMIAGIDYATGDAVICMDSDLQHPPSLI
                     REMTERYNNGEFEVINMARNDTKKFSFSNLFYHTLNKLTPYKIEPNASDFFLISKRVA
                     DILRQDYRERVRFLRGIIQIIGFRRTTIEFKSPHRELGKSKYSISKLLSLSVTAIATL
                     SKKPLQAGIYIGLLSGAFSLLLAVYSIIMKIIEKPVSGYTTIVVFLGLMFSIQFFLLG
                     IIGEYVGFLFDEQKARPIYIVDKTINIE"
     gene            14276..14602
                     /locus_tag="PKH58_05440"
     CDS             14276..14602
                     /locus_tag="PKH58_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HNX88503.1"
                     /translation="MGKFLLVGLSILFAVAAQVLIKYSSQEAVGGSKWCYFILLSLFS
                     YASAFVMQSFALKFFPLSRYSASTAIIIMTLVFGCGIFFFNETVQLKQYIGLALGIIS
                     IYLIIS"
     gene            14599..15768
                     /locus_tag="PKH58_05445"
     CDS             14599..15768
                     /locus_tag="PKH58_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88504.1"
                     /translation="MTSIPQKKAFIYAVELLLFLFVNALFILKYVPRTDINAYSVLGI
                     FCIVVLAIVYLFSLPGSKKKEQLSKAGFFLLLSLIIGGIIVFLIKVDRYSVNVDRWSA
                     VTFFLDNLFQGNYPYAAHTHVCETNYPSPFPVWYVVNLPFYLMGDVGLGLIFFILLTA
                     FIVRYYFRSWYKSLFFLLLLSISPAYWWEVMVRSDALSNALLVFTAIIWFNRSGKSLS
                     KNLVIAILICGLIASTRLSAILPLALYFFKPFIQLKLKEKTIFLSGILLIILASFLPF
                     ILWNTETWIFFSRNPFMSQSGVGNTYVLIGMIILGIAFSFRWKGLHRFFNTASLFIFI
                     FMALSQVSLILTRGINGSFFSDSTYDVSYFSLILPYCIGYLSGISLSDDNRTSNI"
     gene            complement(15765..16337)
                     /gene="folE"
                     /locus_tag="PKH58_05450"
     CDS             complement(15765..16337)
                     /gene="folE"
                     /locus_tag="PKH58_05450"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNX88505.1"
                     /translation="MIEFDAEKTKRLAENYREALAVLGENPEREGLLKTPERVAKAMQ
                     FFMQGYEQDPEKILSSAVFTENYRQMVIVKDIDFYSLCEHHLLPFFGKAHIAYIPNKK
                     ITGLSKIARVVEVYARRLQVQERLTTQIKECIQKTLNPLGVMVIIEAQHLCMQMRGVQ
                     KQHSITTTSDFTGVFEQAKTREEFMNLIKS"
     gene            complement(16370..16843)
                     /locus_tag="PKH58_05455"
     CDS             complement(16370..16843)
                     /locus_tag="PKH58_05455"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX88506.1"
                     /translation="MKRFILMNCVLLALMFTVTAQNGSNSATPEIIKSIENGDSKVNV
                     HQDSRLNVLLNDHVNREIKPSGPYSGPGYRVQVFSSNDFKTAKSESASIERQLRSTFP
                     EQNVYRIYASPFWKVRVGDFRSIEEARTFRAELINAFPNLSREAYTVRESKIKIE"
     gene            17001..17684
                     /locus_tag="PKH58_05460"
     CDS             17001..17684
                     /locus_tag="PKH58_05460"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNX88507.1"
                     /translation="MKQQKVAIIVAGGKGERMKASVPKQFIPLGDKPVLMHTLERFYR
                     YSDGIRLILVLPSTQFDVWNELCMTYNFNIPHTVVPGGIARFFSVKNALNIITEDALI
                     AIHDGVRPFIDAGTIQRCFDEAEKSGAAIPVVDLVDSIRQVDWSGSRTVDRKNFRLVQ
                     TPQVFRSDIILKSYQQPYSEQFTDDASVAEAAGYKINLVEGNRENIKITTTFDLKLAE
                     FLLSGDKND"
     gene            17684..>18011
                     /locus_tag="PKH58_05465"
     CDS             17684..>18011
                     /locus_tag="PKH58_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNX88508.1"
                     /translation="MAKRENNIEWLDSLRALAIIGVLMIHISSPVVKMSFGGNMLYWW
                     IGNIVNSTIRFAVPVFLMISGATMLNREYKLGEFYKKRVTRVLVPLLFWMVVYWIFRW
                     FTLSPSI"
BASE COUNT         5305 a         3921 c         3954 g         4831 t
ORIGIN      
        1 tttccacctt ttatcagttc gcacatattg gttatcagtt cttcacggct gttcatacct
       61 agttttcgga gtatgcggtg tgtgttcaca taaatggagt tgttgcttcg gctgagcaac
      121 atcgacatat cggctatgga catttgcaag gcataaaatc cgcacagcga cacttccgtt
      181 tgcgacagca cagggtagcg gctactaatc cgcagtaaga cctcgttttg gaaataggac
      241 tgatactgtt tcaggcgcag gatattgtct gggttaacct tttgcagtat atcagcaagc
      301 aacgtcctac cgtatttatt gagatatgga ttgagtttct gcaattcatt gcgggcggca
      361 ttgtgtatgg aaacggaata aagattttgc caggctgagg tttcgtaaaa ttgaagcaca
      421 aagtaatcgc gttctacagc ataactgaga taataagtaa gcaaggtact ggagccatcg
      481 gtgttgtgca gcaccatctg atgattggcc agttgaccac cccggcgggc agcacagagt
      541 atggaaatat actcactccg attattcagt aagaaaaact tttttttgtt aaaatcaccg
      601 ccaaagcttt tatttagaaa ttttcgacac agttcattca tttgcagcac attaccgttc
      661 tcatagataa gtacggtttt aatctgaagg tcgttaatcg aaatcgtaga tccgtaagtt
      721 aaatccagat taagttctgc agcagccgga tatttgttta aattcatcat attatagatt
      781 atttaatgta aaccaattca acacggcgaa tcattagaaa caagcaaaca agacaaagta
      841 taaaatgcaa tttacgtatt aacaaatatt tagtaagcaa aattcacaaa gcaagtgtag
      901 taaaaataaa accaacaaaa caataattac gtacaataat tgcaaatctg accttatttt
      961 aactcattcg aaccttattt cataaaaaaa catcttacta cacaggactg taaagctaaa
     1021 cacattttaa attcacatta aaattaatgc ttaaaataac acataagaga agaaagaaat
     1081 gtatagtcag acataactga acaccaaaaa aaaaaaaaat ttacatgttc tgttcatttc
     1141 aatattttta ttaaaataaa taaatatccg tttggttagt taaatgattt aatcattcat
     1201 actgtgaaaa aggatttatt aaaaagcaat cgtagtaagg tgtgatgtgt taattataaa
     1261 ataaagaaga cagagctatg ctcctgtgat ttttacgtga ataatattct acaaagtcaa
     1321 cagggataac gtcacttgat cagatatatt tggaacaaca taactgtgat ttatatgtag
     1381 tgtaatggtt gtaaaattgt atttaggggc gtaaccctga ggtcttaatt ctggaagatt
     1441 aggtatccaa acggatactc tctaaaaaaa agaagatctc tcttcctgag cattttgaaa
     1501 atacaagaaa caagatccag tcgcactgca gtaccatctg cgccataaat gcagcttagc
     1561 agaggagaaa aaagcctcat caaaaatcat tcccacaata aataggggga aagattgacc
     1621 aacgggcgtg taagtataaa tgcggataat ttcagtgtcc ttatctgaca atccgccaac
     1681 tttcaggctt ggaatttgtg actatcagct aaacccatac aattatttgc tttctatcag
     1741 ataaaaatgt ttatttttgt aggctataat atcccatcac aagtaaaccg aatatctgaa
     1801 aaaatccatc acaatgattc tgaaaaaaca cctgctaatc tctctgtcaa tcctcgcggt
     1861 atcaatcgga tgcgctgcac agccgctgaa aaacacaaag ccttattcca tcgagaaaga
     1921 ccggctactc tatacggttg gatacgctca tctggacacc gaatggaact ggaactaccc
     1981 cactaccatc aacgaactca ttctcaatac gatgacggag aatttccggt tgttcgaaaa
     2041 ataccccggc tatgttttca atttcacagg ttcgcgacgg tacaacatga tgaaagagta
     2101 ctatcccagc ctctacaaaa aggttcagca ttacgttcag gaaggtcgct ggcatgtttc
     2161 cggctcatcg gtcgatgagg gcgaagtgaa catttcctcc tccgaatcgc tgatacgcca
     2221 gattctgtat ggcaaccgtt ttttcttcac cgaattcggc aaggaaagtg ccgattacat
     2281 gctccccgac tgtttcggtt ttctgagcaa cctgccaacc gttttcaacc actgcggtct
     2341 gcttggtttc tcaacccaga aacttacctg gcgctcagca gcgggcgttc cgttcaatgt
     2401 aggtgtctgg aacggtcccg atgagaaagg agtcatcgcc gctctcaacg cgaccgatta
     2461 cggaggaaca attgaaaaaa gactggataa ggatgacaac tggactaaaa gactaaatga
     2521 agacaagact aaaacgggtt atgctttcga ctaccggtat tatggtgtgg gtgacgaagg
     2581 cggcgcaccc cgtgaaattg acgtgaaaaa tgtaattgcc agccagaata attccgatga
     2641 aaactttcgt gtactgctga cttcgtccga ccagatgtac aaagacatca ctcccgaaat
     2701 acgacagaaa cttccgattt attccggcga attgctgctg atagaacaca gcgcgggttc
     2761 catgacttcg cagtcgttta tgaaacgcat gaaccgtaaa aacgagctgc ttgctcagtc
     2821 gtctgaaatg gcagctgttt atgccgactt atggggcaga tcggaatatc ctttcgggaa
     2881 attgaacagc gcatgggatc tgatactcgg aagccagttt cacgacattt tacccggcac
     2941 cagcattccc gaagcataca actacgcctg gaacgatgaa tttattgccg ccaacaatct
     3001 ggcagaagtt ttgaaaaaca gtgtgggctc aattaccgca acgatgaaca ccaaaaccat
     3061 aggcagacct atagtggttt acaacccggt accacaacca cgagaagata tcgtttctgc
     3121 agaactcgag tactgctcag taccagcatc ggttgccgtg tatgattata caggaaaaca
     3181 agtaccagcc cagattgccg gaaaaaacgc aaacaaactg aaaatcctgt ttcaggcaaa
     3241 aatgcctccg gcaggaatag ccatattcga agtgcgcgaa aatggaaaag caacagagcc
     3301 gtcaagcctg aacgttacaa aaaattcact cgaaaatgag tattataaaa ttcgggtggc
     3361 tgacaatgga gatatttaca gcatatttga caaaaaaacg ggcagggaat tgctgaaaag
     3421 tcctgcacgt cttgaatttc tgaaagaaag tcccgctcaa tgggcagcct ggaatatgga
     3481 ttggaaagac cgtcagcacg ccgccattga ttatctgaat caggatgcag cgttgaaggt
     3541 gatcgaaaac ggcccggtac gagttgccat cgaagtacgt cgcaaaggtc tgaattcgga
     3601 ggttttacag tattatagcc tggcagcagg cgaagccgga aagcgttttg aagtaaccaa
     3661 caaaattgac tggcaatcga gaggcgtcag tctgaaagct tctttcccgc ttaccgcgag
     3721 caacgaaaag gcaacctaca acctgggcgt cagcactatc agccgcagca acaaccacga
     3781 aacacagttc gaagttccgt cgaaagaatg gtttgaccta accgacaaaa gtggcagata
     3841 cggagttagc attctggaag attgcaaatt cggatccgac aaaccggacg ataacaccct
     3901 gcggcttaca ctgctctaca cacccgaagt gaattggaaa tggctcacct atcagagcac
     3961 acaggattgg ggaattcacg atgtaaaata tgcggtgttt gcacataaag gcaattgtga
     4021 cgaaggagga tctgcctggc agggaaaatt cttcaatcaa ccgttgctgg cttttgaagc
     4081 acctaaacat gaaggcgaaa acggtaaaac gttttccatg ctgtccatca gcaatcccac
     4141 tgtcggattg atggcttata aaaagatgga aacgggcgat tattacctgg tacgcttcaa
     4201 tgaattgacg ggtaaggacc agaaaaccgt taaagttaaa ttcccggcag acatagccga
     4261 tgcctacgaa gttaacggtc aggaaaagaa gatagctgct gcaacggtaa aaaacaacga
     4321 actggtgttc gacctcagtc actttaccat ccggagtttc gccgtcagac taaaaacccc
     4381 tagtcgtacc gtggaaacgc tgcaaaccga aatcgtactg ccttataacg ccgattttat
     4441 aagcgctgac accaatcgct gggatgctac cctttcgaaa tcttatccgg ctgaattact
     4501 tcccgaaacg ataatcagtg agaatataca tttcagaatg ggcgacaaaa ccgacgaagc
     4561 cctgaatgcg gttagctgta ccggccaaac catccagcta cctaacggta aatacaagaa
     4621 cctatacctg ctgggagcct ccctgaaaga taaaaaagca gatttcatac tcgacggtaa
     4681 aaaaataact gtaggttttc aaccctggac aggaactatc ggacaatttt acaaccgcct
     4741 gttgagccgc gataaaaaca ctgttgaaca aatggaggaa ccattcgtaa aaatggataa
     4801 tattgcctgg tttgcatcgc attgtcataa cgcctaccct atgaaaaatc ttgcctatca
     4861 gtactgttat ctgtataaat acgaaattcc aattccggag ggagctaaga caatcacgct
     4921 tccgatcgat aaggacattt acctgtttgc aatgacagcg gcgacaacat cggcagatgc
     4981 agtcacaccg cttcaggctt tgtacgataa tttcgacggt aatccatctt ttgtactccg
     5041 aaaaaagaat tgaaaaatcc acttcttacg aaagaaatgt ggtatcaaca aataattcca
     5101 catttctttc gttgcagatt ttaagaatgc tatcagccat ttatccattc acatttatta
     5161 tttcctgata tttaacgcat agtaatacag acatatcggt taaatttcgt agattttcac
     5221 tacctttgca ccctcaaata aaaaaggtat aaatgcaaaa gattagaaat attgccatta
     5281 tcgctcacgt tgaccatggc aaaacaacat tagttgataa aatgctggtt gccgggcatc
     5341 tgtttcgcaa caacgaacaa accggtgaac ttattatgga caataacgac ctggaacgcg
     5401 aacgtggcat caccattttg gccaaaaacg tttcgatcga ttatgaagga tataaaataa
     5461 atattattga cactccgggt cacgccgact ttggcggaga agtggagcgc gttctcaata
     5521 tggccgacgg cgtgctgctt ttggtcgacg ctttcgaagg tcccatgccc caaacccgtt
     5581 ttgtgctgca aaaagcgctt aacctgggat tgaaacccat cgtagtggtc aataaagtgg
     5641 ataaacccaa ttgccgaccg gacgaagtca acgaaatggt attcgacctg atgtaccacc
     5701 tcaatgccac agaagatcag ctggacttca aaactatcta tggttcggct aaaaataact
     5761 ggatgtcgga cgactggcgc cagccctcag aaaatatccg tccgctgctc gacgccatca
     5821 ttgaatacat tccggaaccg ctaatgctgg agggaactcc gcagatgctc atcacttctc
     5881 tcgacttctc gtcgtacgtg ggacgtatcg ccgtagggag agtacaccgt ggcattctga
     5941 aagaaaacat ggatgtaatg ctctgcaaga gagacggttc aaagattaaa tccagaataa
     6001 aggaactgca taccttcacc ggtctgggac gttcgaaaac aaacgaagta aaatcgggag
     6061 acatttgtgc actggtaggt atcgacggtt ttgaaatcgg tgataccatt gccgatattc
     6121 aaaatcccga acctctgaag ccaattgcca ttgatgagcc aacgatgagt atggttttca
     6181 ccaataacga ctcacccttc tttggcaaag agggaaaata tgtaacttcg cgccacatcc
     6241 acgaacgttt gattaaggaa ctggacaaaa acctggccct gcgtgtggaa aaagagatca
     6301 atgccgatgt ctggcatgta ttcggacgcg gggtgcttca cctgtcggta cttatcgaaa
     6361 ccatgcgccg cgaaggttac gaattgcagg tgggtcagcc acaggttatc ctaaaggaaa
     6421 ttgacggtgt acgatgcgaa ccggtagaac aactaaccat caacctgccc gaagattgct
     6481 ctggaaaaat tatagagatg gtggcaatcc gcaagggaga aatgataagt atggaagctg
     6541 ttggcgaccg cattcagctg gaatttacca taccttcccg cggtattatc gggctgcgca
     6601 acaatgtgct gaccgcatca gcaggagaag ccatcatgtc gcaccgtttt ctggaatacc
     6661 agccatataa aggtgaaatt gagcggagaa ataacggttc aatcatctcc atggaaacag
     6721 gtaccgcctt tgcttacgcg ctcgacaaac tccaggatcg cggaaaattc ttcatctatc
     6781 cgcaggatca ggtatatgcc ggtcaggttg taggcgaaaa tgcaaaagaa ggcgatatcg
     6841 tggtaaatgt taccaagtcg aaaaagctga caaacatgcg cgcttccgga gccgatgaca
     6901 agacacatct ggcacctccc gtcgttttca gtcttgaaga agcgttggaa tacatcaaag
     6961 aggacgaata tgtggaaatt acaccacaga gcatccggtt gagaaagatc tatctggatg
     7021 aaaatgaaag aaaacgtttc ttaaaaaata attaatcaaa ctattcgtta tatttgcaga
     7081 ataataaaag tcagatatcc ggatatgaaa acaaaactta tcttattatg catcgctctc
     7141 tcgaccgtga cattcgcaca tgcgcaggtg ggattacgcg gcggagtgaa tatggccaat
     7201 gaaatccgca cttttaacaa agaggaactt cagggcgctt ttagcagtga taatcttacc
     7261 ggttatcagg cagggctggt ccttcagctc aatcccagaa aatcggggct tggtctggaa
     7321 ctgggagcgc tgctgtcaca gaaaggcagc atgtttcgct ttgacagtac cggggtggtt
     7381 aatactttca aaaaagcata tcaggaaata aactatattg aagtaccgct gaacctccgg
     7441 tacagactct gtctgggagc gcttggcatt tatggttctg ccggagttta cggcgattat
     7501 gcgctgaacg gcaagtcggt cttcgactct caaatcgtca ccgatttaga aacagccaaa
     7561 aacctggatg attttatgga cagacttgac tatggttaca gtctgggttg cggtattgag
     7621 ataatgagca ccattcagat tggtgctaac tggagtcagg gattggtaaa gaaggatgcc
     7681 aataaaagtt ttaccgatat gattaaaaca tcgggcggtg tgccactccc caacctgaag
     7741 gtttcttccg tttcgaaagt cttttcggta tcgctgacgt acctgttctg aaacacgaat
     7801 tacaagttga taatatataa aaagagcggt ttcgctcttt tttcgtttta aaaactgcaa
     7861 aagaaggtaa ccagaaacgg cactgagaag tccatgataa aaccatgata aatggatatg
     7921 attgagaact cttttccgga atttttcaca attaccggaa aggttgtatc catggtagta
     7981 gctcctccgg ctgagatggg agccagtttt ccaaaatact tcaccatccg gggagccagt
     8041 agcagggtga gcacttcgcg gaaaatattc accagcaaag cgatggtacc cagttcgata
     8101 cccttgtatt cggttatcag aatgctcgaa agcgaatagt aaccaaaccc ggagccaacc
     8161 gccagacatt cggaaagcga acggtcgcgc agaaacaatc ccactacaat gacacccaga
     8221 taagtaccca acacggtcat taccggtaaa aaaagcatgt ggggagaaag gcttctgaat
     8281 tgtttaaacg cgtttgggtc ctgaccgata cttatcccca cgctgaacat gagaagacaa
     8341 aggatgtaga aactcagatt ggactgtacc cattcctgcg gaaaaatccg aaaaacgccc
     8401 agcaaaagtc ctgtcgcgaa aaaagataat attataatgc tgcttttcat acgtttattg
     8461 ttattctttg gccgaaacgt cgatgatact gttcaacgag gtggtgtaac agggtgacag
     8521 tttttcattt ttcagtttcc atttacggtc gaaaccctgt gaaccaatcc tgagatgctg
     8581 acggccgtat cgcgcgttga ttttatccat cgtttccatt actttcgcaa atttttcgcg
     8641 attgcgattg tcgaacaggc aagtctggag cggtctttca ggcacgatat ccatgactat
     8701 aacacctgcc tttttaaagt gatatccctc cctgaaaatc cgctcaagca ccagccgcgc
     8761 atagtgaacc agttcggcgg aatcgttggt gggagccggg agctgcacca tttcctggac
     8821 gtaatactga ggccgggatg tatcgaaggg attggtctgc agaagcacca tcaacgaatt
     8881 ggcatacgaa tgttgctttc tcagcttctc gccgcacgat gcggcaaaat tgcacacagc
     8941 ttccgaaatt gagttgatat ccgacaggcg ttcaccaaaa ctccgggaag tgcagatggt
     9001 tttattggta gccgtttgtt ccatttcaag acacgatgtg ccacgcaatt ccagccaggt
     9061 tcgttcaccc gtcacaccaa gctgactgcg cacccaggat ttgcttcgct gtgtgaaatc
     9121 ccaggcagtc ttaatatcgt aatagttcag tttctttgta attcgtcgtc cgatgcccca
     9181 cacatctccc actggaagca gtttcagggc tttttcgcgc tgctcgtccg tttgaatgat
     9241 gcagacattc ttgtaagccg gatacttctt ggcaaactta ttggccactt tagccagtgt
     9301 tttggtggat gccatgccca gacttaccgg aatgccagtg ccctgtttca cggttttaac
     9361 cagtttacgt ccgaattttt ccggctcagg gataccatcg agcatcagga atgcttcatc
     9421 gatggaatac acttccatgt cgggcgccat cgaagccaga atgctcatca cccgttgcga
     9481 catatcgccg tagagtacat aatttgatga aaaaatatga acctgtcctt tttcgaacaa
     9541 ttccttaagc ttgtaagccg gttcacccat cgggattccg agcgctttgg cttcgttgga
     9601 acgtgcgata acacatccgt cgttgttgga cagcacgacg ataggttttc cgttgagcga
     9661 aggattgaaa acccgttcgc aggacgcgta gaagttattg cagtcaatta agccaaacat
     9721 gatttcaaat gcagttacag cgtatggtga tgatgaatgg agtatgttac caccccccag
     9781 atgcagaaat tattatcttc cgtcactttt atcggtttgt agttttcgtt ggcgggaacc
     9841 agataaaccg cgtccacttc ggttttaata tatttcaggg taaattcacc gtcaataaaa
     9901 cataccacgg tatcgccatc tttcggttcg atagacttgt cgatgatgag aatatcgccg
     9961 tcggtaatgc ccgcgtcgcg catggaagct cccctcacgc gtccgtaaaa agtggatgaa
    10021 ggattggcta ccagttcctt gttcaggtca atcttcagat cgatataatc ctgagccgga
    10081 ctggggaaac cggcagatat gccgccatct accatgggca ggtttagttc cgattctaca
    10141 gacgcgctaa atatctgcaa accaagcgtt ggtttttcac catttctttt cattgaagtt
    10201 cattcttttt cttacaaaag taagcatttt ttgccggaag aaaaaagaga cattatttct
    10261 ttagatataa acaaatccgg gcagaatgtg attacaacct tcaggaaaat tttacatatt
    10321 tttctgtatc tttgtaaaag taattatctt tgttacttta acgaaaatgt tccgttatga
    10381 aaaaaatcgt attcgccacc aacaaccgtc acaaatcgga ggaagtaaaa gcaattctgg
    10441 ctgaaaaagc agagattttc tgtctggctg atatcggttg caatgacgat attcctgaaa
    10501 caggcagtac gctcgaagaa aatgccatac tgaaagcacg ttatatatac gaaaaataca
    10561 atattccctg ctttgcagat gataccggac tggaaattga cgcgctgaac ggcaggccgg
    10621 gagtttactc agcacgttat gcgggcgaac cttccaactc tgccatgaat atgcagaaag
    10681 tgcagctcga aatggaaggt atcacaaacc gtgaagctca gttcagaacg gtgattgcat
    10741 atatcgaaaa cggcgacatt caactcttcg aaggaattgt aaaaggcgct atcggtgtga
    10801 aacccgaagg aaacggtggc ttcggatacg atcctctgtt tattcccgag gggtacgaca
    10861 tgagttttgc ctcactaagc cctgaagtta aaaatacaat cagccatcgt gcaatagcca
    10921 tggggaaatt tacggcatat tttcagaaca cttcggagaa acagtcataa ctgcttcaca
    10981 ataattcagt taaaacattg ttttttcttt atcgaaaaat aaaaaaatca tacatgtaca
    11041 aaaagttaac agttctcctg ggtataatcg ttctcggatt cagtttgcag gcacaggttg
    11101 ctatgggcaa atggcgtacg cattttgcat acaacactgt tacacagatc gttcaatcga
    11161 aaaataagat atatgccgta agcgacggaa tgcttttctc catcgataaa agagatggcg
    11221 gtatggaatt ttacagcaaa ctaacgggac ttaacggcac caccatctca agaatcggat
    11281 tcgatcaggt caacaatatc ctgctgatta tctatcagga tggaaatatc gatttcatgt
    11341 cggcttccgg agtgaaaaac cttccggatt tctacaacaa gcaaatcagc gccaacaagg
    11401 gcgtaaacca tattttgttt tttaacaata tggcatacct ctcttgcaat ttcggggtca
    11461 ttaccctgaa catgaagaag atggagatcc gcgacaccta ctacatcgga ccgaatgctt
    11521 cggaagtaaa agttctcaac acaaccttac acaacgggag catttacgcg gctacgacca
    11581 ccgatatttt ctatgcaacc gccaccgatc cgaatctgat taattacgaa aactggcaaa
    11641 aactgaacgc cctacccggt tccggtgaaa ttcagggact ggtatccttt ggcagccggc
    11701 tcgtcctgca aaggggagga aagctatata taaaagggaa tgacgatatc tggtcggacc
    11761 ttgaaaaaac gagggattat atctctgttc aggttatcga cggacatctg cttgcattaa
    11821 ccgatgtaac agcctatctg tttaacgaac agctggttct ggcagaggta aacggtctga
    11881 gcaatctgcg cagcggattg tatgatgctg aaaacggcga attctggttt gcgggtgacc
    11941 caaaaggagt ggcacgttgt aaagacaaca ccagcgctga aataagttac ttcaagccgg
    12001 atggtccggc cgtgaatata ccttaccgga tgcggttctc gggacaaaga ctttttgtag
    12061 ttccgggagg gcgttggtca gctcagttct tccgcgcggg tgctgtaatg atctatgaaa
    12121 acaacatctg gaaaaacatc agcaataatc ccataagcgc aataacaggc aaacgtgtcc
    12181 tggatttcat ggatattgcc attgatccgg ctgacaataa acactttctg gtaagttcat
    12241 acggcaccgg agtttatgaa tttaagaacg atgaatttgt gaaatggtat aatttcaaaa
    12301 acagtctgct cgaaaccgta attccggaca attttgaata tatgcgtatt gacgggggaa
    12361 ttttcgatga caaagggaat gtttggttta ctaatacatc cgggtccaaa caaataaaaa
    12421 tactgaaacc cgatagtacc tggatgggac tttactataa agagctggag gcaaaataca
    12481 cattgggtga ggtacttata tcaaatcaga acaaaaacca aaagtggata ccgggagtta
    12541 gagccggagt tggaattgag atatttgacg acaacggcac gctcgaagat cagagagatg
    12601 ataagtcggt attttattcc acatttccgg atacagataa gggaggtgta atctctccga
    12661 ccaatgtttt ttccattgca caggacagaa acggagttgt ctggttgggc accaacgaag
    12721 gtccactgct tttcaacaat ccttcgaaag catttgatgc cggctttacc tgttcacggg
    12781 tgaaaatacc acgcaataac ggtacaaatg cagccgacta cctgttgatt gatgaacaaa
    12841 taatggcaat tgccatcgac ggagctaacc gaaaatggct cggaacaaga agttcgggtg
    12901 tttatctgat gtcggaaaac ggacaggaaa ccattcgtca cttcacgacc aacaacagcc
    12961 cgctgctatc aaatgagata ttatccatag ccatcaatcc ggtgaccggt gaagtttttt
    13021 tcggcacagg caacggactg gtatcctatc agagcgatgc tgcaaatgcc gacgatatat
    13081 tcaacaatgt tcacgcttac ccaaatcctg ttcgcgaaaa ttactcaggc atcatcacca
    13141 tcaccggtct ggtagccgat accaaagtaa aaatcacaga tctgacaggg aatgtggttt
    13201 gtgatactac ctcgaatgga agcatcgcaa cctgggatgg caaaaacatt ttcgggaata
    13261 aagtaagcac cggagtttat ctggccatct gtgtatcgcc cgacggactg cacagcacaa
    13321 ccaccaaaat actcgtgata aactaagatt aaaagcgcca tgaagctatc cgtcgttata
    13381 tccgtctaca atgaagaaca ggtactgcct gttttcatcc gggagatagc cggtacactt
    13441 gaaaaggatt attcagaata tgaattaatt ttcgtcaacg atggcagtac ggacaactcc
    13501 cagcagctga ttgatgaata tgcaggaaaa gataaccggg tgaaatcaat ccgactatcg
    13561 agaaatttcg gacatgaagc agccatgatt gcaggcattg attatgctac cggcgatgct
    13621 gttatctgca tggacagtga cctgcagcat ccgccctcgt tgattcggga aatgaccgaa
    13681 cgatacaaca acggagaatt tgaagtgatc aatatggcca ggaatgatac gaaaaaattc
    13741 agcttttcca acctgtttta tcatacactt aacaaattaa caccctataa aattgaaccc
    13801 aatgcgtcgg attttttcct gatttcaaaa cgtgtggccg acatactccg tcaggattac
    13861 cgtgaacggg ttcggttcct gcgcggaata attcagatta tcggtttcag aagaacaacc
    13921 attgaattca aatcgccaca cagggaactg ggtaaaagca aatattccat ctccaaactg
    13981 ctttcgttat cggttacagc tattgccacc ctgtcgaaaa aaccattaca agctggaata
    14041 tacatcggac ttttatcagg tgcgttcagt ctgttacttg ccgtttacag cattatcatg
    14101 aaaatcattg aaaaaccggt ttcgggatac accaccatcg tggtttttct cggcctgatg
    14161 ttcagcatcc agttttttct gctcggcatc atcggtgaat atgtcggttt tttgtttgat
    14221 gaacaaaagg caagacccat ttacattgtt gacaaaacga taaacatcga ataaaatggg
    14281 aaaatttctg cttgtaggat taagcattct gtttgccgtg gcagcacagg ttctgatcaa
    14341 atattcctcg caggaagcag ttggcgggtc gaaatggtgt tatttcatct tactgagttt
    14401 attctcatac gcgtcggcat ttgtcatgca gtcgtttgcc ctgaagtttt ttccattgag
    14461 ccgctattcg gcaagcactg ccataataat tatgacactt gttttcggat gcggcatatt
    14521 ttttttcaac gaaacagtgc aattaaaaca atatattgga ttggcgctgg gtatcatttc
    14581 catctacctg atcatatcat gacatctatc cctcaaaaaa aggcattcat ttatgcggtt
    14641 gaactgttgc tttttctttt tgtcaacgcg ctgtttatcc tgaaatatgt accccgaacc
    14701 gacatcaatg cttactcggt attgggtatt ttctgtatcg ttgtacttgc aatcgtgtat
    14761 ctgttttcgc tacctggaag taaaaagaag gagcagttgt caaaggcagg atttttcctt
    14821 ctcttgtccc tgattattgg agggattatc gtttttctga taaaggttga ccggtacagc
    14881 gttaatgttg acaggtggtc ggcagttact ttttttcttg acaatctgtt tcagggaaac
    14941 tacccctatg cggcacatac acacgtatgc gagacgaatt acccctcccc ttttccggtt
    15001 tggtatgtcg taaatttacc gttttatctg atgggagatg ttggtcttgg gctgatattt
    15061 tttattctgc tgaccgcttt tatcgtccgt tattatttcc gctcgtggta taaaagtttg
    15121 tttttcctcc tgcttctcag catatcgcct gcttactggt gggaagtaat ggttcggagc
    15181 gatgcgttga gcaacgcgct gcttgtgttc acagctatta tatggttcaa ccggtcaggg
    15241 aaaagccttt cgaaaaattt agtcattgct attctgatct gtgggcttat tgcttccaca
    15301 cgtttatcgg ccatactgcc gctggcactg tatttcttca aacccttcat ccaactaaag
    15361 ttgaaagaaa agacaatatt cttatcgggt atattgctga taattttggc aagttttcta
    15421 ccctttattc tctggaatac tgaaacatgg atattcttca gccgcaatcc gtttatgagt
    15481 cagtcgggag tgggcaacac atacgtgctt attgggatga taattcttgg aatagcgttt
    15541 tcgttcaggt ggaaagggct tcacagattt ttcaataccg catcgctgtt tattttcatc
    15601 ttcatggcat tgtcgcaagt ctctctgatc cttacccgcg gcatcaacgg aagttttttt
    15661 tcagattcca cctatgatgt ttcttatttc agtttgatat tgccttactg cattggttat
    15721 ctgtcgggca taagcctttc ggacgataac aggacatcaa acatttagct ctttatcaga
    15781 ttcatgaatt cctcgcgggt ttttgcctgt tcgaatacac ctgtaaaatc tgaagtcgtg
    15841 gtaatggagt gctgtttctg aacaccgcgc atttgcatgc aaagatgctg tgcctcaata
    15901 ataaccatta cacccaatgg attcagtgtt ttctgaatac attccttgat ctgggtagta
    15961 agccgttcct gtacctgcag tctgcgggcg tacacttcca ctacacgggc tattttgctg
    16021 agaccggtaa tctttttgtt gggtatgtag gcgatatgtg ctttgccgaa gaaaggcagt
    16081 aaatgatgtt cgcaaagaga gtagaaatcg atgtccttaa caatgaccat ctgacggtaa
    16141 ttctccgtga agacagccga gctgagtatt ttttcgggat cctgttcata gccctgcata
    16201 aagaactgca tggctttggc aacccgttcg ggagttttca gcaggccttc gcgttcggga
    16261 ttttcaccca gaacagcaag ggcttcacgg taattctcgg ccagacgttt agtcttttcc
    16321 gcatcaaatt ctatcatgtt attggcgtac aaatccattg gagtttcttt tattcaattt
    16381 ttattttgct ttcgcgtacc gtgtatgctt ccctgctgag attgggaaat gcattgataa
    16441 gttcagcccg aaatgtgcgc gcttcctcaa ttgatctgaa atcacccact ctgactttcc
    16501 agaaagggga tgcataaatc cggtaaacat tctgttcggg gaatgtactg cgcagctgac
    16561 gttcgatgga tgcggactcg gattttgccg ttttgaaatc gttgctcgaa aaaacctgaa
    16621 cacgatatcc ggggcctgag tatggtcccg agggtttaat ttcacgatta acatgatcat
    16681 tcaggagtac gttcagacgg ctgtcctgat gaacattcac tttcgagtct ccgttttcaa
    16741 tcgatttgat gatttcgggg gttgctgagt tggatccgtt ttgtgctgtc actgtaaaca
    16801 tcaacgcgag cagaacacag ttcattaaaa taaatctctt catttaggta agctgaaaat
    16861 ttgcacaaag gtatgaaatt gatacgattt acaagttttt tagcaaataa aaacagtcct
    16921 gcctgttcag tttccatttt atttcattgt tatttggtcc gaaaaagact atctttgttg
    16981 ctcttactac ttttttattc atgaaacagc aaaaagtggc aattattgta gccggaggca
    17041 aaggcgagcg gatgaaagca tctgttccta aacaatttat ccccctgggg gataaaccgg
    17101 tactgatgca caccctggaa agattttacc gatattcaga tggcatcagg ctcattctgg
    17161 tgctaccttc cactcagttt gatgtatgga atgaactctg tatgacttat aacttcaaca
    17221 taccccatac agtcgttccg ggaggaatcg cccgcttctt ttctgtaaaa aacgcgctca
    17281 atatcatcac tgaagatgct ctgattgcca tccacgacgg tgttcgtccg tttattgacg
    17341 ccggtactat acaacgctgc ttcgacgaag ctgaaaaatc cggcgctgct attccggttg
    17401 tcgatttggt ggacagcata cgccaggtgg actggagcgg cagcagaact gtggaccgca
    17461 agaatttccg gttggtgcaa acacctcagg ttttccgctc agacatcatt ctgaaaagtt
    17521 atcagcagcc ctacagcgaa caattcaccg atgatgcatc cgtggcagaa gcagccggat
    17581 ataaaataaa cctggtggaa ggaaaccgcg aaaacataaa aatcaccacc acctttgacc
    17641 tgaaacttgc agaatttctc ctttcgggag ataaaaatga ctaatggcta aacgtgaaaa
    17701 caatatagag tggctcgaca gcctgcgtgc actggcaatt atcggtgtgc taatgattca
    17761 catatcttca ccggtggtaa aaatgtcgtt tggaggcaat atgctttact ggtggattgg
    17821 taacatagtg aacagcacca tccgttttgc tgtaccggtt tttttgatga taagcggtgc
    17881 aactatgctc aaccgggaat ataaactggg cgaattttac aaaaaacgcg tgacacgtgt
    17941 gcttgttccg ctcttgttct ggatggtggt ttactggatt ttcaggtggt ttacgctttc
    18001 tccgtccatt c
//
LOCUS       DAOPYB010000048        17603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3381_length_17603_cov_9.139788, whole genome shotgun sequence.
ACCESSION   DAOPYB010000048 DAOPYB010000000
VERSION     DAOPYB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17603
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3381_length_17603_cov_9.139788"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..2505
                     /locus_tag="PKH58_05470"
     CDS             310..2505
                     /locus_tag="PKH58_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNX88509.1"
                     /translation="MFALISIGWLGYLPEIKELQNPINKAATEIYSSDMALLGRFSLA
                     KENRVPINYNEIDKDVINALIATEDVRFYKHSGVDGRALIRVFFGLFTNSNSGGGSTI
                     TQQLAKQLYSPSASNIFKRALQKPIEWVIAVKLERMYSKEEIIAMYLNQFDFLNNAVG
                     IKSAAQVYFNTTADNLSIEQAATLVGMCKNPSYFNPVRHIERTLGRRNVVFDQMRKAD
                     MITREQRDSLSKLPLKLNYKRVDHKEGLATYYREFLRQILMADEPSRSDYADWQKQKY
                     LDDKWAWENNPLYGFCKKNKKPDGSNYNIYTDGLKIYTTIDSRMQRYAEEAVEEHMLK
                     LQKNFFREKRGRSYAPFSRDLSPQEIDDILKRAMRQSERYRLMSDAGMTKAEITKAFN
                     TKTDMQVFSYNGMIDTTMTPMDSIRYHKHFLRCGFMSMDPSNGYVKAYVGGTNITAFQ
                     YDMVSSGRRQIGSTVKPYLYTLAMEEGMSPCDRVLCAPITIQTSSGPWTPKNSSSARM
                     GEMVTLRWGLANSNNWISARLMNQYTPETLVKLMQSFGIRGHLDPVVSLALGPCEVSV
                     SEMVDAYTAFPSKGIRVEPLYVTRIEDANGNVIGSFVPKMQEIFSETTAYKMIYLLRS
                     VVDEGTGGRIRSVYGLTMPVGGKTGTTQNNSDGWFMGFTPKLVSGVWVGGEERSIHFD
                     GMAQGQGASMALPIWAIYMKKVYANPELGYRYNDQFDVPADFNPNEACE"
     gene            2578..4599
                     /locus_tag="PKH58_05475"
     CDS             2578..4599
                     /locus_tag="PKH58_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX88510.1"
                     /translation="MKKLIYSVLFLAIAQLSFSQWNTDRIMNIGKNALYYEDFVLSIQ
                     YFNQVIKIKPYLAEPYMYRGIAKISLGDYTGAEQDCSSAIERNPFVPQAYFARGYARN
                     NINKYEEAISDFNKALEFSPENTDVIANRGEAKERINDFEGAINDWNLYRKLNPKSRG
                     IDYEIGRIQLAMKDTVAAMASLDKHILSDSTNAMGYSIRALIKMLKKDENGAYKDYSK
                     AVAYKSTYPGDYINRGILNVQRNKFNLALSDYNEAIRLDPKNILAYYNRGLLRANLGD
                     SNNAIGDLEKVVAADTTNYEARLQKAYMELKVGDMTAAVNDFNFILKKYPFFVPAYYG
                     IADAKSATGRKKEAGQYRSLASEIINNKDFYKRKQNLVAKNQIAKDTPVNDMKKSVNL
                     LEKLIVRDTDDQKYENKYGEGVRGEIQNSYADLKNERNFELNYFARVQETRRTNTYHP
                     LISRFNGENMLSSSLKITNDVVTFTADLINSRFDEINKLTERIKNNPEDANLYFLRAL
                     EFSHVQDYSSAIDDLNHAITLHTDFALAYFTRANLRCKMIEYQNYNQNDEKSDEKNKT
                     NIAVKEESNKLNYDLIIRDYDKTTDLAPDFSFAYYNKANILSSLKNYKSAIIAYSKAI
                     EIDPDFAEAYFNRGIAYLYMNDQANAILDLSKSGELGIFQSYSIIKRIK"
     gene            4812..5792
                     /locus_tag="PKH58_05480"
     CDS             4812..5792
                     /locus_tag="PKH58_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNX88511.1"
                     /translation="MPFVFNNKSTKVQQLADYLTQLITVREYKVGEKLPSINELSRKH
                     NVSRDTVFKAFLELKERGVIDSLQGKSYFVASQNSNILLVLDEYTSFKDVLYNSLIKR
                     LPENTKIDLWFHQYNENLFNTIVNSSVGRYNQYLIMNYKNDEYSQVLEKIDRSKLLLL
                     DFGKFDKDGRSYICQNFDESFYEALTTIKDNLKKYKRLLFVINKLHRHPRSSVEWFMK
                     FCNDNGFFGEICEEISDQTIEKDDFYIIVKQEDVVKMIKKSRVAELKLGTDVGLLAYN
                     DMPFYEIIENGISSISIDWEEMGNYAAEFIVKKEPIQRFLKTKIIKRNSF"
     gene            complement(6075..6203)
                     /locus_tag="PKH58_05485"
     CDS             complement(6075..6203)
                     /locus_tag="PKH58_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88512.1"
                     /translation="MIINTVNAINVERYIAIFLSIFVDVKLSKDNDSVDIPSIYNT"
     gene            6505..7362
                     /locus_tag="PKH58_05490"
     CDS             6505..7362
                     /locus_tag="PKH58_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX88513.1"
                     /translation="MVTVLSYGVVVTRDDFYLKKQIIYVDDLPVNFDNYRIAMFSDLH
                     LGNWDNKYMIMKPIIKMINSGNPDIIVFSGDMINNYENETLGWEPYFRQLKARQGIYA
                     VLGNHDYGDYTKWKSEKAKAENLEKTKSNIRNLGFRLLLNESVELVKGSDTITLAGVE
                     NYGSGHFENHGDLAAALKGTKPTRKKILITHDPNHWDDEVVSKRKDIFLSLSGHTHAG
                     QIGYSNGNIQFSPSSVVYPHWDGLYSKGSQYLYVNRGAGFVGIPLRIGVPPEITLIVL
                     KRKHKRIES"
     gene            complement(7365..8033)
                     /locus_tag="PKH58_05495"
     CDS             complement(7365..8033)
                     /locus_tag="PKH58_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNX88514.1"
                     /translation="MSILQNLTEIRRNIPGNVQLVCVSKFHPESAIREAYACGERIFG
                     ESRVQELTAKQAQLMSDIHWHFIGHLQSNKIKYIVPFVSLIHGVDSFKLLKEINNQAY
                     KAGRVVPCLLQIHIAQEETKFGFSVEEIHEMLNNFEFPTFKNIEIRGLMGMATLTENK
                     SQIRDEFRSLKHLFEELKKDYFTGDDCFSELSMGMSDDYKIAIEEGSTMVRIGSLIFG
                     TREY"
     gene            complement(8076..8507)
                     /locus_tag="PKH58_05500"
     CDS             complement(8076..8507)
                     /locus_tag="PKH58_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HNX88515.1"
                     /translation="MHNWFQCKIKYEKNAEDGGVIKVSEAYLVDALSFTEAEERINEE
                     MRPFISGEFLVSDIKRAKINELFTNENGDRWYRAKVNFISLDEEKGIEKRIATTMFAQ
                     ASTLKDAFEVIEKGMKDTLADYDIAAIAETTILDVFPYKAE"
     gene            8713..9132
                     /gene="ruvX"
                     /locus_tag="PKH58_05505"
     CDS             8713..9132
                     /gene="ruvX"
                     /locus_tag="PKH58_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNX88516.1"
                     /translation="MGRVLAIDYGTKRTGLAVTDPMRIIANSLDTVATHEVLDYLEKY
                     IAREHVDIFVIGLPTQMSGEESDTMKQIRPFAEKLQKKFPAIELKYVDERFTSVLAHQ
                     AMIAGGLKKKDRQNKALVDKISATIILQSYLENNYNI"
     gene            9134..9679
                     /gene="def"
                     /locus_tag="PKH58_05510"
     CDS             9134..9679
                     /gene="def"
                     /locus_tag="PKH58_05510"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNX88517.1"
                     /translation="MILPIYTYGNSVLRKVAEPIDENYPKLKELIKNMFDTMVHAEGV
                     GLAAPQIGLPIRLIVIDLAPLKEDDPELGNFRVIMINPEILEVSEDEVPFDEGCLSIP
                     GINETVLRPESLKISYLDEEFNEHTEIFDGYKARVVQHEYDHLEGHLFTDRIKPLRRQ
                     LIKTKLTNITKGKANCKYKIK"
     gene            complement(9732..10859)
                     /locus_tag="PKH58_05515"
     CDS             complement(9732..10859)
                     /locus_tag="PKH58_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNX88518.1"
                     /translation="MKERQMKNNEYKYILIALMLFLGIIIFKELKSYMSGFLGALTLF
                     VLLRGQMKYLTKKKKWGKGLSSTLIILESLLVFLIPLTGITFLAVDTLSGIKIDPEQI
                     KTAVLGLVSQIETRFDFKIFTPENLSFIPKLGTNLVQMLASSSYSLIINIVIVLFVLY
                     FLLYSYEEFETTIREILPFTPENKQTFVHETKSIIQANAVGIPLLAIIQGIFAYVGYM
                     VFGVSNVLLYAVLTACATIIPVVGTAIIWVPLGVIFLFQGNYIGGIGIILFGAIIIGG
                     VDNLARLILQKRLADIHPLITIFGVFLGIPMFGFWGVIFGPLLLSLFMLFFNMYRHDY
                     ISGSTANPHVTSSYTPVVKQKDKADKKNDEEVKPETEEKKE"
     gene            11377..13923
                     /locus_tag="PKH58_05520"
     CDS             11377..13923
                     /locus_tag="PKH58_05520"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNX88519.1"
                     /translation="MEKKTFTQEDVENATREYFNGDELAAKVWATKYALKDSFGNIYE
                     RTPDDMHHRLANEISRIEQKYPNPLSEKELFELFRNFKYIVPQGSPMTGIGNEHQVAS
                     LSNCFVIGFDGDSDSYGAIIKIDEEQVQLMKRRGGVGHDLTHIRPKGSPVKNSALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLSVAIKHPDAESFIDAKMESGKVTGANVSVKL
                     DDDFMNAAINNLPYTQQYPTNSTNPWVKKECDATALWKKIVHNAWQSAEPGVLFWDTI
                     LKESVPDCYADLGYRTVSTNPCGEIPLCPYDSCRLLAINLYSYVNKPFTKEANFDFEL
                     FSKHVQLAQRIMDDIIDLEMEKIDKIIAKIESDPENDDIKNTEFQLWEKIKTKTSQGR
                     RTGVGTTAEGDMLAAMGITYGTEEAIEFSEKVHKAVALAAYRSSVNMAKERGAFDVYD
                     AKREVNNPFINRIRVADPELYEDMKKYGRRNIACLTIAPTGTTSIMTQTTSGIEPVFM
                     PVYTRRRKVNPNDKNVHIDFVDENGDSWEEFIVFHHKFVTWMEVNNYSTTKHYSKEEI
                     DELIANSPYYKATANDVDWLKKVQMQGRIQKWVDHSISVTINLPNDVSEELVGKLYEE
                     AWRSGCKGATVYRDGSRAGVLVAMEEKKEEKKDDKERCFCIEDERLVRPKELKCDVVR
                     FQNAKDKWIAFVGLYDGRPYEIFTGLADDEEGIVLPKNVTEGKIIKVTDENGKRYDFQ
                     FTNKRGFKTTVEGLSHKFDKEFWNYAKLISGVLRYGMPIDQVIKLVSGLQLDNESINT
                     WKVGVERALKKYIPDGTQISEQACPHCGQKALVYQEGCLTCKNCGYSRCG"
     gene            complement(13985..15025)
                     /locus_tag="PKH58_05525"
     CDS             complement(13985..15025)
                     /locus_tag="PKH58_05525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX88520.1"
                     /translation="MSVSVVILNWNGEKYLNEFLPVLLKNIEDVPGAEVVVADNGSTD
                     GSLQLLSEKFPTVKVDAFDKNYGFAEGYNKALKNINTEYYVLLNSDVEVTKGWLSTLY
                     TYMNENPDVAACQPKILSYHRRNYFEYAGAAGGFIDKYGYPFCRGRIFGEVEEDWGQY
                     DDIKDIFWATGACLFIRSADYWEAGGLDDEFFAHMEEIDLCWRLKSRGKRIVCIPQSV
                     VYHIGGGTLNAEHPRKTYLNFRNNLLLLYKNLPHKSLKHVFDVRFFLDNLAALHLLLQ
                     GKYQNAKAVTDARTDFRTIQSKFSDKRQENILKSVSSLQKEISQHSLIYEFYFKRHKK
                     FSEISPNEELAE"
     gene            complement(15049..>15594)
                     /locus_tag="PKH58_05530"
     CDS             complement(15049..>15594)
                     /locus_tag="PKH58_05530"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX88521.1"
                     /translation="SVILMTSHFGNWEWTSSFSQYLPADKPMYQVYKKLSNKLSDKVV
                     YDLRIRFGSVNVEVDDLFDTLREMRKEGKTGMIGLISDQSPSRKGIRYYSQFLNQRTP
                     VLTGAETIAKIYDFPVYYARIRKVKRGYYSCDPVPICLQPKDSEKFEITEKYIRMLEQ
                     EILTDPSCWLWSHRRWKFANN"
     assembly_gap    15595..15694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15807..16139)
                     /locus_tag="PKH58_05535"
     CDS             complement(15807..16139)
                     /locus_tag="PKH58_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88522.1"
                     /translation="MKTQDDDLLVYDEDDAVKFILKLVPDEQKDRINEDAINYVLDVV
                     YDYYEENGLIDDQSTEEASIDEEEMMKYVLKASKKDKMHLSEDEIQLILDGEYEYGKS
                     LGIYTEED"
     gene            16335..16696
                     /gene="rnpB"
                     /locus_tag="PKH58_05540"
     ncRNA           16335..16696
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKH58_05540"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            16742..>17603
                     /locus_tag="PKH58_05545"
     CDS             16742..>17603
                     /locus_tag="PKH58_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNX88523.1"
                     /translation="MKKLIETIKYLYNFISTDIWRITGHELSRTRRFFYGVIKTIYLA
                     IRGYVRNKLGVRAAALTYSITFAIVPLIALIVAIAKGFGIENYIEKTLQDTFIAQANL
                     IPTVMGFVEKYLKTMQGGIFIGIGVVILLYSVMNLFMQIESTLNNIWQVQKSRSIFKQ
                     FTTYFAGIFLFPVLVGVTSGLSIYINGILKTTFIYQIYSPFAQFSVIVVPYLTSILMF
                     TIIYLIVPNTRVRFLNALIAGILAGIAFQLFQTLYVNGQISLTRYNAVYGGFAAIPLM
                     LLWLNISCQIF"
BASE COUNT         5602 a         3303 c         3436 g         5162 t
ORIGIN      
        1 tcagctgaaa agtacattga aaagaaactt ttatacgtaa acaatgaatt tctacgttta
       61 actgatgcag gcattctgat ttccgatcag ataataactg aactgatgat gtgcggtgat
      121 taataaattc aaaactcatt aacaaatctt ataaaaaaca cctgttgtca aatattaaga
      181 aaaaaataat tgtatttttg catattgaac tgaaaagaaa taaatatggc acttactgac
      241 gattccagaa aaaaaataaa aaaatatttc tggcgctttt tttatacctg tatcggagct
      301 ctggtattga tgtttgcact gataagtatt ggttggttag ggtatcttcc tgaaataaag
      361 gaacttcaaa accctatcaa taaagctgca accgaaatat attcgtcaga catggcgctg
      421 ttgggcaggt tttctttggc caaggaaaat cgggtaccaa ttaattacaa cgaaattgat
      481 aaagacgtaa taaacgcgct gatagctact gaagatgtac ggttttacaa acattccggc
      541 gttgatggca gggctttaat tcgtgtgttc tttggtttgt ttactaattc aaattcgggt
      601 ggtggcagta caataaccca acagctggca aaacagcttt attcaccaag tgccagcaat
      661 attttcaagc gcgctttaca aaaaccaatc gaatgggtaa ttgccgttaa acttgaaaga
      721 atgtattcga aagaagagat aattgcgatg tatctcaatc agttcgactt tctgaacaac
      781 gctgttggta ttaaatcagc tgcgcaggtt tatttcaata ccactgccga caatctgtct
      841 attgaacaag cagctacact tgtgggtatg tgcaagaatc catcctattt caatccggta
      901 aggcatatag aaagaacttt gggacgtaga aatgtagttt ttgatcagat gcgtaaagct
      961 gatatgataa cacgtgaaca acgtgattca ttgagtaaat tacctcttaa acttaattat
     1021 aaacgggttg atcacaaaga aggactcgca acctattatc gtgagtttct acgtcagata
     1081 ttgatggcgg atgaaccaag cagaagcgat tatgccgact ggcagaaaca aaaatacctg
     1141 gatgataaat gggcctggga aaataatcct ttatatggtt tttgcaaaaa aaacaaaaaa
     1201 cctgacggct ccaattacaa tatttatact gatggattga aaatttacac aaccatcgat
     1261 tcacgtatgc agcgctacgc cgaagaagcg gttgaagaac atatgttgaa attgcagaaa
     1321 aacttttttc gcgagaagag aggcagaagt tacgcgccgt tttcgcgtga cttatcacct
     1381 caggaaatag atgatatttt aaaaagagca atgcgtcagt cggaaagata ccgcttgatg
     1441 agcgatgcag gcatgactaa agctgaaatc accaaagcct tcaacacaaa aacagacatg
     1501 caggtattct cctacaacgg aatgatagat accacgatga cacccatgga ttccatccgt
     1561 taccacaagc attttttacg ctgcggattt atgtcgatgg acccaagcaa cggatatgta
     1621 aaagcttatg tgggtggtac gaatattact gcatttcagt atgatatggt aagctcaggg
     1681 cgccgacaga ttggttctac ggtaaaaccc tatttatata cccttgccat ggaagaggga
     1741 atgtcgccct gcgaccgcgt actctgcgca ccaattacca ttcaaacttc atcgggaccc
     1801 tggacaccaa agaactcatc aagcgcccgc atgggtgaaa tggttacttt gcgctggggg
     1861 ctggcaaatt caaacaactg gatatcagca cgtctgatga accaatacac tccggagaca
     1921 ctggttaaac tgatgcagtc gtttggtatt cgcggtcatc tggatccggt cgtttcactc
     1981 gcacttggtc cctgcgaggt gtctgtttcc gaaatggttg atgcatacac cgctttcccc
     2041 tcaaaaggca ttcgtgttga accactatat gttacccgta tcgaagatgc aaatggcaat
     2101 gtaattggtt cttttgtacc aaaaatgcag gaaattttta gcgaaaccac agcatataaa
     2161 atgatttacc tgttgcgaag tgtggttgat gaaggtaccg gtggacgcat tcggagtgtg
     2221 tatggtctca caatgcctgt aggtggtaaa acaggaacaa ctcagaacaa ttccgatggt
     2281 tggtttatgg gtttcacacc gaaacttgtt tcaggcgtat gggttggtgg tgaagaacgt
     2341 tccatacatt ttgatggaat ggctcaggga cagggtgcaa gtatggcatt gccaatttgg
     2401 gctatttata tgaaaaaagt atatgcaaat ccggaactgg gatacagata caacgatcag
     2461 tttgatgtac cggcagattt taatccgaac gaagcctgcg aataacccat accataacgt
     2521 gtccgtttat attttttccg gttttgaaat aaaaataacc attaaacagc tgcacttttg
     2581 aaaaagttaa tttattcagt tctattttta gctatagccc aactgtcatt tagtcagtgg
     2641 aatacggacc gtataatgaa tatcggtaaa aatgcgctgt attatgagga ttttgtcctc
     2701 tccattcagt atttcaatca ggtaattaaa attaagccat acctggctga gccgtatatg
     2761 tacagaggaa tagcaaaaat tagtctggga gattatacgg gagcagaaca ggactgttct
     2821 tcggctattg aaagaaatcc tttcgttccg caggcatatt ttgcgcgtgg ttatgcccgc
     2881 aacaacataa ataaatacga ggaagcaatt tctgatttta ataaagctct ggaattcagt
     2941 ccggaaaata cagatgttat tgctaaccgc ggtgaagcaa aggaaagaat aaacgatttt
     3001 gaaggggcga tcaatgactg gaatctttac cgcaaattga atccgaaatc tcggggcatt
     3061 gattatgaaa tcggacgaat tcagttggct atgaaagata cggtggcagc tatggcatcg
     3121 ctggacaagc atatattatc ggacagtaca aatgctatgg gttacagtat cagggcgctg
     3181 attaaaatgt tgaaaaaaga tgaaaacggt gcttataagg attatagtaa ggccgttgct
     3241 tataaatcga cctatccggg tgactatatt aaccggggaa ttttgaatgt acagcgaaat
     3301 aaattcaatc tggcactgag tgactacaac gaagcaatca ggcttgatcc caaaaacata
     3361 ctggcttatt acaaccgggg tttactgaga gccaacctgg gcgatagcaa caatgccatc
     3421 ggtgatctgg agaaagtggt ggcagccgac accactaatt atgaagcacg tttacaaaag
     3481 gcgtatatgg agctgaaagt tggagatatg actgcggcgg ttaatgattt taacttcata
     3541 ctgaagaaat atccattttt tgtaccggca tattacggaa ttgcagatgc aaaaagtgca
     3601 acaggaagga agaaagaagc tggccagtac cggagtcttg catcagagat aataaacaac
     3661 aaggatttct ataaacgcaa acaaaacctt gtagctaaga atcagattgc aaaagacact
     3721 ccggtcaatg atatgaaaaa atcagttaat cttcttgaaa aactgattgt tagagatact
     3781 gatgatcaaa aatacgagaa taagtacggc gagggtgtcc gtggtgaaat tcaaaacagc
     3841 tatgctgatc tgaaaaatga gcgaaacttt gaactgaatt attttgcgcg ggtacaggaa
     3901 accagaagga cgaatacata tcatccgtta atatcaagat ttaacggcga aaatatgcta
     3961 tcatcatcac tgaaaattac caacgacgta gtaacattta ctgccgatct tatcaatagc
     4021 cggtttgatg aaattaacaa actgacggaa cgaataaaaa acaatccaga agatgccaac
     4081 ttatatttcc tgcgcgctct ggaattctca catgttcagg attattcaag tgctatcgac
     4141 gatcttaacc acgccatcac gctacataca gattttgcct tggcttattt tacacgtgct
     4201 aatcttcgct gtaaaatgat tgaatatcag aattataatc agaatgatga aaaatctgac
     4261 gaaaaaaaca agacaaatat cgcggtaaaa gaagaaagta ataaacttaa ttatgatctg
     4321 ataattcgtg attatgataa aacaacagat ttagctcctg atttttcgtt tgcatattac
     4381 aataaagcta atatcctcag ttctctgaaa aattacaaat ctgccattat tgcttattct
     4441 aaagcaatag aaattgaccc cgattttgca gaagcttact ttaatcgcgg tattgcctat
     4501 ctgtatatga atgatcaggc aaatgctatt ctcgatttaa gcaaatctgg cgaattgggt
     4561 attttccagt catatagcat tatcaaacgt attaagtaaa tctgatttca gttattttct
     4621 acttatctgg tatgatttat gatatttatc atacgacatt tgctattatt caatataatc
     4681 ccaatgattt aactgaatta tttgaatttc tccagcaaaa catgccatag aacatgtatt
     4741 tttaagaaga aaacttgtta tttagaacta tctttgtgtc agcacagcac agaacagtta
     4801 aacctttaga aatgccgttt gttttcaata ataaaagcac aaaagttcag caattggctg
     4861 attaccttac tcagttgata actgttcgtg agtataaggt tggtgaaaag ttaccatcca
     4921 ttaatgagtt gagccgtaaa cacaatgtgt ctagagatac cgtctttaag gcttttctgg
     4981 aacttaaaga aagaggtgtt attgattcgc ttcagggaaa aagttatttt gttgcaagtc
     5041 agaattcaaa tattctgctt gtgctggatg aatatacatc cttcaaagat gttctgtata
     5101 acagccttat taaacgactt cccgaaaaca caaaaatcga tttgtggttt caccagtata
     5161 acgaaaatct ctttaatact attgtgaata gttctgttgg cagatataac caatatctta
     5221 tcatgaacta taaaaacgat gaatattcgc aggttcttga aaaaattgac cgtagcaaac
     5281 tattattact cgattttggt aaatttgata aagacggacg ctcatatatc tgtcagaatt
     5341 ttgatgaaag cttctacgaa gcgctcacaa caataaaaga taacttgaag aaatataaac
     5401 gtttattatt tgttatcaat aagttgcaca gacatcccag aagcagtgtg gaatggttta
     5461 tgaaattttg caacgacaat ggctttttcg gtgagatttg tgaagaaatt tcagaccaaa
     5521 caatagaaaa agatgatttt tacatcattg taaagcagga agatgtcgta aaaatgatca
     5581 aaaagagccg cgtggcagaa ttgaaattgg gcactgacgt cggcttgtta gcatacaacg
     5641 atatgccatt ttacgaaata attgaaaacg gaatttcgag catatccatt gactgggaag
     5701 aaatgggaaa ttatgctgct gagtttattg taaaaaaaga accgatacaa cgttttctga
     5761 aaactaaaat aataaaaagg aattcatttt aatttttttt attaatatat cagcacagta
     5821 cagtatagtg atatttcact aattcattaa aagacttgaa aaccaatcta tgtaactaaa
     5881 tatctctaat caaaaaaaaa tcacaacaat ctctctgtta aactttcccg cctgaccagc
     5941 gggaaagttt ttttatatct atatgatttt ttaaaaagat tgatttttct caaaatccga
     6001 cacatggtat gagttatcta cacagcgtag tattgacatt ttttatattt gtataaaata
     6061 tttattacac tgaattacgt gttgtaaata cttggaatat caactgaatc attatctttg
     6121 ctaagtttta catcgacaaa aatcgaaaga aaaatggcaa tgtacctctc tacattgatt
     6181 gcatttacgg tattaattat cataccggat ctgtggtatt ttcaaaaaat gaaaaaacac
     6241 catttttaca tttggtttcg atatttatca ttaatcccgg gcgtcttttt catcctgtct
     6301 ttattttata tccgtttcgg attggtgtat aataacagct ataaagttac agctgccgtt
     6361 tcatggataa tttttgcatt ttcgattgta tacataccta aattactgta tattatattt
     6421 tattatataa atcactcatt taaccgtata tttaaagccc gaacacgcat atttcgtatc
     6481 ataggatttg ctttttctgt tagtatggtg acagttcttt cgtatggagt agttgtaacc
     6541 cgggatgatt tttatttgaa aaagcagatt atttatgtcg atgatcttcc ggtaaacttt
     6601 gacaactaca gaattgctat gttttcagat ttgcatcttg gcaactggga taataaatac
     6661 atgataatga aacccataat aaaaatgata aactcgggca accccgatat aatcgttttt
     6721 agtggcgata tgatcaataa ctatgagaat gaaaccctgg gctgggaacc ctattttcgt
     6781 caattaaaag ccaggcaggg aatatatgct gtgctgggta atcatgatta cggtgactat
     6841 acaaaatgga aatccgaaaa ggcaaaagcg gagaatcttg aaaaaacaaa aagtaatata
     6901 cgaaatctgg ggtttcgatt attgctcaac gaaagtgtcg aactggtaaa gggttcggat
     6961 acaattacgc tggccggtgt tgagaattac ggttccggtc attttgaaaa ccatggagac
     7021 ctagcagcag cgctgaaagg aactaaacca acacgaaaaa aaatccttat tacccatgat
     7081 cccaatcact gggatgatga agttgtttca aaaagaaagg atatctttct gtcactttcc
     7141 ggacacacgc atgcgggaca aatcgggtat tctaatggta atattcaatt ttcaccttct
     7201 tcggttgtgt atcctcactg ggatggacta tatagcaagg gtagtcagta tttgtacgta
     7261 aatcgaggtg ccggatttgt tggaattccg ttacgtattg gcgtaccacc cgaaattact
     7321 ctgatagttt tgaaaagaaa gcataagaga attgaaagct gagactaata ctctctggtt
     7381 ccgaaaatca aactgccaat ccgaaccatt gtactgcctt cctcaatggc aattttataa
     7441 tcatccgaca tgcccatgga tagttcgcta aaacaatcat caccagtaaa ataatccttc
     7501 ttcaattctt caaaaagatg tttcaatgac ctgaattcat ccctaatctg tgatttgttt
     7561 tcggtgagtg tagccatacc catcaatccc ctgatttcaa tatttttaaa ggttgggaat
     7621 tcaaagttgt tcagcatttc atgaatttct tcaaccgaaa aaccgaattt ggtttcttcc
     7681 tgagcaatat gtatttgcag caagcagggt actaccctac ccgccttgta tgcctgattg
     7741 ttgatttcct ttaaaagttt gaaactgtca acaccatgaa taagtgaaac gaacggtacg
     7801 atatacttaa ttttattcga ctgcagatgt ccgataaaat gccaatggat atccgacata
     7861 agctgagcct gtttggccgt aagctcctgt acgcgacttt ctccaaaaat tctttcccca
     7921 caagcatagg cttccctgat tgcactttca ggatgaaatt ttgaaacaca tacaagttgc
     7981 acatttcctg gaatatttct cctgatttca gtaaggtttt gcaaaatgct cataagtttg
     8041 ttaatgaaat aatcaaattt gctgttaatt gcgaattatt cagctttata tggaaaaaca
     8101 tctaaaatag ttgtttcagc aattgccgca atatcatagt cggccagtgt gtctttcatc
     8161 cctttttcaa tcacttcaaa ggcatctttt agggttgaag cctgagcaaa catagttgtg
     8221 gcaattcttt tttcaattcc cttttcctcg tccagactga tgaaatttac ttttgcccga
     8281 taccagcgat cgccgttttc gttggtgaaa agttcattga tttttgctct tttgatgtca
     8341 ctaaccagaa actcgccgct gataaacgga cgcatctctt cgttgatccg ttcttcggct
     8401 tcggtaaatg acaaggcatc aaccagatat gcttcactta ctttgattac acctccgtct
     8461 tcagcatttt tctcgtattt aattttacat tggaaccaat tatgcatttt gttaattatt
     8521 tgtttgttag tatgttatgt ttgttgcgat ttatttttcg cggactacaa aagtagcaaa
     8581 aaataacaga actataaaca ttgcggttca ccaaatacac aaaataatga taaaacgaca
     8641 catgctgagt tgtatgttgt taagaattct atgtgaagtt ttttttgtac ctttgcagaa
     8701 aatttcgaca atatgggtag agttcttgca atagattacg gcactaagcg aaccggtttg
     8761 gcggtaaccg atcccatgcg cattattgcg aacagtctgg atactgtggc cactcacgaa
     8821 gtgcttgatt atctcgagaa atacattgcc cgtgagcatg tggatatatt tgttatcgga
     8881 ctaccaactc agatgagcgg tgaagaatct gatacaatga aacaaatcag accttttgcc
     8941 gaaaaattac aaaaaaaatt ccctgctatt gaactgaaat atgtcgatga gcgtttcaca
     9001 tcggtacttg cgcatcaggc aatgatagcc ggtggattaa agaaaaaaga tcgtcagaat
     9061 aaagcgcttg tggataaaat aagcgctact ataattttgc aaagctatct ggaaaataat
     9121 tataatatat aagatgatac tcccaattta cacgtatggc aattcggttt taagaaaagt
     9181 tgctgaaccg attgatgaaa attacccgaa actcaaagaa ttgataaaga atatgttcga
     9241 caccatggtt catgccgaag gggttggact tgccgcgcca caaataggtt tgccgatacg
     9301 gctgattgta attgatctgg ccccactgaa agaagatgat ccggaattag gaaatttcag
     9361 ggtaatcatg attaaccccg aaatacttga agtgagtgaa gatgaagttc ctttcgatga
     9421 aggatgtctg agtatcccgg gcataaatga aactgttctg cggcccgaaa gtctaaaaat
     9481 aagctatctg gatgaagaat tcaacgaaca cacggaaatt ttcgatggtt acaaagcacg
     9541 tgtagtgcag catgaatatg atcatctgga agggcattta tttaccgacc gaatcaaacc
     9601 actgcgacgt cagttgatca aaacaaagct gacaaatatc acgaaaggga aagcgaactg
     9661 caaatacaaa atcaaataag tgcttttaaa attaaaacgg ggtgaatgtt tttagcattc
     9721 gccccgtttt tttattcttt tttttcttcc gtttccggtt ttacttcctc atcgtttttt
     9781 ttgtcggctt tatctttctg tttcacaacc ggagtataag aacttgtcac atgcgggttg
     9841 gctgttgaac cagaaatgta atcgtgccgg tacatattaa agaaaagcat aaacaacgac
     9901 aacaataacg gcccgaagat cactccccag aagccaaaca tcggaatacc caggaataca
     9961 ccaaaaatag taatcaaagg atgtatgtca gctaagcgtt tctgcagaat cagacgggct
    10021 aaattgtcaa cgccaccgat gataatcgca ccaaacaaaa ttatacctat acctccgata
    10081 taattgccct gaaataaaaa aataacaccc agcggcaccc agatgattgc tgtacccaca
    10141 accggaatga tggttgcgca ggctgtaagt actgcatata gtaagacgtt gctaactccg
    10201 aaaaccatgt aacccacata ggcaaaaatt ccctgaatta tagccagcaa cggtatcccg
    10261 acagcatttg cctgaataat ggattttgtt tcatgaacaa aggtttgttt gttttcagga
    10321 gtgaatggca gaatttcgcg aatagtcgtt tcgaactctt cgtaactgta tagcaaaaaa
    10381 tacaacacaa aaagcacgat tacaatattg ataatcagtg aatatgaact tgaggcaagc
    10441 atctgtacca ggtttgttcc cagttttgga ataaacgata aattttcagg agtaaaaatt
    10501 ttgaagtcaa agcgggtttc gatttgtgaa actaatccaa gcaccgctgt ttttatctgt
    10561 tccggatcaa ttttgatgcc cgagagtgta tccacagcca gaaatgttat tccggtgagt
    10621 ggtatcaaaa acaccagcag cgattccagt atgattaatg ttgaacttaa tcctttaccc
    10681 catttcttct ttttggttaa atatttcatt tgacccctaa gcagaacaaa aagcgtcaga
    10741 gccccaagga agccactcat atatgatttg agttctttaa aaataataat tcccagaaaa
    10801 agcatcaaag caataagaat atacttatat tcattatttt tcatttgtcg ttctttcata
    10861 caacatatat attaatgaat taatacgggt tattgggtga ttcaatcctg atgaaattcc
    10921 ggattttcaa tgtgtaaagt tacaataaaa ttcatttctg taatccgata aagataaatt
    10981 ttgtaaaatc atttcctggt acaaaaaatg ggaatgagaa tttagaatac aatccctgta
    11041 gacaaacgaa acttaccgga aaatattttt tccggtttct ctatccgact gaacaacata
    11101 cctataacaa acttccggta taatataata aaatttattt aatcttccag atgtttcatt
    11161 agaaaaaatc attttagaaa acattttttg ttttataaaa atttatagtt cactgtatca
    11221 gctaaataaa aattattttg gaaaacatcg aaaaatattt cgaaaatata tttgtcgatt
    11281 ataggaaagt tcggaaatat tttcctaaaa ttgtattctg aaaaataagc ggactaaaat
    11341 ttcataatta attaacatat aatacaataa cgtatcgtgg aaaaaaagac ttttactcag
    11401 gaagatgttg aaaatgctac cagggaatat ttcaatggag atgaactggc tgccaaagtc
    11461 tgggcaacga aatacgcctt aaaagactca tttggaaaca tttatgaaag aactccggat
    11521 gatatgcatc atcgtttggc taacgaaatt tcaagaattg aacaaaagta cccaaatcct
    11581 ctgtccgaaa aggaactctt tgaattgttc cgtaacttca agtacatcgt tcctcaggga
    11641 agtccgatga ccggaattgg aaacgaacat caggtagcct cattatccaa ttgctttgta
    11701 ataggttttg acggagattc tgattcatac ggcgccatca ttaaaattga tgaagagcag
    11761 gttcagttga tgaaacgccg cggaggcgtt ggccatgacc tcacccatat acggcccaaa
    11821 ggttctcccg taaaaaactc agcgcttact tccacaggta ttgttccctt catggaacgg
    11881 tattcgaatt caacccgcga ggttgcgcag gacggacgcc gtggtgctct aatgcttagt
    11941 gtggctatca agcatccgga tgccgaatcg tttatcgacg ctaaaatgga atcaggtaag
    12001 gttacaggtg caaatgtatc agtaaaactt gacgacgatt ttatgaacgc cgctataaat
    12061 aatcttccat acacgcagca atatccaaca aacagcacta atccatgggt aaagaaagaa
    12121 tgtgacgcaa cagctctttg gaaaaaaatt gttcataacg cctggcaatc ggctgaaccc
    12181 ggtgtacttt tctgggatac cattctgaaa gaatctgtac ctgattgcta tgcagatctg
    12241 ggttaccgca ccgtatcaac aaatccatgc ggcgaaatac cgttgtgtcc gtacgacagc
    12301 tgccgtttgc ttgccataaa tctgtattcg tacgtaaata agccatttac taaagaagca
    12361 aattttgatt ttgaactttt ctctaagcat gttcagctgg ctcaacgcat catggatgat
    12421 atcattgatc tggaaatgga aaaaattgac aagatcattg cgaagataga atcggatccg
    12481 gagaacgacg acatcaagaa caccgaattc cagctttggg aaaaaatcaa aacaaaaaca
    12541 tcgcagggac gtcgtacagg tgtgggaact acagccgaag gcgatatgct cgcagcaatg
    12601 ggaattacct acggtactga agaagccatt gagttttcgg agaaagtaca caaggcagta
    12661 gcactggctg cttaccgttc gtccgttaat atggcaaagg aacgtggggc tttcgatgtt
    12721 tatgacgcaa aaagagaagt aaataatcct tttatcaacc gtattcgtgt agctgatccc
    12781 gaattgtatg aagatatgaa gaaatacggt cgtcggaata ttgcctgctt aaccattgct
    12841 cctacaggta ctaccagcat catgactcaa acgacatccg gtattgaacc ggtgtttatg
    12901 ccggtataca cccgcagacg caaagtcaat ccaaacgaca agaatgttca tatcgatttt
    12961 gttgacgaga acggagactc gtgggaagaa tttatcgtat ttcatcacaa atttgttacg
    13021 tggatggaag taaataatta ttcgactaca aaacattaca gcaaagaaga aatagacgaa
    13081 ttaattgcaa attctcctta ttacaaagct acagctaacg acgttgactg gcttaaaaag
    13141 gttcagatgc agggtcgcat ccagaaatgg gtggaccact ctatcagtgt taccatcaat
    13201 cttccaaacg atgtttcgga agaacttgta ggaaaacttt acgaggaagc atggagatcc
    13261 ggatgtaaag gtgcaactgt ttatcgcgat ggttcccgtg ccggagttct tgtagcgatg
    13321 gaagagaaaa aagaggagaa aaaagatgat aaggagcgtt gcttctgcat tgaagatgaa
    13381 agacttgtac gtccgaagga attgaaatgc gatgtggtac gtttccagaa tgcgaaggat
    13441 aaatggattg cttttgtagg cttgtatgat ggaagaccct atgaaatttt caccgggctt
    13501 gctgatgacg aagaaggaat tgtattgcca aagaacgtta ccgaagggaa aatcattaag
    13561 gttacagatg aaaacgggaa acgttatgac ttccagttta ccaacaaaag aggatttaag
    13621 actactgttg aaggactttc gcataagttc gacaaagaat tctggaacta cgcaaaactg
    13681 atttccggtg tattgcgtta tggcatgccg atcgaccagg ttatcaaact ggtatccggt
    13741 ttacaactcg acaacgaatc tatcaatacc tggaaagtgg gtgtggaaag agcgctcaag
    13801 aaatacattc cggacggaac gcaaatttcg gaacaggctt gtccgcattg tggtcagaaa
    13861 gctctggtat atcaggaagg atgtttaacc tgtaaaaact gtggttactc acgttgtgga
    13921 taatcttaaa aacatacatt catcaataaa aaagacggga atttcccgtc ttttttattg
    13981 atgattattc tgccaactct tcatttggcg atatttcgct gaacttttta tgtctcttga
    14041 aataaaactc gtagatcagg ctatgctggg agatttcttt ttgtaacgaa ctgactgatt
    14101 tcaaaatatt ttcctgacgt ttatcggaaa atttcgattg aatggttcgg aagtctgttc
    14161 tggcatcggt tacagctttg gcattctgat acttgccctg cagcaacaaa tgcagtgccg
    14221 ccaggttgtc gagaaagaag cgaacgtcaa agacatgttt caggcttttg tgaggaagat
    14281 ttttataaag cagcaacagg ttgtttctga aattgagata ggttttacgc gggtgttcgg
    14341 cattcagagt accaccgcca atgtgataca ctacgctttg cggaatgcat acaatgcgct
    14401 taccacgact tttcagccgc cagcaaaggt caatttcttc catatgagca aaaaactcat
    14461 catcgagacc accggcttcc caataatcgg ccgaacggat aaacagacag gctcctgttg
    14521 cccagaaaat atcctttata tcgtcgtatt gaccccagtc ttcctctact tcaccaaaaa
    14581 tacgtccccg gcagaacggg taaccatact tatcgataaa cccgccggca gcaccggcat
    14641 attcaaaata attccggcga tgataagaca ggattttagg ttgacaggca gccacatccg
    14701 gattttcatt catataagta tatagggtcg agagccagcc ttttgttact tcaacatccg
    14761 aattcaacaa cacataatat tccgtattta tgtttttcaa tgctttgttg tacccttcgg
    14821 caaaaccata attcttgtcg aaagcatcca ctttcactgt tgggaatttt tcggagagaa
    14881 gctgcagtga accatcggtg gagccgttgt cagctacaac aacttcagcg ccgggtacat
    14941 cttctatatt tttcaggagg actggcaaaa actcattcag atatttctca ccattccagt
    15001 ttagtattac tactgataca gacattttat tttgttattt attagagatc aattatttgc
    15061 aaatttccat cggcggtgcg accaaagcca gcaggaagga tcagtcagga tttcctgttc
    15121 gagcatgcgg atgtatttct ctgtaatttc aaatttctca ctatccttag gttgcaaaca
    15181 aatcgggaca ggatcacagc tgtagtatcc tcttttcact ttacggatcc gggcataata
    15241 aacaggaaaa tcatagattt ttgcaatcgt ttcagcgcct gtgagcaccg gtgttcgctg
    15301 attcaaaaac tgcgaataat atctgatacc ctttctcgaa ggactttggt ctgaaatcag
    15361 gccgatcatt ccggtttttc cttcttttcg catctcgcgg agtgtatcaa acaaatcgtc
    15421 aacttccaca ttgacagaac caaaacggat gcgcaggtca taaacaacct tatcggaaag
    15481 tttgttcgaa agttttttat acacctgata cattggttta tccgcaggaa gatattgact
    15541 gaatgatgaa gtccattccc agttgccaaa gtgagaggtc atgagtataa cactnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagaaa tcagttttat tattaccctt
    15721 gtaaaatgat acatatgctt attaataacc aaaaaaacac ccgacaataa atgtcagggt
    15781 gtttttttgc atacattctt ataattttaa tcctcttcgg tataaatccc gagagatttt
    15841 ccatattcgt actcgccatc gagtatcagt tgaatttcat cttccgagag atgcatttta
    15901 tcctttttcg atgctttcag cacgtatttc atcatttctt cctcatcaat acttgcttct
    15961 tcggttgatt ggtcatcaat cagtccgttt tcttcataat aatcatatac cacatccagt
    16021 acatagttga tagcgtcctc gttgattctg tccttctgtt cgtcaggaac cagtttcaga
    16081 ataaatttca cggcgtcatc ttcgtcataa accagtaaat cgtcgtcctg tgttttcata
    16141 attatatatt ttaaattatt aaatttactt tcaaaaatct gtttcaaaga tacatgattt
    16201 tcacaacact atttattatt ttagttttta ttatcaatta atctgtcggt taaaatattt
    16261 cttaaatatc gaattatact gttatattct tattattttg cagattatgc aaaatcgctt
    16321 atctttgtat ataaatagca gaccggtttg tcgctgtctc actcacgtga gattgaggaa
    16381 agtccgggca acgcagagca ccatatttcc taacgggaag ctgttcgaaa gggcagagta
    16441 gtgtaacaga aaataaccgc ttcgtttgtg acggagtaag ggtgaaaagg tgaggtaaga
    16501 gctcaccggc tgtggtggca acatcgcggg ccgtacatct tatgggttgc aagatcatgt
    16561 ataccggcgc agtaggactg ctcgtccgag ccggggggta gattgctaga tcccgtcagt
    16621 gatgacagga ccagataaat gacaaacact ccgcatctgc ggagaacaga acccggctta
    16681 caggtctgct tcatttgtaa aattcctgtt gcatgaagta tgcaataaca actaatattc
    16741 aatgaagaaa ctaattgaaa ccatcaaata tctttacaat tttatcagta cggatatatg
    16801 gcggataacg ggtcacgaat tgtcacgtac ccgtcgtttt ttctatggtg tcatcaaaac
    16861 catctatctg gctatcagag gttatgtgcg aaacaaactc ggtgtccggg cagcagcact
    16921 cacctactcc attacatttg ccatcgtacc gctcatcgca ctgattgtag ccattgccaa
    16981 agggttcgga attgaaaact atatcgaaaa aacacttcaa gacactttta ttgctcaggc
    17041 aaacctgata cctacggtga tgggatttgt ggaaaaatac ctgaaaacaa tgcagggagg
    17101 aatatttatc ggaattgggg ttgttattct tttgtactcc gttatgaacc tttttatgca
    17161 gatagaatca actctgaaca acatctggca ggttcaaaaa tcacgctcca ttttcaaaca
    17221 atttacaacc tattttgccg ggattttttt atttccggta ttggtcggag ttacaagcgg
    17281 actttccatt tacatcaacg gcatactaaa aaccactttc atttatcaga tatatagccc
    17341 gtttgcgcag ttttcagtta tagtcgtacc ctacctgacc agtatcctga tgtttaccat
    17401 catatatctg atcgtaccta acacgcgtgt caggtttctc aacgcgctga ttgccggaat
    17461 acttgccgga attgcatttc agcttttcca gacattgtat gttaacgggc aaatcagcct
    17521 gacacgatat aatgccgttt atggtggttt tgcagccatt ccgttgatgc ttttatggtt
    17581 gaatatctcc tgtcagatat tcc
//
LOCUS       DAOPYB010000049        17239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3498_length_17239_cov_9.286196, whole genome shotgun sequence.
ACCESSION   DAOPYB010000049 DAOPYB010000000
VERSION     DAOPYB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17239
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3498_length_17239_cov_9.286196"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..>1401)
                     /gene="rsgA"
                     /locus_tag="PKH58_05550"
     CDS             complement(311..>1401)
                     /gene="rsgA"
                     /locus_tag="PKH58_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNX88524.1"
                     /translation="DIRSHQSSAMNLEDLGYTGQLEKFRLENNLSDFEIGRVISEHKE
                     RYIVRTEKGELEAEITGNLRYSARSREDFPAVGDWVALTIYDSDYSIIHRVLPRSSVI
                     CRQAVGETGEIQIIAANIDYALLVQAVDRDFNINRLERYLTLCNSSRINPVIVLTKTD
                     LIDRQRTFELMEKIQERIKQVPVIAISNESRDGYDKIKSIFEKGKTYCMLGSSGVGKS
                     TLLNNLSGKSIMRTDHISVSTHKGRHITSHRELIILESGGLLIDNPGMREVGIADSAN
                     GLNITFDTIVKLSHECRYNDCTHIHEAGCAVIEAVKKGEIDKASYENYLKMEREKAYY
                     EATLAEKRKKDKYFGKIIKNYKRDRDSL"
     assembly_gap    1402..1501
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1520..2863)
                     /locus_tag="PKH58_05555"
     CDS             complement(1520..2863)
                     /locus_tag="PKH58_05555"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNX88525.1"
                     /translation="MEHIKLSIDKAYGFVSAEKVAAYKAEAEAANLALQNGTGKGSDF
                     LGWLNLPSEIDEAHLTDVENTAKILRDNCEVVVVIGIGGSYLGAKAVIDALSNSFDWL
                     QTERTSPVIVYAGQNIGEDYLYELQELLKTKRFGIISISKSGTTTEPALAFRLLKTQL
                     EEQQGKEEAKNLIVCITDKAKGALRVLANQEGLKTFILEDNIGGRYSVLSPVGLLPIA
                     VAGLDIRQLVQGAVTMQKVCGTGTAFEENPATQYAAVRNELYRDGKKTEILVNFHPKL
                     HYVSEWWKQLYGESEGKENKGIFPASVDFTTDLHSMGQWIQEGERTIFETVISVCEPN
                     HTKIFPHSDENLDGLNFLAGKRVDEVNKMAELGTMIAHVDGGVPNLKIELPKLNEFYL
                     GELIYFFERACGISGYLLGVNPFDQPGVEAYKKNMFALLEKPGYEEETKAIKARL"
     gene            complement(2876..3949)
                     /locus_tag="PKH58_05560"
     CDS             complement(2876..3949)
                     /locus_tag="PKH58_05560"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNX88526.1"
                     /translation="MITESIEWGNTNISLKDILTNAPDKKHVEKVRIAIMGGGSWATA
                     LAKIALTNIKSINWYMRRQEQIDEFIRTSRNPSYLTGVRFNTDRIRFTTDINKVVKMS
                     DILVFATPSPFLKQHLSKLKRHLDDKIIVSAIKGIVPDENMLISEYFHEKYKVPEENI
                     VALGGPCHAEEVALERLSYLTIACKSRDNARYIAEKFTNDYIHARISDDVYGLEYTSV
                     MKNIYAIASGICSGLKYGDNFQAVLISNAVQEMNRFCNATNPQHRDISESAFLGDLLV
                     TAYSKFSRNRLFGTMIGKGYSVKTAQLEMEMIAEGYYATKTIHELNEKYHVNIPIVDA
                     VFEILYNRSSPILEIQQLTEKLQ"
     gene            complement(4055..5788)
                     /gene="lysS"
                     /locus_tag="PKH58_05565"
     CDS             complement(4055..5788)
                     /gene="lysS"
                     /locus_tag="PKH58_05565"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNX88527.1"
                     /translation="MSIELSEQEQFRRQSLTELRKLGIEPYPAAEYPANAFSTDIKAE
                     FKDEDTGKNVCIAGRIMSRRIMGKASFVELQDSKGRIQVYVSRDDISTEAQPEMYNTV
                     FKKLLDIGDFIGIKGFVFRTQMGEISVHAQEMTVLSKSLKPLPIVKYKDGVAFDAFED
                     PEQRYRQRYVDLVVNEGVKDIFIKRTKVYQSMRDYFNSKDYLEVETPILQPIPGGAAA
                     RPFITHHNALDIPLYLRIADELYLKRLIVGGFEGVYEFSKNFRNEGMDRTHNPEFTAM
                     EIYVAYKDYNWMMTFTENMLEKICLDVNGTTEVKVGDNIINFKTPYKRISMIDSIHEF
                     TGIDINGMNEEQLFEVCKKLDIEVDDTMGKGKLIDEIFGAKCEGNYIQPTFITDYPRE
                     MSPLCKRHRENPELTERFELMVNGKELANAYSELNDPIDQLDRFQEQLKLSEKGDDEA
                     MFIDLDFVRSLEYGMPPTSGMGIGMDRLVMLMTGQQTIQEVLFFPQMKPEKVITQDTD
                     DKYIALGIPAEWIEVVKKAGITKVRDLRGLNPNKFHQDICGLNKKNKLELKNPAKEDV
                     QAWIAAAGNLA"
     gene            complement(6084..8318)
                     /locus_tag="PKH58_05570"
     CDS             complement(6084..8318)
                     /locus_tag="PKH58_05570"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX88528.1"
                     /translation="MRKSIFTLILFFLTSITVGFAQKAHHWETAVFKSDIWKYFVGTS
                     NPGINTNPSWRALTYNDTGWLSGSGGFGWSDNDDGTTIPTCTSVYLRIKFNIPDTSQI
                     ILGALDMDYDDGFVAYINNVEIARANLGTVGVYPSFSTSATASHEATMYQNEGTGMPE
                     QFVIEKKTLLQCIKPGINILAVEVHNYTKSSPSDLSSNVFLSFGIKNATTIFRSTPDW
                     FIEPEIISPLDYTSSYLPLIVINTKNGATIVDEPKIEADMKIIHHGDTARNHLTDSAN
                     VYNGKIGIEIRGATSAGFPQIPYGLETRDSLGENLNVKLLGMPKENDWVLLSNWNDKV
                     YMRNTLAGKIFEEMGHYAPRLKHCEVTINGAYRGLYLFGEKIKIDDNRVDIAKLKSTD
                     LAGDSLTGGYIFKTDYDEGTGTYWTSNFSPANRPGALVYFVYHEPKANEIADEQKTYI
                     TSYVNAVETALYSSDFNNPVTGYRAYIDVNSFVDYWILEELSRNVDGYKKSRYFYKDK
                     DSKDRRLHSGPIWDFDWAWKNMDDCYLYKNTTGEGWAYDINKCEDTDPIPPSWEYRML
                     QDPMFANAIHDRYFSLRASILSLDYMYHYIDSVASVLNEAQERHQSIYNTLGIGNGSP
                     EIDDQPTTFEGDITKFKNWIATRIAWLDANMVGISTDDTALKSDFILRVFPNPVQEIL
                     YIESDQNISDIKLYNVAGLVMMDIKPNINSKALNIRQLPRGIYILHVTLDNGNKLAQK
                     LIRQ"
     gene            8484..9914
                     /locus_tag="PKH58_05575"
     CDS             8484..9914
                     /locus_tag="PKH58_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNX88529.1"
                     /translation="MSSLAELFEKQTGTKPVSEVQLTVSGSNRKYYRLKNDTISLIGV
                     IGTSVEENRAFIAMAQHFRKKGLPVPELGEVAADEMMYLQEDLGDAVLFDYLVNSRKT
                     GVFSSEEKAFLHKTMEMLAKFQVVGAQGFDFSVCYPQPEFNERSILWDLNYFKYCFLK
                     STGLDFQENKLEDDFEKLAKELLKAKTETFMYRDFQSRNVMLKDNEPYFIDFQGGRKG
                     PLYYDVASFIWQAKAQYGPQLRDELVDAYLHSLKIYMEVDEDEFRRVLDHFVLFRLLQ
                     VLGAYGFRGYFEKKPHFLQSIPYALENLRALMKNNFKEYPYLMKVLREMSELKQFKDV
                     RLHRPLVVTVYSFSYKKGIPHDISGNGGGFVFDCRAVNNPGKYERFYSMTGLDEPVIQ
                     FLENDGEILKFLNSAYSLVDASVKRYKDRGFTNLMICFGCTGGQHRSVYSAQKMAEHI
                     HDQFGVEVNLIHRERNLEQTFEETKD"
     gene            9986..10528
                     /locus_tag="PKH58_05580"
     CDS             9986..10528
                     /locus_tag="PKH58_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HNX88530.1"
                     /translation="MNTTSQQFDQVTAVCRDIFTKKLKDYGASWRILRPRSVTDQIFI
                     KANRIRSIETKGVNKVGEDIGGELIAIVNYGIIGLIQLELGFAEKDDLSIEDALKLYN
                     KYLEQAKNLMADKNHDYDEAWRSMRTESYTDMILMKIYRTKQIEEHDGKTLISEGIDA
                     NYYDMINYAVFGLIKMKFKD"
     gene            10558..11709
                     /locus_tag="PKH58_05585"
     CDS             10558..11709
                     /locus_tag="PKH58_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNX88531.1"
                     /translation="MYQQHQRSRSVAKSEVYTLKKTGKILLWLARMIVGITFVFSGFV
                     KAVDPLGSAYKFEDYFTAFGRFFEHLTPIALPAAIALSTLELLIGFCFLLKIKMKGMS
                     VVALLFMAVMLPLTLYIALNNPVSDCGCFGDAIILDNWTTFFKNIILSLLIVVVMIFV
                     KQQQPIFLPAAEWIVITLVAIAGIGFSVYNYRHQPVIDFRPYKIGVNIPEKMVIPEGA
                     EPDKYETMLVYEKDGQQKEFTIENYPKDSTWTFVEQKTTLVSKGYTPPIHNFQIVDQN
                     LNDITDYVLSYKGTVNLLCMYDLNKTSEKGVKAAEVFYQKIKSKGTPFYALTASSDEE
                     IEAFRNRTGATYDFCKTDPITLKTIVRGNPALMIIKNGTVVGKWNWRDF"
     gene            11807..12562
                     /gene="tpiA"
                     /locus_tag="PKH58_05590"
     CDS             11807..12562
                     /gene="tpiA"
                     /locus_tag="PKH58_05590"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNX88532.1"
                     /translation="MRKNIVAGNWKMNKTLQEGVALAAELKDVLAGKTPKCEVVIGTP
                     FIHLATVANQVEGSVIKVAAQNCANKESGAYTGEVSATMVKSTGATYVILGHSERREY
                     YGETSAILNDKVALALSNGLTPVYCCGEALEVRNANTQNEFVKNQLEETIFNLSAEDF
                     QKLVIAYEPIWAIGTGVTASTEQAQEMLAFIRSLIADKFGKEIAENTSILYGGSCNAK
                     NAPELFAQPDIDGGLIGGASLKAADFVAIIDAF"
     gene            12658..13263
                     /locus_tag="PKH58_05595"
     CDS             12658..13263
                     /locus_tag="PKH58_05595"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNX88533.1"
                     /translation="MLIKIYNDNPNPNQIRHVAEVLRNGGIIIVPTDTVYAFGCDIFN
                     SAAVEFISKLKNKDLRKSELSFICHEISQISEYAKLDDDSFKLIKKNLPGPFTFILNG
                     SSRLPKLFKNKKTVGIRMPDNKITLQIVRELGNPMMTSSIFVDDATTEYITDPELIDE
                     KYGHQVDLVVDGGMGGLLHSTVVDCTGDQAEIIREGFGELQ"
     gene            13529..13984
                     /gene="rplM"
                     /locus_tag="PKH58_05600"
     CDS             13529..13984
                     /gene="rplM"
                     /locus_tag="PKH58_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNX88534.1"
                     /translation="MDTLSYKTISANKATVQKEWVVVDATDMVLGRMGSKVAKLLRGK
                     YKPSFTPHVDCGDNVIIINADKVKLTGNKWTDRVYLRHTGFPGGQREYTPSDLMAKDP
                     DRLIRKVVRGMLPKNRLGDKIINNLYVFAGAEHNMQAQKPKQIDLNSLK"
     gene            13990..14376
                     /gene="rpsI"
                     /locus_tag="PKH58_05605"
     CDS             13990..14376
                     /gene="rpsI"
                     /locus_tag="PKH58_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNX88535.1"
                     /translation="MEVINALGRRKAAIARVFVSEGKGQITINKRDLAVYFPSPILQY
                     VVKQPLNKLGVAEKYDIKVNLDGGGYKGQAEALRLAIARALVKIDPECKKSLKVEGFI
                     TRDPREVERKKPGRPKARKRFQFSKR"
     gene            14552..15385
                     /gene="rpsB"
                     /locus_tag="PKH58_05610"
     CDS             14552..15385
                     /gene="rpsB"
                     /locus_tag="PKH58_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNX88536.1"
                     /translation="MSRTNFEELLEAGCHFGHLKRKWNPAMAPYIFMERNDIHIIDLH
                     KTVVKIDEAAAAMKQIAKSGRKILFVATKKQAKDVVADKATSVGMPYITERWAGGMLT
                     NFPTIRKAVKKMTTIDKMSTDGTFDNISKREKLQITRQREKLEKNLGSIADLTRLPSA
                     LFVVDVMKEHIAVKEAQRLGIPVFGIVDTNSNPNDIDFVIPANDDATKSVEVILNAMC
                     AAIQEGLEERKIEKIDAEAAEAPEVKEKKSRVSKRPRTQKEDDEALKANVVKKFVKEE
                     E"
     gene            15559..16563
                     /gene="tsf"
                     /locus_tag="PKH58_05615"
     CDS             15559..16563
                     /gene="tsf"
                     /locus_tag="PKH58_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNX88537.1"
                     /translation="MAVTMAEISKLRTMTGAGMMDCKNALVEAENDFEKAIEIIRKKG
                     KAIAAKRSEREASEGCVLAAAKDGFAAVLALKCETDFVAKNADFIALTQTILDKALAE
                     KPESIDALKALTITEDGRTIADLVTERSGVTGEKMEVDYYEFVKGGTVTSYIHPGNKL
                     ATIVAFAENDAEYETIHGVAMHIAAMSPVAIDESAVPQKVKDNELEVAIEKTKIELVS
                     KEVEVALKKAGINPAHVDSEDHIESNMAKGWITAEDAAKAREIKTTVFAEKSATLPEQ
                     KVNSIAAGRMAKFYKEYTLVEQKYEGGGDEAGKTTVKELLAKKHLTCIAFKRVTLNQE
                     "
     gene            16735..>17239
                     /locus_tag="PKH58_05620"
     CDS             16735..>17239
                     /locus_tag="PKH58_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNX88538.1"
                     /translation="MDKKVRVRFAPSPTGPLHIGGVRTALYNYLFARHFGGDMLLRIE
                     DTDSARFVPGAEEYIVEALQWLGITIDEGIGAQTAGKHAPYRQSERKEIYKKYVDQLL
                     AEGKAYLAFDTPQELDFQRGKINNFQYDATTRGFMQNSLTLTKEEVKKRLADGEQYVV
                     RIKIEPNI"
BASE COUNT         4941 a         3415 c         3678 g         5105 t
ORIGIN      
        1 tcgaagcgct gaggttcttc acgtccatgc ctaacctgcc atccttgatt attttctttt
       61 tgttgacgtc gctttcggct gtgtttttca gggctggtga agcctctttt ttatcacttt
      121 ccgacttcat taaaacgtcg ctgctatatt ctgctgactt tggagcgcag gaagcaacta
      181 aaaatacaat aaggattatt ctgacaattt gttttataag gttgaagagt gttgggaatg
      241 agtaaattac aattagttta cattcactgt gtttagatag ttatattttt tttctgaatt
      301 gttggtcatg ctacaaacta tcccggtctc tcttgtagtt tttgattatt tttccaaagt
      361 atttgtcttt ctttcgtttc tcagccagag tggcttcgta ataggctttt tcccgttcca
      421 ttttcaggta attttcgtag gaagctttgt ctatttctcc ttttttgact gcttcaataa
      481 ctgcacaacc agcttcgtgt atatgggtgc aatcgttgta cctgcattca tgcgaaagtt
      541 ttacaatggt gtcgaatgta atattcagtc cgttggctga gtcggcaata cctacttctc
      601 tcatcccggg attgtcaatc aaaagaccac cgctttcaag aataatcagt tccctgtgac
      661 tggtaatgtg ccgacctttg tgagtgctca cgctaatatg gtcggttcgc atgatacttt
      721 tacctgaaag gttattgagt aatgtggatt ttccaacacc tgacgaaccc agcatgcaat
      781 aggtctttcc tttttcgaat atcgatttta ttttatcgta accatcccgc gactcgttgc
      841 tgatggcaat aaccggaact tgtttaattc gctcctgaat tttctccata agctcaaaag
      901 tccgctgcct gtcgattaag tcagtttttg tgagtactat aacaggattg attcgggatg
      961 aattgcaaag agtgaggtat ctttcaagcc ggttgatatt gaaatcgcgg tcgactgcct
     1021 gcaccagcaa tgcataatca atattagcag cgatgatttg tatctcaccc gtctcaccga
     1081 cagcctgacg gcaaatgaca gacgatctgg gtaagaccct gtgaattatg gagtagtcgg
     1141 aatcgtaaat ggtgagagcc acccaatcgc caacagcagg aaagtcttcc cgacttctgg
     1201 ctgagtaacg caggttcccg gttatctcgg cttccaattc acccttttca gttcttacaa
     1261 tgtatctttc tttatgttca gaaataaccc tcccaatctc aaagtcactg aggttattct
     1321 ccaacctgaa cttttcaagc tgtccggtat atcccaaatc ttctaaattc atggctgatg
     1381 attgatgtga gcgaatgtct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 ntcataactc agctttcaat tacagccttg ctttgatagc ttttgtttct tcttcgtagc
     1561 ccggtttttc gagcagtgca aacatgtttt tcttgtaggc ctccacaccg ggttggtcga
     1621 aaggattgac tccaagcagg taaccgctga taccgcaggc tctttcgaag aaatagataa
     1681 gttcgcccag gtagaattca ttcagtttcg gaagttcaat tttcaaattc ggaacaccac
     1741 catccacgtg cgcgatcatg gtgccaagtt cagccatttt atttacctcg tccacccgtt
     1801 tacctgccag gaagttcagc ccgtcgagat tctcatcgga atgagggaat attttcgtgt
     1861 ggttgggctc acagacagaa atcactgttt cgaagatggt acgttcgcct tcctgtatcc
     1921 attgtcccat ggagtgcaga tcggtagtga aatcaacgga tgccgggaag atacccttgt
     1981 tttccttgcc ttcgctttct ccgtaaagct gtttccacca ttcggacaca tagtgcagtt
     2041 taggatggaa gttcaccagt atttcggttt ttttaccgtc acgatagagt tcgttgcgca
     2101 cggctgcata ctgagtcgca ggattttctt cgaaagctgt tcctgttccg caaacttttt
     2161 gcatggttac agctccctgt accagctgac ggatatcaag tccggcgact gcaatgggaa
     2221 gtaatcctac gggtgaaagt accgaatagc gaccgccgat attgtcttcg aggatgaatg
     2281 ttttcagccc ttcctggttg gccagtacac gcaatgcacc ttttgccttg tcggtgatgc
     2341 acacgataag atttttagct tcctcttttc cctgttgttc ttccaactgt gttttcagca
     2401 ggcggaatgc cagagcgggt tcggttgtag tacccgattt ggaaatggaa atgataccga
     2461 aacgttttgt tttcagcagt tcctggagtt cgtagaggta atcttcgccg atattttgtc
     2521 cggcatatac aatcacaggt gaagtacgtt cggtctgtaa ccagtcgaag ctgttggaga
     2581 gcgcgtcgat tactgctttt gctcccaggt agcttccgcc gatgccgatt actaccacta
     2641 cttcgcagtt gtctcgaaga attttggctg tattttcaac gtcggtcagg tgtgcttcgt
     2701 caatttcgga gggcagattg agccatccga ggaagtcgct gccttttccg gttccgtttt
     2761 ggagtgccag gttggcagct tcagcttctg ctttgtaggc agccactttt tcggccgata
     2821 caaatccata ggctttgtcg atagataatt taatgtgttc catattgagt aatgtttatt
     2881 gtaatttctc tgttagttgt tgaatttcca ggataggcga actgcggtta tacagaattt
     2941 cgaatacagc atccactatc gggatgttta cgtgatattt ttcattcagt tcgtgaatgg
     3001 ttttagtggc ataatagcct tcggcaatca tttccatttc gagttgcgcg gttttcaccg
     3061 aataaccttt tccgatcatt gttccgaaga gacgatttcg gctgaatttc gaatatgccg
     3121 ttacgagcaa atcgccgagg aaagccgatt cgctgatgtc ccgatgttgc ggattggtgg
     3181 cgttgcagaa gcgattcatt tcctgcacag cattggatat gagtaccgcc tgaaagttat
     3241 cgccatactt cagtccgctg catatgcccg aggcaatggc gtaaatattc ttcattaccg
     3301 aggtgtactc caatccgtaa acatcgtcgc tgatgcgggc gtgtatgtag tcattggtga
     3361 atttctcggc aatgtaccgg gcattatcgc gcgatttaca ggcaatggtg aggtaggaaa
     3421 gacgttccag cgccacttct tctgcgtgac agggaccacc cagcgccact atattttctt
     3481 ccggaacttt atacttttcg tgaaagtatt cggatataag catattttca tcgggaacaa
     3541 ttcctttgat agctgataca attattttgt catccaggtg gcgtttcagt ttggaaagat
     3601 gctgtttcag gaacggcgac ggagttgcaa aaaccagtat gtctgacatt ttcaccactt
     3661 tgttgatgtc ggtggtaaac cggatgcggt cggtattgaa ccgaacaccc gtgaggtatg
     3721 atggatttct gcttgtccgg atgaattcat ctatctgttc ctgccgacgc atgtaccagt
     3781 tgatggattt gatgttggtc aatgctattt tagccagcgc tgttgcccag ctacctcctc
     3841 ccataatagc gattcgcact ttttcaacat gttttttatc gggagcattt gtaagtatat
     3901 ctttcaggga aatattggtg tttccccatt caatactttc tgttatcatt tttcagtatt
     3961 ttatctatga aatgttttct tatcacggga gtaaaactaa aacgcttgat taacagcata
     4021 agttctatta atcaagcgac agtttattat ctgtttaagc taagtttcct gcagccgcaa
     4081 tccatgcctg cacatcttct tttgccggat tttttagttc cagtttgttt ttcttattca
     4141 gcccgcaaat atcctgatga aacttgttgg ggttcagtcc acgaaggtct cttacctttg
     4201 tgatgcctgc ttttttcacc acctcaatcc attcggccgg tattcccagg gctatatatt
     4261 tatcgtcagt atcctgggtt attacttttt ctggtttcat ctgagggaag aacagtactt
     4321 cctgaatggt ttgctgtccg gtcataagca ttaccaggcg gtccatacca atacccatgc
     4381 ctgatgtcgg tggcataccg tattcgagtg aacgaacaaa atcgaggtcg ataaacatgg
     4441 cttcatcgtc gcccttttcg ctcagtttaa gttgttcctg gaagcgatcg agctggtcga
     4501 tggggtcgtt cagttcggaa taagcattcg ccagttcctt accgttaacc ataagttcga
     4561 aacgttcggt gagttccgga ttttcgcggt gacgcttaca aagcgggctc atttcgcgtg
     4621 ggtaatcggt gataaatgtc ggttgtatgt agttcccctc acatttggct ccgaatattt
     4681 catctatcag tttacctttg cccattgtgt cgtccacttc gatatccagt tttttacaaa
     4741 cttcgaaaag ttgctcctca ttcattccat tgatgtcaat accggtaaat tcatggattg
     4801 aatcaatcat ggaaatgcgt ttgtaaggtg ttttgaagtt gatgatgttg tcgccaacct
     4861 taacttcagt cgttccgttc acgtcaagac aaatcttttc gagcatgttt tcggtgaatg
     4921 tcatcatcca gttgtagtct ttgtaggcta catatatttc catggcggtg aattcgggat
     4981 tatgagtacg gtccattcct tcgttgcgga agttctttga gaattcatat accccttcaa
     5041 aaccgcctac gataagacgt ttcagataca attcgtcggc aatgcgtaga taaagtggta
     5101 tatccagcgc gttgtgatgg gtgataaagg gtcttgctgc agctcctccg ggtataggct
     5161 ggaggattgg tgtttccact tcgagataat ccttcgaatt gaaataatcg cgcatggact
     5221 gatatacttt ggtgcgtttg atgaagatat cttttacacc ttcgttaaca accaggtcca
     5281 cgtagcgctg acggtaacgt tgttcaggat cttcaaaggc gtcaaaggca actccgtctt
     5341 tgtattttac aatgggtagt ggtttcagtg atttgctcaa cacggtcatt tcctgtgcat
     5401 gaacgctgat ttcgcccatt tgggtgcgaa atacaaatcc tttaatgccg ataaagtctc
     5461 caatatcaag caactttttg aaaacggtgt tatacatttc gggttgcgct tcggtgctga
     5521 tgtcgtcgcg actaacatat acctgaatac gtcctttgct gtcctgcagt tccacgaacg
     5581 aagctttacc catgattcgg cgactcatga tgcgtcctgc tatgcacacg tttttaccgg
     5641 tgtcttcgtc tttaaactca gcttttatat ctgttgagaa tgcatttgca gggtattctg
     5701 ctgccgggta aggctctata ccaagcttgc gcaattcggt taaactctga cgtctgaact
     5761 gttcctgttc acttaattcg atactcatat tctgttattt tacaatcgtt tattggcatt
     5821 tatatgaact atgccaatta ttttgcaaaa atacaaaaaa aggatgaatt ttaagtcagt
     5881 tgtgtatgaa tagttgtttg atgttgtgtt tagtagttgt aaatcagaaa aatagagatg
     5941 ctttgtcgtt ttttgtggaa aataattgct aattaactat tcccgagttg gtgtttcaat
     6001 agtaacataa ccaatttgtg aagtttgatt aaaaagtcag gcaggaaaaa atacctgcaa
     6061 gtcattgttt atgatttttt ttgttattgc cggattaatt tttgagctag tttattcccg
     6121 ttgtccagtg tgacatgtag aatgtaaata cctcgcggca gttgtctgat gttcagggct
     6181 tttgaattta tattcggttt gatgtccatc atgaccaatc cagcaacgtt gtaaagtttt
     6241 atatcgctga tgttctgatc cgattctata taaagtattt cctgaaccgg attggggaat
     6301 acacgcagaa taaagtccga tttcagagcc gtatcatcag tggaaattcc caccatattg
     6361 gcatccagcc atgcaatccg ggtggctatc cagtttttaa atttggtaat gtcaccctca
     6421 aaagtagttg gctggtcatc tatttcaggc gatccgttac ctattccgag tgtgttgtaa
     6481 atggattggt gacgttcctg tgcttcgttc agtacacttg caacagagtc aatgtagtga
     6541 tacatataat ccaggctaag aatagatgcc cgcaggctga aataccgatc gtgtatggca
     6601 tttgcaaaca ttggatcctg aagcatcctg tattcccacg atggcggtat tggatcggta
     6661 tcttcacact tgttgatatc ataagcccat ccttctcctg tggtgttttt gtatagataa
     6721 cagtcatcca tgtttttcca ggcccagtcg aaatcccaga tgggtcctga atgtaatcga
     6781 cggtctttac tgtctttgtc tttataaaaa tagcggcttt ttttgtagcc gtccacattg
     6841 cgtgaaagtt cctcgagtat ccagtaatcg acaaaggagt tgacatcaat ataagctctg
     6901 tatccggtaa ccggattgtt aaaatcggaa ctataaagtg ctgtttcaac tgcattgaca
     6961 taactggtaa tgtacgtttt ttgttcatca gctatttcgt tggctttcgg ttcgtgatag
     7021 acgaaatata ccagcgctcc gggtctgttt gccggtgaaa agttacttgt ccagtatgta
     7081 cccgtacctt catcgtaatc ggttttgaaa atgtaacctc cggtaaggga gtcgcccgcc
     7141 agatcggtcg attttagttt ggcgatatca acccggttgt catcaatctt tattttttca
     7201 ccaaagagat atagcccgcg atatgccccg ttgatggtca cttcacaatg tttgagccgt
     7261 ggagcatagt gtcccatttc ttcgaaaatt tttcctgcca gtgtgttgcg catatatacc
     7321 ttgtcattcc agtttgaaag cagtacccag tcattttctt ttggcattcc cagcaatttt
     7381 acgtttagat tttctcccag tgaatcgcgg gtttccaatc cgtaaggtat ctgcggaaag
     7441 cctgcagatg tcgccccccg tatttcaata ccaatctttc cgttatacac attcgcagag
     7501 tctgtcagat ggtttcgtgc tgtatcaccg tgatgaatta tcttcatatc agcttcaatt
     7561 ttgggttcat ccacaatagt agctccgttt ttggtgttta taactatcag tggcagatat
     7621 gacgaggtgt aatcaagtgg agaaataatt tcaggctcga tgaaccagtc aggggtgctt
     7681 ctgaatattg tcgtagcatt ttttattcca aatgacagaa aaacattcga cgaaagatct
     7741 gaaggactgg attttgtgta attgtgaact tcaacggcaa gaatatttat gccaggtttg
     7801 atgcattgca gcaatgtttt tttttcaata acgaattgtt cgggcatgcc cgtcccttcg
     7861 ttttgataca tggttgcttc gtggctggca gtggcagaag tggagaagga tgggtaaaca
     7921 ccgacagtcc cgaggtttgc gcgtgcaatt tccacattgt tgatgtaagc tacaaatccg
     7981 tcgtcataat ccatatccag tgctcccaat attatttgtg aggtgtctgg aatgttaaat
     8041 tttattcgca aatataccga tgtgcaggtg ggaatagtag tgccatcgtc attatcggac
     8101 caaccgaaac ctccggaacc cgacagccag ccagtatcgt tataagtgag ggcccgccag
     8161 gaaggattcg tattgatgcc agggttagaa gtaccaacaa agtatttcca tatatcggat
     8221 ttgaagacag cggtttccca atggtgagcc ttttgtgcaa atccgacagt tatacttgta
     8281 agaaaaaaga gtataagagt gaaaatactt ttcctcatta tttcctgatt ctagtgttag
     8341 ttgtaattta ttaataacaa acaaaaaata aaaaatgttt catccgccta aaggagataa
     8401 aaacaggtaa tgaattgtct ttttttattt gtaattttgc atgaataatt caaagtattt
     8461 ttaacataca tacctatatt aatatgtcat cacttgcaga actatttgaa aaacaaactg
     8521 gcaccaaacc ggtatcggaa gtgcagctga ctgtttcagg ctcaaaccgg aagtattaca
     8581 ggcttaaaaa tgatacgatt tcactgattg gagtgatcgg aacttcggtt gaggaaaaca
     8641 gagcttttat tgctatggct cagcatttca gaaaaaaagg acttcctgtg ccagaacttg
     8701 gcgaagttgc agccgacgaa atgatgtatc tgcaggaaga cctgggcgat gccgtattgt
     8761 tcgattattt agtcaacagc cgcaaaacgg gagtattcag ttccgaggaa aaagcgtttc
     8821 tgcacaaaac aatggaaatg ctggcaaagt ttcaggtggt tggagctcag ggtttcgatt
     8881 tttcggtttg ctacccacag ccagaattca atgaacgctc cattttgtgg gatctgaatt
     8941 atttcaaata ttgttttctg aaatcaacag gtctggattt tcaggaaaac aagttggagg
     9001 acgactttga gaaacttgcg aaggaattgc tcaaagccaa aacagagacc tttatgtatc
     9061 gtgattttca gagccggaat gtaatgctga aagataatga gccgtatttt atcgattttc
     9121 agggaggacg aaaaggaccg ctttattacg atgttgcttc attcatctgg caggcaaaag
     9181 ctcagtatgg gccgcagttg cgcgacgaac tggtggatgc atacctccac tcgttgaaaa
     9241 tatatatgga agttgacgaa gacgaatttc ggcgggttct ggatcatttt gtgcttttcc
     9301 gtttgctaca ggtgcttggc gcatatggtt tcaggggcta tttcgaaaag aaaccgcatt
     9361 ttttacaaag cattccttat gcacttgaaa atttacgggc actcatgaag aataatttca
     9421 aagaatatcc ctacctgatg aaagttctca gggaaatgtc ggaactcaag caattcaagg
     9481 atgttcgtct gcaccgtccg ctggtggtga ctgtttacag tttttcttat aaaaaaggta
     9541 ttccacatga tatttcgggt aacggtggcg gatttgtgtt cgactgccgc gcggttaaca
     9601 atcccggcaa gtacgagcgc ttttactcca tgaccggact cgatgaacct gtaatacagt
     9661 ttctggagaa tgatggtgag attctgaaat ttctcaacag tgcttattcg ctggtcgatg
     9721 catcggtcaa acgatataag gaccgcggat ttactaatct gatgatatgc ttcggatgta
     9781 ccggtggtca acaccgttcg gtatattcgg ctcagaaaat ggctgaacat atccatgatc
     9841 aatttggagt cgaagtgaac cttatacacc gcgaacggaa tctggaacaa acgttcgaag
     9901 aaaccaaaga ctgaaaaaga gattttgctg tttcgatgaa aaaatagtaa ttttgcacgt
     9961 taattaaaaa ttaactccat tcagaatgaa tactacaagt cagcaatttg atcaggtaac
    10021 cgctgtctgc cgtgatattt tcactaaaaa gttgaaagat tacggagcat cgtggagaat
    10081 actccgcccg cgttctgtca ccgatcagat tttcatcaaa gccaaccgca tccgcagcat
    10141 cgaaaccaaa ggagttaaca aggtgggaga ggatataggc ggagaattga tagctatcgt
    10201 caattacgga attatcggac tgattcaact cgaactcggt tttgctgaaa aggatgatct
    10261 gagcattgag gatgcgctga aattgtacaa taaatacctg gaacaggcaa aaaatctgat
    10321 ggcagacaaa aatcacgatt acgacgaagc ctggcgcagt atgcggaccg aatcttatac
    10381 agatatgatc ctgatgaaga tttatcgcac gaagcagata gaggaacacg atggtaaaac
    10441 actcatttcc gaaggcattg atgccaacta ttacgatatg atcaattatg ccgtttttgg
    10501 attgataaaa atgaaattta aagattaaac gaacgaaacg gctttaaata aaaaaacatg
    10561 tatcagcaac atcaacgatc ccgttcggtg gcaaaatccg aagtttacac cttaaaaaaa
    10621 acaggaaaaa tactgctctg gttagcccgg atgattgtcg gaattacttt tgttttttcg
    10681 ggctttgtta aagcagtcga tccgcttggc tctgcctata aatttgaaga ttattttact
    10741 gcttttggca gatttttcga acaccttacc cctatagccc ttccggctgc cattgccctc
    10801 tcaactttag aattactgat aggtttttgt ttcttgttaa aaataaaaat gaagggtatg
    10861 tccgttgtgg cattgctctt tatggcggtc atgctgccgc ttacgcttta cattgccctg
    10921 aataatcccg tatccgattg cggttgtttt ggcgatgcaa tcattctcga caactggaca
    10981 acgtttttta aaaatatcat tctatccctt ttaatagtag ttgtgatgat atttgtaaaa
    11041 caacagcaac caattttctt accagcagca gaatggattg taattacatt agtagcgatt
    11101 gcaggcatag gtttttccgt atataattat cgtcatcagc ctgtcattga ttttcgtccg
    11161 tataagattg gagtgaatat tcctgaaaaa atggtgatac ccgaaggagc tgaaccggat
    11221 aaatatgaaa caatgttggt ttacgaaaaa gacggacaac agaaggaatt taccattgag
    11281 aattatccga aggacagcac ctggacattt gtggaacaaa aaactactct tgtgtcgaaa
    11341 ggttatactc cgccaatcca caatttccag atagtggatc agaatttgaa tgacatcacc
    11401 gattatgtac tgagctacaa agggaccgtt aatctgctgt gtatgtacga cctgaacaaa
    11461 acctctgaaa agggagtaaa agctgccgaa gtattttatc agaaaatcaa atcaaaaggg
    11521 actcctttct acgcacttac tgcttcttcc gacgaggaaa tcgaggcttt ccgcaaccgc
    11581 accggagcta catatgattt ctgcaagact gatcccatta cactgaaaac cattgttcgc
    11641 ggaaatccgg cactgatgat cattaaaaat ggtactgtag ttggcaaatg gaactggaga
    11701 gacttttaat ccatttagaa tgtgttttag cttataatag aagaaataga attaataatt
    11761 aaaccagcct tgccctcagt aaagggtaag ggatatatta aaatgtatga gaaaaaatat
    11821 cgtagcaggt aactggaaaa tgaacaaaac cctgcaagaa ggtgtagctt tggctgcaga
    11881 attaaaagat gtgttggctg gaaaaactcc caaatgtgaa gtagttattg gtacaccgtt
    11941 tattcacctg gctactgtgg caaatcaggt agagggaagt gtaataaaag tagctgccca
    12001 gaattgtgca aataaagaat cgggagccta tacaggcgaa gttagtgcca ccatggtgaa
    12061 atccaccgga gccacttacg taattctcgg tcactctgaa cgtcgtgaat actatggaga
    12121 aacaagtgct atcctgaacg ataaagtagc ccttgctttg tcaaacggac taacacctgt
    12181 ttattgctgt ggtgaagcac ttgaagtgcg caacgccaat acccagaatg aatttgtgaa
    12241 aaatcagctc gaagaaacca ttttcaatct gagcgctgaa gattttcaga aactggtaat
    12301 tgcttatgaa cccatatggg ctatcggaac aggagtaaca gcatcgaccg agcaggctca
    12361 ggaaatgcta gcatttattc gttcactcat tgctgataaa ttcggaaagg aaattgcaga
    12421 gaacacttca atcctttacg gtggcagctg taatgccaaa aatgctcccg aattgttcgc
    12481 tcagcccgac attgatggtg ggctgattgg tggcgcttcg ctcaaagcag cagactttgt
    12541 tgcaattatc gatgcatttt aaatacaaat acatgattgg ataaataaaa atcctgcaaa
    12601 ctgttgtttg caggattttt ttgtattttt gtagtccggt tcgaaataat taaatccatg
    12661 ctgataaaaa tctacaacga taatccaaat cccaatcaga ttcgtcacgt tgctgaggtg
    12721 cttcgtaatg gtggtattat aattgtgccg accgatacag tatatgcttt tggatgcgat
    12781 attttcaatt cggcagcggt ggaatttatc tcaaagctga aaaacaagga tttacgaaaa
    12841 tctgaacttt catttatttg ccacgaaata agccagataa gcgaatatgc caagttggac
    12901 gacgactcct tcaaactgat taaaaaaaat ctgcccggac cttttacttt tatactgaac
    12961 ggaagcagca ggctaccaaa acttttcaaa aataaaaaga cagtaggcat ccgtatgcca
    13021 gataataaaa tcaccctgca gattgtgagg gaattgggta atcccatgat gaccagctct
    13081 atttttgttg acgacgcaac caccgaatac atcaccgatc cggaacttat cgacgaaaaa
    13141 tatggtcatc aggtcgatct cgtggtggat ggcggtatgg gcggactgct ccattcaacc
    13201 gttgttgatt gtaccggcga tcaggcagaa atcatacgcg aaggatttgg cgaattgcag
    13261 taatctgcgc cagcagagag atactgggca ttttcttcgc tttcatacgt acaatatctg
    13321 ataaacagat cactcccaga aaaaatcttt tcaaattatt ttgccaatca aaacaaaaac
    13381 agtatttttg cagtccgttt attatcccgg taaaatagtc tgtttccgac gagttaaata
    13441 tcctattatg tgccgattag cctctctcat aatagccgat gtgtcggtgg aaaagactga
    13501 atattaatta taaaaacaat ttataaaagt ggatacgtta agttataaaa ccatttctgc
    13561 caataaagcc accgtgcaga aagaatgggt agtagttgat gctactgata tggtgttggg
    13621 acgtatgggc tcgaaagttg caaagctttt gcgcggtaaa tacaaaccaa gttttactcc
    13681 tcacgttgat tgcggagaca atgtaatcat aatcaatgcc gataaagtta aattaaccgg
    13741 taacaaatgg actgacagag tttaccttcg tcacaccgga tttcccggag gacaaagaga
    13801 atacactcct tcagatttga tggctaaaga tccggatcgt ctgataagaa aagtggtaag
    13861 aggtatgttg ccaaaaaacc gtcttggtga taaaattatc aacaatctat atgtgtttgc
    13921 aggtgcagaa cataatatgc aagctcagaa accgaaacaa attgatttaa actcacttaa
    13981 ataaccgata tggaagtaat aaatgcatta ggaagaagaa aagcagctat tgcccgcgtt
    14041 ttcgtaagtg aaggaaaagg acaaattact atcaataaaa gagatcttgc cgtttatttt
    14101 ccatcaccaa tactgcaata tgttgtaaaa caaccgctga ataaattagg tgttgcagaa
    14161 aaatatgata ttaaagtcaa tctggacggt ggtggctaca aaggtcaggc tgaagcatta
    14221 cgtctggcta ttgcacgtgc attggtgaaa attgatccgg aatgtaaaaa atctctcaaa
    14281 gtagaaggat ttattacccg tgatccacgc gaagtggaac gtaaaaaacc gggacgtcca
    14341 aaagcacgca agagattcca attctcaaaa cgttaatatc ttacaaagca gtctgtttct
    14401 ggctgcttgc tgaatatcgt ttagtatcta aattgttgag atcttttatc gtttacctgt
    14461 tttttatcgg tcaactggaa agctactcaa caattggttc taataaaatc agaattaaaa
    14521 aagaaagtaa acaaaataat aaaacaaaaa aatgtcaaga acaaatttcg aagaattatt
    14581 agaagccggt tgtcattttg gccacctcaa acgcaaatgg aaccctgcaa tggctccata
    14641 catctttatg gaacgtaatg atattcatat cattgacttg cacaaaaccg tagtaaaaat
    14701 agacgaagct gcagcagcta tgaaacaaat agctaaatca ggccgcaaaa tcttatttgt
    14761 tgctacaaaa aaacaagcta aagatgtggt tgccgataaa gcaacatcag ttggaatgcc
    14821 ttacattaca gaacgctggg ctggtggtat gttgaccaac ttcccgacaa tccgtaaagc
    14881 tgttaagaaa atgacaacaa ttgacaaaat gtcgaccgat ggtacttttg ataatatctc
    14941 caaacgcgaa aaacttcaga ttacacgtca gcgtgaaaaa ctggaaaaga accttggttc
    15001 tatcgccgat cttacccgtc tgccttctgc acttttcgtt gtcgacgtaa tgaaagaaca
    15061 catcgcagtg aaagaagcac aacgtctggg tattcctgtt ttcggaattg ttgatactaa
    15121 ctccaatcct aatgatatcg atttcgttat tcccgctaat gatgatgcta caaagtctgt
    15181 tgaagtaatc ctcaacgcta tgtgcgcagc tattcaggaa ggtctcgaag aacgtaagat
    15241 tgaaaagatt gatgctgaag cagctgaagc tcctgaagtt aaggaaaaga aatcacgtgt
    15301 ttcgaaaaga cccagaactc agaaagaaga tgacgaagct ctgaaagcta acgttgttaa
    15361 gaaattcgtt aaggaagaag aataaaatac cccggactct taaaggggaa accgggattt
    15421 aagaataagg ttacgggtta taatttattt gtagcttgta accttttttt caaaaatcaa
    15481 actaaataat tattacaaaa taacaacgac tttccctaaa cccctccatg gggctgggag
    15541 tcataaaata ttacaattat ggcagtaaca atggcagaaa tttcaaaatt gcgcaccatg
    15601 acaggtgccg gtatgatgga ctgtaaaaat gcactcgtcg aagctgaaaa cgattttgaa
    15661 aaagcaatag aaattatacg taaaaaagga aaagcgattg ctgcaaaacg cagcgaacgc
    15721 gaagcatcgg aaggttgcgt actggcagcg gcaaaagatg gttttgcagc tgtattggct
    15781 ttgaagtgtg aaacagactt tgttgcaaaa aatgctgatt ttatagcatt aacacaaaca
    15841 atacttgata aagcattagc agaaaaacct gaaagtatag atgcacttaa ggctttgact
    15901 attacagaag acggacgtac aatcgccgat cttgtaaccg aacgctcggg agttaccggt
    15961 gaaaaaatgg aagtcgatta ttatgaattt gtaaaaggcg gcacggtaac atcttacatt
    16021 catccgggta acaagttagc tactatcgtt gcctttgctg aaaatgacgc agaatacgaa
    16081 accatacatg gtgtagcaat gcacattgcc gcaatgtcgc ctgtggctat tgatgaatcc
    16141 gccgtacctc aaaaggtaaa agataacgag ctggaagtgg ctatcgaaaa aactaaaatc
    16201 gaacttgttt ctaaggaagt tgaagtcgct ctgaaaaaag caggcatcaa ccccgcccac
    16261 gttgatagcg aagaccatat tgaatcaaat atggcaaaag gctggatcac agcagaagat
    16321 gcagcaaaag cccgcgaaat aaagacaact gtctttgcag aaaaatcagc aactctaccc
    16381 gaacagaaag taaacagtat tgcagcggga cgtatggcta aattctacaa agaatatacc
    16441 cttgtagaac aaaagtacga aggtggtggc gatgaagccg gaaaaacaac tgtaaaagaa
    16501 ctgcttgcaa aaaaacattt aacctgcatc gcttttaaac gagttacatt aaatcaggaa
    16561 taatcgttta agtataatta caaaacaata aacctctcga aattccgaga ggttttttgt
    16621 tatatttcac ttttaatcgt aaacctttga accgttagaa atatttgaat tttttaatct
    16681 tttgaatttc ataaactttt ctgtaatttt gtatcttcat cattaatata taaaatggat
    16741 aaaaaagtca gagtgcgttt tgcaccaagt ccaaccggac cgctacatat cggcggggta
    16801 cgtaccgcat tatacaatta tttatttgca cgtcatttcg gaggtgatat gcttctgcgt
    16861 attgaggata cagattccgc ccgatttgtg ccgggagccg aagaatacat cgttgaagca
    16921 cttcagtggc ttggtataac catcgatgaa ggaatcggag cacagactgc cggaaaacac
    16981 gcgccatacc gccagagcga aagaaaagaa atctacaaaa aatatgttga tcaattgttg
    17041 gctgagggaa aagcttatct ggcttttgat acacctcagg aacttgattt ccagcgtgga
    17101 aaaataaata attttcaata cgatgccacc acacgcggat tcatgcaaaa ttcgctcacg
    17161 ctgacaaagg aagaagttaa aaaaagactg gctgatggag agcagtatgt ggtaagaata
    17221 aaaattgaac caaatattg
//
LOCUS       DAOPYB010000050        17071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3545_length_17071_cov_10.637870, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000050 DAOPYB010000000
VERSION     DAOPYB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17071
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3545_length_17071_cov_10.637870"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PKH58_05625"
     CDS             complement(<1..433)
                     /locus_tag="PKH58_05625"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNX88539.1"
                     /translation="MVSYKELGLVNSKELFKKAIEGKYAIPAFNFNNMEQMQAIIAAC
                     VETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGYAIPIVLHLDHGDSFELCK
                     SCIDYGFSSVMIDGSHLPYEENIALTKKVVEYAHAHDVTVEG"
     gene            complement(556..2352)
                     /locus_tag="PKH58_05630"
     CDS             complement(556..2352)
                     /locus_tag="PKH58_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNX88540.1"
                     /translation="MNPIQLSRLIGTQAKKYGEKTVLYDRAEITEPWKAISWNEMSSK
                     VDIAAKSFLELGVKDQQRVAQFSQNKAENLIIDFALFSLRAVMVPIYPTSTVQQVEFI
                     VNDAEIEYMFIGDQKQYDVAVEILKTSGTLKMLIAFDKDVEFSVPDKSMYYDTFLAIG
                     EKSTRMDEVEKLRDQLSEKDYANILYTSGTTGNPKGVIMTHEMFNEGMRIHTIRLKSM
                     NDQDISLAFLPLTHVFERTWCYFLLYSGATNYINHRPADIQQTIKDVHPTLMCAVPRF
                     WEKVYIGVQEVLNNYSPFMLGVVTWAIAVGKKHNIDRLRIGKKPDAWLSLRYKIADSL
                     VFSKVKKTLGLENAKILPVAGAKLSDDIAMFFRSIGVPIVYGYGLTESTATVSCFEEQ
                     YQIGTVGSIMPDVQVKVGTDNEILLKGKTITPGYYNNPEANKKAFTEDGWFKTGDAGK
                     IENNRIILTERLKDLFKTSNGKYIAPQEIETRLGTDKYIEQVAVIGDERNYVTAIIAP
                     SIPALEAFAKEHNIAYKDKDELLLLPEIYSLMEQRIAAAQKGMAPYEIIKKFTLIKIP
                     FTIEAGELTNTLKIRRAVVQQKYKAQIDAMYK"
     gene            complement(2457..3224)
                     /gene="rnc"
                     /locus_tag="PKH58_05635"
     CDS             complement(2457..3224)
                     /gene="rnc"
                     /locus_tag="PKH58_05635"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNX88541.1"
                     /translation="MKRLFSNKRKEPFLSFFKVLGFYPRNIEYYQLAVRHKSISIQDP
                     KGRLLNNERLEFLGDAVLNSIISDILYHRYPDENEGFLTNARSNLVKRDSLNELCVRT
                     GLDQLLITIKHLNPDKSNNIYGNALEALIGAIYLDYGYEKCGEFIRQRLFVSFDILKK
                     TAEGDENYKSKLLEWTQKNRLEAEFRIDADTVDAYNQHTFRTQLLIEGIVVSEGVGAS
                     KKESQQHASAAAFQLINNSPGFVDKITDKANHNVTTN"
     gene            complement(3247..4509)
                     /gene="fabF"
                     /locus_tag="PKH58_05640"
     CDS             complement(3247..4509)
                     /gene="fabF"
                     /locus_tag="PKH58_05640"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNX88542.1"
                     /translation="MELKRVVVTGVGAVTPLGLTAAETWQNVLSGKSGAAPITYFDPS
                     KHKTRFACEVKGFDPQKYFDRKEARKMDLYTQYALVATAEAIENSGIDLEAIDKDEVG
                     VVFAAGIGGISTFEQEVGYYYSNLEQGPKFNPFFIPKMISDIAAGQISIQYGFHGPNF
                     STASACASSNNAIIDAFNLIRLGKANIIFAGGAEAAITPAGVGGFNALTALSTRNDDP
                     EGASRPFSLSRDGFVMGEGAGCLMLEELEHAKARGANILCEIAGTGLSADAYHLTATH
                     PEGLGAMLVMKRALAEAGMKPEDIDYINVHGTSTPVGDISESKAIKDVFGEYAYKLNI
                     SSTKSMTGHLLGAAGAVEAVISIFSILNNVVPPTINFNEGDEDPEIDYKLNFTFNKAQ
                     QRTVDAALSNTFGFGGHNASVIFKRYEK"
     gene            complement(4534..4770)
                     /locus_tag="PKH58_05645"
     CDS             complement(4534..4770)
                     /locus_tag="PKH58_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNX88543.1"
                     /translation="MSEVAEKVKAIIVDKLGVDEADVVETASFTNDLGADSLDTVELI
                     MEFEKEFGVSIPDEEAQKITTVGDAIAHVEALKK"
     gene            4921..5487
                     /locus_tag="PKH58_05650"
     CDS             4921..5487
                     /locus_tag="PKH58_05650"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNX88544.1"
                     /translation="MPKNIAIFASGSGSNAENIIRYFTGSAELKFPVILSNKPDAFIH
                     ERAKNLNVPSLTFSKQEFEAGDNILEVLKSYDIYAIVLAGFLLKVPVQIIKAYPHRII
                     NIHPALLPKYGGKGMYGERVHKAVKEQGDTESGITIHYVNENYDEGNVIFQALCKVLP
                     TDTCEDIAAKVHALEYEHFPKVIEQIWG"
     gene            5600..6703
                     /gene="ychF"
                     /locus_tag="PKH58_05655"
     CDS             5600..6703
                     /gene="ychF"
                     /locus_tag="PKH58_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNX88545.1"
                     /translation="MSLQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVI
                     TVPDERLNKLAELVNPKKIIPTTVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     VLRCFDDHNVTHVDGTVDPVRDKEIIDAELQIKDLETIEARINKVEKQAKTGGDKEAL
                     RAFHIFSQIREALMKGNSARTVTFDNKDDAKLAKDLYLLTSKPIMYVCNVDEASAVKG
                     NKYVDAVREATRDENAEILVVAAKIESEIAEFETYEERQMFLEEIGLQESGVNRLIKA
                     AYHLLQLETFLTAGPDEVRAWTYHKGWKAPQCAGVIHTDFEKGFIRAEVIKYSDFIAL
                     KSEAACKEAGKMNVEGKEYVVQDGDIMHFRFNV"
     gene            6755..7291
                     /locus_tag="PKH58_05660"
     CDS             6755..7291
                     /locus_tag="PKH58_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX88546.1"
                     /translation="MKVADNPATFFIFEKFKNAMLIIRPIQQSDNKSLATIIRRCFYD
                     FGAPTAGTVYEDPTTDDLFSLFTKEKSVLWVAESDGKVLGCCGLFPDDKLPKGTVELV
                     KFYLAASARGKGIGKALMERTIGSAREFSYQSIYIESLPEFSIAVSLYEKYGFRYLKK
                     SLGKSTHPGCNVWMLKDL"
     gene            complement(7353..8522)
                     /locus_tag="PKH58_05665"
     CDS             complement(7353..8522)
                     /locus_tag="PKH58_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNX88547.1"
                     /translation="MKRLLIYSLMLCMTASLAAQNYNDTTFNFTVVKENKITPVKNQA
                     NSSTCWSFSGLAFFESELLRLGKPAVDLSEMFVVHHSYSDKADKYLRMNGTSNFAPGG
                     SFYDDVYVWKTYGMIPDSVMNGLNYGETKHNHGELDALTGAFMTTMVKNPNKKISSAW
                     KPAFEGILNAYLGKLPTEFTVNGKKYTPVSYANSLGLNMDDYVSITSFTHHPFYTKFA
                     IEIPDNWRWAESYNIPLDEFDRIFDYAVENGYTVAWGSDVSEKGFTRNGIGLLPETKA
                     ENLNGTDQAKWVGKQKDMKYDIKGPMIEEKVTQESRQKGFDNYQTTDDHGMLIYGIAK
                     DQNGTPYYMVKNSWGTDNKYKGTWYVSKPFVMSKTINILVHKDAVPKDIRKKLGI"
     gene            complement(8610..10073)
                     /locus_tag="PKH58_05670"
     CDS             complement(8610..10073)
                     /locus_tag="PKH58_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNX88548.1"
                     /translation="MNSNDIRNLEPKALWENFHGITQVPRPSGKKEQIGKYLEDFGKS
                     LGLETLRDEIGNVLIRKPATPGMEDRKGVILQAHMDMVPQKNSHVNHDFEKDPIQTHI
                     DGEWVTANETTLGADNGIGVAAAMAIMQSTGVAHPAIEMFITVDEETGMFGAFGLQPD
                     FLKGDILINMDSEDEGELYVGCAGGIDADITFKFKEVEVPEGDVAFKLSLTGLKGGHS
                     GVDIHLQRANANKLMFRFLKEAVADYDARLCCIEGGTLRNAIPREAFAAITVPQEGVD
                     ELMELVGEYEALFRTEFEGVEDGISFTAERTDKVKGLMPEEVQDDLINAITACPNGVY
                     RFIPEVPDVVETSNNLAIVRSEGNTIGVSCLIRSSVESRKEELASMVESVFALAGAKV
                     EFKGGYPGWKPNLDSPILKEMTNIYEKNFGKTPRIMIIHAGLECGILGRNYPYMDMIS
                     FGPTIRFPHSPAEKVNIPTVQKFWDFLLLTLANIPKK"
     gene            10320..11825
                     /locus_tag="PKH58_05675"
     CDS             10320..11825
                     /locus_tag="PKH58_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HNX88549.1"
                     /translation="MQNNFRFMSADEAAAFINNGDIVGFSGFTAAGCPKVVPTAIANR
                     ATAFHNEGKEFKIGMYTGASTGDSLDGALARAKAVNFRTPYQSNKDMRNALNSGEVAY
                     YDLHLSALALSLRYGFIAPPKFAILEICDLTPEGEVVLTAGVGISPTIVNLAEHIILE
                     WNHYHPKELKGFHDIYEASNPPSRREIPIYTPSDRIGTTTLKLDPKKILCVVDTNQMD
                     EVGGFTPVDEVTARIGENVATFLASELKQNRIPKEFLPLQSGVGNIANAVLASLGANK
                     DIPAFEMYTEVIQDSVIDLMKQGRVKFASGCSLTVSPDMLKEVYDNLDFFRDKIVLRP
                     QEISNSPEIARRLGLISINTALEADIFGNVNSTHVLGSKMMNGIGGSGDFTRNAYFSI
                     FTTPSTAKGGKISAIVPMVSHNDHSEHSVKVLITEHGIADLRGKSPIQRAHLIIENCV
                     DPAYRQLMYDYLKLGEKNGHTPVNLAAGFGMHLQFAKSGNMLDTKWEDYTE"
     gene            11917..13584
                     /locus_tag="PKH58_05680"
     CDS             11917..13584
                     /locus_tag="PKH58_05680"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNX88550.1"
                     /translation="MKSDIEIAHEIELERIKNVAHKMGIHRESIISFGPYIAKIPISV
                     IDSKKIKKSKLILVTAITPTKAGIGKTTVSIGLSLGLNKIGKVAAVALREPSLGPCFG
                     MKGGAAGGGYAQVLPMENINLHFTGDFHAITSANNMIAALLDNYMYHHQHQADGIKQV
                     LWKRVLDVNDRSLRNVVTGLGGLTNGIPAETGFDITPASEIMAILCLSENEDDLKRRL
                     EKILLGYTYSGKPFTVKDLGVAGSIAVLLKDAMHPNLVQTTEHTPAFVHGGPFANIAH
                     GCNSVVATKMAMNFCEYVVTEAGFGADLGAEKFMNIKCRKAGIQPVLSVLVVTTQALK
                     MHGGVQVKDIKLPNIEGLQRGFENMDKHIQNLHSFGLQVLVSFNKYDFDSEEEIELLS
                     NHCKEMNVGFAVNDAFTKGGEGAVELASKVVEMTSGKCEKTIHYTYKDTDSIQRKVEK
                     IAMKLYGARRVIFSEKALKMIPKILEMGGAEYPVCIAKTQYSFSDDERLYGVAKDFDF
                     HVNELVLNNGAEFIVAIAGSIMRMPGLPKEPQAANIDLIDGEIVGLS"
     gene            complement(13751..14656)
                     /locus_tag="PKH58_05685"
     CDS             complement(13751..14656)
                     /locus_tag="PKH58_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNX88551.1"
                     /translation="MIPDFQTIMLPFLKIISDGEEHTTIETNQKLATHFNLTDKELDE
                     YLPSGSQKTFPNRVAWTKSHLKMAGLLENTKRSSFRITEAGKQLLNTNPIEINLRVLK
                     TIPAYQERTGRTHDEDSSADPENSQISATPEEIIENSYLTIRKSLAQELLFKIKSSTP
                     SFFETLVVELLVKMGYGGSIKDAGRSIGRSGDEGIDGIIKEDKLGLDVIYVQAKRWEN
                     VVGRPEIQKFVGALAGQGAKKGVFITTSRYTNDARDYQPRNETKIVLIDGEQLAELMI
                     DHNLAVSTVNTFEIKRIDNDYFGDE"
     gene            15036..16406
                     /locus_tag="PKH58_05690"
     CDS             15036..16406
                     /locus_tag="PKH58_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX88552.1"
                     /translation="MKNAKLLILLSFVLCFSGISAQESPSKEYQDNYKKVMDRTAWWR
                     NDRFGMFIHFGAYAVAARGEWVKSNEEITTESYQKYIDEFNPVDFDARKWAKIAKAAG
                     MKYAVLTAKHHDGYCMFDSKLTDYKLSTRFGGRDLVREFLEAFRAEGIKVGLYYSIID
                     WHHPDYPNVGNHPQRNDKEYGKRKFNWDNYLKYMHGQVEELVRDYGKIDIMWFDYSFD
                     EYSGEKWKAKELVQMVRKYQPDIILDNRLKHNDGISSKQRTISTLGDFETPEQGIPDV
                     PLTDKFGNAIPWETCLTLNGVWGYNESDKNWKSPELIVHTLVNCVSKNGNLLLNVGPD
                     ARGNIPQESVSILKEVGNWMAKNSESIYGCGSSALAKPDWGRYTSKGNTLYAHWLYPN
                     IGDINTRGVDGDKVKAVYMLCTGAELPYQKSWWGNTEPGNLFISVGRNPQKPYKYDTV
                     LKIQFK"
     gene            complement(16826..>17071)
                     /locus_tag="PKH58_05695"
     CDS             complement(16826..>17071)
                     /locus_tag="PKH58_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88553.1"
                     /translation="KAENLFITKYFKLVGLISVTRRTFRSSRASATKRKEVLKNISFF
                     CFLYFQEGGSVQPEIPGRFFSTAESLIISADHFLLNR"
BASE COUNT         4798 a         3422 c         3597 g         5254 t
ORIGIN      
        1 caccttctac agttacgtcg tgagcatgag cgtattcaac tactttttta gtcaaagcga
       61 tgttttcttc gtaaggcagg tgtgaaccat caatcattac tgaagaaaag ccatagtcga
      121 tacagctttt acacaattca aagctgtcgc catgatccag gtgtagaaca ataggaattg
      181 catatccgag ttctttagca tattctacag caccctgagc catgtagcgg agcaatgttt
      241 ggtttgcata tttgcgtgcg ccgctcgaaa cctgaagaat tacaggtgat tttgtttcta
      301 cacatgcagc aataattgcc tgcatctgtt ccatattatt gaagttgaaa gcaggaattg
      361 catactttcc ttcaattgct tttttgaata actctttgga gttaaccagt ccgagttctt
      421 tgtaacttac catattgatt attttttatt tagtttgttt cgtgattaaa ttattttgca
      481 aaaatactat ttttttgtaa tgtgtacaag tgaaacttaa gttgttatat gagcagatag
      541 accttgatca atctcttatt tatacattgc gtcaatttgt gccttgtatt tctgttgtac
      601 tactgcacgg cgtattttca gcgtgttggt cagttcgcct gcttcgatgg taaagggaat
      661 tttaatcaga gtgaattttt ttattatttc gtatggtgcc attcccttct gggcagcagc
      721 aattctttgt tccatcagcg aatagatttc aggcaacagc aacaactcat ccttgtcttt
      781 ataagcgatg ttgtgttctt ttgcaaaggc ttcgagagcc ggaattgaag gtgctatgat
      841 ggctgtcaca tagttgcgct cgtctccgat gactgcaacc tgctcaatat atttatcggt
      901 gcccaatcgg gtctctattt cctggggagc aatgtatttt ccgttcgatg ttttaaataa
      961 atctttcaaa cgttcggtaa gtattatcct gttgttttca atttttccgg catcaccggt
     1021 cttaaaccat ccgtcttcgg taaatgcttt tttatttgct tccggattgt tgtagtaacc
     1081 gggggtaatg gttttacctt tcagtagaat ttcgttatca gtccctactt taacctgaac
     1141 atcgggcata attgagccta cagtcccgat ttgatattgt tcttcgaaac aacttaccgt
     1201 agctgtactt tctgtcagac cataaccgta aacaatcgga actccaatgc ttcggaaaaa
     1261 catggcaata tcgtctgaaa gttttgcgcc tgcaaccgga agtatttttg cgttttcaag
     1321 cccaagagtt ttttttactt tcgaaaatac cagtgagtcg gctattttat atcggagact
     1381 taaccaggcg tccggctttt tcccgattcg taaacggtca atattgtgtt tcttccctac
     1441 ggcaatggcc caggtaacaa ctccaagcat gaacggagaa tagttgttta gtacttcctg
     1501 aacaccgata tatactttct cccagaaacg tggtacagcg cacatcagtg tcggatgaac
     1561 atcctttatg gtttgctgaa tatcggcagg acggtgattg atgtagttag tggctccgct
     1621 gtagagcaga aagtagcacc aggtgcgctc gaaaacgtgt gtaagaggaa gaaatgctaa
     1681 tgagatatcc tgatcgttca tcgatttcag tcgtatggta tgaatacgca tcccttcatt
     1741 gaacatctcg tgggtcataa ttactccttt aggattgccg gtggttcccg atgtgtacag
     1801 aatgttggca taatcttttt cacttagctg atcgcggagt ttctccacct catccatacg
     1861 tgtcgatttc tcgccgatgg ccagaaatgt gtcgtaatac atcgatttgt cgggaactga
     1921 gaactctaca tctttatcaa aggcaataag catcttcaac gtccctgaag ttttgagtat
     1981 ttctacagca acatcatatt gtttttggtc gccgataaac atgtattcaa tttctgcatc
     2041 atttacaata aattcaactt gttgtactgt cgaagtgggg tagatgggaa ccataacggc
     2101 acgcagtgaa aaaagcgcga aatctatgat cagattttca gctttgtttt gcgaaaactg
     2161 agcaacacgt tgctgatctt ttactccaag ttcgagaaaa ctctttgctg ctatatcaac
     2221 tttcgagctc atctcgttcc agctgattgc tttccacggt tctgtaattt ctgctctgtc
     2281 gtataagact gttttctctc cgtatttttt tgcttgtgtg cctatcaggc gtgataattg
     2341 tattggattc attgttgatg tattttgccg gatcttgttt ttatgaaagg taatacccaa
     2401 aaaataccgg actttatgaa taaacacatt gtttgttaat aatgttctga ttaaaattaa
     2461 tttgtcgtca cattatggtt tgctttgtcc gttattttgt ccacaaaacc ggggctattg
     2521 ttgatgagtt gaaaggccgc tgcagaagca tgttgttgtg attctttctt gctcgcaccc
     2581 acaccctcgc ttaccacaat tccttcaatc aataattggg tgcgaaaagt atgttggtta
     2641 tatgcgtcaa ctgtatcagc gtcaattctg aattctgcct ccagtctgtt tttttgtgtc
     2701 cattcgagca gttttgattt gtaattttca tctccttccg cagttttttt cagtatgtcg
     2761 aacgaaacga aaagtcgttg gcggataaat tccccgcatt tctcataacc ataatcaaga
     2821 taaatggcgc caatcaacgc ttcgagcgcg tttccataga tgttattgct tttatccgga
     2881 tttaaatgct tgatggttat caggagctgg tcaaggccgg tgcggacaca taactcatta
     2941 agcgagtctc gttttacaag attcgaacgg gcgttagtca gaaacccttc attttcatca
     3001 gggtagcgat ggtagagtat atccgaaata attgaattta gtacggcatc tcctaaaaat
     3061 tcaagtcttt cgttgttcaa aagtctgccc ttcggatctt gtatagatat tgatttatgt
     3121 ctgacggcaa gctgatagta ttcaatattt cggggataaa atcccaaaac tttgaaaaaa
     3181 gacaaaaaag gctctttccg tttattggaa aagagccttt tcatatttaa gaagttttgt
     3241 ctcaatttat ttttcgtatc ttttgaatat aacacttgca ttatgaccgc caaatccaaa
     3301 agtatttgac aaggcagcat caacagtacg ctgttgtgct ttgttaaaag tgaaattcag
     3361 tttgtaatca atttcaggat cttcatctcc ctcattgaag ttaattgtag gaggaactac
     3421 gttgttcagt atcgaaaaaa ttgagattac agcttccact gctccggcag cacccagcag
     3481 gtgacctgtc atcgattttg tggaactgat attcagttta tatgcatatt ctccaaacac
     3541 atccttgata gcctttgact ccgagatatc tcctactggt gtagaagttc cgtgcacatt
     3601 gatataatca atatcttcag gtttcattcc ggcttctgca agtgctcttt tcattaccaa
     3661 catcgctcca agtccttcag ggtgtgttgc ggtaaggtgg taagcatcag cagataaacc
     3721 cgtgcctgct atttcacaaa gaatatttgc accacgggct ttggcatgtt caagttcctc
     3781 aagcatcagg cagccggcac cttcacccat aacaaatccg tcgcggctca gactgaatgg
     3841 ccttgaagct ccttccggat catcattgcg tgttgacagg gctgtaagcg cgttgaatcc
     3901 gccaactcca gcaggggtaa tggcagcttc agctcctcca gcaaatatta tattggcttt
     3961 tcccaatcgg atcagattga atgcatcaat gatcgcattg ttggaagagg cacacgcaga
     4021 ggctgtcgag aaattaggac cgtgaaaacc atattgaatg gaaatctgtc cggcagctat
     4081 gtccgaaatc attttcggaa tgaaaaaggg attgaatttg ggaccttgct ccagattgga
     4141 ataatagtag ccgacttcct gttcgaaagt actgatacca ccaatacctg cagcaaaaac
     4201 cacacccact tcgtctttgt caatagcttc cagatcaatg ccactgtttt caatggcttc
     4261 agcggttgct accagggcgt actgagtgta caaatccatt tttctcgctt ccttgcggtc
     4321 gaaatatttc tgaggatcaa aaccttttac ctcgcaggca aatctggttt tgtgttttga
     4381 ggggtcaaaa taagtaattg gggccgcgcc gcttttcccg gataagacat tctgccacgt
     4441 ttccgcggca gtaagtccta aaggggtaac ggcaccgacg ccagttacta caactctttt
     4501 taattccata aaattttact tttaggaatt gaattatttt ttcaaagctt ctacgtgagc
     4561 gatagcatcg ccaactgttg taattttttg agcttcttcg tcaggaatag aaacaccgaa
     4621 ttctttttcg aattccatga tcaattcaac tgtgtccaga gaatctgcac caaggtcatt
     4681 ggtaaaactg gctgtttcaa caacatcagc ttcgtcaaca cccaatttat ctacaataat
     4741 agcttttact ttttctgcaa cttctgacat aattttgctt tttagttatt aaataaaatt
     4801 tgttttataa atttgcggtt gcaaagtaaa gaaaatttca ttaaaccacc gcacattttt
     4861 tctaaaaaaa gcaatttatt gcacattttt aagtgaaatg agcataaaat cattccggct
     4921 atgccaaaaa atattgccat atttgcttcg ggttcagggt ctaatgctga aaatattatc
     4981 cgctatttta ccggttctgc tgaactcaaa tttccggtta ttctttccaa caaaccggac
     5041 gcgttcattc acgaaagggc taaaaacctg aatgttccat cactgacttt ctcgaaacag
     5101 gaatttgaag ctggcgataa tatccttgaa gttttgaaat cgtacgatat ttatgctatt
     5161 gttttagccg gctttttgct gaaggttccc gttcaaataa ttaaagcata tccacacaga
     5221 atcatcaata ttcatccggc attactgcct aaatacggtg gcaaaggaat gtacggtgaa
     5281 cgtgttcata aagctgtaaa agaacagggc gatactgaaa gtggtattac aatacattat
     5341 gtaaacgaaa actacgatga aggtaatgtg atttttcagg ctttatgtaa ggttttgcca
     5401 accgatacct gcgaagatat tgctgccaaa gtacatgctc ttgaatacga acatttccct
     5461 aaagtgattg agcagatttg gggatagaca gtttttctag tatctttgca ggcaaatatt
     5521 attaattaca ggcttgctga tgtactggca cataatgcta tcataccata agttaagcga
     5581 actaaacatc actatataaa tgtcattaca atgtggtata gttggtctac ccaacgtagg
     5641 gaaatcaact cttttcaact gtctatctaa tgcaaaggca caggccgcga actttccctt
     5701 ctgcaccatc gaacccaatg tgggcgtcat caccgtgccc gacgaacgcc tgaacaaact
     5761 ggcagaactg gtaaatccga aaaaaattat tccgacaacc gtggaaatag tggatattgc
     5821 cggactggta aaaggtgcaa gcaagggcga agggcttgga aataaatttc ttgccaatat
     5881 ccgcgagacg gatgccattc tacacgtact tcgttgtttc gacgatcaca atgtaacaca
     5941 cgttgacgga acggttgatc cggttcgcga caaggaaatc attgatgccg aactgcagat
     6001 caaagatctt gaaaccatcg aagcacgcat caataaagtg gaaaaacagg caaaaaccgg
     6061 tggagacaaa gaagcattgc gtgcgtttca tattttttct caaattcgcg aagccttgat
     6121 gaaaggcaat tcggcccgaa ctgtaacttt cgacaacaaa gatgacgcaa agctggcaaa
     6181 agacttgtac ctgctgactt ccaaacccat aatgtatgta tgcaatgtgg acgaagccag
     6241 tgcagtgaaa ggtaataaat atgtagatgc cgtgcgtgaa gctacccgtg atgagaatgc
     6301 agaaatactt gttgtggcag caaaaataga atcggaaatt gccgaatttg aaacatacga
     6361 agaacggcag atgtttctgg aagagatcgg attgcaggaa tccggtgtaa accgcctgat
     6421 taaagctgcc taccatttac tccagctgga gacatttctt actgccggtc ccgacgaagt
     6481 tcgtgcctgg acttatcaca aaggttggaa agctcctcaa tgcgcgggag ttattcacac
     6541 agactttgaa aaagggttta tccgtgccga agtaatcaaa tacagtgatt ttattgcgct
     6601 gaaatctgaa gcagcctgca aggaagccgg caaaatgaat gtggaaggca aggaatacgt
     6661 cgttcaggat ggcgatatca tgcattttag attcaatgta taaggctata gcacgtctaa
     6721 aacaaataat acgaactgat tataacaaat ttgaatgaaa gttgccgaca atccggcaac
     6781 tttttttatt tttgaaaaat ttaagaacgc aatgctgatt ataaggccta tacaacaatc
     6841 agataataaa tcgctcgcga ccatcattcg tcggtgtttt tatgatttcg gtgcgcctac
     6901 tgccggaacc gtgtacgaag accccacgac tgatgatttg ttttcacttt tcacgaaaga
     6961 aaaatctgtg ctctgggtgg ccgaatcgga tggaaaagtg ctgggatgtt gcggtttatt
     7021 tcctgatgat aagttgccta aaggaacggt tgaacttgtc aagttttacc ttgctgcttc
     7081 ggcgcgtggc aaaggaattg gtaaagcctt gatggaaaga accatcggtt cagcgcgcga
     7141 atttagctat caaagtattt atatcgaaag tcttcccgaa tttagtattg ctgtttctct
     7201 gtatgaaaaa tacggattcc gatacctcaa aaaatcactc ggcaaatcta ctcatccggg
     7261 ttgcaatgtg tggatgttga aagaccttta atagaaaagg cacagcagtt gaagttttgc
     7321 tgtgcctcgt ataggttaaa tttattgccg gattaaattc ccagtttttt gcggatgtct
     7381 tttggcactg catccttgtg aacaaggatg ttgatagttt tgctcatcac gaaaggttta
     7441 ctcacatacc aggtgccttt gtacttgttg tcggttcccc aggaattttt gaccatataa
     7501 taaggtgtac cattctgatc tttggctatt ccgtaaatga gcatgccgtg gtcatcggtg
     7561 gtttgatagt tgtcaaaacc tttctgacga ctttcctgag taactttttc ttcaatcatc
     7621 ggtcctttga tgtcgtactt catatctttt tgcttaccaa cccattttgc ctgatctgtt
     7681 ccgttcaggt tttctgcttt tgtttcggga agtaatccga tgccgttacg ggtaaatcct
     7741 ttttcgctta catcggatcc ccaggcaacg gtgtaaccat tttcaacagc ataatcgaaa
     7801 atccggtcaa attcatcaag cggaatatta tatgattcag cccagcgcca gttgtcgggt
     7861 atttcgatgg caaattttgt gtaaaacgga tggtgagtaa atgaagtgat agaaacatag
     7921 tcatccatgt tcagacccaa cgaatttgca taacttaccg gagtgtattt ttttccgttt
     7981 actgtaaatt cagtcggcag ttttccaaga tatgcattca aaattccttc gaaagccggt
     8041 ttccaggcag aggaaatttt tttgttcgga ttttttacca ttgtagtcat aaaggcaccg
     8101 gtaagtgcgt ccagttcgcc gtgattatgt tttgtttccc cgtagttcag tccgttcatc
     8161 accgagtcgg gtatcattcc gtaagttttc cagacataca catcatcgta aaacgaaccg
     8221 ccgggagcaa aattgctggt gccgttcatg cgcaagtatt tatcggcttt gtcggagtag
     8281 gagtgatgaa ccacaaacat ttcggataaa tctacggctg gttttcccag gcgcagtaat
     8341 tcagattcga agaatgccaa tcccgaaaat gaccagcagg tgctggagtt tgcctgattt
     8401 tttacgggtg tgattttgtt ttcttttact actgtgaagt taaatgtggt atcgttgtag
     8461 ttttgggctg cgagtgatgc tgtcatacaa agcatcaggg aataaataag tagtcgtttc
     8521 atgttgtttg tgaatataga ttaaatgcaa tatcaggctt cagccacata tacttatgcg
     8581 gcagaaacct gatggattat tagtgttgat tattttttag gaatatttgc cagtgtgaga
     8641 agcaggaaat cccagaattt ttgaacggtt gggatgttga ctttttcggc cggagaatgc
     8701 gggaatcgga tggtaggacc aaatgaaatc atatccatgt aggggtaatt ccgccccaga
     8761 attccgcact caagtcctgc gtggataatc atgatccggg gagttttgcc gaaattcttt
     8821 tcgtagatat ttgtcatctc ttttagaata ggagagtcga ggttaggttt ccatccggga
     8881 tagccaccct tgaactccac ttttgcaccc gccagtgcga atacgctttc caccatcgat
     8941 gcaagttctt ccttgcggct ttctacagag cttcgtatca ggcagctcac cccgatcgtg
     9001 ttgccttcgg agcgaacgat tgccaggttg ttggaagttt ccactacatc gggaacttcg
     9061 ggtataaagc ggtaaacacc gtttggacag gctgtgatag catttatcag gtcatcctga
     9121 acttcttcgg gcattaatcc tttcaccttg tctgttcgtt cggctgtaaa actgatacca
     9181 tcttccacgc cttcgaattc tgtgcggaaa agagcttcat attcaccaac cagctccatc
     9241 agttcgtcaa caccttcttg tggaacggtt atggcagcaa aggcttcgcg cgggatggca
     9301 ttgcggagtg ttccgccttc gatgcaacac aggcgagcat cgtaatctgc cactgcttct
     9361 ttcagaaaac ggaacataag tttgttggca ttggcccgct gcaggtgaat atctacgccc
     9421 gaatgaccac ctttcaaccc tgtgagtgaa agtttgaaag caacatcgcc ttcgggtact
     9481 tccacttctt tgaatttgaa tgtaatatct gcatcaatac ctccggcgca gcctacgtag
     9541 agttcgcctt catcttcgga gtccatattg ataagaatgt cacctttcaa aaaatcgggt
     9601 tgcaggccga aagcgccaaa catgccggtt tcttcatcca cggtgataaa catttcaata
     9661 gccggatgag ccacacccgt tgattgcatg attgccattg cggcagctac cccgataccg
     9721 ttgtcggcgc cgagagttgt ctcgttggca gtcacccatt caccatcgat gtgtgtttgg
     9781 atgggatctt tttcaaaatc atggttaaca tggctgtttt tctgaggaac catgtccatg
     9841 tgagcctgaa gaataactcc tttgcggtct tccatacccg gtgtggccgg tttgcgtatc
     9901 agcacattgc caatttcgtc acgaagggtt tccagtccca gcgatttacc aaaatcttcg
     9961 agatatttac cgatttgttc ctttttgccg gagggacgag gaacctgtgt gattccgtgg
    10021 aaattttccc acaaggcttt gggttctaaa ttccggatgt catttgaatt catagtcgta
    10081 attttttatg ttgttatttt gtattactga gcattttttt tctatttaca aatatcatca
    10141 attattcttt caaatttata ttttatatat ttattttatg attattgaag gttctttcaa
    10201 cattggaaga tcacgcgtat atcttatgtt tgatatttac tgattgtttg attgttggaa
    10261 taattttggt aattttgcgt tttcaaaaaa gaattaattt tcaggtaaaa aatcatatta
    10321 tgcaaaacaa tttcagattt atgtccgctg atgaagcagc agctttcatc aacaatgggg
    10381 atattgtcgg tttcagcgga tttaccgcag caggttgccc gaaagtagta cctaccgcta
    10441 tcgctaaccg tgcaacagca tttcacaacg aaggaaaaga gtttaaaatc ggaatgtaca
    10501 ctggcgcttc aaccggcgat tccctcgacg gagcactcgc ccgtgcgaaa gctgtgaatt
    10561 tcagaactcc ttatcagtca aacaaagata tgcgaaacgc cctgaacagt ggtgaggtag
    10621 cttattacga ccttcatctg tccgcgctag cactgagttt acgctatgga tttattgctc
    10681 ccccaaaatt tgctatactt gaaatttgcg acctcacacc agaaggagaa gttgtactta
    10741 ccgccggtgt aggtatctca cctacgattg tcaatcttgc tgaacacata attctggagt
    10801 ggaatcatta tcatccgaag gaattgaagg gatttcacga tatttatgaa gcttccaatc
    10861 cgccgtcgcg ccgcgaaata ccgatctata caccctccga ccgcattggc accacaacgc
    10921 tgaagttgga tccgaaaaaa attctttgtg ttgttgatac caatcagatg gatgaagtgg
    10981 gcggatttac tcctgttgat gaagttacag cccgtattgg tgagaatgtg gcgactttcc
    11041 ttgcttccga attgaaacag aatagaattc cgaaagaatt tttacctctg cagtcgggtg
    11101 ttggaaatat tgcaaatgct gttttggctt cacttggcgc caataaggat attcctgctt
    11161 tcgaaatgta taccgaagtt attcaggact ctgttattga tctgatgaaa cagggcaggg
    11221 tgaaatttgc cagtggttgt tcgcttaccg tgtcgcccga tatgctgaaa gaagtatatg
    11281 ataatctcga ctttttccgt gataagatag tacttcgtcc tcaggaaata tccaatagtc
    11341 cagagattgc acgtcgtttg ggcctgatct ctatcaatac cgcactcgaa gccgatattt
    11401 tcggaaatgt aaacagtaca catgtgcttg gaagtaaaat gatgaacgga attggtggct
    11461 cgggagactt tacccgcaat gcctatttct ccattttcac aacaccatct acagccaaag
    11521 gcggaaaaat cagcgctatc gttccaatgg tttctcacaa cgaccactcc gaacattcgg
    11581 taaaagtgct gattaccgaa catggtattg ccgacctgag aggaaaatcg cccattcagc
    11641 gtgctcatct catcatcgag aactgcgttg atccggctta ccgtcagctc atgtacgatt
    11701 acctcaaact gggtgaaaag aatggtcata ctcctgtcaa tctggctgca ggatttggta
    11761 tgcacttaca gttcgccaaa agtggtaaca tgctcgatac taaatgggaa gattataccg
    11821 aataattttc tttgaaatat tttcatgcaa aagagacttt ttcgggtctc ttttgttgtt
    11881 tatggtaaat tattaacctg tcaaattcaa aaaatcatga aatcagacat agaaatagct
    11941 catgaaatag aactcgaaag aataaaaaat gtggctcaca agatgggcat tcatcgtgaa
    12001 tccatcatct cgttcggacc gtacattgca aaaattccaa ttagtgtgat tgacagcaaa
    12061 aagattaaaa aaagcaaact gattttggtc accgctatca ctcccacaaa agccgggatc
    12121 gggaaaacca ctgtgtcaat cgggctgagc cttggattaa ataagattgg aaaagttgct
    12181 gctgtggcac ttcgtgagcc ttcgctgggt ccctgtttcg ggatgaaagg aggcgctgcc
    12241 ggtggtggtt atgctcaggt attgcctatg gaaaatatca atctgcattt cacgggcgat
    12301 tttcatgcca ttacttcggc caataatatg attgctgcac tgctggacaa ctacatgtac
    12361 catcatcagc atcaggctga cggaataaaa caggttttat ggaagcgtgt attagatgta
    12421 aatgatcgca gtttgaggaa tgttgttacc ggtttgggtg gactcaccaa cggcattcct
    12481 gcagaaacag gttttgatat taccccggct tccgaaataa tggctatact ttgtctgtct
    12541 gaaaacgaag atgatttaaa gcgccgtctg gagaaaatac ttcttggata tacctattcc
    12601 gggaaaccat ttactgtgaa agatttaggt gttgcaggct ccattgctgt actgcttaaa
    12661 gatgccatgc atcccaatct ggttcagaca accgaacata ctccggcttt cgtgcatgga
    12721 ggaccctttg ctaatatcgc acatggatgc aactcggtgg ttgccaccaa aatggccatg
    12781 aatttttgtg agtatgtagt gaccgaagcc ggttttggcg cagacctcgg agccgaaaaa
    12841 tttatgaata tcaaatgccg gaaggcagga attcaaccag ttttatctgt gcttgtagta
    12901 accacccagg cacttaaaat gcatggcggc gtgcaggtga aagatattaa gctgccgaat
    12961 attgaaggtt tacagcgggg ttttgagaat atggataaac atattcagaa tttacattct
    13021 tttggtttgc aagtgcttgt ttcattcaat aaatatgact ttgacagcga agaggaaata
    13081 gagttgctta gcaatcattg taaagagatg aatgtcggat ttgctgtgaa tgatgcattt
    13141 actaaaggag gtgaaggagc tgttgagctg gcttctaagg tggtggaaat gacaagtgga
    13201 aagtgcgaaa agacaataca ttatacatac aaggacacgg attccattca gcgtaaagtt
    13261 gaaaaaattg ccatgaaact ttacggagca aggagagtga tattttccga aaaagccctg
    13321 aaaatgatac cgaaaatact ggaaatggga ggtgcggaat atccggtctg tattgccaaa
    13381 acacaatatt ccttttcgga cgacgaacgt ttatatgggg tagccaaaga ttttgacttc
    13441 catgtgaatg aactggtgtt gaataatgga gctgaattta ttgtagcgat tgccggaagc
    13501 ataatgcgta tgcccggtct gcccaaagaa ccacaggcag caaatatcga tttgatagat
    13561 ggtgaaattg tgggattaag ttaactttaa tagtcacaaa tatagaaatc cggtacagat
    13621 aatgtcccgg attttttcaa tccggataat aaatccccat agtatttaag gcgggattga
    13681 ttagctctcg ctgataagtg ccattattta ctctttattc ggtatatcaa cagggacggg
    13741 ggtaaattat ttattcatct ccaaaataat cattgtcaat acgttttatc tcaaatgtat
    13801 tgactgtcga aacggcaaga ttgtggtcaa tcattaattc ggctaattgt tcaccgtcaa
    13861 ttaatacgat ttttgtttca ttacgtggtt ggtagtcacg tgcatcattt gtatatcgtg
    13921 aagtcgtaat gaaaacacct ttttttgctc cttgtcctgc aagagcacct acaaactttt
    13981 gaatttctgg tctgccaaca acattttccc atctcttggc ttgtacataa attacgtcaa
    14041 gaccaagttt atcttcttta ataattccat caattccttc atcgccagaa cgtccgattg
    14101 aacgtccagc gtccttaatt gaaccaccat aacccatttt tacgagcaat tcaacaacta
    14161 atgtttcaaa gaatgaggga gtactacttt taattttgaa aagtaattct tgtgctagac
    14221 tctttcttat agtcaaataa ctattttcta ttatctcctc aggtgtcgca gaaatttgtg
    14281 aattttcagg atcagcagaa gagtcttcat catgtgttcg tcctgtcctt tcttggtaag
    14341 caggaatagt ttttaaaact cgcaaattaa tttctatagg attagtgttg agaagttgtt
    14401 tccccgcttc agtaattcga aatgaacttc tttttgtgtt ttcaagaagt ccagccattt
    14461 ttaaatgtga tttcgtccag gcaacacgat ttggaaatgt cttttgtgaa ccacttggaa
    14521 gatattcgtc taattccttg tctgtcaagt tgaaatgtgt cgccaacttt tggtttgtct
    14581 caatagttgt atgttcttct ccgtcagaaa taatctttaa gaacggaagc ataattgttt
    14641 gaaaatctgg tatcatagtt atgttgtgtt tgtttttgag ctttacgata actttatctt
    14701 tgcgctaact ttagtatata tctcgtgttt tcctttatgc tttttagtat ccaaaatagc
    14761 ttattcgctt aaaatcagat catgtctctt ctagtcattt tcaatactca cctccaaaga
    14821 taaaggattg ttttatttta ttatcaatat tctatattat ttttaattgt aaacagtagg
    14881 aagtatggtg ctaaggcatt gcttgctatc ctgcttccgc ctccaatagg ataaaaaaag
    14941 acaaatttaa ttcagcccaa tggtaaagcc ctgggttatg ttttttcggt ttgaatgatt
    15001 atatttgcaa tacataattt taaatcagac ataagatgaa aaacgctaaa ttattaattt
    15061 tattaagttt tgttctttgt ttttcgggca taagcgctca ggaatctcct tcaaaagagt
    15121 atcaggataa ttacaaaaaa gtaatggacc gaacagcctg gtggcgtaac gatcgttttg
    15181 gaatgtttat tcatttcgga gcatatgccg ttgctgcaag aggtgagtgg gtgaaatcta
    15241 atgaagaaat taccacggag agctatcaga aatatatcga tgagtttaat cctgtcgatt
    15301 ttgatgcccg caagtgggca aaaattgcca aagctgccgg aatgaaatac gccgttctca
    15361 cagccaaaca tcacgacgga tattgtatgt ttgatagtaa actgaccgac tataagttat
    15421 ccacccgttt cggcgggcgc gatctggttc gcgaatttct ggaagctttt cgcgctgagg
    15481 gaatcaaagt cggactgtat tattccatta tcgactggca tcatcccgat tatccgaatg
    15541 tcgggaatca tcctcaacgc aatgacaaag aatacggaaa acgtaaattt aactgggaca
    15601 actacctgaa atatatgcac gggcaggtgg aagaactggt tcgggattac gggaaaattg
    15661 atatcatgtg gttcgattat tcatttgatg agtatagcgg tgaaaaatgg aaagccaaag
    15721 aactggttca aatggtgcgc aaatatcaac ctgacattat tttagataat cggctgaaac
    15781 ataacgatgg aatctcttcg aagcaacgga cgatttcgac acttggtgat tttgaaaccc
    15841 cggaacaggg aatacccgat gtgccgctta ctgacaagtt cgggaatgca attccctggg
    15901 aaacctgtct gacgttgaac ggagtatggg gttacaatga gtctgataaa aactggaaat
    15961 cgccggagtt aatcgttcat acactagtca actgcgtgag taaaaacggc aatctcctgc
    16021 taaatgttgg gcctgatgcg cgcggaaata ttccacagga atccgtaagt atccttaaag
    16081 aagttggaaa ctggatggct aaaaatagtg aaagtattta tggttgcggt tcatccgcat
    16141 tagccaaacc cgactggggc cgttatacat caaaagggaa tacattgtac gctcactggt
    16201 tatatcctaa cattggtgat atcaataccc gcggagtaga cggcgataaa gtgaaagcgg
    16261 tatatatgct ctgcacgggt gcagaactgc cttatcaaaa atcgtggtgg ggtaacaccg
    16321 aacccggaaa tctttttatt tctgttggca gaaatcccca gaagccatat aaatatgata
    16381 cggtactgaa aattcagttt aaataaccag agctattctg atgtcgcacc tacagagttg
    16441 taaaattcaa ttgcttttat aattcttaat tggtaactaa caaagagaaa tcccataagg
    16501 cttaactgga cacaaccccc ggtttcaacc gggggttgac tataaaaaac gcaacatgaa
    16561 cttcggagtg gttttgccaa tacctgatta gcaaagacaa ccccaatatg aggttgataa
    16621 tcagaggaat ttgtaagttg ttttgattac agaataatgt tgaccggatt tatttttgaa
    16681 aaataatacc gtatattaaa tgatcgtgtg ctattctctg attaaaccat tatcctgagt
    16741 ggacgatatc tttttgtgaa ttctccattc ttcagttttc ctgatgattt ttattcaaaa
    16801 gttaagataa caaaagatta tttgtttaac gattgagaag aaaatgatcc gcactaataa
    16861 tcaggctttc tgcggtcgaa aagaaccggc ctggaatttc tggctgaacc gaaccgccct
    16921 cctgaaaata taggaaacaa aaaaaggaaa tgtttttcaa cacttccttt ctctttgttg
    16981 cggaggctcg actcgaacga aacgtccgtc gggtgacgga tatgagccca acaagcttaa
    17041 agtattttgt tatgaataaa ttctctgcct t
//
LOCUS       DAOPYB010000051        16942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3583_length_16942_cov_9.663291, whole genome shotgun sequence.
ACCESSION   DAOPYB010000051 DAOPYB010000000
VERSION     DAOPYB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16942
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3583_length_16942_cov_9.663291"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1866
                     /locus_tag="PKH58_05700"
     CDS             217..1866
                     /locus_tag="PKH58_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX88554.1"
                     /translation="MQLSDRTLSDWLEYWAAETPNKEYIVYSDRNLRFTWQSFNERVN
                     NTAKGLISIGVTRGTNVGIWAQNVPDWLTFLYATAKIGAVAVTVNTNYKTEELAYILK
                     NSDMHTLCLTEGIPGSDYIEMVYELLPELKTTQRGRLNSEQFPEMKNVVYIGQEKYRG
                     MYNTQEILLLGANQPEEEFLRLRNQTACHDVVNMQYTSGTTGFPKGVMLTHHNIANNG
                     YLTGVHMKFTPEDKCCICVPLFHCFGVVLATMCCLTHGSTQVMVERFDPLLTLASIHK
                     ERCTVLHGVPTMFIAELNHPMFSLFDVSSLRTGIMAGALCPMELMKQVNEKMFMDVTS
                     VYGMTETSPGMTQTRVDDTFEVRCTTVGRDYEFTDVKVFDPETGMECSTGVQGEICCR
                     GYNVMKGYYKNPEATAQVIDADGYMHSGDLGVKDENGNYRITGRIKDMIIRGGENISP
                     KEVEDYLYKMPEIQDVQIVAVTSPKYGEDVGAFIILKEGCELTREDVRDFCKDHIAKY
                     KTPRYVFFIKQFPMTGSGKIQKFRLREMALEMCEKEGIVIV"
     gene            2051..2755
                     /locus_tag="PKH58_05705"
     CDS             2051..2755
                     /locus_tag="PKH58_05705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX88555.1"
                     /translation="MHSPYLFRFTQNVIYDKNPFYIFPAIEKLRAQLRRDERFLHLTD
                     FGTGNNSVLKVRDIAKKSLKKPNRARLLFRTVHFLQAKIVVELGTSLGITTAYLASPN
                     SDLECVTFEGSEEVAAVARENFEKLGLKNIRQITGNIDNTLEPFLQENKTLDVVFFDA
                     NHRQEAVLRYFDLCVKHITRNSVFIVDDIHWSTDMEDAWFSILNHPEVQSSIDLFDCG
                     LVFFDKYLPKKHFKMR"
     gene            2883..3434
                     /locus_tag="PKH58_05710"
     CDS             2883..3434
                     /locus_tag="PKH58_05710"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNX88556.1"
                     /translation="MKIYTKTGDAGQTGLIGGTRVSKADIRLEAYGTVDELNAQIGLL
                     AAEELKPEHLMFLQNIQNLLFTVGSNLATDTSKTEYRVASVMKAEYIEMIENEIDKID
                     LLLPPLTNFVLPGGNRKSALCHVCRTVSRRAERRIIELNSMYEIENKIIVFVNRLSDY
                     FFVLSRLMLMEEQKQEISWNQPK"
     gene            3510..3731
                     /locus_tag="PKH58_05715"
     CDS             3510..3731
                     /locus_tag="PKH58_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNX88557.1"
                     /translation="MYWTLELASKIEDAPWPATKDELIDYAIRSGAPLEVIENLQEIE
                     DEGEIYETIEDIWPDYPSKEDFFFNEEEY"
     gene            3873..4853
                     /locus_tag="PKH58_05720"
     CDS             3873..4853
                     /locus_tag="PKH58_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX88558.1"
                     /translation="MNITFENKIIKRILLLIFSIVCNVSAFSQFEAQISQYMLHPATY
                     NPAAVGEGGMINVTGQHKIQWLGMPGAPQTTFFTINAPFKLSKSVTHGLGIKFLNDKI
                     GAYTNQSAGLQYAFKKNLGEGVLSLGVDLGFVNVGFVADSVRKNDINSEYHDFSGDMT
                     IPTADENGMSLDMSLGAFYSAPKYYAGLSYVHLNNPVVKLGEKSNFQVRGVTYLTGGY
                     NASLANPNLILRSSALLKTDFTTWQAELSSRLEYDKKFWGGLSYRYQDAVIVLAGLNL
                     MSGFTLGYAYELPTGKMLTVSSGSHELLLSYSFAFDMSKNKNKYKSIRIL"
     gene            4900..6267
                     /locus_tag="PKH58_05725"
     CDS             4900..6267
                     /locus_tag="PKH58_05725"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX88559.1"
                     /translation="MKRFLPILLVASVFVSCNKSSGELVGIGNTGTFHEANPYGMVFV
                     KRGSFMMGANDQSAFGGGNDRSVNVTVDAFWMDETEITNDEYKQFVTWVKDSIALRKL
                     VIAGKDEFRKQFKNQSDDAAPEEARLNWKAKIPWNSKDEDVQEILSSMYYEGNNLLGS
                     RKQIDPTKLQFKYEWLNTDQAALPGNKFNVNTGAYYEGAKARVDSSYVDENGVIHNVT
                     ITKKLRSRRDLISNKIVNVYPDTISWLRDFQYAYNDPKMKMYFSHKGFSQYPVVGVSW
                     EQAQAFCQWRTNYFNNANVVGGQDYRLPTEAEWEYAARGGRDLSLYPWGGNYVRDAKG
                     CFLANFKPMRGSYTDDTGGVTMKVGSFAPNNFGLYDMAGNVSEWTSTAWGGSSNILVS
                     DLNPNYQYNAKNDDSDVLKRKVIKGGSWKDIAYYLQCGVKSYEYQYESRPYIGFRCIR
                     SYNGE"
     gene            6312..7205
                     /gene="gldL"
                     /locus_tag="PKH58_05730"
     CDS             6312..7205
                     /gene="gldL"
                     /locus_tag="PKH58_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNX88560.1"
                     /translation="MGSEHSTFYRMWNSNRGKKIISAAYSLGASVVILGALFKILHLP
                     GANYMLMAGMITESVIFALGIFDTPHKEYDWGQIFDFKNPEKINIGAAGGLAPAATQA
                     VAPTRQVGLSYTETISDDEVKKLSEGIKNLSSTADNLQVLANVAVAASGLTRQMETAS
                     EVTGSYANTQQKLNSTADKLSASYQGISSEMDAITSSTKNYAEKVADVNKSLASINSI
                     YEIQLRQIQSQSEGLNLQSENIRAITGHMDEMTSDISKMKEATSLALNDSQKYQAATR
                     KLSKQVEDLNAVYGNMLNALN"
     gene            7238..8761
                     /gene="gldM"
                     /locus_tag="PKH58_05735"
     CDS             7238..8761
                     /gene="gldM"
                     /locus_tag="PKH58_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HNX88561.1"
                     /translation="MSGAKNCPETPRQRMIGMMYLVLTAMLALNVSSEILEGFGLVDR
                     GLKNTIESAEIRNKGMYADFQEEAKKNPQKIGEWLKLAREVEKKSNDLYNFIGDYKYQ
                     ILKISDKEKADKKAENIIARDNLDAAGQYSLVGGHYKELKQKIEAYRELLIQMSEGDR
                     AKQNMYKKIFDTSNRFGKTWQSSMFESMPVAAVITMLTKYQSDVRSAEAETVQYLRTR
                     ADLKDFRVNKLEAFVIPESSYILQGGTYTAKIVLAAIDTTSSYAYSVNGGRLSGNVYR
                     AGGSTPGTFKYTGSISVPNNEGDIKSYPFSSEYVVGKPSATLSNIDLNVVYKGIDNKF
                     SISAPGIPDENLRVSAEGASVGKAGNYFVIKPTRDGEIRINVMAVMQGKTVSMGSSVY
                     RVKYLPDPTAFFSYTDAGGVPRTTQDAQLSKKAIRSGRLIASYGPDELVKANFTITSF
                     TVHTIFGTATSSSSEFSPRQLSLMTELEGGDVVTLKNIKAVGPDGKVRSLGLIQIEI"
     gene            8793..9692
                     /gene="gldN"
                     /locus_tag="PKH58_05740"
     CDS             8793..9692
                     /gene="gldN"
                     /locus_tag="PKH58_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNX88562.1"
                     /translation="MKKYSVFLVIISLLLIPCKSSAQKTLQFFDEIGVEKLQTAEMDA
                     MADTIAVVNHRWDDIVWSRTVYRVIDMRDKQNFQLYFPIRPNEQYRSLFRVMLDAVAN
                     GVNVYKRDPRDIRPSWKDVLTGYELSRVFAYDESTENNLIQVDTLTEQRSVGIEQYSR
                     YVRNQLKFLIQEKIFFDKHTSRMYTKIMGIAPMYALHPDNMDSKESMTYFRNSVICWF
                     AYDELRPYLIKQFVVPKGNDTERMTFDDFFSQKMYASYLLGESDTYGRMLLEYAVDED
                     ALKAEQKRIETELVNFEEDLWEY"
     gene            9796..10446
                     /locus_tag="PKH58_05745"
     CDS             9796..10446
                     /locus_tag="PKH58_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX88563.1"
                     /translation="MQEIILSDNQDITRAGLMQIFSSEFTCHFVEAKNKKELINCLEN
                     CTEAIVVLDYTLSDFQRVEDLLMVSARFQDAGWLLISNELRDEFVRNIYLNAENFSIV
                     YKDCSTEELLMAMRGLQKGQRYISNTVSKNLLESAFRQQTGDSNTKLTATEKEILKDI
                     ASGKTTKEISDQRHVSTHTIVSHRKNIFRKIGVNNVHEATKYAVKAGLIDLADYFI"
     gene            10460..11521
                     /gene="mnmA"
                     /locus_tag="PKH58_05750"
     CDS             10460..11521
                     /gene="mnmA"
                     /locus_tag="PKH58_05750"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNX88564.1"
                     /translation="MNIATLISGGVDSSVVVHLLKESGITPSLFYIKIGMDDDELLHC
                     SSEEDIEMASLVARKYGCKLEVVDLHKEYWDNVVAYTIDKAKKGLTPNPDVMCNKLIK
                     FGVFEQRVGISFDKTATGHYATTIEKDGKVFLGTAKDPVKDQTDFLAQINYLQVSKLM
                     FPIGHLMKSEVRKIAENVQLPSAKRHDSQGICFLGKVNYNDFIRRYLGVKQGPIVELE
                     TGRILGHHNGYWFHTVGQRKGLGLSGGPWYVIRKDVESNIVWASKGFEVESQYGYEFA
                     MNDFHFITENPWKDTEESVDITFKIRHTPEFTKGKIIKTATGYRIVSEDKLQGIAAGQ
                     FGVVYDEASGICIGSGEIV"
     gene            complement(11531..11914)
                     /locus_tag="PKH58_05755"
     CDS             complement(11531..11914)
                     /locus_tag="PKH58_05755"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNX88565.1"
                     /translation="MKKEKLTIEYPLNNASEAILWKMIGTPLGLAEWFSDAVIVEGNE
                     YTFSWEGHEQTACLKQVKEGKLICFQWEDDKDTEAYFQLEIVSLDLSGHVGLIVTDYA
                     TPGEREDAILLWNKQIENLCRIGGM"
     gene            complement(12015..12497)
                     /gene="cdd"
                     /locus_tag="PKH58_05760"
     CDS             complement(12015..12497)
                     /gene="cdd"
                     /locus_tag="PKH58_05760"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNX88566.1"
                     /translation="MQYISHEIKISVFQPDEIPSEYISLIEEAKKQTQKSYAPYSEFH
                     VGAAVLLENGIVVGGNNQENSAYPSGTCAERTAIFYANSQHPDIPVKLLAIAAFTNGK
                     FTSEPVTPCGACRQVLLETENRYGQAIKVILYGTDKIYVFDSIKQLLPLSFGKDSLTL
                     "
     gene            12629..13567
                     /locus_tag="PKH58_05765"
     CDS             12629..13567
                     /locus_tag="PKH58_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNX88567.1"
                     /translation="MNRSLIFIISLISFSQTFLAQNRSESYLNYIEKYSKLAVEQQKR
                     YRIPASITLAQGLLESAAGMSELSRTSNNHFGIKCHNGWEGEKVFHKDDHAEPDCFRK
                     YKEVSESYEDHSLFLSNRQHYAFLFDLNPTDYKEWAYGLKKAGYASDPAYPVKLIKII
                     EDYELHQFDLGGKVDYLHSRTEKAVRTHESNRLKTSAGIGDHDLFRNNKVICIFAHTS
                     DSFESLASEYNIPEKKLLQYNDLSESTDIVPGTIIYLKTKKKKAAAGFLQHVVKEGEN
                     MYRISQKYGIRLEMLYKLNGMPYSEGAETGMILKLR"
     gene            13572..13862
                     /locus_tag="PKH58_05770"
     CDS             13572..13862
                     /locus_tag="PKH58_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNX88568.1"
                     /translation="MRRKNTEKLSDILGVVLKQNHLDEKLYETRVLKSWPGVLGDNVM
                     KYTSDIYFNRKKLYVKLTSSVLRQELFITREEIRNSLNNFVGFPVVKEIVFL"
     gene            13894..14466
                     /locus_tag="PKH58_05775"
     CDS             13894..14466
                     /locus_tag="PKH58_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88569.1"
                     /translation="MDYEAVISHRLTKEEVLIVRDELISEKESFSDFFQLIFQKPVKT
                     AWQVAWICEKVSEADVTMFTDEDYHQLMDFTLTNNEDGLQRLCLSIILNLPLRKPVSV
                     EFINLCFDQMISPRKPVGVQVLSMKILRKICIAVPDLGDELLVILENTDDLTYSPGFI
                     SARKHVIKSLKTNSKGGFGVNGRLKDYAEW"
     gene            14521..16065
                     /locus_tag="PKH58_05780"
     CDS             14521..16065
                     /locus_tag="PKH58_05780"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNX88570.1"
                     /translation="MSLKKNIIALRQKKAIVEKGGGDKALEKQMAMGKMPARVRILAL
                     LDEGSFHEYDMFVEHEEQNFGMANKELPAEGVITGTGTINGAPVCIYAQDFTVMGGSL
                     GLKHARKITKIMDHALKMKIPCIGINDSGGARIQEGIGALAGYGEIFFRNTMASGVIP
                     QLSVILGPCAGGAVYSPALTDFVFVVENISKMFITGPNVIETVLGEKISQEDLGGARV
                     HAEITGNAHFYATSEMECFKQIKTLISLIPHNNEVKAKSVAPKDPKYRKKIENIIPSD
                     PKQPYDVREVIFALTDESKFFEVQELWAANIVIGFGRMNGETVGFVANQPLYLAGVLD
                     CDASDKAARFIRFCDAFNIPIITLEDMPGYLPGVDQEHAGVIRHGAKMLYAYSEATVP
                     TMTIILRKAYGGGYIAMNSRHLGADFVYAWPSAEIAVMGPEGAANIIFRKEIMEAEDE
                     DAMRQQKVKEYVEKFANPFVAASKGYIDAVIEPSETRDLLLHTLEVSRLKEEHRPMKK
                     HGIPPF"
     gene            16132..16746
                     /locus_tag="PKH58_05785"
     CDS             16132..16746
                     /locus_tag="PKH58_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNX88571.1"
                     /translation="MAKKENNTDSTGKAQLNEQKANRKQTIERAKKIQAARKAEEAKK
                     KAAEKQIAQKQKKEEVKPTVAAPSVNVTDADVRPMPENEKKTINTQVKSMSAEEKKEP
                     EYVDFAITARKYKTLLTNKYKNRKFWINPSPYEIQSYIPGTIIEVCVKEGKTVKEGDP
                     LLVLESMKMQNRIDMPFTAKIKKINVKAGERIPKDFLMVELEQA"
BASE COUNT         5334 a         3310 c         3795 g         4503 t
ORIGIN      
        1 gaaggtgaca gcatttacta cgattcgatt gtggatcatc tggttaccac ttccggtgat
       61 caaccggcgc aaatcctcgg aatcgtgtat tcccctttct agaatacacg aataacacga
      121 gatcttcacg aatttcacga attaaaaacc tgatcgtgtt gacttcagat ttcaaataaa
      181 cctctgcatc agggttaaac ttcaaacttc taaaaaatgc aactttctga cagaacatta
      241 agcgactggc tcgaatactg ggcagccgaa actccaaata aagaatatat cgtttattcc
      301 gaccgcaatc tgcgttttac ctggcaatcg ttcaacgagc gggtgaacaa taccgcgaaa
      361 ggactgatat cgataggggt gacacgggga acaaacgtgg gtatatgggc gcagaatgtg
      421 cccgactggc tgacatttct ttatgccaca gctaaaatcg gagcagttgc cgttacagta
      481 aataccaact ataaaacgga ggaactggct tatatcctga agaattcaga tatgcatacc
      541 ctctgtctta ccgaaggaat tcccggaagc gattacatcg aaatggttta cgagctgctt
      601 cccgaactga aaacaaccca gcggggaaga ctcaatagtg agcagtttcc cgaaatgaaa
      661 aatgtggtgt atatcggtca ggaaaaatac cggggcatgt acaacacgca ggagatactg
      721 ctgctgggag caaaccagcc ggaagaggaa ttcctgcggc tgcggaatca gaccgcttgc
      781 cacgatgtgg tcaacatgca atacacctcc ggcaccacgg gatttccgaa aggtgtgatg
      841 ctgactcatc ataatattgc caacaatggt tatttgaccg gtgtgcacat gaaattcacg
      901 ccggaagata aatgctgtat ttgtgtgccg ctttttcatt gtttcggtgt ggttctagcc
      961 actatgtgct gcctcactca cggtagcaca caggtaatgg tcgaaagatt tgatccgctg
     1021 cttaccctgg cctccatcca caaggaacgc tgcacggtac ttcacggtgt tcccaccatg
     1081 ttcattgccg aactcaatca tcccatgttc tcactctttg atgtaagctc actgcgaacc
     1141 ggaataatgg ccggagcgct ctgtcccatg gaactgatga aacaggtgaa cgaaaaaatg
     1201 ttcatggacg taacgagcgt gtacggaatg accgagacct cgcccggaat gacgcaaacc
     1261 cgcgtggatg acacttttga ggtgcgctgt accaccgtag gacgcgatta cgaatttaca
     1321 gatgtgaaag tattcgatcc cgaaacgggc atggaatgtt ccacgggcgt tcagggcgaa
     1381 atatgctgcc gcggatacaa tgtgatgaaa ggatattaca aaaatccgga agcgacagct
     1441 caggtgattg atgccgacgg atatatgcat tctggcgacc tcggtgtaaa ggacgagaac
     1501 ggcaactacc gcatcacggg gcgtataaaa gatatgatca tacgtggcgg tgagaatata
     1561 tcgcccaagg aagtggagga ttacctgtac aaaatgcccg aaatacagga tgtacagatt
     1621 gtggcagtca cttcacccaa atatggtgag gatgtgggag cattcattat tctgaaagaa
     1681 ggatgtgaac tcacacggga agatgttcgc gatttctgta aagaccatat cgcaaaatat
     1741 aaaactcccc ggtatgtttt ctttatcaaa cagttcccca tgaccggcag cggtaagatt
     1801 caaaagttcc gtctgcgcga aatggccttg gaaatgtgcg aaaaagaggg aatcgttatt
     1861 gtgtaaaaaa gaaaacttgc agataatatt gcgttattcc tgttttttat ttcatttttt
     1921 tattttcttt ctgtattttt gttcttaatt ttgaacttac acaagctaaa ccggtaaatt
     1981 gaaagaaatt tcgtttataa tcaggtattt gattcatttt tttgaagcaa aaaatacaaa
     2041 aggacatgga gtgcattctc cgtatctgtt caggttcact cagaatgtga tctatgataa
     2101 aaaccccttt tatatatttc ctgcaattga gaagttgagg gcgcaactcc gtcgcgacga
     2161 acgttttctc cacctgaccg acttcgggac cggcaataat tcggtattga aagtgcgcga
     2221 tatagcgaag aaatcgctga aaaagccgaa ccgtgcccgg ttattattca gaacagttca
     2281 tttccttcag gcaaaaatag tggttgaact cggtacttcg ctgggtatca ccactgctta
     2341 cctcgcttca ccgaattcag acctggagtg tgtcaccttc gaaggaagtg aagaggttgc
     2401 ggctgttgcc agagagaatt ttgaaaaact cgggcttaaa aatatccgcc agattaccgg
     2461 gaacatcgat aatacactgg aaccctttct gcaggagaat aaaacactcg acgtggtgtt
     2521 ctttgatgcc aaccaccggc aggaagcggt attgaggtat tttgatctgt gtgtaaaaca
     2581 tattacccgt aattcggttt ttattgtgga tgatattcac tggtcaaccg acatggaaga
     2641 tgcctggttt agcatactga atcatcccga agtgcagtcg agtattgatc tttttgattg
     2701 cggactcgtg tttttcgaca aatatctgcc gaagaaacat tttaaaatga gataatagcg
     2761 atgaaacaac catgctgata atttactgca gattgtgaaa aaaaggtatt ggtgattggt
     2821 gattggtggc tggttattaa gttattgatt ggtagtcttg gttaaaaata aaaaatacac
     2881 ggatgaaaat atatacaaaa acaggagatg cgggtcaaac cggtctgata gggggaacgc
     2941 gagtttcgaa agccgatata cggctggaag cttatggtac agtggatgag ctgaatgctc
     3001 agatcggact gctggctgcc gaagagctca agcctgaaca cctgatgttc ctgcaaaata
     3061 ttcaaaacct cctgtttact gtaggctcaa acctggccac agatacatcc aaaacagagt
     3121 accgggtagc atcggtcatg aaggctgagt atatcgaaat gattgaaaac gaaattgaca
     3181 agattgacct gctactcccg ccacttacta attttgtgct tccgggagga aaccggaaaa
     3241 gtgcattgtg tcatgtttgc cgcactgtat cgcggcgtgc tgaacgcaga attattgaat
     3301 tgaacagtat gtatgagata gaaaataaaa taattgtatt tgttaaccgt ttatcagatt
     3361 atttctttgt actttcacgt ttaatgttaa tggaggaaca aaagcaagaa atatcctgga
     3421 atcaaccaaa ataacaaatt tattttctac ttttgcactc caatttatag attttaacga
     3481 tattaaccta attagatttt atttagatta tgtattggac actagaactc gcatccaaaa
     3541 ttgaagatgc accatggcct gcaaccaaag atgagttaat tgattacgcc ataagatcag
     3601 gtgcacccct cgaagtaata gaaaatcttc aggaaataga agatgaagga gaaatttacg
     3661 aaacaattga agacatctgg ccagattatc caagtaaaga agacttcttt ttcaacgaag
     3721 aagagtatta atgcgcctga cttaactctg attagtgcac tgtgatacgt atgttctatt
     3781 cagaaagaaa aaacaatttg gcagataata caattttttg agatctttgg agttttattt
     3841 ttcaggcaaa agatttttga aaaaagcagt tcatgaatat tacatttgaa aataaaataa
     3901 taaaacgcat actattgctg atattttcaa ttgtctgtaa tgtatctgct ttttcacaat
     3961 ttgaagccca gataagtcag tatatgttac atcctgcaac ctataatcct gccgcagtag
     4021 gagagggtgg tatgattaac gtgaccggac aacataaaat tcagtggttg ggaatgcccg
     4081 gagcacctca aacaacattt tttacgataa atgctccttt caaactgagc aaatcggtta
     4141 ctcacggatt gggtatcaaa tttctgaatg ataaaatcgg agcttatacc aatcaatcgg
     4201 ctggtttgca gtacgcgttc aaaaaaaacc ttggagaagg tgttttgagc ttgggcgtcg
     4261 atctgggatt tgttaacgtt ggattcgttg ccgacagtgt acgcaaaaat gacataaact
     4321 ccgaatatca tgatttttcg ggggatatga cgataccaac ggccgatgaa aacggaatga
     4381 gcctggatat gtccctgggt gctttttatt cagcaccaaa atactatgca ggcctttctt
     4441 acgttcattt aaataatccg gtggtaaaac tgggcgaaaa gtcgaacttt caggtgcgcg
     4501 gtgtaacgta tcttacagga ggatataatg cctcgttggc caatccgaat ttaatactgc
     4561 gttcatccgc tttacttaaa actgatttta ccacctggca ggctgagtta tcatcccgcc
     4621 tcgaatacga taagaaattt tggggaggtt tatcataccg gtatcaggat gcagtgattg
     4681 tgcttgcagg attgaacctg atgagtggat ttacactggg atatgcctac gaactgccta
     4741 ctggaaaaat gcttacggta agttcaggct cacacgagtt actactgtca tacagttttg
     4801 ctttcgatat gagcaagaat aaaaataaat ataagagtat cagaatatta taaaaacaat
     4861 caatagaatc aattctttat tattatgtac aaaataagaa tgaaaagatt tctgccaatt
     4921 ttattagtgg cttccgtatt tgtatcgtgc aacaaatcat ccggagagtt ggtcggaatt
     4981 ggaaatacgg gaacttttca cgaagcaaat ccttatggaa tggttttcgt gaaacgaggt
     5041 tcatttatga tgggcgctaa tgatcagtct gctttcggtg gtggcaacga tcgttcggta
     5101 aatgttacgg tggatgcatt ctggatggat gaaactgaaa ttacaaatga tgaatacaaa
     5161 cagtttgtaa cctgggtaaa agattctatt gctttacgaa aactggtgat agccggaaaa
     5221 gatgaattcc gcaaacaatt caaaaatcag agcgatgatg ctgcacccga agaagcgcgc
     5281 ctgaactgga aagccaaaat tccatggaat tcgaaagatg aagatgttca ggaaattctt
     5341 agctccatgt actacgaagg caataatctg ctgggctcac gcaaacagat tgaccccacc
     5401 aaactgcaat ttaagtacga atggctcaat accgatcagg ccgcattgcc gggaaacaag
     5461 tttaatgtca atacaggagc ctactacgaa ggtgccaaag cacgcgtgga tagttcgtat
     5521 gttgacgaaa acggtgtaat tcacaacgta accattacca aaaagctccg ttcacgcagg
     5581 gatttaatct cgaacaagat tgtaaatgta tatcccgata ccattagctg gctgcgcgat
     5641 tttcagtatg cttacaacga tccgaaaatg aaaatgtatt tctcgcacaa gggattttcg
     5701 caatacccgg tggtaggtgt atcgtgggaa caggcacagg ctttctgcca gtggcgtacc
     5761 aattatttca acaacgcaaa cgttgtaggt ggtcaggatt accgcttacc caccgaagcc
     5821 gaatgggaat atgcagcccg cggcggacgt gatctttctc tgtatccctg gggaggaaat
     5881 tatgtacgcg atgcgaaagg atgcttcctg gctaacttca aacccatgcg gggtagttat
     5941 actgacgata caggtggtgt gaccatgaaa gtgggatctt ttgcacccaa caattttggt
     6001 ttgtatgaca tggccggtaa tgtgtcggaa tggacttcga ctgcctgggg cggatcaagc
     6061 aatatactcg tttctgattt gaaccccaac taccagtaca atgctaaaaa tgatgattcc
     6121 gacgtactta aacggaaagt tattaaaggc ggtagctgga aagatattgc ctattacctg
     6181 caatgtggtg tcaaatcgta tgaatatcag tacgaaagcc gtccatacat cggtttccgc
     6241 tgcattcgtt catacaatgg cgaataaaac cagatacttg ttaaaaacaa tcacaataaa
     6301 aaaatacgaa gatgggaagt gaacacagca cgttctacag aatgtggaat tcaaaccggg
     6361 gaaagaaaat aatttcagca gcatatagtt taggggcatc ggtagtaatt ttaggcgctc
     6421 tttttaagat cttacacttg cccggagcaa attacatgct gatggccggt atgatcaccg
     6481 aatcagttat ttttgcttta ggtatattcg atacaccaca taaggaatat gattggggtc
     6541 agatttttga ctttaaaaat cccgaaaaaa taaatattgg tgccgcagga ggattggcac
     6601 ctgcagcgac acaggcagta gcacctacac gtcaggtagg cttgagttat acagaaacaa
     6661 tatcggacga tgaagtgaaa aaactgagcg aaggcattaa aaacctttcc agtacagccg
     6721 ataatcttca ggtacttgcc aatgtggcag tagcggcatc gggactcacc cgtcagatgg
     6781 aaacagcatc agaagtcacc ggttcatacg ccaatacaca gcaaaaactg aattccacag
     6841 ctgataaact gtcagcttcc tatcagggaa tttcatcgga aatggatgct atcacttcca
     6901 gtaccaaaaa ctatgctgaa aaagtggctg atgtcaacaa aagccttgct tctatcaact
     6961 caatctacga aatacagttg cgtcagatac aatcacaatc ggaaggactt aatctccagt
     7021 ccgaaaatat acgggctatt accggacata tggatgaaat gacatcggat atcagtaaga
     7081 tgaaagaggc aacttcactt gccctgaatg atagccagaa atatcaggct gccacccgca
     7141 aattgtccaa acaggtagaa gacctgaatg cagtatatgg taacatgttg aatgctctta
     7201 attaatcaga ccacaaaatt tttaaaagag agataaaatg agtggagcaa aaaattgtcc
     7261 agaaaccccg cgtcagagaa tgattgggat gatgtatctg gtgcttaccg cgatgcttgc
     7321 actgaatgtg tcaagcgaaa tcctggaagg cttcggactg gtggacagag ggttgaaaaa
     7381 tacaatcgaa tcggctgaga tccgtaacaa agggatgtat gccgactttc aggaagaagc
     7441 taaaaagaat ccacagaaaa taggtgaatg gctaaaactt gcccgggaag ttgaaaaaaa
     7501 atcaaacgat ctttacaact ttatcggtga ttacaaatac cagattctga agatctctga
     7561 taaagaaaaa gcggataaaa aagcagaaaa tattattgcc cgcgataatt tggacgcagc
     7621 aggacagtat tctctcgtag gcggtcacta taaggaactg aagcagaaaa tagaagctta
     7681 tcgtgaactt ctgatacaga tgtcggaagg tgacagggct aagcagaata tgtacaaaaa
     7741 gatatttgac acttcgaacc ggttcggtaa aacctggcag tcaagtatgt ttgaaagtat
     7801 gcctgtagct gcggttatta ccatgcttac caagtatcag tccgatgtcc gctcagcaga
     7861 agctgaaaca gttcagtatc tcagaacgcg agctgatctc aaagacttcc gtgtgaataa
     7921 actggaagct ttcgttattc ccgagtcaag ctatatcttg cagggaggaa cttataccgc
     7981 taaaattgta ctggctgcta ttgatacaac atcaagttac gcctattcgg tgaatggcgg
     8041 acggttgtcg ggcaatgttt accgggcagg aggctctaca ccaggtacct tcaagtacac
     8101 tggaagtatc tctgtaccca acaacgaagg cgatattaaa tcatatcctt tcagcagcga
     8161 gtacgtagtg ggaaaaccat cagccacttt gtcgaatata gatttaaatg tcgtatacaa
     8221 ggggattgac aataaattca gtatttcagc tcccggtatt cctgatgaaa accttcgtgt
     8281 gagtgccgaa ggtgcgtcag taggcaaagc gggtaattat tttgtcataa aaccaactcg
     8341 cgacggggaa atcagaatta atgtaatggc tgtgatgcag ggtaaaacag tttcgatggg
     8401 tagcagtgtg tatcgggtga aatacctgcc cgacccaact gcattcttct cctataccga
     8461 tgccggaggc gtgccaagaa ctactcagga tgctcagttg tccaaaaaag caattcgcag
     8521 cggacgcctg attgccagtt acggaccgga tgaactcgta aaggctaatt ttacaataac
     8581 atcctttacc gtacacacga tatttggtac agctacttcg tcaagttctg aattcagccc
     8641 gcgtcagcta tcactgatga ccgagctgga aggtggtgat gtggtaacat taaaaaatat
     8701 caaggcagtg ggacccgatg gaaaagtccg cagtctggga ttaattcaaa tagaaatata
     8761 aatcatacga aacgaattta caattcatca ttatgaaaaa atatagtgtt tttttggtga
     8821 ttatttcact gttgcttatt ccttgcaaat caagcgctca gaaaacgctc cagttttttg
     8881 acgaaatcgg cgtagaaaaa ctccagactg cagaaatgga tgcaatggct gatacgattg
     8941 ccgttgtcaa tcatcgctgg gatgatatag tttggtcacg cacagtttat cgggtgattg
     9001 atatgcgcga taaacaaaat tttcagctat attttccaat tcgtccaaac gaacagtatc
     9061 gtagcctgtt tcgggtaatg ctggatgcag tggcaaatgg tgtgaatgta tataaacgcg
     9121 acccaaggga tatcagacca agctggaagg atgtgctgac cggttatgaa ctttcccgcg
     9181 tattcgctta tgacgagtca acggagaata acctcattca ggttgatacg cttaccgagc
     9241 aacgttcggt tggcatcgaa cagtacagcc gttatgtacg taatcaactg aaattcctga
     9301 tacaggaaaa aatattcttc gacaaacaca cttcgagaat gtacaccaaa atcatgggta
     9361 tagctcccat gtatgcttta catcccgata acatggacag caaggaatca atgacctatt
     9421 tcagaaattc agttatttgc tggtttgctt acgatgaact gcgtccctat cttatcaaac
     9481 agtttgttgt gccaaaagga aatgataccg aaagaatgac ctttgatgat ttcttcagcc
     9541 agaagatgta tgcgagctac ctgctgggcg aaagtgatac ttacggacgt atgttgcttg
     9601 aatatgccgt agatgaggat gccctcaaag ccgaacagaa acgtattgaa acagaacttg
     9661 taaatttcga ggaagatctc tgggaatatt aagttcataa agtcaaataa tacgaagaaa
     9721 gacggcataa acagccgtct ttttttgtaa gcaaaaaata actaactttg cgacagcaat
     9781 ttaaaagtac aattcatgca agagattatt ctgtcagata atcaggatat tacccgggca
     9841 ggtttaatgc aaatcttctc ttcagaattt acgtgtcatt ttgtagaggc taaaaataaa
     9901 aaagagctta taaattgtct cgaaaattgt accgaagcaa tcgttgtgct tgattacacc
     9961 ctgtctgatt ttcagcgtgt cgaagacttg ctgatggtca gtgcccggtt tcaggatgca
    10021 ggatggctgc ttatatccaa tgaattgagg gatgaatttg taaggaacat atacctgaat
    10081 gccgagaatt tcagtatcgt ttataaggat tgcagcacgg aggagttact gatggcgatg
    10141 cgcgggttgc agaaggggca gcggtatata agtaacaccg tttcaaaaaa cttactggaa
    10201 agtgcattcc ggcaacaaac cggcgacagc aacaccaaac ttactgcaac cgaaaaggaa
    10261 attctgaaag atatagcatc cggaaaaacc acaaaggaaa tatctgatca gcggcatgta
    10321 agtacgcata ccatcgtcag tcaccggaag aatattttca gaaaaattgg ggtgaacaat
    10381 gtgcatgaag caacaaaata cgctgtaaaa gccggactta tcgatctggc agattatttt
    10441 atttaagaga attataacaa tgaatatagc gacattaatt tctggaggcg ttgacagttc
    10501 ggtagtggta catctactta aagagagcgg aataacacct tcgctgtttt atatcaagat
    10561 aggaatggac gatgatgagt tgcttcattg cagttcggag gaggatatag aaatggccag
    10621 tctggttgcc cgtaaatacg gatgtaagct ggaagtggtt gatttacata aggaatactg
    10681 ggataatgtg gtggcataca ccatcgacaa agccaaaaaa gggttgacac ccaatccgga
    10741 tgtcatgtgc aataagctga ttaagtttgg tgttttcgaa cagcgagtag ggatcagttt
    10801 cgacaagact gcaaccggtc attatgcgac gactattgaa aaggatggta aagtctttct
    10861 gggaacagcg aaagatcctg tgaaggatca gactgatttt ctggcacaga taaattacct
    10921 gcaggtatcc aaactgatgt tccccatcgg tcatctgatg aagagcgaag taagaaaaat
    10981 cgcggaaaac gtgcagcttc cctctgcaaa acgtcacgac agtcagggta tttgcttcct
    11041 gggtaaagtg aattataacg attttatccg gcggtatctc ggtgtgaagc aggggccaat
    11101 cgttgagctg gaaaccggaa gaattctggg gcatcacaac ggttactggt ttcatacggt
    11161 aggccagcgc aaaggccttg gactttcggg tgggccctgg tatgtgatcc ggaaagacgt
    11221 ggaatccaat attgtttggg cttcaaaagg ttttgaggtg gaatctcagt acggatatga
    11281 atttgcgatg aatgatttcc attttattac agagaatcca tggaaagaca cagaagaaag
    11341 tgttgatata acctttaaaa tcagacatac accggaattt accaaaggta agattattaa
    11401 gaccgctact ggttatcgca tagtttcaga agacaaactt caggggatag cagccggaca
    11461 atttggtgta gtgtatgacg aagcgtcagg tatctgcatt ggcagcggtg aaatcgtctg
    11521 acagcaggaa ttacatcccg ccaatccggc ataaattttc aatctgtttg ttccaaagca
    11581 gtatggcgtc ttctctctcg ccgggcgtcg cataatcggt aacaatcagt cctacatgcc
    11641 cgctcaggtc aagcgaaaca atctccagct gaaaataggc ttctgtgtct ttatcatcct
    11701 cccactggaa acagataagt tttccttcct ttacctgttt caggcaggca gtctgttcat
    11761 gaccttccca actgaaagtg tattcatttc cttcgacaat tactgcatct gaaaaccatt
    11821 cagccagccc gagaggtgtt cctatcattt tccagagaat agcttcagaa gcattattca
    11881 atggatattc gattgtaagt ttttcctttt tcatccgttc acgtgttggt tggttaatat
    11941 tgaacacaaa tacggcaagt ttcgcaatct gactctgcaa ctattcatat ccggaatccg
    12001 gcagcactct ccggttaaag tgttaaactg tctttcccga aactaagggg aagcaattgc
    12061 ttaatgctgt cgaacacata tattttatct gtaccgtaca gaattacttt tatcgcctgg
    12121 ccgtatctgt tttcagtctc cagcaaaacc tgccggcagg caccacaggg cgtaacaggc
    12181 tcagaggtga atttaccgtt ggtgaaagca gcgatggcca gtaacttaac aggaatgtca
    12241 ggatgttgag aattggcata gaaaatggct gtccgttcag cacaagttcc ggaaggatag
    12301 gccgaatttt cctgattgtt tcctccgaca acaatcccgt tttccagtaa tacagctgcg
    12361 cctacgtgaa attcagagta gggagcgtaa gatttctgag tttgtttctt tgcttcttca
    12421 attaatgaga tgtattctga aggaatttca tccggttgaa atactgaaat ttttatttcg
    12481 tgactgatat attgcatgaa cttgatattt gggttatggt acaaatatat aaaataattt
    12541 aactcaaacc gctatttcgt caataagttt tatatttgtg aaaaatttat caatcaaatt
    12601 caatccggtt tttctgcgat ttacgactat gaacagatcc ctcattttta tcatatcatt
    12661 gatatctttc tcccagactt ttctggctca gaacaggtcg gaatcgtacc tgaattatat
    12721 tgagaaatat tcaaaactgg cagttgagca acagaaacgg tatagaatac ccgcaagtat
    12781 caccctggca cagggcttac tggagtccgc agccgggatg agcgaacttt cccggacgtc
    12841 caacaatcat tttggcatca aatgtcacaa tggatgggaa ggtgaaaaag tatttcacaa
    12901 ggatgatcat gccgaaccgg attgtttcag aaaatataag gaggtaagcg aatcttacga
    12961 agaccattcg ttgtttttgt caaacagaca acattatgct tttctctttg acttgaatcc
    13021 aaccgattac aaagaatggg cttatggtct gaaaaaagcg ggctatgcct cggatcccgc
    13081 atatcccgtt aaactgatta aaatcattga agattatgaa ttacaccagt tcgatctcgg
    13141 cggaaaggtt gattatctcc actcaagaac cgaaaaggca gtaagaacac atgaatctaa
    13201 tagactaaaa acatctgccg gcattggcga tcatgattta ttccggaata ataaggtaat
    13261 ctgcattttc gctcacacca gcgacagctt tgaaagctta gccagtgaat ataatatccc
    13321 ggagaagaaa ctgcttcagt acaacgattt gagtgaaagc accgacattg tgcccggcac
    13381 cattatttac ctcaagacaa agaagaaaaa ggctgcagcc ggatttctgc agcatgtggt
    13441 gaaggaaggc gaaaatatgt accgtatttc ccaaaaatac ggtatccggc tggaaatgtt
    13501 gtacaagctg aacggtatgc catattcaga aggagccgag accggtatga ttctcaaact
    13561 acgataatcc gatgcgaaga aaaaatacgg aaaaactgag tgatattctc ggtgtagtac
    13621 tgaagcaaaa tcacctggat gaaaaactct acgaaacaag ggttctaaaa tcatggcctg
    13681 gtgttctggg tgataatgta atgaagtaca cgtccgatat ttatttcaac agaaagaaac
    13741 tttatgtaaa gttaacatcc tctgttctca gacaggaact tttcattacc cgcgaagaaa
    13801 ttcgaaattc actgaacaat tttgtcggtt ttccggttgt aaaagagatt gtgtttctgt
    13861 aataaacttt aatgcaaact tacactgctt ccgatggatt atgaagctgt aatatcacac
    13921 aggctaacga aggaagaagt actcatagta cgcgatgagt tgatttcaga aaaggaaagt
    13981 ttttctgatt tttttcagtt aatatttcag aaacctgtaa aaactgcctg gcaggttgcc
    14041 tggatatgtg agaaagtgag tgaagcggat gtgacgatgt ttaccgacga agattatcat
    14101 caactgatgg acttcacgct gaccaataat gaagacggtc tgcaacgact ctgtctttct
    14161 ataatactta acctgccttt gagaaaacca gtatctgttg aatttatcaa tctttgtttt
    14221 gatcagatga tttcgccccg caagcctgtt ggagtgcagg tactttcgat gaaaatctta
    14281 cgcaaaatct gtattgcagt gcctgatctt ggcgacgaac tgctcgtcat tcttgaaaat
    14341 accgacgatt tgacatatag tccgggattt atttcggccc gtaaacatgt gattaaatca
    14401 ttgaaaacta atagtaaagg tggatttggg gtaaacggaa ggttaaaaga ttatgcagaa
    14461 tggtaattta attgttacct ttgcacccga taaaaaattg ttttatttaa attaaaaagt
    14521 atgtctttaa agaaaaacat tatcgcttta cggcaaaaga aagctatcgt tgaaaaaggc
    14581 ggaggcgaca aagcccttga aaaacaaatg gctatgggta aaatgccagc tcgtgttcgt
    14641 attctggctt tactcgacga aggctcgttt catgaatatg acatgttcgt tgaacatgaa
    14701 gaacaaaatt tcggaatggc caacaaggag ttacctgccg aaggtgtaat tacaggaaca
    14761 ggtacaatta atggtgcccc tgtatgcatt tatgcacaag actttactgt aatgggcggt
    14821 tcgcttggtt tgaaacacgc ccgaaaaatt actaaaataa tggatcatgc cctgaaaatg
    14881 aagatcccct gcatcggtat caacgactcg gggggtgccc gtattcagga aggtatcggg
    14941 gcgcttgctg gttatggtga aatatttttc cgtaacacaa tggcttccgg agtaatacct
    15001 cagttatctg tcattcttgg tccctgcgcc ggtggtgccg tttattctcc tgcactcacc
    15061 gattttgtat ttgtggtaga gaatatctcc aaaatgttta ttaccggacc aaatgttatt
    15121 gaaaccgtac ttggtgaaaa aatttctcag gaagatctcg gaggtgcccg tgtacatgct
    15181 gaaattaccg gaaatgcaca cttctatgca acaagcgaaa tggagtgctt caaacagatt
    15241 aagaccttaa tttcactcat acctcacaat aatgaagtga aagctaagag tgtagcaccc
    15301 aaagatccca aatacagaaa gaaaatagaa aatattattc catctgatcc aaagcaaccc
    15361 tacgatgttc gtgaagtaat ctttgcgctc actgacgaat ccaaattctt tgaagtacag
    15421 gaactctggg cggccaatat tgttatcgga tttggacgta tgaatggtga aacagtagga
    15481 tttgtggcca accagccatt gtatcttgca ggtgtgctgg attgcgatgc ttccgataaa
    15541 gctgcacgtt ttatccgctt ctgcgatgcc ttcaacattc cgattataac gctggaagac
    15601 atgcccggat atctgcccgg agttgaccag gaacatgccg gtgtgatacg tcacggcgct
    15661 aaaatgctct atgcttattc cgaagctaca gttccaacaa tgaccatcat tctgcgaaaa
    15721 gcttacggag gaggttacat cgccatgaac tcacgtcacc tgggtgccga cttcgtttat
    15781 gcatggccaa gcgccgagat tgccgttatg ggtcccgaag gcgccgccaa tatcattttc
    15841 cgtaaggaaa ttatggaagc ggaagatgaa gatgcaatgc gtcaacagaa agtaaaggaa
    15901 tatgttgaga aatttgcaaa tccgtttgta gcagcctcca aaggttacat tgatgctgtt
    15961 atcgaaccgt ccgaaacccg tgatttgctg cttcatacgc tcgaagtttc cagactcaaa
    16021 gaagagcacc gtccgatgaa aaagcatgga atacctccat tctaatgaac aggtgttaat
    16081 gtttttaatt tttattatat ggcttaatat caatttatta ttagaaacat tatggctaaa
    16141 aaagaaaata acacagatag tacaggcaaa gctcagttga acgagcaaaa agctaacaga
    16201 aaacagacga tagaaagagc gaagaaaata caggctgccc gaaaagctga agaagctaaa
    16261 aaaaaggcgg ctgaaaagca gatagctcag aaacaaaaga aagaagaggt aaagccaacg
    16321 gttgctgccc cttcggtaaa tgttacggac gcagatgtta gaccaatgcc tgagaatgag
    16381 aaaaaaacta ttaatacaca ggtaaaaagt atgagtgcag aagaaaagaa ggagccggag
    16441 tacgttgatt ttgcaataac cgcccgaaaa tataaaacac tgctgacaaa caaatataaa
    16501 aaccggaaat tctggatcaa tccaagtccg tacgaaattc aatcgtatat tcccggcaca
    16561 ataatagaag tttgtgtgaa ggagggaaaa acagtaaagg aaggcgatcc gttgctggta
    16621 ctggaatcaa tgaaaatgca aaatcgcata gacatgccct ttacagctaa aataaaaaag
    16681 ataaacgtaa aagcggggga gagaatcccg aaagattttc tgatggtcga actcgaacaa
    16741 gcttaaaatc cggttaaata taaaacaggg gaagttgcag ctgcaatttc cccgtttttt
    16801 tttagtgatt gtaagttcgg cttaaaaaat cgtgctaaaa ctaatgctcc gcattacaca
    16861 aagcggagct ttgtctttaa ctatggcgga gcgaaaacgt tgatcaaaac attgtagctg
    16921 ttaaatggca cacaacgaag aa
//
LOCUS       DAOPYB010000052        16436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3740_length_16436_cov_9.502778, whole genome shotgun sequence.
ACCESSION   DAOPYB010000052 DAOPYB010000000
VERSION     DAOPYB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16436
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3740_length_16436_cov_9.502778"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /gene="prfB"
                     /locus_tag="PKH58_05790"
     CDS             join(<1..51,53..1111)
                     /gene="prfB"
                     /locus_tag="PKH58_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629026.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNX88572.1"
                     /translation="DQLKEVLEREDALRRYLDIDTKLIQIEEEELRTQTPDFWDDPKA
                     AEMQMRKVKELKKWIESYYEVKAAADELQLAFDFFREEAVTEEEVDDAYTHVTILLEA
                     IEMKNMLSNEEDSLGAVIKIVAGAGGTEAQDWADMLFRMYQRYCERQGYKCELTNYQG
                     GDEAGIKTVTIQVEGDMAYGYLKSENGVHRLVRVSPFNAQGKRMTSFASVFVVPLVDD
                     TINIEVNMANLSWDTFRSGGAGGQNVNKVETGVRLHYKYIDEQTGEEKEIAIENTEGR
                     TQLGNRENAMRLLRSQLYEMELEKRRAAQAVVEAGKKKIEWGSQIRSYVFDDRRVKDH
                     RTNYQTSDVTGVMDGKIEEFIKAYLMEFGREEKVG"
     gene            complement(1192..2388)
                     /locus_tag="PKH58_05795"
     CDS             complement(1192..2388)
                     /locus_tag="PKH58_05795"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNX88573.1"
                     /translation="MKILVLNCGSSSVKYKLLDMNSKEVLGSGGVEKIGMKGSFLKHT
                     KQNGEKVILEGEVLEHQLAIEYILGVLTSEKHGCIKNMEEIDAVGHRVVHGGEKFNSS
                     VLITDDVIRNIEACIEIAPLHNPPNLAGINAISDLLPQVPQVAVFDTAFHQTMPEYAY
                     MYGIPYSLYKKYGIRRYGFHGTSHRYVSKRACEFLGLDYKSTKIITAHIGNGGSITAI
                     ENGKSVDTSMGFTPVEGLMMGSRSGDVDLGVVTYLMEKEMISTGAATTLFNKHSGVLG
                     VSGISSDMRDIENAIAEGNQRAKLALEMYEYRIKKYIGSYAAALNGFDVLVFTGGVGE
                     NQTITRERVCKGLSFMGIEVDPEINKVRGEELLISTPNSRVKVVVIPTDEEYMIASDT
                     MELVGK"
     gene            complement(2405..3361)
                     /locus_tag="PKH58_05800"
     CDS             complement(2405..3361)
                     /locus_tag="PKH58_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNX88574.1"
                     /translation="MAEPIKEPIEKYGLSKRNHKKTLFTKIGVVGCGKDGSVIATAAA
                     SHGMEVIFLEPSEERIANAYARVEAKLDRKIKGWGLTESEKKATLSRIKGTMDYEDFH
                     GCDFVIEAIRYNNLTGERRVEQRKEVFKHLERVLSPQAIIASNVTTVIVSDLASDLEN
                     KSRCIGLHFLSNVPDSQMIEIVRGLETSDATYEKVCQFARLINYQFVPVVESAGLVSI
                     RLFLTQLNEACSILMENISNAEDIDHIMTIGFGHRQGLFRTADRLGIEKIVKLLENMY
                     DEYGHVKYKPSPVLLRMYRAKHFGISTKKGFYTYDDMGNIIK"
     gene            complement(3437..4438)
                     /gene="pta"
                     /locus_tag="PKH58_05805"
     CDS             complement(3437..4438)
                     /gene="pta"
                     /locus_tag="PKH58_05805"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNX88575.1"
                     /translation="MKLLEDIMQRAKENVQRIVLPEGTELRTLKAADFILKEKAAKLI
                     LIGDENEIKQLAVENNLQYISEAQIIDPETYPEMEKYSSLLYEIRKSKGMTPDEAVAL
                     AKDPLYLACLMIKNGDADGELAGARNTTGNVLRPAFQIVKTKPGIKIVSGALLMFTPK
                     PEYGENGLLVFADCAVNPNPTADELAQIAVSTAETAKVLGGFEPRVAMLSFSTKGSAQ
                     HELVDKVIEATRIAKEMAPEIQLDGEFQADAAIVPSVGQSKAPGNSIAGNANVLVFPD
                     LQAGNISYKLVERFSGAQAIGPVLQGIAAPINDLSRGCSVEDIIRMITITANLAMKK"
     gene            4718..6859
                     /locus_tag="PKH58_05810"
     CDS             4718..6859
                     /locus_tag="PKH58_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX88576.1"
                     /translation="MRTKKLLTAAVVIFTTISCNQQKVTNILKQTDFQKPPVADIKPE
                     TLTNFGQVRIDNYYWMKDKNNPKVINYLKTENAYTDTVMASTKDLQKKIYDEIIGHIK
                     EDDQSYPTLSNGYYYYTRTEKGKQYRTYCRKKGSEKAAEEIIFDVNKMAEGKPAYMFE
                     GYDISPDNRLAGYFFNETGSFADYILKIKDLTTGKDLDLTIKGASSFAWANDSKTLFY
                     TLIDPVTLRSYRVYRQVLGNPKANLIYEEKDAKFSLGVSDSKTKDYIFISINSTTTSE
                     ERFVSANKPTETFKVFMPRVKDVDYSVFTHKDYFFIRYKDKQNLNGKLYQVVVKDYAD
                     RKAWKEIMPHDKDVRIENLNVQKDYLIIETRKNGLANLEIRNLKTGKKEFINFPEPVY
                     TASSSGNPEYDAATFRYTYTSLNRPATLYEFNCQTSETKKLKQQEIPSGFNPDDYKVE
                     RLWANAEDGTKIPMAIVYKKNLKKKGKNPALLYSYGSYGMSSDVYFSGSFYSLIDRGF
                     VFGIAQIRGGSDMGEQWYEDGKLLKKKNTFTDFIACSEKLIADKYTSADKLAAMGGSA
                     GGLLMGAVANMRPDLYQTIVAQVPFVDVVTTMLDETLPLTTGEYEEWGNPNEEEYFRY
                     MLSYSPYDNIKAQNYPNMLVTGGINDSQVLFHEPSKYTAKLRALKTDDNILILHMNMD
                     SGHGGATGRYDSIKDTAFELAFILNRVGISK"
     gene            complement(6923..8995)
                     /locus_tag="PKH58_05815"
     CDS             complement(6923..8995)
                     /locus_tag="PKH58_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX88577.1"
                     /translation="MKLKKKTYIVSLICVLTIGVQAQDVRDNTEQIIADIYEQVSEDA
                     ETEIDFTSLYEDLIELAENPVNLNNTNKEELEKLQFLSDYQIQNILYYLYKCTPLNSI
                     YELQLIDGLDMTDIRHMLPFVSIGECESKFQKIYMRDLMRYGKNELYMRFDRGLETKE
                     GYRYLPEEDKETSNQNSKKYLGDPFYSYLKYRFHFRNRIQAGYTAEKDAGEQFWGNQH
                     KGYDFYSAYAELKNFGKMKTLVIGDYRANFGQGLVMRTDFSMGKSAYVLQVNPRSGGL
                     KKYSSTNESSFFRGGGITFKLGKTDLTAFYSNKMLDGDTINGSFPTILRDGLHRTNDN
                     LKTKHTVNEQVLGGNISFTHSWFQVGATLVHINLDKSLTPEKNIYNRFYFSGNKQTAG
                     SINYRFRWQKLNFSGETAIMDNYSLATVNSLNFSPVSRVGLVALYRYFSKDYDALYAN
                     TFSETSRVNNESGMYIGAEVKPVKFWKLSAYIDSYRFGWPKFGIYAPSIGKDMLIQAD
                     YAPKRNVAMLWRFKLEEKSHNFTDTLSTMPVVLSQPKWQARYQLNYSFGRFKFKNQLD
                     VNGFSDGVNTPGYGFSALQDVSYSFLQVPLTLDLRLHIFDAQNFENRIYTYEKDVLYA
                     FSIPMAYGQGTRYYLNMRYDVSRNLSLWIKLAQTVYADDRTTISSSNEEIQGNRKTDF
                     RFLLKWKF"
     gene            complement(9003..9824)
                     /locus_tag="PKH58_05820"
     CDS             complement(9003..9824)
                     /locus_tag="PKH58_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88578.1"
                     /translation="MKKICFALLSATCLINNINAQIAPVVPSSSSIADASVAVENDWS
                     AFRNTASLAYIDHIELSMQYENHSMIREMSWKSAQAGLNAKYFNIGLSFSYFGYSLYN
                     EMIAGVGFARNFSDKFSMGLQFNYYTAYFSAGEESRYRGALFPQAGISSHILPRLTIG
                     FQAFNPFQAIIKTENSQKRIPTIYSIGTNYEFSKNLVWLTQIDKEVSSDFRFATGFEY
                     TMLRQLTVKLGAYGSDYLVPSIGFGLHLDKLHFNLNGELHPLLGLNTLANLKYRF"
     gene            10048..10665
                     /locus_tag="PKH58_05825"
     CDS             10048..10665
                     /locus_tag="PKH58_05825"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNX88579.1"
                     /translation="MKKRLKISTIILLTFASVALYGQGNPSVPNGKFSFSTVMERVIA
                     DGDTLYVDYIREVYIYPPMVFTSKNQEKFYWRTVRDVKKALPYARLVSYEITHVNAEL
                     MKLSTDSERKKYISQYKKEAFRKYEKDLRKLTVNQGKMLMKLIDREYEVNTYDLVKGY
                     LGSVPAFFWNSVASLFGSTLRQDYDGKDKDRIVERVVTLVDAGQL"
     gene            10721..11329
                     /locus_tag="PKH58_05830"
     CDS             10721..11329
                     /locus_tag="PKH58_05830"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNX88580.1"
                     /translation="MVGFSIKRFFEKNFSFFQKQQRIKDKKYQIRADIRAKKRQLSEA
                     EKNEAAKLVFERIETMSAFQKAKVVFLYWSTSSELPTHEYIRKWSEKKIILLPSVSGE
                     RMHLRRYVSSKEMHKGEMNMVEPTTDIYKGHFDLAIIPGIAFDRERNRMGRGRGYYDR
                     FLHSKNVPVYGVGFDFQVLRKIPTTKKDMKVTCVFSPGKTIE"
     gene            complement(11357..12067)
                     /locus_tag="PKH58_05835"
     CDS             complement(11357..12067)
                     /locus_tag="PKH58_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88581.1"
                     /translation="MPYRRLPNTDQARLRALRTAILMGEKQSYDKQVVTFKTIHEAKT
                     FLNVFEKQMLLYKQTLESQINANKQYQQILYNARMYISHFIQVFNLSVIRGEIRKEHK
                     LFYQLDQDIHNVPDLSTEFAIQEWGKKIISGENERIRNGGSPIYNPTIAKVQVHYEVF
                     KEYKSTQRIYQSTTTHNWEELVNQRKKADNIILDIWNQVEEHYKSERPYSRLMKCKNF
                     GLVYYYRRNEKELTPEDD"
     gene            complement(12166..13143)
                     /locus_tag="PKH58_05840"
     CDS             complement(12166..13143)
                     /locus_tag="PKH58_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNX88582.1"
                     /translation="MYSFEETLQLIGSEIEHIDWDKEPRGLYQPIEYVLSLGGKRIRP
                     VVTLMSCNLYSDNVMQAMNSAIGLEIFHNFTLLHDDIMDRANIRRGKPTVHKKWDDNT
                     AILSGDVMQIEAYKWMTRSPETKLKQVLDLFSKTAAEICEGQQYDMEFEKRDNVSVDE
                     YIEMIRLKTAVLIAAGCQIGAWIGGADDEDAQNLYDFGNNIGLAFQLKDDLLDVYGNE
                     ANFGKKIGGDILCNKKTFLLIHALQIAEGSNAEELEYWLKNTNPEESCQKIKAVTELY
                     NRLSVKDICEEAINYYYNKAVASFSNVRIPDVKKSELRKFSEKLMSRND"
     gene            complement(13184..14158)
                     /locus_tag="PKH58_05845"
     CDS             complement(13184..14158)
                     /locus_tag="PKH58_05845"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNX88583.1"
                     /translation="MKRKIAIIAGGDSSEVIVSLRSAAGIYSFIDKEKYDCFIVTIVE
                     NNWQVEYSETEKLPIDKNDFSFLLNGKKILFDFAYITIHGTPGENGILQGYYDLIKMP
                     YSCCGVLAAAMTFSKFTCNQYLKGFGIRVADSILLRQGQKVSDEEVTEKIGLPCFIKP
                     NVGGSSFGVTKVKEQSQIQPAIEKAFSEGIEVLIETFTPGKEFTCGMYKTKTKTTVFP
                     VTEIVPKNEFFDYGAKYNNESEEITPARISENLTARIQKLTEAIYDILGCKGIIRVDY
                     IISEGEIINLLEVNTTPGMTVTSFIPQQVRAAGIDIRNVMTDIIEDSF"
     gene            complement(14231..15304)
                     /locus_tag="PKH58_05850"
     CDS             complement(14231..15304)
                     /locus_tag="PKH58_05850"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX88584.1"
                     /translation="MVEEEYSVDFEFPEDNDEPENEQEMYEHFRYVVDKGQSMLRIDK
                     YLVIQMASTSRNRIQDAADAGKILVNGKPVKSNYKVKPLDVISIVLAYPPSNFEIIPQ
                     DIPINIVYEDDSIMLVDKQPGLVVHPGFGNFDGTLLNAIAFHMKDHPDFDPNDSRIGL
                     VHRIDKDTSGLLLIAKTPDAKTALGKQFFEKSTKRTYNALVWGNVKEDEGTIIGSLAR
                     DPRDRMLFTVFKEGENPLAKHAVTHYKVLERLGYVTLVECRLETGRTHQIRVHMKHIG
                     HTLFNDDRYGGNEILKGNRTAKYQQFVHNCFEICPRQALHAKTLGFIHPVTGQEMYFE
                     SELPDDMRMLIEKWRRYSKQTEL"
     gene            complement(15314..16159)
                     /locus_tag="PKH58_05855"
     CDS             complement(15314..16159)
                     /locus_tag="PKH58_05855"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNX88585.1"
                     /translation="MEKNSFWNNTFVGYTLKNILIAIAVFIGLVWITLILIDFYTNHG
                     DTHKVPGLKGVSIEQAVKMLDKLDLKAEVIDSVYVKDQKLGTVIEQNPAEGSIVKPGR
                     PIYLIVNSRSVRLIALPQVADLSLRQAEATLQSVGIHVSSIQYAPSEYKDLVLGVKYR
                     GQNITRGTKIPEGSTVVLVAGNGYGAAATAVPAVMGMNLSTAIEIINASSFVVGGVIY
                     DLEPDGDEESYLIYDQRPAPGDSVSAGGTINLWLSKDPNKKAPPKVKKQQVEEEKVED
                     IEDFF"
BASE COUNT         4674 a         3357 c         3354 g         5051 t
ORIGIN      
        1 gaccaactta aagaagtgtt ggaacgcgag gacgcgttga ggaggtatct ttgacatcga
       61 taccaagtta atacaaatag aagaagaaga actgcgcaca cagacgcctg atttctggga
      121 cgatccgaaa gctgccgaga tgcagatgcg taaagtgaag gaactgaaaa aatggattga
      181 aagctactac gaagtgaaag ctgctgctga tgaactccag ctggcttttg actttttccg
      241 cgaagaagct gtcaccgaag aggaagtgga tgatgcatac acgcatgtta cgatattgct
      301 ggaagctatt gaaatgaaaa atatgctttc aaatgaggaa gattcacttg gagcagtgat
      361 taagattgtt gccggtgccg gtggaactga ggctcaggat tgggccgata tgcttttcag
      421 gatgtatcag cgttactgcg aaaggcaggg atacaaatgt gaattgacca actatcaggg
      481 aggtgatgag gctggtatta aaaccgtaac catccaggta gaaggtgata tggcttacgg
      541 ataccttaag agcgaaaacg gtgttcatcg tttggtgagg gtatcgccgt tcaatgcaca
      601 gggtaagaga atgacatctt ttgcttcagt cttcgttgtt ccactggtgg acgataccat
      661 caatattgaa gttaatatgg ctaatctgag ctgggataca ttccgttcgg gaggtgccgg
      721 tggtcagaat gtaaataagg tagaaaccgg tgttcgacta cattacaaat acattgatga
      781 gcaaacgggc gaagaaaaag aaatagccat tgaaaatacc gaaggtcgta ctcagctggg
      841 taaccgtgag aatgccatga ggcttttacg ttctcagttg tatgaaatgg aacttgaaaa
      901 acgccgggct gctcaggctg tggtggaagc cggtaagaaa aaaatcgagt ggggctcaca
      961 aatccggagt tatgtgtttg atgaccgacg cgtgaaggat caccgtacca actatcagac
     1021 atcggatgta accggagtga tggacgggaa aatcgaagaa ttcatcaaag cttatctgat
     1081 ggaatttggc cgggaagaga aagtcggtta attgaatata actcaataaa aaaacaacag
     1141 aggatgactg ataagctatc ctctgttatt ttttaaatat ttcgaaaaga tttattttcc
     1201 aacgagttcc atcgtgtccg aagcaatcat gtattcttca tcagtgggaa ttaccaccac
     1261 cttaaccctg gaattcggag tgctgatcag aagttcttca ccacggactt tgtttatttc
     1321 aggatcaact tcaataccca taaaactcag tcctttacag acccgttcac gggtaattgt
     1381 ctgattttca cccacacctc ccgtgaacac aagaacatca aaaccgttca gcgctgctgc
     1441 gtaagagcca atgtattttt taatacggta ttcatacatc tcaagcgcca gttttgcacg
     1501 ctgattgcct tcagctatcg cgttttcaat atctcgcata tcggatgaaa tacctgatac
     1561 tcccaatact ccgctgtgtt tattgaaaag tgtggtagca gcacctgtgc taatcatttc
     1621 tttttccatc agataggtta ctactccaag gtcaacatca cccgaacggc tccccatcat
     1681 cagtccttct accggtgtga aacccatgga tgtatccact gattttccgt tttcgatagc
     1741 agtaatggaa cctccattgc caatatgggc tgtgatgatt tttgtgcttt tataatcgag
     1801 gccgagaaat tcacaggctc gttttgacac ataccgatga cttgttccgt gaaatccgta
     1861 gcggcggatg ccatattttt tatacagtga ataaggaatt ccgtacatgt atgcatattc
     1921 gggcatagtc tgatggaacg ctgtatcgaa tactgccacc tgaggaacct gaggaagtaa
     1981 gtcgctgatg gcgttgatac cggcaagatt gggtggattg tgcaaaggag caatttcaat
     2041 acaagcttca atgttacgaa ttacatcatc agtaatgagt acgctcgagt tgaatttttc
     2101 accaccgtgt accacgcggt gtccaacggc gtcaatttct tccatatttt taatgcagcc
     2161 atgtttttcg cttgtgagta ctcccaggat atattcaata gccagctgat gttcaagtac
     2221 ttcaccttcg agaattactt tttctccatt ttgcttggta tgtttgagga atgagccttt
     2281 catacctatt ttttccacac ctcccgaacc aagaacttcc ttgctgttca tgtccagcag
     2341 tttatatttt accgacgaac tgccacagtt taatactaat attttcataa ctttctgtcc
     2401 tccattattt tataatattt cccatgtcgt cgtaggtata aaaccctttt ttggtgctta
     2461 tcccgaaatg ttttgcgcga tacatccgta gtaaaacagg agaaggtttg tattttacat
     2521 gcccgtattc atcatacata ttttcaagca gtttcacgat tttttctatt ccgagccggt
     2581 cagctgttct gaatagtccc tgccggtgtc caaatccaat tgtcatgata tggtcaatat
     2641 cttcagcatt tgatatattt tccatcagga tagagcatgc ttcgttgagc tgtgtcagga
     2701 aaaggcgaat gctcacaagt cctgccgatt cgacaacggg gacaaactga taattgatca
     2761 gtcgggcaaa ctgacatact ttttcgtaag ttgcgtccga tgtttccaga ccccgtacaa
     2821 tttctatcat ttgcgaatcc ggaacatttg acagaaaatg cagtccaata cagcggcttt
     2881 tattttcaag atcagatgcc aggtctgata caatgacggt agttacgttc gaagctataa
     2941 tagcctgagg tgacaaaact ctttccaggt gtttgaatac ttcttttcgt tgttctactc
     3001 tgcgttcacc ggtaaggtta ttgtaacgaa tagcttcaat tacaaaatca caaccgtgaa
     3061 aatcttcgta gtccatggtt cctttgatac gactcaacgt ggcttttttc tcgctttcgg
     3121 ttaaccccca acctttaatt ttgcggtcca gttttgcttc aacgcgggcg taagcattgg
     3181 caattctttc ttccgagggt tccagaaaaa tcacttccat tccgtgtgaa gctgctgctg
     3241 tggctatcac actaccatct tttccgcaac caaccactcc gatttttgta aaaagtgttt
     3301 ttttgtgatt gcgtttgctt aaaccatatt tttcaatagg ttcttttatt ggttctgcca
     3361 ttttttattc taattattga atttgttcta tagcaataaa aattgaaatc tcgttatata
     3421 ctgattgaca ggctgcttat tttttcatag ccagattggc cgtgatcgta atcattcgaa
     3481 tgatatcctc caccgaacat ccgcgcgaaa ggtcattaat cggcgctgca ataccctgta
     3541 gtacaggacc gattgcctga gcgcctgaaa aacgttctac cagtttatag ctgatatttc
     3601 cggcttgcag gtcggggaat accagtacat tggcattgcc tgcaatggaa ttaccgggcg
     3661 ctttgctttg tccgacagaa ggaactattg ctgcatcagc ctgaaattct ccgtcgagct
     3721 gaatttcggg cgccatttct ttggctatcc gggtagcttc aatcacttta tcaaccagtt
     3781 cgtgctgggc gctgccttta gttgaaaaac taagcattgc tacgcgtggt tcaaacccac
     3841 ccagaacttt ggctgtttca gctgtgctga cagcaatctg tgccagttca tcggcagtag
     3901 gatttggatt gactgcacag tcggcaaata cgagtaatcc gttttcgcca tattccggtt
     3961 tgggagtgaa catgagcagc gcccctgaaa ctatttttat acccggtttt gtttttacaa
     4021 tctgaaatgc gggccgtagt acattacctg ttgtgttgcg ggctcctgca agttctccgt
     4081 cagcatcccc gtttttaatc atcagacaag ccagatatag tggatctttc gccagtgcaa
     4141 cagcttcgtc tggtgtcatt cctttcgatt tgcggatttc atacagcagg cttgaatact
     4201 tctccatttc cggataagtt tccggatcaa taatctgagc ctcgctgata tattgaaggt
     4261 tgttttcgac tgctaattgt ttaatttcat tttcatctcc gataagtata agcttagcgg
     4321 ctttttcttt caggataaaa tcggctgctt tgagtgtacg caattcggtt ccctcgggca
     4381 gtacaatgcg ttgtacgttt tctttggccc gttgcatgat atcttctaat agtttcatag
     4441 ctgaaatgta ttttgttgtt aatcagttga tgtctgaagg actgaattag tttgaaattc
     4501 agcgaattag gcatttgctc ctgcattgaa gcaatggatt attgtgtgcc tgcaaaggta
     4561 caatgttttt gcgaaattgc aatgtagagt ttgcaatttt ttgaaatata gtgtattgtt
     4621 atacgcgata ttaattgtaa ttttggaacc gataaacttt catattttgc aggtaattat
     4681 tttcattacg cgttttataa cctgaaaaac cagaatcatg agaacaaaaa aattattaac
     4741 cgcagcggta gtgattttta ccaccatttc gtgcaaccaa caaaaagtta caaatatttt
     4801 gaaacagaca gatttccaga agccccctgt ggccgatatt aaacccgaaa cactcacaaa
     4861 tttcgggcaa gtgagaatag ataactatta ctggatgaag gataaaaaca atccgaaagt
     4921 gataaattat ttgaaaacag agaatgccta taccgatact gtgatggcat cgaccaaaga
     4981 tctgcagaag aaaatttacg atgagattat cggacacatc aaggaagacg accagtcgta
     5041 tcccacgttg tcaaatggct attattacta tacccgtacc gaaaaaggca aacaataccg
     5101 cacctactgc cgcaaaaaag gatcggaaaa agctgctgaa gaaattatct ttgatgtcaa
     5161 caaaatggcc gaaggtaaac ctgcatatat gtttgaaggg tacgatatta gtcccgacaa
     5221 cagactggcc ggttattttt tcaacgaaac aggttcattt gccgattata tcctgaaaat
     5281 caaagatctg acaaccggaa aagatttgga cctaaccatc aaaggtgcat cctcctttgc
     5341 ctgggctaac gatagtaaaa cactgtttta caccctgatt gatcctgtaa cattacgatc
     5401 gtaccgcgtt taccggcagg tgctgggcaa cccgaaagct aacctgattt acgaagaaaa
     5461 agatgcaaaa ttctccctgg gtgtttccga ttccaaaaca aaagattaca tattcatcag
     5521 tatcaacagt acaaccactt ctgaagagcg atttgtttct gccaataaac ccacagaaac
     5581 attcaaagtc tttatgccac gtgtcaaaga tgtggattat tctgttttta cacataaaga
     5641 ctactttttt atccggtaca aggacaaaca aaatctgaat gggaaactct atcaggttgt
     5701 tgtgaaagac tatgccgacc gtaaagcctg gaaagaaata atgcctcatg ataaggatgt
     5761 gcggattgaa aatttgaacg tgcaaaagga ttatctgatt attgaaaccc gtaaaaacgg
     5821 tctggcaaat ctcgaaatcc gaaatctgaa aaccggaaag aaggaattta tcaattttcc
     5881 cgaacctgtg tacacggctt cttccagtgg aaatcccgaa tacgacgcag caactttccg
     5941 ttacacctat acttcgctca accgtcctgc cactttatac gaattcaact gtcagacttc
     6001 cgaaaccaaa aaactgaagc aacaggaaat tccttccggt ttcaaccccg atgactataa
     6061 ggtggaaagg ctgtgggcta atgcagaaga tggaacgaaa atcccgatgg cgattgttta
     6121 caaaaaaaat ctgaagaaaa aaggtaaaaa tccagctttg ctttattcgt acggaagtta
     6181 tggcatgagc tcagatgttt atttcagcgg cagtttctat agtctgattg atcgtggctt
     6241 tgtgtttggc attgcacaga tacgaggagg cagcgatatg ggcgaacagt ggtatgagga
     6301 cggaaaactg ctgaaaaaga aaaatacatt tactgatttt atcgcctgca gcgaaaaact
     6361 gattgccgat aaatatactt ctgccgacaa acttgccgcg atgggtggca gcgcgggagg
     6421 cttgctgatg ggagcagtag ctaatatgcg acccgatttg tatcagacta ttgttgctca
     6481 ggtacctttt gtcgatgtgg taactaccat gctcgacgaa acacttccac tcactaccgg
     6541 cgagtacgaa gaatggggaa atcccaacga agaagaatat ttccggtata tgctttctta
     6601 ctccccctac gataatatca aagctcagaa ttatccgaat atgctcgtta caggaggtat
     6661 taacgattca caggttttat tccacgaacc aagtaaatac acggcaaaac ttcgcgcact
     6721 caaaacagac gataatatac tgattttaca tatgaacatg gattctggtc atggtggcgc
     6781 tacaggccgt tatgacagca ttaaggacac tgctttcgaa ctggcattta tactgaaccg
     6841 ggtgggtatc agtaaataaa ttcttcaatt gacaacagaa acatcagagt attaactgtc
     6901 tgtgtttctg ttgttcatta ttttaaaatt tccatttaag taaaaaccga aaatcggttt
     6961 tccggttgcc ctgaatttct tcgttgctgc tgctgattgt tgtgcggtcg tcggcatata
     7021 ctgtttgagc cagcttgatc catagtgaaa gattgcggct gacatcgtaa cgcatattga
     7081 ggtaatagcg ggttccctgc ccgtatgcca tcgggatgga gaaagcataa agcacatctt
     7141 tttcgtaagt gtaaatccgg ttttcaaaat tctgagcatc aaaaatatgt aatctcaggt
     7201 cgagggttaa cggtacttgc agaaaactat aactcacgtc ctgcagtgct gaaaatccat
     7261 atccgggtgt atttactcca tcgctgaatc cgttaacatc cagttgattt ttgaatttga
     7321 accgtccgaa cgaataattc agctggtaac gagcctgcca ttttggctgt gatagtacaa
     7381 ccggcatggt gcttaaagtg tccgtaaaat tatgactttt ttcttcgagt ttgaaacgcc
     7441 atagcatcgc cacgtttcgc ttcggagcgt aatcagcctg tatcagcatg tcttttccga
     7501 tggatggagc ataaatgcca aattttggcc atccgaaacg atagctgtct atgtaagcgg
     7561 ataatttcca gaattttaca ggcttgactt ctgctcctat atacatgccg ctttcattat
     7621 ttacccggga tgtttctgaa aaagtattgg catataacgc gtcataatct ttagaaaaat
     7681 accggtacaa agctaccaga cctacccggg ataccggaga gaaattcaga ctgttgacgg
     7741 ttgcaagcga atagttatcc ataatggctg tttcgcctga aaaattcagt ttttgccatc
     7801 taaaccggta gtttatactt cctgctgttt gtttattgcc tgaaaaataa aaacggttat
     7861 agatattttt ctcgggcgtc aagcttttat ccagattgat atgaaccagc gtagctccaa
     7921 cctgaaacca ggaatgtgtg aagctgatat taccacccaa aacttgctca tttactgtgt
     7981 gtttggtttt cagattgtcg tttgtgcggt gcagtccgtc gcgaagtatt gtcggaaaac
     8041 taccgtttat cgtatcgccg tcaagcattt tattagagta aaaagcagtc agatcggttt
     8101 ttcctagttt gaaagtgatg ccacctcctc tgaaaaaact ggattcattt gtcgaactgt
     8161 attttttcaa accacccgaa cgtggattga cctgtaacac ataagctgat ttacccattg
     8221 agaaatcggt acgcattacc agtccctgcc cgaaattagc tctgtagtca ccaataacca
     8281 gtgttttcat tttgccaaaa tttttcagtt cggcgtaagc tgagtaaaaa tcataacctt
     8341 tgtgttggtt gccccaaaac tgttccccgg catctttttc tgccgtatat ccggcttgaa
     8401 ttcgattacg gaagtgaaag cgatatttca ggtaagaata gaacggatct cccagatatt
     8461 tctttgaatt ttgattgctt gtctctttgt cttcttcggg caagtatcta tatccctctt
     8521 ttgtttccag accccggtca aatcgcatat atagttcgtt tttgccatac cgcattaaat
     8581 ctctcatgta tattttctgg aacttgctct cacattcacc aatacttaca aacggtaaca
     8641 tatgacggat atctgtcatg tccagaccat caatcaactg cagttcgtat atggagttta
     8701 atggagtaca tttgtagaga tagtaaagta tgttttgaat ctgatagtcg gaaagaaact
     8761 gaagtttttc aagttcttcc ttgttcgtgt tgttcaggtt aaccggattt tcagcaagtt
     8821 caatcaaatc ttcataaaga ctggtgaagt caatttccgt ttcagcatct tccgaaacct
     8881 gttcgtatat gtcggcaatt atttgctcgg tgttgtcacg cacatcctga gcctgcactc
     8941 caattgtcag tacacaaatc aatgagacta tatatgtttt tttcttcagt ttcatagcct
     9001 aatcagaaac gatatttcag atttgccagt gtattcagac ccagaagcgg atgaagttcg
     9061 ccgttgaggt taaaatgtaa tttgtccagg tgtaaaccaa atccgatgct gggcaccagg
     9121 taatcactac cgtaagctcc aagtttgaca gtaagctgcc ggagcatagt atattcaaac
     9181 cccgtggcaa aacgaaaatc gctgctcact tccttgtcaa tctgagtaag ccatacaaga
     9241 tttttggaaa attcgtaatt agtacctata ctatatatgg taggaattct tttttgcgaa
     9301 ttttcggttt taataattgc ttgaaaagga ttgaaagcct gaaaaccaat agtcagacga
     9361 ggaagaatgt gtgatgaaat gcctgcctga gggaacaatg cacctctata tcggctttcc
     9421 tcacctgcgg agaaataggc tgtataataa ttaaactgaa gacccattga aaatttgtcg
     9481 ctgaaattac gggcaaatcc gacaccggca atcatctcat tgtaaagcga atagccaaaa
     9541 taagagaacg aaagacctat attgaagtat tttgcgttta accctgcctg agcgctcttc
     9601 cagctcattt cccggatcat ggagtggttt tcatactgca tggaaagctc aatatgatcg
     9661 atataggcca gcgaggcagt attccgaaag gcgctccagt cattttcaac ggctacagaa
     9721 gcatcagcta tagaagaaga tgacggaaca accggcgcta tttgagcgtt tatattattg
     9781 ataaggcaag tggctgacag taaagcaaaa caaatctttt tcatgaattt aacagattat
     9841 tgtatgatgt gcgagtcgac catgcgggtt caggcaatat aatcgacacg ttaaaaataa
     9901 gagaattttc aaaaatataa aaataaatca aaaaaatgtt tatacttcga cgaaataatt
     9961 tggaataaaa tttgttattt agttgtagaa cttgtaaata tcagttactt ttgcatcgct
    10021 aaatatatga aactaaacac tacagtcttg aaaaaaagat tgaaaatatc aactattatc
    10081 ctgcttacat ttgcctcagt ggcactttat ggtcagggta atccctctgt tccgaatgga
    10141 aaattttcct tttcgaccgt aatggagaga gtgattgctg acggagatac tctttatgta
    10201 gattatatcc gcgaggttta tatatatcct cccatggttt ttaccagcaa aaatcaggaa
    10261 aaattttact ggcgaactgt tcgtgatgtc aagaaggctc ttccttatgc ccggttggtt
    10321 tcgtacgaga taacacatgt aaacgcggaa ctgatgaaat tgtcgacaga cagtgaaaga
    10381 aagaaatata tcagtcagta taaaaaagaa gctttcagaa agtatgaaaa agatctgcgt
    10441 aagctgactg ttaatcaggg gaaaatgctg atgaaactca ttgatcggga atatgaagtg
    10501 aatacttacg acctggttaa gggctatctg gggagtgttc ccgcattttt ctggaattct
    10561 gttgccagtt tgtttggatc tactctgagg caggattacg atggtaagga taaagaccga
    10621 attgtggagc gcgtggttac actggttgat gccggtcagt tatagctatc tcatgaaagt
    10681 gtcttgtatt ataaatgaac ttttttgaaa atatattgat atggtgggat tttcaataaa
    10741 gcgttttttt gaaaaaaatt tcagtttttt tcagaagcaa caacggatta aagataaaaa
    10801 atatcagatt cgtgctgata tcagagcaaa aaaaagacag ttatcagaag ctgaaaaaaa
    10861 cgaagcagcc aaattggttt ttgaaaggat tgaaaccatg agcgcttttc aaaaagctaa
    10921 agttgtattt ctctattggt caacatcttc cgaattacca acccatgaat atataagaaa
    10981 gtggagtgaa aaaaagatta ttttgctccc ttccgtttcc ggcgaaagaa tgcatcttcg
    11041 cagatacgta tcatcaaaag aaatgcataa aggtgaaatg aatatggtgg aacctactac
    11101 cgatatttac aaaggtcatt tcgatctggc tattataccc ggtattgctt ttgaccgtga
    11161 aagaaaccgt atgggccggg gcagaggata ttacgaccgg ttcctccact caaaaaatgt
    11221 tccggtttac ggtgtgggtt ttgattttca ggtattgaga aaaataccga ctacaaagaa
    11281 ggatatgaaa gttacctgtg tttttagtcc gggtaaaacc atcgaataat caattgcctg
    11341 acaaatcgaa ttgtaattaa tcatcttccg gcgtaagttc cttctcgttg cggcgataat
    11401 aataaaccaa tccaaaattt ttacatttca ttagtctcga atagggtctt tcggacttat
    11461 aatgctcttc tacctgattc cagatatcaa ggatgatatt atctgctttt tttcgctgat
    11521 tgacaagttc ttcccagtta tgtgtcgttg tgctttggta tattcgctgg gtggatttgt
    11581 attccttaaa aacctcgtaa tgaacctgga ctttagcaat cgtaggatta taaatgggtg
    11641 atccaccatt tcttatccgt tcattttcac ctgatattat ttttttcccc cattcctgaa
    11701 tagcaaactc ggtactcaaa tcgggtacat tgtgtatatc ctgatcgagc tgataaaaca
    11761 gtttatgttc ttttcttatt tctccgcgaa tgaccgatag gttgaaaacc tgaataaaat
    11821 gagagatata catccttgca ttgtacaata tctgttgata ctgcttgttt gcatttattt
    11881 ggctttcaag agtttgcttg tatagaagca tctgcttttc aaacacattc aggaaagttt
    11941 ttgcttcatg gatagtcttg aatgtaacca cttgcttgtc gtaactttgc ttttctccca
    12001 tcagtatggc tgttcgcaaa gctcttaatc gagcctgatc ggtatttggc aagcggcggt
    12061 aaggcatgga tatactttaa attgtatttt ttctctggtt gtgtaatcgt tggagaaatg
    12121 ttaaatcata gataaattgg tctccttcag caaaagtaca attttttaat cgtttcggga
    12181 catgagtttt tcggaaaact tccttaattc ggatttttta acgtcaggaa tacgaacatt
    12241 actgaacgat gcgactgctt tgttgtagta ataattaatg gcttcttcgc aaatatcttt
    12301 gactgatagt ctgttataca gttcggttac tgctttgatt ttttggcacg attcttcagg
    12361 attggtgttc tttaaccaat actccagttc ttcggcatta cttccttcag caatttgcag
    12421 ggcatgaatc aacagaaatg ttttcttatt acacaaaata tcgcccccta ttttctttcc
    12481 aaaatttgct tcattaccat atacatccag caaatcgtct ttcagttgaa aagctaaacc
    12541 aatgttgttg ccgaaatcat aaaggttttg agcatcttca tcatctgctc ctccgatcca
    12601 tgcccctatc tggcagccag ccgcaatcag tacagccgtt ttcagtctga tcatctctat
    12661 gtattcatcc accgaaacat tatcacgttt ttcaaattcc atatcatatt gctgaccttc
    12721 acaaatctca gctgcagttt tggaaaacag atccagaact tgtttcagct tggtttccgg
    12781 tgatcgggtc atccatttat atgcttctat ctgcatgaca tcacccgaaa gaatggcggt
    12841 attatcgtcc cattttttat gtacggtggg ttttcctcta cgtatatttg cacgatccat
    12901 aatgtcgtcg tgcagtagtg tgaaattatg aaatatctcc agtccgatgg ccgaattcat
    12961 tgcttgcatg acattgtcag agtacagatt gcatgacatg agagttacta ctggtcttat
    13021 cctttttcct cccaatgaaa gtacatattc tatcggctga taaagcccac ggggttcttt
    13081 atcccaatcg atgtgttcga tttctgaacc aataagctgc agtgtttctt caaatgaata
    13141 catatcggtt gattttgcag aattaaaatt attctttctg aatttagaaa ctatcttcga
    13201 taatatcggt catcacgtta cgaatatcga taccggctgc ccgcacctgt tggggaatga
    13261 aactggtaac agtcattccc ggtgtggtgt tcacttccag cagattaatg atttctcctt
    13321 ccgaaataat ataatcgaca cggataatcc ctttgcatcc taaaatatca taaatggctt
    13381 cggtcagttt ctgaattcgt gcagtaaggt tttccgaaat gcgtgccggg gtaatttctt
    13441 ccgattcgtt gttatatttt gctccgtaat cgaagaactc gttttttgga acaatttcag
    13501 taaccggaaa aacagttgtt ttggttttag tcttgtacat gccgcaggta aattccttgc
    13561 cgggtgtgaa tgtttcaatc agaacttcga taccttccga aaatgctttt tctatggctg
    13621 gttgtatttg cgattgttct ttaacttttg tcactccaaa actgcttccg cctacgttgg
    13681 gtttaatgaa acagggcaaa ccgatttttt cagtcacttc ttcgtcgctc actttctggc
    13741 cctgacgcaa caatatcgaa tcagcaaccc gaatgccaaa tcctttcaga tactgattgc
    13801 aggtaaactt gctgaaggtc atggcggctg caagtacacc acagcaggaa taaggcattt
    13861 taatcagatc ataatagcct tgcagaatgc cattttcgcc gggagtgcca tgaatggtga
    13921 tgtaggcaaa gtcaaacagt atttttttgc cattcagcag aaagctgaaa tcatttttat
    13981 caatcgggag tttttctgtt tcactgtatt caacctgcca gttgttctcc acgattgtga
    14041 cgatgaagca gtcatatttt tctttatcga taaaagaata aataccggct gcacttcgga
    14101 gcgatacaat cacttcggac gaatcgccac cggcaataat tgcaatcttt cttttcatgt
    14161 gaaattcttt ttttatgatt gaaagcagtc agcgtgcaaa aaacacggct tgtcgcatct
    14221 taataattat ttataattca gtttgttttg aatatcttct ccatttttct attagcattc
    14281 gcatatcgtc aggcagttcg ctttcgaagt acatttcctg accagttacg ggatgaataa
    14341 atcccagtgt tttggcatgc aaggcctgac ggggacatat ttcaaaacag ttgtggacaa
    14401 actgctgata ttttgccgtt cggtttcctt tcagtatttc attgcctccg taccggtcgt
    14461 cgttgaagag tgtatgacca atgtgtttca tgtggacacg tatctgatga gtacggccgg
    14521 tttccagccg gcattcaacc aacgtaacgt aacccagtct ttctaatacc ttatagtgtg
    14581 tgactgcatg ttttgccaga ggattctcac cttctttgaa aacggtgaaa agcattctgt
    14641 cgcgcggatc acgtgcgagg ctaccgatta ttgttccttc gtcttctttc acattgcccc
    14701 acactagcgc gttgtacgtc cgttttgtcg atttttcaaa aaactgtttt ccaagtgccg
    14761 ttttggcatc cggagtttta gctatcagca gcaaaccgga agtatccttg tcaatccggt
    14821 gcaccagacc aatacgcgaa tcgttcggat cgaagtccgg atgatccttc atgtgaaagg
    14881 caatggcatt gaggagtgtt ccgtcaaaat taccgaaacc gggatgaaca accagtcccg
    14941 gctgtttatc caccagcatg atggagtcat cttcatacac gatatttata ggaatatcct
    15001 gcggtatgat ttcaaaattt gaaggaggat aagcaagtac aatggatatc acatccagtg
    15061 gttttacctt gtaatttgat ttaaccggct tgccgtttac cagtattttt ccggcatctg
    15121 cagcatcctg aatccggttt cgcgaagtgg aagccatctg aataaccagg tatttgtcga
    15181 tgcggagcat ggattgtcct ttatccacca cgtagcggaa atgttcatac atttcctgct
    15241 cattttcagg ctcatcgttg tcttccggaa attcaaaatc cacagaatat tcttcttcaa
    15301 ccatattcat cgcttaaaag aaatcttcaa tatcctccac tttttcttct tccacctgtt
    15361 gtttttttac tttcggggga gcttttttgt tcgggtcttt gctcagccat aagttaatag
    15421 tgccgcctgc tgaaacggag tcacccggag ccggacgctg atcgtaaatc agataacttt
    15481 cttcgtcgcc atcgggttcc aaatcgtaaa taacacctcc taccacaaac gaggaagcgt
    15541 tgatgatttc aatggcagtt gataagttca tacccatgac cgcaggaacg gctgttgctg
    15601 cagcaccgta accgttgccg gcaaccagta ccaccgtact tccttcggga atttttgtac
    15661 ccctcgtaat gttttgtccg cggtatttta cccccagaac caggtctttg tattcggaag
    15721 gggcatattg gatggaactg acatgaatac caacggactg caaggtagct tcagcctgac
    15781 ggagcgagag gtcggcaacc tgaggaagcg cgattagcct gaccgaacgc gaattgacaa
    15841 tcagataaat cggtctgccg ggttttacaa tagaaccttc tgccgggttt tgctcgataa
    15901 cagttcccag tttctgatct ttgacataca cagaatcgat gacttcagct tttaaatcaa
    15961 gtttgtcgag cattttcact gcctgttcga tgcttacacc tttcagtcct ggaactttgt
    16021 gtgtatcacc gtggttggtg taaaaatcaa tcagtatcag agtgatccat actaatccga
    16081 tgaatacagc gatggcaatc aatatgtttt ttaatgtata accgacaaat gtgttattcc
    16141 aaaagctatt tttctccata aattctgagg attaaacaga taaaatctat caggatcaaa
    16201 aattttttaa tttaaaatgc aaaaatactg aaattattgc actatttatc cgtttatcaa
    16261 aagaaatatc ggtccttcag ttttcaccgg ccgggtaaat tctctttttt tgaaattatc
    16321 ccgaacggaa aaattagaaa ttttaaagaa taaattaaaa gcctttcgca atttaaatcg
    16381 tacttttgca agttaataga aatttcattc aatacatctg caagaagatt attaaa
//
LOCUS       DAOPYB010000053        16314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3775_length_16314_cov_9.124915, whole genome shotgun sequence.
ACCESSION   DAOPYB010000053 DAOPYB010000000
VERSION     DAOPYB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16314
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3775_length_16314_cov_9.124915"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKH58_05860"
     CDS             complement(<1..443)
                     /locus_tag="PKH58_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX88586.1"
                     /translation="MKNTKVKLLLMISALLFVGFANAKSNKSVTFPYSAKTELGTENA
                     QQIPDQQKVKVSGQVKDVSGEPVIGVSVVEQGTANGTATDINGNYSLNVSGNGKLTFT
                     YVGFVSQTISVEGKSLINLTMSEDTKTLDELVVIGYGIVKKSDLTG"
     gene            complement(520..1296)
                     /locus_tag="PKH58_05865"
     CDS             complement(520..1296)
                     /locus_tag="PKH58_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HNX88587.1"
                     /translation="MKVKNFLLIILAFVFVSMAPPKVKILMAGDSTMADKPLTKHLTD
                     TLTGETFDEPLLERGWGQLFPEFVNNKAVVVNYAKNGRSTRTFIEEGLWTELIKNVRK
                     GDIVIIQFGHNDEVETKKSYTKPEQFRLNFIAFVNDVRAKGGNPILCTAVARRSFDEK
                     GNLLPTHGEYPSYIRQVAAEQKVPLIDMEKLTSEWLQKEGVENSRKYFHKIPPGVSRV
                     YPKGLDDNTHFNEAGARQVTKMFVSQVKEKKIKPLTKLIK"
     gene            complement(1297..2871)
                     /locus_tag="PKH58_05870"
     CDS             complement(1297..2871)
                     /locus_tag="PKH58_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HNX88588.1"
                     /translation="MTLGMSLQAKNTYEYLYKDLPFTMPKLKAPVFKNNRISVADCGG
                     VGDGMVLNTNAFAKAMETLSEKGGGTLVVPKGIWFTGPIVFRSNINMYLEKGAVILFS
                     PDFNLYPIVETIFEGLDTRRCQSPISGRNLVNIAITGKGSINGFGEAWRPLKKNKVTA
                     SQWKKVTSSGGVVKNDTWYPSESSVQGAEISDMNVPRQNLTEQEWLSIKDFLRPVMVS
                     FIECKNVYFQGVLFENSPSWNLHPLMCENVILDGLFVRNPGYSQNGDGLDLESCRNSI
                     IVNCQFDVGDDGICIKSGKDKPGRVRGRPTENVIVDNCKVFQGHGGFVVGSEMSGGVR
                     NISVKNCQFLGTDVGLRFKSTRGRGGVVENIYISDIYMFNIETESFLFDLYYGGKSAT
                     ETLEDGDKVSDNEINYPVNEETPVFRNIFVKNLISRNARRAMFFNGLPEMKISNIHIE
                     DVKITAQLGAELSNSKDIIFKNVHIVPQTGSALILKEVENFNSDNFTYPAGLEKPVDI
                     QGNGNNNIVISGKMIK"
     gene            complement(3023..4654)
                     /locus_tag="PKH58_05875"
     CDS             complement(3023..4654)
                     /locus_tag="PKH58_05875"
                     /inference="COORDINATES: protein motif:HMM:NF027642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5123 domain-containing protein"
                     /protein_id="HNX88589.1"
                     /translation="MKRNKNIFAAIVVILLMVSGTFSACTEDEPELQLPYLFRPINFQ
                     VELNKTIATITWSPVDSAVSYTLQLSNDSLDYSSPILDITTTKLSVVKELAGDTKFYA
                     RIRANSGIESKSSKFNTTLSFKTPKENLFQGYGTGNNTGVLYSAYMTATKTLTVKWLP
                     GANTTHLILTADGGLKDSVLISASEAAAGSKIVSLPANALWKTRIYNGKILRGTTYGL
                     TEGDIYLSAGEDLLSAMTNATSGQVILLAGGATFPIGGSAYNFNKNLKIRSASPVNRA
                     VICMTSGTPTSTANMFGVEASAAIDSLVFENIDFTGYCDNNPAATKIGYLFNNKTAYA
                     IASLKFINCNIHNIGNTPMRMSGNTEQRISDLKFKGCVINDVGFSSTYAIVNSNSKDY
                     FDNITFEYCTVYNFKGSLILRTGAYTMGTVSLTNCTFNQATQDAGSTRLLVDLNNMTV
                     TGGLTIKNCIFGSNGTIAAGVRTTGTKTISGTYFTTDYLDETLVGGATYTIKDKLTSF
                     NGASTDLWNNPVNGDFTLKTTSFAGKGISGDLRWY"
     gene            complement(4693..6543)
                     /locus_tag="PKH58_05880"
     CDS             complement(4693..6543)
                     /locus_tag="PKH58_05880"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX88590.1"
                     /translation="MKILSKINIFTGIIALMISFSSCESFLEVPSNSLISDDVVFSQP
                     DSAYKAISAIYDIIGQNNSYRNRLWIQMGINTDIEYRSGWSSGSVLSSIKSDDLFALY
                     NPNSSIGDGYNNTDAANPWSRIYQGIERANLAIEGIRKFGNPAEGNDMGHLLGTALTM
                     RAFFFYDLIKWWGDVPARFEPVNEATIYMAKTDRDTIYNQIIKDLQEASGLLYAPGTK
                     YTNTVKMVSRDAARGLLARICLSAAGYSQRPSGNDAVVKITVSDNRRRELYAIAREAC
                     RQIIADGHYKLDSDFKNIFYEQCQDVETHGREAIWELPYNMGIRGRMVYNLGLSRDAD
                     GKNNKVAISGQFKIMPNFYYDYNANDKRRDITVVPYKVVKNATLGVMEQSLSSGITGF
                     NLGKWRAEWVKTPISGTDDGVSPIVLRYADVLLMFAEADLFLGGNEGAGYFNQVRRRA
                     FSTTASTYDLPLTLENIKKERAYEFCGENIRKYDLERWGELKSSIDKAKTSLFALRDA
                     TGNYSDVPNTIYYRYVVDNSITTGERTLVIYGLQRGETDDKTVTDPTGGWTKKTWTNA
                     MTSTGELYLSDGFINNMYLGNPDKRQLLPIMHQIILASNGSLTNDYGYDN"
     gene            complement(6555..9830)
                     /locus_tag="PKH58_05885"
     CDS             complement(6555..9830)
                     /locus_tag="PKH58_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX88591.1"
                     /translation="MRKHKIRVINLALMLGLLLLNTNVFAQQRAVSGTITDQNGEPII
                     GATVIVKEGTQGTVSNVDGKFTLNVPANAKTLIFRSVGKVNKEVAITGTVLNVVLEDQ
                     QKTLDEVVVIGYGTQRKRDLTGSISSVSEKTLKDIPVATVGEALTGKLPGVQVTTTEG
                     SPDAEIKIRVRGGGSITQSNSPLYIVDGFLKDDIKDVAPSEIQSIDVLKDASSTAIYG
                     SRGANGVIIITTKTAKQGKMSLSYDGYVGFKNITKKLDVLSPYQFVQKQYERATWNNA
                     VESEYEKYFGNYEDINLYNYKNGMDWQDETFGRTGFQQNHSIALSGGSRKLKYNFSYT
                     NLTDKAIMYMSDYNRNNFSGKLNYQPIKWLKLDVSARYARTEINGSGANDVTGSEKST
                     SDSRVKNAVIYTPIELKNMVAQDDDLDAAASLYSPLLTTEDNNRYQKKNDYNINGGFT
                     LNITKNLSFRSTFGVTSSKKNDKRFYGLTSYFAREGGALKRDNQVAPSILLTNTDVKT
                     IQNTNVLNYAKDDIFKGHDLSVTLGHEMYSRRNKNTTSDIQAFPVYYNSFMAWDSISS
                     GTNRYGDAYFRDMDDRMISFFGRVNYNILDRYLLAATFRTDGSSKFSGDNKWGYFPSL
                     SAGWRISEEEFMKNLKKVISNLKLRASYGESGNNNINNSAFKRTYSVSNSAYLPSGTM
                     QTSIYTPGLLLVNPNLRWETTITRNGGLDFGFFDNRINGSFEMYSNSTENVLLQMPIT
                     GVGYTNQWQNAASTSNRGAELTLNIGLVESKNFNLNFMFNISANQNKVDDLGGLDSYS
                     FNEAWTSMTEASNSYVVKPGEPVGQIYGFVNEGWYSARDFMWNGSKWVMNTAKYINYD
                     AANKIYSDQFGNKFVDNSSIDGLSWGPGAMKLKDQNNDGKITELDRVVIGNTNPKHFG
                     SFAFNGDFKGFDFTLNFNWVYGNDIYNANKIEMSSLYYKYRNMLSSNLNSYTTIYWET
                     GKRITDIVELDLMNANNHASIWAAPTGRYAITSWAIEDGSFLRFNNMTVGYTFPAGML
                     KSLFIQKLRIYATAYNIYTFTNYSGYDPEVDTRRTTPCTPGVDYSAYPRSRSYNFGVN
                     LTF"
     gene            10212..14648
                     /locus_tag="PKH58_05890"
     CDS             10212..14648
                     /locus_tag="PKH58_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX88592.1"
                     /translation="MTTKRLLPIIAAVFIISSQLRSEPMCHIRHYSTEDGLPQYNIMD
                     MLQDKKGYLWLATWDGLSKFDGYDFRNYKAKSGNRYFMKSNRIEKLYEDAYGRIWFKS
                     YDGDTHCFNPSNEEFWGIQSIENITDDKISNIEIEPSGKIWLIMQNNGCMLLKDASFE
                     PEIFNLKNNKLPSEKVNSVLEEKNKNTWILTNNGIVAISNNNSIRRYFSEQISARKEN
                     FQAFFKGIELSSEIWFGSQNGRIWRIDKKSGKASLLQLQVQSDIVDFIRTSDHEVCIF
                     TQNAGFAVFNTTTNSIKIYNTGNLKGLTSNEIRPLHIDKKQNLWFETNHIGINRFNFK
                     TGNFKSYNVSSIDMMTFVSPPIALVIEDVKGRIWIQPKGGGFSLYNSITDTLEPFFNS
                     PTSQNWRFSSTVYSAHSDKQGNLWLSTHSHGLEKIVFDNNYFTANTISENKIPKTSNE
                     VRAVFEDDNQNLWISTKNRKVTVFDKKKQKLGELGADGSIRSINTMPAIVYCIMKDKD
                     NNIWVGTKGAGIFILNKTANPLKFNATHYSHNATDIYSLSDNSVYSIFEDKQGKMWVG
                     TYGGGLNLINKTNDGKLKFINHRNHLKNYPIEMSSQVRFVTQNRQGNICLGTTSGMIM
                     FSSVFSSPENISFKRYTVEPDSKNCITNNDVHGICITRRGDMYLFTFGGGINKVTQFD
                     KKGFPLTFKSYTRTNGLPSDVTLAMEEDEFGKLWISTENSLTKFDPEKEVFKTFSDIR
                     KLMINSNFSEVSTCKLKNNDIIFGYSDGYLSFSPRKIVGSNFKPYIALTNFQLFNKDV
                     ILDEKSPLSSIIDDIKKLKLTHKQKFFNIEYAALDYLDPENILYAYKLDGFDDDWNYV
                     QKQRIAFYTNIPRGKYIFRVKSTNSDGDWVDNERILPVEVMPSFWQTKFAWILYILVI
                     FGLLFLFFRILLSFYHLKANVELEKKMSEMKLRFFTDISHEIRTPLTMITGPVDYMLN
                     DSKTPEFMKHQLHVVSQNSNRLLRLVNQILDLRKMQFTNLKVQETDMDDLVSKICDNF
                     SEIALNQQIQFNYQNISKSPMVWIDPDAAEKIVMNLLSNAFKYTSPGKSIDVSLKDDD
                     KNLILTIKDEGVGISKEKQKKLFTRFVTFNDDKIKPSTGIGLNMVKELADKHAAKVGL
                     ESEEGVGSTFTVSFQKGFSHFGKDVEIIAASIEPSGKELQQEPHAVVCETEIHAPGHY
                     EKQSILIVEDDADLRSFVRTILEADYQVLEAEDGEAGLKTAMTSLPDFIVSDIMMPKM
                     DGVELLQALREEKTTCHIPVVLLTAKTTIETKLEGLQYGADDYITKPFSVSYFKARIA
                     NLLQQRKNLQRIYRENLFHTVDVHTESNNSVTPHEYQPKAVMIASHDDEMMLKIMDLI
                     ENNIDNTEFSVEEMGQYVGMSRSVFFKKLKSLTGLSPLEFVRDIKMKRAAQLLETGEL
                     MVKEVASRVGITDTRYFAKCFKAKYGMIPQEYKTNVGKSRRE"
     gene            complement(14709..15488)
                     /locus_tag="PKH58_05895"
     CDS             complement(14709..15488)
                     /locus_tag="PKH58_05895"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HNX88593.1"
                     /translation="MVNFSLEGKIALVTGASYGIGFALASAFAEAGATIVFNDINQEL
                     VEKGMAAYQEAGIKAHGYVCDVTNEDQVNALAAQVEKEVGVVDILVNNAGIIKRIPAI
                     EMTAAEFRQVIDIDLNGPFIVSKAFAPGMIKKGHGKIINICSMMSELGRETVSAYAAA
                     KGGLKMLTKNFASEWGEFNIQCNGLGPGYIETPQTAPIRVPGHPFNEFIIAKTPAARW
                     GKTEDLQGPAVFLASDASNFVNGHILYVDGGILAYIGKQPK"
     gene            complement(15503..>16314)
                     /gene="kduI"
                     /locus_tag="PKH58_05900"
     CDS             complement(15503..>16314)
                     /gene="kduI"
                     /locus_tag="PKH58_05900"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HNX88594.1"
                     /translation="HPEDAKQYTTARLRKDFLIEKVFSSDEVNMVYSMYDRMVVGGAM
                     PVSESLKLEAIDPLKQPVFLMSRELGIYNVGGSGLVKVGDEMFELNYKEALYLGSGNR
                     EVYFESLDASKPAKFYFNSATAHRNYPDKKVTKADAVTMELGSLETANHRVINKMLVN
                     QVLPTCQLQMGMTELAPGSVWNTMPAHVHSRRMEAYFYFEVPEDQSVCHFMGEPDETR
                     HIWMQNDQAVLSPEWSIHSAAATYNYTFIWGMAGENLDYGDQDFSKITELK"
BASE COUNT         4803 a         3452 c         3159 g         4900 t
ORIGIN      
        1 ccggttaaat cacttttctt tactatacca taaccaatca ccaccagttc gtccaacgtt
       61 tttgtgtctt cagacattgt tagattgatt aaggatttac cctcaacaga aatagtttga
      121 gatacaaatc cgacataagt aaaagtcaat tttccgttac cggaaacatt cagtgagtaa
      181 tttccgttaa tgtcagtggc tgtaccgttg gctgttccct gttcgactac acttacccct
      241 ataacaggtt caccggaaac atctttcacc tgaccgctga ctttaacttt ctgctggtcg
      301 gggatttgtt gtgcattttc cgttcctaat tcagtttttg ctgaataagg aaacgtcacg
      361 cttttgttgc ttttcgcatt cgcgaaaccg acaaacagca gagcgctgat catcagcaac
      421 aatttaactt ttgtgttttt cattcatttt gtttttaatt gtttgacatg gatatttgat
      481 tattagattt tttttcggta ttcttcagtg tgtgtgtgtt tatttaatca gttttgtcaa
      541 tggttttatt tttttttctt tcacctggct gacaaacatt tttgttacct ggcgggcacc
      601 tgcttcattg aaatgggtat tatcatcaag acctttggga taaacccggc taactccagg
      661 tggtatttta tgaaaatatt tccttgaatt ctccactcct tccttttgca accattccga
      721 agtcagcttt tccatatcaa tcagtggtac tttctgttcg gccgctacct gccggatgta
      781 tgacggatat tcaccgtgtg tgggaagcaa attccctttt tcatcaaaac ttctgcgtgc
      841 tactgctgtg catagaatcg gatttccccc ttttgctctc acatcattta cgaatgcaat
      901 gaaatttaat ctgaactgct cgggtttggt ataacttttt ttcgtttcaa cttcgtcgtt
      961 atgtccgaac tggataatta caatatctcc tttccgtaca tttttgataa gttctgtcca
     1021 caatccttct tctataaaag ttcgggtgct tcttccgttt ttagcgtaat tcaccacaac
     1081 agctttatta ttaacaaatt ccggaaacaa ctgcccccag cctctttcaa gcaatggttc
     1141 atcaaaagtt tcaccggtca gtgtatctgt caggtgtttt gttagtggtt tatcagccat
     1201 tgtagagtca cctgccatca atattttgac ttttggcggt gccatgctta caaatacaaa
     1261 agctaaaatt atcagtaaaa agtttttaac tttcatttat tttatcattt ttcctgaaat
     1321 aacgatatta ttgttaccat ttccctgaat atcaacaggc ttttcaagac cagctggata
     1381 ggtgaaatta tctgaattaa aattttccac ttctttcagt attaaagcag aaccggtttg
     1441 agggacgata tgtacattct taaaaataat gtctttcgaa ttactcagtt cggctccgag
     1501 ttgagcagta attttcacat cttcaatatg aatattactg attttcattt ctggcaggcc
     1561 gttgaaaaac atggctctgc gtgcattgcg tgaaatgaga ttttttacaa aaatatttct
     1621 gaaaacaggc gtttcttcgt taaccggata gttaatttcg ttatccgaaa ctttatcacc
     1681 gtcttcgaga gtttcagttg ccgatttacc tccgtaatac aggtcaaaaa ggaatgattc
     1741 agtttcgata ttaaacatat atatgtctga tatatagata ttttcaacaa cacctccccg
     1801 accacgggta cttttaaacc gtaaacccac atctgttcca agaaactggc agtttttcac
     1861 agagatgttg cgcaccccgc ccgacatttc acttccaacc acaaatccac catgcccctg
     1921 aaacacttta cagttatcaa caataacatt ctctgtaggt cgtccgcgca cacggcccgg
     1981 cttatcttta ccggatttaa tacaaatgcc atcatcacct acatcaaact ggcagttgac
     2041 aatgattgaa ttgcggcagg actccaggtc aagtccatct ccattctgcg aataacccgg
     2101 gtttcgaacg aacaaaccat caagtataac attttcacac attaaagggt gcagattcca
     2161 gctgggggaa ttttcgaaaa ggacaccctg aaaatataca tttttacatt cgatgaagct
     2221 aaccatcacc ggacgcagaa aatccttgat actaagccat tcttgttctg tcagattctg
     2281 acggggtaca ttcatatcac ttatttcggc accctgaaca gaactttcgg aagggtacca
     2341 cgtgtcattt ttaacaacgc cacctgatga agttactttt ttccactggc tcgcggtgac
     2401 tttgttcttt ttcaggggtc tccaggcctc gccaaaaccg ttaatactac cttttccggt
     2461 aatagcaata ttcaccaggt tacgacctga aattggtgac tggcatcggc gtgtatccaa
     2521 tccttcgaaa atcgtttcca caataggata aaggttaaaa tcgggcgaaa aaaggattac
     2581 ggctcctttt tcaaggtaca tgttgatatt cgaccggaag acaatgggtc ctgtaaacca
     2641 gatacccttg ggtacaacca gggtgccacc acccttctcc gacaatgtct ccatcgcttt
     2701 ggcaaatgca ttggtattga gcaccatgcc atcgccaact ccgccacaat ctgcaactga
     2761 aattctatta tttttgaaaa ccggcgcttt gagttttggc atcgtgaaag gcagatcttt
     2821 gtatagatac tcataggtat tttttgcctg aaggctcatc cccaaagtca tcaacgccaa
     2881 acctactgcc agaattctga ttttgttcga tattttcatt ttttagtagt gtataataaa
     2941 tgataaaaaa acaaacacaa taaattttca taattggata ttgacattta tctgcattgc
     3001 cgataaatgt caatatcccg gtttagtacc agcgcaaatc acccgaaatt cctttaccgg
     3061 caaatgaagt tgttttgagc gtaaaatcgc cgttaacagg attattccac aaatcggtcg
     3121 atgcaccatt aaatgaagtc aatttatctt tgatagtgta agttgctccg cctactaatg
     3181 tttcatcgag ataatcagtc gtaaagtatg tacctgaaat tgttttggtt cctgtggtac
     3241 gcactccggc tgctattgtt ccgttacttc cgaaaataca gtttttgatt gtcagaccac
     3301 cggttacagt catattatta agatcgacga gcaacctggt agaaccagca tcctgagtag
     3361 cctgattaaa tgtacaatta gtcaggctta cagtgcccat ggtgtatgca cccgtacgca
     3421 aaatcagcga tcctttaaaa ttgtaaacgg tacaatattc gaatgtgata ttatcgaaat
     3481 aatctttgga attactgttg acaatagcgt aagtacttga aaagcccaca tcattaatca
     3541 cacatccttt gaacttcaga tcagaaatcc gctgttcggt atttccagac atacgcatcg
     3601 gtgtattgcc aatgttgtgg atattgcaat taataaattt cagacttgca atggcgtacg
     3661 ctgttttatt attgaacaga taaccgattt ttgtggctgc cggattattg tcgcaataac
     3721 ctgtgaaatc aatattctca aaaaccagac tatcaatggc tgccgatgct tcgacaccaa
     3781 acatattggc tgtggaagtg ggcgtaccgc ttgtcataca tattacagcc cgattgaccg
     3841 gcgacgcgct tctgattttc aggtttttat tgaaattata agctgagccg ccaatcggga
     3901 atgtagcgcc tcctgcaagt aaaattacct gtccggatgt agcgttggtc attgccgaaa
     3961 gcaggtcttc accggctgaa agataaatat caccctcggt taaaccataa gttgtaccac
     4021 gtagtatctt tccattgtat attcgggtct tccacaaggc gttggcaggt aaactcacaa
     4081 ttttcgatcc tgctgcagct tcggaagcgg agataagcac agagtctttt aatccgccat
     4141 cggcagtcag tatcagatgt gtggtatttg caccgggtaa ccattttaca gtaagcgttt
     4201 ttgtggcagt catataggca gaataaagca caccagtatt atttcccgtt ccgtaaccct
     4261 gaaagagatt ttcttttgga gttttgaatg aaagagtagt attgaatttt gatgattttg
     4321 attcaatgcc agagttagca cggatacgtg cataaaactt cgtatcaccg gccagttctt
     4381 taacaacaga tagctttgtg gttgtaatat cgagtatcgg actggaataa tccagactat
     4441 cgttgctgag ttggagtgta taggatactg cactgtccac aggtgaccag gttatggtgg
     4501 caatggtttt attcagctct acttgaaaat tgattggccg aaacagatag ggcaattgta
     4561 attcgggctc atcctccgtg caagccgaaa atgtacccga aaccatcaga agaattacaa
     4621 caattgcggc aaatatattt ttatttcttt tcattgttct gattacttaa tgtgttatta
     4681 attgtcaatc tgttagttgt cataaccata atcgttagtc agcgagccat tgctggcgag
     4741 tataatctga tgcatgattg gaagaagctg acgtttatcc gggttaccca gatacatgtt
     4801 attgataaat ccatcgctca gatatagttc acccgttgaa gtcattgcat ttgtccatgt
     4861 ttttttggtc cagccaccgg taggatcggt tacagtttta tcgtccgttt cgccacgctg
     4921 cagaccataa attacgagtg ttctttcgcc ggttgtaatg ctgttatcca ccacatacct
     4981 ataataaatc gtattcggca cgtcggaata gtttccggta gcatcgcgga gtgcaaacaa
     5041 actggtttta gctttatcga tggatgattt aagttcaccc cagcgttcca ggtcgtactt
     5101 gcggatattt tcaccacaaa attcataagc tctctctttc ttgatatttt cgagagttaa
     5161 cggcaaatcg taagtcgatg cagtagtgct gaaagcacga cgacgtacct gattaaaata
     5221 accggcacct tcatttcccc ccaggaaaag atctgcttct gcaaacatta ggagtacatc
     5281 tgcgtatcga agcacaattg gcgacacgcc atcatctgtt cctgaaattg gtgttttgac
     5341 ccattcggca cgccattttc ctaaattaaa accggtaatg ccactggata gcgattgttc
     5401 cattactcca agagtcgcat ttttaacaac cttgtaagga actacagtta tgtcgcggcg
     5461 cttatcattc gcattataat catagtagaa attgggcata atcttgaact gaccactgat
     5521 ggcaacttta ttgttttttc catcagcatc gcgcgataaa cccaggttgt aaaccattcg
     5581 gccacgaata cccatgttgt atggtaattc ccagattgct tcacgtccgt gagtttccac
     5641 atcctggcac tgttcgtaga atatattttt aaaatcacta tccagcttat aatgtccgtc
     5701 tgcaataatc tgacgacaag cttcgcgggc aatggcataa agctctctgc gacggttgtc
     5761 ggacaccgtg attttcacca ctgcatcatt gcccgaagga cgctgcgaat aaccagccgc
     5821 cgaaaggcag atgcgggcca ataatccgcg ggctgcatcg cgactaacca tttttactgt
     5881 attcgtatat tttgtaccgg gtgcatacaa tagtccggat gcttcctgca aatctttaat
     5941 aatctgatta taaattgtat cgcgatcggt tttagccata taaatggttg cttcatttac
     6001 cggttcgaag cgtgcaggta catctcccca ccatttaatc agatcataga agaaaaatgc
     6061 tctcatggtt agtgctgttc cgagcaaatg tcccatatca tttccttctg ctggatttcc
     6121 aaattttcga attccttcta tagccagatt agcacgttca attccctgat aaatacggct
     6181 ccagggattg gctgcgtctg tattgttgta accatcgcct attgacgaat taggattata
     6241 gagggcaaac aggtcatcgg attttattga agacagcaca gaacctgatg accatcctga
     6301 tcggtattcg atatccgtgt ttattcccat ctgtatccac aacctgttac ggtacgagtt
     6361 attttgtccg atgatgtcgt aaatggcgct gatagctttg taggccgaat cgggctgact
     6421 gaaaaccaca tcatcactaa tcaatgaatt gcttggcacc tccagaaaac tttcgcacga
     6481 tgagaatgat atcatcagag caatgatgcc tgtaaatata tttattttgc taaggatttt
     6541 catagtgata tgatttaaaa ggttaagttg acaccaaaat tatatgaacg gcttcttgga
     6601 tatgccgaat aatctacgcc cggagtacag ggtgtggtac gacgcgtatc cacttctgga
     6661 tcgtaaccag agtagtttgt aaaggtgtag atgttgtacg cagtagcata aatacgcaat
     6721 ttctgaatga acaggctttt cagcattcca gccggaaaag tgtaacccac tgtcatattg
     6781 ttaaaacgca ggaatgaacc atcttctata gcccacgaag tgatggcata acggcctgtt
     6841 ggcgcagccc aaatagaagc atgattattg gcattcatca gatccagttc tacaatatcc
     6901 gtaattcgct tgccagtttc ccaataaata gttgtgtacg aattgaggtt ggatgacagc
     6961 atattgcggt acttgtaata cagcgatgac atttctatct tgttggcatt ataaatatcg
     7021 ttgccataca cccagttgaa attcagtgta aaatcaaatc ctttgaaatc accattgaag
     7081 gcaaatgaac caaagtgttt cggattggta ttaccaatca caacacggtc aagttcagta
     7141 attttaccat cgttgttctg atccttcagt ttcatggcac cgggacccca gcttagtccg
     7201 tcaattgaac tattatccac gaacttattt ccgaactgat ctgaataaat tttattagct
     7261 gcatcatagt taatgtattt tgcggtattc atgacccact tggaaccatt ccacataaaa
     7321 tcgcgggcac tgtaccaacc ttcgttaaca aaaccgtaaa tctgaccaac cggttcccca
     7381 ggtttaacaa cataactatt cgatgcttcg gtcatactag tccaggcttc gttaaacgaa
     7441 taggaatcga gacccccaag atcatcaacc ttattctgat tggcgcttat attgaacata
     7501 aagtttagat taaaattttt gctttccact agcccgatat tgagtgtaag ttcagctcct
     7561 ctgttcgatg tggatgctgc attttgccac tgattggtat aaccaacacc cgtaatcggc
     7621 atctgaagca atacattttc ggtagaattg gaatacattt caaacgatcc gttgatgcgg
     7681 ttgtcgaaaa atccgaaatc tagtccacca ttgcgagtga tagtcgtttc ccatcgcaga
     7741 tttggattga caagcaataa accgggagta tagatgcttg tttgcatggt tcccgaaggc
     7801 agatatgccg aattcgacac agagtaagtt cttttgaatg ctgaattgtt gatattgttg
     7861 ttacccgatt cgccgtaact tgcacgcagt ttcaagtttg aaatcacctt tttcaggttt
     7921 ttcataaatt cttcttccga tatacgccat ccggctgaaa gtgaagggaa atatccccat
     7981 ttattatcac cggaaaattt gctcgaacca tcggtacgga atgttgcagc tagcagataa
     8041 cgatccagaa tattgtaatt tacacgtccg aagaatgaaa tcatacggtc atccatatcc
     8101 ctgaaatagg catctccata acggtttgta ccagagctga tactatccca ggccataaat
     8161 gaattgtaat acaccgggaa agcctgaata tcactggtcg tgtttttatt gcgtctgctg
     8221 tacatttcat ggcctaaggt aaccgataaa tcatgtccct tgaaaatatc atccttagcg
     8281 tagttgagta cattggtatt ttgaatggtt ttaacatcgg tatttgtcaa caaaatagag
     8341 ggtgccacct gattatcacg tttcagtgca ccaccttcgc gggcaaaata ggatgtaagt
     8401 ccataaaatc gtttatcatt ctttttactg ctggtaacac cgaaggtact cctgaacgaa
     8461 agatttttag taatatttaa tgtaaatcct ccgttgatgt tataatcatt ttttttctga
     8521 tatctgttgt tatcctcagt tgttaacagt ggtgaataca aacttgctgc cgcatccaga
     8581 tcatcatcct gagccaccat atttttaagt tcgatagggg tataaattac ggcatttttt
     8641 acacgcgaat cggaggttga cttttcggat ccggtcacgt cattagctcc cgaaccatta
     8701 atctcagttc tggcataccg ggcagagaca tcgagtttta gccatttaat aggctggtaa
     8761 ttgagcttac cgctgaagtt gtttcggtta taatccgaca tgtacatgat tgctttatcg
     8821 gtcaggtttg tatagctgaa attgtatttc aactttcttg aacctccgga aagagctatc
     8881 gaatgatttt gctggaaacc ggtgcggcca aaggtttcgt cctgccagtc cattccgttt
     8941 ttataattgt acaggttgat gtcttcgtag ttgccgaagt atttttcata ttcggattca
     9001 acggcattat tccaggttgc cctttcatat tgtttctgaa caaactgata aggacttaac
     9061 acatcaagtt tttttgtgat attcttaaaa cctacataac catcgtacga gagtgacatc
     9121 ttaccctgtt tggctgtttt ggttgtaata ataattactc cgttagcacc ccgcgacccg
     9181 taaatagcag tcgaagatgc atctttgagc acgtcgatgg actgaatttc tgagggcgct
     9241 acgtctttga tatcatcctt gagaaatcca tcgacaatat acagtggcga attgctctga
     9301 gtgatagaac cgcctccgcg tacacggatt ttaatttctg catccggcga gccttcggtg
     9361 gtggttacct gtacgccggg aagttttccg gtaagcgctt ctccaactgt agctacagga
     9421 atatctttca gggttttttc gctgactgaa gatattgatc ctgtcagatc tctttttcgt
     9481 tgggtaccat aaccgattac aactacttcg tcaagtgttt tctgttgatc ttcgagcaca
     9541 acattcagta cagttccggt gatagcaact tctttattta cttttcctac agaccggaat
     9601 atcagcgttt ttgcattggc aggaacgttt aatgtaaatt ttccatccac attggacact
     9661 gttccctgag ttccctcttt tacgattacc gtagcaccaa ttattggctc accgttttgg
     9721 tctgtaattg ttcctgaaac agctctttgc tgggcaaata catttgtatt caggagcaac
     9781 agtccaagca ttagtgccag gttaatcact ctgattttgt gttttcgcat attgattgaa
     9841 taattaaagg ttgaattctt ttatgtaaat gatgttttat cgatgtaaaa gtatttttga
     9901 tgattatcag gcaacacaat aaaacattag ttaatttttt attattttaa ttacaatatc
     9961 cgatgcaatt atagtaatta taatacaaac caaggtgtgt catttcaatt tttgaagtgt
    10021 gacaaattgg ctcagataaa aatacaatca tgctgcaaca gtaattccat agaaaaaaag
    10081 gacattaggt attctgcaaa ttggaaaatt tacttaaata tcgtaatttt gtagaaatgg
    10141 tacgctaaca ttccggatta agttgtcagc tacgtattaa aacaccataa ttcacgtcat
    10201 ttaaacaata tatgactaca aaaagattat tgcctataat tgctgctgtt tttattatca
    10261 gctcccagct ccggtctgag cccatgtgtc atatcaggca ttattcgacc gaagacggac
    10321 tgcctcagta taatattatg gatatgctgc aggataagaa aggttatctg tggctggcaa
    10381 cctgggacgg attaagtaaa tttgacggct atgacttccg gaattacaaa gcaaaatccg
    10441 ggaaccgcta cttcatgaaa agcaatcgta tagaaaaact ctacgaagat gcttatggcc
    10501 ggatttggtt taaatcgtat gacggcgaca cgcattgttt caatccttca aacgaagagt
    10561 tctggggaat tcaatctatc gaaaatatca cggatgataa aatctcaaat atcgaaattg
    10621 aaccttcggg taaaatatgg ctgataatgc aaaacaatgg ctgtatgcta cttaaagatg
    10681 cttcgttcga accggaaatc ttcaatctga aaaacaataa acttccctct gaaaaggtta
    10741 acagcgtact cgaagaaaaa aacaagaaca cctggatact taccaacaac ggaattgttg
    10801 ccatatcgaa taataattca attagaagat atttcagtga acaaatttcg gcacgcaaag
    10861 aaaatttcca ggcttttttc aaaggtatcg aactcagcag tgaaatatgg ttcggatcac
    10921 aaaacgggcg catctggcgg attgataaaa agagcggcaa agcaagtctg ctacaacttc
    10981 aagtgcaatc ggacattgtt gattttataa ggacgtcaga ccatgaagtg tgtatcttca
    11041 cccagaatgc aggatttgct gtttttaaca cgacgacaaa tagcataaaa atatacaata
    11101 caggcaatct gaagggactg acttcgaatg aaataagacc tctgcacatt gacaaaaagc
    11161 aaaacctgtg gttcgagact aaccacatcg ggattaaccg gtttaatttt aaaaccggca
    11221 attttaaatc ttataacgtg tcatccattg atatgatgac ttttgtttcg cctccgatag
    11281 cactcgtcat cgaagatgtc aaaggaagaa tatggataca acccaaagga ggcggttttt
    11341 cgctgtacaa tagcatcacc gacacactgg aacctttctt caactctccc acctcacaaa
    11401 actggcggtt ttcaagtact gtttattctg cacattccga caagcagggt aatctgtggc
    11461 ttagcacaca ctctcatggg ctcgaaaaaa ttgtatttga caacaattat tttacagcta
    11521 acaccatatc tgagaataaa attccgaaaa cttccaatga agtcagagct gtattcgaag
    11581 atgataatca aaatctctgg atttcgacta aaaacaggaa agtaactgta ttcgacaaaa
    11641 aaaagcaaaa attgggtgaa ttgggagccg acggaagcat cagatccatc aatacaatgc
    11701 cggcaattgt ttactgcata atgaaggata aagacaataa tatttgggtg ggtacaaagg
    11761 gtgccggtat ttttatcctg aataaaactg ctaatccgct caaattcaac gcgacacatt
    11821 acagtcataa tgccacggat atttacagcc taagcgataa cagtgtttac agcatatttg
    11881 aagacaaaca gggaaaaatg tgggttggaa cctacggagg cggacttaat ctgataaaca
    11941 aaacgaatga tggaaaactg aaatttatca atcaccgtaa tcatctgaag aattacccca
    12001 tcgaaatgtc atcacaagtg cgattcgtca cacaaaacag acaaggaaat atatgcctgg
    12061 gtaccaccag tggcatgatt atgttttcat ccgttttttc atcacccgaa aacataagct
    12121 tcaaacgata caccgtagaa cccgacagca aaaactgcat aaccaacaac gatgtacatg
    12181 ggatttgcat aacacgcagg ggtgatatgt atcttttcac attcggcggg ggaattaata
    12241 aagtaacgca attcgacaaa aagggttttc cgcttacttt taaatcctat accagaacca
    12301 acggactgcc ttcggacgtt acactggcta tggaagaaga tgaatttggc aaactctgga
    12361 tcagtaccga aaacagtctt acaaaatttg atcctgaaaa agaagttttc aaaacttttt
    12421 cggacatacg caaactgatg attaacagca atttttcaga agtcagcacc tgcaaactga
    12481 aaaataatga tatcattttt ggttattccg acggatattt gtcattttca ccccgaaaga
    12541 tagttggcag caacttcaaa ccgtacattg ctctcactaa ttttcaactt ttcaataaag
    12601 atgtaattct tgacgagaaa tcacctctat cgtcaatcat tgacgacata aaaaaactca
    12661 agctgactca caaacaaaaa tttttcaaca tcgaatacgc tgcgctggat tacctcgatc
    12721 ctgaaaatat actttacgct tacaagctcg atggtttcga cgatgactgg aactacgttc
    12781 agaagcaacg gattgccttc tacacaaata taccaagggg taaatatatt tttcgcgtga
    12841 aatctaccaa cagcgatggc gattgggttg ataatgagcg aattctgcct gtcgaagtga
    12901 tgccatcgtt ctggcaaact aaatttgcat ggattttata tattctcgtt atcttcggat
    12961 tattgtttct gttcttcaga attttactct ccttctatca tctgaaagcg aatgtggaac
    13021 tggagaaaaa aatgtcggaa atgaaactac gcttcttcac cgatatttct cacgaaatcc
    13081 gtacaccttt aaccatgatt accggtccgg tggattatat gctcaacgac agcaaaacac
    13141 ccgaatttat gaaacatcag cttcatgtcg tttcacagaa ttcgaaccgt ctgctgagat
    13201 tagtcaacca gattttggat ttaagaaaaa tgcagttcac caacctgaaa gtacaagaaa
    13261 cggatatgga cgatttagtt tcaaaaatct gcgacaattt ttcagaaata gctctaaatc
    13321 agcaaattca attcaactac cagaatattt ctaaaagtcc gatggtatgg attgatcctg
    13381 acgcagccga aaaaattgta atgaatttgt tatccaatgc cttcaaatac acttctccgg
    13441 gtaaatcaat cgatgttagt ctgaaagacg atgataaaaa tctgattttg acaataaagg
    13501 acgaaggtgt gggtatttcg aaagaaaaac aaaaaaaact ttttacccgg tttgtaacct
    13561 tcaacgacga caaaataaaa ccaagtaccg gtatcggact gaatatggtg aaggaacttg
    13621 cagataaaca tgctgcgaaa gtcggtcttg aaagtgaaga aggcgttgga agcaccttta
    13681 ctgtaagttt ccagaaagga ttttcacatt ttggcaaaga tgtggaaata atagctgcca
    13741 gcatcgaacc atctggcaaa gaattacagc aggagcctca tgcagttgtt tgcgaaacag
    13801 aaattcatgc acccgggcat tacgaaaaac agtcaattct cattgtcgaa gacgatgccg
    13861 atcttcgttc ctttgtccgg accattctcg aagccgacta tcaggtgctc gaagccgaag
    13921 acggagaagc aggactgaaa acagccatga ccagtttgcc ggactttatt gtaagtgata
    13981 tcatgatgcc caaaatggac ggtgtggaac tgttacaggc tttacgcgag gaaaaaacga
    14041 cctgtcatat accggttgtg cttctgacgg caaaaactac catagaaacc aaactggaag
    14101 ggttacaata cggtgcggac gactacatta ccaagccgtt cagcgtttcg tattttaaag
    14161 cacgaattgc aaacttattg cagcaacgga aaaatttaca acgcatttat cgcgagaatc
    14221 tttttcatac tgtggatgtt catacggaaa gcaataattc ggtcacacct cacgagtatc
    14281 agcctaaagc agttatgatt gcttcgcacg atgacgaaat gatgctgaaa attatggatt
    14341 tgattgaaaa caatatcgac aataccgaat tctccgttga agaaatggga cagtatgttg
    14401 gcatgagccg ctcggtattt ttcaaaaaac tgaaaagtct gaccggactg tcgccactgg
    14461 aatttgtgcg ggatattaag atgaaacgtg ccgcacagct actcgagacc ggtgaactga
    14521 tggtaaaaga agttgcctcc cgggtcggga taacagacac acgctatttt gccaaatgtt
    14581 ttaaggcaaa atacggtatg atacctcagg agtacaagac aaacgtcggg aaaagcagac
    14641 gggaataact aaaaaaaggt cgggagaaaa ctcctgacct ttttaaatga ttatatatct
    14701 gatatgcgtt attttggctg tttaccgata taagccagaa ttccgccatc aacgtacaaa
    14761 atatgaccgt taacaaagtt ggatgcatct gaagccagga aaacagcagg tccctgcagg
    14821 tcttcagttt ttccccatct ggctgccggt gttttggcaa taataaattc attgaacgga
    14881 tgaccgggca cacggatggg tgcagtctga ggagtttcga tatatcccgg accaaggccg
    14941 ttacattgaa tattaaattc tccccattcc gatgcaaaat ttttggtcag catcttcaaa
    15001 ccgccttttg ctgcagcgta tgcgcttacc gtttcacgtc cgagttcact catcatcgaa
    15061 cagatattga taattttacc atgacctttt tttatcatgc cgggagcaaa tgcttttgac
    15121 acaataaaag gtccattcaa atcaatgtct atcacctgac gaaattcagc tgcagtcatt
    15181 tcaattgccg gaattcgttt gataatacct gcattattga ccagaatatc aacaacccca
    15241 acttcttttt cgacctgagc agctaatgcg ttaacctgat cttcatttgt gacatcacaa
    15301 acataaccat gggctttaat accagcttcc tgataagcag ccattccttt ttcgaccagt
    15361 tcctgattga tatcgttgaa tacaatagtt gcgccggctt cagcaaatgc agaagccaga
    15421 gcgaaaccga ttccgtaaga tgcgccagta accagagcaa tctttccttc taatgaaaaa
    15481 tttaccatta tattttcttt ttttatttaa gttctgtaat tttagaaaaa tcctgatctc
    15541 cgtaatccag attttcaccg gccatacccc agataaaagt gtaattgtat gtagccgcag
    15601 ctgagtgaat agaccattcg ggagaaagca cagcctgatc attctgcatc caaatatgac
    15661 gcgtctcgtc gggttcgccc ataaaatgac acacagactg atcttcgggc acctcaaaat
    15721 agaaatatgc ttccattcgg cgtgagtgaa catgagcagg catggtattc catacactgc
    15781 caggagcaag ttcggtcatt cccatttgca gttgacaggt gggtaacacc tgattgacga
    15841 gcatcttatt aatcacccgg tgattagccg tttcaagtga accaagttcc atggtaacag
    15901 cgtcggcttt ggtaaccttt ttatctgggt aatttcggtg agcagtagct gaattgaaat
    15961 aaaattttgc aggtttcgag gcatccagac tttcaaaata tacttcccgg tttcccgagc
    16021 ccagataaag cgcttctttg taattcagtt caaacatttc atcaccaact ttaaccaaac
    16081 cacttcctcc gacattataa attccaagtt ccctgctcat taagaacacg ggttgtttca
    16141 gcggatcgat tgcttcgagt ttcaggcttt cactgacggg cattgcacca cctacaacca
    16201 tacggtcgta catggaataa accatattca cttcgtctga tgaaaatact ttttcaatca
    16261 aaaaatcttt acgaagtctt gctgtagtat attgctttgc atcctcggga tgcg
//
LOCUS       DAOPYB010000054        15849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3962_length_15849_cov_10.928391, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000054 DAOPYB010000000
VERSION     DAOPYB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15849
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3962_length_15849_cov_10.928391"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1978)
                     /gene="topA"
                     /locus_tag="PKH58_05905"
     CDS             complement(<1..1978)
                     /gene="topA"
                     /locus_tag="PKH58_05905"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNX88595.1"
                     /translation="MSNNLVIVESPAKAKTIEKFLGKDFEVMSSMGHIRDLGEKGLGI
                     DFENNYAPQYEVSADKKKIVAELKKEAKKATTVWLASDEDREGEAIAWHLADELKLKK
                     ENTRRIVFHEITKDAILHAIEHPREIDLNLVDAQQARRVLDRIVGFELSPVLWRKVRP
                     SLSAGRVQSVAVRLIVEREREINLFTPEAAYRVTAVFSSTDANGTPIELKAELSQRFD
                     KKADAMAFLELCKNAQFNVDSIEKKPAKKSPAPPFTTSTLQQEAARKLGFSVSQTMMV
                     AQRLYESGKITYMRTDSVNLSALALGTAKSEIISMAGEKYVHTRNFTTKTKGAQEAHE
                     AIRPTYISAHSIEGTAQEKRLYDLIWKRTIASQMADAELEKTNIYINVSGGNYQFVAS
                     GEVIVFDGFLKVYLESTDDESEAENESILPVMKKGENLTATEIMAQERFTQRPPRYTE
                     ASLVRKLEELGIGRPSTYAPTISTIQNRKYVEKGDRPAQKRTYNELKLKNGKITDQAK
                     SENTGAEKSKLFPTDIGLVVNDFLIEYFPDILNYNFTANVEKEFDHIAEGKVKWTKSI
                     DTFYQKFHPVVESTLKNSERQVGERILGTDPASGRQISVKIGKYGPIAQIGTSEEEEK
                     PVFASLRRNQSIESVTLEDALDLFKLPRELGEFEE"
     gene            complement(2125..2334)
                     /locus_tag="PKH58_05910"
     CDS             complement(2125..2334)
                     /locus_tag="PKH58_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNX88596.1"
                     /translation="MEKTAIIKGMSCSHCAARVEKALNKIDGVEAKVDLENRSAKLIL
                     TKEVSDKKIKEAVEAIDYEVTDILD"
     gene            complement(2361..4643)
                     /locus_tag="PKH58_05915"
     CDS             complement(2361..4643)
                     /locus_tag="PKH58_05915"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNX88597.1"
                     /translation="METKKFDVVGMTCSACVNHVEKSVRKLYGVQDVSVNLLTNSMTV
                     DYDQQALSVEEIIGSVEDAGYEAFEKEKSIVSTTTPKVNYALEEQKEMKRRVIISFLF
                     LVPLLYLSMGHMLDFPLPSVFTGSDNALTFAFTQFLLTLPIAFVNSKFFSHGFRSLWK
                     LSPTMDALVAIGSSAAIIYGVFAIYKIGNGLGQNNPETVRMYLHNLFFESASTILTLV
                     TLGKYLEAKSKRQTSDALTKLIDSAPQTATVIRNNTEIEIPVEEVRLNDIVSVRPGQK
                     IPVDGVLTGGSGSVDESALTGESIPVYKQEGDHVLSASINGSGYFTFRATKVGKDTTL
                     SQIIQLMEEASSSKAPISKLADQISRIFVPAVIGIALLATAVWLNMGYPFEFALSIGI
                     SVLIISCPCALGLATPVAIMVGTGKGAECGVLFKSAEALETGGKITTVLLDKTGTITE
                     GRPEVTDIEPIHYLDKNELLRITASLEKFSEHPVSGAIIQKAAEKEIELYPVSDFNNI
                     PGKGISGKIKDKIVRVGNLSFISENTNTVDIENQIHHYSQKGKTPLIVADEKNVLGII
                     AVADVLKQTSKEAIELLKSMNLKTMMVTGDNKRTALAIQEKLGLDEVIAEVLPQDKDK
                     VVQQLQATGEKTAMVGDGINDSPALIRSDLGFAIGNGTDIAIEAADVVLMKNDLLGVV
                     TGIRLSKAVIRNIKQNLFWAFFYNIIGIPLAAGVFYAGLGWKLSPMFAAAAMSLSSVT
                     VVSNALRLKKFKPTKSQTIR"
     gene            complement(4750..5703)
                     /locus_tag="PKH58_05920"
     CDS             complement(4750..5703)
                     /locus_tag="PKH58_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNX88598.1"
                     /translation="MNKNVVIWILLTCWIISGIRAYSQTDISMATHWYNRGNYNPASI
                     ARTGYIYLFSNIRNQWTGITGAPKVENVQVSGYLNSINSAFGLSVVNDNIGLTNAFNP
                     MINYAYRVSDNQTWSLSFGVSVGAYIRTIDFASYAPVTDLDPALLRDIEPQTKPDANI
                     GVEFQNDNFIFGISSTHLFSIGKNSNLFLNDNHRYGFAIYKNTRSEIMNYYLGLQVVN
                     RSNIFVLEGNGIVRFKHPTGLSTGSREIFDIGLTYRSSRQVTALLGVNLTENFRIGYA
                     YDQSFITGYNQNGTHEIMLEYRIPYENAVCKTCRDQQLWYR"
     gene            complement(5711..7165)
                     /locus_tag="PKH58_05925"
     CDS             complement(5711..7165)
                     /locus_tag="PKH58_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX88599.1"
                     /translation="MEQKIIIFLIGVLVCLSPTISTKAQSTTYGDTIIFENFGEHIHR
                     VECQYVPAGSYTFASETGDNKQRTIEDNYYTVVDPAHIKDAILTGDSYFWTAPSLSSV
                     TSTGSTRYFTEDHTEKDHNGAVMVINAGLTSNHIYKRDLKLKTGFRYLFSFWIYVVAK
                     SSQFSMEAINNTNGKKYTYSGPLLTDEGKWIRYDLNFSVPTSATLATNDVTIGLLNSY
                     KLIMGNDYYIDDILISTLHTDPLSIDVSNTSPVCEGSNMQLIVNSAGDALPFTYSWTG
                     PNGFSSEESSPVISNCTTGMSGIYNVTVTDALGQVATAATKVVINTVPKSDFSVSREK
                     IDNKNNNVTFSITPEDGVNYEWDLGDGTIENGNSFIHTYPINGNTYEYKIQLKSTLLA
                     GCSSFSTKTIEVAPYIPNVFSPNGDGVNDIFMAGYDLQVFDRAGNSLYKGNTGWDGTY
                     HGKTADNDTYFYLINYKDKNQSLKEIKGFITLKK"
     gene            complement(7179..9257)
                     /locus_tag="PKH58_05930"
     CDS             complement(7179..9257)
                     /locus_tag="PKH58_05930"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX88600.1"
                     /translation="MLFVFLTGFMNTGLADEPYCTNIGFELHNFTNWRAYTWADGQSG
                     YVTSTPKVEGIVNGRHTIITSNGYDPIVGGTQLKLIPDGYSTSVKLGSTSLGNGGLRQ
                     SLTYQLDVTPQNAFIVYNFAVVLQDPHDSSHFPEDEPRFKVSILDKDGNEITGEASCA
                     NYDVNASNANVEGWNTIQYDGKPLYWRDWTTVGINLTKYIGQTVTLEFMSANCRRKGH
                     FGYAYLTAHCQPLYITVDYCADDKIATLTAPIGFKSYQWKDSLNNPVFTGQTLQLSSN
                     VISEGDTYTCEMQSATGCDATLSATIFRYSPNADFTHSQVDCNKVNNTIIFSITSPST
                     HGTLDYHWDFGDGSTTTDQNPIHDFHSVSGMIPVTLVVKNPPSTCTDSVTKMVETFFP
                     PLIGIDGDSTYCPGETTLLKAHGAHHYEWFDGSTADNITVGKDTEVWMIGYSSNNCHT
                     DTIRVNVKKEPAWTLTTSGSELFCSGGSTVLSASGAATYSWSTGETSDSIRVDKPGSY
                     TVTGKNRRGCEKQFTFTVNEDPLPDAGFNMSVNKIDLHNNELSCSVTAQNGVNYHWDM
                     GDGKTEQGTRITHKYDVDPNLTNYKITLTAVNANGCRNSASKMVNVVLFIPNVFTPNG
                     DGINDKFMDGKEIIIYDRNGNILYKGDNGWDGNYKGEKADNDTYFYYIKYIDTDGSEK
                     TAKGFIQLKR"
     gene            complement(9827..11206)
                     /locus_tag="PKH58_05935"
     CDS             complement(9827..11206)
                     /locus_tag="PKH58_05935"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HNX88601.1"
                     /translation="MDFQNYPAEPFRIKSVEYVKMNTREEREAVIKKAGFNTFLIDSE
                     DVYIDLLTDSGTNAMSDRQWAGIMMGDEAYAGSRNFYHLYQTVQEYFGFKYMVPTHQG
                     RAAENILSQIAIKPGQYVPGNMYFTTTRFHQEKNGGIFVDIIRDEAHDASLNVPFKGD
                     IDLKKLEKLIMDKGADKIAYVCLAVTVNLAGGQPVSIKNMREVRELTRKHGIKVFYDA
                     TRCVENAYFIKEQEEEFKDKSIKEIVHEMFSYADGCTMSGKKDCLVNIGGFLCMNDEK
                     LFEDAKELVVVYEGMPSYGGLAGRDMEAMAIGLKESMQYEYIEHRIKQVRYLGDRLLE
                     AGIPIIEPVGGHAVFLDARRFCPHLTQDQFPAQSLAANLYLESGVRSMERGIVSAGRD
                     KITGENHRPKLETVRLTIPRRVYTYRHMDLVADAVIKLYQNKEQIRGLRFIYEPKQLR
                     FFTARFEEI"
     gene            11783..12397
                     /locus_tag="PKH58_05940"
     CDS             11783..12397
                     /locus_tag="PKH58_05940"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HNX88602.1"
                     /translation="MKRLGIFLLGVFLLVGSMAAQDQAKQDTAKAVTTDSLEQLYMNE
                     DLDAREAILLKKLDAKQLMDLETLRLNEQGKNNMPFSGLGLVLICLAPFIMVILIVYF
                     ASRESAKKAKQRYDISMKALELGQPLPQSFFDEPKKRTSRLQSGLVWLGVGIAIVIGS
                     FVLSKENLFLFGIIPAFVGLGILIAYLVERPKADNANNTPATNE"
     gene            12390..12932
                     /locus_tag="PKH58_05945"
     CDS             12390..12932
                     /locus_tag="PKH58_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX88603.1"
                     /translation="MNKAEDMLLVSQVVIADDHRAFDRLVQKYQSPIRRFLINLTMGD
                     TMLSDDLAQETFIKAYLNIRSFQGISAFSTWLYRIAYNTFYDSVRAKRGYDDLNIADL
                     DKKYSTEIEYSAEKNDIFAALKLLRQDEQAAILLCYMEDKTHREIAKIMNIPLGTVKT
                     NILKGKEKMGKFLIQAGYGN"
     gene            12922..13239
                     /locus_tag="PKH58_05950"
     CDS             12922..13239
                     /locus_tag="PKH58_05950"
                     /inference="COORDINATES: protein motif:HMM:NF027794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HNX88604.1"
                     /translation="METNEDKLLKSFFSQNKPEIADNGFTQKVMRRLPENEQKTGWII
                     LMFTALGCLITFALVDVPEVISEIFTFLLGVPIYYLLGGFMLIPIVTLVVMIAYRREY
                     NLA"
     gene            complement(13344..13622)
                     /locus_tag="PKH58_05955"
     CDS             complement(13344..13622)
                     /locus_tag="PKH58_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88605.1"
                     /translation="MASRRKLKKTMQFIASELITDVFFKTLLSKKDVSEKADVLAVDI
                     SKMATEFRARAGHYDSKNNPKLVKDYYRKVYSDWNAAVEKKIKDIDEL"
     gene            13825..15252
                     /locus_tag="PKH58_05960"
     CDS             13825..15252
                     /locus_tag="PKH58_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX88606.1"
                     /translation="MLIPSCIDESISADTSLKLEFSADTLQFDTVFTTIGSSTSQIKV
                     YNPNKKNLKISAIGLGMGANSPYRINVDGSTSKNNQFTDIELRAGDSLFVFVEVTIDP
                     LKTNAPVFVKDSIVFLTNSNHQDVKLLAYGQDMEILSNRTIRNDTTLTGEKPYLVYGD
                     LVVDSAKTLTLNPGCRLYFHNQASLVVYGNLIANGTRELPVLMRGDRTDRIFTDIPYN
                     YVSNQWGGVLLLNEAGKHKLNNVTMNSGYAGVYLSNQNRNKRPSLEMTNCRIHNFLKY
                     GLVVQNADVLVANTEISNTGAYSVYLNGGKHTFIHTTIANYFNSTNVRIQPSARENYA
                     ALMIMELNRIIPMETVFENCIISGSSTNEMDIMTRFSEQYHGLFSNCYIRKTKPANEN
                     PVYSHISWYDKNKRDTVFVNTYFDNEKLVYYNFMPDSVSPARNIGSMDAALKYPFDIN
                     GNSRLEDNKPDAGAIEWQPVKKTAK"
     gene            15275..>15849
                     /locus_tag="PKH58_05965"
     CDS             15275..>15849
                     /locus_tag="PKH58_05965"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNX88607.1"
                     /translation="MKKLSVILFIFVVCLSAKAQGHEKQNFRIVSYNVENYFDVVNDS
                     LTIDEEYLPEGLRAWNITKYQTKQNNISKVIAAIGGWDAPALVALCEIESEKCMRELT
                     GFSPLKNLGYKYLHHESPDARGVDVALMYQPDMFVPFHDEAIRINFPETNSTTRDILF
                     ASGLLPNNDTLHVFVCHFPSRLGGELESEDRR"
BASE COUNT         4364 a         3245 c         3281 g         4959 t
ORIGIN      
        1 tttcctcaaa ttcgcccagt tcgcgtggaa gtttgaataa atcgagtgca tcttccagtg
       61 taacgctttc aatcgactgg ttgcggcgaa gggatgcaaa aaccggtttt tcttcttctt
      121 ccgaagtccc gatttgtgct atcggaccat atttgccaat ttttaccgaa atctgccgtc
      181 cggatgccgg atcagttccc agaatccgtt cgccaacctg acgctccgaa tttttcaggg
      241 tgctttccac tacaggatgg aatttttgat agaaagtatc aatggatttg gtccatttca
      301 ctttaccttc ggcaatgtgg tcaaattctt tttcgacatt ggcagtgaaa ttataattca
      361 ggatatccgg aaaatattcg atcaggaagt cgttgacaac cagtccgata tcggtaggga
      421 atagttttga cttttctgca cctgtatttt ctgattttgc ctgatcagtg atttttccgt
      481 ttttcagttt tagttcgttg tatgtgcgtt tttgtgccgg acgatcacct ttttcaacat
      541 atttccggtt ctgaatggtc gaaatagtcg gagcataggt tgatggacgc ccgatgccaa
      601 gttcttcaag cttacgtacc agacttgcct cggtatatct tggcggacgc tgagtgaaac
      661 gttcctgagc catgatttcc gtggcagtca gattttcgcc ttttttcatc accggaagga
      721 ttgattcgtt ttcagcttcg gactcatcat ccgttgactc gagatacact ttcagaaatc
      781 cgtcgaaaac aatcacttcg ccactggcca caaactgata attaccgccg gatacattga
      841 tgtaaatgtt tgttttttcc agttctgcat cggccatctg cgaagcaatg gtacgtttcc
      901 atatcagatc gtagagtcgt ttttcctgcg cagtaccctc aatgctgtga gcgcttatgt
      961 aggtaggccg gatagcttcg tgagcttcct gagcgccttt ggttttagtg gtgaagttgc
     1021 gggtgtgtac gtatttctca ccggccatgc tgatgatttc gctttttgct gttccgagtg
     1081 ccagtgccga caaattaacg gagtctgtac gcatataggt tattttaccc gattcgtaaa
     1141 gtcgctgggc aaccatcatg gtctgcgaaa cagagaaacc aagcttccgg gcagcttcct
     1201 gctgcagggt cgaggttgtg aagggcggag caggcgattt ctttgctggt tttttttcga
     1261 tgctgtcaac attgaactgt gcgtttttgc ataattcaag gaatgccatt gcatcggctt
     1321 ttttatcgaa gcgttgtgat aattcggctt tcagttcaat gggtgtaccg tttgcatcgg
     1381 tcgatgaaaa aacggcggta accctgtaag ctgcttcggg agtaaacaga ttgatttccc
     1441 gttcgcgttc tacaatcagg cggactgcta ccgattgcac acgaccagct gaaaggcttg
     1501 ggcgaacttt gcgccagaga accggcgaca attcaaatcc cacgatgcga tcgagcacac
     1561 gacgtgcttg ttgtgcgtcc accagattca ggtctatttc gcgtggatgc tcaatggcat
     1621 gaagtatagc gtcttttgta atctcgtgaa aaactattcg tctggtgttt tcttttttca
     1681 gtttcagctc atcggcaagg tgccaggcaa tggcttctcc ttcgcggtcc tcatcggaag
     1741 ccagccaaac ggtagtcgct ttttttgctt ccttcttgag ttcagccaca attttctttt
     1801 tatccgccga aacttcgtat tgcggagcgt aattattctc aaaatcaata cccaatcctt
     1861 tctcgcccag atcccgaatg tgtcccatgc tggacattac ttcaaaatct tttccgagaa
     1921 acttttcaat cgtctttgcc ttcgcaggcg actctacaat gactaaatta ttggacataa
     1981 ttacgcttaa tttttaatta cgaattcaaa ttcgggtgca aaataacaaa aacaaaaggg
     2041 tatcagccaa attttcagcc tattattttt gttttttata aaattaagac aaaattagga
     2101 tcgtagatta caccgtaatt ttggttaatc gaggatgtca gttacttcgt aatcaatggc
     2161 ctctacggct tctttgatct ttttgtcgga tacttctttc gtcagtatca gtttagccga
     2221 tcggttttcc agatcaactt ttgcttcaac tccgtcaatt ttatttaatg ctttttcgac
     2281 ccgtgctgca cagtgcgagc agctcatacc tttgattatt gctgtttttt ccataattgt
     2341 ttgattttta tagttaataa ttatcgaata gtttgcgatt ttgtgggttt gaatttcttc
     2401 aatcttaacg cgttgcttac cacggtaacg gagcttaaac tcatggctgc cgcggcaaac
     2461 atcgggctga gtttccatcc aagtccggca taaaacacac cggcagctaa cggaatgccg
     2521 attatgttgt agaaaaacgc ccagaacaga ttttgtttga tatttctaat gacggcttta
     2581 ctcaaacgga tgccggttac cacaccgagc aagtcgtttt tcatcagcac tacatcggca
     2641 gcttcgatgg caatatccgt accgttacca atagcaaaac ccagatcaga ccgtatgagt
     2701 gcaggagaat catttattcc atcgccaacc atagcggtct tttcgcctgt tgcctgtaac
     2761 tgctgtacca ctttatcctt atcctgtggc agcacttcgg ctatcacctc gtccagcccg
     2821 agtttttcct gaattgccag agctgtacgc ttgttgtcgc ctgttaccat catagttttc
     2881 aggttcatcg atttaagcag ttcaatagcc tctttacttg tttgttttaa tacatctgct
     2941 actgcaataa tgcccagcac atttttctca tctgcaacta taagcggtgt tttacctttc
     3001 tgcgaatagt gatgtatctg attttcaatg tcaacagtat ttgtgttttc tgaaatgaaa
     3061 gacagattac cgacccgaac tattttatct ttaatttttc cggaaatgcc ttttcccgga
     3121 atattattaa aatcactaac gggatacaat tcaatttctt tttctgccgc tttctgaatg
     3181 attgctccgg atacagggtg ctccgaaaat ttttcgagtg atgcagtgat gcgaagcaat
     3241 tcatttttat ctaaataatg tatgggttcg atatctgtaa cttccggacg tccttcagtg
     3301 attgttccgg ttttatccag taaaacggta gttatttttc cacccgtttc aagtgcttcg
     3361 gccgatttaa acaacacgcc gcactctgcg cctttaccgg ttccgaccat gatagctacc
     3421 ggtgtagcca gccccagtgc gcacggacat gaaataatca gtaccgaaat accgatggac
     3481 aaggcaaatt caaaaggata gcccatgtta agccacacgg ctgtagccag tagggcaatg
     3541 ccaataaccg ccggaacaaa aatccggctt atctgatctg ccagtttgga aataggagct
     3601 ttcgaagaag atgcttcttc catcagctga atgatttgtg aaagcgtggt gtcttttcct
     3661 acttttgttg cacggaaggt gaagtaaccg ctcccgttaa tactggctga taatacatgg
     3721 tcgccttcct gtttgtaaac agggatgctt tcgccagtca gagccgactc atcaaccgac
     3781 cctgagccac cggtaagaac tccatcaacc gggatttttt gtccgggacg aacgcttacg
     3841 atatcgttta accgtacttc ttcaacagga atctctattt cagtgttgtt gcgaatgacc
     3901 gttgctgtct gaggtgctga gtcgattaat ttggtcaggg catcggaggt ttgacgttta
     3961 gattttgctt cgagatattt ccccagtgtt acgagtgtga gaatagtgga tgccgactcg
     4021 aaaaacaaat tgtgcaggta catcctgaca gtttcagggt tgttttgtcc aaggccgttt
     4081 ccgattttgt aaatggcaaa aaccccgtaa atgatagctg ccgaggaacc gattgctaca
     4141 agggcatcca ttgtaggtga cagtttccat aaacttcgaa aaccatgtga aaagaattta
     4201 ctgttgacaa aagcgatggg aagcgtgagc aaaaactgtg taaaagcaaa agtcagtgca
     4261 ttatcactgc ccgtgaaaac cgaaggaagc ggaaaatcaa gcatgtgtcc catacttaaa
     4321 tataacagcg gaactaaaaa caaaaatgag atgattactc ttcttttcat ctctttttgt
     4381 tcttcaagtg cataatttac ttttggagtt gtggtactta caatcgattt ttccttttca
     4441 aatgcttcat atccagcatc ttccaccgaa cctatgattt cttcaaccga caatgcctgt
     4501 tggtcgtaat caacagtcat actgtttgtt aagagattaa cgctcacatc ctggacaccg
     4561 tacagctttc gcacgctttt ctccacatga ttcacacagg ccgaacaggt cattcccacc
     4621 acatcaaatt tcttcgtttc catctttaat tattaatacg aattgaaaca atttaaatca
     4681 ggatatgttc atccttacat gtttctaaca gatttttagc agtaattaaa ggaaagacgc
     4741 cgaatttgat tatctgtacc agagttgttg atcccggcag gttttgcaga ccgcattttc
     4801 ataaggaatt cggtattcga gcatgatttc atgcgtgccg ttctgattgt aaccggtgat
     4861 gaagctctga tcgtaagcat aacctatcct gaaattttct gtcagattta ctcccagcaa
     4921 ggctgtaact tgccttgagc tccggtaggt aagtccaatg tcaaagattt cacggctgcc
     4981 ggttgataat ccggtggggt gtttgaaccg aacgatgcca ttaccttcca gtacaaaaat
     5041 attggaacgg ttgaccacct gaagtccaag atagtaattc atgatttctg aacgggtatt
     5101 tttgtaaatg gcaaagccat accggtgatt gtcgttcaga aacagattgc tgtttttgcc
     5161 gatagagaag aggtgagttg atgagatgcc gaatatgaaa ttatcgttct gaaattcaac
     5221 cccgatattt gcatcgggtt ttgtctgagg ttcaatatcg cgcaaaagag ccgggtcaag
     5281 gtcagttacc ggagcatagg atgcaaagtc aatggttcgg atatatgccc cgaccgaaac
     5341 tccaaacgat aaggaccagg tttgattgtc ggaaacgcga taggcgtaat taatcatcgg
     5401 gttgaaagca ttggtaagac cgatattgtc atttaccacg gataatccaa aggcagaatt
     5461 aattgaattg agatagccag atacctgtac attttccact tttggagctc ccgttatacc
     5521 tgtccactga ttacggatat tagagaaaag ataaatatag ccggttctgg cgatggaagc
     5581 cggattgtaa tttccccggt tataccagtg agttgccatg cttatatccg tttgagaata
     5641 agcacggatg ccagatataa tccagcaggt aagcaatatc catataacaa cgtttttgtt
     5701 cattctcggg ttattttttc agtgttataa atccctttat ttcttttaaa ctctgatttt
     5761 tatccttata attaatcaga tagaaatagg tgtcgttgtc tgctgtttta ccatgataag
     5821 taccatccca accggtgttt cctttgtaaa gtgaatttcc cgcgcggtca aaaacctgca
     5881 ggtcataacc agccatgaaa atatcgttca caccatcacc gttaggcgaa aacacattag
     5941 gtatataggg agcgacttct atcgtttttg ttgaaaaact gctacaacct gctaataggg
     6001 tggatttaag ctgaatttta tattcgtaag tgtttccgtt gattgggtag gtgtgaataa
     6061 aactgtttcc attctctatt gttccatctc ctaagtccca ttcataattt acaccatctt
     6121 cgggtgtaat gctgaaagtt acattgttgt ttttattatc aattttttct ctcgaaacag
     6181 aaaagtcact ttttggtact gtatttatca caacctttgt agctgcggtt gcaacctgtc
     6241 cgagcgcgtc ggtcaccgtt acattgtaaa tcccagacat acccgttgtg cagtttgaaa
     6301 ttaccggact tgactcttcc gaactaaatc cgttgggacc tgtccaagag taggtaaacg
     6361 gaagagcatc accagcggag ttaacaatca gttgcatgtt agatccttcg caaaccgggc
     6421 ttgtattcga tacgtctatg gaaagcggat ctgtgtgaag agtactgatc agaatatcgt
     6481 caatataata atcgttaccc attatcagtt tataactgtt taacaaacca attgtcacat
     6541 cgtttgtggc caatgttgct gatgtgggta ccgaaaaatt taagtcgtat ctgatccatt
     6601 taccttcatc agtcagtaat ggaccactgt aggtgtattt tttaccgttc gtgttgttaa
     6661 ttgcttccat cgaaaactgc gaacttttag caactacata aatccagaac gaaaagagat
     6721 aacgaaatcc ggtttttagt ttcagatcac gtttataaat atgatttgat gttaagccgg
     6781 cattgattac cattacagct ccgttatggt ctttttctgt gtgatcttcg gtaaaatatc
     6841 gcgtagaacc tgttgaagtt actgatgata gtgaaggtgc tgtccagaag taagaatcgc
     6901 ctgtcaaaat cgcatcttta atatgagcgg gatctaccac tgtgtaataa ttatcttcaa
     6961 tggttctctg tttgttgtct cctgtctcac tggcaaatgt gtaactcccg gcaggcacat
     7021 actgacattc aacacgatga atatgttctc cgaaattttc aaaaatgatt gtatcaccat
     7081 aggtcgttga ttgtgccttg gtagagattg tcggtgataa acaaacaagt actccgatta
     7141 gaaatataat tattttctgt tccataattt taaagggttt atctttttag ctgtataaac
     7201 ccttttgccg ttttttccga gccgtccgta tctatatatt ttatatagta aaaataggtg
     7261 tcattgtctg ctttttcccc tttgtaattg ccatcccatc cgttgtcgcc cttataaagg
     7321 atgttcccgt ttctgtcata tataatgatt tccttgccat ccataaattt gtcgttgatg
     7381 ccatcaccat tcggggtaaa aacattgggg atgaaaagca caacattgac catctttgag
     7441 gctgaattcc tgcaaccatt ggcattgact gcggtgaggg ttattttgta gttcgttaga
     7501 ttcgggtcta catcgtactt atgtgtaatt ctggttccct gttctgtttt gccgtcgccc
     7561 atatcccaat ggtagtttac tccgttctga gctgtaacgc tgcagctgag ttcattatta
     7621 tgtaaatcaa ttttgtttac agacatatta aaaccggcat cgggaagcgg atcttcattt
     7681 actgtaaatg tgaattgttt ttcgcagccg cgacggtttt tacctgttac ggtataactg
     7741 ccgggtttat cgaccctgat ggaatcggaa gtttcgcccg tgctccagct atatgtggct
     7801 gctcccgaag ccgaaagaac ggtgctgcct ccggagcaga aaagctcaga tccactggtt
     7861 gttaaagtcc aggctggctc cttttttaca ttaaccctga tagtgtccgt atggcagttg
     7921 ttgcttgaat atccgatcat ccacacctca gtgtcttttc ccacagtaat gttgtcggca
     7981 gtcgaaccgt cgaaccattc atagtgatga gctccgtggg ctttgagcaa tgttgtttcg
     8041 cccggacaat aggtggagtc accatcgatc ccaatcaacg gcggaaaaaa tgtttcaacc
     8101 attttggtca ctgaatccgt acaggtcgat ggtggatttt ttacaaccaa cgtaaccgga
     8161 atcattcccg aaactgagtg gaagtcatga attgggtttt gatcagtcgt tgtgctgcca
     8221 tcaccaaaat cccagtggta atcgagtgtt ccgtgtgtcg acggactggt aatggagaaa
     8281 atgatggtat tatttacttt gttacaatct acctgactgt gagtgaaatc ggcattgggc
     8341 gaatatctga atattgttgc cgaaagtgtg gcatcgcatc ctgttgccga ctgcatttcg
     8401 caggtatagg tgtcgccttc ggatattacg ttactcgaca gttggagcgt ttgtcctgta
     8461 aatacagggt tgttcaggct gtctttccat tgatacgatt taaatccgat aggagctgtg
     8521 agggtggcta ttttgtcgtc ggcacaataa tcaactgtaa tatataatgg ctgacaatgt
     8581 gctgtgaggt atgcataacc gaaatgccct tttcgacggc aattagccga cataaattca
     8641 agtgttactg tctgtccgat gtattttgta aggttaatgc ctactgtagt ccaatcgcgc
     8701 cagtataatg gtttaccatc gtattgtatt gtgttccatc cctccacatt ggcattcgaa
     8761 gcattgacat cgtagttggc gcaacttgct tccccggtaa tttcattacc atctttatcc
     8821 agaatactta ctttgaaacg gggttcatct tctggaaagt ggctgctgtc gtgcggatct
     8881 tgaagaacca ctgcaaagtt atatacaata aatgcgtttt gcggggtaac atccagctgg
     8941 taggttaggc tttgtcgcaa acctccatta cccaaactgg tagaaccaag ttttacggaa
     9001 gtagagtaac catccggtat gagtttcagt tgtgttccac ccactatcgg atcgtatccg
     9061 ttcgatgtga tgatggtatg tctgccgtta acaattcctt ctacttttgg tgtcgaagtg
     9121 acatatcccg attgaccatc tgcccaggta tatgctctcc agttggtaaa attatgaagt
     9181 tcaaatccta tatttgtgca ataaggttca tctgctaatc ctgtattcat aaatcctgta
     9241 agaaatacga ataacattat gatatgtttg tataggggtt tcacgcacaa atacatgaag
     9301 aagtatcaat ataaatgtat atattaagtc aaaaaaaatg taaagaaaac gataattatt
     9361 gttctatacg taatttttaa ctctatttat ttattttgat taagtttctg ataggaaaat
     9421 ctgatcggga aggactggct aatgttatgt ttttgaacgg gatggtttaa aattaaagat
     9481 ggtagccacg cctgactacc aatctttgtt aaccttaaat ctaatactat gaaaaaaatc
     9541 acgagtcaaa gatagaaatt gtttttttac cacacaagat tatctcttga ttttattcaa
     9601 ataataacat acattaacat agtatttaaa ataaaataca tatattaaaa cttaatgtaa
     9661 gttttacaat aaggttagtc tgattttcta tttttatgta gttacatata gttagttcta
     9721 tagacaatat aattgattta tattatcaat aatactcatg ggattttcaa gagtatttaa
     9781 ttctatttat ctcatttcaa tagattacgt gcttgattta tgcgtatcag atttcttcaa
     9841 atctggctgt gaaaaatcgc agttgttttg gttcataaat aaatcgcaga ccgcgtattt
     9901 gttctttgtt ttgataaagt ttgataaccg catcagccac caaatccata tgacgataag
     9961 tatatacacg tctgggtatc gtcagccgta ctgtttcaag tttagggcgg tgattttcac
    10021 ctgtaatttt gtcgcgtccg gccgatacta taccccgttc catactccga acacccgatt
    10081 cgaggtaaag attggccgcc aggctttgag ccggaaactg atcctgagtg aggtgcgggc
    10141 aaaaacgtcg ggcatccaga aatactgcat gtccacccac cggttcaata atcggaatgc
    10201 cggcttccag caagcggtcg ccaaggtaac gaacctgttt gatgcggtgt tcgatgtatt
    10261 catactgcat ggattctttc agaccgatag ccatcgcttc catgtcacga cccgccagtc
    10321 cgccataaga aggcattcct tcataaacta ccacgagttc tttggcatcc tcaaaaagct
    10381 tttcgtcgtt catgcacaga aatccaccga tatttaccag acagtctttt ttgccgctca
    10441 tggtgcatcc atcggcatag ctgaacattt catgtacgat ttcctttatg cttttatctt
    10501 taaattcttc ttcctgttct ttgataaaat aggcattttc tacgcagcgg gtggcatcat
    10561 agaatacttt tatgccgtgt ttacgggtga gttcgcgcac ttcgcgcatg ttttttatcg
    10621 atacgggctg gccaccagcc aggttcacag ttactgcaag acaaacgtaa gcaatcttat
    10681 cagccccttt atccattatc agtttttcga gttttttcag atcgatatct cccttaaacg
    10741 gaacattgag actagcgtca tgtgcttcat cgcggataat atccacaaat attcctccat
    10801 tcttttcctg atggaatcgg gtagtggtga aatacatgtt gcccggaaca tactgcccgg
    10861 gtttgatggc tatttgtgac aggatattct cagctgcccg tccctgatgt gtgggaacca
    10921 tgtatttaaa gccgaaatat tcctgtaccg tttggtagag gtggtaaaaa ttccggctgc
    10981 cggcatacgc ttcatcgccc atcattatac ccgcccactg acggtcgctc atggcgttgg
    11041 tgccgctatc ggtaagcagg tcaatgtaaa catcttccga atcgattaaa aaggtattga
    11101 atcccgcttt tttgatgaca gcttcgcgtt cttctcgtgt gttcattttt acatattcca
    11161 cactttttat gcggaaaggt tctgcgggat aattctgaaa gtccatattg atgtgttttt
    11221 tggttaatat tgcaattgct ttacaaatgt aaagtgaaat aagggaatat caaaagaaaa
    11281 atgtaaaata attttgaata aattcgtatg tggtgcgtat tgctgacaaa atgctatttt
    11341 atagttgtag tgcaatgaaa attgataaat aatttttgga cacgtttaaa tccgtaaagg
    11401 tcatcagaat cgtctgatat ataattattg cattttaaaa ccataaaaca aagtaatatt
    11461 tccctgtttt attcgtcaat aaatatgaac aggattcatc ttaaagaaat atttagttta
    11521 tcccatattt agttttgcat tttgtttaac aatccgcttg ccatgtgaat agtgagcgga
    11581 tttctttttg aattaataaa ttttttttgt tttttacaga tagtgtaata cgttgttatc
    11641 cagtaagtaa ataaatataa atgtgaggaa cgaagctgaa tggagaaatt ttttattgtt
    11701 tttaaatcca aaacgtgttt tgattcgtct aattcataaa caacgattat atttacaaac
    11761 caattaaact gaaagactaa aaatgaaacg attaggaatt ttcttattag gagtatttct
    11821 gctggtgggc agcatggcag ctcaggacca ggcgaaacag gatacggcaa aagctgtaac
    11881 gacagatagt ctcgaacagc tctatatgaa tgaagatctg gacgccagag aagcaatcct
    11941 gctgaaaaag ctggatgcaa aacagctgat ggatctcgaa acattgcgac tgaacgagca
    12001 gggtaaaaac aatatgccgt tttcgggatt aggattagta cttatttgtc tggcgccatt
    12061 tatcatggta attttaattg tatattttgc atcaagagag agtgcgaaaa aagccaaaca
    12121 gcgttacgat atctctatga aagcgcttga actcggacag cctctgcctc aaagcttttt
    12181 cgatgagccc aaaaagagaa cttcacgact gcaaagtggt ctggtatggc tcggggtggg
    12241 cattgcaatc gtaataggtt cctttgtttt aagtaaggaa aatttattct tgttcggaat
    12301 tataccggca tttgtcggac ttggaatact gattgcttat ctggttgaaa gaccaaaagc
    12361 cgacaacgcc aataacactc cggcaacgaa tgaataaagc tgaagatatg cttttggtat
    12421 cgcaggtggt tatcgctgat gaccaccgtg cgttcgatcg actggttcag aagtatcaat
    12481 cgcccatacg caggtttttg attaatctca ccatgggcga taccatgtta agcgatgatc
    12541 tggcacagga aacatttatc aaagcatatc tcaacattcg ctcttttcag ggcatttcgg
    12601 cattttctac ctggctgtat cgtattgctt acaatacttt ttacgactct gtgcgcgcta
    12661 agcggggcta cgatgatctg aatattgctg atctggacaa aaaatacagc accgaaatag
    12721 aatattctgc cgaaaaaaat gatatttttg cagcattgaa attgttgcgt caagatgaac
    12781 aggctgcaat tcttctttgt tatatggaag ataaaactca cagggaaatt gcaaaaatca
    12841 tgaacatccc acttggtact gtgaaaacaa acattctgaa gggaaaagaa aaaatgggaa
    12901 aatttttaat acaagcaggc tatggaaact aacgaagata aattattaaa atcttttttc
    12961 agccaaaaca aaccggaaat agcagataat gggtttaccc aaaaagtgat gcgccggttg
    13021 cctgaaaatg agcaaaaaac aggatggata attctaatgt ttacggcatt gggatgtctg
    13081 attacttttg cactggtaga tgtgcccgaa gtaatcagcg agatatttac tttcctgctt
    13141 ggagtaccca tttattacct gctgggtggc tttatgctca ttccaatagt aaccttggtg
    13201 gtgatgatag cttacagacg agaatataat ctggcctgaa taatcagttg aaataacaga
    13261 aaaaagtgga aaactcatac gggttttcca cttttattgt ttgaaagttt gttgtctgtt
    13321 atgcaaccgg atattaaatt cagctataac tcatcaatat cttttatctt tttttcaact
    13381 gccgcattcc agtcggaata aactttccgg taataatctt ttacgagctt cggattgttt
    13441 ttactgtcat aatgaccggc ccgggctctg aattctgtgg ccatcttcga aatatcaact
    13501 gccagaacgt cggctttttc ggatacatct tttttcgaaa gtaatgtttt aaaaaaaacg
    13561 tcagtgatta actctgatgc aatgaactgc attgtttttt tcagttttct gcggcttgcc
    13621 atgatgttat cagttttagg agtgaataat tgttacaaat ataactgata attatgatta
    13681 tagcgtcttt tttaagtaaa tttgccggaa aataatttat aacagtttac gttttcaaag
    13741 aataattcat cgcaaccgca tcctatgttt ttccgtaaaa tacatcatat actccttttt
    13801 ataataattg cacttgttgt acaaatgcta attccttcgt gtatagacga gagcatttcg
    13861 gccgatacaa gtcttaaact ggagttttcg gctgatacat tgcagttcga tacggttttt
    13921 acgaccatag gcagttctac ttcacagatc aaggtttata acccgaacaa aaagaatctg
    13981 aagatttcgg ctatcggact gggtatgggt gcaaactccc cctaccggat taatgtggat
    14041 ggcagcacca gcaagaataa ccagtttacc gacatagaac ttcgcgccgg cgacagcctg
    14101 tttgtttttg tggaagtgac catcgacccg cttaaaacaa atgcacctgt attcgtcaaa
    14161 gattctatcg tttttcttac aaactcgaac catcaggatg tgaaactcct tgcttacgga
    14221 caggatatgg agatactcag caacagaacc atccggaatg ataccacact gacgggtgaa
    14281 aaaccgtatt tggtatatgg cgatctggtg gtcgatagcg cgaaaacact tacactcaat
    14341 ccgggatgcc gtctgtattt tcataatcag gctagcctgg tggtttatgg caatctgatt
    14401 gccaatggta cccgcgaact tccggtgctg atgcgcggcg accgcacgga ccgtattttt
    14461 accgacattc cgtataatta tgtttcaaac cagtggggag gagtattgct gctgaatgaa
    14521 gcggggaaac ataaactcaa caatgtgacg atgaacagtg gttatgccgg agtttatctt
    14581 tccaatcaga accgcaacaa acgcccttca ctcgaaatga ctaactgtcg aattcacaac
    14641 tttctgaaat acggactggt ggtacagaac gccgatgtac tcgtggcaaa taccgaaatt
    14701 tcaaacaccg gagcttactc tgtttatctc aacgggggca aacatacttt tattcatacc
    14761 actattgcaa attatttcaa cagtaccaac gtacgcatac aaccctccgc acgcgaaaat
    14821 tatgctgcat tgatgatcat ggaactgaat cgtattattc ctatggaaac tgtttttgaa
    14881 aactgcatca tttcaggttc ttcgaccaat gagatggata tcatgacccg gttttcggaa
    14941 cagtatcacg gattattcag caactgttac atccggaaaa ccaaaccggc caatgaaaat
    15001 cctgtttaca gccacatcag ctggtacgac aagaataaaa gagatacagt gtttgtaaat
    15061 acttattttg acaacgaaaa actggtttac tataacttca tgcctgattc tgtgtcgcca
    15121 gcccgaaaca ttggcagtat ggatgcagcc ttaaaatatc cgtttgatat aaatggaaac
    15181 agcagacttg aagataataa gcccgacgca ggggctatcg aatggcaacc ggtaaaaaaa
    15241 acagcaaaat agtctgaaat tcaaagtatt gtacatgaaa aagcttagtg tcatcctatt
    15301 tatatttgtt gtttgtcttt cggcgaaagc acagggacat gaaaagcaga atttcaggat
    15361 tgtgagctat aatgtggaga actatttcga cgtggtaaac gactcgctga ccattgatga
    15421 agaatacctt cctgaaggtc tgcgtgcatg gaacattaca aagtatcaga caaaacagaa
    15481 caatatttca aaggttatag cagcaattgg gggatgggat gcaccagctc tggtggctct
    15541 ctgtgaaatt gaatcagaaa aatgtatgcg ggaactaacc gggttttctc ccttgaaaaa
    15601 tctgggatat aaatatctgc atcatgaatc acccgatgct cgcggagtgg atgttgcgct
    15661 gatgtatcag ccggatatgt ttgttccttt tcacgatgaa gccatacgca tcaatttccc
    15721 tgaaaccaat tctacaaccc gtgatatttt atttgcttcc ggattgttgc caaacaacga
    15781 taccttgcat gttttcgtat gtcatttccc gtcgcggctg ggaggtgaac tggaatcgga
    15841 agaccgccg
//
LOCUS       DAOPYB010000055        15813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_3970_length_15813_cov_8.738165, whole genome shotgun sequence.
ACCESSION   DAOPYB010000055 DAOPYB010000000
VERSION     DAOPYB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15813
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_15813_cov_8.738165"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /gene="ettA"
                     /locus_tag="PKH58_05970"
     CDS             complement(<1..1586)
                     /gene="ettA"
                     /locus_tag="PKH58_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNX88608.1"
                     /translation="MADDKKVIFSMVGVSKIFPPQKKVLNNIYLSFFYGAKIGIIGLN
                     GSGKSTLMKIIAGVEKSYLGDVVFSPGYTIGYLEQEPMLDPDKTVKEVVQEGTQEVVD
                     MLAEFDRINEQFADPDADFDKLIARQGELQELLDHADAWNLDNKLDRAMDALRCPPED
                     ALIKNLSGGERRRVALCRLLLRQPDILLLDEPTNHLDAESVEWLEHHLHEYAGTVIAI
                     THDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKTTRMAMEEKQASKRRKTLEREL
                     EWVRMAPKARQAKGKARLEAYDRLLNEDQKEREEKLELFIPNGPRLGNKVVEAIDVSK
                     AYGDKLLFENLNFMLPPNGIVGIIGPNGAGKTTLFRLMMGLEKADKGTFDVGETVKIG
                     YVDQSHTEIDPQKTVYQVISGGTEFIRVGGKEINARAYLSRFNFTGADQEKLCGVLSG
                     GERNRLHLSMTLKSEANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVVISHDRWFLD
                     RICTHIIAFEGDSQVFVFQGG"
     gene            1702..2073
                     /locus_tag="PKH58_05975"
     CDS             1702..2073
                     /locus_tag="PKH58_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNX88609.1"
                     /translation="MKKIILIILGSISLGLAILGVFLPLLPTTPLLLLSAALYARSSE
                     KLYNWLLNHPVFGEYIRNFREDKAIPLNIKIIAISTLWLFMLYSIFFIVNKKWYLQVL
                     LAGIALGVSIYILSFKTKQKK"
     gene            complement(2088..3254)
                     /locus_tag="PKH58_05980"
     CDS             complement(2088..3254)
                     /locus_tag="PKH58_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88610.1"
                     /translation="MDELKKQIKDSFYNPILYFLPLLFFIVMDDFYGLNMAWKISFPV
                     ALLLIFYVYWFYNRLFLWHLLLSVGYILIGLIVSLIPEEPESKEIFQYSDEALFILFI
                     LILIISKSSLEKIAAKTLSYEMPMSNNVNELFRFNNNLLAIVSLYLIVIVLEAFGVFS
                     FTREQVQNLHYAYLLAFLFLGVFETVRVFIIRYRLLHEDWLPIINDEGKVTGSSQYFH
                     ELPHKKHMHPVVRLHFIDNGILYLQQRKADDYDEASLWDAPVSRHVRLGESIYQSLKY
                     KIQKLYNIEPLKFLFLTNYKYENSTEYQYVYLFVLCKTDGLKPNMNKIQATKWWTQNQ
                     IEDNLTTSIFTERFKIEYELLKRTGLLEQESCDCDCVLKNLINNRINRDVTRAD"
     gene            complement(3376..3981)
                     /locus_tag="PKH58_05985"
     CDS             complement(3376..3981)
                     /locus_tag="PKH58_05985"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNX88611.1"
                     /translation="MFSGIVEEAATVVALVQEQENLHLTMNCSFVNELKIDQSVSHNG
                     VCLTVVSKTADTYTVTAIKETLNCSNLGKLRIGDKVNVERSMMMNGRLDGHIVQGHVD
                     QTAICTAIDEAEGSWYFTFEYEFDPAMARKGYMTVDKGSVTVNGVSLTVCNPTNNSFQ
                     VAIIPFTFEHTNFGQIEVGTYVNIEFDIIGKYISRMLEFNK"
     gene            complement(4040..4981)
                     /locus_tag="PKH58_05990"
     CDS             complement(4040..4981)
                     /locus_tag="PKH58_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNX88612.1"
                     /translation="MAKYPDFLKEGDEVRIVSPAGAIEPVFVDGAIITLTSWGFKATE
                     GMFTRHALGRYAGNEQERYTDLQQALDDPRVKAILCSRGGYGLAQIIDRIDFSRFLKN
                     PKWVIGFSDITILHQAILSTGFISIHGVMAKQLTELPAHSEALLLLENILKGKTPSYK
                     IIPDAKNRQGTAKGKLIGGNLSVFMGMRGTKFDLSFKNSILFIEDVGEKPYQVDRMLQ
                     NLRISGALSQLSGLLVGQFTEYDEDPDMKKSLHQIIADAVKDYDYPVCYNFSAGHIDQ
                     NFPLLMGATAELSVEQNGAGLTFQTTFWKKAEKLLNL"
     gene            complement(5015..6265)
                     /locus_tag="PKH58_05995"
     CDS             complement(5015..6265)
                     /locus_tag="PKH58_05995"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX88613.1"
                     /translation="MRFDELPLCDAVLDGLQAMNFTEMTPVQEQAIPVILQKRDLIAC
                     AQTGTGKTAAYILPLLNNLQTTPHTDDAVNAIIMSPTRELAQQIDQQMEGFSYFVPTS
                     SVAVYGGNDSRAWDVQRNGLQKGADVVIATPGRLISHLNLSEIDLSHVRYFILDEADR
                     MLDMGFFDDIMQIVNRLPKERQTIMFSATMPPKIRQLAKTILKDPVEVKIAISRPPES
                     IMQTAYICEEAQKNGIIRNLFAEKSPEKVILFSGSKLKVKEITKTLRKMGLSVGEMHS
                     DLDQAQRDSIMHEFRNNRVNMLIATDIVARGIDIDDITLVINYDVPWEVEDYVHRIGR
                     TARAGENGMSITLVAPEEQYRFKQIEDFLEKDIFKIPLPPELGEAPEYNPDANRRKKK
                     QFSKPKGNFKRNKNRNKDKKIQDK"
     gene            6544..8025
                     /gene="proS"
                     /locus_tag="PKH58_06000"
     CDS             6544..8025
                     /gene="proS"
                     /locus_tag="PKH58_06000"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNX88614.1"
                     /translation="MAKEIKELTSRADDYSQWYNDLVIKADLAESSAVRGCMVIKPYG
                     YAIWEKIQAELDKMFKETGHQNAYFPLFIPKSFLSREAEHVEGFAKECAVVTHHRLKQ
                     NPNGSGLIADPEAKLEEELIVRPTSETIIWNTYKGWIQSYRDLPILVNQWANVVRWEM
                     RTRLFLRTAEFLWQEGHTAHATREEAIEEAEKMLHVYADFAENFMAVPVLRGVKSPNE
                     RFAGAIETYCIEAMMQDGKALQAGTSHFLGQNFAKAFDVTFVNKENQQDYVWATSWGV
                     STRLMGALIMTHSDDNGLVLPPNLAPFQVVIVPIYKNEEQLNAISEKVKEITAKLKAL
                     GISVKYDDNDTKKPGWKFAEYELKGVPVRLAMGARDLENGTVEVARRDTLTKETIAVD
                     GVELYICKLLKEIQQNIYKKAFDYRASVTREVNSYEEFKVEIEKGGFLLCHWDGTAET
                     EEFIKGDTKATIRCIPLDGDKTPGKCMVTGKPSAQRVVFARAY"
     gene            8106..9065
                     /locus_tag="PKH58_06005"
     CDS             8106..9065
                     /locus_tag="PKH58_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX88615.1"
                     /translation="MVWKDFLYFSKGQRTGILILISLIILLLVSTFLMPYLIRNEKDE
                     VEDITFLKEAEKFRASLVEKKRNKTYPYDNYPEYHKSYPHKTYNSKYQLFTFDPNTAD
                     SATLVRLGLKSYIARNIIKYRGKGGKFRKPEDFRKVYGISSEKADELLPYIQIAEQKP
                     KEITFSASKSNNELSEKHESDYSAKKNVIVELNSADTTTLMQIRGIGRRYAKSIVGYR
                     RILGGYYSVEQIREVYGMRPETFEQIKPFLNTDPSLIAKIDVNKASIERLKSHPYLNF
                     SKAKEIYELRREKGKLKKLDELQNLHSLTPEDLQKIGHYLNFE"
     gene            9104..>9616
                     /locus_tag="PKH58_06010"
     CDS             9104..>9616
                     /locus_tag="PKH58_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX88616.1"
                     /translation="MKFLENEKILLRALEPEDLDLLYSWENDSTIWIAGNTRVPYSRF
                     QLKNYIAEAPSDIYESKQIRLMIVDKSDNSTVGTVDLFDFDIHNSRIALGLFIADTHT
                     GRGFAKGSLKLVEEYVFNFLKINQLYVQIIESNHASRKLFENNFELHGKLKNWIKTPD
                     KYEDVLTYQKF"
     assembly_gap    9617..9716
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9717..10206
                     /locus_tag="PKH58_06015"
     CDS             <9717..10206
                     /locus_tag="PKH58_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88617.1"
                     /translation="KHNFYNSEAMEIYINLVDDTKKHLTHIAIGVVFILVGLIYMMMK
                     IRDKSFDQFDWAYLGFYGIIGFKFLIQGLIEILRKAYVVINNEKIALKPDTTTKGKTI
                     FWKDILTIKRVGKNYEILCSDKTSYTIYLSHYSYKVADEIKIALETIAAEKAILIETL
                     TN"
     gene            complement(10289..13495)
                     /gene="carB"
                     /locus_tag="PKH58_06020"
     CDS             complement(10289..13495)
                     /gene="carB"
                     /locus_tag="PKH58_06020"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HNX88618.1"
                     /translation="MKKVLVLGSGALKIGEAGEFDYSGSQALKALREEGVQTVLINPN
                     IATVQTSEGIADKVYFLPVTPDFVEQVIEKEHPDGIFLSFGGQTALNCGVALYRNKIL
                     EKYNVKVLGTPVQSIIDTEDREIFNRKLSEIGVKYIKSAAVTSLDDALRAANSLGYPV
                     IVRAAYALGGLGSGFCDNDTELKTLVDKAFSYSPQVLVEKSLKGWKEIEYEVVRDRYD
                     NCITVCNMENFDPLGIHTGESIVVAPSQTLTNSEYYKLRELSIRIIRHIGIVGECNVQ
                     YAFDPVSEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLGLGYGLTELKNSITKVT
                     SAFFEPSLDYVVCKIPRWDLGKFHGVSRELGSSMKSVGEIMSIGRNFEEVLQKGLRMV
                     GLGMHGFVGNKEFETGNVEKDLSVPTDRRIFFLSQAFHEGYNVDKIHELTKIDKWFLY
                     KLQHIVDLEKELASYHKLSDLPDEFLLEAKKKGFSDFQITRIVVKSKSHDMDDETKKT
                     REYRKSRGIVPVVKQIDTLAAEYPALTNYLYVTYNGNATDVHYLGDKRSVVVLGSGAY
                     RIGSSVEFDWCGVNALNTIRQQGLRSVMINYNPETVSTDYDMCDRLYFDELTYERVLD
                     ILEAENPMGTIVSTGGQIPNNLAMKLAAANMRILGTQAKDIDRAEDRNKFSAMLDELR
                     IDQPRWQELTTYSDVNKFVRDIGFPVLVRPSYVLSGAAMNVCYDETQLKAFLDLATEV
                     SKKHPVVISEFLERCKEIEIDAVADKGEIVVYAISEHVEYAGVHSGDATTQFPPQKIY
                     IETIRRIKNIAREIASSLNISGPFNIQFLARDNYIKVIECNLRSSRSFPFVSKVLKIN
                     FIDLATKVMLGIPVEKPEKSAFELDYVGIKASQFSFTRLQHADPVLSVDMTSTGEVGC
                     IGDSFSEAILKSLLSVGYRIPQKRIMISSGTTKSKVDLLDACKMLDEKGYELYATKGT
                     QKFLADNDIKSIAVNWPDEEGDYNIKEMIAEKKFDMIINIPKDLSRRELTNGYIIRRS
                     AIDFNIPLVTNARLASAFIRAFCTLKKEDIKIKSWDEY"
     gene            complement(13595..14656)
                     /gene="carA"
                     /locus_tag="PKH58_06025"
                     /pseudo
     CDS             complement(13595..14656)
                     /gene="carA"
                     /locus_tag="PKH58_06025"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
     gene            14945..15637
                     /locus_tag="PKH58_06030"
     CDS             14945..15637
                     /locus_tag="PKH58_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88619.1"
                     /translation="MKSFKFVFIVIFTFSVLSVFAQIETSAPILSEKFLVESYEKKLN
                     TKNVISYEDIKGTPYWKKEYIEGKLYLNDTVVVQAPLRYNIYTDEIEYKSNDRNYAIG
                     NPAYLKKALIGKTTFLYIPFIGNGGYYELLESGKCYLLQRRTMRFKPVEYAKPIIGTV
                     PAEFIPEPDVFYLVVDNNRWYKIVSLKSVKNVMLDQEKMIDEFISREKIRSIKKENLI
                     KIVKHYNSFVNN"
BASE COUNT         4675 a         3287 c         3005 g         4746 t
ORIGIN      
        1 cctccctgga atacaaatac ctgtgaatcg ccttcgaagg caatgatatg ggtacagatg
       61 cggtctagga accaacggtc gtgactgata acaactgcgc aaccggcaaa attttccaga
      121 ccttcttcca acgcccggag tgtatttaca tcaatatcgt tggtcggctc gtcaagcaat
      181 aacacattgg cttccgactt aagcgtcatt gacagatgaa gtctgtttct ttctccaccg
      241 gatagtactc cgcaaagttt ttcctgatcg gctccggtaa aattaaaacg cgagagataa
      301 gcacgggcgt tgatttcttt tccgcctaca cggataaact ctgtgccacc cgatattacc
      361 tgatagacgg tcttctgagg atcaatttcg gtatgacttt gatcaacgta accgattttt
      421 acggtttcac cgacatcaaa agttcctttg tcggcttttt ccaatcccat catcaggcgg
      481 aaaagcgtgg tttttcctgc accattggga ccgattattc cgacaatgcc atttgggggc
      541 agcataaaat tcaggttttc gaacaagagc ttgtcgccat atgcctttga aacatcgata
      601 gcttccacca ctttatttcc aagcctgggt ccattaggga taaacaattc cagcttttct
      661 tcccgttctt tctggtcttc gttcaacaac ctgtcatatg cttccagacg tgccttacct
      721 tttgcctggc gggcttttgg agccatcctc acccactcca actcgcgttc cagggttttc
      781 cttcgtttgc tggcttgttt ttcttccatt gccatacgag tagttttttg ttccagccac
      841 gatgaataat tacccttcca gggaatgcct tcaccacgat cgagttcaag aatccaaccc
      901 gctacattat ccagaaaata tctgtcgtgt gtaatggcaa tgactgttcc ggcatattca
      961 tgcaggtgat gttcaagcca ttccaccgat tctgcatcga ggtgatttgt aggttcatcg
     1021 agtagaagaa tatcgggttg gcgcagcaac aaacgacaca gtgctaccct gcgacgttca
     1081 cctcctgaaa ggtttttaat gagtgcatct tcaggcggac atcggagcgc gtccatcgca
     1141 cggtcgagct tattgtccag gttccaggca tcagcatgat ccagtaattc ctgcaactcc
     1201 ccctgacggg caatcagttt gtcgaaatcg gcatcggggt cggcaaactg ttcgttgatg
     1261 cgatcaaatt cggcgagcat gtcaaccact tcctgcgtac cttcctgcac aacttccttc
     1321 accgttttgt cgggatcaag catcggttcc tgttcgagat atccaatcgt ataaccgggt
     1381 gaaaaaacga catcacccaa ataggatttt tcgactccgg caatgatttt catcaacgtg
     1441 gattttcctg aaccattcaa tccgatgata ccaatcttcg ccccgtaaaa gaaagagaga
     1501 tagatattat tcaacacttt tttttgtggg ggaaatatct tgctcacacc caccatggaa
     1561 aaaatcactt ttttatcgtc agccataaac tattgaacta gtgtattatt tattgttttt
     1621 tttattgaaa ttatagaaat gcaaagataa aataaaaagg aacgcaaaac aatttgtaat
     1681 tttcataact ttgtaccctc aatgaaaaag ataatcctaa tcatacttgg ttctatcagc
     1741 ctgggtttag ctatactggg tgtattctta ccattgctcc ccaccacacc tttactgttg
     1801 ctatccgcag ctctgtatgc gcgcagctct gaaaagcttt acaattggtt attgaatcat
     1861 cctgtattcg gagaatatat acgcaatttc agagaagata aagcgatacc attgaatata
     1921 aaaattatag ctatttctac cttgtggctg tttatgctgt atagtatatt tttcattgtg
     1981 aacaaaaagt ggtacctgca agtactgttg gcaggtattg ctttgggggt aagtatttat
     2041 attttgtcat tcaagacaaa acaaaagaag taaattcatt agacctgtta gtcagccctg
     2101 gttacatccc ggtttatcct gttattaatc aaattcttta acacacaatc gcaatcacaa
     2161 ctttcctgtt caagtaatcc tgttcttttc aataattcat attcaatttt aaatctttcc
     2221 gtaaaaatac ttgttgtcag attatcttcg atctgattct gcgtccacca tttagtggcc
     2281 tgaattttat tcatgttggg tttcagacca tcggttttac ataacacaaa aagataaacg
     2341 tactgatatt ctgttgaatt ctcatatttg taattggtca agaataaaaa cttcagaggt
     2401 tcaatattgt atagtttttg tattttatac ttcaggctct ggtaaattga ttcacccaat
     2461 cgtacatgcc ggcttacagg cgcatcccat agtgaagctt cgtcgtaatc atctgctttt
     2521 cgttgttgca gatacaaaat accgttatca ataaagtgaa gtctgacaac aggatgcata
     2581 tgttttttgt gaggcagttc atgaaaatac tgactgcttc ctgttacttt tccttcgtca
     2641 tttataatcg gcagccagtc ttcatgcaat aatctataac gaattataaa tacacggacc
     2701 gtttcgaaaa cacccaggaa taaaaacgcc aaaagatagg cataatgtaa attctgtact
     2761 tgttctctgg taaagctaaa tactccgaaa gcttctaaaa cgataactat cagatatagt
     2821 gaaacaatag cgagaagatt attattaaaa cggaatagtt cattaacatt attagacatt
     2881 ggcatttcgt aagacaaagt cttggcggca attttttcca gagacgactt agatatgatc
     2941 aggataagga taaacagaat aaacagcgct tcatcggagt attgaaatat ctctttagat
     3001 tctggttctt ctggtatcag acttacaata agtcctatta gaatataacc cacagaaagc
     3061 aataaatgcc acaaaaaaag tctgttataa aaccagtaaa cataaaatat cagtaataaa
     3121 gcgaccggaa acgaaatttt ccatgccata tttaacccat aaaaatcatc cattactata
     3181 aaaaacagca atggcaaaaa atagagtatc ggattataaa acgaatcttt tatctgcttc
     3241 tttaattcat ccatgtcatc agtatcaatc agcgtgataa tagtaatctg tggaaatatg
     3301 aaatcctcaa tttatctgta aaacaaaata aattgaggat agttcaaaga aaatcctaat
     3361 agactaaatt tcctattatt tattgaattc aagcatcctg ctgatgtatt ttccgatgat
     3421 gtcaaattcg atatttacat acgttccaac ttcaatttgt ccaaagttag tatgttcaaa
     3481 tgtaaaagga ataattgcca cctgaaaact gttgttagtc ggattgcata ctgtcagact
     3541 aactccgttg acagtaacag atcctttatc aacagtcata tatcctttac gtgccatagc
     3601 tgggtcgaac tcatattcaa atgtaaaata ccagctaccc tccgcttcat caatggcggt
     3661 acaaatcgca gtttgatcga catgaccctg aactatgtgt ccatccagac gaccattcat
     3721 catcatactc cgttcgacat tcactttatc accaatcctg agttttccca gattactgca
     3781 gttaagcgtt tcttttattg ccgtaaccgt atatgtatct gccgttttac ttacaacagt
     3841 aaggcatacg ccgttatgcg aaacgctctg atctatcttt aattcattta caaaggaaca
     3901 attcatcgtc agatgcaggt tttcctgttc ctgcaccaaa gctacaacag tagccgcttc
     3961 ttctactata cctgaaaaca tattctattt attttattat tagtttctta tatcgtatgt
     4021 tatgcttctt attcttcatt tacaaattca gaagtttttc cgcttttttc cagaatgtgg
     4081 tctgaaatgt aagtccggca ccattctgtt cgacagacaa ttcagcggtt gctcccatca
     4141 acaatggaaa attctgatcg atatgtcctg ccgaaaaatt ataacacacc ggataatcat
     4201 aatcttttac cgcgtcggca ataatctgat gcagcgattt tttcatatcc ggatcttcgt
     4261 catattccgt aaactgaccg acgagtaaac ctgacaactg tgacaatgct ccgctaatcc
     4321 ggagattttg cagcatgcgg tcaacctgat aaggtttttc acccacatct tcaatgaaca
     4381 aaatagaatt tttgaatgat aaatcaaatt ttgttccacg cattcccata aaaacggaca
     4441 aattccctcc tatcaatttt ccttttgctg ttccttgtct gtttttcgca tccggaatga
     4501 ttttataaga aggtgttttt cctttcagta tattttcaag caacaagagc gcttccgaat
     4561 gagccggaag ttcggtcagt tgctttgcca tgacgccatg aatggaaata aaacctgttg
     4621 agagtatagc ctgatgcagg atggtaatat cgctgaagcc tatgacccat tttggatttt
     4681 tgagaaaacg gctgaaatca atccggtcga tgatttgagc cagtccgtat ccgcctctgc
     4741 tacacagaat tgctttaact cgtggatcgt caagtgcctg ctgtaagtct gtgtaccgtt
     4801 cctgttcgtt gcccgcataa cgtccgagcg catgtcgtgt aaacatgcct tcagtagcct
     4861 taaagcccca ggatgtaagt gtaataatgg ctccgtcaac aaaaaccggt tcgatggctc
     4921 ctgccggtga aacaattctg acctcgtcgc cttccttcag aaaatcggga tattttgcca
     4981 tatatttaat cgttcaaatt ttatataaca gggattattt atcctgaatc tttttatctt
     5041 tattcctgtt cttatttctt ttgaaattac ctttgggttt actgaattgt tttttctttc
     5101 gcctgttggc atccgggtta tactcgggag cttcacccaa ttcgggtggc agcggaattt
     5161 tgaaaatatc tttttccaga aaatcctcta tctgtttgaa gcggtattgt tcctcaggtg
     5221 caaccagtgt gattgacatt ccgttttctc cggcacgtgc cgtacgaccg atgcgatgaa
     5281 cataatcttc cacttcccag ggcacatcat aattgattac cagcgtaata tcatcaatgt
     5341 caattccccg cgcaacaata tcggtagcta tgagcatatt cacccggttg ttgcggaatt
     5401 cgtgcataat gctgtcgcgc tgagcctgat ctaggtcgga gtgcatttca cctaccgata
     5461 aacccatttt ccggagcgtt ttggtgattt cctttacttt cagttttgaa cctgaaaata
     5521 gaataacttt ctcaggtgac ttttcggcaa aaagatttct gatgataccg tttttctgtg
     5581 cttcctcgca aatgtaggct gtctgcatga tgctttcagg cggacgggag atggctattt
     5641 ttacttcgac cggatctttc agaatggttt tagccaattg cctgattttc gggggcatgg
     5701 tagccgaaaa catgatggtt tgtcgttctt tcggcagtcg gttgactatc tgcatgatat
     5761 cgtcgaagaa acccatatcg agcattctgt cagcttcatc caaaataaag taacgcacat
     5821 gtgaaaggtc aatttctgaa agattcaaat gtgatatcaa cctgcccgga gtggcaatta
     5881 ccacatcggc acctttttgc agtccgttac gctgaacatc ccaggcgcgt gaatcattgc
     5941 caccataaac agcaaccgac gatgtgggta caaaatatga aaatccctcc atttgctgat
     6001 cgatctgttg tgccagctcc cgggttggcg acatgataat tgcattaaca gcatcatcgg
     6061 tatgtggtgt tgtttgcaaa ttattcagaa gcggaagtat ataagctgct gttttgcctg
     6121 taccagtttg tgcgcaagct atcaaatctc ttttttgcag tattaccggt attgcctgct
     6181 cctggacagg tgtcatttcg gtaaagttca ttgcctgcaa gccatctaaa acagcatcac
     6241 aaagcggtaa ttcgtcaaat ctcatttagt tactttttca tttactattc ttatacaaaa
     6301 gtacggattt taattcaatt ttcagtagta aacgttttcc aattaataat ggttcgtaaa
     6361 atttcaatgg tttgtctttt tgaaccaagc ataaattttc cgtgtaactt tttcgttcga
     6421 atcatgtaca ccgaatatta attcgtcttg acattgacat attttcctat ttgaaaatac
     6481 tttaaaaatt catatctttg caattcattt taaatatagt aatacaagac atttcaatat
     6541 attatggcaa aagaaattaa agaacttact tcccgcgcag atgattactc acaatggtat
     6601 aatgacctcg taataaaagc cgatctggct gaatcctctg cagttcgtgg ttgtatggtc
     6661 attaaaccgt atggttatgc catctgggaa aagattcagg ccgaactcga taaaatgttc
     6721 aaggaaaccg gacaccagaa tgcatatttt ccacttttta taccaaaatc ctttttaagc
     6781 cgtgaagcag aacacgtgga gggttttgcc aaagaatgtg ccgttgtaac tcatcatcga
     6841 ctgaaacaaa atccgaacgg aagtggtctg attgcagatc cggaagccaa acttgaagaa
     6901 gaacttatcg ttcgtccgac atcagaaacc attatatgga atacctacaa aggttggata
     6961 cagtcgtatc gcgaccttcc tatcctggtt aatcagtggg caaatgtggt tcgctgggaa
     7021 atgcgtaccc gtttattcct ccgtactgca gaatttttgt ggcaggaagg tcacacagct
     7081 catgccaccc gcgaagaggc aatagaagag gcagaaaaaa tgcttcatgt ttatgctgat
     7141 tttgcggaga attttatggc agtacctgta cttcgtggag taaaatcgcc taacgaacgt
     7201 tttgccggtg cgattgaaac atattgtatt gaagcaatga tgcaggatgg aaaagcactg
     7261 caggctggaa cgtcacattt cctcggacag aattttgcaa aagcatttga tgtgacattt
     7321 gtcaacaaag aaaaccaaca ggattatgtt tgggctactt cctggggagt ttccacccgc
     7381 ctgatgggtg cgctgattat gacacattcc gacgacaacg gtttggtttt gcctcctaac
     7441 ctggccccgt tccaggtagt aattgttcct atttataaaa atgaagaaca attgaatgct
     7501 atcagcgaaa aggtaaaaga aattactgca aaactaaaag cactgggcat atcggttaaa
     7561 tacgatgata atgacacaaa aaaaccaggc tggaaatttg ccgaatatga actgaaaggt
     7621 gtaccggttc gcctggcaat gggtgctcgt gatctcgaaa acggcactgt tgaagttgcg
     7681 cgtcgcgaca cgttgacaaa ggaaacgata gctgtagatg gtgttgaact gtacatctgc
     7741 aagctgttga aagaaataca gcaaaatatt tataaaaaag cgtttgacta ccgcgcttcg
     7801 gttacgcgtg aagtgaattc ctacgaagag tttaaagtgg aaattgaaaa aggcggattt
     7861 ttactttgtc actgggacgg cactgccgaa acagaagaat ttatcaaggg agataccaaa
     7921 gccaccatcc gctgcattcc gcttgatggt gacaaaacac cgggtaaatg tatggttacc
     7981 gggaaacctt cggcacaacg cgtagtattt gcaagagcat actaaaaaca ttcgtttaac
     8041 aggtaaatta ttaattctca gcaaataata atttacctgt tattcaatta accaaataat
     8101 ctgttatggt ttggaaagat ttcttgtatt tttccaaagg tcaacgtacc ggtattttaa
     8161 tattgatttc gttgattata cttctgcttg tttcaacttt tctaatgcct tatttgatta
     8221 gaaacgagaa agatgaagtt gaagacatca cttttttaaa agaagctgaa aaattcaggg
     8281 caagccttgt tgaaaaaaaa cggaacaaaa cctatccata cgataattac ccagaatatc
     8341 acaaatcata tcctcataaa acatataaca gcaaatacca gctgtttact tttgatccaa
     8401 atactgctga ttctgctaca ttagtacgtt tgggactaaa atcctatatt gccagaaata
     8461 tcattaagta ccgcggtaaa ggtggtaaat tcagaaaacc ggaagatttc agaaaagtgt
     8521 atggtatcag tagtgaaaaa gccgatgaac ttttgccata tattcagatt gccgaacaaa
     8581 aaccgaaaga gataacattt tctgcttcaa aatcaaataa cgagttatct gaaaaacacg
     8641 aaagtgatta ttctgctaag aaaaatgtaa ttgtagagtt aaattctgct gatactacca
     8701 ccttaatgca aatacgcgga ataggcagac ggtatgctaa aagtattgtg ggttatcgca
     8761 gaattttagg tggttattat tcagtagaac aaatcaggga agtgtacggt atgagacctg
     8821 aaactttcga gcagattaaa ccgttcctta acacagatcc ttctcttatt gcaaaaattg
     8881 acgttaacaa agcgagcatc gaacgactga aaagtcatcc ctatcttaac ttcagtaaag
     8941 caaaggagat atatgagtta cgcagagaaa aagggaaact taagaaactg gacgaactgc
     9001 aaaatttgca ttcccttaca cccgaagacc tgcaaaaaat aggtcactac ctgaattttg
     9061 aataaagaat atcatttttg ataagttaaa aggataaaaa cgaatgaagt ttcttgaaaa
     9121 cgagaagata ttgttgaggg ctttggagcc agaagacctg gatttgctat atagctggga
     9181 aaacgacagt acaatctgga tagcaggtaa tactcgcgtt ccgtattcac ggtttcagct
     9241 gaaaaattat atagctgaag caccttcaga tatctatgaa agcaaacaaa tccggttgat
     9301 gattgtggac aagagtgata atagtactgt aggaacagtt gatttgtttg attttgacat
     9361 tcacaacagc cggattgcgc ttggactttt tattgccgat acacatacag gcaggggttt
     9421 tgccaaagga agtctgaaac ttgtagagga atatgtattt aactttttga aaataaatca
     9481 actctatgtt cagattatcg aaagtaacca cgccagccga aaacttttcg aaaacaattt
     9541 tgagttgcat ggcaaactaa aaaactggat caagacaccc gacaagtatg aagatgttct
     9601 gacctatcag aagtttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa
     9721 cacaatttct ataattcaga agccatggaa atatatatta atttagtgga tgataccaaa
     9781 aagcacctga ctcatattgc catcggggtt gtatttattt tggttggctt aatatatatg
     9841 atgatgaaaa taagggacaa atcctttgat cagttcgact gggcatatct cggattttat
     9901 ggtattatag gatttaaatt tttaatacag gggcttatag aaatactaag gaaagcgtat
     9961 gttgtcatca ataatgaaaa gatagccctc aagccagaca caacaacaaa gggaaaaact
    10021 atattttgga aagacatcct taccataaaa agggttggta agaactatga aattttgtgt
    10081 tctgacaaga catcctatac catatatctg tcacattaca gctataaagt ggctgatgaa
    10141 ataaaaatag ccctcgaaac aatcgctgct gagaaagcta tactgattga aacactaaca
    10201 aattaataag ggacaagcgt tttacctgtc ccttattaat ttatgtattc tgtgtcaatg
    10261 catagttgtt tacccggcac tttcaatatc aatattcatc ccagcttttt atcttaatat
    10321 cctctttttt cagtgtacag aaggctctaa taaaagctga agccagcctt gcgtttgtta
    10381 ccagcggtat attaaagtca attgcgctgc ggcggataat ataaccattt gtcaactcac
    10441 gacgcgaaag gtctttgggt atattgataa tcatatcaaa cttcttttca gcaatcatct
    10501 ctttgatatt ataatctcct tcttcgtccg gccaattcac tgcaattgat tttatatcgt
    10561 tatcagcgag gaatttctga gttcccttcg tcgcatagag ttcataccct ttctcatcca
    10621 acattttgca ggcatccagc agatcaactt tcgatttcgt tgttccggat gaaatcatga
    10681 tgcgtttttg aggaatacgg taacccacag ataataacga cttcaatata gcttctgaga
    10741 agctgtcacc aatgcagcct acttcgccgg tcgaagtcat atcgacactc aacaccggat
    10801 cggcatgctg taagcgggtg aaagaaaact gtgaagcttt gatacctaca taatccagtt
    10861 caaaagctga tttctccggt ttctctaccg gaataccgag cattactttt gtagccagat
    10921 caatgaaatt gattttcaaa accttggaaa caaacggaaa actgcgtgat gaacgcagat
    10981 tacattctat caccttgatg taattatccc gggccaggaa ctggatatta aaaggtcctg
    11041 aaatgttcag agaagaagca atctcacggg caatattctt gatccgtcgg atggtctcga
    11101 tgtatatttt ctgcggagga aactgcgtag tcgcgtcacc agaatgcacc cctgcatatt
    11161 ccacatgttc ggaaatggca taaaccacaa tttctccctt gtccgcaaca gcgtcgattt
    11221 caatttcctt gcagcgttcg aggaactctg atattaccac cggatgtttt ttcgaaacct
    11281 cggtagccag atcaagaaag gctttcagtt gagtttcgtc gtaacaaaca ttcatggctg
    11341 cacccgatag cacgtacgat ggacgaacca gtacagggaa accaatatcc cttacaaatt
    11401 tattgacgtc gctgtaggtt gtaagctcct gccagcgcgg ctggtcaatt cttagttcgt
    11461 caagcatagc tgaaaattta tttctatcct ctgcacggtc aatatcctta gcctgtgttc
    11521 ccaatattcg catattggca gcagccagtt tcattgccag attgttcgga atctgacctc
    11581 cggtagaaac gatggtaccc attgggtttt cagcttccag gatatccaac acacgttcgt
    11641 aagtcagttc gtcgaaatag agccggtcgc acatatcgta atcggtcgaa acggtctcgg
    11701 gattgtaatt gatcatcacc gaacgaagtc cctgctgacg gatggtgttc aaagcattaa
    11761 cgccacacca gtcaaattcc acggaagagc caatacgata cgcccccgag cccaatacca
    11821 caaccgaacg tttatcgcct aaataatgta catcggtagc attaccatta tatgtcacat
    11881 agaggtaatt ggtcaacgcg ggatattctg cagctagggt atcaatttgt tttacaacgg
    11941 gaacaatacc cctgcttttg cggtattccc gtgttttttt ggtttcatcg tccatatcgt
    12001 ggcttttact tttcaccaca atacgggtaa tctggaaatc ggagaaacct tttttcttag
    12061 cttcaagcaa aaactcgtct ggtaagtcac tcaacttatg atatgaggct agttcttttt
    12121 ccaaatctac aatgtgttgc agtttgtata aaaaccattt gtcgattttg gtcagttcgt
    12181 gaattttatc cacattgtaa ccttcgtgga atgcctgcga aaggaagaaa atccggcggt
    12241 ctgttggtac agatagatct ttctccacat ttccggtttc gaattcctta tttccaacaa
    12301 aaccgtgcat tcccaaacct accattcgaa gtcctttctg gagaacttcc tcaaaattcc
    12361 gtccgattga catgatttca cccactgatt tcatgctgct acccaattcg cgtgatactc
    12421 cgtgaaactt acccaaatcc cagcgtggta ttttgcagac tacatagtcg agcgaaggct
    12481 caaagaaagc tgaagtaact ttagtgattg agttcttcaa ttcagtcagt ccgtatccga
    12541 gtcccagttt ggcagcgacg aatgccagcg gataacctgt agccttcgaa gccagtgccg
    12601 atgaacggct cagacgcgca ttcacctcaa taacgcggta atcttccgaa acgggatcga
    12661 aagcatattg cacgttacat tcgcccacaa taccgatgtg gcgaatgata cgaatggaaa
    12721 gttcgcgtaa cttgtagtat tcgctgtttg tgagggtttg tgatggtgcc acaacgatac
    12781 tttcaccagt atgtatgcca agtggatcga agttttccat gttacatact gttatacagt
    12841 tgtcgtaacg gtcgcgtacc acttcatatt caatttcctt ccagcctttc agcgattttt
    12901 cgaccagcac ctgtggcgaa taggagaagg ctttatcaac cagtgttttg agttcggtat
    12961 cgttatcgca gaaaccactg ccaagtccgc cgagcgcata agctgcccgg acaattaccg
    13021 gataccccag gctgttggca gcgcgcagtg catcatcgag cgatgtaaca gctgcacttt
    13081 tgatatattt aactcctatc tcggagagtt ttcggttaaa aatctctcta tcctctgtat
    13141 ctataatcga ttgtacagga gttcccagca cctttacatt atatttttca agaattttat
    13201 tgcgataaag tgcaacacca caattgagcg ctgtctgccc tccgaaagag agaaaaatac
    13261 catcaggatg ttctttttcg ataacctgtt caacaaagtc gggggttaca ggcagaaaat
    13321 ataccttatc ggcaatacct tcggatgttt gcactgtggc gatattcgga tttatcaata
    13381 cagtttgcac tccttcttcg cgcagtgctt tcagagcctg tgaaccggaa tagtcaaatt
    13441 caccggcttc tccgattttc agagcgcccg aaccaagaac cagtactttt ttaatctctc
    13501 ccttttcggc cgatttgtaa ttatgcttta caaccaattt cgcgtccagc tcttcttcta
    13561 tcagtttggg cacggaaata ttattttcat ttatcgatct gataaataca tcgaaaagaa
    13621 attccgtatc agtagggcca gctgatgctt cgggatgaaa ctgcgccgag aaccagggtt
    13681 tggttttgtg acgaatacct tcgtttgttc cgtcgttcat attgatgaaa agcggttccc
    13741 agtcggctgt cagtgtattg ctgtcaacag caaatccatg attttgcgag gttacaaatg
    13801 cccgatgagt tccagccagt tgaactggtt gattacagct acggtgaccg tatttcagtt
    13861 tataagtttt ggcaccgcct gccatagata acaactgatt tcccatacag ataccaaaaa
    13921 tcggtttatc agttaacatg gccttgcgga tattggcaac tgttgcgtca caataagcag
    13981 gattaccagg tccgttggaa ataaacaacc catcgaaatc gagcccattg aaatcataat
    14041 cccaaggcac ccttactaca gtagtatcgc gtttgagcag acatcggata atattgtgtt
    14101 ttactccaca gtccaccagc accactttat tttttccatt tccataagtg atgacttctt
    14161 tacaactagc ttttgccacc tgattttcgt catcgggatt aacaaaatca atatcttcct
    14221 tatcggggaa aaccagcttg ccgagcatcg tccccttctc gcgtacaagt tttgtaagtt
    14281 cacgggtatc tataccgtac acggcaggca cctgattttc tttcaaccaa tcgctaaggc
    14341 ttttggccgc attccagtgg ctgtattcaa acgaaaaatc ggaaacaata agaccagctg
    14401 cctgtatttt ttccgattcg taaaaggttg aaatgccgtt aataatttca tccttgggga
    14461 caccgtaatt tccaatcagc ggataagtga gcgtcagtat ctgcccggca taagagggat
    14521 ccgataaact ttcggtgtag ccgaccatcc cggtactgaa aacaacttcg ccggcaaccg
    14581 gcttttcata accaaatgat tttcctttga atactgttcc gtcttccaga acaagatgca
    14641 ggggtttgtg atgcatggtg agtatgttga atgtttgaaa ttatatgtca aaaaaatggc
    14701 aggatatgta cttttttgag aaattgcata cttatccaaa aagtaaacat tattctttat
    14761 tttccaccga attttgtgca aaagtacgga ttttctatca tttttacaac cggtagaaac
    14821 ccgaataaac ataaaaaaag tctttcggga taaaaaattg tgataaagta cgattgtata
    14881 gtaaattttg tatctttact gcgataatat cccttattaa gtcaacataa agcatagtcc
    14941 ggttatgaaa tcatttaaat ttgtttttat agttattttt acgttttcag ttctttcagt
    15001 gtttgctcaa attgagacat cggctccgat actctccgaa aaatttttag ttgaatcata
    15061 tgaaaaaaaa ctgaatacga agaatgttat cagttatgaa gacataaaag gaacacccta
    15121 ctggaaaaaa gaatatattg agggtaaact ttatttgaat gataccgttg tggttcaggc
    15181 acctcttcgc tacaatattt atacggatga aatcgaatac aagtcgaatg acagaaatta
    15241 cgctattggc aatcctgcat atctaaagaa agctttaatc ggtaaaacca cttttttgta
    15301 tatcccgttt attggtaatg gcggatatta cgaactcctc gaatcgggaa aatgctatct
    15361 gctgcaacga cggaccatgc gatttaagcc cgtggaatac gccaaaccca ttatcggcac
    15421 tgtacctgca gaatttatcc ctgaacccga cgtgttttat ctggtagtgg acaataaccg
    15481 ctggtacaaa atagtcagtc tcaaatcggt taaaaatgta atgctcgatc aggaaaaaat
    15541 gatcgacgaa ttcataagcc gggaaaaaat caggtcgatc aaaaaagaaa atttaataaa
    15601 aatcgttaag cattacaaca gctttgtaaa taattaattt ctcaacacgt ctcgcttccg
    15661 tttatttatc aatagtgact ttattgattt gttcattttt taaaatacga attaaatgct
    15721 ggtaattttt atgattagtg cctactttcc agtattatta taaaaaatca cacatcaatt
    15781 cgcatattaa gtaaaaatca gaaaaaaaat tat
//
LOCUS       DAOPYB010000056        15730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4007_length_15730_cov_8.394386, whole genome shotgun sequence.
ACCESSION   DAOPYB010000056 DAOPYB010000000
VERSION     DAOPYB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15730
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4007_length_15730_cov_8.394386"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1050
                     /locus_tag="PKH58_06035"
     CDS             163..1050
                     /locus_tag="PKH58_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX88620.1"
                     /translation="MFYLQSHIGELAALFVSVCWTMSALFFEKASKKIGSLPVNIIRL
                     ALAIVFLGLTTLVTRGRFFPSDASNYNWIWLGLSGIVGFFLGDLCLFKSYTVIGSRTA
                     ALIMSLAPMLTAIIGWIFLNEILEVMSILAIVLSITGIMIAIANKNLKLNIPLKGFLL
                     AFGGALGQAVGLILSKKGMGDYDPVAATQIRAIFGLICFVVFITFVGRWDKVVSATKD
                     KVSMKSVIVGSVFGPFIGVALGLFAIQNTQTGIASTLMALVPIFIIVPSSIMFKEKIT
                     ARQIVGAVISIVGASLFFL"
     gene            complement(1060..1944)
                     /gene="dapA"
                     /locus_tag="PKH58_06040"
     CDS             complement(1060..1944)
                     /gene="dapA"
                     /locus_tag="PKH58_06040"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNX88621.1"
                     /translation="MLNTKLIGMGVALITPFKEDETIDFEALKRLVEHQIKNGTDYLV
                     VCGTTAETPTLTDDEKDEITRFVVNCVAGRIPIVLGVGGNNTKLVVNQLQTKDFSGID
                     AILSVTPYYNKPSQEGIYQHYAAIARNSPLPVILYNVPGRTGVNMLPETTIRLANEFP
                     NICAVKEASGNFTQIDDIIKNKPADFMVISGDDGITFPLITLGAVGVISVIGNAFPKE
                     FSKMVRLALQGDYNNARQIHYKFTELFNLLFVEGNPAGVKSMLAVMGLIENKLRLPLV
                     PNTIKTYEKIRKVLNELI"
     gene            complement(2038..2649)
                     /locus_tag="PKH58_06045"
     CDS             complement(2038..2649)
                     /locus_tag="PKH58_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88622.1"
                     /translation="MKKLNQFLFKRRSAAHFAATDRNRRFVNYENANTVLLLFESDYI
                     EKNRYIRKVIEQLAADGKKVTAWGYLYNKKATTANLPSFKIMDKSTVDWFECPKEQLL
                     KELREETYDLLIDLTLTDHLPLQYICLYANAAFKTGMSRNIDNILDFKINIPLPENTE
                     TVSETTEDNKSDFEDLNESMFHTDQQFLYELIIFYLKNIQTTD"
     gene            complement(2745..4745)
                     /gene="ligA"
                     /locus_tag="PKH58_06050"
     CDS             complement(2745..4745)
                     /gene="ligA"
                     /locus_tag="PKH58_06050"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNX88623.1"
                     /translation="MEISEKIQTLRKELDKHNYNYYVLSAPIISDFEFDRLLKELQEL
                     EEAHPEYFDPNSPTQRVGSDRNTSFKQVKHTYPMLSLANTYSEGEVRDFCERVRKGLN
                     DDFELVCELKYDGTSISLTYENGLLKQAVTRGDGDKGDDVTANVKTIRSIPLRLHGKD
                     FPQLFEIRGEILMPWSVFDDLNREREEQEEVLFANPRNAASGTLKLQNPSEVAKRRLD
                     GYFYYMLGSNLPSDLHYDNLQAARKWGFKVSDAVRVCRTPEEVFNFINYWDVERKNLP
                     VATDGIVIKVNSLTQQRNLGFTAKSPRWAIAYKFQAEKAVTRLNSVSYQVGRTGAVTP
                     VANLDPVQLSGTVVKRASLHNAEIIRSLDLHIGDMVYVEKGGEIIPKITAVDKDARFL
                     IGDKVEFIKKCPECGTELLRIEGEAAHYCPNENACPPQIKGKMEHFVSRKAMNIDGLG
                     SETINLLYQNGLLHNIADIYELRIPDLSRLERLGMKSAYNIKDGVEKSKQVPFERVVF
                     ALGIRFVGETIAKKLALAFKTIENMEKARLDELVEVEEIGERIAQSVVNYFADERNID
                     VVERLKSYGLQMSVTEDKLENKSNILSGMTIVISGTFSKHSRDEYKTIIEQNGGKNTG
                     SVSAKTSFILAGENMGPEKFNKAEKLNVRIMNEDEFLELIGS"
     gene            complement(4782..6026)
                     /locus_tag="PKH58_06055"
     CDS             complement(4782..6026)
                     /locus_tag="PKH58_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX88624.1"
                     /translation="MNFEYFIARRIHFEKKRKKNVSRPAVRIATIAIAVGLAVMIVAV
                     GVVIGFKKEIRNKTIGFGSHIQISNFDTNNTYEMQPVKMNDTLLADLAHIPHVKHLQR
                     FVTKPGIIKTETAFQGIILKGVGKDYDWDFFKSNMLEGRIPVVNENTIHNEAIISKTI
                     ADLLGLKTGDSFYTYFLQDQMRVRKLKITGIYSTNFEDFDKIFMLTDIRIAQKLNDWE
                     EEKFGGLEILIDDFNKLEEVNNNVYDVCANQFDENGNGYYIQTIKQMNPQVFKWLDLL
                     DINVWVILFLMLAVAGFNMISGLLILILEKTNMIGIIKSYGATNWSVRKIFLYQSLFL
                     IGKAMIWGNIVGLGICALQYYFHIIPLDPVAYYVSSVPVTFNWLYILLLNIGTLVASV
                     LMLVGPSYLITKISPATIIRYE"
     gene            complement(6187..7203)
                     /locus_tag="PKH58_06060"
     CDS             complement(6187..7203)
                     /locus_tag="PKH58_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNX88625.1"
                     /translation="MTSRKPVASPDKNSRLHLNSRILVFGVIGISIIHTIYMNVMGYS
                     PLNHNSCVVFKSLPKWFFQLYESFIELFVVVILGVFVGVLIERYFNKIKRFYPKNQWL
                     AFLYGAILPVCSCGVLPLIDSMKKRTSLKTIITFIIAAPLLNPYIMVVSFTVLGWKYM
                     VLRIVFSFLLSFLAGWLLEFVSNRYKTFSWGEYQACVSDCDFIPSRDPFIKTTLMTKK
                     LVPYILAAGTLSFIFAIINPKQFLEALNFSLEPWSTIVMTAIGIPLYVCNGTDIIFLK
                     PLMQYTDLSMGSAIAFSLSASAVCVSSIAMLLKFIGKNLTIALVSILIVLIFIFSAVI
                     NLFL"
     gene            complement(7252..7617)
                     /locus_tag="PKH58_06065"
     CDS             complement(7252..7617)
                     /locus_tag="PKH58_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88626.1"
                     /translation="MKRISSILFFLAVLCIQLTGQIRLEYTTPRLQTYKPGDKIELNI
                     NVKTRPETCTKGMKQVKIYMSGLKKEKLSDWKELSKGLWTTHITLSVLETAKKSGKLT
                     IIRRVDKEDLFKQELFPVE"
     gene            complement(7621..7959)
                     /locus_tag="PKH58_06070"
     CDS             complement(7621..7959)
                     /locus_tag="PKH58_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX88627.1"
                     /translation="MKTKIIKISFFSILLFAIVFNACEKLSQYYKIDTDKCTSCMKCV
                     AVCGYGAIQVDSIYRTTSEGFEYETTVIIDPKKCVGCGECYNKCSYDAIALYVKDKYV
                     DGNSGASRVK"
     gene            complement(7971..10652)
                     /locus_tag="PKH58_06075"
     CDS             complement(7971..10652)
                     /locus_tag="PKH58_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX88628.1"
                     /translation="MLPFTAHAEVSQQAADSIAHKLNLAEDCSTCSYYATEHSVAGMI
                     NTIIIAVLVLLGSIWLYAEKKKFYILLLGVLISGAVAASYFAAPYFFSQQENVPENCP
                     VNENKGSENTFTSPGSEFEPVETVKADKDTTAIVAVKSDEYKATPSDEFKSASSEEFS
                     NGSKDEFTSVSTDEFSSDTSEFSTENAKTDPTKTEEIRKINYTMIYEPLAIFLILALI
                     SLLIKYEWFRKIRGFFLLASVIYLGFYRGACPCMISSFQNGVLMIFGVPVAWESLLWF
                     LVLIPATYLFGKVWCGWLCHLGALQELIGRSSKLDFLTGRKVQRTLKIIQISVFVIWI
                     LQMLITRTNIFCEYDPFKVAFNLFSANWLGWILLPVLIVSSLLIHRPFCRTVCPVGLI
                     LGWVSLIPGAKKLDKTTSCINCKMCSNECAVRAMIHENKTTTLKQEDCIMCGECMSSC
                     KKQDALHVKRIFRKKNVISVVLIMFFAVQGTYAQWECPSRMGATLKPIGGSNLMWTSE
                     ITTSGGFIGDYKIANLMLFGGLDYSINHHTFYVEGGVKNWVRNDSLNITNSQALKFGM
                     REAFYRFSNDNHKLTIGLQSTKSDDYFLINERLAGANYKATFGRFNLNVLGGSVLKQS
                     SRNGTFCTVGYLINVVPGRERALIGNKFGQTNVSMVSLTYVPSSGRDEFSSEFSTSDA
                     SKSIFKLNEAGGLAYYEFGNGKTISPFITGIYASSSLAGINLKPELLYQAAENNHALL
                     YNIEVSKNINWSGGQLTKLMARYYGLHEISDGAVALNSYSNVFAGDVLRLDALELPFF
                     QAGIKHSFPTIKSSVKLQAALQTGKTSGFIDDNFSTVPARMKEFDLSFSKNFGKYILI
                     TAYGGYIEHPTLSVDGSEFRYLKDASPWGKVEVRFTF"
     gene            complement(10761..11126)
                     /locus_tag="PKH58_06080"
     CDS             complement(10761..11126)
                     /locus_tag="PKH58_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88629.1"
                     /translation="MNKLILSAFFLFFSISVVSACNFEFTTKDNKTSVKPGDEFVINV
                     KLSLIHRNCKVAAKDTKFKFEGMDIVGATDWKQESPMIFTRQIKAKANKDNSKKIMLT
                     ATRSCEKDGGYGVFTLPKN"
     gene            11325..12074
                     /locus_tag="PKH58_06085"
     CDS             11325..12074
                     /locus_tag="PKH58_06085"
                     /inference="COORDINATES: protein motif:HMM:NF021540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HNX88630.1"
                     /translation="MKKIFLSAFILLIFCNLTSGQTNGQLKVVFTTSTYYGDYKPKHI
                     LAVWVSKADGTFVKTLMAYTSATKYRQYLTKWKAATSSTYNMVDAVTGATLNTHGSLT
                     CNWDCTDVSKAVQNDGEYRVNIEFTESNAAGKIATISFTKNSTPASTFTNITTSTNIT
                     QINVVWSPVNTAVDNVDDDTYYKIYPNPVNSLLFVSGSDIRQVSLYDMQGKNILNTTE
                     NQLDFSDYKPGTYLIKIRTDKGIVMRTALKK"
     gene            13079..13492
                     /locus_tag="PKH58_06090"
     CDS             13079..13492
                     /locus_tag="PKH58_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88631.1"
                     /translation="MKKVLLLPFLIGFIFTSCEPVPEDLTTKVIYNFSINSKEWQVHT
                     DAAGLNRYYSCSLPVSNLNSNVYDDGAVIAYIVMDGYQQVLPYIRHYENSNGERWTRT
                     VDFDFSDGAVNFYITNSDFAVDPPGNMDFRVVLMW"
     gene            13582..14130
                     /locus_tag="PKH58_06095"
     CDS             13582..14130
                     /locus_tag="PKH58_06095"
                     /inference="COORDINATES: protein motif:HMM:NF040334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6144 family protein"
                     /protein_id="HNX88632.1"
                     /translation="MFDIRKIQEQVVFGAVKEASDDETARKIVYGDSTTSVVEDDPAW
                     VKNTMHRLENRFDPQSVKNIRMNCQCGYGMDEKLSLVRELMQSARNMDEFANQEKARA
                     AGLSYHDGELFLQFPFCPCPILAGVDKLDTSAWCQCTNGYSKVLFEKAFGCEVSVELI
                     KSIKTGDEICLMKIIPHSEIWR"
     gene            complement(14378..>15730)
                     /locus_tag="PKH58_06100"
     CDS             complement(14378..>15730)
                     /locus_tag="PKH58_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNX88633.1"
                     /translation="SITSLFLFIVMFFASTLSYAQELVTNGNFEAGQTGWIGNAFNVV
                     TEGGNSYNAANVAAAGEAWAVNLSQVLSLTSGTTYQLKFDAWSDQSRTLIAGIGLNEG
                     PWSASTKTITLGLTSQTFTLTLVAPATSANSRVLFDMGAAAGFVGIDNVSLQVVTPTC
                     SDGVKNGDETGIDCGGSCAACAVSGPTTAAPTPPTRTATDVVSIFSDAYTNISVNAWG
                     PDWGPSSSRFSDATIASNATKVMNVAAGQIFAGIDFAPSLFDATSFTYFHLDYWIANP
                     LPVGQVVNIKLSNHLGGSGETSAIQYTVSAPQAGSWAQLDIPLADFVAASDPANLNRS
                     AIAQIVISAARADGNVPVDIYLDNIYFYKNASAGVNNQKANNLSIYPNPVVNKLTVTA
                     PLEISELTVRNVLGQSIKSVVVNGVSQTIDIDDLATGNYFVVAKMKNGQLSTQKFSKL
                     "
BASE COUNT         4604 a         3155 c         3120 g         4851 t
ORIGIN      
        1 attagccttc caagctgagg gtcgcgggtt tgagccccgt cttccgctct caaaaatgaa
       61 ggccctgaca attactgttg gggtcttttt tttattttaa tatccactaa tagagtggta
      121 tagctatcaa attacaaatt caaacataat tcattctgta gtatgtttta tctgcaatca
      181 catatcggcg aacttgccgc tctttttgta tcggtatgct ggacaatgag cgctcttttc
      241 ttcgaaaaag cttctaaaaa aattggttcg ttgccggtga atattatcag actggctctg
      301 gctatcgttt ttctgggact taccacacta gttacacgcg gccgattctt cccctccgat
      361 gcaagtaact ataactggat ttggctcgga ctatccggaa ttgtgggttt ttttctgggt
      421 gacctgtgtc tgtttaaatc ctatactgta atagggtcac gtactgctgc attgatcatg
      481 agtcttgcac ctatgctcac agctatcatc ggctggatct ttctgaatga aatccttgaa
      541 gttatgagca tattggctat tgtgcttagt attacaggta ttatgattgc aattgccaat
      601 aaaaatctga aactgaatat acctctgaaa ggatttctgc ttgcttttgg tggtgcgctc
      661 gggcaggctg taggtttaat tttaagtaag aaagggatgg gcgattacga tcctgtggct
      721 gcaacacaaa tccgggcgat cttcggtttg atatgttttg ttgtgtttat tacttttgtg
      781 gggagatggg ataaggtcgt ttcagctaca aaagataaag taagtatgaa atcggtgatt
      841 gttggctcag tattcggacc atttatcggg gtagccttgg gcttatttgc cattcagaat
      901 acgcagacag gtattgcatc aacactgatg gcacttgtac caatctttat aattgttcca
      961 tcgtccatta tgtttaaaga aaaaattaca gctcgccaga ttgtgggagc tgtaatcagt
     1021 attgttggag caagtctttt ctttttataa attctgattt tatatcagtt cgttcagcac
     1081 ttttcgtatt ttttcgtaag ttttgatggt gtttggtact agtggcagtc gcagcttgtt
     1141 ttcaataagt cccatcacag ccagcatact tttcactccc gcgggattac cttcgacaaa
     1201 gagtaaatta aataattcgg taaacttgta atgtatctga cgggcattgt tataatctcc
     1261 ctgcagtgca agtcgtacca ttttactgaa ttctttcgga aaagcattgc caataacaga
     1321 aataacacct acagctccga gtgtaatgag tgggaaagta atgccatcgt cacccgatat
     1381 gaccataaaa tcggctggtt tgtttttaat aatatcatct atctgagtaa aattacccga
     1441 agcttctttt acagcacaaa tattcgggaa ttcattcgct aaacgaattg ttgtttccgg
     1501 aagcatgttt acgcctgttc gaccgggaac attgtaaagt attaccggta ggggagaatt
     1561 ccgggcaatg gctgcatagt gttgatatat gccttcctgc gatggtttgt tgtaataggg
     1621 agtaacggac agtatggcat caattccgga aaaatctttc gtttgcaact ggttgacaac
     1681 cagttttgta ttatttccac caacacccaa tacaatcgga atacgtccgg caacacagtt
     1741 cacaacaaag cgtgtaattt catctttttc atcatcggta agtgtgggag tttctgcagt
     1801 ggtaccgcaa actacgaggt agtcagtccc atttttaatc tgatgctcaa ccaggcgctt
     1861 gagagcttca aaatctattg tttcatcctc tttgaagggt gttatcaggg caacacccat
     1921 tccaatcagt ttagtgttca gcatttggaa agtatctcgt taaatcatgc agcagtgtaa
     1981 attggaactc atatctgcag gatgattgaa attcaaattt tttgcaaaaa tacggtttta
     2041 atctgtagtt tgtatatttt tcaggtaaaa aattatcaat tcgtacagaa attgctggtc
     2101 tgtgtgaaac atactttcgt tcagatcttc aaaatcggat ttattatctt cggtagtttc
     2161 agagaccgtt tccgtatttt cgggtaaggg tatattaatt ttaaaatcca gaatgttgtc
     2221 aatattccgg ctcattcctg ttttgaatgc agcattggca tacagacaaa tgtactgtaa
     2281 tggtaaatgg tcggtaagtg taaggtcaat cagcagatcg taagtttcct cgcgaagttc
     2341 tttcagaagt tgctctttgg gacattcgaa ccagtcaact gtacttttat ccatgatttt
     2401 gaaactgggc aggttagcag tggtagcctt cttattgtat aaatatcccc aggcagtaac
     2461 ttttttacca tcggcggcaa gctgctcaat gactttccgg atatacctgt tcttttcaat
     2521 ataatcgctt tcaaacaaca acagcacagt gttggcattc tcgtagttga caaaacgccg
     2581 gtttcggtca gttgccgcga aatgagccgc cgatcgtctt ttgaaaagaa attgatttaa
     2641 ttttttcatg tttccggtgt ttctgaaacg tcacgttcag ttttgtttac aaatttatgt
     2701 aaaaatgttg aattaatgta actgttgcgt tcattttctt caacttaact gccaatcagc
     2761 tccagaaatt catcttcatt cataattcgg acattgagtt tctctgcttt gttgaatttt
     2821 tcgggtccca tattttcgcc tgccagaatg aaacttgttt tagctgatac acttccggta
     2881 tttttccccc cgttttgttc tatgatggtt ttatattcat ccctcgaatg ctttgagaaa
     2941 gtaccgctaa tcacaattgt cattccggaa agaatattgc tcttattctc cagtttatct
     3001 tctgtaaccg acatttgcag tccgtatgat ttaagccgct caacaacatc aatattacgt
     3061 tcgtcggcaa agtagtttac aacgctttga gcaatccgtt cgccgatttc ttccacttcc
     3121 acaagttcat caagccttgc tttttccata ttttcgattg ttttaaatgc cagtgccagt
     3181 tttttcgcga ttgtttcgcc cacaaaccga attccgagtg caaatactac gcgctcgaaa
     3241 ggtacttgtt tcgacttctc cacaccatct ttaatgttgt atgccgattt cattcccagt
     3301 cgttcgaggc gtgataagtc gggaattctg agttcataaa tatcggcaat gttatgcagc
     3361 aatccattct gatacagtaa gttgattgtt tcactgccta gtccgtcaat attcatggct
     3421 ttacgactga caaaatgctc catttttcct tttatttgtg gcggacatgc attttcattg
     3481 gggcagtagt gggcagcttc gccttcaatc cgtagcagct ctgtaccaca ttcgggacat
     3541 tttttgataa attccacttt atcgccaatc agaaaccggg catccttatc gactgctgta
     3601 attttcggaa taatttcacc tcctttttct acgtacacca tatcacctat gtgtaggtcg
     3661 agcgaacgga taatctcggc attgtgcagg gaagcacgtt ttacgactgt acctgacagt
     3721 tgaaccggat caagatttgc tacgggagtg acagctccgg tacgtcctac ctgatacgac
     3781 actgaattga ggcgagtgac tgctttctct gcctgaaatt tgtaggcaat agcccagcgg
     3841 ggtgatttcg ctgtgaaacc caagttgcgc tgctgtgtca gcgagttgac tttaatgaca
     3901 atgccatcgg ttgcaaccgg aagatttttc cgttccacat cccaataatt gatgaaattg
     3961 aaaacttctt ctggtgtacg gcatactctc acggcatcag aaactttgaa accccatttt
     4021 cgggctgcct gcaggttgtc gtagtgtaaa tcggacggaa gattactgcc taacatgtag
     4081 taaaaataac catcaagacg tcttttagcc acttcggatg gattttgcag tttgagggta
     4141 cccgaagctg cattacgcgg attggcaaat aaaacttctt cctgttcttc gcgttcgcgg
     4201 ttgagatcat caaatactga ccacggcata agtatttcgc cccggatttc gaacaactgc
     4261 ggaaaatcct ttccgtgtaa acgaagcgga atactccgga ttgttttcac gttggctgtc
     4321 acatcatccc ctttatctcc atcgccacgt gttacagcct gtttcagtaa cccgttttcg
     4381 taagtcaatg agatggaagt accatcgtat ttcagctcgc agacaagctc aaaatcatca
     4441 ttcagaccct tgcgtacacg ttcacagaag tcccgtactt cgccttcgga atacgtgttg
     4501 gccagtgaga gcatcgggta tgtgtgtttt acctgtttga aggaagtatt tctatcactg
     4561 cctacacgtt gagtggggga gttggggtcg aagtattcag gatgagcttc ttccagttct
     4621 tgcagctctt tcaacaacct gtcaaattca aaatctgaaa taataggtgc tgacagcaca
     4681 taataattgt agttgtgctt atcgagttcc ttgcgcagtg tctgtatttt ttctgatatt
     4741 tccataataa gtcaaaatca aagttcaata actcaataaa gctactcgta acgaatgata
     4801 gttgccggcg aaattttagt aattagataa gatggaccta ccagcatcag caccgacgcc
     4861 acaagtgtgc cgatattgag aagcaaaata tacagccagt tgaatgtgac aggtacactg
     4921 ctcacatagt aagccactgg gtcgagcgga atgatgtgga aataatactg cagggcacat
     4981 attcccagtc ccacgatatt tccccaaatc atggctttac cgataaggaa cagtgactga
     5041 tacaaaaaaa tctttcgtac tgaccagttg gttgcaccat acgatttgat aataccgatc
     5101 atattcgttt tttcgagtat aaggatcaac agtcccgata tcatattaaa tcctgccaca
     5161 gcaagcatca gaaagaggat aacccataca tttatatcga gcagatcaag ccatttgaat
     5221 acctgcggat tcatttgttt gatggtctgt atataataac cgttgccatt ttcgtcgaac
     5281 tgattggcac agacatcgta tacattgttg ttgacctctt ccagtttgtt gaaatcatca
     5341 atgagtattt ccagcccgcc gaatttttct tcctcccagt cattaagttt ttgagcaata
     5401 cggatgtcgg tgagcataaa aattttatca aagtcttcga aattggtgga atatattccg
     5461 gtaattttca actttcgtac cctcatctga tcctgcagaa agtaagtata aaagctgtca
     5521 ccggttttca gtcccagtaa atcagctatt gttttcgaaa tgatggcttc attgtgaatt
     5581 gtgttttcat tcacaacagg aatcctgccc tcaagcatgt tggatttgaa aaaatcccag
     5641 tcatagtctt tgcccacacc tttcagaatg ataccctgaa atgcggtttc agttttgatg
     5701 attccgggtt ttgtaacaaa tcgctgaagg tgttttacat gcggaatatg agccagatca
     5761 gcaagcagcg tatcattcat tttgacaggc tgcatttcgt atgtattatt ggtatcgaag
     5821 ttgctgattt gtatatgtga gccgaatcca atggttttat tacggatttc ttttttgaaa
     5881 ccaatgacca cacctacagc cacaatcatc acggcaagac ctacagcaat tgcaatggta
     5941 gcaatgcgta ccgcaggccg ggatacgttt ttcttccttt ttttctcgaa atgaatacgc
     6001 ctggctataa aatattcaaa attcatgaat tcaaattgtt tgcaccggta tcaccatcaa
     6061 cattcgacaa aacatccgga ttgttatttt tacttacaaa aatacagaaa aagtatgtat
     6121 aataaaaata gaatctgtcc tgcttaggaa gaaagaaagt aagtcgccgt ttttacatcc
     6181 cggatactaa agaaacaaat tgattacggc agaaaatata aaaatcagta cgataaggat
     6241 tgaaacgagg gcaatagtca aattcttgcc gataaatttt aagagcattg ctattgaaga
     6301 tacacaaaca gccgaagccg atagtgaaaa agcaatggca cttcccatgc taagatcggt
     6361 atattgcatc agcggtttca gaaaaatgat atcggtgcca ttgcacacat ataaggggat
     6421 gccgattgcc gtcattacaa tcgttgacca gggttctaat gaaaaattga gcgcttccag
     6481 aaattgcttc ggattgatta tagcaaagat aaagctgagt gtacctgccg ccaaaatata
     6541 tggaacaagc tttttggtca tcagtgtggt tttaataaac ggatcgcggc tggggataaa
     6601 atcgcagtcc gaaacgcaag cctgatattc gccccatgaa aatgtcttat atctgttgga
     6661 tacaaattcg agcaaccaac cagccagaaa cgaaagtaaa aaagaaaaaa caattcgcag
     6721 taccatatat ttccaaccca aaaccgtaaa ggaaaccacc atgatatacg gatttagcaa
     6781 tggggctgca atgataaagg taatgatagt tttcagcgag gtgcgttttt tcatggagtc
     6841 gatcagcggt aaaacgccac aggagcatac aggcaatatg gcgccataaa gaaatgcaag
     6901 ccactgattt ttaggataaa atcgttttat tttattgaaa tatcgctcaa tgagcacacc
     6961 gacaaatacg cctaatatga ccaccacaaa caattcgatg aagctttcgt ataactgaaa
     7021 aaaccacttc ggtaatgatt taaacaccac acaactgttg tggttaagag ggctgtaacc
     7081 catcacattc atgtatatgg tatggataat cgaaatgcca attacaccaa aaacaagaat
     7141 acgggaattg agatgaagcc gggaattttt atcgggtgaa gcaacgggtt ttctgctggt
     7201 catgaaacga ttattagtaa taatgaatta aaaagtatgt aaagaatgat tttattctac
     7261 agggaataat tcctgtttaa ataaatcttc cttgtccacc ctgcgaatga tggttagttt
     7321 tccggatttt ttagcagttt ccagcaccga gagtgtaata tgtgtcgtcc agagaccttt
     7381 tgaaagttcc ttccagtcgg acaatttctc ttttttaagt ccgctcatat aaatctttac
     7441 ttgcttcatc ccttttgtgc aggtttccgg tcgtgttttg acgttgatgt ttaattctat
     7501 tttgtcaccg ggtttgtatg tctgtaagcg gggagtggtg tattcaagcc ggatctgacc
     7561 tgtcagttga atgcaaagta ctgccaaaaa gaaaagtatg gacgatattc gcttcatcgt
     7621 ttatttcact cttgaagctc ccgaattacc atccacatat ttatccttca cataaagtgc
     7681 aatggcatca tacgagcatt tattataaca ttcgccacaa ccgacacact ttttgggatc
     7741 aattatcaca gttgtttcat attcaaatcc ttccgaagta gtccggtaga tggaatcaac
     7801 ctgtatagca ccatatccgc aaactgccac acacttcatg caactggtac acttgtctgt
     7861 gtcaatttta tagtactggc tgagtttttc gcaggcattg aatacaatgg cgaaaagcag
     7921 aatggaaaaa aaagatattt taattatttt tgttttcata caactccatt ttaaaaggtg
     7981 aaacgtacct ccacttttcc ccagggcgaa gcatctttca ggtatctgaa ttcactacca
     8041 tccactgata acgttggatg ttcgatgtat cctccataag cagttatcaa aatgtatttt
     8101 ccgaaattct ttgaaaatga aagatcaaac tctttcatac gtgcaggaac ggtactgaaa
     8161 ttatcgtcaa tgaatccaga tgtttttcca gtttgaagcg cagcctgcaa tttcaccgac
     8221 gatttaatgg tcgggaatga atgtttaata cctgcctgaa aaaacgggag ttcaagggcg
     8281 tcgagtcgca acacatctcc tgcaaaaaca ttgctgtagg aattaagagc tacagctccg
     8341 tcagagattt catgcaaccc gtaatatcta gccatcagtt tggtaagttg tccgcccgac
     8401 cagttaatat ttttgcttac ctcgatattg tacaataggg catgattatt ctctgcagct
     8461 tgataaagca attccggttt aagattgata cccgccaaac ttgaacttgc gtatattccg
     8521 gtaataaaag ggctgattgt ttttccgtta ccgaattcat aataagccaa cccgccggct
     8581 tcattcagtt tgaaaatact tttcgaagcg tccgaagttg aaaattcaga actgaactca
     8641 tctcttccgg acgaaggtac ataggtcagc gacaccatcg atacgttggt ctgaccgaat
     8701 ttattgccga tgagagcacg ttcacgtcca ggaaccacat taatcaggta acccacagta
     8761 cagaaagttc cgttacggga tgattgtttc aacactgaac cacccaatac attcaggtta
     8821 aatctaccga atgttgcttt atagtttgct ccggccaagc gttcgtttat cagaaaataa
     8881 tcgtcacttt tggtggattg cagtccgatt gtcagcttgt gattgtcgtt cgaaaaacga
     8941 taaaaagctt ctctcatccc gaatttcagt gcttgcgagt tagtaatatt taggctgtcg
     9001 ttacgtaccc agtttttaac accgccttct acatagaagg tgtgatgatt gatgctgtaa
     9061 tcaagtccgc caaatagcat caggttggca attttgtaat caccgataaa tccgcccgag
     9121 gtggttattt cagaagtcca catcaggttg cttcctccga taggtttcag agttgcaccc
     9181 atacggctcg gacattccca ctgtgcataa gtgccctgca ctgcgaaaaa cataatcagc
     9241 acaacagata tcacgttttt cttacgaaaa attcttttta catgcagagc atcctgcttt
     9301 ttgcatgaag acatacattc gccacacata atacaatctt cttgtttaag ggttgttgtt
     9361 ttattttcgt gaatcatagc acgtactgca cattcattgc tgcacatttt acagttgata
     9421 cagctggtgg ttttatctag tttttttgca ccgggtatca gtgaaaccca acctaaaata
     9481 agtcccacag gacaaacggt acggcaaaaa gggcgatgga taagcagcga actgacaata
     9541 agtaccggca ggagtatcca gcccagccag ttggcagaga agaggttgaa ggcaaccttg
     9601 aatggatcat attcgcagaa tatatttgta cgggtgatga gcatctgcag tatccaaatt
     9661 acgaaaaccg aaatctggat aattttcagc gttcgttgta cttttctacc tgtcagaaag
     9721 tcgagttttg aactgcgccc gatgagttcc tgaagtgccc caagatgaca cagccagccg
     9781 caccacactt tgccgaatag ataggttgct ggtattaaaa ccagaaacca gagtaaactt
     9841 tcccaggcaa caggaactcc aaaaatcatc agaacgccgt tttgaaatga ggatatcata
     9901 cacggacaag ctccacggta aaatccgaga tagattaccg aagctaacaa aaagaaacca
     9961 cggatttttc gaaaccattc atattttatc agaagactta tcagcgccag tatcagaaag
    10021 atagcaagtg gctcataaat catcgtataa tttattttac gaatttcttc ggtttttgtc
    10081 ggatcagttt tagcattttc agtagaaaat tcacttgtat cagaactgaa ctcgtcagtt
    10141 gacacactgg taaattcatc ttttgatcca tttgagaatt cttctgaact cgcgcttttg
    10201 aattcatcgg aaggagttgc tttatattca tctgatttaa ctgcaactat agctgtcgta
    10261 tccttatctg cttttaccgt ttcaacgggc tcaaattcac tgccgggtga agtgaacgta
    10321 ttttcagatc ctttattctc atttaccgga caattttcag gaacattttc ctgttgtgaa
    10381 aaaaaatagg gtgcagcaaa ataactggcc gctacagctc ctgaaattag aactcccagc
    10441 agaagtatgt aaaatttctt tttttctgca tataaccaga tgcttcccag taataccaat
    10501 acagcaataa taatggtatt gatcataccg gcaacagaat gttcggtggc ataataactg
    10561 catgtgctgc agtcctcagc aaggttcaat ttatgtgcaa tgctgtcggc tgcctgctgc
    10621 gaaacttcgg catgtgctgt aaagggtagc ataactatca aaaacagcaa gagcagaatg
    10681 ataatctttt gtttttttat cacactaata aataatgatg taagtgtttg atatttagag
    10741 aatatacagg ataatacagc ttaattttta ggcaaagtga aaacgccata tccgccatcc
    10801 ttttcgcagg aacgggttgc tgtgagcatt attttcttgc tgttatcctt atttgccttt
    10861 gcttttatct gccgggtaaa gatcatgggt gattcttgtt tccagtcggt agcaccaaca
    10921 atatccattc cttcaaactt gaattttgtg tctttagcag caactttgca atttcggtgt
    10981 atcagtgaca gtttaacgtt aatcacaaat tcatctcccg gtttaactga tgttttattg
    11041 tccttggtgg taaactcaaa attacaggca cttacaacag agatgctgaa aaaaagaaaa
    11101 aacgctgaca aaattaattt attcataaaa gtgagttatg ttattaatgt gtaatgtata
    11161 tcttatttaa atttaaaaaa aattatttga ataacgatga ttattccatc ttattatatc
    11221 tttgtgatat taaaataata aattgcaaat ttacagtttt aattttaact ttcttattaa
    11281 gtacaaatat tttaagataa ttttcttcaa tccggtaaca atctatgaaa aaaatctttc
    11341 tttcggcttt tatattactt atattttgta atctgacttc tggtcaaaca aatgggcaac
    11401 tcaaggtcgt ttttactaca tcgacctatt atggcgatta taaaccaaaa catattcttg
    11461 ctgtatgggt gtcaaaagct gatggtacat ttgtgaaaac actgatggct tatacaagtg
    11521 caaccaaata ccggcaatac ctgactaaat ggaaagctgc gacttcttca acttataaca
    11581 tggtagatgc cgttacggga gcaacgttga acacacatgg ttctcttacc tgtaactggg
    11641 attgtacgga tgtttcaaaa gctgtacaaa atgacggaga ataccgtgta aacatagaat
    11701 ttaccgagtc caatgcagcc ggtaaaatag caaccatttc gttcaccaaa aactcaacac
    11761 ccgcttccac atttactaat attaccacat caacaaatat aactcagatt aatgttgtat
    11821 ggtcaccggt taatacagct gtagataatg ttgatgatga tacctattat aagatatatc
    11881 ccaatccggt aaattcattg ttgtttgtaa gtggaagcga catccgtcag gtgtcgttat
    11941 atgatatgca gggcaagaac atacttaata cgaccgaaaa tcaacttgat ttcagtgatt
    12001 acaaacccgg aacgtacctg attaaaatta gaacagataa aggcatagtc atgcggacag
    12061 cacttaaaaa ataatcaatt tattttattc aattaatatc tgataaccac agtaaaactg
    12121 aaaaaataat aatagagtaa acgtcaaaac aaacaacctg aaaatgttta ttttcactgt
    12181 tgtccgttaa aaaatgaggt tctttactat cactcagaat gacgacgttt tgtattgagg
    12241 ttgattgtta ggtggcaggt tagcggcttc atcgccagcc tgccacttaa taactgcttg
    12301 tcatccggaa cgaagtgaga gtactctggt tagttcattt ttagcaagtt tgcaggattc
    12361 atttttgtgt gtgctgtttg gacggttagc gacttagctt cgtgttccaa ttgacaaaat
    12421 tcacgtgatt tccgtttgtt cttttaaagt gatggatgcg aatccaagga aatccgtttg
    12481 tccttttata atgatagatg caattcacgc tattctcgtt cattctcatt gatttacata
    12541 aacaaatgac gcgattttca tgcgtcattt tattttgtta cattaacgaa tgggcgattt
    12601 ctgttgatta ttttttaatt ttaatgaaaa ttcccggaat tcaggttccc tgttttatga
    12661 ttaaagacat aattccggta aatagcagta tcaaatttct ttttccttta tctgagagag
    12721 agtaggtgct gatttttttt gcaaaggtag cctgacagga atggggattt gcaaaaaaaa
    12781 acaggcacaa gctgcttccc ggtgtgcggg tatatagcct gataatttta ttcgtcataa
    12841 tacaattgag ggtgccactc aaagtggcac tcccaataac aaagactgtt cagacattaa
    12901 attgatttgc aatcacatgt aatgttccat tttctgatca gaacagcaaa ttatttcaat
    12961 aaaagaatga aatgagatta tttacataaa ttttttcata tttttgtgtg ttataaggct
    13021 gaagcagcct gtatgaagca aaattataga tattgactta ctctgaaaaa cactacttat
    13081 gaagaaagtt ctcttgcttc ctttccttat cgggtttatt tttacatcgt gtgaaccggt
    13141 acccgaagat ctcaccacca aggtaattta taatttcagc atcaatagca aggaatggca
    13201 agtacatacc gatgcagccg gtcttaaccg gtattactcg tgcagcctgc ctgtgagtaa
    13261 tctgaatagt aacgtttacg acgacggagc agtgatagcc tatatcgtga tggatggata
    13321 ccagcaggtg ttgccatata ttcgtcatta cgaaaacagt aacggagaaa gatggacacg
    13381 caccgttgat tttgatttct ccgatggagc tgttaatttc tatatcacca attccgattt
    13441 tgccgttgat cctccgggga acatggattt tcgtgtagtt ctcatgtggt aagccaccaa
    13501 gcatttatca cacaggtgat ttttgtctga aaataattca gttcagcagg ttgtaataat
    13561 aaccaaccat tttaatttat catgtttgac attcgaaaaa ttcaggaaca ggtggttttc
    13621 ggagctgtga aagaggccag cgatgacgaa actgcacgaa aaatcgtgta cggtgacagt
    13681 accacatctg tcgtcgaaga tgatcccgcg tgggtaaaaa acaccatgca tcggctcgaa
    13741 aatcgtttcg acccacaatc ggtgaagaat atcagaatga actgtcagtg tggttacggc
    13801 atggacgaaa agctcagcct ggtcagggaa ctgatgcagt cggcccgtaa catggacgag
    13861 tttgccaatc aggaaaaggc acgggcagcc ggattgtcgt accacgatgg tgaactattc
    13921 ttgcagtttc cattttgtcc ctgtccgata ctcgcggggg tcgataaact cgatacatct
    13981 gcgtggtgtc agtgcaccaa cggatacagc aaggtgcttt tcgaaaaagc tttcggctgt
    14041 gaagtaagtg ttgagctgat taaaagtatc aaaacgggcg atgaaatttg cctgatgaaa
    14101 attattcctc acagtgaaat ctggagataa acccgtcttc cacatcctcc gtcagactca
    14161 ctcatgtatt attctgcttg tgattttata tttagcccaa taaggtttaa aaatgaatta
    14221 gtaaggagcg actatttatg ttgatactga tgcataaaag gatggcaaaa gcaacgaaaa
    14281 ttctggtttt catgtatgca gatgaattac ggaaattggt gaaaagaaaa aaagaaaagt
    14341 ccgcaatgaa aactattgcg gactttgaag aaaagattta taatttcgag aatttttgtg
    14401 tagaaagctg accatttttc attttggcta ccacaaagta atttccggtt gctaaatcat
    14461 ctatatcaat cgtttgtgaa actccattga caactaccga tttgatggat tgtcccaata
    14521 cattacggac agtcagttca cttatctcca gtggagcagt cacagtcagt ttatttacta
    14581 ccggattagg ataaatactc agattgtttg ccttttgatt atttacacct gcgcttgcgt
    14641 ttttataaaa atagatatta tccaaataaa tatccaccgg aacgtttcca tcggctctgg
    14701 cagccgaaat aactatttgt gcgatagcgc ttctgtttaa attagcaggg tcgcttgcag
    14761 caacaaaatc agctaaagga atatccaatt gagcccagct acctgcttgt ggagcactga
    14821 cagtgtattg gatagcactt gtttcaccag acccacccaa atggttcgat aattttatat
    14881 taactacctg acctacgggc aatggattgg caatccaata atctaaatgg aaatatgtga
    14941 aagaagtggc atcaaacaat gatggagcaa aatcaattcc tgcaaaaatt tgaccggctg
    15001 caacgttcat cactttagtg gcatttgagg caatggtcgc atcgctaaat ctacttgaag
    15061 atggtcccca atccggtccc caagcattta cggaaatatt ggtgtaggcg tcgctgaaaa
    15121 ttgaaacaac atctgttgca gttcttgtag gtggtgtagg cgcagcagta gtaggtccgg
    15181 aaacggcaca agccgcacaa gagccaccgc aatcgatgcc ggtttcatct ccatttttta
    15241 caccatcact acaagttggc gtaactacct gcagagaaac attatctatc cccacaaaac
    15301 cggctgctgc tcccatatca aaaagcactc ggctattggc tgaggttgca ggtgccacaa
    15361 gtgtaagagt gaaggtttgt gaagttaaac ccaatgtaat ggttttggta gatgcagacc
    15421 aaggtccttc atttagtcct atccctgcaa ttaatgttct actttgatcc gaccaggcat
    15481 caaatttcag ttgatacgtt gtacctgaag ttaagcttag cacctgactt aagtttacag
    15541 cccaagcttc tccggcagcg gcaacatttg cagcattata gctattgcca ccttcggtta
    15601 ctacattaaa tgcattgcct atccagcctg tttgtccggc ttcaaagttt ccgtttgtaa
    15661 ctaattcttg tgcataactt aatgtagagg caaagaacat tacgataaaa agaaataaac
    15721 tagtaattga
//
LOCUS       DAOPYB010000057        15559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4072_length_15559_cov_14.466976, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000057 DAOPYB010000000
VERSION     DAOPYB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15559
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4072_length_15559_cov_14.466976"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1313)
                     /locus_tag="PKH58_06105"
     CDS             complement(48..1313)
                     /locus_tag="PKH58_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNX88634.1"
                     /translation="MNEIICPNCKKAFKVDEAGFADILKQVRDRQFEEELKNRLSLAD
                     REKESAVKLAEANIRNSLQEQLARKDRELAELKAQSELALTEKLNKKEYEITQLKAKI
                     ENVEVEKKLAVTEAVQKLEKERDQLANDIKTKELERQNIESTLKQKFSTELQNKDAII
                     KYKDEEIARVKDMKLKLSTKMLGETLEQHCETEFNKLRATAFQKAYFEKDNDARSGSK
                     GDYIYRETDDVGNEIISIMFEMKNEGDETATKKKNEDFFRELDKDRNEKKCEYAVLVT
                     LLEADSEYYNTGIVDVSHRYNKMYVVRPQFFIPIITLLRNAAMNTIQYKAELALVRSQ
                     NIDITNFEEKMNKFKDGFARNYDLASRKFKDAIDGIDKTIRELQKTKDALLSSENNLR
                     LANEKTEDLTIKKLTYNNPTMKAKFDELK"
     gene            complement(2502..3557)
                     /gene="rfbB"
                     /locus_tag="PKH58_06110"
     CDS             complement(2502..3557)
                     /gene="rfbB"
                     /locus_tag="PKH58_06110"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNX88635.1"
                     /translation="MGKTILITGGAGFIGSHVVRLFVTKYPEYQIVNLDALTYAGNLE
                     NLKDVKDAPNYTFVKGDITDEKFIPELFARYSFDGVIHLAAESHVDRSITDPFAFIRT
                     NVFGTANLLNAAKNAWKGSMEGKRFYHISTDEVYGSLGSEGFFTEETAYDPRSPYSAA
                     KASSDHFVRAYYHTYGLPVVVSNCSNNYGSHHFPEKLIPLAINNIKKKLPVPIYGKGE
                     NVRDWLWVDDHARAIDVIFHQGVTGETYNIGGHNEWTNIDLIKELCAIMDRKLNRAPG
                     ESAQLITYVKDRAGHDLRYAIDSSKLQRELGWVPSLQFEEGLEKTVDWYLANEEWLEN
                     IINGEYQNYYKKQYEER"
     gene            3663..4601
                     /locus_tag="PKH58_06115"
     CDS             3663..4601
                     /locus_tag="PKH58_06115"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX88636.1"
                     /translation="MKLFTFATMERIARHIETLIRRHDYVIVPDFGGFVIQNQHAVIH
                     AESIEPPLATVSFNPLLNVSDGLLTIEISRAEKISFREASQLIEKEVAGLKLTLKNQK
                     TVSCGNLGTLSVDAEEKIIFKPSLNGYLLPANYGLDTLHYSPFAESHVQEERKLVITL
                     PPVRKIARYAAVGLIAVSILLAAPTLNDARQNIAGLNPASLLALSEKTIEQQNTPVAT
                     QAATVNPATEATTATPTETAKYHVVVASLPTQEMAEKFCGELKAGNFTKTHILHPSKL
                     YRVVIESFTSKDSAVTFMENLRNSAPEFSEAWVLCE"
     gene            4697..5233
                     /locus_tag="PKH58_06120"
     CDS             4697..5233
                     /locus_tag="PKH58_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNX88637.1"
                     /translation="MPTKIRLQRHGRKGYAYYHIVIADSRAPRDGKFIERIGSYNPNT
                     NPATIDLKFDRALYWVNAGAQPTDTTRNILSDEGVLLKKHLQGGVKKGAFSEEEAEKR
                     FAAWKANKENAVSKTKEQLAAEKQADLKARAKAEAEINKAKAAELAKKKAEELEAKVA
                     KAAEAEPQTETPEVAAAE"
     gene            5387..6538
                     /gene="wecB"
                     /locus_tag="PKH58_06125"
     CDS             5387..6538
                     /gene="wecB"
                     /locus_tag="PKH58_06125"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNX88638.1"
                     /translation="MKILLVFGTRPEAIKMAPLVKEFELNADKFETVVCVTGQHRQML
                     DQVLDLFGIKPDFDLNIMQSGQDLYDITSRVLTGMRDVLDKTKPDLVLVHGDTTTSMA
                     TALAAFYQRIDVGHVEAGLRTHNLYSPWPEELNRQLTGRIAKYNFSPTELSKQNLLKE
                     NVDESSIYVTGNTVIDALHSVTEKINSDVTILSEVEKQIKEAGYDTDRLISGKKMVLI
                     TGHRRENFGEGFANISEALKILSHKYPEVDFVYPVHLNPVVRGSIAKVFNDNQPDNLY
                     LIEPLDYLPFVCLMEKSNIVLTDSGGIQEEAPGLGKPVLVMRNTTERPEAVDAGTVKL
                     VGADSSKIITEVSRLLEEPEAYNAMARAVNPYGDGMASKRILEIVKDVL"
     gene            6548..7888
                     /locus_tag="PKH58_06130"
     CDS             6548..7888
                     /locus_tag="PKH58_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNX88639.1"
                     /translation="MNLSGIKKYVKNQVQKSDLSKRLYKGISWTLIGNIIGKFLQLVA
                     FIFVARIVGKEEYGQVGIIRSTLNMFLLFSSAGMGATATRYIAIYRTSDPNRAYQIYR
                     FSLKVVLAIGFAVSLIVFLMSDHIAEKYLNDVNLGDALRVGAFTLFLMTLTSVQTGAL
                     NGFERFKSLGINLAANGLILFASVIAGAYLRGVDGVVAGLAITNLIYAVQLWISLRKD
                     VLGIRQADQKPDIESLSKSSIFLKFSLPAVLESVTIVAVLWWAKTFLIGIEGYGEMAI
                     YDVAEQWYFVILFIPNSMTSILLPLLINTNYNGSKKQYDKLLNVNLLLNTGIALIISL
                     VVYLFLPLIFRFYGKGFNDFRPLIVLLVAVVICAANNVVGQIIVSKGKMWIGFGVNVL
                     RGFWLVLFTFLFVGHYRLGALGLAYAFLLSYILHSIVQGFIAVRIKNHNFDNII"
     gene            7945..9168
                     /gene="wzy"
                     /locus_tag="PKH58_06135"
     CDS             7945..9168
                     /gene="wzy"
                     /locus_tag="PKH58_06135"
                     /inference="COORDINATES: protein motif:HMM:NF033860.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HNX88640.1"
                     /translation="MDASHTHKHARWILYLFILFNVISYLYVVFTGKYNGDYLGTIPR
                     SSQSTLFIYLIYTILPYIALYLIYGHYAKMKKNNKITIPYKSFGWFLLLVILFQIITT
                     EIYGVSKLGQEYYEAPAVVKLLIQIFNRFNAILGVSIYSVVAPKKNKFNYLLWILVFI
                     LSFERASFMVFAITGLMFILLYFGSIWGFIKKRLILSIVLIIISPIAVTTLYNLRNQI
                     RINSNKIELEIDQDQNSTFSKVIFGRLTGRLSSYSNSVIIHEQKVKIKELVRYFSILQ
                     YPTEAFSAVYGKVINPKEIAYRNLQYESFGNHSRTYSAMNGTLGALLIGYYQSPLVFL
                     INLSTLLLIVVLTFELFALFHNRKLFDMIFLFFSFNVLSGNAEEYMVTLMVTFLYVVI
                     FLCLNLIEVPKALNN"
     gene            9465..10235
                     /locus_tag="PKH58_06140"
     CDS             9465..10235
                     /locus_tag="PKH58_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNX88641.1"
                     /translation="MLGMPAKNTLITIHDLGIFNNSKGLKRIFFYYFWYYLPIKKARY
                     VTCISEFTKEEIAKKIKCNIDKIKVIHNPVPLSYTYSPKEFNEINPRVLHIGTRPNKN
                     LERVIEALEGIKCHLRIIGKLTSEQEKLLSEKKIKYSNGINLTDEEILNEYILCDIVS
                     FPSLYEGFGMPVIEAQAVGRVVLGAEIEPLIEISNRAMCFVNPYDSESIRKGFKELIS
                     SQYYRNNLIEKGLENVDKFKPDAIANQYYKIYKSIAQS"
     gene            10232..11323
                     /locus_tag="PKH58_06145"
     CDS             10232..11323
                     /locus_tag="PKH58_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNX88642.1"
                     /translation="MKILLDNIIFSIQKTGGISVYWSELLKRMLSDVEFDFQCYEMNN
                     AVSNFFRAKLEIDPEKIIRENKLIGQLNRIFYVVRSTKEPYIFHSSYYRPLVTTKNAC
                     IVTTIHDFTPEIIESGWKKRAVAIQKYNSIKNADLIICVSKNTKKDLLKLYPEFSKKR
                     IEVVYNGVNEAFMPLKKNDFSVKNKFQEVNLPYCIFVGKREGYKNFKFAVEYLATRKD
                     LFLVIVGGGELSKNDFDLLNSKLSNRYAIRLFLSVEELSLLYNFAEFLFYPSDYEGFG
                     IPVLEAQKCGCPIIAQRTSSIDELIQDKNLLFPTLNFESIDVVRSYIAHNKEKIVYEG
                     IENAKQFTWDKTYEQHKHLYRTLYHKKFD"
     gene            11344..12099
                     /locus_tag="PKH58_06150"
     CDS             11344..12099
                     /locus_tag="PKH58_06150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX88643.1"
                     /translation="MKVSIITVTYNSEKSLQRSIDSVVSQDYPDIEHIIIDGGSTDNT
                     VSIIKNNKEHISTYISEKDSGIYQALNKGIRLATGDIVGILNSDDVFADTNIISKVVG
                     NFNNNSSDVVYGNLLYVTKNLSENKTIRYWKSNPYNPKFLVFGWMPPHPTLFCRKEVF
                     DTYGYYNESLQISSDYDFILRIFKEPMLKKSYIPEVMVKMTAGGKSNGTLKNICRKSH
                     EDLRAIRENKIGGIYTLFFKNVRKLKQLIRVYH"
     gene            12111..12917
                     /locus_tag="PKH58_06155"
     CDS             12111..12917
                     /locus_tag="PKH58_06155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX88644.1"
                     /translation="MLNASIVIYKHTFDEISLLVKILSVHESVHKIYLIDNSPTRTDN
                     FLKLPVEYIFNGRNLGYGAAHNIAIRKTIDQDIPYHLVVNPDIEFEASVLVEMLNFMN
                     EQTNVGLLMPKVLYPNGDTQFLCKLLPTPDDLLIRRFIPARLTKKRTSYFELRDSGYN
                     RIMDVPYLSGCFMLIRTQALKEVGLFDERFFMYPEDIDLTRRIHQKYRTVFYPHASII
                     HHHAQASYKNVKLLLIHIRNLIKYFNKWGWFFDAERKKVNRETLNQLKKM"
     gene            12954..13529
                     /locus_tag="PKH58_06160"
     CDS             12954..13529
                     /locus_tag="PKH58_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNX88645.1"
                     /translation="MNFDIQQIISAFIAVFVIIDILGSIPIIMGIKDKGEIIKPLNVC
                     IVSLGIFLLFLFGGEGILRLFKVDFESFAVAGSLIIFVYALEMVLDIEIFKNKGPEGS
                     TSIVPLAFPLVAGPASFTTILSIRAEYSILNIIIAIILNLIFVYFVLRSTEKIEKIFG
                     KGGIYIMRKFFGIILLAISVKLFMGNIGSML"
     gene            complement(13652..15055)
                     /gene="xylE"
                     /locus_tag="PKH58_06165"
     CDS             complement(13652..15055)
                     /gene="xylE"
                     /locus_tag="PKH58_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HNX88646.1"
                     /translation="MNTQQGSKFYIFGITLVATLGGLLFGYDTAVISGAVDPLKMFFH
                     LDAATYGDLANFYHGATVSSALIGCILGSAVSGIFASGFGRKKSLLIASVLFFISAVG
                     SGFPEMIFFSSKTDISTLLVSFNVYRIIGGVGVGLASAICPMYIAEMAPADIRGKLVS
                     WNQFAIIFGMLVVYFVNFFIIKGKSIDWINITGWRYMFFSESFVAALFGVLLFFVPET
                     PRYLALSRKDDKALHILSKINGAEKASVILKEIKETAHEKVEKVLSYGWKVLIIGILL
                     SVFQQVVGINVVLYYAPTIFKGLGFGNDAAMWQTVIMGVVNIIFTMVAIFTVDKFGRK
                     PLLMIGSIGMAIGMFAIGTLAYANIIGVSTLVFIIIYTASFMMSWGPICWVLISEIFP
                     NTIRGKAVAIAVAAQWISNFVVSSTFPSLAAFSIPFTYFLYGVMSVLSALFVWRMVPE
                     TKGKTLEDMSKLWKINN"
     gene            complement(15198..>15559)
                     /locus_tag="PKH58_06170"
     CDS             complement(15198..>15559)
                     /locus_tag="PKH58_06170"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444220.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNX88647.1"
                     /translation="LTQAMLVILQGGGMQGGGTNFDAKIRRNSTDNEDLFIAHIAAMD
                     VMARALETAAAILEKSPYKKLVADRYASYDSGKGKEFEEGLLSFEDCVAYAKSKGEPK
                     QISGKQELYEAIVNMYI"
BASE COUNT         4839 a         3000 c         2981 g         4739 t
ORIGIN      
        1 actgatcact gatcactgat tactgatcac tgattactcg ttctccgtta tttcagctcg
       61 tcaaattttg ctttcattgt cgggttgttg tacgtcagtt ttttaatggt taagtcttcc
      121 gttttttcgt tggcaagccg aagattgttt tctgatgaca acaaagcgtc tttggttttt
      181 tgtaattccc tgatcgtttt atctattcca tcaatcgcgt ctttaaactt acggctggct
      241 aaatcataat ttcttgcaaa cccgtctttg aatttattca tcttctcctc aaaattggta
      301 atatcgatat tctgacttct taccagcgcc agttctgcct tatactgaat ggtattcatg
      361 gcggcattgc ggagtaaagt tatgattggg atgaaaaact gtggtcttac aacatacatt
      421 ttattgtacc tgtgcgatac atcaacaatc cccgtattgt aatattcact atcggcttcg
      481 agcaatgtta ccagaacggc atattcgcat ttcttttcgt tccggtcctt gtccagttcc
      541 ctgaaaaaat cctcattctt cttttttgta gcggtttcgt ccccctcatt tttcatttca
      601 aacatgatgg aaataatttc gtttccgaca tcgtctgttt cccggtaaat gtaatctccc
      661 ttgctgcccg atcttgcatc gttgtctttt tcaaaatatg ccttctgaaa cgctgtcgcg
      721 cgaagtttgt tgaactcggt ctcacaatgc tgttcaagcg tttcacccag cattttggtc
      781 gagagtttca gcttcatatc tttgacacgc gctatttcct catccttgta cttgattatc
      841 gcatccttgt tttgcagttc agttgaaaat ttttgcttca acgtactttc aatattctgt
      901 ctttccagtt ccttcgtctt aatatcatta gccaactggt cccgttcttt ctccagtttc
      961 tgaaccgcct cggtaacagc aagctttttc tcaacctcaa cattttcaat cttcgctttt
     1021 agctgcgtta tttcatactc ttttttattc agcttctccg tcagcgccaa ctcgctttgg
     1081 gctttgagtt ctgccagttc cctgtccttt cgggccagtt gttcctgcag ggaattacgg
     1141 atatttgctt ccgccagttt tacggcactt tctttctctc tgtcagccag actaagccgg
     1201 tttttcagtt cttcttcaaa ctgacggtcc cgaacttgct ttagaatatc ggcaaaaccg
     1261 gcttcatcca ctttaaaagc tttcttacag ttggggcaaa taatttcgtt catatctgtt
     1321 ctgtttgatg tttgttcttt aatatttcat ataacttttt atttcttcca gcacttgcat
     1381 ggggtaatta aatatccacg tattttcaaa ccggataacg gtttgtgaaa atactttatt
     1441 tgtttcttcc atggttttat caatcatccg gtattctgtc tgcaactctg taaacccttt
     1501 gcttttatcg ttgatgcttt ttatacttca catccgataa acagccccta ctgtcgcgcg
     1561 atttagggta caatttcacg gcagcggggg ctgatactga tataaagctt ttgggaataa
     1621 tgttggtgaa aggattgtaa cataaacagt gatttaagtt aatagatgca caaatataca
     1681 tatattaaat tttattcaac catcaaaaca aatatttagc gtcatttttt tgaagtttcc
     1741 ttattatatc ttaaatattt tccttacagg ttcaggctgt ccctttataa atgcatattt
     1801 cagtttacaa ggtaataatt ctgtaatttc acgggtgata ttatacatca tgtaaaaccc
     1861 catctctcac ttgaggctta aatttttaca tcatgtaaaa agacagttct ggtgtagaaa
     1921 aatctgtacc atatcattta accgaaaaac gataaagaaa aaacattcat atgataatcc
     1981 atgtgacacc ctggtaaaca gttattcata accattttat gtaaatcacg gacaggaatg
     2041 tccgtgcacc cgaaatgttt gtgaaacttc gtggatgatc tttgtgaaac tttgtggtaa
     2101 agctattaca caaaagacac aaaggacaca caaaacacgc aaagaagaac cactaatgct
     2161 gttttgagga atgcaacgta aaaagaattt tctttgtgaa acttggtggt acagccaact
     2221 gaacaaatca acgaaaaaat gtttcggtac gctgtacctt aaaattcggg gattatcggt
     2281 tttctaaaaa tattacgcca cgctgtggct tgtattcagg tgcagagcac cgtgatattt
     2341 gtagcaaatg tcatttcaaa taaaatttta aggtgcagcg caccgaaaca gaacatggga
     2401 aatgctataa atccctaacc tctatcccct aaaaacaaca ccgctcctcc ggaacttatc
     2461 cgaaggagcg gcagctgtat atatttacgt caatgttggt attatctttc ttcgtactgc
     2521 tttttataat agttctgata ttcgccgttg atgatgtttt cgagccattc ttcgttggcc
     2581 agataccagt caaccgtttt ctccaaacct tcctcgaact gaagcgaagg tacccagccc
     2641 agttcacgct gcaacttcga cgaatcaatg gcgtaacgaa ggtcgtgacc tgcccggtcc
     2701 tttacatacg taataagttg agccgattct cccggtgcgc ggttgagttt gcggtccatg
     2761 atggcacaca gttcttttat caggtcgatg ttggtccatt cgttatgtcc accgatgtta
     2821 taggtttcgc cggtcacacc ctgatgaaaa atgacatcaa tagcccgtgc gtggtcatcc
     2881 acccaaagcc agtcgcgcac attctctcct ttgccataaa tgggaacagg cagtttcttt
     2941 ttgatattgt tgatagccag cgggattaat ttttcgggga aatgatgcga accgtagttg
     3001 ttggaacagt tggaaactac aacaggcaga ccgtaagtat gataataggc acggacaaaa
     3061 tggtcggaac ttgctttggc agccgaatag ggactgcggg gatcgtaggc ggtttcttcg
     3121 gtgaagaaac cttcgcttcc gagtgaacca tatacttcgt cggtggaaat atgataaaac
     3181 cgtttacctt ccatgctacc tttccaggca ttcttggcgg cattcagcag gttggctgtt
     3241 ccaaagacat tggtgcggat aaaggcaaac ggatcggtaa tggaacgatc gacatgcgac
     3301 tcggcagcca gatgtatcac cccgtcgaat gaataacggg caaacaactc cggtatgaat
     3361 ttttcgtcgg taatatcgcc ttttacaaag gtataattag gtgcatcctt tacatccttc
     3421 aggttctcca ggttgccggc ataggtaaga gcatcgaggt ttacaatctg atattcgggg
     3481 tatttggtta caaacaagcg taccacgtgt gaaccgataa atccggctcc tccggtaatt
     3541 aaaatggttt ttcccataat attattttgt tctgaatttt ggtataattt gtacaaagat
     3601 acattctttg ggtgatactt aaaacttgtt gaaaactttt tcggcagaaa ttgttttttg
     3661 atatgaaatt atttactttt gcaacaatgg aaagaatagc ccgacacata gaaacactga
     3721 tccgtcgcca cgattatgtt atcgtgcccg attttggtgg ttttgtcata caaaatcagc
     3781 acgcagtcat tcatgccgaa agtattgaac cgccccttgc aacggtaagt ttcaatcctt
     3841 tgctgaatgt ttccgatggg ttgctcacca ttgaaatctc ccgtgccgag aaaatcagtt
     3901 tccgggaagc ctcgcagctg atagaaaaag aagtcgccgg actgaaactg acattgaaaa
     3961 atcaaaaaac agtcagttgc ggtaatctgg gcaccttatc ggttgacgcg gaagaaaaaa
     4021 tcattttcaa accctcgctt aacggatatt tacttcccgc gaactatggt cttgacaccc
     4081 tgcactactc tccgtttgcc gaaagccatg ttcaggaaga acggaagctg gtgataacac
     4141 ttcccccggt acgaaaaatt gcccgctatg ctgccgtagg gctgatagct gtcagtattc
     4201 tactggctgc acctacgctg aacgatgccc gtcagaacat tgccggatta aatcctgcaa
     4261 gtctgcttgc tctttccgag aagacaatag aacaacaaaa caccccggtc gcaactcagg
     4321 cagccactgt aaacccggca actgaagcca caaccgcaac gcctacagaa acagcaaaat
     4381 atcatgtggt ggtggcaagt ctgcccacac aggaaatggc cgaaaaattc tgtggggaac
     4441 ttaaagcggg aaactttaca aaaacccaca tcctgcaccc ttccaaatta taccgtgtcg
     4501 tgatagaatc gttcacctcg aaagattctg cagtgacctt tatggaaaat ctgagaaaca
     4561 gcgcccctga attttcagaa gcatgggtac tctgcgaata atcctgttaa atttttctgt
     4621 ttcaaaaaaa taatactatc tttgcaaccg cttttcgaga aaaagcaata ggtataatca
     4681 tttaaaaaga aaaaaaatgc caacaaaaat cagattgcaa cgtcatggtc gcaaagggta
     4741 tgcttattac cacattgtga ttgctgatag tcgcgcgcca cgtgatggca agtttatcga
     4801 acgaatcggt tcgtacaatc cgaacaccaa tcccgctacc attgatctga agttcgacag
     4861 agctttgtat tgggtaaatg ctggtgctca acccacagac acaacccgca acatcctctc
     4921 tgacgaaggt gtgctgctga aaaagcacct gcaaggtggt gttaaaaaag gagctttcag
     4981 tgaagaagaa gccgaaaaac gcttcgctgc ctggaaagca aacaaagaaa atgctgtaag
     5041 caaaaccaaa gaacaattag ccgcagaaaa acaggctgat ttgaaagcac gcgctaaagc
     5101 cgaagctgaa atcaacaagg caaaagctgc tgaactggct aagaaaaaag cagaagaact
     5161 ggaagcaaaa gtagctaaag ctgccgaagc agagccacaa accgaaacac cggaagttgc
     5221 tgctgctgaa taagttatct gcaacgatat tttatttata aaaagcccga ataattaatt
     5281 cgggtttttt tattaaaatg tgctgagaag tgcaatttta atgttaaaaa ttcgttgaaa
     5341 aaaaaatgct gttaccatat tgaatataca attacccgtg aaaagaatga aaatcctcct
     5401 cgtgtttggt acacgacccg aggctatcaa aatggctcct ctggttaaag agtttgaact
     5461 gaatgctgac aaatttgaga ctgtagtatg tgttaccggt cagcatcggc aaatgctcga
     5521 tcaggtattg gatttattcg gcatcaaacc cgattttgat ctgaatatca tgcaatccgg
     5581 acaggatctg tatgacatca caagccgcgt tttaacaggg atgagagatg tgctggataa
     5641 gacaaaacct gacttagtct tggttcacgg cgatactacc acttctatgg caaccgccct
     5701 ggctgctttt tatcagcgaa ttgatgtagg acacgttgaa gccggacttc gaactcacaa
     5761 tctctacagc ccctggcccg aagaactcaa cagacaactc accggccgga ttgcaaaata
     5821 caatttttcg cctaccgaat taagcaaaca aaacctgctg aaggaaaatg tggatgaatc
     5881 ttccatatat gttaccggca atacggtaat tgatgccctg cacagtgtaa ctgaaaaaat
     5941 aaattcagac gttaccattt tatcggaagt tgaaaaacaa attaaagaag cgggatatga
     6001 tacagacaga ttaatatccg gaaaaaaaat ggtattaatt acaggtcacc ggagagaaaa
     6061 ctttggtgaa ggctttgcaa atatatccga agcattaaaa atactctcgc ataagtatcc
     6121 tgaagttgat tttgtttatc ctgtacacct aaatccggta gttcgtgggt caatagcaaa
     6181 agtctttaac gataatcaac cagataacct gtatctgatt gaaccgctgg attatctgcc
     6241 gtttgtctgc ctgatggaaa aaagtaatat tgtactgaca gacagtggcg gcattcagga
     6301 ggaagcgccc ggattaggca aaccggtttt ggttatgcga aataccaccg aacgtccgga
     6361 agcggtggat gccgggactg taaagctggt aggcgccgat tcgtccaaaa tcattacgga
     6421 agtatcccgg ttgctggaag agcctgaagc ttataacgct atggccagag cagttaatcc
     6481 atacggggat gggatggcat cgaaaagaat tttggaaata gtgaaagatg ttttataatt
     6541 gaggagcatg aatctatccg gaattaagaa atacgttaaa aaccaggttc aaaaatctga
     6601 tctttcaaaa agactgtata aaggtatttc gtggacttta ataggaaata ttattggcaa
     6661 atttctgcaa ctggtagctt ttatttttgt tgccagaata gtaggaaaag aagaatacgg
     6721 gcaggttggc attattcgtt ctacgctaaa tatgtttcta ctattttcat cagcaggtat
     6781 gggtgcaaca gctacgagat acattgcaat ttacagaaca tcagatccca acagagcata
     6841 ccagatttac cggttttcct tgaaagtggt tttggcaatc ggttttgctg tatctttaat
     6901 agttttcttg atgagcgacc atatcgctga aaaatactta aatgatgtca accttggaga
     6961 tgcattgaga gtaggagctt ttacactttt tctaatgact ttgacatcgg ttcaaaccgg
     7021 tgctttaaat ggttttgaac ggttcaaaag cctcggaata aatttagctg caaatggatt
     7081 gatattattt gcttctgtaa tagccggggc atatttgaga ggagtagatg gggttgttgc
     7141 cggactggct ataactaacc tgatttatgc tgtacaactg tggatttcct tgagaaaaga
     7201 tgttctgggt atacgtcagg ctgaccaaaa accagatatt gaaagtctga gcaaatcatc
     7261 tatttttttg aaattctctt taccggcagt tctcgaaagc gtaacgattg ttgccgtgtt
     7321 gtggtgggca aaaacgtttt taattggcat agaaggatac ggtgaaatgg ccatttatga
     7381 tgtagccgaa caatggtatt ttgttatctt atttatacca aattctatga catcgatact
     7441 tctgcctctt ttaataaata cgaactacaa tggttcaaaa aaacagtacg ataaactatt
     7501 gaatgtcaat ttgttactaa atacaggaat tgctttaata atatctcttg ttgtttatct
     7561 gtttctacct ctgatattta gattttacgg caagggtttt aatgattttc gtccattaat
     7621 agttcttttg gtcgctgttg taatttgtgc agcaaataac gtagttggtc agataatagt
     7681 gagcaaaggt aaaatgtgga ttggtttcgg tgtgaatgtg ttaagaggat tttggcttgt
     7741 attatttaca tttttatttg tggggcacta caggcttggc gctttaggat tggcttatgc
     7801 atttttatta agctacattc ttcatagcat agtgcaaggt tttattgctg ttagaataaa
     7861 aaatcataac tttgacaata taatatgacg actcattggc aaggtccaac caaaaaacaa
     7921 taacagagca acaaacaaga atttatggat gcttcacata cacacaaaca tgcaagatgg
     7981 atattgtatt tgtttatttt gtttaatgtc ataagttacc tttatgttgt tttcacagga
     8041 aaatacaacg gcgattattt aggtacgata ccaagatcct ctcaatcaac attgtttata
     8101 tatctgatat acaccatctt accatacatt gcattatatt tgatatacgg gcattatgca
     8161 aaaatgaaaa aaaacaataa aataaccatt ccctacaaat cttttggatg gtttttgttg
     8221 ctggttattc ttttccagat tatcactact gaaatttacg gagtaagtaa attaggacaa
     8281 gaatattacg aagcacctgc agttgtcaaa ttgctcattc agatattcaa ccgcttcaac
     8341 gcaattcttg gcgtatcgat ttattcggtt gtcgcaccaa agaaaaataa gttcaattat
     8401 ttgctttgga ttctggtatt cattctttcg tttgagaggg catcttttat ggtgtttgct
     8461 attaccggat taatgtttat acttctatat ttcggttcta tttggggttt catcaagaag
     8521 cgtttaattt tgtccatagt attgattatc atatccccta tcgccgttac cacgctttat
     8581 aatttacgaa atcaaataag aataaatagt aataaaattg aattagagat agaccaggac
     8641 cagaacagca ctttttcgaa ggttattttt ggacgcctga caggcagatt atcttcttat
     8701 tcaaattcag taataattca tgaacaaaaa gtaaaaatca aagagttggt acgatatttt
     8761 tcaattttgc agtatccgac agaagctttt agtgctgttt atggcaaggt tatcaatccc
     8821 aaagaaattg cttaccgaaa tttgcagtat gagagctttg ggaatcacag caggacctac
     8881 agcgcaatga atggcacact aggggcacta ctaattggat attatcaatc acctcttgtg
     8941 tttcttatca acttgtcaac gttattgctt attgtggtgc tgacttttga actgtttgct
     9001 ctctttcaca atcgaaaatt atttgacatg attttcttat ttttttcgtt taacgtgttg
     9061 agcggcaacg ctgaagaata tatggtaacg cttatggtaa catttttata cgttgtaatt
     9121 tttctgtgct taaatcttat tgaagttcct aaagctttaa acaattaata aaataaagta
     9181 tcggctaata caataacatt aacagtttat tttactagca tttgaatatc ctggacatga
     9241 cagattttga cgaaataaaa attacatatt tttttaggaa aaaaaattac gaacatagta
     9301 ttgagcgggt gtttgataat gtgaaacacg gattaaaaat ttcgtattca gataaatatg
     9361 tgccgtatat taaagttaca cctgttcagc tcctcaaaaa tattatctat tgcaggaata
     9421 attcagggaa aattaaccac ataacgggcg acatttatta tgctatgctg ggtatgcctg
     9481 ctaaaaacac attgattaca attcatgatt taggcatttt caacaacagc aaaggcctaa
     9541 agagaatttt tttctattat ttttggtatt atttaccaat aaaaaaagcc aggtatgtta
     9601 cctgcatttc tgaatttacg aaggaagaaa ttgcaaaaaa aatcaagtgt aacatcgata
     9661 aaattaaggt cattcacaat cccgtaccac tatcatacac ttacagccct aaagagttca
     9721 acgagataaa tccaagagtg ttacatatag gaacacgacc gaataagaat cttgaaaggg
     9781 taatcgaagc acttgaaggt attaaatgcc acctaagaat aattggcaag cttaccagcg
     9841 aacaggaaaa attgctttct gagaaaaaga taaagtattc aaacggtata aatcttacag
     9901 atgaggaaat attaaatgaa tacatcctct gcgacatagt cagttttcct tctctttacg
     9961 aggggtttgg tatgcctgta atagaggcac aggctgtcgg cagggttgtg ttaggtgctg
    10021 aaattgaacc tcttattgaa atttccaata gggcaatgtg ttttgttaat ccttatgatt
    10081 cagagtcaat acgcaaagga tttaaagagt tgatttcgtc acaatactat cgtaataatt
    10141 taatagaaaa aggactggaa aatgttgaca aatttaagcc ggatgcaata gcaaatcagt
    10201 actataaaat atataaatcc attgcgcagt catgaaaatc ttacttgaca atattatttt
    10261 ttctattcaa aaaaccggcg gaatctctgt atattggtct gaattgctga agaggatgct
    10321 ttctgatgtc gaatttgatt ttcagtgtta tgaaatgaat aatgcagtat ccaatttttt
    10381 tagagcaaag ttggaaattg accccgaaaa gataattcgt gaaaataaat taataggaca
    10441 attaaacagg atattttacg ttgttaggtc aacaaaagaa ccgtacattt ttcattccag
    10501 ctattaccgt cctttggtta cgaccaaaaa tgcttgtata gtcacgacta ttcacgattt
    10561 cacacctgaa atcattgaaa gtggctggaa aaaaagagct gttgctattc agaaatataa
    10621 ttcgataaag aatgcggatt taattatttg tgtatcaaaa aacacaaaaa aggatttatt
    10681 aaagctttat cctgagtttt ccaaaaagag aattgaagtt gtttacaacg gagtaaacga
    10741 ggcgtttatg ccgctgaaaa aaaatgattt ttccgttaaa aataaatttc aggaagtaaa
    10801 tcttccctat tgtatttttg ttggtaaaag agaaggatat aaaaatttta aatttgcagt
    10861 tgaatatctt gcaacaagaa aagatttatt tctcgtaata gttggaggcg gcgaactgag
    10921 taaaaatgat tttgatctgc ttaattctaa attatcaaac agatacgcca taagattatt
    10981 tttatctgtt gaagaattaa gtttgctgta caattttgct gaatttttat tttatccttc
    11041 tgattatgag ggttttggta tccctgttct cgaagctcaa aagtgcggat gtcccattat
    11101 agctcaacgt acatcatcca tcgacgaact gatacaggat aaaaatttac ttttcccaac
    11161 gcttaatttt gaaagtattg acgttgtgag aagctacatt gcccacaata aagaaaaaat
    11221 cgtttacgaa ggcattgaaa atgcgaaaca gtttacatgg gacaaaacct atgaacagca
    11281 taaacatttg tatcgtactc tataccataa aaagtttgat taatttttca agacttcttt
    11341 tccatgaaag ttagcattat tacagtaaca tacaacagcg aaaaatctct gcaacgttcc
    11401 atcgattccg tcgttagtca ggattatcct gatatagaac acattattat tgacggtggt
    11461 tcgacagaca atactgtcag cattataaaa aataacaagg aacatatatc aacatatatt
    11521 tcggaaaaag acagcgggat ttatcaggcg cttaataagg gcatcagact ggcaactggc
    11581 gatatcgtcg gaattctgaa ctctgatgat gtgtttgctg atacaaatat tatctcaaag
    11641 gttgtcggaa atttcaacaa caacagcagc gatgtggttt acggaaatct gctctatgta
    11701 accaaaaatc tgtcggaaaa taaaacaata cgttattgga aaagcaatcc ttacaatcct
    11761 aagtttcttg tatttggctg gatgccgccc catcctaccc tattctgcag aaaagaagtt
    11821 tttgatacct atggatatta caatgaatcg cttcaaattt cttccgatta tgattttatc
    11881 ctccgcattt ttaaagaacc aatgttaaag aaaagctaca ttccggaagt tatggtcaag
    11941 atgacagccg gaggcaagag taacggtacc ttaaaaaata tttgccggaa atcgcacgaa
    12001 gatttgagag ccatccgaga aaacaaaatc ggcggcattt atacgctgtt tttcaaaaac
    12061 gtacgaaagc taaaacaatt aataagggta tatcattaat caaaaaatag atgttgaatg
    12121 cttccattgt aatatataag catacattcg atgaaatatc gttactggtc aaaattcttt
    12181 cagttcatga aagtgtacat aaaatttatc tgattgataa ttcgccaaca cgtactgata
    12241 attttcttaa actgcctgtt gaatacatct tcaacggtcg gaatctcggt tacggagctg
    12301 cacataacat agcaatccga aaaaccatag accaggacat cccataccat ttagtggtca
    12361 atccggatat agagtttgaa gcatccgtgc tggttgaaat gctaaatttc atgaatgaac
    12421 aaaccaacgt gggtctattg atgccaaaag tattgtatcc gaatggtgac actcaatttc
    12481 tttgcaagtt gctccctact cccgacgatt tgttgatcag gcgtttcatt ccggcaagac
    12541 taacaaaaaa acgaacctcg tatttcgaac tgagagattc agggtataac cggatcatgg
    12601 atgtacccta tttgtcaggc tgttttatgc ttataagaac tcaggcattg aaagaggtag
    12661 gcttgtttga tgagcggttc tttatgtacc ctgaagatat cgatttaacc cgccggattc
    12721 atcagaaata ccgtaccgtt ttttatccgc atgcaagtat cattcatcat cacgctcagg
    12781 catcatataa gaatgtaaaa ctattgctga tacatatccg taatctgata aaatatttca
    12841 ataaatgggg ttggtttttt gatgctgaac gaaaaaaagt gaaccgggaa acgctgaatc
    12901 agctgaaaaa aatgtaattt tgcaagaaac aagaatatta attcaacacc gctatgaact
    12961 tcgacattca gcagattatc agcgctttta ttgcagtttt tgttatcatc gatatcctgg
    13021 gctccatacc cattatcatg ggcattaaag ataaaggtga aattatcaaa ccgttaaatg
    13081 tatgtattgt ttctttgggc atttttttac tgtttctgtt cggaggtgag ggtattttaa
    13141 gattattcaa ggtggacttc gagtcgtttg cggtagccgg ttcgcttatc atttttgtgt
    13201 atgccctgga aatggtgctg gacattgaga ttttcaaaaa caaaggaccc gaaggcagca
    13261 cttcgattgt tccgctggct ttccccctgg tggctggtcc ggcgtcattt accactatcc
    13321 tttccatccg cgccgaatat tcaattctaa atattattat tgcgataata ttaaacctga
    13381 tttttgtgta ctttgtactt cgatcaactg agaaaattga aaagatattt ggtaaaggcg
    13441 gtatttatat catgcgtaag tttttcggta ttatcctact ggccatctcc gtaaaattat
    13501 tcatgggaaa tatcggctcg atgctgtaaa tgaataagta ccacaaaaat tacggtgcgc
    13561 tatcccttaa tggagtagcg caccgtaaat atttggtgat atttgaacag gttaaactaa
    13621 gtcagtgaag ggaataattc tttttccttt atcaattgtt tattttccac agtttactca
    13681 tatcttccag tgtttttcct ttggtttcag gcaccattct ccacacaaac aaagctgata
    13741 acactgacat tacaccatac agaaaatagg taaacggaat gctgaaggca gctaatgacg
    13801 ggaaagtgga tgaaaccaca aaatttgata tccactgagc tgcaacggct attgccacag
    13861 ctttcccccg gatggtattc gggaaaattt cggaaataag tacccagcaa atcggtcccc
    13921 aggacatcat aaaggaagct gtgtaaatga tgataaacac aagtgtactc acaccaatga
    13981 tgttggcata ggcaagcgta ccgatggcaa acataccaat tgccataccg atggagccaa
    14041 tcatcagcag cggtttgcgt ccgaacttat caactgtaaa aatggcaacc atcgtaaaga
    14101 tgatattgac aactcccatg ataactgttt gccacatggc agcatcattg ccgaaaccaa
    14161 gacctttgaa tatagttggt gcatagtaga gtaccacatt gatgcctacc acctgctgaa
    14221 acacagaaag aagaatgcca ataatcagga ctttccagcc ataagaaaga accttttcaa
    14281 ctttctcgtg agcagtttcc tttatttctt tcagaatgac cgaagctttt tccgcaccat
    14341 ttattttact cagaatatga agagctttat cgtcttttcg ggataaagca agataacgcg
    14401 gagtttcggg gacaaaaaac aataaaacac caaacaaagc agccacaaaa gattctgaga
    14461 aaaacatata tcgccagccg gtaatattaa tccagtcgat tgattttccc ttgatgatga
    14521 agaagttgac aaaatatacc accagcatac caaagataat ggcaaattga ttccacgaaa
    14581 caagctttcc tcgtatatca gctggcgcca tttcggcaat atacatcgga catatggctg
    14641 aagccagacc tacacccact ccgccgataa tccggtaaac attaaatgag acgagcagag
    14701 ttgaaatatc ggttttactg ctgaagaata tcatttcagg aaaacctgaa ccaacagctg
    14761 atatgaagaa taaaactgac gcaatcaaca gtgatttctt acggccaaaa ccggacgcaa
    14821 aaatacctga aacagcgctt cctaaaatac aaccaatcaa tgcactggac acagtagcac
    14881 cgtgatagaa atttgccaga tcgccataag tagcagcgtc gaggtgaaaa aacattttca
    14941 atggatcaac cgcgcccgaa ataaccgccg tatcgtaccc gaaaagcagt ccgcccagag
    15001 tagctaccag cgtaatcccg aaaatataaa atttacttcc ttgttgtgta ttcattattt
    15061 tttagattta agagcaaaga gcaaagaata aagacgaaag agcaaaaact gaaaaataaa
    15121 gatttttggg aattacttcc aatattcttt accgttgcat ttgttgtctt ttcttttacc
    15181 tttgctcttt tatctgatta gatatacata tttacaattg cttcgtatag ctcttgtttt
    15241 ccactgattt gttttggttc acccttagat ttagcataag caacacaatc ttcgaaagat
    15301 aataatcctt cttcaaattc tttaccttta cctgagtcat aagatgcgta acggtcagca
    15361 accaatttct tatatggaga tttttcgaga attgcagcag cagtttccaa tgcacgagcc
    15421 ataacatcca tagcagcaat atgtgcaatg aataaatctt cgttatcggt tgagttacgg
    15481 cggattttag catcgaagtt ggttccgcca ccctgcatgc caccgccctg aagaataacc
    15541 aacatagctt gtgtcaatt
//
LOCUS       DAOPYB010000058        15287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4182_length_15287_cov_9.586200, whole genome shotgun sequence.
ACCESSION   DAOPYB010000058 DAOPYB010000000
VERSION     DAOPYB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15287
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4182_length_15287_cov_9.586200"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKH58_06175"
     CDS             <1..324
                     /locus_tag="PKH58_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88648.1"
                     /translation="TGILNYCIEAGVKGILCFGFGMTLREGDREYYYSKLDEHFPGLK
                     EKYQHKYGNAYEITSDNNPKLMKIFNETCSANHIMTDVNSIFSFLHEFPEKNRNSQLD
                     LFGQE"
     gene            553..1653
                     /locus_tag="PKH58_06180"
     CDS             553..1653
                     /locus_tag="PKH58_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNX88649.1"
                     /translation="MTALGTFELNNIYQGDCVELLNKIPDNSVDLIFADPPYNLQLNG
                     DLYRPDLSKVDAVDDEWDKFDSFGEYDQFTNQWLAECHRVLKPTGSIWVIGSYHNIFR
                     VGTALQNIGFWILNDIIWVKSNPMPNFKGTRFNNAHETLIWATKSKSSKFTFHYHSMK
                     VMNDDLQMRSDWVIPICQGNERIKVNGMKAHSTQKPEELLYRVLISSSNPGDVVLDPF
                     SGSGTTAAVAKRLGRRYIAFERDEFYIRVARERLEKITPVDGKITGYPIENRIPKVPF
                     NSLIVEDYIRLGELLYSKDGKVSATVNADGTLLHDGFVGSIHKVSAKILNRSNNNGWS
                     FWYVMRDNKLVSIDTLRYEYEKKYIKRIQHKL"
     gene            1664..2302
                     /locus_tag="PKH58_06185"
     CDS             1664..2302
                     /locus_tag="PKH58_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88650.1"
                     /translation="MSYSLINPGVYDCKCCPTRCDGITVPKFDFGRDTAFSEAVENEV
                     INYINRNYPALFAVKTSQEGYPDIEVSRIDSSQVCLYIEIKVQSRTFMSVRKYLPDAG
                     LYPSETLALNLSDLERYFVVKENEKVPVFITWCLLNRPCVVGVEPGGKCFFSQNLDEL
                     QKIRLMDNNNYRRFRRASGDGDMVNGVHKGVVVNYHFSIRELFNGLPDLNKL"
     gene            complement(2401..3270)
                     /locus_tag="PKH58_06190"
     CDS             complement(2401..3270)
                     /locus_tag="PKH58_06190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNX88651.1"
                     /translation="MASLKEIKSRIQSVKSTQKITSAMKMVSSAKLRKAQKRIENFYP
                     YERKLTHILNNFLSAESENNSVYAEQREVKRVAILVFSSNSSLCGGFNSNVAKRLNTV
                     LERYNHLGKENILIYPVGKKIARVVKKMDYFADNFDELADKPTYPATQDLADDLMELF
                     INKSIDKVEMIYHHFNNKSSQVLVDETLLPITLEVSNEKNTGRQLNYIVEPDRINIMT
                     DLIPKVLRLKVYTALLDSNASEHAARTIAMQIATDNADDLLQELNLQYNKSRQQAITN
                     ELLDIVGGSFGQS"
     gene            complement(3414..4994)
                     /gene="atpA"
                     /locus_tag="PKH58_06195"
     CDS             complement(3414..4994)
                     /gene="atpA"
                     /locus_tag="PKH58_06195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNX88652.1"
                     /translation="MSDSIKVSEVSEVLRMQLEGIDTGVKFDEIGTVLQVSDGVVRIY
                     GLHNAEANELLEFDNGIKAIVMNLEEDNVGAVLLGPTSEIKEGYSVKRTKRVASIFVG
                     EGMLGRVINPMGEALDGRGKVTGELIEMPLERKAPGVIYRQPVTQPLQTGLKAIDAMI
                     PIGRGQRELIIGDRQTGKTSIAIDTIINQRRNFDEGDPVYCIYVAIGQKGSTVANIVN
                     TLKEHNALEYTIVVAANASDPAALQYYAPFAGAAIGEYFRDTGRHALVIYDDLSKQAV
                     AYREVSLILRRPSGREAYPGDIFYLHSRLLERAAKIINQEEVAKSMNDLPESLRDRVK
                     GGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLDTDLFNQGNRPAINVGISVSRV
                     GGTAQIKAMKKVAGTLKIDQAQFRELEAFTKFGGDMDAVTAMTIDKGQKNTRLLVQSL
                     HQPMAVEKQVAVLYCGTHGLLSKVPLDKVSEFEKEFLSTLELTHQEDVLDVIKKGIID
                     DTVTSILEKTAEEVARRI"
     gene            complement(5077..5631)
                     /locus_tag="PKH58_06200"
     CDS             complement(5077..5631)
                     /locus_tag="PKH58_06200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNX88653.1"
                     /translation="MNTGLISLRYATALLDFARETNSMEKVYGEAKLLTLMFMKMSEL
                     AIVLDNPVLPTAEKRQLILNAAGGKTSKTFEKFTDLLLKNNREAQIRNIMLSFIDLYR
                     KQKNIRYGKLTTASAVDEVTEERLMALVTNNVGGTLELEKVIDPAIMGGFILEVEDVR
                     WDASISGQLTRIKNEFIERNRRIV"
     gene            complement(5777..6277)
                     /gene="atpF"
                     /locus_tag="PKH58_06205"
     CDS             complement(5777..6277)
                     /gene="atpF"
                     /locus_tag="PKH58_06205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNX88654.1"
                     /translation="MSLLTPDLGLLFWMLIAFGAVVFVLVKFGFPIILNGVEKRKNYI
                     DESLLIAKQAREELSRVKADSESLINETRKEQAKIMAEAAKSRDLIVDGAKEKAIEEA
                     AKILEGARHQIALEKEDAIRQIRREVASLSVDIAEKVLRERLDKKNSQMDMIDRLLDE
                     INIPKS"
     gene            complement(6290..6538)
                     /gene="atpE"
                     /locus_tag="PKH58_06210"
     CDS             complement(6290..6538)
                     /gene="atpE"
                     /locus_tag="PKH58_06210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNX88655.1"
                     /translation="MLLSLLLQAAGAAGLGTLGSAVGAGLAAIGAGLGIGKIGSSAME
                     GIARQPEAAGDIRINMIISAALIEGVALFAVVVCGFIL"
     gene            complement(6605..7711)
                     /gene="atpB"
                     /locus_tag="PKH58_06215"
     CDS             complement(6605..7711)
                     /gene="atpB"
                     /locus_tag="PKH58_06215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNX88656.1"
                     /translation="MKRLLNIFSLLTVILLINATALRANEPSAVNATEHQTEESGELN
                     VKELILEHLGDTYEWHITTIGEKHISVPLPVILYSKTTGWNLFMSSAFHHGHESHNGF
                     YIAKEGDHKGKIVEKNATGEEIRPVDISLTKNAASLLISAVVLIVIILLIARSYKRNP
                     LEAKKGFVGMMEMFILSVYDGIIKPGVGKDYKKFAPYLLTVFFFIFTNNLLGLIPIFP
                     GGANVTGNIAITFVLAVCTFLIVNITGTKEYFKEIFWPDVPMWLKVPIPIMPAIEVIG
                     VFTKPFALMIRLFANILAGHSIVLGLVCLIFVTVSLGTPTNASMTVVSVIFTIFIDFV
                     ELLVAYIQAYVFTMLSAVFIGLSHIEPHHHKEVA"
     gene            complement(7708..8136)
                     /locus_tag="PKH58_06220"
     CDS             complement(7708..8136)
                     /locus_tag="PKH58_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88657.1"
                     /translation="MEKSRNKFILILTAVILITGFGLKYLTEDVMPQFKVSAFTLIPV
                     FFYIIGLTLIYVLTGINPAESKKLVNKYMLLRVIKVLTSLAFLLIFWLINKAEIKNFA
                     LLFIVFYMIYLIFETWMYLQVEKMMKKNAGVKNDLTEKTE"
     gene            complement(8143..8385)
                     /locus_tag="PKH58_06225"
     CDS             complement(8143..8385)
                     /locus_tag="PKH58_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNX88658.1"
                     /translation="MKLDIVTPEEIYFSGDVTSVTLPGTNGSFSLLENHAPFISSLGK
                     GKVVYVSGGVSKEFRVKGGFVEMSNNVITVCLEAID"
     gene            complement(8438..9961)
                     /gene="atpD"
                     /locus_tag="PKH58_06230"
     CDS             complement(8438..9961)
                     /gene="atpD"
                     /locus_tag="PKH58_06230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNX88659.1"
                     /translation="MSELVGRISQIIGPVVDVAFDLSAKEEVKLPAIHDALSINRGDG
                     RELIVEIQQHIGENTVRTVAMDSTDGLRRGLPVIVKGQPITMPVGEQVKGRLMNVIGE
                     SIDGMKQLDREGAYPIHREPPKFEDLSTTTEVLFTGIKVIDLIEPYAKGGKIGLFGGA
                     GVGKTVLIQELINNIAKGHSGFSVFAGVGERTREGNDLLREMIESGVIRYGEEFKKSM
                     EEGNWDLSKIDYDEVAKSQATLVFGQMNEPPGARSSVALSGLTVAESFRDGVGENKKG
                     NDILFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLSTEMGIMQERITSTKYGSITS
                     VQAVYVPADDLTDPAPATTFSHLDATTVLSRKISELGIFPAVDPLDSTSRILDPLVVG
                     EDHYNTAQRVKQILQRNKELQDIISILGMEELSEEDRITVNRARKVQRFLSQPFYMAT
                     QFSGVPGVMVPIEETIRGFKMILDGEMDEYPEMAFLNVGTIDDAIAKGKKLLDQAKK"
     gene            complement(10307..11116)
                     /gene="nagB"
                     /locus_tag="PKH58_06235"
     CDS             complement(10307..11116)
                     /gene="nagB"
                     /locus_tag="PKH58_06235"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNX88660.1"
                     /translation="MRLIIEPDYQNVSKWAANYIASKILAAQPTQERPFVLGLPTGSS
                     PLGVYKHLIELNKQGVLSFKNVITFNMDEYVGLPQDHDQSYYTFMWSNFFSHIDILPE
                     NVHILNGNAENLEDECAAYEAKMKEVGGIDLFLGGIGPDGHIAFNEPASSLQSRTRQK
                     TLTTDTMIANSRFFDNDVNKVPKTALTVGVGTVLDAKEVLIIVNGYNKARALRHAVEE
                     PINHMWTISALQMHPKGIIVCDYDACAELKVGTYKYFLDIEKDKLDPDTIL"
     gene            11444..11878
                     /gene="ssb"
                     /locus_tag="PKH58_06240"
     CDS             11444..11878
                     /gene="ssb"
                     /locus_tag="PKH58_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNX88661.1"
                     /translation="MSINKAILVGNVGKEPEVRYLEKNVAVANFTLATTERGYTMQNG
                     TQVPDRTEWHNVVAWRNLAEIVEKYVRKGTQVYVEGKIQTRSWEKDGVKRYTTEIYAE
                     TIQLLGKRPEQAEVNGGTVPVAAVQASAEPPVMGAGEDDLPF"
     gene            12188..12709
                     /locus_tag="PKH58_06245"
     CDS             12188..12709
                     /locus_tag="PKH58_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNX88662.1"
                     /translation="MAYIKSVRGFTPVIGKDCFLAENATVVGDVVMGEGCSVWFNAVL
                     RGDVNSIRIGNHVNIQDGSVLHTLYQKSVVEIGDYVSVGHNVTIHGAKIDDYALIGMG
                     AIVLDYAEVGEGAIVAAGALVLSNTKIPPYTLWAGVPAKFVKNVEKEQTNEMNRKIAH
                     NYAMYAGWYKEEL"
     gene            12791..13192
                     /locus_tag="PKH58_06250"
     CDS             12791..13192
                     /locus_tag="PKH58_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88663.1"
                     /translation="MLKEDYLMRLFQLFTECLAKWLSKRKDDDFELIVSFNNEVVEPY
                     LGKDIEFFEKKELDELLVYFQETYPNEQERLSRLEILAELLYQSVLLENDPARRKCLS
                     DKALKVFQHIDKTDKTFSFSRQDRMAELRQF"
     gene            13264..14751
                     /locus_tag="PKH58_06255"
     CDS             13264..14751
                     /locus_tag="PKH58_06255"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HNX88664.1"
                     /translation="MIIIDLIKQDRKKNFRAQGFYKSLSVKLLMGFMGLYFAVIFLSM
                     GYFLGDMLDELDGALKPLELFNGATLYILMGALMLRFFMQSLNTLNLQTYQALPIKRS
                     TLVNFLLLKPVFAIGNYLTLLVVIPFAIRSVAGYYSAATAVTFILNCIMLIWFDLWLA
                     TWLKRRFGSSLMALLVVLVVVGGFVALEYYKVFSLFGVSMQVFNYLSTATFGWLITLL
                     FAALAYGLNLWFFSRNYYPEKFNEKLKLNDSRVTGGFSFLDRFGITGELISLQIRLML
                     RHKRTKTLLYLSAFFLVYGLLFYTNPIYKDQPGWLFFCAMFMTGILMLMYGQWVFSWE
                     SSYFDSILTKNIPVDTYIRSNYYLLVAFNVISFVLTTPYFFFGTKIILLHIAAFLFNT
                     GVNIMLLIFFGTFNTKRIDLNARSSFNYQGTTFKSFLIIMPIMFLPMIVVGVVSSLAS
                     MNAALIVLGVSGLLGIIFKEPLLKLCISQFRKRKYAMANGFREIA"
     gene            14884..>15287
                     /locus_tag="PKH58_06260"
     CDS             14884..>15287
                     /locus_tag="PKH58_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX88665.1"
                     /translation="MIQISNLSKIYSDVTVLNIDSLFVRQGESFGLVGNNGAGKTTLF
                     RLILDLIEPNSGDVSINGEPVARRDEWKSLVGSFLDEGFLIDYLTPDEYFKFVGKLYN
                     KSEGDIAQFLEGMKDFFNDEILGGKKLIRDLS"
BASE COUNT         4219 a         3217 c         3272 g         4579 t
ORIGIN      
        1 acaggtattc tgaattattg tattgaagcc ggagtgaagg gaatactttg ttttggattt
       61 ggaatgaccc tgagggaagg tgacagggaa tattattaca gcaaactgga tgaacatttt
      121 ccgggattga aagaaaaata tcagcataaa tatggaaatg cttatgaaat tacgagcgac
      181 aataatccaa agctgatgaa aatcttcaat gaaacatgct cagcaaatca tatcatgacc
      241 gacgtaaact cgatattcag ttttttgcat gaattcccgg aaaagaatag aaacagtcaa
      301 ttggatctat tcggtcagga atgattttgc aaaacgacag gaaatgattt tgattttgta
      361 gaagttcctc tcctacacac ttgtggagag gagcttttgc atttcaggag catcgcggat
      421 tctttagttt atctatttct ggatgccctt tgtctatttc agttagcttt gagcgaagtt
      481 tatttttatt cgtttattgg cggaaaataa gtatatttgc gttgcaatga acaaataacc
      541 gacttaattc gcatgacagc tttaggaact tttgaactca ataatatata tcagggtgac
      601 tgtgtggaac ttttaaataa aattcctgat aattcggtcg atctgatatt tgccgatccg
      661 ccctacaatc ttcagctcaa tggtgatctg tatcgtcccg atttatcgaa agtagatgcc
      721 gtcgatgatg aatgggataa gtttgattca tttggcgagt atgatcagtt tacaaaccag
      781 tggcttgcgg agtgtcaccg cgtactcaaa cccacaggaa gcatctgggt catcggctcg
      841 tatcacaata ttttcagggt cggaactgcg ctgcagaata tcggtttctg gattctgaac
      901 gatatcatct gggtgaaatc caacccaatg cccaatttta aaggaacccg attcaacaat
      961 gcccatgaaa cgcttatctg ggccacaaaa tccaaatctt cgaaatttac attccattat
     1021 cattccatga aggtgatgaa cgacgacctg cagatgcgca gtgactgggt tattcccatc
     1081 tgtcagggca acgaacggat aaaagtaaac ggaatgaaag ctcattcaac ccaaaagccc
     1141 gaggagttgc tctatcgtgt actgatttcc tcctccaatc cgggtgatgt cgtgcttgat
     1201 ccgttttcgg gaagcggaac tactgctgct gttgccaaac gtctcggcag aaggtacatc
     1261 gccttcgaac gggacgaatt ttacatccgt gtggcccgtg aacgactgga aaaaattacg
     1321 cctgttgacg gaaaaattac gggatacccg atagaaaacc gaattccgaa ggttccgttc
     1381 aacagcctga ttgttgaaga ttacatccgg ctgggggaat tgctctattc gaaagacgga
     1441 aaggtcagtg ccacagtcaa tgctgacggg acacttctcc acgatggctt tgtgggctca
     1501 atacacaaag tgagcgccaa aatccttaac cggtcgaata acaatggctg gagcttctgg
     1561 tatgtaatgc gtgacaataa actggttagt attgacactt tacggtatga atatgaaaag
     1621 aaatatatta aaagaattca acataaattg taaatagcct gcaatgtctt attcgctgat
     1681 taatccaggc gtttatgact gtaaatgttg tcccacccgc tgtgacggta ttacagtgcc
     1741 gaagtttgat tttgggcggg atactgcttt ttccgaagcc gtggaaaatg aggtaatcaa
     1801 ttatattaac cggaattatc cggcactttt tgctgtaaag accagtcagg aaggttatcc
     1861 tgatatcgaa gtgagccgta tcgactcgtc ccaagtctgt ttatatatcg aaattaaagt
     1921 acaatcccgt acgtttatgt ccgtccggaa atacctgcct gatgcaggac tgtatccctc
     1981 cgaaactctg gctcttaacc tgagcgatct ggaaaggtat tttgttgtaa aggaaaatga
     2041 aaaagttcct gtattcatta catggtgcct gttaaaccgt ccctgcgtgg tgggtgttga
     2101 accaggcggc aagtgttttt tttctcagaa tttggatgaa cttcagaaaa taaggttgat
     2161 ggataacaac aattaccgga gatttcgaag ggcatcgggc gatggcgata tggtaaacgg
     2221 agtgcataag ggtgtggtgg ttaattacca cttttctatt cgtgaattgt tcaacggtct
     2281 gcccgatctg aataaattat aagcaaaatg aaacaagcgg cctgcaggtc gcttgtcagt
     2341 tatcttcctg aaatgaattt tggaagaatg ttagttatac gaataatcag atatttcagt
     2401 ttaactttgg ccaaaagaac ctccgacaat atcaagcagt tcgttggtga tggcttgctg
     2461 gcgcgattta ttgtattgca gattaagttc ctgcagcagg tcgtcggcat tgtcggtggc
     2521 tatctgcatg gcaattgtac gtgcggcatg ttccgaagcg ttcgaatcca gcaatgccgt
     2581 atacactttc agtcgcaata ctttaggtat cagatcggtc attatgttaa tcctgtccgg
     2641 ttccactatg taattcagtt gtctgccggt gtttttttca ttactgactt caagggtaat
     2701 aggcaggagg gtttcatcaa ccagaacctg tgaactttta ttgttgaaat gatggtagat
     2761 catctccacc ttatcgatac ttttattaat gaaaagttcc atcagatcgt cagccaggtc
     2821 ctgagtagcg ggataggtag gtttgtcggc cagttcatca aaattatcgg cgaagtaatc
     2881 cattttcttc acgacacggg caattttttt acctaccgga taaatcagga tgttttcttt
     2941 tcccaggtgg ttgtagcgtt ccagtacagt gttaagtctt tttgctacat tggaattgaa
     3001 acctccgcaa aggcttgagt tagatgaaaa aaccagtatg gcaacgcgtt tcacttcacg
     3061 ttgttcggca tagacagagt tgttctcact ttctgcgctg aggaagttgt ttaaaatgtg
     3121 agtcagtttg cgttcataag gataaaaatt ttcaatcctt ttctgtgctt tacgcaattt
     3181 tgcagacgaa accatcttca tcgctgaagt gatcttctgc gttgacttta ccgactgaat
     3241 tcttgatttt atttccttta atgaagccat gattcggact gttagactga tagagccatc
     3301 atttatagag ccagtaataa tgatcagaga acttagatat aaagaaatct gacccgtaat
     3361 taatgaactc caaactcaat acttttctat aaactattaa ctaaaaccgt actttatatt
     3421 cttcttgcaa cttcttcggc cgttttttcc agtatagatg ttacggtgtc gtcaataatt
     3481 ccttttttga ttacatccaa tacatcttcc tggtgtgtaa gttccagtgt actcaggaat
     3541 tccttctcaa attcggaaac tttgtcaagt ggaactttag agagtagacc atgtgtaccg
     3601 caataaagta cagcgacctg tttttctacc gccattggct gatggagcga ctgaaccagc
     3661 agacgggtgt ttttctgtcc cttgtcaatg gtcatggcag tcactgcatc catatcacca
     3721 ccaaatttgg tgaaagcttc cagttcacgg aactgagcct ggtcaatttt cagcgtaccg
     3781 gccactttct tcatggcttt gatttgggca gtaccaccta cacgtgatac cgaaataccg
     3841 acattaatgg cgggacgatt accctgattg aaaaggtcag tatcgaggaa tatctgaccg
     3901 tcggtgatgg aaattacatt cgtaggaata taggctgaca cgtcaccggc ctgtgtctcg
     3961 atgataggca aagctgtaag cgatcctcca ccctttaccc tgtccctaag ggactcggga
     4021 aggtcattca tgcttttggc tacctcttcc tggttgataa ttttagcagc acgttccagc
     4081 agacgggagt gcaggtagaa gatatcgccg ggatatgctt cacgaccaga aggacgacgg
     4141 agaatgagcg aaacttcacg gtaggcaact gcctgtttcg ataagtcatc ataaattaca
     4201 agtgcatgac gtcccgtatc gcggaaatac tcaccaatgg cagcgcccgc gaaaggagcg
     4261 tagtattgca gcgcggcagg atcagatgca ttggcggcaa caacaatggt atattccagt
     4321 gcgttatgtt ctttcagcgt attaacgata ttagcaacag tagagccttt ttgtccgata
     4381 gcaacataaa tgcagtaaac cgggtcacct tcgtcgaaat ttctccgttg attgatgatg
     4441 gtatcgattg cgatgcttgt cttgccggtc tgacggtcgc caatgatcag ttcgcgttgt
     4501 ccgcgtccga taggtatcat ggcatcgatg gccttcaagc ccgtttgaag tggctgtgtt
     4561 accggttgac gatagattac gccaggagct tttcgttcaa gcggcatttc tatgagttca
     4621 ccggtcactt tgccgcgtcc gtcgagtgct tctcccatcg gattaatgac acgacctagc
     4681 ataccttcgc ctacgaaaat ggaagctaca cgtttggtgc gttttacact gtacccttcc
     4741 ttaatttcgc tggttggacc cagcagtaca gctccaacat tgtcttcttc caggttcatc
     4801 acgatagcct taataccgtt atcgaactca agcaattcat tggcttcggc attatgcaat
     4861 ccgtaaatac gtaccacgcc atcgcttacc tgcaatacgg tacctatctc atcaaactta
     4921 acaccggtgt caattccttc gagttgcatc cggagaactt cggacacttc gctcacttta
     4981 atactgtctg acatatctta tcgttaaaat tttacttttt ctgaacaaca actattgtta
     5041 tcgtattttt tttgaatgta actttataag aatcagttac actattcttc tatttctttc
     5101 tataaattca ttttttatcc gggtgagttg tcccgagatg cttgcatccc agcgtacatc
     5161 ctccacttcg agtataaatc ctcccataat ggcaggatcg attacctttt ccagctccag
     5221 agttcctcca acgttattgg taaccaacgc catgaggcgc tcctcagtca cttcatccac
     5281 cgcggaagcg gtagtgagtt taccatagcg gatatttttc tgctttcggt ataagtcgat
     5341 aaaactcaac atgatattcc tgatttgagc ctcacggtta tttttcagca gcagatctgt
     5401 aaatttttca aatgttttgc tggtttttcc gccggctgca ttgagtatca actgacgttt
     5461 ctctgctgtc ggtaatacgg gattgtcaag cacaattgcc agttcactca ttttcataaa
     5521 catgagcgtc aggagtttgg cctcaccata cactttttcc atggagtttg tttctctggc
     5581 aaagtcaagt aaagctgtag catatcgcag tgaaattaat cctgtattca taattttttg
     5641 agtaaacgag tgaacaagtt acatgtttgc atgcaaacgg tttaacaagt aaacaataaa
     5701 ctattatttt atttgcctgt aacaggtaat aaacaacctg ttaaccctgc ctgtgataaa
     5761 caggcattcc gacaatctat gattttggta tgttgatttc gtccagcaga cggtcaatca
     5821 tatccatttg tgaattcttt ttgtccagtc tttcccggag cactttttca gcaatatcca
     5881 ccgaaagcga tgcaacttct ctgcgtatct ggcggattgc atcctctttt tcgagcgcaa
     5941 tttgatgacg cgcgccttcg aggatttttg ccgcttcttc aatggctttt tctttagcgc
     6001 catcaacgat cagatcgcgt gatttggctg cttcggccat aattttcgct tgttccttgc
     6061 gggtttcgtt gattaaggat tcgctgtcgg ctttcactct tgagagctct tcacgggctt
     6121 gtttggcaat cagcagcgat tcgtcgatgt agttcttacg cttttccaca ccgttcagga
     6181 tgatcggaaa accgaatttg acgagcacaa agacaacggc gccgaatgca ataagcatcc
     6241 agaacaacag tcccaaatca ggtgttagta aagacatgtt tatagatgat tagagaatga
     6301 aaccacaaac tactacggca aagagagcta ccccttcaat caaagcggca gagataatca
     6361 tattgatacg gatgtcacct gctgcttcag gctgacgggc gattccttcc atcgctgaac
     6421 taccgatttt tccaatgcct aaacctgctc cgatagctgc aagacctgca ccaactgccg
     6481 aacccaatgt tcctaaaccg gctgcaccgg ctgcctgtaa taataatgaa agtaacataa
     6541 tttttatttt ttaatggttt atatttttat ttattctcag aattaaaata ctgatttgtt
     6601 ttttctatgc aacttctttg tgatgatgtg gctcaatatg tgagagaccg ataaataccg
     6661 ctgaaagcat cgtaaacaca taagcctgaa tgtaagccac cagtaattcg acaaagtcga
     6721 taaaaatggt gaatattacc gaaactacgg tcattgatgc attggtaggg gtgcccagac
     6781 ttacggttac aaatatcaga caaaccagtc ccaatactat ggaatgtccc gccagaatgt
     6841 tggcaaagag acgaatcatg agtgcaaatg gcttggtgaa cactccgatt acttcaatgg
     6901 cgggcataat aggtatcgga accttcagcc acataggtac atccggccag aaaatttcct
     6961 taaagtattc ttttgtaccg gtaatattaa caatgagaaa tgtgcataca gcgagtacaa
     7021 aggtaatggc aatgtttccg gtcacattcg ctccgcccgg aaatataggt atcagtccca
     7081 ataagttgtt ggtgaatata aagaaaaata cagtgagcag gtaaggagca aattttttat
     7141 aatccttacc aacaccgggt ttgatgatgc cgtcgtacac agaaaggata aacatttcca
     7201 tcatgccgac aaatcctttt ttagcttcaa gcggattgcg cttgtacgaa cgggcaatca
     7261 gcaggatgat aacaataaga actaccgcac tgatgagcag cgaagccgca tttttggtca
     7321 gtgatatatc aaccggacga atttcctctc cggttgcatt tttttctaca atttttcctt
     7381 tgtgatcacc ttccttcgca atgtagaagc cgttgtgact ttcgtggccg tgatgaaaag
     7441 cggatgacat aaacaaattc cacccagtgg ttttgctgta aagtatcacc ggcagtggta
     7501 ctgagatgtg tttctccccg atggtggtga tgtgccattc gtaagtatct cccaggtgtt
     7561 ccagtattaa ttcctttacg ttcagttcac cggattcttc tgtttgatgc tcagttgcat
     7621 taactgccga aggttcattc gctctcaaag cagtagcatt aatcagaaga atgactgtga
     7681 gcagtgaaaa tatattaagt agtcttttca ttctgttttt tcagttaagt cgttttttac
     7741 acccgcattt ttcttcatca ttttctctac ctgtaaatac atccaagtct caaatatcag
     7801 gtaaatcata taaaaaacaa taaaaagcag ggcgaaattc ttgatttcgg ccttatttat
     7861 cagccagaaa atcaaaagaa aagccaggct tgtcaaaacc tttatcaccc tgagcagcat
     7921 gtatttattt accagttttt tgctttcagc cgggtttatg ccggtaagca catatatcaa
     7981 tgtcagacca atgatgtaaa agaataccgg tatcaatgtg aatgcagaaa ccttaaattg
     8041 aggcattaca tcttccgtca gatatttcag tccgaaacca gtaatcagaa ttactgccgt
     8101 aagaataagg ataaatttat ttctgctctt ttccattagc ttctaatcaa tagcttccag
     8161 acaaaccgta atcacgttgt tgctcatttc tacaaatccg cccttcaccc tgaactcttt
     8221 actcacacct cctgaaacgt acaccacttt gcccttaccc agtgacgaaa taaacggagc
     8281 gtgattctcc agcagggaaa acgaaccatt tgtgcctggc agtgttacgg atgttacatc
     8341 gcctgaaaaa taaatttctt cgggggttac aatgtcaagt ttcatgttgt tcttgttcct
     8401 ggttcttctt tcttatgtct tcttgtcctg gcacttgtta ttttttcgcc tgatccagca
     8461 gttttttacc tttggcaatt gcatcgtcaa tggttcctac attgaggaaa gccatttcgg
     8521 ggtattcatc catttctccg tcgagtatca ttttgaaacc gcggatagtt tcctcgatgg
     8581 gtaccattac gccgggaaca ccgctaaact gggtggccat ataaaatggc tgtgacagga
     8641 aacgctgtac ttttcgggcg cgattcaccg ttatacggtc ttcttccgat aattcttcca
     8701 tacccaggat ggagataata tcctgcaatt ccttgttacg ctgcaatatc tgttttacgc
     8761 gttgagcggt gttgtaatgg tcttcaccaa ctaccagtgg atccagaata cgtgaagttg
     8821 aatcaagcgg gtcaacagca ggaaagatac ctaattctga aattttacgg ctcaatacag
     8881 tcgtagcatc caggtgagag aatgtggttg caggggctgg gtcggtaagg tcatcagcag
     8941 gcacatacac agcctgtacc gatgtgattg aaccatattt tgtagaagtg atacgttcct
     9001 gcataattcc catctcagtc gagagggttg gttgataacc tactgccgat ggcatacgtc
     9061 cgagcaaagc cgatacttca gaacccgcct gggtaaagcg gaatatattg tcgataaaga
     9121 acagaatatc gttccctttt ttgttttcac caactccgtc gcggaacgat tcggctacgg
     9181 taagtcctga aagtgcaacc gacgaacggg ctccgggagg ttcgttcatc tgtccgaaca
     9241 ccagtgtagc ctgcgatttg gcaacttcat cgtaatcaat tttgctcaaa tcccagttgc
     9301 cttcttccat gcttttttta aattcttcac catagcggat tactcccgat tcaatcattt
     9361 cgcgcagtag gtcgttccct tcacgggtac gttcacctac tccggcaaac accgagaatc
     9421 cactgtgccc ttttgctata ttattgatca gttcctggat aagtacggtt ttacctactc
     9481 cggcaccacc gaacaatccg attttacccc cctttgcgta aggctcaatc aggtcaatca
     9541 cttttatgcc ggtgaaaagt acttcggttg tagtggagag atcttcgaat tttggcggtt
     9601 cgcggtgaat aggatatgca ccttcgcggt cgagctgttt catcccgtcg atagattccc
     9661 ctataacgtt cataagtctt ccttttacct gttctcctac gggcatggtg attggttgtc
     9721 ctttcactat taccggtaaa ccccgtctca gaccatcggt ggagtccatg gctactgtac
     9781 gcaccgtatt ttcaccgatg tgttgctgaa tttcaacaat caattccctt ccgtcaccac
     9841 gattaatgct cagggcgtcg tgtattgccg gtagtttaac ttcctctttt gccgataaat
     9901 caaatgcaac gtccactaca ggaccgatga tttgtgaaat acgacctact aattctgaca
     9961 taatatccgg gtatttttaa ttcatttttc gagattgcaa acgtacaaaa aaacttttat
    10021 tgctacaaat cagaaaggtt tcgtttttag atatcgatgc ctacatatta acataagatt
    10081 aacaaatgag ttcactgttt acagcctgca tttttgagag tattcagttc cgcttattgg
    10141 agcaagtatt cttattttat aaagaatcgt gagttggttt ataccgggat aatattgagc
    10201 cagacttaaa gctattttga catgaagatg acataacgga atgaaggtga gaatagaatt
    10261 caaaaaaaat actgttcagc gatagagaac agtatttttt tgtttcttat aggatagtat
    10321 ccggatcaag tttatctttt tcgatgtcga ggaagtactt gtatgttccc actttcagtt
    10381 cggcacacgc atcgtaatcg catacaatga tacctttcgg atgcatttgc aaggcactga
    10441 tagtccacat atgattgatc ggttcttcca cagcgtgacg cagcgcacgg gctttgttat
    10501 atccgttaac aattatcagc acctctttgg catccagtac agtgcccaca ccaacagtaa
    10561 gggctgtctt cggcacttta ttcacatcgt tatcaaagaa acgtgaattg gcgatcatgg
    10621 tgtcggtggt aagagttttc tgacgggtgc gcgactgaag cgatgaagcc ggttcgttga
    10681 aagcaatgtg tccatccggg ccaattccac ccaggaacag atcaatacct cccacttctt
    10741 tcattttagc ttcataagct gcacattcgt cttccagatt ttctgcgttg ccattgagaa
    10801 tatgtacatt ctcaggcagg atatcgatgt ggctgaagaa attggaccac ataaaagtgt
    10861 agtaactttg atcgtgatcc tgaggtaaac ccacatactc gtccatattg aaggtaatga
    10921 cgtttttgaa cgacagcacc ccctgtttat tcagttcaat caaatgttta tatactccaa
    10981 gaggtgaaga gccggtaggt aagcccagta caaatggtct ttcttgtgta ggttgagccg
    11041 ccagaatttt tgacgcgatg taatttgcag cccatttcga aacgttctga taatcaggtt
    11101 caatgataag tctcatagtt gattttgttt taaatatatg acttacaaaa gtacaatttt
    11161 tattttggtt tgatgcgttg aataactgaa ttattatttt tttattctcc tgaataaagc
    11221 tgttttactg tttatgttgc tggcacttga aaatgaatga tttttttcaa aaaaaaaact
    11281 aaaggttaaa aaaaacatgt atatttgcgt tattgttttt tgaaaaattt ccgtttgttt
    11341 atattcacat tgagaaattg ctgaataata agggctaatt tgtaaaaaaa gcaagacgta
    11401 ttttaactgt aaaacaaaac attaactcta acatttttta attatgtcaa tcaacaaagc
    11461 aattttagta ggaaatgtgg gaaaagaacc cgaagtacgc tatcttgaaa aaaatgtcgc
    11521 ggttgccaat tttacacttg caacaaccga acgcggctac accatgcaga atggtactca
    11581 ggtgcctgac cgtacagaat ggcataacgt tgtagcctgg agaaatctgg ctgaaatcgt
    11641 tgaaaaatat gttcgcaaag gcactcaggt gtatgtggaa ggaaaaattc aaacccgctc
    11701 gtgggaaaaa gacggagtaa aacgttatac aactgaaata tatgccgaaa ccattcagct
    11761 tctgggaaaa cgtcccgaac aggctgaagt taacggtggt actgttcctg ttgcagcagt
    11821 tcaggcatca gcagaaccac ccgtaatggg ggcaggtgag gatgatctcc cgttctgata
    11881 atgcacaata aatctaacta aagtatattt attgaaagaa cacacagtga aacctgtgtg
    11941 ttttatttta atgtgagttc gatgtaaaaa aacatttaac caattcaaat acaattgcct
    12001 gtattcccgt agggaattaa tattcataga aaaaggttat taaattaatt tccccgttag
    12061 gggataaata tatcaattcc atacgggaaa tgaaccaata taaaaacacg ctttctattg
    12121 atatttaagt ccttacggac tatttcttat gtcgaactcc cgttatttta ttataaaaca
    12181 aaaaaatatg gcttacatca aatcggttcg gggttttaca cccgtgatag gaaaagactg
    12241 ctttctggca gagaatgcaa cggtagtggg cgatgtggtg atgggtgaag gatgcagtgt
    12301 gtggttcaat gctgtgctgc gcggtgatgt caattccatc cgcataggta atcatgtgaa
    12361 catccaggat ggctcggtgc ttcacacgtt ataccaaaaa tcggttgttg aaattggaga
    12421 ttatgtttcg gtaggacaca acgtgaccat tcacggagca aaaatagatg attatgcgct
    12481 gataggtatg ggggcaattg tgctcgatta tgcagaagtg ggcgagggag ctattgtagc
    12541 tgccggagca ctggtgctca gcaacaccaa aattccacct tacacccttt gggcaggtgt
    12601 gcccgctaaa tttgtgaaaa acgtagaaaa ggaacaaacc aacgagatga accgcaaaat
    12661 agcgcacaat tatgccatgt atgccggttg gtataaagaa gaattatgaa ttatgaatta
    12721 tgaattatga atgaaaaccg cagctcatcg ctcataattc atcactcata attcatcatt
    12781 ttaaaaaatc atgcttaaag aagattacct gatgcggcta tttcagcttt ttaccgagtg
    12841 tctggctaaa tggctgtcga aacggaaaga tgatgatttc gaattgattg tgagttttaa
    12901 caatgaagtc gtagagcctt atctggggaa ggatattgaa ttttttgaaa agaaagagct
    12961 ggatgaactc ctcgtttatt ttcaggaaac ctatccaaac gagcaggaaa gactatcccg
    13021 gctggaaatc ctggccgaat tgttgtatca aagcgtgttg ttagaaaatg atccggcgcg
    13081 ccggaagtgc ttgtcggata aagcgctgaa agtatttcag cacatcgata agacggataa
    13141 aacgttttcc ttttcccgtc aggatcggat ggcggaattg cggcaattct gagctgccgg
    13201 tgatattcct cgtgttacaa acggttttta ctattttgaa attcagaaat aaataaatta
    13261 tatatgatta ttatcgattt aattaaacag gaccggaaaa agaactttcg ggctcaggga
    13321 ttttataaaa gtctttcggt caagttgctc atgggcttta tgggcttgta ttttgctgtc
    13381 atattcctgt ccatggggta ttttctgggc gatatgctcg acgaacttga cggcgcgctg
    13441 aagcccctgg aactctttaa cggtgcaacc ctttacatcc tgatgggagc actcatgctc
    13501 cgttttttca tgcagtcgct gaatacgctc aacctgcaga cataccaggc attgcccatt
    13561 aaacgttcta cgctggtcaa cttcctgctg ctcaaaccgg tttttgccat cggcaactac
    13621 ctcacactgc ttgtggtcat cccgtttgcc atccggagtg tggcaggcta ttattcggcg
    13681 gctacggctg taacttttat cctcaactgc atcatgctca tctggttcga cctctggctg
    13741 gccacctggc tcaagcgaag gttcggatcg tcgttgatgg ctctgctggt ggtgctggtg
    13801 gtggttggcg gcttcgttgc cctggagtat tataaggtgt tttcgctctt cggagtgtcc
    13861 atgcaggtgt tcaactacct gtcgacagca acattcggct ggctgattac attgctgttt
    13921 gccgcgctgg cttacggact gaacctgtgg tttttctccc gaaattatta tcccgaaaaa
    13981 ttcaacgaga aactgaaact gaacgacagt cgggtgacag gtggattttc cttcctcgat
    14041 cggtttggca ttacgggaga acttatttca ctgcaaataa ggctgatgct ccgacacaaa
    14101 cgaactaaaa cactgttgta cctttcggct ttcttcctgg tttacggact gctgttctat
    14161 accaatccga tatataaaga tcagcccggc tggcttttct tttgtgccat gtttatgacc
    14221 ggcatactca tgctcatgta cggacagtgg gttttcagct gggaaagctc gtatttcgac
    14281 agcatactga ctaaaaacat tccggtggat acctatatcc gctccaacta ttacctgctg
    14341 gtggctttta acgtaatcag ttttgtactt actactccgt attttttctt tggaacaaaa
    14401 attattctgc tgcatattgc cgctttcctg ttcaataccg gtgtcaatat catgctgctg
    14461 atatttttcg gaaccttcaa tacaaaacgg atagacctta acgcccgcag ttcgttcaat
    14521 tatcagggaa cgacgtttaa gagttttctg attatcatgc ccatcatgtt tttacccatg
    14581 atagtggttg gggtcgtttc atcgctggca tccatgaatg ccgccctgat tgtgctggga
    14641 gtatcgggat tgctgggcat aatttttaag gaacctctgt tgaaactttg catcagccag
    14701 ttcaggaaaa gaaaatatgc gatggctaac ggtttcagag agatagcgta agagaagccc
    14761 tctctaactc ccctcctggg ggaggataag acaaaaatac atttagtaaa aacaaacaag
    14821 agataaaaac aaacgctttc tttaagtccc ctaaggggga tttagggggc taaaaatatt
    14881 aatatgatac aaatttcaaa tctttcaaaa atatacagtg acgttacggt acttaacatc
    14941 gactcactgt ttgtacggca gggcgaaagt ttcggcctgg taggaaataa cggtgcggga
    15001 aaaaccacac ttttcagact gatactcgat ttgatagaac ccaattcggg ggatgtaagc
    15061 atcaacggcg aaccggtagc ccgccgggat gaatggaaat cgttggtggg ctctttcctc
    15121 gatgaaggtt tcctcattga ttaccttact ccagatgagt actttaagtt tgtggggaaa
    15181 ctctacaaca aatcggaagg cgatatcgcc cagtttctgg aaggcatgaa ggatttcttc
    15241 aacgatgaaa ttctgggagg caaaaagctg atacgcgacc tctccaa
//
LOCUS       DAOPYB010000059        14906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4345_length_14906_cov_11.906404, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000059 DAOPYB010000000
VERSION     DAOPYB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14906
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4345_length_14906_cov_11.906404"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..337
                     /gene="rpsQ"
                     /locus_tag="PKH58_06265"
     CDS             80..337
                     /gene="rpsQ"
                     /locus_tag="PKH58_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNX88666.1"
                     /translation="METRNLRKVRTGVVSSNKMDKTITVAVKWKEKHPIYGKFVNKTK
                     KYHAHDEKNECNIGDTVSIMETRPMSKMKRWRLNEIIERAK"
     gene            343..708
                     /gene="rplN"
                     /locus_tag="PKH58_06270"
     CDS             343..708
                     /gene="rplN"
                     /locus_tag="PKH58_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNX88667.1"
                     /translation="MIQQESRLTVADNSGAKEALCIRVLGGTGKRYASLGDIIVVAVK
                     NVIPSGEIKKGTVSKAVIVRVKKEVRRPDGSYIRFDDNACVLLNNAGEIRGSRIFGPV
                     ARELRATNMKIISLAPEVL"
     gene            728..1039
                     /gene="rplX"
                     /locus_tag="PKH58_06275"
     CDS             728..1039
                     /gene="rplX"
                     /locus_tag="PKH58_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNX88668.1"
                     /translation="MSKLHIKKGDTVYVNAGEDKGKSGRVLEVLVKEQRAIVEGINLV
                     SKHTKPNAKAPQGGIVKKEAAIHISNLNPTEKGKPVRVGRKLNADGKLVRISKKSGEE
                     I"
     gene            1042..1587
                     /gene="rplE"
                     /locus_tag="PKH58_06280"
     CDS             1042..1587
                     /gene="rplE"
                     /locus_tag="PKH58_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNX88669.1"
                     /translation="MNTASLRQDYKERIVPILMKEFGYSSVMQAPKLEKIVLNQGLGM
                     AVADKKIIEVALNEMTTITGQKAVSTISKKDISNFKLRKKMPIGVRVTLRGNNMYEFL
                     ERLVRVALPRIRDFKGIENKLDGRGNYTLGITEQIIFPEINIDSISRLLGMNITFVTT
                     ANTDEEGYALLKEFGLPFKKN"
     gene            1603..1872
                     /gene="rpsN"
                     /locus_tag="PKH58_06285"
     CDS             1603..1872
                     /gene="rpsN"
                     /locus_tag="PKH58_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNX88670.1"
                     /translation="MAKESMKAREVKRAKLIAKYAEKRTKFIEEGNYDLLQTLPRNSS
                     PIRYHNRCKLTGRPKGYMRQFGISRIQFREMASKGLIPGVRKASW"
     gene            1943..2338
                     /gene="rpsH"
                     /locus_tag="PKH58_06290"
     CDS             1943..2338
                     /gene="rpsH"
                     /locus_tag="PKH58_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNX88671.1"
                     /translation="MTDPIADYLTRLRNAIKANHRVVEVPASNLKKEMTRILHEKGYI
                     LNYKFVDDGAQGTIKIALKYDPVNKVNSIKNLKRVSTPGLRQYVGYKDMPRVLNGLGI
                     AILSTSKGLMTNKEAVDLKVGGEVLCYIY"
     gene            2358..2909
                     /gene="rplF"
                     /locus_tag="PKH58_06295"
     CDS             2358..2909
                     /gene="rplF"
                     /locus_tag="PKH58_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNX88672.1"
                     /translation="MSRIGKLPITIPAGVTVTVKDQDITVKGPKGVLVQSVNPNITVE
                     VEGNECKVSRTNDEKQNRAFHGLYRSLINNMVTGVSEGYKKTLELVGVGYRASNQGQV
                     LELSLGYTHTIYLVLPEEIKIETKSERNQNPVIMLESTDKQLLGQVCAKIRSFRMPEP
                     YKGKGVKFKGEIIRRKAGKSAGK"
     gene            2931..3275
                     /gene="rplR"
                     /locus_tag="PKH58_06300"
     CDS             2931..3275
                     /gene="rplR"
                     /locus_tag="PKH58_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNX88673.1"
                     /translation="MSEKSNRRIRIKAHIRTKISGTAEKPRLSVFRSNTQIYAQIIDD
                     VKGVTLAAASSLGSKDKITKTEQAAVVGKLIAKNALEAGIKEVVFDRNGYLYHGRVKQ
                     LADAAREGGLKF"
     gene            3293..3799
                     /gene="rpsE"
                     /locus_tag="PKH58_06305"
     CDS             3293..3799
                     /gene="rpsE"
                     /locus_tag="PKH58_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNX88674.1"
                     /translation="MNKVKTTNDLELKDRLVAVNRVTKVTKGGRAFSFSAIVVVGNED
                     GIVGWGLGKAGEVTAAIAKGTEAAKKNLIKVPVLNGTIPHEQIAKFGGARVYIEPATN
                     GTGVKAGGAMRAVLESVGITDVLAKSKGSSNPHNLVKATMLALSELRDAHTVAQNRGV
                     SLNTVFNG"
     gene            3813..3989
                     /gene="rpmD"
                     /locus_tag="PKH58_06310"
     CDS             3813..3989
                     /gene="rpmD"
                     /locus_tag="PKH58_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNX88675.1"
                     /translation="MATIKVKQIRSRIRCPKDQKRTLDALGLKKMNAVVEHEATPQIL
                     GMVAKIPHLVEVIK"
     gene            4029..4475
                     /gene="rplO"
                     /locus_tag="PKH58_06315"
     CDS             4029..4475
                     /gene="rplO"
                     /locus_tag="PKH58_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNX88676.1"
                     /translation="MNLSNLTPASGSVKTRKRIGRGSGSGLGGTSTRGHKGQKSRSGY
                     SRKVGFEGGQMPIQRRLPKFGFKNINRVEYKAINLETLQVIADSKNLTKIGLQDLREA
                     GYISKTALVKVLGKGKLTSKLEVEANAFSKSAEDAIVAAGGNAVKI"
     gene            4480..5820
                     /gene="secY"
                     /locus_tag="PKH58_06320"
     CDS             4480..5820
                     /gene="secY"
                     /locus_tag="PKH58_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNX88677.1"
                     /translation="MKKLFETLQNIWRIEDLRNRLTTTLLFVLVYRFGSYIVIPGVDA
                     TALAALKHQTSGGIMALLDMFSGGAFQNASIFALGIMPYISASIVIQLLTIAVPKFQK
                     LQHEGESGRTKMNQYTRYLTVLILALQGPSYMANLSVQVRQAGSVLPSSFVFIATSTI
                     ILMAGSMFVMWLGERITDKGIGNGISFIIMIGIISRLPGAIIQEFSSRLFSGGGGLIM
                     FLFEIVVLILVIAFAILLVQGTRKIPVQYAKRVVGNKQYGGVRQYIPLKVNAAGVMPI
                     IFAQAIMFIPIAIAGYSSSESVQGFFHSFTRYTSFWYNFIFGILIIAFTYFYTAITIN
                     PTQMAEDMKRNNGFIPGIKPGKRTAEYLDLIMSRITLPGSILLAIIAILPAFASMIGV
                     SQQFAQFFGGTSLLILVGVVLDTLQQVESHLLMRHYDGLLKSGRIKGRTGASAY"
     gene            5833..6618
                     /gene="map"
                     /locus_tag="PKH58_06325"
     CDS             5833..6618
                     /gene="map"
                     /locus_tag="PKH58_06325"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNX88678.1"
                     /translation="MIFLKTDEEIELLRHCNQIVAKTLAETAKLIKPGVTTLELDKRA
                     EEFIRDNGGVPNFLGYSGYPNSICASVNEQVVHGIPSAKTILKEGDIISIDCGVLKNG
                     FNGDSAYTFCVGEVDPEIVNLLRTTKESLYKGIEQACEGKRLGDIGNAIQTYCEDRGY
                     SVVREMVGHGVGRKLHEAPEIPNYGKKGNGIMLKSGMTIAIEPMINLGSRNLVFEKDG
                     WTTRTADRKPSAHFEHSVAVRHGKADILSSFEYIEQVLGEREF"
     gene            6623..6841
                     /gene="infA"
                     /locus_tag="PKH58_06330"
     CDS             6623..6841
                     /gene="infA"
                     /locus_tag="PKH58_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNX88679.1"
                     /translation="MAKQTAIEQDGTIIEALSNAMFRVELENGHVITAHISGKMRMHY
                     IKILPGDKVKVEMSPYDLSKGRISFRYK"
     gene            6854..6970
                     /gene="rpmJ"
                     /locus_tag="PKH58_06335"
     CDS             6854..6970
                     /gene="rpmJ"
                     /locus_tag="PKH58_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNX88680.1"
                     /translation="MKVRASIKRRTPDCKIVRRFGRLYVINKKNPKYKQRQG"
     gene            6998..7378
                     /gene="rpsM"
                     /locus_tag="PKH58_06340"
     CDS             6998..7378
                     /gene="rpsM"
                     /locus_tag="PKH58_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNX88681.1"
                     /translation="MAIRIVGVDLPQNKRGEVGLTYVYGIGRSSAKKILEEAGVDINI
                     KVKDWTDDQAAKIREIIGASYKVEGDLRSEVQLNIKRLMDIGCYRGIRHRIGLPLRGQ
                     STKNNARTRKGKKKTVANKKKATK"
     gene            7432..7821
                     /gene="rpsK"
                     /locus_tag="PKH58_06345"
     CDS             7432..7821
                     /gene="rpsK"
                     /locus_tag="PKH58_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNX88682.1"
                     /translation="MAKKTVAAKKRVVKVDGVGQLHVHSSFNNIIVTLANSEGQVISW
                     SSAGKMGFRGSKKNTPYAAQMAAQDCSKVAFDLGLRKVKAYVKGPGNGRESAIRTVHG
                     AGIEVTEIVDVTPLPHNGCRPPKRRRV"
     gene            7961..8566
                     /gene="rpsD"
                     /locus_tag="PKH58_06350"
     CDS             7961..8566
                     /gene="rpsD"
                     /locus_tag="PKH58_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNX88683.1"
                     /translation="MARYTGPKTKIARKFGEPIFGADKVLAKKNYPPGQHGNSRRRKT
                     SEYGIQLREKQKAKYTYGLLEKQFRILYEKAKRTKGITGVILMQLLESRLDNIVYRLG
                     FAPTRAAARQLVSHKHITIDGKVVNIPSYHVRPGQVVAVREKNKSMEVILDALSGFNH
                     SKYPWIEYSDSSMSGVYLHIPEREDIPENIKEQLIVELYSK"
     gene            8578..9570
                     /locus_tag="PKH58_06355"
     CDS             8578..9570
                     /locus_tag="PKH58_06355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNX88684.1"
                     /translation="MAILAFQKPEKVIMLEADDFQGKFEFRPLEPGYGITIGNALRRI
                     LLSSLEGFAITSIRIDGIDHEYSTIPGVMDDVTNIILNLKQVRFKQIVEEIENEKVTV
                     SVKGTTTFKAGDLGRYLTGFEVLNPDLVICTLDPSASFQIDITINKGRGYVPSEENKS
                     PNAEIGVIPIDAIYTPIRNVKYTIENYRVEQVTDYEKLIIEVSTDGSIHPKEALKEAA
                     KVLIHHFMLFSDEKISLEVPEGEGVEEFDEEVLHMRQLLKTKLNDLELSVRALNCLKA
                     ADVTTLGELVSNHRHDLLKFRNFGKKSLTELEEKLESLNLNFGMDVSKYKLDKE"
     gene            9577..10101
                     /gene="rplQ"
                     /locus_tag="PKH58_06360"
     CDS             9577..10101
                     /gene="rplQ"
                     /locus_tag="PKH58_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNX88685.1"
                     /translation="MRHNKKFNHLGRTSSHRKAMLANMAVSLIHHKRIATTLAKAKAL
                     RVYVEPLLTKSKEDSTNSRRVVFSYLKNKEAVTELFRDISVKIADRPGGYTRILRTGF
                     RLGDNAEMCIIELVDYNENMLKEKTAKKSTRTRRSSKKADVAVAAEDKAKAPKAAPKV
                     EETKAEATVEEKGE"
     gene            10413..11120
                     /gene="pyrH"
                     /locus_tag="PKH58_06365"
     CDS             10413..11120
                     /gene="pyrH"
                     /locus_tag="PKH58_06365"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNX88686.1"
                     /translation="MIKYERILLKLSGESLAADQKQGIDIVRLEQYAAQIKEITELGV
                     QVGIVIGGGNIFRGLSGASKGFDRVQGDQMGMLATVINSLALNSALQAAKTKSRVLTS
                     VRMEPIGEFYSKRYAVECLERGEVVIIAGGTSNPYFTTDTASSLRAIEIEADIMLKGT
                     RVDGVYTADPEKDKLAVKFEKISYDEIYNRNLKVMDLTATTMCKENKMPIYVFDMDTV
                     GNLKKVILGENIGTLVY"
     gene            11153..11713
                     /gene="frr"
                     /locus_tag="PKH58_06370"
     CDS             11153..11713
                     /gene="frr"
                     /locus_tag="PKH58_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNX88687.1"
                     /translation="MVDYKTHIGDAEELMQASVEYLDEALAHIRAGKANTRILDGVKV
                     EYYGSLVPLSNVATVTTPDAKTIAIQPWEKSLISVIEKAILNSSVGITPMNNGETIRL
                     GIPPLTEERRKQLVKQCRQEGEEAKISVRNARREAIENVKKLVKEGLPEDMEKDAEDE
                     VQKIHDKYVKKIDELLAAKEKEIMTV"
     gene            11804..12727
                     /gene="rsgA"
                     /locus_tag="PKH58_06375"
     CDS             11804..12727
                     /gene="rsgA"
                     /locus_tag="PKH58_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNX88688.1"
                     /translation="MQGLVVKNTGSWYTVRTDEGEIIDCKIKGNFRIKDIRSTNPIAV
                     GDRVKLTLNTDGTAMIHEICSRKNYIIRRSSNLSKYSQILAANLDLVALVITVNYPET
                     STVFIDRFLATAEAYQVPACLVINKTDRYSEDESAFAAALKNLYETINYPVFLVSAIT
                     GDSVPELKEYLSNKTTLLSGNSGVGKSTLINMIAPNSDVRTNKISEYHNKGMHTTTFS
                     EMFLLDDGGFIIDTPGIKGFGTIDMQENEVGHFFREIFRISKECHFNNCTHTHEPGCA
                     VFDAVEKHLISQSRYQSYLSIMEDCKSGKYR"
     gene            12861..13364
                     /locus_tag="PKH58_06380"
     CDS             12861..13364
                     /locus_tag="PKH58_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNX88689.1"
                     /translation="MGHHQIDRLDKKILQLISKDARIPFLEVARECNVSGAAIHQRIQ
                     KLRNLGIIKGSEFVVDTYKIGYQTCAYIGIVLTDIKKFDSVVSELKKIPEVVECHYAT
                     GKYSMFIKIYAKDNRHLLQIILEHLTLIDGVGHTETFQLSLDEIFKRQLSVFEVGEDD
                     LDDIEDE"
     gene            complement(13467..13958)
                     /locus_tag="PKH58_06385"
     CDS             complement(13467..13958)
                     /locus_tag="PKH58_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNX88690.1"
                     /translation="MEWYKFPVILILFILAIGSYSQEIGKASYYGNNLHGRKTANGKP
                     YHRDSLTCAHRSYPFGTLLKIQNLNNGKEVIVKVTDRGPFVRGRIVDLSYAAAKQIGL
                     IGHGTVRVEVTKFDPLKTIEEFYNMDSLGLIKFKTFPDSIFWPIKSTTPQSDYLPVAP
                     KKP"
     gene            14303..14812
                     /gene="infC"
                     /locus_tag="PKH58_06390"
     CDS             14303..14812
                     /gene="infC"
                     /locus_tag="PKH58_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNX88691.1"
                     /translation="MPNRINEKIKGVKEVRLVGDNVEQGIVSIDEALRIAEELELDLV
                     EISPSAVPPVCKIVDYQKFLYQQKKREKEQKAKAAKVVLKEIRFGPQTDEHDYNFKLK
                     HAISFLKEGCKVKAFVFFKGRSILFKEQGEVLLLRFANDLEDYAKVDQLPQLEGKRMI
                     IFLSPKKTK"
     gene            14846..>14906
                     /locus_tag="PKH58_06395"
     CDS             14846..>14906
                     /locus_tag="PKH58_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HNX88692.1"
                     /translation="MPKMKTNSGAKKRFALTGTG"
BASE COUNT         5094 a         2535 c         3128 g         4149 t
ORIGIN      
        1 aagacgtacg tcgtactatt gcccgttttg caactgaatt acgtcagaga gaaataaata
       61 aaaaataatt tagattctga tggaaacaag aaatttaaga aaagtaagaa ctggtgtggt
      121 ttccagcaat aaaatggata aaaccatcac agtagctgta aagtggaaag aaaaacaccc
      181 gatttacggc aaattcgtga ataaaacgaa aaaatatcat gctcacgacg aaaagaacga
      241 atgcaacatt ggtgacactg taagcatcat ggaaacccgt ccgatgagta agatgaaaag
      301 atggagacta aacgaaataa tcgaaagagc taagtaagaa ttatgataca acaagaatca
      361 agactcacag tagccgataa cagtggtgca aaagaagcac tctgtattcg agtgttaggt
      421 ggtaccggaa aacgttacgc atcacttggc gatattatcg tagttgcagt taaaaatgta
      481 ataccttcgg gagaaataaa aaaaggcaca gtatcaaaag ctgtgatcgt acgcgttaaa
      541 aaagaagtac gccgccccga tggctcttac attcgtttcg atgataacgc ctgcgtgttg
      601 ttgaacaatg caggtgaaat tcgtggaagc cgtatatttg gtccggtagc ccgtgaatta
      661 cgtgcaacca atatgaaaat tatatcactt gcaccagagg tgctttaatc aatgaaataa
      721 taaagtgatg agtaaattac acataaaaaa gggagataca gtatacgtaa atgccggtga
      781 agacaaaggt aagtccggac gtgtactcga agttcttgtg aaagaacaac gcgctatcgt
      841 agaaggtatc aacttggtat caaaacatac caaacccaac gcgaaagctc ctcagggagg
      901 catagtgaaa aaagaagctg ctattcatat ttcaaattta aacccgacag agaagggaaa
      961 acccgtacgt gttggacgca aattgaatgc agatggcaaa ctggtacgta tttctaaaaa
     1021 atcaggagag gaaatttaag tatgaacaca gcaagtttaa gacaagatta taaagaacgt
     1081 atcgttccta tcttaatgaa ggaatttgga tattcttctg taatgcaagc gcccaaactg
     1141 gaaaaaatcg ttttgaatca aggcttggga atggctgttg ctgataaaaa aatcatcgaa
     1201 gtagcattga atgaaatgac tacgatcact ggtcagaaag cagtttcaac catttcgaag
     1261 aaagatattt cgaatttcaa attgcgtaaa aaaatgccaa tcggtgttcg ggttacatta
     1321 cgtggaaaca atatgtacga atttctggaa agactggttc gtgtggcttt acctcgtatc
     1381 cgtgacttca agggaattga aaacaaactc gatggacgtg gaaattatac actgggtatc
     1441 accgagcaaa ttattttccc cgaaatcaac attgacagca tctctcgttt gctgggtatg
     1501 aatattacat tcgtaaccac cgccaatacc gatgaagaag gttatgcttt gttgaaggaa
     1561 tttggattac catttaagaa aaactaatat taagtaaata caatggcaaa agaatcaatg
     1621 aaagcccgtg aggtaaagag agccaaactg attgctaaat atgcagaaaa gcgtacgaaa
     1681 tttatcgaag aaggcaacta tgatttgttg cagacacttc cgagaaattc atcgccaatc
     1741 cgttaccaca atcgttgtaa actcactggc cgtcccaaag gatatatgcg tcaatttggt
     1801 atttcgcgta ttcaattccg cgaaatggca tccaaaggct taatcccggg agtaagaaaa
     1861 gcaagctggt aatcagctat atatgcaaag atttattaat taaatattgt ccctgagtag
     1921 ggatcaattt aaaaacaaaa atatgacaga tccaattgca gattatttaa ctcgattgag
     1981 gaatgctatt aaagccaacc accgtgtagt ggaagttcct gcatcgaact taaaaaaaga
     2041 aatgacccgg atattgcacg aaaaaggcta tatcttaaac tacaaatttg tagatgacgg
     2101 ggcacaaggc acaattaaaa ttgccttgaa gtacgatccg gttaacaaag ttaactcgat
     2161 caagaattta aaaagagtat caacaccagg tcttcgtcaa tacgtaggtt acaaagatat
     2221 gccacgtgta ttgaacggat tgggtattgc cattctttct acctccaaag gtttgatgac
     2281 taataaggaa gctgttgacc tcaaagtggg cggcgaagtg ttgtgttaca tttattaatt
     2341 cataggagga aataagaatg tcaagaatag gaaaattgcc cataacaatt cccgcaggtg
     2401 ttactgtgac tgtgaaagac caggatatta ctgttaaagg tccgaaagga gtattagttc
     2461 aatcagtaaa cccaaacatc actgttgaag tagaaggaaa cgaatgtaaa gtttcacgta
     2521 ccaatgacga aaagcaaaat cgtgcatttc atggtttgta tcgttcatta attaataaca
     2581 tggtaactgg tgtttccgaa ggttataaaa aaacacttga actggtcggt gtaggatatc
     2641 gtgcatctaa tcagggtcag gtgttggaac tttcattagg ctacactcat actatctatt
     2701 tagttttacc cgaagaaatt aagattgaaa cgaagagcga acgtaaccag aaccctgtaa
     2761 tcatgctcga aagcaccgat aaacagttac ttggtcaggt ttgtgccaaa attcgttcat
     2821 tccgtatgcc agaaccttac aaaggtaaag gtgtgaaatt caaaggtgaa ataattcgtc
     2881 gtaaagctgg taaatcagct ggtaaataat ctacgtaaac ttgtgaaaca atgagtgaaa
     2941 aatcaaatag aagaatcaga ataaaagccc atatccgtac aaaaatttcg ggtacagctg
     3001 aaaaaccgcg tttgagtgta tttcgcagta atactcaaat ttacgctcag atcattgacg
     3061 acgtaaaggg agttaccctc gcagcggctt catcgcttgg tagcaaagat aaaattacta
     3121 aaacagagca ggctgcagta gttggtaaac tgattgcaaa aaatgctttg gaagcaggaa
     3181 ttaaagaagt ggtatttgac cgtaacggtt acctttatca cggacgtgtt aaacaattag
     3241 ctgacgcagc tcgcgaaggt ggacttaaat tttaagcaca atgacaacaa aaatgaataa
     3301 agttaaaact acaaatgatt tagaattgaa agacagactt gtagctgtaa accgtgtgac
     3361 aaaagttaca aaagggggac gtgcgttcag tttttcagca atcgtagtgg taggtaatga
     3421 agatggtatt gttggctggg gtttaggtaa agccggtgaa gtaaccgcag caattgcaaa
     3481 aggaactgaa gcagcaaaaa agaacctcat taaagtacct gttctgaatg gaactattcc
     3541 acacgaacaa attgcgaaat ttggaggagc aagagtttat atcgaacctg caactaacgg
     3601 taccggagta aaagccggtg gtgctatgcg tgctgtattg gaaagcgttg gtattactga
     3661 tgtacttgct aagtcaaaag gttcatcaaa tcctcacaat cttgttaaag caactatgct
     3721 tgctttatca gaattgcgtg atgcacatac agtcgctcaa aacagaggcg tatcgcttaa
     3781 cacagtattt aacggataaa atcagaatca ttatggcaac tataaaagta aaacaaatca
     3841 gaagcagaat cagatgcccg aaagatcaga aaagaacttt agatgcactt ggtttgaaga
     3901 aaatgaatgc cgtagttgag catgaagcta ctccacaaat tctgggaatg gtagcgaaaa
     3961 ttcctcactt ggttgaagta attaaataag taataatcgt cattcagact taaaaatatt
     4021 aagataacat gaatttaagc aatttaacac ctgcatcagg atccgtaaag actcgtaaaa
     4081 gaattggtcg cggatctggt tccggtttag gcggcacatc taccagagga cataagggac
     4141 aaaaatcacg ttccggttat tcccgtaagg ttggttttga aggtggacag atgcctattc
     4201 aaagacgttt accaaaattt ggtttcaaaa atattaaccg tgtagaatac aaagcaatta
     4261 atcttgaaac tcttcaggtt atagcagata gtaaaaacct gaccaaaatt ggattacagg
     4321 atttacgcga agcagggtat atttcaaaaa cagctttggt taaagtactc ggtaaaggta
     4381 aattaacttc aaaattagaa gtagaagcaa atgctttctc taaatcagca gaagatgcaa
     4441 tcgttgctgc tggtggtaat gcagttaaaa tctaagcgca tgaaaaagtt atttgaaact
     4501 ttacaaaata tctggagaat tgaagatctt cgaaaccgat tgactacaac attattattt
     4561 gtgttggttt atcgtttcgg atcttacatt gtgatcccgg gtgttgatgc cacagctctt
     4621 gctgccttga aacatcagac aagtggtggt attatggcat tgctggatat gttctcaggt
     4681 ggcgcatttc aaaatgcttc catattcgct ttaggtatca tgccctatat ctctgcatct
     4741 atcgttattc agttgttgac aattgctgta ccaaaatttc agaaattgca gcacgaaggc
     4801 gaaagtggac gtaccaaaat gaaccaatac acacgttacc ttactgttct tattcttgca
     4861 ttgcagggac catcttatat ggctaatctc agtgtacagg ttcgtcaggc aggttctgta
     4921 ttgccaagta gctttgtatt catcgctacc tctaccataa ttcttatggc tggtagtatg
     4981 tttgtaatgt ggcttggtga aagaattaca gataaaggta ttggaaacgg gatatccttc
     5041 atcattatga ttggtataat ttcacgtttg ccgggagcta taattcagga attctcatca
     5101 cgcctattct ctggtggtgg tggtcttatc atgttcctgt tcgaaattgt agtgttaata
     5161 cttgttattg catttgcaat tttacttgtt cagggtacac gtaaaattcc tgtacaatat
     5221 gccaaaagag ttgttggaaa caaacaatat ggtggagtac gtcaatatat acctttgaag
     5281 gttaatgctg ccggtgtaat gccaatcatt tttgctcagg caattatgtt tattccaata
     5341 gcaatcgcgg ggtattcaag ttctgaaagt gtacaaggct ttttccattc atttacgagg
     5401 tacaccagtt tctggtataa tttcatattt ggaatactaa tcattgcatt tacctatttt
     5461 tatactgcaa taacgattaa ccctacacag atggctgaag atatgaaacg taataacggt
     5521 ttcatacccg gaattaaacc cggaaaacgt acagcagaat accttgactt aatcatgtca
     5581 cgtattacat tgcctggatc catactatta gcaataatag ccattcttcc agcatttgca
     5641 tctatgattg gcgtaagtca gcaatttgcc caatttttcg gtggaacttc actgttgatt
     5701 cttgttggag ttgtactgga tacgttacaa caggtagaaa gccatttgtt aatgcgtcat
     5761 tacgacggat tactcaaatc aggtcgaatt aaaggacgta caggtgcgtc agcatattaa
     5821 aaatttagat taatgatatt tcttaagact gatgaagaaa tagaattact gagacattgc
     5881 aatcagattg ttgccaaaac gttggcagaa acagcaaagt tgatcaaacc gggtgttaca
     5941 accctggagt tggataaaag ggcagaagag tttatcaggg ataatggtgg agtacctaat
     6001 tttctcggat attccggata cccgaattct atttgtgcat cagtaaatga acaggtcgta
     6061 catggtatcc cttcagccaa aacaatcttg aaggaaggtg atattatatc tatagattgt
     6121 ggtgtactta aaaacggatt taacggagac tctgcctaca ctttttgtgt aggtgaagtc
     6181 gatccggaaa ttgtgaattt attgcgtacc acaaaagaat ctctttacaa gggtattgaa
     6241 caagcctgtg agggtaaaag actgggcgat attggaaatg caattcaaac gtattgtgaa
     6301 gacagaggtt actcagtagt ccgcgaaatg gtcggacacg gagtgggccg taagcttcat
     6361 gaagcacccg agatcccgaa ttacggtaaa aaaggtaatg gtataatgct taaatcagga
     6421 atgaccatag ccattgaacc gatgattaat cttggaagtc gtaaccttgt gtttgaaaag
     6481 gacggatgga caacacgcac agccgatcgt aagccatctg ctcattttga acattcagtg
     6541 gcagtcagac acggtaaagc agatatactt tcaagttttg aatatattga acaagtttta
     6601 ggagaaaggg aattttaatt ttatggcaaa acaaacagca atagagcaag acggaacgat
     6661 aattgaagca ttatcaaatg caatgtttcg ggtagaactg gaaaacggtc acgtgattac
     6721 tgctcatata tccggaaaaa tgcgtatgca ttatattaag attctgccag gcgataaagt
     6781 taaagttgaa atgtcaccct atgacttgtc aaaaggaaga atttcattca gatacaaata
     6841 aaaaacatat catatgaaag taagagcatc aattaaaaga cgcactcccg attgcaaaat
     6901 cgtaaggagg tttgggcgtc tctatgttat caacaaaaaa aatcctaagt ataaacaacg
     6961 tcaaggataa ttatttattt ttgcaaaaaa ttagtttatg gctataagaa ttgtcggagt
     7021 agatttaccc caaaataaaa gaggggaagt tggattgact tatgtctacg gaataggtcg
     7081 cagtagtgca aaaaagattt tagaagaagc cggtgttgac attaacatca aagtcaaaga
     7141 ttggactgac gaccaagcag ctaaaatccg cgaaatcatc ggagcaagtt acaaagtaga
     7201 aggtgacttg cgttcagaag tacaattgaa tatcaagcga ttgatggata ttggatgtta
     7261 ccgtggtatc cgccaccgta tcggtctgcc gttgagaggt cagagtacga agaataacgc
     7321 tcgtacacgt aaaggtaaaa agaaaacagt tgcaaacaag aaaaaagcaa ccaagtaaga
     7381 ggtaattaac tgtttgcgaa ttttagaaaa gcaaaaaaat taaaaaaaag tatggcaaag
     7441 aaaacagttg cagccaaaaa gcgagttgta aaagtggatg gagtaggtca attgcacgta
     7501 cattcatcat tcaacaacat tattgtaacc cttgcaaatt ctgaaggtca ggttatttcc
     7561 tggtcatcgg ctggtaaaat gggtttccgc ggctcaaaga aaaacactcc ttatgctgct
     7621 caaatggctg ctcaggattg ctcaaaagta gcattcgatt tgggattgcg taaagtgaaa
     7681 gcttacgtta aaggtccggg taacggacgt gaatcagcta tccgtaccgt acatggtgct
     7741 ggtattgaag taacagaaat tgttgacgtt actccacttc cacacaacgg ttgtcgtcct
     7801 ccaaaacgta gaagagtgta atatatctgg tttcactgtt tattgatagc agtatttgtg
     7861 gtatgactgc atttcaaacc tctctgcagt cgcggctgcc agaaatccgg agtcaagaaa
     7921 taagtgaaac gaaatcaaaa caaaaattaa attataaaaa atggcaagat atactggacc
     7981 aaaaacaaaa attgcccgta agtttggtga accaattttc ggagctgaca aagtgttggc
     8041 taagaagaat taccctcccg gacaacacgg aaattcacgt cgtagaaaaa cttcagaata
     8101 cggtattcaa ttgcgtgaaa aacaaaaagc aaaatacacg tatggtttgc tggaaaaaca
     8161 attccgtatt ctttatgaaa aagcaaaacg tacgaaaggt atcactggtg ttatcctgat
     8221 gcagttactc gaatcacgtc tggacaacat tgtttatcgg ttgggatttg ctcctacacg
     8281 tgcagcagca cgtcagttgg taagtcacaa acatattact attgatggaa aagtggtaaa
     8341 tatcccctct tatcatgttc gtcccggaca agtagtagct gttcgtgaaa aaaacaaatc
     8401 aatggaagtc attctggacg ctttgagtgg ttttaatcac agcaaatatc catggattga
     8461 atactccgat tcttcgatgt cgggagttta tctgcacatt cccgaacggg aagacattcc
     8521 tgaaaatatt aaagaacaat taatcgtaga gttgtactct aaataatacg tgaagctatg
     8581 gcaattttag catttcaaaa acccgaaaag gttatcatgt tggaagctga cgactttcag
     8641 ggaaaatttg aatttcgtcc gttggaaccg ggttatggta ttaccattgg taatgcttta
     8701 cgccgcatac ttctttcgtc gctcgaaggc ttcgctatta cttcaataag aattgatggt
     8761 atagatcacg aatattcaac tattccgggt gttatggatg atgttacaaa catcattctg
     8821 aacctgaaac aagtacgttt caaacagata gttgaagaaa tcgaaaatga aaaagtaact
     8881 gtttctgtta aaggtactac aactttcaaa gctggtgacc tgggcagata tctgaccgga
     8941 tttgaggtgc tgaatcctga tttggtcatt tgtactcttg atccttcagc cagtttccag
     9001 attgatatta caattaataa gggacggggt tatgtgcctt ctgaagaaaa taaatcaccg
     9061 aatgctgaga ttggtgtaat tcctattgat gcaatttata ctcctatccg taatgtcaaa
     9121 tatacgattg aaaattatcg tgtagaacag gttactgatt atgaaaaatt gatcatcgaa
     9181 gtttcgacag acgggtctat tcatccgaaa gaagctttga aggaagctgc aaaagtttta
     9241 attcaccact tcatgttatt ctccgatgaa aagatatcgc tggaggtacc tgaaggcgaa
     9301 ggtgttgaag aatttgatga agaagtactt catatgcgtc agttacttaa aaccaaactg
     9361 aatgatcttg aactttcggt acgtgcactc aactgtctga aagcagctga tgtaacaact
     9421 ttgggtgaac tggtcagcaa tcatcgtcat gatcttctga aattccgcaa ttttggtaaa
     9481 aagtcactga cggaattaga agaaaaatta gaaagcttaa atctgaactt cggaatggat
     9541 gtgagcaaat ataaattaga taaagagtaa gtaaaaatga gacataataa aaaattcaat
     9601 catttaggcc gtacgtcttc acacagaaaa gctatgttgg caaacatggc ggtttcttta
     9661 attcatcaca aaagaattgc gactactctg gcaaaagcta aagcactacg tgtttatgtt
     9721 gaaccacttc tgaccaaatc caaagaagac tcaactaatt cgcgccgtgt ggttttcagc
     9781 tatttgaaaa ataaagaagc tgttacagag ttattccgcg atatctcagt aaaaattgca
     9841 gatcgtccgg gtggctatac acgcattctc cgtactggtt tccgtctggg tgacaatgcc
     9901 gaaatgtgta tcatcgaact ggtggactac aacgaaaata tgttgaagga aaaaaccgct
     9961 aagaaatcga ctcgtacacg tcgctcttca aaaaaagctg atgtagcagt agctgctgaa
    10021 gataaagcaa aagctcctaa agctgctcct aaagttgaag aaaccaaggc agaagcaact
    10081 gttgaagaaa aaggcgaata agaacacttt ttcaactaaa agtaaaaaaa caaattctga
    10141 aaagggtttg tttttttgtt tttaaaatgt tctccacaga tgacaacatc tttagtatca
    10201 gttatcatca taattttacg gatatgtcac tatttttgct attatccgat attttgcaca
    10261 ttatcaggtt gatagagtaa taatataatc ggtcttacta aatgttgttt tacacttatt
    10321 attctcatca gtttcaatca actattcatg aacacatttg caaatcccaa ccaaatttca
    10381 tacttttgtt ttggaaaaga attaaaatgg ctatgattaa atatgaacgt atcctgctta
    10441 aactgagcgg tgaatcactg gcagctgatc aaaagcaggg catcgatatt gtccgtctcg
    10501 aacaatacgc ggcccagata aaggaaataa ccgaacttgg cgtacaggtt gggatagtaa
    10561 ttggcggagg aaatatattt cgcggattga gtggagcatc caaaggattc gaccgtgtac
    10621 agggtgatca gatgggtatg ctggccactg ttatcaacag cctcgcactc aactcggcac
    10681 tgcaggcggc aaaaacaaaa tcacgcgttt tgacctcggt ccgtatggaa cccatcggag
    10741 aattttacag taaacgctac gctgtggaat gtttggagag gggagaagtg gtaatcattg
    10801 caggcggaac gagcaatccg tatttcacca cagataccgc gtcctcgctt cgtgccatcg
    10861 aaattgaggc cgacatcatg ctcaaaggca cccgtgtgga tggagtttat accgctgatc
    10921 cggagaaaga taaattagcc gtgaaatttg aaaaaatttc ctatgacgaa atttacaata
    10981 gaaatctgaa agtaatggat ttaacggcta ctaccatgtg taaagaaaac aaaatgccga
    11041 tatatgtgtt tgatatggac acagttggca atcttaagaa agtgattttg ggtgagaata
    11101 tcggaacact ggtatattga tattaatata taacctctaa ataattaatt ttatggtaga
    11161 ttataaaaca catatcggcg atgcagagga actaatgcag gcatcggtag agtatctgga
    11221 tgaagcgctg gcgcatattc gagccggaaa agctaataca cgcatacttg atggagtgaa
    11281 ggttgagtat tacggatcat tggtgccgct ttcaaatgtt gctacggtaa caacacccga
    11341 tgccaaaacg atagccattc aaccttggga aaaatccctg atttctgtta ttgaaaaagc
    11401 aattttgaac tccagtgtcg gaattactcc tatgaacaat ggagaaacga tccgattggg
    11461 aattccgcct ctgaccgaag aacgaagaaa acagctggta aaacaatgcc gtcaggaagg
    11521 agaagaagct aagataagtg ttagaaatgc gcgtcgtgaa gcaattgaaa atgtaaagaa
    11581 actggtgaag gaaggtttgc cggaagatat ggaaaaagat gctgaagacg aagttcagaa
    11641 aattcacgat aaatatgtaa aaaaaataga tgaattgctg gcagcgaaag aaaaagaaat
    11701 aatgacagtg taatcggtca aattttacta aataatgtat tcgaatttac ggtcgtcccg
    11761 gtggagtggg atgaccgaaa ttttttatca gaatttttaa ataatgcagg gacttgtagt
    11821 aaaaaataca ggcagttggt acactgttcg aacagatgag ggtgaaatta ttgattgcaa
    11881 aatcaaggga aattttcgaa tcaaagatat tcgcagtacc aatccgattg ctgtcggcga
    11941 cagagtgaaa ctcactctca atactgatgg cactgcaatg attcacgaaa tctgcagccg
    12001 aaaaaactac attatccgtc gatcttccaa cctgtcgaaa tactcgcaaa tactggccgc
    12061 taatcttgac ttggtagcac tcgttattac tgtaaattat ccggaaacat ctaccgtttt
    12121 tattgaccgg tttttggcca ctgccgaagc atatcaggta ccagcttgtc tggtgattaa
    12181 taaaaccgac agatattctg aggatgaatc tgcatttgca gcggcattga aaaatcttta
    12241 tgagacaatt aattatccgg tgtttcttgt gtcagctatt acaggcgatt cggtgcctga
    12301 acttaaagaa tatttaagca ataaaacaac ccttctttcg ggcaattccg gtgtgggaaa
    12361 gtcaacgctt atcaatatga ttgcgccaaa ctctgatgta cgtacaaata aaatatcaga
    12421 gtatcataat aaaggtatgc acactacaac attttcggaa atgttcctac ttgacgatgg
    12481 gggatttatt attgatactc cgggtattaa agggttcgga acgattgata tgcaggagaa
    12541 tgaggtggga catttcttca gagaaatatt cagaatctca aaagaatgtc atttcaataa
    12601 ttgtacgcat acacacgaac cggggtgtgc tgtttttgat gccgttgaaa aacatctgat
    12661 aagccaatcg cgctatcaaa gttatttgag tattatggaa gactgtaaaa gcggaaaata
    12721 tcgataatgt atattaattg aaactaatgt aataatttga agtatttagt taggttgaat
    12781 caataaattt tgatacattt gtgtcggata gtataaagat ttaaaaataa aataagaaat
    12841 aacataaaaa acacaaagca atgggacatc atcaaattga caggctggat aaaaaaatcc
    12901 ttcaattaat atcaaaggat gccagaatac cttttttgga ggttgccagg gaatgtaatg
    12961 tttcgggagc agccattcat cagcgtatac aaaaattacg caaccttgga attataaagg
    13021 gatctgagtt tgttgtagat acgtacaaga ttggttatca gacctgtgcg tatatcggga
    13081 ttgtacttac ggacataaaa aagtttgact ctgtagtctc ggaacttaaa aaaatcccgg
    13141 aagtggttga atgtcattat gctaccggca aatactctat gtttataaaa atttatgcca
    13201 aagacaatcg ccatttgtta caaattatcc tggaacattt gaccttgatt gatggtgtag
    13261 gtcatactga aactttccag ttgtcactcg atgaaatttt taaacgtcag ctttctgttt
    13321 ttgaagttgg agaagatgat ctggatgata ttgaagatga gtaaaatctc taattaaata
    13381 gtcattaaaa aagccggatt tatttcggct ttttttgtct taaatacata gaatgttcat
    13441 cttcttatct gcgggagttt accgttttac ggttttttgg gagcgacagg cagataatca
    13501 gattgaggag tagttgactt aatgggccaa aatatagagt caggaaaagt cttgaacttt
    13561 attaatccca gactatccat attatagaat tcctcaatag tcttcaatgg atcaaatttt
    13621 gtcacttcta ctcttactgt tccatgaccg attaatccaa tctgtttggc agcagcatag
    13681 gataaatcaa cgattcgtcc ccttacaaat ggaccccggt cagtaacttt tacaatcact
    13741 tcttttccgt tgtttagatt ttggattttt aggagcgtac caaacggata gcttctgtgt
    13801 gcacaggtaa gactgtctcg atggtaaggt ttcccgttag cagttttccg gccgtgaaga
    13861 ttgttgccgt aataggatgc tttgccaatc tcctggctgt atgaacctat agctaatatg
    13921 aataaaatta atatgacagg aaatttatac cattccatta aaagtaaatt tataatgtga
    13981 cagaggttaa agtcaaaaca aagcaatttt tgtgccaatt tttgtgttta ttgctttttt
    14041 gtttatagtc aattaatttg ttcaccttac aatagtaacg tcaaaacagt taaattgttt
    14101 tgtgagttta atctgattgc ttatccccgg tattgtcgct ggttatataa ataatcatta
    14161 gagcattatt tttataattt aaaaaaatgt gttatatttg caccctaaaa caaaaaatgt
    14221 ttcattataa attttcagga ggaaacaagc aatagctaaa caattaataa tcaagccaag
    14281 gcaaggtcag aaagaaaaag atatgcctaa tcgcatcaat gagaaaatca aaggtgtaaa
    14341 ggaagtaaga cttgttggag acaatgtgga acaaggaatt gtttcgattg atgaagctct
    14401 ccgcatagcc gaagaactgg aattggattt agttgaaatc tcaccctcag cagtacctcc
    14461 ggtttgtaaa attgttgatt atcagaagtt tctttatcaa cagaagaaac gtgaaaagga
    14521 acaaaaggca aaagctgcaa aggttgtact gaaagaaata cgtttcgggc ctcagacaga
    14581 tgaacatgat tacaatttca aactgaaaca tgcgatcagt tttctgaagg aaggttgcaa
    14641 agtgaaggct tttgtgtttt ttaagggaag atcaattttg ttcaaagaac aaggcgaagt
    14701 gttactactc cggtttgcca atgatctgga agattatgca aaggttgatc agctccctca
    14761 gttggaagga aaaagaatga ttatattctt atctcccaaa aaaacaaaat gattgaattt
    14821 caataatata taaaaaagag aaacgatgcc taaaatgaaa actaactccg gtgccaaaaa
    14881 aagattcgct cttaccggaa caggaa
//
LOCUS       DAOPYB010000060        14868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4360_length_14868_cov_6.333221, whole genome shotgun sequence.
ACCESSION   DAOPYB010000060 DAOPYB010000000
VERSION     DAOPYB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14868
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4360_length_14868_cov_6.333221"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKH58_06400"
     CDS             complement(<1..275)
                     /locus_tag="PKH58_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88693.1"
                     /translation="MKKQFLFIPLFWILLISCNNKSELKDTNIIAKFVTDSLLNGYAI
                     DSLDIYDMDSSFLFASNHPSYIALYDELMVDIRELISKVDYYNFEKNV"
     gene            complement(1311..2045)
                     /locus_tag="PKH58_06405"
     CDS             complement(1311..2045)
                     /locus_tag="PKH58_06405"
                     /inference="COORDINATES: protein motif:HMM:NF040365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6261 family protein"
                     /protein_id="HNX88694.1"
                     /translation="MVKLAYKSKVHKLSNKSVGILVLRLFEASVAANVALVINSKSYT
                     ALTEVNNRYQASVDPTKYKDQTEAIDAKANERRAYFLLNYGYLKGLLNAPDAEQRAAA
                     VRIFAVMNMYGRNFNNLKIAEQSFLFNRILEGLQKPELAADLETTRLTASVAELDRLH
                     KEYETLFALRGNEVIGVVAPSTLRPQLNVAVNNFVEELQWMAQINDTEELKSLRDIVL
                     MRVSEVNLSQTQAKEPITEGNGTTVA"
     gene            complement(2332..2742)
                     /locus_tag="PKH58_06410"
     CDS             complement(2332..2742)
                     /locus_tag="PKH58_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88695.1"
                     /translation="MKQDTLNNKSNNMTENRTFSDIRDLFSFEAKDFNWDDVQVFESW
                     NDILSFKDFLPDRVKFDASSLFNLLISYTLQGKYHARLCAMARLLATPVISIKTLKQS
                     LRASISLLFGMCSSYVSYIYSAIFGNSSTRILSI"
     gene            3566..4075
                     /locus_tag="PKH58_06415"
     CDS             3566..4075
                     /locus_tag="PKH58_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88696.1"
                     /translation="MKKVFLVVVVSICMGILTGCSGKTEKSGKSSVAENNSAISLNEL
                     PGAVNDFIALNYPSYKIKNAGHDPLCSGEDAMDVAIGKAGSPNFSLIFLPDGKFVQQE
                     EDVKLAATPAKILNTVKTKYPGFTAENGAEKLTLADKKTVHYQLDLIKEGTSKEVIFD
                     MEGNVVCEN"
     gene            4230..5492
                     /locus_tag="PKH58_06420"
     CDS             4230..5492
                     /locus_tag="PKH58_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX88697.1"
                     /translation="MKRFIFIALILTCLSGNQHLTAQNAKSGEDYIKAALLNSPLLYD
                     YENRRLSLKFDSALLMADYGLKVDAFADLMAAPVIHGWGYDEALSNGQFATTGLRVSR
                     DIIGRNNLKSRLDSYKLSENQLNSQKQLSEQMMRLTVTDQYINTYISQEQLSIYQEII
                     NVLKKEEKILYKLTQSAVFSQTEYLTFLVSLQQNELSLQQAKADFQNNMSVLNVISGL
                     MDTTTYSLSPPLMDEKMAVPFNESIYAQLNQSDSLKLENEAIIIKNEYKPKLSVYADA
                     GYSSSFIKSPYKNFGTSVGLNLSIPLYDGNKQKMLLQQNSITQDTRSRYYAFNQQQYA
                     QKIYQLNQQIQQTRQMLNTCDEQLKYSDTLIKANLKQLPTGDIRVTDFIVSENNFLNL
                     KLTRIQYEGKLYSYFNQLKYMSISTPAP"
     gene            5553..6530
                     /locus_tag="PKH58_06425"
     CDS             5553..6530
                     /locus_tag="PKH58_06425"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX88698.1"
                     /translation="MKYIRFSNQYNSPFKGEGGKYIFLPLFLIPVMLVGCKANNANEE
                     NAPVQAKTSVSVTYPTDTVMLIDQIELNATATYLLKTDIKANTTGYITDVKIKPADWV
                     KSGQNLFIIQTKEAKAIGNTINELDPSMNFSGHTSVASPTTGYVQMLNHQIGNFVQEG
                     ETLATIADGSSFGFLMIVPYEYNQLVNSNKNLTIELPDGRKLPGYVDKIMPTVDPVSQ
                     TEQVLVKLKSKVSIPENLIVSIKLIREQDQELSVPVEALLTDETQSIYWVMKLINDTT
                     AVKTIVQKGIETDKWIQIEAGDLTKNDRIIVSGNYGLNDTALVKITKTR"
     gene            6676..9711
                     /locus_tag="PKH58_06430"
     CDS             6676..9711
                     /locus_tag="PKH58_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX88699.1"
                     /translation="MKKRHSFYVRYKTPILMLALIIVVTGVFSYLNMKSSLFPQTTFP
                     KIKIIADAGQQPVDQMTATVTKPLEEAVKQIPNLLVVRSTTSRGSCEISAFIDWKADV
                     DLSVQQIESRISQVRNQLPSDVNITVEKMNPSILPVMGYSLTSQKTAPFELKKLALYT
                     IKPFLSQVPGVSEVRVIGGQEKEYRVVLDQMKMSKLGITPSDIADIMSKTNFIQSNGY
                     FTDYKYLYLTLTDTQLKNLDQLRNIIISNDGSRIVYFSDIADIELHQAKQYIKVNANG
                     EESILIAVVQQPNANVVEVSRAMNIKIKELKKSLPNDIELKSYYDQATFVNDAIRSVS
                     DALWIGLFLAIVVAILFLRSWKSSLTILITIPVTLLLTILILYITGQTFNIMTLGAIA
                     ASIGLIIDDAIVVVEQIHRTHEENPEESSEDLIHVAISYLFKAMVGSSLSTIVIFIPF
                     VLMSGVAGAYFEVMTNTMLITLVCSFFTTWVLLPVIYLFLSKFFKEKKLRSHDVKERK
                     WVGYFIDHPVYSYVFMALVVLSAIVILPRLSSGFLPEMDEGSIVLDYKSVPGTSLEET
                     DRELREVEKILAEIPEVEAYSRRTGTQMGFFITEPNDGDYLIQLKKKRNKTTVEVIDE
                     IRSRIEAHTLPLTVDFGQVIGDNLGDLMSSVQPIEIKIFGKDNDIIQDYSRQVAAIVS
                     RVKGTADVFDGILIAGPSAQITPDLTALGQYGISPQDFQYQVQTQIEGNEVGKIFEKQ
                     QYTPIRIMYSHNSNSSFTQIQNSLISLPTGQLKPLKEFADVKIIPGVAEINREDLQNI
                     GIVTARLDNANIGGTVKEIQKEINKQIKLPTGYSIVYGGAYAEQQQSFSELLMILILS
                     SLLVFTVILFLYQNVVVAGLILLISIMGIAGSFIFLFITNIPLNVGSYIGLIMMVGII
                     GENAIFTYLQFQSNLQQSSKKEALIYAISTRLRPKLMTAIGAIIALMPLALGIGTGAQ
                     MHQPLAIAVIGGFILALPLLLIVLPTMLNKIKFKEEIKV"
     gene            complement(9914..14422)
                     /locus_tag="PKH58_06435"
     CDS             complement(9914..14422)
                     /locus_tag="PKH58_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX88700.1"
                     /translation="MKNPGYYVLIVSLLFLSIFTLSAQNEWDDVKITQVNRENACTVG
                     IPFETENSLLTNSTEQSSYYLSLNGVWKFKWVSDPSKKPADFQNPTYNVSNWDNIDVP
                     LNWQIYGVRNGKSWDKPLYVNTRYPFTYDSNYSVMANRPTDYTYNNNMKNPVGSYRRE
                     FTLPANWDGRDIFVRFNGAGPGYYLWVNGQQVGYSEDSFLPSEFKITDYVTSGTNVIA
                     VQVYRFTSGSFLECQDFWRFSGIHRDVFLWSAPKTQIRDYFFTSNLDNSYTNAAVSID
                     VELSGTPLTNSKITAKLLDNGSVIATKELTSPVIGKNTIAFPVYSPKKWSAEIPNLYD
                     LVLSLEDNTGKVLDIRGGKAGFKKVEVGSKGELLINGKRMVFHGVNRHDHSEINGRTV
                     SKEEMERDVKTMKQLNINAVRTSHYPVNPYFYELCDKYGLYVLSEANVECHGNTGLSG
                     VEVFRKPMVERNQNMVKRFRNHVSIFMWSYGNESGGGNNFYYVENAIKALDKTRLTHY
                     EGNSQWSDVSSTMYADINRIKTIGEQRLTEAKPRPHIQCENSHAMGNAMGNVREFFDL
                     YEKYPALTGEFIWDWKDQGIKMPVPNKPNEYYWAYGGDFGDSPNDGSFCTNGVVFADN
                     SFSAKSYSTKKVYQPIDFFMKDDQKTFILKSKRAFKPTDDLDIYYSVFEDGKEIKRNK
                     LDVTLEGGATTEIILNVLPETPKPEAEYFIRFNAYQKEATWWAPQGYEVAGEQIKLKE
                     AIKPIASIPATGTLTVTSTSVDITVTGSNFSAVFSKSAGTLIKYTYNGKLLINQPLSL
                     NVFRLPTENDKPQTKGWDDMGIRLLTVKAGVWDIQENTNTVDLSILNTYTAIAPTVFT
                     TRMLFKIATDGNIYVSSIIDPLKKNVILPKIGFRLEMPKDFEKLTWFGRGPWESYVDR
                     REACFEGVYNSTATDQWEKYVLPQETGNKEEVRWMSLTDNAGDGLLYVAPQQMSATAT
                     HFRPEDIYRDRYNREKHPYQAKLKFCENTVVSLNATMRGLGNATCGPDVLEKYELKAD
                     NTIFNFMIMPVAGNLNNDQLSERARVQFPICNAVDIVRNNQGKIVLTTTTPNARIYYS
                     IDNGAFQLYQGSFEFLSAGNIVSYCESDGFLSGLKTSVDFGLFIDKSAWKVLSYSSQA
                     SGEEAYKSIDGNESTIWHTSWGTYEPVHPHEIVVDMQKTYRVEAFNYTGRQDGENGRI
                     KEYEIYFSNDPNVWGSPAAKGEFTSTTAQQSVIISSKPVARYFRLIAKSEVNGKAWTS
                     ASELGIEASAIVNPESVSCTTPDKTKKYYLKHLQSGLYLQLLPDVSTNYEGDFCLSAL
                     DKSNNTFEFDFTPVAGFTSFYNVRVTDTYINQKDSWRCVSGIKKDNNGMIQIETLADC
                     TVKLRGVWQKWDYINLDNTVAGSYIYGNKPAGAIWQLEEVISGTGVSTVNNSAGVSIY
                     PTLTTGKVRILTHDKSTIKILDFSGRTKDTYSSAGDLTVNMNYPNGIYLFQINSGSTS
                     THKVMLSK"
BASE COUNT         4499 a         2956 c         2950 g         4463 t
ORIGIN      
        1 acattttttt caaaattata gtaatcaact ttacttataa gttcgcgtat atcaaccatc
       61 aattcatcat ataacgctat atatgatggg tgattagaag caaacaaaaa agagctatcc
      121 atatcatata tatctaaact atctattgca tatccattca acaagctatc tgtaacaaat
      181 tttgcaataa tatttgtatc ttttaattca cttttattat tgcacgaaat tagaagtatc
      241 caaaataatg ggataaacaa gaactgtttt ttcatggtta atttgtttta gtattaattc
      301 ttacttaaat aattatattg ttttatgtgt gagttattat tactttttcc cagaagttga
      361 agatatttat ctggaccttg cttaaaagca aatattcttt attcttaaag ctattttcag
      421 tacaagcaat gccttaacaa aaataatact aaaacatttt cagctaaaac actacaaata
      481 tagtgaaata ttataaatac aaataatatt taatatatgt aataaactaa atcaaaacct
      541 tttatttatt taactaagat gttgtgtgag ttgttagcgt caagcatgcc aataacgata
      601 aggattatcg ctttagatat tttttcttaa gcggttagga aaccgcttgt ccactgttag
      661 ttttatacaa tttctcttcc ataataattt tgaacaagaa ttgtttcatg tgggtgtcgt
      721 tctttgcaac gtcccctcca taaccaaaaa tcatcccgtt atttttatca acctgagcac
      781 cggggctagc aaaaaaaaac tttttattgg catttttcac tcaatgtctg agaataaaaa
      841 cattttattt aataaattcc actcaatatc cgggcaaaaa aaacattttg tgagcatttt
      901 caaaccaata tctcgtcaca aacagttgtt ttttctcatt tttcattcaa tatctatgaa
      961 aaaacggttg ttatttttac atcctttcac aatggaaagg aacaaacatg tttgttttta
     1021 aagatattgt taagggaatt taaaaagaag ggttatattt gttagacatt ggatgtggga
     1081 aatgaaaaaa atacaatttg taaccaaaca ttgagtgtag gaaaggaaaa aaaatcattt
     1141 tgcaacaaga aattgagtgt aggaaaagaa aaaaatgttt ttgacaacaa gaaattgtac
     1201 ggtgatatgt cgtagtaata tgcagataat caggcgacag attaaaaata agaaagcctg
     1261 taccaaaggg cacaggcttt ctacatagtt aagagacctt aaatttaact ttatgcaaca
     1321 gttgtgccgt tgccttcggt aattggttct ttcgcttgcg tctgacttag attgacttca
     1381 gaaacacgca tcaggacgat gtcgcgcagc gacttcagct cctccgtatc gtttatttga
     1441 gccatccatt gcaattcttc cacaaaattg tttactgcaa cattcagttg ggggcgtagg
     1501 gtagagggag caactactcc gataacctca ttgccacgca aggcaaacaa cgtttcgtat
     1561 tccttgtgaa gtctgtccag ttcagccacg ctggcagtca gcctggtggt ttcgaggtcg
     1621 gcagccagtt cgggtttttg cagcccttcg aggatacggt taaacagaaa cgactgttca
     1681 gcaattttca ggttgttgaa gttccgtccg tacatattca tcactgcgaa aatccttacg
     1741 gcggcagctc tctgttctgc atcgggtgca ttgagcaacc ctttcaaata cccatagttt
     1801 aacagaaaat aggcacggcg ctcattggct ttagcatcta ttgcttcggt ctggtccttg
     1861 tactttgtcg gatcaacgct tgcctgatac ctgttattca cttcagtaag tgctgtatag
     1921 ctcttactat tgattactaa tgcgacattg gctgctacgg atgcttcgaa caaacgtaat
     1981 accagaatgc ctactgattt gtttgaaagt ttatgaactt tcgatttata tgcgagtttt
     2041 accattatca aaaattttaa tattgttatc agagatgaac cgaattagtc tgctgtttca
     2101 ttgattagct attgtcggac ctgttccatc ctgctcccaa acttagttgg ggttttcagg
     2161 ggtaaaagtc tcacaatcaa ttgacaggaa gacgacagaa aaatttacta taactatctg
     2221 ctccggataa tctcaattga atttatttaa agaaaacgtg attcagtttc tccggaaacg
     2281 gggaaacagg ggtacacacg gagtcataaa cccggatgta tcggtatgga tttaaatgga
     2341 taaaattcgg gtggaggaat tgccaaaaat ggcggaataa atataggata catagctgct
     2401 gcacattcca aaaagcagtg aaatggaggc tctcagcgat tgctttaatg ttttgatgga
     2461 aataacggga gtagcgagca ggcgtgccat agcacataat cttgcatgat attttccctg
     2521 gagtgtgtaa ctaattaata agttgaataa ggatgacgca tcaaatttta ctctgtcggg
     2581 caaaaaatcc ttaaaactga gaatatcatt ccaactctca aagacttgta catcatccca
     2641 attaaaatct ttggcctcaa acgaaaataa atccctaatg tccgaaaatg tcctgttttc
     2701 agtcatatta ttggatttat tattgagtgt gtcctgcttc attttttatg tgattaatgt
     2761 agaatatgta taaaataata ctaacaaata ctgtgccaat gttaaaaatg cgccaatatg
     2821 gcaggttttt acctggctgt cagaaaaaat gctaataaaa agaaaaaact gacaaaattg
     2881 tcacccggaa acagacaaaa taatgagccc gccatatttc ctgtcagaaa aacagctgat
     2941 aaaaagcaaa aactaacaaa attgtatctg attagttaga gtgagctcaa tatgaacaaa
     3001 cctttgaatt ttaagtcaga aaatacaata gtctgtattc ccgtagggaa ttaatatcaa
     3061 tagaaccagg ttcttaaata aattaaattc cccgttaagg gataaatata tccattccct
     3121 acgggaaatc aatcaaaaca aaaacattct ttctattgat atttaagtcc tacggactat
     3181 tgcttatgtc gaacttaggt taattataaa atatagacga cagagctacg ctcctttgat
     3241 tattcggtga ataatattct gcaaagctgt ttgcagcacg cagggacaac agaactaacg
     3301 cccctgaaac agatatatgt ggaacggcat agcttaacat aaaataatgc tggcgggttg
     3361 agacatttaa gaaaaccgaa taaatttcgc ccggtaggtt aaataatcag aaaaataacc
     3421 aaatatttaa ctaatagaga gtaccattat tatatatttc actacttttg atattgggtt
     3481 caggattaat tcctgtttgc cttccggaat tgcaaaagat atattatcct ggcatattaa
     3541 acaatttctt aaataaaaaa atgaaatgaa aaaggtattt ttagtagtcg tagtttcaat
     3601 atgtatgggg atactgaccg gttgttccgg aaaaacagaa aaatccggaa agtcatccgt
     3661 agccgaaaac aattcagcta tttcgttgaa tgaacttccg ggtgctgtga atgattttat
     3721 tgccttgaat tatccgagtt ataagataaa gaatgccggt cacgatccgc tttgcagtgg
     3781 cgaagatgcc atggatgttg ccataggtaa agccggaagt ccgaactttt cgctgatatt
     3841 tttaccggac ggtaaatttg ttcagcagga agaagacgtg aagctggcgg caacacccgc
     3901 caaaattctg aatacagtaa aaacaaaata cccgggtttt acggctgaaa atggtgccga
     3961 aaaactaacg ctggcggata aaaaaaccgt tcattatcaa ctggacctca taaaagaagg
     4021 aacttcaaaa gaggtgattt ttgacatgga aggcaatgtg gtctgcgaaa attaatatgg
     4081 ctacaaatcc gccatcttac ggaggagtaa gttggctaaa tgaacaaaca tctatataag
     4141 aactatttaa ctataatacg ttgaaattct aactacttta atatacgctt tcaactcccc
     4201 ctttaggggg gtggggggct cttaccaaaa tgaaacgttt catctttatc gctttaattc
     4261 tgacctgtct gtcgggtaat caacacctta cggcacaaaa tgccaaaagc ggtgaggatt
     4321 atattaaagc ggcgctcttg aacagtccgc tgctgtacga ttatgaaaac cggaggttgt
     4381 cactgaaatt cgacagtgcc ctgcttatgg ctgattacgg tttgaaagtg gatgcatttg
     4441 ccgacctgat ggctgcgccc gtaatacacg gatggggata cgacgaggcg ctctccaacg
     4501 gacaattcgc taccacagga ctgcgtgtaa gccgggatat tattggaaga aataatctga
     4561 agagccgtct ggatagttat aaattatcgg aaaatcagct gaatagtcaa aagcaactca
     4621 gtgagcagat gatgcggttg accgttaccg atcagtacat caatacctat atatcacagg
     4681 aacaactgtc aatctatcag gaaattataa atgtactgaa aaaggaagaa aagatattgt
     4741 acaagctgac ccagtcggca gttttttcgc aaaccgaata cctgacattt ttagtctccc
     4801 tgcagcaaaa cgaactttcg ttgcagcagg cgaaagccga ttttcaaaac aatatgtctg
     4861 tactcaatgt catttcggga ctaatggata caaccaccta cagcctgtca cccccattga
     4921 tggatgaaaa aatggcagtt ccattcaacg aaagcattta tgctcaactc aatcaaagcg
     4981 acagcctgaa gcttgaaaat gaagccatta ttattaagaa tgagtacaaa cccaagttat
     5041 cggtttatgc cgatgcgggg tattcatcga gctttataaa atcaccctat aagaattttg
     5101 gcacaagtgt gggtttgaat ttatccatac ccttatacga tggaaataaa cagaaaatgc
     5161 tccttcaaca aaattcaatc actcaggata cccgttcgag atactacgct ttcaaccaac
     5221 aacaatacgc acagaagata tatcaactca accaacaaat ccagcaaaca cggcaaatgc
     5281 taaatacatg cgatgagcag ttgaaatatt ccgataccct tataaaagcc aatctgaagc
     5341 aacttcccac cggagatatc cgcgtgacag attttattgt ttcggaaaat aattttttga
     5401 atttgaaatt gaccagaata caatatgaag gaaagttgta cagctatttc aatcagttga
     5461 aatacatgag tatcagcact ccggctccct aaaggaggag tatggagtat gaattgaagt
     5521 ttatagataa tagaattata ttttattcaa ttatgaaata catcaggttt tccaaccaat
     5581 ataattcccc ctttaaaggc gaagggggca aatatatttt cttaccactg tttctgatac
     5641 ctgttatgct cgttggctgc aaggcgaata atgccaatga agaaaatgct cccgtgcagg
     5701 ccaaaacgtc ggtttcggta acttatccca ccgatacggt gatgttgatc gaccaaattg
     5761 agctgaacgc tacagccaca tatctgctca aaaccgatat aaaagccaac acaaccggtt
     5821 acattaccga tgtgaaaata aaaccggctg attgggtgaa aagtggtcaa aacctgttta
     5881 ttatccaaac caaagaagcg aaagccatcg gaaataccat taatgagttg gacccgtcga
     5941 tgaatttttc gggacacacc tcggtggcaa gtcctacaac gggctacgtg caaatgctga
     6001 accatcagat cggtaacttt gtgcaggaag gcgaaacact tgccaccatt gccgatggaa
     6061 gcagctttgg atttctgatg attgtgccct atgaatacaa tcaactggtg aacagcaata
     6121 aaaacctgac catagaactc cctgacggac ggaaacttcc cggatatgtg gacaaaatta
     6181 tgccaacggt agatcccgtt tctcaaaccg aacaagtatt ggtgaaactt aaaagtaaag
     6241 tttccattcc tgaaaatctg attgtatcca tcaaacttat ccgagagcaa gatcaggaac
     6301 tttcggtgcc ggttgaagca ctgttgaccg atgaaacaca gtccatttac tgggtaatga
     6361 aactgataaa cgacacaaca gcagttaaaa ccattgtgca gaaagggatt gaaactgaca
     6421 aatggataca gattgaagcc ggagatctga ctaaaaatga ccggattatt gtttcgggca
     6481 attacggatt gaatgataca gctttggtca aaatcactaa aacgcgctaa tcagccgggg
     6541 gaaagggagc attatccatg ctggctggag gtacgatttt tttattccat gtttttattt
     6601 ctgatgcatt tttagaaccc ttccctaatc cccttttaag ttaagatggc taatatctga
     6661 agacttgtat aaattatgaa aaagcgacat tctttctatg taagatataa aacgccaata
     6721 ctgatgttgg cgctaataat tgtagttaca ggcgtttttt catacctcaa tatgaaatct
     6781 tccctgtttc cacaaaccac tttccctaaa atcaaaatca tagcagatgc cggtcagcaa
     6841 cctgtggatc aaatgacagc cacagttaca aagccactcg aggaagctgt gaaacaaata
     6901 cctaacctgt tagtggttag aagtaccacg agccgtggca gctgcgaaat ttctgccttt
     6961 atcgactgga aagccgatgt ggatttaagc gttcagcaga tagaaagccg catcagtcag
     7021 gtcagaaacc aacttccttc cgatgtgaat atcacggtgg agaaaatgaa cccgtccata
     7081 cttcccgtga tgggttattc gctgacttcc caaaagacgg cgccgtttga gttgaaaaaa
     7141 ctggccttgt acaccatcaa accatttctc tcgcaagtgc ccggtgtgag tgaagtgcgg
     7201 gtgattggcg gacaggaaaa agaatatcgg gttgtattgg atcagatgaa aatgagcaag
     7261 ttgggtatca caccatccga cattgccgac atcatgagca aaacgaattt tattcagtcc
     7321 aatggttatt tcaccgatta caaatacctc tacctgacac ttaccgatac tcaactaaaa
     7381 aatttagacc aactgagaaa tatcatcatt agtaatgatg gcagccggat tgtctatttc
     7441 agcgatattg ccgatataga actgcatcag gcaaagcaat acataaaagt caatgccaat
     7501 ggcgaagaaa gtatactcat tgctgtggtg caacaaccca atgccaatgt ggtggaagtt
     7561 tcccgggcga tgaatatcaa aattaaagag ttgaaaaaat cgcttcccaa tgatattgaa
     7621 ctgaaatctt actacgatca ggctactttc gttaatgatg ccattcggag tgtttctgat
     7681 gctctctgga taggattatt tttggccata gttgttgcta tactattttt aagatcgtgg
     7741 aaatcaagtt taaccattct tataaccatt ccggttacgc tgttgcttac tattcttata
     7801 ttatatatta ccggtcagac tttcaatatt atgaccctgg gagccattgc cgcatctatc
     7861 gggctaatca ttgatgatgc tattgtagtt gtggagcaaa tccaccgtac ccacgaagaa
     7921 aatcccgaag aaagcagtga agacctgata cacgttgcga tcagttatct tttcaaagcg
     7981 atggttggtt catcgttgag cacaattgta atattcattc cttttgtact gatgagcggt
     8041 gttgcgggtg cttattttga ggtaatgaca aatacaatgc ttattacact cgtttgttca
     8101 ttttttacga cgtgggtttt gctgccagtc atttatttgt tcttatctaa gtttttcaaa
     8161 gaaaaaaaac tccgctctca cgatgttaag gagagaaaat gggtgggata ttttatcgat
     8221 catccagtgt attcgtatgt gtttatggct ctcgttgtac tttcagcaat tgttatttta
     8281 ccccgtttga gttctggctt tttgcctgaa atggatgaag gaagcattgt gctggattat
     8341 aagtctgttc cgggaacttc gctggaagaa accgaccgcg aactacgtga ggtggagaaa
     8401 atactggccg aaatacccga agtggaagcc tacagccgac gtacaggaac gcagatggga
     8461 ttttttatta ccgaacccaa cgacggcgac tatttaattc agctgaaaaa gaaacgaaat
     8521 aaaaccactg ttgaggtaat tgatgaaatc agaagccgga ttgaagcgca tacattgccg
     8581 ctgactgtag atttcggtca ggtgataggc gataatctgg gagacctgat gagcagtgtt
     8641 caaccaatcg aaataaagat attcgggaaa gacaacgata ttattcagga ttattcacgc
     8701 caggtggctg ctattgtgag ccgtgtgaaa ggtactgccg atgtatttga tggaattctg
     8761 atagcaggcc cttcggctca gattaccccc gatctgacgg cattgggaca atatggtatt
     8821 tcccctcagg attttcagta tcaggtgcag acacagattg aaggaaatga agtgggtaaa
     8881 attttcgaaa aacaacaata tacgccaatc cggattatgt actcccacaa cagtaattcc
     8941 tcctttactc aaattcagaa tagtttgatt tcgctgccaa caggacaatt aaagccgctg
     9001 aaagaatttg ccgatgtgaa aattattccg ggagtggctg agattaaccg ggaagattta
     9061 cagaatatag ggattgtaac agcccggctt gacaatgcca atattggcgg caccgtgaaa
     9121 gaaatacaaa aagaaattaa caaacaaatc aaacttccca ccggttacag catcgtttat
     9181 ggcggagctt atgctgagca gcagcaatcg ttcagcgaat tgttgatgat tttgatactc
     9241 tcaagtttgc tggtttttac ggttatactt tttctgtacc aaaacgtagt ggtggcggga
     9301 cttattcttc ttatttccat aatgggtatt gcaggaagtt ttatatttct ttttattacc
     9361 aacatccctc tcaatgtagg cagttacatc ggactgataa tgatggttgg gattatcgga
     9421 gaaaatgcca tcttcaccta cctgcaattt cagtccaact tacagcaaag cagtaaaaaa
     9481 gaagctttaa tctatgccat cagcacccgt ctgcgtccga agttgatgac agccatcggt
     9541 gccatcattg cactgatgcc gctggcatta ggcatcggca ccggagcaca aatgcaccaa
     9601 ccgctggcta ttgccgtaat cggcggattt attttagcgt tgcccttatt actaattgtg
     9661 ctgccgacca tgttgaataa gataaaattc aaagaggaaa taaaggtgta gtatgaattt
     9721 acaccgatat tacccttagc aggtacacta tcctgtccct gctactgcaa cttagggtaa
     9781 gtgttgaatt cacaaactaa aaatatggag gatgtaaatt tcagaagtat ctgttagatt
     9841 ttcttttcgg gcttaatgtc aggagcgagg acgtttgata cgctggttgt ggggagaaat
     9901 gaagattgat ttactatttg cttagcataa ctttatgggt ggatgtggaa ccggaattta
     9961 tctgaaaaag ataaattcca ttgggataat tcatatttac agtcaggtca ccggctgagc
    10021 tataggtatc ctttgttctg cctgaaaaat ccaggatttt gattgttgac ttatcatggg
    10081 taagaatacg tacttttccg gttgtaaggg tgggatatat agaaactccg gctgaattat
    10141 taaccgttga gacacctgtg ccactgatta cttcctccag ctgccaaatg gctcctgctg
    10201 gtttattgcc atagatgtaa gaaccggcaa cggtattatc gaggttaatg taatcccatt
    10261 tctgccatac accccgcaat ttaacggtac aatctgccag cgtttcgatt tgtatcattc
    10321 cattattatc ctttttaatg ccagagacac acctccaact gtccttctga ttaatatacg
    10381 tgtcagtcac gcgtacatta taaaacgaag tgaaaccggc aacgggagta aaatcaaatt
    10441 caaaagtgtt gttgctttta tccagggcac tgagacaaaa atcgccctcg taatttgttg
    10501 atacatccgg aagtaattgc agatataagc ctgattgcag atgttttaaa taatattttt
    10561 tggttttgtc cggtgtggta cagctaacac tctccggatt gacaatggcg gaagcttcga
    10621 tgcccagttc tgaagcagat gtccaggctt taccgttaac ctccgacttc gcaatcaacc
    10681 tgaaatacct tgctacgggt tttgaactga tgatcaccga ttgttgtgct gttgtgcttg
    10741 taaattctcc ctttgcagca ggacttcccc atacgttggg atcattgctg aagtatattt
    10801 catattcttt aattctgcca ttctctccat cctgtcttcc ggtataattg aaagcttcga
    10861 cccgatacgt tttttgcatg tcaaccacta tttcgtgggg atgaaccggt tcgtaagttc
    10921 cccaactggt atgccagatg gtgctttcgt ttccatcgat ggatttataa gcttcctcac
    10981 cgcttgcctg gctcgaatag cttagcactt tccatgccga cttatcaatg aacaggccga
    11041 aatcaacgct tgtctttaag ccgctgagaa atccatcgga ttcgcaatac gatacgatat
    11101 tccccgctga caaaaactca aaagatccct gatagagctg aaaggcacca ttatcaatac
    11161 tataatatat tcgtgcattg ggtgtggttg tggtcagtac tattttaccc tgattatttc
    11221 ttacaatgtc taccgcatta cagattggaa attgaactct tgcccgttcg gataactgat
    11281 cgttgttcag attacctgct acaggcataa tcatgaaatt aaaaatggta ttgtcagctt
    11341 tcaactcata tttttccagt acatcgggac cacaggtcgc attacccaat ccacgcatag
    11401 tggcgttgag acttaccacg gtattctcgc aaaacttcag tttggcctga taagggtgtt
    11461 tctcacgatt atatctgtcc cgataaatat cttcgggtct gaaatgagtt gccgtagcag
    11521 acatttgctg aggtgccaca tacaaaagtc catccccggc attatcggtc aggctcatcc
    11581 atcgcacttc ttccttattg ccggtttctt gcggaagtac atacttttcc cactgatctg
    11641 ttgctgtaga gttataaact ccttcgaaac aagcttctct ccggtctaca tacgactccc
    11701 acggaccacg tccaaaccag gtcagtttct caaagtcttt aggcatttcc aaccgaaaac
    11761 caatcttagg taatatcaca ttctttttga gtggatcaat tattgaactt acgtagatat
    11821 ttccgtcggt ggcaatttta aaaagcatcc gggtggtaaa tactgttgga gcaatagcgg
    11881 tataggtgtt cagaatgctt aaatcaaccg tgtttgtgtt ttcctgaata tcccagacac
    11941 ctgctttaac ggttagaagt cttataccca tatcatccca gcctttggtt tgtggtttgt
    12001 cattctctgt tggcaatctg aaaacattaa gcgacaaagg ctgattgatt aatagttttc
    12061 cattgtaggt gtacttaatt aatgttcccg ctgattttga gaacacagct gaaaaattac
    12121 tgcctgttac tgtaatatcc acactggtgc ttgtaaccgt aagagttcct gttgccggaa
    12181 ttgaggcaat tggtttaatg gcttctttca gttttatttg ttcacccgcc acttcgtatc
    12241 cctgtggggc ccaccaggtt gcttcctttt gataagcatt gaagcggata aagtattcgg
    12301 cttcgggttt gggagtttcg ggcaggacat tcaggatgat ttcggttgta gctccacctt
    12361 caagtgtcac atccagtttg tttcttttaa tctcttttcc atcctcaaaa actgaatagt
    12421 aaatatccag atcatccgtc ggtttgaatg cccgtttgct tttaagtatg aaagtctttt
    12481 ggtcgtcttt catgaaaaag tcaatgggtt gatatacttt tttggtgctg tatgattttg
    12541 ctgaaaatga attgtcggca aataccactc cattggtaca aaagcttccg tcattgggcg
    12601 aatcgccgaa gtcacctcca taagcccagt aatactcatt cggtttattg ggaacgggca
    12661 tttttattcc ctgatctttc cagtcccaga taaactctcc ggttaatgcg gggtatttct
    12721 catataaatc gaaaaactcc cgtacatttc ccatggcatt tcccatggcg tgactattct
    12781 cacactgtat atgaggacgc ggttttgctt cggtcagtcg ttgctccccg attgttttta
    12841 tccgattgat atctgcatac atggtgctgc tcacatcgct ccactgactg tttccttcgt
    12901 aatgtgtcaa acgtgttttg tccagtgcct tgatagcgtt ctctacgtaa taaaaattat
    12961 tgccacctcc cgattcattt ccatacgacc acataaaaat ggatacgtga tttcggaatc
    13021 gttttaccat gttctgattt cgctcaacca tgggttttct gaaaacttcc acacctgaca
    13081 atccggtatt cccgtgacat tccacattgg cttcggataa tacatacaag ccgtacttat
    13141 cgcacagttc gtaaaagtat ggattgaccg gataatgcga tgttctcacc gcgttgatgt
    13201 ttagctgttt catggtcttt acatcccgtt ccatttcttc tttcgataca gttcgaccat
    13261 tgatctcgct gtgatcgtgg cggtttacac cgtgaaaaac cattcgcttc ccgtttatca
    13321 acaattctcc cttactccct acttctactt tcttaaatcc tgcctttcca ccgcgtatat
    13381 ccagtacttt gccggtatta tcttcgaggc ttagcaccaa atcatagagg tttggaattt
    13441 cggcactcca ttttttcgga ctgtataccg gaaaggcaat tgtgttttta cctataaccg
    13501 gtgatgtaag ttcttttgtt gctataactg aaccgttatc cagtaatttt gcagtaatct
    13561 tactattggt cagtggtgta ccgcttaatt caacgtctat ggataccgct gcatttgtat
    13621 agctgttatc gagattagag gtgaaaaagt aatcccggat ttgagttttg ggagctgacc
    13681 agagaaatac atctctgtga atgccactaa agcgccagaa gtcctgacat tccagaaagg
    13741 agccacttgt aaaccggtat acctgtaccg ctattacatt ggttccactg gttacgtaat
    13801 cggttatctt aaattcggaa gggaggaaag agtcttcgga gtaaccaacc tgctgaccgt
    13861 ttacccaaag gtaataaccc ggtccggctc cattgaaacg tacaaaaata tcgcgaccat
    13921 cccaattagc aggaagtgtg aattcacgcc gataactgcc caccggattt ttcatgttgt
    13981 tattgtaagt gtaatcggta ggtcgattgg ccatgacact gtaattgctg tcgtaggtga
    14041 atggatatct ggtgtttaca tacaggggtt tatcccagct tttcccgtta cgaacgccgt
    14101 agatttgcca gttgagcggc acgtcaatat tatcccagtt gctgacatta taggtaggat
    14161 tctgaaaatc cgccggtttt ttggatggat ctgacaccca tttaaacttc caaactccat
    14221 ttagtgataa atagtaagaa gattgttcag tactgttggt taacaacgaa ttttcggttt
    14281 caaaaggaat gcctactgta caggcgtttt ctctgtttac ctgagttatt ttcacatcat
    14341 cccattcatt ttgagcagaa agagtaaaaa tagataaaaa taataacgac acaatcaata
    14401 cgtaataacc gggatttttc atgttttgtg attttttcat ttagtagtat agactaagtt
    14461 agtaaagttt actttgagga aagtagagtt tgtttctcag agcaggtgag gacggttttt
    14521 ccctatgcta cacctatgaa caagactaat attgtattcc gatcaaagtt aacgtgtgct
    14581 ttttaagcag ggaacaaaga tatgcaattt gcttaatgaa tcaaaatagt taatcaccaa
    14641 acaaaagacc ttgcaattaa ttaaggtaaa ttatcaggca gttgtgaaat cagcaataat
    14701 gagatttcat acagataggc aaacccacga ttgttgtcat acattttttt tttatctttt
    14761 gtatttttgc tgaagataat tgtctaattt tgtaagtaat taaactgacc aaactccatg
    14821 ctcaccaaac aagaacgaaa cgaaatcatt gctcttatcc ggcaggaa
//
LOCUS       DAOPYB010000061        14538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4504_length_14538_cov_10.820203, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000061 DAOPYB010000000
VERSION     DAOPYB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14538
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4504_length_14538_cov_10.820203"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PKH58_06440"
     CDS             <1..1074
                     /locus_tag="PKH58_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX88701.1"
                     /translation="VIILAATNRADILDKALLRAGRFDRQIHVDLPDVHERKEIFNVH
                     LRPIKIDNTVDVDFLARQTPGFSGADIANVCNEAALIAARKAKSFVGKQDFLDAVDRI
                     IGGLEKKNKITTVDEKKSIAYHEAGHATISWKLHYANPLVKVTIVPRGEALGAAWYLP
                     EERQLTNREHMLDEMCATLGGRAAEEVFLKQTSTGAINDLERVTKRAYAMVAYYGMSE
                     KLPNMSYYDSTGNSDYGFTKPYSEKTAETIDEEAKLIVAEQYERAKQILTENTEGHNK
                     LAELLIDQEVIFAEDVEKIFGKRPWESRSDELMRLNEEANALKEKTEETGPTAALQTG
                     DDAVQTEETADNTEPKEAEKDEQ"
     gene            1064..1726
                     /locus_tag="PKH58_06445"
     CDS             1064..1726
                     /locus_tag="PKH58_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNX88702.1"
                     /translation="MNNNSSKDNILKRIQDAKQTREFGSVFSVSTDAEIYQPLEPDAV
                     SCFKKELEAINGQCEIFETVDELFFHLKKMLNDKNIETVFCRDSHISAQLQKSDIPYT
                     QNPDDFETMKAAVTRCEFLIARTGSVVVSSAGESGRQLISYPPVHIVMAGTDQLVSYP
                     KDAYDAIQKKYINNLPSQITTITGPSRTSDIEKTLVLGAHGPKEFIILLCNDKSFNNF
                     AI"
     gene            1781..2587
                     /locus_tag="PKH58_06450"
     CDS             1781..2587
                     /locus_tag="PKH58_06450"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNX88703.1"
                     /translation="MNNFVKRSISGGIFVTIIVCSVLLSPYTFAVIFTVFAALGVHEF
                     HKLTNSTETAVNKTVAIIGAVLLFTCSFIYASGKYQYPIFSFYGFYILAVLIAELYRK
                     KRNPVNNWAYFILGQILIALPFSLLSFILYIRDYQPIILLAVFISIWVNDTGAYVTGM
                     LLGKHKLFERISPKKTWEGFIGGAVFALLSGYIFSLLIPELSLIQWLIFSEIVVIFGT
                     FGDLSESLLKRTEQVKDSGNIIPGHGGLLDRFDSMLMAAPVIFIWLSFIL"
     gene            2636..3793
                     /locus_tag="PKH58_06455"
     CDS             2636..3793
                     /locus_tag="PKH58_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX88704.1"
                     /translation="METYNFDDIRSYNTEEIPAAMERLCNQKPFMKVVSTLFPLMPKE
                     QLKQKMLSFTSSTELQLQMVYPFLQYLEANMTTGICLTGLENIDIEKKYLYISNHRDI
                     ILDSALLCAKFIEKKIDTVEIAIGDNLLVYPWIEDLVRVNRSFIVQRGLNARQVLESS
                     KRLSAYLRHTLNERKKSIWIAQREGRAKDSNDLTQESLLKMFNLAGASGSFTDNLKEL
                     NICPLSISYEFDPCDYLKAREFQLKRDNPEYKKTPEDDLINMQTGVMGYKGKVVYHIS
                     GCINDSLDIIARDTQNRNEQIEKAAQLIDYHIHKNFTITANNKIAYDEIYGDKDYAKD
                     YTTAEKISFEEYLDKQIRKIDLENKDRDFLRRKMLEMYANPLKNYLKANGL"
     gene            complement(3856..4326)
                     /locus_tag="PKH58_06460"
     CDS             complement(3856..4326)
                     /locus_tag="PKH58_06460"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HNX88705.1"
                     /translation="MKTTAVCPVSDKRINEHIARTNALFAVVFILAYVFTSNELIILL
                     LLVDFLLRGFELSAYSPFAFLSAKLVRFLNIKPKIVNAGPKLFAARIGAFFSLAILVT
                     SVAGLDVAAYILTAVFGLCASLEGFFGYCIACRIYPWFYRVFILHSVKSETNSL"
     gene            complement(4434..5270)
                     /locus_tag="PKH58_06465"
     CDS             complement(4434..5270)
                     /locus_tag="PKH58_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX88706.1"
                     /translation="MPKRFKKIYIEITNRCNYHCSFCYDTRRPAKFMSPEEFGFIVKK
                     IHPYTDYIYLHVLGEPLLHPHLAQMLQIAAETGLKVNLTTNGSLLQKQQALLIQHTVR
                     QINISLHDAEENISSDNWKEFIENTLNIACELSQTSYISFRLWNQTNPTSKDFNELCF
                     RLIIDKFQIQGNVFGQNGRNIALAPNIWLQLAPRFEWVKNEKITEKNCYGMRDQIAIL
                     SDGSVVPCCIDADASILLGNIFTDQLSDILKSERAQHIKNGFEQHKAVEHYCQTCGFS
                     VD"
     gene            5372..6604
                     /locus_tag="PKH58_06470"
     CDS             5372..6604
                     /locus_tag="PKH58_06470"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNX88707.1"
                     /translation="MWWMDLGIKAGLGAAKIASLWNKKIKQALDGRRNLPEKIATVIH
                     PGDKVIWFHAASFGEFEEGRPLLEEIHQHYPQYKILVTFFSPSGYNAHKNYPHADLVC
                     YLPADITRDVRSFLDVVHPEIAIFIKYEFWPNLLSEMKKRLIRTFVVSARFIPNSRFF
                     KWYGGVFRKALSAFETIFVQDKRSIELLKTIGYNNVVLAGDPRFDRVLGISREEWKDE
                     IVEKFKNKEKLFIAGSTVGAADEDLLQQLINRHPKTRFLLVPHEMDREPMNKIVANTY
                     RKTLIYSECTPETDFSDTQVLIVDKIGLLARLYRYGNWAFIGGGFVAGIHSVIEATIY
                     GMPAAFGPNFQKNRPGIEMVEMGVCASIRAIEELDAWFSPLEFNDEELQDISRKARNY
                     SLANCGATELILKKIFGN"
     gene            complement(6601..7764)
                     /locus_tag="PKH58_06475"
     CDS             complement(6601..7764)
                     /locus_tag="PKH58_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HNX88708.1"
                     /translation="MERRQVIAFIPVRGGSKSIPLKNIREFCGKPLVYWNISALQAVP
                     SVDKIVVATDSDIIENTVSGFGFDKVKVYRRSSENASDTASTESVMLEYIQAVGLPSE
                     TVFMLVQATSPLTQTIHFREALELYSQGNYDSLLTCVVNKRFFWNENGTSRNYDYRNR
                     PRRQNFDGELMENGAFYINTVKNILESQNRLSGRIGIYRMPEYTAIEIDEPDDWTMLE
                     NLMRKHVLSNKPSKAIKLFLTDVDGVLTDGGMYYSEAGDELKKFNTRDGMGIQLLREA
                     GIKTGIITSEMTQLVERRAAKLKMDYLYQGKNTGGKLQTALEICRKEGITLADTAYIG
                     DDINCYELLCNVGLAACPADAAQKIKSIPNIRILNRKGGEGAVREFAEIILAD"
     gene            complement(7746..8630)
                     /locus_tag="PKH58_06480"
     CDS             complement(7746..8630)
                     /locus_tag="PKH58_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNX88709.1"
                     /translation="MQKPKIIAEIGCNHKGEMEIAHEMIKTAATFCNADVVKFQKRSN
                     RELLTEEEFNAPHPNPYNSYGATYGEHREFLEFTLEQHRQLKNWCDEFGLEYSCSVWD
                     VTSAKEIISLKPSLIKVPSACNLNQELLAYICDHFEGEIHLSFGMTNRDEEEKIVTFF
                     ESKGRNNDLVIYSCTSGYPVPFEDICLLELTRLRSTFGSRVKAIGFSGHHLGIAIDAA
                     AVALGAEWVERHYTLDRTWKGTDHAASLEPDGLRKLCRDCRAVYKALKYKKEDILDIE
                     KPQLAKLKKNQIRWNDDK"
     gene            complement(8699..10021)
                     /locus_tag="PKH58_06485"
     CDS             complement(8699..10021)
                     /locus_tag="PKH58_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNX88710.1"
                     /translation="MNLFWKKMFGGMLSTSKFEKIEEAAVNDMKRYDEIGQSLELEEY
                     KKLYHVVKSSEFKEKKKTLQNRKYKDMEEYRISRKYQKLHDNSGIRQYYETLQSEQLK
                     NFLTFKSTPEYESLGDKKKVMASDKLRKLKDFENSKEYRNYIRFHDSFIIKDYEELKI
                     KISAPDFMKMNQFWADQHRWKTVPEYKIEQRYYELAKNPDIIFYEKENPVRFDKYRKL
                     KPVFIEQFEWNTLDKSRWAYGFHSANPELIGNYSFENELQANSGGKNVSVNNGVFCLE
                     TRREKCISRAWHPTKGFIEKEFDYTADVVQTADEFRQQYGVFKAKIRCTGRVHHAFWL
                     SNSSPLPHINVFHYDGKAITMGNAHKNVMDEVRVTGLKHSSFYIYTLVWTPKELIWYI
                     NDMEVYRTTSNVPKDNHLYLGFNSFLPKKGKPSTGSLEVDWVKVLAFN"
     gene            complement(10028..10576)
                     /locus_tag="PKH58_06490"
     CDS             complement(10028..10576)
                     /locus_tag="PKH58_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNX88711.1"
                     /translation="MKVCVFCSSSNNIADRYKQSAFRFGQYLAEKGMTLVFGGATGGL
                     MDAVAEGAKSGKGEIIGVIPEVIIKMNRLSALPTQQIIVNDMSERKKRLKQESDVFVV
                     LPGGFGTLDEMFDVIASGTVGEHRKPLICINEDGFYSKLLDQIERMQTECFTPKNQNY
                     KPVFVATPEECMEQISNLHTNK"
     gene            complement(10794..12761)
                     /gene="gyrB"
                     /locus_tag="PKH58_06495"
     CDS             complement(10794..12761)
                     /gene="gyrB"
                     /locus_tag="PKH58_06495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNX88712.1"
                     /translation="MNEEQIKNSAGQYGANSIQVLEGLEAVRKRPAMYIGDISLKGLH
                     HLVYEVVDNSIDEALAGYCDMIHVTINEDNSIKVTDNGRGIPVDMHEKEGKSALEVVM
                     TVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSTLLRVEVARDGRLYAQEYSCGIPKAP
                     VAEIGETDRQGTSVTFLPDDSIFTETIYKYDILASRLRELAYLNAGITLTLTDKRVQQ
                     EDGSYKKEVFYSKEGLKEFVEYIDESRENLIDQVIHISTEKNGTPVEIALLYNTSYNE
                     NIHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEDNKLLEKLEKAKIEISGDDFREGL
                     TAVISIKVAEPQFEGQTKTKLGNNEVMGSVDMAVSEALGNYLEENPKDARTIVDKVVL
                     AATARHAARKARELVQRKSPLSGGGLPGKLADCSSKDAEKCELFLVEGDSAGGTAKQG
                     RSREFQAILPLRGKILNVEKAMQHKVLDSEEIRNIYTALGVTIGTEDDSKELNLSKLR
                     YHKVIIMTDADVDGSHIATLLMTFFFRHMKEIIEQGYLFIATPPLYRCSKGKVEEYCW
                     NDQQRKAFIDKYGGGNESGIHTQRYKGLGEMNEIQLWDTTMNPDNRTLRQVTIENAAT
                     ADHVFSMLMGDDVAPRRQFIEQNATYANIDA"
     gene            complement(12969..14279)
                     /locus_tag="PKH58_06500"
     CDS             complement(12969..14279)
                     /locus_tag="PKH58_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HNX88713.1"
                     /translation="MKTEKLFNRSIFLVIGIFIMSFGVALSVKSNLGTTSISAFPYVL
                     SLFTSFSIGTYTVVLNAFFIVLQILILRKKFHPLQLFQLPVVFFFGWFIDLSVSALSG
                     FSVSAYWMEWLLSLLSSVVIALGVFLLVKSNMILLPGDALVMAIANKRKLEFGKVKIA
                     FDSLLVVISVVFSLIFLRNLQGVREGTVVAAFLVGAITRFYSQKFTFVDKLIGVTDKI
                     DITPPEEIHTQVITISREYGSGGHEVGELVAKKLGFTFYDTNLIELSALEGGFTPEFV
                     KENEQKLKNSLLYSLYKHNYAYVNEIMPPQDALFLVQSKIIRDISEKSSCVIVGRSAD
                     FILKSHPNCFNVFVHADKAFRLERIINNYGFAPGEAEAQMERKDKERINFSKYYTHRN
                     WGEPENYHLTVDSSTFGIEIAAALIVDAWRKTNVLGKTTLNPQS"
BASE COUNT         4178 a         3191 c         2917 g         4252 t
ORIGIN      
        1 gtaatcattc tggctgctac gaatcgtgcc gatattctgg acaaggcatt gctccgtgcg
       61 ggacgtttcg accgtcagat acacgtggac ctgccggatg tacacgaacg taaggaaata
      121 ttcaacgttc atttgcgtcc catcaaaata gacaataccg ttgatgttga ttttctggct
      181 cgtcagacac ccggattttc cggtgccgac atcgccaatg tctgcaacga agccgcactt
      241 atagccgcgc gaaaagccaa atcttttgtt ggcaaacagg attttctgga tgccgtagac
      301 cgtatcatcg gaggtctgga aaagaaaaac aaaatcacga ctgtggatga gaaaaaatcc
      361 attgcctatc acgaagccgg acatgccacc atcagctgga aactgcatta cgctaatccg
      421 ttggtaaaag taaccatcgt tccgcgtggt gaagccctgg gtgctgcgtg gtacttgcca
      481 gaagaacgcc agctgacaaa ccgcgagcac atgctcgacg aaatgtgtgc cacactcggc
      541 ggaagagctg cagaagaagt tttcctcaaa caaacatcga caggtgcgat taacgacctg
      601 gaaagagtta ccaaacgggc ttatgccatg gtggcttact atggaatgag tgaaaaactg
      661 cctaacatga gctattacga ctcaaccgga aattctgatt acggatttac aaaaccgtac
      721 agcgaaaaaa cagccgaaac aatcgatgag gaagccaaat taatcgttgc cgaacagtat
      781 gaacgcgcaa aacagatact tacagaaaat accgaaggac acaacaaact ggctgaactt
      841 ctgattgatc aggaagttat ctttgccgaa gatgtcgaga aaatctttgg aaaacgtccg
      901 tgggaatctc gcagcgatga gttgatgagg ctgaacgaag aggctaacgc gctgaaggaa
      961 aaaacagaag aaacaggacc aacagcagca cttcaaacgg gtgacgatgc agttcaaacc
     1021 gaagaaactg cagataacac agaaccaaaa gaagctgaaa aagatgaaca ataattcttc
     1081 caaagataat attctgaaaa gaatacagga tgcaaaacag acccgtgaat tcgggtctgt
     1141 tttttccgtt tcgaccgatg ctgaaatcta tcagcccttg gaaccggatg ctgtaagttg
     1201 ctttaagaaa gaactggaag cgataaacgg ccaatgcgag atttttgaaa ctgtggatga
     1261 actttttttt catctcaaaa agatgttgaa tgataaaaac attgaaacgg ttttttgcag
     1321 agacagccac atcagcgctc aactccagaa aagtgatatt ccctacactc aaaatccgga
     1381 cgattttgaa acgatgaaag cagcagttac acggtgtgag ttcctcatag cccgtacagg
     1441 cagcgtggtg gtctcttctg cgggagaatc cggacgtcag cttatttctt atccacctgt
     1501 tcatatcgtt atggctggta ccgatcagtt ggtcagctat ccgaaagatg cctatgacgc
     1561 catacagaaa aagtatatta acaaccttcc atcacaaatc acaacaataa cagggcccag
     1621 ccgcacttcc gacatcgaaa aaacgcttgt tttgggcgct cacggaccga aagaatttat
     1681 tattttgctt tgcaatgata aatcttttaa taactttgca atataaatcc gactacgggg
     1741 acaaaacctg tatatccgta atgctgcaaa ataaaggtgt atgaataatt tcgtgaaacg
     1801 atccatcagc gggggcattt ttgttaccat aatcgtatgt tccgttttat tgtcacccta
     1861 tacttttgca gttattttta ctgtattcgc agcacttggt gttcacgaat ttcacaaact
     1921 gactaactcc accgaaactg ccgtaaacaa aactgttgca attatcggag cggtactctt
     1981 gtttacctgc tcatttatct acgcctccgg caaataccag tatccgatat tttcattcta
     2041 cggattttat atcctggccg ttctgattgc ggaattgtac cggaagaaac gaaatccggt
     2101 gaataactgg gcttatttta tccttggaca aattttgatt gcattaccct tctcattact
     2161 cagtttcatt ttatacatca gagattatca gcccatcata ctgcttgccg tttttatttc
     2221 catctgggtc aacgataccg gagcatatgt aaccggaatg ttactgggca aacataaact
     2281 tttcgaacgg atttccccca aaaaaacctg ggaaggtttc atcggcggag ccgtttttgc
     2341 tttattatcg ggctatatat tttcactttt aataccagaa ctcagtctta ttcaatggct
     2401 catattttcc gaaatcgtcg ttatcttcgg cacctttggc gacttgtctg aatcgctgct
     2461 taaacgcact gaacaagtaa aagattcagg taatattata cccggacatg gtggacttct
     2521 tgatcggttt gacagtatgc tgatggcggc gcctgtaata tttatctggt tgagttttat
     2581 tttataagct gataataaaa tactttttat aaacgaacat taatacagca ttggtatgga
     2641 aacttataat tttgatgata tacgctctta caatacagaa gaaataccgg ctgccatgga
     2701 gcgtctgtgc aaccagaaac ctttcatgaa ggtagtgagt acacttttcc cgcttatgcc
     2761 gaaagaacag ctgaaacaga agatgctgag tttcacctcc tccaccgaac ttcaacttca
     2821 gatggtatat ccctttcttc aatacctgga agccaacatg accaccggta tctgcctgac
     2881 gggactggaa aacatcgaca tcgaaaaaaa atatctgtac atttccaatc atcgggatat
     2941 tatattggat tcagctcttt tatgtgcaaa attcattgag aaaaaaattg ataccgtaga
     3001 aatagccatc ggagataacc tcttggtata tccatggatt gaagatttag ttagagtaaa
     3061 cagaagtttt attgtacaac gcggactgaa tgcccggcag gtactggaaa gttcgaaacg
     3121 cctttcggct tacctgcgtc acacactgaa cgaaaggaaa aaatcaatct ggatagccca
     3181 gcgcgaaggc cgggccaaag actccaacga tctgacacag gaaagcctgt tgaaaatgtt
     3241 taatcttgct ggcgcatccg gctcgtttac cgataatctg aaagaactga acatctgtcc
     3301 gttaagtatc tcttacgaat ttgatccgtg cgattatctg aaagccagag aattccagct
     3361 taaacgtgat aatccggagt acaaaaaaac gcccgaggac gatctgatca atatgcaaac
     3421 cggagtgatg ggctacaaag gtaaagtggt gtatcatatt tccggatgca tcaacgacag
     3481 tctggatatt attgcccggg atacacaaaa cagaaacgaa cagatagaaa aagcagcgca
     3541 attaattgac tatcatatac ataaaaattt caccattaca gcaaacaata aaatagccta
     3601 tgatgagatt tatggagata aagattatgc aaaagattat actactgccg aaaaaataag
     3661 ttttgaggaa tatctggata aacaaatccg gaaaattgac cttgaaaaca aagatcgcga
     3721 tttcctgcgc cggaagatgc tcgaaatgta tgccaatccg ctgaaaaact atttaaaagc
     3781 caacggcctg taaatagacg ctcgtaacat ataaaaggtt cagaaagtca atactttctg
     3841 aacctttttt gttttttata gagaattcgt ttcgctctta acggagtgca ggataaaaac
     3901 acggtaaaac cagggataaa tccggcaggc aatacaatag ccaaaaaatc cttcgagcga
     3961 agcacacaaa ccaaaaactg cggtcagtat gtatgcggca acatccagtc ctgctaccga
     4021 agtgaccaaa attgccagac tgaagaaagc acctatccgt gctgcaaata gcttgggacc
     4081 ggcgttgact attttaggct ttatattcag aaacctgaca agttttgccg aaaggaaagc
     4141 aaacggacta tatgctgata actcaaaacc acgtaacaaa aagtcgacga gcagaagtaa
     4201 tatgattaac tcattggatg taaacacata tgccaatata aatacgacag cgaacaaagc
     4261 atttgttcgt gcaatatgtt cattaatcct cttatccgaa accggacaaa cagcagttgt
     4321 tttcattttc ttatttatta aaattacgaa acaaagatac aactgcaaaa tactgccatc
     4381 aatcccctga tggaggtata ttctataaca tcaatgtggg ataatacttc tgattaatca
     4441 acgctgaaac cgcaggtctg acaatagtgt tcaaccgctt tgtgctgctc aaaaccattt
     4501 ttgatatgct gagcacgttc cgatttcaga atatccgata gctgatccgt aaaaatattt
     4561 cctagcagaa tactggcatc cgcatcgatg caacaaggca ccacacttcc gtcagataga
     4621 atagcaatct gatctcgcat gccgtagcaa ttcttctcgg tgattttttc atttttaacc
     4681 cattcgaaac ggggtgccag ttgcagccag atattgggag ccaaagctat atttcgtccg
     4741 ttttgaccga aaacatttcc ctgaatttga aatttgtcta ttatcaatct gaaacataat
     4801 tcattaaaat ccttacttgt cgggttcgtt tgattccaga gacgaaaact gatataggag
     4861 gtctgggaca gctcgcatgc aatattgagg gtattttcaa taaattcctt ccagttgtca
     4921 gatgagatat tttcttcggc atcgtgcaaa gatatgttta tctgtctgac tgtatgttgt
     4981 atgagcagtg cctgttgttt ctgcagtaag cttccgttgg tggtcaggtt aactttcaat
     5041 ccggtttcgg cagctatttg caacatttgc gccaagtgcg gatgcagcag tggttcacca
     5101 agcacatgca gatatatgta atcggtataa ggatgtattt ttttcacaat aaatccaaac
     5161 tcttccggcg acataaactt tgcgggtcgc cgggtatcat aacaaaaact gcaatgataa
     5221 ttacaccggt tggttatctc aatgtatatt ttcttaaatc tttttggcat gatgatgatt
     5281 gatttcatcg gcaaagtaag gcaaatcatc aaaactgacc aaattaattc ttaaattcgt
     5341 atctttgcat aaaaaaatca gtaacttgca tatgtggtgg atggatcttg gcataaaagc
     5401 cggtctgggc gcagcaaaaa ttgcttcgct gtggaataag aaaattaaac aggcactgga
     5461 tggccggcga aacctgccgg aaaaaattgc tacggtaata catcccggcg acaaggtgat
     5521 atggtttcat gccgcttcgt ttggagagtt tgaagaagga cgccctctgc tcgaagaaat
     5581 acatcagcac tatccgcaat acaaaatcct ggttacattt ttctctccat ctggatacaa
     5641 cgcgcataaa aattacccgc atgctgatct ggtatgttat cttcccgcag atatcacccg
     5701 cgatgttcgc agttttctgg atgtggttca tcccgaaatt gccattttta tcaagtatga
     5761 attctggccg aacctgctgt ccgaaatgaa aaagcgttta atccgcacct ttgtggtttc
     5821 agcccgcttt atccccaatt cacggttttt caaatggtac ggaggcgttt tccgcaaggc
     5881 tttgagcgct ttcgaaacca tttttgttca ggataagcgt tccatcgaac tgctgaaaac
     5941 aatcggatat aacaatgtcg tcttggccgg tgatccgcgc tttgaccgag tgctgggcat
     6001 ctcacgtgaa gagtggaaag acgaaatcgt cgaaaaattc aaaaacaaag agaaactttt
     6061 catcgccggt agtacggtag gcgcagcaga tgaagattta ctacagcaac tcatcaacag
     6121 acatccgaaa acacggtttc tgctagtacc tcacgagatg gacagggaac cgatgaacaa
     6181 aattgtagcc aatacttacc ggaaaacact gatttactct gaatgtacgc ccgaaaccga
     6241 tttttccgac acacaggttc tgattgtgga taaaataggt ctgctggcaa gactttaccg
     6301 ctacggcaac tgggcattta tcggtggtgg gtttgtggcc ggcattcaca gtgtgataga
     6361 agctactatt tacggaatgc ccgcagcatt tggtccaaac tttcagaaaa atcgtcccgg
     6421 aattgagatg gtggaaatgg gtgtttgcgc gtcaatcaga gctattgaag aactcgacgc
     6481 gtggttttct ccgctcgaat ttaacgacga agaattacag gatatcagcc ggaaagcccg
     6541 gaattattca ctcgctaact gcggtgcaac cgaactgatc ctgaaaaaaa tcttcggcaa
     6601 ttaatcagca aggattattt cggcaaattc tctgactgca ccctcgccgc cttttcggtt
     6661 aagaattctg atgttcggaa ttgattttat cttttgggca gcatctgccg gacatgcagc
     6721 caatcccaca ttacaaagca attcgtaaca gttaatatca tcgccgatgt aggctgtatc
     6781 agctaaggta attccttctt tccggcagat ttctaatgct gtttgtaatt ttccaccggt
     6841 atttttcccc tggtataaat aatccatttt cagcttagca gcccgtcgtt ctacgagttg
     6901 ggtcatttcg gaagtaatta tacccgtttt aattccagct tcacgaagca actgtatgcc
     6961 catgccatcg cgggtattga atttcttcag ttcatcaccg gcctcggaat aatacatgcc
     7021 accgtcggtc agcacaccat ccacatcagt cagaaagagt ttaatcgcct tggaaggctt
     7081 gttactcaat acatgtttgc gcatcagatt ttccaacatg gtccagtcgt cgggttcgtc
     7141 gatttcgatg gctgtatatt cgggcatcct gtaaatgcca atcctaccac ttaaacggtt
     7201 ttgtgactct aggatattct ttacagtgtt gatataaaat gcaccatttt ccatcaactc
     7261 accatcgaaa ttctggcgcc gtggacgatt tcggtaatca tagttgcggg atgttccgtt
     7321 ttcgttccaa aaaaagcgtt tgtttacgac gcaggtaagc aacgagtcgt aatttccctg
     7381 cgaataaagt tcgagggctt cccggaaatg aatggtctga gtaagcggtg acgtagcctg
     7441 aacaagcata aagactgtct cagagggcaa accaactgcc tgtatgtatt cgagcataac
     7501 gctttcggta ctcgcggtat ccgaagcatt ctctgacgaa cggcgataaa ctttcacttt
     7561 gtcaaatcca aacccgctga ccgtattttc gattatgtcg gaatcagtgg caacaacgat
     7621 tttatcaacg gaaggcacag cctgaagcgc cgatatattc cagtaaacca gcggttttcc
     7681 gcaaaattcg cgaatattct taagcggtat tgatttactg cctcctctca ccggaataaa
     7741 agcaatcact tgtcgtcgtt ccatcgaatc tgatttttct taagtttagc gagttgtggc
     7801 ttttctatat ccagaatatc ttcttttttg tatttcaatg ctttataaac ggctctgcaa
     7861 tcgcggcaca gtttacgcaa accgtcaggt tcgagtgatg cagcatgatc agtgcctttc
     7921 caggtgcggt cgagtgtata atgacgctca acccattcgg cgcccaaagc tacagctgcc
     7981 gcatctatcg cgatgcccag gtgatgaccc gaaaagccaa tggcttttac tctgcttcca
     8041 aaagtggatc tcaaccttgt aagttccagc agacaaatat cttcgaaagg aaccggataa
     8101 cccgaagtac agctataaat taccagatcg ttgttacgac ctttgctttc aaaaaaagta
     8161 acaatttttt cctcttcgtc gcggtttgtc attccgaatg aaagatgaat ttcaccttcg
     8221 aaatgatcac aaatataagc aagcaattcc tgattgaggt tgcaggcaga cggtactttt
     8281 attaaggaag gcttcagaga gatgatttct tttgctgaag ttacatccca taccgaacag
     8341 gagtattcca gtccgaattc gtcgcaccag tttttcagct gacgatgttg ttccagcgta
     8401 aactccagga attcgcggtg ttcgccgtaa gttgctccgt atgaattata cggattgggg
     8461 tgaggagcat tgaattcttc ttcggtgaga agttcgcggt tacttcgttt ctgaaatttc
     8521 accacatccg cgttacaaaa tgttgctgca gtttttatca tttcatgggc aatttccatt
     8581 tcccccttat gattacaacc gatttcagcg attatctttg gtttctgcat gaattttttt
     8641 cgttatcact cccgctatcc ggatgtttat ttcaggatac gaggaattaa atttcttatt
     8701 aattaaacgc cagcaccttt acccagtcaa cttcgagcga gcctgtcgaa ggtttccctt
     8761 ttttgggcag aaacgaattg aatcccagat aaagatggtt gtctttaggt acattggagg
     8821 tggtacgata tacttccata tcgttgatgt accagatcag ttcctttggt gtccacacga
     8881 gggtatagat gtagaaagaa ctgtgtttca gacctgttac cctcacttcg tccatcacat
     8941 ttttatgagc attgcccata gtgattgcct ttccgtcgta atggaaaaca ttgatatgcg
     9001 gaagcggaga actgttacta agccagaaag catgatgaac acgaccggtg cagcgtatct
     9061 ttgctttgaa tactccgtat tgctgacgga attcatcggc agtttgcacc acgtcagctg
     9121 tatagtcaaa ttccttttcg ataaatcctt tggtgggatg ccaggcacgc gaaatgcatt
     9181 tttcacgccg tgtttcgagg caaaacaccc cattattgac actgacattc ttgccgccac
     9241 tatttgcctg cagctcgttt tcgaacgaat agttaccaat cagttccgga ttggcagaat
     9301 gaaaaccgta tgcccagcgc gatttatcga gtgtattcca ttcaaattgt tcgataaaga
     9361 ccggtttcag ttttctgtat ttatcgaaac gtacgggatt ttctttttcg tagaaaataa
     9421 tatcgggatt ttttgccagt tcataataac gctgttctat tttatactcc ggcactgttt
     9481 tccagcgatg ctgatcagcc cagaactggt tcattttcat aaaatcagga gctgaaattt
     9541 ttattttcaa ttcctcgtaa tctttgatga tgaatgagtc atgaaaacgg atataattcc
     9601 tgtattcttt cgaattttca aagtctttga gcttcctaag cttatcggaa gccataacct
     9661 tctttttatc acccagactt tcgtattccg gagtcgattt aaatgtaagg aaattcttta
     9721 gttgctccga ttgcagtgtt tcgtaatact gacggatacc actgttatcg tgtaactttt
     9781 gatattttcg gctgatacgg tattcctcca tatccttata ttttctgttc tggagtgttt
     9841 tctttttctc tttgaattcg gaagatttca ctacatgata gagttttttg tactcttcga
     9901 gttcgagcga ctgaccgatt tcgtcgtagc gtttcatatc attgacagct gcttcttcga
     9961 ttttttcaaa tttcgatgtg gaaagcattc caccgaacat ttttttccag aatagattca
    10021 taatattcta tttgtttgta tgcagattac tgatttgttc catacattct tcgggtgtag
    10081 caacaaaaac aggtttgtaa ttttgatttt tcggagtgaa acattccgtt tgcattcttt
    10141 caatctgatc aagcaatttt gagtaaaatc catcttcatt gatacatatc agcggtttcc
    10201 ggtgttcgcc tacagtgccc gaagcaatga catcaaacat ttcgtcgagt gttccaaatc
    10261 cgccgggcag gacaacaaat acgtcggatt cctgtttcag ccttttttta cgttcactca
    10321 tgtcatttac aatgatttgt tgagttggca gcgcgcttag ccggttcatc ttgattataa
    10381 cttcgggtat caccccgatt atctcccctt ttcccgattt tgcaccttcg gccacagcat
    10441 ccatcaggcc acccgttgct ccgccaaaaa cgagtgtcat tcctttttct gccagatact
    10501 ggccaaaccg gaaagccgat tgcttgtaac ggtcagcaat gttattactt gatgaacaga
    10561 agacacagac tttcatgaat tcaactgtta taaatttttg aatatgaaaa aaatatatgc
    10621 agtttttaat aaaatgatta ttaaccggaa actgtgaatc gtacgtcacc gttctgattt
    10681 tctgataaaa aacgtagatt tttgtataat cttataccgc aacgtgattt tacagaaaat
    10741 tgaatatccg atcagtacag atattcaatt ttcacaattt cacttatcag cttttatgca
    10801 tcaatatttg cataagtcgc attctgctca ataaactgtc ttcttggcgc cacatcatcg
    10861 cccattagca tagagaaaac gtggtcggca gtggctgcat tctcgatggt tacctgacgc
    10921 agggttcggt tatccgggtt cattgttgta tcccagagct gtatttcatt catttctccc
    10981 aaacctttgt aacgctgcgt atggatacca ctttcatttc cgccaccgta tttgtctata
    11041 aaagctttac gctgctggtc gttccagcag tattcttcaa ctttgccttt ggagcaccgg
    11101 taaagcggtg gcgttgcaat aaagagataa ccctgctcga taatttcctt catatgtcgg
    11161 aagaagaatg tcatcagcag tgtggcaatg tggcttccgt ccacgtcggc atcggtcatg
    11221 attataactt tatgatagcg cagtttggag aggttcagct ccttgctgtc gtcctctgtt
    11281 ccaatggtta caccgagagc cgtgtaaata ttacggattt cttcgctgtc aagcactttg
    11341 tgctgcatgg ctttttccac attcagaatt ttacccctca agggcaaaat tgcctgaaat
    11401 tcgcggctgc gtccctgttt ggctgtacca ccggcagagt ccccttcgac gaggaacaat
    11461 tcacatttct cagcatcctt actggagcag tcggccagtt tacccggcaa tccgccaccc
    11521 gaaaggggag atttgcgttg tacaagttca cgggctttgc gggcggcatg acgtgctgta
    11581 gctgccagaa ccactttatc aacgatggtg cgggcatctt tcggattttc ttcaaggtaa
    11641 ttgccaaggg cttcgcttac agccatatcc accgaaccca ttacttcgtt attaccaagt
    11701 ttggttttgg tttgaccttc aaactgcggc tcagccactt tgatggagat aacagctgtc
    11761 agtccttctc gaaagtcgtc accgctgatt tctattttag ctttttcgag tttttcgagt
    11821 aatttgttat cttcggcata ttttttcagt gtacgggtaa gtccgcgtct gaaacctgcc
    11881 aagtgagtac ctccttcaat ggtattgata ttattcacat aagaatggat attttcattg
    11941 tatgaggtat tgtaaagaag tgcaatttca acgggcgtac catttttttc ggtcgaaatg
    12001 tgtattacct gatcaatgag gttttcacga ctttcatcaa tatattcgac aaattccttc
    12061 agaccttctt ttgaatagaa aacttctttc ttgtagcttc catcttcctg ctgtactctc
    12121 ttatcggtca gcgtgagggt tattccggca ttcagatatg ccagctcacg aagccggctt
    12181 gccagaatat catatttata aattgtttcg gtaaaaatac tgtcatctgg caggaatgtc
    12241 accgaagttc cctgcctatc ggtttcaccg atttcggcaa ccggcgcttt ggggataccg
    12301 caggagtatt cctgagcata caaccttcca tcgcgggcta cttccacacg caacaacgtt
    12361 gaaagtgcat tcacgcatga cacacccact ccatgcaatc cgcctgaaac tttataactt
    12421 cccttgtcga atttaccacc ggcatgaagt acggtcatta ccacttcaag tgcagacttt
    12481 ccttcttttt cgtgcatatc caccgggata ccacgaccat tgtctgttac ttttattgaa
    12541 ttatcctcgt tgattgttac atgaatcatg tcacaataac cggccagggc ttcatcgata
    12601 gaattatcaa ccacctcgta aaccagatga tgcaatcctt tcaagctaat atcaccaata
    12661 tacatagccg gacgcttacg caccgcttcc aaaccttcaa gcacctgaat actgttagca
    12721 ccgtactgtc cggcagaatt tttaatttgc tcttcgttca tttttatatc taatttattt
    12781 tcagactttt atatgattat tgagtatgaa atataatttt caaatttcat atctaaaaac
    12841 atacaaatat acgataaaaa tttggataac tgaagtaaaa atcgtgtcga tttttactaa
    12901 ttgcggccgt gaactcatgt gcatccgcac aggtttacac ggcagtaaaa cctgaaacca
    12961 gctttggttt agctctgggg gttaagtgtt gtttttccca gcacgttggt ttttcgccat
    13021 gcgtcaacaa tcaaggcagc tgctatttcg ataccaaaag tcgagctatc taccgtgaga
    13081 tggtaatttt ccggctcgcc ccagttgcgg tgagtatagt atttgctgaa gttaatccgt
    13141 tccttgtctt ttcgctccat ttgtgcttct gcttcgccgg gagcgaaacc atagttattg
    13201 atgatgcgtt ccagacggaa agctttatcg gcatgcacaa aaacattgaa acaattcgga
    13261 tgacttttca gtataaaatc ggccgaacgg cccacgatca cgcaggatga tttttcactg
    13321 atatcccgga tgattttact ttgaacaagg aaaagtgcat cctgaggcgg cataatctcg
    13381 ttgacatatg cgtaattgtg tttgtaaagc gagtagagca gactattctt cagcttctgt
    13441 tcattttcct ttacaaattc aggagtaaag ccaccttcga gtgcggaaag ttctatcagg
    13501 tttgtgtcat aaaatgtaaa acccagtttt ttagcaacaa gctcccctac ctcatgcccg
    13561 ccactgccat actcccggga tatagttatc acctgagtat gtatttcttc cggaggtgta
    13621 atgtcaattt tatcggtaac gccaatcagt ttatctacaa acgtaaattt ttgggaataa
    13681 aatcgggtta tcgcacccac cagaaacgcc gctaccacag taccttcacg cacaccctga
    13741 agatttcgaa gaaaaatcaa tgagaaaact acagaaataa ccacaagcag gctatcaaaa
    13801 gcaatcttca cctttccaaa ttccagcttg cgtttattgg caattgccat tacaagggca
    13861 tcacccggca ataaaatcat gttcgatttt accagcagaa aaacacctaa tgcaatcacc
    13921 acgctgctca gcaaactcag caaccattcc atccagtagg cagagactga gaaaccagac
    13981 aatgccgaca ccgacagatc aatgaaccat ccgaaaaaaa acaccacagg caactggaat
    14041 agttgtaagg gatgaaattt ttttctcagg atgagtatct gcaggacaat aaaaaaagca
    14101 ttaagaacta ctgtatatgt gcctatggaa aacgaagtaa acaaacttaa tacgtaaggg
    14161 aaagccgata ttgaggtagt tccgagatta gatttcaccg acaatgccac cccgaatgac
    14221 ataataaata ttccgattac aaggaatatc gatcgattaa atagtttttc cgttttcatt
    14281 gtccgcgtac caacagattt attcagattt gatcagtctg attttcgaca cacaaaatta
    14341 atcattttgc ttcatataaa aatgtataag acacagttaa ttacctaaaa ttaagattta
    14401 cacaccactc taaaaataac agcaattttc aatgaccgga ttttattaat ttatcagtat
    14461 tttcacaacc aaattacgac ataatgtgtt atttatttgt gtaataactg tgctgtactc
    14521 aatgaatatc tttattat
//
LOCUS       DAOPYB010000062        14414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4571_length_14414_cov_8.787659, whole genome shotgun sequence.
ACCESSION   DAOPYB010000062 DAOPYB010000000
VERSION     DAOPYB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14414
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_14414_cov_8.787659"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1033
                     /locus_tag="PKH58_06505"
     CDS             350..1033
                     /locus_tag="PKH58_06505"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX88714.1"
                     /translation="MDNIEKRKIYYTLFFPVLISLFIVLVFLFERGMGWDFYKAGIFP
                     HDLKTLPNIFTMPFIHADLSHLLNNIIPFFVLSSCLYYFYSQIANKILLLSLILTGFI
                     LWVIGRDNWHIGASGLVYSLSFFLFFSGLFRKHIPLIAISFIVVFLYGNNVWHLFPWG
                     KMDPVSWEGHLAGGITGLFLAFIYRKQGPQKPEKIWDDEDVDDMEDMEDAEIQDDYKD
                     EKDDALSEN"
     gene            1051..2016
                     /locus_tag="PKH58_06510"
     CDS             1051..2016
                     /locus_tag="PKH58_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88715.1"
                     /translation="METKKTNEINLMDVIIETGKWLKKTVVQLLEFSGKILILLYKHK
                     IITACILILSIAVGQYKSRPSARKYNMEGMCVLYGPEAKTISEIGKQLAYSSPRFQAT
                     SLARKLSLPDSIASKVTGVEFFQVIDYQLNHTPDLIDFKNSHSMEDTLNLPMIDYIYM
                     RIKIIGTSNANAIGDAMLKYINTNPTVQNQYEILKGQLNEKIRLCDAEMLRLDSLAKI
                     TYFEDKKPEIKFESNKLLIGNNTIQLFYNDMLTLQTYKANAQNRLLDAKQPVVVPSGF
                     VVNPKAINGRAKYGIYSLIIGLLLSIFVSFFLENRKKWAKYLQNN"
     gene            complement(2072..>2647)
                     /locus_tag="PKH58_06515"
     CDS             complement(2072..>2647)
                     /locus_tag="PKH58_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX88716.1"
                     /translation="VLFDEIEKAHPDVFNTLLQVLDDGRLTDNKGRTVNFKNTIIIMT
                     SNVGSSLIRENFEHINIRNRTEVIEKTKNEVFELLKKSVRPEFLNRIDEIIMFAPLDE
                     SQIMEIVKMQLYAVKNMLAENGINLKITDRAMEFISAEGYDPQFGARPVKRAIQKYVL
                     NDLSKELISGKVNSEKPVLVDCVDGRIVFGN"
     assembly_gap    2648..2747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2748..4401)
                     /locus_tag="PKH58_06520"
     CDS             complement(<2748..4401)
                     /locus_tag="PKH58_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNX88717.1"
                     /translation="MNFSNFTTKSQEVVQNAIDLAQSYNQQSIETTHLMKALLNKGED
                     VIQFLFGKMGIYPSVIQQVIDRTIESFPKVTGGEPYLSRETNDVLRKASEISASAGDQ
                     FVSLENILLALLKVKNSVSQILKDAGVNEKALEAAISELRKGAGANSASAEETYQSLD
                     RYAINLNERARSGKLDPVIGRDEEIRRVLQVLSRRTKNNPILIGEPGTGKTAIAEGLA
                     QRIVRGDVPENLKTKQVYSLDMGALIAGAKYKGEFEERLKSVVNEVIHSDGEIILFID
                     EIHTLIGAGKSEGAMDAANILKPALARGELRAVGATTLDEYQKYFEKDKALERRFQIV
                     MIDEPNIASSISILRGLKEKYENHHKVRIKDDAIIAAVELSNRYISDRFLPDKAIDLM
                     DEAAAKLRMEVNSVPEELDKIERNIKQLEIEREAIKRENDVRKLEQLEEEIANLKEDS
                     KKYRARWSSEKDIIEKIQQSKIEIENLKYEADRAEREGDYGRVAEIRYGKIKSEENLI
                     SENQSRLAEIQGNNAMIKEEVDSQDIADVVSRWTGIPVTKMLQ"
     gene            4662..5312
                     /locus_tag="PKH58_06525"
     CDS             4662..5312
                     /locus_tag="PKH58_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNX88718.1"
                     /translation="MQKIDHNIPEPTLRRLPWYLAYAKLVQHSGEPFLSSTQIATNIA
                     VDASKVAKDLSYVQISGRTRVGYEVNELVKVLESFLGFTNTHKAFLFGVGSLGGALLH
                     DDGMKQFGLEIVAGFDVKYELAGTSINRVPIHHIDHFRELQKQTLVNIGVLTVPVDKA
                     QETAEMMIDGGIQAIWNFTPFRIRVPDNIIVQNTSIYAHLAVMFNRLNELNSKIQE"
     gene            5312..5923
                     /locus_tag="PKH58_06530"
     CDS             5312..5923
                     /locus_tag="PKH58_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNX88719.1"
                     /translation="MKIFAIGQNYAEHNRELNSPDPKEPVIFMKPDTAALKNNKPFYI
                     PDFSSEVHYETELIIKISRLGKNIAPKFAYRYYEEIGLGVDFTARDIQRKLKSEGKPW
                     EICKAFDNSAVIGSFLPKEEFADIQCIDFHLNINGNPVQHGNTADMIFPVDEIIAYIS
                     RYFTLKIGDILFTGTPVGVGKVNIGDHLEGFIQDKKMFDFKVK"
     gene            6018..>8890
                     /gene="porU"
                     /locus_tag="PKH58_06535"
     CDS             6018..>8890
                     /gene="porU"
                     /locus_tag="PKH58_06535"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNX88720.1"
                     /translation="MKYNFFALFLNIIPFLTLSAQNTTIEKHVINWTGIETWQAEKSS
                     KNVLSFTGAQYPLENDLPYFVETLVWDKNYSCKVQIENQVYADLTIPEINVLENNDIP
                     QEVEVQTFISQEKGNSYPSVRLLPLIMSDGKVKKLISFDLKIEKSSKPLKAPSSVHSY
                     VNTSVLASGKFVKIKIKDSGIYKLTYEALSSMGVNPANVRVFGYGGALQEQNFLMPKP
                     DDLPEVAIWMEKGSDGVFNAGDYILFYGQGIVKWKYDSSKEMFVHTLNNYSTDAYYFV
                     TSDAGTGKKITEQTIEVPENTIQETVTEFEDYQVYELEKQNLAQTGKVFYGETFSDVL
                     SYNFPFSFPNVIKTATTKARLDVAAISSENTSFVLKLNDTQQQTVTVLKKSIDNYEVG
                     KDANVVFTYTPSADNLIFNLSYTKATPVSRGYLNYLEVNARRSLTMAGAVMFFRNTDY
                     LNFGTYNRYQLSGAGPNVQIWDVTDPLNIKKIVTTRTNNVLEFVDTNSMLKEYVAIDA
                     TSAAAFQQPSVVGEVPNQNLHGIQNAEMVILTHPTFVSQAEKLAQAHRDKDNMTIAVV
                     TTEQVYNEFSSGTPDATAYRWVMKMLYDRAMASGNASLAPKYLLLFGRGSFDNRGLIL
                     NSGNNLILTYQADMSLDVVKSYVTDDYFGFLDDNEGLQISSHLLDVGIGRFPVVTTQQ
                     AEDVVNKTIHYMENKEKGNWKNQICFLADDGDGALHMRQADSIVSLVSRNNPGFQYNK
                     IYLDSYLQEVAASGESYPLAKTKFHNMLSSGVFLLDYTGHASQLGWTNEQILTTADIK
                     EMYNTRLPFWIGATCDFILFDVKEISAGEHVLLNPAGGGIGLFSAARTVYASQNEKLN
                     RYLTVNLFNKQNGKYNTLGDAVMKSKNQVGGEINKLSYTLLADPALRLNYPTNYKVIT
                     AKINNQPVQPTDTLRALSVNSVEGYIADDNGNKISGFNGNLEVT"
     assembly_gap    8891..8990
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8991..9542
                     /locus_tag="PKH58_06540"
     CDS             <8991..9542
                     /locus_tag="PKH58_06540"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX88721.1"
                     /translation="SDIHGVNTVGSGIGHDLRLVIDDNPNTSYILNDYYEAETNSFNS
                     GSVRYKLPVMSKGKHTLTFHAWDLLNNSSQKTLEFEVVPGLTPVIFNVYNYPNPVKSQ
                     TQFVVEHDRPETILETTVELFDMMGRKIWSLKQNSVENLKWNREDTSLSHVKPGIYIY
                     KISIKTANSELTSKANKIIVTGQ"
     gene            9703..10890
                     /gene="porV"
                     /locus_tag="PKH58_06545"
     CDS             9703..10890
                     /gene="porV"
                     /locus_tag="PKH58_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNX88722.1"
                     /translation="MKKTNLILFSLLLITLSSFAQTSVNDQLNPLITGVPSLTITPDA
                     RAGGMGDVGAATTPDMNSQYWNPAKYSFMTSPAGLSFSFTPWLRSLGINDINLSYLTG
                     YWKFDDMQSVSGSLRYFSLGSVRLTDEQGNDLNMDAQPNELALDVSYSRLLSDNWSAA
                     VGFRYIRSDLNNGANGAGGIEMFPGNAFAADVATYYKTPIPMATGDANLAFGLNISNI
                     GSKISYDLNETSIFIPTNLRLGGSFEYPFDDYNKISFSADVNKLLVPTPVKPIGDETY
                     ESQSYIDRKNVYDQTGPIAGIFKSFNDAPGGMSEELKEIMWSAGAEYAYNNQFFVRGG
                     YFNENAMKGNRKYFTAGAGFKLNVFQLDVAYLISAAQSNPLDQTLRFSLAFDLNGLTD
                     LMR"
     gene            11010..11489
                     /gene="ispF"
                     /locus_tag="PKH58_06550"
     CDS             11010..11489
                     /gene="ispF"
                     /locus_tag="PKH58_06550"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNX88723.1"
                     /translation="MKIRVGFGYDVHAFAPERELWLGGVKIEHNLGLLGHSDADVLIH
                     ALCDALLGAANMRDIGYHFPDTSIHYKNIDSKLLLMETMLLIRGKGYEFVNADCTVCA
                     EQPKLNPHVPSMQQTLSGVMGVDEDDISIKATTSEKMGFVGRQEGIAAYATVLIQKQ"
     gene            11597..12223
                     /locus_tag="PKH58_06555"
     CDS             11597..12223
                     /locus_tag="PKH58_06555"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX88724.1"
                     /translation="MSVLVGKKAPAVAGKAVVNGNEIVDNFSLENYRGKYVVFFFYPA
                     DFTFVCPTELIAFQERIAEFESRNTAVIGASTDSEFSHWKWLQTPQNQGGIQGVKYPI
                     YADQNMTVSKAYDVLAGYEEYTEEGELVFVGAPQAYRGLFLIDKEGVVRHQVVNDMPL
                     GRSVDEVIRVLDALQFTEEYGEVCPANWKKGDKALKPTQEGISDYLSH"
     gene            12370..13131
                     /locus_tag="PKH58_06560"
     CDS             12370..13131
                     /locus_tag="PKH58_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNX88725.1"
                     /translation="MDMTWLYALVSTLVVSLISLIGVFLLSFKAQQLHRVLILLVCFS
                     IGAMLGNTFFELLPESFLHNSDASLTGWLAIGGFLVFFVLEQFLHMHQHSHSKGKGYI
                     KSYGYLSLYADSIHNFTDGILIGAAWMFTPEIGLATTFAILLHEIPQEISDFGILLQA
                     GFSKRKALLNNFYAACTAILGTIITLAVGEEVEHLSVYILPVAAGGFIYLACSSLLPE
                     ILKETTKKNYLIHLLFILLGLYLMYFFSIHGGHQH"
     gene            13136..14242
                     /locus_tag="PKH58_06565"
     CDS             13136..14242
                     /locus_tag="PKH58_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88726.1"
                     /translation="MKKYLISIILFITGLSISTSAQELQWNLNLNYFFDNTEFARSAL
                     SNDQTMTGVHFSPELGMKWDENNSIYGGVDLLKKSASKTFADNVKFIAYYQFRNENTL
                     FKAGAFQRGDLLSDYSTAFFQDSIGFYRPIMEGLFLKKGNETGYFKLWMDWTGLQTNT
                     DRETFYLGASAYKQIRGPFFADFQSYMFHFAGMNPYNPDITVCDNLLAQLSVGVKYSN
                     IWVLNNLMASAGVLSGFERDRYDMNNYGTPTGAIFRINADYKGIGTENNLYFGQPHMV
                     LYRRYKNELYWGNPFYRDNYYIESKIFWNLIDTRTVKGQLAAKLHVTEGKLLFEQLFT
                     LRASIGSDQPKPRKTTLWDYFIKKKALPRNNFNL"
BASE COUNT         4247 a         2710 c         2904 g         4353 t
ORIGIN      
        1 cttttttatg taatatccgg ttacacttcg gaggtgaacg tcgatgcagg caatccggcg
       61 acattcataa agttggtgtg tatgtaaggc agacatacgt agcgcgtgag acattactta
      121 tttcctgcct gaaaataata ttatgaatac acttggtgtg tttcggactt tttacattca
      181 gaaataaata catcataatg atttatggtt aatcaaaacg ttttctgata atatctcttt
      241 aataactgat tgtgaaattt tcaagattct cagaaaatat ttgttgaagg cattagaaaa
      301 tcggttttta aattcttatt tttgcactta ttagataaac aataagatta tggataatat
      361 tgaaaaaagg aaaatatatt acacgttgtt ttttccggtt ttaattagtt tgtttatcgt
      421 tcttgttttt ctgtttgaga gaggaatggg ttgggatttt tataaagcag gcatatttcc
      481 tcatgatctg aaaacgttgc ctaacatttt tacaatgcct tttattcatg cagatttatc
      541 gcatctgttg aataatatta ttccattttt tgttttaagt agttgtttgt actattttta
      601 tagtcaaatt gcaaataaaa tattactttt gtcattaatc ctgaccggat ttattctttg
      661 ggttatcgga cgtgacaact ggcatattgg tgccagtggt ttggtttatt cattgtcatt
      721 ttttttgttc ttcagtggct tattccgaaa acacatcccg ttaattgcaa tctcatttat
      781 cgtagtgttt ctttatggca ataacgtctg gcatttgttc ccctggggga aaatggatcc
      841 cgtgtcgtgg gaaggacatc ttgctggagg tattacggga ttatttctcg ctttcatata
      901 caggaaacag ggaccgcaaa aacccgaaaa gatatgggac gatgaagatg ttgatgatat
      961 ggaagatatg gaagatgctg aaattcagga tgattataag gatgaaaagg atgatgcttt
     1021 atcagaaaat taattttagt cgaaatattt atggaaacaa aaaaaacgaa tgagatcaat
     1081 ttaatggatg tcattatcga aactggtaaa tggttaaaaa aaacagttgt tcaattattg
     1141 gagttttccg gtaaaatact gatactgctg tataaacaca aaatcattac tgcttgtata
     1201 ttaattcttt caatagctgt cgggcagtat aagtccagac cttcagcacg aaaatataat
     1261 atggagggaa tgtgtgtatt atatggtccg gaagcaaaaa ccatcagtga aatcggcaag
     1321 cagcttgcgt attcttcacc acgctttcag gctacttcac tcgcccgaaa attatcattg
     1381 ccggatagta ttgcctcaaa agtgactgga gtggaatttt ttcaggtgat agattatcag
     1441 cttaatcata ctccggattt gatcgatttt aaaaacagcc attctatgga agatacactt
     1501 aatctgccaa tgattgacta tatatacatg cggattaaaa taataggaac cagtaatgcg
     1561 aatgctattg gtgatgccat gctgaagtat attaatacca atcctaccgt acaaaatcag
     1621 tatgaaattc ttaaaggaca actgaatgaa aaaatccggc tttgtgatgc tgaaatgttg
     1681 cgactggata gtttggcaaa aattacttat ttcgaggaca aaaaacctga aatcaaattt
     1741 gaaagcaata aactgttgat aggtaataat actattcagc tattttataa tgacatgcta
     1801 actctgcaga catataaagc taatgctcag aatagactcc tcgatgctaa gcaaccggtt
     1861 gtggtacctt caggttttgt ggtaaatcca aaagcaatca acggacgagc aaaatacggt
     1921 atttatagtt tgatcatagg gctattgttg agcatattcg tcagtttctt tttagaaaac
     1981 cgaaaaaagt gggctaaata cctacaaaat aattaattgg tgtatatcac aacaaaaaaa
     2041 ggcatctgaa agatgccttt ttttgttgat cttaattgcc gaatacaatt ctaccgtcaa
     2101 cacaatcgac cagaaccggt ttctcgctgt tgacttttcc gcttataagt tctttggaca
     2161 gatcgtttaa tacatacttt tgtatggctc tttttaccgg acgggcacca aattgaggat
     2221 cgtatccttc ggccgaaata aattccattg ctctgtcggt tattttcaga tttataccat
     2281 tttcagccag catatttttc accgcgtaaa gctgcatttt tacaatttcc ataatctgac
     2341 tttcgtcaag tggtgcaaac atgatgattt cgtcaatacg gttcaggaat tcgggcctta
     2401 cagatttttt cagaagttca aaaacttcat tttttgtttt ttcaatcact tcggttcggt
     2461 tccggatgtt gatatgttca aagttttcgc ggataagcga tgaacctaca ttggaagtca
     2521 tgatgataat cgtgtttttg aaattgacag tacgtccttt attatcggtc agccgaccat
     2581 cgtccagcac ctgcaacaag gtgttgaata cgtccggatg ggctttttcg atttcgtcga
     2641 acaaaacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct gcagcatttt
     2761 ggttaccggt atgccggtcc atcggcttac cacatctgct atatcctgac tgtcaacctc
     2821 ttcttttatc atggcgttgt ttccctgtat ttcagccagc ctcgattgat tttccgaaat
     2881 cagattttct tctgatttaa tctttccgta acggatttca gctacccggc cataatcccc
     2941 ttctcgttca gcgcgatcgg cttcgtattt caggttttca atttcaattt ttgactgttg
     3001 aattttttca attatatctt tttcagacga ccatctggca cgatattttt tgctatcttc
     3061 tttcagattt gcaatttctt cctccagttg ttccagttta cggacatcat tttcgcgttt
     3121 gatagcttcg cgttctatct caagttgttt gatgttacgt tctatcttgt ccagttcctc
     3181 cggtacactg ttcacttcca ttctgagctt agcggctgcc tcgtccatca ggtcaatggc
     3241 cttatcgggc aggaaacggt cactgatgta gcgattcgag agttcaaccg cagcaatgat
     3301 agcatcatcc ttaatcctta ctttgtggtg attttcatat ttttccttca acccccggag
     3361 tatggatatg ctcgatgcaa tgttaggctc gtcgatcatg acaatctgaa agcgtcgttc
     3421 cagcgctttg tctttttcaa aatatttctg atattcgtcc agcgtagttg caccaacagc
     3481 acgaagttct cccctcgcca gtgctggttt cagaatgttg gcagcatcca tggcaccttc
     3541 gctttttccg gcaccgataa gggtgtgaat ttcgtcaatg aaaagtataa tctctccatc
     3601 cgaatgaata acctcattca ccaccgattt cagtctttct tcaaattcgc ctttatattt
     3661 tgctccggca ataagtgcac ccatatccag cgaatatacc tgtttcgttt tcagattttc
     3721 gggtacatcg cctcttacaa tacgctgtgc aagaccttcg gcaatggcgg tttttccggt
     3781 tccgggttcg ccgatcagga tgggattatt tttggtacga cggctcagta cctgcaaaac
     3841 acgccggatt tcctcatcac gtccaatcac cgggtccagt tttccggaac gtgcacgttc
     3901 attgagattg atagcatacc tgtcgagtga ctgataggtt tcctcggccg atgctgagtt
     3961 tgcaccggca cctttgcgga gttccgaaat ggctgcttca agcgcttttt cgtttacccc
     4021 ggcatccttc agtatttgag aaacgctgtt tttgacttta agcaaagcca gtaagatatt
     4081 ttccagcgac acaaactgat cgcctgccga ggcagaaatc tctgaagctt tgcggagaac
     4141 atcgtttgtc tcacggctca aataaggttc tcctcctgta actttcggaa aactttcgat
     4201 ggttcggtcg ataacttgct gaattaccga cggatatata cccattttcc caaacagaaa
     4261 ctgaatgaca tcttctcctt tgttcaggag agctttcatc aggtgtgtgg tttcaatact
     4321 ttgctggttg taactttgag ccagatcaat ggcattttgt accacttcct gagattttgt
     4381 tgtaaaatta gaaaaattca tatgctttgt ttttgatttt ttattgtttt gtgcttttac
     4441 cgttttatgt ttacaaaatg tttaccaacc tgaaaatcag acatattggc atgaaaaaat
     4501 gttgttttaa tatgggtaaa tgacaaaatg gcaatttatg ctgtcaaaat ggcattttta
     4561 ttttttttag tttactttga agcatgaaca agcctgaaaa tgacaggaga ttgaaaaaaa
     4621 tgattatttt tgcaagttaa atcttgtaaa ttaggaagat aatgcagaaa attgatcaca
     4681 acatacctga acctactctt agaaggttac catggtattt agcgtatgcc aagttggtac
     4741 aacattcggg tgaaccgttc ctgtcatcta cccagatagc caccaatatt gctgttgacg
     4801 cctccaaagt tgcaaaagat ttatcctacg ttcagatttc gggccggaca agggtaggat
     4861 atgaagtgaa tgagctggta aaagtccttg agtctttcct gggatttacc aatacacata
     4921 aagcattttt gttcggtgtg ggaagtcttg gaggcgccct gttgcacgac gacgggatga
     4981 aacaattcgg gctggaaata gttgcaggtt tcgatgtcaa atatgaactt gccggaacca
     5041 gcatcaaccg ggtccctatt catcatattg atcatttcag ggaattgcag aaacaaacgc
     5101 ttgttaatat cggagtgctt acagttccgg tggataaggc gcaggaaacg gcagagatga
     5161 tgattgatgg cggaattcag gctatctgga attttactcc tttcagaata cgtgtgccgg
     5221 ataatattat tgtgcagaat acatccattt acgcacatct ggctgtaatg tttaaccggt
     5281 tgaacgaact aaactcaaaa attcaggaat aatgaagata tttgctattg gtcagaatta
     5341 tgccgaacat aacagggaac ttaacagtcc cgatcccaaa gagcctgtaa ttttcatgaa
     5401 gcccgatacg gctgcactga aaaataataa acccttttac attcccgatt tcagttcgga
     5461 ggtccattac gaaacggaac taatcataaa aataagccgt cttggcaaaa atatagcgcc
     5521 caaatttgcg tatcgctact acgaagaaat tggtttggga gtggatttta ctgcccgtga
     5581 tatacagaga aaactgaaaa gtgaaggaaa accctgggaa atatgtaaag catttgacaa
     5641 ttcagctgta atcggcagtt ttttacccaa agaggaattt gcagatattc agtgtatcga
     5701 ttttcatctc aatattaacg gcaatcctgt acagcacggg aatacagccg atatgatatt
     5761 tccggttgat gaaattatcg cctacatcag caggtatttt acgctgaaaa taggtgatat
     5821 cctattcacg ggcaccccgg ttggtgttgg gaaagtgaat atcggcgacc atctcgaagg
     5881 ttttatacag gataagaaaa tgtttgattt taaggtgaaa taaacatttt atatcaccca
     5941 tttacaaaca ttctgaaaaa ataattgttg aatatatccg ttattaagag gagatacata
     6001 aaatacgagt tgaacgcatg aaatacaatt ttttcgccct atttctgaat attattccat
     6061 tccttacgtt gtctgcgcaa aacacgacaa tagagaagca tgtgataaac tggaccggaa
     6121 ttgaaacctg gcaagccgag aaatccagca aaaatgttct ttcgtttact ggtgcacaat
     6181 atccgttgga aaatgatttg ccgtattttg tagaaacgct agtttgggat aagaactaca
     6241 gctgcaaggt tcagattgaa aatcaggtat atgctgacct gacaattccc gaaattaatg
     6301 tcctcgaaaa caatgatatt ccgcaggaag ttgaagtgca aacttttatt tcacaggaaa
     6361 aaggcaattc ttaccccagt gtacgtttgc tgccgttgat tatgtctgat ggaaaggtga
     6421 agaaactgat cagctttgat ctgaaaatcg aaaaatcgtc aaaaccactc aaagctccgt
     6481 cttctgttca ttcgtatgtc aacacatctg ttcttgcgtc tggtaaattt gtaaaaatta
     6541 aaataaaaga tagtggcatt tacaaactaa cctatgaagc cctgagttcg atgggtgtaa
     6601 atcccgcgaa cgtaagggtt tttggctacg gaggtgcttt gcaggaacaa aatttcctga
     6661 tgccaaaacc ggacgacctc cccgaagttg ctatctggat ggaaaaaggc tctgacggag
     6721 tattcaatgc cggtgattac atcctttttt atggacaggg aattgttaaa tggaaatacg
     6781 attcatcaaa agaaatgttt gttcacacac tgaacaacta ttcaaccgat gcttattatt
     6841 tcgttacatc ggatgcaggc accggaaaaa aaattaccga acaaaccatc gaagttccgg
     6901 agaatacaat acaggagaca gtcactgaat tcgaagatta tcaggtgtat gaactcgaaa
     6961 agcaaaatct ggcacaaacc gggaaagtgt tttacggaga aacattttcg gatgtacttt
     7021 cctataattt cccgttttcc tttccaaatg taatcaaaac tgcgactaca aaagcacgat
     7081 tagacgtggc tgccatttca agtgagaata cctcctttgt tctgaaactg aatgatacac
     7141 aacaacagac agttactgta ttgaaaaaaa gtattgacaa ctacgaagtg ggcaaagatg
     7201 caaatgttgt ttttacttat actccctcag ccgataacct gattttcaat ctgtcctata
     7261 caaaagccac tcctgtttca agagggtatc tcaattatct ggaagtgaac gcacgtcgta
     7321 gtctgactat ggcaggagcg gtcatgtttt tcagaaatac cgattatctc aatttcggaa
     7381 cgtataatag gtatcagttg tccggtgcgg ggccaaatgt tcagatatgg gacgtaaccg
     7441 atccattgaa tatcaaaaaa atagtgacca cacggacaaa taacgttctg gagtttgttg
     7501 atacaaacag catgctgaaa gagtatgttg ccatcgatgc aacttcggct gcagcattcc
     7561 aacaaccttc cgtagtgggt gaggtgccaa atcagaattt acacggaatt cagaacgccg
     7621 agatggtaat tctgactcat cctacatttg tcagtcaggc tgaaaaactg gctcaggcac
     7681 atcgcgataa agataacatg acaatagcag tggtaacaac cgaacaggtt tacaatgaat
     7741 tttcgtccgg aacacctgat gccacagctt accgttgggt gatgaaaatg ctttatgacc
     7801 gggctatggc atccggtaat gcttcattag cacccaaata tttattgttg tttggccggg
     7861 gttcgtttga caaccgagga ttaattctaa attcgggcaa taacttaatc cttacctatc
     7921 aggctgatat gtcgctggat gtggttaaat catacgtaac cgatgactat tttggttttc
     7981 tggacgataa tgaaggctta cagatttcat cccacctgct ggatgtgggt attggtcggt
     8041 ttccggtggt gaccactcag caagcagaag atgtggtgaa caagacaatc cactatatgg
     8101 aaaataagga aaaaggcaac tggaaaaatc agatttgttt tctggctgac gatggcgatg
     8161 gtgctctgca tatgcgacag gctgacagta tcgtttcact ggtttcaaga aacaaccccg
     8221 gttttcagta caataaaatt tacctcgatt catatcttca ggaggttgca gccagtgggg
     8281 agtcttatcc cctggcaaaa acgaaatttc ataatatgct gagttccgga gtgtttctgc
     8341 tcgattatac cggacatgcc agccagctgg gatggaccaa cgaacagatt ttaaccactg
     8401 cggatattaa agaaatgtat aataccaggt tgcctttctg gattggtgca acctgcgatt
     8461 tcattctatt cgatgttaag gaaatttcag caggtgaaca tgtgctgtta aatccggctg
     8521 gtggtggaat tggacttttt tctgctgcaa gaacggtata tgcttcacaa aatgagaaac
     8581 ttaaccgcta cctgactgtt aatcttttca ataaacagaa tggcaagtac aatacacttg
     8641 gcgatgcagt aatgaaatca aaaaatcaag ttgggggcga aattaataaa ttatcatata
     8701 ctttgctcgc cgacccggca ttgcggctca actatcctac aaactacaaa gtaattaccg
     8761 caaaaatcaa caatcagccc gtgcagccta cagatacact tcgtgcacta tcggtaaact
     8821 ccgttgaggg atacatagct gatgataatg gaaataaaat ttccggattt aacggtaatc
     8881 tggaagttac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tctgatattc
     9001 atggtgttaa tacagtggga agcggtattg gacacgacct gagacttgtt attgatgata
     9061 atccaaatac ttcgtacata ctgaatgatt attatgaagc cgaaacgaat agttttaatt
     9121 cgggttcggt aaggtataaa ttaccagtca tgtctaaagg aaaacatacc ctgacatttc
     9181 atgcctggga tttgctgaat aactcatcgc agaaaaccct ggaatttgaa gtggtccccg
     9241 gacttactcc agtcatcttc aatgtgtata attatcccaa tcccgtaaaa agtcagacac
     9301 agtttgtggt cgaacacgac agacccgaaa caattctgga aacgacagtt gaactttttg
     9361 atatgatggg acgaaaaata tggtcgctca agcaaaacag tgtcgaaaat ctaaaatgga
     9421 atcgtgagga tacatcgctc agtcatgtga aacccggaat ttatatttac aaaatatcta
     9481 tcaaaacagc taacagcgaa ttgacatcaa aagcaaataa aattatcgta accggacaat
     9541 aaaaaagaaa atttacgtta atataattct acatatccac tttgtatcga attttaagaa
     9601 gtgacagaaa aataagcatt taacataccg ttaaactgtg agtaaaataa attatctttg
     9661 caaaaaatat aatacattac aaaacaaaat attacgaaac agatgaaaaa gaccaattta
     9721 atcttattct cgttgttatt aatcactctt tcttcctttg cacaaacatc agttaacgat
     9781 cagttaaacc ctctgattac cggagttcca tctttgacaa ttactccaga tgcccgtgcg
     9841 ggtggtatgg gtgatgtcgg agctgccacc actcccgata tgaattcgca atactggaat
     9901 ccagcaaaat actcatttat gaccagtccg gccggattat ctttttcctt cactccgtgg
     9961 ctccggagtt taggtatcaa tgatatcaac ctgagttatc tcaccggata ctggaaattc
    10021 gatgatatgc agtctgtcag cggttcgttg cgttattttt cgctgggaag cgttcgtctc
    10081 acagacgaac agggcaatga ccttaatatg gatgcacaac ccaatgaact tgcactcgat
    10141 gtgtcatatt caagacttct ttctgacaat tggtcagctg cagttggctt tcgttacatc
    10201 cgttccgacc tgaacaatgg tgcaaatggt gcaggtggaa tcgaaatgtt tcccggaaat
    10261 gccttcgctg cagatgtagc cacctactat aaaaccccca ttccgatggc taccggcgat
    10321 gcaaatcttg ctttcggttt aaatatatcg aatatcggtt ccaaaatatc gtatgatctg
    10381 aatgaaacca gcatttttat tcccactaat ttaagactcg gtggctcgtt cgaatatccg
    10441 ttcgatgact ataataaaat ttcattcagt gctgacgtca ataaactgtt ggtgcccact
    10501 ccggtaaagc cgataggtga cgaaacttat gaatcgcaaa gctatattga tcgaaaaaat
    10561 gtatacgatc agactggccc tattgccgga attttcaaat ccttcaacga tgcaccaggt
    10621 ggaatgtcgg aagaactgaa ggaaatcatg tggtctgccg gtgctgagta tgcttacaat
    10681 aatcagttct tcgttcgtgg aggatatttc aatgaaaatg ccatgaaagg taaccgtaag
    10741 tatttcactg ccggggccgg gtttaagctc aatgtgtttc agctcgatgt agcctacctc
    10801 atttcggcag cacaatccaa tccgctagat cagacattgc gattttcgct cgcattcgac
    10861 ctgaacgggc tcactgatct tatgcgataa aatttgttga agataaatac agaaaacggt
    10921 tgtgtcagtt ggttgataaa caaccgtttt tttgtttaag acagattagt aaaattcaag
    10981 caaccattca aaaattaaaa tattaacata tgaagatcag agttggtttt ggatatgatg
    11041 tacatgcatt tgcacctgaa cgtgagttat ggcttggtgg tgtgaaaatt gaacataatc
    11101 tcggtctgct gggacattcc gatgccgatg tactaattca tgcgctctgt gacgcgttgc
    11161 tcggtgctgc aaatatgcgt gatatcggtt atcacttccc ggatacttcc attcattata
    11221 agaatattga cagtaagctg ctcctgatgg aaaccatgtt gcttattcgg gggaaaggtt
    11281 acgaatttgt caatgccgat tgtactgttt gtgccgaaca acccaaactc aatccgcacg
    11341 taccttccat gcagcagacg ctttcgggag tgatgggtgt agatgaagac gatatctcga
    11401 ttaaggcaac aacctccgaa aagatgggat ttgtaggacg acaggaagga attgctgctt
    11461 atgctaccgt attgattcag aaacagtaaa acagggttat catttttgtt aattggccta
    11521 taaattttat ctatcgttat tactgattat ttaatttaat tttgtgtcat aaaaaatcat
    11581 ttaaaattaa aatattatgt cagtattagt aggaaaaaaa gcaccggcag tagccggtaa
    11641 agccgtagta aatggcaacg agatagtaga taacttttca ctggaaaatt acagaggaaa
    11701 atacgtcgta tttttcttct atcctgccga ttttaccttt gtatgtccga cagagttaat
    11761 cgcttttcag gaaagaattg cagaatttga atcacgcaat acagcagtta ttggcgcttc
    11821 aaccgattcg gaattttcac attggaaatg gctccagaca cctcaaaatc agggcggaat
    11881 tcagggtgta aaatacccga tttatgctga ccagaacatg actgtatcta aagcatacga
    11941 tgtactggcc ggatatgaag aatataccga agaaggagaa ctggtttttg tcggagcacc
    12001 tcaggcttac agaggtttat ttttgatcga caaggaaggc gtggtacgtc accaggttgt
    12061 caatgatatg ccactcgggc gaagcgttga cgaagtaatc agagtgcttg atgctttgca
    12121 atttacagaa gaatatggcg aagtatgtcc tgccaactgg aaaaaagggg ataaagctct
    12181 caagcccaca caggaaggaa tttctgatta tctgtcacac taattgtaat aactaataaa
    12241 aaaggctgtt tttggaataa tcagaaacag ccttttttat tgcttaaatt tgcataaacc
    12301 ggattatttt acaaaatttg tagtttaaaa caaaaggtaa ggacatctcc ccctttaaat
    12361 attttagaaa tggatatgac atggctttat gccttagtga gtacgcttgt tgttagcttg
    12421 atatcattga ttggtgtttt tttgttatca ttcaaggcgc agcagctgca tcgggtatta
    12481 attttactgg tttgtttctc gattggtgcg atgcttggaa atactttttt tgaacttcta
    12541 cccgaatcat tcctgcataa ttccgacgcg agtctcacag gttggctggc aatcggcgga
    12601 tttctggtgt tttttgtcct cgaacaattt ctgcacatgc atcagcatag ccattccaaa
    12661 gggaaaggat atattaaatc atatggttat ttaagtctct atgccgactc tatacacaac
    12721 ttcactgatg ggattctgat aggagcggcc tggatgttta cacccgaaat aggacttgca
    12781 acaacatttg ccatattgtt gcatgaaatt ccacaggaaa taagtgattt cggcatactg
    12841 cttcaggctg gtttctccaa gcgtaaagct ttgttgaata atttttatgc tgcctgcaca
    12901 gccattctgg gtactatcat caccctcgct gtaggagaag aagttgaaca tctctccgtt
    12961 tatattttac cggtcgctgc cggaggattt atttatcttg cctgcagcag tctgttaccc
    13021 gagatactga aggaaaccac aaagaaaaat tatttaattc atttattatt tatattgctg
    13081 ggcttatatt taatgtattt ttttagtatt cacggtggtc atcaacattg attttatgaa
    13141 aaaatatctg atttctatta ttcttttcat tacaggttta tcgatttcaa catcggcgca
    13201 ggaactccag tggaatttga atctgaatta cttcttcgat aatactgaat ttgcaaggtc
    13261 tgcactatcg aatgaccaaa ctatgacagg ggtgcatttt tcacccgaat taggaatgaa
    13321 gtgggatgaa aataactcaa tatatggcgg agtggatttg ttgaaaaaat cagcttcaaa
    13381 aacgttcgcc gataacgtga agtttatcgc ctactatcag ttcagaaatg aaaacacgct
    13441 attcaaagcc ggtgcatttc aacggggaga tttgctttcc gattattcaa ccgcgttctt
    13501 tcaggactcc atcggttttt atcgaccgat aatggaaggt ttgtttctta aaaaaggaaa
    13561 tgaaaccgga tattttaaat tgtggatgga ctggacgggt ttgcagacca atacagaccg
    13621 cgaaaccttt tatctgggag catcggctta taagcagata aggggaccgt tttttgctga
    13681 ttttcagtcg tacatgtttc atttcgcagg catgaatcct tataatccgg atattactgt
    13741 atgtgataat ttgcttgctc agttatcggt gggagtgaag tattccaaca tctgggtatt
    13801 gaataattta atggcttccg ccggagtatt atctggattt gaacgtgaca ggtatgatat
    13861 gaataattat ggaactccta ccggtgccat atttcggata aatgcagatt acaaagggat
    13921 cgggacagag aataatctgt atttcggtca gcctcacatg gtgttgtacc gaagatacaa
    13981 gaatgaacta tactggggca atccctttta ccgtgataat tactatattg aaagcaagat
    14041 tttctggaac ctgattgata caagaacagt gaaaggtcag cttgcagcaa aactacatgt
    14101 tacggaagga aaactattat ttgaacaact tttcaccctt cgggcgtcca ttggcagcga
    14161 tcagccaaaa ccgagaaaaa caaccctttg ggattatttc ataaagaaga aagctttgcc
    14221 cagaaataat tttaatctat agaattaata atctttcggg aagcaaggag agaattatta
    14281 ttttgtaagc ataaaaccaa taacccctcg acagtaacga gggttgttgg ttttttagta
    14341 tttaaaacta gctgtcagta ggatggacat gggcctgatg ccatatttgt acgaatagtt
    14401 atccaaaccg ttga
//
LOCUS       DAOPYB010000063        14336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4603_length_14336_cov_9.052307, whole genome shotgun sequence.
ACCESSION   DAOPYB010000063 DAOPYB010000000
VERSION     DAOPYB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14336
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4603_length_14336_cov_9.052307"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            550..930
                     /locus_tag="PKH58_06570"
     CDS             550..930
                     /locus_tag="PKH58_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNX88727.1"
                     /translation="MDYTEENQLSNIIIGCAIEVHKQLGPGLLESAYQECLQYELIKA
                     GLEVIKEKPMPIIYKDVKLDHGYRIDLLVNNKVVIEIKTVEQFTEVHTAQVLTYLKLG
                     NYKLGILLNFYVTTLKDGIKRVIN"
     gene            1045..2289
                     /gene="rocD"
                     /locus_tag="PKH58_06575"
     CDS             1045..2289
                     /gene="rocD"
                     /locus_tag="PKH58_06575"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNX88728.1"
                     /translation="MKNNKKYSAEELMELENRYGAHNYHPLPVVLEKGEGVYLWDVNR
                     KKYYDFLSAYSAVNQGHCHPKIVETLTEQAQKLTLTSRAFYNDKLGVYEKFVTGYFGY
                     EKVLPMNSGAEAVETALKLCRKWAYQKKKIAENDAQIVVCAENFHGRTTTIVSFSVDP
                     DAYTGYGPFTPGFIIIPYNDVEALRNVLNENRNVAGFLVEPIQGEAGAKVPDDGYLRQ
                     TYELCKENNVLFIADEVQTGIARTGKLLACDYEEVKPDILILGKALSGGVYPVSAVLS
                     NREIMDIILPGQHGSTFGGNPLAAAVAVAALEVVRDERLAEKAYELGIIFRREMTEFC
                     KTSKVAEFVRGKGLMNALIINNADQKNLAWDICLEMAENGLLAKPTHTNIIRFAPPLV
                     ITETQLRECIEIIKYSVSKFEK"
     gene            complement(2563..3123)
                     /locus_tag="PKH58_06580"
     CDS             complement(2563..3123)
                     /locus_tag="PKH58_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88729.1"
                     /translation="MKKAAFIILLIGMLSAHNLKAQNKVSFFEEHIDFSIDKDYFTIN
                     GIYSFCNHSGVKVNQTILFPFAVEVTEIDSIRIVNLKTSSGIGFNPSGKLISFTIPIL
                     PNDTVDINLFYRQKTSVSNKYIITSTKAWGKPLEKADYSLTADKDISIKTFSYTPDSF
                     NKKRKIYFWHKENFMPETDFIVTLND"
     gene            complement(3120..4208)
                     /locus_tag="PKH58_06585"
     CDS             complement(3120..4208)
                     /locus_tag="PKH58_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX88730.1"
                     /translation="MKKLLLAITIIVNIQLLNANPLPPPAPVIVLSEFRFETDGKWVL
                     ELYNRNDYGSSMTDSVFVITSSGRAKLKSFIPQGYEYRRFVIVRNEDMLSDLSVNTNE
                     DSIKVEYHYIDYNEWGNDARKTRYTNTIKFGKTDKGYMPAPKTGESVAEYNGEYGLDK
                     SPTINAQNDTVGMVMGTMRGSLYDEENNLLKSYPYEFYGNGFAAFRTDENGTFSTRVL
                     LNNYNITSICYDDDGDYNQNYKLAEINPVNVKFSPDTVINADIHIIKRTAMGLNEINS
                     GNISVLNVFPNPLKDKSFNYETTIPVKSTNSYLEIADINGKKIARYPVEEQKGYIKLP
                     LSITKGTYVVKLMVNSKNYGSTKVLVEE"
     gene            4624..6231
                     /locus_tag="PKH58_06590"
     CDS             4624..6231
                     /locus_tag="PKH58_06590"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNX88731.1"
                     /translation="MKETKYIFVTGGVSSSLGKGIIAASLGKLLQARGFRVTNQKFDP
                     YINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFLNIETHRANNITTGRIYQNVIN
                     KERRGDFLGKTVQVIPHITDEIKRNIKILGSKGEYDVVITEIGGTVGDIESLPFIESV
                     RQLKWELGKNCLIVHLTYVPYLAAAKELKTKPTQHSVKMLQEQGIQPDILVLRAEHDI
                     PMDLRKKVALFCNVEPTAVMQSIDASTIYRVPLNMQKEGLDKVALTKMGYDLTKLPEP
                     DLDKWIEFVEKMEGATEVVNVALVGKYVQLQDAYKSISEALMHASAYSNRKLKLDMIL
                     SDKITPENVEQKLGKKDAILIAPGFGQRGMEGKFTALRYARENNVPCLGICMGMQTMC
                     IEFARNVLGYDDANSTEIEPHTTHNVVDIMDEQKNILNLGGSMRLGAYKCKLKKGSRA
                     YQIYGKENISERHRHRFEFNNKYKEEFENAGMICSGLNQESDLVEIVELKNHKWYVGV
                     QFHPEYSSTVVNPHPLFCSFIKAAIEK"
     gene            6349..8265
                     /gene="yidC"
                     /locus_tag="PKH58_06595"
     CDS             6349..8265
                     /gene="yidC"
                     /locus_tag="PKH58_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNX88732.1"
                     /translation="MDKNTIIGFILIAAIIFGFAALNKPSEAEIAKRKRYNDSIAMVQ
                     QQQAAMQQKIDSTKQYLANADSTVLNDSAAIAQKSDAFGAFSPSAFGEQKFITVENKL
                     IKLKFSNKGGRIYSAELKNYRTHDSLPLILFDKEESTFGFTLTTNNNRVVNTSDLYFD
                     QIGDIVKDKNGNQSVVLRLKTSQPASYIDFIYTIPANDYMVKYDVKASNMNAIIPPSM
                     NLLEMNWTSKIRQQEKGRQFEERYSALHFKYLADDMEKLSESKNDQEEVNNKVKWIAF
                     KDQFFSSILIADESFVSTMFNSEMMPEKSGFLKSFSSKMSVGFDPTGNQPTGFRFYFG
                     PNQYKILSAYDKGIPGDQKLELRDLIPLGWTIFGWVNRYMIIPMFNFFSTFIGSFGLI
                     ILLMTIVIKILIFPMTYKSYLSSAKMRVLKPEIDEINARIPPEKALERQKATMDLYNK
                     VGVSPMSGCLPMLLQMPILFAMFSFFPSAIELRQQSFLWAKDLSAYDSILSWNAYIPL
                     ITPFFGNHISLFCLLMTISSIISTKLTTAQTPTASDQPGAGMMKWMMYLMPVMFMFMF
                     NNYASGLSYYYFLSTLITIGQTYLIRATVDDKKLLAQLHAKGNKAKPKKKSGFMERLE
                     KMQREQEKALKNRK"
     gene            8622..9632
                     /locus_tag="PKH58_06600"
     CDS             8622..9632
                     /locus_tag="PKH58_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88733.1"
                     /translation="MKRLNYIILLISLLFTFTGCTKKSRFEINTSKEKVDVTIHRFDK
                     DLINLDISKLNQSVDALYKTYSSFLPLYIYNVLDTLPEDTLAVAGMMKQFVQDTSFIS
                     VNKKVMETFSDISDIEKDISGGFTYIHHYFPKLPLPEVYFFVSGFNRSVLMTDSILGV
                     GTDFYLGADYEPYKSFTYDYLLQNMRRESLAIDVVSATLFRYFSFDGTNNALIDNMLH
                     RGKVIYLLSVFMPGKKMNDIIGYTTEQWKWAEQYEEEIWKTVVGKKHLFSTDMQLISK
                     YMNDAPFTSLVSQDSPGRLGTWIGWRIIKSYMERNENVTLEQLMKENNYRKILEQSHY
                     KP"
     gene            9715..10407
                     /gene="radC"
                     /locus_tag="PKH58_06605"
     CDS             9715..10407
                     /gene="radC"
                     /locus_tag="PKH58_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNX88734.1"
                     /translation="MEEHYLSIRNWAEDDRPREKLLKKGVSALSDAELLAILIGSGSK
                     NESAVELCRKILKSSHDNLNELARLSINELCRFKGIGEAKAVTICAALELGKRRKTNE
                     VLERMRISQSSDLYELFEPILLDLKHEEFWVAYLNGANKVIDVKKLTQGGSRQTVVDI
                     QMILKMSLEKSAQAIAVAHNHPSGQNYPSREDETITQRVKKGCEAIDVRFLDHIIIAG
                     GKYFSFADEGKI"
     gene            10430..11347
                     /locus_tag="PKH58_06610"
     CDS             10430..11347
                     /locus_tag="PKH58_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX88735.1"
                     /translation="MNLLEVNNVVKQYSGQLALDGVSLTVPKGTVYGLLGPNGAGKTT
                     LIRIINCITAPDSGEILLNGKKLSPKDVRNIGYLPEERGLYKKMKVGEQAMYLAQLKG
                     MTKHDATKALKYWFEKFEIQSWWNKKVEELSKGMAQKVQFITTILHNPDLLIFDEPFS
                     GFDPVNADMLKKEILALRDKGATIIFSTHNMESVEELCENISLVNKAHIVLQGNVAEI
                     RASYSTRTLMVRSAAPVSSDSCEIVSQNKVNGVFETSLRMIKEMSNNDFLTELMKQTE
                     IHSFEELLPTMNDIFISTVKNSENINNRL"
     gene            11344..12675
                     /locus_tag="PKH58_06615"
     CDS             11344..12675
                     /locus_tag="PKH58_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX88736.1"
                     /translation="MSKIGIIIRREYSSRVMKKSFLLLTFLTPLLFVAMIMIPLWLSS
                     IEDDTIKNIIVIDKTGIYHDALKSNETCTFLFSDAPLDNYKRKDNKESEMTAVLYITA
                     DLNKQPDAVKIYSEKQVNVELKSYISGLLEDYIQKQNLNAYNIPNLKELVEKSKTKLD
                     VKTIKWDAEGKEKEASAELALIIGMISAFLIYIFIMSYGSQVMSGVLQEKTSRIVEVI
                     ISSVKPFELMMGKIIGIALVGLTQFFLWIALTLIISAGVSAFMGGSFNPEALQTAQQI
                     NMPGMQTNVQQTETQAMIAEIYTSLNGFDFFKTLVLFIIYFLGGYLLYASLFAAIGSA
                     VDNETDTQQFSMPLTIPILFAIYAAIYSAQNPDGPLAFWCSLIPFTSPIVMMVRLPFD
                     VPGWEIVLSVFILILSFIGTTWMAGKIYRTGILMYGKKVSWKELWKWLTYK"
     gene            complement(12742..12900)
                     /gene="rpmH"
                     /locus_tag="PKH58_06620"
     CDS             complement(12742..12900)
                     /gene="rpmH"
                     /locus_tag="PKH58_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNX88737.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMASANGRRVLAARRAKGRAKLTV
                     ADEGRHKF"
     gene            complement(13047..13793)
                     /gene="fabG"
                     /locus_tag="PKH58_06625"
     CDS             complement(13047..13793)
                     /gene="fabG"
                     /locus_tag="PKH58_06625"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNX88738.1"
                     /translation="MKLLEGKVAIVTGAARGIGKSIALKFASEGAHVAFTDLVIDENG
                     LTTQKEIEAFGVKAKGYASNAASYDDTHKVVEEILKDFGRIDILVNNAGITKDGLMMR
                     MSEQQWDAVINVNLKSAFNFVHACTPIMMKQKSGSIINMSSVVGVSGNAGQTNYSASK
                     AGLIGLAKSIAKELGTRGIRANAIAPGFIETEMTHALNDEQRAKWAETIPLRRGGTPD
                     EVANVALFLASDLSSYVSGQVINVCGGMNT"
     gene            complement(13944..>14336)
                     /locus_tag="PKH58_06630"
     CDS             complement(13944..>14336)
                     /locus_tag="PKH58_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88739.1"
                     /translation="EVYTPTSYTTSLPNGTYPVKINTVQNQTVIDGFNNGSEDLGTWY
                     YTADAFPITGGSMTVIKGTGSNYILNYTFIDELGNTITGNYDGILGLVNKENTSQGVK
                     SRIQKIKNYKNATSQTDKYSLNIHRGKR"
BASE COUNT         4361 a         2719 c         2975 g         4281 t
ORIGIN      
        1 aggctaacaa tccgggtttt tagtttgaag tgattgtttt tcttctttgt gttcctttgc
       61 gtcttaattt tgtggaactt tgtgtaagag ctattacaca aaagtcacaa aggagtcaca
      121 aagattataa atcatattag gctaacaatc cgggttttta gtttgaagtg attgtttttc
      181 ttctttgtgt tcctttgcgt cttaattttg tggaacttag tgtaagagct attacacaaa
      241 aggcacaaag gagacacaaa gattaaaatc atattaggct atcaaaccgg tttttagttt
      301 gaagtgattg tttttcttct ttgtgttcct ttgcatctta attttgtgta actttgtgta
      361 agagctatta cacaaaagtc acaaaggaga cacaaagatt acatttcata taaggctaac
      421 aatccgggtt tttagtttga agtgattgtt tttcttcttt gtgttccttt gcgtcttaat
      481 tttgtggaac tttgtgtaag agctgttaaa acaaaacaca ctgaaaggtc acaaaataca
      541 caaataacca tggattatac agaagaaaat cagttatcaa atattattat tggttgtgcc
      601 atagaagttc acaaacaatt aggacctgga ttattagaat cagcttatca ggaatgttta
      661 caatatgagc tgataaaagc aggtttagaa gttattaagg aaaaaccaat gccaattata
      721 tataaagatg tgaaattaga tcacggatat agaattgatt tattggtaaa taataaagta
      781 gttatagaaa ttaaaacagt cgaacagttt acggaagtac ataccgctca ggtattaacc
      841 tatcttaagt tgggtaatta taaactggga atattactca acttctatgt aactactctg
      901 aaagatggaa taaaaagagt aattaattag taatagaaca tcagtaaaag cacatttaac
      961 cacgtttttt attaaaactg tataaatatc ctgattttac aattttattt cctatatttc
     1021 gggcaaatta ataacagaca aactatgaaa aataacaaga aatactcagc agaagaattg
     1081 atggaacttg aaaaccggta tggcgcacat aattatcatc cgctgccggt tgtgctcgaa
     1141 aaaggggagg gcgtgtacct gtgggatgtg aaccggaaga agtattacga tttcctttcg
     1201 gcttattcgg ctgttaacca gggacactgt caccccaaaa tagtggaaac actcacggaa
     1261 caggctcaaa agctgacact tacttcgcgg gcattctaca acgacaagct gggtgtgtac
     1321 gaaaagtttg taaccgggta tttcggctat gaaaaggtac tgccgatgaa cagcggtgcc
     1381 gaagctgtgg aaaccgcgct gaaactttgc cgtaaatggg cctatcagaa aaagaaaatt
     1441 gctgaaaatg atgctcagat agtggtttgt gccgaaaatt ttcacggccg aaccaccacc
     1501 attgtttctt tctcggtcga tcccgacgct tacaccggtt acggaccctt tacacccgga
     1561 tttatcatca tcccgtacaa cgatgtggag gcgctccgca acgtgctgaa tgaaaacagg
     1621 aatgtggccg gatttctcgt ggaacccatt cagggcgaag ccggtgcaaa ggtgcccgac
     1681 gacggatatc tgcggcaaac atacgaacta tgtaaggaga ataatgtgtt attcatagca
     1741 gatgaagtcc agacgggtat tgcccgaacc ggcaagctgc ttgcctgcga ctacgaagag
     1801 gttaaaccgg atatcctgat cctgggaaaa gccttatcgg gaggagttta tcctgtttcg
     1861 gcggtattat cgaatcgtga aatcatggat attattctac ccggacagca tggatctaca
     1921 tttggcggaa atccgctggc tgccgccgtg gctgtagccg ctctcgaagt ggttcgcgat
     1981 gaacggctgg ctgaaaaggc gtatgaactg ggaattattt tccgccggga gatgaccgaa
     2041 ttctgcaaaa cgtcgaaagt cgccgaattt gttcgaggta aaggattgat gaacgcgttg
     2101 ataatcaaca atgcagacca aaagaacctt gcctgggata tttgcctcga aatggccgaa
     2161 aatggactgc ttgccaaacc aactcatacc aatatcatac gatttgcacc gccgctggtt
     2221 atcacagaaa cacagctccg cgaatgtatc gaaattatta aatactcggt gtcgaaattc
     2281 gagaagtgat tattaatcac tgttgtccga taaaacttaa tattttgcca cttccgatgg
     2341 atcggaatgt tccatctgag gcaaaaaatc ttgtttttca catcttacta caaagattta
     2401 gccactatgt ggctgttttt gcaacagcgt tgcaaaatat ttgtagcgat ttggttacaa
     2461 caggaaagca agccccggtg gggcttaatg tttttatttg ttccgattac agtaaattac
     2521 agatgtttct gtgttttttt gtcggacaac aataattatt tctcagtcgt tgagcgttac
     2581 aataaaatct gtttccggca taaaattttc cttatgccag aaatagattt ttcttttttt
     2641 gttgaaagaa tcgggggtat aagaaaaagt tttaatggag atgtctttgt cggcagtcaa
     2701 tgagtaatca gctttttcca gcggttttcc ccatgccttt gtcgatgtaa tgatgtattt
     2761 gtttgaaacc gatgtttttt gtctgtaaaa gagatttatg tccaccgtgt cgtttggcag
     2821 aataggaata gtgaagctga tcagtttgcc tgacgggtta aatcctatgc cggaacttgt
     2881 tttaagattt actattctga tggagtcgat ttccgtaact tcaaccgcga aaggaaacaa
     2941 tattgtctga ttaaccttta cgccggaatg attgcagaaa gaataaattc cattgattgt
     3001 aaaataatcc ttatcaatgc tgaagtcaat gtgctcttcg aagaaagaca ccttattctg
     3061 agccttcagg ttgtgtgcgc tcaacatgcc aatcaacaga ataatgaatg cagccttttt
     3121 cattcttcga ccaatacctt tgttgagcca tagttcttac tgttcaccat tagttttacc
     3181 acataagttc cttttgtaat actcagtggc agtttaatat accctttttg ttcttccacc
     3241 ggataccggg caatcttttt gccgttgatg tccgcgattt ccaggtaact gttggtcgat
     3301 tttacgggaa tggtggtttc gtagttgaac gatttgtctt ttagtggatt agggaagacg
     3361 ttaagaacag aaatattacc ggaattaatt tcatttaatc ccattgctgt tcgcttgatt
     3421 atgtgtatgt ctgcattgat aacggtatca ggcgaaaatt tcacatttac cggatttatt
     3481 tcagccagct tgtaattctg attataatca ccatcatcgt catagcagat ggatgtgatg
     3541 ttgtagttgt ttaataaaac ccgcgttgaa aacgttccgt tttcgtctgt tctaaatgcc
     3601 gcgaacccgt tgccgtaaaa ctcgtaggga tatgatttca ggagattatt ttcttcatcg
     3661 tacaatgaac cccgcatggt tcccatgacc attccaactg tgtcgttttg tgcatttatt
     3721 gtaggagact tatccaaacc atactcaccg ttatattctg caaccgactc gccggtttta
     3781 ggagcaggca tatacccttt atccgttttc ccaaatttaa ttgtattggt gtatcttgtt
     3841 tttcgcgcgt cattacccca ttcgttatag tcaatataat gatattccac tttaatactg
     3901 tcttcgtttg tgttaaccga taaatcagag agcatatctt catttcttac tattacaaat
     3961 ctgcggtatt cgtaaccctg aggaataaag cttttcaatt tcgccctgcc ggaggatgta
     4021 atcacaaata cgctgtccgt catactgctg ccataatcat tccggttata caattcaaga
     4081 acccatttgc catccgtctc aaaacgaaac tccgaaagaa caataaccgg agccggcggg
     4141 ggcaatggat ttgcattcag taactgaatg ttcacaataa tggtaatagc aagtaaaagt
     4201 tttttcatga gatcagtata ttgcaggcat taacttttgc aaaagtaggg aaacttttat
     4261 taatatattc gtattattgt gtaaaagagt taggctgatc gtgattggcg gattaatcag
     4321 gttgagaatg cagctttatt atatgaaatt gatgcttttt tatggtatct gttttactta
     4381 ccgacctttc agggtaatga gtattgctgc aaatcatctt ttactcatat tggttaaatt
     4441 cacaaaacaa actaattcag ataaacttaa gtgttttttg ttgaagtaaa taaataacat
     4501 ccggaccatt ggttgtaaaa agtgtgaatt ttggattgtc tatttattta ttcgtttttt
     4561 tttccgaaat ttgcaacctt aaaattcgat tttcatacga atcattttaa gtaaaaaaac
     4621 aacgtgaaag aaacaaagta catatttgtt accggcggtg tatcatcgtc attaggcaaa
     4681 gggataattg cagcttcgct tggcaaactg cttcaggcgc gtggattcag ggtgactaac
     4741 cagaaatttg atccctacat taatattgat cccggaacgc tgaacccata cgaacatgga
     4801 gaatgttacg ttactgtcga tggacatgaa gccgatcttg atctcggaca ctacgaacga
     4861 tttctgaaca tcgaaacaca tcgtgccaac aatatcacca ccggacggat ttatcagaat
     4921 gttataaaca aggaacgtcg cggcgatttc cttggtaaaa ccgtacaggt tattccccat
     4981 atcacggatg aaataaaacg taacatcaag atacttggaa gcaagggaga atacgacgtt
     5041 gtgattaccg aaatcggcgg aacggttggc gatattgaat cgttgccatt tattgagagc
     5101 gttcgtcagt tgaagtggga gcttggcaag aactgtctga ttgttcatct gacctatgta
     5161 ccgtatctgg cagctgccaa agagctgaaa accaaaccga cgcaacattc ggtaaaaatg
     5221 ctgcaggaac agggaattca gcccgatatt ctggtgcttc gtgccgaaca cgatatcccg
     5281 atggatttac ggaaaaaggt ggctttgttc tgtaacgtgg aacctaccgc tgtcatgcaa
     5341 tcaattgacg cttcgaccat ttaccgtgtt ccgctgaata tgcagaagga aggtctcgat
     5401 aaagtcgcgc tcactaaaat gggttatgac ctgacaaaac ttcccgaacc cgatctggat
     5461 aagtggattg aatttgttga aaagatggaa ggtgccaccg aggtagtaaa tgtagcttta
     5521 gtggggaaat atgtgcagtt gcaggatgcc tacaaatcaa tttccgaagc gttgatgcac
     5581 gcgagcgcgt attccaaccg gaagttaaaa ctcgatatga tcctctccga caagattact
     5641 cccgagaatg tggagcaaaa actcgggaag aaggatgcca ttctgatcgc ccccggattt
     5701 ggtcagcgtg gtatggaagg caaattcacc gcgttgcggt atgcacgcga aaataatgtt
     5761 ccatgtctgg gcatctgtat gggtatgcag accatgtgta tcgaatttgc gcgcaacgta
     5821 ctcggatatg atgacgccaa cagcaccgaa atagagccgc acacaactca caatgtggtt
     5881 gatataatgg atgaacagaa aaatatcttg aatctgggtg gaagtatgcg tctgggagca
     5941 tataaatgca aactgaaaaa aggcagcaga gcttatcaga tttacggaaa agaaaacatc
     6001 agcgaacgac accgccatcg cttcgaattc aataataaat acaaggaaga attcgaaaat
     6061 gcaggtatga tttgcagtgg tcttaaccaa gaatccgatc tggtagaaat tgtagagcta
     6121 aaaaatcaca aatggtatgt tggagtacag tttcatcctg aatacagcag tactgttgtt
     6181 aaccctcatc ctttattctg cagttttatt aaggctgcga ttgaaaagta aaaatcataa
     6241 attaaataga ttataaatac cagtcagatt ttcagattat tcctctgcct gactttcagt
     6301 acagagatgg aaggaatatg aaatgattaa atctgtaaat aattaaatat ggataagaat
     6361 acgattatcg gttttatttt aattgcagca atcattttcg gctttgcagc gttgaataaa
     6421 ccctcggaag ccgaaattgc aaaacgcaaa aggtataatg attcaattgc aatggttcag
     6481 caacagcagg ctgccatgca acagaaaatt gattccacaa aacaatatct ggctaatgcc
     6541 gatagcacag tactcaatga ttcagctgct attgctcaaa agtctgatgc ttttggtgca
     6601 ttcagtccct ctgcttttgg tgaacaaaaa ttcattaccg ttgaaaataa actgataaaa
     6661 ctcaaattca gcaataaggg tggccggatt tattctgccg aactgaaaaa ctaccgtaca
     6721 catgactcgt tgccgctgat acttttcgat aaggaagaaa gtacatttgg ttttaccctg
     6781 actacaaata ataatcgggt ggtaaatact tccgatttgt attttgatca gatcggggat
     6841 attgtgaaag acaaaaatgg gaatcagtcc gtagtgctac gactgaaaac cagtcagcca
     6901 gcttcgtata tcgattttat ttacaccata ccagccaacg attatatggt gaaatacgat
     6961 gtaaaggcat caaacatgaa tgcgattata ccgccttcga tgaacctgct cgagatgaac
     7021 tggacttcca aaatacgtca acaggaaaaa gggcgtcagt ttgaagaaag atattcagct
     7081 ttacatttca agtatctggc tgatgatatg gaaaaactga gtgaatccaa aaacgatcag
     7141 gaagaagtga acaacaaggt gaaatggatt gcatttaagg accagttctt cagctccata
     7201 ctgattgccg atgaatcatt cgtttccact atgttcaaca gtgaaatgat gccggaaaaa
     7261 tccggatttc tcaaatcatt ttcctctaaa atgtctgttg gatttgaccc gacaggtaat
     7321 cagcccaccg gttttcgttt ctatttcgga cctaaccagt acaaaatcct gtcagcttac
     7381 gataaaggta ttcccggtga ccagaaactt gaactgcgcg atttaattcc acttggctgg
     7441 acaattttcg gatgggttaa ccgctacatg atcattccga tgttcaactt cttcagtacg
     7501 tttatcggaa gtttcggatt gattatcctg ttgatgacta ttgtaatcaa gattctcatc
     7561 ttcccgatga cttataaatc gtatttatca agcgctaaaa tgcgcgtgct gaaacccgaa
     7621 atagatgaaa taaacgcgcg gataccacca gaaaaagcat tggaacgtca gaaagcaacg
     7681 atggatctgt ataacaaagt cggtgtaagt ccgatgagcg gatgccttcc catgttgctt
     7741 cagatgccta ttctgtttgc catgttcagt ttcttcccgt cagccatcga acttcgtcag
     7801 cagagcttcc tctgggcgaa agacctttcg gcttacgatt ccatcctgag ctggaatgca
     7861 tacataccac ttattacacc attctttggt aaccatatca gtttgttctg cttgctgatg
     7921 accatttcca gcattatttc cacaaaactg actacagcgc agacaccaac cgcgtccgat
     7981 caaccgggag ccggtatgat gaaatggatg atgtatctga tgccggtgat gtttatgttt
     8041 atgttcaata attatgcatc aggtttgagc tactactatt tcttatctac gcttattact
     8101 attggacaga cttaccttat ccgcgcgact gttgacgaca aaaaactgct tgctcaactt
     8161 catgccaagg gtaacaaagc aaaaccaaaa aagaaaagcg gtttcatgga acgacttgaa
     8221 aaaatgcaac gtgaacagga aaaagcactg aaaaacagaa agtaaataac tccttcgtat
     8281 aaatttaatc ctttagccgc aaagctttac aaagaatttt ttaactttgt gaactttgcg
     8341 gctttacttt taaataaatt ccaagtgatg aaacgagcac atcaaatatt tattttgaca
     8401 cacgacttgt cccgatttgt cgggaaggag atgtttacat gccaattcca tgtgactatt
     8461 aaaatataaa ttatgaacac atgaaacaaa catgccaaca gtacttttaa cacatgactt
     8521 gtcccgatac atatcgggaa gggaatgatt atatggcgtg ttctccatat aaatagggac
     8581 aggctatctg acaataattt taaaaataaa aatataaaca gatgaaaaga ctgaattata
     8641 ttattttgct gatttctcta ttatttacat ttaccggctg tacaaagaaa agtcgtttcg
     8701 aaatcaatac ttcaaaagaa aaagtggatg ttaccataca ccggtttgac aaggatttaa
     8761 tcaatctgga tattagcaaa ctgaatcaat cagtggatgc attatacaaa acttattcgt
     8821 cgtttttgcc tttatatatt tataacgttt tggatacact acccgaagat acgctggctg
     8881 tagccggtat gatgaaacag ttcgtacagg atacatcttt tatttcggtc aacaaaaagg
     8941 tgatggaaac attcagcgat atatcagata ttgaaaaaga tatttccggt ggttttacgt
     9001 acattcacca ttatttccca aaactgccat tacctgaagt atattttttt gtttccggtt
     9061 tcaaccgttc ggtgctaatg acggacagta ttctcggagt gggaacggat ttttatctgg
     9121 gcgctgatta cgaaccctat aaatcattca cctacgatta tcttctccaa aatatgcggc
     9181 gcgaaagtct ggcaattgat gtggtttcgg ctacgctctt ccggtatttc agttttgatg
     9241 gaacgaataa tgccctgatc gataatatgc tgcaccgtgg taaggtaatc tatttattgt
     9301 ctgttttcat gcccggtaaa aaaatgaacg atatcattgg ctatacaact gaacaatgga
     9361 aatgggcaga acaatacgag gaggaaatat ggaaaacagt agttggtaag aaacatctgt
     9421 tctcaaccga tatgcagttg atcagtaaat acatgaacga tgcaccgttt acttcactcg
     9481 tttcacagga ttctcccgga cgcctgggaa cctggatagg atggaggata ataaaaagtt
     9541 atatggaaag gaatgaaaat gttacactgg aacagttaat gaaagagaat aattaccgga
     9601 aaatactgga acaatcgcat tacaaaccat aacgaaattt aatctgaata attaatggta
     9661 aaaatcctat ccggattttt accattatat gaaaactgaa aacctttttt gaccatggaa
     9721 gaacattatt tgtcgatacg aaactgggct gaggatgacc gtccgcggga gaaactcctg
     9781 aaaaaaggag taagtgcact gtcggatgca gaactgctgg caatcctgat tggttctggc
     9841 agcaaaaatg aaagtgctgt ggaactttgc cggaaaatac tcaaatcctc ccacgataac
     9901 ctgaacgaac tggctcgatt gtcaatcaat gagctgtgca gatttaaagg tatcggtgag
     9961 gcgaaagcag taactatttg tgcggcgctt gaattgggta aaagaagaaa aacaaacgaa
    10021 gtgcttgaac gaatgagaat aagtcagagt tcggatttgt acgaactatt tgaaccgata
    10081 ctgctggatt tgaaacacga agaattttgg gtggcctatc tcaacggtgc aaataaagtg
    10141 attgatgtga aaaaactgac acagggaggt tccaggcaaa ctgtggtgga tattcagatg
    10201 attttgaaaa tgtcactcga aaagtcagca caggctattg cagttgctca taatcatcct
    10261 tccggtcaga attacccaag ccgcgaagat gaaacaatca cacaacgcgt caaaaaagga
    10321 tgtgaagcca ttgatgttcg ttttctggat cacatcatca tagccggagg caaatatttc
    10381 agttttgccg acgaagggaa aatataatat ctacaaaaaa taatcgtaaa tgaatttatt
    10441 ggaagtcaac aatgtggtta agcagtattc tggtcagctg gcgctggatg gcgtaagcct
    10501 gactgtgcca aagggtactg tttacggctt attgggaccg aatggtgccg ggaaaaccac
    10561 gctgatccgc attatcaatt gcatcacagc gcccgatagc ggggaaatac tgctcaacgg
    10621 aaaaaaactt tcgccgaaag atgtacgcaa tattggttac ctgcccgaag aacgcggttt
    10681 gtataaaaaa atgaaagtgg gagagcaggc tatgtatctg gcacagctca aaggcatgac
    10741 aaagcacgat gcaaccaaag cattgaagta ctggttcgaa aaatttgaaa ttcaaagctg
    10801 gtggaacaaa aaggtggaag aactttccaa agggatggct cagaaagtac agtttatcac
    10861 caccatactc cataatcccg atttgctcat ctttgatgaa cctttcagtg gattcgatcc
    10921 tgtcaatgcc gatatgctga aaaaagaaat tcttgcattg cgcgataagg gtgcaacgat
    10981 tattttttcc acacacaata tggagtcggt cgaggaactt tgcgaaaata tttcgctggt
    11041 caacaaagct catattgttt tgcagggaaa tgttgctgaa attcgtgctt cgtattcaac
    11101 acgaactctc atggttcgtt ctgcggctcc tgttagctcc gatagctgtg agattgtttc
    11161 acaaaataag gtgaatggcg ttttcgaaac ttctctccgg atgattaagg aaatgtcaaa
    11221 taatgatttt ctgactgaac tgatgaaaca aaccgaaata cattcgtttg aagaattgct
    11281 ccccactatg aacgatattt ttatcagtac agttaagaat tcagaaaata taaataatcg
    11341 gctatgagta aaataggaat tataatcagg agggaatatt cctcccgtgt aatgaaaaag
    11401 tcctttttat tactgacttt tcttacgcca ctgttgtttg tggctatgat aatgatcccc
    11461 ctctggttat cgtctatcga agatgatacg ataaaaaata tcattgttat tgacaaaaca
    11521 ggcatctatc acgatgcgct aaaaagtaac gaaacctgta catttttgtt ttcggatgcc
    11581 ccgttggata attacaaacg gaaagataat aaggaaagtg aaatgactgc cgtgctctat
    11641 attaccgccg atctgaacaa gcaacccgat gccgtgaaaa tttactccga gaagcaggtg
    11701 aatgtcgagt taaaatctta catttccgga ctgctggaag attatattca gaaacaaaat
    11761 ctgaatgcct acaacatccc caacctgaaa gaacttgtcg aaaaatcaaa aacgaaattg
    11821 gatgttaaga caataaaatg ggatgccgaa ggtaaggaaa aggaggcttc tgccgaactt
    11881 gctttaatta tcgggatgat atcggcattt ctgatttata tttttatcat gtcctacggt
    11941 tcgcaggtga tgagcggtgt ccttcaggaa aagacaagcc ggattgtgga agtaatcatt
    12001 tcgtcagtaa aaccgttcga actgatgatg ggaaaaatta tcggaattgc gctggttggg
    12061 cttacccaat tctttctctg gatagctctt accctcatta ttagtgcagg agtgagcgcg
    12121 tttatgggcg gaagtttcaa tcctgaggca ttacaaacag ctcagcagat taatatgccc
    12181 ggaatgcaga caaatgttca gcaaactgaa actcaggcta tgatcgctga aatatataca
    12241 agcctgaatg gttttgattt tttcaaaacg ctggtgttgt tcattattta cttcctcgga
    12301 ggatatctgc tttacgcttc tctttttgca gccattggtt ctgctgttga caacgaaacc
    12361 gatacacagc agttctccat gccgctgaca atccccattc tgtttgccat ttatgcagcc
    12421 atctacagtg ctcaaaatcc cgatggtccg ctggcattct ggtgttcatt gataccattc
    12481 acttcaccca ttgtgatgat ggttcgtctg cctttcgatg tacccggctg ggaaatagtc
    12541 ttgtctgttt ttatcctgat actatctttt ataggtacta cctggatggc cggaaaaatt
    12601 tatcgtacgg gaatactgat gtatggcaag aaggtaagct ggaaagaact ctggaaatgg
    12661 ctgacgtata aataaaaaaa atcagaacct gttaaaggtt ctgattttaa tatgttttgt
    12721 agagaatgtg atgaaaaagg tttagaattt atgacgacct tcatcagcaa cagtaagttt
    12781 ggctctacct tttgcacgac gagcggctaa taccctgcgt ccgtttgcag aggccattct
    12841 ttcgcggaaa ccatgtttgt ttcttctttt tctttgtgaa ggttgaaaag tccttttcat
    12901 tgctattcgt atttaatttt attattttcg tttcttaaat tcggactgca aaaatagtga
    12961 ttttctttga atttacaaaa gaaactgtaa ttaaatttca gtttaatttc atgcaatctg
    13021 atttataaac agaaaaacca cgctgatcag gtgttcatgc ccccacatac gttgattacc
    13081 tggccgctta catagctcga caagtcactg gcaagaaaaa gtgcaacatt tgcaacttcg
    13141 tcgggggtac caccgcggcg caatggaatg gtttcagccc atttagcccg ttgttcatca
    13201 ttcagggcat gtgtcatttc ggtttcgata aagcccggtg caatagcatt ggcacggata
    13261 cctctggtgc caagttcttt ggcaatagat tttgccagac cgataagtcc tgctttggat
    13321 gcagagtagt ttgtttgtcc ggcattaccc gagactccta cgactgagct catattaata
    13381 atgcttcctg atttttgttt catcattatg ggtgtacatg catgaacaaa attgaatgcg
    13441 gatttcaggt tgacattaat aacagcatcc cactgttgtt cgctcatgcg catcattaaa
    13501 ccatctttgg taattccggc gttgtttacc agaatatcaa tgcgtccgaa atctttcaga
    13561 atttcttcta cgactttatg tgtatcgtcg taggatgctg cgtttgaagc atatcctttt
    13621 gcttttacac cgaatgcttc aatttctttc tgagtggtca gaccattttc atcgataacc
    13681 aaatcggtaa atgccacatg tgctccctcg gaggcaaact ttaatgcaat cgacttgcca
    13741 ataccgcgtg ctgctccggt tactatggct actttccctt ctaataactt catgttttaa
    13801 ttattttagg tgttaattat agattgcaaa gatacgcttt tttttgataa tgtacggaat
    13861 taaataattc acaaataaat gaagggaata aacctgagtt tattcccttg taaattagaa
    13921 cattagtcct gttaagcgga tttttaacgt tttcctctat ggatattaag actgtattta
    13981 tctgtttgag atgtcgcgtt tttgtagttt ttaatctttt gtatgcgtga tttaacaccc
    14041 tgtgatgtgt tttctttgtt taccaaacca agaatgccat cgtaatttcc cgtaatggtg
    14101 ttgcctagtt catcaatgaa cgtataattg agtatataat ttgagcctgt accttttatc
    14161 accgtcattg agccgcctgt aattggaaat gcatctgcag tatagtacca ggtgcccaaa
    14221 tcttctgatc cattgttgaa gccatctata acagtctgat tttgaactgt gttgattttt
    14281 acgggatatg ttccgttagg caatgatgtg gtgtatgatg tgggagtata aacttc
//
LOCUS       DAOPYB010000064        14307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4619_length_14307_cov_7.918257, whole genome shotgun sequence.
ACCESSION   DAOPYB010000064 DAOPYB010000000
VERSION     DAOPYB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14307
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4619_length_14307_cov_7.918257"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..443)
                     /locus_tag="PKH58_06635"
     CDS             complement(6..443)
                     /locus_tag="PKH58_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88740.1"
                     /translation="MLRQKLDNFHIYCHLVRQLIRKVLMYPLWNIMFSDKDNSDLAFN
                     ECYDLLYPSLLKMKVNDYPKHRKSPPKATSTHTPDYYADPLCVTHCVDQFSNIFRLYL
                     RRYFNKHRLMRQSLARKIKSFFKRWNDTSNHIFDLLAAFRKAA"
     gene            1422..2606
                     /locus_tag="PKH58_06640"
     CDS             1422..2606
                     /locus_tag="PKH58_06640"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNX88741.1"
                     /translation="MEKEIVIVSAVRTAVGNFNGALSGFSAVDLGVIAAREAISRAGI
                     QPSDVDETIIGNILSAGLGQNVARQISVKSGVPVTSPAMSINILCGSGLRAVSLAAQM
                     IMVGNSEIVLCGGTESMTNAPYLLPQARSGYRMGDGKLIDSMIKDGLTDAFNDYHMGV
                     TAENIAEKWNISKEEQDEFAVRSQNKCEAAQKAGKFADEIVPISIPQRKGDPIIFDTD
                     EFPRHGATIEQMAKLKPAFKKDGTVTAANASGINDGAAMLIVMSREKADALGLKPLVK
                     IKSFGTTALEPEIMGYGPVSATQKALQQANLTIDDIDLAELNEAFAAQSIAVVRDLKI
                     NPDIVNVNGGAIAIGHPVGASGARILTTLIYEMKKRQSEVGLASLCIGGGQGTAVIVE
                     NT"
     gene            2733..3473
                     /gene="fabG"
                     /locus_tag="PKH58_06645"
     CDS             2733..3473
                     /gene="fabG"
                     /locus_tag="PKH58_06645"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNX88742.1"
                     /translation="MRLKDKIAIVTGGADGIGKATAVKFAAEGATVIIWDLNTDKGTE
                     TVKEITENGGKVSFAKVNTSVYAEVEAATKDVVEKFGTLDIIINNAGITRDASIKKMT
                     PELWQQVIDVNLTGVFYCCKCASEVMVEKGYGRIINASSVVALYGNFGQTNYVATKAG
                     LIGMTKTLARELGRKGITVNAVAPGFIATEMVKKMPPEVLKGMEEKVPLKRLGQPEEI
                     AAAYLFLASDEAAYINGTVLSVDGGITI"
     gene            3484..5040
                     /locus_tag="PKH58_06650"
     CDS             3484..5040
                     /locus_tag="PKH58_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88743.1"
                     /translation="MATSAQVVGASILFAVYGIFILYFVVKGSRGTKSINDYAVGSFT
                     FSPVFVALSLAAAMTSAATFVINPGLIATYGLSGYLSFGIVFPVASMVSLVVLTKSFR
                     KYGQSMSAVSLSSWIGKRYESKGYALFIAFLTFLLITFIVLILVALVKVISQALNADQ
                     ILVLGLLIAFVFGYMMFGGANSMVYTNTIQAFIMVLVAIILIGSGIQFFKDGFGAFFE
                     KLRLIDPVLVKPTNPQSPLFRDLFEIIFVQMIVGAAVVCQPHIITKSLLLKKESDVNK
                     FLVVAVIVQMLFYSVVVAGLYARLKFPDLTQNGAHLNVDSIISAYVIEVFSGGFASLL
                     VGLLVILGLISAGFSTIEGLIQSLSTTITTDIIKPLFGKNINENNYVKVNRLVILFLA
                     IISFFVARDQIIQPNLSVAIFAQNGVYAYFSILFVPIVFGIFLKNVNFITAFSGSLTA
                     LIVYYSVYYLFPALLKSGAADFGFFNHYFTGNVQNPAVAASIAILMSFVVGFLVHLTK
                     KKKLAANNLQ"
     gene            5046..6356
                     /locus_tag="PKH58_06655"
     CDS             5046..6356
                     /locus_tag="PKH58_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNX88744.1"
                     /translation="MNNFKDLYKRKLIPLDKMPDLLQSDTDVIVAQCASEPQGCMSVF
                     HLAKDRVRDVKVFSVLTLKPYDFYMKPEMKGHFELCSWFHAPGSRKALKEKTGTVTYV
                     PNMLHRAASDRMKVRRPHMFVGTCTPPDEKGFVSLSLGLTYEKDILEVAEISVLEVND
                     QLPRTLGDTQVHISDVSYFVEYSQMPPTLPEPAPDAVAMAIGKNIADLVDDGSTIQLG
                     IGEIPNAAALMLKDKKDLGVHTEMMVDSMMELYEMGVITNKAKSIHKGKFICTFAMGS
                     EKLYKWLDNNQAVEFLRGSYVNDPCFMRQNSKMVSINTCIMIDFTGQVASESIGIEQY
                     SGTGGQSDTAVGATEGLDGKGKSIIACRSTAKRDTVSTIVPVLPAGTGVTLHRSNTDW
                     VVTEFGSVRLTGRTVRERTEMLISVAHPDFREELRKQAEEMGYL"
     gene            6519..7526
                     /locus_tag="PKH58_06660"
     CDS             6519..7526
                     /locus_tag="PKH58_06660"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNX88745.1"
                     /translation="MLPIKIVSTGLYAPGEPISNEELKKLAGIEFDEEKIEGKIGIKH
                     RHIAHFRGLEETTADFVTHAAENALKNAGLSADELGLIIVATDTPEYVTPATAIIVQG
                     RLQKGENWCMSFDVSASCASFTMAFDTAARILATNPKINYALITGVYNMPAFIRPGDK
                     FSYPIFADGAASFILTKDENGSSGYIGNQMLTDGTQFDYIGIYAGGSKMPVTNEILQE
                     EKNGLLSLKPLPGDRNVRLWPMVVEKLLKEYNTNADEIDHYIFTQINRSVILKVMAEI
                     NQPEEKAMMVMDRYGYTGSACVPMAFHEGVKSGKIKRGDKVLFMASGAGLSVGSNLVV
                     Y"
     gene            7662..8675
                     /locus_tag="PKH58_06665"
     CDS             7662..8675
                     /locus_tag="PKH58_06665"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNX88746.1"
                     /translation="MTNVGIVGTGIYIPEKRMTAADISVATNGVWSETAVIEKLGIVE
                     KPVPGTEDGTQEMGVRAALDCLKNTGIDPLEIDLILCIGEEWKEFPLTTSGIYIQYKI
                     GAENAWALDIQQRCCTTVAAMKIAKDMIIADETLHTVMIVGGYRNGDFIDFEDSAVSF
                     MYNLSAGAGAMIVKRNYNKNLILGTHIVTDGSLARAAGVKYGGTENPINTENHHLAYK
                     SLKVFDEKFMKDRLNEVSMKNWFYCIDKAFEKSNIRKNELGFLCCLHFKKSMFDYMLT
                     ELGLTSDQSIYLDKYGHMGQIDQILILHLAQQQGKLKDGTIISMIAAGIGYAWGANVI
                     RWG"
     gene            8839..9342
                     /locus_tag="PKH58_06670"
     CDS             8839..9342
                     /locus_tag="PKH58_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88747.1"
                     /translation="MKKLFFILISVALVFSACKKSEETEVYDPATDGLIGNWLSKGAD
                     VAPLLKTYFAIDSITAMFKADNTYLVKSYSKGAATTYSGTYLQTKPATGTIWTIKLNQ
                     STPTAVTSEGIFEVAGTSPNFTLKYEVVQTEPSIGATPPTITAGFGSSNGGALLQMNV
                     QKFVIVK"
     gene            9431..11026
                     /locus_tag="PKH58_06675"
     CDS             9431..11026
                     /locus_tag="PKH58_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88748.1"
                     /translation="MKKIIGSLILFLITTTVAFSQLPTNQYFSKEIPDKANKEFQFLG
                     FFINQAVTSNIYPENDFLKGQVVGRLFGGNTSKTSNSHTSAYVEQRFLPFFIYTPNLF
                     NGKATLRASFELDWTWGDVAYGVGGNFGGGFSADQVNLQTQNLELELNPTKGLYVNLG
                     LQRIFDTPYNPYRTLFDKMTYTGYRLAYFGTDGVGVSVRKDWDFARMKGGYYKLYENY
                     VHLNDDVTLMELNGEKDVSRTWKIGGSAYYMRDRANGAGGVSILSQGFNSLLASHNGV
                     YRFNYGGAKYRGDVGWIGTYFSRNADQWLDPWTLTGFVNVNIGRADTLKNQLISDKEW
                     TKATDILGFAANVRGSYRYGQTQNDNVSLDIMYSSGDANGLKDGKYNGVITGNQWGAP
                     GAIFISSGAYLLMPHGNVVNRFTPAIMDISNMGYGMTSATLNASHGFIPNKLNAKIGT
                     AAAISNVQPLGGGSLIGAEVNGNIQYNFGPFMSLELHGAYLMLGDFYDSKQSAYGSPV
                     NGNIDGRPVNPWTAFMVFKWLLF"
     gene            11045..12010
                     /locus_tag="PKH58_06680"
     CDS             11045..12010
                     /locus_tag="PKH58_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX88749.1"
                     /translation="MRKKIIYILIAAISTVLSSCSTPYHSITNIKSFDEMQYKYPVKK
                     IHLPVSGFDIAYTDEGKGENTIIFIHGLGSYLRAWEKNIGELSKTNRCIAIDLPGYGK
                     SSKQPHSGMMTFYAGIVNELVTELKLQKVLIAGHSMGGQIAMTTALVYPEVVKGLILA
                     APAGFEPFTKGQKQWFKEVMTLDGVRLTTPEAIQNNLATNFYRVPKDADFMITDRIAM
                     RSASDFKAYCYAVVQSVHGMVDNPVLDYLLDIKVPTLILFGENDNLIPNRYLNPGRTI
                     EIAKNGASKIPNNKLVMIPRCGHFLMFEKPEIFNSETLKFLDSVK"
     gene            12254..13531
                     /locus_tag="PKH58_06685"
     CDS             12254..13531
                     /locus_tag="PKH58_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88750.1"
                     /translation="MPKSFDTEEDKRTYNYLKTWITDTLAKIDVDLYFEWLISTEKKV
                     LTDIIVPADEVVLLNNMKKIGPTNYNFIRFYELAQYYRDYLMVRNRTKYNKVVSEFLS
                     KYHEQYHLLKKINLELDEVTAEIVKKEEFSEDESSYIENFLHTIYFNESLDAYTRYRA
                     VVRLTIYYYNNRQFQKQAVVYQHLDEMLKTPLFYSKRILANYYANRAMMHSKLNELAQ
                     SEKYGYLSIQNKNSDYLFYLINLCGVLLKQGKNEEALKLMRKSMPELKNTNNNYYKIG
                     FNSFYIRTLIYNGQSEKAVEYAAHYFDAYKKEIFEHRWHLFFRSYIHALISTEKYGKI
                     LSLCKRYKLLTKEKHRIERIDYLPIIQSYYYLAEYMESIISKEKLISSIIKTYTNLMD
                     DNYRSQKINELLSELMPNIPEEIKVIKKSLILN"
     gene            complement(13538..>14307)
                     /locus_tag="PKH58_06690"
     CDS             complement(13538..>14307)
                     /locus_tag="PKH58_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNX88751.1"
                     /translation="RPTGLLDPVIDVRPSLNQIDDLLEEITLRTEAGERVLVTTLTKR
                     MAEELTEYLLRMNVRCNYIHSDVTTLDRVQIMEDLRRGVYDVLVGVNLLREGLDLPEV
                     SLVAILDADKEGFLRNHRSLTQTAGRAARNLNGKVIMYADKITMSMQKTIDETNRRRE
                     KQLAYNAKFGITPTQITKGERNSLSQMEPNAYIEPEIPNILAADPVVQFMSRPALEKA
                     IAKARRSMQEAAKKLDFLEAAQYRDEMIKLEELLKGK"
BASE COUNT         4368 a         2748 c         3089 g         4102 t
ORIGIN      
        1 ataaattatg ccgcttttct gaaagccgcc aataaatcga aaatatggtt cgaggtatca
       61 ttccatcgtt tgaagaatga ttttatcttg cgggctaatg actgacgcat cagccggtgt
      121 ttgttgaagt accgccgcaa gtaaagccgg aatatgtttg aaaactggtc gacacaatgg
      181 gtgacgcaaa gtggatcagc ataataatcg ggtgtgtgag tgcttgttgc ttttggtggt
      241 gattttctgt gtttcgggta atcattcact ttcattttca acaaggaagg atatagtaaa
      301 tcgtagcatt cattaaatgc cagatcggaa ttgtccttat cgctaaacat aatattccaa
      361 agcgggtaca tcagcacctt ccttatcagt tgtctgacaa gatggcagta aatgtggaaa
      421 ttatcaagct tttggcgtaa catgtttatg aattaagaaa tttaataact gttatcatac
      481 cacaataatc gtgccaatcg ctaaatatca ttaaatatca ttaaatcgat gattaaatgg
      541 cgggaaaccg gacagaatag cagaaaacgg aaggagaatt aggcagtgta gagacggagc
      601 gctgccagtg ggtttgagat gttaagccaa actgagcttg ttttaattaa ttatgaatat
      661 ccgtttggtt aactaatctt ttagaattaa gacatcaggg ctacgcaccg aaatatcgat
      721 tttacaatca tcgtgctact aacctgcctg cagcaggtag ggatatcaca gctatgctgc
      781 tccaaatatc tctggtttag gggcattagc cctgatgtct ttgtaggata ttattcacag
      841 aataatcaaa ggaacgtagc tctgtcatct atatttttat aattaactca acacacctca
      901 ctctgattgt tttattaaaa aaaatcattt cactgtacaa ataataaaac catttaagtt
      961 tccaaataaa aacagcaata ttcctcacat aatgtatcgg tgcgctgcat ctgaatataa
     1021 gccatagagt ggcgtaacat ttgatgaaaa ccgataatcc ccgaatttta aggtacagcg
     1081 taccgaaata tttttaattg agcttttcag gagaccctaa tcaggtaaca attaacaaaa
     1141 gaaaccggga aaacccgaag tggtttctga attagctcct ttcgattcaa aatccacaga
     1201 aaagccgata tattcatttt ttgaagatat cggcttttat ataattaatc aggttggttt
     1261 taagtcaact catctattta tattcatatt ctattgatat tcttgatatt aatacaacat
     1321 agatgcaatt tgatattcat aagtcaatat gtgaatatat tttttcttat attcagtacc
     1381 atttttttct tacgtatttt tgagcaaaaa tcaaatatat catggaaaaa gaaatagtta
     1441 tagtatcagc agttcgtaca gccgttggta atttcaatgg tgcattatcg ggtttttcag
     1501 cggttgattt gggtgtaata gcagcccggg aagccatcag cagagcaggc attcaacctt
     1561 ctgatgtaga tgaaaccata attggaaata tcctgagcgc aggtttaggt caaaatgtag
     1621 cccgtcagat atcggtgaaa agcggcgttc ctgtaaccag tcctgccatg tctattaata
     1681 ttttatgcgg ctccggctta cgggctgttt ccctggcggc ccaaatgatc atggttggta
     1741 attccgaaat tgtactttgt ggaggaaccg aaagcatgac caacgcgcca tatctgcttc
     1801 cgcaagcccg gagcggctac cgcatgggcg atggaaaact gattgacagt atgattaaag
     1861 acggacttac agatgcattc aacgattatc acatgggcgt tactgccgaa aatatcgccg
     1921 aaaaatggaa catcagcaaa gaggagcagg atgaatttgc ggtgcgtagt cagaataaat
     1981 gcgaagccgc tcaaaaagcc ggaaaatttg ccgacgaaat tgtgcccatc tccatccctc
     2041 aacgcaaagg cgaccccatc atttttgaca ccgacgaatt tccacgtcac ggagccacca
     2101 ttgagcagat ggcgaaactg aaaccggctt tcaaaaaaga cggaacagtg actgctgcca
     2161 atgcatccgg cattaacgac ggtgctgcca tgctcatcgt aatgtcacgc gaaaaagccg
     2221 atgcgcttgg cctgaaaccg ttggtaaaaa tcaaatcctt cggcacaact gctctcgaac
     2281 ccgaaataat gggatacgga ccggtttcag ccactcaaaa agcgcttcaa caggcaaatc
     2341 tcacaattga tgatattgat ttggccgaat tgaacgaagc atttgccgct caatccatag
     2401 ctgtggtgcg cgatctgaaa atcaatcccg atatcgtaaa tgtgaatgga ggtgcgattg
     2461 ctatagggca tccggtgggt gcttcgggtg cacgaattct caccaccctg atttatgaaa
     2521 tgaaaaaacg tcagtcagaa gtgggactgg cttcgctgtg tatcggcggc ggacagggaa
     2581 cagccgtaat tgtggagaat acataaaccc ctaatcctta aagggaaatt taaagtatta
     2641 acatacaaat tttatttaat atttatcaaa aaaataactt acacctccaa tcaaaactcc
     2701 cctttagggg ttgggggtaa atatttatta tcatgagact taaagacaaa attgcgattg
     2761 taacaggtgg tgccgacgga ataggcaaag caaccgccgt aaaatttgct gccgaaggcg
     2821 caacggttat tatttgggat ctgaataccg ataaagggac agaaactgta aaagaaatta
     2881 ccgaaaacgg tggaaaagtg agttttgcaa aagtaaacac ctcggtgtat gccgaagtgg
     2941 aagctgctac taaagatgtg gtggaaaaat tcggtacatt ggatattatc attaataatg
     3001 caggaattac ccgcgatgct tccatcaaaa aaatgacgcc cgaactgtgg cagcaggtaa
     3061 ttgatgtgaa cctcaccgga gtattctatt gctgcaagtg tgcatcagaa gtgatggtgg
     3121 aaaaagggta cggacgaata atcaatgctt catcggtagt agccctttat ggtaatttcg
     3181 gtcaaacaaa ctacgttgcc actaaagcag gtttaatagg gatgacaaaa actcttgcac
     3241 gcgaattagg tcgtaaaggt atcacagtca atgccgttgc tccgggattt atcgccaccg
     3301 aaatggtaaa aaaaatgccg cccgaagtat tgaaaggcat ggaagaaaaa gtacctttga
     3361 aacgcctggg tcaacccgaa gaaattgctg cagcctatct tttcctggcg agtgacgaag
     3421 cggcctacat caacgggaca gttttgagcg tcgatggcgg tataactatt taataatcaa
     3481 cttatggcaa catctgcaca agtggtaggc gcttccatac tttttgccgt ttacggaata
     3541 tttattctgt attttgtggt aaaaggttcg cgcggcacaa aaagtatcaa cgactatgca
     3601 gtcggcagtt ttacattttc gccggtattc gtagcgcttt cgctggctgc agctatgacc
     3661 agcgcggcta cattcgtcat aaatcccgga ttgatagcca cttacggttt gagcggttat
     3721 ttgtcatttg gtattgtatt tcctgttgca tccatggtgt cgctggttgt gctgactaag
     3781 agtttccgaa aatacggtca atcgatgagc gcagtttcac tttcgagctg gattggaaaa
     3841 cgctacgaaa gcaaaggata cgcacttttt attgctttcc tcacatttct gctcattact
     3901 tttattgtgc tcatactcgt tgcactcgtt aaggtaattt cgcaggcact caacgctgat
     3961 caaattctgg ttttaggttt actgatagcg tttgtatttg gatatatgat gtttggcggt
     4021 gccaattcaa tggtttacac caataccatt caggcattta ttatggttct ggtggcaatt
     4081 attctgatag gatccggcat tcagtttttc aaagatggtt tcggagcttt ttttgaaaag
     4141 ttacgattaa ttgaccctgt ccttgttaaa cctaccaatc cgcaaagtcc gctgtttcgc
     4201 gatttattcg aaattatatt tgtgcagatg atagtaggag ccgccgttgt ttgccagccg
     4261 catatcatta caaaatcact tttgctgaaa aaagaaagcg atgtgaacaa atttcttgta
     4321 gtagctgtaa ttgttcaaat gcttttctac tctgtagtgg tggccggatt gtatgcaagg
     4381 ctcaaattcc ccgatttaac tcaaaacggt gctcatctga atgttgacag catcatttcg
     4441 gcctacgtaa ttgaggtttt ttcaggtgga tttgcttcac tcctggtagg tttgcttgtc
     4501 attctggggc tgatcagtgc cggattttcc accatcgaag ggctcatcca atcgctgagt
     4561 accactatca ccaccgacat cattaagccc ttgtttggga aaaacatcaa cgaaaataac
     4621 tacgtaaaag ttaaccggtt agtaattctg tttttagcaa taatttcatt ctttgtggca
     4681 cgagatcaga ttatacagcc aaatttaagt gtggctattt ttgctcaaaa cggcgtttac
     4741 gcctattttt ccattttatt tgtacccatt gtgtttggta tatttctgaa aaatgtaaat
     4801 ttcattaccg ctttctcagg ctcgctcacg gctttgattg tgtactactc ggtgtattat
     4861 ttgtttccgg ccttactgaa atcaggcgct gccgattttg gtttttttaa tcattacttt
     4921 accggcaacg tacaaaatcc ggcagttgca gcctcaatag caatcctcat gtcatttgta
     4981 gttggatttt tagtacattt gacaaaaaag aaaaaattag ctgcgaataa tttacaataa
     5041 acagtatgaa taactttaaa gatttatata aaagaaaact cattccgctg gataaaatgc
     5101 ccgatttgct tcaaagcgat acggatgtga tagtagcaca gtgtgcttcg gagccgcagg
     5161 gttgcatgtc ggtatttcat ctggccaaag accgggtgag agatgtaaag gttttctcgg
     5221 tgctgaccct caagccgtat gatttttaca tgaaaccgga gatgaaagga catttcgaac
     5281 tgtgcagctg gtttcatgca ccgggttcac gcaaggcgct gaaagaaaaa accggaacgg
     5341 taacctatgt gcccaatatg cttcacaggg cagcttccga ccgtatgaaa gttcgccgtc
     5401 cacacatgtt tgtgggcacg tgtactcctc ccgacgaaaa aggttttgtt tcgctttcgc
     5461 tgggacttac ttacgaaaaa gacatactgg aagtggccga aatttcggta ctcgaagtga
     5521 atgaccaact gccccgcacg ttgggcgaca cacaggttca catttcggat gtgtcttatt
     5581 ttgtggaata ctcccaaatg ccgcctacat tgcccgaacc tgcacccgat gcagtagcaa
     5641 tggctatcgg gaagaatata gccgacttag tagatgacgg atcgaccatc caactcggaa
     5701 ttggcgaaat tcccaatgca gctgcattaa tgctgaaaga caaaaaagac ctgggagttc
     5761 ataccgaaat gatggtggac agcatgatgg aactgtatga aatgggggta attaccaaca
     5821 aagccaagag tattcacaaa ggcaaattca tttgtacatt tgccatggga agcgagaaac
     5881 tctacaaatg gctcgataat aatcaggcag tggaattttt acgtggcagt tacgtaaatg
     5941 atccttgttt tatgcgtcaa aactccaaaa tggtttcaat caacacctgt attatgattg
     6001 attttaccgg gcaggtagcg agtgaaagca tcggtataga acaatattca ggtacaggag
     6061 gtcagagtga tacggctgta ggtgctactg aaggtctgga tggaaaagga aagtcaatca
     6121 ttgcctgccg ttcgactgct aaaagagata ctgtttcgac tatcgtaccg gtactgcctg
     6181 cagggacagg cgtaacatta caccgcagca ataccgactg ggttgtaact gagtttggaa
     6241 gtgtccgcct gaccgggcgg accgttcgtg agcgtaccga aatgcttatt tcagtagcac
     6301 atcccgattt cagggaagaa ctcagaaagc aagccgaaga aatgggttat ttgtagaaaa
     6361 gccccccggc cccctgaagg gggagtagag tgtgaggcaa tcttacattt tctggaataa
     6421 gaaagtttat ttttcaaatt aaataaatat ttaagtttta cacaacgctc tattaacttt
     6481 accttcagag agttagggtg ggcagatata ttttcaagat gttaccaata aaaatagtat
     6541 ccacaggact ttacgctccg ggtgagccga taagcaacga agagttgaaa aaactcgccg
     6601 gaattgagtt cgacgaagaa aaaatagaag gaaaaatcgg aatcaagcat cgccacattg
     6661 cccatttcag gggattggag gaaacaactg ccgattttgt aactcacgca gctgaaaatg
     6721 ctttgaaaaa tgccggatta tctgccgacg aacttggatt aatcatcgtg gccaccgata
     6781 ctccggagta cgttacgccg gctacagcta ttatcgtgca gggacgtttg cagaaagggg
     6841 aaaattggtg catgagtttc gatgtttcag catcatgtgc cagtttcacg atggcatttg
     6901 acacggcagc gcgcattctg gctaccaatc cgaaaatcaa ttacgcactg attaccggtg
     6961 tgtataatat gcctgctttt attcgtccgg gcgataaatt ttcttatccg atttttgccg
     7021 atggggctgc gtcgtttatt ttaactaaag atgaaaatgg ttcttctggt tatattggaa
     7081 atcagatgct caccgacgga actcaatttg attatatcgg aatttatgcc ggaggttcga
     7141 aaatgccggt tacaaatgaa attttgcagg aagaaaaaaa cggattactg agtttgaagc
     7201 cgttgccggg cgaccgaaat gtgcggctgt ggcctatggt agttgaaaaa ctgcttaaag
     7261 aatacaacac caacgccgat gaaattgatc attacatttt tactcaaatc aatcgttcgg
     7321 taatcctgaa agtaatggcc gaaatcaatc agcccgaaga aaaggcgatg atggtgatgg
     7381 atcggtacgg gtacacgggt tcggcttgtg ttccgatggc ttttcacgaa ggagtaaaat
     7441 ccggcaaaat caaacggggc gataaagtgc tttttatggc atccggtgca ggtttgtcgg
     7501 taggtagcaa tctggtggtg tattgaaata gtgccaatcc ccctaacccc ctgaaggggg
     7561 gattttggtg gcttttggga aaattgtaaa aaaataaata taaatcaaac agaataaaac
     7621 tattaaaatt cccccttcag ggggttaggg ggcatatatt tatgacaaac gtaggaattg
     7681 taggaacggg aatttatatt cctgaaaaaa gaatgactgc tgccgatatt tcggttgcta
     7741 ccaacggtgt gtggagcgaa acggcagtga tagaaaaact cggaatagtg gaaaaaccgg
     7801 tgccgggtac tgaagacggt acccaggaaa tgggagttcg ggcagcactg gattgcctga
     7861 aaaataccgg aattgacccg ctggaaatag atttgatact ttgcattggc gaagaatgga
     7921 aggaatttcc attgaccact tccggaattt acattcagta taaaataggt gccgaaaatg
     7981 cgtgggcgct tgatattcag caacgctgct gtaccactgt ggcagcaatg aaaatagcca
     8041 aagacatgat tatagccgac gaaaccctgc atacagttat gattgtgggt ggttaccgaa
     8101 acggcgattt cattgatttt gaagacagtg cagtttcatt catgtacaac ctttcggccg
     8161 gtgccggagc catgattgta aagcggaact ataacaaaaa cctgatttta ggaacccaca
     8221 tagtgaccga cggttcgctg gcacgggctg ccggtgtaaa atatggcgga acagaaaatc
     8281 cgataaatac cgaaaatcat catctggctt acaaatcatt aaaagttttt gatgaaaaat
     8341 tcatgaaaga ccggttgaat gaagtttcta tgaaaaactg gttttattgc attgataaag
     8401 catttgaaaa atcaaatatc cgaaaaaacg agttaggttt cctttgttgt ctccatttca
     8461 agaagagtat gttcgattac atgctcacag agctcggact tacatcagac caaagcatct
     8521 atttagataa atacggtcat atgggacaaa ttgaccagat tttaattcta catttggctc
     8581 agcaacaggg aaaacttaaa gatggtacaa ttatttcaat gattgcggct ggaattggct
     8641 acgcctgggg agccaatgta atccggtggg gataaaaatc aacgaataaa tataaacatc
     8701 taatagtctt ataatcaatt aataatatca tatatttgaa attacaatac attagtcaac
     8761 cccttttatt tttatatttc aacattaatc aattttagaa ttcacaaatt tgtaataaca
     8821 attaaaaatt tatttatcat gaaaaaatta ttttttattt tgatctcagt tgcattggtt
     8881 ttcagcgcat gtaaaaaaag tgaagaaaca gaggtatatg acccagcaac agacggtttg
     8941 attggtaact ggctttcaaa aggcgcagat gttgcccctc ttttgaagac ctattttgcc
     9001 attgactcca ttacagctat gtttaaggct gataatacgt atctggttaa gtcctattct
     9061 aaaggagctg ccaccactta ttcaggcact tatctgcaaa caaaacctgc tacaggtaca
     9121 atctggacca ttaaattaaa ccaaagtaca cctacagcag taaccagtga aggtattttt
     9181 gaagttgctg gtacaagtcc aaatttcaca ctgaaatatg aagttgtaca aacggaacca
     9241 agtattggtg caactcctcc aacaataaca gccggatttg gcagcagcaa cggtggagct
     9301 ttgttacaaa tgaatgttca aaaattcgtt attgtaaaat aatttgtttg tctttaaaaa
     9361 tcagggtgta ttgaaaatat atcctgattt ttcttttatt gatttgataa tccaatttaa
     9421 ttaaataaaa atgaaaaaga taattggctc attgatatta tttttaatta ctacaacagt
     9481 tgctttttca caattaccta ctaatcaata tttctcaaag gaaattccgg ataaagctaa
     9541 caaagaattt cagtttctcg ggttctttat taatcaggct gttacttcaa atatttatcc
     9601 cgaaaatgat tttttgaaag gccaggtagt aggacgattg tttggtggca atacatcaaa
     9661 aacgagtaat tcacacacga gtgcgtatgt ggaacaacgg tttttgcctt tcttcattta
     9721 cactcctaat ttattcaacg ggaaagccac actgcgtgcc tcttttgaac tggactggac
     9781 ctggggtgat gtagcttatg gagtgggtgg aaattttgga ggtggattct cggccgatca
     9841 ggtaaacctg caaacccaaa atctggagtt ggaattaaat ccaacaaaag ggttatacgt
     9901 gaacttaggt ttgcaacgga tattcgacac accctacaat ccctatagaa ctttattcga
     9961 taagatgacc tacaccggtt atcgtttagc ctatttcggc accgacggtg taggtgtttc
    10021 tgtacgtaaa gactgggact tcgcacggat gaaaggcgga tattacaaac tatatgaaaa
    10081 ttacgtgcac ttaaatgacg acgtgacgtt gatggaactt aacggggaaa aagatgtgag
    10141 ccgcacttgg aaaattggtg gttcagcgta ttatatgcgc gatcgggcaa atggtgcagg
    10201 aggtgtttct attctcagtc agggatttaa cagtttgctg gccagccaca acggggtata
    10261 cagattcaac tatggaggtg caaaataccg tggcgatgta ggttggattg gtacatactt
    10321 tagccgcaat gcagatcaat ggctcgatcc gtggacattg accggatttg tgaatgtaaa
    10381 tataggtcga gcagatacac ttaagaatca actgatttcc gacaaagaat ggaccaaagc
    10441 aactgatatt cttggttttg cagccaacgt gagaggttcg taccgctacg gacaaactca
    10501 gaatgacaac gtttcgctgg atattatgta ttcatcgggc gatgctaacg gattgaaaga
    10561 tggaaaatac aacggcgtaa ttaccggaaa tcagtggggt gccccgggtg caatttttat
    10621 cagttcgggt gcttacctgc tcatgccgca cggaaatgta gttaaccgct ttacgcccgc
    10681 tattatggac atttccaata tgggatatgg catgacttcg gccacactca atgcttcgca
    10741 tggatttatt cccaataaac taaatgcaaa gattggaact gcagcagcta tcagcaatgt
    10801 acaacctctg ggtggaggtt cactgattgg ggctgaagtg aacggcaata ttcaatacaa
    10861 tttcgggccc ttcatgagtt tggaactcca tggtgcctat ttgatgctcg gagatttcta
    10921 cgacagcaaa caaagcgcct acggaagtcc ggtaaacggg aatattgacg gacgacctgt
    10981 taatccatgg actgctttta tggtgtttaa atggttacta ttctaacaat attaatttac
    11041 gattatgaga aaaaaaatca tttatatatt gattgcggca atttcaacgg tattaagcag
    11101 ttgcagcaca ccataccact ccatcaccaa cataaaaagt tttgatgaaa tgcaatataa
    11161 gtatcccgta aaaaaaattc acttacctgt ttcgggattt gatattgctt acaccgacga
    11221 agggaaaggt gaaaatacca ttatttttat tcacggcctg ggaagttacc tgcgtgcctg
    11281 ggaaaaaaac attggtgaat tgagtaaaac caaccgttgt attgctattg atttgcccgg
    11341 ttacggaaaa tcgagcaaac agcctcatag cggtatgatg acattctatg ccggtattgt
    11401 caatgaatta gtcactgaat taaagttgca aaaggttctc attgccggac attccatggg
    11461 tggacagata gcaatgacca ccgcactggt ttatcctgaa gtagttaaag gattaattct
    11521 agcggcaccg gcaggttttg agccatttac aaaaggacaa aaacagtggt ttaaggaagt
    11581 tatgacattg gatggcgtgc gtctgacaac accggaagca atccaaaaca acctggctac
    11641 taatttttac cgggtaccca aagatgccga ttttatgatt accgaccgta ttgccatgcg
    11701 tagtgcaagt gatttcaaag cttattgtta tgctgttgta caatcagtac atggtatggt
    11761 tgacaatccg gtgttggatt atctgctgga tatcaaggtg ccgacactta ttctttttgg
    11821 tgaaaatgac aatctgattc ctaaccgcta cctaaatccg ggtcgtacaa tcgaaattgc
    11881 caaaaacgga gcctcaaaaa ttccgaacaa taaattggtg atgattcccc gttgtggtca
    11941 ttttctgatg ttcgaaaaac ctgaaatttt caattctgaa acattaaagt ttcttgattc
    12001 tgtaaaataa gttttcatga tgaaatgaga aagagaaaac ggaatcaatt ctgttttctc
    12061 tttctttttt gtatttttat ggcttaaatc aaattctata attgagatgc gaaaaaaact
    12121 gaatacattt tatctgtttt ctaatactct ttatccgcat gaattggagt acttactttc
    12181 aatacagagt tttaataaac ctgttaatct aaacatcctt aaacaaatat atatcaattc
    12241 tacttcgtca caaatgccta aatcatttga taccgaagaa gacaaaagga cctacaacta
    12301 tctgaaaacc tggattacag atacattggc aaaaatagat gttgatttgt atttcgagtg
    12361 gttgatctcg accgaaaaaa aagtacttac cgatattatt gtgccggctg atgaagttgt
    12421 attgctcaat aatatgaaaa aaatagggcc aacaaattac aattttataa ggttttacga
    12481 acttgcacaa tactaccgtg attacctcat ggtacgcaac agaacgaaat ataacaaagt
    12541 tgtatctgaa tttctgagta aatatcacga acaatatcat ttgttgaaaa aaataaatct
    12601 cgaactggat gaagttacgg ctgaaatagt gaaaaaggaa gaattttcag aagatgaaag
    12661 ttcatatata gagaattttc tgcacaccat ttattttaac gaaagtctgg atgcctatac
    12721 ccgatacagg gcagtagtgc ggttgaccat ttactattat aataaccgtc agtttcagaa
    12781 acaagcagtt gtgtaccagc atctggatga aatgctgaag acaccgctct tttactcaaa
    12841 acgcattctg gcaaattatt atgccaacag ggcaatgatg cattcgaaac tgaatgaatt
    12901 ggctcaatct gaaaagtacg gttacctttc cattcaaaac aaaaacagtg attatctatt
    12961 ctacctgata aatttgtgcg gggttttgct taaacagggt aaaaatgagg aagcactgaa
    13021 attgatgcgt aaatcaatgc cggaattgaa aaataccaat aataattatt ataaaatcgg
    13081 ctttaactca ttctatattc gtactttgat ttacaatggg caaagtgaaa aggctgtgga
    13141 atatgctgca cattatttcg atgcttacaa aaaagagatt tttgaacacc ggtggcatct
    13201 tttttttcgg tcatacattc acgcactaat ttccactgaa aaatacggta aaatattatc
    13261 gctttgtaaa cgatataaat tgctgacaaa agaaaaacat cgcatcgagc gtattgatta
    13321 tctgcccatt atacaatcct attattatct ggctgaatat atggaaagca ttatttcaaa
    13381 agaaaaactg atttcatcca ttataaaaac atatacaaat ttgatggatg acaactatcg
    13441 ttctcagaaa atcaatgaat tgctgagtga attgatgcca aatattcctg aagaaattaa
    13501 agttataaaa aaatccttga ttttaaatta atagctacta tttccctttc agcaattcct
    13561 ccagctttat catttcatcg cggtactggg cagcttccag gaaatcgagt tttttggctg
    13621 cttcctgcat ggatcggcgg gcttttgcta tggctttttc gagtgccggt cgggacataa
    13681 actgtaccac gggatctgca gccagaatat tcggaatttc cggttcgatg taggcattcg
    13741 gttccatttg actgagtgaa tttcgttcgc ccttggttat ttgggtggga gttattccga
    13801 atttagcatt gtaggcaagc tgtttttcgc gccggcggtt ggtttcgtct atcgttttct
    13861 gcatgctcat agttatttta tcggcataca tgattacttt accattcaga ttgcgtgcgg
    13921 cgcgaccggc agtctgtgtg agcgaccggt gattgcgcag aaatccttcc ttatcggcat
    13981 cgaggatagc caccagcgaa acttcgggca aatccaaccc ttcgcgcaat aaattcaccc
    14041 ctaccagcac atcgtacacg ccccgccgga ggtcttccat tatctgcacc cgatcgagcg
    14101 tagttacatc ggaatgtatg tagttgcacc gcacattcat tctcaacaaa tactcagtca
    14161 attcttcggc cattcgtttg gtgagcgtgg ttaccagcac ccgttcgccg gcttcggttc
    14221 gcagggtgat ttcctccagc aggtcgtcaa tttgattgag gctgggacgc acatcaatca
    14281 ccggatcgag caaaccggta gggcgta
//
LOCUS       DAOPYB010000065        14293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4623_length_14293_cov_9.200660, whole genome shotgun sequence.
ACCESSION   DAOPYB010000065 DAOPYB010000000
VERSION     DAOPYB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14293
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4623_length_14293_cov_9.200660"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1370)
                     /locus_tag="PKH58_06695"
     CDS             complement(165..1370)
                     /locus_tag="PKH58_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88752.1"
                     /translation="MKNNYLLITLLALFATTTMSYADQTVKLRVQVPSQTVVVYATGG
                     FNGWSTSATPMTKVSDSPKTFEADITVPDNVDAGYNFKFLAGPDWAYEQGDPSADFTY
                     GAVGFTGAVVNSFKKYFTPMTPRDVKVNVLVPKTTLFCYITGGFANWEQPKKQMTFVS
                     EDNDGKVYTYTVPNIDPQLLEFKFASGPSWSYEQTQSSNFSYFGNGGANDEVSVVCSG
                     FKAIYDPVNVGDITVNITSTPAGTDSVYLVGSFGSGWSLAEAIAATKNNDGTFTAVIP
                     QVAIVEFKCWNRKDWAYEECQPDGSNLAANRQVTFKTTPVLNITVDRWKKMASGINEV
                     KYNFNYPVIVKNSRINVSGVLSAAKLYTINGQLMQTFKGSGEFTSKTLTKGIYILQVD
                     NARTKVLVD"
     gene            complement(1542..3032)
                     /locus_tag="PKH58_06700"
     CDS             complement(1542..3032)
                     /locus_tag="PKH58_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HNX88753.1"
                     /translation="MKKLGIYLYLATFVFIFASCSACRDNNTPQPPGTNDTIVKEVNT
                     YITTADKTMLFSSVAKSFSKGINMNIEKTLTLKPEQTFQTIDGFGAAITGSTCYNLLK
                     MTKADRTKLLEETFSVEKGMGYSYVRVAIGCSDFSLDEYSCCDVPGIENFAMPPYDVR
                     DLYPILKEILAINPNLKIMGSPWTPPRWMKVNNLTDLQPYNSWTSGQLNPKYYQDYAT
                     YFVKWIQAMEKEGFPIESITIQNEPLNRGNSASLYMTWQEQRDFIKTALGPKFAEAKI
                     KTKIVVFDHNFNYDNIPDQQGYPTRIYEDPDAAKYIDGAAYHAYGGSSSELTKIHNAN
                     PAKNLYFTEMSIGTWNYTFDGDLMWTMKEIGIGCLNRYCKAVIVWNLMLDDKRGPNRP
                     GGCTTCFGAVDVSSADYKTLDRKSHYYFIGHMSKALHSGSTRIGTSGYMPSGVNATAA
                     VNPNGTYGIVLQNENSTSVQMTIDDGNHHFDVTLPAKSITSCIWKK"
     gene            complement(3043..4446)
                     /locus_tag="PKH58_06705"
     CDS             complement(3043..4446)
                     /locus_tag="PKH58_06705"
                     /inference="COORDINATES: protein motif:HMM:NF028474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5121 domain-containing protein"
                     /protein_id="HNX88754.1"
                     /translation="MNKLHYIICSALLLLTASCQETEFRTHYPESTPTLNASVSENSI
                     QYGDSITLVADITDANPLSTLEVKVAVSNKLIISESIRTKGTSASIRKRYHIPFGTGM
                     PDKEKVKVYLTAINVEGFKTEQVLDNTIGNRPPMPKLYLIPASITSGLTTKELVLTDE
                     SNLIYSVSGIEFPNTFTCYVATKKTAFGRVDWTGLVFGMKGSEITLIKQGENPITVQD
                     ESLVNITSCSFDALNFKLNVAGKLLEPITNLDVMTDLAASPSSLLGADASKFRGATIY
                     FGKDVEVAFTGIDNVSNGLSPDYFEVTGTNKAKFLGETGMYKAYYHVDGKYLYIEPMP
                     TAAYPDALWICGVGLGRPSQPYATTTSWNWNTPLDYVPCRKISNGVYQATVYLENTDN
                     TEGTGYGTCNFKFFHYRGWDHGEESSMNYTVQTPLRNSNETGNVGNWRGNDATFSGVY
                     RITLDMNAKTTNLVKLN"
     gene            complement(4485..5996)
                     /locus_tag="PKH58_06710"
     CDS             complement(4485..5996)
                     /locus_tag="PKH58_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX88755.1"
                     /translation="MNKIIKTGLGLFILLLTACNLDYDPVSQLSERTLGTKDTTGTEI
                     KYKDREAMQSVYKSMYQRMTNQQEHWYLDYMLFAECRSDNAYAGTTGAEVVPVESNNL
                     DASNSVIARDWNRYLEDIALSNSIINNIDLVPDPALTAAERNRWKAEAKIFRAMMMYD
                     MVRWFGNFPVITKEAGKITAENIKEVYPLYFPKANTPAEAYAQIISDLRGSLDGAPAN
                     NNADKTILSKTVARALLAKVYADKQVQNPDSVIYFADQVFADGISLVNNYSDLFGLNT
                     AGTDAKVRNSSESILEMQYFTGSGNWVTWMFGRDLLDFDFYFTWAKWVTPSRDLIKAF
                     DDAGDMKRKNESIVYYQTSWSNYYDSKNYPFMYKCRSNVNSIIKLRGADILLLKAEAL
                     ARKNQLSQAAQVVNTVRKRVNLPDLTATNTSSKENLLDAILLERRLELAFEGQRWFDL
                     IRFDKLESIMNTINQRDSGRLPQGRAFTKDSELLPIPQPVLDENTNLVQNPGY"
     gene            complement(6027..9011)
                     /locus_tag="PKH58_06715"
     CDS             complement(6027..9011)
                     /locus_tag="PKH58_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX88756.1"
                     /translation="MRTKRKNFISMMVVLIMYLSCSITAWGQTTGNYRGTITDANREA
                     LIGVSVKVKGTTTGVVTNINGQYSIQARPGDVLVFSYVGMVTQEKILTSQLDNDIVLT
                     EEAKGLEEVVVIGYGVAKRKDVTTAVSSVSTEDLNLRPITSAAQAIQGKAAGVQVIQP
                     NGKPGAGMVIRVRGTTSMNASNDPLYVVDGVPMNNIDFLSANDIESMQIMKDASSAAI
                     YGSRGANGVVMITTKATARKDRSAVSFSAYTGISNVSRKMESLNLEEYREYLSDLKSS
                     VVLPDGLTDQTNWFDETFSTGIRHNYQLSASGASNKVNYFLSGGYTDETGIISSTSFK
                     RFNFRTSVESELYKWLNLSANISYSNNISKGDIISGTGSNRGGVILSVINTPTYAPVW
                     DTENPKYYYNKFYGVSNITHPLENLARTRDNKNDTHRLLATGKGVITFFPDLKLTSSI
                     TGDMTYSNYTSFLDPVSTSYGRGQYGEAQDNRSFNRVMMLDNILTYTKKIKSHNFEAM
                     AGTSYTVSDWSQSYQFASHFANDIVKTLNAANKISPGNGSTASQWAIMSYLGRLSYNY
                     ESKYLLSMNIRADGSSKLAPSGRWGYFPSVSAAWRISSEEFMSDLTWLNDLKIRAGWG
                     QTGNQDGLGDYAYLERYDFNRIEWWKPGQADAVPTYSQVSLSSQDLTWETTTQSNIGF
                     DAMLLHNRLTINADYYFKRTRNMLMWVTLPAGSAAVSSIQRNEGEMTNQGIELSLSSK
                     NLVGKFKWNTDFNISHNQNMLVKLDFKQVYYDARVTDILSDYAVKNMPGRPLGGFWGY
                     ESQGVDPETGELMYVDKNDDGFINASDRGYIGDPNPDFTYGMTNSFSFKNFNLSVLLQ
                     GTYGNDIFNVSRIESEGMYDAKNQSKRIVERWRRPGMITSIPKAGYDMKISSYFIEDG
                     SYLRVKDVTLSYDFSMNWMKKLGISKLQPYATVTNLITFTKYLGFDPEVNQWGNSGNV
                     QGIDYGTYPQTRSFIFGVNVDL"
     gene            complement(9291..10952)
                     /locus_tag="PKH58_06720"
     CDS             complement(9291..10952)
                     /locus_tag="PKH58_06720"
                     /inference="COORDINATES: protein motif:HMM:NF039744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6377 domain-containing protein"
                     /protein_id="HNX88757.1"
                     /translation="MSNRKYIFICIISLISLSVSATDIETDSLIRIYDKLIETGNIHI
                     RERQQRIDNLVNKLNRQQHSPNNKYILYQKIYEEYRPYISDSAIAYLNRCITTAEQLG
                     DSNLVTDSKLKLSYLLASIGLYKESADILATIREADLPQSQLIEYYFCMEHAYGEMSY
                     YSKEARLSNSYREISNKYKELQKKILPHNDDRYLSIIESDYRNIRDFKMSLKINDIRL
                     KSFSENSREYALIAFLRSLTYKESGDELLQQKWLLKSAISDLRFGITDNASSWELANL
                     MYKRGDIKHAHLYINYSVNNATIFNARLRFTQIAEVQSIINNAYLIDKAQQEKKLRRS
                     LILISILTLLLLTSVIGIFFQNRRISKANAKTIKANDELNRLNWELSRINSEINNTNA
                     ELSESNQVKEEYIGHFLSLCSLYIDKIDAYRKTIRKKLLNNQQSEVMEDVKSSDFMDN
                     ELKEFYNNFDRSFLRLYPDFVHEFNNLLMDEEKIILKKDELLNTELRIFALIRLGIDN
                     SAKIAELLHYSANTIYNYRAKIKNKSKVSRNDFENMVKKIGTFTR"
     gene            11557..12840
                     /gene="uraA"
                     /locus_tag="PKH58_06725"
     CDS             11557..12840
                     /gene="uraA"
                     /locus_tag="PKH58_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HNX88758.1"
                     /translation="MSRRTIGIHEKLPVLESIPLSFQHLAAMFGATILVPILVGVNPA
                     IALLMNGIGTLIYSWVTKGGIPAYLGSSFAFIAPIILISTSYGGFSHAQSGFIFFGLF
                     FILISFVVKKWGVGWIDVAMPPAVMGAVVAIIGLELAPVAAQQAGLAAGSTPVGQVVQ
                     PFVMNNDVVMVSMFTLAVGIFGSVLFRGFLQVIPILIAVIAGYIFALAKGMVDTQVIT
                     DASWFALPHFQAPVFDLNAVLIIMPACIVVLAEHISHLIVTGKVTETDLIKEPGLHRS
                     LLGDGISNLVSGFVGSPPNTTYGENIGVMAITRVFSVWVIRGAALLAIAFSCIGKVSA
                     AISTIPGAVMGGITFLLYGVIAVQGFRMFVEQKVDFSKNSNMVLGAVTFVVGVSGASI
                     KIGSVELKGMAFAAVTGVALSLIFWGLKKLKWMEE"
     gene            complement(13130..>14293)
                     /locus_tag="PKH58_06730"
     CDS             complement(13130..>14293)
                     /locus_tag="PKH58_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX88759.1"
                     /translation="PAYVQIEDDTTPLEVGRGYLAQLKSNNTYLYSGTLNNGTISLTP
                     TRTGTKASARGFNLVGNPYPSYLNWKSAEITKTNIRQTIWFRTLSKNSANGTMVFDTY
                     DGTVGTGNGETGVVSQYIPPMQGFWVKVDTDNKAASLIFRNAARLHQDVPANVIKTPS
                     EENDGIQILRLKVNTPATGDETIIVGNSSATDDYDSFDSDKMSNTDPSVPEIFTLASG
                     HELVINHLNRISADKTLALGFRPGTAGTFAIEATQFDNINNRVMLLDRLKNIEQELTA
                     GTSYSFTSDSVATNDRFVIRFVPGSANGLDISQDNKTLNVYCTSDNRIQIIYHGELNE
                     QTSVTVYNPAGQKLSTTKLNRETTQLSETYQPGVYLVQLTDDGKKITKKLIIK"
BASE COUNT         3681 a         2960 c         3129 g         4523 t
ORIGIN      
        1 tcggtcggtt gtttgcagat aatcagtcat taggccttta tgcggataat cataaagttt
       61 ataaggaaat ccggacaatc aaacaaatcg ttgttcatca gactgaaaga gaccgttaac
      121 acacaggtta acggtctcta ctaaagcatc actaatctgt ctaatcaatc caccagtact
      181 ttggtacggg cattgtccac ctgaaggatg tatatgcctt tggtaagagt tttgctggta
      241 aattcaccgc tgcctttgaa agtctgcatc aactgaccat tgatggtgta cagtttagca
      301 gccgaaagaa caccgctaac gtttatccgg ctgtttttca ctatcacagg ataattgaag
      361 ttgtacttaa cttcattaat gcccgaagcc atttttttcc agcgatcgac agtaatgttg
      421 agtacaggag tggttttgaa ggtaacctga cgatttgccg caaggttcga tccatcgggc
      481 tgacattctt cataagccca gtctttgcgg ttccagcatt tgaattctac gatagctacc
      541 tgaggaatta ccgctgtgaa tgttccgtcg ttgttttttg tggcggcaat tgcttcagcc
      601 aggctccatc cgctaccgaa actgccaacg agatagacgc tgtcggtacc tgccggagtt
      661 gaagtgatat tgactgtaat atcgcctaca ttaacgggat cgtagatagc tttgaaaccg
      721 gaacatacca cgctaacttc atcgttggca ccaccatttc caaaatatga gaagttgctt
      781 gattgcgttt gttcatagga ccaactcgga ccggaagcaa acttgaactc cagcagttgc
      841 gggtcaatat tcggaactgt ataggtataa acttttccgt cattatcctc acttacaaag
      901 gtcatttgtt tctttggctg ttcccagttg gcaaaacctc cggtaatgta gcagaagagt
      961 gtggttttag gaacaagaac gtttacctta acgtcacgtg gtgtcatagg agtaaaatac
     1021 ttcttaaatg aattaaccac tgcaccggta aatccaactg caccataagt aaaatcggct
     1081 gaaggatcac cttgttcgta agcccaatcc ggtccggcaa ggaatttgaa gttgtaacct
     1141 gcatccacat tatcgggtac cgtaatgtct gcttcgaatg tttttggtga atcggaaact
     1201 ttggtcatgg gggtggctga agtactccac ccattaaaac cacctgtggc gtaaactact
     1261 acagtttggg atggaacctg tactctgagt tttactgttt gatcagcgta actcatggtt
     1321 gttgttgcaa aaagtgcaag caacgtaatt aaaagatagt tgtttttcat aagacatttg
     1381 tttttagaga ttgtttttaa tgtttgtaaa ttgtaatacc tctatttgag ttaagatagt
     1441 tcatcatttc agtaatgtct gttctttccc cccgctttcc atgatacgga gagcggggag
     1501 ggaacagatt gttctgatta atcatgttat cgtgcctaaa atcacttctt ccagatacat
     1561 gatgttatac tctttgcagg caaagtcaca tcaaaatgat gattaccatc gtcaatggtc
     1621 atctgaaccg aagttgaatt ttcattttgc agtacgatgc cgtaagttcc gttgggatta
     1681 acggcggcag tagcattcac acctgatggc atgtagcccg aagtgccgat acgtgttgaa
     1741 ccgctgtgca gcgcttttga catgtgaccg ataaaataat aatgagattt tctgtcgagt
     1801 gtcttgtaat cagccgaact aacatccaca gcgccaaagc aggtggtaca tccgccgggt
     1861 cggttaggtc cccgcttgtc gtcgagcatt aagttccata caattacagc tttgcaatag
     1921 cggttcaggc atccgatgcc tatttccttc attgtccaca tcaggtcgcc gtcaaaagta
     1981 taattccagg tgccgattga catttcggta aaatacaggt tcttggcagg gttggcattg
     2041 tgaatttttg taagttcact gctgctgcca ccgtaggcat gataggcagc tccgtcgatg
     2101 tatttcgccg cgtcgggatc ttcgtaaatg cgtgtgggat aaccctgttg atccggaata
     2161 ttgtcgtagt tgaaattgtg atcaaacacc acaattttcg tttttatttt ggcttccgca
     2221 aacttcggtc ccagtgctgt tttgatgaaa tcgcgctgtt cttgccaggt catgtataac
     2281 gaagcggaat ttccacggtt gagcggttca ttctgaatgg ttattgactc gatggggaaa
     2341 ccttcctttt ccatagcctg aatccatttt acaaaatatg tcgcgtaatc ctgataatac
     2401 ttcggattga gttgtccgct cgtccaggaa ttatacggtt gcagatcagt caggttgtta
     2461 actttcatcc atcgcggcgg agtccacggc gaacccatta tcttcagatt gggattgatg
     2521 gccagaattt ctttcaggat tggataaaga tcgcgtacat catagggagg catcgcgaag
     2581 ttttctattc cgggtacatc acaacaactg tattcatcga gtgagaaatc agagcatccg
     2641 attgccacac gcacataact gtatcccatt cctttctcta cactgaatgt ttcttccagc
     2701 agttttgtcc ggtctgcctt cgtcatcttc agcaggttgt aacaggtaga gccggtgatg
     2761 gcagcgccaa aaccatcaat ggtctgaaaa gtctgttcag gtttcagtgt cagggttttt
     2821 tcaatattca tattgatacc cttactgaaa ctttttgcca cagagctaaa cagcatggtc
     2881 ttatcggcag ttgtaatgta tgtgttaacc tctttgacaa tggtgtcgtt cgttcccgga
     2941 ggttgtggtg tgttgttatc cctacatgcg gagcagcttg caaagatgaa aacaaaagtg
     3001 gctaaatata aatatattcc gagtttcttc atgactgttg tgttaattca atttaaccaa
     3061 attggttgtt ttggcattca tatccagtgt tatcctgtac acaccgctga atgtggcgtc
     3121 atttcccctc cagtttccaa cattacctgt ttcgttgctg tttctgagtg gtgtctgtac
     3181 cgtgtagttc atcgatgatt cttcaccgtg atcccagccc cggtaatgga agaatttgaa
     3241 gttacatgtt ccgtaaccgg ttccttccgt attgtccgtg ttctcgagat aaacagttgc
     3301 ctgataaact ccgtttgata ttttccggca gggtacataa tccagcggtg tattccagtt
     3361 ccagctggta gtggtagcgt aaggctgcga gggccggcca agtcccacac cgcaaatcca
     3421 taaagcatcg ggatatgctg ctgttggcat cggttcgata tacaaatatt taccatctac
     3481 gtgataataa gctttgtaca tacccgtttc acccaggaat ttcgctttgt ttgttcccgt
     3541 tacttcaaag tagtcaggcg acaatccgtt ggagacgttg tcaattcctg taaatgctac
     3601 ttcaacgtct ttgccgaaat atattgtagc tcccctgaac tttgaggcat cagcgccaag
     3661 cagcgacgaa ggtgaagctg ccaggtcggt cattacatcc aggtttgtga tgggttcaag
     3721 cagtttgccc gctacattca atttgaaatt caacgcgtcg aaactgcatg aggtgatatt
     3781 aacgagcgat tcatcctgaa cggtaatggg attttcaccc tgtttgatca gggttatttc
     3841 acttcctttc attccgaaaa ccagtcctgt ccagtcaact ctgccaaaag ccgttttttt
     3901 tgtagccaca tagcaagtga acgtattggg gaattcgata ccgctaactg aatagatgag
     3961 gttactttcg tcggtcagga ctaattcttt ggtagtaagt ccgctggtaa tgcttgcagg
     4021 tattaaataa agctttggca ttggaggtcg gttgccgatg gtgttgtcaa gcacctgttc
     4081 tgttttaaag ccctccacat tgatggcggt aagatatacc ttcacctttt ccttatcggg
     4141 catacctgtt ccgaacggga tatgatatct tttccggatg cttgccgatg tgccttttgt
     4201 ccgtatggat tcggaaatga tcagcttgtt actgactgcc actttaactt cgagtgtgga
     4261 gagcggatta gcgtcagtaa tatctgccac caacgtgata gaatctccat actgaatgga
     4321 attctccgat acacttgcat tgagtgttgg agtgctttcg gggtaatgcg ttctgaactc
     4381 tgtttcctgg catgaagctg taagcaggag aagtgcggaa cagatgatat aatgtagttt
     4441 attcattttt tacaatttta tcggttagca aaaacgtctg ataattaata acccggattt
     4501 tgaaccagat ttgtattttc atccagtacg ggttgtggta tcgggagcag ttcggagtct
     4561 tttgtgaatg cccttccctg tggcaatctt cccgaatcgc gctgattgat ggtattcatg
     4621 atactttcca gtttatcaaa ccgtatcaaa tcaaaccatc tttgaccttc gaaagccagt
     4681 tccagtcttc tttcaagcaa aatcgcatcc agcaggtttt cctttgatga agtattcgtg
     4741 gcagttaaat cgggcaggtt tacccttttt ctaactgtat ttacaacctg cgctgcctgt
     4801 gatagctgat ttttgcgtgc cagggcttcg gctttaagca acagaatatc cgctccgcgt
     4861 aatttgataa tggaattgac atttgaccgg catttgtaca tgaacggata attcttggaa
     4921 tcgtaatagt tggaccacga agtctgatag tacacgatag attcattctt acgtttcatg
     4981 tccccggcat catcaaatgc cttgatcagg tctcttgatg gtgtaaccca tttagcccag
     5041 gtaaaataga aatcgaaatc cagcaggtca cggccaaaca tccatgttac ccagttacca
     5101 cttccggtaa aatactgcat ttctagaatg gattcagagg agttgcggac tttggcatcg
     5161 gttccggcag tgtttagccc gaacaaatcg ctgtaattgt ttaccagact aatgccatct
     5221 gcaaaaacct gatcggcgaa ataaatgaca ctatccggat tttgaacctg tttatcggca
     5281 tataccttgg ccagtaaagc acgggcaacc gttttggaaa gtattgtctt atccgcgttg
     5341 ttgtttgccg gagcaccatc gagtgaacca cgcaggtcgc tgatgatttg cgcgtaggct
     5401 tctgccggtg tatttgcctt cgggaaatag aggggataga cttccttgat attttcggca
     5461 gtaattttac cagcttcttt cgttataacg gggaaattac caaaccagcg caccatatca
     5521 tacatcatca tcgctctgaa gatctttgct tcggctttcc atctgttacg ttcagcggca
     5581 gtcaaagcgg gatcgggtac cagatcgata ttgttgataa tgctgttgga aagggcaata
     5641 tcttcgaggt aacggttcca gtcacgggca attacgctgt tgctggcatc gaggttgttc
     5701 gattccacag gcaccacttc agctccggtt gttccggcat aagcattgtc cgaccgacat
     5761 tccgcgaaaa gcatatagtc gagataccag tgttcctgct gatttgtcat gcgttggtac
     5821 atacttttat ataccgattg catggcttca cgatccttgt attttatttc ggtgccggta
     5881 gtatcttttg ttccgagtgt acgttcggag agttgtgaaa cgggatcata atccaggttg
     5941 catgctgtaa gcaacaggat aaataatcca agtccggttt ttattatttt attcatgatt
     6001 ttcgatgttt aaagatttga taaatattaa agatcaacat taaccccaaa gataaaggag
     6061 cgtgtttgcg gataagttcc gtaatctatt ccctgtacgt tgccagaatt tccccattga
     6121 ttgacttcag ggtcaaagcc gaggtacttg gtgaatgtga tcagatttgt caccgttgcg
     6181 tatggctgaa gtttacttat tcccagcttt ttcatccagt tcatcgagaa atcatacgac
     6241 agggttacat ccttgacgcg cagataactt ccatcttcga tgaagtagga cgatatcttc
     6301 atatcataac cagctttggg tatcgaagta atcatgccgg gcctgcgcca gcgttcaaca
     6361 attcttttgg attgattttt agcgtcatac ataccttcgg attctattct tgatacgttg
     6421 aatatatcat tgccgtatgt tccctgtaat aacacactta aattaaagtt tttgaaagaa
     6481 aagctatttg tcatcccgta tgtaaaatca ggattaggat caccgatata gccacggtcg
     6541 gacgcgttga taaatccatc atcgttttta tcgacataca tcagctcgcc agtttcgggg
     6601 tcaacaccct gggattcata tccccaaaaa ccacccagcg gtcttcccgg catatttttc
     6661 actgcataat cggaaagaat atccgtaaca cgggcatcgt aatacacctg tttgaagtcc
     6721 agttttacca gcatgttttg gttgtgtgat atattgaaat cggtattcca tttgaatttg
     6781 ccgaccaggt tcttggaact aaggcttaat tcaataccct gattggtcat ttcgccttcg
     6841 ttgcgctgaa tggaagagac agcggctgaa cctgcgggca gtgtcaccca cataagcatg
     6901 ttgcgtgtgc gtttgaaata ataatcggcg ttgatggtca gcctgttatg caacaacatg
     6961 gcatcaaatc ctatgttact ttgcgtggta gtttcccatg tcaggtcctg tgaacttaaa
     7021 cttacctgag agtatgtggg taccgcgtca gcctgccccg gtttccacca ttctatccta
     7081 ttgaaatcat atctttcaag gtatgcatag tcgcccagac catcctgatt acctgtttgt
     7141 ccccatccgg cacgaatttt caaatcgttg agccaggtca aatccgacat gaactcctcc
     7201 gaggatatgc gccatgctgc cgataccgac gggaagtagc cccaacgacc ggaaggcgcg
     7261 agtttgcttg aaccatcggc acgtatattc atactgagca ggtatttgct ttcataatta
     7321 tatgataatc gccccagata tgacatgatt gcccattgtg aagcggtact accgttaccc
     7381 ggactgattt tatttgctgc attcagtgtt tttacaatgt cgttggcaaa atgtgaagcg
     7441 aactgataac tttgcgacca gtcagatacc gtgtatgagg tacctgccat cgcttcaaaa
     7501 ttgtgcgatt tgattttctt tgtgtaagtg agaatattat caagcatcat cacacggttg
     7561 aatgaacggt tgtcctgagc ttcgccgtat tgtcctcttc cataactggt agaaacggga
     7621 tcgaggaatg aagtgtagtt ggaataggtc atatcgcccg tgatggagga tgtaagtttc
     7681 aaatccggga agaaggtgat aacacctttt cctgttgcca gcaagcggtg cgtgtcattc
     7741 ttgttgtcgc gggtgcgggc cagattttcg agcgggtgag tgatattgct tacaccgtag
     7801 aatttgttgt aatagtattt aggattttca gtatcccaca caggcgcgta ggtcggagta
     7861 ttgatgaccg ataaaataac accacccctg tttgatccgg ttcctgagat aatgtcgcct
     7921 ttggatatgt tattggaata agaaatgttc gcgctcagat taagccactt gtacaactcg
     7981 gattcgaccg atgtgcggaa attgaagcgt ttaaaagatg ttgaactgat aataccggtt
     8041 tcgtcggtat aacctcctga aagaaaatag ttgactttat tcgaagctcc ggatgccgat
     8101 agctgataat tatgccgtat tccggttgaa aatgtttcgt cgaaccaatt ggtttgatcc
     8161 gttaatccat cgggtaaaac aaccgatgat ttaagatcgg acagatactc cctgtattct
     8221 tcaaggttaa gcgattccat tttgcggctg acattggata tacccgtata agcactgaat
     8281 gaaacagcag aacgatcctt gcgcgctgtg gctttggtag tgatcataac cacgccattg
     8341 gcgccacgcg aaccatagat ggcagccgat gaggcatcct tcatgatctg catactttca
     8401 atatcgttgg cagaaaggaa atcgatattg ttcatcggaa ctccgtctac tacgtaaagc
     8461 gggtcattgc tggcgttcat ggaagtggtt cccctcacgc ggatgaccat accggcaccc
     8521 ggcttaccat tgggctggat aacctgcaca ccggcagctt taccctgaat agcctgagcg
     8581 gctgatgtta tcggtctcag attcagatct tcggttgaaa cagaggaaac tgccgtggtc
     8641 acatcttttc ttttggctac accataacca attaccacca cttcttccag ccccttggct
     8701 tcttcagtca gaacaatgtc attatctaac tgagaagtca ggattttttc ctgtgtcacc
     8761 attccaacat acgaaaatac aagtacatct cccggtcggg cctgaatgct gtactgtccg
     8821 ttaatgttgg taaccactcc ggtggtagtt ccttttacct ttacacttac accaatcagc
     8881 gcttcgcggt tggcatcagt gattgtgccc cgatagttgc cggtagtctg gccccacgct
     8941 gttattgaac agctgagata cattatcagc acaaccatca tggaaataaa attttttctt
     9001 tttgttctca tgtgtatttg gattttttgt ttgaatatat attgttaaca ttacagattt
     9061 aagcattaat gattgcccga ctataaaagt cgggttgcag gcataatatt gaaacaattt
     9121 gcttaattta aggtttaagc aaatgtacaa tagaatatca gaaaatattt aagaaataaa
     9181 tatttggggg atggttgtag gaatataaac gctgatattc agtaatataa caaacttgta
     9241 ggcgcgggaa taagtggatt tttcaggaat aaaattatat cgtattgtat ttatctggta
     9301 aatgtaccaa tctttttaac catattctca aaatcattac gggatacttt tgacttattc
     9361 ttaattttag cgcggtaatt gtaaatggta ttagcagagt aatgcaataa ttcggcaatt
     9421 ttggcactgt tgtcgatacc cagacgtatt aatgcaaaaa tacgtaactc ggtatttaat
     9481 aattcatcct ttttgaggat aattttttcc tcgtccatca gcagattatt aaattcatga
     9541 acaaaatcgg gatagagacg cagaaaagaa cggtcgaagt tgttgtaaaa ctctttcaac
     9601 tcgttgtcca tgaaatctga tgactttacg tcttccatca cttcggattg ctgattgttg
     9661 aggagtttct ttcgaattgt ctttcggtag gcatctatct tgtcgatgta caacgagcat
     9721 agcgatagaa aatgtccgat atactcttct tttacctgat tggattccga cagttcggca
     9781 ttcgtattat ttatttcact gtttattctg cttagttccc aattcagccg gttcagttca
     9841 tcattcgcct ttatggtttt cgcgttggct tttgatatcc tgcggttttg gaaaaaaatt
     9901 ccgataacag atgtcagtaa cagcaaggta agtatactga taagaataag tgatctgcgt
     9961 aattttttct cctgctgagc tttgtcaatc agataggcgt tatttataat cgactgtact
    10021 tcggctattt gtgtaaatct cagacgcgcg ttgaaaattg tcgcgttgtt gacggaataa
    10081 ttgatgtaca ggtgagcatg ttttatatct ccccgcttgt acatcaggtt ggccagctcc
    10141 cacgaggagg cattgtctgt aattccaaat ctgagatcgc tgatagccga cttcagcagc
    10201 catttttgtt gcaacagctc atcgccgctc tccttataag tcaatgatct cagaaatgca
    10261 atcaaggcat attcccggct attttcggaa aatgacttca gtctaatgtc atttattttc
    10321 agtgacatct tgaagtcgcg tatattcctg taatcggact cgatgatact cagatagcgg
    10381 tcgtcattgt gaggtaagat ttttttctga agctctttat acttattgga tatttcgcga
    10441 taactgttgc ttagccgtgc ttctttactg taatatgaca tttcaccata tgcgtgctcc
    10501 atacaaaagt aatattctat cagttgtgat tgtggcaagt cagcttcgcg aatagtggca
    10561 agtatatcgg cagactcctt gtaaagtccg attgacgcga gaagatatga aagttttagc
    10621 tttgaatcgg ttacgaggtt ggagtctccc agttgctccg cggttgttat gcatcggttg
    10681 aggtaggcaa ttgccgagtc cgatatgtaa gggcggtatt cttcgtatat tttttgatac
    10741 aggatgtatt tattattggg tgaatgttgc tgacggttta acttattaac cagattatct
    10801 atccgttgct gtctttcacg tatatgtata tttccggttt ctatcagctt gtcgtatatg
    10861 cgaatgagag aatctgtttc aatatctgta gctgagaccg ataatgagat taaacttata
    10921 atgcagataa aaatatactt tctgttactc atatttgaag tttcaataat agggtcaaag
    10981 tacttagcac cggagaaatg aaataaagca gtccgcaatt cctttattta taatatggtc
    11041 tataacgtct gcttcagtac aaaattatta aaaagatccg gaataataac tccaaaaatc
    11101 aatcaaaccg cgaaatgttt aataaattaa atgacataaa gctggaaatg gggcttttct
    11161 ttcagacgtt tttgaaggat gtcattatcc cgtttcagct ttccttttga tgtttgtaaa
    11221 aaaaaaagtt taataaaaac aggtttagga tcgaaagatt tctcagtagt aacttcaaag
    11281 taaaaaaaac ctaaatattt ataaacagaa gccctacgga atttcatagt tttttgtaat
    11341 tttgacagat ttttctggta tccacaaatt tcccgtttca aaattgattg agttgttcga
    11401 agtgtgattt ataatctttc cccaaatcgc tgtatttgaa aagattgcaa attatacgcg
    11461 attgctctgt acgtgcctga atgaatagag aaatcgttgc agaaatgtat tttttatttt
    11521 caaatcaaaa tatattacat aaaaactaaa aaaactatga gcagacgaac aatcggcatt
    11581 catgagaaat tgcccgtact tgaaagtatt ccgttaagtt ttcagcacct tgctgccatg
    11641 tttggcgcca ccattctggt accgatactt gttggtgtta atccggctat tgccttgctg
    11701 atgaacggta tcggcaccct gatttattcg tgggtaacca aaggaggaat tccggcttac
    11761 ctgggttcca gttttgcatt tattgccccg attatcctta tcagcacaag ttatggaggt
    11821 ttcagccatg cccaatccgg atttattttc ttcggattat ttttcatcct gatttccttt
    11881 gttgtaaaaa aatggggtgt gggctggatt gacgtagcca tgccacccgc cgtgatgggt
    11941 gccgttgtgg caattatcgg actggaactt gctccggttg cagctcagca ggccggtctt
    12001 gctgccggtt ctactccggt cggacaggta gtgcagcctt ttgttatgaa caacgatgtg
    12061 gtcatggttt cgatgttcac actggcagta ggtatattcg gatcagtact tttcagaggt
    12121 tttcttcagg ttattcccat tctgattgcc gttatagccg gttatatttt cgcccttgcc
    12181 aaaggaatgg tcgatacaca ggtaataact gatgcatcgt ggtttgccct gcctcatttt
    12241 caggcacccg tttttgattt gaatgctgtt ctcataatca tgccagcctg tattgttgta
    12301 cttgccgagc atatcagcca tttgatcgtt acgggtaaag ttaccgaaac cgacctgatc
    12361 aaggaacccg gacttcaccg ttcactactg ggtgacggta tttccaacct cgtttcgggt
    12421 tttgtaggtt cgccccccaa tactacttat ggtgaaaaca ttggtgtgat ggctattacc
    12481 cgggtatttt ccgtatgggt aatccgcggt gctgctctgc tggccatcgc attttcctgt
    12541 atcggaaaag tttcagcggc aatttccact attccgggag ctgtaatggg tggtatcacc
    12601 ttcctgcttt acggtgtgat tgctgttcag ggtttccgta tgtttgtgga gcagaaagtt
    12661 gactttagca aaaacagcaa tatggtactt ggagccgtta ctttcgttgt gggtgtgagc
    12721 ggtgcaagca ttaagattgg tagtgtggaa cttaaaggga tggcatttgc tgctgttaca
    12781 ggagttgcac tttctctgat attctgggga cttaagaaac tgaaatggat ggaagagtaa
    12841 tttgttaatt tgaagatgta ccaatttgaa aatttgagaa ttggttattg agttactaag
    12901 ttatttataa tttataattt ataatttata atttataatt cataattcat tttcctcttc
    12961 cggatacaac aaaaaaacag actttgttca caacgaacaa ggtctgtttt tttatttggc
    13021 tcttgaagaa taatactatt cggaacaaaa tcactttctt tagctttatg tctttaattt
    13081 tcagcctagt atcttatatc catttgtctg gtatctattt gtcttacacc tatttaataa
    13141 tcagtttctt tgttattttc ttcccgtcgt cagtcagttg aaccagatac acacccggct
    13201 ggtatgtttc actgagctgg gtagtttcac ggtttagttt ggtggtactc agcttttgac
    13261 cggcagggtt gtacactgta acagatgttt gttcgttgag ttctccgtga taaataattt
    13321 gtatccggtt atccgaagtg cagtacacat ttaaagtctt attatcctga gaaatgtcca
    13381 atccattggc tgagccaggt acaaatctga tgacaaatct gtcgttggta gcgacgctgt
    13441 ccgaggtaaa actgtacgat gtaccggcag tgagttcctg ttcgatattc ttcagtctgt
    13501 cgagcagcat cacgcggtta ttaatattat cgaactgagt agcttcgatg gcgaatgttc
    13561 ctgctgtacc cggacgaaaa ccaagcgcta atgttttatc agcactaatt ctgttcaagt
    13621 ggttaattac cagttcgtga ccggaagcca gtgtaaatat ttcaggaact gacgggtcgg
    13681 tgtttgacat tttatcagaa tcgaaactat cgtaatcgtc agtagctgag ctatttccaa
    13741 cgataatcgt ttcgtctccg gtggcaggtg tatttacctt caatcgcaga atctgaattc
    13801 cgtcattctc ctccgaagga gttttaatta cgttagccgg tacatcctga tgcaagcggg
    13861 ctgcatttct gaaaatgagc gacgccgctt tgttgtccgt atccaccttc acccagaaac
    13921 cctgcatggg cggaatgtac tgcgatacaa cacccgtttc gccatttccg gttcctaccg
    13981 taccatcgta cgtatcaaaa accatcgttc cgttggctga atttttggat aaagtacgaa
    14041 accagattgt ttgtcgtata ttcgttttag taatttcggc agatttccag ttgagatagg
    14101 atggatacgg atttcccaca aggttgaatc cacgtgctga agctttggtt cccgtacggg
    14161 tgggtgtcag gctgatagta ccgttgttga gcgtgccgga gtagaggtaa gtgttgttgc
    14221 ttttcagttg agccaggtat cctctgccca cttcaagggg tgtagtgtca tcttcaatct
    14281 gaacatacgc agg
//
LOCUS       DAOPYB010000066        14119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4701_length_14119_cov_9.685509, whole genome shotgun sequence.
ACCESSION   DAOPYB010000066 DAOPYB010000000
VERSION     DAOPYB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4701_length_14119_cov_9.685509"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..2843)
                     /locus_tag="PKH58_06735"
     CDS             complement(102..2843)
                     /locus_tag="PKH58_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNX88760.1"
                     /translation="MAKDEELNGIEEQEIPDEETAESTLETDDLETDNKTHSSYKIPK
                     ISDDAVKHHLSGMYQNWFLDYASYVILERAVPDIYDGLKPVQRRILHSMKRLDDGRYN
                     KVANIVGHTMQFHPHGDASIGDALVQLGQKDLLIDCQGNWGNILTGDGAAAPRYIEAR
                     LSKFALETVFNPKITEWKPSYDGRNKEPIDLPVKFPLLLAQGVEGIAVGLNSKILPHN
                     FNELIDASVAYLRGEDFVLYPDFQTGGSIDVSRYNDGERGGAVKIRSKIGKIDNKTLV
                     ITEIPFGTDTSKLIESIIKASEKGKIKIRKVDDNTSANVEILIHLSPGTSSDKTIDAL
                     YAFTQCELSISPNCCVIHDKRPRFMGVSDLLRISTDHTKDLLEQELIIQRNELSEQLF
                     FVSLEKIFIENRIYKDKEFEESTNQDEVVAHIDKRLEPFKKDFIREVMREDILKLLEI
                     KMVRITKFDSDKANDTIIGIQKKIKEIEYNLDHLIDYTIFWFENLKDKYGKNYPRHTE
                     IRNFDTIVAAKVVEANEKLYVNHEEGFIGTALKKDEFVCNCSDIDDVIVFYKDGKYKV
                     VKVAEKLFVGKNILYINVFKKNDKRTIYNAVYRHGKTGPYYMKRFAVTGITRDKEYDV
                     TQGKDGSKIIYFTANPNAEAEVIRVTLKPKPRLIKLVFEKDFSDIAIKGRQSMGNMLT
                     KNDVHRIALKQKGGSTLGGREVWFDRDILRFNYDNRGEYLGEFLSDEQVLVISTKGEY
                     RTTNFDLSNHFEKDILIIEKFDPNKVWSVVLYDADQKFHYLKRFQLEPSAKPVSFLGE
                     NPASQLILMSGEDFPRFEVTFGGGDAFREALVVDAEEFIGVKSYKAKGKRLTTFEIES
                     INELDPLRFKTEDKPDEDNKPEENDEENETPENNEPIDPEDDNQKIIDDITGQMTLF"
     gene            3016..3618
                     /locus_tag="PKH58_06740"
     CDS             3016..3618
                     /locus_tag="PKH58_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88761.1"
                     /translation="MKQLLNLKLLILSAILFTFSATYAQTADKGKIGFTYSLNGGTNV
                     LQPALMGAASHTVTDASGIGLIYLRPINHWLELETGMNYSLYKITTTSAPTPQVTTSN
                     STLSLIDIPIGVRAGFWKYFFANGGLLLDLDLMSNSHVDSQNGIGLMLGVGVKYDFKF
                     GGSLFANPYGKIHSLLPFGFEKYHERILESGWKFGFTYKL"
     gene            3866..5413
                     /locus_tag="PKH58_06745"
     CDS             3866..5413
                     /locus_tag="PKH58_06745"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNX88762.1"
                     /translation="MASQEDVFKKLIAHCKEYGFVFPSSEIYDGLSAVYDYGQNGVEL
                     KNNLKKYWWDSMTLLHENVVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEDLLAKYDEKIEKEVEKAAKRFGESFDPVMFRQTNPRVLENQTKRDEIHARFVK
                     ALNDSDLDELKQIIVDYEVVCPISGTKNWTDVRQFNLMFSTEMGSTADGALKIYLRPE
                     TAQGIFVNYLNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GEEMKWFEFWKQFRLRWHKALGLGDTKYRFHDHDKLAHYANAATDIEFEMPFGFKEVE
                     GIHSRTDFDLGSHEKYSGKNIKYFDPELNQSYTPYVIETSIGVDRTFLSIMAAAYTEE
                     ELEGGDSRVVLKLPPVLAPIKAAILPLVKKDGLPEKAREIMNELKFDFKCAYDEKDSI
                     GKRYRRQDAIGTPFCITVDHQTLEDNTVTIRHRDDMTQERIAMSELNKIIGDYVNMKN
                     LFRKIVD"
     gene            complement(5516..5821)
                     /locus_tag="PKH58_06750"
     CDS             complement(5516..5821)
                     /locus_tag="PKH58_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNX88763.1"
                     /translation="MIKHIVCFQLADEAEGKTKAENAQIIKEGLENLVNLIPQAKKIE
                     VGINHPDTPVSNYDIVLYCEFDSIDDLNIYQEHPEHKKVGAYIGKVKTGRVCVDYEV"
     gene            complement(5834..7444)
                     /gene="nadB"
                     /locus_tag="PKH58_06755"
     CDS             complement(5834..7444)
                     /gene="nadB"
                     /locus_tag="PKH58_06755"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNX88764.1"
                     /translation="MARKYDFVVVGGGIAGISFALKAASYGKVALLCKTTLEESNTSY
                     AQGGIATVMYEPDNYKKHIEDTLIAGDGHCDITAVELVVKNARAQIDQLLEWGMDFDK
                     TEDGNQFDLHKEGGHSEFRILHHKDNTGFIIQESLNKQIRNHPGIDLYEHYFAIEILT
                     QHHLGEIVTHHTPDIECYGVYALNQKSNHIHTFLSKVTILATGGIGSVYQASTNPAIA
                     TGDGVAMVHRARGVIRDMEFVQFHPTALYHPGDKPVFLITEAIRGYGAVLRGRDGQEF
                     MQKYDSRGSLAPRDIVARAIDNEMKIHGDSFEYLDVTHKNAEETKEHFPMIYHKCLEY
                     GIDITKDYIPICPTQHYICGGIAVDIHGQSSVNRLYALGECSATGLHGANRLASNSLI
                     EAIVYAEEAVKHAAPRLDQLTFNEIIPDWNDEGTSLTEEMVLITQSFREVEQIMSTYV
                     GIVRSNLRLQRAMSRLEILFRETESLFNRSVVSREICELRNVISVSYLIIKQAIARKE
                     SRGLHYTVDYPKKMEEEKNAQEYTKKKK"
     gene            complement(7501..8910)
                     /gene="rlmD"
                     /locus_tag="PKH58_06760"
     CDS             complement(7501..8910)
                     /gene="rlmD"
                     /locus_tag="PKH58_06760"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNX88765.1"
                     /translation="MAKNKKQLPLLENVTITDIAAEGKAIAKVNDIVVFVPFAVPGDV
                     ADLQVTRKKSSFMEARVVDIVKPSEIRQEPFCEHFGICGGCKWQILPYAEQLKYKQNE
                     VENNLRRIGKVQLPEISPILGSAKTIFYRNKLEFTFSNRRWRTFEEMNSAKEFDTMNA
                     VGFHIPGMFDKVLDINKCWLQDDIQNRIRNEIRSYALQHALTFFDLRVQEGFLRTMMV
                     RTTSTSELMLIVVFFYEDKIEREKLLQHLAEKFPEITSLLYVINSKANDTITDQTVHT
                     FKGNDCIYEEMEGLRFKIGPKSFYQTNSDQAYELYKVARNFAGLTGNELVYDLYTGTG
                     TIANFVAHSCRQVVGIEYVPEAIEDAKVNAALNGIENTLFFAGDMKDVLNAEFIRTHG
                     KPDVIITDPPRAGMHEDVVKAMLLAEPERIVYVSCNPATQARDLNLLDEKYRVAAVQP
                     VDMFPHTHHVENVVLLVHR"
     gene            9204..10781
                     /locus_tag="PKH58_06765"
     CDS             9204..10781
                     /locus_tag="PKH58_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88766.1"
                     /translation="MNKKIARLFFIPCFTILSYSCVNSDFDLSNISNNLVLQDETVSV
                     PIGEAKTTIGDLLKKINFENLILSGDSIYLNLRDTMSFVPDNQVEVKSVSVVQDLTVP
                     SLGGHYDFDVNHQFSVSTSGAQTRVDSVRICGTRLKLFVYSGLSNSINLQVVLPEGIV
                     LTNPAQAKITIKPGDNTNYLDISDNSMLKFKNDASNPYFTIRYIVDIPSSGSFALSPL
                     HFDFLFESIKMKVIYGNFPDVNAGEIKKDLYVDFFNDIFTNGSSLHFHNPTIKCMIKN
                     YLGVKTKFNLNNITAYDEKGASASADFNGSPGYTLTLDRSQKLYEPSISYTTFCRSMG
                     NINNFFLIEPKKFTYSVAASTVAEPGENNFIATDKCMDIIMNMQLPFTFDKGSMLIVR
                     DTLNMDISEINKKVKLNDIVLRVNYSNMLPVKSILATVFLDENRKPVGEICEKHMELK
                     NATVDASGYAISESNGTTWLKFDQSETAAISKIKFIVLKVTVAGNDNNTRISLRPSDY
                     IHLKAALYVNGDISIKN"
     gene            10797..12329
                     /locus_tag="PKH58_06770"
     CDS             10797..12329
                     /locus_tag="PKH58_06770"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNX88767.1"
                     /translation="MNKSIVKHLILVVLIMSAFSGLQAQISTMYYLKNNPYRHQLNPA
                     FQPSSGFYIDFPLIPSFEFSLGNNSLTMRDIIYPKLINGEMKTITFLDENGDKDLFYN
                     TLRSQTNFQTNANMDIFSIGFRSGSSYFTFGVSQKFNMNATVPKDMFKLILYGTLDTL
                     NVNRYDMSSMNMSATAYTEYALGYSREINERLSVGLKAKYLKGEANITVGFDKLNLNA
                     DRDKWDATIGGNINGAIPFTKFILSNAGKIDSIASDFPEQSLESGIDTYIDMAKEFVD
                     VINHPSGSGFAVDLGASYKVSDRLSVSVAVLDLGYINWKRNTVNISVSENFNFDGVNF
                     ELDDTTNNYDDYTDIVTDSLQYNTTFNPYKTSLQTKLMAGLEYYIVKDKVSLGLLSKS
                     TFINKKLYEEITTSANFFPSSWFNASLSYSLLNGSFSNIGLGVNASVGPFNMFLASDF
                     IPLRYTPEFVPYKTKSVNLKTGFCLTFGNGKKDKRVKEEEKTNNIENSPNIIPQINPT
                     VN"
     gene            13710..13832
                     /locus_tag="PKH58_06775"
     CDS             13710..13832
                     /locus_tag="PKH58_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88768.1"
                     /translation="MAKQKLQKEKDTAEMISSAKGETDSPVKRTDYKPAGETRK"
     gene            complement(14003..14116)
                     /locus_tag="PKH58_06780"
     CDS             complement(14003..14116)
                     /locus_tag="PKH58_06780"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNX88769.1"
                     /translation="MTVFMIFSYLAFHETIKLNQLIGFLFLILAVYFIFKP"
BASE COUNT         4296 a         2943 c         2746 g         4134 t
ORIGIN      
        1 ctaaccgttt cttatttttt tattgtgaaa taagggcagc aaaatgatcc gcttcaacct
       61 ttttctatta accgccacag aataaagata ccgttcacaa gctaaaacaa agtcatctgc
      121 cctgtgatat cgtcgattat cttctggttg tcatcctccg ggtcaatggg ttcattgttt
      181 tcaggcgttt cgttttcctc atcattttct tccggtttat tatcctcatc cggtttatct
      241 tccgttttaa atctcaacgg atcaagttca ttgatggact cgatttcgaa agttgtcagt
      301 cgttttcctt ttgccttgta acttttcact ccgataaact cttcggcatc caccaccagg
      361 gcttcacgaa atgcatctcc acctccaaac gttacttcga aacgcgggaa atcctcaccc
      421 gacatcagta tcagctgcga agccggattt tcgcccagga agctaaccgg ttttgctgag
      481 ggttccagct gaaaacgctt cagataatga aacttctgat cggcgtcata cagcaccacc
      541 gaccacactt tattcggatc aaacttttcg ataatcagaa tatctttctc gaaatgatta
      601 ctcagatcga aattggtggt gcggtattcg cctttggttg agatgacaag cacctgctca
      661 tcgctcagga attctcccag gtattcgcca cggttatcgt agttgaaacg caggatatcg
      721 cggtcgaacc agacttcgcg tccaccaagg gtagaacctc ctttttgttt caacgcgatc
      781 cggtgcacat cgttcttggt aagcatattt cccatggact gacgaccttt gatggcaata
      841 tcgctgaagt ccttttcgaa aacaagtttt atcaggcggg gctttggctt gagtgttaca
      901 cggataactt ctgcctcagc attcggattg gcggtgaaat agatgatttt ggagccatcc
      961 ttgccctggg taacatcgta ttccttgtcg cgtgtaattc cggttaccgc gaaacgcttc
     1021 atatagtaag gtcctgtttt accgtgacga taaacagcat tgtaaatggt acgcttgtcg
     1081 ttctttttaa agacattgat gtagaggatg ttcttgccca caaaaagctt ttcagctact
     1141 ttcaccactt tatacttacc atctttgtag aaaacaatga catcgtcaat atcggaacaa
     1201 ttgcagacaa attcatcctt tttcaatgct gtaccgataa aaccttcttc gtgattaaca
     1261 taaagttttt cgttggcttc cactacctta gctgctacaa tggtatcgaa attgcggatt
     1321 tcagtatggc gcggatagtt tttgccgtat ttatctttca ggttttcaaa ccagaatatt
     1381 gtgtagtcga tcaaatgatc aaggttgtat tcgatttcct tgattttctt ctgaatgcca
     1441 atgatggtgt cgttagcttt atcgctgtca aatttggtga tacgcaccat cttgatttca
     1501 agcagtttca gtatatcctc gcgcatcact tcgcggataa aatctttttt gaaaggctcc
     1561 agcctcttgt cgatgtgtgc aaccacctca tcctgattcg tactttcttc aaactcctta
     1621 tccttgtaaa tccggttctc aatgaatatt ttttcgagcg aaacgaaaaa cagctgttcg
     1681 ctcagttcat tgcgttgaat gataagttcc tgttcgagta aatctttggt atgatcggta
     1741 ctgatgcgga gcaaatcgct cacgcccatg aaacggggtc ttttatcgtg aataacacaa
     1801 caattgggcg aaatgctcaa ttcacactga gtaaaggcat acagcgcatc aatggtttta
     1861 tccgatgaag ttcccggcga cagatgaata agtatttcca cgttggcaga tgtattgtca
     1921 tccaccttac ggattttgat tttgcccttt tcactggctt tgataatact ttcaatcagt
     1981 ttggaagtgt cggttccgaa ggggatttca gtaatcacca gtgttttatt gtcgatctta
     2041 cctattttcg aacggatttt cactgcacct cccctttcac cgtcgttata acggctgaca
     2101 tctatgcttc cgcctgtttg aaaatcggga tacaacacaa aatcttctcc ccttagataa
     2161 gccacactgg catcgattag ttcattgaaa ttgtgaggta aaatcttcga attcagtccg
     2221 acagcaatcc cttccacacc ctgtgcaagt aaaagcggga attttacagg taaatcaatt
     2281 ggttccttgt tacggccatc gtatgagggt ttccattcgg taattttcgg gttgaaaaca
     2341 gtttcgagag caaatttcga caaacgagct tcgatataac gcggagcggc tgccccgtca
     2401 ccggtgagga tatttcccca gtttccctgg caatcgatca gcaggtcttt ctgcccaagc
     2461 tggaccaacg catcgccaat ggaagcatcg ccatgcggat gaaactgcat ggtatgaccc
     2521 acaatattgg caaccttgtt gtaacgtccg tcgtcgagcc gtttcatcga atggagtatg
     2581 cggcgctgta cgggtttcag accgtcgtat atatcaggta cggcgcgttc cagaattacg
     2641 tatgaggcgt aatccaaaaa ccagttctgg tacatccctg agaggtgatg ttttacagca
     2701 tcgtctgata ttttgggaat tttgtagctt gaatgagtct tattgtctgt ttccaaatca
     2761 tctgtttcca gggttgattc tgctgtttct tcatcaggga tttcttgttc ttcaatgccg
     2821 ttgagctctt cgtcttttgc catatatttt attcaacaat tgattttctg gatcagaaat
     2881 ggtgtttttc cggttcgaaa cctccgaatg tgattgtttt gaccgaatca ataggcaaag
     2941 ttacacaaaa atttgaaaaa tattcagtat gtttgcggtg tattaaggag taaaaaataa
     3001 aagttatcaa caaccatgaa acaactattg aacctgaaat tattgatttt atctgcaatt
     3061 ttattcactt tttcagcaac ttatgcgcaa acagccgaca aaggaaaaat cggatttact
     3121 tattccctga acggaggcac aaatgttctc caacctgccc tgatgggcgc ggcaagtcac
     3181 acggtcacgg atgcttcggg tatagggttg atctatctga gaccaatcaa tcactggctg
     3241 gagttggaaa ccggcatgaa ctattctctg tataaaatta caaccacttc ggcccctact
     3301 ccacaagtca ccacaagcaa ttcaacactg tcgctcattg atattccgat aggtgtaaga
     3361 gccggtttct ggaagtattt ctttgccaat ggtggattac tgctcgatct ggatttaatg
     3421 agcaacagtc atgtagatag ccaaaacggt attggtctga tgctgggcgt gggtgtaaaa
     3481 tacgatttca aattcggcgg ttcactcttt gccaacccct acggaaaaat ccattcgtta
     3541 cttcccttcg gatttgagaa gtaccacgaa cgaatactcg aaagtggctg gaaattcgga
     3601 tttacgtaca aactctgaaa tatattgtat gcttttttga caaagctgta aaaagcgcgg
     3661 cagcaacaca gactgacaac gttatcattt gtatcaaaaa agaaaattca ccatcttatc
     3721 aataataccc ggaattatta ggttattcaa caaaacaacc gattttttaa gtcgttagca
     3781 tcggtttgat ttaataatca atatttttca gtaaatttgc aatctatatt tcaaattgta
     3841 actcaataaa ctgtaaaaat aggatatggc ttcacaagaa gatgttttta aaaaactgat
     3901 tgcacattgc aaagagtatg gatttgtatt tccttcgagc gaaatatacg atggacttag
     3961 cgctgtttat gactacggac aaaacggtgt ggaactaaaa aacaacctga aaaaatactg
     4021 gtgggacagc atgaccctgc tgcacgaaaa cgtagtcgga attgatgccg ccatattcat
     4081 gcatccaacg acctggaaag cttcgggtca tgtggacgcg ttcaacgacc cgttgataga
     4141 taataaagac agcaaaaaac gctatcgtgc cgatgtattg attgaagatt tgctggctaa
     4201 atacgacgaa aagattgaaa aggaagtgga aaaagcagcc aaacgttttg gtgaaagctt
     4261 tgacccggtg atgtttcgcc agaccaatcc gcgggtactg gaaaatcaga ctaaacggga
     4321 tgaaattcat gcacgttttg taaaagcgct aaacgacagc gacctggatg aactgaagca
     4381 aattatcgtg gattacgaag tggtttgccc cataagcggc actaaaaact ggacagatgt
     4441 tcgtcagttc aacctgatgt tttccaccga gatgggttca acggcagatg gagccttgaa
     4501 aatctatctt cgccccgaaa cagctcaggg tatttttgtc aactacctga acgtacaaaa
     4561 aacgggtcga atgaaaatac ctttcggcat cgcgcaaata gggaaagctt tccgtaacga
     4621 aattgtagcg cgtcagttta ttttccgcat gcgtgaattc gaacaaatgg aaatgcagtt
     4681 cttcgttcgc ccgggagaag aaatgaagtg gttcgaattc tggaaacaat tccgcttgcg
     4741 ctggcacaaa gcactcggac tgggagatac aaaatatcgt ttccacgacc acgataagct
     4801 ggctcactat gccaacgccg cgacggatat tgagtttgaa atgccgtttg gcttcaagga
     4861 agtggaagga attcattcac gcaccgattt tgacctgggc agccatgaga agtattccgg
     4921 taagaatatc aaatacttcg atccggaact gaatcagtca tacactccgt atgtcatcga
     4981 gacttctatc ggcgttgacc gtactttcct ttcgataatg gcagccgcct acaccgaaga
     5041 agaacttgaa ggtggagaca gccgggtagt acttaaactg ccgcctgtac tggctccgat
     5101 aaaagctgcc attttacccc tggtgaaaaa agacggactg cccgaaaaag ccagagagat
     5161 catgaatgag cttaaatttg atttcaaatg cgcttatgat gaaaaggaca gcatcggtaa
     5221 acgttaccgc cgccaggacg cgattggaac accattctgt attaccgtgg atcatcagac
     5281 actcgaagac aacactgtta ccatccgtca ccgcgacgat atgacacagg aacgcatagc
     5341 aatgtcggaa ctgaataaaa tcatcggcga ctatgtgaac atgaaaaatt tgttccgcaa
     5401 aattgtagac taaatcacag ctttccatat atacaaaaag cctgattttc agaaatgaaa
     5461 atcaggcttt tttattattc aaaccatttc tgatttttgt aattgtgtcc gggtttcaca
     5521 cctcataatc cacacaaacc ctgcctgttt taactttacc aatataagcg cccacttttt
     5581 tgtgttcggg atgctcctgg tagatgttca ggtcatcgat actgtcaaat tcacagtaaa
     5641 gtacaatatc ataattggaa acaggtgtat cagggtgatt aatacccact tcaatttttt
     5701 ttgcctgcgg aataagattt accaggtttt ccagtccttc ttttataatc tgagcatttt
     5761 ctgctttggt ttttccttcg gcttcatcag ccagctgaaa acatacaatg tgtttgatca
     5821 tacttgttca aatttatttt ttctttttag tgtattcctg cgcatttttt tcttcttcca
     5881 tttttttcgg ataatctacg gtataatgta gtccacggct ttcctttctt gcaatggctt
     5941 gcttgataat caggtatgaa acactgatca cgttacgcag ttcgcatatt tcacgcgaaa
     6001 ccacagagcg gttaaacagg ctttcagttt cccgaaagag tatttccaga cggctcatgg
     6061 ctctctgcaa acgcagattg gagcgcacaa taccgacata ggtactcatg atttgctcca
     6121 cttccctgaa actttgggta atcagcacca tttcttcggt cagagaagtt ccttcatcgt
     6181 tccagtcagg tattatttca ttgaaagtca actgatccag acggggtgca gcatgcttca
     6241 cagcttcctc tgcatagaca atggcttcaa tcaacgaatt ggaagcaagg cggttggcac
     6301 catgtaatcc ggttgccgaa cactcgccca gcgcataaag ccggttgaca gaagactgac
     6361 catgaatatc gacggcaata cctccgcaaa tatagtgctg agtgggacaa atagggatat
     6421 agtctttggt aatatcaata ccatattcca ggcatttatg ataaatcata gggaaatgtt
     6481 ctttcgtttc ctccgcattt ttatgggtta cgtccaggta ttcgaaactg tcgccatgta
     6541 ttttcatttc gttgtcgata gcccttgcca ctatatctcg tggtgccagt gagccacgac
     6601 tatcgtattt ctgcataaat tcctgtccgt cgcggccacg aagtacagca ccatagccac
     6661 gaatagcttc agtaataaga aataccggtt tgtcgccggg atggtacaaa gctgtgggat
     6721 gaaactgtac aaattccata tctctgatta ctccccgcgc ccggtgaacc atcgccacac
     6781 catcaccagt agcaattgcc ggatttgttg atgcctgata cacacttccg ataccgcccg
     6841 ttgcaagtat cgttactttt gataggaatg tgtggatatg attacttttc tggttaagcg
     6901 catatacccc gtaacattca atgtcgggtg tatgatgtgt tacaatttcg cccagatggt
     6961 gctgggttag tatttcgatg gcaaaataat gttcatacaa atcaattccg ggatgattac
     7021 gaatctgctt attcaggctt tcctgaataa taaagccggt attatcctta tgatgaagta
     7081 tcctgaattc agagtgtccg ccttccttgt gaagatcgaa ttgattgcca tcttcggttt
     7141 tatcaaaatc catcccccac tccagcaact ggtcaatctg tgcccgcgca tttttcacaa
     7201 ccaattccac ggctgtaata tcacagtgtc catcacctgc aataagagta tcctctatgt
     7261 gttttttgta attatccggt tcatacataa ccgtagctat acctccctga gcataggatg
     7321 tattgctttc ttccagcgtt gttttacaga gcagcgccac tttaccatag cttgcagctt
     7381 tcagggcaaa actaattcct gcaatgcccc ctcctactac gacgaaatca tattttctgg
     7441 ccataatata tcagtaaaca agtttataga taacaagtaa gtgtgttaaa aaatttttag
     7501 ctatcggtga actaacagca cgacattctc cacatgatgg gtgtgcggga acatatcaac
     7561 cggctgaaca gcagccacac ggtatttttc gtccagaagg ttcaaatcac gagcctgagt
     7621 agccggatta caactcacat atactattct ttcaggttct gccagcagca tggctttcac
     7681 cacatcttcg tgcataccgg cacgaggcgg gtcggtaatg atgacatcgg gctttccgtg
     7741 tgtgcgaata aattcagcat taagaacgtc tttcatatca ccggcaaaga aaagcgtatt
     7801 ttcaattccg ttcaaagcag cattgacctt cgcatcttca atggcttcag gaacatactc
     7861 aataccaacc acctggcggc aactatgtgc tacaaaattg gcgatggtac ctgtaccggt
     7921 gtacaggtca taaaccagtt cgttgccagt cagtcctgca aaattacggg caaccttata
     7981 aagttcataa gcctgatcag aattcgtctg ataaaacgat ttcggaccta ttttgaaacg
     8041 taatccttcc atctcttcgt atatgcaatc gttgcctttg aaggtgtgta ccgtttggtc
     8101 ggtaattgta tcattagctt ttgagttgat tacatagagc aatgaagtga tttccggaaa
     8161 cttctcggcc agatgttgca gcagtttttc cctttcgatt ttatcttcgt aaaaaaagac
     8221 aacaatcagc atcagttcgc tggtggaggt agtacgaacc atcatggtac gcaggaaacc
     8281 ttcctgtacg cgtaaatcga agaatgtcaa cgcatgctgc aatgcatagg agcgaatttc
     8341 attcctgatg cggttttgaa tatcgtcctg caaccagcac ttattaatat cgagcacctt
     8401 atcaaacatg cccggaatat gaaaaccaac cgcgttcatg gtgtcaaatt cttttgcaga
     8461 gttcatttcc tcaaaagttc tccagcgtcg gttggaaaag gtgaactcaa gtttattacg
     8521 atagaagatt gttttagcgg aacccagaat tggagagatt tcaggcagtt gaacctttcc
     8581 gatccttcgc aggttatttt caacttcgtt ctgtttgtat tttagctgtt cagcatatgg
     8641 taatatttgc catttacatc ctccgcaaat tccaaaatgc tcgcaaaaag gttcctgcct
     8701 gatttcagaa ggtttcacaa tatcaaccac gcgtgcctcc ataaaactgc ttttcttacg
     8761 tgtaacctgc aaatcagcaa catcaccagg tactgcaaac ggcacaaaca ccacaatatc
     8821 attcactttt gcaatagctt ttccttcagc agcaatgtca gtaattgtta cattttccag
     8881 gagtggaagc tgttttttat tttttgccat atatagtttt ttgtgttgca aaggtaaaga
     8941 tttttttccg gtaatcattg ataaaagggg ttatgttaaa taattcgact ttaacttttt
     9001 cgaatgatta atttgattgg acttaattat taattttaat aactttgtgc tttatcatta
     9061 cgataaacgt taattttaac cttaaatatt tttagacaac aacaaacatc taatcagtat
     9121 aacaaacttt ttaaaaacaa gttttattat ttcaatttgc aacaacaacc agatcaatac
     9181 aactaaaatc acacaaattc attatgaata aaaaaattgc ccgattgttc tttattccat
     9241 gttttacaat tctttcatac tcttgtgtaa attcggattt cgacctcagt aacatttcca
     9301 acaatctagt acttcaagac gagacggttt cagttccaat tggtgaagct aaaacaacga
     9361 ttggagacct gttaaaaaaa atcaactttg aaaatcttat tctttcgggc gacagtattt
     9421 acctgaacct cagagacacc atgagttttg tacctgacaa tcaggttgag gtaaagtccg
     9481 tatcagttgt tcaggatctg actgttccct cgctgggagg acattacgat tttgatgtta
     9541 accatcaatt ttccgtaagc acatcagggg cacagacacg tgtggatagt gtgcgcattt
     9601 gtggaaccag gcttaaactc ttcgtttatt ccggattgtc gaattctatc aatcttcaag
     9661 tggttttgcc cgagggaatc gtactcacaa accctgcgca ggcaaaaatc accatcaagc
     9721 ccggtgacaa cacaaattat cttgatatca gcgacaattc gatgctgaaa ttcaagaacg
     9781 atgcctctaa tccatacttc accatcaggt acatcgtgga tataccttca tctggctcgt
     9841 ttgcgctttc tcccctgcat ttcgattttt tatttgaatc cataaaaatg aaggttatct
     9901 acggcaattt cccggatgta aatgcgggtg agattaaaaa ggacctttat gttgatttct
     9961 ttaacgacat tttcacaaac ggcagttcac tgcacttcca caatcctacc atcaagtgca
    10021 tgattaaaaa ttatttaggc gtaaaaacca aattcaactt aaataatata actgcctatg
    10081 atgaaaaggg agcttctgcc agtgctgatt ttaacggttc tcccggatat acattgacac
    10141 tggacagatc acaaaaactg tacgaaccca gtatttcata tactactttt tgccgctcca
    10201 tgggcaatat aaacaacttt tttctgatag agccaaagaa atttacttac agtgtagctg
    10261 ccagcaccgt tgctgaaccg ggtgaaaaca attttatagc aactgacaaa tgtatggata
    10321 taatcatgaa catgcaactt ccgttcactt tcgacaaagg gagtatgtta attgtcaggg
    10381 acacgctcaa catggacatt tctgaaataa acaaaaaagt aaagctaaat gatattgttt
    10441 taagggtgaa ctattcaaat atgcttccag taaagtcaat tcttgccact gtgtttctgg
    10501 atgaaaacag aaaaccggta ggtgaaattt gcgaaaaaca tatggaattg aaaaatgcta
    10561 ccgtggatgc cagtggttac gctatttcag agtcgaacgg cactacctgg ttaaaattcg
    10621 atcaatcaga aactgccgct atttcaaaaa taaaatttat tgtacttaaa gttactgttg
    10681 caggaaatga caacaataca cggatttcat tacgtccgtc agattacata catcttaaag
    10741 ccgctttgta tgtaaacgga gatatatcaa tcaagaacta aacaccgtaa gaaaaaatga
    10801 ataaatcgat agtgaaacac ctcatattag ttgtattgat aatgtctgct tttagcggac
    10861 tgcaggcaca gataagcacc atgtattatc tgaaaaacaa tccatacaga catcagttaa
    10921 atccggcatt tcagccatca tcaggctttt acatagattt cccactgata ccttcgttcg
    10981 aattcagcct ggggaataat tcgttaacaa tgagggatat tatttatcca aagttgatta
    11041 acggagaaat gaaaactatc acatttctgg atgagaacgg cgataaagat ttattttaca
    11101 acactttaag atcgcagaca aactttcaaa caaatgcgaa tatggacatt ttttctattg
    11161 gtttccgttc gggaagttcg tactttacat ttggtgtttc gcagaaattc aacatgaatg
    11221 ctaccgtacc aaaagatatg ttcaaactga ttttatacgg tacacttgac acactaaatg
    11281 taaaccggta tgatatgagt tcaatgaata tgtcagcgac tgcttatacc gaatacgcgt
    11341 tgggttattc ccgggaaatc aatgaaaggt tgagcgtagg tttaaaagca aaatacctga
    11401 aaggcgaagc aaatattact gtcggattcg ataaactaaa tcttaatgct gacagagata
    11461 aatgggatgc aaccattggg ggcaacatta acggagcaat accattcacc aaatttattc
    11521 tgagcaatgc cggtaaaatc gactccattg cctccgattt tcctgaacaa tccttagaat
    11581 ccggcataga cacttatatt gacatggcta aagagtttgt ggatgttatc aatcatcctt
    11641 ccggaagtgg ttttgctgtt gacctgggag cttcttataa agtatcagac cgactttcag
    11701 tttctgttgc cgtgctggac cttggatata tcaactggaa aagaaatacg gttaacatct
    11761 ctgtttcgga aaatttcaat ttcgatggag ttaatttcga attggatgac acaaccaaca
    11821 actacgatga ctacaccgac attgttacag acagtctgca gtataacacc acattcaatc
    11881 catacaaaac atccctgcaa accaaattga tggccggatt ggaatactat atcgtgaagg
    11941 acaaggtaag tctcggactt ttgtcgaaaa gcacatttat caacaaaaag ctttatgaag
    12001 aaataaccac ctctgccaat ttcttccctt caagctggtt taatgcttca ctgagttatt
    12061 cattgttaaa cggcagtttc agtaatatag gacttggagt taatgcctct gtgggtccat
    12121 tcaatatgtt ccttgccagc gattttattc cccttcgata tacaccagaa tttgtaccat
    12181 acaaaacaaa atctgtaaat ttaaaaacgg gcttctgcct gacattcgga aacggaaaaa
    12241 aagacaaaag agttaaagag gaagaaaaaa ccaataacat tgaaaattct ccaaatataa
    12301 ttccccaaat aaatcctacg gtcaattaat ttatccattt tgaaattaaa attcaccttt
    12361 attgtaaaaa ttggttgttg ataactctcg atttttattc ggcaaaaagt acttgtatct
    12421 gttcattgca ggctgtttga gtagatgttg ataagtctgt tgataactaa gctgaaaact
    12481 tgttgatata aacaggtaaa ataatttgca aatcgattat aaggtgtatt atatttgcga
    12541 catgagttat tactcatagc attaataata gatgcgaagt gatctttgaa aaactgaaac
    12601 accaaagaac aatcaatact atttaatatc cgcaaccgga ttttccggag aaaatttaca
    12661 tgcaaaacga aggatgcact gactataagc atcgttatct tccaaattgg atacaagccg
    12721 tgtccaatgt gtagtatagc aaagccgatg gttttacgat tcttggcagc atgcaaatac
    12781 ccgaaaaatc cgactttcaa ctaaatacgt ttcactgaaa cgcaactttg cttgacgaaa
    12841 gcagggacgc cgtgttgtcg aagcagaata aagtcctttg tatttcgata caaatacttt
    12901 ttccacaggg ctaaaagtga aaacagcaat gtttttacgt tgcgactgaa ggtttcaggc
    12961 gacgcagcgc aaaaaaaatt aaaagtcaaa tcaaggttca ggtattagaa aattgaaaaa
    13021 gtgttttctc ataaaaacat ttttaatctt ggtgaaaacg cagagagaat aatttacttt
    13081 gaagatctgc aaaaaagtaa ttgcaggaat gattgccgat agataaaatc gggtttcaaa
    13141 atactgcaaa agacgaagag gtaacaacct gcagaaggca caaaagcaaa cagtctcgca
    13201 aaacaggaag aaaatgtaag ttttatcaaa aaggactgaa atcaatccgt acgatgcgaa
    13261 acgaaagtct gcccgaaaaa ttgatggtaa gatcagttaa ccggtaagct cgtaaaaaag
    13321 caacagacat cgattaaccc tgtgatgaag tgatataacc ataacaggga atattgccac
    13381 acatgccgaa taaataacgg taaaaaatag gataatcgac acaatttgcc tgtgtaccaa
    13441 ctacttcatc caccaccata taccccgaat aaaaaattct gaggaaaagg tagcgactaa
    13501 aatgcattga ctaaaaagta ataaagtgaa tatccacaga gtagcggtga ttttactgaa
    13561 aatcatgaaa caagggatta tgagttagaa cacctgcata gcccacttaa taaaaacgaa
    13621 aaaagagtaa tcttatcgtt tgaaactaat ctgaacggag aattgtaata aagtagtttc
    13681 gaatagaaat tgctcacaat aaaccggaca tggcgaagca aaaactacaa aaggaaaaag
    13741 atacggctga aatgatttca tcagcaaaag gcgaaacaga cagtcctgta aaacggacag
    13801 actacaaacc ggctggtgag acaaggaagt agaagtaaag tagaaacaac atacgaaaaa
    13861 cgcatcctat tcacacttaa agcgtacaaa cagttattca ggaatactac acatcaatta
    13921 taatgttcaa agagggtacc cgaaaaggta ccctctttca tttttaaatg gctgagaatg
    13981 tatttatacg ttttactgat actcagggtt tgaaaataaa atacacagct agtattagaa
    14041 aaagaaaacc tatcagttga tttaatttga tggtttcgtg aaaagccagg tatgagaaaa
    14101 tcataaaaac agtcagtgt
//
LOCUS       DAOPYB010000067        13918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4806_length_13918_cov_9.473058, whole genome shotgun sequence.
ACCESSION   DAOPYB010000067 DAOPYB010000000
VERSION     DAOPYB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4806_length_13918_cov_9.473058"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKH58_06785"
     CDS             complement(<1..382)
                     /locus_tag="PKH58_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88770.1"
                     /translation="MTTTKKLRIVYIKPNDTSFIRNDQLIFEKYYEVIPVLLSQSAGK
                     IRYGINNIKLFFRLLASGMRKDTLFVAWFADYHSAVMALAGKLTGRKTVVLIGGQEAV
                     CYPELNKGVYRSKFRGAFVKYALRN"
     gene            504..1349
                     /locus_tag="PKH58_06790"
     CDS             504..1349
                     /locus_tag="PKH58_06790"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX88771.1"
                     /translation="MKILVTGYSGFIGSYLIRRLQDLQVELILCDLQNGINICNWNEV
                     KDLKGFDIIIHLANLSFVPASFRDPKKFYEVNYLGTLNMLELCRLNKARMIYFSSYMY
                     GNPQYQPIDEQHRIEAYNPYAQTKVICESLCEGYNRDFQVPVTVFRPFNIYGKGQNPD
                     FLIPTIINQAKTGKIVIKDDRPKRDYIHVTDIVEAVILALQTPNLQPTLQTYNLGTGE
                     SHSVKEIIEIVRTLSEHEVDYECTREIRPNEVLDTVADITKIKKELHWTPKISLIEGI
                     KSMMD"
     gene            1365..2339
                     /locus_tag="PKH58_06795"
     CDS             1365..2339
                     /locus_tag="PKH58_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HNX88772.1"
                     /translation="MKNILVTGAGGFIGSHLTELLVAEGYQVKAFVHYNSWNKWGWLD
                     SSPIRNKIQVVTGDIRDYDSVFAAMQGCDTVFHLAALIGIPYSYVSPQAYIKTNIEGT
                     YNVLQAARLLNIEKLMVTSTSETYGTAQYVPIDENHPMVGQSPYSATKIGADQLSVSY
                     FKSFDLPVKIVRPFNTYGPRQSARAIIPTVATQILNGSRSLKLGNLAPTRDLTFVKDT
                     AKGFLEIAKADGLYGEITNIGMSEEITIGNLVKLIANIIGTEVEIIADEQRIRPDKSE
                     VERLYCNNTKIKASTNWTPDYTLETGLAETINWIRENMSYFKSDIYNV"
     gene            2332..3036
                     /locus_tag="PKH58_06800"
     CDS             2332..3036
                     /locus_tag="PKH58_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNX88773.1"
                     /translation="MSKRAVILAGGKGTRLKPYTVSLPKPLVPIGEMPILEIIIRQLA
                     KSGFDHITITVNHMADIIRAFCGDGNKWGVTIDYSLEDKPLSTMGPLKLIQDLPENFL
                     VMNGDVLTDLDFGQFYDEHVRSGNIFTVSAFTRDQKVDYGVLEIGDDNKLSDFIEKPT
                     NRYNVSMGVYMASNKVLEYIPENQFYGFDHLMLDLIKDQHPATVKIHSGYWLDIGRPD
                     DYEKACKDFTENNFVF"
     gene            complement(3043..4035)
                     /locus_tag="PKH58_06805"
     CDS             complement(3043..4035)
                     /locus_tag="PKH58_06805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX88774.1"
                     /translation="MKPKVSVVVPVHNAGKFFSRCLDSLIGQTLTDIEIILVLDTPTD
                     GSDKVAEQYAQQDKRIRIVRNTTNLHIGYSRNAGIDVAEGDYVGFSDDDDVCHPRMFE
                     VLYEKAQTCHADIVVSNYCNEVNGKVENYCFPEQLSEKEFKQKYLEALIRGNHSQPDT
                     VSFNNVNPVWNQIFSRELLNQNNIRFTDNRKVSMEDVIFNIQAHFYARKVEFVPQVFY
                     RHIINEKNAFNNYDYLSHEKVTAHIHFVHRFMEKNGMLSEYRDLYLICIIKRVYTSFR
                     NEIRFKRIFHAIPFIRKIRNNADLQQAISPLFEKKQLLNKLPVTKRLFAFLIRK"
     gene            complement(4038..4700)
                     /locus_tag="PKH58_06810"
     CDS             complement(4038..4700)
                     /locus_tag="PKH58_06810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX88775.1"
                     /translation="MSDKSSIQIKTGIGYYQNQRKEMLEFIPENAKFILEVGCGEGVF
                     SSMMSREDREIWGIEINPQSAELARKKCKQVFVGDFNEIYGQLPRNYFDCIVFNDVLE
                     HMYAPWDVLRNVKNLLTPQGVVVSSIPNFRYISNLITEILFQGEFRYRPEGGILDDTH
                     IRFFTSKSILRMYQEQGYKVLKHQGINSCKSPKEKLFIRLFLGKLNDIRYKQFATVAK
                     PE"
     gene            complement(4752..5390)
                     /locus_tag="PKH58_06815"
     CDS             complement(4752..5390)
                     /locus_tag="PKH58_06815"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HNX88776.1"
                     /translation="MKLIKLFFALLCLIPAMQGQAQTIDKYFVDMPAILMPGLESKMR
                     LELTEYYKSERRDSIRNSYGYQVKLLTLDTVNNYISVQAASNTRFELKTFQRSDTSVV
                     IAVIHTICGPVCSSYIHFYDKGWNEIKIDFPKLNNRNWLKTPESIVDGEKVEDMLKAT
                     FIELTFKQEKNAIEARNHSFEYLNLESGKLLEPYLKKENIIITFENNGWHIQ"
     gene            complement(5377..9822)
                     /locus_tag="PKH58_06820"
     CDS             complement(5377..9822)
                     /locus_tag="PKH58_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNX88777.1"
                     /translation="MLGIIFLLALAVVAFRNNGVQNYLAGQVTRQLSEKLHTRVSIGK
                     IDYRYFNSFRFDSLYIEDQQRDTLIFVERGYAGFDFWKFFSGKYVFKEIKIEGLYGNL
                     VIDTAGVSNLDFVIKAFSKPQKPKKESKVEFNFKNIRLNDSRFRLTNLQHPAASDANR
                     FDGNRMDFRHLNLVLNVDYLKKDSLSARIRSFSAVERSGLTISNIETVVHGWKKGFHM
                     PFLNVSLPHTSLLLDSVSMTYDSVANLRNMLNKVKWNARIKTSKVAMKDLAAFAPNFR
                     YMKENASVRGLLTGRISSFRLHEMEMNYGKSLTLKADLDLNGIPNLDETFIYANIRDF
                     HVNKGDAQDFISQLSHKPFVLPKELSRLGVVRYTGNISGFFSNLVAYGNITTNAGSLN
                     TDILLQFENKLSDLKYNGTIRTQNFQLGQVLNTKLFGKTAFKITTKGTKLHNRSLQGT
                     ISGDIAEIYLNKYNYQNISLDGNYDGTGFEGKMDVKDPNLDAGFDGMVDLTKKLPVFN
                     FDLQVNNADIHALHLTKEYKDSQLSFDGNTNMVGNSPDNLNGYLMLNNIRFLNAGEQL
                     VMDQLQFVSEVGAKSSKFTITSDLINGNFEGDFKYSTIPQTLSQIVENYIPALSGKNI
                     TGKRKQVNANNYMNIDLTLSDTKLISRVLNLPFTLDGITTVKGFVDDINERVELNART
                     PFLSFGSQKIRNIELLLNNEHQQLNLQASARLENQKNLTNVSLKAAAANDLLYTQFGW
                     QNNDTINYAGEIQTNTKFNKENNLTSALVTFLPTQIVVADSTWDILGSQVSINPDTTF
                     TVDNFRIKNRKQYINLDGTVSRKETDILTVDMNDLNLDFLMALVKLKNVSIKGNATGK
                     AEVYSILKQPVFEADIEVKNARLNNSPIGNAYLYSTWDRENKELQASGTFIDGKDTVA
                     LANGVYIPAKDSIDFIYDAHRLNLGFLTSWLSSFSKEVTGLGTGKVRMFGKTKSIGFE
                     GSVFAENAKLSVEYLKTSYSFTDSVYLTRKSIALKNITVYDEEKNRGYLNGLITHDGN
                     FNNMKYDLKINARNMLALNTQPRDNEFFYGKAYASGNVNIYGNMANIYIDVNASTQPK
                     TKVFISVGGAAIANENNFITFVNQKDTTKTKTKDTVVSEAKPTRLRMNLMVDVTPDAE
                     IQLIIDPKAGDMLSGTGSGSLRMEYDDFSDIKLYGTYTIDEGKYLFTLQNLLRKDFKI
                     NQGSNISWTGDMLRAQMNITAIYSQTVSLRDLMNDAILSSTSRTSVPVNSVLKLTGDL
                     MTPDIKFDIDLPTSDETLKMQVRNLINTEEMMNRQMMYLLLFNKFYTPDYAKGAATFA
                     SSLGNNTWSLFANTAFGQINNWLSQMTNTFQFGANIRSTGLGSESAQEYEAEISYQPN
                     NRLLVNGNLGYRNDDLAKNKIIGDVDVEYILTENGKLRLKAYNHTVDRYTLRSAPFIQ
                     GVGVMYKETFNTWDELLRHYFNVLTKPAKTPADTTKTEPTNNETN"
     gene            10095..11129
                     /gene="tsaD"
                     /locus_tag="PKH58_06825"
     CDS             10095..11129
                     /gene="tsaD"
                     /locus_tag="PKH58_06825"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNX88778.1"
                     /translation="MSENITIIGIESSCDDTSAAVIRNGVMLSNVIAGQAVHTRYGGV
                     VPELASRAHQQNIIPVVHDALVQAGIDKKELTAIAFTRGPGLLGSLLVGTSFAKGFSQ
                     ALNIPLVDVNHLQGHVLAHFISQGKEDEKFPSFPFLCLLVSGGNSQIVLVRNYLDMEV
                     IGQTIDDAAGEAFDKCAKVMGLPYPGGPHVDRLAKEGNSKAFKLNKPHLQGYDYSFSG
                     LKTSFLYLLRDEIRKNPNFVQENMADLCASLQATIIDILMKKLRKAADDLNIKQVAVA
                     GGVSANSGLRDAFHQHAEKYGWDIFIPPFAFTTDNAAMIAITGYYKYLKGEFCSIEEV
                     PYARVSNLLV"
     gene            11135..11722
                     /locus_tag="PKH58_06830"
     CDS             11135..11722
                     /locus_tag="PKH58_06830"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HNX88779.1"
                     /translation="MKLQLPEKLKPYSGVIYFVIILLVSHFFWKYTVLGDEEGYLVSF
                     FGMDITLPFDFMARHTARVSHWLLQGLGFSTSLTDNNVIRHTVSLNAVHIVWSCTGIK
                     QAYILFCILAFSRGPWQHKLWYIPLGLLCVYLFNILRITFITAIMDGHPEQFELWHEH
                     ITKYAFYIMIFGIWVVWEEKIRLFGIRSQVQQTNA"
     gene            complement(11804..12901)
                     /gene="buk"
                     /locus_tag="PKH58_06835"
     CDS             complement(11804..12901)
                     /gene="buk"
                     /locus_tag="PKH58_06835"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNX88780.1"
                     /translation="MHKILVINPGSTSTKIAVYEEEQQQSVFTIRHSTTEVTQYEHVL
                     DQFDFRYQLIIQTLRDNGVNMSDFSVIMGRGGMLPPVDSGVYEVNDLMLDTLRKHERG
                     EHASNLGAFLAEKIASAIPGCKAYIADPVAVDELDDVARISGMPEIPRRTIFHALNHK
                     ATARLHASIVGKRYEDLNLVVAHLGGGVTIGAHRKGRVIEVNQGLDGWGAMSPERAGT
                     IDAGLLVRMCFTGKYTQHEILRKLAGQGGLIAHCGSNNVQELVKKAESGDEQVAKVLQ
                     AFAYSVGKEIGAMLAVLHGKTDGVILTGGVAYNDYIVDYVKAMIEPLATVYVYPGEDE
                     MAALASNAMRILRGESPKVFSVKKGYVDYNF"
     gene            complement(13058..13588)
                     /locus_tag="PKH58_06840"
     CDS             complement(13058..13588)
                     /locus_tag="PKH58_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNX88781.1"
                     /translation="MSNRNNKILIAYFSRRGMNYQNGNVVNLLVGNTEIIARKLQKMT
                     GGDLFEIRTAVDYPRDYMETTRIARNELRTNARPILAEYIENLENYNVIYLGYPNWWG
                     TFPMAVCTFLEYASFNGKTIIPFCTHEGGGFGHSETDIRKLCPDAILLPGIAIQGGSA
                     KTADVQLEEWLKKSNS"
BASE COUNT         3900 a         3011 c         2893 g         4114 t
ORIGIN      
        1 cattgcggag ggcatatttt acaaaagcac cgcgaaattt gctgcggtac actcccttgt
       61 tcagttccgg ataacataca gcttcctgtc cgccaatcaa aaccaccgtt ttgcgtccgg
      121 tgagtttacc tgcaagtgcc atcacagccg aatggtaatc ggcaaaccag gctacaaaga
      181 gagtatcctt tctcataccg ctggccagca gtctgaaaaa aagtttgata ttattgatgc
      241 cgtaccggat cttacctgcc gactgagaga gcaatacagg tataacctcg taatacttct
      301 caaatatcaa ttgatcgttg cggatgaagg atgtatcgtt gggtttgatg taaacaatcc
      361 ggagtttttt tgtagtggtc atactgacga aatttgctgt ttcagccaac aaagataaac
      421 aatcttcaca gaattaagta tcggaaaata aaaatcaggt tttttgttta actttgtgac
      481 tcgaaagtaa aaataacccg taaatgaaaa ttctcgtcac cggctattca ggttttatcg
      541 gaagttatct gatcagaaga ttgcaggacc tgcaagtcga actcatactt tgcgacctcc
      601 aaaacggcat aaatatctgc aactggaatg aggtgaagga tttgaaaggt ttcgacatta
      661 ttattcacct ggctaatctt tcattcgtac cggcatcgtt ccgcgatccg aagaaatttt
      721 atgaagtcaa ttacctgggt acgctcaata tgctggagct ctgccggtta aacaaagccc
      781 ggatgattta cttcagttct tatatgtatg gtaatcctca gtatcagcca attgatgaac
      841 aacaccggat tgaagcatac aatccgtatg ctcagaccaa agtaatctgc gaaagcctgt
      901 gtgaaggtta caaccgcgat tttcaggttc ccgttactgt tttcaggcca ttcaatatct
      961 acggcaaagg tcaaaatcca gattttctga taccaaccat tatcaatcag gcaaaaacgg
     1021 gtaaaattgt aataaaagac gacagaccaa aaagagatta tattcacgtt acagacattg
     1081 ttgaagctgt aattctggca ttgcaaacgc caaacttaca accaacactg caaacttaca
     1141 atctgggaac cggcgaaagc cattcggtca aagagattat cgaaattgtc aggacacttt
     1201 ccgaacatga ggttgactat gaatgtaccc gcgaaatccg tccgaatgaa gtgctggaca
     1261 cggttgcaga tatcacaaaa ataaaaaaag aactacactg gacaccaaaa atttctctta
     1321 ttgaaggaat aaagtccatg atggattgaa taatacaaac gaaaatgaaa aatatacttg
     1381 taaccggagc cggaggtttt atcggctcac atctcactga attgctcgtt gctgaaggct
     1441 atcaggtaaa agcatttgta cattacaact cctggaacaa atggggatgg ttggacagca
     1501 gtcctatcag aaacaaaata caggtagtaa ccggcgatat ccgcgattac gattcggtat
     1561 ttgcagccat gcagggctgt gatacggttt ttcacctggc tgcacttatt ggtattcctt
     1621 attcgtatgt ttcaccgcag gcttacatca aaacaaatat tgaaggaact tataacgttc
     1681 ttcaggcggc aagactgctg aacattgaga aactgatggt aacatcaacc agtgaaacct
     1741 acggaaccgc acaatatgtt ccgattgatg aaaaccatcc tatggtcggc caatcaccct
     1801 actcggccac aaaaatcgga gccgaccaac tttcggtaag ttatttcaaa tcattcgacc
     1861 ttccggtgaa aattgttcgc ccgttcaaca cttacggacc ccggcaatca gccagagcta
     1921 ttataccaac cgttgccact cagatactca acggcagcag gtcgctgaaa ctgggcaatc
     1981 tggctcctac ccgcgacctt acctttgtca aagacaccgc aaagggcttt ctggaaatag
     2041 ctaaagccga cggactttat ggtgaaatta caaatatcgg gatgagcgag gaaatcacca
     2101 tcggcaatct ggtaaaactt atcgcgaata taattggaac agaggtggag attattgctg
     2161 acgaacagcg catacgaccc gataaaagtg aagttgaacg gctatactgc aacaatacca
     2221 aaatcaaagc cagcacaaac tggacgcctg attacacact cgaaacaggg ttagcggaaa
     2281 ccatcaactg gataagagaa aatatgtcgt acttcaaatc tgatatttac aatgtctaaa
     2341 agagcagtca tactcgcagg cggaaaaggc acacggttaa aaccctacac ggtaagttta
     2401 cccaaaccgc tagtaccgat aggtgaaatg ccaattcttg aaatcattat tcgtcagctt
     2461 gcaaaatcgg gttttgatca catcaccatt accgtgaacc acatggccga tattatccga
     2521 gctttttgcg gcgacggcaa caagtggggt gttaccatag attactcgct cgaagacaag
     2581 ccgctgagca ccatgggacc cctgaaactt atccaagatt tgcccgaaaa ctttctggta
     2641 atgaacggag atgtactaac cgatctggac ttcggacagt tttacgatga gcatgtacgc
     2701 tccgggaaca ttttcaccgt ttcagccttt acccgcgacc aaaaagtgga ttacggtgta
     2761 ctggaaattg gtgatgacaa caaactgtcg gattttatag aaaaacccac caaccgatat
     2821 aatgtgagta tgggagtata tatggccagt aataaagtac ttgaatatat tcccgagaat
     2881 cagttctacg gatttgatca cctgatgctc gatcttataa aagaccagca tccggcaacc
     2941 gtgaagatac actcgggtta ttggctggac atcggccgac ccgatgatta cgagaaagcc
     3001 tgcaaagatt ttactgaaaa taattttgta ttctgaaggc tgctattttc gaatcaggaa
     3061 agcgaataac cttttcgtta caggtaattt attcagcagt tgttttttct cgaaaagcgg
     3121 acttatagcc tgctgtaaat cagcattatt ccttattttc ctgataaacg ggatcgcatg
     3181 aaagattcgc ttaaatctga tttcatttct gaaagaagta taaacgcgtt tgatgataca
     3241 aatcaagtac aaatcccgat attcacttaa catcccattc ttctccataa agcgatgcac
     3301 aaaatgaatg tgtgctgtca ctttttcgtg cgagaggtag tcgtaattat taaacgcatt
     3361 cttttcattg ataatgtgcc ggtagaatac ctgcggaaca aattccactt tccgtgcata
     3421 aaagtgtgcc tgaatattga agataacatc ttccatagat actttcctgt tgtcggtgaa
     3481 gcggatattg ttttgattca gtaattcacg actgaatatc tggttccaaa ccgggttcac
     3541 gttattaaaa gaaaccgtgt ccggctgcga gtgattgccc cgtatcagtg cttcgagata
     3601 tttttgctta aattcctttt cggacagttg ttccggaaaa caataatttt caaccttacc
     3661 atttacttcg ttacagtagt tgcttacaac aatgtcggca tgacaggttt gagctttttc
     3721 atataatact tcaaacattc gcgggtgaca tacatcatca tcatcggaga aacctacata
     3781 atcgccttca gcaacatcaa ttcccgcatt gcgcgaatat ccgatatgca gattggttgt
     3841 gtttcttaca atacggatac gcttatcctg ttgtgcatac tgctcagcca ccttgtcgct
     3901 gccatcggtt ggcgtatcga gcacgagtat aatttcaatg tcagtaaggg tctgaccgat
     3961 gagcgaatcc aaacagcggg agaagaattt acctgcatta tgaacgggta caacgacact
     4021 aacttttggt ttcattatta ttcaggtttt gccaccgttg caaattgctt ataccggatg
     4081 tcattcagtt ttcccaggaa aagcctgata aaaagtttct ccttcgggct tttacaggaa
     4141 ttaattccct gatgtttcaa cactttatac ccttgttcct gatacattct caggatactt
     4201 ttcgaggtga aaaaacggat atgtgtatca tccagaattc ccccttccgg ccggtagcga
     4261 aattcaccct gaaacagaat ttcggtaatg agattggaga tatatcggaa gttgggaatg
     4321 gaactaacca caacaccctg tggggtaagc aaatttttca catttcggag tacatcccag
     4381 ggtgcataca tgtgctcgag cacatcattg aaaacgatac aatcgaaata atttcgggga
     4441 agctgaccgt aaatttcatt aaaatcgccg acaaaaacct gtttgcactt cttcctggcc
     4501 aattcagctg actgcggatt gatttctata ccccagattt cacggtcttc gcgcgacatc
     4561 atggatgaaa agacaccttc gccgcatcca acttcgagaa tgaattttgc attttccggg
     4621 ataaattcga gcatctcttt cctctggttc tggtaatacc ctattcctgt cttaatttgt
     4681 attgatgatt tatcagacat agttattatt tcgaaatttt atatcagtta ttttttcaat
     4741 gttgccaaca gctactgaat atgccagcca ttgttttcga atgtaatgat gatattttcc
     4801 tttttcaaat acggttccag taacttacca gactccaaat tcagatattc aaaactgtgg
     4861 tttcgggctt ctatggcgtt cttttcctgt ttaaatgtca gttctataaa tgttgctttc
     4921 agcatatctt caactttctc gccatccact atgctttcgg gtgttttcag ccagttacgg
     4981 ttgttgagtt ttggaaaatc tatctttatt tcattccatc ctttgtcgta aaaatggata
     5041 tacgaactgc ataccggtcc gcatatagta tgaatgacag caatcaccac cgacgtatcg
     5101 gatcgttgga aagttttcaa ttcaaaccgc gtattggatg ctgcctgaac acttatatag
     5161 ttgttcaccg tatcgagtgt cagcagtttc acctggtaac cataactgtt tcgaatgctg
     5221 tccctgcgtt cggatttata gtactcggtc agttcgaggc gcattttgct ctcaagtccg
     5281 ggcatcagga tggcaggcat gtccacaaaa tacttatcga tggtctgagc ctgcccctgc
     5341 atagcgggta tcaggcataa aagtgcaaaa aataatttaa ttagtttcat tattggttgg
     5401 ttctgtcttt gttgtatctg ccggtgtttt cgcaggtttt gtcaatacgt tgaaataatg
     5461 tctcaacaat tcatcccagg tattgaatgt ttctttgtac atgacaccca ctccctgaat
     5521 aaagggagca gaccggagtg tatatcgatc gactgtatga ttgtaggcct tgagacgcag
     5581 tttcccattt tcggtaagga tatattctac atctacatcg cctatgattt tattttttgc
     5641 caggtcgtcg ttccggtaac ccaggttgcc gtttacgaga aggcggttgt ttggctgata
     5701 ggatatttca gcttcatatt cctgagctga ctctgagccc agcccggtac ttctgatatt
     5761 ggctccaaac tgaaaagtat ttgtcatttg ggagagccag ttattgatct ggccgaaagc
     5821 cgtattggca aacagcgacc aggtattgtt acccagcgac gaggcaaaag tggctgcgcc
     5881 cttggcataa tcaggggtat agaatttatt gaacagcagt agatacatca tctgtcggtt
     5941 catcatttcc tccgtattta tcaggttgcg aacctgcatt ttaagtgttt cgtcgctggt
     6001 tggaaggtct atatcaaatt tgatatcggg tgtcatcaaa tcacctgtaa gtttcagcac
     6061 ggaattgacc ggaacgctgg ttcgcgatgt ggaactcagg atagcatcgt tcatcagatc
     6121 acgcaacgaa acggtctgtg aataaatggc agtaatattc atctgggcac gaagcatatc
     6181 tcctgtccag gatatgttgc ttccctgatt gattttgaaa tccttgcgta acagattttg
     6241 cagtgtaaag aggtatttcc cttcgtcaat tgtgtaggtt ccgtagagtt tgatatccga
     6301 aaagtcgtcg tactccattc tcaggctgcc gcttccggtt ccagatagca tatcgcccgc
     6361 ttttggatca ataatcagct gaatttcggc atctggtgtt acatccacca tcaggttcat
     6421 tcgaagtctt gttggtttag cttcactgac gacagtatct tttgtcttgg ttttggtagt
     6481 atccttctga ttgacaaagg taataaagtt attttcattg gctatggcag ctccacccac
     6541 cgagataaag actttggttt tgggctgtgt gctggcattc acatcaatat agatatttgc
     6601 catattgccg taaatgttaa cgttacccga agcatatgct ttcccgtaga aaaattcatt
     6661 gtcgcgcggt tgcgtattga gtgccagcat gttgcgggca tttattttaa ggtcatactt
     6721 catattattg aagttaccat cgtgtgtgat tagtccgttg aggtaacccc ggtttttttc
     6781 ttcatcgtac acggtgatat ttttcaatgc tatcgattta cgggtcagat aaactgaatc
     6841 cgtaaaagaa tatgatgttt tcaggtattc gactgagagt ttggcatttt ctgcaaatac
     6901 actcccttca aacccgattg attttgtttt tccaaacata cgaacttttc ctgtacccag
     6961 tccggtaact tctttggaga aggaactcaa ccagcttgta agaaagccaa ggttcagtct
     7021 gtgtgcatcg taaataaaat caattgaatc ttttgcggga atatacactc cgtttgccaa
     7081 agcgacagta tctttcccat ctataaatgt acctgatgcc tgcagttctt tattttcgcg
     7141 atcccaggta gagtagagat aggcattgcc tatcggtgag ttattcagac gggcattttt
     7201 tacctcaata tccgcttcga atacgggttg tttcaggata gaataaactt ctgcttttcc
     7261 ggttgcatta cctttaatag atacattctt tagtttcacc agcgccatca aaaagtcgag
     7321 gttcaagtca ttcatatcta ccgtcaaaat atcggtttct tttcgggaaa ctgtaccatc
     7381 gaggttaata tattgctttc tattcttaat tctgaaatta tctacagtga atgtagtatc
     7441 gggattaata ctaacctgtg aacccaatat atcccaggta gagtcagcga caacaatctg
     7501 cgtgggaaga aatgtaacca gcgcagaagt gagattattt tctttattga attttgtatt
     7561 tgtctgaatt tcacctgcgt agtttatggt gtcgttgttc tgccatccaa attgtgtgta
     7621 aagcaaatcg ttggcagccg cggctttaag actcacgttt gtcaggtttt tctgattctc
     7681 gagccttgcg cttgcctgca gattgagttg ctggtgttca ttattcagca gcagttcaat
     7741 attcctgatc ttttgactgc cgaatgacag gaaaggtgtt cgggcattca gctccacacg
     7801 ttcattgata tcatcgacaa atcctttaac cgtagtaata ccatcaagag tgaagggcag
     7861 gttgagtacc cttgagatca gttttgtatc cgaaagagtc agatcaatgt tcatataatt
     7921 attcgcatta acctgtttcc tttttccggt aatattttta cctgaaaggg ctggaatata
     7981 attttcgaca atttgtgaaa gggtctgggg aattgtgctg tatttgaaat ccccttcaaa
     8041 attaccgtta atgaggtctg atgtgattgt gaattttgaa gattttgcac cgacttcgga
     8101 tacaaactgt aactgatcca tcaccagctg ttcacctgca ttcaaaaagc ggatgttgtt
     8161 cagcatcaga taaccgttga ggttatcggg cgaatttccc accatgtttg tatttccgtc
     8221 aaatgaaagc tgtgaatctt tatattcttt ggtcagatgg agcgcatgaa tatctgcatt
     8281 attcacctga aggtcaaaat taaaaacagg taattttttg gtaaggtcca ccattccatc
     8341 gaaacctgca tcgaggttcg ggtctttcac atccatttta ccttcaaatc ctgtaccgtc
     8401 gtagttgcca tccaggctga tattctgata gttgtattta tttaggtata tttcagcaat
     8461 atcgccgctg atggttccct ggagacttcg gttatgaagt tttgttcctt ttgtcgtgat
     8521 tttgaaagct gtttttccga ataatttcgt attcagaact tgccccagct gaaaattctg
     8581 tgttcggatt gttccgttgt atttcaggtc gctgagttta ttttcaaatt gtaacagaat
     8641 atccgtattg aggcttcctg cgttggtggt aatatttcca taagcaacca ggttgctgaa
     8701 aaatcctgag atattaccgg tgtagcgcac caccccaagt cgcgaaagtt ctttgggaag
     8761 cacaaacggc ttgtggctga gctgcgagat aaaatcctgc gcgtcgcctt tattgacatg
     8821 aaaatcgcgg atattggcat aaataaacgt ttcatccaga ttgggtattc cgttcaggtc
     8881 cagatcggcc ttcagtgtga gtgattttcc atagttcatc tccatttcat gtaaacggaa
     8941 actcgagatt ctgcctgtga gcaatccacg aacagaggca ttttccttca tgtacctgaa
     9001 attgggtgca aaagcagcaa gatccttcat ggctaccttc gaagttttaa ttctggcatt
     9061 ccatttcacc ttattcagca tattccgtag attggcaaca ctatcgtagg tcatcgaaac
     9121 cgaatcgagt aaaagtgaag tatggggcaa tgatacgtta agaaaaggca tatgaaatcc
     9181 ttttttccat ccatgcacca ccgtttcgat attgctgatg gtcagaccgg agcgttctac
     9241 cgcgctgaaa gagcggatgc gcgccgagag tgaatctttt ttcagataat ccacattcag
     9301 caccagattg aggtgtctga aatccattct gttgccatca aaacgatttg catcggaagc
     9361 tgccggatgc tgaaggttgg tcaggcggaa gcgggaatca tttagacgaa tgtttttgaa
     9421 attaaattca accttcgatt cttttttagg cttctgaggc ttactgaaag ctttaatcac
     9481 aaaatccagg ttgcttactc cggcggtatc aataaccagg ttgccataca gcccttcaat
     9541 tttaatttct ttgaaaacat attttccgga aaagaatttc cagaaatcga aaccggcgta
     9601 tccacgttcc acaaatatca gtgtatcgcg ttgctgatct tcaatataga ggctatcgaa
     9661 acgaaaagaa ttaaaatacc ggtagtcaat ctttccgatg ctgacgcgtg tatgcagttt
     9721 ttccgaaagc tggcgtgtaa cctgacctgc caaataattt tgcaccccgt tatttctgaa
     9781 cgcgacaact gccagggcaa gcaagaaaat gatccccagc aaaacatatc cggttatatg
     9841 tacaagtttt tttatatctt tgaaaattat ttctacaaaa gtagaaaaaa aatatcttat
     9901 aaatactgtc tgtaactgta ttagtcagta cttttgcaaa aaaaatacac ctaaagccgg
     9961 tggggatgaa aaggaaaagc gtaaaacatc tgtaaaggta tgcagatatc ttccagcttt
    10021 cgggccgata agccgattcc cggagaagcg gaatgcagta acagaggata aattaattat
    10081 ttctaagttt gaaaatgtca gaaaatataa ccatcattgg aatcgaatca tcgtgcgacg
    10141 atacttcagc cgcagttatc cgcaacggag tgatgctctc gaatgttatt gcgggtcagg
    10201 ctgtgcatac ccgttacgga ggtgttgtcc ccgaactggc ttcacgtgca catcagcaaa
    10261 acatcattcc tgtagtacac gatgcactgg tgcaggcagg catcgacaag aaagaactta
    10321 cggcgatcgc tttcacacgt ggccccggtt tgctgggttc cctactggtg ggcacatcgt
    10381 ttgccaaggg attttcacag gcgctgaaca tcccgctggt ggatgtcaat catctgcaag
    10441 ggcacgtgct cgcgcatttt atcagtcagg gtaaagagga tgaaaaattt ccttcctttc
    10501 cgtttctctg tctgctagtt tcgggtggta attcgcaaat cgttctggtg cgcaactacc
    10561 tcgatatgga agtgatcggt cagacaattg acgatgcagc cggtgaagct ttcgacaagt
    10621 gtgccaaggt gatgggactt ccctatccgg gcggacctca tgtggacaga ttagccaaag
    10681 agggtaattc gaaagccttc aaactcaaca agccccattt gcagggctat gattacagtt
    10741 tcagcggcct gaagacctct tttctttacc tgcttcgcga cgagatccgg aaaaacccca
    10801 actttgtaca ggaaaatatg gccgacctgt gtgccagcct gcaagccacc atcatcgaca
    10861 tactgatgaa gaaactccgc aaagctgccg acgacctcaa tataaagcag gttgcagtcg
    10921 ccgggggtgt ttcggccaat tccggattac gtgatgcttt ccaccagcac gccgagaaat
    10981 acggctggga tatcttcatt ccaccctttg cctttaccac cgacaatgcc gctatgatag
    11041 ccattacggg ttattataaa tatctgaaag gagaattctg cagcattgag gaagttccgt
    11101 atgcccgggt aagtaattta ctggtgtaaa aattatgaaa ctgcagctgc ccgaaaaact
    11161 gaagccctac agcggagtga tatatttcgt catcattcta ctggtatcgc attttttctg
    11221 gaaatacacc gttctgggcg atgaagaagg ttatctggtc agtttcttcg ggatggatat
    11281 cacactgccg ttcgacttta tggctcgtca tactgcccgt gtaagccatt ggctacttca
    11341 gggactagga ttcagcacct ctctgacgga taacaatgtg attcgtcata cagtaagtct
    11401 caacgccgtg catatagtgt ggtcatgtac gggtatcaag caggcatata ttttgttttg
    11461 catactggca ttcagtcgcg gaccgtggca gcataaactc tggtacatac ctctgggact
    11521 gttgtgtgta tatcttttca atattctgcg tatcactttc attaccgcca taatggatgg
    11581 acaccccgaa caattcgaac tgtggcacga acacatcacc aaatatgcat tttatatcat
    11641 gatttttgga atatgggttg tctgggagga aaagatcagg ctcttcggta tccgaagtca
    11701 ggtacaacaa actaatgctt aaagcaacaa attacttaaa aaaaacctgt cccatttttg
    11761 caacggatca ggttttatta tgttgttggt taaaagtttt attctaaaag ttgtaatcaa
    11821 cgtatccttt cttaacgcta aacactttcg gactttcacc ccggagtata cgcatcgcat
    11881 tggaagcaag tgcggccatt tcatcttcac ccggatatac atacactgtt gccagcggtt
    11941 cgatcatggc tttcacataa tctacgatgt aatcgttgta tgccacgccg ccggtcagga
    12001 ttaccccgtc tgttttgcca tgcagcacag caagcatagc tcctatctcc ttgcccaccg
    12061 aataggcaaa agcctgtaac acctttgcca cctgttcgtc gccgctttcg gctttcttta
    12121 ccagttcctg cacattattg cttccgcagt gagcaatcag cccaccctgt ccggccagtt
    12181 tgcgcaggat ttcatgctga gtatattttc ctgtgaagca catccgaacg agcagaccgg
    12241 cgtcaattgt tccggcccgt tcgggcgaca tggctcccca tccgtcgaga ccctgattga
    12301 cttcaatcac acgccctttg cggtgcgcac caatagttac gccaccaccc agatgtgcca
    12361 caactaagtt gagatcttcg tagcgtttac caactataga agcatgcagg cgggcggtag
    12421 ctttatggtt gagagcatga aaaatcgtcc ttcgtggaat ttcgggcata ccgctgattc
    12481 gagcgacatc gtccaactca tctacagcca ccggatcggc aatgtaagct ttacaaccag
    12541 gaattgccga agcaattttt tcggccagaa atgcgccgag attacttgca tgttcgccgc
    12601 gttcgtgctt gcgaagcgtg tcgagcatca ggtcattcac ttcataaaca cctgaatcaa
    12661 caggcggaag catacccccg cgccccataa taactgagaa atccgacata tttaccccgt
    12721 tgtctcttag tgtttgtata ataagctgat acctgaaatc aaactgatcg agcacatgtt
    12781 cgtattgtgt gacttcggtg gtactatgac gtatggtaaa aacggactgt tgctgctcct
    12841 cttcatatac agcaattttg gtagaggtgg aacccggatt aataactaat attttatgca
    12901 taattatttt tttttcgaca aagataaatg aaatagcgga atggcgcgag atgtacggtg
    12961 gtttttttga aaaaaaaaag aggacagaga aaacctctac cctcgtacct gagaaaatat
    13021 gaatagtaat agtctcccac cgagtgaagc tgtcatttca actattcgat tttttaagcc
    13081 attcctccag ttgaacatcc gctgttttcg cgctgcctcc ctgtatggct attcccggca
    13141 gaagaattgc atcgggacaa agtttccgga tatccgtttc gctgtgacca aaaccgccac
    13201 cctcgtgagt acagaatggt atgatcgttt tgccattaaa actggcatac tccagaaaag
    13261 tgcacacagc catggggaaa gtgccccacc agttgggata gcccagataa attacattat
    13321 aattttcaag gttttcgata tattcagcta gtatggggcg cgcgtttgtt cgcaactcat
    13381 ttctggctat gcgtgtagtc tccatataat cccggggata atctactgct gtgcgaattt
    13441 caaacaaatc gcctcctgtc atcttctgta actttctggc aataatttca gtattaccca
    13501 ccagcagatt tactacattt ccattctggt agttcatccc cctgcgcgaa aaataagcaa
    13561 tcagtatttt attatttcta tttgacattc gtgtaatcac ttttttatct gataaaatat
    13621 attattctaa aaacaacaga aaaaagtttt ggtttggtct gtaactgcaa aaaatatgta
    13681 ataaaaccga aaggtgataa gtaaccagct ctcaacactc aaaaaaaatg aaagtagtag
    13741 tacatttata aaatactatt caaaatttta aatttgctgt atgatacctt ctttttgtga
    13801 agactaagtg aaaatatcaa aaaaaaacat ctacaatatg gtatgtttta caaaataaat
    13861 gacattataa ttatctgttt tcagtaaaaa gttcatttat atttcggatt ttttatat
//
LOCUS       DAOPYB010000068        13889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4827_length_13889_cov_10.832948, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000068 DAOPYB010000000
VERSION     DAOPYB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13889
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4827_length_13889_cov_10.832948"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PKH58_06845"
     CDS             <1..482
                     /locus_tag="PKH58_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNX88782.1"
                     /translation="EQCYNFLKPGGRLAIVLPDGILGNPNTEYVRDWILKRFKLLASI
                     DLPVETFLPQVGVQASLLFLQKKTQAERDAAINGEDYEVFMAIAEAVGKDRRGVPVYK
                     RDEDGAELLFDELKQVLLRDSLGNEKIISKKSRVKRLDDDLPAISQAYIQFLKGIKL"
     gene            479..1843
                     /locus_tag="PKH58_06850"
     CDS             479..1843
                     /locus_tag="PKH58_06850"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNX88783.1"
                     /translation="MKIAITKNSWLHGSDLRLDASFHLSEGRQAKNTIQMLSVEKQNL
                     SKISLKIYNGARFKRVYVSDENRGIAFMGSSDMLKSDFYGLKYLSKVYTKNISELLIK
                     KDWILVSCSGTIGNTVYTSLDFDGKSASQHIMRIIPDNSKIKSGYLYAYLSSKYGYSL
                     LTQGTYGAVIQHIEPEHIENIPVPIIPENKQLEIHNLIAESAELRVEANKLLKNAVNY
                     FNQKYSSSKIETTKVFSKKISQINFSWAAYNNNIESDEIMNRFSIEKVAIKDISEDVF
                     VPPMFKHIYLEKNNGNPFLTGSELTQQNPKFYRYLSTRGVKNIIDYKVQKGTLLLYKS
                     GTTDGGILGNVFIVDDLLNGACLSDHVIRINMKEIRMSYWVYAFLKSNAGIHLLKRLA
                     TGSMIPFITPDKIKELHVPKPDDSFDWVANCISEYISKNTLSNIKENQAIQLVENEIE
                     QWQH"
     gene            1831..5895
                     /locus_tag="PKH58_06855"
     CDS             1831..5895
                     /locus_tag="PKH58_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNX88784.1"
                     /translation="MATLIQAKHSKGAVNAGEERLLKFLEVNLPDHYYIVSNVEFANS
                     TPQGQVQYLEYDCLVVTPHALYHIENKDWSGRLEGDDTTWYLNGSEKPNPLKTVRFKT
                     SVLASKLKAHNPSWATAWIASLLTLSHPRQNKKMLWGDCEKASYLLDNKLIDFICNPN
                     AVRKPADSIADIYAQISEFVAGISTPREPKDRKEIVGYDIIETLALDKCYSEYLCKTK
                     GMITANKVRVKEYILDLVGLSPAERDKRALQTKNQQMALAKMQRCPFILNAQFILDEE
                     NNRFYEITDFLDENSLRAELRRKTFTQEEKLSIIFNLIEALRVAHKVDVFHRDLNPEN
                     IYLTSGCAALANFGKAYFADHSEMGYTVAPTLNETNATAYNAPEMLVKDASRASDIYS
                     MGVLIYELFTGKLPFSSPYELNKLGGKLTPELMPTAVKVGTPAWLDELCMNTILWDDT
                     QRWDSIEELDNFLKYQISTSHVASTQQTAQIVYEELKPGLRIGDFTLYNQLGNGGYSK
                     VFKAKHNIQGKDYAIKIFNESISVQTVIDEYNALANLSNPYIVKFITNGTLPNGQFYT
                     LMEFLDGNNLQEYIRGDLKLPLTIIYQLAIEILTALVCLQKQTPNPIYHRDIKPQNIV
                     WDKQERFVLVDFNVATAEGSETNLVGTIPYLAPDLQQGNKVNWDSSADLFALGITLYE
                     LVCHSYPWGGGMKMPRVGIAPEPIEQYNETISDTFAAFILKAINCSKESRFKSAQEML
                     DALTGIGANNLIKKEEITPQGEVILAGKDDITFVKYLNSLYSQSRYGNAGTRAGFNSS
                     AFDDITYTKTKLDTELLSAILKGKYRLVIITGNAGDGKTAFIKQIEKEASNVERFSNR
                     NGAKFQINGRAYQSNYDGSQDEEDRANNEVLSDFFQPFENTSDYSSVNEGRIIAINEG
                     RLVDFLETSDKFRGLSDIIDQYFYNSGHADLPDGLMIINLNLRSITAKSNGCESLFRS
                     QIKLLTKPVLWSKCNTCSHAEHCFIKYNVDTLTDNAAGDEVINRLEWLMRTISYKREL
                     HITMRDLRSFLAYLISRDCNCNDISELYKRFENEPEKYWQYYYFNITSQDAEKSNDRL
                     ITLIREADISQVALPAIDRDLYFGLHAPKDYLNFSDRQVDLLDAFNSQKLLLPAYDLK
                     DRILEQMKLRHRTYVRHQYFEGKFEFKKRLPYQSLEEFHTLLTNTHNNEQDIESAKQS
                     LAQAISISEGCSESGLANNFLLLKSSRINDPISQSFRRFSLSEFELFVNKTEHLIQYI
                     EYESDSLIFRNKEDDFISLTISLDLYEMLYFIKQGFSPSINDMRGRFIELQVFKNLLE
                     NKSYNEVLVTKNNKSFFVISLEQKTNKLTIAPLN"
     gene            5903..7456
                     /locus_tag="PKH58_06860"
     CDS             5903..7456
                     /locus_tag="PKH58_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88785.1"
                     /translation="MAIRLDKNEAIFRNELILAADAKSVNLDNTLTNLFMLIRNNGAR
                     INMRVKREHTFQSLIEYMENLETHGNMVGLSEHKEAAEDWLRCNLVNLVFRGNIVKEK
                     ISSLRPVHLESFRIRNIAQTRDYNSSDQVFLMLRMKPEIMESLKEYLSKGWDNVTKNI
                     SKNQKLDVDSAGILQLIKNIQIDPKSSAQLKNVRPLLEEQANLFCDDILRLLVYKDSI
                     PRNVFIEYLRTLIGFHLSLYTQKSIYLLPRMVKEGTRNVVDDFSLIVDVTDNFESRVS
                     RFACEDMERTINGLYEFFKASFEINAVQKRYETTMPAVDSVDQWLSILKNRPDNFDIY
                     YENKFNIILDKFKPEEEEDKKNLLDCVMYEESFFDKYVMTLLKAKGAYQYRYYQQFLD
                     AISMKNTDYGFLADGRSRKHPRRTVIGSRLLETLVQILVLEPALGGFSSRSLSIDELT
                     AKLRSRYGIVINGLNESRFNDADLETHLAFKENVDAFKTKLRQIGFYTDLSDAYVLQK
                     IRPRYKIKA"
     gene            7456..12894
                     /locus_tag="PKH58_06865"
     CDS             7456..12894
                     /locus_tag="PKH58_06865"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNX88786.1"
                     /translation="MEISELYYQLLLSIIVEEYKAEFTSAKPGHCMKITGLSMRELNP
                     LCSQIRALEGELQTYILSDDLQGDEYISANKLIELRNNDGFPLLVLIPSNSRTSTEDS
                     YGNATFKNLAIEHLDTKLLSVLKTEIPTEIKPIVNEIFDYLKIQNLRQAQYLQYLLFL
                     KLNNWKKEAIGDGLATFAILPDDKISIDIKQLRQRLLYNLRCVDILSDFSKPIPDRIK
                     DLPVEPNSIQNRITYLLKSENQIKSKGDICQAVLTKYPDLNFSNWSVPDFDTPHDLHV
                     FVDKISSNEIRTQDGDLTLTIPRDKTVKVKIRITTKPAPKDFKELKSFRIVLMAIDGW
                     YPVFDVKKTKVTENSRNYRDITMEFSETMVEEGSYFFRIFAEDENGGILNTLDNYALP
                     EFEDLWNSQKKQTPDLSHEDFESSNLAKRTNDSEDFYINFDELSGEEVEPHRKDKLDN
                     VLQAYFRFRIEQLRNNNELSMPEPIDNTAVWLKGTDLGLVNTFHIKYATNHNYQINIS
                     GKLRKLQSYFLKHAGEIGNIDVRLSNNPTDNDFQSLRFNKFLDDEIIDKNLISVRKEL
                     FELIAQSAPDNTGVIETFDIFNHIDLIRKYIVLYDKWTKSIMKAVDADELQTTDFHKT
                     LSGLQNLEIISVKTEMPDGQTVYLKLVSPLHPLRLAWFVNLFDLYSNWEEETRTTFPQ
                     YKTVWYKNLENLFLGDLLPEIALPVLTDSSVSEYFQYVGELSYGWGLYTKSLQKSNDA
                     FSSISRQIKIYLASLLNISNEYRIDTDVNQTLVSRHIQNYLTQHPYINKLVVNLFNAG
                     DAYVFANAMVDIERLPEFKSIKYEVRLFAHEKIIVPGEAFRNLTNPESNISEDAEAFS
                     QASGNRLFPKLRFSINSVADFIQNPNDYFSHIAFMISPFPVKTTLARPEESKQSFYFN
                     GLINQSVISVVQKSDSIVWNKYFSENPVVNSVNDFCNVGVSLFSNFQVFVANSLSTNR
                     EKSIPATKLELNESDKVLLSFIHDTSDWVITFDKNLGPEVYDLPGAEGEIPFLLDYVP
                     GQEISGISSYLTTRPTSEIIGLIGPHFEEFGIDLRNDSAKLKILLEDIRSISSSLIMQ
                     LNSTNNKAFEVLGMAFTKRVLEKKNILEDSFLIPIDLHKELFTNTPNENKERADILLV
                     KINPQTMDICFTVIEVKCRTYLSDNDRIELMAKMQNQIDNTIESLSFHFDRSLSPNND
                     RLDRELKNLQLKSLLEFYINRAARYNHLSQGVYDAYLEYLDNLQYGYNLKFKKLGIIF
                     DFSAVQRHKKEVLDNESTFFTFGNNLIGDILDPDSDLNTRRLEHVKDEEQLIAFFGEQ
                     VKLPLKPINTNISAVSEPLIEFYSKTKIDESREKEDKESNFSEDRNLEEDNTPEVETS
                     LETNESDLPEKEEDNKEVFVSDNSVIDINNEVTPDYDIIIGKSSSSEQYGVLGKVVAN
                     GKSVALDLTETNTISLFGVQGGGKSYTIGSITEMVIKQFSNINKLPAPLAGVIFHYSE
                     SMDYEPEFTSMINPNNNISELEKLKREYNANPSSLSDVVILTPRAKVNERREQFPSID
                     VYPINFNSNELSVQDWMFLLGAIGNDSTYIRQLKAIMRNIRDNISLISLRNNVAESVL
                     LSNTQRKLAEQRLQFADEYIDDNSQIRDLLRPGRLIIVDLRDEFIEKDEALGLFVVML
                     NIFSGVKTFNDIPFNKFIVFDEAHKYMDNKDLTNSIVTSIREMRHKGVSIMIASQDPI
                     SLPNEIIELSSIVLLHKFNSPQWVKHIQRSITQLSHLTPIELSILSPGEGYLWATKST
                     DKGITNRPVKISTRPRVTKHGGETKKAI"
     gene            12897..13583
                     /locus_tag="PKH58_06870"
     CDS             12897..13583
                     /locus_tag="PKH58_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNX88787.1"
                     /translation="MKIFSYTDKGLRETNEDYIKSEILSDSISLHIVADGMGGYDHGE
                     EASKTATEAIFQFFAEKKIEGDIPELIKNAVEFANSQIFTIRNHYHTKLGTTIAGVVI
                     IGTIAYTFWLGDVQIQHFRDNKEIFISESHTLINDMKQNGSVSSKEIERFQHVVTKSI
                     SGNKIIDTISIQKLKILPQDVLIISTDGFYNFVKNDNVSLESSINIEQFIQNNCSIFS
                     DNASFIKILI"
BASE COUNT         4671 a         2493 c         2685 g         4040 t
ORIGIN      
        1 ttgagcaatg ttacaacttc ctgaaaccgg gtggtcgctt ggccattgtg ttgcccgatg
       61 gtattttggg taacccgaat acggaatatg ttcgtgactg gattctgaaa cgtttcaaac
      121 tcctggcttc tatcgatctc ccggtagaaa cattcctccc tcaggtaggt gtgcaggctt
      181 ccttattgtt cctgcaaaag aaaacccagg ccgaaaggga tgctgcaatt aacggcgaag
      241 actacgaggt ttttatggcg atagcagaag ctgttgggaa agatcggcgt ggtgtccctg
      301 tatataaaag agatgaagat ggtgcagaat tactgtttga tgagttgaag caagtactat
      361 tgagagacag tcttgggaac gaaaaaatca ttagtaaaaa gagtcgagta aaaagattag
      421 atgatgattt accggctatc agtcaagcat acattcaatt cttaaaagga ataaaactat
      481 gaaaattgca ataacaaaga attcatggtt gcatggtagt gacttaagat tggatgcatc
      541 atttcatttg agcgaaggta gacaagcaaa gaataccatt cagatgctat ctgtggaaaa
      601 acaaaactta tctaaaatat cgctgaaaat atataatgga gcaaggttta aaagagtata
      661 cgtttctgat gaaaacagag gaattgcatt catgggaagc tctgatatgc tcaaatcaga
      721 cttttatggt ttaaaatatt tgtctaaagt ctacaccaaa aatatttccg aacttttaat
      781 caaaaaagat tggatattgg tgtcctgttc aggaacaata ggaaatacag tttacacaag
      841 tttggatttt gatggaaaat cagcctctca gcatataatg agaataatac cagataattc
      901 taagattaaa tctggttatc tatacgcata cttatcttca aagtatggtt attccctgtt
      961 gactcaaggc acatatggag ccgtaattca acatattgaa ccggaacata tcgaaaatat
     1021 tccggtacca attatccctg aaaacaaaca attagaaatt cataatttga ttgctgaatc
     1081 ggcagaattg agagtggagg caaataagct gctgaaaaat gctgtgaatt attttaatca
     1141 gaaatatagt tctagtaaaa ttgaaaccac aaaagttttt tctaaaaaga tctcacagat
     1201 taattttagt tgggcagcct ataataacaa tattgaatca gacgaaataa tgaatagatt
     1261 tagtattgag aaggtcgcta ttaaggatat atctgaagat gtttttgtgc ctccaatgtt
     1321 caaacacatt tacttagaaa aaaataatgg aaatcctttt cttacaggaa gtgaacttac
     1381 acaacaaaac cccaagtttt ataggtactt atctacaaga ggagttaaaa atatcataga
     1441 ctacaaggtt caaaaaggca ctttgctttt gtataaatca ggtacaactg atggaggaat
     1501 tcttggaaat gtttttattg tagacgattt gttgaatggt gcttgtttat cggaccatgt
     1561 tattcggatc aatatgaaag aaattcgaat gtcgtattgg gtgtatgctt ttctgaaatc
     1621 aaatgccggc attcatttac ttaaaagatt agctactggt tcaatgattc cttttattac
     1681 tcctgataaa ataaaagaat tacatgtgcc aaaaccagat gatagttttg attgggtcgc
     1741 caattgtatt tctgaatata tatcaaaaaa cactttatcg aatatcaaag aaaaccaagc
     1801 catacaactt gtagaaaacg aaatagaaca atggcaacac tgatacaagc aaaacacagc
     1861 aaaggagcgg taaatgcagg agaagagcgt ttactgaaat ttttggaagt aaatcttccg
     1921 gatcactact acattgtttc aaatgtggaa tttgccaatt ccactccaca aggtcaggta
     1981 caatacctcg aatacgattg tttggtagta actcctcatg ccttgtatca catcgaaaac
     2041 aaagactgga gtggtcgcct cgaaggcgac gacactacct ggtacctgaa cggaagtgaa
     2101 aagcccaatc ccttgaaaac ggttcgcttt aaaacttccg tgttggccag taaactgaaa
     2161 gcccacaacc catcgtgggc taccgcatgg attgcttcac tcctcacttt atctcacccc
     2221 cggcaaaaca agaaaatgct gtggggcgat tgtgagaaag cctcttactt attggacaat
     2281 aaattgattg attttatttg caatcccaat gcggtcagaa aacctgccga tagtattgcc
     2341 gatatttatg cgcagataag tgaatttgta gcgggcatat cgactccccg cgaacccaaa
     2401 gaccgtaaag aaattgtggg ttacgatatt atcgaaaccc tggccctgga taaatgctac
     2461 tccgaatacc tttgcaaaac caaaggcatg attactgcca acaaagtccg ggtaaaagaa
     2521 tacatactcg atttagtggg gttaagtcct gccgagcgcg acaaacgggc actgcaaacc
     2581 aaaaaccagc aaatggcact tgccaaaatg caacgctgcc cctttatcct gaatgcgcaa
     2641 tttatcttgg acgaagaaaa caatcggttc tatgaaatca cagactttct cgatgaaaac
     2701 tccttacgcg cagaactccg ccgtaaaaca tttacccagg aagagaagct gagtatcata
     2761 ttcaacctga tagaagcctt gcgcgtagct cataaagtgg atgtatttca cagggatttg
     2821 aatcctgaaa atatttacct cacaagtggt tgcgcggcat tggcaaactt tggcaaagcc
     2881 tattttgccg accacagcga aatgggctac acggttgcac ctacgctcaa tgagaccaat
     2941 gccaccgcct acaatgcacc ggaaatgctg gttaaagatg cctcccgtgc aagcgatatt
     3001 tattccatgg gggtactgat ctacgaacta ttcacgggca aattaccatt ttcgtctcca
     3061 tacgaactca acaaattagg tggaaaattg acacctgaac taatgcctac cgcagtaaaa
     3121 gtgggaacac cggcatggtt ggacgaattg tgcatgaata ccattctttg ggacgataca
     3181 caacgctggg acagcattga agaactggac aactttttga aataccagat ttctacttcg
     3241 catgtggcct ccacgcagca aactgcacag attgtgtacg aggaactaaa acccggttta
     3301 cgcattggtg attttacact ttacaatcaa ttaggcaatg gaggttattc gaaggtattt
     3361 aaagcaaaac acaatattca aggtaaggat tatgcgataa agattttcaa cgaaagtata
     3421 agcgtacaga ctgttatcga cgaatacaat gccctggcta atttgtccaa tccttatatc
     3481 gtaaaattca ttaccaacgg cactcttccc aatggtcagt tttacacact aatggagttt
     3541 ctggatggaa ataacttaca ggaatatatt aggggagatt taaagctacc gcttactatc
     3601 atttaccaac tggccataga aatactcacg gctttagtct gtttacaaaa acagactcca
     3661 aaccctattt atcacaggga tattaaacct cagaacattg tctgggacaa acaggaacgc
     3721 tttgtattgg ttgacttcaa tgtggctaca gccgaaggaa gtgaaacaaa ccttgttggt
     3781 accattcctt atttagcccc tgacctccaa cagggtaaca aagtaaattg ggacagctca
     3841 gccgacttat ttgcgctggg tattaccttg tacgaattgg tgtgtcattc ctacccctgg
     3901 ggtggtggta tgaaaatgcc tcgggtggga atagctcctg aacccattga acaatacaac
     3961 gaaacgattt ccgatacttt tgctgcattc attctgaaag ctatcaattg tagcaaagag
     4021 agccgattca aatcggcaca ggaaatgttg gatgccctta cgggtattgg tgctaataat
     4081 ctgatcaaaa aggaagaaat tacaccacag ggtgaagtta ttttggcagg taaagacgat
     4141 atcacatttg tgaaatattt gaattcactc tatagtcaaa gccgttacgg aaatgcgggt
     4201 acacgggccg gattcaattc cagtgctttt gacgacatta cctatactaa gaccaaattg
     4261 gataccgaat tgctgagtgc aatactaaaa gggaaatatc gattagtcat tattaccggg
     4321 aatgccggtg atgggaaaac ggcttttatc aaacaaattg aaaaagaagc ttccaatgtg
     4381 gaacgatttt caaaccgcaa tggtgcaaag tttcaaatca atggccgggc ctatcaaagt
     4441 aattacgatg gttcgcagga tgaagaagat agagcaaaca acgaggtttt atccgatttc
     4501 tttcaaccct ttgaaaacac atcagattat agcagcgtaa acgaaggaag aattatcgcg
     4561 atcaacgaag gtcgtttggt agactttctg gaaacttccg ataagttccg ggggttgtcc
     4621 gatatcattg atcaatactt ttacaacagc ggtcatgccg acttaccgga tggtttgatg
     4681 attattaatt tgaacttacg ttcaataacc gcgaaaagca acggatgcga aagtttgttc
     4741 cgttcgcaaa tcaaattact cacaaagcct gtgctttgga gtaaatgcaa tacctgttca
     4801 catgcagagc attgtttcat aaaatacaat gtcgatactt taacggataa tgctgccggc
     4861 gatgaagtga ttaatcgttt agagtggctg atgcgcacta tcagttataa aagggagctg
     4921 catatcacca tgcgcgattt acgctcattt ctggcgtatc tgattagtcg ggattgtaat
     4981 tgcaatgata tttcggaatt atacaaaaga ttcgaaaacg aacccgaaaa atactggcaa
     5041 tattactatt tcaatattac ctctcaggat gccgaaaaat ccaacgacag attaataaca
     5101 ctgatcagag aggctgatat ttcacaggta gccttaccag ctattgacag ggatttatat
     5161 tttgggcttc atgcgccaaa agactatttg aatttctcag atcgccaggt tgatttattg
     5221 gatgccttta attctcaaaa gttgttgcta ccggcctatg atttaaaaga tagaatactg
     5281 gaacaaatga aacttcgtca ccgcacatac gtgcgtcacc agtatttcga aggtaaattt
     5341 gaatttaaaa agcgcttacc ttatcaatcc ttggaagagt tccataccct gttaaccaat
     5401 acgcataata atgaacagga cattgagagt gcaaaacaaa gtttagctca ggcaatttct
     5461 atcagtgagg gttgttccga aagtggattg gcaaacaact tcctgctgtt aaagtcttct
     5521 cgtataaatg acccgattag tcaaagcttc agaagattct ctttaagcga gtttgagcta
     5581 tttgtaaata aaacagagca cctcattcaa tacattgaat acgaaagcga tagtttgata
     5641 tttagaaaca aggaagatga ctttatttcc ctgacaatct cccttgattt gtacgagatg
     5701 ttgtatttta tcaaacaggg ttttagccct tctatcaacg atatgagagg aagatttatt
     5761 gaattacagg tattcaaaaa cctgctggaa aataaatcct acaacgaagt gctggtcaca
     5821 aagaataaca aatcattctt tgtgatttca ctcgaacaaa aaacaaataa actaacaatt
     5881 gctccattaa attgatacaa ccatggcaat acgtttagat aaaaacgaag ctattttccg
     5941 taatgaactg atactggctg ccgacgctaa atcggtgaat ctggacaata cgcttacaaa
     6001 cctgtttatg cttatccgga acaatggagc aaggattaac atgagagtga aaagagaaca
     6061 tacttttcaa tcgttgatcg aatatatgga aaacctcgaa acccatggaa atatggttgg
     6121 actttctgaa cataaagaag ctgcggaaga ctggctacgt tgcaatttgg ttaatctggt
     6181 atttagagga aatattgtaa aagaaaaaat ctcttcgctt cgtcccgttc acctcgaaag
     6241 ctttcgtatt cgtaacattg cccagacccg tgattacaat tcctccgatc aggtattttt
     6301 gatgcttcgg atgaaaccgg aaattatgga aagcctgaaa gaataccttt ccaaaggttg
     6361 ggataatgtc acaaaaaaca tatccaaaaa tcaaaaattg gatgtcgata gcgctggtat
     6421 tcttcaactg ataaagaata ttcagattga tccgaaatca agtgctcaat tgaaaaatgt
     6481 gcgtcctctg ctcgaagaac aggcaaatct tttttgtgat gatattctcc gtttgctggt
     6541 ttataaagac tcaatacccc ggaatgtatt cattgaatac ctgcggacct tgattggatt
     6601 tcatttatcc ctttacactc aaaaatcaat atatctctta cccagaatgg taaaagaagg
     6661 tacacgaaat gtggtagatg atttttctct gattgtagat gtgactgata actttgaaag
     6721 tcgtgtttct cggtttgcct gcgaagatat ggaaagaaca atcaatgggc tgtatgaatt
     6781 tttcaaagct tctttcgaga tcaatgctgt tcagaaaaga tatgaaacca ctatgcctgc
     6841 tgtggattct gttgatcagt ggctgtcaat tttaaagaac agaccggata acttcgatat
     6901 atattacgaa aacaaattca atattattct cgataaattt aaaccggaag aggaagaaga
     6961 taaaaagaac ttattggatt gtgtgatgta tgaggagagc ttcttcgata agtatgtgat
     7021 gactttgcta aaagcaaaag gagcatatca gtatcggtat tatcagcagt ttctggatgc
     7081 aatttccatg aaaaataccg actatggatt cttagcggat ggtagaagcc gcaaacaccc
     7141 tcgtagaact gttatcggtt cccgtcttct tgaaacctta gtgcaaattc tggttttgga
     7201 acctgcactg ggtggttttt cctcgcgttc attatcaatt gatgagttga cagcgaaact
     7261 tcgttccaga tatggaattg taataaatgg gctgaatgaa tcaaggttca acgatgcaga
     7321 tttagaaact caccttgcat ttaaggaaaa cgtagatgca ttcaaaacta aattacgtca
     7381 aattggcttc tataccgatt taagtgacgc ttatgttcta caaaaaatca gaccacgtta
     7441 taaaatcaaa gcataatgga aatatcagaa ctttattatc agctacttct ctcaattata
     7501 gttgaagaat acaaagcaga attcacaagt gcgaaacccg gtcattgcat gaaaataacg
     7561 ggtctctcta tgcgtgaact aaatccttta tgtagtcaaa ttcgtgcctt ggaaggtgag
     7621 cttcaaacct atattttgtc tgacgatctg cagggggatg aatacattag tgccaacaaa
     7681 ttaattgaat tacgaaataa cgacgggttt ccattattgg tactgattcc gtcgaacagt
     7741 cgaacttcta ccgaagactc ctatgggaat gcgactttca agaatcttgc aatagagcac
     7801 ctggatacaa agctattaag tgttttaaaa actgaaattc cgactgagat aaaaccaatc
     7861 gtaaatgaaa ttttcgatta tctgaaaatt cagaatctca gacaggctca atatctgcaa
     7921 tatctgttat tcctgaaact aaacaattgg aaaaaagaag ctatcgggga tggattagcc
     7981 acctttgcga tcttacccga cgacaaaatc agtatagata tcaaacagct tagacaacga
     8041 ttactttata atctaagatg tgtagatata ttaagtgact tctctaagcc gattcctgac
     8101 aggataaaag atttgccggt tgaaccgaat tcaattcaaa atcgcataac atatttgctg
     8161 aaatctgaaa atcaaataaa aagtaaagga gatatttgcc aggctgtatt gacaaaatat
     8221 cctgatttaa acttcagtaa ctggtcagtt cctgacttcg acacgccaca cgacctgcat
     8281 gtttttgttg ataaaataag ctcgaatgaa atcagaaccc aagatggtga tctgaccttg
     8341 accattcctc gcgacaaaac agtaaaagtg aaaatccgga ttactactaa accggctcca
     8401 aaagatttca aagagctaaa gtcatttaga attgtattaa tggctattga tggttggtat
     8461 ccagtatttg atgtaaagaa aacgaaagtt acagaaaatt cccgtaatta cagggatatt
     8521 accatggagt tttctgaaac aatggtggaa gaaggctctt actttttccg tatctttgct
     8581 gaagatgaaa atggtggtat tttaaacact ctggataatt atgccctccc tgaatttgag
     8641 gatttatgga attcacaaaa gaaacaaacc ccagatcttt ctcatgagga ttttgaaagt
     8701 tcaaatctgg ccaaaagaac aaatgactcg gaggatttct atataaattt tgatgaactt
     8761 tcgggcgagg aagtagaacc gcaccgaaaa gacaagcttg ataatgtttt acaggcttat
     8821 ttcagatttc ggattgagca acttcgaaac aacaatgaat tatcaatgcc ggaaccgatc
     8881 gacaacacgg cagtatggct caaagggact gatttaggac ttgtaaacac ctttcatatt
     8941 aaatatgcga caaatcacaa ttaccagatt aatatatctg gtaagcttcg aaaactacag
     9001 agctactttc taaaacatgc gggtgaaatt ggaaatattg atgtaagact cagtaacaat
     9061 cctaccgaca acgattttca atcattgagg ttcaataaat tcttggatga tgaaataatt
     9121 gataagaatt taatttcagt tagaaaagaa ttgtttgaat taatcgcgca atctgctcca
     9181 gataacacag gtgtaattga aaccttcgat atatttaatc atattgatct gattcgaaaa
     9241 tacatagtcc tgtatgataa atggacaaaa tccataatga aggcagtgga tgcagatgaa
     9301 ttgcaaacga ctgattttca taaaacatta tctggcttac aaaatcttga aatcatttca
     9361 gtgaaaacag aaatgcccga tggacagaca gtgtatctga aattggtttc cccccttcat
     9421 cccctacgct tggcgtggtt tgtaaatctt ttcgacttgt attcaaactg ggaagaagaa
     9481 actagaacta catttcctca atacaaaaca gtttggtata agaaccttga aaacttattt
     9541 ttaggtgact tattacctga aattgcgctt cctgttttga cggattccag tgtatctgaa
     9601 tactttcagt atgtaggtga attatcttat ggctggggcc tttacacaaa atcgctacaa
     9661 aaaagtaatg atgctttttc ttcaatctcc cgccaaataa aaatttattt ggcgtcattg
     9721 ctcaacatta gcaatgaata tcgcattgat accgatgtaa atcaaacatt ggtgagtcgg
     9781 cacattcaga attatcttac tcaacaccct tatatcaata aattggttgt caatttattc
     9841 aatgccggtg atgcatatgt ttttgcaaat gccatggttg atattgaacg attaccggaa
     9901 ttcaaatcta ttaagtatga ggtaaggtta tttgcccatg aaaaaatcat tgttcccgga
     9961 gaagcttttc gcaatttgac aaatccggaa agtaatatct ctgaagatgc tgaagctttt
    10021 tcgcaagctt ctggtaatcg tttatttcct aaattgcgtt tctctatcaa tagtgtagct
    10081 gattttattc aaaatcccaa cgattatttt tcgcatattg cttttatgat tagtccattt
    10141 ccagttaaaa caaccttagc ccgcccggaa gaaagcaaac aatcatttta tttcaatgga
    10201 ttgattaatc aaagtgtgat aagtgttgtt caaaaatcag attctattgt ttggaataaa
    10261 tacttctcgg aaaatcctgt tgtgaatagt gtaaatgatt tttgtaacgt aggggtttca
    10321 ttattctcaa attttcaggt atttgtggca aactctttat ctactaacag ggagaaatcc
    10381 ataccggcta caaaattgga attgaatgag tctgataaag tgctattgtc ttttatccac
    10441 gacacttccg attgggtaat taccttcgat aaaaatttag gccccgaagt atatgattta
    10501 ccgggggctg aaggtgaaat tccatttctg ttggattatg ttccggggca ggaaatctcg
    10561 ggtatttcct cctatctgac tacccgtcct acttctgaaa ttattggact gattggccct
    10621 cattttgaag agttcggtat tgatcttaga aatgattcgg ccaaacttaa aatattgttg
    10681 gaagatatcc gctcaataag tagctcatta atcatgcagc tcaactccac caacaacaaa
    10741 gcttttgagg tgttggggat ggcatttacc aagcgggtat tagaaaagaa gaatatcctg
    10801 gaagattctt ttctcattcc aattgatctt cataaagagt tgtttacaaa cactcctaat
    10861 gaaaataagg aaagagcaga tatcttatta gtaaaaataa atcctcaaac gatggatata
    10921 tgcttcactg tgatcgaagt gaaatgtcgc acctatcttt cagacaatga tcggatcgag
    10981 ttgatggcga aaatgcaaaa tcaaattgat aatacaattg aatcattgag ctttcatttt
    11041 gataggtctc tttctccaaa taatgataga ttagatcgcg agttgaaaaa cctgcaatta
    11101 aaatccctac tcgaatttta cattaatcgg gctgcaagat ataatcattt atcccagggt
    11161 gtatatgatg catatttgga gtatctggat aatcttcaat atggttataa cctgaagttt
    11221 aaaaaactgg ggataatctt cgacttctct gcagttcaac gtcataaaaa agaagtattg
    11281 gacaatgaat ctacgttctt tacctttggt aataacttaa ttggagatat tcttgatcca
    11341 gattcagact taaatacacg tagactggaa catgtgaagg atgaagaaca attgattgct
    11401 ttctttggtg aacaggtcaa attgccatta aaacctataa atacaaatat ttcagcagtc
    11461 tctgaaccac taatagaatt ttattcaaaa accaaaattg acgagagtcg tgaaaaagag
    11521 gataaagaat cgaatttttc tgaagatcgt aatttggagg aagataatac acctgaggtt
    11581 gaaacaagtc ttgaaacgaa tgagtcggac ttacccgaaa aggaagaaga taataaagag
    11641 gtatttgtgt ctgataattc tgtaattgat atcaataacg aagtaactcc ggactacgat
    11701 attatcatag gaaaatcctc atcgtctgag caatacggtg tactcggtaa agtcgttgcc
    11761 aatggaaaat cagttgcact tgatttgact gaaaccaata caattagcct cttcggtgta
    11821 caaggcggtg gtaagagtta tacgattggt agtatcactg aaatggtgat taaacagttt
    11881 agcaatatca ataagttacc agctccttta gcgggggtga tttttcatta cagcgagagt
    11941 atggattatg aaccggaatt cacgtccatg attaatccaa ataataatat atcagaattg
    12001 gaaaaattga aacgagagta taatgcaaat ccgtccagtt tatccgatgt tgtaattctt
    12061 acaccacggg caaaggtaaa tgagagaaga gaacaattcc cttcaattga tgtctaccca
    12121 atcaacttta attctaatga actgagtgta caggattgga tgtttttatt aggtgctatc
    12181 ggcaacgatt caacgtacat tcgtcaattg aaagcaatca tgagaaatat ccgggataat
    12241 attagtttga ttagcttacg taataatgtt gcagaatccg ttctattaag taatacccaa
    12301 cgtaaattgg ctgaacaacg actccaattt gcagatgaat acattgatga caactcgcaa
    12361 atcagggatt tgcttcgccc gggaagatta atcattgttg atttacggga tgagtttatt
    12421 gaaaaggatg aagcacttgg tctgtttgta gtaatgctga acattttctc tggagtgaaa
    12481 acattcaatg acattccatt taataaattc attgtatttg acgaagcaca caaatacatg
    12541 gacaacaaag atttgaccaa cagcattgta acctcaatca gagaaatgcg gcacaaagga
    12601 gtatccatta tgatagcgag tcaggatccg ataagtctgc caaatgagat aattgaattg
    12661 agttccattg tgttattgca taaattcaat agtccacaat gggtaaaaca tattcagaga
    12721 tcgataacgc agctttccca tttaacccca attgaattat caatcttgtc cccaggcgaa
    12781 ggatatttat gggctacaaa atcaacagat aaaggcataa cgaatagacc tgtgaagatt
    12841 tccacacgcc cgcgtgtgac aaaacacggt ggtgaaacaa agaaagcaat ttgacaatga
    12901 aaatcttttc atataccgat aaaggactta gagaaactaa cgaagactat ataaaaagcg
    12961 aaatactatc agatagtatt tcgctccata tagttgctga cggcatgggg gggtacgacc
    13021 acggagaaga ggcttccaaa acagctacag aagctatttt tcaattcttt gcagaaaaaa
    13081 aaattgaagg agatatacca gaactaatta aaaatgcggt tgaatttgcc aatagtcaaa
    13141 ttttcacaat acgaaatcat tatcatacta aattaggaac aaccattgct ggagtagtca
    13201 taattggcac tattgcttat actttttggt taggagatgt acaaatacaa catttcagag
    13261 ataacaaaga gattttcatt tctgaaagtc atacccttat taatgatatg aaacaaaacg
    13321 gttcagtatc tagtaaagaa atcgaacgtt tccaacatgt ggtcacaaaa tcaatttctg
    13381 gaaataaaat tatagataca atttctattc aaaaattaaa aattttgcca caagatgtct
    13441 taataatttc aactgatggc ttttataatt ttgtaaaaaa tgataatgtt tccctagaat
    13501 cttcaataaa tatagaacaa tttattcaga ataattgttc tatattttct gataatgctt
    13561 cttttataaa gatcttaatt taaaaatttt tttatggctt cattccatgt aaaaaaatac
    13621 taattatatc attttggaat cgtaaataca tagttcataa tctcttttcc agtggtttta
    13681 caagtctctt ttcttaaaga aataaaaaat tgtttatttg tattttaaaa ttgtaattta
    13741 ttacagatag ctgtttagtt cttgcattag ggcgaaggcc aagtaccccg caagcaggga
    13801 cgcctagtgg tgcacgagac cggcagagcg accctgggag ctactgccgg gccgatgcaa
    13861 cacttggcgg aactgcgagg agtacaggc
//
LOCUS       DAOPYB010000069        13787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4881_length_13787_cov_9.365278, whole genome shotgun sequence.
ACCESSION   DAOPYB010000069 DAOPYB010000000
VERSION     DAOPYB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4881_length_13787_cov_9.365278"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..858
                     /locus_tag="PKH58_06875"
     CDS             208..858
                     /locus_tag="PKH58_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNX88788.1"
                     /translation="MKRTILFFTFTALVFSLSAQNSVVIKLNEHTKYRGYNTMEAFQK
                     RRSVNIYETRDLSLQDLSDLLWAANGINRPENGKKTAPSAMNSQDVDIYVSRANGTYL
                     YNAITNDLVLVTTEDLRPLMDGNRPLGTPVILLLVSDMSRYKNYSPGNPDQNRHLFEV
                     GAIDAGIVSQNISLFCAANDLGTRPRASMNQVALRKALNLKESQVMWLNHPVGYPK"
     gene            877..1749
                     /locus_tag="PKH58_06880"
     CDS             877..1749
                     /locus_tag="PKH58_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNX88789.1"
                     /translation="MNKRKCTQIVRGQQAVDGAGVHLQRVLGLRTVKDFDPFLMLDGF
                     DSSNPADYIKGFPWHPHRGIETVTYLISGEIEHGDSLGNRGTIRDLECQWMTAGSGII
                     HQEMPVASERMLGCQLWVNLPAKDKMTDPAYRDITQKDVATVEDENAAIRVLSGSYKN
                     HTAAVSGNYVKVTYLDVQLKPGTKWTYNETPNDETLFIYLIDGTLAPAGEGAAFEEKA
                     CAILFTASNELSSDFEELQVKAGSNGARFLLLAARPLKEPVAWGGPIVMNTREELNQA
                     FDELETNNFIKHKM"
     gene            2019..2780
                     /locus_tag="PKH58_06885"
     CDS             2019..2780
                     /locus_tag="PKH58_06885"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNX88790.1"
                     /translation="MSLKKTVITFLLLAGIQLAGAQIRPNETLVYNGSYQLSGMMTSL
                     AQITMKTEPIKTSSKSYLRLCVQVATYTKWDSFFKIRDLYESYVHPYSLKPTLFKRNV
                     YEGGYTKQEKYTFNAAGSQVSAYSKRRDKPAETENVNISANTFDAVTLMYKLRTVDLS
                     KMAKGQSLRFSLIFDKKEHFIWIKMMGKETVSAGNLGKKECFKLSISAKTDKLRGVDK
                     NLVWLSTDAKRIPCMMKFSIPVGIGQVTLSKATGI"
     gene            2791..3102
                     /locus_tag="PKH58_06890"
     CDS             2791..3102
                     /locus_tag="PKH58_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88791.1"
                     /translation="MRKLFNILAVVFAALGLVFAILPMGSIAFLPIILAIILALLAMW
                     KSNDTQKKFPKWVLIISMAILLFVTVKVVFVKDKVVVDEQFMQENVDSKSEAQKELEG
                     L"
     gene            3336..3497
                     /locus_tag="PKH58_06895"
     CDS             3336..3497
                     /locus_tag="PKH58_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88792.1"
                     /translation="MIISLNNEKKPAKAVYYGNEGHTIHTRSNVPIKRLFIDNDKNAE
                     APFFRLVYK"
     gene            3779..4951
                     /locus_tag="PKH58_06900"
     CDS             3779..4951
                     /locus_tag="PKH58_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88793.1"
                     /translation="MRFFVSIILLFCAALALAQSGPISHSGELIAQKRYKSAYQVLDS
                     ADTVNQNPEIVIAKTDLLLNYNIGVNNHRVFALNDSAPSQSVIQNRPIYVNFPADSIL
                     NELIGKYPTNYTLHKSLGDYYLNVQLTDEEGWIMSDSALVIAIKDNYLLAYQHNVSDY
                     KSLNGLGYTAMLEEDFETAIAYLEQSVKLNKNYPVALSNLTDAYFATGKFDNALLMAQ
                     EAFGLYTENTDKAEAARKIAEIYIEKKQNDKALLYYLKADEILPNDYLTLVPVLKLEM
                     ELNNPDYANRTNQIITLAPDNPVVYQDVYKVYSENEKEKEFVAFLESQKVYYRSNVRA
                     QAHIHFYMAVAQYEQDDWVDAKINFEKARNLFRSFFKSNHSVFKVIDSYTNAIRKK"
     gene            5357..5791
                     /locus_tag="PKH58_06905"
     CDS             5357..5791
                     /locus_tag="PKH58_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNX88794.1"
                     /translation="MEITDFIVRLLVATAAGGAVGLERQWNNKTAGLRTNTLVAIGAC
                     IYVLINVFLTEKGADPTRIIAQIVTGVGFLGAGVILHDHGSVHGLTTAATIWCSAALG
                     SLAGLGYYRETAICTVLVLIVNIAFKKADLWFDSEKKKNEGK"
     gene            complement(5798..6625)
                     /locus_tag="PKH58_06910"
     CDS             complement(5798..6625)
                     /locus_tag="PKH58_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018910838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNX88795.1"
                     /translation="MNTQQVVLITGASSGIGKEAARLLAGKGYKVYGAARNVEKMEEL
                     IPLGITVIPLDVTNQSSIENCVDRILTTEGRIDILVNNAGYGTFGPVEAVPLEEGRRQ
                     FEVNLFGMAALIQKILPVMRKQKSGRIINVSSMAAYFSEPNGGWYHATKAAVERYSDA
                     LRMEVKRFNIQVVLIQPGMINTEWQVIAGQYLLKYAANTVYEPSARHQAEMFEMGYKR
                     ASKPVVVAKAIVRASTSRRPCLRYKIGGMAKPILLLRKLMPDRWFDAITAYVMNGKN"
     gene            6912..7193
                     /locus_tag="PKH58_06915"
     CDS             6912..7193
                     /locus_tag="PKH58_06915"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNX88796.1"
                     /translation="MDFKQLNDIKNRFVTRSVGDELVIVPLSGNVAQMNRLFTLNETG
                     KFIWENSDKCADNEAMVQLLTENFEVGQETALSDVETFLDKMNILLSNQ"
     gene            7203..8069
                     /locus_tag="PKH58_06920"
     CDS             7203..8069
                     /locus_tag="PKH58_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88797.1"
                     /translation="MFGQHQVQISVGGFIIRLFSKRQIRLDVGYTHFVCSQKEKPEIL
                     VECLDGVPEGILCGAELVFKAANSEKIFYTIYRTGAGLVFTVYDQHRQDEIQQFALLD
                     DALRQWKIWSKLIGDTLEPLLFPMGAILFHYATLQGNAVMMHSSCIFDGKKGRLFSGF
                     SGAGKSTISGLWVKAGSRMINDDRVIIRKEEEGYMAYNTPMYYADEPKKTRLDAVYLI
                     SHAPKNNMTKRSGALAITGIMAFSIQNNYDVRFVQHHLAFFSEMCLSVPVYDLGFVPD
                     ERVIKFIEENGV"
     gene            8211..10454
                     /locus_tag="PKH58_06925"
     CDS             8211..10454
                     /locus_tag="PKH58_06925"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNX88798.1"
                     /translation="MQKESKYHETLIDLAEVLLEDKNTLSIRVSGFSMYPVLQEGDIC
                     FVEKCSTEELKRGDIIVFRDGKKLIAHRLLHIQKTDNGYLLKTRGDNNRFDDNPFTDE
                     FLTGKLVRYQRNNKTCSFNSLSMRGRRFSAVAFPSFSSFINNTHLRLSRGEKFAVTAV
                     HSLKNNISTILTGSEKLFRINVLISVLQGLIPLAIIVCFKYLVDFLTHNTIQTLQGRL
                     FWFVLLGITGLLFLGGSVFSQLYGYFSEKMSQSVTRHIYASLHRQHVCLGLSAYENPD
                     KQDKMHRAVQEASFRPVKILNAFLTLIKTFSSGIVLVGLFVSIRWYVFLILLIAVIPD
                     ALVRFTFSRKRYHLKEVQTTREREKFYYNRVLTAFPFAKELRLFGFSDFFLKRFNRTQ
                     DSLFDEKIRLSRSELTLTVAAQVFAVLLIFGSLGIVSYLSIQGSMTIGAVVLFFFAFQ
                     RGYSTLNEFFRSVTGLLEDNSFLQDYIDFLDIKPEKEAVAGTKSFSLEHEIRFDKVSY
                     RYENSQRDVLKEVSLRIPTGKTIALVGKNGSGKTTLVKLLCGFYHPESGAILIDGVDT
                     RLIGQQEICEHVAAVFQDFALYQVSAMQNILLGNVGKNPDTDKARKAAAVAGIDETLE
                     KLPNGYQTLLGNLFDGSEELSIGQWQKMAIARAFYRDAPLLLMDEPSSALDAASESQL
                     IGKLKELSHNRTSLIISHRLSTVQWADFIYMLDEGEVAEQGTHSDLMALKGKYYALFT
                     SGREKES"
     assembly_gap    10458..10557
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10558..11244
                     /locus_tag="PKH58_06930"
     CDS             <10558..11244
                     /locus_tag="PKH58_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX88799.1"
                     /translation="IPDAVIKKGDLLIITVNSVTPELSRPFNLSLLPTTGMGEYSQTN
                     AMGTTGTSGSLQNYLVDNQGFITFPLVGKIAAEGMSKNALSERIKSLIYPRYITEEPI
                     INIRYADFSISVLGEVSRPGSFRINNESCSVLEALALAGDLSINGKRDNVLLIRQTRD
                     ARESVRLDLRDKNLINSPYYFLQQDDVLYVQPSDTKARSSKIGTAETLSLSVVSTLIS
                     LTTLIISIAK"
     gene            11314..13707
                     /locus_tag="PKH58_06935"
     CDS             11314..13707
                     /locus_tag="PKH58_06935"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNX88800.1"
                     /translation="MDNNIYDLLEEASLRQENSQNPIDWRELTEKVLEHWKWIVLCVV
                     VALITGYLYNRKQMDLYSVKTSMLVQDQSKSSGMNEMSLLRQLDATGLRSGTNYSFLN
                     NENAVLRSSLLMKNVVNQLELYTDYSTTSFLKKKDLYTSSPLLLTIDSVSLNKLEDLL
                     ILKVTPNGKGFHVEGEYKGTDFEQQASSLPAEIKTPAGVVFLNARAGAERPKEIITIT
                     VRNPAATTKYYTNTVLKTDLNNKDKVDVIDLSVSVSNVQKGKDILNTLINDYNQDAID
                     QVNKSANNTARFIDSRLQIIYGELSDEEKRVENYKQSNNMTNVESDVALYLQQGDRFN
                     QELMEVETQLKLIRFVEDFIKDPANNSSPIPNLGVKDNGLTSVIQEYNQAIITRERIA
                     AGSSAENPVLRDLNKQIELGRNAIRTSIANNRRGLEISRKDYSSRNSEAQARINELPR
                     QEREFLDIKRQQQVKESLYVFLLQKREEASLTMAVTVPKGRVLDNPDYAGKIAPNSKM
                     IMLIFLMLGLMLPLAYVFLKDMLQTKLITRKQLEKMTDVPVLSELSHNTTGMRVIDHH
                     AFNDSNVELFRLLRTKLNFVLQQPAQKVVTVTSTEPGEGKSYVAINLAINLSLSQKKV
                     ILLGADIRKPQLKNYLKMKQVDLGLTSFFSGQVKDYHTLITNLDDYPSLHVIIGGIVP
                     PNPNELLMSDRLDFLIAELRKEYDYIILDTAPVGVVSDTFVINRISDISLYVIRMNYS
                     DKRNIDYLNHVVDDKTLTRPYIVLNDLDIEAKYSYRKGYGYGYGYYYGHNKTKESNE"
BASE COUNT         4110 a         2855 c         2972 g         3750 t
ORIGIN      
        1 aatttttagc tttttatcga cagcggcggc cttttttgtt tactttttgg ggctgtagcc
       61 aattggagcg aagcggaaat cgccgaaaca aataaaatac aaaactactg aggcaaaaag
      121 taaaagccag tccggctaga ggacataaaa gaactttttt aacctgacaa tagttttatt
      181 taaccaatta attaaaaata aacagatatg aaacgtacga ttttgttttt tacattcacg
      241 gcattagttt ttagcctgag cgctcagaat tcggtagtta tcaaactcaa cgaacacacc
      301 aaataccgtg gttacaacac catggaagct tttcagaaac gtcgttcggt caatatctat
      361 gaaacccgtg acctcagttt gcaggatttg tcggatctgc tttgggctgc caacggtatc
      421 aaccggcctg aaaacgggaa gaaaacagcg ccttcagcga tgaactcgca ggatgtggat
      481 atttacgtca gccgtgccaa tggtacctac ctgtacaatg caattacaaa tgacctggta
      541 ctggttacca ccgaagatct tcgtccgctt atggatggca atcgcccgtt gggtacacct
      601 gttatcctgc tgctcgtcag cgatatgtcg cgctacaaaa attattcgcc cggcaatccc
      661 gatcaaaaca ggcatctctt tgaagtggga gccattgatg ccggaattgt ttcgcagaat
      721 atctccctgt tttgcgctgc caatgatctg ggaacacgcc cgcgggcaag catgaatcag
      781 gtcgcgctcc gcaaagccct gaatctgaaa gaatcgcagg tcatgtggct gaatcatccc
      841 gtaggctatc cgaaataacc tcatataaac aatattatga acaagagaaa atgtacacaa
      901 atagtccgcg ggcagcaagc cgttgacgga gcaggagttc atctgcagcg tgtgttggga
      961 ctgagaacag tgaaggattt tgatccgttc ctcatgctcg acggattcga cagcagcaat
     1021 cctgccgatt acatcaaagg atttccctgg cacccgcacc gtggtatcga aacagttact
     1081 tacctcatca gcggtgaaat agaacacggt gacagtcttg ggaacagggg aactatcaga
     1141 gacctggaat gtcagtggat gacagcgggc tccggtatca ttcatcagga aatgcccgtt
     1201 gcatccgaac gcatgctggg ttgtcagttg tgggtaaacc taccggcaaa agataaaatg
     1261 accgatccgg cgtaccgcga catcacacag aaggatgtgg ctactgtcga agatgaaaac
     1321 gctgccattc gtgttttgtc cggcagttat aaaaatcaca ctgcagccgt aagcggaaac
     1381 tatgtgaagg taacgtatct ggatgtacag ttaaaacccg gcacaaagtg gacttacaac
     1441 gaaactccca acgatgaaac cctgtttatt tacctgattg atggtacgct ggctcccgcc
     1501 ggagaaggag ctgcattcga agaaaaggct tgtgccattt tatttactgc ttcgaatgaa
     1561 ctatcgtcag actttgaaga acttcaggta aaagcaggca gtaatggtgc ccggtttttg
     1621 cttctggcag cccgcccgct gaaggaaccg gtagcatggg gcggacccat cgttatgaat
     1681 acacgcgaag aactcaatca ggcatttgat gaactggaaa ccaataattt catcaaacat
     1741 aagatgtaag taaacactga aaactgaacc ggataacaaa aaagacaggc tgacagggat
     1801 actttttcaa actgtcagac ctgaatttcc gataaaataa ctaattttgc aaaactattc
     1861 cggaatccgg ttgtgacaat aagtctgcca atcacggaaa tgaatgatca ccggacaatt
     1921 ttatgtttgt acggtcgtta taaatacaat tttgagtttt cgggaacaaa aaaaaagaaa
     1981 aactcactgt attacgtaaa cacaaattta tcttacatat gtcactgaaa aaaacagtta
     2041 ttactttcct tctgctcgca ggcattcagc ttgccggtgc acaaatcaga cccaacgaaa
     2101 cactggtata taacggttct taccaactga gcggtatgat gaccagcctg gctcaaatca
     2161 cgatgaaaac ggagccgatc aaaacgagtt caaaatctta cctgcgcctg tgcgttcagg
     2221 tggctaccta caccaagtgg gattcctttt tcaaaatccg tgacctgtac gagtcttatg
     2281 tacatcccta ttcgttgaaa ccaacgcttt ttaagcgcaa tgtttatgaa ggcggataca
     2341 ccaagcagga aaagtacaca ttcaatgcag caggaagtca ggtttcagct tattcaaaac
     2401 gaagagacaa accggcagaa accgaaaatg taaatatcag tgccaacact ttcgacgcgg
     2461 taacgcttat gtacaaactt cgtacggtcg atttgtcgaa aatggcaaaa ggacagtcac
     2521 ttcgtttttc cttgatattc gataaaaagg aacattttat ctggatcaag atgatgggaa
     2581 aagaaaccgt ttcggcaggt aatctgggta aaaaggaatg tttcaaactt tccatctcag
     2641 ctaaaaccga taagctgaga ggagtggata aaaatctggt gtggctctct acggatgcca
     2701 aacgaatacc gtgtatgatg aaattcagca ttccggtagg tatcggacaa gttacacttt
     2761 ccaaagctac aggaatttaa tttatttatt atgagaaaac tatttaatat tctggccgtt
     2821 gtatttgcgg cattaggact tgtgtttgcc atcttgccca tgggtagtat tgcattctta
     2881 cctataatac tggcaataat actggcactg cttgccatgt ggaaatcgaa tgatactcaa
     2941 aagaaatttc ccaaatgggt tctgattatt tcaatggcta ttttgctgtt tgttaccgtt
     3001 aaagtggttt ttgttaagga taaggtggtg gttgacgaac agttcatgca ggaaaatgtg
     3061 gattcaaaat ctgaagctca gaaggaactc gaaggattgt aaataacatc tgtaaactct
     3121 tttccggata taaccaacaa atttactttg attatatccg gattttttat gtccgggtgg
     3181 ttcatttcat agaattataa tgagctatcc taaaattgaa tggtctctta atttttgaaa
     3241 gctatacgac atgtttaaaa taaatgtata tctttgttat attcaattca gcaatatact
     3301 cagccggtcg tacatgatga tctgatgata atctgatgat tataagcctg aataatgaga
     3361 aaaagcctgc gaaagctgtt tattatggca acgaaggaca taccatccat accaggtcaa
     3421 atgttccgat aaaacggttg tttattgaca atgataaaaa tgctgaagca ccttttttcc
     3481 ggctggtcta taaataaatc gatccggaaa ccatcctcac aagatttaag gtttcaatgg
     3541 atggaaaaaa tttcagcacg tatgtggaag ggaaaagcaa aaaaggtaca ttccggcatc
     3601 ctgaaataaa taatatgagg caaacacctt tattcaccaa aataaaaacg gataattaac
     3661 cgttatgcaa tgagaaagga aaatcaggca tatgttttgt atcaatggct aatagcatac
     3721 tggtaaatta aaattattcc accggttttc tggcaatcaa accatgatta tttttagaat
     3781 gagatttttt gtatcaatca tactactctt ttgcgctgca ctggcacttg cacagtcggg
     3841 cccaatcagt cattccggcg aactgatagc ccaaaagaga tataaatcgg cgtatcaggt
     3901 cctcgatagc gccgatacag ttaatcagaa cccggaaata gtcattgcca aaacagacct
     3961 gctgctcaac tataatatcg gtgtcaacaa ccatcgggta tttgcactga atgatagtgc
     4021 acctagccag tctgtaattc aaaacaggcc aatatatgta aactttcctg cagacagtat
     4081 cctgaatgaa ttaattggca aatatccgac taattacacg cttcataaat cgctcggcga
     4141 ttactacctc aatgtacagc taaccgatga agagggctgg atcatgtccg attcggcttt
     4201 ggtgatagcc atcaaagaca attatctgct ggcctatcag cacaatgtat ctgattacaa
     4261 atcactaaac ggtttaggtt atactgccat gctcgaagaa gattttgaaa ctgccattgc
     4321 gtacctggaa cagtctgtga aacttaataa aaattatcca gttgcgcttt ccaatctgac
     4381 tgatgcctat tttgctacag gtaagtttga caacgcgttg ctgatggctc aggaagcatt
     4441 cggattatat accgaaaaca ccgataaagc cgaggctgcc cgtaaaattg cagagattta
     4501 tattgagaag aaacaaaacg acaaggcttt gttgtattat ctcaaagccg atgaaatact
     4561 tcctaacgac tacctgacac tagttccagt actcaaactg gaaatggaac tgaataatcc
     4621 cgattatgcc aatcgtacca atcagataat tacacttgcc cccgacaatc cggttgttta
     4681 tcaggatgtg tataaagtgt acagcgagaa tgaaaaggaa aaagaatttg tagcttttct
     4741 ggaaagtcag aaagtatatt accgctccaa tgttcgggca caggcacaca tacatttcta
     4801 catggctgtg gcgcagtatg agcaggacga ctgggtggat gccaaaatca attttgaaaa
     4861 agccagaaat ctgtttcgga gctttttcaa atccaatcac agtgtattta aggtgattga
     4921 ttcatacacc aatgccatcc ggaaaaaata actgttcagg ggagattggt tattcgttat
     4981 tgagttatta agttataatc ggtgaattga tgtactaatg tatcaatttg aagatttgaa
     5041 aatgtgccaa tttgataatg tttaattgtt taatcgttga ttcgttgaat tgttgaattg
     5101 tgtaatgttg aatgtgtagt gtgtagcatg gaacgctgag cctgtcgaag catgtactgt
     5161 tcactgttca ctgattactg attactgatt gctgattatt gattgctgat tactatatct
     5221 tacttgataa actttataaa cttgatgaac ttttcactat aaactattga ttattgactg
     5281 ttttttactt gatgaacttg atgaacttta tgaacttttc actataaact gtctcgtaac
     5341 tttaaaaaat acttccatgg aaattaccga ttttatcgta cgtttactgg tagccacagc
     5401 agccggagga gctgttggtc tggagcgtca gtggaacaat aaaacggccg gattgcgaac
     5461 taataccctg gtggcaatcg gagcatgtat ctatgtgctg atcaatgtat ttcttaccga
     5521 aaagggtgcg gatccaacgc gtataatcgc tcagatcgta accggtgtcg gctttctggg
     5581 tgcaggggta attctgcatg atcacggtag tgtacacgga cttacaacag cagccaccat
     5641 ctggtgcagc gctgcattag gctcccttgc cggacttgga tattaccggg aaacagctat
     5701 ctgcacagtg ctggtgctga ttgtaaatat tgctttcaaa aaagcagacc tctggtttga
     5761 ttctgagaag aaaaaaaacg aaggaaaata aataatctta gtttttaccg ttcataacgt
     5821 atgcggttat cgcgtcaaac cacctgtcgg gcattaattt tctcaataaa aggattggct
     5881 tagccattcc accaatttta taacggagac agggacgacg actggtactg gcacgaacaa
     5941 ttgctttggc tacaaccaca ggtttcgaag cccgcttata tcccatttcg aacatttctg
     6001 cctgatgacg ggctgatggt tcgtaaaccg tattcgccgc atatttcagc agatactgac
     6061 ccgcaatgac ctgccactct gtattgatca taccgggttg aatgagaact acctgtatgt
     6121 tgaaacgttt cacttccatc cggagtgcat cgctgtaacg ttccaccgca gcttttgttg
     6181 catgatacca gcccccgttc ggttcactga aataggcagc catcgatgaa acgttgatta
     6241 tacggccact tttctgctta cgcatcaccg gaagtatttt ttgaatcagt gccgccattc
     6301 caaagagatt tacctcaaac tgacgcctgc cttcctccag aggaaccgct tccaccggac
     6361 caaaagtgcc atatccggca ttattgacaa gaatatcgat acgaccttct gtcgtcagaa
     6421 ttcggtccac acaattttca atggaggatt gattggttac atccaaagga atgacagtga
     6481 tacccagcgg gataagttcc tccatttttt ctacgttacg ggcagctcca tatactttat
     6541 aaccctttcc ggccaataat ctggcagctt ctttacctat tcccgatgat gcaccggtga
     6601 ttaaaactac ttgttgagta ttcatatttt tctgatctat tattttttaa ttacaggtga
     6661 gccaacgaaa aacctgtaac gattgttcat tgcagaccaa ttatgcaacg aaattacgga
     6721 tttttttgaa gaaaaccttt atattttaat attctcaatt accttttaca aatcaccaat
     6781 cttcaaatct tcaaattggc atattagtat atcaacaact caataaccat taacttattc
     6841 tccttcatac tagtgtcttt tacaaaaaaa acatatcttt gtaagtatta aaaaataaaa
     6901 tttctggtat tatggatttc aagcaactta atgatattaa aaatcgtttt gtaacacgct
     6961 ctgtgggcga cgaactggtt attgtgccgc taagcggaaa tgtggcacag atgaaccggc
     7021 tttttacact gaacgaaacc ggcaaattca tctgggaaaa ttccgataaa tgcgctgaca
     7081 acgaagcgat ggttcaactg ctgaccgaaa atttcgaagt cggacaggaa acagccctca
     7141 gtgatgtgga aacctttctg gataagatga acatactctt gtctaatcag taattaatgc
     7201 ttatgttcgg tcaacatcag gttcagattt cggtaggagg atttatcatc cggctttttt
     7261 ccaaacgtca gattcgtctc gatgtaggtt atactcattt tgtgtgtagc caaaaagaga
     7321 aaccggaaat tctggttgag tgtctggatg gagtccccga aggtattctg tgcggtgcgg
     7381 aacttgtttt taaagctgca aattcagaaa agatattcta tacaatttat cgcacgggag
     7441 cagggttagt tttcacggtt tatgaccagc acaggcagga tgaaattcag caatttgcac
     7501 tgttggatga tgctctcaga cagtggaaaa tctggtcgaa actgatagga gacacattag
     7561 aaccactgtt attcccgatg ggagccattc tttttcatta tgccacattg caggggaatg
     7621 cggttatgat gcattcatcg tgcatattcg atggaaaaaa aggacgctta ttcagcggtt
     7681 tttcgggagc cgggaaaagt accatctcag gtttgtgggt caaagcagga agccggatga
     7741 ttaacgacga ccgcgtcatc atccgaaaag aggaggaagg ttacatggct tacaatacac
     7801 ccatgtacta tgccgatgaa ccgaaaaaga cccggttgga tgcagtatat ctcatcagtc
     7861 atgctccgaa aaataatatg acaaaacgca gtggtgctct ggcgataacc gggattatgg
     7921 ctttcagcat acagaataat tacgatgtac gctttgtaca gcatcatctt gcttttttca
     7981 gcgagatgtg cctctcagtt ccggtttacg atctgggctt tgtgcccgat gaaagagtga
     8041 taaagtttat tgaagaaaat ggagtgtaga ttttttgtgg ttagtaatta agcctccctg
     8101 tcccatcatg gggttcaaga ttaggtagtg attagttatt atcaaaacaa cgattaaacg
     8161 attaaacatt acacacttca ccaaagacct gttcttatct caaaatattc atgcagaaag
     8221 aatcgaaata ccatgaaaca ctgattgatc tggcagaggt gctgctggaa gataaaaaca
     8281 cgctcagcat tcgtgtgagt ggtttcagca tgtatcctgt tttgcaggag ggtgatatat
     8341 gttttgtgga gaaatgcagc acagaggaac tgaagagagg tgatattatt gttttcagag
     8401 atggaaaaaa gctgatagct cacaggttac tgcatattca aaaaacggat aatggttatt
     8461 tgttgaaaac cagaggtgac aataaccgct tcgacgataa cccttttact gatgaatttt
     8521 taacaggtaa actggttcgt tatcagcgga ataataaaac ctgttctttt aacagcctca
     8581 gtatgagggg tcgcagattt tccgctgtag ctttcccatc attttcttcc ttcatcaata
     8641 atacacacct gcggttatca agaggcgaaa aatttgctgt aacggctgtt cattcactga
     8701 aaaacaatat ttctacaata cttaccgggt ccgaaaagct ttttcgcatc aatgtgctga
     8761 tcagtgtcct gcagggattg ataccactgg caatcattgt ttgttttaag tatctggtgg
     8821 attttctcac acataacacc attcagacgt tgcagggaag gctgttctgg ttcgtactgc
     8881 tgggcattac cggtttactt tttctgggag gttcggtttt tagtcagctt tacggttatt
     8941 tttccgaaaa aatgtcacaa tcggttacga ggcacatata tgccagtctt caccgtcagc
     9001 acgtatgctt agggttgtct gcctacgaaa atccggacaa gcaggacaaa atgcaccggg
     9061 ctgtacagga agcatcattc agaccagtaa agattctgaa cgcatttctc acactgatta
     9121 aaactttcag ctcgggaatt gtcctggtcg gcttgtttgt gagcatccgg tggtatgtat
     9181 ttcttattct gctgattgcg gtcattcccg atgctctggt acgatttacg ttttcacgaa
     9241 agcgctatca tctgaaagaa gtccaaacta cccgtgagcg ggagaaattc tattacaacc
     9301 gtgtactgac agcttttcct tttgccaagg aattacgtct ttttggtttc tccgatttct
     9361 ttctaaaacg gttcaatcgc actcaggaca gccttttcga tgagaaaatc aggttaagcc
     9421 gctcagaact tacccttacc gtggctgcac aggtttttgc ggtactgctt attttcggat
     9481 cgctgggtat tgtgtcgtac ctgagcattc agggctcgat gacaataggt gcggtggtgc
     9541 tttttttctt tgcttttcag cgcggatatt ccaccctcaa cgaatttttc cgttcggtta
     9601 ccggactact cgaggataac agcttcctgc aggactatat tgattttctt gatattaaac
     9661 cagaaaaaga ggccgtagcc ggcacaaagt cgtttagcct ggagcatgaa atacgtttcg
     9721 ataaggtaag ttatcgctac gaaaacagtc agcgcgatgt tctgaaagag gtatcgttac
     9781 gcatcccgac cggaaagacg attgcactgg tgggaaaaaa cggttccggt aaaacgacgc
     9841 tggtcaagtt actgtgcgga ttttatcatc ccgaaagcgg cgcaatactt attgacggag
     9901 tggatacacg cctcatcgga caacaggaaa tttgtgagca tgtagctgct gttttccagg
     9961 atttcgcgtt atatcaggtt tcagctatgc agaatatact tcttgggaat gtcgggaaaa
    10021 atccggatac cgataaagcc cgaaaggctg cagcagttgc cggaatagat gaaactctcg
    10081 aaaaactacc taacggatat cagaccctgc tgggaaatct gtttgatgga agcgaggaac
    10141 tgagcatcgg acaatggcag aaaatggcaa tagccagagc tttttaccgt gatgcaccat
    10201 tgctgcttat ggatgaacca tcgagtgcgc tcgatgcagc ctccgaaagt caactgattg
    10261 gcaaactgaa agagttgtca cataaccgca cttcactcat catcagtcac aggctcagca
    10321 ccgtgcaatg ggctgacttt atttatatgc tcgatgaagg tgaagtggcg gaacagggta
    10381 cgcattccga tctgatggct ctgaaaggga aatattacgc tttatttacc agcggaagag
    10441 aaaaagagag ttgagaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnata
    10561 cccgatgccg tgattaaaaa aggcgatttg cttataatta cagtcaattc tgttacaccc
    10621 gaactgtcca ggccttttaa tctttccctg ttacctacaa ccggaatggg ggaatacagt
    10681 cagaccaatg ccatgggaac tacaggaact tcaggtagcc ttcagaatta tctggtggac
    10741 aatcagggct tcatcacctt tccgctggta ggtaaaattg ctgctgaggg aatgagtaaa
    10801 aatgcattat cggaaagaat aaaaagtctg atatatcccc gatatattac cgaagaaccg
    10861 attatcaaca tccgctatgc cgatttcagc atttcggtat tgggggaagt cagtcgtccc
    10921 ggatcgttcc gcatcaacaa cgaaagttgc tcggtgctcg aagcactggc actggcaggc
    10981 gacctgagta taaacgggaa gagagacaat gtgctgctaa tccgtcagac ccgcgacgcc
    11041 cgcgaatctg tccggctgga tctgcgcgat aaaaatctta ttaactcccc ttactacttt
    11101 ctgcagcagg acgatgtgct ttatgtgcag ccatcggata ccaaagcacg gagttcgaag
    11161 attggtactg ccgaaacact atcactgtca gtcgtaagta cactcatatc gctcacaacg
    11221 cttatcattt ccattgccaa atagaaaaca aatcaactct ttcacagtat aactcctgtg
    11281 tttatctctt actgaaatat aactgataat agtatggaca ataatatata tgaccttctg
    11341 gaagaagctt cgttaagaca ggaaaacagt cagaatccca tcgactggcg tgaactgacc
    11401 gaaaaagtat tagaacactg gaaatggatt gttctttgtg tggtggtggc gctcataaca
    11461 ggttacctct ataacagaaa gcaaatggat ttgtattcgg taaaaacctc gatgctggta
    11521 caggatcagt ccaagagcag cgggatgaat gagatgtcgc tgctgcgcca gttggatgca
    11581 acaggtctcc gcagcggtac caattattct tttctgaaca atgagaatgc tgtactgcgt
    11641 tcgtcgctgc tgatgaagaa tgtggtcaat cagctggaac tttacaccga ttattccacc
    11701 acgagttttc tgaaaaagaa ggatttgtac acttcatcac cgttattact caccattgac
    11761 agtgtgagcc ttaataaact ggaggactta ctaatactga aagttacccc gaacggaaaa
    11821 ggttttcatg tggaaggtga atacaaaggt accgatttcg agcaacaagc atcatcctta
    11881 ccggccgaaa taaaaacacc tgcaggagtc gtgtttctca atgcccgtgc cggagccgag
    11941 cgaccaaagg aaattataac aatcaccgtg cgaaatcctg cagctactac aaaatactat
    12001 accaacactg tactgaaaac agacctgaac aacaaggata aggtggatgt gattgatttg
    12061 tcggtgagtg tttccaatgt tcaaaaaggt aaggatatcc tgaacacact tatcaacgat
    12121 tacaatcagg acgcaattga ccaggtaaat aagtcggcaa acaataccgc aaggtttatc
    12181 gacagccgat tacagattat ctacggcgaa ctgagtgatg aagagaaaag agtggagaac
    12241 tacaagcaat ccaacaatat gaccaacgta gaatcggatg tcgcgcttta tctgcaacag
    12301 ggcgaccggt ttaaccagga actgatggag gtggaaactc agttgaagct catccgtttt
    12361 gtagaggatt ttatcaaaga tcctgccaat aattcttcac ctatccctaa tctgggagta
    12421 aaggataatg gattgacttc ggttattcag gaatacaatc aggctatcat cacccgtgaa
    12481 cgcatcgccg ccggctcatc agccgaaaat ccggtgctgc gtgatctgaa taaacagata
    12541 gaattgggcc ggaacgctat ccggaccagt attgccaaca accgcagggg tctggaaatt
    12601 tcgcggaaag attacagttc gcgcaactcg gaagcacagg ctcgtatcaa tgagttgccg
    12661 cgtcaggaaa gagaatttct ggatatcaag cgtcagcagc aggtaaagga atcgctttat
    12721 gtatttctgc tgcagaaacg cgaagaagct tcgcttacca tggcggtaac tgtacctaaa
    12781 ggacgcgtac tcgacaatcc cgattatgcc ggaaaaatag cgccgaacag taaaatgatc
    12841 atgctgatat tcttaatgct cggattgatg ttgccattgg catatgtttt tctgaaagat
    12901 atgctgcaga ccaagctgat cacccgtaag cagcttgaga aaatgactga tgtacctgtc
    12961 ctgtccgagt tgtcgcacaa taccacgggt atgcgtgtaa ttgatcacca tgctttcaac
    13021 gattcgaacg ttgagctgtt ccgcctgctc cgaaccaaac tgaactttgt actgcaacag
    13081 cccgcacaga aagtggtgac tgtaacctcc accgaaccgg gtgaaggaaa atcatacgtt
    13141 gccatcaacc tggccatcaa cctctcgctt tcgcaaaaaa aggttattct gctgggcgcg
    13201 gacatccgta aacctcagct taaaaattat ctgaaaatga agcaggtaga tttggggctg
    13261 accagctttt tctccggaca ggttaaggat tatcacaccc ttattaccaa cctggatgat
    13321 tatcccagtt tgcatgtgat tatcggaggt attgtaccac ccaatcccaa cgaactgctg
    13381 atgagcgaca gactggattt cctgattgcc gaactccgca aggaatatga ctatattata
    13441 cttgatacag cacctgtcgg ggttgtttcg gatacatttg tcattaaccg tatttcggac
    13501 atctcgctgt acgtaatccg tatgaactac tccgacaaac gaaacatcga ttacctgaac
    13561 catgtagtgg atgacaaaac gttgacacgt ccatatattg tgctgaacga cctggatatt
    13621 gaggcgaaat actcctaccg caaaggttac ggatacggat acgggtatta ttacggtcac
    13681 aacaaaacca aagaatctaa cgaataacct gataaaataa atatatcaca ataaagcgtc
    13741 ggaaaatctc caacgctttt ttttatgaaa atgaaactgg tgagaaa
//
LOCUS       DAOPYB010000070        13574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_4977_length_13574_cov_9.363267, whole genome shotgun sequence.
ACCESSION   DAOPYB010000070 DAOPYB010000000
VERSION     DAOPYB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13574
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4977_length_13574_cov_9.363267"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PKH58_06940"
     CDS             complement(<1..967)
                     /locus_tag="PKH58_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HNX88801.1"
                     /translation="MKKYTRNIRFLVAATVTVAALYACANRGQGPQGGPKDETPPSVV
                     KSFPNNKSLNVTKGKVEIIFDENVNVLKASDNVIISPPQRIAPEVKSYAKTVTVQLND
                     TLIPNTTYSINFGDAIVDNNESNPLKNYFFAFSTGNEIDTMQIAGTVIDAQNLNPVPG
                     VTVGIYDDLSDTVFMKKPFLRISRTDNMGRFTVPNVRNGKYRVYALTDGNRDNFYQPG
                     EALAYSQSVFTTTFENYMRSDTIWKDSVTVDTIMSVPAIRYLPDNVVLHYFKDETTRQ
                     YLVKSERTVPHRISMYFNTAAEHLPEIKALNVADWDKKILLQKNEK"
     gene            complement(964..2238)
                     /locus_tag="PKH58_06945"
     CDS             complement(964..2238)
                     /locus_tag="PKH58_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNX88802.1"
                     /translation="MKESILHYVWQYRLFEATQLTTTRGDSVEVIDPGKLNTDAGPDF
                     FNAKLKIDDTLWAGNIEIHNQSADWKKHNHQNDKAYDNVILHVVQKADVDVFRSNGEL
                     IPQMELAVPVDIQTRYDDLITSRKWIACEDKIHQVPSILISSWKTTLLTERLERKVAA
                     IETMLSQSQNHWEDAFYISLARSYGFSTNSHAFEAMARSLPLAVLAKHKDNIFQLEAL
                     LMGQAGFLEKPVEDKYPLELQKEYRFLQTKYGLNPIEVSQWKLLRLRPENFPTIRLAQ
                     FASLIHRSSKLFSLILETPDCQDLRKLFDTEVSEYWQKHFLFGRESRVSRKIMGNKSI
                     DILLINTVIPFIFAWGKKKNDENRTEQAIRLLEEIKPESNSIVHRWEKLGINCKSAFD
                     SQALLELKKNYCEEKKCLLCRIGHKVLIINKQ"
     gene            complement(2304..2912)
                     /locus_tag="PKH58_06950"
     CDS             complement(2304..2912)
                     /locus_tag="PKH58_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX88803.1"
                     /translation="MLQVPEIIKSLFKNVVWRINPAGKAIYLTFDDGPHPQVTPQVLD
                     ILDRYGVKATFFCVAENVSRYPEVFDEVKRRGHAVGNHTYNHLKGFDTPDDVYLKNIE
                     KASEYIDSKLFRPPHGRIKLSQIKALKKNFRIIMWDFITYDYDKNIEPERIVNEVKRR
                     SRNGSIVVFHDSLKAEKNMLSALPEALQYWLDEGYNVERISG"
     gene            complement(2973..6200)
                     /locus_tag="PKH58_06955"
     CDS             complement(2973..6200)
                     /locus_tag="PKH58_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNX88804.1"
                     /translation="MKKFRFLNNIFGWVAFAVAAVTYLLTIEPSASFWDCGEFISSAY
                     KLDVGHPPGAPFFMLTAKFFTLFASDPTKVAMMVNSFSAIASAFTILFLFWTITHLAQ
                     QVIGKREEEYSAANIIGILGAGMVGALAYTFSDTFWFSAVEGEVYAYSSLFTAVVFWL
                     ILKWERVADHKGSDKWLILIAYLMGLSIGVHLLNLLAIPAIVLVYYFKKYKVNVKGSM
                     LALLTSVVILGLVLYGLIPGFVVVASKFELFFVNTLGMSFNSGVYVYILILVAAIVWS
                     LYESFTEKNYTRMAISYMLAVTLAGIPFIGENIFLGIVIIAGMVFFFFRKKKNISARW
                     LNTSMAMIAVMLIGYSSYSIIMIRSAAQPTMDQNSPDNIFALKSYLNREQYGDRPLIY
                     GAVFSAPLKLDVQGNTCVPIADKTPIYCPKPKKDASEKDEYIVSGYKSDYKMDSRFMM
                     LFPRMYSSDKSHVSYYTTNYVRSGKKISYDFCGQEKSDVMPTFGENLNFFLDYQVNFM
                     YWRYFMWNFSGRQNDIQSYGEIEHGNWITGIPFVDNMLVGNQSNLPTELKENKGHNVY
                     FMLPLLLGILGLFFLLNSGKKGNHTFWITGLLFVLTGLAIVVYLNQTPYQPRERDYAY
                     AGSFYAFSIWIGLGVLGVMQLLDRFLPKRVSAMLAAVACLGVPVLMAAQNWDDHDRSN
                     RYTARDFGQNYLNSCKPNAIIFTNGDNDTFPLWYNQEVEGVRTDVRVCNLSYLQTDWY
                     IDQMKRASYKSTPLPISWEPKDYISGKNEVMQVEDVLQGQSLDMKTAFDFIKSEDPNT
                     KIEGDGFFPSKQLFLPVNADEIVKNGTVAVKKKAEIVPKMVFDLPSRLTKSETMMLEM
                     IKENNWKRPIYVAVTVAEEYYPSSVTKYFERTGLAYHVVPVAQKVEGVTNVNTDEMYE
                     NLMTKFKYGGIDNPKVYIDDNIMRMCRTHRAMFAYLIEALVNEGDTVRARKAYKYCEK
                     VVPAATVPHDYSSNMLAHYCFKLGLINEGNKILDDVAKTSVEYLTWYASLTPSQQNSS
                     GSSIGRNLAILNNVLQISQETPGKKIFNKYLPVFETFANKYQSAQ"
     gene            complement(6393..7052)
                     /locus_tag="PKH58_06960"
     CDS             complement(6393..7052)
                     /locus_tag="PKH58_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88805.1"
                     /translation="MKKLIFILCIGFLIAACGQKQSDKTKTDEKATTESVAQVKKGKK
                     YPIEKGIVYQKTEVMGIESNPVIYFDKWGEWETTETTTSMEMLGQKISTTSLKIVKGE
                     DNWEIDMDKKTGFHYNRAVMINQLGVDMENLTTEMMGKMKLEKVGEEKYLGYPCVKYR
                     MKNDELKMDVEYLACGNLMMKMAGKAMEIPTKIEVTKIEAVSPPADKFEIPAGVKITE
                     Q"
     gene            complement(7456..8505)
                     /locus_tag="PKH58_06965"
     CDS             complement(7456..8505)
                     /locus_tag="PKH58_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88806.1"
                     /translation="MKTTSVLFVLILMALTGTITAQKTAFEYLKNVPASPDSVCKCSQ
                     EIRQNYETSLSSLYDIVEEDISKRSRANEDYMSGNEDRIKASMMKEITAKSGISQQEL
                     EAMSKKKKMTQEDKQALANKVLQQHNLSMDEVKNVKNMDENSRKAWAQAYGAEQMAMA
                     QANKNNPYIKATNDKAMNQVALLQEQEKVRNQLSSFETEIANRYATLQNTSGKEKLET
                     DLQKLNDELRSITSIYECNECPAPPSEDIKHWNAVIRKIHDTKITYCKQNTLKMIAYL
                     DWYKENLIKNIPVYDRAEEIQYQLTSSATNTQLNMPAKGIYSLQALAGYLSSKQKLFE
                     FKNFDMQNFASEFMK"
     gene            complement(8590..9873)
                     /locus_tag="PKH58_06970"
     CDS             complement(8590..9873)
                     /locus_tag="PKH58_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX88807.1"
                     /translation="MKRILVLVCICMFFLLPTSAQGWIERAGQRVKNRVVEKVENRID
                     QKVDNATDKVLDSVEKPQKKQTKNNDNGEEEVAEDVGNTDGKVKAKSESPQKLESVTA
                     YDFVPGDKILFFEDFSQDAIGDFPALWTTNGSGEVKTVNKAPGKWLHMNGEDAVYCYT
                     KEIAFPDNFIVEFDLIPDDDFGYGICLTLYQENPENPKEVNSDLYPGLRGLHISPMKD
                     GWETKGYDTDKDWLTGQGSKAPVVTGEPNHVIVWIQKRRVRIYHQGQKVIDVPTNIYA
                     ETKFNKLLFSGWDRHSAPFVTNIKITTASPDTRSKLITEGKLVTYGITFDVNKADIKP
                     ESYGTLKSIADVLTENPGVKVKITGHTDSDGDDARNLDLSKRRAESVKNELNAKFGID
                     ASRLTTEGAGETKPVAPNDTPENKAKNRRVEFLKQ"
     gene            complement(9952..10533)
                     /locus_tag="PKH58_06975"
     CDS             complement(9952..10533)
                     /locus_tag="PKH58_06975"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNX88808.1"
                     /translation="MNLINRVKSILMAPKTEWPIIETEQTDVKTLTINYLLPLALITA
                     VASFIGYGLIGQNVFGVHVGSIGYGLRYALISLVSTIGGAFLTAYIIDLLASSFGSEK
                     NFLQSFKLVVYSYTPMLVAGILLIIPTLGILASLAGLYGLYLLFLGLQPLKKTPEDKK
                     VTYFVISLIVVILGSIVLSAILASIFITSTVFY"
     gene            complement(10834..11379)
                     /locus_tag="PKH58_06980"
     CDS             complement(10834..11379)
                     /locus_tag="PKH58_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNX88809.1"
                     /translation="MKEEILISGFGGQGVLSMGKILVYSGMMQGQEVSWLPSYGPEQR
                     GGTANVTVILSDERISSPILNNYDTVIVLNQPSLEKFESKVKPGGTLIFDSNGFRKFP
                     TRTDINVYRIDATEYAFANNASKTLNMIILGGLLKIRPIVKVENVLKGLKKSLPERHH
                     HLLPQNEEAIKVGMDLIQKYN"
     gene            complement(11475..>11846)
                     /locus_tag="PKH58_06985"
     CDS             complement(11475..>11846)
                     /locus_tag="PKH58_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX88810.1"
                     /translation="NGIYGMTGGQMAPTTLVGMKTATTPYGRNVELNGYPLNITPMLA
                     QLDGTCYVTRQSVHNVANVRKTKKALRKAFENSMLKKGTSIVEVVSTCNSGWKLSPEA
                     SNDWMVENMFPKYPLGDLKDI"
     assembly_gap    11847..11946
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11947..12110)
                     /locus_tag="PKH58_06990"
     CDS             complement(<11947..12110)
                     /locus_tag="PKH58_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HNX88811.1"
                     /translation="MNTEQIINPANLVYKKPSVLNDVNMHYCPGCSHGVVHKLVAEVI
                     EEMDIQDKTIG"
     gene            complement(12141..13223)
                     /locus_tag="PKH58_06995"
     CDS             complement(12141..13223)
                     /locus_tag="PKH58_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNX88812.1"
                     /translation="MEEIVLMKGNEAIAHAAIRSGCDGYFGYPITPQSEVMETLMELK
                     PWETTGMVVLQAESETASINMVYGAAGCGKRTMTSSSSPGISLMQEGMSYMAGAELPA
                     VVVNVMRGGPGLGTIQPSQADYFQTVKGGGHGDYRVIALAPASVQEMADFTVLAFDLA
                     FKYRVTCMLLADGVIGQMMEKVQLPPFRPRWTEEEIRRQTPWAIMGKTPDRKPNVITS
                     LALQADEMEEINLRLQGRYREIEKNEVRFEEIMCDDAEYLVVAFGISARVSQKAVELA
                     RQEGHKIGLLRPITLYPFPTIEISRLADQVKGMLTVELSAGQMVEDVRLAVNGRIPVE
                     FYGRMGGIVPSPDEVLRALKEKVIRK"
     gene            complement(13321..13557)
                     /locus_tag="PKH58_07000"
     CDS             complement(13321..13557)
                     /locus_tag="PKH58_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX88813.1"
                     /translation="MAKAKGAVVVNTERCKGCNLCVVACPWDVLSLNKEANSKGYNYS
                     YMMSPDDCVGCATCAYICPDACITVYKLKAEKAV"
BASE COUNT         3642 a         2922 c         2615 g         4295 t
ORIGIN      
        1 gtttttcgtt tttctgtaaa agtatttttt tgtcccagtc agcaacattc agcgctttaa
       61 tttcaggtaa atgttcagca gccgtattga aatacatcga aatccggtga ggaactgtgc
      121 gttctgattt tacaagatat tgccgggttg tttcatcttt gaaataatga agtactacat
      181 tatcgggcag gtatctgata gccggaacag acatgattgt atccaccgta acgctgtctt
      241 tccagatagt atcagacctc atgtaatttt cgaaggtagt ggtaaaaaca gattggctgt
      301 aggcaagcgc ctcaccgggc tgatagaaat tatccctatt gccatcagtg agggcataca
      361 ccctgtattt accattccgc acattgggca cagtaaaccg tcccatgttg tcggtacggg
      421 aaattcgcag aaagggcttt ttcataaaaa ccgtatccga caaatcgtcg tagataccta
      481 cagtaacacc cggtacagga ttaagatttt gcgcatcaat cacggttcct gctatctgca
      541 tggtatcaat ttcattgccg gttgaaaaag caaagaagta attcttcagc ggattgcttt
      601 cgttattgtc cacaatagca tcaccaaaat tgatggaata ggtcgtattg ggaattaatg
      661 tatcattgag ctgcaccgta accgtcttcg cgtacgattt tacttctgga gcgatacgct
      721 gcgggggcga aatgataaca ttatcggatg ccttaagtac attgacattc tcatcgaaaa
      781 tgatttccac ttttcctttt gtcacattca gcgatttatt gttgggaaaa gattttacca
      841 cgcttggagg tgtttcgtct ttcggacctc cctgaggacc ttgtccgcga ttggcacagg
      901 catacagggc agcaacagtt acagttgccg caacaaggaa tcttatgttt ctagtatatt
      961 tcttcattgc ttattaatta tcaacacttt atgtcctatc cgacacaaca ggcatttctt
     1021 ttcctcacaa taatttttct tcaactccag aagcgcctgt gagtcgaaag ccgatttaca
     1081 gtttatcccg agtttctccc agcggtgtac tatggagtta ctctcgggtt ttatctcttc
     1141 tagcagacgg atagcctgtt ccgttctgtt ttcgtcattt ttcttttttc cccaggcaaa
     1201 aataaaaggt attacagtat ttatcaataa tatatcaatc gatttattgc ccattatttt
     1261 cctggaaacg cggctttctc gtccaaacag aaaatgtttc tgccagtatt ctgaaacttc
     1321 ggtatcaaaa agttttcgca ggtcctgaca atccggtgtt tcgagaatta atgagaataa
     1381 tttcgaagac cggtgtatca gtgatgcaaa ttgcgccagc cttatcgtgg ggaaattttc
     1441 aggccggagt ctcagcagct tccactgcga aacctcaatg gggttcagtc cgtattttgt
     1501 ctgcaaaaaa cggtattctt tctgtagctc caacggatat ttatcctcaa cgggcttttc
     1561 gagaaatccg gcttgtccca tcaacagtgc ttccagttga aagatgttat ctttatgttt
     1621 ggcaagcaca gcaagcggca gagacctggc catagcttca aacgcatgac tgttggttga
     1681 aaaaccgtag cttcgtgcca gagaaatgta aaaagcgtct tcccagtgat tttgagactg
     1741 tgaaagcatg gtttcgatgg cggcaacctt tctctccagg cgttcggtca gaagagttgt
     1801 cttccaactg ctgataagta tggacggaac ctgatgaatc ttatcttcac aggctatcca
     1861 tttccggctg gtaatcagat cgtcatatcg tgtctgaata tcaaccggta ctgccagctc
     1921 catctggggt attaactcac cgtttgaccg gaacacatca acatcggctt tctgtactac
     1981 atgcaggatg acattgtcat aagctttgtc attctgatga ttatgttttt tccaatcggc
     2041 actctgattg tgtatttcga tattaccagc ccagagcgta tcatcaattt taagttttgc
     2101 attaaagaaa tctggtccgg catcggtatt caactttccc gggtcaatca cctccacact
     2161 atcaccgcgt gttgtagtca gctgcgttgc ctcaaacaac ctgtattgcc atacatagtg
     2221 taaaatcgat tccttcatta ttataaaaaa attttattcc tgtttaacta agaaatctgt
     2281 aacagtaaac aaatttttac ttatcaaccg gatattcttt caacattata accctcgtcc
     2341 aaccagtact gtaacgcttc gggcagtgca ctcaacatgt ttttttctgc tttgagcgaa
     2401 tcatgaaaaa ccacgattga tccgttccgg cttcgccttt tcacttcatt cacaatacgt
     2461 tcaggctcga tatttttatc ataatcatag gtgataaaat cccacatgat tattcgaaaa
     2521 ttctttttta acgctttgat ttgtgaaagt ttaatccgtc cgtgtggtgg ccggaataat
     2581 ttgctgtcga tatactccga tgctttttct atatttttga ggtatacatc atcgggagta
     2641 tcaaaacctt tcaaatgatt gtatgtgtga tttccaactg catgtccacg gcgtttcact
     2701 tcatcgaata cttccgggta acggcttaca ttttcagcta cgcaaaaaaa agtggctttc
     2761 actccgtaac gatcgaggat atccagcacc tgaggggtaa cctgaggatg cgggccatcg
     2821 tcgaaagtaa ggtaaatggc tttcccggct ggattgatcc gccatactac gttcttaaaa
     2881 agcgatttga taatctccgg tacctgaagc attttatatt ctgaaacttc ccgaaacagc
     2941 aaaagatatt gccattacgg gaagtaatat atttattgtg ctgattgata tttgtttgca
     3001 aatgtttcaa aaaccggtag atacttgttg aatatcttct ttccgggagt ttcctgactg
     3061 atctgcaaca cattattcag aatggccagg ttgcgtccta tagagctgcc tgaactattt
     3121 tgctgcgatg gagttaaact cgcataccag gtcaggtatt ccacactggt ttttgccaca
     3181 tcatccagta ttttgttgcc ttcgttaatc aatcccaatt tgaagcaata atgagccagc
     3241 atattggatg aataatcgtg cggtacggta gcagccggga ctactttttc gcagtatttg
     3301 tacgcttttc ttgcacggac tgtatcacct tcgttaacca gcgcttcaat cagataagca
     3361 aacattgccc ggtgtgtacg gcacatacgc atgatattat catcgatata cacttttgga
     3421 ttgtcaattc caccatattt gaacttcgtc atcaggtttt cgtacatttc atccgtgttc
     3481 acattagtaa ctccttcaac tttctgagcc acaggaacca catgataagc cagacctgtg
     3541 cgttcaaagt attttgttac tgaagatgga taatattctt cagcaactgt tactgccaca
     3601 taaataggtc ttttccagtt gttttccttg atcatttcga gcatcatggt ttcgctcttg
     3661 gtcagacgag atggcagatc gaaaaccatt tttggaacaa tttcggcttt tttctttaca
     3721 gcaactgttc cgttcttaac tatttcatca gcattaacgg gcaggaaaag ctgttttgag
     3781 gggaagaaac catcaccttc aattttggta ttcggatctt cgcttttgat aaaatcaaat
     3841 gctgttttca tatccagcga ctgtccctgc aacacatctt ccacctgcat gacttcattt
     3901 ttaccggaga tataatcctt gggttcccac gaaatgggca gcggggtgga tttatacgaa
     3961 gcacgtttca tctgatcaat ataccagtcg gtttgcaggt aactgaggtt acatacccga
     4021 acatcagtac gaacaccctc cacttcctgg ttgtaccaga gcgggaaagt atcgttgtcg
     4081 ccgttggtga agatgatagc atttggtttg caggaattga gataattctg accaaaatcc
     4141 cgggcagtat agcggttgct gcgatcgtga tcatcccagt tttgagcagc catgagcaca
     4201 ggtacaccca gacaggcaac agcggcaagc atagcgctta ctcttttcgg gaggaatctg
     4261 tcgagcaact gcatcacacc gagtactcct aacccgatcc agatactgaa tgcatagaat
     4321 gaacctgcat aggcataatc tctttctcgg ggttgatagg gtgtttggtt cagataaacc
     4381 acaatagcaa gaccagtgag gacaaaaagc aatccggtga tccagaaggt atgattaccc
     4441 tttttacctg aattgagcag gaagaaaaga ccgagtattc ccagtaatag cggcaacata
     4501 aagtagacat tgtgaccttt attttcttta agctcagttg gtagattgct ttgattgcct
     4561 acaagcatat tgtcgacaaa cggtattccg gtaatccagt taccatgttc aatttctcca
     4621 tagctttgaa tatcgttctg acgaccgctg aagttccaca taaaatagcg ccagtacata
     4681 aaattaacct gataatcgag gaagaagttg aggttttcgc caaaagtagg cattacatca
     4741 cttttttcct gaccgcagaa atcgtagctg attttctttc cacttctaac atagttagtt
     4801 gtataatacg aaacatgcga tttgtcgctg ctgtacatac gtgggaacag catcataaaa
     4861 cgactgtcca ttttataatc gctcttataa cccgaaacaa tgtattcatc tttttcagac
     4921 gcgtcttttt tgggttttgg gcaatagatg ggtgttttat cggcaatcgg gacacaggta
     4981 tttccctgaa catcaagttt cagcggagcg ctgaacacag ctccgtaaat gagcggacgg
     5041 tcgccgtact gttcgcggtt gaggtatgat ttaagcgcaa agatgttgtc gggtgagttc
     5101 tgatccatgg taggctgagc agctgagcga atcataatga tggaatagga agaatagcct
     5161 atcagcatca cagcaatcat ggccatactt gtattcaacc agcgggcact tatatttttt
     5221 ttctttcgga agaagaaaaa aaccataccg gcaataatta caattcccaa aaagatattt
     5281 tcaccaatga acggaatacc ggccagcgtt actgccagca tgtatgaaat cgccattctg
     5341 gtgtaattct tttccgtgaa cgattcatag agagaccaga caattgcagc aaccaatatg
     5401 agaatataga catacactcc ggaattgaaa gacataccca gggtattgac aaagaaaagt
     5461 tcaaacttgg aggctacaac cacaaaaccg ggtatcagtc cgtaaagcac cagtcccaaa
     5521 atgaccactg aggtgagcaa tgcaagcatt gaacctttga cattgacctt atactttttg
     5581 aagtaataaa ccagtacaat tgccggaata gccagcaggt tgagcaggtg aactccaatg
     5641 gaaagtccca tgagataggc tatgagaatg agccatttat cggaaccttt gtgatcggca
     5701 acgcgctccc acttgaggat aagccagaaa accaccgcgg tgaaaagtga tgaataagca
     5761 taaacttcac cctcaacggc tgagaaccag aacgtatcgg agaaagtata agccagtgct
     5821 ccaaccatac cggcacctaa aattccgatt atatttgcag ccgagtattc ctcttcacgc
     5881 tttccaatca cctgctgagc caggtgtgtg atggtccaga acaggaatag aatagtaaat
     5941 gcactggcaa ttgccgagaa tgaattcacc atcatggcaa ctttagtagg gtcagaggca
     6001 aaaagtgtaa aaaacttggc tgtaagcata aagaacggcg ctccgggagg atgtcctacg
     6061 tccaacttgt atgctgaaga aataaactca ccgcaatccc agaaactggc tgacggctca
     6121 atagtgagaa gataagtaac tgctgccaca gcaaaagcca cccatccgaa gatattgttc
     6181 aaaaatctga attttttcat cggattctaa ttaatatttg aatacattct tgaatttaca
     6241 cttttaacaa aaaaaacgtt caaagataca aagaaaattc agtttgcagg taaagcaaac
     6301 agcattttag agaatattaa aaaaaatgac agccgcaatc tctgatgaac ggctgtcatg
     6361 taaaaattta ttgatcaggt attagcagag aattattgtt ctgttatctt cacaccggca
     6421 ggaatttcga atttatcagc cggaggtgat actgcttcta ttttggttac ttcaattttt
     6481 gtagggattt ccatggcttt tcccgccatt ttcatcatca ggttaccaca tgcgaggtac
     6541 tccacatcca ttttcagttc gtcgtttttc atccggtatt tcacacaggg atagcccaga
     6601 tatttttctt ctcccacttt ttccagtttc attttaccca tcatttcggt ggtcaggttt
     6661 tccatgtcca cacccagttg gttaatcata actgcccggt tgtagtgaaa accggttttt
     6721 ttgtccatgt ctatttccca gttatcttcc ccctttacta ttttgagcga agttgtggat
     6781 attttctggc caagcatttc catggatgta gtggtttcgg tggtttccca ttcgccccac
     6841 ttatcgaaat aaatcaccgg attgctttct atgcccatta cttctgtttt ctgataaact
     6901 ataccctttt caatcggata ttttttacct ttctttactt gtgcaactga ttctgtggtt
     6961 gctttttcgt ccgtttttgt tttgtcggac tgcttttgtc cgcaagctgc gattaaaaaa
     7021 ccgatacaga gaataaaaat taattttttc atattcataa atgattaata ggtgaaaagt
     7081 attattccaa aactcattga caattttaat taactaatta tttggtattt acagaccaat
     7141 tacattttta ccttcaatat tgactacttc catcagtgta tcttcatttc tacattcttc
     7201 ccgacagcac ttacttttcc ggtcatttca agcttaccac ctacatccat attgtggtta
     7261 tcatatttgg taaaagttaa ccttttttaa atttcatctg aattatcagt ttattgattt
     7321 gagttcaaaa aaattcagaa tagaaaaatg ccaattcccg tcaacttatc cagcagactc
     7381 atgatctgtt tctactgaaa gcgtcttttc aaaatgcatt tcacaggtca ggatggacat
     7441 tttacttcct gaagtttatt tcataaattc agaagcaaag ttttgcatat cgaaattctt
     7501 gaattcaaat aatttctgct ttgaggatag ataacccgcc agcgcctgga gcgaataaat
     7561 acctttagcg ggcatattca gttgtgtatt ggttgccgat gaggtcaact ggtattgaat
     7621 ttcttcggct ctgtcgtaca ccggaatatt cttgatcagg ttttctttgt accagtccag
     7681 ataggcaatc attttcagtg tattttgttt acagtacgtg attttcgtgt cgtgaatttt
     7741 ccgaataact gcattccagt gcttaatatc ttcggatgga ggggcaggac attcgttaca
     7801 ttcgtaaata ctggtgatgg agcgaagttc gtcattcagc ttttgcaggt cagtttccag
     7861 tttttctttt ccggacgtat tttgcagagt agcatagcgg ttggcaatct ctgtttcgaa
     7921 tgaactgagc tgatttctga ctttttcctg ttcctgcaag agagccacct gattcatggc
     7981 tttatcgttg gtggctttta tgtacggatt gtttttattt gcctgagcca tagccatctg
     8041 ttctgcccca taagcctgtg cccaagcttt tcgggaattt tcgtccatat ttttcacatt
     8101 cttcacttcg tccatcgaaa gattgtgttg ctgcagtact ttgtttgcga gtgcttgttt
     8161 atcttcctga gtcatctttt tctttttcga cattgcctcc agttcctgtt gcgaaatacc
     8221 cgattttgca gtgatttctt tcatcataga agctttaatc cgatcttcgt tgccactcat
     8281 gtaatcttcg ttggctcttg atctttttga aatatcttct tcaacaatat catacagact
     8341 actcagggaa gtttcgtagt tttggcggat ttcctgtgag cacttacaca ccgaatcggg
     8401 tgatgctggt acatttttca gatactcgaa tgctgttttt tgcgcagtaa tcgtaccagt
     8461 caatgccatt agtatgagta caaataatac agatgttgtt ttcataggaa taaaattatt
     8521 aaaaggttaa ggataaaaaa tgcctgaagt tttatatcca aaaggattaa taacttcagg
     8581 caacaattgt tattgtttca ggaattctac ccgccggttt tttgccttat tttcgggagt
     8641 atcgttagga gccaccggtt ttgtttcacc ggcaccctcc gttgtaagcc tggaagcatc
     8701 gattccaaac ttcgcattga gttcattttt aaccgattcg gctcttcgtt ttgataagtc
     8761 caaattcctg gcatcatcgc catcgctgtc ggtatgaccg gtaattttca ctttcacgcc
     8821 cggattttca gtcagcacgt cggcaatact tttcagtgtt ccgtaggatt cgggtttaat
     8881 atcggctttg ttcacatcga aggtgattcc gtaagtcacc agttttcctt cggttatcag
     8941 tttgctgcgg gtatcgggtg atgcagtggt gattttaatg ttagttacaa atggtgcact
     9001 gtgccgatcc caaccggaaa ataacaattt attgaatttg gtttcagcgt agatatttgt
     9061 tggaacatcg atcactttct ggccctgatg atatattcta actctgcgtt tttgtatcca
     9121 cacaatgaca tgatttggtt cccctgtaac tacgggtgct tttgaacctt gccctgtaag
     9181 ccagtcttta tctgtatcgt atcctttagt ttcccaaccg tctttcatag gtgaaatgtg
     9241 taatcctctc agaccggggt aaagatcact atttacttct tttgggtttt cggggttttc
     9301 ctgatacagg gtcagacaga taccgtatcc aaaatcatcg tcaggaataa gatcaaattc
     9361 tacgatgaaa ttatccggga atgcaatctc tttggtataa caatacactg catcttcgcc
     9421 attcatatgc aaccattttc cgggtgcttt attcacagtt ttcacttcgc cacttccgtt
     9481 agtagtccac agtgccggaa aatcgccgat tgcatcctga ctgaaatcct cgaaaaaaag
     9541 tattttatcc ccgggtacaa aatcatatgc tgttacagat tcgagtttct gaggactttc
     9601 actctttgct ttaacctttc cgtcggtatt cccaacatct tctgcaactt cttcttcccc
     9661 gttatcatta ttttttgtct gttttttttg tggtttttca acgctatcga gcactttatc
     9721 ggttgcatta tccactttct gatcaattcg attttcgact ttttcaacta cccggttttt
     9781 aacacgctgt cccgctctct cgatccaacc ctgagcagaa gttgggagta aaaaaaacat
     9841 acaaatacat acaagaacta aaattcgttt cattttttga cagtaatttt gagtttatac
     9901 cacatttttc ccggaacaca agttccagaa tattaattcg tattatttaa tttaataaaa
     9961 cacagttgaa gtaataaata ttgaagccag aattgctgag agtacaatag aacctagtat
    10021 tactacaatc aggctaatca caaaataggt gactttttta tcttcaggtg ttttcttgag
    10081 aggttgaaga cctaaaaaaa gcagatataa tccatacaaa ccggctaatg aagccagaat
    10141 tcccaatgtt ggaataatca gcagtatacc tgcaacgagc atgggggtgt atgaatacac
    10201 aaccagtttg aaggattgca gaaagttttt ttcagacccg aaggaggaag ccaataaatc
    10261 aatgatgtaa gcagttaaaa atgcaccacc gatagtgctg accaaactga taagggcata
    10321 acgcaacccg tagccgatgg aaccaacatg aacaccaaaa acattttgtc cgataagtcc
    10381 atagccgata aaactggcta cagcagttat taatgccaat ggcaacaagt agttaattgt
    10441 cagtgttttt acatctgtct gttctgtttc aatgatcggc cattctgttt ttggagccat
    10501 cagaatactt tttacacgat taattaagtt catacatttt atttttttaa tggttttata
    10561 aattaaggaa ttaatattca cgatcttatt tacttttaaa acatcaaaat aaatccgcaa
    10621 ctaaaaaaat tgtatgactg caaccattgt ttcagtatct tgtcgtatag ttcaacacaa
    10681 agctattctt cattttttct gacaaaaaca aaattttcca aatatataac atttttttta
    10741 gataaagaca ttttttaacc caaatatcaa attcaggaac aaaaaaaaag acttacccat
    10801 tcgggcaagt cttcatcttt tttatttgca tttttagtta tacttctgta tcaggtccat
    10861 acctactttt atagcttcct cgttttgagg taaaagatga tggtgccgtt cggggagtga
    10921 ttttttcaat cctttcagga cattctctac tttcactatt ggtctgattt ttaataatcc
    10981 accaagaata atcatattca atgttttcga agcattgttg gcaaatgcat attcagtcgc
    11041 atcaatccga taaacgttga tatctgtccg tgtcggaaat ttgcggaaac cattactgtc
    11101 aaaaatcagt gtacctcccg gtttcacctt actttcaaat ttttcgagcg aaggctgatt
    11161 caatacgatt acagtatcgt agttgttcag aatgggggaa ctgattcttt cgtcgcttag
    11221 tattactgtt acgttggctg taccgccacg ttgttccggt ccgtaagatg gaagccagct
    11281 tacttcctgg ccctgcatca tacctgaata taccagaatt tttcccattg acagtacacc
    11341 ttgtccacca aaaccagata ttaatatttc ttctttcata ttttttatag ctttcagcta
    11401 tcagcagata gcaatgttgt gctttttttt attgaaaatt accggcagca aattgctgac
    11461 agctgattgt ttatttaaat atccttcaaa tctccaagcg gatattttgg aaacatattt
    11521 tccaccatcc aatcgttgga agcttcggga gacagtttcc agccagagtt acaggttgaa
    11581 acgacttcaa caatcgatgt ccctttttta agcatcgaat tttcgaatgc tttccgaagt
    11641 gcttttttgg ttttccgtac gtttgccacg ttgtgtacac tttgacgggt tacatagcaa
    11701 gttccgtcaa gttgagcaag cattggggta atattgaggg gatatccatt gagttccaca
    11761 ttgcgtccat aaggtgtagt ggctgttttc atacccacga gtgtcgtagg tgccatctgg
    11821 ccacctgtca taccgtaaat accattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnccga tagttttgtc ctgaatatcc atttcttcga tcacttcagc caccagcttg
    12001 tgtaccacac catgactgca tcccggacaa tagtgcatgt tgacatcatt cagcaccgat
    12061 ggtttcttat aaaccagatt tgcgggattt attatttgtt ctgtattcat agttactttt
    12121 ttttagctat ggaaaaaagt ttatttccgg attacttttt ctttaagagc cctgagtact
    12181 tcatcgggtg acggtacgat acctcccatc ctgccgtaaa attcaaccgg tattctgccg
    12241 tttacagcca gacgaacatc ttcgaccatc tgtccggcac tcaattcaac ggtaagcatt
    12301 cctttcacct gatcagccag acggctgatt tcaattgtcg ggaatggata aagcgttatc
    12361 gggcgaagca atccgatttt gtgaccttcc tgacgtgcaa gttcgactgc tttctgactt
    12421 acacgggcac tgataccaaa ggctaccacc agatattcgg catcatcaca cattatttct
    12481 tcaaaacgaa cttcgttttt ctcgatttcg cgataacgac cctgaagacg caggttgatt
    12541 tcttccattt catctgcctg caatgccagc gatgtgatta cattcggttt ccggtctggt
    12601 gttttaccca ttatcgccca gggcgtttgc cggcgaattt cttcttcggt ccagcgcgga
    12661 cggaacggag gtaactgcac tttttccatc atctgcccga tcactccatc ggcaagcagc
    12721 atacaggtaa cacgatattt gaatgcaagg tcaaaagcca ggacggtgaa gtctgccatt
    12781 tcctgaacag atgcaggagc aagggcaata acccgataat cgccgtgacc tcctcctttt
    12841 acggtctgaa aatagtcagc ctgactgggt tgtatggttc cgagaccggg accaccgcgc
    12901 atcacattga caaccacagc ggggagctct gctccggcca tataagacat gccttcctgc
    12961 atcagactga tacctgggct tgacgacgat gtcatggttc gtttaccgca accagcagcg
    13021 ccatatacca tattgattga agctgtttca ctttcggcct gtagcaccac cattccggta
    13081 gtttcccagg gtttaagttc catcagcgtt tccataactt ccgactgcgg agtaatcggg
    13141 taaccgaagt agccgtcgca tccgctgcgg atagccgcat gtgcaatagc ttcgttgccc
    13201 ttcattaata cgatttcttc catattgttt ttattttgag atatccgata acattatatt
    13261 tcgggtatcg ctttactact tggtttattg ttcagaccgg ttaacgcggt cagagtcttt
    13321 ttaaactgct ttttcagctt tcagtttata tactgtgatg caagcatcgg ggcatatata
    13381 agcacatgtg gcacaaccca cgcaatcgtc gggcgacatc atatacgaat aattgtatcc
    13441 tttggagttt gcttctttgt tgagcgaaag tacgtcccat ggacatgcca cgacacataa
    13501 attacatcct ttacagcgct cggtgttaac caccaccgca ccttttgcct ttgccattat
    13561 tagttcaatt tatc
//
LOCUS       DAOPYB010000071        13526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5006_length_13526_cov_9.214906, whole genome shotgun sequence.
ACCESSION   DAOPYB010000071 DAOPYB010000000
VERSION     DAOPYB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13526
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5006_length_13526_cov_9.214906"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1200)
                     /locus_tag="PKH58_07005"
     CDS             complement(43..1200)
                     /locus_tag="PKH58_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX88814.1"
                     /translation="MNKVYQTNQIMKIIFVVGAMLIVLASTIFTNRLAGKLSIEEQKK
                     IEIWAEATRQLILADETTDMNFILNIIEGNTTIPVIITDNNDQMIDYRNIKAPEKNNE
                     EFFRKKIATLKNRKNRFEINLKESNTKQYIYYDDSLLLKQLYIFPYIQFGVIFVFFII
                     VILAFSSSKRAEQNKVWVGLSKETAHQLGTPISSLLAWTELLKIRYEDDKLITDMEKD
                     VNRLKVIADRFSKIGSKPDLKKSDLKETLSNAVNYMNNRSSNKVEISLNFNEGIPSII
                     PLNVPLFEWVIENLCKNAIDAMNGIGKIALNVKNTENDIIIDITDNGKGIERNMYKAI
                     FSPGFTTKERGWGLGLSLAKRIIEEYHKGKIFVKHSEINVGTTFRIILPVN"
     gene            1356..2237
                     /gene="era"
                     /locus_tag="PKH58_07010"
     CDS             1356..2237
                     /gene="era"
                     /locus_tag="PKH58_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNX88815.1"
                     /translation="MHKSGFVNIVGNPNVGKSTLMNALVGERLSIITSKAQTTRHRIL
                     GIVNGDDFQIVYSDTPGVLKPNYKLQESMLSFSRSALVDADVILYVTDVFDNYEKNKD
                     FVEKVKMNTAPIILVVNKIDLINQEKLIEMVEKWKSVLPNAEIFPISAKEKFNIEALL
                     KRIKELLPESPPFFDKDQLTDKPARFFVTEIIREKILLNYEKEIPYSVEVEVEQFQEE
                     ENLIRINAVIYAERDSQKGILIGHGGKSLKKVGIEARKDIEGFFEKKVFLELYVKVEK
                     DWRNKDNLLRNFGYQPE"
     gene            2328..2576
                     /locus_tag="PKH58_07015"
     CDS             2328..2576
                     /locus_tag="PKH58_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNX88816.1"
                     /translation="MKTSGGGILAALLGGAVVGAALGLLLAPEKGEDTRAKIVEGFED
                     TKSKVVEALKKRGINLSKSDLEDLVDELKSEADAKTTV"
     gene            2583..2978
                     /locus_tag="PKH58_07020"
     CDS             2583..2978
                     /locus_tag="PKH58_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNX88817.1"
                     /translation="MTNKSGNISNFNQLFTDIKDYVKLQGDYVKIEFVEKITKILSLL
                     LFILIVVVLAMGMLFYLLFSLAYILAPTVGFVISFGIIAGIYLVLLTVVLIFRKSLIV
                     NPLLRLIVAMFYDDAKEDNEIRDGIETDN"
     gene            2956..3228
                     /locus_tag="PKH58_07025"
     CDS             2956..3228
                     /locus_tag="PKH58_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88818.1"
                     /translation="MESRQITDKNITLEDMLVFKKEARQKIEQQKVQIISTAQQLIPF
                     SSSSTTSFAPLTFLTSAFRKGRVMTVIQGAIIGYNFVRKIRKLIKK"
     gene            complement(3305..5803)
                     /locus_tag="PKH58_07030"
     CDS             complement(3305..5803)
                     /locus_tag="PKH58_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNX88819.1"
                     /translation="MTKYFHKIVVSAFLLIGCQFASAVPASPYPVIITQPDGTKLTVY
                     LKGDEYHHYRTTEDGILIIKNNEGTYNYASMDAKGKISDTKIKAQNTKNRTENEKLFV
                     SKLVNSNSLNNTGEKTQIKKVAARKNTVSANVVRSSGYPKIGSPKSLVILVNFADASF
                     VTPNPQSAFYNLLNETGYSTNGGTGSARDYFRDNSMGTFSPDFVVVGPYTLPQNIEYY
                     GKNNADDSDQNPIQMVVDACKLANENGVDFTQFDTDNDNLVDNVFVYYAGYNEAENAP
                     DNTVWPHRWGIYPTAIYSGGNYSGSVYSVTFDGKRIEDYACTSELKGKSGTNMCGIGT
                     FTHEFGHVLGLADMYATDGGTHHTLSSWDIMDYGPYLNSGRTPPNYSAFERFSLGYLT
                     PIEIKTPQNILIDTLASGNQAYLISSSDLPFSMAYSNPTEYFLLENRQKKGWDKYLPG
                     HGLLVTRVKYNQNNWDYNTPNNTASAMGVDIMEADGIATDNTMAGDPFPGTDAIKNYT
                     PKLISGVLMTNKALTDIDETNGVISLKFMGGGNAPLLKTKGLTTQFNTVQGNPTIGQT
                     ISIEGLNLEANIKISFNLGTHFEIKKETDPETDWGKSILLTPVDSVVLPTNIQIRYNP
                     TEPSYNSVHYDILEITTTFANKMTLPLSGKSTRKVYVVPPIAAEAKDVTYKSFVASWN
                     SVFDATGYYLTVYNISDNGLVDTVFSNKWLTSTSDTLYNLISNRDYYYQVQASDKNTL
                     YGYENITSYSNTVKVRSAVYPQKKELRVVPSGNGSVLVFVPSNTSVVNVFNTIGQKIR
                     SFTANSDIPEITNLPRGQIYIIQADEFRTKVLIP"
     gene            complement(5897..8332)
                     /locus_tag="PKH58_07035"
     CDS             complement(5897..8332)
                     /locus_tag="PKH58_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNX88820.1"
                     /translation="MYYPETFEQKISFNIIRQLLKDRCLCPLGEEMVDRMKYTADYNE
                     VLQSLSQTDETLRLITISNEELPVGDFFDIREALQRVQVVGLYLDETEIFNLKRALEA
                     VRRLVLFINRQDTELFPELHKLTAGINSFEAITKRIDAILDKFGKVKDNASPELNRIR
                     RDIFKVQSSISKTLQNILRQAQTDGYVDKDVSPTMREGRLVIPVSPAFKRKVSGIIHD
                     ESATGKTVYIEPAQVVEANNRVRELESEERREVMRILVEFTDFLRPYTDEIMASQHFL
                     GIIDFLRAKSLFAIEIKAIKPRVDNTRQLEWNKAIHPLLYLTLKKQQKTVVPLDITLN
                     EKQRILIISGPNAGGKSVCLKTVALLQYMLQCGMLIPLHENSRTGIFERLFIDIGDEQ
                     SIENDLSTYSSHLLNMKFFIKNSNQNTLLLIDEFGSGTEPQIGGAIAEASLERFNRNG
                     AYGVITTHYTNLKHYAEDTEGIVNGAMLYDRQHLQPLFQLQIGNPGSSFAVEIARKIG
                     LPEDLIADAAQKVGLEHLDYDKHLQDIVRDKRYWERKRMEIRQKEKRLDETLEKYETE
                     LGLTEKKRKEITTQAKQEAENLLKEANAKIENTIRTIKESMADKEKTRNVRTELEKFK
                     EKVTDKKVQEHKPKTKLPFKKNAEKIIDEKEVILHPGSAVRLKGQTATGTIIELNDKH
                     AVVAFGQLKSTVKISNLEAVSNNQMKKEKRLSDGLGTPVTDEVRQRKLTFKSEIDVRG
                     MRGDEALQAVTYFIDDAVMVGVASVRILHGTGTGALRQLIRQYLNTVHGVLRFHDEHV
                     QFGGAGITVVEFE"
     gene            complement(8392..9198)
                     /gene="mazG"
                     /locus_tag="PKH58_07040"
     CDS             complement(8392..9198)
                     /gene="mazG"
                     /locus_tag="PKH58_07040"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNX88821.1"
                     /translation="MNTAYREHTTEEKLQEFERLLNIMTRLRAECPWDKEQTFESLRT
                     NTIEETYELADAILSGDMNEIRKELGDLLLHIVFYAQMGTETNDFDIYDVCKGLSEKL
                     IYRHPHIFGETEAHTSEIVMQNWEKLKLKEKGGNRTVLAGVPDALPALIKAHRMQDKA
                     RSVGFDWEHRDQVWTKVKEEIDEFQTEIERMDKDKMEDEFGDVMFSLINAARLYDINP
                     ENALERTNRKFASRFNYLEAQTIAKGKDLKNMTLAEMDEIWEEAKKKQNG"
     gene            9479..9826
                     /locus_tag="PKH58_07045"
     CDS             9479..9826
                     /locus_tag="PKH58_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88822.1"
                     /translation="MKETKIVYDAVPDYRTNTKQGENQVKLPKLSFCILMDLAGLATY
                     ALPAMGEWGDILWAPISGMIFMKTFGGLTGMIGGIANMAEELIPFTDFIPTFTIGYFY
                     TKFQTSKANKHKD"
     gene            complement(9907..10758)
                     /locus_tag="PKH58_07050"
     CDS             complement(9907..10758)
                     /locus_tag="PKH58_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX88823.1"
                     /translation="MKSFLNQFNISQSVNYTETIGLCLSGGGALGFAHIGVLQALEDY
                     RIIPNMISGTSMGSIIGSLYAAGYTPSQMLKLIDDGRLYRITKLMTFQPAFWKSGLTS
                     QAAIISLMNDLIPNNSFEKLEMPMHVCVSNLTTGKYEIVDRSNKLDIWVSASCSIPGI
                     FNGMKINDMIYVDGGLLNNMPAQPLRNKCSVVIGSDVVPHSSTKKNQKSRDTLVTSLR
                     VGIHQNSIPGREMCDFLIEPKAIEKFHEFSFENFQTIYHYGYKATVEMINENPEILRL
                     QKPIETK"
     gene            10966..13143
                     /locus_tag="PKH58_07055"
     CDS             10966..13143
                     /locus_tag="PKH58_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX88824.1"
                     /translation="MKRTLFFLLLIACIQFTQADLLTDITDGKFRPKSYADMHSLNDG
                     EHYTVMKDNKMIIKYNYKTGKSVDTILDLNTIKKSPLVTISGYTFSPNENKILVYANP
                     KYRYRRTFEANYYVFDVKRNELKPLSENGPQEVPLFSPDSRYIAFARENNLYLHKLDF
                     GTESAITKDGEFGKIINGTPDWVYEEEFTETRYFVFSPDSKLLAFIKFNETDVPTYSM
                     QQYLNSPVVQDELPLYPTVQTFKYPNPGQNISKASVCVYDDFYKSVRTINLDVNEKEF
                     YIPRIKWTADQEKLAIFVMNRNQNRLDMLFANPKSLISKLILSDEDSRYVDYENSDYV
                     QFTADGKYFTFVSEKDGYSHAYLYNINGTLNKQLTKGNWDVTKVYGYNALTQTLYYQS
                     AETSPMQRDIFSVDAKGRKLRLTDGKGMHDAVFSKTYTYFADNASSVNMPTMSILRDN
                     KGIKIRTLEDNAALAAEFKALNLPVKEFFSFTTSEGVRLNGWMLKPVGFSAGKKYPTL
                     MVQYSGPNSQEVLDRWKIDWEYYLSTQGYVVVSVDGRGTGARGADFRKCTYQQLGVLE
                     AKDQIEAARYLGTQSFVDKDRIGIWGWSFGGYMTLLSMSSGEKIFKAGIAVAPVTDWR
                     LYDAVYTERFMRTPQENNRGYDAGSVLNKADKLNGNLLIVHGTADDNVHLPNTMIYLD
                     KLMKAGKHADLQLYTDKNHSILGKETRRHLYQKKFDFLEKNLK"
     gene            complement(13239..>13526)
                     /locus_tag="PKH58_07060"
     CDS             complement(13239..>13526)
                     /locus_tag="PKH58_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX88825.1"
                     /translation="PVFPGGDQGLFGWLSQNIRYPAEAQKKGIQGKVLVEYVIDTDGS
                     IKNARISRSVNELLDKEALRVINAMPNWTPGKHNDKVVKVRYTLPIQFRLQ"
BASE COUNT         3894 a         2549 c         2685 g         4398 t
ORIGIN      
        1 taaaatttat gcaaagataa atcaaataat tgaagaagat tattaattga caggtaaaat
       61 tatcctgaac gttgttccta cgtttatttc agaatgtttt acgaaaattt tacctttatg
      121 atattcttca ataatacgtt tagcaagcga taatcccaac ccccatcctc tttctttggt
      181 agtaaaaccc ggtgaaaaaa tagccttata catatttctt tctattcctt tgccgttgtc
      241 tgtaatatca attattatgt cattttctgt attttttaca ttcagagcga tttttccaat
      301 gccattcata gcgtcaatcg cgtttttaca caggttttca atcacccatt cgaacaaggg
      361 tacattgaga ggaataattg atggaattcc ttcgttaaag ttcagtgata tttctacctt
      421 gttggaactg cggttattca tgtagtttac cgcattcgag agcgtctctt ttaaatctga
      481 tttttttaaa tccggttttg aacctatttt tgaaaaacgg tcggcgatta ctttaagacg
      541 attcacatct ttttccatat cggtgatcag tttgtcatct tcgtatcgta tcttaagaag
      601 ttcagtccat gccagcagag atgaaatggg agtgcccagt tgatgtgctg tttctttaga
      661 gagaccaacc cagactttat tctgttctgc tcgtttggag ctggaaaatg ccagaattac
      721 gatgataaag aatacaaaaa ttaccccaaa ctggatgtat ggaaatatat acagttgttt
      781 aagcagcaaa gaatcatcgt aataaatata ctgttttgta ttcgattctt tgagattgat
      841 ctcaaacctg ttttttctgt ttttgagtgt tgctattttt tttctgaaaa attcttcgtt
      901 gtttttttca ggtgctttta tgtttctgta atctatcatt tggtcattat tatccgtaat
      961 aataaccgga atagtcgtgt tgccttcaat tatattgaga atgaagttca tatcggttgt
     1021 ttcatcggca agtatcagtt gtctggttgc ttcagcccat atttcaattt ttttctgttc
     1081 ttctatagaa agtttacctg ccagccgatt ggtgaaaatt gtagatgcaa ggacaattag
     1141 cattgctccc acaacgaaaa tgattttcat tatctgatta gtttggtaaa ctttattcat
     1201 gacggatgaa aaatttgaac ttaataacga caaaagtaac aattttgtat taaaaacagg
     1261 ctatgaataa agttgaacct gttttattta ttacgaatta tagaattctg ctcttgttgt
     1321 tttgaaaagt attatctttg taaaaaatga taatcatgca taaatccgga tttgttaata
     1381 tcgtgggtaa tcccaatgtg ggaaaatcaa ccttgatgaa tgcccttgtt ggtgaacgtt
     1441 tgtcaattat aacatctaaa gcacaaacta cacgtcatcg tattttaggt attgtgaacg
     1501 gggatgattt tcagattgta tattccgaca caccgggtgt gttgaagcca aactacaagt
     1561 tgcaggagtc tatgttaagt ttttcacgct cggccctggt cgatgctgat gtaatacttt
     1621 acgtaactga tgtgtttgat aattatgaga aaaataaaga ttttgttgaa aaggtgaaga
     1681 tgaatacagc accgataatt cttgttgtaa ataaaataga tttgatcaat caggaaaagc
     1741 tgattgaaat ggttgaaaaa tggaaatcag tgctaccgaa tgcggaaata tttccaattt
     1801 ctgcaaaaga aaagttcaat attgaagcct tattaaaacg tataaaggaa ttattacccg
     1861 aaagtccgcc tttttttgat aaagatcagt tgacggataa accggcaaga ttctttgtaa
     1921 ccgaaattat ccgtgaaaaa atactgctga attatgagaa ggaaattcct tattcggtag
     1981 aagtggaagt agaacagttt caggaagaag aaaatttaat ccggatcaat gctgtaattt
     2041 atgccgaaag agattcgcaa aaaggaattc tgattgggca tgggggaaaa tcgctgaaaa
     2101 aagttggaat tgaagcacgg aaggatatcg aaggcttttt tgaaaagaag gtgtttctgg
     2161 agttatatgt aaaagttgaa aaagactgga gaaataaaga taacttgtta agaaactttg
     2221 gttatcagcc agaataataa attatgttct gaacctttct ttatttgttt ttgttttaat
     2281 attgtaaaga cttaatttta aattttcaaa cattaaaaaa aataattatg aaaacttcag
     2341 gtggtggaat attggctgca ctgctaggtg gtgctgttgt gggtgctgct ttgggtttac
     2401 ttttagcacc cgaaaaaggt gaagatacca gagctaaaat tgtagaaggc ttcgaagata
     2461 ccaagtctaa agtagtggaa gctcttaaga aacgaggaat aaatttaagt aaatcggatc
     2521 tggaagatct tgttgatgaa cttaaatctg aagccgatgc taaaacaaca gtttagtatg
     2581 gtatgacaaa caaatcgggt aacatcagta attttaatca actatttacc gacattaaag
     2641 attatgtgaa gctccagggc gattatgtga aaattgaatt tgtagaaaaa atcacaaaaa
     2701 tcctgtccct tctgcttttc attcttattg tagttgtatt ggccatggga atgctttttt
     2761 atctactgtt ttccctggca tatatcctgg caccgactgt aggatttgta ataagttttg
     2821 gaattattgc cgggatctat cttgtcctgc ttactgtcgt tttgatattt cgtaaatctt
     2881 tgatagttaa tcccctgtta aggttgattg tagctatgtt ttatgatgac gcaaaagaag
     2941 ataatgaaat aagagatgga atcgagacag ataactgata aaaatattac ccttgaagat
     3001 atgctggtat tcaagaaaga agcccggcag aagattgaac aacaaaaagt tcagattata
     3061 tccactgcac agcagttgat acctttctct tcttcatcaa caacttcatt tgctccgtta
     3121 acgtttttga cgtctgcatt tcgcaaaggg agagtgatga ctgtgattca gggggcaatt
     3181 atcgggtata attttgtcag aaaaataaga aaactgatca aaaaataggg tttagatatt
     3241 cgtataacag aaatggcatt attccggtga gtaatgccat ttctgtttaa ttttttacat
     3301 tcaattaagg tatcagtacc tttgttcgga attcatcagc ctgtataatg taaatttgtc
     3361 cgcggggaag atttgtaatt tcaggaatat cagaatttgc cgtaaaactt ctgattttct
     3421 gaccgatggt gttaaataca ttaacgacag aagtattgga aggtacaaac actaaaacag
     3481 atccattgcc tgatggtaca acccttaact cctttttctg cggatataca gcactacgca
     3541 cttttacagt attcgaatat gaagtaatgt tttcatatcc gtacaatgtg ttcttatcac
     3601 tagcctgaac ttggtaataa taatcgcgat tggaaatcag gttatataat gtgtcggaag
     3661 tggaagtgag ccatttattt gagaaaacgg tgtcaaccaa gccattatca gatatattat
     3721 atactgttag ataatatccg gtagcatcga atactgagtt ccagctggca acaaaacttt
     3781 tataagtcac atctttagct tcagctgcaa tcggcggcac aacatatacc ttacgtgtcg
     3841 atttacccga aagcggtaat gtcatcttat tggcgaaagt agtggttatt tcaagaatat
     3901 cgtaatgtac actattatag gatggctctg taggattata acgtatctga atattggttg
     3961 gcaatactac agaatcaacg ggtgttagta atattgactt tccccagtct gtttcagggt
     4021 cagtttcttt tttaatttca aaatgagtac ccaaattaaa tgaaattttt atgtttgctt
     4081 caagatttaa accttctatt gaaatggtct ggccaattgt gggattacct tgtacagtat
     4141 tgaattgagt agttaaacct ttcgttttca acaaaggcgc attgccgcca cccatgaatt
     4201 ttaatgatat tactccgttt gtttcatcaa tatcagttaa tgctttattg gtcattagta
     4261 cacctgaaat caactttggt gtataatttt taattgcatc tgttcccgga aacggatccc
     4321 cggccatcgt attgtctgtt gcaatgccat ctgcttccat aatgtccaca cccattgctg
     4381 aagcagtgtt atttggggtg ttataatccc agttgttctg gttgtattta actcgtgtaa
     4441 ccaacaatcc atgaccgggt aaatatttat cccatccttt tttttgtctg ttttccaaca
     4501 ggaaatactc tgtcggattg gagtatgcca tgctgaaagg taaatctgag gatgaaatta
     4561 agtaagcctg gttaccggat gctaatgtgt ctattaaaat attctgaggt gttttaatct
     4621 caatcggagt taaatatccc aacgaaaacc gttcgaaagc cgaatagttt ggaggagttc
     4681 gtccactgtt cagatagggc ccataatcca ttatatccca gctggataat gtgtggtgtg
     4741 tgcctccatc agtagcatac atatcagcta aacctaaaac gtgtccaaat tcatgtgtaa
     4801 atgttccgat gccacacata ttagttccac ttttaccttt gagctccgaa gtacatgcat
     4861 agtcttctat tcgcttacca tcaaaagtta cagaataaac actccctgaa taatttccgc
     4921 cgctataaat ggcggttgga tagattcccc atcgatgagg ccacacggta ttgtcaggag
     4981 cattttctgc ttcattatat ccggcgtaat aaacaaatac gttatcaacc agattgtcat
     5041 tatcagtgtc aaactgggtg aaatctactc cattctcatt tgcgagttta caggcatcaa
     5101 ccaccatttg gatgggattc tggtcggaat catcagcgtt attttttccg taatactcaa
     5161 tgttctgagg tagcgtataa ggacctacaa ctacaaaatc aggtgaaaaa gtacccatgg
     5221 aattgtccct gaaataatca cgggctgaac cggtgcctcc attagtactg taaccggttt
     5281 cattcagcag attataaaat gcagactgag ggttaggtgt aacaaatgac gcatcggcaa
     5341 agttgacgag tatcaccaat gatttgggtg agccgatttt agggtaaccg gaagaacgaa
     5401 cgacattcgc agaaacagta ttttttctgg cagcaacttt tttaatctgt gttttttcac
     5461 ctgtattatt cagggagttt gaatttacaa gttttgaaac aaataatttt tcgttttcag
     5521 tcctgttttt ggtattttgc gcctttattt tggtgtctga aattttccct ttggcatcca
     5581 tggaggcata gttatatgtg ccttcattgt tttttataat taaaattccg tcttcagtag
     5641 tacggtaatg atgatattca tcgcctttga ggtacacggt taactttgta ccgtctggtt
     5701 gagtaattat aacaggatag ggcgaagctg gtaccgcact tgcaaactga cagccaatta
     5761 aaagaaatgc agatacaaca attttatgaa aatatttagt cattcaatga gtaaagttaa
     5821 ttatgaaagt acaaatataa tgtttttttg atttatagca gaattgaaac tgataataca
     5881 aatgttgaac catttcttat tcaaattcca caactgtaat tccggcacca ccaaattgta
     5941 catgttcatc gtggaagcgg agaacgccgt gtacagtatt cagatattga cgaatgagtt
     6001 gccgaagtgc gcctgtacca gttccatgca ggatgcgaac acttgcaacg cctaccatca
     6061 cggcatcatc aatgaagtag gtcactgctt gcagtgcttc atcgccacgc ataccacgca
     6121 catcaatttc cgatttgaaa gtgagttttc gctgacgaac ttcatcggta acaggtgtac
     6181 ccaatccatc cgataaccgt ttttcttttt tcatctgatt attgctgaca gcttcgaggt
     6241 ttgatatttt aacggtagat tttaactgtc cgaaagcgac aactgcgtgc ttgtcattca
     6301 gttctataat tgttccggtt gcagtctgtc ccttcaaccg aacggcggaa cccgggtgaa
     6361 gaatgacttc tttttcatct atgatttttt cagcattttt tttgaaaggt aattttgttt
     6421 tcggtttgtg ttcctggact ttcttatctg ttactttttc tttaaatttt tccagttcag
     6481 tacggacgtt gcgtgttttc tccttatcgg ccatactttc cttgatggta cggatggtat
     6541 tttctatttt cgcattcgct tctttcagca aattttcagc ttcctgcttc gcctgagtgg
     6601 ttatttcctt acgttttttc tcggtcaacc ccaattcagt ttcgtatttc tccagggttt
     6661 catccaatcg tttttctttt tgcctgattt ccatcctttt acgctcccag taacgtttgt
     6721 cccttacaat atcctgtaag tgcttgtcgt aatccagatg ttccagccct actttttggg
     6781 cggcatcagc aattaaatct tcgggcaatc cgattttccg ggctatttct acggcaaacg
     6841 aacttcccgg attaccaatt tgaagctgaa acaggggttg cagatgctgg cggtcgtaaa
     6901 gcatggctcc gttaacaata ccttcggtat cttcggcgta atgtttcaga ttggtgtagt
     6961 gggtggtaat tactccataa gcaccgttcc ggttgaagcg ttcgagactg gcttcagcaa
     7021 tagcgccacc aatctgaggt tcggtaccgg aaccgaattc atcaatgagc aataaggtat
     7081 tttgattgct gtttttgatg aaaaatttca tatttagaag gtgcgaactg taagtagaaa
     7141 ggtcgttttc gattgattgt tcatcaccta tgtcaataaa taagcgttca aaaatacctg
     7201 tacggctgtt ttcgtgtaaa ggtataagca tcccgcactg aagcatatat tgcagtaatg
     7261 cgactgtttt cagacaaacc gatttacctc ccgcgtttgg tccggatatg atcaggatcc
     7321 gctgtttttc gttaagcgtt atatcgagcg gaactactgt tttttgttgt tttttgagtg
     7381 tcagataaag cagtggatgg attgctttgt tccattcgag ttgccgggta ttatcaaccc
     7441 ggggtttgat agctttgatt tcaatggcaa acaatgattt tgctctcaga aagtcgatga
     7501 tgcccagaaa atgttggctg gccattattt catcggtata gggacggaga aaatcggtaa
     7561 attctaccag aatacgcata acttcgcgtc gttcttcgct ttcgagttca cgcacccggt
     7621 tattggcttc aactacctgt gccggttcaa tataaacggt ttttcccgtt gcactttcat
     7681 catggatgat accgctcacc ttacgtttga atgcgggtga aaccggaata accagtctgc
     7741 cttcacgcat ggtggggctc acatctttgt ctacatatcc atcggtctga gcctggcgca
     7801 gtatgttttg cagagttttc gagatgctgc tttgcacctt gaatatatcc cttctgatcc
     7861 gattaagttc gggagatgca ttatccttca ctttcccgaa tttatccaga atagcatcaa
     7921 tgcgtttggt gatggcttcg aaagaattta tacctgctgt cagcttgtgt aattcgggaa
     7981 aaagttccgt atcctgacgg ttgataaaca gaaccagtct gcgaacggct tcaagcgctc
     8041 gtttcaagtt aaatatttcc gtttcatcca ggtataatcc taccacctga acccgttgaa
     8101 gtgcttcccg gatatcaaaa aaatcaccta ccggcagttc ctcgttactg attgtaatca
     8161 acctgagagt ttcatcggtc tgactgagcg attgaagcac ctcattgtag tcggcggtgt
     8221 acttcatcct gtcgaccatt tcctcgccaa gcggacaaag acatctgtcc ttcagcaatt
     8281 gtctgataat gttgaaagat attttttgtt cgaaagtttc gggataatac ataaatgatt
     8341 tagaatgctc aggtatgatt gatttttttt ctgaaatttt gttttttctg attatccgtt
     8401 ttgtttcttt ttggcttctt cccagatttc gtccatctcg gccaatgtca tgtttttaag
     8461 gtctttgcct ttggctatgg tttgtgcttc caggtaattg aaacgcgagg caaatttgcg
     8521 gtttgtacgc tccagggcat tttcaggatt tatatcatat aatcgggctg cattaatgag
     8581 actgaacatt acgtcgccga attcatcttc cattttgtct ttgtccatgc gttcgatttc
     8641 ggtctgaaac tcatcaattt cttcctttac ttttgtccac acctgatcgc ggtgctccca
     8701 atcgaagcct acgctacggg ctttatcctg catgcggtgt gctttgatga gcgccggtaa
     8761 agcatcggga acacctgcca gtacagtgcg gttgccgcct ttttctttca gtttcagttt
     8821 ttcccagttt tgcattacaa tttcgctggt atgagcttcg gtttcgccga agatatgcgg
     8881 atgacggtag ataagtttct cgctcaatcc tttgcaaaca tcatatatgt cgaaatcatt
     8941 ggtttctgtt cccatctgcg cataaaatac gatatgcagt aaaagatcgc ccaactcctt
     9001 acgaatttca ttcatgtcgc ctgacagaat tgcatctgcc agttcgtaag tttcctctat
     9061 agtattggtt ctcaggcttt caaacgtttg ttccttatcc catggacatt cggctctgag
     9121 cctggtcatg atgttaagta gtctttcaaa ctcctgcaat ttttcttcag tagtgtgttc
     9181 tctgtaagct gtattcattt ttctatatat ataaattgca aagttaagaa tatattttta
     9241 atcagattca taaaaataga atgtgcttac cttattacgg aaaataatat atttgaaatt
     9301 tcattctata tttgcaaatc agagcatcca gggtgctata attcaaaggt tcatctgctt
     9361 cattgttttt gggtgataac tttttatttt ttgatattaa cacatttcgg cacggtttat
     9421 gaaagatcat acttgttcaa ttcaaaatca ttttatttaa aattataact ttatcggaat
     9481 gaaagaaacg aaaattgttt acgatgccgt accggattat cggacaaata cgaagcaagg
     9541 agaaaatcaa gtgaagttac ctaaactttc gttttgtatt ttgatggatc ttgccggact
     9601 tgccacctat gcattgcccg ctatgggtga gtggggtgat atactttggg ctccaatatc
     9661 tggtatgatt tttatgaaaa catttggagg attaaccggt atgatcggag ggattgccaa
     9721 catggcggag gaactaattc cctttacgga ctttattcca acttttacca tcggctattt
     9781 ctacacaaaa ttccaaacga gtaaagcaaa taagcacaaa gattaaaatc agattttcaa
     9841 ttatacaaat ggttcacaga atattattcg tgtgaaccat ttgtttttat gatttatgca
     9901 gaaactttac tttgtttcaa tgggtttttg caatctcaga atttcgggat tttcattaat
     9961 catttcaact gtagccttgt aaccatagtg ataaatggtt tgaaaatttt cgaaactgaa
    10021 ttcgtgaaat ttctcgatgg cttttggctc tattaggaag tcgcacattt ctcgtcccgg
    10081 tattgaattc tggtgaatgc ctacccttag tgatgttacc agggtgtcgc gcgatttctg
    10141 attttttttg gtggaagaat gaggaactac atcagatcca atcaccaccg aacatttatt
    10201 tcgtagtggc tgtgcaggca tgttgttaag caaacctcca tcaacataaa tcatatcgtt
    10261 gattttcata ccgttgaaaa tacctggaat actgcacgat gcgctgaccc agatgtcgag
    10321 tttattactt cggtcaacga tttcatattt accggttgtc agattggaaa cacaaacgtg
    10381 catgggcatt tccagtttct caaagctgtt gtttggaatc aggtcattca taagtgaaat
    10441 gattgctgcc tgacttgtaa gacctgattt ccagaatgca ggctggaatg tcataagttt
    10501 tgtaatacgg taaagtctgc cgtcatcgat aagtttcaac atctgcgaag gcgtataacc
    10561 tgcagcataa agtgaaccga ttatacttcc catgctcgtt cctgaaatca tgtttggtat
    10621 tatgcggtag tcttcaagcg cctgtaacac accgatgtgt gcaaatccaa gtgcaccgcc
    10681 tccgctcaga cataatccta ttgtttcagt gtagtttact gactgcgaaa tgttaaattg
    10741 atttaaaaaa gatttcattg tgttgttgtt gtttaagcct gcaaagataa tccaaagata
    10801 tttcatttta tgttaaggga aacaaaaaaa aagtcaaata aaggctggtg aagatatttt
    10861 aaggtatttt tgtaagataa taaaatgcaa ttctttcaat atgcatttta tggtttcaga
    10921 tagataataa ctttcaattc tcgatcaaaa caaaaaaacg aaaatatgaa gcgtaccctt
    10981 ttttttctat tgctaattgc atgcatacaa tttactcagg ctgatctgct cacagatatc
    11041 actgatggta aattcagacc aaaatcctat gccgatatgc attctctgaa tgacggagag
    11101 cattatacag taatgaagga taataaaatg attatcaaat acaattataa aacaggcaaa
    11161 tcggtagata caattctgga cctgaataca ataaaaaaat cccccctggt cactatttcc
    11221 ggatatacgt tcagccccaa tgagaataaa attctggttt atgctaatcc caaatatcgt
    11281 taccggcgta catttgaggc caattactat gtgtttgatg tgaaacgcaa cgaactgaaa
    11341 ccgttgtcgg agaacggacc gcaggaagtg ccgcttttct caccagatag caggtacatt
    11401 gcctttgcac gggagaataa tctctacctc cataaactgg attttggaac cgaaagcgcc
    11461 attaccaaag atggagaatt tggtaaaata ataaacggaa cacccgactg ggtttacgaa
    11521 gaagaattta ccgaaacacg atattttgtt ttcagcccag acagcaaact gttggcattc
    11581 attaagttta acgaaacgga cgttccgacc tatagcatgc agcagtatct caatagtccg
    11641 gtagttcagg atgagcttcc gctttatccg actgttcaga ccttcaaata tccaaatccg
    11701 ggccaaaata tttctaaagc cagtgtgtgc gtgtatgacg acttctacaa atcggtcaga
    11761 acgattaatc ttgatgttaa cgaaaaagag ttttatatcc cgcgcatcaa atggactgcc
    11821 gatcaggaaa agttggcgat attcgtgatg aacagaaatc agaatcgtct tgatatgctt
    11881 tttgccaatc cgaaatcgtt gatttctaaa ctcatcctgt ctgacgaaga cagtcggtat
    11941 gtggattatg aaaattccga ttatgttcag ttcacagctg atggtaagta ttttaccttt
    12001 gtgagtgaaa aggatggcta cagtcacgca tatctgtata atattaatgg cacgctcaat
    12061 aaacaactaa caaagggcaa ctgggatgtt acaaaagttt acggatataa tgccctaacc
    12121 caaactcttt attatcagtc ggccgaaact tcacccatgc aacgcgacat tttttcagtt
    12181 gatgccaaag gtcggaaact tcgcctgacc gacggaaaag gaatgcacga tgctgttttt
    12241 tccaaaacat atacatattt tgccgacaat gcctccagtg tgaatatgcc aacgatgagt
    12301 atcctgcgcg acaataaagg aataaaaatt cgtacgctgg aagataatgc tgcgctagct
    12361 gccgaattta aagcgcttaa tctgcccgtt aaggaatttt ttagctttac tacttccgaa
    12421 ggtgtgagac tgaatggctg gatgcttaaa ccggttggct tctcagcggg gaagaaatac
    12481 cccacgctga tggtgcagta tagcggaccc aattcgcagg aagtacttga ccgctggaaa
    12541 attgactggg aatactatct gagtacacaa ggttatgtag tggtaagtgt ggatgggaga
    12601 ggaacgggag ctcgcggagc cgacttccga aaatgtacct atcagcaact gggtgtactc
    12661 gaagctaaag accagataga agcagcccga tacctgggca ctcaatcttt tgtggataaa
    12721 gaccggatcg gcatatgggg atggagtttc ggaggatata tgaccctgct ttccatgagt
    12781 tcgggcgaaa agatattcaa agcaggtatt gctgtagctc ccgttaccga ctggcgtctt
    12841 tacgatgctg tttataccga gcgatttatg cgtaccccgc aggaaaataa ccgtggttac
    12901 gatgcaggtt cggtactcaa taaagctgat aaactgaatg gaaaccttct catcgttcat
    12961 ggcacagctg atgacaacgt gcatttgccc aacaccatga tttatctcga taagcttatg
    13021 aaagccggaa aacatgccga tttgcaactc tatactgaca aaaatcacag tattctgggc
    13081 aaggaaaccc gccggcacct gtatcagaag aaatttgact ttctggaaaa gaacctgaaa
    13141 taatttccac ctgcattaac caaaaagaaa caaaacaata acgccggaat ttcaattccg
    13201 gcgttattgt tttatttaca aggtcaataa agcctctttt attgcaatct gaactgaatt
    13261 ggtaaggtgt atcttacttt caccacttta tcattgtgtt tgccgggagt ccagttgggc
    13321 attgcattaa taacccgtaa cgcttcttta tccagcaatt cgttaacgct ccgtgaaatt
    13381 cttgcatttt ttatgcttcc atcggtgtca attacatatt ctaccagaac ctttccctgg
    13441 ataccctttt tttgagcttc agcaggatat ctgatgtttt gtgaaagcca gccaaacagc
    13501 ccctgatctc cacccggaaa gacagg
//
LOCUS       DAOPYB010000072        13312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5123_length_13312_cov_11.182771, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000072 DAOPYB010000000
VERSION     DAOPYB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13312
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5123_length_13312_cov_11.182771"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..1900
                     /locus_tag="PKH58_07065"
     CDS             254..1900
                     /locus_tag="PKH58_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNX88826.1"
                     /translation="MVSVDGLAVEFGGSTLFSDVSFVINPKDRIALMGKNGAGKSTLL
                     KILAGVREPTRGQVTTPKDFTIAYLPQHIKTNDSRTVFEETAQAYARLHELENRIAYI
                     NEQLTSRTDYESDEYMQLIEEVSALSEKFYSIEETNIDEHIEKTLLGLGFEREDFDRN
                     TSEFSGGWRMRIELAKILLQNPDVILLDEPTNHLDIDSVQWFEDFLINTAKAVVLISH
                     DRAFVDNVTTRTIEVTMGRIYDYKATYSHYLELRKERREQQQKQYDEQQKMIAETQEF
                     IERFKGTYSKTLQVQSRVKMLEKLQIIEVDEQDTSALRLKFPPSPRSGNYPVIAEGVG
                     KAYGEKRVFANADFTIERGQKVAFVGRNGEGKSTMVKCIMGEIEHEGKLTIGHNVKIG
                     YFAQNQASLLDEELTVFQTIDDIAVGDIRTKIRDLLGAFMFGRDDVDKKVKILSGGER
                     TRLAMIRLLLEPVNLLILDEPTNHLDMRTKDILKQALTDFDGTLIVVSHDRDFLDGLV
                     TKVYEFGHHRVTEHLEDIYGFLAKKKMESLREIESRTNQK"
     gene            2038..4476
                     /locus_tag="PKH58_07070"
     CDS             2038..4476
                     /locus_tag="PKH58_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX88827.1"
                     /translation="MVVGQVSFGKPEKINQNWRFVLQDVKDGQEKNINDTRWQKIDLP
                     HDWSVKGQLSPTLASCTGYLPGGIGWYRKNLNIPKDKENEKVYLYFEGVYNRSEVFVN
                     GHSVGKRPNGYISFLYDVTPYVVFGEDNVIAVRVDHSRSADSRWYTGSGIYRNVWVVY
                     SNPVHFAQWGVYAYPTRNKNQHSLNVEVEVENTSSQARNIQIVNELLDKNQKVVARSS
                     KKIQTPANQSVKLTEILNVKSPVLWSLENPCLYELKTTLLLDGKEIDTSVTNTGFRYF
                     TFDPDHGFSLNDTRMKLKGVCLHHDAGVLGSAVYRDVWKRRLLNLKEIGVNAIRTSHN
                     PQSPDIYQLCDELGLLVINEAFDEWEFPKRKWLEGWNKGTPGFQGSFDFFEEWGEKDL
                     ADMVRRDRNHLSVFAWSIGNEVDYPNDPYSHPALNGSKSGFTQPIYGGYKKNAPDAMR
                     LGVIAKKLVAAVKKYDKSRPVTAGLAGVAMSNETEYPGALDIAGYNYTESMYDTDHKK
                     YPRRVIFGSENRHELSAWKSVRDKEFIFGQFLWTGIDYLGESGSWPSRGFYAGLLDFG
                     GFIKPRGYYRQSLWMDKPMAYLGTYPTPVRGSRSQMKDVWSQLDAENGGNYEEKVPSM
                     DAWPIWNYQKGQMIRVVCYTNAPKARLLLNEKEVGKTQLYDDNTGIIFWDIPYEAGTL
                     TVIGLDKNDNEISRNQIQSSKQPYALKIISADRNIAKDQVAQITVQVVDENGIPVMLS
                     DNEITCRIAGEGQLLGLEAGDNEDMSDYTDNKQRVYHGRILAYIKPTNDKGSIKVRFT
                     SPWLETAETDILIK"
     gene            4705..4890
                     /locus_tag="PKH58_07075"
     CDS             4705..4890
                     /locus_tag="PKH58_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88828.1"
                     /translation="MTKDCKRNNGPSGAVYGLGFIGAAIYFISHAATFWAGVLGFLKA
                     IVWPALLVYEAFKSFAM"
     gene            5047..5955
                     /locus_tag="PKH58_07080"
     CDS             5047..5955
                     /locus_tag="PKH58_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX88829.1"
                     /translation="MSHQNASVKSILFALLANLGIAITKTVAAVLTGSGSMLAESIHS
                     YADCGNQGLLFLGLKKSKKAPEEEHPLGHGKEIYFWSFIVALMLFSMGGLFSIYEGIH
                     KLGSHEGLKSPVIAVVVLVISMMLEAASLYGCISQINKIRHNMSLWQWLKNTRQSELV
                     VVLGEDVAALFGLSFALIAVILAMITGNPVWDAVGSIGIGTLLIFVSVFLAVKVKGLL
                     VGQSADKATRDEIQKYLQSRPEVEEVLNVITMQMGENIMVSIKARMVKTDTAESLVRN
                     INSCEAGLKASNKAIQWVFFEPDVEK"
     gene            complement(6057..6845)
                     /locus_tag="PKH58_07085"
     CDS             complement(6057..6845)
                     /locus_tag="PKH58_07085"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX88830.1"
                     /translation="MKTSRFILLIPNLVFLFLLISCSGISKTAKTNQTIKFLSYNVRN
                     CKGMDNITNYQRLADAINKAGAACVALQELDSNTLRSNHCSVLDELAKRTNMFPFFHG
                     SISYQGGKYGIGILTKEKPVSVRAFTLPGSEEKRSILIAEMKNYFFCCTHLSLTPEDR
                     AVSIDIINRKLPANSKKPVILAGDLNAEPQTAEIGKLTENWQILSDTTRYTFPADKPD
                     RCIDYFLFKKDPKFRAQIIGTCVMDEPLASDHRPLLVEIIFLRN"
     gene            6997..7737
                     /locus_tag="PKH58_07090"
     CDS             6997..7737
                     /locus_tag="PKH58_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNX88831.1"
                     /translation="MKAMIFAAGLGTRLRPFTDNMPKALVPVNGRPLLEHIILKLKDA
                     GFQEIIVNVHHFPDMIIGFLKTNDNFGMRIEISDEREMLLDTGGAIKKAAHFFDDGKP
                     FLVHNVDILSNLDLKEVCCQQVADPGRLATLVVSQRDTYRYLLFDKQNYLCGWINEKT
                     GETKPGTFPDLKIYNKLAYAGIQVISPAVFQLMAQQEDKFPIMDFYLQHCRTEKISAF
                     IPDDLKMMDMGKTDLLTEGDLFAKQYML"
     gene            7906..8490
                     /locus_tag="PKH58_07095"
     CDS             7906..8490
                     /locus_tag="PKH58_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNX88832.1"
                     /translation="MKTFDLSGEVRSELGKKATKAVRAAETIPCVLYGGKENVHFVVT
                     ESAVRKLVYSPDVFVVNLTVGSTATKAIMKEIQFHPVSDKILHIDFVEINEAKPVVVE
                     VPVLLKGLAEGVKAGGKLSLEIRKLKVKGLYTNVPEKIEVDVTSVGLGKTVQVGKLHV
                     ENLEILNNKNAVVAQVKLTRAARGAAEAAANASK"
     gene            8503..9066
                     /gene="pth"
                     /locus_tag="PKH58_07100"
     CDS             8503..9066
                     /gene="pth"
                     /locus_tag="PKH58_07100"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNX88833.1"
                     /translation="MKYLIVGLGNIGPEYQDTRHNIGFTILDAFAKASNVFFADNRYG
                     ATTEVKLKGRTLVLLKPSTFMNLSGNALRYWMQKENIPAERVLVLVDDLALAFGTLRL
                     KPKGSDAGHNGLKNIQEVLGHSNYARLRFGIGNGYPRGGQVDYVLGRWTDEQQAALPQ
                     RVETAMDMIRSFCLSGVELTMTQYNNK"
     gene            9141..9542
                     /locus_tag="PKH58_07105"
     CDS             9141..9542
                     /locus_tag="PKH58_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNX88834.1"
                     /translation="MEVRIDKWLWAVRLYKTRSLATEACKKGKVLVQNTAVKPSRMVK
                     PGEVIQIRENPVIYSFKVIALAQNRMNAKLVPDFMENVTTPDQLQLIELARLAAQSGR
                     ARGTGRPTKKERRDLDDFVEPFFLDDSDDED"
     gene            9561..10175
                     /locus_tag="PKH58_07110"
     CDS             9561..10175
                     /locus_tag="PKH58_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNX88835.1"
                     /translation="MKPVLIKKLIFTGTLLAGVLSCIHPIYPEEQLLQHLGTLVLLVP
                     VCIDLKKNRLSLFAFACVSLFIIFHIIGARYIYSFVPYSEWIKSVFQVDINTLFPAAR
                     NNYDRFVHFIFGVLFLPFFVEIIRIKSVRNKILSVLIAWAFIQALSMLYEVFEWMLTL
                     VMSNEAANNYNGQQGDMWDAQKDMAIAMLGSTLMAFCYLFRKAK"
     gene            10190..10747
                     /locus_tag="PKH58_07115"
     CDS             10190..10747
                     /locus_tag="PKH58_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HNX88836.1"
                     /translation="MFIARKLKKENICEYLLYMWQIEDLIRAYNLDIDKINEQIVKPY
                     PVDDTQKKELYDWYESLIDMMRMENVQKSGHIQLNKNQIIQLNDFHQLLLKSGVDAGY
                     AAKFYHILPILNGIRTQQPDKDISDMELCFNFLYGIIVLRMKKADISPETLRAQTEIS
                     KFMILLNKNYISYIQGKLKLEADEM"
     gene            10823..11692
                     /gene="rfbA"
                     /locus_tag="PKH58_07120"
     CDS             10823..11692
                     /gene="rfbA"
                     /locus_tag="PKH58_07120"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNX88837.1"
                     /translation="MKGIILAGGSGTRLYPVTKSISKQIIPVYDKPMIYYPLSTLMLA
                     GIREILIISTPQDIVLYENLLGNGEDIGLKLEYAIQPSPDGLAQAFIIGEKFIGNDPV
                     CMTLGDNIFYGFDFSRTLREAAETKDGAVVFGYYVNDPERYGVAEFDQNGQVISLEEK
                     PKEPKSNYAVTGLYFYGNDVVEKAKGLKPSKRGELEITDLNRLYLEENRLSLKLMGRG
                     MAWLDTGTHDSLLEASNFIATIENRQGLQVACLEEIAYRNGYITRDQLLKLAEPLKKN
                     NYGQYMIKIANEL"
     gene            11728..12276
                     /gene="rfbC"
                     /locus_tag="PKH58_07125"
     CDS             11728..12276
                     /gene="rfbC"
                     /locus_tag="PKH58_07125"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNX88838.1"
                     /translation="MEIIKTPIDDLIIIKPRIFEDARGYFCESYSEKCFREAGIDIHF
                     CQDNQSKSAYGVVRGLHYQLSPVSQSKLVTVIVGSVWDVAVDLRKKSPTFGQWYGVEL
                     TAENHLQFLVPQGFAHGFSVLSETAVFSYKCDNFYSPTQERGIAFDDPQLNIDWKIPH
                     ENALISEKDKKHPSFAQAEMNF"
     gene            12280..13137
                     /gene="rfbD"
                     /locus_tag="PKH58_07130"
     CDS             12280..13137
                     /gene="rfbD"
                     /locus_tag="PKH58_07130"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNX88839.1"
                     /translation="MKQVLITGSNGQLGSEIRVASPLFPQFRFFFTDVQELDICDKDA
                     VSAFVTENKIEAIVNCAAYTAVDKAEDDSETCYKINRDAVEILAEVAREFKLQIIHVS
                     TDYVFDGKSYLPYSEEMAVSPSTVYGKSKLDGETVLTEICPDAVIVRTSWLYSSFGNN
                     FVKTMLRLGREKENLTVIFDQIGTPTYAADLAEAILSILAFDNPQPGIYHYSNEGVCS
                     WYDFTRSIHRFAGITCNLTPIESKEYPVRTPRPHFSVLNKAKIKQTFGIAIPHWEDSL
                     EKCIRLLIS"
BASE COUNT         3953 a         2639 c         3076 g         3644 t
ORIGIN      
        1 tttatcaatt ccgggaccat ttcccgggca ggttctttca acgatgccgt cagtctgctc
       61 ctgcagggta aaagagaaag ctgaagaaaa cgtcagtctc gctgccacga ccgccttgtg
      121 atttgctttg aagataaaaa tatattcaaa atgtcgcact tgtagtgcag aagtaaataa
      181 tcactccttt ccttttttaa ccgttgaaaa ttactcctat ctttgtcatt aaaaaaaaat
      241 ataagacgat tatatggttt ctgtggatgg attggcggta gagtttggcg gttctacgct
      301 tttttcggat gtttcttttg tgataaaccc caaggacaga attgctctga tgggtaaaaa
      361 tggtgcaggt aaatcgactc tgctgaaaat tcttgccggt gttcgtgaac ctaccagggg
      421 tcaggttacc actccaaagg attttaccat tgcttatctc ccgcagcaca tcaaaaccaa
      481 cgacagcagg actgttttcg aagaaacagc gcaggcttat gccaggcttc acgaactgga
      541 aaacagaata gcatacatca atgagcaact gaccagtcgc acggattatg aatcggatga
      601 gtatatgcag ctgattgagg aagtttcggc actgagcgag aaattttaca gcatcgagga
      661 aaccaatatc gatgagcaca ttgagaaaac cctgcttggt ctgggtttcg aaagggaaga
      721 ttttgatcgg aatacgtccg aattcagtgg aggctggcgc atgagaattg aattggcaaa
      781 aatcctgctg caaaatccgg atgtgatcct gctcgatgaa ccaaccaatc acctggatat
      841 cgattcggtg cagtggtttg aggattttct gattaatacg gcaaaagctg tggtgcttat
      901 ttcgcacgat cgtgcatttg tggataatgt tactacccgg accattgaag tgacgatggg
      961 acgtatttac gattataaag cgacctactc ccattacctg gaactccgaa aggaacgtcg
     1021 cgagcaacaa caaaagcagt acgacgaaca gcagaagatg atcgccgaaa cgcaggagtt
     1081 tattgaacgt ttcaaaggga cgtattccaa aacccttcag gtgcagtcgc gggtaaaaat
     1141 gctcgaaaag ctgcaaatca tcgaagtgga cgaacaggat acctccgcgc ttcgattgaa
     1201 atttccgcct tcgccgcggt cgggaaatta tccggtgata gccgaagggg ttgggaaagc
     1261 atacggagaa aaacgggtat ttgcaaacgc tgattttacc atcgaacgtg gacagaaagt
     1321 ggctttcgtg ggtcgcaacg gcgaaggaaa atcgaccatg gtcaaatgca tcatgggtga
     1381 aatagaacat gaaggaaaac tgacaatcgg acataatgtg aaaatcggtt actttgccca
     1441 aaatcaggca tcgctgcttg acgaagagtt gactgtgttt cagactatcg atgatattgc
     1501 cgtaggtgac atccgtacaa aaattcgtga tttactgggt gcgttcatgt ttggacgtga
     1561 tgatgtggac aagaaagtga agatcctttc gggaggtgaa cgaacccggc tggctatgat
     1621 acgcctgctg ctcgaaccgg tcaacctgct catcctcgac gaaccgacaa atcacctgga
     1681 tatgcgcaca aaagatattt tgaaacaggc gctgactgac tttgacggta cgcttatcgt
     1741 ggtttcgcac gaccgcgatt ttctcgacgg actggttacg aaagtgtacg aattcgggca
     1801 tcaccgggta accgagcatc tcgaagatat ctacggcttc ctggcaaaaa agaaaatgga
     1861 aagcttgcgc gaaattgaaa gccggaccaa tcagaaataa ttgtaataaa tccctttcag
     1921 gagggttgag ttgataaata aattcccgta aaaaatgtcg ggagattaaa tttttaaaaa
     1981 aatatacctg tgaaaaagac tgctgtattt ttattcatca tttttatttt acctgtaatg
     2041 gtagttggtc aggtttcttt tggaaaacct gagaaaataa atcagaactg gcgctttgtc
     2101 cttcaggatg ttaaggatgg tcaggaaaag aatatcaacg acacgcgctg gcagaaaatt
     2161 gatttgccgc acgactggag tgtgaaagga cagttaagcc ctacacttgc cagttgcaca
     2221 ggttatctgc ccggcggaat aggctggtat cgtaaaaatt taaatattcc gaaagataaa
     2281 gagaacgaaa aggtctatct ttattttgaa ggagtttata accggagcga agtctttgtt
     2341 aatggtcatt ctgttggcaa acgacccaac ggatatattt cattcctgta tgacgtcacg
     2401 ccctatgttg tttttggcga agataatgtc attgctgtca gggttgatca cagccgttcg
     2461 gccgattccc gctggtacac cggttccgga atttaccgga atgtatgggt ggtttactcc
     2521 aatccggtgc attttgcaca gtggggagtg tatgcctatc cgacacggaa caaaaatcag
     2581 cattcgctca atgttgaagt ggaagtggaa aatacaagtt cccaagcccg caacattcag
     2641 attgtgaatg aactgctgga taagaaccag aaagtggttg ccagaagcag taagaaaatt
     2701 cagacaccgg ctaatcagtc ggtaaaactt accgaaattc tgaatgtgaa aagtccggtg
     2761 ctctggagcc tcgagaatcc ctgtttatac gaactgaaaa ccaccttgct gctggacgga
     2821 aaggaaattg acacttctgt aacgaatacc ggtttcagat actttacgtt tgaccccgat
     2881 catggttttt cgctgaacga tacccggatg aaattgaagg gtgtgtgtct gcatcacgat
     2941 gccggtgtac tgggttcggc ggtttacagg gatgtgtgga aacgcaggct gctgaatttg
     3001 aaggaaattg gcgtaaacgc gatccgtaca agtcataatc ctcagtcacc agatatttac
     3061 caactctgtg atgaactggg attacttgtg atcaacgaag cattcgacga atgggagttc
     3121 ccaaagagaa aatggctgga aggctggaac aagggaaccc ccggttttca gggctcgttt
     3181 gatttctttg aagaatgggg cgaaaaagac ctggcagata tggtgagaag agatcgcaat
     3241 catttatcgg tttttgcctg gagtattggc aatgaggtgg actatcccaa cgatccgtac
     3301 tcacatcccg cactcaatgg cagtaagagc ggatttacac aacctattta tggcggatac
     3361 aaaaaaaatg ctcctgatgc catgagatta ggagttattg caaaaaaact cgttgccgca
     3421 gtaaaaaaat acgataaatc ccgacctgtt actgccggac ttgccggtgt cgccatgtcc
     3481 aatgaaactg aatatcccgg agcgctggat atcgccgggt acaactatac ggagagtatg
     3541 tacgataccg atcataagaa atatccccgg agagtaatct ttggaagtga aaacagacat
     3601 gaattgagtg cctggaaatc ggtaagagat aaagaattta ttttcgggca atttctctgg
     3661 acaggcattg attacctggg cgaatcgggc agctggccat caagaggttt ttatgcggga
     3721 ttactcgatt tcggaggttt tatcaaaccc cgcggttatt accgtcaatc tttgtggatg
     3781 gataaaccga tggcctacct gggaacctat ccgacacccg tcagaggcag tcgttcgcag
     3841 atgaaagacg tgtggtcgca gctcgatgct gaaaacggcg gcaattatga ggaaaaagtc
     3901 ccatcgatgg atgcatggcc catatggaat tatcagaaag gtcagatgat ccgggtggtt
     3961 tgttatacca atgcaccgaa agcaagactg ctgctgaatg aaaaagaagt tggcaagaca
     4021 cagctttatg acgacaatac cggcattatt ttctgggata ttccttatga agcgggaacg
     4081 ctgacggtga ttggacttga taaaaatgat aacgaaatca gcagaaacca gattcaatct
     4141 tcgaaacaac cttatgcgct gaaaataatc tcagctgaca ggaatattgc taaagatcag
     4201 gtagcacaaa tcacggttca ggtggtggat gaaaacggca tacccgtaat gttgtctgat
     4261 aacgaaatta cttgccggat tgcaggcgag ggtcagctgc tgggactgga agccggtgat
     4321 aatgaagata tgagtgatta cacggacaac aaacagcgtg tctatcacgg gagaattctg
     4381 gcatatatta aacccacaaa tgacaaaggc agcatcaaag tcagatttac atcaccttgg
     4441 ctcgaaaccg cagaaacgga tattctgata aagtgagccg gtaaaaaagt aaaatggcag
     4501 cactgaaaca ctccggattg ttttgaaacg tatatgattt tgcctgtttc agaacatata
     4561 tacacatccg ggttgtttcc cggtcatttc ttgttgtgta aaattttgaa gaaaatgaaa
     4621 tcatttccgg attgattgtc tatctttggc gaacaattga ttcctgaatt tcagatgttt
     4681 ttcattaata ataacgaata agaaatgaca aaagattgta agagaaacaa cggcccatcg
     4741 ggtgccgttt acggtttagg atttatcggt gcagccattt atttcatttc acacgctgct
     4801 actttttggg cgggtgtact tggatttctg aaagcaatcg tatggccggc gttgctggta
     4861 tatgaagcct ttaaaagttt cgccatgtaa gcaattttca gcagcaacct gttccttaat
     4921 tttttactct gaaaagattt tcagggtaga ttttgatatg cagctcctaa aggattttag
     4981 ctaattctat tcgtgtaaat tcagggaatt accagcactt atcactccaa ttcactttat
     5041 tgcaatatgt cacatcaaaa cgcttcagta aaatcaatac tttttgccct gttggctaat
     5101 ctgggaattg ccattacaaa aactgttgca gcggtactga ccggttccgg ttccatgctg
     5161 gccgaatcta tccattccta tgccgactgt ggtaaccagg gattgctttt tctgggattg
     5221 aaaaaatcga aaaaagctcc tgaagaggaa catccgctcg gacatggaaa ggaaatctat
     5281 ttctggtcgt ttattgttgc cctgatgctg ttcagtatgg gcggcttatt ttcaatctat
     5341 gaaggcatac acaaactcgg ttcacacgaa ggactgaaaa gcccggtaat agccgttgta
     5401 gtgctggtta tcagtatgat gctcgaagct gcatcgctgt atggctgtat ttcccagatc
     5461 aataagatca ggcacaatat gtcactctgg caatggctga agaatacccg tcagagtgag
     5521 ctggtggtgg tgttgggtga ggatgtggca gctttgttcg ggcttagctt tgctcttatt
     5581 gccgtaatac ttgccatgat tacgggcaac cctgtctggg atgctgtggg cagtatcgga
     5641 attggtaccc tgttgatatt tgtttctgtt tttctggctg tcaaggtcaa agggctgctg
     5701 gtgggtcaga gtgccgataa agctacccgt gatgaaattc agaagtattt gcagagcaga
     5761 cccgaagtgg aagaagtact gaatgtaatc accatgcaga tgggtgaaaa catcatggtc
     5821 tcgatcaaag ctcgaatggt aaaaaccgac actgccgaaa gtctggtgcg aaatatcaac
     5881 agttgtgagg cgggtctgaa agcctcgaat aaagctatac agtgggtgtt ttttgaaccc
     5941 gatgtagaaa aatagccgtt tatttattaa caatgaaaaa gagcagccaa ccggacgctc
     6001 tttttttgtg cggcagaatg gcagaatatt aatattcagt taacaggtta ctgcattcag
     6061 tttctgagaa atattatctc caccagtaaa ggtcggtgat cggaagccag cggttcgtcc
     6121 atcacgcatg taccgatgat ttgtgcccgg aatttcgggt cttttttaaa taaaaaataa
     6181 tcaatacagc ggtctggttt atctgcagga aaagtgtatc gggtagtgtc gctcagtatt
     6241 tgccagtttt ctgttagctt accgatttca gcggtttgag gttcggcgtt cagatcaccc
     6301 gccaggatga caggtttttt gctgtttgct ggtagtttcc ggttgataat atcgatggaa
     6361 accgccctgt cttcgggtgt taatgaaaga tgtgtacagc aaaagaaata atttttcatt
     6421 tcagcaatca ggatactgcg tttttcttca cttccgggca gtgtaaatgc ccgtactgat
     6481 acgggctttt cttttgtcag tattccaatc ccgtattttc ctccctgata actgatggaa
     6541 ccatgaaaaa atggaaacat gttggtccgc tttgccagtt cgtcaagtac cgagcagtga
     6601 tttgatctca gcgtattact gtcaagttcc tgaagagcga cacacgctgc ccccgccttg
     6661 tttatcgcat ctgctaaccg ctgatagttg gtgatattgt ccatgccttt gcagttacgg
     6721 acgttgtagc tgagaaattt aatggtttga tttgttttcg ctgtcttaga aatcccggaa
     6781 caggaaatga gtaagaacag aaaaaccaga ttcggaataa ggaggataaa ccgggatgtt
     6841 ttcatttttg gtagtatgtt tttcagtatc aaaggtacgt ttttttacca gcgaataata
     6901 ataatgataa taaaaacttc agaaataaat ttccggcata tagacgggaa aacagaattt
     6961 tattgctaat tttgtatgaa cttgaaaaaa gacattatga aagcaatgat attcgcagcc
     7021 ggacttggca cacgtctccg accattcact gacaatatgc ccaaagcttt ggttcccgta
     7081 aacggaagac ctttgcttga acatattatt ctgaaattga aagatgcagg atttcaagaa
     7141 ataattgtga atgtgcacca tttccccgac atgattatag gctttctgaa aaccaatgac
     7201 aattttggca tgcggattga aatttcggat gagcgcgaaa tgctcctgga tacaggtggc
     7261 gcgataaaaa aagcggcaca ttttttcgat gatggcaaac cgtttctggt tcacaacgtg
     7321 gatattctct ccaatctcga tctgaaagag gtgtgttgcc aacaagtagc agaccctggg
     7381 aggctggcaa ccctggtggt aagccagcgc gatacctatc gctatctgct ttttgataaa
     7441 cagaattacc tctgcggctg gattaatgaa aaaaccggag aaacaaaacc tggtactttt
     7501 ccggatctga aaatctataa caaactggct tatgccggta tacaggtcat atcgccagct
     7561 gtttttcaac ttatggcgca acaggaagat aaatttccca ttatggattt ttatcttcag
     7621 cattgccgga ccgaaaaaat atctgcattt attcccgacg acctaaagat gatggacatg
     7681 ggcaaaaccg atttgcttac ggaaggtgat ctgttcgcaa aacaatatat gctctgaagt
     7741 taatcctgcc ggaaatttaa aaacagcact tgtttgtaca tttaaaagat tctgactacc
     7801 tttgtaccgc ttttttgcaa aagaaagtgt gatgggaaat taagcaacta atcacttaaa
     7861 aactaaatgc ttaaattttg agtaacacaa ttattattta caaaaatgaa aacatttgat
     7921 ttgtcaggcg aagttcgctc tgaattagga aaaaaagcta caaaagcagt tcgtgctgcc
     7981 gaaactatcc cttgtgttct gtacggtgga aaagaaaacg ttcatttcgt tgtaaccgag
     8041 agtgcagtac gtaaactggt ttattctccc gacgtatttg ttgtaaatct gaccgttggc
     8101 agcacagcta ccaaggcaat catgaaagaa atccagtttc atccggtttc tgataaaatc
     8161 cttcacatcg attttgttga aatcaacgaa gcaaaaccgg tggtcgtaga agttcctgtt
     8221 ctgctcaaag gtttagccga aggtgtgaaa gccggtggta aattaagcct cgaaatccgt
     8281 aaactgaaag ttaaaggtct ttataccaat gttcctgaga aaattgaagt ggacgtgacc
     8341 agtgttggtt tgggtaaaac cgttcaggtt ggtaaactac atgttgaaaa tctggaaatc
     8401 ctgaataaca aaaatgctgt tgttgcacag gttaaactga cacgtgctgc tcgtggagct
     8461 gccgaagccg ctgcaaatgc gtcaaaataa tatttttttc gaatgaaata cttaattgtc
     8521 ggactgggta atataggtcc cgaatatcag gatacccgcc acaacatagg attcacaata
     8581 ttggacgctt ttgctaaggc gtccaatgtt ttttttgcag acaaccgtta cggtgcaacc
     8641 accgaggtga agctgaaagg aagaacgctg gttttgctca agccatccac tttcatgaac
     8701 ctgagtggta atgcgctgcg ttactggatg caaaaagaaa atatccctgc agagcgtgtg
     8761 ctggtgctcg tcgatgacct tgccctcgcg tttgggacac ttcgtttgaa gccgaaaggc
     8821 tcggatgccg gacacaacgg actgaaaaat attcaggaag tgctcggaca cagcaattat
     8881 gcccgcctgc gttttggtat cggcaacgga tatccgcgcg ggggtcaggt tgattatgtg
     8941 cttggaaggt ggacagacga acaacaggct gcactgcccc agcgggtaga aacggccatg
     9001 gatatgatcc ggagcttttg tctgtcggga gttgaactga ctatgacaca gtataataat
     9061 aaatgacaaa aatgatgaat tatgaattat gaatttcgca aacctgaaat tcataattta
     9121 taactaataa ttaaaaaacc gtggaagtcc gaatagataa gtggctctgg gctgtcaggt
     9181 tatataaaac ccgcagtctt gcaactgaag cctgtaagaa aggaaaggtg ctggtgcaaa
     9241 atacagccgt aaaaccgtcg cgtatggtaa aaccaggtga agtcattcag atcagggaaa
     9301 atccggtgat ttattctttt aaggtgattg cacttgctca aaaccggatg aatgccaaac
     9361 tggttcccga ttttatggaa aatgtcacca ctccggatca gcttcaactc atcgaacttg
     9421 cccggcttgc agctcaatcg ggcagggcac ggggcacagg ccgcccgact aaaaaggaaa
     9481 gaagagattt ggatgatttt gtcgaacctt tttttcttga cgacagtgat gatgaagatt
     9541 aatctttttc agaatacatt atgaaaccgg tattgataaa aaaacttatt tttaccggta
     9601 ccctgctggc tggtgtttta tcctgcattc atccgatcta tccggaagaa caattactcc
     9661 agcacctggg aactctggtg cttcttgtac cggtttgcat cgatttgaaa aagaacagac
     9721 taagtctttt tgcttttgca tgcgtttcat tattcatcat ttttcatatc atcggagcac
     9781 ggtacattta ttcctttgtt ccttattcag aatggataaa atcggttttt caggtggaca
     9841 tcaatacgtt gtttcctgcc gcccggaata actacgacag gtttgttcat tttattttcg
     9901 gagttctgtt tttacctttc tttgtagaga ttatcagaat caagagtgtc cggaataaaa
     9961 ttttaagtgt gctgattgca tgggctttta tacaggcact gagcatgctt tacgaagtgt
    10021 ttgaatggat gttaacgttg gttatgtcca atgaagctgc aaataattac aacggtcagc
    10081 agggagatat gtgggatgct cagaaggata tggcgattgc catgcttggt tcaaccctga
    10141 tggccttctg ttatttattc aggaaagcaa aataaatctg tttaattgta tgtttatagc
    10201 ccgaaaactc aaaaaagaga atatctgcga atatctgctc tatatgtggc agattgaaga
    10261 tttaatccgc gcctacaatc ttgatatcga taagataaat gaacagattg tgaaacctta
    10321 tcccgttgac gatacccaaa aaaaggaact gtatgactgg tatgaaagcc ttattgatat
    10381 gatgcgcatg gagaatgttc agaaaagtgg gcatattcag ctgaataaaa atcaaatcat
    10441 ccagcttaac gactttcatc agttattgct caaatcgggc gttgatgccg gttatgccgc
    10501 aaaattctat cacatccttc ccatcctgaa cggcatccgc actcaacagc cagacaagga
    10561 tatcagcgat atggaacttt gctttaattt tctgtacggg ataatcgtgt tgcggatgaa
    10621 aaaagcggat atttcacctg aaaccctgcg ggctcagact gaaatcagta aatttatgat
    10681 tttacttaat aaaaactaca tcagctacat tcagggaaaa ttaaaactcg aagcagatga
    10741 gatgtagctt aataattcaa gaaaaattct gataagaaac agaaaaaacg tacctttgtg
    10801 taaactcaaa aaataaaaga ctatgaaagg cattatcctc gccggtggtt cgggtacccg
    10861 tttatatcct gtcaccaaaa gcatttccaa gcaaatcatc cccgtttacg acaaaccgat
    10921 gatctactat ccgttatcga ccctgatgtt ggccggtatc cgggaaattc tgataatctc
    10981 cactcctcag gatattgtac tttatgaaaa cctgcttgga aacggtgaag acattggcct
    11041 caaactggaa tatgccattc agccctcacc ggacggactt gcgcaggctt ttatcatcgg
    11101 tgagaaattt attggtaatg accctgtttg tatgactctg ggtgacaata tcttctacgg
    11161 gtttgatttt tcacgcacgt tgcgcgaagc tgccgaaaca aaagacggtg ccgtggtttt
    11221 cggatattat gttaatgatc cggaacgata cggtgttgca gagtttgacc aaaacggcca
    11281 ggttatcagt ctggaagaaa aaccgaaaga acccaaatcg aattatgccg ttacaggttt
    11341 gtatttttac ggaaatgatg tggttgaaaa agccaaaggt ctgaaaccct cgaaacgtgg
    11401 tgaacttgaa attaccgacc tgaacagact gtatctggaa gaaaatcgtc tgagccttaa
    11461 attgatgggg cgggggatgg cctggttgga caccggaacc catgatagtc tgctcgaagc
    11521 atccaatttc atagccacta tcgaaaatcg tcagggactg caggtggcct gcctggaaga
    11581 aattgcttac cgtaacggat acatcacacg cgatcagctc ctcaaacttg ccgagccgct
    11641 gaaaaagaat aactacgggc aatatatgat caaaattgcc aacgaactat aactgaatta
    11701 ctgaaaaaaa tagcagcttc acgataaatg gaaattatta aaacaccgat tgacgactta
    11761 atcatcatca aaccgcggat ttttgaagat gcccgcggat atttctgcga aagctacagc
    11821 gaaaaatgct ttagggaagc aggtattgat attcatttct gtcaggataa ccagtcgaaa
    11881 tctgcctatg gcgtggtacg cggacttcac tatcaactta gtccggtcag ccagtcgaaa
    11941 cttgtcacgg ttattgtcgg ctctgtatgg gatgtggcag tggatttaag aaaaaaatcc
    12001 ccaacattcg gtcagtggta cggggtggaa cttactgctg aaaatcatct gcagtttctt
    12061 gtcccacagg gatttgcaca tggtttctcc gtattgagtg aaacagcggt tttttcctac
    12121 aaatgcgata atttttactc tcccacgcag gaaaggggca ttgcttttga tgatccgcaa
    12181 ctgaatatcg actggaaaat tccgcatgaa aatgcattga tatccgagaa agacaaaaaa
    12241 cacccttcgt ttgctcaggc agaaatgaat ttttaagaga tgaaacaagt acttatcaca
    12301 ggatctaacg gacagttagg cagtgaaatc agagttgcat cgccattatt cccgcagttc
    12361 agattctttt ttaccgacgt gcaggaactc gacatttgcg acaaagatgc agtcagtgca
    12421 tttgtgaccg aaaacaagat tgaggcaata gtgaattgtg ccgcttatac tgcggttgat
    12481 aaagctgaag acgattccga aacctgctac aaaattaacc gcgacgcagt ggaaatcctg
    12541 gcagaggtag cccgggaatt taaattgcaa attattcatg tttccaccga ttatgttttc
    12601 gacgggaaaa gttatctgcc ctacagcgaa gaaatggctg taagtccctc cactgtttac
    12661 ggaaaatcca agctggatgg tgaaacggtt ttaacagaaa tatgtcccga tgcagtaatc
    12721 gtgcgtacat cctggcttta ctcttctttt ggcaacaatt ttgtgaaaac catgctccgg
    12781 ctgggccggg aaaaggaaaa tctgactgta atttttgatc agattggtac gccaacctat
    12841 gcagccgatc tggcagaagc tatactcagc atactggctt tcgataatcc gcaaccggga
    12901 atatatcatt actccaacga gggcgtttgc agttggtatg actttacacg aagtattcac
    12961 cgatttgccg gtattacctg caacctgacc cctattgagt cgaaagagta tccggtgcga
    13021 acaccacgtc cgcacttcag cgtgctgaat aaggcaaaaa ttaaacagac ctttggaatt
    13081 gccattcctc actgggaaga cagtctggaa aaatgtatcc gcttgttgat aagctaattc
    13141 tttgaatgtg tttttctgtt tcagtaatga tgataaattt tgtagaagca ataattcatg
    13201 caaactacag gaataaaccg gaaacgataa aatactggct gaaataacca gtataactca
    13261 aaaaaaatga tttgatcagt aagtcaaatc atttttttta tacctatgta tg
//
LOCUS       DAOPYB010000073        13176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5202_length_13176_cov_8.223992, whole genome shotgun sequence.
ACCESSION   DAOPYB010000073 DAOPYB010000000
VERSION     DAOPYB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5202_length_13176_cov_8.223992"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..1558
                     /gene="miaB"
                     /locus_tag="PKH58_07135"
     CDS             11..1558
                     /gene="miaB"
                     /locus_tag="PKH58_07135"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNX88840.1"
                     /translation="MNVYLETYGCQMNVSDSEVALSILRAAGYERCKEIADADVILIN
                     TCSVRDNAELRVLGRLDVFMQEKKKRKQKSKKSKAPDGSEKRGPVIGVLGCMGQRLKE
                     KLLENPAVDFVAGPDTYRNLPDVIKAIIDNGEKISATNLSPEETYADINPMRTDTNGV
                     STFISITRGCNNMCSYCIVPYTRGRERSRDPQSIVHEAEQLFKQGYKEITLLGQNVDS
                     YQWTNPNDPIDTTCFYQLLELVALIDPKLRVRFQTNHPKDIRKGVLYTMAMYSNICNH
                     IHLPVQSGSNAMLQKMNRKYTREEYLQKIADIREVLPDCAITTDIIAGFCGETEEDHK
                     QTLSLLEEVGFDGAFMFQYSQRPNTLAARKFKDDVSTAEKTKRLNEIIELQSSFSLMS
                     NKKAVGETYEVLVEGFSKRSDKQMTGRTQQNKMCVFDAPQTTANSKKGTAADIKPGDY
                     VDVKINSCTSATLIGEIVQKPKRQYITTAVYDDILDIRNSIRELREKFEKEFLENGEN
                     KKKENKK"
     gene            1592..1999
                     /locus_tag="PKH58_07140"
     CDS             1592..1999
                     /locus_tag="PKH58_07140"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX88841.1"
                     /translation="MTKMKTIYKGNLRSEVTHELSGAKIFTDAPLDNHGNGESFSPTD
                     LLASALGCCMLTIMGVSAQSYGFNIDGTTIETEKIMGTAPRRVIELKLNITFPKGNNY
                     TDQQKRLIDSAARTCPVANSINPEIKKTITFNY"
     gene            2112..2195
                     /locus_tag="PKH58_07145"
     tRNA            2112..2195
                     /locus_tag="PKH58_07145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2146..2148,aa:Leu,seq:caa)
     assembly_gap    2501..2600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2601..5618)
                     /locus_tag="PKH58_07150"
     CDS             complement(<2601..5618)
                     /locus_tag="PKH58_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX88842.1"
                     /translation="MKMKSSFYVKYKNPLLFLLVLILFGGIFSYIRIKSSLFPTITFP
                     KIKIIADAGQQPVEQMTVAVTRPLENAVKQVPNLQSIRSITSRGSCEISAFMDWNANI
                     DLSQQQIEAHINRVRNQLPADVSIMVEKMNPSILPVMGYSVRSTQMDPIELKQLALYT
                     IKPFLSQVPGVSEIRVIGGQDKEYWAVLNQEAIAHLGMSPSAIADVLNKTNFIKSNGY
                     SSDYKYLYLTITDAQLRSLDELKNIVIKNDGSRITYFSDIADIQIRPAKQYIKVNANG
                     EESILIAVIQQPNANVVELSKAMGKQIKELDKTLPKDVSLKPYYDQATFVGDTIKSVT
                     DALWIGLLFAIIVVILFLKSWKSSLTVLITIPVTLFLTIVVLYSLGQTFNIMTLGAIA
                     ASIGLIIDDAIVIVEQIHRTHEENPGKDSNLLVQAAIDYLFKAIIGSSLSTIVIFLPF
                     ALMSGVAGAYFKVMTNTMIITLICSFFVTWILLPVVYLFLSKIVPEKKIKSHEVKEQK
                     WVGYFIDRPVISLVFIGILILSCLLIVPNLPSGFLPEMDEGSIVLDYTSSPGTSLEET
                     DHELHEVEKIITSIPEVDAYSRRTGTQMGFFITEPNTGDYLINLKKKRNRTTQQVTDE
                     IRKKIEATGLPLTVDFGQVITDNLGDLMSSVQPIEIKIFGNDEKLIQKYSKEVAGVVS
                     KVSGTADVFDGIVIAGPTIQIIPNLSILGQYNISPQDFQYQVQTVLEGNVVGNIFDKQ
                     QFTPIRMLYGNTGNASLNEIENSMISLPNGRLKPIKEFGEVKIITGSAEVKSEDLQTL
                     GIVTARLDNSNLGGTVKEIQKQINKSIKLPQGYNIVYGGAYAEQQQSFNELLMILVLS
                     SLLVFLVILFLYRNIKVALVILLISVLGITGSYILLFITGVPLNVGSYTGLIMMVGII
                     GENAIFTYLQFHQNWLESGNKREALIYAISTRLRPKLMTAICAVTALMPLALGIGAGA
                     QMHKPLAIAVIGGFIIALPLLLVVLPSLLNRIKF"
     gene            complement(5615..6553)
                     /locus_tag="PKH58_07155"
     CDS             complement(5615..6553)
                     /locus_tag="PKH58_07155"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX88843.1"
                     /translation="MKVQCVKIGIISIVLSSFLGCNSKKNNNEAGVYVQPKTPVTVVY
                     PSDSFTISDDIRLNATATYLLKSDVKANTTGYIMSVNIKLADWVKRGQTLFSLQTKEA
                     HSLGNTINTLDPSLKFSGNTVVISPASGYVQMLNHQKGDYVMEGDVLATIADKNSFGF
                     LMDVPYEQNQYLINNKNLTVELPDGRNLVGYVSKVMPTVNIAAQTQEVLVQVKNGGTI
                     PENLIATVKFSKRKQTGLTVPKEAVLTNETQSNFWVMKLINDTVAVKISITKGIEDNK
                     RVQIASGNLTSADRIIVTGNYGLSDTAYVRIQNELK"
     gene            complement(6562..7812)
                     /locus_tag="PKH58_07160"
     CDS             complement(6562..7812)
                     /locus_tag="PKH58_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX88844.1"
                     /translation="MFSFKKVITVLTILSFPMAAFTQSTKTLDYFLASARANSPVLND
                     YNNQRYSLKIDSLKMLADNGLQVTGNVDAMYAPVIKGWGYDNVLSNGQNLNSVIRVNK
                     DLLGRENRNTRLADYSLDIRQLFNQSAVTELRLNRAVAEQYINTYSVQQQYQVSQEII
                     RLLQEEEIILKKLTQNASFKQTDYLSFVVTLQQNQLTSKQLYADWLNNYATLNYLAGM
                     VDTTLQKIAPPLLSNDNFIPFEQSIYAESYLTDSLKLANDTKIIKFDYRPKLSAYADG
                     GYMSSFPAKAYKNLGVSAGLSLTVPIYDGDKRKKSLQQKKLQQETLSWYNEFERKQYE
                     QQTLQIESQISQYKQMIATANEQMKYAQTLIEATLRQLPTGDVKVTDFIVSISNYLNL
                     KSSLIQYETNLYNLYNKLKYITLQ"
     gene            complement(7946..8812)
                     /locus_tag="PKH58_07165"
     CDS             complement(7946..8812)
                     /locus_tag="PKH58_07165"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNX88845.1"
                     /translation="MEKVFLVMGLVATLVFASCNEGKKAPQDVQNAIEKMYPGISKVD
                     WENKENASWEAEFKNNGIETSALFSTDGKLLRLEEEIAYSLFPKLAKDYITKNFPGAN
                     VKEISKITDKNKNVTFQAEVKDKNLIFDTLGNFLQTNNNDENSLPANSAPVSESGKVE
                     ISELPQSVHDFVAKNYVGYTIKEANHDPMCTGEDAIDVAISKARSTTYSLIFTPQGKF
                     IQQEEDVDIKTAPSKITEVIKANFADYTISHQIEKLTLVNKSIQYMVDLSKAGAEKEV
                     IFDLNGNVICEH"
     gene            complement(8915..10171)
                     /locus_tag="PKH58_07170"
     CDS             complement(8915..10171)
                     /locus_tag="PKH58_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX88846.1"
                     /translation="MKLTNHIAIHYIGVTIAVLLMATPITYFVLRAVMINSIDESIEF
                     QREWIEKKLGNASPITFTSFDENITIRPTEDTTLYERHYTEDIYVAQDKEMVPYRIME
                     FNTTTNGTSYFIRIQRSLVENEDLLQAILVMQIFILILLFLALLFINRRLNKKIWIPF
                     YKTLNALSLFRIDRDGPLNLPKSSITELNNLNQSLNTLTSHNARIFKAQKEFTENASH
                     ELQMPLAIMQSKLDLLWQTDSLNQEQVNLLEEMSETSSRMRKLNKSLLLLAKIDNNQF
                     AEKREINVPETTRHILTQHEETFSERNIHVVTLQKASSTVIADEILTVTLITNLITNA
                     LRYTPKDGTLEVQILLNAIRVGNTAVENQPLDRSKLFKRFQKQSHCNSSGTGLGLEIC
                     RRICELNNWQIRYEFLEGKHWFVVEF"
     gene            complement(10171..10845)
                     /locus_tag="PKH58_07175"
     CDS             complement(10171..10845)
                     /locus_tag="PKH58_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX88847.1"
                     /translation="MKILIVEDEEKLSQSIAEYLKHENYVCDVAENFNEAMYKIGGFD
                     YDCILLDISLPDGNGLNILKALKADNKTEGVIIISAKDAIDDKIIGLQLGADDYLAKP
                     FHLSELSARIVSVIRRRRFDGNNTLKFNEFLIDTLAKTVYVNGKLLDLTKTQYDLLLY
                     FVMNKNRVLSKIAIAEHLAGDNTEFFDNFDIVYAHVKNLKKKIAQAGGTDYIKSIYGM
                     GYKFEV"
     gene            complement(10981..>13176)
                     /locus_tag="PKH58_07180"
     CDS             complement(10981..>13176)
                     /locus_tag="PKH58_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNX88848.1"
                     /translation="LVISSYNPETSSVTNTLLRCKQTCDSLRIRNTLVIESMNCKNFG
                     EIFTYQKKFSRILDKYTDINGNLTVSAIVLLGQEVWATYLSQPSDRIMGVPVVCAMIS
                     SNSITLPKEGTDLSTWMPEPVYLKDLKDQKCVLSGTVYNYDIEKNIDIIRNLYPGVKN
                     FALLTDNTFGGVVMQAHVRKVFGRHKDLKLILLDGRTKDSKEIESAIASLSPVNSVLL
                     LGTWRVDKNESYYLPTDVYSLSNANKALPVFSLSSIGMGQWAIGGYFPKYRNQGRDIA
                     QSIVKVLNGGYSEPGIYFDQIPNGVQYDAQAFGKFNIDKRLAEKKSQFINDINYLPER
                     YRRWVDLIAVALAVFLIAFIFILTLLYKTERLNTSLKVLHRDNKLILDNIGEGLIFID
                     AYHNIIWENCSTMKGMEDFSEWKIGTKCFNLKIRNINCEAGCPILTHSFDGPHTSREI
                     IHKEAHTYQMYYNSIYNPDSREYIGTVIRVEDVTDRETQTAELRLAKAAAESADKMKS
                     QFIANVSHEIRTPLNAINGFAELLMDEDVTSEDKKMYKGIMQSNTKQLLNLVNDILSL
                     SKIEAGTLDFTNEYIDLNELVNSSYGEFALDAKAKTGRFSGKNIEMQIDIPAETCKII
                     SDPARLREIFTNLMVNAVKFTKKGHIKVGFREREKDVRCFVEDTGKGIPDDQKQKIFD
                     RFVKLDNYVEGTGLGLSICMQIIKHYNGQMGVDSEVGKGSTFWFILPKQ"
BASE COUNT         3922 a         2649 c         2428 g         4077 t
ORIGIN      
        1 agcgtcaatt atgaatgtat atttagaaac atacgggtgt cagatgaatg tcagcgacag
       61 cgaggttgcc ctttccatcc tgcgcgcagc cggatatgag cgttgcaaag agattgcaga
      121 tgctgatgtt attcttataa acacgtgttc agtaagggat aatgcagagc tgcgcgtgct
      181 tggaagactg gatgtcttta tgcaggagaa gaaaaaaagg aaacagaagt caaagaaatc
      241 taaagctccc gacggctccg aaaaacgtgg tcctgtgatt ggcgttcttg gatgcatggg
      301 ccagagacta aaggaaaaac tgctggaaaa tcctgccgtt gattttgttg cagggccgga
      361 cacatataga aatttgcctg atgtcataaa agccattata gataacggag aaaaaatctc
      421 cgccaccaat ctctccccgg aagaaactta tgcagacatc aatcccatgc ggacagacac
      481 caatggtgta agcacattca tctcaattac aaggggatgc aacaacatgt gctcttactg
      541 cattgttcct tacacacgcg gaagagaaag aagcagagat ccgcaaagca ttgttcatga
      601 agcagaacaa ttattcaaac aaggatacaa agagatcact ctgcttggtc agaatgttga
      661 ttcataccag tggacaaatc caaatgaccc catagacaca acatgttttt accagcttct
      721 ggagcttgtt gcacttatag accctaagtt aagagtacgt tttcagacca atcatcctaa
      781 ggatataaga aaaggcgttc tctatacaat ggccatgtat tcaaatattt gcaatcatat
      841 tcatcttccg gtacaaagcg gaagcaacgc aatgctgcag aagatgaaca gaaaatacac
      901 aagagaagaa taccttcaga aaattgccga tataagagaa gtccttccgg attgtgcaat
      961 cacaacagat attattgcgg ggttctgcgg agagacagag gaagaccaca aacaaacact
     1021 ctctctgctt gaggaggttg gctttgatgg agccttcatg ttccaatatt ctcaaaggcc
     1081 taatacatta gcggccagaa agtttaagga tgacgtatct acagctgaaa agaccaaaag
     1141 actaaatgaa attatagaac tgcaaagttc attctccctt atgtccaata aaaaagctgt
     1201 tggagaaact tatgaagttc ttgtggaggg tttctcaaaa cgctctgata aacagatgac
     1261 aggacgcact cagcaaaaca aaatgtgtgt ttttgacgca cctcagacta cggcaaacag
     1321 caaaaaagga actgccgcag atatcaaacc gggagactat gtagatgtga aaatcaacag
     1381 ctgcactagc gccacattaa ttggagagat tgtacaaaag cctaagagac agtatataac
     1441 aactgcagta tatgatgata tcttggacat tagaaattct ataagagaac tgagggaaaa
     1501 atttgagaag gaatttctgg agaacgggga gaacaaaaag aaagagaata aaaaataaat
     1561 attacagata cttacttaaa gaggattaga tatgactaaa atgaaaacta tctataaggg
     1621 aaatctcaga agcgaggtta ctcatgaact atcgggagct aaaattttca cggatgcccc
     1681 tcttgataac catggcaacg gtgaaagttt ttcccctaca gacttgctgg ccagcgcatt
     1741 aggctgttgt atgttaacca tcatgggtgt ttccgcccag agttacgggt ttaatataga
     1801 cggaacgacc attgaaactg agaagattat ggggacggca ccaagacgcg taattgagct
     1861 taagctaaat attacattcc caaaaggcaa taattacaca gaccagcaaa agcgcttaat
     1921 tgattctgct gcaagaactt gccctgttgc aaacagcatt aatccggaga taaaaaagac
     1981 aatcacgttt aactattaat aaatggttag tctttgaaaa gactataaaa aggggggatt
     2041 tttcagtctc ccttttttta ttacctttgc accctgtttg agacaaccac cttgcttttg
     2101 cacggtaaaa tgcccaggtg gcggaattgg tagacgcgct ggtctcaaac accagtggag
     2161 ttaaatctgt gccggttcga tcccggccct gggtactgaa gcggggatga cataaaaatc
     2221 ttccccgttt tctttttcaa cctttcacaa cttattgaaa tcctgataga taatatcaaa
     2281 aactttattg cattttgtag ttcgatattt tttccgtcta attccaattt ttgaggaaat
     2341 caacaagtac tatttctatt atgttcacaa gcccaataaa ttggcagata tagttaatat
     2401 gtgttgagat tttatgttgg gcataatagc agattaatct gattcaaact ttatcctgtt
     2461 taataaactc ggcaatacca ccagcaataa cggcaatgca nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn aaactttatc ctgtttaata aactcggcaa taccaccagc
     2641 aataacggca atgcaataat gaaaccgccg ataacggcaa tggccagtgg cttgtgcatt
     2701 tgcgctcctg cgcctatacc caatgccaaa ggcatcagag cggtaacagc acaaatggca
     2761 gtcatcagtt tggggcgcag tcgtgtgctg atggcataaa tcagtgcttc acgcttgttc
     2821 ccgctttcaa gccaattttg atgaaattgc agataagtga aaatggcatt ctcacctata
     2881 attcccacca tcatgattaa tcccgtgtaa ctgcccacat taagcggtac gcccgtaata
     2941 aagagtaaaa tatagctgcc cgtaattccc aaaacggaaa taagcaaaat cacgagcgcc
     3001 acttttatat ttctgtaaag aaataatata actaaaaaaa caagcaaact cgacaacacc
     3061 aaaatcatta gcagctcgtt gaacgattgc tgctgttcag cataagcgcc accatacacg
     3121 atgttgtatc cctgcggtag tttaatactt ttgttgattt gtttttgaat ttcctttact
     3181 gttccaccta agttactatt atccaaacgt gcggtgacga tgccaagtgt ttgcaaatct
     3241 tcgcttttta cctcagctga cccagttata attttcacct ctccaaattc ttttattggt
     3301 ttcaaccgtc cgtttggaag cgaaatcata ctgttctcaa tttcattgag tgacgcattt
     3361 ccagtatttc catatagcat cctgatgggt gtaaattgct gtttatcaaa tatatttcct
     3421 actacattgc cttcgagtac ggtttgtacc tggtactgga aatcctgagg agatatgttg
     3481 tattgaccca aaatagaaag atttggaatg atttgaatag ttggcccggc aatcactata
     3541 ccatcgaaaa catcagcggt tccggaaact ttactcacca cccctgctac ttctttcgag
     3601 tatttttgta tcaacttttc atcgtttcca aaaattttaa tttcgatcgg ttgcacacta
     3661 ctcatcaagt caccaagatt atcggtgatt acttgcccga aatcaacagt caacggcaac
     3721 ccggtagctt caattttttt acggatttca tcggtaactt gttgggtggt gcgatttcgt
     3781 ttctttttca ggtttattag ataatcaccg gtattgggtt cggtaatgaa aaaacccatc
     3841 tgtgtgcctg ttctacggct gtaggcatcc acttcaggaa ttgaagtaat gattttttcc
     3901 acttcatgca gttcatggtc tgtctcttct agcgacgttc cgggtgacga agtatagtca
     3961 agcacgatac ttccttcatc catttccggc aaaaatcccg aaggtaaatt cggtacaatc
     4021 aataaacagg aaagaatcaa tattccaatg aatacaagcg aaataacagg acgatcgatg
     4081 aaatagccca cccatttttg ctctttcact tcatgcgatt tgattttttt ctcgggaact
     4141 attttcgaaa ggaaaagata cactacagga agcaaaatcc atgttacaaa gaacgaacat
     4201 ataagcgtga taatcatggt attggtcatt accttgaaat aagcgccggc cacgccgctc
     4261 atcaatgcaa acggtagaaa aataacgata gtgcttaacg atgaacctat tattgctttg
     4321 aaaaggtaat caattgccgc ctgcaccaac aagttgctgt ctttaccggg attttcttca
     4381 tgcgtacggt gaatctgttc cactatcacg atggcatcgt caataatcag cccaatggat
     4441 gcagcaatag ctcccagtgt cataatatta aatgtttgac ccagagaata gagcaccaca
     4501 atcgtaagaa acagcgttac aggtatagta atgagcacgg taagactcga tttccacgat
     4561 ttaaggaaca aaataaccac gataattgca aataacaacc caatccataa tgcatcggta
     4621 acgcttttga tagtgtcacc cacaaaggta gcctggtcgt aatagggttt gagtgaaacg
     4681 tctttcggca aggttttgtc caattctttt atctgtttac ccattgcttt tgaaagttcc
     4741 accacattag cgttgggttg ttgaatcact gcaatcagga tgctttcttc accgttggca
     4801 ttgaccttaa tgtattgttt ggcaggtcgg atttgaatat cggcaatatc cgaaaaatag
     4861 gtaatcctgc ttccgtcgtt tttgataacg atgtttttca gttcgtctag actgcgtaat
     4921 tgtgcatcag taatggttaa gtaaagatac ttgtaatcgg atgaataacc gtttgactta
     4981 atgaaattgg ttttgttcaa tacatcggca attgccgaag gagacattcc caagtgtgct
     5041 atagcttcct gattaagtac cgcccaatat tctttatcct gtccgccaat cacacggatt
     5101 tcactcacac ccggcacttg agatagaaac ggcttgatgg tataaagtgc aagttgcttt
     5161 agttcaatgg gatccatttg cgtagaacgt accgaatatc ccataacagg cagaatggac
     5221 gggttcattt tttctaccat aatactcaca tcagcaggca gttgattgcg aaccctattg
     5281 atatgtgctt ctatttgctg ttggcttaaa tcaatgttgg cattccaatc cataaaagcc
     5341 gagatttcac aactgccacg gctcgtaatg cttcttatcg actgcaaatt gggtacctgc
     5401 tttacagcat tttccagcgg tcgtgttacc gctacagtca tttgctcaac gggttgctgc
     5461 ccagcgtcgg caatgatttt gattttcggg aatgtaatcg taggaaatag tgatgatttt
     5521 attctaatat acgaaaatat gccaccgaac aaaatcagca caagtaaaaa tagcaacgga
     5581 tttttgtatt tgacgtagaa ggatgacttc attttcattt cagctcattt tgaattctta
     5641 cgtacgccgt gtcgctcaag ccataatttc ctgtgacaat aatccggtcg gctgaagtga
     5701 gattgcccga tgctatctgt acccttttat tgtcttcaat tcctttagta atcgaaattt
     5761 taacggcaac agtgtcatta atgagtttca ttacccaaaa attggattgc gtttcgttgg
     5821 tcagtacggc ctcttttgga acggtcagcc cggtttgttt tcgcttcgaa aatttgacgg
     5881 ttgcaatcag attctcagga atggttccgc cattttttac ctgtaccagt acttcctggg
     5941 tttgcgctgc gatattaacc gtaggcataa ctttactcac atatcccacc agattgcgtc
     6001 catcgggaag ttccaccgtc aggtttttat tgtttattaa atactgattt tgttcgtaag
     6061 gaacatccat caaaaatcca aaactgtttt tatcagcaat agttgccagc acgtcgcctt
     6121 ccatcacgta atcgcccttc tgatgattga gcatctgcac gtagcccgat gcaggactga
     6181 taacaactgt gttaccagaa aatttaagtg aggggtccag cgtgttaatg gtattcccca
     6241 atgagtgcgc ttccttagtt tgaagcgaaa acagcgtctg tccccgtttt acccaatcag
     6301 caagtttaat atttacagac ataatgtagc ccgttgtatt ggcttttacg tccgatttca
     6361 gcaagtacgt tgccgtagca ttcaagcgaa tatcatcgct gattgtaaaa ctatccgaag
     6421 gatacaccac agttactggc gttttgggct gaacgtaaac tccggcttcg ttgttatttt
     6481 ttttagaatt acatcccaaa aatgaagata aaactattga aataattcct attttcacac
     6541 attgcacttt catcttattt tctattgtaa agtaatgtac tttaatttat tataaagatt
     6601 gtagaggttg gtttcgtatt gaatgagcga tgatttcaaa ttcagataat tactaatcga
     6661 gactataaaa tcagttactt ttacatctcc cgtaggaagt tgtctcaatg tggcttctat
     6721 taaggtttgg gcatatttca tttgctcatt agccgtagct atcatttgtt tgtattgcga
     6781 aatttggctt tcaatttgaa gggtttgctg ttcgtattgt ttcctttcaa actcgttgta
     6841 ccaactaagg gtttcctgtt gcaatttttt ctgttgcagg gatttttttc gcttatcacc
     6901 gtcgtaaatt ggcacggtaa gcgataatcc ggcacttaca cccagatttt tatatgcttt
     6961 ggccggaaat gacgacatat aaccgccatc tgcatacgcc gacaatttcg ggcgataatc
     7021 aaatttaata attttggtat cgtttgctaa tttcagacta tcagtcagat agctttcggc
     7081 gtagatactc tgttcgaaag gaataaaatt atcattggaa agcaaaggcg gtgcaatttt
     7141 ctgtaaagtg gtgtccacca ttccagcaag gtagttcagt gtggcataat tattcagcca
     7201 atcggcgtac aattgtttgg atgtaagttg attttgttgc aacgtgacca caaaactcaa
     7261 ataatcggtt tgcttaaatg atgcgttttg tgtcaatttt ttcaaaataa tttcttcctc
     7321 ctgcaataac cggataatct cctgtgaaac ctgatactgt tgttgaacgg aatacgtgtt
     7381 gatgtactgc tcggcaacgg cacggttcag tcgcagttcc gtaacggcag attggttgaa
     7441 caattgcctg atgtccagcg aataatcagc taaccgggta tttcggtttt cacgccccag
     7501 caagtctttg tttacacgga taacagaatt cagattttgc ccgtttgaga gtacattgtc
     7561 atatccccac ccttttatca ccggcgcgta catggcatcc acatttccgg taacctgtag
     7621 tccgttatcg gcaagcattt tcagactgtc aattttcaac gaataacgct gattattgta
     7681 gtcgtttaac accggactgt tggcacgtgc agaagcgaga aaataatcga gcgttttggt
     7741 ggattgggta aaagccgcca taggaaacga caagattgtt aaaacggtta tgactttttt
     7801 gaaagaaaac atatttttta tactagttga ttttctgaac tttaggctta aataaagaaa
     7861 gaaaaacacg aattggataa gaaattacaa acaaaaatcc gtaaaatcag ccaatacatg
     7921 ttttctacct tgcatatcac attttttaat gttcacaaat tacatttccg tttaggtcaa
     7981 aaataacttc tttctcggca cctgcttttg aaaggtcaac catatactga atggatttat
     8041 tgacaagtgt cagtttttca atctggtgag aaatagtgta atctgcaaaa ttagccttta
     8101 tcacttcagt gattttagaa ggagccgttt ttatatctac atcttcttcc tgctgaataa
     8161 acttgccttg aggcgtaaaa atcagggagt aagttgtact tctggctttt gaaatagcaa
     8221 catcaatggc atcttcgcct gtgcacatcg ggtcatgatt tgcctcttta atagtatatc
     8281 cgacatagtt ttttgctaca aagtcatgaa cgctctgcgg taattcgcta atttccactt
     8341 ttccagattc agaaaccgga gcggaattag ctggtaaaga attctcgtca ttattatttg
     8401 tttgcaaaaa attacccaga gtgtcaaaaa tcaaattttt atctttcact tccgcctgaa
     8461 aagttacatt cttattctta tcagtaattt ttgaaatttc tttcacatta gcaccaggaa
     8521 aatttttagt aatataatct tttgccagtt taggaaataa cgaataagcg atttcttctt
     8581 ctaaacgcag gagttttccg tcagtactga atagagccga cgtttcaata ccattgtttt
     8641 taaattcagc ttcccagctg gcgttttctt tattctccca atctactttt gaaattcctg
     8701 gatacatctt ttcaatagca ttttggacat cttggggcgc ttttttgcct tcattgcaac
     8761 ttgcaaatac aagtgttgca accaaaccca ttactaaaaa tactttttcc attttcgttt
     8821 aatttttcaa ttgattaagt actgcaaagt ataaaaccga atctgaacga aatttgaatt
     8881 tatgataaaa atatttttaa aagtgtaagt aatgttaaaa ttctaccaca aaccaatgtt
     8941 ttccttctaa aaactcatag cggatttgcc aattattcaa ttcacatatt cgtcggcaga
     9001 tttccaagcc taagcccgta ccggatgagt tacaatgtga ttgtttttga aaacgtttga
     9061 aaagcttgga tctgtcaaga ggttgatttt caacagctgt gttgcccact ctgatggcat
     9121 tgagtaaaat ttggacctcc agtgtgccat ctttgggtgt atagcgaaga gcgtttgtaa
     9181 ttagattggt aataagcgtg accgtcagta tttcatcggc aataactgtg gatgaggctt
     9241 tctgtaatgt aacaacatga atattcctct cactaaaagt ttcttcatgc tgggtaagaa
     9301 tgtgtcttgt cgtttcagga acattaattt ctcgcttctc tgcgaattgg ttattatcaa
     9361 tttttgccaa aagcagaagt gatttgttta atttccgcat tctgctacta gtttcggaca
     9421 tttcttccaa aagatttacc tgttcctgat taagtgagtc tgtctgccaa agtaagtcca
     9481 gcttgctctg catgatagcg agtggcatct gtaattcatg cgaagcattt tcagtaaatt
     9541 ctttttgagc tttgaaaatt cgggcattgt ggctggttaa tgtattgagc gattgattta
     9601 gattgtttag ttctgtaatg gatgattttg gtaggtttaa aggtccatct ctatcaatcc
     9661 ggaataaact taatgcattc aaggttttat aaaacggtat ccagattttt ttgttaagcc
     9721 ttctattgat aaacagtaaa gccagaaaca gcaatatcaa aataaaaatt tgcataacaa
     9781 gaatagcctg aagcaaatct tcgttttcta ccaatgatct ttgaatgcga atgaaataag
     9841 aagtaccatt tgtagtagta ttaaattcca ttatccgata gggaaccatt tccttatcct
     9901 gagcaacata aatatcttct gtataatgcc gttcatacaa agttgtgtct tctgtgggac
     9961 gtatagtgat attttcatcg aatgaagtaa aagttatcgg ggaagcattt cccagttttt
    10021 tttcaatcca ctcacgctga aactctatac tctcatcaat gctgttaatc attaccgcac
    10081 gaagcacgaa ataagtgatc ggtgtagcca tcagcaacac agcaatggta acacctatat
    10141 agtgtatggc tatgtggtta gtcagcttca ttatacttca aacttgtaac ccatgccgta
    10201 aattgatttg atgtaatcag taccaccagc ttgagctatt ttcttcttaa gatttttaac
    10261 gtgagcatac actatgtcaa agttatcaaa aaattcggtg ttgtcaccag caagatgctc
    10321 tgcgatagca atttttgaaa gaaccctgtt tttgttcatg acaaaataaa gcaacaggtc
    10381 gtactgcgtt ttagtaagat cgagaagttt gccattcaca taaactgttt ttgcaagtgt
    10441 gtctattaaa aattcgttga atttcagtgt gttatttcca tcaaaccgac ggcgacgtat
    10501 cacagatacg attcgtgcac tcagttccga caaatgaaaa ggtttagcaa gataatcatc
    10561 agcgcccagt tgaagtccga taattttatc atctattgca tcttttgcag aaataattat
    10621 cacaccctct gttttgttat ccgccttcaa tgcttttaga atattcaagc cattgccgtc
    10681 aggtaggctg atatccaaca aaatgcagtc ataatcgaat ccgccaattt tatacatagc
    10741 ctcattaaaa ttttccgcaa catcacacac atagttttcg tgctttaaat attcggcaat
    10801 actttggcta agtttctctt catcttcaac aattaggatt ttcatatctg atgatttttg
    10861 ttgtaaaata tcctcgtaaa tctggattca atctggattc acaaacaaaa aaaataatgc
    10921 tttctttacg attaaaaatg cattttcatg tatttcaatt tcggctcggt gtacaaaaaa
    10981 ttattgtttt ggcaaaatga accagaaagt ggagcctttg ccaacttctg aatctacccc
    11041 catctgacca ttgtaatgtt tgataatctg catgcagata gagagaccta atccagtgcc
    11101 ttctacgtag ttgtccagtt ttacaaatct gtcaaatatc ttttgctttt ggtcatcagg
    11161 gattcctttt ccggtatctt caacaaaaca gcggacatct ttttccctct cacggaatcc
    11221 tactttgata tgcccctttt ttgtaaactt aacagcatta accataagat ttgtaaagat
    11281 ttctctcagc cttgcagggt ctgagattat cttgcaagtc tcagcaggaa tgtcaatctg
    11341 catctctata tttttacctg aaaatcttcc ggtctttgct tttgcgtcaa gtgcaaattc
    11401 accataggaa gaattcacaa gctcattcaa atctatatac tcattagtaa agtcaagagt
    11461 tccagcctca atcttagaca aactcaatat atcattaacc aaattcaaaa gttgcttggt
    11521 attagattgc atgatgccct tgtacatctt tttatcttcc gacgtaacat cctcgtccat
    11581 taatagctcc gcaaatccgt taattgcatt caaaggggtc ctaatctcat gactgacatt
    11641 tgcaataaac tggctcttca tcttatctgc agactcagcc gctgcctttg ccaatctaag
    11701 ctcagccgtt tgagtctccc tgtcagttac atcctccact ctgataacgg taccgatata
    11761 ttctctactg tcgggattat aaatagaatt ataatacatc tgataagtgt gggcctcctt
    11821 gtggattatc tctctggatg tatgcggacc gtcaaaggaa tgggtcaaaa ttggacaacc
    11881 cgcttcacaa ttaatatttc ttattttaag gttaaagcac tttgttccta tcttccactc
    11941 tgagaaatcc tccatgcctt tcatagtgct gcaattttcc caaataatat tgtgatacgc
    12001 atctataaat atcaaccctt cacctatgtt gtccaaaatc aacttgttat ctctgtgtaa
    12061 aactttaagg gaagtgttaa gacgctccgt tttataaagc agcgttaaga taaaaatgaa
    12121 tgcgataaga aaaactgcaa gcgcaacggc aattaaatca acccatctgc ggtaacgttc
    12181 cggcaaataa ttaatgtcat ttataaattg tgactttttc tctgcaagcc tcttatctat
    12241 attaaatttc ccaaaagcct gagcatcata ttgtacacca tttggaatct ggtcaaaata
    12301 tatacccggt tcagaatatc cgccgtttaa gacttttaca atactctgtg ctatgtccct
    12361 tccctgattt ctatactttg gaaagtaacc gcctattgcc cattgaccca tgccaataga
    12421 agacaatgaa aatacaggaa gtgccttatt tgcattagac agagagtaga catcggtagg
    12481 caaataataa gactcattct tatccacacg ccatgttccc aacaatagga cgctgtttac
    12541 aggtgacaga gaggctattg cagattctat ctctttactg tcttttgtcc ttccgtcaag
    12601 taaaatcagt tttaaatctt tatgcctccc aaaaacttta cgcacatggg cctgcatgac
    12661 aactcctcca aaagtattat ctgtaagaag agcaaaattt ttaaccccag gataaagatt
    12721 tcttataata tctatattct tctcaatatc ataattatac acagttcccg acagtacgca
    12781 tttctgatcc tttaagtcct taagataaac tggttccggc atccatgtag aaagatctgt
    12841 tccctctttt ggaagtgtta tgctgttaga acttatcatt gcacaaacaa caggaacacc
    12901 cattattctg tctgacggct gagagagata ggtcgcccat acctcctggc ccaacagaac
    12961 aattgcagaa acagtcagat taccgttaat atccgtgtac ttatctagaa ttctactaaa
    13021 ttttttctga tacgtaaaga tctctccaaa atttttgcaa ttcatactct caattaccag
    13081 agtattacga atacgcaaag aatcgcaagt ttgtttgcat cttaacaatg tatttgtaac
    13141 tgaacttgtc tccggattgt aagaagatat aacaag
//
LOCUS       DAOPYB010000074        12917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5350_length_12917_cov_9.351423, whole genome shotgun sequence.
ACCESSION   DAOPYB010000074 DAOPYB010000000
VERSION     DAOPYB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12917
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5350_length_12917_cov_9.351423"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /gene="rplT"
                     /locus_tag="PKH58_07185"
     CDS             <1..320
                     /gene="rplT"
                     /locus_tag="PKH58_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNX88849.1"
                     /translation="KRRKRILSLTRGYFGGRRNVWTVAKNTWEKGLQYAYRDRKNKKR
                     NFRALWIQRINAAARLEGMSYSKLMGALHKSGIEMNRKVLADLAMNHPEAFSAIVKKA
                     KNA"
     gene            complement(424..1449)
                     /locus_tag="PKH58_07190"
     CDS             complement(424..1449)
                     /locus_tag="PKH58_07190"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNX88850.1"
                     /translation="MKIGILSSGGDCPGINATIRGVGKTAINDYGMEVIGIQNGFSGL
                     LYKDISVLTESSLSGILTMGGTILGTAREKEFRKILKSPDKKDQEVIKKSYKELDLDC
                     LVCIGGNGTQKTTWALSKLGLNVIGIPKTIDNDVFGTDITFGFSTAVDIATEAIDRLH
                     STASSHQRVMVIELMGHHAGWITLYAGMAGGADVILLPELGYNMDTIIEVLNNRKKNG
                     KAYSIVAVAEGIEVEGGTDGHHPATYFAREIEKRTGIESRETVLGYIQRGGSPSAHDR
                     NLGTMLGGYAAKLIAEKKFGRMVSVKGQKITDVSLEEVAGKLRIVKPDDPMVLQGKRM
                     GISFGIE"
     gene            complement(1573..3204)
                     /gene="groL"
                     /locus_tag="PKH58_07195"
     CDS             complement(1573..3204)
                     /gene="groL"
                     /locus_tag="PKH58_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNX88851.1"
                     /translation="MAKEILFNIDARDQLKKGVDELANAVKVTLGPKGRNVILEKKFG
                     APHITKDGVTVAKEIELEDSFQNLGAQLVKEVASKTGDDAGDGTTTATVLAQSIISVG
                     LKNVTAGANPMDLKRGIDKAVEAVVEDIKAQSKTVGDNYDKIEQVATVSANNDARIGK
                     LIADAMRKVSKDGVITIEEAKGTQTTIEIVEGMQFDRGYISPYFVTNTEKMAVEMEKP
                     YILIHDKKISNLKELLPVLEPAVQSGRPLLIIAEDVESEALTTLVVNRLRASLKICAV
                     KAPGFGDRRKEMLEDIAILTGGTVISEEKGIKLENATLEMLGTAEKITVDKDNTTIVN
                     GAGDKEAIAVRVNQIKSQIAATTSDYDREKLQERLAKLAGGVAVLYVGAASEVEMKEM
                     KDRVDDALHATRAAIEEGIVPGGGVAYIRAIDSLNKVKADNDDEQTGIEIIKRAIEEP
                     LRQIVTNSGKEGAVVVQKVREGKADFGYNARSDKFENFFEAGVVDPVKVTRVALENAA
                     SIAGMFLTTECVVVEKKEENPAPQMPMGGGMGGMM"
     gene            complement(3328..3597)
                     /locus_tag="PKH58_07200"
     CDS             complement(3328..3597)
                     /locus_tag="PKH58_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNX88852.1"
                     /translation="MNIKPLADRVLIKPAPAEEKTISGIIIPDSAKEKPLKGEVLAVG
                     NGTKDEEMIVAVGNQVLYGKYAGTELEWEGEKYLIMRQSDILAII"
     gene            complement(3735..4631)
                     /locus_tag="PKH58_07205"
     CDS             complement(3735..4631)
                     /locus_tag="PKH58_07205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX88853.1"
                     /translation="MLSILIPVYNYNIVRLVNDLHQLAMETYVDFEILVIEDGSTSFL
                     KENSTVADLKFCRYSALTHNIGRSAIRNRLADEAIYEHLLFIDCDAEVNSTHFIEKYL
                     SFCREEVVVVGGTAYDPENNDPEFSLRLKYGRMREVCSALKREQNGRYSHFSSFNFLT
                     SKSIFYRIRFDESIRGYGHEDTLFGHRIAELKCDMHHIDNALIHKGLDNNKLFIKKTE
                     ESTRNLFLLYKSNNYPFLANQSKLLRTYIRLDQKKLTSILARLYNLLKSAMLKNLCGA
                     DPSLKVYDLYKLLFLAKISHFH"
     gene            complement(4685..5482)
                     /locus_tag="PKH58_07210"
     CDS             complement(4685..5482)
                     /locus_tag="PKH58_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX88854.1"
                     /translation="MRIKKTIPILHLHLVDHCNLNCRGCDNFSPLAPEVFADILTFEN
                     DCARMSKLVGSRVNEIQLLGGEPLLHPQVTEFIRICRKYFPEVPVNIISNGILLPKQP
                     DDFWETCHKLNINIIVTKYPVKIDHNKIEQLAKKHQVRLSYYGNTESIEKNMQLLPLD
                     LDGKQDAQDSFLRCARANRCISLDNGKLYTCSLIPYVKYFNRKFNQDLRISANDYIDI
                     YQAKNMEEIMNFVSKPMPFCRYCNIKGIEDGISFDISKKQITEWTTK"
     gene            complement(5544..6809)
                     /locus_tag="PKH58_07215"
     CDS             complement(5544..6809)
                     /locus_tag="PKH58_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88855.1"
                     /translation="MRLLIFGEAFLSPAYLPRVRYFVSYFSRKGWQVDFVNEKSEYSY
                     DVPEKVSWLTIDYYKNEKGILGRLEWMIKFIMDLSIDFKGRYFYRKSQSFVTEKKYDL
                     IFCSSSFTFPLTTAARMARKLDVPLFVDLRDIAEQSPDDNHFIAHRPPFFFGNILIKI
                     YKRINIKRRNTVLRKAAGVTTVSPWHVKTLSIYNPNTHLIYNGFDEDLFIPEIRTTSL
                     FTISYFGRIYNEKIRNPRLLFVALDELRKKKIISPGNTIVKWFVDEQSIVVVKNIAES
                     YHLSEFIEFHNFTAPENLPAELNNSSLLLVLCNSQNTKNYFGIMTTKFFEALGANRPV
                     ICIPDNNDNLSEVIKNTQCGLVSSDATEVERYLQDKFSEWQQTGLTTGLLSEETRLDF
                     SRKKGAETLEKLFENAIKKRHYESKDINS"
     gene            complement(6822..8144)
                     /locus_tag="PKH58_07220"
     CDS             complement(6822..8144)
                     /locus_tag="PKH58_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX88856.1"
                     /translation="MTAERLSEITDYIIRFLLGSDSNETFSRLIGYTSDKEQFSKYKI
                     VIIPSGFFNPEFYNTEKSLPQLPLTVWEGIPLLFGEPVTEKSDETVIIHADIIASSFF
                     LMSRYEEIINRNIRDSHGRFPGIKSLPFRAGFLQRPVVDEYGRLLRKLLRETGLDISE
                     PEEKFSKIYLTHDVDQLAHYRNIRGIGGAVLRFFKNPAESLKAVESFAGGIINDPWFT
                     FPWLFDLADKLKNKKPEEKIENIVFIKTGGGELMTDKPRHDVRDRDFNTLFKLCEQHN
                     VKIGLHPSYLAGMKPEYIKNEKKVLDETISQNTIYTRNHFLMSREPEDMQTLIEAGLT
                     DDFTMAYADITGFRLGTCRAVKWIDPVKLQLTSLTLHPLTIMDNTLSDERYMNLKTDE
                     AFLFTKTMIDLVSKHNGELVLLWHNTSVEKNNGQYHRDLYRWLINYLK"
     gene            complement(8151..8357)
                     /locus_tag="PKH58_07225"
     CDS             complement(8151..8357)
                     /locus_tag="PKH58_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88857.1"
                     /translation="MNQKLKLFLYGAGVFCIYALLTWILKLVAKKMPEGDAFLGVFTS
                     TDLWLGLAIAVILTYSHYMKMKIK"
     gene            complement(8486..11311)
                     /gene="uvrA"
                     /locus_tag="PKH58_07230"
     CDS             complement(8486..11311)
                     /gene="uvrA"
                     /locus_tag="PKH58_07230"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNX88858.1"
                     /translation="MHLPDTTENVDVTGARVHNLKNIDVTIPRDALTVITGLSGSGKS
                     SLAFDTIFAEGQRRYIETFSAYARSFLGNIERPDVDKITGLSPVISIEQKTTNKNPRS
                     TVGTTTEIYDFLRLLFARTGIAYSYITGEAMIKYTDDQIIDLVLKNYEGKKTLLLAPV
                     VRGRKGHYRDLFEQIRKKGYLHVRVDGEVREITHNMKVDRYKIHDIEIVIDRLLVTDK
                     DRKRLKDSMQKAMQHGNGIIMLLEKDKDEVRYFSRKLMCPTSGISYDDPAPHNFSFNS
                     PHGYCPKCKGLGFINKIDMAKIIPDTSVSIFNGGIAPLGKHKNSTIFWQIEAILAKYD
                     CTLKTPLADVPDDAIDEIMNGTDEQLHIKNESLGNSNYFLSYDGIIKYIEMQQENSAS
                     ATEQKWANQYSRTIKCNECNGQRLRKESLYFRIHDKNIAELAEMDIAELSDWLNKVDP
                     FLNDKQRVIATEILKELRSRLQFLLDVGLNYLALNRSSQSLSGGESQRIRLATQIGSQ
                     LVNVLYILDEPSIGLHQRDNLRLINSLKQLRDTGNSVIVVEHDKDMMLSADYVLDLGP
                     HAGRLGGELVFAGTPAQMLQADTLTAEYLTGKKKVEIPEKIREGNGKFLTLRGARGNN
                     LKNLDVTFPLGKMICVTGVSGSGKSTLINNTLQPIISQHFYRSLQEPLPYDSIEGIEN
                     IDKIVQVDQSPIGRTPRSNPATYTGVFSDIRNLFATLPEAKIRGFKPGRFSFNTAGGR
                     CETCGGNGYKTIEMNFLPDVYVPCENCNGKRYNHETLEVRFKGKSIADVLNMTINQAV
                     EFFENQSYILNKIKTLQDVGLGYIKLGQSSTTLSGGESQRVKLATELSKRDTGKTLYV
                     LDEPTTGLHFEDIRVLLGVLNRLVNRGNTVIIIEHNMDVIKTVDYIIDIGPEGGNGGG
                     RVICSGSPREVAGNPDSVTGKFLALEF"
     gene            11590..>12917
                     /locus_tag="PKH58_07235"
     CDS             11590..>12917
                     /locus_tag="PKH58_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNX88859.1"
                     /translation="MTQIKERLAALRQSMKSAGISACIIPGTDPHAGEYIADYWKERV
                     WITGFTGSAGTVAITFDKAGLWTDSRYFLQGANQLKDTTIDLMKQGLPETPEIIPWLS
                     EELKSGDKVAVNAQMFSVNAYANMKAELKMSGIELVSLDLLKEIWTDRPALPNNPFFV
                     FDVKYSGKTVSEKLVQLRAELHKAHAQVFVMSALDDIAWLFNIRGNDVAFNPVVIAYA
                     LVDEEKAMLFVDMAKCTDASCDYLNGNGVVIANYQDIYTQLRQIPADKVVMYDGNKLN
                     QSLFEALPAECEKRVGMSPVFKLKSLKNEVEIAGIRKAMIKDGVALTKFFIWLEEHVK
                     DGSLTEVSIADKLRGFRAEQSNFIGESFDTIAGYAGHGAIVHYSATPETASTLKPEGI
                     LLLDSGGQYLEGTTDITRTITLGKPTSKQKKDYTLVLKGHIDLAMAKFPV"
BASE COUNT         3591 a         2641 c         2642 g         4043 t
ORIGIN      
        1 gtaaaagaag aaaaagaatt ttaagcctca ccagaggtta tttcggcgga cgccgtaatg
       61 tttggactgt tgccaaaaat acatgggaaa aaggtcttca gtatgcctat cgtgaccgca
      121 aaaacaaaaa acgcaatttc cgcgcattat ggattcagcg tatcaatgct gctgcacgcc
      181 tagaaggtat gtcatattca aaattgatgg gagctttaca taaatcaggt atcgaaatga
      241 accgtaaagt attagccgac ctggcaatga atcatcctga agcattcagt gcaatcgtaa
      301 aaaaagcgaa gaacgcttaa acaatcaagc aattaaaagt tggatatggt ctgactttgt
      361 agataaaaag aaaaccgcaa cctgtaaggg tattgcggtt ttttatttga tggaatttct
      421 gttttattct attccaaatg aaattcccat tctctttccc tgaagaacca taggatcatc
      481 cggtttaact attcgaagtt ttcccgcaac ttcctcgagt gaaacatcgg taattttttg
      541 ccctttaacg gacaccatac gcccgaattt tttctcagcg attaatttgg ctgcataacc
      601 accgagcatg gtacccagat ttcgatcgtg agctgatggt gatccaccac gctgaatgta
      661 gcctaataca gtctcgcggg attcgatacc tgttctcttt tctatctcac gggcaaagta
      721 tgtcgccgga tgatgtccgt ctgttccacc ttccacttct attccttctg ccacggcaac
      781 aatcgaatag gctttcccgt tttttttacg gttattcaga acctcgatga tggtatccat
      841 attgtatcct aactccggta gaagtatgac atcagcaccg cctgccatac ctgcatataa
      901 agtgatccag cctgcgtgat gccccatcag ttcgatgacc atgacacgct gatgcgaact
      961 ggctgtggag tgaagcctgt cgatagcttc tgtagcaata tcaacggctg tactgaaacc
     1021 gaaagtgata tccgtaccaa aaacatcgtt atcaatggtt tttggtatgc cgattacgtt
     1081 caatcccaac ttagacagag cccaggttgt tttctgtgtg ccattgcctc cgatacatac
     1141 cagacaatcc aggtcaagtt ccttgtaact ttttttgatg acttcctggt cttttttgtc
     1201 aggggatttc agaatttttc gaaactcttt ttcacgggct gtacccaaaa tggttcctcc
     1261 catggtgaga ataccggata atgatgattc ggtgagcaca ctgatgtctt tgtacagtaa
     1321 gcccgaaaat ccattctgaa taccgattac ttccatgcca taatcattga ttgcagtttt
     1381 acctacacca cgaatggtag cgtttatccc cggacaatcg ccacctgaag aaaggattcc
     1441 tattttcata tgattagaat ttgataatta caaaggaaag aaaaaagttt gacaataaat
     1501 gatataaata aaaaaatccc acctgtcatt gtcaacaggt gggatagttg aaacatttct
     1561 atgataagga aattacatca ttccgcccat tccgccaccc ataggcattt gcggagcagg
     1621 attttcttct ttcttttcta caacaacaca ttctgtggta aggaacatac ctgcaataga
     1681 tgctgcattt tcgagtgcta cacgggtaac tttaaccggg tcaacaacac ctgcttcgaa
     1741 gaaattctca aatttatcgc ttcgtgcgtt gtaaccgaag tcagctttac cttcgcgaac
     1801 tttctgcaca actacggcac cttctttgcc tgagttggta acaatttgac gcagaggttc
     1861 ttctatggct ctttttatga tttcaatacc ggtttgttcg tcgtcattgt ctgctttcac
     1921 cttgttcagc gaatcaatcg cgcgaatata ggcaacacca ccaccgggaa caatgccttc
     1981 ttcaatggct gcacgggtag catgcaaagc atcgtcgaca cgatctttca tttctttcat
     2041 ttcaacttcc gaggctgcac ctacgtaaag aactgctacg ccaccagcca gtttggccag
     2101 acgttcctga agtttttcac gatcataatc ggaagtagtg gctgctatct gcgacttaat
     2161 ctgattgaca cgtactgcga tagcttcttt atcgccggcg ccgtttacaa tggtcgtatt
     2221 gtctttatca accgtgatct tttcggctgt gccgagcatt tcgagagttg cattttcgag
     2281 tttaatacct ttttcttccg aaattacagt acctcctgtt aggatagcga tgtcttccag
     2341 catttctttt ctgcggtcac caaagccggg agctttaact gcacaaattt tgagagatgc
     2401 acgcagacga ttaacgacta gtgtggtaag agcttcactt tcaacatctt cggcaatgat
     2461 cagtaaagga cgtccggatt gaacagcagg ctccagtacc ggaagaagtt ccttgaggtt
     2521 ggatattttt ttatcgtgga tgaggatgta tggtttttcc atttccacag ccattttttc
     2581 ggtattggtt acgaagtagg gggaaatgta accacgatcg aactgcatac cttctacaat
     2641 ttcgatggtg gtttgggtac cttttgcttc ttcgatagtg attactccat ctttggatac
     2701 cttacgcata gcatcagcta tcagtttacc gatgcgtgca tcgttattag ccgatacggt
     2761 ggcaacctgt tcgattttat cgtagttatc gcccactgtt ttagactgag ctttgatatc
     2821 ttctactaca gcttctacag ctttgtcaat accacgtttc agatccattg gattggctcc
     2881 ggctgtaaca tttttcagac caacactgat aattgattga gccagaacgg tagctgtggt
     2941 tgtaccatct ccggcatcgt caccggtttt tgaagcaact tccttcacta actgtgctcc
     3001 taaattttga aatgaatctt cgagttcaat ttcttttgca acagtcacac catcttttgt
     3061 tatgtgaggt gcgccaaatt tcttttccag aattacgtta cgaccttttg ggccaagtgt
     3121 aactttcact gcgtttgcca gttcgtcaac accttttttc agttgatcgc gggcatcaat
     3181 attaaataat atttctttag ccatagataa gccccccaac cccctgaagg gggagaaccg
     3241 cgggaggtac ggattttatg aattaattat tattgtttta ttgcttaaat atttgctctt
     3301 tagttccccc ttaaggggag caattgtcta aattattgcc agaatatcgg actgacgcat
     3361 gataaggtac ttttcacctt cccattcgag ttcggttccg gcgtattttc cataaagaac
     3421 ctgattacca actgctacta tcatttcttc atcttttgta ccgtttccaa ctgccagaac
     3481 ttcacctttc agtggctttt cttttgctga atcgggaata attattccgc ttattgtttt
     3541 ttcttctgcc ggtgccggtt tgattagtac ccggtctgct aatggtttaa tgttcataag
     3601 tgtaatattt aatagttaaa tttatatgtt tgttttcttt caataagcga attttgtgcc
     3661 agaaggttaa aactgaaatt ttggcagata atattgttga aatttgaaaa gtcaggaaaa
     3721 atactgccaa cttgtcagtg aaaatgtgaa attttggcaa gaaaaagtag cttgtataaa
     3781 tcataaacct taagtgatgg atcagcaccg cataagtttt ttaacatggc agatttgagc
     3841 agattataca gccgggcgag tattgaggta agtttttttt gatcaagccg gatataagta
     3901 cgcaataatt ttgactggtt tgccaggaat ggatagttat tacttttata aagcagaaaa
     3961 aggtttcggg tgctttcttc tgtctttttg ataaatagct tgttgttgtc cagacctttg
     4021 tgaatgaggg cgttgtcgat gtgatgcatg tcgcacttga gttcggcaat tcggtgtccg
     4081 aaaagagtat cctcgtgacc atatccgcgg atgctttcat caaaacggat tcggtaaaat
     4141 atcgattttg aagtcagaaa attaaatgaa gaaaaatgtg aatatctacc gttttgttca
     4201 cgtttaagtg cggaacatac ttcccgcatt cgcccgtatt tcaggcgtaa gctaaattcg
     4261 gggtcattat tctcgggatc ataagcagta ccgccaacaa ccaccacttc ttcgcgacaa
     4321 aaagacaggt atttttcaat aaaatgcgtt gagtttacct cagcatcgca atcgataaaa
     4381 agtaaatgct cataaatggc ttcgtcggct aaacggttcc ggatagctga gcggccgatg
     4441 ttatgtgtca gtgctgaata tctgcagaat ttcaggtcag caacagtgct gttttctttc
     4501 aggaaagagg tagaaccatc ttcgattacc agaatttcaa aatccacata cgtttccata
     4561 gccaactggt gaaggtcatt caccaatcgg acaatattgt agttgtaaac gggtatgagg
     4621 atggaaagca ttttttttgg ttgtcggttt tattgattta ttgttttggg attgtaatta
     4681 gttgctattt tgttgtccat tcggtaattt gtttttttga aatatcaaac gaaattccgt
     4741 cttcaatccc tttgatgttg caatacctac aaaatggcat gggtttgctg acaaaattca
     4801 taatttcctc catattttta gcctgataaa tatctatgta atcgttggcc gatattctca
     4861 aatcctgatt aaatttccgg ttgaaatact tgacataagg aatcaacgag caggtataaa
     4921 gtttcccgtt atcgagcgat atgcaccgat tggcacgtgc gcatcgcagg aaactgtcct
     4981 gagcatcttg tttgccatcc agatcaagcg gaagcaactg catgtttttc tcaatggatt
     5041 ctgtgtttcc gtagtatgag aggcgaacct gatgtttttt tgcaagttgt tctatcttat
     5101 tgtgatcaat ttttaccgga tatttagtaa caatgatgtt aatattgagt ttatgacagg
     5161 tttcccagaa atcatcgggt tgctttggca gcaaaattcc gttggaaatt atatttacag
     5221 gtacttcagg aaaatatttg cgacagattc tgataaattc tgttacttga ggatgtaaaa
     5281 gtggctcgcc accaagcagt tgtatttcgt tcacacgact accaacgagt ttggacatgc
     5341 gggcacagtc gttttcgaat gtgaggatat cagcaaaaac ttccggggca agtggcgaaa
     5401 aattatcaca cccacgacaa ttcaaattac agtggtctac taaatgcaga tgaagtattg
     5461 gaatagtttt ctttatccgc atgttttttt ttgatattct cctccagaca gggagtaaat
     5521 tgcgacgaaa cagaattatt tttttaggaa ttaatatctt tggattcata atgtcttttt
     5581 tttattgcat tttcaaatag cttttcgagc gtttctgctc ctttttttct ggaaaagtct
     5641 aatcttgttt cctcactaag tagtccggta gttagccccg tttgctgcca ttcactaaac
     5701 ttatcctgca gatacctttc aacttctgtt gcgtctgaac tcacgagtcc gcattgggtg
     5761 tttttaatta cttcagacag gttgtcattg ttgtcgggga tacatattac cggacgatta
     5821 gctcccaaag cttcgaaaaa cttggtagtc attataccga aatagttttt agtattctga
     5881 gaattgcaca gtaccaacaa cagggaacta ttatttaatt ctgcaggcag attttccgga
     5941 gcggtaaagt tatgaaattc tatgaactcg gacaaatgat aactctcagc aatattctta
     6001 acaacaacta tcgactgctc atcaacaaac catttaacaa tagtatttcc tggtgagata
     6061 atcttttttt tgcgcaattc atctaaagct acgaaaagca gcctcgggtt acgaattttt
     6121 tcgttataaa tcctcccaaa ataagaaatg gtgaaaagag aagtggttct tatctcagga
     6181 atgaataaat cttcatcaaa gccattgtaa atcaggtgtg tgtttgggtt atatatagat
     6241 aatgttttga catgccaggg tgatacggtt gtaacaccgg ccgcttttct caatacggtg
     6301 ttacgccgtt tgatatttat tctcttataa attttgataa gtatatttcc aaaaaaaaat
     6361 ggcggacgat gagctatgaa atgattatcg tccggcgact gttctgcaat gtcccgtaga
     6421 tcaacaaata gcggaacatc gagtttacgc gccatacgcg cagctgtagt cagtggaaaa
     6481 gtgaaacttg aactgcagaa tattaaatca tatttttttt cggtaacgaa agattgagat
     6541 ttccggtaaa aataccttcc tttaaaatca atagataaat ccattataaa tttaatcatc
     6601 cattccaacc gtcccaaaat tcctttttca ttcttgtaat aatcaatcgt aagccacgat
     6661 accttctcag gaacatcgta actgtattct gatttttcgt tcacaaaatc cacctgccag
     6721 cctttcctac tgaaatagga aacgaaatac ctcactcgag gtaagtaggc gggcgacaga
     6781 aatgcttctc cgaaaataag taaacgcata atagaattat tttattttag atagttaatc
     6841 agccatcggt acaaatctcg gtgatattgt ccattgtttt tctctaccga tgtattatgc
     6901 caaagtagca ccaattcgcc gttgtgctta ctgactaagt caatcattgt ttttgtaaat
     6961 aagaaagcct catcggtttt taaattcatg tatctctcgt cactcagcgt attgtccatg
     7021 atggtgagcg gatgcagggt gagtgaagtt aattgcaatt ttaccgggtc aatccatttt
     7081 acagctctac aggtgcccag ccggaaaccc gtaatgtctg cgtaagccat agtaaaatca
     7141 tctgtcagtc cggcttcaat cagcgtctgc atatcttccg gttcacggct cattaaaaag
     7201 tgattgcgtg tatatatcgt attttgactg atagtctcat cgagaacttt tttttcattt
     7261 tttatgtact ccggtttcat tccggccaga taagagggat gaagtccgat tttcacgttg
     7321 tgttgttcgc atagtttgaa taatgtgttg aaatccctgt ctctgacatc atgccgcggt
     7381 ttgtcagtca tcagttcacc accgccggtt ttgataaaaa ctatattttc gattttctct
     7441 tccggttttt tgtttttcag tttatctgcc agatcgaata accagggaaa ggtgaaccag
     7501 gggtcattta ttattcctcc cgcaaaactt tcaactgctt ttagtgattc agccggattt
     7561 ttgaaaaagc gtaaaaccgc tccgccgatt cctctgatgt tccgatagtg agccaattgg
     7621 tccacatcat gtgtcaggta gattttactg aatttctctt cgggctcgct gatatcaagt
     7681 cctgtttcgc gtagcaattt tctcaacagt cttccgtatt cgtcaacgac aggtcgttga
     7741 aggaacccag ccctgaaagg caatgatttt atacccggga aacgaccatg actgtctcgg
     7801 atatttctgt tgatgatttc ctcataccgg ctcataagaa aaaaactgct tgctattatg
     7861 tcggcatgga taatgactgt ctcgtctgat ttttctgtta ccggttcacc aaaaagcagg
     7921 ggtatccctt cccacacggt tagtggcagt tgcggaaggg atttttcagt gttataaaat
     7981 tccggattaa aaaaaccgga aggaattatg acaatcttat atttactgaa ttgttcttta
     8041 tcggaagtat aaccaatcag tctgctgaaa gtttcattgg aatcgctgcc cagcaaaaaa
     8101 cgtatgatat agtctgttat ttctgataaa cgttcagcag tcatacaggt ttatttgatt
     8161 ttcattttca tataatgaga gtaggtgagt atgacggcta ttgccaggcc cagccagaga
     8221 tctgtgctgg tgaaaactcc gaggaatgca tccccttcgg gcatcttttt tgcaactaac
     8281 ttcaatatcc aggttagtaa ggcataaatg caaaaaacac cggcaccata taaaaataac
     8341 tttaattttt gattcatgac aaaacaatga ttataagaaa caatgaatat aagtggttta
     8401 tgcaaagtta accatttaat tcattattca gaacttttta cctgaaataa accgtttcgg
     8461 acagatgttc gttcagaaac ctttattaaa attcaagagc aagaaatttt ccggtaacac
     8521 tatcgggatt cccggctact tcacggggac ttccggagca aatgaccctg ccaccaccat
     8581 tgccaccttc gggcccgatg tcaatgatgt agtcgacagt tttgataaca tccatgttat
     8641 gctcaatgat tataactgta ttcccgcgat taaccagtcg gttgagtact cccagaagta
     8701 cccggatgtc ctcaaagtgc agtccggttg tcggctcatc cagcacatat aatgtttttc
     8761 cggtatcccg tttcgatagt tcagttgcca gtttcacacg ctgactttcg cctcctgaaa
     8821 gtgtggtact gctttgtccc agtttgatat atcccagccc tacatcctgt agtgttttaa
     8881 ttttattgag aatataagac tgattttcga aaaattccac agcctggttg atagtcatgt
     8941 taagcacatc tgcaattgac ttacctttaa aacgaacttc gagtgtttcg tgattgtaac
     9001 gctttccgtt gcagttttca cagggtacat acacatcggg gaggaagttc atttcaatgg
     9061 ttttgtaacc attacctccg caggtttcac aacgtcctcc agctgtattg aaagagaagc
     9121 gtcccggttt aaatccccgt atttttgctt cgggcaaggt agcaaacagg ttgcgtatat
     9181 cgctgaatac tccggtgtag gtggcaggat tggatcgggg cgtacgtcca atgggtgact
     9241 gatcaacctg aactatttta tcgatatttt caattccttc gatactgtcg tagggcagag
     9301 gttcctgtag tgaacgataa aagtgctgac tgataatggg ctgtaatgtg ttgttaatca
     9361 gcgtagattt tccactgccc gatactccgg tgacacagat catcttacct aacgggaatg
     9421 tgacatcaag gtttttcagg ttattgcctc tggcacctct aagtgtcaga aattttccat
     9481 tgccttcacg gattttttcc ggaatttcaa ctttcttttt tcctgtcagg tattcagcgg
     9541 taagagtgtc ggcctgaagc atttgtgcag gagtgcctgc aaaaaccagc tcaccgccaa
     9601 gtctgccagc atgcgggccc aaatccaata cataatcagc tgaaagcatc atatccttat
     9661 cgtgctcaac aacaataacc gaattcccgg tgtcacgaag ttgtttcagc gagttgatca
     9721 accggaggtt gtcgcgctga tggagaccta tactgggttc gtcgaggatg tagagtacgt
     9781 taaccaactg agaaccaatc tgagttgcga gccggatacg ctgactttca ccgcccgaaa
     9841 gtgactgaga acttctgtta agcgccagat aattgagccc cacatccagc agaaattgca
     9901 gacgtgacct caactcttta agtatttcgg tggctatgac acgttgcttg tcattaagga
     9961 agggatcaac cttgttcagc cagtccgaaa gttcagcaat gtccatttct gccagttcgg
    10021 caatgttttt gtcgtgaatg cgaaaatata atgattcctt tcgtagtctt tggccgttac
    10081 attcgttaca tttaatggtt cttgagtact gattagccca tttttgttcg gtagcagaag
    10141 ccgagttttc ttgttgcatt tcgatgtact tgataatacc atcataactc aggaaatagt
    10201 tggagttgcc cagtgactca ttttttatat gaagttgctc gtcggtaccg ttcattattt
    10261 catcgatggc atcatcaggc acatcggcga gcggagtttt tagtgtacag tcatatttag
    10321 ccagaatagc ttcaatctgc cagaaaatgg tactgttttt atgtttcccg agcggggcaa
    10381 taccaccgtt gaaaatgctg acggatgtgt ccggaatgat tttagccata tcaattttat
    10441 tgataaagcc cagccctttg catttcggac aataaccgtg aggcgaattg aacgaaaaat
    10501 tatgaggagc cggatcatca tatgagatac ctgaagtagg gcacatcagt ttgcgactga
    10561 aatacctgac ttcgtcttta tctttttcga gcagcataat gataccattg ccgtgttgca
    10621 tggctttctg catcgaatct ttcaggcgtt tgcggtcttt gtcggttacg agcagccggt
    10681 cgattacaat ttcaatatca tgaattttgt aacgatccac tttcatattg tgggttattt
    10741 cgcggacttc gccatccact ctgacatgca ggtaaccttt cttacgaatt tgttcgaaaa
    10801 gatcccggta atgtccttta cgtccgcgca ccactggtgc cagcagcagg gttttctttc
    10861 cttcgtaatt tttcagaaca agatcgataa tctgatcgtc ggtatatttg atcatcgctt
    10921 cacccgtaat atatgaataa gctatacctg tacgggcaaa aagcaaacgc agaaagtcgt
    10981 aaatttcggt ggttgtacca acagtagagc gcggattttt attggtagtt ttctgttcga
    11041 tggaaataac aggactaagt ccggtgattt tatccacatc ggggcgttct atatttccca
    11101 gaaaactgcg tgcataagcc gaaaatgttt caatataacg gcgctgccct tctgcaaaaa
    11161 tagtatcaaa agctagtgaa gatttaccgc taccgctcaa tccggtaatt accgtgagcg
    11221 catcgcgcgg aatggtaaca tcaatatttt ttagattgtg tactcttgcg cctgtaacat
    11281 caacattttc agtagtgtcc ggtaaatgaa tatttttagt tgtatccaaa atttgtcaat
    11341 tttaataact tgcaaagata cagaatttta atagaaaaaa tacgacaccg ttgagccgaa
    11401 gaattaaaaa caaaaatgat ttcagatttg ttttgtgtta atcaaattta ataggttaaa
    11461 tttaacaatt tatattttat atctttgtaa ataaacaatg gttcatgtaa tttctctaaa
    11521 ttacaaagca tccggcgaaa ttttaactat ctgaataact gatatatatc aatctaaaaa
    11581 cataaaaata tgacacaaat taaagaacgt cttgccgcat tgcgtcagtc gatgaaaagt
    11641 gcgggtattt cagcttgcat catacccgga accgatcctc atgcaggtga atatattgca
    11701 gattactgga aagaacgtgt ttggattaca ggctttacgg gatctgcagg tactgtagca
    11761 attactttcg ataaagccgg actgtggacc gattcccgct attttttgca aggagccaat
    11821 caactgaagg acactactat tgacctgatg aaacagggat tacctgaaac tcctgaaata
    11881 attccctggt tgtcggaaga actgaaaagc ggagataaag ttgccgtgaa tgcacagatg
    11941 ttttcggtga atgcttatgc caacatgaaa gctgaactga aaatgagcgg aattgaactc
    12001 gtttcgcttg atttgctgaa agaaatctgg accgatcgtc cggcactgcc gaataatcct
    12061 ttctttgttt ttgatgtgaa atacagcggt aaaaccgtat ccgaaaaact ggttcagctg
    12121 cgtgcagaat tacataaagc acatgcacag gtatttgtga tgtcagctct cgacgatatt
    12181 gcctggttat tcaatattcg cggaaacgat gtagctttca atccggtagt gattgcgtat
    12241 gcactcgttg atgaggaaaa agcaatgctt tttgttgata tggctaaatg cacggatgct
    12301 tcgtgcgatt atctaaacgg caacggtgtt gtcattgcca actatcagga tatttacacc
    12361 caattacgtc aaattccggc agataaagtt gtgatgtatg atggaaacaa actcaatcag
    12421 tcgttgtttg aggcattacc cgctgaatgt gaaaagaggg taggaatgtc gccggttttc
    12481 aagctgaaaa gtctgaaaaa tgaagtcgaa atcgccggaa taagaaaagc catgatcaaa
    12541 gacggagtag cgttaaccaa gttttttatc tggctcgaag agcatgtgaa agatggaagt
    12601 ttgacagaag tgtcgattgc agataaattg cgcggattca gggctgaaca atcaaacttt
    12661 ataggtgaaa gttttgatac gattgccggt tatgcaggcc atggagctat tgttcattat
    12721 tcggccacac ccgaaacggc atcgacgtta aaacctgaag gaatacttct gctcgactcc
    12781 ggagggcaat acctcgaagg aactaccgac ataacacgta caatcacatt gggtaaaccc
    12841 acttcgaagc aaaagaaaga ttatacgctg gtattaaaag gtcatattga tctggcaatg
    12901 gcgaaatttc cggtgaa
//
LOCUS       DAOPYB010000075        12637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5541_length_12637_cov_8.782388, whole genome shotgun sequence.
ACCESSION   DAOPYB010000075 DAOPYB010000000
VERSION     DAOPYB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12637
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5541_length_12637_cov_8.782388"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PKH58_07240"
     CDS             <1..459
                     /locus_tag="PKH58_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88860.1"
                     /translation="QESQNKIAGQEVIYYHLKVKNHKPRTTVSKCRVILKEIHSKDPD
                     TKKYTKLTLTVPPRFQWAPAETSPPAVDFVDEQILDFGYLEKGADEFRPSLSPSFNNF
                     KGNLKANGIIKYTLEVQANNAKTVRVELEVSWDGNWTSDLEKMKLIIEKK"
     gene            504..947
                     /locus_tag="PKH58_07245"
     CDS             504..947
                     /locus_tag="PKH58_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88861.1"
                     /translation="MRSKVITIFLASSFYISFAQTNENIIGLFGECKEGYFACSQIEF
                     KSDSTFEYDVFFDVGGWQCWTGRWSIDSNIITINTFSQPMTEEENSLVNQSYSFVSTD
                     FITDLKFRIKKNRLYIYDFEKNKISNRYYFRKTSIDKKEYKNCNK"
     gene            1247..2665
                     /locus_tag="PKH58_07250"
     CDS             1247..2665
                     /locus_tag="PKH58_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX88862.1"
                     /translation="MKKNPFRLFLSVLLIAPCFAFTQSVSSESAASKALSFINTATYN
                     ASDLIIQPLSSGGIQSAWLVRLPDKRFVIISADSLMQPVLAYGDSISGMESDKFSTWQ
                     KIMASDYTNRLVAIRSGYIPSQKALWSEPKSRLFQQWPPAGTTPTGGWLSTNWTQSSP
                     YNKLCPMDLNAGNRSVVGCPATAMAQICNYYMTVNSTQFSDADDYYHNFGNGNRYWID
                     DAHAERDFPDFPELNQWLDTIQQLYLQNLPITDSMKAALSFAAGVAAKQVYSASVSGT
                     YGIDQAQMAYQRFNFDLSELIGPASFELTSRVAADMKAAMVDHLGIVDAAVTVGHNVV
                     IDGYNTDEFYHFNFGWGGAYNGWYTLPPSGMPYSLNVIEGVVVNIKNTYAHASPWIKN
                     TPEPAFAFMPGDNLRISGIDITGSCELRLFNVSGALVLVREFTAVKGNEVMLNTPGLS
                     PGIYLCLVHSAGKMCTGKAVKP"
     gene            complement(2802..3566)
                     /locus_tag="PKH58_07255"
     CDS             complement(2802..3566)
                     /locus_tag="PKH58_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88863.1"
                     /translation="MKRIIAKLNHYNLILRIFENHPEVFEKKPGWKDILAEFEERVQR
                     ITAILSDVNRPVQYFYDTRRDAENKLGEKVVNFAGMGISFAVSLNDNAMMQRMKTYRG
                     QVRKVSGFRLYNSAKFVAEDLYKVDTSWRGNTMSDQEIDKFVKQAEDFRETLDWLHHQ
                     LAMRRALRKELAGLIAQNNIWLHDILDGIYRNAEDDCPDFCNEYRTLRFKKRKRRRRN
                     SETAGNEAAAEAMVTPDGKVINMPKERESGRRRVKN"
     gene            complement(5222..5677)
                     /locus_tag="PKH58_07260"
     CDS             complement(5222..5677)
                     /locus_tag="PKH58_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88864.1"
                     /translation="MWLMEWLPATYSLQNITARKQFAIIPGHSHLATGGSAVSGRSFA
                     GYASSYATFSGEIATLSASTREMSTDFYRSAAVLHAVSKATGISGKCFRIFSRRLHHF
                     TGGAGLLSMVFHHFSNGIQAESVLFHPFSGKFHQMSAPFRLMREKFHRL"
     gene            5962..6291
                     /locus_tag="PKH58_07265"
     CDS             5962..6291
                     /locus_tag="PKH58_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX88865.1"
                     /translation="MSKIEVKTFGETLRKLREDNSMPLRKLAALLDLDQSTLSKIERN
                     ERKANSELIEKLSSIFDVDKKTLQISFMSDKVAYELLEEENPKDILKVVEQKISYLKL
                     SKKPTNV"
     gene            6284..7165
                     /locus_tag="PKH58_07270"
     CDS             6284..7165
                     /locus_tag="PKH58_07270"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HNX88866.1"
                     /translation="MFNESIIIKSRVAELYLARSKKLFPEFESQKPFLKWAGGKTQMI
                     SDILKFAPKNFNKYIEPFIGGGAVYFNLNHPKSIIADSNEELIITYTQVKNNIEEVIE
                     ILENYINSEDFFYKIRALDINKLSHSERAARLIYLNKTCFNGLFRVNKKGEFNVPFGK
                     RSGPFLNKGNLIGASLYLQDTDIYHSDYKDVLQKYAQKGDFVFLDPPYQPVGKFSDFK
                     RYTKEFFYENDQIELAQIFKDLTNKGCYVILTNSHHPMILELYKDFHIEIIETKRMIS
                     SDPKTRTGVDIIVLGGI"
     gene            7162..8211
                     /locus_tag="PKH58_07275"
     CDS             7162..8211
                     /locus_tag="PKH58_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNX88867.1"
                     /translation="MSIQLEIDSFTDQFQYFPGTRYMGSKNKIILDIWHHLGKLEFES
                     VIDAFAGSNVVSYFLKSKGKRVITNDFMTFSYITSKAIIENSSELLDKEDIKFLLKEN
                     INKGFIQRTFRDIFFSEIENIFLDRVRQNISELNNEYKIALALSSLVRACIKKRSRGV
                     FTFVGERYDDGRSDMKKSIEEHFLESINIFNKAVFNNGFQNQSYNIRSEQFNAKADLV
                     YLDPPYYTPKSDNDYVRRYHFVEGLVRNWEGIVIQEHSVVKKFESYKSPFAKKDTAYS
                     AFDELIKKNKESMIAISYSSNSLPTKDELIEMLKKYKSDVVVHEIDHTYSFGNQNHKI
                     GNKNNRVKEYLFIGI"
     gene            8215..9246
                     /locus_tag="PKH58_07280"
     CDS             8215..9246
                     /locus_tag="PKH58_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88868.1"
                     /translation="MRIQSNDLPQADRIESVVAATHAIANGARTDIEIADQVPGIEGD
                     DRQGRYYRRAAEILGFITNNRNNAAITTKGQLVANNPSLYNPIVIASVLNLNLYQKLL
                     PYIELHPEGVTRNQIIDYLQLISVANIGPSMIPRRISTILGWIRTLGVIEQVGERYRI
                     ANAIIPSIPLITLNDIDQPILPATGGLHEFEAVEQRISSAREIITIYKNQARLDRANN
                     THMQLVNIVAERIRVAGGIPKSNQFIDLASTLDQDYIFEMKSTTVENVKSQVRKGISQ
                     LYEYRYLENKPNANLVLVLESPLIGDNSWMIDYMENDRNIHLVWDGDNNLYGTGRTRE
                     QLGFLGLLP"
     gene            9474..10001
                     /locus_tag="PKH58_07285"
     CDS             9474..10001
                     /locus_tag="PKH58_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX88869.1"
                     /translation="MSRKYKFHNPDGVYFITFAVQGWADVFTRNEYKDILIENLAWCQ
                     KNKGLEIFAWCIMTNHVHLIARAEEGFSLSDILRDFKKFTSKAVLKAIRENNRESRKE
                     WLLKQFETPDGNRFWRSDNQPIELWSNAVIDQKLNYIHQNPVVEGLVFRAEDYVYSSA
                     SDYAGNKGMLDIFVL"
     gene            10131..10712
                     /locus_tag="PKH58_07290"
     CDS             10131..10712
                     /locus_tag="PKH58_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88870.1"
                     /translation="MNRLRLTISILTLIVLTISALGQENSIDHFKVKKNKIDVYFYDP
                     FEALSKSENFSSKSDKEKSDALDNYLHNHALYLFVLNDAKTKDITYFCIRGNPEKVNT
                     KMFYKVEVIQGVTNSPFNPNTDSYSDKVVKVIDKVNCGGTLFENSAIFSKPENKDKVG
                     NGIQLYGYFRRVQPFSEIRTSMTSIIEQYLQEK"
     gene            10876..11343
                     /locus_tag="PKH58_07295"
     CDS             10876..11343
                     /locus_tag="PKH58_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88871.1"
                     /translation="MKRHLICFTLTVLTVILFSCNTSNNKAGNNPSAAVNTHSMALTE
                     MQTAKLDSIVGLIIDISANDFYKNQRPEPVDFKNVKLKYIKKTNGEELYILCGRFVTS
                     DKQEIQFATVKNIDYEQWIGNSALTYCQDSKEIAYTKEDLSAALKNRFDSLKK"
     gene            11387..11932
                     /locus_tag="PKH58_07300"
     CDS             11387..11932
                     /locus_tag="PKH58_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88872.1"
                     /translation="MRIFDKIFRSGSKAKVLTVEEVEKRSIELKVKHEKAKQAVKTAL
                     ENYNEISCQCAYPRFQQLIGIDCAKTRDSFKCLDTDLLISISKEYFDISDSTLTDENT
                     NAKWICKKCGSTYEYGWSDFSIYVERQKLELVDLKVELTGKATRKPIPLYLGLIGHSH
                     PSKAEMTSVEFDDFEKYILEK"
     gene            12070..>12637
                     /locus_tag="PKH58_07305"
     CDS             12070..>12637
                     /locus_tag="PKH58_07305"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNX88873.1"
                     /translation="MRIELRYFTGTGNSYRIIDTCRTVFNQSGHTVTVSEINAEEKEI
                     AESGLLGFCFPVYAFGIPRICRKYLASVRSFKNRQKAFVLITAGDSDESGFATAECEK
                     ILKRKNCDIVYTGVIQMPINWKTSPEPPYPPAKDEALEIIKAGVGQAQVIAGEILNGV
                     RKHHQFSYPARYTKWRFYKDYWLFKYMGL"
BASE COUNT         4059 a         2399 c         2623 g         3556 t
ORIGIN      
        1 caagagtctc aaaataaaat tgcgggtcaa gaagtaatat attatcatct gaaagtaaaa
       61 aatcataaac ctagaacaac tgttagtaaa tgtagggtca ttctaaagga aattcattca
      121 aaagatccag atactaaaaa atatacaaaa ttgactttga cagttccacc tcgttttcaa
      181 tgggctcctg ctgaaacgtc gccacctgca gttgattttg ttgatgaaca gattttggat
      241 tttgggtatt tagagaaggg agctgacgaa ttcagaccat ccttatcacc tagctttaat
      301 aacttcaagg gaaacttgaa agcaaatgga ataatcaaat atacacttga agttcaagct
      361 aataatgcta aaacagtccg agtggagctt gaagtatcat gggatgggaa ttggacttct
      421 gatcttgaaa aaatgaaact aataatagag aaaaagtagc aacaatcgca agtcacggaa
      481 ctattatggt caatttatca agaatgagat caaaagtaat tacaattttt ctggcatcaa
      541 gtttttacat ttcttttgcc caaacaaatg aaaatattat tggattattc ggggaatgta
      601 aagaaggata ttttgcttgt tcacaaattg agtttaaatc tgattcgaca tttgaatatg
      661 atgtattctt tgatgttgga ggctggcaat gttggacagg taggtggtca atagatagca
      721 atataataac aataaataca ttttctcaac caatgactga agaagaaaat tcattagtaa
      781 atcagtcata tagcttcgtg agcactgatt ttataacaga ccttaaattt cgaattaaga
      841 agaacagact atatatctac gactttgaaa aaaataaaat aagtaaccgc tattacttca
      901 ggaaaacctc aatagacaaa aaagaatata aaaattgcaa taaataaaac tggtataacc
      961 cactgctggc gtggctctga tgactatcgg aatgccatcc gtgccaatca attttacccc
     1021 gttggtgcac aactttcttt catatgcgtg cccgaggtct tactttcaaa tatttccatc
     1081 gagttacagg taagttgtca tcaggagaac tgaccgaaat tcattctgaa attttttctg
     1141 ctccaattca tatttaaata atctcaaagt gatgttgcct gtttcagccg gttttgtact
     1201 tttgatcccg atagctatcg ggactgattt ttaacatatt ttgcaagtga aaaaaaatcc
     1261 cttccggctg ttcctttcag tcctgcttat cgctccctgc ttcgccttta cacaatccgt
     1321 cagcagcgaa tccgccgcat ccaaagcgct gagtttcatt aataccgcca catacaatgc
     1381 aagtgatctc attatacaac ctctcagctc cggcgggata cagtcagcct ggctcgtcag
     1441 gctgccggat aagcgctttg tgatcataag cgccgattca ctcatgcagc ctgtccttgc
     1501 ctatggcgac agtatttccg gtatggaatc cgacaaattt tcaacctggc agaaaataat
     1561 ggcttcggac tataccaaca ggcttgttgc tatccggagc ggttacatcc cttcacaaaa
     1621 agcgctatgg tccgaaccca aaagcaggct tttccagcag tggccgcctg ccggaacaac
     1681 acccaccggc ggctggctca gcacaaactg gacacaatcg tcgccataca acaagctttg
     1741 ccccatggac cttaacgccg gaaaccgctc agtagtagga tgccccgcca cggccatggc
     1801 tcagatatgc aactattaca tgacggtaaa cagcacgcaa ttcagcgatg ccgatgacta
     1861 ctaccacaat ttcgggaacg gcaacaggta ctggattgac gatgcccatg ccgaacgcga
     1921 tttccctgat ttccctgagc tgaaccaatg gctcgatacc atacagcagc tatacctgca
     1981 aaacctcccg atcaccgaca gcatgaaggc ggcgctgagc tttgcagccg gtgtggcggc
     2041 aaaacaggta tattcggcat ctgtgtcagg cacatatgga attgaccagg cgcagatggc
     2101 ctaccagcgc ttcaattttg acctttcgga actgatcggc cctgcaagct tcgaacttac
     2161 gagccgtgtt gccgccgaca tgaaagcagc catggttgac cacctgggaa tagtggatgc
     2221 cgcggtgacg gtagggcata atgtagtgat tgacggctac aataccgatg aattttatca
     2281 tttcaacttc ggctggggtg gagcctataa cggatggtac accctgcctc cctcaggaat
     2341 gccttacagc ctcaacgtga tagaaggggt agtggtaaat atcaaaaata cctatgcaca
     2401 tgcaagccca tggataaaaa acacaccgga accggctttc gcttttatgc ccggtgacaa
     2461 tttgcgtatc tcaggcattg acatcaccgg cagctgcgaa ctcaggttat tcaatgtttc
     2521 cggcgcactg gtccttgtca gggaatttac cgccgtcaaa ggcaatgaag tgatgctgaa
     2581 tactcccggt ttaagtcccg gcatttacct gtgcctggtt catagcgctg gcaagatgtg
     2641 caccgggaaa gccgtaaaac cctgaaaaag ttcattggat tcctggtaaa ttgtcaattc
     2701 tcaattttca attgtccacc tgccccttac gcgctatcct tcccgtggca ggcaggtttt
     2761 caattctcaa ttttcaattt tcaattttca attgtccatt gtcaattctt aaccctccgt
     2821 cttccgctct ccctttcctt cggcatatta attaccttcc cgtccggcgt caccattgct
     2881 tcggctgcag cttcatttcc ggcagtctct gaattcctgc gcctgcgttt gcgttttttg
     2941 aagcgcagcg tccggtactc gttgcagaaa tcgggacaat cgtcttccgc attgcggtaa
     3001 ataccatcca gtatatcatg cagccagatg ttgttctggg cgatgagtcc ggccagttct
     3061 ttcctcagtg ccctgcgcat ggcaagctgg tgatgcagcc agtccagtgt ctcgcggaaa
     3121 tcttcggcct gttttacaaa cttgtcgatc tcctggtccg acatggtgtt accacgccac
     3181 gaggtatcca ccttgtacag gtcctcggcc acaaatttgg cgctgttata aagcctgaac
     3241 cctgatacct tgcgtacctg ccctcggtag gtcttcatgc gctgcatcat cgcattgtcg
     3301 ttgagcgaca cggcaaagga gatgcccata ccggcaaaat tcaccacctt ttcacccagc
     3361 ttattttcag catcgcggcg ggtatcataa aaatactgca ccggccggtt tacatccgac
     3421 agtatggcgg tgatgcgctg cacacgctct tcaaattcag ctaaaatatc cttccatcct
     3481 ggctttttct caaaaacttc aggatggttc tcaaaaatgc ggagaataag gttgtaatgg
     3541 ttcagtttgg ctatgatacg tttcacgtga atataattta atcgttagcg gcccgcctgc
     3601 cttagttata ccgggataag cggcgcagga gtaaacaacg ttttaagtat taaaggtttc
     3661 gtgcccgata tgtcctgagt attaaatatc cggttctgaa tcttcatgta atgctaatat
     3721 ctgctttcaa tatcgcctgc agcctgaaag gcttccaacc actatcgtga tcaattttca
     3781 atttcccatt tcccattttc aattctcaat tttcaatttt caattttcaa ttttcaattt
     3841 tcaattgaat agcgcttaac aaaaataaaa aatccccata attccctgca aaagagttat
     3901 aaacatacac aggattactt tatttccctt gtgtttgcct ttgcctgccc ggcaaaacag
     3961 cggatgaatg aacgaatttc gttgcacaat gccgtgaact ccttccggca ggagcatcca
     4021 ccggaccggc tgtcagtgat tatctgccgg cagttgtaaa acataccgga tgctttgcag
     4081 caaagccagt ggagggatta aacgcctcag tccatcaata acaaaacgaa tactcccggt
     4141 ggtgccggca attgatcttt gcatttcctt cggataaagg ataaaacagt catgacaaag
     4201 gatcaaaatc ttatgagaag tgtccggcag ggcaggaggg aggataaaaa ccggttatgc
     4261 aaggttgaat atctttggag cgaggagcgg aatgcgatga ggaaacctca gcagcgcagg
     4321 agaaaacccc ggcaatgttg ccggagactg cggaaagatt tacataacaa ttaaacagtt
     4381 atgagaagac accggaatct tccggcatga gctgtgcagt gttagcggaa agtcctgcag
     4441 tattgtataa ggtgttgaaa cttttaccgg aagttgcaac acagtgagag tgaggataaa
     4501 atcccccgga gtgagcgtgt gttatattga agtgaccatg tgtcgcatta tacatcagtc
     4561 gaaatggtta tgacccgggt gcagcatgat tgaagtgagc atgtgacggg tagaagtgaa
     4621 cctgtgttct gataaagtaa gcttctgtcg tgtttgactc cggttgaaaa agttatgaca
     4681 agagagcgaa aacaaagcag tgaatgtatg ttgatataaa gtaaacatgt gaccggttaa
     4741 agtgagcctg tgtcgaaata acgcacttgt ataaatcttc tgacgtacat gcgataacgc
     4801 tgaagtgact acttggcaat ataaagttag tatatggcag gttaaagtaa ccgtgtgacg
     4861 ggattgacga ccgatgaaaa tttattgaca agagagcggc aactgagaag tgaacgtgtg
     4921 gcggccagaa gtaattatct gtcctaataa agtgacctta tgttgcttta tacaactgtt
     4981 gaaaatttac tgacaacagc gcgacaaacg ggaagtgaac gcatgacagc cagaagtgag
     5041 tgtgtgtcct gttaaagtga ccttttgtta tgtgttacaa acgatgaata tcttatggct
     5101 aaagcgtgac atctcagaag ttagttaatg gcagcctgaa gtgacctcat gtcctgttaa
     5161 agtaaacggc agcgcttgct gattctcgat gaaaacttcc ggataagact gcaacgtgca
     5221 ttcacaaacg atgaaacttt tcccgcataa gccggaaagg cgctgacatc tgatgaaatt
     5281 tcccggagaa aggatgaaaa agcactgact ccgcttgtat cccgtttgag aaatgatgaa
     5341 acaccatgga caagagcccg gcgcccccgg tgaaatgatg taaacgccgt gagaagatac
     5401 gaaaacattt accggaaatt ccggttgcct tggacacggc atgcaaaacc gccgcggaac
     5461 gataaaaatc cgttgacatt tcccgcgtac tcgctgacaa ggttgcaatt tctcctgaga
     5521 aagtggcgta gctgcttgca taaccggcaa aactcctgcc gctgactgct gatcctccgg
     5581 tggcaaggtg cgaatgcccg gggatgattg caaactgttt acgagctgtt atattttgca
     5641 gggaataagt tgcaggtaac cattccatta accacattgg gtttcgcagc agggaaaaag
     5701 cattgaacta aagtccaaac cattgccgat ggcatgcatt atacagaata tgataataaa
     5761 ttcctgcccg gcaaagcaat aatagctgat ttgttaatat attagtagga taatatatct
     5821 tttaatttat agagaattgg acattttatt tcatttcgta ggctgcttat atcatcaacc
     5881 ctgttaataa ctttctagga ataattgttc aggttataga attgcccatt atttttggac
     5941 ataatttgtc aagaattatt tatgtctaaa atagaagtta agacttttgg tgaaactcta
     6001 cggaaattgc gtgaggacaa ctcaatgcca ttgagaaaac ttgctgcttt acttgatctt
     6061 gaccaatcaa cattgagtaa aattgagagg aatgaacgca aagcaaattc ggaactcatc
     6121 gagaaacttt caagcatttt tgatgttgac aaaaagactt tacaaattag tttcatgagc
     6181 gacaaagtcg catatgaact tcttgaagaa gaaaatccca aagacattct aaaagtagta
     6241 gaacagaaaa taagctatct taaattaagt aaaaagccca ctaatgttta atgaatcaat
     6301 aatcataaaa tcgcgggtag ctgagttata tctcgccaga tcaaagaaat tatttcctga
     6361 atttgaaagt cagaaacctt ttttaaagtg ggctggcgga aaaacacaga tgatttctga
     6421 cattttgaag tttgcaccaa agaattttaa caagtatatt gaacctttta ttggtggtgg
     6481 tgctgtctat tttaatctca atcatccaaa atcaattatt gccgactcaa atgaagaatt
     6541 gataataaca tatacacagg taaagaataa tatcgaagaa gtgatagaaa ttcttgaaaa
     6601 ttacattaat agtgaagatt tcttttataa aatcagagct ttagatatta ataaattaag
     6661 ccattctgag agagcggcaa ggcttatcta cctaaacaag acttgtttta acggtctttt
     6721 cagggtgaat aaaaaaggtg aatttaatgt accttttggc aaaaggtctg gaccatttct
     6781 taataaggga aacttaattg gagcgtctct ctatttgcaa gacacagata tttaccattc
     6841 tgattataag gatgttttgc agaaatatgc tcaaaaagga gattttgttt ttctcgatcc
     6901 accataccaa ccagttggga aattttctga tttcaaacgt tatacaaagg agttttttta
     6961 tgaaaatgat caaattgaat tagctcaaat ttttaaagat ctaactaaca aaggctgtta
     7021 tgtaatactg acaaattcac atcatccgat gattctggaa ctctataaag acttccatat
     7081 agaaattatt gaaactaaac gtatgataag tagtgatccc aaaacaagaa caggagtcga
     7141 cattattgtt ctaggaggca tatgagtatt caattagaga tagatagttt taccgaccaa
     7201 tttcaatatt tcccaggtac aagatatatg ggaagtaaaa acaaaatcat acttgatata
     7261 tggcatcatt tggggaagtt ggagtttgaa agtgttatag atgcttttgc tggtagcaat
     7321 gtagtaagct attttctgaa atccaaaggc aaaagagtta taaccaatga ctttatgact
     7381 ttcagttata ttacatcaaa ggcaataata gagaactctt ctgagctgct tgataaagaa
     7441 gatattaagt ttttattgaa ggaaaatatt aacaaaggtt ttattcaaag gactttcaga
     7501 gatattttct tttcggaaat tgaaaatatt tttcttgata gagtaagaca aaatatttca
     7561 gaattaaata atgaatataa aattgcattg gcactttctt ctttagtacg tgcttgtata
     7621 aaaaaacgat caagaggagt ttttactttt gttggagaac gttatgatga tggacgttca
     7681 gatatgaaaa aatccataga agaacatttt ctagagagta ttaacatttt caataaagca
     7741 gtttttaata atggttttca gaatcaatca tataatattc gttctgaaca gtttaatgct
     7801 aaagcagatt tagtatattt ggatcctcca tattatacac ctaaatcaga caacgattat
     7861 gtgagaagat atcactttgt tgagggtctt gttaggaatt gggaaggcat tgttattcaa
     7921 gagcattcgg ttgtaaagaa atttgaaagt tacaaatcac catttgcaaa aaaagataca
     7981 gcctactctg cttttgatga acttattaaa aaaaataagg aatctatgat tgcaatttct
     8041 tattcttcta atagcttacc gactaaggat gaattaattg agatgcttaa aaaatataaa
     8101 tcagatgttg ttgttcacga aattgatcac acatattcat ttggtaatca gaatcataaa
     8161 attggaaata aaaataatag agtaaaggag tatttattta taggaatcta gaatatgagg
     8221 attcaaagta atgatttacc tcaggctgac agaattgaat ctgttgtagc agcaactcat
     8281 gcaattgcaa acggtgcaag aactgatatt gaaattgcag atcaagttcc tggtatagaa
     8341 ggcgatgata gacaaggcag atactataga agagcagcag aaattttagg ctttattaca
     8401 aacaatagaa ataatgctgc catcacaaca aaaggtcaat tagttgctaa taatccttct
     8461 ttatacaatc caattgttat tgcatcggtt cttaacctta atttgtatca aaaactatta
     8521 ccctatattg aacttcatcc cgaaggcgtt acccggaatc aaatcattga ctatttacag
     8581 ctaatttctg ttgctaatat tggcccatca atgataccga gaaggatttc tacaattttg
     8641 ggttggataa ggacattagg tgtcattgag caggttggtg aaagatatcg tatagcaaat
     8701 gctattattc caagtattcc acttataaca ttaaatgata ttgatcaacc aattttacct
     8761 gcaactggcg gtttacatga gtttgaagca gttgagcaga gaatatcaag tgctcgtgaa
     8821 attatcacaa tctacaagaa tcaggctaga cttgatcgag caaataacac tcatatgcag
     8881 cttgtaaata tagttgctga acggataagg gttgctggag gcattccaaa atcaaatcaa
     8941 tttattgact tagcatcaac acttgatcaa gactatattt ttgaaatgaa atctacaaca
     9001 gtagaaaatg ttaaatcgca agtaaggaaa ggcataagtc aactttatga atatcgttat
     9061 cttgaaaata aacccaatgc taatttagtc ttggttcttg aaagtccatt gattggagat
     9121 aattcttgga tgatagatta tatggaaaac gataggaata ttcatttggt ttgggatgga
     9181 gacaataatc tttatggtac aggaagaaca agggaacaac ttgggtttct tggccttctt
     9241 ccctaaaaca aatttttatc aaggagatga tgagcacaat acatgaaatg caatagcgcc
     9301 tgccactaat aaataggaac attaacggcg ctcagcgcac ggcgattatc agtttcactg
     9361 agctcgccct ttaagaaact tttatccata tgacccccgc tggcgcggat ttgtaatccg
     9421 tgccaaccga attccagtta accgttaatt ttactttcag aattggattt acaatgagta
     9481 gaaaatacaa atttcataat cctgacggag tatattttat aacctttgct gtacaagggt
     9541 gggcggatgt gtttacccga aacgaatata aagacatact gatcgaaaac ctggcgtggt
     9601 gtcaaaagaa taaaggtctt gagatttttg catggtgtat catgacaaat catgtacacc
     9661 tgattgcccg ggcagaggaa ggcttctcat tatctgatat attaagagat ttcaagaaat
     9721 ttaccagcaa agccgtgctc aaagctatca gagagaataa ccgtgaaagc aggaaagagt
     9781 ggctgttaaa gcaatttgag acgcccgatg gcaacaggtt ttggcgttca gataatcaac
     9841 cgatagaact ctggagtaat gctgttattg atcagaagtt gaattacata catcagaatc
     9901 cagtagtaga aggcctggtg ttcagggctg aagattatgt ttatagcagt gcatcagact
     9961 atgccggtaa taaaggtatg ctagatatat ttgttctctg atgaatgtaa gtatagggag
    10021 gcacggatta caaatccgcg ccaacgaggg ggaggatata acgaatattg aagagatgtg
    10081 cgaaatgggg caggtatctg cttgttatgc attattataa atacaaaact atgaatagac
    10141 tccgattgac aatcagcatt ttgaccttaa ttgttctgac tatatcggct ttaggacaag
    10201 aaaattctat tgaccatttt aaagtaaaaa agaataaaat agacgtgtat ttttatgatc
    10261 catttgaagc tttatcaaaa agcgaaaatt tttcttcaaa atccgataaa gaaaaatctg
    10321 atgcccttga taattatctg cataatcatg ctttgtactt gtttgtgctg aatgacgcta
    10381 agacaaagga cattacatat ttttgtattc ggggtaatcc tgaaaaggtg aatacaaaaa
    10441 tgttttataa agttgaagta attcaagggg ttactaatag tccatttaat ccaaacactg
    10501 atagttattc agacaaggtt gtgaaggtaa tagataaagt gaattgcggc gggactttat
    10561 ttgaaaattc agctatattt tccaaacctg agaataaaga taaagtaggt aatggaattc
    10621 aattatatgg atattttcga agagtgcaac ctttttctga aataagaacc tcaatgacta
    10681 gtataatcga acaatactta caagaaaaat aacaatagat aataattaag gcttcgtatg
    10741 gtcgaggcac gaaaaccaac ttgagacagt gttccacgga atcatgcaac attcgctggc
    10801 aggaatttac cttcgttgaa agttgcaatc aataccagcc agattcaggt taattgaata
    10861 tcaaaacgca aaaaaatgaa gagacacttg atttgtttta cactgacagt tttgactgta
    10921 atcctgtttt catgtaacac cagtaataat aaggcgggta acaatccatc tgcagcagta
    10981 aacactcaca gcatggcttt aacagaaatg caaaccgcca aattggacag cattgttggt
    11041 ttgatcattg atatttctgc caatgatttt tataagaatc agcgacctga acctgttgat
    11101 ttcaaaaacg ttaaacttaa atatattaaa aaaacgaatg gggaagagtt gtatattcta
    11161 tgcggccggt ttgtaacaag cgacaagcag gagatacagt ttgccacggt taagaatatc
    11221 gactacgaac aatggatagg caatagcgct ttgacatact gccaggattc caaagaaatc
    11281 gcttacacta aagaagacct gtcggccgca ttgaaaaaca ggtttgactc tttaaagaaa
    11341 taatgaataa aaacttccgg caagggagta atactgaaaa tatgatatga gaatattcga
    11401 taaaatattt agatccggca gtaaggcgaa agtcttgaca gtagaggaag ttgagaaacg
    11461 gagcattgag ctgaaagtaa agcatgaaaa agcaaaacaa gccgttaaaa ctgcactgga
    11521 gaattacaat gaaatatcct gccagtgtgc ctacccaagg tttcagcagt taattggtat
    11581 tgactgcgca aaaaccaggg attctttcaa gtgccttgat acagaccttc ttatcagtat
    11641 ttcaaaagag tattttgata tttcagacag taccctgact gatgagaata ccaatgcaaa
    11701 atggatttgt aaaaaatgtg gctctaccta tgaatacggg tggtctgact ttagtattta
    11761 cgttgaaagg caaaaattag aactggttga cttgaaagtc gaactaacag gtaaagcaac
    11821 acgaaagcct atacctcttt atttgggtct tataggacat tcacatccat cgaaagcaga
    11881 aatgacctct gttgaattcg atgattttga gaaatatata cttgagaaat gaagtgaatg
    11941 ctctttgcct ggaaggatta tccggctctg tctcgtgaga cctccttagc taagtggcta
    12001 atcggggccg gcgggggaaa tataaaatac tctctgtgaa tcataagact tattacagct
    12061 aaatcataca tgcgaatcga gttaagatat tttaccggca ccgggaattc atacaggatc
    12121 attgatacct gcaggacagt cttcaatcag tcaggccaca cggtaactgt atccgaaatt
    12181 aatgcggaag agaaggagat tgctgagtcc ggcctcctgg ggttttgttt cccggtgtat
    12241 gcatttggca tcccccgcat ttgccgcaag tacctggcat cggtccgcag cttcaaaaac
    12301 cggcaaaagg cattcgtgct cattacagcc ggcgattcgg atgaatcagg ctttgcaacc
    12361 gcggaatgcg aaaagatact gaaacgaaag aactgcgaca tagtgtacac cggggtgata
    12421 cagatgccga taaactggaa aacctcccct gagccgccgt atcccccggc taaagacgaa
    12481 gctttggaga tcattaaggc aggggtgggg caggcgcaag tcattgccgg cgagatactc
    12541 aacggcgtaa ggaaacacca tcagttcagc tatcccgcgc ggtacactaa atggcgtttc
    12601 tacaaggatt actggctgtt taaatatatg gggctgc
//
LOCUS       DAOPYB010000076        12493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5643_length_12493_cov_9.133382, whole genome shotgun sequence.
ACCESSION   DAOPYB010000076 DAOPYB010000000
VERSION     DAOPYB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5643_length_12493_cov_9.133382"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(567..1934)
                     /locus_tag="PKH58_07310"
     CDS             complement(567..1934)
                     /locus_tag="PKH58_07310"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HNX88874.1"
                     /translation="MKILQHKSLLFLLGFTSFFIACNTTTEVSVSDDATIESMYFQNN
                     DSSPNLSDAVFIIDDENGLIYNVDSLPYQTRVDSAYASFSFASTNGYLINDTFPETAY
                     TTSTAYDFSKPVKLTNYASDGKTKKEYTIKVNVHQVETYLHVWKNPVEMITENASDNQ
                     KAVLLNNKYYFFTSNGNSTQLYSSTDASSWLHESNPTGLPANAVFRNMIVCNDQILLL
                     HNGNELYTTRNGVSWEKHLLNGDNNYDFLVLLFNFKNQYWSVAQNKTTKRLNIASSSD
                     GINWTVRNALSARFPVSDFAATTFTPRLGREKIIIVGGKDASGKKLNTRWSAENDPTT
                     DSLHWVNLNNNHSTFEAISDAGICYYGSKLLLIGGTDNYEQLKDTSVQMRQSLDEGLS
                     WAKPDTSLNMLPASFTYRKNASVIHNKTNNTLYIIGGKGMFLPLSDVWTVKANFYNFV
                     DPSKY"
     gene            complement(2078..2851)
                     /locus_tag="PKH58_07315"
     CDS             complement(2078..2851)
                     /locus_tag="PKH58_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNX88875.1"
                     /translation="MKIISYNVNGIRAALSKGLESWLKDTNPDVLCLQETKAQPEQID
                     TSIFNQYGYTCYIHSALKKGYSGVAIFTKIPPDNVVIGMDNHKYDSEGRVLRADFDDL
                     TVVCVYIPSGSSGGERQQFKMEFLNDFSQYISDLRKTRPNLVVCGDYNICHKPIDINH
                     PERQAGVSGFLPEERAWLDEFQLTGMVDSFREFDKSAEKYSWWSFRTDARSRNMGWRI
                     DYHWVSQPVKSSLKSAAILSDAVHSDHCPVMVELETEGA"
     gene            complement(2984..3742)
                     /locus_tag="PKH58_07320"
     CDS             complement(2984..3742)
                     /locus_tag="PKH58_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX88876.1"
                     /translation="MIEVSHLFKSFDGEQVLKDISAVFETGKCNMIIGQSGSGKTVLM
                     KSIIGLHRVDSGKIEYDGRNLPDMRMRDIRRLRREVGMLFQGSALFDSMTVMENVMYP
                     LEMFSSSSRKEMENRTKFCLDRVNLNEHSYHLFPSEISGGMQKRVAIARAIALNPKYL
                     FCDEPNSGLDPKTSLIIDQLIYEITHEFNITTIINSHDMNSVIEMGENIIFINNGIMA
                     WSGTNKDIYLAENEDLNDFVFASELYKQVKKINQ"
     gene            complement(3751..4491)
                     /locus_tag="PKH58_07325"
     CDS             complement(3751..4491)
                     /locus_tag="PKH58_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX88877.1"
                     /translation="MNIIKDIGLYSMLMYRVFTKPDRGRMFWKQLPKELEKLGLASLP
                     IVIIISVFIGAIMTIQTKLNTENPLLPTYSTGLVTRDTLLLEFSSTIMCLILAGKVGS
                     NIASEIGTMRITEQIDAIEIMGINSANYLILPKITAFILMMPFLVIFSMAIGLFGGYL
                     VGAFSDIITTADYLLGIQYAFIPYYVFYSVVKSVIFAFLISSVAAYYGYNAYGGALDV
                     GKASTNAVVNSSILILFFNLLVTNLMLN"
     assembly_gap    4985..5084
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5085..6680
                     /locus_tag="PKH58_07330"
     CDS             <5085..6680
                     /locus_tag="PKH58_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HNX88878.1"
                     /translation="LTSVSAFAGGLLTNTNQSVHFLRNPARDASTEIDAVYTNPAGVV
                     YLSDGFHISLNNQSAFQTRTITSTFAPFAGYGGNTTKEFNAKTTAPVIPSIQAVYKKD
                     RYAISAGLGVVGGGGKATFNSGLPSLEAPVSMIPGMLTNNKITTSSYSLDQYMEGKQY
                     TFGVQLNGSFRFTEHLSGAVGVRLNMVSNTYQGYLRNIMINPNQPAFGAVFNGSAMVS
                     APTFFTAAATTLNGWAGGASSYVAGLQPIVAGGGGTVLLSNGVSAGLNATQIAQIQGL
                     LGAAGLTPAQIGAINIQTAQATLSAAAPVFTGKAATMNASAAGTADRELNTQQSGMGF
                     APIFSLNYNTDRLNVAVKYEMKSSITLTNNTTVDNTGMYADGAKTPYDIPAYLAAGAE
                     YKIADNWKISGGYHHFFDSDAKMANDKQQYINGGVNEYLLGSEYKIGKKFLISAGGQI
                     SRTAVTDDFQTDLSFGLNSMSFGFGGAVDITENLRLNLACMITGYSDWTKETSNYNAT
                     TLAGKDVYARTSKAFGIGLDYKF"
     gene            complement(6861..7214)
                     /gene="rplS"
                     /locus_tag="PKH58_07335"
     CDS             complement(6861..7214)
                     /gene="rplS"
                     /locus_tag="PKH58_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNX88879.1"
                     /translation="MDLLKVAEQAFAVETEKTLPAFKSGDTITVAYRIKEGNKERIQE
                     FRGDVIKITGSEDKKRFTVRKISGNVGVERIFPMDSPFIESIAINKRGKVRRAKLYYL
                     RKLTGKKARIKERRV"
     gene            complement(7373..10660)
                     /locus_tag="PKH58_07340"
     CDS             complement(7373..10660)
                     /locus_tag="PKH58_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNX88880.1"
                     /translation="MLNIYRASAGSGKTYQLTKEYIRLLFSANGRDNAHRSIMAVTFT
                     NKATDEMKSRILTELYNLYKGKESDYRPELMKEYSLDENEVNLRAGKILFNILHDFSS
                     FSISTIDKFFQQVIRAFAREIGVNGGYNLELDKDNTLLQAVDNLFLELSGEDNKLLLE
                     WMTRFAEEQVEQSASWNIQKEINNLGQEIFKENYQHKAAETSMKMHDRNFLEEYLQKI
                     RKIKSDFENEVSETARNSWNIITSHGLSNDDFSGKKIEGLLKKLQNKNYELSATLLKF
                     ADDVTNCWTKKTPQPTRDAIQSAYDNGLGDNLKKIIRLLSEDIIYYNTANLILKHINT
                     LGIMSDLAMQIKKITSEQNIMLISDTNLLLNKIIDNSDTPFVYERTGLNINHFMIDEF
                     QDTSVLQWKNFKPLISNSIAANNYNMVVGDVKQSIYRWRNSDWKLLDEQVGRDFSADS
                     IKNEHLDTNWRSDKNIIDFNNAFFSIASKLLQVKFNEKFDSSNFHDEALEPLKLKIKH
                     AYHDIDQVISGKAADGHVRFEFIGQDDSEEKWKEKSLQRIPRLLEELTDQGYKPSDVA
                     FLVRKNDQEAEIVQYLLSYKKSEYAREGYSYDIVGNEGLLLSSSASVSFILAVLRLMA
                     NPNDSVQQTILNYEYLRGRKQFSESEAIAACFSSAGNTSEFSALFSDEENDFLSNSRQ
                     NSLYYLIENLISVFEIQQWHNESVFLQAFQDVVFRFVNSKNADLNSFLIWWDSNGDKQ
                     YISTPESQNSFRIMTIHKAKGLDFKVVIIPFCDWPLDAAKTPPLMWCQTDIEPFNEFP
                     LIPVDFSSNLVNSIFQKEYYSELMHQYIDSLNMAYVAFTRARNEMICLAPLPKNGTPM
                     ETAKISSLSELLFSSFLVDDVADSKHIVLSEKYSSDDFLFEIGKPEKAVYKEKPKDAE
                     LRQMNDYPIADSRDRLKIKHNSTEYWLSEQNLTESRLNYGTVMHEILKKIVTRNDEEK
                     AIHEMLVSGKINKNESEYIREEFQKFWNLPETEHWFDESAEILNETTILLPSGENYRP
                     DRVMLKGNKAVIIDYKFGDQILKTHIRQISNYEKIIQQMGFEAESYLCYVSLNKIIRL
                     QESLPNT"
     assembly_gap    10914..11013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11239..12087
                     /locus_tag="PKH58_07345"
     CDS             11239..12087
                     /locus_tag="PKH58_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX88881.1"
                     /translation="MKKVNKEIIRGILNIFSEKPYQIGIAMSGGGIRGMCHAGVLKAL
                     EEFDIRPDIISGVSAGSIVGALYADGYTPDEIAGFFENVEFRKMTKLRIPNGGLFGIG
                     SFEKFLGGKLRAKTFEELKIPLRIVATNLDKGESVVFSSGNLLDAIIASSTFPVLFTP
                     KVINGENYIDGGVFKNFPVSTIRDDCEKVIGINASPLVADKYKMSVLNVAIRSYNFMF
                     KANILHDKELCDYLIEPVDMANYETFETEKSREIFELGYQTARTMIENKLNETKNVNY
                     LINKKQ"
     gene            12186..>12493
                     /locus_tag="PKH58_07350"
     CDS             12186..>12493
                     /locus_tag="PKH58_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX88882.1"
                     /translation="MLSVSERLASLAPSATLAMSQKSNELKAQGIDVINLSVGEPDFN
                     TPDHIKAAAKKAIDDNFSFYSPVPGYPALRKAICEKLKTENGLDYTPEQIVCSNGAKQ
                     S"
BASE COUNT         3538 a         2402 c         2483 g         3870 t
ORIGIN      
        1 atttcagcct gattaataac ggttacaatc cggcttactc ctttataaaa gtttgttttg
       61 tcagcgttta cttcgatctc attgagttga atattttttt ccactgattg tgaaaataca
      121 ttgagtgttg cagcagagat gaataatgtt gtaataattt tcttcattct taattgaaat
      181 taaaacagct gactataagg atatctctta aagtcagctg ttgtatatgt tgattaaatg
      241 acggtctgta ataaaaacag ccggtgagtt tactgtatat agttgttctg taagcttttt
      301 taaaattaaa agatcagtaa aattttcttc ctcctctgta ctgtcgtcag tgtcggtttc
      361 aaagtatgtg tcctgtgttt ctttacctga ttttagcagt gctttttcac aaatatcaaa
      421 ggagatgtgc ccgattgata tttcacgatg caatccggca aaaacagcat aatttttcct
      481 tgtccacctg cgaaagcgga tagtactgta attctttttt aaggcagatg tgtccaaaat
      541 tccaaaatca agttggtcgt ttgtaatcag tatttcgatg gatcaacaaa gttgtagaaa
      601 ttcgccttta ccgtccatac atctgagaga gggagaaaca tacccttacc accgataata
      661 tataaggtat tattggtttt attatgaatg actgaagcat ttttacggta tgtaaagctg
      721 gcaggaagca tattaagact tgtgtcaggc tttgcccacg acagaccttc gtccagcgac
      781 tgtcgcatct gtaccgaggt gtctttcagc tgttcgtagt tatctgtgcc gccgattaat
      841 agcagtttac ttccgtagta acagatgcct gcatccgaaa tggcttcaaa agtgctgtga
      901 ttgttgttca ggtttaccca gtggagcgag tcggttgtcg gatcgttctc tgcagaccag
      961 cgtgtattca gttttttgcc tgaggcgtct tttccaccca caataattat tttttcgcgt
     1021 ccaaggcgtg gggtgaatgt tgttgcggca aagtctgaaa ctggaaatct tgctgataac
     1081 gcattacgta cagtccagtt aattccgtcc gacgaactgg caatattaag ccttttggtg
     1141 gttttattct gtgctacaga ccagtactga tttttgaaat taaataacaa taccagaaaa
     1201 tcgtagttgt tatctccgtt caacaggtgt ttttcccagc ttactccgtt tcgggtggtg
     1261 taaagctcgt tgccgttatg cagcaataaa atctgatcgt tgcaaacaat catgtttctg
     1321 aaaaccgcat tggcaggcag gcctgtcgga ttgctttcgt gtagccatga gctggcatcg
     1381 gtggaagaat acagttgtgt gctgttgccg ttagaggtaa agaaatagta tttattattg
     1441 agcaatactg ctttctgatt gtcagaggca ttttcagtaa tcatttcgac aggatttttc
     1501 caaacatgca ggtaagtttc gacctgatga acattcactt tgatggtgta ttcttttttt
     1561 gtttttccgt cggaagcata attggtcagc ttaaccggtt tcgaaaaatc gtatgcagta
     1621 cttgttgtat aagcagtttc gggaaaggtg tcattgatta ggtagccgtt agtacttgcg
     1681 aatgagaatg aagcataggc actgtccacc cgggtttgat agggaagaga atcgacatta
     1741 taaatcagtc cgttttcgtc gtcgataatg aaaacagcat ccgaaaggtt tggagaactg
     1801 tcattgttct gaaaatacat cgattcaatc gttgcatcat ccgatacgga tacttcggtg
     1861 gtggtattgc aggcaataaa aaaactggtg aaaccaagta gaaataaaag acttttatgc
     1921 tgaagaattt tcatatcgta aatataatga acggataatt gtgtgaaaat aattatattg
     1981 caaagttaaa aacaatttgc agtaatttta acctgatacc gaagttttat tattttttat
     2041 agatgtaatt aatccctatt gcctgaatga tcaaccatta agctccttct gtttctaatt
     2101 caaccattac agggcagtgg tcggaatgaa cggcatcgga taaaatggct gccgatttca
     2161 ggctggattt tacaggttgg cttacccagt gataatcaat acgccaaccc atgttgcgag
     2221 accgggcatc tgtccggaaa ctccaccagc tgtatttctc tgcacttttg tcgaattcgc
     2281 gaaaagaatc gaccattcct gttaattgaa attcatctag ccaggctctt tcttcaggta
     2341 gaaatccgga aacacctgcc tgacgctcgg gatgattgat gtcgataggc ttatgacaaa
     2401 tgttgtaatc tccacaaaca accagatttg gacgtgtttt acgtaaatcg gaaatatatt
     2461 gactgaaatc gttcaggaat tccattttga actgttgtcg ctcaccaccc gacgaacccg
     2521 acgggatata cacgcacact actgtcagat cgtcgaaatc agcacggagt acacgacctt
     2581 ccgaatcata tttatgatta tccatgccaa taacaacatt gtcaggtgga attttagtga
     2641 aaattgctac tccgctgtaa cctttcttaa gggctgaatg gatataacag gtgtaaccat
     2701 actgattgaa tatcgaagtg tcaatttgct ccggttgtgc tttggtttcc tgcaggcaca
     2761 gaacatcggg atttgtatct ttcagccagc tttccagtcc cttggacaat gctgcacgga
     2821 ttccgttaac gttatatgaa ataattttca tcaataattt agtaaaatag gaatataaat
     2881 cgtggatgtt tacagcttaa tctttaatct ttaatcctcc aatcctccaa tcttcaaatc
     2941 ttcaaatttt caaattttca aattttcaaa ttttcaaatc ttcttactga ttaatctttt
     3001 taacctgttt gtacagttcg gatgcaaata caaaatcgtt cagatcttca ttctcagcca
     3061 gatagatatc tttgttagtc cctgaccagg ccattatccc gttgttgata aaaataatat
     3121 tttctcccat ttcgattacc gaattcatgt cgtgcgagtt aattattgtt gtgatattga
     3181 attcgtgagt aatttcgtaa atcagctggt cgatgatcag tgatgttttc ggatcaagtc
     3241 cggagttagg ttcgtcgcaa aataaatatt tgggattaag tgcaatcgct cgtgcaatgg
     3301 ctacacgttt ctgcataccg ccgcttattt cggaaggaaa aaggtggtag gaatgctcgt
     3361 tcagattaac tctgtcaaga caaaattttg tacggttttc catttccttt ctggaggagg
     3421 aagagaacat ctctagcgga tacatcacat tttccatcac agtcattgag tcgaacagcg
     3481 ctgaaccctg aaaaagcatc ccaacttcac gtctcaaccg acggatatct ctcattctca
     3541 tatccggcag atttctaccg tcatactcaa ttttgccaga gtccacgcga tgcagaccga
     3601 taatactttt catcagtacg gttttacctg aaccgctctg accaataatc atattgcatt
     3661 tcccggtttc aaacacggct gaaatgtctt tcaaaacctg ttcaccgtca aatgacttaa
     3721 ataaatgaga aacttcaatc ataatacttt ctagtttagc atcaggttgg taaccagcag
     3781 gttgaaaaac agtattaaaa tgctgctgtt aactactgca tttgtactgg ctttacccac
     3841 atcaagtgca cctccgtaag cattatagcc ataatatgca gcaacagatg aaatgaggaa
     3901 tgcaaaaatg accgatttca caacagaata aaacacataa taagggataa acgcatactg
     3961 aatgcccagc agataatcag ccgtagtgat tatgtcgctg aaagcaccta ccagataacc
     4021 tccgaagagt ccgattgcca tgctaaaaat gaccagaaat ggcatcatta atataaaagc
     4081 tgttatcttc ggtagaatca ggtaattggc tgaattgata cccataattt cgattgcgtc
     4141 aatctgttcg gttattcgca ttgtgccgat ttcggaagca atgttggaac ctacttttcc
     4201 ggccagaatg agacacatga tggtggacga gaactcgagc agcaatgtgt cacgtgtcac
     4261 taaacccgta ctgtatgtcg gcaggagagg attttcagta ttcagtttcg tctgtatcgt
     4321 catgatagca ccaataaata cggaaataat gatgacaatg gggagtgaag ctagcccgag
     4381 tttttcaagt tcttttggaa gctgtttcca aaacatccgt ccacgatcag gcttggtaaa
     4441 aacccgatac ataagcatgg agtataatcc gatatctttt atgatattca tattgacgta
     4501 attttatcaa ttttttcagg gtgcaaatat acgatttttt tatccttcgg gtagtaacta
     4561 caaaatataa gattatacat aggtggtttt tggagaaata cattttgtct aaaatcactt
     4621 gttttgaatc ttaatacaga ttttgaagta aatattaact tataattaca aatgtctgta
     4681 tcgtaactgc cagatagctg attgacaatg tagtatggtt ctatattaat tcggaaatta
     4741 tccacaatat gcacaaatgg tgtaaatatg tgcatgataa ttttctgaaa ttgcacaaat
     4801 tgatgtaaaa tgtgtaattt tgttccctgt aatttttcat ctgttgtatt cgaaccaaca
     4861 gatggttatc attcattcta aaaaagctta tttatgatta aaaattttat tattggtgga
     4921 tttttgtttc tgacatcggt gtcagctttt gcgggtggtt tgctcacaaa caccaatcaa
     4981 agtgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctgaca tcggtgtcag
     5101 cttttgcggg tggtttgctc acaaacacca atcaaagtgt tcatttcctt cgaaacccgg
     5161 cacgcgatgc gtccactgaa attgatgctg tatataccaa tcctgcagga gtggtttatt
     5221 tgtccgatgg ctttcatatt tcactcaata atcagagtgc attccagaca cgaacaataa
     5281 cttccacatt tgcacccttt gccggttatg gtggtaacac tacaaaagag ttcaatgcca
     5341 aaacaactgc tccggttatt ccaagtattc aagctgtgta taaaaaagac cgttatgcta
     5401 tttctgcagg attaggtgtg gtaggaggtg gtggaaaagc cacttttaac agtgggttgc
     5461 cttcgctcga agcgcctgtt tctatgatac ccggaatgct taccaacaat aaaataacga
     5521 cctcttccta ttcgctcgat caatatatgg agggaaaaca atacactttt ggcgttcaac
     5581 ttaatggctc ctttcggttt accgaacacc tgtcgggagc cgttggcgtt cgtctgaata
     5641 tggtcagcaa tacttaccag ggttatctga gaaatataat gattaatccc aatcaacctg
     5701 catttggagc agtattcaat ggctcggcga tggtttctgc accaacattt tttacagctg
     5761 cagcaaccac ccttaatggt tgggctggcg gagcaagttc ttatgttgca ggtctgcaac
     5821 ctattgttgc tggtggtgga ggtactgtgc tgttgtcgaa tggagtatct gccggattga
     5881 atgctactca aatagcacaa attcagggtt tgcttggagc tgccggacta acacctgctc
     5941 agattggagc aatcaatatt caaacagcac aggcaacctt gtcggcagca gctccggttt
     6001 tcaccggaaa ggcagcaaca atgaatgcaa gtgctgccgg tacagccgac agagagttga
     6061 atacacaaca gtcgggtatg gggtttgctc cgatattcag tttgaattac aacactgacc
     6121 ggttgaacgt agctgttaaa tacgaaatga agagttcaat tacactgact aataatacca
     6181 ccgttgacaa tacaggtatg tatgccgatg gagcaaaaac tccttatgac atacctgcat
     6241 atctggctgc aggagcagaa tataaaattg ccgataactg gaaaatctca ggcggttatc
     6301 atcatttttt cgattcggac gcaaaaatgg caaatgataa gcaacagtat attaatggcg
     6361 gagtaaatga gtatttgctt ggttcggaat ataaaatcgg taaaaaattt ctgattagtg
     6421 ccggtggtca aatcagccgt acagctgtta ctgacgattt tcaaacggat ttgagttttg
     6481 gtcttaactc catgtcgttt ggttttgggg gagctgtcga tatcactgaa aatctgcgcc
     6541 tcaatttggc atgtatgatt accggttatt ctgactggac taaagaaact tcgaactaca
     6601 acgctacaac tctggcaggt aaagatgttt atgcccggac aagtaaagca tttggtatcg
     6661 gacttgatta taaattctga aaaaaatcac acacttcaaa acaattttca attcaatctg
     6721 atctttaatt gcaacggaac aatcctgccg ggttgttccg ttactttttc acctgaatat
     6781 gaaagtatca gtgattaaat aaaaaaaaga gaaactcatt ttgaatttct ctttttttat
     6841 ttcgctggtc gaagattgac ttacacgcgt ctttctttga tacgtgcttt tttaccggta
     6901 agtttacgca ggtaatacag tttagcgcgg cgaactttac ctcttttgtt gatggcaata
     6961 ctttcgatga acggagagtc cattgggaaa atacgttcta cgcctacatt acctgaaatt
     7021 ttacgaacgg taaagcgttt tttgtcttcg cttccggtaa ttttaattac atcgcctctg
     7081 aattcctgaa tacgttcttt gttaccttcc ttgatacgat aagcaactgt aatcgtgtcg
     7141 ccgcttttga atgcaggcag ggttttctct gtttcaactg caaaggcttg ttctgcaact
     7201 tttaataaat ccatgatttc ctgattttta agttcatact ttgcgcaata ttcacagacc
     7261 tcatttattc tgtcagagat tacgctgaat ttggacgaca aaagtacagc ttttttttgg
     7321 taatacaata tgcttgtgaa aaattattat taaatacccg aagtttttct gattatgtat
     7381 tcggtagcga ttcttgtagt ctgatgattt tattcaaact gacgtaacaa aggtagcttt
     7441 ccgcttcaaa acccatttgc tgaataattt tctcgtaatt acttatctgg cggatatgtg
     7501 tttttaaaat ctgatctccg aatttataat caataatcac cgctttattt cctttcagca
     7561 tcacccggtc gggccggtaa ttctctcccg aaggcaggag aatagtagtt tcgttcagta
     7621 tttcggcgct ctcatcaaac cagtgttcag tttccggcaa attccagaat ttctgaaatt
     7681 cttcacgtat gtattcactt tcattcttat ttatttttcc tgaaacaagc atttcatgaa
     7741 tggctttttc ttcatcgtta cgggtaacaa tttttttcag aatttcgtgc atcacagtgc
     7801 cgtagttcag tcggctttcg gtcagatttt gttcagacaa ccagtattcc gtgctgttat
     7861 gttttatttt cagccggtca cggctgtctg ctattgggta gtcgttcatc tgtctgagtt
     7921 cggcatcttt cggtttttcc ttataaacag ctttttcagg cttgccaatt tcaaataaaa
     7981 aatcatcaga agaatatttt tcggataata caatatgttt tgaatcagca acatcatcta
     8041 ccagaaaact tgaaaacaga agttcggaca gtgagctgat tttagcagtt tccatcggag
     8101 ttccgttttt tggcagcggc gccaggcaaa tcatttcgtt gcgggcacgc gtaaatgcca
     8161 cgtatgccat gttcaggctg tcaatgtact gatgcatcag ttcagagtag tattctttct
     8221 gaaaaataga gttaacaaga ttcgacgaga aatcgacggg gataagcgga aactcattga
     8281 aaggctcgat atcggtctgg caccacatca gtggcggagt ttttgcagca tcgagtggcc
     8341 agtcgcaaaa aggaatgata acaaccttga aatcgagccc cttggctttg tggatagtca
     8401 tgatacggaa cgaattctga ctttcgggtg tggagatgta ctgtttatca ccattgctat
     8461 cccaccatat caggaagctg ttcaaatcgg cattttttga gtttacaaac ctgaaaacaa
     8521 catcctgaaa agcctggagg aaaacacttt cattatgcca ttgctgaatt tcgaaaactg
     8581 aaatcaggtt ctcaatcaga tagtacaatg aattttgtcg ggagttggat agaaaatcat
     8641 tttcttcatc ggagaacagg gctgaaaact ccgaagtatt tccggcagaa gagaaacatg
     8701 ctgctattgc ctcgctttcg gagaattgtt tccggccgcg gagatattcg taattcagaa
     8761 tggtttgctg aacactgtcg tttggatttg ccatcagcct gagcacggct aagataaaac
     8821 tcactgaagc cgatgaagac agcagaagac cttcgttgcc tacgatgtcg tagctgtaac
     8881 cttcacgtgc atattccgat tttttataag acaataaata ctgaacaatt tcagcctcct
     8941 gatcattttt acgaaccaga aaagctacat ccgaaggttt ataaccctga tctgtcagtt
     9001 cttcgagcaa ccggggaatt cgctgcaagc ttttttcttt ccatttttct tcagaatcat
     9061 cctgcccgat aaattcaaac ctgacatgcc catcagccgc cttccccgaa atcacctgat
     9121 caatatcatg atatgcatgt tttattttca gtttcagcgg ctccagagct tcatcgtgga
     9181 agttcgagct gtcaaacttt tcattgaact ttacctgaag cagtttcgat gctattgaaa
     9241 aaaaggcatt attgaaatca atgatgtttt tatcgctccg ccagttggta tcaagatgtt
     9301 cattttttat gctgtccgcc gagaaatcac gtccaacctg ttcgtccagc agtttccagt
     9361 ccgagtttcg ccagcggtag atgctttgct ttacatcgcc cactaccatg ttatagttgt
     9421 ttgcagctat gctgttggat atcagtggtt tgaagttttt ccactgcaat accgaagtgt
     9481 cctgaaactc atcaatcata aaatggttga tattaaggcc ggtgcgctcg taaacaaagg
     9541 gagtgtcgct gttgtcgatg attttgttaa gcagcaggtt tgtatccgaa atcagcataa
     9601 tgttttgttc gctggtaatc ttttttatct gcatcgccag atcggacatg atacccagcg
     9661 tgttgatgtg cttgagtatc aggttggctg tgttgtaata tataatgtct tcggataaga
     9721 gtctgattat ttttttaagg ttatctccaa gaccattgtc gtatgccgac tgaatggcat
     9781 ctcttgttgg ttgaggagtt tttttggtcc agcagttggt cacatcatcc gcaaatttca
     9841 acaaggttgc actcagttcg taatttttat tctgtaattt ttttaataag ccttcaattt
     9901 tctttccgct gaaatcatca ttacttaagc cgtgtgaagt tattatattc catgaatttc
     9961 gggcggtttc gctcacttca ttctcaaaat cagattttat ttttctgatt ttttgcagat
    10021 attcttccag aaaatttctg tcatgcattt tcatgcttgt ttccgctgcc ttatgctgat
    10081 agttttcctt gaaaatttcc tgaccgagat tgttgatttc cttttgaatg ttccagcttg
    10141 cagattgttc cacctgctct tcggcaaatc gtgtcatcca ttcgagcaag agtttgttgt
    10201 cttcacccga aagttccaga aaaagattat ctacagcctg cagcagtgta ttgtctttat
    10261 caagttccag attgtaacct ccgtttacac ctatttcgcg ggcaaaggcg cgtatcacct
    10321 gctgaaaaaa cttgtcgatt gtactgatgg aaaaggatga aaagtcgtgc agaatgttga
    10381 acaggatttt accagcccgt aggttcactt cgttttcatc cagagagtat tccttcatta
    10441 gttccggtct atagtccgat tctttacctt tataaagatt gtagagttcc gtcaggatac
    10501 gcgatttcat ctcgtcggta gctttattgg taaacgttac cgccattata ctgcgatgtg
    10561 cattatcgcg gccattagcc gaaaaaagca aacggatata ttctttggtt agctggtagg
    10621 tttttcctga accggcagaa gcgcgataga tgtttaacat ataaaccccc taacccccct
    10681 aaaggggaaa ttttagaata gttagaacct ggacgatttg atttttagct ctaagcaaca
    10741 tgcagtttta aaccgtatga tgcatagata ttaccagaaa aagtcttttt gcaaaaatag
    10801 gaattataat tgatactaaa tggtactggt tttaccttta atgaaaagtt agcataagtt
    10861 tgatggcttt ttttgcaccc aacaaagtct cccctgaggg ggatttaggg ggcnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagtatgc ctccataact cagtggcaat
    11041 ccgggcagta caggaagaac agagccgata atgcctccga caatacacac aatccccaat
    11101 ataataagta atgcatccat atggaatgta ttaaattaat ctcttacaaa gataagaata
    11161 aactcacgga tgaaaagaaa aatgtataaa tttgtgaggt tgaaaaataa ctaatttcac
    11221 ttcaataaca gacatacaat gaaaaaggta aataaggaaa ttatccgcgg aatactaaat
    11281 atattctccg aaaaaccgta ccagataggg attgcaatga gtggtggtgg catccggggt
    11341 atgtgtcacg ccggtgtgct gaaagcgctc gaagaatttg atatcaggcc ggatataata
    11401 tcgggtgtaa gtgcaggttc aatagtcgga gcgctctatg ccgacggata tacccccgat
    11461 gaaattgctg gatttttcga aaatgttgaa ttcagaaaaa tgacaaaact cagaattccg
    11521 aatggcggac tcttcggtat aggcagtttc gagaaattcc tgggaggaaa gttgagggca
    11581 aagacatttg aagaactgaa aataccattg cgtatcgtgg caacaaatct ggataaagga
    11641 gaaagtgtgg ttttttcaag cggtaatctg ctggatgcta tcattgcctc gtccactttt
    11701 cctgtacttt ttacccccaa agtcattaac ggagaaaatt acattgatgg tggtgtgttt
    11761 aaaaactttc cggtcagtac cattcgtgac gattgcgaaa aagtaatcgg cattaatgct
    11821 agtccgttgg tagccgataa gtacaaaatg tcggtcctga atgttgccat ccgttcctat
    11881 aactttatgt tcaaagctaa tattctgcac gataaagagt tatgtgatta tctgatagaa
    11941 cctgtcgata tggccaatta tgaaacattt gaaacagaga aaagccgcga gattttcgaa
    12001 cttggatatc agacggcccg cacaatgatt gaaaataaac tgaacgaaac aaaaaatgta
    12061 aattatctga ttaacaaaaa acaataattt aataaattta atgtgttgaa ttgagaaaaa
    12121 tatgtgcaaa atccgattaa tttgattaaa tttgcactaa attataaaca aaaaacattt
    12181 taattatgct ttcagtatca gaacgtttag cttcgctcgc accatcggca actcttgcca
    12241 tgtcacagaa gagcaacgag cttaaggcac agggaattga tgtgattaat ctgagtgtcg
    12301 gtgaacccga tttcaataca ccggatcata taaaagctgc tgccaaaaaa gcgattgatg
    12361 acaatttctc gttttactct cccgtgcccg gatatccggc gctgcgaaaa gctatctgcg
    12421 agaaacttaa aaccgaaaac ggtctggact acaccccgga acaaattgtt tgttcaaacg
    12481 gtgcaaaaca atc
//
LOCUS       DAOPYB010000077        12430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5687_length_12430_cov_9.950222, whole genome shotgun sequence.
ACCESSION   DAOPYB010000077 DAOPYB010000000
VERSION     DAOPYB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12430
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5687_length_12430_cov_9.950222"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKH58_07355"
     CDS             <1..445
                     /locus_tag="PKH58_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX88883.1"
                     /translation="FSLTLNRDDAEDLLQDTTLKVLDNQDKFTSNVNFKGWVLTIMKN
                     IFINNYRKIVRNQTIFDRTDDLHHLNLPQNSGFETPEGAFSFGEISYSIDSFADEYRI
                     PFSMHVQGYKYEEIAQKMDLPIGTVKSRIFLARKRLQEQLKDYKF"
     gene            complement(521..763)
                     /locus_tag="PKH58_07360"
     CDS             complement(521..763)
                     /locus_tag="PKH58_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNX88884.1"
                     /translation="MGIIWFIVIGALAGYLAGKLMKGGGFGLVVNLVVGIIGGVLGGW
                     IFGIFRIDAGEGLLGSLVTSTVGAVVFLWLLSKFKK"
     gene            986..2635
                     /locus_tag="PKH58_07365"
     CDS             986..2635
                     /locus_tag="PKH58_07365"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNX88885.1"
                     /translation="MIKKYLLTALSVILITFGAHPCTNFLVGKSATTDGSTFISYSAD
                     SYFLFGALYHYPAATYPRGTMLDINEWDTGKYLGKIKQAEQTYSVIGNMNEHQVSIGE
                     TTYGGREELVDTTAIMDYGSLIYIALQRSRTAREAIKVMTDLVAEYGYYSSGESFSIA
                     DPNEVWIMEMIGKGPGRKGAVWVALRIPDDCVSAHANQARITTIPFKDKNNCMYSKDV
                     VSFAREKGYFTGKDEEFSFSDTYAPLDYSALRICESRVWSFFRKVKPDMDKYISYVKG
                     ETKERMPLWIKPDRKLSAEDMKSYMRDQYEDTELDMTKGCAAGPFGSKLRHSPLTFTV
                     DSVEYMHERPIATQQTGFTFVAQMRGWLPDYTGGILWFGVDDAASNVYVPVYCCTNKV
                     PQCFDEKNGSLLQYSPTSAFWTFNAVANFAYGKYSYLMSDIRKLQSELEHGFQQMTPA
                     VDAKALALGETAGRKYLTDYSNQAAEKTTAEWKKLGEYLLVKYMDGNIKKEENGKFLQ
                     NEHGIPPGIIRAGYPTEFQRKIAKENPGLRVKAAEEIKNRK"
     gene            complement(2725..2922)
                     /locus_tag="PKH58_07370"
     CDS             complement(2725..2922)
                     /locus_tag="PKH58_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNX88886.1"
                     /translation="MFTEKIGTNAGLVWAALENGELNVKAVKKATKLTEKDLNLAFGW
                     LAREGKVAFREEEGEVFIALV"
     gene            complement(3078..3614)
                     /locus_tag="PKH58_07375"
     CDS             complement(3078..3614)
                     /locus_tag="PKH58_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNX88887.1"
                     /translation="MDPLKIIEKYYDKNSNLYDILVRHSIKVCEKALMIADKHPELHV
                     DRKFIKEASMLHDIGIFKTNAPDIECYGTHEYIEHGYLGSEIVFNEGYPRHALVCERH
                     TGMGLTLEWIIESNLPVPHRDMMPVSIEEQIICYADKFYSKTKFDNEQSVEKILSKLS
                     KYDENQARKFIEWNERFG"
     gene            3743..4330
                     /locus_tag="PKH58_07380"
     CDS             3743..4330
                     /locus_tag="PKH58_07380"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNX88888.1"
                     /translation="MANVEKNNEISDCWWSPGDPLMKEYHDMEWGVPLHDDRKLFEFM
                     VLDAFQAGLSWKTILYKREGFRLAFDNFDVEKIALYDEQKVQELMQNASIIRNQAKIR
                     ATITNAKQFISVQQEFGSFDAYIWQFTGGTTIHHHCNVISDIPARSPESDAMSRDLQK
                     RGFKFVGSTICYAFMQAAGMINDHLNGCSRNPLKA"
     gene            4327..6783
                     /gene="priA"
                     /locus_tag="PKH58_07385"
     CDS             4327..6783
                     /gene="priA"
                     /locus_tag="PKH58_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNX88889.1"
                     /translation="MKYVDVIVPLPLSSTFTYAVPEAMTDTVQIGMRVVVPFGKKKMY
                     TAIVCMIHSNEPILYEAKEIICTLDNEPILRYPQLKFWEWMSNYYQSAIGDVYQAAVP
                     AGLKLESETQVRVNPDYESESSLTEKEYKLLDALSDGKIQSVSDLNKLTGMRDTLPLL
                     KTLMEKGAVEVSEELTEKFKPKTESYIKLSAYASDENNLRDIFDKLTRAKKQLEVLMT
                     YIELSRCLSKTNIREVSRKELLEKTNAATANISGLIERGVFEAYSKETGRLDYSDVNT
                     EEMNPLNEFQQEAMNNINRQFTEKQVVLLHGITSSGKTEIYIHLIDKVIATGKQVLYL
                     VPEIALTTQLTSRLKRIFGKRLGVYHSKFSDAERVEIWNNVLLNKTYDVVIGVRSSVF
                     LPFRQLGLVIVDEEHENSYKQYDPAPRYNARNAAIVLASMHGAKTLLGSATPAIESYY
                     NSQNGKYGYVALNQRYQEIELPNILIADTKEAYRKKEMEGHLTPLLSDKITKALNNNE
                     QIILFQNRRGYAPFLECKGCAYVPKCKNCDVSLTVHKFSNKLTCHYCGYTENIPSVCP
                     VCKTPGLVDKGFGTEKIEQEIQEAFPGAKVARMDLDTTRSKKGYDKIISDFELGKVDI
                     LVGTQMVTKGLDFERVSLVGILNADNLLNFPDFRAHERAYQLMAQVSGRAGRKNKRGT
                     VVLQTSNPTHPVIGQVLRNDYTAMFNTQCDERRQFSYPPYFRLIEITLRHRDSSTLNH
                     AAEEFGVNMRKIFGTRVLGPTIPPVSRIQNMYIKQILLKIETTASAEKAKQILGEITS
                     ALLSQNPYKAVRISIDVDPM"
     gene            7006..8454
                     /locus_tag="PKH58_07390"
     CDS             7006..8454
                     /locus_tag="PKH58_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNX88890.1"
                     /translation="MQEIKFYSGENIPLELHKVRIVQKLNLVPIERRLQALFEGGFNT
                     FRLSTRDVFLDMLTDSGTNAMSDNQLSAMMRADDAYAGSQSFERLQKAVEDVLDKKYL
                     LPVHQGRAAENILSVAYVHKGSLVPMNYHFTTAMAHITDKGGNIAELLYDEAYVINSD
                     HPFKGNMNIEKLEETINYHGPKNIPFIRMEASTNLIGGQPFSVQNLRDVRAIADKYKI
                     RLILDCSLLGENAFLVLQREEEFKNSNMETVIKTMTGLADICYFSARKLSSSRGGGIC
                     TDSLEIFKELEAFIPLYEGFLTYGGMSIREVEAMAVGLYETLDETVISQSPSFIKYLV
                     EQLDKKNIPVIKPAGVLGVHLDAMQICAHIPQTQYPAGCLAAALFLISGIRGMERGSI
                     SNQRDEYGNETYADMELLRLAMPRRVFTLSQVKYVEDRVTWLYDNRHLIGGLKFVYEP
                     PVLRFFMGGLEPVNDWPEKLMAKFKEDFGESL"
     gene            8563..8967
                     /locus_tag="PKH58_07395"
     CDS             8563..8967
                     /locus_tag="PKH58_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88891.1"
                     /translation="MITHLLNHRISPDAITELRRNEVFVFGSNLDGLHYGGAARIAHE
                     KFGAVWGQGTGLHGKSYAIPTMHGGVDQIRPYVDDFIAFAASHPQLTFLVTRVGCGIA
                     GFTDKEIAPLFAQATGCNNIFLPESFWNVLRE"
     gene            complement(8977..9972)
                     /locus_tag="PKH58_07400"
     CDS             complement(8977..9972)
                     /locus_tag="PKH58_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX88892.1"
                     /translation="MKQLEKVIWGVIGAGNVCEVKSVPGMYKTEHSEVKMIMRRTADK
                     AADFAHRHGISQWTTEVDEILNDPDINIIYISTPPDSHCELTLRAAAAGKAVYVEKPM
                     ANSYEECISMIEACKAADVPLFVAYYRRALPGFNKLREIITAGGIGEIRFVEIEMWRA
                     PRSYDINTEGNWRVFPEISGGGHFHDLAAHQLDYLDSVFGPVVKAFGVTANQAGMYPA
                     DDIVSASMLFESGVVGSGTWCFATDKASEKETITITGSKGHLVFNTFGSPMKIQITTA
                     DGTETVEVPHPEHIQQPLIQTIVNQLRGVGICPSTGDTGIRTTKVMTEITRKDGQ"
     gene            10122..11090
                     /locus_tag="PKH58_07405"
     CDS             10122..11090
                     /locus_tag="PKH58_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNX88893.1"
                     /translation="MKTKEEVFSGFKLAGIPALLLNFIFLVGIVLLFIYSVSSLSQSI
                     IGVILIITCILLFICNMVAWGGFMQIEPNEARVMIFFGKYNGTVKENGFFWVNPFYAK
                     KKLTLRARNLDAEPIKVNDKAGNPVMIGMVVVWRVVDTYKASFDVDNASMTSGAPGTA
                     TDNVNAKMKAYENFVKIQSDAALRKVAGMYAYDHNTAGDDSSLTLRAGGEEMNEILEN
                     QINERLRIAGIEVIEARINYIAYAPEIAAVMLRRQQADAIIAAREKIVEGAVGMVKMA
                     LDKLSKDGIIELDDEKKAAMVSNLLVVLCGESNAQPVINSGTLYNG"
     gene            11098..11268
                     /locus_tag="PKH58_07410"
     CDS             11098..11268
                     /locus_tag="PKH58_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNX88894.1"
                     /translation="MPADNKQKNFVLRIDSELMEAIEKWAADEFRSTNGQILYLLDQA
                     LSKSGRKPKKKT"
     gene            11553..11930
                     /locus_tag="PKH58_07415"
     CDS             11553..11930
                     /locus_tag="PKH58_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNX88895.1"
                     /translation="MSSEAIILLGSNTNRNLLTEAAALLTRDFEIKACSGIAETGAVG
                     SHYLSPFYNQAILLHSLHNRDEAVRIFKETEKKLGRTPQSKHQGIVPMDIDLVVWNGE
                     VCHGDYFRYDFVRACVNEIWAKE"
     gene            12018..>12430
                     /locus_tag="PKH58_07420"
     CDS             12018..>12430
                     /locus_tag="PKH58_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HNX88896.1"
                     /translation="MKHFPPSELLINDDGSIFHLHLKPEQLADKIILVGDPGRVALVA
                     SYFDEQECSVSSREFNTITGTYKGKRITVVSTGIGTDNIDIVVNELDALANIDLNNRT
                     EKTEFRQLTLVRIGTSGGMQPDIPLGSFLISEKSIG"
BASE COUNT         3773 a         2849 c         2598 g         3210 t
ORIGIN      
        1 attctcatta acgcttaatc gtgatgatgc cgaggactta ctgcaagaca ccacacttaa
       61 ggtgctggac aatcaggaca agtttacgag taatgtaaat ttcaaaggtt gggttctgac
      121 gattatgaaa aacattttca ttaacaatta tcgcaagatc gttcgcaacc agacaatttt
      181 tgacagaaca gacgatttac atcatttgaa tttgccgcag aattccggtt ttgagacccc
      241 tgaaggtgct ttctcctttg gagaaatcag ctacagcatt gattcattcg cagatgaata
      301 ccgcattcct ttttccatgc atgtacaggg atacaagtat gaagaaattg cccaaaagat
      361 ggatttaccc atcggcaccg tgaaaagccg catcttcctt gcccgcaaaa ggctacagga
      421 acaactgaaa gattataagt tctgacaact aaagaaatgt caataaaaaa accttcaaat
      481 aacattgaag gcttttttgt ttcttaaatc tccaaatgga ttacttcttg aatttcgaaa
      541 gtaaccacaa aaacaccact gctcctacgg ttgaagtaac caaacttcct aacaagcctt
      601 caccggcgtc aatacggaaa atgccaaaaa tccagccgcc aaggacacct ccgattattc
      661 ccaccaccag gttaacgacc aatccgaaac cgccgccttt cattaatttc ccggccagat
      721 aaccggccag cgctccgatt acaataaacc atattattcc cataccccaa ttctttttat
      781 tatcattgtt aaaattaaaa tacgtgacaa atataatcat tcaattaaat tatcgtatcg
      841 tgctgaagtg aatttattta acaacaattc gggttcgatt attttttaac attgaaataa
      901 tatactattt ttgctctccg atgtaaagtt tacgagaaaa ttcatctttt acacataaca
      961 gataactgta aaaacaacat actctatgat taaaaaatat cttctgacag cattgtctgt
     1021 cattctgatt acttttggtg cacatccatg caccaatttt cttgtcggca aatccgcaac
     1081 gacagatgga tcgactttta tatcctattc ggcggattct tatttcctgt tcggcgcatt
     1141 gtatcattat cccgcagcca cttaccccag ggggaccatg cttgatatta acgaatggga
     1201 caccggtaaa tacctgggta aaatcaaaca ggccgaacaa acatattccg ttatcggcaa
     1261 catgaacgaa caccaggtga gtatcggtga aacgacctat ggcggacgcg aagaactggt
     1321 tgatacaacg gcaataatgg attacggaag cctgatatat attgccctgc agcggtcgcg
     1381 tactgcccgc gaagctatca aagtcatgac cgacctggta gctgaatacg gctactacag
     1441 ttccggcgaa tcattctcaa tagccgaccc caatgaagtc tggatcatgg aaatgatcgg
     1501 aaaaggtcca ggcaggaaag gagctgtgtg ggttgccctg cgtatccccg acgactgcgt
     1561 atcggcacat gccaatcagg cgcgaatcac cactattccg tttaaagata aaaataactg
     1621 tatgtattcg aaagacgtgg tatcctttgc ccgcgaaaag ggatatttta caggaaagga
     1681 tgaagaattc agtttctccg atacgtatgc gcccctcgat tattcagcgc tgcgcatatg
     1741 cgaatcgcgt gtatggtctt tcttccgcaa ggtgaaacct gatatggaca aatatatcag
     1801 ctatgtgaaa ggtgaaacaa aagaacgaat gcctctctgg atcaagcctg accgcaagct
     1861 cagcgccgaa gacatgaaga gttatatgcg cgaccaatac gaagacaccg agttggacat
     1921 gaccaaaggt tgtgccgcag gtcctttcgg gagcaaacta cgtcacagcc cgctcacttt
     1981 tacggttgac agtgtggaat atatgcacga acgtccgatt gccacccaac agaccggctt
     2041 tactttcgta gctcagatgc gcggctggct gcccgattat acgggaggaa ttctgtggtt
     2101 tggagtggac gatgccgcca gcaacgttta tgttcctgtg tattgctgta ccaacaaagt
     2161 tccgcaatgt ttcgatgaaa agaacggtag cctgctgcaa tactctccca cctctgcttt
     2221 ttggacattc aatgcggtag ctaattttgc atacggaaaa tactcttatc tgatgagtga
     2281 catcagaaaa ttgcagtcag aacttgaaca cggatttcag caaatgaccc cggccgtaga
     2341 tgccaaagcc ctggcactcg gagaaactgc aggacgcaaa tacctgactg attacagcaa
     2401 ccaggctgcc gaaaagacaa cagccgaatg gaaaaagcta ggagaatacc tgcttgtaaa
     2461 atacatggat ggaaatatca aaaaagaaga aaacggcaaa tttcttcaga atgaacacgg
     2521 tattcctccc ggaattatac gtgccggcta tccaaccgaa ttccagcgca agatagccaa
     2581 agaaaatccg ggcctgagag taaaagcggc tgaagaaatc aaaaacagaa agtaaattct
     2641 gaaaaacaga attcttaaat tacagacaaa acaaaagcgg gaaaatcaga tttttccgct
     2701 tttattttta agattatcaa tttcttacac taacgcgata aacacttcgc cttcctcttc
     2761 tctgaaagcg actttacctt cgcgggccaa ccagccaaat gccaaattca agtctttttc
     2821 tgttagtttg gtggctttct taactgcctt aacattcaat tcgccatttt ctagagcagc
     2881 ccaaaccaaa cctgcgtttg ttccaatttt ttctgtaaac ataattgtag ttttaataat
     2941 tcgaatttat tttacgggac aaaaataata aataatatgt tataaaacat aattttcaat
     3001 aaacgaacga taaaaaagcc ctcatttacg cttatttaag ttaatattaa ctattcaact
     3061 atatacttta ttatcttcta cccaaacctt tcattccatt ctataaattt ccttgcctga
     3121 ttctcgtcat attttgatag cttgctcaat atcttttcaa cgctctgttc gttgtcaaat
     3181 tttgttttcg aataaaactt gtcggcatag catataatct gctcttcaat actcacaggc
     3241 atcatatccc ggtgcggaac cggaaggttg ctttctatga tccactccag agtgagaccc
     3301 ataccggtat gacgttcaca aaccaacgca tgccgggggt atccctcatt aaaaacaatt
     3361 tctgagccca gataaccgtg ttcaatatat tcatgtgtac catagcattc aatatccggc
     3421 gcatttgttt taaaaatccc gatgtcgtgc agcatcgaag cttcctttat aaactttcgg
     3481 tcaacatgca gttcgggatg cttatcggca atcatcaggg ctttttcaca gacttttata
     3541 ctgtgtctga ccagtatgtc gtacagattg gaattcttat cgtaatattt ttcgataatt
     3601 tttaaagggt ccattttcag tgattaaaca gtaatacaat ttatttactt ctcatattca
     3661 actaaaatac tttttggtaa ttacagacta aaatataaaa ttcaatctta atttgtattt
     3721 ttgcacaaag aagcattatg tgatggcaaa tgtagaaaaa aataacgaaa tatctgactg
     3781 ctggtggagc cccggcgacc cgctgatgaa ggaatatcac gacatggaat ggggtgtacc
     3841 cttacatgac gaccgtaaac tgtttgaatt catggtactt gacgcgttcc aggccggact
     3901 gagctggaaa acaatattgt ataaacgtga aggtttccga ctggcatttg ataattttga
     3961 tgttgagaaa atagcgcttt atgacgaaca gaaagtgcag gagcttatgc agaatgcctc
     4021 aatcatccgt aatcaggcca agatccgggc tacaatcacc aacgcgaaac aatttatcag
     4081 tgttcagcag gaatttggct catttgatgc ctatatctgg cagtttacag gcggaacaac
     4141 catccatcat cactgcaatg tcatctccga tatccctgcc cgttcacccg aatcggatgc
     4201 catgagcagg gatttgcaga aacggggatt taagttcgta ggttcaacaa tctgttacgc
     4261 gtttatgcag gctgcgggca tgatcaacga tcatctgaac ggttgcagca gaaatccact
     4321 gaaagcatga agtatgttga tgtcatagta cccctcccat tgtcgtcaac cttcacctat
     4381 gccgtacccg aagcgatgac tgacaccgta cagatcggga tgcgcgtggt tgtacctttc
     4441 ggaaagaaaa aaatgtatac tgccattgtc tgtatgattc acagtaacga gccaatcctc
     4501 tacgaagcta aagaaataat ttgcacactg gataatgagc ccatacttcg ttatccccag
     4561 ctaaagttct gggaatggat gagcaactat taccagtccg ccatcggtga tgtatatcag
     4621 gcagcagttc cggcaggcct caaacttgaa agtgaaacac aggtacgcgt caaccctgat
     4681 tacgagtccg aatcttcgct tactgaaaag gagtacaaat tgctggacgc cctctccgac
     4741 ggaaaaattc agagtgtttc tgacctcaac aaactgacag ggatgcgtga cacgcttcct
     4801 cttcttaaaa cactgatgga aaagggcgct gtagaagtca gcgaagaact aaccgaaaaa
     4861 ttcaagccaa aaaccgaaag ttatattaaa ctcagcgcct atgcttccga tgaaaataac
     4921 ctgcgggata tctttgacaa actaacccgg gcaaagaagc aactggaggt gctgatgacg
     4981 tacatcgagt tgtccaggtg tctttcaaaa acaaatatcc gggaagtttc cagaaaggaa
     5041 cttctggaaa aaaccaatgc tgcaacggct aatatcagcg gactaattga gcgcggagtg
     5101 tttgaagcct acagtaagga aaccggcaga ctggattatt cggatgtcaa cacagaagag
     5161 atgaatcctc tcaatgaatt tcagcaggaa gccatgaaca acatcaatag gcagtttact
     5221 gagaaacaag tcgtgctgct gcatggaata acatcaagcg gtaaaacaga gatatacatt
     5281 catctgatcg acaaagtgat agctaccggc aaacaagttc tctaccttgt accagagata
     5341 gcgctcacca cccagcttac aagcaggttg aaacgtattt tcggcaaacg cctcggtgtg
     5401 tatcattcga agttttcgga tgccgaacgg gtggaaatct ggaataatgt actgctaaac
     5461 aaaacctacg atgtcgttat cggtgtacgt tcgtcggttt ttctgccttt caggcaactg
     5521 ggactggtca ttgtggacga agagcacgaa aacagctaca agcagtacga cccggcaccc
     5581 cgctacaatg cacgtaatgc ggccattgta ctggcctcca tgcacggagc gaaaactctg
     5641 ctcggttcag cgacacccgc catagaatcc tactacaact cacaaaacgg taagtatggt
     5701 tacgtggctc tcaaccagcg ttatcaggag attgaactgc ccaacatcct gatagccgat
     5761 actaaagaag cttaccgtaa aaaggagatg gaaggtcacc ttaccccgct gctttcggat
     5821 aaaataacaa aagcactgaa caataatgaa cagataatac tctttcagaa caggcgcgga
     5881 tatgctccat tcctggaatg taagggatgc gcttatgtac cgaaatgcaa aaactgcgac
     5941 gtcagcctga cagtacacaa attcagcaac aagcttacct gtcattattg cggttatacc
     6001 gaaaacattc cttccgtttg cccggtatgt aaaacccccg gattggtgga taaaggtttc
     6061 ggaaccgaga aaatcgaaca ggaaattcag gaagctttcc cgggagcaaa ggtagcccgc
     6121 atggacctgg acacaacccg cagcaagaaa ggatacgaca aaatcatttc ggactttgaa
     6181 ctcgggaaag tggacattct ggtaggcaca cagatggtta ccaaaggact ggacttcgaa
     6241 cgggtcagcc tcgtaggcat actgaatgcc gacaacctgc tcaatttccc tgatttcagg
     6301 gcacacgaac gcgcctacca gctcatggca caggtgagtg gacgggcagg acgtaaaaat
     6361 aaacggggta cagtagtact ccagacttcc aatcccactc accctgttat tggccaggta
     6421 ctccggaatg attacaccgc catgttcaac acgcagtgtg atgaacgcag acaattcagt
     6481 tatcccccct acttccggct gatcgaaatc acccttcgcc accgcgacag ctcgacactc
     6541 aaccacgcag ccgaagagtt cggggtgaac atgaggaaga tattcggcac ccgtgtactt
     6601 gggcccacaa ttccgcccgt gtcacgaata cagaacatgt atattaaaca aattctactc
     6661 aaaatagaaa ccacagcatc agccgaaaag gcaaaacaaa tcctcggtga aattacctct
     6721 gccctgctga gccaaaatcc atacaaagcc gtccgcatca gcatcgatgt tgacccaatg
     6781 taagaatttc ctttccccac attacaattt atagcaaaaa tatccggata aatgaataat
     6841 tttgtatctt tgtgtcttca taaatacatt ttggaagcaa accggctgtt tttctaataa
     6901 ataacaaaca gacaccaata ataccttatt attgctccgg gttttatata aatagtaaaa
     6961 ttcaacaaat taagtattat aatactgaca aaaagtcaaa aaaccatgca agaaattaaa
     7021 ttctacagcg gagaaaatat tccgctggaa cttcacaaag tacgtatcgt acaaaaatta
     7081 aatctcgtac ctatcgaacg ccgtctgcaa gccctttttg aaggtggctt caacactttc
     7141 agacttagca ccagagatgt atttctcgac atgcttactg acagcggaac caatgccatg
     7201 agcgacaacc agctgtcagc catgatgcgc gccgatgacg cttatgctgg ttcacaaagt
     7261 tttgaacgcc tgcaaaaagc tgtggaagac gtattggata aaaaatacct gcttcccgtt
     7321 caccagggac gtgctgccga gaacatcctt tcagttgctt atgttcacaa aggaagtctg
     7381 gtacccatga attaccactt tactacagct atggctcata tcaccgacaa aggtggtaat
     7441 atagccgaat tgttgtatga tgaagcatac gtaatcaact ccgatcatcc tttcaaggga
     7501 aatatgaata ttgagaaact ggaggaaacc atcaactacc acggacccaa aaacatacca
     7561 tttatccgca tggaagcatc caccaatctg attggcggac aacctttctc ggtacaaaac
     7621 ctgagagatg tacgcgcgat tgccgataaa tataaaatcc gcttaatact tgactgtagt
     7681 ttattgggtg agaatgcctt cctggtattg cagcgtgagg aagagttcaa aaactccaac
     7741 atggaaacag tcatcaaaac catgaccggt ctggctgata tctgctattt ctctgcacgc
     7801 aaactcagtt cttcgagagg tggcggcatc tgtaccgaca gtctggagat tttcaaggaa
     7861 ctcgaagcat ttattccgct ctacgaagga tttcttactt acggaggaat gtcaatccgt
     7921 gaggtagaag caatggcagt aggcttatat gaaacactgg acgaaacagt aatcagccaa
     7981 agtcccagct ttatcaagta tctggtggaa caacttgaca aaaagaatat ccccgtaatc
     8041 aaacctgccg gtgtactggg tgttcacctg gatgctatgc agatttgcgc tcatatccct
     8101 caaacacaat atcctgcggg ctgtctggca gctgccctgt tccttatctc cggaattcgc
     8161 ggcatggaac gcggttccat ctccaaccag cgcgatgagt acggcaacga aacctatgct
     8221 gatatggagc tgttgcggtt ggctatgccg cgaagagtat tcaccctgtc tcaggtaaaa
     8281 tacgttgaag accgcgttac ctggctttac gataacagac atcttatcgg tggattgaaa
     8341 tttgtgtatg agccgccggt acttcgcttc ttcatgggtg gactggaacc tgtcaacgac
     8401 tggcctgaaa aacttatggc taaattcaag gaggacttcg gagaaagcct ttaagggtat
     8461 ccatatacaa gaatataaac gaagattttg ctttttgcaa aatcttcgtt ttttttatat
     8521 atgtttgttt atatttgtag atgacattta ataccaacgc gtatgataac acatttatta
     8581 aaccatcgta tatcgcccga tgcaatcacc gaattgcggc gaaatgaggt atttgtcttt
     8641 gggagtaacc tggatggcct tcattatggg ggcgcagccc gtatagcaca tgaaaaattc
     8701 ggagctgttt ggggacaggg cacaggcctt catggaaaat cgtatgccat accaaccatg
     8761 cacggaggag ttgatcaaat caggccttat gtggatgatt tcattgcttt tgcagcaagc
     8821 catcctcagc tcacctttct ggtaacacgc gtcgggtgtg gtattgccgg atttaccgac
     8881 aaggaaatag ctcctctgtt tgctcaggca actggctgca acaacatctt tcttcccgaa
     8941 agtttctgga atgtgctcag agaatgacta taaaacctac tgtccatctt ttcgggttat
     9001 ttcagtcatt actttcgttg ttctgatacc ggtatcgccg gtactcggac aaatgcccac
     9061 accccgcagc tgattgacta tagtctgaat caggggttgc tgaatatgtt ccggatgagg
     9121 aacttccact gtttctgtcc catcggcagt tgtaatttgt attttcatcg gactgccaaa
     9181 ggtattgaac accaggtgac cttttgatcc tgtaatggtg atggtctcct tttccgaagc
     9241 cttatcggtg gcgaaacacc aggttcccga gccgacaaca ccactttcaa acaacatgga
     9301 tgccgaaaca atatcatcgg caggatacat tccggcctgg ttagcagtaa caccaaaagc
     9361 tttaacaacc ggtccaaata cagaatcgag atagtccagc tgatgcgcgg ccagatcatg
     9421 aaaatgaccg ccaccggata tttcaggaaa aacgcgccag ttcccttcgg tatttatatc
     9481 gtacgaacgt ggagcgcgcc acatttctat ttcaacaaac cggatttcac ctattcctcc
     9541 tgctgtaata atctccctca acttattaaa tcccggcaat gcccgccggt agtaagccac
     9601 aaacaacgga acatccgctg ccttacatgc ttctatcatg ctgatacatt cctcatagga
     9661 atttgccatg ggtttttcca catagacagc tttaccggca gctgccgccc gaagtgtcag
     9721 ttcacagtga gaatcgggcg gtgtggaaat atagataata ttaatatcgg ggtcattcag
     9781 gatttcatcc acctcggtag tccattgact gatgccgtga cggtgcgcaa aatctgcagc
     9841 tttgtctgct gtccgacgca taatcatttt cacttcagaa tgctccgtct tgtacatacc
     9901 cggaacactt tttacttcac atacatttcc tgctcctatc accccccaaa taaccttttc
     9961 cagttgtttc attgttgtca gtctttaatt taccgatctg ttttgatgtg caagttaata
    10021 aattatgaat attccggaaa atatttgtcc tattatttgg aatctgtgaa atcatatcgt
    10081 agctttgtga tatcatttta atatcataat actaacacaa aatgaaaaca aaagaagaag
    10141 tcttttccgg atttaaactg gcaggtatcc ctgccctgtt actaaacttt attttccttg
    10201 tcggtattgt tttgctgttt atatacagcg taagctccct ttcacagagt ataattggcg
    10261 ttatactgat cattacctgt atcctgttgt tcatatgcaa catggtggcc tggggaggtt
    10321 tcatgcaaat tgaaccgaac gaagcccgtg tcatgatttt ctttggaaaa tacaacggaa
    10381 ctgtaaaaga aaacggattt ttctgggtaa atccattcta tgccaagaaa aagctgacac
    10441 tgcgcgcccg taacctggat gccgagccca tcaaggtgaa cgacaaagcc ggtaatccgg
    10501 taatgattgg catggtggtt gtatggcgcg tggtggatac ctacaaagcc tcatttgacg
    10561 tggataatgc atccatgact tcgggagcac ccggcacagc caccgataat gtcaatgcca
    10621 aaatgaaagc atacgagaac tttgtgaaaa ttcagagcga tgccgccctc cgaaaagtag
    10681 ccggcatgta tgcttacgat cacaacacag ccggtgatga tagcagcctg acattgcgcg
    10741 cgggcggtga agagatgaat gaaatactcg aaaatcagat taatgaacga ctgagaatag
    10801 ctggtatcga agtgatagaa gcacgcatca actatattgc ctatgctcct gaaattgccg
    10861 ctgtgatgct tcgtcgccag caagccgacg cgatcattgc cgcccgggaa aagatagtgg
    10921 aaggtgcggt aggtatggtg aaaatggcac tcgacaaact gtcgaaagac ggaattatag
    10981 aactggacga cgaaaagaaa gcagccatgg tcagcaacct gttggtggta ttgtgcggag
    11041 aatcaaacgc tcagcctgta atcaactccg gaacgcttta taacggataa taaacagatg
    11101 ccggcagata acaaacaaaa gaatttcgtt cttcgcattg acagtgaact gatggaagct
    11161 atcgaaaaat gggctgctga tgagtttcgc agcaccaacg ggcagatact gtacctgctt
    11221 gatcaggcgt tgagtaaaag cgggcgaaaa ccgaagaaga aaacatagcc ggactaaccg
    11281 gcttcaattt acccggcaaa ggtttcacag agtagtgaga cctttgtttt tatataaact
    11341 caatccttta atccaaaaaa gaatactaaa tttgcactcc gataaatgac agatatttta
    11401 tcttaaacta tcagaaaatt tcgacgacag cgcgggaaaa atgtcagcat gcagcaacac
    11461 gacggaatac gatacatgca tgtacgcagt taaagtggga aaaactcaca taaaatcgtg
    11521 gtgcaataaa aagatcataa aattgacaat agatgtcatc cgaagccatc atcttacttg
    11581 gaagcaacac caaccgtaac ctgctaacag aagcagcagc attgctcacg cgtgactttg
    11641 agattaaagc ctgttcgggt atagcggaga ccggagccgt gggatcgcat tacctcagcc
    11701 cgttctacaa tcaggcaata ttgctgcatt cccttcacaa ccgggatgaa gctgtcagga
    11761 tcttcaagga gaccgagaaa aaactcggaa gaacacctca aagcaagcat cagggcattg
    11821 tccccatgga tattgattta gtcgtgtgga acggagaagt ctgtcacggg gattacttcc
    11881 ggtacgactt tgtacgggct tgtgtgaatg aaatatgggc gaaagagtag ctgattcgtt
    11941 cacagtaact aaaaattcat tatatttgtg gttttctgca agtataatta atttcttttt
    12001 ttaaactcac atttgacatg aaacactttc caccatccga actgctgatc aatgatgacg
    12061 gcagcatatt tcacctccac ctgaaacccg aacagctggc cgataaaatc atactggtag
    12121 gcgaccccgg tcgggttgct cttgtagcct cctatttcga cgaacaggaa tgttctgtat
    12181 ccagtcgtga attcaacacc ataacaggca cttataaagg caagcggatt acagtagtat
    12241 ccaccggcat cggtaccgac aacattgaca tcgtggtgaa tgaacttgat gcgctggcaa
    12301 acatcgatct caataaccgc accgagaaaa ccgaattccg ccaactcaca ctcgtacgga
    12361 taggtacctc cggaggcatg cagcccgata taccactcgg cagttttctg atatccgaaa
    12421 aatcaatcgg
//
LOCUS       DAOPYB010000078        12351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5744_length_12351_cov_9.586939, whole genome shotgun sequence.
ACCESSION   DAOPYB010000078 DAOPYB010000000
VERSION     DAOPYB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5744_length_12351_cov_9.586939"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1283)
                     /locus_tag="PKH58_07425"
     CDS             complement(135..1283)
                     /locus_tag="PKH58_07425"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNX88897.1"
                     /translation="MNKTINNLNVFHVRDTTLLSRERVLSVDALRGFDMFWIIGGGDI
                     LTSLDKVFHNKITGFISEQLIHVDWFGFHFYDIVMPLFLFLVGISMVYSYRKRLSTEK
                     SDAPLWKHTIKRIIILWFLGMVVQGNLLSYNINEFHLYSNTLQAIASGYLIATIIILY
                     LPVLHQVTATIGLMLLYWMIMALIPVNGTTLGAYTPDGNVARIVDAFILGRFDDGLDY
                     TWIVSSLNFGATTMLGVFCGYILQSGLSKLKKFRNFLIFGAMLIILALILNIWHPIIK
                     KLWTSSFVLFSGGICVLLLSVFYLIVDVWNLQKSSKWLIIIGSNAIFAYVVSHLFGNQ
                     LISIAGVLLDGLKQYIGNWDEAVSITGGFLVLFFILRYMYKNKIFIKI"
     gene            complement(1321..3333)
                     /locus_tag="PKH58_07430"
     CDS             complement(1321..3333)
                     /locus_tag="PKH58_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88898.1"
                     /translation="MRHLLIISLFFLSFGVTAAQSTAGNSAGSVRIRVKNSSIEVENN
                     SLKAKWQVINRVVYAQNFVNKETKQKINWQSNSWFYLELENGERLTSKDFILTKNPVT
                     APLYSNAQAIKISDRFKGQSITADFYNEKTGLTLHWELSLKEESNYVKQKFTLSAKDS
                     VRVSKIGLIELPKEKGLIRNGVVAGSPLTGNGLFFAFEHPMSHTDTLKQSLGLYIKRY
                     KPVTSGNPLTFSAIWGMVQQQQLRRSFLYYLERERAVPYHHQLHYNSWYDISWEDRKL
                     DEASCLDRIQTFADSLIVKRKTSMDAFLFDDGWDNNQTLWQFNDGFPGGFSKLSERAK
                     KYNSDLGIWISPWGGYDQAKIQRLEYGQKQIPPFETNANGFTLSGNNYYKRFFDVASG
                     FVKNQGIEMFKFDGVGAGDGSAGANASYEKDIDALLKLIPDLRMIKPDLYFSLTVGTW
                     PSPYWLYYGDAIWRNGSDTGLSGDGSKRQQWMSFRDGEAYKNIVKRGPLYPLNSLMYH
                     GICIADQGVPGSLEMSDKDIADEIWSFFGTGTSLQELYINPHKLNSNNWDCLAKAINW
                     AKENENIQVDTHWVGGSPSDNQVYGFASWSPQKAILTLRNPSTKKQKFKIDVVEVFET
                     PNNKKIEYNFFDARTGNKVQPLASGSSFEIELQPLEVKVFDACPVK"
     gene            complement(3502..4893)
                     /locus_tag="PKH58_07435"
     CDS             complement(3502..4893)
                     /locus_tag="PKH58_07435"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNX88899.1"
                     /translation="MKNKKFNTILILFSLFLSLSCNNNNPVPDDGLLDVSYEESDSTI
                     LNPERGFYSQQEFNPSNMSKVLNVSTVKMQRTMGRTLILTMYYLNQFRDKPISNEFLS
                     IIGTNMKALREGGCKCILRFAYTSSESDKPWDAPQDIVLQHIQQIAPYLIEYSDVISV
                     VQAGFVGVWGEWYYTTNFIMSPYTQDEYAPRKAVLDALLLSLPKERMICVRTPVFKVK
                     CFNITYNDTISQATAFNQSDLSRIACHNDCFLADASDMGTFMNSQQMTFWEKESRYTA
                     MGGETCQLSGYSVCSNALKQMEKYHWSYLNSGYNGAVINDWKTNGCMNEINNRLGYRF
                     VLTQGKFTEKPVAGKEFISTLTVKNVGFASPYNPRDVKLVFISKTDMTDKYTVNLDCD
                     PRKWFAGGEYTIDVKYTLPQEMKGKEYVVYLNLPDPKETLANRPEFSIRLANKKVWSE
                     STGLNKLLTITVN"
     gene            complement(5213..6082)
                     /locus_tag="PKH58_07440"
     CDS             complement(5213..6082)
                     /locus_tag="PKH58_07440"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX88900.1"
                     /translation="MKKSIGLSFLAVLAILFFSCKSTVPPVVSFTYEMKQDTAVFTNT
                     TEGATSYSWNFGDNGTSTEQDPVHIYANSGTFNVTLTATNEGGSQTFTETVIIEKPII
                     KVDGSFSDWAEVTSDKLFTSTVADTATFKALQTLKVCSDNSYIYFYVKLDSAKVAPID
                     IFINSDNVATTGSNSWLWDNSAADYLIEGFLNVKMADATVFNFPATQADQTAWAWAES
                     TPTGSGVVTLSKPKTVSGTVVEIEGKIIRELVTPNWASTIGFGIFTSGTDWSITGCLP
                     GGGNKGKLLQVKL"
     gene            complement(6146..7819)
                     /locus_tag="PKH58_07445"
     CDS             complement(6146..7819)
                     /locus_tag="PKH58_07445"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX88901.1"
                     /translation="MKKLNLLYVIPMLFILTSCNLDFAPTDGMTPEQLAKDPSGAAYM
                     TDGNYSLFKDEFATSWTYQSGNSYIRHYSMMSEYPSDNICLSGRTTDPLYEATCYKTN
                     STLANVTTLWWLGYKIIYGANSVIESVKDGTDKQSDYIKGENYFLRAICHLHLVTMYA
                     KPYTSGRSNPGIVLRNSTDNSKTERATVGEVYDQIVADLKEAIRLMGNGGTRRGNAGY
                     ASKAAAQGLLSRVYLYMEENQKVIDLVNEMLDGADPSTKLEPSATFPKYFANALTSKE
                     TLWAVAHTDLETRGQASIGSMYLNDGTGWGETYSSDPLNNLYERYPNDLRYSYVVPVY
                     KTNATNYMITFPVTSEGVDFRSNEIHDVTADANGKYYFTNNSAKVYVESETVNGYKQN
                     YINFNGNKYSVRLTKKMADRNTFPKYYITKFSYQNNNPMLSSPVMLRWAEVILNRAEA
                     YAKLNQNDKALNDVNVIRTRAGLSGDALFSATNMHGYTSVLDVVLDERRLELAFEGHR
                     MIDVYRNKRSMNRQYAGVQPWEIINYDNNKIQYPIPSNEILVSGIEQNK"
     gene            complement(7853..11119)
                     /locus_tag="PKH58_07450"
     CDS             complement(7853..11119)
                     /locus_tag="PKH58_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX88902.1"
                     /translation="MDWLKIGVLYVLLALTGTLNAQNYTQPVSLNLKKSTFKNFIKQI
                     EAKTQFSIIYRDALIDDKKDITITVSNRPLNEVMKTVLSPKGLQANYKNKTIVITKAK
                     AEIKQETGTQDAKKRRITGKVFDSQTGETIIGASVIVKGENGGTTTNIDGEFSVEANP
                     QSVLQISYVGYKGKDIVLGDASDLNISIEEDVRKLDEIIVMGYSSQKKSELSSSAVTL
                     SAEKLTDVATSDIGNMLQGKVAGLVVYNSSGQPGSAAQISIRGTGSITADADPLYVVD
                     GIPGGSFNPNDVQTLTVLKDAGATALYGSAAAGGVIVVTTKSATRNQPTKVDVKANIG
                     SKSNLFGHFKMMDSQELYYMQKKLFSPALFSLMRPSGLLHQDYNWQDAFFKTGTQQDY
                     YVSASGSNNKTTYFASMDYYNEEGTLINTGLKKLSGRLNLNSQLYKNLDMNIRLSYTN
                     SDIQSPSSWTTLNDAYTKMPWDSPYDANGAIEKITSATRADGSVWYSQDKWNSLHNEQ
                     YNYNKSHSFDLVGDFQLNWTIKDWLIFTTSNRYNQNAYKTVLFIDPRTYSSSYSDGYL
                     LNEISFGKSFGTTNTLKANNKFGNHSLNSLLGWEWGQSTTELTTASGKGMPNGMDALN
                     ACEIYEIGGYSIPLRSYSGFGQVQYSYLNKYYATASLRGDANSKFAEKRRLGWFPSGA
                     ASWLISNEDFLKGNKTITFMKLRGSYGLTGNSNIGMYQYLSSYTLSSSYQNVVSAIPT
                     RLSNMYLGWEKAYMAGIGLDINLWKSVELNIDLYNTDNKDLLLAVPKSPSTGFFEVTA
                     NAGSVRNQGIELQLNTININKKNFRWDMGFNFGLNRNVVTETPNDAAFLQTRSSVSQQ
                     IKRGQDIYSWYMPKWLGVNPENGDPLWEKVVYDAAGNITDRQQTNNYNETTFQVVGKA
                     TPKFSGGWSNNLTYKGFFLSLNTNFVYGNKIFNYNRLSLDADGAYLGYNQISLENNGL
                     GWTRWEKAGDIATHPKLVMNGNKSSNNISSRYLEDGSFFRIKNLKIGYEVPAKLLRVA
                     HIQNCKFYVSGDNLFTLTRFSGMDPEVSIRTSAYSLAGLYSDNYPVSRQILVGVEFGF
                     "
     gene            complement(11251..12225)
                     /locus_tag="PKH58_07455"
     CDS             complement(11251..12225)
                     /locus_tag="PKH58_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNX88903.1"
                     /translation="MENFITKLYKKYLYDHITYDEFVRMRHAMNDINDEGLSELMEDE
                     WDENIFQEKLRKESKDNIRKSLGFYIENENKWQSKKRIIGVAAVLIPILIVSTFFLTK
                     SLSVHEPGNFIVSVERGNKALVTLPDSSKVWINSNSTLEYNKGAKNTRQVRLTGEAFF
                     KVYKDKSRPFIVTMNNLHVEVLGTSFNVKARQGADIIETSLVEGSVKLRSSDLSQDYY
                     LEPNEKAIFSQSTRQMQIIHTDNDLETAWKDNKLKFSSERFIDVMSMLEEWYGVKIIC
                     KCPEIENDLISGSFNDESLESTLEILKMQYKINYLRNRDTITIVNRIR"
BASE COUNT         3442 a         2536 c         2322 g         4051 t
ORIGIN      
        1 gaagcggatc attttgctgc ccttatttca caataaaaaa ataagaaacg gttagagttt
       61 acccctaacc gtttcttatt tttatcagtg ccggttgaaa tacattcata ttccgctgta
      121 gtgataaatg gctgttagat cttaatgaat atcttgtttt tgtacatata acgcagaata
      181 aagaacaaca ccagaaaacc tccggtaatg cttactgctt cgtcccagtt accgatatat
      241 tgttttaatc cgtctaacaa aactccggct attgatatta actggttgcc aaacaaatgc
      301 gacaccacat aagcgaatat cgcgtttgaa ccaataataa tcaaccattt cgaacttttc
      361 tgaaggttcc aaacatcaac tatcagataa aatacactaa gaagcaaaac gcatattcct
      421 cctgagaata gaacaaatga gctggtccat aatttcttta taatcggatg ccatatattt
      481 agtatcagcg caagaataat aagcatcgct ccgaatataa ggaaattcct gaattttttc
      541 agcttgctta atcctgactg caaaatataa ccacagaaaa ctcccagcat ggtggttgct
      601 ccaaaattca gacttgaaac aatccatgta taatccagtc catcgtcaaa tcttcccagt
      661 ataaaggcat ctacaatccg ggctacattt ccatcgggtg tatacgcgcc cagtgttgtc
      721 ccgtttactg gaattaaagc catgatcatc cagtataata gcattagtcc aatggtagcg
      781 gttacctgat gcaataccgg taaatagagt ataattattg tcgcgataag ataaccggat
      841 gcgatagcct gaagtgtgtt tgaatacaaa tgaaactcat tgatattgta actgagtaag
      901 tttccctgta caaccatacc caggaaccac agtatgataa tcctttttat ggtgtgtttc
      961 cacaaaggag catccgattt ttcggtactc aaccgtttac gataggaata caccatcgag
     1021 atgccgacca aaaaaaggaa gagaggcata acaatgtcgt agaaatgaaa gccgaaccaa
     1081 tctacatgta ttaattgttc ggaaatgaaa ccggtaattt tattgtggaa aactttgtca
     1141 agtgaagtga gtatatcacc tcctccaatt atccagaaca tatcaaagcc ccttaaggca
     1201 tctaccgata aaactctttc tcttgatagc agagtagtat ctctgacatg aaatacgttt
     1261 aagttattaa ttgttttgtt catttaattt gatatttgtt gtatgaattg atattgatat
     1321 ttattttacc ggacaggcat cgaatacttt tacctctaat ggttgtaact cgatctcaaa
     1381 actacttcca ctggctaagg gttgcacttt gtttccggtt cttgcatcaa agaagttata
     1441 ttcgatcttt ttattgttcg gtgtttcaaa tacctcaacc acatcgatct tgaatttttg
     1501 cttttttgtc gatggatttc ttagtgtcag tatcgctttc tgaggtgacc aggaggcaaa
     1561 tccataaacc tgattatctg agggtgaacc accaacccaa tgggtgtcaa cctgaatgtt
     1621 ttcattctct tttgcccagt taatggcttt tgccagacaa tcccagttat tagagtttaa
     1681 cttatgcgga ttgatatata attcctgcaa actggttcct gtcccgaaaa acgaccagat
     1741 ttcgtccgcg atatctttat cagacatttc aagggaaccc ggaactcctt ggtcggcaat
     1801 gcagatgccg tgatacatca atgaatttaa gggatataaa ggacctcttt ttacgatgtt
     1861 cttataggcc tctccatccc ggaagctcat ccactgctgt cttttcgacc cgtctccgct
     1921 taaacctgta tcgctgccat tgcgccagat ggcatcgcca taatataacc agtaaggcga
     1981 tggccatgta cctacagtca ggctgaaata taagtcgggc ttaatcatcc gtaaatcagg
     2041 gattagcttc agcaaggcgt caatatcttt ctcgtaactg gcgtttgctc cggcagaacc
     2101 atcacctgcg cctacgccat caaacttaaa catctcaata ccctgatttt ttacaaaccc
     2161 ggacgctaca tcaaaaaagc gtttatagta attatttccg cttaaggtaa agccgtttgc
     2221 attggtttca aaaggcggaa tttgtttctg tccgtattca aggcgttgaa tttttgcctg
     2281 gtcataacca ccccagggcg aaatccagat acccaaatcg gaattgtatt tttttgcgcg
     2341 ttctgacaat ttgctgaaac cacccgggaa cccatcgttg aattgccaaa gcgtttgatt
     2401 attatcccag ccatcatcaa aaagaaaagc gtccattgat gtctttcgtt ttactatcag
     2461 gctgtcagca aatgtttgta tccggtcaag gcacgatgcc tcatctaatt ttctgtcctc
     2521 ccacgaaata tcgtaccatg aattataatg cagctgatga tgatagggta ctgcacgctc
     2581 tctttccagg taatacagga aacttcttct tagttgttgc tgttgaacca tcccccaaat
     2641 cgctgaaaac gtcaggggat ttcctgaagt gacgggttta taacgcttaa tatataaacc
     2701 cagcgattgt ttcagggtat ctgtatggct catgggatgc tcaaaagcaa aaaataaacc
     2761 attaccggta agtggcgaac cggctacaac tccgtttctg atcaatcctt tttcttttgg
     2821 tagttcaatt aatccgattt tactcacacg aaccgaatct tttgcgctta aagtaaattt
     2881 ctgcttaaca taatttgatt cttccttcaa actaagttcc cagtgaagtg tcagccctgt
     2941 tttttcgtta tagaaatccg cagtgatact ttgtccttta aaacggtctg atatcttaat
     3001 tgcctgagcg ttggaataga gtggggcagt aaccggattt tttgtcagaa taaaatcttt
     3061 tgaagttaat cgttcgccat tctccagctc cagataaaac caggaattac tctgccagtt
     3121 aattttttgt tttgtctctt tgtttacaaa gttttgtgca taaaccaccc ggtttattac
     3181 ctgccacttt gcctttaagc tgttattttc cacttcaata gaggagtttt ttacccgtat
     3241 tcttaccgaa ccggcagaat ttcctgctgt tgactgagct gcagttacgc caaatgaaag
     3301 gaaaaacagg cttatgataa gtaaatgtct catttttaat ttatagatag ggttaacgaa
     3361 ttagtgttca gattgaaacc tgttcataaa cacgatttca atactgcata tttatgataa
     3421 aatgaccccg gtacgggacg tattgaattg agtcaattct tcccgtaaga ttgaattatc
     3481 agaatgtaag gttgtattgt tttagtttac ggtaattgtc aacaatttgt ttagtccggt
     3541 tgattcactc cagactttct tgtttgccag acgaatactg aattcggggc ggttggcaag
     3601 tgtttccttt ggatctggca gattcaaata gacaacatac tccttgcctt tcatttcctg
     3661 aggcagtgta tatttcacat ctatcgtata ttcgcctcct gcaaaccatt ttcgtggatc
     3721 acaatccaga ttaacggtgt atttgtcagt catgtccgtt tttgaaataa ataccaattt
     3781 tacatcccgg ggattatagg gtgatgcaaa tccaacattt ttaacggtta gtgttgatat
     3841 aaattctttt ccggcaactg gcttctcggt aaatttgccc tgcgtcaata cgaaacgata
     3901 tccaagtctg ttattgatct cattcataca cccgtttgtc ttccagtcgt taatgacagc
     3961 tccgttataa cccgagttca gataggacca atggtatttt tccatttgtt tcagcgcgtt
     4021 actgcatacc gagtaacctg acaactgaca ggtttcacca cccattgccg tatatcttga
     4081 ctctttttcc cagaaagtca tttgctgcga attcataaat gtacccatgt cgctggcatc
     4141 agccagaaaa caatcgttat gacaggcaat acgcgataaa tcgctttgat taaatgcggt
     4201 agcctgagaa atggtatcgt tgtaagtgat gttaaagcat tttaccttaa ataccggagt
     4261 tcgtacgcat atcatccttt ctttcggtag tgacaaaagc aacgcgtcca gaactgcttt
     4321 acggggtgca tattcatcct gcgtgtaagg cgacatgatg aaattggtag tataatacca
     4381 ttcaccccaa actccaacaa atccggcctg cacaacagaa ataacatctg aatattcaat
     4441 caaataagga gctatctgct gaatgtgttg taaaacaatg tcttgaggtg catcccaggg
     4501 cttatcactt tcgctcgagg tatacgcaaa gcgtaaaata catttgcaac caccttcgcg
     4561 taaagctttc atgttggttc ctattatgga taaaaattca ttagaaattg gtttgtcccg
     4621 aaactgattc aaataataca tggtaagtat cagcgttctg cccattgttc tttgcatttt
     4681 taccgttgaa acgttcaata ctttactcat attggacgga ttaaactcct gctgactata
     4741 aaatccacgt tcaggattaa gaattgtaga atcagactcc tcgtaactta catcaagtaa
     4801 accgtcatcc ggcacaggat tattgttatt gcaggataat gacaggaata aagaaaacag
     4861 gatgaggatt gtattaaatt ttttgttttt catatgtaaa ttagcttatt aaatatagat
     4921 ttaaaggtcc gaacgtttgg aattgaaaat aattatcgac agagaaggaa gtattggctg
     4981 tcatggttgt tacctcgttt gacaggttta ttttatctga aataaaattc agatgctgag
     5041 gggattatat gtatagaaaa cactttcatg aacgattgat tcgtttttga tatgtaaccg
     5101 ttttatttgg cagaagaatt ggcgtttttt cttgaaaaat acaggcatgg attacgataa
     5161 aactgaaccc atgcctgcat ctccataaat caatattcca aaaatccaac tcttaaagtt
     5221 ttacctgtaa taatttgcct ttatttccgc caccgggtaa acaaccggta attgaccagt
     5281 cagttccgga agtaaagata ccaaatccga tagtacttgc ccaatttgga gtaaccagtt
     5341 ccctgataat tttaccttct atttcaacaa cagttccact gacagtttta ggtttagaaa
     5401 gagtgacaac accacttcct gttggagttg attctgccca tgcccatgct gtttgatctg
     5461 cttgagtagc agggaagtta aatactgtgg catctgccat ttttacattc aggaaacctt
     5521 cgatcagata atcagctgcg gagttgtccc ataaccagct attgctgcct gttgttgcaa
     5581 cattatcaga attaataaag atatcaatag gagcaacctt tgcactgtcc agttttacat
     5641 aaaaataaat ataactgtta tcgctacata cttttaatgt ttgtaaagcc ttgaaagttg
     5701 cggtatcggc tacggttgat gtgaaaagtt tatcactggt cacttcagcc caatcgctga
     5761 aactaccgtc aaccttgatg ataggttttt caataatgac tgtttctgta aaagtctgcg
     5821 agccgccttc atttgttgca gtcagtgtga cattgaatgt acctgaattt gcataaatat
     5881 gtaccgggtc ttgttcggtc gaagttccgt tatctccaaa attccagcta taacttgttg
     5941 ctccctcggt agtgtttgtg aatacagcag tatcctgttt catctcataa gtaaacgaca
     6001 ctacgggtgg aaccgttgat ttacaactaa agaacagaat agccaataca gctaagaatg
     6061 ataatccaat gctttttttc atgatgtttt agtaataaaa ttagtaatta agttaatttg
     6121 attttttgtt attttttcgt ttctgttatt tgttttgttc gataccgctt accagaattt
     6181 cattcgaagg tatgggatat tgtatcttgt tgttatcata attgataatc tcccatggtt
     6241 gaactccggc gtactgacgg ttcatcgaac gtttatttct gtaaacatca atcatacggt
     6301 gaccttcaaa agccagttcc aggcgacgtt catccaatac aacatcaaga acagatgtat
     6361 aaccatgcat gttggttgcc gaaaacagag cgtctcccga tagacctgca cgcgtccgga
     6421 ttacatttac atcgttcagc gctttatcat tctggtttaa cttggcataa gcttcagctc
     6481 tgttgagaat tacttcagcc cagcgcaaca tcaccggaga acttaacata ggattgttgt
     6541 tctgataaga gaatttagtg atataatatt tggggaaggt attacgatct gccattttct
     6601 tcgtcaggcg gacactatat ttgtttccat tgaaattgat ataattttgt ttgtaaccat
     6661 ttacagtctc actttccaca taaactttag ctgagttatt agtaaaataa tactttccat
     6721 tagcatccgc ggttacgtca tgtatctcgt tcgaacgaaa atccactcct tcgctggtta
     6781 caggaaatgt gatcatataa ttggtagcat tggttttata cacaggaact acataactat
     6841 atcgcaaatc attcggatag cgttcgtaca gattgttcaa tggatcggac gaataagttt
     6901 ctccccagcc cgtaccatca ttcagataca tggatccgat ggatgcctga cctctggttt
     6961 ccagatcggt atgtgctact gcccataaag tttcttttga agtaagtgca ttggcaaaat
     7021 atttagggaa agtagccgat ggttccagtt tggttgatgg atcagctcca tccagcattt
     7081 catttacaag atcaatcact ttttgatttt cttccatgta caggtaaacg cgtgaaagca
     7141 atccctgagc ggcagctttc gatgcatatc cggcatttcc gcgtcttgtt ccaccattac
     7201 ccattaatct gatcgcttct tttaagtctg caacaatctg atcataaacc tcaccaacag
     7261 tggcacgttc tgttttagaa ttgtcggtgc tgttacgcaa tacaataccc ggatttgaac
     7321 ggccagaggt atatggtttg gcatacatcg taactaaatg taaatggcaa atcgcgcgta
     7381 aaaagtaatt ttcaccttta atgtaatccg attgtttatc tgttccgtct ttcaccgatt
     7441 cgatgactga gtttgctccg tatataatct tgtagccaag ccaccacaat gtagttacat
     7501 ttgccagggt cgaatttgtt ttgtaacagg ttgcttcgta taagggatcg gtagtacggc
     7561 ctgacagaca gatgttgtca ctgggatatt ccgacatcat tgagtaatga cgaatataag
     7621 agttaccgct ttgataggtc cagctggtgg caaattcatc tttaaataag gaatagttgc
     7681 catctgtcat ataagcggct ccgcttggat cttttgctaa ttgttcggga gtcattccat
     7741 ctgttggggc aaagtccaga ttacaggatg tcaatatgaa taacatcgga atgacatata
     7801 ataaattaag ttttttcatt tttgtgttta tatttcgttc gcgttattgt tattaaaaac
     7861 caaattctac tcctaccagt atttgacgac taacaggata attgtcacta taaagtccgg
     7921 ctaaggagta tgctgaagtt ctgatggata cttcggggtc cattccggag aatctggtta
     7981 atgtgaacag attgtcacct gaaacgtaga atttgcagtt ttggatatgg gctactctca
     8041 atagttttgc aggtacttca taacctattt tcaggttttt tatccggaag aaacttccgt
     8101 cttccagata tcgggaagat atgttgtttg atgatttgtt gccattcatc actaattttg
     8161 gatgagtagc tatatcacct gccttttccc aacgggtcca gcccaaacca ttgttttcga
     8221 gcgatatctg attgtaacct aaatatgcac catcagcatc gagtgataaa cgattgtagt
     8281 tgaatatctt gtttccatac acgaagtttg tgttaagact caggaaaaat cctttatagg
     8341 tcaggttgtt ggaccagccg ccgctaaatt tgggagtagc ttttcccacc acctggaaag
     8401 ttgtttcgtt ataattgttg gtttgttggc gatcggtaat gttaccggct gcgtcataaa
     8461 ctactttttc ccagagtggg tcaccattct caggattgac acccagccat ttaggcatat
     8521 accagctgta tatatcctgc ccgcgtttta tttgctgact gacagagctt ctggtttgca
     8581 gaaaagctgc atcgtttgga gtttcggtaa caacgtttct gttgagtcca aagttgaaac
     8641 ccatatccca tctgaagttc ttcttgttta tgttgatagt gttcagttgt aattcaatac
     8701 cctgattccg tacgctgccc gcattggcgg ttacttcaaa gaacccggtt gaaggcgatt
     8761 ttggaacagc aagtaataga tccttattgt ccgtgttata caggtcaatg ttcagttcaa
     8821 cgcttttcca taagttgata tccagaccaa taccggccat gtaggctttt tcccatccga
     8881 gatacatatt tgataatcgg gtagggatag ctgaaacgac attctgataa gaactgctta
     8941 atgtatatga cgaaaggtat tgatacatcc cgatgttgga attaccggta agtccgtaac
     9001 ttccgcgcaa tttcataaat gttatagttt tatttccttt cagaaagtcc tcgttgctga
     9061 ttaaccagga agctgcgccc gaaggaaacc agccgagacg tctcttttcc gcgaattttg
     9121 aatttgcatc tccacgcagg gatgctgtag cataatattt attcagataa ctgtactgaa
     9181 cctgtccaaa ccccgaataa cttctcaggg gtatggaata tcctccgatt tcgtaaatct
     9241 cacaggcatt taatgcgtcc attccgtttg gcatgccttt tcccgaagct gtggtcaatt
     9301 cggtcgtaga ttggccccat tcccatccaa gcaggctgtt taatgagtga ttgccaaatt
     9361 tattgttcgc tttcagggtg tttgttgtac cgaaagattt tccaaaacta atttcgttaa
     9421 gcagataacc gtcggaatag gatgaactat aagttcttgg atcaataaac agtacggttt
     9481 tgtacgcgtt ctggttataa cggttggagg ttgtaaatat taaccagtcc ttaattgtcc
     9541 agtttaactg aaaatctccg acaagatcaa aagaatgtga tttgttataa ttatactgtt
     9601 cgttgtgcag tgaattccat ttatcctgtg agtaccacac cgacccgtca gcacgtgttg
     9661 cgctggtgat tttttcaata gctccgtttg cgtcataggg agaatcccag ggcatttttg
     9721 tatacgcgtc attcagtgtt gtccagctcg aagggctttg aatgtctgaa ttggtgtagg
     9781 acaaacggat gttcatgtct aaattcttgt acaactggct attcaggttc aaacgtcctg
     9841 aaagtttctt cagaccggta ttgatcaacg taccttcttc gttatagtaa tccatgcttg
     9901 caaagtaggt ggtcttattg ttgcttcccg atgccgaaac atagtaatcc tgctgtgtcc
     9961 cggttttgaa aaatgcatcc tgccagttgt aatcctgatg caacaggccg gatggacgca
    10021 ttaatgaaaa taatgccgga ctaaaaagct ttttttgcat gtaatacaat tcctgcgaat
    10081 ccatcatttt gaagtgtcca aacaaattcg atttcgaacc tatatttgct tttacgtcca
    10141 cttttgtagg ttgatttctg gtcgcggatt tggtggtaac aacaataacg ccgccggccg
    10201 cagctgatcc atacaaagcg gtagcgccag cgtctttcaa aacggttaaa gtctgtacat
    10261 cattgggatt gaagctgcct ccgggaatac catcaacaac ataaagcggg tcggcatcgg
    10321 ctgtgatgga accggtacca cgaatgctga tctgggctgc agaaccaggt tgaccgctcg
    10381 aattgtatac gacaagacct gccactttac cctgaagcat atttcctatg tcggacgtgg
    10441 caacatcagt cagcttttcg gctgaaagcg ttactgcaga actggataat tcggattttt
    10501 tctggctgga atagcccata acgatgattt catccagttt cctgacatct tcttcgatgg
    10561 aaatattcag gtcactggca tcgcctaaaa cgatatcttt tcctttataa ccaacataag
    10621 agatttgtaa aacggattga ggatttgcct ctacagaaaa ttcaccgtca atgttagtag
    10681 ttgtacctcc attttctccc ttcacaatta ccgatgcacc tataatggtt tcacctgtct
    10741 gcgaatcaaa aactttaccg gttattctcc ttttctttgc atcctgtgtt cctgtttcct
    10801 gtttaatctc tgcttttgct tttgtaatta caatcgtttt gttcttataa ttcgcctgta
    10861 gtcctttcgg tgaaagcact gttttcatta cctcgttcag cgggcgattg gaaaccgtga
    10921 tggtgatgtc tttcttatca tcaatcagtg catcccgata aataattgaa aattgagttt
    10981 tggcttcaat ctgtttaatg aagttcttaa atgttgattt ttttagattt aatgaaactg
    11041 gttgtgtgta gttttgtgca ttcaatgttc ctgtcaatgc taacaaaacg taaagcacac
    11101 ctattttcaa ccaatccatt cttctgagta acaaaagttt tttctgatgt gtgtacatgg
    11161 tgtttttttt tgataagata tttataaatg aatatatatg tttgtttatt cctttttgtc
    11221 atcatgtaaa caccatcaac gaaaccgtct tcatctgatc ctatttacaa ttgttattgt
    11281 atctctgttc cttaagtaat taatcttata ctgcattttc aatatttcca gcgtactttc
    11341 gagactttca tcattgaaac ttccgcttat caggtcattc tctatttccg gacacttaca
    11401 gattattttc acaccatacc attcttcgag catcgacatt acatcaataa atctttcaga
    11461 gctgaatttc aacttgttat ctttccaggc tgtttcaagg tcattatcgg tatggattat
    11521 ttgcatttgt ctggtcgatt gactaaaaat cgctttttca ttcggttcta aataataatc
    11581 ctgcgacaga tcagaacttc gaagttttac acttccttcc acaagcgagg tttcaatgat
    11641 atcggctccc tgccttgcct taacattaaa tgaagtaccc agtacctcta catgtagatt
    11701 attcattgta actataaatg gccggctttt atctttatat accttgaaaa atgcttcacc
    11761 ggttaatcta acctggcgtg tgttttttgc acccttatta tattcaagtg tactgttcga
    11821 gtttatccac actttcgagc tgtcgggtaa cgtgaccagc gctttgttgc cacgctcaac
    11881 cgatacaatg aaattgcccg gttcatgcac ggatagcgac tttgttaaaa aaaatgtact
    11941 gacgattaaa attggtatca gaacagcagc taccccgatt atcctttttt tagactgcca
    12001 tttattttcg ttttctatgt agaagccaag cgatttccgt atgttatcct tcgactcttt
    12061 cctgagtttt tcctgaaaga tattttcatc ccattcatct tccataagtt cagagagccc
    12121 ttcatcattg atatcgttca tcgcgtgacg cattcttaca aactcatcat acgtgatgtg
    12181 atcgtagaga tatttcttat ataatttggt tataaaattt tccatttttt tctttcttta
    12241 tattacacgt tagacatcaa aaaagatact atgcgatttt ttatgttata aaacatattt
    12301 cgaaaataat caggaataag atacttttct tcgacaattg tgaacgtatc t
//
LOCUS       DAOPYB010000079        12299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5779_length_12299_cov_10.643417, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000079 DAOPYB010000000
VERSION     DAOPYB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5779_length_12299_cov_10.643417"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKH58_07460"
     CDS             <1..213
                     /locus_tag="PKH58_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88904.1"
                     /translation="TFTPSYAVSDAAVRGSYAVLIFGVYSVNVMGITLTGILVWLINF
                     VIPMLAGSFFVLKAKSWKSVESPSQE"
     gene            complement(227..1009)
                     /locus_tag="PKH58_07465"
     CDS             complement(227..1009)
                     /locus_tag="PKH58_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HNX88905.1"
                     /translation="MSVNAGKQTKIVILHTNDTHSQVEPAESNNMGGYARRMGEINKI
                     RSEEPNVLLVDAGDFSQGSPYFNFFNGRIETKALEMMGYDALTLGNHEFDNGLDTLAS
                     VLKLVKLPIVNANYDLKNTPIAPFVKPFIIVRKGGLKIGILGIGVNLRGLAYQKNFAG
                     LILNDPIRTAAETSDYLKHNLKCDLVICLSHLGTSYEDSSPTDYDLVAASRSIDVVVG
                     GHSHKILENVTAENVSGRKILITQMGKSGLNLGRIDLTFEKK"
     gene            complement(1069..2226)
                     /locus_tag="PKH58_07470"
     CDS             complement(1069..2226)
                     /locus_tag="PKH58_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX88906.1"
                     /translation="MTQTLFVISPLYVTLFWAIVLNTDVDKNTSPRTFLGKFMIFAFI
                     VYFSHFLYFDSLKVLYAWTDAIYQFASLMVFPMYYIYFRLLTIDEKFSLRKHARYLLP
                     SIVLFLLYLFGIMLTPVADYQSWLFQKDIPSASFGIAWLNVIFVLMKAVFVIQVVLVL
                     VANTILIRKYAYKAAQYYSDVHDSGTISVQLLNASMLFTALTSIVVAVLGRDYFVAHN
                     LLLGIPSLIFSVLLFTIGWLGNRQKPVNPAYEEFPFQTEALVELPRNSRREKSILKKI
                     MVLFEQEKIYLKSCVTIQDVANSIGTNRTYISSVINEHCQQNFCTFVNQYRLNEIEEI
                     LKSDNDITLNQLAEQTGFGSVDSMKRAVKLKTNMNFREWKEEIIKNTTNDK"
     gene            2562..7988
                     /locus_tag="PKH58_07475"
     CDS             2562..7988
                     /locus_tag="PKH58_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX88907.1"
                     /translation="MIRKTYQQQTNKQAMNTLQYNFRRSQTIFGFFVLLFVCISGCFN
                     QLDGQIKSTEAAGRFALNYFNSTAYKTKMQKVPASSADIELKYQSGGNVQAPIYIFQN
                     EQRGFVVFAQSTHGFQILGYSDKANFNPAEIPDGLQQLLKLYENASPDSIMGIQNTRE
                     TTTVVSPLLDQAGVGLNQFHHENVGGSWSGCMATAMTQIMCYYKYPDKGIGSHCYTNA
                     AYGELCADFGNTHYNWINPSDEDFKLLSYHVGVSMDMNYNGSPAGSSPSSPTSLYALH
                     DYFGYTCEPFNDFSRNSQVIQNVIAQQKPVYAHLLGNPGHAVVLDGYDSNGFFHINFG
                     WGGGGNGYYMLNNNSLIYSGNYKFGTNLAEVILYQPGPVHLDKTDSLALVSIKNNISN
                     LDWDLSDAQKRTGITTLNGRVMSLNIYSANMIGNEGSIPDDIDNLHELINLNISGKLH
                     GTIPQNISNLTNLQSLRISNFSGTLSDTIPTNIGNLTNLTNLSIFNAAKGPIPSSVGK
                     LVNLRSLNLPTGNMDGTIPEEIYSLNNLQEITLDDQKLAGNISESLGNLKNLIFLSIA
                     NNQLSGKIPSTIGNLNKLQYLDLSGNKLSGEIPPSLNNCTSLSTLKLFSNELEGKVPA
                     IFDNKFKLTSLDLSNNKLTEISENIGKLTNLQTLNLNNNQLSSLPDSLNELRVMNSFS
                     ASNNKINRLPANLNQLTSLNTLNLVNNGITVFPNDLCFLPNLSGIDLSYNKITRLPAM
                     ISDLKAQILQIQENELSGAIPANLLRTEPGYYRFQNNRFVYNDLPQGTDFTNLIGTQK
                     PLKLLKNTFKGNLGDSIEIDIRKICSNLNKHDTYVWCEYPKEKKEIQPTIKIEDGPVL
                     HLKLSDKNIGKKYYCLITNDSAATYKDPQIYYRIPCLSNLITDTLFLAIQTEDEFLSE
                     KYPEQYIVKSESMQKKELSDKTVTLISPFKMRGIKKWEASSNQKEWYELSASMTQNDL
                     KANLVSVNEEELKISPKTPAYYRCALLETNCAPHYSDTLKINPYGKVICDTTLNVQNK
                     TITFKKDSIEVTIPQGINDSDFRLTIVKLDNPPAKPDSVFRLSSVYDVTVSFGDSFYL
                     PVIIKLKNLNKRSFNQMNIDNYKAVYFNDIEQQWEYYNDANINLNDTTLTFGTYHFTK
                     LGYFEFNDWNYTHRLTRGRVNVIYNVSDQSIFDLYDGMVVKEGLKSWHDTNTNPDKDG
                     NPYLIQDIAEYMNQIINKCDLLGLETPGLRFNVYVKQVGNYAQIGAASYLSGRGFLYI
                     DPIFMSMEKTYKENTVQLMTTLAHEYSHYTQDYYMSMIINNYFWQEATAPLGGRMIWN
                     TTDLPLTEPEMLIKQGLSPTKGNKTIFEVLSASWFNDYNLPVVSKLASNSADYNLASL
                     FLHYMQNYRRGTTLDAAVLLKETSYLDTWLGYLESYIKKYLKSNTGTEFADYVKYIFS
                     GVNPNFTVINREDGNPYKYIIQNIDNKGDFANLLNYNFDKHDYQTNKISIKIPYLGAR
                     MYLLNNMTLDKAVVVKYKRLTEKNENEKTFYGKYDEKTKQMKIVEITDSTEYVFLLDA
                     LTPKSNTDFLNSGFLLFVNAKTNSTIFTDYNASFELSAMPVPNITALCYADVTQDAIH
                     NFSNGNKNLFYISGKVKMNIPDLSFSTEDFKTSMELIEDSIIQTNCSFTNKIRMNNGP
                     NLPATIQDTYITQKIEYDFITGNMLIHQFSKTVNKWGAYHDDWTNTDKPEYTFEVEEK
                     DQKMWLKKILNLVPDTKSWSESTLLFETKTTQETMNTVEKLEESTKTTQFDASGKETE
                     SSSVHYINTDYTSDNKMHIWLHNK"
     gene            8076..9254
                     /locus_tag="PKH58_07480"
     CDS             8076..9254
                     /locus_tag="PKH58_07480"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX88908.1"
                     /translation="MKIKFFWLLLFSILLSGCKIINPPDDPDDPDDPDNPPIVAVDSL
                     GVSINTIAFTAGKDASLIIVRTNKSWTATKTADWLSLSATSGNKNTAFLIGAAPNDGL
                     MRETAVTIQAGDKTKEIKITQASAPRMTITVKGIPVNMILVEGREFTMGSSEQSSFGL
                     PHQVKLSDYYVSETEVTNALWLAVMGSLPYSDHSETDKLTLPVSEVNWNMVTGEFMPA
                     LNQLTGKIFRLPTEAEWEYAALGGKNNTGTKYAGSNTLDDVAWNMTNSGKQKKAVKGK
                     DPNQLGLYDMSGNVVEWCSDWYDYYYGFQIVNQSVVVPTLQTNPKGPDTGTNKIVRGG
                     SIESDEFWGFSACHVKYRYSIKPTGTDGCWGNTGNPQEPLCNFIKFTGFRLVIPVQNQ
                     "
     gene            9268..9885
                     /locus_tag="PKH58_07485"
     CDS             9268..9885
                     /locus_tag="PKH58_07485"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX88909.1"
                     /translation="MKKTIITSLALIAFMFCSAQTVVKMKMPPQAKDPLQVTVLFNEE
                     VPEGMPVVLGLMGYSVSGGITPYAYEWMQNGKVIGTGDVVVVTPAKGDKIELKATDKN
                     KCYSTMSFSMKVIAKIKNDEKDKEKEYKISPTMVKNGIVHITLPETESPIRANVRIFD
                     TNGAQVYQNQITGSTLITFDLPDGNYFISVKTDNYHKVGKIIVKN"
     gene            complement(10420..11166)
                     /locus_tag="PKH58_07490"
     CDS             complement(10420..11166)
                     /locus_tag="PKH58_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNX88910.1"
                     /translation="MNKSVLFVLVILLSIGCTPRKWSMTGYKTSKIAIDASSDSLADK
                     QMLAFIQPVKQELDKEMDQVVGFSEVEMKSDKPESLLSNWTSDIYRLAASEFLKQPVD
                     IAVVNMGSLRSQLPAGNLTVRDIFQLMPFENELVILWLKGSEVSRLLGIFAKEGGQGV
                     SGISMEIVDGKAQNVRVGGEPLDVDKLYSIATNDFLAGGNDRMVPLATPEKRVDTGLK
                     IRNILMEYVIRENRNGNKINAKLDGRITVR"
     gene            11306..>12299
                     /gene="tyrS"
                     /locus_tag="PKH58_07495"
     CDS             11306..>12299
                     /gene="tyrS"
                     /locus_tag="PKH58_07495"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNX88911.1"
                     /translation="MNFIEELEWRGMIHSMMPGTEEQLQKEMTSAYVGIDPTADSLHI
                     GHLVSVMMLRHFQRAGHKPIALVGGATGMIGDPSGKSAERNLLDETALRHNQECIKAQ
                     LAKFLDFESDSPNAAELVNNYDWMKNYSFLNFIRDIGKHITVNYMMAKDSVKKRLSSE
                     SSEGMSFTEFSYQLLQGYDFLNLYQTKNCKLQMGGSDQWGNITTGTELIRRKTGGEAF
                     ALVCPLITKADGGKFGKTESGNVWLDRRYTSPYKFYQFWLNVSDADAEKYIKIFTAIG
                     REEIETLIEEHRQAPHLRTLQKRLAQEVTVLVHSQEDYDAAVEASNILFGNATSE"
BASE COUNT         4031 a         2556 c         2384 g         3328 t
ORIGIN      
        1 acctttacac cttcctatgc tgtttctgat gctgcggtac gcggttctta tgctgtgctt
       61 atttttggcg tctattcggt taatgtgatg gggataaccc ttacaggtat tctagtctgg
      121 ctcattaatt tcgtaattcc catgctggcg ggcagttttt tcgtcctgaa agccaaatcc
      181 tggaagtcag ttgaaagccc ctcacaggaa tagaggtgca gtgaatttat tttttctcaa
      241 atgtcaggtc aattctaccc aagttcagtc cgcttttacc catctgagta atcagaattt
      301 tccgaccgga tacgttctcg gctgtaacat tttcgagtat tttgtgcgaa tgtccgccaa
      361 ctaccacatc gatgctgcgc gatgcagcca ccagatcgta atcggtgggt gaactgtctt
      421 cgtaagaggt gcccaggtgc gacagacaaa tcaccagatc acatttcagg ttatgtttca
      481 ggtaatctga agtttcagcg gcagttctga tcggatcgtt cagtatcagt ccggcaaagt
      541 ttttctgata tgccagtccc ctgagattca ccccgatacc taaaattcct attttcaatc
      601 cacccttacg aacaatgata aatggtttaa caaacggtgc aatcggcgta tttttcagat
      661 catagtttgc attcactatc ggcaacttca ccagtttcag tacggaagcc aatgtatcaa
      721 gaccgttgtc gaattcgtga tttccaagtg tcagggcatc gtaacccatc atctccagcg
      781 ctttggtttc aatgcgcccg ttgaagaaat tgaaataggg acttccctgc gaaaaatctc
      841 cggcatcaac cagcaacacg ttgggttctt cggagcgtat tttatttatt tctcccatac
      901 ggcgggcgta gcctcccata ttgttacttt cagcgggctc aacctgactg tgtgtatcgt
      961 tggtatgcag gatgacaatt ttagtttgtt tccctgcatt aactgacatt gaaaatgtta
     1021 tcagaataaa acagaaaaaa aatattttac ttttcatgcc tatcaaattt acttgtcgtt
     1081 tgttgtgttt tttattatct cctctttcca ttctctgaaa ttcatattgg tttttagctt
     1141 gacagctcgt ttcatagagt ctacagaacc aaatccggtc tgttcggcaa gctgatttaa
     1201 ggtgatgtcg ttatccgatt tcagtatttc ctcaatttca ttcagcctgt actgatttac
     1261 aaaggtacaa aaattctgct ggcaatgctc attgataact gatgaaatat aggtgcggtt
     1321 tgtcccgata gagtttgcta catcctgaat agtcacgcaa cttttcagat aaattttttc
     1381 ttgctcgaaa agtaccataa tttttttcag gatacttttc tcccttctcg aattacgtgg
     1441 cagttcgacc aaagcttctg tctgaaacgg aaattcttcg taagccggat tgacgggttt
     1501 ctgcctgttg cccaaccagc caatggtgaa aagcaacacc gaaaaaatca gggaaggaat
     1561 gcctaataat aggttgtgag caacaaaata gtcacggccc agaactgcaa ccacaatgga
     1621 cgtaagcgcc gtaaacagca tggacgcatt gagcaactgc acgctgatgg ttccactatc
     1681 gtgaacatcg gaataatact gtgcggcttt ataggcgtat tttcgtatca gaatggtgtt
     1741 tgcaaccagc acaagcacca cctgtatgac aaacacggct ttcatcagga caaatatcac
     1801 attgagccag gcgataccaa aggatgcgga aggaatatct ttctgaaaca gccatgattg
     1861 ataatcagct acgggagtta acataattcc gaataaatac agcaggaata atactataga
     1921 tggcaacaga taacgggcat gtttgcgtag agaaaacttc tcgtcgatag ttagcagcct
     1981 gaaataaatg taatacatag gaaataccat cagcgatgca aactgataaa tagcatctgt
     2041 ccaggcgtaa agtactttca gggaatcaaa gtacagaaaa tgcgaaaaat aaacgataaa
     2101 ggcaaaaatc atgaatttcc ccaggaaagt tctgggtgaa gtattcttgt caacatccgt
     2161 attcaacacg atggcccaaa acagcgtaac ataaagcgga cttattacaa acagggtttg
     2221 tgtcataaat tcatttgaaa atgggttaat cacatttact catcaactct ttacttattc
     2281 catttgcatt taaatatacc taccacaaat atagaaaaat aatttctcaa aaaaacaatt
     2341 tctatctatc aaaccggtat acaggttaaa tcttttaaca tctatgtctg ttttttttac
     2401 gaattaatga tatgttttta cgaattgcac atattttaat gcatttttct tctatttttg
     2461 tttaattaaa aacatctatt tagtttaatt aaaatctaat tagcatgata aagtttatat
     2521 gcatttcagt ttaactgaca atttttttta aatgaaaaac gatgattaga aaaacatatc
     2581 agcaacaaac aaacaaacag gctatgaata ctttacaata taatttccgg cgttcacaaa
     2641 caattttcgg gtttttcgta ttattgtttg tgtgtatatc aggttgcttc aatcagttgg
     2701 atggacaaat aaaatcaact gaagcagcag gcagatttgc cctgaactat tttaactcaa
     2761 cagcctataa aacaaagatg caaaaagttc cggcaagttc agccgatata gaactgaaat
     2821 atcaatcggg aggaaacgta caggcaccga tttatatatt tcaaaacgaa caaaggggtt
     2881 ttgtggtttt tgcacaaagc acacacggtt ttcagattct gggatattcc gacaaggcga
     2941 attttaatcc ggctgaaatt cctgacggtt tacagcaact gctgaaactc tacgaaaatg
     3001 ccagtccgga cagcatcatg gggatacaaa acaccagaga aaccacaaca gtggtgtcac
     3061 cattgctgga ccaggccgga gtgggactca accagtttca tcacgaaaat gtgggcggaa
     3121 gctggtccgg gtgtatggct acagccatga cacaaatcat gtgctattac aagtaccccg
     3181 acaaagggat aggatctcat tgttatacaa atgcggcata tggagagctt tgcgccgatt
     3241 ttgggaatac tcattacaac tggattaatc cctctgacga agatttcaaa ctattgtctt
     3301 atcatgtagg ggtatccatg gatatgaatt ataatggcag tccggcaggc agttcgccct
     3361 catctcctac ttctctctat gcattgcatg attattttgg atatacatgc gaaccattta
     3421 atgatttttc acgaaactca caagtaattc aaaacgttat tgctcagcaa aaacccgttt
     3481 atgctcatct cctgggcaat ccgggacacg ccgtggtact ggatggatat gacagcaacg
     3541 gattttttca tataaacttt ggctggggtg gcggtggaaa tggctactat atgcttaata
     3601 acaattcatt aatttatagt ggaaattata aattcggcac caacctggct gaagtcattt
     3661 tatatcagcc cggaccagtt cacctggata aaaccgattc gcttgcatta gtttcaataa
     3721 aaaataacat cagtaatctc gactgggatt tgtctgatgc tcaaaaaaga acgggcatta
     3781 caaccctcaa tggacgagta atgagtttga atatttactc tgcgaacatg attggcaatg
     3841 aaggttcgat acccgatgac atcgacaacc tgcatgaact tatcaactta aatatttcag
     3901 gtaaactgca tggaacgata cctcaaaaca tatcaaacct caccaacctt caatcactaa
     3961 ggatttcgaa cttctccggt acactgagcg atacaattcc aactaatatt ggaaatttaa
     4021 caaatctgac aaatttaagc atttttaatg ctgccaaagg accaattcca tcctcagtag
     4081 ggaaacttgt aaatttaaga agtcttaatt tgcctaccgg aaatatggat ggaacgatac
     4141 cggaagaaat ttactctctg aataatttgc aggaaataac tctcgacgat caaaaactgg
     4201 ccggaaatat ctctgaaagt ctgggaaatc tgaaaaacct gatattttta agcatcgcaa
     4261 acaatcaact aagcggaaaa atcccatcaa cgattggcaa cctgaacaag cttcaatatc
     4321 tcgatctgtc cggcaacaaa ctttcgggtg aaattcctcc ttcattaaat aattgcactt
     4381 cattatcaac cctgaaactt ttcagcaatg aactggaagg taaggtacct gcaatttttg
     4441 ataacaaatt taaactgacc agtctggatt taagcaacaa caaactcacc gaaatatccg
     4501 aaaacatagg caaattaacc aatcttcaga cattaaacct gaacaacaac cagctaagct
     4561 cactaccaga ttctctgaat gagctaaggg taatgaattc gttttcagcc agcaacaata
     4621 aaataaaccg attgcctgcc aacctgaacc aattgacaag cctgaatacc ctgaatctgg
     4681 taaataacgg aataactgtg ttcccaaatg acttgtgttt cctgccaaat ttaagtggga
     4741 ttgacctgag ttataataaa ataacccgat tgccggctat gatttccgac ttaaaggcac
     4801 aaatcctcca aattcaggaa aacgaacttt caggagcaat accggcaaat ctattacgta
     4861 ccgaaccggg ttattaccgt tttcaaaaca accggttcgt ttacaacgac ttgccacagg
     4921 gaaccgattt tacaaactta atcgggactc agaaaccgct taagctcctg aaaaacactt
     4981 tcaaaggcaa tctgggtgat tcaattgaaa ttgatatccg aaagatttgc agtaatttga
     5041 acaaacatga cacgtatgta tggtgcgaat atccgaaaga aaagaaagaa attcaaccaa
     5101 ctataaaaat agaagatggt ccggttttgc acctgaaatt atctgataaa aatatcggaa
     5161 aaaagtatta ttgcttaata acaaacgaca gcgcagctac ttacaaagac cctcagattt
     5221 attaccgaat tccgtgtctg agcaatctga ttaccgatac tttgtttctg gctatacaga
     5281 cggaagatga atttctgtca gagaaatatc ccgaacagta tattgtgaaa tcggaatcca
     5341 tgcaaaaaaa ggaactgagc gataaaaccg tcacgctaat ttcgcctttc aaaatgcgcg
     5401 gtataaagaa atgggaagca tcgtccaacc aaaaagaatg gtacgagtta tcggcttcca
     5461 tgacacagaa cgatctgaag gcaaatctgg tttcggtgaa tgaagaagaa ttgaaaattt
     5521 caccaaaaac tccggcttat taccgctgtg cactgttgga aacaaattgc gcgccccatt
     5581 acagcgatac gctgaaaatc aatccgtacg gaaaagtgat ttgcgacacc acactaaatg
     5641 tacagaacaa aacgattacg tttaagaaag acagcataga ggtaaccatt ccgcagggca
     5701 ttaacgacag cgacttcaga cttaccatcg tcaagcttga caatccaccg gctaagcccg
     5761 attctgtttt ccgcttaagt tccgtatatg atgtgacggt aagttttgga gacagttttt
     5821 atttaccggt tatcatcaaa ctaaaaaacc tgaataaaag gtcgtttaac cagatgaata
     5881 ttgataacta caaagctgtt tatttcaatg atattgaaca acaatgggag tattacaacg
     5941 atgcaaacat caatttgaat gacacaacac tcacattcgg cacttatcat tttacaaagc
     6001 tgggctactt cgagtttaac gactggaact atacacaccg gcttacccgt ggcagggtaa
     6061 acgtcattta caacgtaagt gatcaaagca tttttgatct ttatgatgga atggttgtaa
     6121 aagagggatt aaaaagctgg cacgatacaa ataccaatcc ggacaaggat ggtaatccct
     6181 atttaataca ggacattgcc gagtacatga accagattat aaataaatgt gatttactcg
     6241 ggcttgaaac accgggatta cgcttcaatg tctatgtaaa acaggtagga aattatgctc
     6301 agataggtgc agccagctat ctgtcgggac ggggatttct atatattgac ccaattttca
     6361 tgagtatgga aaaaacatat aaagaaaaca cggttcagtt gatgactact ctcgcccatg
     6421 aatactcgca ctatacgcag gactattaca tgtcgatgat tataaataat tatttctggc
     6481 aggaagccac agctcctttg ggtggcagaa tgatctggaa tacaactgat ttaccgctca
     6541 ctgaacctga gatgctgata aaacaaggat tatctccaac taaaggtaac aaaacgattt
     6601 tcgaggtact ttcagcatcc tggtttaatg attataactt accggttgtg agtaaacttg
     6661 cgtcaaattc ggccgattac aatcttgcaa gtctgtttct ccattacatg caaaattacc
     6721 gtcgcggaac aacgctggac gcggctgttc tcctgaaaga aacttcctat ttagatacct
     6781 ggctgggtta tctggaaagc tatattaaaa agtatctgaa gtcaaatacc ggaaccgaat
     6841 ttgccgatta cgtaaaatat attttcagcg gcgtcaatcc caattttacg gtcatcaacc
     6901 gggaagatgg aaatccatac aaatacatca ttcaaaatat tgacaataaa ggcgactttg
     6961 ctaatctgtt aaattataat ttcgataaac atgattatca aaccaataaa atttcaataa
     7021 aaatacccta cctgggagca agaatgtact tattgaacaa catgacgctg gacaaagctg
     7081 tagtggtaaa gtataaaaga ctgaccgaaa aaaacgaaaa cgaaaaaaca ttttatggaa
     7141 aatatgatga gaaaaccaaa cagatgaaaa tagtggaaat aacagattca acagaatatg
     7201 tttttctgct cgatgcactc actccaaaat caaataccga ttttctgaat tccggatttc
     7261 tgctttttgt caacgccaaa acaaacagta caatctttac cgattataat gcctccttcg
     7321 agttgtcagc catgccggta ccaaacatca cggctctatg ctatgcagat gtaacacagg
     7381 atgccattca taatttcagc aatggcaata aaaatctttt ttacatctca ggtaaagtta
     7441 aaatgaatat tccggatctg agtttcagca ccgaagattt taaaacgtcc atggagctaa
     7501 ttgaagactc aatcattcag acgaattgca gttttaccaa caaaatcagg atgaacaacg
     7561 gaccaaacct gcctgcaaca attcaggaca catacattac acaaaaaatt gaatatgatt
     7621 tcattacagg aaatatgctg atacatcagt tctccaaaac agtaaacaaa tggggcgctt
     7681 atcacgatga ctggaccaac accgataaac ctgaatacac atttgaagta gaagagaaag
     7741 atcagaaaat gtggctgaaa aaaatcctca atctggttcc ggataccaaa tcgtggtcgg
     7801 aaagtacact gctgttcgag acaaaaacca cacaagaaac aatgaatact gtggaaaaac
     7861 tcgaagaaag tacaaaaaca acccaatttg atgcgagtgg caaagagacc gaaagtagct
     7921 ccgtacatta tatcaatacg gattacacca gcgataataa aatgcatatt tggctacaca
     7981 acaaataatg tatgaaatca ccacgcctac aaatatcgac ggactaaaac cacataaagg
     8041 attaccattt gaacatctaa taaaaatata tgcaaatgaa aataaaattt ttctggcttc
     8101 tgcttttcag catactatta tcaggatgca agattatcaa tccgcccgat gacccggacg
     8161 atcccgacga cccggacaat ccgccaattg tagcagtcga ctcgcttgga gtttcaatta
     8221 ataccattgc ttttactgcc ggtaaagatg caagtcttat tatcgtacga acaaataaat
     8281 cgtggactgc tacaaaaact gccgactggt tatcactttc ggccacatcg ggcaacaaga
     8341 ataccgcttt tctcataggc gctgcaccca acgatggatt gatgcgcgaa accgcggtaa
     8401 ccatccaggc gggcgacaaa acaaaggaaa tcaaaatcac ccaggcaagt gcacccagaa
     8461 tgaccataac agtgaaaggg atccctgtca atatgatact ggttgaagga cgtgagttca
     8521 cgatgggaag cagtgagcaa agcagctttg gactgcccca tcaggtcaaa ctgagcgatt
     8581 actacgtatc cgaaacggag gttaccaatg ctctgtggct tgctgtgatg ggctcattgc
     8641 cttactccga ccacagcgaa accgacaaac tcacattgcc cgtaagtgaa gtaaactgga
     8701 atatggtaac cggtgaattt atgcctgcac taaatcaatt aacaggaaaa attttcagat
     8761 tacccaccga agccgaatgg gaatatgcgg ctctcggcgg caaaaataac accggtacca
     8821 aatatgcagg cagcaatacg ctggacgatg tagcctggaa tatgacaaac tcgggtaagc
     8881 aaaaaaaggc agtaaaaggg aaagatccaa atcagctcgg cctttatgac atgagtggaa
     8941 acgtagtgga atggtgcagc gactggtatg attattatta cggctttcag atagtgaatc
     9001 agagtgtggt ggttcccacg ctgcagacca atccgaaagg tcccgacacc ggaacgaata
     9061 aaatagtgcg tggtggaagc atcgaaagtg atgaattttg gggattttcg gcctgccacg
     9121 tgaaataccg ctactccatc aagcctacag gtactgatgg ctgctggggc aacacgggta
     9181 atccgcagga acctttgtgc aattttatca aatttaccgg cttcaggctg gtgataccag
     9241 ttcaaaacca ataaaacaca tactgtcatg aaaaaaacaa tcataacatc attagctctc
     9301 atagccttca tgttttgcag cgcgcagaca gttgtaaaaa tgaaaatgcc gccacaggcc
     9361 aaagatccgt tgcaggttac tgtattattc aacgaggaag tacccgaagg tatgcctgta
     9421 gtactggggc ttatgggcta cagcgtaagt ggaggaataa caccttacgc ctacgaatgg
     9481 atgcaaaacg ggaaggtaat cggaaccggc gatgtggtgg ttgtcacacc agcaaagggt
     9541 gataaaatag aattgaaagc taccgacaaa aacaaatgtt actccactat gtcgttctcc
     9601 atgaaagtaa ttgcaaagat aaagaatgat gaaaaagata aagaaaaaga atataaaatt
     9661 tcacctacaa tggtaaaaaa cggcatcgtt cacatcactc tgcccgaaac tgaaagtccg
     9721 atacgggcaa atgtgcggat ttttgacaca aatggtgcgc aggtgtatca gaatcaaatc
     9781 accgggagca cattgataac atttgattta cctgatggca attattttat atctgtaaaa
     9841 acggataatt atcataaagt gggaaaaatt atcgtcaaaa attaaactga ctatctaaac
     9901 cactcttaaa aaacagataa actttcgaaa aagaactaca gggattgatt tctcaatagc
     9961 acttgttttt tgataatgtt tgagtaagaa ttataactgt atgaatcatt aatctgcttt
    10021 atttactgaa aaatcatatt atttgaaaat catatagtgt cggcttttgc aaaaaagaca
    10081 tccgttagaa ttagtctttt gattatcagc gcaaatagca tttacagata tatctataaa
    10141 tactgcaact aaagaaatac aatgtaaatg tgaaatattt agcaactgag aaataaaaaa
    10201 tattttacga attacttgta ttattttacg aattacacat ccaattgata aagcatgaag
    10261 taactttgtt gaacaaataa ctttgttaaa taataattga tttagatact tatttttaat
    10321 aatataaaac ctaacctgtt tatctgttgt ttattcgcac acaaaagaag aattgcccaa
    10381 aaccccgggc aattctttct ttaaagtaca cagtattatt cagcggactg ttattctgcc
    10441 atctaattta gcgttaattt tattaccgtt acgattttcc cggattacat attccatcag
    10501 tatattgcgt atttttaatc cggtatccac tcttttttcg ggggttgcca atggtaccat
    10561 cctgtcgttt ccacccgcaa gaaaatcatt tgtggctatg ctgtagagtt tatcaacgtc
    10621 caggggctca cctccgacgc gcacgttctg cgctttacca tcaacgattt ccatacttat
    10681 acccgagact ccctgtccgc cttctttggc aaatatcccg aggagcctgc tcacttccga
    10741 acctttcagc cagagaatga ccaattcatt ttcaaagggc atcagctgaa aaatgtcgcg
    10801 aactgtgagg ttacccgccg gaagttgtga acgcagactt cccatattga ccacagctat
    10861 atccaccggt tgtttcagaa attcggatgc tgcaaggcga tatatatccg atgtccagtt
    10921 agacaacaaa ctttcgggtt tgtcggattt catttccact tccgaaaagc ccacgacctg
    10981 atccatttct ttatccagtt cttgctttac aggctggata aatgccagca tctgtttatc
    11041 agccagcgaa tcagaggaag catcgatggc aattttcgat gttttgtaac cggtcatcga
    11101 ccactttcgg ggtgtacagc caatggaaag taaaataaca agaacgaata gtacggattt
    11161 gttcatatcg ttaaggggat aaaataattg tcacgattgt catgcaaata taattaagtt
    11221 tttttgttcc gctacacgtt tccttctgtt tttcacttta tgaaaaaatg gctatctttg
    11281 cacttcgtaa ctaaaatata cacagatgaa tttcatagaa gaactcgagt ggcgtggtat
    11341 gattcacagt atgatgcccg gcaccgaaga acagctacag aaagaaatga cttcagccta
    11401 tgtgggcatt gacccaacag ccgattcact acatattggt cacctggtaa gcgtgatgat
    11461 gctcaggcat tttcaacgcg ccggtcacaa acccatagca ctcgtaggcg gagccacagg
    11521 catgattggc gacccttcgg gtaagtccgc cgaaagaaat ctgctggacg aaactgcact
    11581 tcgccacaat caggaatgca tcaaagcaca gctggcaaaa tttctcgact tcgaaagcga
    11641 ctcacccaat gcggccgaat tggtgaacaa ctacgactgg atgaagaatt attcttttct
    11701 gaactttatt cgcgatattg gcaagcacat cacggtcaat tacatgatgg ccaaggactc
    11761 agtaaagaaa cggttgagca gtgaatcaag tgaaggaatg tcgtttaccg aattttccta
    11821 tcaactcctg cagggatacg actttctgaa cctctatcag actaaaaact gcaaactcca
    11881 gatgggtggt tctgatcaat ggggcaatat cacaacaggt actgaactca tccgccgcaa
    11941 aaccggagga gaggcttttg cactggtttg tccgctgatt acaaaagccg acggcggcaa
    12001 gttcggaaaa actgaaagcg gaaatgtctg gctcgaccgt cgatatacat ctccctataa
    12061 attctatcag ttctggctta acgtcagcga tgctgatgct gagaaataca tcaaaatatt
    12121 cacagccatc ggtcgtgaag aaattgaaac tttgatcgag gaacaccgtc aggcacctca
    12181 tttgcgtaca ttacagaaac ggctggcaca ggaagtgacc gtactcgttc attcacagga
    12241 agattatgat gcagccgttg aagcatcaaa catattgttt ggcaacgcca cctccgaag
//
LOCUS       DAOPYB010000080        12291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5784_length_12291_cov_7.959709, whole genome shotgun sequence.
ACCESSION   DAOPYB010000080 DAOPYB010000000
VERSION     DAOPYB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5784_length_12291_cov_7.959709"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2301)
                     /locus_tag="PKH58_07500"
     CDS             complement(<1..2301)
                     /locus_tag="PKH58_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNX88912.1"
                     /translation="MKYKLNIRYKTNFNSPLGIVCFILFLFLLLSSCVSTMQLPKGEK
                     LYTGAVVKLESTQKINKKTIKSIEEKVIRPVPNKSYLGLRPKLWLYLLAGNEPYNAFE
                     RWLRKNGEEPVLISNVKPGATTSVIDAALYNNGIFNSYTQYEIAEKKHSAKVIYTSYV
                     HKPYVIKKLIYAIADDSISHIVLTDKENTLIKPKQNYSLATLVNERIRIDNLLKNKGY
                     YYFNPDYLLFKADTSTVDQSVTLKLTLKDSIPPEAKIVYYINNVYINQNYTLRRQRTD
                     SIRDTIMVQNTVFQGKRNEMNIRPGVILRSVYLKKNELYARQNHNITLSRLMSMGTFK
                     FVQVKFADSDTIPGFLDATILMTPMTNYTFRAELDLVTKSNNYSGPRLNLSLLNRNTF
                     KGGELLKLNMAGSYEIQLSGTNKNLYSYSLNPQAELTFPHFLVPFRLKQSNSIYNPRT
                     AFLVSFNYLRRGNYFDMRTFQFKYGFKWKNNIKIEHELNPVNISYSSIGNQSDAFIEK
                     LNKYPYLKKSYEEQLIAGASYSFTYNEQVVMGKKWQNYFHATAETAGNAFSLLKIIGG
                     DKPTSENPSKIIGSIYSQYAKLSLDGRTYYTFEDGNKVALRVFGGFARAYGNSAILPY
                     KKQFFSGGPNSIRAFQINSLGPGTYRREAGNIDFLQTGGDIKVETNAEYRFGIFRFIK
                     GAFFVDAGNVWLQKSNPSDIGTPFSFSDFMNEFAVGAGAGLRIDVSFFVLRFDLAVPL
                     RKPWLPENNRWVIDQINLSSPSWRREN"
     gene            complement(2314..7278)
                     /locus_tag="PKH58_07505"
     CDS             complement(2314..7278)
                     /locus_tag="PKH58_07505"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNX88913.1"
                     /translation="MKKILPYLKKIMKAIFWVVLIFVLLFLVVAGIIQIPAVQTKIVH
                     FATTFVSNKTHTKVEIKNVSIAFPKSVVLRGLYLEDLQKDTLIFAREANINIVLKDLF
                     LNKICVNNVELNDLTLNLHNTKTDSIFNYNFLISAFADTTLKVTDKTEIAVKWTFSLD
                     EVSLKNARVRYDDRFGGLEISASLDKLALSVNKIDFEKSVYNIDELLVDKLKVQLILT
                     KSSKTAVENRKNILPNIMASNIQINNSTLIFKDLINNQTAKGTIFRFELKNGTVDLEK
                     QTVELDRINLSESDIQYLTVRSEVVTDSIVKAVDKTQKSDWKVTVNNIDLANNTLTYQ
                     VVNAPRVKKGFDANFMKFRKLNLQAGDLFYSSDLTNVSIEKFNSIDQNNFAITRFETG
                     FIMDAHSITATKLKAVTTRSAVDADVYIHYSSLKSLVDSLPYLAMNMDIRNAKFINSE
                     ILYFSPELIKQDFFKNKTNITSLKGSISGRVNQLKGKNLIVKTGKNTVLETDFNIIGL
                     PKVETAFFDFPNLKIISGKQDINMFAGSAVPSSINLPENIALGIILKGKLKSFTTTIG
                     MKSSFGNADIEAKIDPQENFSGNMKVENFDLGSLLKNKEMYGPVSLTAQIDGRGLDLK
                     TTQARIKADITQLFLNKYNYKQLKIDGNVNGREFAGKVNLNDPNAVFDFDGLVNITPS
                     LEKYKFRLNVEALDLQRLNLSKKDIRIGFDASADLQGGTVDKMNGSAGISKMIVVSNG
                     KRYLLDSLLIATINKTGKSEVNLKSALIGIKYSGTITPFSLPAALSQFSNKYFPFTDA
                     KVATITNDSSNFNFEILLHNHPILSEVLLPELKTFEPGLITGSFDSRKSELKLNANMP
                     KIVYGSTEINNLVMDVNSDSTVLNYKIASDNLSNTQIKFENFLIDGKLADNKILANLS
                     SINKFQNKKLLIRTETTKENGNFRLKFDPKDFWLMNNRWDVAADNYIEFGKPGFLIHH
                     LFMNNGESQIQVASVHDKFNDDLNIGIRNFKLDDISRIVEKDTNIVKGLVDGNVLLKR
                     VNKSYGIIADASISNLIFREVPVGNLTLKADNPSGERFEVDMKLFGNENKLSASGYFV
                     PNGGENSMNINVAIDSLSMKTVEAFSMGQLSESSGMLAGNFQVGGKTSAPELTGELTF
                     NNAFLKPAYLNNRLELKHETLQLKTDGIYFNSFTLLDMNQHKATIDGSVKMKQFSDFV
                     FALNINTRNFLLLNTTAKDNEAFYGRLIVDSRIDVKGPMSLPLINGRVKLRDDSNFTF
                     VVPEDKLTTDKGEDVVEFEDSIPSVSILDRKDKKVVQKSGFKGFELSSVIEVDKEATL
                     RLLLDPASSDSLVVRGDAALSFTMDRSGNMDLTGTYHLNEGSYLVSLESVIKRKFDIV
                     TGSTITWNGDPLDADVSIDATYAVRAAPYDLVVEQMAGLTDVEKGGYKQRYPFLVLLK
                     LRGAILKPEISFEIKLRPEDIGILDGEVNQKIVTLNEDPSLLNKQVFALLVLGRFVQE
                     NPLQTESGGTSSLIRSSVSNFLSTQLNKLSSKVITGVDLNFDIQSYDDYETGKAQGRT
                     QVEIGLKKQLFDDRLSVQVGGSVDVEGDKAKQNSTSDITGDITVEYKLTKDGQYRLKG
                     FRHNQYEGAIEGQLVETGVGVVLVKDFNKWKEKNPQRLKGNKKK"
     gene            complement(7601..10585)
                     /locus_tag="PKH58_07510"
     CDS             complement(7601..10585)
                     /locus_tag="PKH58_07510"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNX88914.1"
                     /translation="MNTLKIKMAAKQLLFAFLIVIGYCPAAISQDYFQQQVNYKIDVS
                     LNDTLHELSAYEEVNYTNNSPDTLRFLYFHLWPNAYSTNKTPLARQIFAFKGKQKLFN
                     DPELRGYIDKLNFKVNNLTVSWNLLPNQPDICKIMLNSPLLPGKNIVITTPFHVKIPK
                     GVTSRLGHIGQTYQITQWYPKPAVYDRHGWHPMSYLDQGEFYSGFGRFDVSITLPENY
                     VVAATGILQDSTELKMLDALVADTTWKSIKMLGKVKHITPTSTKKTLHYTGENMHDFA
                     WFADQQFNVMKGKVILPETGKEVTVWVMFTSRQSRLWRNALKYTSRAILDFSKMIGDY
                     PYTCYTLVQSPLSAGLGMEYPGLAVIGPTKNAWTLDNVIVHEIGHSWFYGALGFNERR
                     YPFLDEGLTSNYENREMTRKYPDKKLWEVLLKKKKQAKFLHADKFPLEELEELEWLIP
                     ARNNTEQPLDLPSTDYNRINYGQMVYNKTAMGFTYLRAYLGDTVFDAAMHDFYRQWKF
                     RHPEPDDLRAAFESQTSNSLSWFFDDFISTTKRLDYKLVKLDKQQLLVKNKGEMISPL
                     IIAGLSGDSVVFEKWVDGFSGEKWIEIPTGDYTELKIDPNHVMPELYRLNNNLRTSSL
                     FPKADPVQPQLLVGIEDPEKIPLMYIPAVNWNHENGFMVGAALYNGLITPKPVEYFLM
                     PFYSFNQSRLAGFGRIAYNITPYNKLIRIATLSLQGAQFGAPGQRDYRKIQAGLDILF
                     TKNNSANPIQHGMYGRFALASDLYDILNQQQAPLNRYVQLGYNLQKSSLVNPYRLLAS
                     FEAGTAFSKTAVDFDYTQSYSGRDNGLKLRFFAGAVLKNTSSTSFYSLAPAGRSGREL
                     YLYEGTYPNRFGVFPTSIWSRQMTLSEGGLVSPVNAALGYSQWLLSLSLSSSLPGMLS
                     KTGIKPFVNLLYNDHGLSTGYNSPFFMEAGLKAGIMNIFEIYVPFFVSNNIQSITGPV
                     KDRIRFVLNLDISKASKAGGGN"
     gene            complement(10691..11002)
                     /locus_tag="PKH58_07515"
     CDS             complement(10691..11002)
                     /locus_tag="PKH58_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88915.1"
                     /translation="MKKAIFLALLTILSLGAAPAFAAKGSADKTVATEATLTAEEVNA
                     LKARVIEIRDMDKSELTTAEKSELKGELKEIKETIQNEPYIYIGGVTLLLIIILLVIL
                     L"
     gene            complement(11023..11175)
                     /locus_tag="PKH58_07520"
     CDS             complement(11023..11175)
                     /locus_tag="PKH58_07520"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HNX88916.1"
                     /translation="MLTLIPIILAITWFIGFFIFNAGYFIHILLVLAIVAALFRLTRG
                     IEINEY"
     gene            11426..11950
                     /locus_tag="PKH58_07525"
     CDS             11426..11950
                     /locus_tag="PKH58_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88917.1"
                     /translation="MKKIKLLIVCVSILIAGFAQGQVAVNVNIGTPPAWGPAGYSDVR
                     YYYLPDMEVYYDVQRSNFIYLHDNRWVYRTSLPVQYKNYNLYRGYKVPLQNYSGNYPF
                     FFFKQHKVKYAKGYKGNPQKNIGSKSNKQVNKFIKSQHGKSNGNMKMNKQHGNNGNKG
                     YKGNKGNKGNGKNK"
     gene            11960..>12291
                     /locus_tag="PKH58_07530"
     CDS             11960..>12291
                     /locus_tag="PKH58_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88918.1"
                     /translation="MWFVNGKKQAVSQLKQFVSSIYLIQKTVYKFILVKTTVYPELKA
                     KFTTYTFVKCFKYRTKNKEQRTKTKEPELQNITHYTLHTTHYTSHITHHTLHITHYTL
                     HITHYTLH"
BASE COUNT         3518 a         2500 c         2337 g         3936 t
ORIGIN      
        1 gttctctctc cgccacgaag ggctgctcag attaatctga tcaataaccc atcggttatt
       61 ttccgggagc catggcttac gcaaaggcac ggccagatcg aagcgtaaca caaagaaaga
      121 cacatcgata cgcaatcctg caccggctcc tactgcgaat tcattcataa aatccgaaaa
      181 tgaaaatggt gtaccgatat ccgatgggtt tgatttttgc agccatacat ttccagcatc
      241 cacaaaaaaa gcgcccttta taaagcggaa aatcccgaag cgatattctg catttgtttc
      301 cactttaatg tctcccccgg tttgcagaaa gtcaatattc ccggcctccc tccgatatgt
      361 tccgggacca agcgaattta tttgaaatgc acggatacta ttcggtccgc cgctaaaaaa
      421 ttgtttcttg tagggcaata ttgccgaatt tccatacgcg cgggcaaatc caccaaagac
      481 tcttaaagcc accttattgc catcttcaaa ggtgtaatac gtccggccat cgagacttaa
      541 tttggcatat tgcgaatata ttgaaccaat gatttttgaa ggattttcgg aggttggttt
      601 atcaccgcct ataattttta ataatgaaaa agcattaccg gcagtttcag ctgttgcgtg
      661 aaagtagttt tgccattttt ttcccattac gacctgttcg ttgtaagtaa acgagtaact
      721 tgcgcctgca attaattgtt cttcgtagct ttttttgagg tagggatact tatttagttt
      781 ctcaatgaat gcatcagatt ggttgccaat ggaagaatag ctgatattta ccggatttag
      841 ctcgtgttca attttaatgt tatttttcca cttaaaaccg tatttgaact gaaaagtcct
      901 catatcaaaa taattacctc ttcttaaata attaaacgat actaaaaagg cggttctggg
      961 attataaatg ctgttggact gttttagtct gaatggcacc agaaaatggg gaaaagtaag
     1021 ttcagcctga ggattcaacg aataggaata caggtttttg ttcgttccgc tcaattgtat
     1081 ttcatatgat ccggccatat ttaactttaa taattcacct cccttgaatg tgtttctgtt
     1141 caacaaactc aaattcaaac ggggtccgga ataattattt gattttgtaa ccagatccag
     1201 ttcagcccga aacgtataat tagtcatggg tgtcatcaaa atggtggcat ctaaaaaacc
     1261 cggaattgta tcactgtcgg caaacttcac ctgcacgaat ttgaaagtac ccatcgacat
     1321 caggcggctc aaagtaatat tgtgattttg ccttgcatac aattcatttt ttttcagata
     1381 aaccgaccgt aaaattacac cgggacggat attcatttca ttcctttttc cctgaaaaac
     1441 agtattttga accataatgg tatcacgaat gctgtcagtt cgctgtcttc tcaaggtgta
     1501 attctgatta atatacacat tattaatata ataaacgatt ttagcttccg gcggaatact
     1561 atcttttaac gtcagcttta aagttacaga ttgatcgacg gttgaggtat cagctttgaa
     1621 tagcaaataa tcaggattga aataataata ccctttgttt ttcaacaagt tatcaattcg
     1681 tattcgttcg tttaccaacg tagccagact ataattctgc ttcggtttta tcaacgtgtt
     1741 ttctttatcg gtaagaacga tgtgactaat tgagtcatca gcaatcgcat aaatgagttt
     1801 ttttattaca tacggcttat gcacgtaact tgtataaatc actttggcgg aatgtttttt
     1861 ctccgctatt tcatattgag tataactgtt gaaaatgccg ttgttgtaga gtgcggcatc
     1921 aatcacggag gtggtagcac ccggtttcac attacttatc agcaccggtt cttctccgtt
     1981 ttttctgagc catctttcaa atgcattgta aggttcattg cccgccagga ggtataacca
     2041 caatttggga cgcaaaccca gataactttt atttggaacc ggtcgtatta ctttctcttc
     2101 aatggatttg attgtttttt tgttaatttt ttgcgtagac tccagtttta caactgcgcc
     2161 ggtatatagt ttttcccctt tgggcagttg catggtactg acgcatgaag aaaggaggag
     2221 gaaaagaaac aaaataaaac acacaattcc caaaggggag ttgaagttag ttttgtatcg
     2281 tatgtttaat ttatatttca tgtgtttttt aatttatttt ttcttgttcc cctttaggcg
     2341 ttgggggttt ttctctttcc atttattaaa atccttaacc aggaccactc ccacaccggt
     2401 ttctactaat tgtccttcaa tagctccctc gtactgattg tgtcgaaaac ctttcaaccg
     2461 atattgtccg tctttggtca gtttgtattc tacagtaata tcgccggtga tgtcacttgt
     2521 cgaattttgt ttggctttat caccttccac atccactgaa cctccaacct gaactgataa
     2581 ccggtcgtcg aaaagttgct ttttcaatcc gatttctacc tgtgttcttc cctgtgcttt
     2641 cccggtttca taatcgtcat acgattgtat gtcgaagttt aaatccaccc cggtaattac
     2701 ttttgaactt aatttgttta gttgagttga cagaaaatta ctcaccgatg aacgaatcag
     2761 ggaggaagtt ccacccgatt cggtttgtaa tggattttcc tggacaaacc ttcccaacac
     2821 caataatgca aaaacctgtt tgttcagtaa agagggatct tcattcagcg taactatttt
     2881 ttgattaact tctccatcca aaattccgat atcctcaggc cgcaatttaa tttcaaagct
     2941 aatttcgggt ttaagtattg caccgcgaag ttttaataat accagaaaag ggtatctttg
     3001 tttgtatccg cctttttcca catcggttaa cccggccatt tgttcaacta caaggtcata
     3061 aggtgcagcc cgtacggcat aagtcgcgtc aatggaaaca tcggcatcca atggatcgcc
     3121 gttccaggtg atggtacttc ctgtcacaat atcaaatttc cttttaataa ccgattccaa
     3181 tgaaaccaga taactgcctt cattcaggtg ataagtaccg gtcaaatcca tatttccgct
     3241 cctatccatg gtaaaactca aggcagcgtc tcctctcact accagcgaat cggatgaagc
     3301 cgggtctaac aataagcgca gggttgcttc tttgtcaact tcgattaccg atgaaagttc
     3361 gaatccctta aatcctgatt tttgaacaac ttttttatct ttgcggtcga gaatagagac
     3421 agatggaatt gaatcttcaa attcaaccac atcttcgcct ttgtcggtag tgagtttatc
     3481 ctccggaact acgaatgtga aattagaatc atctctcagt tttacacgtc cgtttattaa
     3541 cggcaaggac ataggtcctt ttacatcaat tctactgtca acgatcaatc gtccgtaaaa
     3601 ggcttcgtta tctttggcag tggtgttcag taataaaaaa ttacgtgtat taatattcag
     3661 tgcaaaaacg aaatcggaga attgtttcat tttaactgag ccatcaatgg ttgctttgtg
     3721 ctgattcata tcaagcaggg taaatgaatt gaaatagatt ccatccgttt ttagctgcag
     3781 cgtttcatgt ttcagttcaa ggcggttatt cagatacgcc ggtttgagaa atgcattatt
     3841 gaaagtcagt tcgccggtca gttcaggtgc tgaggttttt ccacctacct gaaagttacc
     3901 tgcaagcatt cccgaagact cggatagctg tcccatcgaa aatgcttcta ctgttttcat
     3961 cgagagtgaa tcaatcgcca cgttgatatt catggagttt tcgcctccat tcggaacaaa
     4021 ataaccactg gccgacagct tgttttcatt accgaacaac ttcatgtcca cctcaaatct
     4081 ttccccgctg ggattgtcag ctttcagggt taaatttcca accgggactt ccctgaaaat
     4141 caggttcgag atactggcat ccgcaatgat tccgtacgac ttattaaccc gtttaagcaa
     4201 cacatttcca tccaccagcc ctttaacaat gttggtatct ttttcgacaa tccgcgagat
     4261 gtcgtctaat ttgaagtttc ttatcccaat gttcaggtca tcgttgaatt tatcatgaac
     4321 cgaagcgacc tgtatctgac tttcgccgtt gttcataaat agatgatgaa taagaaatcc
     4381 cggcttgcca aattctatgt aattgtctgc tgctacatcc cagcggttat tcatcagcca
     4441 aaaatctttc ggatcgaact tgagtctgaa atttccattt tctttggtag tttcagtacg
     4501 tatcagcagt tttttgttct gaaatttatt tatggatgac aaattcgcca gtattttatt
     4561 atcagctaat ttcccatcta tcaggaagtt ttcaaatttt atttgtgtgt ttgacaggtt
     4621 atcactcgcg attttatagt tcaataccgt tgaatcggaa ttcacgtcca tcaccagatt
     4681 atttatttcg gtacttccat acactatctt tggcatgttg gcattcagct tcaactcact
     4741 tttgcggcta tcaaaactac ccgtaataag tcccggctca aatgttttta gctctggtaa
     4801 aagaacttcc gaaagtatgg gatgattgtg aagcaggatt tcgaaattaa agttggatga
     4861 atcgtttgta attgttgcca ctttggcatc cgtaaacgga aaatatttgt ttgaaaattg
     4921 agaaagtgcc gccggcaggg agaacggagt tattgttcca ctgtatttta taccgataag
     4981 tgcacttttc aggtttacct cgcttttacc tgtcttgttg atagtggcta tcagcagcga
     5041 atcgagcaaa tatcttttac cattgctgac gacaatcatt ttagaaattc cggcactgcc
     5101 attcatttta tcaactgttc ctccctgaag atcggcgctg gcatcaaatc caatccgtat
     5161 gtcttttttg ctcagattta gcctctgaag gtcaagcgcc tctacattca accgaaattt
     5221 atatttttcc agagatggag taatgttcac caaaccatcg aaatcaaaaa ctgcgtttgg
     5281 atcattcagg ttgaccttcc ccgcaaactc ccgaccgttg acatttccat caattttcag
     5341 ttgtttgtaa ttatatttat tcagaaatag ttgagttata tctgccttta tcctggcctg
     5401 cgttgttttt aaatccagac cgcgtccatc aatctgagct gtgagcgaca ccggaccata
     5461 catctcttta ttcttcaaca agctgcccag gtcaaaattt tcaactttca tgttgccgct
     5521 gaaattttcc tgagggtcaa ttttagcttc aatatcagca tttccaaaac tacttttcat
     5581 gccgatggtt gtagtaaaag atttcaattt tccttttaga ataataccca aggctatgtt
     5641 ttcgggaaga tttatgctgc tgggaactgc tgagcccgca aacatattga tatcctgctt
     5701 tcccgaaatg attttcagat taggaaagtc aaagaatgca gtttcaacct ttggcagtcc
     5761 gataatattg aaatcagttt ccaataccgt atttttaccg gttttcacta tcagattttt
     5821 ccctttcagc tgattgacac gtccggaaat gcttccttta agggaagtaa tgttcgtttt
     5881 gtttttaaaa aagtcctgtt tgattagttc cggactgaaa tacagaattt cggagtttat
     5941 gaacttcgcg tttcttatat ccatattcat agccagatac ggaagtgaat ctaccagtga
     6001 tttaagcgaa ctataatgta tataaacatc agcatcaaca gccgaacgag tggtgacggc
     6061 cttcagtttg gtggcagtga tggaatgagc atccataatg aaaccggttt cgaagcgagt
     6121 gatggcaaag ttattttgat cgattgaatt gaatttttca atgcttacgt ttgttaaatc
     6181 agaagaataa aacaaatcac cggcctgcaa attcaactta cgaaacttca tgaaattagc
     6241 atcaaaaccc ttcttaaccc ggggagcatt aacaacctga taagtgagtg tgttatttgc
     6301 gaggtcaatg ttgtttaccg taactttcca gtcacttttt tgggttttat ctacagcttt
     6361 tactattgag tccgtaacca cttcggacct tacagttaga tactgaatat cactttctga
     6421 taaatttatc ctgtctaatt ccactgtttg tttttccaga tctacagttc cattttttag
     6481 ttcaaaacga aagatcgttc cctttgcggt ttgattgttg attaaatcct tgaatatgag
     6541 ggtggaattg ttgatttgaa tattagatgc cattatgttg ggcaaaatat ttttcctgtt
     6601 ttcaactgct gttttcgagg acttagtcag gataagctga acttttaatt tatcaaccaa
     6661 taactcatca atattataaa ccgatttttc gaaatcaatt ttattcacac tcaaagctaa
     6721 tttgtcgagc gaagcagaaa tttccagtcc accaaaccta tcatcgtagc gtacgcgtgc
     6781 atttttcagg ctcacttcat cgagcgagaa tgtccatttt acggcaattt cagttttatc
     6841 tgttactttc agtgtggtgt cggcaaaagc agaaatcaaa aaattataat tgaagatcga
     6901 atcggtttta gtattgtgca ggttgagtgt aaggtcgttg agttcaacgt tgtttacgca
     6961 aatcttgttt aaaaataaat ctttaagcac aatgtttatg ttggcttccc gggcaaaaat
     7021 cagggtatct ttttgcaaat cttcaagata aagacctctg agaacaactg attttgggaa
     7081 tgcaatgctt acatttttaa tctccacttt tgtatgggtt ttgttgctta caaaagtggt
     7141 ggcgaaatgt acaatcttag tctgaacagc cggaatctga ataattccgg ccactacgag
     7201 aaataaaagc acaaaaataa gtaccaccca aaagatagct ttcataatct tctttaaata
     7261 gggaagtatt tttttcattt tgatagatat ttagtctgat cagacttttt attcataaat
     7321 tcattttcag tttttgatct caatgatttt attttgcatt gattatacgt attttcaaat
     7381 aatggtataa aatttcaatt cccttaatca taaatgtcgg gatcaaaatt gaaacagaaa
     7441 atttttataa ctgcctgatt tgtttagcat taaaaaaaaa aaattatttt caatattaca
     7501 ttgtctgttt gcgacagcac tgtgctgttc catttgatta tttgaaagtt tttgatatct
     7561 catttacata ttatgttgtt gaattcatct tatctcaaat tcaatttccc ccacccgctt
     7621 ttgaagcttt cgagatgtcc aggttgagca caaaccgtat ccggtctttc accggtccgg
     7681 tgatggactg aatgttattg ctgacaaaga aagggacgta aatttcgaaa atattcatga
     7741 tacctgcttt taagcctgct tccatgaaaa agggagaatt gtaaccggtt gagagtccgt
     7801 gatcgttata caacaagttg acaaacggtt taattccggt tttggacaac attcccggca
     7861 aactgcttga aagcgaaagt gacagaagcc actggctgta tccaagagcc gcgttcaccg
     7921 gtgaaaccaa accaccttcg gaaagtgtca tttgccgcga ccagatggaa gtgggaaaca
     7981 ccccgaaacg attgggatac gtgccttcgt aaagataaag ttcccgcccg cttcttcctg
     8041 cgggtgcaag agaataaaag cttgttgatg atgtgttttt cagcacggca ccggcaaaaa
     8101 aacgtaattt caatccatta tctctaccgg aataactttg tgtataatcg aaatccacag
     8161 cggttttgct gaatgcagtt cctgcttcga aagaagccag caacctgtat ggattgacca
     8221 aactcgattt ttgtaaattg tatcccaact gcacatagcg gttcagcggt gcctgctgct
     8281 gatttaatat atcataaaga tcggatgcca gcgcaaaccg tccgtacatg ccgtgctgaa
     8341 tgggattggc agagttgttt tttgtgaata aaatatccag gcctgcctgt atttttcggt
     8401 agtcccgttg gcccggagcg ccaaactgag ctccctgcaa actcagagtt gcgatgcgga
     8461 tcaacttgtt gtagggagtg atgttgtaag ctattctccc gaaacccgcc aatcgggatt
     8521 gattgaaaga atagaatggc atcaggaaat attccactgg ttttggtgtt atcagtccgt
     8581 tgtaaagcgc cgcacccacc ataaatccgt tttcatggtt ccagttaaca gccggaatgt
     8641 acatcagggg aattttctcc gggtcttcga tacccaccag caactgcggt tgcaccggat
     8701 cagctttggg aaaaagactg gaagtccgga gattgttgtt gaggcggtag agttcgggca
     8761 ttacatgatt gggatcaatt ttcagttcgg tataatcacc ggttggaatt tctatccatt
     8821 tctcacccga aaaaccatcc acccattttt caaaaaccac actatccccc gaaagtccgg
     8881 ctattattaa tggagaaatc atctcaccct tatttttgac aagcaactgt tgcttatcta
     8941 attttaccag tttatagtca agccgtttgg tagtgctgat aaaatcatcg aaaaaccagc
     9001 ttaaactgtt ggaagtttga ctttcaaatg cagcgcgcag gtcgtcgggt tcgggatgtc
     9061 ggaatttcca ttgccggtaa aaatcatgca tggctgcatc gaaaacagta tcgcccagat
     9121 acgccctcag ataagtgaaa cccattgccg ttttgttgta aaccatttgt ccgtaattta
     9181 tccggttgta atctgtcgag ggcaggtcga gcggttgttc ggtgttgtta cgtgccggaa
     9241 taagccattc aagttcttcc aactcttcca aaggaaattt atctgcatga aggaatttcg
     9301 cctgtttttt ctttttcagc aacacttccc agagtttctt atcaggatat ttgcgtgtca
     9361 tttcccggtt ttcatagttg ctggtcagcc cttcgtcgag gaaaggataa cgacgttcgt
     9421 tgaatccaag tgcaccgtaa aaccacgaat gaccgatttc gtgaacaatc acattgtcga
     9481 gcgtccatgc atttttggtg ggtccgatga cggctaatcc cggatattcc attcccagtc
     9541 cggcactcag cggactttga accagcgtat aacaggtgta cggataatcg ccaatcattt
     9601 tcgagaaatc cagtatcgcc cgactggtgt atttcaacgc atttcgccat aatcgcgatt
     9661 ggcgcgaagt aaacatgacc cagacagtca cttcttttcc cgtttcggga agtataactt
     9721 tccctttcat cacattaaat tgttgatcgg caaaccaggc aaaatcgtgc atgttttctc
     9781 cggtataatg cagtgttttt ttagtggagg ttggagttat atgtttcact ttgccgagca
     9841 tcttgatact tttccaggtt gtgtcggcaa caagcgcatc gagcatttta agttcagtgc
     9901 tgtcttgaag aattccagta gcagccacca cgtagttttc aggcaatgta atgcttacat
     9961 caaatcgtcc gaacccggaa taaaactcac cctgatcgag gtatgacatg ggatgccagc
    10021 catgccggtc gtaaactgcc ggtttgggat accattgggt gatttggtag gtctgaccaa
    10081 tatgccccag gcgggaagtt actcctttgg ggattttgac atgaaaaggg gtggtaatta
    10141 caatgttttt gcccggaaga agcggcgaat tgagcatgat tttgcaaata tccggctgat
    10201 tgggaagtaa gttccagctc acagtcaggt tatttacctt aaagttcaac ttatcaatat
    10261 accctctcaa ctcaggatca ttaaatagct tttgctttcc cttgaaagcg aaaatctgtc
    10321 gggcaagcgg agttttattc gttgaatagg cattgggcca caggtggaaa tacaaaaacc
    10381 gaagcgtgtc gggcgaattg ttggtgtaat tcacttcttc gtatgcgctg agttcgtgaa
    10441 gcgtatcgtt tagcgataca tcgattttat aattcacctg ttgttgaaaa taatcctgcg
    10501 aaatagctgc cggacaatac cctataacaa tcagaaaagc aaaaagaagt tgcttagctg
    10561 ccatttttat tttcaatgta ttcatttttc agataatatt tttttctaca agaaaaacag
    10621 ggtcttgtaa gaattacaaa accctgcttt ttttcttgcc attttaacgg atgtactacg
    10681 aaagaattga ttacaataaa ataaccaaaa ggataatgat aagcaacaat gtaactccac
    10741 ctatatagat ataaggttcg ttctggattg tttcctttat ttctttcagt tcacctttca
    10801 gttcgctttt ttcagcggtt gtcaattccg atttgtccat atcgcgaatt tcaataacac
    10861 gtgctttcaa cgcatttact tcttcggcgg ttaaagtagc ttctgttgca actgtcttat
    10921 cagctgaacc tttggctgca aatgccggtg ctgcacctaa cgataatatc gtcagtagtg
    10981 ccagaaaaat tgcttttttc atttttggtt tttttaaaat tattagtact cattgatctc
    11041 tatgcctctg gttaggcgaa acaaagctgc tactatcgct aacactaaaa gtatatgtat
    11101 aaaataaccg gcattaaata tgaaaaatcc aataaaccat gtaattgcca aaatgattgg
    11161 aatcagtgtc aacataattt tcataattta ttgtttttag attgtcttgt ttgcttctac
    11221 ctcgaataaa ttgatgtaaa aatcaataat gtaaagatag agctgaccac gactaaaatc
    11281 cttacacttc gcttggaaaa tgttgtatat ttcacagatg caattccaat gtgtgaataa
    11341 tgtaacattt tgggggattt gtgtaactta ttcggggtca gcaagcctta tttttgtgct
    11401 acaaaaaccg tttaaatact tagttatgaa aaaaataaaa ttactgatag tttgtgtatc
    11461 gatactgatt gccggttttg cacagggaca ggtcgctgtg aatgtaaata ttggtacacc
    11521 acccgcctgg ggtcctgccg gatattccga tgtgcggtat tattaccttc ctgatatgga
    11581 ggtttactac gatgtgcaac gttccaactt tatctacctt cacgacaacc gatgggttta
    11641 ccgaaccagt ttgccggtgc aatacaaaaa ctacaatctg taccgcggct acaaagttcc
    11701 gcttcaaaat tacagcggta attatccgtt cttctttttc aaacagcata aagtgaaata
    11761 tgctaaagga tataaaggaa atcctcaaaa aaacattggc agtaaaagca ataagcaggt
    11821 aaacaagttt atcaagtctc agcatggaaa aagcaatgga aatatgaaga tgaataagca
    11881 gcatggaaat aacgggaata aaggatacaa gggcaacaaa gggaataaag ggaatggtaa
    11941 aaacaaataa tgattttgaa tgtggtttgt gaatggtaag aaacaagctg tttcgcaatt
    12001 gaagcagttt gtttcttcaa tttatttaat tcaaaaaacc gtttataaat tcattcttgt
    12061 gaaaacaact gtttatccgg aattaaaagc taaatttaca acttatactt ttgtaaagtg
    12121 ttttaaatac agaacaaaga acaaagaaca aagaacaaag acaaaagaac cagaactcca
    12181 aaacattaca cactacacac tacacactac acattacaca tcacacatca cacatcacac
    12241 attacacatt acacattaca cattacacat tacacattac acattacaca t
//
LOCUS       DAOPYB010000081        12270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5792_length_12270_cov_10.215473, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000081 DAOPYB010000000
VERSION     DAOPYB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12270
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5792_length_12270_cov_10.215473"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..242)
                     /locus_tag="PKH58_07535"
     tRNA            complement(157..242)
                     /locus_tag="PKH58_07535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(207..209),aa:Leu,seq:taa)
     gene            complement(264..336)
                     /locus_tag="PKH58_07540"
     tRNA            complement(264..336)
                     /locus_tag="PKH58_07540"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(301..303),aa:Gly,seq:gcc)
     gene            complement(466..1440)
                     /locus_tag="PKH58_07545"
     CDS             complement(466..1440)
                     /locus_tag="PKH58_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNX88919.1"
                     /translation="MSFIDKIRQPILAELQEFENVFNTVLKSDNTLLSNVHEYVMQGH
                     GKKMRPILTILAAKLFGEVNQSTLYGAFSLELLHTASLVHDDVVDDTMERRGRPSVNA
                     KWTNKVAVLSGDYILSKSLHSATVTHNLRILEAIATIGMILPDGELLQLANTEQTALS
                     EDKYLDIIKRKTAMLFATCTEVGGLSVNVDEKSLNHLKKYGEYLGMCFQIKDDIFDYY
                     EDAQVGKPTGNDVRDGKLTLPLIFALRNTNGTEKDEIVKMIDNKDFSTANINKIMQFA
                     HNNGGVDYAISRMNDFKKMAMDELTDLPDNEIRTSLHECVEFVTDRDF"
     gene            1703..4534
                     /gene="polA"
                     /locus_tag="PKH58_07550"
     CDS             1703..4534
                     /gene="polA"
                     /locus_tag="PKH58_07550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNX88920.1"
                     /translation="MKKLFLLDAYAIIYRAYYAFIRNPRVNSKGLNTSAVFGFVNTLE
                     DVLKREKPTHIAVAFDPKGKTFRHEAYEHYKAQREMTPEDIRLAVPIIKEIVKAYNVP
                     VLEVPGFEADDVIGTLAKKAEQNDFEVYMLTPDKDYGQLVSEHIFMYRPKHSGGYEVM
                     GPDEVKAKYELDSHEQVIDLLGLMGDSSDNIPGCPGVGEKTAVKLLQEFGSIDNLLNN
                     TDKLKGALKTKIEQNKEQIVFSRFLATIKTDVPIEFEEERYLTEPVNETELRALFDEL
                     EFRTLSARIWSAGKANVEKQETKPPANKPVHGQMSLFDAPAPVSAQETQPDLFSQLSD
                     MQTVAGMRNIHEVSHHYVLVSDKAQRAGLITKLFNQKSVCFDTETTGIDVFSADLVGL
                     SFCFTAGEAYYVPLPTGREECMLVLKEFKAFFQSEQIEKIGQNIKFDLLMLASYGIEL
                     RGKMFDTMIAHYLLQPELRHGMDYLAEIYLKYRTIHYEDLFGGKPKTQQNILNVDLNL
                     LCDYAAEDADITFQLKQILEKELKNNGLEKLFYEMEMPLMRVLAVMEQNGVRIDIEAL
                     RQSSLILTKDMQELEKEIHTLAGYEFNVSSPMQVGEILFDRLKLDEKAKKTKTGQYST
                     SEDVLEKLRSKHPVIGKILDYRGLKKLLSTYIDALPQLVNPKTGKVHTTYNQTVAATG
                     RLSSTNPNLQNIPVRDELGREIRKAFIPEEGENLLSADYSQIELRIMAHLSGDANMIN
                     AFNSGYDIHAATAAKIYKVPIEEVTSDMRRKAKTANFGIIYGISTFGLSERLNIPRAE
                     AKELIDSYFLTYPDVKKYMDDVIQRAKELGYVETIFGRKRYLSDINSQNSIVRGFAER
                     NAVNAPIQGSAADIIKIAMIRIQDELEKRKLKSKMTLQVHDELNFSVPQIELDEVREL
                     VIREMEQAIALKVPLIAECGVGANWLEAH"
     gene            complement(4614..4868)
                     /gene="rpsT"
                     /locus_tag="PKH58_07555"
     CDS             complement(4614..4868)
                     /gene="rpsT"
                     /locus_tag="PKH58_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNX88921.1"
                     /translation="MANHKSSIKRIRQTEKRKLANRYYAKTARNAVRKLRATTEKSAA
                     AEMLPKVSAMLDKLAKRNVIHKNKAGNLKSKLAVYVNKLA"
     gene            complement(4901..4972)
                     /locus_tag="PKH58_07560"
     tRNA            complement(4901..4972)
                     /locus_tag="PKH58_07560"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4937..4939),aa:Glu,seq:ttc)
     gene            complement(5272..5589)
                     /gene="trxA"
                     /locus_tag="PKH58_07565"
     CDS             complement(5272..5589)
                     /gene="trxA"
                     /locus_tag="PKH58_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX88922.1"
                     /translation="MALEFTDENIKDFINSGKPVVIDFWAEWCGPCRMVGPLVEELAE
                     EYAGKVEIGKMNVDENIDTPNEYGIRNIPTILFFKDGVLADKQIGATPKATLKSKVDA
                     LLS"
     gene            complement(5735..9535)
                     /gene="dnaE"
                     /locus_tag="PKH58_07570"
     CDS             complement(5735..9535)
                     /gene="dnaE"
                     /locus_tag="PKH58_07570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNX88923.1"
                     /translation="MSSFVHLHVHSSYSVLDGMSSISGLVDKAIASGMNALALTDHGN
                     MFGIKEFYNTCKKKNAKTKDKIAELEKKLKSDGITDEEKETIGKEIELTKQKIFKPIF
                     GCEVYVARRSRYDKDEKDDRSGYHLVLLAKNETGYHNLCRLVSYGWVQGFYYRPRIDH
                     DLLAKHSEGLIASSACLGGEIHKNIEAGDLVAAERAVLWYKKIFGDDYYLELQRHKTD
                     KPGADTQTYVKQELQNKDLISLARKTNTKLIASNDVHFVEEEHADAHEHLICLSTGKD
                     LDDPTRMRYTKQEWLKTPEQMAEIFADIPECLENTVEIANKVEHFKLEAPPMMPVFPI
                     PEEFGTEEEYRQKYDETILREEFGDAFDRIGGYDKAIRIKLDADYLTKLTYEGAMRRY
                     GADMNTETKERIDFELDVMKKMGFPGYFLIVQDFIQAARDMNISVGPGRGSAAGSVVA
                     YCLKITNIDPMKYDLLFERFLNPDRISLPDIDIDFEDDKRGQVVRWVTEKYGKGRVAN
                     IVTYGTMGTKSAIKDVARVQKLPLSEADRLTKLIPGKFPKDENDKDIKVNLTNCFKYV
                     DELRDARESSDPVLSNTLKYAEMLEGTVRQTGVHACGLIIGADDLINLVPLSTAVDKE
                     TKEDKVITQFEGTVIEDVGLVKMDFLGLKTLSIIKDALSNIKKSKGIDIDIDSIPIDD
                     EKTYHLFSKGDTVGVFQFESAGMQKYLKELKPSKFEDLIAMNALYRPGPMDYIPQFIR
                     RKHGEEPIEYAFPVMEKRLNDTYGITVYQEQVMLLSRDLGGFTRGQSDELRKAMGKKL
                     KDKMESLHEKFLQGAQKNGYKDVEKLEQIWSDWAKFASYAFNKSHATCYSLVAYQTAY
                     LKAHFPAEFMAANLTRNKDDITEVSKLMDECKSMKMLVLGPDINESELTFTVNEDGNI
                     RFGLGGIKNVGEGAVEAIIEERKKNGKFKNNFDFFERVNLNACNKKNVESLVLAGAFD
                     SLPDIRREQFFTLNSREEQVLDTFMKYGNLYQQDVSFNQNSLFGALEDAVEITKPEVP
                     YTPEWSPLVKLNKEREMIGMYLSAHPMDEFEFELNNICNANTVDIKDPSKYQLGTGLK
                     IGGMITAFRDGMTRTGKPYGMITVEDYHGSHEFPLFGKNFEDYGKYMIKDLFIYIHAL
                     VQEKGADYKYQPTRDPNVKPEIELKIANIMPFKDVRKLIKRIHINLSLQKLSADVVEE
                     LTRVIAGNKGDVNVYIEVKDFFSSEKVTLFARSHRIKINSAVYKYLKYAEEEDILRYK
                     IDMS"
     gene            10000..11697
                     /locus_tag="PKH58_07575"
     CDS             10000..11697
                     /locus_tag="PKH58_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88924.1"
                     /translation="MLIYISVVTAALLFSACNRQRNDADLMHLNKIDSLLTIQPEAAA
                     ESLKQLHPVKLSHYNRGYYQLLKVIALDKTYYNFTSDSFINATVHILTRHKQMYPRTY
                     ARSLMYQGLVRYRMGVTDSTAYEPIKEAADLLEMKKIKDPLLLYFCYHYLGLIHNENN
                     NSSYSIKYYEKAIKEIKEYGDKNYLFNTYTEITWAYLKINENYIAKQYIDTLNNFKNI
                     SDTQQANINQILSIYYECTNEFQKALEINKKILRSNNNYKDLSRMLFKISENYQALNK
                     LDSALLYAEKAEMCKQDSSYYLNYFYYKNIGEISEQLRLWQKSAAAYKKAYLLKEKAT
                     TKELDKQVMSLEKKYDLNEAENKALLYRNSNILITIIAVFLMVLLVTLLIIWKQRNKQ
                     LHLKQLLNEQKTMALEKNNQLLKERQQRLQMEKERTDQQIIEQQFLLPLYEQITTRNT
                     QIRKFLYDLTFDTHISKMPFLHEKVKSEFKLFDKASHAKNFESLTDEYFSKITHFTEE
                     ELRSLNKSEKLLMSLLVMKVPHQQISVLLNTTSESLSTRKLKLKKKMELNDMKMDDI"
BASE COUNT         3651 a         2434 c         2500 g         3685 t
ORIGIN      
        1 tgtaattgca aaactattaa tttttattca atccacaact tttacgggtg atccgcaatt
       61 attagagtgt atgctatagt acaaaatagt tttgtgcaaa tgttgtgcaa acaaaaaacc
      121 ctaactgatt gaaaatcaat tagggttttt cttttgtggt acccggaggg ggacttgaac
      181 ccccacagcc gtgaagccaa aggattttaa gtccttcgtg tctaccattc caccatccgg
      241 gcagattgct cttacaagaa tcgagcgaaa aacgggactc gaacccgcga ccctaacctt
      301 ggcaaggtta tgctctacca actgagctat tttcgcaatg ttttatagaa actaacgctt
      361 tactcgttcg ttaagcgggt gcaaatatac atactttttt ttaatgtcaa aataatgttc
      421 tgaaaaaaac tcatggaatt actgttgtaa tactatttat ccaggtcaga aatcacggtc
      481 agttacaaac tcaacacact catgaagcga tgtccttatt tcattgtccg gtaaatccgt
      541 cagttcatcc attgccattt ttttgaagtc attcatgcga ctgatggcat agtccacacc
      601 accattgtta tgcgcaaact gcatgatttt gtttatattt gctgttgaaa agtctttatt
      661 atcaatcatt ttcacgattt catccttttc tgtgccattt gtgtttctta atgcaaaaat
      721 taaaggcagg gtgagttttc cgtctctgac atcattaccc gttggtttac caacctgagc
      781 atcctcgtaa taatcaaaaa tgtcatcctt aatctgaaaa cacataccca ggtattcccc
      841 atatttcttc aaatgattaa gagacttttc atccacatta accgataatc cgccaacttc
      901 ggtgcaggtt gcaaacagca tggctgtttt tcgcttgatg atgtccaggt atttatcttc
      961 ggataaggca gtttgctcgg tatttgcaag ttgcaggagt tctccgtcag gcaaaatcat
     1021 cccgatggtc gcaatagctt ccagtattct gagattgtga gtaacagtgg cggaatgaag
     1081 cgatttggat aaaatataat cgccgcttaa cactgcgact ttgtttgtcc atttagcatt
     1141 taccgaaggg cgtccgcgtc gttccatggt atcgtccact acatcgtcgt gtaccagact
     1201 ggctgtgtga agcagttcga gtgaaaatgc accgtataaa gttgactggt ttacttctcc
     1261 gaaaagtttg gcggcaagaa tggtgagtat tggtcgcatc tttttcccgt gaccctgcat
     1321 aacatactcg tgtacattcg acagaagggt gttgtcactc ttgagcacag tgttgaacac
     1381 attttcaaat tcctgcaact cggcaagaat aggctgtctg attttatcga taaaactcat
     1441 ttgaacattt aataatagtc cgaattgcaa aaataaacaa ttttactgtt tgtttcattc
     1501 aatcgggtca aatgattgtc tttttttgtt taattttacg gtctgataga atttttgctg
     1561 aataatatta atcatttctg caacttctct tcaacaaaat agaacacttg cggattgaaa
     1621 taaacaattt gaatttgttt atgttctgaa tattttaccg cttaatctga acattatttt
     1681 ttattgcaaa aagacaaacc gaatgaaaaa acttttttta ctggatgcat acgctatcat
     1741 ttacagagct tattacgcat ttattcgaaa tccgagggtg aattcgaaag gtctgaacac
     1801 ttcagccgta ttcggatttg ttaatacgct cgaagatgtt ttgaaacgcg aaaaaccaac
     1861 gcatattgca gtagcttttg atccgaaggg gaaaaccttc aggcacgaag catacgagca
     1921 ttacaaggcg caacgggaaa tgacaccgga agatatccga ttagctgttc caatcattaa
     1981 ggaaatagtg aaggcttaca atgttcccgt actcgaagtc cccggatttg aggcagatga
     2041 tgttattggc acactggcaa aaaaagcaga acagaatgat tttgaagtgt atatgcttac
     2101 tcctgataaa gattacgggc aactcgtttc tgaacatatt ttcatgtacc gacccaaaca
     2161 cagcggcggc tacgaagtaa tgggacccga tgaggtgaaa gctaaatacg aactcgatag
     2221 ccacgaacag gtaatcgatt tactggggct gatgggcgat agttcggata atattcctgg
     2281 ctgtccgggc gtaggtgaaa aaactgctgt taaattactt caggaattcg gcagtataga
     2341 taatctgctg aataacactg ataaactgaa aggtgcgctg aaaacgaaaa tagaacagaa
     2401 caaagagcag attgtctttt cacgctttct ggcgaccatc aaaaccgatg ttcccatcga
     2461 gtttgaagaa gaaagatatc ttacagagcc ggtaaatgaa acggaattac gcgctttatt
     2521 tgatgaactg gaattccgga cactttcggc ccgaatctgg agtgccggaa aagcaaatgt
     2581 tgaaaaacag gaaacaaaac ctcctgctaa taaaccggta cacgggcaaa tgtcgctgtt
     2641 cgatgctccc gctcctgtaa gcgctcagga aactcaaccc gacctgtttt cacagctgtc
     2701 cgatatgcaa accgttgccg gaatgcggaa cattcatgaa gtgtctcatc attatgtact
     2761 ggtcagcgat aaagctcaac gagccgggtt gataaccaaa cttttcaatc agaaatcggt
     2821 atgttttgat accgaaacca ctggtattga tgttttttct gccgatctgg tgggtttatc
     2881 gttttgtttt accgcaggag aagcttacta tgtaccactg ccaaccggca gagaagaatg
     2941 tatgctggta ttgaaggaat tcaaggcttt ctttcaaagt gaacaaattg aaaaaatcgg
     3001 gcagaacatt aaatttgatt tactgatgct ggcttcttat ggtattgaat tgcgcggaaa
     3061 gatgtttgac accatgattg ctcattacct gctccagccc gaattgcgtc atggaatgga
     3121 ttatctggct gaaatatacc tgaagtatcg tactatacac tacgaagatc tttttggagg
     3181 gaagcctaaa acccagcaaa atatcctgaa tgtagatctg aatttgcttt gtgactacgc
     3241 tgcagaagat gcagatatta cgtttcagct gaaacaaatc ctggaaaaag aactgaaaaa
     3301 taacggactc gaaaagcttt tctacgaaat ggaaatgcca ttgatgcgcg ttttggctgt
     3361 gatggagcaa aacggtgtaa gaattgatat tgaagccctc cgccagagtt cactcatact
     3421 gaccaaagat atgcaggaac tcgaaaagga aatacacaca ctggccggat atgaatttaa
     3481 cgtaagttca ccgatgcagg ttggggagat acttttcgac cgcttgaagc tggacgagaa
     3541 agcaaaaaag actaaaaccg gacaatattc cacctcggaa gatgtgctcg agaagctcag
     3601 atcgaaacat ccggtaatag gtaaaatact cgactatcgc ggactaaaaa aactgctcag
     3661 cacctatatt gatgccctgc cacagttggt aaacccgaaa acaggaaagg tgcacaccac
     3721 ctacaatcaa acggtagcag ccacaggccg gctgagttcc accaacccaa acctccaaaa
     3781 tataccggtg cgtgacgaac tcggacgtga aatacgcaaa gcatttatcc cggaagaggg
     3841 tgagaacctg ttgagtgccg actattcaca gattgaactg cgcatcatgg cacatctcag
     3901 tggtgatgca aatatgatca acgcgtttaa cagcggatac gatattcatg ctgctacagc
     3961 tgcaaagatt tataaagttc cgatcgaaga ggtgacatcc gatatgcgca gaaaggctaa
     4021 aactgcaaat ttcgggatta tttacggcat atccacattc ggactgagcg aacggctgaa
     4081 tatcccacga gctgaagcca aagagcttat cgacagttat ttccttacat atcccgacgt
     4141 gaagaaatac atggatgacg ttatacaacg cgctaaagaa ttgggctacg tagaaactat
     4201 atttggcaga aaaagatatc tctctgatat taattcacag aacagcattg ttcgcggatt
     4261 tgctgaacgt aacgcagtca atgctcccat acagggatcg gctgctgaca ttataaaaat
     4321 tgcaatgatt cgaattcagg acgaactgga aaaaagaaaa ctcaaatcga aaatgaccct
     4381 gcaggtgcat gacgaattaa acttcagtgt gcctcagatt gaactggatg aagttagaga
     4441 gctggttata agagaaatgg agcaggctat tgcgctgaaa gttccactga ttgccgaatg
     4501 cggggtagga gctaactggc tggaagccca ttagcagtat gtttgcagaa agatacgaca
     4561 aagaagccct ttctcatttg agaaagggct tctttattat atttcagaag caattaagct
     4621 aacttgttta cgtaaacagc aagttttgat ttaagattgc cggctttgtt tttgtgaata
     4681 acattgcgtt tagcaagttt gtcaagcatg gcacttactt tgggcaacat ttcagcagca
     4741 gccgattttt cagttgttgc acgcagtttg cgaacagcat tacgggctgt ttttgcgtaa
     4801 taacggtttg ccagtttccg tttttcagtc tgacgtattc ttttaatgga tgatttgtga
     4861 tttgccatct caatctgttt tttaagtctg ttatttatag tagcccatag gggaatcgaa
     4921 cccctctttc cagaatgaaa atctggcgtc ctaaccgata gacgaatggg ccattgaacc
     4981 ggatttttta agatatcctt gttttcaatt gcgggtgcaa atatacggca gttttttgaa
     5041 ttggcaaata cctgtcggga aatattttga aaatttcttc gattaaatct gtaatatgtt
     5101 gatatgcagg gataaaaacc atgcaataaa atatataaac tttgtttatt cctttgctct
     5161 gttctccgat ggacaataat tttaacgttt atttcctgaa aaaattaatg ggatgaaggt
     5221 cgtttcatcc cattaacgtt caaatgtgtg ataaaattaa taaacagtag gtcatgaaag
     5281 cagtgcgtca actttagatt tcagagttgc tttcggtgta gcaccaatct gtttgtctgc
     5341 cagtacacca tctttaaaga aaagaatggt tgggatgttg cggataccat actcgttggg
     5401 agtgtcgatg ttttcatcta cattcatttt cccgatttct actttgccag catattcttc
     5461 ggcaagttct tcaaccagtg ggccaaccat gcggcatgga ccgcaccatt cggcccaaaa
     5521 atcaataaca accggttttc cggaattgat aaaatcctta atgttttcgt ctgtaaattc
     5581 taatgccata attttatgta ttttataatt gtttgtttta ttaattaaag taataacgca
     5641 gttgtaagta aaaaagtttt tcgggtttat atgattgagg tgttaaaatg gtgatacata
     5701 tctaataagt caataggttt tcttaaactg aagcctaact catatcaatt ttatatctca
     5761 gaatatcctc ttcctcagca tatttcagat atttatacac tgccgaattg atctttatcc
     5821 ggtgtgatct ggcaaatagt gtaacctttt cagaactgaa aaagtccttt acttcaatat
     5881 atacatttac atcaccctta ttgccggcaa taacacgggt cagctcttcc accacgtcgg
     5941 cagatagctt ttgcaacgaa aggttaatgt ggatgcgctt gataagtttt ctcacgtctt
     6001 tgaatggcat gatattagcg attttcagtt ctatctccgg ttttacattc ggatcgcggg
     6061 tgggctgata tttataatcc gcaccttttt cctgaacaag agcatggata taaatgaata
     6121 agtccttaat catgtatttt ccgtaatctt caaagttttt tccaaagagc ggaaactcat
     6181 gcgaaccatg gtaatcttca acagtaatca tgccataggg ttttccggtt cgggtcatac
     6241 cgtctcggaa ggcagtgatc atacctccaa ttttcaggcc tgtacccagc tgatatttgg
     6301 aaggatcctt gatatctacc gtgttggcat tacatatatt gttgagttca aattcaaatt
     6361 catccatcgg atgtgccgaa agatacatac caatcatttc gcgctcctta ttaagcttga
     6421 ccagcggtga ccattcggga gtgtaaggta cttccggttt tgtaatttcc acggcatctt
     6481 ccagtgctcc gaagagggag ttctggttaa atgagacatc ctgctgatag aggttaccat
     6541 atttcatgaa cgtatcgagt acctgttcct ctctggagtt gagcgtaaaa aattgttcac
     6601 ggcggatatc tggcagggag tcgaatgcac ctgcaagaac caaactttcc acgtttttct
     6661 tgttacaggc gttgagattt acccgttcaa agaagtcgaa attatttttg aatttaccat
     6721 tctttttgcg ttcttcaata atagcttcca ctgcaccttc tcccacattc ttgatacctc
     6781 cgagaccgaa acggatattc ccatcttcgt tgacggtgaa ggttagttca ctttcgttga
     6841 tatccggacc gagtactagc attttcatgg acttgcattc gtccatcagt ttgctcactt
     6901 cggtgatgtc gtctttattt ctggttaagt tggcagccat aaattctgcc ggaaaatgag
     6961 ccttgaggta agcagtctga tatgcaacca gcgagtaaca ggtggcgtgc gatttgttga
     7021 atgcgtagga tgcgaatttt gcccagtccg accagatttg ttccagcttt tctacatcct
     7081 tgtatccatt tttctgggct ccctgcagga atttttcatg cagcgattcc attttatcct
     7141 tcagtttttt acccatagcc ttacgcaact cgtcggactg accgcgggta aatccgccaa
     7201 ggtctcggct caagagcatg acctgttcct gataaactgt aattccgtag gtgtcgttta
     7261 gtcttttttc cattaccgga aaggcatatt cgatgggttc ttcgccatgc tttcggcgaa
     7321 tgaactgtgg aatataatcc attggcccgg gacggtacag ggcgttcatg gctatgaggt
     7381 cttcgaattt ggaaggtttc agttccttga ggtatttctg cataccggca gactcaaact
     7441 ggaacactcc gaccgtgtcg cccttactga aaagatgata tgttttttca tcatcgatgg
     7501 gtatgctgtc gatatctata tcaatgcctt ttgatttttt gatgttgcta agggcgtctt
     7561 tgatgatgga cagggttttc aggcccagga agtccatttt taccaatccg acatcttcaa
     7621 ttacagtacc ttcgaactga gtgataacct tgtcttcctt ggtctccttg tccactgcag
     7681 tactgagtgg taccaggttg atcagatcgt cggctccgat gattagtccg caggcatgaa
     7741 cgccggtttg gcgcacggtg ccttcgagca tttccgcata tttcagggtg ttggaaagca
     7801 ccggatcgct ggattcacgg gcatcgcgga gttcgtccac atatttgaaa cagttggtca
     7861 gattgacttt aatatccttg tcgttctcgt ctttggggaa cttgccgggt ataagcttgg
     7921 tgagtctgtc agcttcggag agtggcaatt tctgcactcg agctacgtcc ttgatggccg
     7981 atttggttcc catggtgccg taagttacga tgtttgccac acggcctttg ccatatttct
     8041 cggtaaccca ccgtacaacc tgtccacgtt tgtcatcttc gaagtcgatg tcaatatcag
     8101 gtagtgaaat acggtcgggg ttgaggaaac gctcaaacag caggtcgtat ttcatcggat
     8161 cgatgttggt gattttcagg cagtatgcca ctactgatcc tgctgcggag ccacgtccgg
     8221 gtcctaccga aatattcata tcgcgtgcag cctgaatgaa atcctgtaca atgaggaagt
     8281 aacctggaaa acccattttc ttcatcacat ccagttcaaa atcgatacgt tccttcgttt
     8341 cggtattcat atccgcgcca tagcgacgca tagcaccttc gtaggtgagt ttggtcaggt
     8401 aatcagcatc aagcttgatc cgaatggcct tgtcataccc gccaatacgg tcgaatgcat
     8461 cgccgaattc ttcgcgcagg attgtttcat cgtatttctg acggtattct tcttctgtgc
     8521 caaattcttc agggatgggg aaaacgggca tcatcggagg cgcttcaagc ttgaagtgtt
     8581 ccaccttatt ggctatctcc acggtattct caagacattc aggaatatct gcaaaaattt
     8641 cggccatttg ttccggtgtc ttgagccatt cctgttttgt gtagcgcatg cgggttgggt
     8701 cgtccagatc ttttcccgta ctcagacaga tgagatgttc gtgagcgtcg gcatgctctt
     8761 cttctacaaa atgaacatca ttcgacgcga tgagtttggt gttggttttg cgtgcaaggc
     8821 tgattagatc cttattttgc aattcctgtt ttacataggt ttgagtatca gctccgggtt
     8881 tatcggtttt atgtcgctgc agctcgaggt agtaatcatc tccaaatatt tttttatacc
     8941 acagcacagc cctttcggca gcaacaaggt cgccggcttc aatatttttg tgaatttcgc
     9001 cacccaaaca ggccgatgac gcaataagtc cttcgctgtg ctttgccagc agatcgtggt
     9061 ctatacgggg acggtaataa aatccctgaa cccacccgta ggatacgagg cggcagagat
     9121 tgtggtatcc ggtttcgttt ttggcgagta gtacaaggtg gtatccgctt cggtcatcct
     9181 tttcatcctt gtcgtatcgg ctgcgacgcg ctacatacac ctcacaacca aatataggtt
     9241 tgaatatctt ttgtttcgtc agttcaattt ccttaccgat tgtttctttt tcttcatccg
     9301 taatgccgtc tgatttcagt tttttttcca gttctgcgat tttatctttt gttttcgcat
     9361 ttttcttttt acaagtgttg taaaattcct taatgccgaa catattgccg tgatcagtga
     9421 gtgccagggc attcattccg cttgcaatgg ctttatctac caaacctgat atgctcgaca
     9481 tgccgtcgag taccgaatag ctggagtgaa cgtgtaaatg tacgaatgaa ctcatttgtg
     9541 taatgatctg gaagtaaacg atttaaaaaa gagtattttt tggaaaaatc cggctgataa
     9601 aggtacaaga atatttatcg ggaagaaaat tttcacacaa aaagttattg acaatttatc
     9661 ggaaaacagt atgaaatgat gtgaatgagg aagatagacc atattaaatt ttcaatacaa
     9721 aaatattgtc tgacgattca ttatgataat gaaagtctgt tctgttttca aatagttcca
     9781 acaaacaaaa gctgactaat acaaacactt ccggataata tgccattcgg gtgaatattg
     9841 aaatcatttt aatagaaaaa cgaaacttta atcttaacac tttatattga agtgactgtg
     9901 gagaatgtgt gttttttgtg ttttattatt gcttgttaaa ttttaactat tatttttgtg
     9961 agttaaaaac agcaaaaatc caacaagatg accaggcgaa tgttgatcta tatatctgta
    10021 gtaaccgcag cgttgttgtt ctcggcgtgt aaccggcagc ggaacgatgc cgacctgatg
    10081 catctaaata aaatagacag tttgttgacg atacagcctg aagcggcagc agaaagcttg
    10141 aaacaattac atcccgtaaa actctcacat tacaaccgcg gttattatca acttctaaag
    10201 gtgatagcgc tggataagac ttattacaat ttcacatccg acagctttat aaatgcaaca
    10261 gttcacatcc tgacgcgcca caaacagatg tacccgcgca cctatgcccg ttcgttgatg
    10321 tatcagggac tggtgcgtta ccgcatggga gttaccgata gtacagccta cgagccaatt
    10381 aaagaggcag cagatttact tgaaatgaaa aaaatcaagg atccgctctt attgtatttt
    10441 tgctatcatt atctgggctt aattcacaac gaaaacaata attcatcata cagtataaaa
    10501 tattatgaga aagcaattaa agaaataaag gaatacggtg ataaaaatta tctgttcaat
    10561 acctatactg aaattacctg ggcgtatttg aagattaatg aaaattatat tgccaaacag
    10621 tatattgata ctttaaataa tttcaaaaac atatcggata cacaacaagc caatataaat
    10681 caaattttgt caatatatta cgaatgtact aatgaatttc agaaggcgtt ggaaattaac
    10741 aagaagattc taagatcaaa taataattac aaggacttgt ccagaatgtt atttaaaatc
    10801 tcagaaaatt atcaggcatt aaataagtta gacagcgctt tgctgtacgc tgaaaaagct
    10861 gaaatgtgca agcaggattc aagttactat cttaattatt tttattataa aaacataggt
    10921 gaaatttcag agcaactcag gttgtggcaa aaaagtgctg ctgcatacaa aaaagcatac
    10981 ctattaaaag aaaaagcgac aactaaagag ttggataaac aagtaatgtc acttgaaaaa
    11041 aaatacgatt taaatgaagc tgaaaataaa gccttacttt atagaaacag taatatcctg
    11101 ataacaatca ttgctgtgtt tttgatggtg ctacttgtga cactcctgat aatatggaag
    11161 caaagaaaca agcaattgca tttgaaacaa ttgctcaatg aacagaagac aatggcgctc
    11221 gaaaaaaaca atcaactgct caaagagcgt cagcaaagat tgcaaatgga aaaagaaaga
    11281 accgatcaac agataataga acagcagttt ttacttccgc tctatgaaca gataacaacc
    11341 cgaaacaccc agattagaaa atttttgtat gatcttactt ttgatacaca tatcagtaag
    11401 atgccttttt tgcacgaaaa ggtaaaaagt gagtttaaat tatttgacaa ggcgtcccat
    11461 gcaaaaaatt ttgaatcttt gacagatgag tattttagta aaattaccca tttcaccgaa
    11521 gaagaattac gttcactcaa caaaagcgaa aaattgctga tgtcattatt ggtgatgaaa
    11581 gttccacatc agcaaatcag tgttttattg aacactacct ccgaaagttt atcgaccaga
    11641 aagctgaagt tgaagaaaaa aatggaatta aacgatatga aaatggacga tatttgaaga
    11701 attacacatt tcgaacaacc attttgaaca tcaactttca ttatgctata tgtctgtaaa
    11761 tgatgtatat agatatcaaa ttcacatcaa ctttttgtcc tgccttgcaa aacccttgca
    11821 aggtattttt gtttccggta atttagctcc ggcaaatgga atgaaaatta tacccaaaag
    11881 aagtaataac tgacaaagtt aaataagttt gttttcgctc agtaaagtga ttgatatatt
    11941 tcactatttt tcaaagctaa acaggtggga tacgcattca catatcatct gtaaagcaaa
    12001 gactattgtt ttttcaggat aaaatcaaat acaatatgct ggatatcttc acactgtaat
    12061 aatgcctgca ataaaacaag gatgagcagg gtggcagaaa gagtgcacta aaggagattt
    12121 tactcataat caaaaagata aaaaggaaac tattcagtaa cgacacaaat accattaaac
    12181 caattataat ttatctgttt tgttcatgtt gtttttaaaa gtgttcattt aattaaattc
    12241 cggtcaatca accgatcgtc ctgtccctaa
//
LOCUS       DAOPYB010000082        12172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5850_length_12172_cov_8.361641, whole genome shotgun sequence.
ACCESSION   DAOPYB010000082 DAOPYB010000000
VERSION     DAOPYB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12172
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5850_length_12172_cov_8.361641"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKH58_07580"
     CDS             complement(<1..235)
                     /locus_tag="PKH58_07580"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNX88925.1"
                     /translation="MSFIAEKIQFEGFTFDDVLLVPSYSEVLPRDVDLTTRFTRNIEL
                     KIPIVSAAMDTVTEAKMAIAIAREGGIGVIHKNM"
     gene            complement(388..1104)
                     /locus_tag="PKH58_07585"
     CDS             complement(388..1104)
                     /locus_tag="PKH58_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX88926.1"
                     /translation="MKKAIVVGASSGVGRELAKTLAENSYKVGITARREYLLQELAAE
                     KPDAYVVKPFDLLETDIVLTKLDELVDELGGLDLLVISAGLIKLNTDLDYNVEYKINM
                     VNVTAFSMMCNWAFNLFRKQGYGHLAGITSVAGLRGWRNNPAYNASKAFQMNYLEGLK
                     NLAHYLKLPITVTNIIPGYMETDMKGKTLVFWVCPPEKAARIIFKALCKRQKIIYVYR
                     RWRFTAFLYKHLPNKVIECA"
     gene            1338..2171
                     /locus_tag="PKH58_07590"
     CDS             1338..2171
                     /locus_tag="PKH58_07590"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX88927.1"
                     /translation="MKEEIKKVRIDFDQLASTLFAGKKIPRLLMRILKKIAHEDGLNE
                     LFDSAPGKKNMDFVDACMDFYRIECRVKGIENLPADAKPLIFASNHPLGALDAISILH
                     SIGHRYDGKIKFYANEILNHVEPLKEMVLPVSKYSYQGRNNVRKVQEFFRSSEHLLIF
                     PAGATSRKYGDKIIDLEWQKNFIQKSVHYNRDVVPLYFQARNSTFFYFLEKIREKMNS
                     RINFEMLFLVDEIFKQKGNTFTLYIGQPVSWKTFDESKTLQEWATVVKEMSYMLPEIT
                     N"
     gene            2296..2814
                     /locus_tag="PKH58_07595"
     CDS             2296..2814
                     /locus_tag="PKH58_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX88928.1"
                     /translation="MLAGFNIAYGQLASTTEDRQYLVKVGDMAPDFEMKLPEGGKVRL
                     SSLRGKVVMLQFTASWCGVCRKEMPHIEKEIWLRHKNNPRFALYGIDRDEPAATVRKF
                     AHDTGVTYPIGLDPKAGIFGLYALKEAGITRNVIIDENGKIVMLTRLYDEKEFAGMVT
                     LIDKLLSEMAQK"
     gene            2834..3304
                     /locus_tag="PKH58_07600"
     CDS             2834..3304
                     /locus_tag="PKH58_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNX88929.1"
                     /translation="MTYDQYEKIKKLEELKANGTLTEEEFQAEKEKVINGTSQTTATP
                     SQRQSLLGIDEKSYLLLMHLSQFISAFIIPLIMWLIGKEDSKNVDEHGKNIINFTISY
                     FIWGVIGVITVVIGIGIVILSVMGIAVTVFIIVAAVKAYNGEYWKYPLTISFLK"
     gene            3388..4017
                     /locus_tag="PKH58_07605"
     CDS             3388..4017
                     /locus_tag="PKH58_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNX88930.1"
                     /translation="MICTDIHTHRSEAYPIERTVRSLSLSEVRGMPLKPDIGLASVGI
                     HPWNVGSCYENDMEQLKVWIARDWVRLVGECGLDKYASAPYKLQLYFFRKQIELAEQF
                     CKPMIIHCVGYYNELFTLRKEMKPAQRWILHGFRGKPQLAEQALKAGCDLSFGEFFNK
                     DTVRITPVEHLFVETDESKLFIDDIYKMIAEIKDCKVEELNAGEKLLNC"
     gene            4128..5741
                     /locus_tag="PKH58_07610"
     CDS             4128..5741
                     /locus_tag="PKH58_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX88931.1"
                     /translation="MRKYSLLVLLFLTTNLFSQGISPVQQRKLVQALSAISSMYVDTI
                     NDTKIIESSIRGVLKDLDPHSVYTPKDEVQRMREPLEGSFDGIGVQFQILDDTIMVVQ
                     TIAGTPAEKVGVLPGDKIIYINDDLVAGSAAKVQNSDVFKRLRGKKGTEVLVKMKRGR
                     NPELIEFKIIRDKIPIYSVDASYMADSKTGYIKINSFGSTTYDEFTKALAGLKKEGMK
                     DLVLSLQGNGGGFLGTAIQIADEFLPANKLIVYTEGINQPRTDAKATVNGNYEKGRLI
                     VLVDEYSASASEILSGALQDWDRAVIVGVRTFGKGLVQREFSLVDGSMMRLTVARYYT
                     PTGRSIQKSYKNGTESYYKELADRYRNGELMHVDSIHFPDSLTYKTLVNARTVYGGGG
                     IMPDIFVPVDTTEYSDLHRSMVAKGVISRTAVEYLNSHREELKQMYPAFAKFKKQFET
                     GDDFLKLLLSEAEKEKIKYDDVQFNRSKRLIKLQLKALVARDLWNVNEYYQVLDSENE
                     SLQKALEILNTPGSYDRILTSSKLYTQQK"
     gene            5745..6365
                     /gene="pnuC"
                     /locus_tag="PKH58_07615"
     CDS             5745..6365
                     /gene="pnuC"
                     /locus_tag="PKH58_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNX88932.1"
                     /translation="MTEYLMANWVEITGAVLSVVYLILSIREKAGLWIFGFISSALYV
                     VVFFESKFYADMSLQFYYLAVSVYGWITWRRKTTATETGSLPVTLIPRKQYPGYVIAV
                     AVTYLIYFAVLKGWTDSPVPVLDSLVGALSVIATWMLARKKIENWLFWIVADALAAGL
                     FVYKGLYPTVVLYLIYTVMAVVGYFEWKKHLRIVTGKVEDILTKRN"
     gene            complement(6436..7080)
                     /locus_tag="PKH58_07620"
     CDS             complement(6436..7080)
                     /locus_tag="PKH58_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88933.1"
                     /translation="MEYDKSKFKIRNWKSAEMLHWIINPGLVFNEIILGQRLPKVMLI
                     EKKSSKTLQEKTKIPCPHCGTLHSGLKWSTKNNAFKNWFGLYCDNCGKTIPCLTNLTT
                     FLLLGLTFPVWIWFKDKWKMTWLKKQPERYQNMDLENVQNPFEEYGWIKLGLFWGLFM
                     YIFMVLLFPLIDGEGLSTWKVVLGIPIWTIGGIGFGYTMKLINGKNIKKQNQIE"
     gene            complement(7121..8329)
                     /locus_tag="PKH58_07625"
     CDS             complement(7121..8329)
                     /locus_tag="PKH58_07625"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNX88934.1"
                     /translation="MRKIITYILLTLFICVYAPAFSANKKVTSVPFEMAGSYVVLKIT
                     INDSSPLTMILDTGVKNTIINELMEDDILSLNNNVADSTLIQGLGNNRGIKAFRSYKN
                     DMKIGNITFTNKAVVMLPVNIFNFTNYTGKKINGILGSDVLRDYVVEINYTKSRVYFY
                     ESVNFVPPAKYSPISTTYANNKLYVNADYTGNDSIPRSVKMLVDTGAELGSWLFDSSI
                     SSLPEKKVYGYIGEGLSGEIMGYYARSVSLCLGPYCISNPIVTFPDTAYTKGLEQETP
                     RDGTLGNQTLKRFNCIIDFKKPAFYVKPNFMFKNKFRYNVAGIEVLREMPHFYITRVY
                     KVWKNSTAEAQGVKPGDLLLEVNGMKTFQMNVSEIRDIFETGRRFPLHIVIQRNDEVI
                     DLDLNMNSKI"
     gene            complement(8414..9058)
                     /locus_tag="PKH58_07630"
     CDS             complement(8414..9058)
                     /locus_tag="PKH58_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX88935.1"
                     /translation="MRKITTIILLCCAFLITCTDISAQNTDIELLKSFNQNESVLLRN
                     YSKVASNTTVYLVLAAPLTLGTISLIEKNDDLLKDAIYICSATALDGIITYSMKKAIH
                     RQRPYVSFPNEVTAYATENSFSMPSGHTSLAFTTATALSLSYPKWYVIAPGYIWACSI
                     GYSRMNLGVHYPSDVLAGAVLGAGSAFVTYKINQWYWKKREKKKLIGLENFENL"
     gene            9136..>9641
                     /locus_tag="PKH58_07635"
     CDS             9136..>9641
                     /locus_tag="PKH58_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX88936.1"
                     /translation="MIKIALTGPESTGKTELCIRLAEYFQAAYEPELAREYVEKLGYT
                     YTFEDVCTIARLQTEQEQRYAAGVENKPVFFDTDLIITKVWLEYKYGQVPAFVNEQLS
                     AHLIDLYLLCEPDLPWVSDPVREHGDDREFFFDWYEREINSLGTPYVKINGTGEKRFL
                     NALKAVED"
     assembly_gap    9642..9741
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9742..10506
                     /locus_tag="PKH58_07640"
                     /pseudo
     CDS             9742..10506
                     /locus_tag="PKH58_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
     gene            10862..11998
                     /locus_tag="PKH58_07645"
                     /pseudo
     CDS             10862..11998
                     /locus_tag="PKH58_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
BASE COUNT         3564 a         2438 c         2635 g         3435 t
ORIGIN      
        1 gcatgttttt gtggatcaca ccaattccac cttcacgggc aatggcaata gccatctttg
       61 cttccgtaac ggtatccatc gccgctgata cgataggtat cttcagttcg atgtttcggg
      121 taaaacgggt ggtgagatct acatcgcggg gcaatacttc agagtaggag ggaactagca
      181 acacatcatc gaaagtgaaa ccttcgaact gtatcttttc tgcaataaat gacatagtat
      241 gagtattttg gagttgtttt taatcgctat gaaaaatatt gcctgcaaat ttagtcattt
      301 tttcgcgggt tgtttcctgt ttcggtgtga aaataataca aattaacgta gtaatcagat
      361 tgaaatttat tctcatttac caaaacgtca ggcgcattca atcactttat tcggcagatg
      421 cttgtacaga aaggctgtaa atcgccatct gcggtaaaca tagattattt tttgtctttt
      481 acagagtgcc ttgaaaatta tacgtgcagc tttttcgggc ggacaaaccc agaacaccag
      541 tgtttttcct ttcatgtctg tttccatata tccgggtata atattggtaa ctgtaatcgg
      601 caatttcagg tagtgagcca gatttttcag tccttcaagg tagttcatct gaaaagcctt
      661 gctggcatta taggccggat tgttgcgcca gccacgcaaa ccggctaccg aagtgatgcc
      721 agccagatgt ccgtaaccct gcttgcggaa caaattgaat gcccagttgc acatcatgct
      781 gaaagcagta acattcacca tatttatttt gtattcaaca ttataatcaa gatcggtgtt
      841 caatttgatt aaaccggcgc tgatcaccag taaatccagt ccgcccaact catcaacaag
      901 ttcatccagt ttagtaagta caatatctgt ttccagcaaa tcgaatggtt tcacgacgta
      961 agcatccggt ttttcggcag ccagttcctg cagcagatat tcgcgacgag cggtaatgcc
     1021 cactttgtaa ctattctcag caagtgtttt agccaattct ctacccaccc ctgaagaagc
     1081 ccctacaaca atcgcctttt tcattcaaaa aaaatcattt tataagatta aacaacgaca
     1141 atcctgaaaa atccggtttg ttttgattat gcaaatttat gaatttttat tttcaaaaga
     1201 aattttagtt aatgaaacaa tacctgtttc gtaagtctaa cgctaaaaca ttgtattttt
     1261 gataaatgtt attcggaatt tggatgtttt tctgagcaat aatttttatt tttgtgtttt
     1321 cattgtaaaa atgacagatg aaggaagaaa taaaaaaagt tagaattgat tttgatcaac
     1381 ttgccagcac tctttttgca gggaaaaaga ttccccgctt attgatgaga attctgaaaa
     1441 aaatagcgca cgaagatgga ttaaacgaac tgtttgacag cgctccgggt aaaaagaata
     1501 tggattttgt ggatgcctgt atggactttt accggattga atgcagggtg aaagggatcg
     1561 aaaatctccc agccgatgca aaaccgctga tatttgccag taatcatccg ctgggcgctc
     1621 tcgacgcgat atcgatttta cattcgatag gtcaccgata cgatgggaaa attaagtttt
     1681 acgccaatga gatactgaac cacgttgagc ccctgaagga aatggtttta cccgtatcga
     1741 aatacagtta tcagggtaga aataatgtaa ggaaagttca ggaattcttc cgctcctccg
     1801 agcatttgct catttttcct gccggagcta cttcgcgcaa atacggggat aaaatcattg
     1861 acctggaatg gcagaaaaac tttattcaga aatcagtaca ttacaaccgg gatgtagtac
     1921 ccctctactt ccaggcccgg aattcaacgt tcttttattt tctcgaaaaa ataagagaga
     1981 aaatgaattc cagaattaat tttgaaatgc ttttcctggt tgatgagatt ttcaaacaaa
     2041 aagggaatac atttacattg tatataggtc agcctgttag ctggaaaaca tttgatgaat
     2101 caaaaacttt gcaggaatgg gcaactgtag taaaagaaat gtcgtatatg ctgccggaaa
     2161 taacaaattg acagtactaa cccattattt tatttgcatt ttctcaaaaa atacatacat
     2221 ttgtgatcaa attataaaat taaaatgaag gcagtttcaa aatttctttt tccggattta
     2281 accgcagtgt ttttaatgct tgcaggcttc aatattgcct acggacaact tgcttcaacc
     2341 accgaagacc gtcaatatct ggtaaaggta ggcgatatgg ctcccgactt tgagatgaaa
     2401 ctgcctgaag gaggaaaagt gaggttgtcg tcgctgcgcg gaaaagttgt catgctgcag
     2461 ttcaccgcca gctggtgcgg ggtttgccgc aaggaaatgc cgcatatcga aaaggaaatc
     2521 tggctgagac ataaaaacaa tccccggttt gcattgtacg gaatcgaccg cgatgaaccg
     2581 gcagccaccg tccggaaatt tgctcatgat accggagtaa cttatcctat cggactggac
     2641 ccgaaagccg gaattttcgg attgtatgca ctcaaagaag cggggattac ccgaaatgta
     2701 attatagatg aaaacggtaa aatagtcatg ctgacaaggt tgtacgatga aaaggaattt
     2761 gccggaatgg tgacattgat tgacaaattg ttatctgaaa tggctcaaaa ataatccttt
     2821 atattaaaca accatgactt acgaccagta cgaaaaaatc aaaaaacttg aagaactgaa
     2881 agcaaacggg acactcaccg aagaagaatt ccaggcggaa aaggaaaaag ttatcaacgg
     2941 aaccagccag acgactgcaa caccctctca acgccagtca ttactcggaa tagatgaaaa
     3001 atcatatctg ttactcatgc atttatcaca gtttatctct gcctttatca ttcccctcat
     3061 catgtggctg attgggaagg aagacagcaa aaatgtggat gaacatggta agaacatcat
     3121 caatttcacc atttcctatt ttatctgggg agtaattggc gttataacgg ttgtcattgg
     3181 tatcggaatt gtcattttat cggtgatggg tattgccgtt actgtgttta ttatcgtagc
     3241 ggcagtgaaa gcctacaacg gagagtactg gaaatatccc cttaccatca gtttcctgaa
     3301 ataaatcagt taaaaacaaa tatataacgt gtggagtaga aattacttca cacgtttacc
     3361 gttttataaa attacaattt taccattttg atctgtacag atattcatac acataggtct
     3421 gaagcatatc cgatcgaacg cacggtgagg agtttgtcac tctctgaggt tcgtggcatg
     3481 ccactgaaac cggatattgg cttggcatcg gtgggtattc atccatggaa tgttggttcg
     3541 tgttatgaaa atgatatgga acaactgaaa gtctggattg cccgggactg ggtacgactg
     3601 gttggtgagt gcgggcttga caaatacgcg tctgctcctt acaagctgca actttatttt
     3661 ttcagaaaac aaatcgaact cgccgagcaa ttctgcaaac ccatgattat ccactgtgtg
     3721 ggttattaca acgaattatt cactttacgt aaggaaatga aacccgctca aaggtggata
     3781 ctgcacggat ttcggggaaa accccaactg gcagaacaag cgttgaaagc cggatgtgat
     3841 ttgtcgttcg gcgaattctt taacaaggac accgtcagaa ttactccggt ggaacatctc
     3901 tttgtagaaa cggatgaaag caaattgttt attgatgata tctacaaaat gatagctgaa
     3961 attaaggatt gtaaggtgga agaattaaac gccggtgaga aattacttaa ctgttaaata
     4021 taattttcta acattttatt tctgtaaaag tgttgaatga taacatgcta attatgtagc
     4081 tttgtctctg tttttttgag taaatttatt ttatgattta tcacacaatg agaaaataca
     4141 gcctgcttgt acttttattt ttaacaacca accttttttc acagggaatt tcacccgtac
     4201 aacaacgcaa acttgttcag gctctttcgg ccatttcaag tatgtatgtg gacaccatca
     4261 acgatacgaa aataatcgaa tcgtccatcc gtggtgtgct gaaagatttg gacccgcatt
     4321 cagtttatac acccaaagat gaggtacagc gtatgcgcga gccgctcgaa ggaagctttg
     4381 atggcatcgg cgtacagttt cagatactgg acgataccat catggtggtt caaaccatag
     4441 ccggaactcc tgccgaaaaa gttggtgtgc tgcccggcga caaaatcatt tatatcaacg
     4501 atgatctggt agcaggttcg gctgccaagg tgcagaattc ggatgtgttc aaacgactgc
     4561 gcgggaaaaa gggtacagaa gtgttggtga aaatgaagcg gggccgaaat ccggagctga
     4621 tcgagttcaa gataatacgc gataagattc caatttacag cgtggatgcc tcgtatatgg
     4681 ccgacagtaa aacgggttac atcaaaataa acagttttgg gagcactacg tatgacgaat
     4741 ttactaaagc gctcgccggt ctgaaaaaag agggaatgaa agatcttgtc ctgagcctgc
     4801 agggcaatgg gggaggattt ctgggtacag ccattcagat tgctgatgag ttcctgcctg
     4861 caaacaaact cattgtatat accgaaggaa tcaaccagcc acgcacagac gcgaaggcta
     4921 ctgtcaatgg caactacgaa aaaggcagac tgattgttct ggtggatgag tattctgcat
     4981 cagccagcga aattctgagc ggagcattgc aggactggga cagagcggtg attgtcggtg
     5041 tccggacgtt tggcaaggga ctcgttcagc gcgaattttc gctggtggac gggtcaatga
     5101 tgcgacttac ggtggcaaga tattacacgc ctaccggccg cagtattcaa aaatcttaca
     5161 agaacggtac cgaaagttat tacaaagaac tcgctgaccg ttacagaaac ggtgagttaa
     5221 tgcacgtaga cagtattcat ttccccgact cgctgactta caaaaccctg gttaacgctc
     5281 gcacggtgta cgggggaggt ggtattatgc ccgatatttt tgtacccgta gatacaacgg
     5341 agtactccga tctgcaccgg tcgatggtgg caaagggcgt gatcagccgt acagcggtgg
     5401 aatacctgaa cagtcaccgt gaggaactga aacaaatgta tcccgcgttt gcgaaattca
     5461 aaaaacaatt cgaaaccggt gacgattttc tgaaactact gctctctgaa gctgaaaaag
     5521 aaaaaataaa gtacgatgat gttcagttta accgctccaa acgactgatt aaactgcaat
     5581 tgaaagctct ggttgcccgt gacttgtgga atgtaaatga atattatcag gtgctggaca
     5641 gtgaaaacga atcgctgcag aaagcgctcg aaatactaaa cactccgggt tcgtacgacc
     5701 ggatacttac ctcttcaaaa ctgtacaccc aacagaaata ggtgatgacg gagtatctga
     5761 tggcgaactg ggtggaaata accggagcag ttctgagtgt ggtgtacctc attctttcca
     5821 ttcgcgaaaa agctggattg tggatattcg gctttatcag ttcagcattg tatgtggtcg
     5881 ttttctttga gtctaagttt tatgccgata tgtcgctcca gttctactac ctggctgtga
     5941 gtgtttacgg ctggataacc tggcgccgga aaacaacagc gacagaaaca ggtagtcttc
     6001 ctgttactct cattccccga aaacaatatc ccggttacgt catagctgta gctgtaactt
     6061 atctcattta cttcgctgtt ctgaaaggat ggaccgattc tccggttccc gtactcgact
     6121 cgctggtggg agctttaagc gtgattgcga cctggatgct tgcccgtaaa aaaattgaaa
     6181 actggctgtt ttggattgtt gccgatgcac tggctgccgg attatttgtt tacaaaggct
     6241 tgtatcctac cgtcgtgttg tatcttattt acactgtaat ggctgtggtt ggttattttg
     6301 aatggaaaaa acatctccgc atagtaacgg gtaaagttga ggatatatta acaaaacgga
     6361 attagaagta cttattactt aagtgattaa aaactacgaa catcaacaat ggtaaaataa
     6421 cagttgcaaa cctggttatt caatttgatt ctgttttttt atattttttc cgtttattag
     6481 tttcatcgta taaccaaaac cgataccgcc gatcgtccag ataggaatac ctaacacaac
     6541 tttccaggtt gataatcctt ctccgtcaat cagcgggaac aagagaacca taaaaatgta
     6601 cataaataat ccccaaaaaa gtcccagttt tatccagcca tattcttcaa acgggttctg
     6661 cacattttct aaatccatat tttgatatct ttcaggttgt tttttcagcc atgtcatctt
     6721 ccacttgtcc ttaaaccaaa tccaaaccgg aaaggtgagg ccaagcagca ggaaagtggt
     6781 cagatttgtc agacagggaa tagtttttcc acaattgtcg caataaagcc cgaaccaatt
     6841 tttgaaagcg ttattttttg tcgaccattt aagtcccgaa tgtagcgttc cacaatgcgg
     6901 gcaaggtatt tttgtttttt cctgtaatgt cttcgaagac tttttttcaa tcagcataac
     6961 ctttggtagc cgttgcccta aaattatttc gttgaatacc agtcccgggt ttattatcca
     7021 gtgcaacatc tcagcgctct tccagttccg gattttgaat tttgatttgt cgtattccac
     7081 tttctttatt ataattcgta ttatcgttca ataacacact tcagattttt gaattcatgt
     7141 tcaggtccag gtctatcact tcatcgttcc gctgtatgac tatatgtagc ggaaaccgtc
     7201 tgcccgtctc gaatatatcc ctgatttcgg atacattcat ctgaaaggtt ttcatcccat
     7261 ttacttccag caacaggtca cccggcttta cgccctgtgc ttccgcggtt gaatttttcc
     7321 agactttata gacccttgtg atgtaaaaat gaggcatttc gcgtaggact tcaatcccag
     7381 ccacgttgta gcggaatttg tttttaaaca tgaagttggg cttcacatag aatgccggtt
     7441 ttttgaaatc gatgatgcag ttgaagcgtt tcagtgtctg attacccagc gtaccatcgc
     7501 ggggagtttc ctgttccagt cctttggtat aggcggtgtc cggaaaggtt actatcggat
     7561 tgctgatgca ataagggccg aggcataaac tcaccgatcg ggcatagtaa cccatgattt
     7621 caccactcag cccttcgccg atataaccgt acactttttt ctcgggcaac gaactgatac
     7681 tgctgtcaaa cagccaagaa ccaagttctg cgccggtatc cacaagcatt tttacgcttc
     7741 gcggaatgct gtcatttccc gtataatcag cattgacata caatttgttg ttggcataag
     7801 tagttgaaat tggtgaatac tttgcaggtg gaacgaaatt tacactttcg taaaaataca
     7861 cgcgtgattt ggtgtagttt atttcaacta cataatcgcg taacacgtca gagccaagta
     7921 ttccgtttat ttttttacct gtatagttgg tgaaattgaa tatatttaca ggcagcatta
     7981 ccactgcttt attggtgaat gtaatattcc ctattttcat atcgtttttg tagctccgga
     8041 aagccttaat cccacgattg ttgccaagtc cctgtatcag cgttgaatcg gcaacattgt
     8101 tgtttagtga aagtatgtca tcttccatca gttcgttgat aatggtgttt ttgaccccgg
     8161 tgtcgagtat cattgtgagg ggcgacgagt cgttgatggt aatttttaac actacatagc
     8221 tgcccgccat ttcgaacgga accgatgtca cctttttatt tgctgaaaat gcgggagcat
     8281 aaacgcagat aaaaagggtg agcagtatgt atgttataat ctttctcatg ccgtttgtaa
     8341 tgtacggatt ggtaaattat acttcaattc attaatgact tttttgtatt gagtaattac
     8401 ctatttaacg taattataaa ttttcaaagt tttccaaacc gatcagtttt tttttctccc
     8461 gctttttcca ataccactga ttgattttgt aggttacaaa cgcgcttccg gcacccagca
     8521 cggccccggc aagcacatcg ctagggtaat gtacccccag attcatcctc gaatatccaa
     8581 tagagcaggc ccagatatat cccggcgcaa tcacatacca tttcggatag ctgaggctga
     8641 gagctgttgc tgttgtaaat gccagtgaag tatgtcccga aggcatcgaa aatgaattct
     8701 cggtagcgta ggctgttacc tcgttaggaa aactaacgta cggtctctga cggtgaattg
     8761 cttttttcat cgaataagta ataattccat ccaaagctgt cgcactgcaa atataaatgg
     8821 catcttttaa taagtcatca tttttttcaa tcaatgagat ggtgcccagt gtcaagggag
     8881 cagccagaac aagataaaca gttgtattgg atgcaacctt agaataatta cgaagcagca
     8941 ctgattcgtt ttggttaaaa cttttcagaa gctcaatatc tgtgttttga gccgaaatat
     9001 ctgtgcatgt aatcaaaaaa gcacaacaaa gcagaataat tgttgtaatt ttgcgcattg
     9061 cttaagggga tgattttaaa accattgcaa atataacgta aattctcaat aatcagtcat
     9121 ctgttgtagg ttaccatgat aaaaatcgcg ctgacagggc ctgagtcgac gggtaaaacc
     9181 gaattgtgta ttcgtctggc tgaatatttt caagccgcgt atgaaccgga gcttgcacgc
     9241 gaatatgtcg aaaaacttgg atacacctat acctttgagg atgtatgcac cattgcccgc
     9301 ctgcaaaccg aacaggaaca acgatatgct gccggtgtag agaataaacc ggttttcttt
     9361 gacacagacc tgattattac caaagtgtgg cttgagtata aatacgggca ggtacctgca
     9421 tttgttaacg aacagttgtc cgctcatctg atcgatttgt atctgctttg tgaacccgat
     9481 ttaccctggg tgtccgatcc ggtcagggaa catggcgacg accgcgaatt cttttttgac
     9541 tggtacgaac gggaaatcaa cagcttggga acaccttatg tcaaaataaa cggtacaggc
     9601 gaaaaacgtt tcctgaatgc gctgaaggcc gtcgaggact tnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn ncggattgta cagttgtgcc gggctgttct ttttccggga
     9781 tattttggca attcggatgt gaataagaat aacgttgcac agcacatcga acaaaacatc
     9841 attctgttgc atcagttgtt aacccagcag attaaatccg ggatgtgctt cgataattca
     9901 acgatcgatt atacctgcaa gaaactcgaa gcgcagtcca tcgctatcgc tgccgatttt
     9961 gtaagtcagt tgcctgtgat ccgtgaaata ctccacaccg atgtaattgc agctttcgat
    10021 ggagatcctg ctgctaaaag ttacggcgaa gttatttttt gttatccggg catacgtgcc
    10081 atcagcagct atcgcattgc tcacgccttg ctgaagttgg gcgttcctgt aattccgcgt
    10141 atgattaccg aaaaagctca ttcagagaca ggtatcgata ttcatcccgg agcagttatc
    10201 ggcgactatt ttaccattga tcacggaaca ggtgtggtaa tcggcgaaac agctgtcatt
    10261 ggcagtaatg tgaaaatgta tcagggagtt actctcggag cgaaaagttt tccgcttgat
    10321 gaaaacggca atcccatcaa aggcatcgac cgccatccga aaattgggaa taatgttatc
    10381 atttatgcta attccaccat tctgggaaat atcaaagttg gcgatgactc agttatcggt
    10441 ggaaatatct gggtagatac ggatgtgcct cctggaaccc ggttgttaca acgcaaacgt
    10501 gaagaatacc acgattcacc ctccggcaat gaaaaaaaaa agaaaaaaca caaagaagcc
    10561 aaagtctatg gggagatatt aaaggaaccc aagtcgaaga ataaagataa gaaagatgaa
    10621 aagcgtaaca aaaagataaa ttatacgcca atgaaggcgg gcaacagtta tgcaatgatt
    10681 tttcagaagt gcaaaggttg caaaagcctg aatggttgct ccaaatgccg cgctaaatgg
    10741 gaaaagaaac aaggaaagat tattgagtga aaagtttgtc aagttttgac ttaataaact
    10801 ttataaactt tccagcttga tgaactagac aaacttgtaa atgataaact aaaaaaggaa
    10861 tatggaattt gaaatgatag cgaagacctt taagggtctg gaagaagtgc ttgctactga
    10921 acttgtggca atgggtgcca ataatgtaca gcttcaaaga cgcgcagtaa gttttaccgg
    10981 caataaggaa ctaatgtaca aagccaatat gcaattacga acagcatcaa gggtactcaa
    11041 acccatcgct gccttcaagg cgctaaatcc cgatgaagtt tatgagcagg taaaaaagat
    11101 tgactgggag aaatatatga gtgtggatac tacttttgcg atcgattcca ctgttttttc
    11161 ggatgagttt cgtcattcca tgtttgttgc ataccgggtg aaagatgcta ttgccgaccg
    11221 gtttatggaa aaacagaata aacgaccctc agtgagactg gataatccgc aactgatgat
    11281 taatatacat atcgctcaaa atcagtgcac catttccctc gatagttcag gagaatcact
    11341 gcacaagcga ggttatcggg tggcacaaac cgatgcaccg ctcaacgaag ccctggctgc
    11401 cggtatgctg ctgatgagcg gttgggaagg taaaaccgat ttcatcgatc cgatgtgcgg
    11461 atcaggtact ttgctgatag aagcagcatt gattgccctg aatatcccgc cgggcgtatt
    11521 ccgcagggaa tttgcctttg aaaaatggtc cgatttcgac agcgagttgt ttgatgcggt
    11581 ttacaatgac gacagcgttg aaagaccgtt cgaacacaaa atctatggct cggatatctc
    11641 tcccctagct atcaaaatag ccgacaaaaa tgtgcggagc gctggcctca acaagtacat
    11701 cgaactgaag gtgttgcctg tgcagcaact cgaagctccg tcagatcatt gcatgatggt
    11761 gacaaatccg ccctacggag aacgaatcac ttcgcccgat attttcggat tgtacgcgtc
    11821 gcttggaact gtgttgaaac ataaatttgc cggaagttcc gcctgggtga tcagttcgca
    11881 cgaagaatgt ctggacaaaa tagggttgaa accctcgaag aaagttcagc ttttaaatgg
    11941 ttcgctagat tgcctttact gtcagtacga aattttcgaa ggaaaacgta acgattttgt
    12001 tgccgataag aagaaaaggt attgagttat taaggcggca gtatttcctg aaaataattt
    12061 caggaaatta caaaaatctt tgccagtttc aaaaaatagt gtacttttgt agccgctaat
    12121 cgcccagatg gcggaattgg tagacgcgct ggtctcaaac accagtggat tc
//
LOCUS       DAOPYB010000083        12136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_5884_length_12136_cov_8.820296, whole genome shotgun sequence.
ACCESSION   DAOPYB010000083 DAOPYB010000000
VERSION     DAOPYB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5884_length_12136_cov_8.820296"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..3680)
                     /locus_tag="PKH58_07650"
     CDS             complement(9..3680)
                     /locus_tag="PKH58_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX88937.1"
                     /translation="MKKIFILFAAFVTLLTGLQAADLTGVKIYLNPGHGGYDGANDRN
                     VETIPYALGDTLGFWESWSNLRKALALRNRLESAGATVYMSRTQNRDEDDRLLSEIAE
                     EANANDVDAFLSIHSNALGSNAGTNYLLFLFHGYDADPTVPESKTQATAAWPFLFENN
                     LTWWSNYTTTTNIRGDFSFYGNTSGLGVLRPLVVPGFLSEGSFHDYKPETHRLLNEAY
                     RKLEADRFYKYFCNYFGADMPATGTIAGWVKGIDQRVNDTRYIYKAGTTDEWLPLNGA
                     KVKLMNAAGDSLNSYTVDNNYNGVYAFFNLTPGTYKLRFSAFSHQIKDTTVTVTAANT
                     TYARMMMYNPNLPLYKIVPPDYPNPVQAAGTIPMNHYSFQSLTQTNPDWLNGATVKKV
                     LFRNEKLYVLTTEPKILIVSASTFQQIGEINLTGITGGTEILSDIQFTSNGHLLACNK
                     VTLANPETGGNYFKVYSWETDGSIPLLYFQTQLQGNMPSAVVGETFAVTGAFWNSSVY
                     ASSVNSTDNSLKIVAYHVEDSMIVSSKYMTAPGLIGNTTADRLNFTISPDSTSYLIID
                     RASALPAEYSFDWNAADDSPVSLRKNFSEQNGYLLQPAATGNYYFRHARHLYMAAPNC
                     NADATAVGVVLFDITAGLDQARKVSEFLPEGGLGTASSPCMAAAAKVTDYDIELIIVA
                     EKEGAARFKTTSIPTPAVYASELKSTPIANGYRLTFTLNGNIQSGKITVLNGTTEIYT
                     VPLGALSKGTQSVDLVTVDIPAGTYSWDVTVNGNAIDRPYKLSDNASALMQFNYPNGV
                     AVDNNPASSYFGRIYVSESAGGALTARTTQDGVYILDAALTDITTQGATAYNGSVAWG
                     ATSSSSPMRLSVGDDGTVFVCDWSDAHPGVWMMNPASPTAAFKPVFEGLTKATSGLSS
                     FNGVNIHGSISHCYSMGTGENRRLYTFDEDYINETATSPGNMLQYNIGTLAAPYQTAP
                     LVVYNDAANGNLQQNGNSCIAPDSRGGWWISQYRATDAALIPSLIHVNTGGTVDYNSG
                     LTPTFIENSYRAGMAVSYDGKLVAMGCNNEIKILGITYSGSGIPSLKLLYSIKPALGM
                     NTTGLSFDRAGNVYAVSYTDKRLGVWSLPKIDNTFRTPAPLTQQIVVTSTGTNEIGKD
                     SQSIRFFPNPVSASLTIQSANAPIETISIYELSGRLLIQTEGLGTEQHFKLNELSSGV
                     YIIKIKTGKESFTGRIIRK"
     gene            complement(3694..4599)
                     /locus_tag="PKH58_07655"
     CDS             complement(3694..4599)
                     /locus_tag="PKH58_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88938.1"
                     /translation="MKKLLLLFILLSQFAFSQQVNVISTEKLQTTNSGGYFYPRISPD
                     NQFILMTRGNYSGLYKYSLNDKTLKTLNEDPGAGYKVQISEDGNTVLYTKSELIRKLR
                     HNSLISQTIATGQKKVLVTPTRELITARLVNSKPVYVTKRSMIKSASLKLTDNLYVIT
                     IENRKMVVYKNGVRNELTPNGTDCSYIWASISPDNKNIVYTATGKGTFVCSVTGNNVV
                     SLGKLSAPSWAGNKYVVGMDDRDDGEKLISSSIIISSIDGKLRKRLDTPSGINAMYPS
                     ASKDGSLITFNTDRGEVYLMRVELK"
     gene            4965..6413
                     /locus_tag="PKH58_07660"
     CDS             4965..6413
                     /locus_tag="PKH58_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNX88939.1"
                     /translation="MNMKTLEPTIVWNYFYEITQIPRPSKKEDKVRAYLEDFGKKNNL
                     PTVIDKAGNVLITKPATPGYEDGKTLILQAHMDMVCEKNSDVEHNFETDPIQIIEDNG
                     WIKAKGTTLGADNGIGMAYMLAVLASDNLQHPAVECLFTTDEETGLTGAFALDDNMLK
                     GDILINLDSEDDGEIFVGCAGGIDTTATLKYKIKDTPRHFFAFSVTVKGLLGGHSGDD
                     IDKRRANANKLLNRILWNMHKEMDLHLVKIDGGNLRNAIPREASAVACVPYADKEKVR
                     VIFNQVAAELEDENSAVETGMRIELHSEELPARTMTRRSTARILNAIYACPHGIKRMS
                     ADMPGLVETSTNLASVKMQEDKKLLITTSQRSSVESAKYDIAHQVESVLTLAGCEVTH
                     GDGYPGWKPNLESQILKVAEKSYKKLFDVQPKVKAIHAGLECGLFLKKYPHLDMISIG
                     PTMRGVHSPDERLEIGTVARVWSWLTDILATK"
     gene            6519..7751
                     /locus_tag="PKH58_07665"
     CDS             6519..7751
                     /locus_tag="PKH58_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX88940.1"
                     /translation="MKKQNKRKIINDPVFGFINVPNDFLYDIIQHPYFQRLNRIRQLG
                     LSSFVYPGAQHTRMLHSLGAMHLMGEAIHQLREAGQEINAHEAEAVRACILLHDIGHG
                     PFSHTLENSIVRDIAHEQISLWMMQRMNDEFGGKLTLALDIFTNNYHKHFLHQLVSSQ
                     LDMDRLDYLSRDSFYCGVSEGIIGAARIIKMLNVHNDKLVVEAKGIYSIEKFLVARRL
                     MYWQVYLHKTSIAAEIMLISILRRAKELALKGEVLFGPPSLLYFLSGDITLNHFESSP
                     ETLDHFAQLDDSDILSAVKVWSSHPDKVLSTLCHDFSNRKLFKVEILTEKEKGEILRK
                     HYLEKYQRHFNISEQEAGYFLGVETVSTDTYSPKDDTIDILLKDGTVKDIAEASDMLN
                     IQVLTKKVEKHFFCYCRL"
     gene            7890..8933
                     /gene="lpxD"
                     /locus_tag="PKH58_07670"
     CDS             7890..8933
                     /gene="lpxD"
                     /locus_tag="PKH58_07670"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNX88941.1"
                     /translation="MEFSAQQIASFLNGEIVGNPTVTVSNFSKIEEGKPGTLSFLSNP
                     KYSHYIYDCNSDIVLVNRSFVPERDVKATLIKVENAYESLAVLMGIVEEYKPKKNGVS
                     SLAYIAPTAKIGANVYVAPYVYVGDNAEIGNYSTLDSFTYVGDGAKVGNNCQLHAGVK
                     IEKECIIGNSCILHAGVVIGSDGFGFAPTDDGSYRKIPQTGNVIIEDNVEIGANSTID
                     RATLGSTIIHKGVKIDNLVQIAHNVEIDENTVIAAQSGVAGSTRLGKQCVLAGQVGIA
                     GHLEIADGSVFGAQTGVPGSLKTPNQIYQGYPAVPVATFRRASVVYKNLPELQKTVYE
                     LQKQLAELQSKIK"
     gene            9025..>9269
                     /locus_tag="PKH58_07675"
     CDS             9025..>9269
                     /locus_tag="PKH58_07675"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNX88942.1"
                     /translation="MATQKTLKSSFELKGKGLHTGLEIEIKFHPAAPSTGIRIKRTDM
                     EAKPEISALADYVTDTNRGTVLKRGEFQVSTIEHAMAA"
     assembly_gap    9270..9369
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9370..10257
                     /gene="fabZ"
                     /locus_tag="PKH58_07680"
     CDS             <9370..10257
                     /gene="fabZ"
                     /locus_tag="PKH58_07680"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNX88943.1"
                     /translation="ILSNQFASIDSLEEFEKEIAGARTFVFVREIEPLLKQNLIKGGD
                     LKNAIVIYDRRMEQEEFDKLADMMNQPHLKVGEFGIISGDLKFDNEPARHKLLDVIGD
                     LALTGKHIQGKVIANYPGHKVNTELAKYIRKEIKKQEVQAPVYDETVPPVLDTVAIRK
                     MLPHRWPFLMVDKIIEIRERSIIGVKNITVNETLFMGHFPDEPVMPGVLLVEAMAQTG
                     GLLMLSQLDEPERYSTYFMKIDNVKFRQKVVPGDTVIFHVSLIDDIRRGIATMRGYAF
                     VGGVITTEAEFMAQIVKNK"
     gene            10365..11144
                     /gene="lpxA"
                     /locus_tag="PKH58_07685"
     CDS             10365..11144
                     /gene="lpxA"
                     /locus_tag="PKH58_07685"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNX88944.1"
                     /translation="MKQPLAYIHPDAKIAPTVVIEPFVTIDKNVVIGEGTRIGSNVTI
                     LEGVRIGKNCTIFPGAVIGAVPQDLKFNGEDSLAIIGDNTTLREFVTVNRGTASKGKT
                     VVGSNCLLMAYCHVAHDCVIGDHVIMSNSCQLAGEVEIDDYAVLGGGTMIHQFSRIGA
                     HTMIQGGSKVTKDVPPFITAGRDPLSYAGVNSIGLRRRGYTNEQIRDIQDVYRFIFQS
                     GMNISHSVERVQAELSATKERDEILLFIKNSPRGILKGYFD"
     gene            11193..>12136
                     /gene="glnS"
                     /locus_tag="PKH58_07690"
     CDS             11193..>12136
                     /gene="glnS"
                     /locus_tag="PKH58_07690"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNX88945.1"
                     /translation="MTTDNNAEETPKRSLNFIEQIVEEDLSAGKNNGRIQTRFPPEPN
                     GYLHIGHAKAICMDFGIARDYNGICNLRFDDTNPVKEDVEYVDSIMEDIKWLGYEWEN
                     VYYASDYFQQLWDLAVKLIKDGKAYVDEQSAETIAKQKGTPTEPGTESPYRNRPVEEN
                     LALFEKMNTGEIPEGAMVLRARIDMASPNMHFRDPIMYRVISSHPHHRTGFTWKAYPM
                     YDYAHGQSDYFEGVTHSICTLEFVVHRPLYDWFIDQFQDTDYRPRQIEFNRLNITYTV
                     MSKRKMLQLVQEGLVSGWDDPRMPTLCGLRRRGFTPQS"
BASE COUNT         3436 a         2441 c         2748 g         3411 t
ORIGIN      
        1 ctgattgttt atttcctgat aattcttccg gtaaagcttt cttttccggt ttttatcttg
       61 atgatatata cgcctgaact tagttcgttc agtttaaaat gttgttcggt tcccagtcct
      121 tcagtctgga ttagcagtct cccgctcaat tcataaatac tgatggtttc aatcggagca
      181 tttgctgatt gaatggtcag gctggccgaa acggggttgg ggaagaagcg gatggattga
      241 ctgtccttac cgatttcgtt tgtgcctgta ctcgtaacca ctatctgctg tgtcaatggt
      301 gccggtgtcc ggaaagtatt atctattttc ggcaatgacc atacacccaa tcgtttgtcg
      361 gtgtatgaaa cagcatacac gttacctgca cggtcgaacg ataatcctgt tgtattcatt
      421 cccaaagccg gttttatcga ataaagtaat ttgagtgacg gaatacctga gccggagtaa
      481 gttatcccga gaatttttat ttcattgtta cagcccattg ctacaagttt tccatcataa
      541 cttacagcca ttccggcacg atatgaattt tcaataaacg taggggtaag tccggaattg
      601 taatccaccg tacctccggt atttacatgt atgagcgaag gtataagtgc tgcatcggtt
      661 gcccggtatt gggatatcca ccatcctccc cggctgtcgg gtgcaatgca ggaattgccg
      721 ttttgttgca ggttgccgtt tgctgcatcg ttgtaaacaa ctaaaggtgc tgtctgatag
      781 ggagctgcaa gtgttccgat gttgtactga agcatatttc cgggactggt tgccgtttcg
      841 tttatgtaat cttcatcgaa agtatataat cgtctgtttt ctcccgttcc cattgaatag
      901 caatgtgaga tggagccgtg aatatttact ccgttaaacg aagataaacc ggaagttgct
      961 tttgtgagcc cttcaaatac aggtttgaac gcagctgtgg gagaagccgg gttcatcatc
     1021 catactcccg gatgtgcatc gctccagtcg catacaaaaa cagtaccgtc atcgcctacc
     1081 gacaaacgca tgggactgct gctgcttgtt gctccccagg ctacactgcc gttgtaagca
     1141 gttgctccct gagtggttat gtctgtaagt gctgcatcga gaatgtatac tccatcctga
     1201 gtggtacggg ccgttaatgc tccgcctgcc gattccgata cataaattcg cccgaaatag
     1261 gaacttgccg gattattatc tacagcaacc ccgttgggat agttgaactg cataagtgca
     1321 ctggcattat ccgaaagttt atacgggcgg tctattgcat tcccattcac tgtaacatcc
     1381 cagctatagg tgcctgctgg aatatcaacg gtaacgagat cgacactttg agttcctttc
     1441 gaaagtgctc ccaggggaac ggtataaatt tctgtcgttc cgttgagcac ggtaattttt
     1501 ccgctctgaa tgttaccgtt cagggtaaag gtgagtcgat agccgttagc aatcggtgtt
     1561 gactttaatt ccgaagcata tacagcaggt gttggaatag aggtggtttt gaaccgtgca
     1621 gctccctctt tctcggcaac gataatcagt tcaatatcgt aatcggtaac ttttgccgca
     1681 gcagccatac agggcgaaga agctgtgccc aatcctcctt ccggcagaaa ttctgaaact
     1741 ttcctagcct gatctagtcc cgcggtaatg tcgaacaaca ccactccaac tgccgtagca
     1801 tcggcattac agttgggtgc agccatatat agatgccggg catgccggaa gtaataattg
     1861 cctgtagctg cgggctgtag taaatagccg ttttgctcgc taaagttttt ccgcaaactt
     1921 accgggctat catcagcagc attccagtcg aaactgtatt cggcaggcaa tgcgctagcg
     1981 cggtcaataa taagataaga tgtggaatcg ggagaaatgg tgaagttcag acgatcggct
     2041 gtcgtgttgc caatcaaccc gggtgcagtc atatatttcg aactgactat catactatct
     2101 tccacatgat aagccacaat cttcagcgaa ttgtccgttg agtttaccga ggaggcatat
     2161 acactcgaat tccagaatgc acctgtaact gcaaatgttt cacctaccac agccgatggc
     2221 atattaccct gaagctgtgt ctggaaatag agcaacggaa tactgccgtc tgtttcccac
     2281 gaatatactt tgaagtagtt accgcctgtt tcgggattgg caagagttac tttgttgcat
     2341 gcaagtaaat gtccgttaga ggtgaattga atgtcactca atatctctgt acctcctgtt
     2401 attccggtta ggtttatttc accgatttgc tgaaaggttg aagcgcttac aatcagaatt
     2461 tttggttcag tggtaagaac atataatttt tcgttacgga atagtacttt ctttaccgtt
     2521 gctccgttca gccagtcggg attagtttgc gtcaacgact gaaaactgta atggttcatc
     2581 ggaattgttc ctgcagcctg tacgggattg ggataatcgg gaggaacaat tttatacagt
     2641 ggcagattcg gattgtacat catcatccgg gcgtacgtag tatttgcagc ggttacagtt
     2701 acagtggtgt cttttatctg atgactgaaa gcagagaacc gcagtttata ggttcccgga
     2761 gtcaagttga agaatgcata cactccgttg tagttattat ccacagtgta actgttgagt
     2821 gagtctcctg cagcattcat cagtttcact tttgcaccgt tgaggggtag ccattcatct
     2881 gtcgttccgg ctttgtaaat gtaccgggta tcattcaccc gctgatcgat tccttttacc
     2941 caacctgcta tagtaccggt tgcaggcata tcagctccga aataattaca aaagtattta
     3001 tagaaacggt cggcttcgag ttttcggtaa gcctcattta gcagtctgtg agtttcgggt
     3061 ttgtaatcgt gaaatgatcc ttcggacaag aagcccggaa caacaagagg ccggagtact
     3121 cccaagccgg atgtgttccc gtaaaacgag aaatcacccc ggatattagt tgttgtggtg
     3181 taattgctcc accaggtaag attgttttcg aataagaaag gccatgcggc tgtagcctgt
     3241 gttttgcttt cgggtaccgt aggatcagcg tcgtatccgt gaaacaggaa tagcaggtaa
     3301 ttcgtaccgg cgttactgcc cagtgcattg ctgtgtatgg aaagaaaagc atccacatcg
     3361 ttggcattgg cttcttcggc tatttcagat aaaaggcggt catcctcatc acggttttgg
     3421 gtacgtgaca tatatacggt tgctccggca gattcaagtc tgtttcttaa tgccagcgct
     3481 ttgcggagat tgctccacga ttcccagaag cccagggtat cgccaagggc ataaggaatt
     3541 gtttccacgt tccggtcatt ggctccgtca taacctccat gtcccggatt gaggtaaatt
     3601 tttactccgg taagatcggc ggcttgcagt cctgtcaata gagtaacgaa agctgcaaaa
     3661 aggataaata tctttttcat attcgctcct ccgttattta agttcaacac gcatcagata
     3721 cacttctccg cggtctgtat tgaatgttat caagcttcca tctttcgaag ctgacggata
     3781 catcgcgtta atgcctgatg gagtatcaag cctttttcga agttttccat ctatggaaga
     3841 gataatgatt gatgaagaaa tgagtttttc gccatcgtcg cggtcatcca tgcctaccac
     3901 atatttattg cctgcccacg agggagcgct gagtttgccc agcgaaacca cgttattacc
     3961 tgtaacggaa catacaaagg taccttttcc ggttgctgtg taaacgatat ttttgttgtc
     4021 gggtgaaatg gaagcccaga tgtagctgca gtcagttcca ttgggagtca gttcatttcg
     4081 tacaccgttt ttgtacacca ccattttccg gttttcgatg gttatgacat acaggttatc
     4141 tgtaagtttt aatgatgcgg acttaatcat acttcgtttt gtaacataca ctggtttgga
     4201 attaaccagt ctcgcggtta tcagttcgcg ggtgggggtg actaaaacct ttttctgtcc
     4261 ggtggcaata gtctggctta tgagcgaatt gtggcggagt ttacggataa gttccgattt
     4321 ggtatacagt acggtgttgc catcttcact gatctggact ttatatcctg cccccggatc
     4381 ttcattcagg gttttcagtg ttttatcgtt caatgaatac ttgtacaaac cgctgtaatt
     4441 gccacgggtc atcaaaataa actgattgtc gggactaatg cggggataga aatatcctcc
     4501 ggaattcgtt gtttgcagct tttctgttga aattacgtta acttgctgag aaaaagcgaa
     4561 ctgagaaagc aagataaaca agagtagtag ttttttcatg aaattatatg ttaataatta
     4621 taagttaatg gaaatatatt caaaggttta caaaactaag tataattatt tggataaaat
     4681 aaaaaaagca gatttttttg agatctttcc ctaatgaaat ttaatgaatt gttaatattc
     4741 tgaacaataa cattctaagg ttgcaaatta cttttataag atttagatat tacaggggat
     4801 actgtttatt aaaaagtatc tgacaggaaa taatattggt tctttcgtaa tttattcttc
     4861 atgcttttta catagacaat ttgactgata tttcgtggtt ttacaaaatt ttctttcaat
     4921 tcttttgttt acatttgcac atcggataaa aaacaaataa cgagataaat atgaaaacac
     4981 tggaaccaac aattgtttgg aattattttt atgagataac tcaaattccg cgaccttcaa
     5041 aaaaagagga taaggtcaga gcttacctcg aagactttgg taaaaaaaat aatctgccaa
     5101 ctgtaataga taaagcagga aatgtactga ttaccaaacc tgcaactccg ggttatgaag
     5161 acgggaaaac tctgattctt caggcacaca tggatatggt ctgcgaaaaa aacagcgatg
     5221 tggaacataa ttttgaaaca gatcccatac agattattga agacaatggc tggattaagg
     5281 caaaaggaac tacgctggga gccgataatg gcatcggaat ggcttacatg cttgctgtgc
     5341 ttgcctctga caatctgcag catccggcgg tggaatgtct gtttaccact gatgaagaaa
     5401 cgggtctgac cggtgcattt gcactggatg ataatatgct gaaaggagat attcttatca
     5461 atctggactc ggaagacgat ggtgagattt tcgtaggttg tgctggcggt attgatacta
     5521 cagctactct caaatacaaa ataaaagata ctccgcgtca cttttttgcc ttttcggtaa
     5581 ccgtaaaagg attgctcgga ggtcattcgg gcgacgatat tgacaaacgc cgggccaatg
     5641 ccaataaact actcaatcgg attctgtgga atatgcacaa agaaatggat ttgcatctcg
     5701 tgaaaatcga cggtggaaat cttcggaacg ctattccgcg tgaagcatca gccgtggctt
     5761 gtgttcctta tgcggataaa gaaaaagttc gtgtaatctt taatcaggtg gcagccgagc
     5821 tggaggatga aaactccgcg gtggaaaccg gtatgcggat tgaactccat tcggaagaat
     5881 tacctgcacg tacaatgacc cgtcggagta ccgcccgaat tctgaatgca atatatgcct
     5941 gcccgcatgg tatcaaaagg atgagtgcag atatgcccgg acttgtggaa acatccacta
     6001 atctggcatc agtgaaaatg caggaagaca aaaaactgct gataacgacc agtcagcgca
     6061 gttcggtaga atcggccaag tatgacattg cacatcaggt ggagtctgtg ctgacgctgg
     6121 caggatgtga ggttactcat ggtgatggct atccgggatg gaaaccaaac ctggaatccc
     6181 agatactgaa agtggcagag aaaagctata aaaaactttt cgatgttcaa cccaaggtaa
     6241 aagcaatcca cgcaggtctg gaatgcggtc ttttcctgaa aaaatatccg catctggata
     6301 tgatttccat cggacctacc atgcgcggag tgcattcacc cgacgaaaga ctggaaatcg
     6361 gaaccgttgc ccgtgtctgg agttggctga cagatattct ggcaacaaaa taaaatctga
     6421 acacatattg agggcgttgt gtaaaccgac gccctcattg tctgtatgca acttaaactg
     6481 tcctttggaa agtaaaaatg accatgctac actgagttat gaagaaacaa aacaaaagaa
     6541 agatcatcaa cgatccggtt ttcgggttta tcaatgtgcc aaatgatttt ttgtacgata
     6601 tcattcagca tccttacttt cagcgattga accgcatccg gcaattggga ttatcatcat
     6661 ttgtatatcc gggtgcacag catacccgga tgctgcattc gctgggagca atgcatttga
     6721 tgggtgaagc tattcatcag ttacgcgaag cagggcagga gattaatgct cacgaagctg
     6781 aagctgtccg ggcttgtatt cttcttcacg acatcggaca tggtcctttc tcgcatacgc
     6841 tcgaaaacag tattgtcagg gatatcgcgc acgaacagat ttcgctctgg atgatgcagc
     6901 ggatgaatga tgagtttggc ggaaaactga cactggctct cgatattttc accaataact
     6961 atcataaaca ttttttacat cagctggtat ccagtcagct ggatatggac agactcgatt
     7021 atttaagccg ggatagtttc tactgcgggg taagtgaggg cattatcgga gccgcacgca
     7081 tcattaaaat gctcaatgtg cataatgaca agctggtggt ggaagcaaag ggaatttatt
     7141 caatcgaaaa atttctggtt gcccgccggc tgatgtactg gcaggtctat ctgcataaaa
     7201 catccattgc cgctgagata atgctgataa gtatcctgcg cagagccaag gaactggctt
     7261 tgaagggtga agtattgttt ggaccgcctt ctttgctgta ttttctgtca ggggatatta
     7321 cactaaacca ttttgagagt tcgcctgaaa cacttgatca ttttgcacaa ctcgatgact
     7381 ccgatatcct gagtgcggtt aaagtctgga gtagtcatcc cgataaggtg ctttccacac
     7441 tctgtcacga tttttcgaac cgtaaacttt tcaaagtgga aattctgaca gagaaagaga
     7501 aaggagagat attgcgaaaa cattatctgg aaaaatatca acgtcatttc aatatcagtg
     7561 aacaggaagc cggatatttt ctgggcgtcg aaacggtttc caccgatacg tacagtccca
     7621 aagatgacac catcgatatt ctgctgaaag acggcactgt caaagacatt gctgaagctt
     7681 cggatatgct gaatatacag gttttgacca aaaaagttga aaagcatttt ttctgttatt
     7741 gcaggctgtg acttgtgaaa atgctgattg gcttgttgct cctgaaagta aacaaaacgg
     7801 gcatccaggc tgtcgttctg aaattgaagg gttgaatata tagataattg gaatttcttt
     7861 tgtaattttg caaactaaat tttaacagta tggagttttc tgctcaacaa attgcatctt
     7921 ttctgaacgg cgaaatcgtt ggtaatccaa cagtaacagt aagcaatttt tcgaaaattg
     7981 aagaaggtaa acccggaacg ctaagttttt tatccaatcc caaatacagc cattatatct
     8041 acgactgcaa ttcggacatc gtccttgtaa atcgcagttt cgtgcccgaa agagatgtaa
     8101 aagcgacttt gataaaagta gaaaatgcct acgagtcact tgccgtttta atgggaattg
     8161 tcgaagaata taaaccaaag aaaaacgggg tctcatctct ggcatatatt gctccaacag
     8221 ccaaaatcgg ggcaaatgta tatgtggcgc cttatgtgta tgttggtgat aatgctgaaa
     8281 ttggcaatta ttccacgcta gactctttta cttacgttgg cgacggagcg aaagttggaa
     8341 ataactgcca gctgcatgcc ggtgtgaaaa ttgaaaaaga atgtatcata ggaaatagtt
     8401 gtatattgca tgccggagtt gtaatcggtt ccgatggatt tggttttgca cctactgatg
     8461 atggttcgta tcgtaaaatc ccgcaaaccg gtaatgtgat tatcgaagat aatgtcgaga
     8521 ttggggccaa tagcactatt gaccgtgcca ccctgggcag tacaatcatt cacaaagggg
     8581 tgaaaattga taatctggta caaattgccc acaacgtaga aatcgacgaa aacaccgtca
     8641 ttgctgccca atcgggtgta gccggatcaa ccagactcgg taaacaatgt gtactcgccg
     8701 gacaggttgg aattgccgga catcttgaaa ttgccgacgg cagcgtgttt ggagcgcaga
     8761 caggagttcc cggatcattg aaaactccaa atcagatata tcagggctat cctgcggtac
     8821 cggtcgcaac tttcagacga gcttccgttg tatataagaa tttgcccgaa ttacagaaaa
     8881 ctgtttacga actccagaaa cagctggcag aactgcaatc taaaatcaaa taagtattct
     8941 caaaaatata attatgaatt tgtaaggtaa ctgttctgat tatcaggata caaggacctt
     9001 cattcattaa tttattctat agatatggct actcaaaaaa cattgaaatc atcctttgaa
     9061 ttaaaaggaa aaggactgca taccggtctg gaaatagaaa taaaatttca tccggctgca
     9121 ccatctacag gcatcagaat aaaacggaca gatatggaag caaagcccga aataagtgct
     9181 cttgccgatt atgtgactga taccaatcgc ggaacagtac tgaaacgcgg tgaatttcag
     9241 gtaagtacca tcgaacatgc aatggcagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnna tactttccaa tcagtttgca tccatcgatt cgctcgaaga gttcgaaaaa
     9421 gaaatagccg gagcaagaac atttgtcttt gtgcgggaaa tcgaaccttt gctcaaacaa
     9481 aatctgataa aaggaggcga cctgaaaaat gctattgtta tttatgaccg tcggatggag
     9541 caggaagagt ttgataaact ggctgacatg atgaatcagc ctcacctgaa agtaggcgag
     9601 ttcggcatca tcagcggtga tctgaaattc gataacgagc ctgcccgtca caaattgctc
     9661 gatgtaatag gtgacctcgc actgacaggt aaacatatac agggtaaagt tatagccaat
     9721 tatcctggac ataaagtaaa taccgaactg gcaaaatata ttcgcaaaga aattaaaaag
     9781 caggaagttc aggcaccggt ctatgacgaa accgtccctc cggtcctcga tacagtggct
     9841 atccgcaaaa tgctgcctca tcgttggcca tttcttatgg ttgataaaat aattgaaata
     9901 cgcgaacgat caataatcgg agttaaaaat atcactgtaa acgagacact gtttatggga
     9961 catttccccg acgaaccggt aatgccggga gtgctgctgg tggaagcaat ggctcagaca
    10021 ggcggactgc ttatgttgag tcaactcgac gaaccagaac gatattctac ctatttcatg
    10081 aaaattgata atgtgaaatt ccgtcagaaa gttgttccgg gcgatacggt tattttccat
    10141 gtttcgctga ttgatgatat aagacgcgga attgctacca tgcgagggta tgcttttgtt
    10201 ggtggggtaa ttaccaccga agccgagttt atggcacaaa ttgtaaaaaa taaataataa
    10261 taaaagacaa agtggtgggg tgttaaatca ttttggagaa ttcatttctg attctcagcg
    10321 atccttccac tgaattctgt attttaaaaa atataattat aaaaatgaaa caacctttag
    10381 catacatcca tccagatgca aaaattgcgc ctacggtagt tattgaacct tttgttacga
    10441 ttgataaaaa tgtggttatc ggtgagggaa cacgcatcgg ttcgaatgta actatcctcg
    10501 aaggtgttcg catcggaaaa aactgtacta tttttcccgg agccgttatc ggggcagttc
    10561 cgcaggatct gaaattcaac ggtgaagatt cactggctat tatcggagac aataccacct
    10621 tgcgtgaatt tgtaactgtt aaccgtggta ctgcttccaa aggtaaaacc gtagttggca
    10681 gtaattgtct gctgatggct tattgccacg tagctcacga ctgtgtcatt ggagatcatg
    10741 taattatgag caattcctgt cagcttgcag gtgaagtgga aatagatgat tatgccgtac
    10801 ttggtggtgg aaccatgatt catcagttta gtcgtatcgg tgcacatacc atgatacaag
    10861 gtggctcaaa agtgaccaaa gatgtacctc cgtttatcac agctggccgc gatccccttt
    10921 cgtacgccgg tgttaattct ataggactga ggcgcagagg ttataccaac gaacaaatcc
    10981 gggatattca ggatgtatat cgttttattt tccagtcggg gatgaatatc tcccattcgg
    11041 tagagcgtgt acaagctgaa ctttcggcaa ctaaggaacg ggatgaaata cttttattca
    11101 tcaaaaattc accccgtgga attctaaaag gttatttcga ttagtttcat tttaataatc
    11161 cgaacgtgtc cggatataaa taatagataa ttatgacaac tgataataat gcagaagaaa
    11221 ctccgaaaag gagtttgaat ttcatagaac aaattgtaga agaagacctt tctgcaggaa
    11281 agaacaacgg tagaattcaa acccgtttcc caccggagcc gaatggttat cttcatatcg
    11341 gtcatgccaa ggctatttgt atggattttg gcattgcccg ggattataat gggatttgca
    11401 accttcgatt cgacgacacc aatcctgtaa aggaagacgt ggaatacgtt gattctatta
    11461 tggaagatat aaaatggctg ggttacgaat gggaaaatgt atattatgct tctgattatt
    11521 tccaacagtt gtgggatctg gctgtgaagc tgatcaaaga tggtaaagca tatgtcgacg
    11581 aacagtcggc cgaaaccatt gccaaacaaa aaggtacacc tactgaaccc ggaactgaaa
    11641 gtccctatcg gaatcgtcct gtagaagaaa atctcgcttt gttcgaaaaa atgaataccg
    11701 gagagatacc ggaaggtgct atggtactgc gtgccaggat tgatatggct tcaccgaata
    11761 tgcatttccg cgatccgatt atgtatcgtg tcatcagttc acacccacat caccgtaccg
    11821 gttttacatg gaaagcctat ccaatgtatg actatgctca cggacagtcc gattatttcg
    11881 aaggagtgac acattccatc tgtactctcg aatttgtagt tcaccgtccg ttgtacgatt
    11941 ggtttatcga tcagtttcag gataccgatt accgtccgcg acaaattgaa ttcaaccgac
    12001 tgaatataac ttataccgtg atgagcaagc gtaaaatgct gcaattggtt caggaaggtt
    12061 tggtaagcgg ctgggacgat ccgcgtatgc ctacactttg cggattgcgt cgtcgtgggt
    12121 ttactcctca gagtat
//
LOCUS       DAOPYB010000084        11970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6012_length_11970_cov_9.645069, whole genome shotgun sequence.
ACCESSION   DAOPYB010000084 DAOPYB010000000
VERSION     DAOPYB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6012_length_11970_cov_9.645069"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..741)
                     /locus_tag="PKH58_07695"
     CDS             complement(1..741)
                     /locus_tag="PKH58_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNX88946.1"
                     /translation="MKHLFFNLLFLTGFLSLNAQNYPSGKSILDKIDANMSAKSMIMK
                     SSMEITGSRATRTMEIKTWIVGDDKSFTEYLSPAREKGTKMLKLNKQLWIYSPSTDRT
                     IQISGHMLRQSMMGSDMSYEDMMNDTPLQEQYKAEVTGEENIEGRKCWVVNLTSTVTD
                     INYYSQKFWVDEERYVPLKMQQFAKSGKQLKEITLSDIRKVQGRWYPMVMIYKDTLKE
                     GKGTRFTISGIQFDVAIPENVFNKGNLK"
     gene            complement(893..2161)
                     /locus_tag="PKH58_07700"
     CDS             complement(893..2161)
                     /locus_tag="PKH58_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNX88947.1"
                     /translation="MLKFLYKGLIRDRSRSLLPIIVVTLGVMITVVLQSYMTGVLGDS
                     IETTANFTTGHVKVMTSAYSKNMSQFPNDLAIMDVDVLKKKLQTEFPDVEWSERVQFG
                     GLLDVPDENGETRAQANVAGIGIDMLNSPREINRMNLRSNLTSGRFPDKQGELLISEE
                     LFRRMKLKQGDEVTLIASSMYGEMSMYNFRVCGTLKFGIPFLDRGTIIADFRDVQTAL
                     NMENATGELLGFFKNQFYDNNKAITMAETFNSGQKSGDKFTPVMLPLSEMNGMGYIVA
                     YSENIQGIILLIFIIAMSIVLWNAGLIGGLRRYGEFGLRLAIGESKNEIYRAMIAEAF
                     IIGMIGTIIGVISGLFISWLMQRYGLNIGEMMKDSTILMPTVLRSRITAASYYIGFIP
                     GILSTVIGAMLAGIGIYKRQTANLFKELES"
     gene            complement(2201..3370)
                     /locus_tag="PKH58_07705"
     CDS             complement(2201..3370)
                     /locus_tag="PKH58_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNX88948.1"
                     /translation="MILAKTAIKSLLGNGLKTWLNVFVLSISFVLIIFMQGLLEGWSK
                     QAVTDAVAWEIAGGQYWSSKYDPYDPFSLDSSAAAIPPSLMKEYNAHQIEPVLIASGS
                     IYPDGRMMPVLMKGIRPDQNLLTMPTQSLKGDSTEIPAIIGAHMASQAGLKENDLVTL
                     RWRDANGTFEAVDIRIASVFKTTVPTTDNGMIWLPLENLQNMMSQPGKATMLIKDQQL
                     TTVTVPGWKFKNQTELTKQTIALVRAKSIGMSIFYFIFLFLALIAIFDTQTLSIFRRQ
                     REIGTYVALGMTPRQVQNFFTLEGTMNALLAILLGFTYGLPFFIWFAANGITMPIDAS
                     SFGLPIADKMYPLFTPKLIIGTIIFIFIVTAIVSYLPARKISKMNPTEAIRGKVL"
     gene            complement(3381..4070)
                     /locus_tag="PKH58_07710"
     CDS             complement(3381..4070)
                     /locus_tag="PKH58_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX88949.1"
                     /translation="MKTIVSVKDLVKKYPIGSSGYFTALNGINLEFTESEFAGLVGPS
                     GSGKTTLLNIIGSLDVPSGGEVSVLSHSISTLTQKEAAQLRKEKLGFIFQNYNLLPVY
                     TVFENVEFPLLLLNMSAEERKKRILETLEWVGLSDKINSRPSQLSGGECQRVAIARAM
                     VKRPSLVLADEPTANLDSANSHNVLQTMVKLNRELKTSFIFATHDEKVIGYLHRIIRL
                     EDGKVVSDKTI"
     gene            complement(4122..5270)
                     /locus_tag="PKH58_07715"
     CDS             complement(4122..5270)
                     /locus_tag="PKH58_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88950.1"
                     /translation="MKTLLLFFALIAFTAAMAQAPNDTAAIEHAVTFSGQVTAWEITH
                     FSNPVLWQTGGRFVPALLGNFKTGENSKLDFEASIHINCNLNYTGCDLSDKQGEIQPY
                     RMWLRYSTNRWEIRGGLQKINFGTAKMFRPLMWFDGMDVRDPLKLTNGVYGVLGKYFF
                     KNNANIWIWSLVGNSKPKGWELFGSKKWMPELGGRAEIPVLNGEMALSTHIRAVEIPV
                     VGKQLHENRLGLDGKWDVGPGLWFESSIIRLEKNEYNLPRYQDAWNVGADYTVNIGNG
                     IGLTLEYFRYHAGNSFLVNGNSIKLVGSMFTYPLNMLDNLTAMFFYTRNSSNLWFSYL
                     SWNRTYDKFSIYLMAFWNPDVSLPINTTLQGKNLFSGKGLQLMASYNF"
     gene            5497..5958
                     /locus_tag="PKH58_07720"
     CDS             5497..5958
                     /locus_tag="PKH58_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNX88951.1"
                     /translation="MKKQYTYNELLHKAASYCSISEHCISDVDDKLKAWEVDETDRQK
                     IIQQLTDDDFINEKRFSKAFVRDKFHFNKWGKIKISMALREKGLNHDLIASALDVIDD
                     GEYEEMLATLLKSKQHTIKYEFEYEKQGKLFQFAQSRGFENSVIERVLRNI"
     gene            complement(6165..7004)
                     /locus_tag="PKH58_07725"
     CDS             complement(6165..7004)
                     /locus_tag="PKH58_07725"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNX88952.1"
                     /translation="MPEQQQIDFERIATAIEYMYTNFRTQPSLDEVAAHVSMSPFHFQ
                     RMFQQWAGVSPKKFMQYLNVSHAGNLLRSNQLSLFDAASETGLSGTGRLYDLFVKIEG
                     MTPGEYKNGGRNLNICYCFSPTRFGEMLVASSGKGICYLAFSEDKDRMLTELNELFPN
                     AAYSEQTDELIKKVIRFFGLNWNNPEEIKLHLKGTPFQLKVWETLLKIPEGRLATYGE
                     IAEKINNPKATRAVGSAVGDNPVSFLIPCHRVIRSSGVLGQYHWGPSRKKAMIGWEAV
                     RKD"
     gene            complement(7081..>9309)
                     /locus_tag="PKH58_07730"
     CDS             complement(7081..>9309)
                     /locus_tag="PKH58_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX88953.1"
                     /translation="NATNSDNFNLFRKLTLQNVLEPTGMILLQIIAILVVSRIFSSLF
                     KRIGQPTVIGEIIAGIVLGPSLLGYIFPDTFQFLFSPKYLDTLYIVSQIGLILFMFTV
                     GMDLNMSELKVKLKQTFVISQTSIIIPFLMGMILSYFIYIEFAPASTPYMSFALFIGI
                     SMSITAFPVLARIVQEKGLSKTHLGIVSIGSAAINDVTAWILLVAVIAIANTGDFSSS
                     LYTLIFTVIYTILMIFVIRPFLKRIGNMYQKEELMNKNIFGFFILILIISAYTTQTLG
                     IHALFGAFLAGLIMPPLPMFRKLMMDKIEDLSVTLLLPLFFVYTGLKTEIGLLNTPEL
                     WMLCLVFILVAVIGKFGGGAVTARITGESLKDSLSLGILMNTRGLMELVVLNIGLEMG
                     ILPPVIFTMLVIMALVTTFMATPLLSLIEYIFPAKEKKETLIRQQAQGVFKAMIALGN
                     PENGKNLLRVAKTILDGAKNSLEVNVLHITAGTDTNPIYGEQFSVESFIDVQAASQKL
                     NIPITTEYKVADIIETEIIKKVNAENYDFLLIGAGASMSQDELMKEQRWYDRIPWLDR
                     LRTEQAIFYPGTLIRDKTRYFIENCNCSVGIFVNRDFKGISSILIVLGEENDDFLLRY
                     ARRILRDNPDITVQIIDISKLDARIQTVSEGIQKLTSLFGSRVKLSKITRLHSGYLAK
                     FSFLMISYQTWDALIQAETQTLKDIPSTLIISKRTSRFSKGKRKHVQLESTNADDDYV
                     EV"
     assembly_gap    9310..9409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9434..10495)
                     /locus_tag="PKH58_07735"
     CDS             complement(9434..10495)
                     /locus_tag="PKH58_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNX88954.1"
                     /translation="MFQQIFLNNTLETWLISLAIIAGALLLNKLISLLNRKVILKLTG
                     KTANRFDNILFSTLEAPLMLGIMLVALWVALNRLNIVEKNVKNILSESYQVLAILNIT
                     WFFAKLMVALMDEYLSSSNPKNAHRKVHLDIHMVALSKKTVLFVIWILGGVMALSNIG
                     VNIGALLGTLGIGGVAVALAAQDTVKNIFGGFTILMDGTFRIGDRIKIGEFDGFVENI
                     GIRSTRIRNFDKRIITIPNYKIVEGAVENISEAPSTRVVMSLGLSYSTTTNKMQLASA
                     ILKQTAEEFEHIDKDNISVAFSEFAATSMNLKFTYFISKDGDNVKIPSDINFKILERF
                     NSSGIEFAVQPQSVITFKS"
     gene            complement(10541..>11970)
                     /locus_tag="PKH58_07740"
     CDS             complement(10541..>11970)
                     /locus_tag="PKH58_07740"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNX88955.1"
                     /translation="NFTFGIEICEDLWMPIPPSSHLAIQGAEVLFNLSASNELIAKNI
                     YRQQLVEQQSARCIAGYVYTSAGHGESTTDLVFSGNTLIAENGKVLAKGERFSFEPQL
                     TITEIDIERLRADRMHNNNFFNEKEENAYFEIDCTDNLSKPGDINFKLTRKVDQHPFV
                     PPMSVRDESCSEIFAIQVGGIAKRWQHTHAENLIIGISGGLDSTLALLVCVKTADKLG
                     YDRKRIMGVTMPGFGTTDRTYNNALELMKSLGITMKEISIKEACLLHFRDIGHDPKVH
                     DVTYENVQARERTQILMDLSNKYNGLVVGTGDLSESALGWSTYNGDHMSMYAVNSGVP
                     KTLVRYMVGWAAHQLDDHSEKILTDILDTPVSPELLPFDENGEIAQKTEDIVGPYELH
                     DFFLYYFVRFGFTPEKILFLAKTAFEGKYPDDFIEKWLKVFLKRFFQQQFKRSCIPDG
                     PKVGSINLSPRGDWRMPSDAVAFQF"
BASE COUNT         3290 a         2613 c         2586 g         3381 t
ORIGIN      
        1 ttatttcaaa ttccctttat taaatacatt ttcaggaatg gcaacatcaa actgaatgcc
       61 tgaaatggta aaccgggtgc cttttccttc tttcagcgta tctttgtaaa tcatgaccat
      121 ggggtaccat cgtccctgca cttttcggat atcgctcagg gtaatttctt tcagctgctt
      181 gccgcttttg gcaaactgct gcattttaag gggaacataa cgttcttcgt ccacccagaa
      241 tttctgactg taataattaa tgtcggtgac cgttgaggtc aaattcacta cccagcattt
      301 cctgccttcg atgttttcct cgccggtgac ttccgcctta tattgttcct gcaggggtgt
      361 gtcgttcatc atatcttcgt acgacatgtc gcttcccatc atggactgac gtaacatgtg
      421 ccccgaaatc tggatggtac ggtcggtgga gggagagtat atccagagct gcttgttcag
      481 tttcagcatt tttgtccctt tttcgcgggc tggcgagagg tattcggtga acgatttatc
      541 gtcgccgact atccatgttt ttatttccat ggttcgggtg gcgcggctgc cggttatctc
      601 catcgatgat ttcataatca tggatttggc agacatgttc gcgtcaattt tatccagaat
      661 ggatttaccg gaaggatagt tttgagcgtt caggctgaga aaaccagtta aaaataacaa
      721 gttgaaaaat aggtgtttca taacttcgaa gttaaaaagc cccctaaatc ccccatgggg
      781 gactttcggt gagtgctaaa ttcaagatat ttatttttgt aaaaaataat ttaatgttca
      841 acatagtttg atataacata tctttaagtc cccctagggg atttaggggg ccttaacttt
      901 ccagttcctt gaaaagattg gctgtctgtc gtttgtaaat gcctatgcct gccagcatgg
      961 cacctataac ggttgagagg atgccgggaa taaatccgat ataatacgaa gctgccgtaa
     1021 tgcgtgacct caggacagtg ggcatcagta tggtggagtc tttcatcatc tcaccgatat
     1081 tgaggccata ccgctgcatc aaccaggaaa taaataatcc ggatattacg ccgataatcg
     1141 taccgatcat cccgataata aaagcttcgg caatcatggc gcggtaaatc tcatttttgc
     1201 tttcgccaat ggcaagccgg agaccaaatt cgccatagcg ccgcaatccg ccaatcagtc
     1261 ccgcattcca cagcacaatt gacatggcga tgatgaaaat cagcaggata atgccctgga
     1321 tattttcgga gtaagctaca atgtatccca tgccgttcat ttcactcaga ggaagcatca
     1381 caggagtaaa cttatcaccg ctcttctgtc cggaattaaa tgtttcagcc atcgttatgg
     1441 ctttgttatt atcgtaaaac tgatttttga agaaaccaag caattcaccc gtggcatttt
     1501 ccatgttcag cgctgtctgc acatctctga agtcggctat gatggttccc cgatccagaa
     1561 aaggaatacc gaatttcagc gttccgcata ccctgaagtt gtacatcgac atttcgccgt
     1621 acatggagct tgcaatcaat gttacttcat caccctgttt cagtttcatc cttctaaaaa
     1681 gttcttcgct gatgaggagt tcaccctgtt tatccggaaa tcgtccactg gtcaggttcg
     1741 aacgcaggtt catccggttt atttcacggg gagagttcag catatcaata cctattccgg
     1801 caacattggc ctgagcacgg gtttctccgt tttcgtcggg gacatcaagc agtccgccaa
     1861 actgaacgcg ttcgctccat tcaacatccg gaaactctgt ctgcaatttt ttcttcagca
     1921 catccacatc cattattgcc aggtcattgg gaaactgcga catgttcttg ctataagcgc
     1981 tggtcattac tttcacatga ccggttgtaa agttagccgt cgtttcgatg ctgtcgccca
     2041 gaacgccggt catataggac tgaagcacta ccgttatcat cacccccaga gtgacgacaa
     2101 tgatgggtaa caaactgcgg cttcggtcac ggataagtcc tttatataag aatttcaaca
     2161 taatatctgg ttgattgaat gtctcctgta aattaatttg ttataaaacc tttcccctga
     2221 tagcttctgt cgggttcatt ttcgaaatct ttcgcgcagg cagataactg acaatggctg
     2281 ttacaataaa tataaagatg attgttccga taatcagttt cggagtgaaa aggggataca
     2341 tcttgtctgc aatcggaagt ccgaaactac tggcatcgat tggcattgtg atgccattgg
     2401 ccgcaaacca gataaaaaag ggcagtccgt aggtaaaacc taaaagaatg gcaaggagcg
     2461 cgttcatcgt tccttcaagc gtaaagaaat tctgaacctg ccgcggtgtc attcccagcg
     2521 ccacatacgt tcctatttcg cgctgccgac ggaaaatgga caatgtctgc gtatcgaaaa
     2581 tggcaatgag cgccagaaaa aggaatatga aatagaaaat gctcatgcct attgatttgg
     2641 ccctgaccag cgcgatggtt tgtttggtga gttcggtctg attcttaaat ttccaaccgg
     2701 gaacagtcac cgttgtcagt tgctgatcct ttataagcat ggtggcttta ccgggttgcg
     2761 acatcatatt ctgcagattt tccagcggca gccagatcat tccgttgtcg gttgtgggga
     2821 ctgttgtttt gaaaacggaa gcaatccgga tatcaacagc ttcaaaagtg ccgttggcat
     2881 cgcgccagcg cagggtgacc agatcattct ccttcagtcc cgcctgactg gccatgtgag
     2941 cgccaatgat agccggtatt tccgtactgt cacctttcaa gctttgagtt ggcatggtga
     3001 gcaggttttg atccggacga atacccttca tcagtacagg catcatccgg ccatcgggat
     3061 aaatgctgcc cgaagcaatc aaaacaggtt caatctgatg cgcgttgtat tctttcatca
     3121 atgaaggagg aatggcagcc gcacttgaat cgagcgagaa agggtcgtaa ggatcgtatt
     3181 tactgctcca gtattgtcct ccggctattt cccaggccac tgcgtcggtc actgcctgct
     3241 tgctccagcc ttccagtaat ccctgcatga agataatcag tacaaaggag atggaaagta
     3301 caaaaacatt cagccaggtt ttcagaccat tgcccagcag gctttttatg gcggttttag
     3361 caagtatcat acctgatgca ttaaatagtt ttatcactga caaccttacc atcttccagc
     3421 cggataattc ggtgcaggta tccgatcacc ttttcgtcgt gagtggcaaa aataaagctg
     3481 gtttttagtt cccgattaag tttcaccatg gtttgaagca cgttgtgtga attcgccgag
     3541 tcaaggttgg cggtaggctc atcggccagc accagcgaag ggcgtttcac catggcgcgg
     3601 gcaatggcta cacgttgaca ttcgcctccc gaaagctgcg atggccgtga attgatttta
     3661 tcggataaac caacccattc cagagtctcc agaattcgtt tctttcgttc ttcagcgctc
     3721 atgttcagca atagcaatgg gaattctaca ttctcaaata cggtataaac aggcaggagg
     3781 ttgtaattct gaaaaataaa ccccagtttt tcttttctga gctgcgccgc ttctttctgg
     3841 gtgagcgtgc tgatagaatg gctcagcacc gagacttctc cgccagaagg tacgtcgagt
     3901 gaaccgataa tattgagcag tgtggttttg cccgaaccgc tcggaccaac cagtccggcg
     3961 aattcacttt cggtaaattc caggtttatt ccgttcagcg ctgtaaagta accggacgaa
     4021 ccgattggat atttcttgac taaatcttta acgctaacaa tagttttcat atgcagtatc
     4081 gctgtttagt tgtatcattc aataagtata attttataat atcagaaatt gtagcttgcc
     4141 atcaactgca acccttttcc cgaaaacaga ttttttccct gtaaagttgt atttatcggc
     4201 aaactgacat ccggattcca gaatgccatc aggtaaatgc tgaatttatc ataggtacgg
     4261 ttccagctca ggtaactgaa ccacagatta ctgctgttgc gggtgtaaaa gaacatggct
     4321 gtcaggttgt cgagcatatt caggggataa gtgaacatgc ttcccaccag tttaatgctg
     4381 tttccgttta caagaaaact gttacctgca tgatacctga agtattccag cgtcagacca
     4441 attccgtttc caatatttac cgtataatct gctcccacat tccaggcatc ctgatagcgt
     4501 ggcaggttat attcgttctt ttcaagtcgt atgatgctcg attcgaacca cagtccggga
     4561 ccgacatccc attttccatc cagtcccaga cggttttcgt gtaattgttt cccgacaacg
     4621 gggatttcta cagcgcgaat gtgagtgctg agcgccattt cgccattcaa taccggtatt
     4681 tcagcccttc ctccaagttc cggcatccac ttttttgaac caaacagttc ccagcctttc
     4741 ggtttgctgt tgccaaccaa agaccagatc cagatattgg cattattctt aaaaaaatac
     4801 tttcccaaca cgccgtaaac accgtttgtg agttttaacg ggtcgcgtac atccatcccg
     4861 tcgaaccaca tcagcggacg aaacattttt gccgtaccaa agttgatttt ttgcaatccg
     4921 ccccgtatct cccagcggtt tgtcgaatat cgcagccaca tccggtaagg ctgaatttca
     4981 ccttgcttgt cggatagatc gcatccggta tagttcaggt tacagtttat gtgaattgaa
     5041 gcctcaaaat ccagttttga attctcaccg gttttgaagt taccaagcag ggcaggcacg
     5101 aagcgtccac cggtttgcca gagcacggga ttgctgaagt gtgtgatctc ccaggctgtg
     5161 acctgaccgc taaaagtgac agcgtgttca atggcggcgg tgtcgttagg tgcctgagcc
     5221 attgcggcag taaaagcaat cagagcaaaa aacagtaaaa gtgttttcaa acaggccttc
     5281 atttcattaa cctgaaaata aactgaataa aacgttaatt cggttgtaaa gctacaaaag
     5341 attgtttgtt ttccaactat tacagagctt ttctttacga tgaaatattg ttgttcaaaa
     5401 aaatatattt cattttttta tacttcaaaa aaataactac atttgtctgt cacaaccaat
     5461 ggatataaat ttcaattatt ttattgcctg taagccatga aaaagcaata tacatacaac
     5521 gaactgctgc ataaggcggc atcgtactgt tccatttccg aacattgtat ttctgatgtt
     5581 gacgataagc tgaaagcctg ggaagtggat gaaactgacc gccaaaaaat cattcaacag
     5641 ctaaccgatg atgactttat caacgaaaaa aggttttcga aagcatttgt acgggataaa
     5701 tttcacttta ataaatgggg gaaaatcaaa atttctatgg ctttgaggga aaagggcctc
     5761 aatcatgatc tgatagcgtc ggcactggat gtgattgacg atggtgaata cgaagaaatg
     5821 ctggcaacct tattaaaatc aaaacaacat acgataaaat acgagtttga atatgaaaaa
     5881 cagggtaaac tgttccagtt tgcgcaaagt cgtggtttcg aaaacagtgt gattgaacgg
     5941 gtactcagga atatttagac acaagacaca agacacaaga tacaagacac aggatgtaag
     6001 acacaagata caagatacaa gatacaagat acaatacagc ctcaaacctc ccgtaacaag
     6061 tttccagata gcttcccgat agatatggac aggctctgaa ggggcttaaa gagaataatc
     6121 aaataactgt caattcttga acccccttga gggggcaggg gggcctaatc tttcctaacg
     6181 gcttcccatc caatcatggc ttttttgcgg gatggtcccc agtggtattg tcccaacaca
     6241 cctgaagaac gaataacccg atggcacgga atgagaaaag atacaggatt atcgccgacg
     6301 gcagatccta cagcgcgggt tgctttggga ttattgattt tttcggctat ctcgccatag
     6361 gtggcaagcc ggccttcggg tatcttcagt aacgtttccc agactttgag ctgaaagggg
     6421 gttcctttca ggtgcagttt tatttcttcg ggattattcc agttaagtcc gaaaaaccgg
     6481 atcacttttt taatgagttc atcggtttgt tcagaatagg cggcattggg aaataattcg
     6541 ttaagttcgg tcagcatcct gtccttatct tccgagaagg caagatagca aattcccttt
     6601 ccggaggagg caacaagcat ttcgccgaag cgggtgggag agaagcagta acagatattc
     6661 aggtttctac ctccgttttt atattcgcct ggtgtcattc cttcgatttt tacaaacaaa
     6721 tcgtacaatc ttcccgtgcc ggaaagtccg gtttcagacg ctgcgtcaaa gaggctcaac
     6781 tggttgctcc ttaataaatt tccggcatga ctgacattca gatattgcat gaattttttg
     6841 ggacttacac cggcccattg ctgaaacatt cgctgaaaat gaaacgggct catgctgaca
     6901 tgcgccgcca cttcgtccag actgggttgt gtccggaaat ttgtgtacat atactcgata
     6961 gctgtggcaa tgcgttcgaa gtcaatttgt tgctgctcgg gcatggagga tggatgtttt
     7021 cagtaaaatt acacaatcgg tacaaaatca acaacccgat tcttgcggat tttcagctgt
     7081 tcagacttcg acatagtcat catccgcatt ggttgattcc agttgaacgt gttttcgttt
     7141 gcctttactg aatctgcttg ttcgtttgct gataataagt gtagaaggga tgtctttaag
     7201 tgtctgcgtt tcagcctgaa taagtgcatc ccatgtttga tagcttatca tcagaaaact
     7261 gaatttggca agatatcctg agtgcagacg agtgatttta ctcagtttaa cccgtgaacc
     7321 aaacaagctt gtaagcttct gaataccttc agatacagtt tggatacgtg catcgagttt
     7381 gctgatatca ataatctgaa cggtaatatc cggattgtcg cgcaggatac ggcgcgcgta
     7441 cctgagcagg aagtcgtcat tttcttcacc caaaacaatc agaatagaac ttatcccctt
     7501 gaaatcgcgg ttgacaaaaa tgccgacact gcaattgcag ttttcgataa aatacctcgt
     7561 tttgtccctt atgagtgtac ccggataaaa gattgcctgt tcggtcctta atctgtccag
     7621 ccagggtatg cggtcgtacc agcgttgttc cttcatcagt tcgtcttgac tcattgaggc
     7681 accggcaccg attagcagaa agtcgtaatt ttcggcatta acttttttga taatttcagt
     7741 ttcgataata tcggcgacct tatattcggt agttattggg atgttaagtt tttgtgaagc
     7801 agcctgaaca tcgataaaac tttcaactga aaactgttcg ccgtaaatcg ggtttgtgtc
     7861 ggttccggca gtaatgtgaa gcacattcac ttcaagcgaa ttttttgctc cgtccagaat
     7921 ggtctttgcc acccggagta aatttttacc gttttcggga ttacccaggg ctatcattgc
     7981 tttgaaaaca ccctgagcct gctgacgaat aagtgtttct ttcttttctt tggcagggaa
     8041 aatatattca atcagcgaaa gcagcggagt agccataaag gtagttacca atgccatgat
     8101 aaccaacatg gtaaaaataa ccggcggaag aatacccatt tcaagaccga tattcagaac
     8161 gaccagttcc atcagaccgc gcgtattcat caggataccg agagacaggc tgtctttgag
     8221 tgattcgccg gtaattcggg cagtaacggc accgcctccg aattttccaa taacagctac
     8281 cagtatgaat accaggcata acatccacaa ttcaggagta ttcaacagtc ctatttccgt
     8341 tttcagacca gtatatacga agaataaagg caggagcaag gtaacagaga gatcttcaat
     8401 tttatccatc atcagtttac ggaacatggg gagtggcggc atgataagac ctgcaagaaa
     8461 agctccgaac aaagcgtgaa ttcccagagt ttgggttgtg taggccgaga tgataagaat
     8521 cagaataaaa aatccgaaga tatttttatt catcagttcc tctttctgat acatgtttcc
     8581 aatgcgtttg aggaacggac gaataacgaa tatcatcaaa atagtgtaaa ttaccgtgaa
     8641 aattaatgta tataaagaac tggaaaaatc accggtatta gcaattgcta ttaccgcgac
     8701 caataatatc caagctgtga catcgttaat tgccgcgctg ccaatgctga caattcccag
     8761 atgagtttta gaaagtcctt tttcctgaac aatacgtgcc aaaaccggga aagcggtaat
     8821 actcatggat atgcctataa acagagcaaa tgacatgtat ggggtacttg ccggagcaaa
     8881 ttcgatgtaa atgaaataag ataaaatcat tcccatgaga aacggaatga ttatacttgt
     8941 ctggctgata acgaaagtct gcttgagttt cactttcagc tcactcatgt ttaaatccat
     9001 accaacggta aacataaata aaatcagtcc gatctggctg acaatataga gtgtatcgag
     9061 gtattttggt gaaaacagga actgaaaagt gtcggggaaa atataaccga gtagtgacgg
     9121 tcccaaaaca ataccggcaa taatttcacc gataacagta ggctgcccaa ttcgtttgaa
     9181 gagtgaacta aagatacggg ataccaccag aatggctatt atttggagca gtatcattcc
     9241 ggtcggttcc agaacgtttt gtaaagtcag tttccggaat aaattaaaat tgtcagaatt
     9301 agttgcattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aaaagttaca
     9421 taaagtataa aaattatgat ttaaacgtga tcacagattg tggctgcacc gcgaattcta
     9481 taccggagga attaaatctt tccagaattt tgaagttgat atccgaggga attttcacgt
     9541 tatcgccatc tttggagata aaatacgtaa atttcaggtt catggaagta gctgcgaatt
     9601 cgctgaaagc aacggaaata ttgtctttat cgatatgctc aaattcttct gcagtctgtt
     9661 tgagaatagc cgaagccagt tgcattttat tggtggtggt ggagtaggaa aggcccagac
     9721 tcataaccac tcttgttgat ggcgcttcag aaatgttttc gactgcacct tccactattt
     9781 tgtaattcgg gatggtaatt atccgtttgt caaaatttct gatacgggta cttctgattc
     9841 ctatattctc tacaaatcca tcaaattcgc cgatttttat ccggtcgcca attcggaaag
     9901 ttccgtccat cagtatggtg aaacctccga aaatgttttt caccgtatcc tgagcagcca
     9961 gtgccacagc cacaccaccg atacccagtg tgccgagcaa agccccgata tttaccccga
    10021 tattgctcaa agccatcaca ccgccgagta tccagatgac aaacaaaacg gttttttttg
    10081 acaatgcaac catgtgaatg tccaaatgca cttttcggtg tgcattcttt ggattggacg
    10141 atgaaaggta ttcatccatg agggcaacca tcagttttgc aaaaaaccag gtgatattca
    10201 gtatagccag tacctggtaa gattcggata agatgttctt tacgtttttt tcaacgatat
    10261 tcagcctgtt cagagccacc caaagggcaa caagcataat gcccagcatc aggggagctt
    10321 ccagcgtgct gaagaggatg ttgtcaaaac gattagccgt tttacctgta agtttaagta
    10381 taactttccg gttgagcaat gagatgagtt tgttcagcag caatgctccg gcaatgattg
    10441 ccagtgaaat caaccaggtt tccagggtgt tgttcaagaa gatttgttga aacataagac
    10501 aaaaaataat acagaataaa cgtgatataa atgtttttta tcagaattga aatgcaacgg
    10561 cgtcgcttgg catgcgccag tcgccccggg gtgagaggtt gatgcttccc acttttggtc
    10621 cgtcggggat gcacgaacgc ttgaactgtt gctggaaaaa tcgtttcaga aaaactttca
    10681 gccacttttc aataaaatca tccggatatt ttccttcaaa agctgtttta gcgaggaata
    10741 aaattttttc aggggtaaat ccaaagcgaa cgaaatagta caggaagaaa tcatgcagtt
    10801 cataaggtcc cacaatgtct tcggtctttt gcgcgatttc accattttca tcaaacggga
    10861 gcaactccgg acttacaggg gtatccagta tatccgtgag tattttctcg gagtgatcat
    10921 ccagctgatg tgcagcccag ccaaccatat agcgtacaag agttttggga acaccgctat
    10981 tcacggcata catggacatg tgatcgccgt tgtatgttga ccatccgagt gccgattcag
    11041 acaaatcgcc tgttccaacc accagtccgt tatacttgtt ggacagatcc atcagaattt
    11101 gcgtacgttc ccgtgcctgc acattttcat aagtaacatc gtgaactttc gggtcgtgtc
    11161 cgatatccct gaaatgcaac agacatgcct ctttgattga aatttccttc atggtaattc
    11221 ccagggattt catcaactca agggcattgt tgtaagtacg gtcagtagtg ccaaatcccg
    11281 gcatggtaac tcccatgatt cgtttgcggt cataaccgag tttatccgcg gttttcacac
    11341 acaccagcaa tgccagtgta gaatccaatc ctccagaaat accgattatc agattctcgg
    11401 cgtgggtatg ttgccagcgt ttggcgattc ctcccacctg aatggcgaaa atttcgctgc
    11461 aactttcatc acgcacactc atgggcggaa cgaaaggatg ctggtcaact ttacgggtaa
    11521 gtttgaagtt tatatctcca ggtttcgata aattatcagt acagtcaatc tcgaaataag
    11581 cattttcttc tttttcattg aagaaattat tgttgtgcat tcggtcagcg cgaagccgtt
    11641 caatgtcaat ttcggtgatc gtcagttgcg gttcgaagct gaaccgttca ccttttgcca
    11701 gaacttttcc attttcagca attaatgtgt ttccagagaa aaccagatcc gttgtggatt
    11761 cgccatgtcc tgctgaggtg taaacataac cggcaataca acgggctgac tgctgctcaa
    11821 caagttgctg gcggtaaata tttttggcta tcagttcgtt actggcagag agattaaaaa
    11881 gaacctcagc tccctgaata gccaggtgtg aactgggcgg tatcggcatc cagaggtctt
    11941 cgcatatttc tatcccgaag gtgaaattat
//
LOCUS       DAOPYB010000085        11968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6015_length_11968_cov_9.339209, whole genome shotgun sequence.
ACCESSION   DAOPYB010000085 DAOPYB010000000
VERSION     DAOPYB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6015_length_11968_cov_9.339209"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1081
                     /locus_tag="PKH58_07745"
     CDS             71..1081
                     /locus_tag="PKH58_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNX88956.1"
                     /translation="MFKDKILLITGGTGSFGNAVLKRFLDTDIKEIRIFSRDEKKQDD
                     MRHAIQNPKVKFYIGDVRDKRSVDGAMRDVDYIFHAAALKQVPSCEFFPIQAVRTNVL
                     GTENVLDSAIQHGVKNVVVLSTDKAAYPINAMGISKAMMEKVAIAKARQLGENAVTTI
                     CCTRYGNVMASRGSVIPLWIDQIQAGKCITITDPNMTRFMMTLDDAVDLVLYAFEHGK
                     NGDLFVQKAPAATLTVLAEALKQLYRADTEVKVIGTRHGEKLYETLVTREEMAKSEDM
                     GNYFRIPADKRDLNYDKYFVEGQEEIAQIDDYHSHNTTRLDVEGMKELLLKLNMKQNT
                     DL"
     gene            1245..2360
                     /locus_tag="PKH58_07750"
     CDS             1245..2360
                     /locus_tag="PKH58_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX88957.1"
                     /translation="MIRVGITGQAGFVGTHLWNHLGLQPDVFERIPFEDAYFTREDVL
                     RNFVRSCDVIIHLAAMNRHPDPQVIYDTNLGLVQQLVHAMESEQVTPHVLFSSSTQEE
                     LDNLYGKSKRDGRRLLEEWARRNQAAFTGLVLPNVYGEFSRPNYNTFIATFAHKLIEG
                     ENPAIISDSTVKLIHVSSLCRFMIDHFHSTGVKRIDVPHDMERKVSEVLALFEEFKAL
                     YIDKGIIPALKDLNDVHLFNTFRSFIDHGKHFPVELVKHTDPRGMFVETIKLGVGGQV
                     SFSTTVPGITRGNHYHTRKIERFTVIKGKARIQLRKIGTDEVMDFFLDGNNPSYVDMP
                     VWYTHNITNIGEEELYTQFWINEWYNPEDGDTYFEEV"
     gene            2463..2855
                     /locus_tag="PKH58_07755"
     CDS             2463..2855
                     /locus_tag="PKH58_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNX88958.1"
                     /translation="MDEVYLHKELTAEIINRFYKVYNTLGYGFLEKVYEKALKYELEK
                     AGFNVERQKPINVYYETELVGEYFADLLVENKVIIELKAAETICEEHENQLINYLKAT
                     EIEVGLLLNFGKKPEVKRKIFTNKLAHR"
     assembly_gap    2936..3035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3059..4210
                     /gene="wecB"
                     /locus_tag="PKH58_07760"
     CDS             3059..4210
                     /gene="wecB"
                     /locus_tag="PKH58_07760"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNX88959.1"
                     /translation="MKKLKVLTVVGTRPEIIRLACVLQKLDASEAIEHILVHTGQNYD
                     YELNEVFFEDLGLRKPDFFLNAAGKNATETAGQILVNIDPVLEEVKPDAFLVLGDTNS
                     CLCAIAAKKRHIPIFHMEAGNRCFDQRVPEESNRKIVDHTSDINLTYSDIAREYLLSE
                     GLRPDRIIKTGSPMYEVLTQYLPKIEKSTILEQLNLESGKYFVVSAHREENINSEKNF
                     SNLVNILNEVAEKYGFPVIVSTHPRTRKMVEQKGIVFHPSVQLMKPMGLSDYNKLQMN
                     AFAVLSDSGTISEESSILNFRALNIREAHERPEAMEEASVMMVGLNPERVMQGLVQLQ
                     YQATGSRSAQSPITGFRPVADYSMPNVSDKVVRIIISYTDYIKRVVWGE"
     gene            complement(4631..4804)
                     /locus_tag="PKH58_07765"
     CDS             complement(4631..4804)
                     /locus_tag="PKH58_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HNX88960.1"
                     /translation="MKELVAKIQEQIDAFSENAGMQVEKNNKAAGARARKASLELEKL
                     LKEFRKVSITASK"
     gene            complement(5140..5745)
                     /locus_tag="PKH58_07770"
     CDS             complement(5140..5745)
                     /locus_tag="PKH58_07770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX88961.1"
                     /translation="MIDHFAHKANDWDQPEKIRMSEKFVEELMKLVDIEPQWKAFEIG
                     AGTGLVGLQLLPMLKSCVFEDTSEAMLDVLRTKVTGLSNVEILHGEVSEYTRPDIDLI
                     ISNMAFHHIPDIAATLHHLHTITTSGAVVVVSDLRAEDGSFHQFEPIPHRGFDAENLT
                     AIFREAGFQVEVLKTYNILHKKQPSGTIGDYEQFILKACRL"
     gene            6259..8319
                     /locus_tag="PKH58_07775"
     CDS             6259..8319
                     /locus_tag="PKH58_07775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX88962.1"
                     /translation="MAIVLSAVVSACDTKKDEGPIIEKQVPVIENGLMTPEVLWAFGR
                     VGNVEISPDETKILYSVSYYSVEQNKSNSELFVMNVDGSEKKQITKTASKEIAAKWMN
                     DSKRIAFLSNESGSMQLWTMNDDGTGRKQLSEREGDISDFSFSPDESKVLLVADVKYG
                     KRTVDIHPDLPQASGVIVNDLMYKHWDEWVQTVPHPFVASVEGGKLTNETDILKDEPY
                     ESPMKPFGGIEQLAWSPDGKTIAYTCRKKTGKEYALSTNSDIYFYDLETGKSVNKTEG
                     MMGYDQNPVFSPDGKWLAWESMEHEGYESDKSRLFVMNVKTGEKKDLSADFDQNSNGL
                     KWSADGQSIYFVSCFHAVTQIYKADLNDGKIMPVTKGVHDYLSVIPLKNQLIGVKQSM
                     SKPDEIYAINAATGEEKELSFENKDILDKLEMGKVEERWIATTDNKQMLTWVIYPPHF
                     DSAKKYPTLLFCEGGPQSTVSQFWSYRWNFQIMAANGYIIVAPNRRGLPGFGYEWLKQ
                     ISGDYGGQNMNDYLSAIDAVAKEPFVDKDKLGCVGASYGGFSVYWLAGHHNKRFKAFI
                     AHDGMFNLPQQYLETEEKWFVNWDLGGAYWDKNNAVAQRSYANSPHLFVDKWDTPILI
                     IHGEKDYRILASQGMAAFDAAVLRGVPAEMLIYPDENHWVLKPQNGILWQRTYFSWLD
                     KWLK"
     gene            8462..8818
                     /locus_tag="PKH58_07780"
     CDS             8462..8818
                     /locus_tag="PKH58_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX88963.1"
                     /translation="MKTHHDLTVWKKSIDFVTTVYKITEEYPKTEIYGLTNQVRRAVV
                     SVPSNIAEGAARTSKKEFSHFLSISLGSISEVETQLIVSRNLNYITNEQLENLLSELI
                     EIRKMIIGLKKTMSDY"
     gene            8931..9482
                     /locus_tag="PKH58_07785"
     CDS             8931..9482
                     /locus_tag="PKH58_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX88964.1"
                     /translation="MDEQRLITGCKRGEQWARREVYEKYAPAMLSLCVRYVADYEAAR
                     DVLQDGFVRVFTKTDQYEGRGNFGGWIRQVFVNTSLEYLRRKNSLQFETRADEINEAN
                     ELQNPESGTLTAEDLLECVAELPDKLRTVFNLYAIEGFSHGEIAAMTGIQESTSRSHF
                     LRARLLLQQKVTELLNNEHAGKK"
     gene            9460..10824
                     /locus_tag="PKH58_07790"
     CDS             9460..10824
                     /locus_tag="PKH58_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX88965.1"
                     /translation="MNMQEKNKHTNDHDAFSQWAKQQLENHQLPVDDEIWKGIEKKLA
                     PAPKRSVPFWYWLAGGVAAVVALMLLLRPFYHSSGRDINTQTIVSQKGANKQVVTSEQ
                     NPVDISVRPQVVEKDHPATGVKSNNSARHSNKNIFIARNSETVKTKQTEEFGGMTAEN
                     FVSDMQTSGNEKEKKERTVSEEHPLTTKAGDEGKNKNDNSKANVSKQQITSLPDLSDY
                     PEEPAVETVKNKKNEHWLLAASLGAGSGISSNGSGLFFETGGLMSDAALVNSTSVKTE
                     YTADIKDADFFNEVQHLPPVSFGLMAELPLNRSWSLESGFVYTYLESRLKRTTNPTAT
                     GHLKLHYLGLPLYLKAKLWHDTSWSIYLSAGPMMEKGLKSIYSQDIDYTWSITHTDVH
                     SRIEGLQWSLNTGLGVNYKLQKDLSVFFEPKLTYYLDNNQPMSARTEQPLVIGLNGGL
                     RIEL"
     gene            11192..11842
                     /locus_tag="PKH58_07795"
     CDS             11192..11842
                     /locus_tag="PKH58_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HNX88966.1"
                     /translation="MRKLNLFAGIIILSVFIGSCTYEEADREKIVEMTIYPETGYGAG
                     VLSDVVTEPIQFSDNDENRKQLLTDIITEGFDFNYERGYEYTLKVKKVWMQNPPQDVS
                     SVKYIFIELLSNKKVITTDSEKCIELFVSSQTVKFTPKYPNEYENVGGIETLKIYNAI
                     HVQEKGTDNWMALTKIEGFDFEKGYEYVINVKKITQSDPYSVRYVLLNIVSKTEKK"
BASE COUNT         3847 a         2187 c         2614 g         3220 t
ORIGIN      
        1 actatttact attgactgtc tcttacttga tgaacttgac taacttgcta acttgataaa
       61 ctaacacaat atgttcaaag acaaaatttt attaatcacc ggtggtaccg gatcgtttgg
      121 aaatgcagta ctgaaacgct ttctcgatac ggatataaag gaaatccgca ttttcagccg
      181 cgatgagaag aaacaggatg atatgcgtca tgcaatacaa aatccgaagg ttaaattcta
      241 tatcggcgat gtgcgtgaca aacgcagtgt tgacggcgcc atgcgggatg tggactatat
      301 ttttcatgcc gcggctttga aacaggttcc gtcatgtgag tttttcccga tacaggctgt
      361 acgtaccaat gtattaggga ccgaaaatgt gcttgattcg gctattcagc acggtgtaaa
      421 gaatgttgtg gtactctcca ccgataaagc tgcctatcct atcaacgcga tggggataag
      481 caaagccatg atggaaaaag tggcgatagc caaagcccga cagctgggtg aaaatgcagt
      541 aactaccatt tgctgtaccc gttatggtaa cgtgatggcc agccgcggat cggtaatacc
      601 gttgtggatc gaccagatac aagccgggaa atgtattacc atcaccgacc cgaatatgac
      661 acgcttcatg atgacactcg acgacgcggt ggatttggtg ttgtatgctt tcgaacatgg
      721 aaaaaacggt gatttgtttg tacagaaagc tcccgctgct acgctgacag tgttagcaga
      781 agcattaaaa caactctacc gggctgatac cgaagtcaaa gtaatcggga cacgccacgg
      841 tgaaaaactt tatgaaacac tggtaacccg cgaggaaatg gcaaagagtg aggatatggg
      901 caattacttc cgcattccgg ctgataaacg tgacctgaat tatgataaat acttcgtgga
      961 agggcaggaa gaaatagctc aaattgatga ttaccattcg cacaacacca cccgcctgga
     1021 tgtggaaggt atgaaggaat tgttgctgaa actgaatatg aaacagaaca cagatttata
     1081 attaattgaa cacagataac actggtttga ctgatttaag ctgataagaa ttctattcta
     1141 attaaaatca gtgctcatct gcttaatcag cgtcatctgt tttttaaaat tagaaatcag
     1201 tgattaatag tataatttgt ttttatatac gtatttcgta gcttatgata agagtaggta
     1261 taaccggaca agccggattt gttggcacac acttatggaa tcacctgggt ctccaaccgg
     1321 atgttttcga aagaattccg tttgaggacg cgtattttac aagagaagac gttctgagga
     1381 attttgtgag aagttgtgac gtgatcattc atctggccgc catgaacagg catccggacc
     1441 ctcaggtcat ctacgataca aatcttggac tggtacagca gctggttcat gccatggaat
     1501 ccgaacaggt aacaccgcat gtcctctttt cgtcatccac gcaggaagag ctggacaatc
     1561 tgtacggaaa atccaaacgc gacggacgcc ggttgctcga agaatgggca cgacgcaatc
     1621 aggctgcatt taccggattg gtacttccca atgtgtatgg cgaattttcc agaccgaatt
     1681 acaacacatt tattgccact tttgcacata aactgattga aggtgaaaat cctgctataa
     1741 tcagcgacag tacagtaaaa ctcattcatg tgagctcgct ttgcaggttt atgattgatc
     1801 acttccatag taccggggta aagcgtatcg acgtgcctca tgatatggag cggaaagtga
     1861 gtgaagtcct ggctttgttc gaagaattca aggcactata catcgataaa ggcataatac
     1921 cggcattgaa agatttaaat gatgtacatt tattcaacac gtttcgttct tttattgatc
     1981 acgggaaaca ttttccggtg gaactggtta agcacaccga tccccggggc atgttcgtcg
     2041 aaaccatcaa gctcggcgtg ggtggacagg tgtcattttc gactactgtt cccggtataa
     2101 caagaggaaa tcattaccac acccggaaga ttgagcgttt caccgttatc aaagggaaag
     2161 ccagaattca attgagaaaa atcgggactg atgaagtgat ggattttttt ctggacggca
     2221 acaacccatc gtatgtggat atgccggtat ggtacaccca caacattacc aatatcggag
     2281 aagaggaatt gtacacacaa ttctggataa acgaatggta caatcccgaa gacggagata
     2341 cgtattttga agaggtgtga gttaatggaa cacagaatta attaattgaa cacagataac
     2401 acagattgaa tggatgaaca cagatgtttt agttgctgat tttacccaaa aaattatata
     2461 gaatggatga agtgtattta cataaggaat taactgcaga aataataaac aggttttaca
     2521 aggtttataa tacgctcggg tatggttttc tggaaaaggt gtatgagaaa gctctgaaat
     2581 atgaattaga gaaagcaggt tttaatgtag aacgtcaaaa acctatcaat gtttattatg
     2641 aaacagagtt ggttggagaa tactttgctg atttattggt cgagaataaa gtgataattg
     2701 agttaaaagc tgctgaaaca atttgtgaag aacatgaaaa tcaactaatc aactatttga
     2761 aagcaacaga aattgaagtt ggattattgc tgaattttgg caaaaaacct gaagttaaac
     2821 gaaaaatatt tactaataaa ttagcacaca gatgacacaa atcgaacaaa taatcactga
     2881 tattaaaata atcttatctg tgtaaaccag ttaaatcagt gtcatctgtg ttccannnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaaa tctgtgttcc ataaaaatat
     3061 gaaaaaacta aaagtcctta ccgttgtggg cacacgcccc gaaataatca ggcttgcctg
     3121 tgtattgcag aaactcgatg cttccgaagc aatagaacat atcctggtgc ataccggaca
     3181 gaattacgat tacgaactca atgaagtctt ttttgaagac ctcggtctgc gtaaaccgga
     3241 cttctttctt aatgctgccg gaaaaaatgc caccgaaaca gccggtcaga ttctggtcaa
     3301 tatcgatccg gtactggagg aagtcaaacc cgatgctttc ctggtattag gcgatacaaa
     3361 ctcctgcctg tgcgccatcg cagctaaaaa acgtcacata cctatcttcc acatggaagc
     3421 tggaaacaga tgttttgacc aacgtgtacc cgaggaaagc aaccggaaaa tagtagatca
     3481 tacttcagat attaacctta catacagcga tattgcccgt gaatacttac tgtcagaagg
     3541 tctgagaccg gatagaataa taaaaaccgg tagcccgatg tatgaagtct taactcagta
     3601 tctgccaaaa attgagaaat caactattct cgaacaactg aatcttgaat ccgggaaata
     3661 ctttgtcgtt tcggctcatc gcgaagagaa tatcaactcc gaaaagaatt tcagtaacct
     3721 ggtgaatata ctcaacgaag tggctgaaaa atacggattt cccgtgattg tttcgaccca
     3781 tccgcgtacc cgcaaaatgg tggaacagaa aggaattgta tttcacccca gtgtgcagtt
     3841 gatgaaaccg atgggactaa gcgactacaa caaactgcag atgaatgctt ttgccgtact
     3901 gtccgatagc ggaaccattt ccgaagaaag ttcaatcctg aatttccgtg cgctgaatat
     3961 ccgggaagcc cacgagcgtc ccgaagccat ggaagaagcc tcggtaatga tggtgggtct
     4021 caatcccgaa cgggtaatgc agggactggt acagttgcag tatcaggcaa caggctcgcg
     4081 ttctgcccag tcaccaatca ccggttttcg tccagtagcc gactacagta tgcccaatgt
     4141 gagtgataaa gtggtgcgga taatcatttc ctataccgac tatattaagc gtgttgtttg
     4201 gggagagtga agaagagggt gtaatgtgta atgtgtagag tgtagagaca ggagcaaaga
     4261 ctttgagact gggagagtat gagaccggga gagaaatcag taatgagtaa tgagtaatga
     4321 gtaatgagta atgagtaatg agtaatgagt aatcagtaat cagtgaacag ttaaacagta
     4381 taagttataa gatagtttat aggcaacaat taaacgattc aacagttaaa cgattcaata
     4441 ttccacactt gatgcctgtc ccgtacttgt ttcgggaaac tttacaaact tgataaactt
     4501 tttactataa actaactctg catattaaaa caaaaaaacg gctggaaatc tgattcagat
     4561 ttttagccgt tatttttttc ggaatacgga aatttagctt ccggtattcg ggaaaactct
     4621 cttcaagaac ttattttgaa gcagtgatag aaactttgcg gaattctttc agcaattttt
     4681 ccaattccaa agaagctttg cgtgcacgtg caccagcagc tttgttgttt ttttctactt
     4741 gcatacctgc attttcagag aaagcgtcaa tttgttcttg aattttagct accaattctt
     4801 tcataaggct ttttatttaa gtttattaaa ataaatctat cacaaaaata caatctttta
     4861 tctttattcc aacacttttt acaaaaatcc cgttaatttt tttgaaaaaa atgcatgttt
     4921 tctcagtttt agctgatttt tcacattttt tagtccggaa agtgaaatat acagccttca
     4981 tttatgatta gtttcagaaa atgtatttgc attttttatg gaattgttca tctgtctgta
     5041 ttttcgaagt gagatgacta atcttaacag tccgcaaaat ggatgatatg agataattta
     5101 ttcgaaccgg ctgtttgtcc tccttatctt agtcagaact tatagtctgc atgctttcag
     5161 aataaattgt tcataatctc caatagttcc ggatggctgt tttttgtgaa ggatattgta
     5221 tgttttcaac acttcaacct gaaagccagc ttccctgaat atggcagtaa gattttcagc
     5281 atcgaatccc cggtgcggta taggttcgaa ttgatggaat gaaccatcct ctgcgcgtaa
     5341 atcgctcacc acaaccacgg ctccggaggt agtgatggta tgcaggtgat gcagggtggc
     5401 ggcgatgtcg ggaatatgat gaaacgccat attggagatt atcagatcga tatcaggccg
     5461 ggtatattcc gatacttctc cgtgaaggat ttccacattc gaaagtcctg ttacctttgt
     5521 tctcagcaca tccagcattg cttccgatgt gtcttcgaaa acgcaggatt tcagcatcgg
     5581 caacagttgt aatcccacca aacccgttcc ggcacctatt tcgaacgcct tccattgagg
     5641 ttcaatatca accagtttca tcagttcttc aacaaacttt tcggacatcc tgattttttc
     5701 gggttgatcc caatcgttgg ctttatgtgc aaaatgatca atcatgtttt gttattcatt
     5761 aagtgatgtt ttttaatttg caaaaataaa ctcttttgag aacaaaaagt tcaaatatag
     5821 gtgattattc gcacaataag ccgaaaagaa taaaaattgt atatttgcaa cgtgagagat
     5881 atgctgatgt gccaatttga tgatgtgccg atgtgacaat ttaaaaatgt ggagattggg
     5941 aatcagtgat gagtgatcag taattagtaa tcagtgatca gtgatcagag tcaggaacca
     6001 ggactattag agccaggagg caggacgaga agaatgatga atgattaacg gttaacggta
     6061 agtgattgct tcgacaatgc tcagcacaag tagtgattag tgatgtgctg atttgaagat
     6121 ttgaagattc taaattacac gtttctacaa ttcaacgatt acatactaca tactacacac
     6181 tacacattaa acattacata atcttgacaa actttacaaa ctcataaaac aatgaaatca
     6241 aattttaaaa caactcttat ggcaattgtc ctttcagccg tagtatcggc gtgtgacacc
     6301 aaaaaagatg aaggtccgat catcgaaaaa caggttcctg ttattgaaaa cggtctgatg
     6361 acacctgaag tgctttgggc attcggacgg gtaggcaatg tggaaatttc gcccgacgaa
     6421 acaaaaatcc tttacagtgt atcctattac agtgttgaac agaacaaaag caattcagaa
     6481 cttttcgtga tgaatgtgga tggttcggaa aaaaagcaaa taaccaaaac ggccagcaag
     6541 gagatcgctg ccaaatggat gaatgacagc aaacgcattg cttttttgtc gaacgaaagc
     6601 ggcagcatgc aactatggac tatgaatgac gatggaaccg gacgtaagca actctcagaa
     6661 cgcgaagggg atatcagtga tttcagcttt tcgcccgatg aaagcaaggt tctcctggtc
     6721 gccgatgtga aatacggaaa acgaacggtg gatatccatc ctgaccttcc gcaggctagc
     6781 ggagtcattg ttaatgacct gatgtacaaa cattgggatg aatgggtgca gactgtacct
     6841 catccgtttg ttgccagtgt ggaaggggga aaactaacga atgaaacgga cattctgaaa
     6901 gatgaaccgt atgaaagtcc gatgaaaccg ttcggaggta tcgaacagct tgcctggagc
     6961 cccgatggta aaacaatagc ttatacctgc cggaaaaaaa caggaaagga atacgcgctc
     7021 tcaacaaatt cggacattta tttctatgat ctggaaaccg gaaaatcggt caacaaaaca
     7081 gaaggcatga tgggatatga ccagaacccc gtgttttcgc ccgatggcaa gtggctggcc
     7141 tgggagagca tggaacatga aggatacgaa tcggacaaaa gccggctttt cgttatgaat
     7201 gtaaaaaccg gtgaaaagaa agacctaagt gctgactttg atcagaacag caacggactg
     7261 aaatggtcgg ctgacggtca atccatctat tttgtcagct gctttcacgc tgtaacacag
     7321 atatataaag cagacctgaa tgatggtaaa ataatgcctg ttacaaaggg agtacacgat
     7381 tatctgtcgg tgattccgct gaaaaatcaa ctgatcgggg ttaaacagtc gatgagtaaa
     7441 cccgacgaga tatatgccat caacgcggct accggtgaag aaaaagaact ctcttttgag
     7501 aataaggata tactcgacaa actggaaatg ggaaaagtgg aggaacgctg gattgcaact
     7561 actgacaaca agcagatgct cacctgggta atctatccgc ctcatttcga ctcagcaaag
     7621 aaatatccga ccctgctttt ctgtgaaggt ggtcctcaaa gtacggttag tcagttttgg
     7681 tcgtaccgct ggaattttca gatcatggcc gcaaatggat atattatcgt agctcccaat
     7741 cgtcgcggat tgcccgggtt tggttatgaa tggcttaaac aaatcagcgg tgactacggc
     7801 ggacagaata tgaatgatta tctctcggct atcgatgcag tagcaaagga acctttcgtg
     7861 gacaaagata aactgggttg tgtgggtgca agttacggag gcttttcagt ttactggctt
     7921 gccggtcatc acaacaaacg cttcaaagca tttatagctc acgatggcat gttcaacctg
     7981 ccgcagcaat atctggaaac ggaagagaaa tggtttgtta actgggacct cggcggcgct
     8041 tactgggata aaaacaatgc cgtggctcaa cggtcctatg cgaattcacc gcatcttttt
     8101 gtggataaat gggatactcc gattttgatt attcatggtg aaaaagacta ccgaattctg
     8161 gcttcgcagg gtatggctgc tttcgatgcc gccgtgctgc gtggtgtccc tgccgaaatg
     8221 ctgatatatc ccgatgaaaa ccactgggta ctgaaacccc aaaacggaat tctctggcaa
     8281 cgcacatact tcagctggct ggataaatgg ctgaaatgat gagtgatgag tgatgagtaa
     8341 tgagtaatga gtgatgagtg atgagtaatc agtaatcagt aatcagtaat cagtgaacag
     8401 taatcagtta ttaataacca gtgaacaatt atcattaata agttaagtta taaatatata
     8461 catgaaaaca catcatgatc ttacggtttg gaagaagtca attgattttg tgacgactgt
     8521 ctataaaata acagaagaat atcctaaaac cgaaatatat ggtctgacca atcaggttag
     8581 aagagcagtt gtctctgtac catccaatat tgcagagggt gcagccagaa catcaaaaaa
     8641 agaattttca cattttcttt caatttcgtt aggttcaata tcagaagtag aaacgcaatt
     8701 aattgtttca agaaatctga attatattac gaatgagcaa ttagaaaact tgctttcaga
     8761 actaatagaa attagaaaaa tgataattgg actgaaaaag acgatgagtg attactgatt
     8821 actgattact gattactgtt cactgattac ttaattcatg ttaaatttca actgcctctg
     8881 caacgttttg gggaaactaa tcatcatata ataaacaggg aataaattgg atggacgaac
     8941 aacgtttaat aaccggatgt aaacgtggcg aacaatgggc aaggagggag gtttatgaaa
     9001 aatatgcccc tgcaatgtta tccctgtgtg tacgttatgt agccgattat gaagctgccc
     9061 gcgatgtgtt gcaggatgga tttgtaaggg tgtttacaaa aacagatcaa tatgaaggga
     9121 gaggaaattt cgggggatgg atccggcagg tttttgttaa tacttccctc gaatatctga
     9181 gaagaaaaaa ctccctgcaa tttgaaacgc gggccgatga aatcaacgaa gcaaacgaac
     9241 ttcagaaccc ggaatccgga accctgactg ccgaagattt attggaatgt gtagctgaat
     9301 tgcctgataa actgagaaca gtatttaacc tgtatgccat agagggattt tcgcatggtg
     9361 aaattgccgc tatgacggga atacaggaaa gcacctcacg atcacatttc ttacgggcac
     9421 gactgctgtt gcaacagaaa gttactgaat tattgaataa tgaacatgca ggaaaaaaat
     9481 aaacatacga acgatcacga cgctttcagt caatgggcaa agcagcaact ggaaaatcat
     9541 cagctgccgg tggacgacga aatctggaag ggaattgaga aaaagcttgc tccggcaccg
     9601 aaaagaagtg ttcctttctg gtactggtta gcaggtgggg tggctgctgt ggttgcattg
     9661 atgttactgc tccgtccgtt ttatcattca tccggcagag atataaacac tcaaaccatt
     9721 gttagtcaga aaggcgcaaa taagcaagtt gttacttcag aacaaaatcc ggtagatatt
     9781 tccgtaagac cacaagtagt tgagaaagat catccggcaa ccggagtaaa atcaaataat
     9841 tctgccagac attcgaataa aaacatcttt attgcccgca attccgaaac ggtgaaaacg
     9901 aaacaaacgg aagaatttgg tggtatgact gcagaaaact tcgtatcaga catgcaaact
     9961 tccggtaatg agaaagaaaa aaaggaacgg acggtttcgg aagagcatcc tttgacaaca
    10021 aaagccggtg atgagggaaa aaataagaat gataattcaa aagcaaatgt ttcaaaacaa
    10081 cagattactt ctctgcccga tttatccgat tatccggaag aaccagccgt tgaaaccgtg
    10141 aaaaataaaa agaatgaaca ctggctttta gctgcttcac tgggagccgg aagcggaatt
    10201 agttccaacg gtagcggatt gttttttgaa acagggggat taatgagcga tgccgcgctt
    10261 gtcaacagca caagtgtaaa aaccgaatat acagcagata ttaaagacgc ggattttttc
    10321 aacgaagttc agcatcttcc tcctgtttcg ttcggactga tggctgaatt gcctttgaat
    10381 agaagttgga gcctggagag cggatttgta tacacatatc tggaaagccg gttgaaacga
    10441 acaaccaatc cgacagccac cgggcatctc aaacttcatt atttgggact tcctctgtac
    10501 ctgaaagcaa aactatggca tgatacgtcg tggagtattt acctgtcagc aggacccatg
    10561 atggaaaaag gattgaaatc aatttattca caggatattg attacacctg gtccatcacc
    10621 cacactgatg tgcattcgcg gattgaaggg ctgcaatggt cgcttaacac gggattggga
    10681 gtgaattata aattacagaa agatctgagt gtgtttttcg aaccaaaact gacttactac
    10741 cttgacaata atcagccaat gagcgcccgc acagagcaac cactggtgat tggactaaac
    10801 ggaggtttgc gtattgaatt atgaagtcaa tttgaagatt tgaagatgtg tcaatttgaa
    10861 gattttaaga tatgagaatg cgatgattta ttttaattca acacagacta tctgtaatga
    10921 cgggttcggt ggtttgctca ggttggtttc ccgcgcgatt tcctttgttg tgcaacttta
    10981 tatattctta tccgttatgt gcgaatcggg aaataaaaac tctatgagtt acgaagtcaa
    11041 cgattgagaa aagactattg aaagtcaaag atatacctga tcatagtcag gtttatataa
    11101 aaataagtaa cgcttacaat ggatttatcg tcaattaatt atatagaagt cgttttgaat
    11161 ttctgaataa aacaaaataa ataataccaa aatgagaaag ttgaatttgt tcgcaggaat
    11221 tattatctta agtgtattta ttggtagttg tacctacgaa gaagcagacc gtgagaaaat
    11281 agttgaaatg acaatatatc cggaaacagg atatggagca ggtgtactga gtgatgtggt
    11341 gacagagcca attcaatttt cggacaacga tgaaaatcga aagcagctgt tgacagatat
    11401 tattactgaa ggttttgatt tcaattatga aagaggttat gaatatactt taaaagtgaa
    11461 aaaggtatgg atgcaaaacc ctcctcaaga tgtttcttct gtcaaatata tatttattga
    11521 gttactcagc aataagaaag taataactac agatagcgaa aaatgcattg agctttttgt
    11581 gtcctcccaa acagtaaagt ttactccaaa gtatccgaat gaatatgaaa acgtaggagg
    11641 aatagaaacg ttgaaaattt ataatgctat acatgtacag gaaaagggta ctgacaattg
    11701 gatggcactt actaagatag agggttttga ttttgaaaaa ggctacgagt atgtaataaa
    11761 cgtaaaaaaa ataactcagt cagatccata ctcagtaaga tatgttttgc ttaatattgt
    11821 ttcgaagacg gaaaagaaat aaatgtacaa aacgctataa atataaaata actatgaata
    11881 aaaaagtaat tattattgcc tcattactgt tgctgttggt aactacaatc agcgcgcaga
    11941 cttcttatcc gaacaattat tccggtta
//
LOCUS       DAOPYB010000086        11871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6095_length_11871_cov_9.480366, whole genome shotgun sequence.
ACCESSION   DAOPYB010000086 DAOPYB010000000
VERSION     DAOPYB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11871
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6095_length_11871_cov_9.480366"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..724)
                     /locus_tag="PKH58_07800"
     CDS             complement(53..724)
                     /locus_tag="PKH58_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX88967.1"
                     /translation="MFDKILKIRIPVLILLLVCSVQGKAQGNLPYVDDKPYHFGYSLG
                     LNFMDFAVVPANIKNDVTVTAVSPGFSVGAISDMRLGRYFNLRFTPTLMLSERTLTYY
                     KDYDKVQVLSVPLYLPIYIKYSSERKDNYRPYILAGGGLWMDWGRNKERPVVLKTFDG
                     MLEAGIGCNLYFSFFKLSPELKFAIGLNNVITPTSMREGAVFGETDVISKLTTRMITL
                     SFNIE"
     gene            complement(855..3839)
                     /gene="infB"
                     /locus_tag="PKH58_07805"
     CDS             complement(855..3839)
                     /gene="infB"
                     /locus_tag="PKH58_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNX88968.1"
                     /translation="MSIRLSKACKDLNVGMATAVEFLAKKGHKIAADPNSKISDEEHL
                     LLAKEFNKDMALKLESERLSQERQAKEKAETVALEGFQKKPEKKEEMIPTTVSSDLRP
                     HIKEVGHIDLEKPKSAPQPSLKEQKPEPKAEHPEPIAEQPAPAPVQEPVAKEEKPVIE
                     PVKVETTTVPEPEKTEVKPQVPEKSEPEPVTEPTPAPEKRVIEITKKEPEKKVIETPK
                     PSVQKPTEEKNDEVESDDENNEEVFRLGKRTITAAPVVIGSIDLESLNQSTRPKSKTK
                     EQKKKEREERIKSHIDNRPKKAEPVKDAKAEGKTDTKNDAKTGKRKRINTQRVDINKP
                     ESLDFNKKQATKKEHFKKPIKTVVNEEDVQKQIKETLARLSNANKKGKGAKYRRDKRD
                     IVNAKLQEQAEREKSEESVLKLTEFVTVSELATMMNVSVNQVIGTCMSIGMMVSINQR
                     LDAETINIVADEFGFKTEYVSHDVIEALAASEESDDEANLVYRSPIVTVMGHVDHGKT
                     SLLDYIRKTNVIAGEAGGITQHIGAYNVKLDNGQRITFLDTPGHEAFTAMRARGAKIT
                     DIAIIIVAADDDVMPQTREAINHAVAANVPIVFAINKIDKPGANPEKIKETLANMNYL
                     VEEWGGKYQSQDISAKKGLGVKELLEKVLLEAEMLELKANPDRRAIGSIIESSLDKGR
                     GYIATVLVENGTLRQGDIVLAGINHGKIKAMFNERNQRLTEARPSEPVLILGLNGAPQ
                     AGDTFNVVESEQEAREIANKREQLQREQSIRTKKHFTLDELGRRIALGDFKELNVIVK
                     GDVDGSIEALSDSLLKLSTPHIQVNVIHKAVGAISDSDILLAAASNAIIVGFQVRPSA
                     SARKIAEKEEIDIRLYSIIYDAIEEIKSAMEGMLSPEIKEEVTATIEVLDVFKITKVG
                     TVAGCIVREGKVKRNNKIHLIRDGIVVFTGELDSLKRFKDDVKEVGVNFECGLNIKNY
                     NDIRVGDMIESFEEIEVKQTLN"
     gene            complement(3913..5166)
                     /gene="nusA"
                     /locus_tag="PKH58_07810"
     CDS             complement(3913..5166)
                     /gene="nusA"
                     /locus_tag="PKH58_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNX88969.1"
                     /translation="MSKKENINLIDTFSEFKELKSIDRSTLISVMEESFRNVISKTYG
                     TDENFDVIINPDKGDFEIYRNRTVVADDELTNENMEISLSEAHKIDGDYEVGEEVTDT
                     VDFFSFGRRAILTLRQTLASKILELQKDSLFVKYSEKVGEIVSAELYQIWKKEMLLMD
                     EDGNELYLPKTEQIPTDFYRKGETVRAVVAMVENKNNNPKIILSRTSPVFLQRLFELE
                     VPEIHDGLITIKKIARIPGERAKVAVESYDERIDPVGACVGVKGARIHGIVRELRNEN
                     IDVINYTNNVSLFIARALSPAKITSIRLNETDKKAEIYLKPEEVSQAIGKGGLNIKLA
                     SMLTGYTLDVFRDIDETENEEDIYLDEFSDEIDQWVIDALKAIGCDTAKSVLAVPRED
                     LIRRTDFEEETIDEVLAILAAEFEE"
     gene            complement(5170..5637)
                     /gene="rimP"
                     /locus_tag="PKH58_07815"
     CDS             complement(5170..5637)
                     /gene="rimP"
                     /locus_tag="PKH58_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HNX88970.1"
                     /translation="MILKDTVIQLVESFLANTEYYLVDLKISKDNSILVEIDSFRGVS
                     IDFCVELNRHIEANLDRETEDYELEVSSAGLTEPFKVLKQYEKNIGNEVEVFTKDNKK
                     MTGILISCDEIKIVLQQEKTVKPEGAKRKMTIQEDTEIPFTNIKTTKYIIRFK"
     gene            5825..6547
                     /locus_tag="PKH58_07820"
     CDS             5825..6547
                     /locus_tag="PKH58_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX88971.1"
                     /translation="MGRAFEYRKATKMKRWGNMARVFTKLGKQITIAVREGGSDIDTN
                     PRLRVLAQQAKKENMPKENVERAIKKATSRDEADYKEMVYEGYGPHGIAIVIETATDN
                     PTRTVGNIRSYFNRHGGSLGTTGSLEFMFDHKCVFHITKKEGVEIDDLELELIDFGVD
                     ELMEDEGEIILYGEFTSYSAIQKYLEENGYEITTAEFERIPKDLKEVTEEQRASINKL
                     IEKIEDDEDVQNVFHNMAEEEE"
     gene            complement(6638..7456)
                     /gene="panB"
                     /locus_tag="PKH58_07825"
     CDS             complement(6638..7456)
                     /gene="panB"
                     /locus_tag="PKH58_07825"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNX88972.1"
                     /translation="MSVHSEDTRKVTTFRLMEMKKRGEKISMLTAYDYTMASIVDQAG
                     TDVILVGDSASNVMCGNTTTLPMTLDQMIFMGKSVAKAVQRALVVVDMPFGSYQGNAD
                     IAVASAIRVMKETHADCIKLEGGKEIAESITRILTAGIPIMGHLGLMPQSINKYGSYT
                     VRAKEEAEAEKLISDAHFLEDAGCCSLVLEKIPAKLAGQVAKELRIPVIGIGAGGGVD
                     GQVLVVHDMLGLTKGFSPRFLRRYANLADVMNDAIASYVADVKSADFPNENEQY"
     gene            7685..8458
                     /locus_tag="PKH58_07830"
     CDS             7685..8458
                     /locus_tag="PKH58_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HNX88973.1"
                     /translation="MIKAVFFDIDGTLVSFETHRIPRSAREAIRQLQEKGIRVFVATG
                     RHESAINNLGDTRFDGYITINGGYCTVDDNICIYRNPIPKTDIEALIEYQKLNPFPCM
                     VMDEKGMSVNYHNRDVDHMLKMLDFPKIRVRPLREAINTDVLQLVSFFTADREDKIMQ
                     ILPHCESTRWYPLFTDIVPKGSSKAQGIDKIIEHYGISLSETMAFGDGGNDMTMLQHA
                     GTGVAMGNAEDKVKAVADFVTDTVDADGIVKALKKFGIF"
     gene            complement(8463..10649)
                     /locus_tag="PKH58_07835"
     CDS             complement(8463..10649)
                     /locus_tag="PKH58_07835"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX88974.1"
                     /translation="MRKYLIFLLAMLFFLSPASGQRKKVGVVLMGGGAKGFAHVGALK
                     VLEEAGIPIDYIAGTSMGAIVGGLYSIGYDSRTLDSLIHKQDWMHLLTDDVYRYNLTG
                     AEREKAQTYIVSINYAKQGIKLPSGIVDGQNILNLLLDLTTGYHDELNFRKLPIPFSC
                     VAADVKSGKEVVLNSGSLPVAMRSSMAIPGFFSPVKLDSMLLIDGGILNNYPVDVVRK
                     MGADIVIGISFEKDEKKVEKDMGSILELTHDFENFLGKEKFDQNLKNTDLPIRVNLGK
                     YSIASFQMDEIDSIMRIGEATTRKKWDELNELKKQLDISDNNLTPYIHNPYIGLDTLS
                     IRSVHVEGLSPEDEQMVLNRIRIQPKISRADLQNSISSLYGTNLFSKVHYRLENARPP
                     YDLVLEVEKKNIRNLNIGVRFDTEELASVLVNTSIRLKTTLNSTFGVTARLNRNPYLL
                     VDYSLNRSVLHKGSVKIKTSKNDIYIYDLGKLAYNIGYYQNSLKVNLSEFYFYNVKLQ
                     LGTTFDYFYYLYELRNSGQPSLNLESKPYINYSLNGTYDNLNSSYYATKGMYINFNYT
                     VTTNNFFRLDDQKPMQDLSFTLIKPVKLNKKMTLTPNVTGRIIVSDTVPVIYSNLVGG
                     QWSNHYVNQQISLEGLQGMEFFNKTVLKTQVNFKYDFNKNNSFQFVLNYTMHNDNPLE
                     LLKGRSYLGGTLGYTYSTLAGPIIFRLNYSDRTRKFYPFLSVGYYF"
     gene            complement(10767..11732)
                     /locus_tag="PKH58_07840"
     CDS             complement(10767..11732)
                     /locus_tag="PKH58_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNX88975.1"
                     /translation="MSEEIITDNHWLVITNPKAGKRKLPKQREYIVHTLNKAGIPFIY
                     KETEYSGHAIEIAKQYTRRGCSNFLVLGGDGSISEVINGIFAANTPDTSGIKLALIPR
                     GTGNDWGRFWKLKKNDKKSMSVFLQNNDQQIDIGKIEYRVDGIPETHYFINSVGFGLD
                     GEVCDLTHSLKKYTGSFSFLYTIALLAAIFRYKSTETEITVNNIKHKMKLFTMNIANG
                     CYSGGGIKQNPSALPYDGVFDMMMVEKPTFSDIITALPLIFNGKLTKHRVIKSLRTEQ
                     VDISCKENIMVEADGIIVPNAHSCKVSILKDAIRMIVPKSEMSRA"
     gene            complement(11749..>11871)
                     /locus_tag="PKH58_07845"
     CDS             complement(11749..>11871)
                     /locus_tag="PKH58_07845"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNX88976.1"
                     /translation="LNAVELEARGRHDPCVLPRAVPIVEAMTALTLVDLYLMNK"
BASE COUNT         3173 a         2465 c         2396 g         3837 t
ORIGIN      
        1 aatcatataa aatttaaaac cgacaaatct tgttttttgt cggtcagtag tgttattcta
       61 tattgaatga aagggtgatc attcgtgtgg tgagtttcga aatgacatct gtctcaccaa
      121 acacagcgcc ttccctcatg gatgttggtg tgattacatt attaagcccg atcgcgaatt
      181 ttaattcagg tgacaatttg aagaatgaaa aatacaaatt gcagcctata ccagcttcga
      241 gcataccgtc aaaggttttc agcactaccg gtctttcttt gtttctgccc cagtccatcc
      301 acaagccgcc tccggcaaga atgtacggac gataattatc cttacgttcg gaactgtact
      361 tgatatatat tggtaaataa agaggtaccg ataaaacctg caccttatca taatccttat
      421 aataagtgag tgtgcgctca ctcagcatca gggtaggagt gaagcgcaga ttgaaataac
      481 gacccagacg catatcgctg attgcaccta ctgaaaaccc cggagaaaca gctgtaacgg
      541 ttacgtcatt ttttatattg gcgggtacaa ctgcaaaatc cataaaattc agacccagtg
      601 aataaccaaa atgataaggt ttatcatcta cgtaaggtaa atttccctga gcttttccct
      661 gaacagaaca gactaataac aagataagaa cgggaattct tatttttaaa attttatcaa
      721 acatattcag atcatccatt tatgatttca aaacgttaaa acgtacaatt tattatcatg
      781 tttctgttta aaacaaacaa attttcatca actgatgatg aaaatttgtc tgaggtcatt
      841 attacggata tttgttagtt cagcgtttgt ttcacttcaa tttcttcgaa gctttcaatc
      901 atatcgccaa cccggatgtc gttgtaattc ttaatgttaa gaccacattc gaagttaact
      961 ccgacttctt taacatcatc cttaaagcgt ttaagcgaat ccagttcacc tgtaaatact
     1021 acaatgccat cgcggatgag gtgtatttta ttgttgcgtt taacttttcc ttcgcgaaca
     1081 atacaaccgg ctacggttcc aactttggtt attttgaata catcaagcac ctcgatagta
     1141 gctgtaactt cttccttgat ttccggggaa agcatacctt ccatggccga ttttatttcc
     1201 tcaatcgcat cgtagataat agaataaaga cgaatgtcta tttcctcttt ttctgctatt
     1261 tttcgtgctg atgcgctcgg gcgtacctgg aaaccaacga taattgcatt ggaagcagca
     1321 gcaagcaaga tatccgaatc tgaaatagct ccaactgctt tgtgaattac gttaacctgt
     1381 atatgaggtg tggatagttt aagcaatgaa tctgagagcg cttcaattga accatctacg
     1441 tcacctttca caataacgtt aagctccttg aaatcgccca gagcaatacg acgacccaac
     1501 tcatccagcg taaagtgttt ctttgtacgg atgctttgtt cgcgttgcag ttgttctcgc
     1561 ttattggcta tttcacgcgc ttcctgttcg ctttccacca cattgaaggt atctcctgct
     1621 tgcggagcgc cgtttaatcc caggataagc acaggttctg atggccttgc ttcagtgagg
     1681 cgctggtttc tttcattgaa catggctttg attttaccat gattaattcc ggcaagcact
     1741 atgtcaccct ggcggagagt tccgttttca accaaaacag tggcaatata accgcgtccc
     1801 ttatccagcg atgattcaat gatcgatccg atagcacgcc ggtcaggatt agctttcagt
     1861 tcaagcattt cggcttcaag aagcactttt tcgagcaact cttttacacc tagtcctttt
     1921 tttgccgaaa tatcctgcga ctgatatttt ccaccccatt cttccaccaa atagttcatg
     1981 ttggcaaggg tttcctttat tttctcagga ttagctcctg gtttatctat tttgttgatt
     2041 gcgaacacga taggaacgtt agctgctaca gcgtgattga tggcttcacg tgtttgaggc
     2101 atcacgtcgt cgtcggcagc tacaataatg attgcaatat ccgtaatttt ggcaccgcgg
     2161 gcacgcattg ctgtaaatgc ttcgtgaccc ggagtatcca gaaatgttat acgttgtccg
     2221 ttatcaagtt ttacattgta cgcgccgata tgctgtgtga taccgcctgc ttcacctgca
     2281 ataacgtttg ttttacggat atagtcgagc aatgaggttt taccatgatc cacgtgtccc
     2341 atcaccgtta caataggaga acggtaaacc agatttgctt catcatcgct ttcttcagaa
     2401 gctgcaagag cttcaattac atcgtgactc acatattctg tcttgaatcc aaattcatcg
     2461 gcaacgatat tgattgtttc tgcatccaaa cgctgattga tggataccat cataccgata
     2521 ctcatacagg taccgatgac ctggttgacc gaaacattca tcatagtggc caattcgcta
     2581 accgtaacga attcagtaag tttcaatacg ctttcttcac ttttttctct ttcggcctgt
     2641 tcctgaagtt tagcattcac aatatcccgt ttgtcacgac ggtattttgc acctttgccc
     2701 tttttattgg cattcgaaag ccgggcaaga gtttccttta tttgtttctg aacatcttct
     2761 tcattcacta ccgttttaat aggtttcttg aaatgttctt tttttgttgc ctgtttttta
     2821 ttgaaatcca gtgattcagg tttgttgata tcaacccgct gggtgtttat tctttttcgt
     2881 ttgccggttt tggcatcatt tttagtatcc gtttttcctt cagctttcgc atccttcaca
     2941 ggttcagcct ttttgggcct gttgtctatg tgacttttta tgcgttcttc acgctctttt
     3001 ttcttctgtt cttttgtctt ggattttggt ctggtactct gattcagact ttccagatca
     3061 attgatccga taacaacagg agcagcggtg atagtacgtt tcccaagtct gaaaacttcc
     3121 tcattatttt catcatccga ttctacctca tcatttttct cttctgttgg tttttgtacg
     3181 gatggttttg gagtttcaat aacttttttc tccggttctt tttttgttat ttcaataacc
     3241 cgtttttcag gagccggagt tggttcagtt acaggttcag gttcagattt ctcaggtacc
     3301 tgtggcttaa cttcagtctt ttcaggttca ggaactgtag ttgtttcgac ctttacgggt
     3361 tcaataacag gtttttcctc ctttgctaca ggctcctgta caggtgccgg agctggttgt
     3421 tctgctattg gttcggggtg ttctgctttt ggctcaggtt tttgttcttt taaagaaggc
     3481 tgaggtgctg attttggttt ttctaaatca atatgtccaa cttctttaat atgaggacgc
     3541 aaatcgcttg aaacagttgt gggaatcatc tcttccttct tttcgggttt cttctggaaa
     3601 ccttcaagag caactgtttc agctttttct tttgcctgac gttcctggct taggcgttcg
     3661 gattccaatt tcaaagccat gtctttgttg aactctttgg cgagtaacaa atgttcttca
     3721 tcgctgattt ttgaatttgg atcggcagct attttgtgtc cttttttggc aaggaattcc
     3781 actgctgtag ccattcctac atttaaatct ttacaagctt tacttaatct gatagacata
     3841 ttatttaatt acaatttttt tcaaaaataa ctttttccga aaaagcacat ttcggatagc
     3901 tttaatagac ttttattctt cgaattcggc ggctaatatt gccagcactt catcgatagt
     3961 ttcttcttca aagtcggtgc ggcgaatcag gtcttcgcgg ggtaccgcca gtacgctttt
     4021 tgccgtatcg caaccgatag ctttcagggc gtcaattacc cactggtcta tttcgtctga
     4081 gaattcatcc aggtaaatat cttcttcatt ttcggtttcg tcaatatcgc ggaatacatc
     4141 cagggtatat ccggtgagca tgcttgccag cttgatattc aaacctcctt taccaattgc
     4201 ctgagatact tcttcgggtt tcaggtaaat ttcagctttc ttatctgttt cgtttaagcg
     4261 gatagaagtg attttagcag gacttaatgc tcgggcaata aacagactta cattgttggt
     4321 gtaattaatc acatcaatgt tttcattgcg aagttcacga acgataccat gaatacgtgc
     4381 tccctttacg cccacacagg caccaaccgg atcaatgcgt tcatcgtatg attctacggc
     4441 tactttagct ctttcacccg gaatgcgggc aattttctta atggtaatca atccgtcatg
     4501 tatttcggga acttccagtt cgaacaaccg ttgcagaaat acgggcgacg tacgtgaaag
     4561 tatgatttta ggattgttgt ttttattttc aaccatggca acaacggcac gtaccgtttc
     4621 gcccttacgg taaaaatcag taggaatttg ctctgttttc ggcagatata gttcatttcc
     4681 gtcttcatcc atcagcaaca tctctttttt ccagatctga tacaattctg cactcacaat
     4741 ttcacccact ttttcgctgt acttaacgaa aagactgtct ttctgtaatt ccaggatttt
     4801 cgatgctaaa gtctgacgaa gtgtgagaat tgcgcgcctt ccaaaactga aaaaatctac
     4861 agtatcggta acctcttcac ctacttcata atcaccatct atcttatgag cttcggacag
     4921 gcttatttcc atgttttcat ttgtcagttc gtcatcagct acaacggtac ggttacgata
     4981 aatttcaaag tcgcccttgt ctgggttgat gattacgtca aaattttcgt ctgttccgta
     5041 ggttttagaa atcacgttgc gaaacgattc ttccataaca ctgataagtg tacttctgtc
     5101 gatgcttttc agttctttaa attcggagaa ggtatcaatt aaattgatgt tttctttttt
     5161 gctcatggct tatttgaatc tgataatgta ttttgttgtt ttaatatttg taaatggtat
     5221 ttcagtatct tcctgtatgg tcatcttgcg ttttgcacct tcgggtttta ctgttttttc
     5281 ctgttgcaaa actattttga tttcgtcgca actgattaaa ataccggtca tttttttatt
     5341 gtctttcgtg aaaacttcta cttcgtttcc gatatttttt tcgtattgtt tcagaacctt
     5401 gaaaggctca gtaagtccgg ctgaagacac ttccagttcg tagtcttctg tttcacggtc
     5461 gagatttgcc tcaatatggc ggttaagttc cacacaaaaa tcaattgaaa caccccggaa
     5521 tgagtcgatt tcaaccagga tgctgttatc tttcgatatt ttcaggtcaa ccagataata
     5581 ctccgtgtta gccagaaatg attcaactaa ttgtataaca gtgtctttta atatcattgc
     5641 tgttgatttt ataacaaaga aggggacttt ctgtccccct cacgttgttc aaattcgaat
     5701 gcaaaagtac gaataatttt ttaatttaca atagattatt gaaaatcatt gaaaaagatg
     5761 atgaattttc gtgcaatttt tctacttttg cagaccgaaa tttttaataa aacaatgcat
     5821 atttatggga agagcatttg aatatagaaa agcaaccaaa atgaaacgat ggggtaatat
     5881 ggcccgcgtt tttacaaaat taggaaaaca aattactatc gctgttcgtg aaggtggttc
     5941 agacattgat accaatccac ggttaagggt tttagctcag caggcaaaaa aggaaaatat
     6001 gcccaaggag aatgttgaaa gagctatcaa aaaggctact tcgagggatg aagctgacta
     6061 caaggaaatg gtgtatgaag gttacggacc tcatggtatt gccatcgtga ttgaaacggc
     6121 tacagataat ccgacccgta cagtgggaaa tatccgcagt tatttcaacc gtcacggcgg
     6181 ttcactggga acaaccggtt cactggagtt catgtttgac cacaaatgtg tatttcatat
     6241 tacaaaaaaa gaaggtgttg aaattgatga tctggaactg gaactaatcg atttcggcgt
     6301 ggatgaattg atggaagacg aaggtgaaat aattctttac ggcgaattca cctcatattc
     6361 ggccattcag aaatacctgg aagaaaacgg atacgaaatt acaactgccg aatttgaacg
     6421 gattccaaag gatctgaaag aagtgaccga agaacaacgt gcatccatca acaaactgat
     6481 tgaaaaaatt gaagacgatg aggatgttca gaacgttttt cacaatatgg ccgaagaaga
     6541 agaataatcg aaattgcaga tattaacaaa aaaagaacaa ctcaaacggt tgttcttttt
     6601 ttgttacaat atgttttact gcagcggatt tccggtatca gtattgttcg ttttcgttcg
     6661 gaaaatcagc tgatttcaca tcggctacat agctggcaat ggcatcattc atcacgtcgg
     6721 ccaggttggc atagcggcga aggaaccggg gtgagaaacc tttggtcaga cccagcatat
     6781 cgtgtacaac gagtacttgt ccgtcaacac ctccacctgc accaattcct attacaggaa
     6841 ttctgagttc tttagcaacc tgcccggcta gttttgccgg aattttttcg agaacgagcg
     6901 aacagcatcc tgcatcttcc agaaaatgag catcactgat cagtttttct gcttccgctt
     6961 cttcttttgc ccgtacggtg taggaaccat atttattgat ggattgaggc atcagtccca
     7021 gatgtcccat gatgggaata ccggcagtga gtatgcgggt gatagattct gcaatttctt
     7081 ttcctccctc cagcttgatg cagtcggcat gagtttcctt catcacacgg atggcagaag
     7141 ccactgcaat atcagcatta ccctgataac tgccaaaagg catatcgaca accaccaaag
     7201 ctctctgtac agcttttgct accgatttac ccataaaaat catctgatcc agtgtcatcg
     7261 ggagcgttgt ggtgttgcca cacatgacgt tggaagcaga atcgccaacc aggatgacat
     7321 ccgttccggc ctgatcaacg attgaagcca tggtataatc gtaggcggta agcatactga
     7381 tcttttcgcc tctctttttc atttccatca ggcggaaggt tgttactttt cgggtatctt
     7441 cactgtgtac tgacataatt tatacatttt tgttgattaa acgggttgca aagttacaaa
     7501 taataattat aaactgttct gtagttatgt ttaataaaga tttataattc gctgaagggt
     7561 tttcaggttt ttgtgtaatt ttgtgcttaa actaaaagta attaacaggt agcatttgtt
     7621 gtaaatgtga tgtttgattg tcaaatgtcc taattttatt gtgtgattaa aaatatgaca
     7681 gtatatgata aaagcagtat tttttgatat agatggaaca ctggttagtt ttgaaactca
     7741 ccggataccg cgttctgcca gagaagcgat ccggcaactg caggaaaaag gcatcagggt
     7801 gtttgtggct accggtcgtc acgagtctgc gatcaataac ctgggcgata cacggtttga
     7861 cggttatatc accatcaacg gcggatactg cacggtagat gataatatct gcatttacag
     7921 aaaccctatc ccaaaaactg atatcgaagc gctcattgag tatcagaaac tcaatccgtt
     7981 tccctgtatg gtaatggatg agaagggaat gtcagtcaat tatcacaacc gcgatgtgga
     8041 tcatatgctg aaaatgctcg attttccgaa aatcagagtt cgtccgctcc gtgaagctat
     8101 caataccgat gttttgcagt tggtttcatt ttttaccgca gacagggaag acaaaattat
     8161 gcagatttta cctcactgcg aatctacccg ctggtatccg ctgtttaccg atattgttcc
     8221 gaaagggagc agtaaggcac agggcataga taaaatcata gaacattatg gcatatcgct
     8281 cagtgaaacg atggcatttg gcgatggcgg gaatgatatg accatgctgc aacatgccgg
     8341 aacaggcgta gccatgggaa atgccgaaga taaagtgaaa gccgtggcag atttcgtgac
     8401 cgatactgtg gatgccgacg ggattgtaaa agcgctgaag aaattcggaa tattctgacg
     8461 gactaaaaat agtaacctac actcaggaaa ggataaaatt ttctagtccg gtccgaatag
     8521 ttcagtctga aaataatagg accggcaagg gtgctgtatg tatatcccag tgttcctcct
     8581 aagtatgacc tccctttcag taattccagc ggattatcgt tatgcatggt atagttcaga
     8641 acaaactgaa atgagttgtt tttattgaag tcatatttga aattcacttg cgttttcaat
     8701 accgttttgt tgaaaaattc cattccctgt aaaccttcga gcgaaatttg ctgatttaca
     8761 tagtgattac tccactgtcc gcctaccaga ttggaatata ttacaggaac cgtatcgctg
     8821 acaatgattc ttccggtaac attgggagtt aatgtcattt ttttgttcag tttcacaggt
     8881 ttaataaggg tgaaactcaa gtcctgcatg ggtttctgat catccagacg gaaaaaattg
     8941 ttggtggtaa ccgtataatt gaagttgata tacatacctt ttgtagcgta gtaactactg
     9001 ttcaggttgt catatgtgcc attcagcgaa tagtttatat aaggttttga ttcgagattc
     9061 aaagatggct gacctgaatt ccggagttca tagaggtaat aaaaataatc gaaagtggta
     9121 ccaagctgaa gtttgacatt gtagaaatag aactcactca aatttacttt caggctattc
     9181 tgatagtatc cgatattgta tgccagtttt cccaggtcgt aaatgtaaat gtcgttttta
     9241 gaagttttaa tttttacact tcccttgtgc agcacgctgc gattaagcga ataatcgacc
     9301 aacagatacg gatttcggtt caggcgggca gtaaccccga atgttgaatt tagtgtggtt
     9361 ttcaacctga tagaggtgtt taccaaaaca gatgccaatt cttcggtatc aaaccgaaca
     9421 ccaatgttca aattccggat atttttcttc tcaacctcaa gtaccagatc gtagggtggt
     9481 ctggcgtttt cgagcctgta atgaaccttc gaaaataaat tggtaccata taggcttgaa
     9541 atactgtttt gcagatcagc ccgtgatatt ttcggttgaa tccgaatgcg gtttaatacc
     9601 atctgttcgt cttcgggtga taatccctcg acatgtacac tccttatgga taaagtatcc
     9661 agcccgatat atggattatg aatgtacggg gtcaggttat tgtcggatat atcaagttgt
     9721 ttttttagtt cgttcagttc atcccatttt tttcgtgtcg ttgcctcacc aatacgcatt
     9781 atgctatcaa tttcgtccat ctggaagctg gcaatagaat atttccccag attaacccgg
     9841 ataggcaggt cggtattttt aaggttttga tcaaattttt cttttcccag aaaattttcg
     9901 aaatcgtgag ttaattccag tatcgaaccc atgtctttct ccactttttt ttcgtccttc
     9961 tcaaagctga tgccgataac aatgtcggct cccattttcc ggaccacatc cacaggatag
    10021 ttattgagaa ttccgccatc aatgagtagc atgctatcca gttttacggg actgaagaaa
    10081 cccggtattg ccatgctgga gcgcattgca acgggcagcg atccgctgtt gagtacaact
    10141 tcctttcccg atttgacatc agctgccaca cagctgaaag ggattggtag tttcctgaaa
    10201 ttcagttcat cgtgataacc tgtggtcagg tcgagcagca ggtttaatat attctgacca
    10261 tcaacaattc ctgaaggcag tttaatgccc tgtttcgcat agttgatgga aacaatataa
    10321 gtttgagctt tctctctttc cgcccccgtc aggttataac ggtaaacatc gtcggtgagc
    10381 agatgcatcc agtcctgctt atgtatcagc gaatccagcg tccgtgaatc ataaccgatg
    10441 ctgtataaac ctcccacaat agctcccatg ctggtgccag caatgtaatc gataggtatt
    10501 cctgcttctt cgagaacttt cagcgcacct acgtgtgcaa accctttagc tccgccaccc
    10561 atcagaacga cgccgacttt tttccgctgt cccgatgcgg gtgaaaggaa aaacaacatc
    10621 gcgagaagaa aaatcaggta tttgcgcata ggttttaaaa caatttttac ttgatgtaag
    10681 tgaatttaca atatataact actaatcagt tgttttgttt cacagtcatt cctatttccg
    10741 gtgaaatttt taacggattt tcggatttat gctctcgaca tttcactttt gggtacaatc
    10801 atccggatgg cgtctttcaa aatgctgact ttgcagctgt gagcattcgg aacgattatt
    10861 ccatcagcct caaccattat gttttccttg caactgatat caacctgctc cgtgcgcaat
    10921 gattttatca cacggtgttt ggtgagtttt ccattgaaaa tcaacggcaa tgcggtaata
    10981 atatctgaaa aagtaggttt ctccaccatc atcatatcaa aaacaccatc gtagggcagg
    11041 gcagaagggt tttgtttgat accgccacca ctgtagcatc cattggctat gttcatggtg
    11101 aaaagcttca ttttgtgttt tatattgttg acagtgattt cagtttcggt cgatttatat
    11161 ctgaaaatgg ccgccagcag ggcaatggta taaagaaacg aaaaacttcc ggtgtatttt
    11221 ttaaggctgt gagtgaggtc acagacttca ccatcgagtc cgaacccgac ggagttgata
    11281 aaataatgcg tttcggggat gccatcaacc cgatattcaa tttttccgat gtcaatctgc
    11341 tgatcgttat tttgcagaaa gacagacatg gatttcttat cgtttttctt cagtttccag
    11401 aatctgcccc agtcatttcc ggtaccacgc ggaattaaag caagtttaat tcccgaagta
    11461 tccggggtgt ttgcagcaaa gatgccgttg ataacttcgc ttattgatcc atcgcccccc
    11521 agcaccagaa agttgctgca tccgcgacgg gtatattgtt ttgcaatttc aatggcatgt
    11581 cctgaatatt cggtttcttt gtagatgaaa ggaattccgg ctttattcag tgtgtgaacg
    11641 atgtattcgc gttgttttgg taatttcctt ttaccggctt tagggttggt gatgaccagc
    11701 caatgattat cggtaataat ttcttcggac acggtggtat agataaagtt acttattcat
    11761 taaatataaa tcaacgagag taagagcagt catagcttcc actatgggca cagcgcgtgg
    11821 caagacacac gggtcgtgac gtccgcgggc ttccagctct acagcattca g
//
LOCUS       DAOPYB010000087        11866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6108_length_11866_cov_8.514351, whole genome shotgun sequence.
ACCESSION   DAOPYB010000087 DAOPYB010000000
VERSION     DAOPYB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11866
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6108_length_11866_cov_8.514351"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKH58_07850"
     CDS             complement(<1..238)
                     /locus_tag="PKH58_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88977.1"
                     /translation="MKKALFISIIKRSIVLPFLLLAFVANSQTYPFQNPNLSSDERAK
                     DLISRLTLEEKATLMCDQSDAIPRLGIKKFNWWSE"
     gene            complement(459..2219)
                     /locus_tag="PKH58_07855"
     CDS             complement(459..2219)
                     /locus_tag="PKH58_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNX88978.1"
                     /translation="MKKNTIGWSLFSRRQIVLLTVYISVFSVAAIAGNTTKKTDEIKN
                     APVFSKFVYQGNDRVYNENPLKADEFYNPILQGCYPDPSITRKGSDYYLVCSSFAMFP
                     GVPVFHSKDLVNWTQVGHVLDRKSQLKVQDTGISAGIYAPTIRYNPYNDTFYMITTQF
                     AGGMGNIIVKTKDPFQGWSDPIQLKFDGIDPSIFFDEDGKAYVVHNDAPDKGKELYTG
                     HRVIKVWEYDVEKDQIIPGTDKIIVDGGVDLSKKPIWIEAPHLYKKNGKYYLMCAEGG
                     TGDWHSEVIFVSDSPKGPFKPAPGNPILTQRYFSNDRSYKVDWAGHADLIDGPDGKYY
                     GVFLAVRPNEKQRVNTGRETFILPVDWSGTFPVFENGLIPLEPKLKMPAGVENKTGKE
                     GFFPNGNFTFTDDFTEPKLDFRWIGLRGPREDFISITKKGLKITPFQVNIKEMKPTST
                     LFYRQQHIIFSFNATMEYTPKSEKELAGITCMQSEKFNYVFGITKKGKDFYLLLERTE
                     KGNSTILASTKIDVKNPVGLQVKAVGDNYEFSYSLNGTDFVNLGGTVSGDILSTNVAG
                     GFTGALIGLYATAANDAKPE"
     gene            complement(2266..3144)
                     /locus_tag="PKH58_07860"
     CDS             complement(2266..3144)
                     /locus_tag="PKH58_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX88979.1"
                     /translation="MKNNILSLIVALLIIQTANGQSHKAAPDGFDIPKTGIEHGRIDT
                     ITYHSETVANDRRALIYTPPGYSKTRKYPVLYLLHGIGGDEKEWFKQGNPQVILDNLY
                     AEKKIVPMIVVLPNGRAMKNDRAEGNIFDAQKVEAFATFEKDLLNDLIPFVEKNYPVR
                     KNQKNRALAGLSMGGGQSLNFGLGNLNTFAWVGGFSSAPNTKIPQELMPDVKYAKKKL
                     KLLWISCGDKDGLITFSKRTHDYLIANQIPHIYQVIPGGFHDFKVWKQNLYMFSQLLF
                     KPVTNSLIEQYSNIPE"
     gene            complement(3248..5185)
                     /locus_tag="PKH58_07865"
     CDS             complement(3248..5185)
                     /locus_tag="PKH58_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNX88980.1"
                     /translation="MKVLSFRNITVFLLLFLAGSAVAEVRLPKLISDGMVLQRDAKVK
                     IWGWASPSEKVTVQIAAQTLTCKANEAGEWTVQLPPQKAGGPFTMEIKGENTVKISNI
                     LFGDVWLCSGQSNMELPIYRVKPLYEEEIKIAQNTNIRYFAVPQKYNLKSAENDYVSG
                     SWQELNPKSILNFSAVCYFFGKELFQKYNVPIGLINASLGGSPIQAWMSEEALAAFPQ
                     YLNDAIKYRDDQLIRETEAAENKKVNDWYAEANKKDAGQAAGWKLPSFDDTDWQKMNV
                     PGYWSETPIGKVNGVVWFRREFDIAKENAGKAAFLNLGRIVDADSVFVNGKCVGNITY
                     QYPPRWYNIPENLLVEGRNVIAVRIVSNSGRGGFVPDKPYELKVANSVIDLKGVWKMK
                     LGCSMPPTPGTTFFRWKPMGLYNGMIAPLVNYNKKGVIWYQGESNTAIPQEYATLLPT
                     MISSWRKNFNQKTLPFVYAQLPNFMETKTQPTESNWAVFRDMQTKTLSVPGTAMTVNV
                     DLGDWNDIHPMNKKDVSHRLVLAAQKVAYKDRKVVYSGPLYKSMKVKGNKVDLSFSNT
                     GSGLIAKDGKELRYFAIAGADKKFVWAKAEIIGKKVIVWSDDITNPVAVRYAWSDNPE
                     GANLYNKEGLPASPFNAELNK"
     gene            complement(5205..6176)
                     /locus_tag="PKH58_07870"
     CDS             complement(5205..6176)
                     /locus_tag="PKH58_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNX88981.1"
                     /translation="MKQPRYLVPNLYTADPAVHIFNGKIYIYPSHDVESGIPENDNGD
                     HFDMRDYHVFSMESIDGEVTDHGVVLDVQSIPWAGRQLWDCDAAYKDGKYYIYFSLKD
                     KNDVFHIGIAISEKPEGPFVALDSPIKGSYSIDPCVYREDDDSHFMCFGGLWGGQLQR
                     YRNNKAIECGHEPADNEPALYAKIVKMSENMLDFAEEPRDLVILDENGEPLRAGDHDR
                     RFFEASWMHKYNGKYYFSYSTGDTHLLCYALSENIYGPYTYQGVILTPVVGWTTHHCI
                     TEFEGKWYLFHHDCVPSGGKTWLRSLKVVELEYNADGTIKTIEGLAK"
     gene            complement(6223..7365)
                     /locus_tag="PKH58_07875"
     CDS             complement(6223..7365)
                     /locus_tag="PKH58_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNX88982.1"
                     /translation="MKKSLNILTLCLVVFVATFSCSAKLTMNEPATLKDALADKFYIG
                     TAMNTGQINGTDTASLRVMKEQFSAVVAENCMKSEVIQPEEGKFDFTLADKFVDFAEK
                     NKLYATGHVLIWHSQAPKWFFVDADGKDVSPEVLKQRMIKHISTLVGRYKGRIKGWDV
                     VNEAFEDDGSWRKSKFYEILGEDYIKLAFEYAHDADPDAELYYNDYSMAHPGRRDAVV
                     KMVNNLKQQGIKVDGIGMQGHMTMNFPTCDAYEKSILAFASTGAKVMITEMDMTVLPP
                     PESYQGADVGKNYQFDSKMNPYPNGLPDSVATALHDRYAAFFRLFLKHSDKISRVTVW
                     GVSDAQSWRNNWPMRGRTDYPLLFDRKLRPKPIVKTIISQANAMKK"
     gene            complement(7407..8894)
                     /locus_tag="PKH58_07880"
     CDS             complement(7407..8894)
                     /locus_tag="PKH58_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX88983.1"
                     /translation="MNSKSQKVSFGEKVGYSLGDGSANLIFQMMMMFQLFFYTDVFGI
                     KATAAGMILLVARLFDAFVDPMVGILSDRTNTRWGKYRPWLLWTAVPFAVFFILAFST
                     PDLGERAKILYAGVTYILLMSIYSFNNTPYSSLGGVMTSDIKERTSISSIRFVTATIA
                     TFVVQGLTLPLVSKFGQGDAQKGWSSTILLFAVVVVVLMLITFLTTKERISPPVNQKT
                     SIKQDFKDIISNKPWKAMFALTLFLFITLSMWGSSMSYYFNYVVDKDALFSFLRNFGL
                     VSDAGQSYGVMHGFLNAFGLIASDNSQVFAVGFSLFNMIGQIITLLGVIFLSGWLSGI
                     FGKRNVFVVCLALTAVFTSLFFVVPSDNITLIFIINILKSLAYAPTIPLLWAMMGDVA
                     DHSEWVNHRRATGFVFAGIVFALKAGLGVGGAICGAIIDAFGFIPNTIQTADSIFGIK
                     LTSSLIPAATFTIGVIALLFYPISKSFNEKIQAELSSRRAEDNLK"
     gene            complement(8942..9235)
                     /locus_tag="PKH58_07885"
     CDS             complement(8942..9235)
                     /locus_tag="PKH58_07885"
                     /inference="COORDINATES: protein motif:HMM:NF036499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88984.1"
                     /translation="MLESPTLNFNDSKGLRYGISFNDGTEQIVNVNEKYDIKKMEKWQ
                     ANSINETITCHKIDNAGKHTLRFRVLEPGIVLQKKMIDLGGLKKSYLGAPERY"
     gene            complement(9732..11699)
                     /locus_tag="PKH58_07890"
     CDS             complement(9732..11699)
                     /locus_tag="PKH58_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX88985.1"
                     /translation="MKKINFKVLMGAMMMLFLSACNDILDEQPRATYEPGFFTTKEGV
                     QSGLTSMYAHLRDIYGQGYYYNITLTGTDEYTWGNSADGNFKDADMTSGGTMTPSTSR
                     ADVLWNAAFPNINTASGVIENAEAVGLAPSLIAEARFFRAFDYFQLVQTFGGVPLDLG
                     GGVLKFNKSTVRTSVRNTVPEVYTIGIFPDLKQAITDLPENPRIVGAATKTLARLFLA
                     KAYLTYGWWLENPNGIPTYPQAARTDPDGHNAAWYFQQAYDVATEAITSPGYAGLQSS
                     YYKVHLAQNDRNNEILLLADHTNASEQYNGGVSFSYGSGAAPDNFACWMVTPQMENIR
                     SAKKSDGTDLVSSVQREAAQWGGRPWTRMAPPQGVFTKTFADKTNDSRFDGTFTSVYR
                     GNWNKNASMSSITTLYNANNMPVSPGDAILSFLPDESLTSTLSYTVGKSNIGAGVVAG
                     RADYVIPPSGINRQYFPGLWKIGTYRTDNGSGLGTPNGSTCRPFVIAKFSELFLVAAE
                     AAVKGATTKAVTGTYANDGTARGLVNVLRARAGKWSFSNAENKAKVEDHSAEMIAATP
                     ATIDINYILAERSREFFGEGYRWFDLVRTQKWEEIAGTYEIADTGTHTPKTFTRVINK
                     AVHYLRPIPQAQIDRLDMSDDEKKAYQNPGY"
BASE COUNT         3433 a         2575 c         2191 g         3667 t
ORIGIN      
        1 cttcgctcca ccaattgaat tttttgatgc ccaaacgtgg tatagcatcc gactgatcgc
       61 acatcaacgt ggctttttct tccagcgtta atctggatat aagatctttt gcccgttcat
      121 ccgaactcag atttggattc tgaaacggat aggtttggct gttggccaca aaagccaata
      181 acaaaaacgg aagaacaatt gatcttttta tgatcgaaat aaataaggct ttcttcatgt
      241 aaaaaataga gtttatataa gtatgatatt tatcgaatat tattctgtta aacgcaaagt
      301 tattaagatg caaaaaaaaa cttttaagtt ttttactttg cgctctttgc gaatatttga
      361 ttatatgata ataggcatat ttgagctttg cgttaactat taaatcatca agagatgcac
      421 taacttatat aatgatttca atcaaaaaaa atgaaaagtt attccggttt tgcatcgttt
      481 gccgctgtgg catacaatcc aatcaaagct cctgtaaatc caccagccac attggttgat
      541 aatatatcgc ccgaaactgt acctccaaga tttacaaagt cggttccatt gagcgaataa
      601 ctaaactcat agttatcacc aactgctttt acctgtaaac caaccggatt tttcacgtca
      661 atctttgtac tggctaaaat agtagaatta cctttctccg ttctttcgag caataaatag
      721 aaatcctttc ccttttttgt tattccaaaa acataattga atttttcgct ttgcatacag
      781 gtaattccgg ccagttcctt ttcggatttt ggggtatatt ccatcgtagc attaaatgag
      841 aaaattatat gttgttgtcg gtaaaaaagg gtggatgttg gtttcatctc cttgatatta
      901 acctgaaaag gagtaatttt caatcccttt ttagttattg aaataaaatc ttcccgcgga
      961 cctcttagtc cgatccatct gaaatccagt tttggttcag taaagtcatc ggtaaacgta
     1021 aaatttccat tcggaaaaaa tccctctttc cctgttttgt tttcaacgcc tgccggcatt
     1081 ttgagtttcg gttccagggg tataaggccg ttttcaaaga caggaaatgt gccactccaa
     1141 tccacaggca gaataaaagt ctcacgacca gtatttacac gttgcttttc gttggggcga
     1201 acagccagaa aaacgccata atacttacca tcaggaccat caatcaagtc ggcatgtccg
     1261 gcccaatcaa ctttgtagct tctgtcgtta gaaaaatacc gctgcgtaag tatcggattg
     1321 cctggagcag gtttgaaagg acctttggga ctatcgctca caaaaatcac ttcactgtgc
     1381 caatcaccgg ttcctccttc ggcacacatt aaataatact ttccattttt tttatacagg
     1441 tgaggcgctt caatccagat tggttttttc gaaagatcaa cgccgccatc tacaataatt
     1501 ttatctgttc cgggaattat ctgatctttt tccacatcat attcccaaac tttaatcaca
     1561 cggtgtccgg tgtacagctc ttttcctttg tcgggtgcat cgttatgaac aacatacgct
     1621 ttcccgtctt cgtcgaagaa gatggaaggg tcaatgccat caaatttcag ttgaatggga
     1681 tcgctccaac cctgaaacgg atcttttgtt tttacaatta tatttcccat tccgcctgca
     1741 aactgagtgg taatcatgta aaaagtatcg ttgtacggat tatagcggat ggttggtgcg
     1801 taaatgccgg cgctaattcc ggtatcctgc actttcagtt gcgatttccg atcgagcaca
     1861 tgacctacct gtgtccagtt caccaaatct ttggaatgaa aaacaggtac gccgggaaac
     1921 atggcaaatg aggaacatac caaataataa tcactacctt tccgtgtaat gctgggatcg
     1981 ggataacaac cctgcaatat aggattatag aattcatcag ccttcaacgg attttcatta
     2041 tacaccctgt cattgccctg ataaacaaat ttgctgaaaa caggagcatt tttaatttca
     2101 tccgtttttt ttgtcgtatt accggcaatt gcagcaactg aaaatacgga tatatagact
     2161 gttaacaata cgatttgtct tctgctaaat aaactccatc caattgtgtt ttttttcatc
     2221 tttttctttt ttaattatct aaaatctttg ggttgaaata agtctctatt caggtatatt
     2281 gctgtattgt tcaatgagtg aattagtaac cggtttgaat aaaagctgtg agaacatata
     2341 caggttttgc ttccagactt taaaatcgtg aaatccaccg ggaataacct gataaatatg
     2401 tggaatctga ttagcaatca gataatcgtg cgtgcgtttg ctgaaagtaa tcaacccgtc
     2461 tttgtcgcca cacgaaatcc agagtagttt cagctttttc ttcgcgtact ttacatccgg
     2521 cattaattcc tgaggtattt tggtgtttgg agccgatgaa aaacctccga cccaggcaaa
     2581 agtattcagg ttgcccagtc caaaattcaa tgactgtcca ccacccatcg aaagtccggc
     2641 caaagcgcgg tttttctgat tcttgcgtac cggatagttt ttttcaacaa aaggaataag
     2701 gtcattgagt aaatcttttt cgaacgttgc aaatgcctca actttttgag catcaaatat
     2761 attgccttct gccctgtcat tcttcatggc tcttccattt ggcagcacta caatcatggg
     2821 tacgattttc ttttcagcat acaggttatc tagtatcacc tggggatttc cttgcttgaa
     2881 ccattctttt tcgtcgccac caataccatg aagcaggtaa agcaccggat attttctcgt
     2941 ttttgaatat ccgggaggtg tataaatcaa tgctctgcgg tcattagcaa ccgtttcgga
     3001 atgatatgta attgtatcaa tcctgccgtg ttcaattccg gttttcggaa tatcaaatcc
     3061 gtccggcgca gccttatgag actgaccatt tgcagtttgg attattaata atgctacaat
     3121 taatgataat atgttgtttt tcatcgaatt aataaatatt ttcaaataaa aggacatcaa
     3181 ctacatttac tgataatatt cataaacgca agttggttaa taacatctga caaaccaaaa
     3241 taaatttcta tttatttaat tccgcattga atggtgatgc cggtaaacct tccttattat
     3301 acaaattcgc tccttcggga ttatcggacc aggcataacg tactgcaacg gggttggtta
     3361 tatcatcgct ccaaacaatc acttttttgc caataatttc ggcttttgcc caaacaaact
     3421 ttttgtcggc acctgcaatg gcaaaatatc tgagttcttt tccatctttg gcaataagtc
     3481 cgcttcccgt atttgaaaaa ctaagatcta ccttatttcc ttttactttc atggatttat
     3541 acaaaggtcc cgaataaacg acctttcggt ccttgtacgc aaccttttga gcagccaaaa
     3601 ccaatcggtg tgatacatct tttttgttca taggatgaat atcattccaa tcacccaaat
     3661 cgacattaac tgtcatagca gtgccaggaa ccgataatgt ttttgtttgc atatctctga
     3721 aaacagccca attgctttca gtcggctgag ttttcgtttc cataaaattt ggcaattgtg
     3781 catacacaaa cggtaaagtc ttttgattga aattcttgcg ccaactggaa atcattgttg
     3841 gtaaaagggt ggcatactct tgcggaatag ctgtgtttga ttcgccctga taccaaatta
     3901 ctcctttttt attgtaattg accaacggtg ctatcatgcc attgtacaaa cccattggtt
     3961 tccaacgaaa aaaagtagtt cccggcgtag gaggcattga gcagcctaat ttcattttcc
     4021 agaccccttt taaatcaatt actgaatttg ccactttcag ttcataaggt ttatccggaa
     4081 caaaaccgcc tcttcctgag ttacttacaa ttcgaacagc aataacattt cgtccttcaa
     4141 ccaacagatt ttcaggaata ttataccaac gaggcggata ttggtatgtt atattgccca
     4201 cgcactttcc gtttacaaaa actgaatcag cgtccacaat tcgccctaaa ttcagaaaag
     4261 ctgcttttcc ggcattttct ttcgcaatat cgaattctcg tcgaaaccaa accacaccat
     4321 ttacttttcc aatgggagtt tcgctccaat aaccgggaac attcattttc tgccagtcag
     4381 tatcatcaaa cgaaggaagt ttccaacccg ctgcttgtcc tgcatctttt ttatttgctt
     4441 cggcatacca atcatttact tttttgtttt cggcagcttc cgtttctcga atcaattgat
     4501 catcacggta tttaatagca tcattcagat actgtggaaa agcagcaagt gcttcttcgc
     4561 tcatccaggc ctgtataggt gaaccaccca aactcgcatt aatcagtccg attggaacat
     4621 tgtatttttg aaataattct tttccaaaaa aataacaaac tgccgaaaaa ttcaaaatac
     4681 tttttggatt taattcctgc caacttcctg aaacataatc attttctgct gatttcagat
     4741 tatatttctg aggaacagca aaatatcgaa tatttgtatt ttgggcaatt ttgatttcct
     4801 cttcataaag cggtttgacc ctatagatcg gtaattccat attggattgc ccggaacata
     4861 gccaaacatc gccaaagaga atattgctta ttttgaccgt gttttcacct tttatttcca
     4921 tcgtaaaagg tccaccggct ttctgaggag gaagttgtac agtccattcg cctgcttcgt
     4981 ttgctttgca agtcagtgtc tgagcggcaa tttgcaccgt tactttttca gagggtgatg
     5041 cccaacccca tattttcact tttgcatcgc gttgcagaac cataccgtct gaaatcagct
     5101 ttggcaaccg gacttcagct actgcagaac ctgcaagaaa aagaagaaga aatacagtaa
     5161 tatttctgaa tgataatact ttcatttact ctgccagtct ttatttattt tgctaaacct
     5221 tcgatggttt taatcgttcc atcagcattg tattccaact ccaccacttt caggcttcgc
     5281 aaccatgttt tcccgcccga aggtacacaa tcgtgatgaa acagatacca ttttccttca
     5341 aattctgtaa tacaatgatg tgtagtccat cccactaccg gggtgagaat gacaccctgg
     5401 tacgtgtaag ggccataaat attttcgctt agggcatagc agagtaaatg tgtatcaccg
     5461 gtcgaatatg aaaaatagta cttaccgttg tatttgtgca tccacgatgc ttcaaaaaat
     5521 cgtctgtcat gatcgccggc acggagcggt tctccgtttt cgtccagaat caccaaatcc
     5581 cgtggttctt cagcaaaatc gagcatattt tcgctcattt tcactatttt agcgtacagt
     5641 gccggttcat tgtcagccgg ttcgtgaccg cattctatcg ctttgttatt gcggtagcgt
     5701 tgaagctgac caccccaaag accaccaaaa cacataaagt ggctgtcgtc atcttctctg
     5761 taaacacatg gatcgatgga atagctgcct tttatcggac tatccagcgc aacgaaagga
     5821 ccttccggtt tttcgcttat ggcaatgccg atatggaata catcgttttt atctttcagc
     5881 gaaaaataaa tataatattt cccgtctttg tatgccgcat cacaatccca cagctgtctg
     5941 ccagcccagg gaatactttg cacatcgagc acgacaccat gatcagtcac ttctccatct
     6001 atgctttcca tggaaaacac atgataatcg cgcatatcga aatggtcgcc attgtcgttt
     6061 tcgggtatgc cggattctac atcgtgcgat ggataaatat agatcttccc attgaaaata
     6121 tgaactgccg gatcggcggt gtaaagattt gggactaagt atcttggttg tttcatagta
     6181 gttagtttta ttgtttcgct ttatgactgt tctttatttc tattatttct tcatcgcatt
     6241 cgcctggctt ataattgttt tcacaatcgg tttcgggcgg agttttcggt cgaaaaggag
     6301 cggataatcg gtacgtcctc tcatcggcca gttatttctc caggactgag catcgcttac
     6361 gccccaaaca gttacacggc tgattttatc gctatgtttg agaaacaatc taaaaaacgc
     6421 ggcgtaacgg tcatgtaatg cagtagccac actgtctggc aatccgttag ggtaaggatt
     6481 cattttacta tcaaactggt aattcttgcc aacatcagca ccctgataac tttcgggtgg
     6541 aggaagtacg gtcatatcca tctcggtaat catcacttta gcaccggtgg aagcaaaagc
     6601 cagaatgctt ttttcatagg catcgcaggt tggaaagttc attgtcatat gtccctgcat
     6661 cccgatacca tccactttaa tgccctgctg tttgagattg tttaccattt ttaccacagc
     6721 atccctacga ccgggatgtg ccattgaata atcgttgtaa tatagttcag catcaggatc
     6781 ggcatcatgt gcatactcaa aagccagttt gatataatct tcccccagaa tttcataaaa
     6841 tttgcttttt cgccaggaac catcgtcttc gaaagcttca ttaaccacat cccatccttt
     6901 gatacgacct ttgtaacgac ctaccagggt tgagatatgc ttaatcatcc gttgtttcaa
     6961 tacttcggga ctgacatcct ttccatcggc atcgacaaaa aaccattttg gagcttgtga
     7021 atgccatatg agcacatgcc cggtagcgta aagtttgttt ttttctgcaa aatctacaaa
     7081 tttatcagcc agagtgaagt caaatttacc ttcttcgggc tgaataacct cgctcttcat
     7141 gcaattttca gccactacgg cactgaattg ttccttcata acccggagtg aagccgtatc
     7201 cgttccgttg atctgaccag tgttcatcgc tgtgccaatg tagaatttat cagccagcgc
     7261 atctttcagt gtagcgggtt cgttcatcgt tagttttgcc gagcaggaaa atgtcgctac
     7321 aaaaacgacc aaacataaag tcaggatatt taatgatttt ttcataactt taaaatgatt
     7381 ttgtgaaata attctaataa taaatgctat ttcagattgt cctctgctct tctgctgctc
     7441 agctctgcct gaattttttc attaaatgat tttgaaatcg ggtagaataa caacgcaatc
     7501 actccgatgg taaatgttgc agctgggata agactggatg ttaattttat cccgaaaatt
     7561 gaatctgctg tttgaatagt attagggata aaaccgaatg catctatgat agcgccacaa
     7621 atagctccac caacgcctaa gccggctttc agggcaaata caatgcctgc aaaaacaaaa
     7681 ccggtagccc gacgatgatt gacccattcc gaatgatcgg ccacatcgcc catcatagcc
     7741 cacaacagcg gaatagtagg cgcgtaggca agacttttca gtatattaat tataaaaatc
     7801 agagtgatat tatcggaagg aacgacaaaa aacagcgaag tgaaaactgc cgtcagcgcc
     7861 agacaaacaa cgaaaacgtt tcgttttccg aaaatacccg acaaccagcc cgataagaat
     7921 atcacaccta ataaggtaat aatctgtccg atcatattga acaaactaaa tccaacggca
     7981 aaaacctgag aattgtccga agcaatcaat ccgaaagcat tcaggaaacc gtgcataacg
     8041 ccataagact gcccggcatc gctcaccagt ccgaaattac gcagaaaact gaacaaagca
     8101 tccttgtcaa ccacataatt gaaatagtac gacatactgc ttccccacat agacagcgtg
     8161 ataaaaagaa ataacgtcag tgcaaacatt gctttccagg gtttattgct tataatatcc
     8221 ttaaaatcct gtttaatcga tgttttttga ttaacgggtg gtgaaattcg ttctttggta
     8281 gttaaaaatg taatcagcat cagcacgact accaccactg caaataataa aatagttgat
     8341 gaccagccct tttgagcatc gccctgaccg aatttcgaga ctaacggcaa ggtaagtccc
     8401 tgtacgacga atgtggcaat ggtggctgtc acgaagcgga tggacgaaat gcttgtgcgt
     8461 tccttgatat cgctcgtcat aaccccaccc agagacgaat acggggtatt gttgaacgaa
     8521 taaatggaca tcaggaggat ataagttact ccggcgtaca ggattttagc ccgttcgccc
     8581 aaatctggcg tggagaatgc cagaataaag aaaacggcaa agggaacagc tgtccagagt
     8641 agccagggac gatattttcc ccatcgcgtg tttgtccggt cggaaagtat gcctaccatt
     8701 ggatctacaa aagcatcgaa aaggcgtgca accagcagaa tcatcccggc agcggttgct
     8761 tttattccaa atacatcggt atagaaaaat aactgaaaca tcatcatcat ctggaagatg
     8821 aggttagctg aaccatcgcc aaggctatac cctacttttt caccaaacga tactttttgt
     8881 gatttcgaat tcattgttta tgtttttatg agatttaata ttacttgctt attttgttgt
     8941 atcaatatct ttccggtgct cccaggtaac ttttcttcaa tcctcccaaa tcaatcattt
     9001 ttttttgaag cacaatgccg ggttccaaaa cccggaaacg caaggtatgt tttcctgcat
     9061 tatctatttt atgacaggta atggtttcat tgatgctgtt tgcctgccat ttttccatct
     9121 tttttatatc atacttttca ttgacattta caatctgctc agttccgtca ttaaaagaaa
     9181 ttccatagcg taaaccctta ctgtcattaa agttcagtgt tggcgattct aaaatgtgaa
     9241 caggaaatta tcctgtggtt tcaaaattga catcgtattc gagatatacg gcgtcagtaa
     9301 ttttttagaa aatattttta ggctgtttga taaatccggc tttgttccgg ttactttttc
     9361 aaagtctgac agcaggtttt gaatagcgag attaactccc gcaaaatcat taacatctgc
     9421 cagaacagta aatttactgg tgtgatctac aagcttaaaa gttccggttt catttgttct
     9481 caaaataaaa tcaggcgcat ttttggcttg aagaccaatc accaataaaa agtaataaac
     9541 aatataacac aacgacccct tcattaaaaa ctattactaa ataccgtaaa cttatatgta
     9601 aaatcaattc agttctatgt aatactaatc aaaacattaa gatgagaatc tgtgtgtctt
     9661 tttatttcag aaaaattaga taatttagaa gataatgcct tgaagtcggc attatcttct
     9721 aaataccaat attaataacc cggattttgg tatgctttct tttcatcatc gctcatatcc
     9781 aatctgtcaa tttgtgcctg aggaattgga cgcagataat gaacagcttt attgataaca
     9841 cgggtaaatg ttttgggagt atgagtacct gtatcagcaa tttcgtaagt tcctgctatt
     9901 tcttcccatt tttgagtgcg aaccaggtca aaccaacgat aaccttcacc gaagaattca
     9961 cgtgaacgtt cagccagaat gtaatttata tcaattgtag caggagttgc tgctatcatt
    10021 tcagcgctat ggtcttcaac ttttgcttta ttttcggcat tgctaaagct ccacttacca
    10081 gctcttgcac gaagtacgtt cactaatcct cttgcagtac catcattcgc gtaggtgcct
    10141 gttacggctt ttgttgttgc accttttaca gcagcttcag cggcaaccag gaaaagttca
    10201 gagaattttg caataacgaa tggacggcag gtacttccat tcggagttcc taatcctgaa
    10261 ccattgtctg tacgatatgt tccgattttc cacaaacccg ggaaatactg acggttaata
    10321 ccagatggag gaattacata atcagctctt ccggcaacta cacctgcacc gatattactt
    10381 ttaccaacgg tatagctaag tgtacttgta agtgattcat ccggtaaaaa tgaaaggatg
    10441 gcatcgcccg gagaaacagg catattgtta gcattataca acgtagtaat ggatgacatg
    10501 cttgcattct tattccagtt tccacgataa acacttgtga aagttccgtc aaaacgtgaa
    10561 tcgttggttt tatcggcaaa tgtttttgta aatactccct gaggaggagc catacgtgtc
    10621 catggacggc caccccattg tgctgcttca cgctgtacgg aactaacaag atcagttcca
    10681 tcagattttt tagcacttct tatattctcc atttgaggcg taaccatcca acaagcgaag
    10741 ttgtcaggtg ccgcaccact tccataagaa aaacttactc ctccgttata ttgttcgctg
    10801 gcatttgtat gatcagcaag taataaaatc tcgttattgc ggtcattctg cgccaggtga
    10861 actttataat aagatgattg caagcctgca taacccggac ttgtaatagc ttcagtagcc
    10921 acatcatatg cttgctggaa ataccatgct gcattatgac catcaggatc agtacgggca
    10981 gcctgaggat atgtaggaat gccgttcgga ttttctaacc accagccata agtaagatag
    11041 gctttagcaa gaaacaaacg tgcaagagtt tttgtagcag cgccaactat acgtggattt
    11101 tcaggaagat cagtaatagc ctgctttaaa tcaggaaata taccaattgt gtaaacttcg
    11161 ggaactgtat tacgtactga agttctaacg gttgatttat taaatttaag aacgccacct
    11221 cctaaatcaa ggggtactcc accaaaagtt tgtaccagct gaaaatagtc aaaagcgcgg
    11281 aagaaacgag cttcagcaat caatgacgga gctaaaccta cagcttcggc attttcgata
    11341 actccacttg cagtattaat attcgggaaa gctgcattcc aaagcacatc ggcacgtgat
    11401 gttgacggtg tcatagtacc accacttgtc atgtcggcat ctttaaagtt accatcagca
    11461 ctattgcccc aggtatattc atcagtacca gtaagtgtaa tgttataata ataaccctga
    11521 ccgtaaatat cacgcaaatg agcatacata gaagttaatc cgctctgaac tccctccttg
    11581 gtagtaaaaa agccgggttc gtatgtggca cgcggctgtt catccaagat atcgttacag
    11641 gctgacagga acaacatcat catagctccc atcaaaactt tgaaatttat ctttttcatt
    11701 attatttctt ttttaagtta ttagaatgta atactaagac ctaccaggta gttgcgtgta
    11761 gatggtgagt tagtacctac ggtaaggata cgtctgttag ctaaacctga agctgaagcc
    11821 atgttttcat caccgtaaga gtttgtttcg ggatccatac ctgttt
//
LOCUS       DAOPYB010000088        11864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6110_length_11864_cov_9.371412, whole genome shotgun sequence.
ACCESSION   DAOPYB010000088 DAOPYB010000000
VERSION     DAOPYB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6110_length_11864_cov_9.371412"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..678)
                     /locus_tag="PKH58_07895"
     CDS             complement(202..678)
                     /locus_tag="PKH58_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88986.1"
                     /translation="MKKLAIYLMAAMLTISLTLSAQDNNENRMHGNRMGQPRGMQMSA
                     QQRAENLSKQLSLTAEQTQKVQALYEKQDAKRTQQREEFGKQRKEMQQKQTNERDEFR
                     AQREKEMKEQDAELEAIIGKEKMDQLNAFREQRKQRMEQRMNNRPQGMHEGGNQQR"
     gene            complement(778..1176)
                     /locus_tag="PKH58_07900"
     CDS             complement(778..1176)
                     /locus_tag="PKH58_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNX88987.1"
                     /translation="MEKLFIEVQSNYNFDQTMEKLTETILMSGWKIPHVHDLQETMQK
                     NQFDVLPVKVLELCKPTYSVKLLERDDERIYSSLMPCRISVYNKSDGYTYVSLMNSGT
                     IAAQLGGMVELVMTDAYLESQKFIKSVIID"
     gene            complement(1272..1787)
                     /gene="trxA"
                     /locus_tag="PKH58_07905"
     CDS             complement(1272..1787)
                     /gene="trxA"
                     /locus_tag="PKH58_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX88988.1"
                     /translation="MKKLALATTLLVSLAIVSCAGTSKDKEKKGTAIVQTETKKVENN
                     KTETKKMGTIHLTKEEFLKKVVNYETNSKEWKYLGDKPCLIDFYADWCGPCKVVAPIL
                     EELATEYEGQIYIYKINTEEEQELAAAFGIRSIPSLLFVPMGEAPQMAQGALPKANLK
                     EAIDNVLLKKK"
     gene            complement(1798..2031)
                     /locus_tag="PKH58_07910"
     CDS             complement(1798..2031)
                     /locus_tag="PKH58_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNX88989.1"
                     /translation="MYSTFYVNGDGCLTCNNQVLREIGNLQGVFGAEIDRYKGEVVVT
                     HTEEVSREMIEKTLNELGFFEENNNKNHCETNT"
     gene            complement(2037..2288)
                     /locus_tag="PKH58_07915"
     CDS             complement(2037..2288)
                     /locus_tag="PKH58_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX88990.1"
                     /translation="MKQMIEYFKVWDFARWFKLGLAVAMGLAYIFGSGSIFLFGSLFF
                     SVQALFNIGCGCATGNCSTPVRKDDKQVNYDIEEYKPNK"
     gene            complement(2285..2482)
                     /locus_tag="PKH58_07920"
     CDS             complement(2285..2482)
                     /locus_tag="PKH58_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNX88991.1"
                     /translation="MVQFLKKHLIGLIGILMGAVGGYLYYYFIGCASGTCPITSNPYI
                     SVLYGALMGFLLFDLFKNKEK"
     gene            complement(2516..2809)
                     /locus_tag="PKH58_07925"
     CDS             complement(2516..2809)
                     /locus_tag="PKH58_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX88992.1"
                     /translation="MTQENSVKMEEAAYMLRAISNCTRLQVIAALSDKDELSVSQLQE
                     ALSCEQSLLSHHLTDMRAKGVLNCRREGKNCYYSLKNKQIIQILNCINSCNCN"
     gene            2987..4036
                     /gene="aroB"
                     /locus_tag="PKH58_07930"
     CDS             2987..4036
                     /gene="aroB"
                     /locus_tag="PKH58_07930"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNX88993.1"
                     /translation="MRESKTIITSDFTAELNQLLDKWQDNKIFILTDDYAEKFCLPAV
                     LECANARNSQIITIEHGDSHKTIESAVKIWSFLSEHGATRKSLMINLGGGMITDIGGF
                     TASTFKRGMSYINIPTTLLGAVDAATGGKTGINFQGLKNEIGVFAPADTVLVNVDFFR
                     TLDARNFLSGYAEMVKHALIESQEAWNKVLSFDFENVDYDNLKKLLAENIAIKQRIVA
                     EDPTEQGIRKALNLGHTFGHAFETLSHKTENPLLHGYAVAYGLVCELYLSFIKLGFPK
                     DDLLKYRHFVRENYGSFNCDCSDYESVLELMMHDKKNDEHGINFTLLSGAGEIRINQT
                     ATREEIFDCLDILFN"
     gene            4069..4425
                     /locus_tag="PKH58_07935"
     CDS             4069..4425
                     /locus_tag="PKH58_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNX88994.1"
                     /translation="MKTLIIGASKNPERYSYKAAERLLAHEHEIELLGRRADEIFGKK
                     IQTEKKKFESIDTVTLYVGPQNQPEYYDYIVSLKPRRVIFNPGTENPEFVQLLEANGI
                     LCEIACTLVLLGTNQY"
     gene            4470..5186
                     /gene="dapB"
                     /locus_tag="PKH58_07940"
     CDS             4470..5186
                     /gene="dapB"
                     /locus_tag="PKH58_07940"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNX88995.1"
                     /translation="MKIALIGYGKMGKIIERIALERGHEIVSIIDVDNRQDFKSEAFK
                     SADVAIEFTIPKVALDNYQQAFEAGVAVVSGTTGWADQQEKVKADVLKNGYTLFWSSN
                     FSLGVNIFMAVNKYLAKIMNQFPEYNVEMTEVHHTQKLDAPSGTAITLAEGILDNIDR
                     KTGWVKEIQQNDNELAIKSIREGQVPGIHTIRYESFADIIEITHDAKSREGFALGAVL
                     AAEFTCGKKGFLGMDDMLKF"
     gene            5238..>6634
                     /locus_tag="PKH58_07945"
     CDS             5238..>6634
                     /locus_tag="PKH58_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNX88996.1"
                     /translation="MNSEKQTFASRLKRPVKQWVKCTIWSLIYILFIAWVGNFWWLLL
                     WPFIFDVYITKIIPWTWWKKSKNKTVLSVMSWVDAIVFALVAVYFIHTYLFQNYQIPT
                     SSLEKSLLVGDFLAVSKASFGPRVPMTPLSFPLVQHTFPILNSKSFIEKPQWEYKRLK
                     GTDKLERNDIVVFNFPTGDTVCVKQQNPDYYTLCFLYGRDVVRSRTDIFGEIVYRPVD
                     RRENYVKRCVGIAGDWLEVKNNQIIVNGKPQEHFQGIQFNYFVQTDGTRLTDEVLDGL
                     NISKEDRILLDNSQAGGLISTLGLNPNLPVYNFPLTEESYKKMQNTRGVVKLVIEPSG
                     VLMEDHTFPLGNTYNWSRDNYGPIYIPKAGAILKLTIANLPIYERIIRVYEKNKLEVK
                     DGQIFINDQPATSYKFKMNYYWMMGDNRHKSADSRYWGFVPEDHIVGRPMFVWLSLNK
                     DKSLFEGKIRWDRFF"
     assembly_gap    6635..6734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6735..7108
                     /locus_tag="PKH58_07950"
     CDS             <6735..7108
                     /locus_tag="PKH58_07950"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNX88997.1"
                     /translation="HWRAITSAYMNSPFFEYYQDDFRIFFENKYTFLWDYNLELLTLI
                     MNLLDISPEIKLTEKYEAQYTTVTDMRDTIHPKKTPVFITKPYYQVFENKLGFQQDLS
                     IIDLLFNMGNESIVIISQPAP"
     gene            7254..8273
                     /locus_tag="PKH58_07955"
     CDS             7254..8273
                     /locus_tag="PKH58_07955"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNX88998.1"
                     /translation="MENINWSNLSFGYMKTDYNVRCTFKDGAWGEIRTCTDENITMHM
                     AATCLHYGQEAFEGLKAFKGKDGRIRIFRLDENAKRLQSSCEGIMMEKLPLEMFEEAV
                     LKVVKLNERFVPPYESGASLYIRPLLIGTSAQVGVKPAKEYEFIIFVTPVGPYFKGGF
                     ATTPYVIMREYDRAAPLGTGIYKVGGNYAASMVAGMKAHDMGYSAVFYLDAKEKKYID
                     ECGAANFFGIKNNTYITPKSTSILPSITNKSLMVLAEEMGMKVERRAVPEEELATFEE
                     AGACGTAAVISPIERIDDLLENKSYVFSKDGKPGIWSEKLYNKLRAIQYGDEPDTHGW
                     ITFVD"
     gene            8409..10529
                     /locus_tag="PKH58_07960"
     CDS             8409..10529
                     /locus_tag="PKH58_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNX88999.1"
                     /translation="MKKISVLLLSLTLFLSVKADEGMWMLPLIEKLNIQKMHGLGLTM
                     TAEEIYSDKNVSLKDAVVIFGGGCTGVMVSPEGLVFTNHHCGYDAIQKHSSVEHNYLK
                     DGFTAKELKDEIPSPGLAVTFLVKVEDVTDRVLPQIDNLNGKKRNEVQDSICKAISNE
                     ATQGTHYTAQVKPFYSGNEFYLFVYEKFTDVRFAYAPPSSIGKFGGETDNWMYPRHTG
                     DFSVFRVYCAPDGKPADYSKDNVPYSPKRFVTISNQGYQPGDYTMILGNPGSTERYLS
                     SWGINERVNNGNKARIEIRGAKQDVWKKYMLANEAINIAYAGKYARSSNYWKNSIGMN
                     SAIEKLKVVAQKQKQEKEFDEWVKADPQRVKKYGTVLKDLEDGYNTIFPYSHALSYLS
                     ESLLSGVELPRMASMVKRGIKNNDSSEKILKKAEDFYKDYYPEVDQATFAVLLDAYKK
                     SVTPDALPEIYNTIDKKFKGDYAKYAAYLFDKSVFVSPSKLSAALNKKNYNFDKDPAV
                     IFFNDVLKTMNSLNSKDYVSAVEKIDDAERLYEAGLKEIAAENNTARYPNANFTMRLT
                     YGQVGRYNPADAATYDYFTTTRGILEKENPNDPEFVVPEKLKSAILAKDFGQYVDAKT
                     GEMHVAFISNNDITGGNSGSPVFNAKGELIGLAFDGNWEALSGDIVFEPNMQRTISVD
                     IRYVLFIMEKVGGADRLIKELTIK"
     gene            10594..>11864
                     /locus_tag="PKH58_07965"
     CDS             10594..>11864
                     /locus_tag="PKH58_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNX89000.1"
                     /translation="MSDNLKEKTISALKWSTADRFGQQAIQFLVGLILANNIVPDDFG
                     LFGMLAIFNGLSFVLVESGFGQALVRKKSNDQQEFSTIFFFNVFIGILLYVILYFCAP
                     SIAAFFHQPELVNLSRFTFIVIILNAFYLVQINILGIKLDYKNITKVNFISTIISGTV
                     GATMAITGFGVWALAVQLVCYHFVRMICFFYFVRWKPLLFFSFIYIREHWHFSIGLLG
                     TGILNVIFNNIFTLIYGKFFPKEIGYYVQANKQSETVNYAFVSVLTGTTYNVFSQIHE
                     QTERLKHLLREFSHKTAVIVIPAGFFLIASAKNLIVSLIGEIWLPAVPYFQLICAANL
                     VSCLYQLNINTLNARGKSKITFRTEIIKKGLILLSVVSLFSLGSKIMIAGYVLACWIG
                     YFITMVEVKREINHYWKNQLKDILPAAGIGL"
BASE COUNT         3609 a         2379 c         2336 g         3440 t
ORIGIN      
        1 tcgaagccgt ttttccaatt aatcaattaa ccaatcatta aatcaaaatg actaacaatt
       61 cttagttgtt tttgatcttt tatttttcca actgtttacc tgcatttttt actgttcacc
      121 ttcagaaagt caattgtcat tttaggaaac gtcactttct atcaggtttt caatggcctt
      181 gttcgtttcc gcaggtaatt tttacctttg ttgattacct ccctcatgca ttccctgagg
      241 tctgttgttc attctttgct ccatgcgttg ttttctttgt tcacgaaatg cattcaactg
      301 gtccattttc tctttaccaa tgatagcttc cagttcggca tcctgttctt tcatttcttt
      361 ttctctctgc gctctgaatt catcacgttc attggtttgc ttttgttgca tttccttacg
      421 ttgtttaccg aattcttcac gttgctgagt tctttttgca tcctgttttt cgtagagtgc
      481 ctgcaccttc tgtgtttgtt ctgcagtcag gcttaattgt tttgacaaat tttcggcacg
      541 ctgttgagcc gacatttgca taccgcgcgg ctgacccatc ctgttaccat gcattctgtt
      601 ttcattatta tcctgcgcgg ataatgtcag acttatggtc agcatagctg ccatgagata
      661 aattgctaat tttttcatat acattttttt taatgtgaat aattaatcat ttactccttt
      721 gacaaaaagc aaaacgaatc gtttaaccgc ctccgaataa ttttacaaat ttaactatta
      781 gtctataatg acagatttaa taaatttttg cgattcaaga tatgcatcgg tcattacaag
      841 ttcaaccata cctccaagtt gcgcagcaat cgttcctgaa ttcataagtg aaacataggt
      901 ataaccatcc gacttattgt aaacagaaat ccggcaaggc atcaaagacg aataaatgcg
      961 ctcatcgtcc ctttcgagta attttaccga ataggtcggt ttacatagct ccagtacttt
     1021 cacaggcagc acatcaaact gatttttttg catagtttcc tgcaaatcat gcacatgagg
     1081 tatcttccaa ccagacatca ggatggtttc agtgagtttt tccatagttt ggtcaaagtt
     1141 ataattactc tgaacctcaa taaataattt ttccatttga ttgagtttta atgatagtga
     1201 ataaaaaaat ccggttgata atcattttca caaacaatca tttaaccgga tttcaaccat
     1261 attatcaaac tttatttctt ttttaatagc acattatcaa ttgcttcttt cagattggct
     1321 ttaggaagtg ctccctgagc catttgaggc gcttcgccca tcggaacaaa cagaagcgaa
     1381 ggaatgctgc ggataccgaa agcagctgcc agttcctgtt cttcttcggt gttaatctta
     1441 taaatataaa tctgaccttc gtactcagtt gcaagttctt ccaggatcgg agccactact
     1501 ttgcatggtc cgcaccagtc ggcataaaag tctatcaaac agggcttatc gcccaggtat
     1561 ttccattctt tcgaattggt ttcgtagttt actacttttt tcagaaattc ttcttttgtt
     1621 aaatgaattg ttcccatttt ttttgtttca gttttattat tttctacttt ttttgtttca
     1681 gtttgaacta ttgctgtacc ttttttctcc ttatctttac ttgttccggc acaggacacg
     1741 atggctaacg acaccagtaa agtggttgct aatgctagtt ttttcatatt tttattttta
     1801 tgtattagtt tcgcaatgat ttttattgtt gttttcttca aagaaaccga gttcgtttag
     1861 tgttttttca atcatttcgc ggctgacttc ttcggtgtgc gtcaccacca cttcaccttt
     1921 gtagcggtct atttccgcac caaaaactcc ctgtaaattt cctatttccc tcaacacctg
     1981 attattacag gtgagacatc cgtcaccgtt gacataaaat gtactgtaca tagattttac
     2041 ttgtttggtt tatattcttc aatgtcgtaa tttacctgtt tatcatcttt ccgcactggt
     2101 gttgaacagt tgccggtagc acatccgcaa ccgatattaa acaatgcctg caccgagaaa
     2161 aacaaagacc caaacagaaa aatgcttcca ctgccaaaaa tataagccaa tcccatcgca
     2221 acggctaaac caagtttgaa ccaacgggca aaatcccaga ctttgaagta ttctatcatt
     2281 tgtttcattt ttctttattc ttaaacaggt cgaacaacag gaaacccata agcgctccgt
     2341 acaatacact gatgtaggga ttggaggtaa tcgggcaagt tcctgacgca catccgataa
     2401 aataatagta taaatatcct ccgactgctc ccatcagtat tccgattagc ccgatcaggt
     2461 gtttttttag aaattgaacc atctgttata atttataata tccgtattac attcatcaat
     2521 tacaattgca cgaattaatg caattcagaa tttgaataat ctgcttgttt ttgagcgaat
     2581 aatagcagtt tttaccttca cgcctacaat tcaagactcc cttggcacgc atatcggtga
     2641 ggtgatgcga gagcagcgac tgttcgcaac tcaatgcctc ctgcagctga gatacgctca
     2701 gttcgtcttt atcggacaaa gctgcaatta cctgcaaacg tgtacaattc gatattgctc
     2761 ttagcatata tgcagcttct tccatcttta ctgaattttc ctgtgtcata ttcgaataat
     2821 tttctgcaaa tatatgaaca tatcttcata ttttcaaaat gattttagtt tttttaatag
     2881 tttctttttc aatttaccgt tatatgtata aaagcggcta attttcagac taaataaaaa
     2941 gataatttta agaaataatg tatctttgtg agctaaataa aacgcgatga gggaatcaaa
     3001 aaccattata acttccgatt ttactgcaga actcaatcaa ctcctggaca agtggcagga
     3061 taataaaatt ttcatactaa cagatgatta tgccgaaaag ttctgtttac ccgcagtact
     3121 tgaatgtgca aatgcaagaa acagccagat aatcacgata gaacacggcg actcccataa
     3181 aacaattgaa tctgctgtaa aaatatggtc ttttctgagc gagcacggag ctacccgcaa
     3241 atcgctgatg ataaatctgg gaggaggtat gattaccgat attggtggtt ttaccgcatc
     3301 aacatttaaa cgcgggatga gttatataaa tatccctact accctgcttg gtgccgttga
     3361 tgccgcaact ggcggtaaaa cagggatcaa ctttcaggga ctgaaaaatg aaatcggggt
     3421 atttgctccg gcagataccg tgctggttaa cgttgatttc ttccgtacgc tcgatgcacg
     3481 aaatttcctt tcgggttacg ctgaaatggt aaaacacgcg ttgattgaat cgcaggaagc
     3541 atggaacaag gtgctgagtt tcgattttga aaatgttgac tatgacaatt tgaaaaagct
     3601 gctggcagaa aatattgcca ttaaacaacg cattgtagca gaagatccaa ccgaacaggg
     3661 aatccgtaaa gcgcttaatt tgggacatac ctttggtcat gcattcgaaa cgttgtcgca
     3721 taaaactgaa aatccattgc tgcacggcta cgcggtggct tacggactgg tttgcgaact
     3781 gtatctgtca tttataaaac ttggatttcc gaaagatgat ttactgaaat acagacattt
     3841 cgtgcgggag aattacggca gtttcaactg cgattgcagt gattatgaat cggtacttga
     3901 attaatgatg cacgacaaaa agaatgacga gcatggaata aatttcacgt tgctctccgg
     3961 agctggtgaa atacgcatca atcagacagc caccagggaa gaaatctttg attgtctgga
     4021 tattttgttt aactgaaaac tgaaaccaaa ttaaagtaaa ccccgaagat gaaaacttta
     4081 ataatcggtg caagcaaaaa tcccgaacgg tattcataca aagctgccga acgattactt
     4141 gctcacgaac atgaaattga gttactcggc agaagagccg atgagatatt cgggaaaaag
     4201 atacaaacag aaaaaaagaa atttgaatcc attgatacgg taacgctata tgtcggtcca
     4261 caaaatcaac cggagtatta cgattatatc gtttcgctga aaccgcggcg ggtgattttc
     4321 aatcccggaa ctgaaaatcc ggaatttgta cagttgctcg aagccaacgg cattctatgc
     4381 gaaatagcct gtacactggt attgctggga acaaatcagt actgattaac ttaaactgcg
     4441 cttactattc ttataaaaaa tattgaacga tgaaaattgc attaatagga tacgggaaaa
     4501 tgggcaaaat catagaacgg attgctctcg aacgcggaca cgaaatagtt tcaatcattg
     4561 atgttgacaa ccggcaggat tttaagtcgg aagcattcaa aagcgctgat gtagccatcg
     4621 aatttaccat tccgaaagtt gcccttgaca attatcaaca ggcatttgaa gctggtgtag
     4681 cagtagtatc aggcacaact ggctgggctg atcaacagga aaaagtgaaa gccgacgtac
     4741 ttaaaaacgg ctatacactc ttttggtcat ccaactttag tctcggtgta aatatattca
     4801 tggcagtaaa caaatacctg gctaaaatca tgaaccagtt tccggaatat aacgtagaaa
     4861 tgacagaagt gcatcataca cagaaactgg atgctccatc tggcactgcc attacgctgg
     4921 ccgaaggaat tcttgacaat attgaccgga aaacaggctg ggtgaaagag atacaacaaa
     4981 atgacaatga acttgccatc aaatcaattc gtgaaggtca ggtaccgggc atacacacca
     5041 tccggtatga atcatttgcc gacatcatag aaattacaca cgatgcaaaa agtcgggaag
     5101 gttttgcgct cggagctgtg ctggctgccg aattcacctg tggtaaaaaa gggtttttag
     5161 gcatggatga catgcttaaa ttttgaaatc aataatttaa cagcattatt tagtaaaaat
     5221 catatttaca atttagtatg aactcagaaa aacaaacatt tgcatcccgg ttgaaaagac
     5281 cagtaaaaca gtgggttaaa tgcaccatct ggagccttat ttacatactc tttattgcct
     5341 gggtaggcaa tttctggtgg ttgttgctat ggccctttat tttcgatgtt tatattacca
     5401 aaattattcc atggacatgg tggaaaaaat caaagaataa aacggtgctc tctgttatga
     5461 gctgggtgga tgctattgta ttcgcactgg tagccgtgta ctttattcac acctatttgt
     5521 ttcagaacta tcagataccc acctcttcgc tcgagaaatc gctgctggta ggtgattttc
     5581 tggcagtaag caaagccagc ttcggaccgc gcgtaccgat gacgcccctt tcgtttccgc
     5641 tggtgcagca taccttcccc attctgaact caaaatcgtt tatagaaaag ccgcaatggg
     5701 aatacaaacg actgaaaggt accgacaaac tggaacgaaa cgatattgtc gtgttcaatt
     5761 tccctaccgg agacactgtt tgcgtaaaac aacaaaatcc ggattattac accctgtgtt
     5821 tcctatatgg cagggatgtt gtacgttcgc gtaccgatat tttcggcgaa attgtttacc
     5881 gccctgttga ccgccgcgaa aattatgtaa agaggtgtgt aggcattgcc ggagactggc
     5941 tggaagtaaa aaacaatcag ataatagtaa acggtaaacc acaggaacat tttcagggaa
     6001 ttcagttcaa ttacttcgta caaaccgacg ggacacgcct taccgacgaa gtgcttgacg
     6061 gattgaacat cagcaaggaa gatcgtattt tattggataa ttcgcaggca ggtggtttaa
     6121 taagcacact cggtttaaat ccaaatttgc ccgtttacaa ttttccgtta actgaagagt
     6181 cttacaaaaa aatgcaaaat acacggggag ttgtaaaact ggttattgaa ccttccggag
     6241 ttctgatgga agatcatact tttccgctcg gcaataccta caactggtct cgcgacaact
     6301 atggtccgat ttacattcca aaagcgggtg caatattgaa actcaccata gccaatcttc
     6361 cgatttacga acgcatcatt cgcgtgtacg aaaaaaacaa actggaagtg aaggatggtc
     6421 agatatttat caatgaccaa cctgccacca gttacaagtt taagatgaat tactactgga
     6481 tgatgggtga caaccgtcac aaatcggccg attctcgcta ctggggattt gttccggagg
     6541 atcatatcgt aggacgaccg atgtttgtgt ggctttcgct caacaaggat aaatcactct
     6601 ttgaaggaaa aattcgctgg gacagatttt ttaannnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnntacact ggcgtgcaat aacgtcggct tatatgaaca gtccgttttt
     6781 tgagtattac caggatgatt ttcgtatatt ttttgaaaat aaatatactt ttctttggga
     6841 ttacaatctt gaattgttaa ctttgataat gaatttgctg gacatttcac ccgaaattaa
     6901 gttgaccgaa aagtatgaag cacaatacac aacggtaacc gatatgaggg acacgataca
     6961 tccgaaaaaa acgccggttt ttattacaaa accctactat caggtcttcg aaaacaaact
     7021 cggatttcaa caggatttaa gtattattga tttgttgttc aatatgggga atgaaagtat
     7081 agttataatc tcacaaccag ccccctaacc ccctgaaggg ggaatatgtg ataaaaaatt
     7141 agtggtttta aaaatttcga tatataaaat aaatagttct ttacaaaata aataaaaaca
     7201 taaacgctct caaattctcc atcagggggt tagggggcag ataattaaat tttatggaaa
     7261 acataaactg gagtaatttg agtttcggtt atatgaaaac cgactacaac gtacgctgta
     7321 ctttcaaaga cggggcctgg ggtgaaatca ggacctgcac agatgaaaac attactatgc
     7381 acatggcagc cacctgcctg cattatggtc aggaagcatt tgaagggctg aaagcattca
     7441 agggtaaaga tggcagaata cggatattcc gtctggatga aaatgccaaa cgcctgcaat
     7501 cttcgtgtga aggtattatg atggagaaac tgccgctcga gatgtttgaa gaagctgttt
     7561 taaaagtggt taaactgaat gaacgatttg ttccgcctta cgaatcggga gcatctcttt
     7621 atattcgtcc tttgctcatc ggcaccagcg ctcaggtagg tgtgaaacct gcaaaagaat
     7681 acgaatttat catttttgta actccggtag gcccttattt caagggagga tttgctacca
     7741 caccctacgt aattatgcgc gaatatgacc gtgccgcacc acttggcaca ggaatctaca
     7801 aagtaggggg taattatgca gcaagcatgg tagccggcat gaaagcacac gatatgggct
     7861 attcggcggt attctatctc gatgccaaag aaaagaaata tattgatgaa tgcggtgctg
     7921 ccaatttctt cggtataaaa aacaatacct acatcactcc aaaatcaact tcaatcctac
     7981 cctccattac caacaaaagt ctgatggttc tggcagaaga aatgggtatg aaagtggaac
     8041 gccgcgcagt tcccgaagag gaactggcta ccttcgaaga agccggagca tgcggcacag
     8101 cagctgttat cagtccgatt gaacgtattg acgacctttt ggaaaacaag tcttacgtat
     8161 tttccaaaga tggaaaaccc ggcatatgga gcgaaaaact atacaataaa cttcgcgcca
     8221 tccagtacgg tgatgaacct gatacacatg gctggattac ctttgtagac taaatttcaa
     8281 gcatttacag agttatattt acagataaac agataattct gctaccggta aacaaaacca
     8341 acttaataaa aacacacaag atttgacggc aataaaaatt tgcccggaat aacacaaaaa
     8401 attcaagaat gaagaaaatt tcagtattac tgctttcact cactttattt ctgagtgtaa
     8461 aagccgacga aggtatgtgg atgttacctt taatagaaaa acttaacatt cagaaaatgc
     8521 atggactggg actgaccatg actgccgagg aaatttacag cgataaaaat gtaagtctga
     8581 aagatgctgt agtaatcttt ggaggcggtt gcaccggtgt gatggtatcg cccgaaggtc
     8641 tggttttcac caatcaccac tgcggttacg atgccataca gaaacacagc tcggtagaac
     8701 acaactacct gaaagatggt tttaccgcca aagaactgaa agatgaaatt ccatcaccgg
     8761 gtcttgccgt aacttttctg gttaaagtag aagacgtaac cgaccgcgtt ttaccccaga
     8821 tagacaatct gaatggtaaa aaacgtaacg aagtacagga ctcgatctgc aaagccatca
     8881 gcaatgaagc cacacaggga acacattaca cagcacaggt aaaacctttc tattcaggta
     8941 acgaattcta tttattcgtt tacgagaaat ttactgatgt tcgttttgct tatgcacctc
     9001 catcgtctat cggaaaattt ggcggtgaaa cagacaactg gatgtatccg cgtcacaccg
     9061 gcgacttttc ggtattccgg gtttattgtg cacccgacgg aaaaccggct gattattcaa
     9121 aagacaatgt gccttacagt ccgaaacgat ttgttactat ctccaaccag ggttatcaac
     9181 ccggcgacta taccatgatt ttgggtaatc cgggatcaac agaacgctac ctgagttcgt
     9241 ggggcatcaa cgaacgtgta aataatggca acaaagcccg tatcgaaatc aggggagcaa
     9301 aacaggatgt gtggaaaaaa tacatgcttg ccaacgaagc aataaacatt gcttacgcag
     9361 gaaaatatgc acgcagttcc aattactgga aaaacagcat tggtatgaac agcgccatag
     9421 aaaaactgaa agttgtagca cagaaacaaa aacaggaaaa agaatttgac gaatgggtaa
     9481 aagcagaccc gcaacgggtg aaaaagtacg gtacagtact gaaagacctc gaagacggct
     9541 ataataccat tttcccctac agtcatgctt taagttacct gagtgaatcg ttgctttcgg
     9601 gcgttgaact tccacgtatg gccagcatgg taaaacgggg aataaaaaac aacgactctt
     9661 cagagaaaat attgaaaaaa gcagaagatt tctacaaaga ttactatcct gaagtcgatc
     9721 aggcaacatt cgccgtattg ctcgacgctt ataaaaaatc ggttaccccc gatgctttac
     9781 cggaaatata taacaccata gataaaaagt tcaaaggtga ttatgccaaa tatgcagcct
     9841 atctttttga caagtcggta ttcgtatctc cttcaaaact aagcgctgct ctcaacaaaa
     9901 agaactacaa tttcgacaaa gatcccgctg ttatcttttt caatgacgtt ttgaagacaa
     9961 tgaattctct gaacagcaaa gactatgtaa gtgctgttga gaagattgac gatgctgaac
    10021 gtttatacga agccggtctg aaagaaattg ccgctgaaaa taacacagca cgttatccga
    10081 atgctaactt caccatgcga ttgacatacg gacaggttgg ccgttacaat ccggctgacg
    10141 cagcaacata cgactatttt acaacaacca gaggaattct ggaaaaagag aatccaaatg
    10201 atccggaatt tgttgtaccc gagaaactga aaagtgcaat tctggcaaaa gacttcggtc
    10261 agtatgtgga tgctaaaacc ggtgaaatgc atgtagcgtt tatcagcaac aatgacatca
    10321 ccggaggcaa ctcgggaagt cctgtgttca atgctaaagg agaactgatc ggactggcat
    10381 ttgacggcaa ctgggaggcc ctgagcggcg atattgtatt cgagcccaac atgcaacgca
    10441 ccatcagtgt tgatatccga tatgtacttt tcataatgga aaaagtaggt ggtgccgacc
    10501 ggttgatcaa agaactgacg ataaaataat ataaaaaaga gaatcctata aaaaagcagt
    10561 tggcagctac atgtcaactg cttcattata tttatgagcg acaacctgaa agaaaaaacc
    10621 atcagtgcgc tgaaatggtc aacggctgat cggtttggtc agcaagccat tcagtttctt
    10681 gtaggtctga tactggccaa taatattgtg cctgacgatt tcggactttt cggcatgctt
    10741 gcaattttca acggattgtc gtttgttttg gttgaaagtg gtttcggtca ggctttagtc
    10801 cgcaaaaaat caaatgacca gcaggaattc agcaccattt ttttcttcaa tgtttttatc
    10861 ggcatcttac tctatgtcat tttgtatttc tgcgctccat ccattgccgc tttctttcat
    10921 cagccagaac ttgtcaatct ctcaagattt acatttattg taatcattct taatgcattt
    10981 tatcttgtac aaattaatat tctggggata aaactcgatt acaagaacat taccaaagtc
    11041 aatttcatat ccaccattat cagtggcact gttggtgcaa caatggctat cacgggtttt
    11101 ggtgtatggg cattagccgt gcagcttgtt tgttatcatt tcgtccggat gatttgtttt
    11161 ttctattttg tcaggtggaa accgttgttg ttcttttcat ttatttacat tcgtgagcac
    11221 tggcatttca gcatcggatt gctgggtacc gggatattga atgtcatttt caacaatatt
    11281 ttcactctga tatacgggaa gttttttccg aaagaaattg gatattacgt tcaggccaac
    11341 aagcaaagtg aaaccgtaaa ttatgctttt gtgtcggtac tgacaggaac aacctacaat
    11401 gtattttctc aaattcatga gcaaaccgaa agactaaaac atcttcttag agaattttcg
    11461 cataagacgg cagtcattgt gatacctgcc ggattttttc tcatcgccag cgccaaaaat
    11521 ctcatcgtat cgctgattgg tgagatatgg ttgccggctg taccatattt tcaactcatt
    11581 tgtgccgcca atcttgtttc atgcctttat cagcttaaca ttaacactct gaacgcacgt
    11641 ggtaaatcaa aaatcacctt ccgaacagaa atcatcaaaa aaggattgat tttactttcg
    11701 gtggtgtcat tgttcagcct gggaagtaaa ataatgatag ccggttatgt cctggcctgc
    11761 tggattggct atttcattac catggtggaa gtaaaacgtg aaattaatca ttactggaaa
    11821 aatcagttga aagatattct gcctgcagca ggcatcgggc tatt
//
LOCUS       DAOPYB010000089        11820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6146_length_11820_cov_7.436209, whole genome shotgun sequence.
ACCESSION   DAOPYB010000089 DAOPYB010000000
VERSION     DAOPYB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11820
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6146_length_11820_cov_7.436209"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="PKH58_07970"
     CDS             complement(<1..2026)
                     /locus_tag="PKH58_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX89001.1"
                     /translation="MKNKILSYSDKLVYLKKTSLVRYVLLMGLVFFTASFSASAENSL
                     QSQQPKKTVTGIVSDSSGEPIIGVTVREQGTKNGVITNVDGRYTINVGNDAVLSFSSV
                     GYKNVIQEVGVNRTLNITLPDDIATLDELVVVGYGTVKKSDVTGALIQMSEKTIKERP
                     VQNAIQAMQGKAAGVDIQTNGRPGEISAVVIRGTRSINASNSPLYVVDGVILMGDMND
                     INPNDIASMEILKDASSTAIYGSRGANGVVLITTKSGKKNVMSVDYNTTVSFDRINSL
                     TDWASAGEMLDRMRTANINGSNYKSGSVSLNYPDPTADITSFGNGDAYTIDAIRSAYE
                     WNDPGTFSSVKMRSSTADEKLMGYPDMVPLYDSSKIPTTDWIDMLTRTGLTQSHQIGI
                     SAGTDKSKLYTSIGYYGNKGAQMNQGYNRYTFRLNGDINPLSWLNIGASVNTSYSEQQ
                     YGRINRSGSATGANDSYGMALGQYLMAQPYDKDGNIIEYPGNNAGAPVWNPYIDLQNS
                     EDLTRKVNIQANTYLGIKFAPWISYRMNFGTGYRVNRSGTYQGSESTLRRKTVGAGSA
                     ATFSNSDNFQYLIENIIYLNKKMGIHDLSATLMQSAQYSRTESSYIEASKILYDTSRW
                     YNLAANLNGKPDDYGSGFTESSMVSYMGRVNYSLLERYLLTASLRYDGASV"
     gene            2763..4247
                     /gene="rhaB"
                     /locus_tag="PKH58_07975"
     CDS             2763..4247
                     /gene="rhaB"
                     /locus_tag="PKH58_07975"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HNX89002.1"
                     /translation="MIENTETSFLAIDLGASSGRAILGTIQDNRVELKEINRFVNPII
                     DVNGRSYWDLFHLYSEILKSLKLIKSEGVQIVSLGVDTWGVDFVCFGKDGEPLRMPYC
                     YRDTHTFGAPERFFEKMSKKDVYMKTGIQIMNFNTLFQLSTQHEENNSIYPFVDKILF
                     MPDALSYLLTGQMVTEYTIASTSQMLNPYKRTFDTSLLEMINLSADKFAPVVSSGTVI
                     GKLSASVMHQTGLDDISVVAVAGHDTASAVLATPAENENFAYLSSGTWSLMGIESTEP
                     VINEETYNLNFTNESGSDGSIRLLKNICGMWLIEQCKKEWETKGTVTYQEIVQAAEAA
                     TPFKCFINPDSPCFASPQSMINAIQTYCNQTGQFVPQSMGEIARCIYESLAFRYKQVL
                     ANLQNLATFPIHTLHIIGGGSQNTMLNSFTANAIGKSVIAGPSEATAIGNILLQAQAA
                     GLVKDKKEIRQIIRNSVELKTIEPQNTSLWEENYTNYLKTFKEL"
     gene            4268..5527
                     /locus_tag="PKH58_07980"
     CDS             4268..5527
                     /locus_tag="PKH58_07980"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HNX89003.1"
                     /translation="MKSEQIKQAYEDAKVQYAALGIDVDKALKQIDELSISIQCWQSD
                     DVTGFENPDGQLTGGIQATGNYPGKARTIDELKQDIEKVIKLIPGKHRLSLHAIYGDF
                     GGEFVDRDQIEPKHFQSWIDWAKEVGIKIDFNSTFFSHPKSGNYSLSSFDPEIRNFWK
                     NHLRACRKIAAEIGRQQGSPCVHNIWVHDGEKDKTVSRYAHRKLLQESLDEVLAEKYD
                     TNYLKDSIECKLFGIGSECYVVGSHEFYMGYAVKNNMLLTLDAGHFHPTEGIADKISS
                     MLLYLPEIALHVSRPERWDSDHVVILNDPLIELSQEIVRSGQMKRVNVGLDYFDASIN
                     RIGAYVVGIRSTQKAMLQAMLEPTEKLREYEIQDKNFQRMAYLEELKALPWNAVYNYY
                     CLQQGIAVGDAYIKEIEDYENAVTSKR"
     gene            5534..6565
                     /gene="rhaT"
                     /locus_tag="PKH58_07985"
     CDS             5534..6565
                     /gene="rhaT"
                     /locus_tag="PKH58_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HNX89004.1"
                     /translation="MNTIIGLLIIAVGSMGQSSSYVPIKKVKTWAWESFWLVQGIFAW
                     LIFPYLGALLAVPDGHSLLEILSSGYGAAWKAMFFGALWGVGGLTFGLSMRYLGVALG
                     QSIALGTCSAFGTLIPALMTGTDLLHGKGLVLLIGVSVAIAGIAVIGYAGSLRAKNMT
                     EEQKKAAIKDFALGKGLLVAILAGVMSACFSLGLEAAEPLKAAALSMGTKALYAGLPA
                     ILMITLGGFVTNAFYCIYQNFKNKTQKDYFKVPGNVLLNNLLFCALAGILWYSQFFGL
                     ALGKSFLTEGSTMMAFSWSILMSLNVTFSNVWGIILKEWKGAGKKAVGYLVIGMLILI
                     FSLIIPSLF"
     gene            6625..7506
                     /locus_tag="PKH58_07990"
     CDS             6625..7506
                     /locus_tag="PKH58_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX89005.1"
                     /translation="MRFNDFAIDFKYLIVNERDKKFGLTVNTVGFQPIGPNTLYPSTE
                     HPKKYFFDPSKGRVLPEFQFVYISKGKGVFTSETTKKQTVRKGQIIILFPGQWHTYCP
                     DPDTGWNEYYIGFEGSIINTVIQNSFISMENQILDVGFNEDLVHLFSTAIKVAKEDKK
                     SSQQYLSGIVFHILGTILSLSENKKIESNEYGQIIERAKMIMLENVKNIIDIQELASN
                     LGTSYSWFRKVFKEYTGYSPAHYFQELKLRKAKELLAETSIPIKEIAYELNFSSYGYF
                     LSFFKKRVGCTPFEYRS"
     gene            complement(7612..8346)
                     /locus_tag="PKH58_07995"
     CDS             complement(7612..8346)
                     /locus_tag="PKH58_07995"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNX89006.1"
                     /translation="MKILFSTSTSPSFNLATEEYLFSDKSEEYLFLYVNQPSVVIGCN
                     QAVSNEVNMGFCFQNNIQIVRRLSGGGTVYHDEGNLNFCFIHNKRSEQSSLSAGFLKP
                     VINVLHSMNIPVEAGKRKDLWLDNFKVSGTASHISRNRELHHGTLLYSSDIHALQKTL
                     TPNKSEVFKKAIRSVSSPVKNIFTYLTENNRIPASEADFFHEFALKLAEYFNITELSE
                     LSDENIREIEIIRQNRYSRIEWNFKI"
     gene            complement(8519..9817)
                     /locus_tag="PKH58_08000"
     CDS             complement(8519..9817)
                     /locus_tag="PKH58_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNX89007.1"
                     /translation="MKQLDVKMMHPVEQINVIIERIYRSGMTTTSGGNISIKDENGDI
                     WITPSAVDKGNLTTKDIMCVKKDGTVVGPHKPSSEFPFHKAIFEARPELKAVIHAHPP
                     ALVAFSIARTVPDTKIVPQAHNICGDIGFAEYGCPGSEDLGEKIARQFKNTSYKAVIM
                     ENHGVVLGGTDMMDAYQRFETLEFCCRTIINAKKLGGVNYLTDEEVSKYVHHIPSNVP
                     HFMDISYPSDERALRSDMVKIIRRACNQGLMISTYGTVSVRWKDGDFLITPRDVARWD
                     IMPEDIVQIKNGMAEAGKTPSRSVALHQRIYQLNPHINSIICTQPVNLMAHAVSGKKF
                     DVRTIPESWIFLQDVPSIPFGNIYKEIDEVAQMFADNRVILVENDSVFVTGDKLLNTF
                     DYLEVAEFSANSLVMATSIGELKPMGDKEIDELRVAFNVK"
     gene            complement(9875..11188)
                     /locus_tag="PKH58_08005"
     CDS             complement(9875..11188)
                     /locus_tag="PKH58_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNX89008.1"
                     /translation="MKTKAIRLYGKKDLRMEEFELPQIKENEILAKVVSDSLCMSSYK
                     ASTQGTDHKRIPKDVAENPIIIGHEFAGELVEVGAKWSHKFKAGDKFSIQPALNFEKG
                     PVGVLSAPGYSYQYIGGDATYVIIPAEVMEKDCLLPYHGEGFYPASLAEPLSCVIGAM
                     HANYHITPGSYIHKMEIVDGGKMAILAGVGPMGLAAINYILHREDRRPSLVVVTDVDQ
                     SRLDRAATLYTVQSAALRGIQLIYINTGNMKNPVEELKAITNGVGYNDVFVFAPVGPV
                     VEQADAILAFDGCLNFFAGPSDPNFSAKFNFYNVHYGYTHVVGTSGGNNDDMVEALRI
                     MSKGLDPAGLITHIGGLDAVIEATNHLPEIPGGKKLIYTHITMPLTAIEDFAGLGETD
                     DLFRNLAEICNRYNGLWNVEAEKYLLENCPPNPLKGGLRERVSTI"
     gene            complement(11294..>11820)
                     /locus_tag="PKH58_08010"
     CDS             complement(11294..>11820)
                     /locus_tag="PKH58_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX89009.1"
                     /translation="DMTPENFDLVTKINYNAYFYCTKVVSKVMKLQTKYASENYFADI
                     IQINSKSGLRGSKANFAYAGGKFGGIGLTQSFALELASFRIKVNSICPGNFYDGPLWS
                     DPVNGLFVQYLNAGKVPGAKTIDDVKAFYLSQVPMRKGCTPEDVTKGVLYLMEQCGET
                     GQALPISGGQVMLN"
BASE COUNT         3463 a         2510 c         2399 g         3448 t
ORIGIN      
        1 gtaccgaagc accatcgtac cgaagagatg ctgtcaacag gtatctttcc agtaaactgt
       61 agttgaccct gcccatatag gacaccatcg aactttcggt gaagccggaa ccataatcgt
      121 cgggtttacc attaaggtta gcagccagat tataccacct ggaagtatcg tacaatattt
      181 ttgatgcttc gatatatgaa ctttcggttc gggaatactg tgcagattgc ataagtgtag
      241 cactcaggtc gtgtatgccc atttttttat tcaggtaaat gatattttcg ataagatact
      301 gaaaattatc gctattggag aaggttgctg ccgatccggc tccgacagtt ttcctgcgga
      361 gagtggattc ggaaccctga taggtacctg aacgatttac gcgataacct gtaccgaaat
      421 tcattctgta cgaaatccag ggagcaaatt ttattccaag gtaagtattg gcctgaatat
      481 taacttttcg ggtcaggtct tcgctgtttt gaagatcaat ataggggttc catacaggag
      541 caccggcatt attacccgga tattctatga tattgccatc cttatcgtaa ggctgagcca
      601 tcagatactg tcccagagcc ataccatagg agtcgtttgc accggtagca cttcctgaac
      661 ggtttattct accatattgc tgttcgctgt acgatgtatt taccgaagca ccgatattca
      721 gccagcttaa cggattgata tccccattta accggaaagt gtagcggttg tagccctgat
      781 tcatctgtgc acctttattg ccgtaataac cgatggatgt atataatttt gatttgtcgg
      841 taccggccga aattcctatc tgatgacttt gagtcagacc ggtacgggtt aacatgtcaa
      901 tccagtcggt tgtagggatt ttggaagaat catacagagg taccatatcg ggatatccca
      961 tcaatttttc gtcggctgtg gatgatctca ttttcacaga agaaaaagtg ccgggatcat
     1021 tccattcata cgcgcttctt atggcgtcga tggtgtaggc atcgccattt ccaaaagaag
     1081 tgatatctgc agtcggatca ggatagttca gggaaactga acccgattta tagttggaac
     1141 cgttgatgtt tgccgtcctc attctgtcaa gcatttcacc ggctgaagcc cagtcagtca
     1201 gggaattaat cctgtcaaaa gaaactgtag tgttgtagtc aaccgacatg acattctttt
     1261 taccgctttt ggtagtgata agcacaaccc cgttggcacc tctcgaaccg taaatggctg
     1321 ttgatgaggc atcttttaaa atttccatag aagcaatatc attgggattg atatcgttca
     1381 tatcacccat aagtataaca ccatcaacca catacaaagg agagttggag gcattgattg
     1441 atcttgtacc acgaataacc acagccgaaa tttcaccggg acgtccatta gtctggatat
     1501 ctacaccagc agctttaccc tgcatagcct ggatggcatt ctgcacaggt ctttccttaa
     1561 tggttttttc agacatctgg attagagctc ccgtaacgtc gcttttcttc acggttccgt
     1621 aacctactac caccagctcg tcgagagtgg caatatcatc aggcaaggtg atgttcagtg
     1681 ttctgttgac acccacttcc tgtatcacgt ttttatagcc aacactactg aatgacaaca
     1741 cggcatcatt ccctacatta atggtataac ggccatcgac attggtaata actccgtttt
     1801 tagtaccctg ttctcttacg gttaccccta taataggttc gcccgaacta tcggatacga
     1861 taccggtaac agttttttta ggttgttgcg attgaagact gttttccgcg gacgcagaaa
     1921 aacttgcagt aaaaaagaca aggcccatta acaatacata acgaaccagg ctcgtttttt
     1981 tcagataaac aagtttatcc gaataactaa gaattttgtt tttcatttgt taaaaattta
     2041 atttttaagg taatcgatta tatggtatta tggaataatt ggcattcgtg gagtaaattg
     2101 aaatagcata aataatgcac tttcattcac ccatctataa tggttccgga taacagagag
     2161 tagatttaag agaaataagg taaaatttca atcgcaaaaa taatatctgt tttattccta
     2221 aaagtatgaa aaacgatatt aaaagttaga tttttgatat tttttcatgt ggcaattacc
     2281 caaaggtgtt gttccataat tactgattat ctgtttatta gaattagcta ttaataaata
     2341 acattctggt actaaaaaat aaaagatagc ataatgtaag gatatatgct tagttttcga
     2401 tgcatgcatt tttatctgat ttggctctca tgttaaataa agcagatcac cggaaaaaaa
     2461 tcacttatgc ttagaaacaa tatttattta ttaaaactga cagcattcat aatacatata
     2521 tatggcagta gaaatcaaca tctgaaatat ttaagctccg caatgacgcg acaaattatc
     2581 aatgaataaa tactgcgtct ttggggctta tgtataatta catgttacaa gtccggattt
     2641 ttcatatcca tacctgtaaa aaataatatc aaataatgta tttattaaat cattttttca
     2701 tcattccgat tttcaataat ctctattttt gcgatgttat atttattaat tttagattta
     2761 tcatgattga aaacacagaa acctcgttct tagccatcga tttaggagca tcaagtggaa
     2821 gagcaatact gggaaccatc caggacaacc gggtggaatt aaaagaaatc aatcgttttg
     2881 tcaatccaat tattgacgtc aacggacgct cgtactggga cctgtttcat ttgtacagcg
     2941 aaatactgaa atcactgaag ctaataaaat cggagggtgt acaaattgtc tctttgggtg
     3001 ttgacacctg gggcgttgac tttgtttgtt tcggaaagga cggtgaaccc ctgagaatgc
     3061 cttactgcta cagggacacg cacactttcg gtgctccgga aagatttttt gagaaaatga
     3121 gcaagaaaga cgtctatatg aaaaccggta tccaaataat gaatttcaat accctttttc
     3181 agctttccac gcaacatgag gaaaataatt ccatttatcc gttcgtcgat aagatactct
     3241 tcatgcccga cgcgttgtcg tatttactta ccggacaaat ggttacagaa tacaccatcg
     3301 cttccacctc gcaaatgctt aatccttata aacgaacttt cgatacatca ttgcttgaaa
     3361 tgatcaacct gagtgccgat aaatttgctc cggttgtttc ttcgggaact gtcatcggaa
     3421 aactgtcggc atctgttatg catcaaacag gactcgatga catttcggtt gttgctgttg
     3481 ccggacatga caccgcttcg gctgtactgg ctacacctgc cgaaaacgaa aattttgcct
     3541 atctgagttc aggaacctgg tcactcatgg gtatcgaatc gacagaacct gtcattaacg
     3601 aagaaaccta taacctcaat tttacaaatg aaagcggctc agatggttct atccggctac
     3661 tgaaaaatat ctgcgggatg tggctgatcg aacaatgcaa aaaagaatgg gaaacaaaag
     3721 gaacggtaac ctatcaggaa atcgtacagg ctgctgaagc cgcaacacct ttcaaatgct
     3781 tcattaaccc ggactctcct tgttttgcaa gccctcaatc catgataaat gccattcaaa
     3841 catactgcaa tcaaacagga caatttgttc cgcaatcgat gggtgaaata gcacggtgta
     3901 tatacgaaag cctggcgttc cgttataaac aggtactggc caatcttcaa aacctggcca
     3961 ccttccccat ccacacactg catataatcg gtggaggatc acagaataca atgctcaatt
     4021 catttactgc aaatgcaatc ggaaaatcag taattgcagg cccatcggaa gcaacggcta
     4081 tcggaaacat actgctgcag gcacaagccg ccggattggt taaagataaa aaagaaatcc
     4141 gtcagattat ccgaaattca gtggaactga aaactattga acctcagaac acttccttat
     4201 gggaagaaaa ctatactaac tacctgaaaa cattcaaaga attataaata ataaaaagac
     4261 attttttatg aaatcagaac agattaaaca agcatatgaa gatgcaaaag ttcaatatgc
     4321 agcgttgggt attgatgtgg acaaagccct caaacaaata gacgaattat ccatttcgat
     4381 acaatgctgg caatcggacg atgtaaccgg tttcgaaaac cccgacggac aacttacggg
     4441 aggaattcag gcaaccggca attatccggg gaaagcacgc accattgatg aactgaaaca
     4501 agacattgaa aaagtaatca aactcattcc gggcaaacac cggttgagct tacatgcaat
     4561 atatggtgat tttggcggtg aatttgttga cagagatcag attgaaccca aacattttca
     4621 gtcgtggatc gactgggcaa aagaagtggg aataaaaatt gatttcaact ccacattttt
     4681 ctcgcatccg aagtctggaa attattccct ttcgagcttt gaccccgaaa tcagaaattt
     4741 ctggaaaaac caccttcgcg cctgccgtaa aatagctgca gaaattggtc gtcaacaagg
     4801 atctccctgc gttcacaaca tctgggtgca tgatggagaa aaagacaaaa ccgtttcacg
     4861 ctacgcgcat cggaagttac tccaggaatc gcttgacgaa gtacttgccg aaaaatacga
     4921 caccaactat ctgaaagaca gcatcgagtg taaacttttc ggaataggca gtgaatgcta
     4981 tgtagtaggt tcacacgaat tttatatggg ttatgccgta aaaaacaaca tgcttctcac
     5041 tctcgatgca ggtcacttcc accccacaga aggtatcgcc gataaaatat catcgatgct
     5101 gctttacctt cctgaaattg ccttgcacgt tagccgtccg gaacgctggg acagcgatca
     5161 cgtagtgatt ctgaacgatc cgttgattga actctcgcag gaaattgttc gctcaggcca
     5221 gatgaaacgt gtcaatgtag gtctggatta tttcgatgca tccatcaacc gtatcggtgc
     5281 atacgtagtg ggtatccgtt ctactcagaa agcaatgctt caagccatgc tagaaccaac
     5341 cgaaaagctg agggaatacg aaatacaaga caaaaacttc cagcgaatgg cttatctcga
     5401 agaattgaaa gcgttgccct ggaatgctgt atataattac tactgcctgc aacagggaat
     5461 agctgtgggc gatgcatata tcaaagagat tgaagactac gaaaacgctg ttacatctaa
     5521 aagatagcag gagatgaaca caataatagg tctgctaatc attgcagtag gaagtatggg
     5581 tcagtcaagc tcatacgttc ctatcaaaaa agttaaaacc tgggcttggg aaagtttctg
     5641 gctggttcag ggcatttttg cctggctgat atttccctat ctcggagcat tgctcgctgt
     5701 tcctgacgga cattctttac tcgaaattct ttcgtcgggc tacggcgcag cctggaaagc
     5761 catgtttttt ggtgcactct ggggagttgg tggccttacc tttggtctga gcatgcgcta
     5821 tctgggcgtt gctctcgggc agagtatcgc gctcggcacc tgttcggctt tcggaacact
     5881 cattccggca ctgatgacgg gtaccgattt gctgcacggc aaaggactcg tacttttaat
     5941 cggtgtaagt gtagccattg caggtatagc agtaatcgga tatgccggaa gtcttcgtgc
     6001 taaaaacatg accgaagaac agaaaaaagc agccatcaag gatttcgcgc taggcaaagg
     6061 attacttgtc gctattctgg ccggtgtgat gagtgcgtgt ttcagccttg gtctcgaagc
     6121 agccgaaccg cttaaagcag ctgccctgag catgggaacc aaagcattgt atgctggact
     6181 tcctgccata ctgatgataa cactgggagg ttttgtaacc aacgctttct attgtattta
     6241 ccagaatttt aagaataaaa cacaaaaaga ctatttcaaa gtgccgggta atgttcttct
     6301 gaacaatctg ctgttctgtg ccctggcagg aattctgtgg tattcgcaat ttttcgggct
     6361 ggcactcgga aaaagtttcc tgacagaagg cagtaccatg atggcatttt cgtggagtat
     6421 tctcatgtcg ctgaatgtaa ccttcagcaa tgtatgggga ataattctga aagaatggaa
     6481 aggagccggt aaaaaagctg taggatatct cgttatagga atgttaatat tgatcttttc
     6541 tttgataata cctagtttgt tctgagattt tttttaattt tgtcgttaat acctcaaaat
     6601 ctgaaacaac acaattgatt tgccatgaga tttaacgact ttgccattga cttcaaatat
     6661 ttgatagtca atgaaaggga taagaaattt gggttgactg tcaacactgt tggctttcaa
     6721 cccattggtc caaatacact atatccatcg accgaacatc cgaagaaata tttctttgat
     6781 ccaagcaagg gacgggtatt gcccgaattt cagtttgtat atatcagtaa agggaaaggt
     6841 gtttttacat ccgaaacaac aaaaaaacaa acagtacgaa aaggtcaaat cattatttta
     6901 ttccccgggc agtggcatac atactgtccc gacccggata ccggctggaa tgaatattat
     6961 attggatttg aaggttcaat tataaatact gttattcaga acagttttat ctcgatggaa
     7021 aaccagattc tggacgtagg atttaacgaa gatctggtgc atctgttttc cacagccatt
     7081 aaagtagcta aagaagataa aaaatcatca cagcaatacc tttcgggcat cgtatttcat
     7141 attctgggca caatattgtc attatccgaa aataaaaaga ttgaatccaa tgaatatggt
     7201 cagattattg aaagagcaaa aatgattatg cttgaaaatg tgaaaaatat tattgacatt
     7261 caggaacttg cgagcaatct tggcaccagc tattcgtggt tcagaaaagt ttttaaagaa
     7321 tatactggtt actcacccgc ccactatttc caggaactca aacttcggaa agccaaggaa
     7381 ttgctggcag aaacatccat accgatcaag gaaattgctt atgaactgaa tttcagttcg
     7441 tatgggtatt ttctgtcatt cttcaaaaaa cgggtgggat gcaccccttt cgaatacagg
     7501 agttaaaatc atcacgcagt aaagtattca ttataaaaag gcagtgaaca ggttacaacc
     7561 agcttcactg ccttttaaat acaatatcat tttaccttca acacttttac tctatatttt
     7621 gaaattccat tcgattcgcg aatacctatt ctgccggatt atctctattt cacgtatgtt
     7681 ctcgtctgac aattctgaca attcggtaat attgaaatat tcagccagtt tcagagcgaa
     7741 ttcgtgaaaa aaatcggctt ctgatgccgg tattctgtta ttttccgtga gataagtgaa
     7801 tatattcttt accggactgg aaactgaacg tattgccttt ttaaaaacct ctgatttatt
     7861 tggagtgagc gttttttgta atgcgtggat gtctgagcta tacaacaatg tcccgtgatg
     7921 caattctctg tttcttgaaa tatgtgaagc cgtccccgat accttaaaat tatccagcca
     7981 caggtctttg cgtttacctg cttcaacagg tatattcatc gagtgaagga catttatgac
     8041 tggcttcaaa aagccggcac tcagggaaga ctgttcggat ctcttattgt gaataaaaca
     8101 gaagttcagg tttccctcat cgtgatacac agtgccacca cccgataaac gcctgacaat
     8161 ttgaatatta ttctgaaaac agaaacccat attcacttca ttgctcaccg cctgattgca
     8221 accgatgacc acacttggct gatttacata cagaaagaga tattcttctg atttatcaga
     8281 aaataaatat tcttcggttg ccaaattaaa cgaaggcgaa gtggatgtgg aaaaaagaat
     8341 tttcatgggt ctgcctctta atccccttca atgggaactt tttacagaat taatttttca
     8401 gatgattgtg tgacaaatta ataatccaaa tcatcacaaa attagcaaaa acgcccgatt
     8461 gaaaaaatca accggacgct atatctcaaa tctccactga gggaaatttt ggagttcgtt
     8521 acttcacatt gaacgctaca cgaagttcgt ctatttcctt atcgcccatg ggtttcagtt
     8581 cgccaatgga ggttgccata accagcgaat tggcagagaa ttcggcaact tcaagataat
     8641 caaacgtgtt gagcaattta tcgccggtga caaaaacgct gtcgttttca accaggatca
     8701 cccggttatc ggcaaacatc tgagccactt catctatttc tttgtaaata tttccgaacg
     8761 gaatggatgg aacatcctgc aggaaaatcc agctttcggg tatagtgcgc acatcgaatt
     8821 ttttgccgct cacggcatgt gccatcagat taaccggttg tgtgcagatg attgaattga
     8881 tatgtgggtt caactgataa atccgctgat gcagcgccac cgaacggctt ggagtttttc
     8941 cggcttcagc cattccgttt tttatttgta caatatcttc tggcataatg tcccaacgtg
     9001 ccacatcgcg gggtgtaatc aggaaatcgc catctttcca tcttacagag actgttccat
     9061 aagtagaaat catcagtccc tgattacagg cacggcgtat aatcttcacc atatcagaac
     9121 gtaatgcccg ttcatcggaa ggatacgaaa tatccataaa atgaggaaca ttggatggaa
     9181 tatgatgaac atattttgat acttcctcat ctgtcagata gtttacaccg ccaagttttt
     9241 tggcattgat gatggtacgg caacaaaatt cgagtgtttc gaaccgctga taggcgtcca
     9301 tcatatctgt ccctcccaac accacgccgt gattttccat tatcacagcc ttataactgg
     9361 tatttttaaa ttgtctggcg attttttcgc ccaagtcttc gcttcctgga cagccatatt
     9421 cggcaaaacc aatatcacca cagatattat gcgcctgagg aacaatcttg gtatcgggaa
     9481 cggttcgggc aatactgaaa gccaccaatg caggcggatg tgcatgaatt accgctttca
     9541 actccggtct tgcttcgaaa atggctttgt gaaacggaaa ttccgacgac ggtttgtgag
     9601 gacctaccac cgttccgtct tttttcacac acataatgtc tttggtcgta aggtttccct
     9661 tgtcaacggc ggatggagta atccagatat cgccattttc atctttaatg gagatgttgc
     9721 ctcctgaggt ggtggtcatt ccactgcggt agatacgttc aataatcaca ttgatttgtt
     9781 ctaccggatg catcattttt acatcgagtt gtttcatatt tgcccctaac cccctaaagg
     9841 gggaattatt tgatttatat atttgtaata agatttaaat tgttgatact cgctcccgaa
     9901 gtcccccctt tagggggttg ggggggcaat tttctaacaa atatttttct gcttccacgt
     9961 tccaaagtcc attatacctg ttgcatattt cagccagatt tctgaataaa tcatcggttt
    10021 cacccaatcc tgcaaaatct tcaatagcag tcaaaggcat tgtgatatgg gtatatatca
    10081 atttttttcc accgggaatt tcgggaagat gattcgttgc ttcaatcacc gcatcgagtc
    10141 ctccgatatg ggtaataagt ccggcaggat caagtccttt gctcattatt cggagggctt
    10201 ctaccatatc gtcgttattt cctccactgg taccaacaac atgtgtgtag ccataatgaa
    10261 cattataaaa attaaacttt gcactgaaat taggatcaga aggaccagca aagaaattga
    10321 ggcatccgtc gaatgcaagt attgcatctg cctgctcgac aacaggacca acaggagcaa
    10381 aaacaaatac atcattgtat ccgacaccat ttgtgattgc ttttagttct tcgaccggat
    10441 ttttcatgtt tccggtattg atgtaaatca actgaatacc ccgtaaagct gcggactgaa
    10501 cagtataaag cgttgcggcc cgatcgagac gactttgatc aacatcggtc acaaccacca
    10561 atgagggacg acggtcttca cggtgtaaaa tataattgat agctgccagt cccatcggac
    10621 caactccggc cagaattgcc atttttccac catccacaat ttccattttg tgaatataac
    10681 tgccgggcgt tatgtgataa ttggcatgca tggcacctat cacacaggat aaaggctcag
    10741 ccagagaagc cggataaaat ccctctccgt gataaggtag cagacagtct ttttccatca
    10801 cctcggcggg gataatcacg taggtggcat cacctccaat atattgataa ctgtaaccgg
    10861 gcgcactaag aactccgacc ggtccttttt caaaatttaa cgcgggctga atggaaaatt
    10921 tgtctccggc tttgaattta tgcgaccatt ttgctcccac ttcgaccaac tcgccggcaa
    10981 actcgtgccc gatgatgatg ggattttcag ctacatcctt cggaattcgt ttgtgatcag
    11041 ttccctgtgt tgaagctttg tacgacgaca tacacaacga gtccgaaaca actttggcta
    11101 aaatttcatt ctctttaatc tgcggcaact caaactcttc catccgaaga tcttttttgc
    11161 catataagcg gattgctttt gttttcataa tttatctgcc ccctaaatcc ccctaaagga
    11221 ggacttgtgg aagactcatt tatttattgt tacttttttt atcattcccc ctttaggggg
    11281 ttagggggct tttttaattc agcatcacct gccctccgct aattggcaat gcttgtccgg
    11341 tttcgccgca ttgttccatg agatacagca cacccttggt tacatcttcg ggtgtacagc
    11401 cttttcgcat cggaacctgc gacaaataaa acgcttttac atcgtcgatg gtttttgctc
    11461 cgggcacttt tcctgcattc agatactgaa cgaaaagtcc gttaacgggg tcgctccaga
    11521 gtggcccatc gtagaaattt ccgggacaaa ttgaatttac ctttattctg aaggaagcca
    11581 gttccagggc aaatgactgt gtcaggccaa tacctccgaa ttttccgccg gcataagcga
    11641 agttggcttt actgccccgc agaccggatt ttgaatttat ctgaataata tctgcaaaat
    11701 aattttctga tgcatatttc gtttgcagtt tcattacctt gctcactact tttgtgcagt
    11761 agaagtaagc attgtaattt attttagtta cgagatcgaa attttcgggt gtcatgtctt
//
LOCUS       DAOPYB010000090        11771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6191_length_11771_cov_8.824855, whole genome shotgun sequence.
ACCESSION   DAOPYB010000090 DAOPYB010000000
VERSION     DAOPYB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11771
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6191_length_11771_cov_8.824855"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..2707)
                     /locus_tag="PKH58_08015"
     CDS             complement(86..2707)
                     /locus_tag="PKH58_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX89010.1"
                     /translation="MKHFISILLLTFLFAGVSAREQYELKSGWQCQNMKQVVVTGEQL
                     SQSEFALNGWKAATVPGTVLTTMLNNKEVPDPFYGINNKKIPDIYYTGRDYYTYWFVN
                     DFQESPAKGIDQVYLNFRGVNYACDIFFNGKKVNAQRHYGMYLRQSYNITSLLAKSGN
                     NRLAVIVYPVDPVGEPNGGQGGDGRIAKNLSHQYAAGWDWIQPVRDRNTGIWDKVTIE
                     KTHGVALKNPHVITIVPGKRDPEAKQQAPAIVKATVEVENPQAKSIRGTVEYTIDGQT
                     VKQPVVLAASGITEVKLPDMTLKNPKLWWPNMYGDHPLYDLTYRFVSADGKVLDNTDV
                     KVGVREITHTWNERTRSMQFAVNGQKIFIKGGNWIISDAMLRFTPERYDAEVRFHRDM
                     NLNLIRIWGGALIERPEFYEACDKYGMLVFQDFWFSGDCNGRWVDPRKKDDQWTRRQY
                     PDDHALSLLSMADMIKMVRNHASLAIWCGGNEITPPADIIIPLQDSILPKLDGTRCFF
                     KYSNTDSMSYNSIGGNGDGPYTIQDPKTFWTYRTFPFNSEVGSVGTGDAESLERFLPV
                     ENQVVPQYLQNKIDSVWDYHKDIGYGNFIASYGNPENIKEFGDIAQLVNFDQYRALAE
                     GFSAHAWDWYTGFIIWKTQNPWTALRGQMYDYYLDVNACLYGLRNGSEPLHIMCNPVD
                     GMAMIVNNTFHSYHDMMLKTSFIDLDGKEIPANDMIVEISPTSTQQYFPLKRDMDKLR
                     AKNGVFLKMQIVDAQQTVKSENIYWLPDSTGNYSGLQHIMKANVIVTARKVSSNTIEV
                     KIANPSGNPLAFFNRVSLVDSATGKRVMPVFYDSNYVSVLPGSDKIIRLEYNGIVDGK
                     VTVEGWNVPLQTIPVRN"
     gene            complement(2774..5026)
                     /locus_tag="PKH58_08020"
     CDS             complement(2774..5026)
                     /locus_tag="PKH58_08020"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX89011.1"
                     /translation="MMKKSLLFIILLFVVFQNGDARLKDLAQYVDPYIGTGDHGHVFL
                     GAHVPFGAVQVGPNNYVKGWDWCSGYHYSDSIVTGFSQMHLSGTGIGDLGDILIMPYT
                     GKLMTSPGTVKTPLSGWATLYTHKDEVVKPGYYSVNLKAYSIKAELTATERVALHKYT
                     FPQSSSAHIGINLAMGIGWDRPVKTFIKKVDATTYTGYRYSTGWSVDQRLYFAIKLSR
                     AADATELFNGKRPQKGIELDGDSAVAILSFKTKAKEVIMVKVGVSPVSESNALDNITA
                     EIPAWNFQQVADNAYAKWNKELGRVSVESKDIHQLRTFYTALYHLFTAPVLFNDHNGD
                     YRGTDKKEYKNPGFQNYTIFSLWDVYRAAQPLYTIIQPDRVPDMVQSLLAIYKQQGKL
                     PIWPLMGSETDCMVGYPAVPVIADAYFKGFKGIDPELAFEAMKASSMRDDYGVNHLKT
                     RGYIPADKEKESVSKALEYALSDWAIAQLAQKLGKQSDYEYYSKRAKAYTQYFDPRTD
                     FMRPKLDNGSFREPLDPFKSIHEWGDYTEGNAWQYTWLVPQDVEGLGNLFGGEKPFVR
                     KLDSLFVVQGGLGAEASPDISGLIGQYAHGNEPSHHIAYLYTYVGQQWKTAEKIRYIL
                     ENMYFDKPAGLSGNEDCGQMSAWYILSSLGFYQVNPAGACFVFGSPSFDKATITLPAG
                     KKFIIDVHNTGSTNIYIQSVKLNGQAYSHSFITYKNIMAGGTLEFVMGDKPNKLFGVN
                     EQDRPKSKVY"
     gene            complement(5066..6541)
                     /locus_tag="PKH58_08025"
     CDS             complement(5066..6541)
                     /locus_tag="PKH58_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HNX89012.1"
                     /translation="MTSRRDFLRNSAMVFAGLAINKSVFGNLLTPASSSKVSSLQPSD
                     ITYVSNRPILAKRNFTSKAVEEIIHQIKQKIKNPKLAWMFENCYPNTLDTTVEFEIKN
                     GRPDTFVITGDIEAMWLRDSSAQVFPYLPLASKDSQLSLMIEGIIRRQSWCINLDPYA
                     NAFNKGATGSEWDKDHTKMIPELHERKWEIDSLCYPIRLAYYYWKKTGNTSMFDESWK
                     SAMKRVYETFVEQQRKKGTMDTSYSFTRDTDKQSDTVLNKGFGSPVNPVGLICSSFRP
                     SDDSTLFSFLIPSNLFAVESLNQLAEISNKVTGDVSFGSKCKALAREVFQAVNKYGIV
                     THSKYGKVYAYEVDGFGGHVFMDDANVPNLLGLPYLCSFIKVSDPVYINTRRLVLGSD
                     NPYFFKGKAAAGVGGPHVGLDWIWPMSFIMQALTSTDEHEIGECIQTLLRTDAETGFM
                     HEAFHKDDPMKFTRPWFAWANTLFGELIIKVASEHQSLLAV"
     gene            complement(7082..9094)
                     /locus_tag="PKH58_08030"
     CDS             complement(7082..9094)
                     /locus_tag="PKH58_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX89013.1"
                     /translation="MLTSGKSSDEPPRPYGAIPSESQLEWQKMEYYMFVHFGPNTFTD
                     VEWGNGKEDPKVFNPAALDCRQWAATAKAAGMKGIIITAKHHDGFCLWPSKFSTHTVR
                     ESGWKNGQGDVLRELSDACKEAGLKFGVYLSPWDRNHPSYGTPEYNQLFANTLNEVLS
                     GYGDVFEQWFDGANGDGTKRQVYDWSLFHNVVYKNQPQAVIFSDIGPGCRWMGNEQGI
                     AGETNWSRLNIKGFEPGSKAPSLDTLNRGNIAGTDWVPAETDVSIRPGWFYSPSTNDK
                     VKSLEQLTEIYFNSIGRNSNLLLNVPADRSGRIHSGDSIRLMEFRKSLEAIFRTNLAS
                     NGIVTATNTRGKSIRFSPANILDNKYDTYWATDDNVHTASLVVTMKKSQTFNRLLIQE
                     YIPLGQRVESFNLEYWDNLTKSWRALSQGTTIGYKRILRFADVTTTKVRINILNSLAA
                     PVINNIGFYFASAQLSAPTVERTMQGDVSLRSEAAGAAIYYTLDGSLPSATSTVFLQP
                     FALPNGGIVKAIAMTHNGAVQSEVVTVNYDIAPAQWKVIAPEVANVAKVIDGDTSTSV
                     VLSQQDALAVDFGKILPLKGFFYVPVNDVNAANLFRYNCSVSIDGQTWTKLINNFIFN
                     NIKNNPIRQDVLFDQPVQAKYIRIEPLESVNSTRYYAVSELGILSR"
     gene            complement(9206..11071)
                     /locus_tag="PKH58_08035"
     CDS             complement(9206..11071)
                     /locus_tag="PKH58_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNX89014.1"
                     /translation="MLCMSGYTANAQQKHLFEVKDGSFMYDHKPMQILSGEMHYARIP
                     HQYWHHRLKMLKAMGLNTVATYVFWNAHETEPGKWNFEGDRNLSEFVKAAGEEGLFVI
                     LRPGPYACAEWEFGGYPWWLQNCKGIELRRDNPVFLKYTQLYINRLYQEIGQQLITKG
                     GPIIMVQVENEFGSYVSQRKDIPIDEHRVYNAKIKDQLLSAGFDVPLFSSDGSWLFEG
                     GTIDGILPTANGEGNISNLKKAVDKYHGGKGPYMVAEFYPGWLSHWGEPFPNISATSI
                     ARKTEEYLQNGISFNLYMAHGGTNFGFTSGANYDKTHDIQPDLTSYDYDAPISEAGWV
                     TSKYDSLRSVIRKYVHIKLPPVPASIPVIEIPAVKLTRVADVLSYAEKMPAVTSETPL
                     TFEQLNQGYGYILYTRHFNQPISGTLEVAGLRDYAVVYVNGEKVGELNRNTKHYTLDI
                     DVPFNATLEILVENMGRINYGAEIVHNTKGIISPVRINDMEIVGDWSMYKLPMDKCPD
                     LSQYAKTDVYENETVKIQSLKGKPIVYEGSFVLNEVGDTFIDMSNWGKGIVFINGNNI
                     GRYWKVGPQQTLFIPGVWLKKGENKISVFEQINDVFQTDVRAVKSPVLNMVKVNN"
BASE COUNT         3498 a         2568 c         2132 g         3573 t
ORIGIN      
        1 aacgaatctt caaacatttg ccaatctggt ttatttgctc aatatacatt cgatgtttat
       61 ggcttatcct tttctttttt atctgttaat ttctaaccgg aatagtttga agcggcacat
      121 tccaaccttc tactgttact tttccatcca cgatgccatt atattctaaa cgtataattt
      181 tatcagaacc cggaagtacc gacacataat tactgtcata aaacaccggc ataacgcgtt
      241 ttccagtggc actatcaaca agggaaactc tattaaaaaa tgccaaagga ttgccggaag
      301 gattggcaat tttaacctcg atcgtatttg aagaaacttt gcgagctgta acgattacat
      361 tcgctttcat tatatgttgt aatcccgagt aattaccagt tgaatcgggt aaccagtaga
      421 tattttcact ctttacagtc tgctgagcat ctacaatctg catcttgagg aatactccat
      481 tttttgcacg cagcttgtcc atatctcttt tcaaagggaa atactgctga gttgatgttg
      541 ggctaatctc aacaatcata tcattagccg gaatttcttt tccatcaaga tcgataaacg
      601 aagttttaag catcatgtcg tgataagaat ggaaagtatt attcacaatc attgccattc
      661 catctaccgg gttgcacatg atgtgcagag gttcacttcc attacgaaga ccgtaaagac
      721 aagcatttac atcaagataa taatcataca tctgtcctcg caaggctgtc catggatttt
      781 gtgttttcca gattataaaa ccggtgtacc aatcccaggc atgagcacta aaaccttctg
      841 ccaaagcacg atactgatcg aaattgacca actgagcaat atcgccaaac tctttgatat
      901 tctcaggatt gccataagat gcaataaaat tgccatagcc aatgtctttg tgataatccc
      961 atacggagtc aattttgttt tgcagatatt gcggaactac ctgattttct actggaagga
     1021 aacgttcgag tgattcggca tcgcccgttc ctacagagcc cacttcggag ttgaatggga
     1081 aagttcgata agtccagaaa gtcttaggat cctgaatagt gtaaggtcca tctccattac
     1141 ctccgataga attataagac atgctatcgg tatttgaata tttaaagaaa caacgagtac
     1201 catccaattt aggtaaaata gaatcctgca atggaataat aatgtcggca ggtggggtta
     1261 tttcattacc tccacaccag atggccagtg aagcatggtt acgtaccatc ttgatcatgt
     1321 cagccattga aaggagcgat aaagcatgat cgtcgggata ttgacggcga gtccactgat
     1381 cgtctttttt cctcgggtcc acccaacgac cgttgcaatc gcctgagaac caaaaatcct
     1441 ggaacacaag cataccatat ttatcacaag cttcgtagaa ttcaggacgt tcaatcagcg
     1501 cccctcccca gatacgaatc aggtttaagt tcatatcccg atgaaaacga acttcagcat
     1561 cgtaacgttc gggagtaaac cgcaacatgg catcggatat aatccaatta ccccctttta
     1621 tgaatatttt ctgcccattg acagcaaatt gcatactgcg ggtacgttcg ttccatgtat
     1681 gtgtaatttc acgtacacct acttttacat ctgtattgtc caatactttg ccatcggctg
     1741 acacgaaacg ataagtaaga tcgtagagcg gatgatcgcc atacatattc ggccaccaga
     1801 gttttggatt tttcaatgtc atatcaggaa gtttaacctc cgtgattcct gaagctgcaa
     1861 gcacaacagg ctgcttaact gtttgaccat ctatggtata ttccacagtt ccacgaattg
     1921 attttgcctg cggattttcc acttctaccg tagctttgac aattgccggt gcttgttgct
     1981 tagcttccgg atcacgcttc ccgggaacaa tggtaatgac atgcggattc ttcaacgcaa
     2041 caccatgtgt cttttctatc gttaccttat cccaaatacc ggtatttcga tcgcgaacag
     2101 gctgaatcca gtcccagccg gcagcatact ggtgcgacag attttttgca atgcgtccat
     2161 caccaccctg accaccattc ggttctccaa ccgggtcaac gggataaaca atcaccgcca
     2221 gacgattatt cccgctttta gctaacagcg aagtaatatt gtaagactga cgaaggtaca
     2281 ttccataatg acgttgcgcg ttcaccttct tgccattaaa aaagatatcg caagcatagt
     2341 taacgccgcg gaagttaaga taaacctgat caataccctt tgccggtgat tcttgaaaat
     2401 cgtttacaaa ccaataggta tagtaatccc gacctgtata ataaatatca ggaatttttt
     2461 tgttgtttat cccataaaat ggatcaggca cttccttatt attcagcatg gtagtaagaa
     2521 ccgttcccgg tacggtagca gctttccaac cattcaacgc aaattccgac tgactgagtt
     2581 gctcgcctgt cactaccact tgtttcatat tctgacattg ccatccgctc ttgagttcat
     2641 attgctcccg agccgatact ccggcaaata aaaaagtaag aagtagtatt gatataaaat
     2701 gcttcattag attgattttt tattgtgtgt cagttgaacc aacactaaaa gtgacagcgg
     2761 acagattatt cgtttaataa acctttgatt ttggcctatc ctgctcgtta actccaaata
     2821 atttgttcgg tttatcaccc attacaaatt ctaacgtacc accggccata atatttttat
     2881 aagttataaa tgaatgagaa taagcctgac cattcagttt caccgactgg atataaatat
     2941 tggttgaacc tgtattatgc acatcgatta taaatttttt acctgccggc aaagtaatag
     3001 tggccttgtc gaacgaagga gaaccaaata caaaacaagc tccggccgga ttaacctgat
     3061 aaaagcctaa agacgaaaga atgtaccatg ctgacatctg gccacagtct tcgttcccac
     3121 tcagtcctgc cggtttatca aagtacatgt tttcgagaat ataccgaatc ttttcagctg
     3181 ttttccattg ttgccctacg taagtatata aataggcaat gtgatgactc ggttcattac
     3241 catgagcata ttgaccaatt aaacccgaga tatcaggtga tgcttctgct cccagacctc
     3301 cctgaacaac aaacaggcta tcgagtttcc gaacaaacgg tttttctcca ccaaacagat
     3361 tcccaagacc ttccacatcc tgaggaacta accaggtgta ttgccatgca tttccttcgg
     3421 tataatctcc ccactcatga attgacttga acggatcaag cggttcgcgg aatgagccgt
     3481 tgtcgagctt tggacgcata aaatcagtcc tcggatcgaa atactgtgta tatgcttttg
     3541 cacgttttga atagtactca tagtcacttt gtttgcccag cttttgagcc agttgagcaa
     3601 ttgcccaatc ggacagtgca tattccaatg cttttgaaac cgattccttt tctttatctg
     3661 cgggtatata gcctcttgtt tttaagtgat taacgccata gtcatctcgc atagaagatg
     3721 ctttcattgc ttcaaatgcc aattcggggt caatgccttt gaaaccttta aaatatgcat
     3781 cggcaataac aggaactgca ggataaccaa ccatgcaatc tgtttctgaa cccatcaaag
     3841 gccagatagg caatttacct tgttgcttat aaatagcaag taaagattgc accatgtcag
     3901 gaacgcggtc gggctgaatg atggtataaa gaggctgtgc tgcacgatat acatcccaca
     3961 acgagaaaat ggtatagttt tggaatccgg gatttttata ttctttcttg tctgtgcctc
     4021 tgtaatctcc attgtggtcg ttgaacaaaa caggagcagt aaacaaatga tacaaagcgg
     4081 tatagaatgt tctcagctga tgaatatctt ttgactcaac agaaacccga cctaattcct
     4141 tgttccattt tgcataggca ttatcagcta cctgttgaaa attccatgca ggaatttccg
     4201 cagtaatatt atccaaagca tttgattcgc ttacaggcga cactccgact ttgaccatga
     4261 ttacttcttt ggctttagtt ttaaaactca atatcgccac agcagaatct ccatccagtt
     4321 caattccttt ttgtggcctt ttaccgttaa acagctcagt tgcatcggct gcacgcgaga
     4381 gtttgatagc aaaatataaa cgctgatcta ccgaccaacc tgtggaataa cggtatccgg
     4441 tataagtagt agcatccacc tttttgatga aggttttaac cggtctatcc caaccaatac
     4501 ccattgccag attgatgcct atgtgagccg atgaagattg cggaaaagtg tatttgtgta
     4561 gcgcaacacg ctctgtagct gtcagttctg cttttatcga ataagcctta aggttcacag
     4621 aataatagcc gggcttaacg acttcatctt tatgtgtata tagcgtagcc caacccgaca
     4681 aaggagtttt cactgtaccc gggcttgtca tcagttttcc tgtatatggc attatcagga
     4741 tatcacccaa atctccaatg ccggtaccac taagatgcat ttgcgaaaat ccggttacta
     4801 tactgtcgga ataatgataa cccgagcacc aatcccagcc ttttacataa ttattaggcc
     4861 caacctgaac cgcaccaaag ggaacatgag ccccaagaaa cacatgaccg tgatcgcccg
     4921 ttcctatgta cggatcaaca tactgtgcaa gatctttcaa tcgtgcatcc ccgttttgaa
     4981 agactacaaa cagtagaatt ataaatagca aacttttctt catcattttg tagatattat
     5041 tgaatttaac ttatttgaat ttttcttaaa cagccaaaag cgattgatgc tcactggcaa
     5101 ctttaataat cagttctcca aacaatgtat ttgcccaggc aaaccacggt cgagtgaatt
     5161 tcatgggatc atccttatga aaagcttcgt gcataaatcc tgtttcggcg tctgtgcgta
     5221 aaagcgtctg aatacattca ccaatttcat gttcatccgt agaagtaagt gcctgcatga
     5281 taaagctcat tggccatatc caatcgagac ctacgtgtgg accacctacg ccggcagctg
     5341 ctttcccttt gaaaaaatag gggttgtcgc tgccaagaac cagacggcgt gtgtttatat
     5401 aaaccggatc agacaccttg ataaaagaac aaagataagg taaacctaat aagttaggca
     5461 cattggcatc gtccataaaa acatgtccgc caaaaccatc cacttcgtat gcatatactt
     5521 ttccgtattt tgagtgagtg acaattccat atttattgac cgcctgaaat acctctctgg
     5581 ctaaagcctt acatttggaa ccaaaactga catcaccggt aaccttattg gatatttcag
     5641 ccaattggtt cagcgattcc acagcaaaca agttagacgg tatcaagaag ctaaaaagcg
     5701 tagagtcgtc cgacggacga aaagaagaac atattaaacc aaccgggtta accgggcttc
     5761 caaatccttt attgagaact gtatcgctct gtttatcggt gtcacgggta aatgaatagg
     5821 aggtatccat tgttcccttt ttgcgttgct gttccacaaa agtttcataa acgcgtttca
     5881 tggcactttt ccagctctcg tcaaacatgg atgtatttcc ggtttttttc caataataat
     5941 atgccaaacg aatcggataa cacagcgaat cgatttccca cttgcgttcg tgcaattcgg
     6001 gaatcatttt agtatgatcc ttatcccatt cacttccggt agctccttta ttgaaagcat
     6061 ttgcatacgg atcaagattg atacaccaac tttgacgacg gataatccct tcaatcataa
     6121 gactcaactg actgtctttc gaagctaaag gcaaatatgg gaaaacctgt gccgatgaat
     6181 cgcgcaacca cattgcttct atgtcgccag tgataacaaa ggtatcagga cgaccgttct
     6241 ttatttcaaa ttccactgtt gtatccaatg tattcggata acagttttca aacatccatg
     6301 ccaatttcgg attcttgatt ttttgcttaa tctgatgtat aatttcctcc actgcttttg
     6361 aagtgaaatt gcgttttgcc agtatcggac ggttgcttac ataggtaatg tcagacggtt
     6421 gtaaagacga taccttcgaa gatgatgccg gcgtcaaaag atttccaaaa acacttttgt
     6481 ttattgccag ccctgcaaat accatcgcgc tgttacgtaa aaaatctctg cgtgaagtca
     6541 taattattta agattaacat tagtgattct ataagtttga aattttgaaa tgatattttt
     6601 ttgctgaatg taaaggttgt taagaaaaac ttttttgttt ttactttttt actttgcgaa
     6661 aagttgatga cattagaata agtataatta acctgtagtg cctttagaac aaagtaataa
     6721 aaagttgttc gcttcggata aaatccgtat atttaattga ttaacaatcg attaaatatg
     6781 acgaacaact tcatcacaaa gtactagaaa attctcgaaa tattaaaaca atttgagtca
     6841 tgaagtaact ttataattaa attcgttgtc cgcgtttgag ctatattgaa ttaattgctg
     6901 ttgattttac tgcaaaatct taaagtattg atactgaata tcaattattt agaattctgc
     6961 caggttctct aagttcacgc attgaacaaa gtgtatatta tcgtcggaga agaaaatcat
     7021 tttctgttaa agagctaatc ggcaaagcga tgtcggataa ataaaccgaa actgacccga
     7081 ttcaacgaga aaggatgcct aattcactca cagcataata tcgggtacta tttacagact
     7141 ctaatggttc aattctaata tattttgctt gaacaggttg atcaaaaagc acatcctgac
     7201 gaataggatt attctttata ttattgaata tgaaattatt tataagtttc gtccatgttt
     7261 gaccatctat actaactgaa caattgtaac gaaaaagatt agcagcattc acgtcattta
     7321 caggcacata gaaaaatccc tttaaaggca atattttccc aaaatccact gccaatgcat
     7381 cttgttgact cagtacaacc gatgtagatg tgtcaccatc aattactttc gctacattag
     7441 ccacctcagg agcaataact ttccattgag ccggggcaat atcataatta acagtcacca
     7501 cttcactttg aacagcccca ttatgcgtca ttgcaatagc cttaactata cctccattgg
     7561 gcaaagcaaa aggttgcaaa aagactgtcg aagttgcaga aggcagtgat ccatccaatg
     7621 tgtaataaat ggcagcacca gcagcttcac ttcgtaggga aacgtcacct tgcatagttc
     7681 gctctacagt aggtgccgat aattgagcag aagcaaaata aaatcctata ttatttatta
     7741 ccggagcagc taatgagttt aaaatattaa tgcgcacctt tgttgtagtt acatctgcga
     7801 atcgcagaat gcgtttataa ccaatggttg taccttgcga caatgcacgc caacttttgg
     7861 ttaaattatc ccaatactcc aaattaaaag attcaacacg ctggcccaaa ggaatatatt
     7921 cttgaatcaa caaacgatta aaggtctgtg actttttcat ggttaccacc aatgaagctg
     7981 tgtgtacatt atcgtctgtt gcccaataag tatcgtactt gttgtcaaga atatttgccg
     8041 gcgaaaatcg aattgatttt cctcttgtat tagtggctgt tacaatacca ttactggcaa
     8101 gatttgttct aaaaatagct tctaacgact ttcgaaattc catcaggcga atagagtctc
     8161 cggaatggat acgtccgctg cggtcggcag gaacattaag caacaaatta ctgtttcgtc
     8221 ctatgctgtt aaaataaatt tcagtaagtt gttcaagtga ctttacttta tcattggtag
     8281 aaggactata gaaccatccc ggacgaatgg aaacatcagt ttcggcaggc acccaatcag
     8341 tccccgcaat atttccacgg tttaatgtat caagagaagg cgctttgctg cccggttcaa
     8401 atcctttgat gttgagacgc gaccaattgg tttctccggc aattccttgt tcgtttccca
     8461 tccaacgaca accgggtcca atatcactaa aaataacagc ctgaggttga tttttatata
     8521 ccacattgtg aaataaagac caatcatata cttgccgttt tgtgccatcg ccatttgccc
     8581 catcaaacca ttgttcgaat acatcaccat agcccgatag gacttcattg agtgtgttgg
     8641 caaatagctg gttgtattcc ggagttccgt aagatggatg gttccgatcc cacggagaca
     8701 aatagactcc aaactttagc ccggcttctt tacaagcatc cgaaagttct ctaagcacat
     8761 ctccctgacc gtttttccaa ccactttcgc gaactgtatg tgtactgaat ttacttggcc
     8821 ataagcagaa accatcatga tgcttagctg tgataataat acctttcatt ccggcagctt
     8881 tggctgtagc tgcccattga cgacaatcta aagcagcagg gttaaacact ttcggatctt
     8941 ctttcccatt tccccattct acgtcagtaa atgtattagg accaaaatga acgaacatat
     9001 agtactccat cttttgccat tccaactggc tttcagaagg tatggcccca tagggacgag
     9061 gtggttcatc actactttta ccacttgtca gcactattaa gaatgcaatc aatattatat
     9121 tatttctcat attatataat tattcctcat catttagaac acaatactat ttgctcatcc
     9181 gttgtagaaa acgaaaatct cctatttagt tgttaacttt caccatattc aacaccggac
     9241 tttttacggc tctcacatcc gtttgaaaaa catcgttaat ttgctcgaaa acagaaattt
     9301 tattctctcc ctttttaagc catactccgg gaataaaaag agtttgttga ggaccgactt
     9361 tccagtaacg tccaatatta tttccattta taaaaacaat accttttccc caattactca
     9421 tatcaataaa ggtatcgccg acttcattaa ggacaaatga tccttcgtat acaattggct
     9481 tcccttttaa agattgaatc ttaacagttt cgttttcata cacatcagtc tttgcatatt
     9541 ggcttaaatc aggacatttg tccataggta atttatacat tgaccaatca ccgacaatct
     9601 ccatatcatt aatcctgacc gggctgataa tacccttggt gttatgcaca atttcagcac
     9661 cgtagttgat acgtcccata ttttctacaa gaatttctaa tgtagcatta aaaggcacat
     9721 caatatcaag ggtgtaatgt ttggtattcc ggtttagctc acccactttt tctccattaa
     9781 catacacaac tgcataatcg cgtagaccgg caacttctaa tgtcccgctg ataggttgat
     9841 taaagtgtcg tgtataaaga atataaccat atccctgatt caattgttca aaggtcaaag
     9901 gggtctcact tgttacagca ggcatttttt cagcatagct caatacatca gccacacggg
     9961 ttaatttcac cgcaggaatt tcaataaccg ggattgaagc cggaacaggt ggcagtttaa
    10021 tgtgtacata tttacgaatg acggagcgga gagaatcgta tttagatgta acccaaccgg
    10081 cttcacttat aggtgcatca taatcatagc tggtaagatc gggctgaata tcgtgtgttt
    10141 tatcataatt tgcaccactc gtaaaaccaa agttagttcc accatgtgcc atgtataaat
    10201 taaacgagat accgttctga agatactctt cggtcttgcg ggcaatactc gttgcactga
    10261 tattcggaaa aggctctccc caatgcgaca accagcccgg ataaaattcg gcaaccatgt
    10321 aaggtccctt tccaccatga tatttatcca ccgccttttt cagatttgag atattaccct
    10381 caccattagc ggtcggcaaa attccatcaa tagtacctcc ttcaaataac caggaaccat
    10441 cggaactaaa cagaggtaca tcaaatccag cagacaaaag ctgatctttg attttggcat
    10501 tataaacccg gtgttcatcg attggaatat cttttcgttg agatacatac gatccaaact
    10561 cattctctac ctgtaccata ataataggac caccctttgt gataagctgc tgaccaatct
    10621 cctgataaag gcgattgatg tatagctggg tgtattttaa gaatacagga ttgtcacgtc
    10681 tcaactctat tcctttgcaa ttttgcaacc accagggata tccgccaaac tcccattcgg
    10741 cgcaggcata aggacccgga cgaagaataa caaaaaggcc ttcttcccct gctgctttta
    10801 caaattccga caagtttcta tctccttcaa aattccactt acccggttct gtttcatgtg
    10861 cattccaaaa cacataagta gctactgtat ttaatcccat agctttgagc atcttcaggc
    10921 gatgatgcca gtactgatga ggaatacgag cataatgcat ctcgcccgaa aggatctgca
    10981 tcggtttgtg atcgtacatg aaacttccat cctttacttc gaaaagatgt ttttgttgag
    11041 catttgctgt atatcccgac atgcatagca ttgctacggc aaggatgagt tttctgttta
    11101 gtttctgtaa catatctatt ttttttattt tctagttgaa tagctattta tattgagaga
    11161 aaacctgaaa cattgttcta ccctcaatct gtaatttaac tctttctaaa ttgcttcctg
    11221 attaggaaaa tagttgcatc ttcaatgtgt gctcctgcaa gcgaatctct ttcgaaataa
    11281 tagatccaat gtagttcagg aggaactttg accagattga gatcaatcca gtattttatg
    11341 caaacatagc agattttatt gttaattgct actcaacaat aattttctgt tataatttca
    11401 attagtaatg caactttgat ggcaggatag tatagtttat tttttcagag ccagggagga
    11461 aggcctcccc cctttgctcc gatggaaaaa aatgactata attgttctcc gccaaaagtt
    11521 tacatttatg agtcatttaa ccagggaaca aagatatact attgcatcga tgttgctaaa
    11581 cggagacttg cttatgatgc ctgttacggg tctgttggga attactccaa tacttcaata
    11641 ttttagcctg aatgttggat tggaattttt acaaggtgtt attcttaaga ccattgctgt
    11701 acttgtcacc atgttttgta cccgaattaa atggttttgg tgaacgtaaa cgcaaattgg
    11761 aaccgtagat t
//
LOCUS       DAOPYB010000091        11744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6203_length_11744_cov_9.942767, whole genome shotgun sequence.
ACCESSION   DAOPYB010000091 DAOPYB010000000
VERSION     DAOPYB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6203_length_11744_cov_9.942767"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1363)
                     /locus_tag="PKH58_08040"
     CDS             complement(116..1363)
                     /locus_tag="PKH58_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89015.1"
                     /translation="MKNNVVLVLLVLAATTVLAQQPKTNKGIFKTYEPGYFQNTILKG
                     IEEFESSKEAPPAKKTFKADPSDYNIPTDKNAFKQYWHNEPVSQANTNTCWCFSTTSF
                     FESEIYRLTGQKVRISEMFTVYYEYLERAEAWVKTRGNIYFGEGSEGNAVTKIWKKYG
                     CVPYESYTGYLPGQTFYNHQIMFDELKSYTEFVKKNNLWDEASVLANFRAILDHYMGT
                     PPETVTVSGKTMSPKDYMNNVLKFKPDDYCDVLSYMQQPYWQQVEYEVSDNWWHNKDY
                     YNIPLDDYMSVIKNALKKGYTVAIGGDVSEAGFLAREANAAVVPAFDIPSSAIDENAR
                     QFRFSNETTTDDHGMHIVGYLEKDGTTWFLVKDSGAGSRTGGKEKNLNFGYYFFHEDY
                     VKLKMMDCMVHKDMMKEFLPKFK"
     gene            complement(1425..1886)
                     /gene="pyrI"
                     /locus_tag="PKH58_08045"
     CDS             complement(1425..1886)
                     /gene="pyrI"
                     /locus_tag="PKH58_08045"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNX89016.1"
                     /translation="MDKETRELKVTAIENGTVIDHIPANNVFKVIRILNLENNNHQVL
                     FGDNLESKKYGKKGIIKVSNTFFAPDEINKIALVAPTATLIVIRNFKVVEKKQVEVPD
                     EIVKIVKCFNPGCITNHEDIITRFTVIDKNDLKLKCRYCEKITAKNNIVFL"
     gene            complement(1919..2827)
                     /gene="pyrB"
                     /locus_tag="PKH58_08050"
     CDS             complement(1919..2827)
                     /gene="pyrB"
                     /locus_tag="PKH58_08050"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNX89017.1"
                     /translation="MKGKSLISITDYTKDEYLQILNTAAGFEANPNQKILSDKVVASL
                     FFEPSTRTRLSFETAANRLGARVVGFSDAGNTSVQKGESLKDTIIMVSSYADIIVMRH
                     PREGAARWASEVSSVPVINAGDGANQHPTQCLLDLYSIMQTQGTLNNLQIAMVGDLKY
                     GRTVHSLVQAMCNFNATFHLVSPEELKLPSVVKMHIKEANLEYHQYTDLADVIPIADI
                     IYMTRIQKERFSDPIEYEKVKNSYVLNKAMIAGCKPNMKILHPLPRVNEISEDVDSTE
                     QAYYFKQAENGVYVRQALLTTILGAK"
     gene            complement(2943..4286)
                     /locus_tag="PKH58_08055"
     CDS             complement(2943..4286)
                     /locus_tag="PKH58_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNX89018.1"
                     /translation="MRRNVIIIGAAGRDFHNFNTFYRGKAEYNVVAFTAAQIPDIDGR
                     KYPKELAGADLYPDGIPIFAEEDLPKLIKDHDVVDCVFSYSDQPYQKVMAKSALVNAC
                     GANFILLGPRETMVKSTKPLIAVGATRTGCGKSQTSRRIIEILMAKGLKVVAVRHPMP
                     YGDLNAQKVQRFAKVEDLAKHKCTIEEMEEYEPHVVRGNVIYAGVDYEAILRAAENDP
                     DGCDVILWDGGNNDFPFYKPDLTIGVADPHRPGAEVSYYPGEVVARMADVIVINKIDS
                     ADNANIQTVRDNIAKINPKAKVIDGASPLTVDKPELIRGKRVLVIEDGPTLTHGEMRI
                     GAGTVAANKYGAAELVDPRQYAVGKLAETFRIYPNIGTLLPAMGYGEEQTRDLEKTIA
                     NTPCDAVVIATPIDLNRIVKINKPCVKVGYDLQEIGKPDLNEILDDFVAKHGLIK"
     gene            4593..6386
                     /gene="argS"
                     /locus_tag="PKH58_08060"
     CDS             4593..6386
                     /gene="argS"
                     /locus_tag="PKH58_08060"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNX89019.1"
                     /translation="MIEAILVNKVIDAIEKLYGSRAETSSVQLQKTRREFEGDFTLVV
                     FQFTRVSRKSPEATATEIGDYLVANMPEVASYNVIKGFLNLSLQAGYWMHFLNENGDR
                     TDFGFSEVTGGRPVVVEFSSPNTNKPLHLGHIRNNLLGFSLSRILKASGKPVVRVNLV
                     NDRGIHICKSMLAWKKWGNGETPGSSGMKGDHLVGKYYVVFDKHYKAEIAGMVAGGCS
                     PEEAARTAPLILEAQEMLRKWESHDAETIRLWEMMNRWVYEGFDATYKTLGIEFDKTY
                     YESQTYLLGKSLVNEGVVKGVFAKRDDNSVWIDLTNDGLDEKLLLRSDGTSVYMTQDM
                     GTAQQRFEEYKPSQLIYVVGNEQEYHFNVLKLVLKHLGCEWADIIYHLSYGMVELPHG
                     KMKSREGTVVDADDLMEEMFETAERMARELGKLESFEGEEAEKLYRTIGLGALKYFIL
                     KVDPKKNMLFNPEESVDFNGNTGPFVQYTYARIQSVLRNAVKLGIDPESRGSFEMSAE
                     ILPQEKEALRLLHDFPATVSQAADMHSPAVIANYVYELAGAYNRFYHDITILKEENAP
                     ARNFRLGLCNLTGNVIRNAMWMLGIDVPERM"
     gene            complement(6584..6733)
                     /locus_tag="PKH58_08065"
     CDS             complement(6584..6733)
                     /locus_tag="PKH58_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89020.1"
                     /translation="MFATLKYPVLGFIRIRLLIRRECKKWLVELIGSDLQIEVYEDDF
                     VLDRQ"
     gene            complement(8017..8568)
                     /locus_tag="PKH58_08070"
     CDS             complement(8017..8568)
                     /locus_tag="PKH58_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89021.1"
                     /translation="MKRLIIVFAIQISSVLTYGQSLLPLSYISNKAELYCQDLSLQVD
                     NNADLMCRKVLYVSLYDFKNDTFAISLAAIYNHLSLDVNKADYYFEFNNKCIVFNFIN
                     CDAKKIMSKSDFKKLTAIQRTKRRIIESHLLPENIIAGGVIIPRIILISKNQAHELSQ
                     ETYIDVATLPEQFMMFRINDTLH"
     gene            complement(8586..9215)
                     /locus_tag="PKH58_08075"
     CDS             complement(8586..9215)
                     /locus_tag="PKH58_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89022.1"
                     /translation="MKRVFLLLMVLFQSKVYSQPISINETEIDIIISHLNFILDKEPI
                     LSNLNTFFFKIQKEEKTNAYSVAFADMNMADKVPLHNVKCYKNGNRVFYFENDSNINI
                     VRKNTGDIIDPQTIKSITDASFNQNSINDGVLHFPFLFLYKINNHRKANYKVYHPIET
                     SPTKYWIVRGNGKETRIPILEFLDGNGLPNADYLNFLNRNQGSFRIRER"
     gene            complement(9670..10341)
                     /locus_tag="PKH58_08080"
     CDS             complement(9670..10341)
                     /locus_tag="PKH58_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89023.1"
                     /translation="MKRFKIIVIFLVLYSNISLSQEKVIVESFELQQIFSKFLGMPKF
                     SRWSIGRINAIKVITEDNMNTYSVSITGINRDEALSADTYYYYLIDNTYFFLQEDDRI
                     ELKHSKHDSLLFNLSADEKRLFLYKLFRGEGAIISFPVLTLFKVHKKIPILNHRKVTY
                     NTFHPIDQVPKKYWPISPRLYSDTQSIYGGFLDKQNNPNDEYRDLIKKGRFKITVPKT
                     DNNRK"
     gene            complement(10338..11036)
                     /locus_tag="PKH58_08085"
     CDS             complement(10338..11036)
                     /locus_tag="PKH58_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89024.1"
                     /translation="MAGGFSSLSGSATAGIDNYAAEVVIGTVAGGTAAEIGGGKFANG
                     AVTGAFTVMYNHLMHDAFLKLSIKNLEEYKQIENYGRMYNSKAEAVKKAVNITSMIKK
                     ETQIFSLIDSDGNKSYFLEAFINNGEDNATPTNLRCLKSEGYTLVEEDHYSIRDCSDC
                     SSPSYYTVSKNDFIAAQDYQVSVTHHTIGLGSWTIVGPVFLTSFFFPENRADFCNPKY
                     RTDYFNAKYGANKP"
     gene            complement(11268..11615)
                     /locus_tag="PKH58_08090"
     CDS             complement(11268..11615)
                     /locus_tag="PKH58_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89025.1"
                     /translation="MQYNKNDSIFLGFNLNLLGTSITDSIKYISRLYLEDSQKGEYTI
                     YYPFIAAYYIKKVNSKIKGSSIIYFPEKNCPEKERINRLYYPAGTKYYPWKDKINKEF
                     LKKINKQKPLRLK"
BASE COUNT         3220 a         2248 c         2509 g         3767 t
ORIGIN      
        1 gtttgaggcc tgctttccag cgtttttatt ctgttctccg ttttccgttc tccattctcc
       61 attctccgtt tcacctcttg tttgttggta ttgacagata tgaatacctt ccaaactatt
      121 tgaatttagg tagaaactct ttcatcatat ccttatgaac catgcagtcc atcatcttca
      181 gcttcacata gtcttcgtgg aaaaagtagt aaccaaaatt caggttcttt tcttttccac
      241 cggtgcggct gccggcgcct gaatctttga ccaggaacca tgttgtgcca tctttttcga
      301 ggtatcctac gatgtgcatt ccatggtcgt cggtagtggt ttcgttactg aaacgaaact
      361 ggcgtgcgtt ttcatcaata gctgaagatg gaatatcaaa cgcggggaca acagcggcat
      421 tggcttcccg ggccagaaaa ccggcttctg aaacatctcc gccgatggcc accgtgtagc
      481 cttttttaag ggcatttttt attaccgaca tgtaatcgtc aagcggaata ttataataat
      541 ccttgttgtg ccaccagttg tcggaaacct catattcgac ctgttgccag taaggctgct
      601 gcatgtagct cagtacgtcg cagtaatcat cgggcttgaa tttcagcaca ttgttcatgt
      661 aatcctttgg gctcattgtt ttcccgctga cagttacggt ttcgggcggg gtgcccatat
      721 agtggtcaag gatcgccctg aaatttgcta aaacagatgc ctcatcccaa agattgtttt
      781 tcttcacgaa ttcggtgtat gatttcagct catcaaacat tatctggtga ttgtagaaag
      841 tttgccccgg cagatacccc gtgtagcttt cgtaaggaac acagccgtat tttttccaga
      901 ttttagtcac ggcgttacct tcggaccctt cgccgaaata gatatttccc cgtgtcttta
      961 cccatgcttc ggccctttcg agatattcat aataaactgt gaacatctcg gaaatacgga
     1021 ctttttgacc ggtaaggcgg taaatttccg attcaaagaa agaggtggtg gaaaagcacc
     1081 agcaggtgtt ggtgttggcc tgcgaaaccg gttcgttgtg ccagtattgc ttgaaggcgt
     1141 ttttatcggt tggaatgtta taatccgaag ggtcggcttt gaaagttttt tttgcaggag
     1201 gggcctcttt ggatgattcg aattcctcga tgcctttaag aatggtattt tggaagtatc
     1261 cgggttcgta agttttaaaa attcccttgt tggttttggg ttgttgagcc agtacggtgg
     1321 tagcagcaag gactaacagt acaagaacta cattgttttt catagcaacg gttttaagct
     1381 tcagaagata tcaattttct gagaatgata tctgagtttg gtcattataa gaagacgata
     1441 ttattttttg cggttatttt ttcgcagtaa cggcatttaa gcttcaaatc atttttatca
     1501 attaccgtga aacgggtgat tatatcctcg tgattggtga tacaaccggg gttgaagcat
     1561 tttacaattt ttacaatttc gtcgggtact tccacctgtt tcttttctac aactttaaaa
     1621 ttcctgatta caatcagagt agcggtagga gcgaccaggg cgattttatt gatttcgtcg
     1681 ggagcgaaaa aggtattgct taccttgatg atcccttttt tgccgtattt cttgctttcc
     1741 agattatcgc caaacaatac ctggtgatta ttgttttcaa ggttcagaat gcggatgact
     1801 ttgaaaacat tgttggcggg aatatggtcg attacggttc cgttttcaat ggccgtaact
     1861 tttaattcgc gggtttcttt atccatggta aatagtcttt aaatcgttag ttccggattt
     1921 attttgcacc caggatggtc gtcagcagag cttgccttac ataaacgccg ttttcggctt
     1981 gtttaaaata ataggcctgt tcggttgagt caacatcttc acttatttcg ttcacgcggg
     2041 gcaaggggtg aaggattttc atgttgggtt tgcatcctgc gatcattgcc ttgttaagca
     2101 cgtagctgtt cttcactttt tcatattcga tgggatcgct gaacctttcc ttttggatgc
     2161 gggtcatata gatgatatcg gcaatcggaa tcacatcggc gagatcggta tattggtggt
     2221 attccagatt cgcttccttg atgtgcattt ttaccacgct gggcaatttg agttcttccg
     2281 gcgaaacgag atggaaggtg gcgttgaaat tgcacatggc ctgcaccagt gaatgaaccg
     2341 tgcgtccgta tttgaggtcc cccaccatgg ctatctgcag gttgttgagg gtgccctggg
     2401 tttgcataat ggagtacaga tcgaggaggc attgggtagg atgctggttg gcgccgtcac
     2461 cggcgttgat aaccggcacc gacgatactt cgcttgccca gcgggcggcg ccttcgcgcg
     2521 ggtgacgcat cacgatgata tcggcatagc tgctcaccat aataatggtg tccttcagcg
     2581 attccccttt ttgcacgctg gtgtttccgg cgtcgctgaa accaacaaca cgggcgccaa
     2641 gacggttggc cgcagtttca aaactaagcc gggtacgcgt tgatggttcg aaaaacaaag
     2701 atgcaacaac cttatcggat aagattttct ggttgggatt tgcttcgaaa ccggctgctg
     2761 tattgagtat ctgcaggtac tcgtccttgg tgtaatcggt tatggatatc aggcttttac
     2821 ctttcatagg tcaatgatgt tttttgtttc taaagatagt tattcaactt attctctgtt
     2881 gctggaacgg gtgatttttt ggcaaaaaaa aagcggtcag tcgaccgctc ttttttgctt
     2941 ggttatttta ttaagccatg ttttgcgaca aaatcatcaa ggatttcatt caggtcgggc
     3001 ttgccgattt cctgtaaatc gtaaccgact ttaacgcagg gcttgttgat cttcacgata
     3061 cggttcaggt caatgggggt tgcaatcaca acggcatcgc agggagtatt ggcgatggtc
     3121 ttttcaaggt cgcgggtttg ttcttcgccg tatcccattg ccgggagcaa agtgccgata
     3181 ttcggataaa tcctgaaggt ttcggccagt ttgccaactg catattgacg cggatcaacg
     3241 agttcggcag ctccgtattt gttggcggca accgttccgg cgccaattct catttcgccg
     3301 tgggtgagcg tggggccgtc ttcaataaca agcacacgtt ttccgcggat gagttcgggt
     3361 ttgtctacgg taagcggtga agctccgtcg ataactttgg cttttggatt tatctttgca
     3421 atattatccc ttacggtctg gatgtttgca ttgtcggctg aatcaatttt attgatcacg
     3481 ataacatcgg ccatgcgggc aactacttcg ccgggataat aggaaacttc ggcacccggg
     3541 cggtgaggat cggctacgcc gatggtaaga tcgggtttat agaaggggaa gtcgttgttt
     3601 ccgccatccc agaggataac gtcgcatcca tcgggatcgt tttcggcggc gcggaggata
     3661 gcttcataat caacgcctgc ataaatcaca ttgccgcgaa ccacatgggg ttcgtattct
     3721 tccatttctt cgatggtgca tttgtgtttg gcaaggtctt cgactttggc aaagcgctga
     3781 actttttgtg cattcaggtc gccataaggc atcgggtggc gaacggcaac aactttaagg
     3841 ccttttgcca tgagtatctc aatgatacga cgcgatgtct ggctcttacc gcatcctgtg
     3901 cgggttgcac cgacagcaat caacggtttg gtgcttttta ccatggtttc cctggggccc
     3961 agtagtataa aattggctcc gcaggcattc accagggcgc tcttggccat tactttctgg
     4021 tatggctggt cgctgtatga gaatacgcaa tcaactacat catggtcttt aatgagtttc
     4081 ggaagatctt cttcagcaaa aatgggaatg ccgtcaggat agaggtcggc accggccagt
     4141 tctttgggat atttccgtcc gtcgatgtcg ggaatctggg ctgcagtaaa ggctacaaca
     4201 ttgtattcag ccttacccct gtaaaacgtg ttgaaattgt ggaagtcacg tcctgctgct
     4261 ccgatgataa ttacgtttcg tcgcataaaa cttttattta acgggttaaa tttattgatg
     4321 tgatagttgc tgtaattcaa atttcgtgtg acaaagttag tggtttaaac ttgccggcaa
     4381 aattatttat ggttttctta aattcaggag tgatgaagct tatgaaatgt cgggccgtca
     4441 gaatccttta tttgcgcaac ataacgattt gctgcaagct gtatgccact gatagcgtaa
     4501 gatgtgaaag taagacagtc ccaataagca ggttagtaac gggatcaatc gctcaaaaac
     4561 agtacttttg caacactgaa attatcggga aaatgataga agcgattctt gtaaataaag
     4621 ttattgatgc cattgaaaaa ctttatggct ccagggcgga aacatcgtcg gtgcagttgc
     4681 aaaagacacg ccgggaattt gaaggcgact ttacgctcgt cgtattccag tttacccgcg
     4741 tttcacgaaa gtcgccggaa gccacggcga ccgaaatagg agattatctg gttgccaata
     4801 tgcccgaagt agcttcgtat aatgttatca aaggcttttt aaatctttcg cttcaggcag
     4861 gttactggat gcattttctg aatgaaaacg gggatcggac cgattttgga ttttcggaag
     4921 taacaggcgg ccggcctgtt gtcgttgaat tttcatcgcc caacaccaat aaaccattac
     4981 atctcggcca catcaggaac aacctgctgg gcttttcgct ctcaaggatt cttaaggcat
     5041 cgggtaagcc ggtcgttcgc gtcaacctgg ttaatgaccg gggcattcat atctgtaaaa
     5101 gcatgctggc atggaagaag tgggggaatg gcgaaactcc cggatcatcg gggatgaaag
     5161 gcgatcacct tgtcggcaaa tactatgttg tttttgacaa gcattataaa gccgaaattg
     5221 ccgggatggt tgccggcggc tgctcgcccg aagaagctgc cagaacggcc ccgctgattc
     5281 tcgaagcgca ggagatgttg cggaaatggg aatcgcacga tgcggaaacc atccggctgt
     5341 gggaaatgat gaaccgttgg gtatatgaag ggtttgatgc tacctataaa acacttggca
     5401 tcgaattcga taaaacctat tacgaatcgc agacatatct tcttggtaaa tcactggtaa
     5461 atgaaggcgt cgttaaaggc gtattcgcaa agcgggatga taattcggtg tggattgacc
     5521 tgaccaacga cgggcttgat gagaaactgc tgttgcgaag cgacgggact tcggtctata
     5581 tgacgcagga tatgggaacc gcccagcagc gttttgagga atataaacct tcccaactca
     5641 tttacgtggt cgggaatgaa caggagtatc atttcaatgt gctgaagctg gtgctgaaac
     5701 acctgggctg tgaatgggcc gatataatat accacctttc gtacggtatg gtggaacttc
     5761 cgcatggtaa aatgaaatcg cgcgaaggga cggtggtaga tgccgacgac ctgatggaag
     5821 agatgttcga aacggcggaa cggatggccc gggaacttgg caaactggaa tccttcgaag
     5881 gcgaagaagc tgaaaaactt taccggacga tcgggctggg tgccttgaaa tatttcatcc
     5941 tgaaagtcga cccgaaaaag aacatgcttt ttaatcctga agaatcggtc gattttaatg
     6001 gaaacacagg gcctttcgta caatatactt acgcccggat tcaatcggta ttgcgtaatg
     6061 ccgtcaagct gggcattgac ccggaaagcc ggggcagttt cgaaatgagt gcggaaattt
     6121 tgccgcagga aaaggaagca ctgaggcttt tgcacgattt tccggcaacg gtttcacagg
     6181 ctgccgacat gcacagccct gccgttattg ccaattatgt gtatgaactg gccggtgcat
     6241 ataatcgttt ttaccacgat attaccattt taaaagaaga gaacgcgccg gcccgcaatt
     6301 tccgccttgg attatgcaac ttaacaggca atgttatcag gaatgcgatg tggatgctcg
     6361 gaattgacgt tccggagagg atgtagcaga cgagggagcg acaatgcagc tatacgggag
     6421 aaagactttg ctcagggaga gcgatagttc tttccggtga aataaactgt gatattcctg
     6481 aaatccgggt ttccggcatt tcatcttaaa cataatcttc ccccggcgct gttttaaggc
     6541 gccatcagga ttttttagag acttacgaaa gagcaattcc ttgtcattga cggtcaagga
     6601 caaagtcgtc ttcgtaaacc tcgatctgaa ggtcggagcc aatcagttca acgagccatt
     6661 ttttgcattc gcgcctgatc agcagccgga tccttataaa tcccagtaca ggatatttta
     6721 aagtagcaaa catgtttttt tatggcagct ggttcttttg aatataagcc ggattaccat
     6781 ttcctttacc tgtttatcag gcttttataa aaaggtaatt tccttttact gaatttttgg
     6841 aaagattttc cgggataatc tgccggaaag caaaacacgg ctgcagggaa atgtgaaaac
     6901 tggtttaatg tattgtaatt caagcgttat tatatagagc ctgataatga agagaatcca
     6961 tctgccataa aaccctgttc aaatttgccg ccttgtgcca ttgaaaatga tcctcttaaa
     7021 aatgctgtac ttctaatcta acaggtaagt tttccggtta ccggagccat cataactgct
     7081 gaaacagatt ttgttttggg cggagaggtt gaatgagaat aaaaagagta tcattccaaa
     7141 gtagcgttta ttcatcttaa tttgatttat ttttgatgta gtatgaatta ttattatatc
     7201 cttcagtctg aattttgaga atagaaaaaa tgactcatgg tctcaaatat tgaaatcgct
     7261 tcttctttcc tatcagtctt ttaaatcggc tatttaaatt ttcaggccta caaataaatt
     7321 tttttcctgc cgggtcatag tttcgtttta ttcgatggac agccggacaa ctaatctcgg
     7381 ggtaataaat gacataatag attttgtaaa aattacgact cttacttatt tcaaaaagtg
     7441 tcagaaacgg agaataaaca atataaacac aacccgtctt tgaatcactc aaaaacgccc
     7501 ttgaaattga atctgtataa gatttatttg atgaaccaag aagtttcagg ttaacaccac
     7561 aaaacaaact atcgttgtct ttatattgta agtaataaat tttgtcatga tagaaataag
     7621 ctgaattgta atttcctatt acttcatcat aatacactac tgaagagatt gaaacattaa
     7681 ttctttgatt atcaatattt gagaatttat tgatatatcc acaggggtca ttgctttggt
     7741 gatcataact tatttcgtta agatacgaat atttgtctat cattgtattt agtagtctca
     7801 aaccgtcacc agactgacag agacaaatat ttgagattaa aacgaggatg atcagaatta
     7861 cttttttcat agaatggatt ctatcgtcgc tgtcttgaat aatgtctaga gttgtaatta
     7921 tttaaataat ctgatttatc aaacattgtt gtattcaatg gtacaaatga ttgtcggaat
     7981 gctaaattat tattcatagg tatccacttc aaaagattag tgaagagtat cgttaatcct
     8041 aaacatcatg aattgctcgg gtagggtagc cacatcaata tatgtttcct ggcttaattc
     8101 atgtgcctgg tttttactta tcagtattat cctcggtata ataacaccac cggcgataat
     8161 gttttccgga agcagatggc tttcaataat tctccttttt gtcctttgaa tagcggttag
     8221 ttttttaaaa tcacttttac tcatgatttt ttttgcatca caattaatga aattgaaaac
     8281 aatgcattta ttattaaact caaaataata gtcagcttta tttacatcaa gactgagatg
     8341 gttatagata gctgctaaag aaatagcaaa agtgtcattc ttaaagtcat acagtgatac
     8401 atataatact tttctacaca ttagatctgc gttgttatcc acctgaagac tgagatcctg
     8461 gcaatagagt tctgctttgt tgctgatata tgaaagcggc agcagtgatt gtccatatgt
     8521 caatactgat gatatctgaa ttgcgaaaac aatgatcagt cttttcatgc tttgaggtat
     8581 tagatttatc tttccctgat cctgaaagag ccttgattac gattaagaaa attcaggtag
     8641 tcagcatttg gtaaaccatt cccatccaaa aattctaaaa tgggtatcct ggtttctttg
     8701 ccatttcccc gtactatcca gtatttggtt ggtgatgttt ctatcggatg atatacttta
     8761 tagttagcct ttctgtggtt attgatttta tatagaaaca aaaaggggaa atgtaagacc
     8821 ccatcgttta ttgaattctg attaaatgaa gcatctgtaa tgctcttgat cgtttgagga
     8881 tcaataatgt caccggtgtt ttttcgaaca atatttatgt tgctatcatt ttcaaaataa
     8941 aacactctgt ttccgttttt ataacatttg acattatgca atggcacttt atctgccatg
     9001 ttcatatctg caaatgcgac cgagtaagcg ttggtctttt cttctttctg aatcttaaag
     9061 aaaaatgtat ttaagtttga aagaataggc tctttgtcaa gaatgaaatt aagatgacta
     9121 attattatat ctatctcagt ttcgtttata gaaataggtt gggagtatac tttagactga
     9181 aataagacca taagtaatag gaagactctt ttcatttttt ttatttcagt tttagtttaa
     9241 atccgaaaat taaatctgcc aatatttata attttcttcc agattcagtg aaagacctat
     9301 tgttccaatg aatgcataat ccggccggat ggacgtcgac aggacaaaga tagttagaag
     9361 aatttactat tcattctcct ggttacaatt gatcaatacc ggcggttaga gtgcctgata
     9421 atgatgaagg gccgcctgcc ataaaaccct gttcaaattt gccgccttgt gccatagaaa
     9481 atgatcttct taaaatgctg taatctaatg taacaggtta gtttttcggt tgtatgagcc
     9541 atcataactg ctgaaacaga tcttgttttg ggcggagtgt aaaataggta acggtgacaa
     9601 tgctatcaat ataagatgtt ctattttcat aaactttctt tgttttctat gttgttatat
     9661 atattgttat cactttctgt tattatcggt tttaggaaca gtaattttaa accttccttt
     9721 tttaattaaa tctcgatatt catcatttgg gttgttttgt ttgtctagaa aaccaccgta
     9781 aatactctgt gtatctgaat acaaacgtgg tgaaataggc cagtattttt tcggaacctg
     9841 atcaattgga tgaaatgtat tataggttac ttttcgatgg tttaaaatcg gtatcttctt
     9901 atgtacttta aataatgtta aaaccggaaa tgaaataatt gccccttctc ctctgaataa
     9961 tttatataag aacagccttt tctcatcggc tgatagatta aataatagac tatcatgttt
    10021 tgaatgtttt aattcaattc tatcatcttc ttgaagaaag aagtatgtat tatctattaa
    10081 ataataataa tatgtatctg ctgaaagagc ttcatcccga tttatcccgg ttatggaaac
    10141 actgtatgtg ttcatattgt cttccgtaat tactttgata gcatttatac gaccaatgct
    10201 ccatcgggaa aattttggca tgcccaaaaa ttttgagaag atttgttgca gttcaaaact
    10261 ttcaacaatc actttttcct gactcaaact aatatttgag tacaagacta aaaaaataac
    10321 tattattttg aatcttttca tggtttattt gctccgtatt ttgcattaaa gtaatcagta
    10381 cgatatttag gattgcaaaa atcagctcta ttttcaggaa agaaaaatga agtcaaaaac
    10441 actgggccaa ctatagtcca acttcctaat cctattgtat ggtgtgtaac agaaacttgg
    10501 taatcttggg cagctataaa atcattttta ctaacagtat aataagaagg tgaactacaa
    10561 tcactacaat cgcggattga ataatgatct tcttcaacca atgtatatcc ctcagatttt
    10621 aggcatctaa ggtttgtagg agtggcattg tcctcaccat tattaataaa tgcttctaaa
    10681 aaataacttt tattaccatc actatctatt aaggaaaata tctgtgtttc ctttttgatc
    10741 atagatgtaa tattcaccgc tttctttaca gcttctgctt tggaattata cattctgcca
    10801 taattttcta tctgtttata ttcttccaaa tttttaatcg aaagtttcag aaaagcatca
    10861 tgcattaaat gattatacat cactgtaaag gcccccgtta cagccccatt ggcaaatttg
    10921 ccgcccccga tttctgcggc tgtgccgcct gcaacagtgc caatgacaac ttcggctgca
    10981 taattatcaa ttcctgcggt cgcagagcct gataatgacg agaatccgcc tgccataaaa
    11041 ccctgttcaa atttgccgcc ttgcgccatt gagaataatc ctgcttaaaa gtatttgtac
    11101 ttatatcacc atatgcatgg tttccggtta ccggatccat tataactgct gaaacagatc
    11161 ttgttttggg cagaaatggt cacagagact aaaaatatag taataaattc aaaatgcatc
    11221 tttaggtttc tcatacttga gagatgtatt agattattat gttaaaatta ttttagccgc
    11281 aatggctttt gtttattaat tttttttaaa aactctttat taatcttatc tttccaggga
    11341 taatattttg tgcctgcagg ataatataat cgatttattc tttctttttc aggacagttt
    11401 ttttcaggga aatagattat agaggatcct ttgatttttg aatttacttt ttttatataa
    11461 taggcagcta tgaaagggta gtaaattgta tattctcctt tctgtgaatc ttctaaatat
    11521 aatcttgaga tgtatttaat gctgtcagta atagatgttc caagaagatt caaattaaaa
    11581 cccaaaaaaa tactgtcatt tttattgtat tgcaagaaat atctttgatt ttgaaattga
    11641 tatatggaat tacagttccc aagcacttcg tcaaaatagt atattgtaga acactgttgt
    11701 ccggtaaaaa aatataaccg ggtcagccat tttgcagact aacc
//
LOCUS       DAOPYB010000092        11624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6316_length_11624_cov_10.802403, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000092 DAOPYB010000000
VERSION     DAOPYB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6316_length_11624_cov_10.802403"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6313)
                     /locus_tag="PKH58_08095"
     CDS             complement(<1..6313)
                     /locus_tag="PKH58_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89026.1"
                     /translation="MKKLLLLVSFVVCTTLSFAQGNMISMTSQLPTEITICGPEQKVT
                     LNIVNPSPFGLHDVVLQLTMPDGINYVPGSVTGAVELDTSNLSTPQFTLANISTQGSR
                     NISFYISANCDVMDFIAQGQTVSVVSRVDYITSNNIDTYDINTSYLYHVKQPNLSIIS
                     ITNQTYSGNIGDVFTRCITITNGGLGELSNFTFTHTHGNGINVTNVSIGIWNSAGNTE
                     TVYFDGNDFTGTGNHNNLFENAEVITICETIEVLNCVSVFSDYEVKWGCEGAVCQTSI
                     ATANVVFPNLTPNLNVVVLTTGVNTCLGFGHANTPALRIINNGMGDAYDVFLDIFHAT
                     STIYYNSGLISNIDPGSFTMQVNSNAPVSITPTQTFNNSSFYCLSPNPKGRVYLNIPV
                     INSHDTVIIRWVYYNCCANQCGGTRVISGWRYQGSYKSICDNNYVIPNAWGYSYKEIY
                     GDLVNDLSPSNITSGETKNFSFLFSSYGVSYPSTTGRYWKFVVLLPRCLNYAGNFLIQ
                     SYTGTQFWYPTTVNVSGDTLTAIFNNWPPFTLFQSVVKFDLSANCANAGCNEGGNTLS
                     IKSYFIPDGSCGCEVNVSCLSITVGVICPVTCEGLNNKGFETYRSSYGSPDNDNNGLA
                     DPLPATLNFGLIRRDRAMYNDTITTLSKSTVKTSLAHPNWNYFYRIDSILNGNRLEFI
                     DGYLEIFQHSTGNVYTCNVNPVLPITTVGTTRKFKYDVSPVTLSALGYLPAGFTLNDG
                     DSIAFKVRYRVNSNTGGAILDCFVKNKMFVSDVPNPTSFAHKFGCNVFEGRFSIIGFY
                     YTNWGPDNFSVNTCNTFTISQNYYLSIGPCCQNYAGGNLFPFEYRNWAHPLRFRVVLP
                     HGYQFISAVFNQVRTAGTTLTSNSGNIGLSPVNPNSDTLVFWVESFFQTFGGSVPLSD
                     DGFYGTLSVTLAPTCNVVSNVTSGIGYLWDFAPSGSLSGYTANTSYVTTHDYITYQGP
                     DIFLQSSLPSILAYNDEVSWDISLSNTTNYNAMNTWFSGDQTTGVTILQVWDLDNNVL
                     VSPVGNIYQIGTLPGNVTRNFRIIATYSSCFPSTMMVYAGWNCNAGYPATVASYPCTA
                     EQILLSLEPQVPNLIANVSSPFSTVDLCDTAGYVVEGINVQLGTAYNLKLKVVLPAGV
                     SIIPGSSKLSYPAPGAFVNIPDPTWIAGTQWQWDLSAINALIGTDGLPGILDTAHNSV
                     KISFKVMTDCNYTSGSVIGFNFHGQSHCGNYTGQEITMSSQLAITGATEPYLTDIHVN
                     TSYVSPCAFNSTRLKIKVVNNGPLPTGTTDSISLTFPEGIFYIPNSFVPVDNAPVNPV
                     PVTEMPNNMQIVTWRLPQNTQAGDSVVFEIYYYCVADVVECGIYYIDAMTYSSRNLLC
                     QLTGQNCDILVSTGSAQIPIYIYKSYLSLSDASAWSVPNGSNQETVYTGYTISNNGES
                     IISNHTVYLSYFNDADGNQMLSSPDNFLGSDAINVFIDTNSVNPFTSQINVPAGNSCK
                     LLFVLDTTANGCICDLNQIYANVPLLNAGPDTVFCAGQSAYIGMPPTNGYTYQWQPST
                     GLDNPNSSRPLFTGSNTGTTPVTLTYILTTNRITCTTRDTVVIVVRPIPVVNANIDQD
                     IGVCPSAANAPLNGSATGSAPLVYSWTATTGLSNPNIPDPVADPLVTTTYTLTVTDTY
                     GCSASDDVMINVHPLPTAVVSIDQNIGACPTAASANLVCIATGTPPVTFSWLPATGLS
                     NPNDANTIADPFSTTIYTVTVADTFGCTATDVVIVTVDPLPAVNAGTDQYIGSCPNAA
                     NANLNAVATGTAPLTYSWLPIAGLSNPGITNPLADPASTTTYTLTVTDTYGCTATDAV
                     LITVDPLPTVNAGNDQNLGACPNAANANLNAVATGTTPLTYSWLPAAGLSNPNIANPV
                     ANPANTTTYTVTVSDTYGCTVTDAVLITVDPLPTSNAGTDQHIGACINAANANLAGTG
                     TGTGPLVYSWLPTAGLSNPNIANPVADPASTTTYTLTVTDTYGCTATDAVVITVDPIP
                     TCNAGTDQHIGACINAANANLSGTGTGTGPLVHTWLPTAGLSNPNIANPVADPASTTT
                     YTLTVTDTYGCTATDAVLITVDPLPTVNAG"
     gene            complement(6613..7845)
                     /locus_tag="PKH58_08100"
     CDS             complement(6613..7845)
                     /locus_tag="PKH58_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX89027.1"
                     /translation="MAQTLFIIILIIVVFNYLFGLLLDYLDSTLWSNVLPKELNGIYD
                     AEKYRKSQDYEKVNTRFSLITGAFSFILLILMLVLGGFAWLDNFVRSYASDPIIIALL
                     FFAVLGLTSDLLTTPFSAYHTFVIEQKFGFNTTTVKTFILDKLKSWLLAAVLGGGILT
                     FIVWVYGNTGSWFWFVAWMALTGFSIFMTLFYSNIIVPLFNKQKPLEAGDLRDAIEAF
                     AIKTGFKLKNIFVINGSKRSKKANAYFTGFGPKKRIVLYDTLLNDFTNDEIVSILAHE
                     TGHYKKKHVLYGLLTSIISSGVLLYIMSLVIGSPDVAGALGAGEASFHMGILAFGMLY
                     SPVSMILGIAGNYISRRHEYAADHYAAIQSNTDAMISGLKKLHVLSLSNLRPHPFTVF
                     ITYSHPTLLQRIAALNKS"
     gene            complement(7958..8722)
                     /locus_tag="PKH58_08105"
     CDS             complement(7958..8722)
                     /locus_tag="PKH58_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNX89028.1"
                     /translation="MFFIISKITAFLLAPLTWIIILLVTGLFVRNAKRKKRIYITAVV
                     FSIFFTNPVIIDELLRCWEIPYTAVEKNSVYEAGIILGGGMVTIDQINDKLIFQENTD
                     RFLQGLHLYKNGQIKKIILSGGAGNLVFKNFLEASLIKRYLVEIGVPEADILVDSASD
                     NTYQNAVNCKEIIKNNFSDGRYLLITSSLHMKRAWACFSKQGITVSAYPTNKFVGLRR
                     TDVGYYLIPSTGALSRWEKFIHEVLGYVIYAIMGYL"
     gene            complement(8754..11363)
                     /locus_tag="PKH58_08110"
     CDS             complement(8754..11363)
                     /locus_tag="PKH58_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89029.1"
                     /translation="MIYQNIEEMALKSSYTTAPDQNQKYTNFFISEIARSIPKKTEYT
                     QYFFNCRKIDRINETEPGKYSATFEIMDAGCNGDVFYKGFSIADVLIPTNCSFTFSVF
                     SDKAIPLYQTTVSDGILSPGYNILASYKFSDSSALKKFNTRVENFAFHYDENSISRFN
                     SKLKLIDEYFLSGTTIDQCLAKIKEIDYGNTDMIIVYDISLKELEKNSEELYNKGFPG
                     KLNLVDYDPVQFIDKYNKLSETLFNTRNQLNLKLENLDKLYYEKGLAEVEKAETAKAK
                     MYFNRSVLYNPDFVPSQLEVAKILYHCDSLNAAADKISQILRTLKPTPDIYHQVILYT
                     DSVYNRMLATGNEYNKLEKYNEAIEVLEKCVSFCDGLPGYSCGEKHVKSLAAARFGIY
                     QSYLSVSQKAIDNGKYELAEIYISDARNYQKSYSSFIINDAEAVTKLDKIMAAYVAMG
                     DTLISEKKYEKGLLYLEKAKNISEKNKLALPENFEKSISKARTGIYTSMLKKCSHQIG
                     LRLIEPAEKTLNEAVDYQNKYSEIIQPSKGVDSLFVKIRYYQYEDLINSGLINQNMGN
                     NQTALQYYDQAKKLEQEYHFKQNPMLDSLIQTSAKPMIETQIILIEKLITAAKTDSAI
                     SLNDRISKSMAIHGLSNDSGLMAKARLLKKNIYDLRCGQAKNIFDDRMKQANMAVARQ
                     HFMESDSLYSQAITVSDSFADCRIDANSAIDGKKTYLLPCNYQKMILKASTALNHDDY
                     NTFLYNYSEAENFYINIQLNNYGLVHQKLTDLVAVSSDTSLIFAATDFMIQQNRPEDA
                     LRCLKTLQKMNYDALMTKSMQLQSGAMMARKDHAVNASSQPSVLVVSYAGNDKWLSYF
                     RASYIKTWKSLNK"
BASE COUNT         3484 a         2318 c         2436 g         3386 t
ORIGIN      
        1 taccagcatt gactgttggt aaaggatcaa cagttatcag aactgcatcg gtagcagtac
       61 aaccgtatgt atcagtaacc gtaagtgtat atgttgtcgt ggaagctgga tcagcaacag
      121 gattggcaat attaggatta cttaatccgg cagtgggcaa ccatgtatga acaagcgggc
      181 ctgtccctgt tcccgtgcca gataagttag catttgcagc attgatacag gccccgatat
      241 gctgatcggt accggcattg catgttggta tgggatcaac agtaatgacc acagcatctg
      301 tagcggtaca gccataagta tcggtaacag tcagggtata agttgtcgtg gaagctggat
      361 ctgcaacggg attggcaata ttgggattac ttagcccggc agttggcagc cacgaatata
      421 caagcggccc ggtgccggtt cccgtacctg ctaagtttgc atttgcagca ttgatgcagg
      481 caccgatatg ctgatcggta ccggcattgg atgttggtaa gggatcaaca gttatcaaaa
      541 ctgcatcggt aactgtacag ccataagtat cagaaacggt aacagtgtat gttgttgtat
      601 ttgcaggatt ggcaacagga tttgcaatat taggattgct taatccagcg gctggcaacc
      661 aggaatatgt caacggcgtt gtaccagttg caacagcatt taaatttgcg ttagcagcat
      721 taggacaggc tccgaggttc tgatcattac cagcattgac tgttggtaaa ggatcaacag
      781 ttatcagaac tgcatcggta gcagtacaac cgtatgtatc agtaaccgta agtgtatatg
      841 ttgtcgtgga agctggatca gcaagaggat ttgtaatacc aggattgctt aatcctgcta
      901 taggcagcca tgaatatgtt aaaggagctg ttcctgttgc aacagcattt aaattagcgt
      961 tagcagcatt aggacatgat cctatgtact gatctgtccc ggcattgacc gcaggtaaag
     1021 gatcaacagt tacaataaca acatccgttg cagtgcaacc aaatgtatct gcaacggtta
     1081 ctgtataaat agtcgtagaa aatggatcgg ctatcgtgtt ggcatcgttt ggattactca
     1141 ggccggttgc gggcagccat gaaaaagtaa caggtggggt tcctgtagcg atacatacaa
     1201 ggttagcact tgctgctgtt gggcaagcac cgatgttctg gtcaatactt actactgccg
     1261 taggcaatgg atgtacattg atcattacat catcggaagc cgaacacccg tatgtatccg
     1321 taaccgtaag ggtataggtc gtagttacca acggatctgc aacaggatca gggatatttg
     1381 gattactaag cccggtagtt gctgtccatg agtaaaccag gggcgccgaa cctgttgcac
     1441 tgccattcag gggtgcgttt gccgctgacg gacagacgcc tatatcctga tcaatattgg
     1501 cattaacaac aggaatcggc cggacaacga tgaccacagt atctctggtt gtacaggtta
     1561 tcctgtttgt agtaagtatg tatgtcagtg ttaccggcgt tgttccggtg ttggaaccgg
     1621 tgaacagcgg ccgtgatgaa ttgggattat ctaagccggt ggatggttgc cactggtagg
     1681 tatatccgtt agtaggcggc atcccgatat aagcagattg tccggcacaa aaaacagtat
     1741 cggggccggc atttaaaagc ggcacattag catatatctg attgaggtca catatacatc
     1801 cgttagccgt ggtgtcaagg acaaaaagaa gtttacagga attgcctgca ggtacattga
     1861 tctgcgatgt aaaaggattg actgaattag tgtcaataaa aacgttaata gcatcagatc
     1921 ctaagaaatt atccggtgaa gaaagcattt ggttcccgtc ggcatcatta aaatagctta
     1981 gatataccgt atggttggaa ataatagatt ctccgttatt ggaaatggta taacccgtat
     2041 agacagtttc ctgattcgag ccattaggca ctgaccaggc agaagcatct gataatgaaa
     2101 gataagattt ataaatgtaa atcggtatct gcgcactgcc ggtactgacc aggatatcac
     2161 aattctgccc ggtgagttgg cataataagt tccgtgagct ataagtcatg gcatctatgt
     2221 aataaatacc gcattcaaca acatccgcaa cgcagtaata atatatttca aaaacaacac
     2281 tatctcctgc ttgggtattt tgaggaagcc gccaggttac aatttgcata ttattgggca
     2341 tttcagtaac aggaacaggg tttacaggag cattatccac agggacaaaa gaattgggaa
     2401 tataaaaaat cccttcagga aatgtcagtg agatagaatc tgttgttccg gtaggtagtg
     2461 ggccgttatt aacgactttt atctttaatc tcgtgctgtt aaaggcacat ggtgagacat
     2521 aagaagtatt gacatggatg tcggtcaggt atggttctgt ggccccggta atcgcaagct
     2581 gggacgacat tgttatttcc tgtcccgtat agttgccaca atggctttgt ccgtgaaaat
     2641 taaacccgat aacgcttcca gaggtataat tacaatcggt catgaccttg aatgatatct
     2701 taacggaatt atgagctgta tcaagtattc cgggtaatcc gtcggtgcct atcaaagcat
     2761 tgatagcaga aagatcccac tgccattgcg tgcctgctat ccatgtagga tcgggaatgt
     2821 taacaaaggc gccgggcgcc ggataactca gtttactgga acccggtatt atcgatactc
     2881 ctgccggcaa aaccaccttt agcttgagat tatatgccgt acccaactga acattgatac
     2941 cttcaacaac ataccctgct gtgtcgcaaa gatcgaccgt agaaaacgga ctagaaacat
     3001 tggcaatgag attgggcacc tgtggttcaa gagaaagcaa tatctgttcc gcagtacacg
     3061 ggtatgatgc aacagtggcc gggtatcctg cattgcagtt ccagcctgca taaaccatca
     3121 ttgtgctggg gaaacacgaa gagtacgtag ctatgatcct gaaatttctc gtgacattgc
     3181 ccggcaaagt acctatttga taaatatttc caacgggaga aaccaataca ttgttgtcaa
     3241 gatcccacac ctgcaaaatc gtgacgcccg tcgtttggtc gcccgaaaac caggtgttca
     3301 ttgcattgta attggttgta tttgacaaag aaatatccca actgacctcg tcgttgtatg
     3361 ccagaatgga tggcagggaa gactgcagaa aaatgtcagg cccctgatat gttatataat
     3421 catgggtagt aacataagat gtatttgctg tatatcccga cagacttcct gaaggtgcaa
     3481 aatcccataa gtatccgata ccggaagtaa cgtttgaaac aacattacat gttggcgcca
     3541 gcgtgactga caatgtgcca taaaatccat catcgctcaa aggtacggat cctccgaaag
     3601 tctgaaagaa agactctacc caaaacacca gcgtgtctga atttggattg acaggtgaca
     3661 ggcctatgtt tcctgaattg gatgtcaacg tagttccggc agtacgcacc tgattaaaaa
     3721 cggctgaaat gaactggtac ccatgtggca ggacaaccct gaacctgagc ggatgagccc
     3781 agttgcgata ctcgaaagga aacaaatttc cccctgcata gttttggcaa cacgggccaa
     3841 tactcaggta gtaattctgg gatatcgtaa acgtattgca tgtattgaca ctaaaattat
     3901 cagggcccca gttggtataa taaaatccaa tgatagaaaa acggccttca aaaacattgc
     3961 agccgaattt atgggcaaat gacgttgggt tgggaacatc actgacaaac attttgtttt
     4021 tgacaaaaca gtcaagaata gcccctcccg tgttactatt gacccggtat cttaccttga
     4081 aagcgataga atcaccatca ttaagggtga atccggcagg caagtaaccc aatgcagata
     4141 aggtgacagg gctcacatcg tatttgaatt ttcgggtagt gccgacggta gtgatgggta
     4201 atacaggatt tacattacag gtgtatacat tacccgttga atgctgaaaa atttcaagat
     4261 agccgtcaat aaattcaagc cggtttccgt tcagaatcga atcgatcctg taaaaataat
     4321 tccagttggg gtgggcaagg cttgttttaa cggtactttt agataaggtg gtaatggtgt
     4381 cgttgtacat ggccctgtcc cttcttatta aaccaaagtt cagtgtggcg ggaagcggat
     4441 ctgcaaggcc attattatcg ttatcgggcg atccgtagct gcttctgtat gtctcaaatc
     4501 ccttattgtt tagtccctcg caggtaaccg gacagataac tccgacagta atactcaggc
     4561 atgaaacatt cacctcacaa ccacaactgc cgtcagggat aaaatatgac ttgatcgata
     4621 aggtatttcc tccttcatta cagcctgcat tggcgcaatt tgccgacagg tcaaatttta
     4681 caacagactg gaataaggta aatggcggcc agttattaaa tattgcggtc agcgtgtctc
     4741 ccgaaacatt cacggtggta gggtaccaga attgagtccc ggtataactc tggatcagaa
     4801 aatttccggc atagtttaaa caacggggca aaagaacgac aaatttccag tacctgcctg
     4861 ttgtactcgg ataactaaca ccataactgg aaaagagaaa actgaaattt tttgtttctc
     4921 cactggtaat atttgaaggg gacaggtcgt tgaccagatc gccatatatc tctttgtatg
     4981 aatagcccca ggcattgggg atcacataat tattatcgca tatgctttta tatgaaccct
     5041 gataacgcca ccccgaaata actctggtgc cgccacattg attcgcgcag caattataat
     5101 atacccacct gatgatcacg gtgtcgtggg aattgattac agggatattg agataaaccc
     5161 tgcctttagg gtttgggctt aaacaataaa atgaggaatt attaaatgtt tgcgtcgggg
     5221 taatactgac aggtgcatta gagttgacct gcatggtgaa gctgccggga tctatgttag
     5281 aaattaatcc tgaattatag taaattgttg atgttgcatg aaaaatatcc agaaaaacat
     5341 cataggcatc ccccatccca ttgttaatga tcctcagggc cggagtgttc gcatgaccaa
     5401 atcccaaaca ggtatttaca ccggtagtaa gtactacaac attcagattg ggtgtcaaat
     5461 tcgggaaaac aacatttgct gttgcaatgg atgtttggca aaccgcaccc tcacatcccc
     5521 attttacttc ataatcagaa aaaacagaca cgcaattcaa tacttcaatg gtctcgcata
     5581 tcgttattac ttcagcgttt tcaaaaaggt tattatgatt gccagtcccg gtaaaatcat
     5641 ttccatcgaa ataaaccgtt tcggtattgc ccgctgaatt ccagataccg atactaacat
     5701 tagtcacgtt tataccatta ccgtgcgtgt gagtaaacgt aaaattactt aattcaccca
     5761 gtcctccatt tgtaattgtt atacatcggg tgaacacatc tccgatattt ccggaatatg
     5821 tctgatttgt gattgaaata atagacaaat tgggctgttt gacatgatat aaataacttg
     5881 tattaatatc ataagtgtct atgttattac ttgtaatata gtcgactctg gatacaacag
     5941 atactgtctg gccttgcgcg ataaagtcca tgacatcaca atttgctgaa atgtaaaatg
     6001 aaatatttct tgacccttgt gtagaaatat ttgccaaagt aaattgcgga gtactcaaat
     6061 tgcttgtatc taactcgaca gcgccagtaa cagagcctgg cacataattg attccatcag
     6121 gcatcgtcag ttgtaaaaca acatcgtgaa gaccaaaagg agacgggttt acaatattta
     6181 atgttacttt ttgttccggg ccacagatgg ttatttcagt gggtaactgc gatgtcatac
     6241 taatcatgtt tccctgagca aaagataatg ttgtgcatac aacaaaagaa actaataata
     6301 aaagtttttt catgtattat atttctaaca agtattaacc attaataata tcgtgtctat
     6361 gaccaagcat tttctatttc aaccaataaa ttttttttat gatcttttca acagatgata
     6421 atagatagta cgtttttctt atctcgaaca ccaacaaaga tacaataata cagttagaca
     6481 tcaaattatt taacgaaaaa atcaaggtat aatccaggcc tcttttttga aataaattaa
     6541 ttttatcgca tgggttttct ttaccaggaa gttttatttc atgttatatc tttcagaaaa
     6601 ccgaacaaaa aatcaagatt tatttagtgc cgcgatcctt tggagcaggg ttggatgtga
     6661 ataagtaata aatacagtaa aaggatgagg acgtaaattg ctaagactta gtacatgcaa
     6721 ttttttcagt ccgctaatca tcgcatcagt attggattgg atggcagcat aatggtcggc
     6781 ggcatactcg tgccttcgtg aaatataatt accggcaatg cccagtatca tggaaaccgg
     6841 actgtacaac atgccgaaag caagtatgcc catgtggaaa ctggcttcac cggcgcccaa
     6901 agcacctgcc acatcagggc ttccgatcac caatgacatg atatacagca ggactccgct
     6961 actgataata gaagttaata gaccatacag cacatgtttt ttcttgtaat gtcctgtctc
     7021 gtgggcaagt atagaaacga tctcatcgtt agtaaaatcg ttcagcaggg tatcgtagag
     7081 tacgatcctt tttttcggcc cgaaaccggt gaaataggca tttgcttttt tcgaacgttt
     7141 tgaaccgttg atcacaaaga tatttttcag cttaaaacca gtttttattg caaaagcttc
     7201 aatagcatcg cgtaggtctc ctgcttcaag tggtttctgc ttattgaaaa gaggaactat
     7261 aatattcgag tagaataatg tcataaaaat tgaaaatcct gtcaatgcca tccatgcaac
     7321 gaaccagaac catgatcccg tattaccgta tacccaaaca ataaaagtca atatcccacc
     7381 gccaaggaca gcagccagca gccacgactt aagcttgtcg aggataaatg tttttaccgt
     7441 agtggtattg aacccgaatt tttgttcgat cacaaatgta tgataagcgc tgaagggggt
     7501 tgtgagcagg tcggatgtca gtcccagcac agcaaagaaa agcaaggcta tgattatggg
     7561 gtcggaagca taagatctta cgaaattgtc gagccaggca aaaccgccca gaacaagcat
     7621 caggatgagc aggataaaac tgaatgcacc ggtaatcaat gaaaaccgtg tatttacttt
     7681 ctcgtaatcc tgcgatttac ggtacttttc ggcatcgtat ataccgttaa gctccttcgg
     7741 caatacattg ctccagaggg tactgtccag gtaatcgagc aataaaccaa acagatagtt
     7801 aaaaacaaca atgatgagaa tgatgataaa aagtgtttgt gccattgctt atgaattgat
     7861 tgacaaaatt agttattaat aattatcgga ttgtttgctc aaatcatcca tgcaaccggt
     7921 ttttcatccg ggctaaggat atgcagggct ctgtggatca taagtacccc attatcgcgt
     7981 aaataacgta gccaagaact tcgtggatga atttctccca gcgcgacagt gccccggtgc
     8041 tgggtatcag gtaataacca acatcggtcc ggcgcagccc cacaaattta ttggtaggat
     8101 atgcgcttac agtaatccct tgtttagaaa agcatgccca ggcgcgcttc atgtgcaaag
     8161 atgatgtaat aagcaaataa cgtccatcac tgaaattatt cttaattatt tccttgcaat
     8221 ttacggcatt ctggtaggta ttatctgacg cggaatcgac aaggatgtcc gcctcaggga
     8281 cacctatttc gacaagatag cgcttgatga gtgaagcttc cagaaaattt ttaaaaacaa
     8341 ggttcccggc ccctcctgat aatattatct tttttatttg cccgttttta tacagatgca
     8401 gtccctgtaa aaacctgtca gtgttttcct gaaaaataag tttgtcattt atctgatcga
     8461 tggttaccat gcctccgccc agaataattc cggcttcata gaccgaattt ttttctactg
     8521 ctgtataagg gatttcccaa cagcgcagca actcatcaat aataacggga tttgtaaaaa
     8581 aaatcgaaaa tacgacagca gtgatataaa ttcttttttt acgtttcgca ttcctgacaa
     8641 ataatccagt aaccagcagg ataataatcc atgtcagcgg agccagcaaa aaagcagtta
     8701 ttttcgaaat aataaaaaac ataaatgcaa aataaagaaa atttcaaata ggactattta
     8761 tttaaagatt tccatgtttt aatatacgaa gctctgaaat aactcagcca tttatcattt
     8821 cctgcataac ttaccactaa aactgacggt tgggaagagg cattcaccgc atggtctttt
     8881 cgcgccatca tggcaccgga ctgtaactgc atacttttcg tcatgagggc atcatagttc
     8941 attttttgta atgttttcag gcatcgcaag gcatcctcgg gacggttttg ttgaatcatg
     9001 aaatctgtgg ctgcaaaaat taaggatgtg tcagaagaaa cggccactaa atctgtcagc
     9061 ttttgatgca ctaacccgta attgtttaac tgaatgttta tataaaaatt ctccgcttcg
     9121 gaataattgt acaaaaaagt attatagtca tcatggttca aagctgtact tgccttcagg
     9181 atcattttct gataattgca aggcagcagg tatgtctttt ttccatcaat ggctgagttt
     9241 gcgtcaatcc ggcaatcagc aaagctgtca cttaccgtga tcgcttgtga gtataatgag
     9301 tccgattcca tgaagtgctg ccttgcaacg gccatatttg cctgcttcat acgatcatca
     9361 aaaatatttt ttgcctgtcc gcaacgcaga tcataaatat tttttttcag cagcctggct
     9421 ttggccatta gtccggaatc attgcttaat ccatgaatag ccatgctttt agaaatcctg
     9481 tcattcaaag aaatagccga gtccgttttg gctgcagtaa tcagcttttc gataagtatg
     9541 atctgggttt caatcattgg ttttgcgctg gtttgaatga gcgaatcgag catggggttt
     9601 tgcttgaaat gatattcctg ttccagtttt tttgcctgat cataatactg caaagccgtt
     9661 tgattgttac ccatgttttg gtttatcaga ccactgttta taaggtcttc gtattgataa
     9721 tacctgattt ttacgaacaa agaatccacc ccttttgaag gctggataat ttctgaatat
     9781 ttattttgat aatcaacagc ttcattcaac gttttctcgg caggttcgat aagcctcaaa
     9841 ccaatctgat gtgaacactt tttcagcatg gaagtatata ttcctgtccg tgcttttgaa
     9901 atactttttt caaaattttc agggagtgcc agtttgtttt tttcagaaat attttttgct
     9961 ttttcaaggt acaaaaggcc cttttcgtat ttcttctctg agatcagggt atcgcccata
    10021 gccacatatg cggccatgat cttatcaagc ttggttacag cttctgcgtc attgataata
    10081 aaactgctgt aacttttttg ataattccgg gcatcagaaa tataaatttc agccagttcg
    10141 tattttccgt tatcgatcgc tttttgcgaa acgctaaggt aggactgata aataccaaaa
    10201 cgcgccgcag caaggctttt aacatgtttt tcaccacagg aatatccggg aagaccgtca
    10261 caaaaactta cacatttttc caaaacctca atagcttcat tatacttctc cagcttgtta
    10321 tattcattgc ctgtagccag cattctgtta taaacagagt cagtgtaaag gataacctga
    10381 tgataaatat caggggtcgg cttgagcgtg cgtaagatct gggaaatttt atcagcagcc
    10441 gcattcaggc tatcacaatg gtaaaggatc ttggccactt caagctgtga agggacaaaa
    10501 tcaggattat acaaaaccga acggttaaaa tacatttttg cttttgcagt ttccgctttt
    10561 tcaacttcag caaggccctt ttcataatag agcttgtcaa ggttttcgag tttcaggttt
    10621 agttgattcc tcgtgttgaa aagggtttct gaaagtttgt tgtatttatc aataaattga
    10681 acaggatcat agtccactaa atttaatttt ccgggaaatc ccttattata aagctcttca
    10741 gagttttttt caagttcttt caggctgata tcgtaaacaa tgatcatatc cgtattacca
    10801 taatcgatct cttttatttt tgctaaacat tgatcgatgg tcgttcccga taaaaaatat
    10861 tcatcaatca gtttaagttt tgagttaaaa cggctgatcg aattctcatc gtaatgaaaa
    10921 gcaaaatttt cgacacgtgt attgaatttt tttaatgctg atgaatctga aaatttatat
    10981 gaagccaaaa tgttatagcc ggggcttaat atcccatcgc ttacggtggt ttgataaagc
    11041 gggatcgctt tatctgaaaa aacgctaaaa gtaaaagagc aattagtagg gatcagaacg
    11101 tctgcgatgg aaaaaccttt ataaaaaaca tctccgttac aacctgcatc catgatctca
    11161 aaagtggctg aatattttcc gggttccgtt tcattgatac ggtcaatttt tctgcagtta
    11221 aaaaaatatt gtgtgtattc agtttttttg ggaatgctgc gcgctatttc actgataaaa
    11281 aaattggtat atttctggtt ttggtcagga gccgttgtgt aagaagactt caatgccatt
    11341 tcttcaatat tctggtatat catgctgttt tgtgcatttg caaaagcaaa tacaaataat
    11401 aaaacaactg aaagcagaat ttttctgaca aacattcctg aaataaaata ttacaaaggt
    11461 tattataaaa gctttcagtg cctttatatc tgtgaaaata atatcaacaa caaactgtta
    11521 atacaattta tgcagcacca gtgtttttag gctttacaga aaattctact tagcctttcc
    11581 tatacttgtt accttgaact ctgttctgcg gttcatctgg aatt
//
LOCUS       DAOPYB010000093        11548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6374_length_11548_cov_11.388410, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000093 DAOPYB010000000
VERSION     DAOPYB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6374_length_11548_cov_11.388410"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKH58_08115"
     CDS             complement(<1..451)
                     /locus_tag="PKH58_08115"
                     /inference="COORDINATES: protein motif:HMM:NF025696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNX89030.1"
                     /translation="MIPDFQSIMLPFLNLILDGQEHSTNEINDHLANYFNLTTDEINT
                     YLPSGSQKTFPNRVAWAKSYLKMAGLLETTKRGYFKITDSGISFLDTKPQEINIKVLK
                     TLPAFQENYGRSKEEESANDNENSHSKSTPEEILESSYLNIRKNLSQE"
     gene            complement(893..1723)
                     /locus_tag="PKH58_08120"
     CDS             complement(893..1723)
                     /locus_tag="PKH58_08120"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNX89031.1"
                     /translation="MRVELRYFTGTGNSLKILDTCREIFVRLNHETTISEINPDETNL
                     DKSDLLGFCFPVYAFGIPRICRKYLKSIHKFKDRQKVFVLITAGDADESGLSLKECRQ
                     ILSRKNCDIVYTGVIQMPINWITSPVPPYPPSKDEALEIITDGVEQAKSMAYEIGTGV
                     KKYHTFNFPKRYTKLRFYKDYWTFKYIGLPNLWRTFKVYDTCNGCQLCSKICPTKSIK
                     MIEKKPVWTSTCEQCMRCVNFCPNESIFQTMGGDTKGKNRYYEPDFKSKINNNHPHAE
                     "
     gene            complement(2021..3415)
                     /locus_tag="PKH58_08125"
     CDS             complement(2021..3415)
                     /locus_tag="PKH58_08125"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HNX89032.1"
                     /translation="MKKLALLPLLILLCLKANCQDLSLTPVVDERAELLSVVFNLAGS
                     QEFSNNTVAQYAQKVHDYFQPYIRHDVISLAEKLRKKRGVSYDAVMSMAIHISIRHDS
                     VVLITDAAKGSLDKRWGNSVGRFVFLLNDFYRKSNFHAFFSRNKPLYAEAVNRFSPIS
                     KAIDVEWFEKFFGEQSEDSFNLVLSFINGGNFGPNITHNNGRKDIYSIICAWNTDSLG
                     YPVYSGGVSEIIVHELCHSFCNPLGNKYYRDMKAVADSFYDINAGKMREQAYGNSLTM
                     TNEIFVRASTIRYFQEHGADEQKILNLIRFEQSKGFIWIEPLVSKLAEYENNRLKYLS
                     LNSFMPEIVRLQNSLSPSGFDKELAKDSPRIVSFSVENNSKNVDPCIREIIVVFDRPM
                     STRGISKGKGGNKNYPEIISSEWRDDKRTEMVIRVNLLPGKEYSLRFYKEFNQDKYGF
                     ALDRDYELNFRTGK"
     gene            complement(3554..4972)
                     /locus_tag="PKH58_08130"
     CDS             complement(3554..4972)
                     /locus_tag="PKH58_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX89033.1"
                     /translation="MKKRSLRLLLLSFLIIPCIATAQTVSSESAALKALNFMNSDAYH
                     LSDLTIQPLTYEGVQAAWLIRNTDKRFVIVSADSLLQPVLAYGDSIADMNAEEFSTWQ
                     KIFASDYKNRLDAVQNGYIPSQKARWAEPGSRLFEQWPPAGTTPTGGWLSTNWTQTSP
                     YNKLCPMDLNAGSRSIVGCPATAMAQICNYYRTTNNTQFSDNDDYYHNFGSGNNYWID
                     DAHAERDFPDFPKLNYWLDTIQKRYGLELPLSDSMKAALSFAAGVAAHQVYSASASGT
                     YGIDQAQMAYQRFNFDLSELIVPASFELRSRVAGDMKAAMVDHLGIVDAAVTVGHNVV
                     IDGYNTDEFFHFNFGWGGAYNGWYTLPPSGMPYSLNVIEGVVVNIKNTYADTKPVIKN
                     APSLNFAFRPENRLCISGIQGTGNYELKIFNAAGALVLTRTFAATKGAEVLLNIPGLS
                     SGIYLCRVQSATETFTGKAIKP"
     gene            complement(5355..7520)
                     /locus_tag="PKH58_08135"
     CDS             complement(5355..7520)
                     /locus_tag="PKH58_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89034.1"
                     /translation="MKNVILSTLFVAITLCASSQSYQMRETNRGKNYLIKNSNGVTQA
                     VRSKSRTENIKHEMIFPASFVQVQTADAFSGKNPERNVLTHHLTLHFNKNMDFDGNVH
                     IVSSSCYYDDYDLTWTEGGTKAEIDLPEDHYEIISSYDDFFNTANYYYVFMHALNINQ
                     DVDTTVNFEEMAPHSLVMRCMDENNQQIEKDDPTVMQKGVTFDIEFPEGFVFRSSLLQ
                     GDIANGYKFSDVNEGFKIITNQFFVRPGGLYITDFPQLNGLDHDTTLINDPSDYKRMQ
                     MRMHASPAASPENYITFGYGSVYNLYGFYMCSLNFGMYQNYPYQNNDTVMVFANNHEN
                     PSNAMTATVANSLLWEEDPLAFNFKFMKSDAFFISPNDSIVFTSFNTSAATPKYPDKC
                     MVDLGNTAPCNFVTFRNNQYADNSIFCFSNSYGGNNEIREIDGHQSIYEIRKGEEILV
                     QDTLKNFMDPFTISGQGVYTFSISNNNYTISGQSGQSNFEATFNTGNEDANPPVLSSF
                     KLLSADKSITNHFFNTSSGSILLSSFDLDTETLKVTKPSSVEVYYKNFNDSAWISLGS
                     VENTALFDSSYYGSFYACDLSPVLSQFSASGYLDIKIVIVDAAGNAAMQSLHPAAFVE
                     NNVGVPQPAERKKCHLQVYPNPTAGSSIVSFTLAKDTRSVLSVYSMNGRLTEIIQDGM
                     MEKGSHQLNWNASAKLSPGIYLLKLETEAAVETTKVTIR"
     gene            complement(7673..9526)
                     /locus_tag="PKH58_08140"
     CDS             complement(7673..9526)
                     /locus_tag="PKH58_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX89035.1"
                     /translation="MHSENLSKLRLILHHLNLYLRSNKKKAIFTGSGILLLIILSVLF
                     FIRIRHTPDQKEHNIAKQRLSAIFSELDTAFWKNKDSTLLLSREAYQIATGIGDSNSM
                     AEALYNEAGALRETPLDDSSLVLYNRALGMANAVHNDSLKARIKNCIGNYYFFKDNYY
                     LATRYFTEAREIADSLKDNTAIGVYSNGLGLVNLALGNLDTAVTYFKRTAVVCEKTGN
                     LRDMSGALMNTGNCYIEKKDFPEASAFLKKALVIAEKVQDHELIAKVYLSLGMIRLYS
                     NEGKNGKPAASDYFFKALTASKEAGNIKITGLAYQDLGSYYIENNQLDKARSYFNESL
                     SIFSTTGAKSYEMRANLALSEVNKRQGNWQEAYRYYIRFVGLNDSIQNRSIQKKVSDY
                     QYEIESQKKEYEKQRLQDKISGQRKGNLFLTGGVIFLIILAVVIRSNLKKSIMLQKQE
                     NAHLQERVEMADRIKKLELTRHQVEMDAKNKEMVSFSLQLTSKNDLLNQISKLSEKYY
                     NAENLDKAYYNELTKVIESNLDADIAWGQFKELFENVHLGFFNRLRQSWPVLTENDLR
                     FCAYIRINLRPKEIAKILNIGADSVKTYRNRLKKKFGLDINDSLDDFIRNI"
     gene            complement(9764..11323)
                     /locus_tag="PKH58_08145"
     CDS             complement(9764..11323)
                     /locus_tag="PKH58_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89036.1"
                     /translation="MKTLNFLLLTIVTLLLSFSTSFGQSDCLIAWYKLDGNAFDNSGN
                     NNNGILTGATPTSDRWGNANSALKFNGIYDKVDLPGDFDFPERTISVWFKTNSFPTSG
                     AWIYDSDHSNLMYGHTQIYVISESGINKISLCVGYNTSSYTFDNALTDTWYHAAIVVN
                     ENYVKYFINGALIDSVPNNDFAHSSDGDNFAKIGTTRKNDRFFDGAIDDVRIYNCALS
                     DEEIQTLYTYGNDCIYAWYKLDGNAFDYSGNNNNGILTGATPTSDRWGNANSALKFNG
                     IYDKVDLPGDFDFPERTISVWFKTNSFPTSGAWIYDSDHSNLMYGHTQIYVISESGIN
                     KISLCVGYNTSSYTFDNALTDTWYHAAIVVNENYVKYFINGALIDSVPNTNFAHSVNG
                     DSFAKIGTTRNNDRFFDGVIDEVRIYNCAISNAEIDSLYDINVLINKIITVNGLSVYP
                     NPASNYITVAMDVPEESVIEILNIESQLIKKMEVKGTKSIIDISNFPNGIYIIKATTD
                     NGIITKKFIKD"
BASE COUNT         3115 a         2425 c         2374 g         3634 t
ORIGIN      
        1 attcttgaga caggttctta cgaatattaa ggtaactgct ttcaagtatt tcctctggag
       61 ttgattttga gtgagaattt tcattatcat ttgcagattc ttcttcctta gatcggccat
      121 aattttcttg aaacgcaggt aatgttttta atacttttat attgatttct tgaggtttag
      181 tatcaagaaa acttatcccg gaatccgtaa ttttgaagta cccacgtttt gttgtttcaa
      241 gtaatcctgc cattttcaag tatgactttg cccatgctac tctattggga aatgtttttt
      301 gagatccgct tggtaaatag gtgttaatct catctgttgt aagattaaaa tagtttgcta
      361 aatgatcatt tatttcattt gtagaatgtt cttgtccatc cagaattaga ttaaggaatg
      421 gaagcattat tgattgaaaa tctggtatca tatcgttgat ctatgttttt cttatggttg
      481 gcgataacgt gtaggtttat gaaataattg ggatgccctt cacttcctct gtccgattgc
      541 tccactaata tatctgcttt ccagctaata atactttatc ggacaggctt ttattatcat
      601 gttgcatctt cccgtttatt attgatgtag ccctcactat catttcaggg cctgtcattt
      661 tccggtcctg aagttcagtt cataatctct atccagcgca aaaccatact tgtcctggtt
      721 aaactcctta tagaatctca gggaatactc cttaccggga agcaaattca cccgtattac
      781 catttcagtc cttttgtcat cccgccattc gctggaaatt atttccggat aatttttatt
      841 tcctcctttt cccttgctga tgcggcgggt accctgctat ccggcgcaat ttttattccg
      901 cgtgtggatg gttgttattt atcttggatt tgaaatcggg ttcgtaatat ctgtttttcc
      961 ctttggtatc gcctcccata gtctggaata tcgattcgtt cggacaaaaa ttaacacatc
     1021 tcatacattg ctcacacgtt gacgtccaaa caggtttctt ttcaatcatc tttatacttt
     1081 ttgtgggaca gatttttgaa cataactgac aaccattaca agtatcatat accttgaatg
     1141 ttctccacaa attaggtaat ccaatgtatt taaatgtcca ataatctttg taaaaacgaa
     1201 gtttggtgta ccgtttagga aaattaaatg tatgatattt ttttacaccg gttcctatct
     1261 cgtaagccat gctttttgct tgttcaacgc cgtctgtaat gatttcaagt gcttcatctt
     1321 ttgatggcgg atagggcggt accggtgaag tgatccagtt tatcggcatt tgaatgactc
     1381 ccgtataaac aatatcacaa ttttttctgc tcaaaatctg gcggcattct ttaagcgata
     1441 atccggattc atcagcatcg ccggccgtaa ttaaaacaaa taccttttgc cggtctttaa
     1501 acttatgaat ggactttaaa tattttcgac aaattcgagg tattccaaat gcatatacag
     1561 ggaaacaaaa acctaacaag tctgatttat caagatttgt ctcgtcagga ttaatttctg
     1621 aaatcgttgt ctcatgattt aaccggacga aaatttctct gcatgtatcc agtattttta
     1681 acgaattgcc tgtaccggta aagtatctta attcaactct catttgatca gtttacggcg
     1741 tttttattaa tttgtcacaa cgttgacggc atggctggta agggatttcg tgtaatttcc
     1801 tgtcacgttt cacgaagaat taaagcggcc taacgagtag gttttcgaag tatttcgctt
     1861 atttctttaa taagaagaat aaccgcaacc ccatttatcc gatcggcccg ctaataaacc
     1921 tgctttccag ctaataatac tttaacggac aggcttttat tatcatgttg catcttcccg
     1981 tttattattg atctaaccct cactatcatt tcagggcctg tcattttccg gtcctgaagt
     2041 tcagttcata atctctatcc agcgcaaaac catacttgtc ctggttaaac tccttataga
     2101 atctcaggga atactcctta ccgggaagca aattcacccg tattaccatt tcagtccttt
     2161 tgtcatcccg ccattcgctg gaaattattt ccggataatt tttatttcct ccttttccct
     2221 tgctgatgcc gcgggtactc atgggcctgt cgaaaacgac gataatttcc ctgatgcagg
     2281 gatcaacatt tttactgtta ttttcaaccg aaaacgagac aatccttggg gagtctttgg
     2341 ctaattcctt gtcaaaaccg gagggcgata aagaattctg caaccggaca atctcaggca
     2401 taaagctgtt cagggacaga tatttcaggc ggttgttctc atactcagcc agtttgctaa
     2461 cgagaggctc tatccatata aatccttttg actgttcgaa acggatcaga ttcaggattt
     2521 tttgttcgtc agcgccgtgc tcctgaaaat atctgatcgt cgatgccctg acaaaaattt
     2581 cgtttgtcat cgtcagcgag ttgccataag cctgttctct cattttgccc gcattaatat
     2641 cataaaagct gtcggcaaca gccttcatgt cgcggtaata tttgtttccc aaaggattac
     2701 aaaacgaatg acagagctca tgcactatga tttctgaaac gccgcccgaa tatacgggat
     2761 agccgaggct atcggtgttc caggcacaga tgatggaata aatgtctttc ctgccgttat
     2821 tatgggtaat gttcggaccg aaatttccac cgttgatgaa actaagcacc agattgaagc
     2881 tgtcttcgga ttgttcgccg aaaaattttt caaaccattc aacatcaatg gcttttgata
     2941 tcggcgaaaa gcggtttact gcctcagcat aaagcggttt gttccggctg aaaaatgcat
     3001 ggaagttgct ttttctgtaa aagtcattca gcagaaaaac gaatctgccg acgctattac
     3061 cccatctttt atcaaggctg ccctttgctg catctgtgat cagcacaaca gagtcatggc
     3121 gtatgctgat gtgaatggcc atggacatca ctgcatcgta tgaaacccct ctttttttcc
     3181 ttagtttttc agccagactg ataacgtcat gccgtatgta tggttggaag taatcgtgaa
     3241 ccttctgagc gtattgtgcc accgtattat tagagaactc ctgcgatccg gccaggttga
     3301 aaaccacact cagcagttcg gccctttcat caacgacagg cgttaaggac aagtcctggc
     3361 agttagcttt taaacagagt aaaatcagca gtggtaagag ggcgagtttt ttcattggtt
     3421 aaatattgat atgccggagc acgcaacgcc tccggcaatt ctctttctgg ttacacactt
     3481 ataccataca gcgtatacaa tagtttacag ccgtgagaaa tctcttcctg acaagtaatg
     3541 ccgggtaacg gtatcagggc tttattgctt tcccggtgaa agtctcagtc gcagattgaa
     3601 cccgacacag ataaatgccg gaacttaatc cgggaatatt cagcaatact tcagcgcctt
     3661 tagtggcggc aaatgtcctg gttaaaacca atgcgccggc agcattgaat atcttcagct
     3721 catagttgcc cgtgccttga atgccggaaa tacagagccg gttttccggc ctgaaagcga
     3781 aattcagtga cggtgcgttt tttattacag gttttgtgtc ggcgtaagta ttcttaatat
     3841 ttacgaccac gccttcaata acattcaggc tgtaaggcat tccgctcgga ggcagggtat
     3901 accatccatt ataggctccg ccccatccga agttgaaatg aaaaaactcg tcggtgttgt
     3961 agccgtcaat cactacattg tgccctaccg tgacagcggc atccacgatt cccaggtgat
     4021 cgaccatggc tgccttcatg tctccggcca cgcggcttct gagttcaaaa cttgccggga
     4081 caatcaactc ggagaggtca aaattaaaac gctgataagc catttgcgcc tggtcaattc
     4141 cataagtccc ggaagcagag gccgaataca cctgatgggc ggccacgcct gctgcaaagc
     4201 tgagcgctgc cttcatgctg tcggaaaggg ggagttccag gccgtacctt ttttggatcg
     4261 tatcgagcca gtaattcagt ttcgggaaat cggggaagtc gcgttctgcg tgagcatcat
     4321 caatccaata gttgttgccg ctcccgaaat tgtgataata atcatcatta tcgctgaatt
     4381 gggtattgtt tgtcgtcctg taatagttgc atatctgcgc cattgccgtt gccggacatc
     4441 ccacaatgga acggcttccg gcattcaggt ccatcgggca cagcttgttg tatggcgaag
     4501 tttgtgtcca gtttgtgctg agccagcctc cggtgggcgt tgttccggca ggtggccact
     4561 gctcgaaaag cctgcttccg ggttcggccc atcgtgcttt ttgagacgga atatatccat
     4621 tttgaacggc atcaagcctg tttttataat ctgaagcaaa gatcttctgc caggttgaaa
     4681 attcctcggc attcatatcg gcaatgctgt cgccgtatgc aaggacgggc tgcaggagtg
     4741 aatcagcgct tacgatcaca aaacgcttgt cggtattcct gatcaaccag gctgcctgca
     4801 ccccttcata ggttaagggc tgaatggtaa gatcgcttag gtggtaagcg tctgagttca
     4861 tgaaatttaa tgcttttaaa gcggccgatt cactgctgac agtttgggcc gttgcgatgc
     4921 agggaatgat caggaagctt aaaagcagga gccggagtga tctttttttc atttttaaaa
     4981 catgttaaaa atcaggatgt caaatgtaca aaaccgttta ataccggcaa atttatttat
     5041 cggcgtaata gtttctcctg caaccaggaa ttgttcactt caaaattttt gatattgaca
     5101 aaggaaccag gtctgtacca gcgtgattat aaaatgtcaa aatacagggt cgggcttgtt
     5161 atttgcctct ttttattttc atgccgttgc aaccggaatt tgatttaata aatacctgcg
     5221 gatatttttc ttctatggtg taagtcaaaa taacaacagg gacacagcgt actgtgtccc
     5281 tgtccggact cttgccgact tttgtattta cctgcggctg aactgattat tccgccgaag
     5341 ccagtgtgag ggaattatct gatggtaacc ttcgttgttt caactgcagc ttcggtttcc
     5401 agcttaagca gataaatgcc gggtgacaat ttcgctgatg cattccagtt caactggtgg
     5461 ctgcctttct ccatcatccc gtcctgtatt atttcggtta aacggccatt catgctgtac
     5521 accgaaagga ctgatctggt gtcctttgca agggtaaagg aaacgatgct gcttccggct
     5581 gtgggattgg gataaacctg caagtggcac ttcttccgtt cggcaggttg cggcacaccg
     5641 acattgtttt caacaaatgc ggcggggtgc agcgactgca tagccgcgtt gccggctgcg
     5701 tcgacaataa caatctttat atcaaggtat cctgatgccg aaaactgaga caacacaggt
     5761 gaaaggtcgc aggcgtaaaa actcccgtaa tacgaactgt cgaacaaggc cgtgttttca
     5821 acgctgccga gtgaaatcca tgctgaatca ttgaagtttt tataatagac ttctaccgag
     5881 gaaggtttcg tgactttcag ggtttctgta tccagatcaa atgagctcag caggatactt
     5941 cctgatgagg tgttgaagaa atggttcgtg attgatttat cggcagataa gagtttaaaa
     6001 gaggataata ccggagggtt ggcgtcttca tttccagtgt taaatgtggc ttcaaaattg
     6061 ctttgcccgg attgaccgct gatggtataa ttgttattgg atatgctgaa tgtatatacg
     6121 ccctgcccgg aaatagtaaa tggatccatg aaatttttaa gcgtatcctg tacaaggatt
     6181 tcttctccct ttctgatttc atatatgctc tgatggccat ctatttcacg gatttcgttg
     6241 ttccctccat acgaattgct gaaacagaaa atcgaattgt cggcatattg attattcctg
     6301 aaagtaacaa aattacaggg cgcggtattg ccaagatcaa ccatgcattt atcgggatat
     6361 ttaggtgtgg cggcagacgt attaaaagag gtgaagacga tgctgtcgtt cggcgaaatg
     6421 aaaaacgcgt cggatttcat aaatttaaaa ttaaaggcca gaggatcttc ttcccacaga
     6481 agggaattcg caacggtagc tgtcatcgca ttgctggggt tttcatggtt gttcgcaaaa
     6541 accatgaccg tgtcattgtt ctggtacgga tagttctgat acattccgaa atttaaggag
     6601 cacatataga acccgtaaag gttataaaca gagccatatc caaatgtgat ataattctcc
     6661 ggggaagctg ccggagaagc gtgcattcgc atttgcatcc gtttgtagtc agacggatca
     6721 ttgatcaaag tggtatcgtg atccaggccg ttcagttgag ggaagtcagt aatgtataat
     6781 cctccgggcc ttacaaagaa ttgattggtg atgattttaa atccctcgtt gacatctgaa
     6841 aatttatagc cgttagcaat gtcgccctga agcagcgaag agcgaaatac aaaaccctcg
     6901 ggaaactcaa tatcaaaagt gacccctttt tgcataacag tgggatcgtc cttttcaatt
     6961 tgttggttgt tctcgtccat gcagcgcatc accaggctgt ggggtgccat ttcttcaaaa
     7021 ttaacggtcg tgtcaacatc ctggttgatg ttcaacgcat gcataaaaac ataataatag
     7081 tttgccgtat taaagaagtc gtcgtaggat gatattatct cataatgatc ctccggcaga
     7141 tcaatttcag cttttgtgcc tccttcagtc caggtcagat cataatcatc ataatagcat
     7201 gaactgctta caatgtgtac gttgccgtca aaatccatat ttttattgaa atgtaaggtg
     7261 agatgatggg ttagtacatt gcgttccggg ttcttacctg aaaatgcgtc tgctgtttgt
     7321 acctgtacaa atgaagcggg aaagatcatc tcatgtttga tattttctgt ccggcttttg
     7381 ctccgaaccg cctgggtgac tccattgcta ttttttatca gataattctt accccggttt
     7441 gtttctctca tctgatagga ctgagaggat gcacataacg ttattgcaac aaaaagtgtc
     7501 gacaatatta catttttcat ggtcactgat ttttgagttg ataactgttg gattagtaat
     7561 cgatcgaagc gccaaatcta tcgcataaca tgaaatattt aaaaatccgg gggttatcaa
     7621 atgggtaaca actaaaaaaa aacggttgtt tctgatgcca tcatccggtc tgtcagatgt
     7681 ttctgataaa gtcatccagg ctgtcgttaa tatccaatcc gaatttcttc ttcagacgat
     7741 ttctgtatgt tttgacggaa tcggccccaa tgttgagtat tttggcgatt tctttcggcc
     7801 ggagattaat cctgatgtag gcgcagaaac gaaggtcatt ttcagtaaga acaggccatg
     7861 actgccttaa ccggttaaag aaacccagat gcacattttc aaacagctct ttaaactggc
     7921 cccaggctat gtcggcatca agattgcttt caattacttt ggtcaattca ttgtaatagg
     7981 ctttatcaag gttctcagcg ttgtagtatt tttctgatag cttcgatatc tggttcagca
     8041 aatcattctt ggaagtaagc tgcaatgaaa aactcaccat ctccttgttt ttggcgtcca
     8101 tttcaacctg atgccgggtt agctcaagtt tttttatcct gtcggccatt tctactcttt
     8161 cctgcaaatg ggcattctcc tgcttctgca gcataatgga ctttttcagg ttgctccgta
     8221 taaccactgc taaaatgatg aggaatatga cgcctccggt cagaaaaagg tttccctttc
     8281 gctggccgct gattttatcc tgcaaacgct gcttctcata ctccttcttt tgggattcaa
     8341 tctcatactg atagtcggat acttttttct gaatgctccg gttctggata ctatcattaa
     8401 gtccgacaaa cctgatataa taccggtaag cttcctgcca gttgccttgc cgtttattta
     8461 cctccgataa cgccaggttg gccctcattt catagctttt ggccccggtt gtggagaaaa
     8521 tggaaaggct ttcattaaag tagcttcttg ctttatctaa ttgattgttt tcaatatagt
     8581 aagagccaag gtcctgataa gctagaccgg taattttaat atttccggct tctttcgagg
     8641 cggttaacgc tttaaaaaaa tagtctgaag cagcaggctt tccgttttta ccttcattgc
     8701 tgtacaacct gatcattcca aggctgaggt aaacctttgc aatcagttca tggtcctgta
     8761 ctttttcagc aattaccaat gcctttttca gaaaagccga agcttccgga aagtcttttt
     8821 tttcgatata gcaattgccg gtattcatca acgcgcctga catatccctg aggtttcccg
     8881 tcttctcaca gaccaccgca gtacgtttaa aataagtaac cgcagtatcc agattgccca
     8941 atgccaggtt gaccagtcct aagccgttgg agtaaactcc gatggcagta ttgtccttca
     9001 gactgtcagc tatctcccga gcctcagtaa aatagcgagt ggcgaggtaa tagttgtcct
     9061 tgaaaaagta ataattcccg atacaattct tgatccttgc tttcaaagaa tcattatgca
     9121 ccgcattcgc catacccagc gctctgttgt ataaaacaag gctgctatcg tccagcgggg
     9181 tttcccttaa ggcgccggct tcattgtata aggcttcggc cattgagtta ctgtcgccaa
     9241 tccctgtggc tatctgatag gcttcgcggc tgagcaataa ggtagaatct ttattcttcc
     9301 aaaacgcagt atccagttca ctgaaaatcg ctgataagcg ttgttttgcg atattatgct
     9361 ctttttgatc aggtgtatgc cttatgcgga tgaaaaagag gactgacaga atgatcagta
     9421 acaggatacc actacctgtg aaaatcgctt tttttttatt cgaacgtaag tacaggttaa
     9481 gatgatgaag tataaggcga agcttagaga gattttcaga atgcatgacg gaaatttaca
     9541 acaaagaaaa tgaaaaagct tattcaaaaa aatccggttc tctcctgcga acaggaaaga
     9601 ttcaccttga aaatttctgc ttttgagaaa aattgtggtt tatacctgcc cggttgatca
     9661 gttgccggta taaagccggg aagcgccgga ctcatcgccg ggctcccgtt cctggaaagc
     9721 tatgacagac ctcccatatt cctgcttgtc gtaaccggta aatttaatct tttataaatt
     9781 tctttgttat aataccattg tctgtcgttg ctttgattat gtaaattcca tttggaaaat
     9841 ttgaaatatc aatgattgat tttgtgcctt ttacttccat ttttttgatc aattggcttt
     9901 caatgtttag aatttcaatt actgattctt caggaacatc catagctaca gtaatataat
     9961 tactggcagg atttggataa actgataaac cattgacggt tatgatttta ttaatcaaaa
    10021 cgttaatgtc ataaagcgaa tcgatttcag cattacttat agcgcagtta taaattctta
    10081 cttcatcaat caccccgtca aaaaatcggt cattatttcg ggtagttccg atttttgcaa
    10141 aactgtcgcc attgacggaa tgagcaaagt tggtgttggg aacagaatct attaaagccc
    10201 cattaatgaa atatttaacg tagttttcat ttactacaat agcggcatga taccaggtat
    10261 ctgttaaagc attatcaaac gtgtatgatg aagtattata accaacacaa aggcttatct
    10321 tgtttattcc gctttctgag ataacatata tttgggtatg accatacatt aaatttgaat
    10381 gatcagaatc atagatccat gcaccagaag ttggaaacga gttcgttttg aaccaaacgc
    10441 ttattgttct ttcagggaag tcaaaatcac cgggaaggtc aactttatcg taaatgccgt
    10501 tgaatttcaa tgcactattt gcattgcccc aacgatcaga agttggagta gctcctgtca
    10561 gaatcccatt attattatta ccgctataat cgaacgcgtt gccatcaagt ttataccagg
    10621 cataaatgca atcattacca tatgtgtaca gagtctgtat ttcttcatcg ctcaacgcgc
    10681 agttataaat tcttacatca tcgatcgccc cgtcaaagaa tcggtcattt tttcgggtag
    10741 ttccgatttt tgcaaaattg tcgccatcgg atgagtgagc aaaatcattg ttgggaacag
    10801 aatctattaa agccccatta atgaaatatt taacgtagtt ttcatttact acaatagcgg
    10861 catgatacca ggtatctgtt aaagcattat caaacgtgta tgatgaagta ttataaccaa
    10921 cacaaaggct tatcttgttt attccgcttt ctgagataac atatatttgg gtatgaccat
    10981 acattaaatt tgaatgatca gaatcataga tccatgcacc agaagttgga aacgagttcg
    11041 ttttgaacca aacgcttatt gttctttcag ggaagtcaaa atcaccggga aggtcaactt
    11101 tatcgtaaat gccgttgaat ttcaatgcac tatttgcatt gccccaacga tcagaagttg
    11161 gagtagctcc tgtcagaatc ccattattat tattaccgct attatcgaat gcgttgccat
    11221 caagtttata ccaggcaatt aaacaatcag attgaccgaa tgaggtgcta aatgataaca
    11281 gtaatgtcac gatggtgagc aagagaaaat ttaatgtttt catttcttta atttttataa
    11341 attaacatat tttatttact ataacgttgg tggtctgaaa agttgtcgtt tgcgggtgat
    11401 gtccgatcag gcaattgtga acttaatggc gaacagcaac cctttgatga cttactgtca
    11461 cgtaaatttt ttatactgct aaatgaactg aatccgactt ataccagata gttcattcaa
    11521 agataaccaa tttttccacg attaggaa
//
LOCUS       DAOPYB010000094        11469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6435_length_11469_cov_9.542316, whole genome shotgun sequence.
ACCESSION   DAOPYB010000094 DAOPYB010000000
VERSION     DAOPYB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_11469_cov_9.542316"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..3446
                     /locus_tag="PKH58_08150"
     CDS             54..3446
                     /locus_tag="PKH58_08150"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX89037.1"
                     /translation="MKKLFLLILFFLPNLLTAQLIINELMTNNVSALMDESYNYSMWV
                     ELYNTSSTTSYNQTVYYFTDDLTQPRKWCPVSKLINPRGFSVLWFERPDRTGHANFKL
                     DPKGSTLYLLNAFGQIIDRVSYPEQYRNISYGRKTDGGSEWVYFDQHSAGKTNNGKMS
                     ASKRCAKPEFNATGGFFSTPIYISLATPAPGDTIYYTLNGAEPTRSHTRYTPGTSINI
                     NKTTIIRARCFSAGKLTSDIASSTYFIGERNFKLPVVSIVTEQANLTDNTIGIYVVGT
                     NGITGNGMNSPVNWNQDWDRPANIELYDTTNNVRINQEIDIRIAGGWTRMNGQKSLVL
                     NPTLKYGNNKFDYDIFKATKPGMKYRSIMLRNSGNDFDHSMMRDGFMQSLVMKRMNLD
                     YLAYEPAVCFMNGVYYGIQNLRERSDEDFVYSNYGYNEDEIILLESWKMNTDPEFLKL
                     SNYITNNDITKNSVYDQVCEMMDMDNFMDYFLTQIYLRNTDWPHNNVKAWKKKDGGKW
                     RWILYDTDFGYNLWNNDHTHNTLTWALGEQAGTLPVDAPWSTLLLRRLVLNETFKKRF
                     IDKFAIQMSSTFEANRSNAILDSLAAKISKEIVYHKNKWGSGRDFNTDIATMKGFSFN
                     RQNSMMNFIGARFLNNPGTAEIQISSNNPKATYQYNSEIIQDAQINLKHFKNQTYSLE
                     ANAVPGYTFKQWEIKGNSGSQLLIANGSIWKYSDGNGVPASNWFSPGYSDSGWKSASA
                     ALGYGALGQTTTIGYGTDANNKYPTAYFRKNINITNLASKENFVISTFVDDGAVVYVN
                     GTEVERINMPAGVITFNTYSATYNNGITGNFTVPKNLLNEGNNVIAVEVHQTSANSSD
                     LIFNLQLTCNIAEETQVVLSPVYNSVLTTTRSIKAIYEKNSGEDPDKDLNVVINEVVA
                     SNTLAADENEDKDDYLEIYNNGSQAVNIGGWYVSDTPDNPTLYQIPGTNPALTTIPAK
                     GRLVIWADGEPLQGILHADFKLSKDGEKVTLSRLNYLGELTEVDAVTFPYMEQNMSYS
                     RVPDGSSTWYVTTPTCNLTNGNWSATEDIRMTARIYPTLVDNRFTVENARAGYVQVVD
                     LTGKILLTQECNTETTVVEVSHLQPGVYILIAGTEKFKFIKK"
     gene            complement(3893..5713)
                     /locus_tag="PKH58_08155"
     CDS             complement(3893..5713)
                     /locus_tag="PKH58_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNX89038.1"
                     /translation="MRNIELLSPAKNLECGLAAINHGADAVYIGAPMFSARAAAGNNI
                     ADIEQLISYAHRYRSKVLVALNTVLTDRQLTEAEKLIHQLYEIGTDALIVQDMGILQL
                     DLPPIELHASTQTDNRTVEKVRFLQDVGFSRVVLARELTLRQIREIHDNTNVELECFI
                     HGALCVSYSGQCYISQAMTGRSANRGECSQYCRLPYQLTDSDGQVLVKNKHLLSLKDL
                     DLSDHLDELIDAGISSFKIEGRLKDADYVKNITAFYRKKLDAILECRKDVRKSSSGTT
                     TFFFEPNPDKSFRRSATDYFLNGRQQNISQPETPKSIGEEIGTVTYISGQFIEIKTEK
                     EIHNGDGLCFLTRDGILKGFRVNRVEGRKVFPAEMPEMDRNTFLYRNHDQEFDKILAG
                     KTSERKIGVNILFSEKPDGFSIQITDEEGLSAISEFVSEKQAANNAERAIQTIESQLT
                     KLGNTIYMARNVDISVNSPWFLPASVLNEWRRQAIELLDEKRREAYVTGKRRPANSEA
                     AFPEKKLTYLGNVTNERAREFYLQHGVEEVMPGFEVQAEPGVPLMFCRHCIKYSLGWC
                     PKEGYKATFKEPLYISYKDQQFQLEFDCRKCQMLVLKRKS"
     gene            complement(5721..6638)
                     /gene="metA"
                     /locus_tag="PKH58_08160"
     CDS             complement(5721..6638)
                     /gene="metA"
                     /locus_tag="PKH58_08160"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNX89039.1"
                     /translation="MPLNIPNTLPAVDALRKENIFVMDTERAQSQEIRPLKIVILNLM
                     PLKITTETDLIRLLSNSPLQIEIDFLRLKSHTSKNTPIEHMMAFYKNFDDIRKSNYDG
                     MIITGAPVEQLEYEEVKYWKEVTDIFDWAHYHVTSTLYICWAAQAGLHHFYNIPKYPL
                     PEKMFGVFEHKIYDKLNPIFRGFDDVFYVPHSRHTEIRREDILKNPELTLLSESEESG
                     VYMVMARNGRDFFITGHSEYSASTLDTEYKRDVKKGLPIKMPVNYYRENNPANEPVVL
                     WRSHANLLFTNWLNYFVYQETPYDLANIQ"
     gene            complement(6676..7464)
                     /locus_tag="PKH58_08165"
     CDS             complement(6676..7464)
                     /locus_tag="PKH58_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNX89040.1"
                     /translation="MVAGMILVMTLTLNMSRKGHIFNEQRDLSEIRQSGRLRVVTEKS
                     SLGFKLYNDQIEGFQYELVKAFADSLGLELEINPENNIDSAIAGILENRYDLIAMNLP
                     NTSEIKKKLAVSIPIISGRQMLVQQEIIDTTNVKAIRNHHLLTEDSICIPVRSPFRQR
                     LENLSNEIADSIKIVEIPDKSTEDLVQMVSAGKIKYTICDELQAKRLKRIFTNIDISM
                     PVGFTQQFSWGVNPSAGQLRDSLNIFLNDFLMSNDYWKIYRKYY"
     gene            complement(7513..9420)
                     /locus_tag="PKH58_08170"
     CDS             complement(7513..9420)
                     /locus_tag="PKH58_08170"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNX89041.1"
                     /translation="MKRYILIYSLVILGMQAFPQTDSISSQKKTPAKPVKHIKSWTIS
                     EQMAVADTVPMDTATINFPDQNPVESFSIANSFSGNLGSPLQSKLYFKRPQGSDFLFD
                     EAYKPYTLDISSALFYDATYPYTNLTYLTGGTAYRKEEQVKFLFTASPSKKANFGTTL
                     DYIHAVGEYDNQAVKRFSGGIFGRYNGKHYSGNGLIAVNNHFNYESGGLSDPSYLTNP
                     DISVKPQDMPVFLKGYSVFRKNQAFYNHSYSIGFNREVKVNKDSVRYDYVPVTRFGHM
                     IKLEEMKKRYYEPTLVTDFYKFTDPVKKDANDTAAVRTLTNLFSISLEEEFNKWMKFG
                     MTGYVENEIQQFTYEPDTTLAWTTKSNTRVGGILSKNQGTRFRYNVLGDIYLIGYKLG
                     EFRIEGKATGNFNLFNQNIQLTANAFVRNEEPAYFLQHYYSTHFKWDNEFSKMYKTHI
                     SGSFSLPKLYTLLNVGVENITDYIYFNENALPTQYNGNVQVLSADLKQDFHFGKFVLE
                     NNAVYQVSSVPDVLPLPTLTLFHNFYYYSKWFKDLYPQIGANVRYHTNYYAPAYMPAT
                     GQFYTQKEVLIGNYPILNVYANFHLKQSRFFIEYNHLNKYFMSGAHFYMPNYAINPPV
                     LKLGLSWNFYN"
     gene            9724..10116
                     /gene="rpsF"
                     /locus_tag="PKH58_08175"
     CDS             9724..10116
                     /gene="rpsF"
                     /locus_tag="PKH58_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNX89042.1"
                     /translation="MLNHYEAVFILTPVLSDVQMKEAVDKFKAVITDNGGTITNEENW
                     GMRKLAYPIQKKSTGFYALLQFDVEPTVIATLETQFRRDEKVLRFLTFRLDKYAYEYA
                     EKRKNVKSQPKEVKEEEAKKETKEEKED"
     gene            10120..10389
                     /gene="rpsR"
                     /locus_tag="PKH58_08180"
     CDS             10120..10389
                     /gene="rpsR"
                     /locus_tag="PKH58_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNX89043.1"
                     /translation="MAAHSGEIRYLTPPTVDVKKKKYCRFKKSGIKYIDYKDPEFLKK
                     FLNEQGKILPRRLTGTSLKYQRKVAQAVKRARHLALLPYVTDMMK"
     gene            10408..10851
                     /gene="rplI"
                     /locus_tag="PKH58_08185"
     CDS             10408..10851
                     /gene="rplI"
                     /locus_tag="PKH58_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNX89044.1"
                     /translation="MEIILKEDVINLGYKGDIVKVKDGYGRNFLIPTKKAVLATESAK
                     KMLAEDNRQRAHKLEQIKNTALELAEKLKEVSLTIGAKTSSTGKIFGAVGPIQVSESL
                     EKAGLTIDRKIISIKEPVKEIGSYQATIKLHKEVSVEIPFEVVSE"
BASE COUNT         3540 a         2386 c         2282 g         3261 t
ORIGIN      
        1 aaaacaaaaa aaaacacagg ctttctcaat tttttgccat cattgttgct accatgaaaa
       61 aattatttct tcttatattg ttctttttac ctaatttgct taccgctcag cttattatca
      121 acgagttgat gaccaataat gtatcggctc tgatggacga aagctataat tattcgatgt
      181 gggtggaact ctacaacaca agtagtacca cttcatataa ccaaaccgtt tattatttta
      241 cagatgacct cactcaaccc agaaaatggt gtccggtgtc aaaattaata aatccccgag
      301 gtttcagcgt actgtggttt gagcgacccg accgtaccgg acatgctaat tttaagctgg
      361 atcctaaagg aagcaccctt tatctgctga atgccttcgg acaaattatt gaccgggtga
      421 gttatcccga acagtaccgc aacatatctt acggcaggaa aacagacgga gggagtgaat
      481 gggtttactt tgatcagcac agcgcaggaa aaaccaataa tggaaaaatg agcgcttcga
      541 aacgttgtgc aaaacctgaa ttcaatgcaa ccggtgggtt tttctccacc ccaatctaca
      601 tttcactggc tacacccgct ccgggcgaca ccatttatta cacccttaac ggcgcggaac
      661 ccacaagaag tcatacccga tacacaccgg gtacttcaat caacatcaat aaaaccacaa
      721 ttatcagagc acgatgcttc tcggctggca aacttaccag cgacatagcc agtagcacct
      781 atttcatcgg cgaacgcaat tttaaattgc ctgttgtatc cattgtcacc gaacaggcaa
      841 acctgaccga caataccatt ggtatatatg tggttggaac aaacggaata acaggaaacg
      901 ggatgaactc acctgttaac tggaatcagg actgggaccg acctgcaaat attgaactgt
      961 acgacacgac aaataatgta aggattaatc aggaaattga catacgcatt gcaggcgggt
     1021 ggacccgtat gaacggtcag aaatcgctgg tactcaaccc taccctgaaa tacggaaaca
     1081 ataaatttga ttacgacatt ttcaaagcca ccaaacccgg aatgaaatac cgcagcatta
     1141 tgttgcgtaa ctcaggcaat gactttgacc attcaatgat gcgcgacgga tttatgcaat
     1201 cgcttgtgat gaagcggatg aatctcgact atttagccta cgaacctgct gtttgcttta
     1261 tgaatggcgt gtactacggc atacagaacc ttcgtgaacg ctccgacgaa gattttgtat
     1321 attcaaatta cggctataac gaagatgaga ttatacttct cgaatcgtgg aaaatgaaca
     1381 ctgatccaga gtttttgaaa ctttcaaatt atataaccaa caacgacata accaaaaatt
     1441 ccgtttatga tcaggtatgt gaaatgatgg atatggataa ttttatggat tattttctga
     1501 cacaaattta tctgcgaaat accgactggc cgcacaacaa cgtgaaagcc tggaagaaaa
     1561 aagacggtgg caaatggcgc tggatactct atgacaccga tttcggctac aacctctgga
     1621 ataacgacca tactcacaat accttaacat gggcgctcgg cgagcaggca ggcacattac
     1681 ctgtagatgc tccctggtca acattactgc ttcggcgact ggtgctcaat gaaactttca
     1741 aaaaacgttt tatcgacaag tttgccattc agatgtcctc cacctttgaa gcaaaccgtt
     1801 ccaacgcgat actcgacagc ctggctgcca aaatatcaaa agagattgta tatcataaaa
     1861 acaaatgggg ctcgggacgc gactttaata ccgatatagc cacgatgaaa ggattttcgt
     1921 tcaatcgtca gaattctatg atgaacttca ttggtgcacg ttttctgaat aatccgggca
     1981 cagcagaaat tcaaatcagt tcgaataatc cgaaagcaac ctatcagtac aattcggaaa
     2041 taatacagga tgcacagatt aatttaaaac actttaaaaa tcagacatac agcctcgagg
     2101 cgaacgctgt tcccggttat accttcaaac aatgggaaat taagggaaat tcaggttcgc
     2161 aactattgat tgcaaatggc agcatctgga aatattcgga tggtaatggt gtccccgcat
     2221 caaactggtt ttcacccggg tactccgaca gcggatggaa gagcgcaagc gcagctttag
     2281 gatacggagc tttgggacaa accacaacaa ttggctatgg caccgacgcg aacaacaaat
     2341 accccacggc ttatttccgg aagaatatta atatcacaaa tctggcatcg aaagaaaatt
     2401 ttgtaatcag tactttcgtg gacgacggag ctgtcgtata tgttaacggt actgaagtgg
     2461 aacgaataaa tatgcctgct ggcgttatta ccttcaacac ctattcagct acttacaaca
     2521 atggcataac gggtaatttt accgtaccga aaaatttact taacgaaggc aataatgtaa
     2581 tagctgtgga agtacatcaa acctcagcca atagcagcga tttgattttc aatctccagt
     2641 taacctgtaa tattgccgaa gaaacgcagg ttgttttatc gccggtttat aactctgtac
     2701 tcaccactac gcggagcatc aaagccattt acgagaaaaa ttcgggcgaa gatccggata
     2761 aagacctcaa tgtggtgatt aatgaagttg tggcttccaa tacactggct gccgacgaaa
     2821 acgaagataa ggacgattac ctggaaattt acaacaatgg cagccaggcg gtgaatattg
     2881 gcggatggta cgtttcggac acgccggata atccaaccct gtatcagata ccaggcacca
     2941 atcctgcact taccacaatt ccggccaaag gaaggcttgt aatctgggct gacggagaac
     3001 cgcttcaggg cattctgcat gctgacttca aactgagcaa ggacggtgaa aaagtcacat
     3061 tgtcaaggct aaattacctg ggtgaactta cagaggtgga tgctgtcact ttcccgtaca
     3121 tggagcaaaa catgagttac tcacgggtac cggacggcag cagcacgtgg tatgtcacaa
     3181 cacctacctg taacctgacc aacggcaact ggtcagcaac ggaagatatc cgcatgaccg
     3241 cacggattta ccccacattg gttgacaacc gatttaccgt tgaaaatgcc cgcgccggat
     3301 acgtacaggt cgttgacctg acaggcaaaa tactgctgac gcaggaatgc aatactgaaa
     3361 caaccgtagt ggaagtaagc catctacaac ccggagttta catactaata gcaggaaccg
     3421 aaaaattcaa attcattaaa aagtaattca gcaagcgcgg caagctcatt aaaatgaaaa
     3481 aatccatttt cccaaaaggg atatggattt tttcataaat cccaaccata ttataaaaaa
     3541 acaatcatcc cataaggata tgctgcgcaa agcccgtttt tcggacaaaa gaccctgaca
     3601 aatagccgaa acttagagaa agtaaaaaaa gaagaccctt aaacaaacgt ttaaaggcta
     3661 aaaacaaatg aaatgaacca ataaataaat atttaaaggt cgaaaacaaa tgaaatgaac
     3721 cgataaacaa atgtttaaag gtagaaatca gatgatttta accaataaac aaacgtttaa
     3781 aggcagaaag catatgaatt gaaccgatga ctaaatagtc aacgatagaa atcatatgaa
     3841 ccgatgtgta aatatttacg gagggggaat gtgctgaagt ataatttctt tcctaacttt
     3901 ttcgtttcag caccaacatc tgacactttc ggcagtcgaa ttccagctga aactgctgat
     3961 ccttgtacga aatgtaaagt ggttctttga atgtagcttt atatccttct ttcggacacc
     4021 agcccaggga gtatttgatg caatgtctgc aaaacataag cggcacaccg ggttcggcct
     4081 ggacttcaaa tccgggcata acctcttcca ctccatgctg caaataaaat tcacgagccc
     4141 gttcattggt aacattgccc agataggtca gtttcttttc aggaaatgca gcctcactgt
     4201 tggccggcct tcttttccct gtcacgtaag cttcacggcg tttttcgtcg agcaattcta
     4261 ttgcctgtcg ccgccattca ttgagtaccg aagccggtaa aaaccagggt gaattaaccg
     4321 aaatatcaac attacgagcc atataaatgg tatttccgag tttcgtcagc tgactttcga
     4381 tggtttgtat ggcacgttca gcattattgg cagcttgctt ttcggataca aactcactaa
     4441 tcgccgacaa gccttcctca tcggtaattt gtatcgagaa accatccggt ttttccgaaa
     4501 acaggatatt gacgccgatt tttctctcgg aggttttacc ggcaagtatt ttatcaaatt
     4561 cctgatcgtg atttcgatac agaaatgtgt ttcggtccat ttcaggcatt tcggcgggaa
     4621 agactttccg tccttccacc ctgtttactc tgaaaccttt cagaattccg tcacgggtga
     4681 ggaaacacaa tccgtcgccg ttgtgaattt ccttttctgt ttttatttcg ataaactgac
     4741 cggaaatata tgtcactgtt cctatttcct ctccgattga cttgggtgtt tcgggctggc
     4801 tgatattctg ttgtcgtccg ttaaggaaat aatcggtggc actccttctg aaacttttat
     4861 ccggattggg ttcaaaaaag aatgttgttg ttcccgagga agattttctg acatcttttc
     4921 tacattccag aattgcatcc agtttctttc ggtaaaaagc cgtaatattt tttacataat
     4981 ccgcatcctt gagtctgcct tcaattttga atgaacttat accggcatca attaattcat
     5041 ccaggtggtc ggacaaatca aggtctttca gcgaaagcaa atgtttgttt ttcaccagca
     5101 cctgtccgtc actgtctgta agttggtagg gcaagcggca gtattgggaa cattcaccgc
     5161 gattggcgct gcgtcctgtc atagcctgac tgatgtagca ctgtccgctg taactcacac
     5221 aaagcgctcc atgtataaag cactccagtt ccacattggt attatcgtga atttccctga
     5281 tttgacggag tgttagttcg cgggccagca ctactctcga aaaacccaca tcctgaagaa
     5341 atcggacctt ttccacagtc cggttgtcgg tctgtgtgct ggcatgaagt tctatcggag
     5401 gcaaatccag ctgtagaata cccatatctt gcacaatcag ggcatccgtc cctatttcgt
     5461 acaactgatg tattagtttt tctgcttccg tgagctgtct gtcggtcagg acagtattca
     5521 atgcaaccag aaccttactg cggtaacggt gtgcatagga aataagctgt tcgatatcag
     5581 caatgttatt tccggcagca gcccgtgcgc tgaacatcgg cgcaccgata taaacagcgt
     5641 ctgcgccgtg attgatggca gccagaccgc actccaggtt tttggcaggt gagaggagtt
     5701 ctatatttcg catgctcttg ttactggata ttagccaaat cgtatggtgt ctcctggtac
     5761 acaaaatagt tcagccagtt ggtgaagaga agattagcgt gacttcgcca gagcactaca
     5821 ggttcgttag ccgggttgtt ttcacgatag taatttacag gcattttaat gggtaagcct
     5881 tttttcacat cgcgtttata ttcggtatcg agtgtacttg ccgaatattc cgaatgtccg
     5941 gtaatgaaaa aatcacgtcc attacgtgcc ataaccatat acacaccgga ttcctccgat
     6001 tccgagagta atgtaagttc gggatttttc agaatatctt ccctccggat ttcggtatgt
     6061 cggctgtgag gaacataaaa cacatcgtcg aagcccctga agatcggatt aagcttatcg
     6121 taaatcttat gttcaaaaac tccgaacatt ttctcgggga gtggatattt cgggatatta
     6181 tagaaatgat gtagtccggc ctgagccgcc cagcaaatat acagggttga agtcacatga
     6241 taatgtgccc agtcgaaaat atcggttacc tccttccagt atttcacttc ttcgtattcc
     6301 agttgttcca caggagctcc ggtgataatc atgccgtcgt agttgctttt tctgatatcg
     6361 tcaaaatttt tgtaaaacgc catcatatgc tcgatgggtg tgtttttcga agtatgactt
     6421 ttcagtcgca aaaagtcaat ctcaatttgc aaaggtgaat tggatagcag acggatcaaa
     6481 tcggtttctg tggttatttt cagtggcatc agattcaaaa taacaatttt gagaggtctg
     6541 atctcctgac tttgcgcccg ttcggtatcc attacaaaga tattttcttt gcgcaaagca
     6601 tcgaccgccg gaagtgtatt tgggatattt agtggcatat aaacagttta tagtttataa
     6661 attgctgaat tatgatcaat agtattttcg gtaaattttc caataatcgt ttgacatgag
     6721 aaagtcgttt aagaagatat tcagcgaatc gcgtaactga cctgcagatg ggtttacacc
     6781 ccatgaaaac tgctgagtaa aaccaaccgg catcgagata tcaatgttag taaaaattcg
     6841 tttcagcctt tttgcctgca gttcatcaca gatggtatac tttattttcc cggccgagac
     6901 catttgcacc agatcttccg ttgatttatc aggaatttcc acgattttta tcgaatcggc
     6961 aatttcattc gacaaatttt cgagccgctg cctgaatggc gaacggacag gaatgcaaat
     7021 actatcttcg gtcagcaaat gatggttacg aatggctttc acgttggtag tatcaataat
     7081 ttcctgctgc acaagcattt gccgtcctga aattattggt attgaaactg ccagtttctt
     7141 ttttatttcc gaagtattcg gtaagttcat ggctatcagg tcatacctgt tttctaaaat
     7201 tcctgcaatt gctgagtcta tgttgttctc cggattgatt tccagttcaa gacccagcga
     7261 atcggcaaat gccttcacca gttcatattg aaaaccttca atctgatcgt tgtaaagttt
     7321 aaatccgagc gaactttttt cagtaactac ccgcaagcgt cccgattggc gtatctcaga
     7381 caaatcgcgt tgttcgttga aaatatgacc tttacgcgac atattgagcg tgagagtcat
     7441 cacgagtatc atacccgcta ccattcctaa gataattttc gcacagcgat tcattcgacc
     7501 ttctgatgaa gtttaattat agaaattcca cgacaaacca agttttagca ccggaggatt
     7561 tatagcataa ttgggcatat agaaatgtgc tccgctcata aagtatttgt tcaggtgatt
     7621 atattcaata aaaaatctgg attgtttcag atgaaagttt gcatatacat tcaggattgg
     7681 ataattaccg ataagtactt ctttctgtgt ataaaactgt ccggttgcag gcatgtatgc
     7741 cggagcgtaa tagttggtat gatagcgcac attggctcca atctgaggat ataaatcttt
     7801 aaaccatttg gaataatagt agaaattgtg aaacaaggta agcgttggca gtggaagtac
     7861 atcgggtacc gaactaacct gataaacagc gttgttttca agtacaaatt ttccgaaatg
     7921 aaaatcctgt ttcaaatcgg ccgaaagcac ctgaacatta ccattatact gtgtaggcaa
     7981 cgcgttttcg ttgaaataaa tataatcggt tatattctca acacctacat tgagcaacgt
     8041 ataaagtttg ggcagcgaga atgaaccgct gatatgcgtt ttgtacattt tactgaactc
     8101 attatcccat ttgaaatgcg tggaataata atgctgtaga aaatatgccg gttcttcatt
     8161 ccggacaaat gcattggcgg taagctggat attctgattg aacaggttaa aatttccggt
     8221 agccttacct tctatccgga attcgcccag tttatagccg atgaggtaaa tatcgcccaa
     8281 tacgttatac cgaaatctgg ttccctgatt tttcgataat atccccccta cacgcgtgtt
     8341 cgattttgtt gtccacgcca gtgtggtatc gggttcgtat gtaaattgct gtatctcatt
     8401 ttctacgtat cccgtcattc cgaatttcat ccatttgtta aattcttctt caagacttat
     8461 tgaaaacagg ttggtcagcg tacgtacagc agccgtatcg ttcgcatctt tttttacagg
     8521 atctgtaaat ttgtaaaaat ctgttaccag tgtaggctca taatatcgct ttttcatttc
     8581 ctcgagcttt atcatgtgtc caaaacgggt tactggcaca taatcgtaac gtacagaatc
     8641 tttgttcact ttcacttcgc ggttgaaacc tatgctgtaa ctgtgattat aaaaagcctg
     8701 atttttgcga aaaactgaat atcctttcag aaaaacaggc atatcttgcg gtttcaccga
     8761 aatatcggga ttagtgaggt aactggggtc gctcaggccg ccgctttcat aattgaaatg
     8821 attgttgacg gctatcagac cattacccga ataatgtttg ccgttgtacc gaccaaaaat
     8881 gcctcccgag aatcttttta ccgcctgatt atcatattcg cccacagcat gtatataatc
     8941 cagagtagta ccgaaatttg cctttttcga tggacttgcc gtaaacagga acttcacttg
     9001 ttcttctttc cggtaggcag tacctccggt aagataagtc agattggtgt agggataagt
     9061 agcatcgtaa aacaatgctg aagagatatc cagagtgtag ggtttgtatg cttcgtcaaa
     9121 taaaaaatcg gagccctgag gtcttttaaa gtacagtttc gactgaagcg gcgagcccag
     9181 gttgcccgag aaggaattgg caatgctgaa tgattccact ggattttgat ccgggaaatt
     9241 gatagtggca gtatccatag gaacagtatc ggcaacagcc atttgttcag atatcgtcca
     9301 ggatttgata tgttttaccg gttttgcagg tgtttttttc tgtgaactga ttgaatctgt
     9361 ctgcgggaaa gcttgcatcc ccaaaataac cagactataa ataagtatgt atcgtttcat
     9421 caataagtgg tttagcacga ataaatcgca aagatacaaa aatttataaa gcttaaattc
     9481 aatgcgataa ttataaaagg gaaaattgaa aaattataac gattatttga aaaatgattt
     9541 tattttccac ttaaaacata actgaacctc attcagttat gaatccccca aaaaacgggc
     9601 gtttaacttt ataaatcgta caaaaaaaaa acaaggatgc tattgtcggt caaataatta
     9661 tttgtatctt tgcaccctca taaacgaaat tatttacaaa ccattaaaaa ggtaaaaaac
     9721 agtatgttaa atcattatga agccgttttc attttaactc ccgttttgtc tgatgttcag
     9781 atgaaggaag cggtagataa gttcaaagct gtcatcacag acaacggagg aactatcaca
     9841 aatgaagaaa actggggcat gcgcaagctg gcttacccta ttcaaaaaaa atcgacagga
     9901 ttttatgcct tgttgcaatt cgatgttgaa cctacagtaa tcgctacact tgaaactcag
     9961 ttccgccgcg atgaaaaagt acttcgtttc ttaacattcc gactcgataa gtatgcttac
    10021 gagtacgctg aaaaaagaaa aaacgtgaaa tcacaaccga aagaagtgaa agaagaagaa
    10081 gcaaaaaaag aaactaaaga agaaaaggag gattaaaaca tggcagcaca ctcaggagaa
    10141 atcagatatt taactccacc tacagttgat gttaaaaaga aaaaatattg ccgtttcaaa
    10201 aaaagcggta ttaagtacat tgattacaaa gatccggaat ttctgaaaaa attcctcaac
    10261 gaacaaggaa aaatacttcc ccgtcgtctt accggtactt cgctcaaata tcagcgtaaa
    10321 gtagctcagg cagtaaaaag agcacgccac ctggcattgc ttccttatgt aaccgatatg
    10381 atgaaataat agaaaggaga acaaagtatg gaaattatat tgaaagaaga tgtaatcaac
    10441 ctgggttaca aaggtgatat cgtgaaggtt aaagacggct acggtcgcaa cttcctgatc
    10501 cctaccaaaa aagccgtatt agctacagaa tcagctaaaa agatgctggc agaagacaac
    10561 agacaacgtg ctcacaaact ggaacaaatc aaaaataccg cactggaact ggctgaaaaa
    10621 ctgaaagaag taagtctgac catcggagca aaaacaagct caaccggtaa gatatttggt
    10681 gcagttggtc caatccaggt ttctgaaagc ctcgaaaaag caggtcttac tatcgacaga
    10741 aaaattatca gtatcaaaga accagttaaa gaaattggtt cctatcaggc tactatcaaa
    10801 cttcacaaag aagtatctgt agaaattcca ttcgaagtgg tatctgaata agaattctct
    10861 tcgattcaaa atcatcttaa aaaccgattt gttgcaaaat gacaagtcgg tttttttatt
    10921 taaatttctc atacccgact ttccacaata cagggctcaa atcagatttc aagttgtcaa
    10981 tcaaacaatt gttatctttt ttctgagttt cttacaactt taataaatat tccgtcacaa
    11041 tttcatcaag tttttcaaga aaatttttct tcattttttt tgtatttcta ttctcgtaat
    11101 caaaatattt atattttctt tgcgattaga aataaacact tttttttgat taaggatgtg
    11161 tggttcatta taaataattg tcacattact gatgttgaag gcttattttt aatcaggttt
    11221 caacaaatta gacgtgttgt ttgttattta tctaattgcc aggttttttc aaaaaataag
    11281 tatttttttt tctaatacaa aaatcattat ttctcaatta ttaactttaa atttagtcag
    11341 aatgaaaaga aaaaatcttt ttgaactgag agttgtaatt gcactaatct tcagtgtact
    11401 cacaattaca actttcgcac aaacaatcac tgtcaccggt accgtgtctg acacaaacag
    11461 cgaaccagt
//
LOCUS       DAOPYB010000095        11459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6440_length_11459_cov_9.792792, whole genome shotgun sequence.
ACCESSION   DAOPYB010000095 DAOPYB010000000
VERSION     DAOPYB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11459
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6440_length_11459_cov_9.792792"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PKH58_08190"
     CDS             <1..611
                     /locus_tag="PKH58_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX89045.1"
                     /translation="FGGLDHVIASGKNVNILVLDTEVYSNTGGQSSKSTPVGAVAKFA
                     ASGKKIRKKDLGMIAATYGYVYVAQVAMGANQAQTLKALREAESYDGPSIVIAYSPCI
                     SHGLKSGMGKAQSEQKRAVECGYWHLYRFDPRLEAEGKNPMTLDSKEPDWTKFQEFLQ
                     GEVRYTSLMKQFSAEAAELFKMAEENAKWRYKGYKRMAEQQF"
     gene            899..1699
                     /locus_tag="PKH58_08195"
     CDS             899..1699
                     /locus_tag="PKH58_08195"
                     /inference="COORDINATES: protein motif:HMM:NF019515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1670 domain-containing protein"
                     /protein_id="HNX89046.1"
                     /translation="MINNSSIHKWDRLRQKQLDTQFINKLQAGMNCSMFEAKAILNAV
                     YEVYQPFFDNSASLKPGQICFEVVGVENSPKEKLSDCQMRTVILTLDAGEEDLQIRQE
                     KGVIELRQHRLQRICNEALQQDGLLTVEDLANRLLNCGERTITRDIHALKQQGIILPL
                     RSTIKDMGRAVTHRELIVRYWLSGMEYSDISFRTNHSIDAIANYVDKFKRVISLAKDN
                     YEIHTIAFFVKISKALAQQYYDLYKSMDIVPHRKKELDELLKKTTVQP"
     gene            1714..2469
                     /locus_tag="PKH58_08200"
     CDS             1714..2469
                     /locus_tag="PKH58_08200"
                     /inference="COORDINATES: protein motif:HMM:NF019515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1670 domain-containing protein"
                     /protein_id="HNX89047.1"
                     /translation="MIRKPDAVRKYHAAHDRFLKPAIINFFARKFAGHFGPIVRENIA
                     DALIDIFEQNVPQTNRIKHGQILWNALDKTTRADTSNRKYVPVILTLVTDQEINLFEK
                     NKSIREIRKQVIARIIRQAYEQGGILSTRDISLILSTNDSLISKRRIEYEEENKTVLP
                     HTGVLHDMGSTITHKKQIVYKHIIEKKSTNIVARETNHSQKAVDHYIRDYYRVKTLVD
                     DNKDIDFIHIATKIAKPVISQYQDIINKYVKDH"
     gene            2836..3504
                     /locus_tag="PKH58_08205"
     CDS             2836..3504
                     /locus_tag="PKH58_08205"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNX89048.1"
                     /translation="MRTKITALLLLTLFIAPLPAQKKVLFGGKDLTNWYAYNAVNLKN
                     ERADEVFPVEKHLIRLRGKNPGYLMSNQSFTNFRLTALFRWNPDKDIVKLSKTRNSGL
                     MYLVPDTAKDMLWCTGIQYQIKEKSTGDFIFLGGVTAEIDGKKTTAGKSMVYPKTQEA
                     EKLVGKWNKLEITVRNGTIVQKLNGKVVNTAKNPSVTSGRVLLQYEGYPVDFKKIIVQ
                     ELKW"
     gene            complement(3771..7292)
                     /locus_tag="PKH58_08210"
     CDS             complement(3771..7292)
                     /locus_tag="PKH58_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HNX89049.1"
                     /translation="MENIKSNEVIEWAKQFLDKAEKEISPQELKEQNKYAILVQNPND
                     KVLLSKMLDESSQIHDSKKLARRMKILLKRYGVPEFFNSSDHFMLSLFTSFGYWFDFI
                     AVPIFKKRLRSETSAIIINERPDLLTEHLEGRRSQNIGQNVNLLGEVVLGDGEARHRY
                     EHYLEALKDPKINYISVKLSGIYAQINALNYDQCKNELCELMGNIYQHAIDYPFIDED
                     GVPSSKFVNLDMEEYKDTEQTLDVFITTLSEPRFKDLTAGIVVQAYLPDAENFQAELL
                     EFARKRVAEGGAPLKMRLVKGANLQMESIVSSLRGWENPVYDSKVKVDANYLHILDKA
                     MLPENASALHLGVASHNFFTIGYAYLLAKKNGVEDYVTFEMLEGMANHLPRVMRSLQK
                     QIILYTPVVKDEHFLNAVSYLVRRMDENTGKDNFLTYSFNLKFGSKQWNFLQEQFLEA
                     YAIKDTIDTTPKRKQNRNLPQEPAVNITEFTGEPDTDFDLKPNRLWADEIRKKWQKTA
                     TDKPYEIPAQIGDKEVMNEKKYRYYDRSLEEKICVCEVSQASADQVKEILAIAEKDGS
                     EWRKTTLDQRNIILHKVADNLGKSRGDLIGCMSAITGKTFPEGDVEVSEAIDFCRFYP
                     YSMRTFENISTLNYAPKGIVLVIPPWNFPTAIPVGGVAAALAGGNTVILKPATVAFPV
                     AWQFAQCFWDAGVPKDALQVVCPDSREALSHLTSHPAVKHIIMTGGTDTAFRILENNP
                     SCPLSAETGGKNAIILTASGDRDHAIQNVVKSAFGNAGQKCSACSLLLLEKSVYDDPS
                     FKSKLIDAVTSIRTGSAWNGTNIVGPMVDNKNEKLLHAVNNLEDGEFWLVKPEFVDKD
                     GYILKPCVKWGVKPDSYTFRTELFGPLLAVVCIDNLQHGIDLMNKKTDFGLTSGLQSL
                     DENERRLWKNSIEAGNLYINRGITGAIVQRQPFGGMKRSAFGGGIKAGSVNYVSSFVN
                     FTENSKILQLNPAKASFETLKGQLTGDEYNRLCYASENYQQAWTNEFSQEKVTQQLMG
                     ELNTFRYLPLKNMIFRVLPGDNNADMLMVVLAAHTACTPLTVSISDSDEKMKLLLALP
                     VKDITIVKQSDEEFVAAMDKYDRVRCCSAALPDSYYQKAAKLGKYIAVTSPLVEGRVE
                     LLHYLKEQSISYEYHRYGSFTEEDAAI"
     gene            complement(7551..8849)
                     /locus_tag="PKH58_08215"
     CDS             complement(7551..8849)
                     /locus_tag="PKH58_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX89050.1"
                     /translation="MISKELIERLFTEFGTDDINQLSIREVGKLVSMIEKESGEEFIH
                     MEMGVPGLSASQIGIDAEKEALERGVGAAYPNIEGIPVAKEEIARFAKLFMNLDTPPA
                     HCIPTVGGMQGSFAALLAAARSNPDKPCTLFIDPGFPVQKSQLHLMGVPFKSFDIFQF
                     RGDKLRAKLEEYLKQGDICSICYSSPNNPSWICFTEEELQVIGELATRYDVIVIEDLA
                     YFGMDFRQNYGLPGESPYQPTVAHYTDNYILLLSASKIFSYAGQRIGMMIFSEQTYTR
                     RYPNLKNYFTSDTLGHFIPYGVLYATTAGTAHTAQYALAAMLRAANDGRVNFVENLHL
                     YGQKARLMKEAFLKHGFYIVYNDDTRELADGFYFTIAYPGLTSGQLMNKFLHYGISAI
                     SLAITGSDNPNGLRACVSFVRLDQIPVLNERLTRFVQDMK"
     gene            complement(8874..9461)
                     /locus_tag="PKH58_08220"
     CDS             complement(8874..9461)
                     /locus_tag="PKH58_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89051.1"
                     /translation="MKVWLKEIAFVLLSVSVFSGCSIITNGINYKRDKLMDRTRYTLQ
                     QSFITNERHEQDYQQQISLLKEIDENGQSTYTMYDIISIPLKSFDISNEMYIIIDKEP
                     IPLPLTLKERLYDNDLKNGINFPEQSTDTAQNFSISSTKVTRMIHPLDDELIEKISRA
                     KAVYLRYYVGPEMITSEIKGTKLYSLKKLISRKYK"
     gene            complement(9486..10787)
                     /locus_tag="PKH58_08225"
     CDS             complement(9486..10787)
                     /locus_tag="PKH58_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNX89052.1"
                     /translation="MIWNKEIECMSRDAMRKLQDERLRELVARVYNNVPFYHRKMEEA
                     GVKPSDIHGIDDIAKLPFTYKQDLRDNYPFGLFAVPMKDIVRVHGSSGTTGKPTTVGY
                     TKNDIQTWKEVVARCFTMSGIDSHDIVQVSYGYGLFTGGLGAHYGAEEIGCSVIPISG
                     GNTRRQLQLMADFGSTVLACTPSYALHLADAMSEFGYSLSDMKLRVGVFGAEPWTVNM
                     RKELERKWNITAHDIYGLSEIMGPGVANDCIYHNGLHIHEDHFFPEIVDPKTKEPVPY
                     GQEGELVFTTLTKEGIPLIRYNTRDLSSLYYDKCECGRTTVRMEKITGRSDDMLIIRG
                     VNLFPSQIEHVLLDLQETSAHYILYVDRKNNLDTLELQVELDENKLTDTIRDLQNLSR
                     KIEHALNSAIGLSVKVTLVEPKTIARSEGKATRVVDRRTKE"
     gene            complement(10895..11314)
                     /locus_tag="PKH58_08230"
     CDS             complement(10895..11314)
                     /locus_tag="PKH58_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNX89053.1"
                     /translation="MTIRQLSVFMENKPGYLNEVLAVLARNNININALTIADTSDYGI
                     ARILVSDPQLALDKLHEENFAVRIHDVLSLEMGAAPGSLNNILNLFATANISIEYVYA
                     FSYGSKSIVVLRTDNREKAVEVIRDNKLKSITEDDLK"
BASE COUNT         3129 a         2498 c         2565 g         3267 t
ORIGIN      
        1 gtttcggtgg tctcgaccac gtgattgctt cgggtaaaaa tgtgaatatc ctcgtactcg
       61 atactgaagt ttactccaac accggcggtc agtcgtccaa atccacacct gtaggcgcgg
      121 ttgccaagtt tgctgcttcc ggaaagaaaa tccgcaagaa agaccttggt atgattgctg
      181 ccacttacgg ttatgtatat gtagcacagg ttgcaatggg agccaatcag gcacagacac
      241 tcaaagcttt acgggaagcc gaatcatacg acggaccatc catcgtaatt gcctactcac
      301 cctgtatcag tcacggactg aaatccggaa tgggcaaggc acagagcgaa cagaaacgcg
      361 cagtggaatg tggttactgg catctgtacc gtttcgaccc gagactggaa gctgaaggca
      421 agaacccgat gaccctcgac tcgaaagaac cggattggac taagttccag gaattccttc
      481 agggagaagt tcgttacact tcgctgatga aacaattctc ggctgaagct gccgaactct
      541 tcaagatggc cgaagaaaat gccaaatggc gttacaaagg ttacaaacgt atggctgaac
      601 aacagttcta aacgtaatct gcaatataat caagaggctg tcccaacatc ggggcagcct
      661 ttttttaagc ctcaccccat aaacatttca gctattttgt ggcttatctc acccctctcc
      721 tatatttgtg ccatatgaca ttgaatagga ttaaaagaaa aacgttcttt gaaattttgt
      781 tataatagat gatatttaga actttgcagt agccgcatga agtgaaggcg aagcgtagcg
      841 aagccgaaac ggaatgcggc tactgcaaag acaggtttat tgcttaatac ttacgattat
      901 gataaacaat tcatctatcc acaaatggga tcggcttaga caaaagcaat tggacactca
      961 atttatcaac aaacttcaag caggcatgaa ctgctccatg tttgaagcaa aagctatttt
     1021 gaatgcagta tatgaagtgt accaaccttt ttttgataac agtgccagct taaaacccgg
     1081 acaaatttgc ttcgaagtgg ttggagttga aaactccccc aaagaaaagc tatccgattg
     1141 tcaaatgaga actgttatcc taacacttga tgcaggagaa gaagatcttc aaatacgtca
     1201 ggaaaaaggg gtgattgaac ttcgccaaca tcgccttcaa cgtatctgca atgaagccct
     1261 tcaacaggac gggttgctaa cggtagaaga ccttgccaat cgattgctta actgtggcga
     1321 gagaaccatt acacgcgata tccatgcgtt aaagcagcaa ggaattatat tgcctttacg
     1381 atccacgatc aaggatatgg gacgagccgt aacgcatcgc gaactgattg tcaggtattg
     1441 gttatcaggc atggaatact ccgatatcag cttccgcacc aatcacagta tcgatgccat
     1501 tgccaactat gtggataaat ttaaacgggt catttccctg gctaaagaca attacgaaat
     1561 ccataccatc gctttctttg tgaagatttc aaaagcactg gctcaacagt attatgacct
     1621 gtataaatcc atggatattg tacctcatcg taagaaggaa ctggatgaac tgctaaaaaa
     1681 aacgactgtt caaccctgaa aaattctttc aacatgattc gtaaacctga tgcagtcaga
     1741 aagtatcatg cagcgcatga ccgctttcta aaacctgcca ttatcaattt ctttgcacgt
     1801 aagtttgccg gacattttgg cccgattgta agagaaaaca ttgccgatgc actcatagat
     1861 atttttgaac aaaacgttcc tcaaacaaac cgcattaaac acggtcagat actatggaat
     1921 gctttggata aaaccactag agccgacact tctaatcgaa agtatgtgcc cgttattctc
     1981 acgttagtaa ctgatcaaga aataaatctt tttgaaaaga ataagtctat ccgagaaata
     2041 cgcaaacaag taatcgccag aattattcgg caggcgtatg aacagggtgg aattctttca
     2101 accagagata ttagtctgat tctatcaact aatgattctt taatatcaaa aagaagaatt
     2161 gaatatgaag aggaaaataa aacagttctt cctcataccg gagttctcca cgatatgggc
     2221 tctacaatta cacataaaaa acaaattgtt tataaacaca ttatagagaa aaaatcaaca
     2281 aacattgtgg cccgtgaaac aaatcactca caaaaggctg ttgatcatta tatcagggat
     2341 tattaccggg taaaaactct tgtagatgac aataaagaca ttgatttcat ccatattgca
     2401 accaaaattg caaaacctgt aatctcacaa tatcaagata ttataaacaa atatgtcaaa
     2461 gatcattaat tattaaagtg actttgtgaa aagtcaaatg acattatcaa aggagaggga
     2521 gccggaggga ggcctgactg ctgaaagccc cctaaatccc ccttggggga tttcagcaac
     2581 atcggttgta taggaatata ctcaaattgt aaacctgttt caggacaaga cagatgaaca
     2641 aagcagggct ttctatctta aaagttttca gttgtgggta taatactgta attttacagc
     2701 gggagaacaa acttccgctg tatttatata tcaattatga gaaacagttg tttgagaaaa
     2761 actcaaacac gggaaaatga ttaataacca gcctgtagcc cccttatagg gattaagggc
     2821 atatatttct atcacatgag aacaaaaata accgcattat tactgctgac attatttata
     2881 gcaccccttc cggcacagaa gaaagtgctt ttcggaggga aagatctgac gaactggtat
     2941 gcttacaatg ccgtgaactt aaaaaacgaa cgcgctgatg aagtttttcc ggtcgaaaag
     3001 catcttatcc gtttacgcgg gaaaaacccg ggctatctga tgagtaatca gtcctttacc
     3061 aacttcagac tgactgccct gtttcgctgg aacccggaca aagacatcgt aaagctatcg
     3121 aaaacacgca acagcggact gatgtatctg gtgcctgata ccgcgaagga tatgctttgg
     3181 tgtactggaa ttcaatatca gataaaagaa aaaagcaccg gcgatttcat ctttttaggg
     3241 ggtgttacag ccgaaattga cggtaagaaa accacggcgg gtaaaagtat ggtatatcca
     3301 aaaacacagg aagcagaaaa actggtcggt aaatggaaca aactcgaaat tacagtccgg
     3361 aacggaacca tagttcagaa actgaacgga aaagttgtca atacggctaa aaatccgtcc
     3421 gtaacatccg gcagggtgtt gctgcaatac gaaggttatc ccgttgattt caaaaagatt
     3481 attgtacaag aattaaagtg gtagaacaaa cttatcgtgg caagatttct tatttttttg
     3541 tcgtgaaatg acggtgaaat ttatgcgttt ttgtcgtaga ataacggtat atctgtattc
     3601 tttttcttaa aacagggctt aaagcccgtg tcttccctgt atcgtactga aattaagagc
     3661 agctgactgt tatgtagaaa gccaaaaagg actaagaagg gttattacac tcctgcttag
     3721 tccttttcta ttcattcacc ggaaactccg gtttgatgtc agtttatcaa tcaaattgca
     3781 gcgtcttctt cggtaaagct gccgtatcgg tggtattcgt acgaaatgct ttgttccttc
     3841 aggtagtgaa gcaactcaac ccgtccttcc accagcggtg aggttactgc tatgtattta
     3901 cccagtttgg cagctttctg ataatagctg tcgggtaaag cggctgagca gcagcgaaca
     3961 cggtcgtatt tatccatcgc ggctacaaac tcctcatccg actgttttac tatcgtaata
     4021 tccttcaccg gtaatgccag aagcagtttc atcttttcat ccgaatcgct gatgctgaca
     4081 gtaagcggtg tgcaggctgt atgagcggca agaaccacca tcagcatatc ggcattgtta
     4141 tcgccgggca acacgcgaaa aatcatattt ttcagcggaa gatagcggaa ggtgttgagt
     4201 tcgcccatca actgctgggt tactttttcc tgcgagaatt cgttcgtcca cgcttgctga
     4261 taattttcgg aagcatagca gaggcggttg tattcatcgc cggtcagttg tcctttcagt
     4321 gtttcaaaag aggcttttgc cggatttaac tgaagtatct tgctgttttc ggtgaagtta
     4381 acgaaactcg acacataatt gaccgagccg gctttgatgc cgccaccaaa ggcggagcgt
     4441 ttcattccac cgaaaggctg acgctgtacg atggctccgg tgatgccacg gttgatatac
     4501 agattaccgg cttcgattga atttttccac aatcggcgtt cgttttcgtc gagactttgc
     4561 agcccggatg taagaccgaa atcagtcttt ttgttcatca gatcgatacc atgctgcagg
     4621 ttgtcgatac ataccactgc cagcagcggt ccgaaaagtt ctgtacggaa agtatagcta
     4681 tcgggtttta cgccccattt tacacagggt ttgaggatat aaccatcctt atccacaaat
     4741 tcgggtttca ccagccagaa ctcaccatct tcgaggttat tgacagcatg aagcaacttt
     4801 tcgtttttat tgtccaccat cggacccacg atgttggttc cgttccaggc gctgccggta
     4861 cggatgctgg tcaccgcatc aatcagtttc gatttaaagg acggatcatc gtacacactc
     4921 ttttcgagca ggagcagcga acatgcggaa catttctgtc cggcattgcc gaatgctgat
     4981 ttcaccacat tctgtatggc atggtcgcgg tcgccgctgg ctgtgagtat aatggcattc
     5041 tttccgcctg tttctgccga aagcggacaa gacggattgt tttcgaggat gcggaaagcg
     5101 gtatcggtgc ctccggtcat gatgatgtgt ttcactgccg gatgcgaagt caggtgactg
     5161 agcgcttcgc ggctgtcggg gcataccacc tgcagggcat ccttcggcac accggcgtcc
     5221 cagaaacact gagcaaactg ccaggcaacg ggaaatgcta ctgttgctgg tttcaggata
     5281 acggtgttgc caccggcgag cgcggcagcc acaccaccta cgggaatagc tgtcgggaaa
     5341 ttccagggcg gaataacgag tacaattcct ttgggtgcat agttcagcgt gctgatattt
     5401 tcgaatgtac gcatcgaata cggatagaaa cggcagaagt cgatagcttc cgaaacttcc
     5461 acatcacctt cggggaatgt ctttccggtt attgccgaca tacatccgat aaggtcgccg
     5521 cggctttttc cgagattatc ggccactttg tgcaggatga tattacgctg gtcgagggtg
     5581 gtcttgcgcc attccgaacc atccttttcg gctattgcca agatttcctt cacctgatca
     5641 gcagatgcct gactgacttc gcaaacacat attttttctt ccagactgcg gtcgtaataa
     5701 cggtatttct tttcgttcat cacttcctta tcgccaatct gagccggtat ctcgtaaggt
     5761 ttatcggtgg ctgttttctg ccatttctta cgtatttcgt cagcccaaag tctgtttggc
     5821 ttcaaatcga agtcggtatc gggttcaccg gtaaactcag taatattaac ggcaggttcc
     5881 tgaggcagat ttctgttttg tttccgtttt ggagtggtat caatggtatc ctttatggca
     5941 taagcttcga ggaactgttc ctggaggaaa ttccattgct tgctgccaaa tttcaggtta
     6001 aacgaatagg tcaggaagtt gtccttaccg gtattttcat ccatacggcg caccagatac
     6061 gaaacggcat tcaggaaatg ttcgtcttta accaccggtg tgtaaagaat gatctgtttc
     6121 tgcagactgc gcatcacacg cgggaggtga ttggccattc cttcaagcat ttcgaaggta
     6181 acataatctt caactccgtt tttcttagcc aggagataag cataaccgat ggtgaagaaa
     6241 ttgtgcgagg caacacccag atgcagggca ctggcatttt cgggcaacat ggctttgtcg
     6301 aggatatgca ggtagttggc atccactttc accttggagt cgtaaaccgg gttttcccat
     6361 ccgcgcagcg atgaaacgat gctttccatc tgcaggttag cgcctttcac cagtcgcatt
     6421 ttgagcggtg ctccgccttc ggccacacgt ttgcgcgcaa attcaagtaa ttctgcctgg
     6481 aagttttcag cgtcgggaag gtatgcctgc actacaatac cggcggtaag gtctttgaag
     6541 cggggctcgg aaagagtggt gataaaaaca tcgagtgtct gttcggtgtc cttatattct
     6601 tccatatcca ggtttacaaa cttggaagag ggtactccgt cttcgtcgat gaaaggataa
     6661 tcgatggcat gctgatagat attccccatc agttcgcaga gttcattttt acactgatca
     6721 taattcaggg catttatctg cgcgtagata cccgagagtt tgacagaaat atagttgatt
     6781 ttcggatctt tcagcgcttc cagataatgt tcgtagcgat gccgtgcttc cccgtcgccg
     6841 agtacaactt caccaagcag gtttacattc tgcccgatat tctggctacg tcggccttcc
     6901 aggtgctcgg tgagcaggtc ggggcgttcg tttatgatga tggctgatgt ttcggaacgg
     6961 agtcttttct tgaaaatagg aacggcaata aaatcgaacc aatatccgaa agaagtaaat
     7021 aaactcagca taaaatgatc ggatgaattg aagaattcgg gtactccgta acgtttgagc
     7081 aggattttca tcctcctggc gagtttctta ctgtcgtgaa tttgtgaact ttcatccaac
     7141 atcttggata aaaggacttt gtcgttgggg ttttgaacca gaatggcgta tttgttctgt
     7201 tcttttaatt cctgtgggct gatttccttt tctgctttat cgagaaactg ttttgcccat
     7261 tcgatgactt catttgattt aatgttttcc atagattttt tgtcctgtct gagcaggctt
     7321 tttgagtttt atatcagatt tttgttaatt attattgatt ttgtaaaaat acaaaaaatg
     7381 ttgaaacaga acattcactg acaggatatt tattttacat ttttttattg attttatgaa
     7441 tggaagttaa tctgtgtagt tccggattag tccggcattt gagtacaaaa aaaacaggta
     7501 ctaaagcgat atttcgtctt agtacctgta attaactgta aaatatatgt ttatttcata
     7561 tcctgtacaa atcttgtcag cctttcattg agtacgggga tctgatccag tctgacaaac
     7621 gaaacgcagg ctctcagccc gttcggattg tcgctgccgg tgatggccag tgaaatggcg
     7681 ctgatgccat agtggagaaa tttattcatc agctgtccgc tggttaatcc gggataggct
     7741 atggtgaaat aaaaaccgtc agccagttcg cgcgtatcgt cgttataaac aatgtaaaat
     7801 ccgtgcttca gaaaggcttc cttcatcagt cgggccttct gtccgtagag atgcagattt
     7861 tcaacaaaat tcacacgtcc gtcattggca gcgcggagca tggcggcaag ggcatactgt
     7921 gcggtatgtg ctgttccggc tgtggtggca tacaacactc cgtaggggat aaaatgtccg
     7981 agtgtatccg aagtgaaata atttttcagg ttgggatacc ggcgcgtgta ggtttgttca
     8041 ctgaaaatca tcattccaat acgctgaccg gcataactga atatcttgga ggccgaaagc
     8101 aggagaatgt aattgtcggt ataatgtgcc actgtgggtt gatacggact ttcaccgggt
     8161 aaaccgtagt tctgcctgaa atccataccg aaataggcca gatcctcaat cacaatcacg
     8221 tcatatctgg ttgccagctc tccgatgacc tgcaattctt cttcagtaaa gcaaatccac
     8281 gaaggattgt tgggtgacga atagcagatg gagcagatat ctccctgctt caggtattcc
     8341 tccagcttcg ctctcagctt gtcgcctctg aactgaaaaa tatcaaatga cttgaacggt
     8401 acacccatga gatgcagctg cgatttctgc accggaaaac ccggatcgat aaacaatgta
     8461 cagggtttat cgggattgct gcgggcggca gccagcaaag cggcaaaact accctgcata
     8521 cctccgacag tcgggatgca gtgcgcaggc ggtgtatcga ggttcataaa cagttttgca
     8581 aaccgggcga tttcttcctt ggcaaccgga attccctcga tattgggata tgcggctcca
     8641 acaccacgct cgagagcttc cttttcggca tcaatgccta tttgcgaagc tgaaagtccg
     8701 ggaactccca tttccatgtg aataaactct tcgccactct ccttctctat catcgataca
     8761 agtttcccta cttcacgtat ggaaagctgg ttaatatcat ctgtgccaaa ttctgtaaaa
     8821 agtctttcaa tcagttcttt tgaaatcatt gtattcttat tccggtaatt attctattta
     8881 tattttctgc tgataagttt tttcagcgaa tatagttttg ttccttttat ttcggaggta
     8941 atcatttcgg gacccacgta atagcgaaga tatacagctt tcgcgcggct tatcttctca
     9001 ataagttcat cgtcgagtgg atgaatcatg cgtgtaactt ttgtggaact gatactgaaa
     9061 ttctgagctg tatccgtact ctgttccgga aaattgatgc cgtttttcaa atcgttgtca
     9121 tacaaccttt ctttcagcgt gagtggcagc ggaatgggtt ccttatcgat gataatgtac
     9181 atctcattcg aaatgtcaaa acttttcagg ggtatggaaa tgatatcgta cattgtataa
     9241 gtactttgtc cgttttcatc aatttctttc agcagggata tttgctgctg gtaatcctgt
     9301 tcgtgacgtt cgttggtgat aaaactctgc tgcagggtgt aacgggtcct gtccatcagt
     9361 ttgtcgcgct tgtagttaat tccgttggtg ataatactac aacctgaaaa aaccgaaaca
     9421 gaaagcagta cgaaggcaat ttctttcagc cagactttca tagtataatg tttttaagtt
     9481 attacttatt ctttcgttcg tctgtccacc acgcgggtag cttttccttc gctgcgggca
     9541 atggtttttg gttctaccag tgtcaccttc acacttagtc cgatggcgct attgagagcg
     9601 tgttcaattt ttctggaaag gttttgcaga tcgcggatag tgtcggtcag cttgttttca
     9661 tccagttcca cctgcagttc gagtgtatcc aggttgtttt ttcggtccac atacaggatg
     9721 taatgagcgc ttgtttcctg taaatcgagc agtacatgtt ctatttgcga cgggaacaga
     9781 ttgacaccgc ggatgataag catgtcgtca ctgcggcctg tgattttttc catccgtacc
     9841 gttgtccgac cacattcaca tttatcgtag tacagggacg agaggtcgcg ggtattgtac
     9901 ctgatcagtg gtattccttc cttggtgagt gtggtgaaaa cgagttcgcc ttcctgtccg
     9961 taaggtaccg gttctttggt tttgggatca acaatttcgg ggaagaaatg atcttcgtgg
    10021 atatgcagtc cgttatgata aatacaatcg ttggctacac ccggacccat gatttcgctc
    10081 agtccgtaga tgtcgtgagc ggtaatgttc catttgcgtt ccagttcctt gcgcatattt
    10141 actgtccagg gttcagctcc gaacacgccc actctcagct tcatatcact gagcgaatat
    10201 ccgaattcag acatggcatc ggccaggtga agggcatagg aaggtgtaca agccaacacg
    10261 gtagatccga aatcggccat cagctgtaac tgacggcgcg tattgccgcc ggagatggga
    10321 ataaccgagc aacctatttc ctcggcgccg tagtgagctc ccaaaccgcc cgtaaatagt
    10381 ccgtaaccat aagaaacctg cactatatcg tgactgtcga tacccgacat ggtaaaacag
    10441 cgggctacca cttccttcca ggtttggata tcattctttg tgtagccaac cgtagtcggc
    10501 tttccggtcg ttccactcga gccgtgaaca cgcacgatgt ccttcatcgg cacagcaaaa
    10561 aggccaaagg gataattgtc acgtaaatcc tgcttgtagg tgaaaggcag tttagcgata
    10621 tcatcaattc cgtgaatatc agaaggtttt acacctgctt cctccatttt acggtgatag
    10681 aacggcacat tgttataaac ccgggcaacc agttcgcgca agcgttcatc ctgcagtttc
    10741 cgcatggcat cgcgtgacat acattcaatt tctttattcc aaatcataag tccctgttat
    10801 cttcccgcct gcgagaagac gtttatttta taattgttgt aaactgttta tttttatttc
    10861 tgccgcaatc cggcaggaag ctgttcgggt atggttactt cagatcgtct tccgttatgg
    10921 atttcaactt attatcccgg ataacctcca cagctttttc gcggttgtcg gtgcgcaaaa
    10981 ccacgatgga tttagaaccg taactgaaag catacacata ctcaatggag atattggctg
    11041 ttgcaaataa attgaggatg ttattcagcg aacccggtgc tgcgcccatt tccagagaaa
    11101 gtacatcgtg aatgcgaacg gcaaaattct cttcgtgtaa tttatcaaga gccagctgcg
    11161 gatcggaaac gagaatacgc gcaattccgt aatcgcttgt atcggcaatg gttaacgcgt
    11221 taatattaat attgttacgg gctagcacgg caagtacttc attcagatat cccggtttgt
    11281 tttccatgaa aacggataat tgtcggatgg tcatgtttgt ttacttttat gttagaagtg
    11341 ccaattaatt ccagcggacg ggatttcacg aacactttat tagaatttat cagtatttga
    11401 ttgcaaagtt acatatttaa taagaaaaat gaaacagata ttatacaaat atcttattt
//
LOCUS       DAOPYB010000096        11437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6470_length_11437_cov_8.323230, whole genome shotgun sequence.
ACCESSION   DAOPYB010000096 DAOPYB010000000
VERSION     DAOPYB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6470_length_11437_cov_8.323230"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKH58_08235"
     CDS             <1..578
                     /locus_tag="PKH58_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89054.1"
                     /translation="YSSALVYFKKLQTVAQTTENSNIARLGILRCSYFLNNQTETINI
                     ATEIINDPKSSEAVRTEAKLNRAKARVQQNKLTDALKDLKEIVIDTRTSFGAEAKFTL
                     ADVLFKLNRLNEAETEVLDFAKKGTPYQYWLASSFIVLADVYIQKGDDFQAKQYLLSL
                     QKNYTTNDDIQPKIIQRLNEIENRSKVKVVK"
     gene            640..2376
                     /locus_tag="PKH58_08240"
     CDS             640..2376
                     /locus_tag="PKH58_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89055.1"
                     /translation="MKYKILALLIILSSGIVSAQDSIVNRNVTVEREYQPLINDAGKI
                     ITNPKVLEPIVEKTKAVFSDITAPLKIGTNIQTLDPEELKHLPSVVKNGFARVGLGYP
                     LNTLAEFMYPLVKNENNRLDLSLRHLGALTDKIHSKTTTGIQYDHLFSGFNFYTGITG
                     RHDFYNYYGRIFGADKVYQMADITPAYGNAVYTTPEANTLTLRNLSGFPLDETNWRVN
                     FTAGARSLPGAESLNYDISMNYNLFTAISGKLTENELMLKANFDVPFNYNRMGMNVEI
                     HNLNYGSSAIKPNNFPLSYNLIKINPFYKLKGENWLLKLGVKTGLSLGHGQVFTPSPD
                     VTAQWNAVPEYLSLYGGVTGDLTVNTLSRTYDENRYISPGNRLEDLYTPLDAFLGIKL
                     SPASGLMFDVWGDYQIIHNQYLYVNRSYTTSDTTVMEDIKTVYHNRFDVIYAPAGKLS
                     AGMRLNYDYKNRFGAYVKGAYHSWKVENQDFAWQLPVWDSDLGMYAKIKDNISLNTQF
                     IFQDGRYARLGNTARRMNAVYDWNLGASYAYLDWLSVFIKLNNILNSKYDYYYGYQVQ
                     GINAMIGASFSF"
     gene            complement(2471..3946)
                     /locus_tag="PKH58_08245"
     CDS             complement(2471..3946)
                     /locus_tag="PKH58_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89056.1"
                     /translation="MKRFFICMSVLAINAVLFAVTPPPVPQKMSYQAVIRNNSNVLVT
                     NQSVGMKISILQYSATGTAVYAETQTPTSNANGLVTIEIGTGTVVTGTFSTIDWSSGP
                     YFIKTETDPTGGTAYTITATSELLSVPYALFAYSAISLKLPLSISGNFSSTPFTIYNT
                     GTDLYTIKGISTNGFGLYGETKATNGSGVYGRGTGANLSSGVVGVTGEGTTDILPGNS
                     GVLGEANAYIGVAGTALSGTGGYFSSRTGLALNTKGNIKLTGIGEAEGNILTSDASGT
                     ASWKEPLWHQSAAKIYYSGQVGINTTIPNASLHVVGGSEIARFESSANSKWIALYQDN
                     TRKGLLWSVNDDIKLLSDVGGVAFRTAGIDRVTITQEGKTGIGDATPEATLDVEGTMV
                     IGSGGKIFSEIREIIGTTSSTANAVTIQYPTGYTKENTRVLSCEINYNGNAWMGMAGT
                     NTPLNSTSNVLYYLGSSIMIYYPMNEYFQNRAFRMMVMKVQ"
     gene            complement(3951..4448)
                     /locus_tag="PKH58_08250"
     CDS             complement(3951..4448)
                     /locus_tag="PKH58_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89057.1"
                     /translation="MKKLIILFLSIILTGPAITCVYGQQVHASLNASGGNTESAGGSV
                     SYSVGQVFFSTTFSTNGSISEGVQQPFEISVLSGLEDVSGIDLFYTVYPNPTSGKLTL
                     KIEKIDLNQFAYQLFDVNGKMLRNDKLKESETDIEMSDLISSTYFLKVTKDNKEVKLF
                     KIIKN"
     gene            complement(4519..6759)
                     /locus_tag="PKH58_08255"
     CDS             complement(4519..6759)
                     /locus_tag="PKH58_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNX89058.1"
                     /translation="MKNLFITSVLFLFFDVLLFSQSIHAQAPEKLSYQAVIRNSSNTL
                     VVNQPVGMKVSILQGSATGTLIYAETQTPTTNANGLINIAIGTGNAIYCTFSCIDWSQ
                     GPYFIKTEIDPSGGTAYTITSVSELLSVPYALFAGKSGLLTLPYTGLAENSNPGISVF
                     NLGGTAISGTSDKPDSGIGISGSATSQTGSAVGVEGRVSSSMGYGVVGYATSTSGSAK
                     GVYGISNALDGIGVLGESKYIGVRGVNLSTTGYAYGVEGQVNSSDGIAVLGSSISQTG
                     NTVGVYGSSLSSTGKGVYGENKSIGGFNYGVYGFSASSSGTGVYGESYRCGVRGNNNV
                     NTGTAYGVQGNVHSSSGIAVEGTAVSMTGYTVGVYGTSASPNGTGLSGFCSSQTGETK
                     GVVGYINSPDGFSGYFAGGKFHVRGKVGISTETPEYDLDINKQTGFAGLRLKSNDDGV
                     LMTLDQVNDDNNGHYSFIMFRKAGVNLFTVGAAYNEFRIFNANDKGLLVKSTGDLNLT
                     DNLILNSGKRLGIGINTPGAGLHLKGTGYPDAFIFLEAAAGNDAGFRIYEGLTAKWHI
                     FNDFNAGGLRIYNTNAATAIFCKQSNSYVGINTTSPTYNLEVNGTAGKTGGGSWSTSS
                     DIRLKDITGNYTKGLKEIIALQPVSFIYKAGNARNLPTNVLQIGFVAQDVQKIFPEAV
                     TKGEDGYLDFNIHAINVALVNAVKELQTENERLKTENDQLKMIIEKNDVRLTEVENLV
                     RDLIKK"
     gene            complement(7061..9853)
                     /locus_tag="PKH58_08260"
     CDS             complement(7061..9853)
                     /locus_tag="PKH58_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX89059.1"
                     /translation="MKKYILFILYFVLLSCSSNRIITREKKDLSGIWQFATDSADAGV
                     KQRWYLTNLKETVNLPGTTDLNKKGILNTDSSKMHLSRVYKYEGVAWYRRKVTIPESY
                     RGNHIELVLERTKLTEVWIDSVRVGGMKLLQSPHRFDVSRYLTPGEHSITIRVDNTLK
                     YTPYGNVHIYSDDTQTNWNGIIGELLLEVSPTSYIGDLQVKPDVDNKKIAINLSVENS
                     GSIPHGEIEFYLTKMENGKSRKVKTVKQTCQIKQNMTFELNLGKDCSLWDEYKQPLYT
                     LTVVVSANGQKDAKSVVFGMRKFSVTGTQFAVNGRTVFLRGKHDAAVFPLTGHTPMHV
                     EEWERVFRVAKSYGINHYRFHSYCPPDAAFTAADRTGIYIQAELPFWGGLDADSTATM
                     LLNEGIAMIKAYGNHPSFVMFSPGNEIWSGHDRVEKNILAFKNFNNRLLYTMGSNNNI
                     GYAAPRDCSDYFIGARTPYNGDTTLTHTRLTHAFADTREGGILNTQTPSTLTSYSYSV
                     SHLKIPLISHEIGQYQIYPDYNEINKYTGVLRARNLEIFRNRLIKSGMMTQNKDFQKA
                     SGAWAARCYNAEMETAIRTRGMGGFQLLDLQDFPGQGTALVGILDAFMDSKNVISAEE
                     WRQSCNDVVLLAELKKYCWTNREKFTANISVANYSPITIKNTLQWELTDQNGVIIKKG
                     ILNSLKPATGDVTTTGSIEVDLSPIRNAKRLNLHLSLKGNKNYSNSYPIWVYETPDKI
                     TVPENIVVADKLNVDIIRQLENGANVLLFPATDYVLHNSLPGLFPPDFWNYGMFKSIS
                     ESNGKPLSPGTLGILTDPKHPVFHSFPTDYHTNWQWFSIIKASRPMILDQLAKDYKPI
                     VQVIDNLERNHKLGLIFEFKSGKGKLLVCMSQLNKIADKPEAAQLYRSIISYMNSTSF
                     QPVYNISGSELYDLFK"
     gene            complement(10091..10852)
                     /locus_tag="PKH58_08265"
     CDS             complement(10091..10852)
                     /locus_tag="PKH58_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNX89060.1"
                     /translation="MKKTLFTLLIAISLTSCAELLQLANTAGTATSALPVTEAENASG
                     LKESLNVGIVNAVNLLGVENGFYNDALLKILLPEEAQPIIKNIRLIPGGKELVDKAVL
                     SLNRTAEDAVKEATPIFKKAIFSMTIKDAAGILFGGENAATNYLKEKTYSDLQTAFAP
                     KVKNSLAKPLVGDFSTTQSWNSLTSTYNKVAATPVGVVANLKPVNVNLEEYVTGKALD
                     ALFVKIAAEEKAIRENPVARISAILKRVFGQLDKK"
     gene            11181..>11437
                     /locus_tag="PKH58_08270"
     CDS             11181..>11437
                     /locus_tag="PKH58_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HNX89061.1"
                     /translation="MLSTDEKYMARCLQLAQLGGGYVSPNPMVGAVIVCNDRIIGEGY
                     HRHIGEAHAEPNAIYSVNNKELLSQSTLYVNLEPCSHYGKTP"
BASE COUNT         3341 a         2411 c         2186 g         3499 t
ORIGIN      
        1 attattcctc ggcgctggtt tatttcaaaa agctgcaaac agtagctcag actaccgaga
       61 acagcaatat tgcccgactt ggaatactgc gttgctccta tttcctgaat aatcagaccg
      121 aaacgataaa cattgccacc gagataatca acgatccgaa atcgtctgag gcagtacgaa
      181 ctgaagcaaa actcaatcgt gcaaaagccc gcgtacagca gaataagctt actgacgctc
      241 tcaaagacct gaaagaaatc gttatagata cacgcacatc attcggtgca gaagcaaaat
      301 tcaccctggc agatgtgtta ttcaaactca accggttgaa tgaagcagaa accgaagttc
      361 ttgattttgc caaaaaggga acaccctatc agtactggct ggcaagcagc tttattgtac
      421 tggctgatgt ttatattcaa aaaggagatg attttcaggc taaacaatac ctgctcagtc
      481 tgcagaaaaa ttataccact aatgatgata ttcaaccaaa aatcattcag cgtctgaatg
      541 agattgaaaa tcgctccaaa gtaaaagtag ttaaataaaa gggatggaag aatggaaaac
      601 agacataaat catttattta taaaatcaac aatattcaaa tgaaatacaa aatattagca
      661 ttactgataa ttctttcatc cggaatagtt tcggcacagg acagcatcgt caacaggaat
      721 gttaccgtag agcgtgaata tcagccccta ataaacgatg ccggtaaaat cattacaaat
      781 ccgaaagtcc ttgaaccaat cgtggaaaag acaaaagctg tattttcgga tatcactgca
      841 ccgctgaaaa tcggcaccaa tatccagacc ctcgatcccg aagagctgaa gcacctacca
      901 agtgtggtga aaaacggttt tgcccgtgtt ggcctgggtt atccgctgaa tacacttgcc
      961 gaatttatgt atccgcttgt taagaatgaa aataaccggc ttgacctgtc tctgcgtcat
     1021 ctgggagcac tcaccgataa aattcacagc aaaacaacta ccggcatcca gtacgaccat
     1081 cttttttctg gatttaattt ctatacaggt atcactggaa ggcatgattt ttataattat
     1141 tacgggagga ttttcggagc cgataaagtt tatcagatgg ctgatatcac gcctgcttat
     1201 ggaaatgccg tttataccac ccccgaagcc aatacactta cactccgcaa tctttcgggc
     1261 tttccactcg atgaaaccaa ctggcgcgta aatttcacag ccggagcacg ttcactgccc
     1321 ggtgcggaat cgctcaacta tgatatcagc atgaattaca atctgttcac tgccatatcc
     1381 ggtaaactca ccgaaaatga attgatgctg aaagccaatt tcgatgttcc tttcaactat
     1441 aaccggatgg gaatgaacgt ggaaatccat aatctcaatt acggaagttc ggcaatcaaa
     1501 ccaaataact ttccattgag ttataacctg ataaaaatta atccgtttta caagctaaag
     1561 ggcgaaaact ggcttttgaa acttggtgta aaaaccgggc tttcgctcgg ccacggacag
     1621 gtatttactc catcacccga tgttactgca caatggaatg ccgttccgga atacctttcc
     1681 ttatacggag gagtaacggg tgatctgacg gttaacacgc tgagcagaac ttacgacgaa
     1741 aaccgctaca tcagtccggg caacagactg gaagatctct acacaccact tgatgccttt
     1801 ctgggcataa agctcagtcc tgcatcgggt ctgatgtttg atgtgtgggg cgattatcag
     1861 attatccata atcaatatct gtacgtcaac cgatcttata ctacttccga cacaacggta
     1921 atggaagata taaaaacagt gtatcacaac cgttttgatg taatttacgc acctgcagga
     1981 aaactttcag ccggaatgcg gttaaactat gattataaaa accgtttcgg tgcttatgtt
     2041 aagggagctt atcacagttg gaaagtcgaa aatcaggatt tcgcctggca actgccggta
     2101 tgggattctg atctgggaat gtatgcgaaa atcaaggaca acatcagcct gaacacacag
     2161 tttatttttc aggatggccg ttatgcacgt ttaggcaata cagcccgaag aatgaacgcc
     2221 gtatatgact ggaatttggg agcatcgtat gcctacctcg actggctttc agttttcatt
     2281 aaactgaata acattttaaa ttccaaatac gattactatt acggatatca ggtgcagggc
     2341 atcaatgcaa tgatcggagc atcattttcg ttctaagagt aactgtattt aaaagaaaag
     2401 gctgtctcaa aactaaatgg agacagcctt tatttttatg gttgataaga aaattgttac
     2461 agaaaatgat ttattgcact ttcattacca tcatccggaa agcacgattc tgaaaatact
     2521 cattcatagg ataataaatc attatggaag atcccagata ataaagtaca tttgaggtgc
     2581 tgtttaatgg agtatttgtt cctgccatgc ccatccatgc atttccatta taattgattt
     2641 cacaggaaag aacccgcgta ttttctttgg tatatcccgt tggatactga atagtaactg
     2701 cattagctgt cgatgaggtt gttcctatta tttcccttat ttcggagaag atcttaccac
     2761 ctgaaccaat aaccatcgta ccttccacat caagcgttgc ttcgggtgtg gcatctccta
     2821 ttccggtttt tccttcctga gtaattgtaa ctcgatcaat tccggctgtc ctgaatgcca
     2881 ctcctccgac atcgcttagg agtttaatgt catcgttgac gctccatagt aatccttttc
     2941 tggtgttatc ttgataaagt gctatccatt tactgttggc tgatgactcg aatcgggcta
     3001 tttcagatcc acctacaacg tgtaatgaag cattaggaat agttgtatta atgcctactt
     3061 gtccggaata ataaattttt gctgctgatt gatgccagag tggttctttc cagcttgcag
     3121 ttccggatgc atcagaagtc agtatattcc cttccgcttc accaataccg gtcagtttta
     3181 tatttccttt tgtattcaat gccagtcctg ttcggctcga gaaatagcca ccagtaccgg
     3241 ataaagctgt accggccact ccgatatatg cattagcctc accaagtact ccggagtttc
     3301 ccggaagaat gtctgtagtg ccctcaccgg taactcctac gacaccggac gataaattgg
     3361 ctccggttcc ccgcccgtaa actcctgatc catttgtagc ttttgtttcg ccataaagac
     3421 caaatccgtt tgtactaatc cctttaatgg tataaagatc agttccggta ttatatattg
     3481 tgaatggtgt gctcgaaaaa ttccccgaaa tactcaaagg tagttttaac gatatggctg
     3541 aatatgcaaa caaagcataa ggcacactga gcagttcact ggtagcggta atggtgtatg
     3601 ctgttccgcc tgtcgggtca gtttcagttt taataaagta aggaccggac gaccagtcaa
     3661 tcgtagaaaa tgttccggtt accacagttc cggttccaat ttcgatagtt accaatccgt
     3721 tagcattcga agtcggtgtt tgggtttcgg catacactgc ggttccagtc gcggaatatt
     3781 gaagaatact aatcttcatt ccaaccgatt gattggttac cagcacattg ctgttgttac
     3841 gtattacagc ttgataactc attttttgag gaacaggagg tggggtaaca gcaaaaagca
     3901 cagcgttaat cgccaataca gacatacaga taaaaaatct tttcattgct ttagtttttt
     3961 atgattttaa ataatttcac ttccttatta tctttagtta cttttaaaaa ataagttgac
     4021 gaaataagat cggacatttc aatgtcagtt tcactttctt tcaatttatc gtttctgagc
     4081 atcttgccat tgacatcgaa caactgatat gcgaattggt tgaggtcaat tttctcaatt
     4141 ttcaaagtga gttttccgct cgttggatta ggataaacag tatagaacaa gtcaatgcct
     4201 gacacatcct ccaatccgga caatacagag atttcgaacg gttgttgtac cccttcggat
     4261 atagaaccat tggtactgaa tgtagtcgaa aaaaacacct ggcctaccga ataactcacc
     4321 gatccaccgg cactttctgt atttcctccg gaagcattca atgaagcatg tacctgctga
     4381 ccatatacgc aggtaatggc gggccctgtc aagataatgg acagaaaaag aatgattaat
     4441 tttttcatga tatatgtttt aggaaattat tattattgtt ttaccaaata taatctgaag
     4501 aatgacatag aaatcaatct attttttaat caggtcgcgt accaagttct caacctcagt
     4561 aaggcggaca tcgttttttt caattatcat ctttaactga tcattctctg tttttagcct
     4621 ttcattttca gtttgcagtt ctttgactgc attcactaat gctacattaa tggcatgaat
     4681 attaaaatcc aggtaaccat cttcaccttt tgtaacagct tccggaaata ttttctgaac
     4741 atcctgtgct acgaatccaa tttgaagaac gttggtgggg agattacggg cgttgcctgc
     4801 tttgtatata aatgatactg gttgtagtgc aataatttct tttaatcctt ttgtatagtt
     4861 tccggtaata tctttcagtc ggatatctga cgaagtgctc caactgccgc ctccggtctt
     4921 cccggcagtg ccgtttactt caagattata tgtaggactg gtggtgttga tgcctacata
     4981 cgaattggat tgtttacaaa agatagcagt tgctgcattg gtattatata tcctcaatcc
     5041 tccggcattg aaatcattga atatgtgcca tttggcagtc aatccttcat aaattctgaa
     5101 acctgcatcg tttccggccg ctgcttccag aaatataaat gcatcaggat atccggttcc
     5161 tttaagatgt aaacctgcac ccggagtatt aatgcctata cccaatcttt tcccagaatt
     5221 caggatcaga ttgtctgtca ggttcaaatc acccgttgat ttgacaagca aacctttatc
     5281 attagcattg aaaatgcgaa attcattgta agcagcacca accgtaaaca gattgacacc
     5341 cgcttttctg aacataataa atgaataatg accgttattg tcatcattta cctgatccag
     5401 agtcatcaat acaccatcat cattcgattt aagccttaat ccggcaaatc ccgtttgttt
     5461 atttatatca agatcatatt caggtgtttc tgttgagata cccactttgc ccctaacatg
     5521 aaatttacca cctgcaaaat aacctgagaa accatcaggt gaattaatat aaccaacaac
     5581 tccctttgtt tcacctgtct gtgaactaca aaatccggat aaaccagtgc catttggtga
     5641 tgctgaagtt ccgtaaactc ctactgtata tcctgtcatg ctcaccgccg taccttccac
     5701 agctatccct gacgaagaat gtacatttcc ctgaactcca tatgctgttc cggtattaac
     5761 attattatta cctcttaccc cacaacggta tgattcccca tacactcctg ttcctgaaga
     5821 agatgcagaa aaaccgtaaa caccataatt aaatccgccg atagatttgt tttccccata
     5881 aacaccttta cccgttgacg ataatgacga accatatact cctacggtat tacctgtctg
     5941 acttatggaa gatcccagga ctgcaatacc atccgaactg tttacctgac cctcaactcc
     6001 ataagcatag cctgttgttg ataaattgac acctctgaca cctatgtatt tactctcccc
     6061 caagacccca ataccatcca gagcattaga aattccatat acacctttag ccgaaccgga
     6121 agttgatgta gcataaccaa ccacaccgta tcccattgag gaagataccc tgccttctac
     6181 accgacagca ctaccagttt gcgaagttgc gcttccactg ataccaatac cactatccgg
     6241 tttgtctgaa gtgcctgata tggcagtacc tcccagatta aaaactgata ttccgggatt
     6301 tgaattttct gccaaacctg tgtaaggtaa agtcaacaaa cctgacttcc ctgcaaaaag
     6361 tgcataagga acacttagca gttcactaac agatgtgatg gtataagccg ttcctcctga
     6421 aggatcaatt tctgttttga taaagtatgg accctgtgac cagtctatgc atgaaaatgt
     6481 acaatagata gcattcccgg ttccaatggc aatatttatc aatccgtttg catttgttgt
     6541 tggagtttgt gtttcagcgt agataagagt tcctgtggct gaaccctgta gaatgcttac
     6601 tttcattcct accggttgat taactaccag tgtattactg ctgttacgga taacggcctg
     6661 ataacttaac ttttcgggtg cctgagcatg gatggattgt gaaaataaaa gaacatcaaa
     6721 aaacaaaaaa agaactgacg taataaatag gtttttcata cgacataggt atttgtgtaa
     6781 gtatatagaa atcagataaa aatacaagta tcaaaatata tgatgagaag caataagtat
     6841 ataattcttg tatataaagt ttgatagaat gacttatatg tatattctat tctcacttat
     6901 catgattctt cccgtatcgc caaggagaat tacaacaaat tcatattcag tatttaaaca
     6961 caattccagg gtaattgttt gaagtaaatc taatgtttct taaaatttat tttatttgaa
     7021 ggaaaatctt tgtttatttc attcaagcat atcatttaat tcatttaaac agatcatata
     7081 gttcactgcc gctgatatta taaacaggct ggaaactcgt tgaattcata taactgatta
     7141 ttgacctgta aagctgagca gcttcgggct tatcggctat tttattaagt tgcgacatgc
     7201 ataccagcaa tttacctttt cccgatttga actcaaaaat cagccccagt ttatggttcc
     7261 tttccagatt gtcaattacc tgcacgatgg gtttgtagtc ttttgctaat tggtccagaa
     7321 tcatcggtcg ggatgcttta atgatggaaa accattgcca gttggtatga taatcggtag
     7381 gaaatgaatg aaaaaccgga tgtttcggat ctgtcagaat accaagagtg ccgggcgata
     7441 acggtttacc attcgattcg ctgatacttt taaacatgcc atagttccag aaatcaggcg
     7501 ggaataatcc cggaaggctg ttatgtagaa cgtaatcagt cgcaggaaac aacaaaacat
     7561 ttgctccatt ttccaattga cgtataatat ctacatttaa tttatcagct acaactatat
     7621 tttcaggaac agtaatctta tcaggagttt cgtataccca tatcggatag ctgtttgaat
     7681 aatttttatt tcctttcagg ctgagatgca aatttaaacg tttggcattc cggataggtg
     7741 ataagtccac ctcaatgctg cctgttgttg taacatcacc tgtcgccggt tttaatgaat
     7801 tcagaattcc ttttttaatt atgacgccat tctggtctgt taactcccat tgaagtgtat
     7861 tctttattgt tataggagaa tagtttgcca ccgaaatatt tgctgtaaat ttttcacgat
     7921 tggtccagca gtattttttt aattcagcca acagaaccac atcgttgcag gactgacgcc
     7981 attcctcagc cgaaatcaca tttttgctgt ccataaaagc gtcgagtatg cctaccaaag
     8041 ctgttccctg tcccggaaaa tcctgaagat cgagcagctg aaatccgccc atgccgcgcg
     8101 tacgaattgc tgtttccatt tcagcattgt aacaacgggc tgcccatgca ccggacgctt
     8161 tttgaaagtc tttgttctgc gtcatcatac cggatttaat aagacgatta cgaaaaattt
     8221 ccagatttcg cgcacgaagc acacctgtgt atttatttat ttcattataa tcgggataaa
     8281 tctgatactg acctatttcg tgactaatca ggggaatttt gagatgtgaa acggaataac
     8341 tgtaactggt cagtgttgaa ggtgtctgtg tattaagaat tccaccttca cgagtatcgg
     8401 cgaatgcatg tgttagcctt gtatgggtta gcgttgtatc accgttataa ggtgttcttg
     8461 cacctataaa gtaatcggaa caatcgcgtg gtgcagcgta accaatgtta ttgtttgaac
     8521 ccatggtata aagcagccgg ttattgaaat ttttgaaagc cagaatattc ttttcgactc
     8581 tgtcgtgtcc gctccatatt tcattcccgg gtgaaaacat aacaaacgat ggatgattgc
     8641 cgtaagcctt aatcatggca attccctcat ttaatagcat tgttgcagta ctatcggcat
     8701 ccagtcctcc ccaaaaaggc aattcagcct gaatataaat tccggttcgg tcggcagctg
     8761 taaaagcagc atctggtgga caatatgaat gaaaccggta atgattgatg ccgtatgatt
     8821 tggcaacgcg aaatacacgc tcccactctt ccacatgcat cggcgtatgt cccgtgagcg
     8881 gaaagacagc ggcatcgtgc tttccccgga ggaaaacagt ccgcccgttg acagcaaact
     8941 gagtaccagt caccgaaaac tttcgcatac caaaaacaac tgattttgca tctttttgtc
     9001 cgtttgctga tacgaccaca gtaagagtgt aaaggggctg cttgtattcg tcccagagac
     9061 tgcaatcttt tcccaggtta agttcgaatg tcatattctg ctttatctga caagtttgtt
     9121 tcactgtttt gaccttgcgg gatttcccat tttccatctt tgtcagataa aattctattt
     9181 cgccatgagg aattgatcct gaattttcaa cagataaatt gatagcaatt ttcttattgt
     9241 caacatccgg ttttacctgc aaatcgccaa tataacttgt tggggaaacc tcaagaagga
     9301 gttcgccgat tatgccgttc cagttggttt gtgtatcgtc agaataaata tgaacgttgc
     9361 cgtagggagt atatttcagc gtattgtcaa cgcgaatggt aatagaatgt tcgccgggag
     9421 tgagatatcg cgacacatcg aaacggtgag gtgattgcaa aagtttcatg ccccctacac
     9481 gtacactgtc aatccatact tctgtgagtt tggtacgttc gagcaccagt tcaatatgat
     9541 taccacggta actttctgga attgtaacct tcctgcgata ccacgctaca ccttcatatt
     9601 tgtacacccg gctgagatgc atttttgaac tatcggtatt gagtattcct tttttattca
     9661 aatcggttgt acccggcagg tttacagttt ctttcagatt agtgagatac cagcgctgtt
     9721 tcactccggc atccgctgag tcggttgcaa actgccatat tccggacaaa tccttttttt
     9781 cgcgggtaat tatccggttg cttgagcagg ataacagaac aaagtacaag ataaagagaa
     9841 tatatttttt cacgaaattg ttcattaaac tatacaaatg attaataata tttttcaaaa
     9901 atataaaaaa ttttatataa aaatgctatt caataaaaaa aacggacaaa ctctgtgttt
     9961 gatccgtttc taattatact tttaaaagaa gctggttttt aaatctgtat gatacaattc
    10021 tttgcttttc aatcgacatc aataattatt gaaattttga aaaaaaggaa ttcttatttt
    10081 gtttgataaa ttacttttta tcaagttgtc cgaatacgcg tttgaggatg gccgaaatac
    10141 gtgctaccgg attttcgcgt atagcttttt cttcagcagc aatttttaca aaaagtgcgt
    10201 caagcgcttt accggtcaca tattcttcca ggttcacatt tacaggttta agattggcaa
    10261 ctacacctac cggcgttgca gccactttgt tgtaagtcga tgtcagcgaa ttccacgact
    10321 gtgttgtcga aaaatcgccc acaagcggtt ttgccaatga atttttcact ttaggtgcaa
    10381 aagctgtctg caagtcggaa taggtttttt ctttcaggta attggtagcc gcattttcgc
    10441 ctccaaataa aataccggca gcatctttta tcgtcatgct gaaaatggct tttttgaaaa
    10501 ttggagttgc ttccttcaca gcgtcctcag cggtgcggtt gagcgaaagg accgctttgt
    10561 ccaccagttc tttgcctccc ggaataagcc tgatattttt tataattggc tgtgcttctt
    10621 cggggagaag aattttgagc agggcatcgt tgtaaaaacc gttttccaca ccaagcagat
    10681 taactgcgtt tacaattccc acattcagtg attctttaag tccgctggcg ttttcggctt
    10741 cggttaccgg cagcgccgat gtggcagttc ctgctgtatt agccagctga agcagttcag
    10801 cgcacgatgt caggctgata gcaattaaca aggtaaataa tgttttcttc atacttttat
    10861 gtttgatttt taaaaatttt ttcacgaatc tcagttcaga ggtaaattgt tttttttagg
    10921 actgacagtt atatttttca aagttaaaat tttattgaga attactcaaa aattattcca
    10981 caaaattgtt tgttagcgat tagtttatct tctgtgggag tttccaagtg tttcacagcc
    11041 aaaaaatgga agctattctg tgcttttata aaaacacaac tgcttcttca cgtaacaggg
    11101 tataaaaaat ccgtttattt aatcgggaaa ttgtatgaaa tatcgtattt ttgttgtcag
    11161 ttgaattttt aactattcga atgctgagta ctgacgaaaa atatatggcg cgatgcctgc
    11221 aactggcaca gttggggggt ggttacgttt caccaaatcc gatggttggc gcagtaattg
    11281 tatgcaacga ccgcattatc ggtgaaggtt atcaccggca tatcggcgaa gctcatgccg
    11341 aacccaatgc catttattcg gtaaataata aggaattatt atcccaatct accctttacg
    11401 tcaatctgga accttgctct cattacggga aaacgcc
//
LOCUS       DAOPYB010000097        11266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6590_length_11266_cov_7.716172, whole genome shotgun sequence.
ACCESSION   DAOPYB010000097 DAOPYB010000000
VERSION     DAOPYB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11266
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6590_length_11266_cov_7.716172"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..365
                     /locus_tag="PKH58_08275"
     CDS             72..365
                     /locus_tag="PKH58_08275"
                     /inference="COORDINATES: protein motif:HMM:NF018045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89062.1"
                     /translation="MKKGLLKLSVIFALAIMMTSCYTITATVGNGPQTGTKIVAHNHY
                     LINGLAPISTANTKELVGDSKDYSITISHSFIDGVLSLITGSIYSPTTVTITK"
     gene            839..1333
                     /locus_tag="PKH58_08280"
     CDS             839..1333
                     /locus_tag="PKH58_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNX89063.1"
                     /translation="MKKITSSISLVLALSVLFASCSSTTMIQSTPSKAKLYLDNQYVG
                     ETPYTHMDSKIVGSSMQVKLEKEGYKPFITTITKDEEVNVGAIIGGIFVLVPFLWTMQ
                     YQPVHNYELKPLTSDNETNVVPLASQKSKADKLRELKHLLDEKIITTEEFEKEKAKIL
                     QSND"
     gene            2507..4222
                     /locus_tag="PKH58_08285"
     CDS             2507..4222
                     /locus_tag="PKH58_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89064.1"
                     /translation="MKTTKLLKCALYMAVLAAFGLVGCEKLDLYSIDSPSDLQRRIDS
                     IAASRPDTGDTTYVAIATAIVGAEDNSAGWWTAFTDYFTIPTNKLLHMEFINHSSGAG
                     NWNNWNLAVTNEIADRSAADYKEYFVLRSDAYGWGGKMANVDPAYAYNSKMITQNYPD
                     TDGDGDIWNDFRSTMQGARVTIEIDHSATGNVFVTATAVGKNGVTLVETYQQPVSASA
                     DIVAFLIADGSHFEMKKAYLIPSKVTVVEDVNPVSIAVEGTPAFVELGNQNFWGDAKA
                     TVTFADGSTKQVDSVDISFNVIPDMSKLGKKTVVVAYNKTKQGAYCKAVSTFYTLEVT
                     NPVVSMEVTTMPDITKYYYFNSDPILFNTKGIVVTGTYSDGSKAVLPNETLKFGKIPA
                     AAGTQAVTITYTGATKIVTTTCPLTLVKGIGQVGATDLSTAWWTAFSSDYKVEPGASK
                     KITMYCYSDNLANWHSPCTILRKADKTEYAVVRMDNFGWGNSYAASTKTSDWNWDTFT
                     SNINGSKVIITVTNNGNNTADILYNVTYANGETHFQKYAGLAVDSNDLNCALVTEVSY
                     LVIVE"
     gene            4338..7475
                     /locus_tag="PKH58_08290"
     CDS             4338..7475
                     /locus_tag="PKH58_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX89065.1"
                     /translation="MFQTDNITVKGKIVDDKGEPLPGATVKQKGTSNGTISNLDGNFT
                     LTVPADATLNVSFLGYKTLEISVGGKAEIGTITLESDVKELDQVVVIGYGTQRKVDLT
                     GSVAIVNTEEMKKVSHSNISTMLEGKVAGVQITSDGQPGADPTVRIRGIGSFGSTAPL
                     YIIDGVPMGTTIRDFSPNDIETLQVLKDASATAIYGSRAANGVVIITTKQGKKNQAMK
                     IDYSGYTGFDQVQKGVYNVMDSYQYGQYVNMAYSNSGMDVPAGYNPASPKYVDPSQVN
                     TNWYNEAFKTGIRQNHNINMSGGGANNTYNIGLDYFSQRGTMEGAGPNFDRFTARINN
                     TMDVKFIKFKTNMVYSHSSQDNMALSNANEYVQGLYGAQYPVMASVLLLPPTIKAYDP
                     STWVLDDRISAATNYSYDSYGYGTYYDDVHGDIRVTNVLLENNLIKRNTIVDRVVASG
                     SADVDLLGMFRLKSSNHKLNYNLNLSYSKTMAKDFTFVPAFIQSTTNYLAKSNENLSE
                     GYRSFSDALIENTVTYDGKFGLSHINAVVGQTFERELYHTLTGKGVTMPEPYYLQVNN
                     AEETSSTSFESEHVLASYIGRINYDFDQKYLLSATARRDGSSRLSSADRWDWFPSVSA
                     GWRIDRENFFPVSKSTINLLKLRGSYGELGNENIGEYQYMDVMSRGNYTYSFGNTKVT
                     GSAISNYVNEAIRWEKKKTIDAGLDLGMFNNQLEFTFDWYKSTSEDLLYSVAVPTSAG
                     ATNGTVTMNAATMQNTGLEFLLAYHNHKHAVKYDISANISTIKNKVTKLGVSGEPRTD
                     GYCRTEVGREVGSFYGYIYEGIFQSQAEIDNRVNSEGNHINQPGAQPGDVIYADIAGD
                     FDANGNPIPDGEITNEDQKYLGSGLPKISYGLSGRAEWKGFDLSVSTYGAAGFKAVDF
                     VDVTLHSSYGALNKSVDLLNAWTPDNTNTNVPRIAYKSTGSITNDMFSERFIQDASYL
                     KIANIELGYNFPDKWFGGYVNGLRIYASAQNLATLSKYKGYNVDFAGGTFTPGYNYCS
                     YPTPRTVMFGAHFSF"
     gene            7506..9260
                     /locus_tag="PKH58_08295"
     CDS             7506..9260
                     /locus_tag="PKH58_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX89066.1"
                     /translation="MKSIKIFLTMLAFIGGMTACNDQLDVQNPNNQTTYDFGNTESDL
                     KEAIISCYNRIRLEGTFARVGYTMDAVRGDEVWNSSQQWYLEYDNLNSPGTIGIGDEW
                     IWRDNYHVVNRTNFVLSKVDNVSMSEASYNQIAGQALFLRSLAYYELATYYQNVPLIT
                     DYASYSDINTMYAPNNTQDQVFDQIEADLTKAMQMLPSRDKGGEWAKGRATSGAAAGY
                     LARALMFRHKYSEAYTVLKDIIAGKYGHYELTADYGDNFREGAKYENNSESLFEVQFL
                     DYGTGGTDEEWTPVNISSEATQGHAVESNYASQEMGSWGDLAASPWLYNLFKKENCTD
                     GRLDPRLYWTLVTFESEYNNYTNLKTAAYPNGDPRCNTIYQKDITATPLSNTAQGGVS
                     IAKFTNARNNIYSSITNGLHCGINLRLMRYSDVLLRAAECENELHGPTQTAIDYINTV
                     RRRVALKDLKLSDFASADALFEQIANVERPKEFGCENGRGIDLLRWGFFYDAGRLNQL
                     VEHGYYKLDGKASTEELTAATATSSSYKYYYKGHEYFPIYQSTLNANPNLTGNSANKN
                     EDNGPAFKAKWNIHPVVK"
     gene            9346..11004
                     /locus_tag="PKH58_08300"
     CDS             9346..11004
                     /locus_tag="PKH58_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HNX89067.1"
                     /translation="MRLKYLLILSVSIASLFFAGCKKDEEPKPDPEPVPGFTIPIYAD
                     DYSSISSWANRNKWNLANVHDPSVAWYNGYYYMYGTDASYGNAHEGHGHFQGKRSTDL
                     VNWEWVGGPFYQAPTWVTDSLNAIRLRMGLAAIAKENIRYGYWAPVVRRVNVGGKDVL
                     RMYYSIVIDNYIKTGKANTTANFDGSWTERAFIGMCESTDPAGAVWTDKGFVTSSSSD
                     RGKNYSRASTNDWSAYFYYNAIDPTYIVTPDGKHYLIHGSWHSGFALLQLDATTGKPL
                     NTLGEPYANSVSELTARYGVRIGTRTASSRWQGSEAPEIIYKDGYYYLFMAYDGLDVP
                     YNTRVVRSTKIEGPYLDITGRNFTNGAGDCYPIVTHPYKFNLSYGWVGISHCCIFQKE
                     NTNEWFYMSQGRLPANVGGNAYSNAIMMGHVRRIVWCPASASEPDNLWPIALPERYAA
                     VPDFGTITKDSLTGTWEHINLKYSYGVQNSATSLTLKADGTMSGALTGSWNYDVAKKQ
                     LKLGNVIVCVEREVDWEASPRCVTFVYAGTEKNLNATYWGKKVK"
     gene            11042..>11266
                     /locus_tag="PKH58_08305"
     CDS             11042..>11266
                     /locus_tag="PKH58_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89068.1"
                     /translation="MKINIFRSVINAVVLSSFIPSMCIQAQNQHIVVHDPVAIESEGT
                     YYIFNTGRGIASLKSNDLKNWQFMKPVFDVS"
BASE COUNT         3566 a         2327 c         2335 g         3038 t
ORIGIN      
        1 atctgataga tggcttccct tttttattgc gctatttttt gtcggtcagt agtgtaaaaa
       61 aataattatt tatgaaaaaa ggtttattga aattgtcagt cattttcgca ttagctatta
      121 tgatgacttc ttgttacact attacagcca ctgttggcaa tggtccgcaa accggaacaa
      181 aaattgttgc tcacaaccat tacctgataa atggtttagc tccaatatca acagctaaca
      241 ccaaagaatt ggtaggcgat tctaaagatt attcaattac aatttctcat tcatttattg
      301 atggggttct ttcattaatt accggaagca tttattcacc aacaaccgtt actataacaa
      361 agtaagtgat atagttgaca cggcagatta aagactaatc tgccgtgttc taaaaatcaa
      421 ctaaatgatt cagccattta aaaatatact caagaaaaga ataataaatg gagtattcac
      481 tcaaaacaag ttcaggaaag atctgtgtac tttatgtgaa aaaaaatgaa cacatgaaac
      541 gagttccgat tattcaggga cgagttctcc cgatatattc ccgataaatc atgacacgaa
      601 gagacaaatt atgcgacaaa aaaaataaat aagaacgcat gaaatatatt ttattagtag
      661 tatcagtaat cctcattttt taccctctat atgcaataat aagttgcatc agatctttca
      721 acagtttaac aaattatgga tatggggtgc ttacaggagg tattatactc ttgattgcag
      781 gctgtttatc catgtatttt actgtcaaat tattcaaaca aaaaaggaaa caatagaaat
      841 gaaaaaaata acaagttcaa taagtttagt gctggcatta tctgttttat ttgcaagttg
      901 ttcgagcacc acaatgatac aatcaactcc gagtaaagct aaattatatc tcgacaatca
      961 gtatgttggc gaaacacctt atactcatat ggactccaaa atagttggta gttcaatgca
     1021 ggtaaaatta gagaaagaag gctataaacc atttattaca acgattacaa aggatgaaga
     1081 agtaaatgta ggagccatta tcggaggtat ttttgtttta gtcccatttc tttggacaat
     1141 gcaatatcaa cccgttcata attatgaatt aaaaccttta acatcagata atgagacgaa
     1201 tgtggtgcct ctggcaagtc aaaaatcaaa agctgacaaa cttcgtgaat taaagcactt
     1261 gctcgatgaa aaaataatta ccactgaaga atttgaaaaa gaaaaagcca aaatacttca
     1321 atcaaacgac taatcccaca gaacctgaat aattattatt tcttgggata atgggacaga
     1381 tatgaacctg aatggcagcc gtattctgtt ctgttaaaaa tatggttaat aaacattcat
     1441 acagtaaagc gggtgaaaaa aggtaaacaa taagagtgaa tgactcggta gtattgtagc
     1501 tatttaaaac tatccatttt tcattagaaa acagtttttt aaataaagca atcaagaaac
     1561 tggcaataat taaaaattat tgccagtttc ttgattttaa atatatttat aaacaccaga
     1621 taatcaaatg tataaagaaa ttgcatgttg tttttttaca aaatgataaa aatattgcat
     1681 ggaaatatgt tgcataacac attaaaaaaa aaaccaactc aagaaataat atatattttt
     1741 gtcacgaatt attaagtgtt catcgtacac ttaatatatg tggctctcat ctgagattct
     1801 tcatattata aaaacagtag attttaagtc atttacgaaa tttacatcta agaaaactga
     1861 atattcaatt atataaattc atacttaaca acttagtgtt tttcagaatt ccgataaatt
     1921 attgaaatta attttcttgt ttaataattt gttatcattt gaataattta caggctgagt
     1981 atttcaaatt attttctgtt ttttaacgat attcaaatga acaatttttt attactctct
     2041 tactaatgta tgatggattt agtcaatata ataactattt tatatgaaaa attctacact
     2101 attattatca gattcgtatt tcggcctgac atcgtatgac cggccgagaa tatgtgatct
     2161 gcagataggc gacgattcga acaagggacc acccggaata taagctgctg attttgcttg
     2221 tcggacaggt gaatttctgc tcattggaaa tgatgaaaca gacaacttta tcattccgtc
     2281 acctccggat taaaggggaa aggcaggaat gacaaactgc cggacgcgcg aaaaacacaa
     2341 ctactaactc tttcagaaag ttaaatcagc gtgttggttg caagactaac tacccggata
     2401 tatcaataaa aaaaaagaat gaatccaatt aattataaat ttatgtaacg attcccgttt
     2461 aaccggagta gttacatctg atcattaaaa aacaattaat ttacccatga aaacaactaa
     2521 attattaaag tgcgctttgt acatggcagt attggcagca tttggcttag ttgggtgcga
     2581 gaagttggat ctgtattcaa tcgattcgcc ctcagacctt cagagaagga ttgactccat
     2641 tgctgcttca agaccagaca caggtgacac tacttacgtc gccatcgcta ctgcgatagt
     2701 cggtgctgaa gataacagtg ccggttggtg gactgcgttc actgattatt tcactattcc
     2761 cacaaacaag ctattgcaca tggagtttat taaccactct tcaggcgctg gcaactggaa
     2821 caactggaac cttgctgtaa caaatgaaat tgcggatcga tctgccgccg actacaaaga
     2881 atactttgtt ctccgttcgg atgcttacgg ctggggaggt aaaatggcga atgtagatcc
     2941 tgcttatgcc tacaacagca aaatgattac ccagaattat cccgacacgg atggagacgg
     3001 cgatatctgg aacgatttcc gctctaccat gcagggtgcc cgtgttacaa tcgaaattga
     3061 ccactcagct accggtaatg tgtttgtaac agccacagcc gttgggaaaa acggtgtaac
     3121 actggtcgaa acctaccagc aacctgtttc agcctctgca gatatcgtgg ctttcctgat
     3181 tgccgacggc agtcatttcg aaatgaaaaa ggcatatctg ataccttcta aagttactgt
     3241 tgtagaagat gttaacccgg tttctatcgc tgtggaagga acacctgctt ttgttgagct
     3301 gggcaatcaa aacttctggg gagatgcaaa agccactgta actttcgccg atggctctac
     3361 gaagcaagtt gactctgttg atatatcatt taatgtcatt ccggatatgt caaaactcgg
     3421 caagaagaca gttgtcgtag catataataa aacaaaacaa ggcgcttact gcaaagctgt
     3481 atctaccttc tacacacttg aagtaactaa tcctgttgta agtatggagg taactacgat
     3541 gccggacata accaaatact attacttcaa cagcgacccg attctgttca acacaaaagg
     3601 aattgtcgta acaggaactt attcggacgg ttcaaaagct gtattgccaa atgaaactct
     3661 caaatttggc aagataccgg cagccgcagg tactcaggct gtaaccatta cctataccgg
     3721 tgcaacaaaa atagtaacta caacctgccc gttaactcta gttaaaggta ttggccaggt
     3781 aggcgctacc gatctatcaa ctgcctggtg gacagcattc tcctccgatt ataaagttga
     3841 gcctggtgca agtaagaaaa ttaccatgta ctgctactcc gacaatctgg ccaattggca
     3901 cagtccgtgt acaatacttc gcaaagccga caagacagaa tatgcagttg ttcgtatgga
     3961 caacttcgga tggggcaaca gctatgccgc atcaaccaaa acatcagact ggaactggga
     4021 tacattcact tcgaacatta atggttctaa ggtaatcatc actgttacta acaatggtaa
     4081 taacactgct gatatacttt acaacgttac ttacgcgaat ggcgaaacgc atttccagaa
     4141 atatgcaggc ctggcggttg acagtaatga tttgaactgc gcacttgtaa ctgaggtgtc
     4201 gtatctcgta attgttgaat aatcactaag ataaaaatat ttaaaccatg aaatattcat
     4261 atttaatctt gctgggattg ctggcgttaa catcggcaac ttatgcaact accaacggca
     4321 ataccaatca gttgtcaatg tttcagacag acaacataac agttaaaggt aaaatcgttg
     4381 atgataaagg agaacccctt ccgggcgcga ctgtaaaaca aaaagggaca agcaatggta
     4441 caataagcaa ccttgatggt aactttactc ttactgttcc tgcggatgct acgttgaatg
     4501 tatctttcct tggatacaaa accttagaaa tttccgttgg aggaaaagcc gaaataggaa
     4561 ccatcacgct ggaatcggat gtaaaagaac tcgaccaggt ggttgtaatc ggttacggaa
     4621 cacaacgcaa agttgacctg actggttcgg ttgccattgt caacaccgaa gaaatgaaaa
     4681 aagtatcgca ctcaaatatc tcaacgatgc tcgaaggtaa agtcgccggt gttcagatta
     4741 catccgacgg acaacccgga gccgatccta cagtacgtat ccgtggtatc ggttcattcg
     4801 gaagcaccgc accgctctac attattgatg gagtgccaat gggaacgaca atacgcgact
     4861 tttcgccaaa cgatatcgaa accctacagg tactgaaaga tgcttctgct acagctattt
     4921 atggttcgcg tgctgccaat ggtgtggtta tcatcaccac taaacagggt aaaaagaacc
     4981 aggcaatgaa gatagactac agcggctaca caggtttcga ccaggtgcag aaaggtgtat
     5041 acaacgtgat ggattcgtat cagtatggcc agtatgtcaa catggcgtac tccaatagtg
     5101 gtatggatgt tcctgccggc tacaatccgg caagcccgaa atacgtagat ccgagccagg
     5161 taaacaccaa ttggtacaac gaagcgttca aaactggtat tcgtcagaat cacaacatca
     5221 atatgtcggg tggaggagct aacaacactt acaatatcgg tctcgactat tttagccaga
     5281 gaggtaccat ggaaggtgcc ggtcctaact tcgaccgttt cacagcccgt atcaacaata
     5341 caatggatgt taagtttatc aaattcaaaa ccaacatggt gtacagccac agtagtcagg
     5401 ataatatggc tcttagtaat gccaacgagt acgtacaggg cctgtatggc gcacaatatc
     5461 ctgtgatggc atctgtgctg cttttaccgc ctaccatcaa ggcttacgat ccttcaacct
     5521 gggtgctgga cgatagaatc tcagctgcaa ccaattacag ctacgactcg tacggttacg
     5581 gtacatacta tgatgatgtt catggagaca tacgtgttac gaacgttttg ctcgaaaaca
     5641 atctgattaa acgaaacaca attgttgacc gtgtagttgc ttcaggctct gcagatgttg
     5701 accttttagg tatgtttcgc ctcaagagta gcaatcacaa attgaattac aacctgaacc
     5761 tgtcatacag caaaactatg gcaaaggact ttacatttgt gcctgcattt attcagagta
     5821 caacaaacta cctggccaag agcaatgaga acctctcgga aggttatcgc agctttagcg
     5881 atgcattgat tgaaaatacg gttacctatg atggtaaatt cggtttaagt catatcaacg
     5941 ctgttgtagg tcagactttc gaacgcgaac tctatcacac actcaccggt aaaggtgtta
     6001 ccatgccgga accatactat ctgcaggtaa acaatgcaga ggaaacttcg tcaacaagct
     6061 ttgaaagcga acacgttctt gcatcgtata tcggtcgtat caattatgat ttcgatcaga
     6121 aatatctcct ttcggcaaca gcacggcgag atggatcaag ccgtctttct tcagccgacc
     6181 gctgggactg gttcccgtcg gtatcagcag gatggcgtat cgatcgtgaa aatttcttcc
     6241 ccgtatccaa atcgactatt aacctgctca aattacgtgg tagttatggt gaacttggta
     6301 acgagaacat aggcgaatat cagtatatgg atgttatgtc gagaggtaac tacacctata
     6361 gctttggtaa tactaaggtt accggttcgg ccatatccaa ttatgttaac gaagctatcc
     6421 gctgggaaaa gaaaaaaaca attgatgcag gtcttgacct tggtatgttc aacaatcagt
     6481 tggaatttac ttttgactgg tataaatcaa catccgaaga cctcctttac agtgtagcag
     6541 ttccaaccag tgcaggtgct accaatggca ctgtaactat gaacgctgca accatgcaaa
     6601 acacaggtct tgagtttctg cttgcatacc ataatcacaa acatgctgtt aagtatgata
     6661 tttcggcaaa catatcgacc atcaaaaaca aggttacaaa attgggcgtt tcaggtgaac
     6721 cgcgcacgga tggatactgt cgcacagaag taggtcgtga agtaggttcg ttctatggtt
     6781 atatttacga agggatcttc cagtcgcagg ctgagattga taaccgtgtg aactctgaag
     6841 gcaatcatat caatcagccg ggagctcaac cgggtgacgt aatttatgcc gacattgcag
     6901 gtgacttcga tgccaatggc aatccaatac ccgatggcga aatcaccaac gaagaccaga
     6961 aatatctggg aagtggtttg ccaaagataa gttatggttt gagcggacgt gcagaatgga
     7021 aaggttttga tctgagcgtt tctacctatg gagcagcagg tttcaaagcg gttgacttcg
     7081 tagatgtaac actgcacagc tcatatggtg ctctcaacaa gagtgttgat ctgttgaatg
     7141 cctggacacc ggataacacg aacacaaatg tgccccgcat agcatacaaa tcgacaggtt
     7201 caattacaaa cgatatgttc agtgagcgtt tcattcagga tgcatcctat ctgaaaattg
     7261 ccaacattga actgggctat aactttcctg acaaatggtt tggtggctat gttaacggtc
     7321 tgaggatata cgcgtcggct cagaatctgg ccacgttgtc aaaatacaaa ggctacaatg
     7381 ttgactttgc cggaggtaca ttcaccccgg gttacaacta ttgttcgtat ccaacaccac
     7441 gaacagtcat gttcggagcg cacttctcat tctaaaaaat ataatctaat taatagagta
     7501 tcaacatgaa aagtataaaa atatttctaa caatgttagc gttcattgga ggcatgactg
     7561 cctgcaatga ccaactcgat gttcagaacc caaacaatca gactacctat gattttggca
     7621 atacagagtc ggatcttaag gaagctatta tctcgtgcta caaccgcata cgcctcgaag
     7681 gaacatttgc ccgcgttggc tacacgatgg atgcggtacg tggtgacgaa gtttggaatt
     7741 catcacaaca atggtatctg gagtacgaca acctcaattc cccgggaaca atcgggatag
     7801 gagatgagtg gatatggcgc gacaactacc atgtagtaaa ccgtacaaat tttgttttgt
     7861 cgaaagttga taatgtcagc atgtcggaag cttcttacaa tcagatagcc ggacaggcac
     7921 tctttctccg ctctcttgca tattatgaac tggctaccta ctaccagaat gttccgctga
     7981 ttactgacta tgcttcttat tcggacatca acaccatgta cgcgcctaac aatactcagg
     8041 accaggtgtt tgatcagata gaggctgacc tcacaaaagc catgcagatg ttgccatcgc
     8101 gcgataaagg tggcgaatgg gcaaaagggc gtgctacaag cggtgctgct gccggatatc
     8161 tggcacgtgc actgatgttt cgtcataaat attcagaagc atatactgtt ctgaaagata
     8221 tcatcgcagg aaagtacgga cattacgaac tgacagccga ttatggtgac aatttcaggg
     8281 aaggtgctaa atatgaaaac aactcggaaa gcctcttcga agtacagttt ctggattatg
     8341 gaacaggtgg caccgacgaa gaatggacac cggtaaacat cagctcggaa gcaacacagg
     8401 gtcatgcagt ggaaagtaac tacgcttctc aggaaatggg cagctgggga gaccttgcag
     8461 cttcaccctg gttgtataac ctgttcaaaa aagaaaactg taccgatggt cgtctcgatc
     8521 cgcgtttata ctggacactg gttacattcg aaagtgaata taacaattat acaaacctga
     8581 aaactgcagc atatccgaat ggagatccgc gttgcaacac gatctatcaa aaggatatca
     8641 ctgcaactcc actttcgaac actgctcagg gtggcgtatc cattgcaaaa tttaccaatg
     8701 cgcgaaataa tatctacagt tccattacaa acggattaca ttgcggtatc aacctgcgtc
     8761 tgatgcgtta cagcgatgtt ttgctacgtg ccgcagaatg tgaaaatgaa ctccacggcc
     8821 cgacacagac agcaatcgat tatatcaaca ccgtacgtag acgtgtggct cttaaagacc
     8881 tgaaactttc cgatttcgcg tcagctgatg ctcttttcga acaaatagct aatgttgaac
     8941 gtccgaaaga attcggttgc gaaaacggtc gtggtatcga cttactccgc tggggattct
     9001 tctacgatgc aggtcgtctg aatcaactgg ttgaacatgg ttattataaa ctcgatggca
     9061 aagcctctac agaagagctt actgctgcta cagcaaccag ttcgtcgtat aagtattact
     9121 acaagggtca tgaatatttc ccgatttatc aatccactct taatgccaac ccgaacctta
     9181 caggtaactc ggcaaacaag aatgaagata acggaccagc tttcaaagct aaatggaata
     9241 tccatcctgt tgtaaaataa aatcatttcc ttaaatttgg gacttcctta ttggcatatt
     9301 tcttgtacgt taataaggaa gtctctttat taaaaaatat cagacatgag actaaagtat
     9361 ctgttaattt tatcggtcag tatcgcatcg ttattttttg caggatgcaa aaaagatgaa
     9421 gaacctaagc ctgatcctga accagttcct ggttttacca ttccaattta tgccgacgac
     9481 tattcatcca tatcatcgtg ggcaaaccgt aacaagtgga atctggccaa cgttcatgac
     9541 ccgtcggttg cgtggtacaa cggatattac tatatgtacg gtaccgatgc ctcctatggc
     9601 aacgctcacg aaggacacgg acattttcag ggcaagcgtt cgaccgatct ggttaactgg
     9661 gaatgggtag gcggaccatt ttatcaggca cccacctggg taaccgactc actcaatgcc
     9721 atccgtttac gcatgggtct cgctgccatt gcaaaagaaa acataagata cggttactgg
     9781 gcaccggttg ttcgacgggt caacgttggt ggaaaagatg tcctgcgaat gtactacagt
     9841 atcgttattg ataactatat caaaacaggc aaagccaaca ccacagccaa ctttgacgga
     9901 agctggaccg aacgggcatt tatcggtatg tgtgaatcga cagatcccgc aggagctgtt
     9961 tggactgata agggatttgt taccagttca tcatccgatc gtggcaaaaa ctacagtcgc
    10021 gccagtacaa atgactggag cgcttatttc tactacaacg ctatcgaccc tacctatatc
    10081 gtaacacccg atggaaaaca ttacctgata catggttcgt ggcatagcgg attcgcgctg
    10141 ttgcaactcg atgcaacaac aggcaagcct cttaacacgc tcggcgaacc ttatgcaaac
    10201 agtgtaagtg agctgacagc ccgttatggt gtgagaattg gaacacgtac agcttcatca
    10261 cgctggcagg gcagcgaagc acctgaaata atatacaaag atggttacta ctatttgttt
    10321 atggcttatg atggtctcga cgtaccctac aacacgcggg tagtcagaag tacaaagatt
    10381 gaaggaccat accttgacat taccggacgc aattttacca acggggcagg agactgttac
    10441 ccaatcgtaa ctcacccata caaattcaat ctgagctacg gatgggtagg tatatcacat
    10501 tgctgcatct tccagaaaga aaataccaac gaatggttct acatgtcaca gggacgttta
    10561 cctgctaatg ttggtggcaa tgcctactcc aatgccatca tgatggggca cgtacgccgc
    10621 attgtatggt gtccggcttc tgcaagcgaa cctgacaacc tgtggcctat cgcattgccc
    10681 gaacggtacg cggcagtacc cgatttcggt accataacca aagactcact tacaggtaca
    10741 tgggaacata tcaatctgaa atacagctat ggagtacaaa acagcgccac ttctctgacc
    10801 cttaaagcag atggcaccat gtcgggggca ctcacaggta gctggaatta tgatgttgca
    10861 aaaaaacagc tgaagttggg taacgttatc gtttgcgttg aacgcgaagt tgactgggaa
    10921 gcttcaccgc gctgcgtaac gtttgtatat gcaggaacgg aaaaaaatct gaacgcgact
    10981 tactggggaa agaaggtaaa ataattgatt attgaataaa aaatcataaa cagatattgg
    11041 gatgaagatc aatatttttc gttctgtaat aaatgcagtt gttttatcat cgtttattcc
    11101 gagtatgtgt attcaggcac agaaccagca tatcgtagta cacgatcctg tagctattga
    11161 atccgaagga acgtattata ttttcaatac aggaagaggc atcgcttcat tgaaatcgaa
    11221 cgatctgaaa aactggcagt ttatgaaacc cgttttcgat gtttca
//
LOCUS       DAOPYB010000098        11178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6672_length_11178_cov_9.544278, whole genome shotgun sequence.
ACCESSION   DAOPYB010000098 DAOPYB010000000
VERSION     DAOPYB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11178
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6672_length_11178_cov_9.544278"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKH58_08310"
     CDS             <1..428
                     /locus_tag="PKH58_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HNX89069.1"
                     /translation="AKPRIEVHFLGIGDAGVCARLVFQAHEGEGVAATIVDMGNRFRM
                     IVNEVKVIEPKALPKLPVACALWIPQPNLEVGAGAWIVAGGTHHSSFSFSITTEMLED
                     YAEIADLEMLIIDKDTNIRNFKQDLRNNEVYYMLNRALR"
     gene            511..2178
                     /locus_tag="PKH58_08315"
     CDS             511..2178
                     /locus_tag="PKH58_08315"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HNX89070.1"
                     /translation="MKYVIGLDYGSDSARAVIVNAETGEELASSVKYYPRWMEGKYCV
                     PTANQYRQHPQDYIDALEYTVKDALSKCPAGTAENVVGMAFDTTGSTPVFTDKNGTPL
                     AMLPEFAENPNAMFVLWKDHTAIKEAQEINDLCAKWNIDYTSYEGGIYSSEWFWAKAL
                     HVLRADEKVRSAAYSLVEHCDWLPGLLVGKTKPEEICRSRCASGHKAMWLEKWGGLPS
                     EEFLVALDPVLSGYRDRLYSDTCPSDKKTGTLTEEWAQRLGLTTNVAVAVGAFDCHMG
                     AVGAEVKPGALVRVIGTSTCDIMVSSYQEMGDKLIPGICGQVDGSVIPGMVGLEAGQS
                     AFGDVYAWFKNVVAWPVVNILSKTTLVDEATKQKLIDETVDQIIPALSVEAAKVPVSE
                     STILATDWMNGRRTPDANQKVKGTITGLNLGSSAPLIFRALVEATAYGSKAIVDRFIE
                     NGVEIKEIIGIGGIALKSPFVMQTLADVLNMPIKVAKAEQACAFGASMFASVAAGIYA
                     KVEDAQAAMGMGFASEFYPNAENHKLYNELYKNYVAVGKFTEENLFK"
     gene            2248..3387
                     /locus_tag="PKH58_08320"
     CDS             2248..3387
                     /locus_tag="PKH58_08320"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNX89071.1"
                     /translation="MKKYITGLAIIALALIGCKDNKCEKTLSGLCKTNFQTVVNGDST
                     NLFVLKNASGMEVCITNFGGRIVSIMVPDKTGKMQDVVLGFDSVADYINVPSDFGASI
                     GRYANRINQGKIVIDGDTIQLPQNNFGHCLHGGPKGWQYQVYKNVKQIDDTTIEMTRF
                     SPDGDMNFPGNVEAKVTFKLTDDNAIKIDFEATTDKKTVINMCNHSYFNLTGDATKPI
                     TESKLYINADNYTPVDSTFMTTGEIATVKGTPMDFTTPKPVGADIDKYDFIQLKNGNG
                     YDHNWVLNTAGDVSKLAAKVVSPASGITLEVYTNEPGVQVYTGNFLDGTVKGKKGILY
                     KQRTGICLETQHYPDSPNKPQWPSVILEPGKVYKSTCVYKFGVEK"
     gene            3433..5163
                     /locus_tag="PKH58_08325"
     CDS             3433..5163
                     /locus_tag="PKH58_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNX89072.1"
                     /translation="MNWTSHEFIWLDWIIIAVGILAVTWAVWHSIKKHKRSQQGADSA
                     DYLFGKGEPWYIIGAAIFAANIGSEHLVGLAGTGAKAGVGMAHWEMQGWMILILGWLF
                     VPFYQLMNNKLGKIITMPDFLKNRYTPRTGSWLSIITLIAYVLTKVSVTAFTGGIFLE
                     SLLGLPFWYGAIGLIVLTGIFTIFGGMKGVMTLSAIQTPILIIGSFLVLFLGLSALGG
                     GNIGDGWTAMIDYSKTLSNGYGTNHMFHFETGDPLYDDYPGFWVFIGASIIGLWYWAT
                     DQHIVQRVLGQHKGEASDVVMKRARRGTIAAGYFKLLPVFMFLIPGMIAAALAARPDS
                     GFTLTNPDTAFGSMVKFVLPAGVKGIVTIGFISALVASLAAFFNSCATLFTEDFYKPM
                     FKGRTEERYVLVGRIATVVVVILGIIWIPVMMSLGSLYSYLQGIQSLLAPAMVAVFVL
                     GVFSKKITPKAGEAGLIVGFLIGMTRLLTNILTNTGKNVMSGWFWDSTHWFWHTNWLI
                     FEIWLLIFIMLFMVVVSFFTKKPTAQQIEFITFTGDYKQLIKQSWNKWDVIASLGVVA
                     LCAAFYWYFW"
     gene            5232..5924
                     /locus_tag="PKH58_08330"
     CDS             5232..5924
                     /locus_tag="PKH58_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNX89073.1"
                     /translation="MTFDLNFYKSHSTFLVAVDCIIFGFHNNEIKLLVHHRAMEPEKG
                     GLSLMGGFVQKDESVEQAASNILSNLTGLENIFMEQVKVYGELHRDPGERVISCAYYA
                     LIDINAYDESLLKNHHAFWVSLDKASELIFDHSQMVKDALAILRRKAVLEPIGFNLLS
                     EKFTLTQLQSLYEAIYGKSLDKRNFRKKMQSLNFIEKLDEIDKSCSKKGAYFYKFKED
                     GNRSSEIVNYEF"
     gene            6245..7315
                     /locus_tag="PKH58_08335"
     CDS             6245..7315
                     /locus_tag="PKH58_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX89074.1"
                     /translation="MITDKTKIRIKDIAQLAGVSEGTVDRVLHNRGEVSAKSKEAVEK
                     VLQEINYSPNLLARSLASKKHYRFVYMIPEYQPNDYWESMDKGFEQAAQDFLQYNVHL
                     EKVSFNQYDVKSFKTAAGQILLSAPDAVLLAPVFTDETLIFNQELKKMEIPLSFLDSM
                     IEQADFSTYYGQNSFQSGYLAAKLLLNSLPTASKVLTITTNITGTETHQTKVRYAGFK
                     QFIAENNLNTIELINIELSSLDESTNIRLLHDTFTKHENIRAAITFNSRVYRFAYYLE
                     ALKIKDIRMIGYDLLQENIQYLKKDIISYLLAQKPEKQAYFTVRDMCSRLVLKQKVNK
                     INYMPIDIIMKENIDNYMNFKE"
     gene            7353..8600
                     /locus_tag="PKH58_08340"
     CDS             7353..8600
                     /locus_tag="PKH58_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HNX89075.1"
                     /translation="MELSKYSIGVGDRFAHQGVAQLRAIMKANQSGLEISPVWNKSNR
                     EHMYVHTHPADVRLEADTAVATLGYKGNYFVDADHINLSTVAPFTETADFFTLDVASF
                     IGKPSSEADMEAFIDSCEKYIGELCIPGIEKPLLVTKELLKSIASKFLAATEQASEIY
                     KYLVENKGAGNFITEVSMDEVESPQTPVDMLFILKMLADKGVPAQTIAPKFTGRFNKG
                     VDYRGDLEQFAREFEEDVLVIDFAVKEFGLPNELKLSVHSGSDKFSIYPIMADIIKKY
                     DKGLHLKTAGTTWLEEVIGLSVAGGEALELAKKIYANSYNRKDELCAPYADVIDIDET
                     LLPSVEEVNSWTSEKYANTLRHIPGHPDYNSNFRQLIHVAYKVAAEMGDTYTSMLVKY
                     SEIIGNCVEENIYERHLKRLFSL"
     gene            8690..9502
                     /locus_tag="PKH58_08345"
     CDS             8690..9502
                     /locus_tag="PKH58_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX89076.1"
                     /translation="MENLFSVEGKVVVITGGGGILGRGMAEYMCSQGAKVVILDRSEE
                     AGNLLLNELKEKGYEAAFFYCDVLNNEVLEQNSKDIVAKYGRIDVLVNAAGGNMPGAT
                     IGPDNTIFDLQMDAFRKVVDLNLFGTVMPSMVFGRVMVEQKKGSIINISSESALRPLT
                     RVVGYGASKAAVTNFTKYLASEMAIKFGEGIRVNAMAPGFFLTEQNRTLMTNPDGSLT
                     PRGQSIIAHTPFGRFGEPEELFGTLHWLASDASKFVTGTLTVIDGGFDVFCI"
     gene            9662..10837
                     /gene="uxuA"
                     /locus_tag="PKH58_08350"
     CDS             9662..10837
                     /gene="uxuA"
                     /locus_tag="PKH58_08350"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HNX89077.1"
                     /translation="MNKYLCKQSWRWYGPNDPVSLWDIRQAGATDIVTALHEIPNGEV
                     WPTQAILERKKIVAEAGLKWSVVESVPVHEDIKKRSGNYKQYIENYKQTIRNLAKCGI
                     YDVTYNFMPVLDWTRTDLAYQMPDRSLALRFEMAAFIAFDLYILKRPGAENDYTVDQI
                     SKAKARYDGMSESEKETLIRNIIAGLPGAEESFTIEQFQQALDTYADINEDQLRNNLF
                     YFINEICFVADKVGVRMAIHPDDPPRSIFGLPRILSTAEDVRRLFAAVPSLSNGLCLC
                     TGSFGVSSTNDLTSMLREFGERLYYVHFRSTCRNADGDFYEDNHLEGDVDMYEAMKAL
                     LVVQYTYKRNIPMRPDHGHQMIDDLHKKTNPGYSCIGMLRGMAELRGLEMGISKTLF"
     gene            10898..>11178
                     /locus_tag="PKH58_08355"
     CDS             10898..>11178
                     /locus_tag="PKH58_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNX89078.1"
                     /translation="MSLELKKDCKYSLLVATSMGVRITPQNGQPVHCSKLFEMQATSA
                     ETNVASVSSFLGLPVKVLTTFVKDSPIAQFIKSDLRSRNMDFEGPEVAQG"
BASE COUNT         3443 a         2120 c         2401 g         3214 t
ORIGIN      
        1 aagcaaaacc acgtatcgaa gttcatttct taggaattgg tgatgcaggt gtttgtgcac
       61 gtttggtatt ccaggctcac gaaggtgaag gtgttgctgc aactattgtg gatatgggca
      121 accgtttccg catgattgta aacgaagtaa aagtgattga acctaaggca ttgcctaaat
      181 tgccagttgc ttgtgcattg tggattccac aaccaaacct cgaagtgggt gcaggtgcat
      241 ggattgttgc cggtggaact caccattcaa gcttctcatt ctctatcact acagagatgt
      301 tggaagacta tgcagaaatc gccgatttgg aaatgttgat tatcgacaaa gacactaaca
      361 tccgcaattt caaacaggat cttcggaata acgaagtata ttatatgctt aacagagcat
      421 tgagataaat aataaatgat ccgatgtgct aatgttccaa ttctggatac attagcacat
      481 tgacatattt aaaatcagca catttatatt atgaaatacg ttattggatt agactatgga
      541 agcgactcgg cacgtgctgt gatcgttaat gccgaaaccg gtgaagagct ggcatcttca
      601 gtaaaatatt atccgcgctg gatggaagga aagtactgtg taccaacagc caaccagtac
      661 cgtcagcatc cgcaggatta cattgacgcc cttgaatata cagtaaagga tgccttgtcg
      721 aaatgtcctg ccggaacggc cgagaacgtg gtaggaatgg catttgacac caccggaagt
      781 acaccggtat tcactgacaa aaacggaact ccgcttgcga tgcttcccga atttgcagaa
      841 aatccgaacg ccatgttcgt attgtggaaa gatcatactg ccattaaaga agctcaggaa
      901 atcaacgacc tttgcgctaa atggaatatt gattacactt cgtacgaagg tggcatttat
      961 tcatcggaat ggttctgggc aaaagcgttg cacgtgttgc gtgccgacga aaaagtgcgt
     1021 agtgctgcct attcgctggt agagcactgc gactggttgc ctggccttct ggttggtaaa
     1081 accaaaccgg aagaaatctg ccgcagccgc tgtgcttcgg ggcacaaagc catgtggctc
     1141 gaaaaatggg gcggtctgcc atcggaagaa tttcttgttg cacttgatcc tgtattgagc
     1201 ggttatcgcg acagacttta cagcgacact tgtccaagtg acaaaaaaac cggaactctt
     1261 accgaagaat gggcacaacg tctgggtctg acaacaaatg tagctgttgc tgttggcgct
     1321 ttcgactgtc acatgggagc cgttggagca gaagtgaaac cgggagcact cgtacgcgtt
     1381 atcggaactt ctacctgcga catcatggtt tcaagctatc aggaaatggg cgataaactt
     1441 attccgggta tctgtgggca ggttgacgga tcggttatcc ccggaatggt tggtctggaa
     1501 gccggacagt cggcttttgg tgatgtatat gcctggttta aaaacgtagt agcctggccg
     1561 gtagtgaata ttctttcgaa aacaacactt gttgacgaag ccaccaaaca aaaacttatt
     1621 gacgaaaccg ttgatcagat tattccggct ctttctgtag aagctgccaa ggttccggta
     1681 tcggaatcta ctattctggc taccgactgg atgaatggtc gccgtacacc ggatgcaaac
     1741 cagaaagtga aaggaacaat caccggtctg aatttgggaa gttcagctcc gcttattttc
     1801 cgtgcattgg ttgaagcaac tgcttacggt tcgaaagcca ttgttgatcg ttttatcgaa
     1861 aatggtgttg agattaaaga aatcatcggt atcggtggta ttgctttgaa atcgcctttc
     1921 gtaatgcaaa cgctggctga cgtattgaac atgcctataa aagtagcaaa agctgaacag
     1981 gcatgtgctt ttggcgcttc aatgtttgcc tcggttgccg ctggcattta tgcaaaggtt
     2041 gaagatgcac aggctgcaat gggtatggga tttgcttcgg aattctatcc gaatgccgaa
     2101 aaccataaac tctacaacga gttgtacaaa aattacgttg cagtaggtaa atttacagaa
     2161 gaaaatttat tcaaataata aaaaagactc cgattcgttt caaaacggat tggagtcttt
     2221 ccataacatt aaataatacc actacacatg aaaaagtata taactggtct tgcaattatt
     2281 gctctggctc tgatcggatg caaggataat aaatgcgaaa aaacactttc cggcttgtgt
     2341 aaaaccaact ttcaaaccgt ggtaaacgga gattcaacca atctgtttgt attgaaaaat
     2401 gcaagcggaa tggaagtatg catcaccaac ttcggtggac gtattgtatc tatcatggta
     2461 cctgacaaaa caggtaagat gcaggatgta gttcttggct tcgattctgt agccgactac
     2521 atcaatgttc cgagtgattt tggcgcttcg attggacgat atgccaaccg cattaatcag
     2581 ggtaaaatag tgattgatgg cgataccatt cagttgccac aaaacaactt cgggcattgc
     2641 cttcatggcg gaccgaaagg ctggcaatat caggtttaca aaaacgtgaa acaaattgac
     2701 gacaccacta tcgaaatgac ccgtttttcc cccgatggag atatgaattt ccccggtaat
     2761 gttgaagcaa aagtaacttt caaactgacc gatgacaacg cgattaaaat tgattttgaa
     2821 gctactaccg ataaaaaaac agtgattaac atgtgtaacc actcatattt caacctcaca
     2881 ggcgatgcta ctaaaccgat taccgaaagc aaactgtaca tcaatgccga caattacact
     2941 ccggttgaca gtacgtttat gactaccggc gaaatagcca cagttaaggg caccccgatg
     3001 gatttcacta ctcccaaacc ggtaggtgca gacattgaca aatacgactt tattcagctt
     3061 aaaaacggta atggttacga tcataactgg gtattgaata ctgccggtga tgtatcaaaa
     3121 ctggcagcaa aggttgtttc acctgcaagc ggaattaccc ttgaagttta caccaacgag
     3181 ccgggcgttc aggtttatac gggtaacttc ctcgatggta ctgtaaaagg caaaaaaggg
     3241 attctttata aacaacgtac gggaatttgc ctcgaaactc aacattatcc cgatagtccc
     3301 aacaaaccac agtggccttc ggttattctc gagcccggaa aggtgtataa gagcacatgt
     3361 gtttataagt ttggtgttga aaaataaaaa cggaaagaaa taatatcaga aaataattat
     3421 aaataaaaaa ttatgaattg gacctcacat gaatttattt ggctcgactg gataataata
     3481 gcagtcggaa ttcttgcagt tacctgggct gtatggcatt cgataaagaa acacaaacgt
     3541 tcgcaacaag gggcagacag tgctgactac ctgtttggta aaggtgaacc ctggtatatt
     3601 attggagcag ctatctttgc tgccaatatt ggttcggaac accttgtagg gcttgccgga
     3661 accggagcaa aagcgggtgt tggtatggca cactgggaaa tgcaaggctg gatgattctc
     3721 atcctgggct ggcttttcgt acctttctat caattaatga ataataaatt gggcaaaatc
     3781 attaccatgc ccgatttcct gaaaaaccgc tacaccccgc gtaccggttc gtggctgtcg
     3841 atcataacac tgatagctta cgtgctgacc aaagtgagtg taactgcttt taccggtggt
     3901 attttcctcg aaagtcttct gggattacct ttctggtatg gagctattgg cttgattgtg
     3961 ttaacaggaa tttttaccat tttcggagga atgaagggag taatgacact ttctgcaata
     4021 cagacaccaa ttcttattat tggctcattt ctggttcttt ttctgggact ttcagctctt
     4081 ggtggcggaa atatcggcga cggctggact gctatgattg attattcaaa aacactgagc
     4141 aacggctatg gaactaatca tatgttccac ttcgaaacgg gtgacccgct ttacgatgac
     4201 tatcccggat tttgggtatt tatcggagct tcgatcattg gtctttggta ttgggctacc
     4261 gaccagcaca ttgttcagcg tgtactcggt cagcacaaag gcgaagcaag cgatgtagta
     4321 atgaaacgtg cacgcagagg aaccattgct gcaggttact ttaaattgtt acctgtattt
     4381 atgttcctga taccgggtat gatagctgct gcactggcag ctcgtcccga cagtggattt
     4441 acacttacca accccgacac cgcatttggt tccatggtta agtttgtatt acctgccggt
     4501 gtaaaaggta ttgtaaccat tggttttatc tctgcactgg ttgcttccct tgcagcattc
     4561 ttcaattcat gtgctacact gtttaccgaa gacttctata aacccatgtt caaaggcaga
     4621 accgaagaaa gatatgtact ggttggacgt attgctacag tcgtagtggt gatacttggc
     4681 attatctgga taccggtcat gatgagtctc ggcagtcttt actcctacct gcaaggcatt
     4741 cagtcgttgc ttgctccggc tatggtagca gtattcgtac tgggagtatt ctccaaaaag
     4801 atcactccaa aagctggtga agctggctta attgttggct ttttgattgg tatgacacgt
     4861 ttgctgacaa acatcctgac aaatacaggt aaaaatgtaa tgtcaggctg gttctgggat
     4921 tctactcatt ggttctggca taccaactgg ctgattttcg aaatctggtt gctaatattt
     4981 attatgctgt ttatggttgt agtatcattc tttactaaaa aaccaactgc tcaacaaatt
     5041 gaattcatca cattcacagg tgactataag caacttatca aacagagctg gaacaaatgg
     5101 gatgttattg cttctcttgg tgttgtagca ctctgcgctg ctttctactg gtatttctgg
     5161 taaaaaaaat gaatataaaa gtaaactttg aataaaatac attcaaagtt tactttacaa
     5221 tttttacttc aatgacattt gatttaaatt tttacaaatc tcacagtaca tttttggtcg
     5281 cagttgactg tataatcttt ggttttcaca ataacgaaat aaaactactg gtgcatcatc
     5341 gcgctatgga acctgagaaa ggtggtttgt cgctcatggg aggttttgtt caaaaagacg
     5401 aatcggtaga acaggcagca tcaaatatac tttcgaacct taccgggctg gagaatattt
     5461 ttatggagca ggtgaaggtt tatggagaac tccatcgcga tccgggtgag cgggtaattt
     5521 cctgtgcata ttatgccttg attgacatta atgcctatga tgaatctctg ttgaaaaatc
     5581 atcatgcttt ttgggtaagc ctggacaagg cttccgaatt gatttttgac cattcacaaa
     5641 tggtaaaaga tgcgctcgct attcttagaa gaaaagcggt tcttgaacca atcggtttca
     5701 atcttctatc cgaaaaattc acccttactc agctacaatc tctttatgag gcaatttacg
     5761 gcaaatcact ggataaacgt aacttccgaa aaaagatgca aagccttaat ttcatcgaga
     5821 aattggatga aattgacaaa tcgtgttcaa aaaaaggagc atacttttat aaattcaaag
     5881 aggacgggaa cagaagttct gaaattgtaa attatgagtt ttaatcgttg attatacaat
     5941 attatatcat tagtataatc acatgttatt tcagacattt aagttaatat cacccctgat
     6001 aatcttaaaa tcgaaaacat aaacatgttt gaaaacataa atttcggaat tttcaaattt
     6061 ttcaattaaa acaccaccat atattttcct gatttactta cagttacaca tccgaacaga
     6121 tagttataac aaaattcgtg tccgcacacg ttttttgtct aaattatttg ttctttcaat
     6181 attaagttct atttttgcat caaatttaaa tcgtgcccgt acacgaatat gcaaatattg
     6241 aattatgatc acagacaaaa caaaaataag aataaaagac attgcacaat tagcaggtgt
     6301 ctcagaaggt actgttgatc gtgttctgca taatcgcggt gaagtttcgg cgaaaagcaa
     6361 agaagcagtt gagaaagtac tgcaagagat taactactct cctaacctgc ttgcccgttc
     6421 gctggcctcc aaaaaacact accgttttgt ttacatgatt cctgaatatc agcctaatga
     6481 ttactgggaa agcatggaca aaggatttga gcaggcagca caggattttc ttcaatacaa
     6541 tgttcatctg gagaaagtat cttttaatca gtatgatgtt aaatcattta aaacagcggc
     6601 tggacagatt ctcctatcag cacctgatgc tgtattgctt gcaccggtat ttacagatga
     6661 aacattaatt tttaatcagg aactaaaaaa aatggagata cccctttctt ttttagattc
     6721 aatgatcgaa caagcggatt tttctactta ttacggacaa aactcctttc aaagcggata
     6781 tctggcggca aaattattgt tgaactcatt accaactgca tcaaaagtat tgacaataac
     6841 gacaaacatt accggaacag aaactcacca gacaaaagtt cgttatgctg gatttaaaca
     6901 atttatcgct gaaaacaatt taaataccat agaacttata aatattgaat tatccagtct
     6961 ggatgaaagc actaatataa gattactgca tgacacgttt acaaaacacg aaaacatcag
     7021 agcagcaatt actttcaatt caagggttta ccggtttgct tactatctgg aagctttaaa
     7081 aataaaagac atccgtatga ttggatatga tttgctacag gagaatattc agtacctgaa
     7141 aaaggacata atatcctacc tgctggctca aaaacctgag aaacaagctt attttacagt
     7201 acgtgacatg tgttccagat tggtactcaa acagaaagtt aataaaatca attatatgcc
     7261 gattgatatt attatgaaag aaaatatcga caattatatg aattttaagg aataaaacca
     7321 tatatatatc aatcaattaa aaattctaaa tcatggaatt aagcaaatac agtattggtg
     7381 ttggcgatcg tttcgctcat cagggagttg cccaattacg ggctataatg aaagccaatc
     7441 agtcgggact ggaaatcagc ccggtatgga ataaatcgaa ccgcgagcac atgtatgtac
     7501 acacccatcc ggctgatgta cgtctagaag ccgatacagc agttgccacg cttggttata
     7561 agggaaatta ctttgtagat gcagatcata tcaatctgag tacagttgct ccgtttacgg
     7621 aaactgcaga ttttttcaca ctcgatgttg cttcttttat tggtaaacca agttcagaag
     7681 ctgatatgga agcatttatt gattcatgtg agaagtatat tggtgaactt tgcattcccg
     7741 ggattgaaaa accactgctt gttaccaaag aattactgaa aagtattgct tccaaatttt
     7801 tggcagctac cgagcaagct tctgagatat acaaatatct tgttgaaaat aaaggtgctg
     7861 gcaattttat aacggaagtt tcgatggacg aggtggaaag cccgcaaaca cccgttgaca
     7921 tgcttttcat tctcaaaatg cttgcagata aaggtgtacc ggctcagacg attgcaccaa
     7981 agtttaccgg aagatttaac aaaggggttg attaccgcgg agatttagaa cagtttgctc
     8041 gtgaatttga agaagatgta ctcgtaattg attttgctgt taaagaattc gggcttccca
     8101 atgagctgaa actgagcgtt cattcgggca gcgacaagtt ctccatctac ccaattatgg
     8161 cagatattat caaaaagtac gataaaggtc tgcatctgaa aacagccgga actacctggc
     8221 tcgaagaagt aatcggtttg tccgtagcag gtggtgaagc actggaactg gcaaagaaaa
     8281 tttacgcaaa ctcctacaat cgtaaggatg aattgtgtgc tccttatgcc gatgttattg
     8341 atattgacga aactctgctg ccttctgtag aagaagtgaa cagctggaca agtgaaaaat
     8401 atgctaatac cttacggcat attccggggc atcccgacta taattccaat ttcaggcagt
     8461 taattcatgt tgcctacaaa gtggcagccg aaatgggcga tacctacact tccatgctgg
     8521 taaaatattc ggaaataatt ggaaattgcg tggaagaaaa catatatgaa cgtcatttga
     8581 aacgtttatt tagcttataa taaaatttcc caaattgctt agcataagaa aactatggaa
     8641 ttaaggagtt tgttttaatg aaaacgggta aaatttaata aataaaaata tggaaaatct
     8701 ttttagtgta gaaggtaaag ttgtagttat caccggtggt ggtggtattt taggtcgtgg
     8761 tatggctgaa tatatgtgca gccagggagc aaaagttgtt attcttgacc gaagtgaaga
     8821 agcggggaat ctgcttttaa atgaacttaa agaaaaaggg tacgaagcag cctttttcta
     8881 ttgcgatgtt cttaacaatg aagtacttga acaaaattcg aaagatattg tagctaaata
     8941 tgggcgcatt gatgttcttg taaatgctgc cggtggaaat atgccgggtg ctaccattgg
     9001 ccctgataat actatctttg atctgcaaat ggatgctttc cgcaaagtag tggatttgaa
     9061 cctctttggc actgtaatgc caagcatggt ttttggtaga gtaatggtcg aacagaaaaa
     9121 aggaagtatc atcaatattt catcagagtc tgcattacgt ccgctcaccc gtgtagtagg
     9181 ttatggtgca tcaaaagcag ctgttaccaa ttttaccaaa tacctggcat ccgaaatggc
     9241 tatcaaattt ggcgagggaa ttcgtgtgaa tgcgatggcg cctggatttt tcctgaccga
     9301 gcaaaaccgt acattaatga caaatccgga tgggtcgctc acccctagag gtcagtcgat
     9361 tattgcacac actccttttg gccgttttgg tgaacccgag gaattattcg gaacccttca
     9421 ctggcttgcc agcgatgcat ccaaattcgt aaccggaact cttaccgtta ttgacggcgg
     9481 ttttgatgta ttttgcattt aacatttaat tttcgtccgg agttgatatt ctcatcggtt
     9541 aactttcatc tccggacttt ttgatattta aggataagtt ataaaaaaga tttaattcaa
     9601 tttgtcgtat ttagttgctt aataaatatg caattaaata cgatcattaa aaaactaagg
     9661 tatgaataaa tatttatgta aacaatcatg gagatggtat ggacctaatg acccggtatc
     9721 gctatgggat atacgtcagg ctggtgcaac tgacatagta accgcactgc atgaaattcc
     9781 aaatggtgaa gtttggccta cacaagccat tctggagaga aaaaagattg tcgcagaagc
     9841 aggattgaaa tggtctgtgg tagaaagtgt accggtacac gaggatatca aaaaaaggtc
     9901 cggtaattat aaacagtata ttgagaatta taagcaaaca atacgaaatc tggcaaaatg
     9961 cggaatttat gatgtaactt ataactttat gccggttctt gactggacac gaacagatct
    10021 ggcttatcag atgccggaca gatcattggc actgagattt gaaatggctg cttttattgc
    10081 atttgacctt tatattctta aacgtccggg agccgaaaat gattatactg tggatcagat
    10141 atcgaaagca aaagccaggt atgacggcat gagtgaatca gaaaaagaaa ctttaatcag
    10201 aaatattatt gcaggattgc ccggcgctga ggaaagtttt acaatcgaac aatttcaaca
    10261 agcattagat acgtatgcag atatcaacga agatcagttg cgcaacaacc ttttctattt
    10321 tattaacgag atatgcttcg tagctgataa agtaggggta agaatggcta tccatccgga
    10381 cgatcctccc cgctcaatat ttggcttgcc cagaatttta agtacagccg aagatgtgcg
    10441 tagattattt gctgctgttc caagcttatc gaacgggtta tgtttatgca ccggttcttt
    10501 tggtgtttca tcgaccaacg acctgacctc catgttgcgt gaatttggtg aacgtcttta
    10561 ttacgtgcat ttccggagta catgccgcaa tgccgacggt gatttttatg aagacaatca
    10621 tcttgaagga gatgtggata tgtatgaagc aatgaaagct cttttagttg tgcaatatac
    10681 ttataaaaga aacattccta tgagaccgga ccatggacat cagatgatcg acgaccttca
    10741 taaaaaaacc aatccgggtt attcatgtat aggcatgttg cgtggtatgg ctgaactacg
    10801 cggactggaa atgggaattt caaaaacact tttttagatt aaatagaaaa acactgtaaa
    10861 ttttcgagat attataaacc ttcaaaaatc aaatatcatg agtttagaac taaaaaaaga
    10921 ctgtaaatat tctttgctcg ttgccaccag tatgggtgtt cgcattactc cgcaaaacgg
    10981 gcagccggtt cattgcagta aattgtttga aatgcaggct acgagtgccg aaaccaatgt
    11041 tgcaagtgtt tcttcttttt taggacttcc agtaaaagtt ttaaccactt ttgtaaaaga
    11101 cagcccgatt gcacaattca ttaaaagcga tttgcgcagc cgcaacatgg atttcgaagg
    11161 tccggaggtt gcacaggg
//
LOCUS       DAOPYB010000099        11154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6702_length_11154_cov_9.663573, whole genome shotgun sequence.
ACCESSION   DAOPYB010000099 DAOPYB010000000
VERSION     DAOPYB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6702_length_11154_cov_9.663573"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="dprA"
                     /locus_tag="PKH58_08360"
     CDS             complement(<1..842)
                     /gene="dprA"
                     /locus_tag="PKH58_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNX89079.1"
                     /translation="MMDTDSLLQYQIGVTLIKGIGPNLAKNLIAYIGSVEGVFKERKQ
                     NLAKIPGIGEVLSHEIVSQNVMQRAEKEVEFIRKNGIQTMFYTEKSYPYRLKECADAP
                     VLLYFKGDHNLNSSKFIAVVGTRKATEVGKEICKCIIQDIAFSQPNATIVSGLAYGVD
                     ICAHKAAIDYNMATIGVLGHGLDRIYPAIHRPAAVKMVQHGGLLTEYISDTNPDRQNF
                     VQRNRIIAGLCDALIVIESGRSGGALITAELANDYNRDVFAVPGRVTDEWSAGCNKLI
                     RENKA"
     gene            complement(839..1210)
                     /locus_tag="PKH58_08365"
     CDS             complement(839..1210)
                     /locus_tag="PKH58_08365"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNX89080.1"
                     /translation="MKFISAYWRSLIMTLIILFLSVYPFTSDMGLPRFQYRDKFIHSL
                     MYVALAFVMYYDYRKTNQSVEKFRHFLPVLFLFPLVFGGLIEIVQAVFFPPRTAEWLD
                     WFSDIAGSVIGFLIAKMIFRK"
     gene            1377..2168
                     /locus_tag="PKH58_08370"
     CDS             1377..2168
                     /locus_tag="PKH58_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX89081.1"
                     /translation="MKKILPLMLFAVIFTACSSVMLTGRKQLNLVSDAEINAMSFQSY
                     KQFIDSVPLSTNKTNTAMVKRVATNIANAVETYMKANGYEADIANFAWEINLVKDTTV
                     NAFCMPGGKMVAFEGILPKTLTETGMAVVIGHEIAHAVARHSNERLSQQMMVQYGGAL
                     TGVLLSGKSAAVQQGIGALYGIGSQLGVLLPYSRKQEYEADKLGLIFMAMAGYNPNEA
                     ITFWQRMTADKSGSVSDFMSTHPSDEKRIARLQEEMPGAMTYYKK"
     gene            complement(2234..2884)
                     /gene="upp"
                     /locus_tag="PKH58_08375"
     CDS             complement(2234..2884)
                     /gene="upp"
                     /locus_tag="PKH58_08375"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNX89082.1"
                     /translation="MKIINLSENNSVLQHYLREIRSVDIQKDSMRFRRNMERIGEIMA
                     YEISKTMNYKPDKVKTPLGIKELTKIEEKIVISTILRAGLPFHNGFLSYFDSAENAFV
                     SAYRKYKDALKFDIFIEYIASPKLDGKTLIITDPMLATGGSMELAYGALQTKGKPRKV
                     IIATIIASQPAIDYMKEKFSDDITLWTADIDPELNEHSYIIPGLGDAGDLAFGEKL"
     gene            complement(3035..4690)
                     /locus_tag="PKH58_08380"
     CDS             complement(3035..4690)
                     /locus_tag="PKH58_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX89083.1"
                     /translation="MIEKYLQQTEFSDFDDFKANYKLIVPEQFNFGYDIVDEWAALQP
                     DKKALLWTNDKGDCHEFTFAEMKKYTDQTASYFQTLGLKKGDIVMAILKRRYEFWFTI
                     IALHKLGITIIPATHLLTKKDIVYRNNAADITAIIAVGEEPIASAISEALPESPSVKQ
                     LISVGPIIPEGWQDFHEGIKNAPEFTPISHVNSNDDPMIISFTSGTTGNPKMVTLDFV
                     YPLAHIITAKYWHNLDENSLHLTIADTGWLKAVWGKLYGQWIVGACVFVYDHDKFAPA
                     DMLKMIEKHRVTSLCAPPTIFRFLIREDLTKYDTSSLKYCTIAGEALNPEVYKQFYKL
                     TGIKLREAYGQSETTVSVLTAPWVEPKPGSMGLPSPHYNVDLLTPDGRSAEVGEQGQI
                     VIRTDKGYPVGLFKGYHRNQQLTDECYQDNIYYTGDVAWRDEDGYFWFVGRADDVIKS
                     SGYRIGPFEVESALMTHPAVVECAITGVPDEIRGQVVKATIILSKEYRDKAGDELIKE
                     IQDHVKSVTAPYKYPRIIEFVDELPKTISGKIRRKEIRDSDAK"
     gene            complement(4720..5268)
                     /locus_tag="PKH58_08385"
     CDS             complement(4720..5268)
                     /locus_tag="PKH58_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX89084.1"
                     /translation="MNYQIKQIAERLRGLREALELTTEEFALKCEISKQDYELWESGD
                     ADIPMSFIFHVAQTFGLDTTELLSGETAHASSFYVTRKGKGISVERTKAYKYQALGAG
                     FFGNRAELFEVTVEPNDEPMTLNTHTGQEFNFVLQGTMQLHIAGNDLVLEEGDSIYFD
                     ASKQHGMKALNNEKVVFLAMIV"
     gene            complement(5284..5898)
                     /locus_tag="PKH58_08390"
     CDS             complement(5284..5898)
                     /locus_tag="PKH58_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89085.1"
                     /translation="MRTFLKIISYIFQPLLMPFYGMILLMQTNLFSIFSPAYKIIAVA
                     GTLLFTGLLPALPIFMMMRRGQIKDLFISKREERTMPYLFSMLSYVFWLFFLWRTLQF
                     PPEFSLVAAGSVLSVFLMVFINLKWKISAHLAGMGGFLGGVFAYSYLMAVNPVGLIVV
                     SVIITGFVAVSRVYLKAHTLSQTLAGFSLGFLCVFLPGVIYQLI"
     gene            complement(5919..7367)
                     /gene="rpoN"
                     /locus_tag="PKH58_08395"
     CDS             complement(5919..7367)
                     /gene="rpoN"
                     /locus_tag="PKH58_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNX89086.1"
                     /translation="MLKQQLQQKLQQRLSPAQIQVIKMLEIPTVELEERIRQELEENP
                     ALEEGVEKTENSDEEEDSNDEYQADDNVDFEEYMPDDDIPDYKLMVNNSSGDNKHEEI
                     PFSAGLTFHEFLSEQVGLLQLTSHERNLMEYIIGNIDDEGYLRRSPESMVDDIVFQAG
                     IQTNDDEMKRLIREVQQFDPAGVGAEDLHECLLLQLERREPNQDVMNAKRIITGYFDE
                     FSRKHYEKIIRSMNITEEELKDALTQIIRLNPKPGNAWGGNVLEKSLSTIVPDFLVEN
                     NEGTLTVQLNNSNIPDLRVNSTYHDMFRDYSSNKENQNKEMKDAVMFAKQKVDAARWF
                     IDAIKQRQQTLITTMTAIVNFQREFFIEGDETYLKPMVLKEIADMTGYDISTISRVSN
                     SKYVQTEFGIFPVKFFFSETMINDSGEEISTREIKKIMLECIDEEDKRDPLTDEKLVE
                     LLKEKGYIIARRTVAKYRELLNIPVARLRIEI"
     gene            7642..8067
                     /locus_tag="PKH58_08400"
     CDS             7642..8067
                     /locus_tag="PKH58_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HNX89087.1"
                     /translation="MKKDFWEGFGIYCSNHPVLGKRKSKFMLYNTKMKGVELKFDANR
                     EGAFVILELNNPKEEDRLKRYEQFEKYKAVMEENFPEGLIWDFAYKLETGHEVCRIYS
                     QKTGIDIHKRIHWMEFYTFMASEMLKLEKAFRYVKEAVE"
     gene            8139..>8848
                     /locus_tag="PKH58_08405"
     CDS             8139..>8848
                     /locus_tag="PKH58_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HNX89088.1"
                     /translation="MIDQLTIDRINEAAHIEEVVSDYVTLRRRGVNMIGLCPFHNEKT
                     PSFTVSPAKGIFKCFGCGEGGNSVHFIMKQEQLSYYEALKHLARKYHIEVRERELSAE
                     ELAARNDRESMLLVNDFAQKYFANILHHHVDGKSIGLGYFRERGFRNDIIEKFQLGYC
                     LDEKDAFSKSAIKAGYNKDFLVKTGLTIEGDNNYLADRFRGRVMFPVHSLSGKVLAFG
                     GRVLKKDEKTAKYVNSPE"
     assembly_gap    8849..8948
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8949..9263
                     /locus_tag="PKH58_08410"
     CDS             <8949..9263
                     /locus_tag="PKH58_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNX89089.1"
                     /translation="HPDIEISKFTADLILEKYTLSKIHSKFHKLETEVDRLSELAPRF
                     IFEYKLAIIMDKRKQKLMEMKTAAEARNDKMMQQIMREIEAIDNFKNKLSKELDRVLN
                     LL"
     gene            9323..10576
                     /locus_tag="PKH58_08415"
     CDS             9323..10576
                     /locus_tag="PKH58_08415"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNX89090.1"
                     /translation="MKTIENTWQKTVVKASRSLNLISEEKINETLLALADAAVAATGV
                     ILTANQLDLAVMETSNPMYDRLMLSEERIRAIADDIRNVAALPSPLGKTLMETTRPNG
                     MNIRKISVPFGVIGIIYEARPNVSFDVFSLCFKSGNACVLKGGSDAYNSNLAITHLIK
                     NILRQHGLDENTVTLLPAGHEAAAEMMNATGIVDVIIPRGGKGLLDFVRKNSQVPVIE
                     TGAGIVHTYFDEDGNLEKGKEIIFNSKTRRVSVCNTLDCLIIHEKRLPDLPALCEKLA
                     EKNVIIYADSAAYGELKGVYPVTLLQKASEESYGIEFLDYKMAVKTVADIREAVEHIS
                     THSSRHSECIITENRTTAEHFTKVVDAACVYTNVSTAFTDGAQFGLGAEIGISTQKLH
                     ARGPMGLEELCSYKWIVSGSGQTRS"
     gene            10872..11117
                     /locus_tag="PKH58_08420"
     CDS             10872..11117
                     /locus_tag="PKH58_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNX89091.1"
                     /translation="MNIYIGNLSYKVRESDLQGVIGEYGEVSSCKIIKDRETGKSKGF
                     GFVEMTDDAAAAKAIEELNGAELDGRTLVVKEARPRA"
BASE COUNT         3239 a         2320 c         2273 g         3222 t
ORIGIN      
        1 gctttatttt cacggatgag tttgttgcat ccggccgacc actcatcggt cactctgcca
       61 ggcacagcaa atacatcccg gttgtaatcg ttggcaagtt cagcagtaat cagagcacct
      121 ccgctcctac ccgattcaat gacaataaga gcatcgcaca gcccggcaat gatccggttt
      181 cgctgaacga aattctgcct gtcgggattt gtatcactta tgtactcggt aagcaatcct
      241 ccgtgctgca ccatctttac agcagccggg cgatgaatag ccggatatat tctgtcaaga
      301 ccatgcccca aaacgccaat agtggccata ttataatcaa tcgcagcttt gtgggcacat
      361 atatccactc cgtaagcaag accgcttaca atcgtcgcat taggctgtga gaaggctata
      421 tcctgtatga tacatttgca gatttcctta ccgacttcag tagctttacg ggtacctaca
      481 acagcaataa attttgaaga gttcagatta tgatcacctt tgaaataaag caggaccggt
      541 gcatcggcac attccttcaa ccgataggga tacgattttt cggtataaaa cattgtttga
      601 attccgttct tccggataaa ttccacttcc ttttcagccc tttgcattac attctgtgaa
      661 acaatttcgt gtgacaacac ctctccaatc ccgggtattt ttgccagatt ttgttttctt
      721 tccttgaaaa caccttcaac tgacccgata taagctatca gattctttgc aagattggga
      781 cctatacctt tgatgagagt aactcctatc tgatattgca ataaactgtc tgtgtccatc
      841 atttccggaa aatcattttt gctattaaga atccaattac cgaaccggca atatccgaaa
      901 accaatcgag ccattcggca gtacggggcg gaaaaaacac agcctgaact atctcgatga
      961 ggccaccaaa cacgagcggg aaaaggaaca gtacaggtaa aaaatgtctg aatttttcaa
     1021 ccgactgatt tgttttgcga taatcgtaat acatcacaaa tgccaatgct acatacataa
     1081 gtgagtgaat aaatttatcg cggtactgaa accggggaag tcccatatct gatgtaaagg
     1141 gatatactga caggaaaaga ataatcagcg tcataataag agaacgccaa tatgctgaaa
     1201 tgaatttcat aatcatcagt tgtattttat taggtcacga tgaaatatcg ttttttaggc
     1261 acaatttttg taaataaaat ttcttaggca atgaatatta ttttatacct ttttgttaga
     1321 tttgcaaaga taatttttta agataaatat attgttgaaa ttaaaatttt ttgattatga
     1381 aaaaaatcct tccgttgatg ctgtttgcag taatatttac agcttgctcc agtgtcatgc
     1441 ttaccggacg caagcaactg aatttggtct ccgatgcaga aattaatgca atgagttttc
     1501 agagttataa acaattcatt gactctgtgc cactttcgac caataaaacg aacacagcca
     1561 tggtaaaaag agtagcaaca aacattgcta atgcagttga aacttacatg aaggcaaacg
     1621 gttacgaagc cgatattgca aacttcgcct gggaaataaa tctcgttaaa gacacaacag
     1681 taaacgcatt ttgtatgccg ggtggaaaga tggtagcttt tgaaggtatt ttaccaaaaa
     1741 cacttacaga aacaggtatg gcagtagtaa tcggtcatga aattgctcat gcagtggcac
     1801 gtcacagcaa tgaacgtctg agccagcaga tgatggttca atacggcggc gcactgacag
     1861 gtgtacttct ttcgggcaaa tcggcagcgg tacaacaggg catcggcgcg ttgtatggaa
     1921 ttggttcgca acttggcgta ttactcccct actcccgcaa acaggaatat gaagccgata
     1981 aactgggttt aatctttatg gctatggctg gatataatcc taacgaggct atcacattct
     2041 ggcagcgtat gactgctgat aagtccggtt ctgtttctga cttcatgagt actcacccat
     2101 ccgacgaaaa aagaattgca cgtctgcagg aagaaatgcc gggcgcaatg acttattata
     2161 aaaaataaca cacaaaacag gcaaaaaaaa caggcaaccg aatattcagt tgcctgtttt
     2221 tttatgaatt gaattataac ttttcaccga aagccaggtc gcctgcatcg ccaagccccg
     2281 gtatgatata cgaatgttcg ttcagttcgg gatcaatatc ggcagtccag agtgtaatat
     2341 catccgaaaa tttttctttc atataatcga tagccggctg actggcaata attgtagcga
     2401 taatcacttt acggggcttg cctttggttt gaagggcacc gtacgcaagt tccatggaac
     2461 cgccggttgc cagcatggga tctgttataa tcagggtttt accatccaat ttcggagaag
     2521 caatatattc aatgaaaata tcgaatttaa gcgcatcctt gtacttccgg taagccgaaa
     2581 caaatgcatt ttcggcactg tcgaaatagc tcagaaatcc attgtgaaaa ggaagtccgg
     2641 cacgaagaat ggtggaaata acaatttttt cttcaatttt ggttaattct ttaattccaa
     2701 gcggagtctt tactttgtcc ggtttgtaat tcatcgtttt gcttatttca tacgccatta
     2761 tttcaccgat ccgttccata ttgcggcgaa aacgcatgga gtctttctgt atatccaccg
     2821 agcgtatttc cctcagatag tgttgcagaa ctgagttgtt ttctgataaa ttgatgattt
     2881 tcatattttt ggaattattt aaccgtatat ttattattga attttgactt gaaattgaat
     2941 ggcgcaagaa aaatccgaca aaagtagaaa ttaattttta atcacctaaa aaaaatcctt
     3001 ctgaatatct tttcagaagg atttaaataa catattactt agcatcggaa tcacgaattt
     3061 cttttctgcg aatctttcca ctgattgttt taggcaattc atccacaaat tcgataatcc
     3121 ggggatattt gtaaggagca gtcaccgatt tcacatgatc ctgaatttct ttaatcagtt
     3181 catcaccagc cttgtctctg tattctttcg atagtattat ggttgctttc accacctgac
     3241 cgcggatttc atccggtact ccggtaatag cacattctac cacagcggga tgcgtcatca
     3301 gggcactttc cacttcgaac ggaccgatgc ggtatccgga acttttaatt acatcgtcag
     3361 cccggcctac aaaccagaaa taaccatcct catcgcgcca ggctacatcg ccggtgtagt
     3421 aaatattatc ctgataacat tcgtcggtta gctgctgatt gcggtgatat cctttgaata
     3481 agcctaccgg ataacctttg tcggttctga ttacgatctg accttgctcg cccacttcgg
     3541 ccgacctccc atcgggagta agtaaatcaa cattgtagtg aggacttggc agtcccatcg
     3601 agccgggttt tggctccacc cagggggctg taagtaccga tactgtcgtt tcgctctgac
     3661 catatgcttc gcggagtttt attcctgtca gtttgtagaa ctgtttgtac acttctggat
     3721 taagggcttc tccggcaatg gtacaatatt tcaatgaaga tgtatcgtat tttgtaaggt
     3781 cttcgcgaat taggaagcga aatatggttg gaggtgcaca gagtgaagta acccggtgtt
     3841 tttcaatcat tttcagcata tcagcaggtg caaatttatc gtgatcatag acaaacacac
     3901 atgcaccaac aatccattgc ccgtagagtt ttccccacac tgctttcagc cagccggtat
     3961 cggctatagt gaggtgaagg ctgttctcgt caaggttatg ccaatatttg gctgtaatta
     4021 tatgagccaa cggatacaca aaatcgagag ttaccatttt cggatttccc gtagttccgg
     4081 aagtaaaact gataatcatg ggatcgtcat tgctgttcac atggcttatg ggcgtaaatt
     4141 ccggagcatt tttaatgcct tcgtgaaaat cttgccagcc ttccgggatg attggtccga
     4201 ctgaaatcaa ttgtttgaca gacggagatt ctggtaatgc ttcactgata gccgatgcaa
     4261 ttggctcctc tcccactgca atgatggcag tgatatcggc agcattattg cggtaaacga
     4321 tatctttttt tgtaagcaga tgagtagccg gaatgatagt tatgcccagc ttatgcagcg
     4381 ctatgatggt gaaccagaac tcataacggc gtttgagaat agccattacg atgtctccct
     4441 tcttcaaacc cagggtctga aaataggaag ctgtctgatc ggtgtatttt ttcatctcgg
     4501 caaatgtgaa ttcgtggcaa tcacctttat cgttggtcca caggagtgct tttttatcgg
     4561 gttgcagggc tgcccattcg tctacaatat cgtatccaaa attaaattgt tcaggaacaa
     4621 tgagcttata attggctttg aaatcgtcaa agtcagaaaa ttctgtttgt tgtaaatatt
     4681 tttcaatcat aattattgta ctgtttgaga tttgacggtt cagactatca tcgccagaaa
     4741 aaccactttt tcgttgttga gtgccttcat tccgtgttgt ttagatgcgt cgaaataaat
     4801 gctgtcacct tcctccagta ccagatcgtt acccgcaata tggagctgca tagtaccctg
     4861 taacacaaag ttgaactcct gaccggtgtg agtattcagg gtcattggtt catcgttggg
     4921 ctccactgta acttcaaaaa gttctgcacg gttaccaaaa aagccagcac ccagcgcctg
     4981 atatttgtat gctttggtac gctctacact gatgcctttc cctttgcgtg taacgtaaaa
     5041 cgaactggca tgagctgttt cgccggagag gagttcggtt gtatcaagcc caaatgtctg
     5101 tgctacatgg aaaataaaac tcatgggaat atcagcatcg ccactttccc ataattcata
     5161 atcctgtttc gaaatttcgc atttcagggc aaattcttca gttgtgagct cgagagcctc
     5221 acgtaaaccc cgaagccttt cggcaatttg ttttatctga taattcataa actggtaact
     5281 tttttatata agttgatata ttactccagg taaaaataca cacaaaaaac caaggctaaa
     5341 acctgccagt gtttgtgaaa gagtatgcgc tttgagataa accctcgaaa cggcaacaaa
     5401 gccagtgata atcactgaaa cgactattaa accaacagga ttaacggcca tcaggtatga
     5461 ataggcgaaa actccaccaa ggaaaccacc cattccggca agatgtgcgc ttattttcca
     5521 tttcaggttg ataaacacca tcagaaatac agatagtacc gagccggctg ccacgagcga
     5581 aaattcaggc ggaaactgta aggttctcca taggaaaaac agccagaaaa catatgaaag
     5641 catcgaaaaa aggtaaggca tggtacgctc ctcccgtttg gaaataaata aatctttaat
     5701 ctgaccccgg cgcatcatca tgaaaatggg taaagccggt aataatccag taaacagtaa
     5761 tgttccagcc actgcaatga ttttgtatgc aggtgaaaat atactgaata aattcgtttg
     5821 cataagtaga atcatcccat aaaaaggcat caggagtggt tggaaaatgt aggaaataat
     5881 tttcaggaat gttctcatat ttttttactg tttgagattt atatttctat tctaagtctg
     5941 gccacgggaa tgttgagcag ttcgcggtat tttgccactg ttcgacgtgc gataatatac
     6001 cctttttcct tcagcagttc tacgagtttt tcatcggtta atgggtcacg cttatcttct
     6061 tcgtcgatac attccagcat aatctttttt atctctcttg tagatatctc ttcgcccgaa
     6121 tcattaatca tcgtttctga aaaaaagaac tttactggga aaattccaaa ttcagtttgc
     6181 acgtacttgc tgttgctcac acgagaaata gtcgaaatat catatccggt catatcggct
     6241 atctctttta ataccattgg tttaaggtat gtttcatcac cttcgataaa aaactctcgc
     6301 tgaaagttga cgatggcagt catcgtagta atgagagttt gctgcctttg tttaatcgca
     6361 tcaataaacc atcgggcagc atcgaccttt tgttttgcaa acataacggc atctttcatt
     6421 tccttattct gattctcttt attggatgaa tagtcgcgaa acatatcgtg atacgtactg
     6481 tttactctca gatcggggat attgctgttg ttgagctgaa cagtgagggt tccttcgtta
     6541 ttttcaacaa gaaaatcggg gactatagtt gacagtgatt tttcaagtac gttaccaccc
     6601 caggcgtttc cgggcttggg attcagacgg ataatctgag tcagcgcatc cttcagttcc
     6661 tcttcggtaa tattcatcga acggataatc ttttcataat gttttcggga gaattcatca
     6721 aagtaaccgg taataatcct tttagcgttc attacatcct gattgggctc ccttctttcg
     6781 agttgaagta aaaggcactc atgcaggtct tcggctccta ctccggccgg atcaaactgc
     6841 tgtacttcac gaatcaaccg cttcatttcg tcgtcgttgg tctgtatgcc tgcctgaaat
     6901 acgatatcgt ctaccataga ttcgggcgag cgtcgaagat atccctcatc atcaatattg
     6961 cctatgatgt attccatcag gttgcgttca tgactggtga gctgaagcaa tccaacttgt
     7021 tcggataaaa attcatggaa tgtcagtccc gcagaaaatg ggatctcttc atgcttgttg
     7081 tcaccagagc tattattgac catcagttta tagtccggaa tatcatcatc aggcatatat
     7141 tcttcaaaat ctacattgtc gtcagcctgg tattcatcgt ttgagtcttc ctcttcgtcg
     7201 gaattttcag ttttttccac accttcttcg agggcaggat tttcttccag ttcctgacgg
     7261 atacgctctt ccaactccac agtcggaatt tcgagcatct tgatgacctg aatctgggca
     7321 ggtgaaagtc tctgctgaag cttttgctga agttgctgtt ttaacataat tcgattcttc
     7381 tgtctgaaat agtcaaatga tttctcatgt gaggacaaag gtacattttt tcgttgaaaa
     7441 atttattaac aaaagtcatt ttaaagcttt acaagacttc tttgcaacag aaacttatac
     7501 ttgtattttt acaaactata atttactttg ttcgtttaat gatatctgct ttgagaaatt
     7561 gaattaattg agtagttttg cattggtatt ttcacctcaa tttttacctg ctgcatgtat
     7621 agtaaagatg aattaaaact gttgaaaaag gacttctggg aaggttttgg catatattgc
     7681 agcaatcatc cggtgcttgg aaaacggaag agtaaattca tgctgtacaa taccaaaatg
     7741 aaaggtgtgg aactcaagtt tgatgccaat cgcgaaggcg ctttcgttat tctggaactg
     7801 aacaacccga aggaagaaga ccgtctgaag agatacgagc agtttgagaa atacaaggct
     7861 gtgatggaag aaaattttcc cgaaggatta atctgggact ttgcttacaa gcttgaaaca
     7921 gggcatgaag tttgccggat ttacagccaa aaaaccggta ttgatatcca taaaaggatt
     7981 cattggatgg agttttacac atttatggct tccgaaatgc tgaaactcga aaaagctttc
     8041 agatacgtaa aagaagctgt agaatgagtg atcagataaa aagaaatgaa ccgaagtact
     8101 gaaattaagt ctgtttttca ggcaggacga agcaagtaat gatagaccag ctaaccattg
     8161 accgtatcaa cgaagccgct cacatcgaag aggtggtttc ggattatgtc actctgcgtc
     8221 gccgcggcgt gaacatgatt ggtttgtgtc cgttccacaa cgagaaaacg ccttcgttta
     8281 ccgtttcgcc cgccaagggt attttcaaat gtttcggctg tggcgaaggt ggcaattcgg
     8341 tacatttcat catgaaacag gagcaactct cttactacga ggcgctcaaa catcttgccc
     8401 ggaaatacca catcgaagtt cgcgaacggg aactttcggc tgaagaactg gcagcccgca
     8461 acgaccgcga aagcatgctg ctggtcaatg attttgctca aaaatacttt gccaacattc
     8521 tacaccatca cgtcgacggt aaatccatcg ggctcggcta tttccgtgaa cgcggcttcc
     8581 ggaacgatat catcgagaaa tttcagctcg gctattgtct cgacgaaaag gacgccttct
     8641 caaaatcggc catcaaagca ggttacaaca aagattttct ggtaaaaacc gggctgacca
     8701 tcgaaggaga taataactac ctggctgacc gtttccgcgg acgggtgatg tttccagttc
     8761 actccctgtc gggcaaagtg ctcgctttcg gaggacgggt gctgaaaaaa gacgagaaaa
     8821 cagccaaata tgtcaattcg cccgaaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnca ccccgatatt gagataagta aattcaccgc cgacctgatt ctggaaaaat
     9001 ataccctgag taaaatccat tcaaaatttc ataagcttga aacggaagtg gacagactct
     9061 cagaacttgc cccgcggttt attttcgagt acaaactggc aattatcatg gataaacgca
     9121 agcagaaact aatggaaatg aaaacagcag ccgaagcccg caacgataag atgatgcaac
     9181 aaatcatgag agaaattgaa gcaattgaca atttcaaaaa taaactgtcg aaagaactcg
     9241 acagggtgct aaacctgcta taaggttact tttccgttat cactcattcg aagataatat
     9301 caatactcag atcacaaaat atatgaaaac aattgaaaac acgtggcaaa aaaccgttgt
     9361 gaaagcctca cgttcattga atttgatttc agaagaaaaa attaacgaaa ccttgctggc
     9421 acttgcagat gccgctgttg ctgccactgg tgtaattttg acagctaacc agctcgatct
     9481 ggctgtcatg gagacttcca atccaatgta tgaccgcctg atgctttcgg aagagagaat
     9541 cagagctatt gcggatgata tccggaacgt agctgccctg ccctcgcccc tcggcaaaac
     9601 tcttatggaa accacaagac ccaacggaat gaatatccgg aaaatatcgg taccgttcgg
     9661 agtaatcgga atcatttacg aagcacgccc caatgtcagt ttcgacgtgt tttcactttg
     9721 tttcaagtcc ggtaatgcct gtgtgcttaa aggtggttcg gatgcctaca attccaatct
     9781 tgcgataaca catctcatca aaaatatatt gcgtcagcat ggacttgacg aaaatacggt
     9841 tactctgctc cctgccgggc acgaagctgc tgccgaaatg atgaatgcca cgggtatcgt
     9901 cgatgtaatt atcccccgcg gtggtaaagg tctgttagat tttgtacgta aaaattcaca
     9961 ggttccggtc atcgaaaccg gggccggaat tgttcatacg tacttcgacg aagatggtaa
    10021 cctagaaaaa ggcaaagaaa tcatattcaa ctccaaaaca cgccgtgtaa gcgtgtgtaa
    10081 tacactcgac tgcctgatta ttcatgagaa aagactgcca gacctgcctg ctctctgcga
    10141 aaaattagct gaaaaaaatg tcatcatcta tgccgatagc gcagcttatg gtgagctaaa
    10201 aggagtttat cccgtaacat tgcttcaaaa agcctccgaa gagagctatg gtatcgagtt
    10261 tctggattat aagatggctg taaaaacagt tgcagatatc cgggaggcgg tcgaacatat
    10321 cagcactcac agctcccgcc acagcgaatg tatcattacg gaaaacagga caacagcaga
    10381 acacttcaca aaagtggtgg atgctgcctg tgtatatact aatgtttcta cagcatttac
    10441 cgatggtgcg caatttggtt tgggtgcaga aatcggcata agtactcaga agcttcatgc
    10501 ccggggtccg atgggacttg aggaactttg ttcctacaaa tggattgtga gtggcagcgg
    10561 acaaacaaga agttaacaca attttttttg taacttgaaa gactgtgtca cttttcattt
    10621 aacaaactat aatcttttag tatcagaaca gtacagcaca gaacataatt tactatattt
    10681 ctattttttt tatgatttta gttgtttttt caaaaaaaat gtttttcttt gccttttgaa
    10741 agaaaagtgt atttttcgta cggtttaaat ccaacagttt atctaaatct ttttgaatga
    10801 ctattgcttt ttttaccctc cgtttttata ctatttctgt aaacacattt ttaattcatt
    10861 ttaattttat catgaacatt tacattggaa atctgagcta caaagttcgg gaaagtgact
    10921 tgcaaggtgt tataggagaa tacggtgaag tatcttcttg taaaattatc aaagaccgtg
    10981 aaaccggaaa atcgaaagga tttggttttg tggaaatgac cgacgatgct gccgcagcaa
    11041 aagctatcga agaacttaac ggagctgaac tcgacggacg tacacttgta gtaaaagaag
    11101 caagaccccg cgcttaatct ttttacacac taagaattaa agacttccat tcct
//
LOCUS       DAOPYB010000100        11021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6816_length_11021_cov_7.932975, whole genome shotgun sequence.
ACCESSION   DAOPYB010000100 DAOPYB010000000
VERSION     DAOPYB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6816_length_11021_cov_7.932975"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..470
                     /locus_tag="PKH58_08425"
     CDS             69..470
                     /locus_tag="PKH58_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNX89092.1"
                     /translation="MFSKNMEITGKIIAVLPETSGQGKNGTWRSRDYVLETSTNGEQY
                     PRKVCFNLFNDKIDKYPLAIDEVVTVSFDIDSREYNGRWYTSVKAWKVEKNAVNAAPA
                     ASQAASTNNASTNFTSFESANGADNGSDLPF"
     gene            633..1397
                     /locus_tag="PKH58_08430"
     CDS             633..1397
                     /locus_tag="PKH58_08430"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HNX89093.1"
                     /translation="MLFKTFIKLYKKTLFFAAGILLVTLLYLLISDHRKHHHEEEMKN
                     RIISDCDRSRYATAPISPTRELKDQNDVQLLHAQANGLKNVYISNASFEADSAQLVKD
                     QILIHLHNNPFYRVKELTHSYPYTTPEMGDLLNDINALFQEKMKDNKQDKYLFLVTSG
                     LRTNETQQELTNRNRNASTQSAHLYGATVDVSYKEFYNVDKDTVEPNFYAAEALRQTM
                     EDLREQCRMMIVRERKQACYHFTVVVCAPAQTVDTE"
     gene            1420..2325
                     /locus_tag="PKH58_08435"
     CDS             1420..2325
                     /locus_tag="PKH58_08435"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX89094.1"
                     /translation="MRRKSTYTPPPKMTRLKVSEQSLLMDFLLAKMGGMSRSSVKSLL
                     AHRQVTVNDKVVTQFDYTLKKNDSVIINSTRGNVELVHPKLRVIFEDSDLIVVEKKEG
                     LLSVTTGRETETTAFLILKNYVKKSSPRNKIYTVHRLDRETSGILVFAKNSDAQHILR
                     DNWHEIVTRRIYVAVVEGKVTKEKDRIVSWLTENEVSLKISSSKTDNGGKEAITNYRV
                     IKSNEEYSLLEIELETGRKNQIRVHMESIGHPIAGDRKYGSTSGFGRLALHARILEFY
                     HPMTSEVMHFETPVPKEFLKVFRNQ"
     gene            complement(2439..2975)
                     /gene="rsmD"
                     /locus_tag="PKH58_08440"
     CDS             complement(2439..2975)
                     /gene="rsmD"
                     /locus_tag="PKH58_08440"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNX89095.1"
                     /translation="MRIVSGKFKGRRIETPHTIQARPTTDFAKEGLFNVLNNWIDFEG
                     MNVLDLFAGTGSISLEFVSRGAKSVISVEQNERHCSFIRKTCSQLGVDNLLLTRGDVF
                     RLIATTRMKFDVIFADPPYELEQLPQLPDLIFKYDLLNAEGLFILEHSAKNNFENHPY
                     FSEHRTYGNVNFSFFEKK"
     gene            complement(2977..3696)
                     /locus_tag="PKH58_08445"
     CDS             complement(2977..3696)
                     /locus_tag="PKH58_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNX89096.1"
                     /translation="MSDKSHLYSADNTLDMRICPDGISYTVSDDNQQILLRQELNIPI
                     GPVTDMGIFEHFFSQPELQILGENVSIFYESNQYQLIPGELFSENDLEKIFTIEHSVS
                     ENEMILYKLLPKWGASLAFRIPARIQAFFEQKYPEAVIEHHISLLLKKQLANRETACW
                     LNIRKEMIDLIVIQNKQLQLLNSFQAKSNEDCCYYVLNVYEQLQLDTETFHLKIMSEK
                     SVNEQLFGLMKQYVRNVEIIK"
     gene            3928..6105
                     /locus_tag="PKH58_08450"
     CDS             3928..6105
                     /locus_tag="PKH58_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNX89097.1"
                     /translation="MNAENFKLYRKLLHYHSSLMSMEERRELRFLLKNFFSQHATANL
                     SADDYLFTKLQILDIILNEIGLGKPAIISVLFIEAVDYKIITAQELDKKFNTTVTPIL
                     HGLQRVKELYQKNVALESENFRKLLLTLAEDVRVILIIVAIRLQTMRNLDKMPEDERM
                     QIARESSFLYAPLAHRMGLYKVKTELEDLSLKYTNRDIYKEIAEKLNETKRSRDKYIA
                     EFIEPLKLKMAEIGFPFEIKGRTKSIHSIYSKIKKQNTAFEKIYDLFAIRIILDTPID
                     KEKAACWQAYSIITDMYQPNPNRLRDWLSIPKTNGYESLHTTVIGPQSKWVEVQIRTV
                     RMDEVAEKGLAAHWKYKGIKSESGMDEWLKNIREILENPELNAVDFMDEFKLNLYDKE
                     VFVFTPNGDLHKLPKGATALDFAFSIHTNLGSKCVGAKVNGKNVQIKYVLNNGDQVEV
                     LTSAAQKPNQSWLKIATTSKAKSKIRQALKEVEFKDAEIGRETLLRRFKNWKIEVDDG
                     RFSRLSKKKGFKTVSDFYQAIAQETIDIHEIRDAYLDMETRDSNPDIVIRTAEAFTKE
                     AAPIPDTASKEDVLVIGENLKNIDFKLAKCCNPIYGDNVFGFVSVTGGIKIHRTDCPN
                     APQMISRFGYRIVKAKWSGKSIGSQYPITLRVVGHDDIGIVTNITSIISKEKNVSLRS
                     ISIDTNAGLFQGNLTIMVDDTKELDSIIKKIKQVKGVKNVSRS"
     gene            6439..8898
                     /locus_tag="PKH58_08455"
     CDS             6439..8898
                     /locus_tag="PKH58_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX89098.1"
                     /translation="MKTKISIILFFFCSLMSISAQTHKVRIYGYVIDSNNRGIENVSV
                     YFEGKNNGTTTNHNGYYDLSIAMTDSVTLVYSHLGYQTIRHTILPDQRVVQITVEMPV
                     QVKELAEVNVMSIRRQTSTMETLDPSKYKLMPNSSGSFESMLIAFAGVSSTNELSSQY
                     NVRGGNFDENIVYVNGIEVYRPLLIRAGQQEGLSFINSDMVGHVGFSSGGFNSEYGDK
                     MSSVLDVRYKKPEGFEASASVSLLGASAYLGTAGKRFTQMHGIRYKTSQYLLGTLDTK
                     GEYNPSFVDYQTYMTWKLAPKWDINFLGNFSQNNFQFTPEERETAFGTYNKAEKLRIY
                     FDGKEKDLFQTAFGALTLNYKPLKDLKLGLTASSFHTNENETYDITGQYVLSELNLDT
                     DTAGQQGAQLGIGTYHEHARNRLKATVASLTHTGEFIRSGHTLKWGAALQQEIISDKI
                     SEWEWRDSAGYSMPYRYDKVVDLYYNLKAANTMSSWRTTTYLQDTYKWNNDAALWAIT
                     GGLRANYWTFNREFLLSPRASISVLPHWEKDFSFRFSTGVYYQSPFYKELRDTVSDSQ
                     GNVVIRLNKDIKAQRSLHFVLGGDYYFRALGRPFKFTTEAYLKLADRIITYSVDNVRI
                     RYSGENDAKGYTAGVDFKLFGELVPGTDSWINFSLMQSREDVLNDFYTDRTTNKTVYP
                     GWISRPSEQRYNFAMLFSDYLPNNPKYKLYLKTIFSDGLPVGPPRSQRYQAVFRTPSY
                     KRVDIGASRVIVNGSDRIMDRAWMKHIKNIWLNAEVFNLFDFRNVNSYYWVSDINKYQ
                     HGVPNFLTGRQLNIKILIDFM"
     gene            8993..10195
                     /locus_tag="PKH58_08460"
     CDS             8993..10195
                     /locus_tag="PKH58_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX89099.1"
                     /translation="MPLTSKRGKAMPESPIRKLVPLADKAKARGIKVYHLNIGQPDLQ
                     TPQIALEAIRKIDREILEYSPSDGFKSLRIKLASYYRTFNIDVNLDDIIITTGGSEAV
                     NFAFMSCLDPGDEIIVPEPAYANYTAFAIGAGAIVKPVVSTIEEGFALPHIEKFEELI
                     TPRTKGILICNPNNPTGYLYTRSEMNQIRDLVKKYDLYLFSDEVYREFCYTGAPYISA
                     FHLDGIEEHVILIDSVSKRYSECGIRIGALVTKNKEVRNNVMKFCQARLSPPLIGQIA
                     AEASMDTPADFMLDIYNEYVERRKFLIDGLNRIPGVYSPIPMGAFYTVARLPIDDSDK
                     FCAWLLSDFEYEGCTVMMAPASGFYTASGLGKDEVRIAYVLNKEDLKHALIVLAKALE
                     VYPGRTNN"
     gene            10415..10762
                     /locus_tag="PKH58_08465"
     CDS             10415..10762
                     /locus_tag="PKH58_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89100.1"
                     /translation="MKKTGLALLVLTTFYNLSFASPLLTRSKYLISVQSEAANGAVRT
                     GPVSYPLIYDSELSNETATDADYWIIKKQTDGTYTFQNYASKKYIKLLKYLLPVNIVL
                     FTISKSYNSGQLG"
     gene            complement(10806..>11021)
                     /locus_tag="PKH58_08470"
     CDS             complement(10806..>11021)
                     /locus_tag="PKH58_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89101.1"
                     /translation="GNQNKKQKPGQKANTNKPQTKQSRNAGNKSTVAATPKPEEPKSK
                     VVFVPKNKVNARQKTESSSNKYWGKNR"
BASE COUNT         3394 a         2358 c         2275 g         2994 t
ORIGIN      
        1 ttttcaaatt ataaattttt acattccccc gaacttaact atctttgcag tcagaaaatc
       61 aactaaaaat gttcagtaag aatatggaaa ttacaggaaa aataatagca gtattgcctg
      121 agacaagcgg tcagggtaag aacgggacat ggcgttctcg ggattatgtg ttggaaacaa
      181 gtactaacgg agagcaatac ccgcggaaag tgtgctttaa tcttttcaat gacaaaatag
      241 ataaatatcc acttgctatc gacgaagtgg taaccgtaag tttcgacatc gatagccgtg
      301 aatataacgg acgctggtac acctcggtaa aagcatggaa agtagaaaaa aatgccgtta
      361 acgctgcacc ggctgcttca caagcggcaa gtactaacaa cgcatccacc aattttactt
      421 cttttgaaag cgcaaacggt gctgataacg gttcggatct gcctttttaa gaattaatta
      481 atcaactaaa tgatagtgtg taaagcggga ataatcaccc gctttctttt ttatctgact
      541 gacacgaaaa acctaaaata tgcaaaaggg tttcgggtgc taattgccgg ttgaccgaaa
      601 agacgtactt ttgtgaaaac ttttttcaga acatgctatt caagactttc atcaaactat
      661 ataaaaaaac gctctttttc gctgccggta tccttctcgt cactttatta tacctgctta
      721 tatccgacca tcgcaaacat caccacgagg aagaaatgaa aaacaggata atatcggatt
      781 gcgaccgctc aaggtacgcc actgcaccta tcagccctac ccgtgaactg aaggaccaga
      841 acgacgtaca attgttacac gcgcaggcca acgggcttaa aaacgtgtat ataagcaatg
      901 cttcattcga agccgactcg gctcaacttg taaaagatca aatactgata catctgcata
      961 ataatccgtt ttaccgtgtt aaagaactga cccattctta cccctacacc accccggaaa
     1021 tgggtgattt gctgaatgat atcaatgctc tctttcagga aaagatgaag gataataaac
     1081 aggacaagta cttgtttctg gtaacctccg gactccgcac caacgaaacc cagcaggaac
     1141 tgaccaaccg taaccggaat gccagcacac aatcggcaca cctgtacgga gctactgttg
     1201 atgtttcgta caaagaattt tacaatgtcg ataaagatac ggtagaacca aatttttatg
     1261 ctgctgaagc cctgcgtcag accatggaag atctaagaga acaatgcagg atgatgatag
     1321 tgagagaaag aaaacaagcc tgttatcatt ttactgtcgt ggtttgtgca cccgcacaga
     1381 ccgtcgatac cgaataaacc tgtaaaaacc gataaaatta tgcgcagaaa atcaacttac
     1441 actcccccgc ctaaaatgac ccgtctgaaa gtgagcgaac aatcactgct gatggatttt
     1501 ttgctggcca aaatgggcgg catgagccgc agctcagtaa aatcgctgct ggcacatcgt
     1561 caggttaccg ttaacgacaa agtggttacc cagtttgatt atacactgaa aaaaaatgac
     1621 agtgtgatta ttaacagcac ccggggtaat gtagaactgg ttcatcccaa actgagagta
     1681 atcttcgagg acagcgatct gattgtcgtt gagaaaaagg aagggctgct ctctgtaact
     1741 accggcagag aaactgaaac tacggctttt ctgattttga aaaattacgt gaagaaaagt
     1801 tcaccccgta ataaaattta caccgttcac cggctcgacc gcgagacatc gggcattctt
     1861 gtatttgcca aaaacagcga tgcgcaacat atcctgcgtg ataactggca cgaaatagta
     1921 acccggcgaa tttatgtggc tgtagttgaa ggtaaagtga ctaaagaaaa agaccggatt
     1981 gtgagctggc tcaccgaaaa tgaagtttcg ctgaaaatca gctccagtaa aaccgacaac
     2041 ggcggtaaag aagccataac caactaccgc gtgataaaaa gcaacgaaga atattccctg
     2101 ctggaaattg aactcgaaac aggccggaag aaccagatac gcgtacacat ggaaagcatc
     2161 ggacacccca ttgccggtga tcgtaaatac ggttccacat ccggtttcgg acgactggcg
     2221 ctgcatgcac ggatactgga attctaccac cctatgacca gcgaagtgat gcatttcgaa
     2281 actccggttc cgaaagaatt tctgaaagtg ttcagaaatc agtaaaaaag ataaaactaa
     2341 tccgcattca tattatggtg gaattattat aagaaaaggt tcacacggtt atacataaac
     2401 ctgcgaacct ttttcatttg ttgtatatat agtcggtatt atttcttttc gaaaaaacta
     2461 aagtttacat taccgtaagt gcggtgttcc gaaaagtaag gatggttttc gaagttattc
     2521 ttagctgaat gctccagaat aaacagccct tcagcgttca gcaaatcata cttaaagatt
     2581 aaatccggta actgtggcaa ttgttcgagt tcgtagggcg gatcggcaaa aatcacatca
     2641 aacttcatgc gtgtggtggc aattaagcga aacacatcgc cccgcgtgag cagcaggtta
     2701 tcaacgccca actgactgca ggttttacga ataaaactgc aatgccgctc gttctgttcc
     2761 acactgatta cactctttgc tcctcttgaa acaaactcca ggctaatgct tccggttccg
     2821 gcaaacaaat caagcacatt cataccttca aaatcaatcc agttgttcag cacattaaat
     2881 aaaccctctt tggcaaaatc ggtcgttgga cgtgcctgga ttgtgtgagg ggtttcaatc
     2941 cttctgcctt tgaattttcc gcttactatc ctcatcttat tttatgattt caacgtttct
     3001 gacatactgc ttcatcaaac caaaaagttg ctcatttact gacttttctg acattatttt
     3061 taaatggaaa gtttcagtat cgagctggag ttgttcgtaa acattaagca cataataaca
     3121 acagtcctcg ttgcttttgg cctgaaagct gttgagaagt tgcagttgct tgttttgtat
     3181 aacaatcagg tcgatcatct ccttgcggat attcagccag catgcagttt ctcggtttgc
     3241 cagttgtttt ttaagcaaaa gtgaaatgtg atgttcaata acggcttcgg gatatttttg
     3301 ctcgaaaaat gcctgtatgc gcgccggaat gcggaatgcc agactggcac cccattttgg
     3361 aagtaacttg tacagtatca tctcattttc ggagacgctg tgttcgattg taaaaatctt
     3421 ctcaagatca ttttcagaga aaagttcgcc ggggatgagc tgatactgat tgctttcgta
     3481 gaagatgctg acattttcgc ccagaatctg caactcaggc tgactgaaaa aatgctcgaa
     3541 aatacccata tctgttacgg gaccaatagg gatattcaac tcctgacgga gtaaaatctg
     3601 ttgattgtca tcggatactg tataggaaat tccatccgga catatacgca tatccagggt
     3661 attatcggcc gaatacagat gggatttatc agacattaat taagaattta ttcctgttag
     3721 attgaaaaaa tatagttgca aaattaacaa agaaaaatca atccgagcta tacaataagg
     3781 atttctttta tttttcggcg tttttaatac ttttcaacag ctttagttag cgccctttca
     3841 attacgaatt tgtatatttg catcatgtac aacctgcgac atcagtcctg tttcggatgc
     3901 cgctttatca cactaataac ctcagtcatg aacgcagaaa actttaagtt ataccgcaaa
     3961 ctccttcact accactcatc gctgatgagt atggaagaaa gacgggagct acgatttttg
     4021 ttaaagaact ttttcagcca gcatgcaact gccaacctgt ccgcagacga ttacctgttc
     4081 accaaactgc agatactgga tattattctc aatgaaatcg gattgggcaa accggcaatt
     4141 atcagtgtat tgtttataga agccgttgac tacaaaataa ttacagcaca ggaactcgac
     4201 aagaaattca acaccactgt aacgccaatt ctgcatggat tacagcgcgt caaggaactt
     4261 tatcagaaga atgtggctct tgaatcggag aatttccgca aactgctgct gactctggcc
     4321 gaagacgtca gagtgatact tatcattgta gcaatccggc tgcaaaccat gcgtaatctg
     4381 gacaaaatgc ccgaagacga acgtatgcag atagcccgcg aaagttcctt cttgtatgct
     4441 ccgctggctc atcgtatggg tctgtataaa gtaaagaccg aactggaaga tctttcgctt
     4501 aaatacacaa accgtgatat ttataaggaa atagccgaga aacttaatga aaccaaacgt
     4561 tcgcgcgata aatacatcgc ggagtttatc gaacctctga aacttaaaat ggctgaaatc
     4621 ggatttccgt ttgagatcaa gggacggaca aaatctattc attccattta ttcgaagata
     4681 aaaaaacaaa atacagcatt cgagaaaata tatgaccttt ttgctatccg tatcatcctg
     4741 gatacgccga tcgataaaga gaaagcagca tgctggcagg catattctat cattaccgac
     4801 atgtatcagc ccaaccccaa ccgattgcgc gactggcttt ccatcccaaa aaccaacggt
     4861 tacgaaagct tgcataccac tgtgataggg ccgcaaagca aatgggtgga agtacagatc
     4921 cgaaccgtca ggatggacga agtagccgaa aaaggacttg ctgcacactg gaagtacaag
     4981 ggtattaaaa gcgaaagcgg aatggacgaa tggctcaaaa atatccgtga aatacttgaa
     5041 aatccggagc ttaatgccgt tgatttcatg gatgagttta aactgaattt gtacgacaag
     5101 gaagtttttg tttttacgcc gaatggcgat ttgcataagt tgcccaaagg tgctactgcc
     5161 ctcgattttg cattctcaat acataccaac ctgggctcca aatgcgtggg agcaaaagta
     5221 aacggaaaaa atgtacaaat taagtacgtt ctgaataatg gtgaccaggt agaagtactc
     5281 acttcagctg ctcagaagcc caatcagtcg tggctgaaaa ttgccaccac atccaaagca
     5341 aaaagcaaaa tccgtcaggc cctcaaagaa gttgaattca aggatgcaga aatcgggcgg
     5401 gaaacactgc tcagacggtt taagaactgg aaaatagagg ttgacgacgg gcgtttctcc
     5461 cggctttcga aaaagaaagg atttaaaacg gtaagcgact tttatcaggc tatagcacag
     5521 gaaaccatag atatacacga aataagggat gcatatctgg atatggaaac ccgggactca
     5581 aatcctgata tagtaatccg taccgccgaa gcgttcacca aagaagctgc cccaataccc
     5641 gacaccgcgt cgaaagagga tgtgcttgtg attggtgaga acctgaaaaa tattgatttc
     5701 aaacttgcaa aatgctgcaa tcccatttac ggagacaatg tctttggctt cgtatcggtt
     5761 accggcggta tcaaaatcca tcgcaccgac tgcccgaatg caccgcaaat gatttcgcga
     5821 tttggttacc ggatagtcaa ggcaaagtgg tcgggcaagt ctatcggatc acaatatcct
     5881 ataactctac gggtagttgg acatgatgat ataggtattg taaccaatat aacatccata
     5941 atctcgaaag agaaaaatgt atcgctccgt tccatttcca tcgatacgaa tgccggattg
     6001 tttcagggta atcttaccat tatggtcgat gacaccaaag aactcgattc tatcatcaaa
     6061 aaaatcaagc aggttaaggg tgtaaaaaac gtttcgagga gctgattatg cgtttatcag
     6121 ttgtcgatat acatattttt ataacataca tagtcggatt atctgatcaa actgatattc
     6181 tcagcagata gtccggctta ctaattgtaa ctatttttca ctacgcactt tcatttttca
     6241 tcagtaatga aattcaaaat ttctgatttg tattaatatt tcgacttcaa agtacaaatt
     6301 cacaacagtc gattttgcat aaacggcata ttatcattac ttttgcaact tatttaactc
     6361 aatacccata acacctacaa gcacaattag ccgtaaagta atgaatgatt ctgccatccc
     6421 gcactcacct gtaatctgat gaaaactaaa atatcgatca ttctgttctt tttttgttca
     6481 ctgatgagta tctccgctca gactcataaa gtgaggatat acggctatgt aattgacagc
     6541 aataaccgcg gaatagaaaa tgtcagtgta tatttcgaag gaaaaaataa cggcactacc
     6601 accaaccaca acggatacta tgacctcagc atagcgatga ccgattcggt tacgcttgtc
     6661 tattcacatc ttggctatca aaccattcgt catactatcc tgcccgatca gcgggtagtg
     6721 cagattacgg ttgaaatgcc ggtgcaggtc aaagaacttg ccgaggtgaa tgtgatgagc
     6781 atacggcgac agacctccac tatggaaact ttagatcctt cgaaatataa gctgatgcct
     6841 aacagcagtg gcagttttga gtcgatgctg atagcttttg caggggtaag ttcaaccaac
     6901 gaactgagtt cgcaatataa tgtcaggggt ggaaattttg atgaaaacat cgtatatgtg
     6961 aacggaattg aggtgtatcg tccgttgctg atacgtgctg gccagcagga aggtctgagt
     7021 tttatcaatt cagatatggt cggacatgta ggattttcat cgggaggttt caattccgaa
     7081 tacggcgata agatgtcatc tgttctggac gtgagatata agaaacctga aggttttgaa
     7141 gcttcggcat ctgttagtct gctgggagca tctgcctatc tgggtacagc aggaaagcgt
     7201 tttacgcaaa tgcatggtat ccgttacaaa acgtcacaat acctgctggg tacactggac
     7261 acaaaaggtg aatacaatcc ttcatttgtt gattatcaga catatatgac ctggaaactt
     7321 gctccaaaat gggacattaa ctttctgggc aacttctcac aaaacaattt tcagtttact
     7381 cccgaagaac gtgagacagc tttcgggacg tacaacaagg ccgaaaaact gcgaatttat
     7441 tttgacggca aggaaaaaga ccttttccag actgctttcg gagcgctgac tctcaattac
     7501 aaacccctga aagacctgaa actcggtctt accgcttcat catttcatac aaatgaaaat
     7561 gaaacttatg acatcaccgg acaatatgtg ctgagcgaac ttaatctgga cactgatact
     7621 gccggtcagc agggagcaca acttggtatc ggcacgtatc acgagcacgc ccgcaaccga
     7681 ttgaaagcta ccgtagccag cctgacacac accggagaat ttatacgctc cggtcacaca
     7741 ctgaaatggg gtgcagcatt gcaacaggaa ataatttctg acaaaataag cgaatgggaa
     7801 tggcgcgatt ccgccggtta ctccatgccc taccgttacg ataaagttgt tgatctgtat
     7861 tataacctga aagcggcaaa taccatgagc tcctggcgta cgacaaccta tctgcaggac
     7921 acttacaaat ggaacaacga tgcggctctc tgggcaatta ccggaggttt gcgggcgaat
     7981 tactggacct tcaaccgcga atttctgcta agtccccggg cttcgatttc tgtattgcca
     8041 cattgggaaa aagatttcag cttccgtttc agcacgggtg tctattatca gtcgcccttc
     8101 tataaggagt tgcgcgacac tgtttcagat agtcagggga atgtcgttat tcgcctaaat
     8161 aaggatatca aagcccagcg ttcgctgcac ttcgtactgg gtggcgatta ttatttccgc
     8221 gcactgggac gtccctttaa attcactacc gaagcttatc tgaaacttgc cgatcgcata
     8281 attacctatt ctgttgataa tgttcggata cgttattcgg gcgaaaatga cgctaagggt
     8341 tatactgccg gtgttgactt taagttgttc ggagaacttg tacccggaac agattcatgg
     8401 attaattttt cgctgatgca atcgcgggaa gatgtactga atgatttcta tacggataga
     8461 acaacaaata aaacggttta ccccggctgg atatcccgac cgagtgaaca gcgttacaac
     8521 tttgccatgc tgttttcgga ctatttgccg aacaatccca aatacaagct ctatttgaaa
     8581 accatttttt ccgatggttt acctgtggga cctccgagaa gccagcgtta tcaggctgtt
     8641 ttccgcacac catcgtataa acgtgtggac atcggagctt cacgtgtaat tgtcaacggt
     8701 tccgaccgga tcatggacag agcctggatg aaacacataa aaaatatatg gctgaatgcc
     8761 gaagttttca acctgttcga tttcagaaat gtgaactcgt attactgggt atccgatatc
     8821 aataaatatc agcacggtgt gccgaatttt cttaccggaa ggcagttgaa tataaaaatc
     8881 ctgattgatt tcatgtaact taatgatata tgaaagcaat ggaattcaga tgaacaaaaa
     8941 caggtcttga aagtatatta aataataatt cattaattca caacaaaata ttatgccact
     9001 aacatccaaa cgcggaaaag caatgccgga gtcgcccatc cgcaaactcg ttccactggc
     9061 cgataaagcc aaagcccgcg gcattaaagt ttatcatttg aatattggtc agcccgatct
     9121 tcaaacacct caaattgcac tcgaagcaat acgaaaaatc gaccgcgaga tattggaata
     9181 cagcccgagc gacggtttta aaagtctgcg tataaaactg gcatcctatt atcgtacttt
     9241 taatatcgat gtaaacctgg acgatatcat tattacaacc ggtggctctg aggcggtaaa
     9301 ttttgctttt atgagttgcc tcgatccggg tgacgaaatc attgtacctg agcctgccta
     9361 cgccaactac acggcctttg ccataggtgc aggagctatt gtaaaacctg tggtatcgac
     9421 cattgaagaa ggatttgcac tgccacacat agaaaaattc gaggaactga ttactcccag
     9481 aacgaaggga atacttattt gcaatcccaa taaccctacc ggataccttt atacacgaag
     9541 cgagatgaat caaatacgtg atctggtaaa aaaatatgac ttgtatcttt tctccgatga
     9601 agtatatcgt gaattctgtt atacgggcgc tccctatatt tccgcgtttc atctggacgg
     9661 tattgaagaa cacgttattc ttattgatag tgtatcaaaa cgttacagcg aatgcggcat
     9721 acgaataggc gctctggtta caaaaaataa agaagttcgc aataacgtga tgaaattctg
     9781 ccaggcaagg ttaagtccgc cattaattgg tcagatagct gctgaggctt ccatggatac
     9841 tccagccgac tttatgctcg atatatataa cgaatatgtg gaacgccgga aatttctgat
     9901 tgatggtctg aaccggatac ctggtgtata ctctcccatc cctatgggtg cattctatac
     9961 ggtagcccga ttgccaatag acgattctga taaattctgt gcgtggcttc tttccgactt
    10021 cgaatacgaa ggttgtacgg tgatgatggc ccccgcatct ggattttaca cggcttccgg
    10081 actgggaaaa gacgaagttc gcattgcata tgtacttaac aaggaagacc tgaaacatgc
    10141 actgattgtt cttgctaaag cactggaagt ttaccctgga agaactaata actaaccgga
    10201 taatttttca tacatacaaa gtatgcagac tgcgcgcctg catattttat tttatgcaag
    10261 atgaattacg gctctcagcc ggtgttatga ctgaattaaa cacaacaatt aacatcgaaa
    10321 gattaatgaa ataaattacg cttcaaatac tcagcatttt tgcattctta ttatacctat
    10381 gcggtgttaa tctcaaatga ttttatataa aaccatgaaa aaaaccggtt tagcccttct
    10441 tgtgctgaca actttttaca atttaagttt tgcaagtcct ctccttacaa gaagcaaata
    10501 ccttatttca gtacaatcgg aagccgctaa cggcgccgtt cgcaccggac ctgtcagcta
    10561 tccccttatt tatgattctg aactgagcaa cgaaactgct acagatgctg attactggat
    10621 aattaagaag caaaccgatg gtacctacac gtttcagaat tacgcgtcga agaaatacat
    10681 aaaattactg aaatatttac tacctgtaaa tattgttcta tttactatat ccaaaagtta
    10741 taactcagga cagttaggat gatataatca tgacattatg cgtatattcc caacaacaat
    10801 atcagctatc ggttttttcc ccaatattta tttgaactgc tttcggtttt ctgccgggca
    10861 tttactttgt tctttggtac aaacaccact ttgctttttg gctcttccgg tttgggagtg
    10921 gcagccacag tcgatttgtt tccagcgtta cgtgattgtt ttgtctgtgg tttattcgta
    10981 tttgctttct gaccgggttt ttgttttttg ttctggttac c
//
LOCUS       DAOPYB010000101        10870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6948_length_10870_cov_10.606750, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000101 DAOPYB010000000
VERSION     DAOPYB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6948_length_10870_cov_10.606750"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(512..1624)
                     /locus_tag="PKH58_08475"
     CDS             complement(512..1624)
                     /locus_tag="PKH58_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX89102.1"
                     /translation="MKTLMVVLRKEFLLIFRDKMILPMMFVMPVVQLILFPFAADLDV
                     RELKIVVVDNDHSSYSQQLVLKIASTDLFQIYAAPNSYKDALRYIGKNQADIILQIPQ
                     GFERGLTRENSSEVAIAADAVNIVKAGLGTSYLITIIQNFNQQIRLEWNQGVKMTAPV
                     ELEYSFWYNPFMKYRNYMVPAILVLLMTVLSGFISALNIVREKEEGTIEQINVTPLKK
                     WQFVLGKMIPFWLIGIFVMSLGIAIMHFVYGIHIEGSLGTLYLFGMVYITGILGLGLL
                     ISTFAHTQQQSMFISFFFIMILILLSGLFTNVESMPRWAFIFSDLLPITHFMHAVRAI
                     VIKGSAFLDIWKDMVYIIVFSIIINMLAVLNYRKTN"
     gene            complement(1621..2763)
                     /locus_tag="PKH58_08480"
     CDS             complement(1621..2763)
                     /locus_tag="PKH58_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX89103.1"
                     /translation="MRTKEKNISQKQRGIKVLRAFIHKEFLHVFRDKRTLLIMFVLPI
                     VQILIFGFALTNEVKNIRLLVVNPTNDVASQQLIEKIKASSYFTIKHVEPTPRNVDNY
                     FKNSEIHCALILPENMSSKSNETTEIQIITDASDPNFSKIAINYLSATITDFLNKPNV
                     VQKMPMQIKTETRMLYNPSMNGSMNFVPGLIALIMMIVCTTLTAVSIVREKEFGMMEV
                     LLVSPIRPIFVLISKAIPYFLLSSVNLIIILVLSNVVLNVPIRGSLFLIVFVSMLYIL
                     TCLALGLLVSNVTSSQAFAMMFSMAGILLPIILFTGFIFPIENMPIGFQWFSNILPSR
                     WFFLIMQSVMLKGRGFADVWLEILVLFTMMIVLTAISLKKFKIRLQ"
     gene            complement(3069..3809)
                     /locus_tag="PKH58_08485"
     CDS             complement(3069..3809)
                     /locus_tag="PKH58_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX89104.1"
                     /translation="MKFSIETNDLVKKFGDFTAVDHITFQVSEGEIFGFLGANGAGKT
                     TAMRMLCGLSYPTSGKATVAGFDVYKEQEKIKKSIGYMSQKFSLYENLTVKENIEFYG
                     GVYGLKRKEINEKLKIVLRELDMEEMKNKLVKSIPLGWKQKLAFSTAIFHEPKIVFLD
                     EPTGGVDPITRRQFWNMIYGAAERGITVFVTTHYMDEAEYCNRISLMVDGRIEAFDTP
                     ANLKKTHHAASMDEVFYSLARKAKRDDG"
     gene            complement(4102..4758)
                     /locus_tag="PKH58_08490"
     CDS             complement(4102..4758)
                     /locus_tag="PKH58_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89105.1"
                     /translation="MKKEVYRGNLPHFQQPGQDYFVTWILKNSVPKKAVEVYSKRMEQ
                     LKNEIDILKRNNADTNAIENAKKDYYIIRKKYIAKVDEILDAKTDNIVDLSKSENLNI
                     IYETLTFWEGKRIGNYCFCVMPNHVHWVLRVFEKDTKEKPVYLEEIMHSVKLFTSRQI
                     NIFENRKGTLWNKESFDTTIRDRIHLHRAIEYTLNNPIKANLVKNREDWKGNFNFERD
                     "
     gene            complement(4935..5834)
                     /locus_tag="PKH58_08495"
     CDS             complement(4935..5834)
                     /locus_tag="PKH58_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX89106.1"
                     /translation="MNYIELTNISKTYEKSSLPAVKNVNFDVEKGEIFGMIGADGAGK
                     TSIFRMLTTLLLPDSGSAKVLGYDVVKDYQEIRKEVGYMPGRFSLYQDLSVEENLNFY
                     ATLFGTTVEENIDLIRDIYVQIEPFKKRRAGKLSGGMKQKLALCCALIHKPRVLFLDE
                     PTTGIDPVSRKELWEMLRKLKQEDIAILVSTPYMDEALQCDRIGLMKNGEMMKIGTPQ
                     NMINSYYKPLYAVYSDNIFHLLQILEKYPNRINSYAFGDKVHVIVDKSEKELFDYLTE
                     KNFKDIQIEKIKPTVEDCFIDLS"
     gene            complement(5913..6845)
                     /locus_tag="PKH58_08500"
     CDS             complement(5913..6845)
                     /locus_tag="PKH58_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNX89107.1"
                     /translation="MKTKLSKCSLTLPLGGWGAGLLLFFILLFSCKSKDNNYDASGYF
                     EAVETIVSAQANGQILSLNVDEGQELAAGQQVGQIDSTQIYLTLQQVQSQRGAVLSRK
                     PDIGSQTAVLKEQITTAKRELARVERLAAGNAATQKQMDDARSAVAVLQKQLDAQQTS
                     LSNASSSVSKEGSPLEVQMSLLKDQLNKTHIINPVNGTVLVKYVEQNEMASMGKPLYK
                     IADLSEIILRAYITNTQLANLKLGQKVTVLVDKGKDKNAEYQGNIEWISSKAEFTPKT
                     IQTKDERSNEVYAIKIRVKNDGFLKIGMYGDVKF"
     assembly_gap    7153..7252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7253..8184)
                     /locus_tag="PKH58_08505"
     CDS             complement(<7253..8184)
                     /locus_tag="PKH58_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX89108.1"
                     /translation="MKKIILITLLLFSIISANSQTITLQECQDLAQQNFPLVRQQNLI
                     EQTAKNSSSAILSRYFPQVQVNAQATYQSDVTKIELGNLPAQMQSISFPTPDKDQYKA
                     FVDVSQVLYDGGAITAQRKLVDANSRVEKEKVAVELYKLKDRINQTYFGILLMDEQLK
                     QTDLLQNDLQTALDKVNVMVKNGVSLISNKQNLEAEMLKLKQNRQEIQTNRQSLIQVL
                     ALLTGKEMTENTSFAKPQSQLADEVAQRPELSLINSQLNTVKLLNNQLNTTILPKANL
                     FFQGGYGKPALNMFKNEFEPYYVAGVRLNWQLSG"
     gene            complement(8293..8838)
                     /locus_tag="PKH58_08510"
     CDS             complement(8293..8838)
                     /locus_tag="PKH58_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HNX89109.1"
                     /translation="MSHLKFLIAMLPVVFMLHDFEEIIMFKPWLIKNREELKSRFPKF
                     ENFLTKRSYFEYSTSAFAVAVMNEFALISLVTFCSLYFDVYDWWFAAFMAYSIHLVVH
                     LGQWIIYRKYVPVIITTVLTFPYCIYTFCVFKDSSMMRIEQMLLWTAIGVGLTILSFF
                     PAFYFASIFEKWKRTNYLMKK"
     gene            complement(8881..9507)
                     /locus_tag="PKH58_08515"
     CDS             complement(8881..9507)
                     /locus_tag="PKH58_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX89110.1"
                     /translation="MKHVEKKEMDKTTEEKIKLAAGKIFMEKGFAGARTRDIAQEAGI
                     NLALLNYYFRSKENLFEIVMLDKLKSFFDVIVNIVIDTGSTYEEKLPKLVEKYTDLLM
                     KEPNLPFFLLHELPQNPNFFDEKLHIKDKINLQSFAFFQSTLKLEKKEDALQLLITFL
                     GMMIFPFIVRPAIQSLFDISEDDYTKILLDRKTLIPQWINETVKKRIE"
     gene            complement(9514..10278)
                     /locus_tag="PKH58_08520"
     CDS             complement(9514..10278)
                     /locus_tag="PKH58_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX89111.1"
                     /translation="MKLENKRIVITGATSGIGLHLTKTLAEIKGAQIIAVGRKIENIP
                     NAPNIIPLRADVSKPEEVDKLFQFAVEKMGGIDVFFANAGFAYYEKLGASSWEHVESI
                     FSTNVVSPIYSLTKMIELNQSSEFMVVITCSVIGKLPFPGFSLYTGTKFALDGFNSAM
                     QYEMPANGHLMMVYPVATYTEFFNRAAEKSNMPWPHQHTSDVIKAVMKGIAANKKRVF
                     PFPLIRPVLYLGNIFPFFYKMYWNVQKKKLNQLSNS"
     gene            complement(10620..>10870)
                     /locus_tag="PKH58_08525"
     CDS             complement(10620..>10870)
                     /locus_tag="PKH58_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNX89112.1"
                     /translation="PDGKMYFNSTGNPGMASGGMGDVLTGIIVGLLAQGYSSTDSAVL
                     GVYLHGLSADLALQTQSEESLLASDVAGNLGNTFKHLR"
BASE COUNT         3253 a         2143 c         1718 g         3656 t
ORIGIN      
        1 gaattagtga gaataaaaat gcaggttacg gaaaactgta acctgcattt ttattctttg
       61 aagtaacttt ataaacagga taatttacga taaaaattat cagttgacaa cagacatcac
      121 ccccaatgtg tgtattcgtt taatgaatga ataagatgcg ccatcatact tttattgtcg
      181 ttttttagca gcaaaccata ccgggaaaat cgtaaaagaa tcactattgt ccgataaaat
      241 ttaatattta gccacttccg acaaatcgga atatgccatc tgtggcacaa aatcttatct
      301 tttcatcttg ctacaaagat ttagcaactc tgtagctgtt tttgcaacag cgttgcaaaa
      361 tatttgtagc gatttggtaa caacaggaaa aaaagccccg gcggggctta atgttcttat
      421 tagtttcaat tacagtaaat tacagatgtt tttgtattat ttatcagaca acaatataaa
      481 agaatatcgt tatttttcct ttttgatgtt tttaattcgt tttccgataa tttaatacag
      541 ccaacatatt tatgattatt gagaatacga taatatagac catatctttc catatatcca
      601 ggaaagcact tcctttgata acgatagctc taactgcatg cataaagtgt gtaatcggca
      661 ataaatccga gaaaatgaat gcccagcggg gcatactttc cacattggta aacagtccgc
      721 ttagaaggat aagaatcata atgaagaaaa atgaaataaa catagactgt tgctgcgtat
      781 gggcaaaggt cgaaatcagg agtcctaacc ccaaaatacc tgtaatatac accatcccaa
      841 aaagatataa agtgccaaga cttccctcaa tgtgaatgcc gtaaacaaaa tgcataattg
      901 ctattccgag cgacattaca aaaattccta ttaaccaaaa ggggatcatt tttcccagta
      961 caaactgcca tttttttagc ggagtcacat tgatttgttc gatagttcct tcttcctttt
     1021 cacgcacaat attaagtgct gaaataaatc cacttagcac cgtcattaac aatacaagaa
     1081 ttgccggtac catataattt ctatatttca taaatggatt gtaccaaaac gagtattcca
     1141 actctaccgg agcagtcatt ttcacacctt gattccattc caatcgtatt tgctgattga
     1201 aattttgaat aatagtaatg agataactcg ttcccagacc tgctttgaca atatttaccg
     1261 catctgctgc aatagctact tcagaactat tctcgcgcgt taaaccgcgt tcaaaaccct
     1321 gtggtatctg gagaataata tcagcttggt ttttaccgat atatctcaat gcatctttat
     1381 aggaattggg agctgcgtaa atctgaaaca aatcagttga agcaattttt agaacaagtt
     1441 gttgtgaata gctgctatga tcgttgtcaa ccactacaat tttcagttcc cgtacatcca
     1501 aatctgcggc aaaagggaat aaaatcaact gtaccacagg catcacaaac atcatcggca
     1561 aaatcatttt gtctctgaaa ataagcagaa attcctttcg caatacaacc atcaacgttt
     1621 tcattgtaat ctaattttaa atttcttcaa actgattgca gtcaatacaa tcatcattgt
     1681 aaacaatact aatatttcta accagacatc agcaaaacct ctacctttga gcattacaga
     1741 ttgcatgata aggaaaaacc agcgagatgg gagaatgttg gaaaaccact gaaaaccgat
     1801 aggcatattc tcaatgggga atataaaacc ggtaaaaaga ataatcggaa gcagtattcc
     1861 agccatcgaa aacatcatag caaatgcctg tgaagatgtt acattggaca ccaacagtcc
     1921 caatgctaaa catgtgagaa tataaagcat acttacaaat acgattaaaa ataagcttcc
     1981 cctaatcgga acattcaaca ccacattact taataccagt ataattatca gattgacaga
     2041 tgaaagtaaa aaatacggta tagctttgga aatcaatacg aaaataggac gaataggtga
     2101 aacaagcagt acttccatca tcccaaattc tttttcccga acaatggaaa cagctgtaag
     2161 tgtggtacag acaatcatca ttatcagagc aattaatccc ggcacaaaat tcatcgaacc
     2221 gttcattgaa ggattataaa gcattcgtgt ctcggttttt atctgcatcg gcattttctg
     2281 gacaacatta ggtttattca gaaagtctgt aatagtagca gaaagataat tgatagcaat
     2341 ttttgaaaaa ttcggatcgg atgcatccgt aataatctga atttctgtcg tttcattgct
     2401 tttggaagac atattctcag gcaatatcaa agcacagtgt atctcgctgt ttttgaaata
     2461 attatctacg tttcgcggag taggttcaac atgttttatc gtaaaatacg aactcgcttt
     2521 tatcttctct atcaattgtt gcgaagcaac atcattcgtt ggatttacta ccaaaagacg
     2581 tatatttttc acttcattcg ttaaagcaaa tccaaatata agtatttgta caataggcaa
     2641 tacaaacata atgagcaatg ttcgtttgtc tctgaaaacg tgcagaaatt ctttgtgaat
     2701 aaatgctcta agaactttta ttcccctttg cttttgtgat atattttttt ccttagttct
     2761 catttgttgt tacagttttc ttcaattcct tataaagcat tatctgattt gaattttttc
     2821 tcactatcat aatacactat tgtccggtaa aattctaaat tcttgaaaaa tattagattt
     2881 ctcactttgt tcgaaatgac agtcagcatt ttgtcttttg ggagggggtt tggtaggcgg
     2941 cgaagccgcc aaacaaaccc cctccctcca taaatctcca taaatccctg tcatccagag
     3001 cgcagcgaag taaccaaata tctttaccgg acaacatcaa ttataatatc atacaaagct
     3061 gtatctattt acccatcatc tctctttgct tttcttgcca acgaataaaa tacttcatcc
     3121 atcgaagctg catgatgcgt ttttttaaga ttggcaggtg tatcaaacgc ttcaatcctt
     3181 ccgtccacca tcagtgaaat acggttacaa tattccgctt catccatata atgagttgtt
     3241 acaaaaacag ttattcctct ttcggcagcg ccataaatca tattccagaa ttggcggcgt
     3301 gtaattgggt caactcctcc tgttggctca tccaaaaaca caattttcgg ttcatgaaaa
     3361 atcgctgttg agaaagcaag tttttgcttc cagcccagtg ggattgattt caccaattta
     3421 tttttcattt cttccatatc caattccctg agcacaattt tcagtttttc gttgatttct
     3481 ttccgtttaa gtccatacac accaccgtag aactcgatgt tttcttttac tgtcaggttt
     3541 tcatacaacg agaatttttg gctcatatat ccgatgcttt tttttatttt ctcctgttct
     3601 ttatacacat caaatcctgc cacggttgcc tttccactcg ttggatacga caaaccgcat
     3661 aacatacgca tggcagtagt ttttcctgct ccgttggcac ccaaaaagcc gaatatctct
     3721 ccttcgctta cctgaaatgt aatgtgatcc accgcagtaa aatcaccaaa tttcttaacg
     3781 agatcgtttg tttctattga aaatttcata gctgttttcg ttttactgtc aataaatcgt
     3841 tcgtcctaaa gcaatgtttc ctgacttggg ttaaacgatt tcctaatcgt ttatgtgttt
     3901 cttccaacaa aaaggatttc gttgttccca atcgcaggtt gtctgacttg ggttaaacga
     3961 tttccaaatc gtttatgtgt ttcttccaac gaaaaggatt tcgttgttcc caatcgtagg
     4021 tttcctgact tgggttaaac gatttccaaa tcgtttaata tctcctacga aaaggatttc
     4081 gttgctccca attgcaggtt ctcaatctct ttcaaaatta aaatttcctt tccaatcttc
     4141 tctatttttc accaaattag ctttaatcgg attatttaaa gtatattcta ttgcacgatg
     4201 tagatgaatt ctatctctta ttgttgtgtc aaaactttct ttattccaca gggttccctt
     4261 cctgttttca aaaatattta tttgtcgaga agtaaaaagt ttaactgaat gcataatttc
     4321 ttcgagataa acaggttttt ctttggtgtc tttttcaaaa acccgcaata cccaatgaac
     4381 gtgattaggc attacacaaa aacaataatt tcctattctt ttcccttccc aaaacgtgag
     4441 tgtttcataa ataatattca gattttcaga tttggacaaa tcaactatat tatccgtttt
     4501 tgcatctaaa atttcatcaa cttttgcaat atattttttc ctaataatat aataatcctt
     4561 ttttgcattc tcaatggcat ttgtatcagc attatttcgt ttcaagatgt caatttcatt
     4621 ttttagctgt tccattcgtt tgctgtaaac ttctactgct ttttttggaa cagaattttt
     4681 caatatccat gtaacaaaat aatcttgccc gggttgctga aaatggggta aatttccccg
     4741 ataaacttcc tttttcatag ctttctgttt atatctttta tatttccaac gaaaaggatt
     4801 tcgttgttcc caatcgcaaa ttttctgatt gggataaacg atttcctaat cgtttaatct
     4861 ttcaatgaat ctggattggg ataaatgatt tcctaatcgt ttatgttatt taatattaaa
     4921 cccgaattct ccaatcagga caagtcaata aaacaatcct caaccgttgg tttaattttt
     4981 tcaatctgaa tatctttgaa atttttttct gtaagataat caaaaagttc tttttcagat
     5041 ttatctacta ttacgtgaac tttatctcca aaagcataac tgtttattct gttcggatac
     5101 ttttccaaaa tttgtaatag atgaaatata ttgtcggaat aaactgcgta caacggttta
     5161 taatagctgt ttatcatatt ttgtggggtg ccaattttca tcatttcgcc gtttttcatc
     5221 aacccgatgc gatcacactg caacgcttca tccatgtaag gtgtcgaaac caggatagca
     5281 atatcttcct gcttgagttt tcggagcatt tcccataact ctttacgtga aacaggatcg
     5341 ataccggtag ttggttcatc cagaaaaagt actctcggct tatggatcaa cgcacaacat
     5401 aacgccaact tctgcttcat ccctccagaa agcttacctg cgcgacgttt tttgaacggt
     5461 tcaatctgca cataaatatc tcggattaaa tcaatatttt cctccacggt tgttccgaat
     5521 agtgttgcat aaaaattcag attttcttcc acactcaaat cctgatacag cgaaaaccgc
     5581 cccggcatat aacccacttc ttttcgtatt tcctgataat cttttaccac atcgtatcca
     5641 agtacctttg cagagccact atcaggtaaa agcagagtag tcagcatacg aaaaattgaa
     5701 gtcttccctg ccccatctgc tcctatcatt ccgaaaattt cacccttttc cacatcaaag
     5761 tttacgtttt taactgccgg cagagagctt ttttcgtatg tttttgaaat attagttaat
     5821 tcaatatagt tcattttctt gtattttcgg cgatttggat ttgaaaatcg acgtagtcaa
     5881 atcgctgttt ccaattataa catttcggct ttttagaatt ttacatctcc gtacatcccg
     5941 attttcaaaa atccatcatt tttcacacga attttaattg catacacttc attggagcgt
     6001 tcgtctttcg tttggatggt tttaggagta aattcagctt tggagcttat ccattcaata
     6061 ttcccctgat attcagcatt tttatctttt cctttatcca ccaaaaccgt tactttctgt
     6121 cctaatttca ggtttgctaa ttgtgtatta gtaatatatg cacgcaaaat aatttctgac
     6181 aaatcagcaa ttttgtacaa gggttttccc atcgaagcca tctcattttg ttcaacgtat
     6241 ttaactaaca cagtgccatt tacaggatta ataatatgtg ttttatttaa ttgatctttc
     6301 agtaatgaca tctgtacttc taacggacta ccttctttgc ttacgctgga agaagcattg
     6361 gacagcgaag tttgttgtgc gtccaactgt ttttggagta ctgcaacagc tgatcgagca
     6421 tcgtccatct gcttttgtgt agcagcattt cctgccgcaa gacgttccac acgtgctaat
     6481 tcacgtttgg ctgttgtgat ttgttctttc aaaacagctg tttgagagcc tatatccggt
     6541 ttgcggctta aaacagctcc acgttgcgac tgcacttgtt gtaaagtaag ataaatttgg
     6601 gtactatcta tctgtccaac ttgttgtcct gctgcgagtt cctgtccttc gtccacattc
     6661 agcgacaata tttgtccatt tgcttgagca gaaacaatgg tttcaactgc ttcaaaatat
     6721 cccgaagcat cgtaattatt gtcttttgat ttgcaggaaa agagaagaat aaaaaataaa
     6781 agcagacctg ccccccaacc ccctaaaggg agagttaatg agcattttga taattttgtt
     6841 ttcatgtgtt tataatttat tatttaattg tcacatggaa ctcgcacata aacatcacct
     6901 tgtatgtcaa aatttatttg tcgtgctcgt ttcatattat tttgttttat ttgttttatt
     6961 atttcaaact taatctattt cattttgtcc caaaatcctc cctttaggag gttagggggc
     7021 aatccacctc aaattatact ctgccaatag caattgtatt tcgtgcagtg atttggtttg
     7081 acgagcttgg ttttcagcat tggcatcccg aataaaatca gaacttgtca acactccatt
     7141 ttcgagttga acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaagcccga
     7261 caattgccag tttaatcgca ctccagcaac gtaataaggt tcaaactcgt ttttgaacat
     7321 attgagtgcg ggtttgccat aaccaccctg aaaaaacaga ttagcttttg gaaggatagt
     7381 cgtattcagt tggttattca gcaatttcac cgtatttaat tgcgagttaa tcagagatag
     7441 ttcgggacgt tgagccactt catcagctaa ttgcgattgg ggttttgcaa aggatgtatt
     7501 ttccgtcatt tctttaccgg taagcaaagc cagaacttga ataagcgact gacggtttgt
     7561 ctgaatttcc tgccgatttt gtttcagttt cagcatctca gcttccagat tttgcttgtt
     7621 gcttatcaat gaaactccgt tttttaccat cacattcact ttatccaatg ctgtttgtaa
     7681 atcattttga agtaaatcgg tttgcttcaa ttgttcgtcc atcagtaaaa taccgaaata
     7741 agtctgattt attctatctt tcagtttgta gagttcaacc gctacttttt ccttttccac
     7801 acgactattc gcatccacca attttcgttg ggcagtaata gcaccaccat cataaagcac
     7861 ttgcgacaca tctacaaaag ctttatactg gtctttgtca ggtgtaggga aacttatcga
     7921 ctgcatttgt gccggaagat taccgagttc tattttcgtt acatccgatt gataagtggc
     7981 ttgtgcgttt acctgaacct gtggaaaata gcggctcaga atggcagatg aactgttttt
     8041 agccgtttgt tctatcaaat tttgctgccg caccagagga aaattctgct gtgctaagtc
     8101 ctgacattcc tgcaatgtga ttgtctgcga atttgcagat ataatactga atagcagcaa
     8161 tgtaattaat attatttttt tcatacgatt tgaaaatttg tatttttatt ttttttgatg
     8221 ctcataattg taaacaacaa cgaaggtttt caaccattcg ttgagtttaa atttacttaa
     8281 aatccgtttt atttattttt tcatcaaata attcgttctt ttccatttct caaatattga
     8341 agcgaaataa aaagcaggga aaaaactcaa aattgtaagc cctaccccaa ttgctgtcca
     8401 caaaagcatt tgttcgatac gcatcatgga tgaatcttta aaaacacaga atgtataaat
     8461 gcaataagga aatgtcagaa cggttgtaat aatcacggga acatattttc ggtaaataat
     8521 ccactgcccc agatgaacaa ctaaatggat agaataagcc ataaacgcag caaaccacca
     8581 atcatacaca tcaaaataca aggaacaaaa agttacaaga gatattaaag caaactcatt
     8641 cataacggca acagcaaagg ctgatgtaga atattcgaaa taacttctct tagtcagaaa
     8701 attttcaaat ttcggaaacc gagatttcaa ttcttctcta tttttaatga gccagggttt
     8761 gaacataatt atttcttcaa aatcgtgaag cataaaaacc acaggcaaca ttgctattag
     8821 aaatttcaga tgtgacatag taattctatt ttgaacttta ttatttctat ccgtcagaat
     8881 ttattcaatt cgttttttta cagtttcatt tatccattgt ggtatcaatg tctttctatc
     8941 caataatatt tttgtgtaat catcttccga gatatcgaat aaactttgaa tagcaggtcg
     9001 cacaataaaa gggaaaatca tcattcctaa gaatgtaatt aacagttgta aggcatcttc
     9061 ctttttctca agctttaatg tactttgaaa gaatgcaaac gattgtaagt ttatcttatc
     9121 tttgatatgc aacttttcat cgaaaaaatt tggattttgc ggcaattcgt gcaacagaaa
     9181 gaaaggcaga ttgggttcct tcatgagaag atctgtatat ttctcaacca attttggcag
     9241 tttttcttca taagtagaac ctgtgtcaat aacaatattt acaatgacat caaaaaacga
     9301 tttcaactta tccagcatca caatctcaaa cagattttct ttactccgaa aataatagtt
     9361 cagaagtgcc aaattaattc ccgcttcctg agcaatatca cgcgtacggg cacctgcaaa
     9421 tcctttttcc ataaatatct ttccagcggc aagttttatc ttttcttcag tggttttatc
     9481 catctctttt ttctcaacgt gtttcatgtt tatttagcta ttagacaatt gattaagttt
     9541 tttcttctga acattccaat acattttata gaaaaacgga aagatattac ccagataaag
     9601 aaccggacgt atcaacggga aaggaaaaac acgttttttg ttagctgcaa ttcctttcat
     9661 cacagctttt atcacatcac ttgtatgttg atgaggccaa ggcatattgc ttttttcggc
     9721 agcacgattg aaaaactcgg tgtaggtagc caccggataa accatcatca gatgtccgtt
     9781 agctggcatt tcgtattgca tagctgaatt aaatccgtct aatgcaaact tagttcctgt
     9841 gtagagcgaa aaaccgggga atggaagttt accgattacc gaacaagtga taactaccat
     9901 aaattcagag gattggttaa gctctatcat ttttgtcaac gaataaatgg gcgaaaccac
     9961 gttggtagaa aaaatacttt caacgtgctc ccaactcgat gctcccagtt tttcgtaata
    10021 ggcaaaaccg gcatttgcaa agaaaacatc aattccgccc attttctcaa cggcaaactg
    10081 aaaaagttta tccacttctt ccggttttga aacatctgct cgtaaaggaa tgatgtttgg
    10141 tgcattcggt atattttcaa tttttctacc tactgcaata atttgagcac cttttatttc
    10201 ggctaatgtt tttgtcagat gcagtccgat gcccgatgtg gcacccgtga ttactattcg
    10261 tttgttttca agtttcatgt gtaaataatt atttttttaa aaggtcacat ggaactcgca
    10321 tgtcatacct tcatctactt tggtgaactc ctgcgtacat gctcgtttca tgtttttgga
    10381 taaattcaac tgttataaat cagacattat ttttattttc aattttcaac tgttttctac
    10441 tattcgttga gttgctgttt tatttatcac tctgcaaaca tacaacattt aaacgaatta
    10501 attaatcatt tgattaatct atttgtttaa attatttgtt taaattttat taaaaacagc
    10561 taacgctctg aatatcaatt cagagcgtta gctgtaacct tatttcaaat gcagtattat
    10621 tatcgcagat gcttaaatgt gttacccaga ttaccggcta cgtcgcttgc cagcaagctt
    10681 tcttccgact gtgtctgcaa agccagatcg gctgataaac catgcaaata tacacccagc
    10741 acagcactat cggtcgatga ataaccctga gcaagcagac ctacaatgat tccggtgagc
    10801 acatcgccca ttccacccga tgccattccg ggatttccgg tggagttgaa atacattttt
    10861 ccgtcgggag
//
LOCUS       DAOPYB010000102        10863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_6952_length_10863_cov_9.799778, whole genome shotgun sequence.
ACCESSION   DAOPYB010000102 DAOPYB010000000
VERSION     DAOPYB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10863
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6952_length_10863_cov_9.799778"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..910
                     /locus_tag="PKH58_08530"
     CDS             107..910
                     /locus_tag="PKH58_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNX89113.1"
                     /translation="MRNNEISETAESKSLSIEIKKLSAEYLELYTQFKDMTDNEPLLY
                     SVYLNKLGYLLFELFEKKTECSRLKMKMELIQTALNRDEKPDIPVIEVKLNTLLSNYY
                     QQINKNAETLETAKQVLRDLLPPEFVAELKEIFHLLCKKLHPDLNPHQTENDKDLFLK
                     VKAAYDLNDLTELKKILLNLDSKQEPAEQTTQQTTGEKRKKLDFISKNIAVLKEKIEK
                     LNETFPYNMKDVLNDDDKVTAEQDAVKVQINFYEQEANKYEAFIELMLS"
     gene            913..1299
                     /locus_tag="PKH58_08535"
     CDS             913..1299
                     /locus_tag="PKH58_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HNX89114.1"
                     /translation="MNQSIIPISPSLLAMLSKGTSGVLPFQREIFLLEITIAGTSFST
                     GLQTVEQEIVPEKVLTMKREPDNKHDKFAIAIFVQDVRVGYVPAELNEVCSRLMDAGK
                     MFFCRVISREWKGNWLRIKANIYMVE"
     gene            complement(1306..1923)
                     /gene="lptC"
                     /locus_tag="PKH58_08540"
     CDS             complement(1306..1923)
                     /gene="lptC"
                     /locus_tag="PKH58_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNX89115.1"
                     /translation="MKFSKQIIINMTAAFWVVVVLFGAVACQNKKTEKIAAVKDRQAM
                     PGLHATKITTVISDSGITRYRIYTDQWDVFDKAKEKYWEFPKGLHFERFDENLVIDAN
                     FHSNYARYYEARQLWEFKGKVKAINMMGEMFESERLYWDRAQELIYSDTLVKITQLTR
                     VVSGYGFQSNQSMTKYSFRATLKGLLVVPDENSNAAALPAGAAHP"
     gene            complement(1944..3188)
                     /locus_tag="PKH58_08545"
     CDS             complement(1944..3188)
                     /locus_tag="PKH58_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89116.1"
                     /translation="MYNKKVVFAIALFFSAIQLTVGQNNTNSPYTRFGYGDVTQSTAT
                     ELRGMGGVSLGNRSKNTINTVNPASYSSVDSLTFMFDIAGSARYSIFSDDDGSNKTFN
                     SNLEYLTMRFPLAKWLGFSAGLTPYSFVGYNFNQVDSLQMPVSQTQDAKYINYYTLFN
                     GAGGFTQVYGGLSANILNHISLGVNAYYMFGEVNNNRNLVFSSTTAYQSVAYANSISA
                     NDFRFRYGIQLYNTFAEKHDVVLGAIYEGKTKFNARFSAIANDSVLNSANGFELPQTL
                     GIGLNYTYNKQLTVGIDVTQQAWAKASFMNKTDSLVNTTKIALGAEYVPNPVGRRYTD
                     RIRYRVGFNTVNPYYTAGTTKLPNNYTVSFGVGFPTKTSKSILNASLEYGKMGSSLLK
                     ENYLKLTFSASINEFWFVKPKL"
     gene            complement(3336..4187)
                     /gene="hisG"
                     /locus_tag="PKH58_08550"
     CDS             complement(3336..4187)
                     /gene="hisG"
                     /locus_tag="PKH58_08550"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNX89117.1"
                     /translation="MLRIAIQSKGRLNEECMTLLNEAGIKLEWGKRLLLVPAKNFPVE
                     VLFLRDDDIPQTVADGVADIGIVGENEFAEKNKKVNIEKRLGFSKCRLSLAIPKSADY
                     QGLSWFNGKVIATSYPVILDNFLKQNNISADIHVITGSVEIATGIGLADAIFDIVSSG
                     STLLTNQLQEVEVVMKSEALLISNPDLSPVKREVLDDLLFRLEAVQVAEDKKYVLMNI
                     PTANLSQIVQVIPGMRSPTIMPLAKDGWSSLHAVIGEKEFWDIIGKLKNLGAEGILVI
                     PIEKMIL"
     gene            complement(4359..4544)
                     /locus_tag="PKH58_08555"
     CDS             complement(4359..4544)
                     /locus_tag="PKH58_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89118.1"
                     /translation="MKKVILFFAVAAVFAACTPKQAEQTEQNIDSVATEVVDTTAAAV
                     DTAATAVVDSAATAVVK"
     gene            complement(4791..4961)
                     /locus_tag="PKH58_08560"
     CDS             complement(4791..4961)
                     /locus_tag="PKH58_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89119.1"
                     /translation="MKKVFLFIAVAFAIAACAPKQAEEKKVQEDSVVVVDTVAAPADT
                     AIASVDSVAPQM"
     gene            complement(5125..6849)
                     /locus_tag="PKH58_08565"
     CDS             complement(5125..6849)
                     /locus_tag="PKH58_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX89120.1"
                     /translation="MIKENFIRLYEDAFRRNWELPGFTDYGENHTMTYEQIAVSIAKL
                     HLLFEQCDVKQNDKIALCGKNNSHWAVVYLGAVTYGAIVVPILQDFNPNDIHHVVNHS
                     ESKLLFVSDYIWENLEEENFISLQAVFSLNDYRTLVLLNQPVPTLLEENESKESIIAP
                     LSKAKLSKRHIDELFAHKYPYGFNKDSVRYVDKSNSEIVSINYTSGTTGFSKGVMTSG
                     NALAGNVTFAVNTKLIAPGFNIVAFLPLAHAYGCAFDFLASTCAGCHIHFIGRTPSPK
                     ILLKAFAEVRPNVIFCVPLIIEKIYKKQIQPLLARPSVRWVLSIPFLDQPVLAQIRKK
                     LVDAFGGNFSQIVVGGAPLNAEVEEFFTKIKFPFTVGYGMTECAPLISYTEAKDFISK
                     SSGKILDGIMEVKIDRPDPNTGVGEIFVRGENVMSGYYKNPEATEQILDADGWLHTGD
                     LGTVDADGNVFIRGRNKTMILSANGQNIYPEELESKLNNMPFVMESLVIDSNGKLIAL
                     VCPDYDAVDAEGLDQKGLEDVMEENRKLLNSLGASYESVSKIQLYPHEFEKTPKKSIK
                     RYLYTTGF"
     gene            7143..9605
                     /gene="pheT"
                     /locus_tag="PKH58_08570"
     CDS             7143..9605
                     /gene="pheT"
                     /locus_tag="PKH58_08570"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNX89121.1"
                     /translation="MNISYNWLKNYININVSPEQISKILTSIGLEVGSVEQIQTVKGG
                     LEGLVVGEVFSCAKHENSDHLHVTTVNVGQTEPLQIVCGAPNVDAGQKVIVATVGTKL
                     YSGDDCFTIKRSKIRGVESFGMICAEDEIGIGTDHNGIMVLPEDTEVGMLAKDYFGIK
                     DDTLIEVDITPNRIDAASHYGVARDLAAYFALIDNSVKLTKPSVDTFRVENTSLSIPV
                     VIENTAACPRYSAITISGVTVTESPKWLKDNLLAIGLRPINNIVDITNFILHELGQPL
                     HAFDADKITTGEVRVKNLPEGTPFTTLDGVERKLSSEDLMICNGDEPMCIGGVFGGLN
                     SGVTEATRNVFLESAYFNPVSIRKTARRHGLNTDASFRFERGCDPEITIYALKRAALL
                     IQEIAGGTISCEPVDEYPVQSHPFDVTLNKEKIDSLIGKKIGRDNIVKILNGLEIEIV
                     SENEEQFQLKVPAYRVDVQRDVDVIEDILRIYGYNNVEIGDKLVSTLSYASKPDSHKL
                     QILISEQLTAQGFNEVLNNSLTKGSYYNGLTAYPETESVKLLNPLSSDLNVMRQTLLF
                     GGLENIARNINYRNSDLKLYEFGNCYHYHNENKNPENPLDAYTEEYHLGMWITGNKFS
                     DSWTSGTEKSSFYELKAYVQNILTRFGLNLRTLKTEEFADDLFAEALRISSAKGTLLV
                     TYGIVNPEMRKRTDVNAEVYYADLVWNNILSELKDSAVKYSELPKYPEVRRDLAFLLD
                     KNTTFAEIEKIAYESERKFLKKVSLFDVYEGKNLGAGKKSYAVCFILQDESKTMTDNV
                     IDGIMRKIQQNIETKLNAKLRS"
     gene            complement(10296..>10863)
                     /locus_tag="PKH58_08575"
     CDS             complement(10296..>10863)
                     /locus_tag="PKH58_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89122.1"
                     /translation="LFDFMTNKQLISNHEKSNQCHFVDAVFNLYKGNYEIGLENIHKY
                     LSTAIKNSNALYVQMRLNYMLNRWNDADKLIEEICEMENQMIPKVYFRGGIINYCFLD
                     LPGIGILQTIIKQYPKSIETYEYLQQFCREKNLALPENLESNNEIITEFNKDPFNPKF
                     IKVLTLNMDQNNEIFQDFRNALLNEVFE"
BASE COUNT         3376 a         2261 c         2023 g         3203 t
ORIGIN      
        1 taaatattta agcagataaa aacacttaat gattaatttg aaacttaatg cttatctttg
       61 attgtggaaa gcctggaaat taatcaataa taaaaaacag cctgtcatga ggaataatga
      121 gatatccgaa acagccgaaa gtaagtctct ttcgattgaa ataaaaaaac tcagcgcgga
      181 atacctcgaa ttgtacactc agttcaaaga tatgaccgat aatgagccgc ttctttattc
      241 ggtgtacctg aataaactcg gatatctgct cttcgaactt tttgaaaaga aaacggaatg
      301 ttcgcgactg aaaatgaaga tggagctgat acagaccgct cttaaccgcg acgagaaacc
      361 ggacatcccc gttatcgaag ttaagctaaa cacgttgctt tccaattatt atcaacaaat
      421 aaataaaaat gctgaaaccc tcgaaacagc caaacaggta ctgagagatc tgcttccacc
      481 cgaatttgtg gcagaactga aagaaatatt ccaccttctt tgcaaaaaac tacatccaga
      541 tctaaatccg catcaaactg aaaacgacaa agatttattt ctgaaagtaa aagctgccta
      601 cgatctgaac gacctgacag aactgaagaa aatacttctc aatctggaca gcaaacagga
      661 acctgctgaa cagacaactc agcaaaccac gggggagaaa cgcaaaaagc tggatttcat
      721 tagtaaaaat atagctgtcc tgaaagagaa aatagagaaa ctgaacgaaa cttttcctta
      781 caatatgaaa gatgtcctta acgacgatga taaagtgact gctgaacagg acgctgtcaa
      841 agttcagatt aatttttacg aacaggaagc aaataaatac gaagccttca ttgaattaat
      901 gctcagctga ctatgaatca atcaattatt ccgatttcac ccagtctgct tgcaatgctc
      961 agcaaaggaa catcgggtgt actgccgttt caacgggaaa tattcctgct ggaaataaca
     1021 atcgcgggta ccagttttag taccggactt cagactgtgg agcaggaaat tgtaccggaa
     1081 aaagtgctca ccatgaaacg ggagccggat aacaagcacg acaagtttgc cattgccatt
     1141 tttgtacagg atgtacgcgt cgggtatgtg cctgctgaac tcaacgaagt atgttcgcga
     1201 ctgatggatg ccggtaaaat gttcttctgc agggtaatat ccagggaatg gaaagggaac
     1261 tggcttcgaa tcaaagccaa tatttacatg gtagaataat cagtcttacg gatgcgcggc
     1321 accggcaggt aatgctgccg cattgctatt ttcgtcggga acaacaagca atcctttcag
     1381 cgttgcccgg aaactgtatt tggtcatcga ctggttcgat tgaaatccat aaccggaaac
     1441 aacacgtgtc agctgtgtaa ttttcaccag cgtatccgaa tatatgagtt cctgagccct
     1501 gtcccagtac agtcgttcac tttcaaacat ttcacccatc atgttgatgg ctttaacttt
     1561 acccttaaat tcccataatt gtctggcttc gtagtagcgg gcatagttgc tgtgaaagtt
     1621 ggcatcgatt accaaatttt catcaaaacg ctcgaaatgc agcccttttg gaaattccca
     1681 gtatttttcc ttcgctttat cgaaaacatc ccactgatca gtataaatcc ggtaacgtgt
     1741 gatacccgag tcggatatta cagttgttat tttggtcgcg tgaagaccgg gcattgcctg
     1801 cctgtctttt acggctgcaa tcttctctgt ttttttattc tgacacgcaa ccgcaccaaa
     1861 taacacgaca accacccaaa aggcggctgt catattgatg attatttgct ttgaaaactt
     1921 cattcttttt tggaaaagga tctctacaat tttggcttca caaaccagaa ttcatttatc
     1981 gaagcgctga aagtgagctt cagatagttc tccttcaaca gcgacgaacc cattttaccg
     2041 tactcgagcg aagcgttcag gatagatttt gaggtttttg tcgggaaacc aactccaaag
     2101 ctgacggtgt aattattagg taatttggtg gttcctgctg tatagtaagg atttaccgta
     2161 ttgaaaccaa cgcgataacg tattctgtct gtatatcttc ttccaaccgg attaggaaca
     2221 tattcagctc cgagagctat tttggttgta ttcaccagcg aatcggtttt gttcataaac
     2281 gaggctttcg cccatgcctg ctgtgttacg tcaataccta cagtcagttg cttgttgtac
     2341 gtgtagttta atccaattcc cagcgtttgc ggcagttcaa atccgttggc agaattaaga
     2401 accgaatcat tggctattgc cgagaacctg gcattgaact tggttttccc ttcataaatc
     2461 gctcctaaca ccacatcgtg cttttctgca aatgtattat acagctgaat accatagcga
     2521 aaccgaaaat cattagcact gatgctattg gcataagcta ctgactgata ggcagttgtt
     2581 gaagaaaaaa ccaggtttcg gttattattt acctccccaa acatatagta agcatttaca
     2641 cctaacgaaa tgtggttcag aatgttggcc gacaaccctc cgtaaacctg ggtaaatccg
     2701 ccggcgccgt tgaaaagagt ataataattg atatatttag catcctgtgt ctgactgaca
     2761 ggcatttgca gggagtcaac ctggttaaag ttatatccta cgaaagaata tggtgtcaga
     2821 cccgcgctaa acccaagcca tttcgccagg ggaaaacgca tcgtcaggta ctccaggttg
     2881 gagttaaaag ttttattcga gccatcatca tccgagaaaa tcgaatatct ggcgctaccg
     2941 gcaatatcaa acatgaatgt aagtgaatcc acactgctgt atgaagccgg atttacggta
     3001 ttgattgtat ttttggatct gttaccgagc gaaacgcccc ccatcccccg cagctctgta
     3061 gctgtgcttt gcgttacgtc tccgtatccg aaacgtgtat agggcgaatt tgtattattc
     3121 tgacctacag tcaactgaat ggctgaaaaa aacaaggcga ttgcaaaaac tacttttttg
     3181 ttatacattc aatttatttt tccggtttac ccgttttaac ttaattttta ttggtaatgc
     3241 aaagatggtg taattaattt tcctgtcaaa gtaaataaag ttgtaccccg ttaaaaaaac
     3301 tgaaacagag ggttattatt ctattttcag gtgttttata aaatcatttt ttcgattgga
     3361 ataaccagaa ttccttcggc tcccagattt ttcagttttc cgattatatc ccagaattcc
     3421 ttttcaccaa ttaccgcatg caacgaactc catccgtcct ttgccagtgg catgattgtc
     3481 ggactacgca taccgggaat gacctgtaca atttgactta gattagccgt gggaatgttc
     3541 atcagcacat attttttgtc ttcagccacc tgaacagctt caagacgaaa taacaaatca
     3601 tccagaactt ctctttttac gggggacaaa tcaggatttg aaatcagcag tgcttccgat
     3661 ttcatgacaa cttccacttc ctgcaattga ttggtcagca gcgtacttcc ggaactgaca
     3721 atatcgaaaa tggcgtcggc cagtccaatt ccggttgcta tctcgaccga accggtgata
     3781 acgtgtatat cggcagagat attattttgt ttcaggaaat tgtcaagtat taccggataa
     3841 gaagtggcaa tcactttccc gttaaaccag ctcaatccct ggtaatcggc agatttcgga
     3901 atagccagag acagtcggca tttactaaat ccaagtcttt tctcgatgtt taccttttta
     3961 tttttctctg caaattcatt ttctccgacg ataccgatat ctgcaactcc atcggctaca
     4021 gtttgtggta tgtcatcatc gcgaagaaaa agcacttcaa caggaaagtt ctttgccgga
     4081 acaaggagca gtcgtttgcc ccattcgagt ttaatgcctg cttcattcaa taaagtcatg
     4141 cattcttcat tcaaacgtcc tttggattgt attgctattc gtaacatggt tcgatcttgt
     4201 atttgttgga ttttccataa aacaagccgt gcattcaatg aaataataca ctgcgacttc
     4261 taagccaaca aaagtactac aattctgaca aaaaaaaaag tccgttagaa actaacggac
     4321 ttgcagatgt cttaataaat tttttgcgat agctaaaatt atttaacaac agcagtagca
     4381 gctgaatcaa caacagcagt agcagctgta tcaacagcag cagcagtagt atcaacaact
     4441 tcagtagcaa ctgaatcgat gttttgttca gtttgttcag cttgtttagg agtacatgca
     4501 gcaaatactg cagctacagc aaaaaataaa attacttttt tcattttgtt ttaattctta
     4561 aaaatttaac atgttagata ttttgttcaa ttcaaaatcg tgacaaaggt atatctttat
     4621 tttgatatac aattaatatt tctgactttt ttttattttt ttttcacttt tcggtgattt
     4681 tctttgaaaa aagcctaata agcgggtaaa aaacaaacat ttacagtttc aaattcaaat
     4741 tctgtttctt taaaacagaa aaactaacag caagcaggaa ttatcctttt ttacatctga
     4801 ggtgctacgc tgtcaacgct tgcgatagca gtatcggccg gagcagcaac ggtatcaact
     4861 accacaactg aatcttcctg tacttttttc tcttctgcct gtttaggggc acaagctgca
     4921 atagcgaaag cgacagcaat aaataaaaat acttttttca tctttctaat aaattgtaac
     4981 tattttatct ttgagtatca aaaacggtgc aaagttacta caattttata atctctgaca
     5041 aataattttt cgcttttttt ataaatattt tcactttaac ttttcggaac gatcaattaa
     5101 atgaatatca accacttacg aagtttaaaa tccggtagta taaaggtaac gcttaatgct
     5161 ttttttaggt gttttttcga actcatgcgg atatagttgg atttttgaaa cactctcgta
     5221 ggatgcacca agcgaattta acaactttct gttttcttcc attacatctt ccagcccttt
     5281 ctgatccaaa ccttcggcat caacagcatc ataatcagga caaaccagag caatcagttt
     5341 tccgttgctg tcaattacca gactttccat aacaaagggc atgttgttca gtttcgattc
     5401 gagttcttcg ggatatatat tctggccgtt agcactcagg atcatagttt tattacgacc
     5461 gcggataaac acattgccgt cagcatcaac ggtccccaga tcgccggtat gaagccagcc
     5521 gtcagcatcc agtatctgct cggttgcttc agggtttttg taataaccgg acataacatt
     5581 ctctccgcgt acgaatatct ctccaactcc tgtatttgga tcgggacggt caattttcac
     5641 ctccataatg ccatccagta tctttcccga tgatttggag ataaaatctt tagcctcagt
     5701 atagctgatc aacggagcac attcggtcat gccatagcca accgtaaacg gaaattttat
     5761 ttttgtgaaa aattcttcca cttcggcatt cagaggagct cctccaacca caatctgtga
     5821 aaaattacct ccgaatgcat ccaccagttt tttacgtatc tgggcaagca ccggctgatc
     5881 aagaaacgga atacttaaca cccagcgtac tgaaggacga gccagaaggg gttgaatttg
     5941 ttttttataa attttttcga tgatcagcgg cacacaaaat atcacattcg gacgtacctc
     6001 ggcaaaagct ttcagtaata tttttggtga aggcgtacga ccaatgaaat gaatgtgaca
     6061 tccggcacat gttgacgcca gaaaatcaaa agcgcagcca taagcgtgag caagcggtaa
     6121 aaaagcaaca atattaaatc cgggtgcaat gagtttggtg ttgacggcaa aagtgacatt
     6181 tcctgccaat gcattccccg aagtcataac tcccttacta aacccggtgg ttccggatgt
     6241 ataattaata gaaacaattt ccgaattcga tttatcgaca taacgaaccg agtccttatt
     6301 gaaaccgtag ggatatttat gagcaaacaa ttcatcgata tgacgtttgg acaattttgc
     6361 cttactgagg ggagcaataa tactttcttt gctttcattt tcttcgagca atgtggggac
     6421 tggctgattg agtaaaacca gtgtcctgta atcattcagc gaaaatacag cctgtaacga
     6481 aatgaaattt tcttcttcaa gattttccca gatgtaatcg ctgacaaata ataatttcga
     6541 ttcagaatga ttaacaacgt gatgaatatc attcggatta aaatcctgca gtatcggtac
     6601 aacgattgct ccgtatgtta cggcacccag atatacgact gcccaatgtg aattattctt
     6661 accgcacaat gcaattttat cattctgttt cacatcacat tgctcgaaaa gcaaatgtaa
     6721 ttttgcaatg ctgacggcaa tctgttcata cgtcatggta tgattttcgc cataatcggt
     6781 aaagccgggc aattcccagt tccggcgaaa agcatcttcg tacaaacgga taaaattttc
     6841 tttaatcata actgaactac tataaaataa aataattgtt gctaatgttt ttcatgaatc
     6901 tgaaactcaa taaaaaagtg atttcatgag tttcatctca ttttctcgac ctgcaaaagt
     6961 aactaaaaca cacaaaacaa ccaaaagtgt gcattttttt aatgataatt cgcaattaga
     7021 atattattca tttttcaaaa aagtaaaaaa acagtatctt tgcagtttat ataaccgtat
     7081 atcaaaacaa atttaagttt atccattatc ctctctgata aactgataca atattatata
     7141 gtatgaatat ttcttataac tggcttaaaa attacattaa tattaatgta tcgcctgaac
     7201 agatttcgaa aatactaacc tcaatcgggc tggaagttgg tagtgtagag caaattcaga
     7261 ccgtaaaagg cggactggaa ggtcttgtgg tgggcgaagt gtttagctgt gccaaacacg
     7321 aaaattcgga tcatctgcat gtaacaacag ttaacgtagg tcagaccgaa ccgctccaga
     7381 ttgtatgtgg tgctccaaat gttgatgccg gtcagaaagt gattgtggca acggttggta
     7441 caaaattata ttccggcgat gattgcttta ccatcaaaag gtcgaaaatc cgcggtgtcg
     7501 aatcatttgg aatgatatgc gctgaagatg aaatcggcat aggaacagat cacaacggta
     7561 ttatggtttt acccgaagac acagaagtag gtatgctcgc caaagactat ttcggtatca
     7621 aggacgacac gctgattgaa gtggacatta cccccaaccg cattgacgca gcatcccatt
     7681 atggtgttgc acgcgacctg gctgcatatt ttgccctgat tgataacagc gtgaaactaa
     7741 ccaaaccatc ggtcgatact ttcagggttg aaaatacaag cctgtccatc ccggtggtaa
     7801 ttgaaaacac tgctgcctgt cccagatatt cagccatcac catttcgggc gttaccgtga
     7861 cagaatctcc aaaatggctc aaagacaatc tgctggccat agggcttcgt cctatcaata
     7921 atattgtaga tataaccaac tttatactcc atgagctggg acaaccgctt catgcttttg
     7981 atgccgataa aatcaccaca ggtgaagtgc gcgttaaaaa cctgcccgaa ggaacaccgt
     8041 tcaccaccct tgatggcgtg gaacgcaaac tcagctccga agatctgatg atttgcaacg
     8101 gtgatgaacc gatgtgtatc ggaggcgttt tcggaggatt gaactccggt gtaacggaag
     8161 ctacccggaa tgtatttctc gaaagtgcat acttcaatcc ggtaagcatc cgcaaaactg
     8221 cccgccgtca cggactgaac accgacgctt cgttccgctt cgagcgcggt tgcgaccctg
     8281 aaatcacaat ctatgccctg aaacgcgcag cattgttgat tcaggaaatt gccggaggaa
     8341 ctatttcatg cgaacctgtg gacgaatacc ccgtacagtc acatccattc gatgttacgc
     8401 tgaacaaaga aaaaattgac agcctgatcg gtaaaaaaat cggaagagat aatattgtga
     8461 aaattctgaa cggactggaa atagaaatcg tatcggaaaa tgaagaacag tttcagctga
     8521 aagtaccggc ataccgcgtt gacgtgcaac gtgacgttga tgtaatcgag gatatcctac
     8581 gcatttacgg ctacaacaat gttgaaatag gtgataaact ggtttccaca ctaagctacg
     8641 cctcaaaacc ggacagccac aaactacaga tacttatctc cgagcaactt actgcacagg
     8701 gtttcaacga agtgctcaac aactcactga ccaaaggaag ttactacaac ggtcttactg
     8761 cttatcccga aactgaaagc gtaaaattat tgaacccgtt aagctccgac ctgaatgtga
     8821 tgcgtcagac tttattgttt ggaggacttg aaaacattgc ccgcaacatc aattaccgaa
     8881 attccgatct taaactgtac gaattcggca attgctatca ttatcataac gagaataaaa
     8941 atccggagaa tccgctggat gcttacaccg aagaatatca cctcgggatg tggattaccg
     9001 gcaataaatt cagcgattcg tggacaagtg gtaccgaaaa atcgagtttc tacgaactga
     9061 aggcatacgt tcaaaacata ctaacacgtt ttggattaaa tctgcgaacg ctgaaaacag
     9121 aagaattcgc cgacgattta tttgcagaag cactgagaat aagttcagcg aaaggcacct
     9181 tgctggtaac ctacggaatt gtaaatcccg aaatgcgcaa aagaacagat gttaatgcag
     9241 aagtgtatta tgccgacctg gtttggaata atatcctttc ggaactgaaa gattccgcag
     9301 ttaaatactc agaattgccc aaatatcccg aagtgcgtcg cgatctggca ttcctgctcg
     9361 acaaaaacac cacgtttgcc gaaatagaaa aaatcgccta cgaaagcgag cgtaagttcc
     9421 tgaaaaaggt aagtctgttt gacgtgtatg aaggtaaaaa tctgggagca ggcaagaaat
     9481 catacgctgt atgcttcatc cttcaggacg aaagcaaaac catgaccgac aatgtgattg
     9541 acggaataat gcgtaaaata cagcaaaata tcgagactaa actgaatgct aaactgagaa
     9601 gctaaaagac acaataattc aaatgcggga tttgctacaa gtcccgcatt ttttttgttt
     9661 acattttgat tcttttcata attttcttcc cgtgtcttat agaagtttgc cattaaatca
     9721 ataattaaac tgaaatacta tacctgattt tttcattctg acatactcca aacttattta
     9781 cagttattca aaacaccttt cccattaaaa atctgatttt ataaacaaaa aaatcatcct
     9841 atcgaagact gtttttcttc tcattttgac cgttttcagt tctcatttta ccatcttttt
     9901 atcagttttt tcctgtggtg agtgttaccg tcgtatccct atgcctctct cccctttttc
     9961 gggctgttaa ctgttgccgg gtcgattgct ctcacatctg gttgttaata cacaaatcac
    10021 cacaaacgct ctgtttaccg attctttcgt tttttccggg acaatttaac atctccgttt
    10081 tccgttgggt aacaaagttc gggacaaaac gatgattccg agattttgtc ataccaaagt
    10141 ccgggacaag actatgatcc ctatattttt tgccatatca aataactgcc gtacattagc
    10201 cttaaatcca atatatttca aaatttgtaa aataaattcc aagattcaaa attattgaac
    10261 cttggaattt aactcaagaa ataaacaaac agaaactatt caaaaacctc atttagtaaa
    10321 gcatttcgaa aatcctggaa aatttcatta ttttgatcca tatttaaagt taatacttta
    10381 ataaatttcg gattaaatgg atctttgttg aattcagtta taatttcatt atttgattct
    10441 aagttttctg gcaaggctaa attcttttct ctacaaaact gctgtagata ttcatatgtt
    10501 tcaattgatt ttggatactg tttgataatt gtttgcaaga ttccaattcc gggtaaatca
    10561 agaaaacagt aattgataat acctcctctg aaataaactt taggaatcat ttgattttcc
    10621 atctcgcata tttcctctat tagtttatca gcatcattcc atctgttcaa catataattt
    10681 aatctcattt gaacatataa tgcatttgaa tttttgatag cagtagataa atatttatgt
    10741 atattctcta atcctatttc ataatttcct ttatataagt taaatactgc atctacaaaa
    10801 tggcattgat tagatttttc gtgatttgaa atcaattgct tattagtcat gaaatcaaat
    10861 aga
//
LOCUS       DAOPYB010000103        10732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_7081_length_10732_cov_8.962911, whole genome shotgun sequence.
ACCESSION   DAOPYB010000103 DAOPYB010000000
VERSION     DAOPYB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10732
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7081_length_10732_cov_8.962911"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="PKH58_08580"
     CDS             complement(<1..1900)
                     /locus_tag="PKH58_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX89123.1"
                     /translation="MAKKLILTILIAINCGMSQAQHTLEFTLSDKLFYQGKELYDQRK
                     YAASYRNFEDFLKTTAATDAGMIVEAEYYLCSNLFEMRQKNAKLQLQDYLERHPYSPY
                     ADRTNYMLGMLEYEAKNYPAALNYFRKVDETHLINREKTDFLFCRAYSNLATEHPEKA
                     LDIFNTLKNQDDRYQVTAQYYAGYCLYLLGNYDAALPDLLAVEHNPEFEDVAPYYITQ
                     IYYSKKEYNEVEQRAEKLLKKYPDNKNNAEIYRILGEKAYADRNFSKAIDNLKKYEKS
                     APKVLRNDMYYLGVSCLKAGNPLEAVTYLSKVTIGEDEMSESAYLQLGNAYVKVGDMT
                     NAKMAFESATRTRFNAGVREEALFNYALSTYETSSAFGESVKAFEQFISEFPNSGKTD
                     DAFDYLSTVYLTSKDYQSAYKSISKIKSLTPKLRETKQYLEYQLGTEAFAANNYTKAT
                     DYFSKALQSAAQGKYLTDVYYWRAESYYRTGEFSKSVSDLQSFFNQPNAQKNQNYTQA
                     FYGMGYANFKQKKYSEALNWFLKYIREEKNTSAPAYADALNRIGDGYFTARNFTKATE
                     YYDKATEISPYNGDYAIFQSAYTDGLQKNYSKKINKLEQLLIKYPRSEYGDDALYEIA
                     RAWLMLENN"
     gene            complement(1934..2479)
                     /locus_tag="PKH58_08585"
     CDS             complement(1934..2479)
                     /locus_tag="PKH58_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNX89124.1"
                     /translation="MNFSKLFHFCPVCGSKEFAQKNVKSNECASCGFIYYINPSAATA
                     AFIRNEDGDLLVCRRAHEPAKGTLDLPGGFIDYDETAEEGVAREILEETGLSAINVRY
                     LFSLPNDYKYSGLDIPTMDLFFECKLENYSRLSPADDVVECFFLPLGEINPALFGLNS
                     IKKAVGMYIDNHNMKKEIFLA"
     gene            2671..4065
                     /locus_tag="PKH58_08590"
     CDS             2671..4065
                     /locus_tag="PKH58_08590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX89125.1"
                     /translation="MSGNFSFQLDDEAGELVSRYEKYLLGTAPGYFDVDEMERIVEYY
                     LRNGQTKDSLNALELGKKLHPSSGALDAKRAKIYLATGDLKKAFRLLSNLVENSDPEV
                     EQLKIEALIKLERLQEAKSLALSLIESESEEVDQFCIEMALKFMAESQFDYACDILLI
                     GEKYNPDNVDLLFELAFCQEQKGMLTEAVSTYSRIIDIDAYIGEAWFNIGQIYFLLNE
                     FEEAIDAYDYALVINEKDSISQIQKAHAHFQLNQFGNAIEAYLAYSEMVTEKWQVMLF
                     VGESYEKLENFPKAYHYYKLSLDEMADNYDALIGISICLLEMEEYSQCLGYIEQAMKI
                     NDGMSDAWVYLAEAYIGLDNIDEALKAYLKAISIEPNQADTLLAIGNIYMDNAEYQTA
                     LRYYETAYSFDTTLENIELFLAVANYYNGNYNVSADYLLQAVNRNLDASDLFLELCPG
                     AAEKLSTNHSTENQ"
     gene            4062..4799
                     /locus_tag="PKH58_08595"
     CDS             4062..4799
                     /locus_tag="PKH58_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNX89126.1"
                     /translation="MRFFGLIGYPLGHSFSKKFFTEKFDKEGINARYDLYELREIEEF
                     AGLKNMTDLCGLNVTIPYKQQIIPFLDELDPVATEIGAVNVIKFIRQNNELKLKGFNS
                     DAVGFMQSIQPYLRPHHRKALILGTGGASKAIDYSLRMMGVETKFVSRSPVNGQFSYH
                     DLNEDILTEYHVIINASPVGTFPHVDSCPDIPYRFLTSGHLLFDSVYNPAETLFLQKG
                     KAQGTSVLNGEGMLTGQAEAAWKIWNE"
     gene            4854..5552
                     /locus_tag="PKH58_08600"
     CDS             4854..5552
                     /locus_tag="PKH58_08600"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNX89127.1"
                     /translation="MNLFYAPAIHTEQVLPEEESAHAVRVLRLQAGSTIEVVDGAGGF
                     YKAVITNPHPKHCTFEIVDKIDEYGKRNYKLHIAIAPTKNIERYEWFIEKAVEMGIDE
                     ITPLLCRYSERKIIKPERLEKIIVSAAKQSIKAFFPKLNTMCSFDDFINTKQAAQCFI
                     AHCYDEEKHLLKNLYHKSSDVIILIGPEGDFSQEEVKKAIAKGYKPVSLGSSRLRTET
                     AGLAACHTVVMMNE"
     gene            complement(5611..6807)
                     /locus_tag="PKH58_08605"
     CDS             complement(5611..6807)
                     /locus_tag="PKH58_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX89128.1"
                     /translation="MKKLLFILISAALLVSCQNEKLSDLSKVATEVPEVSSQIIPGKY
                     VVVYNTIGIKKAARIASLSGNSIITPENVVTMTKEILSSSNIQMRAPELAYATALKGV
                     AIELTDAEAAELAKSPAVKGVYPDMMVNFGLPEVSINGKPATPAAQSTPWGITRIGGS
                     TDGTGKTAWIIDTGIDMDHPDLKVDQTKGKNFVTTTATPDDDNGHGTHCAGIVAALNN
                     GQGVIGVAAGATVVPVKVLNKRGSGAYSAIIAGVDYVAANAKAGDAVNMSLGGPVYTP
                     LDDAIIAMAAKGIKVAIAAGNESQDTNNVSPGHINGTNIYTISAMGTGDLWASYSNFS
                     NPPIDFCAPGSSIYSTYKGGAYATLSGTSMATPHVCGLLLLGNIGTDGYVKNDPDGNA
                     DPIAHK"
     gene            complement(7001..9301)
                     /locus_tag="PKH58_08610"
     CDS             complement(7001..9301)
                     /locus_tag="PKH58_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNX89129.1"
                     /translation="MRTLIILISLLFTMTVNLKAADKKPLYKDPNAPIEKRIDDLLSR
                     MTLEEKVGQMNQFVGIEHIKSAVANLSEEELKTNTASAFYPGFTHKDVEEWTKNGLIG
                     SFLHVLTLREANYLQSLAMQSKLQIPILFGIDAIHGNANAPDNTVYPTNIGLASSFDT
                     EMAYKIARQTAKEMRAMNMHWTFNPNVEVARDQRWGRVGETYGEDPYLVSRLGEQTVK
                     GYQGNLNSNEDVLACVKHFVGGSEPLNGTNGAPADLSERTIREVFFPPFKAGADAGAM
                     SFMTAHNELNGIPCHANEWLMNDVLRNEWGFKGFIVSDWMDIEHMYDLHGTAENLKEA
                     FYQSIMAGMDMHMHGIYWNELVTELVKEGRIPESRIDQSVRRILDIKFRLGLFENPFA
                     DEKQSMKVRLNPEHRATALEASRNSIVLLKNNGILPLKNQYKNVLITGINANDMNILG
                     DWSAVQKEENVITILEGLRMISPETSFDFVDQGWDPRNMDPKKVEEAVAKAQKSDLNI
                     VVAGDYMMRFRWKERTSGEDTDRSDLDLVGLQNELIEKISASGKPTILILVSGKPLSI
                     EKQASQAAAVIEAWEPGMYGGQAVAEIIYGKINPSAKLPVSIPRSVGQLQMIYNHKPS
                     QYFHPYVITSSEPLFPFGYGLSYTTYKYSSLKLSETTIPQNGNVNVSLDVTNSGTCDG
                     VEIVQLYIRDRFSQVTRPVKELKDFARLSLKAGETKTVTFTITPDKLMFLDKKLNPVV
                     EPGEFIVMVGSSSRDSDLLKQSFFVK"
     gene            complement(9312..10667)
                     /locus_tag="PKH58_08615"
     CDS             complement(9312..10667)
                     /locus_tag="PKH58_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNX89130.1"
                     /translation="MPEILSDNMVLQQSTTVNIWGKASPNNIVKVQPSWMKMTTTVKT
                     DNKGNWLAKIQTPEATFTPQTLTFSDGETLTLKNILIGEVWLASGQSNMEMPLNGFWN
                     CPIQDANETIALSGQNKGIRFAVVPKTPAYEPQETCPGKWMLSNPNNAPIFSATAYHF
                     AQMLYNTLNVPVGIIVCSWGGSRVESWTNREILETYPDIDLTQKGIETEHEWKRPLLM
                     YNGMLKPLSNFTLKGFIWYQGESNVGKHDVYARRFATMVELWRKDWKNNNLPFYYAEI
                     APWIYGDGETGISGALLREAQFKAQSIIPNSGMISTNDLVEEYEKINIHPKNKTDVGK
                     RLAYLALNQTYELKSIAAHGPEYKSMEIRDGKIILSFSYAQDGFSRENDIRGFEICGS
                     DKIFIPASAKLDNNNLKIILSSDKIQNPIAARYCFRNFQPGNLANTRGLPVVPFRTDN
                     F"
BASE COUNT         2986 a         2213 c         2252 g         3281 t
ORIGIN      
        1 cattattttc gagcatgagc caggcgcgtg caatctcgta aagtgcatcg tcgccatatt
       61 ctgatcgtgg atatttaatc agcagttgtt ccagtttatt tatctttttg gaataattct
      121 tttgtaaacc atcggtataa gccgactgga aaatggcata atcgccgttg taaggcgaaa
      181 tttcggttgc tttatcgtag tattcagttg ctttggtaaa gtttcgtgcg gtgaaataac
      241 cgtcgccaat gcggttgaga gcatcagcat acgcgggtgc agatgtgttt ttttcttccc
      301 ggatgtattt cagaaaccag ttaagcgctt cgctgtattt tttttgtttg aaattagcat
      361 aacccattcc ataaaatgct tgcgtataat tctggttttt ttgagcattg ggctgattga
      421 agaatgactg caggtctgaa actgatttgg aaaattcacc tgtgcggtaa taactttcgg
      481 ccctccagta atacacatcg gttagatatt tgccttgagc ggcagactgt agggctttgc
      541 tgaaataatc agttgccttt gtatagttgt tggctgcaaa tgcttctgtt ccaagctgat
      601 attcgagata ttgctttgtt tcacgtagtt tgggtgtaag gctttttatt tttgaaatgg
      661 atttgtaggc tgactggtaa tcttttgaag tcagatatac ggtagatagg taatcaaatg
      721 catcatctgt ttttccggag tttggaaatt cgctgataaa ttgctcgaat gctttgaccg
      781 actcaccaaa agccgaactg gtttcgtagg tcgaaagtgc atagttgaag agtgcttcct
      841 ctcttacacc cgcattgaag cgggttcggg ttgcagattc aaaagccatc ttggcattcg
      901 tcatgtcgcc tactttaaca taggcattgc ccagttgcag ataagcgctt tcgctcattt
      961 catcctcacc gatggttact ttcgacaaat atgttaccgc ttccagagga ttgccggctt
     1021 tcagacaact tacaccgagg taatacatgt cattgcgcaa tacctttggg gctgatttct
     1081 catatttttt caggttatca atggctttgg agaaattgcg gtcagcatag gctttttcgc
     1141 ctaaaatccg gtatatttcc gcgttgtttt tattatcggg atatttcttc agcaattttt
     1201 ccgccctttg ctccacttca ttatactctt ttttggaata gtaaatctga gtgatatagt
     1261 agggcgccac gtcttcaaat tcgggattat gctcaacagc aaggaggtcg gggagggcgg
     1321 catcgtaatt gccaagcaga tagaggcagt agcctgcata atactgagct gtaacctgat
     1381 accggtcatc ctgatttttc agcgtattga aaatgtcgag tgctttttcg gggtgttcgg
     1441 ttgccagatt ggagtatgcc cgacagaaca gaaaatcggt tttttcgcgg ttaatcagat
     1501 gtgtttcgtc aactttccga aaataattca gcgctgcagg gtaatttttt gcttcatatt
     1561 ccagcatccc gagcatgtaa tttgtgcggt ctgcataagg actgtacgga tgtctttcca
     1621 gataatcctg tagttgtaat ttggcatttt tctgacgcat ttcgaagagg ttggaacata
     1681 aatagtattc agcttcgacg atcattccgg catcggtagc agccgttgtt ttcagaaaat
     1741 cttcaaaatt ccgatacgaa gcagcatatt tgcgttgatc atagagttct ttcccctgat
     1801 aaaataactt atcggacagt gtaaactcaa gcgtgtgctg agcctgcgac ataccgcagt
     1861 taattgcgat tagtatcgtt aaaatcagtt tttttgccat aattatgttg atgtagtgtt
     1921 ttaaaaaata atattatgcc aggaaaattt cttttttcat attatgatta tcaatataca
     1981 tgccgaccgc tttttttatt gaatttaaac cgaaaagtgc gggatttatt tcgccaagtg
     2041 gcagaaaaaa acattccacc acatcatcgg caggcgataa ccggctgtaa ttttctaatt
     2101 tacactcgaa aaacaagtcc atggtaggaa tatccagccc ggaatattta taatcgttag
     2161 gcagcgagaa taaatatctc acatttatgg cacttaaacc ggtttcttcc agtatctccc
     2221 gtgccacgcc ttcttcggct gtttcatcat aatcgataaa tcctcccggc aggtcgagcg
     2281 tccctttagc aggctcgtgg gctctgcgac aaaccagcaa atcgccatcc tcattacgaa
     2341 taaatgcagc tgttgcagcc gaaggattga tgtagtaaat aaaaccacag gaagcgcatt
     2401 catttgactt tacattcttc tgcgcaaact cttttgagcc gcaaaccgga caaaaatgga
     2461 ataattttga gaaattcata gacctgttta atgatttcta caaagatagt gaaatgtcag
     2521 aaaacgaaaa aaaaacagta agccacattc cacagtttac agcaacaatc ggaccttttc
     2581 ggacgaattt atttctaaat gtacataagt tttgtgaaaa tagcttcagt ttcgattaaa
     2641 atatagtatt tttgcaaaaa aagtaatgtt atgtcgggaa atttttcctt tcagcttgat
     2701 gacgaagcag gtgaattggt aagcagatat gaaaaatatc ttttgggtac tgctccgggc
     2761 tattttgatg tggacgaaat ggaacgtata gtggagtatt atcttcgtaa cggacaaacc
     2821 aaagactcac tcaacgctct ggaactgggg aaaaaactgc atccatcaag tggtgcactt
     2881 gatgccaaaa gagcaaaaat atacctggct acaggtgatc ttaaaaaagc ctttcgctta
     2941 ctgtcaaatc tggtggagaa ttccgatccg gaagttgaac agttaaaaat agaagccctg
     3001 attaaacttg aacgcttaca ggaagcaaaa tccctggcat tatcactcat cgaaagcgaa
     3061 agcgaggaag ttgaccagtt ttgtattgaa atggcgctga aatttatggc cgaatctcag
     3121 tttgattatg catgcgacat acttctgatc ggagaaaaat ataatcccga caatgtggat
     3181 ttgctattcg aactggcttt ttgccaggaa caaaaaggga tgcttaccga agctgtttca
     3241 acctattcgc ggattatcga tatagatgct tacattggtg aagcctggtt taatatcgga
     3301 caaatttatt ttctgctgaa tgagtttgaa gaggccattg atgcatatga ttatgccctg
     3361 gtaatcaacg aaaaagacag catttcacag atacagaaag ctcatgcaca ttttcagctc
     3421 aatcagtttg gaaatgccat agaagcttat ctggcttatt ccgaaatggt tacggaaaaa
     3481 tggcaggtca tgctttttgt gggtgagagt tacgaaaaac tcgaaaattt tccgaaagca
     3541 tatcactatt acaaactatc tcttgacgaa atggcagata attacgatgc tctgattggc
     3601 atttcaattt gtttgctcga gatggaagaa tactcacaat gcctgggtta catcgaacag
     3661 gcaatgaaaa tcaatgacgg tatgtcggat gcatgggtct atctggctga agcttacatc
     3721 ggactggata atattgatga agcgctcaaa gcctatctga aagcaatatc cattgaacct
     3781 aatcaggctg atacgctcct ggcaatcggt aatatatata tggataatgc tgagtatcag
     3841 acagccctta gatattacga aacagcctac agttttgaca ccaccctcga aaatattgaa
     3901 cttttccttg ctgtagccaa ttattataac ggcaattaca acgtatcggc cgattacctc
     3961 cttcaagctg taaaccgcaa tctggatgct tcagacttgt ttcttgaatt gtgtccggga
     4021 gctgccgaaa agttatcaac caatcattca acagaaaatc aatgagattt ttcggattaa
     4081 taggttatcc cttaggtcat tcgttttcaa aaaagttttt tactgaaaaa tttgacaagg
     4141 agggaattaa tgcccggtat gatttgtatg aattaaggga aatcgaagaa tttgccggac
     4201 tgaaaaatat gactgacctc tgcgggctga atgtaaccat tccctacaaa cagcaaatca
     4261 ttccttttct cgatgaactg gaccctgtcg ccactgaaat aggagctgtt aatgttataa
     4321 aatttatacg tcagaataac gaactgaaac tcaaaggctt caattcggat gctgttggat
     4381 ttatgcaatc catacaaccg tacctaaggc cacatcatcg aaaggcgttg atacttggta
     4441 ccggaggtgc atccaaagcg attgattaca gtttgcgaat gatgggtgtt gaaacgaaat
     4501 tcgtatcacg ttcgcctgtt aacggacagt ttagttatca tgacctgaat gaagatatac
     4561 tcactgaata tcatgttatt ataaatgctt ctccggtagg tacatttcct catgtggata
     4621 gctgtccgga tattccttac cggtttctta cttccggaca tttgcttttt gattcggtat
     4681 ataatcctgc agaaacatta tttcttcaaa aaggaaaagc gcagggaaca tcggtgctca
     4741 atggtgaagg catgcttacc gggcaggccg aagcagcatg gaaaatatgg aatgaataat
     4801 catcacacag ctatcattta ctcctaatta taaactgaaa aaattctact gcgatgaatc
     4861 tgttttatgc ccctgctatc cataccgaac aagtacttcc ggaggaagaa tcggcacatg
     4921 cagtgcgtgt actcaggttg caggctggta gtactatcga agtggtggat ggagccggag
     4981 gattttacaa agccgtgatt accaatccgc atcccaaaca ttgcactttt gaaattgtag
     5041 ataaaatcga tgagtacgga aaacggaatt ataaactcca tatcgccatt gcaccaacca
     5101 aaaacataga aagatacgaa tggtttattg aaaaggcagt agaaatggga attgatgaga
     5161 ttactccttt actttgccgg tattccgaac gtaaaatcat caaacccgaa aggcttgaaa
     5221 aaatcattgt atcagctgcc aaacagtcca ttaaggcttt tttccccaag ctgaatacaa
     5281 tgtgcagctt tgatgacttt atcaatacaa aacaggctgc tcagtgtttt attgctcact
     5341 gctacgatga agaaaaacat ctactcaaga acctgtacca taagtcatcg gatgtcatta
     5401 ttctgattgg ccctgaaggt gattttagtc aggaagaagt aaagaaagca atagctaagg
     5461 gatacaagcc cgtgtcgtta ggcagtagtc gtttacgaac cgagacagct ggtttggcag
     5521 cttgtcacac agtagtgatg atgaacgaat aaaaaatgcc cgatatattt aatatcgggc
     5581 attttcatta tacgttatat ccagaaacaa ttatttgtgt gcaatgggat cggcattacc
     5641 atccgggtca tttttcacat agccatctgt tccgatgttt ccaagtagta aaagtccgca
     5701 aacgtgaggt gttgccatcg aagtaccgct cagtgtagca taagcaccgc ctttgtaagt
     5761 tgagtaaatt gatgaaccag gggcgcagaa gtcaatcggc ggattgctga aattgctgta
     5821 ggaagcccac aagtcgcctg tgcccatcgc tgatatggta taaatatttg ttccattaat
     5881 atgtccgggt gaaacattat tcgtgtcttg agattcattg ccagctgcaa tggctacttt
     5941 aatgcctttt gcagccatag caataatagc atcgtcaagc ggagtataaa ccggtccacc
     6001 taaactcata ttcacagcat cgccggcttt ggcattagct gcaacataat caacacctgc
     6061 aatgatagcc gaataagctc cggaacctct tttattaagc actttgacag gaactacggt
     6121 tgcaccggca gcaacaccaa tgactccctg gccgttatta agagcggcaa caattccggc
     6181 acagtgagtt ccatgtccgt tatcgtcatc gggagtagcg gtggttgtaa caaaattttt
     6241 acccttagtc tgatctactt tcaaatccgg atgatccata tctataccgg tatcgatgat
     6301 ccaggctgtt ttacctgtac cgtctgtact tccacctata cgggttatcc cccacggagt
     6361 tgactgggca gccggagttg caggttttcc gtttattgat acttcgggca atccgaagtt
     6421 gaccatcata tccggataaa caccttttac agctggagac ttagccagtt cggctgcttc
     6481 agcatcagtc agctcaattg ctactccttt aagtgcagtt gcgtaagcta actcaggtgc
     6541 tctcatttga atgttcgagg aacttagaat ttccttagtc atagtcacta cattttcagg
     6601 agtaattatt gaatttcctg acagagaggc aattctggca gcttttttta taccaatagt
     6661 attgtaaaca acaacatact tacccggaat gatctgtgaa ctaacttcag gaacttccgt
     6721 tgcaactttg ctcaaatcgc tcagtttttc attttgacag gataccagca atgctgctga
     6781 tatcaagata aataatagtt tcttcattat tgtgggtcta ttgttaataa ataaaagaat
     6841 atattttgca aatataataa tatgtattta atgtcaaaat aatttttcta taataacaca
     6901 aaataatttc tttagtcaat ttccttatat taaaagatag aggtttctga attcagaaac
     6961 ctctatgacc aattaaaaaa atcacaacaa aatcaatcta ttatttcacg aaaaagcttt
     7021 gtttcagcaa atcgctatcc ctggaagatg aaccgaccat cacaataaac tcgcctggtt
     7081 caacaaccgg atttaatttt ttgtcaagaa acatcagttt atcaggtgta atggtgaatg
     7141 taaccgtttt tgtttcaccg gctttaagcg ataaccgagc aaaatctttc agttctttaa
     7201 ccggacgggt aacctgactg aatctgtcgc ggatatacaa ctgcacaatc tcaactccat
     7261 cacacgtacc gctgtttgtt acatccaaac tgacattaac attgccgttt tgaggaattg
     7321 tggtttcaga aagtttcagg ctgctgtatt tgtaagtagt gtaggagagt ccgtagccga
     7381 acggaaaaag cggttcgctc gatgtaatga catatgggtg aaaatactgt gaaggtttgt
     7441 gattgtaaat catctgtaac tgtcctacgc tgcgtggtat gctaaccggc agtttagctg
     7501 aaggattgat ttttccgtat ataatttcag caaccgcctg tcctccatac atgccaggtt
     7561 cccacgcttc tattacagca gcagcctgac tagcctgttt ttctatacta agtggctttc
     7621 cggacacaag tatcaaaatt gtaggtttac ctgaagctga aattttttca atcagttcat
     7681 tctgcaatcc taccagatca agatcggagc gatcggtgtc ttcgccgctt gtgcgctcct
     7741 tccagcggaa tcgcatcatg taatcacctg caaccacgat attcagatcc gatttttgag
     7801 cttttgcaac ggcttcctcc acttttttcg gatccatatt gcggggatcc catccctgat
     7861 cgacaaagtc aaaacttgtt tcgggcgaaa tcattctcag cccttcgagg attgtaatca
     7921 cattttcttc tttctgaaca gcgctccagt cgcccagaat attcatatcg ttggcattga
     7981 tgcctgtaat gagaacattc ttatattggt ttttcagtgg aagtattcca ttgtttttca
     8041 gaagaactat actattgcgt gacgcttcaa gggcagtagc ccggtgttcg ggattcaggc
     8101 gaactttcat cgactgcttt tcgtcggcaa acggattttc gaacaatccg aggcggaatt
     8161 taatatccag tatgcgtctc actgactggt cgatacgtga ttccggaatt cgtccttctt
     8221 tcacaagttc agttaccagt tcattccagt aaatgccgtg catatgcata tccattccgg
     8281 ccataatgct ttgatagaaa gcttctttca ggttttcggc agtgccatgc aagtcgtaca
     8341 tgtgttcaat gtccatccag tcgctcacga taaagccttt gaagccccat tcgtttcgca
     8401 ggacatcatt cattaaccat tcattggcat gacaaggaat tccgttcagt tcgttgtgag
     8461 ctgtcatgaa cgacatggca ccagcatctg ctccggcttt gaagggagga aagaaaacct
     8521 cgcgaatggt tcgctccgac aaatcggcag gtgcaccgtt cgtaccgttc agtggttcac
     8581 tgccgcctac gaaatgcttg acacaagcca gtacatcctc gttgctgttc agattgccct
     8641 gataaccctt cacagtttgt tcacccagac gagataccag atatggatcc tcgccatagg
     8701 tttcacccac acggccccag cgctggtcac gggctacttc tacatttgga ttaaacgtcc
     8761 agtgcatatt cattgcgcgc atttctttgg ctgtctggcg ggcaattttg tatgccattt
     8821 cggtgtcgaa cgatgatgcc agaccgatat ttgttggata tactgtatta tcgggagcat
     8881 tggcattgcc atgaatggca tcaataccaa acaaaatggg tatctgcagc ttgctttgca
     8941 tggctaacga ttggagataa ttggcttccc tgagtgttag tacgtgaaga aacgaaccaa
     9001 ttaatccgtt ttttgtccat tcctcgacat ccttatgagt aaatccgggg taaaaggcac
     9061 tggcggtgtt ggttttcaat tcttcttcgc tcagattggc aacagcagat ttaatgtgtt
     9121 ctatacccac aaactgattc atttgaccaa ctttttcctc gagggtcatg cggctgagaa
     9181 gatcgtcaat tcttttctca atcggggcat tggggtcttt gtagagtggt tttttatcag
     9241 ctgctttcag gtttacggtc atggtgaata ataagcttat caaaataatt aatgttctca
     9301 tatatagcga tttaaaaatt gtcagttctg aaaggtacca caggtaatcc acgagtgttg
     9361 gccagatttc ctggttggaa attgcggaaa cagtagcgcg ctgcaatcgg attttgaatt
     9421 ttatcgctgg aaagaattat tttcagatta ttattgtcga gtttggcgga agcagggatg
     9481 aaaattttat cgcttccaca aatttcaaat cccctgatgt cattttctct gctgaaccca
     9541 tcctgtgcat aactgaaaga tagaattatt ttaccgtctc tgatttccat tgatttatat
     9601 tcaggtccgt gggcggcaat gcttttcaat tcgtaagttt ggttcagtgc aagataagcc
     9661 agccgtttcc ccacatctgt tttatttttc ggatgaatat ttattttttc atattcctcc
     9721 accaaatcat ttgtcgatat cattccactg ttcggaatga tggattgagc tttgaactga
     9781 gcttcacgca gcaatgcacc cgaaattcct gtttctccat cgccatatat ccagggtgca
     9841 atttcggcat aatagaaggg aagattattg tttttccagt cttttctcca aagttcaacc
     9901 attgtggcaa aacggcgggc gtaaacgtca tgtttaccca cgttcgattc tccctgatac
     9961 cagataaaac ctttcagtgt gaagtttgac agtggtttta gcataccatt atacatcagc
    10021 agtggtcgtt tccattcatg ttcggtttca ataccttttt gggtgaggtc aatatcggga
    10081 taggtttcca gaatttcgcg gtttgtccag ctttcgacac gtgaacctcc ccagctgcaa
    10141 acaatgattc ccaccggcac attcagtgta ttgtaaagca tttgagcaaa atgataagct
    10201 gtagcgctga aaatgggtgc attgtttgga ttgctgagca tccatttacc cgggcaggtt
    10261 tcctgaggtt cgtatgccgg agtttttggg acaacggcaa aacgaattcc tttgttttgc
    10321 ccggacaagg ctatggtttc gttggcgtcc tgtatgggac agttccaaaa tccgttgagt
    10381 ggcatttcca tattgctctg acctgatgca agccagactt caccgatcag aatatttttc
    10441 agcgtcagcg tttcaccatc cgaaaaagta agggtctgag gtgtaaatgt agcctccggt
    10501 gtttggattt tagccaacca atttccttta ttatccgttt tcacggtcgt ggtcattttc
    10561 atccatgaag gctgcacttt gacaatattg tttggactgg cttttcccca aatatttact
    10621 gttgtactct gctgtagcac catgttgtcg ctgagaattt cgggcagtgt aaccttggca
    10681 aatgccgcag aggttaaagc cgaaagaatg attaatacaa taaatttact tt
//
LOCUS       DAOPYB010000104        10372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_7472_length_10372_cov_10.251817, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000104 DAOPYB010000000
VERSION     DAOPYB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7472_length_10372_cov_10.251817"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..2021
                     /locus_tag="PKH58_08620"
     CDS             126..2021
                     /locus_tag="PKH58_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNX89131.1"
                     /translation="MTNYMQVENLTKSFGDNLLFENISFTITDNQRVALIAKNGTGKT
                     TLLNILADREGYDSGNITLKRDLRIGYLEQSPDFPKELSVIDACLQTDNDIVRTIADY
                     EHVMLHPDKDKMTDVLARMEILKAWDYETRIKQILGKLKITDFEQKIGHLSGGQLKRV
                     ALANVLISEPDLLILDEPTNHLDLEMVEWLEDFLKRSTMSLLMVTHDRYFLDRVCNTI
                     LEIDNQSLFAYSGNYSYYLEKRQERIDTFNAETESMRNIYRRELEWMRSTPQARSHKS
                     RGRIDRFKDIEERAKQRQTNENIKLGVKSSYLGSKIFEANYICKSFGDYKILDNFYYN
                     FARYEKMGIVGKNGTGKTTFLKMLLGHVKPDSGSFDIGDTVIFGYYSQDGLKFDEQMK
                     VIDVVQDIAEEIDLGGGKKLSASQFLQHFLFAPETQHNYVYKLSGGEKRRLYLCTVLM
                     KNPNFLVLDEPTNDLDIVTLNILEDYLQNFKGCVIVVSHDRYFMDKVVDHLLVFNGNA
                     EIKDFPGNYTQYRESGTAPQPPKGGVLEGNTSNNSKKTAVTNNPQSTTDGGNKPRKMS
                     FREKQEFEALEKEIPQLEAEKKDIENQLSSGTFDNDTIIKISSRFAELTDLIDEKTMR
                     WLELSEI"
     gene            complement(2226..3200)
                     /gene="argC"
                     /locus_tag="PKH58_08625"
     CDS             complement(2226..3200)
                     /gene="argC"
                     /locus_tag="PKH58_08625"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNX89132.1"
                     /translation="MKKIKAGIIGGAGYTAGELLRVLINHPDVEIAFVNSSSNAGNSV
                     VSIHGGLIGETDLIFTSELPLDVVDVLFLCSAHGDSKKFLDANAVPTGVKIIDLSTDY
                     RQARPEHDFVYGLPEVNKAAIQQANHIANPGCFATAIQLAILPLAAAGLLTDDVHINA
                     ITGSTGAGVKPSETSHFSWRNNNISVYKAFEHQHLQEIGQSVHQLQPGFDKNLNFIPV
                     RGNFARGIFASTYTRCPLSAEEAKALYKEFNKDAAFTFVADKNPDMKQVVNTNKAIVY
                     VEKHGDNILIISMIDNLLKGASGQAVQNMNLMFGLDEKAGLNLKSTGF"
     gene            complement(3497..4174)
                     /gene="trmD"
                     /locus_tag="PKH58_08630"
     CDS             complement(3497..4174)
                     /gene="trmD"
                     /locus_tag="PKH58_08630"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNX89133.1"
                     /translation="MRIDIISAVPELLESPFNYSIIKRAREKKLVEICVHNLRDYTED
                     KHRRVDDYAFGFGAGMVLQIEPVDNVISKLKAERKYDEIIYTSPDGQKFDQKLANRYS
                     MAENLIILCGHYKGIDHRIREHLITKEISIGDFVLTGGEMPAAMMTDAIVRLIPGAIG
                     DETSALSDSFQDNLLAPPVYTRPADYKGWKVPEILLSGHQARVEEWLHQQSMERTQRL
                     RPDLLNE"
     gene            4414..5709
                     /gene="gldE"
                     /locus_tag="PKH58_08635"
     CDS             4414..5709
                     /gene="gldE"
                     /locus_tag="PKH58_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HNX89134.1"
                     /translation="MLNNIIVNPFTLTSGILLFLGLIFLCFTAFFSGAEVAYFSLDQR
                     TIDDFDESEEKCDKAAVRLLGNPQKLLATILISNSLTNIAVIVLLVNFTATVFDFSFA
                     PGLGFLIQVAVIGFLILIFGEIMPKVFSSEYAKITVQRSSRILLNLQNFFNPFVELFA
                     NSRALVNSKLTSRNYNNISMEELTQAFDITAETPVEDKEILEGIIKFGNIQVADIMTS
                     RVDIVDVDIKMNYTQLLNLIIKSGYSRMPIYLGTRDNIKGILFSKDLLPHLDKPSTFR
                     WQTLIRPPYYVPETKKIDDLLNEFQENRVHLAIVVDEYGGTSGLVTLEDILEEIVGDI
                     SDEYDDEETLYTKIDNHTFLFEAKILLNDFFKIAEISKEDFVKKTEDVETLAGLILEL
                     KGEMPVKNERIGYGRYVFEITSVDNRRIKKVKLHIKSNP"
     gene            5720..6310
                     /gene="gldD"
                     /locus_tag="PKH58_08640"
     CDS             5720..6310
                     /gene="gldD"
                     /locus_tag="PKH58_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HNX89135.1"
                     /translation="MNRQKFYLLSIAVLLIYGLLTVGCGEATIPKPYGYFRVDLPAHR
                     YERLDSAPLPYSFEKSVYARVQPRMEEGENFWIDVKYPSLNASLYCSYKPVHSNLFEL
                     SEDTRKIVYKHTVRADGIGEKVFENPDKKVYGILYDLTGNTASSVQFILTDSVKHFFR
                     GALYFENVPNKDSIAPMSAYIREDVIRLMESFEWKK"
     gene            6324..6968
                     /locus_tag="PKH58_08645"
     CDS             6324..6968
                     /locus_tag="PKH58_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNX89136.1"
                     /translation="MIDYLVVSHQDACICIFENKFSSDDLLNKFVDSGIYSHELSLLK
                     AENRKKEFLGLRVALKHCLKGQEKQIGYTEKGKPVLADNSSKISFSHCKGWVAVITHP
                     VLEVGIDIENPSEKLNTLYKRFLCEEELQDYLKDENFDYLRIIWSAKEALFKIIGDEA
                     VNFARQLQILPFQVETEGDVTGIHRPTGKKFNIHYLLNDSYTLAWCIDQTNQHP"
     gene            6965..7702
                     /locus_tag="PKH58_08650"
     CDS             6965..7702
                     /locus_tag="PKH58_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNX89137.1"
                     /translation="MISIYKNILSNAGGNRKMLAVLLDPDKCTGSVLTDTLAEFDKYT
                     PDFIFIGGSTGMCNTEGLLLSLRNVPSPKILFPGDASQFCSTADGLLYLSLISGRNPD
                     YLIGQHVQSAIEIRKSDVEVIPTGYILIDGGNISAVERVSNTSPLNPDDVTTCLSTAI
                     AGELLGLKLIYLEAGSGAEYPVPETTIRAVKQVLNIPLIVGGGIKTEEQLDAAFHAGA
                     DLVVIGNLFETRTMRISELVNYVRNFR"
     gene            complement(7774..9390)
                     /locus_tag="PKH58_08655"
     CDS             complement(7774..9390)
                     /locus_tag="PKH58_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX89138.1"
                     /translation="MNKKNVFIIVAFVCALLVGLLLGNLIAFRASQGENIKSLTGLFS
                     GKSSKIDNLLSLINEQYVDTIDIDSITDEVMTDLVSKLDPHSVYIPTQDLEAVNSELE
                     GSFSGIGVQFNIQNDTVMIVSVISGGPSEKVGLMAGDRIVKVNDTIFAGKNVVSNEKV
                     MKKLRGPKGTKVKLSVKRHGTKELLSYSITRGDIPVNSVAVAYIIEPGVGFIGLNSNF
                     AKTTYDEFINAIAELRAKGAKKYIIDLRENSGGLMDVAIRMINEFLPKGEMIVYSEGK
                     AYPRYEAKADGSGTCINNEMVVLIDEFSASASEIFAGAIQDNDRGLIIGRRSFGKGLV
                     QQQIPFSDGSAVRLTVARYYTPSGRSIQKPYVKGDAKAYEEDIMNRYIHGEFDSKDSI
                     HVSGTLKYRTLKGRTVYGGGGIMPDIFVPRDTLKYTPYLTKVVNYGYIYQFAFQYSDK
                     YRAKLNTFKTWQDMDKYLTTENLLNQFTAFAASKGVASNNREINISKELITNQIKAYV
                     VRNILGDKGFYPVLYKNDETVKKALETLRK"
     gene            complement(9412..9837)
                     /locus_tag="PKH58_08660"
     CDS             complement(9412..9837)
                     /locus_tag="PKH58_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNX89139.1"
                     /translation="MDKQLLLDNRYMRMARIWAENSYCERRKVGALLVKNKMIISDGY
                     NGTPSGFENKCEDENNVSKAYVLHAEANAISKVARSHNSSDNATLYVTASPCMECSKL
                     IIQAGIKRVVYGEKYRIMDGVELLERAGIEVVYLDNVEK"
     gene            complement(9990..>10372)
                     /locus_tag="PKH58_08665"
     CDS             complement(9990..>10372)
                     /locus_tag="PKH58_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan synthetase"
                     /protein_id="HNX89140.1"
                     /translation="SKLKATVNAARKQYPDRKLVACMELHTFSSLTEEFLPQYAGCMK
                     DADVAFVYYNPEVIQHKRLKELVPYEVRSAFGGNNITVFTDTNELQKTLRSMNYDNSV
                     LLLMSSGNFSGINLIEFAEELLAP"
BASE COUNT         3031 a         2307 c         2125 g         2909 t
ORIGIN      
        1 taaataaaat aagtttaaaa cgggaacaca atttatgatc gttctcccgt ttttcatcta
       61 aacacatcat ttataattca taacttagcc cgaattctta tctttgcaaa aaataatatg
      121 accgtatgac caattatatg caggtagaaa atctgaccaa atcgtttggc gataatctgc
      181 tttttgaaaa tatatccttt accatcaccg acaatcaacg ggtggctctg attgccaaaa
      241 acggaaccgg aaaaacaaca ctcctcaaca ttcttgccga ccgagaaggt tacgacagcg
      301 gtaacatcac cttaaagcgc gatctgcgaa tcggttatct ggaacagtcg cccgattttc
      361 caaaagaact ttcagtaatc gacgcctgcc tgcaaacgga taatgacatt gtacgcacca
      421 tagctgacta tgaacatgtc atgctgcatc ccgacaagga taaaatgaca gatgtgctgg
      481 ctcgtatgga aattctgaaa gcctgggact atgaaacacg catcaaacaa attctgggca
      541 agctaaaaat tactgatttc gaacaaaaaa tcggtcattt gtcaggcgga caactcaaac
      601 gggtggctct ggccaatgta ctgatcagcg aaccggattt attaatactc gatgaaccaa
      661 ccaaccacct cgatctcgaa atggtggaat ggctggaaga ttttctgaaa cgatccacca
      721 tgtcgctgct catggttaca cacgaccggt attttctgga ccgtgtctgc aacaccatcc
      781 tcgaaataga taatcagtct cttttcgcct actccggcaa ttacagttac tatctggaaa
      841 aacgtcagga acggattgac acattcaatg cggaaaccga aagcatgcga aacatttacc
      901 gccgcgagct ggaatggatg cgaagcacgc ctcaggcacg atcacacaaa tcacggggcc
      961 gcatcgaccg attcaaagac attgaagaac gggctaaaca acgtcagacc aatgaaaaca
     1021 tcaaactcgg cgtaaaatca agctaccttg gctctaaaat attcgaagcc aactatatct
     1081 gcaaatcgtt cggcgactat aaaattctcg acaactttta ctacaacttt gcccgctatg
     1141 aaaaaatggg tattgtaggt aagaacggaa cgggtaaaac aactttcctg aaaatgttgc
     1201 ttggacatgt aaagcccgac agcggaagtt ttgacatcgg cgatacggtt attttcggat
     1261 attacagcca ggacggacta aagttcgatg aacaaatgaa ggtgattgat gtcgtacagg
     1321 atattgccga ggaaatagac cttggaggcg gcaaaaaact ttcggcatcg cagtttctgc
     1381 aacacttcct ttttgctccc gaaactcagc acaactacgt atacaaactc agtggcggtg
     1441 aaaaacgccg gctttacctc tgcaccgtgc tgatgaaaaa tccgaatttt ctggtactcg
     1501 atgagcccac caatgatctg gacatcgtta cattgaatat tctggaagat tatctgcaaa
     1561 acttcaaagg ctgtgtcatc gtggtcagtc acgaccgtta tttcatggac aaagtggttg
     1621 atcatttgct cgtattcaac ggaaacgcag agataaaaga ttttccggga aactatacgc
     1681 aatacagaga aagcggaaca gccccccaac cccctaaagg gggagttttg gaagggaata
     1741 cttctaataa ttctaaaaaa actgccgtca ccaataatcc tcaaagcaca acggacgggg
     1801 gaaataaacc ccgtaaaatg agtttcagag aaaaacagga atttgaagca ctggaaaagg
     1861 aaataccaca gctggaagct gaaaaaaagg acattgaaaa tcaactctca tcaggcacat
     1921 tcgataatga tactattatt aaaatatcgt cacgatttgc cgaactgaca gatttgattg
     1981 atgaaaaaac gatgcgctgg ctcgaattga gcgagatcta gatataaaac tgataagacc
     2041 tattaaaaac gcaattggta gccacgcctg actaccaatc tttgttaacc ttaaatctaa
     2101 tactatgaaa aaatcacaat gcaaatgtaa gtgtataaat tacttttata aaataattca
     2161 cccattttgt tgcatttttt ttcgcaaacc tttgctatca gtgttttttt cggaatgaag
     2221 ttatcttaga aacctgtgga tttcagattt agtccggctt tttcgtcgag accaaacatc
     2281 aggttcatat tctgaacagc ctgccccgat gctcctttca gcaaattgtc gatcatggaa
     2341 atgatcaata tattatcgcc gtgtttttcc acatacacaa tggctttatt cgtgttaacc
     2401 acctgcttca tatccggatt tttatcggca acgaatgtaa aagcggcatc tttattgaat
     2461 tctttgtaca gtgcttttgc ttcctcggca ctcaacggac atctggtata ggtggaggca
     2521 aaaatgccgc gtgcaaagtt accgcgcaca ggaatgaaat tcagattttt atcaaatccg
     2581 ggttgcagtt ggtgtaccga ttgtccgatt tcctgcaagt gctgatgttc aaaagctttg
     2641 taaacagaga tattattgtt tcgccagctg aagtgtgatg tttccgaagg tttcaccccg
     2701 gcacccgttg aacctgtgat ggcattgata tgcacatcgt cggtgagtaa accggcagct
     2761 gccagaggca gaatagccag ttgaatggca gtggcaaaac atcccggatt ggcaatatga
     2821 ttagcctgct gaatggcagc cttattcact tcgggcagtc catacacaaa gtcgtgttcg
     2881 ggacgtgcct ggcggtaatc agtggacaaa tcgatgattt tcacgccggt tggaactgca
     2941 ttcgcgtcga gaaacttctt gctgtcgcca tgtgcagagc acagaaaaag cacatcaaca
     3001 acatccaggg gcagttcact ggtaaaaatc agatccgttt cacctatcaa accaccgtga
     3061 atggatacta cggagtttcc tgcgttgctg ctgctgttta caaacgctat ttccacgtcg
     3121 ggatggttga tcaacacccg caataattct ccggcggtat atcccgcgcc accaatgata
     3181 ccagctttaa tttttttcat ccgaatttat tttttacaac cacacaggtt gggtttattt
     3241 attcaaatat ccaattaaaa aaatccatac tgccagcacg ataaacgtaa cgctgtagta
     3301 tgaaaattta cggtttttat gtcttatcaa atacatcagc gacaccactg caaaaactcc
     3361 gcccgtcagt tcgagcaaat gcagggttag ctcgggcacc cttcttcttg agttgatagc
     3421 cgctttttta tcataaataa atacagcgga acttatcaaa ctgacaatta taaaataaat
     3481 gtagcaaact tccatcctat tcgttcagca aatcgggccg caaacgttgc gtacgctcca
     3541 tcgactgctg atgcaaccat tcctccaccc tagcctgatg tccggacagc aggatttctg
     3601 gaactttcca tcccttgtaa tcagccggac gggtataaac cggtggagcc agcagattgt
     3661 cctgaaaact atccgacaat gctgaggttt catctccaat tgctcccgga atcagcctga
     3721 cgatggcatc ggtcatcatg gccgcaggca tttcgccacc cgtcagcaca aaatcaccga
     3781 tactgatttc cttcgttatc agatgttcgc ggatacggtg atcgatacct ttgtaatgtc
     3841 cgcaaagaat gatcagattt tcggccatcg aataccggtt agccagtttc tgatcaaatt
     3901 tctgaccgtc gggcgaggtg taaataattt cgtcatactt tcgttcggct ttcagtttcg
     3961 agatcacatt atcaacaggt tcaatctgca gcaccatgcc tgctccaaac ccaaaggcat
     4021 agtcatcgac gcggcggtgt ttatcttcgg tataatcgcg aagattatgc acgcagattt
     4081 ccaccagctt tttctcgcgt gctcttttga taatcgaata gttgaaaggg ctttcgagca
     4141 attctggaac ggcagatatg atatcaatac gcataacaaa aagatttttg caaaaataca
     4201 aaaaaaacac ggctctccct aaagcggaaa ataattctga caaaacagag gaagatatgg
     4261 aacttgttcc ttacagtgct ggtattttca gtcttccaat ctgattttta tcattttact
     4321 acaaataaaa tcaaatttat gatgtatatt tgcatttagt ttgtaatctc ccatgtaact
     4381 atttcggtgc acggcagcta atctattaaa ccaatgctca acaacatcat tgtcaatcca
     4441 tttaccctta cgagcggcat tctgctgttt ctcggactga tttttctgtg ttttacagca
     4501 ttcttttcgg gtgccgaagt agcctatttt tcactggatc aacgcacgat tgatgatttc
     4561 gacgaaagtg aagaaaaatg tgataaagct gctgtacgct tgcttggaaa tccgcagaaa
     4621 ctgctggcca ctatccttat ctccaacagt ctgaccaaca tcgcggtcat tgttttattg
     4681 gtaaatttca ctgctacggt gttcgatttt tcatttgctc cgggacttgg atttctcatt
     4741 caggtagctg taatcggttt tctgatactt attttcggag aaataatgcc caaggtattt
     4801 tcttcggaat atgccaaaat cactgtgcag cgcagctcac gcatcctgct gaacctgcag
     4861 aattttttca atcctttcgt ggaattgttc gccaattcca gagctttggt caacagtaaa
     4921 ctgacaagcc gcaattataa caacatttcg atggaggaac tgacgcaggc attcgacatt
     4981 accgctgaaa cgcccgttga ggacaaggaa attcttgaag gaattattaa attcggcaac
     5041 atacaggtag ccgacatcat gacatcacgg gtggatattg tagatgtgga tatcaagatg
     5101 aattacacgc agttactcaa tcttattatc aagtccggat attcaagaat gcctatttac
     5161 ctgggcacac gcgacaatat caagggaatt ctttttagca aagatttgct tccacatctc
     5221 gacaaaccgt cgacattccg ctggcaaacg ctgatacgac caccctacta cgtgcctgag
     5281 accaaaaaga ttgacgattt gctgaatgaa tttcaggaaa accgggtgca tcttgccatc
     5341 gtggtggatg aatatggcgg cacatcggga cttgtaacgc tcgaagacat tctggaagaa
     5401 attgtaggtg atatcagcga cgaatacgat gatgaagaaa ctctttacac caaaatagat
     5461 aatcatacgt tcctgtttga agcaaaaatt ctgttgaatg attttttcaa aatagctgaa
     5521 atatcgaaag aagattttgt aaaaaagacc gaggacgtgg aaacgctggc aggactgata
     5581 ctggaactga agggtgagat gccggtaaaa aacgaacgaa tcggatatgg acgctatgtg
     5641 tttgaaatca catcggtgga caacagacgc atcaaaaaag taaaacttca tatcaagagc
     5701 aatccctaaa aaaatcagga tgaatagaca gaaattttat ctcctttcaa tcgctgtcct
     5761 gttgatttac ggactgctta cggtgggatg tggtgaagct accattccaa aaccgtacgg
     5821 ttatttccgg gttgatttac cggctcaccg ttacgaacgg ctggattcgg ctccgcttcc
     5881 ctactctttc gaaaagtcgg tctatgccag agttcagccc cgaatggaag aaggagaaaa
     5941 tttctggatt gacgtgaaat atccaagcct gaatgccagc ctctattgca gctacaaacc
     6001 ggttcattcc aacctttttg agctgagcga agatactcgc aaaatagtgt acaaacatac
     6061 tgtaagggca gacggcattg gtgaaaaggt ttttgaaaat ccggacaaaa aggtgtacgg
     6121 aattctttat gacttaaccg gaaataccgc ctcatcagta cagtttattt taactgacag
     6181 tgtaaaacat tttttccgtg gcgctttata ttttgaaaat gttccaaaca aagattctat
     6241 tgctcccatg tcggcttata tccgcgaaga tgtcatacga ctgatggaaa gttttgaatg
     6301 gaaaaaataa ttctttttta cgaatgatcg attatctagt tgtttcacac caggatgcct
     6361 gtatttgtat tttcgaaaac aaattctctt cagacgattt gctgaataag tttgtcgatt
     6421 ccggaattta cagtcatgaa ctatccctgc ttaaagccga aaacaggaag aaggaatttc
     6481 tgggactccg agtggcattg aaacattgtc tgaaaggaca agaaaagcaa attggatata
     6541 ccgaaaaagg gaaacctgta ctggcagaca acagttctaa aataagcttc tcgcattgta
     6601 aaggctgggt agccgtgatc actcacccag ttcttgaagt tggaattgat atagaaaatc
     6661 cgagcgaaaa gctcaatacg ctgtataaaa gatttctgtg tgaagaggaa ttacaggatt
     6721 acctgaagga cgaaaacttc gattatctgc gtatcatctg gtcggcaaaa gaagcgctct
     6781 ttaaaatcat cggtgatgaa gctgtaaatt ttgcccgaca actgcagatt ctaccatttc
     6841 aggttgaaac ggaaggagat gtaacaggca tacacagacc taccggaaaa aagttcaaca
     6901 tacattatct gttaaacgac agttacactt tagcttggtg tatcgaccaa acaaaccagc
     6961 atccatgatc tctatttaca aaaatatcct gtcaaatgcc ggaggcaaca ggaagatgct
     7021 tgctgtactg ctcgatcccg ataagtgcac cggttctgta ttgactgaca ctctggctga
     7081 atttgataaa tacacacccg acttcatttt tattggtgga agtaccggca tgtgcaacac
     7141 cgaaggattg ctcctgtcgc tcagaaatgt gccgtcaccc aaaatactct ttccgggcga
     7201 tgcctctcag ttctgctcca ctgccgatgg attgctttat ctttccctta tttccggacg
     7261 aaatcctgat tatctgatcg ggcagcatgt gcaatcggct attgaaatca ggaaatctga
     7321 cgtggaagtc attcccaccg gctacatcct gattgacggg ggaaatatat cggctgtaga
     7381 gcgggtcagc aacaccagcc cgctaaaccc tgacgatgtt acaacctgct tgtcgacagc
     7441 cattgccggt gaactccttg gtctgaaatt gatttatctc gaagccggaa gcggagctga
     7501 atatcccgtt ccagaaacta caatccgtgc tgtgaaacag gtactgaata tcccgctgat
     7561 tgtgggtgga gggattaaga cggaagaaca actcgatgcc gctttccatg ccggcgctga
     7621 tctggtagtg attgggaacc tgtttgaaac ccggacaatg agaatttccg aacttgtaaa
     7681 ctacgtaaga aatttccgat aaaacaaatt aatacagaaa caaaaaatgc ctgaacgaca
     7741 atgtctttca ggcatttata tattaacggt tttttatttt ctcagagttt ccagtgcttt
     7801 tttcactgtt tcgtcatttt tataaagtac aggataaaag cctttatctc caagaatatt
     7861 tctcaccacg tatgctttta tctgattggt tattaattcc tttgagatat taatttcgcg
     7921 gttgttggat gcaactccct tcgaagctgc aaaggctgta aattggttaa gcaggttctc
     7981 ggttgtcaga tacttgtcca tatcctgcca ggttttaaag gtgttcagtt tagctctgta
     8041 tttgtcggaa tactgaaacg caaactgata gatgtagccg taattcacca ctttcgtaag
     8101 gtaaggtgta tattttaaag tatcgcgggg cacgaaaata tcgggcataa tgcctccgcc
     8161 gccatacact gtccggcctt tcagggttct gtatttcaat gtgcctgaca catggatgct
     8221 gtctttgctg tcgaattcac catgaatata ccggttcatt atatcttctt cgtaagcttt
     8281 ggcatcacct ttcacgtaag gtttctgaat gcttcttccc gaaggggtat aatatcgtgc
     8341 aactgtgagg cggaccgcgg aaccatcgga gaagggaatt tgctgttgta caagcccctt
     8401 accgaacgac cggcgaccga taatcagtcc gcggtcatta tcctgaatag ctcctgcgaa
     8461 aatctcgctg gcggaagcac tgaattcgtc aatcagtacc accatttcat tattgatgca
     8521 ggttccggaa ccgtcggctt ttgcttcgta gcgcgggtag gcttttcctt cggaatatac
     8581 aatcatctct cctttcggca gaaactcgtt aatcattctg atagccacat ccatcagtcc
     8641 tccgctgttt tcgcgcaggt caatgatgta tttcttcgct cctttagccc ggagttctgc
     8701 aatggcattg ataaattcgt cgtaggtcgt tttggcaaag ttgctgttga gaccgatgaa
     8761 gcccactcct ggttcaataa tataagctac tgccaccgaa ttgaccggaa tatcgccacg
     8821 tgtgatgctg taggacaata attcttttgt tccgtgacgt ttcacactca attttacttt
     8881 cgttcctttt ggtccgcgga gtttcttcat cactttttca ttgctcacca catttttacc
     8941 tgcgaaaatg gtatcgttaa ccttcacaat acggtctccg gccataagtc ccaccttttc
     9001 ggaaggtccg ccactgataa ccgacacaat catcactgta tcattctgaa tattgaattg
     9061 tacgccaata ccactaaaac tgccttcaag ttcactgttg acggcttcca ggtcttgtgt
     9121 gggaatatat accgagtgcg gatcaagttt tgataccaga tcggtcatca cttcatcggt
     9181 aatagagtct atatcaattg tatcgacata ctgttcattg attagtgaaa gtaaattatc
     9241 tatcttgctg ctctttcccg aaaataatcc tgtcaggctc ttgatatttt ctccctgact
     9301 ggctctgaaa gcgatcagat tacccaataa cagtccgaca agtaatgcac atacaaatgc
     9361 aactatgatg aaaacatttt ttttattcat atatgtaaaa gttcaatatg tttatttttc
     9421 aacgttgtcc agataaacca cttcaatgcc ggcacgttcc agcaattcca caccatccat
     9481 gatacggtat ttttcaccgt aaaccacccg ttttattcct gcctgaatga ttagttttga
     9541 acattccata cagggcgaag ccgttacata taacgtggca ttatcgctgc tgttgtgcga
     9601 acgggcaact tttgagatgg cattcgcttc ggcatgaagc acataagcct tcgacacgtt
     9661 gttttcatct tcacatttat tctcaaagcc cgaaggtgtg ccattgtagc catctgaaat
     9721 aatcatcttg ttcttcacca gcaacgcacc caccttgcgg cgttcgcagt aggagttttc
     9781 ggcccagatg cgggccatgc gcatataacg attgtctaat aataattgtt tgtccatata
     9841 aaaatccaag ccccctaaat ccccctcaag ggggacttaa aaagaaaata aatagtttta
     9901 taatgataaa tgaaatatca ctaattgcat gaatttcact tttatgttta accatcactg
     9961 atgacccgac atattccccc ctgaaggggt tagggggcta acagttcttc tgcaaattcg
    10021 attaaattga tacccgagaa atttcctgac gacatcagca ggagaacaga attatcgtag
    10081 ttcatcgaac gcagcgtttt ctgaagttca ttggtatcgg taaaaacagt gatattattg
    10141 cctccaaatg ctgaacgaac ctcataaggc accaattctt tcaggcgttt gtgctgaata
    10201 acttcaggat tgtagtacac aaaagccaca tcggcatctt tcatgcatcc tgcatactgt
    10261 ggcaaaaatt cttccgtcag gctgctgaat gtgtgcagtt ccatgcatgc caccagtttt
    10321 cggtcgggat actgcttacg ggcagcattc accgttgcct tgagtttgga ag
//
LOCUS       DAOPYB010000105        10258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_7606_length_10258_cov_9.880721, whole genome shotgun sequence.
ACCESSION   DAOPYB010000105 DAOPYB010000000
VERSION     DAOPYB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7606_length_10258_cov_9.880721"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKH58_08670"
     CDS             complement(<1..565)
                     /locus_tag="PKH58_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX89141.1"
                     /translation="MNLYRGCTHGCIYCDSRSKCYQINHAFEDVEVKSNAPELLIQAL
                     KRKRKRCMIGTGSMSDSYLPLENQLLLTRRCLEIIEENGFGLSILTKSNRILRDLDLL
                     IKINQKAKCVVQMTITTFDENLCKKIEPDVCTTRKRFETLKIIHENNIPTIVWLCPIL
                     PFINDTEENLTGILNYCIEAGVKGILCF"
     gene            complement(635..1165)
                     /locus_tag="PKH58_08675"
     CDS             complement(635..1165)
                     /locus_tag="PKH58_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNX89142.1"
                     /translation="MGIEIYLQLFISFFKIGLFGFGGGYAIISLIQHEIEIHGWMTNS
                     EFTDIIAISQMTPGPIGINSATYVGYTASGTILGSAIATFAIVLPSFIIMLTLSKFFF
                     VLQGNKYMEYALLGLRPSVIGLIAAAALVLMNTENFIDYKSIILFAVAFLASFKFKLH
                     PILLIILSGIAGIILY"
     gene            complement(1165..>1389)
                     /locus_tag="PKH58_08680"
     CDS             complement(1165..>1389)
                     /locus_tag="PKH58_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNX89143.1"
                     /translation="IFKGIRPVVVALIAAPLYNMAKSAGVNWKTLFIPVVAALLIWGL
                     NISPVWVVVAAIAGGIIYGTLLTKKINKSN"
     assembly_gap    1390..1489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1490..1927)
                     /locus_tag="PKH58_08685"
     CDS             complement(<1490..1927)
                     /locus_tag="PKH58_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HNX89144.1"
                     /translation="MKDKLTAYIKKTATEIGFDAVGVARADYLAEDAAFYKSWLESGC
                     QAEMTYLERNQEKRLNPGLLVNGCKSVIVVLMNYFSESEQPSQAPKIAKYAFGSVDYH
                     AIIKEKLLLLENGIIENFGADCFNPAQQHRFVDSAPVMERRWAQ"
     gene            complement(2000..3130)
                     /locus_tag="PKH58_08690"
     CDS             complement(2000..3130)
                     /locus_tag="PKH58_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX89145.1"
                     /translation="MMRKLLFFSLVILFTACNNSGKFYVTGNIADAEKKMLYFEKTGL
                     VKDSLIDSVKLGANGYFRFKSALEEYPELYKLKLEGQQLVLAVDSTTGKIEIKGKASD
                     LITSEINGSVQSVEIQDLRKSVIKLQQEADGLNASQNSADSKAVLDTFIQHVTSHKKK
                     VIDLIMKNPRSMSSYFALYQQVSGTYLFSPFDKEDRPFYSAVATAFTTYMPKFDRSKN
                     LYNLVIAAIQNEREGRLQKAWQELQNTSAAGFIDIELNDAKGVARKLSSLTGKAVIID
                     FSAFETENNVAYTFELRDIYNKYSSQGLEIYQVSLDRNAMLWKKSVANLPWICVRDAD
                     GSVANMYNVQSIPTLYLINKQGTIVGRYGDVPQLAADISKAL"
     gene            complement(3143..3691)
                     /locus_tag="PKH58_08695"
     CDS             complement(3143..3691)
                     /locus_tag="PKH58_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNX89146.1"
                     /translation="MKKVFIFLADGFEELEAIAPIDILRRADIPVVSVSLTDSKIVIG
                     AHRIQLIADQLFSETDFSDNAFLVLPGGGEGTRNLAAHKQLNELLLHQFERNKNLAAI
                     CAAPSVFGNLGILSGKEATCYPGFEEKLVGAEISDQTIVKSENIITAKGPGVAIQFAL
                     KIVETLKSSELAHEIAKQLIFE"
     gene            complement(3713..4498)
                     /locus_tag="PKH58_08700"
     CDS             complement(3713..4498)
                     /locus_tag="PKH58_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX89147.1"
                     /translation="MEKSNIYGIIGSAIFCLLTFLILWFVVMPAPVIPEEDEGIMVSF
                     GDSPDGAGLGDGGVIKTDIDNVQPAPALSVPKPAVASRPQELLTQDNENTIAIATQKK
                     KEREKQRELEIQKLEQQRQVAAAAAEQKRQQEAIAKANDLMGGSFGKGGGSGSGTTAG
                     DTRQGNPAGSGTSGGHGWSLSGRSLVGSLATPSYPSNVEGKVTVAIRVDANGHVTNAS
                     VGSPTTISDAQTRNAAVSAARNTRFSGGSGVSVGTITYNFKLK"
     gene            complement(4504..4929)
                     /locus_tag="PKH58_08705"
     CDS             complement(4504..4929)
                     /locus_tag="PKH58_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX89148.1"
                     /translation="MSLKRKYRTDPSFSMSSMTDIIFLLLLFFVISSTMTTPNDIKLN
                     LPSSKSEKATKKVIAKVSIDREGNYFVSRNNEKSVPVTPESLEPYILNLVAQDTTTYL
                     ALYADEEISYKQVVRVLDIANQNKLKLVIATKAVANTEE"
     gene            complement(4995..5696)
                     /locus_tag="PKH58_08710"
     CDS             complement(4995..5696)
                     /locus_tag="PKH58_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX89149.1"
                     /translation="MNLLVILQAVNAAVAELALTQAAPEKMNLWTMAVYGGPIMIVLA
                     VMLALGVYIFIERLVTINKASQEDKSFMNRIKDYIHDGKLESAQKLCRDADTPAARMV
                     EKGISRLGRPMSDVLVAIENVGNLEIAKLEKSMGIMSTVAGGAPMLGFLGTVTGMVQT
                     FYTMTQNPSGVVQLRDLSTGMYQAMVTTVGGLIVGILAYFAYNYLISKIDSVMRKLES
                     NTLEFLDLLNEPVKK"
     gene            complement(5772..6485)
                     /locus_tag="PKH58_08715"
     CDS             complement(5772..6485)
                     /locus_tag="PKH58_08715"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNX89150.1"
                     /translation="MTTLSVNINKIATLRNARGGNVPDVCNVALDCEKFGAEGITVHP
                     RPDERHIRYQDVLDLKSVLTTEFNIEGNPIPEFIKLVKTVKPAQVTLVPDAHNAITSD
                     AGWDTKAQLKFLKDVVADFQQAGIRVSIFVDTNPVNIEYAAKTGTDRIELYTEPYASC
                     YFQNKAAAVAPFVEAAKLAKNLGLGINAGHDLNLDNLNYLHQNIPWLEEVSIGHALVA
                     DALYLGLEETIKRYKTALK"
     gene            6741..7634
                     /locus_tag="PKH58_08720"
     CDS             6741..7634
                     /locus_tag="PKH58_08720"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNX89151.1"
                     /translation="MPEKECYRIAIFGNLHRTELGLQVSQIFSFFQDKNIVVLLDEYM
                     QNYIHDTLHISFNNEELISGDNFTADLALSIGGDGTFLNTAARIGNKNIPILGINTGR
                     LGFLADIAKNEVIEALQGIFDNKYTIEKRSLMKVETLDGTRLDAPYALNDVALSKQDS
                     SSMIIVHAKTKGELINSYQADGLIISTPTGSTAYSMSVGGPIVVPQARSFVISPVASH
                     SLNVRPLIVPDDWVIELEVVSRSNSYQISLDGRSKVMDQSTRLRIRKADYEIKVLKPL
                     NHTFFETLRNKLLWGVDKRND"
     gene            complement(7631..8251)
                     /locus_tag="PKH58_08725"
     CDS             complement(7631..8251)
                     /locus_tag="PKH58_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89152.1"
                     /translation="MKIKTYLTSLILLFMLAACDTESPVKPYVYENEPEFTWGYGAFY
                     GKYYSNYNQNNNVLSLNLFTSGLTLNENNELTGTGQYLILEDVFVSPQDSMLAAGTYT
                     LAESGEPFTFFSGSKFEDNNEEVPSGAYMYYVEADESKSKIVYIKEGTFTVSRQGAEN
                     YSIDIQFRTNEKTEIKSHFNVPLDFYDLSAEPPAANVKRFLKIKKR"
     gene            complement(8314..9534)
                     /locus_tag="PKH58_08730"
     CDS             complement(8314..9534)
                     /locus_tag="PKH58_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX89153.1"
                     /translation="MKFPVWVAKRYLFSKKSHNTINVISGISAAGVAVGTLALVCVMS
                     VFNGFETLIESMFSAFDPDLRVSLVEGKTFDVSSPEFQQIRKMKSIVNFTEVIEENAL
                     LRFRDKQMPATIKGVSNDFRAMTRIDSIMFDGEFVLTDSLINKAVAGVGVATELGMNS
                     TLFDPLVIYAPKRTERINILRPENSFNSTDAYVSGIFIVNQAEYDNKYVFLSVETARG
                     LFGYDKNKVSKIELKINPSENQLKVQNEIKNILGKKFKVQNRYEQQESFFKIMKIEKW
                     ITYLILCFILLIAVFNIIGSLSMLIIDKRDDIQTLRNLGADNLLIKRIFVFEGWFISA
                     VGAVAGIILGTAICLLQEHLGLLKMGEGYVVDAYPVATHFFDILLVLATVLIMGLFAS
                     LYPVKYINSLKEPD"
     gene            complement(9577..9927)
                     /gene="rbfA"
                     /locus_tag="PKH58_08735"
     CDS             complement(9577..9927)
                     /gene="rbfA"
                     /locus_tag="PKH58_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNX89154.1"
                     /translation="METTRQQKIARLLQKELGEIFLLYAKKMQGTLITVTGVRISPDL
                     SVARAHLSIFPADKVKSVLEQVNADTKSIRFDLGKRVRHQLRIIPELYFHIDDSLDYL
                     ENIDNLLKQDKKEE"
     gene            complement(9939..>10258)
                     /locus_tag="PKH58_08740"
     CDS             complement(9939..>10258)
                     /locus_tag="PKH58_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNX89155.1"
                     /translation="VISHLFEALKAINLVHDGKETISAADLDELKSVFGLFIEDVLGL
                     KSQNEATESTEAYHKAIDLLLNIRLEAKKNKDWATSDKIRNELTALGFEIKDTKDGFE
                     WKI"
BASE COUNT         2870 a         2222 c         1953 g         3113 t
ORIGIN      
        1 caaaacaaag tattcccttc actccggctt caatacaata attcagaata cctgtcagat
       61 tttcttctgt atcatttata aatggtaata tcgggcaaag ccaaactatg gttggaatat
      121 tgttttcatg tattattttc agtgtttcaa acctctttcg ggtagtacag acatctggtt
      181 ctattttttt gcaaaggttt tcatcgaagg tggtaatcgt catctgcacc acgcattttg
      241 ctttctgatt aatttttatc agcaagtcca ggtcgcggag tatccggttc gattttgtaa
      301 gtatgctcag tccaaatcca ttttcttcta ttatttcgag acaacgacgg gttagcagga
      361 gctgattttc caacggcaga tacgaatcgc tcatggaacc tgtcccgatc atgcaccttt
      421 tccgttttct tttcaaagcc tgaatgagca gttccggagc atttgattta acttccacat
      481 cttcgaatgc atggtttatc tgatagcatt tgctgcggga atcgcaataa atgcaaccgt
      541 gagtgcatcc acggtataaa ttcattccat tttgggacga taatatgctt ttagcctcct
      601 taaagtgcat acatttttat ctctccggca gacgtcagta taaaattata cctgctattc
      661 ctgacagaat tattaagaga atggggtgca atttgaattt gaatgaagcc agaaaggcaa
      721 ccgcaaagag gataatactt ttataatcga tgaagttttc ggtattcatc agtacaagtg
      781 cagcagctgc tatcaatcca atgactgacg gccggagacc caataaggca tattccatat
      841 atttattccc ttgcagtaca aagaaaaatt tagataaggt caacatgatg ataaaggaag
      901 gtagcacaat ggcaaaagtc gcaatagctg aacccagaat ggtacctgaa gctgtataac
      961 ccacatacgt ggcgctatta ataccgatag gtccgggcgt catctgcgaa atggcaataa
     1021 tatcggtaaa ttcgctgttg gtcatccagc catgtatttc tatttcgtgt tgaataagcg
     1081 atatgatggc ataacctcct ccgaaaccga ataatccgat tttgaaaaag gaaataaata
     1141 gctgtaagta tatttcaatc cccattaatt cgatttatta attttctttg taagcaatgt
     1201 accgtaaatt attccacctg ctatagctgc aacaaccacc cagacaggtg aaatattcag
     1261 accccagatt aataatgcgg caacaacggg aataaaaagg gttttccagt ttacaccagc
     1321 cgatttggcc atattgtaaa gcggagcagc aatcagtgcc accaccacgg gacggatacc
     1381 tttgaaaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tgtgcccagc
     1501 gtcgctccat tacgggagcc gaatctacaa aacggtgttg ttgtgccgga ttaaaacaat
     1561 cggcgccgaa attttctata attccattct caagaagtaa tagtttctcc ttgatgatgg
     1621 cgtgataatc aacgctccca aatgcatatt ttgcaatttt aggagcctgc gagggttgtt
     1681 ctgattcgga aaaataattc atcagtacaa caatgaccga cttgcatcca tttaccagca
     1741 gcccggggtt aagccttttt tcctgattgc gttcgaggta agtcatttcg gcctgacagc
     1801 cactttccag ccaggatttg taaaaagctg catcttcagc cagataatca gcccgggcaa
     1861 cgccaacagc atcaaatccg atttctgttg ctgttttttt aatgtaagct gtgagcttgt
     1921 ctttcaattt ctcttacata tttaaaataa aaaaaacacg atagctgagt tttcatacat
     1981 atcgtgttgt taaatagtat caaagagcct tactgatatc ggctgcaagt tgaggaacat
     2041 ctccgtaacg tccaacgatg gttccctgct tgttgatcag ataaagcgtg ggtatcgact
     2101 gtacattata catattggcg acactgccat cggcatctct tacacaaatc cagggcaggt
     2161 tagcaaccga ttttttccaa agcatagcgt tgcggtccaa agaaacctga tagatttcga
     2221 gtccctgcga agagtattta ttgtaaatat ctctcagttc aaaggtgtat gccacattat
     2281 tttctgtttc aaaggctgaa aaatcaatga taaccgcttt accggtaagt gatgacagtt
     2341 tacgggcaac accttttgca tcgttcagtt cgatatcgat aaagccggca gcggaagtat
     2401 tttgaagttc ctgccaggct ttctgtagtc gtccttctct ttcgttctga atggctgcaa
     2461 taacgagatt atacagattt ttcgaacggt cgaatttagg catataggtc gtaaatgcag
     2521 ttgcaactgc tgaatagaac ggacggtctt ccttatcaaa cggcgagaag agataggttc
     2581 cgctcacttg ctgataaaga gcaaaatagg acgacatgga gcgcggattc ttcatgataa
     2641 ggtcaattac tttcttctta tgactggtca cgtgttgaat aaaggtatcc agcactgctt
     2701 tggaatccgc tgaattttgg gaagcattaa gtccgtcggc ttcctgttgc agtttaatga
     2761 ccgacttgcg gaggtcctga atttcgaccg actgaacaga accattgatt tcagatgtta
     2821 tcaggtccga tgcttttcct ttgatttcaa ttttccctgt tgtagagtca actgccagaa
     2881 caagttgttg cccttccagt ttcagtttat acagttcggg atattcttca agggcgcttt
     2941 taaatcggaa atatccattg gctccaagtt ttaccgaatc tatcagactg tctttaacaa
     3001 gtcccgtctt ttcaaaataa agcattttct tttcggcatc tgctatgttt ccggttacat
     3061 aaaacttacc ggaattatta caagccgtga ataagattac aagcgaaaaa aatagtaatt
     3121 ttctcatcat tcagtttatt ttttactcaa aaattaattg tttagcaatt tcatgtgcca
     3181 gttcggagct cttcagtgtt tctacaattt ttaatgcaaa ctgaatggca actcccggac
     3241 cttttgctgt gataatattc tccgatttaa cgatagtttg atcggaaatc tctgctccaa
     3301 cgagtttttc ctcgaagccc ggataacagg ttgcttcctt accggaaaga atacccaggt
     3361 ttccaaacac cgacggtgct gcacaaatgg cagccagatt tttatttctt tcaaactgat
     3421 gaagcaacag ttcattcagt tgcttatgag cagccagatt tcttgttcct tcaccaccac
     3481 cgggcaatac taaaaatgca ttatccgaga aatcggtttc agaaaaaagc tggtcggcaa
     3541 tcaattgtat ccgatgtgcg cctatgacta ttttactgtc tgtcaatgag acagatacaa
     3601 ccggaatatc tgcacgacga agtatatcga ttggtgcaat agcttccagt tcttcaaaac
     3661 catcggcaag aaaaataaat acctttttca tattcctgta ttttaattgt gactatttca
     3721 atttgaaatt atatgttata gtacccacag acactccact accgcccgaa aaacgtgtat
     3781 tgcgagctgc tgaaaccgcc gcgttgcggg tctgcgcatc agaaattgtt gtaggtgagc
     3841 caacgcttgc attggtcaca tgcccgttgg catccacgcg tatggctact gtcaccttac
     3901 cttccacatt gctcgggtaa gaaggtgtgg caagcgagcc aaccaacgac cgcccgctga
     3961 gtgaccatcc gtgtccaccg gatgtaccgc ttcctgcagg atttccctga cgggtatccc
     4021 ctgctgtcgt tccactacct gagcctcctc ctttaccaaa agaccctccc atcaaatcat
     4081 tagctttagc tatagcttcc tgctgtcgtt tttgttcggc agctgctgca gcaacctgcc
     4141 tttgctgttc taatttctgt atttccagtt cacgctgttt ttcccgctct tttttcttct
     4201 gggttgctat tgcaattgta ttttcattgt cctgagtgag taattcctga ggacgtgaag
     4261 ccactgccgg ttttggaact gataatgccg gagctggttg aacattatcg atgtccgttt
     4321 ttattacacc tccgtcaccc agccctgcac catccgggct atcgccaaaa ctcaccatga
     4381 taccttcatc ttcttcagga atcacagggg caggcatgac tacaaaccac aggatcagaa
     4441 atgtcagcaa gcagaaaata gctgaaccta taataccgta tatatttgat ttctccaaaa
     4501 ttgttattct tcagtatttg caactgcctt tgtagcaatc accagtttca gtttattctg
     4561 attagcaata tcgaggaccc gaactacttg cttatacgat atttcttcgt cggcataaag
     4621 agcaagataa gtggtagtat cctgagccac cagattaagt atatagggtt caagtgattc
     4681 gggggttacc ggcacggatt tttcgttgtt tcgtgaaaca aaataattcc cctcgcgatc
     4741 tatggatacc ttggcaataa ctttttttgt tgctttttca gacttactcg aaggcaaatt
     4801 cagtttgata tcattcggag tagtcatcgt ggacgatata acaaagaata aaagcaacag
     4861 aaaaattatg tcggtcatcg acgacatcga gaaagaagga tctgtcctgt attttctttt
     4921 taatgacatt ttataatcag tttattcatt catttagtaa tcattgaatt gtaaaggaat
     4981 aatttcattt tgaattattt ctttacaggt tcattgagta agtcgaggaa ttcgagggtg
     5041 tttgattcca gtttgcgcat tactgagtca atcttggata tcagatagtt gtatgcaaaa
     5101 taagccaaaa ttcccacaat caagccacca accgtagtaa ccatagcctg atacatacct
     5161 gtcgacaagt cgcgcagttg aaccacaccc gaaggatttt gtgtcattgt gtaaaaagtc
     5221 tgaaccatcc cggtaaccgt gccaaggaag ccaagcatgg gagcaccacc tgcaacggtt
     5281 gacatgatac ccatactttt ttccaatttc gcaatttcga ggtttccaac attctcaata
     5341 gccacaagca catcgctcat cggacgaccc aaccggctaa tccccttctc aaccatgcgt
     5401 gcagcaggtg tatcggcatc gcggcaaagc ttctgagccg attccaactt tccatcatga
     5461 atataatcct taatccgatt cataaatgac ttatcttcct gcgatgcttt attgatagtt
     5521 acaagtcgtt cgataaaaat atacactcca agtgcgagca tcacagccaa tacaatcatg
     5581 atgggtcctc catacacagc cattgtccac aaattcattt tttccggagc tgcctgtgtc
     5641 agagcaagtt ctgctactgc agcatttact gcctgtaaaa ttacgagtag gttcattttc
     5701 ttaaatttct aattttctga tttattgctt gttggtaaat atgttccagt ttcggaaata
     5761 taactacagt tttattttaa cgctgtttta taccttttta ttgtttcttc cagtcctaaa
     5821 tacaatgcat cagcaacgag tgcatgtccg atggaaactt cttcgagcca ggggatattc
     5881 tgatgcagat aattgaggtt gtccagattc agatcatgtc cggcattgat acccaaccca
     5941 agatttttag ctagtttggc agcttctaca aagggggcta cagctgctgc cttattctgg
     6001 aaatagcagg aagcataagg ttccgtataa agttcaatac gatcagtgcc tgtttttgcc
     6061 gcatattcta tgtttaccgg attggtatcc acaaaaattg aaacacgaat tcctgcctgc
     6121 tgaaaatcgg caacaacatc ctttaagaat ttcaactgag ccttagtatc ccaaccggca
     6181 tcggaggtga ttgcattgtg agcatctggc accagagtta cctgtgccgg tttcacggtt
     6241 tttacaagtt tgatgaattc aggtatcgga tttccttcaa tgttgaattc ggtagttagt
     6301 acggatttca aatccagaac atcctgataa cgtatatgtc tttcatccgg gcgtggatgt
     6361 acagtaatac cttcggctcc gaacttttca caatcaaggg caacattaca tacatcgggg
     6421 acattgcctc cgcgtgcatt ccgcaaggtt gcaatcttat ttatatttac acttaatgta
     6481 gtcatatatc gcagaattag aagggtaaga aaaaaatagt ttactcattt tccggtgagt
     6541 atctgcttat tttatttcat cattcagatt tcttaaactc aaattttatt tgttaatatc
     6601 aaatccattt cgtttctttg tatgtttgaa tgaattcata tttgcaaaaa taactcaaaa
     6661 aaacgagtat tcgattaaag aatttattct ttcgtatttt ttaatgattt tgaaaataac
     6721 acataaccct gtcagacttt atgccagaaa aagaatgtta tagaatagcc attttcggaa
     6781 atcttcaccg gacagaactc ggacttcagg tttcacagat attcagcttt tttcaagaca
     6841 aaaacattgt agtcttactg gatgagtaca tgcaaaatta tatacacgat actttacaca
     6901 tttcattcaa caacgaagag cttatttcgg gtgataattt tactgctgac ctggcgctga
     6961 gcattggtgg tgacggcact tttctgaaca ccgcggctcg tatcggcaat aaaaacattc
     7021 ctattctggg tattaacaca ggcaggcttg gttttctggc cgatattgcc aaaaatgaag
     7081 taattgaggc attgcaggga atttttgaca acaaatacac catcgaaaaa aggagcctga
     7141 tgaaagtgga gacactggat ggaacacgtt tagatgctcc ctatgcactt aatgatgtag
     7201 ctctttcgaa acaggacagt tcgtcgatga ttattgttca tgccaaaaca aaaggtgaac
     7261 tgattaacag ttatcaggct gatggcttaa ttatttcaac ccccaccggc tctaccgctt
     7321 attcgatgag tgtgggcggc cccattgttg tacctcaggc tagaagtttt gtaatttcgc
     7381 cggtggcgtc gcatagcctg aatgtaaggc cgctcattgt gcccgacgac tgggtgattg
     7441 aactggaagt tgtgagccgc agcaacagct atcagatttc tctcgacgga cgttcgaaag
     7501 tgatggatca aagcacaaga ctacgcatcc gaaaagcaga ctatgaaatc aaggtactta
     7561 aaccattgaa tcatacattt tttgaaacat taagaaataa attgctttgg ggtgttgata
     7621 aaagaaacga ctaacgcttt tttattttca gaaaccgctt aacgttcgcg gccggaggct
     7681 cagctgacag atcatagaaa tccagcggga cattaaaatg tgactttatt tctgtttttt
     7741 cattcgtcct gaactgaata tctatactgt aattttcagc accctgcctg cttacggtaa
     7801 atgtaccttc tttgatatac acaattttag acttggattc gtcggcttcg acgtaataca
     7861 tataagctcc tgaaggcact tcctcgttgt tgtcttcaaa tttcgatcca ctgaaaaatg
     7921 taaaaggctc accactttcg gcaagtgtat aagttcctgc agcaagcata ctatcctgcg
     7981 gcgatacaaa cacatcttcc aaaattagat actgaccggt acctgtcagt tcgttgtttt
     8041 cattgagcgt caaaccactg gtaaacagat ttaaagacag cacattatta ttctggttat
     8101 aattgctgta atatttgccg taaaacgcac catatcccca cgtaaattca ggttcattct
     8161 cataaacata aggtttcaca ggactttcgg tatcacaggc agccagcatg aacagcaata
     8221 taaggcttgt caggtatgtt ttgattttca tttatcgacc ggtattaaaa aatttctctt
     8281 tatcaaaata atgatttccg acagatataa agcctaatca ggctccttca gggaattgat
     8341 gtatttcacc ggatatagtg acgcaaataa gcccattatc agtaccgtgg caaggaccag
     8401 taaaatgtcg aagaaatgag ttgccaccgg ataagcatcg accacgtaac cttcgcccat
     8461 tttcaacaag cccagatgct cctgtaacaa acaaatagcc gtgccaagta taatccctgc
     8521 cactgctccc acagccgata taaaccaccc ttcaaaaaca aagatccgtt tgataagcag
     8581 gttgtcagct ccgagattac gcagtgtctg aatatcatcc cttttatcga taatcagcat
     8641 ggacagcgaa ccgatgatat tgaatactgc tatcaaaagg ataaaacaaa gtatgaggta
     8701 ggtaatccac ttctctattt tcatgatttt gaagaaactt tcctgctgtt cgtagcgatt
     8761 ttgaacctta aactttttcc caagaatatt tttaatttca ttctgcactt tcagctggtt
     8821 ctctgaagga ttgatcttca gctcaatttt agaaacttta ttcttgtcgt aaccgaacaa
     8881 gccgcgtgcc gtttctacag acaaaaatac atatttgtta tcgtattcgg cctggtttac
     8941 aataaaaatg cctgaaacat aagcatcggt agaattgaaa ctgttctcgg gacgaaggat
     9001 gttgatacgt tccgttcgtt tgggcgcata gattaccaag gggtcaaaca gggtagaatt
     9061 cattcccagt tctgtcgcca cacccactcc cgcaactgcc ttattgataa ggctgtctgt
     9121 gagcacaaac tcaccatcaa acatgatgct gtcaatccgg gtcattgccc tgaaatcgtt
     9181 gctgactcct ttgatagttg caggcatctg tttatcccga aaacgcagga gcgcattttc
     9241 ttcaatcact tcggtaaaat tgacaatgga tttcattttc cgaatctgct gaaattccgg
     9301 actgcttaca tcgaacgttt ttccttcaac cagcgaaact ctcaggtctg gatcaaacgc
     9361 cgagaacatg ctttctatca aagtttcaaa cccattgaaa accgacatca cacaaacgag
     9421 cgccagggtg cccacagcaa ctccggctgc cgaaattcct gatatcacgt tgatggtatt
     9481 gtgcgatttc tttgagaaaa gatatcgctt agccacccat accggaaatt tcaacgcccc
     9541 tgatttatgt gatttatttt ctgccattcc cccgatttat tcttcctttt tgtcctgttt
     9601 cagcagatta tcaatatttt ccagataatc caggctgtcg tcaatgtgaa aatagagttc
     9661 gggaatgatg cgcaactgat gacgaacccg ttttcccaaa tcaaagcgaa tggattttgt
     9721 atcggcatta acctgttcca gcactgattt cactttatca gccggaaaaa tgctgagatg
     9781 tgcacgggcc acactcaaat cggggctgat ccgtactccg gttactgtaa tcagcgttcc
     9841 ctgcatcttt ttggcataga gtaaaaatat ttcacccaat tccttttgaa gcagtcgcgc
     9901 tattttttgt tgtcttgttg tttccataat ttagtttttt atattttcca ctcaaatccg
     9961 tccttggtat cctttatttc aaaaccaagc gctgtcagct cgttacggat cttatcactg
    10021 gttgcccagt ctttgttttt cttggcttcc aaccggatat ttaaaagtaa atcaattgct
    10081 ttatggtatg cttccgtact ttcagttgct tcattttgtg atttcaaacc cagtacatct
    10141 tctatgaaaa gtccgaaaac agactttaac tcatccagat cggcagctga aattgtttct
    10201 ttaccatcat gtaccagatt gatggctttc agcgcttcaa acaagtgcga aatcacta
//
LOCUS       DAOPYB010000106        10204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_7664_length_10204_cov_9.576510, whole genome shotgun sequence.
ACCESSION   DAOPYB010000106 DAOPYB010000000
VERSION     DAOPYB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7664_length_10204_cov_9.576510"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..383
                     /gene="rplL"
                     /locus_tag="PKH58_08745"
     CDS             9..383
                     /gene="rplL"
                     /locus_tag="PKH58_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNX89156.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAQILKDEYGIEPAAAAVAVAAG
                     PAAAGPVVEEKTAFDVVLKAAGANKLAIVKLVKELTGLGLKEAKDLVDAAPSAIKEGV
                     TKDEAEALKKSLEEGGAEVELK"
     gene            502..4311
                     /gene="rpoB"
                     /locus_tag="PKH58_08750"
     CDS             502..4311
                     /gene="rpoB"
                     /locus_tag="PKH58_08750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNX89157.1"
                     /translation="MSSNNNQRINFASIKNQMDYPDFLEVQLKSFQDFFQMDTAPEKR
                     KTEGLYKVFTENFPIADTRNNFVLEFLDYFIDPPRYSIEECFERGLTYSVPLKAKLKL
                     YCTDPDHEDFDTVIQDVYLGPIPYMTPKGTFVINGAERVIVSQLHRSPGVFFGQSMHA
                     NGTKLYSARIIPFRGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFESDKDILEI
                     FNLAEEVKVNKTNLKKVVGKRLAARVLKTWNEDFVDEDTGEVVTIERNEVIIDRETVL
                     ENNHIEEIIESGTQAVLIHKEDVNQSDYAIIYNTLQKDPSNSEKEAVLYIYRQLRNAE
                     PADDSSAREVINNLFFSEKRYDLGDVGRFRINRKLNLSTNSDIKVLTKEDIIEIIKYL
                     VELINSKAEVDDIDHLSNRRVRTVGEQLYNQFGVGLARMARTIRERMNVRDNEVFTPI
                     DLINAKSISSVINSFFGTNALSQFMDQQNPLAEITHKRRLSALGPGGLSRERAGFEVR
                     DVHYTHYGRLCPIETPEGPNIGLISSLCIYAKINELGFIATPYRTVNSSKVDLTPEGI
                     KYYTAEEEEDLVVAQGNAPLTDDGSFIRKVVKTRLEADFPVVPPSEVNLMDVAPSQIA
                     SIAAALIPFLEHDDANRALMGSNMMRQAVPLLRCDAPIVGTGIEGQLIRDSRTQVTAE
                     GEGVVEFVDATTIMVRYSRTEEEEFVSFDSAVKTYNLPKFQKTNQNTTIDLRPIVTKG
                     QKVNYGQIMTEGYSTHNGELALGKNLKVAFMPWKGYNFEDAIVINERVVREDIFTSIH
                     VVEQLLEVRETKRGIEEFTSDIPNVSEEATKDLDENGIIRIGARIEPGDIIIGKITPK
                     GESDPSPEEKLLRAIFGDKAGDVKDASLKATPSLSGVVIGKRLFSKAQKNRKSKLADK
                     AVLPKLDEDFEAQAASLKDTLIEKLLIIIEDKTSAGVKDFLGADIVSRNSKFTAERLQ
                     EIDFTTIQLGKWTSDNHKNDLIRQLILNYLHKYKELDAQIKRQKFNITIGDELPNGIV
                     QMAKVYIAKKRKIRVGDKMAGRHGNKGIVSKIVRQEDMPFLEDGTPVDIVLNPLGVPS
                     RMNLGQIFETVLGWAGKELGVRFATPIFDGASLDDLDQWCDKAGVPRYGKTYLYDGGT
                     GERFDQTATVGIIYMLKLGHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEV
                     WALEAFGASHILQEILTVKSDDVMGRARTYEAIVKGDPMPTPGIPESLNVLLHELRGL
                     GLSINLE"
     gene            4430..8728
                     /gene="rpoC"
                     /locus_tag="PKH58_08755"
     CDS             4430..8728
                     /gene="rpoC"
                     /locus_tag="PKH58_08755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNX89158.1"
                     /translation="MAFKIDNKSKSSFNKISIGLASPEEILRLSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPTKDFECHCGKYKRIRYRGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRSLPNKIGYLLGMPTKKLDAIIYYEKYVIIQAGILDNGENIVNNELL
                     TEEEYLNLLDTLPRENQLLDDSDPNKFIAKMGAEAIYDLLTRLDLDSLSYELRNRANT
                     DTSQQRKTEALKRLQIVESFRASKNRNKPEWMIVKIVPVIPPELRPLVPLDGGRFATS
                     DLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLLDNSRKSSAVKTDAN
                     RPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKP
                     FVIRKLIERGIVKTVKSAKKIIDRKDPVIWDILEYVMKGHPVLLNRAPTLHRLGIQAF
                     QPKMIEGKAIQLHPLACTAFNADFDGDQMAVHLPLGNEAVLEAQMLMLASHNILNPSN
                     GAPITVPSQDMVLGLYYITKPRPGSKGEGLIFYSPEEAEIAYNEGVVSLHSLIKVKTR
                     DLDENGEQTIRLIETTIGRILFNKHVPVEMGYINELLSKKSLRDIIGRVIDACGVART
                     AQFLDDIKDLGYYMAFRGGLSFNLSDVIIPKEKETLVQEGNAEVEEIMNNYNMGFITP
                     RERYNQIIDTWTHVNTKLSNVLIKQLAEDRQGFNSMYMMLDSGARGSKEQIRQLAGMR
                     GLMAKPQKSGAEGGQIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYL
                     TRRLVDVSQDVIINEEDCGTLRGLIATEIKNNEDVISSLYERILGRVSVHDIYHPLTG
                     ELLVASGEQIVETAAKKIQDSPIERVEIRSVLTCESKKGVCAKCYGRNLATGKMVNQG
                     EAVGVIAAQSIGEPGTQLTLRTFHVGGIASNIAAESKITLRYDGRLEFDELRTVDSID
                     EEGTECRVVVSRLTELRVVDPNTGIVLTTQNVPYGSKLYAKHGDLGRKGQFVCSWDPF
                     NAVIIAEAEGKIEFEDVIENVTYKTEVDDATGLREKIIIESKDKTKVPSANVVDNEGN
                     IRNYNLPVGAHLVLDDGSTIKAGQMLVKIPRAVGKAGDITGGLPRVTELFEARNPSNP
                     SVVAEIDGEINFGKIKRGNREVSVTSKTGEVKKYLIPLSKQILVQENDFIRAGTPLSD
                     GAITPADILAIKGPTAVQDYIVNEVQDVYRLQGVKINDKHFEVIVRQMMRKVEIDDQG
                     DTRFLEKQIVDKNEFMEENDRIWGKKVIIDAGDSDTLKPGQIITARKLRDENSVLKRK
                     DLKLVQTRDAVPATSSQVLQGITRAALQTNSFMSAASFQETTKVLNDAAINGKIDKLE
                     GLKENVICGHLIPAGTGLREYEKIIVGSRDEYEKMMDARKNVLDINREDEMEVE"
     gene            9015..10088
                     /locus_tag="PKH58_08760"
     CDS             9015..10088
                     /locus_tag="PKH58_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNX89159.1"
                     /translation="MENNYCVIMAGGLGSRFWPFSRNDKPKQFLDFFGTGRSLIQMTY
                     DRFRALVPASNILIVSNLIYKDLILEQLPEIKPEQVLLEPARRNTAPCIAYAIQRIKA
                     TTKKANIIVAPSDHLIMKETEFLNTIREGLDFVDKNDCLLTLGIKPSRPETGYGYIQV
                     EEGTTNPRKVKTFTEKPNAELAQVFLETGEFFWNSGIFLWNLKAIDNAFKMYLPDVAN
                     KFNEGEKFYNTDKEQDFINEMYPSCPNISIDYGVMEKAESVYVLCADFGWSDLGTWGS
                     LYDLSQKDDMKNVTLKCEAIFYNSEDNIIALPNGKLAVIQDLNGYIVAESDNVLLVCK
                     KSDEQRIRQFVNDANVKFDGKYS"
BASE COUNT         3288 a         1863 c         2202 g         2851 t
ORIGIN      
        1 aaaataaaat ggcagatttg aaagcttttg cagaacaatt ggttaactta acagtaaaag
       61 aagttaatga gttagcccaa attttgaaag acgaatatgg tattgaacca gcagctgctg
      121 cagttgcagt tgccgcaggt cctgctgccg caggtccggt agttgaagaa aaaacagcat
      181 ttgatgttgt tttgaaagca gccggcgcta ataaattagc tatcgttaaa ttagtaaaag
      241 aacttaccgg tcttggcttg aaagaagcta aagatttagt agatgcagct ccttcagcaa
      301 tcaaagaagg cgttactaaa gacgaagctg aagccctcaa aaaatcactt gaagaaggcg
      361 gagctgaagt tgaacttaaa taaaaatccc tgattatcag gtctttggtt tagtttccct
      421 cgttatgagg gattctaaac ctttttctgt ctatacaaaa tattcagttc ttcgcactaa
      481 acaaacataa cctctttgac aatgtcttca aataataatc aaagaataaa ttttgcttcc
      541 atcaaaaatc aaatggatta tcctgatttt ctggaagtac aattaaaatc tttccaggac
      601 ttttttcaaa tggacacggc gcccgaaaaa aggaaaaccg aaggtttata taaagttttt
      661 accgaaaact tcccaattgc cgatacacga aacaattttg tattagaatt cctggattat
      721 tttattgatc ctccccgtta ttccatcgaa gaatgtttcg aacgcggttt gacatacagt
      781 gtccccctta aggcaaaact gaaattatac tgcactgatc ctgatcacga agatttcgat
      841 actgttattc aggatgtata tctgggacct attccttata tgacccccaa aggcacattt
      901 gtaatcaatg gtgccgaacg tgttattgta tctcagctcc accgttcgcc gggtgtattc
      961 ttcggtcaaa gcatgcacgc aaatggtaca aaactttatt ctgcgagaat aattccattc
     1021 agaggttcgt ggattgaatt cgccaccgac atcaataacg tcatgtatgc gtacattgac
     1081 cgtaagaaga aattaccggt aacaactctg ttacgggcta ttggttttga aagtgataaa
     1141 gacatactcg aaattttcaa tctggctgaa gaggtaaaag ttaacaagac aaatcttaaa
     1201 aaggtcgtag gcaaaagact tgcagcccgt gtgttgaaga cctggaatga agattttgtt
     1261 gatgaagata ccggtgaggt agtaaccatc gaacgtaatg aggtgataat tgaccgtgaa
     1321 actgttctgg aaaacaacca cattgaagaa attatagaat cgggaacaca agctgttctt
     1381 atacataaag aggatgtaaa ccaatcggat tatgcaatca tttacaatac tctgcagaaa
     1441 gacccgagta attcggaaaa agaagctgta ttgtatattt acagacaatt gcgtaatgcc
     1501 gaacctgctg acgactcttc agcaagagag gtaatcaata atttgttttt ctccgaaaag
     1561 agatatgacc ttggtgatgt gggacgtttc cgtattaacc gaaaactgaa cttatcaact
     1621 aattcggata tcaaggtact gacaaaagaa gatataatcg aaattatcaa atacttggtt
     1681 gagttaatca actcgaaagc agaagttgat gatattgacc acttaagcaa ccgtcgtgtg
     1741 cgtaccgtgg gtgaacaact ttacaatcag ttcggagttg gtcttgcacg tatggcacgt
     1801 accattcgtg aaagaatgaa tgtacgcgat aatgaagtat ttacgcctat tgacctgatt
     1861 aatgcaaaaa gcatatcatc agtaataaat tcatttttcg gaacgaatgc tctttctcag
     1921 tttatggatc agcagaatcc gttggctgaa atcacacaca agcgtcgttt atcagcctta
     1981 ggtcctggtg gcctttcacg tgaacgtgct ggttttgagg ttcgtgacgt acactataca
     2041 cattacggac gtctttgccc cattgaaaca cccgaaggtc caaatattgg tttgatatcg
     2101 tcactttgta tatatgccaa aattaatgaa ttaggtttta ttgctacgcc gtatcgaaca
     2161 gttaatagct caaaagtaga tttaacacca gaaggtatta agtattatac tgctgaagaa
     2221 gaagaagatc tggtagtagc tcagggtaat gctccattga cagatgatgg cagttttatc
     2281 cgcaaagttg taaaaacgcg tcttgaagca gatttccctg tagtgccacc atctgaagtt
     2341 aatttgatgg atgttgcacc ttcacaaatt gcatctattg cagccgcatt gattccgttt
     2401 ctcgaacatg atgatgctaa ccgtgcgttg atgggatcaa acatgatgcg ccaggctgta
     2461 ccattgctcc gatgcgacgc cccgattgta ggaacaggta tcgaaggtca gttaattcgt
     2521 gactcacgta ctcaagttac agctgaaggc gagggagtag tcgaatttgt ggatgctacg
     2581 accataatgg ttcgctattc gcgtaccgaa gaagaggagt ttgtaagctt tgattccgca
     2641 gtgaaaacct ataatctgcc aaaattccag aaaactaacc aaaataccac aattgacctg
     2701 cgtcccatag ttactaaagg acaaaaagtt aattatggac agataatgac tgaaggttat
     2761 tccactcata acggtgaatt agctttaggt aaaaacctta aggtggcatt catgccatgg
     2821 aaagggtata attttgagga tgccatcgta atcaacgaaa gggtagtacg tgaagatatt
     2881 ttcacctcca tacatgtagt tgagcagttg ctggaggttc gtgaaacaaa acgtggtatt
     2941 gaagaattta cttcagatat tccgaatgta agcgaagaag ctacaaaaga tttggatgaa
     3001 aatggtatta ttcgaattgg agcacgtatt gaacccggtg atattatcat tggtaaaata
     3061 actccaaaag gagaatcgga tccttcaccc gaagaaaagt tgttgcgtgc aatctttggt
     3121 gataaagccg gtgatgtaaa agatgcttca ttgaaagcta cgccatcgct ttcaggagta
     3181 gttattggta aacgattatt ctcaaaagca caaaagaacc gcaaatccaa actggcagac
     3241 aaagctgttt tgcccaaact ggatgaagac tttgaagcgc aggcagcttc actgaaagat
     3301 acgctgatag agaaattact catcatcatt gaagataaaa cctcagccgg agtaaaagac
     3361 tttttaggtg ctgacatcgt atcccgaaac agcaaattta ccgctgaacg tctgcaagaa
     3421 attgatttta ccactattca gttgggtaaa tggacatcag ataatcataa gaatgatttg
     3481 atccgtcagt taatactgaa ttatttacat aaatacaaag aactggatgc acagatcaaa
     3541 cgccagaagt tcaatattac cattggtgac gaacttccga atggtattgt tcaaatggca
     3601 aaagtttata ttgccaaaaa acgtaaaata cgcgtaggtg ataagatggc cggtcggcac
     3661 ggtaataaag gtattgtttc caaaattgta cgtcaggaag atatgccgtt cctcgaagac
     3721 ggaactcctg ttgacatagt gttaaatccg cttggtgtgc cttcacgtat gaaccttggt
     3781 cagatttttg aaaccgtact gggatgggca ggaaaagaat taggcgtgcg ctttgcaact
     3841 cctatttttg atggagcatc acttgatgac ctggaccaat ggtgcgacaa agcaggagtg
     3901 ccacgttacg ggaaaacata tctctatgat ggaggcaccg gtgagcgttt tgatcagaca
     3961 gcaactgtcg gtatcattta tatgcttaaa cttggccata tggttgaaga taaaatgcat
     4021 gctagatcca tcggtccata ttcacttata actcaacaac cactcggtgg taaagcacag
     4081 tttggtggtc aacgttttgg tgaaatggag gtatgggcgc ttgaagcttt cggcgcatca
     4141 cacattttgc aggaaatcct tactgtaaaa tcggatgatg ttatgggtcg tgcaagaacc
     4201 tatgaagcaa tcgtgaaagg tgatccaatg cctacacccg gaattccgga atcactcaat
     4261 gtattattgc atgaattgcg tggtttagga ttaagtatca atttggaata atctgaaaag
     4321 ccccctaact ttctctctga gtgaaaatta tgggaaaatc aatttagaga acttttttta
     4381 caaatgtgag aaaagtaatt tgtaaataaa taaattgtca atataaaaca tggctttcaa
     4441 aatcgataat aaatcaaaga gtagctttaa taaaatctct atcgggttgg catcgccgga
     4501 agaaatactc cgactttcga gcggagaagt gctgaaacct gaaaccatta attaccgtac
     4561 ctacaaacca gagcgggatg gattgttctg tgaacgaatt ttcggtccaa cgaaggattt
     4621 cgaatgtcat tgcggaaagt ataaacgtat ccgttaccgt ggcattgttt gtgaccgatg
     4681 cggtgtggaa gtaactgaga agaaagtccg tcgtgaacgt atgggacata ttcaactggt
     4741 tgttcccgta gcacatatct ggtatttccg ttcattgcct aataagatag gttatttgct
     4801 cggcatgccc acaaaaaaac tggatgcaat catttattat gaaaaatatg tgattataca
     4861 ggcgggtatt cttgataacg gcgaaaatat tgtaaataac gaactgctca cagaagaaga
     4921 atatctgaat ttgctggata cacttccacg tgaaaatcaa ttgctggacg atagtgatcc
     4981 gaacaaattc attgcaaaaa tgggtgctga agctatctat gatttgctca caagactgga
     5041 tctggattca ctttcttatg aattgcgtaa cagagcaaac accgatactt cacaacaacg
     5101 caaaactgaa gccctgaaac gccttcagat tgtagaatca ttccgggcat cgaaaaaccg
     5161 aaataaaccg gaatggatga ttgtgaagat tgtaccggtt attccacctg aattacgtcc
     5221 gttggtacca ctggatggtg gtcgttttgc cacatccgac ctcaatgatt tgtaccgtcg
     5281 tgttattatc cgcaacaacc gtctgaagcg tttgattgaa attaaagcgc cggaagtaat
     5341 tttacgtaac gaaaaacgta tgcttcagga agcagtggac tcattactgg ataattcgcg
     5401 taaatcaagc gctgtaaaaa ctgatgccaa tcgtccgctg aaatccttgt cagatagttt
     5461 gaaaggtaag caaggacgtt tccgtcaaaa tttgctgggt aaacgtgttg actattctgc
     5521 acgttcggta attgtcgttg ggcctgaact gaaaatgcac gaatgtggtt tgcctaaaga
     5581 tatggcagcc gaactatata aaccattcgt tatccgtaaa ttaattgaac gcgggatagt
     5641 aaaaacagta aaatcagcca aaaagattat tgaccgaaaa gatccggtca tttgggatat
     5701 tctggaatat gtaatgaaag gacatcctgt gttgctcaac cgtgccccga cacttcaccg
     5761 tttgggtatt caagcattcc agccaaaaat gattgaagga aaagcaattc agttacatcc
     5821 actcgcttgt acagctttca atgctgactt tgacggtgac cagatggctg ttcacttacc
     5881 acttggcaat gaagctgttc tcgaagctca aatgttgatg ttggcatcac acaatattct
     5941 taatccatcg aatggcgctc ctattaccgt tccatctcag gacatggtat tgggattgta
     6001 ttatatcact aagccacgac cgggatcaaa aggagaaggg ttgatttttt attcacctga
     6061 ggaagcagaa atcgcatata acgagggtgt tgtgtctctc cactctttaa ttaaggtaaa
     6121 aaccagagat ttggatgaaa atggagaaca gacaattcgc ctgattgaaa ccacaatagg
     6181 acgcattctg ttcaacaaac atgtgcccgt ggaaatgggt tacatcaatg aactgctatc
     6241 gaagaaatct ttgcgtgata tcattggtcg tgtgattgat gcttgcggtg ttgcacgtac
     6301 agcacaattc ctggatgata tcaaagacct gggatattac atggcgttca gaggaggtct
     6361 atcctttaac ctgagtgatg taataatacc aaaggaaaag gaaacactgg ttcaggaagg
     6421 taatgcagag gtggaagaaa tcatgaataa ctataatatg ggtttcatca ctccccgtga
     6481 acgttataac cagattatcg atacctggac acatgtaaat acaaagttgt caaatgtgtt
     6541 aataaaacag ttagctgaag accgtcaggg tttcaactcc atgtacatga tgcttgactc
     6601 cggagcccgt ggttctaaag aacagatacg tcagctggct ggtatgcgtg gtctgatggc
     6661 taaaccgcag aagtctggtg ctgaaggtgg acaaatcatc gaaaatccta tcttatcaaa
     6721 ctttaaggaa ggactttcag tacttgaata ctttatttcc acacacggtg ctcgtaaagg
     6781 tttggctgat acagctctga aaacagcaga tgccggttac ttaacccgtc gtctggtaga
     6841 cgtttctcaa gatgtaatta tcaatgaaga ggattgcggt acattacgcg gattgattgc
     6901 aactgaaatt aaaaataacg aagatgtaat ttcatccttg tatgaacgta ttttgggacg
     6961 tgtttctgtt catgatatct atcatccgtt gaccggtgaa cttcttgttg cttcgggcga
     7021 acaaatagtt gaaactgccg ctaaaaagat tcaggattct cctattgaac gtgtagaaat
     7081 tcgttcagta ttaacttgtg aatcgaagaa aggagtttgt gcaaaatgct acggacgcaa
     7141 cctggcaacc ggtaaaatgg ttaatcaggg cgaagctgtt ggcgttattg ctgctcagtc
     7201 tattggtgag ccgggtactc agctgacact tcgtacgttc cacgtcggtg gtattgcatc
     7261 aaacattgct gcagaatcaa aaattacgct acgttacgat ggccgtcttg aatttgacga
     7321 acttcgtact gtggattcga ttgatgaaga aggtacagaa tgcagggtag ttgtaagtcg
     7381 tttgactgaa cttcgtgtcg tagaccccaa tacaggaatt gtccttacta ctcaaaatgt
     7441 tccttatggt tcaaaactgt atgcaaaaca tggcgattta ggaagaaaag gtcagtttgt
     7501 atgtagctgg gacccattca atgctgttat tatcgcagaa gctgaaggta aaatcgagtt
     7561 cgaagatgta atagaaaatg ttacctataa aactgaagta gatgatgcta caggacttcg
     7621 tgaaaaaatt atcatagaat caaaagataa aacaaaagta ccaagtgcca atgttgttga
     7681 taatgaaggt aatataagaa actataactt acctgtagga gctcaccttg tgcttgatga
     7741 cggaagtact atcaaggctg gacagatgct tgttaagata ccacgtgctg taggtaaagc
     7801 cggggatatc acaggtggtc tgccacgtgt aacagaattg ttcgaagccc gtaatccatc
     7861 aaatccttca gttgttgctg aaattgatgg agagattaac ttcggcaaaa tcaaacgtgg
     7921 taaccgtgaa gtatcggtaa cttcgaagac aggtgaagtt aaaaaatatc ttataccact
     7981 gtcaaagcaa atacttgttc aggaaaatga ctttattcgt gcaggtactc cgctatctga
     8041 cggagcgatt acaccagccg atattctggc aatcaaaggt ccaactgctg tacaagatta
     8101 tattgtgaac gaagttcagg atgtttaccg tttacaaggt gtaaagatta acgacaaaca
     8161 ctttgaagtt atcgtacgcc agatgatgcg taaggtcgaa attgatgatc agggcgatac
     8221 ccgcttcctt gaaaaacaga tcgttgataa aaacgagttt atggaagaaa acgaccgtat
     8281 ctggggtaaa aaagtaatta ttgacgccgg agattctgat actttgaaac caggacagat
     8341 tattactgcc cgcaaactga gagacgaaaa cagcgtattg aaacgtaagg acctcaaact
     8401 tgttcagaca cgcgatgccg ttcctgcaac atcaagccag gtgttacagg gtataacacg
     8461 tgctgcattg cagacaaaca gtttcatgtc ggccgcttca ttccaggaaa caacaaaagt
     8521 actgaatgat gcagcaatca atggaaaaat cgacaagctg gaaggattaa aagaaaacgt
     8581 gatttgcggt catctgatcc ctgccggaac cggtctgcga gaatatgaaa aaattattgt
     8641 aggttcgcgt gacgagtatg aaaaaatgat ggatgcacgt aaaaacgttt tggatatcaa
     8701 ccgagaggat gaaatggaag tagaataaac ttcttaaatt cttagtaaat aaagataaat
     8761 ggcacattct gaatggattg tgccatttgt ttctttatgt tggaaacttc ttgtaagcat
     8821 cagaactgac tgagatgaaa aatgaatttt tttcatttta tgaatatttc tcaacttagt
     8881 ttaaaaatca aacaaaaatt cctgctacaa aaatggtatg ctatgattta agtccttact
     8941 tgctcaatac aaaaaaatag tgtaatttag tgcaggcatt ttgaataaag tatttatctg
     9001 aaaatcataa atatatggaa aataattatt gcgtaattat ggctggtggg cttggctccc
     9061 gtttctggcc tttcagccgg aatgataagc ccaaacaatt cctcgacttt tttggcacag
     9121 gacgatcatt aatacaaatg acttatgatc gtttcagggc tttagttccc gcttccaata
     9181 ttttgatcgt ttcaaattta atctacaaag atcttatttt ggaacaactg cctgagatta
     9241 aacccgagca ggtgcttctt gagcctgcga gacgtaatac agccccgtgt attgcctatg
     9301 caatacaacg gatcaaagcg acaactaaaa aagcaaatat aatagtagcc ccttccgatc
     9361 atttaataat gaaggagacc gaattcctga atacaatcag agaaggttta gattttgtcg
     9421 ataaaaacga ttgtctgctc accttaggaa ttaaaccaag tcgcccggaa accggatatg
     9481 gctatattca ggttgaagag ggtactacaa atccgcgtaa ggtaaaaacc tttacggaga
     9541 aaccaaatgc cgaactggca caagtatttt tggaaaccgg agaatttttc tggaattcag
     9601 gtatctttct ttggaatttg aaagctatcg acaacgcctt taagatgtat ttacctgatg
     9661 tggctaataa gttcaacgaa ggagaaaaat tctataatac cgataaggaa caggatttta
     9721 tcaatgaaat gtatccttcg tgtccgaata tatccataga ctatggcgtt atggaaaaag
     9781 ctgaaagtgt atatgtgctt tgtgcagatt tcggatggtc tgacctcggt acctggggct
     9841 ctctgtacga cctgtcccaa aaagacgata tgaaaaacgt tactctgaag tgtgaagcaa
     9901 ttttttataa tagtgaagac aatataatag cccttcctaa cggaaaattg gcagtaattc
     9961 aggatttgaa tggatacata gtagccgaat ccgataatgt actactggtt tgtaaaaaat
    10021 cagatgagca gcgtattcgc cagtttgtta acgatgcaaa tgtgaaattc gatggaaagt
    10081 actcctgaag attataaaat tgcgcatttt tgctctcagg ttttatgtaa tttgtaaggt
    10141 gtattatgtg tgcagaatag ttgatattta tcagaaaatc caaacttaag taataataaa
    10201 ttaa
//
LOCUS       DAOPYB010000107        10090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_7806_length_10090_cov_8.220229, whole genome shotgun sequence.
ACCESSION   DAOPYB010000107 DAOPYB010000000
VERSION     DAOPYB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7806_length_10090_cov_8.220229"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKH58_08765"
     CDS             <1..794
                     /locus_tag="PKH58_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89160.1"
                     /translation="LVTTISAQTSYPNNYSGYRKGYVFMKNGDILKGKYIYSSDMKKL
                     EVISGNQVRIYASEDVERITKKSPLEMADTFHVFNQADFKPSRIFNFTEFGLLVGNQD
                     NTIKSPVIFHSSVNYSFTPLLSAGLGTGVEMYREAYLPVTGNVMYKLNNHRTSPYVSL
                     QAGYQIPVEGTRMTYYEVMPVSVNPGYLDYYYPYPYYQNSALNARGGFLLNPSMGFFH
                     QLSRGLGMGVSVGYRFQILNYTGDNDYRLEVNYNRLSVKLGFIFN"
     gene            804..>1900
                     /locus_tag="PKH58_08770"
     CDS             804..>1900
                     /locus_tag="PKH58_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89161.1"
                     /translation="MKQLNYIFLLVTVFAFSSCMDKYTEFYTANSPVYLSFEDLRSAV
                     KTSEPSDLYNPGKIYFKDNILFVVEKLKGIHVFDMSVPSSPKNLSFITVPGCIDIAIR
                     DNILYTDSYVDLVSVDISTINAVKEVGRAKNVFPYSVPPTNNELRVEEIDQTRGIVTG
                     WEITREKKELNYQNVPVYPVWWYYSSNYALSDATGGLKGGAETSSSGTASGFGKSGSM
                     ARFGLYDKYLYSVDNNAVYVFNIQNQTAPALINKQYLGWNIETMFMYDNQMFLGTNNG
                     MIIASLQNPLVPAQISTYSHITSCDPVIVDDGYAYVTLRGGQTCRNTTVNQLDVLKLS
                     TDYKTITPVAFYNLTNPHGLGIDGNTLFVCDG"
     assembly_gap    1901..2000
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2001..3005
                     /locus_tag="PKH58_08775"
     CDS             <2001..3005
                     /locus_tag="PKH58_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNX89162.1"
                     /translation="MVRNGAEGTTKTEMNTTLKLSGLTDAEINEYYRRMQTTLPGLDP
                     KTKLSIANSIWIRQSNDFTVKPAFLKTNTDYFNSYVKLMDFNQTWAVDTINSWAKRNT
                     NGLIPEVIKSIDPYTMMLLMNAIYFKGTWVTQFDKKATTESNFTNEAGQDMRVNMMHV
                     TDTFAYADDDQAQYLEMPYGNKAFSMTVVLPVQGKTITDVLAKLTAASFADITSKMEN
                     QKVHVILPRFKVKQTFELKKMLQDMGMKKAFQENAELDGIATLKPLFVSFVKHDTYVE
                     VTEEGTEAAAVTTVGVGTTSADPLERYFIADRPFLFVIREKSTGIIIFIGKMGSVEKF
                     "
     gene            3054..4274
                     /locus_tag="PKH58_08780"
     CDS             3054..4274
                     /locus_tag="PKH58_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNX89163.1"
                     /translation="MKTCLIAMLGLIVLAGCTNDTPSDQKEAAKIELSTTQRQRVVQD
                     NDFAFGLLRKTIEISEEKNVFMSPLSVSLALGMAMNGANGLTRSEIEQVLNFNGMTAD
                     EINEYYRLMQQTLPRMDPATKLSIANSIWYRNGFNIKSSFLKINSDYFNATSQGLDFS
                     KPSALETINGWCIDHTNKLIPKVLDEIDANTVLFLINAVYFKGIWVTKFKESDTREAD
                     FIDEDGKTVKVDMMQVKSDFGYYTDGEAQYLSMPYGNKAFSMIVILPDDGKTTRDILW
                     KLTAEKLSGTVKSMTTQELQLYFPKFKVKNRFQLKPMLLAMGMKQAFVPDADFSGITD
                     NKPLWIDFVQHDTYVDVNEEGTEAAAVTTIGFKDSAGAALLVNKPFLFVIRENSTGII
                     LFTGKIANPGKSTI"
     gene            4288..4746
                     /locus_tag="PKH58_08785"
     CDS             4288..4746
                     /locus_tag="PKH58_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89164.1"
                     /translation="MKIVKKIFFPFILILISCFTGCDNKVDWSDGCYKLKFKYNQAFE
                     ISERSAMQSADGQYLLRFDSVFTDSRCPQGMYCVWQGIAGARFTLSWDNSEPESFDLY
                     TWNNPGANWCDSASCKNLKISLLSLTPYPSIDIRNSYNNYKVRIIVTKQK"
     assembly_gap    4810..4909
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4910..5734
                     /locus_tag="PKH58_08790"
     CDS             <4910..5734
                     /locus_tag="PKH58_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89165.1"
                     /translation="YDYEQCSDKSKGVIVGWELKEKTGTYTKNRWWWWRGGMVDDAYM
                     SNVESGAKNSGNTGITGSMARFSLYNNYLFTVMNNTLAVFDLSNRAPVVATDSIYVGW
                     DVETIFSYKDYMFMGTPRGMLIYSVSNPVKPVYVSSFTHFYGCDPVVVENDTAFVTVR
                     SGNLCGQNSNELIVVDVSNIKQPKQIVSYSMTSPKGLGIDNGTLFLCDDGLKVYDARN
                     PMILMAKQLAHYKGMDGYDLIPFEHTLMMIADDGIYQYDYTDLKNIKQLSKLPIGQ"
     gene            complement(5925..7205)
                     /locus_tag="PKH58_08795"
     CDS             complement(5925..7205)
                     /locus_tag="PKH58_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNX89166.1"
                     /translation="MKNICFYFEIHQPLRLKRYRFFEIGQDHYYYDDFQAEERIRAMA
                     EQSYLPANRTIAEMIRSSNGKFKCAFSISGVALEQLEQYAPEVIDSFKELAQTGSIEF
                     LAEPYAHSLASLYDTDEFVRQINLHAEKLESLFGKKPTAVRNSELIYSNEIGEIVSKL
                     GYKTILIEQVKDVLGWKSPNYIYCHPSISKLKLLVRNNKLSDDISFRFSNRNWSEFPL
                     TSEKFIGKIAETPETESIFNVWMGYEALGFYQHPESGIFEFLKAMPYHAIEQGIGFVL
                     PSDAPKKSDTVDSLSYTHPTSWAESKDISVWNGNDLQQEALSKLYAVSERVHLCNDKP
                     LQHDWMLLQTTDNFRYMSHYDSFGTNYVSPYEAFTNYMNVLADFLDRVDAQYPTNIEN
                     EELNELLKTINNQEKEIERLESEIKKLKARKEKK"
     gene            complement(7239..8498)
                     /locus_tag="PKH58_08800"
     CDS             complement(7239..8498)
                     /locus_tag="PKH58_08800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX89167.1"
                     /translation="MKALMFGWEFPPHILGGLGTASYGLTKGMSQQPDMEITFVIPKP
                     HGDEDQSFLKIIGACNVPVVWKDNSWEHVNNRIGRFMHPDEYFWLRNGIKYDFTRIFT
                     NDLGCIGFSGRYPDNLIEEISNYEAVASVLAHQLEFDVIHSHDWLTYPAGVFAKRISG
                     KPLVIHVHATDFDRSRGKVNPTVYNIEKTGMDMADHIMTVSNLTRKIVIEKYHQDPAK
                     VTTVHNAVEPLEDVDSFVKTPRKDKIVTFLGRITMQKGPEYFVEAAYRVIQRTKNVRF
                     VMAGSGDMMDAMIDLAAKRGIADRFHFPGFLRGRAVHEMLAQSDVYIMPSVSEPFGIS
                     PLEAMQVGTPSIISYQSGCAEILSHVVKTDYWDIDAMADAIYSIVTNPAMYKSLHELG
                     LEEVNNIKWYDAGMKVRSIYNMVTGLH"
     gene            complement(8495..>10090)
                     /locus_tag="PKH58_08805"
     CDS             complement(8495..>10090)
                     /locus_tag="PKH58_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNX89168.1"
                     /translation="KERMLVSFEPRILIKYTLLEAHSPTTLRFKPFLAFRSVNELTYQ
                     NHLANTSYLELKNGISMKMYEGYPKLCMQFSKTNSYIHQPYWNKNIEYYKEQERGYAY
                     KEDLLVPGYFEMPIKKGESIIFSAGITEVSPRTLSSLWEQELTRRNKRTNMFSCLKNS
                     AQQFYKREGDKTYLLAGYPWFGARARDEFVSLVACTIVIGRMDYFDAIINTAIPEIER
                     FMEGEREDFKLLGIDEPDVFLLFIMAIQHFALHQTTEEAAKRFGKLVIKIIQFIRKQN
                     HPNLFLHNNGLLYVNGTEKPATWMNAVENGRPITPRTGYIVEINALWYNALKFASEIE
                     NVLGNEQSSDLLNYQAEIARDAFVKIFWNGTYLYDYVVDNYRDEEVRPNMLLAVALLY
                     SPLDKQQQKSIVDITTKELLTPKGLRSLSPKSGSYRPEYIGGQKDRDWNYHNGPVWPF
                     TFGAFASAYLRIYKKSGKSFIERMLNGFETEMTELCVGSLSELCDGNPPFKGHGAPSF
                     AMSVAEVLRVLDMLKKIDNEIAE"
BASE COUNT         2835 a         2027 c         2162 g         2866 t
ORIGIN      
        1 tgttggtaac tacaatcagc gcgcagactt cttatccgaa caattattcc ggttatagga
       61 aaggatatgt ttttatgaaa aacggcgata ttctaaaagg aaaatatatc tattcctcag
      121 atatgaaaaa actggaagta atttccggaa accaggtgag aatctatgcg tcagaagatg
      181 ttgaacgtat cactaaaaaa agccccctgg aaatggctga taccttccat gtattcaatc
      241 aggctgattt taaaccttca agaattttca attttacaga attcggcttg cttgttggca
      301 atcaggataa tacaataaaa tctcctgtaa tctttcatag ctctgtcaat tattctttta
      361 ctccattgct gtcagccgga ctgggaaccg gtgtggaaat gtaccgcgaa gcctatctgc
      421 cggttaccgg gaatgtgatg tataagttga ataatcatcg cacatcgccc tatgtgtcac
      481 tacaggcagg gtaccagata cccgttgaag gtacacgtat gacctattac gaagtgatgc
      541 ctgtatcggt aaatccgggt tacctggatt attactatcc gtatccttat tatcagaatt
      601 cggctcttaa tgcaaggggc ggatttctgc taaatccttc catgggattt ttccatcagc
      661 ttagccgggg attgggaatg ggagtgtcgg taggataccg ttttcagata ttgaattata
      721 ccggtgataa tgattaccgc ctggaagtaa attataaccg tttgtcagta aaacttggtt
      781 ttatttttaa ttaatcagat attatgaaac aactaaatta tatctttctg ctggttacag
      841 tatttgcgtt ttcatcctgc atggataaat atactgaatt ctataccgct aattcaccgg
      901 tttacctcag ttttgaggat ctgcgcagtg ccgtaaaaac ttccgagcca tccgatctgt
      961 acaatcccgg taaaatctac ttcaaagata atatcctgtt cgtagtagaa aaactgaagg
     1021 gaatacatgt gtttgacatg tcggttcctt ccagccctaa aaatctgagt tttattaccg
     1081 tgcccggttg tatagatatt gccatcagag ataacatatt atataccgac agttatgtcg
     1141 atctggtatc agtggatatt tccacgataa atgcggtgaa agaagtcgga cgcgcgaaga
     1201 atgtctttcc ctattctgta cctcctacca acaatgaact gagagttgag gaaattgacc
     1261 agacccgggg aatagtaacc ggctgggaaa taacacgtga gaagaaggaa ctaaactatc
     1321 agaatgtacc ggtatatccc gtatggtggt attacagcag taattatgct ttgagcgatg
     1381 caaccggtgg attaaaaggc ggagctgaaa cgagttcgtc cggaacagca tcgggcttcg
     1441 gtaaaagcgg ttcgatggcc aggtttggat tgtacgataa atacctgtat tcggtcgata
     1501 acaatgctgt ttatgtattc aatattcaaa atcagacagc tccggcactt atcaataaac
     1561 aatacctggg ctggaatatt gaaaccatgt ttatgtatga taatcaaatg tttcttggaa
     1621 ccaacaacgg aatgattatc gcgtcgcttc agaatccgct ggtgcccgcg caaatttcca
     1681 cttactcgca cattaccagt tgcgatccgg taatcgtgga cgacggttat gcttatgtaa
     1741 ccctgagggg tggacaaacc tgccggaata caacagtcaa ccaactggat gtgcttaaac
     1801 tttccacaga ttataagact attactccgg tagcttttta taatctgacc aatcctcacg
     1861 gactgggaat tgacggaaat acactgtttg tttgcgatgg nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn atggtacgca acggcgcgga aggaactacc aaaaccgaga
     2041 tgaacacgac cctaaaactg agtggtctga ccgatgctga aataaacgag tattaccggc
     2101 gaatgcagac aaccctgccc ggtcttgatc cgaaaaccaa actcagtatt gccaattcaa
     2161 tctggattcg ccaaagtaac gattttaccg ttaaaccagc gtttttgaaa acgaataccg
     2221 attatttcaa cagctatgtg aaattgatgg atttcaatca gacctgggcg gtagatacaa
     2281 ttaacagctg ggcaaaacgg aatacaaacg gattgatacc cgaagtgatt aaaagcattg
     2341 atccttatac catgatgctg ctgatgaatg ccatttactt caaggggacg tgggtaacgc
     2401 agttcgataa aaaagcaacc acggaaagca acttcaccaa tgaagccggt caggacatga
     2461 gagtaaacat gatgcatgtg accgatacct tcgcgtatgc cgatgatgac caggcacaat
     2521 acctcgaaat gccctacgga aacaaagctt tcagcatgac agtcgtttta ccggttcagg
     2581 ggaaaacaat taccgatgtt cttgccaaac tgacagccgc gtcatttgca gatatcacca
     2641 gcaagatgga aaatcaaaaa gtacatgtga tcttaccgcg gtttaaggtt aaacaaacct
     2701 ttgaactcaa aaagatgctg caggacatgg gaatgaagaa agcgtttcag gaaaacgccg
     2761 aactggatgg tatcgccacg ttaaagcccc tgtttgtgag ctttgtgaag catgatactt
     2821 atgtagaagt aaccgaagaa ggaacggaag ctgccgctgt aacaaccgtc ggagtaggta
     2881 caacctcagc agatcctctg gaacgctact ttatagccga tagaccattc ctttttgtta
     2941 tccgtgaaaa aagcaccggc atcattattt tcataggcaa aatgggaagt gttgaaaaat
     3001 tctgatacag aagagtcgtt ttatttcctg agaataaata aaaattaaag tttatgaaga
     3061 cttgtctgat agcaatgctc ggtcttattg ttctggcagg atgtacgaat gatactcctt
     3121 ctgaccagaa agaagccgct aaaattgaac tttccactac acaacgacaa cgtgtggttc
     3181 aggacaatga ttttgctttc gggctgctcc gcaagacgat tgaaatttcg gaggagaaga
     3241 atgtgttcat gtcaccgctg agtgtaagcc tggcgctggg tatggctatg aacggtgcca
     3301 acggactgac acgcagtgaa attgaacagg tactcaattt caacggaatg actgccgatg
     3361 aaataaatga atattaccgc ctgatgcaac aaacactgcc tcggatggat cccgctacga
     3421 aactgagtat agctaactca atctggtaca gaaatggatt taatataaaa tcttctttcc
     3481 tgaaaattaa ctctgattac tttaacgcca cttcgcaggg actcgacttt tcgaagccgt
     3541 ccgcccttga aaccatcaac ggttggtgca ttgaccatac taacaaactc attcctaagg
     3601 tactcgatga aattgacgca aatactgttc tgtttttaat caatgcagtt tatttcaaag
     3661 gtatctgggt aaccaaattc aaagaatcgg atacccgtga agcagacttt attgatgagg
     3721 acgggaaaac tgtaaaagta gatatgatgc aggtgaagtc ggacttcgga tattacaccg
     3781 acggtgaagc tcaatacctc agtatgcctt acggcaataa agccttcagt atgattgtaa
     3841 tactgcctga tgatggaaaa actacccgag atatactctg gaaactcact gctgaaaaac
     3901 tgtcgggtac ggtaaaaagt atgacaacac aggaacttca gctttatttc ccgaaattta
     3961 aggtcaaaaa ccgctttcag ctcaaaccga tgctgctggc gatgggaatg aaacaggcgt
     4021 ttgttccgga tgccgatttt agtggaataa ccgataataa accgttgtgg attgactttg
     4081 tgcaacacga tacgtatgtg gatgtgaacg aagaaggaac agaggccgca gccgtaacta
     4141 ccattggatt taaggattcg gcaggggctg ctttactggt taataaacca ttcctttttg
     4201 taatacgtga gaatagtaca ggaattatcc tctttacagg gaaaattgca aatcccggga
     4261 aaagtacaat ataaacactt aattcaaatg aaaatagtaa aaaagatatt ttttccattc
     4321 atcttgattc tgatatcttg tttcaccgga tgcgacaata aagtcgattg gtctgatggt
     4381 tgttataagt tgaaattcaa atacaatcag gcatttgaaa tatccgaaag atcagctatg
     4441 caaagtgcgg atggacaata cctgcttcgt tttgattccg tttttactga ctcgcgctgc
     4501 ccgcaaggta tgtattgtgt atggcaggga attgccggtg cacgttttac gctctcctgg
     4561 gataattctg aaccggaaag ttttgatctc tatacctgga ataatccagg ggccaactgg
     4621 tgcgattctg cttcctgtaa aaacctgaaa atcagtttac tgtcattaac tccataccct
     4681 tcgatcgata tacggaatag ttataacaac tacaaagtca gaattattgt aaccaaacaa
     4741 aaataggaaa ttatgaataa actgaattat ttgtttcttt ttacgggctt gcttatgctg
     4801 aatgcctgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt atgattacga
     4921 acaatgttcc gataaaagta aaggggtgat tgttggctgg gaactaaagg aaaaaaccgg
     4981 aacctatacc aaaaaccgct ggtggtggtg gcggggagga atggttgatg acgcgtatat
     5041 gtcaaatgtt gagagtggcg caaaaaatag cggaaatacc ggcattacgg gttcgatggc
     5101 acgtttttca ctctacaaca actacctgtt tactgttatg aataacaccc tggctgtttt
     5161 tgacctgagc aatcgcgcgc ctgtagtagc aaccgacagt atttatgtgg gatgggatgt
     5221 ggaaaccatt ttcagctata aggattacat gtttatgggg actccccgcg gaatgctgat
     5281 atattcggtc agcaatccgg taaaacctgt ttatgtctcc tctttcacac atttttacgg
     5341 atgcgatccg gtggtggtag aaaatgatac ggcctttgtg acagtacgct ccggaaactt
     5401 atgcggacaa aattcaaatg aactgatcgt agtggatgta agtaacataa aacaacccaa
     5461 acagattgta tcctattcca tgacaagtcc caaaggactc ggcattgaca atgggacact
     5521 attcctgtgt gacgacggac tgaaagtgta tgatgcccgc aatccgatga tactgatggc
     5581 aaaacagctg gcacactaca agggaatgga cggatacgat ttaattcctt tcgaacatac
     5641 cctgatgatg attgccgacg atggtattta ccagtatgat tataccgatt taaaaaatat
     5701 taagcaattg agcaaacttc cgataggaca atagatattt actttctgca actcaatcat
     5761 cccgccgcag tgatgcatcg gggtgatttt ttttgacaat atctttcata taactgtatg
     5821 caactgtata ttaacttata ttttattcta tcgattattc caaaaaacaa accctgccat
     5881 atttttccgg cagggtttat gtttatttcc aagaaaataa ttgcttattt cttttctttt
     5941 cgtgctttta gtttttttat ttcactttcc agcctttcaa tctccttttc ctgattattg
     6001 attgttttca gcagttcgtt caactcttca ttttcgatgt tggttggata ttgcgcatcc
     6061 acgcggtcaa gaaaatcagc caatacattc atgtagtttg taaaagcttc gtagggagag
     6121 acatagtttg tgccaaatga atcgtaatgt gacatgtagc ggaaattatc ggtggtctgt
     6181 aaaagcatcc agtcgtgttg cagcggttta tcgttacaca gatgtacacg ttcactgaca
     6241 gcgtagagtt tactgagcgc ttcttgctgc aggtcgttac cattccagac actaatgtcc
     6301 ttgctttcgg cccagctggt ggggtgcgtg tatgacagcg agtctacggt atccgatttc
     6361 ttgggagcat ccgatggcaa aacaaatccg ataccctgtt cgatggcatg gtaaggcata
     6421 gctttaagaa attcgaatat acctgattca ggatgttgat agaagcccag tgcttcgtaa
     6481 cccatccaaa cgttgaaaat actttccgtt tcaggtgttt cagctatttt accgatgaat
     6541 ttttcggagg taagcgggaa ttcagaccag ttacgattag agaaacggaa tgaaatatcg
     6601 tcgctcagtt tattgtttcg aaccaagagt ttcagtttgg aaatggatgg atgacagtag
     6661 atgtaattcg gacttttcca tccaagtaca tctttaactt gttcgataag aatagtttta
     6721 tagcccagtt ttgaaacgat ttcaccaatc tcgttagaat aaataagttc tgagttacgt
     6781 acagcagtag gctttttccc aaataatgat tctaactttt cggcatgaag gttgatttgt
     6841 cgcacaaact catccgtgtc ataaagcgaa gcaagtgaat gggcataagg ctctgccaga
     6901 aactcaatgc tgccggtttg ggccagttcc ttgaaactgt caatcacttc gggtgcatac
     6961 tgttcgagtt gttcgagtgc cactccggaa atggaaaatg cacatttgaa ttttccgttt
     7021 gaacttcgta tcatttctgc tattgtacgg ttggcaggca gatatgactg ttcggccata
     7081 gcgcgaattc gttcttcagc ctgaaaatca tcatagtaat aatgatcctg cccgatttcg
     7141 aaaaaacggt agcgtttcag acgcagcggc tggtgtattt caaaataaaa acaaatgttc
     7201 ttcatatatc ttgattttat atttttctct tagaattatt aatgcaagcc tgtaaccatg
     7261 ttgtagatgg aacggacttt cataccggca tcgtaccatt taatgttgtt gacctcttcc
     7321 agtccaagtt cgtgaaggct tttatacatg gccggattag ttacaatgct gtaaatagca
     7381 tccgccatcg catcaatatc ccagtagtcg gtttttacaa catgactgag gatttcagca
     7441 cagcccgact ggtacgaaat aatagaggga gtgcctacct gcatggcttc gagcggcgaa
     7501 ataccaaaag gctcggaaac cgacggcatg atatatacat cgctttgggc aagcatttcg
     7561 tgaacggcac gtccgcgaag aaaacccgga aaatgaaacc gatccgcaat gccccgttta
     7621 gcggcaagat ctatcattgc atccatcata tcgccgctgc cagccattac aaaacgtacg
     7681 tttttggttc gctgaataac ccggtaagct gcttccacaa agtattccgg acctttctgc
     7741 atggtgatac gacccaaaaa ggtaacgatt ttatctttgc ggggtgtttt cacaaaacta
     7801 tcaacatctt caagtggttc cacagcatta tgcacagtag ttactttagc cggatcctga
     7861 tgatattttt ctatcactat tttacgggtc aggttgctca cagtcatgat gtggtcagcc
     7921 atatccattc ccgttttttc gatgttgtac actgtcggat tcactttacc gcgactacgg
     7981 tcgaagtcgg tggcatgtac gtgtataacc agtggtttac ccgaaattcg tttggcaaaa
     8041 actccggcag gataggtgag ccagtcgtgt gagtggatca catcaaattc gagctgatgc
     8101 gccagtacgc ttgccactgc ctcgtagttg gatatttctt ctatcagatt gtcggggtag
     8161 cgtcccgaaa atccgataca gcccagatcg ttggtaaaaa tgcgtgtaaa gtcgtattta
     8221 attccattcc gcagccagaa atattcatcg gggtgcatga atctgcctat tcggttgttg
     8281 acatgttccc acgagttatc tttccagaca accggaacat tgcacgctcc gataatcttc
     8341 agaaaactct gatcttcatc gccgtgtggt ttgggtatga caaacgtaat ttccatatcg
     8401 ggttgttgcg acataccttt tgtaagtccg taacttgcag tacccaaacc gcctaaaata
     8461 tggggaggaa actcccatcc aaacatgagt gctttcattc ggctatttcg ttatcaattt
     8521 ttttcaacat atccaatact ctcaatactt cggctacact catggcaaat gaaggagcac
     8581 cgtgcccttt gaatggtgga tttccgtcac ataattcgga gagtgatcca acgcaaagtt
     8641 cggtcatctc tgtttcaaat ccattgagca tccgttcgat aaacgatttg ccgcttttct
     8701 tgtaaatacg gaggtaagca cttgcaaaag ctccgaaagt aaaaggccaa acaggtccgt
     8761 tgtggtaatt ccagtccctg tccttttgtc caccaatgta ttccggtcgg tacgaaccgc
     8821 tttttggact tagtgaacgc aagcctttgg gagttagcag ttctttggtg gtaatatcca
     8881 cgatggattt ttgttgctgt ttgtcgagtg gcgaatatag caacgcaacg gcaagcaaca
     8941 tatttggccg tacttcttca tcacggtagt tatccaccac ataatcatag agataagtgc
     9001 cattccagaa tatcttcaca aaagcgtcgc gggcaatttc ggcctgataa ttgagtaagt
     9061 ctgacgattg ctcgttaccc agcacgtttt cgatttcaga ggcaaatttc agcgcgttgt
     9121 accatagtgc atttatttcc acaatgtagc cggtgcgcgg agttattggt ctcccatttt
     9181 caacggcatt catccatgtc gcgggtttct ctgtgccgtt gacataaagc agaccattgt
     9241 tatgtaagaa tagattgggg tgattctgct tgcggatgaa ctggattatt tttataacta
     9301 attttccaaa tcgttttgcg gcttcttctg ttgtttggtg cagcgcgaaa tgttggatgg
     9361 ccatgatgaa cagcagaaaa acgtcaggtt cgtcgatacc caaaagcttg aagtcttcgc
     9421 gttcgccttc cataaatcgt tcgatctcgg gtattgctgt gttgatgata gcatcgaaat
     9481 agtccattcg gccgataacg atggtacagg ctaccagtga aacgaattca tccctggcac
     9541 gagctccgaa ccacggataa ccagcaagca ggtatgtctt gtcgccttcg cgtttataaa
     9601 attgctgtgc agagtttttc agacacgaga acatattggt acgcttgttg cgccttgtga
     9661 gttcctgttc ccagaggctt gaaagtgtac gtggtgaaac ttcggtgata cctgctgaga
     9721 aaattattga ttctcctttt ttgattggca tttcgaaata tccgggtaca agcagatctt
     9781 ctttataggc atatccgcgt tcctgttcct tgtaatattc aatgtttttg ttccagtatg
     9841 gctgatgaat gtatgaattg gttttcgaaa attgcataca aagcttagga tatccttcat
     9901 acattttcat gctaattccg tttttcagtt cgaggtatga ggtattagcc agatggttct
     9961 gatacgtcag ttcgttgaca ctacggaatg cgagaaatgg tttgaaacgc aacgtagtag
    10021 gtgagtgagc ttcgagcagg gtatatttta tcagaattct tggctcgaag gatacgagca
    10081 ttctttcttt
//
LOCUS       DAOPYB010000108        10056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_7836_length_10056_cov_9.178682, whole genome shotgun sequence.
ACCESSION   DAOPYB010000108 DAOPYB010000000
VERSION     DAOPYB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7836_length_10056_cov_9.178682"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..2628)
                     /locus_tag="PKH58_08810"
     CDS             complement(145..2628)
                     /locus_tag="PKH58_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX89169.1"
                     /translation="MKRYKSILGFLFVLLPAILFAGECTSYSLKGNEVQFNCSDNTKL
                     SLKICSESVIKVWYDPKGVLEQNKPSFAVINDSLGISTKIEVSDQSACYELYTSKLRI
                     RVNKNPMQLQIFDKWQKLVFSDYKDQGHVSDAGKQIKKAYKTMRKDELFFGLGEKAGS
                     LNRRGREYKMWNSDKPCYSTTEDPLYKSIPFFLSNYQYGIFLDNTYKTEFKFGTESDD
                     YYSFEAPDGEFIYYFIFGKDYKEILSQYITLTGKPIMPPNWAFGFSQSRGLYTSEKQA
                     IEIASEFRKRQIPCDVIYQDIGWTQHLQNFEWRQGNYHNPREMVSKLKDKGFKMILSQ
                     DPVISKANNNQWTEADSLGFLVKDFRSGKSYDMPWPWGGNCGVVDFTNPEVANWWGKY
                     QQKPLDDGVKGFWTDMGEPAWSNEEDVDRLNMKHHAGMHDEIHNVFGLTWDKVVKEQF
                     EKRNPDKRIFQMTRAAYAGLQRYTFGWTGDSGNGNDVLEGWGQLANQIQIGISAGLGG
                     IPFWTTDISGYCGDITNYPAMAELYTRWMQFGVFNPLSRAHHEGDNAVEPWKFGETAE
                     TNCKAAIELKYQLFPFIYSYAREAHDTGLPINRGLFMEFPNDTEAIQTENQFMFGKEL
                     LIAPVVKKGERVKKVYLPEGEWIDFNNKQTKYSGGQSIMFNSPLNIIPMFVKKGSIIP
                     MMPVMQYIHEQKEYPLYVHIFPDNIGETASFDLYEDNGEDLGYLKDVFSRTLFQCTTT
                     ISNGYKIVITANDKGFKQADKRDMVLILNGIERKPLVIKSNGKKIKFVKQNKFNSDQE
                     AINWTWIKNTKECRIKIADSRINYSITIN"
     gene            complement(2649..4415)
                     /locus_tag="PKH58_08815"
     CDS             complement(2649..4415)
                     /locus_tag="PKH58_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 66 protein"
                     /protein_id="HNX89170.1"
                     /translation="MKKTIYLIVALTTLIVSGCSSDYFETTNNPLSYGESYIDITIST
                     DKALYNPGETVKFIAKGIPDGAKVRYTYLGNVISETDLSTETWSWTTPNDDYRGYMVE
                     IYTIENGVEKLINNIAVDVSSDWAKFPRYGFVSDYEKMDNKFISRNIDILNRYHINGL
                     QFYDWLYDHQRPLAGSVENPATSWEDIFYRTNYFSTVKSYIDEAKAKGMKTMFYNLCY
                     GATKNAASDGVSDEWYAYKDQNHSEKDYHDLSGGRSHIWVLDPGNEQWQAYLVARNKD
                     VYQALGFDGFHIDQLGYRGDRYDYNGKSIDMSKGYGSFINAMKTAEPDKRLLFNAVGG
                     YGQEAIANAPVDFLYVEVWGARDGDNPDMSYGDMIALMRDNVSKSNPEKNIVLAAYMN
                     YNVSSVGFVNKPGILLANSAIFAWGGSHLELGEHYLINEYFPSNNLQLKADLGKELIN
                     YYDFMVAYQNLLRDGGDFQAAEVAFTNSNIVATRWPAAKGQVATIGKKVNGKDIYHLI
                     NFTDANTMVWRDPKGTQAEPNLIDAPEVSVNVSGTVSKVWFASPDVNRGVAQNLQFVQ
                     TGNNVTVTLPSLKYWDMIVVEY"
     gene            complement(4429..5895)
                     /locus_tag="PKH58_08820"
     CDS             complement(4429..5895)
                     /locus_tag="PKH58_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HNX89171.1"
                     /translation="MKTIYRILISTVFLLPLLFVSCNEDLMETNKGETALKLKANTTD
                     LVLDVLNPNSSVLNFEWTTGTNNGTNAAISYQFQITNEDSNFESGITYELGKNTTSIV
                     YTNETLNSVLLDSLNAKSDSVITVKARVIATVLAAGTEPQISESFAVKIKTYKPIAKN
                     LYLIGGAAPNGWNADNATKMNSVNGTTGGFTWQGRLNAGELKFITKLGNFLPSYNKGV
                     EDNQLIYRETEDQPDNKFTISKSGQYKVTVNIITLNISIEELDAPEFGDLWFVGGFSG
                     WTFKEMRVDMLDPYIYHYNAELNSSNSSDEFKIATVNNFDPTTIFLRPAVNGQGAGTG
                     LSVVKWSESENTNDNKWKLAPGIYKITLNLRTMKVDIVPFTPFSMIYLVGDATPNGWD
                     IGNATAMDAVSGNSYKFTWTGHLNAKEIKFSCDRKTDWNGAFFLATSGGANPSGSEEQ
                     MLYSNVGSNPDNKWNITEAGTYTIELDQLQETVKFTKR"
     gene            complement(5933..7420)
                     /locus_tag="PKH58_08825"
     CDS             complement(5933..7420)
                     /locus_tag="PKH58_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX89172.1"
                     /translation="MKKLIYITTIFLSVLMVSCDDFLNKSPYEDPGAETIKDEASAIA
                     LTNAAYQPLQRPKLYNMRIWTLDIIAGNSQVGAGGGSDGIETVQLANFVADADNFATI
                     DFWRGPNPGILYCNTVLANVPEMDISESIKNRCIGEAKFLRAHYFFLLVQLYGDVPMS
                     LKPANAGDDLFPRRVGKDTIYNEVIIPDLKEAIKLLPTREEYSTIDKGRASKGAACGM
                     LAKVYLTLGKYVECIEMCDEVTKLGYTLNPDYSDCFGGEAKNKNTAESLFEVQYYGKT
                     KAGFFDDENQAAWHSTFMGPRNQEFVGGGYGWNQPTQEFVDQYETGDLRKDKTILYEG
                     CPAFGGKKYLSTMSMTGYNVRKFLVPLSISPDYNTNAANFVVLRYADVLLMKAEALNE
                     LGRTNDAVEPLYLVRKRAGLTNKSILQNLTQDQMRQRIIKERRVELAFEGHRWFDMIR
                     IDNGQYALDFFHSIGKVNAVRKHLLFPIPQKDIDTNPNLKQNPGY"
     gene            complement(7444..>10056)
                     /locus_tag="PKH58_08830"
     CDS             complement(7444..>10056)
                     /locus_tag="PKH58_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX89173.1"
                     /translation="VSVGSSVVKDKPVSNLAEALQGRAAGVMITNSGSPGSNSSIRIR
                     GLGSINDCSPLIVIDGVPTDLNLNAINQNDVETIDILKDASATAIYGSRGANGVVLIT
                     TKKGKSGQGVISFSANTAINNAIGTPKLLNASQFASLHNEMMLNGGQPQRPDFTDPTL
                     WGEGTNWYDALIQSGKMQNYLLSYSGGSDNNNYYVSAGIFDQKGIVINTDFKRYTFQF
                     NNEAKVRNWLKFGNNLTLSHDLKRNGSYNILGTMQSLPTQPIYNEDGSYSGPGNSAIW
                     YGDMRNPIGTANVEKSETKGYNLLGNIFGEINISKKVFLKSLVGVDYKDWSSVSFSPK
                     YDWKPIPAPTSTRSESWNKSITYLWDNTITYMDTFAEKHNINVMIGSSAQSNLFRYIN
                     GSKEDFLSDDYNQLNNGLLNPLIGGGQSEWALLSFFGRANYNYDNKYLLTATLRRDGT
                     SRISTNNRWGTFPSFSAAWRFTEESFYTKNKILTDGKLRIGYGETGNQSPLDPYAYIT
                     RLKTSQYVFNDTPVATLYPLVMPSPEIKWETVKQWNIGIDTHFFDQALSITLDAYLKN
                     TSNMLVGMAVPITTGYSDSYTPQINVGKVQNQGIELTISSKNIKSNNFEWITEANVSY
                     NKNKVLKLDGDVPMYFGVQTHTVGKPIGAYYGYVTNGIFQNWDEVNSYAVQKPSQAGG
                     QGTSPGDIRFKDLDNNGVINDNDRTFLGNPTPSWIFSMNNSFNFHNFDLQIYFQGVAG
                     NEIYNGTRSSLESMSTVTNQSIKMLDRWTGEGTSNSIPRAVYADPNNNNRVSDRFLED
                     GSYLRLKNITLGYTLPKKSLSKIYMSGARMFISAQNLLTLTRYSGFDPEVSGIDSGSY
                     PMTRTISLGLDIKF"
BASE COUNT         2927 a         2039 c         1684 g         3406 t
ORIGIN      
        1 agaaagtgaa gttgtcatca caagagtcat tataatgact gccattttaa gtaaagatgt
       61 tttcataaaa ataaaattta aagtgtttga aatttattta attatacaat ttgtattggg
      121 ttgttgttga aaaatttatt cttgctaatt aattgttatg gaatagttta ttcttgaatc
      181 agcaatttta attctacatt ctttggtgtt tttaatccac gtccagttta tagcttcttg
      241 gtctgaattg aacttgtttt gtttaacaaa tttaattttc tttccgtttg acttaattac
      301 tagaggcttt ctctctattc cgtttaatat caataccata tcacgtttat ctgcttgttt
      361 aaaaccctta tcgttggctg taatcacaat tttgtatccg tttgatattg ttgttgtaca
      421 ttgaaaaagt gttcgagaga atacgtcttt taggtatcct aaatcttcgc cattatcttc
      481 gtacaaatca aaacttgctg tttcgcctat attatcaggg aaaatgtgaa cataaagcgg
      541 atattctttt tgttcatgaa tgtattgcat aacaggcatc ataggtatta ttgagccttt
      601 ttttacaaac attggaataa tattcaatgg cgagttaaac ataatacttt gcccacctga
      661 gtatttagtc tgtttgttgt taaaatcaat ccattcacct tcgggcaaat aaactttctt
      721 aactcgctcc ccctttttta ctacgggagc aattaatagt tctttaccaa acataaactg
      781 gttttcagtt tggattgctt ctgtatcatt cggaaattcc ataaaaagcc ctctatttat
      841 tggtaagcca gtatcatgag cctcccgtgc atagctgtaa ataaaaggga acaattggta
      901 tttaagctca attgctgctt tgcagttagt ttcggcagtt tcgccaaact tccagggttc
      961 taccgcattg tcaccctcat ggtgtgcacg gctcaaaggg ttaaacactc caaattgcat
     1021 ccaacgtgta tataactccg ccatggcagg ataattggtt atatctccgc agtaacccga
     1081 tatgtcggta gtccagaaag gaattccacc caacccggcc gaaataccaa tttgaatctg
     1141 attcgccagt tgtccccaac cttcaagaac gtcatttcca tttccgctat caccagtcca
     1201 accaaatgtg tagcgttgta atcctgcata agctgcacgg gtcatttgaa atatgcgctt
     1261 atccggattt cgtttttcaa attgttcttt cacaacttta tcccatgtta atccaaatac
     1321 gttatgtatt tcatcgtgca ttcctgcatg atgtttcatg tttaatctgt caacatcttc
     1381 ttcattactc caagcaggtt cgcccatatc agtccaaaaa ccttttactc catcatccaa
     1441 tggcttttgt tggtatttgc cccaccagtt tgcaacttca ggattagtaa aatctaccac
     1501 tccgcaattt cctccccaag gccagggcat atcatatgat tttccgctgc gaaaatcctt
     1561 taccagaaaa cccagactgt ccgcttccgt ccattgatta ttatttgcct ttgaaataac
     1621 aggatcctgc gacaaaatca tcttaaatcc tttgtctttg agtttggata ccatttcacg
     1681 aggattatga taatttcctt gtctccactc aaaattttgc aaatgttgtg tccaaccaat
     1741 atcctgataa atcacatcac aaggaatttg tctttttcta aattctgaag caatttcaat
     1801 tgcctgcttt tcactagtgt aaagcccccg gctttgcgaa aatccgaatg cccaatttgg
     1861 aggcattatg ggtttgcctg tcagcgtaat atattgactt aatatttctt tataatcctt
     1921 gccgaaaata aaataatata taaactcacc atcaggagct tcaaatgaat aataatcatc
     1981 cgactctgtt ccaaacttaa actctgtttt gtaggtgtta tccaaaaata ttccgtattg
     2041 ataattactc aagaaaaagg gaatgctttt gtatagtgga tcttctgtag tactgtaaca
     2101 tggcttgtca ctgttccaca ttttatactc acgaccacga cggttcaatg accctgcttt
     2161 ttctcccaga ccgaaaaaaa gttcatcttt gcgcattgtt ttatatgcct ttttgatctg
     2221 tttaccagca tctgatacat gaccttggtc tttataatcg ctaaaaacca gtttttgcca
     2281 cttgtcaaat atctgtaatt gcatcggatt tttattaacc ctaatgcgca gtttgcttgt
     2341 atatagctca tagcaagcag attgatcgct tacttcaatt ttagtactaa ttcctaatga
     2401 atcattgatt acagcaaagg atggtttgtt ttgctctaaa acacctttcg gatcgtacca
     2461 tacttttatt accgactcgc tacatatttt aagtgataat ttagtattat cagaacagtt
     2521 aaactgaact tcattacctt ttaagctgta tgaagtacac tccccggcaa ataaaattgc
     2581 cgggagaagt acaaatagaa aacccaatat agatttatac cttttcatta agttatttcc
     2641 aactgtattt agtattcgac aactatcata tcccaatatt tcaatgatgg gagcgttacc
     2701 gtcacattgt ttcctgtttg aacaaactgt agattctgag ctactccgcg attaacatcg
     2761 ggtgaggcaa accacacttt agaaacagtg ccggaaacat ttacgcttac ttcgggggca
     2821 tctatcagat tgggttctgc ttgagttccc ttaggatcac gccaaaccat tgtatttgca
     2881 tcagtaaagt ttatcaggtg gtaaatatct tttccattta ctttttttcc aatagtagcg
     2941 acctgccctt tagctgccgg ccaacgagtt gcaacaatat tactgttggt aaaagccacc
     3001 tctgctgcct gaaaatcacc tccatcccta agcagatttt gataagcaac cataaagtca
     3061 taatagttta ttaattcttt gcccaaatct gcttttaatt gtaaattgtt gctggggaaa
     3121 tattcattaa tgaggtaatg ctcgcctaat tctaaatgtg atccacccca cgcaaaaatg
     3181 gctgagtttg ccaacaaaat tccaggtttg tttacaaagc caacactact cacgttatag
     3241 ttcatgtatg cggcaagtac tatgttcttt tcaggattac tttttgaaac attatcgcgc
     3301 attagtgcaa tcatatctcc ataactcata tcggggttat ctccatcgcg ggctccccaa
     3361 acttccacat aaaggaagtc gacaggtgca tttgcgatag cttcctgacc ataaccacca
     3421 acagcattaa acagcaagcg tttatccggc tctgccgttt tcattgcatt aataaacgaa
     3481 ccgtatcctt tactcatatc gatcgatttt ccattataat cgtatcgatc gccccggtaa
     3541 cccaactggt ctatatggaa gccatcgaaa ccaagcgcct gataaacgtc tttgttgcgc
     3601 gccactaaat aggcttgcca ctgttcgttt cccggatcaa gcacccaaat gtgacttcgt
     3661 cctcctgaaa gatcatgata atctttctct gaatgatttt gatctttata tgcataccat
     3721 tcatcgctta cgccatcgga tgctgcattt tttgtagcac cgtaacagag attataaaac
     3781 attgttttca tccctttcgc tttggcttca tcaatgtatg attttacagt agaaaaataa
     3841 tttgtacgat agaaaatatc ttcccacgaa gttgccggat tttcaacaga acccgcaagt
     3901 gggcgttgat ggtcgtataa ccaatcgtaa aattgcaatc cgttaatatg atatctattt
     3961 aaaatatcaa tattccggct aatgaatttg ttatccattt tttcgtaatc tgaaacaaaa
     4021 ccgtatcgcg gaaattttgc ccaatcggaa gaaacatcta ctgcgatatt attgattaac
     4081 ttttctacac cattttcaat ggtataaatt tccaccatat agcctctgta atcgtcattg
     4141 ggagttgtcc atgaccatgt ctcagttgat aaatcagtct cacttattac attaccaaga
     4201 tatgtgtagc gaaccttagc tccatctgga ataccctttg caatgaattt gaccgtttct
     4261 cccggattat atagagcttt gtcagtagag attgtaatgt caatatatga ttctccataa
     4321 gaaagaggat tatttgtggt ttcaaaataa tcgctgctac agcccgacac aattaatgtc
     4381 gttaaagcga caattaaata tattgttttc ttcatgattg atataatttt aacgtttggt
     4441 gaattttaca gtttcctgca attgatctaa ctctattgta taggtaccgg cttcggtaat
     4501 attccattta ttatcggggt ttgagcccac attgctgtac aacatttgct cctcgcttcc
     4561 gctcgggtta gctcctccac tggttgcaag gaaaaaagct ccattccaat cagtttttct
     4621 atcacaagag aatttaattt ctttagcgtt taagtgaccg gtccatgtga atttatacga
     4681 atttccactc acagcatcca tagcagttgc attgccaata tcccaaccat taggtgttgc
     4741 atcgcctaca agataaatca ttgagaatgg ggtaaatggt acaatatcta ctttcatggt
     4801 acgcaaattc agggttattt tgtaaatgcc aggtgcaagt ttccatttat tatcattggt
     4861 attctcactt tccgaccact tcacaacact taatccagtt ccagcacctt gtccatttac
     4921 tgctggacgt aaaaatattg ttgttgggtc aaaattattg acagtagcaa ttttaaattc
     4981 atcactactg tttgatgagt ttaattctgc gttataatgg tagatgtaag gatcaagcat
     5041 atccactctc atttctttga atgtccagcc ggagaatcct ccaacaaacc ataaatcacc
     5101 aaattcagga gcgtctaact cctcaatact aatgtttaaa gtgattatat ttacagtaac
     5161 cttatactgt cccgatttgc taatagtaaa tttattatcc ggttggtctt ctgtttcacg
     5221 ataaataagt tgattgtctt caacgccttt gttgtaggat ggtagaaagt ttccaagctt
     5281 cgtaatgaat tttagttctc cagcatttaa acgcccctgc catgtgaatc cacctgtcgt
     5341 tccattaaca gaattcattt tagtagcatt atcagcattc caaccattag gtgcagctcc
     5401 tccaatcaaa tagagatttt tagcgattgg tttataggtc ttaatcttaa ctgcaaaaga
     5461 ttcggaaatt tgaggttctg tacctgctgc aagaacggta gctattacac gcgctttcac
     5521 tgtgattaca gaatcggatt tggcatttag agaatctaat aaaactgaat ttaaagtttc
     5581 gtttgtgtat actatcgaag tcgtattctt tcctaattca taagtgatgc cagattcgaa
     5641 attgctatct tcgtttgtaa tttggaattg ataacttatt gcagcatttg taccattatt
     5701 tgtacctgtt gtccactcga aatttaaaac acttgaatta ggattaagta catctaaaac
     5761 caaatcagta gtatttgctt ttagtttaag cgcagtttct cctttgtttg tttccattaa
     5821 atcttcgttg cacgaaacaa aaagtaacgg caataaaaaa actgtggaga ttaatattct
     5881 atatatagtt ttcatcttat caaatttatt ttttgtttta acttcatgat tcttaatatc
     5941 ccggattttg tttcaaatta ggattagtat caatatcctt ttgtggaatt ggaaacagta
     6001 agtgtttgcg gacagcattt acttttccta tagagtgaaa gaaatccaat gcatattgtc
     6061 cgttatcaat acgaatcata tcgaaccagc gatgtccttc aaacgcaagt tctacgcgac
     6121 gttctttaat gattctttgt cgcatttgat cttgtgtaag gttttgtaag attgatttat
     6181 ttgttaatcc ggcacgctta cgcactaaat aaagtggttc tacggcatca ttagtacgtc
     6241 caagttcgtt caaagcttca gctttcatta gcagtacgtc cgcatagcga agtactacaa
     6301 agtttgctgc attggtgttg tagtcaggcg aaatagatag tggcacaaga aatttacgta
     6361 cattgtagcc tgtcatcgac atggttgaaa gatatttttt ccctccaaat gccgggcaac
     6421 cttcataaag aatggtcttg tcttttcgca aatctccagt ttcatactgg tctacaaact
     6481 cttgcgttgg ttgattccaa ccataaccac ctccaacaaa ttcctgatta cgtggcccca
     6541 taaaagtact atgccatgct gcctgatttt cgtcgtcgaa gaaaccggct tttgtcttac
     6601 cgtaatactg aacttcaaac aatgactcag cagtgttctt attcttagct tcgccaccaa
     6661 aacaatcact atagtcagga ttaagagtat atcctaattt agttacttcg tcacacattt
     6721 caatacattc tacatatttg ccaagcgtga gatatacttt tgccaacatt ccacaggctg
     6781 ctccttttga tgcacgacct ttatcgatag tactgtattc ttcccgtgtt ggaagcaatt
     6841 ttatggcttc tttcaaatct ggtataatca cttcgttgta aatggtatct tttcctacac
     6901 ggcgaggaaa taagtcatca cctgcgtttg caggctttaa gctcataggc acatctccat
     6961 agagctgaac caacaagaag aagtaatgtg cacgtaaaaa tttagcttcg ccaatacaac
     7021 gattcttgat actttcgcta atatccattt cagggacatt tgctaaaaca gtattgcaat
     7081 aaaggattcc cggattaggt cctctccaaa agtcaatagt ggcaaaatta tcagcatctg
     7141 ccacaaagtt tgctaattga actgtttcta tgccatcgct accgccaccg gcacctactt
     7201 gactattccc agctataatg tccaatgtcc agatacgcat attatataat tttggacgtt
     7261 gtaaaggctg gtaagcagca ttggtgagtg caatagcact tgcttcatct tttatggtct
     7321 ctgctcctgg atcttcataa ggtgacttat ttaagaagtc atcacatgac accattagaa
     7381 cagataaaaa tatcgttgtg atataaatta gttttttcat gattgcgtat tatttatatt
     7441 ggtttaaaat tttatatcaa gaccaaggct gattgttctg gtcatagggt aactaccaga
     7501 atcaataccc gaaacttcag ggtcaaaacc ggaataacgt gttaacgtaa gtaagttttg
     7561 tgcagaaata aacatacgtg caccagacat ataaattttg gacaacgatt ttttaggaag
     7621 cgtatatcct aaagtgatat ttttcaagcg caagtatgaa ccatcttcaa ggaatctgtc
     7681 agaaacacga ttattattat tcggatcggc atacactgcg cgtggaatgg agttactggt
     7741 gccttcgcca gtccatctat caagcatttt tattgattga ttagttactg tcgacatgct
     7801 ttcaagtgaa gatcgcgttc cattatatat ttcatttccc gctacaccct gaaaataaat
     7861 ttggagatca aagttgtgaa aattgaaact attattcata gagaatatcc aagaaggagt
     7921 agggttaccc aggaatgtcc gatcattatc atttataaca ccattattgt caagatcttt
     7981 gaatcgaata tctcctggag aagttccttg tcctcctgct tgagatggtt tttgtactgc
     8041 ataagaatta acttcatccc aattttgaaa gataccgttg gttacatagc cataatatgc
     8101 accaattggc ttccctactg tgtgtgtttg tactccaaaa tacattggaa catcaccatc
     8161 gagtttcaag actttatttt tgttatacga aacattcgct tcagtaatcc actcaaaatt
     8221 attactcttt atattcttag atgagatagt taattctatt ccttgatttt gtacttttcc
     8281 tacattaatc tgcggtgtat agctgtcaga atatcctgta gttatgggta ctgccattcc
     8341 tactaacata ttgctcgtgt ttttcaaata agcatctaaa gtgatactta aagcctgatc
     8401 aaaaaagtga gtatcaatac caatattcca ttgttttacg gtttcccatt taatctcagg
     8461 acttggcatt actaaggggt aaagagtagc aactggtgtg tcattgaaaa catattgtga
     8521 agtttttaat cttgtaatgt atgcataagg atctaacgga gactgatttc ctgtttcgcc
     8581 ataacctatg cgtaattttc cgtcagtaag aattttattt ttggtataaa atgattcttc
     8641 tgtaaaacgc cacgctgccg agaatgacgg aaatgttccc caacgattgt ttgtagaaat
     8701 acgagaagta ccatcgcgac gcaaagttgc tgtcaaaaga tatttattgt cgtaattata
     8761 atttgcacgt ccgaaaaaag acagcaatgc ccattcactc tgaccaccac caatgagcgg
     8821 atttagtagc ccattgttta gttgattgta atcatcggat aaaaaatcct ctttacttcc
     8881 atttatatat ctaaaaagat tactttgtgc actcgaacct atcattacat tgatattatg
     8941 tttttcagca aaagtgtcca tatatgtgat tgtattatcc cataaataag ttatagattt
     9001 attccaactc tcacttctgg ttgaagttgg agcaggtatt ggtttccaat catatttagg
     9061 agaaaatgaa actgaactcc aatctttata atcgactcct accaaagatt tcaagaaaac
     9121 ttttttagaa atgttgattt ctccgaaaat atttcccaac agattgtatc ccttagtttc
     9181 agatttttcc acattggcgg ttcctatcgg attacgcata tcaccgtacc agatagcact
     9241 attacctggt ccagaataac ttccatcttc gttatatata ggctgagtag gtagcgattg
     9301 catagttccc agaatattat aagaaccatt gcgttttaaa tcgtgactaa gtgttagatt
     9361 gtttccgaat ttcaaccaat tacgaacttt agcttcgttg ttgaattgaa atgtgtagcg
     9421 tttaaaatca gtattaataa ctataccttt ttgatcaaat atacccgctg agacataata
     9481 attgttatta tcacttccac cagaataaga tagcaggtaa ttttgcattt ttccgctctg
     9541 tataagagca tcataccaat tagtaccttc accccagagc gtagggtcag tgaagtcagg
     9601 tcgttgaggt tgtcctccgt ttagcatcat ttcgttatgc aacgaagcga actgactggc
     9661 attaagcaat tttggagtcc caatagcatt gtttattgca gtattagcgc taaatgaaat
     9721 tacaccttgt cctgatttcc ccttttttgt tgtaattaga actacaccgt ttgcgcctct
     9781 ggaaccataa attgcagttg cagatgcatc ttttagaatg tcaattgtct caacatcgtt
     9841 ttgatttatt gcatttaggt tcagatcggt tggaacacca tcgatgacaa ttaaaggaga
     9901 acaatcgtta attgatccca aaccacgtat acgaattgaa ctattacttc cgggtgaacc
     9961 tgaattagta atcatcacac cggctgcacg cccttgcagt gcttcagcaa gattcgaaac
    10021 aggtttgtct ttaaccacac tactccctac tgagac
//
LOCUS       DAOPYB010000109        10048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_7844_length_10048_cov_10.280997, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000109 DAOPYB010000000
VERSION     DAOPYB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7844_length_10048_cov_10.280997"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2251)
                     /locus_tag="PKH58_08835"
     CDS             complement(<1..2251)
                     /locus_tag="PKH58_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNX89174.1"
                     /translation="MTGREVIELIGLDRAKSVNSKGENLLESESIIMTQLKEQNFEVD
                     SVYFNTDENRNSFPAVFLKRVKTFNLETLYAIAETHKKIWNYKKVLFLYVYSETEIRI
                     YNCSEKPLIKTKEIFDYEKELQTVEIKSYQFSDKKQLEELGKLFSRIAIDSGIVWTLE
                     EAQFIRNKINLQRRVDKYLVESLVNTTEQLAKQGLKIDFIHKIILRSLFLLYLEDRGA
                     TDKNLYSLYKKGANSYFDILDDVDAAYKLYERLEDDFNGNVFTLEKGEIISADQLQLI
                     KKCFISGNDNTPQSKLFEEWRLFDFSIIQIELLSEIYENFLFKTDPALKKRTGTYYTP
                     PALVEFILNEKLPINKTVKNCNIKILDSSCGSGIFLVESFKRLVKRYENEHEEKLTDF
                     DTLKKLLTDNIYGIEIHPQAIKVAAFSLYLALVDKLDPKTLWQKKKHRLPNLINNPND
                     KSLSVQGKNLYCRDAILENKEIEDIEFNLVVGNPPFGTTDLLDSIRNYCDKENFAKEM
                     VLPFLHKATKFAPNGEIALIFNTKVLTNTGGTYQSFRKWLFNDCYVEKVFNFSILRNA
                     KKNFGGQLFGDATGPISIVFYQKEKPNEPSDKITYYAPKTFIKTNVIDGLSIDFTDLK
                     YLPREECQKPNTKIWKVAMWGGMNDWELIQRLDNSKLNSINKFAKKNNIETGVGFQLL
                     TQKKDKPKFSETLTKLKYLDADVITRYYTSDKLLRDVKKSIKTLKASKFYKDYYKVGD
                     INELEKLTAF"
     gene            complement(2704..4485)
                     /locus_tag="PKH58_08840"
     CDS             complement(2704..4485)
                     /locus_tag="PKH58_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89175.1"
                     /translation="MKFSGYRIKLAGLLIVVSAISGLKNESSAQDIFQLPFNEGWTSG
                     NFILNQWQAGVNWIIDGQQGSPKPAAKFKWDPALINYNSSIESWWLDDSSTNTTTYYS
                     IWFDFDVKLSDRTSSGTETLKAEVLKDSSWVVVAEFVNDSSFNWISKHIRITYKVRDK
                     AFKIRFTASGASSSQIYYWSVDNIHVYRDFPLSPPVNFVATLMAYNGYYMNDMYLTWS
                     PPVIPDVNPVWIHWDDGTNADAIGTGGPADFDIAARYDISQIEQYEGMSVTKIAFWPN
                     EATCQYSLRVWKGDNADSLLADQVVNNPVIGAWNEVELNTPVPIDVSEELWFGVRCNT
                     QYGYPAGCDNGPEIRDYGQWIFWEGTWQNLADLNSDLTFNWNLQAYLEQMDDSPHYQS
                     SPVISEIGRGLYPGQLHSQPARDTCIRTETNQTKHAGDIYQPDAFLGYNIYRRSYIWP
                     YTSTLTDWAKINDSPVSETYYWDMNLPKYCYSYYVIALYTEGVSVPSNIDNDNYWCTN
                     DVTEKDVDMAVISPVPASDHLRIDDPVGIRRLLMYNSKGKTITQQKLNGECLVICDVS
                     QFSPGMYFVMLERINSEISFYKILIAR"
     gene            complement(4963..5418)
                     /locus_tag="PKH58_08845"
     CDS             complement(4963..5418)
                     /locus_tag="PKH58_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89176.1"
                     /translation="MKQVHVECKPDELLVTKLGIHKRQVTHHQGKSRVFHALGRVSGQ
                     LAIVDEDPGSARTSYEKSLTFVEEIHGMRFYTDASNNKVCCLGGKLEDWIVRTSKHYK
                     IDLAAFRLPTDPDKLHDIINYRLSDFGGLLDTMLENKNPAMLKLVSFLR"
     gene            complement(5415..6404)
                     /locus_tag="PKH58_08850"
     CDS             complement(5415..6404)
                     /locus_tag="PKH58_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX89177.1"
                     /translation="MISKLTIRNFKSIRELTMQCRKLNIFIGEPNSGKSNIIEALCLQ
                     SQNSIGYNELNKELFRYKTLGDLFYDFNINTPVEVTTDDTQTLLSYAVNDDGSPENRF
                     HFNADSYTGKGNPTKIDHNGRIIDSGQNRKTNVRYYQYKRLESFSNNLLPHLSVPFGE
                     NMPSLLMANTEYKKWVSDFLKSVGLTLTLKPTENEITASKLVDGEIYSYPYSSLSETL
                     QRIIFYTLTLKSNKDCVILLDEPESNTFPMYTKILAERIALDMTNQFFITTHNPYLLL
                     NLIEKSPQDDINVCITTMRDYQTNITVLDPGQLSEVLDLNSDVFFNFDKLLNI"
     gene            complement(6508..7155)
                     /locus_tag="PKH58_08855"
     CDS             complement(6508..7155)
                     /locus_tag="PKH58_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89178.1"
                     /translation="MRYLLILAAVFLALSCSRKPGTIMPVDFGTFEKAMNSGSALTQQ
                     DVNKLMADLQEEVQKNTAGKQLPDVQVMDLPGNRFNLPDMIRRKTIIAVSDAHCGIGK
                     GCTMEDLPKIITRLKKQYDDFDVICLVVRTKADIDDPAAFKSYLAGLKPLYPVLCVME
                     EEAARKMNVFGSPTRLLIDKNRTVVKIKTGMAVDPEVLYDELAGFLMNVHSGLFQ"
     gene            7349..7765
                     /locus_tag="PKH58_08860"
     CDS             7349..7765
                     /locus_tag="PKH58_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89179.1"
                     /translation="MLDVPHVRIAEVCLRRHGTGVIWHGTGLILHGTGFIWHGTGLIL
                     HGTGFILRRTDFVMYGTGLILYGTGLILHGIGVVLHGTGLRLLRISLRLHGIGLILYW
                     TGSILHGTGSILHGTGFILHGTGFILHGLIYSVLDK"
     gene            8168..8866
                     /locus_tag="PKH58_08865"
     CDS             8168..8866
                     /locus_tag="PKH58_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89180.1"
                     /translation="MNGPGFLKRLALQRQVYFVPAGNVMGPFRNLAAIINTIREKLRA
                     FKDQQRQFAMVVSVLVDFGYSFSTVRLFYTRYSESLSSECTPALIRRYFREYLELVNS
                     ERNNFSHYGKYNFGKGNLSAVRDMSELIEFDIYMQSLSGYEGIGILNDAYELSPRQIS
                     FLESVSCINNCLSYVELLFLLVYLEIASLYLHLLALAAKLRKYRFSLSRQLSFAMITG
                     RPSLANSPPFMPGM"
     gene            9265..>10048
                     /locus_tag="PKH58_08870"
     CDS             9265..>10048
                     /locus_tag="PKH58_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX89181.1"
                     /translation="MKRLVSSRNKFITLQMIMNENQDAFEGFTNAIASRDKFISNTER
                     ISELLSLVDRPYTEIYHERKDASDRLVSALDDAIGIAISVAAKIDNAVAVRAMQSYRM
                     SIKAVSFNGISEISLRVIDFITANAEEFQASGFSAEQLSVLQQLSGALRDILQSTSFE
                     LSTRKTAKTELSSLASENRMILKVDLDNFVKSRRKFFPALYNAYMTERRIKRHKRRSS
                     GETTLCEISGTITDSATGMPLANAVVNLLSPETIETTDEDGVY"
BASE COUNT         2781 a         2091 c         1988 g         3188 t
ORIGIN      
        1 taaatgctgt tagtttttct aattcattaa tatcacctac tttatagtaa tctttataga
       61 acttgcttgc tttgagagtt ttgatagact ttttcacatc cctcagtaac ttatccgatg
      121 tgtaatatct tgttataaca tcagcatcta agtattttag cttagttaag gtttcgctaa
      181 atttgggttt atctttcttt tgggtgagca gttgaaagcc aacccctgtt tctatattat
      241 tttttttagc aaatttattt atagaattaa gtttagaatt gtccaaccgt tgtatcaatt
      301 cccaatcatt cattccgccc cacattgcaa ctttccaaat tttagtattg ggtttttgac
      361 attcttctct tggcagatat tttaaatcgg taaagtcaat actcaatcca tcaatcacat
      421 tagttttgat gaatgttttt ggagcgtaat atgtgatttt atcagagggc tcattaggtt
      481 tttctttctg ataaaacact atacttattg ggccagttgc atcaccaaat aattgccctc
      541 caaaattctt tttcgcattg cgaagaatgg aaaaattaaa aaccttttca acataacaat
      601 cattgaataa ccatttccta aaactttgat aagtcccgcc agtattggtt aaaactttcg
      661 tgttgaatat caatgcgatt tctccatttg gagcaaactt tgttgccttg tgcagaaatg
      721 gtaaaaccat ttctttggca aaattttctt tatcacaata gttgcgtatt gaatccaata
      781 aatcagttgt tccgaatggt ggatttccga ctactaaatt gaattcaatg tcttcgattt
      841 ctttattttc cagaatggca tctctgcaat acaaattttt gccctgcacg cttaatgact
      901 tatcgttcgg attattgata agattaggca aacgatgttt tttcttttgc cataatgttt
      961 ttgggtccaa tttatcaacc aatgccaaat acaaactaaa tgccgcaacc tttatagctt
     1021 gtgggtgtat ttcaattcca tatatgttat cggttaataa ttttttaagc gtatcaaaat
     1081 cagtcagttt ttcttcatgt tcattctcat aacgttttac taatcgttta aaactttcca
     1141 ctaagaaaat tccagaaccg cagcttgaat ccaaaatttt aatgttgcaa ttttttactg
     1201 ttttattgat aggtagtttc tcattcagaa taaactcaac taatgcagga ggagtataat
     1261 aagttccagt tcttttcttt aaagcagggt ctgttttgaa taagaagttt tcgtaaattt
     1321 cacttagcaa ctcaatttga atgatgctaa aatcaaacaa tctccattcc tcaaataatt
     1381 ttgattgcgg cgtattatca ttcccactta taaaacattt ttttatgagt tgaagttgat
     1441 cggcagatat tatttctcct ttttctaatg taaagacatt gccattaaaa tcatcttcta
     1501 atcgttcata aagtttgtat gcagcatcta catcatctaa aatgtcaaaa tatgaattag
     1561 cacctttttt ataaagtgag tataggttct tgtctgttgc tcctctgtct tcaagataca
     1621 ataggaataa tgaacgaaga ataattttat ggataaaatc gattttgagc ccttgtttag
     1681 caagctgttc agttgtattt actaaacttt ctaccaaata tttatcaacc ctacgttgaa
     1741 ggttaatttt gtttctaata aattgagcct cttccaaagt ccaaacaatt cctgaatcaa
     1801 tagcaattct ggaaaacagt ttcccgagtt cttcaagttg ttttttgtcc gaaaattgat
     1861 aagattttat ttcaacagtt tgcaattcct tttcataatc aaagatctct ttggttttaa
     1921 taagtggttt ttctgaacaa ttatagatgc gaatttcggt ttcggaataa acatataaga
     1981 acaatacttt tttgtaattc catatttttt tgtgagtttc ggctattgca taaagagttt
     2041 ctaaattaaa agtttttact cttttcaaaa acacggcagg aaagctattt ctattttcat
     2101 cggtgttaaa gtaaacagaa tcaacttcaa agttttgctc tttaagttga gtcatgataa
     2161 tagattcgct ttctaagaga ttttccccct tagaatttac ggatttggct cggtctaagc
     2221 caattaattc tattacttct cgcccagtca tttgattaaa aactaatcaa aaaattcctt
     2281 tcaatataca actgtttaat tcacaaaggt attttttttc aaaacttctt agaaatttca
     2341 atgtttgaat catgattttt aatcagtctg tcaggctttt ttcagactag cattggttaa
     2401 aaaacaagct ttttgggttt ttcagtgctg agttgtgtgt caggaatatc caatgtgtct
     2461 gtttgtgcaa tgggcttttc tttacttatc gttaagagca ggtatgcgca atatttcgtt
     2521 catagctcca aaaactggat aaacgcaaac gtattgaagg gtgaaaggta gggaattatt
     2581 tgggtttgag agcctgcctg ctatgcgatt gccggcgcgg ggcaggcagg tttgattctt
     2641 tgacaagctc aggataacaa tatgaaagct tgaatgctgg tatttgagat agtcaggaaa
     2701 ttattacctg gcaatcaata ttttgtaaaa tgaaatttcg ctgtttattc tttcaagcat
     2761 cacgaagtac attcccggag agaattgaga aacatcacat attaccaggc attcaccatt
     2821 taacttttgc tgtgtaattg tttttccttt cgaattatac attagtaatc tcctaatccc
     2881 caccggatca tcaatacgca gatggtctga agccggaacc ggcgagataa ctgccatatc
     2941 aacatccttc tcagttacat cgtttgtaca ccagtaattg tcattatcaa tattggaagg
     3001 aacagacact ccttcggtat acaatgcgat tacataatag gaataacagt atttcggcag
     3061 gttcatatcc cagtagtacg tttcagacac tggagagtca tttattttag cccaatcagt
     3121 aagtgttgat gtataaggcc agatatagga gcgacggtat atattatagc caagaaaggc
     3181 atcaggctga tagatatctc ccgcatgttt tgtctggtta gtttctgtcc ggatgcaggt
     3241 atctctggca ggttgcgaat gtaactgacc aggatataat cctcttccaa tttctgagat
     3301 aacaggcgat gattgatagt gaggtgaatc gtccatttgt tcgaggtacg cttgcaggtt
     3361 ccagttgaat gtcaggtcac tgttaaggtc ggcaaggttc tgccatgtac cttcccagaa
     3421 tatccactgt ccgtaatcac gtatctccgg gccgttatca cagcctgccg gatatccata
     3481 ctgggtgtta cacctgacac cgaaccaaag ttcctcagat acatcaatag gaacaggcgt
     3541 attgagttca acctcattcc atgcaccaat aacaggattg tttactacct ggtcagccaa
     3601 caggctgtct gcattgtctc ctttccatac cctcagcgaa tattgacagg tagcttcgtt
     3661 aggccagaat gcaatcttcg ttacagacat tccttcgtat tgctctattt gtgagatgtc
     3721 ataacgagca gcaatatcaa aatcagcagg gcctcctgtt cctattgcat ctgcatttgt
     3781 tccgtcatcc caatgaatcc aaaccggatt gacatcagga ataaccggtg gggaccatgt
     3841 caggtacatg tcattcatat aataaccatt gtatgccatg agcgttgcga caaaattcac
     3901 tggtggagat aaagggaaat cccgatatac atggatgtta tcaaccgacc aataatagat
     3961 ctggctggat gatgcacccg aggcggtaaa tcttatctta aaagctttgt ctctgacttt
     4021 ataagtgatc ctgatatgtt ttgatatcca gttaaaactg ctgtcgttta caaattctgc
     4081 aacaacaacc caactgctgt ctttgagtac ctcggccttc agggtctctg ttcctgaaga
     4141 agtccggtca ctgagtttca catcaaaatc aaaccatatc gaataatatg tggttgtatt
     4201 agttgatgaa tcatccagcc accagctctc aatagaagaa ttgtaattta ttaacgccgg
     4261 gtcccacttg aatttggccg caggtttagg gctgccttgc tgcccgtcga ttatccagtt
     4321 aacacctgcc tgccattgat tcagaatgaa gttgcctgaa gtccatcctt cgttaaacgg
     4381 gagctggaaa atatcctgtg ctgaagattc attttttaga ccggatatgg cagatactac
     4441 tatgagtaat cctgctaatt ttattctata acctgagaat ttcattttta gtagtttata
     4501 attctaataa taagtgatgt aatatttcag gtatttgagt ctgccatgtt taatgcagcg
     4561 aggataaggg tactgtatac tattatgaca attaacccgt tggataccac tacctggttt
     4621 taattttttt tcaccctgag ctttcattcc aactgctgct ttgcaatgca tgcatctgca
     4681 tccagatgag catttaccta acagagtggt ttcattgttg ctgccggata agtatttttt
     4741 agatgatcag gtcattataa aagtgggcac cattatacca gatacctgcc tttttaatac
     4801 tcataattgg ttaacaatgt cttgtcaggc agatacattc gcatctccgg agcggagaat
     4861 tgaagcatga gtttagatat catcatactt cggctgctga ccttcgctta acaattcatc
     4921 gactcaaaaa aggaatatcg cattaatatt gcattatatg atctacctga gaaaagatac
     4981 cagtttcagc atggccgggt ttttgttttc cagcattgta tcgagcaacc cgccaaagtc
     5041 gcttagcctg taattgatta tatcatgcaa cttgtcggga tcggtaggaa gcctgaatgc
     5101 tgcgagatca attttatagt gtttgcttgt ccttacaatc caatcttcca gtttgcctcc
     5161 caggcaacat actttgttat ttgaagcatc ggtataaaac cgcataccgt gaatttcttc
     5221 cacaaacgta aggctctttt cataactggt ccgggcgctg cctggatctt catctacaat
     5281 ggccagttgt ccgctcactc tgcccagcgc atgaaacacc cttgacttac cctggtgatg
     5341 tgttacctgc cttttatgta ttcccagctt ggttaccagc agttcatcgg gcttgcactc
     5401 tacatgaacc tgcttcatat attcaatagc ttatcaaaat tgaaaaacac atccgagttc
     5461 aggtccaata cctccgaaag ctgccccgga tcaagaacag tgatatttgt ctgatagtcc
     5521 ctcatggttg taatacatac attgatatca tcctgtggcg acttttcaat aagattgagc
     5581 aacaggtaag ggttgtgggt ggtaatgaaa aactggttcg tcatatccag ggctatccgt
     5641 tcggcaagta tcttggtata catgggaaac gtgttgctct cgggttcgtc aagcagtatt
     5701 acacaatctt tattgctctt taaagtcaat gtatagaaaa taatgcgctg aagggtttct
     5761 gacagggagg agtaaggata gctgtatatc tcgccatcga ccaatttact ggctgtgatt
     5821 tcattttcag tcggttttag tgttagcgtc aaccccaccg attttaagaa gtccgaaacc
     5881 catttcttat attccgtatt ggccatgagg agcgatggca tgttttcgcc aaacggaacg
     5941 gaaagatgcg gcagcaggtt gttagaaaag ctttccagcc gcttgtactg gtaatacctt
     6001 acgttggttt tccggttctg gcccgaatca atgatcctgc cgttgtggtc aattttagtc
     6061 ggattccctt tcccggtata tgagtcagca ttaaagtgaa agcggttctc aggtgaaccg
     6121 tcatcgttca cggcatagga aagcagggtc tgggtatcat cagtagtgac ttcaacaggt
     6181 gtgttgatat taaagtcgta aaaaaggtcg cccagcgttt tgtatctgaa aagttccttg
     6241 ttcagttcat tgtatccaat ggagttctgg ctttgcaggc aaagtgcctc aataatattg
     6301 ctcttgccgc tgttaggctc gccgatgaat atgttcagct tgcggcattg catagtcagc
     6361 tccctgatcg acttgaaatt cctgattgtt aacttgctga tcatcgattg attttttaca
     6421 aatttatgac tattgggcat aatttgatat tcaaagcaca gttacttgct tcagatcatg
     6481 gttgcctgga tgatttgccc ggtcaaatca ttgaaataaa ccgctatgaa cattcatgag
     6541 gaaacccgcc agttcatcat ataaaacctc cggatcaacc gccatccccg ttttgatctt
     6601 tacaacagtc ctgtttttat caatcagcaa ccttgtcggg ctgccgaata cattcatttt
     6661 ccgggcagcc tcttcctcca tcacgcaaag cacagggtac aaaggcttta agcctgcaag
     6721 ataagattta aaagctgcag gatcatcaat atccgccttt gttctcacga ccaggcaaat
     6781 cacatcaaag tcatcatact gcttctttaa ccgggttata atcttcggga ggtcctccat
     6841 ggtacagcct ttcccgattc cgcaatgagc atcgcttacg gcaataatgg tttttcgcct
     6901 gatcatatcc ggcaggttaa accggttacc aggcaaatcc ataacctgca catccggtag
     6961 ttgtttcccg gctgtattct tttgcacctc ttcttgcagg tcagccatca gtttgtttac
     7021 gtcttgctgg gtgagtgcac tgcctgagtt catggctttc tcaaatgtcc cgaaatcgac
     7081 gggcataatg gtcccgggct tcctgctgca tgacagtgcc aggaaaacag cggcaagtat
     7141 taacaaatat ctcataaccc gatcagtaaa agaattttcg acccggttat ccgccaagtc
     7201 agctttatac agtcaaaggt aattcaacga atgtaatttg agtcctttta cctggtaacc
     7261 aaagataaaa tttgcggctc agaatctgat atactttcat gacaaagaat aatgtacacc
     7321 gccgttctct gtattaaact tacacacttt gttggacgtc ccccatgtca gaattgcaga
     7381 ggtatgttta agacggcacg ggacaggtgt tatatggcac gggacaggtt taatactgca
     7441 cgggacaggt tttatatggc acgggacagg tttaatactg cacgggacag gttttatact
     7501 gcgcaggaca gattttgtaa tgtacgggac aggtttaata ctgtacggga caggtttaat
     7561 actgcacggg ataggtgttg tactgcacgg gacaggctta agactactca ggataagttt
     7621 aagactacac gggataggtt taatactgta ttggacaggt tctatactgc acgggacagg
     7681 ttctatactg cacgggacag gttttatact gcacgggaca ggttttatac tgcacggatt
     7741 gatttacagt gttttagaca aataaatgag cttatgagta tcctgaatta tttgtaatta
     7801 gttcagtgtg ttgatgaaaa tttaaaaacc ggcaggatta gttctcaaca acaagttgtt
     7861 aattcataac atctatgtta cctgttaagg atggaaagta taacaaactg cccctggctt
     7921 tcggtaatca tttactcttt aaacggtatt taaattgcct gtaaacacca tttaaagggc
     7981 atttgaaaga tggctctcct tttactgaaa agtgcaggat tgttaaatct tgcagctcct
     8041 gtgttaaacg atttaaatca cgttcaaatt tcaaaggttg agcgcttaag gtgtcaaaaa
     8101 cgggtatata ccctttatat ttgccacgcc aatattttat tggcagtatt gaaaatacca
     8161 ctcaacagtg aatggacctg gtttcttaaa aagattagcg ttgcagcggc aggtttactt
     8221 tgtgccggca ggcaatgtaa tgggcccttt cagaaatctg gccgctataa ttaacacaat
     8281 ccgcgaaaag ttgcgcgcct ttaaagacca gcagaggcag tttgcaatgg tggtcagtgt
     8341 gctggtcgat ttcgggtatt ctttttctac cgtccggttg ttttatacaa gatacagtga
     8401 gagcctctcc agcgaatgca cacctgccct catcaggcgt tacttccgtg aatatctgga
     8461 gcttgtaaac agtgagcgca acaacttttc gcattacggg aagtataatt tcgggaaagg
     8521 aaacctttct gcggttcgcg acatgtcaga actcatcgag tttgacattt atatgcagtc
     8581 cctgtcagga tatgagggta ttggcatact gaatgacgca tatgagcttt cgccccgcca
     8641 aatatctttt ttagaatctg tctcctgtat aaacaactgt ttaagttacg ttgaattact
     8701 tttcctgctg gtttaccttg agatagccag cctgtattta catttactgg cgcttgccgc
     8761 aaaactcagg aaatacaggt tttcattaag caggcagtta tcatttgcca tgatcaccgg
     8821 caggccctcg cttgccaatt ctcctccttt catgccgggg atgtgaggct atttcagcag
     8881 tattttgtgt ttagtgtcaa tgctgcggct cctctgttat cattttctgt tactgcaagt
     8941 cagggtttaa gtcagggttt cagagccgtc agcagctact cacagggcac accgggttca
     9001 atccggctaa tgcatcagcc tctccgccgc ggatcatcat tttcccgtta gcactccggc
     9061 attgcccggc agggatggat ggttcacatc aaccagccat aagcgttcac ttctgaggaa
     9121 atatgccacc ccgggccggt ggcatgtacc cgcagagttc gcctggcaac acggccactt
     9181 ccccaccgta ccgtaaaaag ccacctgggg ttggcaataa ctcccgagca tcaggccctt
     9241 tactaacact aatcccctat gaccatgaaa cgattagttt catcaaggaa caagttcata
     9301 accttacaga tgattatgaa cgaaaatcag gatgcatttg aaggttttac taacgctata
     9361 gcatcacgcg ataaattcat ttcaaatacc gaacgcattt cggaactgct gtcactggtt
     9421 gacaggccgt acaccgaaat ataccacgag cgcaaggatg ccagcgacag gcttgtgtca
     9481 gcgcttgacg acgccattgg catcgcaatt tcagtggcag cgaaaatcga taatgcagtg
     9541 gcagtcaggg ctatgcagag ttatcgcatg tccatcaaag ctgtttcttt caatggaata
     9601 agcgagattt cactgagagt cattgatttt atcactgcaa atgctgaaga attccaggct
     9661 tcaggtttct ctgccgaaca gttaagtgtc ttgcagcagt tatcaggtgc tctccgggat
     9721 atattgcaat caacttcttt tgagttgagc acccgtaaaa cggccaaaac ggaactttca
     9781 tctctggcca gtgagaacag gatgatcctt aaggttgatc tggacaattt tgtcaaaagc
     9841 cgccgtaagt tcttccctgc actctacaat gcctacatga ccgaacgcag gatcaagcgc
     9901 cacaaacgca gatcatcggg tgaaaccaca ctctgcgaaa tctccggcac tattaccgac
     9961 agcgcaaccg gaatgccatt ggctaatgct gttgtcaatc ttctctcgcc cgaaaccatc
    10021 gaaacaaccg atgaagacgg cgtatatg
//
LOCUS       DAOPYB010000110         9977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_7927_length_9977_cov_10.486696, whole genome shotgun sequence.
ACCESSION   DAOPYB010000110 DAOPYB010000000
VERSION     DAOPYB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9977
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7927_length_9977_cov_10.486696"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKH58_08875"
     CDS             <1..281
                     /locus_tag="PKH58_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNX89182.1"
                     /translation="ELDPLLHSELRLAVMSILLSVAEADFVYIKESTKATAGNLSVQI
                     DKLCEAGYIQVERGFQGKRTRTVCKITTKGVEAFNNYVEALKGYLNIN"
     gene            374..2956
                     /locus_tag="PKH58_08880"
     CDS             374..2956
                     /locus_tag="PKH58_08880"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX89183.1"
                     /translation="MKNRIQGLTKIRFICFCCLFVLLPLVLSAQNQRLTVNFSDLQSN
                     DGRILVSLYKGKADYSAHKPFKSTSLTIDNGSATWMPDSISQGEYILSAFHDENVNGK
                     LDVSDSGMPEEGFAFSNNAQPKMGPPNPQEMLFSIKENENATQTIHMIYFGIKPKELE
                     TIEVVGEKKQFVNADADKTTYQVKDNTALSTGSMRDAVRKLPGVVISPSGDLNLNGKN
                     VSIYVDGIPSNLSGSDLKNYMDGLPANTIEKIELIENPGASYEAKANGGIINIITRNN
                     APDNFGGTLNLNYGNSLNHKFSPSLMLNGRNKKINWQLQSGYNWHQQDQFTDIKQTFT
                     TFTPNVIFTNNSTQKNLNRNFYIRPMMNFRLTENSYIVFNYNFNTYNNQHTSNIIRES
                     RNLTPAIKYSSVFGNPEANRNNEFVLKYKTMLDTLGRSLQFTGYYSNYNKIANAQSTQ
                     NLSTPLYGISTNNLNLNHAYGKVDLELPFKIVRLTAGAKYNITNAHNLGKYNFNNLMS
                     TVFENPVYNQETDFIYHEQNIAGYVEARKKMGNLSATLGVRFEDLLYESEVASADSVV
                     GGRITKLFPTLNLLYTVIPNINFSGKYSRKIAMPAYDQLDPNVNGYFDSYSKSAGNQN
                     LKPNFYDNYSLSVTAFNYATLGSYLSYSKNISLLSTTVQPHSFVSEKTSNDFSNIKMY
                     GLYISLPVPFGLITKGAEFFKQPTNMSNMSYVHFYVSCNFNQIKGLDNPGTMKPLWMY
                     NINTHIGLPHHFTLDANWFHMFKGNFQIYSFGKPMNYWQVDLSRKFLKEKLDLTAEVT
                     QMTAQFVKFSVPNVNTDFISRTDGVTFWVKASYRFGGFKSKEETQIEVEKKTLEGGEI
                     NVRK"
     gene            complement(3011..4459)
                     /gene="pyk"
                     /locus_tag="PKH58_08885"
     CDS             complement(3011..4459)
                     /gene="pyk"
                     /locus_tag="PKH58_08885"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNX89184.1"
                     /translation="MEQKKFTKIVATISDKRCDVDFIRQLYEEGMNVVRMNSAHLQRE
                     GFLKIINNVRAVSQRIAILIDTKGPEVRTTVAENNSIELKTGDIVKIIGDTDTVSTKE
                     CIAVSYPNFVRDLHIGDDILIDDGEIDLKVVSKDEKFLTCIVENDGSLGSRKSINVPG
                     VRINLPSLTEKDRQNILFAIEQNIDFIAHSFVRSKQDLLDIQEILDAHNSPIKIISKI
                     ENQEGVDNIDEIIQHCYGVMVARGDLGIEVPQERIPGIQRKLIRKCVKAHKPVIVATQ
                     MLHSMIENPRPTRAEVNDVANAIYYRTDALMLSGETAYGKYPIESVRTMSQIALEAEN
                     TKMPENDIPIEFGKGNVTAYLAHAAAEAGKNIDIKAIITDTFSGRTARYLASYRSSDP
                     ILAFCYNERASRELALSYGVYSYYKEELVEDERNYFTTLLQNIIGRGIISREDIICYL
                     SGPTSGSYGTTFLEINTANTLLSEIDNYTLPK"
     gene            complement(4518..4934)
                     /gene="aroQ"
                     /locus_tag="PKH58_08890"
     CDS             complement(4518..4934)
                     /gene="aroQ"
                     /locus_tag="PKH58_08890"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNX89185.1"
                     /translation="MKKIIIINGPNLNLLGKREPTIYGSAGFEEYFSELKNMFSDMDL
                     SYYQTNSEGLIIDKLHEAGFTFDGIVLNAGAYTHTSLAIADAVRAISTPVVELHISNV
                     FKREEYRHHSFLSEACRGCIVGFGLDSYKLAVESFR"
     gene            5118..5585
                     /locus_tag="PKH58_08895"
     CDS             5118..5585
                     /locus_tag="PKH58_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89186.1"
                     /translation="MDKQKLLLYKLKPGVSKQLLFFIAALIWTFAGVMLLFKGYQLLS
                     SNPENIWIKLTISIICGVLFYVFIFSGISYKHSTRIKLLVSEKPCAFSFFNWKSYLMM
                     SLMITMGIGLRISGIVSAGILSILYITMGIPLLTSALRFYSDGYNFNNTINKK"
     gene            5592..6179
                     /locus_tag="PKH58_08900"
     CDS             5592..6179
                     /locus_tag="PKH58_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89187.1"
                     /translation="MQFPLNFTFKITSIANDFRVNDATGAEVAFVRQKLFKLKEEIQV
                     FNNESRSELNFTIKANKWLDFSASYVFFDRQGREIGRIVRRGWKSLWKSTYDIYDENQ
                     QQDFSVHEENPFVKVFDGIFGDLPIIGMFTGYFFNPTYLVTRPDGTAIARLSKEKSFF
                     GRRFSVNKLTEFESGEENRILLGLMMMILLERQRG"
     gene            6221..7285
                     /gene="corA"
                     /locus_tag="PKH58_08905"
     CDS             6221..7285
                     /gene="corA"
                     /locus_tag="PKH58_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNX89188.1"
                     /translation="MSRTTRKRKEDIGLSPYEIKFRGQKKTEKTTMELFDFNLDYVHE
                     FSLKSTDKIKSCMTGDSISWLNINGLDEVNLMKELGVIFSIDNNIMSDILNPSVRPKV
                     QEFEHGIFVTLKMLQMNERTNKLSVENLSLIITENFLISFQEEKGDFFDPVRERIRKH
                     KNKIRLAGPDYLAFALLDMVVDNYIYIIGLLGDKIENLEERMTGNVNKQLPELINLYK
                     QELNFLRHHIKPTKEMILTLAKLESEFIHNENRVNYKELQDNMNEANELTDSYREMLY
                     DLLNIYHTAATTRLNDIMKVLTMISVIFIPLTFIVGVYGTNFEYLPEIKWHYGYYIMW
                     GIMVIVAILMIWFFRRKKWY"
     gene            7396..8337
                     /locus_tag="PKH58_08910"
     CDS             7396..8337
                     /locus_tag="PKH58_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNX89189.1"
                     /translation="MPNKPTTPNNTGEFHLTDISLSEIVSFIDWRFFFHAWRLTGKYD
                     GIETVCDCASCKTGWLQSFDEKDRPKAEEALKLFRDAREMLRQFQEDKTIKINAAYSI
                     NHAFAANDDIIIQTENQTIKIPTLRQQQPSSDGFCYCLADFLSEKDDYVGTFVTTVTG
                     AEELAAIFEKGDDMYQSILVKTLSDRLAEASAEWLHYKIRKEYWGYTPDENLTMEDML
                     KTKYSGIRPAIGYPSLPDQSIIFELEPLLHFSRVGVKLTENGAMYPNASVCGLYFAHP
                     LSKYFMIGKIDENQLADYARRRGKTTTEMRKWLAANI"
     gene            8525..9634
                     /locus_tag="PKH58_08915"
     CDS             8525..9634
                     /locus_tag="PKH58_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX89190.1"
                     /translation="MKYGFLIFFFAFILSMIVYTIVRGCQVLAPYGYSKTVYLVLMIV
                     LTTAMFGGLFFENQLPHSFARMLSFVGYSYFIVLAYLFTSFLLVDDVRIINHFFPFIG
                     NIKILRLSAMLLTIGAVGVMMIYGNIKFNRPEVVNLSIKSNKPVQHKEIRIVAISDIH
                     LGVSIDKKRLQQYVTMVNAQNPDLVLIAGDLVDRSLKPVIAQNMDEELRAIKAPLGVY
                     GIFGNHEYYGESDHHISDFYRKSNIRLLRDTCVLVNNEFYLAGRDDKHNPHRKNLEDI
                     LISHSDKPVILLDHQPYHLEDAERNNVDLQISGHTHNGQFFPANLIVKSIFELGYGYL
                     KKGNTNYYVSSGLGLWGPQFRVASQSELVVINFNY"
BASE COUNT         3165 a         2049 c         1926 g         2837 t
ORIGIN      
        1 aggaactcga tccgctgcta cattctgaac tccggctggc cgtcatgtcc attcttcttt
       61 cggtagcaga agccgatttc gtttacatca aagaatcgac aaaagccacg gctggaaatc
      121 tgagtgttca gattgataaa ttgtgcgagg caggttatat tcaagtcgag cggggatttc
      181 agggaaagcg cactcgtact gtttgcaaaa ttaccactaa aggtgtggaa gcttttaata
      241 attatgtgga agcgctgaaa ggttatttaa atattaactg acacacaatt caggtgtctg
      301 tatagctgtt tttttagcaa tcatcggtat tattatccaa aaaattatcc caaaaaccaa
      361 attcaccaaa aaaatgaaaa atcgaattca gggattaaca aagatcagat ttatttgttt
      421 ctgctgcctg tttgttttac tgcccttagt tctttcggca caaaatcaac gacttaccgt
      481 aaatttcagc gatttgcaga gcaacgacgg ccgaatttta gtcagtcttt acaaagggaa
      541 agcggattat tctgctcata aaccatttaa atccacctcg ctcaccatcg ataacggatc
      601 agccacctgg atgcctgaca gcatttcgca gggagaatac attctatcgg cattccatga
      661 tgaaaatgta aacgggaaac tggatgtatc ggacagcggg atgcctgaag aaggatttgc
      721 attctccaat aacgcacaac ccaaaatggg acctcctaac cctcaggaaa tgctttttag
      781 cattaaagaa aacgagaatg cgacacagac cattcatatg atttatttcg gtatcaaacc
      841 aaaggaactg gaaacaattg aagtggttgg tgaaaaaaaa caatttgtaa atgcagatgc
      901 cgacaagaca acgtatcagg ttaaagacaa tacagcactc tcaactggct ccatgcgcga
      961 tgccgtaaga aaactgccgg gagtggtaat aagtccttca ggtgatttga atctaaacgg
     1021 aaaaaacgta agcatatacg tggatggcat tcctagcaac ctttcgggat cggatttgaa
     1081 aaattatatg gatggtttgc ccgcaaatac gattgaaaaa atagaactga ttgaaaatcc
     1141 gggcgcatcc tacgaagcta aagcgaatgg cggaattatc aatattatca cacgcaataa
     1201 tgcacccgat aacttcggtg gtacgctcaa cctgaattac ggaaacagcc tgaatcacaa
     1261 attttcacct tcgctgatgc tgaacggacg aaataaaaaa ataaactggc aattgcaatc
     1321 gggttataac tggcatcagc aagaccagtt tacagacata aaacaaacat ttacaacatt
     1381 tacacctaac gtaattttca ccaataattc cacgcagaag aaccttaacc gtaattttta
     1441 tatccgcccg atgatgaact ttcggctaac tgagaattca tacattgtgt tcaattacaa
     1501 tttcaacacc tataacaatc agcacacgag caatatcatc cgcgaaagcc ggaaccttac
     1561 tccggctata aaatattcat ccgtattcgg caatccggaa gcaaaccgca acaacgaatt
     1621 tgttctgaaa tacaaaacca tgctggacac gctgggtcgt tcgctgcaat ttaccggcta
     1681 ttattcaaac tataacaaaa ttgccaatgc gcaatccact caaaatttat ccactccgct
     1741 gtatggtatc agtactaaca atctgaacct gaaccatgcc tacggaaaag tggatttgga
     1801 acttccattc aaaatagtaa ggctgacagc cggtgcaaaa tacaacatca cgaatgctca
     1861 caatttaggt aaatacaatt tcaacaatct tatgtccact gtcttcgaaa atcctgttta
     1921 caatcaagaa accgacttta tctaccacga acaaaatata gccggttatg tggaagctcg
     1981 aaaaaaaatg gggaatctgt ctgctacact cggtgtgagg tttgaagacc tgctttatga
     2041 aagcgaagta gcgagcgccg acagcgttgt gggaggtaga attactaaac tttttcctac
     2101 tttgaatctg ttatacacag ttattccaaa catcaatttt tcgggaaaat attcccgaaa
     2161 aattgcgatg cctgcttacg atcagctcga cccgaacgtg aacggttact tcgacagtta
     2221 cagcaaatca gccggaaatc aaaacctgaa acccaacttc tatgataatt attcgctgag
     2281 tgtcaccgct ttcaattatg ccaccctagg aagttacctg agttactcaa aaaacatcag
     2341 tttgctttcc accactgtgc aaccacattc tttcgtatcc gaaaaaacat caaacgactt
     2401 cagcaatatt aaaatgtacg gcttgtacat atcgcttccg gttcctttcg gactgataac
     2461 taaaggggcg gaatttttca aacaaccgac aaatatgtca aatatgagtt atgttcattt
     2521 ttatgtttcc tgtaatttca accagataaa agggctggat aaccccggca ctatgaaacc
     2581 gctctggatg tacaacatca acacacatat tggtttacct caccatttca cgctcgatgc
     2641 aaattggttt cacatgttca agggcaattt tcaaatctac tcattcggga agccaatgaa
     2701 ttactggcag gtggatttat cgcgaaaatt cctcaaagaa aagcttgatc taactgccga
     2761 agtaacacaa atgacagcac agtttgtcaa attcagtgta ccgaatgtca ataccgactt
     2821 cataagcaga acagatggtg tcactttctg ggtaaaagca tcgtaccgtt tcggtggttt
     2881 taaaagcaaa gaggagacac aaattgaagt tgagaaaaaa acactggaag gtggcgaaat
     2941 aaacgtgagg aaatagacag aaacataaaa cgacagagac acccggtatg gatgtctctg
     3001 tttgatttta ttattttggc aacgtgtaat tatctatttc agaaagtaac gtatttgctg
     3061 tattaatctc gaggaaggtg gtaccatagc tgccgcttgt gggtccgcta aggtagcaga
     3121 taatatcttc ccgggaaata atgccacgac caatgatatt ttgcagcagt gtggtaaaat
     3181 aatttctttc atcttccacc agttcttcct tgtaatagga atacactccg taagataatg
     3241 ccagctctct ggaagcacgt tcgttgtaac aaaaagcaag gataggatcc gaactgcggt
     3301 aagaagccag atatcgggct gttcggccac tgaaagtatc ggtaatgatg gctttgatat
     3361 caatattttt tccggcttcg gctgcagcat gtgccagata tgcagttaca ttgcctttac
     3421 caaattcaat cggaatatca ttttcgggca tttttgtatt ttcagcctcc aacgcaattt
     3481 gagacatagt ccgaactgat tcgatcggat attttccata agcagtttcg ccgctgagca
     3541 tcaacgcatc ggtacggtaa taaatggcat ttgccacatc attgacttcg gcacgtgtgg
     3601 gacgcggatt ttcaatcatg gagtgaagca tttgagtggc gacaatgacc ggtttatggg
     3661 ctttcacaca tttacgaatg agtttgcgct gtattcccgg aattcgttcc tgcggaactt
     3721 caattcccaa atcgccacgg gcaaccatca ccccataaca atgttgtata atttcatcaa
     3781 tattatcgac accttcctga ttctcgattt ttgaaataat ctttatcggg ctgttgtgtg
     3841 catcgagaat ttcctgaata tcgagcaggt cctgcttgga tcggacaaag gaatgagcaa
     3901 taaaatcaat attctgttcg atagcgaaca gtatattctg acggtctttc tccgttagcg
     3961 agggaagatt gatacggacg cccggtacat tgatactctt acggctaccc aatgaaccat
     4021 cattttcgac tatacaggta aggaattttt catccttcga gaccactttc aaatcaattt
     4081 ccccgtcatc aatcaggata tcgtctccga tatgcaaatc gcgaacaaaa ttcggataac
     4141 ttaccgcaat acattctttg gtggaaacag tatcagtatc accaataatt tttactatat
     4201 caccggtttt aagttcgata gaattatttt cggcaacagt agtacgcact tcaggtcctt
     4261 tggtatcaat cagtattgca attcgttggc tgacagccct cacattattg ataattttca
     4321 gaaagccttc acgctgaaga tgagccgaat tcattctgac cacattcata ccttcttcat
     4381 aaagctgacg gataaaatcc acatcgcagc gcttatcact gatggtggct actatttttg
     4441 taaacttctt ttgttccatg attaactaaa cttgtttcgt tatttttcta tagttcttaa
     4501 tatcatatcc tgatttttta tcgaaaactt tccaccgcaa gcttgtagga gtcaagccca
     4561 aacccgacaa tacaaccccg gcatgcttcc gataaaaaag aatgatgacg atattcttca
     4621 cgtttaaata cattggaaat atgaagttca accacaggcg tgctgatagc ccgaactgca
     4681 tcggcaatag ccagcgacgt atgggtatat gcgccggcat tgagtacaat accatcaaag
     4741 gtgaaacctg cttcatgcag tttatcaatg atcagccctt cggaatttgt ctgataatag
     4801 cttaaatcca tatcagaaaa catatttttc aactctgaaa aatattcctc aaagcccgct
     4861 gaaccatata tggtgggttc tcttttccca agcaaattga ggttgggacc attaataatg
     4921 attatttttt tcatccgttt atgtatttat ttccgcaaaa ttacaaaaaa taatcagcgc
     4981 atgaaactac agacgggtgc gttaattatt ttatacagat gataaacaaa taatatgttt
     5041 aagagtttat ctgtctaaac cagttttctt caaaaaatat atcaaaacta aaattattct
     5101 gtatttttga caaaattatg gataaacaaa agttgttatt atataaattg aaaccgggag
     5161 tatcaaaaca gttgctgttt tttattgccg ccctgatctg gactttcgcc ggtgtaatgc
     5221 tccttttcaa aggttatcag ttgctttcgt caaatcctga gaatatttgg attaagttga
     5281 caattagcat tatctgcgga gttttatttt atgtgtttat tttttccgga atttcgtata
     5341 aacattcaac gcgcattaaa ttgcttgtat ctgaaaaacc ctgtgctttc tcatttttca
     5401 actggaagag ttacctgatg atgagcctga tgattacgat gggtatcgga ttacgaataa
     5461 gcggtattgt ttcagccggg atattgtcaa ttttatatat tacaatgggc attccactac
     5521 ttacttcagc actgcgtttc tattccgatg gttataattt caacaacaca ataaataaaa
     5581 aataaacttc tatgcaattc ccgttaaatt tcacatttaa gattacctcc attgccaatg
     5641 attttcgtgt taatgatgct accggtgccg aagtagcgtt tgtacgccag aaattattca
     5701 aactgaagga agaaatacag gttttcaata acgaaagccg ctcagaactt aattttacca
     5761 taaaagcaaa taaatggctt gatttttcag cctcgtatgt atttttcgac agacagggac
     5821 gtgaaattgg ccggattgtg cgccgtggct ggaaatcatt gtggaaatcg acttatgata
     5881 tatacgacga aaaccaacaa caggatttca gtgttcatga agaaaatccg tttgtcaaag
     5941 tatttgacgg aatcttcggc gatttaccca taatcggtat gtttacaggc tatttcttta
     6001 atccaaccta tctggttacc cgacccgatg gaactgccat agcccgttta tcaaaagaaa
     6061 aatcattttt cggacgtcgg ttcagcgtaa acaaactgac cgaattcgag tcaggtgaag
     6121 aaaatcgcat tttgctgggt ctgatgatga tgatactgct ggaacgacaa cgcggttaat
     6181 tttcttaaaa ataaaatatc gaccacttag aaacattatc atgtcacgca caacaagaaa
     6241 aagaaaggaa gacatcggtt tatcgcctta cgaaataaaa tttcgcggac agaaaaaaac
     6301 tgaaaaaacc acgatggagc tttttgactt caacctggat tatgtgcacg aattctccct
     6361 gaaatctacc gataaaataa agtcatgcat gacaggtgac agcatctcat ggctcaatat
     6421 taacgggttg gacgaagtaa atttaatgaa agaacttggt gtaatcttca gtatcgacaa
     6481 taatattatg tccgacatcc tcaatccatc agtaagacca aaagtacagg aatttgagca
     6541 cggaatcttt gtcacactga agatgcttca aatgaatgaa cgaaccaaca aattgtcggt
     6601 agaaaatctc agtctaataa ttacagagaa ctttttgata tcgtttcagg aagaaaaggg
     6661 tgatttcttt gatcctgtca gagagcggat acgcaagcat aagaacaaaa ttcgcttggc
     6721 aggacccgat tacctggcat ttgcactact tgacatggtg gtggacaact acatatatat
     6781 catcgggctg ttgggtgata agattgaaaa cctggaagaa agaatgaccg gcaacgtgaa
     6841 caaacaactt ccggaactta tcaatcttta caagcaggaa ctcaacttcc tgcgacacca
     6901 tattaaacca accaaagaaa tgatactcac tctggccaaa ttggaatcgg aatttatcca
     6961 caatgaaaac agagtgaact acaaggaact tcaggacaat atgaacgagg ccaatgaact
     7021 gaccgacagc taccgagaaa tgctctacga cctgctgaac atttatcata ccgccgcaac
     7081 aacaagactg aacgatatca tgaaagtgct gacaatgata tcggttattt ttattcccct
     7141 cacttttatt gttggcgttt atggaaccaa ttttgaatat cttcccgaaa taaaatggca
     7201 ctacggatac tatatcatgt ggggaataat ggtcatagtt gcaattctga tgatttggtt
     7261 tttccgaaga aaaaagtggt actgatgaac ttcgacacat tctgattgtt actttcatgc
     7321 aggctgaata agcctgtatt ttttaattat taccgtatat gatttcatat acaatattaa
     7381 ttacgctaat agctaatgcc aaacaaaccc acaactccaa acaatacagg tgagttccac
     7441 cttacagata taagtttatc tgaaattgtt tcattcatcg actggcggtt cttttttcat
     7501 gcctggcgac tcaccggtaa atatgacgga attgaaacag tttgtgattg cgcatcgtgc
     7561 aaaacgggct ggttacaatc ttttgacgag aaagaccgcc caaaagcaga agaagcactg
     7621 aaactcttca gagatgcacg ggaaatgctc cgacaattcc aggaagataa aaccattaaa
     7681 atcaatgctg cctactccat taatcatgca ttcgctgcca acgatgatat tatcatacag
     7741 acagaaaatc aaacgataaa aataccaacc ttacgccagc aacaaccctc ttccgatgga
     7801 ttctgctact gccttgccga ttttctgagc gaaaaagatg attatgtcgg aacctttgta
     7861 accacagtta ccggagcaga agagcttgct gccatatttg agaaggggga tgacatgtac
     7921 cagtctattc tggtgaaaac attatccgac cggctggctg aggcatcggc agaatggctt
     7981 cactacaaga ttcgaaaaga gtattggggt tataccccag acgaaaacct gaccatggaa
     8041 gatatgctga aaacaaaata tagcggcatt cgtcctgcca taggatatcc ttcgttgccc
     8101 gatcagtcca tcatttttga actggaacct ctgctgcatt tcagccgcgt tggagtgaaa
     8161 cttacagaga atggcgccat gtatcccaat gcttcggtat gcggattgta ttttgcccat
     8221 ccgctgtcga agtattttat gatcggtaaa attgatgaaa accagctggc tgattatgcg
     8281 cgaaggcgcg ggaaaaccac caccgaaatg cggaaatggc tggcagcgaa tatttaaggt
     8341 cccttaaatc ccccaaaggt aaaagccccc taaatccctc ccgaaacaag ttcgggacag
     8401 gctctcaagg gggacttaaa gaaaaagata gtttcttcta attcttttca atttgaatat
     8461 ttatctgaaa tatgaagtct gaagttccat tttcgaaatc taaaaaaata aaaatgatat
     8521 atttatgaaa tacggttttc ttatattttt ctttgcgttt attctgtcca tgattgttta
     8581 taccatagtc agaggttgtc aggttctggc tccatacggt tattccaaaa cagtgtattt
     8641 ggtattgatg attgtgttga caacggcgat gtttggagga ttatttttcg aaaatcaatt
     8701 accacatagt tttgcccgga tgctgagttt tgttggatac agttatttta tcgtgctggc
     8761 atatttgttc acttctttcc tgctggtgga tgacgtccgg attatcaatc acttttttcc
     8821 atttattggt aacataaaga ttttacggtt atcagcaatg ttgctgacaa ttggggcagt
     8881 tggcgtaatg atgatttacg gaaacattaa gttcaacagg cctgaagttg tcaacttatc
     8941 cataaagagc aataaaccgg ttcagcacaa ggaaataaga attgtagcca tcagcgatat
     9001 ccacctgggt gtatccatcg acaaaaaaag acttcaacag tatgtaacaa tggtcaatgc
     9061 ccaaaatccg gaccttgttc ttattgccgg tgatttggtg gatcgttctt tgaaaccggt
     9121 cattgctcag aatatggatg aggaactgcg tgcgataaaa gctccgctgg gtgtatacgg
     9181 tatttttggc aatcacgaat attatggtga aagcgatcat catatttcag atttctaccg
     9241 aaaatcgaat atcaggttgc tcagggacac ctgtgtgttg gttaataacg aattttatct
     9301 tgccggacgg gacgataaac acaatccaca ccgtaaaaat ctggaggata tactgatctc
     9361 ccattcagat aaacctgtca ttctcctcga tcatcagccc tatcatctcg aagatgctga
     9421 aagaaataac gtggatttgc agatttcagg acatacccac aacggacagt ttttccccgc
     9481 caacctgatt gtaaaaagta tattcgaact gggttatggt tacctgaaaa aaggaaacac
     9541 aaattactat gtttcatcgg gtctgggact ctggggacct caattcaggg tagccagcca
     9601 gagtgaactg gtagtgatta atttcaatta ttaaaaaacg ccccctagcc ccttaccgaa
     9661 acaagtcaga gacaggcctc atcctcccac acgtgagaac aaggggagca gaggaacttt
     9721 gagtcatatt aatatggcat cgtgtcatat cattatggcg atgagtcata ttaatatggc
     9781 aaactgccat aatggaatgg gtaaaacaca taaaatacag acgtttctgt tttattttac
     9841 cggacaacaa taattttatt ttcaataatg actgccttaa tatccgaagt acaaagcatc
     9901 cgataataaa gtgttcagaa aacgctcact ccagcaattt atccactctt acaaattcgt
     9961 aacctttgcc tttcaga
//
LOCUS       DAOPYB010000111         9870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_8042_length_9870_cov_9.222924, whole genome shotgun sequence.
ACCESSION   DAOPYB010000111 DAOPYB010000000
VERSION     DAOPYB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8042_length_9870_cov_9.222924"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKH58_08920"
     CDS             complement(<1..356)
                     /locus_tag="PKH58_08920"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNX89191.1"
                     /translation="MSALIKVNKNIIFKVFSLIYGAVTEIRNSFFDLGILKTKSFDLP
                     IISIGNLAVGGTGKTPHTEFLIQQVSKNFKIAVLSRGYKRETRGFVLADKNSTAKTIG
                     DEPYQLFGKFPAITVAV"
     gene            complement(496..2256)
                     /gene="sppA"
                     /locus_tag="PKH58_08925"
     CDS             complement(496..2256)
                     /gene="sppA"
                     /locus_tag="PKH58_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNX89192.1"
                     /translation="MKQFLKFTLATITGIIITSLLGFLLFFGMIGAIASSAEKTVKIE
                     PNSVYELDLEGQLVERSQDDPFSGFFSDAMGGYEVKNIGLDDVLANIKKAKTDDNIKG
                     IYLKGGTLLGGYASVTEIRNALIDFKKSGKFIVAYADTYTQKMYYLCSVADKVYLNPQ
                     GMLEFQGIAANTMFMKNTLDKLGIEAQVVKVGTFKSAVEPYINTKMSDANKEQVTTFI
                     SSIWNNVLNEISTSRKIPKDTLNRYADEMMSFQPAEKLQQYKLVDSLTYIDGMENVLK
                     KMLNTDKDKKLRTVSHSDMCNVPVSEKMQKEKIAVIYGVGGIDMNESDGIVSKDLVET
                     INKVSEDKSVKAVVFRVNSGGGSAYGSEQIWHALGGLKSKKPVIVSMGDYAASGGYYI
                     SCMADSIVAQLNTLTGSIGIFGVIPNINGLNNKIGLTYDGVKTNKLSDAISFNRAFTP
                     EERNIMQNYVNRGYEIFVKRCADGRKKTPEQIKSIAEGRVWTGEDAKKIGLVDKIGGL
                     NDAIEMAAKKAKLTAYQVKTYPEKENFMTRLMKDFNSTIETRIMKGQLGEKYEVLKQI
                     KQIERYNGIQAVMPFDVSMN"
     gene            complement(2353..2655)
                     /gene="trxA"
                     /locus_tag="PKH58_08930"
     CDS             complement(2353..2655)
                     /gene="trxA"
                     /locus_tag="PKH58_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX89193.1"
                     /translation="METFNEIINSDKPVLIDFYATWCGPCKTMHPIIENIGHEVQGKA
                     RVLKIDVDKNPSLAARYQIQAVPTLLIFKKGNVVWKHPGGLDKQSLLNTLLSHTAI"
     gene            complement(2750..3178)
                     /locus_tag="PKH58_08935"
     CDS             complement(2750..3178)
                     /locus_tag="PKH58_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNX89194.1"
                     /translation="MSLVRFSQTPWFDRFFDNDLLDWSNRNFSQTNTTLPSVNIKESD
                     EAFMVEVAAPGLDKKDFKIEVHNNLLTISSEKQEENEAKNGNYTKREFSYQSFCRSFT
                     LPATANGDNISAGYDKGILTVTIPKKEEAKPKPARMIEIN"
     gene            complement(3456..4178)
                     /locus_tag="PKH58_08940"
     CDS             complement(3456..4178)
                     /locus_tag="PKH58_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89195.1"
                     /translation="MKKTITLIILSIWFTFASAQSDEPSYKFSAGDKSIIITSGFSLP
                     SFDIGGKLYEKQLHDLSPTVNFGFRYLLPNNFALKLFGEYTTYKDDDIGTKNDTRGGG
                     GKYNFSNQIYRVGGRLEYNLFGNVSDFESRHNVYAFGGGGYAYATSIVNGDPDKDHFK
                     NTTPVNIPLLLTGIGYQYRFSKNFGLGLDLNYEYYFSDYMEGFHTTWSKSNDVAYSAM
                     LTVSYFFTKSYYHTKKCRCEKD"
     gene            complement(4298..5026)
                     /locus_tag="PKH58_08945"
     CDS             complement(4298..5026)
                     /locus_tag="PKH58_08945"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNX89196.1"
                     /translation="MKKLILISIFLLFNTSAFPQGKGLLSQIPFGEVSLFGGAGPNKM
                     SGDIGGDNLKSLLQNDMSYGFSLGARYTFKYNFALRVFGDLSNYTGKDKEVYNLYGQN
                     LRRLSFKTDVLSLGGQLEIILFGNSLAKNPIPHSVYLLAGLKTTKVDATLSNPNNNQN
                     GPDETKTPIAHFFGIGYQYRYSNLLSFGLEAKENYFHSDRMDGYNPVIPANKYRDAAF
                     DIKFTVAYYIPDRNRLNSYNRRWE"
     gene            complement(5152..6819)
                     /locus_tag="PKH58_08950"
     CDS             complement(5152..6819)
                     /locus_tag="PKH58_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNX89197.1"
                     /translation="MEKTVVIYCKNTQDYHSFPLGISLLEIYQSLNINLKYQVVAARV
                     NYKVEDLNFLIYKPKDIEFIDTSTSSGMRVYVRTLSMVLSKAVYELYPHAEIRIEHPI
                     SKGYYCRIEHLNEPLTTGIVRRIKDRMIEIIGRKEPIICEEQQAEHVKKLFRNGKNNT
                     TKDTLFESLGDPYLRYFRIGDFIDYYTSILLPSTDYLNIFDLIPFYDSMLLQVPDREN
                     PDRLEEMVLQPKMYEIFKEYLGWNRIMDLKSVGEFNRACQNNQAFNLIKIAEALHEKK
                     VASIADMIQQRPKVKVVMISGPSSSGKTTFSKRLSIQLLVSGLKPKSISLDNYFVDRE
                     HTPRDENGDWDFEALHALDLPFFNQQLNQLMNGEEISLPTYSFEDGKRYFKGEKLRLE
                     EDTILIMEGIHALNPELIQSISHDAIFKIYVSALTTISIDEHNWIPTADTRLLRRIIR
                     DYRYRGYSARDTISRWPSVRRGEEKWIFPYQENADVMFNSALIFELAVLKRHVEPILE
                     QVPKYCEEYTETHRLIKFLRYFVAIPDREIPPTSLLREFVGGSSFRY"
     gene            complement(6955..8250)
                     /locus_tag="PKH58_08955"
     CDS             complement(6955..8250)
                     /locus_tag="PKH58_08955"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX89198.1"
                     /translation="MKNSYFTKTNKYSLCMLIGLSILIFIQPVSAGVKNHLQKPDSLF
                     NSYKGLIVDINSREKLPYASLTVENTNISTVTNADGEFLLKVPKTDTNRMVVITFLGY
                     GSKKISLSALQPDGNKIELRSLPIFLPELEVVSKDPQFLIRKMFDKRTENYSNKDMIM
                     NAFYRETIRKRNTNVSLSEAIVEVYKQSYNNNLTDMAALYKSRKSTDYSKLDTLVYKL
                     MGGPFNSLYLDVMKYPDFIFTSDILNNYSFRFLQTDRIDERLIYVIGFEQLKHVIEPL
                     FYGKLYIDAESYALVRADFDMNLTDNDEATKLFIRKKPFNAVVNTTKAHYQIEYRLND
                     DKWYYAYSRVDLNMKINWKKKLFNTYYNSTIEMASTDWFEDVNKKGFKMKDRINPNVV
                     IQDAVTGFYDPVFWGEYNIIEPDKSIENAINKIQKKNKK"
     gene            complement(8318..9043)
                     /gene="recO"
                     /locus_tag="PKH58_08960"
     CDS             complement(8318..9043)
                     /gene="recO"
                     /locus_tag="PKH58_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNX89199.1"
                     /translation="MQVKTSGIILHQVRYTDSTVIVNIFTREFGRMAYFVRGANKKGS
                     ACRSALLQPLSIVEIEAVHHSRKTIQTIREMRVAVPFFDIPFDPVKNTLALFIAEILL
                     KTLKHSENDDDLYNFIENSIRELDTCKEGLGNFHLVFMIQLSHYLGFGPNIENAEEAH
                     FFDLMNGVFDIQAPSHTHILNAEYTSSFRTLMTLDYENMNQMKLNRAKRSELLDRLIE
                     YYQLHIPDFHPVTSIDILHKLWD"
     gene            9260..>9870
                     /locus_tag="PKH58_08965"
     CDS             9260..>9870
                     /locus_tag="PKH58_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNX89200.1"
                     /translation="MELASKYDPSEVEDKWYKYWLDNKFFHSEPDDREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLVRRARMQGKNACWVPGTDHASIATEAKVVNRLAAQGISK
                     ADLTRDEFLKHAWEWTHEHGGIILEQLKKLGASCDWDRTAFTMDQKRSDSVIKVFCNL
                     FDKGLIYRGVRMVNWDPKALTAISDEEVIHKEVNGKLYYLRYKV"
BASE COUNT         2861 a         1862 c         1890 g         3257 t
ORIGIN      
        1 acagctacgg tgattgccgg aaatttgccg aaaagctggt aaggttcatc acctatggtt
       61 tttgcagtgc tgtttttatc cgcaagaaca aatcccctcg tctcgcgttt gtatcctctg
      121 cttaaaacag caatcttgaa gtttttgctt acttgttgta ttaaaaattc agtgtgcgga
      181 gtttttccgg tgcctcccac agccagattt ccaatgctga taatcggtaa atcgaatgat
      241 tttgttttca aaattcccag gtcaaagaaa ctgttgcgga tttcggtaac agcgccgtaa
      301 ataagcgaaa aaactttgaa aataatattt ttgtttactt ttatcaatgc actcataatt
      361 cgttaataca aagatatgaa atgttttgga aattggctga ttgtttatat tacaatttgt
      421 aaaccattaa actgaataaa aaagcgtttt cggaattccg aaaacgcttt gatagaagat
      481 gtatatttct gaaatttaat tcatggaaac atcgaaaggc attacagcct gaattccgtt
      541 atatctttct atttgtttta tttgtttgag aacttcgtat ttttcaccca gctgaccctt
      601 catgattcgt gtttcgatgg ttgagttgaa atccttcatc agtcgggtca taaagttttc
      661 tttttcagga tatgttttta cctgataggc ggtgagttta gcttttttgg cagccatctc
      721 aatagcatcg tttaaaccgc cgattttatc caccaatccg attttctttg catcttcgcc
      781 tgtccataca cggccttcgg caatggattt tatttgttcg ggtgtttttt tacgtccgtc
      841 ggcgcagcgt tttacaaaga tttcgtaacc gcggtttacg taattttgca tgatattccg
      901 ttcctcgggt gtgaatgcgc ggttgaagga gatggcatca ctcagtttgt tggtttttac
      961 accatcgtaa gtcaaaccga tcttgttatt cagtccgttg atattcggta tcactccgaa
     1021 aataccgatt gaacctgtca gagtgttgag ctgtgcaaca atgctgtcag ccatgcacga
     1081 aatgtaatat cctcccgaag ccgcataatc gcccattgat acgatgaccg gttttttcga
     1141 tttgagtccg cccagtgcat gccagatttg ttctgaacca taagcgcttc cacctcctga
     1201 gtttaccctg aaaacaactg cttttacaga tttatcttct gatactttat tgatagtttc
     1261 aaccagatcc tttgatacaa tgccatcact ttcattcata tcaatgcctc caactccgta
     1321 aatgacagca attttttctt tttgcatttt ttcggacaca ggaacattgc acatatcaga
     1381 atggctgaca gttctcagtt ttttatcctt atctgtattc agcattttct tcagtacgtt
     1441 ttccataccg tcaatgtagg tcagtgaatc cacaagttta tattgttgca gtttttctgc
     1501 aggctgaaat gacatcatct cgtccgcata gcggttcagt gtgtctttcg gtatcttgcg
     1561 cgatgtcgat atttcgttca ggacattatt ccagatcgaa cttataaagg ttgtgacctg
     1621 ttccttattc gcgtcgctca tttttgtatt gatgtaaggt tccacagccg atttgaatgt
     1681 tccgactttt acaacctgag cttcgatgcc aagtttatcg agggtgtttt tcataaacat
     1741 cgtattggct gcaattccct gaaattcgag cattccctgt ggatttagat aaactttatc
     1801 ggctacgctg cacaggtagt acattttttg agtataagta tcagcatagg caacgatgaa
     1861 ttttccggac ttcttgaaat cgatcagggc gttgcgtatt tctgtaacag aagcgtatcc
     1921 tccgaggagc gtaccacctt tcaggtaaat acctttgatg ttgtcatctg tttttgcttt
     1981 ttttatattg gctagtacgt cgtccaaacc gatgttttta acttcataac cacccattgc
     2041 atccgaaaaa aatcctgaga acggatcgtc ctgtgaacgc tccaccagtt gaccttccag
     2101 atccagttca tataccgaat tgggttcaat cttaaccgtt ttttcggcgg agctggcaat
     2161 tgccccgatc ataccaaaaa ataacaggaa tcctaaaagt gatgtgatga taatacctgt
     2221 aatggttgct agtgtgaatt tcagaaattg tttcataagt ttaatgtttt caaatgatta
     2281 ttgttgcaaa gtaactaaat tttttgaata caataatgca ttttaatcaa atataaaaca
     2341 aatgactgct aattatatgg cagtatgaga aagtaacgta ttaagcaggc tttgtttatc
     2401 gagaccaccc ggatgtttcc agaccacatt tccttttttg aatataagca gtgtaggcac
     2461 tgcctgaatt tgataacgtg cggccagcga ggggttcttg tcgacatcaa ttttaagtac
     2521 ccgggctttt ccctgcacct catgtccaat attttctata attggatgca tggttttgca
     2581 cgggccacac caggttgcat aaaagtcaat taaaacaggc ttatcggaat ttattatttc
     2641 gttgaatgtt tccataagtt acgattatga attaatgaat tatgattgaa tgaatttaaa
     2701 aagaagaggg aaggtgagtt tatttacctt ccctcgggtg ttttgaaatt tagtttattt
     2761 caatcatgcg agccggtttg ggttttgctt cctctttttt cggaattgta actgtcagta
     2821 ttcccttgtc ataacctgcg ctgatattgt cgccgtttgc agtagctggc agggtgaatg
     2881 atcggcagaa tgactgatag ctgaattcgc gttttgtata gtttccattt ttagcttcat
     2941 tttcttcctg cttttcagaa gaaatagtca ataagttgtt gtgaacttcg attttgaaat
     3001 cttttttgtc cagaccggga gcggcaactt ccaccatgaa tgcttcgtcg ctttctttaa
     3061 tattgacgga tggcaatgtg gtattggttt gtgaaaaatt tctgtttgac cagtctaata
     3121 aatcgttgtc aaaaaagcgg tcaaaccacg gtgtttgtga aaatcttaca agtgacataa
     3181 ttaaatcctc catattttaa gttaaacatt aattatttgc tgtttgaaca gatgaataca
     3241 aataaaatgc cacagcaaaa acatgacaaa atggcagttt cgctatcttg tttactgata
     3301 gtgtaaaagt tacgtcttgt cttgtattta agtaaggatt tgtgtaatag ataagtatgt
     3361 gtatgttact tttaataaat cacaaaataa agaaactgac aaaatggctg tctctaataa
     3421 ggggagaaga ttttgaaaaa aggtcggagt tgagtttaat ccttttcaca acgacatttc
     3481 ttggtatgat aatagctttt tgtaaagaaa taggaaactg taagcatagc actatatgcc
     3541 acatcgttgc tttttgacca ggttgtatga aatccttcca tataatctga aaaataatac
     3601 tcataattta aatccagccc taaaccaaaa ttttttgaaa atcggtattg ataaccaatt
     3661 ccagtaagca aaagcggaat atttacaggt gtagtgtttt taaaatggtc tttatccgga
     3721 tctccattta ctatagaagt tgcataagca tagcctccgc ccccaaacgc gtatacattg
     3781 tgacgcgatt caaaatcact tacgttccca aataaattat attccagccg gccgccaact
     3841 ctgtaaattt gattcgaaaa attatattta cctcctccgc ctctggtatc gttttttgtc
     3901 ccgatatcat cgtctttata ggttgtatat tcgccgaata atttcagcgc aaagttgttc
     3961 ggtaaaagat atctgaagcc gaaattaacc gtaggtgaca aatcatggag ttgcttttca
     4021 taaagttttc ctccaatatc aaaagacgga agagagaaac cactagttat gatgatagat
     4081 ttatcacctg cagaaaattt gtaggaaggc tcgtcacttt gagcagaagc aaaagtgaac
     4141 cagatagata atattattaa agttatagtt tttttcatta aaccacaaaa acagcataac
     4201 aaaagagtgg aaaaattaaa tggtaacaaa tttacaaaaa aataattgaa ctaagcagat
     4261 aaaaatattt ttaatcttta tttattcagt tcagtcatta ttcccatctt ctgttgtatg
     4321 aattcagtct gttacggtcg gggatgtaat atgcaacggt aaatttgata tcaaaagcgg
     4381 catccctgta tttgttggct ggtataaccg gattataacc gtccattcta tcggaatgaa
     4441 aataattctc tttggcttcc agtccgaaac tcaagagatt agaataacgg tactggtatc
     4501 ctattccaaa aaaatgggct attggagttt ttgtttcatc cggtccattt tggttattat
     4561 tcggattcga tagtgtggca tccacttttg ttgttttcaa accagctaat agatataccg
     4621 aatgcgggat tgggtttttt gctagtgaat tgccaaacag tattatctcc agttgcccgc
     4681 ccagactgag cacatcagtc ttaaagctaa gacgtctcaa attctggccg tataaattat
     4741 atacttcttt atcttttcct gtgtaattgg acaagtcacc aaaaactctt aatgcgaagt
     4801 tatatttgaa tgtgtaacgg gctccgagtg agaagccgta gctcatatcg ttttgaagca
     4861 ggcttttcaa attatcacca cctatgtcgc ccgacatttt attgggtcct gcaccaccaa
     4921 acaacgaaac ttcgccaaaa ggaatttgag acagcaatcc tttaccctgt gggaaggcgg
     4981 atgtgttaaa caacagaaaa attgaaatta agattagttt tttcataagc agttaaatac
     5041 agggttttaa actactgaaa agtataacac ataatgaagc atttgtgttt ttatcataaa
     5101 agatgaatga aatccattat caaatcgatc ccttttcaga ttaattgacc actaatatcg
     5161 gaagctacta cctcctacaa attcgcgcaa aagagaagta ggaggaattt ccctatctgg
     5221 tattgcaacg aaataacgaa gaaattttat gagacggtgc gtttctgtgt attcttcaca
     5281 atatttgggc acttgttcca gaatgggttc tacatgtctt tttagtaccg ctaactcaaa
     5341 tatcagtgcc gagttaaaca tcacgtcagc attctcctga tatggaaaaa tccatttttc
     5401 ttctccgcgc ctcacacttg gccagcgcga gatggtatcc cgggcagagt aaccacgata
     5461 acggtaatcg cggataatgc gtctcagcaa gcgggtatca gcagttggta tccagttgtg
     5521 ttcgtcaatc gaaatggtag tgagagctga tacatagatt ttgaagatgg catcatgtga
     5581 tatggactgg ataagttccg gattgagagc atgtattcct tccataatta atatagtgtc
     5641 ttcttccagt cttaatttct cgcctttgaa gtatcttttt ccatcttcaa aactgtacgt
     5701 cgggagtgaa atttcctcac cattcatcag ttggttaagc tgttgattga aaaacggcag
     5761 gtcgagtgca tgaagcgctt cgaaatccca gtcgccattt tcgtcacgag gtgtatgctc
     5821 acggtccaca aaatagttgt ccagcgatat ggattttggc ttcagtccgc tcactaaaag
     5881 ctgaatggaa agccttttac tgaaggttgt tttacccgac gaggatggcc cggatatcat
     5941 cacaactttc actttgggtc gttgttgtat catatcagcg atggaggcaa cttttttctc
     6001 atggagagct tcagctattt ttatcaggtt gaatgcctga ttgttctgac aggcacgatt
     6061 gaattcgccc acagatttca ggtccatgat acgattccag cccagatatt ccttgaaaat
     6121 ttcatacatt tttggttgca gaaccatctc ttcaagcctg tccggatttt cacgatcggg
     6181 aacctgaagt aacatgctgt cataaaacgg aataaggtca aatatattca gataatcggt
     6241 ggatggaagc agaatgctgg tataatagtc aataaaatct cctatccgaa aataacgaag
     6301 gtaggggtcg cccaaacttt cgaacagcgt gtctttggtt gtattgtttt tcccgtttct
     6361 gaacagtttt tttacatgtt cagcttgttg ttcttcgcag atgatgggtt cttttctgcc
     6421 gattatttct atcattctat ccttgatgcg tctgactatt ccggtcgtaa gtggctcgtt
     6481 caggtgttca atgcggcagt agtaaccctt ggaaatagga tgttcgatac gaatttcggc
     6541 atgaggatag agctcgtata cggccttaga aagaaccatg ctcagcgtac ggacgtaaac
     6601 acgcattccg gaagatgtgc ttgtgtctat aaattcaatg tcctttggtt tgtatatcag
     6661 aaaattaaga tcttcaacct tatagttaac acgggcagca acaacctgat atttcaggtt
     6721 aatgtttagt gattgatata tttcgagcag tgatattccc agtggaaagg aatgataatc
     6781 ctgagtgttt ttacaataaa ttacaacagt tttttccata aaagaatgat tctaatttga
     6841 ttcggggtat gatgtttttt tgctgcatcc cgaatgaatg caacagcaaa aatcatcaca
     6901 aatatagtta taatattttt aaatcagata atcgtagaat agtgaaattc taaactattt
     6961 tttatttttt ttctgaattt tattgatggc attctcaatc gatttatcgg gttcgataat
     7021 gttgtattca ccccagaata ccggatcata aaaccctgtt acggcatcct gaataacaac
     7081 gtttggattt atccggtctt tcattttgaa tcccttttta tttacatctt caaaccagtc
     7141 ggtggaggcc atttctattg tagaattgta gtaggtgttg aataatttct ttttccagtt
     7201 tattttcatg ttgagatcta cccggctgta ggcataatac cacttgtcat cattaagtct
     7261 gtattctatc tgataatggg cttttgtagt atttaccaca gcgttgaaag gtttcttccg
     7321 gatgaaaagt ttggtagctt catcgttatc cgtcagattc atatcaaaat ctgcccgaac
     7381 cagagcatat gattcagcat caatataaag tttgccataa aacaagggct caataacatg
     7441 ttttaattgt tcaaatccaa ttacataaat cagcctttca tctatcctgt ctgtctgaag
     7501 aaaccggaaa ctatagttgt ttaatatatc ggaagtaaaa atgaaatcgg ggtatttcat
     7561 tacatccaga taaagcgaat taaacggacc tcccatgagc ttatatacca acgtatcaag
     7621 tttgctataa tcagtgcttt tcctcgattt atagagagct gccatatctg tcagattgtt
     7681 gttgtaagat tgcttgtaaa cttcaacaat ggcttccgaa agcgatacat ttgtatttct
     7741 ctttcgtatg gtttcccgat agaaagcgtt cattatcatg tctttattgc tgtaattctc
     7801 cgtacgcttg tcgaacattt tccggattaa aaactgagga tctttcgata caacctccag
     7861 ctccggtagg aaaataggca gagatcttaa ttcaatcttg ttaccgtcgg gttgtagtgc
     7921 tgatagtgaa atttttttag atccatatcc taaaaatgtt attacaacca ttctgttcgt
     7981 atctgttttc ggaactttta gcagaaattc tccatcagcg tttgtcactg tcgagatatt
     8041 tgtgttttca actgtcagtg aggcgtaagg taatttctcg cgggaattga tatctacgat
     8101 taatcctttg taggaattaa aaagcgagtc gggcttttgc agatggtttt tcactccggc
     8161 acttacgggt tggataaaaa ttaaaatact caggccaatg agcatgcaaa gtgagtattt
     8221 atttgttttg gtaaaataag aatttttcat tttaatcagg ttttattctt taactaaaac
     8281 aataatcaat ataaaatgtt tcggctatag gaaagtatta atcccataac ttgtgcaaaa
     8341 tgtcaatgga agtaaccggg tgaaaatcag gaatatgtaa ctggtaatat tcaatcagtc
     8401 tgtccaacag ttcagacctt tttgcccggt tgagtttcat ctggttcatg ttttcgtaat
     8461 caagtgtcat cagcgtcctg aaagagctgg tgtattctgc attcagaata tgtgtatgtg
     8521 acggagcctg aatatcaaaa acgccattca tcaaatcaaa aaaatgagct tcctctgcat
     8581 tctcaatatt tgggccaaac ccgaggtaat gggataattg tatcatgaat accagatgaa
     8641 agtttcccaa accttcttta catgtatcta actcccgaat agaattctca atgaaattat
     8701 ataaatcatc gtcgttttcc gaatgtttca gtgtttttag cagtatttca gctatgaata
     8761 gagccagcgt gttttttacc ggatcaaaag gaatgtcgaa aaaaggaact gcaactcgca
     8821 tttccctgat tgtctgaata gtttttcggg aatgatgaac agcttctatc tcaacaatag
     8881 acagtggctg gagtaaagcc gatctgcagg ctgaaccttt tttattcgca ccccttacaa
     8941 aatatgccat ccgcccgaat tcacgggtga atatgttaac aataacagtt gaatctgtgt
     9001 acctgacctg atgcagtatg atacccgaag ttttaacttg catatcacaa aaatatgaat
     9061 aatttcacaa aattattcaa ttatgacctg atttgtctgt ttaaccggag ctttttgact
     9121 caaaacttta ttaaaaacac atttttatct atctttgcac tttatttttc agaaaaaagt
     9181 atgataatat ataattgaat tccaatcagt ttgataattc aatttttaca caacaaatcg
     9241 taatttgtaa attaataata tggaacttgc aagcaaatat gatccttcag aagttgagga
     9301 taaatggtac aaatattggt tagataataa gttttttcat tccgaacccg atgaccgtga
     9361 accttatacc atcgtgatcc ctccgcctaa tgttacggga gtgctgcaca tgggtcatat
     9421 gcttaacaat accattcagg atgtattggt acgtcgcgcc cgcatgcagg gaaaaaacgc
     9481 ctgttgggtt cccggaaccg atcacgcgtc aatagccacg gaagcaaaag ttgtgaaccg
     9541 gcttgcagct cagggtatca gtaaagctga tcttacccgc gatgaatttc tcaaacacgc
     9601 ctgggaatgg acacatgaac acggtggaat cattctcgaa caactaaaaa aactcggtgc
     9661 ttcgtgcgac tgggatcgta cggctttcac catggaccag aaacgctccg acagtgttat
     9721 taaagtcttc tgcaatttgt ttgataaagg attgatttat cgtggcgtgc gtatggtcaa
     9781 ctgggatccg aaagcactca cagccatttc cgatgaggaa gtaattcaca aggaagtaaa
     9841 cggtaaatta tattacctgc gctataaggt
//
LOCUS       DAOPYB010000112         9684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_8261_length_9684_cov_6.921383, whole genome shotgun sequence.
ACCESSION   DAOPYB010000112 DAOPYB010000000
VERSION     DAOPYB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8261_length_9684_cov_6.921383"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKH58_08970"
     CDS             <1..224
                     /locus_tag="PKH58_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89201.1"
                     /translation="ADNPVKFNVEGNGFVAGVDNGNQISLERFKASERKAFYGKCLVV
                     IQNSGKKGNINLKAVSEDLPPEILNLKVK"
     gene            361..3528
                     /locus_tag="PKH58_08975"
     CDS             361..3528
                     /locus_tag="PKH58_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX89202.1"
                     /translation="MMKIPLFLKNRMSSGLSSLFFTILFLFTSATFAFSQNKVTGAVT
                     SSADGEPLIGVSVHVKGTSKGTTTNYNGVYSIEVNQNQLLEFSYVGHSTKEVLITGQK
                     TVDVVLQETQETLDELVVIGYGVQKKKLNTGATLQIKGDELAKMNTTSPLQALQGKTP
                     GVSITSTSGQPGSDMKVVIRGLGTIGNSGPLYIIDGIQGDISVLNASDIQSIDILKDA
                     ASAAIYGAQAANGVVLVTTKQGSKSRSQVSFDAYTGLQNVARTAQMLNAEEYKTMMDE
                     QALNSGSALYKWNTMNDLADTDWVAQMFKKDAKTQNYSTNISGGSEASVYSLSLNYTT
                     QEGIVGGKDVSNYERYGFRVNTEHNLYKNALKVGQHLNFNYIKNTGISVGNQYNNTLR
                     GAFSTSPLAPVLSNNNIYNSPYNDTSNSLWYKGDGNPYASMMTNTNNKNDAQRLAADI
                     YAELEPIKNLKLKSVLGINYYASEYRSFTPLYQFSIYSYNKQHTTTNQSMGKGHTLTW
                     TNTAGYDFTLIQDHAISLLAGMEAVRYQGTSLSASNWNLLSQFNDFAHAYLDNTTGQA
                     HLDEDGNVVETRSVGGRPETLYRRLSYFGRVGYNFKEKYMFNATLRADGSSKFAAGHR
                     WGYFPSVSAGWVLTNEDFMSGMKGLDFLKLRASWGQVGNQDIDDFQYASPINTSTGYT
                     GSNPAAFYNFGTGGTNIAGAYPSRLSNENVKWETSEQTNIGVDARFLRNRLSVNADYY
                     IKTTKDWLVAAPILATAGAGAPFINGGDVKNNGIELALDWNDNIGGFRYNVGVNGAYN
                     KNVVGNIPTEDGIIHGLTNQLFDNAEEFYRAENGHAIGYFWGYETAGIFQNEKDIADW
                     KAAGKGILQADVKPGDVKYVDQDKSGTINSNDKIDLGNGMPSFTLGMNAGIAYKNFDF
                     SVNANGVMGNKLVQSYRNHANKQANYTTAILQRWTGEGTSDRVPRVTETNVNWQFSDL
                     YLQNGDFLRISNITLGYDFAKLIKWNYLSQFRVYGSVQNAFTFTKYDGMDPEIGYGTS
                     GWVSGIDLGYYPRPRTMLIGVNVKF"
     gene            3542..5191
                     /locus_tag="PKH58_08980"
     CDS             3542..5191
                     /locus_tag="PKH58_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX89203.1"
                     /translation="MKKTKLNIIALGIFSLTLFSCSDTFLDVEPQTSILDQNFYKTEA
                     DAEMALIGCYDGYQRTSSNGNFAFYVASEVLSDNCFGGTGNTDGRGYQAVDRFDISQS
                     PSDNNLFNGTWADYYAGIFRCNTLLKKMPGITTWTSTDEAVIGATKKRIEGETRFLRA
                     LMYFDLVRLFEKVPLLTEPTTNNVPQAEPVETYKLIVEDLKYAAANIPANAYPKAQAT
                     TNDGRVTKYAAEALLARVYLFYKGYYGKEDIGVTAAEALTGLEDIITNNEFGLVKDFK
                     ALWPAASYVASTTANTLDKTGYAGKGNKEIIFSQKFNNTQDYNGMMDGNRWLVMMGMR
                     NTNWSPYGKGWGACTVNPKLVTAFAEGDTRKPASIIDLVGEGIDSKFDKKDQREYTGY
                     TVKKYTPTCLPDGTSDTGGGNDFQISQDQDFVVIRYADVLLMAAELGSANAAGYYNLV
                     HQRAIPTAPTVAAVTKAEIMAERRFEFAFEGIRYWDLLRQGLGTAATAIAQTQNVLSG
                     TAADVVTITSGNITKTRGFMQIPNTQITLSNGVLKQNDGWK"
     gene            5211..7217
                     /locus_tag="PKH58_08985"
     CDS             5211..7217
                     /locus_tag="PKH58_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89204.1"
                     /translation="MNKIVKLFSILSLAAIMFASCSPEEFSLGKTNVTPEDLVEGIAF
                     KIEHDAANPNIVYLKSLMGPTYTPLWNHPQGRSQEQTVTLNIPFAGTYNVQFGVETRG
                     GIVYGDTVTFKVDNMFAGFISDPLWTKLSGGAGNEKTWYLDLDANSVSRYFLGPLYFY
                     GTYDGWDQVTNGIKAPEGADSWNWNPDYKGNSWLMNAADFGSMTFNLKSGANLIVNHK
                     TISSRGTQTGTYMIDVEKHTLKTQDAAILHDSNRDGVVVNWGDVKIMSLTDNTMQLAV
                     LRDPTLSGEGACLLVYNFISKDYYDNWVPPVVTEPEPQLPTGWQTDISQTVSKSIKWV
                     LSPETPFNWANLDGSLMNTDWTSADKYASWTGFNAAAAAGYAKFSLTLNSEEHTATYV
                     APDGTSTKGGYMLDDKGVYTFEGIKPNFVISGGWVTLSTTDANQWRITKIEKDPTGAV
                     SGMWVGKRDPEKAEYMVYHLIPKLGSDAPDPLAAWKKALIGKTFKPDVNWFIDWINFD
                     MTGGWTSAATFGTDYTSNGWVWNQKTSDIAKSASIKFEANGSDIKATLKQDVYDASGN
                     IATAGYTISGKVTVNPDIPSLSFEFPMVNYTGSAGAWLNSENPKGANYTKALGQNEWL
                     FVSHGGNTLSTIDTDGFWLGCVANAVAGGDSKDELLIFHFNLEK"
     gene            7330..7743
                     /locus_tag="PKH58_08990"
     CDS             7330..7743
                     /locus_tag="PKH58_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89205.1"
                     /translation="MNACVTKSQDDDAGENKKQLENTYNTVEELREKAAANPEFIHSK
                     EYASQMERAGIEMSKTTNHLKKYEVLLMEYEIALKNLKHSSDKIKSNPNLTENELFMT
                     EMQRKADKVRAYYQTLKTMKLSQMETENFIRLSHQ"
     gene            7814..9523
                     /locus_tag="PKH58_08995"
     CDS             7814..9523
                     /locus_tag="PKH58_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNX89206.1"
                     /translation="MNKFKRSFIHFLAVIQIFTLISCKEQPVIEPEIQLAKEVLDISK
                     ESQTALLAIKTNLTWTAVSSQPWCTLTPASGEAGTKQISVAVTANTAPTPREALITIA
                     AGKLSKQVKVVQALTNSLSVATKTYDIAFSGADITVAVQSTAAFTTIIQPAWITLKST
                     SGDGKTQVFTVAENTSKTPRQGIITFSLDNITETVTMRQSARDFSIPADKTGMESDAL
                     VLAKKMTLGWNLGNSLEAIGSETVWGNPKTSKALIDAVKTAGFNAVRIPCAWNGYIED
                     PVTYTIKDSWLLRVKEVVDYCVNNNMYVILNIHWDGGWLEENPTYAKQAEVNAKQKAL
                     WEQISIYFRNYDEHLMFAGTNEVHAGYGNPTPENIIVQLSYNQTFVNAVRSTGGRNTY
                     RNLVVQAYNTNIDLAVSSLKMATDNVANRMMAEVHYYDPWDFCGDTGNTAVTLWGEPF
                     KIYGKVSTWGQEADVDKQFAKMKTNFVNKGYPVILGEYAPTRRLSLSGEALTHHLDSR
                     AYYLKYVTQQAKNNGIVPFYWDNGAIGDNGSGIFNRSNGTIADDKAYNALLQGSAAGI
                     YPY"
BASE COUNT         3008 a         2046 c         2118 g         2512 t
ORIGIN      
        1 atgctgataa tccggtgaaa ttcaacgtag aaggcaacgg ctttgtggcc ggagttgata
       61 atggaaatca gatcagcctg gaaagattta aagcatccga gcgaaaagca ttttatggaa
      121 aatgtctggt tgtcattcaa aattcgggca agaaaggaaa tatcaatttg aaagcagtct
      181 ctgaagatct acctccagaa attttgaatt tgaaagtgaa ataagttgcc gaaacaaaca
      241 caataataaa aaactatgaa tactaaccat taaaaaacaa aacaccccat gaaaaacaat
      301 ttctttaaat tttcaatttc ttttacagtt atcaatgttt aaccttcaaa acattcaaaa
      361 atgatgaaaa taccattatt tcttaaaaat cggatgagct cgggcttatc ttcactcttt
      421 tttacaattt tgtttctgtt tacatcagct acttttgcat tttcacaaaa taaagtgaca
      481 ggtgcagtta cctcatcggc cgatggtgaa ccgcttattg gcgtgagtgt acacgtaaag
      541 ggaacatcca aaggaacaac aactaattac aatggtgtat attccattga agtcaatcag
      601 aaccaactgc ttgagttctc atatgttggt cattcgacca aagaagtact catcacagga
      661 caaaaaaccg tagatgtggt actgcaggaa acacaggaaa ccctggacga attggtagta
      721 atcggttatg gcgtacaaaa gaaaaaactt aacaccggag ctaccttaca gatcaaagga
      781 gacgaactgg ccaaaatgaa taccaccagt ccgctgcagg ctttacaggg caaaactccc
      841 ggtgtaagca tcacatctac ttcgggccag cccggatccg acatgaaggt tgttatccgt
      901 ggtctcggaa ccattggaaa ttcaggtcca ctttacatta ttgatggtat tcagggcgac
      961 atctctgtcc taaatgcatc ggatattcag tctattgaca ttcttaagga tgcggcttca
     1021 gctgctattt atggtgctca ggctgccaac ggagttgtgc tggtaactac caaacaaggc
     1081 tccaaaagtc gcagtcaggt ttcgttcgat gcttataccg gcctgcagaa cgtagcacgt
     1141 actgcgcaaa tgctgaatgc tgaagaatat aaaacaatga tggatgaaca ggcattgaat
     1201 tcaggttctg ccctttacaa gtggaataca atgaatgatc tggctgatac cgactgggtg
     1261 gctcagatgt ttaaaaaaga tgctaaaact caaaactatt caacaaatat cagtggtggt
     1321 tctgaagcat cggtttattc cctgtcgttg aattatacta ctcaggaagg cattgtaggc
     1381 ggtaaggatg tttcgaatta cgaacgctac ggatttcggg tgaacacaga acataacctt
     1441 tacaaaaatg cccttaaagt aggtcagcac ctcaatttca actatatcaa aaacacaggt
     1501 atcagtgttg gcaatcagta caataataca ttgcgcgggg cattttccac atctccactt
     1561 gcgcctgtac tcagcaacaa caatatctac aacagtccat acaacgatac aagtaactcg
     1621 ctgtggtaca aaggcgatgg caatccttac gcaagcatga tgactaatac caataacaaa
     1681 aacgatgctc agcgactggc tgccgatatt tacgccgaac tcgaacccat taaaaattta
     1741 aagttaaaat cagttttagg cataaattac tacgcttcgg aataccgcag tttcaccccg
     1801 ttgtatcagt tttccatata ttcttacaac aagcagcata caactaccaa tcagagcatg
     1861 ggcaaaggcc atacacttac ctggaccaat actgctggtt atgactttac actcatccag
     1921 gatcatgcca tcagtttgct ggctggtatg gaagctgtgc gctatcaggg aacaagcctc
     1981 tcagcatcaa actggaatct gttatctcag ttcaacgatt ttgctcatgc ttatctcgat
     2041 aataccaccg gtcaggcgca cttagacgaa gatggtaatg tagtggaaac ccgttcagta
     2101 ggaggcagac ccgaaacatt gtatcgccgt ctatcctatt ttggacgtgt gggctacaac
     2161 ttcaaagaga aatacatgtt taacgctacg ttacgtgccg acggatcatc caaatttgca
     2221 gccggtcatc gctggggata ttttccatcg gtatcagcag gttgggtgct caccaatgaa
     2281 gattttatgt ccggaatgaa agggcttgac ttcctgaaac tccgcgccag ctggggacaa
     2341 gtgggtaatc aggatatcga cgattttcag tatgcttctc caatcaatac atccacaggt
     2401 tataccggaa gcaatccggc agcattttac aactttggaa caggtggaac aaatatagcc
     2461 ggtgcatatc caagtcgctt gtccaacgaa aatgtgaagt gggaaacatc cgaacaaact
     2521 aacatcggtg tggatgctcg tttcctgcga aatcgcttat cggtcaacgc cgattattat
     2581 atcaaaacca ccaaagactg gcttgtagca gctcctattc ttgccactgc tggtgcaggg
     2641 gctccattta tcaatggtgg agatgtgaaa aacaacggta ttgaactggc tcttgactgg
     2701 aacgacaata ttggaggttt tcgatacaat gtaggcgtta acggtgctta caataagaat
     2761 gtggtaggaa atatacctac cgaagatgga attattcacg gattgacgaa tcaacttttt
     2821 gataatgccg aagaatttta ccgtgccgaa aacggtcatg ccattggcta tttctggggg
     2881 tatgaaactg ccggaatttt tcagaatgaa aaagatattg ccgactggaa agctgccggc
     2941 aaaggaatac ttcaggccga tgtaaaacca ggcgatgtga aatatgtgga tcaggataaa
     3001 agtggcacca tcaattcaaa tgataaaatt gatctgggca acggaatgcc gtcctttaca
     3061 cttggaatga acgccggaat tgcctataaa aactttgatt tttcggtgaa tgccaatgga
     3121 gtaatgggta ataaactggt gcagtcctat cgcaatcatg ccaataaaca ggccaattat
     3181 acaaccgcta ttcttcagcg atggacaggt gaaggaacct cagacagagt accgcgcgta
     3241 accgaaacca acgtcaactg gcaattttca gacctctatc tgcagaatgg cgacttcctg
     3301 cgtatcagca atatcaccct cgggtacgat tttgccaaac tcatcaaatg gaattacctg
     3361 agccagttca gggtgtacgg atctgttcag aatgcattta cttttaccaa atatgacggt
     3421 atggatcccg aaataggtta tggcacaagc ggatgggttt cgggtataga cctcggatat
     3481 tatccaagac ctcgaacaat gctgattggt gttaatgtta aattctaaaa aatccataat
     3541 aatgaaaaag actaaattaa atattatagc attgggaatt ttcagcttga ctttattctc
     3601 ctgctcagac acttttctcg atgtggaacc acagacctcc atactcgacc agaacttcta
     3661 caaaacagaa gcagatgccg aaatggcgtt gattggctgc tacgacggct accaacgtac
     3721 atcctccaat ggtaactttg ctttctatgt agcctccgaa gtactctccg acaattgttt
     3781 cggtggaaca ggaaatacag acggacgtgg ctatcaggct gtcgaccgct tcgatatttc
     3841 gcagtcacca tccgacaata atttattcaa cggaacctgg gccgattatt acgccggtat
     3901 tttccggtgc aatacattgc tgaaaaaaat gccagggatt acgacatgga catcgaccga
     3961 tgaagctgta ataggggcaa ccaaaaaacg tattgaagga gaaacccgct tcctgcgtgc
     4021 tctcatgtat ttcgatcttg tgcgattgtt cgaaaaagtg cctctactca ccgaacctac
     4081 taccaataat gtgccgcagg ccgaaccggt ggaaacctat aaattgatag tggaagacct
     4141 caaatatgcg gctgccaata tccctgccaa tgcttatccc aaagcccagg cgactaccaa
     4201 cgacggacgt gtaaccaaat atgcggctga agctttgctg gcacgggtat acctatttta
     4261 caaaggctat tacggtaagg aagacattgg cgtaactgct gccgaagcgc tcactggtct
     4321 ggaagatatt attacaaata acgaattcgg actcgttaaa gatttcaaag ccctttggcc
     4381 tgcagcaagt tatgtagcca gtactacagc caatacgctg gataaaaccg gatatgccgg
     4441 aaaaggaaac aaagaaataa ttttttcgca gaaatttaac aatactcagg actacaacgg
     4501 catgatggac ggtaaccgct ggctagtgat gatgggaatg cgtaacacca actggtcgcc
     4561 ctacggaaaa ggctggggag cttgtactgt aaatcccaaa ctggtgacag catttgccga
     4621 aggcgataca cgtaaaccgg cttccatcat cgatttggta ggtgagggta tcgacagtaa
     4681 atttgacaag aaagaccaac gggaatatac cggatatacg gtaaaaaaat acaccccaac
     4741 ctgcttaccc gatggaacat ccgataccgg tggcggcaat gactttcaga tatcacagga
     4801 tcaggatttt gtagttattc gttatgccga cgtattgctt atggcagccg agcttggaag
     4861 tgccaatgca gcaggatatt acaacctggt acaccaacgt gctattccca cggctccgac
     4921 tgtagcggca gtaaccaaag ctgaaattat ggctgaacgc cgctttgaat ttgcattcga
     4981 aggaatccgc tactgggatt tgttgcggca gggactcggc acagctgcta ctgccattgc
     5041 tcagacacaa aacgtgctca gtggaactgc tgccgatgtg gtgacaatta cttcaggtaa
     5101 tattaccaaa actagaggct tcatgcagat acccaatact cagattacac tttccaatgg
     5161 agtgttgaaa caaaatgatg gctggaaata aacattaata cgatactaaa atgaataaaa
     5221 tagttaaatt attttcaata ttgtcgctgg cggcaatcat gtttgcatcc tgttcgcccg
     5281 aagaattttc tctcggtaaa accaatgtta cacccgaaga tcttgttgaa ggcattgctt
     5341 tcaaaattga acacgatgca gcgaacccca atatcgttta tcttaaaagc ctgatggggc
     5401 cgacttacac accgctttgg aatcatccac agggacgaag tcaggaacaa acggtaacac
     5461 ttaatattcc atttgcaggc acttacaatg tgcagttcgg tgtagaaacc cggggtggaa
     5521 tagtgtatgg cgatactgtt acatttaagg tggacaacat gttcgccgga tttatcagcg
     5581 acccgctctg gaccaaactt tcgggtggtg cgggaaatga aaaaacctgg tatctggacc
     5641 ttgatgccaa ctctgtaagc cgatactttt tggggccact ctatttttac ggtacgtatg
     5701 atggctggga tcaggtaacc aatggtatta aagctcccga aggagccgat tcgtggaact
     5761 ggaatcccga ttataaggga aattcatggc tcatgaatgc agccgatttt ggatcaatga
     5821 ctttcaacct gaaaagtgga gcaaatttga tagtgaacca taaaaccatt tcgtcacggg
     5881 gaacccaaac aggtacgtat atgatagatg tggaaaaaca cacactcaaa acacaggatg
     5941 ctgccattct tcacgacagc aaccgggatg gtgtggtggt taactggggt gatgttaaaa
     6001 tcatgtcgct caccgacaat accatgcaac ttgctgttct ccgcgatcct acactatcag
     6061 gcgaaggtgc ttgcttactg gtttacaatt ttatttccaa agattattac gacaactggg
     6121 ttccgccggt agttactgaa ccagaaccac aactgcccac cggttggcaa acagacatat
     6181 cccagacggt atccaaatcc ataaagtggg ttctttctcc cgaaactcct ttcaactggg
     6241 ctaatctgga tggatcgctg atgaacaccg actggacatc ggccgataaa tacgccagct
     6301 ggacaggttt caatgccgct gccgctgccg gttatgctaa attctcattg acactcaatt
     6361 ctgaggaaca taccgcaact tatgtggcac cagacggtac ctccaccaaa ggtggctata
     6421 tgctggacga taaaggggta tatacttttg aaggaattaa gccaaacttt gtaatcagcg
     6481 gtggctgggt aaccctgagt actaccgatg ccaaccaatg gcgtataacc aagattgaaa
     6541 aggatccaac aggtgctgta tcaggtatgt gggttggaaa acgagatcct gagaaagccg
     6601 aatacatggt ttatcattta attcccaaac tgggaagcga tgcaccggat ccgcttgcag
     6661 cctggaaaaa agctttgata ggtaaaacat tcaaacccga tgtcaattgg tttatcgact
     6721 ggataaactt tgatatgacc ggtggatgga ccagtgctgc gacatttgga accgactata
     6781 caagcaacgg atgggtatgg aaccagaaaa cgagcgatat tgccaaaagt gccagtatca
     6841 aattcgaagc aaacggttcg gatataaaag ccacactcaa acaggatgtg tacgatgcga
     6901 gtggaaatat agccacagcg ggctacacca tttcgggcaa agtgactgtt aatcctgata
     6961 taccctcact tagcttcgaa ttcccgatgg taaattatac cggtagcgcc ggtgcatggt
     7021 tgaattctga aaatccgaaa ggggctaact ataccaaagc tcttggtcag aacgaatggt
     7081 tgtttgtttc acacggaggt aatacgctca gtaccatcga taccgatgga ttttggctgg
     7141 gatgtgttgc caatgcagtg gccggaggcg attccaaaga tgaattgttg attttccact
     7201 ttaatttaga gaagtagttc acttaaaaga gattgcctgt aaacagggca atctcttttt
     7261 aatctttttt tgttaatgaa atatcgactt gattttagaa taatattcat attatttact
     7321 gtaataataa tgaatgcctg tgtaacaaaa tctcaggatg atgatgctgg tgagaataaa
     7381 aaacaacttg aaaatacata taatacggta gaagaactaa gagaaaaagc agccgcaaat
     7441 ccagaattta ttcattccaa agagtatgcc tctcaaatgg aaagagcggg aatcgaaatg
     7501 tcgaaaacca caaatcatct gaaaaagtat gaggtgctgc tgatggaata cgaaatagca
     7561 ttaaaaaacc tgaagcatag ttcggataaa attaaatcaa acccgaattt gactgaaaat
     7621 gaattattta tgaccgaaat gcagcgcaag gctgacaaag taagggcata ttaccaaacg
     7681 ttgaaaacaa tgaaactcag tcagatggaa accgaaaatt tcattcggtt atcgcatcaa
     7741 tgatagtaaa cccgataata tatcaataaa atgatctttt tttcaaatca atcatttatc
     7801 atcagattat attatgaaca aatttaaaag atctttcatt cattttttag cagtaattca
     7861 gatttttact ttaatttcct gcaaggaaca accggtcatt gaaccagaaa tacagttggc
     7921 taaggaagtg ctggatatat cgaaggaaag tcaaacagcc cttttggcca tcaaaaccaa
     7981 tctaacctgg acagctgtca gttcacaacc ctggtgtacg cttactcccg cttcgggcga
     8041 ggccggtacc aagcaaattt ccgtggctgt tacggctaat accgcaccta cacctcgtga
     8101 agctctgatt acaattgctg ccggaaaact atcaaaacag gtaaaagtgg ttcaggcttt
     8161 gaccaattcg ctcagcgtag ccaccaaaac ttatgatatt gctttttcag gagccgatat
     8221 tacggttgct gttcagtcca cagcagcatt cactactatc atccagcctg cgtggataac
     8281 gctgaaatca acttcaggcg atgggaaaac gcaggttttc accgttgctg aaaacacttc
     8341 aaaaactcct cgtcagggta tcatcacctt ttcacttgat aatattaccg aaaccgtcac
     8401 tatgcgtcaa tcagcaagag atttcagtat tccggccgat aaaacaggga tggaaagcga
     8461 tgcgctggta ttagccaaaa aaatgaccct gggatggaat ttgggcaatt cgctcgaagc
     8521 tattggcagt gaaacggtgt ggggtaatcc gaaaacatca aaagcattga ttgatgctgt
     8581 aaaaactgcc ggctttaacg ccgttcgcat tccatgtgcc tggaatggat acatcgaaga
     8641 tccggtaaca tatacaatta aggacagctg gctcctacgg gtgaaagaag tggttgatta
     8701 ttgtgtgaat aataatatgt atgtcatcct gaatattcat tgggatggtg gttggcttga
     8761 agaaaatccc acttacgcaa aacaagcgga ggttaatgcc aaacaaaaag cattgtggga
     8821 acaaatttca atctatttcc gaaactatga cgaacattta atgtttgccg gtactaacga
     8881 agtacatgca gggtatggta atcccacgcc cgaaaatatt atcgttcaac tctcatacaa
     8941 tcagactttt gttaatgccg tccgttctac cggagggcga aatacgtatc gcaatcttgt
     9001 tgttcaggcc tataatacca acatcgatct ggcagtgtcc agtctgaaaa tggctaccga
     9061 caatgtggct aaccgcatga tggctgaagt gcattattac gatccgtggg atttttgcgg
     9121 tgatacggga aacactgctg ttaccctgtg gggtgaacct tttaagattt acggtaaagt
     9181 atcaacctgg ggacaggaag ccgatgtgga taagcagttt gccaaaatga aaactaattt
     9241 tgttaacaag ggatatcctg taattttggg tgaatatgct cccaccaggc gattatctct
     9301 ttcgggtgaa gcactcacac atcatctcga ttcaagggct tactatctca aatatgttac
     9361 ccaacaagct aaaaataacg gtattgtgcc attttactgg gacaacggtg cgatcggaga
     9421 taatggttcc ggaattttca accgcagtaa cggtaccata gccgacgata aagcatacaa
     9481 tgccctgcta cagggaagtg cagcaggtat ttatccatac tgatttataa tcagtgttta
     9541 ttaaagtttt tttttcatgg aaaagaaaat ctctgggagc gtcctaccgg ggatttttga
     9601 tgataagcca ttacagtttc tgagtatcgg gacggttttt ctccgaatat tccttgggcg
     9661 acaggccata gtaatcctta aatg
//
LOCUS       DAOPYB010000113         9664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_8287_length_9664_cov_9.126756, whole genome shotgun sequence.
ACCESSION   DAOPYB010000113 DAOPYB010000000
VERSION     DAOPYB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8287_length_9664_cov_9.126756"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2078
                     /locus_tag="PKH58_09000"
     CDS             <1..2078
                     /locus_tag="PKH58_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89207.1"
                     /translation="LLLLSGIGFTSCDKDEVDDTILLNSFGPSPALRGGDLRFIGNNL
                     DKVTSIVLPGLTSGSTIEVSDIAVVDEREIKITIPQNAGVGLVTLKTPQGDIKTKTPL
                     TYSEPISIESVSPATIKAGQVLTIKGDYLNLIKRVIFFDGVAVDDTAFVSRTRKQIEL
                     KVPVQAQTGKIILSNGAEIPIEVYSKEALNVVLPTFTSFAPTTIKPGAVLTITGKDLD
                     LVDMVEFGGGKIIPSNNFTLNTAGTEIKVTVPEDAHDGAVNLLAKSGVVVENGATLTT
                     VMPSGLSVTPDPIKNNTEITVKGANMDLVSAVKFGDLEAVISSKAADKIVVTVPKTAI
                     ATKATLVTLSTKTIETPAFTYIKPAMTSLNPTTLMAGSELTIEGTNLDLVKQVKFPSS
                     AKIVDVTPASANSFAVTVPTDATSGNPVLVTINGTEVESTISLTVTAADIPVITDIPA
                     TVKPGKLLVMKGTKLNLVKSVIFENNVEATNFDTRSATLLEVYVPDGAKAGLNNIKLV
                     TYSGKVAMTSVTISGTDPITSQTKMIYDFNQRTSDWHAVDWDNWGGSYDAGLGKTNGY
                     ITLFSRPGWWILGCNHPDPNGGWPSVNPNNYVLKVDIKTIKPILITGGYEFIFKIGGE
                     NVSSQLIVSGNYIATPDNDWATLTIPISGVLSNPTKNSGDFGIVLNSSDAGTDFAGLC
                     FDNIRFDPK"
     gene            2147..3493
                     /locus_tag="PKH58_09005"
     CDS             2147..3493
                     /locus_tag="PKH58_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNX89208.1"
                     /translation="MKSLFKFIFIGLLLLASCKPDPEPEEITPPQLVSVDPANGAMDV
                     SENMTRIVMTFNENVTLKTGYNITLNGSPVSDVQPGTNKELTITVSSLSVSTSYTLII
                     PANTVKGPTGVYLPTEIKITFKTYTPPVRTYSTEAQKVIDFLKSIYGTKTISGTMANV
                     NWNTDEADWVKVQTGKYPALNCFDYVHHVFSPANWIDYSNTTVAENWWNNNGLVAIMW
                     HWNVPKNINSGSSTEYAFYASNTNFDLSKISDTNSAEYKLIIKDIDIIAGYLKLLKNK
                     NIPVIWRPLHEAAGNTNSYTGGKAWFWWGAKGAAPFKALWKLMFERLTTTHQLNNLIW
                     VWTSQGNDPDWYPGDEYVDIAGRDLYPETNVHDSQIVEFNKVKTIINDHKMIALSECS
                     GIPDPDKMMQNGDTWLWFMPWYGDYTRTDKYNGSTYWKSLFNNPAVITRDKMPSLK"
     gene            3557..4639
                     /locus_tag="PKH58_09010"
     CDS             3557..4639
                     /locus_tag="PKH58_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNX89209.1"
                     /translation="MSCNKKIAVGKTAVPNTDNELTPETACLLKNLMSIPEKGFLFGH
                     HDDPLYGVGWKGDNDRSDIKSITGDYPAVMSFDLGRIELEQQSNIDKTNFDKIRQEII
                     RQYNRGGMISVSWHINNPLTDGDAWDAKTPGAVNSVLPGGNNHSKFMKWLQSAAGFLK
                     TLKTSNGTKIPIVFRPWHEHTGSWFWWGKGNCTAEEYKQLWIMTYRFMKNEGLNNLLF
                     AYSPDIQGPGDIYLEFYPGDKYVDILGLDGYHRDNEKGTEAYTKALNTILAFMTEEGK
                     KRHKPIALTETGLEAIPIADWWTNVLLPIVEKYPVSYVLVWRNAYDRENHYYAPYPGQ
                     ISEADFKKFYLNSKTLFCKDVQNLYK"
     gene            4652..5821
                     /locus_tag="PKH58_09015"
     CDS             4652..5821
                     /locus_tag="PKH58_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HNX89210.1"
                     /translation="MNDFNNKIKQLFENQEKFLNVENTPVVNTNGWYQRYKNPILTAA
                     HIPLNWRYDLSEQTNPLLMERIGINAVLNSGAIKWEGKYLIVARVEGYDRKSFFAIAE
                     SPNGIDNFRFWSHPVLMPDTENPTTNVYDMRLTQHEDGWIYGIFCAERPDPTAAYGDL
                     SKATAAAGIARTKDLIHWERLPDLKSDSQQRNVVLHPEFVNGKYALYTRPQDGFIDTG
                     KGSGIGWALVDSMENAVVTEEIIIDKRFYHTIKEVKNGEGPSPIKTPYGWIHLAHGVR
                     NCAAGLRYVLYLYMTSLEDPTLQIAAPGGYFMAPEEEERVGDVSNVLFSNGWIADDDG
                     RVFIYYASSDTRMHVATTTIDRLVDYCLNTPEDGLTTTASVQVINKLIDKNLQVQ"
     gene            5836..7278
                     /locus_tag="PKH58_09020"
     CDS             5836..7278
                     /locus_tag="PKH58_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX89211.1"
                     /translation="MAQIKFKEKAGYGFGDMASSMFWKIFGMYSLFFYTDVFGISAAA
                     AGTMFLVARLWDSFFDIGVGIMADRTKTRWGKYRPYLLWFAIPFSVMGVITFFVPDFG
                     QTGKLIYAYITYSLMMIVYSLINVPYASLLGVMSPDPKMRNVLSSYRMTFAFIGSFIT
                     FMLLQPLVDFFSQTFDSASLEKTGEGLQTAVHASVSNSPVGWTLGVLVIGAICSVLFF
                     LCFSLTRERVEAVSKEENENISVKQDLQHLLHNSPWWILVGTGLAALLFNAVRDGVAI
                     YYFRDYVGSNYTAPLFGWDMATIYFLVGQAANIVGVVLAPSVSARFGKRHTYMLAMVI
                     AAVLSIVFFFIPNNVTLILILQVLISVCAGYVLPLLWSMFSDIVDYQEYKTNRRASGL
                     IFSSSSMSQKLGWALGAALGGWILAIFKYNPEIVTQLPQTIFGERLMLSVFPAICALL
                     AAVGMILYPLTEDKVKQIAEELNKRREDAK"
     gene            7268..8467
                     /locus_tag="PKH58_09025"
     CDS             7268..8467
                     /locus_tag="PKH58_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNX89212.1"
                     /translation="MQSNTARLLKEAEQIFTENISPFWLSKMVDDEHGGFYGRITGEG
                     EIFPDAPKGAVLNARILWTFAAAYRIFGEESYLIAARRAKEYLLKHFYDITDGGIYWM
                     LDYTGKPLETKKQIYAQGFAIYGLSEYYRATSDNEAIECAKAIFFDIEKHSFDKLGNG
                     YLEAFTENWQPIADMRLSEKDENVEKTMNTHLHILEPYTNLYRVWKNEKLKHQLQNLI
                     ELFLDKIINPDNYHLNLFFDMDWTDKSEIISYGHDIEASWLLYEAALVLGDEKILKRV
                     KELIPHIIEASLEGFQPDNSMIYEKEENSGDEDRDRHWWVQAETVVGLLNHYQLYNDI
                     NSLNKAIACFDYIKNNLQDKENGDWFWSIRSDGTVNRSDDKAGIWKCPYHNSRMCFEI
                     MERFGRE"
     gene            8519..9505
                     /locus_tag="PKH58_09030"
     CDS             8519..9505
                     /locus_tag="PKH58_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNX89213.1"
                     /translation="MKHFLLIMLSLVSILTFSSFKAPRNTPVAVHGNLKVTGTQLVDQ
                     QDQSVMLRGTSFGWHNLWPRYYNKKAVSWLVKDWKCNVIRASMGVALDDSYLENPDFA
                     LKCMTTVIDEAIKCGIYVIIDFHSHKIHTAEAKEFFTLMAGKYGKYPNVIYEIFNEPD
                     YYSWEEIKTYSEELISTIRTVDPDNIILVGSPHWDQDLHLVAENPIKGQNNIMYTMHF
                     YAGTHKKWLRDRTDEAIATGIPVFVSECAGMEASGDGPIDDEEWNSFLSWMEEKKISW
                     IVWSVSDKNETCSMLLPRASSEGNWADDVIKKWGLLGRDAIRKGNQNNTSNH"
BASE COUNT         2944 a         2056 c         2062 g         2602 t
ORIGIN      
        1 gcttattgct gctatccgga attggattta cttcatgcga caaagatgaa gtggacgata
       61 ccatcctgct caattcattc ggtccttcac cggcactgcg cggtggtgac ctgcgattta
      121 tcggtaataa tctggataaa gtaacctcca tcgtacttcc gggcctgaca tcaggcagta
      181 ctattgaagt cagcgacatt gctgtcgttg acgagcgcga aatcaaaatt actatccctc
      241 agaatgccgg tgttggcctt gtaactctaa aaacacctca gggtgacata aaaaccaaga
      301 cccccctcac ctattcggaa ccaatctcca ttgaaagtgt ttctccggca acaatcaaag
      361 ccggacaagt actgaccata aaaggtgatt atctgaattt gattaaaaga gtaattttct
      421 tcgatggcgt tgctgtggat gatactgctt tcgtgtcaag aacccgcaaa cagatagaac
      481 tgaaagtacc tgtacaggct caaaccggaa aaattattct ttccaacggc gccgaaattc
      541 ccatcgaagt ttactcgaaa gaagctctga atgtggtttt accaacattt acctcattcg
      601 ctccaactac cattaagccc ggtgctgtac tgacaattac cggtaaagat ctcgacctgg
      661 ttgatatggt cgaatttggc ggtggaaaaa tcattccgag caataacttc acactgaaca
      721 ctgccgggac tgaaataaaa gtgactgttc ctgaagatgc tcatgatgga gccgtgaatt
      781 tactggctaa atcgggagtt gttgttgaaa acggagcaac gctgaccaca gtcatgcctt
      841 caggcttaag cgtgaccccc gatccaataa aaaataatac tgaaattacg gttaaaggtg
      901 caaatatgga tctggtttca gctgtaaaat ttggtgatct ggaagctgta ataagttcaa
      961 aagctgctga taaaatagtt gtaactgttc caaaaactgc catagccact aaagccacgc
     1021 tggtaacttt atcgactaaa accattgaaa caccggcatt tacatatatt aaaccagcga
     1081 tgacctcatt gaatcctacc actctgatgg caggcagtga actgaccatc gaaggtacca
     1141 atctggatct ggtaaaacag gtaaaattcc cttcatcggc aaaaattgta gatgtaacac
     1201 ctgcttcagc caattcattt gctgtaacag tacctaccga tgctaccagt ggtaaccccg
     1261 tattagttac cattaacgga acagaagtag aatcgactat ttcattgact gtaacagctg
     1321 ccgatatacc tgtgattact gatatacctg caaccgttaa accgggcaaa ctgcttgtaa
     1381 tgaaaggtac taaactgaat ttggtaaaat cggtgatttt tgaaaacaac gtggaagcaa
     1441 ccaattttga tacccgttcg gcaacactgc tcgaagtgta tgtgcctgat ggagcaaaag
     1501 ctggtctgaa caacatcaaa ctggttactt actccggaaa agttgctatg acctcggtaa
     1561 ctatcagcgg taccgacccc ataacttcac aaaccaaaat gatctacgat ttcaaccagc
     1621 gtacctccga ctggcatgca gttgactggg ataactgggg aggttcttac gatgcaggtc
     1681 ttggtaaaac aaacggttat atcacgctct tctcgcgtcc gggatggtgg atattaggtt
     1741 gcaatcaccc cgatccgaac ggtggctggc cttcagtaaa tccgaataac tatgttttga
     1801 aggtagatat taaaaccatt aaaccaattc tgattacagg tggatatgaa tttattttca
     1861 aaattggcgg tgaaaatgta tcttctcagc tgattgtttc gggcaattac attgcaactc
     1921 ccgataacga ctgggctaca ttaaccatac caatcagcgg agtattgtca aatccgacaa
     1981 aaaattccgg agatttcggt atcgttctta actcctcgga tgcaggaaca gattttgccg
     2041 gtctgtgctt tgataatatc cgtttcgatc ctaaataatt atattacttc aaaacagaag
     2101 agagcgtaaa aaactctctt ctgttcaatt caaaatattc ctgacagtga aatcactatt
     2161 caaattcata ttcataggtc tgctcttgct ggcttcatgc aaacccgatc cggagcctga
     2221 agaaataaca ccgcctcagc ttgtgtcggt cgacccggct aacggtgcaa tggatgtttc
     2281 tgaaaatatg acccgaatag tgatgacttt taacgaaaat gtaactctga aaactggtta
     2341 caacatcacc ctgaatggtt ctccggttag tgatgttcag cccggcacga acaaagaact
     2401 gacaatcacc gtttcttctc tatccgtttc gaccagttat acgcttatta taccggcaaa
     2461 tactgtcaaa ggtccgacag gagtatatct cccgacagaa ataaaaatta ctttcaaaac
     2521 ctacactcct cccgtaagga cttattcaac agaggcacag aaggttatag attttctgaa
     2581 aagtatatac gggacaaaaa cgatttcggg aaccatggcc aatgtcaatt ggaataccga
     2641 tgaggcagac tgggtgaaag ttcaaaccgg aaaatatcct gccctgaatt gcttcgatta
     2701 tgttcaccat gttttctctc ctgccaactg gatagattat tcaaatacta cggttgctga
     2761 aaactggtgg aacaacaacg gactggttgc aatcatgtgg cattggaatg taccaaaaaa
     2821 tatcaactcc ggaagtagta ccgaatatgc cttttatgcc agcaacacca attttgattt
     2881 aagcaaaatc agcgacacaa actcggctga atacaaactg attattaagg acattgacat
     2941 catagccgga tatcttaaat tgttgaaaaa caaaaacata ccagttatat ggcgaccact
     3001 gcatgaagct gccggcaaca ccaactccta tacgggtggt aaagcctggt tctggtgggg
     3061 agcaaaaggt gcagctccgt tcaaggcact ctggaaactg atgtttgagc gtcttaccac
     3121 aacacatcag ctaaataacc tgatatgggt atggacctcg cagggaaatg atccggactg
     3181 gtatcccggc gatgagtatg tcgacattgc aggacgtgat ttataccccg aaacaaatgt
     3241 tcatgactcg cagattgttg aattcaacaa ggtaaaaacc atcattaatg atcataaaat
     3301 gattgccttg tccgaatgca gcggaatacc cgatcctgat aaaatgatgc aaaacggtga
     3361 cacctggttg tggtttatgc cctggtatgg agactatacg cgtaccgaca aatacaatgg
     3421 ttcaacctat tggaaatcac tttttaacaa tccggcagtt ataacccgag acaaaatgcc
     3481 ctcattaaaa tagcataaca acatgaaaat aaaactgatt ggtttattga tatttttatc
     3541 aataaatata ctttttatgt cttgtaataa gaaaattgcc gtcgggaaaa cagcagttcc
     3601 gaacactgat aatgagctca cacccgaaac cgcctgtctt ttaaaaaacc tgatgtcaat
     3661 tcccgaaaag ggttttctgt tcggtcatca cgatgacccg ctttatggtg taggctggaa
     3721 aggcgataac gacaggtctg atataaaaag cattacaggc gattatcccg cggtcatgag
     3781 tttcgatctg ggcagaatag aacttgaaca gcaaagcaat atcgataaaa ccaatttcga
     3841 taaaatcagg caggaaatca ttcgtcagta caaccgtggt ggaatgatat ctgtgagctg
     3901 gcatatcaac aatccgctaa ctgacggcga tgcctgggat gcgaaaacac ccggtgctgt
     3961 aaattcagtt ttacccggag gcaataacca cagtaaattt atgaaatggc tgcaatcagc
     4021 cgccggattt ctgaaaacac tgaaaacttc caatggtaca aaaataccta tcgttttccg
     4081 cccctggcac gaacacactg gaagctggtt ctggtgggga aaaggaaact gcactgccga
     4141 agaatataaa caactctgga tcatgactta ccgtttcatg aaaaacgaag gactgaataa
     4201 tctgctgttc gcctactctc ccgacattca gggtccgggc gatatatatc tggagtttta
     4261 tccgggcgac aaatatgttg acattcttgg tcttgacggt taccaccgcg ataacgaaaa
     4321 gggtactgaa gcttacacca aagctctcaa caccatcctg gctttcatga cagaagaagg
     4381 caaaaaacgt cataaaccca tagcacttac cgaaaccggg ctggaagcca taccaattgc
     4441 cgactggtgg acgaatgtac ttttacccat agttgaaaaa tatcctgtaa gttacgtatt
     4501 ggtttggaga aatgcttatg accgtgaaaa tcattactat gcaccctatc caggtcaaat
     4561 atcagaagcg gattttaaga aattctattt gaattcaaaa actctgtttt gtaaagatgt
     4621 tcaaaattta tacaaataaa acatatacaa gatgaatgat tttaataata aaattaagca
     4681 gctgtttgaa aatcaggaaa aattcctgaa tgtagaaaac acccctgtag tgaatactaa
     4741 cggatggtat cagcgatata aaaatccaat actcactgcc gctcacattc cgctcaactg
     4801 gcgttatgat ctcagcgagc aaacaaatcc gttgctgatg gaacgaatcg gcatcaatgc
     4861 tgtgcttaat tcgggagcca taaaatggga aggaaagtac ctgattgttg cccgcgtgga
     4921 aggttatgac cgcaaatcgt tttttgcaat cgccgaaagt ccgaatggca ttgacaattt
     4981 ccggttctgg tcgcatccgg tgttgatgcc cgacactgaa aatccgacaa ccaatgtata
     5041 tgatatgcgg ctgacacaac acgaggatgg ttggatatac ggtattttct gtgcagaaag
     5101 gcctgatcct acagctgcat acggcgattt gtcgaaagcc actgctgcag caggcattgc
     5161 ccgcacgaaa gatcttattc actgggaaag attgccagat ctgaaatcgg acagtcagca
     5221 acgcaatgtg gtgcttcatc ctgaatttgt aaacggtaaa tatgcgcttt acacccgtcc
     5281 gcaggatgga tttatcgata ccggcaaagg ttccggaatt ggctgggcgt tggtcgatag
     5341 tatggaaaat gctgttgtaa cagaggagat tatcattgac aaacgttttt atcataccat
     5401 caaagaagtg aaaaacggag aaggaccctc acctataaaa actccgtacg gatggattca
     5461 tttggcacat ggtgtaagaa attgtgctgc aggtctgaga tatgtattat atttgtacat
     5521 gacttcactc gaagatccaa ctctgcaaat agctgcaccg ggcggatatt tcatggcacc
     5581 cgaagaagaa gaacgtgtgg gtgatgtatc aaatgttttg ttttcgaatg gctggattgc
     5641 cgatgatgac ggtcgcgtgt ttatctatta cgcttcgtcg gacacccgta tgcatgtagc
     5701 aacgacgact atcgacagac tggtggatta ctgcctgaat acaccggaag acggcttgac
     5761 aacaacagcg tccgttcagg taattaacaa actgattgac aaaaatttac aagttcaata
     5821 aaataactga taccaatggc acagattaag tttaaagaaa aagcaggtta tggatttggc
     5881 gacatggcat catccatgtt ctggaaaatc ttcgggatgt attcattgtt tttctatacc
     5941 gatgtattcg gaatttcagc ggcagctgcc ggaacaatgt ttcttgtagc acggctctgg
     6001 gactcgttct tcgatatcgg tgtcggaatt atggccgatc gcaccaaaac ccgctggggt
     6061 aaataccgtc cctacctgct gtggtttgcc ataccttttt ccgttatggg agtcataaca
     6121 tttttcgttc cagatttcgg acagacaggc aaactaatat atgcatacat cacctattcg
     6181 ctcatgatga ttgtgtattc acttatcaat gtgccttatg catccttact cggcgtgatg
     6241 tcgcccgatc cgaaaatgcg caatgtactc tcttcttatc gaatgacatt cgcgttcatt
     6301 ggaagtttta ttacttttat gctgttgcag ccactggttg atttcttttc acaaacattt
     6361 gattctgcca gtctcgaaaa aaccggagag ggtctccaaa ctgcagttca tgcttcggtg
     6421 agcaattctc cggttggttg gactttaggc gtacttgtga taggtgccat ctgttctgtc
     6481 ctgtttttcc tgtgtttcag tcttacacgc gaacgtgtgg aagcagtcag caaagaagaa
     6541 aacgaaaata tatcggttaa acaggatctg cagcatttgc tacacaattc accctggtgg
     6601 attcttgtcg gtacggggct ggcggcactg ttattcaatg ccgtacgcga tggtgtggcc
     6661 atttattatt tccgcgatta tgtgggaagt aactacaccg cgccgttatt tggctgggat
     6721 atggctacca tttattttct tgttggtcag gcagccaata ttgtcggtgt cgtactggca
     6781 ccttcggttt cggcccgttt cggcaaacgc catacttata tgcttgccat ggttattgct
     6841 gccgtactga gtattgtgtt tttcttcatc cccaataatg taaccctgat attaatacta
     6901 caggtactca tttctgtttg cgcaggatat gtgttgccac tgctttggag catgttttcg
     6961 gacattgtcg attatcagga atacaaaaca aaccgccgtg catccggatt gattttttct
     7021 tcatcgtcca tgtcgcaaaa actgggctgg gcattgggag cagctcttgg aggctggata
     7081 ctggctattt tcaaatacaa tccggaaatc gtaacacaat tacctcaaac catatttggt
     7141 gaacggctca tgctgagtgt attccctgcc atttgtgcgc tgctggctgc ggtaggtatg
     7201 attctatacc cgctcactga agataaagtg aagcaaatag ctgaagaact gaataaaaga
     7261 cgtgaagatg caaagtaaca ctgcaagatt actcaaagag gctgaacaaa tattcactga
     7321 aaatatcagt cccttctggt tgtcgaaaat ggttgatgat gagcatggag gtttctatgg
     7381 tcgcataacc ggcgaaggtg agatttttcc cgatgcccca aaaggagccg tactgaacgc
     7441 ccgtatttta tggacatttg ctgcagctta ccgcattttc ggggaagaaa gttatctcat
     7501 tgctgccaga agagccaaag agtatctgct aaaacacttt tacgatatca ctgacggagg
     7561 tatttattgg atgctcgact ataccggaaa accccttgaa acaaaaaaac agatttatgc
     7621 acaggggttt gccatttacg gtttgagtga atactaccgg gctacatctg acaatgaagc
     7681 catcgaatgt gccaaagcga tattttttga catagaaaaa cacagctttg ataagctggg
     7741 aaacggttac ctcgaagcat ttactgaaaa ctggcaacct attgccgata tgcggttgag
     7801 cgaaaaggat gaaaatgtgg agaaaacgat gaatactcac ctccacattc tggagccata
     7861 taccaatctt taccgggtat ggaaaaatga aaagttgaaa catcaactcc aaaacctgat
     7921 tgaattattt ctggataaaa tcatcaaccc ggataattat cacctgaacc tgttctttga
     7981 tatggactgg acggacaagt ctgaaattat atcctacgga cacgacattg aagcctcatg
     8041 gttattgtac gaagcagcac ttgttcttgg tgatgaaaaa atccttaaaa gagtgaaaga
     8101 acttattcct catatcatag aggcttcact cgaaggattt cagccggata acagcatgat
     8161 ttatgaaaaa gaagaaaact ccggagatga agacagagac cgtcattggt gggttcaggc
     8221 cgaaacggtt gtgggtctgt tgaatcacta tcaattatat aacgacatca attccctcaa
     8281 caaagcaatc gcatgtttcg attatataaa aaacaacctg caggataaag aaaacggtga
     8341 ctggttctgg agcatccgca gcgacggaac tgtcaaccgc agcgacgaca aagcaggtat
     8401 ctggaaatgt ccctatcaca acagccggat gtgttttgaa atcatggaac gcttcggtcg
     8461 ggaataatac aagtgaaaca caaattttta cagaaattac taataccaat ctttcaaaat
     8521 gaaacacttt cttttaatca tgttatcgct ggtttccatt ttaacatttt catccttcaa
     8581 agcgccacgg aacactcctg tggctgttca cggcaatctg aaagttacag gaacacaact
     8641 tgtcgaccag caagatcaat ccgttatgtt gaggggcaca agttttggct ggcacaatct
     8701 ctggccgcgt tactacaaca aaaaagcagt atcctggctc gtcaaagact ggaaatgtaa
     8761 tgtgatacgt gcatcaatgg gtgttgccct tgacgattcg tatctcgaaa atcccgattt
     8821 tgcactgaaa tgtatgacca cagtgattga tgaagctata aaatgcggaa tttatgtgat
     8881 aattgatttc cacagtcata aaattcatac cgcagaagcc aaagaatttt tcaccttaat
     8941 ggcaggcaaa tacggtaaat atccgaatgt gatttacgaa atcttcaacg aacccgacta
     9001 ttattcctgg gaagaaatta aaacatattc ggaagaactg atctccacca tccgtactgt
     9061 cgatccggac aatatcattc tggttggttc gccacactgg gatcaggacc tgcatctggt
     9121 tgcagaaaat ccaatcaaag gacagaataa tattatgtac accatgcatt tctatgcagg
     9181 gacacacaaa aagtggttgc gcgaccgtac cgacgaagcg attgcaaccg gaattcccgt
     9241 tttcgtttcc gagtgtgccg gaatggaagc atcgggcgac ggacccattg atgatgaaga
     9301 atggaattcg tttctgagtt ggatggaaga aaaaaaaatc agctggattg tctggtcggt
     9361 ttccgataaa aatgaaacct gctccatgct ccttccccgt gcttcgtctg aaggcaactg
     9421 ggctgacgat gtgataaaaa agtggggtct cttaggccgc gatgccataa gaaaaggtaa
     9481 tcagaataac acaagcaatc attgaaacca caattcgctg taaaatagtt ttttacatta
     9541 attttatggc gtaaagaaac ataacaaatc acgtaaattt ataattttga acaacttata
     9601 ttccaatgaa aagtaaattt attgtattaa tcattctttc ggctttaacc tctgcggcat
     9661 ttgc
//
LOCUS       DAOPYB010000114         9489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_8500_length_9489_cov_10.210727, whole genome shotgun sequence.
ACCESSION   DAOPYB010000114 DAOPYB010000000
VERSION     DAOPYB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8500_length_9489_cov_10.210727"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..64)
                     /locus_tag="PKH58_09035"
     CDS             complement(<1..64)
                     /locus_tag="PKH58_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014299561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNX89214.1"
                     /translation="MRILILCTGNSCRSQMAHGFL"
     gene            complement(77..757)
                     /locus_tag="PKH58_09040"
     CDS             complement(77..757)
                     /locus_tag="PKH58_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX89215.1"
                     /translation="MTKILSVQNLSKKYDHRDGIVCALDDVSLSIEKGSFVSITGPSG
                     SGKTTLLLTLSGLIRPSSGKILFKEKDLSTASDKELAAFRKENVGFVMQNFSLIPYMT
                     AIQNVMIPLGLNGYSSAQKQALSSAVLELVGLENRLSHYPRELSAGQQQRVAIARALV
                     NKPSVVFADEPTGNLDPSLSSEILAFMQKINKELSITILMVTHSPQAANYGSVKINLV
                     DGKIDSQT"
     gene            complement(754..1971)
                     /locus_tag="PKH58_09045"
     CDS             complement(754..1971)
                     /locus_tag="PKH58_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNX89216.1"
                     /translation="MNLLKLVWLELGQRKSQLFSGILAITLGIGVIVAIRSISLVSEK
                     AVAVNLDNLGANILVLPQGAGVDNYYAADIDAPTFPEEYVERIVTSDLAGVDNLSPKL
                     TRRITINGQKLVLTGILPKNELASKPIWQNSGLFGSTLNAACGKNPSNETLGYKDEKL
                     QRKSIDSLGMADCIIGSNAAARLNIREGEKIIIDSTQLHVAKLLSETGTIDDDRIFAH
                     LHTVQNILGIHDQVSAIEIMGCCNAISDGLLSKLRNILPDTKVTTIGQIVSTQIKTNQ
                     LMNQISLIMLIIIVFVGSISIGNYLWANINERKKEIGILRMIGYPKSSIYYILILKAT
                     FMGLVGGVVGYLFGVMIAWFLGPYVAGINIVPVPILLLWSILLSILISLMGSLLPAWF
                     AARFEPYSNMQEV"
     gene            complement(1981..2721)
                     /locus_tag="PKH58_09050"
     CDS             complement(1981..2721)
                     /locus_tag="PKH58_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89217.1"
                     /translation="MKMTNRWLMMIMVMVFIVQPCFSQTKEKIDQANAKGKVVFLAVT
                     NGNVKLNEVRKVAQSAQKKFPASEVITFDKSDKSNAALVTKYGLLGAPVPIILVIAPN
                     GAVGGGFTLDKATPESLVKLIPTKKQATALLAFSEGKAAFIVLSKKSMKDKTPAVAEC
                     KKALSAIGGRGVIIDLDLGDKTESKFVDLLDPDMSATSSHIMVFNGKGQFTGEFSAPV
                     QSKDLVASSKKVVSSCCPSGSGAGCGKK"
     gene            complement(2741..3073)
                     /locus_tag="PKH58_09055"
     CDS             complement(2741..3073)
                     /locus_tag="PKH58_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX89218.1"
                     /translation="MIKKEVFDQDLQDLADFAKVISHPARLAILKYLAETKTCISGDI
                     SDQLPLSRTTVSQHLKELKRIGLIHGEIDGLKINYCICSRQIEQFLNRFESFFEPIKN
                     TVINCSTK"
     gene            complement(3378..3782)
                     /locus_tag="PKH58_09060"
     CDS             complement(3378..3782)
                     /locus_tag="PKH58_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX89219.1"
                     /translation="MITSYNPIYQEFNIEGVRHITPQNAFAEMQNETAIMVDVRERDE
                     YTLESIPLNNVFFFPLSSIMEHIQNVPDNKPLIVVCEDGVRSTKIVNLFNRYGFHDVA
                     NLDGGIVKWKQSGYPVEGVIPLGCGCGCSCSK"
     gene            complement(3797..4258)
                     /locus_tag="PKH58_09065"
     CDS             complement(3797..4258)
                     /locus_tag="PKH58_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX89220.1"
                     /translation="MINSARIFILFAGLSATIMLMSCMNKAGNDPLPRPEPPTRLSVQ
                     NMSNQVVSDTHKIVQTYKVTFIELGSVRCVPCRKMQPVMKSIEEKYGDQVKVVSHDVW
                     TKEGEPFGDKYGIKAIPAQVFLDEDGKEFFRHEGFFSEEELIKVLKSKGVK"
     gene            complement(4381..4833)
                     /locus_tag="PKH58_09070"
     CDS             complement(4381..4833)
                     /locus_tag="PKH58_09070"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNX89221.1"
                     /translation="MSELKVGLLSCSGEECLGGTISRLATRKVLEELKMVGTVTLCLP
                     LYLAGGEEERNFAKVFPTISVDGCDKLCAKRSTEKYSGKISGSIDVSKIIGNENALNT
                     KTVRNEDLEPEHFQMVDEVASAIVKAISCISPDSCSGKTFRGCSCGGE"
     gene            complement(4846..5238)
                     /locus_tag="PKH58_09075"
     CDS             complement(4846..5238)
                     /locus_tag="PKH58_09075"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNX89222.1"
                     /translation="MNTKVKVIPCSGIGKVFGLMAREAVLKTVHELCPDKSETVCLAY
                     IVTGDKEAKEKIEGCDCITVDGCPKMCASKNVLITGGIVKEAIKVLDTVKEHKGKQFG
                     TPTQLDADGKEVINEIAVKIAKKINEIS"
     gene            complement(5239..6087)
                     /locus_tag="PKH58_09080"
     CDS             complement(5239..6087)
                     /locus_tag="PKH58_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNX89223.1"
                     /translation="MVENYAILPCNGLDKCAGVISGEIAVKLCESGKNTIICPVFSKI
                     ADAKYRKITDEHPLLVIDGCATRCASKIAAEKNYRIFRKITVTEEAKRHHIELKDNLR
                     LLEDDNSLVEIIVDGFSDAGGMAAGVSEESGFSFEFEYETFQKDKFIFRVPRNTSVYF
                     NENDCWAYVIGNRARIGVTDFVQQNLSDILYFIPPEIGAIISQFDAAGEIESSKALVD
                     IISPVSGMVVSINEMLVQKPELINENPYESGWIAELELTDFESDKELLIGFDKYFELM
                     KKKVEG"
     gene            complement(6367..6747)
                     /locus_tag="PKH58_09085"
     CDS             complement(6367..6747)
                     /locus_tag="PKH58_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89224.1"
                     /translation="MSESEKEKPGFIMCVCTGKCPGFSQMDIWDFINRVRIELPVEYG
                     FIHPQLCEEDGDRFLNDYLKAHRKVVIGACALNMQRKMFKDAFAEAGLDINKDLTAID
                     IREMTTDQAVEKVSAALEKLEVQK"
     gene            complement(6965..7489)
                     /locus_tag="PKH58_09090"
     CDS             complement(6965..7489)
                     /locus_tag="PKH58_09090"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX89225.1"
                     /translation="MKKNVILLISILWIFIGSHAFSQESEGLLKRIPSVKIKDAKGNS
                     IDASSISNFGKPFIICFWKTCCKPPLQELDAISEVYEDWQDETGVVLYAVSVDDARTS
                     AQALPYANGQGYEFEILLDPNSDLKRAMNVNLIPSTFICDGKGNIIWQKTMYVPGDEN
                     EIFRMLKHAASTSE"
     gene            complement(7587..8045)
                     /locus_tag="PKH58_09095"
     CDS             complement(7587..8045)
                     /locus_tag="PKH58_09095"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNX89226.1"
                     /translation="MKSLLINIHSSLIMLIFAAMAFPAISDAQTNVASEAEIKIEVVY
                     FHAPNRCPSCVATENQTKSTLEKYFRAEMEKGVITFASLDLKEKKNESLVEKYEIVFP
                     TLLILKKQGNKEIKTDFSTSAFQYAYPEPKKYEKLLQAEILKQLNSNKIL"
     gene            complement(8061..8996)
                     /locus_tag="PKH58_09100"
     CDS             complement(8061..8996)
                     /locus_tag="PKH58_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89227.1"
                     /translation="MMKYRTIVAAFFVFAIPSVSSAQCCSAGNPSSYAFSDQTSLKAR
                     SLLISSSFKYGYSGKYFSGDQVSAMNLKTPASFSFIDLRASFGISQRFTVQTETGYYI
                     SKVQENPDPYPADRGKGLGDAAINVRYRFLKNLKHHLELNGSAGVRLPVGVFDQEKDG
                     VKLPLTIQPSSGSLVYLAGISAAKTLSNDKWRLFAVLSAEFPQLIDSRNFYYQYGNLY
                     NLSLAVVHPVHKLFTPALQLQGEMRSHATREAGQVVEASGYRVISLSPYIESAFAENW
                     IVNLYADIPVYKYFNGIQLANSFKAGIKLTRRISL"
     gene            complement(8993..9370)
                     /locus_tag="PKH58_09105"
     CDS             complement(8993..9370)
                     /locus_tag="PKH58_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89228.1"
                     /translation="MKWSNILIPLIVASVLSIAGLVSCSKEDENQLDNGDIKIISFTA
                     NDTVLKAWMDTATVVMTAKGQNLDYEWSCNHGTIHGSGDTVRYMAGECCVGLNTITCR
                     VFNDSSSVSASLNIRITSYYFPK"
BASE COUNT         2623 a         2116 c         1843 g         2907 t
ORIGIN      
        1 gcagaaatcc gtgggccatc tggctgcggc aactgtttcc ggtgcacagg ataaggattc
       61 tcatcgttcc acatatttaa gtttgtgaat ctatttttcc atccacaagg ttaattttta
      121 cacttccata attggcagcc tgggggctgt gtgtcaccat caggatagta atactcagtt
      181 ctttattaat cttctgcata aacgccagta tctcggacga taaggaggga tcaaggttac
      241 ctgtcggttc gtcggcaaaa acaacagaag gtttatttac caaggctctg gcaatagcga
      301 ctctttgttg ttgtccggcc gaaagctctc tgggataatg acttaacctg ttttcgaggc
      361 caaccagctc aagaactgcc gaggataacg cctgcttttg tgctgatgaa tatccgttca
      421 ggcccaacgg tatcattaca ttctggattg ccgtcatata aggaatgaga gaaaaatttt
      481 gcattacaaa accgacgttc tcttttctga acgcagccag ctctttatcg ctcgcagtcg
      541 acaaatcttt ctccttgaaa agtattttcc cggaggaagg acgaatcaaa ccacttaatg
      601 ttagcaataa cgttgttttg cctgaccctg acggacccgt tatggaaaca aaggatccct
      661 tttcgattga taaggacaca tcgtccaggg cgcacacgat gccatccctg tggtcgtatt
      721 ttttagaaag attttgtact gataatattt ttgtcatact tcctgcatat tagagtaagg
      781 ttcgaaacgg gctgcaaacc aagccgggag cagagagccc attaaagaaa tcaggattga
      841 gagtagtatc gaccaaagta agagtatggg aacaggtacg atgttaatgc cggcaacata
      901 aggtcccaaa aaccacgcaa tcataacacc gaacaggtaa ccgactacgc caccaacgag
      961 tcccatgaat gttgctttaa gtatgagtat gtaatagatg gaggatttcg gataaccaat
     1021 catccgcaaa ataccaatct cttttttgcg ttcatttatg ttggcccaca aataattacc
     1081 aatagaaatg ctgccaacaa aaacaatgat gatcagcatt atcaaagaaa tctgattcat
     1141 gagttgattg gtttttattt gcgttgaaac gatttgacca atggtagtca ccttggtatc
     1201 cggaaggata ttgcgaagtt tacttagtaa accatcggaa atagcattac agcaacccat
     1261 aatttcgata gcactaacct gatcatgaat accaaggata ttctgcacag tatgcaggtg
     1321 ggcgaaaatt ctgtcatcgt ctatggttcc tgtttcgcta agcaatttag ctacatgcag
     1381 ttgagtgctg tcaataataa ttttttctcc ttccctgatg tttaacctgg ctgcagcgtt
     1441 agatccgatg atacagtcag ccataccaag cgaatcaatt gatttgcgct gcagtttttc
     1501 atctttataa cccaatgttt catttgaagg gttcttacca caggcggcat tcagtgtgct
     1561 gccaaacaat cctgaattct gccatattgg ttttgacgcc aattcgtttt tgggaaggat
     1621 acctgtaagt acaagtttct gaccattgat agtgatgcgg cgtgtcagtt tgggagacag
     1681 attatcaact ccggccagat cggaagttac aatgcgttct acatactctt ccgggaaagt
     1741 gggagcatca atatcggcag catagtaatt atcgacccct gcaccctgag gcaataccag
     1801 aatattagcc ccgagattat ccaggttaac agccaccgct ttctccgaaa ccaatgatat
     1861 tgaccggatg gcaacaatta cgccaattcc taatgttata gcaaggatgc ccgaaaacaa
     1921 ctgggacttc cgttggccca attcaagcca tactaatttc agcaagttca tacagaaaca
     1981 ttatttttta ccacaacctg cacctgatcc tgaagggcag caactactca ccactttctt
     2041 tgaagatgca acaaggtctt ttgattgaac aggagcactg aattcgccag taaattgacc
     2101 tttaccgtta aataccatga tatgtgaaga tgtcgctgac atatcaggat ctaataaatc
     2161 aacaaatttt gattcagtct tatcgccaag gtcaagatca attataacgc ccctgccacc
     2221 tatagccgac aaggcttttt tacattcagc gacggctggt gtcttatcct tcatagattt
     2281 ctttgataaa acaatgaatg cggctttacc ttcgctaaaa gctaataacg ccgtagcttg
     2341 ttttttagtc gggatgagtt ttaccagact ctcgggagta gccttatcaa gagtgaaacc
     2401 gccgccaact gctccattcg gggcaatcac caaaatgatg ggcacaggag cccccaacaa
     2461 gccatacttt gtgacaaggg cagcattcga tttgtccgat ttatcgaatg ttattacctc
     2521 cgaagcgggg aatttctttt gagcgctttg ggccactttc ctgacttcat taagcttaac
     2581 attgccattg gtcaccgcca gaaaaacgac tttcccttta gcattagcct ggtcgatttt
     2641 ttctttagtc tgagaaaaac atggttgcac gatgaaaacc ataaccatga tcatcatcag
     2701 ccatcgattg gtcattttca ttttacgggt tattagtaaa ttacttggta gaacaattaa
     2761 tgacggtgtt ctttatgggt tcaaaaaaag actcgaatct gttcaggaat tgttcaatct
     2821 gtctggaaca gatacaataa tttattttaa ggccatctat ttcgccgtgt atcagtccaa
     2881 tccgttttaa ttctttcagg tgttgcgata cagtagtccg gctgagcggg agctgatcag
     2941 aaatgtcgcc tgaaatgcag gtttttgttt cagccagata ttttaaaatc gcaaggcgcg
     3001 caggatgaga aatcaccttt gcaaaatcag ccagatcctg aaggtcctga tcgaaaactt
     3061 ctttcttaat cataggtatt gttatatgtc gcaaacatac gacattattt tgaaatcaaa
     3121 gtttatcagg aaaaattttt ctcataccga tttaaacttt catctgcagt ttaatttcac
     3181 ggctaaatca ttattcttct ttcagttaat catcattgtt aaattccctt acaacgaatg
     3241 aactgaaaca tcccattaat ttgagaatgc ctgcaatagt attccggtaa ttccgccgaa
     3301 tacgaaaaag ccaatagatt ttacctgcat tatggcagat ttataaggct aaatattccg
     3361 tgaaacttaa ttggttttta cttcgaacaa gagcagccac agccgcaacc caatggaatg
     3421 acaccttcaa caggatagcc ggattgtttc cacttgacaa ttcccccgtc tagatttgca
     3481 acatcatgga atccataccg gttaaataaa tttacaattt ttgtgcttct tacaccatcc
     3541 tcgcaaacaa cgattaaagg tttattgtca ggaacatttt ggatatgttc catgatggag
     3601 gagagtggaa agaagaaaac attattcaga ggtattgatt caagcgtgta ttcatctctc
     3661 tcgcgtacat caaccatgat ggctgtttcg ttttgcattt ctgcaaaggc attctgaggt
     3721 gtaatgtgcc gcacaccttc aatgttaaat tcctgatata ttggattata cgatgtgatc
     3781 atagtttacg gataatttac tttactcctt tgctttttag cacttttatc aactcttcct
     3841 cagagaaaaa cccttcgtga cgaaagaatt cttttccatc ttcatctaaa aacacttgag
     3901 cgggtatagc tttaatacca tacttgtcgc cgaaaggttc cccttctttg gtccatacat
     3961 cgtgagacac cacttttacc tggtcaccgt acttctcttc gatggatttc atgacaggtt
     4021 gcattttcct gcaaggtaca caacggaccg aacccaattc aatgaaggtg actttataag
     4081 tttgaacgat cttatgggta tccgaaacaa cctgattgct catattttga accgacaaac
     4141 gtgtcggcgg ttctggtcgg ggcagcggat cgttcccggc tttattcatg caactcatca
     4201 acattattgt agccgataag ccagcaaaaa gtataaaaat gcgcgctgaa tttatcatag
     4261 tttatgtgct ctttattttt gatggaacta actcctaaag cgtcagcttt aatcagtcca
     4321 ccctggctcc ggatgattca ttaaaaaagg gattatctgc atgatatcat cctcaatctc
     4381 ttattctccg ccacagctac agccacgaaa cgtttttccg gaacaggaat ccggagatat
     4441 gcagctaatc gcttttacaa tcgcgctggc tacttcatct accatctgaa aatgttcggg
     4501 ttcaagatct tcattcctca cggttttcgt gtttagcgca ttttcattcc ctatgatttt
     4561 cgatacatct attgaaccgc tgattttacc gctgtatttt tcagtacttc gtttagcgca
     4621 aagcttatcg cagccgtcaa ccgatattgt cggaaacact tttgcaaagt ttctttcttc
     4681 ttcgccacct gccaggtaca acggcagaca cagtgttacc gttccgacca ttttaagttc
     4741 ctcaaggact tttcgggtgg ccagcctcga aattgtaccg ccgaggcact cctcgccgct
     4801 gcatgataac agacctactt tcagttcaga cattgatgct cctccttaac taatttcatt
     4861 tatctttttc gcgatcttca cagcaatttc attgattact tcttttccgt cggcatctag
     4921 ctgtgtgggt gtaccgaact gtttaccttt atgttctttc actgtatcca gcacttttat
     4981 agcttctttt actatacctc cggtgatcag gacgtttttt gaagcacaca ttttcggaca
     5041 accgtcaacg gtaatgcaat cgcagccttc aattttttcc tttgcttctt tatcaccggt
     5101 tacaatgtag gcaaggcaca ccgtttccga tttatccgga cataattcat ggacagtttt
     5161 caataccgcc tctcttgcca tcaatccgaa caccttacca atgccactgc aaggaataac
     5221 tttcactttc gtattcattt aaccctccac cttttttttc attaattcaa aatacttatc
     5281 gaatccgatt aaaagttctt tatcgctttc aaaatcagtc agttcaagtt cggcaatcca
     5341 acccgattca tagggattct cgttgataag ttcaggcttt tgtacaagca tttcatttat
     5401 agaaacgacc ataccgctta ccggtgaaat aatatctacc agagcctttg acgattcaat
     5461 ctcacctgct gcatcgaact ggctgattat tgccccaatt tcaggcggta taaaatataa
     5521 gatatcggaa agattttgtt gaacgaaatc cgtaacgccg attctggcgc gattcccgat
     5581 cacataagcc cagcaatcat tttcgttgaa gtaaacagaa gtatttctcg gaaccctgaa
     5641 gataaacttg tccttttgaa aggtctcata ttcgaattca aatgaaaaac cggattcctc
     5701 ggaaactccg gcagccatgc ctccggcatc actaaaaccg tcaactataa tttcaaccag
     5761 agaattatca tcttccagaa gcctgagatt atcctttaat tcgatatgat gccttttagc
     5821 ttcttcggtt actgttattt tcctgaatat cctgtagttt ttttccgcag cgatcttact
     5881 tgcacagcgg gtagcacacc catctatgac caacagcgga tgttcatcgg ttatttttct
     5941 gtatttggca tcagcaattt tggaaaagac gggacaaatg atcgtattct taccgctttc
     6001 acataatttc actgctattt cccctgaaat tactcctgcg catttatcca atccattgca
     6061 cggaagtatg gcataatttt caaccattgt tcccctcctt tctcatttcc ttaatctttt
     6121 gtgtcgtcgc atttttgtcg ggaaaggtaa ttgcatcggg gccgcaggtc ttggagcatg
     6181 ccctgcaaaa aacaacgcag ttattaggat tcctgacgat caacctgatt tcatcatttt
     6241 cattgacctc aaaaacctta tggggacaaa atttcacgca ttccatgcaa aaattgcatt
     6301 taccgtaatc gatagcaggt ttccaatcga tcttatcacg ttcaactccc ataaatgtag
     6361 tttcgctcat ttctgtacct ccagtttttc aagtgctgca ctcacttttt caactgcctg
     6421 atcggtcgtc atctcgcgta tatcaatagc tgttaaatct ttatttatat caagaccggc
     6481 ttctgcaaaa gcgtctttga acatcttacg ctgcatattg agggcgcaag ccccgatgac
     6541 gactttccgg tgagctttaa gataatcatt caaaaaacga tctccatctt cctcgcaaag
     6601 ctgcggatgg ataaatccat actcaacagg caattcgatt ctcaccctgt taataaagtc
     6661 ccatatatcc atttgtgaga atccgggaca ttttcctgtg catacgcaca taataaaacc
     6721 aggcttttcc ttttctgatt ctgacatgat taatttttta agcagtccgt tttattcaat
     6781 ttattgtttt tcaaagcacc tgagttttgc cagagcattt ttaccgcgaa aaactgaggt
     6841 tgtttttctc actcagcgac taattcttta atcagatcca tcgggattat gatttcatca
     6901 ggaacttgat ttcaagacaa tttgatttgc attaaagtcc aaatggatat ctccgctttt
     6961 ccccctattc tgatgttgag gcagcatgct tcagcatcct gaaaatctcg ttttcatcac
     7021 cagggacata catcgttttc tgccagatta tgttaccttt cccgtcgcag atgaaggttg
     7081 acggtatcag attcacattc atggctcgct tcaggtcgga gttgggatcg agaaggattt
     7141 cgaactcgta tccctgcccg tttgcataag gtaaagcctg ggcggatgtt cgggcatcat
     7201 cgacagaaac agcatataaa acaactccgg tttcgtcctg ccaatcctca taaacctcgg
     7261 agatggcatc aagttcctgc agcggcggtt tacaacaggt tttccagaaa caaatgataa
     7321 atggcttgcc gaaatttgag attgatgaag catcaattga gttccccttt gcatctttta
     7381 tttttacgga tggtatacgt ttcaacagcc cttctgattc ctgagaaaat gcatgtgatc
     7441 cgataaatat ccataatatg ctgatgagga gtatcacatt ctttttcatc tgtaagggta
     7501 ttaaagtttc tctttgatta attatttagt atttcgtaaa aatacgaaac gtatttatgg
     7561 tattgcattt agcttagcat tttttcttaa agaattttat tgctattaag ctgtttcaga
     7621 atttcagcct gcagaagctt ttcatatttt ttaggctcag gataggcgta ctgaaatgct
     7681 gatgtggaaa aatcggtctt aatttcctta ttcccttgtt tttttagaat cagtaatgtt
     7741 gggaaaacga tttcatactt ttcaacaagt gattcgttct ttttttcttt caggtcgagt
     7801 gaagcaaacg ttatcacacc tttttccatt tccgctctga agtatttttc aagagtggat
     7861 tttgtttggt tttcagttgc aacacacgac gggcaacggt tgggtgcatg gaaatagacc
     7921 acctcaattt taatctctgc ttccgatgcc acattagttt gagcatcaga aatggcagga
     7981 aatgccattg ctgcaaaaat cagcatgatc aaggatgaat gaatattaat caacaatgat
     8041 ttcatatcat gtgctttaca tcaaagggag attctgcggg ttagttttat gccggcttta
     8101 aaagagtttg caagttgtat accattaaag tacttgtaaa caggtatatc ggcatataga
     8161 tttacgatcc agttttcggc aaaagctgac tcgatatacg gagacaggga gatgaccctg
     8221 tagcctgatg cttccactac ctgacctgct tcacgggtgg catggctgcg catttcgccc
     8281 tgaagttgca gagcgggagt aaacaactta tggacaggat gaacaactgc cagtgacagg
     8341 ttatacaggt taccgtattg gtagtaaaag tttctggaat cgatgagctg agggaattcg
     8401 gcagacaaca ctgcgaacaa tctccatttg tcattgctta gcgtttttgc cgctgatatt
     8461 cccgcgagat aaacgaggct gccggaggat ggctggatgg ttaaaggaag tttgacgccg
     8521 tctttttcct gatcgaatac tccgacaggg agcctcaccc cggctgaacc attcagctca
     8581 aggtgatgtt tcagattttt aaggaaccga taacgcacat tgatggctgc atcgccgagg
     8641 cctttcccac gatcggcagg ataaggatcc ggattttcct gtaccttact gatataataa
     8701 cccgtttcgg tttgtacggt aaaacgttgc gatatcccaa aggatgctct caagtcgata
     8761 aacgaaaagc tcgcaggtgt tttcaaattc atcgcagata cctggtcacc cgaaaagtac
     8821 tttccggagt aaccatattt aaacgatgaa gaaataagca acgaccttgc tttaagcgaa
     8881 gtctgatcgc tgaaagcata agaagatgga ttgccggcgc tgcagcattg tgctgatgat
     8941 accgacggta ttgcaaatac gaaaaaagcg gcaacgatag tacggtattt catcatttcg
     9001 gaaagtaata tgaggttatt ctgatattaa gagaggccga tacactgctc gaatcattaa
     9061 aaaccctgca tgttatggta ttaaggccca cacaacactc gccagccatg taacgcactg
     9121 tatcgccgct gccatgaatg gtgccgtggt tgcaggacca ctcataatcc aggttttgcc
     9181 ctttggccgt cataacaaca gtagccgtat ccatccaggc tttaagcacg gtatcattgg
     9241 cagtgaaact gatgatttta atatcgccgt tatccaactg attttcatct tcctttgagc
     9301 aggaaacaag acctgctatg gataatactg aagcaactat taacggaatc aggatgtttg
     9361 accacttcat tttaaagtaa ttaggttagg ttgttcttga tctcatagta cagggacatt
     9421 gtccggtcat cgactcggca ctctgcttca tcggcaaccc gtcgcgttcc cattcggcaa
     9481 ttccgccgg
//
LOCUS       DAOPYB010000115         9353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_8670_length_9353_cov_10.250269, whole genome shotgun sequence.
ACCESSION   DAOPYB010000115 DAOPYB010000000
VERSION     DAOPYB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8670_length_9353_cov_10.250269"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1499)
                     /locus_tag="PKH58_09110"
     CDS             complement(96..1499)
                     /locus_tag="PKH58_09110"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNX89229.1"
                     /translation="MNKKLLRLKYIFFDIVAAFIVWVLFMLFRRIVNDGEVFQGLDIF
                     IPVYNFYTNLFLFPVFSVVIHYLTGYYENPIKESKTLEFFTTLVASATISIIIFFVLL
                     LDDIVISYLNYYYSLTVLFLLLFFITLIFRTLLSWRVRRNFYRKKWTKKTLVIGTGAN
                     AQKIASGIERNQLFNTITGFIQVDSAEKSVEPKMIVGNLQNLKEFIEKQDVKEVIVAL
                     DDADEHKIFKIISNLFQYDIDIRFTPRQYEIVTGKARINHYGINPLVSINNTTMNDWE
                     VSVKRTFDIIISAVALILLLPLMIYIGLKIKIDSKGPVLYRQKRIGRNGVEFEILKFR
                     TMFTGSENGKPKLSSANDERITNTGRTLRKYRLDEIPQFWNVLKGDMSIVGPRPERKF
                     FIDQIVKEAPYYCLLYRIRPGLTSWGPIKIGYSDTLEKMIERLDYDIIYMENMSLLTD
                     IKILVSTLEILFRGKGI"
     gene            complement(1506..2069)
                     /locus_tag="PKH58_09115"
     CDS             complement(1506..2069)
                     /locus_tag="PKH58_09115"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNX89230.1"
                     /translation="MIVEIKKTIEVLRNGGVILYPTDTIWGLGCDATNPDAVQKVYEI
                     KKRAETKSMLVLVDSPAKLPAYVDEIPDMAWDLIELTTKPLTIIYPGAKNLASNLISA
                     DKTIGIRVTSEAFSKKLCEMFRKPIVSTSANISGEKSPAFFSEITDEVKNAVDYIVNI
                     RQDDTTPANASSIIKLGIGNQIEIIRQ"
     gene            2233..3435
                     /locus_tag="PKH58_09120"
     CDS             2233..3435
                     /locus_tag="PKH58_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNX89231.1"
                     /translation="MTQSPKENHRLFPEECLIREITGKPRLSIGIPKENPDIETRLAL
                     TPEGVAIITEEGHSVYVQRGAGEPMSYTDLQYSEAGAFMVDDMSEVYGADIVLKITPP
                     TIEELNLMHNRSTIFSMLQLSNMSKECIELIMLKKMNAIAYELIKDEQKAFPVVNSIS
                     EIEGNTAIAVISDLMSSERGGKGLLLGGVAGITPPEVLIIGASITGSVAARTALALGA
                     QVKVFDHDINKLRKIQHYLGVQIFTSVIHPAVLFRALASADAVIGNLRYINGSERFMV
                     SEDLVKTMKKGAVIVDLSVDQGGCFETSECRTITNPVFVKHGITHYCVPNISARVSRT
                     SSMALSNIFSPILLKIGHSGSVKQSIAESSGFRHGAYVFNGIMVNRLIGDYYNLPSND
                     IGLLLAGY"
     gene            3534..5780
                     /locus_tag="PKH58_09125"
     CDS             3534..5780
                     /locus_tag="PKH58_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNX89232.1"
                     /translation="MSDQKETVQTAEDAIIQKEFDELLQDYLKTNHRKKVEIITKAFN
                     FACAAHKGVKRRSGEPYILHPLAVARIVVSEIGLGSTSICAALLHDVVEDTDYTTEDI
                     ENLFGPKIAQIVDGLTKIAGGVFGEKASEQAENFRKLLLTMSEDIRVILIKIADRLHN
                     MRTLGSMPPAKQYKIAGETEYIYAPLAHRLGLFRIKTELENLSFKYEHPETYLEIEQK
                     LEGAKKSLNKFYNEFSKPIYNKLIELGYEFELKERIKSVFSVWNKMSTRNIQFEDVYD
                     ILALRIIFKPKESLNEKDQCWMLYSAITAIYKPHPERIRDWVSTPKANGYESLHVTVM
                     APGGRWVEIQIRSERMNEIAERGLAAHWKYKTGEDDISELDKWLKTIKELLENPEPNA
                     MDFIDTFKLNLFASEIFVFTPKGEIKTIAKDATALDFAFMLHSDVGYRSIAAKVNHKL
                     VPLNHKLSSGDQIEILTSKKQVPQAEWLEFVTTAHAKSKLRQYYKKEEKNLISNGQKK
                     IEAALEAIQLKPANENIVKLLNYYNLETRSDLYLQAGRDLINMDEISKTIFKPRTQSV
                     FSKILKNPFGGSSSIDKKSPEIKKEEDTHKFDFKKTYKLNDMDAGKSYELASCCHPIP
                     GDDVLGYVTEQKMIQVHKRQCSVAAVIKSNFGNRIVNVEWGQHKALFAETLEIKGIDK
                     VGIVIEILKVISEGYNVNISKINIESNAGVFLGLFEVIIHDTNDINNLINNLIKIKEI
                     NSVQRIQK"
     gene            5882..6796
                     /locus_tag="PKH58_09130"
     CDS             5882..6796
                     /locus_tag="PKH58_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX89233.1"
                     /translation="MQLTKKHGHIALFGANLIFGLNTPISRTIIPEIIEPFSLTFLRL
                     AGSTLLFWIISFFVKKEKVPFKDIVMLFFASIFGIVLNQIPYITGLSMTSPIDASLVT
                     SLLPIITMLLAALIIKEPVTWLKAIGVLVGASGAFILIMSSANHNGKGSFTGDIMILM
                     AASSYALYLTLFKNLISKYSSVTVMKWMFLFASLLSYPVLHKSVAQTDFTSLDTSVWL
                     RIGYVVVFATFISYLLIPIGQKTIRPTTLSMYNYMQPVVASLVAVGLGMDTFGYEKIV
                     STVLVFAGVYIVTQSKSRAQMELEKIRK"
     gene            7079..9133
                     /gene="metG"
                     /locus_tag="PKH58_09135"
     CDS             7079..9133
                     /gene="metG"
                     /locus_tag="PKH58_09135"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNX89234.1"
                     /translation="MTFKRTTVTSALPYANGPVHIGHLAGVYVPADIYVRYLRLKGEE
                     VIFIGGSDEHGVPITIKARKEGVSSQDIVDRYHTIIKESFEEFGISFDIYSRTTSKTH
                     HQLASDFFRKLYDEGKFIEQTTEQYYDTEAQQFLADRYITGTCPHCGNENAYGDQCEA
                     CGTSLNATDLINPKSAISGSIPEMRDTKHWYLPLDQYEPWLREWILENHKEWRPNVYG
                     QCKSWLDMGLQPRAVSRDLDWGIPVPVEGAKGKVLYVWFDAPIGYISNTKELCDAQPE
                     KFGSWETWWKDPETRLVHFIGKDNIVFHCIVFPSMLKADGSYILPDNVPSNEFLNLES
                     DKISTSRNWAVWLHEYLEDFPGKQDVLRYVLTANAPETKDNDFTWKDFQARNNNELVA
                     IYGNFINRALVLTQKYFGGKVPPIGTKTDYDIQTISDFSKVKTELENQLDNFHFREAQ
                     KEAMNLARIGNKYLAETEPWKLAKTDMDRVATILNISLQIAANLSIAFEPFLPFSSEK
                     LRKMLSMSEFDWNELGRTDLLAEGLQLGTPELLFEKIEDEQVDAQVQKLENTKKANEI
                     SSYKPKPIKETVSYDDFGKLDIRVGTVLECTKVPKADKLLQFRIDDGMGGRTILSGIA
                     QSYPNPEELVGTQVCFIANFEPKKLRGVMSEGMILSAENSDGKLVLVQPKDIVRNGVN
                     VG"
BASE COUNT         2930 a         1963 c         1880 g         2580 t
ORIGIN      
        1 ttcggataat tacaaactca cttcctgatg taataacagg taatcttatc gcgggtaaca
       61 aataggaatg ctttgatctt acatgctatg gtttgtcata tacctttacc tctgaaaaga
      121 atttcgagcg tcgagaccag tatcttaata tcggtcagca gcgacatatt ttccatgtag
      181 attatgtcat aatccaatcg ttcaatcatc ttttccagtg tatccgaata gccgattttg
      241 atgggtcccc acgaagtcag tcccggtctg atccgataga gcaaacaata gtagggcgct
      301 tcctttacaa tctgatcaat aaaaaatttt ctttcgggtc gaggtccgac aatgctcata
      361 tcacctttca gcacattcca gaattgtggt atttcatcca gcctgtattt tcgtagtgta
      421 cgtcccgtat ttgtaattcg ttcgtcattg gcgctgctta gtttgggttt gccgttttcg
      481 gatccggtaa acatggttct gaatttcaaa atttcaaatt ctaccccgtt tcggccaatc
      541 cttttttgtc ggtacaatac cgggcctttt gaatctatct ttattttcag gccgatataa
      601 atcatcaggg gtagaagtaa aatgagagca acagccgaaa taattatgtc aaaggtcctt
      661 ttaacactga cctcccaatc attcattgtt gtattgttga tactcaccaa cggattaatg
      721 ccgtaatgat taattcgtgc ttttccggtt acaatttcat attgccgggg tgtaaaccgt
      781 atatcaatat catactggaa caggttactg ataattttaa agattttatg ttcatcggca
      841 tcatcaagtg cgacaatcac ttcctttaca tcctgtttct cgataaattc tttcagattt
      901 tgcaagtttc caacaatcat tttcggctca acactttttt ccgcggaatc aacctgaata
      961 aaccctgtga ttgtattaaa cagctgatta cgttctattc ccgaagctat tttttgtgcg
     1021 ttagcacccg taccaatgac aagcgtcttt ttggtccatt tttttcggta aaaattccgt
     1081 ctcactctcc aactcagcag cgttctgaaa atcaacgtta taaagaacag aagcaaaaac
     1141 aaaacagtaa gtgaatagta ataattaaga taactaatga ctatatcatc caacaaaaga
     1201 acaaaaaaga taataataga aattgtggct gaagcaacca gggtagtaaa aaattcgaga
     1261 gttttggatt cctttatcgg attctcgtaa taccctgtca ggtaatgaat aacaacacta
     1321 aaaacaggaa acagaaaaag attggtataa aaattataaa ccggaataaa gatatccagt
     1381 ccctgaaaaa cctctccatc gttaacaatt cttcgaaaaa gcataaaaag cacccatact
     1441 atgaatgccg caacaatgtc gaaaaaaata tattttaacc tgagtagttt tttattcatg
     1501 tcaacctatt gacggattat ctcaatttga tttccaatgc cgagtttaat aatcgaactt
     1561 gcattagccg gagttgtatc atcctgccgg atatttacaa tgtagtcaac tgcattttta
     1621 acttcgtcgg taatttccga aaaaaatgcc ggtgattttt ccccgcttat atttgccgaa
     1681 gtggaaacaa tcggtttgcg aaacatttca cataattttt tcgaaaaagc ttctgaagta
     1741 accctgatac ctattgtctt atcagcactt atcaaattcg aagccagatt ttttgctccc
     1801 ggataaatga tggttaacgg cttggtggtc agttctatca ggtcccatgc catatcgggg
     1861 atttcatcga catacgcagg cagctttgcg ggagaatcca ccaaaaccag catagatttg
     1921 gtttccgcac gtttttttat ttcgtaaact ttctgaaccg catccggatt tgttgcatcg
     1981 caaccgagcc cccagattgt gtcggtcgga tataaaataa cccctccatt ccgaagaact
     2041 tcaatcgttt tttttatttc tactatcata aggaaaagta acctgattga taacgaacat
     2101 ctgtctgatt tattacatga atcagtttac aaagataatt aaatatcggt aaacgaagga
     2161 aaattattag ctgcacaatt tgttcaattc aataatagta attagatttg tggctataaa
     2221 aaaactaatt ccatgacaca atccccaaaa gaaaatcaca gattattccc tgaagagtgt
     2281 ctgatcagag aaataaccgg gaaaccccgt ctgtcgattg ggattccgaa agaaaatccg
     2341 gatatagaga cacgactggc gttgactccc gagggtgtag caatcattac tgaagaaggt
     2401 catagcgtgt acgttcagcg gggagcaggc gaaccaatgt catataccga tcttcaatac
     2461 agcgaagcag gtgcgtttat ggtcgatgat atgtctgaag tttacggcgc tgatattgtt
     2521 ctgaagatta caccgcccac aattgaagag ttaaatctga tgcacaatcg ttccaccatt
     2581 ttctccatgt tgcaactgag taatatgtcg aaagaatgca ttgaactgat tatgctaaaa
     2641 aagatgaatg ctattgccta tgagttgatt aaggatgaac aaaaagcgtt tcctgtagtg
     2701 aattcgatct ccgaaataga aggcaatacc gccatcgctg taatttccga tctgatgagc
     2761 agcgaacgtg gcggcaaagg gttattgctg ggaggtgtag ccggaattac cccacccgaa
     2821 gtcctgataa tcggggcaag cattaccggc tcggtggcag cacgtactgc attggcactg
     2881 ggagctcagg taaaagtttt tgaccacgat atcaataaat tacgaaaaat acaacattat
     2941 ctgggtgtcc agatattcac atctgtcatt catcctgccg ttttattcag ggcactggca
     3001 tcagccgatg ctgttatcgg aaatctcaga tatataaatg gttcagagcg atttatggtt
     3061 tcggaagatt tggtaaagac gatgaaaaaa ggagctgtaa tcgtggattt gagtgtcgat
     3121 cagggcggat gttttgaaac atcggaatgc cgaacgatta ccaatccggt atttgtcaaa
     3181 cacgggataa cgcactactg cgtacccaac atttccgcac gggtaagcag gacttcgtcc
     3241 atggcgctga gcaatatttt tagtcctatt ctacttaaaa taggccattc gggcagtgta
     3301 aagcaatcca tagcagaaag ttctggtttc cgtcacggtg cttatgtttt caacggtatc
     3361 atggtcaaca gactaatcgg cgattattac aatttaccat caaacgacat cggactgctg
     3421 ctggcaggtt attgattttt ttatatagga aaaatcagta tttgctcaac cctgtatcga
     3481 ggttttcagc aggaacaaaa aaattataga gaacgagttt aggaaaagga aacatgtcag
     3541 accaaaaaga aacagtgcag acagccgaag acgctataat tcaaaaggaa ttcgatgagt
     3601 tgttgcagga ttacctgaaa accaaccacc ggaagaaagt tgaaatcatc acaaaggctt
     3661 ttaacttcgc ctgtgcagcg cacaagggag taaaacgccg ttcgggtgaa ccatatattc
     3721 ttcacccact ggcagtggcc cgaattgtgg taagcgaaat cggacttggt tccacttcca
     3781 tttgtgctgc actgttgcat gatgttgtcg aagacacgga ttataccacc gaggatatcg
     3841 aaaatctttt tggacctaaa atagcgcaaa tcgttgatgg tctgacaaaa attgccggag
     3901 gagtattcgg cgaaaaagca tctgaacaag ctgaaaactt ccgcaaactt ctgctgacca
     3961 tgtcagaaga tatacgggta atactcataa aaatagccga ccgccttcac aatatgcgaa
     4021 cgctgggctc tatgccacct gctaagcaat ataagattgc cggtgaaaca gaatatattt
     4081 atgcaccgct tgcccatcgt ctgggccttt tccggataaa aacggaactc gaaaacctta
     4141 gcttcaagta cgagcatccc gaaacatatc ttgaaataga gcagaaactg gaaggtgcaa
     4201 aaaagtcgct gaataaattc tacaacgaat tttccaaacc aatttacaac aaactaatag
     4261 aactgggtta tgaatttgag ttgaaggaaa gaataaaatc tgttttttcc gtttggaaca
     4321 agatgtcgac ccgcaacatt cagtttgagg atgtatatga cattctggca ctgcgcatca
     4381 tcttcaagcc caaagaaagt ctgaacgaga aagaccagtg ctggatgctt tattcagcca
     4441 taacagccat ctacaagcca catccggaac gcatccgcga ctgggtgagc actccgaaag
     4501 ctaacggtta cgagtcgctc catgtaacgg tgatggctcc cggcgggcgc tgggtggaaa
     4561 tccagattag aagtgaacgc atgaatgaaa ttgcagaacg ggggctggcg gcacactgga
     4621 aatacaaaac cggggaagat gatatctcgg aactcgataa atggctgaaa accataaaag
     4681 aattgctcga aaatccggag ccgaacgcga tggatttcat cgataccttc aaactgaacc
     4741 tctttgcatc cgagatattt gtttttacac cgaagggcga aattaaaacg attgccaagg
     4801 atgccacagc acttgacttt gccttcatgc ttcactccga cgtaggttac cgaagcattg
     4861 ctgccaaagt taatcataaa ctcgttccgc tcaaccacaa actcagtagt ggtgaccaga
     4921 ttgaaattct tacttccaaa aaacaggttc cgcaggctga gtggttggaa tttgtcacaa
     4981 cagctcatgc caaatcaaaa ctgaggcaat actacaaaaa agaagaaaaa aatctgattt
     5041 caaacggaca gaaaaaaatt gaagctgcgc tggaagctat tcagttgaaa ccggcaaatg
     5101 aaaatatcgt aaaacttcta aactactata acctggaaac cagatccgac ttatacctac
     5161 aggccggacg tgatctgatt aatatggatg agatctcaaa aaccattttc aaacccagga
     5221 cacaaagtgt tttttcgaaa atattaaaaa atccctttgg aggcagtagt tctatcgata
     5281 aaaaatctcc ggaaatcaaa aaagaagagg atactcataa atttgatttt aagaaaacgt
     5341 ataagctgaa tgacatggat gccggtaaat cctacgaact cgcatcgtgt tgtcatccta
     5401 tacccggcga tgatgtactg ggttacgtta cggagcaaaa aatgatacag gttcacaaaa
     5461 gacaatgctc ggttgctgct gtgattaaga gcaattttgg taatcgcatt gtaaatgtgg
     5521 aatggggtca gcataaagcc ctttttgctg aaaccctgga aattaaaggg atcgacaagg
     5581 tgggtattgt cattgaaata ttaaaggtaa tttcggaagg atataatgtg aatatcagta
     5641 aaatcaacat tgaaagcaat gccggtgttt ttttaggact gtttgaagtg attatccacg
     5701 acacaaatga cattaacaat ctgataaaca atctgataaa gattaaggaa attaattcag
     5761 tacagcgtat tcaaaaataa ttttcgcatt cccgggatgt tctccctgat tttgagtaat
     5821 tttgcacctt gtttttcttt aaccacaggc atttctacca atgcctcatg cagatattca
     5881 tatgcaactt actaaaaagc acgggcacat cgctttattc ggcgccaatc tcattttcgg
     5941 actaaacaca cccatttccc gcactataat acccgaaatc attgaacctt tctctctcac
     6001 ttttctgcgc ttagccggat caaccctttt attctggata atatcttttt ttgtaaaaaa
     6061 agagaaggtt cctttcaaag acattgtgat gcttttcttt gcttcgattt tcggaattgt
     6121 actcaatcag ataccgtaca ttaccggttt atccatgact tcacctatcg atgcctcgtt
     6181 ggtcaccagt ttgctgccaa tcattaccat gctgttggca gcactgataa tcaaagaacc
     6241 ggttacctgg ctaaaagcta tcggcgttct ggttggagct tcgggagcct ttatccttat
     6301 tatgagcagt gcaaatcaca acggaaaagg aagttttacc ggtgacataa tgatcctgat
     6361 ggcagcttcc tcctatgcct tatacctaac tctcttcaaa aacctgattt caaaatattc
     6421 atctgttaca gttatgaaat ggatgtttct gtttgcatcc ctcttatcct atcctgtttt
     6481 gcataaatca gtagctcaaa ccgatttcac ttcactggac acaagtgttt ggttgagaat
     6541 aggttacgta gtggtatttg ccacattcat atcctattta ttaattccga tagggcagaa
     6601 aaccatccgc cctacaacgc tgagtatgta taattatatg cagccggttg tagcttcgct
     6661 ggttgctgtg gggctgggta tggacacttt tggatacgaa aagatcgttt ccaccgtact
     6721 tgtatttgcc ggagtttaca tcgtaactca gagtaaatca agggcacaaa tggaattgga
     6781 aaaaatcaga aaataaggtt aaatagaaat catatttcat ttagttcggc aagaattgtt
     6841 gtcttttcat aacttaccgg ttcagcactt tcaggaaaga atgctatttt cctttttcaa
     6901 aatgaccgtt agtttatttt ttcaaaaaac atgaaaaata aatcataata aaattgctat
     6961 aaaaagcagc ctgtaaggtc ttattgaatt agcagttttc ttattatttt ttaattaatt
     7021 ctccgtatct ttgcacctca aaaaaacgaa cgctaaatca ttaaataaga acccgcaaat
     7081 gacttttaaa cgtaccactg taacttctgc cctgccctac gccaatggtc ccgtgcatat
     7141 cggacatcta gccggcgtgt atgttcctgc cgatatttat gttcgctacc ttcgactgaa
     7201 aggtgaagaa gtaatattta ttggcggttc tgatgaacac ggcgtgccta tcactatcaa
     7261 agcccgcaag gaaggtgtaa gttcacagga catcgttgac agataccata ctattatcaa
     7321 agaatcattt gaagagtttg gtatttcatt cgatatatat tcacgtacaa catccaaaac
     7381 gcatcaccaa ctggcttctg actttttcag aaaactctat gatgaaggta aattcatcga
     7441 acaaaccacc gaacagtatt acgataccga ggcacagcaa ttcctggccg accgatatat
     7501 aaccggaacc tgtccgcatt gcggcaacga aaacgcttac ggtgatcagt gcgaagcctg
     7561 cggaacttca ctgaacgcaa ccgatttgat caatccgaaa tcggccatca gcggaagcat
     7621 tcccgaaatg cgcgacacaa agcactggta tctcccactc gaccagtacg aaccctggct
     7681 gcgtgaatgg atacttgaaa atcataaaga atggcggccg aatgtatatg gacagtgtaa
     7741 gagctggctc gacatgggac tgcaaccccg cgccgtgagc cgcgacctgg actggggaat
     7801 tcccgtgccg gtggaaggtg caaaaggaaa agtactttat gtatggtttg acgcgccaat
     7861 tggctacatt tccaacacca aagaactgtg cgacgcgcaa cccgaaaagt tcggcagctg
     7921 ggaaacctgg tggaaagatc cggaaacccg cctggtgcat tttatcggaa aagataatat
     7981 tgtgttccac tgcattgtat tcccttcgat gctgaaagct gacggaagtt atattttgcc
     8041 cgacaatgtt ccttcgaatg agttcctgaa cctcgaaagc gataaaatat ccacctcgcg
     8101 taactgggca gtctggctgc acgaatacct ggaagatttt ccgggaaaac aggatgtgct
     8161 acgctatgtg ctgaccgcca acgcgcccga aaccaaggac aacgacttta cctggaaaga
     8221 ttttcaggca cgcaataata atgaactcgt tgctatttac ggcaatttta tcaacagggc
     8281 attagtgctt acccagaaat atttcggagg taaagttcct ccaattggaa caaaaacaga
     8341 ttatgatatc cagaccatca gcgatttcag taaagtgaaa acagaacttg aaaatcagct
     8401 tgacaatttc catttcaggg aagctcaaaa agaagccatg aacctcgcgc gcatcggcaa
     8461 caaatacctg gccgagaccg aaccgtggaa actggcaaaa acagacatgg accgcgtagc
     8521 taccattctg aatatcagtt tacagattgc agccaatctt tctatcgcgt tcgaaccatt
     8581 tttaccattt tcctctgaaa aactccgcaa gatgctttca atgagcgaat tcgactggaa
     8641 tgaactggga agaacagacc tgcttgcaga aggattacaa cttggtactc ccgaactatt
     8701 gttcgaaaaa attgaagatg aacaagtgga tgctcaggta caaaaactcg agaacaccaa
     8761 aaaagccaac gaaatttcat cctacaagcc aaaaccaata aaagaaactg tttcatacga
     8821 tgatttcgga aaactggata ttcgtgtagg aacagtgctg gaatgcacca aagtaccaaa
     8881 agccgataag ttgcttcaat tccgcattga tgacggcatg ggtggacgaa ccatcctgtc
     8941 gggtattgcg caatcctacc ccaatcccga agaactggtt ggtactcagg tttgcttcat
     9001 cgccaacttt gagccaaaaa aactacgcgg tgtgatgagc gaaggcatga tactttctgc
     9061 cgaaaattcc gacggaaaac ttgtattggt tcaaccaaaa gacattgtaa gaaacggcgt
     9121 gaatgttgga taaacattta tcaataacat tttacataga acaagaatat ttggaataaa
     9181 aacagattgc ggaatgtctg aatggcattc cgcaatctgt ttttaaaatt ccatggtgat
     9241 ttatgcagtc tgaagatccg atattttatt ttacaatact tttgcccagt cacctcttgt
     9301 aaaatcaggt atttccacgg gacagctgcc attcaggaca gataattcgg tca
//
LOCUS       DAOPYB010000116         9310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_8733_length_9310_cov_8.705781, whole genome shotgun sequence.
ACCESSION   DAOPYB010000116 DAOPYB010000000
VERSION     DAOPYB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8733_length_9310_cov_8.705781"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..2543)
                     /locus_tag="PKH58_09140"
     CDS             complement(183..2543)
                     /locus_tag="PKH58_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HNX89235.1"
                     /translation="MNQKNILITILTFTLFFVACAEKTLQLGKASVDKVVAAMTLEEK
                     AKLLVGTGMAGFSGETTVVGKTEQLVPGAAGTTYAIPRLGIPATVLADGPAGLRITPI
                     REGDEQTYFCTAFPVATTLAATWNTELVESVGKAMGNEVLEYGADILLAPALNIHRNP
                     LCGRNFEYYSEDPVVSGKMAAAMTKGIQSNGVGVSLKHFAANNQETNRMGNDSRMSPR
                     VLREIYLKGFEIAVKESNPWTIMSSYNRINGTYAPENYELLTTILRDEWGYKGMVMTD
                     WFGGSDVSAMVRAGNDLLMPGSAKQYDAIVAAIENGSVSMEDVDRNVKNILNMILATP
                     RFKGYIYSNKPDLKANATVTRQSAAEGMVLLKNNNTLPLSASVKNVAAYGISSYDFIS
                     GGTGSGDVNEAYTISLVEGLINAGYQLNKQLKIQYEEYIAAENEKNKPDSGNPLSAFM
                     PKVRPGEFVPTSSQLAQQVKESDIALITIGRTSGEFVDRMIEGDFNLTANEKTMIEVV
                     SRAFQAAGKKTIVILNIGGVIESASWKDYPDAILIAWQAGQEGGNTVADVLSGKVNPS
                     GKLPMTFPVNFMDAASSANFPSDPAVVKSISLNFPGTQRDGKESLRNVDYTNYEEDIF
                     VGYRYFDTFQKNVSYHFGYGLSYTSFEYTNPLIEDKKDDFIVSIDITNTGSVSGKEVV
                     QLYVAAPQNAALPKPSKELKAFVKTKELKAGETQTLRMSVAKSDLASFNNNKSAWVVD
                     SGSYTILLGTSSRDIKKSLELNISDPGVEKTNNVLQLQSPITVLNP"
     gene            complement(2559..3692)
                     /locus_tag="PKH58_09145"
     CDS             complement(2559..3692)
                     /locus_tag="PKH58_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX89236.1"
                     /translation="MKKIIFLSLAIFLCTTLVPAQELANFVNSKPIISPEISGNEVIF
                     RIKAAKARDVKLYGSWMANYTDRQDLTEKENGIWEVTLPTPDPEIYTYHFIVDGVVMS
                     DASNILMQRDGTRYLSMLLIDGPRSANYKEAEKRGNLTKVWYDSPTLKMNRRMYVYTP
                     RGYEKSKQKYPVLYLLHGGGGDEDAWSNMGRACQILDNLIEQGKAKPMIVVMPNGNPG
                     QQAAKPLMLAENAFDYRDPSTANLYIHSLAKDIVPFIERNYRVISKPDSRAVSGLSMG
                     GGHTMNIANNYPGMFGYIAPMSMGIREGQDIDTQLQALKKAGYKLYWLACGDADFAYP
                     GAKLLHEALDRNGLKHTFHITGGGHTWSNWRDYLNTLAPMLFK"
     gene            complement(3700..4908)
                     /locus_tag="PKH58_09150"
     CDS             complement(3700..4908)
                     /locus_tag="PKH58_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX89237.1"
                     /translation="MKRLTILAVMLLMAVTSFSQQALWGGQAIVSPEIHENNTVTFRL
                     RAPKAVKVEVTGDFLPPQPTETPFGKYDLPGTAQLKEGEGGVWEYTTPAPLASELYSY
                     TFIVDGLRINDPANVSMIRDVASVTNVFIIPGNPGDLYSIKDIKHGSVVRRWYDSPTL
                     KEQRRITIYTPPGYEGNNQSYPVFYLLHGSGGDEEAWIALGRTAQIMDNLIAQGKVKP
                     MIVVMPNGHTQNTAAPSETNRNYLPAMGGGPRQAVASMEDSFGDIIKFVESNYRVKKD
                     KANRAIAGLSMGGMHSAAISAQYPNTFNYVGVFSAPPIATMMARNNDDVAKNTEEFVK
                     KLQIQQKNGFNLYWIACGDTDFLYQSVIDSMKKMDEIGFKYTYRESGEGHIWKNWRIY
                     LSEFAPMLFK"
     gene            complement(4975..5817)
                     /locus_tag="PKH58_09155"
     CDS             complement(4975..5817)
                     /locus_tag="PKH58_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX89238.1"
                     /translation="MALFTSAQIAWGPQSKVETKVIQSKVLNAEREYSIFLPVSYDQE
                     PNRKYPILYLLHGMMDTNKGWYERGYVKDVMDQLVASGEAVEMIIVTPNAGGNIYEGA
                     WNGYFDMPGWAYETFFFTEFLPFIESTYRVDGDKGNRAIAGLSMGGGGATVYGQKHPD
                     MFNSVYAMSALMDIPEQGAAPSQIPDDKMAILTKSVQENSAVRFVEDADADQVKHLQT
                     LNWFVDCGDDDFLLDRNIELNQAMRKKQIKHEFRIRDGAHTWEYWHSALYICLPYVSR
                     NFGN"
     gene            complement(6083..8314)
                     /locus_tag="PKH58_09160"
     CDS             complement(6083..8314)
                     /locus_tag="PKH58_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HNX89239.1"
                     /translation="MTLEEKVRMVIGCGMPMGGDAKFPGTAGRTYDIPRLGIPSVYFA
                     DGPHRLAMSVRRDFDSRFYYTTEFPSGTTVAATFNPDVAFKVGEALGTEVKDYGMDVL
                     LAPGVNLMRNVLNGRNHEYYSEDPLLTGKIAAGFINGVQSKGVGTSIKHFAVNSQETN
                     RNNNDSRLDQRTLRELYLKGFEIAVKESQPWTVMTAYNKVNGKYTSEDKGLTTDILRD
                     EWGFKGLVISDWNAGQDAVASMNAGNDMLQPGQERQFKSIYDAVKNGILDEKILDRNV
                     KRVLELVVKCHSFNNYNYPNETDLKAHATVSRQAGSEGIVLLANNGVLPIDKKINQIA
                     LYGTTSYDMVPAGMGFGSTGFGRYTVSMVEGLRKASYTVDKKLTQQYQKHLQEEQKRL
                     FPNGRPPFSFTPPPRADEFLPTAEEMAAQVKLNDLAILTIGRTSGEAKDRHANEFYLT
                     KNENALLKQVSDAYHAAGKKVVVILNICSPVETESWKSMADAIICAFQPGQEVGHCVT
                     DVLTGEVNPSGKLPVTFALKYGDAPSDNNFPFDYEFKMPSFMMGSGMNVQRDQKEVKP
                     KESERNVDFTNYEEGIYVGYRYFDTFNKEVSYPFGFGLSYTTFDYEVISSSILNENCE
                     VKVKVTNSGQVAGKEAVQLYVKAPGKDLNKPSKELKALAKTKELKPGESEVVTLIWNL
                     MDMSSFNVKTSSWVLEKGIYQWLVSASSTDVRCSVNQDIARVKTMKAHNVMAPQAEIK
                     TIR"
     gene            complement(8461..9249)
                     /locus_tag="PKH58_09165"
     CDS             complement(8461..9249)
                     /locus_tag="PKH58_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX89240.1"
                     /translation="MLESNRILDTSNPFDEFGFFNVMNHWRMDNLEATSPKFNDYKDT
                     GWGFMVPQKNLYDAFVAEEGVNGYRLNHTLKTYAQMNDLGVKLQKGKTIINEGVFMWK
                     RRFTSAEAPITFWCSFNNYRWIRYAEVLLLAAEANLMAGNQSEANACLNEVRTRARLP
                     FKTANLDAIKLEKRLELCSEYTRYQDIIRWKDAERLLKNQGSVTPLLTSKATSLSEPD
                     NVVVEYRQYNTDPNRYGFKARHNLLPIPATEMRLNPNMVQNEGW"
BASE COUNT         2611 a         2101 c         1705 g         2893 t
ORIGIN      
        1 aaaacaaaga taaaacggac ttagagagtg atgttactcc cagtccgttt ttttttcaat
       61 ctataaagcg gcggatttgg gatgcttact gagcaataaa aaagtgctgt aaaaaatttc
      121 acagcacttt ttattggatc acggaaacgg gaaaacacaa gtaaccccaa gcagtctaaa
      181 cactatgggt ttaacaccgt tattggagat tgtaactgca gtacattgtt tgttttctct
      241 acgcccgggt cagaaatatt tagttccagt gactttttaa tatctcgtga tgatgtacct
      301 aaaagtatag tatagctacc ggaatccacg acccacgcac tcttattatt attgaaagaa
      361 gctaaatctg attttgcgac actcattctc agggtttggg tttctcctgc tttcaattct
      421 tttgtcttca cgaaagcttt aagctctttt gatggcttag gcaaagcagc attctgtgga
      481 gccgccacat acaactgaac aacttcttta cctgaaacac taccggtgtt tgtgatatct
      541 atacttacaa taaaatcatc ttttttatcc tctattaaag gattagtgta ctcaaaagaa
      601 gtataagata ggccataacc aaaatgatac gacacatttt tttgaaaggt atcaaaataa
      661 cgatagccta caaaaatatc ttcctcgtaa tttgtataat caacatttcg taaagactct
      721 tttccatcac gctgagttcc ggggaagttg agactgattg attttacaac tgcaggatcc
      781 gatggaaaat tggcggacga tgcagcatcc ataaaattaa ccggaaatgt cataggtaat
      841 ttgcccgatg ggtttacctt tccgcttaga acatcagcaa ccgtatttcc accttcctgc
      901 ccggcttgcc aggcaatcaa tatggcatcg ggataatctt tccatgaagc agattcaata
      961 acaccgccaa tattcagaat aactattgtt tttttgcctg cggcttgaaa agctcgggat
     1021 acaacttcaa tcattgtctt ttcattggca gtcagattaa aatcaccttc tatcattcga
     1081 tcaacaaatt cgcccgaagt acgtcctata gtgattagtg ctatatctga ttcctttacc
     1141 tgctgagcca actgtgatga ggttggaaca aattctcccg gacgcacctt cggcatgaat
     1201 gccgaaagag gattgcccga gtcaggtttg ttcttttcat tttctgcagc aatgtattct
     1261 tcgtattgaa tctttaattg tttattcaat tgatatccgg catttattaa gccctcaact
     1321 aaagagatgg tataagcttc attgacatca ccgctacctg taccaccaga gataaaatca
     1381 taagaagaaa tgccatatgc agctacattc tttactgaag ctgacaaagg aagcgtattg
     1441 ttgtttttta gtaaaaccat cccctccgcc gccgattgac gggttacggt tgcgttcgct
     1501 ttcaggtcag gtttattgga gtaaatataa cctttgaaac ggggagttgc aagaatcata
     1561 ttcaatatat tcttcacgtt gcgatctaca tcctccattg acacgcttcc attttctatg
     1621 gctgcaacaa ttgcatcata ctgtttcgcg gaaccgggca ttaacaagtc gttaccggca
     1681 cgcaccattg cagaaacatc acttcctcca aaccagtcag tcattaccat tcctttatac
     1741 ccccattcat cacgaagaat tgttgttaat aattcatagt tttcaggagc atacgtaccg
     1801 ttaattcgat tataagaaga catgatagtc cagggattgg attcctttac cgcaatctca
     1861 aaacctttaa gatagatctc gcgcaaaacg cgtggtgaca tccggctatc attacccata
     1921 cgattggttt cctgattatt ggcagcaaag tgttttaatg aaacaccaac accatttgac
     1981 tggatacctt ttgtcattgc agccgccatc tttccggaga ctaccggatc ttccgaataa
     2041 tattcgaagt tacgcccaca aagaggatta cgatggatat taagggcagg tgcaaggaga
     2101 atatcagcac catattccag cacctcattc cccatagcct tgcctacact ctctaccaat
     2161 tcagtattcc aagtggcagc taaggttgtt gcaacaggga aagcagtaca aaaataggtt
     2221 tgctcatcgc cttcccgaat aggagtaatc cgtaaaccgg cgggcccgtc agccaataca
     2281 gtagcgggta tacccaaacg gggaattgca taagttgtac ctgcagcacc gggaacaagt
     2341 tgctccgtct tacccacgac agttgtttca cctgaaaaac ctgccattcc ggttccgact
     2401 aacaatttgg ctttctcttc caaagtcata gccgccacaa ctttatcaac agatgcttta
     2461 cccaattgaa gtgtcttttc tgcacaagca acaaaaaata atgtaaaagt taaaattgtt
     2521 attagtatgt ttttttgatt cataataact ttaaataatc atttaaacag cattggagcc
     2581 aaagtattca gataatcccg ccagtttgac caggtgtgtc caccccctgt aatatgaaaa
     2641 gtgtgtttca atccattcct gtctaaagct tcatgaagga gttttgctcc cggataagcg
     2701 aaatctgcat caccacatgc cagccaatat aatttataac ctgctttttt caaagcctgc
     2761 agctgagtat ctatatcttg tccttccctt attcccatac tcatgggagc aatgtaacca
     2821 aacatacccg gataattatt tgcaatattc attgtgtgtc cgccacccat tgatagtcct
     2881 gacactgcac gagaatcggg tttggatata acccggtaat tcctttcaat aaaaggaaca
     2941 atgtcttttg ccagactatg tatatatagg ttagcagtag aggggtcacg ataatcaaag
     3001 gcgttctctg caagcattaa aggctttgcc gcctgctgac ccggattgcc attaggcata
     3061 accactatca tcggctttgc tttaccttgt tctatcagat tatctaatat ctggcaagca
     3121 cgtcccatat tcgaccaagc atcttcatct ccacctccac cgtgaagtaa ataaagtaca
     3181 ggatacttct gtttactttt ttcataaccg cgcggagtat atacatacat ccttcggttc
     3241 atttttaacg ttggagaatc ataccatact ttcgtaagat tgcctctttt ttcagcttct
     3301 ttgtaatttg ccgatcttgg tccgtctata agcaacatgc taaggtagcg ggtaccatca
     3361 cgctgcatca atatattact ggcatcgctc atcactactc catctacaat gaagtgataa
     3421 gtatagatct caggatcagg tgtaggcaag gttacttccc agatgccatt ttctttttcg
     3481 gtcaaatcct gcctgtcggt ataatttgcc atccaggagc catataactt tacatccctg
     3541 gcttttgccg cctttatacg aaagattact tcgttgcctg atatttcagg tgaaattatc
     3601 ggtttactat tcacaaaatt agcaagctct tgtgctggta ctaaagttgt gcataagaaa
     3661 atggcaagtg ataaaaaaat aattttcttc atgactttgt tatttaaata acattggtgc
     3721 aaactctgat aaataaatcc gccagttttt ccaaatatgc ccttcaccgc tctcgcgata
     3781 agtgtatttg aatccaattt catccatctt tttcatactg tcgataacac tttggtacaa
     3841 aaagtctgta tcaccgcagg caatccagta tagattgaac ccattctttt gctgaatttg
     3901 tagttttttt acaaactcct cagtattttt agctacgtcg tcgttgttgc gagccatcat
     3961 tgttgcaata ggaggagcag agaatacacc tacgtaatta aaagtattcg gatattgagc
     4021 tgagatagca gcagaatgca ttccacccat agataaaccg gcgatagcac ggttagcttt
     4081 gtccttctta acacgataat tactttcaac gaattttatt atgtcaccaa aactatcttc
     4141 catactggca acggcctgac gtgggccacc gcccatagcg ggtaaataat tacgatttgt
     4201 ttcacttggt gcagctgtat tctgtgtatg tccgtttggc ataacaacaa tcatgggttt
     4261 aacctttccc tgagcaatca aattatccat tatttgggca gttcgtccta gagcaatcca
     4321 tgcttcttca tcgccacccg aaccgtgaag gagataaaac acggggtaac tttgattatt
     4381 gccttcatat ccgggaggag tataaatagt tatacgacgt tgttctttta atgtcggact
     4441 gtcataccat ctccgtacaa cactaccgtg cttaatatct ttgatgctat agagatcacc
     4501 cggatttcca ggtatgataa acacattcgt taccgatgct acgtcacgaa tcatggacac
     4561 atttgcagga tcattgatgc gcaaaccatc aacaatgaat gtataactgt aaagttcaga
     4621 ggccaacgga gcaggagttg tatattccca aactccacct tcaccctctt tcaattgagc
     4681 cgtaccgggc aaatcatatt ttccaaaagg ggtttcagtt ggttgaggtg gcaagaagtc
     4741 accggttact tcaaccttca cagccttcgg cgctctcaac cggaaggtca cggtgttgtt
     4801 ttcatgaatc tcaggagaaa caatagcttg tcctccccaa agagcttgct gtgaaaatga
     4861 tgtcacagcc ataagcagca tgactgccag aatagttaat cgtttcatat tcaaatgttt
     4921 taaaaattta tatattggta tttcgttgtg caatgttgca atagcttcaa ttaattagtt
     4981 tccgaagttc cgtgaaacat atggtaagca tatatacaac gctgagtgcc agtattccca
     5041 tgtgtgtgct ccgtcgcgta tccggaattc gtgcttaatt tgttttttcc gcattgcctg
     5101 attgagttcg atatttcggt cgagcaagaa gtcatcgtca ccacagtcca caaaccaatt
     5161 caacgtttgc aagtgtttta cctggtctgc gtcagcgtct tctacgaagc ggacagcgct
     5221 attctcctgt accgattttg taaggatagc catcttatca tcaggaattt gagatggagc
     5281 agcaccttgt tcaggtatat ccattagggc gctcatagcg tataccgagt tgaacatatc
     5341 agggtgcttt tgcccataaa ccgtagctcc acccccgccc attgacaatc ctgcaatagc
     5401 cctgtttccc ttgtctccgt ccacccggta agtggattct atgaaaggga ggaattcagt
     5461 aaagaagaag gtttcgtatg cccagccagg catatcgaaa tagccattcc aggcgccttc
     5521 ataaatattt cctcctgcat ttggagttac aataatcatt tctactgcct cacccgaagc
     5581 aaccagctga tccataacat cttttacata ccctcgctca taccaaccct tgttggtatc
     5641 catcataccg tgaaggagat aaagaatagg atattttctg tttggctcct gatcataaga
     5701 aacaggtaaa aaaatgctat actcccgctc agcattcaat actttacttt gaatcacttt
     5761 agtctcaact ttactctgcg gcccccaagc gatttgtgcc gaagtaaaaa gagccatgct
     5821 aaatagcaat gttaaaaaac agttcgtttc atgtgtttat attttatttt ttacattatg
     5881 atcacatgcc tgcctgtcga cagacaggga atctttgacg atttcatatt attattttta
     5941 gattttctat atatcgaatg aagtcaatca attcaaaaca gattctaaat aaaatatggt
     6001 caatttgatg aatttacgta ttcaaagata atttattctt gtttcagatt aagttgagag
     6061 gcttttattg cttctctctt aattatcgaa ttgttttgat ttctgcttga ggagccatga
     6121 cattgtgtgc cttcatcgtc tttactcgcg caatatcctg attaacagag cacctcacat
     6181 cagttgatga ggcagacacc agccattggt atattccttt ttccaatacc catgaacttg
     6241 tttttacatt aaacgatgac atatccatga ggttccatat taacgtaact acttcgcttt
     6301 caccgggctt caactccttc gttttagcca aagccttaag ttcttttgat ggtttattca
     6361 agtcttttcc gggtgctttc acatagagct gaacagcttc tttacctgct acttgtccac
     6421 tattagtaac cttcactttt acttcgcagt tctcgttcag gatagacgaa ctaattactt
     6481 catagtcaaa tgtagtataa gagagtccaa aaccaaacgg atatgaaact tctttgttaa
     6541 acgtatcaaa ataacggtac ccaacataaa taccttcctc ataattagtg aaatccacat
     6601 tgcgttccga ttctttcggt ttaacttcct tctgatcacg ttgcacattc atgccgctac
     6661 ccatcataaa tgaaggcatt ttgaactcgt aatcaaaggg aaaattgtta tcagaagggg
     6721 cgtctccata cttcaaggca aacgtaaccg gaagtttacc cgatggattg acctctcctg
     6781 tcaacacatc agtaacacaa tgaccaacct cctgccccgg ttgaaaggca cagatgattg
     6841 cgtccgccat tgatttccat gattcagttt caacaggact gcagatgttg agaattacaa
     6901 ccactttctt tccggcagca tgataggcat ccgatacctg ttttagtagg gcgttttcat
     6961 ttttggtaag ataaaattcg ttggcatgtc ggtcttttgc ttcaccgctg gtacgcccga
     7021 ttgtaagaat cgccagatcg tttagcttga cttgtgccgc catttcttca gcagtgggaa
     7081 gaaattcatc tgcccggggt ggcggagtaa aagaaaaagg aggacgtccg ttaggaaata
     7141 atcttttttg ctcttcttgt agatgttttt gatattgctg agttaatttt ttatctaccg
     7201 tgtaggaagc cttacgaaga ccttcaacca tggaaacagt atatcgccca aaaccggtac
     7261 ttccgaaacc cattcctgcc ggcaccatat cgtaagatgt tgtaccatag agtgcaatct
     7321 gattgatttt tttatctatt ggaagaacac cattgttggc cagcaaaacg atcccctctg
     7381 agccagcctg tcttgaaaca gtagcatgtg ctttaaggtc tgtttcgttg ggatagttat
     7441 agttattaaa gctatgacat ttaactacca gctcaagcac gcgctttacg ttcctgtcta
     7501 aaattttctc gtctaaaatc ccattcttaa cagcatcata tattgactta aactgacgtt
     7561 cttgccccgg ctgtaacata tcatttcctg cattcataga ggcaactgca tcttgtccgg
     7621 cattccagtc tgatattacc agacctttga acccccattc atctcttaag atatcagttg
     7681 ttaatccttt atcctcgctc gtatactttc cattgacctt attataagct gtcatgactg
     7741 tccacggctg ggattcttta accgcaattt caaaaccttt aagatacagt tctctcaatg
     7801 ttcgttggtc aagtcgggaa tcgttgttat tgcggtttgt ctcctgacta tttacagcaa
     7861 agtgcttaat actggtgcct acacctttac tttgaacccc attaatgaag ccggctgcaa
     7921 ttttaccggt caataaagga tcttcggaat aatactcgtg attgcgtccg ttgagaacat
     7981 ttctcatcag attcacaccg ggagctaata gcacatccat cccataatct tttacttcgg
     8041 taccaagagc ttcgcctacc ttaaatgcta catcgggatt aaaggtagcg gctactgttg
     8101 tgcctgaagg aaattcagtg gtataataga accggctatc gaaatcgcgt cgaacgctca
     8161 tagccaaacg atgaggtcca tcggcaaaat aaaccgatgg aatgccaagc ctgggaatgt
     8221 catatgttcg tcctgcggtt ccggggaatt tagcatcacc acccataggc atgccacaac
     8281 caattaccat tcggactttt tcctccaacg tcattacttt aacaatctca tcgatattgt
     8341 ttttattaag cgttatctgt tgtgcaataa tatttgcctg gctaaatagt atagccagaa
     8401 aaataataaa acaagttttt ttcattttat aagaataatt tttcatttta attttgctca
     8461 ttaccaacct tcattctgaa ccatattagg attcaaacgc atctcagttg cagggatggg
     8521 cagaaggttg tgtctggctt tgaagccgta acgattcgga tctgtattgt actgtctgta
     8581 ttcaactacc acattgtcag gttcacttaa agaggtcgcc ttgcttgtta gtaaaggcgt
     8641 tacagaaccc tgatttttca ataaacgctc agcatccttc caacgtataa tatcctgata
     8701 tcttgtatac tcactgcaca gttctaatcg tttctctaac ttaatagcat ccagattggc
     8761 agtcttaaac ggtaatctgg cacgagtacg aacctcgttc agacaggcat tagcctctga
     8821 ttgatttcct gccattaaat ttgcctcggc agcaagcaac aggacttccg catagcgtat
     8881 ccagcggtag ttattgaaag aacaccagaa tgtaataggc gcttctgcag atgtaaagcg
     8941 acgcttccac atgaaaacgc cttcatttat aatggttttc cctttttgta attttacacc
     9001 taagtcgttc atctgagcat aagttttcaa tgtgtgattc agacgatatc cattaactcc
     9061 ctcttctgcg acaaatgcat catataaatt cttttgaggc accataaacc cccagcccgt
     9121 gtctttgtaa tcattaaatt ttggagaagt tgcttccaaa ttatccatac gccagtgatt
     9181 catcacattg aagaagccaa actcatcgaa aggattactt gtgtcaagaa tacgattact
     9241 ctccaacata ctttctcctt ttcgtttctt actccccctt tagggggttg gggggcttgt
     9301 cttctttttt
//
LOCUS       DAOPYB010000117         9249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_8817_length_9249_cov_9.761692, whole genome shotgun sequence.
ACCESSION   DAOPYB010000117 DAOPYB010000000
VERSION     DAOPYB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8817_length_9249_cov_9.761692"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            459..1067
                     /locus_tag="PKH58_09170"
     CDS             459..1067
                     /locus_tag="PKH58_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNX89241.1"
                     /translation="MRKKIFTLMIAITCAASGLLAQNEYIGEIRIFAINFAPRGWMPC
                     EGQLLPISQNTALFALLGTTYGGNGRTNFALPNLQGRVALNPGQGPGLTTRYLGEQAG
                     TATVTLLQNQMPIHNHPLFFTDAQAITNVPASDVMPAFNASLNFPGITKPVNTYAVPG
                     VIVPFAPQTMGVTGGNMPHNNMQPYLVLNYFIAVQGIFPPRP"
     gene            1163..1765
                     /locus_tag="PKH58_09175"
     CDS             1163..1765
                     /locus_tag="PKH58_09175"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNX89242.1"
                     /translation="MKRKLISFFIALFIIANIQAQDQYLGEIKICSFDFAPMGWAFCE
                     GQLLPIAQNQALFSLLGTTYGGNGTTNFALPDLRGRMVIGTGTGYLLGQRGGEEAHTL
                     VINEMPMHNHVMNGTSDPATTDKPTSTPMAQMMLAAPTDIVFPQVTKKVNTYTAPGSY
                     VQLSSQTSGSAGGSQPHNNMMPYITVRYIIAIQGIYPSRN"
     gene            1893..2495
                     /locus_tag="PKH58_09180"
     CDS             1893..2495
                     /locus_tag="PKH58_09180"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNX89243.1"
                     /translation="MKIKLSILIIALAFATQIKSQEQYIGEIRLFAGNFAPLGWAFCQ
                     GQILPIAEYDALFSLIGTTYGGDGQTTFALPDFRGRVPVAVNPNSHAGVIGTLGGTEN
                     VTLTIGQIPLHNHPILATDATGTTNVPSSDLMPAKAADMQFPGGIKQIMTYAKDNAGG
                     KIQANPQSVNPQGLSQPHDNMKPYVGINYIICLEGIYPTR"
     gene            2520..8360
                     /locus_tag="PKH58_09185"
     CDS             2520..8360
                     /locus_tag="PKH58_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNX89244.1"
                     /translation="MKINIKSLNARYTNLQMFRQKYGLTMLLLMLSFQLNAQTDTLKI
                     LNPVSPENAGGIVTAAEPFPDPMGLPTKYYTTYIPGRIIQLANAADEDSALKGIPAGS
                     LAVYNSSFAGSLYPGIVSLNGTTGIAESDNNRPKYLRVLDGPGFEFSFRSVYICDVVG
                     AQSSIKVEGFRDKVSTGSVILTRNTATEWQKTFGPSSFPLNIFGNVDEVRFSRGTADG
                     FTGNLVMFNNFVIDPPILPPYVSVSQTALNMDVGGSAVTFNITSQIDWTITGNQSWLT
                     PDVTSGSGNKTISLTGTFNNSTNQRKDTITISGAGESYSVIITQDGIPNCDDGDPHTL
                     DRFDVATMNCVHVPDTDGDGVADTNDNCSQVYNPDQKDTDGDGIGDVCDDPIVSLSQS
                     NTSFAEALGQNTITATLNRTFSENVIVNIGANVAGSAKPAVDFTMQPTIIIPAGQLTG
                     TATLTAIQDELVENDETITIDVLSVVNAVPNGNLHLTSTINDDDVATIRVARSSDGYE
                     GVRNCSFRIFTDKRIDRNVTVNLSASGTAAVGTDYTTIPVSFTLTANTDSLIIPVSVL
                     DDAEIEADESIVLLLQSTDYSKAMLAASPANAATVTIFDKPVVTLSQSVSSFAENGGQ
                     NTITVSLNKPWIADVTVKFGSLASGTATPVADFTFPDSIKIPAGSLTGMVILTAVNDQ
                     LVESDEMVVVEISSVQNGMESGDQYLFSVIMDDDIAYLRADKGADCYEGGKNGFFRII
                     TDKRFDKNVTVDISVEGATAAAGSDYIALPHSLIFPANTDTLSVPLIGIDDSESEIDE
                     TVTIQIRNTNVPRVFVSHYPVCAATATIIDDELPLVTLGQSISTFAEASGQNVLTATL
                     NKTYTSDVIVTLGANATGTATPATDFILPATITIPAGNLTGTATITTVSDQLVESDET
                     VIVDIVSVLNAVENGNQQVITTLLDDDTAMLRVTRGNNCYENGPDGSFLIITDKRFDK
                     DVKVDISVQGTATSVSDYSIIPFTQVFPSNTDTLTIPVRGMDDSESEINEYVILNLLR
                     TDYAKVGIAPAPDSIATITLFDDDMPLVTLTQSVSTFAEASGQNVLTATLNKVHYDDV
                     VVTLGANISGTASPVTDFMLPATITIPAGSITGTATISAVPDQVVERDETIVVDILSV
                     VNGIENGTQQLTSTLVDDDVAVISVVSATPIAEGFNLGLFTLKTDKQFDFDVRVDFTL
                     SGDATEGVDYQPAPMGRTIIFPKNTDSTVFLVPLLDDMLIEEDEQIVLALTATSESDV
                     HIAAGPDAVAAITIIDNEQIQLQVTAPVLTTSKVYNGLVTADVTTGTLINVRKNDQVT
                     VSAQAFYDNAKAGTGKTITIRYTLGGPAAGRYIAPVDVVYNAGIIEKALLTVTADDKT
                     KVMGHENPELTCRYSGFKANENVTNLTTAPVATTTAATDSPWGNYPISVSGGEADNYS
                     FAYIGGTLTVLDNSIIYVKNGSHGTGISWTDAVGELSDALLIAEQNPKIKQIWVAAGT
                     YKPAVEVGGTGERYKSFSLKNGVAVYGGFAGAEPRNSDISLRDFANNQTVLSGDIGIE
                     NDNSDNCYHVFNHPDGTALNQSAILDGFTVTGGNADGYPDNNFGGGICNYSSSPLISN
                     VAFVGNAATFGGGIFNKNSSPVMTNVVFDSNTAGMAGGAMYNSISSPVIIKAVFKSNV
                     AGNWGGAMANIHRSSPVLANVLFVKNTAKVYSGGAIYNNEYSSPVLTNVTLTGNTASN
                     LSNGAGGAMANITACSPVLNNCIIYGNKAGKMGNELYITGGNTSVNYSAYSNGKNDVV
                     IPAGTFTPASSIVLTPESVLFTDIYNGNFTLASGSNIINRGNNALYSVALSGMNDLAG
                     NERIVGSSIDMGAFENQTDLGTGISTGSIVKLQLYPNPVTDVLYLSGVDNNAFISITD
                     MTGRVIINKNMKGKSLPVSHLTEGVYTIKIIDANGTKTAKFVKKGR"
     gene            complement(8528..8719)
                     /locus_tag="PKH58_09190"
     CDS             complement(8528..8719)
                     /locus_tag="PKH58_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNX89245.1"
                     /translation="MNNGTVKFFNEAKGFGFIKDTNSSTEYFVHISGLVDRINENDEV
                     TFDIAEGRKGLNAVNVKLA"
     gene            complement(8958..>9249)
                     /locus_tag="PKH58_09195"
     CDS             complement(8958..>9249)
                     /locus_tag="PKH58_09195"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNX89246.1"
                     /translation="GLSCFSPFNLPGNIDSALRETFNEISEIVEGLPWYQGDHSIFLQ
                     NGCPAIAVSSQWFIENMEQQELTHTPKDNLNIVNYQRVAECAIGIAEFIGKL"
BASE COUNT         2751 a         1868 c         1948 g         2682 t
ORIGIN      
        1 tgagaaatta agtaaaaatt aaaaaaaata tggatattaa atgtttttaa ttgaaatatt
       61 aatatatttg taacacaaat tccaacataa ccagtttact tcatttttga aggcggttat
      121 tccttataca ttgatcgatt taaatctgta aagctgttgt caatacttgt gtgtatcctg
      181 aataattgat aacttcatta ttccctgaaa agatgataca taagtggtgg atgtgttatt
      241 aaatccgttt attcggtttt gtgataatgc taaatttggt tttccttttt tatttataaa
      301 tggcacattc gcatccgttt taatttacat gctgattttt cacaagactt aaatttttat
      361 ttcagttttg agaattcgaa ataaacatac cgttactttt ttgtaatgtt aattatgtct
      421 gaaaattact atgttaatca aataataatc aattaagtat gagaaaaaaa attttcactt
      481 taatgattgc catcacctgc gcggcatcag gtctcctggc tcagaatgaa tatatcggag
      541 aaatacgaat ttttgcgata aattttgccc caagaggctg gatgccatgt gaaggacaac
      601 ttttacctat ctcccagaat acagctttgt ttgctctttt gggaactact tacggaggta
      661 atggccgaac caactttgct ttgcctaacc ttcagggtcg ggtggcctta aatcccggtc
      721 agggtcccgg acttactact cgttatttag gagaacaagc cggcaccgct acagtaacat
      781 tactacagaa tcagatgcca atacacaatc atccgctttt ttttaccgat gcgcaagcta
      841 tcaccaatgt accggcatcg gatgtaatgc ccgctttcaa tgcttcactt aattttccgg
      901 gaataacgaa gccggtaaac acttatgcgg ttcccggtgt gattgttcct tttgctcccc
      961 agactatggg cgttaccgga ggtaatatgc cccataacaa tatgcaaccc tacctcgtac
     1021 tcaattattt cattgctgtt cagggtattt ttcctccaag accataatcc aacactgata
     1081 tggaattatt aataattatt attggatgat atacatcttc ccttataaat ataagttcat
     1141 tataaccgaa aaatataaac aaatgaaacg aaaattgatt agttttttta tagctctttt
     1201 tatcattgca aatatacagg cacaagatca gtatctgggt gaaataaaaa tatgttcatt
     1261 tgatttcgca ccgatgggct gggctttttg tgaagggcag ttgcttccga ttgctcagaa
     1321 tcaggcatta ttcagcttgc tgggaacaac ttacggtggt aacggcacaa ctaattttgc
     1381 actacctgat ctaagaggtc gaatggtaat aggaactgga accggatatc tacttggcca
     1441 aagaggtggt gaggaagctc atacacttgt tataaacgag atgcctatgc ataatcatgt
     1501 tatgaatgga accagtgatc ctgctactac cgataaaccg acaagtacac cgatggcaca
     1561 aatgatgctt gccgcaccga cagacattgt cttcccacag gtgactaaaa aagtaaatac
     1621 ctacactgct cccggttcat atgtgcaatt atcatctcaa acctctggtt cagcgggtgg
     1681 atcgcaacca cataataaca tgatgcctta tattacagtg agatacataa tagcgattca
     1741 ggggatatat ccttcccgca attaatcctt atcctgtttt ataaatataa aaaacaataa
     1801 taataaagta tgaaacgagt gtctatcagt tgttaataac tgattaaata ttatatgata
     1861 gtctcataca acaacaataa aaataactat ctatgaaaat aaaactatca attctgataa
     1921 ttgcattagc atttgccaca caaattaaat cacaagaaca atacataggt gaaataagac
     1981 tatttgctgg aaattttgca ccgctgggtt gggcattctg tcaaggccag atattgccaa
     2041 tagccgaata tgatgctttg ttcagcctca tcggtacaac ttacggtggc gatggacaaa
     2101 ccacttttgc cttacccgat ttcagaggga gagtacctgt agctgtaaat ccaaactccc
     2161 atgctggggt tataggtaca ttgggtggaa cggaaaacgt aactttaacg attggacaga
     2221 tcccattaca taatcatcca atattggcaa ctgatgcaac cggaacaaca aatgttccgt
     2281 catctgatct catgcccgct aaagctgctg atatgcaatt tccgggagga ataaaacaga
     2341 ttatgactta tgctaaggac aatgccgggg gaaaaatcca agctaatcct cagtcggtta
     2401 atccgcaggg attatcgcag ccgcatgata acatgaaacc ctatgttggg attaactata
     2461 taatatgtct ggagggaatt tatccgacac gatagtaacc ttataataaa gaaaaggata
     2521 tgaagattaa tataaaatca cttaatgcca ggtacactaa ccttcagatg tttagacaga
     2581 aatacggctt gacaatgttg ctgttaatgt tatcgtttca gcttaatgct cagaccgata
     2641 cattgaaaat attaaatccc gtttctcctg aaaatgcagg aggtatagtt acagcagcgg
     2701 agccctttcc tgatcctatg gggctaccca caaagtatta tactacctat ataccgggca
     2761 gaatcattca gttagccaat gcagcggatg aagattctgc attgaaaggt attccggcgg
     2821 gaagtttagc tgtttacaat tcttcttttg caggctcgtt gtatccgggc attgtttctc
     2881 tgaatggtac aaccggaatt gccgaatcag ataataaccg tccgaaatat ctccgtgttt
     2941 tagatggccc tggttttgag ttcagcttta gatcggttta tatctgtgat gtggtgggag
     3001 ctcagtcttc tattaaggtg gaaggttttc gcgataaagt atcaacagga tcagtcatac
     3061 tgacccggaa tacagccaca gaatggcaga aaacattcgg accatcgtca tttccactta
     3121 atatttttgg caacgtggac gaagtgcgtt tttcacgcgg aactgccgat ggctttaccg
     3181 gcaatctggt aatgttcaac aattttgtca ttgatccacc cattcttccg ccatatgtta
     3241 gtgttagcca gaccgctctg aacatggatg ttggaggaag tgcagtaacc tttaatatca
     3301 cctcacagat tgactggacc ataaccggca accaatcctg gctgacaccc gatgtaacaa
     3361 gcggcagcgg aaacaaaacc atctcgctga ccggaacatt taataattca accaatcagc
     3421 gtaaagatac aattacaatt tcgggtgcgg gtgaaagcta ttctgtaatc attacacaag
     3481 acgggatccc taattgtgat gatggagatc cgcatacatt ggaccggttc gatgtggcta
     3541 caatgaattg tgttcatgtg cccgacaccg atggtgacgg agtggctgat acaaatgata
     3601 attgcagtca ggtgtacaat ccggatcaga aagataccga tggagatggt attggtgatg
     3661 tctgtgacga tccgatagtt tctctttctc agtcaaatac atcttttgcg gaagctctcg
     3721 gtcagaatac tataacggct acactcaatc gcacatttag tgaaaatgta attgtaaata
     3781 ttggcgcgaa tgttgcgggg tccgcaaaac ctgccgttga ttttacaatg cagcccacta
     3841 taatcatacc tgcaggccaa ttgacaggaa cagcaacact tactgccatt caggatgaac
     3901 tggttgaaaa tgatgaaact atcacaattg atgtattatc ggttgtcaat gcagtaccta
     3961 acggaaatct tcatctgacc tccaccatca atgatgacga tgtggctaca atacgggtcg
     4021 caagatcatc cgacggttat gaaggggtaa ggaattgctc gttccggatt tttaccgaca
     4081 aacggattga cagaaacgta accgtgaatt tgtcggcaag cggaacagcc gctgtaggaa
     4141 ctgattatac cacaataccg gtttcattta cacttacggc aaataccgac tcgctgatta
     4201 tacctgtatc ggtactggac gatgctgaaa ttgaagccga tgaaagtata gttttattgc
     4261 tgcagagtac tgactattca aaagctatgc ttgccgcgtc gcctgctaac gcggctacag
     4321 ttaccatctt tgataaaccg gttgtaaccc tttcacaatc tgtcagtagt ttcgctgaga
     4381 atggtggaca aaacacgatt acagtttcgt tgaacaagcc ctggattgca gatgtaactg
     4441 ttaaatttgg ttcgttagca tctggaacag ccacaccggt tgctgatttc actttcccgg
     4501 attctataaa aataccggca ggcagtctga ccggaatggt tattctgact gctgtaaatg
     4561 atcaactggt agaaagtgat gaaatggttg ttgtagaaat atcctcggtt cagaatggaa
     4621 tggaatccgg agaccagtat ttgttttctg tgatcatgga tgatgatatt gcatatctac
     4681 gtgccgataa gggagccgac tgctatgaag gtggtaagaa cggctttttc aggattataa
     4741 ccgacaaacg tttcgacaag aatgttacag ttgacatctc tgttgaggga gcaacagctg
     4801 ctgccggatc tgattatatc gcgcttccac attcactaat ctttcctgcc aataccgata
     4861 cactgtctgt gccgttgata ggtattgatg attcggaaag tgaaattgac gaaacagtca
     4921 caatacaaat aagaaataca aatgttccca gagtatttgt ttcacattat cctgtttgtg
     4981 ctgccacggc tacaatcatt gatgatgaac tgccactggt aacacttggg caatcgataa
     5041 gcactttcgc ggaagcatcc ggtcagaatg tacttactgc cactctgaat aaaacttata
     5101 caagcgatgt gattgtaaca cttggagcaa atgcaacagg aactgctaca ccggcaactg
     5161 acttcatact gccggctacc atcactattc ctgccggaaa cctgacaggt acagctacca
     5221 tcacaactgt ttctgatcaa ttggttgaaa gtgatgaaac tgtaattgtt gatatcgttt
     5281 cggtcctgaa tgctgtggag aacggcaatc aacaggttat tacaaccctg ttggatgacg
     5341 ataccgcaat gctgagagta acaagaggaa acaattgcta cgagaatggt cccgatggtt
     5401 cattcctgat tattacagac aaacgtttcg acaaggatgt aaaagtggat atttctgtac
     5461 agggaacagc tacttccgta tctgattatt ccatcattcc ttttacccag gtattcccgt
     5521 caaatacaga tacattgaca atacccgttc gtggaatgga tgactcggaa agtgaaataa
     5581 acgaatatgt tatattgaat ttgcttagaa ctgattatgc gaaagtaggt attgccccgg
     5641 ctcctgattc tatagcgacc attactctct ttgacgatga tatgccgctg gttactctca
     5701 ctcaatcagt cagcactttc gcggaagcat cgggtcagaa tgtgctaaca gcgacactta
     5761 ataaagtcca ttacgacgat gtggttgtaa cacttggtgc caatatttcg ggaactgcct
     5821 cgcctgttac tgactttatg ctcccggcta ccatcacaat tccagccgga agtatcaccg
     5881 gaacagctac aatctcagct gttcccgatc aagttgttga aagggatgaa acaatagttg
     5941 ttgatatcct ttcagttgtc aacgggatag aaaatggtac gcagcagctc acttcaaccc
     6001 tggttgacga cgatgttgct gtgatttcag tggtgtcagc cactccgata gctgaaggtt
     6061 ttaaccttgg attgtttaca ctgaaaacag acaaacagtt cgatttcgac gtgcgggttg
     6121 attttaccct ttcgggcgat gctaccgaag gtgtcgatta tcagccagcg cctatgggaa
     6181 gaacgataat atttccaaag aatacggatt caacagtctt tcttgtacct cttcttgacg
     6241 atatgcttat cgaagaggac gaacagattg tactagccct gacagctact tccgaatccg
     6301 atgtgcatat agcagccgga ccagatgccg ttgctgccat tactattatt gacaacgaac
     6361 agattcagct gcaagtcaca gcgccggtgc ttacaacatc aaaagtgtac aacggacttg
     6421 tgacggcaga tgttacaacc ggtacgttaa tcaatgtgcg gaaaaatgat caggttactg
     6481 tttcagctca ggcattctac gataacgcga aagctggaac aggcaagaca ataacaattc
     6541 gttacaccct tggaggacct gccgccggaa gatacattgc acctgttgac gtggtttaca
     6601 atgccggtat aatagaaaag gcattattaa ccgtaactgc cgatgacaag acaaaagtaa
     6661 tgggacatga aaatccggaa ttaacctgtc ggtatagcgg ttttaaagct aatgaaaatg
     6721 taactaatct tacaacagct ccggttgcca caacgactgc tgctacagac agtccgtggg
     6781 gcaattatcc gattagtgtt tcgggcggag aggctgataa ctacagtttc gcatacattg
     6841 gcggaacgct cacggtgctg gacaatagca ttatctatgt taaaaatggt agtcatggta
     6901 caggaatatc ctggaccgac gcggttggtg agttgtcgga tgcgctgctg atagcagaac
     6961 aaaatccgaa aataaagcag atatgggttg ctgccggaac atataaacca gctgttgaag
     7021 ttggtggaac cggtgaaaga tacaaatcct tcagcttaaa gaacggggtt gcggtgtacg
     7081 gtggcttcgc gggagcggaa cctagaaact cggatatatc tcttcgtgat ttcgcgaata
     7141 atcaaactgt cttaagtggc gatatcggca ttgaaaatga caactcggac aattgttatc
     7201 atgttttcaa tcatcccgat ggtacagcac ttaatcaatc agctatactg gatggtttca
     7261 cagtaaccgg gggtaacgcc gatgggtatc ctgacaacaa cttcgggggc ggtatttgca
     7321 attattcttc ttcaccgctt atcagcaatg ttgcttttgt tggcaatgcc gctacttttg
     7381 gagggggtat attcaacaaa aactcttcgc cggtaatgac aaatgttgtc tttgattcaa
     7441 acacagccgg aatggcaggt ggtgcaatgt ataacagcat ttcctcgccg gtgatcatta
     7501 aagctgtatt caaatcgaat gtggccggta actggggtgg agcaatggct aatatccatc
     7561 gttcatctcc tgttcttgct aatgtattgt ttgtgaaaaa taccgctaag gtatattcag
     7621 gtggagctat atacaacaac gagtactcct cacctgtact gacaaatgta accctgacgg
     7681 gtaatactgc atcgaacctg tcgaatggtg cagggggtgc tatggctaat attaccgctt
     7741 gttcacctgt tctgaataac tgtatcatct acggtaataa ggcggggaaa atgggtaatg
     7801 agctttatat taccggaggt aatacttccg tcaattattc ggcttactcc aacggcaaaa
     7861 atgatgttgt aattcctgcg ggaactttca ccccggcatc cagcatcgta cttacccccg
     7921 aaagtgttct gtttacagat atttataatg gcaattttac tctggcttcg ggtagcaata
     7981 ttatcaacag aggcaacaac gcgctgtatt ctgtagcatt atcgggtatg aatgatctgg
     8041 ccggcaatga acgtattgtt ggtagcagca tcgatatggg cgcttttgag aaccagactg
     8101 atttaggtac aggtatatct actggcagta ttgttaaact tcaactgtat cccaatcctg
     8161 taaccgatgt actctatctg tcgggtgtgg acaataacgc atttatcagc ataactgata
     8221 tgactggtcg ggtaatcatt aataaaaaca tgaaagggaa aagcttacct gttagccacc
     8281 tgaccgaagg tgtttatacg attaagatta ttgatgccaa tggaacaaaa actgcgaagt
     8341 ttgtgaaaaa aggaaggtaa tcagtctatt cctttagatt tcactgtttt ttattttagt
     8401 agtagtccgc ctggttgcac gatgcagtca ggcggatttc attacatgat gttttaaaac
     8461 aaaaaagtct tacaaattag taagacttta aagtgatgac tgctatataa tgataattat
     8521 ccgcttatta ggctaatttt acatttactg catttaatcc ttttcttcct tcggcaatgt
     8581 cgaatgttac ctcatcgttt tcgttgatac gatcaaccaa acccgaaatg tgtacaaaat
     8641 actcggtgga ggaattggtg tctttaataa agccaaagcc tttcgcctca ttgaaaaatt
     8701 ttactgttcc gttattcata aaaattgttt ttattattat gccgtaaaga taaggatatt
     8761 atttggataa taattttttc actaattttt ttacaggtaa acttctgatt ttctatagtg
     8821 aaagtgaaca gtattaataa tatttacccg ttccggaatt caccgggcga cattcccgtg
     8881 tggaatttga aataattcga cagataagca gggtagtagc aaccatggta agcagaatga
     8941 caggcgttca gtttttttta caatttccct ataaactccg caattccgat tgcacattct
     9001 gccacccgtt gataattgac aatgttcaga ttatcttttg gtgtatgagt aagttcctgc
     9061 tgttccatgt tttcaataaa ccattgtgaa ctaaccgcga ttgccggaca tccgttttga
     9121 agaaatatgc tgtgatcgcc ctgataccag ggtaagcctt caacaatttc agaaatttca
     9181 ttaaacgttt cacgcagcgc gctgtcaatg ttgccgggaa gattgaaagg agaaaaacag
     9241 gataaaccc
//
LOCUS       DAOPYB010000118         9231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_8842_length_9231_cov_8.340344, whole genome shotgun sequence.
ACCESSION   DAOPYB010000118 DAOPYB010000000
VERSION     DAOPYB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8842_length_9231_cov_8.340344"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PKH58_09200"
     CDS             complement(<1..757)
                     /locus_tag="PKH58_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89247.1"
                     /translation="MKQFIKISVILLSVAAAFSACEKKPEVENNKINFTRAKANYLGN
                     IYRNNVNAIEAYFFDENLGTDAEGYITGSGTFLFLDMNTNFNATSIPAGTYNVNNENS
                     NENIEYSFQRGKMGLNNNNEEVPTGSYFAIVKNGSITDYLFLSSGSIIVNKMGNYSIS
                     GQVTTSDNEFYEFSYSGNIPTTDLVTPYPETLTKGQLWYWGDIDNVGLNIYTIWLADQ
                     NINMTNMSGSGDLMRIEVYTPTSYTTSLPNGTYP"
     gene            complement(915..1172)
                     /locus_tag="PKH58_09205"
     CDS             complement(915..1172)
                     /locus_tag="PKH58_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNX89248.1"
                     /translation="MKSGIHPENYRPVAFRDMSNGETFISRSTVNTKETVDIDGVTYP
                     LVKLEISSSSHPFYTGKSKLVDTAGRVDKFMSRYGNRGEKK"
     gene            complement(1346..2128)
                     /gene="truA"
                     /locus_tag="PKH58_09210"
     CDS             complement(1346..2128)
                     /gene="truA"
                     /locus_tag="PKH58_09210"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNX89249.1"
                     /translation="MQAKHYLCSVKRRYFIWFDYNGSGYCGWQIQPNGLSVQHVLTNA
                     LCTILRTEIELVAAGRTDAGVHARPMVAHFDFEGDFPVEMDLVSKLNSFLPSDISVRN
                     IREVKPDVHARFDAISRRYEYHILLHKDVFRHEFAMRIYHPLDFDRMNVAAKTLFDYT
                     DFTSFSKLHTDTKTNNCKIKHAHWEQRGEEWIFTIEADRFLRNMVRAIVGTLLEVGRG
                     KMTIEDFRKIIEAKNRCDAGSSVPAKGLFLVTVNYKEDIYID"
     gene            complement(2187..3056)
                     /locus_tag="PKH58_09215"
     CDS             complement(2187..3056)
                     /locus_tag="PKH58_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89250.1"
                     /translation="MNKHFFFIISMFFALTSGLNAQDTIVLKNGTQILSKISEIGTNG
                     IKYKAQENPDGPDYVLEKFKVHEIRFHNGFVQQYNVNEENPSTDDIEYINDVPVKKHI
                     EIREERFVNEPRNEDIRTEPVQMGQKYKINKLYYNSSMYVPHPGDPYNPSVAGIASFF
                     VPGLGQMLSGEIGRGFGFLGGYAASYLVCLSGLAIAQGEYYYDYDSYPSPNYNNNSEI
                     GVGLFIVGGVSMIATYIWSIVDAVKVAKVNNMYMQDLRKNQVNIQLKPYMETKKYPGI
                     TDRPIGMTLQINF"
     gene            complement(3137..4156)
                     /gene="pheS"
                     /locus_tag="PKH58_09220"
     CDS             complement(3137..4156)
                     /gene="pheS"
                     /locus_tag="PKH58_09220"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNX89251.1"
                     /translation="MKTKIQQLLEEVNSLKASTLEEAEALRIKYLSKKGEITNLFNDF
                     RNVPNEEKREVGQLLNELKNNAQEKLNALKEVLTASGKTEQKTDLTRTAEPLKLGTRH
                     PLSLVKQEIADIFSRIGFTIAEGPEIENDWNVFGALNFPPEHPARDMQDTFFIEKNPD
                     ILLRTHTSSVQTHVMSSQKPPIRMIFPGRVYRNEAISYRAHCFFHQVEGLYIDKNVSF
                     ADLKQALLYFAKEMFGPDTKIRLRPSYFPFTEPSAEMDISCNLCGGEGCAFCKHTGWV
                     EILGCGMVDPNVLDNCGIDSKIYSGYAFGMGVERITNLKYQVKDLRLFSENDVRFLEE
                     FTSAF"
     gene            4354..5022
                     /locus_tag="PKH58_09225"
     CDS             4354..5022
                     /locus_tag="PKH58_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNX89252.1"
                     /translation="MKNILDFLSELKENNDRTWFNEHKNEYESARKEFEQICYSLILK
                     IGVFDEDIKHVDVKDCVFRIYRDIRFSNDKTPYKTHFGAFIASAGGRKSNRGGYYLHL
                     DPAGSFLSVGVWCPPPAVLKAIRQSIYYNIEEFNEIRSDKDFIRLFKNSFYEEDKLKT
                     VPQGFSKDFKDAELLKLKHFLVSYDLDQNMLFSNDFVSEIASIAEKSYPFIRFLNYTV
                     DEVV"
     gene            5047..7056
                     /locus_tag="PKH58_09230"
     CDS             5047..7056
                     /locus_tag="PKH58_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNX89253.1"
                     /translation="MKSSKMLPLVASDPWLEPYSHAIQGRYDYFLSVEKRLTGESGCL
                     SDFATGYLYFGLHHTPDGWIFREWAPNATAIYMIGDFSGWVKKPEYRLNRIENDVWEI
                     KLPENALKHLQLYKLVVEWEGGFGERIPAWSKRVVQDEHTKIFSAQVWNPENPFQFKV
                     DKFVPDTKPLLIYECHIGMSSPEEKVSTYEEFRTNILPRIARDGYNCIQIMAIQEHPY
                     YGSFGYHVSSFFAPSSRFGTPDDLKRLIDQAHEIGIAVIMDIVHSHAVKNEVEGLGRF
                     DGTPYQYFHGGSRREHPAWDSLCFDYAKPAVLHFLLSNCKYWLDEFKFDGFRFDGVTS
                     MLYRSHGLGEDFSGYSSYYNLNQDGDAICYLTLANKLIHEVNPNAITVAEEVSGIPGL
                     AAKINDGGFGFDYRMAMGIPDFWIKYIKEVKDEEWKAGHIFWELTNRRADEKTISYAE
                     SHDQALVGDKTIIFRLIDADMYWHMQRGDENFRVVRGVALHKMIRLITATTINGGYLN
                     FMGNEFGHPEWIDFPREGNGWSYKYARRQWNLVDNQNFIYCGLGEFDRAMIETITSVK
                     AFNETPIIKIWDNEGDQIIAYQRDKMIFVFNFNGTKSFADYGILAESGSYKVILNTDN
                     PVFEGHGLIDESIEHFTCKGPGDMPGKEWLKLYIPARTAFVLKKQ"
     gene            7198..7665
                     /locus_tag="PKH58_09235"
     CDS             7198..7665
                     /locus_tag="PKH58_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89254.1"
                     /translation="MNDQVLNEAKKQYAVDMHTAEHILNQTMVRMFGCGRSMNAHIER
                     KKSKCDYFLPVSPTENQITEIISRVNEIINKHLPVSEVIMTRDEASRIVDLSKLPDDA
                     SEMLRIIKVGDYDACACIGQHVQNTEEIGPFEILSHDYENGRWRVRWRVTEKN"
     gene            7677..8357
                     /gene="mtgA"
                     /locus_tag="PKH58_09240"
     CDS             7677..8357
                     /gene="mtgA"
                     /locus_tag="PKH58_09240"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNX89255.1"
                     /translation="MKKIGKILRNLVILFFASSIFAVLVFRFIPVFITPLMVIRSVES
                     ITDGEVPKIKKTWVPLEDISQNMVQAVVASEDNLFMEHNGFSVTDIKKAISHNKKGKR
                     IRGGSTISQQTAKNVFLWPDRSWVRKGLEAYFTVLIEFLWSKERIMEVYLNVIETGDG
                     IYGVQAASKVYFGKKASKLSRSQAALIAVCLPNPRKFNPADPSGYILRRKNHIVNLMG
                     KIERVNFE"
     gene            8372..9058
                     /gene="lipB"
                     /locus_tag="PKH58_09245"
     CDS             8372..9058
                     /gene="lipB"
                     /locus_tag="PKH58_09245"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNX89256.1"
                     /translation="MITKMNFEDWGLIEYNQAWEKQEKLFNVTIQQKIKELPTQNYLI
                     FCEHPHVYTLGKSGNENNLLLDYIQLQAKNATFVHTNRGGDITYHGPGQLVGYPIFDL
                     EKYNIGLKQYIYNIEEAIIKCLAEYNIICERLEKATGVWMDVGKPDCRKICAIGVRSS
                     RFVTMHGFALNVNTQLEYFSYINPCGFIDKGVTSMQKELGAAVDMASLKTNLKLHIEK
                     QFQQVQKETP"
BASE COUNT         2836 a         1625 c         1833 g         2937 t
ORIGIN      
        1 cgggatatgt tccgttaggc aatgatgtgg tgtatgatgt gggagtataa acttcaattc
       61 tcatcaggtc gccgctaccg ctcatatttg tcatgttaat attctgatca gcaagccaga
      121 tggtgtaaat attcaagcct acattatcta tatcgcccca gtaccagagt tgcccttttg
      181 taagagtttc aggataggga gttacaaggt cggtagttgg aatatttccg ctgtatgaga
      241 actcataaaa ttcattatca ctggtagtaa cctgtccgct gatgctgtaa ttacccattt
      301 tattgacaat gatactaccg cttgacaaaa acaggtaatc tgtaattgag ccatttttta
      361 cgattgcaaa ataacttccg gttggaactt cttcattatt gttgttcaaa cccatttttc
      421 ctctctggaa gctgtattcg atattttcgt tcgaattctc gttgttcacg ttatacgtac
      481 cggcaggtat gctggtggca ttgaaattcg tattcatgtc aagaaacagg aatgttccgc
      541 tgccggtgat gtatccttca gcgtcagtcc ctaaattctc atcaaaaaaa taggcttcaa
      601 tagcattgac attgttgcgg taaatattcc ccaggtaatt agctttagcc cgggtaaagt
      661 tgattttgtt gttttctacc tcgggttttt tttcgcaagc tgagaatgct gcagcaacac
      721 tcaataatat tactgatatt tttataaact gtttcatggt tgaatgtttt aatggtttat
      781 atcacttttt tattttctga gattaaatca aaaattgtgc ctgcttttga aaaaagcatt
      841 tctcaggact aataaaaata aaaaaacccc gaatattcgg ggttaaatta ttgattgtca
      901 gcaaaaagaa aaatttattt tttttctccg cgattgccgt aacgactcat gaatttgtca
      961 acacgtcctg cagtatcaac cagtttcgat ttacctgtat agaaaggatg tgatgaactt
     1021 gagatttcta actttaccaa tggataggtt actccgtcaa tatcaacagt ttcttttgtg
     1081 tttactgttg aacgggaaat aaatgtttcg ccgttggaca tgtctctgaa tgctacaggg
     1141 cgataatttt ctggatgaat tccgcttttc atatcttaat ttttttattt tatttctttt
     1201 gattttggct tgcaaaatta caaataataa tcaaagtatc aaattatttc cggaaatatt
     1261 tattcaaaaa tcattaagaa acaaccccga agtgatgctt tgtgctgtta agtaattgta
     1321 tttcagtcga atatcgcagt ttttatcagt cgatatatat gtcttcctta taatttactg
     1381 taactaggaa taatccttta gccggaaccg aactgcctgc atcacagcga ttttttgctt
     1441 caatgatttt tctgaagtct tcgatggtca tttttcctct tccgacttct aacagtgtac
     1501 ctacaattgc acggaccata tttcttaaaa aacgatctgc ttcaatagta aaaatccatt
     1561 cctcacctct ttgctcccaa tgagcatgct taatcttgca gttgttggtt tttgtatcgg
     1621 tgtgaagttt gctgaaactg gtaaaatctg tataatcaaa aagtgttttt gctgctacat
     1681 tcatcctgtc aaaatctaaa ggatgataaa tccgcatggc aaattcatgg cgaaacacgt
     1741 ccttatgaag cagaatatga tactcgtatc gccgtgaaat ggcatcgaaa cgggcatgga
     1801 catcaggttt cacttcccgg atattcctga ctgatatatc ggagggtaaa aaactgttca
     1861 atttactaac cagatccatt tcgactggaa aatctccttc gaaatcgaaa tgtgccacca
     1921 tcggacgtgc atgtacaccg gcatcggttc tgccagctgc aaccagttca atttcggttc
     1981 gcagtatggt gcataaagca ttggtcagta cgtgttgtac cgataatccg ttaggctgta
     2041 tctgccatcc gcagtaaccg cttccgttgt agtcgaacca gataaaatat cttcttttca
     2101 cactgcaaag ataatgtttt gcttgcatac aaaaaaagca ttgacttttt cgccaatgct
     2161 tttcaaatca gttttaaata atccggttag aaatttatct gtaaggtcat acctatcgga
     2221 cggtcggtga taccgggata tttttttgtt tccatatagg gtttcagctg tatattcacc
     2281 tgattttttc ttaaatcctg catatacatg ttatttacct tggcaacttt taccgcatca
     2341 actatcgacc aaatatatgt tgctatcatg gatacaccgc caacaataaa taatccaacc
     2401 cctatttcgc tgttgttatt gtaattaggg gaggggtaac tgtcataatc atagtaatat
     2461 tcgccttgtg ctattgccag gccggataag caaaccagat agctggcggc ataaccaccg
     2521 agaaatccaa atccgcgacc aatttcacca ctgagcatct gaccaaggcc ggggacgaaa
     2581 aatgatgcta ttccagccac cgaaggattg taagggtcac cgggatgggg gacgtacatg
     2641 gatgaattgt aatataattt gttgatttta tatttttgtc ccatttgtac aggctctgtc
     2701 cttatgtcct cattccgggg ttcattgaca aatctttctt ccctgatttc aatgtgtttt
     2761 tttaccggaa catcattgat gtattcaata tcatctgttg aaggattttc ttcattcacg
     2821 ttatattgtt gtacaaatcc attgtgaaaa cgaatttcgt ggactttgaa tttttcaagt
     2881 acatagtcag ggccgtcggg attttcctga gctttgtatt taattccatt cgtaccaatt
     2941 tccgatatct ttgagagaat ttgagtgccg tttttaagaa caattgtatc ctgagcattc
     3001 agtccggatg tcaaggcaaa aaacattgaa ataatgaaga aaaaatgttt attcataaga
     3061 gactacttta atggtttatg attatataat gtatgtaaac tctcaaatta ttgatttctg
     3121 aaaagaaact acatccctaa aatgcgcttg tgaattcttc caggaatcgt acgtcgtttt
     3181 ccgagaacaa gcgaaggtct ttaacctgat atttaaggtt ggtaatgcgt tctaccccca
     3241 taccaaaggc ataaccggaa taaatcttcg aatcgatgcc gcagttatcc agtacattcg
     3301 gatcaaccat accgcatcca aggatttcga cccagccggt atgtttgcag aaagcgcagc
     3361 cttcgccacc gcacaaatta cacgaaatat ccatttcggc acttggctcg gtaaatggga
     3421 aataggaggg acgcagacgg attttggtat ccggcccgaa catttccttt gcaaaataaa
     3481 gcaaagcctg tttcaaatca gcaaacgaaa catttttatc aatataaagt ccttctacct
     3541 gatgaaaaaa acaatgcgcg cggtatgaga tggcttcgtt gcggtaaaca cgtccgggga
     3601 aaatcatccg gataggcggc ttttgagagg acatcacgtg tgtctgaacc gatgaagtat
     3661 gagtacgaag caaaatgtcc ggatttttct caataaaaaa tgtatcctgc atatcgcggg
     3721 cagggtgctc gggtggaaaa ttcagagcac cgaacacatt ccagtcattt tcaatttctg
     3781 gtccttcggc gattgtaaat ccgatacggg aaaaaatatc tgcgatttcc tgttttacca
     3841 aagaaagtgg gtgccgtgta ccaagcttca gcggttctgc cgtgcgggtc agatcagttt
     3901 tttgttcggt ttttcctgat gcggttaata cttctttcag ggcgttgagt ttctcttgag
     3961 cgttgttttt taattcattc aataattgtc cgacttcacg tttttcttca ttcggtacat
     4021 tacggaaatc attgaaaaga ttagtaattt ctcctttttt gcttaagtat ttgatacgta
     4081 atgcttctgc ctcttccagt gtacttgctt tgagagaatt cacttcttcc aataattgct
     4141 gaatttttgt tttcatctga tgtcttaaaa atatatagtt attgttcgtc tgatagttat
     4201 tgaattatta ttcagagtat ttctccggaa taatttacaa aagtaagcat ttttcactaa
     4261 ttctgaagtt tgaaatataa aaatctatac aggatgctct tttaatacta aatttttgac
     4321 tatttttgta acaaattaat taagtgaaac aggatgaaaa acatattgga ttttctttca
     4381 gagcttaaag aaaataatga cagaacctgg tttaatgagc ataaaaacga gtacgaatca
     4441 gcgcgtaagg aatttgaaca aatctgttat tctctgatac tcaaaatagg tgtgtttgat
     4501 gaggatatca aacatgttga tgttaaagac tgtgttttcc ggatttaccg ggatattcgt
     4561 ttttcaaacg ataaaacacc atataaaaca cattttggag catttattgc atctgccgga
     4621 ggccggaaaa gcaatcgcgg tggttactat cttcatcttg atcctgcagg tagcttttta
     4681 tcggttggag tttggtgtcc gccacctgcg gtattaaaag ccattcgtca aagcatttat
     4741 tataatattg aagagtttaa tgaaatcaga agcgataagg attttataag attattcaaa
     4801 aattcttttt acgaagagga taagcttaaa actgttccac aaggtttttc aaaggacttt
     4861 aaggatgcag agttgcttaa attaaagcat tttttggtat cttatgattt ggatcaaaat
     4921 atgctttttt cgaatgattt cgtgtctgaa attgcttcaa ttgccgaaaa atcatatcct
     4981 tttatcaggt tcctgaatta tacagtggat gaagttgttt aataaaaaaa atatatcact
     5041 aaacatatga aaagttccaa aatgttgcct ttagttgcat ctgatccctg gcttgaacca
     5101 tattctcatg caattcaagg aaggtatgat tattttctgt ccgtagaaaa acggttgaca
     5161 ggtgaaagtg gatgtctttc tgattttgca accggttatt tgtattttgg actacaccat
     5221 actcccgatg gctggatttt cagagaatgg gctcccaatg ctacagcgat ttatatgatt
     5281 ggtgattttt ctggctgggt gaaaaaacct gaatatcggt taaatagaat agaaaatgac
     5341 gtctgggaaa taaaattgcc agaaaatgca ttgaaacatt tacaattgta caaactcgtt
     5401 gtagagtggg aaggcggttt tggagaacga ataccagcct ggtctaaaag ggttgtgcaa
     5461 gatgaacata ccaaaatttt cagtgcacag gtttggaatc ctgaaaatcc atttcagttt
     5521 aaggttgaca aatttgtacc cgatacgaag ccattactga tttatgaatg ccatattggc
     5581 atgtcaagtc cagaagaaaa agtttcaacc tacgaagaat tcagaaccaa catattgcca
     5641 cgaatagcca gagatggtta taattgtatt cagattatgg ctattcagga acatccttac
     5701 tacggctcat ttggatatca tgtctcaagt ttttttgccc catcatcccg gtttggcact
     5761 cctgatgacc tgaaacgact aattgatcag gctcacgaga taggaattgc agtcataatg
     5821 gatattgtcc attcacatgc agtgaaaaat gaagtggaag gattgggacg ctttgacgga
     5881 actccctatc aatattttca cggaggcagt cgtcgtgaac atccagcgtg ggattcattg
     5941 tgtttcgact atgcaaaacc cgcagtattg cattttctgc tgtctaactg caagtattgg
     6001 ctcgatgaat ttaaatttga tggtttccgg tttgacggag tcacttccat gttataccgc
     6061 agtcatggat tgggtgaaga tttcagcgga tacagttcat attacaatct caatcaggat
     6121 ggtgatgcaa tttgttacct tacactcgct aataaactta tacatgaagt taatccaaat
     6181 gcaataactg ttgccgaaga agtgagcgga attccggggc tggcagcaaa aataaacgat
     6241 ggtggatttg gattcgatta tcgcatggca atgggcatac ccgatttctg gataaagtat
     6301 attaaagaag taaaggatga agagtggaaa gccggacata ttttttggga attgactaac
     6361 cgcagggctg atgaaaaaac catcagttat gctgaaagtc acgatcaggc tcttgttggc
     6421 gacaaaacta taattttccg tctgattgat gccgatatgt attggcatat gcaaaggggt
     6481 gatgaaaact tcagggttgt acgaggtgta gctcttcaca aaatgatacg gctgattaca
     6541 gccacaacga ttaatggtgg ttacctcaac tttatgggca atgaatttgg tcatcccgaa
     6601 tggatagatt ttccgcgcga aggtaatggc tggtcataca aatatgcacg cagacaatgg
     6661 aatttggttg ataatcaaaa ctttatatat tgtggactcg gagaattcga ccgtgccatg
     6721 atcgaaacta ttacatcggt taaagccttt aatgaaactc caatcataaa aatctgggac
     6781 aacgaaggtg atcagataat tgcatatcag cgtgataaaa tgatattcgt ttttaatttt
     6841 aacggaacaa aatcatttgc tgattatgga atactcgctg aatccggaag ttataaggta
     6901 atacttaaca cggataatcc tgtctttgaa ggacatggat tgattgatga aagtatcgaa
     6961 cattttacat gcaaaggtcc cggagatatg ccaggtaagg agtggctgaa actttatata
     7021 ccggcacgaa cagcctttgt acttaaaaag caataatctt caagttatca gtaataaaac
     7081 gagtctgatg cagtttgaat actgtatccg actttttatt ttataaagaa ctgatattta
     7141 tggcaacata taagtgtgaa ttattaattt tgcatccatt tgtaaatata tgaatttatg
     7201 aacgatcaag tattaaatga agctaagaaa cagtatgctg tggatatgca tactgcagaa
     7261 cacattctga atcagacaat ggttcgtatg tttggatgcg gacgatccat gaacgcgcac
     7321 atcgaacgaa aaaaatccaa gtgcgactac tttttaccgg tatcgcccac agaaaatcag
     7381 ataacggaaa ttatatcgcg ggttaatgag ataataaata aacatttacc agtaagtgaa
     7441 gttataatga cccgggatga agcctctaga attgttgatt tgtcaaaact gcctgatgat
     7501 gcttccgaaa tgcttcggat aattaaagtc ggtgattatg atgcctgtgc ctgtattggt
     7561 cagcatgtac agaataccga agagataggt ccttttgaga tattaagtca tgattacgaa
     7621 aatggcaggt ggcgtgttcg ttggcgtgtt acggagaaaa attaaattga actgaaatga
     7681 aaaagattgg aaaaattctg cgtaatctgg taatcttatt ttttgcatca agcatttttg
     7741 cagtgcttgt ctttcgcttt attccggttt tcattacacc tctcatggtt attcgcagtg
     7801 tcgagtcgat tacagatggt gaagtaccta aaataaaaaa aacctgggta cctctcgaag
     7861 atatatcaca aaacatggta caggcagtgg ttgcttctga agacaacctg tttatggaac
     7921 acaatggctt ttctgttact gatattaaaa aagcaatatc gcataataaa aaaggtaaac
     7981 gtataagagg aggaagtaca atatcgcagc aaacagctaa gaatgtcttt ctatggcctg
     8041 acaggtcgtg ggtaagaaaa ggtcttgaag catattttac tgtactgatc gagtttttat
     8101 ggtcaaaaga gcgaattatg gaagtatatc tcaatgtgat agaaacaggt gatggtattt
     8161 atggggtcca ggcagcttcc aaagtttatt ttggaaaaaa agcatcgaaa ctatcacgtt
     8221 cgcaggcagc attgattgcg gtatgtttgc ccaatccgcg caaattcaat ccggcagacc
     8281 cttcaggata tatattaaga agaaaaaatc atattgtgaa cttaatgggg aaaatagaga
     8341 gagttaattt tgaataataa gcaccagaca tatgattact aaaatgaatt ttgaagattg
     8401 gggtttgatt gaatataatc aagcctggga aaaacaggaa aaattattta atgtaactat
     8461 tcaacagaaa ataaaagaat tgcctacaca aaattacctg attttttgcg aacatccaca
     8521 tgtttacacc ttaggcaaga gcggaaatga aaacaatttg ttacttgatt atattcagtt
     8581 acaggctaaa aatgcaacat ttgttcacac caaccggggt ggagatatca cctatcatgg
     8641 tcctggtcag ttggtaggct atccaatttt cgatcttgaa aaatataaca taggattgaa
     8701 gcaatacata tataatatag aagaggcaat catcaaatgt cttgcagaat acaatattat
     8761 atgtgaacgc cttgaaaaag caaccggtgt ctggatggat gtcggaaaac ctgattgccg
     8821 aaagatatgt gcgataggtg tacgtagttc tagatttgtt actatgcatg gttttgctct
     8881 caatgtcaat acacagctcg aatatttcag ttacattaat ccatgcggat ttattgacaa
     8941 aggtgttact tcaatgcaga aagaattagg tgcagctgtc gatatggcat ccttaaagac
     9001 aaacttaaag cttcatattg aaaaacaatt tcaacaagtt cagaaagaaa ctccataatt
     9061 gagacttctt cttggtgtta tgtacgataa tttacttaca tttttatagc gtttagatgt
     9121 cataaaatca gactattatg gatttgtccg aaaatatatt tgaaaattgc ttatagttta
     9181 tttgttcact ttaaaaactt tgtttacctt tgcacccgct ttgagagaga a
//
LOCUS       DAOPYB010000119         9123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_8981_length_9123_cov_10.881341, whole genome shotgun sequence.
ACCESSION   DAOPYB010000119 DAOPYB010000000
VERSION     DAOPYB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8981_length_9123_cov_10.881341"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..986
                     /locus_tag="PKH58_09250"
     CDS             387..986
                     /locus_tag="PKH58_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89257.1"
                     /translation="MKKTTFTLLCLIIIFCYNNKIIAQVQSDSTSATLEETLSWIKSK
                     VDGKKYNISYEYVSCTDNKSKIVSTTTIKTLILFSYDIKNLSFTVTSKYTTIWSTSDS
                     ITQPNSFTLYLSDINAMSLVCFKDTVYRETREYENTDQKGNEKQICDITNNMYRYCSM
                     YFRANNQKFEMIIPDEFIYDRLLKAFNHAVDLVKKTEKF"
     gene            complement(1239..1541)
                     /locus_tag="PKH58_09255"
     CDS             complement(1239..1541)
                     /locus_tag="PKH58_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89258.1"
                     /translation="MALDKEFFDSLEKLKNFKTKPELNDFFEVINKLERIGDEIDVED
                     KETITDSATLIKQAKDLTKKLDVNRDEFAFRTKTQKEFDAVYECIDYLGEHFNSLN"
     gene            1980..3293
                     /locus_tag="PKH58_09260"
     CDS             1980..3293
                     /locus_tag="PKH58_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX89259.1"
                     /translation="MKDTPINYDVVKRQIAASNIPNVGKATIREVKRLINEIEKETGQ
                     KFVRMEMGVPGLPATQIGIDAEIQALKNGCAAIYPEIDGMPELKREMARFVKLFLDTE
                     VTPENCIPTVGSMQGGYAAFMTVNRMYKEKDTTLMLDPCFPVHKQQHAVIGTKFEAFD
                     VYDYRGDKLKDKIESYLKKGNIASIMYSNPNNPSWICFTDKELKIIGDLANKYNVLVL
                     EDLAYFGMDFRKDYSKPGVPPFQPTVAKFTDNYILFVSGSKVFSYAGQRIGMMVISPK
                     VFNTHAPDLKRYYASDLFGRAMVYGTIYSLSAGTAHSVQYAMTAMLKAANDGIFNFVD
                     PVMEYGEKAKIMKKMFTDNGFNIVYDKDENDPLADGFYFTFSYPGFTGEQLLEELMYY
                     GISAISLTITGGRAEGMRACVSLVQRSQFPDLEYRLKKFNEHHPL"
     gene            3353..3586
                     /locus_tag="PKH58_09265"
     CDS             3353..3586
                     /locus_tag="PKH58_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX89260.1"
                     /translation="MAKLKGAIVINIERCKGCEVCTTACPEDVIVMNPGVNAKGYHYA
                     MMGNDNCVGCANCAVVCPDGVITVYRVKVATEV"
     gene            3606..4691
                     /locus_tag="PKH58_09270"
     CDS             3606..4691
                     /locus_tag="PKH58_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNX89261.1"
                     /translation="MMKELRLMKGNDAIAEAAIRCGTDGYFGYPITPQSEILEYLMDQ
                     KPYETTGMVVLQAESEVAAINMVYGGASCGKKCMTSSSSPGISLKQEGISYIAGAELP
                     CLIVNVVRGGPGLGTIQPSQGDYFQATKGGGHGDYRLIVLAPASVQEMSDFVSLGFEL
                     AFKYRNPTMILSDGVIGQMMEKVELQETKPRWTKEEIVKMSPWATTGKTPDRERNIIT
                     SLDLDPARQEKHVMKLDRKFKEIEANEVRYEKINCDDAEYLFVAFGSSARICQKSMDI
                     ARAKGIKVGLLRPITLWPFPYKPLFELAGQVKGMLTVEMNLGQMVEDVRLAVNGKVRV
                     EHYGRVGGIIPTPDEIVEVLETKIIGG"
     gene            4704..5471
                     /locus_tag="PKH58_09275"
     CDS             4704..5471
                     /locus_tag="PKH58_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX89262.1"
                     /translation="MTREQIIKPENLVYKRTKLLTDNVMHYCPGCGHGTAHRMVMEVV
                     EEMGIQAETIGVAPVGCSVFAYDYMNIDMQEAAHGRAPALATAIKRLMPEKYVFTYQG
                     DGDLAAIGTAETIHACNRGENIVIIFINNGIYGMTGGQMAPTTLPGMKASTCPFGRDV
                     AIMGNPLKMTEIVATLPGTCFVTRHSVHTPANVRKAKRAIRKGFELSHAGKGVSFIEI
                     VSNCNSGWKMTPVQANKWMEENMFPFYPLGDIKVPAE"
     gene            5532..6083
                     /locus_tag="PKH58_09280"
     CDS             5532..6083
                     /locus_tag="PKH58_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNX89263.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGVMQNKEVSWMPSYGPEMR
                     GGTANVTVIVSDERVSSPILTVFDTAIILNQQSMDKFEKSVKPGGLLIYDGNGITRHP
                     ERKDINVYRVDAAEEASKLGMNKVFNMMVLGAYLKIKPIISPEFIRKGLEKSLPARHH
                     GLIPDNEKAIEIGKEIIREVHKV"
     gene            6169..7113
                     /locus_tag="PKH58_09285"
     CDS             6169..7113
                     /locus_tag="PKH58_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNX89264.1"
                     /translation="MKIPDKKIIEEAACRIRQAVAHTPVMTSQSLDEMFQAHLYFKCE
                     NFQKAGVFKSRGACNAVFSLNENQLDRGVAAHSSGNHAQALARAAKLRNTKAYLVMPE
                     NSSEVKIAAVKAYEGIITFCKPALEARETTLKEVISKTGAAEIHPYDNYQVIAGQATV
                     TKELVGDISALDYIICPVGGGGLLSGTLLAAKYFSPHTKVIAAEPEQANDAYLSFKSK
                     IFIPSVNPDTIADGLRTSLGTLTFPIIMEHLHDIFTVSENDIIASMKLIWERMKIIVE
                     PSSAVTLAVLKNNPGLFKEKKTGIIISGGNVDIKNLPW"
     gene            complement(7120..7848)
                     /locus_tag="PKH58_09290"
     CDS             complement(7120..7848)
                     /locus_tag="PKH58_09290"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX89265.1"
                     /translation="MKPRLFLTLALILTGLVANAQTVSKNPISLGMNAPEFKSLSTQG
                     EINFPADYYNKWKVLFSHPADFTPVCTSEIIALADKQEEFKYLNAALIVISTDGLNSH
                     IEWVKSMEGITRTGKSAIKINFPLVADADLTVVKKYNMMHQDSTNRESVRSVVVIDPD
                     NKVRAFFVYPDNIGRNIDEILRTLKALQMADKDNVLTPANWQIGKDVLIHAPKSMKES
                     EKLKLQNNPNMYSLTWYMWYKKDQ"
     gene            8012..8170
                     /locus_tag="PKH58_09295"
     CDS             8012..8170
                     /locus_tag="PKH58_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89266.1"
                     /translation="MKLIWERMKINVKHSSAVTMALIKNNPGLFKEKRTGIILSGGNV
                     DMTKLPWL"
     gene            complement(8178..8771)
                     /locus_tag="PKH58_09300"
     CDS             complement(8178..8771)
                     /locus_tag="PKH58_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89267.1"
                     /translation="MKKVLILAAFVIFAGIASTSFAQKHEVKSTWFKNGSVLTYHVQN
                     VTKEYDYIVSDLVLGLDVAFKWKMTDPVNYKGNITIYDAAIDTAMKMVNYFENNSSLN
                     LTNSTTAMFSRKAYKQLISQQSINVTVDKDKETIIFDRYENYPIEVDGVKQDVEVMVA
                     KSESGKKFWILDNPKYPLILKMELAFTIDLRSVVTAK"
BASE COUNT         2848 a         1706 c         1852 g         2717 t
ORIGIN      
        1 gcgagttata tagatttgaa aaataatata gtccaaactt catttcaatt gtttgcacta
       61 tttatttttc tatcggcata accaaagtaa gaatttgtag tacgtctttg cttcggcaag
      121 ctcagcaacc atggactatc tacaaattca ctttggtatt agtaataaga ttttgatttt
      181 tcccatattt gagtaggagt ttccggggct tcataattaa aaaatattta ttttattttg
      241 cattttagtt tagtttttac tatatttgta tttaatattg aaaacttaaa aattatttaa
      301 cactatcagg ttgagattgt aaattgataa attaaaatat agcatgactc aaaaacatta
      361 ttataaatta tttaaaaaat gcaactatga aaaaaacaac atttacgtta ttgtgcctca
      421 taataatttt ttgttataat aacaaaataa ttgcgcaggt acaatccgat tcaacttccg
      481 ctactttaga agaaacatta agttggatta aaagcaaagt tgatggtaaa aaatacaata
      541 tcagttatga atatgtctcc tgtactgata ataaatccaa aattgtaagc acgacaacca
      601 tcaaaacgtt aattcttttt tcatacgata ttaaaaacct ttcttttaca gtgacttcaa
      661 aatacacaac aatctggtca accagcgatt ctattacaca gcctaattca tttacactgt
      721 atttgtcgga tataaatgcg atgagtcttg tttgtttcaa agatacggta taccgcgaaa
      781 cgagagaata tgaaaataca gaccagaaag gtaatgaaaa gcaaatatgc gatattacca
      841 ataacatgta caggtattgc agtatgtatt tcagggctaa taaccaaaaa ttcgaaatga
      901 ttattcctga tgaatttatt tatgaccgtc tgctaaaagc gtttaaccac gctgttgact
      961 tggttaaaaa gacagaaaaa ttttagatta atacttgtta caggcctctg tgataaagtc
     1021 ttataccaag gtgataaagt atgagtttaa aatagcgatg agagtaaaat aatctatttt
     1081 ccttcaatta tataacctca gggggttgtg atttgaattt atcaaatcac aaccccctga
     1141 agtttatttt tcgtaaatgg aaaaagcatt agtatccctg tctggctgag gttttgccgt
     1201 tgtctgtttt gatttctttt ttgactgtct ggaatgtatt aattcaaaga attaaagtgt
     1261 tcgcctaaat aatctataca ctcatacact gcatcaaatt ccttttgagt ctttgtgcga
     1321 aaagcaaatt cgtctctgtt gacatctaat ttctttgtaa ggtcttttgc ctgttttatc
     1381 agggttgctg agtccgtaat tgtttccttg tcctcaacat ctatttcatc acctattctt
     1441 tcaagtttat taataacttc aaaaaaatca tttaactcag gtttggtttt gaagttttta
     1501 agcttctcta aactgtcgaa aaattctttg tcaagtgcca tagtggtatt ttttatatgt
     1561 tactaaattc cattacagta atttgtgtaa aaaacgatga tttctaatca ggggcttatt
     1621 acacctcaaa ataccataaa ttgctgttta tcaaaagttt atttattata cggtattttg
     1681 aaactgcttt tcaccacaaa tgtaggtgtt atattttaaa aaaacaaata aaatatgaaa
     1741 aattattgat aaataatatt tttttcaaga taaaataaaa ttgctgtttt ttcgttatcc
     1801 aatcctctgg tcgaattaga acaagttcat ttctatgtgt tttaggctgt tatcattaca
     1861 agattttttt aacttgtcac ccggccaaat aaatttgctt taatctttgt tttttaggaa
     1921 aataaatcgg ttatttgcag attagaaaat gccaaaacca taagaaaaat taatatacta
     1981 tgaaagatac acctattaat tatgatgttg ttaagcgtca gattgctgcg agcaatatcc
     2041 ctaatgttgg caaggccact atacgcgaag taaaaagatt gatcaacgaa attgaaaagg
     2101 agaccggtca gaagtttgtc aggatggaga tgggtgttcc cggtttgcct gctacacaaa
     2161 taggcattga tgccgaaata caggcattga aaaatggctg tgccgcgatt tatcccgaaa
     2221 ttgacggtat gcctgaactt aaaagagaaa tggcgcgatt tgttaagtta ttcctcgata
     2281 cagaggtaac tcctgaaaac tgtattccta cggtgggttc gatgcaggga gggtacgcgg
     2341 cattcatgac cgttaaccgc atgtacaaag aaaaagacac cacgctgatg cttgacccgt
     2401 gttttcctgt ccataaacag caacatgccg ttatcggcac caaattcgaa gcttttgatg
     2461 tatatgacta ccgcggcgat aaactaaaag ataagataga gtcctatctg aagaagggga
     2521 atattgccag tatcatgtat tccaatccaa acaatccctc atggatctgc tttacggata
     2581 aagaattaaa gatcatcggc gacctggcga acaaatacaa tgttttagtt ttggaagacc
     2641 ttgcctattt cggcatggat ttcagaaaag attattcgaa accgggagtt ccgcctttcc
     2701 aacctacggt ggcaaagttt accgacaatt atattttgtt tgtttcagga tccaaagtgt
     2761 ttagctatgc aggacaacgc atcggcatga tggtgatctc cccgaaagtg tttaacaccc
     2821 atgcgccgga cctgaaacgt tattacgcat cggatctgtt tggccgggct atggtttatg
     2881 gaaccatata ttctttgagt gcaggaacag cccatagtgt tcagtatgca atgacagcca
     2941 tgctcaaagc ggccaatgat ggcattttca actttgtgga cccggtgatg gaatacggtg
     3001 aaaaagcaaa gattatgaaa aagatgttca ccgacaatgg gttcaacatc gtttatgata
     3061 aagacgaaaa cgatcctttg gccgacggat tttattttac cttctcttat cccgggttca
     3121 ccggagagca actgcttgaa gaattgatgt attatggaat aagtgccata tcgcttacca
     3181 taacaggtgg ccgtgcagaa ggcatgcgcg cctgcgtatc tttggttcag cgcagccagt
     3241 ttccggatct ggagtaccgc ctgaaaaaat tcaacgaaca tcatccgctg taatctgact
     3301 aatattagct aatttcgtgt tttaattaat aattctaaaa tcataaataa aaatggcaaa
     3361 attaaaagga gcaatcgtaa ttaatatcga acgctgtaaa ggatgtgaag tctgcaccac
     3421 agcgtgtccg gaagatgtga tcgtgatgaa tcccggtgtt aatgcaaaag gatatcacta
     3481 tgcaatgatg ggcaatgaca attgtgtcgg atgcgcaaac tgcgctgtag tatgccccga
     3541 tggtgtgatc actgtgtaca gggttaaagt cgcaactgaa gtttaataat tttaaaaaag
     3601 tataaataat gaaagaacta agattaatga aaggtaatga cgcgatagct gaagctgcaa
     3661 ttcgttgtgg tactgatggc tatttcggat atcccattac tccccaatca gaaatacttg
     3721 aatatcttat ggatcaaaag ccatacgaaa ccaccggaat ggttgtgctt caggccgaaa
     3781 gcgaagtggc tgccatcaat atggtgtatg gtggtgcttc ctgtggaaaa aaatgcatga
     3841 catcatcatc cagccccggt atcagtctta aacaggaagg tatttcatat attgcaggcg
     3901 ccgaactgcc ctgcctcatt gtcaatgtag tgagaggtgg tcccggatta ggtactatcc
     3961 aaccttcgca gggcgattat tttcaggcta caaaaggcgg cggacacggc gattatcgtt
     4021 tgatcgtgct ggctccggct tctgttcagg aaatgtctga ttttgtctct ttaggatttg
     4081 aacttgcctt taaataccgt aacccgacga tgatactctc tgacggagtt atcgggcaga
     4141 tgatggaaaa ggtggaatta caggaaacta aaccccgctg gaccaaagaa gaaattgtga
     4201 aaatgtcccc ctgggctact acagggaaaa ctcccgaccg tgaaaggaat atcatcacct
     4261 ctttagacct tgaccctgca aggcaggaaa aacatgtgat gaaattagac cgcaaattca
     4321 aggagataga agcaaacgaa gtgcgttatg aaaagatcaa ttgcgacgat gctgaatatt
     4381 tgttcgtggc ctttggttcc agcgcacgta tctgccagaa atcaatggat attgccagag
     4441 caaaaggcat caaagtaggg cttcttcgcc cgatcacatt gtggcccttc ccttataaac
     4501 ctttatttga gcttgctggt caggtaaaag gaatgctgac cgttgaaatg aaccttggac
     4561 agatggtcga agacgttcgc ttagctgtta acggtaaagt aagggtggaa cattatggtc
     4621 gcgttggcgg cattatcccg acacccgatg aaatcgtaga agttttggaa actaaaatta
     4681 ttggaggata aaccatggaa acaataacaa gagaacaaat tataaaacct gaaaacctgg
     4741 tttacaaaag aaccaaactg cttaccgaca atgtgatgca ttattgcccc ggctgcggac
     4801 acggaacggc acaccgaatg gtaatggaag tagtggaaga aatgggcatt caggccgaaa
     4861 ctatcggagt agctcctgta ggttgttcgg tatttgcata cgattatatg aacatcgata
     4921 tgcaggaagc tgcgcacgga cgtgcacccg cacttgccac agcaattaaa agactgatgc
     4981 ccgaaaagta tgtttttacc tatcagggcg acggcgacct tgccgccatt ggtactgctg
     5041 aaaccattca tgcatgcaac cgcggtgaaa atatcgtgat catctttata aacaacggta
     5101 tttacgggat gaccggcgga cagatggccc cgacaacatt accgggcatg aaagcatcaa
     5161 cctgtccttt cggaagagat gtcgcaatca tgggaaatcc gttaaaaatg accgaaatag
     5221 tggcaacact tcccggaacc tgcttcgtaa cacggcattc tgtgcatact ccggcaaatg
     5281 tgcgtaaagc caaaagagcc atcagaaaag gatttgagct ttctcatgct ggaaagggcg
     5341 tttccttcat cgaaatcgtt tcaaactgca actcaggatg gaaaatgacc ccggtacagg
     5401 caaataaatg gatggaagaa aacatgttcc cgttctatcc tttgggcgat ataaaagtcc
     5461 ctgctgaata gtttttgaac atacttggat aaatagttat tagtaaaaat aataatataa
     5521 aatattgaaa aatgactgaa gaaattatca tagccggatt tggaggacaa ggtgttttat
     5581 cgatgggtaa aattcttgcc tattcaggcg ttatgcaaaa taaagaagta agctggatgc
     5641 cttcctatgg acccgaaatg cgcggaggta cagccaatgt gacggttatt gtcagtgatg
     5701 aacgtgtcag ttcgccgata cttaccgttt tcgatacagc catcatcctg aaccagcagt
     5761 ccatggataa atttgaaaaa tctgttaaac caggcggatt gctgatatac gacggtaatg
     5821 gcatcacacg ccatcccgaa cgcaaagaca tcaatgtgta ccgagtggat gctgccgaag
     5881 aggcctcaaa actcggaatg aacaaagtgt tcaatatgat ggtgcttggc gcttacctga
     5941 aaattaaacc cattatcagc ccggaattta tcagaaaagg tttggagaaa tctcttccag
     6001 cccgtcatca tggtttgata cccgataacg aaaaagcaat tgagattggg aaagagatca
     6061 tcagggaagt acacaaagta taatcccggc catttatggt cttaaatttt tttattgaaa
     6121 aggcttggta cattttgttt caagcctttt tttattctca aggaaaatat gaaaatacct
     6181 gataagaaaa tcattgaaga agcagcatgc cggataaggc aggccgttgc ccatactcct
     6241 gtgatgacct cacaaagcct ggatgaaatg tttcaggcgc atttgtattt caaatgtgag
     6301 aattttcaga aagcaggggt ctttaaatcc cgcggcgcct gcaatgcagt tttttcattg
     6361 aacgaaaacc aactggacag aggtgtggcg gctcattctt ccggtaatca tgcccaggcg
     6421 ctggcacggg cagctaaatt aagaaataca aaggcctatc tcgtaatgcc tgaaaattca
     6481 tctgaagtaa agatagcggc cgtaaaagcc tatgaaggca tcattacttt ttgcaagccg
     6541 gcacttgaag cacgcgaaac aaccttaaaa gaagtcatat caaaaacggg agcagcagaa
     6601 attcatcctt acgacaatta tcaggtgata gcagggcagg ctactgttac gaaagagctg
     6661 gtcggggata tttccgctct ggattatatc atttgtcctg ttggaggcgg tggattgctt
     6721 agcggtactt tattggctgc aaaatatttt tcgcctcata caaaggtaat agcagccgaa
     6781 ccagaacagg ccaacgacgc gtatttatcg ttcaaatcca aaatatttat tccttcagtg
     6841 aatcccgata caattgctga tgggctaaga acctcgcttg gaacgcttac ttttccgata
     6901 attatggaac atctccatga tattttcaca gtgtcagaaa acgatatcat tgcctccatg
     6961 aaactcatct gggaaaggat gaagatcatt gtggagcctt cttcggcggt aaccctcgcc
     7021 gtattaaaaa acaatcccgg tctctttaaa gaaaaaaaaa cagggatcat catatcaggt
     7081 ggaaatgtgg atattaaaaa ccttccttgg taataatcac tactgatcct ttttatacca
     7141 catataccag gtgagtgagt acatgttggg attgttctgc aattttaatt tttcagattc
     7201 tttcatgctt tttggagcat ggattaaaac atccttgcca atttgccagt tcgcaggagt
     7261 aaggacatta tctttatcgg ccatttgcaa agctttcaac gtacgtaata tctcatcgat
     7321 attcctgcca atattatcag gatatacaaa gaaagccctt accttgttgt caggatcaat
     7381 gaccactact gagcgaacac tttccctgtt ggtggaatcc tgatgcatca tattatattt
     7441 cttcactacc gtcaggtctg catcggcaac caatggaaaa ttgatcttaa tcgcggattt
     7501 tccggtacgg gttatacctt ccatagattt cacccattcg atatggctgt tcaatccatc
     7561 ggtggatata acaatcaaag cagcatttag atatttaaac tcttcctgtt tatcggcaag
     7621 tgcaatgatc tcggaagtgc aaacaggagt aaagtcagca ggatgactga agagtacttt
     7681 ccatttattg tagtagtcag caggaaagtt tatctccccc tgtgttgaaa gagatttaaa
     7741 ctcaggtgca ttcatgccaa gggaaatagg atttttactg acagtctgtg cattagctac
     7801 taaacctgtc agtataagtg ctaatgttaa aaacaatctc ggtttcataa tgtgtttttt
     7861 ttaattgtgt ttatttagac taattaaaga caaagataca acaattaata ccgaaaagca
     7921 agtaaaatgc aaaaaaaatg aaaaatttta cactattttc tgatttcttc ctgatatttt
     7981 tacggtaaat gacagtgata tcattgcttc catgaaactt atctgggaac ggatgaagat
     8041 caatgtgaaa cattcctcgg cggttacaat ggctttaata aagaacaatc ccggtctttt
     8101 taaagaaaaa aggaccggga ttattttgtc aggaggcaat gtggatatga ccaaactgcc
     8161 ctggctgtaa aaaactttta ttttgcagta acaaccgacc ggagatcaat ggtgaaggcc
     8221 agttccattt tcaggatcaa aggatatttc ggattatcaa gtatccagaa tttttttccg
     8281 ctttcggatt tggcaaccat tacttccacg tcctgtttaa cgccgtcaac ttctatagga
     8341 tagttttcgt aacggtcgaa aatgatcgtt tctttgtctt tatcaacagt gacattaatt
     8401 gactgctggc tgatgagttg tttgtatgct ttgcggctga acattgcggt ggtactgttg
     8461 gtcaaattca gagaagaatt gttctcaaaa taatttacca ttttcatagc cgtgtcgatg
     8521 gcagcgtcgt agatggttat gttgccttta tagttgacag gatcagtcat tttccatttg
     8581 aaagccacgt ctaatcccag gaccaggtca cttacaatat aatcatactc tttggtaaca
     8641 ttctgcacgt gataagtcaa cactgaaccg tttttgaacc aggtgctttt aacttcgtgt
     8701 ttttgcgcaa aacttgtgga ggcaattccg gcaaaaatga cgaatgctgc taaaatgaga
     8761 acttttttca taataattag ttttaattga taggcaaaaa taaggaattt tttttattta
     8821 ttcaagtttt ataatttttt aaccaaaaac tgacggattg tcggtatttc caaaagagga
     8881 ggaattgggc tgcctaactc tacttctttg aacgtattga tgtttgtgcg gcaaaaatgt
     8941 ttgtatatta taccgacacg caatacgcat acctgctgaa aggaagttca tattcttgcc
     9001 tgcttcacag catgttgcca tgcttcgtat agttggtcac tttcattttt tgccataacc
     9061 ggttcaaata ctttatcggg tttacgcagt tgggtcagtt gctcttttga ccatagttgt
     9121 acc
//
LOCUS       DAOPYB010000120         9097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9024_length_9097_cov_7.902455, whole genome shotgun sequence.
ACCESSION   DAOPYB010000120 DAOPYB010000000
VERSION     DAOPYB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9097
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9024_length_9097_cov_7.902455"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..844
                     /locus_tag="PKH58_09305"
     CDS             2..844
                     /locus_tag="PKH58_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX89268.1"
                     /translation="MPAYLYEKGNIIRLILFTALFALLFINIFQPFGSRNWYPEVSDF
                     NYFVFSSLIILTGMLVVVISRMFMLYFVRAHSINFWQFSVWILFEILAMSLFYALFTK
                     FIPKEGMNRDFMEIIQKCTINTGLILLLPYSISWLFFSWKEKNRLIEKLQVEETNGVN
                     TKSMIGFSDEKGELKISVMLENLLYIDSADNYATIHYLNKSKLSHYMIRNTLKWMDEN
                     LTKETLLVRCHRSYIVNLDKVKVLRKTKNGIYLEMDAENTPDIPVSKTYYERFMNKFS
                     QYSV"
     gene            1092..2768
                     /locus_tag="PKH58_09310"
     CDS             1092..2768
                     /locus_tag="PKH58_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HNX89269.1"
                     /translation="MEWLNSLLFGEGIAHTILIYSFVIFTGVLLGKIKFWGISLGTTF
                     VLFMGIFIGHLGYTVNHEVLDFLKDFGLILFIYSIGLQVGPGFFSSFKKGGMMLNLLA
                     AGIVLLGVTVAVSFYFILDGRVQMPMIVGIMSGAITNTPGLGAAQEALRQAYDAGQIT
                     EIPQIALGYAVAYPLGVVGIITTIILLRFIFRINLDKEAKELETDDESSMVKPERFTL
                     QLTNQAISGKTLHQVKNMIGRKFIISRMMSDGKYLIPQSETILKQGDILLFISAVSDV
                     EPIMAFIGEPAEMDWKASEKQLVSRRIVITHSKINGKTLGSLRLRSIYGVNITRVNRS
                     GIDLLGTTNLVLQVGDRVMVVGELEDINKVEKVLGNTLKRLNEPPIITIFVGIFIGIL
                     FGSIPFFVPGIPMPVKLGLAGGPLIIAILIGRFGYKLKLITYTTQSANLMLREVGISL
                     FLASVGLASGGKFVETVFSGQGLLWVGIGFVITVLPLLIIGIIGRKFAKLNYFTLIGL
                     LAGSTTDPPALAYSSSISSNDQPAVAYSTVYPLTMFLRVIMAQLIILSFI"
     gene            complement(2847..3428)
                     /locus_tag="PKH58_09315"
     CDS             complement(2847..3428)
                     /locus_tag="PKH58_09315"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNX89270.1"
                     /translation="MNLSNLLNSSIGQTIVKSVADQFGLDESQAASAVNTAVPVILGG
                     LNKNIQTPEGAESLNNALESKHDGSLLDNLGGILSGNVGDLQQDGGSILNHVFGNNVG
                     AVQNGIAQKTGISADKIGPIISMLAPVVMAYLGKQKSQNNVNAGGLGDLLGGMLGGGS
                     AQNGGGLLDMVTGFLDKDKDGSALDDILGMFTK"
     gene            complement(3581..4651)
                     /locus_tag="PKH58_09320"
     CDS             complement(3581..4651)
                     /locus_tag="PKH58_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HNX89271.1"
                     /translation="MKKQILFFLLIISAGTFAQVRNEIQIPDILGYKTLKCDFHMHTV
                     FSDGLVWPTVRVDEAYTQGLDAIAITDHIEYRPHKADLIGASHNRPYEIAEVSAKAKD
                     IILIRGSEITRSMAPGHSNALFLKDCNALDVPAFMDAFKAARAQNAFIFWNHPGWDAQ
                     QPDTTRWFKEHTELYSAGLMQGIEVVNGPEYYPEAQRWCMEKKLTMLGNTDVHEPLTL
                     KEGEHRTMTLVFASERTSEAIREALIDRRTVVYLDDKLIGEEKYLKEIFHQAIEILNT
                     EKTQKYYRITVLNNSDVPFYLHKTSHNPDLIYFRDYTLEPHCKHIITVKLANGVTGGD
                     VNFVITNLLAEPDKGLEINFPI"
     gene            4815..8084
                     /locus_tag="PKH58_09325"
     CDS             4815..8084
                     /locus_tag="PKH58_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX89272.1"
                     /translation="MNKKIILSCIVLLSGLFVTAENPLWMRYPAISPDGTQVAFSYQG
                     DIYKVSVVGGQATRLTTNDAYESNPIWSPDGQKIAFVSDRFNFNKDIFVMPSSGGTAK
                     RLTTHTATEKPYAFTIDGKFVVFSSHYQDPVQSALFPTAMLSELYKVPVEGGRIEMIL
                     ASPTEKISFNKTGSKFLYQDLKGFENTWRKHHTSSVTRDILEYDMKTGSHRKLIDRPG
                     EDTDPLYSPDGNTFCFLSEKSGSYNVFEASISNPQNLKQLTSFSGNPVRFLSIAANGT
                     LCFGYDGEIYTLKKGADKAQKLSVTITNDLENKQEKKMSFSSGATSAVNSPDGKQVAL
                     IVRGEVFVTSTGYNTTKQITKTVAQEADVDFGADNRSLIYSSYRDGYWNIYQAKIARK
                     DDPNFPNATSIEEEVLIKNDKSEKQHPKFSPDGKEVAFVQDRAKLMVYNLENKKLRQI
                     TDGSFQHESNGEMTFDWSPNGKWFTLEYVSNSHAPYSNIGIVSSAGDSEVFDITQSGY
                     TNQKPRWVMDGNAILFNSEQYGMRNHASWGSMEDVMMVFINREAYNKYKMSKEEFELF
                     TDAEKEAKKVEDEAKKKAEEANKDKAENKDKKKTEEKPKDLVVELAGIDERIVRLTPN
                     SSRLGDAIINKEGTKLYYLAAFEEGYDMWVIDLKERSTKLLSKLNGATASISTDKEQK
                     NLFILNGRKMQKMEVSSEKFTSIDFKADMKMDLAQEREAMFNTVYREEKERFYSKNMH
                     GVDWEKLTNSYRKFLPYINNNYDFSEMLSEMLGELNVSHTGSGYRPSSGDDKTAELGL
                     FVSRNDKGLTVDEVLINGPFDTFRSKVKAGDIIEKIDGAEILPDMDYYPMLEGKAGKN
                     TLISVYSPVSGHRWDEVVKPVTAGTLDDLLYNRWIKQRQSDVERLSNGRLGYVHIPSM
                     GDESFRKVYADALGKYYKKEGMIIDIRYNGGGRLHEDIETFFTGKQYLMQEIRGKDYC
                     EMPSRRWNKPSVMMITEADYSNAHGTPWVYKTMKIGKLVGMPVPGTMTSVNWVTLQDP
                     ALYFGIPVVGYRTAEGTYLENSQLEPDVKAPLDLTKAVAGEDTQLEAAVKTLLEELKN
                     N"
     gene            8407..8781
                     /locus_tag="PKH58_09330"
     CDS             8407..8781
                     /locus_tag="PKH58_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNX89273.1"
                     /translation="MEKLTHQEEELMLIIWQNEPGFIKDFLEKMEEPRPPYTTIASVV
                     KNLERKGYVTSRRYGNTYEYKALIREEDYKKKFLSNVVKNYFENSYKDLVSYFAAEKK
                     ISAEELQEIIRLIEKNPQSTKV"
     gene            8913..>9097
                     /locus_tag="PKH58_09335"
     CDS             8913..>9097
                     /locus_tag="PKH58_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX89274.1"
                     /translation="MENLLIYMIKVNGSLILFYLCYKLFFQRDTFWMIRRIYLLVSVL
                     FSFIYPLISVEGWIKKQ"
BASE COUNT         2818 a         1844 c         1809 g         2626 t
ORIGIN      
        1 aataccagca tatctttacg agaaaggcaa tataatccgt ttaattttat ttacggcctt
       61 gtttgccctg cttttcataa atattttcca gccattcggt tcacgcaact ggtatcccga
      121 agtttctgac ttcaattatt ttgttttttc gagtttgatt attctcaccg gcatgctggt
      181 ggtggttatc agccgaatgt ttatgctgta tttcgtccgg gctcacagta tcaatttctg
      241 gcagtttagt gtatggattc tgtttgaaat acttgcaatg tcactgtttt acgctttatt
      301 caccaagttc ataccaaaag agggaatgaa ccgcgatttt atggaaatca tacaaaaatg
      361 caccattaat accggattaa tattgctgct gccctactcc atctcgtggc ttttcttttc
      421 gtggaaagaa aaaaacagac ttattgaaaa gttacaggtg gaagagacta acggggttaa
      481 cacaaaatcg atgattggat tttccgacga aaaaggtgaa ctgaaaattt ctgtcatgct
      541 tgaaaatctt ctttacattg attcggccga caactatgca actattcact acctgaataa
      601 atcaaaattg tcgcattaca tgatacgtaa cactttgaag tggatggatg aaaatctgac
      661 gaaagaaacc ctgctggttc gttgtcaccg ttcgtacatt gtaaatcttg acaaggtgaa
      721 agtgcttcgc aaaacaaaaa acggtattta cctcgaaatg gatgccgaga acacacccga
      781 tatacctgtt tcaaagactt actacgagcg attcatgaac aaattctccc aatactctgt
      841 ctgaaatctc aacggagcga aaactgtcct gtattaaatg atacgcaaat attagcgtta
      901 aatttctaaa agcttctttc cgtctccccc ctccgactta aaacaactga atttctgcat
      961 gttaaccttt tttgaaatat tattctcagg atatactaac tttttgaata aaattaacta
     1021 attttgcacc cacaaatcgg gaaacaggaa ttttatttta agactctata attcaattaa
     1081 ttatatattc aatggaatgg ttaaattctt tattatttgg tgaaggtatt gctcacacca
     1141 ttttgattta ctcttttgta atcttcacgg gagtattgtt aggaaaaatc aaattttggg
     1201 gaatctctct gggtactacc tttgttcttt ttatgggtat ttttattgga caccttggtt
     1261 atactgtcaa ccacgaagtg cttgattttc tgaaggattt cggtttaata ctctttattt
     1321 actcaattgg tttacaggtt ggtccgggtt ttttctcatc cttcaagaaa ggtggaatga
     1381 tgctcaacct ccttgctgcc ggaattgtac ttttgggagt tactgttgct gtatcgttct
     1441 atttcatact cgatggccgg gtacaaatgc cgatgattgt gggcatcatg tcgggagcta
     1501 tcaccaatac tccgggactt ggtgctgctc aggaagcatt gcgtcaggct tatgatgccg
     1561 gacaaatcac tgaaatcccg caaattgcgt tgggctatgc cgtagcatat ccactgggtg
     1621 ttgtcggtat tattaccact attatacttc tccggtttat tttccgaatc aatctggata
     1681 aagaagccaa agaacttgaa accgacgatg aatcgtcaat ggtgaaaccg gagcgcttca
     1741 cgttgcaact aaccaatcag gctatcagcg gaaaaactct gcatcaggtt aaaaacatga
     1801 ttggccgtaa atttatcatc tcacgcatga tgagcgatgg aaaatattta attcctcaat
     1861 ctgaaacaat tctgaaacaa ggagatattc ttttattcat atctgcggtg tctgatgtgg
     1921 aacccatcat ggcatttatt ggcgaaccag cagaaatgga ttggaaagct tccgagaaac
     1981 aactggtatc gcgtcgtatt gttattaccc acagtaaaat taacggtaaa acacttggat
     2041 cattgcgttt acgttctatt tacggtgtaa atataacccg tgttaaccgt tcgggtattg
     2101 acctgctcgg cacaacaaac ctggtactac aggtgggtga ccgagtgatg gtggttggtg
     2161 aactggaaga tataaataag gtagagaaag tacttggtaa cacactgaaa agactgaatg
     2221 aaccccctat tatcaccatc tttgtgggaa ttttcattgg cattcttttt ggaagcatac
     2281 cattctttgt tcccggaata ccgatgccgg tgaaacttgg tcttgcaggc ggtccgttga
     2341 ttattgcaat ccttatcggc cgcttcgggt ataagctgaa acttataact tacaccacac
     2401 agagtgccaa cctaatgctt cgcgaagtgg gtatttcgct attccttgcg agcgtcggac
     2461 ttgcatcagg tggaaaattt gtagaaaccg tattttcagg tcaaggattg ctatgggtag
     2521 gtatcggttt tgttataacc gtgttaccat tattgattat cggcattatc ggacgcaaat
     2581 ttgcaaaact caactatttt acgctgatag gtttgctggc aggaagtact accgacccac
     2641 ccgcgctggc ttattcaagt tctatttcaa gcaacgacca gcctgcagta gcatactcta
     2701 ctgtttatcc acttactatg ttcctccgtg tgataatggc tcagttaata attctttcat
     2761 tcatatagta agtatctaaa ttattccata aaaaaaggag tttaccagat ttggcaaact
     2821 ccttttttat gaaaataaca attttattat ttagtaaaca tgcccaggat atcatcgaga
     2881 gcactaccgt ccttgtcctt atcgaggaag cctgtaacca tatcgagcag gccaccacca
     2941 ttctgagctg aaccacctcc aagcatacct cccagcaaat cacccagacc accggcattt
     3001 acattgttct gacttttttg tttaccaaga tatgccatca ctaccggagc aagcatagaa
     3061 ataataggac caattttgtc agcgctgata ccagtttttt gagctatacc gttttgaacg
     3121 gcgcccacat tattgccaaa cacatggttc aaaatacttc caccgtcttg ctgcaggtca
     3181 cctacatttc ccgaaagtat gccacccaga ttatccagca gacttccatc gtgtttcgat
     3241 tccagcgcat tattcagact ttctgcaccc tcgggagttt ggatattttt gttcaatccg
     3301 cctaatataa caggtactgc cgtattcaca gcagaagcag cctgactttc atccagacca
     3361 aactgatctg caacactttt cacaattgtt tgcccaattg aactgttcag taaattagat
     3421 aaattcataa gattaatttt tattgattta taaatagaga tgaattgata atcgtactat
     3481 gtatagcgtt tatagaaaaa aaagttcccg gaacagtgtt atatttatta aattatttct
     3541 aaaatgtcaa ttttgtttat ttatcttcag acgaataagg tcagatagga aaattaattt
     3601 ccagcccctt atcaggttcg gctaataaat ttgtaataac aaagttcaca tcgcctccgg
     3661 taactccgtt tgccagcttt accgtaataa tgtgtttaca gtgaggttcc agggtataat
     3721 ccctgaaata aattaaatcg ggattgtgcg aggttttgtg caaataaaag ggtacatcag
     3781 aattattaag gactgttatc cggtagtatt tttgtgtctt ttcagtattc agtatttcta
     3841 tagcctgatg aaaaatctct ttcaggtatt tctcttcgcc aataagttta tcgtccaggt
     3901 aaaccacagt cctacggtca atcaaagctt cccggattgc ttcagaagta cgttcgctcg
     3961 caaaaacaag ggtcatagtg cggtgttcac cttctttcag tgtgagaggt tcgtgaacat
     4021 cggtgttgcc gagcatggtc agttttttct ccatacacca tcgttgagct tcggggtagt
     4081 actcaggtcc gttgaccact tcaatcccct gcataagacc ggcactgtaa agttcggtat
     4141 gttccttaaa ccaacgtgta gtatctggtt gctgtgcatc ccacccggga tgattccaga
     4201 agataaaagc attctgagct ctggcagctt tgaaagcatc cataaaagca ggcacgtcca
     4261 gcgcgttgca atctttcaga aaaagtgcat tcgaatgacc cggagccatg gagcgggtaa
     4321 tttccgaacc acggataagt atgatatctt tcgctttggc tgaaacttcg gctatttcat
     4381 acggacgatt gtgtgatgcc ccgataagat cagccttgtg cggtcggtat tctatgtgat
     4441 cggtaatggc aatggcatca agcccctgag tataagcttc gtcaactcga acagtaggcc
     4501 acacaagtcc gtcggaaaag accgtatgca tgtgaaaatc gcatttaagc gttttatacc
     4561 ccaatatatc agggatttga atttcatttc tgacctgagc aaaagtaccc gctgaaatta
     4621 ttagcaaaaa aaataaaatt tgttttttca ttgtataact gttttttcaa aaatcatatt
     4681 tcacgaaatt acacattttt tcatcaaaaa ggaacaaata ctttgttttt tattattcta
     4741 acaaaataac attttcatat attaggaaaa tattaatact tttgtacact tttaaaaaac
     4801 ataaataaat aatcatgaat aaaaaaatca ttttatcttg tattgtttta ttatccggct
     4861 tatttgttac cgctgaaaat cctttgtgga tgaggtatcc ggctatttca ccagatggca
     4921 ctcaggttgc attttcgtat cagggtgata tttataaagt gtcggtagtt ggcggacaag
     4981 ccacgcgact taccaccaac gatgcctacg aaagtaaccc gatttggtcg cccgacggac
     5041 aaaaaatagc ttttgtaagt gatcgcttca acttcaacaa agatattttt gtgatgcctt
     5101 caagtggtgg taccgccaaa agactgacaa cacacaccgc aacagaaaaa ccatacgctt
     5161 ttaccatcga tggcaaattt gttgtttttt catcacatta tcaggatcct gttcaaagcg
     5221 cattatttcc tactgccatg ttgtccgaac tttacaaagt accggttgaa ggcggacgaa
     5281 ttgaaatgat tctcgcttca ccgactgaaa aaatcagttt caacaaaacg ggcagtaaat
     5341 tcctgtatca ggatctgaaa ggatttgaaa atacctggcg caaacaccac acttcctccg
     5401 tgacgcgaga tattctcgaa tacgacatga agaccggctc tcaccgaaaa ctgattgatc
     5461 gtccgggcga agataccgac cctctttaca gtcccgacgg caatacattc tgcttcctta
     5521 gcgaaaaaag cggcagttac aacgtattcg aagcgtcaat cagtaatcct caaaatttga
     5581 aacagctgac ctcattcagt ggtaacccgg tacgttttct aagtattgca gctaatggta
     5641 ccctctgttt tggttatgac ggtgaaattt acaccctgaa aaaaggtgct gataaagctc
     5701 aaaaactatc cgtcaccatc accaatgatt tggagaataa acaggaaaag aaaatgagtt
     5761 ttagctcggg agctacatcg gctgtaaatt caccggacgg caaacaggtt gcactgatag
     5821 ttcgcggtga agtttttgtt acttctaccg gatacaacac aactaaacaa ataaccaaga
     5881 cggttgcaca ggaagctgat gtggatttcg gagccgataa ccgttcgctg atttactcca
     5941 gctaccgcga tggatactgg aatatctatc aggctaaaat agcacgcaaa gacgatccga
     6001 attttccaaa tgcaaccagt atcgaagaag aagttcttat taaaaacgat aaatccgaaa
     6061 aacaacatcc taagttctca cccgacggaa aagaagtggc ttttgtacag gacagagcta
     6121 aactgatggt ttacaacctt gaaaacaaaa agctccgtca gatcactgac ggctccttcc
     6181 agcacgaaag caatggtgag atgactttcg attggtcgcc aaacggcaaa tggtttactc
     6241 tggaatatgt atccaacagc cacgccccct actccaatat agggatcgta agttcagccg
     6301 gtgacagtga agtattcgac attacgcaaa gcggctatac caaccaaaag ccccgttggg
     6361 taatggatgg taatgctatt cttttcaatt ccgaacaata cggtatgcgc aatcatgctt
     6421 cgtggggttc tatggaagac gtgatgatgg tatttattaa ccgtgaggct tacaacaaat
     6481 acaaaatgtc taaggaagaa ttcgaattat ttaccgacgc cgaaaaagaa gcaaaaaaag
     6541 tagaagatga ggctaaaaag aaagctgaag aagccaacaa agacaaggca gaaaataagg
     6601 ataagaaaaa aacagaagaa aaaccaaaag accttgtggt ggaactggcc ggaattgatg
     6661 aacgaattgt acgcctcaca ccgaattctt ccagacttgg cgatgcaata atcaacaaag
     6721 aaggcaccaa actgtattat ctggctgctt tcgaggaagg atatgacatg tgggtaattg
     6781 acctcaagga acgttcaacc aaactgttga gtaaactgaa tggtgcaaca gccagtatct
     6841 caacagataa agaacaaaaa aaccttttta tactgaacgg cagaaaaatg cagaaaatgg
     6901 aagtctcatc cgagaaattc acctccattg atttcaaagc cgacatgaaa atggatctgg
     6961 cacaggaacg tgaagctatg tttaacaccg tgtaccgcga agaaaaggaa cgtttctatt
     7021 caaaaaatat gcacggagtt gactgggaaa aactgaccaa tagttaccgg aaattcctgc
     7081 catacataaa caacaactac gatttctccg aaatgctcag cgaaatgctg ggcgaactga
     7141 atgtttcaca taccggctcg ggctatcgtc cttcttcggg cgacgacaaa actgctgaac
     7201 tcggattgtt tgtaagcaga aatgataaag gattaactgt tgatgaggta ctcatcaacg
     7261 gaccattcga cacattccgc tcaaaagtga aagccggcga tatcattgaa aaaattgacg
     7321 gcgcagaaat attaccagat atggactatt atccaatgct ggaaggcaaa gccggtaaaa
     7381 acaccctgat ctcggtatat tcacccgtat cgggacatcg atgggatgaa gttgtaaaac
     7441 ccgttacagc cggcactctt gatgacctgc tctacaaccg ctggataaaa caacgccaat
     7501 ccgatgtgga acgtttgtca aacggtcggt tgggttacgt gcatatacct tcgatgggcg
     7561 acgaaagttt ccgaaaagta tatgctgatg cactcggtaa gtattacaaa aaagaaggaa
     7621 tgattataga tatccgctac aacggtggtg gacgtcttca tgaagatatc gaaacttttt
     7681 ttaccggaaa acaatatctg atgcaggaaa tacgtggtaa agactactgc gaaatgccca
     7741 gccggcgctg gaacaaacct tcggttatga tgataaccga agccgattat tccaacgcgc
     7801 acggaacgcc gtgggtgtat aaaaccatga aaataggtaa actcgtaggt atgccagtgc
     7861 ccggaaccat gaccagtgtc aactgggtta ccctgcagga tcctgcgttg tatttcggca
     7921 ttccggtagt aggttaccgt actgcagaag gcacatacct cgaaaacagc cagctggaac
     7981 ccgacgtaaa agccccgctc gacctcacca aagctgttgc aggtgaagat acgcagctgg
     8041 aagctgcagt aaaaacgttg ctggaagaat taaaaaataa ctaatttcta catccggcag
     8101 aataattcaa ataaaaattt aatgcatttt atcctgaaac aggttatttt tcaataatcc
     8161 tgtttcagga tttttttatg atcggcaacc agcagcatta ccctttcatt agtctaattt
     8221 cagacagtta aacaaataaa caggatgtaa caacatgaag accggttatt cttacgaggt
     8281 taacaacgtt aaatgtagtt aattataaga aaagtatttc ttttactacg aacttttcgt
     8341 agctttgcga tgttttcgta atagattgat attttggata tttatcagtt cgtaaaaaat
     8401 atatttatgg aaaaacttac acatcaggaa gaagagctta tgttgatcat ctggcaaaat
     8461 gaaccgggtt tcataaaaga ttttctggaa aaaatggaag aacctcgtcc gccatacacc
     8521 accattgcct cggttgtaaa aaatctggaa cgcaaaggct atgtcacctc cagacgatat
     8581 ggaaacacgt atgaatacaa ggctttaatc agagaagagg attacaaaaa gaagtttttg
     8641 tcgaacgttg tgaaaaatta ttttgagaat tcttacaagg atttagttag ttactttgcc
     8701 gctgaaaaaa agatttcggc ggaagaactt caggaaataa tcagattgat tgaaaaaaat
     8761 cctcagtcaa ccaaggtcta agatttgaga attcaatatt atttactgta ttggaattcc
     8821 ggtttttatt atgactttaa aaatttacat tactctgtaa atcctcctaa acagaacatt
     8881 ctgtcatttg ggacaaaaaa aggacatcgg ctatggaaaa tttgttaatc tatatgatca
     8941 aggtaaacgg atcgttaatc ctgttttacc tgtgttacaa attgtttttt caacgggata
     9001 cattttggat gatacgccgg atatatctgc tggttagtgt gttgttctca tttatctatc
     9061 cgctcatttc tgtggaagga tggataaaaa aacagga
//
LOCUS       DAOPYB010000121         9093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9030_length_9093_cov_9.519363, whole genome shotgun sequence.
ACCESSION   DAOPYB010000121 DAOPYB010000000
VERSION     DAOPYB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9030_length_9093_cov_9.519363"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..783
                     /locus_tag="PKH58_09340"
     CDS             151..783
                     /locus_tag="PKH58_09340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX89275.1"
                     /translation="MLTPKITKEEVNALPIAEFRGEIIIVDEPVKIKPALEYLKQQKI
                     VGLDTETKPSFQRGTIHKVSLIQISTLDKCLLFRLNKIGFPEELLRFLADEKIKKVGL
                     SLRDDFNGLKKRSAIKPANFIDLQNIAKDYGILELSLQKIYAILFNRKISKSQRLSNW
                     ENPVLTEQQACYAATDAWACLQIYLHLIEKEKLTQKEIEKLISEVNSIQQ"
     gene            812..1990
                     /locus_tag="PKH58_09345"
     CDS             812..1990
                     /locus_tag="PKH58_09345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNX89276.1"
                     /translation="MITVQLKPKKEESLLRFHPWVFSGAIQRITGKPQEGEVVEVVDH
                     QGNFLAVGHYQIGSIAVRVMSFDKQNIDFAFWQKKISQAYGVRVELKLINPPYNNTYR
                     LIHGEGDFLPGLIVDVYDDTAVFQAHSVGMHLNRQMIAEAIVAAVPAVRNVFYKSETT
                     LPFNAPVNPEDGYLIGKASDTFKAVENGLELQVDWLKGQKTGFFVDQRDNRALLEKYS
                     AGKKVLNMFCYTGGFSVYALRGGAKLVHSVDSSAKAIELTDKNVSINFPDTKDHCSYA
                     EDAFKFLNDMHEEYDLIILDPPAFAKHRDAVRNALKGYKRLNLKALQQIKPNSILFTF
                     SCSQVISKDQFRLAVFSAAAEAKRNVRILHQLTQPADHPINIYHPEGEYLKGLVLYVE
                     "
     gene            2201..2794
                     /locus_tag="PKH58_09350"
     CDS             2201..2794
                     /locus_tag="PKH58_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX89277.1"
                     /translation="MKNIFQLTDDILVRLYEEGNNEAFEVLLMRYKSKVYTYIYLIVR
                     NKELTEDIFQETFIKAISTIQQGRYTETGRFLGWINRIAHNLIIDHFRREKNENTFSA
                     DGIEYDIINNTKLSEKSVEDTINNDQVLTDVVNLINYLPQTQQEVIRMRFFEDLSFKE
                     IAERTEVSINTALGRMRYALLNMRRIAAENDIQLELK"
     gene            2855..3037
                     /locus_tag="PKH58_09355"
     CDS             2855..3037
                     /locus_tag="PKH58_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89278.1"
                     /translation="MPMRKVSTLINSTSRINSQYDFIPSEMSPSETTVNKILQFAAAY
                     KVEKVTENDIIGFILN"
     gene            3916..4563
                     /gene="rpe"
                     /locus_tag="PKH58_09360"
     CDS             3916..4563
                     /gene="rpe"
                     /locus_tag="PKH58_09360"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNX89279.1"
                     /translation="MSKFISPSLLAADFGNLQSACEMINASEAQWLHIDVMDGVFVPN
                     ISFGFPVLEAVKKHCNKPLDVHLMIVEPEKYIERFIKAGADILTVHLETLNNPISTLR
                     QIKEMGVKAGLTINPDIPVQSLRSLVSEADMVLLMSVFAGYGGQKFIEETYARIEELK
                     DIIADENPDCLIEIDGGVNTENAPLLFAAGVDVLVAGNSVFHSPDPIETIHKLLN"
     gene            4585..>6577
                     /locus_tag="PKH58_09365"
     CDS             4585..>6577
                     /locus_tag="PKH58_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNX89280.1"
                     /translation="MNFLHRTPFFRLLLAIIPGIIFYQYINVPAYLLYLLVALSAITI
                     LIAFLFRRSRFQYKFRWIFGAGTGVFLSVLSYFLCLQFDKNHPFSALNEHAVYEVSLT
                     ASPVEKARSYLCKAELIRNLDSTQVKAASGHAIIYLEKDSAVRHLLLGDRLLIDAEFK
                     SPDGVQNPDGFDYARYLKRQGILATAYVPTEKWQKSPATPRFSPAEPFKFLLRQADKC
                     RIFLLDIYRKYNITGDEFGVLAALTLGYTDELQPDLLKSYSATGAMHILSVSGLHVGI
                     VYAVILFLLGFLSKNKRQKILKIILIMLFLWTYAFITGLSPAVLRATIMFSFVTVSAG
                     MGWKSQIYNNIFASAFCLLLYNPNYLFDIGFQLSYSAVLSIVFFQPQISGWIFISNKL
                     LKKVWDLVAVSLAAQLGTAPFVLYYFHQFPNFFLLTNLVAIPLSTVIIYLAIGLLLIT
                     FIPFLSSAVAFLLKWSLWILNFLITGIQNLPFSISTVSMDFRQLLFAFFGIAFLSAYF
                     YRKNFNVLIAGLISILLVCGMFTFQKYKSLTTTKFIVFADSRVPIINFVERGRNYVYT
                     TDRIQAEKIAGSFWKTNLLKSPEYIESTSWFNDGFAGFHEKRFFILKDTLLKNKTTSQ
                     PLQVDYLILSNKIKPNIEQILKCVHPRTVVADKSISKWYT"
     assembly_gap    6578..6677
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6678..7679
                     /locus_tag="PKH58_09370"
     CDS             <6678..7679
                     /locus_tag="PKH58_09370"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNX89281.1"
                     /translation="EELVHKTKIEIENAENIVIVTHVGPDGDAMGASLGLWHFLMTIE
                     KVPQVIVPSPFPNFLAWMPGADKTLTYSRDKEKADECIQNADLIFVLDFNATNRMDKV
                     ADAVVASKAKKVMIDHHLEPEHFTNIIISYPEISSTSELVFRLICRMGQFSDINLACA
                     ECIYTGMMTDTGGFTYNSNHEEIYSIIYELIRLGVDKDDIYRKVYNTFSADRMRLMGY
                     CLYKKMKIYPEYQAALITLTKKELQEFNYDNGDAEGFVNIPLSIKGINFTVFMREDPA
                     KIKVSLRSQGTFPANKFAAEVFGGGGHLNASGGESYTTLDEAVRKFEEALPLYTEFL"
     gene            7875..>9093
                     /locus_tag="PKH58_09375"
     CDS             7875..>9093
                     /locus_tag="PKH58_09375"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HNX89282.1"
                     /translation="MKYIIILGDGMADEPVSSIGNQTPLQFAKKPYIDKLASMGRCGM
                     LDTIPQGYAPGSEIANLSVLGYDLNECFEGRGTLEAASIGVEIGENEMAMRCNLICIE
                     DGKIKNHSAGHISTEEADVLIRFLQEKLGDDRVSFHTGVSYRHLLKLKNGNKNISCTP
                     PHDVPGTEFRDVMIQPLASEAKETADYLNELTIKSQQLLENHPINLKRAELGKDKANS
                     IWVWSPGYKPQMKKMTEIYPVNSGSVISAVDLIKGIGIYAGLKSINVEGATGLYTTNY
                     EGKVQAAIEALKTDDFVYLHIEASDEAGHEGDVELKIRTIEYLDSRIVKPIMEELLTW
                     NEPVTIALLPDHPTPCAIRTHTATPVPFVIYRTGNTPDEVARYDEFTCQSGSFGLLKG
                     NEFINEVFRNQPGS"
BASE COUNT         2727 a         1864 c         1831 g         2571 t
ORIGIN      
        1 ctgctgaatg ctttgcgcgc tcttcactct ctgcagtaca gcgatacttt cggaaatgat
       61 gaagacaaca ctgatttcaa tcctgatgat tacaaaaagc ttgatcggga cagttttctg
      121 agatttcaaa cagaagatta aacacaaaaa atgcttacac ccaaaataac aaaagaagaa
      181 gtcaacgcgt tacccatagc cgaattcagg ggagagatta ttatcgtcga tgaacctgtt
      241 aaaataaaac cggcgctgga atatctgaaa caacaaaaga ttgttggtct ggatacagag
      301 acgaaacctt catttcaacg cggaaccata cacaaagttt cattaataca gatttccacc
      361 ctggacaaat gcttactttt cagattgaat aaaattggct ttccggaaga attactgcga
      421 tttctggccg atgaaaagat aaaaaaggtc ggactttctt tacgtgatga tttcaacggg
      481 ctgaaaaaaa gatcggccat aaagcctgct aattttattg acttacagaa tatagccaaa
      541 gattacggaa tacttgaact tagtctgcag aaaatatatg ctatcctttt caatagaaaa
      601 atatcaaaat cgcaacgtct gagtaactgg gaaaacccgg tacttaccga gcaacaggca
      661 tgctacgctg ctaccgatgc atgggcgtgc cttcagattt atctgcacct gatagaaaaa
      721 gaaaaactca cgcagaaaga aatcgaaaaa ctgatttcgg aagtgaattc cattcaacag
      781 taaacaaaca aatttacagc atttattagc catgataaca gttcagttaa aaccaaaaaa
      841 ggaagaaagt ttactacgtt tccacccgtg ggtcttttca ggcgctattc agcgtattac
      901 gggaaagcct caggaaggag aagtggtaga agttgttgat catcagggta acttcctggc
      961 tgtcggacat taccagattg gcagtattgc tgtacgtgtt atgagtttcg ataagcaaaa
     1021 tatcgatttt gccttttggc aaaaaaaaat cagtcaggct tacggagttc gtgttgaact
     1081 taaacttatc aatccgcctt acaacaatac ttacaggctt attcacggcg aaggtgattt
     1141 tctccccgga ctgattgtcg atgtatatga cgatactgct gtatttcaag cacattcggt
     1201 aggaatgcac ctaaatcgcc agatgattgc agaagcaatc gttgctgctg ttccggctgt
     1261 cagaaatgtg ttttacaaat cagagaccac cctacccttc aatgctcccg ttaatccgga
     1321 agacggatat ctgatcggga aagcaagtga tactttcaaa gctgtggaaa acggattaga
     1381 acttcaggtg gactggctga aaggacaaaa aaccggcttc tttgttgacc agcgtgacaa
     1441 ccgggcattg cttgagaaat actcggcagg gaaaaaagtg ctgaatatgt tttgttatac
     1501 cggtggtttt tcggtgtatg cactgcgggg tggtgcaaag cttgtacatt cagtagatag
     1561 ctcggctaaa gccattgaac ttacagacaa aaatgtatcc atcaattttc cggacacaaa
     1621 agatcattgc tcctatgctg aagatgcatt caaatttttg aatgatatgc acgaagaata
     1681 cgacctgata atacttgatc ctccagcgtt tgccaagcac agggacgctg tacgcaacgc
     1741 tctgaaagga tacaaacgac tgaatttgaa agcattgcaa caaataaaac caaacagcat
     1801 actatttact ttctcctgtt cgcaggttat ttccaaagat cagttccggc tcgcggtttt
     1861 cagcgcggca gccgaagcca aacgtaacgt gcgtatcctt catcagctta cacagcctgc
     1921 cgatcatcct attaatatct atcaccctga aggagaatat ttgaaaggat tagtacttta
     1981 cgttgaataa tttcggttaa catcttgagg ctttatatat ggctaacctt tttatttaat
     2041 catttttagt atttatttgc acattaactg catttatttt tggaattgaa aaaagtaata
     2101 tttacatttg caacctaaat aataatatca acaagtttta accaactaat ttcaatacgc
     2161 ctattgctca gaattactct atttttaaaa tctagaagta atgaaaaaca tatttcagct
     2221 aactgacgat attttggtca ggttgtatga agagggcaat aacgaggcat ttgaggtatt
     2281 attaatgcgc tacaaatcta aggtttatac ctacatttat ttgattgtac gcaacaaaga
     2341 actcaccgaa gatatattcc aggaaacgtt tattaaagcc attagtacca tccagcaggg
     2401 tcgttataca gagaccggca ggttcttggg atggattaac cgcattgctc acaatctgat
     2461 tattgatcat ttccgccgcg aaaaaaatga aaacacattt tcagcagacg gaattgaata
     2521 cgacattatc aacaatacaa aattatcgga aaaaagtgtg gaagatacca tcaataacga
     2581 tcaggtatta actgacgtgg ttaacctgat taattatctt ccgcaaacgc aacaggaagt
     2641 aatccggatg cgtttttttg aagatttaag tttcaaggaa atagcggaac gtacagaagt
     2701 cagcatcaat acagctttag gccggatgcg atatgcacta ttaaacatgc gccgcattgc
     2761 tgctgaaaat gacatacaat tagaactgaa ataatttttt gaggtgatat actataacgc
     2821 ataagcatcc gttttataaa taaaaaacaa gcaaatgcct atgcgtaaag tttctactct
     2881 gattaactca acatccagaa ttaactcaca gtatgatttc atcccctcag aaatgagccc
     2941 aagtgaaaca acggttaata aaatcctgca gtttgcggca gcttataaag ttgagaaagt
     3001 tactgaaaat gacattatcg gatttatttt gaattaaatg aaagaggtta gattgactaa
     3061 cctctttttt tattatcaca tttattgttg tccggtaaac attttagaaa aatcctgtaa
     3121 gtccttataa aacgtgattt gccataatgt cgcctctata aggctttata ttctgttaca
     3181 tattatttct accataattt tgcctctacg aggctttaat ttttagcacc agcggtgcga
     3241 cattattgta gaaaacagat tacacccatc atcaaaagct cctataggag cgacattatt
     3301 ttttttgttt ttatcggaca ataataaatc acattataat taacgtgagt tcgaaaaagg
     3361 atatgcatta ttaataacct ctagtgcgta atcatatatt tcattacatg acaaaaattc
     3421 tattcgatca tataaacttg tatttcagtt ccataaccca ggggcttccg ccccaaaccc
     3481 cgacttactt tttgtcatga cacaaaaagt aagcaataaa gtcaatccgc cagctggcgg
     3541 acgcttcaca cgaaaaactt gcgttcggtc ggctaaaacc gtccaaactc gcgatggcgg
     3601 aatacgccgc cactcaaaca ggacagtttt ttaacgccac cctcacttgt ttttcggctc
     3661 accggacgag gtcgctcgtt agcgagcctt tataacatac ataagattaa ataattatat
     3721 gatttgactt ttttgtcatg tactaatacc atatatttca aacgcacgtt aattcagtaa
     3781 atacttcatc gagaagaatg agaggcattg cataaaatca ctgcttctct ttttttttga
     3841 aaattaatta ttttcggatt tattgaaaat cttctttttt tctgtaagtt tgtaaattaa
     3901 aatacgtaca gtgttatgag taaatttatc tcgccctcgt tgcttgctgc tgattttggc
     3961 aatctgcaat cagcctgcga aatgattaat gccagtgaag cgcaatggct gcacatcgat
     4021 gttatggacg gagtttttgt gccgaatatt tcatttggtt ttccggtgct ggaagctgta
     4081 aagaaacatt gcaataagcc gctggatgtt catttgatga tcgtggaacc ggaaaaatat
     4141 attgaacgtt tcattaaggc gggtgccgat atactcactg tccatctcga aacactgaat
     4201 aatccgattt ccaccctgcg tcaaataaaa gaaatggggg tgaaagcagg tctgacaatt
     4261 aatcccgata ttccggtaca aagtctgcga agtctggtaa gtgaagccga tatggtactg
     4321 ctgatgtcgg tttttgccgg ttatggcgga cagaagttta tcgaagagac ttatgccaga
     4381 atagaagaac tgaaagacat cattgctgac gaaaatcccg attgcctgat tgaaattgat
     4441 ggcggcgtga ataccgaaaa tgctccgtta ctttttgctg ccggtgtaga tgttttggta
     4501 gcaggtaatt ctgttttcca ttcgcccgac cctattgaaa ctatccataa gttacttaac
     4561 taaaaaagtg cagatggaag atatatgaat tttctgcaca ggacaccctt cttccgctta
     4621 ttgcttgcaa taattccggg tatcatattt tatcagtata tcaatgtacc tgcgtatctg
     4681 ttgtatcttc ttgttgcgtt atcagcgatt actattctta ttgcttttct gtttcggaga
     4741 tctcgttttc agtataaatt caggtggata ttcggagcag gtacaggagt ttttctttcg
     4801 gtactgagct attttctgtg tcttcagttt gataaaaatc atccgttttc agccctgaac
     4861 gaacacgccg tttacgaagt gagtttgacc gcgtcgccgg ttgagaaagc cagatcttac
     4921 ctttgcaaag cagaacttat ccgaaatctt gactccaccc aggtaaaggc ggccagcgga
     4981 catgctatta tttatctcga aaaagacagc gctgtcaggc atttgctgct cggcgacagg
     5041 ctgctcatcg atgctgaatt taaaagcccg gatggagtac agaaccctga cggttttgac
     5101 tatgcacgtt atctgaaacg acaaggcata ctggctacag cttatgttcc cacagagaaa
     5161 tggcaaaaat cacctgctac accacgtttc tcaccggctg aaccttttaa gtttcttttg
     5221 cggcaggcag ataaatgccg gattttttta ctggatattt accggaaata caatatcacc
     5281 ggtgacgaat ttggtgttct ggccgcactg acactgggtt ataccgacga actacaacct
     5341 gatctgctca aaagttacag tgctaccggc gccatgcata tcctttcggt aagcggactt
     5401 catgtgggaa ttgtctatgc tgtcattctg ttcttactgg gttttctgag caaaaacaaa
     5461 cgtcagaaaa ttctgaaaat tatcctcatc atgctgtttt tatggacgta cgcttttatt
     5521 accggccttt caccggcagt attaagggcc accattatgt tcagttttgt taccgtatcg
     5581 gcaggaatgg gatggaaatc gcagatatac aacaatattt tcgcctcggc tttttgcctg
     5641 cttctctaca acccgaacta cctcttcgat attggttttc agttaagtta ttccgccgta
     5701 ttgagcatcg tattttttca gccgcagatt tccggctgga tatttatctc caataaattg
     5761 ctgaaaaaag tctgggacct ggtagccgtt tcgctggcag ctcaactggg cactgccccg
     5821 tttgtacttt attatttcca tcagtttcca aatttctttc tgctcaccaa cctggttgcc
     5881 attccgcttt cgacagtaat catatatctt gccataggac tgctattgat aacatttata
     5941 cccttcctgt catcagctgt cgcgttcctt ttaaaatggt cactttggat acttaatttt
     6001 ctgattacag gtatacaaaa tctgcctttt tccatcagca ccgtatcgat ggatttccgg
     6061 caactgctgt ttgctttttt tggtatcgcg tttttatccg cttatttcta caggaagaac
     6121 ttcaacgtat taattgccgg attaatctcg atactgctcg tgtgcggaat gttcaccttt
     6181 cagaaataca aaagtctgac cacaacaaag tttattgttt ttgcggattc gagagtgccc
     6241 atcataaact tcgtggaaag aggcaggaat tacgtttaca ccacagacag aatacaagct
     6301 gaaaaaatag ccggttcgtt ctggaaaacc aatctgttga aatccccgga atatatcgaa
     6361 agtacttcgt ggttcaacga tggttttgcc ggttttcatg agaaacgttt tttcatactc
     6421 aaagacacgc tgctgaagaa taaaaccacc tctcaaccac ttcaggtcga ttatctgata
     6481 ctgtccaata aaattaagcc caacatagag cagatactta aatgtgttca tccccgtacg
     6541 gttgtagcag ataaaagcat ttcgaaatgg tacaccgnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnngaa gagctggttc acaagactaa aattgaaatt gaaaacgccg
     6721 aaaatattgt aattgttaca catgtaggtc ctgatggcga tgcaatgggc gcttcgctgg
     6781 ggttgtggca ttttctgatg accatcgaaa aagtaccaca ggtcattgtg ccttcgccat
     6841 ttcctaattt cctggcatgg atgcccggag ccgataaaac gctgacttac agccgcgaca
     6901 aggaaaaagc ggatgaatgc attcagaatg ctgatctgat atttgtactt gatttcaatg
     6961 ctaccaaccg gatggataag gtagctgacg ctgtcgttgc ttccaaagca aagaaagtga
     7021 tgatcgacca tcacctggaa cccgaacatt ttacaaacat tatcatatcc tatcccgaaa
     7081 tctcgtccac cagcgaactg gtattcagac ttatatgccg gatgggacaa ttctcggata
     7141 tcaacctggc ctgtgccgaa tgtatttaca ccggcatgat gaccgatacc ggcggattta
     7201 catacaattc caatcacgag gaaatttatt ccatcatcta cgaactgatc agactggggg
     7261 tggataagga cgatatatac cggaaggtgt acaatacctt ttcggccgat agaatgcgac
     7321 taatggggta ttgcctgtac aagaaaatga aaatatatcc cgaatatcag gcagcactca
     7381 ttacactgac caaaaaagaa cttcaggaat ttaattacga taatggtgac gctgagggtt
     7441 ttgtaaatat tccgttatct atcaagggaa ttaacttcac cgttttcatg cgtgaagatc
     7501 ctgcaaagat taaagtttcc ctgcgctctc aaggcacttt tccggcaaat aaattcgccg
     7561 cggaagtatt tggaggcggc gggcacctga acgcttccgg tggtgaaagt tacaccacgc
     7621 tcgacgaggc agtgcgtaaa tttgaagagg cattaccttt atatactgaa tttttatagc
     7681 ctgactgaac gttccggaaa ctggtaacgt tccggtttcg gctcactttc gacttaatta
     7741 tcttactgtc tgaagttttc cttcagatag actttcaaag cgagtataaa acgagcatga
     7801 acgcagtcgt tcatcaattc agatgtaaat cgaatgcgag ttgcatgtga ccataaaaaa
     7861 ataattaatt acacatgaaa tatattatta tccttggtga tggtatggca gatgaaccgg
     7921 tttcatctat cggaaatcaa acaccgttgc agtttgccaa aaaaccgtat atcgataagt
     7981 tagcctctat gggacgttgc ggaatgcttg atactattcc gcaaggttat gcccccggca
     8041 gtgaaattgc caatctttcg gtgctgggtt atgacctgaa tgaatgtttc gaaggacggg
     8101 gtacgcttga agctgcaagt atcggtgttg aaatcggtga aaatgagatg gctatgcgat
     8161 gcaacctgat ttgcattgag gacggcaaaa tcaaaaatca ttcggccggt cacatcagca
     8221 ccgaagaggc agacgtatta atccggtttc ttcaggaaaa attgggcgat gaccgggtaa
     8281 gtttccacac aggtgtttct tatcgccacc tgctgaaact taaaaacgga aataaaaaca
     8341 tcagctgcac accaccacac gatgttccgg gaacagaatt cagggatgta atgatacaac
     8401 ccttagcttc tgaagcaaaa gaaacagccg attatctgaa tgaactaacc attaagtcac
     8461 aacagctgct cgaaaatcat ccgataaatc tgaaacgtgc cgaactggga aaagacaaag
     8521 ccaacagcat ctgggtatgg tcgcccggct acaaacctca gatgaagaaa atgactgaga
     8581 tttaccctgt taatagcggt tctgtcattt cggctgtgga tttaatcaag ggaatcggaa
     8641 tatatgccgg cctgaagagt attaatgtcg aaggagcaac cggactttac accaccaatt
     8701 acgaaggcaa agtgcaggca gccattgaag ctctgaaaac cgatgatttt gtgtatctgc
     8761 acatcgaagc cagtgacgaa gccggacacg agggcgatgt ggagttgaaa atcaggacca
     8821 ttgaatacct cgacagccgt atagtaaaac cgataatgga agaattgctg acgtggaacg
     8881 agccggttac cattgcactt ctccccgacc accctactcc gtgcgccata cgtacccata
     8941 ccgctacacc ggtgcctttc gtcatttacc gtaccggaaa cacacccgat gaggttgcgc
     9001 gttacgacga attcacatgc cagtcaggaa gtttcgggtt gttaaaagga aatgaattca
     9061 ttaacgaagt gttcagaaat cagcccggca gca
//
LOCUS       DAOPYB010000122         9062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9071_length_9062_cov_9.152881, whole genome shotgun sequence.
ACCESSION   DAOPYB010000122 DAOPYB010000000
VERSION     DAOPYB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9071_length_9062_cov_9.152881"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PKH58_09380"
     CDS             <1..450
                     /locus_tag="PKH58_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89283.1"
                     /translation="FDIAANDELVRLGFVSLNDPGRGELDFNLNGTGLIANQYGQINE
                     DAYLTMPKIFVQGNNSEAGFEFAGYPNPFSGDATLTYSIPENGTVQLKVYNAIGELVG
                     LLVNESQISGKHSAVFSPYDLSAGMYTFKLEFTGLNKSKCMVLKMIH"
     gene            complement(572..4147)
                     /locus_tag="PKH58_09385"
     CDS             complement(572..4147)
                     /locus_tag="PKH58_09385"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX89284.1"
                     /translation="MIKKIFFILLFILFVFEVPAQVVINEISSSNSSVYMDEDGDYPD
                     WIEMYNGGSSVVYLGGYKISDNVSQPDKWTFPSGVNIPPYGFLTLFASDKDRKKYFNH
                     WETVVYSGDTWRYLKPFAEPDSNWRRLWSFNDGTWAQGPGGIGYGDGDDNTVISSPAT
                     SVYMRHVFNIVDTSQIASAVLHMDYDDAFVAYINGVEIARANIGVNGEPVGFSITAFA
                     EHEAHMYAGGMPDAFVIDESLLKTFLVNGNNVLAIQVHNLSPLSSDLTSRPWLSIGIK
                     NSSNNYGAVPSWFNFSASALHTNFKLSQNGETLVLSNSAGSVISQVTFGTILTDHSYG
                     CHPDGISPMKYFSIPTPGASNNLAAAYTGYTADPVFSIPGGFYAASQTLTLSTSTPGG
                     IIHYTTDGNIPKSSSPTYTSPVTFDSTVIVRARTFHPNLLEGNTLTNSYFISDSSSSL
                     LPIISLTVSPSLMFDTLTGIYVKGPNAEATVPFFGANFWQEKEIPAHIEYYDQQRQLG
                     FKQNIGIEIYGNYSRSYPQKSMKMIARDSYGNGSFDYQLFPDKDIHSFKQFIIRNSGT
                     DWNQTHMRDALIQSLSLKPTNCDVMAYQPAVVYINGKYWGVYNMREKINKDYLAENHH
                     ADPDSVDLLEYNGFVMSGSNERFIQMGLFILLNDMTNPTNFSFADSLIDLKNFADYFA
                     VETWTNNWDWLTNNVRYWRENKEGKKWRYILWDLDNGMGGAWSYVLNSLDTNLHKSTD
                     YTSLLFNKLLTNKDYKNYFINRYADLLNTLFTPQHFNTTLNSFRNRIDSEMIRHFKRW
                     GTGFSNPNWGVDGHGTYDNWKNYQLPELTAFCNNRQITARNHIQQTFDLKKQIPVTLN
                     VYPPEAGKIIINTVTIDDMPWAGIYFDSVPVRITAVPNPGYEFSFWQSMIKFPSPQAD
                     SSLTFNPDTSDVFTAYFMGQPDTSKIIFSEINYNSSPSMNTGDWVEIHNYSQWPMDIS
                     GWKFKDSQDNNIFLFPENTVLSANEYLVLCQDTTDFRSYYPGITSIFGPFDFGLNSTS
                     ESLRLFDKEMNLYLSVTFSGSYPWTADANGSGRTLELLSVNGNLDDPLNWFAGCPGGS
                     PGGPFVPCENSGFETTNPDADGTVSVYPNPVTERLIIDINPGKLINASFSVALFDMMG
                     KIVYENSDISTDEFSISNNFHPGIYFYKITSSAGYLKSGKIFFQQ"
     gene            complement(4152..5738)
                     /locus_tag="PKH58_09390"
     CDS             complement(4152..5738)
                     /locus_tag="PKH58_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89285.1"
                     /translation="MKLRFILISIICLLTTAGVFSQITLNSNNFPVGGLQIGRGYVLA
                     PVNVLGNAGPNQFYDFTNVTPVIHDSVKYYASAQTLWASFHPGATVANAEVAGNLTYV
                     YYCTSGTNAYSRTGLTLIGDFGQGMDTVHGNYSNPDTILSNQFTYGYSKTEFSVVTIP
                     NALPLVNYQIKTKRNILVDGWGQLETPLNYYPDVLRVKCAEYRYDTAFYLTSPVYTVA
                     DTQYYYNYYAKDVRHPVVKAHTDKLYNLLYFEYIYTAPVIVGCMDTMAVNYNPMANMS
                     DGSCEYCSVSYSITPDTTICEGASITLTISGGSNYLWSDGTTGSSISMLPDESSVYSA
                     YVSDSTGCWALASVEVTVDKPVTAVFWTTIDHYNTGDMIQFVNLSVNASHYNWSFDDA
                     VDGYSNQQYPQHTYTIPGMKHIELIAHNTCFTDSIMDSLKIYDGTYISNNSLSDDFNI
                     YPNPGTSLMTIEGNFDESANLEIDAIDILGRRKHMASHENISGIFRLENDLTQLSLGI
                     YVIEIIHGEKKITHKWVKMK"
     gene            5946..7445
                     /locus_tag="PKH58_09395"
     CDS             5946..7445
                     /locus_tag="PKH58_09395"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX89286.1"
                     /translation="MKKILILSAFLITFQMLKASPGDSIFNMSYIHNLYITFPYPNFY
                     DSLLYTNTTDEYIAVDINFNGEIYSQVGIKVKGNSSFNNPSQKKSFKLDFNTFVAGQD
                     IHGLKKLNFNNSFKDPSFMREKLTNEFLIDHKLPAPRVTYCNVYMNNQLWGLYTIVEE
                     IDDEFCWHWFDSNDGNLFKGDPHGTLQWKGSMNQPLYESDYELKNNSTLNDWSDLISL
                     INVINNTPDNGLQAALDQKLNTSKFVKQWVAENIFVSLDSYIGSGHNYYIYHDTVTDK
                     FQWIAWDNNESFGSFKMNLTSPQLINLDMYFISSPGARPLCEKMIQNVYYKNLYDMAY
                     CKLRDDFTNAYFDPKIDSLRPVIQASVYADPKKFYTNSKFDSSFNYNLVLPGPEGLTV
                     FGLKNFIAARAVAIQNSITNNAVDCSLQVPEHVLPQNEIILLYPNPVTTGFFIKTEIP
                     ISDIRLFDLQGRMLQSFPVNSEYIDVSKFSRGIYLLRINDVIVRKISLQ"
     gene            7855..8094
                     /locus_tag="PKH58_09400"
     CDS             7855..8094
                     /locus_tag="PKH58_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX89287.1"
                     /translation="MLNPDILSKVIKKHRKAAGLSQLQLAEMAGVGKTVIFDLEKGKE
                     TIQLDTLRKILTVLNIKVVLQSHLMNQILNTDENS"
     gene            8081..8395
                     /locus_tag="PKH58_09405"
     CDS             8081..8395
                     /locus_tag="PKH58_09405"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNX89288.1"
                     /translation="MRTAKVYVCGKEAGLLTEIIFGKEYSFIYNEGYSGLPVSLTMPV
                     SQKSFTFDHFPPFLDGLLPEGHQLEGLLKQGKVDSNDLFSQLMIVGNDTVGAVSVKEV
                     EP"
     gene            8392..>9062
                     /locus_tag="PKH58_09410"
     CDS             8392..>9062
                     /locus_tag="PKH58_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNX89289.1"
                     /translation="MKRCPITYEPSGKSLYSDKGLKMLSQNLSTLELLSYTAGEQRAE
                     AMARATKLSIQGVQPKLSAILNIKDSRFQLVDTGGKYILKPQHHIYPQLPENEDVTMR
                     MAKAAGINVPLHGMIWSKDMSLTYFIKRFDRAGHKDRIPVEDFAQLAGLSRDTKYNYT
                     MEELVLLIDEFCTFPVIEKAKLFRLVLFNFLTGNEDMHLKNFSVIRNGDKIELSPAYD
                     LVNST"
BASE COUNT         2737 a         1838 c         1902 g         2585 t
ORIGIN      
        1 ttcgatattg cagctaatga tgaactggtc aggctgggct tcgtttcgct caatgatccg
       61 ggtcgcggag agcttgattt taacctgaac ggtacgggtt tgatagctaa tcaatatgga
      121 caaataaatg aagatgccta cctgactatg ccaaagatat ttgtccaggg caacaactct
      181 gaagcgggat ttgagtttgc aggctatcct aacccattca gcggtgatgc tacgttgacg
      241 tatagcatac ctgaaaacgg gacagtgcaa ctgaaagtat ataatgctat cggcgaactg
      301 gtgggtttac ttgtaaacga atctcagatc agcggaaaac attcggcagt attttcaccc
      361 tatgacttgt ctgccggcat gtacactttc aaactcgaat ttacaggttt gaataaatca
      421 aaatgcatgg tattgaagat gatccattga ttccgaaata gattctatat cctttacaaa
      481 aaggctgtct ttaaagcaaa ggcagccttt ttttaaattg cccgattgat aaagagaaca
      541 atgtaaaagt gatatagaaa catcatcaaa attattgttg aaaaaatatt tttccacttt
      601 ttaaataccc tgccgaagag gtgatcttat aaaaatatat gccgggatga aaattgtttg
      661 aaatggaaaa ttcatctgtt gaaatatccg aattttcata aactattttt cccatcatgt
      721 caaacagtgc aacagagaag gacgcattaa tcagttttcc aggattaata tctatgataa
      781 gcctttcagt aaccggattg ggatacaccg aaacagttcc gtctgcatcg gggttggttg
      841 tttcaaaccc tgaattttcg catggaacaa aaggcccgcc tggtgaaccg cccggacagc
      901 cggcaaacca gttcaaggga tcatccaaat tgccgttcac cgaaagcagt tcgagtgtcc
      961 tgccgctacc attggcgtct gcagtccatg gatagctacc tgaaaaagtt acggaaaggt
     1021 acagattcat ttctttatca aataaacgca aactctctga agtagaattc aggccgaaat
     1081 caaaaggtcc gaaaatgctg gtaattcccg gataataaga tctgaaatct gtagtatcct
     1141 ggcaaagcac taaatattca ttcgctgata ataccgtatt ttcaggaaac aggaagatat
     1201 tgttatcctg gctgtccttg aatttccagc ctgaaatatc cataggccac tggctgtaat
     1261 tgtgtatttc aacccaatca cccgtattca tcgatggaga ggaattgtaa ttgatctcac
     1321 tgaaaataat ttttgaagta tccggctgtc ccataaaata cgcagtaaac acatcagatg
     1381 tatcaggatt aaatgttaat gaggaatcgg cctgcggcga gggaaattta atcatggact
     1441 gccagaaact aaattcataa ccgggattcg gaacagcagt aattcttacc gggacactat
     1501 cgaaatatat ccccgcccat ggcatatcgt caatggtaac cgtgtttata ataatttttc
     1561 cggcttcagg cggatataca ttcagcgtaa cggggatttg tttttttaaa tcaaaggttt
     1621 gctggatatg attccgggca gtgatctggc ggttattgca gaaggcagtt agttccggca
     1681 actggtaatt tttccagtta tcatatgtgc cgtggccgtc gacgccccag ttagggttgc
     1741 taaatcctgt tccccatcgc ttgaaatgcc ggatcatttc actgtcgatc ctgttcctga
     1801 aactgttcag cgttgtattg aaatgctgag gggtaaacag tgtgttgagc aggtcggcat
     1861 acctgtttat aaaataattt ttataatctt tattggtaag cagcttgttg aataggagtg
     1921 aggtataatc ggtgctttta tgcaggttgg tatccaatga atttaagaca tacgaccagg
     1981 ctcctcccat gccgttatcc aggtcccaca atatatagcg ccacttttta ccctctttgt
     2041 tttctctcca gtaacgtacg ttattggtca gccagtccca gttgtttgtc caggtttcaa
     2101 cggcaaaata atctgcaaaa tttttaaggt ctatcagcga gtccgcaaag gagaagtttg
     2161 tgggatttgt catgtcattt agaagtatga ataaccccat ttgaataaac cgttcgttcg
     2221 aacccgacat cacaaaccca ttatattcca aaaggtcaac agagtcgggg tctgcatggt
     2281 gattttcggc cagataatct ttattgatct tttcacgcat attatagacg ccccagtatt
     2341 tcccgttgat ataaaccacg gcaggctgat aagccatcac atcacaattg gtcggtttta
     2401 gcgaaaggct ttgtatcaga gcgtcgcgca tgtgtgtctg gttccagtcg gtgccggaat
     2461 tacgaatgat aaactgtttg aacgaatgaa tatctttgtc aggaaacaac tggtaatcga
     2521 aggatccgtt cccgtaactg tcgcgtgcaa tcattttcat gcttttttgg ggatatgacc
     2581 ttgagtaatt gccgtaaatt tctattccaa tattttgttt gaagccaagc tggcgttgct
     2641 ggtcataata ttcaatatgt gcaggaattt ctttttcctg ccagaaatta gcgccgaaaa
     2701 aaggaacagt agcctcggca ttcgggcctt tgacatagat cccggtgagc gtatcaaaca
     2761 tcagtgatgg tgaaacagtc agcgaaatta tcggaagcaa ggacgatgat gagtctgaaa
     2821 taaaatatga atttgtcaat gtatttcctt ccagaagatt gggatgaaaa gttcgtgcgc
     2881 gtacaatcac tgtagaatca aaggtaacag gggaagtgta agtgggagag gaacttttag
     2941 gaatgttgcc atcagtggtg taatgaatga tccctcctgg agttgatgtg gaaagggtta
     3001 atgtctgtga agcagcataa aaccctccgg gtatcgagaa tacaggatca gcagtgtagc
     3061 cggtgtatgc ggccgcaagg ttgttggaag cgcccggcgt gggtatgctg aagtatttca
     3121 tcggcgaaat cccatcagga tggcatccgt aagaatgatc tgtaagtatc gttccgaatg
     3181 ttacctgact gataaccgaa cctgcactgt ttgataaaac aagcgtttcg ccattctgcg
     3241 ataatttaaa gttagtatgc agggcagaag cgctgaaatt gaaccacgaa ggaacggcgc
     3301 catagttatt cgaactgttt ttaattccta tggaaagcca tgggcgggaa gtcagatcag
     3361 atgataatgg acttaaatta tgaacttgta tcgccaacac attattcccg ttcactaaaa
     3421 aagtcttcag caaactttcg tcaatcacaa aagcatcagg cattccgccg gcatacatat
     3481 gggcctcgtg ttctgcaaaa gcagttattg aaaatcctac aggttcgccg ttaacaccga
     3541 tgttggcgcg tgcgatctca acaccgttga tataggcgac aaaggcgtca tcgtaatcca
     3601 tatgcaatac cgcagaagct atctgggatg tatcaacaat attaaaaaca tgccgcatat
     3661 atacggaggt cgccggagag gaaatcacgg tgttgtcatc gccgtcgccg tacccgatcc
     3721 cgcccgggcc ttgcgcccat gtgccgtcat tgaacgacca gagccttctc cagtttgaat
     3781 caggctctgc aaatggtttt aaataacgcc aggtgtcccc ggaataaact acagtttccc
     3841 agtgattgaa atacttttta cggtctttat ccgaggcgaa aagcgtaaga aaaccatagg
     3901 gcggtatatt gacaccggaa ggaaaggtcc atttgtcagg ctggctgacg ttatcactga
     3961 tcttataacc gcccaggtaa actacagaag aacctccgtt atacatctcg atccagtcgg
     4021 gataatcccc gtcttcatcc atatacacag aactgttgga ggacgaaatc tcattgataa
     4081 ccacctgagc aggtacttca aaaacgaaaa gaataaaaag cagaataaaa aatatttttt
     4141 ttatcatctg tttatttcat tttgacccat ttatgtgtaa tttttttctc tccatgaatg
     4201 atttcgatca cgtaaatacc cagtgacaac tgtgttagat cattttccag cctgaaaatt
     4261 ccggaaatat tttcgtgaga tgccatgtgt tttctgcgcc ccaaaatatc aatagcatca
     4321 atttcaagat ttgcagattc atcaaagttg ccttcaatgg tcataaggct tgttccggga
     4381 ttagggtaaa tattgaaatc atccgaaagg ctattgttgc ttatgtatgt gccgtcataa
     4441 attttcagcg aatccataat gctgtccgta aaacaggtgt tatgggcgat caattcaatg
     4501 tgtttcattc cggggatggt gtatgtatgt tgcggatatt gctggttact gtagccatcc
     4561 acggcatcat caaaactcca gttgtagtga gatgcgttga ctgagaggtt aacaaactgt
     4621 atcatatcgc cggtattata atgatcgata gtggtccaaa acactgcagt aacgggtttg
     4681 tcaacggtta cttccacaga tgcaagagcc cagcaacctg tgctgtcact gacataagcg
     4741 ctgtaaaccg aactttcatc agggagcatc gatatggatg agccggtagt tccgtcggac
     4801 cataaataat tgcttccccc actaatggtc aaggtaattg acgccccctc gcaaatagta
     4861 gtatcaggag taattgaata actaaccgaa caatattcgc aactgccatc ggacatgttt
     4921 gccattgggt tgtaatttac tgccatggta tccatgcatc ccacaataac cggcgctgta
     4981 tagatgtatt caaaataaag tagattatac agtttgtcgg tatgcgcttt tacaacagga
     5041 tggcggacat ctttagcgta ataattataa taatattgag tgtcggcaac ggtatatact
     5101 ggtgaagtta agtaaaatgc agtgtcatac cggtattcgg cacatttaac ccgtagcaca
     5161 tccgggtaat agttcagcgg cgtttcgagc tggccccaac cgtccacgag tatattcctt
     5221 tttgttttta tctggtaatt aacaagtgga agagcattag gaatggtgac tacagaaaat
     5281 tcggtttttg aataaccata ggtgaattga ttacttaata ttgtatccgg attactataa
     5341 ttgccatgaa cagtatccat gccctgcccg aaatcaccga tgagcgtaag gcctgtcctg
     5401 ctataagcgt tggtgcctga agtacaataa taaacgtagg tcaggttgcc tgcaacttct
     5461 gcgttggcaa ctgtggcccc gggatgaaat gatgcccaca gcgtctgggc agaggcatag
     5521 tattttacgc tgtcgtgaat aacaggcgtt acattggtga aatcataaaa ctggttaggc
     5581 cccgcgttgc ctaaaacatt caccggggcc aaaacataac cgcgcccgat ctgtaaaccg
     5641 ccaacaggga aattattact gttgagtgtg atttgggaaa acacacctgc tgtagtgagc
     5701 agacaaatta ttgatataag aataaatctg agtttcatcg gttttaccca aatattatta
     5761 tacaaattaa ataaaaaaaa atcaagaaat caataataat caataataag accgggctta
     5821 tcccttaaaa aagtccagga acctgaaatt gatgccttta attgaagact gccggaaatt
     5881 tcatatattt gctaaagtaa aagtgacccc tgctaagagt tattaaccca taatcattct
     5941 cttccatgaa aaaaatcctt atcctgtcgg catttcttat taccttccaa atgcttaaag
     6001 ccagccccgg tgattctatc ttcaacatgt cctacattca taatctttat atcacctttc
     6061 cgtatcccaa tttctatgac tccctgctat acactaacac tacagacgaa tacattgccg
     6121 tggacatcaa tttcaatgga gagatataca gccaggttgg cataaaagta aaaggtaact
     6181 catcattcaa caatcccagt cagaagaaat ctttcaaact tgatttcaac acttttgttg
     6241 cggggcagga tattcatggc cttaaaaaac tcaactttaa caactccttc aaagatccgt
     6301 cctttatgcg cgaaaaactc accaatgaat tcctcataga ccacaaactg cctgccccgc
     6361 gcgtaacata ctgcaatgta tacatgaaca accagctttg ggggctttat actattgtag
     6421 aagaaattga tgatgagttt tgctggcatt ggtttgacag caacgatggc aacctcttta
     6481 aaggcgaccc gcatggcacc ctgcagtgga agggcagtat gaatcagccg ctttacgaat
     6541 ccgattatga acttaagaac aacagcaccc tgaacgattg gagcgatctg atatctttga
     6601 taaatgtgat caacaacact cctgataacg gcttgcaggc tgccctggat cagaaactga
     6661 atacttccaa gtttgtaaaa caatgggtgg ccgaaaacat ttttgtaagc ctggactcat
     6721 acatcggcag cggacacaat tattatattt accacgacac agtcaccgat aagtttcagt
     6781 ggatagcctg ggataacaac gaatcattcg gaagctttaa aatgaacctc accagccctc
     6841 aactgataaa cctcgacatg tactttatca gctcgcccgg cgccaggccc ctttgtgaaa
     6901 aaatgataca gaatgtatat tataaaaacc tttatgatat ggcttattgc aaactgaggg
     6961 atgattttac caacgcatat tttgatccca aaattgactc cttgcggcct gtcattcaag
     7021 ccagtgttta tgccgatccg aaaaaattct acactaattc aaagttcgac tcaagtttta
     7081 attataatct ggttttaccc gggccggaag gattaactgt ttttggatta aaaaacttta
     7141 tcgcggcgcg tgcagttgct attcaaaaca gcataaccaa caacgctgtg gattgctcgt
     7201 tacaggtgcc tgagcatgtt ttgccgcaaa atgaaatcat ccttctttat ccaaaccctg
     7261 tcactaccgg tttttttatt aaaactgaaa ttcccatctc agacatcagg ctcttcgacc
     7321 ttcagggacg aatgctccaa tcctttccgg tgaattccga atatatcgat gtgagcaaat
     7381 tcagccgggg aatatatttg ctgaggataa acgatgtaat agtcaggaaa attagcttac
     7441 aataaaatgg ttttaccatt ctttgtcact cctgtttgat acagcacaaa tcttcttgtt
     7501 ttttgaacag gatattttca ttttctgaaa ctggctgtac tccttgcttg gcaagcataa
     7561 catatgctgt gaggtaaata aaactgtaaa ggccttggat ttttattgat tttcagattt
     7621 gtaatagttt agttccgtga agataattca cgattcaggc aaatagtatg aatgtacccg
     7681 aaaaggtata attgtaaaat tatttcaaaa aattactgct aaagcggtaa atatgatata
     7741 ataatatcaa aaagtacaga aataaattaa aaaatactta tatagcggta atattttgaa
     7801 tattccttga cttacttagt gaattgttgt atctttgccg ctaaaacggt aattatgctt
     7861 aacccggata ttttatcaaa agtaattaag aagcacagaa aagcagccgg actaagccag
     7921 ctccaactgg cagagatggc aggtgtcggc aaaactgtga tctttgatct tgaaaaaggt
     7981 aaagaaacaa ttcagcttga taccttaagg aagattctca ctgtattgaa tataaaggtt
     8041 gtcttgcaaa gtcatcttat gaaccaaata ttgaatacag atgagaacag ctaaagttta
     8101 tgtatgcgga aaggaggcag ggctgcttac tgaaattatt ttcgggaaag agtacagttt
     8161 catatataat gaaggatact ccgggttgcc cgtctctctt accatgccgg tcagtcagaa
     8221 atcttttaca ttcgatcatt ttccgccatt ccttgatggg cttttaccgg aaggacacca
     8281 gttggaaggc ttgttaaaac agggaaaagt tgactcgaat gatctttttt cacaattgat
     8341 gatagttggc aacgatactg ttggtgcggt tagcgtgaag gaggttgaac catgaaacgc
     8401 tgtccgataa cctatgaacc tagcggtaaa tcactttaca gtgacaaagg attaaaaatg
     8461 ttgtcgcaaa atctgagtac tcttgaattg ttatcctata ccgctggtga gcagagagcc
     8521 gaggctatgg ccagggcaac caaattatct atacagggcg ttcaaccaaa actcagtgca
     8581 atcctaaata ttaaagattc caggtttcaa ctggttgata ccggcggaaa atacatccta
     8641 aagccacagc atcatattta tcctcagctc cctgaaaacg aagatgtgac catgcgcatg
     8701 gcaaaggcag ctggaattaa cgttcccttg catggcatga tctggtcaaa agatatgtcg
     8761 ctgacttatt ttataaaacg tttcgacagg gccgggcata aggatagaat tccggttgaa
     8821 gattttgcac aattggcggg actttcaaga gatacaaagt acaattatac gatggaggaa
     8881 cttgttttac tcattgacga gttttgtact ttcccagtaa ttgagaaggc aaaattgttc
     8941 cggcttgtgc tttttaattt tcttaccgga aatgaagata tgcacctcaa aaatttttct
     9001 gtgatcagga atggagataa aattgagttg tctccggcct acgacctggt aaattcaacc
     9061 at
//
LOCUS       DAOPYB010000123         9032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9113_length_9032_cov_9.228473, whole genome shotgun sequence.
ACCESSION   DAOPYB010000123 DAOPYB010000000
VERSION     DAOPYB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9113_length_9032_cov_9.228473"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..1568)
                     /locus_tag="PKH58_09415"
     CDS             complement(468..1568)
                     /locus_tag="PKH58_09415"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HNX89290.1"
                     /translation="MLKKQIFTLFLLLTSICFSQSSYKMLVGTYTRGTTSKGIYSLEF
                     SGKGKLLSQKLLATSDNPSYLALSPDGKNVYAVNETGTESTVSAFAFDREHETLTFIN
                     KVNAGGADPCFITVTDNHVFTANYSGGSISVFGRKAGGAVTDTLKVIRHQRKNFGRGR
                     FGPSNAHQVLFSPDRKVMIATNLGTDHVFSYSYNPDDNKNTLKYIDQISVRKHSGPRH
                     TVFSADGRYLYLVQELDAGVTVFSVNEEGRLRIIQETTLVTDSTKKNGAADIHLSPDG
                     KYLYATNRGEANNISCFKVNKNGSIAFKAQYPTEGNGPRNFTITPDGKFVFVANQRTN
                     NITVFSRNKYTGKLTLTDNKVELGAPVCLLQY"
     gene            complement(1775..3184)
                     /locus_tag="PKH58_09420"
     CDS             complement(1775..3184)
                     /locus_tag="PKH58_09420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX89291.1"
                     /translation="MKVKFIGAAREVTGSKTLITTDDGKKILLDCGMFQGKGLETDEL
                     NRKTGINPAELNHIILSHAHIDHSGLIPYFYKLGFRGSVICTTATRNLCSIMLPDSGH
                     IQEADTVWFNKKRTRQNLPPVNPIYDVQDAEDALKLFIAVDYNRWFYINDKVKVKFTN
                     TGHMLGSGVVNIEVKENDRKIHLAYTGDIGRPHNRILSGPVPFPQCDYLITESTYGNR
                     LHETEKQTEEDFLKIITDTCVVNKGKLIIPSFAIGRTQEVVFVLNKFFNDGLLPRIKV
                     YVDSPLAVNATDIYRMHLDCMNEDVKRELKEGDGDPFGFKDLIYIKKVEQSKELNDLK
                     EPCIIISASGMAEAGRIKHHLANNISNPLNTILMVGYCSPTSLGARIQEPGLKEISIF
                     GEVHEVKARIARMEAFSGHGDYNEMKSFLECQDSALIKKTFLVHGEYEVQQFYKDILN
                     KDGFENIEIPAYLNEFELN"
     gene            3402..4637
                     /locus_tag="PKH58_09425"
     CDS             3402..4637
                     /locus_tag="PKH58_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX89292.1"
                     /translation="MDTNHVRVSLEVDLEEKLYKMLEGEMIEKIIAEANYEPEENIWK
                     NILEGHSFKVTHEMAPKLFDLFQDVKEKLRYSKPIDFYITNSAELNAFALTRIEKGEA
                     DIININSGLISMFDDEELKFVIGHEIGHLVTRYARVVKLIDFIFPGLEDIPLILAHKI
                     ELWQKVSELTADRFGYMASPSVEKCVSGFFKMASGLNTDRIKFDYQAYIRDNEKILDY
                     FSENRNLLSHPVNPLRIKAIELFGESKLFAAISQGKDLEHDVELDRKMQKLIEILITI
                     SSSPMDNQRKLFVASAGILMAQIDKEMDEEEYEEILEALSMYTVFPKEFLSDMIKEDV
                     PKIFQDSIKNIISQNPAERTQLLEYMASVAFCDSNMFQNEMDFLFETGERMLGLTRRE
                     TAQIMAATLQQGFIPELYK"
     gene            complement(4835..6067)
                     /locus_tag="PKH58_09430"
     CDS             complement(4835..6067)
                     /locus_tag="PKH58_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89293.1"
                     /translation="MNRPILNIRQLIIFIFLISISSYASGTNKTGQQTSDTVPQQTIP
                     ILAWYSIPEAFSTTERFGELAETGINLHFSFYSDANEVQKALDLAEEHNLKVIISCPE
                     LETETKKTVRRFMYHPALAGYYLQDEPTATQFGKLREWVKHVRAADSMHYCYINLFPN
                     DVRPRKLEASGYRSYVSRFLKEVPVPFLSFDHYPVVGETLRPIWYENLEIIAAASRKH
                     NLPFWAFALTTAHDYYPVPDLAELRLQIYSNLAYGAQGIQYFTYWTPPVDAYDFHHGP
                     ITLDGKRTNAYELIKAMNTEIRALSGVFLGATVISVRHTGTSIPKGTKRFKKLPAPFL
                     KIDTGNSGAVVSDLKNGDKKYLIIVNRSFLETMEVRIELNSNSVRQVLKNGKMHLLPS
                     GLSFFQVEAGDVLILKYE"
     gene            complement(6249..>9032)
                     /locus_tag="PKH58_09435"
     CDS             complement(6249..>9032)
                     /locus_tag="PKH58_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89294.1"
                     /translation="NTWQRYDGTSWVTPTAAQGYPGQYTGTGAINIRSGNTIYANFIY
                     PSTLTPIQLTVIGINNNNPFTAYTTPYPVGIITVQSDAILVLQGSSGGSGNDTFYMKT
                     SELNVNSGGVLYFNGNKVNLALPANSVIEVCSGGLYGSSSDGNANQNIFIGNQWYAVG
                     NSNKTQFTFDEIMYGCRGTLEAEIGYTSPICVGNTISLAGSYSGTIDTSPSYSWSVVS
                     PGGTTTTSSQMNYSLIGSEVGIYTVTLTVTTTRTNKTTYTFTNSKTVTITVLPNASLS
                     LTSGTATANQTVCINKPLANITYTATGATGVTFSGLPAGVTGTYSDGVATISGTPTVA
                     GTFNYTVTTTGTCLAAQQSGTISANQSYWVGIVSNDWNTEANWTCGIPPTGADVVFAA
                     STNSYGSMALRDLHLDTQRTIGSLVNASNKSLVIKPATTLIINNTAPANDNPAKIQIQ
                     AAPDKVNGCLIFTNPLSNPNVNATVQMYTTGYKGATQWTWTDPDGKPYSGYYRWQYFG
                     VPVESTSTLVSNSSLWGSYIRKYNEGLELNSYYQKWSNLGNYDQLLPFSGYEITQGSP
                     KIISFSGKIVTGDRTLTLTKSEISGTKNYGSGYNIYGNSFTAAIDVSKLSFGAGVQQT
                     VYLYNTGSLQDWGSQQNQSGNIAGAYYAIPVNTAPAMGSIPSMQGFLLITGTNGATVT
                     IPYNSVTSAATANTSQQRVRQKISANEELSRMTVDVAGENSFDRVWIFSQPGTTRGFD
                     NGWDGEKILQTNGVALYAEEENRKYQVSTTENLENIWLTFQAGATTDYTLTINKSSLK
                     DNNNLILFDSIANTIINLSDSITVYPFVAFNNGSPEKRFVFKVITDIRDFENNKAVKL
                     RAFTANNAIKIQNLTEENATYSISDEFGVFKFSGAIGPNSTISPNIKFESGVYFVFIK
                     TKNQNIIEKVIIK"
BASE COUNT         2376 a         1859 c         2040 g         2757 t
ORIGIN      
        1 ttactatgtg ctggttatca gggatggaaa aacttaccgc aggcagctga tggtgaaata
       61 aaacggacta caaacacttc ttaaaaataa aaaagaggca tttccttcat cgggagttgc
      121 ctcttttgct attttctttt attctcgggg tgactttgag tcaccccgag aatttcagag
      181 acattgcaaa ttaaagcctt gtagagggtg catcaggatt aaaaagtgta ttttttcagc
      241 cgcaaaactg cggcaacaca ttatcagttg atattgcctt cccgcaaaac tgcggcggaa
      301 aaatactccc ggctgaacac ttttcaagat atttcaacga aaaattttgt tattaaacga
      361 gataaaataa actaaccggt gaattaacag caaagaaaag attaatgaaa aactcccgaa
      421 ttcagatgaa tttcgggagc ttttcattaa tacctgagta agtaaattta gtactgcaac
      481 aaacaaaccg gagctccaag ttccacttta ttgtccgtta atgtaagttt acccgtgtat
      541 ttattccggg agaaaacggt gatgttattg gtgcgctgat ttgctacaaa gacaaatttt
      601 ccatcgggtg tgatggtgaa attacggggc ccgttgcctt ctgtcggata ctgcgctttg
      661 aaagcgatgc tgccgttctt atttactttg aagcagctga tgttgttcgc ttctccccgg
      721 ttggtggcat agaggtattt cccgtcgggg ctgaggtgga tgtcggcagc gccgtttttc
      781 ttcgtgctgt cggttaccaa cgttgtttcc tgaataattc tcagccgtcc ctcttcgttc
      841 accgagaata cagtcacacc ggcgtcaagc tcctgcacca gataaagata cctgccgtca
      901 gcgctgaaaa ccgtatgccg ggggccgctg tgctttctca ccgaaatctg atcaatgtac
      961 ttaagcgtat tcttattgtc gtcggggttg taggaatagc tgaacacatg gtcagtgccc
     1021 aggttggtgg ctatcatcac cttgcggtcg ggtgaaaata gtacctgatg agcattgctc
     1081 ggcccgaaac gtccacgtcc gaaattcttc cgctgatgtc tgatgacttt cagggtgtcg
     1141 gtaaccgcgc ctccggcttt tctgccgaat actgaaatac ttcctcccga atagttggcc
     1201 gtgaaaacat gattgtcggt aacggtaatg aagcaggggt cagctccacc cgcattgact
     1261 ttattgatga aagtcagggt ttcgtgctcc cggtcgaatg caaacgcgct caccgtactt
     1321 tcggttcccg tttcgttcac ggcataaaca ttttttccat ccggcgacag agccagatag
     1381 ctcgggttgt cgcttgttgc cagtagcttt tgcgaaagga gtttgccttt gcccgagaat
     1441 tcaagcgaat atatcccctt gctggtagtg ccgcgcgtat acgtcccgac aagcatttta
     1501 taactgctct gcgaaaaaca aattgacgtc agtaagagaa agagagtaaa tatctgtttt
     1561 tttagcataa ttatatacgt ttgatgttaa ttaaaagccg ggactttgag actttgagaa
     1621 ctggtgacat tgagactttg atagcaaaga cttatagaac caggacattg agacttggtg
     1681 tgacgggact tttgaaccat taatacaata acttaataac caattacaat ttacggattt
     1741 tcaaattggc acattggcca tcttcaagag ctcactaatt caattcaaat tcattcaggt
     1801 aagcgggtat ttctatattt tcaaagccgt ccttgttcaa gatatctttg tagaattgct
     1861 ggacttcgta ttcgccgtga acgaggaatg tttttttaat cagcgcactg tcctgacatt
     1921 cgaggaaact cttcatttcg ttgtagtcgc catgaccgga gaaagcttcc atccgtgcaa
     1981 tccgcgcttt cacttcgtgc acttcgccaa agattgaaat ttccttcaga cccggttcct
     2041 gaatacgtgc accgagtgaa gtgggggagc agtatccaac catcagaatt gtattcagcg
     2101 gattggatat gttgttggcc aggtggtgct tgatgcgtcc ggcttcagcc attcccgatg
     2161 ccgagatgat gatacagggt tctttcaggt cattcagttc ttttgattgt tccacttttt
     2221 tgatgtaaat gaggtctttg aaaccaaatg gatcaccatc tccttccttc agttctcgtt
     2281 ttacatcttc gttcatgcag tccaagtgca ttctgtaaat atccgtggca ttgaccgcga
     2341 gcggactatc cacatatact tttatgcgtg gcagcaatcc gtcgttgaag aatttgttga
     2401 gtacgaaaac cacttcctgc gtgcgtccga tggcaaatga agggataatc agttttccct
     2461 tgttgacaac gcaggtatcg gtaatgattt taagaaaatc ctcttcggtt tgtttctcgg
     2521 tttcgtggag gcggtttccg taggtggatt cggtaatgag gtaatcacat tgcgggaagg
     2581 gaacgggacc gctcagaata cggttgtgag gtctgccgat atcaccggtg tatgccaggt
     2641 gaatttttct atcgttttct ttgacttcga tgtttaccac gccgctcccc agcatgtggc
     2701 cggtattggt gaatttaacc ttcactttat cgttgatata gaaccagcgg ttgtaatcca
     2761 cggcaataaa caacttcagc gcgtcttccg cgtcctgaac atcgtaaata ggatttacag
     2821 gcggcagatt ttgtcttgtc cgtttcttgt tgaaccacac ggtatcggct tcctgtatat
     2881 gtccgctgtc ggggagcatg atggagcaga gattacgggt ggcggtagtg caaatcactg
     2941 aaccccggaa gcccagtttg taaaagtagg ggattagccc cgaatgatcg atatgagcat
     3001 gtgaaaggat gatatggttt aattcggcag gatttatacc ggtcttccgg ttcagttcgt
     3061 cggtttcaag tcctttaccc tgaaacatac cgcagtcaag cagtattttt ttgccatcgt
     3121 cagttgtaat cagcgttttg ctgcctgtaa cttcgcgtgc tgcgccaata aattttacct
     3181 tcatattttt gtgtgtttat aggatttgct ttcgaatacc acaaaaatag gttttttaat
     3241 cggaagttaa catcacgaag cgaaatatct tttttttcag ataaaaattt ccagcaaccg
     3301 gaatattaat tttatcttta tcatccgaaa caaagttgaa ttcaaagcag ttagtataat
     3361 gacagtggga taaatcctaa acaaataatc caataaaaca tatggacacc aatcatgtac
     3421 gtgtaagtct ggaagttgat ctggaagaaa aactttacaa gatgctggaa ggtgaaatga
     3481 ttgaaaaaat cattgctgaa gccaattacg aaccggaaga aaatatctgg aagaatattc
     3541 tcgagggtca cagttttaag gtgactcacg agatggcacc caaacttttc gatttatttc
     3601 aggatgtaaa ggaaaaactc agatacagca aacccattga cttttatatt accaattcgg
     3661 ccgaactcaa cgccttcgcg ctgacccgca tcgaaaaggg tgaggcagac attatcaata
     3721 tcaactcagg cctgatcagc atgttcgatg atgaggaact aaaatttgtt atcggtcatg
     3781 agattggtca tctggttaca cgctacgcgc gggtagtgaa actgattgat ttcatatttc
     3841 ccggactgga agacataccc cttatcctgg ctcataaaat tgaactctgg cagaaggtgt
     3901 ccgaacttac tgccgaccgc tttggttaca tggcaagtcc gagtgtggag aagtgtgtat
     3961 cgggattttt caagatggct tctggtctca ataccgaccg gatcaagttc gattatcagg
     4021 cttacatccg cgataatgaa aaaatactcg actacttcag cgaaaaccgg aacctgctca
     4081 gtcatccggt gaatccgctt cggataaaag cgatcgaact cttcggcgaa tcgaaattgt
     4141 ttgccgccat ttctcaggga aaagacctgg aacacgatgt ggaactggac cggaaaatgc
     4201 aaaagctgat agaaattctg attaccatca gttcatcgcc aatggataat cagcgaaagt
     4261 tattcgtagc ctcagccgga attctaatgg cacagataga taaagaaatg gacgaagagg
     4321 aatatgagga aattctggaa gcgctgagca tgtacacggt attcccgaaa gaatttctga
     4381 gtgatatgat aaaagaggat gtgccgaaga tatttcagga ttcaattaaa aatataatca
     4441 gccagaatcc tgccgaacga acccagctcc tggaatacat ggccagtgtt gctttctgcg
     4501 acagcaatat gtttcagaat gagatggatt ttctgtttga gaccggtgaa agaatgctgg
     4561 ggcttacgcg gcgtgaaacg gcacaaatca tggctgcaac cttgcagcag ggttttattc
     4621 cggaactgta taaataaagt gattgttgtt tggtcaattt aaaagcaatc gctccggtat
     4681 tttccggagc gatttgcgtg tagtgttatt taagtgcctt ctgtttttca gacagaagca
     4741 ttttctccac taacctgtac agttttttgt aatggtttgg caatgcagtt tctaaaaaat
     4801 cgttttgtaa ttcttttttt ccggatataa aatattattc atatttgagt attaacacat
     4861 caccagcttc cacctgaaaa aaggataaac ctgaaggcag aagatgcatt tttccatttt
     4921 ttaatacctg cctgacagag tttgagttta attctattcg tacttccatg gtttccagaa
     4981 aactacggtt aacgattatc aggtactttt tatctccgtt tttcagatca gaaaccactg
     5041 cgcccgagtt accggtatct attttcagaa aaggagcggg cagcttttta aatcgtttgg
     5101 tgcctttggg aatgctagtc ccggtatgac gtaccgatat gaccgttgcc cccagaaata
     5161 cgcccgaaag cgctctgatt tcagtattca tcgcttttat tagttcgtat gcattggttc
     5221 gctttccgtc aagggtgatg ggaccgtgat gaaaatcata agcatcgacc gggggtgtcc
     5281 aataggtaaa gtactgaatg ccctgagcac cgtatgccag gttactgtag atttgaagcc
     5341 gcaattccgc cagatcgggg acggggtaat aatcgtgggc ggtagttagg gcaaaagccc
     5401 agaaaggcag gttgtgtttg cgtgaggcgg cagcaataat ttccagattt tcataccaga
     5461 tgggtcgcaa agtctcacca acaaccggat aatggtcgaa cgaaagaaac gggaccggaa
     5521 cttctttcag gaaacggcta acatagctgc gatagccaga cgcttcgagt ttacggggtc
     5581 ttacatcgtt gggaaataaa ttgatatagc aataatgcat gctgtccgct gcacgaacgt
     5641 gttttaccca ttcacggagt ttgccgaatt gggtggctgt cggctcgtcc tgcagataat
     5701 atcctgccag cgccggatga tacatgaacc gtctgactgt ttttttggtt tcggtttcca
     5761 gttcaggaca cgatatgatt actttcagat tgtgctcttc cgccagatcc aatgcttttt
     5821 gtacctcatt agcatcggaa taaaatgaaa aatggagatt gatacctgtt tctgcaagtt
     5881 cgccaaatcg ttcagtggta ctgaatgctt caggtatact gtaccaggct aatatgggta
     5941 ttgtttgctg aggaacggtg tcactggttt gttgcccggt tttatttgta cccgatgcat
     6001 acgaacttat cgagatcaaa aagataaaaa tgatgagctg tcttatgttt agaataggtc
     6061 tgttcatttg aattcctgat tattgcagag gactaatatt gcgaggttta acttcagccg
     6121 gtattatcta ttttcaaaaa cacaagatac aaaaaaaacc gggatattca attatgtgtt
     6181 tataaaagtt taataatcag aaagagcagc tttttttaag gctgctcttt ctgattatcg
     6241 cgtgatattt attttatgat tactttttcg attatatttt gatttttcgt tttaataaat
     6301 acaaaataga cgcctgattc gaacttaata ttaggcgaga ttgttgagtt tggccctatc
     6361 gctccactga acttgaaaac accaaattca tcacttatgc tgtatgtcgc gttttcttct
     6421 gtcaggtttt gtattttaat agcattgttc gcggtgaatg ctcttaattt tacagcttta
     6481 ttattttcaa agtctctgat gtctgttatc actttaaata cgaacctttt ttccggactt
     6541 ccattattga atgctacaaa cggatagacc gtaatgctgt cggataggtt gatgatcgta
     6601 ttagcaatgg agtcaaacaa aattaaattg ttgttgtctt ttaatgacga tttattgata
     6661 gtaagtgtat aatcggttgt agctcctgcc tggaatgtga gccatatatt ttccagattt
     6721 tcggttgtac ttacctgata ttttctgttt tcttcttccg cgtacaatgc cactccgttt
     6781 gtttgcagga ttttttcacc atcccagccg ttatcaaatc cgcgggtggt acctggctga
     6841 ctgaaaatcc ataccctgtc aaaactattc tcacctgcaa catcaacagt catccgggat
     6901 aattcttcgt tggctgatat tttctgcctt acccgctgct gtgaggtatt agccgtggca
     6961 gctgatgtta cactgttgta agggatggta actgttgctc cgtttgtgcc cgttataagc
     7021 agaaatccct gcatcgatgg aatggaaccc attgcaggtg ccgtattgac cggaatggca
     7081 tagtaagcac cggcgatatt ccctgattga ttttgttgac tgccccagtc ctgaagactt
     7141 cctgtattat aaagatatac cgtctgctgt actcctgctc caaagcttaa tttagaaaca
     7201 tcaatagcag ccgtaaatga atttccatag atattatatc cagaaccata attctttgta
     7261 cctgatattt cagatttggt taaagtcagc gtacgatcgc cggttacaat ttttccgctg
     7321 aaagagatga tttttggact gccttgtgtt atttcatatc cgctgaaggg aagtaattgg
     7381 tcataatttc cgaggttact ccatttttgg tagtaactgt tgagttcaag tccctcattg
     7441 tatttccgga tatacgatcc ccacagcgag ctgtttgata caagagttga ggtgctttcc
     7501 accggaacgc cgaaatattg ccagcggtag tatccggaat atggttttcc atccggatcg
     7561 gtccaggtcc attgtgttgc acctttgtat cctgtagtgt acatctgtac ggtggcattc
     7621 acattcgggt ttgatagtgg atttgtaaat atcaggcagc cgttaacttt atcgggtgct
     7681 gcctgaatct ggatttttgc aggattgtca ttggccggag ctgtgttatt gatgataagc
     7741 gttgtcgcgg gtttaataac caagctcttg ttgcttgcat ttaccaggct tccgatggtt
     7801 ctttgggtat cgaggtgtaa atcgcgtaat gccattgaac cgtaactgtt ggtagatgct
     7861 gcaaatacta catcggctcc tgttggagga ataccacagg tccagttggc ctctgtattc
     7921 cagtcattac ttactatacc cacccagtag gattggtttg cacttatggt gcccgattgc
     7981 tgagcagcta aacaggttcc ggtagttgtg actgtataat taaatgttcc tgctacagtg
     8041 ggcgtaccgc ttatggttgc cactccgtcc gaataagttc cggtaactcc ggcgggcaag
     8101 ccgctgaatg tcactccggt agcgccggtt gctgtataag tgatattggc cagcggtttg
     8161 ttaatacata ccgtttggtt ggcagtggca gtacccgatg ttaatgacaa tgaagcattc
     8221 ggtaatacag taattgtaac agtcttgctg tttgtgaaag tataggttgt tttgttggtt
     8281 cttgttgttg taacggtgag tgtcactgta taaatgccca cttcactacc aattaaactg
     8341 taattcatct gactggaggt ggtggtggtg ccacctggtg atacgacaga ccatgaatac
     8401 gagggacttg tgtcaatcgt accgctgtaa cttccggcta aagatatcgt attccccaca
     8461 catatcggtg aagtgtatcc tatttctgct tccagcgttc ccctgcaacc gtacattatc
     8521 tcatcaaacg taaactgtgt cttgttgctg ttacctactg cataccactg attgccaata
     8581 aaaatattct ggttggcatt accatcggag gatgacccgt ataagccacc ggagcaaact
     8641 tcaatcacac tgttagccgg taaagccagg ttgactttat taccgttgaa atacaatact
     8701 cctcctgagt taacgttcag ttcagatgtt ttcatgtaaa atgtatcatt accgctacca
     8761 ccgcttgagc cttgtaatac taatatagca tcgctttgga ctgtgataat tccaacagga
     8821 taaggggtag tatatgctgt gaaaggatta ttattgttaa tcccgattac agtcagctgt
     8881 atgggtgtaa gtgttgaagg ataaataaaa ttagcataaa tcgtgtttcc ggatctaatg
     8941 ttgatggcac ctgtgcccgt gtattggccc ggatatccct gagctgcggt aggggttacc
     9001 cagctggttc cgtcataacg ctgccaggtg tt
//
LOCUS       DAOPYB010000124         9007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9162_length_9007_cov_8.804290, whole genome shotgun sequence.
ACCESSION   DAOPYB010000124 DAOPYB010000000
VERSION     DAOPYB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9007
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9162_length_9007_cov_8.804290"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..3339
                     /locus_tag="PKH58_09440"
     CDS             1..3339
                     /locus_tag="PKH58_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014299558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX89295.1"
                     /translation="MTVTGTVSDTNSEPVFGAAVTVKDMTNKGTVTNIEGKFVLTNVP
                     SNGTLHISQIGMLPQDIAIGGKTAFNIILQEDTKTLGEVVVTALGVPKQARSVGYATS
                     KIAPTEIERTNAINPVTALQGKVAGVNINIGGASGVTSSSSITIRGAKSIDKNNSPIF
                     VVDGMIIQEPITGALSGTDWGSQLKNLNPADYESVTVLKGAAATALYGSRGANGAVVI
                     VSKGGKFGKKGLGIEASQTLETTDIYKSPIEFQNIYGAGSPNNGYEGGFLADGTLQKT
                     ANSFGPKMDGSILNQYLPNGESTPYVAHPDNWKTLYQSGLNSTTNVAINGGGEKSSFR
                     VSYSYTDNNGVFKRNEFKRHSISFKGLTELNKIFSLEAGVNYAFSSAQNGANQGGWNW
                     GGNVAMMSTYYSPRNMDIAAYESMYRDPLTQAVETTTPWGTLRGYLHSRDMNLNQRNE
                     NSLLSNMTLRANIAPFLTASLKGNYNYYGISTLEKQYGTGANYGPSGTGYYGRGGSIS
                     GSYNFLGMLQTTGNKINIAGETITLDAIVAAELYGNTESHSWSKGTNGGLVTPGVFAF
                     SNSKNKIEPTFNYTPRNNQTFGVSGIINLAWREQVYLELTGRNDWLSTLTYPSYMVTG
                     QNNYTVFYPSANLSWVFSETFKAPQWFTLGKLRASLARVGMGTSAYATTNGFGVFSQS
                     SQYDPTRNSVLIANPNLGTAWNNDLKPEIQQSIEIGTDLRFFNERLTLDVAYYKTNTK
                     NQIMSIPAVTESGASTQLINAGNIQNQGVEIQFEATPIRTRDFRWTIGSNFTLNRGKI
                     IKLHENVKEWQLMGAYDGGPEIWAYEGGDFGVITTPYNSSYGAAIYRFNNEENANDPR
                     NGKPVITYWGNYGSPNSVAAYGYVTNIDKGIKDRVVIGKVEPDFLLSFSTSFSYKNFD
                     FYGLIDGRVGGQYFSNTYKYASSTGVLKSSLYGRDKEHGGLPRTNYKGETVYDAIPLD
                     AVFDEGTEAPKASNPKETIDVSGLTYAEALEKGIQPMMASTYYWGNLGWGMPAELGLQ
                     ENTWFCFREMTIGYRLPESACGKFGANYLRLGVTARNLGYLLNKLTDGLNPAGISSNN
                     PLTPTDIGGVPFARTYAVNLTIKF"
     gene            3360..5201
                     /locus_tag="PKH58_09445"
     CDS             3360..5201
                     /locus_tag="PKH58_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX89296.1"
                     /translation="MNKIKYILIGAFTLLTLTGCLDEYKELNTNPELLGATDPRNAFT
                     GATENWNNSSRNHLMAKYSGVMQYMQYIVYYGGAQEGTYVNTSKSTRPSPFTPYYSDY
                     FGQIGLKLRYLVNTVIPLNAEKDRFQNVAAIANILETYEAWLMFDVNGAAPYTEAFKL
                     ATEGIRKPKYDLYQKDINGKALYKVFDEKIKENVALLQSAQANQYSLDKNDYFYGGDV
                     TKWIKFGNTLRIKMAQRLEKADKPFYTSVLNDVLSNQIGIISNNNESCIYNHPNEYNN
                     NTDDMHILTYQYCASRALVNFLTAYNDPRLPLLVRRNGFGTGNNNVSNDDVAALLAKY
                     YPNYQTQFAQWTGRYVGMSANPDSANSAWSTDSYFTLPYKDDKGVDKTMTVRHNSQIE
                     SRYYVKNGGKVGTQITARDKEDAIYDASQDAISLFTPLITYPEVCFMMAEIALKEGSA
                     KGGKDALTWFRDGIRASMEQYQAWAVKMKVPSAMNPNSDNYHPITTAAIDTYLALPEF
                     QSVSLEKIVTQQWVNLFMRPEEAWATWKRTGLPAVKAQPIPVNGVAFFEAITVSGTNM
                     LIPRRAALGAPNTENLENYNAALKDLIADPNYGPAVDKTEGRIWWDK"
     gene            complement(5385..7388)
                     /gene="uvrB"
                     /locus_tag="PKH58_09450"
     CDS             complement(5385..7388)
                     /gene="uvrB"
                     /locus_tag="PKH58_09450"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNX89297.1"
                     /translation="MDFKIVSPYRPTGDQPEAIKQLVEGINQGVPYQTLLGVTGSGKT
                     FTVANVVEQVQKPTLVLSHNKTLAAQLYSEFKNFFPQNAIEYFVSYYDYYQPEAYLPV
                     TDVYIEKDLSINQEIEKLRLATTSALLSGRRDVLVVSSVSCLYGIGNPDDFTSNVIRV
                     YKGQKIVRNAFLRSMVDSLYTRNEIELNRGNFRVKGDTVDIFLAYTDFILRVIFWGDE
                     IEEILTVEPIDYRVIESFDEYNIYPASIFVTTKDRIMSAVEMIEADLAVQVDFFRSVG
                     KDYEAKRIYERVTYDLEMIKELGYCSGIENYSRYFDGRPPGSRPFCLLDYFPKDFLLV
                     IDESHVTLPQVHAMFGGDAARKANLIEYGFRLPAARDNRPLKFEEFEKITPQTIYVSA
                     TPADYELAKSQGIVVDQLIRPTGLLDPIIDVRPSLNQIDDLLEEITLRAEADERVLVT
                     TLTKRMAEELTDYLLKMGVRCNYIHSDVDTLDRVQIMEDLRRGLYDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNHRSLTQTAGRAARNLNGKVIMYADKITASMQKTIDET
                     NRRREKQLAYNAKHGIIPTQITKGERNSLSKMEPNAYIEPEAPSILAADPVVKYMSKS
                     ALEKAIAKAKKSMQEAAKKLEFLEAAQYRDEMMKLEEMLKGKE"
     gene            complement(7396..8253)
                     /locus_tag="PKH58_09455"
     CDS             complement(7396..8253)
                     /locus_tag="PKH58_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNX89298.1"
                     /translation="MYWSNYHSHSVFCDGRSPMEEFVRFAIVRGLRRYGFSAHAPLPF
                     PTAWNMNLDDMPYYISEFYRLKEKYCSEIDLFLGLEVDFINEVFDANHNLYDTKEFDY
                     KISSVHFLDPLPAGGFFTVDGKYYDFEQGVKQLYEGDIRTVADRFFDISTLMIEKGGF
                     DIVGHVDKISLNGSKYPGFSIYEKWYQNRVGEILQLIKEKGYILEINTKSFSGEGFTY
                     PDVYFFELVNELDIPIVVGSDCHYPYLVDDGFKQVFRLLKNAGFKTMQQLTVNGWETA
                     EFNDRGFLA"
     gene            complement(8258..8476)
                     /locus_tag="PKH58_09460"
     CDS             complement(8258..8476)
                     /locus_tag="PKH58_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNX89299.1"
                     /translation="METQKKKIRRSENRMLAGVCSGIAEYFGVDTKIIRIIYFSFTII
                     TAAFPGIILYLALMLLMPREGTLPTNED"
     gene            complement(8503..8697)
                     /locus_tag="PKH58_09465"
     CDS             complement(8503..8697)
                     /locus_tag="PKH58_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNX89300.1"
                     /translation="MEEKKKLVRSKNTMIAGVCAGIAEYFGFDVTLTRIVYVLVSIFS
                     AAFPGIIVYLILMLIMPKEN"
BASE COUNT         2806 a         1828 c         1804 g         2569 t
ORIGIN      
        1 atcactgtca ccggtaccgt gtctgacaca aacagcgaac cagtttttgg agctgctgtt
       61 acagttaaag acatgaccaa caagggaaca gtgaccaata ttgaaggtaa atttgtacta
      121 acaaatgtac cttcaaatgg aactcttcac atcagccaaa tcggcatgct accacaggac
      181 attgctattg gtggcaaaac cgcatttaac attattctac aagaagacac taaaactctt
      241 ggagaagttg tggttactgc tcttggagtt cccaaacaag ctcgttcggt tgggtatgcc
      301 acctcgaaaa ttgctccgac tgaaattgaa cgcaccaatg ctatcaatcc tgtaacagca
      361 ttacagggaa aagttgcggg tgttaatatc aatattggag gtgcatctgg cgttacttca
      421 agttcatcaa ttaccattcg tggtgcaaaa tcaatcgaca aaaacaactc tcccattttt
      481 gtggttgatg gtatgatcat tcaggaacct atcaccggag ctctgtcagg tactgactgg
      541 ggttcacagt tgaagaatct taatccagcc gattatgaaa gtgttactgt tcttaaaggc
      601 gcagctgcaa ctgcacttta tggttcacgt ggtgctaacg gtgcggttgt tatagtttca
      661 aaaggtggta aattcgggaa gaaagggttg ggtattgaag cttctcaaac tctggaaact
      721 actgacattt acaaatctcc cattgaattt cagaacattt acggagccgg ttcacctaat
      781 aatggatatg aaggtggatt tttggccgat ggaactctgc aaaaaacagc aaatagtttc
      841 ggtccgaaaa tggacggatc tattttgaat caatatttac ccaatggaga atccacaccc
      901 tatgtagctc accctgataa ctggaaaacg ttgtatcaat caggattaaa tagtactaca
      961 aatgtggcta taaatggcgg cggagaaaaa tcttctttcc gtgtttcgta ctcatataca
     1021 gacaataacg gcgttttcaa acgtaatgaa ttcaaacgtc attcaatatc ttttaaagga
     1081 ttaacagaac tgaacaagat tttctcactt gaagccggag taaattatgc cttttcttct
     1141 gcacaaaacg gtgcaaatca gggaggatgg aactggggag gcaacgtagc catgatgagc
     1201 acatattata gtccccgaaa catggatatt gccgcatacg aaagtatgta tcgggatcca
     1261 ttgacacaag ctgttgaaac tactacccca tggggtacac tacgtggtta tctgcattcg
     1321 agagatatga atttgaatca acgcaatgaa aattctttat tatcgaacat gacgctgcgt
     1381 gcaaatatcg ctccattttt aacagcaagc ctaaagggta actacaatta ttacggtata
     1441 tcaacactgg aaaaacaata cggtacaggt gccaactacg gaccatctgg tacaggatat
     1501 tatggtcgag gaggtagtat atccggttcg tacaatttcc tgggtatgtt gcaaacaact
     1561 ggaaataaaa taaatattgc cggagaaacc atcacacttg atgcaattgt agctgctgaa
     1621 ctttacggaa atacagaatc tcactcatgg agtaaaggta caaatggtgg attagttaca
     1681 cccggtgttt ttgcattttc caattcgaaa aacaaaatag aaccaacatt taactataca
     1741 cctcgtaata atcaaacctt tggggtttcc ggaattatta atttagcttg gagagaacag
     1801 gtttaccttg aactgacagg acgtaatgac tggttgtcga cattgactta cccatcctat
     1861 atggttaccg gtcaaaataa ttataccgta ttttatcctt cggctaattt atcttgggtg
     1921 ttttccgaaa catttaaggc tccacaatgg tttactttag gtaaacttcg cgcttcactt
     1981 gcccgtgtag gtatgggaac aagtgcttat gccactacca atggctttgg ggtattctca
     2041 cagagttcgc aatatgatcc tacccgcaat agtgttctta ttgccaatcc aaatcttggt
     2101 actgcctgga ataatgacct aaaaccggag atccaacaat ccatcgagat cggtactgat
     2161 ttgcgctttt tcaacgaacg tttgactttg gacgttgctt actataaaac caatactaaa
     2221 aaccaaatca tgtcaatacc tgctgtaaca gaatcaggtg ccagcactca actgatcaat
     2281 gcaggtaata tccagaatca aggtgttgaa attcaatttg aagctacacc aattcgtacc
     2341 cgcgattttc gttggacgat aggttcaaac ttcacactta accgtggtaa aatcattaaa
     2401 ctgcacgaaa acgtaaaaga atggcagttg atgggagctt atgatggcgg tcctgaaatc
     2461 tgggcctatg aaggaggaga tttcggtgtt atcactactc cttacaattc atcatatgga
     2521 gccgcaattt atcgttttaa taacgaagaa aatgcaaatg atcctagaaa cggaaaacct
     2581 gttattactt actggggtaa ctatggtagt ccaaactctg tagctgcata cggatatgtg
     2641 acaaacatag ataaaggaat aaaggacaga gttgttattg gtaaagttga accggacttc
     2701 ttattaagtt tctccacctc gttctcctat aaaaactttg atttttatgg tttgattgat
     2761 ggtcgtgtag gtggtcagta cttttctaat acatacaagt acgcatcaag tacaggtgtt
     2821 ttaaaatctt ctctttatgg tcgtgataaa gaacatggag gtctgccccg taccaactat
     2881 aaaggtgaaa cagtttacga tgcaatacct cttgacgcgg tattcgatga aggaactgaa
     2941 gcccccaaag caagcaatcc caaagaaaca attgatgtta gtggattgac ttatgccgaa
     3001 gctcttgaaa aaggtattca accaatgatg gcaagcactt attactgggg taatctggga
     3061 tggggaatgc ctgctgaatt aggattacaa gaaaatacat ggttctgttt cagagaaatg
     3121 acaattggat atcgtttgcc ggaaagcgca tgcggaaaat ttggagcaaa ctacctcaga
     3181 ttaggagtga ccgcccgcaa cctgggttat ttactgaata aattaacgga cggattaaac
     3241 cctgcaggta tttcatccaa caatccatta actccgactg acatcggtgg cgttccattt
     3301 gcaagaactt atgcagttaa tttaacaatc aaattctaaa ttatataata atatacatca
     3361 tgaataagat aaaatatata ttaatcggtg cttttaccct ccttacatta accggatgtt
     3421 tggatgagta taaagaactt aatacaaacc ctgaattatt aggtgcaaca gatccacgca
     3481 atgcatttac aggagctact gaaaactgga ataacagttc acgtaatcat ttaatggcaa
     3541 aatacagcgg agtgatgcaa tatatgcaat acattgtata ctatggaggc gctcaggaag
     3601 gtacatatgt caacacttca aaatcaacac gcccttcacc atttacacca tattatagtg
     3661 attattttgg acaaatcgga ttaaaattgc gctatcttgt aaatacggtt attccattaa
     3721 atgcagaaaa agaccgtttt cagaatgtgg cggctatagc taatattctt gaaacctatg
     3781 aagcatggct gatgtttgat gtgaacggtg ccgcacccta tactgaagca tttaaacttg
     3841 caaccgaagg tattcgtaaa ccaaaatacg acttatatca gaaagatata aatgggaagg
     3901 cactttataa agtttttgat gaaaaaatca aagaaaatgt ggctttatta caaagcgctc
     3961 aggctaatca atacagtctg gataaaaacg actatttcta tggtggtgat gtaaccaaat
     4021 ggattaaatt tggcaatacc ttacgtatca aaatggcgca acggttagaa aaagccgaca
     4081 aaccttttta cacctcggtg ttgaatgatg ttctttccaa tcagatcgga attatttcaa
     4141 ataataacga atcctgtatc tacaatcatc caaatgaata caacaataat acggatgata
     4201 tgcatatcct gacatatcag tactgtgcgt cccgtgcttt agtaaatttc cttaccgctt
     4261 acaatgaccc acgactgcca ttgcttgttc gcaggaatgg atttggtaca ggtaacaaca
     4321 atgtttcaaa tgatgatgtt gctgcactct tagctaaata ctatccaaac tatcagacac
     4381 agttcgctca gtggaccgga cgttatgttg gaatgtcagc taatcctgac agtgcaaaca
     4441 gtgcgtggtc aacagattca tactttacat taccttataa agatgataaa ggagttgata
     4501 agacaatgac cgtacgtcat aatagtcaga tagaaagtcg ttactatgta aagaacggag
     4561 gtaaagtagg tactcaaatc actgcacgcg ataaagaaga tgccatttat gatgcatcac
     4621 aggatgctat cagtttattc actccactga ttacctatcc tgaggtttgt tttatgatgg
     4681 cagaaattgc attaaaggaa ggcagtgcaa aaggaggtaa ggatgctctg acatggttcc
     4741 gtgatggcat tcgtgcttca atggaacagt atcaggcctg ggctgtgaaa atgaaagtac
     4801 cttctgcaat gaatccaaat agtgataact accatccaat tactacggca gcaatcgaca
     4861 cttatctggc acttcctgaa tttcagtctg tatcgcttga aaagattgtt acacagcaat
     4921 gggtaaactt atttatgcgt ccggaagaag cctgggcaac atggaaacgc actggacttc
     4981 ctgctgttaa agcacaaccg ataccggtaa atggcgtagc attcttcgaa gcaatcacag
     5041 ttagtggtac taatatgcta ataccgcgac gtgctgcctt gggggcacca aacactgaaa
     5101 atctggaaaa ctacaatgct gcactaaaag accttattgc agatcctaat tacggtccgg
     5161 cggttgataa aacagaagga cgtatctggt gggataaata acaatctgca ttctgtttaa
     5221 ttcagaaaca cataaaagga ggtgaaatta ttttttcacc tccttttatg tgtttactta
     5281 tgttatagcc gaatatttta aaaaagggaa caataaagaa atgaagaaac taaatcaaag
     5341 agatatatcg taaagcaaca attattttct gcaattaaaa atatttattc tttccctttc
     5401 agcatttctt cgagtttcat catttcgtcg cgatactgag cagcttcgag gaattcaagc
     5461 ttcttggctg cttcctgcat cgattttttg gctttggcaa tagctttttc gagcgcggat
     5521 ttggacatat acttcactac tggatcggct gcaagaatac tgggtgcttc cggttcgatg
     5581 tatgcattcg gttccatttt gctgagtgaa tttcgctcac ctttggtaat ctgggttggt
     5641 ataatgccgt gtttggcgtt gtatgcaagt tgcttttcac gcctccggtt tgtttcatcg
     5701 attgttttct gcatgcttgc ggtaatttta tcggcataca tgattacttt cccgtttaga
     5761 ttacgagcgg ctcgtcctgc tgtctgtgtg agcgaacggt gattgcgcag aaaaccttcc
     5821 ttatcggcat ccagaatagc tacaagtgaa acttcgggta agtccagtcc ttctctcagc
     5881 aaattgacgc ctaccagaac atcgtataat ccacgccgta aatcttccat aatctgaacc
     5941 cggtcaagag tatcgacatc ggaatgaatg taattgcacc tgacacccat tttcagcaga
     6001 tagtcggtga gttcttcagc catacgcttt gtaagggtag ttacaagaac ccgttcgtcg
     6061 gcttcagcac gcaaagtgat ttcttcgagc aggtcgtcaa tctgattaag actcggacgc
     6121 acatctatga tgggatcgag caatccagtg ggccgaataa gctgatcaac cactattccc
     6181 tgacttttgg caagttcata atcggcaggt gtagcgctca catagatggt ctggggagta
     6241 attttttcaa actcttcaaa cttcagcgga cggttatctc tcgcggcagg cagacggaaa
     6301 ccatattcaa tcagattggc tttgcgggca gcatcaccgc caaacatggc gtgaacctgc
     6361 ggtaaagtaa catgactctc gtcaattacc agcaaaaagt cttttggaaa ataatcgagc
     6421 agacagaaag gtcggcttcc gggaggacga ccatcgaaat agcgtgaata attttcaatt
     6481 cctgaacaat aaccgagttc cttaatcatt tccagatcgt aagtaacccg ttcataaatc
     6541 cgtttggctt cataatcctt acctaccgaa cggaaaaaat cgacctgaac agccagatcg
     6601 gcttctatca tctcaacagc actcattatt ctatctttgg tagtgacaaa aatactggcc
     6661 ggatagatgt tatattcatc aaaactttca atcacccgat aatcgatagg ttcaaccgtt
     6721 agaatttctt caatttcatc gccccaaaag ataactctga gaataaaatc ggtatatgcc
     6781 agaaaaatat ccaccgtatc gccttttacc cggaaatttc cacggttaag ttcaatttcg
     6841 ttacgggtat acaaactatc gaccattgaa cgcagaaagg catttctgac tatcttttgt
     6901 cctttgtaaa cacgaataac attacttgta aagtcatcgg gattaccgat accgtacaga
     6961 caggaaacag aagagactac cagtacatcg cggcgacccg acaagagagc agaagtagtt
     7021 gccagcctga gcttttctat ttcctgatta atggaaaggt ctttctcaat ataaacgtca
     7081 gttaccggaa gataagcttc tggttggtaa taatcataat acgacacaaa atattcaatg
     7141 gcattctggg gaaagaaatt tttaaattcg ctgtaaagtt gtgctgccag tgttttgttg
     7201 tggctcagca ccagggttgg tttttgaacc tgctcgacaa cgttggcaac tgtaaatgtt
     7261 tttccggaac cggtcacgcc cagcagggtc tgatacggca caccctgatt aattccttcc
     7321 accagttgtt ttatggcttc gggctgatcg ccggtcggac gataaggaga aacaatttta
     7381 aaatccatcg gttttttatg ctaaaaatcc tctatcgtta aattctgcag tttcccaacc
     7441 attaacagtt aattgttgca tcgttttgaa accggcattt ttcaggagtc tgaatacctg
     7501 cttaaatcca tcatcgacaa ggtagggata atggcaatct gaacccacca caatgggtat
     7561 atccagctca tttaccaact caaaaaaata aacatcggga tacgtaaatc cttctcctga
     7621 aaatgatttt gtatttattt caagtatata tcctttctct ttaattagtt ggagtatctc
     7681 ccccactctg ttttgatacc atttttcata aatgctgaaa cccggatatt tcgagccatt
     7741 gagtgaaatt ttatcaacat gaccgacaat gtcaaaacct cctttttcaa tcatcagcgt
     7801 actgatgtcg aaaaaccggt cggcaacagt ccggatatca ccttcgtaaa gttgttttac
     7861 tccctgttcg aaatcataat acttgccatc aacagtgaaa aaacctcctg caggcaacgg
     7921 atccaaaaaa tgaacagaac ttatcttgta atcgaactct tttgtgtcgt aaagattgtg
     7981 atttgcatcg aatacttcgt tgataaaatc aacctccagg ccaagaaaca aatcaatttc
     8041 tgaacagtat ttttctttta aacgataaaa ttcagaaatg taataaggca tatcgtccag
     8101 attcatgttc caagctgtgg ggaatggaag cggagcatgc gccgagaagc catatcttct
     8161 caatcctctg acaatggcaa accggacgaa ttcctccatc ggactgcgcc catcacagaa
     8221 aacgctatgg ctatgatagt tagaccagta cacgcgttca atcttcgttc gttggcaatg
     8281 ttccttcccg gggcattagc aacatgagtg ccaggtatag aattattccc ggaaaagcag
     8341 cagtaataat ggtaaaactg aaatatatga tgcgtattat tttagtatcg acaccaaaat
     8401 actcagcgat accggagcag actcctgcaa gcattctgtt ttcagagcgt ctgatttttt
     8461 tcttctgggt ttccatagcg ttgatttcag gtgagttgat aatcagtttt ctttgggcat
     8521 aatgagcatc agaatcagat acacgataat acccggaaat gctgccgaga aaatggaaac
     8581 caaaacatat acaattcggg tcagagtaac gtcaaatcca aaatactcag ctattccggc
     8641 acacacaccg gctatcattg tgtttttgga acgtaccagt tttttctttt cttccatact
     8701 tctattgagt tattacattc acaaagataa gatattttca aaagaaaata atcttttgat
     8761 ctataaaaat aattgtctgg gaaaacgttt gtcctcccct tctgactgaa aaatataaac
     8821 cgggaatcat gagattctcg accgtcgtaa agtttagtcg atcagaaaac atgggatttg
     8881 cgaccgtcgt aaagtttaga cgaacagaaa acatgcgatt cgcgaccgtc gtaaagttta
     8941 gatgaacaga aaacatggga tttgcgaccg tcgtaaagtt tagatgaaca gaaaacatgg
     9001 gattcgc
//
LOCUS       DAOPYB010000125         8995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9183_length_8995_cov_6.399553, whole genome shotgun sequence.
ACCESSION   DAOPYB010000125 DAOPYB010000000
VERSION     DAOPYB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9183_length_8995_cov_6.399553"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(469..3822)
                     /locus_tag="PKH58_09470"
     CDS             complement(469..3822)
                     /locus_tag="PKH58_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HNX89301.1"
                     /translation="MKNKIITAITCLLISVLQLAYSQSVYTINEGKHRQERYLRYTPD
                     GRDFVIVNGKNKFNRALYGGNTGFRLETGDVPEFAFYLPLMGGNLTFSITKGTKTIQL
                     NEAEKIESRYRPGKRMYIISDPVLGKGKITIEALALYETEGAVWKITKENVSGNILLQ
                     WRFGGASFTRFSREGDMGVDPPDGFELKPQYCKGNIFKIEKNSFHLTFGPGNKENLFG
                     VFPDNSKLSTDELPALNGSVKLKEEQYIYIGRKNDISQNGLKTRFEGAETTRSELASR
                     IVINTPDPYLNTIGGALAVAADAIWDGKVWLHGAVGWRMQLTGWRAAYTGDCLGWHDR
                     ARTHFDNYAASQVTNVPLIVPHPAQDSVLNLARSEKKWGTPQYSNGYICRNPNRADQM
                     HHYDMNLCYIDELLWHLNWTGDLDYVRKVWPVITRHLAWEKLNYDPDNDGLYDAYACI
                     WASDALYYNSGAVTHATAYNYRANRMAALIAEKIGENPFPYLQETEKIGKALETTLWL
                     SSKGHWAEYKDFMGLKRLHESAAVWTVYHAIDSEVGDEFQWYQATQYIDREIPHIPVK
                     AKGLEDEGYQTISTTNWLPYSWSINNVAFAEVMHTSLAYFQAGRYENGFQLLKSSILD
                     GMYLGGSPGNFGQISFYDAARGESYRDFGDPIGVASRVFVQGLYGITPDAMNRKLVIK
                     PGFPRNWNFAQLTTPDVSFDFKRTMNEGALDTYLIKSNLENIDSLELQLPAPSDRIVG
                     ITANGKKTAWKIKPKSIDQPVIIIRLPIKKGIFVELKIDWSGEKINTDNSAKKTFRTG
                     DELSFESRNQIVDFYDPQRVLENPSIVDSKRLKGQIVGKHEFHTIFIQLKSGEMTWWH
                     PVEIKMEKERKADTRNFAHVISGKCEPVDMNQLFNSSVTDIFKNQYLSPRSPYTTLQL
                     PVQGIGEWCHPKMTAVIDDSGLRKIIRDNLLKTPLGVNFRTPEAGKNIAFTSRWDNYP
                     DQLDIPLSGYATHAYLLMAGSTNHMQSRIENGSVTVYYKDGSLDNMDLVNPDNWCPIE
                     QDYYEDDLAFEIEGERPYRLQLNSGLVSNNLERDLKIKGVYGRPIEGGAGVLLDMRLN
                     PGKELSHITLRTLSNDVVIGIMAVTLQRP"
     gene            complement(3841..5949)
                     /locus_tag="PKH58_09475"
     CDS             complement(3841..5949)
                     /locus_tag="PKH58_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89302.1"
                     /translation="MKKQLFLLTLISLISCPVSGKSGIDRKDLVSRNNPHLTTADTLG
                     ALSVGNGEFAFTADITGLQTFPTEYSKGIPLGTQSQWGWHSFPNVNGYNHNETLKEFD
                     FGRGRKELYAVQFSEKGRQQEAADYFRANPHRLHLGSVGLELKDASLSDIKNTTQTLD
                     MWNGVIFSNFEVNNSSYRVVTAAHPDKDMIAARIKTKGTNPAVKFHFPYPTGMHTDDA
                     CNWNAPDKHTSVIIQQTAYSAIIKRELDSTVYYVNIQWENKAQLIEKTKNYFVLQSDD
                     NKLNFTCQFTSQQPQTNNPTTAQTFTDARKYRNNFWKNGASVDFSACTDPRAKELERR
                     VVLSQYLLAIQCSGSTPPQETGLIYNSWYGKFHLEMIWWHQVQFALWNRPEMLEKTLR
                     WYKTAEPVAREIAKRQGFEGVRWMKMTDPTASEAPSGVGSFLIWQQPHFIYMAELIYR
                     ARKNPETINKYYDLVQKTAEFMYSFATYDKEKDRFVLKGIIPAQETLRASETVNPPFE
                     LSYWHFVLELAQQWRERNGEKRKAEWDNLINKLSPLAFNEDHLYLAAETAPDTYSDIR
                     FTSDHMAVLGALGMLPANKLIRQEYMKNTLNWIWDNWNWNKTWGWDYPMTAMNAARLG
                     EPEKAVGALLMDKRTNTYLVNGHNYQDARLRVYLPGNGGLLTAVAMMCAGWDGSKGEN
                     PGFPKDGKWNVKWEGLQKMP"
     gene            complement(6332..8878)
                     /locus_tag="PKH58_09480"
     CDS             complement(6332..8878)
                     /locus_tag="PKH58_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HNX89303.1"
                     /translation="MTTRRLFLNILLLFSLHAFPASGFVWFDGKQPVSYSVTASKPVV
                     SVALNMFHDDLKQVTGTVPQQLSEKNAKIAIIQLDDKNLSAAQKQILQRKGIPVNDLS
                     SKKDAFFMAVIGRGAGAKLYIVGSDGRGTAYGILELSRKAGVSPWVWWSDVTPEKQNK
                     LTLPAGFSTFQSPSVEFRGIFLNDEDWSMQPWSWMNFEPSDIKGKIGARTYKEIFKLL
                     MRLRANAIWPGMHGTTTPFYFVPGAKEAADSCGIVIGTSHCEPLMRNNVGEWDENKRG
                     RYNYITNKQSVTDYWVERLREVDDKENVYTIGMRGVHDGAMEGVKTMKEQTEALQRVI
                     YDQRNLLKQYVDPNVEQIPQQFVPYKEVLQIYENGLDVPDDVMLTWCDDNYGYMTRLS
                     DAQQQKRSGGAGVYYHLSYWGRPHDYLWLTTTQPGLIYNEMREAYNHNARRLWIVNVH
                     DLKPAAYDLEFFLDMAWDINSITPSTIRDHQAEWLKREFGDLAGKKLLPAMMEFYRLC
                     GIRKPEFMGWTQVELDKKLYARGWSPLKDTEFSLSEFGGELDRYLEAYENIKQTVADV
                     EKIIPANRKDAFFSHIKYPVFSAAAMSTKMLEAQRARAIASGSYDFGRWERDHELMVA
                     CAKSMNAYQEIRRLTDYYNNEMAGGKWKHSMVYNPRDLYVFYAPLLPMAPTDAEIEKF
                     SSGVNPKSKSLTDIKNDSFIAANACNFSKATAGATPVQSLGHSMNAVSLPKGGSLTYE
                     FESPWEGEAVLRTAVIPTQPNDKGDIRFSVSIDGGQPQICSYKEKFRSDAWKENVMRG
                     QAIRNTTHTLAKGKHTLVITALDNHVVVDQWMLDFKPQRSFYLFPLDASY"
BASE COUNT         2267 a         2068 c         1961 g         2699 t
ORIGIN      
        1 atttatttat cggcccccta cccccctaaa gggggaatat tgagcgtata tcatttgttg
       61 tgagtttttt gatcacgcca gtgtgtgtca aaaatatttt acttggacat gtgtgtttca
      121 tgtgtgagta tgtttgattt attataacag taaaaatatt tcaccctgtt atttatgcgt
      181 tctgaatggg atagcgggta atcctgcctt gttgcacaaa tctggcattg cccgttcatt
      241 cgacaaaaaa cgaacagcaa caggtttatg tatcataagt ggtgccaaaa atagctttta
      301 aaaaaacggt attgattaca aaaatgattt ttaaaatgct ttatttgata aaatccaaaa
      361 ataaaactgt cctgtcattc attttgcgta cttcatgtga ttgtttaccc ttcattcagt
      421 gaagcagttc taccgccatt cgtcataaag attgctctgt ttcacaaact aaggcctttg
      481 caaagtaaca gccatgatac cgataacaac atcattcgac aaagttctca gtgttatgtg
      541 actcaattcc tttccgggat tcaaccgcat gtcaagcagt acaccagccc cgccctctat
      601 tggtcggcca tatactcctt tgattttcaa atctctttcg aggttattcg aaacaagtcc
      661 ggaattaagt tgtaaacgat aagggcgttc accttcgatt tcaaatgcca gatcgtcttc
      721 ataataatcc tgctcaatgg ggcaccagtt atccggattt accagatcca tgttatccag
      781 gcttccatcc ttgtaataca ccgttactga gccattttcg atgcggctct gcatgtggtt
      841 ggtgctgcct gccatcagca aatacgcatg ggttgcatat cccgaaaggg gaatatccaa
      901 ctgatcgggg taattgtccc aacgtgatgt gaaagcgatg tttttccctg cttcaggagt
      961 tcggaaattg actccgagtg gtgtcttgag caaattatcc cgaatgattt ttctcaaccc
     1021 ggaatcatcg attactgcgg tcattttcgg atggcaccat tctccgattc cttgcacagg
     1081 cagttgcagg gtggtgtaag gtgaccttgg cgacaggtac tggttcttga aaatgtccgt
     1141 taccgatgaa ttgaataatt gattcatatc taccggttcg cacttgccgg aaatgacatg
     1201 agcaaaattc cgagtatcag ctttccgttc cttttccatt tttatttcga ctggatgcca
     1261 ccaggtcatt tcgcccgatt tcagttggat aaaaattgta tgaaactcat gcttaccaac
     1321 gatctgtcct ttcaaccgtt tagaatcaac aatcgaagga ttttcaagca cccgctgagg
     1381 gtcataaaaa tccactattt ggttccgact ctcgaaagac agttcatcac ctgtccgaaa
     1441 tgttttttta gccgaattat cggtgtttat tttttctccg ctccaatcaa tcttcaattc
     1501 aacgaatatc ccttttttga tgggtaaccg gatgatgata accggctggt cgattgattt
     1561 cggttttatt ttccatgctg ttttttttcc attagccgtg ataccaacaa tacggtcaga
     1621 cggagcagga agctgcaatt ccagcgaatc gatgttttcc agattgcttt taataaggta
     1681 agtatcgagc gcaccttcat tcatcgttct tttgaaatca aacgaaacat caggagtggt
     1741 taactgagca aaattccagt tacgcggaaa tcccggtttt ataaccaact ttctgttcat
     1801 agcatcggga gtgattccgt acagtccctg cacgaatacc cgggatgcaa ctccgatagg
     1861 gtctccgaaa tcacggtaac tttcaccccg cgctgcatcg taaaaactta tctgtccaaa
     1921 atttccggga ctacctccca ggtacatccc atccaggatg gaacttttga gcaactgaaa
     1981 tccgttttca tacctgccgg cttgaaaata ggcaagcgaa gtgtgcatca cttcagcaaa
     2041 agctacatta ttgatgctcc aggagtaagg aagccaattg gtagtggaga tggtttgata
     2101 accttcatct tcgagtcctt ttgctttcac ggggatatgc ggaatttccc ggtcgatgta
     2161 ttgcgtagcc tgataccact ggaattcatc acccacctcg ctgtcaatgg catggtacac
     2221 tgtccatacc gctgcgctct cgtgcaacct tttcagtccc atgaaatcct tgtattccgc
     2281 ccaatgacct ttggaggaca accacagagt agtttcaagc gctttcccga ttttctctgt
     2341 ttcctgcagg taagggaaag gattctcacc gattttttcg gctatcaatg ccgccattct
     2401 gttagcgcgg tagttgtaag ctgttgcgtg tgttaccgca ccgctgttgt agtagagtgc
     2461 atcactcgcc cagatgcagg cgtaagcgtc atagagtcca tcgttgtcgg ggtcgtaatt
     2521 caacttctcc caggcaaggt gtcgggtaat taccggccat acttttcgca cataatccaa
     2581 atcacccgtc caattgagat gccagagcag ttcgtcgatg tagcacaaat tcatgtcgta
     2641 gtgatgcatt tgatcggctc tgtttggatt tctgcagata tatccgttac tgtactgagg
     2701 cgtaccccat tttttttcag agcgtgcgag gttcagtaca ctatcctgag ccggatgtgg
     2761 gacgataagc ggaacatttg tcacctgact ggctgcataa ttgtcaaaat gagtgcgtgc
     2821 gcggtcgtgc caaccgagac aatcaccggt gtatgccgct ctccagcccg taagctgcat
     2881 tcgccagcct actgctccgt gaagccacac cttgccatcc caaatagcat cagcagcaac
     2941 ggccagtgct ccaccaatcg tattcaaata gggatccggt gtattaatta cgattcgtga
     3001 agccagttcg cttcgggttg tctctgcacc ttcaaatctt gtttttaacc cgttttgact
     3061 tatgtcgttt tttcttccaa tgtagatgta ttgctcctct ttaagtttaa cagagccatt
     3121 caaagccgga agttcatcgg ttgataattt cgaattatca gggaaaacac caaaaagatt
     3181 ttctttgttt cccggtccga aggtaaggtg gaagctgttc ttttcaatct taaaaatatt
     3241 ccccttacag tattgtggtt tcaattcaaa accgtcgggc ggatcaacgc ccatgtcacc
     3301 ttccctggaa aagcgggtga acgaggcacc tccaaatctc cattggagta gaatattccc
     3361 ggaaacattt tctttcgtga ttttccaaac agcaccttca gtttcgtaga gtgcaagtgc
     3421 ctcaatggtt atttttccct ttcccaaaac cgggtcagaa ataatataca ttctctttcc
     3481 gggacggtat ctgctctcaa ttttttcagc ctcgttgagt tggattgttt ttgttccttt
     3541 ggtaatggaa aatgtgagat ttcctcccat taggggaaga taaaaagcaa attcggggac
     3601 atcgcccgtt tcgagcctga agccggtatt accgccatac aatgcccgat taaatttgtt
     3661 tttcccgttg acaatgacaa aatcacggcc gtcaggcgta taccgcaaat atctttcctg
     3721 acggtgtttg ccttcgttaa tcgtgtaaac cgactgtgaa taagctaatt gcaatacgga
     3781 aataagcagg caagttattg ctgttattat cttatttttc atttcaaaat tcttagaaaa
     3841 ttaaggcatt ttctgtaatc cttcccattt cacattccat tttccatctt tcggaaatcc
     3901 gggattttct cctttgcttc catcccaacc ggcacacatc atggccacag cagtgagtaa
     3961 tccgccgttg ccggggaggt aaacccgcaa acgggcatcc tgataattgt gaccgttgac
     4021 aaggtaggta ttggttcgtt tgtccatcag cagggctcca accgcttttt cgggttcacc
     4081 cagccgcgct gcattcatgg cagtcatcgg ataatcccag ccccaggttt tgttccagtt
     4141 ccagttgtcc caaatccagt tcagcgtatt tttcatgtac tcttgcctga tgagtttgtt
     4201 agcaggcagc atacccaatg caccgagtac cgccatgtgg tctgacgtaa aacgaatatc
     4261 gctgtaggta tccggtgctg tttcggcagc caggtaaaga tggtcttcat tgaatgcaag
     4321 cggtgataat ttatttatca ggttgtccca ttccgctttc cgtttttctc cgtttcgctc
     4381 tcgccattgt tgtgccagtt ccaggacaaa gtgccagtat gaaagttcaa atggtggatt
     4441 taccgtttcg gaagcacgca gcgtttcctg agccggaata attcctttca gcacaaaacg
     4501 gtctttttct ttgtcataag tggcaaaaga atacatgaat tcagccgttt tctgcactaa
     4561 atcatagtat ttattaatcg tttcagggtt tttcctggcg cgataaatca attcagccat
     4621 gtaaataaag tgcggttgtt gccaaatgag gaaactaccc acaccggaag gagcttcaga
     4681 agcagtcgga tcggtcattt tcatccaccg aacgccctca aagccctgtc ttttggcaat
     4741 ttcacgggct acaggctcag cagttttgta ccatctcagc gttttttcaa gcatttcggg
     4801 acggttccac aatgcaaact gaacctgatg ccaccaaatc atttcgagat ggaattttcc
     4861 gtaccaggaa ttatagatca gtccggtttc ctgaggcgga gtagagccgg aacactgaat
     4921 agcaaggagg tactgactta agaccactct tctttcaagt tctttggcac ggggatcggt
     4981 acacgctgaa aaatcgacag aagcaccgtt tttccagaaa ttattccggt attttcgggc
     5041 atcagtaaaa gtctgagcgg ttgtgggatt gtttgtttgc ggttgttgcg atgtaaattg
     5101 acaagtgaaa tttagcttgt tatcgtccga ctgaagaaca aaatagtttt tggttttttc
     5161 aattaactgc gctttatttt cccattgaat gttgacataa taaaccgtac tgtccagttc
     5221 acgtttgata atggcagagt aagcggtttg ctgaatgatt acggaggtgt gtttatcggg
     5281 agcattccag ttgcatgcat catcagtatg cataccggta ggataaggaa aatgaaattt
     5341 tacagcggga tttgtacctt ttgtttttat tcgcgcagca atcatatcct tatccggatg
     5401 tgccgctgtt accactctgt aagatgaatt gttcacctca aagttactga atatcacccc
     5461 attccacata tccagggtct gtgttgtatt tttgatatcc gataatgaag catccttcag
     5521 ctcaaggcct accgagccta aatgtaagcg atgcgggttg gctctgaaat aatcggctgc
     5581 ttcctgttgc cgtccttttt cactgaactg aacggcgtaa agttcctttc taccccttcc
     5641 aaaatcaaac tctttcagcg tttcattgtg attgtatcca tttacattcg gaaaactgtg
     5701 ccagccccac tgactttgag ttcccagcgg tattcctttg ctgtactcag tcggaaaagt
     5761 ctgcaaaccc gtaatatcgg cagtaaaagc aaattcaccg ttgccaacgg aaagagcgcc
     5821 cagcgtatcg gctgtagtaa gatgtggatt atttcttgat accagatctt ttcggtcaat
     5881 tccggattta ccactcaccg ggcaggaaat aagagaaatt aaggtcaata aaaagagttg
     5941 tttcttcata tttattatct catagaaact cagatgctca atgtttaatc attttgatgt
     6001 tgcttgatga tcatttgatt gattatgatg aagtcatcct gactttttta aataactgtt
     6061 ttatgtgatc ttgacttcat agaagtcaca tatttgtaga aaaaatgtcc ctttttgatg
     6121 tacgacccca atcggggtcg aataattcaa ggtgattttt taactacaaa tatgaaattc
     6181 cgctggaatt tttctttaaa acaaaaagtc aagaagaact tgatatttaa aagagactac
     6241 gctaatttta cttcaaaatt tttcagttta caaacggaat acttgttttt gtgcgtttca
     6301 tttcctaatc atgttaaaga attttttatt ttcaatacga agcatccagg ggaaagaggt
     6361 aaaagctgcg ctgcggtttg aagtcgagca tccactggtc cacaaccaca tggttatcca
     6421 gtgcggtgat aaccagcgta tgtttccctt tggcaagcgt atgcgtggta ttgcggatgg
     6481 cttgcccgcg catcacgttt tctttccagg cgtcggagcg gaatttttcc ttatatgaac
     6541 aaatttgggg ttgccctcca tcaatactta cgctgaagcg tatatcacct ttatcgttgg
     6601 gctgggtggg tataacagca gttcgcaaaa ccgcttcacc ttcccaggga ctttcaaatt
     6661 catacgtgag cgaaccgcct ttgggcagcg acaccgcatt catgctgtgt ccgagcgatt
     6721 ggacaggagt ggcaccggcc gtagccttgc tgaaattgca ggcattggca gctatgaaac
     6781 tatcgttttt gatatccgtg agtgattttg attttggatt aacaccgctg ctgaactttt
     6841 ctatttcagc atcggtggga gccattggca gtaacggagc ataaaacaca tacaaatcgc
     6901 ggggattgta caccatggag tgtttccatt ttccaccggc catttcgttg ttgtagtaat
     6961 cggtgaggcg gcgaatttcc tgataagcgt tcatgctttt ggcacaagcc accatcagtt
     7021 cgtggtcacg ctcccatctc ccaaaatcgt aactgccgga agctatggcg cgagcgcgct
     7081 gggcttcaag cattttggtg ctcatggcag cggctgaaaa gaccggatat ttgatatgtg
     7141 aaaaaaaggc atctttccgg ttggcaggaa ttattttttc cacatcagct actgtctgtt
     7201 ttatgttttc atacgcttcg aggtagcggt ccagttctcc tccgaattcc gagaggctga
     7261 attccgtatc tttcagtggc gaccatccgc gggcatacag ttttttatcc agttccacct
     7321 gcgtccatcc cataaattcg ggtttgcgta tgccgcaaag gcgataaaat tccatcatgg
     7381 cgggaaggag ttttttaccg gctaaatcgc caaattcgcg tttcagccat tcagcctgat
     7441 ggtcacgaat ggtagagggc gtaatggaat tgatatccca ggccatatcg aggaaaaact
     7501 ccaggtcgta agcggccggt ttcagatcgt gcacattcac tatccacaag cggcgtgcat
     7561 tgtggttgta ggcttcgcgc atttcgttgt atatcagacc cggctgtgtg gtagtgagcc
     7621 acaggtaatc gtgtggacgc ccccagtagc tcaaatggta atacactccc gcgccgccgc
     7681 tccgtttttg ctgctgagca tcgctgaggc gggtcatgta tccgtagtta tcgtcacacc
     7741 aggtgagcat cacatcatca ggtacatcca gtccgttttc gtaaatctgc aggacctcct
     7801 tgtagggtac aaactgctgt ggaatttgtt cgacattcgg atctacgtac tgtttcaaca
     7861 agtttcgctg atcgtaaata acccgctgta atgcctctgt ctgctctttc atggttttca
     7921 cgccttccat tgcaccatcg tgcacaccgc gcatcccgat ggtgtacaca ttctctttat
     7981 cgtccacctc ccggagcctt tccacccaat aatcagtcac agactgctta ttggtgatgt
     8041 agttgtatct gccgcgttta ttttcgtccc attcgcccac gttattgcgc atcagcggtt
     8101 cgcagtgcga tgtaccgatg acaatgccgc aactgtcggc cgcttctttg gcacccggca
     8161 caaaataaaa cggagtagta gtgccgtgca ttcccggcca gatggcattg gcacggagac
     8221 gcatcaggag tttgaaaatt tctttgtacg tacgcgcacc gattttgcct ttgatatcag
     8281 aaggttcgaa attcatccag ctccagggct gcatactcca gtcttcatcg ttgaggaaaa
     8341 tgccccgaaa ttccacggat ggactttgga aagtgctgaa accggcagga agtgtgagtt
     8401 tgttttgttt ttcgggagta acatcgctcc accacaccca gggcgaaacg ccggctttgc
     8461 gcgagagttc gagaatgccg taagctgttc cgcggccgtc gctcccaaca atgtaaagtt
     8521 tggcgccggc acccctgccg ataacagcca taaaaaatgc atcttttttg gatgataaat
     8581 cgttgacagg aattccttta cgctgtaaga tttgtttttg tgcggccgac agatttttat
     8641 catccagctg aatgatggcg atttttgcat ttttttcaga cagctgctgc ggaacagtcc
     8701 ctgtaacctg cttcaaatcg tcgtgaaaca tattcagagc aaccgaaaca acaggtttag
     8761 aagcggtaac agaatagctg acaggttgtt ttccatcaaa ccagacaaaa ccggatgccg
     8821 ggaaagcgtg gagtgagaaa aggagaagaa tgtttaagaa taaacgacgc gttgtcatga
     8881 cagattgatt ttcaggatat agacgtttca tccctgtatc caactatatt tcggatattt
     8941 ttggggaacg aaatgagcat tctgcaggtt aatttcgttt gcaaatataa aaaac
//
LOCUS       DAOPYB010000126         8988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9193_length_8988_cov_8.756521, whole genome shotgun sequence.
ACCESSION   DAOPYB010000126 DAOPYB010000000
VERSION     DAOPYB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9193_length_8988_cov_8.756521"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKH58_09485"
     CDS             complement(<1..704)
                     /locus_tag="PKH58_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX89304.1"
                     /translation="MNQDKKNITYDALVIGAGIAGGEAALNLANTGFKVLLVDKDDSI
                     GGKMILLSKVFPTLDCAACITTPKVSEISRHPNITIFTGSKVDNIRRVNENEFTATIY
                     KKPRYVHVEDCTACQKCEQACPVSVSDQYQFGLVGRKAAYIPFSICNPKAAVIDIENC
                     TLCGACEKACPTDCIDFTMEEEQYDLHVRTVVLATGFNLFDPLKMPRYGFGTYKNVLT
                     SMQMERQIAPTRPFHT"
     gene            complement(701..1114)
                     /locus_tag="PKH58_09490"
     CDS             complement(701..1114)
                     /locus_tag="PKH58_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNX89305.1"
                     /translation="MAIATKKTAKILVFSTEKISDPAIDMAGLMKLHYPPTVYTITVP
                     CSSSIKSKWILYAYEKGFDGVFIAADGSDCVYGESCTEKTGSIVTKTHELMKSQEIEP
                     ARLRMAAICSVCSESFVKQIRSFNDYLLKTLHPES"
     gene            complement(1240..1491)
                     /locus_tag="PKH58_09495"
     CDS             complement(1240..1491)
                     /locus_tag="PKH58_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNX89306.1"
                     /translation="MTNEELKNLVVAKSVDARGTACPGPLLEAKKAIGAIKTDDIMEV
                     LSADEGTKVDIPKWCNKQGHEYLGFVEENGYFKIYLKKQ"
     gene            complement(1645..2211)
                     /locus_tag="PKH58_09500"
     CDS             complement(1645..2211)
                     /locus_tag="PKH58_09500"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNX89307.1"
                     /translation="MENTNEVTVAQLQTQLEALQKKVNKLEHSRQDQLSMAIVSGDMD
                     KILAAMIISLAAAAMDTQVKLFFSFWALAALRDPKKNPKGKDFISKMFGMMLPKSRNN
                     LKLSNMNMAGMGPVMIKSLMKKKNVMSLDEMFRQAAELGIEITVCEMSMDLMGFKKEE
                     IIDYPHLRYAGATTFVADAGESAMQLFI"
     gene            2359..3036
                     /locus_tag="PKH58_09505"
     CDS             2359..3036
                     /locus_tag="PKH58_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX89308.1"
                     /translation="MNTDCKSCPFNTRAAHTLSDSDFNQLSDNHAVVNFVKGDSIIKQ
                     GTFSTNVAFLRMGLVKLHLTGPHHEQIVRLVKAPTYLGLPTTFGDKINQYSVTAVSDC
                     SVCFIDVTIFRRLLKDNEQFSYEIILDLCRNELESFRRCANRTQRQTRGNIAAFLLEL
                     SDIIYESDEFALPLSQSEIGNLVDATRESVNRVLTEFDKDEIISLKGKNVEIINKKSL
                     QLISQNG"
     assembly_gap    3338..3437
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3646..4446
                     /locus_tag="PKH58_09510"
     CDS             3646..4446
                     /locus_tag="PKH58_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNX89309.1"
                     /translation="MKAHEIDYTIYGEEMQYIEIELDPQEVVIAEAGSFMMMDSGIQM
                     ETIFGDGSGNDTGILGKLLSAGKRVVTGESLFMTAFENRNNTRSRISFASPYPGKIIP
                     IDLNQYQGKFICQKDAFLCAAKGVSVGIEFNRKLGTGLFGGEGFIMEKLEGDGLAFVH
                     AGGTLARKELQAGEILKVDTGCIVGFTSGIQYDVEFVGGIKNTIFGGEGLFFATLRGP
                     GIVYVQSLPFSRLAQRILASVPRTGGSRREEGGLLGGLGNLLDGDNRF"
     gene            5001..6533
                     /locus_tag="PKH58_09515"
     CDS             5001..6533
                     /locus_tag="PKH58_09515"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNX89310.1"
                     /translation="MKLSNKTKSIALFTAIVIVVSAATSYATNYVSNKKKGVEFQTTS
                     DQTLSAQARFASASSAMETDFTVAADLTVHAVVYIKTKAMVSQQQQNFFNDPLFDFFF
                     GPQQGQRQQNNTPKQEVPKGAGSGVIISQDGYIVTNNHVIDDASDIEVTLNDKRTFKA
                     KLIGADPSTDLALLKIDANDLQAITIGNSDNVKVGEWVLAVGNPFNLSSTVTAGIVSA
                     KARNIGIIGQNNFGEPQSNNMSIESFIQTDAAINPGNSGGALVNTRGELIGINTAIAS
                     GNGVYNGYGFAIPSSIVSKVVTDLRQHGAVQRALLGVSIQDLSTEVAKEKNIKTLEGA
                     LVADVVSDGAADKAGIKTNDVINEINGVKVKSVAELQEQVGRYRPGDKVTVGLMRENK
                     PMKLTAQLINADGNTDMVSSSTVDSDLGATLKPVDNKIKKALGIDYGLTVESVTKGAL
                     YNQGIRQGYIILKINNQPMETMKDFKEVYNEALKKDKKLYIAGVYPTTGKVAYYEIKL
                     DK"
     gene            6627..7454
                     /locus_tag="PKH58_09520"
     CDS             6627..7454
                     /locus_tag="PKH58_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNX89311.1"
                     /translation="MKSILKLFILIISLNVSSLYASKVDTVLTYSPSMKKSIKAVVIT
                     PDTYTKTEKMPVLYLLHGYSGKYDNWTLHMPNINYLADQYQFIVVCPDGNYGSWYFDS
                     PIDSTFRYETYVAGELIQWVDKNYKTIKNRSGRAISGLSMGGHGALYLAFRHQDVFGA
                     CGSMSGGVDIRPFPNNWDLSKRLGEQRSCPQNWENNTVINQLHLLTPKSLNIIFDCGT
                     EDFFYPVNVRLHEELTYRNIPHDFISRPGQHNREYWNNAVKYQAQFFSDFFKSSEKI"
     gene            7698..8558
                     /locus_tag="PKH58_09525"
     CDS             7698..8558
                     /locus_tag="PKH58_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNX89312.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIK
                     NGDRLALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQENERKPSAEELAD
                     ELDIPVDKIADTMKVSGRHVSVDAPFVEGEDNSLLDVMINDDSPNADRVLINESLSKE
                     IERALSTLSEREHEIVKKFFGIGIPEMTLEEIGDEFGLTRERVRQIKEKAIRRLRQSS
                     KSKLLKTYLG"
BASE COUNT         2614 a         1832 c         1829 g         2613 t
ORIGIN      
        1 atggtgtgga acggacgcgt gggagctatc tggcgttcca tttgcatgga ggtaagtacg
       61 tttttgtagg ttccaaatcc gtaacgcggc atcttcaacg gatcaaagag gttgaagccc
      121 gtggcgagaa caacagtgcg tacatgcagg tcatactgtt cttcttccat ggtgaaatca
      181 atgcaatcgg taggacatgc tttttcgcag gcgccgcaga gcgtgcagtt ctcgatatcg
      241 atgacggcag ctttcggatt acagatactg aacggaatat aggcagcctt gcgccccacc
      301 agtccgaact gatactggtc actgacactt accgggcagg cttgttcgca tttctgacaa
      361 gccgtgcagt cttccacatg cacgtagcgt ggttttttat agatggttgc ggtaaattcg
      421 ttttcgttta ctcttcttat attatcaacc ttacttcctg tgaagatggt tatgttggga
      481 tgtctggata tctccgatac tttgggggtg gttatgcagg cggcacagtc gagggtggga
      541 aaaactttgc tcaacaaaat cattttccct ccgatgctgt cgtccttatc cacgagcagc
      601 actttgaaac ctgtattggc aaggttgagg gcggcttcac caccggcaat cccggctccg
      661 atgactaatg cgtcatacgt tatgtttttc ttatcctggt tcacgattct gggtgtaaag
      721 tttttaataa ataatcgttg aaacttctga tttgcttcac aaaagactcg ctgcacaccg
      781 agcagatggc tgccatacgc agacgtgccg gttcaatctc ttgtgatttc atcagttcat
      841 gtgtttttgt cactatacta ccggtttttt cggtacagct ttcgccatac acacagtcgc
      901 ttccgtcggc agcgataaat acgccgtcaa accctttttc ataagcatat aaaatccatt
      961 ttgattttat actactcgaa caaggcacag taatggtata tactgtcggt ggataatgca
     1021 gtttcatcaa tccggccatg tcaatggccg gatcagaaat cttctcggtt gagaaaacca
     1081 gtattttcgc tgtttttttt gtagcaatag ccataatgtt gtttgagttt ctgagttatg
     1141 gaaattcaga aaaataaatt tcgttgaaag aatggacaat aaaactcccg accgttggag
     1201 ccccgatact ctccaaccgg ttgggagttc attctaatct tattgttttt tcaaataaat
     1261 cttgaaatat ccgttttctt ctacaaatcc gagatattca tgtccctgtt tgttacacca
     1321 ttttggtata tccacctttg ttccttcatc ggccgaaaga acttccataa tatcgtcagt
     1381 cttgattgct ccaatagctt ttttagcttc gagaagtggt cccgggcatg ctgtaccgcg
     1441 tgcatctact gactttgcta ctactaagtt ttttaattct tcgtttgtca taattttata
     1501 gattttaaag accggctcct accccccctt aaggagaaat ttaagtgcaa aggtcaatgt
     1561 tatatgtatt tagaaaattt attttgtaaa atttgcttaa aaaatgctcc attccccgct
     1621 tcagggggtt ggtgggcagt gattttaaat aaacaactgc atggcgcttt cgccggcatc
     1681 ggccacaaag gtggtagctc ccgcatagcg caggtgtgga taatcaatga tttcttcctt
     1741 tttgaaaccc atcaggtcca ttgacatttc gcaaacggtg atttcaatgc ccagttcggc
     1801 tgcctgacga aacatttcat ccagcgacat cacgtttttc tttttcatca gtgacttaat
     1861 catcaccgga cccatgcctg ccatgttcat gttcgatagt ttcaggttgt ttctgctttt
     1921 cggcagcatc attccaaaca tttttgaaat aaaatccttg cctttcggat ttttcttcgg
     1981 atcgcgtaag gcagccagcg cccagaagga gaaaaagagc ttcacctgcg tatccatagc
     2041 tgcagctgcc agtgaaataa tcatggctgc cagaatttta tccatatcac ccgatacaat
     2101 tgccattgac agttgatcct gacgactgtg ttcgagtttg ttcacttttt tctgcaacgc
     2161 ttcaagttgg gtctgtagct gtgctactgt tacctcattg gtattttcca ttttctatta
     2221 atttatttat tttattggaa gattaattag agatttattt ctgcaaattt atattaatgt
     2281 aatatcactt ttcgtttttg ttgtgtgtta gtgcctacat tattttgtga ttcaaatcac
     2341 atctgttata tttgctgaat gaatacagat tgtaagtctt gtccttttaa caccagagca
     2401 gctcatacgt tgagtgacag tgattttaac caactatcgg ataaccatgc tgtagtaaac
     2461 tttgtaaaag gtgactctat cattaaacag ggaacttttt caacgaatgt ggcatttctc
     2521 cgtatgggtt tggtaaagtt acaccttacc gggcctcatc acgaacaaat tgtacggttg
     2581 gtgaaagctc ctacatacct tggtttacct actactttcg gcgataagat caatcagtat
     2641 tccgttacgg ccgtgtccga ttgttcagta tgttttatcg atgtaactat cttcagacgt
     2701 ttattgaaag ataatgaaca gttttcatat gaaatcatac tggatttatg ccgcaacgaa
     2761 ttggaatcat ttcgccgctg cgccaaccgt acgcaacgcc agacacgggg aaacattgct
     2821 gcttttctgc tggaattgtc ggatataatc tatgaatctg atgaatttgc gttacccctt
     2881 tctcagtcgg aaataggtaa cctggtggac gctacccgcg aaagtgtaaa ccgggtgctt
     2941 acggagtttg ataaagacga gattatcagc ctgaaaggaa agaatgtgga aattatcaat
     3001 aaaaaatcat tacagctgat tagtcagaat gggtgaaatg ttcttgtgta aggcaaaatt
     3061 gtaaattggc ttccaactca ttaagactta tttatggaca aaattatcat tttcgttgtt
     3121 ttatcagttc tgatagtaat tgtttcatgg aggactttac tgaactataa aactcatgga
     3181 ttttatagat ttatcagttg ggagtgtatt gcatggttga tatcttcaaa atataaagtc
     3241 tggtttattg ttcctttttc aatccggcag atattttcct ggatttttct tatcatatca
     3301 atatatctgg ttgttgccgg agtaatcctt ttgaaaannn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnntgt ctgacttata cacaaacctg ataaactcag caattatgat
     3481 gattttatac agaaatatag tacttttatg cgcgaaatac aagcttgcgg tctgactccg
     3541 tttcagtaaa actttgggct tctgaataca ataatttaaa gggccaaatg caggattttg
     3601 tataggaaaa acaaccaatt aaatggaata aaatatcaga aacttatgaa agcacacgaa
     3661 attgactaca cgatatacgg cgaagagatg cagtatattg aaattgaact cgatccgcaa
     3721 gaagtggtaa ttgccgaagc cggcagtttc atgatgatgg atagcggcat acagatggaa
     3781 accatttttg gcgatggttc gggtaacgac accggcattc tgggtaaact actctcagcc
     3841 ggtaaacggg tggtgacggg agaaagcctg tttatgaccg ccttcgaaaa ccggaataac
     3901 accagaagcc gcatcagttt tgcatccccc tatcccggta aaattatccc aattgatctg
     3961 aaccagtatc agggcaaatt tatttgtcag aaggacgcat tcctttgcgc tgccaagggc
     4021 gtgtcggtgg gtatcgagtt taaccgcaaa ctgggtaccg gcttgttcgg cggcgaaggc
     4081 ttcatcatgg agaaacttga aggcgacggt ttggcatttg tacacgccgg aggcacactg
     4141 gcacgcaaag aactacaagc cggcgagata ctgaaagtgg atacaggctg catcgtagga
     4201 tttacaagcg gaattcagta cgatgtggaa tttgtcggtg gcatcaaaaa cacgatattc
     4261 ggtggtgaag gtctgttctt cgcaacccta cggggacccg gcatcgtata tgtgcaatcg
     4321 ctgcctttca gccggctggc tcagcggata ctggcatcgg ttccacgcac aggtggaagc
     4381 cggcgcgaag aaggcggatt gctgggtggt ctgggcaact tgctggatgg cgacaacagg
     4441 ttctgaaaaa atacagtata aactgtttta cgggccgtat ttgtttatca gatacggctt
     4501 cgtatttttt gtggattgta tataattaaa caccgactga atatcgggca tcaaaccatt
     4561 ttcatgtgtt taaaaatata tgccacccac cctctaaagg gagaaatcgt tttctcaaat
     4621 ggctgtttct gcacttacag gctattgttt ttgctctccg gatttatttc cctgctgcat
     4681 tttagaaaaa atagtgtgca aaaatgtcag gtgctgacat ttttacacat tttccaccac
     4741 gcgcctgaat tctctttcgg aaattttgtc agctattcat tttatattta ttaaatcttg
     4801 ctattactct gcgttaaagc ccgttaaaca gcattttaac attgttaaat ttgaagaaat
     4861 atcacttcgg attgaaacat cgggcatgta gtttgtataa tttagtatct gaaaaaacaa
     4921 ataagttaca actggcagaa attctgttta tgccatcacc aacttaaaaa cagtacaaat
     4981 taataaacat aaaaaagatt atgaaactaa gtaacaaaac aaagtcaatc gcactattta
     5041 ctgctattgt aattgtggtt agtgcagcaa ccagttatgc aacaaattat gtttcgaaca
     5101 agaaaaaagg agttgagttt cagacaactt ctgatcagac gctgagtgct caagccagat
     5161 ttgccagcgc ttccagtgca atggaaaccg actttacagt agctgccgac ctcactgtac
     5221 atgccgttgt gtacatcaaa accaaagcca tggtatcgca acaacagcaa aacttcttta
     5281 acgatccgtt atttgatttc tttttcggtc cgcaacaagg gcaaagacaa caaaataaca
     5341 ctccgaaaca ggaagtaccc aaaggagcag gctcgggtgt aatcatcagc caggacggct
     5401 atatcgttac aaacaaccac gttatcgatg atgccagcga catagaagtc accctcaacg
     5461 acaagcggac tttcaaagcc aagcttatcg gagccgaccc ttcgaccgat ctggcactgc
     5521 ttaaaattga tgcgaacgat ctccaagcaa tcaccatcgg taactccgat aatgtgaaag
     5581 taggtgaatg ggtattggct gtgggcaatc cgttcaacct ttcgtcaacc gttactgccg
     5641 gaattgtcag cgccaaagca cgcaatatcg gaatcattgg tcagaataat ttcggcgaac
     5701 cacaatccaa caatatgtcc atcgaatcgt tcatccagac cgatgcagcc atcaacccgg
     5761 gtaacagcgg aggcgcgctt gtcaacaccc gcggtgaact cattggcata aacacagcca
     5821 tcgcatccgg aaacggcgtg tataacggat acggatttgc cataccttcg agcattgtca
     5881 gcaaggtggt taccgacctg cgtcagcacg gagccgttca gcgcgcgtta ctcggagtaa
     5941 gtattcagga tttgagcact gaagttgcca aagaaaaaaa tatcaaaaca ctcgaaggag
     6001 cattggtagc tgacgtagta tctgacggtg cagcagacaa agccggaatt aaaacgaatg
     6061 acgttatcaa cgaaatcaac ggggtgaaag taaagtcggt tgccgaactg caggaacaag
     6121 ttggtcgcta tcgtcccggc gataaagtca cagtaggttt gatgcgcgaa aacaaaccaa
     6181 tgaaactgac ggcacagctt atcaatgccg acggcaacac cgatatggta agttcgtcaa
     6241 cagtagactc cgatcttggc gccacactca aaccggttga caacaagatt aaaaaagcgc
     6301 tgggtatcga ttatggctta acagtggaat ccgtcacgaa aggcgcactc tacaatcagg
     6361 gtatccgcca gggttatatt atcctgaaaa tcaacaacca gccaatggag acgatgaaag
     6421 atttcaagga agtttacaac gaggcactca aaaaggataa aaaactgtac attgccggag
     6481 tgtatcctac caccgggaaa gtggcatact acgaaattaa acttgacaaa taaactgctg
     6541 aaaatttatc ggtcaaaaga ctgactttca cacgaaatgt cagtcttttt tcatattttt
     6601 gttacctttg aaaaaaaata aatctaatga aatctattct gaaacttttt attctgataa
     6661 tcagtctgaa cgtaagcagt ttatatgcct ctaaagttga cacagtcctc acatacagcc
     6721 cttcgatgaa aaaaagcatc aaggcggttg ttatcactcc cgacacttat acaaagaccg
     6781 aaaaaatgcc ggtactttat ctcctgcacg gatatagcgg caagtatgac aactggaccc
     6841 tacacatgcc gaatattaat tatctggccg atcagtatca gtttatcgtt gtctgtcccg
     6901 atggtaatta cggcagctgg tatttcgaca gtccgatcga cagcacattc cgttacgaaa
     6961 cctacgtagc aggcgaatta atacaatggg tggataaaaa ttacaaaacg ataaaaaacc
     7021 gcagcggaag agcaatttcc ggtctgagca tgggcggtca tggcgctctt tatctggctt
     7081 ttcgccatca ggatgtgttc ggagcctgtg gcagcatgag cggtggtgtc gatatacgcc
     7141 cgtttccaaa caactgggac ctgtccaaac gacttggcga acagcgcagt tgtccccaaa
     7201 actgggaaaa caatacggta attaatcagc ttcacctgct aacgccaaaa agtctaaaca
     7261 tcattttcga ttgcgggacc gaagatttct tttacccagt gaatgttcgt ctgcacgaag
     7321 aacttactta tcgcaacatt cctcatgatt ttatctcacg accgggacag cacaacagag
     7381 aatactggaa caatgccgta aaatatcagg cccaattctt ttctgatttc tttaaatctt
     7441 ctgaaaaaat ctaattacat tactatttca gaaagttcaa aaacaaatgc ctctgttttt
     7501 tgttttaaac cttgtaatag tagtagaagt agatattttc aaactaaact gaaggtttcc
     7561 acaacaaaaa tggtaataat gagttttatt aataataaaa taacaaatat ttggctgtaa
     7621 ctaaagttta tttacattat taattttcaa ataattaaaa attttattac ttttgcaggc
     7681 taaatttttc tccacatatg agacaattaa aaattaccaa atcaatcaca aaccgcgaga
     7741 gcgcatcact tgacaagtat ctgcaagaga tcggacgcga agaccttatt acagttgagg
     7801 aagaagtaga acttgctcag cgtattaaga acggggatcg tctggcgttg gagaaactca
     7861 cacgagcaaa tctgcgtttc gttgtttcgg tggcaaaaca gtatcaaaat cagggattaa
     7921 gccttcccga ccttatcaat gaaggcaacc tgggactgat aaaagctgct gaaaaattcg
     7981 atgaaacacg tgggttcaag tttatctcct atgctgtatg gtggattcgt cagtccatcc
     8041 ttcaggctct ggctgagcag tcacgtatcg ttcgtttacc tttaaatcag gtaggttcac
     8101 ttaacaaaat caacaaagca ttttcaaaat ttgagcagga aaatgaacgc aaaccttcag
     8161 ccgaggaact cgctgacgag cttgacattc ctgttgacaa aattgcagat acaatgaaag
     8221 tctccggacg tcatgtgtcc gttgatgctc cgtttgttga aggtgaagac aacagcttgc
     8281 ttgatgtgat gattaatgac gattcgccca acgccgaccg tgtgctgatt aacgaatccc
     8341 tgtcaaaaga aattgaacgt gcattatcaa cgctgagtga gcgtgaacac gaaattgtga
     8401 aaaaattctt tggcatcgga attccggaaa tgacactcga agaaatcggt gatgaatttg
     8461 gtctgacacg tgaacgtgtt cgtcaaatca aggaaaaagc catcagaaga ttaagacaaa
     8521 gctcaaaaag caaacttctg aagacatatc tgggctagaa aattacattg tggtaaaaac
     8581 aaatactaag caacactttc ggggtgttgc ttttttattt ttccacttca tattatttgc
     8641 tttcatactg attagcagtt actcaggaaa attatttctg atttcttctc ttaatcccaa
     8701 acaattatga aaaaaaataa tttaattttt ataattttgc acttgtttat ttgtcattaa
     8761 tccggtaagg attaaaattt gtacttctct gttttttaac agagcccttc agtttttatc
     8821 tgtttttcca aacgtaacta tttcagtata ttttagagtt tttttcacct ttactgctta
     8881 attctgagat tttttatcaa atgatgcata gcacggatat tttcttttaa aattttccga
     8941 atatttatta atacaggaat ttcatttatc actaaatata tattaatt
//
LOCUS       DAOPYB010000127         8931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9286_length_8931_cov_8.735692, whole genome shotgun sequence.
ACCESSION   DAOPYB010000127 DAOPYB010000000
VERSION     DAOPYB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8931
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9286_length_8931_cov_8.735692"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..362
                     /locus_tag="PKH58_09530"
     CDS             69..362
                     /locus_tag="PKH58_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89313.1"
                     /translation="MPSKFKGKLNLKIDEIPEGTYALEVYKVGYRSNDTYTTYLDMGK
                     PANLTKQQVEQIKSQNDGSPFMSEIIHIKKGTNFSKTLDIRENDVFLLNIIKL"
     gene            695..1525
                     /locus_tag="PKH58_09535"
     CDS             695..1525
                     /locus_tag="PKH58_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX89314.1"
                     /translation="MKLKSKLIIFLFTISSVFCYSQQINYGSNNGKFLTINGTKVYYE
                     EYGKGFPILLLHGGLGDISNFSIIIPELSKKYRVIIPDAPGLGRSEYANEILSYEMFA
                     DYNSKFIDQLNLKELFVIGWSDGANTALILTKNRPEKIKKLIISGANYKLSGFTKGAL
                     EDCKNLSDTAFVSKELKGWINHYQKLSKKDWKRYISEVGQMWFKDEYFPKSDLEKIQI
                     PTLVIYGDRDIYSLEHGIEIKNAISKSQFCVIPNCSHNVFCEKPKLFNELAITFFDEK
                     "
     gene            2106..2408
                     /locus_tag="PKH58_09540"
     CDS             2106..2408
                     /locus_tag="PKH58_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89315.1"
                     /translation="MCSNLDDIKTGGLKLFIDKQRIRIELLKDFLAGYDDGRFWGFYC
                     LACVLLPIDRLLDCQKFIHAETGTGDVKQRCTMLKDRLQQTANDLNIELRLNNRNR"
     gene            complement(2865..3419)
                     /locus_tag="PKH58_09545"
     CDS             complement(2865..3419)
                     /locus_tag="PKH58_09545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNX89316.1"
                     /translation="MKQLKIAVVVGSLRKESFNKKMAHALVENAPETLSLEFLEIGNL
                     DLYSEDLDSNPPQVWVEFRDKVKSFDGVLFVTPEYNRSFPGVLKNAIDVASRPWGQSV
                     WAGKPAAIVSVTIGGLGAFGASNHLRQVLAFLGMPTMTQPEAYIGNAMSLFDENGKLT
                     NESTLEFAKSYMKAFAQWVETFVK"
     gene            complement(3561..5252)
                     /locus_tag="PKH58_09550"
     CDS             complement(3561..5252)
                     /locus_tag="PKH58_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNX89317.1"
                     /translation="MLFACEISAQNTHARTYCNPLNVDYTYMIYNSDKDISYRSGADP
                     AVVEFRGEYYMFVTRSLGYWHSYDLQRWDFIKPEKWYFQGCNAPAAHNYKDSVLYVTG
                     DPSGSMSVLYTDNPKKGDWKAVPAILHDLQDPDLFFDDDGSAYMFWGSSNLYPIRGKR
                     LDKNKRFLPVGSVDTLFNKDMSKHGWERFGENNSDTVIAGYIEGPWLTKYNDKYYMQY
                     GAPGTEFNVYADGVYTSDHPLGPYSYAPNNPVSFKPGGFMTGAGHGSTVNGPQNKYWH
                     FASMALSYNVNWERRLCMFPLYFDKDRLMYADTYFGDYPYFGPSEPGNAGKFSGWMLL
                     SYKKPVKASSSLPDHGPEFLTDEKVKTFWVAATNDDTQWVTIDLQNQSTVYAIQINYF
                     DYKSNLYGKIDGLRHRYLIEASADGKNWETIVDKSKSYTDTPNDYVELPEAVRTRFIR
                     YKNIEVPTPHLAISDIRVFGLGQGKKPEKVAGFNVERKADRRDALITWKPTKNAQGYN
                     IRWGIAPDKLYSSWLIYDDNQLLLKALTTDQEYWFAIEAFNENGISTLSDLKHIE"
     gene            complement(5720..6892)
                     /locus_tag="PKH58_09555"
     CDS             complement(5720..6892)
                     /locus_tag="PKH58_09555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNX89318.1"
                     /translation="MQNFNFYSPTYFEFGKDKENNAGKLVKRFGGSKILIHYGGGSVV
                     RSGLLQRIKASLEKENIAYVEFGGVMPNPRSGLVYEGIDLCRKENVDFILAVGGGSAI
                     DSAKAIAIGVPYEGDFWDFYQGKRIDKALPVATVLTIAAAGSEASLSSVITHENGMVK
                     RGTGSDKIRPVFSILNPALTQTLPPYQTACGATDMFAHVLERYFTNTRNVEITDRLCE
                     GIMLTILSETPKVITNPDDYDARANIMWAGMVAHNDICGVGREQDWGTHQLEHELSAL
                     YDVAHGAGLAVMFPAWLKYNLKHDVMRYAQFATRVMGCQMNFENPEITAREGITRFET
                     FLKSIGMPVRFSELGARKEDIPTLIEMLRLGDRTVGNFVKLNRQDIEAIYRLAAED"
     gene            complement(6973..>8931)
                     /locus_tag="PKH58_09560"
     CDS             complement(6973..>8931)
                     /locus_tag="PKH58_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNX89319.1"
                     /translation="EKEIATLVVYGCTADDWKNITVNPGFTPQYVSNVHFSGTIKLGK
                     FEKVFEMDGGFRKHSGLYNCCLHNCTISDDVFIENIHNYMANYDVGKGTYIENTNLIV
                     VDGQSTFGNGVRVPVMNETGGREVPIYDYLSAPLAYILTLYRHRPAMIENLQKMIDDY
                     SESIRSDRGRIGEQVEIINCGRIKNVRIGDHSSIKGVSNLKNGSINSTAKAPVQLGSG
                     VKCTDFILCSGVTITDSTLVSTCFIGQGCIMDKHYSALDSLFFSNCQGMHGEATAIFA
                     GPYTVSHHKSSLLIAGMFSFLNAGSGSNQSNHMYKLGPIHQGIVERGSKTTSDSYLLW
                     PAKIGTFTLVMGRHTSHSDTTDLPFSYLIENATQSFLVPGVNLRSVGTIRDAQKWPKR
                     DNRKDDVKLDPINFNLLSPYTIQKMYKGIEVLENLKKISGETTEIFTYQNCKIKHSSL
                     EKGIKLYRTAIYKFLGNSLISRIQKENSASMEELQQSLASGDKNGLGDWIDLSGLIAP
                     KQVAEYLLTNIENNTLTLTDIRNRLEAMHAEYYNYEWTWAQQKLEEYWGKPIQTVGKE
                     ELTAFIELWKEAVVNLDKLLYEDARKEFNLSSKIGFGVDGDEEQKHKDFENVRGTFEK
                     NPFVQEVLHHIELKTALGNTVIEQINRL"
BASE COUNT         2513 a         1838 c         1919 g         2661 t
ORIGIN      
        1 acttttgtct caaacctttt agttaaaact gtccaataaa tagggggtca aaccattata
       61 ctcgcgactt gccttctaaa tttaaaggca aacttaatct aaaaattgat gagatacctg
      121 aaggaaccta tgctcttgaa gtatataaag ttggataccg ttctaatgat acctatacca
      181 catatttaga tatgggtaag ccagcaaact taactaaaca acaagtcgaa cagataaaaa
      241 gtcaaaatga tggctctcca ttcatgtctg aaataataca cataaaaaaa ggaacaaact
      301 tttcaaagac gcttgatatt agggaaaatg atgtttttct tttaaatatt atcaaattat
      361 aaatacaact ttctggcact aagatttgat taatatgcct ttccttcctg acaaggcaca
      421 ttggctaaga acacaagcca gcgcttcaaa cagatttttc caaatagcct ttctgcccat
      481 cccgctcaga gagagatgtg gcgattaaaa cataccgcca gtgcctaaca gccgtttaac
      541 cacataagct aagtaagtgg cacagtagat ataaacgtaa gccgaaattt gttatctttg
      601 atggcaaaaa tggagtggtg cattgccttt gatggcttat cggtaaacag tcgtaggtaa
      661 tgctaaaaaa acacgtacag tacaaaataa atttatgaaa ttaaagagca aattaattat
      721 ttttttattt acaatatctt ctgttttttg ctattcgcaa cagataaatt atggttcaaa
      781 taatggaaag tttttaacaa ttaatggaac gaaagtttat tatgaagagt atggaaaagg
      841 atttccgatt ttattacttc acggaggttt gggagatatt agcaacttta gtataatcat
      901 tcccgagctt tcaaaaaaat accgagttat tattcctgat gctcctggac ttgggcgttc
      961 ggaatatgca aatgaaatat taagttatga aatgtttgct gattataact ctaaatttat
     1021 tgaccaatta aatttgaaag aattatttgt aattggttgg agcgacggtg caaatacagc
     1081 attaatttta acaaaaaaca ggcctgaaaa aattaaaaag ttgataattt caggtgcgaa
     1141 ttataaatta agtggtttta caaaaggagc attggaagat tgcaaaaatt tatctgatac
     1201 agcatttgta agcaaagagc ttaaaggttg gattaaccat tatcaaaaat tatcaaaaaa
     1261 agactggaaa agatatattt cagaggtagg acaaatgtgg tttaaagatg agtattttcc
     1321 aaaaagcgat ttagaaaaaa ttcaaattcc aactttggta atttatggag acagagatat
     1381 ttatagctta gaacatggaa tagaaattaa gaatgctatt tctaaaagtc agttttgcgt
     1441 tattccgaat tgttcacaca atgtattttg tgaaaaacca aaactcttta acgaattggc
     1501 aattacattt tttgacgaaa aataaaaatc ggtaaaagca ctgcttacaa tatcggtttg
     1561 gcaataaggc ggctgaattt aaattaggag caattctgtc aacctcttca gctgataatc
     1621 gaattattta ttttggagat gccaacggac aagtttatgc atataaacta aagtaaaaaa
     1681 acttatgcta atacaacgat aaattttatt gccgttcttg taaatacaac aaagtactct
     1741 acgcgccaca acggtggcat aaatcaacag gaaaacgact ccgaaatcgg caactaaatt
     1801 tagcaccaaa cgttagaaat aatgttacaa agcacatttt aaagttttac ggcatgaaga
     1861 aagaaaatac aatcggctgc tgtggaattg tttgcagact ttgtccctga ttctataaca
     1921 tcgggtcttc ggcttgtcag gaatgttgta gcatgaattt cagggaaaaa catccgacat
     1981 acggtttcgt tatctgctgc gtgaataaca gagggttaga gacatgtgca gattgtgatg
     2041 atttttcatg ttcccgattt gataaggaac gtgactagtt cgattttttt gtcactcacc
     2101 aaaaagtgtg ctccaatctg gatgacatta agactggcgg actgaaatta ttcatcgaca
     2161 aacagcgaat ccggattgaa ctattaaagg attttttggc aggatacgac gatggacgat
     2221 tctggggttt ttattgtctt gcctgcgtgt tactgccgat agaccggttg ctggattgtc
     2281 agaaattcat ccatgccgaa acaggtacgg gagatgtaaa acagagatgc accatgctca
     2341 aagacagact tcagcagaca gcaaacgacc tgaatattga actaaggctc aataaccgaa
     2401 atagatgaat tcatcagaca gtaactcaat gaaaggtgta attctatgaa gattgattgt
     2461 cctacgttgc agcacaattg cttcttatgg ctccctgaat gtttagcggc ccaaaataaa
     2521 cataaactta ctttaccgtg agtacggaat aaaaactcca cccaaaaaag ttatgggagg
     2581 ctgaaaaaca ataaggtaat aaggttttaa ttcgtgttat ccggattact actaaggttt
     2641 attcaaagaa ataaggtttt tagcacggta aaagacctta ttttttatat ttcaacctta
     2701 atcgcatttg aatttgtata tgatttgaat cttattacct tattttcata gaagtcgtcc
     2761 tgaccattta taatgccgcc tctcacgttg cgtgttttaa aaaacttcct gtttcgcaca
     2821 aacgaaacag gaagctatga atttttttag gtaaaccgga tttattattt tacaaatgtc
     2881 tctacccact gagcgaaggc tttcatatag ctttttgcaa attcgagggt tgattcattc
     2941 gtcagtttgc cgttttcgtc aaacaggctc atagcgttgc cgatgtaagc ttcgggttgt
     3001 gtcatggttg gcataccgag gaatgccagc acctgacgca ggtgattgct agcaccgaat
     3061 gctcccagac ctccaatggt tacacttacg atggctgccg gttttcctgc ccacacgctt
     3121 tgtccccagg gacgcgatgc tacatcaatt gcatttttca gcacacccgg gaaagaacgg
     3181 ttgtattcgg gggtgacgaa aagtactccg tcgaagcttt ttaccttatc gcggaattcc
     3241 acccacactt gcgggggatt ggaatccagg tcttcggagt acaaatccag gtttccgatt
     3301 tcgagaaatt ccagactaag ggtttcggga gcattttcta ctaaggcgtg tgccattttt
     3361 ttgttaaacg attctttgcg caagctgcca actactacag cgattttcaa ttgtttcatt
     3421 gtttctgaat ttatttattt tttttgtttg taattttata ttctgcatta caaacaaagt
     3481 acaccgcgta aaagtttatc gatttttgtt tatgtagtat aaatagaatt tattagttct
     3541 cgaaaaatta agtttgtgtt ctattcaatg tgtttgaggt cggatagtgt agaaataccg
     3601 ttttcattga aagcctcaat ggcaaaccag tattcctgat cggtagtcag tgctttgaga
     3661 agcagttgat tgtcgtcgta gatcagccac gaagagtaga gcttatcggg agctataccc
     3721 cagcggatat tatatccctg agcattcttg gttggtttcc aggtgatgag cgcatcccgg
     3781 cggtcagctt ttctttcaac attaaatccg gcaactttct ccggtttctt tccctgtcca
     3841 agtccgaata cccggatgtc cgatatagcg agatgtggtg ttggaacttc aatgttttta
     3901 taccggataa atcttgttct tacagcttcc ggaagttcca cgtaatcatt gggagtgtcg
     3961 gtatagcttt tacttttatc aacaatggtc tcccagttct ttccatctgc cgaggcttca
     4021 atcaggtagc ggtgtctcag tccgtcaatt ttgccgtaga gattagactt gtaatcgaaa
     4081 taattgatct gtatggcata aacggtcgac tgattttgca aatcaatcgt gacccattgt
     4141 gtgtcatcgt tggtggcggc aacccagaaa gtctttacct tttcatccgt tagaaattcg
     4201 ggaccgtgat cggggagtga tgaggaagct tttacgggtt tcttatacga aagcagcatc
     4261 cagccgctga attttcctgc attgcccggc tcggaaggtc cgaaataggg ataatcgccg
     4321 aaataggtat cggcatacat cagcctgtct ttatcgaaat acaacggaaa catgcacaga
     4381 cgtcgttccc agttgacatt gtacgacaat gccatggatg caaaatgcca gtatttgttt
     4441 tgcggaccgt ttaccgtgct gccgtgtccg gctccggtca taaaaccacc cggtttgaag
     4501 ctgacgggat tatttggcgc atacgaataa ggtcccagcg ggtgatctga cgtataaaca
     4561 ccgtcggcat agacattaaa ttcggtgccg ggcgctccgt actgcatata gtatttatcg
     4621 ttatattttg tgagccacgg accttcgata taccctgcaa tcacggtgtc gctgttgttt
     4681 tcgccgaaac gttcccagcc gtgtttgctc atatccttgt tgaaaagtgt atctaccgaa
     4741 cctacgggga gaaaccgttt atttttatcc agccgtttac cgcgaatggg gtataggttg
     4801 gacgaacccc aaaacatata tgctgaaccg tcatcgtcga aaaacaaatc ggggtcctgc
     4861 aaatcgtgga gaatggcagg aactgctttc cagtcacctt ttttgggatt gtcagtgtaa
     4921 agtacactca tggaacccga aggatcgccc gtaacgtata gcacagaatc tttataattg
     4981 tgggctgcag gagcattaca accctgaaaa taccatttct cgggtttaat gaagtcccag
     5041 cgttgcagat cgtaagagtg ccagtaaccc agcgagcgag taacaaacat ataatattcc
     5101 cctctgaatt ccaccactgc aggatcggct cccgaacgat acgaaatgtc tttgtcggag
     5161 ttataaatca tgtaggtgta atccacgttg agaggattgc agtaggttcg cgcatgtgta
     5221 ttctgagcgg aaatttcaca tgcaaataac atccaaatgc caattgaaag caaatacttt
     5281 aatgaagtct tcatggtgta attttataag ttaatccgca aaatacacaa ataaaatctt
     5341 aggggtttca cccctaaaac ccgacttact tttttgtctt gccacaaaaa agtaagcaaa
     5401 aaaagtcaag actgcgcccg cttcactcga aaaattagcg ctcgactggc taaatcgtcc
     5461 aaacttgttg aagttcgtcc aaactttcca cattttggaa aatagcatca cgaacttcaa
     5521 cttcaaacaa ggacgatttt caacgcccgt ctcacttatt tttcggctca ccggacgagg
     5581 tcgctcgtta gcgagccatt ttgaaattgt cgttacatca tgcagataaa tatataatcc
     5641 aacatgaata ttcagagtgc aaccgacaaa aaaaggaaat taaacagcat ttaatttcct
     5701 tttaatgaat tgttgagaat tagtcttcgg cagcaagcct gtaaatggct tcaatatcct
     5761 gccggttaag ttttacaaag tttccaaccg tgcggtcacc gagcctgagc atctctatca
     5821 gggtgggaat gtcctctttt ctggctccga gttcgctgaa ccgtaccggc attccgattg
     5881 atttcaggaa tgtttcgaac cgtgtaatcc cttcacgggc tgttatttca ggattttcga
     5941 aattcatctg gcaccccatc acacgagtgg caaactgtgc gtaacgcatc acgtcgtgtt
     6001 tcaggttgta tttcagccag gcaggaaaca ttacagccag cccggctccg tgagctacgt
     6061 cgtagagtgc cgacagttcg tgctccagtt ggtgcgttcc ccagtcctgt tcgcgtccca
     6121 caccacagat atcgttatga gccaccattc ccgcccacat gatgttggca cgggcatcgt
     6181 aatcatcggg attggttata actttgggtg tttcgctgag gatggtgagc ataatgcctt
     6241 cacagagccg gtcggtgatt tccacgttgc gggtattggt gaagtagcgt tcaagcacat
     6301 gggcaaacat atcggttgca ccacaggcag tctgataggg cgggagtgtt tgtgtcagcg
     6361 ccggattgag gatagagaat accgggcgta ttttatcgct tccggtacca cgtttcacca
     6421 taccgttttc atgagttatc acagacgaaa gtgaagcttc gcttccggca gcggcaatgg
     6481 taaggacagt cgccaccggc aatgctttat caatcctttt accttgatag aaatcccaga
     6541 aatcaccttc gtagggaacg ccgatggcaa tagctttggc tgagtcgatg gcactgcctc
     6601 cgcctaccgc cagaatgaaa tcaacgttct ctttcctgca gaggtcaatt ccttcgtaaa
     6661 ccagtccgct tcgcgggttg ggcatcacac ctccaaattc aacatatgca atattctctt
     6721 tttccagaga tgctttgatc cgttgcagta atccgctgcg aactaccgaa ccgccaccgt
     6781 aatgtatcag gatttttgag ccgccgaatc gtttaacaag tttaccggca ttgttttctt
     6841 tatctttccc gaattcaaag tatgtcggac tgtaaaaatt aaagttttgc attcgttatt
     6901 atgttattga gttgataaag ttgataaagt gaaaagatat acaattacac actacacatt
     6961 acacgtcaaa catcaaagcc ggttgatttg ttcgatgacg gtgtttccga gtgctgtttt
     7021 gagttcgatg tgatggagaa cttcctgaac aaaaggattt ttttcaaagg tgccgcgtac
     7081 attttcgaag tccttgtgtt tctgttcttc gtcgccatcc accccgaatc cgattttgga
     7141 actcaggtta aattcctttc tggcatcttc gtagagtagt ttatccaggt tgacaaccgc
     7201 ttccttccat aattcaataa atgccgtaag ctcttctttc ccgactgttt gtatcggttt
     7261 tccccagtat tcttccagct tttgctgtgc ccaagtccat tcgtagttgt agtattcagc
     7321 gtgcattgct tccaggcgat tgcggatatc ggtcagggtc agggtattgt tctcaatatt
     7381 ggtcagtagg tattcagcta cctgtttagg agcgatcagc ccggataggt caatccagtc
     7441 acccagtccg tttttgtcac cggatgcaag cgattgctgc aactcttcca ttgatgcgga
     7501 attttctttt tgtattctgc tgatgagtga attaccaaga aacttataaa tggctgtacg
     7561 gtaaagcttt attccttttt cgagcgatga atgttttatt ttgcagttct gataggtgaa
     7621 aatctctgtt gtttcgcccg atatcttttt caggttttca agcacctcta tacccttata
     7681 cattttctgt atggtgtagg gactgagcag gttgaaattg atggggtcga gtttaacatc
     7741 atcctttcgg ttgtcgcgtt tcggccattt ctgtgcatcg cggatagtac cgacactccg
     7801 aagatttaca cccggcacca gaaaactctg tgtggcattc tcaataaggt aagagaaagg
     7861 cagatctgtg gtgtcggagt gcgaggtatg acgtcccatg acgagggtaa atgtgcctat
     7921 cttggcaggc cagagcaggt aggagtcgct ggtggttttt gatccgcgtt ccacaatgcc
     7981 ctgatggata ggtcccagtt tatacatgtg gttgctttga ttggaaccgc tgccggcatt
     8041 gaggaaggag aacattccgg caataagaag gcttgactta tggtgtgata cggtataggg
     8101 tccggcaaat atcgcggtag cctcaccgtg catgccctgg cagttggaga agaacagcga
     8161 atcaagggcc gaataatgct tatccatgat gcaaccctgc ccgataaagc aggtggatac
     8221 cagcgtggag tcggtgatcg tgactccgga gcaaagaatg aaatcggtac acttgacacc
     8281 cgaaccaagc tgaactggcg ctttggctgt gctgttgatg gaaccattct tcagattaga
     8341 aactcccttg atgctgctat gatcgcctat ccgtacattc ttgatgcgcc cgcaattgat
     8401 gatttccacc tgttcgccta tcctgcctct gtctgaccgg atactttcgg agtaatcgtc
     8461 aatcatcttt tgaaggtttt ctatcatggc gggacgatga cggtacaggg tgagaatgta
     8521 agccagcgga gccgacaggt aatcgtagat gggtacttca cgtccgcccg tttcgttcat
     8581 caccggaaca cgcactccgt tgccaaaagt tgactgtccg tcaacaacta tgaggttggt
     8641 gttttcgatg tatgtgccct tgcccacgtc gtagtttgcc atgtagttgt ggatgttttc
     8701 gatgaataca tcgtcactga tggtacagtt gtgcaggcaa cagttataca gtccgctgtg
     8761 cttgcgaaaa ccgccgtcca tttcaaatac cttttcgaat ttacccaatt ttatggttcc
     8821 ggagaaatga acatttgaaa catattgcgg tgtaaatccc gggttaacgg ttatattttt
     8881 ccagtcgtcg gctgtacatc cgtacaccac cagtgtggct atttcctttt c
//
LOCUS       DAOPYB010000128         8863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9388_length_8863_cov_12.322207, whole genome shotgun sequence.
ACCESSION   DAOPYB010000128 DAOPYB010000000
VERSION     DAOPYB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9388_length_8863_cov_12.322207"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PKH58_09565"
     CDS             <1..606
                     /locus_tag="PKH58_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013671876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HNX89320.1"
                     /translation="SIFISDTKINDDYKPILRGKDVYRYSYKESNKFIYYGNHLACPR
                     RKEIFEQPKILIREAGSVITATYDDSNFYIMSSLYNAISKDKGFNLKYLLVLINSQLF
                     QFLMNQFTFEKTKGAFTKAKIYHYYNLPVKIIQNQNPFIELVDQILLLKKENPQSDTT
                     ALEQQIDNLVYRLYDLTYDEVKVVDPAFALSEEEYNNIEIE"
     gene            820..1799
                     /locus_tag="PKH58_09570"
                     /pseudo
     CDS             820..1799
                     /locus_tag="PKH58_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451238.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            complement(2053..4818)
                     /locus_tag="PKH58_09575"
     CDS             complement(2053..4818)
                     /locus_tag="PKH58_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNX89321.1"
                     /translation="MKNALTFLLLILTFIFNSCVENKKDFIIGVSQCSDDAWRRSMNE
                     EMQREASFSGNIDLRIKTAYDNNQRQIRDIEELIAENVDLLIVSPNEAAPLTPVIEKA
                     MDEAIPVILVDRKIASNKYTAFVGADNFQIGKEVGIYASQLLNGKGNVVEIRGLKGST
                     PDLERHNGFVSSLSHFPEIKIVYESDGAWLRSEANKRMSEALSKVQSIDLVFAHNDEM
                     AIGVYEALNSSNDIKKPIILGIDALPGADGGIERVLNGSLDATFIYPTGGEKAIQTAL
                     QILNKKPFSRENVLYTAVVDKTNARVLKLQTDQIIQHQNRISKLNEILNINLSQYTTQ
                     RMLLLSSLVVLGVILLLLILLFRSYRHKNRVNSQLEKTNDEINRQKEVLSEQRDQLVT
                     LSKNMEDATQAKLVFFTNISHEFRTPLTLISGPLDNIAANEQLSTEGKKMVRMMKRNV
                     HVLLRLIDQIIEFRRYENGKMQMFFRYDDLKKFIGEICGSFRELSRKKRINLKYVVDN
                     ADFMVWFDTDKVEKICYNLISNALKFTHENGNVTVHLSKELINDEEYAKITVTDTGIG
                     ISNDHLSEIFSRFYRVDNSYSGSGIGLALTKALVEQHNGNIGVESSVGQGSTFYFSIP
                     FKQKDISVTEQYPVLQVNNVNLDDVFLADMEDETEVAEDQNRESNKQQILIVEDNYDV
                     RGYVKSILQAEFDIIEASNGKEGLLKAMRKIPDLIISDVMMEGMNGFELCKAIKENIN
                     TSHIPVMLLTAYALDEQRVTGFQSGADAYIPKPFNEELLKIRVRKLIENREKLKEYFK
                     KNLTFGEKKESVAEIDKTFMDKFRQIVEDNLLNSELSVDDIGKSIGMSRVQLYRKMKS
                     LTNYAPNELVRNIRLKKAEQLIINSDKNISEIAYETGFSSPSYFSKCFKEYFNESPKE
                     FISRMKE"
     gene            complement(4927..5799)
                     /locus_tag="PKH58_09580"
     CDS             complement(4927..5799)
                     /locus_tag="PKH58_09580"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HNX89322.1"
                     /translation="MKNIIVGLGEILWDLLPGGKVLGGAPANFAYQTSQFGFEGYAVS
                     AIGKDALGDEIIESLSSKSLNHKIERVDFPTGTVQVTLDGKGIPQYEICENVAWDNIP
                     FTADIENLARKASTVCFGSLAQRSEVSHKTILRFLELLPEDALKVFDINLRQHFYSKE
                     IIDNSLKHSNILKINDDEVVIVAELYGWNGLKEAEICKKLVEMYHLKLVILTKGTNGS
                     YVITSGEEFFRPTPIVTVADTVGAGDAFTAGFVASLLRGKTIPEAHKTAVDVSAFVCT
                     QHGAMPVLPDTLIQ"
     gene            complement(5970..7142)
                     /locus_tag="PKH58_09585"
     CDS             complement(5970..7142)
                     /locus_tag="PKH58_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX89323.1"
                     /translation="MKNKKLGLALFSTMLAFFAMGFVDLVGIATNYVKADFQLTDTVA
                     NLFPSMVFFWFLIFSIPTGMLMNKIGQRKTVLLSLVVTIAALIIPMINYSFVSMLISF
                     SLLGIGNTLMQVSINPLLTNIVSGDRLASSFTLGQFVKAIASFSAPLIAAWAATSLGN
                     WKYLFPIFMIVAIAAILLLGLTKIEEKDAKNKKSSTFLQCFSLLGDKVILLSFLGIVC
                     HVGIDVGVNVTAPKIIMERLNMSLAEAGFATSLYFLFRTIGSFSGAIILTKFSTNKFF
                     AISVVLMVLSIIGLFTFRGLIPIYISIALVGLGNSNVFPIILSKAMLYMPEKKNEVSG
                     LMIMGLFGGTIFPLLMGVMSDALASQIGALIVLSIGIIYLVFLSFKLQKDQVNLAE"
     gene            complement(7215..>8863)
                     /locus_tag="PKH58_09590"
     CDS             complement(7215..>8863)
                     /locus_tag="PKH58_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HNX89324.1"
                     /translation="LLPIQEKSPEVKVTVNYPDSISQSPYFIRLAQDSIDYWVKLDVK
                     NFKGKTVSLEFEGLEKNSFALKNIKQSDSFDFDYKEKYRPAYHFTPVYGWMNDPNGMV
                     YLDGEYHLFYQYNPFGAMWGNMHWGHAVSTDLTSWTYLPVAIGPDSLGAIFSGSAVID
                     KYNTAGFGENAMVAIYTSAGKVQTQSIAYSTDKGRTFTKYDKNPVIANPDIPDFRDPK
                     VFWHKESNQWVMALATKQTITFYGSPNLKEWSRLSEFGEGIGDHGGVWECPDLFPLSY
                     NGKTKWVLLVSINPGGPNGGSATQYFVGDFDGKTFKPDALPYPLWIDYGRDNYAGVTW
                     SNIPEADGRRIFIGWMSNWDYANQVPSKYFRSANTVPRELKLASNGNHLILTSYPVKE
                     INALKNKEKNLIDDVTVKAEYRIEKLLSVNEGTYEIEFTLQPENTDIFGFSLENTKNE
                     KIEVKFDKAAKQIILDRRTSGLSAFKDNFASEMKAPLTAGKEYRIRLLVDKASVELFV
                     NEGETVMTSIFFPTENMNTLRLFTAKGEMKVSGIRISGIK"
BASE COUNT         2672 a         1789 c         1611 g         2791 t
ORIGIN      
        1 agtatattta tatctgatac taaaattaat gacgattaca aaccaatttt acgtggaaaa
       61 gatgtctatc gctattctta taaagaatca aataaattta tttattatgg taatcattta
      121 gcttgtccac gacgcaaaga gatttttgag caacctaaaa tattaattcg agaagctggc
      181 tcagtaatta cagctactta tgatgattcg aatttttata ttatgtcatc tttgtataat
      241 gcaatttcta aggataaagg ttttaatttg aaatatttat tggttttgat aaattctcaa
      301 ctttttcaat ttctcatgaa tcaatttact tttgaaaaaa ctaaaggagc cttcactaaa
      361 gcaaaaatat atcattatta taatttacca gtaaaaatta ttcaaaatca aaatcctttt
      421 attgaactag ttgaccaaat cttattatta aaaaaagaaa atccacaatc cgacaccacc
      481 gcattagagc aacaaatcga caacctggtt taccgtcttt acgatttaac ttacgacgaa
      541 gtaaaggtgg tagatcctgc cttcgcatta tcagaagagg aatataacaa cattgagatt
      601 gaataatgct tcggcaggca ggccggagtt tgcagtcacg gaagaagaat atgcagccat
      661 agagatacat tagagatagc ctctctttgt gagagactat ctcttcaaat ttgaagaatt
      721 tagaagcagc catagagata cattagagat agcctctctt tgagagagac tatctcttca
      781 gaattgaagg atttagaaaa ggaaaaaatt gattaagata tggaaaacaa tgaaatggca
      841 tttgtgatat tcaaaacgga agacgaaaaa attgcggtgg atgttcgttt tgaagaagaa
      901 acggtttggc tttcgcagga gcaactggcg gtattgttcg aaagggacag gagtgtcata
      961 gtaaagcata ttgccaatat attttcggaa ggggaattgg aagaaaaaag caatgtgcaa
     1021 tttttgcaca ttgcaaattc ggataagcct gtaaagtttt acaacctgaa tgttattatc
     1081 tctgttggtt accgggtaaa atcgcaacgg ggtacacagt ttcgtatatg ggccacccag
     1141 cgcctcaatg agtatatccg taaaggtttt accatggacg atgagcggct gaaaaatctg
     1201 ggcggcggaa gttattggaa agaactgctg caacgtgtcc gcgatattcg tgcttccgag
     1261 aaagtatttt accgccaggt gctcgacatc tatgccacca gtatcgatta cgaccccaaa
     1321 gcagatgttt ctgttgaatt tttcaaaaaa gtgcaaaata aaattcatta tgccgttcac
     1381 ggacaaacag ccgccgaagt tattttcacc cgtgccgatg ccgaaaaaga atttatggga
     1441 ttgatgaatt tccccggcag ccgtccgtat ttgaaagatg tggtggtagc caaaattaat
     1501 tagatgaacg cgagctgcgt tcactcggac agcttgtttc cggatatttg gattttgccg
     1561 agcggcaagc cgaacgcgag caggcaatga ccatgcatga ctgggctaaa catttggata
     1621 atatactaac catgagcggt gaaaaactat tgcagggagc aggttctgtc agtcacgaaa
     1681 aagcgattga taaagcaacc accgaataca aaaaatatca acagaaaaca ttgagcgaag
     1741 ttgaaaagag ttttttagaa acgttgaaag ccatagaaaa caaaaataaa aaggaatagt
     1801 tgaggtattt ttatttcagt tgtttccaac atggaaacag ctcaaaaaca aagcataaac
     1861 acacaatatg aaaagaaata aaactacacc ggcaaacccc ttactggaac aaagaacgaa
     1921 aaatcaggta cctggatgaa gcaggattta tcctcagcga actcgcggac ggtaatgaat
     1981 atttggccag gaggctggag gggaagattg gggagtatgg gaggtatata aatggttata
     2041 aacgatagca actcattctt tcatcctgct aataaactcc ttaggacttt cattgaaata
     2101 ttctttgaaa catttagaaa agtacgatgg agatgaaaag cctgtttcat aagcgatctc
     2161 tgaaatgttt ttatcggaat taattattaa ttgttctgct ttttttaatc ttatatttct
     2221 caccaattcg ttcggagcat agtttgtcag cgatttcatt ttacgataaa gttgtacgcg
     2281 ggacattcct attgactttc cgatatcatc tacactcaac tcgctattta aaagattgtc
     2341 ctccactatt tgccgaaatt tgtccataaa tgttttatca atttctgcta cactctcttt
     2401 tttttcacca aaagtgaggt ttttcttaaa atactccttc agtttctctc tgttttcaat
     2461 aagttttctt accctgattt taagcaattc ttcgttgaat ggtttgggaa tataggcgtc
     2521 tgccccactc tgaaaaccgg taacacgctg ttcgtcgagg gcgtaggccg tcagcaacat
     2581 caccggaata tggcttgtat ttatattttc ctttatcgct ttacaaagtt caaatccgtt
     2641 cattccctcc atcatcacat cgctgataat taaatcggga atttttctca ttgctttcaa
     2701 caatccctct ttcccgttgg atgcttcgat aatatcgaat tcggcttgca ggatggattt
     2761 aacatatccc ctgacatcgt aattatcttc aacaattaga atttgttgct tattactctc
     2821 ccggttttga tcttccgcta cctccgtttc atcttccata tcggctaaaa acacatcgtc
     2881 cagattgaca ttattcacct gcagcaccgg atattgttct gttaccgaaa tatctttttg
     2941 tttgaatgga atagaaaaat aaaatgtact tccctgccct accgaacttt caacacctat
     3001 attcccatta tgttgttcta ccaatgcttt ggtaagagct aagccaattc cggaacccga
     3061 atagctgtta tccacccgat agaaacgact gaaaatttca gaaagatgat cattcgatat
     3121 accaattccg gtatcggtaa cagttatttt ggcatattct tcatcattaa taagttcctt
     3181 cgacaaatga acagtgacat tcccattctc atgtgtaaat tttaatgcat tggatatcag
     3241 attgtagcag attttttcca ctttatcggt atcaaaccat accataaaat cagcattatc
     3301 cactacatat ttcagattaa tacgtttttt tctgctcagt tctctgaacg aaccacaaat
     3361 ttcaccgatg aattttttca ggtcgtcata tctgaaaaac atctgcattt tcccgttttc
     3421 atatcttctg aattcgataa tctggtcaat tagcctcaaa agtacgtgca cgttcctttt
     3481 catcattctc accatctttt tgccttctgt tgacaattgt tcattcgcag ctatattgtc
     3541 gagcggaccg ctgattagcg tgagtggagt acgaaattca tgagagatat tggtaaagaa
     3601 tacgagtttt gcctgggtgg catcttccat atttttggaa agtgtaacca attgatcgcg
     3661 ttgttcggaa agcacttctt tctgccgatt gatttcatcg ttggtctttt ccaattgact
     3721 gtttacccgg tttttatgtc gataagatct gaaaagcaat atcaggagca ataatatcac
     3781 acccaaaacg accaatgaac ttaatagcag cattcgttga gtagtgtact gcgataagtt
     3841 aatattcagt atttcattga gtttgctgat tcggttctga tgctggatta tctgatcggt
     3901 ttgtaatttc aaaacgcgtg cattggtttt atcaactaca gctgtataaa gcacattttc
     3961 ccgggaaaat ggttttttat tcaaaatttg aagtgccgtc tggatggctt tttcaccacc
     4021 ggtaggatat ataaatgttg catcaagtga gccattgagt accctttcta ttccaccatc
     4081 ggctcccggt aaagcatcta ttcccaaaat gatgggtttc tttatgtcat ttgatgagtt
     4141 taacgcctcg taaaccccga tggccatttc atcattatga gcaaatacaa ggtcaatgga
     4201 ctgtactttg cttagcgctt ccgacatccg cttatttgcc tcactccgca gccaggcacc
     4261 atcgctttca tatacgattt tgatttcggg aaaatgactt aatgagctta caaacccgtt
     4321 atgacgttcc aaatcgggtg ttgatccttt aagtccacga atttcgacaa cattaccttt
     4381 tccgttaagc aattgggaag cgtaaatgcc aacttctttt cctatctgaa aattatcggc
     4441 tccaacgaat gccgtgtatt tattagatgc aatttttctg tccaccaata ttaccggaat
     4501 tgcttcgtcc atcgcttttt ctatcaccgg tgtcaacggt gcagcttcat tgggcgaaac
     4561 gatgagtaaa tctacatttt cagcaattaa ttcttcaata tcccgtattt gtctttgatt
     4621 attatcgtaa gctgttttaa ttctcaaatc aatatttcca ctaaaggatg cttcccgttg
     4681 catttcttca ttcatcgaac gacgccacgc atcatcactg cactgtgata cgccaatgat
     4741 aaaatccttc ttattttcaa cgcaggaatt gaaaataaaa gtaagtatca gcaataaaaa
     4801 agtcagtgca tttttcatca cgaatgattt tttgaagtaa tggatacaaa tttactgaat
     4861 agatatcaat aaaaacagaa acaaaccaac tattctgatt tgtttctgtt tatgggcgga
     4921 aattgattac tgaattaatg tatccggtaa aaccggcatg gctccatgtt gcgtacaaac
     4981 aaaagcggag acgtcaacag ctgttttatg cgcctccggg atggttttcc ccctgagcag
     5041 cgaggctaca aatccggctg taaaagcatc tccggcaccc acggtatcgg ctacagtcac
     5101 tataggtgta ggtctgaaga attcttcacc ggaggtaatc acataacttc cgttggttcc
     5161 ttttgtcaga ataactaatt ttaaatggta catttcaacc aattttttgc aaatttctgc
     5221 ttccttcaga ccattccacc cgtaaagttc agctacaatt acaacttcat catcgttgat
     5281 cttcaggatg tttgagtgtt tcaatgaatt atcaataatc tctttggaat agaaatgctg
     5341 acgtaaatta atgtcaaata ctttcaatgc atcttcgggt aaaagttcta gaaaacgtaa
     5401 aatcgtttta tgtgaaactt cacttcgctg tgccagtgag ccaaaacaaa cagtgcttgc
     5461 ctttcgtgcc agattttcga tatcggcagt gaaagggatg ttgtcccagg ctacattttc
     5521 acaaatttca tattgaggaa ttcctttccc gtccagcgtt acctgtacag ttcccgtagg
     5581 aaaatccaca cgttctattt tatgattaag cgatttgctg ctcaaacttt caatgatttc
     5641 atcgcctaaa gcatccttac ctatggcact taccgcatat ccttcaaatc caaactgtga
     5701 ggtttgatag gcaaaattgg ctggcgctcc gccaagtact tttccacccg gaagtaaatc
     5761 ccagagtatt tcaccaagac cgacaataat gtttttcata tttttataat ttttatttct
     5821 gccccctaac cccctaaagg gggaatattg agcgtctatc atttgttatg cgttatttaa
     5881 tgatgccatt gtgtgtctaa aatttttagc tggacatggc tgtttcatat gttattctgt
     5941 tttttttata aaattcaata ctcttccatc tattcagcaa ggttcacctg atctttttgt
     6001 aatttgaagg acaagaacac taagtaaata ataccaatgc tcagtacaat caaagcacca
     6061 atctgcgaag caagcgcatc agacatcact cccatcagca gcgggaatat tgttccaccg
     6121 aaaagtccca taatcatcag accggatact tcatttttct tttcaggcat gtaaagcatc
     6181 gcttttgaca aaataatcgg gaaaacattt gaatttccca atcctaccag cgctattgat
     6241 atataaatcg gaattaatcc cctgaatgtg aataagccaa taattgaaag taccatcaac
     6301 actacgctga tagcaaaaaa tttattggta gagaattttg ttaaaataat tgcacccgaa
     6361 aaacttccga tggttcgaaa caaaaagtac aagctggttg caaatccggc ttcagcaaga
     6421 gacatgttca gtctttccat tattattttc ggagcagtta catttacgcc cacatcaata
     6481 cctacatggc aaactattcc cagaaatgac agcaatatta ctttatcacc caacagagaa
     6541 aaacattgca gaaatgtgga ggattttttg tttttggcat ctttttcctc aattttggtc
     6601 agtccaagca gcaaaatggc agcaatagct acaatcataa aaatggggaa aaggtatttc
     6661 cagttgccca acgaagtggc agcccaggca gcaatcagcg gagctgaaaa tgaggcaatt
     6721 gcctttacaa actgacctaa tgtaaatgaa ctggccaatc tgtcgccact tactatatta
     6781 gtcaacagcg gattgatgga aacctgcata agcgtatttc caatgcccag caatgagaat
     6841 gaaatgagca tcgaaacaaa tgaatagttg atcatgggta taattaaagc tgcaatcgta
     6901 acgacgagac ttaataatac cgtttttcgt tgtcctattt tattcatcag cattccggtc
     6961 ggaattgaga atatcaggaa ccagaaaaat accatggatg ggaataagtt cgcaactgta
     7021 tcagtcaact gaaagtcagc ttttacataa ttggtagcaa tacccaccaa atctacaaat
     7081 cccatcgcaa aaaaagctaa catggtggag aatagagcta atccaagctt tttgtttttc
     7141 atatcttttg tttttaagtt ttattttttt tttttggaaa taaggatata attggtttaa
     7201 ggatcaaaca gaaattattt aattcccgaa attcgaatac cggatacttt catttcacct
     7261 tttgctgtaa ataatctcag cgtattcata ttttcggttg ggaagaaaat ggaagtcatc
     7321 actgtttctc cttcatttac aaaaagttct acggatgctt tatccaccag caagcgaatg
     7381 cgatattctt ttccggctgt taaaggtgct ttcatttctg aagcaaaatt atctttgaat
     7441 gctgaaagac cgctggttcg tctatccagt ataatctgtt ttgcagcttt atcaaacttc
     7501 acttctattt tttcattctt cgtattttcc agtgaaaatc caaatatgtc agtattttca
     7561 ggttgaagtg taaattcaat ttcgtaggtt ccttcattta cactcaacag tttttcaatt
     7621 cgatattcag ctttaaccgt aacatcatca atcaggtttt tctctttgtt tttcaatgca
     7681 ttgatttctt ttaccggata acttgtcaat attagatgat ttccgtttga agcaagtttc
     7741 agttcacggg gaacggtatt agcacttctg aaatatttgg atggaacctg attggcataa
     7801 tcccagttgc tcatccaccc gatgaaaatt ctgcgtccat ctgcttccgg tatattgctc
     7861 caggttacac ccgcatagtt atcacgtccg taatcaatcc aaagaggata aggaagtgca
     7921 tcaggtttga aggttttgcc atcaaagtcc cctacgaaat actgtgttgc cgaaccacca
     7981 tttggaccac cggggttaat gctcactaag agaacccatt tcgtttttcc attgtaagat
     8041 aacggaaaca aatcaggaca ttcccatact cctccatgat ctccaatgcc ttcaccaaat
     8101 tcacttaatc ggctccattc tttcagattg ggtgagccgt aaaacgtgat agtctgcttg
     8161 gttgccagtg ccattaccca ttggttgctc tctttgtgcc agaaaacttt gggatctctg
     8221 aaatcaggta tatccggatt ggcaatcacg ggatttttgt catacttggt aaatgtgcgc
     8281 cctttgtcgg tactgtaagc aattgactgc gtctgaactt ttccggcact ggtgtaaata
     8341 gctaccattg cattctcgcc aaatccggcc gtattgtatt tgtctataac tgcacttcct
     8401 gagaatattg ctcccagact atcgggcccg atagccacag gcagatacgt ccaggatgtg
     8461 agatccgtgc tcactgcatg tccccagtgc atattgcccc acatggcacc aaatggattg
     8521 tattgataaa aaaggtgata ctcaccgtca agataaacca ttccgttggg gtcgttcatc
     8581 cagccatata ccggtgtgaa gtgatatgcc gggcgatatt tttctttata atcaaaatcg
     8641 aaactatctg attgtttgat attctttaag gcaaaagagt ttttctccag cccttcaaat
     8701 tccagggaaa cagttttacc cttaaagttt ttcacatcca gttttaccca ataatcaatg
     8761 ctatcctgag ctaaccggat aaaataagga gactgtgata tactgtcggg ataatttacg
     8821 gtaactttta cttcagggga cttttcctga atgggtaaaa gca
//
LOCUS       DAOPYB010000129         8803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9470_length_8803_cov_9.092936, whole genome shotgun sequence.
ACCESSION   DAOPYB010000129 DAOPYB010000000
VERSION     DAOPYB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8803
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9470_length_8803_cov_9.092936"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKH58_09595"
     CDS             complement(<1..370)
                     /locus_tag="PKH58_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNX89325.1"
                     /translation="MLTLTDFQQIYARHPQIEALTLWAATHEQNVKISGLCGSSLSVV
                     AASLFTKAPHIQVFVMKDADEAAYLYNDLRQILSDERVLFFPSSYKKAIRLSQLDASN
                     EILRTEVLNRLVSSSTPCVIV"
     gene            606..1292
                     /gene="aqpZ"
                     /locus_tag="PKH58_09600"
     CDS             606..1292
                     /gene="aqpZ"
                     /locus_tag="PKH58_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNX89326.1"
                     /translation="MKRNLAEFFGTFWLVLGGCGSAVLAAGVPDVGIGYAGVALAFGL
                     TVLTGAYGLGHISGAHFNPAVTFGLWAGGRFNAKDILPYVISQILGAILAATVLYVVV
                     VSSGMEIGSFAANGYGELSPNGYSLAAGIITEFVMTFMFLLVILGATDERAPKGFAGL
                     AIGLALTLIHLISIPVTNTSVNPARSISQALFVGGTYITQLWMFILVPVVAAMCAGVV
                     YRFFKAEVSK"
     gene            complement(1374..3437)
                     /locus_tag="PKH58_09605"
     CDS             complement(1374..3437)
                     /locus_tag="PKH58_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89327.1"
                     /translation="MNKFKSVLITCMVTVIAVAGCKSGNNGKETMKQKNNITESTVEK
                     VSSALIDSLGASAKFRVERGVKQVAALWTEADGSETEFAQFCRENFISDTLKLDVLYK
                     RLEENFETINGYLHQIDVKLKEPIQLTGYDVTPVDMLFGGYDITAHLDEDLYANKVAF
                     ITALNFPFYTLEEKTKLGDTWSRKQWAYARMGDRFISRVPSDVLQHLSKTLTDADSYI
                     SNYNIFMGQLVDNKGVKLFPVDMQLITHWGLRDELKSDYADKLNGNAKQEMIYEVMKR
                     IIDQSIPQEVINKADYTWNPVTNKIYQNRKEITPNAENNKRYEVLLNNFKANRQIDAF
                     SPNYPDAISRNFDAAMEIPLKDVESLFRTLLASPQVKEVAKLIQSKLGRELRPYDIWY
                     NGFKAKGAISEDDLTKITAAKYPNPKALEKDLSTILIKLGFKTEKAQQIASLVQVDPS
                     LGAGHAWGAMMKGDKAHLRTRIGSKGMDYKGYNIAVHEFGHNTEQTITLNDVDYWMLN
                     GVPNNAFTEAVAFMFQKRDLELLGMKNTNPDANSYLALDNFWSCYEIMGVALVDIAVW
                     QWMYANPDATPAQLKDAVIEEAKKVWNQYYAGILGGKDEPILGIYSHMIDYPLYLSYY
                     PLGHLIDFQVEQQMKGKNFAEELQRMYTQGRIVPQIWMKRSVGSAISVEPTLKAVDEA
                     LKVVE"
     gene            3742..4404
                     /locus_tag="PKH58_09610"
     CDS             3742..4404
                     /locus_tag="PKH58_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNX89328.1"
                     /translation="MLIKVCGMIYPDNIRSVAGLFPDFMGFELFPASPRYAGNNIDIF
                     TLKHIPEKIKKIGVFVNEDLETIIGNVCKYDLDGVQIHGADKVKVCNELKKDNLIVIK
                     AFSVMNIDNFKMTNAYSEVCDYFIFDIRKDVYGRISEKKFNWNILSEYRGKIPFLLKG
                     HMSADDVHLIKHFKHPKLAGVDVNSKFEEKLGVKDEEKLKSFIMEIRRKEDYSLIPTK
                     VV"
     gene            complement(4492..5439)
                     /locus_tag="PKH58_09615"
     CDS             complement(4492..5439)
                     /locus_tag="PKH58_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNX89329.1"
                     /translation="MKRTWILITVLCLIAGAFVSCGKKERNKKDIVIAVIPKVDNEIF
                     DQVKVSAAKAAAELGITMTWEAPTSNSGQKQKELIENLIHYKVDGILISCNDEEFLRE
                     PINEAAKAGIKVATYDSDCPVSKRIFYIGTDNEKAGKICGETLLKLYEKNKRKPEQIL
                     VLSGGTTALNMKQRLKGFRDVVGADKINGVLYSLEMADFGKELLSIKLNENKKINGVQ
                     LLWSVPVLSGVDSVPALARYMKKGGVSMFFDVSKPLLRYIKANPNCATVKQDFQAMGY
                     NGVKNLYKAIMEEKFEPEILYDVKVIDQSNAEAELKLME"
     gene            complement(5657..5878)
                     /locus_tag="PKH58_09620"
     CDS             complement(5657..5878)
                     /locus_tag="PKH58_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89330.1"
                     /translation="MNIFKLLSRLGQEVFSGGKAGEHYKTWFLNSRDNRERVHDHVYA
                     HEVKRSLIRNGSRGEGVGMGHRNKKSTAK"
     gene            6074..6781
                     /locus_tag="PKH58_09625"
     CDS             6074..6781
                     /locus_tag="PKH58_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89331.1"
                     /translation="MKKSDCEIFRVFLLSAIFSIAMGVLEAIVVIYIRMVYYPAGFSF
                     PLKFLTPEMMQVEWIREISTVIMLAVVGILSGKDNLSRFLYFLYSFAVWDITYYATLK
                     IFLDWPANLLVWDILFLIPLPWLGPVLAPVICSALMLFMTVSFMYMKKVVRDFRVYPR
                     EWVFILTGATLVILSYTQDFMTLIFQNEFNLYENSDFWKTASLYVPEKFNWLTFSTGI
                     VLILMSIIFSLYQYRKK"
     gene            complement(7065..>7382)
                     /locus_tag="PKH58_09630"
     CDS             complement(7065..>7382)
                     /locus_tag="PKH58_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX89332.1"
                     /translation="SDMLRVYELIRSRGYTIKEISEKIGVTEEYLSRSISGDPSIEIM
                     EKIARILEVKVENLLNTNHEEDELYGVVIYKKKTYKITSRQSLEKLYEDVNKGFYQVK
                     SYF"
     assembly_gap    7383..7482
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7847..8443
                     /locus_tag="PKH58_09635"
     CDS             7847..8443
                     /locus_tag="PKH58_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89333.1"
                     /translation="MKIFLKLSGLILLMFVGCTFDSEIDELNAPALNKSKDQISLQTS
                     EPKTVTVPIKADFYSIPDTILPGVGCTPTQANIIMKGGGWIGGNASHTGLVNLNESHW
                     VMINCSFDPITYQLTTLAEGKITAANKDYYLYQVTMVTQLPGGDFTGEVTINDGTGKF
                     RGATGTLMIKGLVDHITGIITFTGEGTITLVVGENHKK"
BASE COUNT         2501 a         1684 c         1587 g         2931 t
ORIGIN      
        1 tcacaataac acatggagtt gaactactga caagcctgtt gagaacttcc gttctcagaa
       61 tttcattaga agcatccagt tgcgacagac gaattgcctt tttgtaggat gaggggaaaa
      121 acagaactct ctcgtcagac agtatctgtc tcaggtcgtt gtacagataa gcggcttcat
      181 cggcatcttt cataacaaaa acctgtatat gaggcgcttt tgtgaaaagt gatgcggcta
      241 caaccgacaa tgatgatcca cacaaaccgg atattttcac attctgttcg tgcgtggcag
      301 cccagagtgt cagcgcttca atttgcgggt gacgggcata aatctgttga aaatcagtaa
      361 gagtcaacat ttaatcagct gcaaagatac aataaaatgc ataaaacata aaaaaattta
      421 ttcttcaaaa catttcatta ttaagtgtta ttacagtaaa ttagaagttt atatgaaaaa
      481 cacttgtttt cgaaaacata tttttatctt tgtttttttt attattcata aaccagacac
      541 taaaagatta ggaagtaacc gaaaatcctt acgagtaggt agtaattcaa aaaagttaat
      601 tgattatgaa aagaaattta gcagaatttt ttggtacatt ttggttggta ttaggtggct
      661 gcggtagcgc tgtgctggca gccggagttc ctgatgttgg aatcggttat gccggtgtgg
      721 cgcttgcttt cgggcttacc gttctgacag gtgcatacgg acttggccac atatcaggcg
      781 cgcatttcaa tccggcagtc acttttggac tgtgggcagg cggacgattt aatgcgaaag
      841 acattttacc ctatgttatc tctcagattc tgggtgctat cctggctgca acggttttat
      901 atgttgtggt agtttcgagc ggtatggaaa ttggaagctt tgcagctaat ggatacggag
      961 aactttcacc caacggctat tcacttgcag ctggtattat caccgagttc gtcatgacct
     1021 tcatgttcct gctggtaatt ctgggggcaa ccgacgagcg cgctccaaaa ggttttgccg
     1081 gtctggcaat cggcttggcg cttactttga tacatctcat cagcattccg gtgaccaaca
     1141 cttctgtcaa tccggcacgc agtatcagtc aggcattatt tgtgggaggt acctatatca
     1201 ctcagctttg gatgtttatt ctcgtaccgg tagttgccgc catgtgtgca ggtgttgttt
     1261 accgattttt caaagctgaa gtttcaaaat aaaccattca atcttataat aaaaaaacga
     1321 ggttcacatt ttattttatg aacctcgttt ttatgaaaat atgcttttat taattactcc
     1381 accactttca gcgcttcatc cacagctttc agtgtaggtt ctaccgaaat agctgacccg
     1441 acacttcttt tcatccagat ttgaggaaca atccttccct gtgtgtacat tcgctgcaat
     1501 tcttcagcaa agttcttgcc tttcatctgt tgttctacct gaaagtcgat aagatgtcct
     1561 agcggataat aggataaata caacggataa tctatcatgt gggagtaaat acccagtatt
     1621 ggttcatctt ttcctcccag aattccggca tagtactgat tccagacttt cttcgcttct
     1681 tctattacag catccttcaa ctgcgcggga gttgcatcgg gatttgcgta catccattgc
     1741 cataccgcaa tatccaccag cgcgacaccc attatttcat agcacgacca gaaattatcc
     1801 agtgccagat aactattagc gtcgggattg gtatttttca ttccgagtaa ttccagatcg
     1861 cgtttctgaa acataaatgc caccgcttcg gtaaaagcat tattgggcac tccattaagc
     1921 atccagtaat ccacatcatt gagtgtaatg gtttgttccg tattgtgtcc gaattcgtgc
     1981 acagcaatgt tgtatccttt gtaatccatt cctttggagc caattcgtgt acgcaaatga
     2041 gccttatctc ctttcatcat cgctccccag gcatgtccgg caccgagcga aggatcgacc
     2101 tgaacgagcg atgctatctg ctgtgctttt tcagttttga atccgagttt gatgaggatg
     2161 gtcgataaat ccttttcgag tgctttgggg tttggatact ttgcagcagt aattttagtc
     2221 agatcatctt cggatattgc tccctttgcc ttaaatccgt tgtaccagat atcgtacgga
     2281 cggagttcgc gacccaattt agactgaata agctttgcaa cttcctttac ctgtggtgat
     2341 gccagtaagg tgcggaataa actttccaca tctttcagcg gaatttccat cgcggcatca
     2401 aaattacgtg aaatagcgtc gggataattc ggggagaaag catcaatctg tcggttggct
     2461 ttgaaattat tgagcagcac ctcgtagcgt ttgttgttct cggcattcgg ggtaatttct
     2521 tttcggttct gatagatttt atttgtaaca ggattccagg tatagtcagc tttattgatt
     2581 acttcctgcg gaatagattg atcgataatt cgtttcatta cttcataaat catttcctgc
     2641 ttggcatttc cgttcaattt atcggcataa tccgatttaa gttcatcgcg caatccccag
     2701 tgtgttatca gctgcatatc gacaggaaac agcttcacac ctttgttatc aacaagttgt
     2761 cccataaaga tattgtaatt cgagatatac gaatcggcat cggtcagtgt ctttgacaaa
     2821 tgctggagca catcagaggg cacacgtgaa ataaaacgat cacccatacg ggcataagcc
     2881 cattgtttgc ggctccaggt atctcccagt tttgtttttt cttcgagcgt atagaatgga
     2941 aaattaagtg ccgtgatgaa tgccacttta ttggcataaa gatcttcatc gagatgcgcg
     3001 gtaatgtcat atccgccaaa aagcatatca acaggggtga catcataacc ggttagttga
     3061 atcggttctt tgagtttaac atcaatctga tgcagataac cgtttattgt ctcaaaattt
     3121 tcttccaacc gcttgtaaag cacatccagc ttcagggtat cactgataaa gttctcgcgg
     3181 cagaattgtg caaattctgt ttcactgcca tcggcttctg tccagagtgc cgccacttgc
     3241 tttactcccc gctctaccct gaatttagcc gatgctccaa gggagtctat taatgccgag
     3301 ctaacctttt ccaccgtact ttcggtaata ttattctttt gcttcatagt ctcttttccg
     3361 ttatttccgg atttgcatcc ggcaactgca atgacagtta ccatgcatgt gattagtact
     3421 gatttaaatt tattcattga ttatatttta agtatttaaa aaatagaatt aattcaacga
     3481 caaagatata cagaatttct tatcatcagg cattcaattg gtggataatc tgaatgttga
     3541 ccgcagaatt taactgctaa tataatccat cggacaataa ttatattcgg gataatgttt
     3601 ttttgcgatt tctgtcgaaa caaaataatg aattaaaatg tttttctatt gagaaagata
     3661 ttaatttgat ttacaactgc ttttgacaaa tatatattga gacaaatgtt gagataaacc
     3721 accttaaaaa atctaactgc catgttaatc aaagtttgcg gaatgattta tccggataac
     3781 attcgatctg ttgccggttt gtttcccgat tttatgggat ttgaactttt tccggcttct
     3841 ccaagatatg caggcaacaa tattgatatc tttacattga aacatattcc cgaaaaaata
     3901 aaaaaaattg gcgtgtttgt caatgaagat cttgaaacga ttataggtaa cgtatgtaaa
     3961 tatgatctgg acggagtaca aattcatggc gccgacaagg taaaagtatg caatgaatta
     4021 aaaaaagata atttaattgt tataaaagct ttttctgtga tgaatatcga taatttcaag
     4081 atgacaaatg catactcgga ggtatgcgat tattttattt tcgacatccg gaaagatgtt
     4141 tacggacgaa tttctgaaaa aaaattcaac tggaatatac tttcggaata ccggggcaag
     4201 ataccatttc tgctcaaagg tcacatgtca gccgatgacg tgcatctgat caagcacttc
     4261 aaacacccga aacttgccgg tgtcgatgtt aacagtaagt ttgaagaaaa attaggcgtt
     4321 aaggatgaag aaaaactaaa atcgtttatc atggaaatca gaagaaagga agattacagc
     4381 ctcataccta caaaggtagt ttaagattcc ttattatata aaatataaag gcgtttacgg
     4441 ttatgtaaac gcctttatat ttttaattac atacgattta agttgattgg attattccat
     4501 cagctttaat tcagcttcgg cgttggactg gtcaattact ttaacgtcat aaagtatttc
     4561 gggttcaaat ttttcttcca taatagcttt ataaagattt ttcactccgt tgtaacccat
     4621 cgcctgaaaa tcctgtttca cggtagcaca gttcgggttc gctttaatat atctaagcag
     4681 aggctttgac acgtcaaaaa acattgaaac gcctcctttt ttcatatacc tcgccaaagc
     4741 cggtacgcta tccactccgc tcagtacggg tacgctccag agcagttgaa cgccatttat
     4801 tttcttattt tcattgagtt tgatactcag taactctttg ccaaaatcag ccatttcaag
     4861 cgaatataag acgccattta ttttatcggc acccactaca tcccgaaatc ctttcaatcg
     4921 ttgcttcatg ttgagggcgg tagtgccgcc gcttaacacc aaaatctgct ccggttttct
     4981 tttgtttttt tcgtaaagtt tcagcagagt ttcaccacaa attttacctg ctttttcatt
     5041 gtcggtgcca atgtaaaaga tccgtttgct taccgggcag tcggagtcgt atgttgctac
     5101 ttttattccg gcttttgcgg cttcgttgat gggttcacgc aagaattcct catcgttgca
     5161 actgataaga attccatcga ctttgtagtg tatcaaattc tctatcagct ctttctgttt
     5221 ttgtccgctg tttgatgtcg gcgcttccca ggtcattgtt atgcccagtt cagcagcagc
     5281 tttggcagca ctgactttta cctgatcaaa aatttcatta tccactttcg ggataactgc
     5341 tattacaata tctttcttgt tgcgttcctt tttaccgcag gacacgaaag cacctgctat
     5401 cagacataac acagtaatta aaatccaagt tcttttcata ataattatcg tttaatgttt
     5461 gaaaatattt taagttaata acccgattgt atgcatagtc caaaacggat tttttcaatc
     5521 tgaaaaatga tttaacctgc aattcttcaa ttaaatgaca gggtaaatgt attaatcctt
     5581 tattctgatt ttcaatatca gtaaacgatt ttaatttgcg tttttatacc gttttgcaat
     5641 tctattgtat ttcggcttat tttgctgttg attttttgtt tctgtgcccc attcctactc
     5701 cttcgccccg gcttccattt cggattaaac ttcgcttcac ttcatgagca taaacatgat
     5761 cgtgaactct ctctctgtta tccctgctgt tcaaaaacca tgtcttgtaa tgttcacctg
     5821 ccttaccgcc ggaaaaaacc tcttgtccta accggcttaa taatttgaaa atattcataa
     5881 cccccgatat taagttagac tttatgagaa cagatactta ttcaatagat aaacaaattc
     5941 cgcttaattt agttgaatga ttttctatta aaaacctgtt atcggttata ttttttttag
     6001 actcagaaaa actgccaaca ttcaggtttt ccatttgtta tttatatgta cactaaatca
     6061 tcttacctta tttatgaaaa agtcagactg cgaaatattc cgcgtatttc tcctttctgc
     6121 tattttcagt atagctatgg gtgtattgga ggctattgta gttatatata ttcgtatggt
     6181 ttattaccct gccggtttta gttttccgtt gaagtttctt acgccggaaa tgatgcaagt
     6241 cgagtggatt cgtgaaattt caactgtcat tatgttagct gtggttggta ttttgtcagg
     6301 taaagacaat ttaagtcgtt ttttgtattt tctttactct tttgccgtct gggatattac
     6361 atattatgca acattaaaaa ttttcctgga ctggccagcc aaccttctgg tttgggatat
     6421 actattttta atacctctgc cctggttggg tccggtttta gcaccggtta tctgttctgc
     6481 tttgatgtta tttatgactg tttcttttat gtatatgaaa aaagttgtgc gcgatttcag
     6541 agtttacccg agggaatggg ttttcatact taccggcgcc actcttgtta ttctgtccta
     6601 tactcaggac tttatgacgt tgatttttca aaatgaattt aatctgtacg agaattcaga
     6661 tttttggaaa acagcaagtc tgtatgtgcc cgaaaaattc aactggctta catttagcac
     6721 aggaatagtg cttattctca tgagcataat cttctcgctg tatcagtaca gaaaaaaata
     6781 aattgaactt tttcttttac acaaccagcg ttcaaattcg cctgagttaa tctgattagt
     6841 ataaaagtat aaaagtataa aagaataaag atttctttca tggtttattg ttctttaatg
     6901 tcatctttat ccttcataaa tctcacaagg agaaacaatg tgagtaaaat cacacaaaag
     6961 ccaataataa taattactgt aatgctcata cccgtttcag ttttttttat ttaatccaga
     7021 aaaattgtta ttgtatgctg gtattatata attaccggat ttgttcagaa atatgacttc
     7081 acctgataaa atcctttgtt gacatcttca tacaatttct ccaaactctg cctgctggtt
     7141 attttatagg tttttttctt atagataaca acaccgtata actcatcttc ttcatggttt
     7201 gtatttaata aattttcaac ctttacctcc agaattcggg caatcttttc cataatttca
     7261 attgacggat caccggatat actccttgac agatattcct ctgttacacc aattttttca
     7321 gaaatttctt ttattgtata acccctcgac cttattaatt catatactct taacatatcg
     7381 ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgccatat ttttaactcc
     7501 ggtttatttt taatctcacc ggatgaaatg atgtgtaatt aaacgtaatt aaagcaaaaa
     7561 catcaactat cggcaaaatg aaactgtttt ataaacgaat tctttgattt taattttcag
     7621 aagttgtggt taaaatcctg caaaaaaagc ctgattttct atttttattg attgctaaag
     7681 taaatgcgga tatagcttgt gtttatatcc gatttttcct ttctgttaag gtcaacagaa
     7741 agttatactc tgcagagccg gaaaatcatt taaaatcacc atcctcaaat tccggtatat
     7801 acttatatta atcagatatt tgaatgttta tatcacaaat cagactatga aaatattctt
     7861 aaaactatcc ggtttaattt tgcttatgtt cgttggatgt acatttgatt ctgagataga
     7921 tgagttgaat gcaccagcat tgaataaatc gaaagatcag atctcattgc agacttcaga
     7981 acctaaaact gtaactgttc ctataaaagc tgacttttat tctatacccg atactattct
     8041 tcccggagtg ggatgtaccc caactcaagc taatatcata atgaaagggg gcggatggat
     8101 tggtggaaat gccagtcata caggtttggt aaatttgaat gaaagtcact gggtaatgat
     8161 caactgttcc tttgatccta ttacatatca gttgactact ttggctgaag ggaagattac
     8221 agctgccaac aaagattatt atctctacca ggtaacgatg gttactcagt tacccggtgg
     8281 tgatttcacc ggagaagtaa caatcaacga tggtacagga aaattcaggg gagcaaccgg
     8341 tactttaatg attaagggtc tcgtcgacca tataacagga ataatcacgt ttaccggtga
     8401 aggtaccatt actctggttg tgggcgaaaa tcataaaaaa taaaagataa atttcgcatg
     8461 aagtaattta tctaaatatc agcgatttat aaatattttt ttttcagaca gcgttcgtta
     8521 atttagaacg ctgttttatt tttcagaact tgtttggtga tttcttggga tagtataatt
     8581 ttcaaattgt cttctaaatc cggcattaca cacatttatt catataaatt tcatcaacaa
     8641 accggatata atggttgttg taataatgta agatacaact gaattaacca cctgcaaaag
     8701 acctatatta tggaaacgaa tgcaaatcac ccaaaaatta accggaaaga gcaggaagcc
     8761 ggtgaagaac tggaaaagtt gaaatacatt caggatcatt atc
//
LOCUS       DAOPYB010000130         8730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9569_length_8730_cov_10.963343, whole genome shotgun sequence.
ACCESSION   DAOPYB010000130 DAOPYB010000000
VERSION     DAOPYB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9569_length_8730_cov_10.963343"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PKH58_09640"
     CDS             complement(<1..718)
                     /locus_tag="PKH58_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89334.1"
                     /translation="MKKSAIFLMLIILGYLASGQIVNTAFITNDAGVYIVCSSDFKNE
                     LGGNVQNNGTIKLAGNFTNNNNFNSGLLSYVIMNGAAQTIGGTNSTTFNNLTIEGSGD
                     KTLLVNSSVANSLVMNANKILISNYNLSLSPSATIANSNNTRYVVTNGTGSLIKQSLP
                     LGADFTFPVGNSITSYKPAIIKIAGTSDNFAVRVLAGLNPTTGIDPYCVQNTWIISEG
                     TPGGTTAELNLGWNTPDQGGS"
     gene            complement(847..3315)
                     /locus_tag="PKH58_09645"
     CDS             complement(847..3315)
                     /locus_tag="PKH58_09645"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNX89335.1"
                     /translation="MKTKSTKILLKQVFEQKLLKPLLIAFSVFLTTYTYAQQGVSVNV
                     SGSAADPSAMLDISSTSKGLLIPRIALTSVNDVTTIANPAISLLVYNTNASMVGGAVG
                     FWYFNGINWVQAIGPQGIQGSVGATGATGAQGIQGLSGVNGNTGATGANGTAGVAGAS
                     GKTGATGATGAAGAQGIQGVNGNTGATGANGAAGANGATGPQGIQGIQGVNGNTGATG
                     AQGIQGIQGVNGNTGVTGATGIGTVGPAGATGATGPVGCATANTVIKSNGTTATCSQI
                     FDNGVSVGIGTTTVPQKLTVNGGIHINDGSYLDFVNWEWAMGRNICSAIGSGNRLQTL
                     GYADGVYQLIGVNGVSPTYTYQNIIEASFSTGNVGIGTANAAHRLHINNDAGESAVKI
                     GANVWSTGNNNVQYFGDGGFVFVGETYSDNVLDVYGSAGIRIFDGNNYGGAGQVLTSN
                     GTYAYWSAASTSSGWALLGNAGTSASTNFVGTTDAQELTFRTNNAERMRITLRGQLVP
                     KNTGYSIFMGESAGQADDLASNYNVFLGYQSGMANTSGIDNIAIGNRALVRNTTAEGN
                     IAIGYWSCQNQSSGSANMNTAVGYYSLYQNTGGYNTGVGYAAGAGVSGPNYTYCSFYG
                     YDADFGVSGTFTNAMALGNGATVTATNYVRIGNTSVTQIGGQVGWTNLSDGRVKDNVK
                     EDVTGLDFIMKLRPVTFNINKDKQDKILNKVDKSDYPGKYDIEKIKFSGFIAQEVEQA
                     AIETGYDFSGVHKPKHENDLYGLTYSDFVVPLVKATQEQQKIIENLKAQNASLQEKID
                     LQQKEIESQNEKIEKIRLKLGIEY"
     gene            complement(3656..3898)
                     /locus_tag="PKH58_09650"
     CDS             complement(3656..3898)
                     /locus_tag="PKH58_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX89336.1"
                     /translation="MLNFFVRLHSSNPCVRHARHRYVSSDCGFQNFDCLELFFDNRSS
                     NASAESFNAKIKAFRATFRGVRNVSFFLFRLSNIYA"
     gene            complement(4043..4306)
                     /locus_tag="PKH58_09655"
     CDS             complement(4043..4306)
                     /locus_tag="PKH58_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89337.1"
                     /translation="MVELQGGQSDVSFHWTIVANRADDYDENGAIISKYQDLRFERAP
                     DPEKLIQSDKVITPAINAAAVGTKTKISKNSSQPVSVKKETIN"
     gene            complement(4364..6544)
                     /locus_tag="PKH58_09660"
     CDS             complement(4364..6544)
                     /locus_tag="PKH58_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89338.1"
                     /translation="MITKSKDIKIRKHKLLKPLFVSLCLLLTFDSYAQQGVGINAAGA
                     PAHSSALLDLSSTSKGLLIPRVSLTSINDVTTISGPAISLLVYNTNAAMVGGAVGFWY
                     FNGINWVQALGPQGIQGITGATGAAGVAGAAGASGKTGATGATGAAGTNGTNGAVGAT
                     GATGAAGTNGTNGAVGATGATGAAGTNGINGAVGATGATGAAGTNGTNGAIGATGKTG
                     ATGATGAAGTNGTNGAVGATGATGPVGCNVNNMVIKSNGTAAVCSIIYDDGTNVGIGT
                     TTLLSKLHVIASSGTGVLGVSPTASSFGLHGFNSNATGTGVFGVGNNITGSYLSSGSG
                     GAFTGLYTGVYGRATDAGGDGVYGVGLGASGSGVYGLGSDVTTGTGVIGAGNNVTPTI
                     MTNGSGGAFSAINTGAYATASAIDGEGLYGRATGTNGTGVIGAGNNVTPSVMTNGSGG
                     AFTGLSYGAYGLGTNATNGTGVVGAGNNITTLYTMTDGSGGAFTGINKGVYGHATDVT
                     AGTGVIGTGNAVSSASVLTTGSGSAFTGVNTGTYSVATATTGDGLYAVNNAAASTGNG
                     YGVFGLTSQSSGVGVGGQNNNTAGTGVLGAGNNVSGTLLTGGSGGSFTGTTIGAAGFA
                     SNTTGTLTGGYFANSQAAGNWARVAYYNGSTAYKVYGNGLVSTIVKDTEGKMVTLHCP
                     ETPEVYFQDYGSGKLTNGSEHIQIDPIFAKNIVVDQNHPLRVFI"
     gene            complement(6774..7277)
                     /locus_tag="PKH58_09665"
     CDS             complement(6774..7277)
                     /locus_tag="PKH58_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89339.1"
                     /translation="MKKTILILFLVLFSGALFSQNSLNDDTRARIGTYIKLNPAIFDL
                     QISSKEARAVADVINKAFTSYFVVPDISLSNILDGAEVTELKRLISNLNNLLTNPPAW
                     EQLVDMQTQLKNTPPTPPVFAGEVAAKKFYNPGSDEKTRNFDGKNAIPLNSSTSHEKV
                     IEIPPKK"
     gene            complement(7297..>8606)
                     /locus_tag="PKH58_09670"
     CDS             complement(7297..>8606)
                     /locus_tag="PKH58_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HNX89340.1"
                     /translation="TGATGAQGIQGIQGVNGNTGATGAQGIQGIQGVNGNTGATGANG
                     AVGAAGASGKTGATGATGAQGIQGIQGVNGNTGATGAQGIQGIQGVNGNTGATGANGA
                     VGAAGASGKTGATGSTGAQGIQGIQGIQGVQGVQGVNGNTGATGAAGTNGTNGAVGAT
                     GATGPVGCANTDYVIKSDGSAATCTEIHENSNRNIGIGTTANDNYKMYIYRPSTSYGA
                     DKAGIYAYRTGTSGATNGGTSWTYYGVDAAIKGYSYWGNEYTAGVAGYNFLDYPGSAG
                     VVGADAYDYVRAELAANSLTYNGSTSNSFYSLKLTGDFYNQEMEYGQSTLPYFNAPYN
                     LRTVNITTHGTGTTNSIVWIHGEVDFYKTSTNTFVCFYIYRDGVRLCEISEICYYDED
                     KTVHIQWMDEPAAGPHTYELWTYYPSGGMTYYGHQLHVVELKR"
     assembly_gap    8607..8706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2330 a         2064 c         1617 g         2619 t
ORIGIN      
        1 aagagcctcc ctggtcgggt gtattccagc ccaggtttaa ttcagcagta gtcccgccgg
       61 gggtgccttc gcttattatc catgtattct gaacgcaata aggatctatg cctgtagtag
      121 gatttaagcc ggctaaaact cttaccgcaa aattatctga tgttccggca atcttaatga
      181 tggccggctt ataactggtg atgctgttcc caacaggaaa agtaaaatcg gccccaagcg
      241 gaagagattg ttttattaaa cttcctgtac cgtttgtaac aacatatcgt gtattattcg
      301 aattcgcaat cgtcgcactt ggagacaagg aaagattata atttgatatt aaaatcttat
      361 tcgcattcat cacaagggaa ttggcaacag acgaattcac caaaagtgtc ttatcacctg
      421 agccttcaat ggtcagatta ttgaaagtgg tggaatttgt accgccgata gtctgtgcag
      481 ctccattcat gattacatag ctcaggagcc ctgaattaaa gttattattg tttgtaaagt
      541 taccagctaa tttgatcgta ccattgttct gcacattccc acccaattca tttttaaagt
      601 ccgaactgca aactatgtaa actccagcat catttgtgat gaatgctgtg ttgactattt
      661 gtccggaggc cagatatcct aagataatta acattaaaaa aattgctgat tttttcataa
      721 tctatatttt ttatttatca aaagattttt ttacttatat taaagtacaa atataattta
      781 aaaaacgaat aataaaaaac ttttaacatt tttattttaa aaaatgtttt tttccacatt
      841 ttctgcttaa tattctattc ccaattttag tcttattttt tcaattttct cattttgaga
      901 ttcaatttct ttttgctgta aatcaatttt ttcttgtaat gaagcattct gcgcttttaa
      961 attttcaata attttttgtt gttcttgtgt tgcttttacc aagggaacca caaaatcaga
     1021 atatgtaagg ccgtacaggt cattttcgtg ctttggttta tgaacgccgc taaaatcgta
     1081 tcctgtctcg atggctgcct gttcaacttc ctgggcaata aaaccactaa attttatttt
     1141 ttcaatatca tatttcccgg gataatcact cttgtcaact ttgttcagaa tcttgtcttg
     1201 tttgtcttta ttaatattaa aggtaacagg gcgtaatttc atgataaaat ctaatccggt
     1261 aacatcttcc ttgacgttat cttttaccct gccgtctgac aggtttgtcc aaccaacttg
     1321 tccaccgatc tgagttacgg atgtatttcc aattcttaca taattggtgg ctgttacggt
     1381 tgctccatta cccaatgcca ttgcgttggt gaaagttcct gatacgccaa agtctgcatc
     1441 gtagccataa aacgagcaat aagtatagtt cgggccactt acccctgctc ctgcagcata
     1501 acctacgccg gtattatagc ctccggtatt ttgatacaag gaataatatc cgacggctgt
     1561 attcatgttc gctgaacctg atgattgatt ttggcatgac cagtatccga tagcgatatt
     1621 tccttcagca gtggtatttc taaccaaagc tctatttcca atagctatat tgtcaatacc
     1681 tgaagtgttt gccattccgc tttgatagcc aagaaataca ttgtaattcg aagcaaggtc
     1741 gtcggcttgt cctgcacttt cacccataaa tatggaatac cccgtatttt taggtactaa
     1801 ttgtcctctc aaagttattc tcattcgttc tgcattattc gttctgaaag ttaattcctg
     1861 tgcatctgtg gttcccacaa agtttgtgga ggcgcttgtt ccggcgttgc ctaagagtgc
     1921 ccagccgctt gaggttgatg ctgccgacca gtaagcataa gttccgttag aggtaagcac
     1981 ctggcctgca cctccataat tgtttccatc aaaaatcctg atacctgctg aaccatacac
     2041 atctaatacg ttgtcgctat atgtttcacc aacaaataca aagccgccat caccgaaata
     2101 ctgaacgtta ttattgccgg tactccatac attggcgcct attttgacgg cagactcacc
     2161 agcatcgtta ttgatatgaa gtctatgcgc ggcgtttgct gttcctatac cgacattccc
     2221 tgtagagaaa cttgcctcaa taatattctg ataggtataa gttggcgaga caccatttac
     2281 tccaatgagt tgataaacgc catcagcata acccaaggtt tgtaaccggt tacctgagcc
     2341 tatcgcggag caaatatttc tgcccattgc ccactcccag tttacaaaat ccagataact
     2401 gccatcatta atgtggatac caccattaac tgtaagtttt tgaggaaccg tagttgtccc
     2461 aataccaaca gaaaccccgt tatcaaaaat ttggctgcag gtggctgttg taccattgga
     2521 tttaataact gtgttggcgg tagcacaacc cactgggcct gtagcacctg ttgcaccggc
     2581 tgggccaaca gtgccaatac ctgtagcacc tgtaacgcca gtatttccat tgacgccctg
     2641 aataccctgt ataccttgag cacccgttgc gcccgtattg ccatttacgc cctgaatgcc
     2701 ttgaatacct tgaggccctg tagcgccatt agcacctgca gcaccattag caccagttgc
     2761 acctgtattt ccatttacac cctgaatacc ctgggcgcca gcagcacctg ttgcaccggt
     2821 ggcacccgtt tttccggagg caccagcgac accagccgta ccattagcac ctgtggcgcc
     2881 ggtatttcca ttgactcccg aaaggccctg aataccctga gcaccagtgg ccccagtagc
     2941 tccaacagat ccttggatgc cctgagggcc tattgcctgc acccaattaa taccgttaaa
     3001 ataccaaaaa ccaacagccc cgccaaccat tgaggcattt gtattataga ccagcaaaga
     3061 aatcgcggga ttggcaatcg tggtgacatc attaactgaa gttaatgcaa tacgcggaat
     3121 taaaagccct tttgacgtac tgcttatatc cagcatggcc gaaggatctg cggctgaacc
     3181 agaaacattg accgatacac cttgttgtgc atacgtatat gttgtcaaaa acacgctaaa
     3241 tgcaattaat aaaggtttta ataacttctg ctcaaaaact tgttttaata aaatttttgt
     3301 agattttgtt ttcatgatta attaatttta tttatattcc gttcaatgtt tttatttttt
     3361 cttctttttt tcaatgatac aaacataatt aaataatttt acgttcataa atttaatttt
     3421 acattttttt ccacaatggt aatatgccat tgtttgtatg ttttcttaaa ataattcttt
     3481 catttctttt atcatttatt catctcactc cctgtgtgtt gtgatccaat ttagttagaa
     3541 tcgatgttct aaaaatcttc aaattttcag tctaaaatgt tgcatttatg aattttacat
     3601 tcaacattcc taaaaaataa attgggatca atcggaaaag ttgaggagtt atttattaag
     3661 cataaatatt tgataatctg aaaaggaaaa atgagacatt ccttactcct ctaaaagttg
     3721 ctctgaaagc ttttattttg gcattgaaag attcagcaga agcattagaa ctcctgttat
     3781 caaagaaaag ctcgagacag tcgaagttct gaaaaccgca gtctgagctt acatacctgt
     3841 gccgagcatg ccgaacgcaa ggatttgaag aatgaagacg aacgaagaaa tttaaaattt
     3901 tcagaggtgc tctttattaa gagttccaat attaatcaga gtttgtccat aatgtccgac
     3961 ttcatccccg cctgatccgc cagttggcgg acagtcgggc attcgaacat tctggttcaa
     4021 actcagctgt tttggacaga cactaattaa ttgtttcctt ttttacggat accggctgcg
     4081 atgaattctt actaatttta gttttggttc caacagcggc agcatttatt gctggtgtga
     4141 ttactttatc ggattgtatt aatttttcag ggtcaggggc tctttcaaac cggaggtcct
     4201 ggtattttga aataatagct ccattttcat cataatcatc agcacggttg gcaacaatag
     4261 tccaatggaa cgacacatca gactgtccgc cctgcagttc aaccacatcg aatcctgtct
     4321 gggtttcatt ggtagtgtaa acgcccttac aatcaccttg taactaaatg aataccctta
     4381 aaggatgatt ctggtctacc acaatatttt ttgcaaatat cggatcaatt tgtatatgtt
     4441 cgctgccatt tgtcaatttt cccgaaccat aatcctgaaa ataaacttcc ggagtttcag
     4501 ggcaatgcaa agtaaccatt ttcccttcgg tatctttgac aatggttgat acgagaccat
     4561 ttccatatac cttgtatgca gtggaaccat tataataagc cacacgcgcc cagttgccag
     4621 cagcttgtga attagcaaaa tatccacctg tgagagtacc ggttgtattt gatgcaaatc
     4681 cagccgcacc gatagttgtt cctgtaaatg aaccgccgct gccgcccgtt aacaaggtgc
     4741 cgctaacatt attccctgca ccaaggaccc ctgtaccggc agtgttatta ttttggccgc
     4801 ctacaccaac gccagaactc tgcgaagtaa ggccaaagac tccatacccg ttacctgtgc
     4861 tggctgctgc gttattaacc gcataaagcc catccccggt tgtggccgtt gccacagaat
     4921 atgttcctgt gttaacgcca gtaaaagcgc tcccgcttcc ggtggtcaac actgatgcgg
     4981 aagacaccgc gtttccggtg ccaatcactc ctgtgccagc ggttacgtca gtagcgtggc
     5041 cgtacacacc tttgtttatg cctgtaaaag ccccgccgct gccatcagtc attgtgtata
     5101 atgtggtaat attatttcct gcaccaacaa cacctgtacc attggtggca tttgttccca
     5161 aaccgtaagc accataactt aaaccggtaa aggcgcctcc gctgccattg gtcataaccg
     5221 acggcgtaac attattccct gcacctatta cgccggttcc attagtaccg gtggcacggc
     5281 cgtataatcc ttcgccatct attgccgatg ccgtggcata agcaccggta ttgattgcag
     5341 aaaaggctcc gccgcttcca ttggtcataa tggtaggtgt tacattgttt ccggcgccta
     5401 ttaccccggt gcccgtagtt acatcactac ccaggccata aacgccgcta ccgcttgccc
     5461 caagtccaac gccgtaaaca ccatcaccac cagcgtcggt ggcacgacca tatactcctg
     5521 tgtacaatcc ggtaaatgca cccccactgc cggaactcag gtatgaaccg gtaatattat
     5581 tgccaacgcc aaaaacacca gttcctgtgg cattggaatt aaagccatgt aagccaaaag
     5641 agcttgctgt tggactaaca ccgagaaccc cggtgccact tgaagcaatt acatgaagtt
     5701 tcgaaagtag tgttgttgtc ccgattccca cattagttcc gtcatcatag attatgctgc
     5761 atactgcggc ggtaccattg gatttaataa ccatattatt tacattgcaa cccacaggac
     5821 ctgtagcacc cgtagcgcca acagcgccat tagtaccatt tgttcccgct gcgccagtag
     5881 cgccagtggc cccggttttt ccggtggctc cgatagcgcc atttgtacca ttagttcccg
     5941 ctgcaccggt agcgccagtg gctccaacag caccatttat gccattagtt cccgctgcac
     6001 ctgtagcccc agtggcacca acagcgccat ttgtaccatt agttcccgct gcacctgtag
     6061 ccccagtggc accaacagca ccattcgtac cattagttcc tgctgcaccg gtagcgccag
     6121 tggctccggt tttccctgag gcgcccgctg cacctgcaac tcctgcagca cccgttgcac
     6181 cggtaatgcc ctgaataccc tgagggccta atgcctgcac ccaattgata ccgttaaaat
     6241 accaaaaacc tacggcaccg ccaaccattg cagcatttgt gttatatacc agcaatgaaa
     6301 ttgcaggccc tgatatggtg gtaacatcat taattgaagt taaagataca cgtgggatca
     6361 aaagcccttt agaggtactg cttaaatcca gcaaagctga agaatgagca ggcgcgccag
     6421 cggcgttaat tcccacaccc tgttgtgcat aactatcaaa tgtcaacaac aagcataatg
     6481 aaacaaacaa tggctttaac aacttatgtt tcctgatttt aatgtctttt gactttgtta
     6541 tcatacaatt gttttttatt gttattttat acttttactt aaatttattt cacaaatata
     6601 ataatacaat ctcctataca aaattattaa aactattatt tggactataa caaattaagt
     6661 ttaataactt cattgctttt ataccttaac atcatggcta taattgtttt gtacaattaa
     6721 gggttcagct atctaatcgt ttccttcact ttttgtgtta attgaaatga cagttatttc
     6781 ttgggtggaa tttcaataac tttctcatgc gatgtactgg aatttaaagg tatcgcattt
     6841 ttcccatcaa aattacgggt tttttcatca ctaccgggat tataaaactt ctttgccgct
     6901 acctctcctg caaaaaccgg aggtgttggc ggagtatttt ttaattgcgt ttgcatatcg
     6961 accaattgct cccatgctgg tggatttgtc aaaagattat taagatttga tatcagtcgt
     7021 ttaagttcag tgacttctgc tccgtctaaa atatttgaca aagaaatgtc cggcacaaca
     7081 aaatatgaag taaatgcctt gttaatgaca tcggcaaccg cacgagcctc ctttgaactt
     7141 atctgaagat cgaaaattgc cggattcaac ttaatatatg tacctattct ggcacgtgta
     7201 tcatcgttca aagagttctg cgagaagagc gctcctgaaa ataacaccaa aaacaaaatt
     7261 aaaatcgttt ttttcataat atttaatatt taagttttat ctttttaatt caaccacatg
     7321 taactgatgt ccgtaataag tcataccgcc gcttggataa taagtccaga gttcatatgt
     7381 gtgaggaccg gcagccggtt catccatcca ctgaatatgt acggttttat cttcatcata
     7441 gtaacatatt tcgctgatct cgcataacct tacaccatcc ctgtatatat aaaaacaaac
     7501 aaaagtattg gtgctggtct tataaaaatc cacctctccg tgaatccaaa cgatactgtt
     7561 ggttgtacct gttccgtggg ttgtgatatt taccgtcctt aaattatacg gtgcgttaaa
     7621 gtatggaagt gttgattgtc cgtactccat ttcctggtta taaaaatcac cggtcagttt
     7681 aagcgaataa aaagaatttg aagtagagcc gttgtaggtc aacgaatttg ctgcaagttc
     7741 tgctcttaca taatcataag catcggctcc tacaacacca gcgctacctg gataatcgag
     7801 aaagttgtag cctgccacac ccgcagtata ttcatttccc cagtatgaat atcctttaat
     7861 ggcagcatct acgccataat atgtccagga agtacctcca ttggtagcgc ctgatgtacc
     7921 agtcctgtat gcgtaaatgc cggccttgtc agctccgtaa cttgtggatg gcctgtaaat
     7981 atacatttta taattatcat tggcggtagt tcctataccg atattcctgt tgctattctc
     8041 atgaatttcg gtacaggtgg ccgctgatcc atcagatttt atcacataat cggtatttgc
     8101 acagcccaca ggtcctgtgg ctccggtggc tccaactgca ccatttgtac catttgttcc
     8161 ggctgcgcct gtagcgcctg tatttccatt gacaccctgg acaccctgga caccctggat
     8221 accctgaatc ccctgaatac cctgtgcccc tgtggaacct gtagcgcctg tttttccgga
     8281 ggcaccagca gcgccaacag caccattagc gccagttgca cctgtatttc cattaacacc
     8341 ctggataccc tggataccct gagcacctgt tgcgcctgta tttccattga caccctgaat
     8401 accctggata ccttgagcac ctgtcgcgcc tgttgcaccg gtttttccgg aggcaccagc
     8461 ggcgccaaca gcaccattag cgccagttgc acctgtgttt ccattgacac cctgaatacc
     8521 ctggataccc tgagcgccag tagcgccagt atttccattg acaccctgaa taccctggat
     8581 accctgagcg ccagtagcgc cagtatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnttcc atttacaccc tgaatacctt
//
LOCUS       DAOPYB010000131         8658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9670_length_8658_cov_8.131233, whole genome shotgun sequence.
ACCESSION   DAOPYB010000131 DAOPYB010000000
VERSION     DAOPYB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9670_length_8658_cov_8.131233"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..446)
                     /gene="rsfS"
                     /locus_tag="PKH58_09675"
     CDS             complement(87..446)
                     /gene="rsfS"
                     /locus_tag="PKH58_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNX89341.1"
                     /translation="MIDNETIVEKIVEGIQEKKGKRIVVVDLNKLHDAPCSYFVICQG
                     DSNTQVNAIATSVKDWVTEKIKVKPYATDGFENCQWIAMDYGQIIVHVFQRPFREFYD
                     LEHLWEDADLRLIPDLD"
     gene            complement(523..1263)
                     /locus_tag="PKH58_09680"
     CDS             complement(523..1263)
                     /locus_tag="PKH58_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89342.1"
                     /translation="MKQITIFLLFLLFLTMDTFAQQTVVVSVITLKTGEKYRGEIVVR
                     NDEILMLKTEDGKRYQFMINEIEKIKQEPSAVSKKEIVSEEKTKNLFGGMLSLNAGLG
                     SAPGAIASSPVTNISVALGTHRTLGTNAFLGIGSGIETVFTKNTVLSYMPLFIGIHND
                     FGKVFACGLKTGYDFALNKLYKGGPMAEITGGISYRLTDTAGMYFGLFGQIRHINGPV
                     TEKTPWGEFTGINNDALYNLGLKASYTF"
     gene            complement(1267..2394)
                     /locus_tag="PKH58_09685"
     CDS             complement(1267..2394)
                     /locus_tag="PKH58_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX89343.1"
                     /translation="MKPFDVYPLFDIEITKGVGCYTYDKEGTEYLDMYGGHAVISIGH
                     SHPYYIQKMEEQMEKLVFYSNSVQNSLQVKLAEKLGKLSGYEDYSLFLVNSGAEANEN
                     ALKLASFHTGRKKVVAFGKSFHGRTSAAVQVTNNPKIVAPVNEGVLVDFLPLNNIDAV
                     REALKNNDVCAVIIEGIQGVGGIQIPQTDFLKSLREVCTATGTMLILDEIQSGYGRSG
                     KFFAHQHAGIRPDIITTAKGMGNGFPIGGVLISPDIKAVHGMLGTTFGGNHLACSAAI
                     AVLDVMKGEKLVENAASVGNYLIGKLKELRGIKEVRGLGLMIGIEMDGPVKELRNKLL
                     FEHKVFTGASGTNIIRLLPPLNLSKQDADLFLTRFKQALHS"
     gene            complement(2564..4621)
                     /gene="htpG"
                     /locus_tag="PKH58_09690"
     CDS             complement(2564..4621)
                     /gene="htpG"
                     /locus_tag="PKH58_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNX89344.1"
                     /translation="MAKGNIGVTSDNIFPVIKKFLYSDHEIFLREIISNAVDATQKLK
                     TLAAVGEFKGELGDLTIRVHVDPKKKKLTVSDRGIGMTAEELDKFINQIAFSGAEEFL
                     EKYKNDAQSIIGHFGLGFYSSFMVSKKVEIFTQSHKDGAVAQHWSCNGDPEYTVEDTI
                     KADRGTDIVMHIDDENKDYLEESKITELLNKYCKFLPVEIAFGKQKEWKDGKQVETDK
                     DNIINDVNPAWVRKPADLKEEDYDKFYHDLFPMSDEPLFHIHMNVDYPFNLTGILYFP
                     KIKNNIDIQRNKIQLYCNQVYVTDHVENIVPEYLTLLHGVIDSPDIPLNVSRSYLQSD
                     SNVKKISSHITKKVADRLNDIFKSDREQFEQKWDDLKLFIQYGMLSDEKFYERAEKFA
                     LLKNVDDKYFTFEEYKQLIEGNQKDKEGELIYLYTTNKDEQFTYLEAAKAKGYDVLLM
                     DGQLDVHAISQFEQKFEKTRFVRVDSDVIDKLIRKEDTGKVELTDDQRDALITIFDSQ
                     MPSMDKTNFIVTFEKLSPESNPVFITQNEFMRRMKEMSAMQGGGMMNFYGEMPDSYNL
                     VVNTAHPLIEKLMQEEQEATEAEVLPFTAQLGELKNRQTALNDSHKGKKDEEIPAAEK
                     EELDSLQQKIDELTESKKAVYKTFASGNKVVGQLIDLALLSNNMLKGEALAKFVKRSI
                     EIL"
     gene            4845..>6370
                     /gene="zwf"
                     /locus_tag="PKH58_09695"
     CDS             4845..>6370
                     /gene="zwf"
                     /locus_tag="PKH58_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNX89345.1"
                     /translation="MNDNTNMKPVNTTDNQVLVIFGANGDLSHRKLLPALYNLFYDNL
                     LPEKFAVIGSGSDPKTEQTFRQEIKEQLTVYAPHTAGNSPEKLTEFLKLLYYKPVNNK
                     VKEDFAGLNACIDEVCASLDIPRNVIYYFSIPPFMYEVVALHLVSYGMNKEEDGWKRI
                     VVEKPFGYSYDSAVELDQKLHHGFGEHQIYRIDHYLGKETVQNIMVTRFSNGFFEPIW
                     NRRYVDRVEITSAENIGVGKRGGYYDTSGAMRDMIQNHLLQVLAIVAMEPPAVFDSDS
                     IRNESVKVLQALRPISEEEVEKYVIRGQYLQTTSNDELIQGYRQEKDVAPGSKTETYV
                     AMKVFVDNWRWGDVPFYIRTGKRLPERVTEVVLYLKPAPLQLFKEANVSRFANKITLR
                     IHPDAGIAVDFGMKIPGAGYKVQNVNMDFHYSDLAEVNIPEAYERLLLDCMIGDSTLY
                     ARADAVHASWKFVDPILKAWKENPDVPLYFYESNTWGPVEANQLFENKAIKWVSPCGK
                     "
     assembly_gap    6371..6470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6471..6832
                     /locus_tag="PKH58_09700"
     CDS             <6471..6832
                     /locus_tag="PKH58_09700"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNX89346.1"
                     /translation="LASELPLNDGIPVFDLVLLGMGDDGHMASIFPDNMSLLNSVETV
                     AVVQHPQSGQGRITLTGSVIRAAKQRIFLITGKSKSDILRNIIHKEPVAELYPASHLI
                     SLPDTEVFADKKACSFL"
     gene            6886..8298
                     /gene="ahcY"
                     /locus_tag="PKH58_09705"
     CDS             6886..8298
                     /gene="ahcY"
                     /locus_tag="PKH58_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNX89347.1"
                     /translation="MNTFATLPYKVADITLADFGRKEIELAEKEMPGLMAIRQKYSTE
                     KPLKGARIMGSLHMTIQTAVLIETLVELGAEVRWCSCNIYSTQDHAAAAIAAAGVPVF
                     AWKGETLKEYWWCTLQALTFEGHQGPTVIVDDGGDATMMIHVGAAAEKNPSVLEKSVD
                     GEDERELLAILKDVLQEDNGIWNRMAPEIRGVSEETTTGVHRLYQMLEEGSLLFPAFN
                     VNDSVTKSKFDNLYGCRESLADGIKRATDIMLAGKVVVVCGYGDVGKGCAKSMRAYGA
                     RVLVTEIDPICALQAAMEGFEVCTVEDALPEGNVYVTTTGNRDIIRIEHMEQMKDGAI
                     VCNIGHFDNEIQVDKLKKYNGINHVNIKPQVDKYIFPDGHCLLLLADGRLVNLGCATG
                     HPSFVMSNSFTNQTLAQIELYTKKYEVGVYRLPKHLDEEVARLHLEQLGVKLTRLTPE
                     QAAYIGVNPDGPYKAEHYRY"
     gene            complement(8485..>8658)
                     /locus_tag="PKH58_09710"
     CDS             complement(8485..>8658)
                     /locus_tag="PKH58_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HNX89348.1"
                     /translation="ATQTLPIGIAYAVWTEIGAVGTALVGIFIFKEPATFLRLLFLTT
                     LIGSIIGLKAVSY"
BASE COUNT         2256 a         1875 c         1895 g         2532 t
ORIGIN      
        1 ataaataaca tttgaatact gtttaaattc tttttaactt acaattcaac aaccaatcgg
       61 ttggttttgt ttgcagattc gtggaattaa tccaggtcag gaataagtct caggtcagcg
      121 tcttcccaca aatgttccag gtcgtaaaat tcgcggaagg ggcgctgaaa tacatgtaca
      181 atgatttgtc cgtaatccat ggcaatccac tgacaatttt cgaaaccatc ggttgcatag
      241 ggtttcactt tgattttttc tgtaacccag tccttcacag aagtggctat ggcattaacc
      301 tgcgtgttgg aatcaccctg acagatgaca aaataactgc acggggcatc gtgtagtttg
      361 ttcaaatcca ccacaacaat tcttttacct ttcttttcct gaattccttc tactattttt
      421 tctactattg tttcgttatc aattattttt ttcatctttt ggaatagatt aattatatct
      481 tctatgtctg ttttgaggtt cattttttta gtcacttact ctttaaaatg tgtaagatgc
      541 tttcaatccc aaattgtaca gggcatcatt gtttattccc gtaaattctc cccagggtgt
      601 tttttcggtt accggtccgt tgatatgcct tatctggcca aacaaaccga aatacattcc
      661 cgcagtatca gtaagccgat aacttatacc tccggtaatt tcggccatgg gtccgccctt
      721 gtacagcttg ttcagcgcaa agtcataacc ggttttcaga ccacaggcaa atacctttcc
      781 aaagtcgtta tgtatcccga taaaaagggg catataactt aatacggtat ttttagtaaa
      841 aacggtttcg atgcctgaac caattcccag aaaagcattg gtacccaatg ttctgtgtgt
      901 gcctaatgcc accgatatgt ttgttacggg cgatgaagca atggcaccgg gagcgctgcc
      961 caatcctgca ttcaggctta acattccacc aaacaggttt ttggtttttt cctctgaaac
     1021 aatctctttt ttacttacag ctgacggttc ctgcttaatt ttttcaattt cattgatcat
     1081 aaactggtat ctttttccgt cttcggtttt cagcatcagt atttcatcgt tacgcaccac
     1141 aatttcacca cggtattttt ctcctgtttt cagggtgatt acgctcacaa ctacagtttg
     1201 ctgcgcgaat gtatccatcg tcagaaacag caagaaaagc aaaaagatgg tgatttgttt
     1261 catcaatcag gagtgtaatg cttgcttgaa acgggtgaga aacaaatcgg catcctgttt
     1321 cgacaaatta agcggtggca gcaaccggat aatattagtt ccgctggcgc cggtaaatac
     1381 tttatgttcg aacagaagtt tgttgcggag ttccttcacc ggaccatcca tctctattcc
     1441 tatcatcagt cccaatccgc gaacttcttt tataccgcgc aattctttta gttttccaat
     1501 cagatagttg cctaccgaag ctgcattttc aaccagtttt tcgcctttca ttacatccag
     1561 tacggcaatg gcagcgctgc aggccaggtg attaccaccg aatgtggtac cgagcatgcc
     1621 gtggaccgct ttaatgtcgg ggctgataag tacaccgcca atggggaaac cattgcccat
     1681 tcctttggct gtagtgatga tatcggggcg aatacccgcg tgctgatggg cgaagaattt
     1741 tccgctacgt ccgtaacccg actggatttc gtcgaggata agcatcgttc cggttgcagt
     1801 acatacttcg cggagcgatt tgagaaaatc cgtttgcgga atttggatac cgcccacacc
     1861 ctgaatgccc tcgatgataa ctgcgcatac atcgttgttt ttcaaggctt cacgtacagc
     1921 gtctatgtta ttcaaaggaa gaaaatcaac cagaacacct tcgttgaccg gagcaacaat
     1981 tttcggattg ttggtcacct gtacagctgc ggatgttctg ccgtgaaagc ttttgccaaa
     2041 agcaaccact ttttttctgc cggtgtgaaa ggaagcaagt ttaagcgcgt tttcgttcgc
     2101 ttcagctccc gaattaacaa gaaaaagtga gtaatcttca tatcccgaga gttttccaag
     2161 tttttctgcc agttttacct gaagcgaatt ctgtacggag ttggaataaa aaaccagttt
     2221 ctccatctgt tcctccatct tctggatata atatgggtgt gaatgaccaa tagatatgac
     2281 ggcatgtccg ccgtacatgt cgaggtattc tgttccttcc ttatcgtagg tataacatcc
     2341 tactccttta gttatttcaa tatcaaaaag cggataaaca tcaaatggtt tcatcaggca
     2401 aatattatta aatataagtt gcaaaagtaa cgattatttt ggtgaaaata atcaataaga
     2461 cactaagaac ctgtaaagaa gatggaaaat caacttccgg acagtgttct tagtgtctta
     2521 aagtctgtat ttgtatttta atgcaatttt acggaataaa aaactacagg atttcaatgc
     2581 tccgttttac aaatttagcc agcgcttcac ctttcagcat attgttggaa agcagtgcca
     2641 ggtcgatgag ttgtcccacc actttattac ctgatgcaaa ggttttgtaa actgcttttt
     2701 tgctttcggt cagttcatca attttctgtt gcaggctgtc cagttcttct ttttcggcag
     2761 caggtatttc ttcgtctttt ttacctttgt gactgtcatt aagtgcagtc tgacggtttt
     2821 tcagttcacc cagctgtgct gtaaacggca gtacttccgc ttcggtggct tcctgttctt
     2881 cctgcatcag cttttcgatg agcgggtgag cagtgtttac taccaggttg tagctgtcgg
     2941 gcatctcgcc gtagaaattc atcataccac cgccctgcat agccgacatt tccttcatgc
     3001 ggcgcataaa ctcgttttgg gtgatgaata ccggatttga ttcgggtgaa agtttttcga
     3061 aggttacaat aaaattggtt ttatccatcg acggcatttg tgagtcgaag atagtgatca
     3121 gtgcatcccg ctggtcgtcg gtaagttcaa cttttccggt atcttccttg cgtatcagtt
     3181 tatctattac atcgctgtcc acgcgtacga aacgtgtctt ttcgaatttc tgttcgaact
     3241 ggctgatagc gtgaacatcg agttgtccgt ccatgagcag tacatcatag ccttttgctt
     3301 tggctgcttc gagataagtg aattgttcat ccttgttggt ggtgtagagg taaataagtt
     3361 cgccttcctt atctttctga ttgccttcta tcagctgttt gtattcttcg aaagtgaagt
     3421 atttgtcatc cacattttta aggagcgcga atttttcggc acgttcgtag aatttttcat
     3481 cgctcagcat accgtactgg atgaagagtt tcaggtcatc ccatttctgt tcaaactgtt
     3541 cacggtccga tttaaagatg tcgttgagcc ggtcagccac tttcttggtg atatgcgacg
     3601 aaatcttttt tacattggaa tcgctctgca ggtaactgcg cgatacatta agcggtatat
     3661 ccggtgaatc gattaccccg tgaagcaggg tgaggtactc gggtacgata ttttctacat
     3721 gatcggtcac atatacctga ttgcagtaaa gctgaatttt attgcgctga atatcgatgt
     3781 tgtttttaat tttcgggaag taaaggatac cggtcaaatt gaacggataa tccacgttca
     3841 tgtgaatgtg gaaaagcggt tcgtcgctca tcgggaacag atcgtggtag aacttgtcat
     3901 aatcttcttc tttcagatcg gcgggtttgc gcacccaggc agggttcaca tcgttgatga
     3961 tgttgtcctt gtcggtttcc acctgtttgc cgtctttcca ttctttttgt tttccaaatg
     4021 caatctcaac cggcaggaat ttacagtact tgttgagcaa ctcagtgatt ttgctttctt
     4081 ccagataatc tttgttctca tcgtcgatat gcatcacaat gtcagtaccg cggtcggctt
     4141 tgatggtatc ttcaaccgta tattcggggt caccgttgca cgaccagtgc tgagcgacag
     4201 caccatcttt gtgcgattgg gtaaaaatct caaccttttt cgaaaccatg aatgatgagt
     4261 aaaaaccaag tccgaaatgt ccgatgatgg attgcgcatc atttttgtat ttttccagaa
     4321 attcttcggc gcccgagaaa gcaatctggt tgatgaattt atcaagttct tcggctgtca
     4381 tcccgatacc gcggtccgaa acagtcagtt tcttcttctt cggatcaacg tgcacacgaa
     4441 tggtgaggtc gcccagttcg cctttaaatt cgccgaccgc tgcgagtgtt ttcagtttct
     4501 gggtagcatc aacggcattg gagataattt cacgcagaaa aatctcatga tcactgtaca
     4561 aaaatttctt gataacgggg aagatattgt cacttgttac ccctatgttt ccttttgcca
     4621 taaattatat gttttattat tttatatgtt tatgattgtt attatccgct tcgtatgaat
     4681 cttgccgctt gttgtcagca tgtttatcgg tttgcaaata ggatgccaat cgcgtttgac
     4741 tgacagaatg gcagattttc ggaatctgtc actgtaaatt tggcaaaatt taaacaatcc
     4801 ggaagaaaat tcgttattag tttgaaatgt aaaaaccatt caacatgaac gataatacaa
     4861 acatgaaacc cgtaaacaca accgataatc aggtgttggt tatttttggt gcaaatggag
     4921 atttatcgca ccgtaaattg ctgccggctc tttataattt gttttatgac aatttgttac
     4981 cggagaaatt tgctgtgata ggttccggaa gcgacccaaa aacggagcaa acattcaggc
     5041 aggaaataaa agagcaatta actgtttatg cacctcatac ggccggaaat agccccgaaa
     5101 aattgacaga atttctgaaa ttattgtact ataaaccggt caataataaa gtaaaggaag
     5161 attttgccgg attgaatgcc tgcattgatg aagtttgtgc aagtctggat attccgcgca
     5221 atgttattta ctatttttcc attcccccgt ttatgtacga ggtcgttgcc ttgcacctgg
     5281 ttagttatgg aatgaataaa gaagaggatg gctggaagcg aattgtcgtt gaaaaacctt
     5341 ttggttacag ttacgattcg gcggttgaac ttgaccagaa actacatcat ggtttcggcg
     5401 aacatcagat ttatcgcatt gaccattatc tgggcaagga aacggttcag aacattatgg
     5461 tcacccgttt ttccaatggc tttttcgaac caatctggaa ccgccgatac gtggaccgcg
     5521 tagaaattac ttcagcagag aatattggtg tcggtaagcg gggaggatac tacgatacat
     5581 cgggtgccat gcgcgatatg attcagaatc acctgctgca ggtgctggct attgtggcca
     5641 tggaacctcc ggctgtgttt gactcggact ccatacgcaa tgaaagtgtg aaagtgcttc
     5701 aagcattgcg tcccatcagt gaagaagagg tggagaagta cgtcatcaga ggacagtatc
     5761 tgcaaaccac ctccaacgat gaactcattc agggttaccg gcaggaaaaa gacgttgccc
     5821 ccggctccaa aacagaaacc tatgtggcga tgaaagtctt tgtggacaac tggcgctggg
     5881 gcgatgtgcc tttttatatc cgtaccggaa aacgattgcc ggaacgggtt actgaagtgg
     5941 tgctctatct caagcccgcg ccactgcaac tctttaaaga agcgaatgtc agccggtttg
     6001 ccaataagat aacgctccgt attcatcccg acgccggaat tgccgttgac ttcggcatga
     6061 aaattcccgg tgccggttat aaggttcaaa atgtgaacat ggactttcat tattctgacc
     6121 tggccgaagt gaacataccc gaagcttatg aacgtctgct gctcgattgc atgatcggcg
     6181 actccaccct ttatgcccgt gccgacgcgg tacatgccag ctggaagttt gttgatccca
     6241 tcctgaaagc ctggaaggaa aatcccgatg ttccgctgta tttctatgaa agcaatacct
     6301 ggggacctgt tgaagccaat cagctttttg agaataaagc gattaaatgg gtttcgccct
     6361 gcgggaaatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgctcgcttc
     6481 tgaattgcct ctaaacgacg gaattcctgt gtttgatctg gtgctgctcg gtatgggcga
     6541 cgacggacac atggcctcca ttttccccga caatatgtca ttgctgaatt ccgttgaaac
     6601 tgttgcagta gtacaacatc cccaaagcgg acagggccgc attacactca ccggttctgt
     6661 cattcgtgct gcgaagcagc ggatatttct gattacaggc aaatccaaat cggacatttt
     6721 gagaaatatc atacacaaag aacctgttgc agagctatat cccgcttctc atctcatctc
     6781 gttgcccgat accgaagttt ttgccgacaa aaaagcatgc agttttctgt gacaaaaaaa
     6841 tatgctacct ttgcagtctc taaaactaaa cagaacaaca ttaaaatgaa tacatttgca
     6901 acattaccgt acaaagtagc cgatattacg ctggctgatt ttggacgaaa agaaatagag
     6961 ttagctgaaa aggaaatgcc gggtctgatg gctatccgcc agaaatacag caccgaaaag
     7021 ccactgaaag gtgcacgcat catgggttcg ctgcacatga ccatccagac tgccgtgctg
     7081 attgaaacac tggtggaact gggcgccgaa gttcgctggt gcagctgtaa catttattcc
     7141 acacaggatc atgccgcagc cgcgatagct gccgcaggtg ttccggtttt tgcctggaag
     7201 ggcgaaacac tcaaggagta ctggtggtgt acacttcagg cgctgacttt cgaaggtcat
     7261 cagggaccaa ccgtgattgt tgacgatggc ggcgatgcta ccatgatgat tcacgtgggt
     7321 gcggctgccg aaaaaaatcc gtccgtactc gaaaaatccg ttgacggtga agatgaacgt
     7381 gagctgctcg ctatactgaa agacgtattg caggaagaca acggtatctg gaatcgcatg
     7441 gcacccgaaa ttcgaggcgt gtccgaagaa acgaccaccg gagtacaccg tctctatcag
     7501 atgctcgaag aaggttcgct gcttttcccg gctttcaatg taaatgactc agtcaccaaa
     7561 tctaaatttg ataacctcta cggatgccgc gagtcgcttg ccgatggtat taaacgggct
     7621 accgatatca tgcttgccgg taaagtggtg gtggtttgcg gttatggcga tgtgggtaaa
     7681 ggctgtgcca aatccatgag agcttacggt gcacgtgtac tggtaaccga aatcgacccg
     7741 atctgcgctt tgcaggctgc tatggaaggt ttcgaagtgt gtacagtgga agatgcgctg
     7801 cccgaaggca atgtgtatgt aaccacaaca ggcaaccgtg atatcatccg catagaacat
     7861 atggaacaga tgaaggacgg agctatcgtt tgcaatatcg gacatttcga caacgaaatt
     7921 caggtggata aactgaaaaa atacaacgga atcaaccacg taaacatcaa accgcaggtg
     7981 gataaatata ttttccccga cggacactgc cttttactgt tggccgatgg tcgtctggtc
     8041 aatctgggat gcgctacagg tcacccttcg tttgtgatga gcaactcatt taccaatcag
     8101 acactggctc agattgaact ttacaccaaa aagtatgaag ttggcgtcta tcgtctgcca
     8161 aagcacctcg atgaggaagt tgcccgtctg catctggagc agctcggcgt aaaactcacc
     8221 cgactcacac ctgaacaggc agcatacatc ggtgtgaacc ccgacggacc gtataaagcc
     8281 gaacattaca gatattgatt ttattgtcag caacacataa gtgaaaaggc atctccgaaa
     8341 atccggaagt gcctttttct ttttatgtaa tggttgaatg aagtttagtt gtaatcgaaa
     8401 cgattgaatt tcaataccaa tctacgggtt tgactttctg tacaaacaga attccaaccg
     8461 gactttttgt tagatcataa gcttttagta cgatacggcc ttaagtccga taattgaacc
     8521 gattaatgtt gtaaggaaaa gtaatcgcag gaaagtcgcg ggttctttga atatgaaaat
     8581 tccgactaga gctgttccga ctgctcctat ttcagtccac acagcataag ctatcccgat
     8641 tgggagtgtt tgggttgc
//
LOCUS       DAOPYB010000132         8631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9723_length_8631_cov_8.752332, whole genome shotgun sequence.
ACCESSION   DAOPYB010000132 DAOPYB010000000
VERSION     DAOPYB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9723_length_8631_cov_8.752332"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="porQ"
                     /locus_tag="PKH58_09715"
     CDS             <1..1071
                     /gene="porQ"
                     /locus_tag="PKH58_09715"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNX89349.1"
                     /translation="KRVTIRNTMTRKYLPALILILSNILAVFPQAGKGVYRFLDLPVS
                     SRMAAIGGTNVSLRDNDVNFAFINPALLTSETDGVIGLNMANYLSDIKFGTAVYGHNF
                     GEKNFMSVGVQYVDLGKFDGRDELDVEQGTFTAKDMALYVSYARPLNRYFSVGATFKP
                     IVSAYERYTSVGAAFDAGISYKDSIGLFSAGLVLRNAGMQFKGYYPDEDGSQHRESLP
                     LDIQLGASKKFAHAPFRFSLTLHNLQRWDLSYTTDDTQSNFDGTTSEKTINAVDMAFR
                     HAVFGVEFVPSNNFYLALGYNHRRHQEMVMSGFKSMAGFSFGGGVKLYKFHVGFGMTQ
                     YQVGNYAYQFSISTALDEFKNL"
     gene            1126..1809
                     /gene="cmk"
                     /locus_tag="PKH58_09720"
     CDS             1126..1809
                     /gene="cmk"
                     /locus_tag="PKH58_09720"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNX89350.1"
                     /translation="MKKIIVAIDGFSSCGKSTMAKELARYAGYIYVDTGAMYRAVSLY
                     AIRNGWMTETNINEEELYKHINELKITFKTDEHGEQQTWLNGENVEKEIRTLEVANGA
                     SRVSTLGFVRRELVRQQQLMGQEKGIVMDGRDIGTVVFPGAELKIYLTASPEVRAQRR
                     YEEMMAKGEHPDYSEVLANVRERDHRDQNRSESPLKKADDAIEIDNSVLTRAQQRDEL
                     RKLFDKKTL"
     gene            1938..2810
                     /locus_tag="PKH58_09725"
     CDS             1938..2810
                     /locus_tag="PKH58_09725"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNX89351.1"
                     /translation="MANIEIDQKSGFCFGVVTAIRKAEEELAKGDVLYCLGDIVHNSQ
                     EVARLARLGLVTIGYEQFQRLHDVKVMFRAHGEPPSTYETARKNNITLIDASCPVVLK
                     LQSHIRKAYEAKDENDQLLIYGKQGHAEVIGLLGQTNDSAIIVENEDDLSKVDFSRNI
                     RLFSQTTKPLDGFNHLVKTISENISPEARFEYSDTICRQVANRIPNITNFALKNDVVI
                     FVSGKKSSNGKVLFEQSKKVNPNTFLISDPEEVDKLELDLSKNIGICGATSTPRWLME
                     AVAERIRQKEVKNE"
     gene            2860..3843
                     /gene="pfkA"
                     /locus_tag="PKH58_09730"
     CDS             2860..3843
                     /gene="pfkA"
                     /locus_tag="PKH58_09730"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNX89352.1"
                     /translation="MEYKLKCIGILTSGGDAPGMNAAIRAVTRAAIFNGISVKAIYRG
                     YKGLITGEVKEFQTQNVSNIIQQGGTILKTARCEEFRTPEGRKQAYETMKNENIDALV
                     VIGGDGTFTGARVFAQEYNIPIVGLPGTIDNDLYGTDSTIGYDTALNTIVEAVDKIRD
                     TASSHERLFFIEVMGRDAGFLALNSALASGAEAAIIPERETKVDQLAELIQNGFRKTK
                     NSSIVIVAESEITGGANGLAERMRREYPGYDVRVTILGHIQRGGSPTAYDRILASRMG
                     VAAVDALMDEQRSIMIGIVNDEIVHVPFTKAIKDDKPVNEKLLGVLQILSI"
     gene            3913..4386
                     /locus_tag="PKH58_09735"
     CDS             3913..4386
                     /locus_tag="PKH58_09735"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNX89353.1"
                     /translation="MSNKTAVLFVCLGNICRSPMAETVFNKILSDRKLSEDFEVDSAG
                     LIDYHEGEKADVRMRNHARMRGYNITHISRPVINADFERFDYIVCMDDQNIRALKSLA
                     KTVQQQRKICKISDFCSLYKVNAVSDPYYGDDAGFIHVIDLLEDACEGFLRFIRK"
     gene            4561..5214
                     /locus_tag="PKH58_09740"
     CDS             4561..5214
                     /locus_tag="PKH58_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89354.1"
                     /translation="MLENIDEELVRCRPCGYVMKKSELGTGVCPACGLPHTVFEPYRE
                     KVSPKRLFILGLDIHPIAIHLSQTFVAMIPGLMIFHLIFPNFFPEILHPVISFSVFVF
                     PLTLLLSFASGLLDGLTRFKTLETPLLKSKIIFSLLIVLLSGAIVILFKPETYNFWIV
                     LLSLAALGCAVKLGLMGKKLINVILPGTYQRRKKKVTAKAPVKTTAKLKTEDTPAAE"
     gene            5684..7177
                     /locus_tag="PKH58_09745"
     CDS             5684..7177
                     /locus_tag="PKH58_09745"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNX89355.1"
                     /translation="MEKLFIFDTTLRDGEQVPGCQLNTIEKIQVAKALEALGVDVIEA
                     GFPISSPGDFNSVVEISKAVTWPTICALTRGVQKDIEVAAEALKFAKRKRIHTGLGTS
                     EFHIKYKFNSTQDEILERAVAATKYAKKYVEDVEFYCEDAGRTDNVYLSRVVEAVIKA
                     GATVVNIPDTTGYCLPWEYGNKIKYLFENVKGIQNAIISTHCHNDLGMATANTMAGVM
                     NGARQVEVTINGIGERAGNTSLEEIAMILKCHKGLEIETQINTQKIYNTSRMVSSLMN
                     MPVQPNKAIVGRNAFAHSSGIHQDGVLKNRESYEIMDPKDVGIDENSIVLTARSGRAA
                     LKHRLSVLGVEVDNGKLDVIYEEFLKLADRKKDINDDDILVLAGMERSEQQRIKIDYL
                     QVTSGVGVKAVASLGLIIAGEKFEAAASGNGPVDAAIKALKQIIRRNMTLQEFTIQAI
                     SRGSDDVGKVHMQVEHQGSIYYGFGANTDIIAASVEAYIDAINKFVK"
     gene            7358..>8631
                     /gene="leuC"
                     /locus_tag="PKH58_09750"
     CDS             7358..>8631
                     /gene="leuC"
                     /locus_tag="PKH58_09750"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNX89356.1"
                     /translation="MRDLRGQTLFDKIWDAHVVSEVANGPTQLYIDRHFCHEVTSPQA
                     FNGLRARGLKVFRPEKTTMTADHNTPTIDQDQPVKDPVSRNQLDTFARNAAEFNMPLF
                     YPLGHEKNGVVHIIGPENGFTQPGMTIVCGDSHTSTHGAFGTIAFGIGTSEVEMVLAT
                     QCILQNRPKTMRITFNGKLGKNVLAKDLALYMISKLTTGGATGYFVEYAGEAIRNLSM
                     EERMTVCNLSIEMGARGGLIAPDETTFNYVKGREFAPKGEEWDKKLEFWKTLKTDDDA
                     VFDSELIFDASEIEPMVTYGTNPGMGMGISGKIPTLDEIDEAGRISYMKSLDYMGFKP
                     GEKMLGKPVDYVFLGSCTNGRIEDFRIFANYVKGKKKAENITAWLVPGSWQVEKQIRE
                     EGLYEILTEAGFQLRQPGCSACLAMNDDKVPAG"
BASE COUNT         2650 a         1692 c         1972 g         2317 t
ORIGIN      
        1 aaaagagtta caatccgcaa tacaatgacc cgaaagtatt tacccgcatt gattttgata
       61 ctatccaaca tattggctgt atttccacag gcagggaaag gtgtttaccg ttttctggat
      121 ttgcctgtat cttcgcgtat ggctgccatt ggcggtacca atgtatcgct gcgcgacaac
      181 gacgtgaatt ttgcattcat caatcctgca ttgctaactt ccgaaacaga tggggtgatt
      241 gggttgaaca tggccaatta tctgtcggat attaagtttg gaaccgcggt ttacgggcac
      301 aactttggcg aaaagaattt tatgtcggta ggagtgcagt atgttgattt gggtaagttc
      361 gacgggcgcg atgagctgga tgttgagcag ggaaccttta ccgccaagga tatggctttg
      421 tatgtgagtt atgcccgccc gctgaaccgt tatttttcgg tgggagccac tttcaaaccc
      481 atcgtttctg cttatgaacg atacaccagc gttggtgcag ctttcgatgc ggggatcagc
      541 tacaaggaca gtatcggctt attcagcgcc ggactggtgt tgcgtaatgc gggcatgcag
      601 ttcaagggct actatcccga tgaggatggc agtcagcacc gcgaatccct gccgctagat
      661 attcagcttg gagccagcaa gaaatttgcc cacgcgcctt tccgcttctc acttacgctt
      721 cacaacctgc agcgctggga tttgtcatat actacggatg acacacagtc gaatttcgac
      781 ggaacaacat ctgaaaaaac aatcaacgcc gtggatatgg cttttcgaca tgcagttttc
      841 ggcgtggaat ttgttccttc caacaacttt tacctggcac tcggatataa tcacaggcgt
      901 caccaggaaa tggtaatgtc gggtttcaag agtatggccg gtttctcttt tggaggtgga
      961 gtaaaactat ataaattcca tgtgggattt ggcatgacac agtatcaggt gggtaattat
     1021 gcataccagt tttccatcag taccgccctc gatgagttta aaaatttgta aagtcagcat
     1081 ttaatactga tttttttgta tcccttgtaa agaatttagt ttatcatgaa gaaaataatt
     1141 gttgcaattg atggtttttc atcttgcggt aaaagtacaa tggccaaaga actggccaga
     1201 tatgccggtt atatttatgt agatacgggc gccatgtatc gcgctgtatc tctttatgcc
     1261 atacgcaatg gctggatgac tgaaaccaac atcaacgaag aagaattgta caaacatata
     1321 aacgaactga aaatcacgtt caaaacagat gagcatggag agcagcaaac ctggctcaac
     1381 ggtgagaatg tggaaaaaga aatacgtacc ctggaagtgg caaacggtgc aagccgcgta
     1441 agtacacttg gatttgttcg ccgcgaattg gttcgtcagc aacaactgat ggggcaggag
     1501 aaaggcattg tgatggacgg acgtgatatt ggtacagtgg tttttcccgg ggctgaattg
     1561 aaaatatatc tgacagcttc gcccgaagtg cgtgcacagc gcaggtatga agagatgatg
     1621 gcaaagggtg aacatcccga ctacagtgaa gttcttgcca atgtccgcga acgggatcac
     1681 cgcgaccaaa accgttccga aagtccgttg aagaaagcgg atgatgctat tgaaattgat
     1741 aattccgtcc tgacacgtgc acagcaacgc gacgaactcc gaaagttgtt tgacaagaaa
     1801 accttataag ccggtttgtt gttattattt ttacaaaaaa aatattcacg ggacaatcta
     1861 tgctgtttta ccggaatggg cagagcctga aatgaatgtg ataatgacaa catgtataag
     1921 ataatatcag ggaaagaatg gcaaatatag aaattgatca gaaatcgggg ttttgctttg
     1981 gagtggttac tgcaatccgg aaagcggaag aagaattggc taaaggagac gttttgtact
     2041 gcctgggtga tattgtgcac aacagtcagg aagttgcccg gcttgcccga cttggactgg
     2101 taactattgg ctatgagcag tttcaacgat tgcatgatgt gaaggttatg tttcgggcgc
     2161 atggtgaacc accttcaacc tatgaaacag ccagaaaaaa caacattaca ctgattgacg
     2221 cgtcctgtcc ggttgtactg aaacttcaaa gccacatccg taaagcctac gaagcgaaag
     2281 atgaaaacga tcaactgctc atttatggaa aacagggaca tgcggaggta atcggattgc
     2341 ttggacaaac aaatgattca gctatcattg tggaaaatga ggatgatctc agcaaggttg
     2401 atttctcccg gaatatccga ctcttttctc agaccaccaa acctctcgac ggatttaatc
     2461 atcttgtaaa gacaatttca gagaatattt ctcccgaagc cagatttgaa tattccgata
     2521 ctatttgccg tcaggttgcc aatagaattc caaatattac taactttgca ctcaagaatg
     2581 atgtggtgat atttgtcagc ggcaagaaaa gttccaacgg caaagtgctt ttcgaacaaa
     2641 gcaaaaaggt taatccgaat acttttctta tttcagaccc cgaggaagtg gataaactgg
     2701 aacttgattt gtcgaaaaat atcggcattt gcggtgcaac ctccacaccc cgttggctca
     2761 tggaagcagt agcggaaaga ataagacaaa aagaagtgaa aaacgaataa cataagtaat
     2821 cctgacagtt gtaaaatgct gtcgtaagag taataagata tggaatataa attgaaatgt
     2881 atcggcatac tgacatcggg tggtgatgct cccggtatga atgctgctat acgtgccgta
     2941 acccgtgccg ccatttttaa cgggataagt gtcaaagcaa tatatcgcgg atacaaaggg
     3001 ctgattaccg gtgaagtgaa agaatttcag acacagaatg taagcaatat tattcagcag
     3061 gggggtacaa tccttaaaac tgcccgttgt gaagagttca gaacacccga aggacgcaag
     3121 caggcttatg aaaccatgaa aaatgagaat atcgatgctc tggtagttat cggtggcgac
     3181 ggaacattta ccggtgcccg tgtatttgcc caggagtata atattcccat tgttggtctg
     3241 cccggcacta tcgataatga cctctatggt acagactcaa ccattggtta tgataccgcg
     3301 ctgaatacca tcgttgaagc tgtggataaa attcgggata cggcgtcctc acacgaacgt
     3361 cttttcttta ttgaggtgat gggtcgtgat gccggattcc tggcacttaa cagtgcactt
     3421 gcctcgggag ccgaagcggc tatcattccc gaacgtgaaa cgaaagttga ccagttggca
     3481 gaactgattc agaacggttt ccgtaaaact aagaacagca gtattgttat tgttgccgaa
     3541 agtgaaatta ccggcggagc taacggactg gctgaacgta tgcgtcgcga atatccaggc
     3601 tatgatgtac gtgtaaccat tctcggacat atacagcgag gaggttcccc aactgcttac
     3661 gaccgcattc tggcaagccg catgggcgta gctgctgttg atgctttgat ggatgagcag
     3721 cgtagtatca tgattggaat agttaatgat gaaatagtac atgtgccatt caccaaagcc
     3781 attaaggatg ataaaccggt gaatgaaaaa ctacttggtg tgttgcaaat actatccatt
     3841 taaataatat caactgaaaa tagcaaacac tttcactgtt tgctattttt tttatgaaga
     3901 aataaaaaaa acatgagtaa taagactgct gtcctgtttg tctgtctggg aaatatctgc
     3961 aggtcaccta tggctgaaac agttttcaat aaaatactgt ctgatcgtaa actgtctgaa
     4021 gattttgaag tggactctgc cggactgatt gattaccatg aaggcgaaaa ggctgatgtc
     4081 cggatgcgta atcacgcccg gatgcgaggc tataatataa cacacatttc aaggcctgtt
     4141 atcaatgccg attttgagag atttgattat atagtttgta tggatgatca gaacatcagg
     4201 gcgttgaagt cgctggccaa aaccgtgcaa caacagcgga aaatctgtaa aatatccgat
     4261 ttttgcagtc tttataaagt taatgcagtt tccgatccat actatggtga cgatgccggt
     4321 tttattcacg tcattgattt gttggaagat gcctgcgaag gatttttgcg attcatcagg
     4381 aaataaaagt aaatttctaa ctaaacgcag cgacattcag gctgtaattc cttattatta
     4441 tattcccaca aaacgtttga aagaacattc tgattattta aatatttgtt tttcagtcag
     4501 aaattttgtt ttaaatatta tcttattatt tttgcataaa caatttaata attttgaatt
     4561 atgcttgaaa atatagatga agaacttgta cgttgtcgtc catgcggtta cgttatgaaa
     4621 aaaagtgagt taggtaccgg agtatgcccg gcctgcggat tacctcatac ggtttttgaa
     4681 ccctaccgtg aaaaagtgtc acccaaacga ctgtttattc ttggtctgga tattcatccc
     4741 attgccattc acctctcgca gacatttgtg gcaatgatac ccggtctgat gatttttcat
     4801 cttatttttc caaacttttt cccggaaata cttcacccgg ttatcagctt ctcagtgttc
     4861 gttttcccgc ttaccctgtt actgtctttc gcttcgggcc ttctcgatgg actgacccgt
     4921 ttcaaaaccc tggaaacacc gcttttgaaa tctaaaatta ttttcagctt acttattgtt
     4981 ttactttcgg gagcgattgt aatccttttc aagcctgaaa cctacaattt ctggatagta
     5041 ctacttagtc ttgctgccct gggatgcgct gtaaaattgg gtctgatggg aaaaaaactg
     5101 ataaatgtga tactgcccgg aacctatcaa cggagaaaga aaaaagtgac tgctaaagct
     5161 ccagtaaaga ctaccgctaa actgaaaacg gaagatactc ctgctgcaga ataaaaaaag
     5221 aaagacatct accaatatta attaaaagaa caatgacgat attgttcttt tttttgtgtc
     5281 attgtaaaac taaaataatt attaatcaga gaaattataa gataaaatat caattatatt
     5341 tcattttgtt ttgcataatt gaaaaaatga attacctttg caacgttttc ataaaataaa
     5401 caatgaacag aaactttcta catattattc tactaggtat tattattctc ttgcaatgaa
     5461 agagggagtg agcttggtat gtagttatga tataataaat aattaaaacc tttctcactg
     5521 attgagaaag gtttttttat gggttatcct aaatttatta tcacttttcg caagaatata
     5581 taaaaggacg attttgttta cttgaaataa gaaaaatatt aactaactat ttaaatgccg
     5641 ttttcctcta cggcttaccc tttttgggaa tagaggacag tatatggaaa aactatttat
     5701 tttcgataca acactgcgcg acggtgaaca ggttcccggg tgccagttga atacaataga
     5761 aaaaatccag gttgccaaag cactggaagc gttgggagtt gatgtaattg aagccggttt
     5821 ccccatttca agcccgggtg atttcaactc tgtggtcgaa atttccaaag ccgttacctg
     5881 gccaactatt tgtgctctga cacggggcgt tcaaaaggat attgaagtag ctgcggaagc
     5941 gctgaaattc gctaaaagaa agcgtattca taccggtctg ggtacttcgg aatttcatat
     6001 taaatataaa ttcaattcca ctcaggacga aattcttgaa cgggcagttg cggcaacaaa
     6061 gtatgccaaa aagtacgtgg aagatgtgga gttttattgc gaagatgccg gacgaactga
     6121 caatgtatat ctttcccgcg ttgtggaagc agttatcaaa gcaggtgcca cggtggtaaa
     6181 tatccctgac acaaccggtt attgtctgcc ctgggagtat ggcaataaaa tcaaatacct
     6241 ttttgaaaat gttaagggta ttcagaatgc aatcatctcc acgcattgcc acaacgacct
     6301 gggtatggca acagccaata ccatggccgg tgtaatgaac ggtgctcgtc aggtggaagt
     6361 aactatcaac ggaattggcg aacgggcagg gaatacctca ctagaagaga ttgccatgat
     6421 tttgaaatgc cacaagggac ttgaaattga aacacaaatc aatactcaga aaatttacaa
     6481 caccagccgc atggtttcca gcctgatgaa tatgcctgta caacccaaca aagctattgt
     6541 tggacggaat gcattcgctc actcatcagg tattcatcag gatggggtgc tcaaaaatcg
     6601 tgaaagttac gagataatgg atccgaaaga tgtcggaatt gatgaaaact ccatcgtact
     6661 gacagcacgc agcggtcgcg ctgcactcaa acacaggctt agtgtgctgg gtgtggaggt
     6721 tgataacgga aaactggacg tcatttacga ggaattcctg aaactggcag accggaagaa
     6781 agatattaac gacgatgata tcctggtact tgccggaatg gaacgctcag aacaacaaag
     6841 aataaaaatt gactatttgc aggtaacatc cggagtagga gtgaaagcag tggcaagtct
     6901 cggtttgatt attgccggcg agaaatttga agcagctgcc agtggcaacg gaccggtgga
     6961 tgcagctatc aaggcgttga aacaaatcat cagacgaaat atgaccttgc aggaatttac
     7021 aatacaggct atcagccgag gcagcgatga cgtgggaaaa gttcacatgc aggtggaaca
     7081 tcagggttcc atctattacg gatttggtgc taacaccgac atcatagccg catctgtcga
     7141 agcatatatc gatgcaatca ataaatttgt aaaataagta caccctaaat aactctcggg
     7201 ggacttgaag acattctaaa ataaaaaaag taaactctgt aataacaaga aaagaaacat
     7261 atttacttaa ttcgtgttat taatattcat tcatgtaatt cgtaaaatta aaaatatgac
     7321 aaataagata acagaccaac aagcccaaag gtcccccatg agggatttaa ggggccaaac
     7381 cctttttgat aaaatatggg atgctcacgt ggtatccgaa gtagccaacg ggccgacaca
     7441 attatatatc gaccgccatt tttgccacga agtaacaagc ccacaagctt tcaacggatt
     7501 aagggcaagg ggattgaagg ttttcagacc tgaaaaaacc accatgactg ccgaccataa
     7561 tacacccact attgatcagg atcaacctgt aaaggaccct gtgtcaagaa atcagctgga
     7621 tacatttgca cgaaatgcgg ccgaattcaa tatgccgctg ttttatccgc tcggacatga
     7681 aaaaaacggg gtcgttcaca tcattggtcc agaaaacggg tttacacagc cgggaatgac
     7741 aattgtatgc ggcgacagtc acacttctac tcacggcgct tttggtacaa tcgcttttgg
     7801 cattggcacc agcgaagtgg aaatggtgct tgccacacaa tgtatccttc aaaaccgtcc
     7861 gaaaacaatg cgtatcactt tcaatggaaa gttgggaaaa aacgtactgg ccaaagacct
     7921 cgcgctgtat atgatttcaa aacttacaac cggtggtgca acgggttatt tcgtggaata
     7981 tgcgggagaa gccatccgta acctgagcat ggaagaacgt atgacagtat gcaacctatc
     8041 tattgaaatg ggtgcacgcg gcggactgat tgcccccgac gagactacct tcaactatgt
     8101 aaaaggccgt gaatttgctc ccaaaggcga agaatgggat aaaaaactgg aattctggaa
     8161 aacgttgaag acggatgatg atgctgtatt tgacagtgaa ctgatatttg atgcttccga
     8221 aatagaacct atggtcacct acggcaccaa tccgggaatg ggcatgggaa taagcggaaa
     8281 aatacccaca ctggatgaaa tagatgaagc cggacgtata tcatatatga aatcgcttga
     8341 ctatatgggt ttcaaaccgg gagagaaaat gctgggcaaa cctgtggatt atgtttttct
     8401 gggaagttgt accaacggac gcattgagga tttccgcatt tttgccaact atgtgaaagg
     8461 gaagaaaaaa gcggagaata tcacagcatg gcttgtgccg ggaagctggc aggttgaaaa
     8521 acaaatccgt gaggaaggat tgtatgaaat tcttaccgaa gcaggatttc agcttcgtca
     8581 gcccggttgt tcagcttgtt tggccatgaa cgacgataaa gttccggcag g
//
LOCUS       DAOPYB010000133         8576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9812_length_8576_cov_8.921840, whole genome shotgun sequence.
ACCESSION   DAOPYB010000133 DAOPYB010000000
VERSION     DAOPYB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9812_length_8576_cov_8.921840"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKH58_09755"
     CDS             <1..846
                     /locus_tag="PKH58_09755"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HNX89357.1"
                     /translation="SPVNIKLNLEKNKEYKIRSTTKQAIQQTAGGQQIAIDVLSSNVF
                     SFKVLNQTNDQMDIAFKFDTLASKISSAMFKRETNSAKPGSEPLEKIMNKMSAYKIVA
                     RISTAGKFIQFVNLNKYTDSVLFVLDSIPASKRDDARKQTDALLKESILRSMIEPFFS
                     YLPEKAVNVGESWENSYLNASANMSILTLNTFKLEGVENNLAKLSGTTEAESMPSTDP
                     AAKVTQELKGNLTLQSTIDLTTGLALKNNSKSHFEGVTKMMNGGTPTEIPMKIDGETE
                     TVMFK"
     gene            870..3257
                     /locus_tag="PKH58_09760"
     CDS             870..3257
                     /locus_tag="PKH58_09760"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNX89358.1"
                     /translation="MMRKIFVVLLCLLAINVNARQVITGTLTDKETVKAVNGASVELL
                     MLPDSASVEFTRSSSEGLFILYKADTAKTYCLRVKHLAYRTLVMPVVKKKSSINNLGN
                     IVLEPKTFNLKEVVINGSKVTVTELGDRTVYGIPDGFKKSSADGLDVLRKVPAVQVDY
                     MNEDITVNGKTNIRIEVDGVTRDKGYLKRLHPTQISKMEIITSPSGKYDADVDAVINV
                     ITNPAMRYGLKGMAYGGYFPFSPQSQMGMLNGSLDYGFEKISYYISANGIVQDLGINS
                     TMIRQTNGTQINQNSTIGVKALMGNANVGLIYDPDELNDINLNIAYNNSSNQSNVNAW
                     NFNTVNDFRSIFKNVQNSDNRNDGLNTSLFYKHKFKKDTQHGLEAELKYYNSLNNYTT
                     TDFQNIYYNPADTVELSRSPWMRELSDTRVRTLSGQTNYTLPFDSVYFLNVGISGNYN
                     NYRTDNTSPRNNAVDMDYRDLRAGGYAEISRTFKKGSIKAGSRFETSHVIINSAEGGN
                     YFSPLPYANATWKLNDKHNMKLSYSRRVIRPSSSQLNPFVSVVDSQTISHGNINLKPA
                     YRDNFQFTYNLRLGIKKVTLNISPQFFYEYKTGLIQNILSLNSETKLFESVPTNISNG
                     YETGSALSVNSQIGKVLFNSNFRYSFYHIDQYKDQINSTNRHGWNWNSFILFPLPHDF
                     QIMSVMNFNGPVLDGQTETKSSPFYMMGAGKQFKGNHTVRLLVFNPFLGKFFENTSTI
                     KNATVYQRSDYYMKSNFSFMLMYAYSFKVGKTIEKEKHNVEQQLQDNVIKMPMSF"
     gene            3458..5713
                     /locus_tag="PKH58_09765"
     CDS             3458..5713
                     /locus_tag="PKH58_09765"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNX89359.1"
                     /translation="MVLDIDMIRDVYATLPEKVNQAKNILQRPLTLTEKILFAHLSPG
                     VPVNDYIRATDYIDFAPDRVAMQDATAQMALLQLMNSGRTTVAVPSTVHCDHLIQAHI
                     NAASDLQTASKANNEVYRFLSDVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTPNAGGLGMIAIGVGGADAVDVMAGLPWELKMPKIIGVKLTGKLSGWTSPKDVILK
                     LAGILTVKGGTNAIIEYFGEGTASLSATGKATICNMGAEVGATTSIFPFDEKSAAYLE
                     GTGRKEVADEARKIITCLQPDAEIAVSPEKYYDQVIEINLSELEPYINGPFTPDAAYK
                     VSEFAKIVKEKGYPQKMEAGLIGSCTNSSYEDLTRAASVVKKAKEDGLTVNAQFIINP
                     GSEQVRYTAERDGVLGEFESVGAVIMANACGPCIGQWKRDDDTPNRPNSIVTSFNRNF
                     AKRNDGNPNTHAFVASPELVAALTIAGDLCFNPMKDTLNNEKGEAVKLAEPSGYEMPP
                     KGYEVKDAGYQAPLADGNKVKITIDPQSARLQKLIPFPAWDGKDYLELPLLIKAQGKC
                     TTDHISMAGPWLRFRGHLDNISDNMLIGAVNAFNEKSNAVLDTDTGEYMTVPALARKY
                     KAAGLGSVVVAEENYGEGSSREHAAMEPRFLNVKAILVKSFARIHETNLKKQGMLALT
                     FDNKDDYKKVREDDRISITGLTEFKPGKNLTVILNHTDGTSDKFEVRHTYNYQQIGWF
                     KAGSALNSMKK"
     gene            5720..6985
                     /gene="icd"
                     /locus_tag="PKH58_09770"
     CDS             5720..6985
                     /gene="icd"
                     /locus_tag="PKH58_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNX89360.1"
                     /translation="MSNQNIIKQGEQLIVPDKPVIPFIEGDGIGKEIWTSVKKITDSA
                     IENAYNGKRKINWLEVLAGEKAFNKTGTWLPTETMEAFRNYLVGIKGPLTTPVGEGIR
                     SLNVALRQELDLYVCLRPVRWFKGVTTPLLQPEKVHVTIFRENTEDIYAGIEWNAGTP
                     EVEKVLRFLKEEMGVAKIRFPETTSIGVKPVSSEGTERLVRAAIKYAIDNKLPSVTLV
                     HKGNIMKFTEGGFKKWGYALAEREFDDKVFTAEKFKKIENEQGVDAAVKYYDDAISKG
                     KVFIKDVIADAFLQNTLLKPEEYSVIATLNLNGDYISDQLAAMVGGIGIAPGGNINYK
                     TGHAIFEATHGTAPDIAGQNKANPCSMLLSAIMMLEYLGWNEAGELITRALEDMFQNG
                     IATNDLARFMKNGISLSTTEFSEELIRRL"
     gene            7115..8470
                     /locus_tag="PKH58_09775"
     CDS             7115..8470
                     /locus_tag="PKH58_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNX89361.1"
                     /translation="MDNKHLIYKLSESIKKTSKIDKELFTKLGVKRGLRNEDHSGVLA
                     GLTRVGDVVGYEKQEDGKLKAIPGKLFYRGIDVEDLVHGLHDEDRLGFEETAFLLLSG
                     QLPDKEELDAFKNVLTSTMPLEHKAMMSILSLQGKNIMNILARSVLELYTYDEDPDAI
                     TKDNLISQSINLIAKFPTIIAYAYQVLRHSQHGRSLHIRYPDERMSVAENFLYMMKGP
                     GRYTQLDVKILDLALILHADHGGGNNSTFSVRVTSSTETDTYSAIAAGIGSLKGPLHG
                     GANLMVNAMLDDMKANIKNWKDQKEVDAYLIKILNKEVFDKTGLIYGIGHAVYTVSDP
                     RAGLLKEMAHELAKEKGLEKEFAFMELIEDRAVKVFMNYKGEGTKQTCINVDYYSGFV
                     YEAIGLPKEVFTPLFAMARIVGWTAHRIEELNFSSKRIIRPAYKNVREKQPFVPMSQR
                     D"
BASE COUNT         2765 a         1835 c         1807 g         2169 t
ORIGIN      
        1 tcgcctgtaa atattaaact taacctcgaa aaaaacaaag agtacaaaat cagaagtaca
       61 accaaacaag ctattcagca aaccgctggc ggtcagcaga ttgctatcga tgttctatca
      121 agtaatgtat tcagtttcaa ggtccttaat cagacaaatg accagatgga cattgccttt
      181 aaatttgaca cactggcttc caaaatcagt tcggccatgt tcaaacgtga aactaattcg
      241 gccaaacccg gcagcgaacc tctcgaaaaa atcatgaata aaatgagtgc ttataaaatt
      301 gtcgcaagaa tttctacagc tggaaaattc atccagtttg tcaacctgaa caagtacacc
      361 gacagtgttc tttttgtgtt ggactctatt cctgcatcaa aacgcgatga tgcccgcaaa
      421 caaaccgatg cactgctcaa agaatcaatt ctcagaagca tgattgaacc tttcttctca
      481 tacctgcccg aaaaagctgt aaatgttggc gaaagctggg aaaattccta cctgaacgca
      541 tccgccaaca tgtcgatact cacactgaac accttcaagc tggaaggagt tgaaaacaac
      601 ctggctaaac tgtcgggtac caccgaagcc gaatcgatgc cttcgaccga tcccgcagcc
      661 aaagtaaccc aggagctgaa aggtaatctg accctacaat ctacaataga ccttacaacc
      721 ggacttgcac ttaaaaataa ctcgaaaagc catttcgaag gagtaacaaa gatgatgaac
      781 gggggaactc cgactgaaat accaatgaaa attgatggcg aaacagaaac ggtaatgttt
      841 aaataaaaaa gcgcaaaaaa accgtaaaaa tgatgcgaaa aatttttgtt gttttacttt
      901 gcctgcttgc aatcaacgtc aatgcacgac aggtaataac cggcacgctt actgacaagg
      961 agacggtaaa agctgtgaat ggcgcaagtg tggagttact catgctaccc gacagtgcat
     1021 cagtcgaatt caccagatcc agttccgagg gactttttat cctttacaaa gccgatacgg
     1081 caaaaaccta ttgtttgcgg gtgaaacacc tggcttacag aacactggtt atgcccgttg
     1141 tgaaaaagaa aagttccatc aacaacctgg gcaacatcgt actggaaccc aaaacattca
     1201 acctcaagga agtagtgata aacggctcca aagtaactgt aacagaactg ggcgaccgca
     1261 cagtgtatgg catccccgat ggtttcaaaa aatcatcagc cgacggactg gatgtactgc
     1321 ggaaagtacc ggctgtgcag gtggactata tgaacgaaga cattactgta aacggaaaaa
     1381 caaatatacg gatagaagtg gatggagtta cacgtgataa aggatatctg aaacggcttc
     1441 atcccaccca gataagtaaa atggaaatca tcaccagtcc ttcgggtaaa tatgacgcgg
     1501 atgtggatgc cgtgattaat gtgattacca atccagccat gcgttacgga ctaaaaggaa
     1561 tggcatatgg aggttatttt ccgttcagtc ctcaatcgca gatgggaatg ttgaacggca
     1621 gtctcgacta cggattcgaa aaaatatcct actatatttc agccaacgga atagtccagg
     1681 atctgggtat caattccaca atgatacgtc agacaaacgg aacacaaatt aatcagaaca
     1741 gtactatcgg ggtaaaagcc ctgatgggaa atgccaacgt ggggcttatt tatgacccgg
     1801 atgaactgaa cgatatcaac ctcaacattg cctacaacaa cagttcgaac caatcgaacg
     1861 taaatgcctg gaacttcaat actgtaaatg actttcgtag tattttcaag aatgtgcaga
     1921 attctgacaa cagaaatgac ggactgaata cttcgctgtt ttataagcat aaattcaaaa
     1981 aagacactca gcacggactg gaagccgaat tgaaatacta caacagtctg aacaattaca
     2041 caacaaccga cttccaaaac atctactata atccggccga tactgttgaa ctttcgagaa
     2101 gcccatggat gcgggaactt tccgatactc gggtacgtac gctttccggt cagaccaact
     2161 atacactgcc tttcgacagt gtttacttct taaatgtagg tattagtggc aactataata
     2221 actatcgtac cgacaacaca agtccgcgaa acaatgctgt cgatatggat taccgcgatc
     2281 tgagagccgg tggctatgcc gaaatatccc gaacattcaa aaaggggagc attaaagctg
     2341 gttcaagatt tgaaacttct catgttatta tcaactcagc agagggtggt aactatttct
     2401 caccactacc ctacgccaac gcaacctgga agttgaacga caaacacaac atgaaactgt
     2461 cctactcccg cagagtaatc agaccttcta gttcgcaact caatccgttt gtcagcgtag
     2521 tagatagcca gactatcagt catggcaata tcaatcttaa acctgcttac agagataatt
     2581 ttcagttcac ttataacctg agactcggta taaaaaaggt cacactcaat atttcaccgc
     2641 aatttttcta cgaatacaaa accggtctga tacaaaacat tttaagtctg aacagcgaaa
     2701 caaagctgtt cgaaagtgtt ccgacaaata tctccaacgg atacgaaacc ggaagcgcac
     2761 tttcagtaaa ttctcagatc ggaaaagtat tgtttaattc aaatttcaga tacagttttt
     2821 atcatattga ccagtacaaa gatcagatta attctacaaa tcgtcacggc tggaactgga
     2881 attcatttat cctgtttccc ctaccccacg actttcagat tatgtcggtg atgaacttca
     2941 acgggccggt actcgacgga cagactgaaa ccaaatcatc gccattctat atgatgggag
     3001 ccggtaagca attcaaagga aatcatacag tgaggctttt agttttcaat ccctttctcg
     3061 gaaaattctt tgaaaatact tcaaccatca aaaatgcgac agtctatcag cgcagtgatt
     3121 attacatgaa atcgaacttc tcctttatgc tcatgtatgc ctacagcttc aaagtgggta
     3181 aaaccattga aaaggaaaaa cacaacgtgg aacaacaact gcaggataac gttatcaaaa
     3241 tgcctatgtc gttctgattt ttttgtaggt tttcaggttc atttcatacg gtcagtcagc
     3301 ttacgggttg actgactttt tttgttttaa ttattgtcca tcatttatgt tctgtccatt
     3361 gttaattaaa atttccgaaa cgacctgaac aattcttttg gtcttttgtt agacttttta
     3421 taattaacat ttaacaaacg tataaaattc atgtgatatg gtattagata ttgatatgat
     3481 ccgtgatgta tatgccaccc ttcccgaaaa agtaaatcag gctaaaaaca tcttacaacg
     3541 gccccttaca cttactgaaa agatattatt tgcacatctg tctccgggtg ttcccgtgaa
     3601 tgattacatc cgcgctaccg attatattga ttttgcgccg gatcgggttg ccatgcagga
     3661 tgcaaccgcc cagatggctt tgctccagct gatgaattca ggacgaacca cagttgccgt
     3721 accttctacc gttcattgcg atcacctcat acaggctcat ataaatgccg catccgacct
     3781 gcaaacggcc agtaaggcaa ataatgaagt ttacaggttt ctgagcgatg tatcgaacaa
     3841 gtatggaatc ggtttctgga aacccggtgc gggtattatt catcaggtag tactcgaaaa
     3901 ttatgctttt ccggggggaa tgatgattgg aacggactca cacacaccca atgccggagg
     3961 actgggtatg atagccatag gcgtaggtgg tgccgatgca gtggatgtta tggccggact
     4021 accctgggaa cttaaaatgc ccaaaataat cggggtaaaa cttaccggta aactatcggg
     4081 ctggacatca ccgaaggatg tcattcttaa acttgcaggc attctcaccg taaaaggagg
     4141 aacaaatgcc attatcgagt atttcggcga ggggactgct tcactttcag caaccggaaa
     4201 agcaaccatt tgcaatatgg gtgccgaagt cggcgcaaca acctctattt tccccttcga
     4261 tgaaaaatcg gcagcttatc tggaaggcac cggacgtaag gaagtggccg atgaagcacg
     4321 aaaaattatc acctgtttgc aaccggatgc tgaaattgct gtcagtccag aaaaatacta
     4381 cgaccaggtg atagaaatca atctgtcgga acttgaaccc tatatcaacg ggccatttac
     4441 acccgatgca gcctataaag tttctgaatt tgccaaaatt gtaaaagaaa aaggctatcc
     4501 gcaaaaaatg gaagcaggac tgattggctc ctgtaccaac tcgtcgtacg aagaccttac
     4561 acgagctgca tctgttgtga agaaagccaa agaggatgga ctgacagtta atgctcagtt
     4621 tattatcaat ccgggatctg aacaggtacg ctataccgcc gaacgggacg gtgtactggg
     4681 cgagtttgaa agtgtcgggg ctgttatcat ggccaatgcc tgcggaccct gtatcgggca
     4741 atggaaacgc gatgacgaca ctcccaatcg tccgaattca attgtcacct cattcaaccg
     4801 taactttgcg aagcgtaatg acggaaatcc gaatacccat gcttttgtag cttcaccgga
     4861 attggtagca gctctgacta ttgccggcga tttatgtttc aatccgatga aagatacctt
     4921 gaacaatgaa aaaggcgaag cggttaaact ggctgaaccg agtggttatg aaatgcctcc
     4981 gaaaggctat gaagtaaaag atgccggtta tcaggctcca ttggctgacg gcaacaaagt
     5041 gaaaattacc attgatccgc aatctgcacg tcttcagaaa ctcataccat ttcctgcatg
     5101 ggacggaaaa gactatctgg aacttccgtt gcttattaag gctcagggta aatgcaccac
     5161 cgaccatatt tctatggcag gaccctggct gcgtttccgc ggtcatctcg ataacatctc
     5221 ggataatatg ctcatcggtg ctgttaatgc tttcaacgaa aaatcaaacg ctgtgctcga
     5281 taccgatacc ggcgaatata tgactgttcc ggcacttgcc cgcaaataca aggctgccgg
     5341 gctgggctca gtggttgtgg cagaagaaaa ttacggagaa ggttcgagcc gcgaacatgc
     5401 cgccatggaa ccgcgtttcc tcaacgtgaa agctatcctg gtaaaatctt ttgcccgcat
     5461 tcacgaaacg aacctgaaaa aacagggaat gctggctctg actttcgata acaaagacga
     5521 ttataagaaa gtccgggaag atgaccgaat cagtattacc ggactgacag agttcaaacc
     5581 agggaaaaac ctgactgtaa tactaaatca tactgacggt acaagtgata aatttgaagt
     5641 acgccacacc tacaactatc aacagatcgg atggttcaaa gccggaagtg cactgaattc
     5701 aatgaaaaaa tagatattca tgtcaaatca aaatataatc aaacaaggcg aacaactaat
     5761 tgttccagac aaacccgtca ttccttttat cgaaggtgat ggcatcggta aggaaatatg
     5821 gacctcggta aaaaagatta ccgacagtgc tattgaaaat gcatacaatg ggaaacgtaa
     5881 aataaactgg ctggaagtac tggcaggtga aaaagcgttt aataaaaccg gaacctggct
     5941 accgactgaa accatggagg cttttcgtaa ctacctggtc ggtattaaag gaccacttac
     6001 aactccagtt ggtgaaggca tccgttccct gaatgtagct ttgcgacagg aactggattt
     6061 atatgtctgc ctgcgtcctg tacgctggtt caaaggcgta acaactcccc tgctccagcc
     6121 cgaaaaagtt catgttacaa tctttcgcga aaataccgaa gatatatacg ccggtatcga
     6181 atggaatgcg ggaacaccgg aagtggaaaa agttcttcgt tttctgaaag aagaaatggg
     6241 agtagctaaa atccgttttc cggaaacaac ttccattggt gtgaaaccag tttccagcga
     6301 aggtacggaa cggcttgtac gtgccgctat taaatatgcc attgacaata agttgccttc
     6361 tgtgacactt gtacacaaag gtaatataat gaaattcacc gaaggaggtt tcaaaaaatg
     6421 gggttatgcc ttggctgaac gcgaattcga cgataaggtt ttcactgccg aaaaattcaa
     6481 aaaaatagaa aatgaacaag gtgtggatgc ggcagtaaaa tactacgacg atgcaatcag
     6541 caaaggtaaa gtattcatca aggatgttat tgctgatgca ttccttcaaa atacgctgct
     6601 taaacccgaa gagtactcgg taatcgccac tctcaatctg aacggtgact atatttcaga
     6661 tcagctggct gctatggtag gcggaattgg aatcgcaccc ggaggcaata tcaactacaa
     6721 aacaggacat gccatctttg aagcaactca cggaactgcc cccgacattg ccggacaaaa
     6781 caaggctaat ccgtgttcca tgctactttc tgctattatg atgctggagt accttggatg
     6841 gaatgaagca ggcgaactga taacccgggc acttgaagac atgttccaaa atggcatagc
     6901 tacaaacgac cttgcacgct ttatgaaaaa tggcatttcg ttgagtacaa ccgaattttc
     6961 ggaagaactg ataagaagac tttaactgtg aaaattctta cgtgaaaaat tttattaaaa
     7021 aaaatctatt tatcatagaa caatatcact aattaaaaca ttattaatgt aatttggata
     7081 ataaaatgaa attaacaata taaattttac tcacatggac aataagcatt tgatttataa
     7141 gctgtcagag tcgataaaaa aaaccagcaa aatcgacaag gaacttttca ccaaactggg
     7201 tgtgaaacgc ggacttcgta atgaagatca ttctggagta ttggccggat taacccgtgt
     7261 aggcgatgtt gtcggctatg aaaagcagga agacggtaaa cttaaagcaa tacccggcaa
     7321 actattttac cggggaattg atgtggaaga tttggtacat ggacttcatg acgaagaccg
     7381 tttgggattt gaagaaactg cttttttgtt gctcagtgga caacttcccg acaaggaaga
     7441 gctggatgct ttcaaaaatg tactgacttc gaccatgcca ctggaacaca aagccatgat
     7501 gagtattctg agtttacagg gtaagaatat tatgaatatt ctggcacgta gtgttctcga
     7561 actttacacc tatgatgaag atccggatgc tataacaaaa gataacctga tcagtcagtc
     7621 tatcaatctg atagctaagt ttccaactat tattgcttat gcctatcagg tactgagaca
     7681 ttcacaacac ggacgctcgt tacatatccg ttatccggat gaaagaatgt cggttgctga
     7741 gaatttccta tatatgatga aaggtccggg cagatacact cagttggatg taaaaatcct
     7801 tgacctggca cttattctcc atgcagacca tggtggaggt aacaactcta ccttctcagt
     7861 acgggtaaca agttctaccg aaacagatac ctattccgct atagcagccg gtatcggttc
     7921 tctgaaagga ccgcttcacg ggggggcaaa tctgatggtt aacgccatgc ttgatgatat
     7981 gaaagcaaac attaaaaact ggaaagatca gaaagaagtg gacgcctatc tcattaagat
     8041 tctgaataaa gaagtatttg acaaaaccgg attgatatat ggtataggtc atgccgttta
     8101 caccgtaagc gacccacggg ccggactact caaggaaatg gcgcatgaac ttgctaaaga
     8161 aaaaggactt gaaaaggaat ttgctttcat ggaactaatt gaagaccgtg ctgttaaagt
     8221 atttatgaat tataagggtg aaggaacaaa acaaacctgc atcaatgttg attattattc
     8281 gggatttgtt tatgaagcta ttggtcttcc aaaagaagtc ttcactccgc ttttcgctat
     8341 ggcccgtatc gttggctgga cagctcaccg tatagaagaa ctcaacttca gcagcaaacg
     8401 tatcatccga cctgcttata aaaatgtgcg tgaaaagcaa ccctttgttc caatgtctca
     8461 aagagattga tttaatttga ataataccaa tctccggtca aagagtgaat tagtccacac
     8521 tgaccggagt ttgttttttt caccacatgt taatctattt attcagaaaa aaacat
//
LOCUS       DAOPYB010000134         8497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_9947_length_8497_cov_11.919924, whole genome shotgun sequence.
ACCESSION   DAOPYB010000134 DAOPYB010000000
VERSION     DAOPYB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9947_length_8497_cov_11.919924"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..530)
                     /locus_tag="PKH58_09780"
     CDS             complement(318..530)
                     /locus_tag="PKH58_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89362.1"
                     /translation="MSEPTLGAAVILRLTERLEVCDAVSADYLKQIRRIGTFAASDNG
                     FGVDSAGQFHNCAEFVLAVHLLKFLL"
     gene            571..1359
                     /locus_tag="PKH58_09785"
     CDS             571..1359
                     /locus_tag="PKH58_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX89363.1"
                     /translation="MTNEELLQQIRNEEGDDHTLATLWEQVCRLFYMHSDGLYHRHKG
                     RADSCGVERDDCRQVCWFAFLDAIKAYNGKPDNELMFSTYAKYHVRRRVYELLGYRTS
                     KREPLNGAVSLDAPLPGNEDEKITIGDTIADSSAEAPFEDIDCSDTAAEVLERVSALP
                     TKQAAAVRGRFWQDKTLTEVGAELGLTPKQIHNQYQAAIGKLRRDKRLQEIRDDYYAN
                     TTLTKHTGFQFFKENGMSSVEWHLLKLEERLERAKGGDERDGVQ"
     gene            1512..1823
                     /locus_tag="PKH58_09790"
     CDS             1512..1823
                     /locus_tag="PKH58_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89364.1"
                     /translation="MRNYPLIKMNKEGTLLRPQHSYYSDEYTENMCDLFLRDCLVKDE
                     QGKQHQYYRLHARQPHNIEMAFAYDIQCPECNSGMLKQISRPLNYNELGLYRCPVCDK
                     K"
     gene            1835..2776
                     /locus_tag="PKH58_09795"
     CDS             1835..2776
                     /locus_tag="PKH58_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNX89365.1"
                     /translation="MSTYTERLSYTGSPEYDKHFWNAMRGNVGSFDEIAKGRSTGIGL
                     YAMPNSAQNKYMAALEKESLLRSIGTVIKAYGSGYRIFAKDSNDLAQWVAENDAVPIY
                     DGIDDFNINTVDSWNLAALVKMDAAFIHDASFDIENYLIKRFAKNFGRAEDNAFINGN
                     GNEMPTGILNATGGAEVAATIDDISYDDVISLYFSVKPEYRKNAVWLMNDKTALALRT
                     LKDNTGNYLWRDSDDTILGKKVIMSEFMPDADAGAKPIAFGDFSYYWVIGRSPIGVRT
                     LSEKFAVLDQIGYLAYEFLDGKLVRSEAVKVIRINTP"
     gene            complement(3307..3888)
                     /locus_tag="PKH58_09800"
     CDS             complement(3307..3888)
                     /locus_tag="PKH58_09800"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX89366.1"
                     /translation="MEKKKIDQRVRLTQHLLKNALVQLMQEQHISKISIRAICEFAGI
                     NRSTFYVHYTDQYDLLKKVEQEVLDNLNKYLGKQDLSDERPISIQILIKVLDYVKENM
                     ELFKALLSENCDFAFQKDLVALAQIISSQQNQLFDLRTQEYIKIFGFTGTISMLQKWL
                     NDGANESPAQMAEFIIQIVYYGISGLKKNTFNP"
     gene            4039..6099
                     /locus_tag="PKH58_09805"
     CDS             4039..6099
                     /locus_tag="PKH58_09805"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNX89367.1"
                     /translation="MDRFSNIIIRHKKPVIVIFIAVALVCLFLLLFVDVNYNMVDYLP
                     PDAQSTTALNIMNSEFTESMPNASVMAKNVSLTEALEYKQKLASIDGVTQVMWLDDMM
                     DIKQPLEMGDTDTIEGFYKDGNALFSVTIAKGIEKEACNDILNLIGSDNALTGEAPAL
                     VFIQETTASTVVKALAILLPVVILILVLSTASWLEPLLFLLVIGISIVINMGTNIIFG
                     EISFMTNSISPILQLACSLDYAVFLLHSFQDNRKKYTNVEDAMRHAMKESMSTVAASA
                     ATTLFGFLALLFMNFGIGADLGINLAKGIIFSFISVMVFLPALILCIYKMIDKTQHRA
                     FMPSFGNVNRVLSKIAVPAVIIVSILIVPTFLGQGRTGFLYGNDTVDPNTRYGRDTVA
                     VEQQFGKSTVMALLVPRGDVAKEQMLCDELEQLDRITGVMSYAANVGAVIPPEYLGDD
                     ITGQFYSENYARIIVYTDTPAEGDIAFKTVENINEKTKAHYGETFYTLGQSANLYDMK
                     SIVQKDNVVVNLIAVIAIFIVLLVTFKSATLPFLLVLTIEAGIWINLAIPYFSGTPIN
                     FLGYLVVSTIQLGATVDYAILLTNTYLRNRRQMPKREAISVSLGGSFKSILVSALTLS
                     TAGFTLFATSSNSAIADIGLLLGRGTLLSFAMVVCFLPTLLVVFDKAIEKVTFKTKFF
                     HQSK"
     gene            6127..7986
                     /locus_tag="PKH58_09810"
     CDS             6127..7986
                     /locus_tag="PKH58_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89368.1"
                     /translation="MMKSKIFKTILSISLAAVLSLTPASLSLAASGNAVSGPAVSASG
                     NAEIKGKDEVIYAILAPDGSIRAMYAVNHFAIAEAGSITDYGDYTSVKNLTDTTLMTQ
                     NGDAVTFQTNAENFYYQGNMTTTELPWIFDISYFLNGVKTAPQELAGKSGKIEIHITT
                     QKNTAVNSTFNENYMLQISITLDTEKCSDINAAGATVASAGKNKLIAYTVMPGKDADI
                     SLSATARDFAMTGIDISAVPFSMNMEMPDTDSMISDFTELSDAISDLNDGVGDLADGV
                     AELKTGSDKLRNGSSDIKAGLSELSDNSEQLLQASSQISGALTQIASSLNGASGEMDL
                     GDLTQLPQGLSQLADGLEGISDGLIELKNGFTSAYAALDGAIQGIPDATVSQEQITEL
                     YTQTDPSQYALLDQLIASYTAGQTVKGTYNQVKGAFDAVGSTIDTLAASIDTISATLD
                     DMSEKISDALSGMDMMEQLAQLSAGLSELDRNYAAFHSGLRNYMNGVGALSSGYADFH
                     NGVSSFRNGIGELYDGVAELHDGTTELSDETAKMPDKIQSEIDDMLDQYTGLDFEPIS
                     FTSSKNEHTDLVQFVLKCDGIEKPEETKGVEVEVKNETFWDRLISLFTGGKKE"
     gene            complement(8000..8191)
                     /locus_tag="PKH58_09815"
     CDS             complement(8000..8191)
                     /locus_tag="PKH58_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89369.1"
                     /translation="MSDIYSTAKRGNKTQEGKDYVTTFQRNRRVAQSPCSSNQRDNRQ
                     LQPTWQQLPYRNKVYATYL"
BASE COUNT         2334 a         1924 c         2009 g         2230 t
ORIGIN      
        1 agaacattta aggagggaaa ggaatggtac tgggacatac ccgaagcggc aaaggagaat
       61 atcaaggagg acaatattcc ctaagggtgt atttatggca ctcttcacgg ccaagaagtc
      121 aagaatgcca taaaccacaa ttaaggaatt attggcactc atgaaacgaa aaaagaccat
      181 aagcctgtgt ttatcgatgt ttccgaggtt ggagcaacga aagaaccaag tcaagggtgc
      241 caaaattccc aaagctacga agagtgccat caagggtgcc aatattcaat cgaaaagagg
      301 gaagccgaat gccggactta cagcagaaac ttgagcaaat gtacggcgag cacaaattcc
      361 gcacaattat ggaactgccc cgcgctgtca acgccaaagc cgttatccga tgcagcgaaa
      421 gtgccgatgc gccgtatctg tttcaggtag tcagccgaaa cagcgtcaca tacttcaaga
      481 cgttccgtca ggcgcaggat tactgccgca ccaagggttg gctctgacac cagcattata
      541 aacaccatca cagaaggagg tggaccgaca atgactaacg aggaattatt acagcaaatt
      601 cgaaacgagg agggcgatga ccatactctg gctacgctct gggaacaagt ctgtcgcctg
      661 ttctatatgc actctgatgg attatatcac cgccataaag gccgtgcgga ttcttgcggc
      721 gtggagcgtg atgactgccg tcaagtctgc tggttcgctt tccttgacgc aatcaaggct
      781 tacaacggaa aacccgataa cgaattgatg ttctcgacat acgccaaata tcacgtcagg
      841 cgcagggtat atgaactgct cggttatcgc acaagcaagc gtgagccgct gaatggtgcg
      901 gtcagtcttg acgctccgct ccccggcaat gaggacgaaa aaatcaccat cggcgatacc
      961 atagcagact catcagcgga agcaccgttt gaggatatcg actgctcgga taccgccgcc
     1021 gaggtgctgg aacgcgtatc cgctctgccg acaaagcaag cggcggctgt tcgagggcgc
     1081 ttctggcagg ataaaacgct caccgaggtc ggagcagaat tagggctaac tccgaaacag
     1141 atacacaacc agtaccaagc ggctatcgga aaactgcgcc gtgataagcg gcttcaggaa
     1201 atccgcgacg actactatgc caacacgaca ttgacaaagc acacgggctt ccaattcttc
     1261 aaggaaaacg gtatgtcctc ggtggaatgg cacttgctga aactggaaga acggctcgaa
     1321 cgtgcgaaag gaggtgatga gcgtgacggg gtgcagtaag cggacaatga ctatccgcta
     1381 tcagaatttg ccgctcccgc agaacaaaca cggcaagcgc ccgtggtggg aggagcggag
     1441 ccgaaccaaa gccgcagaag cggcgaaccg taggaaagca atcaaacact aatttttagg
     1501 aggaacttca aatgagaaac tatccgctta tcaaaatgaa caaggagggc actctccttc
     1561 gtccgcaaca ctcatattac tctgatgaat acacagaaaa tatgtgtgat ttattcctga
     1621 gagattgtct tgttaaagac gagcaaggaa aacagcatca gtattatcga ctccatgcgc
     1681 ggcagcctca caacatcgaa atggcttttg cttacgatat ccagtgcccg gaatgtaata
     1741 gcggtatgtt aaagcaaatc agcagaccgc tcaattataa cgaactgggt ctatacagat
     1801 gccctgtttg tgacaagaaa taaggaggga aattatgagt acttacactg aaagattatc
     1861 ctataccggc tcgccggaat atgacaagca tttctggaac gctatgcgcg gtaacgtcgg
     1921 cagttttgac gagattgcaa aaggccgcag caccggaatc ggtctttatg caatgccaaa
     1981 cagtgcgcaa aataagtaca tggcagccct tgagaaagaa agcctgctcc gtagtatcgg
     2041 tactgtaata aaggcttacg gctccggcta ccggattttc gccaaggaca gtaatgacct
     2101 tgcccagtgg gtagcggaaa atgacgcagt tcctatttat gatggcattg acgatttcaa
     2161 catcaacacc gtggacagct ggaacttggc ggctttagta aaaatggatg cggcctttat
     2221 acatgacgcc agttttgaca ttgagaacta cctcatcaaa cgcttcgcca agaacttcgg
     2281 tagggccgag gacaacgcct ttatcaacgg caacggtaat gagatgccca caggcatcct
     2341 gaacgccacc ggcggagcgg aagttgctgc taccatcgat gacatcagct acgatgatgt
     2401 tatcagcttg tacttctcgg taaagccgga gtaccgtaaa aacgctgtct ggctgatgaa
     2461 cgataaaacc gctcttgctc tgcgcaccct taaggacaat accgggaatt acctctggcg
     2521 tgacagcgat gataccatct tgggcaagaa agtaataatg tccgagttta tgccggatgc
     2581 ggatgccggt gcaaagccta ttgctttcgg tgactttagc tattactggg ttattggaag
     2641 gagtcccatc ggcgttcgca ctttatccga aaagttcgct gtcctcgacc agattggcta
     2701 ccttgcctat gagtttcttg atggcaaact tgtccgcagt gaagccgtaa aggttattcg
     2761 gattaatacc ccttaaccga gtggcacaag cacataagta acaatgctgg gagagttgat
     2821 ataggctctc ccggtctttt tattatgtgc aattacgctt tttgccgtat aattacatgg
     2881 ctttgcaaag cgttttccta aaattaggct attaccccgt ttgaatccgc gtttttgtgt
     2941 acgaagcccc acgccgctgt ccgagttgaa aagctgtaga gaacgaaacg cccccacggt
     3001 atgattttga tttcaaagtt tttgattttt gatttttgaa aaactcatgc gaaaggccaa
     3061 gcgcgctgtc ctaatcaaaa gtggtaggga tgtagaccgc ccctaccgcc gggcgtattt
     3121 cttgctcgca ctattgccac tcgctatagc ttgctttttc attgtttatg ggtaaaattc
     3181 atcttttaag agacggcatt ttggccggaa atttgaacca ttatcgtgca atcatgcatc
     3241 ccctgcctgg ggttgttgtt gggaggtgca ttttttattt ggggtgtgca cttttacacg
     3301 tttttttcaa ggattaaatg tgttcttttt taaaccgctt attccgtaat aaactatctg
     3361 tataataaac tctgccatct gcgccggtga ttcattcgca ccgtcattca accatttttg
     3421 cagcatactg attgtgccag taaagccaaa tatcttaata tattcctgtg ttctcaagtc
     3481 aaacaattgg ttttgctgcg atgatataat ttgcgccaac gcaactaagt ctttttggaa
     3541 ggcaaagtca cagttttcgc taagcagcgc cttaaacaac tccatatttt ctttcacata
     3601 gtctaaaact tttattaaaa tctgaatgga tatggggcgc tcatcactta aatcttgttt
     3661 gccgagatat ttattcaaat tgtccaggac ttcctgttca acctttttca gaaggtcata
     3721 ttgatcggta taatgcacat aaaaagtgct gcgattaatt cccgcaaatt cacatatcgc
     3781 cctgatagaa attttggata tatgctgctc ttgcatcagt tgcaccaaag cgtttttcag
     3841 aaggtgttgt gtcagcctga ctcgctgatc tatttttttc ttttccacgt tttcacctca
     3901 ttctccgtaa tccgtcactt ttgatatcac ctgtcggaca tcaaaccatt ttacttaaca
     3961 ttagtggttg taaccataca ctaagcatat tataatacaa tatggtatgg tttgcaatac
     4021 gcgattaggg ggattcttat ggatcgattt tcaaatatca ttattcggca caaaaaaccg
     4081 gttattgtca tattcattgc agtcgcgctg gtgtgtttat ttcttctgct ttttgtagat
     4141 gtcaattata acatggttga ctacctgcct ccggacgcac aatccaccac ggcgctcaat
     4201 atcatgaaca gtgaattcac agaatctatg ccgaatgcaa gcgttatggc taaaaacgtc
     4261 tctcttacgg aagcgttgga atataagcaa aagctggcct ccatcgacgg cgttacgcag
     4321 gttatgtggc tggatgatat gatggacatc aaacagcctc ttgaaatggg cgatacggat
     4381 acgatcgagg gcttctataa agacggcaat gctttgttct cggtcacaat cgccaaaggc
     4441 atcgagaaag aggcgtgcaa cgatatattg aatctgatcg gcagcgataa cgcgcttaca
     4501 ggcgaagccc ctgccttagt atttatccag gaaacaaccg catcaaccgt tgtaaaagca
     4561 ttggcgattt tattgcccgt cgttattctc atattggtat tgtcaacggc gtcatggctt
     4621 gagccgttgc tgtttctcct tgtgatcggt atctccatcg tcatcaatat gggaaccaat
     4681 attatctttg gcgaaatctc ttttatgaca aattcgataa gtcccatttt acaattggcc
     4741 tgttcgttgg actacgccgt tttcctgctt cacagtttcc aagacaacag aaaaaaatac
     4801 accaatgtag aagacgccat gcggcatgcc atgaaagagt ccatgtcaac cgtggcggcc
     4861 agcgcggcaa ccaccctgtt tggtttttta gcccttttat ttatgaactt cggaatagga
     4921 gccgatttgg gcatcaatct ggcgaagggc attatcttca gctttatttc agttatggtt
     4981 ttccttccgg cactcatcct ctgcatttac aagatgattg ataaaacaca acaccgggca
     5041 tttatgccca gctttggaaa cgtcaacagg gttttatcga aaatcgctgt cccggcggtt
     5101 attatcgttt ctattttgat agtaccaacc tttttagggc aaggccgcac cggctttctt
     5161 tatggaaacg atacggtaga ccccaatacc cgttatggca gagatacggt cgcggttgag
     5221 cagcaatttg gcaaatcaac cgtcatggca ttgctggttc cccgaggtga tgtagccaaa
     5281 gaacagatgc tatgcgatga actcgaacag cttgaccgta taacaggcgt aatgtcatac
     5341 gccgcaaacg taggcgcggt catcccccct gaataccttg gcgacgacat aacgggacag
     5401 ttttactcag aaaactacgc ccgcattatc gtatatacgg atacgcctgc ggaaggcgat
     5461 attgcgttca aaaccgtaga gaatatcaat gaaaaaacga aagcacatta tggagagaca
     5521 ttttacacac tcggccaaag cgccaattta tatgacatga aaagtattgt tcaaaaagat
     5581 aacgtagttg ttaatctgat tgccgttatc gctattttta ttgttttgtt ggtcacattc
     5641 aagtcggcaa cattaccgtt tttgttggtg cttaccatcg aagcgggtat ctggatcaat
     5701 ctggccattc cctatttcag cggcactccg attaattttt tgggttactt agtggtgagc
     5761 acgatacaac tgggcgcaac ggtagactat gccatcttgc tgacaaacac ttatttgaga
     5821 aaccgaagac aaatgccaaa gcgagaggcg atcagcgtgt ctcttggcgg gtctttcaaa
     5881 tccatactcg tttcggcgct aaccctttct acggccggat ttacgctgtt tgcaacgtca
     5941 tcgaacagcg ctattgccga tataggtctg ttgctcggac ggggtacgct gctctccttt
     6001 gccatggtgg tatgctttct gcccaccttg ctggtcgtgt ttgataaagc gatcgaaaag
     6061 gtcaccttca agacaaaatt ttttcaccaa tctaaataga acattgaaga ttactgggag
     6121 gtatatatga tgaaatctaa aatattcaag accattctgt ccattagctt ggccgcagtg
     6181 ctgtcgctca ctccggcaag tctgtcgctt gcagcgagcg gaaacgctgt ctccggccct
     6241 gccgtcagcg cctcgggcaa tgcagaaata aagggcaagg acgaggtcat ttacgctatc
     6301 ctcgcgccgg acggcagcat ccgcgccatg tacgccgtta atcattttgc gattgccgag
     6361 gcaggaagca tcacggatta tggcgattat acgtcggtaa aaaatctgac ggatacgact
     6421 ttaatgacgc aaaacggtga tgccgttacc tttcaaacca atgctgaaaa tttctattat
     6481 caggggaaca tgaccacaac ggaactcccg tggatatttg acatatcata ctttctgaac
     6541 ggcgttaaaa cagcaccaca ggagcttgcc ggaaaatccg gtaaaattga gattcatatc
     6601 acgactcaaa agaacaccgc tgtgaattcg acttttaatg aaaactatat gttgcaaatc
     6661 tctataacgc tggataccga gaaatgcagc gacatcaatg cggcgggcgc taccgttgcc
     6721 agcgcgggaa aaaataaatt aatcgcatat acggtaatgc cgggcaagga tgcggatatt
     6781 tccctaagcg caacagccag ggattttgcc atgaccggaa ttgacatttc cgccgtgccc
     6841 ttctcaatga acatggagat gccggataca gacagtatga taagtgattt taccgagctg
     6901 tctgacgcga tctccgatct gaatgatggg gtgggtgatt tagctgatgg tgtagccgaa
     6961 ttaaagactg gctcagacaa attaagaaat ggatcatccg atatcaaagc cggcctttcc
     7021 gagctaagtg acaattccga gcagctttta caggcgtcct cacaaatcag cggtgctttg
     7081 acacagatcg cttcgtctct taacggagcc tccggggaaa tggatttggg tgatctgacc
     7141 cagcttcccc agggcttgtc gcaattagcc gatgggctgg agggcatatc agacggtttg
     7201 atcgagttga agaatggatt tacatcagcc tatgcggcat tggatggagc catacaaggg
     7261 attccggacg caacggtcag tcaggagcaa attacggagc tatataccca aaccgatcca
     7321 agccagtatg ctttacttga ccagcttatc gcgtcgtata cggcaggcca aaccgtcaag
     7381 gggacttaca atcaggtaaa aggcgctttt gacgccgtag gctccacaat agacacgctg
     7441 gccgcttcca ttgatacgat ttcagctacg cttgatgaca tgtcggaaaa aataagcgat
     7501 gcactgtccg gcatggatat gatggagcag cttgcgcaac tttcggcggg gttatcggaa
     7561 ttagatagaa actatgcagc attccatagc gggttaagaa attatatgaa tggcgttggt
     7621 gcgttgtcct ccggttatgc cgattttcat aacggggtgt cttcatttag aaatggtatc
     7681 ggtgagcttt atgacggcgt ggccgagctg catgacggta ccaccgaatt aagtgatgaa
     7741 acggctaaaa tgccggacaa gatacaatcc gaaattgatg atatgttgga tcagtataca
     7801 ggcttggact ttgaaccgat ttctttcacc tcgtccaaaa acgagcatac cgatctggtt
     7861 cagtttgtgt tgaaatgcga cgggattgaa aaacctgaag aaacgaaggg tgtagaagtc
     7921 gaggtcaaaa atgaaacatt ttgggataga ttgatttcgc tgtttacagg tggaaagaaa
     7981 gaataaactt ttccgttgtt tacaggtatg tagcatatac tttgttgcga tatggcagtt
     8041 gttgccatgt aggttgtagt tgcctgttat ctcgctgatt gcttgaacaa gggctttgcg
     8101 ccactcgcct gttacgttga aatgtagtta cataatcctt gccctcctga gttttattcc
     8161 cgcgttttgc ggtactgtat atatcactca tttgagcgtg aattacaagt ggtatccgcg
     8221 aacttgggca tagaattgcc agacggcagg ccgacgaacg aatgtgtgtt atgcacaaat
     8281 ttaaccccgc tgatgaggtg agcagaactc atttcatagg tcggggttcg ttgtttgcag
     8341 tagcgtttcg ttgaatggca tttcgtaaat taaaacacca aataaacacc aggtgccgca
     8401 aaatcctccc tttcccgata gggtaaatcc gacaaagtgc cgtgtttacg ggcttttcag
     8461 gcattatgtt taaaatcgtt gctcaccgtt gtcttta
//
LOCUS       DAOPYB010000135         8451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_10027_length_8451_cov_10.709624, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000135 DAOPYB010000000
VERSION     DAOPYB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10027_length_8451_cov_10.709624"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..540)
                     /locus_tag="PKH58_09820"
     CDS             complement(163..540)
                     /locus_tag="PKH58_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNX89370.1"
                     /translation="MKELTPKEEEIMQFFWKEGALFVKDLVEKYPDPKPHFNTLSTYV
                     RALEEKGFLSHESFGTTYRYFPVISKEENNNRTLKNVIAKHFNNSYLNVVNTFIKSEK
                     ISVDEIRRLLDEVEKNSSQPSQK"
     gene            complement(754..1455)
                     /locus_tag="PKH58_09825"
     CDS             complement(754..1455)
                     /locus_tag="PKH58_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX89371.1"
                     /translation="MSDRALISYKGVEVCQQELVVLKNIDLEINPGEFIYLLGKVGSG
                     KSTLIKSFYHEIPIYEGEARVLDYDLRKIRTKDVAHLRRKIGIVFQDFQLLIDRSVNA
                     NLEFVLRATGWKDKNAIAEQIQHVLKQVGMQTKGYKMPHQLSGGEQQRIVIARALLNS
                     PEIILADEPTGNLDPTTGQDIMELLYNIRRSGTTVLIATHNLSWIDIFEGRVLMCEDD
                     KLLEITAETKQKAEN"
     gene            complement(1504..2097)
                     /gene="hisIE"
                     /locus_tag="PKH58_09830"
     CDS             complement(1504..2097)
                     /gene="hisIE"
                     /locus_tag="PKH58_09830"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNX89372.1"
                     /translation="MIVDFQKMGGLVPAIIQDERTLKVLMLGFMNEEALQKTEETGKV
                     TFFSRTKNRLWTKGEESGNFLEVVSITPDCDNDTLLIKAHPAGPVCHTGADTCWNEEN
                     KTDIAFLQYLQEFIQKRHREMPEGSYTTSLFNKGANRMAQKVGEEAVETVIEAINGTD
                     EGFIYEASDLIYHLIVLLTSKGFSLSDIAAELQKRHK"
     gene            complement(2715..3752)
                     /gene="asnA"
                     /locus_tag="PKH58_09835"
     CDS             complement(2715..3752)
                     /gene="asnA"
                     /locus_tag="PKH58_09835"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HNX89373.1"
                     /translation="MSYFIKPQNYTPILDLQQTELGIQKIKDFFQANLSAELRLRRVT
                     APLFVLRGTGLNDDLNGVERAVNFPIKDMEDARAEVVHSLAKWKRVTLADYNIENGYG
                     IYTDMNAIRADEELGNLHSLYVDQWDWERVMEANERNIDFLKSIVNKIYAAFLRTEYL
                     VSESFPEIRPCLPPEIAFIHSEELCQLYPSLTPKEREYEITKKHKAVFIIGIGGELSD
                     GQRHDGRAPDYDDWTTPGEDGLPGLNGDIVLWNPVLEMPFEVSSMGIRVDKKALQKQL
                     KLSGKEDRLDLYFHKRLMNDTLPLSIGGGIGQSRLCMFFLRKAHIGEIQASIWPEEVI
                     AECKKENIVLI"
     gene            complement(3949..4455)
                     /locus_tag="PKH58_09840"
     CDS             complement(3949..4455)
                     /locus_tag="PKH58_09840"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNX89374.1"
                     /translation="MSKVSIIAAIADNLAIGKNNKLPWHLPADLKHFKQLTTGHAVVM
                     GKRTFESLPNGPLPNRKNIVLTSIPEGDFDKYYEATSIKDALDLCEHEDEVFIIGGAS
                     IFSQAIKMVDNMYITWVHANFEADTFFPKINLKVWKEISREEFNADEQNPYPYTFCQY
                     ERIAPKEK"
     gene            complement(4588..5382)
                     /locus_tag="PKH58_09845"
     CDS             complement(4588..5382)
                     /locus_tag="PKH58_09845"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNX89375.1"
                     /translation="MKQYLDLLDRVLAEGVKKEDRTGTGTISIFGHQMRFNLEEGFPC
                     LTTKKLHLKSIIYELLWFLNGDTNAKFLQDNGVRIWNEWADENGDLGHIYGYQWRSWP
                     DYKGGSIDQITEAVETIKHNPDSRRIIVSAWNVADLPNMNLPPCHAFFQFYVADGRLS
                     LQLYQRSADIFLGVPFNIASYALLLQMMAQVTGLKAGDFVHTLGDAHIYLNHLEQVKL
                     QLTREPRPLPQMLINLNVRDIFSFQYSDFELVNYDPHPHIKGIVAV"
     gene            complement(5409..7403)
                     /locus_tag="PKH58_09850"
     CDS             complement(5409..7403)
                     /locus_tag="PKH58_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX89376.1"
                     /translation="MLKFKSSGLLILLAVITLQSCGIKARLAKADKRYALGEYAVAGD
                     LYKSIYSRIPSKNKPLRAEIAYKQGECYRLISHYRAEQVYLNAVRNKSTNDTVYLRLA
                     QMQQRNGKYAEAAKSYTTYLEKHPDDELAQNGLKATSLAADLKASPTRYLVKKSKEFN
                     VSRSSSFSPVFSSSDADVLIFTSNRKFGKKNVVRKNNGVSGQPNNHLFIARKNNKGKW
                     EATEQLEGDVTSLNDEGIASVSPDGRSMYFTRSLLADDKGGGTIIVLSNRAGGTWSAP
                     QKIQLFRDSTISVAHPAISPDGTTLYFVSDSKDGFGGKDIWRATFEGNEWKYIENLGK
                     DINTPDDEMFPTVREDGTLYYSSKGKPGMGGLDIFEAKPLKEGGWEVKNMGVPINSSA
                     DDFGMAFAGKTESGYFSSNRNESRGYDALWSFELPALEYVLQGKITDPVGETVPDAKI
                     RIIGNNGENARIQAKGDGTYSYKLNKGVDYVMLASARGFLNQKNEISTKNVSDRQSES
                     FKVDFQLTPLYKPVQMNNIFYEFAKWDLTPQSETGLQQLVKLLNDNPNITIEMSAHTD
                     YVGNNESNRILSERRAQSVVDYLIKAGIAKNRLTAVGYGEEQPFTVDISTAKKYNFLK
                     ENTVLTEAFILTLTPVQQEIANQINRRTEFRVLKTTFNLY"
     gene            complement(7484..>8451)
                     /locus_tag="PKH58_09855"
     CDS             complement(7484..>8451)
                     /locus_tag="PKH58_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNX89377.1"
                     /translation="QTMNGRTQYGLVVCASVDDYMTGKIKKHELTRRDKEEDRMKHVR
                     VNNANVEPVFFAYPHRDDLDAIVAGITVNEPEYDFTAELDGFRHQFWVIEDSGIIEQI
                     TRIFAEIPSMYIADGHHRSAAAALVGNEKKLQNPKHRGDEEYNYFMAVCFPDNQLNII
                     DYNRVVKDLNGLSDEEFIEKLKENFIVEEKGAEIYKPDSLHNFALYLSNNWFSLTAKP
                     GTYNDNDPIGVLDVTISSNLILDEILGIKDLRSDKRIDFIGGIRGLGELKRRVDSGEM
                     KVALALYPVSMKQLIDIADTGNIMPPKTTWFEPKLRSGLVIHELV"
BASE COUNT         2078 a         1817 c         1888 g         2668 t
ORIGIN      
        1 acgcccagga gtattgttct gtttgcttta tagaacgtgg ttttgctgat aaatatctta
       61 tagcaaccat aaatcacagc cgccgaaatg ctgatacgaa gcaggtaaaa caggagtgtt
      121 tccatatcct tttggtaatt gttgttagaa attaaaagaa aattactttt gtgaaggttg
      181 tgaggaattc ttttccactt catccagcaa tctgcgtatc tcatccaccg aaatcttttc
      241 gcttttaata aacgtgttca ctacattgag gtaggagtta ttgaaatgtt tggcgattac
      301 gtttttcagt gtacggttgt tgttttcctc cttactgata accggaaagt aacggtaggt
      361 agttccgaag ctctcatgcg acagaaatcc cttttcttcc agcgcgcgta cataggtcga
      421 tagcgtattg aagtgtggtt tcggatcggg atatttttca accaggtctt ttacaaacaa
      481 agcgccttct ttccagaaaa actgcatgat ctcttcttct ttgggagtta attctttcat
      541 gattcgatac tttaaatttt gatgatgcaa atgtaaacta aattatttag tttacaacta
      601 aaaacaatag tttatcaact aaaatatgta gttaaataat gttatctgaa agttcatgtg
      661 ttgtaaatgc ttgaattaca tatttataaa tcaggcagac cggataagtg tgggcttatc
      721 cggtctgcta aaagttatat tccacgtaat ggattaattt tctgcctttt gttttgtttc
      781 ggcggtaatt tcaagcaatt tatcatcttc acacatcagc actctgcctt cgaatatatc
      841 gatccagcta aggttgtggg tggctatcag tacggtggtg cctgacctcc ggatgttgta
      901 aagcagttcc atgatgtcct gtccggttgt cgggtcgagg ttgccggtgg gttcgtcggc
      961 aagaatgatt tccggggaat tgagcagggc acgcgcaatg acaatacgct gctgttcacc
     1021 tcccgaaagc tggtgaggca ttttgtaacc ttttgtttgc atgcctactt gtttgagtac
     1081 atgctgtatt tgctcggcaa tggcattttt gtctttccaa cccgtggcac gaagtacaaa
     1141 ttccagattg gcatttacac tccggtcaat gagcagttgg aaatcctgaa agacgatacc
     1201 tattttacgt cgcagatgcg ccacatcttt cgtcctgatt tttcgcaggt cgtaatccag
     1261 tacgcgggct tcgccttcat agattggtat ttcgtggtaa aatgatttta tcagggtgct
     1321 ttttccgctt cccactttcc cgagcaggta gataaactca cccggattaa tttccaggtc
     1381 aatattcttc agaaccacca gttcctgttg gcatacttct actcccttat agcttattaa
     1441 tgctctgtct gacatgttat ctcttttttt gttgtaaaaa acgactgtaa taaaccttcg
     1501 gaattattta tgtcttttct gaagttcggc agcaatatcg ctcagcgaaa aacctttgga
     1561 ggtgagcagt acaatcaggt gatagatcag gtcggatgct tcgtagataa atccttcatc
     1621 tgttccgttg atggcttcaa tcacggtttc tacagcttct tcacccactt tctgggccat
     1681 tcggttcgcg cctttgttga aaagggaggt ggtgtacgat ccttccggca tttcgcggtg
     1741 gcgtttttga ataaattcct gcaggtattg taaaaatgca atgtcggttt tgttttcctc
     1801 gttccagcag gtgtctgcgc cggtatggca cacaggtccc gcgggatgtg cttttataag
     1861 caaggtatca ttatcgcaat cgggagtgat ggaaaccact tcaagaaagt ttccgctctc
     1921 ttcgcctttg gtccaaagtc tgttcttggt tctgctgaaa aaagtcactt tcccggtttc
     1981 ttcggttttt tgtaacgctt cttcgttcat aaacccaagc ataagtactt tcagtgtcct
     2041 ttcatcttgt ataatagccg gaacaagtcc acccattttc tggaagtcta caatcatttt
     2101 tgtggtgtat ttaacaagtt attttacaat cagcgaaagt acaaaaaaac ggttaaccgg
     2161 acaataaatg aggtataaaa aatgaaaatc cctgaaagcg cttgcaatgc gccgttcagg
     2221 gattaactag aatattaata atgagatgaa atacaaatca gtcctacaac aaagatttgt
     2281 tgttaagttt cagattgata ttttctatat acccgagtgt tagatgtctt tgatattacc
     2341 aaaataacct ataaatacga actgctgaaa aacataactg taaaatgttt cggtactctg
     2401 taccttaaaa ttcgaggata atttattctc tacaaatgtt acgccactct gtggcttata
     2461 tttaggtgca gagcaccttg atatttgtag aaaatttatt gaaaatagat attaaggtgc
     2521 agagcaccgt aacaggatgc aaagaatatt gtgttattta ttaaaatgtt tgcattaaga
     2581 ataatttaaa ccaatataat tctttgaatg ataacttgat aattattttt cagcagaccc
     2641 taagtatttg ccaactccct ttttcccgat atcccgaagc ttcgggacgg gacacgtagg
     2701 ggccggggca tgtatcagat caacacaata ttttcttttt tgcattcggc tatcacttct
     2761 tcgggccaga tacttgcctg aatttcgccg atgtgagctt tgcgcaggaa aaacatgcaa
     2821 agtctagact gtccgatacc gcctccgatg gacaatggca gggtgtcgtt catcagtctt
     2881 ttgtggaagt acaagtccag gcggtcttcc ttgccggaaa gcttgagttg tttctgcagt
     2941 gcttttttgt caacgcgtat tcccatcgaa gatacttcga aaggcatttc cagcaccgga
     3001 ttccataata caatgtcacc gttcagaccc ggcagtccgt cttcgcccgg agtagtccag
     3061 tcgtcgtagt cgggagcgcg tccgtcatgt ctttgtccgt cgctcagttc gcccccgata
     3121 ccgatgatga ataccgcttt gtgttttttg gtaatctcat actccctttc tttgggcgta
     3181 agggacgggt agagctgaca cagctcctcg gaatgaatga atgctatttc cggtggcagg
     3241 catggtctta tttcgggaaa gctctcgctt accaggtatt cggtgcggag gaatgctgca
     3301 tatattttat tgacaatgga tttcagaaag tcaatatttc tttcatttgc ttccatcaca
     3361 cgttcccagt cccactggtc aacgtaaagt gaatgcagat tgcccagttc ctcgtcagca
     3421 cggatggcat tcatgtcggt ataaattccg taaccgtttt caatgttgta atcggcgagg
     3481 gtaacgcgtt tccattttgc cagcgaatgt acgacttccg cgcgtgcgtc ttccatgtcc
     3541 ttaatcggga aattcacggc acgttccaca ccgttcaggt catcgttcag tccggtgccg
     3601 cgtaaaacaa aaagaggggc ggtcaccctt cttagccgga gttctgccga aaggttggcc
     3661 tgaaagaaat cttttatttt ctgtatccct aactcagtct gctgaaggtc aagtatggga
     3721 gtataatttt ggggtttgat gaaataactc atgtttgctg atgtaatttt ttaatatgtt
     3781 ttgtctggca aaaatactaa tattatttta taggacaatc aaaatgtatt gaaattgcat
     3841 aaaacaatgt cataattaca ttaaaattgc ttaaacgtgg attgtttgca taaaaaaaca
     3901 gtttctgtgt tcacaaaaac tgttttctgt tgagtttatt tctgcaggtt atttttcttt
     3961 cggcgcgatt ctttcgtatt ggcagaatgt ataggggtag ggattttgtt catctgcgtt
     4021 gaattcttca cgactgattt ctttccatac ttttaaattg attttcggga agaaagtatc
     4081 tgcttcaaaa ttggcgtgta cccaggtgat atacatgttg tctaccattt ttatggcctg
     4141 actaaagatg gaagctccac cgataataaa cacttcgtct tcgtgttcac acaagtcaag
     4201 ggcatccttg atggaggtgg cttcgtaata cttatcaaaa tcgccttctg gaatggaggt
     4261 gagtacgata tttttcctgt ttggaagggg gccattgggc aaactctcaa aagtgcgttt
     4321 tcccatcaca accgcatgac ctgtagtcag ttgtttgaaa tgtttcaaat cagcgggcaa
     4381 atgccatggt aatttgttgt tcttgccaat tgctaaattg tcagctatgg cagcaattat
     4441 cgaaaccttt gacatgttta tatgtaatta ttttgcttat ttgtttgctg tatcagcgta
     4501 aaagcttgcc aaaattataa aaaagaaatg aataaaccat tcggataatt catttcattt
     4561 cttactcatt tgtgtgttaa aatttcatca aacagcaaca atacccttaa tgtgaggatg
     4621 tgggtcataa ttaaccagtt caaaatcgga gtattggaag ctgaaaatat ctcttacatt
     4681 taaattaatt aacatttgag gcagcggtct gggctcacgg gtaagttgta gttttacttg
     4741 ctcgaggtga ttgagataaa tgtgcgcgtc gcccagggta tgcacaaaat cgcctgcttt
     4801 gaggccggtc acctgagcca tcatctgaag cagcagggcg taggatgcga tattgaacgg
     4861 aacacccaga aaaatgtcgg cgctgcgctg gtaaagttgc agactcaacc gtccgtcagc
     4921 tacgtaaaac tgaaagaaag catggcaggg ggggagattc atgttaggca gatcggctac
     4981 attccaggcg ctcacgatga tgcggcggga gtccggattg tgtttgatcg tttcaaccgc
     5041 ttcggtgatc tgatcgatgg aaccgccctt gtaatcgggc cacgaacgcc actgatagcc
     5101 gtaaatatgt ccgaggtcgc cgttttcgtc agcccattca ttccatattc ttactccgtt
     5161 gtcctgaaga aatttagcgt tggtatcacc gttgagaaac cacagaagtt cataaatgat
     5221 ggatttcagg tgaagtttct tggtggtcag gcagggaaat ccttcttcga gattgaagcg
     5281 catctgatgc ccgaatatgc tgatggttcc ggttccggtg cggtcttcct tttttacacc
     5341 ttcggcaaga acccggtcaa gtaagtctaa gtattgtttc atgctgaatt ttttctttgt
     5401 ccgggctgtt aatacagatt gaacgtagtt ttcaaaaccc tgaattcggt ccggcggtta
     5461 atctgattag ctatttcctg ttgaaccgga gttaacgtta ggataaatgc ttcagtcagt
     5521 accgtgtttt ctttcaggaa attatatttt ttcgctgtgc tgatgtcaac ggtaaatggc
     5581 tgttcttctc cgtaacccac agctgtcagt cggttttttg caatgccggc tttaatcaga
     5641 tagtcaacca ccgattgtgc ccggcgttct gatagtatcc ggttggattc gttatttccc
     5701 acgtagtcgg tatgggcgct catctcaatc gtgatattcg gattgtcgtt cagcagttta
     5761 accagctgct gcaatcctgt ttccgattga ggggtaagat cccatttggc aaactcatag
     5821 aatatgttgt tcatctgaac gggtttatac aggggtgtca gttgaaaatc aaccttgaaa
     5881 ctctcgctct ggcggtcgct gacatttttg gtggatattt cattcttttg attaagaaaa
     5941 ccgcgtgcgg aagcaagcat gacgtaatcc acacctttgt tcaatttgta actgtaagtt
     6001 ccgtcgcctt ttgcctggat gcgtgcattt tcgccattat ttcctataat acggattttg
     6061 gcatcgggca cggtttcgcc tacaggatcg gtgatttttc cctgaagcac atattccagc
     6121 gcgggtaatt caaaactcca gagagcgtcg tagcctctgc tctcgtttcg gtttgacgag
     6181 aaataaccac tttctgtttt tccggcaaat gccatcccga aatcatcagc ggaggaatta
     6241 attggaacac ccatgttttt cacttcccag cctccctctt ttaatggttt ggcttcaaat
     6301 atatcaagtc cccccatgcc tggttttcct ttggatgaat agtacagtgt tccgtcttcg
     6361 cgtacggtcg gaaacatttc gtcgtcgggc gtattgatgt cttttcccag attttcgatg
     6421 tatttccatt cgtttccctc gaaagttgct ctccagatat ccttacctcc aaatccgtct
     6481 ttcgagtcgg atacgaaata cagcgtagtg ccgtcgggcg atattgccgg atgtgccact
     6541 gaaatggtgc tgtctctgaa caactggatt ttctgcggtg cgctccaggt tcctccggcg
     6601 cggttcgaaa ggacgattat agttccgcct cccttgtcat cggcaagcag cgaacgggtg
     6661 aaatacatcg aacgcccgtc ggggctgacg gaggcaatac cttcatcatt cagcgaggtt
     6721 acatcacctt ccagctgttc ggtagcttcc cactttccct tgttgttttt ccttgctatg
     6781 aacaggtgat tgtttggctg cccgcttacg ccgttgtttt tacgcactac gttcttctta
     6841 ccgaacttcc ggttggaagt gaaaatcagg acatcggcat cggaacttga aaacaccggg
     6901 ctgaacgagg acgaacggct cacattgaac tctttggatt ttttcaccag gtagcgggtc
     6961 ggactggctt tcaggtcggc agccagcgaa gtcgctttca gaccattctg agccagttcg
     7021 tcatcgggat gtttttccag atacgtagtg taacttttcg cggcttcggc atatttgccg
     7081 tttcgctgct gcatctgtgc cagccgaagg taaaccgtat cattcgtcga tttgtttcgt
     7141 acggcattga gatacacctg ttcggccctg taatgactga tcaggcggta acattctccc
     7201 tgtttgtaag caatctcggc ccgcagcggt ttgtttttcg aagggatgcg ggaatatata
     7261 cttttataca aatcaccggc aacggcgtat tcacccaggg cgtagcgttt gtcggctttg
     7321 gcaagccgcg ctttaatacc gcacgactgc agcgtaatga cagccagcag aatcaacaga
     7381 ccggatgatt tgaattttag cattattatc gggatacttt gtgacttata atctcttatg
     7441 ataaactttt ttgtgacggt tttattgtcc ggcaaccgtg tttttaaacc aattcatgaa
     7501 taacaagtcc cgaacggagc ttgggttcga accaggttgt ttttggaggc atgatgttgc
     7561 cggtatcggc aatgtcgata agttgcttca tcgaaacagg atacaatgcc agcgcgactt
     7621 tcatttcgcc gctgtccacg cgccgtttca actcacccag tccgcgtatg cctccgataa
     7681 agtcaatgcg tttgtcactg cgaaggtctt tgatacccag tatctcatcc aggataaggt
     7741 tcgatgaaat ggtaacgtcc agaacgccaa tcggatcatt gtcgttgtaa gttccgggtt
     7801 tagcagtaag cgaaaaccag ttgttgctca ggtagagcgc gaaattatgc aggctatcgg
     7861 gtttgtagat ttcagctcct ttttcttcca cgatgaagtt ttctttcagt ttttcaataa
     7921 attcttcatc cgataatccg ttcaggtcct taaccacccg gttgtagtca atgatgttca
     7981 gctgattgtc ggggaagcaa accgccatga agtagttgta ttcttcatca ccgcggtgtt
     8041 ttggattctg aagtttcttt tcattgccaa ccagcgcggc agcggcgctg cggtgatgtc
     8101 cgtcggcaat gtacatcgaa ggtatttcag caaagatacg ggttatttgt tcaatgatac
     8161 cgctatcttc aattacccag aactgatgtc tgaatccatc cagttcggca gtgaaatcat
     8221 attcaggttc atttaccgta attccggcaa caatggcgtc caggtcgtcg cggtgaggat
     8281 aggcgaaaaa cacgggttcc acgttggcat tgtttacacg cacatgtttc atgcggtcct
     8341 cttccttgtc gcggcgggta agctcgtgtt tttttatctt accggtcatg taatcgtcca
     8401 cactggcaca aaccaccagt ccgtactgag tgcgtccgtt catggtctgg g
//
LOCUS       DAOPYB010000136         8381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_10141_length_8381_cov_9.014653, whole genome shotgun sequence.
ACCESSION   DAOPYB010000136 DAOPYB010000000
VERSION     DAOPYB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10141_length_8381_cov_9.014653"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PKH58_09860"
     CDS             complement(<1..827)
                     /locus_tag="PKH58_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89378.1"
                     /translation="MKKFTILLLITLCSISAFSTRYLVQPGSAGAATWRTAGAGETLV
                     DLTAEGKTLNAWLTATFTSFPEGDQIWLIKGIYQVNAIFNIPANFTIYGGFAGNETTI
                     DERIKGSNPWDFSNETTLDGNNATAIWGCASNRTALIDGITFTKGYISGNSAGLTTRL
                     GVVVRNSKFLENNATGQGGAILMNAGGEVYDSYFYNNSANLGGAVHIGGVGASVVSGC
                     LFENNKAITGANKQGGAIRSQSTVATIINSKFINNDAAGNGSAIYTQVDTDKANKII"
     gene            complement(974..2494)
                     /locus_tag="PKH58_09865"
     CDS             complement(974..2494)
                     /locus_tag="PKH58_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNX89379.1"
                     /translation="MNNTQKIFTGLLSITFAGGICGQTYNRPNIVYVFPDQFRNNAMN
                     FWNEDAFSKHVNFKADPVHTPNLDKFAKESVVFSRAQSNCPLSSPHRGMLLTGMYPDG
                     NGVSLNCNSTRPCSNLNQDAVTISDVLSQNGYDCAYIGKLHVDYPTKNNPQNPGTYVE
                     DKNPVWDAYTPPERRHGFNFWYSYGTFDEHKTPHYWDTNGERHEIREWSPKHEADKAI
                     DYLQNVRDKSKPFFLMVSMNPPHSPYESLNDVEIEDYNLYKDTPLNNLLVRPNVNPEL
                     KKKQACAPFYFAAITGVDREFGRILSALKEMDLDKNTIVVFSSDHGETMASHVEDPKN
                     SPYTEAMNVPFIVRYPEKLKPQISPLLLSTPDIMPTLLSLAGLNKQIPASVQGHDLSA
                     LIQKYNKSKSPKAVLYIQNVDGKKNPDGKVLNYFPFSRGIKTERYTLSITIDRKKNIK
                     GINFFDDKKDPYQLNNLSTDTHRKEFQKLCRQLSVLLKDSNDPWYREKILNEIIPY"
     gene            complement(2540..4468)
                     /locus_tag="PKH58_09870"
     CDS             complement(2540..4468)
                     /locus_tag="PKH58_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNX89380.1"
                     /translation="MKRIYTPLLILLTVQTYMFPFNRDLLAKSATVDNLTKILIPDRK
                     WVPYPAYSDRGGWDQLTAENKTVLIKRGEKQLGYEWKVIKATDYLEYERSGDRNVMQN
                     PNSANINALADLVLAELAEGKGRFLNQIINGVFYQCERTSWVLSAHLPLQLTGRTLPE
                     HTDVVIDLGSGDFGSFLAWTYYFLHTEFDKVNPVISKRLHDELYNKIILPYRKEDRYW
                     WMGFKPRQDGLVNNWNPWINCNVLQCIALLEDDQTQYAKDVYKTMLSVDKFINYVKED
                     GACEEGPSYWAHAAGKLYDYLEVLSWITNNKITLFENRMIKDMGEYISKSYVGNDWVV
                     NFADASARFSSDAGLIFRYGKAINSSQMQQFASYLVHKQGKKALADDSDLSDMFRKLE
                     SLKYYQELMNAPSMLSTSEVTAYPKTQFYYLRNKNNLFAAIKGGYNAESHNHNDAGTF
                     SLWLDGMPVFIDAGVGTYTRQTFGAERYSIWTMRSGYHNLPDINGQEQRFGKDFKAGN
                     IVFDAVKKSVSLDIAKAYSDSAAVQSWTRKYQLNDNELEITDKYNLKAAIYPNEIHFL
                     VWGKLVKSDGKLLVDAGNRKMELQYDKNQFTSRTDTIALSDVRLSKVWGNEIYRFTLT
                     SKSKNKTGTYKYIIRKMK"
     gene            complement(4614..>8381)
                     /locus_tag="PKH58_09875"
     CDS             complement(4614..>8381)
                     /locus_tag="PKH58_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX89381.1"
                     /translation="VYIQTQNGVDEYNLQTSTISAIENKQVDAISYGSYNLWIADGVN
                     LFSYKDGEKKLYAQLKQSKSAIKVIYQTQDNRIFLGTLSSGVFIINENKQVKRIIPDC
                     SQVSSIFEDSKKNIWIGTWHKGLYKLLRNGQIIVYTKSADKNTGISSEFVRDVCEDND
                     GNIWIGTNNGLDKFTTATGTFKHYGIDENNNMQLSNESVWSLMKDKHGTIWVGTYFGG
                     VNYFNPDMNFYTYHNLQKGIFQNKPFPIISTIVEADNENLFLCTEGNGLIYYNLKDKT
                     YRNFTSQQNNPNSLSSNNIKTACYDATKKVLWLGLHLGGINKLDLNTFKITRLNEIKP
                     GFAQSDIVRTILPYQDKYLIATYNGLYLFDKNTEQFTLFSEKLHKKVSYFVDIKLDKK
                     DNLWIASRGLFRYNIKTGKLQSYMPKPADNTSLSNLNVVKIIIDSKDRVWIATVGGGI
                     NLYNEKTDNFTRYDKATSNLKNDYISNIIESPNGNIIATTTQGFSILNTKNNNIYNYG
                     SENGLPLNSIYNGGVCVLKSGEIYMAGMNGMVSFYEDKLTLPQRFFNIRLSDLWINNK
                     LIVPDDETSILKKNLPYTNSIKLNYKQSTVTIEFASNNYISANQPLYRYKLQGLSGDW
                     VNLPLGSNKLNFMNLTPGKYKLILEAVSQLDGSVIDSTNLEIKMSPPFYQSWIAYLLY
                     IIVIVFAVSRYLSFTRSKLLLKTSLEYEKKEKQQLEEVNQSKLRFFTNISHEFRTPLT
                     LISGQVDMLLQTHNIAPSVYNKILNIKRNTHNMQNLINELLEFRKSEQGFLTIKVHEQ
                     NFIIFLKEIYLSFHDYAKYRNIKFNFNCTEEEIMLWFDPNQIQKVFYNLISNAFKYTK
                     ENGAIDISVKQTDENVIVDIVDSGIGIEAEALGKIFDRFYQAENGLSINNLSQGTGIG
                     LALTKNILELHAASIDVHSEKNKGTDFSVILKKGSSHYTDDQKTDIGNIDIQRVDYTD
                     IPDEEFMQDIINSQGKADKNAYSMLIVEDNAELREMLAKIFDPIYKIYTAVDGEDGLK
                     KAIEYQPNIILSDLMMPNMSGSEMCARIKNNFSVCHIPVVLLTAQTAMEYNIEGLRLG
                     ADDYITKPFNVKVLITRCNNLVNGRKILQERFSKQTDISPAIIATNELDKVFIEKAFS
                     VIEKHLDNPNFDIILFSAEMALGRTKLFSKIKGITGQTPNDFILNYKMKKAGDLLINH
                     PEYNIADITYTLGFNTPKYFAKCFKEQFGVSPSGYRKKHTGVDSEDDNEPI"
BASE COUNT         2241 a         1619 c         1626 g         2895 t
ORIGIN      
        1 ttgataattt tattggcttt gtctgtgtca acctgagtgt aaatagcact gccattacca
       61 gctgcatcat tgtttataaa tttgctattg ataattgtgg ctacagtact ctgacttctt
      121 attgcaccac cttgtttgtt agctcccgtt atggctttgt tgttttcaaa taaacaacct
      181 gaaacaactg acgctcctac tcctccgata tgaacagcac cacctaaatt tgctgaatta
      241 ttgtagaaat aagaatcata aacttcacct cctgcattca taagtatagc tccaccttgc
      301 cccgtagcat tgttttccaa aaacttacta tttctgacaa ctactcctaa acgcgtcgtt
      361 aatccggcac tgttccctga tatatatcct tttgtaaaag tgattccgtc aattaatgct
      421 gttctgttac tggcacaacc ccagattgca gttgcattat taccatcaag tgttgtttca
      481 ttgctaaaat cccaaggatt actaccttta atacgttcat caattgttgt ttcgtttccc
      541 gcaaatcctc cataaatagt gaaatttgca ggaatattga aaatggcgtt cacctgataa
      601 atacctttta ttaaccatat ctgatctcct tcagggaaag aagtgaatgt tgcagttaac
      661 cacgcattta atgttttgcc ttcggctgtt aagtcaacta aagtttcacc cgctcccgct
      721 gttcgccatg tggctgctcc ggcagatccg ggttgtacaa gataacgggt agaaaatgca
      781 gagatactgc atagggtgat aagtagtaaa atagtaaatt ttttcatgat gtccaatttt
      841 aatttgaatg gattaattgt aatttctgcc acaaaagtat gtggttatca gaaaggcaat
      901 ggcccaaaaa agttcaaaaa agcacctttt ttagttcgca tctacataaa attggcatgg
      961 aaaagtttgc atatcagtat ggaataattt cgttcaatat tttctcccga taccagggat
     1021 cgttggaatc ctttaataaa acactgagct gcctacatag tttttgaaat tctttccgat
     1081 gggtgtctgt cgataaattg tttagttggt atgggtcttt tttgtcatca aagaaattaa
     1141 ttcccttaat atttttcttt ctgtcgatag ttatagacaa ggtatatcgt tcagttttta
     1201 tgccacggct gaagggaaaa taatttaaaa cttttccatc aggatttttc ttgccgtcaa
     1261 cattttgtat gtataaaact gcttttgggg attttgattt gttgtatttt tgtattaatg
     1321 ctgataaatc gtgtccctgt actgaagcag gaatttgttt attaagtcct gccaatgaca
     1381 gcagggtggg cataatgtca ggagttgaca gcagcaaagg ggaaatttga ggtttcagtt
     1441 tttcaggata gcgtacgata aacggaacgt tcattgcttc tgtataaggt gaatttttag
     1501 ggtcttctac gtgacttgcc atagtttcac catgatcgga actaaaaaca acaatggtgt
     1561 ttttgtcaag atccatctct ttcagagcgg ataatatccg gccaaattcg cggtcgacac
     1621 ccgttatcgc tgcaaaataa aatggagcgc atgcttgttt tttcttcaac tcaggattta
     1681 cattcggacg taccaataaa ttgttcagag gtgtatcctt gtacaaattg tagtcttcaa
     1741 tttccacatc gtttagtgat tcataaggac tatgtggcgg attcatgctc accatcagga
     1801 aaaacggttt gcttttatcc ctgacattct gcaagtaatc aatagctttg tctgcttcgt
     1861 gttttggcga ccactcccgg atttcatgac gttcgccgtt tgtgtcccag taatgtggtg
     1921 ttttatgttc atcgaatgtt ccgtatgaat accaaaaatt aaatccatgc cgtctttcag
     1981 gcggagtata tgcatcccac actggatttt tatcttctac gtacgttccg ggattttgag
     2041 gattgttttt ggttggatag tcaacgtgta atttgcctat gtaggcacag tcataaccat
     2101 tttgcgatag tacatcactg attgtaacgg catcctgatt taaattgctg catggacggg
     2161 ttgagttaca gttcaatgat actccgttgc catcgggata catacccgtg agtagcattc
     2221 cacggtgagg gctgctcaga gggcaattgc tctgagcacg tgagaatacg acagactctt
     2281 tggcaaattt atccagatta ggagtgtgaa caggatctgc tttaaaattt acgtgcttgc
     2341 tgaatgcatc ttcattccag aagttcatag cattgttgcg aaactgatcg ggaaagacat
     2401 aaacgatgtt tggacggtta taggtttgtc cgcaaatacc tcctgcaaat gttattgaca
     2461 gtaatccggt aaatattttt tgtgtgttat tcattttttt tctgtattta ttatggtgtt
     2521 gaaaagccgg tgaagtaaat tatttcattt ttctgatgat gtatttgtat gtcccggttt
     2581 tgttttttga cttcgaagtt aatgtgaaac ggtaaatttc attaccccat acctttgaaa
     2641 gccgaacatc gcttaaagct atagtgtctg tgcgggatgt aaactgattt ttgtcatact
     2701 gtagttccat ttttctgttg ccggcatcaa ccaacaactt tccatctgat ttcactaatt
     2761 ttccccatac caggaaatga atttcattcg ggtatatagc agctttaagg ttatatttat
     2821 cagttatctc cagctcatta tcatttagtt gatattttct tgtccaactc tgaactgcag
     2881 ctgaatcgga atatgctttt gctatatcaa gagaaactga ttttttaacg gcatcgaaca
     2941 caatattacc ggctttgaag tccttcccga atctttgttc ctgcccgtta atatccggca
     3001 gattatgata accgctgcgc attgtccaga tactatatcg ttcggctccg aaagtctgac
     3061 gtgtataggt ccctacaccg gcatcgataa aaacaggcat gccatccagc cacaatgaaa
     3121 atgttccggc atcgttgtga ttatggcttt ctgcattata acctcctttg atggctgcaa
     3181 acaggttgtt tttgtttctc agataataaa attgtgtttt tggataagcg gtcacttccg
     3241 aagtagaaag catcgaaggc gcgttcatta actcctgata gtattttaat gattccagtt
     3301 ttctgaacat atcgctcaga tcgctgtcgt cagcaagggc tttcttacct tgtttgtgca
     3361 caagataaga tgcaaactgt tgcatttgac tactgttaat tgctttccca tacctgaaga
     3421 tcaaccctgc atctgatgaa aatctggcgg atgcatctgc gaagttgaca acccaatcgt
     3481 ttcccacata acttttcgag atgtattcac ccatatcttt tatcattcta ttttcgaaca
     3541 gcgtgatttt attattggta atccacgaca atacttctaa ataatcataa agttttccgg
     3601 cagcatgtgc ccagtaggaa ggaccttctt cacaggctcc gtcttctttt acatagttta
     3661 tgaatttatc cactgaaagc attgtcttat atacatcttt ggcgtattgt gtttggtcgt
     3721 cctcaagcaa tgcaatgcat tgcaatacat tgcagtttat ccagggattc cagttgttca
     3781 ctaatccatc ctgtcgcggt ttgaaaccca tccaccagta acggtcttct tttcggtagg
     3841 gcagaataat tttattatag agttcatcgt gcagtctttt tgaaatcacg ggattaacct
     3901 tgtcaaattc ggtatgcaaa aaatagtatg tccaggcaag gaatgaaccg aaatcaccgg
     3961 aacccagatc aattacaacg tcggtatgct cgggaagtgt ccgccctgtt agctgcagtg
     4021 gaagatgagc cgaaagtacc cacgaagtac gttcacactg gtaaaaaaca ccgttaatta
     4081 tttgattcag aaagcgccct tttccttcag cgagttctgc cagtaccaga tctgcaagtg
     4141 catttatgtt agcagaattt ggattttgca ttacattcct gtcaccgctt cgttcatact
     4201 ccagatagtc tgtggccttg attactttcc attcataacc aagttgtttt tcgccgcgtt
     4261 ttatcagcac ggttttattt tcggctgtaa gctgatccca tccgccacgg tctgagtacg
     4321 caggataagg aacccattta cggtcaggta tcagaatttt cgttagattg tcgacagtag
     4381 cactctttgc aagtaaatcc ctgttgaaag gaaacatata ggtttgaact gtcaaaagga
     4441 tgagtagggg agtataaata cgtttcatat ctgtaatttt atttttgtgt aaaattacgg
     4501 taaaataatg tccatgaagt actataaaaa gacacaaaaa gggataaaaa agttcaggaa
     4561 gaaagattaa ttaaaaaata cagcgttttt ttacgatcgt tgttatttga gatttatatc
     4621 ggttcgttat catcttcgct gtcaactccc gtgtgttttt tacgatagcc ggaaggtgag
     4681 acgccaaact gttccttgaa acatttggca aaatattttg gagtgttgaa acccaacgta
     4741 taggtgatat cggcaatgtt atattccggg tggttaataa gcaaatcacc tgcttttttc
     4801 attttgtaat tcaggatgaa gtcatttggt gtctgacctg taatgccttt tattttgctg
     4861 aacaatttag tccggcccaa tgccatttct gccgaaaaga gaattatatc aaaattgggg
     4921 ttatcaaggt gtttttctat taccgaaaat gctttttcaa taaatacctt gtccaattcg
     4981 ttcgtggcta taatggccgg tgaaatatcg gtttgtttac tgaatctttc ctgaagtatt
     5041 tttcgtccat tcaccagatt gttacaacga gtaattaata ctttaacgtt gaatggtttt
     5101 gtaatgtaat catccgctcc caaccgcaag ccttctatgt tatattccat ggctgtctgg
     5161 gctgtcagca gcacaacggg aatatgacaa actgagaaat tgtttttaat tcgagcacac
     5221 atttcactgc ctgacatgtt gggcatcatc aggtcactta aaataatgtt gggctgatac
     5281 tcgatggctt tttttaatcc gtcttcacca tcaactgccg tgtaaatttt ataaatcgga
     5341 tcaaatatct ttgctagcat ctcacgcagt tctgcattat cttcgactat aagcatggag
     5401 tacgcgtttt tgtcggcttt tccttgacta ttgattatat cctgcataaa ttcttcatcg
     5461 ggaatgtcag tgtagtcaac ccgttgtatg tcaatatttc cgatgtctgt tttttgatca
     5521 tcagtgtagt gcgatgaacc ttttttcagt attacagaga aatcagttcc tttatttttc
     5581 tcgctgtgaa catcaataga tgccgcatgc agttcaagaa tattctttgt tagtgccaac
     5641 ccaattcccg taccttgtga tagattgttg atgctaagtc cattctcagc ctgataaaaa
     5701 cggtcgaata tttttcccag ggcttcggct tcgatgccaa taccgctgtc aacaatatca
     5761 acaattacat tttcatctgt ctgtttaacc gaaatatcta tggctccgtt ttcttttgtg
     5821 tatttgaatg cgttggatat gaggttgtaa aatacctttt gtatctgatt cggatcaaac
     5881 cagagcatta tttcctcttc cgtgcaatta aaattaaatt tgatatttct gtatttagcg
     5941 tagtcgtgaa atgaaaggta gatttctttg agaaaaataa taaaattctg ttcgtgtacc
     6001 ttaatggtca aaaatccctg ctcacttttc ctgaactcca gcaattcgtt gatcagattt
     6061 tgcatgttat gtgtatttct cttgatgttc agtatcttat tataaactga cggagcgatg
     6121 ttgtgagtct gcaatagcat atccacctga ccactgatta atgtcagtgg cgttctgaat
     6181 tcatgcgaaa tatttgtaaa gaacctgagc tttgattgat taacttcttc aagttgctgt
     6241 ttttcttttt tctcatattc aagggatgtt ttcaacagaa gtttcgaacg ggtgaatgaa
     6301 agatatcttg aaaccgcaaa tactatcacg atgatgtaga gcaggtaagc tatccatgac
     6361 tgataaaaag gaggtgacat tttgatttcc agattggtgc tgtctataac cgatccgtcg
     6421 agctgtgata ctgcttctaa aatcaacttg tatttacccg gtgtgaggtt catgaaattt
     6481 aacttgttcg aacccaacgg aagatttacc caatctcctg aaagcccttg taatttatat
     6541 ctgtacaacg gctgattggc tgaaatgtaa ttgtttgatg cgaactctat ggtaactgtt
     6601 gattgtttgt agttcagttt gatgctgttg gtgtagggga ggtttttttt caaaatcgaa
     6661 gtttcgtcgt caggcacaat aagtttgttg tttatccata aatcactcag cctgatatta
     6721 aaaaatcgtt gaggtaatgt aagtttatcc tcatagaatg aaaccatgcc gttcatcccg
     6781 gccatatata tttcaccgct tttcaaaaca catactccgc cgttataaat ggagtttaac
     6841 ggcaatccgt tttcacttcc gtagttgtag atgttgttgt tttttgtgtt cagaatggaa
     6901 aaaccctgtg tggtggtggc aataatattt ccattgggcg attcaataat gttactgata
     6961 taatcatttt tcaggtttga tgtcgctttg tcgtatcgtg tgaagttatc tgttttttcg
     7021 ttatagaggt tgatgccgcc tccgactgta gctatccaca ctcgatcctt cgaatcgata
     7081 atgatcttca ctacgttcag attgcttaaa ctcgtattgt cagccggttt tggcatatag
     7141 ctttgtaact ttccggtttt gatattgtaa cgaaataaac ctctgctggc tatccataga
     7201 ttgtctttct tgtccagttt tatatcaaca aagtagctga cctttttgtg aagtttttcc
     7261 gaaaagagtg taaattgttc cgtgttttta tcgaaaaggt aaagaccgtt gtaggtggca
     7321 attaagtact tgtcctggta gggtagtatt gttcggacga tatccgattg tgcaaatccg
     7381 ggtttaattt cattcagccg ggttattttg aatgtattca ggtccagttt gttgattcct
     7441 ccaagatgta atccaagcca aagcaccttt tttgtcgcat cgtaacaggc tgttttaata
     7501 ttgttgctgc ttagcgaatt cggattattc tgctgactgg taaaattacg gtatgtttta
     7561 tctttcaggt tataataaat caacccgttg ccttcggtac acagaaacaa attttcgttg
     7621 tccgcttcta caattgtgct gattatcgga aaaggtttgt tctggaaaat gcctttctgg
     7681 agattatggt aggtgtaaaa attcatatca gggttgaaat aatttacacc tccaaaatat
     7741 gttccaaccc aaatcgtacc gtgcttatcc ttcatgagcg accatacaga ctcattggaa
     7801 agctgcatgt tgttgttttc atcaataccg tagtgcttga atgttcctgt tgcagttgta
     7861 aacttatcca gtccattgtt ggtgcctatc cagatatttc catcattatc ttcgcacaca
     7921 tccctgacaa attctgagga tatacccgtg tttttgtctg cacttttggt gtatacaatg
     7981 atttgtccgt tgcgcagtaa tttatataag cccttatgcc aggtacctat ccagatgttt
     8041 tttttgctgt cttcaaaaat cgaagaaacc tgactgcagt cgggtataat tcgttttacc
     8101 tgcttgttct cgttgattat aaaaactccc gaagaaagtg tgcccagaaa aatgcggttg
     8161 tcctgtgtct gataaataac cttaatggcc gatttgcttt gctttaattg tgcatacagc
     8221 ttcttttcac catccttgta tgagaaaaga tttactccgt cagctatcca caggttgtag
     8281 ctgccgtaag aaattgcatc gacctgtttg ttctctatgg ccgaaatagt actcgtttgc
     8341 agattatatt catcaacgcc gttttgggtt tgaatataaa c
//
LOCUS       DAOPYB010000137         8368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_10161_length_8368_cov_10.544809, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000137 DAOPYB010000000
VERSION     DAOPYB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10161_length_8368_cov_10.544809"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PKH58_09880"
     CDS             complement(<1..1038)
                     /locus_tag="PKH58_09880"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNX89382.1"
                     /translation="MATGSTDIWVYADWAGLNGPKLVGVLTAQQAKGRKAFSFEYDKQ
                     WLLRPERILLDPDIQWYSGRQYPVEKENFGLIMDSMPDAWGKALMRRKAAYNAMMGGK
                     PAPALYEIDYLLGVFDESRMGALRFKTEPGGHFLDYKPEAPIPLWIKVKELQHSAMQL
                     ELSDNPDTTHQWLEILIAPGSSLGGARPKANILDETGHPWIAKFPSKNDTTDKGAWEY
                     VAYQLAVRSRIWMAECKIENVTGPYRTFFTKRFDRSGNDRIHFASAMTMTGYTEEMLR
                     ERFASYLEIAEFIQYSCSHVKENLHQLWRRIVFNIAISNTDDHLRNHGFIIRDKGWVL
                     SPAFDLNPSIDK"
     gene            complement(1138..1425)
                     /locus_tag="PKH58_09885"
     CDS             complement(1138..1425)
                     /locus_tag="PKH58_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX89383.1"
                     /translation="MKKQTIFPKQFEILAEMGEQFKLARKRRGLTLVQVAERAGIVRE
                     TLRKIEKGEPGVAMGAYFNVLRVMNLEKDFLKLAADDEFGKKLQDLNLLKK"
     gene            1594..4575
                     /locus_tag="PKH58_09890"
     CDS             1594..4575
                     /locus_tag="PKH58_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNX89384.1"
                     /translation="MEDKINIFKSLFMGREDVFAIRWEKGNKAGYMPAVVYDPYFYRI
                     NKYKKDTENQPPEKNYLPFDDEQILRHLNGHHQVGIYPLLKDNTSWFIAADFDKENWV
                     DECRIFMKFCETYQLPAYLERSRSGNGGHIWIFFKEQYPAVRSRNIILTLLYNSGIIS
                     RYDKNSSFDRLFPNQDFLSGRGFGNLIALPFYKPTFDEGNSCYIDIATLEPYSNQWKY
                     LTEIQRVSTEQLDSLFHSISKVEYETSHEITDKRGLCISLGKELILNKLFIPLMLRDY
                     LKEELNFINTEYIIKKKIGKNTHGIDQYFNFIDETENSLILPKGFTGRLLRYCREYKI
                     NFTFIDSRKKHDSVAFQFNGQFIDFQIPAIEAASKKDFGIIVAPPATGKTIIGLKIIA
                     DRQQPALVVVHRKTLVDQWAERIEAFLGIPKNEIGKIGSGKLKIGNRITIATIQSLSK
                     EVEKSENNPCIEAFGTVIVDECHHIPAETYRKTIGKLNCFYMYGFTATPFRKYSDGKS
                     IFIYLGDIISEINNPEKAFTREAKVIIRETEFDIPFNSKTDQFETLSKMLVQDSARNK
                     QILSDVTRELKSGKKAVIITERREHIDSLYQFLKQSFEVVTLSGEDSEGSRELKWRTL
                     KNGNYQVLITTGQFFGEGTDLQNANCLFLVYPFSFKGKLIQYIGRVQRSTNTPTIYDY
                     RDNRISYLEKLFLKRNTWYRKIGKQATLFDDPDEEIQPINKGIRTFEKNIFIPIETLD
                     FRYGGIAFKFTIPDLQKELEFEIENDALRPEFEVLKPYFSKVLHSKNISAQIHVEFEY
                     GKLVSQLATSTEVDRINREIIEGVRFRYFSKKYLGKNFLPGKADNLLDSKHLQTEGEL
                     YSSGEELVGDILNQKNYKHSRYLRYLANNHESTIFKIRFVLHPFSFVFLLKGEDQLHF
                     IMETLDTEEATYIWHYDKTAHNLPDILKEINNHLSIIRNLGRQAFLEHAPSNFSRIVH
                     DYSDEKKGFINWKDALEETLV"
     gene            complement(4607..4855)
                     /locus_tag="PKH58_09895"
     CDS             complement(4607..4855)
                     /locus_tag="PKH58_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89385.1"
                     /translation="MKYSNAIDMHYEIAEKVFYWLTIKLGGKEIIVLYERCYGYGLGV
                     NSAPGFVFYCPGVARYYFIFSSQFQTTNNLDIEARNKS"
     gene            4881..7205
                     /locus_tag="PKH58_09900"
     CDS             4881..7205
                     /locus_tag="PKH58_09900"
                     /inference="COORDINATES: protein motif:HMM:NF019509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNX89386.1"
                     /translation="MITPFFNKEIRPQLIRLINEAKYEIVASVSWFTDKAIYDALSHA
                     ISRSIKLSLIIQDDIINQTAPFNLEELAQAGARIYLWNTALLGTMHHKFIVVDSAKVM
                     TGSYNWTNAANFQNREDANLIIGEDKLCEKYLFEYRQMSELVENSLLSPGIDLRSNGI
                     NEFGKFDQKFITDNNDSLNENISNFSDYSEIGDFYYGLAKVERNYKYGFIDNNGKEII
                     PLVYDSASDFKNGAAVVMKCDTYYFKDANHEFMLKYSGYGSRNGIKYYQVDSKYALID
                     IHGNELTGFIYDAIYPDLNGFYKVEIGRQFGIISSEGQEIVPPVYHWIYDLEEGFSRV
                     MIDRKYGLFDENWNLIAPAKYDNEDFLFENGLSEVKSDKKVALIDKLGNQIIPFKYAN
                     DHYHFYCGLALVNFNDKFGYINNSGEEVIPVIYEDASHFEEDIAKVKFNGKYGFINSK
                     GEVAIPIIYDNSDIYFHNNLAKVILGQKSGFIDKKGKVAIPLLYDEVWSFNDGLARVR
                     IGKYVGFIDTNGSEVIPVIYENLDIDFPVTLSKVMRNGLWGFIDTKAKEVISLKYDKV
                     SSFSEGLAIVYRDGICKLIDENECIVNSNLPLYDIGELKFSENLARVKLNEKFGYINK
                     TGQQIISPLFDKANHFVGGLAIVSISDKYGYIDSVGNTFIECKWTEDEICRVRVTGFD
                     NFPLGMEDFDTSRLFLIESCLNYKRGYPYWILSDENNNKRYINFRKLCSLADKESDSE
                     ILVNNEYRSALIGELCSINWDMVLGCNQGTLYIA"
     gene            7513..8139
                     /locus_tag="PKH58_09905"
     CDS             7513..8139
                     /locus_tag="PKH58_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89387.1"
                     /translation="MHTSKNGAILFSDIISLQYPHGKWGIEYDDQYSPGGLNMFMPVS
                     SLTDTRINFNLFLGNDGHVGIGTGAPQAKLQVTDGDIFIENIDRGIIMKSPDGKCWRG
                     TLNNQGLLVFTELPDCIITDSKTPEPVNTASVYPNPAGDKLNIKPGKFASHQAEVTVI
                     SATGTIEMKESLSAAQSACIDIQNLKPGNYILQITNGKELESVKFIKQ"
BASE COUNT         2607 a         1552 c         1672 g         2537 t
ORIGIN      
        1 tttatcaatt gaaggattca gatcgaaagc cggagataaa acccatcctt tatcccggat
       61 aataaaacca tggttacgca ggtgatcatc tgtatttgaa atagcaatgt tgaatacaat
      121 tctgcgccat aactgatgca ggttctcttt cacatggctg catgaatact gaatgaactc
      181 agcaatttca aggtagctgg caaacctttc cctcaacatc tcctctgtat atccggtcat
      241 ggtcattgct gaagcaaaat gaatacggtc attacccgat cggtcaaagc gttttgtgaa
      301 aaaggtccgg tatggacctg taacattctc aattttgcat tctgccatcc atatcctgct
      361 cctgacagct agttgataag caacatattc ccaggcgcct ttatctgttg tatcgttttt
      421 cgaagggaat ttagcgatcc atgggtggcc tgtttcgtca agaatattcg cttttggtct
      481 ggcacctccc agcgatgaac caggtgcaat gagtatttca agccattgat gggttgtatc
      541 cggattatcc gaaagttcca attgcatggc gctgtgctgg agctctttta cctttatcca
      601 cagcggaata ggagcttcag gcttataatc caggaagtga ccgccaggct ccgttttaaa
      661 ccggagtgca cccatgcggc tctcatcaaa taccccaagc aggtaatcaa tttcgtacaa
      721 agcaggggca ggctttcctc ccatcatagc attgtaggcg gcttttcttc gcataagggc
      781 tttcccccac gcatccggca tcgaatccat aatcagtccg aagttctctt tttccacagg
      841 atactgcctt cccgaatacc actgaatatc agggtccaga agaatccttt ccggtcgcaa
      901 cagccactgt ttatcgtatt caaagctgaa tgcttttcgc cctttagctt gctgtgccgt
      961 cagcactcca accaatttcg gtccgtttaa cccggcccag tcggcatata cccatatatc
     1021 tgtactgcct gtagccatct ttaatttgat taaatcttat tacatctggt ggattgatgc
     1081 cgatgttatg actgtttcag gacaattgat tggcctgtta cggacatcca aatggtatta
     1141 tttcttcagt aggttaagat cctgaagctt tttaccaaac tcatcatcag cggcaagttt
     1201 caggaaatcc ttttctaagt tcattaccct gagaacattg aaataagcgc ccattgctac
     1261 gccgggttct cccttctcta ttttacgaag agtttcacgg actatgccgg cacgttcggc
     1321 tacctgcaca agtgtaaggc ctcttcgctt acgggcaagt ttgaactgtt cccccatttc
     1381 agccagaatt tcaaactgct tagggaaaat agtctgtttc ttcatattcg ttatgacgat
     1441 tattatgggc aaaaataata ataatggtaa taatattagc cataatgaaa attattgtgc
     1501 ttgtgatcac ttaattaatc actcatcaaa tgggtatttc tgatatattt atagctgcaa
     1561 atccgttttc agctgagaat cagttttaat ttgatggaag acaaaatcaa catatttaaa
     1621 tcattattta tgggtcggga agatgtattc gccatcagat gggagaaagg gaataaagcg
     1681 ggctacatgc ctgctgtggt ttatgatcct tacttttacc ggattaataa gtataagaag
     1741 gatactgaga atcagcctcc ggagaaaaat tatcttcctt tcgatgatga acagatttta
     1801 agacatctta atggtcatca tcaggttggc atctatccgt tattgaagga taatacttca
     1861 tggtttattg ccgccgattt tgacaaagaa aattgggtcg atgaatgtcg gatttttatg
     1921 aaattttgcg aaacatacca attacctgcc tatttggaaa gatcgcgatc aggcaatgga
     1981 gggcatatct ggatattttt caaggaacaa taccctgcgg ttcgcagcag gaacatcatc
     2041 cttactttat tatataattc ggggattatc tcccggtatg ataaaaactc tagcttcgat
     2101 agattattcc caaaccagga tttcctttct ggaagaggtt tcggaaacct gatagcatta
     2161 ccattctata agccaacctt cgacgaaggg aatagttgct atatcgatat cgcaacattg
     2221 gaaccttatt ccaatcaatg gaaatatctt accgaaatcc aaagagtttc aactgagcag
     2281 ctcgatagct tgttccattc aatttcgaaa gtggagtatg aaacttctca tgagattacc
     2341 gacaagagag gtctttgcat cagcctcggc aaggagttga tactgaataa attatttatt
     2401 ccgctaatgc ttcgggatta tctgaaagaa gaactgaact tcatcaatac cgaatacatt
     2461 attaaaaaga aaataggtaa gaatacgcat ggaattgatc agtattttaa tttcattgat
     2521 gagacagaaa attctctcat tcttcctaaa ggatttacag gcaggctatt gcgttattgc
     2581 agggagtaca aaatcaattt tactttcata gattcgcgga aaaaacatga ttccgttgct
     2641 tttcaattca acggtcaatt catcgatttt cagatccctg ctatcgaagc tgcttcaaaa
     2701 aaagattttg gaataattgt ggctccacct gctaccggca aaactattat agggttaaag
     2761 ataattgctg acaggcagca acctgctttg gtcgttgttc atcgtaaaac ccttgtagat
     2821 caatgggctg agagaattga ggctttctta ggcatcccga aaaatgaaat agggaaaatt
     2881 ggatcaggga agttgaaaat tggcaacagg ataacaatag caactattca gagcctttca
     2941 aaagaagtgg agaaatcaga aaataatcca tgtatagaag cattcggaac tgtcattgtt
     3001 gatgaatgcc atcatattcc tgctgaaact taccgaaaaa ccattggtaa actgaattgc
     3061 ttctatatgt atggattcac ggcaactcct ttcagaaaat atagcgatgg caagtcaatc
     3121 tttatttatc tgggagatat catttcggaa attaataatc ccgaaaaggc tttcactcgt
     3181 gaagcaaaag taattatccg cgaaacggag tttgatatac ctttcaattc aaagaccgac
     3241 cagtttgaaa ctctttcaaa gatgctggtt caagattcgg cacgtaacaa gcagatattg
     3301 agtgatgtta caagggaatt aaagtcaggt aagaaggctg ttattatcac tgagcgcaga
     3361 gaacatattg attcattgta tcaattcctg aaacaatcct ttgaggttgt gactttaagc
     3421 ggcgaagatt cagagggtag tcgcgaattg aaatggagaa cacttaaaaa tgggaattat
     3481 caggtactga ttacaacagg gcaatttttt ggtgaaggaa cagacttaca aaatgcaaat
     3541 tgtttgtttc ttgtttaccc gttttcgttt aaagggaaat tgattcaata tataggaagg
     3601 gttcaacgat caacgaacac tccgactata tatgattatc gggataatag gatcagttac
     3661 ctggaaaaac ttttcctgaa gcggaacact tggtacagga aaatcgggaa gcaggcaact
     3721 ctgttcgacg atccggatga agaaatccag ccgataaaca aaggaattcg gacttttgag
     3781 aaaaacatat ttattccaat cgaaactttg gatttccgat atggtgggat cgcctttaaa
     3841 ttcacgatac ccgacttaca gaaagaatta gaatttgaaa ttgaaaatga tgcacttcgc
     3901 cccgaatttg aagttttgaa gccgtatttc tctaaagtgt tgcattccaa gaatatttcc
     3961 gcacaaattc acgtcgaatt tgaatatggt aaattggttt ctcagttagc tacttcaaca
     4021 gaggttgaca ggataaacag ggaaattatc gaaggcgttc gctttcgcta tttttcaaaa
     4081 aaatatctcg gtaagaattt tctgcctggc aaagctgata atctcctgga ttctaaacat
     4141 ctccagactg aaggagaact atatagttct ggagaagaat tggtggggga tatcctgaat
     4201 caaaagaatt ataaacactc aagatatctg aggtatttgg cgaataatca tgaatcaaca
     4261 atttttaaaa ttcgttttgt tcttcatcct ttctcattcg ttttccttct caaaggagaa
     4321 gatcaattac actttataat ggagacactc gacacagaag aagcaactta tatttggcat
     4381 tacgacaaga ccgctcataa tctgccagat atattaaaag agattaataa ccatctgtct
     4441 attatccgta accttggcag gcaggctttt ctggaacatg caccttcaaa ttttagtcgc
     4501 attgtgcatg actattctga tgagaaaaaa gggttcataa attggaaaga cgctttggaa
     4561 gaaacattag tgtaatgcac aatttctttt ttctataatt aaacgtctaa gacttatttc
     4621 ttgcttctat gtccaggttg ttcgttgttt gaaactgcga actaaatata aaatagtaac
     4681 gagcaacccc agggcagtaa aaaacaaacc ccggtgcgct attcactccc aaaccataac
     4741 cataacaacg ctcataaagc acaataatct ccttcccgcc aagtttaatc gtaagccagt
     4801 agaaaacctt ttctgcaatt tcataatgca tatcaattgc attcgaatat ttcattaatt
     4861 tgcctttcaa ggctcaaatc atgattactc cattttttaa taaagaaatt cgacctcaac
     4921 taattaggtt gatcaatgaa gctaaatacg aaatagtcgc ttctgtttca tggtttactg
     4981 ataaggctat ttatgatgca ttatctcatg ccatatcaag atccattaaa ctcagtttga
     5041 tcattcagga tgatataatc aatcaaactg caccattcaa tttagaagaa ttggctcagg
     5101 ccggtgctcg gatttatcta tggaatactg cacttcttgg taccatgcac cataagttta
     5161 ttgtggttga ctcagcaaaa gtcatgacgg gtagttataa ctggacaaat gccgctaatt
     5221 tccagaacag agaagatgca aatctaatta ttggagaaga taaactttgt gaaaaatacc
     5281 tgtttgagta tcgacaaatg agtgaactag tagaaaatag tctcctctct cccggtattg
     5341 atctgcgctc gaacggtatc aacgaatttg gtaagtttga tcagaagttc attacagaca
     5401 ataatgattc attgaatgaa aatatttcaa atttttcaga ttatagcgag atcggtgact
     5461 tctattatgg ccttgcaaaa gttgaaagaa attacaagta tggttttatt gataataatg
     5521 gaaaggaaat aattccattg gtgtatgatt ccgcttctga ttttaaaaat ggagcagctg
     5581 tagtaatgaa atgtgatact tattatttta aagatgctaa ccatgagttc atgctaaaat
     5641 actctggtta tggctctcgg aatggcatca aatattatca agtggatagc aaatacgcat
     5701 taattgatat tcatggaaat gaattaacag gttttattta tgatgcaata taccctgatt
     5761 taaatggctt ctataaagtt gaaattggaa ggcagtttgg aatcatcagt tctgaaggac
     5821 aggagatagt tcctcctgtt tatcattgga tttatgattt agaagaaggt ttttcaaggg
     5881 ttatgataga taggaaatat ggtttgtttg atgagaattg gaatctgatt gctccggcaa
     5941 aatatgataa tgaagatttc ttatttgaaa atggtctttc cgaagtcaaa tctgacaaaa
     6001 aagttgcttt aattgataag ttgggtaatc aaattattcc attcaagtac gcgaatgatc
     6061 attatcattt ttactgtggt ttggctttag ttaattttaa tgacaagttt ggttatataa
     6121 ataattcagg tgaagaagta ataccagtta tttatgaaga cgccagtcat tttgaagaag
     6181 acattgcaaa agtgaaattt aatggcaaat acggatttat taattcaaaa ggggaagtgg
     6241 ctataccgat tatatatgat aattcagata tttattttca taataatctt gctaaagtta
     6301 ttcttggtca aaaatctggt ttcattgaca aaaagggtaa agttgcgata cccttattat
     6361 atgatgaggt ttggagtttt aatgatggct tagcacgagt aaggattgga aagtatgttg
     6421 gtttcattga tacaaatgga agtgaggtaa ttcctgttat ttatgaaaat ctagatatcg
     6481 atttccctgt tacattatct aaagtcatga ggaatggact atggggattt attgacacaa
     6541 aagccaagga agtaatttca ctaaaatatg ataaggtttc tagtttctct gaagggttag
     6601 cgattgttta ccgcgatggt atttgtaagc tgattgatga aaatgaatgc atcgttaatt
     6661 caaatttgcc tttatatgat attggagaac tcaaattttc cgagaatctc gcccgagtga
     6721 aattgaatga gaaatttggt tatattaata aaactggtca acagataatc tcacctttat
     6781 ttgataaggc gaatcatttt gttggtggtc tggcaatcgt ttcaatttcc gataagtatg
     6841 gatatattga ttcggtgggt aacactttta ttgaatgtaa atggacagaa gatgagatat
     6901 gcagggttcg tgttactgga tttgataatt ttccacttgg tatggaagat tttgatacaa
     6961 gtagattgtt cttaattgaa tcatgtttaa attacaaacg tggataccct tactggatct
     7021 taagtgatga aaataataat aaaaggtaca ttaatttcag aaaattatgt tcgcttgcag
     7081 ataaagaatc tgacagcgaa atcttagtga ataacgagta tagatcagca ctaataggtg
     7141 aattatgtag tattaattgg gatatggttc ttggttgcaa tcagggtaca ctatacatag
     7201 cgtagatatc tgattcattc aacagaattc atattcgata tttttgaaga tcatcaatag
     7261 aatttagaca cactccaaga atttattaat aagaatctct gctaaaccag tctattgcag
     7321 catgggagta aggtagaggg cagtcaaaaa gcaaccccaa gacagtcaaa ataaaccccg
     7381 gtgcgctatt cacttccttc gcatatccga aaactacgtt cattaagtca aataaccacc
     7441 agccatcctg cctgccttgc gtaagccaaa gcgcagccgg caggtcgtct tccttccccg
     7501 caattttacg taatgcatac ttccaaaaac ggtgcaattc ttttttctga cattatttca
     7561 cttcagtatc ctcacggcaa gtggggcatc gaatacgatg accaatacag tcccggaggc
     7621 cttaatatgt ttatgccggt ttcgagtctc acggacaccc gtatcaattt taaccttttt
     7681 ctgggcaatg acggccatgt aggcatcggg accggagcgc cgcaggccaa actgcaggtt
     7741 accgacgggg acatctttat tgagaacatc gaccggggga tcattatgaa atcacccgac
     7801 gggaaatgct ggcgggggac gctgaataat cagggactac tggtattcac ggaactgccc
     7861 gattgtatca tcacggatag taaaacccct gaacctgtca acacggcttc ggtttatccg
     7921 aatccggccg gcgacaaatt aaatatcaaa ccggggaagt ttgcttctca ccaggccgaa
     7981 gtgacggtga tttcagcaac aggaaccatt gagatgaaag aaagcctatc ggcagctcaa
     8041 tcagcctgta ttgatatcca aaacctcaaa cccggcaatt acatcctgca gataaccaac
     8101 gggaaggaat tggaatcggt taagtttata aagcagtaac cggatatctg cccgaagaat
     8161 cccctgccgc gcttgttatc accgaaggct ttttactctg cggctttccg gagtatatcc
     8221 ggtttgtttg ccggcagctt gtgacagagg taactgaact catctgctat acgcaacgga
     8281 tgagcatccc cggggacgat ccggcagagt cgtagccatc cggcatgtaa atattagcca
     8341 tatttgcata taactttttt attcacta
//
LOCUS       DAOPYB010000138         8254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_10350_length_8254_cov_8.598854, whole genome shotgun sequence.
ACCESSION   DAOPYB010000138 DAOPYB010000000
VERSION     DAOPYB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10350_length_8254_cov_8.598854"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..3091)
                     /gene="polA"
                     /locus_tag="PKH58_09910"
     CDS             complement(296..3091)
                     /gene="polA"
                     /locus_tag="PKH58_09910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNX89388.1"
                     /translation="MLSNKKLYLIDAMALIYQAFYAYRDRPLINSKGVNTSAVHGFAN
                     VLYKLLKEEKPTHIGVAFDTMAPTLRHDDFIQYKANRQETPEDLVSSIPYIKRLIEGF
                     NIPTLFVDGYEADDVIGTLAKEAEQAGYTVYMVTPDKDFGQLVSENIFIYKPARFGDK
                     ASIMGPKEVCEKFGIQNPLQVIDMLGIWGDASDNIPGIYGIGEVWARKLVKEFGSLEN
                     ILANVDSIKPDGIKQKVIAGRELAIQSKALATIILNVPIAFEENKLVMEHPNEHELKE
                     LLDELEMRTFAKRVFTDISLQKQAAAQAQQNFQPDLFSEAQPETTSQDKSSTRNIYNT
                     AHEYILVDTDEKKNALIAELSKSKEFCFDTETTGLDPNSAEMVGVSFCMKPHKAYYIP
                     VPKEYNEACRLLCDFKPVFENAEILKIGQNMKFDLEMLKYYDIEIKGPLFDTMLAHYL
                     IEPDMRHNMDYLSLSYLNYEPVHIEQLIGKKGKNQLSMAMVPAEEIKEYAAEDADVTL
                     QLKQIFEPLLKESNVTSLFREVEIPLIPVLASMETEGVKLDKNALNEYSGQLRTEIQQ
                     LEKEIFELAGMEFNISSPKQLGDVIYGHLKIVENPKKTATKQFSTAEDVLIKLVNKHP
                     IIQKLLDYRSLTKLKSTYVDALPELINPRTGRIHTSYNQAVAATGRLSSNNPNLQNIP
                     VRTEKGREIRKAFVPRNEDYVLVSSDYSQIELRIIASISDETNMIEDFRQGHDIHAAT
                     ASRVYNVPLADVTKEMRRNAKMVNFGIIYGISAFGLAERLNIPRKEAADIIDQYFIKY
                     PAIKKYMENTIASARQKGYVETLLGRRRYLRDINSANGVVRGYAERNAINAPIQGSAA
                     DIIKIAMINLYRKISDQQLRSRMILQVHDELVFDVYKPELEKMLPLIKDEMQLAFKIK
                     VPLDVEISDGNNWLEAH"
     gene            3254..4618
                     /locus_tag="PKH58_09915"
     CDS             3254..4618
                     /locus_tag="PKH58_09915"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89389.1"
                     /translation="MPIMNEYYVYGLIDPRTNSIFYIGKGKGKRVFQHFKEKEDYHTN
                     SEKLKLIAEIQKEGQQVNHIFIGENLKEETALLLEKLLIYRIGRKIFNEGCLTNIVPG
                     GRWNSEASYFLSNEDLPSIEAIKSQYPDLISVLEKFPKISKEFSGLQDPNNSENKTLY
                     IYEKNGTKLYDWDIDYLIKLFGLSSALEIINTLKDTVMPVYARGRLWSKINHEKLEDI
                     SKIPFQEMDIIDFGFVKQINNVLVKKNNLSLKCLFPDGKIHNEIVIISDSNEITLTSY
                     YLNGNKKHITSCIEGKLNGKCRSWHANGQLKEEMEYILDRRISKTCYFPSGVIQMIEN
                     YNNEGNSVSVKTWYDNEQLCFESNENGLSYSYSLAGKIISKSIRSGDLHNGGHLLAWE
                     YSEDGKVKKEIKSYYLNHLLHGYEKSYYDTGELRREVDYTNGPDNKIIKLYKKNGEET
                     IKKT"
     gene            4590..5498
                     /locus_tag="PKH58_09920"
     CDS             4590..5498
                     /locus_tag="PKH58_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89390.1"
                     /translation="MEKKRLKKLNENQTHRISFAKFLFKEAEKKCRLPNPNDSITILN
                     LHDSVDIYIQLLAEFYNCKPDNKTSLMSTVNSINTKLVELKLEKLELSLFDRLNTARN
                     KLKHETIFLNQIDIFGFISGVKNFYIESTPLYFGGLKFESISLVNLVKSPKVKKCLQY
                     AKKEIENKKYNPALEKINEAFGYIINDITTFKSNQGYRVSVKPSKWFHPISSSYIGNF
                     DKHGIKGFNDFERGLKESFDYLNELIIINNFKVNINDYARFKQYKLYGYMSTTTKFTV
                     VGEYKNKIKENEINFCFDFVLQIALS"
     gene            complement(5537..5920)
                     /gene="tnpA"
                     /locus_tag="PKH58_09925"
     CDS             complement(5537..5920)
                     /gene="tnpA"
                     /locus_tag="PKH58_09925"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNX89391.1"
                     /translation="MSTYTQILYQIVFSTKSRKKILQDPDRDEVLKYMSGIIQNKNCH
                     LYRINGVEDHIHILTHLHPSVALADLVKDIKLASSEYIKHGNLCQGFGGWQAGYGAFT
                     YAYKDIDRLIEYVKNQCKYTQNSDS"
     assembly_gap    5938..5947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6230..6802)
                     /locus_tag="PKH58_09930"
     CDS             complement(6230..6802)
                     /locus_tag="PKH58_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89392.1"
                     /translation="MNNNHNTTPSQPVNVDNLISRLQREDKRNKRIMMVMFIIYLAFS
                     LIYLYLFILNPAPSLSFTYRMAGLCFVIAFFIGTVFFWFRYKNLKKLDYTLPLVQVLG
                     KTVERFRFWGAKWIPVLIVVLLVDIGFTISYYHKFEYWDTTFLIKILSIQVIYWGIML
                     VGGLIGYWRWIKRSKTIWKDAKTLLKELKN"
     gene            complement(6795..7313)
                     /locus_tag="PKH58_09935"
     CDS             complement(6795..7313)
                     /locus_tag="PKH58_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX89393.1"
                     /translation="MNPKEEKFRKIVADQQQRIWNICRHYSRSDEDTQDLQQEVLVNV
                     WRSLDNFRGDSALSTWIYRIAVNTSLNYIMKENRRSQFNLTLDHQNINMLMQDEEHEQ
                     KIETEKKLDELHEIINQLNVIDKLLITLSIEKLTTREISDIIGITEPNVRTKLHRIRE
                     ELRQKIQGGNHE"
     gene            complement(7379..>8254)
                     /locus_tag="PKH58_09940"
     CDS             complement(7379..>8254)
                     /locus_tag="PKH58_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX89394.1"
                     /translation="FNQFYVEKNAKRMLKANPANRLLKHLSHCALVYFCPAARNYFMN
                     RWEAPLPTSPSCNSRCVGCISYQPKDHWITSTQNRITFIPTPEEIAEIAVEHLNTAPN
                     PVVSFGQGCEGEPLLVWETLRDAIKLIRTKTHKGIINLNTNASKPEAIEELCKAGLQS
                     IRVSMNSVQEQHYLKYYNPTNYTFADLMESMRIAKKYKIWISINYFVFPGYTDEAGEV
                     AALKKLISEFDISMIQWRNFNIDPEWYSEIVPLPADTHPIGIKNLVDQIQKEFPKLYH
                     GYFNPGEEIINRYLK"
BASE COUNT         2452 a         1515 c         1651 g         2626 t
ORIGIN      
        1 cagcaatagt aattaaggaa taaatttatc aactacattt tagaatagag ccattgtttt
       61 ttgaaggttt ttgttgaatg atacgaaata acaaataaca aatttcaaag agcaaacaaa
      121 ttccaatccg ccatggcgaa taaaatacaa aatccaaaac gcctgtttca tccttcgcgg
      181 cggacttcct tctaattccg acttctgaat tcttcttaat tctgcattca aacttctgat
      241 ccgccgcggc ggacttaatt ctgaattctg ccttctgcct tttgccttct gaattctagt
      301 gtgcctccag ccagttattc ccatcactga tctccacatc taacgggact tttattttaa
      361 atgcaagttg catttcatct tttataagtg gcaacatctt ttccaattcc ggcttgtaca
      421 catcgaacac caactcatca tggacctgca atatcatccg gctgcgaagt tgttgatctg
      481 aaattttcct gtaaaggttg atcatggcaa ttttgatgat gtcggcggca gagccttgta
      541 tcggtgcgtt gatggcgttg cgctcggcat agccccgcac cactccgttg gcagaattga
      601 tgtcgcgtag gtaacgcctg cgcccgagca gtgtttctac gtaccctttc tggcgcgccg
      661 atgcaatggt gttttccata tattttttta tcgccggata tttgataaaa tattggtcga
      721 tgatatctgc cgcttcttta cgcggaatat tcagcctttc tgctaagcca aaagccgata
      781 tgccgtagat aataccaaaa ttcaccatct tggcgttacg ccgcatttct ttggtaacat
      841 cggccaatgg gacattgtaa accctggaag ccgtagctgc atggatatcg tgcccctgcc
      901 tgaaatcttc tatcatattg gtttcatcac tgatggaagc aataatgcgt aattcaattt
      961 gagaataatc ggaggaaacc agcacgtaat cctcgttacg cgggacaaag gctttacgga
     1021 tctcacggcc tttttctgtc cgcaccggga tattctgcag gttcggattg ttcgaactta
     1081 agcgcccggt ggccgccacc gcctgattgt acgacgtatg gatacgcccg gtgcgtggat
     1141 tgatcagctc gggaagggca tccacgtaag tggatttcag tttggtcagc gagcggtagt
     1201 ccagcagttt ttgaatgatc ggatgtttgt tcaccagttt tatcaacaca tcctcggccg
     1261 ttgaaaactg tttggtggct gtctttttgg gattttcaac gattttaaga tgcccgtaaa
     1321 tcacatcgcc aagctgtttg ggcgatgaaa tattgaattc catcccagcc agttcaaaga
     1381 tctctttttc gagttgttgt atctcggtac gaagctggcc cgagtattca ttcagcgcat
     1441 ttttatcaag cttcacacct tccgtttcca tggaggcaag caccggtatc agcgggattt
     1501 caacttcccg gaataaagaa gtaacgtttg attctttgag tagtggttca aagatctgtt
     1561 ttagttgtaa ggtaacgtcg gcatcttcgg cggcgtattc ttttatttct tcggcaggca
     1621 ccatcgccat ggaaagctgg ttcttacctt ttttaccgat cagctgttcg atatgtaccg
     1681 gctcataatt taaataactc agtgacaaat aatccatatt gtgccgcata tccggttcaa
     1741 taagatagtg cgccagcatg gtatcgaaca gcgggccttt tatttcaata tcataatact
     1801 tcagcatctc caggtcaaac ttcatgtttt gccctatttt caatatttct gcgttttcaa
     1861 ataccggttt aaaatcacat aacaaacggc aggcttcgtt atattccttc ggaaccggga
     1921 tgtagtaagc tttgtgtggt ttcatgcaaa acgaaacccc aaccatttca gcggaatttg
     1981 gatctaaacc tgtagtttcc gtatcaaaac aaaattcttt ggacttagat agttcagcga
     2041 taagtgcatt tttcttttca tcggtatcca ccaaaatata ttcatgggcg gtgttgtaga
     2101 tatttcttgt tgaagattta tcctgtgacg tggtttccgg ctgagcctca ctgaacaggt
     2161 ccggttgaaa gttttgttgt gcttgggctg cagcctgctt ttgtaaagag atatccgtga
     2221 acacgcgttt ggcaaatgtc cgcatttcca actcatctaa gagttctttc aactcatgtt
     2281 cgtttgggtg ttccatgaca agtttgttct cttcaaaagc aatcggtacg ttcaggatga
     2341 tagttgccag cgcttttgac tgtatggcta gttcccgtcc tgcaattact ttttgtttaa
     2401 tgccatcagg tttgatggaa tctacgtttg ccaaaatgtt ttcaagagag ccgaattcct
     2461 tgaccagttt acgggcccat acctcaccga taccgtatat cccgggaata ttatcggaag
     2521 catcacccca gatgccaagc atatcaatta cctgtaacgg gttttgtatg ccgaattttt
     2581 cacaaacctc tttgggcccc atgatggagg ccttatcacc aaagcgggca ggtttataaa
     2641 taaaaatatt ttcgctgacc agttgcccga aatctttgtc gggtgtcacc atgtaaacgg
     2701 tatatcctgc ctgttcggct tccttggcaa gggttccgat gacatcgtcg gcttcatatc
     2761 catctacaaa aagtgtggga atattaaatc cttcgatcag tctttttata taaggaatgg
     2821 aagaaacaag gtcttccggc gtttcctgac ggttggcttt gtattgaata aaatcatcgt
     2881 ggcgcagggt gggagccatg gtgtcaaaag caacaccgat gtgcgtgggt ttttcttctt
     2941 tcaataattt atacaggaca ttggcaaagc catgtactgc ggaggtgttg accccttttg
     3001 aattgatgag cggccggtcg cggtatgcat aaaatgcctg gtaaataagc gccatagcat
     3061 caatcaggta gagcttttta tttgacaaca tagtattaat ttaaaatgta aagatagttt
     3121 tttgctgcta aaagtgcaaa attgaatgcc ctcctcttta aaattatttt tgtattattg
     3181 ttttattgag tttacttttg ttaaagaata atgatttcga ttaaagattt tctttggtaa
     3241 atacaatttt tttatgccga ttatgaatga atattatgta tatggcttga ttgatccaag
     3301 gacaaattcc attttttata ttggaaaagg aaaagggaaa agagtatttc aacactttaa
     3361 ggaaaaagaa gattatcata ctaattctga aaaactaaaa ctaatcgctg aaatacaaaa
     3421 ggaagggcaa caagtcaatc atatttttat tggtgaaaat ttgaaagaag agaccgcctt
     3481 acttcttgaa aaattgttaa tttatcgaat tggtagaaaa atttttaatg aagggtgttt
     3541 gacaaatata gtacctggcg gaagatggaa ttcggaagcc tcatattttc ttagcaatga
     3601 agatctacca agtattgaag caataaaaag tcaatacccg gatttgattt ctgttttaga
     3661 aaaatttcca aaaatttcaa aagagttttc aggattgcaa gatccaaata attctgaaaa
     3721 caaaacacta tatatatatg aaaaaaatgg aacaaaactt tacgattggg atatagatta
     3781 tctaataaaa ttatttggtt taagcagtgc acttgaaata ataaatactt tgaaagacac
     3841 tgtaatgcca gtttatgcca gaggaagact ttggagtaag attaatcatg aaaaactgga
     3901 agatatttca aagattccat ttcaagaaat ggatataata gattttggtt tcgtaaaaca
     3961 aataaataat gtccttgtaa aaaagaataa tctttcatta aaatgtttat tcccagatgg
     4021 taaaatacat aatgagatag ttataatttc agattcaaac gagattactt taactagtta
     4081 ttatttaaat ggtaacaaga aacatatcac cagttgtatt gaaggaaaat taaatggcaa
     4141 atgccgcagt tggcatgcaa acgggcagct gaaagaagaa atggaatata ttcttgacag
     4201 acgaataagt aaaacgtgtt attttccttc tggagttatt cagatgattg aaaattacaa
     4261 taatgaaggc aactctgttt cagtaaaaac atggtatgat aatgaacaat tatgttttga
     4321 aagtaatgaa aatggccttt catattcata ttcacttgca ggcaaaataa tatctaaaag
     4381 tattcgatcg ggtgatttac acaatggagg tcatttgtta gcttgggaat attcagaaga
     4441 tgggaaagtg aagaaggaaa ttaaatcata ttatcttaac catttattac atgggtatga
     4501 aaagtcatac tacgatactg gtgaattaag aagagaggtg gattatacaa atggccctga
     4561 taataaaatt attaaattat acaaaaagaa tggagaagaa acgattaaaa aaacttaatg
     4621 aaaatcaaac tcatagaatt tcctttgcaa aatttctatt taaggaagca gaaaaaaaat
     4681 gccggttgcc aaatccgaat gattcgataa cgatactgaa tttgcatgat agtgttgata
     4741 tttatataca gcttttagct gaattttaca attgcaaacc ggacaataag acatcattga
     4801 tgtctacagt taattcaata aatacaaaat tagtagagtt aaaactggaa aaactggaac
     4861 tctctttatt tgatagattg aacacagcaa gaaataaact taaacatgaa acaatttttc
     4921 ttaatcaaat agacattttt ggatttattt caggtgtaaa aaacttctat atcgagtcaa
     4981 cgccattgta tttcggcggt ttaaaattcg aatcaatatc attagttaat ttagtaaagt
     5041 ctcctaaagt gaaaaaatgt ttacagtatg caaaaaagga aattgaaaat aaaaaatata
     5101 atcctgcttt agaaaaaata aatgaagcat ttggttacat aataaatgat attactactt
     5161 ttaaaagcaa tcaaggttat cgtgtgagtg taaaaccctc aaaatggttt catccaattt
     5221 ccagtagtta catcggtaat tttgataagc atggtattaa aggattcaat gattttgaaa
     5281 gaggattgaa agaatcattt gattatctaa atgaactaat aataattaac aattttaaag
     5341 ttaatattaa tgattacgct aggtttaagc aatacaaact ttatggttat atgtcaacaa
     5401 cgacaaaatt caccgtggta ggtgaatata aaaataaaat taaagaaaac gagataaatt
     5461 tttgttttga ttttgtttta caaattgcat tgagttaaca aaagtttttg cattgataga
     5521 ttaattgtgc taattattaa ctgtctgaat tttgggtgta cttacactgg ttcttcacat
     5581 attctattaa cctgtcaata tccttataag cgtaggtgaa cgccccgtaa cccgcctgcc
     5641 agccgccgaa gccctggcaa aggtttccgt gctttatata ttcggaggag gcgagcttaa
     5701 tatcttttac aaggtctgcc aatgctaccg aaggatgaag atgggtgagg atgtggatat
     5761 ggtcttctac accgttgata cggtataggt gacaattctt attttggatg atgccggaca
     5821 tatacttcaa cacttcgtct ctgtcgggat cttgcaggat tttttttctg cttttggtgc
     5881 tgaagacgat ttgatagagt atttgggtgt aagtactcat gattttttat ttattcannn
     5941 nnnnnnnggc ttcatggatg taaaataact tttctatttc catgattttt tatttattta
     6001 accccgttgg ggttggtggt tagttgctgt cactattccg gactgagtcc ggtatattgg
     6061 taactcttgt tataccggcc ttgcttcgac aagcacagca aaagttgtcc ggtatccgga
     6121 cttacgtcct ggattattca tattaaacca cttcgtggtt tgcttaggta aataaatgaa
     6181 cttataatag agttactcaa atctatgcga aatgcgatca atcagcttct cagttcttca
     6241 gctctttcag cagtgtcttt gcatctttcc agattgtttt ggaacgtttg atccagcgcc
     6301 agtatccgat cagtccgcct actaacatga tgccccagta aataacctgt atcgaaagaa
     6361 tctttatcaa aaaggtggta tcccaatatt caaatttatg gtagtagctg atggtgaatc
     6421 ctatatctac caataaaact acaattaaga cgggtatcca ttttgctccc caaaacctga
     6481 atcgttctac cgtttttccg agtacctgta ccaaaggaag cgtgtaatca agctttttga
     6541 ggtttttgta ccggaaccag aaaaaaaccg ttccgataaa aaatgcaatt acgaaacata
     6601 atccggccat gcggtaagta aaggatagtg atggagccgg attaaggata aacaggtaca
     6661 aataaatcag tgaaaaagcc aggtagataa tgaacatgac catcatgatt cgtttattac
     6721 gcttgtcttc ccgctgcagg cggctgataa ggttgtccac attaacaggc tgcgatggtg
     6781 tggtattgtg attattattc atgattacct ccttgaattt tttgacgcag ttcttcacgg
     6841 atgcgatgaa gtttggtgcg cacattgggt tcagtgattc cgatgatgtc cgagatttcg
     6901 cgtgtggtca atttttcaat agacagtgtg atcagcaatt tatcgatgac attcaactgg
     6961 ttgattattt catgaagctc atcaagtttt ttttcagttt ctatcttttg ttcatgctct
     7021 tcatcctgca tcagcatatt aatattttga tgatccaggg taagattgaa ctgggacctg
     7081 cggttttcct tcatgatgta attcagcgaa gtgttaacag caatgcggta gatccaggtg
     7141 ctgagcgccg aatcaccacg aaagttgtcg aggctgcgcc atacattgac cagcacctcc
     7201 tgttgcagat cctgcgtgtc ttcatcggat cgggagtaat gccggcaaat gttccagatt
     7261 cgctgctgtt ggtccgccac tatttttcga aacttttctt ctttcggatt catcgtggtt
     7321 cagagtttga acattggata gtatgaatgg gtatttgtta caaattttaa aatacggctt
     7381 atttcagata cctgttaatg atttcctcac ccggattaaa atatccgtga taaagtttag
     7441 gaaattcttt ttgtatctga tcaacaaggt tttttattcc gataggatgt gtgtcagcgg
     7501 gtagcggaac aatttccgaa taccattcag ggtcaatatt gaaattccgc cactgtatca
     7561 tactgatatc aaattctgat attagttttt tcagtgcagc aacttcaccg gcttcgtccg
     7621 tgtatccagg aaaaacaaaa taattgatag aaatccagat cttgtatttt tttgcaatcc
     7681 tcatgctttc catcaaatcc gcaaatgtat aattggtggg attatagtat ttcaaataat
     7741 gttgttcctg aacgctgttc atactcacac gtatgctttg caatcccgcc ttgcatagtt
     7801 cttcaatggc ttcgggtttg ctggcattgg tattcaggtt gataatgcct ttatgagttt
     7861 ttgtccgtat cagttttatg gcatcacgca aagtttccca aaccaacagc ggttctccct
     7921 cacaaccctg cccgaagctg acgaccggat tgggcgcggt gttgaggtgt tccaccgcaa
     7981 tctcagcaat ttcttcaggt gtcggaataa aggtgatgcg gttttgtgta gaagtgatcc
     8041 aatggtcttt aggctgatac gagatacaac ctacgcaacg ggagttacag ctaggcgacg
     8101 tcggcagcgg cgcttcccag cggttcataa aatagttgcg ggccgcagga caaaaatata
     8161 ccagcgcaca atgagaaagg tgcttcagca aacgattagc cgggttggcc ttgagcatac
     8221 gtttggcatt tttctccaca taaaactgat tgaa
//
LOCUS       DAOPYB010000139         8214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_10419_length_8214_cov_7.769334, whole genome shotgun sequence.
ACCESSION   DAOPYB010000139 DAOPYB010000000
VERSION     DAOPYB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10419_length_8214_cov_7.769334"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PKH58_09945"
     CDS             <1..572
                     /locus_tag="PKH58_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89395.1"
                     /translation="LVENTRNTRDSIENVELKQRITVSEKELNDAKIQLKINEQKASE
                     LIERLNISETEKQQLRETFETIVKEYNDQGVLIKESYSKRTSELIKDISKLEEQNKNL
                     TSTISTQTELITHYTSEITRVSDTNVELNKKVQFLEKENKEIKEKSVSKPVFQWWLIV
                     IGFIAGIVAYYYLGGVIGKVVSLVLKIIK"
     gene            complement(653..1807)
                     /locus_tag="PKH58_09950"
     CDS             complement(653..1807)
                     /locus_tag="PKH58_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNX89396.1"
                     /translation="MITPKFYLVKKANGTATVYVYFYINREKVNFSTKIICEPANWDD
                     NKMCLKKSDRDYSDKNIVIESIRSRINNVFVKYRLKDRKITREIFLKNYNKPSDYDTF
                     HGYCEVVIRRTRSKLEVNTYRMHKSVLKKLKEYNSTLHFDDIDGEFIENYKRYLTQKL
                     ENNLNTTYKNLAVIRKYVRSAIREGYIEINPFAEISVNRCKTNIVFLTDDELKKLCDE
                     YNSKNYEEKHKTTFQCFLFMCFGSQHIGDARAMKIEQFGPESFTYFRKKTRNRKPEPV
                     TVPISTSLRKLVNELCDGREKGYLLQKLPAEQTMNEYLKTIADNAGISKKISHKTGRH
                     TFATIYLQNNPNLKLLQEILGHSDMKTTMMYVHAMEKYASSGIDCFNKFL"
     gene            complement(2034..2444)
                     /locus_tag="PKH58_09955"
     CDS             complement(2034..2444)
                     /locus_tag="PKH58_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX89397.1"
                     /translation="MRVNNQVISKLLKSYKNEKDFTFDELGSELGISSRTCKSIINDL
                     TNVSLETLIKICTIFRKDINTFIIYEESDEIKETDSVGIKITDGIEYILDRFETLASE
                     KGKVEGELERYKAFFGELPEVDIAAEPSQKFKKK"
     gene            2561..2773
                     /locus_tag="PKH58_09960"
     CDS             2561..2773
                     /locus_tag="PKH58_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89398.1"
                     /translation="METKTKTFNTKINFADYYNSLSDEDKNAIRYKMCPVYMAESTFF
                     YKVRENKFNPLEIEKLTVITGIEFEK"
     gene            2770..3321
                     /locus_tag="PKH58_09965"
     CDS             2770..3321
                     /locus_tag="PKH58_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX89399.1"
                     /translation="MKDIEFYTRPDGEVEICEAGKPSYQLTEDRDRDFIVKMIIKIRE
                     EYPEAFKALTDIYFSNINNRTYYDFLTVKRFIKCNWSQFDNTSDIDKEGNLHFEFTCC
                     PMRGECKYHKIICEPKHQVSISPAEMNVLRLMVDELNDSEIADSLHISIFTVQNHRKN
                     MMRKYQFRHASQLINLWHKNNLK"
     gene            3350..3649
                     /locus_tag="PKH58_09970"
     CDS             3350..3649
                     /locus_tag="PKH58_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89400.1"
                     /translation="MKIEVTRRISDEPEWISEVWNKVFNMCINCSGAVEDENGRLRTH
                     HAYVDAFLGHNEYSCSISLRAENNDRKIGLFRFDRNTSKDAWGFFDKFGNKLILH"
     gene            3665..3943
                     /locus_tag="PKH58_09975"
     CDS             3665..3943
                     /locus_tag="PKH58_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89401.1"
                     /translation="MIDITLTFTIADAVQVFRNAGLTVEMRQEPFVFPAHGSGEEIHM
                     LDVWVVVNPNTGCSVMLDNIFKQYIELKKRELFLQEDNKLKIINLFRI"
     assembly_gap    4113..4212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4213..4431
                     /locus_tag="PKH58_09980"
     CDS             <4213..4431
                     /locus_tag="PKH58_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89402.1"
                     /translation="GDKSLVEYSRKLANFIYNLPDNTEIVIDKIVKPDTEDLFAKCIC
                     RFNVEGTFNLQFSADFTRLRKYELTKNH"
     gene            4460..4855
                     /locus_tag="PKH58_09985"
     CDS             4460..4855
                     /locus_tag="PKH58_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89403.1"
                     /translation="MRTNWKHHEQYVRQNIDKKSVQEMADHLGVKPYDLDQFIHRERI
                     FSVNTTIKNRAYEVVKKKFVHPEYFKPNRNFFRAIGMSQRQWWAVYRGVYPISDEHYL
                     RLTEHLGLTLPEAIELKQISINFPAGSAQ"
     gene            4852..>5648
                     /locus_tag="PKH58_09990"
     CDS             4852..>5648
                     /locus_tag="PKH58_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HNX89404.1"
                     /translation="MNYTKENTERIQQAAEGRLLDVITKQVELKKKGASYKGKCPVCS
                     KTSFEYHDGKKIFKCFKCDFAGNSPVSFHMKQGKTYPEALQALADQFNVYIDEPVRST
                     KKVSKKAPAKPADPEQKEPAEAAPKSSARVTTYCARMLAESGLTTDDVQAKVFYKDLN
                     RTTTISHVFKSGTVNSLNSIVDGDDVIIEYYDLEGAPVMYEQMLKGKSTGKLKEYFRV
                     RWQFPDEHLDKNGKPFKYKSPTGSGSFIYIPQVIRDIYKSGGKIERL"
     assembly_gap    5649..5748
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5749..7770
                     /locus_tag="PKH58_09995"
     CDS             <5749..7770
                     /locus_tag="PKH58_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNX89405.1"
                     /translation="AIQLKNSRSIYLEIYIGNVQPNERDDKGIDDLLANTLRGHEEDL
                     KADIDYLINERSLTGRFVQLYKITTWPDSKIQELWSLHNASEFAQRHKELLKQLPEFR
                     IGKYRWRFNEKGEIESTQPVESEEKYWDEIIKEDRNGNQRTEYRFRYERCFRFLQNRG
                     FYRFRAPSGDYEYIRIEHPVISTIERHEEIRDFVKDFTREIANEEVLEMLHRGGPQFL
                     GPEKLSNLAFNNNIMFEEPRRDKQLLYFKDNYWEIRADEITVGQYSSITHQLWKDQIH
                     DMNAARTERLIEVEKINDKYFNYRLSFTGETCHFLKFLINTSNFTWRKEKNGAEIVDS
                     AEGLDESKVITRQEIMENKGHLVSKLCAIGYMLLSAKDRSVSKAVVAMDGKQSEVGKS
                     NGRSGKSLIGELFKQVMPAIYINGKHKDIEGDNFLWDELTLKTKVVFIDDVRTNFPFE
                     FLFANITGDWSVNYKGNRRATFPFQQSPKIYMTTNHALNGEGSSFSDRQWKIAFSDYY
                     NDEHKPVDDFGTLFFDEWDFEQWNLTWNLMAECIRLYLRFGVVEAPSERIELRQLRQF
                     ISEGFLMWADEYFSDQEHFGTRIPRKVMQEAFFENSPDQRKWVTPTLFKAKIRKYCEF
                     KGYLFNPQKYDPVTGLCMFLDKDGNPDDDDKSGGVEFFTLGYKEDDKPF"
     gene            7792..>8214
                     /locus_tag="PKH58_10000"
     CDS             7792..>8214
                     /locus_tag="PKH58_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89406.1"
                     /translation="MKELPVLFSTPMVQAILENRKTMTRRTHGLERINDYVQKGHTFR
                     YDSIEDGPDEDGNIWFEEISPDGNPTGIYRSAKPQYQIEDHIWVRETFFDARKWKYAP
                     LFADGPDFIYKADEDAFIGDHNWKPSLFMPKEAARIWLE"
BASE COUNT         2535 a         1651 c         1642 g         2186 t
ORIGIN      
        1 tattggtgga aaacacccga aacacccgtg acagcatcga gaacgtggag ctaaaacagc
       61 gcataaccgt ttcggaaaaa gagctgaacg atgctaaaat tcagctgaaa atcaacgaac
      121 aaaaggcaag cgaactgatc gaacggttga atatctccga aacggaaaaa caacaactcc
      181 gggagacgtt tgaaacgata gtgaaggaat ataacgatca gggagtgctg atcaaagaaa
      241 gctactcaaa gcgcacttcg gagcttataa aagacattag caagctggaa gagcagaata
      301 aaaacctcac atccaccatc agtacgcaaa cggaactgat cacccactat acctccgaaa
      361 tcacccgtgt ttccgacacc aacgtggaac taaacaaaaa agtccaattt ttggaaaagg
      421 agaataaaga aataaaggaa aaaagcgtca gcaagccggt ttttcagtgg tggttgattg
      481 tgattggttt cattgccggt attgtggcgt attactatct gggcggagtg atcgggaagg
      541 tggtgagttt ggttttgaaa ataataaaat aagaatactt tttcaggtat tttttggttt
      601 aggaatttgt gtgtgacgac atcccctggt tgtgaaatca ggggatgttt tgtcatagaa
      661 atttattgaa acaatcaatt ccggaactgg cgtatttttc cattgcgtgc acatacatca
      721 ttgttgtttt catatctgag tggccgagaa tttcctgcag gagttttaag ttgggattgt
      781 tttgcaggta tattgtagca aaggtgtgtc gtccggtttt atggcttatt ttttttgata
      841 ttccggcgtt atcggcaatc gtttttaaat actcgttcat tgtttgttct gccggtaatt
      901 tttgcagcaa ataacctttt tcccgcccat cacaaagctc attaactaat tttcgtaagc
      961 tggttgaaat cggcactgtc accggctccg gttttctgtt gcgtgtcttt tttctgaaat
     1021 aagtaaaact ttcaggtccg aattgttcta tcttcattgc tcgtgcatcg cctatatgct
     1081 gactgccgaa acacataaag agaaaacatt gaaaagtggt tttatgcttt tcttcataat
     1141 tctttgagtt gtattcgtca caaagtttct tcagctcatc atcagtaaga aaaacgatgt
     1201 ttgttttaca cctgtttacc gaaatttcgg caaacggatt tatttcaatg taaccttccc
     1261 gaattgcgga tctaacgtat tttcgaataa cggcaagatt tttatatgtc gtgtttagat
     1321 tattttccaa cttttgtgtc aaatatcttt tataattttc gataaattca ccgtcgatgt
     1381 catcaaaatg cagtgttgaa ttatactctt tcagtttctt aagtacactt ttatgcattc
     1441 gataagtatt tacctcaagt ttgctccttg ttcttctaat aacgacttca caataaccgt
     1501 gaaaagtgtc atagtcgctt ggtttattgt aattcttcag aaatatttcc ctggttattt
     1561 tccggtcctt caggcgatat tttacgaaaa cattgtttat cctggaacgg atactttcaa
     1621 taactatatt tttatcagaa taatcccgat cgcttttctt taagcacatt ttattgtcat
     1681 cccaatttgc aggctcacaa ataattttag tagagaaatt gactttttct ctattgatat
     1741 aaaagtaaac gtaaacggta gccgttccat ttgctttttt tacgaggtaa aattttggcg
     1801 taatcatata tacatttagt ctataatttt aattttttat acacgtagtc tgcatttttg
     1861 tacgaatgtt caaatttttt cccaagaact tgcttaccca acttgttgaa agtcaataaa
     1921 aacgcaaatg cccacgaccg tatgatcgtg agcattttgt taagaggtgg taccaccaga
     1981 agtcacgacc tatttagtta ttagttagtt atgatttatt aggatacaat aatctacttc
     2041 tttttgaatt tttgtgaagg ttctgctgca atatcaacct cagggagttc tccaaaaaat
     2101 gctttatatc gctctaattc cccttccact tttccttttt ctgatgctaa tgtttcaaac
     2161 ctgtcaagaa tatattcaat tccatccgtt attttaatgc ctaccgaatc cgtttcttta
     2221 atttcatccg attcctcata aattataaat gtgttaatgt cttttctgaa aatcgtacat
     2281 atcttaatta gagtttctaa tgaaacgttt gttaaatcat ttataattga tttacaagtt
     2341 cgactactta ttccgagttc tgaacctaat tcgtcaaaag tgaaatcttt ttcatttttg
     2401 tagcttttta gtaatttgct tataacttgg ttgttaactc tcataatatt tagatttatt
     2461 ctaaatttat ataattagtg caaaataaaa gtgaaagaac ttgcactttt agtgaaatta
     2521 aatttacttt gcgactataa atttaatagt aaatattata gtggaaacaa aaacaaaaac
     2581 atttaacaca aaaataaact ttgcagatta ctacaatagt ttatcggatg aggataaaaa
     2641 tgccataaga tacaaaatgt gtccggttta tatggccgaa tctactttct tttacaaagt
     2701 gagagaaaat aagtttaatc ctcttgaaat agaaaaactt accgttatta cgggaataga
     2761 atttgaaaaa tgaaagatat agaattctat acacggccag acggtgaagt ggaaatttgc
     2821 gaagccggaa aaccatctta ccaacttaca gaagatcgag atagagattt tattgttaag
     2881 atgattataa aaattcgcga agaatatccg gaagcattca aggcattgac ggatatttat
     2941 ttttccaata ttaataatcg tacgtactat gattttttaa cagtaaaacg atttattaaa
     3001 tgtaactgga gtcaatttga caatacctca gatattgata aggaaggaaa tctacatttt
     3061 gaatttactt gttgccctat gcgtggcgaa tgcaaatatc acaaaatcat ttgcgagccc
     3121 aagcaccagg tatcaatatc acctgcagag atgaacgttc tacgtctgat ggtagatgaa
     3181 ctcaacgaca gtgaaatagc tgatagtctt catataagca tttttacagt tcaaaatcac
     3241 cgtaaaaata tgatgcgtaa atatcaattc agacacgcct cacagctgat aaacttgtgg
     3301 cataaaaata atctgaaatg atgactaacc tctcaaaaat ataattatta tgaaaatcga
     3361 agtaacaagg cgcataagcg acgaaccgga atggataagc gaagtttgga ataaggtttt
     3421 caatatgtgt attaattgtt ccggagcggt ggaagatgaa aatggacgac ttcgcaccca
     3481 tcacgcgtat gtggatgctt ttttaggaca taatgagtac tcctgctcaa ttagcctccg
     3541 ggcggaaaac aacgacagga agataggttt gtttcgtttc gaccgcaata cgtcaaagga
     3601 cgcgtggggc ttttttgaca aattcggtaa caaattaata ttacactaat aatgataaat
     3661 catcatgatt gatataacgc tgacttttac aatagctgat gccgtgcagg tgtttcggaa
     3721 cgcaggactg accgttgaaa tgcgacagga accttttgtt ttccctgcac atggaagcgg
     3781 cgaagaaatt cacatgctgg atgtttgggt ggttgtaaat ccgaataccg gatgttcagt
     3841 aatgcttgac aatattttca aacagtacat cgaactgaaa aaacgggaac tattcctgca
     3901 ggaagataat aaactaaaaa tcattaacct ttttaggata taactatgcc gaagtacaac
     3961 acattcaccg gtaacggatt tgaaatgaaa tcatttcacc gtcggccaac aacgaactca
     4021 acccggcaca gctgggttaa cgactgctgc caaaactgcg gaattacccg caccaaagat
     4081 atcgaaaaag gaattataaa aacgtactac acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnggcgataa atcactggta gaatacagcc gcaagctggc taacttcatt
     4261 tacaaccttc cggacaatac agagatcgta atcgataaga ttgttaagcc tgatacggaa
     4321 gatttatttg caaaatgcat ctgccggttc aatgtagaag gaacattcaa cctgcagttc
     4381 tcggcagatt tcaccagatt aagaaaatac gaattgacta aaaaccatta aatcattcca
     4441 tcatctttaa atttgaatta tgcgcacaaa ctggaaacat cacgaacaat acgttcgtca
     4501 aaatatcgat aagaaatcag tacaggaaat ggccgatcac ctgggcgtca aaccatacga
     4561 cctcgatcag ttcattcacc gtgaacggat attctccgtc aataccacta tcaaaaacag
     4621 agcatacgaa gttgtcaaga aaaaattcgt acatccggaa tacttcaaac ctaaccggaa
     4681 tttcttccgg gctatcggga tgtcacagcg ccagtggtgg gctgtctaca gaggcgttta
     4741 tcccatcagc gacgaacact acctccgact taccgaacac ctcgggctta cactcccaga
     4801 agcaatcgaa ctcaaacaaa tatcgattaa ttttccagcc ggatccgcac aatgaattat
     4861 acaaaagaaa atacagaacg cattcagcaa gctgctgaag gccgtctgct cgacgtcatt
     4921 actaaacagg ttgaactgaa gaaaaaagga gcgtcttaca agggaaaatg tccggtatgc
     4981 agtaaaacat ccttcgaata ccacgacggt aagaaaatat tcaagtgctt taaatgtgat
     5041 tttgcaggta attctccggt aagtttccac atgaagcagg ggaaaactta tcctgaagca
     5101 ctgcaggcgc tggccgatca gttcaacgtt tatatcgatg aaccggttcg cagcacaaaa
     5161 aaggtgtcca aaaaagcacc cgcgaaaccg gctgatccgg aacagaaaga acctgctgaa
     5221 gcagcgccta agtcatccgc cagggtgaca acttattgcg cccgcatgct ggccgaatcg
     5281 ggacttacca ccgatgatgt tcaggctaaa gtattttata aggatttgaa ccgcacgact
     5341 accatcagcc acgtcttcaa gtccggaacg gtgaattcgc tgaacagcat cgtcgatggc
     5401 gatgatgtga ttatcgagta ctacgatctc gaaggtgctc ccgtgatgta tgagcagatg
     5461 ctgaaaggga aaagcaccgg aaaactgaag gaatacttcc gcgtgcgctg gcagttcccg
     5521 gacgaacacc ttgacaaaaa cggtaagccg tttaaatata aatcacctac cggctcaggt
     5581 tccttcattt acattccgca ggtcatccgg gatatttata aatcaggcgg aaagattgaa
     5641 cgattattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cattcagctg
     5761 aaaaattccc gctctattta cctggaaatc tacatcggaa atgttcagcc gaacgaaagg
     5821 gatgataagg gaattgacga cttattagcc aatacgctgc gaggtcacga agaggatctg
     5881 aaagcggata tcgattacct catcaacgaa cgctcactca cgggccggtt cgttcagctt
     5941 tacaaaataa ccacctggcc ggatagcaaa atacaggaac tgtggtcact ccacaatgcc
     6001 agtgaatttg cacagcgaca caaggaattg ctgaaacaac tgccggaatt ccgcatcggc
     6061 aagtatcgct ggcgctttaa tgaaaaaggc gagattgaaa gcacgcagcc agtggagagc
     6121 gaggaaaaat actgggacga aataattaag gaggaccgta acggcaacca gcgcactgaa
     6181 taccgcttcc ggtacgaacg ctgtttccga ttcctgcaaa acaggggctt ctaccgcttc
     6241 cgcgctcctt cgggcgatta tgaatatatc cgcatcgagc atcctgtgat cagcacaatc
     6301 gaacgtcacg aggagatccg ggactttgtt aaggatttta cccgcgaaat tgcaaacgag
     6361 gaagtgctgg aaatgctgca ccgcggaggt ccgcagttcc tgggaccgga gaaactctcc
     6421 aaccttgctt tcaacaacaa tatcatgttc gaggaaccgc gccgcgataa acagctgctg
     6481 tatttcaagg acaattactg ggagatcaga gcggatgaaa tcaccgtagg gcagtactcg
     6541 tccattacgc accagctttg gaaggatcag atccacgaca tgaacgccgc ccgcacggaa
     6601 cgcctcatcg aggtggaaaa aataaacgac aagtatttca actaccgcct gagcttcacg
     6661 ggcgaaacct gccactttct gaaatttctc atcaatacat cgaatttcac ctggcgaaag
     6721 gaaaagaacg gagccgaaat agttgattcg gctgaaggac tggacgaaag caaagtgatc
     6781 actcgtcagg aaatcatgga aaacaaggga cacctggtat caaagctctg tgctatcggt
     6841 tacatgctcc tgagcgctaa agaccgctct gtaagtaaag cggtagtggc aatggatgga
     6901 aaacagagtg aagtgggtaa aagcaacgga cgctccggta aatcgctgat tggcgaactg
     6961 ttcaaacagg taatgccggc aatttatatc aatggcaaac acaaggatat cgagggcgac
     7021 aacttcctgt gggatgaact tacgctgaaa acaaaggtgg tattcatcga cgacgtgcgc
     7081 accaatttcc cgttcgaatt cctgttcgcg aacattaccg gtgactggtc tgtcaactac
     7141 aagggtaatc gccgtgccac atttccgttc cagcaatccc caaagattta catgactact
     7201 aaccatgccc tgaacggtga aggctccagc ttttccgacc gtcaatggaa aatagccttc
     7261 agcgattatt acaacgatga gcataagcct gttgacgatt tcggcaccct cttttttgac
     7321 gaatgggatt ttgaacaatg gaacctcacg tggaacctga tggctgaatg tattcggctg
     7381 tatctccgct ttggcgtggt ggaagctccg agcgaacgca tcgagctgcg ccagctccgc
     7441 cagttcattt cagaaggttt cctcatgtgg gccgacgagt atttctccga tcaggaacat
     7501 ttcggcacgc gcatcccgcg caaggtcatg caggaagctt tcttcgaaaa cagccccgat
     7561 cagcgcaagt gggttactcc aacactcttt aaggcgaaga tccgcaaata ctgcgaattc
     7621 aagggatacc tcttcaaccc gcagaaatac gacccggtaa cagggttatg catgttcctc
     7681 gacaaagacg gaaacccgga cgacgacgac aaatctggtg gagtggaatt cttcacatta
     7741 ggctacaaag aagacgataa accattttaa aacaactaat aaacaaaaac catgaaagaa
     7801 ctacctgttt tattctctac accaatggtt caagccattc tggaaaaccg taagacaatg
     7861 acccgcagaa cgcacggact tgaacgaatt aatgactatg ttcaaaaagg acatacattc
     7921 agatacgaca gtatcgaaga tggtccggat gaagacggaa atatctggtt tgaagagatt
     7981 agtccggatg gaaatccaac tggaatatat cgcagcgcaa aaccacaata tcagattgaa
     8041 gaccacattt gggtacgcga aacgtttttt gatgctcgca aatggaaata tgctccatta
     8101 tttgccgatg gtcctgattt tatttataag gccgacgaag atgcttttat cggagatcat
     8161 aactggaaac catcactgtt tatgcccaaa gaagctgccc gtatttggtt ggaa
//
LOCUS       DAOPYB010000140         8044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_10733_length_8044_cov_7.705720, whole genome shotgun sequence.
ACCESSION   DAOPYB010000140 DAOPYB010000000
VERSION     DAOPYB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10733_length_8044_cov_7.705720"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PKH58_10005"
     CDS             complement(<1..593)
                     /locus_tag="PKH58_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNX89407.1"
                     /translation="MKKILIFSILLSLSFSGLAQSPKREFRATWLTTVWRNDWPKSTI
                     ITSTGNSGEIQMQKNEMIAILDSLKRLNANSVCFQVRSRCDAMYKSSYEPWSSDLVGT
                     RGMDPGYDPLAFVVQEGHKRGIEVHTWINPYRFESVTNQWSGLPGDYRSTHPDWLLTY
                     PATNNSILNPGHPEVLLQIKKIVAEIVKNYDIDGVIF"
     gene            complement(600..>1415)
                     /locus_tag="PKH58_10010"
     CDS             complement(600..>1415)
                     /locus_tag="PKH58_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX89408.1"
                     /translation="DGRIMVMSKDGAKVETMMTNTGAAFNDPYYGYIEGGQLYFSDRN
                     TGFATIGLKERNRTFSRTDYPYYVQNATLGYYGQGISYGAMNAGFGKINNVWYWCKTY
                     NGNGIFRFTDADILKIPITGNGVLPSSGIALESMTPKSLTWDAKNNVIYFTLYDTGYE
                     GLYRCTLAQLNEIGGNKEKLAPYKLKLANGKTVTPVTETGKGEGSTGEFIAICQLALD
                     ENDGCVYFGLRSGDAAVKSGLMRYNPATGFIEYVVEGVEVYGVAVNKTKSKLF"
     assembly_gap    1416..1515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1516..2102)
                     /locus_tag="PKH58_10015"
     CDS             complement(<1516..2102)
                     /locus_tag="PKH58_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX89409.1"
                     /translation="MKIIHYFLSLFIALIFFSCVDNDPVIEELPNPDIAFNYSVIDTE
                     YQIDYYVGANIEFVSSSVAPGECSWDFGDGTMATGETVTHKYKKAGTYTVKLTIAGAG
                     YQSKPILISDIKPIVSPNKTGLIEVVSTSVGFNVELPNPEKLTEEYHWIFPDGTLDAA
                     GNPIATFDGKNPGNVTFSNIGSQTVTLQVKLGGRQL"
     gene            complement(2118..2966)
                     /locus_tag="PKH58_10020"
     CDS             complement(2118..2966)
                     /locus_tag="PKH58_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89410.1"
                     /translation="MKTKYNIIFMMAISLLFIACEKHDMMDNDVIVGQMAPQVYWEPA
                     SSTVKAGENVPFMVQYYTTTTENIDRLEVWYNIIEDEAKAVSSPWVSTFTYSVTSNKS
                     LEKRVSQKISTYAHEPNSWNDSVRAFMFKTTFPTSNTLASVTWSKPGTFDNDKMVKYF
                     GTTFMTQFKDSLFKLMKPKDFQKMYLGLNLLDNFKIYIDSVKNENTGGWDYVFPKDLQ
                     GNRTVPKTLVDLYATIPFSDIIFNKTTNVYDVDYSRSYRINANIKAFDKKGVAGLGIV
                     KEISLN"
     gene            complement(2986..4752)
                     /locus_tag="PKH58_10025"
     CDS             complement(2986..4752)
                     /locus_tag="PKH58_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX89411.1"
                     /translation="MKTYRIFLTILISSVFLFNSCQDFFDVNTSKDTPSEVSPEQLLP
                     VLVFYSSQLCYDHAEYGVYLSQALTTGGRSQTSAYAYKAGWELLSMNRHPQWRRHFYD
                     IGVNADALIKGAEKIDSRNYILIARTIRLQSTLFTTDAFGDMPLSQVYQSNSPSYDTQ
                     ESVYEWMYKEADELIALYDNPEWISYKNNRTISVLQDRLFGGDMEKWRAYAKALKARL
                     LLRMIPNWKNTPENCQKIMDAADAALNDPSYTDALYRYDGGSTEKNCPWGPAQPKLNL
                     GWAQARENLLTDAIPSKFFAYALLSVYASPRQTIGMSLDPRAEQLMTPRLDDKNANTI
                     RYIENNIGMPATMKITYYPDLYALQGGNPYTRNNGYIMLMSKEELLFIKAEAQYWKGD
                     KEGAYNTTVDAVKLNMRRVGVKMDDPNMKPFIDRFFSIRLPGAATFTIADLMQQKYVA
                     MYLQPELWTDVRRYNYSSKNNGIAYDGVFVYHVTRIHDGTRNVNPTNFSQEYSLTRPF
                     NLYAAYWIDKPDNFGVNAKFSPNAWITRLNYDPETEDKYNRKELERLGAYKNPEWLRK
                     RMIWAYKNNNFVTSADPDIEWK"
     gene            complement(4764..7886)
                     /locus_tag="PKH58_10030"
     CDS             complement(4764..7886)
                     /locus_tag="PKH58_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX89412.1"
                     /translation="MKKFYFLTLLSLLIFSPILAQNIVTGVVTSSDDQLPVIGVSVAV
                     KGTNRGVLTNVDGRYSISASGNETLVFSFVGMEKQERRVGNQTVINVVLNPTTEMLEE
                     VVVTAMGVKTEKKKLNFAVQTVNADALTEGRQANFINGLLGKVSGINVTNSGGSPNSG
                     SLLIIRGISSINPSQSNAPLYVLDGVAISGGVSDINPNDIDNITILKGAAASALYGQS
                     GANGAVLITTKQGVTGKISTNVNASVQTNQPIRTPAQQTMYGPGALGFYKPEASGGYG
                     GWGPLLQPDETRYDNIKNYFQTGLYQKYDINVSGGSEKVKAYASAYYSRDKGIVVNDY
                     LNKIGILAKGSYDITKKITLSAMANIVNDVSRGAGGVSSAYSWPINDDITNYMEGGKV
                     RYRYISEKLKSASPVSPLFSRYMDYGKNDNLRAIMQGAINYKAFKNFEAIGRVNFETN
                     SYLYDGYAVPRFDDAVILPDPPKADQFGENTDAYAKALAGYNTMYYQSPYFNQSDYAN
                     VDKDILGNYAHSNSRSQFVTLMGILTYKINLISDLSLDLLAGGEVKTRTGISSSIAGR
                     DFIVPGIYSLENVREIQGVNDIGVSHTQRRNGGIFDQVTLDYKGLASLSATTRWDWSS
                     TLSQEFSPYFYPSVTAGVLFSELLNVKNKWFNYGKLRGNWAEVGKDTPAPYLFDRKYT
                     QYPTLPDGGYGVDPSKSVASLLRPEMSSSWEIGLETRFFNSKTRFDLAYYSTYVDNQI
                     VTVRVSPASGYILQTRNEGTVENHGMEFLWDQDILSQRDFSWKTSLTFGFNRGKVVKL
                     PDDIVEIQGTQYTDVFPTAYLNGSTTAISGKDYERTEDGKVIVNAEGYPKINPTKSVL
                     IGNREPDFQMGLNNTFYFRGLNLAFLIDGRKGGDIYNATSRGMWSSGQHKDLEFYRGR
                     QVVWDGVVKQPDGSYIKNTTPIVLDSKTITEYYAGVSSNFIEDGSYIRLNYVTIGYDF
                     LSLLKKKTQFTSLKCSLTASNLLLLTKYTGSDPQINANTGSGGTGGMGIDSYPVPPTR
                     GINFTVLATF"
BASE COUNT         2086 a         1773 c         1651 g         2434 t
ORIGIN      
        1 tcaaaaataa ctccgtcaat gtcgtagttt tttacaattt cagcaactat tttttttatt
       61 tgaagcaata cttcaggatg tccgggattt agtatggaat tgttggtggc cggatatgtt
      121 aacagccagt ccggatgtgt gctgcgataa tcgcccggca gtcccgacca ctgattggtt
      181 accgactcga accggtaggg attgatccag gtgtgaactt caattccccg tttgtgtcct
      241 tcctgcacta caaatgccag tggatcgtaa ccggggtcca tgcctctggt gcccaccaga
      301 tccgatgacc agggttcgta ggatgattta tacatggcat cgcaccgtga acgcacctga
      361 aagcacaccg aattggcatt cagtcttttc agtgaatcca atattgctat catttcattt
      421 ttttgcatct gaatttcacc cgaatttccg gttgatgtaa tgattgtgct tttaggccag
      481 tcgttacgcc acacggtagt gagccaggtt gcccgaaatt cccgtttggg tgattgtgcc
      541 aatccactaa aacttaagga aagcaatatt gagaatataa gaattttctt catccggttt
      601 tagaataatt ttgatttggt tttattgaca gctacaccat aaacttccac tccttctact
      661 acgtattcga taaaaccggt tgccgggttg taacgcatca atccggattt aacagcagca
      721 tcgcccgagc gaagtccgaa ataaacacag ccatcatttt catcgagagc caactgacag
      781 attgcaataa attcgccggt tgaaccttcc ccttttccgg tttcggtaac cggagttaca
      841 gttttcccgt tggctaattt gagtttgtaa ggtgccagtt tttccttgtt gccgcctatt
      901 tcattgagtt gagctaatgt acaacggtat aatccttcgt atccggtatc gtacaaagtg
      961 aaatagatga cattattttt tgcatcccat gttaacgatt tcggagtcat gctttcgagt
     1021 gcgatgcccg atgagggcaa tactccgttg ccggtaatcg gaattttaag aatatcggca
     1081 tctgtaaacc ggaaaattcc gtttccgttg taagtcttgc accagtacca cacgttgttt
     1141 attttaccga aaccggcatt catggcacca taactaatgc cctggccata atatcccaag
     1201 gtggcattct gaacataata cggataatca gtccggctga aggttctgtt tcgctccttc
     1261 aatccaatgg tcgcgaagcc ggtatttctg tcagagaaat acagttgtcc gccttcaata
     1321 tatccgtaat acgggtcatt gaaagcagca cctgtgttgg tcatcatggt ttccactttg
     1381 gctccgtctt tcgacatcac catgatgcgc ccatcnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnagctg cctgccgcca agttttacct gcagtgtcac ggtctgagaa
     1561 ccgatattgc taaacgttac atttcccgga tttttgccat cgaaagtggc aatggggttg
     1621 ccggctgcat ccaacgttcc gtcggggaaa atccagtgat attcttcggt taatttttcc
     1681 ggattgggca attccacatt aaatcctacg gaggtggaaa cgacttcaat cagaccggtt
     1741 ttattgggcg atacgatggg tttgatatca ctgattaaaa tgggttttga ctggtatcct
     1801 gcacccgcta tggtcagttt cacggtgtag gtgcctgctt ttttgtactt gtgagtcacg
     1861 gtttcaccgg ttgccattgt tccgtcgcca aaatcccagc tgcactcacc gggtgctacc
     1921 gaagaactga caaactcgat attggctccc acataatagt caatttggta ttcggtatca
     1981 atcactgagt agttaaaagc tatatccgga ttgggtaatt cttcaataac cgggtcattg
     2041 tccacgcagg aaaaaaatat cagcgcgatg aatagcgaca aaaagtaatg tattattttc
     2101 ataaccgtcg ttttatttta attgagtgag atttccttta caattcccag accggcaact
     2161 ccttttttgt caaatgcctt aatatttgca tttattcggt acgaacgcga ataatccaca
     2221 tcatatacgt tggtggtttt attaaaaatt atatcggaaa aaggaatagt ggcatacaaa
     2281 tcaacgagtg tttttggaac ggttctgttg ccttgtaaat cttttggaaa tacataatcc
     2341 catccgccgg tattctcatt tttcacagaa tcaatgtata tcttaaaatt atccagcaaa
     2401 ttcagtccta aatacatttt ctgaaaatcc ttgggcttca taagtttgaa gaggctatcc
     2461 ttgaattgtg tcatgaaagt ggttccgaag tatttcacca ttttatcatt gtcgaatgtg
     2521 cccggtttgc tccaggttac cgatgccagc gtattactcg ttgggaacgt ggtcttaaac
     2581 atgaatgccc gtaccgaatc attccaggaa ttgggttcgt gagcataagt cgatattttt
     2641 tgtgaaactc ttttttcaag tgacttattg gaagtaacgc tataggtaaa agttgatacc
     2701 cagggacttg acactgcttt tgcttcatct tcaataatat tgtaccaaac ctccaggcga
     2761 tcaatatttt cggtagtggt agtgtaatac tgaaccataa aaggtacatt ttcaccggct
     2821 ttgactgtgc tgctggcagg ctcccaataa acctgcggcg ccatttgacc cactatcaca
     2881 tcgttgtcca tcatatcatg tttttcgcag gcaataaata agagacttat tgccatcatg
     2941 aaaattatat tgtatttcgt tttcatatct tattcattta caggtttatt tccattcaat
     3001 atcgggatcg gccgaggtaa caaaattatt atttttataa gcccatatca ttctttttcg
     3061 caaccattcg ggatttttat aggctcctaa ccgttccagt tctttacgat tgtatttatc
     3121 ttcggtttca gggtcatagt ttaagcgggt gatccatgcg ttaggcgaaa attttgcatt
     3181 tacgccaaaa ttatccggtt tgtcaatcca gtaagctgca tacaggttaa agggtcttgt
     3241 cagtgaatat tcctggctga aattggttgg attaacgttc cgggttccat cgtgaatgcg
     3301 tgtaacatga tacacaaata ctccatcata agctattccg ttatttttgc tgctgtagtt
     3361 gtaccttctc acgtcggtcc acaattcggg ttgaaggtac atggccacgt acttttgctg
     3421 catcagatca gcaatggtaa aggtggctgc acccggcaat cgaattgaga agaaacgatc
     3481 aataaacggc ttcatattgg ggtcatccat tttcactccc acccgtcgca tattcagttt
     3541 tactgcatct acggtggtat tgtaagcgcc ttccttgtct cctttccagt attgagcttc
     3601 ggctttgata aacaggagtt cttctttgga catcagcatg atgtaaccgt tgttccgggt
     3661 atagggattg ccgccctgta aagcgtacaa atccggatag taggttattt tcatggtggc
     3721 gggcataccg atgttatttt caatataccg gattgtattt gcgtttttat cgtccagacg
     3781 gggtgtcatc agttgctcgg cgcgcgggtc tagcgacatt ccgatggtct gtcgcgggct
     3841 ggcatatacg ctcagcagcg cataggcgaa aaacttggac ggaatggcat cggtaagcag
     3901 attttcacgt gcctgcgccc agcccagatt aagtttcggt tgagccggtc cccaggggca
     3961 gtttttttcg gtagagccgc catcgtacct gtagagtgcg tcggtgtacg acgggtcatt
     4021 caatgctgca tccgcagcat ccattatttt ttgacaattt tccggagtgt ttttccagtt
     4081 ggggatcatt cggagtaaaa gtcgcgcttt cagtgccttg gcataagcac gccatttctc
     4141 catatcacca ccaaacagcc ggtcctggag tacggaaatc gtgcggttat ttttatagct
     4201 tatccattcg ggattatcat agagagctat taattcatcg gcttctttgt acatccactc
     4261 atataccgat tcctgcgtat cgtacgatgg cgaattggat tgatacacct gcgaaagcgg
     4321 catatcacca aatgcatcgg tagtgaataa agtcgattgc aaccggatag tgcgtgctat
     4381 gagtatataa tttctcgaat ctattttctc ggctcctttt atcagcgcat ctgcattcac
     4441 tccgatatcg tagaaatgac gtcgccattg cgggtgtctg ttcatagaaa gcagctccca
     4501 gccggccttg taagcgtaag cactggtctg gctccgacct cctgtagtaa gtgcctgcga
     4561 cagataaaca ccgtattcgg catggtcata acacaattgt gatgaataaa aaaccaaaac
     4621 agggagcaac tgctcggggg aaacctctga gggtgtgtcc ttgctggtat ttacatcaaa
     4681 aaaatcctgg cagctgttga ataaaaaaac actgctgatt aatattgtta aaaatattct
     4741 gtatgttttc attgcgattc agattaaaat gtagctaata ctgtaaaatt aattcctctt
     4801 gtaggtggca cgggatagga atcaattccc attcctccgg taccacccga acctgtattt
     4861 gcgttaattt ggggatcaga accggtatat ttggtcaata gtaaaagatt ggaagctgta
     4921 agcgagcatt tcaaactcgt aaactgtgtt tttttcttca acagtgacag gaaatcatat
     4981 cctattgtca cataattcag tcggatatac gaaccatcct ctatgaaatt ggaacttact
     5041 ccggcgtaat attcggttat ggttttactg tcgagtacaa tcggggttgt atttttgatg
     5101 tacgagccat ccggttgctt caccactcca tcccacacca cttgccgacc tctgtaaaat
     5161 tccaggtctt tgtgctgacc acttgaccac attccccgac ttgtagcatt atatatatca
     5221 ccgccttttc gaccgtcaat caggaaagct aaatttaaac ccctgaaata gaacgtattg
     5281 ttcaatccca tttgaaaatc gggttcccgg tttcctatca acacactttt tgtgggattg
     5341 attttcggat atccctcagc atttactatc acttttccat cttccgttct ctcataatct
     5401 tttcccgaaa tggcggtagt ggatccattc agataagctg tagggaacac atcggtgtat
     5461 tgagttccct gaatttccac aatatcatcg ggtagtttaa ccacttttcc ccggttgaag
     5521 ccgaaggtca aagatgtttt ccaggagaaa tcccgctgtg acaggatatc ctgatcccag
     5581 aggaattcca taccgtgatt ctccaccgtt ccttcatttc gggtctgcaa gatatagcct
     5641 gaagccggtg aaacgcgtac tgttacaatc tgattatcga catacgtgga ataataggca
     5701 aggtcaaaac gggttttact gttgaaaaaa cgggtttcaa gtccaatttc ccaggagctt
     5761 gacatctctg gtctaagtag actggccacc gattttgaag gatctactcc gtaacctcca
     5821 tcgggcagcg tgggatattg ggtatatttt ctgtcgaaca gatatggagc cggtgtatct
     5881 tttccaacct ctgcccagtt tcctctcaac ttaccgtaat tgaaccattt attcttgacg
     5941 tttaggagtt cggaaaacaa cactccggct gtcactgaag gataaaaata tgggctgaac
     6001 tcctgactaa gtgtggaaga ccaatcccag cgggtagtgg cgctaagtga tgccagacct
     6061 ttgtagtcga gtgtcacctg gtcaaaaatg cctccgtttc ttcgttgtgt atgtgaaacc
     6121 ccgatatcat tcacgccctg aatttcacgc acattctcca gactatatat gcccggaaca
     6181 atgaagtccc ttcccgcaat ggaggatgaa attccggttc tggttttaac ttcacctcca
     6241 gccaacaaat cgagtgaaag atcactgatg aggttaattt tgtaggtcaa aattcccatt
     6301 aaggttacaa actggcttct tgaatttgag tgagcatagt taccaagtat atctttatcc
     6361 acattagcgt aatcagactg attaaaatag ggactttgat aatacatggt attgtaaccg
     6421 gccaatgctt tggcataagc atcggtattt tcgccaaact gatcagcttt tggcggatcg
     6481 ggcagaatta cagcatcgtc aaaccgggga actgcgtaac cgtcgtacaa ataactgttg
     6541 gtttcaaaat ttacccgccc gatagcttca aagttcttaa acgccttata attaatcgct
     6601 ccctgcatga ttgcacgcag gttatcattt ttaccataat ccatatatct tgagaacaac
     6661 ggacttaccg gtgaagcaga tttcagtttc tcacttatat aacggtaccg gacttttcca
     6721 ccttccatat aattggtaat atcatcgttg atgggccagg agtatgcgct tgaaactcct
     6781 cctgcacctc tggaaacatc atttacaata ttggccatgg ccgacagggt aattttttta
     6841 gtgatatcat aacttccttt ggccaaaatc ccgattttat tgaggtaatc attcaccaca
     6901 attcccttat ctctcgaata atatgcagat gcatatgctt ttaccttttc gctaccaccg
     6961 ctcacattga tatcatattt ttgatacaaa ccggtttgga aataattctt tatattgtca
     7021 taccgggttt cgtcgggctg aagcagtggc ccccaaccac cgtaccctcc acttgcttcg
     7081 ggtttgtaaa atccaagagc tcccggtccg tacatggttt gctgagcagg tgtacgaatg
     7141 ggctgattag tctgaacact ggcattgaca ttggtagata tttttccggt tactccctgt
     7201 ttggtggtaa tcaaaacggc tccgttggct ccgctctgcc cgtacaatgc cgaagcagcc
     7261 gcacctttca gtatggttat attatcaata tcattgggat tgatatcgga aacaccgccg
     7321 gaaatggcca ctccatcgag tacatataaa ggagcatttg actgcgaagg attgatggat
     7381 gaaataccgc ggatgatcag cagggagcct gaattgggtg agccacctga attggtcaca
     7441 ttgatacctg ataccttacc caacagtccg ttgataaaat tggcctgacg accttcggta
     7501 agtgcatcgg cattgacagt ctgtacggca aaattcagtt tctttttctc tgtttttaca
     7561 cccatggctg ttaccaccac ttcttcgagc atttcagttg tgggattaag caccacattg
     7621 attactgttt ggttacctac gcggcgttcc tgtttttcca tgcctacaaa cgaaaaaacc
     7681 aacgtttcgt tgcccgatgc cgaaatggaa taacgaccat ccacattggt cagcacaccc
     7741 cggttggtac ctttcacagc aacacttact ccaattacag gcaactgatc atccgacgag
     7801 gtaacaacgc cggtaacaat gttttgagct aaaattggag agaaaatcaa cagggaaagt
     7861 aatgttaaaa agtagaattt cttcatagaa cttatgtttg taagtttaaa taatattcat
     7921 tcatttttat aacaaccaaa attataaaaa acaattcact taaaccattt ttttagttaa
     7981 aaattattga ataatccgtt aaaatcatcg attcggtcca aatcgctgac attttatcat
     8041 taaa
//
LOCUS       DAOPYB010000141         7957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_10902_length_7957_cov_10.801443, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000141 DAOPYB010000000
VERSION     DAOPYB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10902_length_7957_cov_10.801443"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKH58_10035"
     CDS             <1..185
                     /locus_tag="PKH58_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HNX89413.1"
                     /translation="STTLSFTAASNNFELAIAVAIAVFGINSGEAFAAVIGPLVEVPV
                     MIGLVNVAFWFKNKWF"
     gene            complement(239..2110)
                     /locus_tag="PKH58_10040"
     CDS             complement(239..2110)
                     /locus_tag="PKH58_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNX89414.1"
                     /translation="MILIIIALTFLPELRAQAYSPRQLRNEVLQYQKNQQTKTAASKY
                     RKGGKTQAYQPPPVGATVFKPANPAKYPKSSLIYLENSETLFYDQMLFPDIQVLRGNV
                     RFRHENAVLYCDSAYFNDISNTFDAFGRVKMVQGDTLFIYGDYLNYDGNTRLARLRDN
                     VKLINRNTTLTTDSMNYDRVANLAYYYTGGKVVDDVNVLTSVWGQYSPATRQALFKED
                     VKMTNPNYTITSDTLKYNTKTSVADIVGDSHVLYKEKTNIYSKLGWYDTAKDRMMLLN
                     RSFVEQNDGKTLVGDTIFYDKKNKYSEAYSNVELNDPKQKTTLYGNFVSYDEGKECGL
                     ATDSALFVDWSGKDSLFMSADTLFNFKDSTYDAIKAHKNVRFYRADIQGMCDSLYYTS
                     RDSIMNLKVLPVLWSGSNQLSGDSIKAYTKNQQVEKVHIMQSAMAVQKDTLTYYNQLS
                     GKEITAYLDSGQIRRVDVDGNAESIYFPRDEKTKDIIGLNKTVSSYVKMLFKDKKIER
                     IILTTASSGSMYPIINLEENDLYLRNFYWYEKERPAKSEDVFLKFVRTEPPKRPESNK
                     KPGFPEEKDDSKDKQSSNQAQKNNVNAANRNTGMNRNTGSQNTNNRQRTRNNNMKIQ"
     gene            complement(2171..3526)
                     /locus_tag="PKH58_10045"
     CDS             complement(2171..3526)
                     /locus_tag="PKH58_10045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX89415.1"
                     /translation="MRKISLLLLAFGLLASGLQAQKKNTIDEVIWIVGDEAILKSDVE
                     ALILDAQMRRQPIQGDAYCVIPEQMAIQKLFLHQAALDSVTVNEGYIASLAEADLNKY
                     IADVGSVEKAEEYSHKTVAQLREIIREKYKSQALIEQMQSQLVSGIQATPAEVRRYFS
                     NIPKDSLPTIPEQVELQVLSVNPPIPLSEVNRVKEKLREFTERATANPADFSLLARLY
                     SEDPGSAVKGGELGYTARATLDPEFAAVAFNLQDPKKVSRIVESQFGFHIIQLIDKRG
                     DKVNCRHILLKPRASIEDKQNGITRLDSLADLIRQNKITFEQAVMHYSQDRNTAMNAG
                     LMVNQNTGNSKFEYQDLPSEVAKAAYNMNVGEVSKAFTMIDPLTGKEMITIVKLKSKT
                     PTHKANIMDDYQVLKNIMEGNKKQEFLHNWISKKQKETYISIKPEWRNCNFQYKGWIK
                     E"
     gene            complement(3634..4479)
                     /locus_tag="PKH58_10050"
     CDS             complement(3634..4479)
                     /locus_tag="PKH58_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX89416.1"
                     /translation="MKKIFFLILAVFIVSCSGNRSDTKRTPVLEVEGKFLYQDEIDQI
                     IPATASIIDSADIAERYIKKWVTDVLIYENAKRNINKLNDIEKLVDEYRKSLIIHEYE
                     QALIKQRLTDKVSEDELKKFYEVYKSRLISQENLIKGILLVVPKNAPQTNKVREWVRL
                     SNTKSLENIEKYSLKNAISYDFFMNDWTPFTEILKKAPFRIEDSGAFIRTTTFAETSD
                     STKSYFLRITSALPSGQVEPYELAKDKISYILQNKKKSDFIIQFENDIYKDAVEEGTI
                     NYFKK"
     gene            complement(4568..6508)
                     /locus_tag="PKH58_10055"
     CDS             complement(4568..6508)
                     /locus_tag="PKH58_10055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX89417.1"
                     /translation="MKKVFVSVFGLCLAAVSALAQSSDPTLMKINGKEIPISEFEYIY
                     NKNNSNNVLDKKSLEEYVDLFVNFKLKVEEAIAQGLDTTQSFRSELALYRNQLAEQYL
                     TDSSMSSSLLKNAYDRKKEEVEVSHILVKIADTGTAADTLAAYNKALGIYKRAQREDF
                     GKLARETSDDQYAEKTGGYVGWINALRTPYSFENAAYSTPVGSVSNPIRTFLGYHIIK
                     VTGKRASQGEVHVKHIFILDNDPNSKNKAELKARIDSLYQRVKAGDDFGELAKKHSQD
                     PGSARQNGDLPWFGSGQMIPEFEKAAFSLNNPGDYSEPVRSAVGWHIIQLLEKRPLAG
                     FDEMKADLENQMKQNERNKLINQSFTDKLKKEYNYQEYAPALAEIYQLSEKYAPSDSL
                     FAVESKKLQKPLFSFADKTFTQNDFISNMLPAAAGFRGVRSDFIRDQYNAFAGNQLST
                     YEQTQLDRKYPEFHYLMQEYHDGILLFDISNREVWDKASKDNEGLTKYFETNKSNYAW
                     QKPHYKGRIIYCKDIPTLKAARLIVKRANNDSIDKYLNKRLNDSIQYVKVEKGLWVEG
                     ENKVIDVNIFKKGTYEAEKEYPYYFIAGKMLKTMPEDFTDVRGIITADYQDYLEKEWI
                     NSLRQKYAFTVDEKVLKTVKKN"
     gene            complement(6662..>7957)
                     /gene="guaB"
                     /locus_tag="PKH58_10060"
     CDS             complement(6662..>7957)
                     /gene="guaB"
                     /locus_tag="PKH58_10060"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNX89418.1"
                     /translation="MAIAIAREGGIGVIHKNMPIAEQARQVSMVKRAENGMISNPVTI
                     QKGANVGDALALMAEYKIGGIPVVDDEGYLVGIVTNRDLRFQRNLNRLIDEVMTKENI
                     VTTTRTTNLEEAADILQNHKIEKLPVVDEDNKLIGLLTYKDITKAKDKPMACKDSRGR
                     LRVAAGVGVTYDTFERISALVEAGVDAIVIDTAHGHSKGVIETLRKAKAQFPTIDIIV
                     GNIATAEAALALAEAGADAVKVGIGPGSICTTRIIAGVGVPQLSAIYGVSKALKGTGV
                     PVIADGGIRYSGDIVKALAAGANTIMAGSLFAGVEESPGETIIFNGRKFKSYRGMGSL
                     EAMEKGSKDRYFQDMEDDIKKLVPEGISARVPFKGSLFEVVYQMIGGLRAGMGYCGSH
                     NITELHKAKFTRITSAGVAENHPHDVTITSEAPNYSRGE"
BASE COUNT         1918 a         1693 c         1744 g         2602 t
ORIGIN      
        1 aatcaaccac cctgtcgttt accgctgcca gcaataattt tgagttggct attgcggtag
       61 ccattgccgt attcggaata aactccggcg aagcatttgc cgctgttatt ggtccgctgg
      121 tggaagtgcc agttatgatt ggcttggtga atgtggcatt ctggtttaag aacaaatggt
      181 tttaatataa acaaagaacc cgccaattcg gcgggttctt tgtttatatt ttactcactt
      241 actgaatttt catattgtta tttctcgttc tttgtcggtt gttggtattt tgacttccgg
      301 tattacggtt cattcctgtg tttctgtttg ctgcattgac attattcttc tgagcttgat
      361 ttgaggattg tttatcttta ctgtcatcct tttcttcagg gaatcccggc ttcttattac
      421 tttcgggtcg tttaggaggt tctgttctga caaatttcag gaatacatct tcgctttttg
      481 ctggcctttc cttttcgtac cagtaaaaat tgcgcagata aaggtcattt tcttccaggt
      541 taataatcgg gtacatagaa ccgctggaag ctgtggtaag tataatccgt tcaatcttct
      601 tatctttgaa aagcatcttt acatagctgc taaccgtttt attcagtccg attatatctt
      661 ttgtcttttc atcgcgcggg aaatagatgc tttcagcatt cccgtcaaca tccacccgtc
      721 tgatttgtcc gctgtcgagg taggctgtaa tctccttgcc tgataactga ttgtaataag
      781 tgagtgtgtc tttctgtact gccatcgccg actgcatgat atgaactttt tccacctgct
      841 gatttttggt ataagccttg atggaatcgc ccgaaagctg attgctgccc gaccagagca
      901 ccggcagaac tttgagattc atgatggagt cacgcgaggt gtaatagagc gaatcacaca
      961 taccctgtat atcggctctg tagaaccgta cattcttgtg tgctttgata gcgtcgtaag
     1021 tggaatcctt gaagttgaag agtgtatcgg cgctcataaa gagcgagtct tttcccgacc
     1081 agtccacaaa gagtgccgaa tcggtagcca gcccacattc ttttccttcg tcgtacgata
     1141 caaaatttcc gtaaagggtg gttttttgtt tcggatcatt gagttcgacg ttggaataag
     1201 cttcgctgta tttgtttttc ttgtcgtaaa agatcgtatc gccaaccagt gttttgccat
     1261 cattctgttc tacgaatgag cggttgagca gcatcatgcg gtctttggca gtatcatacc
     1321 agccgagttt gctgtagata ttggtctttt ccttgtagag tacatgcgag tcgccaacaa
     1381 tgtcggcaac ggaagttttt gtgttgtact tgagtgtatc ggaagtgatt gtgtaatttg
     1441 gattggtcat ctttacatct tccttgaaca gtgcctgacg ggtagcgggg gagtattgtc
     1501 cccataccga ggtaagtaca ttcacatcgt ccaccacttt accgccggtg taatagtaag
     1561 ccaggttggc aactcggtcg tagttcatac tgtcggttgt gagtgtggtg ttgcggttta
     1621 taagtttcac attgtcacgc agacgtgcaa gtcgggtatt tccgtcgtag ttgagatagt
     1681 cgccgtagat gaacagggta tcgccctgaa ccatctttac tcttccgaaa gcatcgaacg
     1741 tgttgcttat gtcgttgaaa taggcgctgt cgcagtacag aacggcattt tcatggcgga
     1801 agcgtacatt tcccctcagt acctgaatat cggggaaaag catctgatca taaaagagag
     1861 tttcgctgtt ttccagataa ataagagagg acttcggata tttggccgga ttagcgggtt
     1921 tgaatacagt ggcaccaaca ggtggtggct ggtaagcctg agtttttcct ccctttcggt
     1981 atttggatgc cgcagtttta gtctgctgat ttttctgata ctgaagtacc tcgttccgaa
     2041 gctgacgcgg agagtaagcc tgtgcacgaa gttcgggtaa aaaagtcagg gcaatgatta
     2101 ttaatatcat aaccctgaat atattggtat ttcgcaattt gtttgtcatc aaagaaatac
     2161 gataaatata ctattcttta atccagcctt tgtactggaa attgcagttg cgccattcgg
     2221 gcttaatgct gatataggtt tctttttgtt tcttcgaaat ccaattgtgg agaaattcct
     2281 gtttcttgtt gccttccatg atgtttttca gtacctgata atcatccatg atgttggctt
     2341 tatgggtcgg agttttgctt ttcagtttta cgatggtaat catttccttt ccggtaagcg
     2401 gatcaatcat cgtaaatgct ttggaaacct cgccgacatt catgttgtat gccgctttgg
     2461 ctacttccga tggcaaatcc tggtattcaa actttgaatt accggtattc tggttaacca
     2521 ttagtccggc attcatggcc gtatttctgt cctgcgaata atgcattacg gcctgctcga
     2581 aggtaatttt gttctggcgg ataaggtcag ccagtgagtc gagccgggtt atcccgtttt
     2641 gtttatcttc gatcgaggcg cgtggtttga gcagtatgtg acgacaattt actttatcgc
     2701 ctcttttgtc gatcagctga atgatgtgga agccgaactg tgattccacg atgcgtgaaa
     2761 ccttttttgg atcctgcagg ttgaaagcaa cagcggcaaa ttcagggtca agggtggcac
     2821 gcgcggtata accaagttca cctcccttaa cagcgcttcc ggggtcttcg gagtaaagtc
     2881 gtgccagcag gctgaaatcg gctggattgg ctgtagcgcg ttcggtgaac tcgcgcagtt
     2941 tttcttttac acggttcact tcagaaagtg gtataggcgg attaacagaa aggacctgca
     3001 gttcaacctg ctcgggtatt gtgggaagac tgtcctttgg tatgttgctg aaataacggc
     3061 gtacttcggc gggagttgcc tggatgccgc ttaccagctg actttgcatt tgctctatca
     3121 atgcttggct tttatatttc tcgcgaatga tttcgcgcaa ttgtgcaacg gttttatgtg
     3181 agtattcctc ggctttctcc accgaaccca catcagcaat gtatttgttc agatcggctt
     3241 cagcaaggga ggcaatataa ccttcgttga ctgtaacact gtcgagtgca gcctgatgca
     3301 ggaagagttt ctgtatggcc atttgctcgg gaatgacaca gtatgcatca ccctgaatgg
     3361 gctgtctgcg catttgtgca tccagaataa gagcctcgac gtcggatttg agaatggctt
     3421 catcccctac aatccagatg acttcatcta tggtattttt cttttgagct tgcaaacccg
     3481 atgcgagtaa gccgaaagcc agtaaaagca aagatatttt acgcattttt tacatgtaac
     3541 cgcgaacgga taaaaggttc acggattttt attgttatga ttattatttt ttcagaaccg
     3601 ggaaaaaata ccggaatgaa tatctgttga tttctatttc ttaaagtaat tgatagtacc
     3661 ttcctcaacc gcatctttgt aaatgtcatt ttcaaactga atgataaaat cggatttctt
     3721 tttgttctgc aggatatacg atattttatc tttagccagt tcatacggtt caacctgtcc
     3781 cgacggcaac gcgcttgtaa tccgcagaaa atacgatttt gtgctgtcag aagtctcggc
     3841 aaatgtcgtt gttctgataa acgcgcccga atcttctatt ctgaaaggtg cttttttcag
     3901 aatttcagta aatggtgtcc agtcattcat gaaaaaatca taactgatag cattcttcag
     3961 gctgtacttt tcaatgtttt cgagcgactt tgtattggag agccggaccc attcgcgtac
     4021 cttatttgtt tgtggcgcgt ttttgggtac aaccagcaat atgcctttta tcagattttc
     4081 ctgagatatc agtctggact tataaacttc gtagaatttt ttcagttcat cttcagatac
     4141 tttgtcggta agccgttgct tgatcaaagc ctgttcgtat tcgtgaatga tcagcgattt
     4201 gcggtattca tccaccagtt tttcaatatc gttcagttta ttgatattgc gtttggcatt
     4261 ctcatagata agtacatcgg ttacccattt cttaatatac ctttcagcta tatctgcact
     4321 gtcaatgatt gaagctgtgg ccggtatgat ttggtcgatt tcgtcctgat aaagaaattt
     4381 tccctccact tccagtaccg gcgttcgttt agtgtctgaa cggtttcccg aacatgatac
     4441 gataaaaacg gccagtataa ggaaaaatat ttttttcatt ttgtcaaaaa accagaactt
     4501 tcagaataca acaatgccaa aataaacgct caaagttaga acatttattt tggcattcgt
     4561 taaaatatta gtttttctta accgttttca gtactttttc gtcaacagtg aaggcgtatt
     4621 tctgtcggag cgaattaatc cactcttttt cgagataatc ctgataatcg gctgtgataa
     4681 ttccccgaac atcggtgaag tcttccggca tggtcttgag cattttacca gctatgaagt
     4741 agtatggata ttccttctca gcttcataag tgcctttttt gaaaatattc acgtcaatta
     4801 ccttgttttc accttcaacc cacaaacctt tttcaacctt tacatactgg atgctgtcgt
     4861 taagtctttt attcaggtat ttgtcaatcg aatcgttgtt agcacgtttc actatcagcc
     4921 tggcggcttt caaagtaggt atgtccttgc agtatatgat gcgacctttg tagtgtggtt
     4981 tttgccaggc gtaattcgat ttattcgttt caaagtattt ggtaagtcct tcattgtctt
     5041 tcgatgcctt atcccatact tcgcggttgc tgatatcaaa aagcagaatt ccgtcgtgat
     5101 attcctgcat caggtagtgg aattcgggat attttctgtc gagttgtgtt tgttcataag
     5161 ttgacagctg gttaccggca aaggcattat actgatccct gatgaaatca ctccgcactc
     5221 ctctgaaacc tgctgcggca gggagcatat tgctgataaa gtcattttga gtaaatgttt
     5281 tgtctgcaaa gctgaagagt ggtttttgca gtttcttgga ttcaactgcg aacaatgagt
     5341 cggaaggagc atatttttca ctcaactgat aaatttcagc taaagcagga gcatattcct
     5401 ggtagttata ttcttttttt aatttatcgg taaatgactg attaatcagt ttattgcgtt
     5461 cgttttgttt catctggttt tccagatcgg ctttcatttc atcaaaaccg gcaagaggcc
     5521 ttttctcaag caactgtatg atatgccatc ctacggcaga acgtacaggt tcgctataat
     5581 cgccgggatt attcaacgaa aaagcagctt tttcaaattc agggatcatc tgaccacttc
     5641 caaaccaggg aaggtcgcca ttctgacggg cagaaccagg atcctgagag tgtttttttg
     5701 caagttctcc gaaatcgtca cctgctttta cccgttgata cagtgaatca attctggctt
     5761 tcagttcagc tttgtttttt gaattaggat cattgtcaag tataaagatg tgttttacat
     5821 gcacctcacc ctgtgatgct cttttgccgg ttactttaat gatgtggtag cccagaaaag
     5881 tacgtatcgg atttgaaaca gaaccaacag gtgttgaata ggctgcgttt tcaaaagaat
     5941 aaggtgtgcg tagcgcatta atccatccga cataaccgcc ggttttctca gcatactggt
     6001 cgtctgaagt ctctctggcc agtttcccga aatcttcacg ctgcgcacgt ttatagattc
     6061 cgagcgcttt gttgtaggca gccagcgtat cggccgcagt gcctgtgtct gcaattttca
     6121 ccaatatatg gcttacttcc acttcttctt ttttgcggtc gtaagcattt ttcagcaaag
     6181 acgaactcat gctgctgtcg gtcagatatt gttctgcgag ttgattgcgg tacagagcta
     6241 attcagaacg gaacgattgt gtggtatcca aaccctgcgc tatggcttct tccactttca
     6301 gtttgaagtt gacaaataaa tccacatatt cttcaaggga tttcttgtcc agcacattat
     6361 tcgaattgtt tttattgtaa atgtattcaa attccgaaat aggaatttct ttcccgttaa
     6421 ttttcatcag agtagggtca gaagattgag ccaaagctga tactgcggct aaacatagtc
     6481 caaaaacaga aacaaaaact ttcttcatac agattaaaaa tttgaattat tttttttatg
     6541 ctgattaaaa acgctgattt cctgataaaa ttgtgacgca aaagtgtttt cacaaagtta
     6601 ctccctgttg aagagagtaa ttttgtgatt aacagtaatt tgttttcgta tttcaatgaa
     6661 tttattcacc ccggctgtag tttggagctt cactggttat ggtcacatcg tgagggtgat
     6721 tttcagctac acctgcactt gtgatacggg taaatttagc tttgtgaagc tcggtaatgt
     6781 tgtgagagcc gcagtatccc attccggcac gaagtccgcc gatcatttga tatacaactt
     6841 cgaaaagtga gcctttgaat ggcacgcggg ccgaaattcc ttcgggaacc agtttcttga
     6901 tatcatcttc catatcctgg aaataacggt ctttcgaacc tttttccatc gcttcaagcg
     6961 aacccatacc ccggtacgat ttgaatttac gtccgttgaa gatgatggtt tcacccggag
     7021 attcttctac accggcgaat agcgatccgg ccatgattgt attggctccg gctgccaggg
     7081 cttttacaat gtcgcccgag taacggatac caccgtctgc aataactgga acgccggttc
     7141 ctttcaatgc tttggaaacg ccgtatattg ccgaaagctg gggaacacca acccctgcga
     7201 tgatgcgggt ggtacaaata gaacccggac caatacccac tttaaccgcg tcggcacctg
     7261 cttccgccag ggctaatgca gcctcggctg tggctatgtt gccaacaatt atatcaatag
     7321 taggaaattg tgcttttgct ttacgcaggg tttcgattac cccttttgaa tgtccgtgag
     7381 cggtatcaat tacaatagca tccacaccgg cttccaccag cgcgcttatc cgctcgaatg
     7441 tgtcataagt aacgccaaca ccggcagcta cgcgcaatcg tccccgactg tccttgcagg
     7501 ccataggttt gtctttggct ttggtaatat ccttgtaggt gagcagcccg atcagtttat
     7561 tgtcctcatc cacaaccggc aatttctcga ttttatggtt ttggagaata tcggcagctt
     7621 cttcaaggtt ggtggtacgg gttgttgtaa cgatattttc cttggtcatc acttcgtcga
     7681 tcagtctgtt caggttacgc tgaaagcgca aatcgcggtt ggtaactatt cccaccagat
     7741 atccttcgtc atccacaacg ggtatacctc cgattttata ttcagccatc agagcgaggg
     7801 catcgcctac gtttgctcct ttctgaatag ttactggatt ggaaatcatt ccgttttcgg
     7861 cgcgtttcac catgcttacc tgtcttgcct gttcggcaat aggcatgttt ttgtggatca
     7921 caccaattcc accttcacgg gcaatggcaa tagccat
//
LOCUS       DAOPYB010000142         7870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_11081_length_7870_cov_8.195393, whole genome shotgun sequence.
ACCESSION   DAOPYB010000142 DAOPYB010000000
VERSION     DAOPYB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11081_length_7870_cov_8.195393"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..936
                     /locus_tag="PKH58_10065"
     CDS             160..936
                     /locus_tag="PKH58_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNX89419.1"
                     /translation="MKKFILDLKITQKVQLNKQNVLLKLTSKDLLPEILPGQFAEIRV
                     DGSPATFLRRPISINFIDSARNELWLLVQIVGEGTKHMAEMNPGDLLNAILPLGNTFT
                     IPSDKNAKIVLIGGGVGVAPLYMLGEKLKKLGYKCIFLLGARSRSDLLLLNEFEKIGQ
                     VHTTTEDGSHGEKGYVTQHSLLFNSDFDYIYTCGPTPMMKAVASYAAKNNIFCEVSLE
                     NMMGCGIGACLCCVTDTTEGNLCVCTEGPVFNITKLKWQI"
     gene            924..1835
                     /locus_tag="PKH58_10070"
     CDS             924..1835
                     /locus_tag="PKH58_10070"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNX89420.1"
                     /translation="MANLITKLGRLQMKNPVMTASGTFGYGTEYSDFMDLSRIGCIIV
                     KGTTLRERQGNQYPRMAETASGMLNAVGLQNKGVNYFIEHIYPTIKDLDTNVIVNVSG
                     SEIDDYVKTAEIINELDKIPGIELNISCPNVKEGGMAFGVSCAAAAKVVSAVRKVYDK
                     ELMVKLSPNVTDIAEIAKAVEDTGADSISLINTLLGMAVNAKTRKPILSTITGGLSGP
                     AVKPIALRMVWQVAHAVKVPVVGLGGIMNATDAIEFMLAGATAIQVGTANFINPAISE
                     KIVEGINDYLDTNNFSSVAEIIGALEI"
     gene            2094..3551
                     /locus_tag="PKH58_10075"
     CDS             2094..3551
                     /locus_tag="PKH58_10075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNX89421.1"
                     /translation="MAKVLILGAGIAGHTAALIASRKLGKSHEVVVVSPNSNYQWVPS
                     NIWVGIGLMTVKQVIFPLAPVYKRMGIVFKQAKAISIHPEGDDVSSKQYVQIQYVTSD
                     KKDQTEKVEYDYLINATGPKLNFEATEGLGPDKNSFSVCTYDHAAHTWEQLQKCFSKM
                     EKGEKQHFVIGTGNGMATCQGAAFEYALNIAFEIKRRKLLDFSEITWISNEYELGDFG
                     MGGAYVKQGGYVTPTKIFAESIFKEYGINWIVGAAVQKVEPGVLSYENLAGEYHKLEF
                     DFSMLIPPFAGVGLKAYNRKMEDITSTMFAPNGLMKVDADYTPKPFEEWKASDWPKTY
                     ISPVYDNIFAAGIAFAPPHTISKPMTSKNGTPITATAPRTGMPSGVMGRIVALNIVEQ
                     IKSGSKTLRHEASMAKMGAACIVSAGYGIRRGKAAVMTVFPIVQDWEKYPKWGRNIGY
                     TVGEIGLAGHWMKLFMHYMFIYKAKAMPLWWMIPE"
     gene            3592..3753
                     /locus_tag="PKH58_10080"
     CDS             3592..3753
                     /locus_tag="PKH58_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89422.1"
                     /translation="MANLEKTTPYSESSFATEPTKWTKFWRVSILYQLIRFFVLNLKI
                     MRIVVGGHS"
     gene            3777..4520
                     /locus_tag="PKH58_10085"
     CDS             3777..4520
                     /locus_tag="PKH58_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNX89423.1"
                     /translation="MAFIYLYIRYMSVLKCRDMEVNEIIAELHKLASEDHYNKLSRFG
                     IPADKAIGVKIPDLRRLAKKIGKNQKTGFSLWKTDIHEARLLASMIMDGRLLTEYEFD
                     GLVNDFDSWDICDCTCSMLQKSLFARNKIDEYADNQNEYVKRTAFVLICYFAVIDKSK
                     SDDFFNPFFKIIEKEAWDDRNFVRKAVNWALRQTGKRNENLRIKAIETANDILKQNTK
                     SARWIAKDAIRELNDEKIIVRIRKKADLL"
     gene            complement(4515..4982)
                     /locus_tag="PKH58_10090"
     CDS             complement(4515..4982)
                     /locus_tag="PKH58_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNX89424.1"
                     /translation="MLQLNLPEYQFRLKQTEKGLLILDSLRKRFVKLTPEEWVRQNFL
                     RYLIEHKQYPLALMAVEKQLIVNGLKKRCDAILYDIKGNPMIIIELKAPNIPITQLVF
                     DQVAVYNSKLKVKYFMISNGFEHYCCKVNPENSSYLFFEGIPDYSLLLSDNSL"
     gene            5080..5853
                     /locus_tag="PKH58_10095"
     CDS             5080..5853
                     /locus_tag="PKH58_10095"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNX89425.1"
                     /translation="MKTKEEIVQNWLPRYTKRKLEDFTEYILLTNFSNYVEMFASWFD
                     VPVFGRDGNMINASANGITIINFGMGSPNAAMIMDILSAIHPRAVLFLGKCGGLRDKN
                     QIGDYILPSAAIRGEGTSNDYFPPEIPALPAFNLQRAVSSNIRDFGKDYWTGTVYTTN
                     RRVWEHDSKFKEYLRTTRAMAIDMETATLFTAGFYNGIPTGALLLVSDMPLHEDGIKT
                     EESDKKVTANFVEEHLRIGVKSLSSLLNNSRTIKHLRFE"
     gene            5887..6891
                     /gene="holA"
                     /locus_tag="PKH58_10100"
     CDS             5887..6891
                     /gene="holA"
                     /locus_tag="PKH58_10100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNX89426.1"
                     /translation="MQTYQQIMTDLRRKAYSPVYFLMGEEPFYIDLISDFMEDKILDE
                     SEKDFNLTVLYGKDVDIATVINAAKRYPMMSEYQVVIVKEAQLIKDWEVFSYYLANPL
                     KSTILCICYKYGKPDGRKKWVQDLNKTAVVLESKKLYDNQVGLWIREYLADRKVRISE
                     KAEMMLTEFLGSDLSKIANELDKLLITRKPEENHITPEMIEKNIGISKDFNVFELQAA
                     LINRDVLKANRIIRYFGDNKKANPIQMVLSQLFSFFSNLMMFHYLPDKSPATVAAELK
                     ITAYFTKDYVHAAKTYNAWKTMNIISWIRETDARSKGIEDTGSESVDLMKELIFKILH
                     "
     gene            6891..7835
                     /locus_tag="PKH58_10105"
     CDS             6891..7835
                     /locus_tag="PKH58_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNX89427.1"
                     /translation="MNKFFEYFKKYNSYYYLLLVFFCLTPWATPPVALFLGLIFTLTL
                     GSPFPVFNKKASKYLLQIAVIGLGFGMNLQESLKAGTDGILFTIISVVSVIVIGILAG
                     KLFKVDKVTSYLISSGTAICGGSAIAAVGPIVRADESQMSVSLATVFVLNAIALFIFP
                     VIGHWLDLSQQQFGTWAAIAIHDTSSVVGAGSAYGEEALKVATTVKLTRALWIIPLSI
                     FTTFYFKSKGDKMYIPWFILFFIVAMLLNTYFNIDHKVTESIVKVSRQCLTLTLFFIG
                     AGLSRENLKTVGVRSLLLGVTLWFFIAVISIVFIIFYF"
BASE COUNT         2555 a         1300 c         1586 g         2429 t
ORIGIN      
        1 agcctgtccg gcttgaggac aaaacaagat aaatttttta tcggacatca ataatcctga
       61 ataaagtttg aagaagtcgg gaacttatcc actttgaaaa ttgagttgaa ttttgtattt
      121 ttgcacttcc aagtttggat aacaactaac cgaaatccga tgaagaaatt cattcttgat
      181 cttaaaataa cgcaaaaagt acaactgaat aagcaaaatg tacttctaaa attaacttcc
      241 aaagacttac ttccggaaat attacccgga caatttgctg aaatccgtgt tgatggctca
      301 ccagcaacat ttttacgacg tcccatatcc attaatttta ttgattctgc ccggaatgag
      361 ctgtggctgc tggtgcagat tgtaggcgaa ggtacgaaac acatggcaga gatgaaccca
      421 ggcgatttac ttaatgcaat attaccgctg ggcaacacct tcactatacc ctccgacaaa
      481 aatgcaaaaa ttgttcttat tggaggtggt gttggagtag ctccactcta catgctgggc
      541 gaaaaactta aaaaattggg ttacaaatgt atttttttac ttggagcccg ttcccggagc
      601 gatttacttt tacttaatga atttgaaaag atcggacagg ttcataccac tacagaagat
      661 ggttctcatg gagaaaaagg atatgtaacc cagcattcgc tcttatttaa ttctgatttt
      721 gattatatat acacctgtgg tccaactcca atgatgaaag cagtagcttc ttatgcggca
      781 aaaaataaca ttttctgtga agtatcgctc gaaaatatga tgggatgcgg tataggagct
      841 tgtctttgct gtgtaactga taccactgaa ggaaatttat gtgtttgtac tgaaggtcct
      901 gtatttaata taacaaagtt gaaatggcaa atttaataac gaaactcggc agattacaaa
      961 tgaaaaatcc cgtgatgaca gcttcgggga catttggtta tggtacagag tattccgact
     1021 tcatggattt atcgcgaata ggttgtatta ttgtaaaggg aacaacgctt cgcgaacgtc
     1081 agggaaatca atatccgcgt atggctgaaa cggcttcagg gatgttaaac gctgtaggac
     1141 tgcaaaataa gggtgtaaat tactttattg aacatattta tccgacgata aaagaccttg
     1201 atacaaatgt aatcgtcaat gtatccggat ctgagattga tgattatgta aaaacagcag
     1261 aaataatcaa cgaactggat aaaattccgg gaattgaact gaatatttcc tgtccgaatg
     1321 tgaaagaggg tggaatggca tttggtgtat catgtgcggc agcagcaaaa gtagtttcgg
     1381 cggtcagaaa agtgtacgat aaagagctga tggttaaact ttcgccaaat gtgacagata
     1441 ttgctgaaat tgctaaagcg gtggaagata cgggagcaga tagcatatca ttgattaata
     1501 cccttttggg aatggcagtc aatgcaaaaa cacgcaaacc catactttca acaataacag
     1561 gcggtttatc ggggcctgca gtgaaaccca ttgcattgcg tatggtgtgg caagtagcac
     1621 atgccgttaa agtacctgtt gttggacttg gaggcatcat gaacgctact gatgccattg
     1681 aatttatgct tgcaggtgcg acagcaatcc aagtaggtac tgcaaacttt attaatcctg
     1741 ctatttctga gaagatagtg gaaggaataa atgattattt agacacgaac aacttttcat
     1801 cagtggcaga aattatcggt gccctcgaaa tttgaacata catacaaatt ttatctgtat
     1861 ttctgattat ttatttaatt caacattttt ttctttccat aatattttta taaaataatt
     1921 atttttaata atttctccta taaaggtatc actattcaga attatttata tttttgtcat
     1981 agtctttatt ttttgattta catacttttg actatataaa ttcagaaaaa tgtaaaaaaa
     2041 attgaaattc attccaaaat cacatacaga gtttcattaa aaatcaatga tttatggcaa
     2101 aagttctaat tttgggtgcc ggaattgccg gccatacagc tgcattaatt gcaagcagga
     2161 aacttggcaa atcacatgaa gtggttgttg tcagtccaaa ctccaattat caatgggtgc
     2221 catccaatat ttgggttggt atcggtctca tgaccgttaa acaggttatt tttcctctgg
     2281 ctccggttta taaaagaatg ggtatagttt tcaaacaagc aaaagcaata tccatccatc
     2341 ctgaaggtga tgatgtctct tcaaaacagt atgtacaaat tcagtatgtt acctcagaca
     2401 aaaaagatca gacagaaaaa gtagaatatg attatctgat aaatgctaca ggtccaaaac
     2461 tcaattttga ggctactgaa ggccttggtc ccgataaaaa tagtttctct gtatgcacct
     2521 atgatcatgc tgcacatacc tgggaacaac tgcaaaaatg tttttcaaaa atggaaaagg
     2581 gggaaaagca acattttgtt attggtacag gtaacggaat ggcaacttgt cagggagctg
     2641 catttgaata cgcattaaat atagcctttg agattaaaag acgcaaacta ctggattttt
     2701 ccgaaataac gtggatatca aacgaatatg aacttggtga ttttggtatg ggaggagctt
     2761 atgtgaagca gggtggttac gtaacaccta caaaaatctt tgccgaatcc atcttcaaag
     2821 aatatggaat aaactggatt gtgggtgcag cagttcaaaa agtggaaccc ggtgtgttga
     2881 gttacgaaaa tcttgcaggt gagtatcata agttagaatt cgacttttcc atgttgatac
     2941 ctccatttgc cggagtcggg cttaaagctt acaacaggaa gatggaagat atcacatcca
     3001 ccatgtttgc ccctaatggt ttaatgaaag ttgatgcaga ctatactcca aagccttttg
     3061 aagaatggaa agcgagcgat tggccaaaaa cgtatataag tcctgtgtat gacaatatct
     3121 ttgccgctgg tatagctttt gctccacctc ataccatttc aaaaccaatg acctcaaaga
     3181 acggaactcc tattacagcg acagcacccc gtactggaat gccttcgggt gtaatgggta
     3241 ggattgtagc cctcaatatc gtagaacaga ttaaatcggg ttccaaaacg ctgagacatg
     3301 aagcttctat ggcaaaaatg ggtgccgcat gtattgtctc agccggatat ggtatcaggc
     3361 gtggcaaagc agcagtgatg actgtttttc ctattgtaca ggattgggaa aaatatccga
     3421 agtggggcag gaatatcggc tatacggttg gagaaattgg tctggccggt cactggatga
     3481 aactgtttat gcattatatg tttatttata aagctaaagc tatgcccctt tggtggatga
     3541 tacccgaata agattaaagc gagtaataac acattaatat attttaccat tatggcaaat
     3601 cttgaaaaga caactcctta cagcgaatct tcatttgcaa cggaacctac aaaatggaca
     3661 aaattctggc gtgtaagcat cttgtatcaa ctgatacgct tttttgtact caatctgaaa
     3721 ataatgcgta ttgttgttgg gggacattcc taataacaaa tcaaaaaatg ttatgtatgg
     3781 cattcatata tctgtatatc agatatatga gtgttcttaa atgtagagat atggaagtta
     3841 atgaaataat cgccgaactt cacaaattag ctagtgaaga tcattataat aaattatccc
     3901 gatttggcat accggcagac aaagccattg gtgtgaagat acccgatctg cgcagactgg
     3961 caaaaaagat tggaaaaaat caaaaaacag gattttcatt atggaaaaca gacattcatg
     4021 aagcacgttt actggcttcg atgataatgg atggccgttt gcttacagaa tatgaattcg
     4081 acggtctagt caatgatttt gactcctggg atatctgcga ttgcacttgc tctatgcttc
     4141 aaaaatcatt gttcgcaagg aataaaatag atgaatatgc tgataatcaa aatgaatacg
     4201 taaaaagaac agcttttgtc ctgatttgtt attttgcagt gatagataag tccaaatccg
     4261 atgatttttt caatcctttt ttcaaaatta ttgaaaaaga agcctgggat gaccggaatt
     4321 ttgtgagaaa agctgtgaac tgggctttac gtcagacagg taaaagaaat gaaaatttac
     4381 gtataaaagc aattgaaact gccaatgata tactaaagca aaatacaaaa tcggccagat
     4441 ggattgctaa ggatgctatc agagagctga atgatgaaaa aataattgtc agaatccgaa
     4501 agaaggctga tttgttataa agaattatca gatagaagca gtgagtaatc cggaatacct
     4561 tcaaaaaata aataggagga attttccgga tttactttac agcaatagtg ttcaaaaccg
     4621 ttgcttatca taaaatattt gactttcagt tttgaattgt acacagcaac ctgatcaaaa
     4681 accagttgtg taataggaat atttggtgct tttaattcta taataatcat tggattaccc
     4741 tttatatcat acaggattgc gtcgcatctt tttttcagtc cgttgacaat tagttgtttt
     4801 tctacagcca tcaatgcaag tggatactgc ttatgttcaa taagataacg caggaaattc
     4861 tggcgtaccc attcttctgg agtaagtttt acaaatcttt tcctcaaact atcgagaatt
     4921 aataagcctt tttctgtttg ttttaagcga aattgatatt cgggtaagtt gagttgcaac
     4981 atttttttag tatttttgtc ttcagaaata taaactacaa cgaaattaca aaaatattta
     5041 cttacaattt ttaaaaatca gaataataaa attgatttta tgaaaactaa agaggagatt
     5101 gtacaaaact ggcttcccag atataccaaa aggaaattgg aagatttcac agaatatatt
     5161 ttattaacaa atttttctaa ttacgtcgaa atgtttgcca gttggtttga tgtaccggtg
     5221 tttgggagag atggcaatat gataaatgca tcagcaaatg gaataacgat tattaatttt
     5281 ggcatgggta gtcccaatgc tgcaatgata atggatattt tatcagctat acatcctcgt
     5341 gcggtactgt ttctgggcaa atgcggtgga ttacgtgata aaaaccagat aggcgattat
     5401 atccttccaa gtgcagccat tcgtggagaa ggaacttcaa atgattattt tccaccagag
     5461 atacctgctt tgcctgcatt taatttacaa cgtgcggttt cctcaaatat tcgtgatttc
     5521 gggaaagact actggacagg aacagtttat acaacgaatc gcagggtatg ggaacatgat
     5581 agtaaattta aagaatatct tcgtacaacc agggcaatgg ctatcgatat ggaaacagcg
     5641 actttattta ccgccggatt ttataatgga attccaaccg gagctttgct gttggtttca
     5701 gatatgccac ttcacgaaga tggtattaag acagaagaaa gtgataaaaa agtaacagcc
     5761 aactttgtag aagaacatct tcgcattggt gtaaaatcac tttcatcatt acttaataat
     5821 agccgtacaa tcaagcattt acgatttgaa taattttgtt ttttcgttta acaaccagga
     5881 taatatatgc aaacctatca gcaaataatg acagatttac gccgtaaagc gtatagtccc
     5941 gtttattttc tgatgggaga ggaacctttt tatattgatc tgatttcaga ttttatggaa
     6001 gataaaattc tggatgagag tgaaaaggat tttaatttga ctgtacttta cggcaaagat
     6061 gtggacattg caacagtgat taacgctgct aaacgatatc cgatgatgtc tgaatatcag
     6121 gttgttattg tcaaggaagc tcaattgatc aaagactggg aagtttttag ttactatctt
     6181 gccaatccgt tgaaatcaac catcttgtgt atttgctaca aatatggcaa accagatggt
     6241 cgcaaaaaat gggttcagga tctgaataaa acagccgttg ttctggaaag taaaaaactt
     6301 tacgacaacc aggttgggtt gtggattaga gaatacctgg cagaccgtaa agtgaggatt
     6361 tcggagaaag ctgaaatgat gttgactgaa tttctgggtt cagatttgtc gaaaatagcc
     6421 aatgaactgg ataaactgct cattaccagg aagcctgagg aaaatcatat tacccccgaa
     6481 atgatagaaa aaaatatcgg gataagtaag gatttcaatg tctttgagtt gcaagcggcg
     6541 cttatcaacc gggatgtact taaagccaat cgaattatac gctattttgg ggataataag
     6601 aaagcaaatc ccatccaaat ggttctgagt caattgttca gttttttcag caatctgatg
     6661 atgtttcatt acctgcctga taaaagccct gccaccgttg cagctgaact gaagataaca
     6721 gcttatttca caaaagatta tgttcatgca gccaaaacat ataatgcatg gaaaactatg
     6781 aatataatca gctggatacg cgagacagat gctcgcagca aagggattga ggacaccgga
     6841 tcagaatccg ttgatttgat gaaagaatta atatttaaga ttttacattg atgaataagt
     6901 tttttgagta ttttaaaaaa tataatagtt attattatct gcttctcgta tttttttgtc
     6961 ttacaccctg ggctacacct cctgtagcct tatttttggg actcattttt acattaacgc
     7021 ttggttctcc ctttccggtt ttcaacaaaa aagcctcaaa atacttacta caaatagctg
     7081 ttatcggatt aggttttggt atgaacctgc aagaatcact caaagcggga actgatggaa
     7141 ttttattcac aattatttct gtagtgagtg taattgtaat tggaattcta gctggtaaac
     7201 tctttaaagt agacaaagtt acttcttatc ttatttccag tggaacagcg atttgtggtg
     7261 ggagtgcgat tgcggcggta ggtccaattg taagggctga tgagagtcaa atgtccgttt
     7321 ctctcgcaac tgtttttgta ttgaatgcga tcgcgttgtt tattttccct gtaataggcc
     7381 attggcttga tttaagccaa caacagtttg gaacgtgggc agctattgcc attcatgata
     7441 caagttcagt ggtcggagcc ggttctgcat acggtgagga agcgcttaaa gtggctacaa
     7501 cagttaaatt aacgagagca ctatggatta ttccactttc catatttact actttttact
     7561 ttaaatcaaa gggagacaag atgtatattc cctggtttat attatttttc atagttgcca
     7621 tgttgttaaa tacttatttc aatattgatc ataaagttac agaatcgatt gtaaaagttt
     7681 cacgccagtg tttgacactc actttattct ttattggggc gggattatct cgcgagaatt
     7741 tgaaaacagt aggtgtaaga tctttgttat taggtgtcac attgtggttt ttcatagcag
     7801 taattagcat agtttttatc attttttatt tctgatgaaa aacgttattt ttatatttat
     7861 aattataatt
//
LOCUS       DAOPYB010000143         7823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_11175_length_7823_cov_9.673532, whole genome shotgun sequence.
ACCESSION   DAOPYB010000143 DAOPYB010000000
VERSION     DAOPYB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11175_length_7823_cov_9.673532"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..831)
                     /locus_tag="PKH58_10110"
     CDS             complement(133..831)
                     /locus_tag="PKH58_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HNX89428.1"
                     /translation="MNKQYRHIFFDLDHTLWDFERNADETKRELYQEFKLGERGIPSY
                     EAFREKYVVINAGLWALYREDKIEKEELNFKRFFLTLCEFGINDMSLGASLAAEFIKG
                     ISTKTYLFPHATELLEYLFPKYPLYIITNGFEEVQFSKLKNSRLERFFTSVITSEEAG
                     VKKPERAIFEYALRKTGASASESLMIGDDLEVDMRGAREAGIDQLLVNHEAFQHQEPV
                     TFEVFSLKEIKLIL"
     gene            942..1811
                     /locus_tag="PKH58_10115"
     CDS             942..1811
                     /locus_tag="PKH58_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNX89429.1"
                     /translation="MEKDRISTPSGGYAGVAVSLLLFAGAIVLLTMFNHTLAGILGGI
                     ICLVLSCFIVPGFLVLNPNEASVLILFGAYKGTIKSNGFYWINPFFVKKRVSLRARNL
                     TSDALKVNDKLGNPIMIGIVLVWKVEDTYKAAFAVDDYANFVQIQSESAVRKLAGHYA
                     YDNFDDEQTEITLRSGGEEVNHVLEKELSERLQIAGIKVIEARIAHLAYSPEIAGAML
                     RRQQATAVVAARTKIVEGAVSMVKLALNKLAENQIVELDEERKATMVSNLMVVLCSDK
                     DATPVVNAGTLYQ"
     gene            1815..2366
                     /locus_tag="PKH58_10120"
     CDS             1815..2366
                     /locus_tag="PKH58_10120"
                     /inference="COORDINATES: protein motif:HMM:NF039358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6141 family protein"
                     /protein_id="HNX89430.1"
                     /translation="MGVLLFREEQPVGQTMIPLVQVLVWLFVTGLFGWGFYEQLVLGK
                     PWGDNPMSDTALIVIGITVIIFMGLMTVLSIRNSLITEVHSDGFYYRFPPFINRMRCI
                     PVEQIASVEVRKYGLFPGSFGWGIKRRPLRRTTIYQAGGNKVVVITLKTGRRIIFSTK
                     KMEEMRRAVDRMMAERDNHKTIF"
     gene            2410..2589
                     /locus_tag="PKH58_10125"
     CDS             2410..2589
                     /locus_tag="PKH58_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNX89431.1"
                     /translation="MSKKKVFALRIDEQLLAAVERWAADDFRSTNGQLEWIIHNALKE
                     AGRQPKQTAEDGKQK"
     gene            complement(2682..3104)
                     /locus_tag="PKH58_10130"
     CDS             complement(2682..3104)
                     /locus_tag="PKH58_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNX89432.1"
                     /translation="MKKIILLLAVIAACGWANAQPSVHATANEKAISNPNGPKARFDK
                     MINDFGEIDRGIPKTAEFTITNDGKEPLIIQKAQASCGCTNLKYSQEPILSGKSAVIS
                     VTFNAASVGPFMKTITVTTNAEASPSVLQIKGTVVKKE"
     gene            3279..4556
                     /locus_tag="PKH58_10135"
     CDS             3279..4556
                     /locus_tag="PKH58_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX89433.1"
                     /translation="MRIKHIRFVVLLGSFAIIGIFAIQWYFLKKEWNNREKQFTQTVM
                     IGLRNVADVLHKINQTNPTTTNPVRQLSSNYFVVDVNGVIDANVLEHYLKLEFERLNI
                     RTDYEYAIYNCYTGVMEYGNYFFYNGKIKHNAISVNLPKYDEYNYYFGVNFPLLKNTI
                     AGGMTMWFFLMGILFVAVIFFGYSIFVILQQKRLSEMQRDFINNMTHEFKTPISSINI
                     SADVILNPESLMEPVRLQTYASVIKQETSRLNDQVDKVLQIARIEKQGFHLRKEVFDL
                     HVLIRNVAENYRKDSGRNINIDTNLAPGTVEVNADKLHVANIFHNLLDNAMKYSGESP
                     EVTISSSLRGGRVQVRLRDNGPGIGRQHQKKIFRKFYRIPTDNVHDVKGFGLGLFYVK
                     NICDAHRWRISLNPDERNGAEFILEIPVLKSAL"
     gene            4553..5257
                     /locus_tag="PKH58_10140"
     CDS             4553..5257
                     /locus_tag="PKH58_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX89434.1"
                     /translation="MKNARILYVEDDVTLSFVTRDNLERSGYVVDSCEDGLQASEKFV
                     PGKYDLLILDVMLPKMDGYTLAKKIREKDESVPILFLSAKSTKDDKILGLQLGADDYI
                     TKPFSIEELILKIEVFLKRRSIDHGNTMQADSFRIGRFMFSSSGRQLILDDLKQTLTY
                     RESELITYLFRNLNKVVKREEILMAVWGNDNYLISRSLDVFISRLRKLFRGDPSIKIE
                     NIHNIGYRMSITESRE"
     gene            5523..5921
                     /locus_tag="PKH58_10145"
     CDS             5523..5921
                     /locus_tag="PKH58_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89435.1"
                     /translation="MRHMLISIICLMLISSTCKKEEDCHSYIDFTNQSEKDIIFGLRA
                     TDFKGNCRLDGNKVMKNETFDFRPYNSCIEDNLNSNTPLDIYIVDSDKYNEPSVFYDC
                     DSIEIKNKILKHYKLTLEDLKKLDFTIIYP"
     gene            5918..6211
                     /locus_tag="PKH58_10150"
     CDS             5918..6211
                     /locus_tag="PKH58_10150"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX89436.1"
                     /translation="MKLIKGIILCIVLSGCGKTPIADFSWSPQNPKAGEEVQFTNLST
                     DAKEFSWNFGDMDISNESNPKHTYQKSGNYIIDLNATSGLKTDIKTVTIIVTP"
     gene            6842..7612
                     /locus_tag="PKH58_10155"
     CDS             6842..7612
                     /locus_tag="PKH58_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89437.1"
                     /translation="MVFFFLQSNKLFNKTKCFLSLLLILLSYGDLKSQNQKVFANTDL
                     DTIIMYESTAYLNLGGWVGGAKIFKKNNELVLHTTAYSQHLESFSKIIYRKCDTCKKN
                     EFIVRIFNLNEPVNNQCMIFGVVNYIDFGIVEFLNKNFVVFNLTEMPPDSMLRISFCL
                     EYYPLMLKLTNISGGIYDIHLSKSYKPLSLFENGLIIRCKYLNSTCLECSYKMENPDK
                     QINLVIKRKNKSPIVLLTINRKSTYRFSLSIPNVNSMP"
     gene            complement(7609..>7823)
                     /locus_tag="PKH58_10160"
     CDS             complement(7609..>7823)
                     /locus_tag="PKH58_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89438.1"
                     /translation="AEKDAVMPQVYSARVRRRAQAKGEYQSPRERISACGREKSSRGS
                     GESYGSSLTVSALIIKTLTYYQRPCF"
BASE COUNT         2280 a         1567 c         1763 g         2213 t
ORIGIN      
        1 tcagaggcag cttttttttg ctggaaaagc ctgcgataca ctgctttttt cagtataccc
       61 taattaattc ttacaaccgg gaacccggga aacggcatgg ctcaagatga actgcaaatt
      121 cccggttcag atttacagga tcaatttgat ctctttaaga gaaaagactt caaaggtcac
      181 cggttcctgg tgctgaaatg cttcgtggtt aaccagcaac tggtcgattc cggcttcacg
      241 ggcgccccgc atatcaactt caaggtcatc gccgatcatc aggctttcgg aggctgacgc
      301 ccctgttttt ctgagcgcat attcaaaaat tgcacgctcc ggttttttta caccggcttc
      361 ttcactggtt attacgctcg taaaaaaacg ttccaaccgt gaattcttca gcttgctgaa
      421 ctggacttct tcaaatccgt tggtgatgat atataaggga tatttcggga aaaggtattc
      481 caggagctca gttgcatggg ggaaaaggta cgttttggtt gagatgcctt tgataaattc
      541 cgctgctaaa gaagccccca gcgacatgtc gttaattcca aactcgcaca gggtaagaaa
      601 aaaccgcttg aaattcagtt cttccttctc tattttgtct tcccggtaaa gcgcccataa
      661 tccggcattg atgactacgt atttttcgcg aaaagcttca taagaaggga taccccgttc
      721 ccccagctta aactcctgat aaagttcccg cttggtttcg tcggcattgc gctcaaaatc
      781 ccataaagtg tggtcgagat cgaaaaaaat gtgcctgtat tgtttattca tgaagcgcaa
      841 aattatcctt ttaccgcctg ctccgaaaat aattttcacc ttttgtcgta acgttcctaa
      901 aatgtcgtaa tattgtgata tcaaaataat atcaaaatac tatggaaaaa gatcgtattt
      961 cgaccccatc cggcggttat gccggagtag cagtatccct gcttttattt gcaggcgcga
     1021 tcgtattgct tacaatgttt aaccatacac tggccggcat acttggcggg atcatctgcc
     1081 ttgtattgtc gtgttttatc gtaccgggat tcctggtgct gaacccaaac gaagcgtcgg
     1141 tattgattct cttcggcgcc tacaagggaa cgatcaagtc gaatggcttt tattggatta
     1201 atccgttctt tgtcaagaaa agggtatcgc tccgggcgag gaatctcacc agtgacgcct
     1261 tgaaggtgaa tgataagtta ggcaatccga ttatgatcgg aattgtgctg gtgtggaagg
     1321 tagaagatac ctacaaggca gcttttgcag tggatgatta tgccaatttt gtccagatcc
     1381 agagcgaatc agccgttcgc aaacttgccg gccattatgc ctatgataat ttcgatgatg
     1441 aacagacgga aattaccctg cgctcgggag gtgaagaagt aaaccacgtg ctggaaaaag
     1501 aactgtcgga acggctccag atagccggaa taaaagtcat agaggcgcgt attgcccacc
     1561 tggcctattc gcccgaaatt gccggcgcta tgctgcgccg tcagcaggct acggctgtcg
     1621 tggctgcgcg taccaaaatc gttgaagggg ccgttagtat ggtgaaactg gctcttaata
     1681 agcttgccga aaatcagatt gttgaacttg atgaagagcg aaaagcgacg atggtaagta
     1741 acctgatggt ggtactatgc tcagataagg atgcaacgcc ggtggtgaat gcaggaactc
     1801 tttatcagta aacgatgggt gtcttactat ttcgtgaaga acaaccggtt gggcaaacaa
     1861 tgatccccct ggtgcaggtc ttggtctggc tctttgtgac aggcctgttc ggatggggat
     1921 tttatgagca actggtgctg ggcaaaccct ggggcgataa tcctatgagc gatacagcgc
     1981 tgattgtcat cggaattaca gtgatcatct tcatgggttt aatgaccgta ctgtctatcc
     2041 gaaacagcct tatcaccgaa gtgcattctg acggatttta ttacaggttt cctcctttca
     2101 tcaacaggat gagatgtata ccggttgaac agattgcttc ggtagaagtc aggaaatatg
     2161 gtttgtttcc cggtagtttc ggatggggaa taaagagaag gccgttgcgc aggacaacta
     2221 tctatcaggc gggaggcaat aaagtagtgg tgatcacgct caaaaccgga cggcgcatca
     2281 tatttagtac aaagaaaatg gaagaaatgc gcagggctgt tgacagaatg atggctgaac
     2341 gggataatca caaaacgatt ttctaagtta tccggatgca aaacattcag gaataataaa
     2401 ggatctgcta tgtcgaaaaa gaaagtattt gcattgcgta ttgacgagca gttgctggca
     2461 gccgttgaac ggtgggcagc cgatgatttc cgtagtacga acggacaact ggaatggatt
     2521 atccacaatg ccctgaaaga ggccggccgt cagccaaaac aaacggcgga ggatggaaaa
     2581 cagaaatgac ggtatcagca agtagctttc cggattgaaa aaaagaccct gaaggttttt
     2641 aaaaccttca gggtctgctc tgtatttatc atccgttggg cctactcctt ctttacgaca
     2701 gtgcctttta tctgcagaac tgaaggcgaa gcttcagcgt tggtcgtcac ggttatagtt
     2761 ttcataaaag gcccgaccga tgcggcgtta aatgttaccg aaatgacagc cgatttgccg
     2821 gaaagaatgg gttcctggga gtatttgagg ttggtgcaac cgcaggaagc ttgtgctttc
     2881 tgaattatta acggctcttt cccgtcgttg gtaatggtaa attccgctgt ttttggaatt
     2941 ccccggtcaa tttccccaaa atcgttgatc atcttatcga accgggcttt gggcccgttt
     3001 ggatttgaaa tggctttttc atttgcagta gcatgaacgg atggttgggc attagcccag
     3061 ccgcaggcag caatgacagc taaaaggagg atgatttttt tcataaggtt tgtttttaaa
     3121 attggcaggt acaaaggtga ggctggaatc tgtaaatcaa tcaaaaagaa tgtaaaactt
     3181 ggtaaatcaa atgtaaaaga ttgtaaacga aatgcaaatt atcacatccg cattgatttt
     3241 tacctacttt tattgttaca aagaaacaga tcacccgtat gagaataaag catatccggt
     3301 ttgttgtttt actgggctct tttgctatca tcggaatttt tgccatccag tggtatttcc
     3361 tgaaaaagga atggaataac agggaaaagc agtttacgca aacggtcatg atcggattgc
     3421 gcaacgttgc cgatgtgctt cacaagataa accagacgaa tcctaccacc acaaatccgg
     3481 tcaggcaact gtcatccaac tatttcgtag tggatgtaaa tggcgtcatt gatgccaacg
     3541 tactggagca ttacctgaaa ctggaatttg agagactgaa tatccggacc gattatgaat
     3601 atgccattta taactgctat acgggtgtaa tggagtatgg caactatttt ttctataacg
     3661 ggaaaataaa gcataacgct atatcggtga acctccccaa gtatgacgaa tacaattact
     3721 atttcggggt gaatttcccg ttattgaaaa acaccattgc cggcggaatg accatgtggt
     3781 tttttttgat ggggatactt tttgtcgccg tcatcttttt cggttattcc atttttgtca
     3841 tcctgcaaca gaagaggctt tcagaaatgc agcgggattt catcaacaat atgacccatg
     3901 agtttaaaac gccgatatcc agcatcaata tttcggccga tgtgattctc aaccctgaat
     3961 ccctgatgga acctgtgcgc ctgcaaacat atgcctcggt tatcaaacag gaaaccagcc
     4021 ggttaaacga tcaggtggat aaggtccttc agattgcacg tatcgaaaag cagggtttcc
     4081 atttgagaaa ggaagttttc gatctgcatg tcctcatacg gaatgtggct gagaactacc
     4141 ggaaggattc cggcaggaat atcaatattg atactaacct ggcgccggga accgtggagg
     4201 tgaatgccga caagcttcac gttgccaata tattccataa ccttctggac aatgcaatga
     4261 aatattcggg agagtccccg gaagtcacca tctcctcaag ccttcggggc gggagggttc
     4321 aggtccggtt acgtgataat ggtcccggta ttggccggca gcatcagaag aagatattcc
     4381 ggaagtttta tcgtataccg accgataatg ttcatgatgt aaaaggattc gggctcggcc
     4441 ttttctatgt aaaaaatatc tgtgatgccc atcggtggag gatcagcctt aacccggacg
     4501 aaagaaacgg agccgaattc attctggaaa taccggtttt aaagtcagcc ttatgaaaaa
     4561 tgcccgcatc ctttatgttg aagacgatgt gactctctcg ttcgtaaccc gcgacaacct
     4621 tgaacggtcg ggatatgtgg tggactcctg tgaggatggc ctgcaggctt ccgaaaaatt
     4681 tgttcccggg aagtatgacc tgcttatcct ggatgtcatg cttccgaaga tggatggtta
     4741 caccctggca aaaaaaatcc gggaaaagga tgaatcggtt cccatcctct ttctttcggc
     4801 aaaatcgacc aaggacgaca agatactcgg tttgcaactg ggagctgatg attacattac
     4861 aaaacctttc agtattgaag aactgatcct gaagattgag gttttcctga aaagaagaag
     4921 cattgaccat ggtaatacta tgcaggcgga ttctttccga ataggaagat tcatgttttc
     4981 gtcgtcggga cggcagctca tcctggacga tctgaaacaa acgctcacct acagggaatc
     5041 ggagctcatc acatatttat tccggaacct gaacaaggtg gtaaaacggg aagaaatcct
     5101 gatggcggtg tggggtaacg acaattacct gatcagccgc agcctggatg tgttcatttc
     5161 ccggcttcga aaactcttcc gcggcgatcc ttcgattaag attgaaaata tccacaacat
     5221 tggttatcgg atgtccatta ctgaaagccg ggaatagaag ccgtcttgct ttcccgggag
     5281 aaaccttagc tcgcgtgcat ctcctgacgc gtgcgttgag gttttataat gcttaaaaaa
     5341 ttgtttactt ttatggcatg gttgacgaaa ttatctatcc gaatagctgg tgttcgagaa
     5401 taagtggagc acacgtcagg agtcatgtgc ggaagttagt atagcaacgt aagcgtagca
     5461 catgcgcgcg cgagcgaagg gatttgataa ttcaggtatt tataatagat aaaacttaat
     5521 atatgagaca tatgcttatt tcaataatat gtttgatgct aatatcatcg acatgtaaaa
     5581 aggaggaaga ttgccattcc tatattgatt tcacaaacca atctgaaaaa gacatcattt
     5641 ttggtcttcg tgctacagat tttaaaggga attgtaggct tgatggaaat aaagtaatga
     5701 aaaatgaaac ttttgatttc cgtccatata attcctgtat tgaagataac ttgaatagca
     5761 atacgccatt agatatatac atagtagata gtgataaata taatgaaccc agtgtttttt
     5821 atgactgtga ttcaatagaa ataaaaaata aaatcttaaa acattataaa ttaacgcttg
     5881 aagacctaaa gaagctagat tttacaataa tttacccatg aaactaataa aaggaataat
     5941 attgtgtata gtgctttccg gctgcggtaa aactcccata gcagattttt cctggtcgcc
     6001 tcaaaatcct aaagcaggcg aggaggttca attcaccaat ctatcaactg atgcgaaaga
     6061 gttttcctgg aattttggag atatggacat tagtaatgaa tcaaatccaa aacatacata
     6121 ccagaaaagc ggtaactata ttattgactt aaatgcaaca agtggcttaa aaacagacat
     6181 taagaccgta acgattattg ttacgcctta aaataaacta tttgcccccc gctaccgcgc
     6241 gaacttttct cttcgccgga ggcggaaatc ctttcgcgtg gtgattgata ttctcccttt
     6301 gctcgcgcgc gtctcctgac gcgtgcgctg taaacctgcg gtattactgt atctttatca
     6361 gctcgggtat tgaagatttt caaaaccttc aaggcctgct caaccgtcag tccttctcac
     6421 tgagcgcctg tcccgattca tcgggaagga ggtgccgaag gcggatgaag tgaaaatctg
     6481 cggcaaactg catctttttc agcacgggta ttgaagattt tcaaaacctt cagggtctgc
     6541 ccggccggcc ttctccctga gcgcctgtcc cgattcatcg ggaagaaggt gccgaaggcg
     6601 gatgaggtga aaacttgtgg cattatggca acaaggcgaa attattactg tccggaaacc
     6661 gtttcatacc tgaaaacagg aatatgtaga tatcaatcca gttgcatata caattagtcc
     6721 ggcaacaaat tatgccgtga aacgctcctt gtttgaggta atatcgttaa gtttgtagta
     6781 agtgtagtaa cgcacgcata atatgcgcta gcgggcgaag gttataacaa atttattcgc
     6841 tatggttttc ttttttttac aatccaataa attattcaat aaaactaagt gttttttatc
     6901 tctattgtta attttattat catatggaga tttaaaatca caaaatcaaa aggtatttgc
     6961 taacacagac cttgatacaa ttataatgta cgaaagtact gcgtatctca atttaggtgg
     7021 ctgggtcggg ggagctaaga tttttaaaaa gaataatgag ctagtacttc atacaacagc
     7081 ctattctcaa cacttggaat cattttcaaa aattatctat agaaaatgtg atacttgtaa
     7141 aaaaaatgag ttcattgtta gaatcttcaa tttaaatgag ccagttaata atcaatgtat
     7201 gatatttgga gtagtcaatt atattgattt tggaattgtt gagtttctta acaaaaattt
     7261 tgtggtgttt aatttgactg aaatgccacc tgattcaatg ttacgaataa gtttttgttt
     7321 agaatattat ccattgatgt taaaattgac aaatataagt ggaggtatat acgatattca
     7381 cctatcaaaa agctataaac ctttatcatt atttgaaaat ggcttaataa taagatgtaa
     7441 atacttaaat tcaacctgtt tagagtgctc atataaaatg gaaaatccgg ataaacagat
     7501 aaatttagta attaaaagaa aaaataaatc accaattgta ttactaacaa taaatagaaa
     7561 aagtacatac aggttttctt tatcaatccc taatgtcaat agtatgcctt aaaaacaggg
     7621 cctttgatag taagttaagg ttttgattat caacgcagat accgtcaagc tgctgccgta
     7681 cgattccccg ctgccacgcg aacttttctc tctgccacag gcggaaatcc tttcgcgtgg
     7741 tgattgatat tctccctttg cttgtgcgcg tctcctgacg cgtgcgctgt aaacctgcgg
     7801 cattactgca tctttttcgg cac
//
LOCUS       DAOPYB010000144         7683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_11472_length_7683_cov_8.940220, whole genome shotgun sequence.
ACCESSION   DAOPYB010000144 DAOPYB010000000
VERSION     DAOPYB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11472_length_7683_cov_8.940220"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..1124)
                     /locus_tag="PKH58_10165"
     CDS             complement(468..1124)
                     /locus_tag="PKH58_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89439.1"
                     /translation="MKTKIRTISLMLLGVFILTFWGIKILYINMPKESLKSYKVCHGA
                     GAPTFNSNEFMMKIPAYKEGSTTATRHRPANISVNRQSAGNVYVPIKSVDKAISSGFS
                     MPGTDKSIYSLKSKKSSDENSLNFRPMALMLSLSVKPTGTMNLVTSGKNVESELLASN
                     LSAPFSESNISGPMRMDGDGENPPPEGTPIGNGIWVMLLLVVGYALYRKKLLLDSFKY
                     "
     gene            complement(1237..5385)
                     /locus_tag="PKH58_10170"
     CDS             complement(1237..5385)
                     /locus_tag="PKH58_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89440.1"
                     /translation="MKLKNKLSNKAVLIITIMMMLIPWLPSNAQVKVPFTQRTSAVTP
                     NQKIYNIKGDFQMIGNTNLTLEAYGDNIPNSNNQMIYVDVDNNPQTINSSSAELTFPV
                     ENESLPSCSNIIYAGLYWTGRAHDGESSNTFTVPITVPGSPITVNNNQTPGNNSKITY
                     TNYSLTIVRSGDRNNRTITYTFTSSDTGNTVAFIYAYNSGSPTLSVSVNGATPTAVTT
                     TSINDKDAVFSPYTIYNDAGGIDLTVNHLYRNGKNADINDALAYTNVTGTYTPPQTIN
                     KNLDKSVVYLKHTTETGYTTITASDINFTQNIYYPTTTDGYMYSAYAEVTDYVKQHGM
                     GSYTVSDIALREGDGGSTGFYGGWAMIVVYENSKMNWRDITIFDGHAYIRDEANSNAM
                     TYYELPVTGFHTAQSGAVNMKVGIIAGEGDRSIGGDGTNIDNFSIRNYQDDAWIPLST
                     PDNPVNNFFASSIVTNGVRNPSLYNNTGLDIVNINVPSTALTNNQTSTKFRYGTNQDT
                     YIIPCIVMAVDAYVPELVPYISVGSVNGEPYSPTNSEVLPNGEIEYSLELRNPGTEQL
                     LNAAIDIPIPYTATFVSASAEYFLGTGGTTPQQPVFTISGVSKFIHWNIGTIPKGGTN
                     LLARLKFKLKATGDCFLLVNNNCSPTVLLEGTSSGSGETSGTEFHNLRFIHGYRDGVC
                     QGEPIIGPLSMNIDYEDFVAQNCGTESYLGGAKIYNFCKKPGSSTIDYNLIAINFPVG
                     SRFYNAVVTENIDGVIVVKPAEDATEYTVTTNFPATIGTTAYYAIPPGASTCWWEFEI
                     KVEDCNLWYGGTDSNWGNTANWTQGAIPAPTDNISFATPHNYTGEALSDLILDQNRSV
                     KNITNLSTRKIIIPANITLIVDSLANTGTASQVIIESDITKANGALIFNRPGINPGFQ
                     ATIQFASKSKPGSGARSSAWQFFGVPVVDRTLADLFGPNVQGSIYGGNPAINTIVRKY
                     KESLNLSYSYQEKWEDLDANSVLSPYYGYEITQPIEGTKHNLAGTIVTDDKTLNFTIS
                     PTGVYSRGNYILANPYTAPIFISKMLSSDFVNLAPTIYIFNTGSRQDWLTNNGSSQIG
                     DLPGTYSAIPINAANTIGKTQIPSMQAFMVKAISDAAPATNFKFRYATVNRGTLTYPN
                     EPMKVNRSVETENKDIKPLLMVDVIGENSSDRVYLITAEETGKSYDPGWDGYKSLSTG
                     IVQLYTMDADSKRMQVNTDNDLNDTYIGFRTGGENTYTLRFKFNNEMNDVYESLYIQD
                     LVTGSMQEITDGMTMNFDATAGTAEKRFKISGAKIKTGVITSDIHEAIKVTANSSKIR
                     INNNTNGEVTIKVYNLTGQSVLIGKAAVGVQYISHNLTKGTYILEARSINSGRKKIFK
                     STIH"
     gene            complement(5420..5932)
                     /locus_tag="PKH58_10175"
     CDS             complement(5420..5932)
                     /locus_tag="PKH58_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89441.1"
                     /translation="MINDKKKSFRTKLLSFLLIYFAVMPTFGQVVLSYSNYLKASSDS
                     HLISLVTEIKPSIYMKDNADIRLSSNTAAIVVNTDIASIPMLYADKTDYNKVELLKIR
                     INSDTDEAVKLDLSRLTAFSALKYVLYVYQFNYCGDNKDTCLQATTESKVYTPADTMV
                     KILYLLSIPE"
BASE COUNT         2245 a         1339 c         1544 g         2555 t
ORIGIN      
        1 ttaagtgatg tccagttccc cgaagttatg tttgagcggt agtcgccaac gttttgggct
       61 tgaataccag atacgcataa aaaacttaat actatcagta ttatagtagt tatgcggaaa
      121 ttgtatttgt tgaaattata acctctaatc ataattgtct aatttaagat ttttaattac
      181 agacagatag attaaagaag gatgggaaat tcaatagttc tttctttcga agatgaacta
      241 ttcgataagt aacggttaaa atatttacaa tattagcaat aataaactaa attgattaac
      301 taaaggataa atttaacaca atcatttaac gttttctgaa agaagattct acacgttgaa
      361 ttcctgagaa tgtttttata ggtgtaacat gtaatgtttt tacagtaaaa aatagtcttc
      421 ctggtgatga ttttatgatt agatcccggc tcatctcaaa aatagattta atacttaaaa
      481 ctatccaaca gaagtttttt tcgataaaga gcatatccca ctaccaacaa aagcattacc
      541 caaatcccat ttcctatcgg tgtaccttcc ggtggagggt tttcaccatc gccatccatt
      601 ctcattggtc cggagatatt actttccgaa aaaggagcag acagatttga tgcaagcagt
      661 tcgctttcta catttttacc tgaagtaacc aggttcatgg taccagttgg ctttacactc
      721 agggaaagca ttaatgccat tggtctgaaa ttcagcgaat tttcatccga tgattttttc
      781 gattttagcg aataaatgct tttatctgta cctggcattg aaaagccgga tgaaattgct
      841 ttatctacac tctttatggg cacatataca ttacctgcgc tttgcctgtt cacagatata
      901 tttgccggtc tgtgacgggt agcggtggta cttccctctt tataagccgg aattttcatc
      961 ataaactcat ttgaattaaa tgtaggtgca ccagcgccgt gacaaacttt atatgacttg
     1021 agtgactctt ttggcatatt tatatacaga atttttatcc cccaaaaagt caatataaag
     1081 actccaagca acattaaact tatagttctt atttttgttt tcataccttg ttatttttaa
     1141 ttgtttccac aattgtcttt taaatcaaat caggattatt tagcaagaat acaatcccga
     1201 atttgaatat caggctcgtt tttttttgac ggtattttaa tgtatagttg acttaaatat
     1261 tttttttctt ccggaattaa tcgatcttgc ttctaaaatg taagtccctt tcgtcagatt
     1321 gtgacttata tattgaacac ccacggcagc tttcccgatc aaaacggact gtcctgtcag
     1381 gttgtacact tttattgtta cttcgccatt cgtgttgtta ttaatccgga tttttgaaga
     1441 attagcggtc actttaatag cttcatgaat atcagaggtt ataacgccgg tttttatttt
     1501 agctcccgat attttaaatc gtttctcggc cgtgccggct gttgcatcaa aattcatggt
     1561 cataccatcc gttatctcct gcatacttcc cgttaccaga tcctgtatat agagtgattc
     1621 gtacacatca ttcatctcgt tattgaattt gaacctcagt gtgtatgtgt tttcacctcc
     1681 ggtacggaag ccgatatacg tatcgttcag atcgttgtcg gtattgacct gcattcgttt
     1741 gctgtcggca tccatggtgt aaagttgcac aataccggtg gataacgatt tgtaaccatc
     1801 ccatcccggg tcatatgatt ttcctgtttc ctcagcagtg atgagataca ccctgtcgga
     1861 actgttttcg ccaataacat ctaccataag cagtggttta atatccttat tttcagtttc
     1921 aactgatcta ttgactttca taggttcatt gggataagtc aatgtgccac gatttacagt
     1981 tgcgtatcgg aatttgaagt ttgtagcagg agctgcgtcc gaaatagctt ttaccataaa
     2041 tgcctgcatg gaaggtattt gtgttttacc aattgtattt gcggcattga ttggtatggc
     2101 tgaataggtt cccggcaaat ctccaatctg ggaagaacca ttattggtca accaatcctg
     2161 tcggctaccg gtattgaaga tgtagatggt cggtgccaga ttgacaaagt cggatgaaag
     2221 cattttgctg ataaatatcg gtgctgtata ggggttggca agtatgtaat tgccgcgcga
     2281 atatacgcct gttggcgata ttgtgaaatt aagtgttttg tcatcagtga ctatggttcc
     2341 cgcaagatta tgtttcgtgc cttcaatcgg ttgagtaatt tcatagccat agtaaggtga
     2401 taaaactgag tttgcatcca agtcttccca tttttcctga tagctgtaac ttaaattaag
     2461 agactctttg tatttacgaa caatggtatt gattgccgga tttcctccat agatgcttcc
     2521 ctgaacatta ggaccgaata aatctgcaag cgttcggtct accacgggta caccaaagaa
     2581 ttgccatgca gacgacctgg ctccgctacc cggttttgat tttgaagcaa actgtatagt
     2641 agcctgaaat ccgggattaa taccaggcct gttaaatatc aaggctccgt tagctttagt
     2701 aatgtccgac tcaattatta cctgtgaagc cgttcctgtg ttcgcaaggc tatcaactat
     2761 taatgttata ttcgcaggaa taattatttt tctggttgaa agattggtta tgttttttac
     2821 agacctgttt tgatcaagta ttaaatcact taatgcttct ccggtataat tatgtggtgt
     2881 agcaaaagaa atattatcgg ttggtgccgg tattgccccc tgtgtccagt tagctgtatt
     2941 tccccaattt gaatccgtac caccatacca taggttgcaa tcttcgacct taatctcgaa
     3001 ttcccaccaa caggttgatg ctcccggagg tattgcatag tatgcggtag ttcctattgt
     3061 agcaggaaaa ttagtagtaa ctgtgtattc tgtagcatct tctgccggtt ttactactat
     3121 tactccgtca atattttctg ttactactgc attataaaat cttgaaccaa ctggaaaatt
     3181 aatggctatt aaattataat caatggtgga agatccgggt tttttacaaa aattgtatat
     3241 tttagcacca cccagataag attcagtacc acagttttga gcaacaaaat cctcataatc
     3301 tatattcata gacaagggac ctataattgg ttcgccctga caaacaccat ctctgtagcc
     3361 gtgtataaat ctcagattgt gaaattctgt gcccgatgtt tctccggatc cgcttgaagt
     3421 tccttctaaa agaaccgtgg gtgaacaatt attgttcacc aataagaaac agtctccggt
     3481 tgctttcaac ttgaatttta atcgtgccaa taggttggtt ccacctttag gtatagttcc
     3541 aatattccaa tggataaatt tagaaacacc cgaaatggta aaaaccggct gctgaggcgt
     3601 agtacctccc gttcccagaa aatattcagc tgaagcgctt acgaatgtgg cggtataggg
     3661 gattgggatg tcaatggcgg cattcagtaa ttgttcagtc cctggatttc gtaattccaa
     3721 tgaatattct atttctccat tcggaagaac ttcagaattc gtagggctat aaggttctcc
     3781 attcaccgaa ccaacagaga tatagggaac caattcgggc acataagcat caaccgccat
     3841 gacaatacat ggaatgatat aggtgtcctg atttgttccg tatctgaatt ttgtggaggt
     3901 ttgattattt gttaatgctg tgctgggtac attaatattc acaatgtcga gtccggtgtt
     3961 gttataaagg ctcggattac ggacaccatt cgttacaatg gaactggcaa aaaagttatt
     4021 aacgggatta tcgggggtag atagtggaat ccaggcatcg tcctgataat tacgtatact
     4081 aaaattgtca atatttgtac catctcctcc aatactacga tctccttcac ctgcaataat
     4141 gcctactttc atgtttacag cgccactttg cgctgtatga aagccggtaa ctggtaattc
     4201 atagtaggtc attgcattgc tgttagcctc gtcccgaata taagcatgtc cgtcaaaaat
     4261 cgtaatatcc cgccaattca ttttggagtt ttcatacacc actatcatag cccatcctcc
     4321 gtaaaaaccg gtagatccac cgtctccttc tctcaaagca atatctgata cagtatacga
     4381 acccattccg tgttgcttta cataatccgt tacttcagca taagcagagt acatatagcc
     4441 gtcggttgta gtcgggtaat atatattttg agtaaaattg atatcggagg ctgtaatagt
     4501 tgtgtagcct gtttctgttg tatgtttaag atatactacg cttttatcca gatttttatt
     4561 aattgtttgg ggtggtgtgt aggtgccggt aacatttgta taagcgaggg catcattaat
     4621 atcggcattt tttccattcc ggtacaaatg attaacggtt agatcgatac ctccagcatc
     4681 attatagatg gtatagggag agaagactgc atctttgtca ttgattgatg tagttgtgac
     4741 cgcggttggt gttgccccgt taacacttac acttaatgtt ggacttccac tattatatgc
     4801 gtaaatgaat gcaactgtat tacctgtatc tgaagatgta aatgtatagg taatcgttct
     4861 attgtttcta tccccgcttc ttactatggt taaactataa ttggtataag taattttgga
     4921 attattaccc ggagtttgat tgttattcac ggtaattgga gaaccgggta ctgtaatggg
     4981 aaccgtaaat gtatttgagc tttcaccatc gtgtgccctt cccgtccagt aaagtccggc
     5041 ataaatgatg ttggaacatg aaggtaaaga ctcgttttca accggaaatg ttaattcggc
     5101 cgaagatgaa tttatagttt gcggattgtt atccacatca acataaatca tttgattatt
     5161 ggaattggga atgttatctc cgtaagcctc cagtgtcaga tttgtattac caatcatctg
     5221 gaagtcgccc tttatattat atattttctg atttggggtg acagcggaag tacgttgtgt
     5281 aaatggaact tttacctgtg catttgaagg cagccatggt ataagcatca tcatgatagt
     5341 tattatcaaa accgccttat tggataactt gttcttgagt ttcataataa ttatttctta
     5401 aaggtttagg tgtatctggt tattccggta tggagagtaa gtatagtatt tttaccattg
     5461 tatcagctgg tgtatagact ttactttcgg tagtagcctg caggcaggtg tctttattat
     5521 ctccgcagta attaaactga tatacataaa gtacatactt tagagcacta aatgcggtca
     5581 gtcgcgataa atccagtttt acagcttcat cagtatcact attaatcctg atttttaaca
     5641 attcaacttt gttgtagtca gttttgtcgg catacagcat gggaatggat gcaatatctg
     5701 tattgacaac aatagctgct gtattactac ttaatcttat gtctgcgttg tctttcatgt
     5761 atatgcttgg cttgatttcg gtcaccagcg atatcagatg actgtcgcta cttgctttca
     5821 gatagttact gtagcttaat actacttgtc cgaaagtggg catcacggca aaataaatta
     5881 aaaggaaact tagtagtttt gtccgaaatg attttttttt atcgttaatc ataggttggg
     5941 tgttttagat tttttgataa tgatttattt ttatagaagt tgaaactaca cgttgatcta
     6001 ttaatggttc tactgttgca taatagttga ttgctccgct gtgagctgac ttgcaaatca
     6061 atattgcaga ctggaaacaa gaggtatgat ttatgaaagt aatgctaaag agagatgagg
     6121 tcaatacact tatgtttgga cattgtgtac ctgaaggaaa tgaatgtgtc aaatcagtga
     6181 aaagaattga agaagttaat gttcgggact ttttatctgc cagagtgcat tttttgtcaa
     6241 aaatcgaaga gttaaaataa tgatagggta agttgtaaaa tgtaatgctc ttattaatag
     6301 ttttattccc tgttggaaag gaataaatag atgatactac agaattgtat gcgaaagctg
     6361 tgtcaatatt aatatcacga ttactaatct taatatttaa attgtttatt ccaagaggtg
     6421 gaatgaaggg agtgtgtaaa tgtatttttt gatagaattt aatagttatc cctgaactga
     6481 cccctgaacc gacaatttta ggtgacaggg tggcaggtgt tgttgtatta taatgggtag
     6541 aactgaaggt ataatcttct gtgattgtag tacacagagt taatatacta gagagtgaac
     6601 caagggcgag tatgaagacg aagagtggaa tgaatatccg actctttggt aaagagctta
     6661 atgtattcat cataaataag tatttaagtt aaaagtaaag taaacagtgt aaacagaaga
     6721 tcgttgtaaa cgaacatcaa aatataagaa acacctttct tatttgggat cagtagatta
     6781 tatgcaatct gttatcatca aacatatgtg gattgtatag attgcaaact taaacataat
     6841 atttttaaaa gatatgttta tttctaaaaa aattaaagtt taacataatt taatctaaca
     6901 aattaagttg ataacaattg aagagagccg ataattttaa tttataccct tcattaatct
     6961 tccggaaaca atttttccga tataaaacct tgattacaat tgccgggagt gtccaagaaa
     7021 ctgtgtaaat tgaaaaagtg atttgcgatg agcgatgaga ggtgagcgtc cagagcaaag
     7081 agccagaaac caggactttg agacagggag aggtgtaaat agtgatgagt gataatgtta
     7141 tgatttgaag atttgaggat ttgaagattg tttattgatg aataggggag aggggatgat
     7201 tttgtaagcc gcgggagtga ttatgtatgc cgcggtcata aaaatgtaag ctgcggtcat
     7261 aaaaatgtaa gctgcgggga taaaaatgta aggagcggga atggttttgt aagctgcggg
     7321 caaggttttg taagctgcgg ggaaggtttt gtaagtcgcg gggaaggttt tgtaagtcgc
     7381 gggaataaaa atgtaaagag cggaatagtt ttgtgattgc ttcgacagga cttgacctcg
     7441 actacactca gccaaactta atttattttc actctgtggc aagaaatatt attttgaacc
     7501 aatgtaaaat acaacctctt ttattatatc tactccacct gctttttaca ttgatccaca
     7561 taatacttgt ctcttgaggg actgaaaaga atatagacgt agtacataat tatccggcaa
     7621 atcaaaaaaa ggctgcaacc tgcaaccttt ttgtagcgag agccggggat gatccggcga
     7681 cct
//
LOCUS       DAOPYB010000145         7638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_11575_length_7638_cov_9.653831, whole genome shotgun sequence.
ACCESSION   DAOPYB010000145 DAOPYB010000000
VERSION     DAOPYB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11575_length_7638_cov_9.653831"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..673
                     /locus_tag="PKH58_10180"
     CDS             161..673
                     /locus_tag="PKH58_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89442.1"
                     /translation="METEINNKIAEKATEQADKNENSLRITPTSKDNLISTSRWANFI
                     AIVGFVMLGIMVVISISSFIITPIMGEFQDFQSIPFPLYLFGLIYLIYAVIGFFPYYF
                     LYTFAKKVRKGLENNDQDTFDMGLINLRRLALFVGVLMIIALSMLLTIIPIIVFSFGM
                     LHTLSGGTMI"
     gene            complement(724..1548)
                     /locus_tag="PKH58_10185"
     CDS             complement(724..1548)
                     /locus_tag="PKH58_10185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX89443.1"
                     /translation="MKKIIRISILISIIAMMEGCSTTKNTETRVKMQTTYGDISVKLY
                     NETPLHRDNFLKLVNSGFYNGVLFHRVIADFMIQGGDPDSKTASPTDSLGNGDAGYTV
                     LAEFKTPAIFHKKGALAAARLGDAENPLKASSGCQFYIVEGKTFSDAELDNMQTNKLR
                     QQENALFQEILKTKNDEVKKYRLERNQEKLDQLRDSILLEVKNKISADSTYKFSDKQR
                     TIYKTLGGTPHLDGNYTVFGEVTEGIEVVEKISKTRTGRMDRPIEDIRILKMEVVK"
     gene            complement(1578..2330)
                     /locus_tag="PKH58_10190"
     CDS             complement(1578..2330)
                     /locus_tag="PKH58_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNX89444.1"
                     /translation="MKHNFLDLSKTNWGEVQQHQYDIVVLPWGAIEPHNFHLPYITDA
                     IISSEIACKSVEQAYAQANVSAVVMPPIYLGSQNPGQWELPFCIHASYETQKAILTDI
                     VKSLYGQGFRKLLIINGHGGNSFKSMMRDLAVTFSDFYIISSDWYNFVPRQGFFDEDI
                     DDHAGEQETSVMMYFHPELVDLNTAGNGTPNPTKMKSIANKTGWMPRNWKETTLDTGV
                     GNPKKSTAEKGERYVNEVVNRITEMLIEFKEM"
     gene            2470..3828
                     /locus_tag="PKH58_10195"
     CDS             2470..3828
                     /locus_tag="PKH58_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX89445.1"
                     /translation="MGKIYEFTPPYMLVKSYMLFGFKRFYREFIVNGKENLPVDGSPL
                     IFAPNHLNALMDALAVSTLVPRTKAVIYLARADLFAQKAIAKIMHFAKIMPAFRMRDG
                     FDNLEKNNRVFYQCVDVLRFSHSLCIMPEGGQGEERKIRPLVKGIFRVAFEAQQQFGE
                     LKKVKIIPVGINPADLEKSGKPIIINVGKPIEVSDYIASYLSNPVATTNEIRDELKQR
                     LTDLTLNFETNRYYKSFETISEIVSHNYIEPDKKANSTLEEFILKQETAGKLIRIERE
                     DPEKMKELDRLAKNYNKIISKLNFKSAIFSQQKKYEPTLGLFFRLLAGLPVFLFGFLT
                     NFIPASTAVLLRKLFKVEYSGFFSSVDFALSLIIFPLFYILQTILTANLFHMTWWLIL
                     LFFILQFLTRQPAVKWYSRTRKFIARLRFDSLLVQRSHASNLLYRAISVRNKIIYKLN
                     QS"
     gene            complement(3904..4359)
                     /locus_tag="PKH58_10200"
                     /pseudo
     CDS             complement(3904..4359)
                     /locus_tag="PKH58_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760417.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
     gene            complement(4463..5224)
                     /locus_tag="PKH58_10205"
     CDS             complement(4463..5224)
                     /locus_tag="PKH58_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89446.1"
                     /translation="MTGLMRGAIGVEITLQRGQTSYRLPDMPELEGKQIISVNYCNAN
                     DVPRTSSGKIVFPLSSDRLELCLADINTGMTCKYSVADIENSPVHLTFDQKVDMQTSE
                     LALVNSDNPDIWLDTTVYLIFWYGINTDNFQFSKPRILPLEVTVNSPKNFFQPQFTLL
                     NSRPLQLMLTFPGFTPSGRKAIDRSSVSDKFITLADKDKMLFSQIPAYYFIQSHLKTP
                     WFFDLPSIDFEKSYVKSLKDDFSKAEILFFNLMCV"
     gene            complement(5221..6078)
                     /locus_tag="PKH58_10210"
     CDS             complement(5221..6078)
                     /locus_tag="PKH58_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89447.1"
                     /translation="MFRLNTSPKPLTIGISTGKAGQDKRRNIRTNTAIPEKMIMPHEL
                     QNIACFEITVPTGTNIISLGHVNLNDRLIQSVYLFSSTEDVMLFSPVSGKQVSALAET
                     GPCAIYLNLTDTDNELFVRDYSSGNFTIYSEMDHFVSWRINRKIDWNQSFLKTIIPDK
                     ASTLNLLMYIAFENVASKDLSDREVVEKTVNLIPENDIQDFCLDVYFPELSDKTITGI
                     YVSNQCNGFFDLYGQNNRLENVPAHFFAIKSSKGFLIEPMKVDLKKSFYRHRSFSREP
                     YRITLTYLP"
     gene            complement(6008..7546)
                     /locus_tag="PKH58_10215"
     CDS             complement(6008..7546)
                     /locus_tag="PKH58_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNX89448.1"
                     /translation="MLVKTFGAAVQGINATVVTIEVNVGQGIRFMLVGLPDNAVRESH
                     ERIASALDYTGQKMPHKQVIVNMAPADIRKEGSAYDLPLAIGILAGDGKIPCEELDKY
                     IIMGELSLDGSLQPIKGVLPIAIKAREEKFKGFILPKQNAREAAVVNNLDVYGVENIT
                     EVIDFFKGSSALEPTVVNTREEFYQNLNLSEIDFNDVKGQDNVKRALEVAAAGGHNII
                     MVGPPGAGKSMLAKRIPTILPPLTLHESLETTKIHSVAGNIDSNTSLITQRPFRSPHH
                     TISDVALVGGGTFPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRKICISRAK
                     FAIEYPASFMLIASMNPCPCGYYNHPTRECVCAPGVVQKYLSRISGPLLDRIDIHIEI
                     IPVPFEKLSEMKESETSQQVRERVIKARQIQELRFHDVDGVYCNAQMSSKQLRTYATP
                     DTEGNALLKNAMLRLNLSARAYDRILKVSRTIADLDNSENVQAQHIAEAINYRNLDRE
                     GWAG"
BASE COUNT         2171 a         1552 c         1528 g         2387 t
ORIGIN      
        1 tcaaaatttg ttgcttcgaa aatatcgaat taataatatt taatttacct ttaccctttc
       61 tcataaaagt tgcattattt tacagattag tttcgattac ttttcaggcc agaacaatta
      121 aaattatcaa tagattaata caaactaata attatgcatt atggaaactg aaatcaacaa
      181 caaaattgct gaaaaagcca cagaacaggc tgacaaaaat gaaaactctc tcagaattac
      241 tcccacaagt aaagataacc tgatatcaac ttcccgctgg gcaaatttca ttgccatcgt
      301 cgggtttgta atgcttggga ttatggttgt aatcagtatt tcttctttta taatcacccc
      361 aataatgggc gaatttcagg attttcagtc catacccttc ccactctatc tgttcggatt
      421 gatatatctt atttacgcag taatcggatt ctttccctat tacttccttt atacatttgc
      481 caaaaaggtg agaaagggtc ttgaaaacaa cgatcaggat acgttcgaca tgggattgat
      541 taatcttcgc agactggctc ttttcgtggg agtactgatg attatagcat tgagcatgct
      601 gttgacgatt atcccgataa ttgttttttc attcggaatg ctgcatacac tttctggcgg
      661 aacaatgatc tgaaaaatat cattaaataa aaaagctgcc cgtttgagca gctttttttg
      721 taattatttc acaacttcca ttttcagtat ccggatgtct tcaattggtc tgtccattcg
      781 tccggttctt gttttggata ttttttccac cacttcaatt ccttctgtca cttcgccaaa
      841 cacagtgtag ttaccgtcga ggtgaggagt acctcccagt gttttgtaga tggtccgttg
      901 cttatcactg aacttatagg tcgaatcggc tgaaatctta tttttcactt cgagcaggat
      961 gctgtcacga agctgatcca gtttttcctg attacgttcc agcctgtatt tttttacttc
     1021 gtcgtttttg gtttttaata tttcctgaaa caacgcattt tcctgctgtc gcaacttatt
     1081 ggtttgcata ttgtccagtt cagcatccga gaatgttttc ccttcgacga tataaaactg
     1141 acaaccagat gatgctttca gaggattttc ggcatcgccc aggcgagctg cagcaagagc
     1201 tccttttttg tggaaaattg ccggtgtttt gaactccgcc agaacggtat atccggcatc
     1261 gccatttccc agcgaatcgg ttggtgaggc cgttttcgaa tccggatctc caccctgaat
     1321 cataaaatcg gcaattacac ggtggaaaag tactccgttg tagaatccgc tgtttaccaa
     1381 tttcagaaaa ttatcgcggt gaagcggcgt ttcgttgtac agcttcacgg aaatatctcc
     1441 gtaagttgtt tgcattttta cccttgtttc ggtatttttt gttgttgagc atccttccat
     1501 catagctatg attgatatta atattgatat tctgattatt ttcttcattt gtaattattt
     1561 gattgtatgg ttgtttatta catttcttta aactcaatca gcatttctgt aatccggtta
     1621 acaacctcat tcacatatcg ttctcctttt tctgctgttg attttttagg atttccaacg
     1681 ccggtatcca gcgttgtttc cttccagtta cggggcatcc agcctgtttt attggcaatc
     1741 gatttcatct tcgttggatt gggtgttccg ttacccgctg tgttaagatc aaccagttcc
     1801 ggatgaaaat acatcataac tgaagtttcc tgttctccgg catggtcatc gatatcttca
     1861 tcaaaaaaac cttgacgggg tacaaaattg taccagtctg aggaaatgat ataaaaatcg
     1921 gaaaatgtta ctgccagatc acgcatcatg gatttgaacg aattaccacc atgcccgttg
     1981 ataattagca gtttccggaa accctgtccg taaagcgatt tcacgatatc agtcaatatt
     2041 gctttctggg tttcgtaact ggcgtgaatg caaaagggca attcccattg tccgggattt
     2101 tgagatccca aatatatggg cggcatcaca acagccgaca catttgcctg agcatatgcc
     2161 tgttcgactg atttacatgc aatttctgaa ctgataatgg cgtcagtaat atagggtaaa
     2221 tgaaaattgt gaggttcaat ggctccccag gggaggacaa ctatatcgta ctgatgctgt
     2281 tgtacttcac cccagtttgt tttggataag tctaaaaaat tatgcttcat gattttttct
     2341 cagttgcgag aaccaaatta aaaacattca attcttgttt tgaaattaaa ttcaaagata
     2401 ttcatttttc catttatgat tggttagaaa aagtttattt tattaaattt gcaggtaaaa
     2461 tcagttatta tgggcaaaat ttatgaattc acgccaccat atatgcttgt aaaaagctat
     2521 atgttatttg gcttcaaacg cttttacagg gaatttatcg ttaacggcaa ggaaaatctg
     2581 cccgttgatg gctcgccctt aatatttgcc ccaaaccacc tgaacgcgct gatggacgcg
     2641 ctggctgtat caacattggt accacgcacc aaagcggtaa tctatctggc acgtgccgat
     2701 ttatttgctc aaaaggccat tgccaaaatc atgcattttg ctaaaataat gcctgcattc
     2761 aggatgcggg atggattcga caatctggag aaaaacaacc gggttttcta ccagtgtgtg
     2821 gatgtactgc gattcagcca ttcgctctgc atcatgcccg aaggtggtca gggcgaggaa
     2881 cgaaaaatcc gtccgctggt aaaaggtatt ttccgggtag ctttcgaggc tcagcaacaa
     2941 ttcggtgaac tgaagaaggt caagataatt cccgttggaa ttaatccggc cgatctcgag
     3001 aaatcgggaa aaccgattat cataaacgtg ggaaagccta tcgaggtttc cgattacatt
     3061 gcttcatacc tgtcaaatcc ggttgctaca accaatgaaa tacgcgatga actaaagcag
     3121 cgtttaactg atctgacact taattttgaa acgaacagat attataaaag ttttgaaacc
     3181 ataagtgaaa ttgtatcgca caactacatt gagccggata aaaaagcaaa cagcacgctg
     3241 gaggaattta tactgaaaca ggaaaccgcc ggaaaactca ttcggattga aagagaagat
     3301 cctgagaaaa tgaaagaact ggaccgactg gctaaaaatt acaacaaaat tatttctaaa
     3361 ctcaatttca aatcagcaat tttcagccag caaaaaaaat acgaaccaac cttagggttg
     3421 tttttcaggt tacttgccgg tttaccggtt tttctgtttg gttttcttac caatttcata
     3481 ccggcatcaa cagccgtatt actccgaaaa ctatttaagg ttgaatattc cgggtttttt
     3541 agttctgtag attttgcact gagtttaata atatttcctc tgttctatat cctgcagact
     3601 atcctgacag caaatctgtt tcacatgacg tggtggctca tactgctttt tttcatattg
     3661 cagtttttaa cccgtcaacc ggccgtgaaa tggtattcgc gtacgcgaaa atttattgcc
     3721 cgcttacgct tcgacagcct gctggtgcag cggtcacatg catcaaatct gctttataga
     3781 gcaatatcgg tacgaaataa gattatttac aaattaaatc aatcataaga gaaagctaaa
     3841 cagatttctc tcttttgtat taattatgtg aaaccagttt tcagtttgtt attttgcaaa
     3901 atactaaaca agcatccaaa catccttttt ctgctcgctg atatatccgc tcattagggc
     3961 tatagtagcc tcatcgttta tttcggaggc agcggaaagt atttcgcgtt ccagactgag
     4021 cagatatcta aaggtattca gcacatattg aagcgtttct tctccatcgg tcacaagagt
     4081 tgtttcctca atttttgcca gtttcagata ccgactgaaa ttatgtatcg gcaattcacc
     4141 aagcattaga attcgttcag caacttcatc ctctttttcg gcagtattgt catacatact
     4201 ttcaaactgt ttgtgaagta catagaaatt ttagcccttc acgtcccagt ggaaacctct
     4261 caaattagtg taatagacct gtaaatcggc aagtaactgt tgcaaaccgt taactacttt
     4321 tattgtgttc tttactccgg tataagctaa tgttttcata taattattta tttagttgtt
     4381 attatttatt tttgataatg caaatttaat ccggaaaaaa tataaaaaca actgatttaa
     4441 tctatgcttt gatagatatt ttttatacac acatcagatt aaagaaaagg atttcggctt
     4501 tggaaaaatc atccttcaat gatttgacat acgatttttc aaaatcaatt gaaggcaaat
     4561 caaaaaacca gggcgtttta agatgtgact gaatgaaata atatgcaggt atttgtgaaa
     4621 acagcatttt gtccttgtcg gcaagtgtga taaatttatc gctcaccgac gagcggtcaa
     4681 tcgcctttcg tcccgagggt gtaaatcccg ggaacgtaag catcaattgt agcggacgac
     4741 tgtttagaag ggtaaattgt ggttgaaaaa agttttttgg agaattgact gttacttcaa
     4801 gtggtaaaat acgcggtttt gaaaactgaa aattgtcggt attaattcca taccagaaaa
     4861 taagatacac cgtagtgtca agccagatat caggattatc agaattgaca agtgccagtt
     4921 ccgaagtctg catatcaact ttctgatcaa atgtgaggtg aacaggacta ttttcaatat
     4981 cggcaacgga gtatttacat gtcattcctg tgttaatatc tgccagacaa agttcaagcc
     5041 ggtcggacga cagtggaaag acaattttcc cggatgaagt tcggggaaca tcatttgcat
     5101 tacaataatt tacagaaatg atctgtttgc cttcaagctc aggcatatcg ggcaagcgat
     5161 aggaagtctg gccccgctga agggtgattt ccaccccgat agcgccacgc attaatccag
     5221 tcatggaaga taagttaaag taatacggta tggttcgcgc gaaaaactac ggtgacggta
     5281 gaaagatttt ttcaaatcca ccttcatcgg ctcaatcaga aaccctttag aggattttat
     5341 tgcaaaaaaa tgagcaggaa cattttcaag acggttgttt tgaccgtaca aatcaaaaaa
     5401 accgttacat tggtttgata cgtagatacc ggttatagtt ttatcgctta attccgggaa
     5461 ataaacgtcc agacaaaagt cctgtatatc gttttcagga atcaggttta cggttttctc
     5521 aaccacttcc ctgtctgata aatcctttga cgcaacattt tcaaatgcaa tgtacatcag
     5581 caaattcaaa gtcgatgctt tgtcggggat tattgttttc aaaaaacttt ggttccagtc
     5641 aatctttcgg ttgatacgcc aggagacaaa atgatccatt tcggagtaaa ttgtaaagtt
     5701 accggaagaa taatccctga caaatagttc gttgtctgta tctgtcaaat tcagataaat
     5761 agcacaggga cctgtttcag ccaaagcaga cacctgcttt ccacttacag gtgaaaacaa
     5821 catgacatct tcggtggatg agaacaaata taccgattgt ataagtctgt cgttcaaatt
     5881 tacatgtccc aatgaaatga tattagttcc ggtcggaaca gttatctcaa aacaagctat
     5941 gttttgcaat tcatgaggca taatcatttt ttcaggtatt gctgtattag tccggatgtt
     6001 acggcgttta tcctgcccag ccttcccggt cgagattccg atagttaatg gcttcggcga
     6061 tgtgttgagc ctgaacattt tccgaattgt ccagatcggc aatcgttctg gaaactttca
     6121 gaatgcggtc ataagcacgg gctgagaggt tgaggcgcag catggcattt ttcagcaatg
     6181 cgttgccttc agtgtcggga gtggcgtagg tacgcaactg ttttgacgac atttgagcat
     6241 tacagtacac cccatccaca tcgtgaaaac gcagttcctg tatctgtcgg gccttgataa
     6301 cccgttcgcg tacttgctga ctggtttcgg attctttcat ttctgagagt ttttcgaaag
     6361 gtacaggtat aatttcaata tgaatatcaa tgcggtcgag caaaggccct gaaattcggc
     6421 tcaaatactt ttgaaccacg cccggcgcac agacacattc gcgggttgga tgattgtaat
     6481 agccgcacgg acacggattc atggatgcaa taagcataaa gctggcagga tactcgatag
     6541 caaactttgc ccgtgagata catattttcc ggtcttcgag aggctgtcgc atcacttcga
     6601 gcacagtcct tttaaattcc ggaagttcat ccagaaaaag tacgccatta tgtgccagag
     6661 atatttcacc cggttgcggg aaagtaccgc ctccaaccag agccacgtcc gaaatggtgt
     6721 gatggggact ccggaatggt cgttgtgtaa tgagcgatgt attgctgtcg atatttccgg
     6781 ctacagaatg tatcttcgtt gtttcgaggg attcgtgcag agtgagcggt ggcaaaatgg
     6841 ttggaatccg tttcgccagc atggatttac cggcacccgg agggcctacc atgatgatgt
     6901 tgtgacctcc tgctgcagcc acctcgaggg ctctttttac attatcctga ccttttacgt
     6961 cattgaaatc aatctctgag agattaagat tctgataaaa ctcttcgcgt gtatttacaa
     7021 cagttggttc gagggcggaa ctgcccttga agaaatcaat tacttccgtt atattttcaa
     7081 ctccgtaaac gtcgagatta tttacaacag cagcttcgcg tgcattttgt ttaggcagta
     7141 taaatccctt gaatttttct tcacgggctt tgatagcgat agggagcacc cctttgatgg
     7201 gttgcagact tccatcgagt gaaagttcgc ccataatgat atacttgtcg agctcttcgc
     7261 atggaatttt tccgtcaccg gcaaggatgc cgatagccag cgggaggtca tacgccgaac
     7321 cttctttgcg tatatcagcc ggagccatat tgacaattac ttgtttgtga ggcatcttct
     7381 gacctgtata gtcaagtgcc gaagcaatcc tttcatgact ttcacgaact gcgttatccg
     7441 gtaaaccgac gagcataaaa cgtattccct gtcccacatt gacttcgata gttacaacgg
     7501 tggcatttat accctgtaca gctgcgccaa atgttttaac aagcatatct gttaatcgta
     7561 tataaaatta atgttctatt cttattttga tggtcttaca aagataagaa attgttctat
     7621 aatacaagtt aaaagcca
//
LOCUS       DAOPYB010000146         7619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_11621_length_7619_cov_9.315970, whole genome shotgun sequence.
ACCESSION   DAOPYB010000146 DAOPYB010000000
VERSION     DAOPYB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11621_length_7619_cov_9.315970"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKH58_10220"
     CDS             <1..614
                     /locus_tag="PKH58_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX89449.1"
                     /translation="PNGDSKLYMIPGLIAILLQIQALILSALAIVREREQGTMEQLIV
                     TPVRSWELMLGKIIPYLVVCIFNLFALLWLSQLFFGVSVAGSLGVLIGLSIIFILGSL
                     GMGVLISTISQSQMQAMYILLFVVLVPAIILSGLMFSRENMPAFTFWFSEMLPVTQYL
                     EITRGIMVRGVEASSLFFSSTLPLIILSVVYFVASVLVFRKRI"
     gene            637..1959
                     /locus_tag="PKH58_10225"
     CDS             637..1959
                     /locus_tag="PKH58_10225"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX89450.1"
                     /translation="MKHNLLKVVSLAVVLTFLLSACATAETGEFTASGSFSAIDTSIA
                     PELSGKVTEVSVLEGESVTAGQTLFKINAEVSQAQYDQAAAAVGAAEAAVQASREQAA
                     SAEAQYQLALQGALVQDMPNYQIAWTNTPPEEYREAWYFNKTELIEAAQSQVESAEKA
                     LASTCAELENEQKKASSRDFIAAEKRLALAQVTLTVAGATLTQAQTAGNTNLEDAAIE
                     SRDAAQAEFDSALSDYQRMLTTSVADSIIMARSRVAIAQATLDNARNALLALQTGDQS
                     LQVVAAQKAAEAAASTVKQAEAGLEQAKKALNMAQLLLDRTTVTSPVDGVVLTRNVEV
                     GDLVVAGGPVMRVANLDTLDLIVYLPEDQYGRVNIGDKVAITVDSFAGETFKGTIVHI
                     SDEAEFTPKNVQTESGRKSTVYAVKIQVQNLDHRLKPGMPADVEFSSK"
     gene            complement(2708..3001)
                     /locus_tag="PKH58_10230"
     CDS             complement(2708..3001)
                     /locus_tag="PKH58_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89451.1"
                     /translation="MDPKTGEVIFVEVERDVSKEQISRKIKWMKFYEASNGNLYVFCD
                     NLNCQRAIQGEINLALSGLNYNSFLTNLHGLRNGKRAGKDGSIWFSQRRANEK"
     gene            3218..4513
                     /locus_tag="PKH58_10235"
     CDS             3218..4513
                     /locus_tag="PKH58_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNX89452.1"
                     /translation="MTHNKHKFSIILIILVFITILSACTTPTSVKATPVRIKMWSIGV
                     EADATHNAFANAISTYNAKHQDVQIDITYIESESFKTQLQVAIAAGDPPDIFQDWGGG
                     VLKSLVDAGVVREIEALNGDASNIWQPAALGPSTFNGKHYSVPVDIVFVSFFYNKDLF
                     AQYDVELPTTWPKFLAACKSFRDVGIIPAVVGNKERWPGALYTDYLINRIGGQEAYER
                     ATYDQANGGSFTDPTFVQAWTKIQEASKAGCFEEGVNSGLSSDAGTLLMTGRAAMQLQ
                     GNWMTYYYRGIDKVFTDNTLDILAFPSVDGGIGNPADLMGGTGQAMVISTKAPPEAEA
                     ALIEMLGSRQFAKDLADAGLIPSIRGSEDLLSNDPLATKMLKMLDTAPSLHLLYGTDL
                     PPKLIDEYWNSAQQILDLAITPEQAAANMQAVAESLNNK"
     gene            4792..5709
                     /locus_tag="PKH58_10240"
     CDS             4792..5709
                     /locus_tag="PKH58_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNX89453.1"
                     /translation="MDIANSVNIKHRKKYTEASVTAVLFLLPATILFLVFVVWPIVQS
                     AHYSVYSWDGLGKPEHFIGLENYARLLKDPVFWKALGNNFFVLIWSLVTQIPLGILLA
                     ILLTGRIKGSVFFRTLYISPMVLSGVIVGLLWQWVYNPSFGLANSFLTWIGFEQYTSG
                     WLGDAGIVMVCVMIVSTWRDLGFYIIIFIVAIQSIPEEICAAAKVDGANSWQLHRYVT
                     LPLIKKTTLTAFVLSLIGSLQFFDLTWVMTQGGPYHSSEVVTTYMFKTAFQLRDWGYA
                     TTLAFVLFCITFFIVIVFLIFNQTRKRFK"
     gene            5724..6560
                     /locus_tag="PKH58_10245"
     CDS             5724..6560
                     /locus_tag="PKH58_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNX89454.1"
                     /translation="MKKLLFHLSIWWQYAIALLFSFFFGFPLIWMFYSSLKSSRELIS
                     NIWALPTHPTLNAYGLVLEVPEFKLYYLNSIVITVISVILLTFLAAMAAFVFARIHFS
                     GSQVLFYFFLAGMMIPPHVTLIPLYSMLREMGLLNKLISLLFPYVGFGLPVSIYILRV
                     YFEQIPLELEDAARIDGASTVRIFWSIFLPISQPALLTVIILSVISVWNEYLFALTLV
                     SGNNQSYTLPLGVLSMVISFSSLQYDRALSALSLTALPVLFFFFLMHRQIIKSLVAKT
                     LN"
     gene            6581..7390
                     /gene="erm"
                     /locus_tag="PKH58_10250"
     CDS             6581..7390
                     /gene="erm"
                     /locus_tag="PKH58_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HNX89455.1"
                     /translation="MVRKRIQLAQNFLKSSSLIWKLISKTSIDDQDIVYEIGAGSGII
                     TSELIKKAGKVIVIELDKNLIDKLIDIFGQNSKVEIHQGNFLEYQLSSPKYKVFSNIP
                     FNITSDIVRKLLYSSNTPEDAYLILQQEAAMKFSGNPTETEFSILAKPWFTFESVWDF
                     QRTDFFPVPSVNVSLLHIHKRETPLVSHENADLYRLFVKYGFGAWKKDLKTAYKDIFS
                     YEQWKRLSKDNRFPIKAIPTQLTFEQWMKLFEFLLKSVPPNKRNNLNKLHK"
BASE COUNT         2142 a         1603 c         1576 g         2298 t
ORIGIN      
        1 acccgaacgg cgactccaag ctctatatga tccccgggtt gatcgccatt ttattgcaaa
       61 ttcaagcctt gatcctttct gcgctggcaa tcgtacgtga acgcgaacag ggcactatgg
      121 agcagttgat cgtcacccca gtccgttctt gggaattaat gttaggtaag atcatcccgt
      181 acttggtggt gtgcatcttc aacctgtttg ccctgctctg gttgagccaa ctcttctttg
      241 gagtcagcgt ggcgggcagc ctgggcgtgc tgatcggatt aagcatcatc ttcatcctcg
      301 gttcgctggg gatgggtgta ttgatctcaa ccatatccca gtcgcagatg caggctatgt
      361 acattttatt gttcgtggta cttgtgccgg ccattatcct ctcggggctg atgttctcgc
      421 gagaaaatat gccagccttc accttttggt tcagtgaaat gctgccagtc acccagtacc
      481 ttgaaatcac acgcggaatc atggtgcgcg gcgtggaagc cagctcgtta ttcttctcgt
      541 ccacgttacc gctgatcatc ctgagtgtcg tctatttcgt cgccagcgtc ctggtatttc
      601 gaaaacgcat ctaacttgcc catcaaggag aaccctatga aacacaattt gcttaaggtg
      661 gtcagtctgg cagtcgtact cacattttta ttgagcgcct gcgccaccgc cgaaacagga
      721 gaatttaccg cctcaggctc gttctcggcc atcgacactt ccattgcgcc cgagctcagc
      781 ggcaaggtca ccgaagtcag cgtgctggaa ggcgagagtg tcactgccgg gcaaaccctt
      841 ttcaagatca acgcggaagt cagtcaggca caatatgatc aggctgccgc agctgtgggc
      901 gccgccgaag ccgccgtgca agccagccgc gaacaggccg ccagtgcaga ggcacagtac
      961 caactggccc tgcagggcgc gctcgttcag gatatgccca actaccaaat tgcctggacc
     1021 aatacccccc ccgaagagta ccgcgaggcg tggtatttca acaagaccga attgatcgag
     1081 gctgcccagt cccaggtgga aagcgccgaa aaggcgttgg catccacgtg cgccgaactt
     1141 gagaatgagc aaaagaaggc cagcagcagg gatttcatcg cggctgagaa aagattagct
     1201 ctcgctcaag tgaccttgac agtcgccggt gcaaccttaa cccaggcaca aactgcgggt
     1261 aatacgaacc ttgaagatgc cgccatcgaa tctagggatg cagcacaagc tgagtttgac
     1321 tctgcccttt cagattacca gcgtatgctg accacttccg ttgcggattc catcatcatg
     1381 gcgcgttcac gcgtggcaat tgcccaggca acgttggata acgcccgaaa cgcgttgctt
     1441 gccttgcaaa ccggcgacca atccctgcag gtggtggccg cccagaaagc cgctgaagca
     1501 gctgccagca cagtgaaaca agccgaagcg gggttggaac aagcgaaaaa agcgctgaat
     1561 atggcacaat tactattgga ccgtacaacg gtcacctccc cggtggacgg tgtagtgcta
     1621 acccgcaacg tagaagtcgg cgacctggtt gtggcaggcg gcccagtgat gcgcgtcgca
     1681 aaccttgata ccctcgacct gatagtctac ctgcctgaag atcaatatgg acgagtcaat
     1741 atcggcgata aagtggctat caccgtggac tcgtttgccg gtgaaacttt caaaggtaca
     1801 atagtccata tctctgatga agctgaattt acacccaaga acgtgcagac ggaatcgggc
     1861 agaaaatcca cggtttatgc agtcaagatc caggtgcaaa acctcgacca tagactcaaa
     1921 ccggggatgc ctgcagatgt ggagttttct agtaaataga aatcaaaaaa attgtagaag
     1981 cacaagcaat atgcttcgtt tcttgaataa aagtttagta gcaacgaggg actgagcctt
     2041 atcctgaact ggactcagtc ctccttgttt ttaaccttaa ttcagtataa ccaataataa
     2101 tctaaacgtt cagatagttc acgtttggat tgatcgatta aaaaacttta ttaatgaatt
     2161 cactcgcaac ttgctatggg gtgattcact acttttagat acaaaaggag aaaaaaggct
     2221 ttcgccgtat atgttgatga aaaaattttt ttgtatgaat gaagatcgat gattgaaatt
     2281 ggataagttt gaaacctatc aagatgcaat ggcagcaaca aagaaactgg tagacaaatt
     2341 cttacttgag cactacgcac ctggaatgat cagccatggg acccttcgaa ggtaatagag
     2401 atcgggtgtt gatcctcccc ttacaggtga gaggacaggg aaagttaccg agagattgcc
     2461 tgtctgaaca ataaatacta ggcgcataat attgtttctt atcatctcgc agctctcaaa
     2521 acgtaaacta tgataagatt tcgttcaacc gatttaagat aatgagaaaa ggcgctatga
     2581 cttgacatgg aagattgtag gttcctgatt gattggcgtt cctatttggc ctaaatttcc
     2641 attcagcaca ctcttccccc ttaagactct agaggaacga tgttgtaaga acgaagtgca
     2701 caccgttcta tttttcattt gctcttcttt gagaaaacca gatgctacca tcttttccag
     2761 cacgcttccc atttcgtaat ccgtggagat ttgtcaggaa agagttgtaa tttaacccac
     2821 ttaaagcaag attgatttct ccctgaatgg ctctctggca attcaggttg tcgcaaaata
     2881 catacaggtt tccatttgaa gcttcataaa acttcatcca ttttattttt cgggatattt
     2941 gttccttact cacatcccgc tctacttcga cgaatattac ttcacctgtt tttggatcca
     3001 cagcaataat atctgggata taagtttcac cattggataa attaatagct tgtgcccgtc
     3061 ctcggatttt gtaaccttca tctaccagga tttagttggt acaattcagt ttatagcgtt
     3121 atccaccaaa tgcaattata attttgttat tgtttttagt ctcaaattgt attgaacgtt
     3181 aattcccgtt cttacaacat tcgttcagga gacccatatg acccacaaca agcataagtt
     3241 tagtattatt ttaattatct tagtttttat aaccatattg tcggcatgta caacaccaac
     3301 ctcagtaaaa gcaactccgg tccgaataaa gatgtggagt atcggtgttg aagctgatgc
     3361 cactcataac gctttcgcga atgctatttc cacttacaac gccaaacatc aagatgtcca
     3421 aatcgatatt acttatatcg aaagtgagag ttttaagaca caactgcaag ttgcgattgc
     3481 cgcaggcgat ccacctgata ttttccagga ctggggggga ggcgtactaa aaagtttagt
     3541 agatgccggc gttgtacgcg agattgaagc actcaacggg gacgcaagta atatatggca
     3601 accagccgca ttgggtccaa gtacgtttaa tggaaaacat tactctgtcc cagtggacat
     3661 tgtatttgtt tcgttcttct ataacaaaga cttgtttgcc caatatgacg ttgaattacc
     3721 aactacttgg ccaaaattct tagcagcttg taagtctttc agggacgtag gaataatacc
     3781 tgctgtcgtg ggcaataaag agagatggcc tggagcgttg tatacggatt acttaattaa
     3841 ccgtattggt ggacaagaag cgtatgagcg agctacatac gaccaagcaa atggaggttc
     3901 atttaccgat ccaacatttg tgcaagcctg gacgaaaatt caagaagcgt ctaaagcagg
     3961 ctgttttgaa gaaggcgtga atagtggatt atcatctgat gccggaacac ttctgatgac
     4021 tggtagagct gctatgcaat tacaaggcaa ctggatgaca tattattatc gtggaatcga
     4081 caaagtattc acagacaata ctctcgacat cctggcattt ccgtctgttg atggcggtat
     4141 cggaaaccct gctgatttaa tgggtggaac aggtcaagct atggttattt caacgaaagc
     4201 accacctgaa gctgaggctg cactcattga aatgctaggt agtcgacaat tcgccaaaga
     4261 tctagcggat gctggcctca ttccctctat tagaggttcc gaggacttgc tttctaatga
     4321 tccattagcc accaaaatgt tgaaaatgtt ggatacagca ccctctttac atttgctata
     4381 tggtacagat ctacctccaa aattgataga tgaatactgg aattctgctc aacaaatcct
     4441 cgatcttgcg atcacacctg aacaggctgc cgctaacatg caggctgttg cagaaagcct
     4501 aaataacaag taagaaaaag ggcacttcaa tatggaatgg cttcgttttt tttagggaag
     4561 tcattccaag ttatattgaa ataattactg cttatctcaa tttggactgc tgctcttttt
     4621 ctcggtagat tttattgcga tactactgaa taagtaattt atacacggca gttaactatt
     4681 tataataaaa aaatttagtt tgcttttaca ttaagaggga ttaatataat tttattcact
     4741 cgatacatat accttgacgt accttcaaat ttctatgtgg agtaaaatgt tttggacata
     4801 gccaattcgg tcaacataaa acaccgaaag aagtatacag aagccagtgt cacagcagta
     4861 ctttttttat tacctgcaac aattttgttc ttggtatttg tcgtctggcc tatcgttcaa
     4921 tctgcccact atagtgttta ttcgtgggac gggctaggca agccagagca ttttattggt
     4981 ttagaaaatt atgcgcgtct gttaaaagac cctgtttttt ggaaggcttt aggtaataat
     5041 ttttttgtat taatctggtc tttagttaca caaattccat taggaatact acttgccata
     5101 ttgcttactg ggcggataaa aggatcggtt ttttttcgaa cactttatat ttccccaatg
     5161 gtactctcgg gtgtaattgt tggtttattg tggcaatggg tttacaaccc atcatttgga
     5221 ttagctaaca gttttctcac gtggattggt tttgaacaat atacttccgg atggttgggt
     5281 gatgctggaa ttgtgatggt ttgtgtcatg atagttagta cttggcgtga tttaggtttt
     5341 tacataatta tctttattgt ggctatccaa agtatccctg aagagatttg tgcagctgcc
     5401 aaagtagatg gagccaacag ttggcaactt catcgatatg taactttacc gcttataaaa
     5461 aaaacgacac ttactgcttt cgttttaagc ctaattggtt ctttgcaatt tttcgatctt
     5521 acttgggtaa tgactcaagg aggcccgtac cattccagcg aagtggtaac aacctatatg
     5581 ttcaagacag ctttccaatt acgggactgg ggttatgcta ccacccttgc ttttgtactt
     5641 ttttgtatta ctttctttat cgtaattgtt tttctaatat tcaaccaaac acgaaaacgc
     5701 ttcaagtgaa cggagattag tttgtgaaaa aactcctatt tcatctttct atctggtggc
     5761 aatatgcaat tgctctgttg ttttcgtttt ttttcggatt tccgctaatt tggatgtttt
     5821 attcatctct gaaatcaagc cgtgaactta taagtaacat ttgggcattg ccaacccatc
     5881 caactctaaa tgcctacggt ttagttttgg aagttccaga atttaaacta tattatctta
     5941 actcaattgt aataaccgta attagcgtta ttcttctcac ttttctggcc gctatggcag
     6001 cattcgtttt tgcgcgaatt catttttcag gtagtcaggt tttattttac ttttttttgg
     6061 ctggaatgat gattccacca cacgtgactc ttatacctct ctattcgatg ttacgggaaa
     6121 tggggttact caacaaacta atttctctac tatttccata tgtaggattc ggattgccag
     6181 tatcaatata tatattacga gtctattttg aacagatacc tcttgagtta gaagatgctg
     6241 cacgaataga tggcgcctca actgtacgca ttttctggag cattttcttg cccatatctc
     6301 aaccagccct tcttacagtt ataattctaa gcgtgattag cgtctggaat gaatacctgt
     6361 tcgcgttaac acttgtttcg ggtaataatc aatcctatac tctaccattg ggagtacttt
     6421 caatggtcat atctttttca tcattacagt atgatcgagc attaagtgct ctatccctta
     6481 cagctcttcc tgtattattt ttctttttct taatgcatcg tcagattatt aagagtcttg
     6541 ttgcaaaaac attaaattag ttttatccaa ggtaggttaa atggttagaa aacgcattca
     6601 acttgcgcaa aactttctaa agagctcatc attaatatgg aagttaatat cgaaaacatc
     6661 aattgatgac caagatattg tttacgagat aggtgctgga agtggcataa ttaccagtga
     6721 attaattaaa aaagctggta aagtgattgt tatcgaatta gataagaatc taattgataa
     6781 actgattgat atttttggtc aaaatagcaa agtagaaatc catcaaggaa attttttaga
     6841 atatcagtta agttcaccta aatataaagt tttttcaaat atacccttta atatcacttc
     6901 agatattgtc agaaaattac tttattcgtc aaacacccca gaggatgcat atttaatttt
     6961 acaacaagaa gctgcaatga agttctcagg gaatcctacc gaaacagagt tttcaatact
     7021 tgctaagcct tggtttactt ttgaatcggt ttgggatttt cagcgcactg attttttccc
     7081 tgtcccttct gttaatgttt ccttgttaca tattcacaag cgagaaaccc cattggtctc
     7141 ccatgaaaac gcagatttat atagattatt tgtaaaatat ggattcggag cttggaaaaa
     7201 agatttgaaa acagcgtata aagatatttt ttcatatgaa caatggaaac gtttatccaa
     7261 agataatcgt tttccaatta aagccatacc tactcaactt acttttgagc aatggatgaa
     7321 attattcgaa tttttattga aaagtgttcc tccgaataag agaaataatt taaataaact
     7381 ccataaatga cgtaataaga taccattgag aaagtcttta gctcaatgtt gtctcattct
     7441 ggtacctatt cgttattaca gaacatcgtt ccaccttaag actctaggag aacgttgtag
     7501 atagaacggg gtgtacaccg ttctattttt catttgctct tctttgagaa aaccagatgc
     7561 tcccatcctt tcctgcccgt ttcccatttc gtaatccatg tagattcgta aggaaagag
//
LOCUS       DAOPYB010000147         7593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_11682_length_7593_cov_8.998010, whole genome shotgun sequence.
ACCESSION   DAOPYB010000147 DAOPYB010000000
VERSION     DAOPYB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11682_length_7593_cov_8.998010"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKH58_10255"
     CDS             complement(<1..245)
                     /locus_tag="PKH58_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89456.1"
                     /translation="MAFSENNCKSTFITFAAKSTEYGTKKTVQIVCKKGEEGVRMVPG
                     QYLCSDGIEIAVDAGPSFFIPHPFLPDQPGIFFQSVV"
     gene            368..2503
                     /locus_tag="PKH58_10260"
     CDS             368..2503
                     /locus_tag="PKH58_10260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNX89457.1"
                     /translation="MITLEFAIAASCQNVIPLFQPIPIVPKIADQPPMFTCSPELTNY
                     SPFNIDYLFVGNTMTYNSIVGKLSGIITRLSDSTPVENAIITIINDQGQTFITGSDTN
                     GLYSCDSIESGIYLLTVNKEGFNPVADSITIAGNDTLIRDFALTAPTMVINPLEITEI
                     VQIEEFFTRVITVQNTGDGALNWNASIIGPGKDKITIPKGDGNFPCGAAAPSIGRAPL
                     TSHVAGTREIQLKGRSLGYAFEIPGNTFFSFNTDDPSTQNFINTIDYSPSGGTFDAVN
                     TGFMYIIDCNENLFMRVSTCTGDVTIIGSCNPYYGQTWTGITVDKATNKMYGISSDLS
                     ESYIYTINMETGEATVIAATGIPGAIDVAIDENGQMYSFDIVNDEAYKIDKDTGASTL
                     LGSIGFDANYAQGMGWDPEQDIVYLAAYNNDGSGGELRILDRVTGNTNLIGGFPDGEQ
                     IDALAFSGTGLAWLSMNPYHGVIPAGNSQNIAIYLNATILEHGTYTKSITFNSEPNVG
                     TDTVPVTFTVFPEDGPTLTISEVYSHTPLASITVHASNITNMGSFQFTIDYDPTKAIF
                     SGISDWYPGITDVTIGNPSAGKLTFVWAASTGGITINDGDFFTLHLTATDFPPWDYCD
                     ICWSDNPTPREFADWNGNIFTPFYDDGFIYWFVGVPEDKLQPIHIYPNPASDDVQLKV
                     TTPSGTLKYCHSPVKPFTMKIHAMSVKLS"
     gene            2665..4080
                     /locus_tag="PKH58_10265"
     CDS             2665..4080
                     /locus_tag="PKH58_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89458.1"
                     /translation="MKSVYCFLVLLFIVNSVLPQSWSDPINISGIGPNDNPDFTIDKN
                     GVIHCVWSQAFDHYFTKIYYAKSVNNGKTWSTPVPITTNTNLWLDDPHIVADTLGNLY
                     VSYDYNVGGWTKICFVKFNVSDSGWSQQTEIATGVSNKLIIDHNNRLYFFWFAGTEYY
                     RYLDNNVLSDTLSPNSGVTEHYYFDNLTVDINNNIHCVGNRTAGNHSHGAYFKCIYGN
                     WSQYLDLSNHSFFESGVSLNSIGNPSFVWRQIMPDSLPDVRGSYYAELDGDSVQPPVF
                     LAMRTSYPTIAIDNNDQPHIVESQAVDSGYQLFHRYVSGNVWQNEVLEQNKNWYGKNI
                     LISKNSFIYLVYNKVDTTFFSTTGSHNAMIVFRKIEIVPGMIEENQKPELVLYPNPFS
                     EKITLKLLYQNSNPIHLKIFDIYGRTVYEERYEEISPYLNSLVWNGNNNLGAQVKDGC
                     YFLQISQGSRINFRTVIRMNR"
     gene            4115..7132
                     /locus_tag="PKH58_10270"
     CDS             4115..7132
                     /locus_tag="PKH58_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89459.1"
                     /translation="MKNLQVFLIIMIISVLTVTSMGQNKYAILVGGPIDKSHWRPSQY
                     NGGWFPQEYQDFFWTDIFLMHQLLINKLDYPAENVKIIFDNGQDYSPPDNPGPYPTRY
                     KKEHTYDPDITAPDHLACTYTNVKTMLQNINTNENDFVFIYIFTHGRYPNGDQPVPYG
                     ILCPQQLHNDYLYDDSDYMYANEFADLVTNITAQKKVIWMQPCYSGGFKNYFNNHLDR
                     SPFIVNYACGALEEAFQSDDMIYCPGYGLQQINENEPPFFYLPPYYSGEWGIQCHHGE
                     FSYHLYSALNGETPTIGETEYFNMPINIPLSSADNNPNDGIISVKEDYDWANQYQSWK
                     YHDVGEHLDYQESIVGLSNKTSLMYPNLVSSITDLHVGLTNTGIYGVTSDVIVSGANN
                     SLSFGANSKIYLLNTAKIRVQGGGSLTIGDGVKIYGNGQNSVRIENGTINLGQNVLFS
                     SRNDDQVFDGLHLSNYATPTQINHATFHRAHLWNESSSLTINYTNFDHVATSEEDIYG
                     VYSTAGNVTISHSTFDVASVYLINSNSQQNPNWLAFVQHCEFHGGGIYLLGYRKLKID
                     YNEIEHQNKIAIETSYCGNGTSLNQSITHNEIYNSHTGLFLNNTFAEINNNNIHNNYH
                     GIYLGNHSQISTLKGTSSLTQKIKDCVLEELYIEHGCFPVLFKYNQIIDEDNFGGTLD
                     PLLCYVSLPHVYEYYDISSNCWGNNFNPNMDLTVMGEYANVSFLIYPTWCPGNSVPTD
                     PPGDMYANAIIKYDSGNYTEAKSIFKMIIESYPKTFQAQAAMKELFQIEEMSDNDYLT
                     LQQYYLTNDSIVADSNLTLSGMYLANRCNEKIENWPDEIAWFENKIMNPESEADSIFA
                     ILDLSHVYLLMENSPTKAAYLGTLQQYKPKSLQQFIPYQNYLISLLPGDSKYEQLLKR
                     ISNLNDGELLQNNPNPVIQSTDIYYKINSSGDILITITDIFHRIVKTIHENNISTGTH
                     KVSVNLSDLNPGLYFYTLTINGKISDIKKLTML"
     gene            complement(7322..>7593)
                     /locus_tag="PKH58_10275"
     CDS             complement(7322..>7593)
                     /locus_tag="PKH58_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89460.1"
                     /translation="FHKMDETIRGHVFCSFLALVLRKELDKRLEIKGKNYEWNDIKRD
                     LTNLREVTLSEEHTSLAIRTACQGICADVFKSVGVALPPAIKSLS"
BASE COUNT         2379 a         1515 c         1364 g         2335 t
ORIGIN      
        1 taaacgacag actggaaaaa tatcccaggt tgatcaggta aaaaaggatg cgggataaaa
       61 aaagaaggac cagcatcaac agcaatttca ataccatcac tgcatagata ttgccccgga
      121 accatcctga ccccttcttc ccctttttta cagacgatct ggaccgtctt ctttgtgcca
      181 tactcagttg atttggctgc aaaggtaatg aaagtggatt tacaattatt ttcagaaaaa
      241 gccatttgtt attggattct ttctggcatc attatatatt tgtaataata tatttagcca
      301 aacctaataa taactatatt cattcatcat gaaatatata ttcaattttc tgaaattctc
      361 ctaggtattg ataactctcg aattcgctat tgctgcttct tgtcaaaatg taataccctt
      421 atttcaacct attcctatcg tccctaaaat agcagaccaa cccccgatgt ttacctgttc
      481 accggaattg actaattaca gtccttttaa catcgattat ttgttcgttg gtaataccat
      541 gacttacaac agtatagtcg gtaagctttc tggaattatc accagattat ccgacagcac
      601 tcctgtcgaa aacgcgatta tcaccatcat caacgatcag ggtcaaactt ttataaccgg
      661 ttcagacacc aatggcctct attcctgtga tagcattgaa tcgggtatct acctccttac
      721 agttaacaaa gagggcttca atccagttgc cgacagtatc accattgcgg gtaatgacac
      781 gttaatccgg gattttgcac taaccgctcc gactatggtt atcaatccac tggaaatcac
      841 tgaaatagtg caaattgaag aattttttac ccgcgttatc acggtgcaga atacaggaga
      901 cggggccctg aattggaatg catcaataat cggacctggc aaagacaaaa ttactatccc
      961 taaaggcgat ggtaactttc catgtggcgc tgcagcccct tcaatcggca gggccccgct
     1021 tacaagccat gtagctggta cccgggaaat ccagctaaaa ggcagatctc ttggttatgc
     1081 cttcgaaatc cccggaaaca ccttcttctc attcaacacg gatgatcctt ctactcagaa
     1141 ttttatcaac actatcgact attcgccgtc tggtggaact ttcgatgcag tgaataccgg
     1201 ttttatgtat atcatcgatt gcaatgaaaa cctatttatg agagtttcca cttgtaccgg
     1261 cgatgttact atcattggct cgtgcaatcc ttactacgga caaacctgga ccggcattac
     1321 agttgataaa gcaacaaata aaatgtacgg aatctcaagt gatctgtcag aatcttacat
     1381 ctataccatc aatatggaaa ccggcgaagc tactgtcatt gcagcaaccg gtatccccgg
     1441 tgccatcgat gtcgctattg acgagaatgg gcagatgtac agctttgata ttgtcaacga
     1501 tgaagcttac aagatcgaca aagacacagg cgcttccacc ttactgggct ccattggctt
     1561 tgacgccaac tatgcccagg gcatgggctg ggatcctgaa caggatattg tttatcttgc
     1621 tgcttacaac aacgacggtt caggtggtga actccgtatc ctcgacagag taaccggaaa
     1681 tactaactta ataggtggat ttcccgatgg cgaacaaatc gatgcccttg ccttcagtgg
     1741 cacaggttta gcctggctgt caatgaatcc atatcatggg gttattcctg caggtaattc
     1801 acagaatata gctatatatc ttaacgccac tatacttgaa catggaacct ataccaaatc
     1861 cattaccttt aatagcgaac ccaatgtagg tactgatact gtcccagtaa cttttactgt
     1921 ctttcccgaa gacggcccaa ccctcacgat ttcggaggtc tattctcata cccccctggc
     1981 ttctatcacg gttcatgctt ctaacattac caacatgggc tcttttcagt tcacgatcga
     2041 ttatgatcca acgaaagcga tcttttcagg gatttccgac tggtatcccg ggattaccga
     2101 tgtcaccatc ggtaatccat cggcaggcaa actcactttt gtatgggcgg cttcgaccgg
     2161 aggcatcacc atcaacgacg gtgacttctt cacccttcat ctcacagcca cagattttcc
     2221 cccttgggac tactgcgaca tctgttggag tgataacccg accccacgcg aatttgcgga
     2281 ttggaatggt aatatcttta ccccatttta tgacgatggc ttcatttact ggtttgtggg
     2341 agtacctgaa gacaagctgc agcccatcca tatttacccc aacccggctt ctgatgacgt
     2401 acaattaaaa gtgactaccc catcaggaac attgaaatat tgtcattcac cggtcaaacc
     2461 gtttacaatg aaaatccacg caatgtcagt gaagttaagt tgagcgttgc ctcctggcct
     2521 tccggcgtat tttttgtgaa gatcatcacg cagcaaggtg taaccacctc caagattgtc
     2581 gtggaacatt gatctggcga gcccccacaa gttacatata aaatggagtc cactttaacc
     2641 ctaatttgtt tatatctttg aaaaatgaaa tcagtatatt gttttctagt cttgctgttt
     2701 attgttaata gtgtgctccc tcaatcctgg tcagatccga taaatataag cggaataggc
     2761 cccaatgaca atcctgattt tacaatcgat aaaaatggag tgatccattg tgtttggtca
     2821 caggcattcg atcactattt cacaaagatc tattatgcaa aatcagtgaa taatggaaaa
     2881 acctggtcaa cgcctgtgcc aatcacaaca aatacgaatt tatggcttga tgacccccat
     2941 atcgttgcag atactttggg aaatttatat gtaagttacg actataatgt tggtggctgg
     3001 acaaaaattt gttttgtgaa atttaatgta agtgattcag gatggagcca acaaacagag
     3061 attgctactg gtgtctcaaa caaacttatt attgaccaca acaacaggct gtatttcttt
     3121 tggtttgcag gaacggaata ttataggtat ttggataaca atgttcttag tgacacgctt
     3181 tcacctaatt caggtgtgac ggagcactat tattttgaca atttaactgt tgatataaat
     3241 aataacatac attgtgtagg taatcgtaca gccggaaacc attcgcatgg cgcctacttt
     3301 aaatgtattt atggaaattg gagtcaatat ttagatttaa gcaatcattc attcttcgaa
     3361 tctggggtta gcttaaacag cattgggaat ccctcttttg tctggcgaca aataatgcct
     3421 gattctttgc ctgatgtcag aggatcttat tatgcagaac ttgacgggga ttcagttcaa
     3481 ccccctgttt ttttagccat gagaacatct taccctacaa ttgcaatcga taacaatgat
     3541 caaccgcata ttgtagaaag tcaagcggtt gatagtggtt atcaactatt tcatcgatat
     3601 gtttccggga atgtttggca aaatgaagtg ttggaacaaa ataaaaattg gtatggaaag
     3661 aatattctaa tatccaagaa ttcttttata tacttggttt ataacaaagt tgatacaaca
     3721 tttttctcca caacaggtag ccataatgca atgattgttt tcagaaaaat agaaatagta
     3781 ccaggtatga ttgaggaaaa tcaaaaacca gaattggttt tatatccaaa tccgttttca
     3841 gaaaaaatca ccttaaaatt gctataccaa aactcaaatc caattcactt aaaaatcttt
     3901 gatatctatg gtcgtaccgt ttatgaggaa aggtatgaag aaatatctcc atatctcaac
     3961 agccttgtct ggaatggaaa taataacctt ggtgcccaag tcaaagatgg ttgctatttt
     4021 cttcaaatat cacaaggctc aaggatcaat tttagaactg taattcgcat gaacagatag
     4081 ttttacgcgt tttaaactct aaaatgatat gggtatgaaa aatctccaag tatttctgat
     4141 tattatgata attagtgtcc ttactgttac ttcaatgggc caaaataagt atgcgatact
     4201 tgtcggtggt cctattgaca aatcacattg gcgtcctagc caatataatg gcggatggtt
     4261 tccgcaagag tatcaagatt ttttctggac ggatattttt ctgatgcacc aactattaat
     4321 aaataaactc gattatcctg cagaaaacgt taaaatcatt tttgataatg gtcaagatta
     4381 ctcaccaccc gataatcccg gtccttatcc tacaagatat aaaaaagaac acacctatga
     4441 tcctgatatt acagcaccag atcacctagc ttgtacatat actaatgtta aaacaatgct
     4501 acaaaatata aataccaatg aaaatgattt tgtattcatt tatatattta cccatgggcg
     4561 ctatcctaat ggggaccaac ctgttccgta tggaatttta tgtccacaac aacttcataa
     4621 tgactattta tacgatgata gtgattatat gtatgctaat gaattcgctg atttggtgac
     4681 taatataaca gctcaaaaaa aggtaatttg gatgcaaccc tgttattcag gtggatttaa
     4741 aaattatttc aataatcact tggatagaag tccgtttatt gttaactatg cttgtggtgc
     4801 tttggaggag gcattccagt cagatgacat gatttattgt cctggttatg ggctacaaca
     4861 aatcaatgaa aatgaaccac ctttttttta tctaccacca tattatagtg gagaatgggg
     4921 aatacaatgt caccacggtg agttcagtta tcatttatat tctgcgttaa atggtgaaac
     4981 tcctacgatt ggtgagactg aatattttaa tatgcctata aatattccac tctcatcggc
     5041 tgataataat ccaaatgatg gcattatttc tgtaaaagag gattatgatt gggcaaatca
     5101 atatcaatca tggaaatatc atgatgttgg ggagcatctt gattatcagg aatccatagt
     5161 aggtttatct aataaaactt cattgatgta tccaaacctt gtttcctcaa tcaccgattt
     5221 acacgtaggt ttaacaaata ctggaattta tggtgtcact tctgatgtta tagtttcagg
     5281 tgccaataat tctttatctt tcggcgcaaa ctcaaaaatt tatttattga atactgcaaa
     5341 aattagagtg caaggtggtg gttctcttac tattggggat ggtgtcaaga tttatgggaa
     5401 cggtcaaaac agtgtccgaa ttgaaaacgg aacaataaat ttaggacaaa acgtgctatt
     5461 ctcaagtaga aatgatgatc aagtttttga tggcttgcac ctgtccaact atgccacccc
     5521 cacacaaatc aatcatgcaa cttttcatcg tgctcatctt tggaacgaaa gttcaagcct
     5581 tactattaat tatactaatt ttgaccatgt agcaacttcc gaagaagata tttatggagt
     5641 ttactcgacc gcaggaaatg taacaatatc tcattcgaca tttgatgtag cttcagttta
     5701 cttgatcaac tcaaattcac aacagaatcc taactggctg gcatttgtac aacattgtga
     5761 atttcatggg ggaggaattt acctcctcgg ttaccgcaaa ttaaaaattg attacaatga
     5821 gattgaacac caaaataaaa ttgcaataga aacaagttat tgtggtaatg gtacttcatt
     5881 gaatcagtca ataacacata atgagatata taatagtcat accggcttat tccttaacaa
     5941 tacttttgcg gaaatcaata ataataatat ccataataat tatcatggaa tctatttggg
     6001 caaccattcc cagatttcaa ctttaaaagg cacttcatct ttaactcaaa aaataaaaga
     6061 ttgtgtttta gaggagttat acatcgagca tggatgcttt ccggtacttt ttaaatataa
     6121 tcaaatcatt gatgaagata atttcggtgg gacgttagat ccattattat gttatgtaag
     6181 tctgccacat gtgtatgaat attacgatat ttcatcgaat tgttggggta ataattttaa
     6241 cccgaacatg gatttgacag taatgggtga gtacgcgaat gtatcctttc ttatttaccc
     6301 tacatggtgt cccgggaata gtgtgccaac agacccccct ggcgacatgt atgcaaatgc
     6361 aattattaaa tatgattcag gaaactacac tgaagcaaag tctatattca agatgattat
     6421 tgagtcatac ccaaaaactt ttcaagcaca ggcagcaatg aaagaattat tccaaattga
     6481 agagatgtca gataatgatt atctgacttt gcaacaatat tatttaacca atgattcaat
     6541 tgttgcagat agcaacttga ccttatctgg catgtatctt gcaaacagat gcaatgagaa
     6601 aattgaaaat tggccggatg aaattgcttg gtttgaaaat aaaataatga atcccgaatc
     6661 agaagcagac agtatttttg cgattttaga cctttctcat gtataccttc ttatggaaaa
     6721 tagtccaaca aaagctgctt atcttggtac attgcaacaa tacaagccga aatccttaca
     6781 acaatttatc ccttatcaaa attatttgat ctcgctactt ccaggggatt caaaatatga
     6841 gcaattattg aaaagaatta gtaatttaaa tgatggtgaa ctactgcaga acaatcctaa
     6901 tccagtgatt caaagtactg atatttatta taagattaac tcatctggtg atatccttat
     6961 taccattaca gatattttcc atcgtattgt caaaacaatt catgaaaaca atatttccac
     7021 tggtactcat aaagtgagtg tcaatctttc tgatttgaat ccgggattgt atttttatac
     7081 attgacaatt aatggaaaga tttcagatat taaaaagtta actatgttgt aaacctagcc
     7141 ttttcaatta aaccactctg aattgaaaat cacatcattg atgacctttt caaggtcaat
     7201 gccccaactc atcttctaca gtagtatttc gtaacatatt ttcagacagc tcattataga
     7261 cactcaaaaa gttcttacca ccacaaatgg taattatgac atataacaac aagatggttt
     7321 cttagctcaa tgattttatg gccggtggca acgcaacccc aacagatttg aaaacatcgg
     7381 cacaaattcc ctggcatgct gttctgattg caagacttgt gtgttcttct gaaagggtta
     7441 cctccctcag atttgtaagg tcacgtttaa tgtcgttcca ctcatagttt ttccctttga
     7501 tttccaaccg cttgtcaagt tcttttctca ataccagcgc cagaaagctg caaaagacgt
     7561 ggccgcggat ggtttcatcc atcttgtgga aga
//
LOCUS       DAOPYB010000148         7582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_11712_length_7582_cov_10.536734, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000148 DAOPYB010000000
VERSION     DAOPYB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11712_length_7582_cov_10.536734"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            499..1500
                     /locus_tag="PKH58_10280"
     CDS             499..1500
                     /locus_tag="PKH58_10280"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNX89461.1"
                     /translation="MKKKNLSELKVVITLLLSVIALTTFAQNLTLTGTVTDINRGPII
                     GATVVVQGSVNQGTVTNFEGKFVLTNVPSNGTIHVSYVGMQPQDLSVGGRTNIDVILQ
                     EDSKVLGEVVVTALGIKRESKALGYSISTISAKDIVRAGAPNFATSLYGKATGVRIQA
                     APGGSTSAVSINVRGLSSITGTNQPLVIVNGVPIRNGDANNSDYWSDQRINSNGLIDI
                     NSEDIESISILKGASASALYGSEAANGVVMITTKSGRTSSRLGVDFNANLSGDMVAYM
                     PEYQTVFGPGVRVGGRGSYEKSTGGFWERTYNGQTYKSIRTATATYGPKYDGSDVLY"
     gene            1382..3820
                     /locus_tag="PKH58_10285"
     CDS             1382..3820
                     /locus_tag="PKH58_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX89462.1"
                     /translation="MRKVQEDFGKGPIMVRPTNLSELQPLHTVQSMTAAMYFTDGTVH
                     KYEPVSSSPWNEIFRTGYNQTYNVGITQGTDKGNMRFSYTYVNNLPTQYNSTYNKNNF
                     SLTGSYSLNDNLKLQYSANYIIQNIKNRPYRISRLTNNFSGMVNAYDDVAYIRENSVT
                     SLGYMNVTTGENTLTPKESFAYPLAAGSLVSEYFWNIYGKEQLENNNRLIASVTPSWK
                     IIEGLTLQGRISTDYTTEKIENKNKTEKPLVFGSYTGYYGLSNRRYEIFYGDLLLMFN
                     RNLTDKLGLVANVGWQGRTEKSFNTSVGTNGGLSVENWFHLNASKNTPSSGMYESSYL
                     KQAFLGTVSFSWDNYLYVEATGRQEKSSTLAQGNNSFFYPSVNSSFIYTDAFKNALPN
                     WYDYGKLRVSYGKVGNAPELYRAVQAFNQSSASGYIYNTVPSNLGNDKIRPEQKYEFE
                     IGWENKFFDNRLGFEATYYNNKIKDQILQTTTPNTAGGSSILLNIGELHNHGFEFSAY
                     GTPFKTKDWNWDLRGNIAFNRNTVTKLMEGIDVLVHQDIDGGAVRIESHVGEPMGDLY
                     AYAPLQDSKGNFVVGSDGFYKLTPDRVKVGNAMPKAIGGFGSTLRYKNISFDATIDFR
                     IGGAVLNTPYEYLMGRGSLVESMNYRDTDHGGLKYYFANNDGGTMPVTLGNGTAPSGA
                     IVYDNGLILPGVKEDGSVNDIMLESDKWYNWTYNWGVGDPTYYSHAIFDNSYVKVREL
                     ALSYMLPRNVISKIGCNNLSLSAFARNPFYIYKNLPIFDAEATDGTSWISQAQIGGST
                     ATTRTFGVSLRATF"
     gene            3847..5403
                     /locus_tag="PKH58_10290"
     CDS             3847..5403
                     /locus_tag="PKH58_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX89463.1"
                     /translation="MKNILSKLFIFAMIISMASCSDEAFTSKYADPGKTSTASCEKLM
                     TGVFYSGREYTFNSYWRIFTWDYSAISRYAQTLGVLNGDGRYQYSDSYASDRWNNFYN
                     VLSQFRVLENEYNKLSDADKANYEVFVLLAKVYMYDHLIQITNCWGDVPYSKAGYLSI
                     TGDVATSNPAYDTAESIYSAVMSDLKAINTKLAGMTSLSPLTTSYLKAQDFINGGNLL
                     SWRKYANSLRLRVATTVADNGTLTADARAAIREMLTDPSTYPMVDSNSENIKVIPDTD
                     GFNYGQQYQDGWETWIGELNRASKAMVDALQGDARMDILFDKNKGGNYVGVDTHTDFA
                     TQSTLFESGTHYSAYDSATFSRNKKLEGIIISAAEVALHKSNAFSKGYATGDAKTAFV
                     KGMVLSTEMYYGINAQATYRTPTAAPATNAVQAFATAKWDAASNKDAEIATQTWLNFG
                     FLNSTQAWTTVRRTGYPVLYFPTDNSSATCPNVPVRLRYPPSERNSNTANYTPFKNED
                     NYTTKMFWAK"
     gene            5502..6881
                     /locus_tag="PKH58_10295"
     CDS             5502..6881
                     /locus_tag="PKH58_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX89464.1"
                     /translation="MKIKYVLLVAAAVFSTLVKSQESFVHGVSDEYVWPTDEKVLTKL
                     HKWQDLKFGLIIHWGIYSVPGIVESWSICSEDWITRDTTRTYDEYKNWYWGLAKQFAP
                     AEYNPQLWADAAKNAGMKYVVFTTKHHDGFNMYDTKYSDFSITKGPFRDNPKADVAKF
                     VFNAFKEKGFMIGAYYSKPDWHCPDYWWPYRATPDRNQNYSIEKEPARWNRYKTFAYN
                     QIEELMNNYGSIDILWLDGGWVNSERRKAFIDMPKIAEMSRKAQPGIIIVDRTIQGEF
                     ENYQTPEQQIPATQLPNPWESCMTLGTDWGWTPNAKFKSTTFVIGKLMEIVAKGGNLL
                     LGAGPDSKGVFPNEVYSRFAEIGKWLKVNGEAIYETTITPDYHENNTWFTANKDGKKM
                     YALYALPDGDSLPETIEWQINLPKKGTKVILLQTGKSVSYTIKNDKIVIKLPKGLRNE
                     PLAFSFQVK"
     gene            7006..7290
                     /locus_tag="PKH58_10300"
     CDS             7006..7290
                     /locus_tag="PKH58_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNX89465.1"
                     /translation="MKLRLYYVYILTTKSNTVLYTGVTNDIKRRVFEHKFGFNEGFTK
                     KYHVHKLVYFEVFDFVELAIVHEKQIKGYSREKKIKLIEALNPDWKEMEV"
BASE COUNT         2510 a         1353 c         1519 g         2200 t
ORIGIN      
        1 attcaaaatc ccccgaaaag ccgatatgct cataaaagtg tatcggcttt ttttattgaa
       61 tagaaatcat acacctgcga agtattcaaa caaaatgatg atttctaaaa aaacgaatca
      121 ttagtttcga aaaataatca tcatttgaat attctctttt ctattttttt tgactttatc
      181 cccaccttaa gacacaaagt aacttttttt aattgaatat tcttcatttt attcttaaaa
      241 ttaaaattta ttctctttct ttgtaaaaat aatttactca atttttcaat tcatgcaatt
      301 actcaatcaa aaccacaaaa aaatcatttt gtaacttgtt ttgattaatt tttccatgat
      361 caccattcca tcttgcatta ttcgttaaat taattcatta ttttgaatta ttgacagata
      421 gtccgatttt ctcgattacg aacttattgt tattttcgaa atataatttt catattatta
      481 acattaattt acgagagaat gaaaaaaaag aacctttcag aactgaaggt tgtaattaca
      541 ctattattaa gtgtaattgc ccttacaact ttcgcacaaa atctcactct tacaggaacg
      601 gtaactgata ttaacagagg acctattatt ggcgcaacag tagtagttca gggatctgtc
      661 aatcagggta ctgtgacaaa ttttgaagga aaatttgttt taacaaatgt accttcaaac
      721 gggaccattc atgttagtta tgtcggtatg cagcctcaag acctttcggt gggtggcaga
      781 accaatatcg acgtaatcct gcaggaagac agcaaagttc ttggagaagt ggtggttacc
      841 gctttaggca ttaaaagaga atctaaagct ttaggttact ccatctccac tatttctgcc
      901 aaagatattg taagagccgg agctccgaac tttgcaacat ctttgtatgg aaaagctacc
      961 ggagtccgaa ttcaggccgc acccggagga agcacttcag ctgtttctat taatgtacgt
     1021 ggcttaagtt ctattactgg taccaatcaa cctttagtaa ttgtaaatgg tgtgccaata
     1081 aggaatggag atgcaaacaa tagtgattat tggtcagatc aacgtataaa cagcaatggt
     1141 ttgattgaca ttaactctga agatattgaa agtatatcta tactaaaagg ggcatcagca
     1201 tcggctctat atggttctga agctgccaac ggtgtggtaa tgattaccac gaaaagtgga
     1261 agaacaagtt ccaggcttgg tgttgatttc aatgctaacc ttagtggtga catggtagcc
     1321 tatatgcctg aatatcagac agtatttggc ccgggggtca gagttggggg acgtggtagt
     1381 tatgagaaaa gtacaggagg attttgggaa aggacctata atggtcagac ctacaaatct
     1441 atcagaactg caaccgctac atacggtcca aagtatgacg gcagcgatgt actttactga
     1501 tggaacggta cataaatacg aaccggtaag ttcaagtccc tggaatgaaa ttttcagaac
     1561 cggatacaac caaacatata atgtaggtat cacacaaggc accgataaag gcaatatgcg
     1621 tttttcatat acgtatgtaa acaatttacc gacacaatac aatagcacat ataataagaa
     1681 taacttcagt ttaaccggct cttattcatt gaatgacaac ctaaaattgc aatattcagc
     1741 caattatatt atccagaata taaagaaccg tccttaccgc atcagtcggt taaccaacaa
     1801 tttttcggga atggttaatg cttatgatga tgttgcatac atcagagaaa actctgtaac
     1861 aagtttagga tatatgaatg ttactacagg cgagaacacc cttaccccta aagaatcatt
     1921 cgcttatccg ttggctgcag gttcgcttgt ctcagaatat ttctggaata tttatggtaa
     1981 agaacaattg gaaaacaata accgtttaat cgcaagtgtt actccaagct ggaaaatcat
     2041 tgaagggtta acacttcagg gacgtatttc aaccgactac accacagaaa aaatagaaaa
     2101 taaaaacaag acagaaaaac ctttagtatt cggatcttat accggatatt acggtttatc
     2161 taacagaaga tatgaaattt tctatggtga tttactcctc atgttcaaca gaaatctgac
     2221 tgataaatta ggtttggttg ctaacgtagg atggcaggga cgtaccgaaa agtcatttaa
     2281 tacttctgta gggacaaatg gtggattatc ggttgaaaat tggtttcatc ttaatgccag
     2341 taaaaacacc ccttcctctg gcatgtatga gagctcttat ttaaaacaag cgtttttagg
     2401 aacagttagt ttttcatggg ataattattt atatgtagaa gctaccggta gacaagaaaa
     2461 gagttcaaca ttagctcagg gaaataattc tttcttctat ccatccgtaa actccagctt
     2521 tatctacaca gacgccttca agaatgcatt acccaattgg tacgattatg gaaaactgcg
     2581 tgtatcatac ggtaaagtag gtaatgcacc ggaattatac agagcagtac aagcttttaa
     2641 tcaatcatct gcttcgggat atatatataa tactgttcca tcaaatttag gaaatgacaa
     2701 aattcgtcct gaacaaaaat atgaatttga aattggatgg gagaataaat tttttgacaa
     2761 cagacttgga tttgaagcta cttattacaa caacaaaatt aaagatcaaa tacttcagac
     2821 cacaactcca aacacggcag gtggctcatc tattctgttg aatataggtg aactccacaa
     2881 ccatggattt gaattctcag cttatggcac cccattcaaa acaaaagact ggaattggga
     2941 tttgagagga aatatcgcat tcaacagaaa tacagttacc aaactgatgg aaggtattga
     3001 cgttttggtt catcaggata ttgatggtgg agctgtacga attgaatcgc atgttggcga
     3061 accaatggga gatctttatg cttatgcacc gcttcaagac tcaaaaggaa attttgttgt
     3121 tggttctgac ggattttaca agctaactcc ggacagggtc aaagttggta atgcaatgcc
     3181 taaagcaatc ggtggttttg gttctacatt gagatataaa aacatatctt ttgatgccac
     3241 aattgacttc cgcatcggtg gtgcagtatt aaatacacct tatgaatacc tgatgggacg
     3301 tggatcattg gtagaatcca tgaattatcg tgataccgat catggtggtc tgaaatacta
     3361 tttcgcaaat aatgacggag gtacaatgcc tgttactctc ggaaacggca cagcgccatc
     3421 aggagctatt gtttatgata acggacttat tctgcccggt gtgaaggaag atggttccgt
     3481 aaacgacatc atgcttgaat cagacaaatg gtataactgg acttataact ggggagttgg
     3541 tgatccgacc tactattcac atgccatttt cgacaatagc tatgtaaaag ttcgtgaatt
     3601 agctttaagc tatatgttac caagaaatgt tatctcgaaa attggctgta acaatctttc
     3661 attaagtgca tttgcgagaa atccattcta tatttataaa aatcttccaa tatttgatgc
     3721 agaagcaaca gatggaacat cctggatatc tcaggctcaa attggaggtt ctacagctac
     3781 taccagaaca ttcggtgtat cacttagggc aactttttaa tcatcaaaca tttaaatgaa
     3841 aatataatga aaaatatatt atcaaaacta ttcatttttg ccatgattat atcaatggca
     3901 tcttgcagcg atgaagcttt taccagcaag tacgcagacc cgggtaaaac cagtactgct
     3961 tcctgcgaga agttaatgac tggtgtattt tacagcggaa gggaatatac attcaattca
     4021 tactggagaa tattcacatg ggattattca gctataagtc gctatgctca gacattaggt
     4081 gtactgaacg gagatggtcg ttaccaatac tccgacagct atgcttctga cagatggaac
     4141 aatttttaca atgttctttc gcaattcaga gttctggaaa acgaatataa taagctaagt
     4201 gatgctgaca aagctaatta tgaagttttc gtattgcttg caaaagttta catgtacgat
     4261 caccttatcc agataacaaa ctgctggggc gatgtacctt attctaaagc aggttattta
     4321 tccattacgg gtgatgtagc aacatcaaat ccggcttacg atacagctga atctatttat
     4381 tcggctgtga tgtcagacct gaaagccatt aatacaaaat tagcaggaat gacatcgtta
     4441 tcaccgttga caacaagcta tctgaaagcc caggatttca tcaacggtgg caatttatta
     4501 tcatggagaa aatatgccaa ttcattgcgt ttacgtgttg ccactactgt tgccgataat
     4561 ggtactttaa ccgctgatgc aagagcagct atccgtgaaa tgcttacgga cccttcaact
     4621 tatccaatgg tagattcaaa ttcagagaac ataaaggtta ttccggatac cgatggattt
     4681 aattatggtc aacaatatca ggatggatgg gagacatgga taggagaact taaccgtgct
     4741 tcaaaggcga tggttgatgc gctgcaggga gatgctcgta tggatatcct gtttgacaag
     4801 aacaaaggtg gaaattatgt tggtgtagat actcatactg attttgcaac tcaaagcacg
     4861 ctgttcgaaa gtggcacaca ttatagcgcc tatgattctg ccacgttcag cagaaacaaa
     4921 aagctggagg gaataatcat atcagcagct gaagttgctc tgcataaatc aaatgcgttt
     4981 agtaaaggat atgcaacagg tgatgctaaa actgcctttg tcaaaggcat ggtgctttcc
     5041 actgaaatgt attatggcat caatgctcag gcaacttatc gcactcctac agcagctccg
     5101 gcaaccaatg ctgttcaggc ttttgcaacc gctaaatggg acgcagcttc caataaagat
     5161 gcagaaattg ccactcagac atggctcaac ttcggattcc tgaattcaac tcaggcatgg
     5221 acaacagtaa gaagaaccgg ttatcctgta ttgtacttcc caaccgataa ttcatcggca
     5281 acatgcccga atgtaccggt tcggttacgg tatccgccaa gtgaaaggaa ttccaacacg
     5341 gcaaattata cacctttcaa aaacgaagat aattatacaa ctaaaatgtt ttgggctaaa
     5401 tagaactaac atcattttag agaaaggtta aaacgaatga ggaatcgttg tgaaagtagt
     5461 atacagacat aacgattcct tcattttcaa taattacaac aatgaaaata aaatacgtgt
     5521 tattggtagc ggccgcagtt ttttcaactt tggttaagtc gcaggaaagt tttgtacatg
     5581 gtgtatcgga tgaatatgtc tggcccaccg atgaaaaagt gctaacgaaa ttacacaagt
     5641 ggcaggattt gaaatttgga ttgattattc actggggtat ttattctgtt cccggaattg
     5701 tagaatcgtg gtcaatttgc tcagaagact ggattacccg cgacactaca agaacttatg
     5761 atgaatataa aaactggtat tgggggttgg ctaaacaatt tgcacctgct gagtataatc
     5821 cacaattatg ggccgatgcg gcaaaaaacg ccggaatgaa atatgtggta ttcaccacaa
     5881 aacatcatga cggatttaat atgtacgata caaagtattc tgatttttct atcaccaaag
     5941 gtccattcag ggataatcct aaagcggatg tagcaaaatt cgtatttaat gcctttaagg
     6001 aaaaagggtt tatgattggt gcctattact ccaaacccga ctggcattgt ccggattatt
     6061 ggtggcctta tcgggcaact ccggatcgga atcaaaatta cagtatcgaa aaagaacctg
     6121 cgaggtggaa tagatataaa acattcgcat acaatcagat tgaagaatta atgaataatt
     6181 atggctctat tgatattctt tggctggatg gcggatgggt aaacagtgaa cgcaggaaag
     6241 cgtttatcga tatgcctaaa atagcagaga tgtcacgtaa agctcaaccg ggaataatta
     6301 ttgtggatag aacaatacag ggtgaatttg aaaactatca aactccggaa cagcaaattc
     6361 ccgccacaca gcttccaaat ccatgggaaa gctgcatgac gctcggcacg gactggggat
     6421 ggacacccaa tgcaaaattt aaatcaacca cctttgtaat tggaaaatta atggaaattg
     6481 tggcgaaggg tggaaattta ttgttgggcg caggtcccga ttccaaagga gtttttccga
     6541 atgaggtata ttcacgcttt gctgaaattg gcaaatggct taaggtaaac ggagaagcta
     6601 tttacgaaac aaccattact cccgattatc atgagaataa tacctggttt acagcaaata
     6661 aagatggaaa aaaaatgtat gcactttacg ctttaccgga tggagatagt ctacctgaga
     6721 cgatagaatg gcaaatcaac cttccaaaaa aaggaactaa agtgattttg ttacaaactg
     6781 gaaaatctgt aagttacaca attaaaaatg ataaaatagt tatcaaactt ccaaaaggat
     6841 taagaaacga acccctggct ttttcatttc aggtaaagta gaggaagttg cgaaccgttg
     6901 ccaagattat agtgatgtta caatatcgtg tcgcgcaacg gaattggcta acggctattt
     6961 gtcatcccga tgcgcagcga gggatctaaa agtaactgat taactatgaa attacgcctt
     7021 tattatgttt atattcttac tacgaaaagc aatacagtcc tctataccgg tgtaacgaat
     7081 gatataaaac gtagagtttt tgaacacaaa ttcggattca atgaagggtt tacaaaaaaa
     7141 tatcatgttc acaaattggt ttatttcgaa gtgtttgatt ttgtagaatt ggctattgtc
     7201 catgaaaagc aaataaaggg ttattccaga gagaaaaaaa ttaaactaat tgaggcattg
     7261 aatcctgatt ggaaggaaat ggaagtttaa tgattagatt cctcacttcg tttttatggc
     7321 gtaggccaaa aaaaacttgt tcggaatgac agaatcattc tttatcaatt acccgtcgat
     7381 acggtttgtg agaatgatga ggtttaacac tacgggttct tggggagttt atgtgtcgct
     7441 gccacactta tgatgatgaa gtaaacaaca gtcgggcagc ggaatttact aaaacggttg
     7501 tctgtcatcc cgaatggaac gcagtgaagt gagggatcta aaaaagtctt taattatgtc
     7561 attgatagtg tcccgaattc tc
//
LOCUS       DAOPYB010000149         7473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_11954_length_7473_cov_10.560933, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000149 DAOPYB010000000
VERSION     DAOPYB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11954_length_7473_cov_10.560933"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PKH58_10305"
     CDS             <1..980
                     /locus_tag="PKH58_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HNX89466.1"
                     /translation="KMRYREISNVVQEIKSLPVRKMLFVDDNLTANKRYAHDLMKELI
                     PLHISWGCMSSIEIAKEEELLTEMNNAGCFNILIGFESLNAGSLFETKKKQNRDALIY
                     EDAIKKIHSHGIHITASFVIGFDNDTPEEFDRLYEFTQRTGLSYVNFNILGAPYGSEL
                     QKRLTAEGRMYDIDTDMMGGLFPCIHYYKMSQIELFDKYLETLIKIYSYESVYEKAKV
                     LFGDGYFTKTYNDGSPGTGFIIKLLLKLLKEYIFTSEPYKRRFYHYMISLIRKKKVAI
                     DMGFSHILSMVSYHKHIRKLNEDADQYRQMIKKYDKGAWEKIEEKKEKV"
     gene            complement(1532..>7473)
                     /locus_tag="PKH58_10310"
     CDS             complement(1532..>7473)
                     /locus_tag="PKH58_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNX89467.1"
                     /translation="YYFRSYNSCGWGTQGSATVTINSTPSSVSVSGGGTYCNSATLTA
                     SGGSGGTIYWQGATSGGTSTTNSTNPQTVTTSGTYYFRAYNSCGWGTQGSATVTINTT
                     PSEVTVSGGGTFCNSATLSAGGGSGGTIYWQGNTSGGTSTTNSTNPQTVTSSGTYYFR
                     SYNSCGWGTQGSATVIINPVPTSPILGTITQPTCTVTTGGVVLNGLPATGTWTLTRTP
                     GGTITSGTGTSSTITGLTAGTYTYTVTNSSGCTSLASANIVINAQPTTPTAPTIGTIT
                     QPSCTTATGNVVLNGLPSSGTWTLTRTPGGTITTGTGTSTTITSLVGGTYTFTVTNAS
                     GCTSVSSANVVINAQPTIPSAPTVGAITQPTCAIATGSVILSGLPSTGTWTLTLTPGG
                     TATTGTGTSTTISGLAAGTYTYTVTNSSGCTSIASSNVVINTFSGTPNAPILGTITQP
                     TCTESNGSVVLNGLPTTGTWTLTVTPGGTTTTGIGTSTTITGLAAGIYSYTVTNSSGC
                     TSVASANVIINVQPIIPTAPTIGTITQPTCTVATGGVVLNGLPATGTWTLTRSPGGTI
                     TNGTGASSTITGLTAGTYTYTVTNSSGCTSLASANIIINSQPTTPIAPTIGLITQPSC
                     TTATGNVVLNGLPSSGTWTLTKTPGGTITTGTGTNTTITGLASGTYTFTVTNSYGCTS
                     VASANVVINAQPAPTVSVITTNTSCSGNTGTATANVSNGTPLYTYLWSDGQTTQTATG
                     LGAGSYSVTVTDGNGCIVSSSVNINFPSTYISQTTDTVIIDLFFEQKSINGNNFVFFI
                     DYNNDGKTDMLTRENSSGKIFSYRNTGTTYIIEDTLNANAYSYSANPSSKFYTNLCQF
                     YTDMDNDGNNDLVIYSANHSNCSSNSVRVYWGNASFPYFSDAIYTNITLNYAFCVGAY
                     AVDFDNDGLKDIFVRTAGTGAETMSKNNGNRSFSTVTTINTGRDIGVNFDDYDGDGFA
                     DLCYTKNGWADSQWGIRFNKGLGTGDFNASTIINYSAQRPVDDFVRFAADPLSDTVPD
                     ILFNCSNDGTNGGNFYWGEWSQTLNNFTFDTIITGNNTESTLIQAIDYNSDGYEDVIM
                     RYKTGTNPYYYTTIAYLNDGFGNFNTNDTIIKNAPYSTFQLFKDGNGLKLAAYGNPDT
                     LYVFSVTQIHGGIPTDGLVAHYPFNGNANDESGNALHGIVHNNVVLTTDRNGNPNSAY
                     YFTGGTYDWIEVANNPLLNLGPDFTVSAWVVKTGTNGIIINKGRDISCGTWGLSTNTI
                     GINGTCGTNTGTSVDVTLNEWHMVTGVLDGNNGKLRFYQDAQLIAEVVTNSFTANNNY
                     PIAIGRHCTDQACGSAWPYPFMGKIDDIIIYNRTLNQSEINQLYQSTSAPSVPIGISL
                     GNDTTICSNQTIALDAGAGFNNYHWSTNENTQSISVSNLNAGIHQYTVTVTACGSTAA
                     DTINVNISPTPDSAGIITGITTVCSGQNAVTYTVPSIVNATSYVWTLPGGATGTSNTN
                     SITVDYSDTAVSGNITVKGNNSCGDGAISALTIAVNGYIISGKTLYMGKANAGNPIPN
                     QPTYNSAIYNLDKVIVILKNQYLGTEIARDTSDASGAYEFRCITDGNYTLSYDKYTSD
                     TMQWGNNVNAVDLALLKYLIGHDTLTDPSRSFAAKHKRAANIDNNTTINTIDIARIAS
                     KVGLPYTPARNFPRGNWVALDTSVTVAGSNLNITLKTICYGDYDASSTKYKDSTLTWS
                     MAKSLPDENIILRSDESVTTNNNGYFEVPLRINAKMNEFSAMGLELSYPSEQYKLVSA
                     SMPNTGKKSDAIKINPTLEEIIAANNDLLVTDIDGVIRVVFATTDHFDIAANVELVRL
                     GFRSLNDPGRGELDFNLSGTGLIANQYGEINEDAYLTMPKIFVQGDELEAGFEFAGYP
                     NPFSGDATLTYSIPENGTVKLSVYNAIGELVTLLVNETQIIGTHSAVFSPLNLPAGMY
                     TFKLEFTGSDKSKCIVLKMIH"
BASE COUNT         2102 a         1502 c         1474 g         2395 t
ORIGIN      
        1 acaaaatgcg ttaccgcgaa atttcaaatg tggtgcagga aattaaatca ctcccggtca
       61 ggaaaatgtt gttcgtggat gataacctga cagccaacaa acgttacgct catgatctga
      121 tgaaagagtt gataccgctt catatttcat ggggctgcat gtcgagcatc gaaattgcca
      181 aagaagagga gttgctcacg gaaatgaaca atgccgggtg ttttaatatt cttataggtt
      241 ttgaatcatt aaatgcgggc agcctgtttg aaacaaagaa aaagcaaaac cgcgatgcgc
      301 tgatctatga agatgctatc aaaaagatcc attctcatgg gatacatatc accgcatcat
      361 ttgtcatcgg ttttgataat gataccccgg aagaattcga ccgcttatat gaatttacac
      421 agcgcacagg cttatcttac gtaaacttta atattctcgg agctccttat ggctctgaat
      481 tgcaaaaacg cctgaccgcc gaaggccgca tgtacgatat cgatacggat atgatgggcg
      541 gcttgttccc ttgcatccat tattacaaga tgtcgcaaat tgaattgttt gacaaatatc
      601 ttgagacact tataaaaata tattcctacg aatcagtata cgaaaaagct aaagttcttt
      661 ttggcgatgg atattttaca aaaacatata atgatggcag tccgggtacc ggttttatca
      721 ttaagttact tctaaaactt ttaaaggaat atatttttac ttctgaacca tataagcgca
      781 ggttttatca ttatatgatc tcccttatca ggaagaaaaa agttgctata gacatgggat
      841 tttcccatat tctttcgatg gtcagttatc ataagcatat cagaaaattg aacgaagatg
      901 ccgatcagta caggcagatg atcaaaaaat acgataaagg agcctgggaa aaaatcgaag
      961 aaaaaaaaga aaaagtgtga tcttttttta aaaaaaatac tgcttatctc ctttaaacaa
     1021 gttggcttta atcgcacgaa tataagtctg aatctaatga cagttcttgt tttaaatttg
     1081 agattgccca tccaacggtc acacagagca gcaattcttg taattttata attttaaatt
     1141 ttttctttga atcgcttgtc cctgcctgac tgcctcacgg cagtgaaggc aggcagggaa
     1201 gtgtgacagg cttggccagt cttccctgcc tgcgtgccgg acactccggc aggcagggac
     1261 acgttgttca ttgattaaat ctatcattaa attcttttgg aacaacggct ctgactgacc
     1321 gatacaatta gcccttcatt ccgaaccctg cttaccgtaa cggcagtaca ggcaaggatg
     1381 tagggaagtt cagtcatgta tatttgaata aaccaattaa cttgtcttaa gtggataagc
     1441 aataaaaaat aatcgaatcc accaaaaaaa agagaccgtc ccgatgatga gacagcctct
     1501 tttttaaaag aaaatcagat ttattacagg atcaatgaat catcttcaat acgatgcatt
     1561 ttgatttatc tgaacctgta aattccaatt taaaagtgta catacccgcc ggcagattca
     1621 aaggtgaaaa tactgccgaa tgtgttccaa tgatctgggt ttcatttaca agtagagtca
     1681 ccagttcacc gatagcatta tatacgctca gttttaccgt accgttttca ggaatgctat
     1741 atgtcaaagt agcatcaccg ctgaatggat tgggatagcc cgcaaactca aatccggctt
     1801 ccaactcatc accctggaca aagatctttg gcatcgtaag gtaggcgtct tcatttattt
     1861 cgccatattg attagctatc aaccctgtac cgctcaggtt aaaatccagc tctccgcgac
     1921 ccggatcatt gagcgaacgg aagcctaacc tgaccagttc aacatttgca gcaatatcaa
     1981 aatgatcggt ggtggcaaat actactctga tcactccatc tatatctgtc acgagcaagt
     2041 cattattagc ggcaatgatc tcttccaaag taggattgat ctttattgca tcgctttttt
     2101 tgcctgtgtt tggcatggag gcgcttacca atttgtattg ttcacttggg taactcaact
     2161 ccaggcccat tgctgaaaac tcattcattt ttgcatttat ccgtaatggg acttcaaaat
     2221 atccattatt atttgtagtt accgactcat ctgaccggag aataatattt tcatcaggta
     2281 gtgattttgc catactccag gttagtgttg aatctttgta cttggtgctg gaagcatcat
     2341 aatcgccata acagattgtt ttcagggtga tgttcagatt ggacccggca accgtcacgg
     2401 atgtgtcaag ggctacccag tttcctcttg gaaagttcct ggcgggagta tatggcaacc
     2461 caacctttga agcaattctt gcgatgtcaa tagtattaat agttgtatta ttatcaatgt
     2521 ttgcagctct tttatgtttt gccgcaaaac tcctggacgg atcagtcaat gtatcgtgcc
     2581 ctataagata tttaagtaaa gctaagtcaa cagcattcac attatttccc cactgcatgg
     2641 tatcggaagt gtatttatca taggaaaggg tgtaattacc atctgttata catctaaact
     2701 cataagcgcc cgaggcatca cttgtatctc gggctatttc ggtgcccagg tattgattct
     2761 taagaattac tattactttg tctaaattgt aaatggctga attataagtt ggctggtttg
     2821 gaataggatt gcctgcattg gcttttccca tatataatgt ttttccgctg atgatatacc
     2881 cattaacagc aattgttaat gctgaaatcg cgccatcccc gcatgaatta tttcctttta
     2941 cggtaatgtt gccagaaaca gcggtatcac tataatcaac agtaatacta ttggtattgc
     3001 ttgttccggt agctccaccc ggcagcgtcc agacataaga ggttgcattg actattgatg
     3061 gcaccgtata ggttacagca ttttgcccac tgcatacagt agttattcct gtaataattc
     3121 cggcactatc aggtgtcggg ctaatattta cattaatagt atctgctgct gttgaaccac
     3181 atgccgtaac agtaacagta tattgatgaa taccggcatt taaatttgaa acagatatag
     3241 attgtgtatt ctcgtttgtc gaccaatgat aattattaaa accggctccg gcatctaaag
     3301 ctatagtttg attggaacat attgtggtgt catttccgag cgaaattcca attggcactg
     3361 atggcgctga agtactttga tataattgat ttatttcgga ttggtttaaa gtcctgttat
     3421 atatgataat atcatcaatt ttacccatga aaggataagg ccatgctgag ccacaggcct
     3481 gatcagtaca atgccttcct attgcaatag gataattatt atttgcagta aaagaatttg
     3541 ttacaacttc tgctattaac tgagcgtcct gataaaaacg aagttttccg ttattgccat
     3601 caagcactcc ggtaaccata tgccattcgt ttaatgtaac atctactgat gtcccagtat
     3661 ttgtgccaca agtaccatta ataccaattg tatttgtaga taatccccag gttccgcatg
     3721 aaatatccct gcccttatta ataataatac cattggtgcc agttttaaca acccatgctg
     3781 aaactgtaaa atcaggaccc aagttaagca gtgggttatt ggcaacttct atccaatcat
     3841 aagtacctcc ggtaaaataa tatgctgaat tcggattacc atttctgtca gttgtcaaaa
     3901 ccacattatt atgaacaatg ccatgcaatg catttccgct ctcatcattg gcattgccat
     3961 taaaaggata atgcgctacc aatccatccg taggtattcc tccatgtatt tgagtgactg
     4021 aaaaaacata caaagtatca ggattgccgt atgctgcgag tttcaatcca ttaccatctt
     4081 taaataattg aaatgtagaa tatggtgcat ttttaataat cgtatcattt gtattgaagt
     4141 taccaaaccc atcatttaga taagcaattg ttgtatagta ataaggattt gttccggttt
     4201 tataacgcat aataacatct tcatagccat cgctattgta atcaattgcc tggattaatg
     4261 tagattcagt gttatttcca gttattattg tatcaaatgt gaagttattc agggtttggc
     4321 tccattcgcc ccaatagaaa tttcctccat tagtgccatc attagaacaa ttaaataaaa
     4381 tatctggtac agtatcgctt aatgggtctg cggcaaacct gacgaaatca tcaacaggcc
     4441 tttgtgctga ataatttata attgtagacg cattgaagtc accggttcca agccctttat
     4501 taaaccttat accccattgg ctatcagccc agccattttt cgtataacac aaatcagcaa
     4561 aaccatcacc atcatagtca tcgaaattga ctcctatatc cctgccagta tttattgttg
     4621 ttaccgtaga gaaagatctg tttccattgt ttttactcat tgtttctgca ccggttccgg
     4681 cagttctgac aaatatatcc ttcaaaccat cattatcaaa atcaacagca taagcaccaa
     4741 cacagaatgc ataattcaat gtgatatttg tgtagattgc atcagaaaaa tacggaaaac
     4801 tggcatttcc ccaatatact cttacactat ttgatgaaca gtttgaatgg ttggcagaat
     4861 aaattaccaa atcgttattt ccatcattat ccatatctgt ataaaactga catagattgg
     4921 tataaaattt actgcttgga tttgccgaat atgaataagc attcgcattt aaagtatctt
     4981 caataatata tgttgttcca gtgttacgat aggaaaatat tttaccgctt gaattttcac
     5041 gtgtaagcat atcagtctta ccatcattat tgtaatcaat aaaaaacacg aaattattac
     5101 cgtttatact tttttgttcg aaaaataaat caataatcac agtgtcggta gtctgagata
     5161 tatatgtact tggaaaatta atatttacgg atgaactcac aatgcatcca tttccgtcag
     5221 taacagtaac cgaatatgat ccggctccca atcctgttgc ggtttgtgta gtttgcccat
     5281 cactccataa gtatgtatat aatggtgtcc cattggaaac attagctgtt gcagtgcctg
     5341 tatttccgga acaagaagta tttgttgtaa ttactgagac agtaggtgcc ggttgagcat
     5401 taataaccac attagccgat gcaacagaag tacatccata tgaatttgtc actgtaaagg
     5461 tatatgttcc ggatgcaagc cctgtaatag ttgtgtttgt tccagttcct gtcgtaattg
     5521 tgcctccggg tgttttggtt aatgtccatg ttcctgaaga tggcaaacca tttaaaacca
     5581 catttccggt agccgttgtg catgatggtt gtgtaattaa tccaattgtt ggcgcaatcg
     5641 gtgttgttgg ttgggaattg ataataatat ttgcagatgc taatgaagta caccctgatg
     5701 aatttgttac tgtataagta tatgttcccg cagtgagtcc cgttattgtc gaacttgcac
     5761 cagtaccgtt ggtaattgta ccgcctggag atcttgtcaa tgtccatgtt cctgttgcag
     5821 gtaatccgtt taaaacaaca cctcctgttg caactgtaca tgttggttgt gtaattgttc
     5881 ctattgttgg agctgtgggt atgatcggtt gaacattgat gatcacatta gctgatgcaa
     5941 cagaagtaca ccctgacgaa ttcgtcacag tataggaata tattccggcg gcaagccctg
     6001 ttatagttgt gcttgttcct attcccgtcg tagtagttcc tcctggggtc acggttaatg
     6061 tccaggtacc tgttgttggt aaaccattca aaaccacact tccgtttgat tcggtgcatg
     6121 tgggctgcgt aattgttccc aatattggtg catttggtgt tcctgaaaat gtgttgataa
     6181 caacatttga cgatgctatt gaagtacacc ctgatgaatt cgttacagta taggtgtagg
     6241 ttccggcagc cagtcctgaa attgttgtgc ttgttcctgt tcctgttgta gctgttcctc
     6301 caggtgtcaa agttaatgtc caggttcctg ttgatggcag cccgcttaaa attacacttc
     6361 ctgttgctat tgcgcatgtg ggttgtgtaa tagctcctac tgtgggcgct gatggtattg
     6421 ttggttgggc attgattact acattagctg aacttacaga agtacaccct gatgcattgg
     6481 taactgtaaa ggtatatgta ccgccaacta agcttgtaat tgttgtgctt gttcctgttc
     6541 cggtggtaat tgtgcctccg ggtgtcctcg ttaatgtcca ggttcctgaa gatggcaaac
     6601 cattcaaaac tacatttccg gttgccgttg tgcatgatgg ctgtgtaatt gttccaattg
     6661 taggagctgt aggtgttgtt ggttgggcat tgataacaat atttgcagat gccaatgaag
     6721 tacatcctga tgaatttgtt accgtataag tatatgtccc agcagtaagc cccgttattg
     6781 ttgaactcgt accagtgccg gaagtaattg tgccgcctgg agttcttgtc aatgtccatg
     6841 tccctgttgc aggtaatccg tttaatacga cacctcctgt tgtaaccgtg catgttggtt
     6901 gcgtaattgt acctaaaatt ggagatgttg gtacaggatt aattattact gttgcgctgc
     6961 cttgagttcc ccatccgcaa ctgttatatg acctgaaata ataagttcct gaagatgtta
     7021 cggtttgtgg atttgttgaa ttagttgttg atgttcctcc gcttgtattg ccttgccaat
     7081 aaatcgtgcc tccgcttcca ccgcctgccg atagtgttgc gctgttgcag aatgttccac
     7141 caccgctaac tgttacctct gatggcgtgg tatttattgt taccgttgcg ctgccctgtg
     7201 ttccccatcc gcaactgttg tatgctctga aataatatgt tcctgatgtt gttacagttt
     7261 gtggatttgt tgaattagtt gttgatgtcc ctccgcttgt agcgccttgc cagtatatcg
     7321 tgccaccact tcctccactt gcagttaatg ttgcactatt acagtatgtt ccgccaccac
     7381 ttaccgatac tgatgaaggt gtcgaattta ttgttaccgt tgcgctgcct tgagttcccc
     7441 atccgcaact gttatatgac ctgaaataat aag
//
LOCUS       DAOPYB010000150         7403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12120_length_7403_cov_7.399156, whole genome shotgun sequence.
ACCESSION   DAOPYB010000150 DAOPYB010000000
VERSION     DAOPYB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12120_length_7403_cov_7.399156"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            706..1029
                     /locus_tag="PKH58_10315"
     CDS             706..1029
                     /locus_tag="PKH58_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX89468.1"
                     /translation="MLFANKIRQLREERKLLQRHLAAVLDMDTPMFSKIERGERPAKR
                     EQVIAIAKILTTDENELLTLWLADKILDVIENETELADQALNVAIENKNHLTGSPKLP
                     TPNRF"
     gene            1081..>1694
                     /locus_tag="PKH58_10320"
     CDS             1081..>1694
                     /locus_tag="PKH58_10320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNX89469.1"
                     /translation="MIKDIIQNNEALTPNSQTLEVLQAHFPQCFNKEGFFDVDKFKDL
                     IKADVDITHEGYDLNFLGKNYAKLLASIDTTTVIQPDVEHNAKPENANSQNLYISGDN
                     LDGLKHLLKSYTGAVKCIYIDPPYNTGSDGFVYNDNFNFTPQELIDKLSISEEQAQKI
                     FDLTTKGRASHSAWLTFMYPRLLLARDLLTPDGVIFISIDDNEQA"
     assembly_gap    1695..1794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1795..3110
                     /locus_tag="PKH58_10325"
     CDS             <1795..3110
                     /locus_tag="PKH58_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNX89470.1"
                     /translation="LLTPDGVIFISIDDNEQANLKLICDTVFGEENFLANVVWQRTYA
                     PISMKKYFSESHEYLYVYTKEKDKFNLNLLPRTEKQNNDYKNPDNDPRGVWKGGNLTV
                     GPAVEKQIYEIIGPTGKSFYPPKGYCWRFTKERFEELRKDDRIWFGADGNNSPLPKLF
                     LTEVQDGVTPMTLWTFQEVGHNQESTRELRDLFDNAVFTNPKPTRYIERILGLGSNPN
                     DIIVDFFSGSGTTAHSILNLNSKDGGNRKFIMVQISEPSKIDSEAEKAGFKTLDQIGI
                     ERIKRAAAKIKSETNADIDYGFKHYTLVEPSEDTIDKLEEFKDTELFANNDTLSLFGK
                     ETVLETWLVKDGYGFGAKVEEVKLSGYTAYLCGKHLYFIEAGINENDMVALLDRYQQE
                     PSFSPENIVVFGYSFNFSQTEMLRKNLFVLRDTHKNLKANFDIRY"
     gene            3276..3851
                     /locus_tag="PKH58_10330"
     CDS             3276..3851
                     /locus_tag="PKH58_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX89471.1"
                     /translation="MKQDFFEPGVVYHVFNRGNNKEDIFKEEKNYAYFLSLVKKYLLP
                     VAEIYSYCLLKNHFHLALRIKDEHSLEDKYKQKPYIAFSNLFNAYTKSINKLYNRTGS
                     LFQEHLHRKRVEDEEYLTQLVAYIHLNPVKHEFSDDFKNYRYSSYQAYKTGKTTNIST
                     QYVMSLFGDITNFEYWHDLNKLYLEDKMEDI"
     gene            4197..7151
                     /locus_tag="PKH58_10335"
     CDS             4197..7151
                     /locus_tag="PKH58_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HNX89472.1"
                     /translation="MELILQTGLSHQQKAVDSIADVFRNTGISQPNQYYSNPGISPDS
                     ILLGNIQEVQKANELHNSLRVLSKEVTPLHLDIKMETGTGKTYVYTHSIYELHHRYGI
                     NKFIVVVPSLPIKAGAEQFLGDGYVQKHFENTCGYGAKIELYTLKAQAKKKGKTFFPS
                     AVRAFVEGSNQTSNRIYVLLVNMQLLTNGNMLSRSDYDFGVHGYYRPFDALAATKPFV
                     IMDEPHRFQRDQKAYERITEELKPQCIVRFGATFPDITVGKGKNKTTKKDYLNLLYDL
                     NACDSFNQNLIKGVAKEHFEPLSSRDEKIKITTIESKTSVTFNHISANGRRSYTLQTG
                     DSLSVISEELKGLSITGIGKNFVEFSNGQEKFTGEEFTVDIFSDSYQEQMIRLAIQRH
                     FETERANFDRPCKIKTLALFFIDNIDSYRGDSNGDKWLHTIFEQLLEERIEYELTQNC
                     SPEYRAYLEASKADISACHAGYFAKDNTDTDDNIANEVDEILHNKKKLLSIVTEDGSL
                     NTRRFLFSKWTLKEGWDNPNVFTIVKLRSSGSDNSKIQEVGRGLRLPVDEFGNRISNE
                     EFMLNYIVDFTEADFANRLVAEINAELPAEQATHISTEELERVAKLRNVDTNQFMIEL
                     MTKGYIDFSRKINIDKINDFYQDYPEFNMPRLAGTKVIDKNKQKKNTVKVREARFNEL
                     KELWAAINKKYVLFFNNDVEQSIDKDIQTLLKDGVFGLQEITTDRQTIQAGKDNVEVV
                     GEAGVTYLISGRKLPYNAFLKRINKATSIPINTLHNAISIYAKSENGFNQAYINESSA
                     ARFISKFNDWKYENLKGRFNYKQTEYTPTSTKLTNADGSVRDEVVQGEIGTNLLKGEP
                     SAKYLYDVIAYDSPLECKNIMEDIQEVVVYGKIPRLSISIPTIGNDSYSPDFMYVVKK
                     ANGEKELNIVVETKQVEGKSSLRGEEAMKISCAEEFFNQLTLDGYKVSFKTQLNNNGV
                     KSIIEEIVRGNN"
     gene            complement(7210..>7403)
                     /locus_tag="PKH58_10340"
     CDS             complement(7210..>7403)
                     /locus_tag="PKH58_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89473.1"
                     /translation="KKQTSFRIIGTGRGFQFVSAVRPPSGSLQGGRELSIVRKFQVYI
                     LRTMRMEPQYARCGVDSGL"
BASE COUNT         2468 a         1360 c         1443 g         2032 t
ORIGIN      
        1 attgttcttt gattaaaacc aatcacatga atcgcgctcc atgatttttg acaaaggtcg
       61 cgaatcacgt gcttacggtt ccatgttttt tgacaacggt cgtgaatcat gtgattctcg
      121 ttccatgttt tttgacaacg gtcgtgaatc acgtgattcc tgttcaatgt tttttgacaa
      181 gggttgcgaa tcacgtgatt tcggttcaat gttttttgac aacagttgtt atttccgcta
      241 attagggttc tttttttctc agacctaaca ggttttcaaa acctgtcagg tctgagaaaa
      301 acctgcaata tcctcattgt gtaataatta ctcataaatg ttgagatttt atttaacaaa
      361 ttaaaaagat aatgtctata tttgtcagag caagacagaa agccttgttc cttattttat
      421 gtcagttatt ggtcggaggt attttgtctg aaaagaaact acttaaagtc tggagacttt
      481 cttttagcat gatatggtct ggaggttacg ccaaacaaag tttataagtt gaaaactttt
      541 ctttttatgc ccgttttggt tttaaataat tgtctatctt tgttcaaatt cagacaatcc
      601 aaaaagcaaa aagtcttgca atcaaaagca aatgttgagc ttgtttcaat ctgcgctgtg
      661 tgcaatcggc tttatctgaa acaaaaagtc taaaacaaaa cgattatgtt atttgcgaat
      721 aaaattcgac aactaagaga agagcggaag ttgctgcaac gccatttggc tgcggtactg
      781 gatatggata caccgatgtt tagcaagatt gaacgtggcg aacgtccggc taaacgtgaa
      841 caagtgattg ccattgctaa aattcttact accgatgaaa acgaattgct aaccctttgg
      901 ttggcggata aaattttaga tgtaatagaa aacgaaacag aattagcaga ccaggcattg
      961 aacgtagcta ttgaaaataa aaaccaccta acaggttcac caaagcttcc cacacctaac
     1021 aggttttaga aacctgttag gtgtgggaga gaaaaacaaa acacagaaca atccaacaat
     1081 atgataaaag atattatcca aaacaacgag gctttaactc ccaacagcca aacactggaa
     1141 gtgttacaag ctcatttccc ccaatgtttc aacaaagaag gttttttcga tgtagataaa
     1201 ttcaaagacc tgattaaagc cgatgtggac ataacccacg aaggctacga cctcaacttt
     1261 ctggggaaaa actatgcgaa attattggct tcgatagata ctaccaccgt tattcaaccc
     1321 gacgtggagc acaatgcgaa acccgaaaac gccaacagcc aaaatctcta tatcagtggc
     1381 gataacttag acggtctgaa acatttgcta aaatcataca ccggcgcagt gaaatgtatc
     1441 tatatcgacc cgccttataa tacaggttcc gacggttttg tgtataacga taacttcaat
     1501 tttacgcccc aagagttaat cgacaaacta agtattagcg aagaacaggc acagaagatt
     1561 ttcgacctca ctaccaaagg acgtgcttcc cattcggcat ggcttacgtt catgtacccc
     1621 cggttgttgc ttgcacgtga tttgcttacg cccgatggag ttatctttat ttctattgat
     1681 gacaatgaac aagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatttgc
     1801 ttacgcccga tggagttatc tttatttcta ttgatgacaa tgaacaagct aacctgaaat
     1861 tgatttgtga tactgtgttt ggggaggaga attttttagc taatgttgtg tggcaaagga
     1921 catatgctcc tattagtatg aaaaaatatt tttctgaaag tcacgaatat ttgtatgttt
     1981 atacaaagga aaaggacaaa tttaatttga accttttgcc tcgcacagag aaacaaaata
     2041 acgattataa aaatccagat aatgatccac gaggtgtttg gaaaggcggt aatttaacag
     2101 ttggaccagc tgttgaaaag caaatatatg aaatcattgg tcccacaggt aaatcattct
     2161 atccgccgaa aggatactgt tggcgtttta cgaaagaaag gtttgaagag cttagaaaag
     2221 atgatagaat atggtttggt gcagatggga acaattcacc attaccaaag ttatttctta
     2281 cagaggtaca agatggtgta actccgatga ctttatggac ttttcaggaa gtaggacata
     2341 atcaggaatc tacaagagaa ttaagagatt tgtttgacaa tgccgttttt acaaatccta
     2401 aaccaacgag atatattgaa agaattttag gacttgggtc aaatccaaat gatattattg
     2461 tcgatttttt ctctggttca ggcactaccg cacattccat actaaatctt aattcaaaag
     2521 atggtggaaa cagaaaattc attatggtac aaatatcaga accttccaaa attgatagtg
     2581 aagcagaaaa ggctggattt aaaacacttg atcaaatcgg tatagagcgt ataaaacgtg
     2641 ccgccgcaaa aatcaaatct gaaacaaacg ccgatattga ctacggtttt aagcactaca
     2701 ccttggtaga gccgtcggaa gatacgattg ataaattgga agaatttaag gatacggaac
     2761 tatttgccaa taacgatacc ttatcgctgt ttggcaaaga aactgtgctc gaaacatggt
     2821 tggtaaaaga cgggtatggc tttggagcaa aagtggagga ggtaaaatta tcgggttaca
     2881 cagcttactt gtgcggtaaa cacctctatt ttattgaagc gggaatcaac gaaaacgaca
     2941 tggtggcatt gctcgaccga taccagcaag agccgtcgtt cagtcccgaa aacattgtgg
     3001 tttttggtta cagtttcaat ttctcacaaa ctgaaatgct tcgcaaaaac ctgtttgtgc
     3061 tgcgcgatac gcataaaaac ctgaaagcta acttcgatat cagatattaa gttttcacaa
     3121 cacctaacag gtttcaaaaa cctgttaggt gtaacgaagg taacaaacag cctaacctaa
     3181 caggttttca aaaacctgtt agttgtaacg aaggtaacaa acagcctaac ctaacaggtt
     3241 tcaaaaacct gttaggtttc accaatacaa ataagatgaa acaagatttt tttgaacctg
     3301 gcgttgtcta tcacgtattc aaccggggca acaataaaga agatattttc aaggaagaaa
     3361 aaaactatgc atattttctt tctttggtca aaaagtacct gttgcctgtt gccgagatat
     3421 attcttattg cctgctaaaa aatcattttc atttggcatt aagaatcaaa gatgagcatt
     3481 cattagaaga taaatataaa caaaaaccgt atattgcgtt ttccaatctg tttaatgctt
     3541 acacaaagtc gataaataaa ttgtacaacc gtacaggaag cctttttcag gaacatttac
     3601 accgcaaacg tgttgaagat gaggagtacc tgacacaact cgtggcttat attcatttga
     3661 atccggtaaa acatgagttt tcggacgact ttaaaaacta tcgttattcg tcttatcaag
     3721 cttacaagac cggaaagaca accaatataa gtacgcagta tgttatgagt ttgtttggag
     3781 atattactaa ttttgaatat tggcatgatt taaacaaatt gtatcttgaa gataaaatgg
     3841 aagatattta ataacaccta acaggttttc aaaaacctgt taggtgtaag aatgaagata
     3901 aaatggaaga tatttaataa cacctaacag gttttcaaaa acctgttagg tgtagatgga
     3961 ataagcagca gttagccgaa ctttttgtca ccactaagta aaacataggt caacatatat
     4021 cttctttact gaaagagaat gtgttgagta aaaaatcagt tgtaaagaat tactttataa
     4081 ctgccgccga cggcaaacaa ggaacgaaag gtcaaacgaa gaaaataaac ttacacctaa
     4141 caggtttcaa aaacctgtta ggtgtaagaa aaatgttagg tataagaaaa aaatacatgg
     4201 aactaatttt acaaacgggg ttgtcgcacc agcaaaaagc agttgactcc atagctgatg
     4261 ttttccggaa tacgggtatc agccagccga atcaatatta tagcaatcca ggcatttctc
     4321 ccgatagtat ccttttgggg aatatacagg aagttcagaa agccaatgag cttcacaact
     4381 cattgcgtgt tctttctaaa gaagtgacac cgctgcattt ggatattaaa atggaaaccg
     4441 gcacggggaa aacatacgtt tacacccatt ccatttatga gctacaccac cgttatggta
     4501 ttaataagtt tattgtggta gttccttcgc tcccaatcaa ggcaggtgcc gaacaattcc
     4561 ttggcgatgg atatgttcaa aaacactttg aaaacacctg tggttatgga gctaaaatcg
     4621 aactatacac actaaaagca caagcgaaga aaaaaggtaa aacctttttc ccgagtgctg
     4681 tccgtgcttt tgtggaagga tcaaatcaaa caagcaaccg gatttatgta ttgcttgtca
     4741 atatgcagct attgacaaac ggaaatatgc tttctcgttc cgattacgat tttggcgttc
     4801 atggttatta ccgtcccttt gatgcgcttg cagcaacaaa gccgttcgtg attatggatg
     4861 aaccacaccg ctttcagcgc gaccaaaaag cgtatgaacg gataactgaa gaattaaagc
     4921 cacaatgtat tgtccgcttt ggagctacgt tccccgatat tacagtaggt aaaggaaaaa
     4981 acaaaacaac gaagaaagat tatctcaatc ttttgtacga cctcaacgct tgcgattcgt
     5041 tcaatcaaaa tcttatcaaa ggcgtagcaa aagaacattt tgaaccgcta tcgtccagag
     5101 atgagaaaat taaaataact accattgaaa gtaaaacttc agtgacattt aatcatattt
     5161 cggctaatgg tcgcaggtct tacacattgc aaacaggcga ctcactgtct gtgatcagcg
     5221 aagaactcaa agggctctct attaccggaa taggtaagaa ttttgtggaa ttttccaatg
     5281 gtcaggaaaa gttcacgggt gaagaattta ctgtcgatat tttttccgat tcgtaccagg
     5341 aacaaatgat tcgactggct atccaacgcc atttcgaaac ggaacgagcc aacttcgacc
     5401 gcccttgtaa gataaaaaca ctcgcacttt tctttatcga caatattgat tcatatcgtg
     5461 gtgatagcaa cggcgataaa tggctgcaca caatctttga gcaactttta gaagaacgga
     5521 ttgaatacga gctaacgcaa aattgctcgc ccgaatacag ggcatatctt gaagcaagca
     5581 aagccgacat ttcggcttgt catgccggat attttgccaa agacaatacc gataccgatg
     5641 ataatatagc aaatgaggtg gatgaaatac ttcacaacaa aaagaagcta ttgtctatcg
     5701 ttacggagga tggttcgttg aatacccgcc gtttcttgtt ctccaaatgg acgctcaaag
     5761 aaggatggga caacccgaat gtgtttacca ttgtcaagct ccgttcgagt ggtagcgata
     5821 acagcaaaat acaggaagtg ggacgaggct tgcgtttgcc cgtagatgag tttggtaatc
     5881 gtatatcaaa tgaggaattc atgctgaact acattgttga ttttaccgaa gccgattttg
     5941 ccaatcggtt ggtggcggaa attaatgcag aattacctgc cgaacaagct actcatattt
     6001 caacggaaga gttggaacgg gttgccaaac ttcggaacgt tgataccaac caatttatga
     6061 ttgagttgat gacaaaaggc tatattgact tttcaagaaa aatcaatata gataaaatca
     6121 acgattttta tcaggattat cccgagttca atatgccacg tcttgcaggc actaaagtaa
     6181 ttgacaagaa taaacagaaa aaaaataccg ttaaagtccg ggaagctcgt ttcaatgaac
     6241 tgaaagaact gtgggcagct atcaacaaaa aatatgtgct tttcttcaat aacgatgtag
     6301 aacaatccat agataaagac attcaaaccc tgctgaaaga cggtgtattt gggctgcagg
     6361 aaataaccac cgaccgccaa accattcagg caggaaaaga caatgtggaa gtcgttggcg
     6421 aagcaggcgt aacttatttg ataagtggca gaaaactgcc ttacaatgct tttttgaagc
     6481 gcattaataa agctacttct attccaatta acacgcttca caatgccatt tctatatatg
     6541 ccaaatccga aaacggattt aatcaggcat atatcaacga aagttcagca gcaaggttta
     6601 tatctaaatt caacgattgg aaatacgaaa atttgaaagg tcgtttcaat tacaaacaaa
     6661 ctgaatatac acccacttca actaaactaa caaatgcaga tggctcagtc agagacgaag
     6721 tggttcaggg tgaaataggt acaaatcttt tgaagggcga accgtcagct aaatatcttt
     6781 atgatgtaat tgcttatgac tctccattgg aatgtaaaaa tatcatggag gatattcagg
     6841 aagttgttgt atatgggaaa atcccacgtc tcagcatttc tatccctacc attggtaatg
     6901 attcttatag tcctgacttt atgtatgtcg taaaaaaagc caacggagaa aaagaactga
     6961 atattgttgt tgaaacaaaa caagtcgaag gaaaatcttc tttacgtggc gaagaagcta
     7021 tgaaaataag ctgtgccgaa gaatttttca accaactaac tttagacggc tacaaagtat
     7081 cctttaaaac tcaactgaat aataatggcg tgaagtcaat tattgaggag attgtaaggg
     7141 ggaacaacta aatgctataa aatggctaat ctcggattta aaacccggac tacgtctgga
     7201 aaaaacaact taaagtccgg agtctacacc acaccgggcg tattgcggtt ccatccgcat
     7261 tgtgcgtaaa atatacacct gaaacttacg gacaatgctc agttctctcc ctccctggag
     7321 ggagccggag ggaggtctga ctgctgaaac aaactggaaa cctctacctg tcccgataat
     7381 cctgaatgat gtttgttttt ttt
//
LOCUS       DAOPYB010000151         7399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12131_length_7399_cov_10.009532, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000151 DAOPYB010000000
VERSION     DAOPYB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12131_length_7399_cov_10.009532"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PKH58_10345"
     CDS             <1..1416
                     /locus_tag="PKH58_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89474.1"
                     /translation="FHDNHVAVMEKKVEIMVERFREITLTKIDGKAKAMVVSPSRLHA
                     VKYFMLIKDYCKKQGYGDIKPMVAFSGSISFQNEEYTETKLNSSEDQKITESQLPIYF
                     NSDLFNVLVVAEKYQTGFDEPLLHTMFVDKKLKGVKAVQTLSRLNRSAKGKTDTYVFD
                     FVNTAEDIKKSFSTFYEDTLLDSPVDVNYVYTFKNDLDQFHLWNTEDEEKVYQIYAKK
                     DQEIKDLGKLSSALKPALNAYSILTEENQFKVRFLLKNFNRFYAYMAQIVRTFDKELY
                     KTYVFTEFLYKFLPKNPHEKVNLDDKIALKYNQLKETFSGTIELNPTIKDKTIKGEKP
                     TEGQKPETRKDLLENIIDKINLMFQGQFTEADRVIVETIYDKLKEQGKQLKKQAKNND
                     EQMFENNIFPQIFDKVAQSCYMEQMDAFAKLFENDQFYKHVMNEIGKAMYFNMRNEGK
                     KPNEYQIERKEMLSAAEPEIV"
     gene            1436..1729
                     /locus_tag="PKH58_10350"
     CDS             1436..1729
                     /locus_tag="PKH58_10350"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HNX89475.1"
                     /translation="MEYQIPELPLSIDLETKSVLKQLNAANRKLAELKGVALTIPNET
                     ILINTLALQEAKDSSAVEKISLQHTMTCIVQHQIRKALLFQQRPRKYFLMLKP"
     gene            1648..2559
                     /locus_tag="PKH58_10355"
     CDS             1648..2559
                     /locus_tag="PKH58_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNX89476.1"
                     /translation="MYRAASDTKSLAISAATKEVFSYVEALKHGFFLVRKTKLLTNTS
                     IKDIQQVLEHNNAGFRRVPGTTLKNQLGEIVYTPPQNADEIEFYMANLEKFINDDTIS
                     ELDPLIKMAIIHHQFESIHPFFDGNGRTGRIVNILYLVIKELLDIPILYLSRYVIRNK
                     GEYYRLLQDVRVNNSWEEWIIFMLKGVEETAEQTIYLVKQVSIMMAEYKFKMRPVFGK
                     VYRHELLNNLFNHPYTKIEFIMNDLGIKRLTAAKYLDMLVDLELLKKIKVGRENYYLN
                     TKLIDLFMNFSVEVQDNELNRIITTHE"
     gene            2738..2914
                     /locus_tag="PKH58_10360"
     CDS             2738..2914
                     /locus_tag="PKH58_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89477.1"
                     /translation="MENYFKDNDGQNENYQQQKLTHEEIRQFQQFSEISDKEIEDISD
                     LTYELAIVALKVID"
     gene            complement(3079..3152)
                     /locus_tag="PKH58_10365"
     tRNA            complement(3079..3152)
                     /locus_tag="PKH58_10365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3116..3118),aa:Arg,seq:ccg)
     gene            complement(3261..3941)
                     /locus_tag="PKH58_10370"
     CDS             complement(3261..3941)
                     /locus_tag="PKH58_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNX89478.1"
                     /translation="MIYSYVLFGLIALVSWIVQANFQSKFKKYSRIPLPSGMTGRDVA
                     VKMLRDNGITDVQVISTAGQLTDHYNPANKTVNLSEAVYASDSVAAAAVAAHECGHAV
                     QHATAYAPLTMRSKLVPVVSFASSWVQWILLGGILLLQVFPSLLFFGIILFGLTTLFS
                     IITLPVEINASQRAVAWLSNAGITNYETRDAATGALKSAAYTYVVAALSSMATLFYYI
                     MIFMGRRD"
     gene            complement(4167..5435)
                     /locus_tag="PKH58_10375"
     CDS             complement(4167..5435)
                     /locus_tag="PKH58_10375"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNX89479.1"
                     /translation="MKIDVLLGLQWGDEGKGKVVDVLTPSYDVISRFQGGPNAGHTLE
                     FEGRKFVLRSIPSGIFQGDKINVIGNGVVLDPALFKEEVEALEKSGHALTDRLRISKK
                     AHLILPTHRLLDMAYESAKGSGKIGTTGKGIGPTYTDKVSRNGLRVGDLLHNFDEKYK
                     AAINRHLDILKHYQIEFDLPALEKVWFEGIEKLKEFQFIDSEHFINDTLKSGKKVLAE
                     GAQGTMLDIDFGSYPFVTSSNTICAGACTGLGVAPNKIGDVYGIFKAYCTRVGSGPFP
                     TELFDHDGDEMRRIGFEFGSVTGRPRRCGWIDLVALRYAIMINGVTQLIMMKADVLDS
                     FETIKACVAYKIDGVETEDFPFDLNDGVEPVYAELPGWKTNLTSIKSEDEFPEELNAY
                     LDFLEEELGVPVKIVSVGPDREQTIIRHQD"
     gene            complement(5503..5970)
                     /locus_tag="PKH58_10380"
     CDS             complement(5503..5970)
                     /locus_tag="PKH58_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNX89480.1"
                     /translation="MKEENSHEELMQTFTDYLVKNKYRKTRERYAILDYIYKHQGHFN
                     AELIYNQMPKEYQVSLATVYNTLELLQECNLIVKHQFGEQMAQYEKTFGIIFHHHLVC
                     TSCGKIREVPDVTISKLLQKKKIPRFEPSRYTLYVYGLCSKCSAQQKNGVKKL"
     gene            complement(6027..6710)
                     /locus_tag="PKH58_10385"
     CDS             complement(6027..6710)
                     /locus_tag="PKH58_10385"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNX89481.1"
                     /translation="MKYYIQNEQIDNTIKEIRKKIRLSMNGVVSDKMKESGISYKQNF
                     GVDIPRLKQIASGYDQSKDLAQRLWALQIRETMILATLLHPVDSFTSEMADEWLVSLT
                     HPEIVEQCSMNLFAKLPFAVELSIRWAKSDKLWHQITAFNLGARIWKSFEASQSDELI
                     HRALEISDTAEYHLYKSIALILSRLCRTGKELSDRIYEEISCMKNSHHPSKKYIFSEV
                     SQEISFLNF"
     gene            6795..6870
                     /locus_tag="PKH58_10390"
     tRNA            6795..6870
                     /locus_tag="PKH58_10390"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6830..6832,aa:His,seq:gtg)
     gene            complement(6930..>7399)
                     /locus_tag="PKH58_10395"
     CDS             complement(6930..>7399)
                     /locus_tag="PKH58_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89482.1"
                     /translation="YTTLVNCGSDPLRYIEKNYQDNAAAYIGKTIGFWADQSELQLSG
                     SKYTSIEHSPYVVNKNLLGKIESLCLDVIYKDGVNDYIYYIYIYIDDTTPLSYEEEGK
                     LVGDYDDYWEPKFYNFYKNAKIKKIEVYKEINDKFVNNYRRPSTTTIPIGEPE"
BASE COUNT         2348 a         1425 c         1405 g         2221 t
ORIGIN      
        1 ttccacgaca atcacgttgc cgtgatggaa aagaaggtgg aaattatggt agaacgattt
       61 cgtgaaatta ccctgacaaa gattgacgga aaagccaaag caatggtagt ttcaccttcc
      121 cgacttcatg ccgtgaaata cttcatgctg ataaaggact attgtaagaa acaaggttat
      181 ggcgatatta aaccgatggt ggcattttcg ggcagtatca gttttcaaaa cgaagaatac
      241 accgaaacaa agctgaacag cagcgaagac caaaagataa ctgaaagtca gttgcctatt
      301 tatttcaatt cagacttatt taatgtgtta gtggtagctg aaaagtatca gaccgggttt
      361 gacgaaccat tacttcacac catgtttgtg gataagaaat tgaaaggtgt gaaagctgtt
      421 cagaccttat cccgattgaa ccgaagtgcc aaaggcaaaa cggatactta tgtgtttgat
      481 tttgtcaata ctgccgaaga tataaagaaa tcgttttcaa ctttctatga agataccttg
      541 ttagatagcc ctgtggatgt aaattacgtc tataccttca aaaatgacct tgaccagttc
      601 cacctttgga acaccgagga tgaagaaaaa gtatatcaga tatatgccaa gaaagaccag
      661 gagataaagg acctgggtaa gttatcaagt gccctgaaac cagccctgaa tgcctattca
      721 attttgacgg aggaaaacca attcaaggtt cgttttctgt tgaagaactt caatcggttc
      781 tatgcgtata tggcacaaat tgtcagaacc tttgataagg aattgtataa aacgtatgtc
      841 tttacagaat ttctgtacaa gttcttgcca aagaacccgc acgaaaaagt caatttggat
      901 gataaaatag cgttgaaata caatcaattg aaagaaacgt tcagcggaac aattgaacta
      961 aatccaacaa tcaaggataa aactattaaa ggcgagaagc ccaccgaagg tcagaaaccc
     1021 gaaacacgaa aagacctgct tgaaaacatt attgataaga taaatctgat gttccaaggg
     1081 caattcaccg aagccgacag ggttattgta gaaactatct atgacaaact gaaagaacag
     1141 ggcaagcaat tgaagaagca agccaaaaac aacgatgagc aaatgtttga aaataacatt
     1201 tttccacaaa tctttgacaa agtagcccaa agctgctaca tggaacagat ggatgccttc
     1261 gccaagttgt ttgaaaacga ccaattctac aagcatgtga tgaatgaaat tggcaaagct
     1321 atgtatttca atatgcgaaa tgaagggaaa aaaccgaatg aatatcagat agaaagaaaa
     1381 gaaatgctat cggcagcaga accagaaatt gtataagcaa ctaatatgac ataatatgga
     1441 ataccaaata cctgaattgc ctttgtcaat tgatttagaa accaaaagtg tcttgaagca
     1501 attgaatgct gcaaaccgca agttggcaga attgaaaggg gttgctttga caattccaaa
     1561 tgaaaccatt ttaatcaata cacttgcctt acaagaagct aaagatagtt cggcagtaga
     1621 aaaaatatcg ttacaacaca cgatgacttg tatcgtgcag catcagatac gaaaagcctt
     1681 gctatttcag cagcgaccaa ggaagtattt tcttatgttg aagccttgaa acatggcttc
     1741 ttcttggttc gtaaaactaa attattgact aatacatcaa ttaaagacat tcaacaagtt
     1801 cttgaacata acaatgctgg atttcgtagg gttccaggca caactttgaa aaatcaattg
     1861 ggtgaaattg tatatacacc accacagaat gccgatgaaa ttgaatttta tatggcaaat
     1921 cttgaaaaat ttatcaatga cgatacaatt tccgagcttg acccgttgat taaaatggcg
     1981 ataattcacc atcagtttga aagtattcat cctttctttg acggcaatgg taggacaggt
     2041 cgtattgtca atattcttta tcttgttatt aaagaattac ttgacatccc aattctgtat
     2101 ttaagtaggt atgttatacg aaacaagggt gaatattatc gccttcttca agatgttcgt
     2161 gtaaataatt catgggagga atggattatt tttatgctta aaggggttga agaaacagca
     2221 gaacaaacta tatatctggt taaacaagtt tcaattatga tggcagaata taaattcaag
     2281 atgcgtcctg tatttggaaa agtatatcgt catgaactac ttaacaactt atttaatcat
     2341 ccttatacaa agattgaatt cataatgaac gacctgggca ttaaacgttt aactgctgcc
     2401 aaatatcttg atatgttggt agatttggaa ttgttgaaaa aaattaaagt gggaagagaa
     2461 aactattacc tgaatacaaa actaatagat ttgtttatga acttttctgt tgaagtgcag
     2521 gataatgaat taaacagaat aataacaacg cacgaataat atgtatgcga tttgccgttt
     2581 tcatatacat attatcatga tttgtatata gtttttgaaa actgcataca taaataaaat
     2641 atgtgcatac ataaacaaga ttttttggat atattatttg tggctaatat tatcctgttt
     2701 tttctgaaaa gtccagaaat aatagtcatt tgtaattatg gaaaactact tcaaagataa
     2761 tgatggtcaa aatgagaatt atcaacaaca aaaattgacg catgaagaaa tcagacaatt
     2821 tcagcaattt tctgaaattt cagataaaga aattgaagat atttcagatt tgacttatga
     2881 attagcaata gtcgctttga aggttattga ctaaataatt attcttgcat ttttcctgtt
     2941 tattggaaaa atataccagg tatggcattt gaaaaaagaa aaatcaaaaa ttacgcgacg
     3001 aaaatggaac ttttcggttt ttaggaaata aaaaccgctg taaacatttg atttacagcg
     3061 gttacattgt tatataagtg accccgttgg gattctaacc caagaccttc agaaccggaa
     3121 tctgacgttc tatacagctg aactacggag cctttgttaa atatacagtg caaagataaa
     3181 taaattatcc cgaactgtaa aaagtccggg ataaaaagca cggttagttt ctcctttatt
     3241 tttggttttt aagcatctga tcagtctctg cgccccatga aaatcatgat gtaatagaac
     3301 agagttgcca tggaactcag agcagctacc acataggtat atgcagccga tttgagtgca
     3361 ccggtagcag catcacgcgt ttcgtaattg gttatcccgg cattactcaa ccacgctaca
     3421 gcgcgttgac tggcgttaat ttccaccggg agggttatga tgctgaataa agtggtgaga
     3481 ccaaaaagta ttattccaaa aaacaacaaa ctaggaaaga cctgcagtaa taaaattccg
     3541 cccagtaata tccactgaac ccagctggat gcaaaactga caaccggaac cagtttcgaa
     3601 cgcatagtaa gcggagcata cgccgtagca tgctgaacag catgtccgca ttcatgagca
     3661 gccacagctg cagcagccac actatcggaa gcataaaccg cttcgctaag gtttactgtt
     3721 ttgttggcag gattgtaatg atctgttagt tggccggctg tggaaatcac ctgaacatcg
     3781 gtaatgccgt tatcgcggag cattttcaca gctacatccc gaccagtcat tcctgaagga
     3841 agtggaattc tggaatattt cttaaacttg gattgaaaat tggcttgtac aatccagctg
     3901 actaaagcta tcaatccaaa aagcacatac gagtaaatca taattgttat tctttaaaag
     3961 ttattgaata caaaacatca aaattcacgc caaagaaata aactgacaaa atggcagaaa
     4021 aaaaccaact tttcaaattc gaccttcttt ggaacaaaga aatccccagg acagttaagt
     4081 gtttcagtgg taatttttgg aaagaataaa ttttgattgt aaaaaaaatg ccacatgaag
     4141 tttacatgtg gcattgttat gagtttttag tcctgatgcc tgattatggt ttgctcacgg
     4201 tcgggaccga cggacactat tttcaccgga actccaagtt cttcttccag aaagtcgaga
     4261 taagcattga gttcttcggg gaattcatct tcgcttttaa tggatgtaag gtttgttttc
     4321 catccgggaa gttccgcata aacgggttca acgccatcgt tcagatcaaa aggaaaatct
     4381 tcagtttcta cgccatcaat tttataagcc acacaggctt taatggtttc aaaactgtcg
     4441 agtacatcag ctttcatcat aataagctga gttactccat tgatcatgat agcataccgg
     4501 agcgctacca ggtcaatcca tccgcaacgg cgcggacgtc ccgtaaccga tccgaattca
     4561 aaaccgatac gacgcatttc gtcaccatcg tgatcgaaaa gttcggtagg aaatggaccg
     4621 ctgcccacac gagtacaata ggctttaaag ataccatata catcaccgat tttgttgggc
     4681 gccactccca atccggtaca tgcacccgca caaatggtgt tggaagaggt tacgaacgga
     4741 taggaaccga agtcaatatc gagcatggta ccctgcgcgc cttcagccag cacttttttc
     4801 ccgcttttca gggtgtcgtt aataaaatgt tcgctgtcaa taaactgaaa ctctttgagt
     4861 ttttcgatgc cttcgaacca tactttttcg agtgccggca gatcgaattc tatctgataa
     4921 tgcttcagta tatcgagatg cctgtttatg gctgctttat atttttcatc aaaattatga
     4981 agcagatcac ccacacgaag tccgttacgg ctcaccttat cggtataggt tggcccgata
     5041 cctttaccgg ttgtaccgat ttttccacta cccttggccg actcgtaagc catatcgagc
     5101 aaacggtgtg ttggcagaat taagtgagct ttctttgata ttctcagtct gtcagtaagt
     5161 gcatgtcccg atttttcgag cgcttccact tcctccttaa acagggccgg gtcgagaaca
     5221 acaccattac caataacatt gattttatca ccctggaata ttccggaagg aatggagcgt
     5281 aacacgaatt tgcgtccttc gaactcgagg gtatgaccgg cattgggtcc gccctggaaa
     5341 cgactgatga cgtcataaga gggtgttaat acgtcaacaa cttttccttt gccttcgtcg
     5401 ccccactgaa ggcctaacaa tacatctatt ttcatttgtc tgaatttatt tttctgtctt
     5461 ataagctttc cggtccggaa aacaggctgt aaatttattc gattaaagct tttttactcc
     5521 gtttttttgt tgcgcgctac atttgctgca cagcccatac acgtaaagcg tatagcgtga
     5581 aggttcgaac cggggtatct tctttttttg taaaagtttt gatatggtga catcgggtac
     5641 ttcccggatt ttgccgcaac tggtacaaac caggtgatga tgaaagatga tgccaaatgt
     5701 cttttcatat tgtgccatct gttcgccaaa ctgatgtttc acaatcagat tacattcttg
     5761 caacagttcc agagtgttat atacggtagc caaactcacc tgatattctt tcggcatctg
     5821 attatatatc agttcggcat tgaaatgccc ctgatgctta tagatatagt cgagtattgc
     5881 atatctttca cgcgttttcc ggtatttatt tttgaccaga taatcggtaa aagtctgcat
     5941 caattcttca tgtgaatttt cttccttcat tcaagagctg ctatttttag gaaaacaaaa
     6001 gtacgacaat ttgatgagac tttcaattaa aaattcaaaa aagatatttc ctgcgacact
     6061 tcgctgaaaa tatacttttt tgatggatga tgggagtttt tcatacaact gatttcttcg
     6121 taaatcctgt ccgaaagctc tttaccggtt ctgcacaggc gacttaatat caaagcaatt
     6181 gatttataaa gatggtattc ggctgtatca gagatttcaa gtgcccgatg aataagttcg
     6241 tcactctggc tggcttcaaa acttttccag atacgtgccc caagattgaa agcggtaatc
     6301 tgatgccaga gcttatccga ttttgcccag cggatactga gttccactgc aaaaggcagt
     6361 ttggcaaaaa ggttcatact gcattgctct actatttcgg gatgtgtgag tgaaaccagc
     6421 cattcatcag ccatttcgga cgtaaaacta tcgaccggat ggagtagcgt tgccaaaatc
     6481 atggtttcgc gtatctgcaa tgcccataag cgctgagcca gatccttgct ctgatcataa
     6541 ccggatgcta tttgtttcag ccgcgggata tccaccccga agttttgctt ataactgatg
     6601 ccgctttcct tcatcttatc cgagaccaca ccgttcatcg acagccttat tttttttcgg
     6661 atttctttta ttgtattgtc tatttgctca ttctgaatat aatacttcat acactttatt
     6721 tttttgtaaa catttttctt ttaaaaactt gcacagtttg caattcaatg ctatctttgc
     6781 actcgcaaaa gcatggtggt tgtagctcag ttggtcagag cgtcggattg tggttccgaa
     6841 agtcgcgggt tcgagccccg tctcccaccc tacaatagaa aaggaaattc agcaatgagt
     6901 ttcctttttt tatcatgaca ccatattctt tattctggct cacctatagg tatagtggta
     6961 gtacttggtc ttcgataatt atttacaaat ttatcattta tttctttata tacttctatc
     7021 ttttttattt ttgcattttt atagaaatta tagaattttg gttcccaata atcatcataa
     7081 tctccaacta attttccttc ttcttcataa cttaatggag ttgtatcgtc tatatatata
     7141 tatatataat agatataatc gtttactcca tctttgtaaa ttacgtctaa acataatgat
     7201 tctattttac ctaataaatt tttatttaca acataaggac tgtgctcgat gctcgtatat
     7261 ttagagccac tcaactgaag ttcactctga tcagcccaaa aaccaattgt tttaccgatg
     7321 taagccgcag cattgtcctg ataatttttt tcgatatacc gcaaaggatc gctgccacaa
     7381 tttaccagtg tagtataag
//
LOCUS       DAOPYB010000152         7345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12279_length_7345_cov_9.207682, whole genome shotgun sequence.
ACCESSION   DAOPYB010000152 DAOPYB010000000
VERSION     DAOPYB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12279_length_7345_cov_9.207682"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /gene="tsaD"
                     /locus_tag="PKH58_10400"
     CDS             <1..875
                     /gene="tsaD"
                     /locus_tag="PKH58_10400"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNX89483.1"
                     /translation="NIIPVIDQALHEANIQKNQLSAIAFTRGPGLLGSLIVGTSVAKS
                     LGLALNIPIIEVDHIQAHMLAHFIVEQNHTKQTPQFPFLCLLVSGGHTQIIKVKDFYT
                     FQTIANTIDDAAGEAFDKSAKILGLPYPGGQMIDKNAAAGDPLKYKFAEPQVKDDEFS
                     FSGLKTSFLYFIRDNLKSNPDFIDQNLADLCASIQHTIITILVKKLIKASTLTGIRQI
                     AISGGVSANSALRDMLLSLAEQQRWKVFLPKPVFCTDNAAMVAVAGYYKYLNKDFTGQ
                     DSIPYTRYIVPEGK"
     gene            1232..>7345
                     /locus_tag="PKH58_10405"
     CDS             1232..>7345
                     /locus_tag="PKH58_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89484.1"
                     /translation="MKKIIFIFATAAILLGLSRNARAQNVGVSDVAFTPQSLLHVYNS
                     SAGRLLQLTNTNTGNTATDGFNLSIDGSLNIVFNQYENAKMSFYTNNSERITILGNGL
                     VGIWNNNPTQAKLNVLSANSSQQGIYSEHNSAATGTAYSAIQGVYSGTGYTAAQGNIA
                     YHASTNKTYSLYSTGGDYGAFIQNKVVIGNVHPDVAKNIFDLEVQNTAAGAGNPATIA
                     IRQTNSLTTNGDILGLLNFADNSQANPQAQIRVFRDATAGGLGSSDYPTALTFLTTPD
                     GSSIMSERLRVTNDGNIGIGTISPAKKLDVAGGDIRTTNQLISTVATGTAPLAVSSTT
                     KVTNLNADLLDDLNTGNGNGNIPISNGTVNTNLNADLLDGLHAASFAQAANAYIQNGN
                     SFGALAVIGTNDAFDLAFETNSSEKMRILSGGNVGIGTTNPAYKLDVTGTGRISDNSF
                     IGPIPPAYPFKSAFTWQSDNTGYYAGIGYQKTDGTNQKLQMWWIDNVAETNINSVLNV
                     GTPGHTFDGVAPNAGRTIGASIASAGVSYFNGGNVGIGTASPGQKLDVSGGSIRTTNQ
                     LISTLANGTAPLVVTSQTLNSNLNADMLDGYHYNNLPYLTNANISGTTNYVSKFTSNT
                     AIGISQIFDNGTSVGINNASPAVRLDIAEAQQTEFYGTDMANPIANMIIRGTGTSRTL
                     GTGSAIEFVVPANTDGSNPWAQARILGTPDNSSNGNAEGALYLQTRANYNPGPGGSWN
                     WRTNMVLRASGNVGIGTTSPGQKLDVVGGNVRTTNQLISTVVTGTAPLAVSSTTKVTN
                     LNADLLDDLNTGNGNGNIPISNGTVNTNLNADMLDGQHYSASWVNPANMVTGSGTATR
                     VAFWDGTYTLNSNANLYWDNTNSRLGIGTASPAEKVNILVGSTGADAVAGLRIGGPSN
                     YQSLELGIVEGGSYEAMIRTYGNDLRLYAGHWRTTGATASEDHAIKFYTSKSGSTNWN
                     TPKMILNQEGNVGIGTTVPAYKLDVNGNIRSGYANNSALLVQASGTASSQAAIAIQQL
                     TAEGWTAIFADFEPNAGWGFYHDNPSNYFIVTSEAPAGNLGSTTVTNRSGAARTAYYK
                     HIFDQNDGDMFVGGNVGIGTTSPVAKLDVSGGSIRTTNQFISTLANGTAPLAVTSTTQ
                     VNNLNADLLDGNHLSSLQFYQSSRDFPNGTLIQTNIWYADPQGEPWLLEIEGNSYGNL
                     VPFDIKVQGYIYSNTLINYGGISNGTNISGLVAFNYNNYLCFWFPNQAYWQGYSVFVN
                     DSYAGIKENRLVSITHEIKPAGVTKEVALSSSIRQSWHSGNFTPSDYAPVSGSNNYII
                     NGTSQQSANFNISGTGTVGTLAMGNNWSLYGSAWPGSGGFLYTGNDVTFGFGSSGGQA
                     SVQVDGAFIQAEAGKTNTFAGTSIFNNGVTINQDNSIDAVSGSNIGSLHMTHPTSGGL
                     SAITFRSTVNWPSDGGYLAYYDHNSSYAYWGSGNENSALVLGTTNDGQTSASDVVVLK
                     SQAAAIIDAPSLLIPSGNVGIGTTTPQRKLHIAGNDMGTNNVINNDGNDRPGIAVRGY
                     YPEIDIISNVYNQGGNGSHGATLRFVGYDNASNWKHWVIGTSGSNSVNLDFGYAVNQS
                     NPHCGIDNYSGSTFMRITSAGNVGIGTTSPSFKLHVPSGYIGTDYINTTDNSVGSGVS
                     GIMVKAGDNYHRTANAAAVLSFLGVSAPTGDNLGNHLATTTLSMNGNLISAAGKITFS
                     GVGGNSGQGNDSYAIYQEAGAWSFPYPDLCIGYHTGIKLGAHYSYNGIRFYNNSDFAT
                     QTFSVGDGDNNVRVYYDMATVGDIFSNQNYGKGLVGLYSSTRYQNVFAMGSAYRLAAD
                     GSTPGNLYGLAWTHTNVGGQSKPGLSHQLLVMENGSTSVALGTGIWTKGNLSFNDANP
                     YISASSYIVMPGGLYVSGGTLYVTNQAQMRGGIHNDNAGDLTIAGGTNGTTYFSGPIS
                     GMSGYYPSNNMIRLTPNLHLNATAGNAVILNWDNGTTGTSQTLRIGDGAGSDVFNVLA
                     NGNTGIGTTSPGYKLHINGGTT"
BASE COUNT         2228 a         1483 c         1558 g         2076 t
ORIGIN      
        1 taaatattat tcccgttatt gaccaggcac tccacgaagc aaatatacaa aaaaatcaac
       61 tttctgcaat tgcatttaca cgaggtcccg gtttgctggg ttcacttatt gtcggaactt
      121 ctgtggcaaa atcattgggg cttgctttga atatcccaat cattgaggta gatcatattc
      181 aggctcatat gcttgcacat tttattgtag agcagaacca cacgaaacaa acgcctcagt
      241 tcccattcct gtgcctgctt gtttcggggg gacacaccca aataattaaa gtaaaagatt
      301 tttatacgtt ccaaaccatc gcaaacacca ttgacgatgc cgccggtgaa gcttttgata
      361 aaagtgcaaa aattttaggc cttccatacc ccggcgggca aatgattgac aaaaacgcgg
      421 ctgccggcga tcctttaaaa tataaattcg ctgagccgca ggttaaagat gacgaattca
      481 gcttcagtgg gctcaaaacc tcttttttat attttatccg tgacaacctg aaaagtaatc
      541 ctgattttat tgatcaaaac cttgctgatc tgtgcgcttc aattcagcat accatcatca
      601 ctattttagt caaaaaactc atcaaagcct ctacactgac cggtataaga cagatagcaa
      661 tttcgggtgg tgtttcggca aattctgcct tgagagatat gctgctgagc ctggcagaac
      721 aacaacggtg gaaagtcttt cttcctaaac ctgtattttg caccgacaac gccgcgatgg
      781 ttgctgtagc tggttattat aaatacctga acaaggattt taccggacag gacagcattc
      841 cctatacccg gtatatcgtg ccggaaggta aatgactcca agattcataa acaccacacc
      901 aatttttcac gaccggtacc ccaaaaaaca taataaaaaa tatttgacag gataattcat
      961 gaatatctgc ctgcatgaca atatgtttac cgcttttatg ggtgttagtg cctgataata
     1021 ttttccatat ttcattcatc acaaaatttg tctgtttttt cttgatttat acattaattt
     1081 tcattaattt tacaatgaaa attatttgtc tgtgatttca cctgaattct acatttttta
     1141 cggaataata acacaataag cacttataat ttgcagaaaa tattatattt gcactttgaa
     1201 aatatttgta atttttttaa tacaataaaa gatgaaaaaa ataattttta tcttcgctac
     1261 cgccgcgatc ctgctgggtt tatcacgcaa tgcaagggca caaaacgtag gtgtatccga
     1321 tgtggctttc actccgcaga gtttgcttca tgtatataac agttcagccg ggcgactttt
     1381 acagctaaca aacacaaaca cgggcaatac ggcaacagac gggtttaacc tcagtattga
     1441 cggctcattg aatattgttt ttaatcagta tgaaaatgca aaaatgtcat tttatactaa
     1501 caattccgaa agaattacaa ttttaggcaa tgggttggta ggcatttgga acaacaaccc
     1561 gacacaggca aaacttaatg ttttgtccgc caatagttca cagcagggga tatattctga
     1621 acataacagc gctgccacag gaacagcata tagcgctatc cagggggtat atagtggcac
     1681 cgggtatact gctgcccaag gcaatattgc ctatcatgca tcaaccaaca aaacgtattc
     1741 gctgtatagc accggaggtg attatggtgc tttcatccag aataaagttg tgataggaaa
     1801 tgttcatccg gatgtcgcta aaaatatttt tgatctggaa gttcaaaaca ccgctgccgg
     1861 tgcaggaaat ccagctacaa tagcaataag acaaacaaat tcattaacca ctaacggaga
     1921 tattttagga ttattgaatt ttgccgataa ctctcaagcc aacccacaag cacaaatcag
     1981 agtattcaga gatgcaactg ctggtgggct gggttcatcg gattatccta cagcgctaac
     2041 ttttttaact acccctgatg gttcgtcaat aatgtcggaa aggctcagag ttacgaatga
     2101 cgggaatatc ggtatcggta caatttctcc agcgaaaaaa cttgatgttg caggtggtga
     2161 tattcgtacc accaatcaat taatttcaac tgttgcaaca ggcacggctc ctttagctgt
     2221 aagttcaacc actaaagtaa caaatttaaa cgccgacctg cttgatgatt taaataccgg
     2281 gaatggcaat ggaaatattc caatctctaa tggaactgta aacacaaatc tcaatgcaga
     2341 tttgttggat ggattgcatg ccgcatcgtt cgcgcaagca gcaaatgctt atattcagaa
     2401 tggcaactca tttggtgcat tggctgtaat cggaacgaat gatgcttttg atctggcttt
     2461 tgaaaccaac agttccgaga aaatgaggat attatcgggt ggcaatgtgg gtatcggaac
     2521 aaccaatcct gcttacaaat tagatgtaac cggcactggc cgtatttctg ataattcttt
     2581 catagggcca atcccacctg catacccatt caagagcgca ttcacctggc aatcggataa
     2641 tacaggttat tatgcaggca tcggatacca aaaaactgat ggtactaacc aaaaattgca
     2701 aatgtggtgg atagacaatg ttgccgaaac taatatcaat tccgtattaa atgtcggaac
     2761 tccagggcat acatttgatg gcgtggcacc aaatgcagga cggaccatcg gtgcaagcat
     2821 tgcttcagca ggggtttcct attttaatgg cggaaatgta ggtataggaa cagcctcccc
     2881 cggacagaaa cttgatgtat caggcggttc catacgtacc accaatcaat taatttcaac
     2941 tctggcaaat ggtactgccc cgcttgtcgt tacttcacaa actctaaaca gcaaccttaa
     3001 tgcagatatg ctcgacggat accattataa caacctgccc tacctgacca atgcgaatat
     3061 ttcaggaacc acaaactatg tttcaaagtt cacttctaat actgctattg gcatctccca
     3121 aatatttgat aacgggactt ctgtagggat taataatgca agccccgctg tacgcctcga
     3181 catcgccgaa gcccaacaaa cagaatttta tggaacagat atggcaaatc ccatagcaaa
     3241 tatgataata agaggtaccg gtacatcaag aactttgggt acaggaagcg ctatagaatt
     3301 tgttgtacct gccaataccg atggttccaa cccttgggca caggctagaa tattaggcac
     3361 tccggataat tcgagtaatg gtaatgccga aggggcatta tatttacaaa caagggctaa
     3421 ttataatccc ggcccaggtg gttcatggaa ctggagaaca aatatggtgc tcagggcttc
     3481 cgggaatgtt ggcataggaa ccacatcccc cggtcaaaaa cttgatgttg tcggtggtaa
     3541 tgtacgtacc acaaatcagt tgatttcaac tgttgtaaca ggcacagctc ctttagctgt
     3601 aagttcaacc actaaagtaa caaatttaaa cgccgacctg cttgatgatt taaataccgg
     3661 gaatggaaat ggaaatattc caatctctaa tggaactgta aacacaaatc tcaatgcaga
     3721 tatgctggat gggcagcatt attccgcgtc ctgggtaaat cccgctaata tggtaacagg
     3781 atccggaact gctaccaggg tggctttctg ggatggtact tataccctaa acagtaacgc
     3841 caacttatac tgggataata cgaatagccg gctgggtatt ggaactgcaa gccctgctga
     3901 aaaggtaaat attcttgttg gatctaccgg tgcggatgcc gttgcaggtc ttagaattgg
     3961 aggcccttca aattatcagt ctttggaatt gggtattgtt gaaggtggta gttatgaggc
     4021 gatgattaga acttatggaa atgatttacg tctttatgct ggtcattgga gaactaccgg
     4081 agctactgct agtgaagatc atgcaataaa attctatacc agcaaaagtg gatctactaa
     4141 ctggaatact cctaaaatga ttctaaatca ggaaggtaat gtcggtatcg gaacaacagt
     4201 acctgcttat aaattagatg taaatggcaa tatcaggtct ggttatgcaa acaatagtgc
     4261 acttcttgtt caggctagtg gaactgcttc cagtcaggct gcaatagcta ttcaacagct
     4321 taccgcagaa ggctggaccg caattttcgc cgattttgag ccaaatgccg gttggggatt
     4381 ttatcacgat aatccaagta attactttat agttacatcc gaagctccag caggtaatct
     4441 gggaagtaca acagttacaa accgcagtgg agctgcccga acagcatatt ataaacatat
     4501 ttttgaccag aacgacggtg atatgtttgt tggcggcaat gtcggtatcg gaacaaccag
     4561 ccctgttgca aaacttgatg tatcaggggg ttctatacgt accacaaatc agttcatttc
     4621 aactcttgca aatggtactg ctcccttagc ggttacttca acaactcagg taaataatct
     4681 gaatgccgat ttacttgatg gcaatcactt gtcatcacta cagttttatc agtcgagcag
     4741 ggattttcca aacggcactc ttattcagac aaatatatgg tacgctgatc cgcagggtga
     4801 accctggttg ttagagatag aaggaaattc atacggcaat ttagtaccgt ttgatataaa
     4861 agtacaaggt tacatttata gtaataccct tattaattat gggggcattt caaatgggac
     4921 aaatatttca ggattagtag catttaatta taataattat ttgtgctttt ggtttcctaa
     4981 tcaagcttat tggcagggat acagtgtttt tgtaaacgac agttatgccg gtataaaaga
     5041 gaacagatta gtttcaataa cccatgaaat aaaaccagcc ggagttacta aagaagttgc
     5101 cttgtcatcg tcaataagac aatcatggca ctctggaaat tttacgcctt ctgattatgc
     5161 acctgtttca ggtagcaata attatataat taacggaaca agtcaacaat cagcaaactt
     5221 taatatttct ggcaccggca cagtaggtac gctggccatg ggcaacaact ggtcgcttta
     5281 cggatccgcg tggccaggtt ctggtggttt tttatatacc ggaaacgatg tcacctttgg
     5341 tttcggatca agcggtggtc aggcatcagt gcaggttgac ggagctttca tacaagctga
     5401 agcaggtaag acaaatactt ttgcaggcac ttcaatattc aataacgggg tcacaataaa
     5461 tcaagataat agtattgatg ctgtaagtgg aagcaatatt ggcagcttgc acatgactca
     5521 tcccaccagc ggaggtttaa gtgcgatcac gtttaggtct actgtaaact ggcctagtga
     5581 tggcggatat cttgcatatt atgaccataa ttcctcttat gcttattggg gaagtggtaa
     5641 tgaaaatagt gcgcttgttt taggcaccac taatgatgga caaacttctg cttccgatgt
     5701 tgtggttctc aaaagtcagg ctgctgcaat aatagatgct ccaagtttac ttatcccttc
     5761 cggtaatgtc ggtatcggaa ctactactcc tcagcgaaaa ttgcatatag ccggtaacga
     5821 tatgggaact aataatgtaa ttaataacga tggaaatgat cgtccaggta ttgctgttag
     5881 aggctattat cctgaaattg atattatctc taatgtatat aatcaaggag ggaatggtag
     5941 tcatggggct acattaaggt ttgttggtta tgataatgct agtaattgga aacattgggt
     6001 aatcggaaca tctggaagca attcagtaaa tctcgatttc ggttatgcag ttaaccaatc
     6061 aaatcctcat tgcggtattg ataattattc tggttctact tttatgagaa ttacaagtgc
     6121 tggcaatgtc ggtatcggaa ctacaagccc ttcttttaaa cttcatgttc catcagggta
     6181 cattgggaca gattatatca atactaccga taactcagtt gggtcaggtg tttcaggaat
     6241 aatggtcaaa gccggggata attatcacag aactgcaaat gctgctgcgg tattatcttt
     6301 tttgggtgtt tcggctccca ccggcgacaa tctggggaat catttggcaa caacaacatt
     6361 aagtatgaat ggaaatctca tatctgctgc agggaaaatt actttcagtg gcgttggcgg
     6421 taactccgga cagggaaatg attcttacgc aatttatcag gaagccggag catggagctt
     6481 cccctatccc gacctctgta taggctatca tacaggcatt aaattaggtg ctcattatag
     6541 ctataacggg atacggtttt ataataattc tgacttcgca acacaaacat tttcggtggg
     6601 tgatggtgat aataatgtac gagtatatta tgatatggct actgttggtg atatattttc
     6661 aaatcagaac tacggaaaag gacttgtagg cctttattcc tctaccaggt accaaaacgt
     6721 ttttgccatg ggaagtgcct atcgtctggc tgctgatggc tctaccccgg gaaaccttta
     6781 cggcctcgcc tggacacata caaatgtggg agggcaatca aaacccggat tatcgcatca
     6841 gcttttggtg atggaaaatg gaagtactag tgtagccctc ggaaccggta tatggacaaa
     6901 gggtaattta agctttaacg atgccaatcc atatatcagt gcatcttctt atattgtcat
     6961 gccaggcggc ttatatgtta gtggcggaac tttatatgtt actaaccagg ctcaaatgag
     7021 aggaggcatt cacaatgaca atgctggtga tttgacaatt gcaggcggta caaatggaac
     7081 aacatatttt agtggtccta tttcaggtat gagcgggtat tatccgtcaa ataatatgat
     7141 acgtttaaca cccaacctac accttaatgc aacggcaggc aacgccgtga tactaaactg
     7201 ggacaacgga accacgggga cttcacaaac tttaaggatc ggcgatggag cagggtcgga
     7261 tgtttttaat gttttagcca atggcaatac tggcatcgga acaaccagcc cagggtataa
     7321 gctgcatatt aatggcggca ctacc
//
LOCUS       DAOPYB010000153         7344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12282_length_7344_cov_9.959528, whole genome shotgun sequence.
ACCESSION   DAOPYB010000153 DAOPYB010000000
VERSION     DAOPYB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12282_length_7344_cov_9.959528"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..589)
                     /locus_tag="PKH58_10410"
     CDS             complement(8..589)
                     /locus_tag="PKH58_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNX89485.1"
                     /translation="MNAEILTAVALGIGLSASTGFRVFLPMLVASVAAHFGILPLSDS
                     FLWLGSWPAIISFGTATVVEVAAYYIPFVDNLLDTITTPLAVGAGTLLMTSVLPVDND
                     LLKWIAGFVIGGGAAATIQSGSVVSRLTSSKFTAGTGNPVVSTGENVAALGTSILTLF
                     IPVIIAAAFILLSVFILYQLFRWRKSKREHKVQ"
     gene            complement(901..3327)
                     /gene="lon"
                     /locus_tag="PKH58_10415"
     CDS             complement(901..3327)
                     /gene="lon"
                     /locus_tag="PKH58_10415"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNX89486.1"
                     /translation="MMKRYDNLFSNDMMGSSEVFPIISLEDNPTDINLSSGDVLPILP
                     LRNMVIYPGVLVPVSVARPKSLRLIRQAQDNDGLIGACTQMDKKVEDPTLEQLYKTGV
                     VAQIVRLLEMPDNTTTVILEGKKRFQLGEQVSTKPYLKAVVHLLDEALPDTTDKLFIA
                     LTSSIRDLAIKIIENSGIIPQEMAFAIRNIENPVFLINYICSNFGLDVNEKQRLLEID
                     EVMERGYQLLSLLNKESQLLEIKMTIQNKAKEGIDQQQREYFLQQQMKTIQNELGEGS
                     PEQDVEDFKTRAKSKKWSDNVAKIFDKELKKLERMSQHSPEYTTQLNYVETMLDLPWN
                     EYTKDNFNLKNAKKVLDRDHFGMDTVKERILEHLAVLKLKGDMKSPIICLYGPPGVGK
                     TSLGKSIATSLNRKYIRMSLGGLHDEAEIRGHRRTYIGAMPGRIMQNIKKAESANPVF
                     VLDEIDKVNADYKGDPSSALLEVLDPEQNTAFHDNYLDIDFDLSKVLFIATANNLNTI
                     PRPLLDRMELIEVSGYTQEEKVEIAVRHLVPRELENHGLKAEQFVLTKPLTNQLIDQY
                     TRESGVRELDRKIAALMRKVAKNVAMGTKYNVELTSEDLKSYLGVPRYSRDKYEGNSY
                     AGVVTGLAWTSVGGEILFIESSLSPSKSPALTLTGNLGDVMKESAMLALQYIKAHTNE
                     LGIDQKMIDENSVHVHVPEGAIPKDGPSAGITMTTALVSAFTRRKVRSNTAMTGEITL
                     RGKVLPVGGIKEKILAAKRAGITDIVICEENRKDIEEIKNTYLKGLKFHYVTDIMEVI
                     NFALLEEKVK"
     gene            3789..5519
                     /locus_tag="PKH58_10420"
     CDS             3789..5519
                     /locus_tag="PKH58_10420"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNX89487.1"
                     /translation="MKKSHLFILPVILSLSVLFCQNNSDMTTKRNTSIPDSASFSLAM
                     LDAVNASDSTNFSLLQQVYLPRAYNSFWFNNTTKFNERILLLDSFLQHADEHGLTPGR
                     FGAQKILDLLSKTDTTNIDYQHLAQLEIQATQSYYSYCSGLQYGCYNPVKLYPDDYFI
                     APRQPDSAFIRQIFSNPDSLRYYLRSVQPVSGEYKILQAELRKIRKLSDSTFRHVPLM
                     PADEQIKLNRKHPSVPLIARRLMISGELPFRPKWDSIYTRFDEHLLKALNQFRKQNNL
                     IQDNEIGNPTISALNINFKKRSQIVAINLERLRWKPKNHISNKFVEVNVANMMLRTMR
                     GDTVTNTMKVCVGQPPKHKTPLLYGKMYEVVLNPTWTVPSSIIINEISRKMISDPSYI
                     SRNRMKVYKNREAVSEYSVPWSSISKKYQPYVIVQDAGEGNSLGRIKFNFSNPFNVYL
                     HDTNAKSVFQRHYRAVSHGCVRVERPLELAYFCMNDIDTTSKKQVSKRNLLKDQILYS
                     IDRQPLTRQGREYFETEGKGKKMSRIELNPGVTVLLDYKTCFRGKDGSVQYCYDHYRM
                     DSLLINKLFP"
     gene            complement(5516..6007)
                     /locus_tag="PKH58_10425"
     CDS             complement(5516..6007)
                     /locus_tag="PKH58_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNX89488.1"
                     /translation="MRTSFITEISEIEEILEGLHTCFVGMNEEDGSPYVIPMNFGYAD
                     GNIILHSGPHGKHLDLLARDNRVCVTFCTENNKLMCQHPDVACSYSVASKSVLFRGRI
                     EFVEDIAEKERLIKEFMKHYISREFTISAPAIRNVKVWLLRPEKITAKAFGQNFRNTK
                     SPV"
BASE COUNT         2071 a         1649 c         1468 g         2156 t
ORIGIN      
        1 gttacgctta ctgaactttg tgttctcttt ttgattttcg ccagcggaaa agttgataca
       61 aaataaacac cgacagcaaa ataaaagcag ccgcaatgat aacgggaata aagagtgtga
      121 ggatggaagt tcccaaagca gccacatttt cgccggtgga tactaccgga tttccggtgc
      181 cggcggtaaa cttactggaa gtgagccgcg aaacaacact tccgctctga atagtagcag
      241 cggctccccc gccgattaca aaaccggcaa tccatttcag caaatcatta tcgacaggca
      301 gcaccgaagt cattaacagc gttcctgctc ccacagccag cggtgtggtg atggtatcaa
      361 gcagattatc gacaaaagga atataatatg ctgccacttc gactactgta gcagtaccaa
      421 aactgatgat ggcaggccag cttcccagcc acagaaaact gtcggataag ggtaaaatac
      481 caaaatgagc ggcaacactt gccaccagca tcggaagaaa aacacggaaa cccgtgctgg
      541 cactcagacc aatccccaac gctactgctg ttagtatttc ggcattcata cagcgcaaaa
      601 atacaactat tttttaaatg gcaacaggta aagtcctgat ttttttgaga atagtgatta
      661 gtgactgata actggtgatt agtggttagt gattagtgaa ggcatcattc ataatttttc
      721 atttgtagtt cattgtttat agttctatta ttcaccaatg ataagttatt cattttcaaa
      781 ttggcacatc ttcaaattta cacattggta cattagcaca ttgtcaattc tcttttaaac
      841 gccaaacagg ttcatacaat atatgtacaa acctgtctgt agttggtttt ctgttgggaa
      901 ttattttacc ttttcctcca gcagggcaaa attgattacc tccatgatgt cggttacata
      961 atggaatttc agccctttga ggtaggtgtt ttttatttct tcgatgtcct tgcggttttc
     1021 ctcgcagata acgatatcgg ttattcccgc ccgtttggca gccagaattt tctccttgat
     1081 acctcccaca ggcagcactt tgccacgcag ggtaatttca ccggtcatgg ctgtattgct
     1141 gcgcactttg cggcgggtga acgcggatac cagggcagta gtcattgtga tgcctgccga
     1201 tggtccgtcc ttggggatgg ctccttcggg cacatgcaca tgtacgctgt tttcatcaat
     1261 cattttctgg tcgataccga gttcgttcgt gtgagctttg atatactgta acgcgagcat
     1321 agccgattct ttcatcacat cacccagatt accggtgagt gtaagtgccg gtgatttgct
     1381 ggggctcaga ctactttcga taaacagaat ttcaccgccc acactggtcc aggccagtcc
     1441 ggtaacgact cccgcatagc tgttgccttc gtatttatcg cggctataac gaggcactcc
     1501 caggtaggat ttgagatcct cggaagtgag ttctacattg tatttcgtac ccatcgccac
     1561 attcttagcc actttacgca tcagcgcggc aatcttgcgg tcgagttctc gcactcccga
     1621 ctcacgggtg tactggtcga tcagttgatt ggtcagcggt tttgtcagta caaattgttc
     1681 ggccttcagt ccatggtttt ccagttccct gggcaccaga tgacgaacgg caatttccac
     1741 tttttcttcc tgcgtataac cgctcacctc aatcagttcc atacggtcga gcagcggccg
     1801 gggaatggta ttcaggttat tggctgtagc aataaagagc actttcgaga ggtcgaaatc
     1861 aatatcgagg taattatcgt gaaacgcggt attttgttca ggatcgagca cttcgagcag
     1921 agccgacgaa ggatcgccct tatagtcggc gttaacctta tcaatttcat caagcacgaa
     1981 caccggattg gccgattctg ctttcttgat gttttgcatg atacgtcccg gcatggctcc
     2041 tatataggta cgccggtgtc cgcgtatttc agcttcgtca tgcagtccac ccagtgacat
     2101 tcggatgtat ttccggttga gggatgtggc aatggatttt ccgagagagg ttttacccac
     2161 ccccggaggt ccgtataggc aaataatggg tgacttcata tcgcctttca gcttaagaac
     2221 cgccaggtgt tcgaggatac gctccttcac cgtgtccatc ccgaaatgat ccctgtcgag
     2281 aaccttttta gcattcttca ggttgaaatt atctttggta tattcgttcc acggcagatc
     2341 gagcatcgtt tccacgtagt tgagctgcgt ggtatattcc ggcgaatgct ggctcatacg
     2401 ttccagtttc ttcagttcct tatcaaagat cttggcaaca ttatccgacc attttttgga
     2461 tttagcccgt gttttgaaat cttccacatc ctgttccggt gatccttccc ccagttcatt
     2521 ctgaatggtt ttcatctgct gctgcaggaa atattcacgt tgctgctgat cgatgccttc
     2581 ctttgcttta ttctggatgg tcatttttat ttcgagcagc tgtgattctt tgttgagcaa
     2641 actcagcaac tgataccccc gttccatgac ctcatcaatt tcgagcaggc gctgtttttc
     2701 gttcacatcc agcccgaagt tggaacagat atagttaatc aggaacaccg gattttcgat
     2761 attccggatt gcaaaagcca tttcctgcgg aataattcct gagttttcga tgattttgat
     2821 agccaaatcg cgtatggacg aagtgagggc gataaaaagt ttgtcggtgg tatcgggaag
     2881 cgcttcatcc agaagatgaa cgactgcttt caaatagggt ttcgtggaaa cctgttcgcc
     2941 cagttgaaaa cgtttttttc cttcgagaat aaccgtagtg gtattgtcgg gcatttcgag
     3001 taacctgaca atttgagcga ccacccctgt tttgtaaagc tgttcgaggg taggatcctc
     3061 taccttctta tccatctggg tgcaggcgcc tattaatccg tcgttatcct gcgcctgacg
     3121 aatcagccgc aacgatttgg ggcgtgccac tgaaaccggc accagtacac ccggatatat
     3181 caccatatta cgcagcggga gtataggcaa tacatcaccc gaactgagat taatatcggt
     3241 aggattgtcc tcgagcgaaa tgatcgggaa aacctccgat gaacccatca tatcatttga
     3301 aaataaattg tcgtatcttt tcatcatctg tctttatgtc cttattacgc tatttatgtc
     3361 acattttcaa tttatttttc tgccattttg tcagtcccga gaatcgtcgt ttccggaaaa
     3421 taaatcatgc caacagcgta acaagttata tatctttaat ttatatttaa tttggctttg
     3481 actgcataat attaattcaa tagttatgcc aaagatttga aataaacttc tgtatataaa
     3541 gaaattcata cgggaatgca aatctaattc aaaaaagcca gccgttgatt gaatacaaca
     3601 gaaaatcttc ggcaaacaaa ttcgttaaaa agataaacct gctttgagtc tgcttaataa
     3661 cgtgtgaatt tagcaggtaa atgcaaattg agtgaaaaac cgtttggtca tttttcaaaa
     3721 tttttatata cctttgtatt gttactgctc tttttttcag atatttaatt caacaaccgt
     3781 ttttctgtat gaaaaaaagc catctgttta ttttacccgt cattctttcc ttatccgtcc
     3841 tgttttgcca gaataattcc gacatgacta caaaaaggaa taccagcata ccggacagcg
     3901 catccttcag cctggccatg ctcgatgcgg tcaatgcttc tgattcaaca aacttttctt
     3961 tgcttcagca ggtttatctg ccgagagcat acaattcgtt ctggtttaac aataccacga
     4021 aatttaacga acgtattctt ctgctcgatt cattcctgca gcacgcggat gaacacggat
     4081 taacacccgg acgattcgga gcacagaaaa tactcgacct gctttcaaaa acagatacca
     4141 cgaatattga ctaccagcat ctcgctcagc tggaaattca ggccacccaa agttactaca
     4201 gctactgctc gggattgcaa tacggctgtt acaatcccgt aaaactttac cctgacgatt
     4261 acttcattgc tccccggcaa ccggacagcg cctttatccg gcagatcttc tccaatccgg
     4321 attcactgcg atactacctg cgcagcgtgc aaccggtctc tggcgaatac aagattctac
     4381 aggcagaact tcggaagatt cgtaaactca gcgattcgac attccggcat gtcccgctga
     4441 tgcccgccga tgagcaaatc aaactgaaca ggaaacaccc ttcggtaccg ctcatagcca
     4501 ggcggctgat gattagcggc gagttaccct tccgtccgaa atgggacagc atatataccc
     4561 gttttgacga acacctgctg aaagccctga atcagttccg aaaacaaaac aacctgatac
     4621 aggataacga aataggcaat ccgaccatca gtgcactgaa tataaatttc aaaaaacgca
     4681 gtcagattgt tgccatcaac cttgagcgtt tacgctggaa acctaaaaac cacatcagca
     4741 ataaattcgt ggaagtcaat gttgccaaca tgatgctgcg gactatgcgc ggcgatacgg
     4801 tgacgaacac catgaaagtg tgtgtgggtc agccgcccaa acataaaact cccctgcttt
     4861 acggaaaaat gtatgaagta gtgctcaatc cgacatggac agtaccaagc agtatcatta
     4921 tcaatgaaat cagccggaaa atgatcagcg acccgtctta catcagccgc aaccggatga
     4981 aagtgtacaa aaaccgtgag gcggtgagtg aatattcggt accttggagc agcatcagta
     5041 aaaagtatca gccctatgtg attgtgcagg atgccggaga aggcaattca ctggggagga
     5101 taaaatttaa tttttcaaat ccgttcaacg tgtatctgca tgacaccaat gccaaaagcg
     5161 tattccagag acattaccgc gccgtaagtc atggctgcgt gcgtgtggaa agaccgcttg
     5221 aactagccta tttttgcatg aacgacatag atactaccag caaaaagcag gtcagcaaaa
     5281 gaaacctgct taaggatcag atactttact ctatcgacag acaaccactc acacgtcagg
     5341 gacgcgagta cttcgaaacg gagggcaagg gtaaaaaaat gagccggata gaattaaacc
     5401 ccggagtgac agttctgctt gactacaaaa cctgcttcag gggcaaagac ggtagcgtac
     5461 agtattgcta cgaccactac agaatggatt cgctgctgat aaacaagcta ttcccctaga
     5521 ctggtgattt agtattcctg aaattctgac cgaaagcttt cgcggtaatc ttttcgggac
     5581 gcagcagcca gactttcaca ttgcggatag ccggtgctga aatagtgaat tcacgcgaga
     5641 tgtaatgttt cataaattcc ttaatcaatc gctccttctc ggctatatcc tccacaaatt
     5701 ctatccttcc cctgaacaaa acactcttcg atgccacact gtaactgcaa gccacatcgg
     5761 gatgctgaca cataagcttg ttgttctccg tgcagaaagt gacacatacg cggttgtccc
     5821 tggcaagcaa gtccaggtgt tttccgtgcg gtcccgaatg caggattata tttccgtcgg
     5881 catagccaaa attcatcggg atgacataag ggctgccatc ttcttcgttc atgccgacaa
     5941 aacaagtgtg tagtccttcg agaatttctt caatttcact gatttcggta ataaagctgg
     6001 ttctcataag cgatggataa taggatttta tttaaaaaac atcgtttgat atctgattaa
     6061 aataaaaaaa tgcctcttca aaggtataaa agtttttaga agtgacattt taagttaaat
     6121 atcaatatat tcaacaaaaa ataaggtcgt tttatgttct ataacataaa aacgttgtat
     6181 ctttgcactg tgtttttcat ggtattagat ttaaggttaa ttgaagattg ggttgtcggg
     6241 agacaacctt tcttttttta aagaagtctc gtccaaaatc agacctctcg ctaaggaagt
     6301 gacttgcatt gaaaacattt caaattcatc ccgttcatat ttcgttcctt tcgtgaaatt
     6361 agttgctaga atataaacgt ccggcaagac ttactgcttc gtaaaccatt ctcagaacag
     6421 ccaatcacca gtcactaatc acaaatcaat aataacgaat tttcaaattg gcacatcatc
     6481 acattttcaa attggcaaat tgtctcatag gcaaattctc aattctcccc tgacaactga
     6541 tttccggaat ttattttttt ttaggcagaa cttctttcag cgtccgcttt ccctatacaa
     6601 tcaacattgt tactcggcaa aaataatcaa atttgtcctg aaatcttttt ccaaaaagta
     6661 aaaacaggta ttatattgcc attatcatcc acaaactctc atttttaagt taaatatgtt
     6721 taatttcgat aatatttttc acgataatat gttttataac ataaaaatgt tgtatctttg
     6781 cactgtgttt ttcatggtat tagatttaag gttaattgaa gattgggttg tcgggagaca
     6841 acctttcttt ttttaaagcc tctccaaaat cccacggctt caccctaagt ccctctcccg
     6901 gctgagagag acttgaagaa gcaaaacgtt tcaatatcct cccgttaata ttattcgtga
     6961 aactcgttga aaagaaaaat aatttggaaa gacttattga tttgtaaatt attctcagga
     7021 tagtcaatca tcaattacaa attacaagtc atcattcata attcataatt catcaatcat
     7081 caataacgaa ttttaaaatt ttcaaattag tcaagtaact attaaatatt atcaccggaa
     7141 aaggtaaata catctgcagg attttgtttt taagttaatt atggttaatt atatcaatat
     7201 atttcaccat aacatgtttt ataatataaa atgttatatc tttgcactgt gtttttcatg
     7261 gtattagatt taaggttaat tgaagattgg gttgtcggga gacaaccttt ctttttttta
     7321 aagcctctcc aaaatcccac tgct
//
LOCUS       DAOPYB010000154         7311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12352_length_7311_cov_8.893743, whole genome shotgun sequence.
ACCESSION   DAOPYB010000154 DAOPYB010000000
VERSION     DAOPYB010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12352_length_7311_cov_8.893743"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..2800
                     /locus_tag="PKH58_10430"
     CDS             305..2800
                     /locus_tag="PKH58_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89489.1"
                     /translation="MKKKISIFILFILVVFQVWAQSPTITEAAGWLESANLRWSPVAE
                     AQSYNVYFTGGGFTDKKIDTQLIRSYGNYFRADIPGLKAGTYTMKVAPVIAGVEGTAS
                     NSEQITVLPHDRNGFAFHNGRVPGAYKADGTPKDNAVILYITEKNKNSVSMTVTGANA
                     NPCVGVQAILDGFKKGKDTRPLIVRLIGQITDLNNMLNGDLVIENDKNASSYITLEGV
                     GDDAVADGWGLRIKNATNIEVRNIGFMNCNSDEGDNVGLQQNNEYIWVHNCDMFYGNA
                     GSDADQIKGDGALDCKGSTYVTFSYNHFWDSGKSNLLGLSEDTNIGLYISYHHNWYDH
                     SDSRHPRVRFYSAHVYNNYYDGIAKYGVGSTMGSSVFVENNFFRNCKYPMLTSMQGTD
                     IYYDSGGTFSGEDGGTIKAFNNSISGQTRYVPYDAANFPVEFDAIETATREQTISNTI
                     KSKQGANVYNNFDTDASLYVKTFIPDEPEAARNKVIVYAGRVTGGDLKWTFNNSVDDA
                     SYAVNTGLKAALTAYKTKLVAVQGENTSPESTQTLTSSGNHNQTVSVGSAITDIVFTW
                     GGDATDVTVTGLPASGITYVKNPATKTITVSGNPSATVTFAVTTTGTSVTPVSITGTI
                     EVTQSTGGTSFIHNFTTDGKNSTFYTITGNLSTSYGTVSYNELTLTQCLKMETSTNIS
                     FTTQQKSTLTLVYNKTYTGTIKIDGVTYTPSNTDGIVTITSLPIGSHTILKGSGSGYL
                     FYMSIVYENSTDIVNSELTDIKIYPNPVTDKLNIISPNRLHFVEIYTFSGQLIFKVNG
                     NMSQVDISKLSKGNYIVKLVSEAGVVSKLIMKE"
     gene            2808..4430
                     /locus_tag="PKH58_10435"
     CDS             2808..4430
                     /locus_tag="PKH58_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HNX89490.1"
                     /translation="MMKMSYLIVLCSFLILATPARTQISDTWISDQGNGTYRNPVLNA
                     DYSDPDVCRVGDDYFMTASSFNCVPGLPILHSGDLVNWQLIGHALNRLMPEEVYTHSQ
                     HGNGAWAPSIRFHNDEFYIYYPDPDFGIYMLKAKNATGPWSDPVLVKAGKGLIDPCPL
                     WDDDGRVYLVHAYAGSRAGIKSILAVAEMNADGNKIISPSRTVYDGHEVDPTIEGPKF
                     YKRNGYYYIFAPAGGVATGWQVVLRSQNVYGPYERKVVMAQGKTGVNGPHQGAWVETR
                     QGEDWFIHFQDKGACGRVVHLQPMAWKDNGFPVIGVDKDGDGCGEPVLEYKKPKITGN
                     FAIQTPPESDEFNGEFGFQWQWHANPQDWWSFSDVRKGILTLFSVSLPDNSKNLWDVP
                     NLLLQKFPSDKFTTTVKFNFIPDERFDGERAGLVIMGLDYALLSIEKKKEGFSISQNE
                     CLGAEKGNTEIKNETVSAEKSEVFFRVNVTQDAMCFFSYSADGNKYTNIGKPFKAREG
                     KWIGAKVGVFCSRPLKNNDGGKMEIDWFRISN"
     gene            4445..5425
                     /locus_tag="PKH58_10440"
     CDS             4445..5425
                     /locus_tag="PKH58_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX89491.1"
                     /translation="MLRKLTLYILFTGFCALTVAQNKTNSPVPRDTSYTLYSTVIKIR
                     KNYPDARLVSPAIPRDIITYKNIIYKTIPQTPYGKRKLYLNIYRPSKIGRLPVVLLIH
                     GGGWSSGSPLLQQSIAVSLCMKGFAAVAVEYRLSPEAIYPAAVEDLNDALAWVCNNAR
                     TYRFDKNKIAVQGCSAGGQLAALLGMRNKDRLIRAVINIDGISTFMDQTTIDRAENFR
                     KNGGKMPADMQWLGGTMSEKPEVWKDASAVYFVNRHSAPVCFINSSLSRFHNGRDELI
                     RKLKKYNIYSEVHTFENSPHSFWLFDSWHQPTIDYASDFLMKVFAVQKNH"
     gene            5921..6592
                     /locus_tag="PKH58_10445"
     CDS             5921..6592
                     /locus_tag="PKH58_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX89492.1"
                     /translation="MSKKVVESAELENVERALSSSEAFIEKYQKQILIGVGVVVLVVL
                     AVMAFNNFYLKPREVAAENAMYKTQEYFAVDSFKVALDGDGKDVMGFKEIASEYGMTA
                     SGNLANAYTGICYYKLGKYQEAVKYLTQYDAEDDFFKTTIIGLTGDCYAEMGETGKAQ
                     DYYSKAVAEKNELSPIYLKKAGILYETKGQTAEAEKMYLQIKNDYPKSMEAGDIDKYL
                     ARVQK"
     gene            6688..7212
                     /gene="ribH"
                     /locus_tag="PKH58_10450"
     CDS             6688..7212
                     /gene="ribH"
                     /locus_tag="PKH58_10450"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNX89493.1"
                     /translation="MATQYHNLSTYDPDLMPDKAKVAEQKYAIAVAEWNPDITEALLD
                     GAVNTFLENGALIENITIVHVPGTFELSFAAKRFYEEYSQADGDNKIHKYSAIIVLGC
                     VVQGETPHFNYVCQGVTAGITELNLRPDGCPVIFGVLTTNTLQQAKDRAGGMHGNKGD
                     EAAITAIKMANIVW"
BASE COUNT         2245 a         1425 c         1572 g         2069 t
ORIGIN      
        1 tggcaagcaa atcagcgaca atagccaggg tatggtaatt cagaaaatta cttacgacga
       61 tggctctcag gcaacgaaca agaaatttgt caaaaccgaa aaatagacct ttttgattat
      121 atgattgtta gtttaccgga caatgacggg agaatgcttc cgcagtgtcc ggtatatcta
      181 ttcgctattt gagaactttt tgcctacttt attttttttg tggttcatcc gtttatagat
      241 attaacagag tgttcttgta caggcatttt actaaaagtt tattgtaatt taatttttta
      301 tattttgaaa aagaaaatca gtattttcat attatttatc ctggttgttt ttcaggtttg
      361 ggcacaatca ccaaccatta ccgaagccgc cggatggctc gaaagtgcta atctcaggtg
      421 gtcacccgtt gctgaggcac aaagctacaa tgtttatttc acaggtggcg gttttactga
      481 taagaaaatc gacacgcaac tcatccgaag ttacggcaac tacttccgcg ccgatatacc
      541 cggtctgaaa gccggaacat ataccatgaa agttgctccg gtgattgccg gagttgaagg
      601 gacagcctcc aacagcgaac aaataactgt gcttcctcac gaccgaaacg gatttgcatt
      661 tcacaacgga cgtgtgcccg gcgcgtacaa agccgatggc acacccaagg ataatgctgt
      721 gatcctctat ataaccgaaa agaacaaaaa ttcggtttcg atgacagtaa ccggagccaa
      781 tgccaatccc tgtgtgggtg tgcaggcaat tctggatggt ttcaaaaaag ggaaagatac
      841 ccgaccgtta atcgtgcgcc tgataggaca aatcaccgat ctcaataata tgctgaatgg
      901 cgatttagtt attgaaaatg ataaaaacgc atcaagttac ataacccttg aaggagtggg
      961 cgacgatgcg gtagccgatg gctggggact tcgcatcaag aatgcaacca atatcgaagt
     1021 gcgcaatatc ggatttatga actgcaacag cgacgaaggc gacaacgtgg gcttgcagca
     1081 gaacaacgaa tacatctggg tgcacaactg cgacatgttc tatggcaatg caggctccga
     1141 tgctgatcag ataaaaggcg atggtgcact cgattgcaag ggatcaacgt atgtaacttt
     1201 ttcatacaac catttctggg attcgggcaa atcaaacctg cttggattga gcgaagatac
     1261 taatatcggt ttgtacatca gctatcatca caattggtac gatcattccg actcgcgtca
     1321 cccacgtgta aggttttatt ctgcccacgt ttacaacaat tattatgatg gtattgccaa
     1381 atatggtgtc ggttctacta tgggttcgtc ggtatttgtc gaaaacaatt ttttccgaaa
     1441 ttgtaagtac ccgatgctaa cctccatgca gggcaccgat atttattatg actccggcgg
     1501 tacattttcc ggcgaagacg gcggtacaat caaggctttc aacaatagta tcagcgggca
     1561 aacacgttat gtgccttacg atgctgccaa ctttccggtg gaatttgatg ctatcgaaac
     1621 tgctacgcgg gaacagacta tcagcaacac catcaaatcg aaacagggtg caaatgttta
     1681 taataatttt gatactgatg cttcgctcta tgtcaaaacc ttcattccgg atgaacctga
     1741 agccgccaga aacaaggtaa tagtctatgc cggacgtgta accggcggtg atttgaaatg
     1801 gacgttcaac aattcggtgg atgatgcttc ttacgccgtt aatacaggtt tgaaagctgc
     1861 tctcacagca tacaaaacaa aactggtagc agttcagggc gaaaatacat ctcccgaaag
     1921 tactcagacg ctgacttcat ccggaaatca caatcagaca gttagcgtgg gttctgcaat
     1981 aaccgatatc gtttttacct ggggaggtga tgcaactgat gttactgtta cgggcttacc
     2041 ggcatcgggt attacctatg tgaaaaatcc ggccaccaaa acaattacag tttcaggcaa
     2101 tccttcggca acggtaactt tcgctgttac tacaacgggt acatcagtta ctccggtaag
     2161 tattaccgga acgatagaag taactcagtc aacaggtggt acgtcattta ttcacaactt
     2221 tacaaccgat ggtaaaaaca gcactttcta tactataacc ggtaaccttt ctaccagtta
     2281 tggaacggta agttacaatg aattaacact gacgcagtgt ctgaaaatgg aaactagtac
     2341 caatatttca ttcacaacgc aacaaaaatc aaccttaacc ctggtttaca ataaaaccta
     2401 tacaggaaca ataaaaatag acggtgtaac ttatacacct tcaaatacag atggaattgt
     2461 aacaataacg tcactaccga ttggttcaca taccattctg aaaggctccg gttccggtta
     2521 tctcttctac atgagtattg tttatgaaaa ttcaacagat atagtaaatt ctgaattaac
     2581 agatataaaa atatatccca atccggttac cgataaactg aatatcattt ctcccaacag
     2641 actacatttc gtagaaattt atacctttag cggacaattg atatttaaag tcaacgggaa
     2701 tatgtctcaa gtggatatta gtaaactatc taaaggaaat tatattgtga aacttgtttc
     2761 tgaagctggt gtggtttcaa aactaatcat gaaagagtaa tcatattatt atgaaaatgt
     2821 catatttaat tgtcttatgc agctttttaa tacttgctac accagccagg acgcaaatat
     2881 ctgacacctg gatttctgat cagggtaatg gtacttatag aaatccggtt ctgaatgctg
     2941 actattccga tccggatgtg tgcagggtag gcgatgacta ctttatgact gcatcgagct
     3001 tcaattgtgt acccggtttg ccaattcttc attcgggtga tctggtcaac tggcagctca
     3061 ttggacatgc cctgaaccgt ctgatgcctg aagaagttta cacacattcg cagcacggca
     3121 atggagcgtg ggcgccatcc atccgttttc acaacgatga attctacata tattatcccg
     3181 accctgattt tggcatttat atgctgaaag caaaaaatgc cacaggtccc tggtcagacc
     3241 cggtgttggt caaagccggt aaaggattga ttgatccttg tccgctctgg gatgacgacg
     3301 gacgtgttta tcttgttcat gcctatgcag gtagtcgggc tggaataaaa agcatcctgg
     3361 ctgtggctga aatgaatgcc gatggaaaca aaattattag tccaagtcgt acagtctatg
     3421 atggtcacga agtcgatcca accattgaag gaccaaaatt ctataaacga aacggctact
     3481 attatatttt tgctccggct ggtggtgttg caaccggctg gcaggtggtg ctgagatctc
     3541 aaaacgtgta cggaccttat gaacgcaaag tggttatggc tcagggaaaa accggtgtca
     3601 acgggccaca tcagggtgca tgggtggaaa cacggcaagg cgaagactgg tttattcatt
     3661 ttcaggacaa aggtgcctgt ggacgtgttg tacatttaca gccgatggct tggaaagaca
     3721 atggatttcc ggtaattggt gttgataaag acggtgatgg atgtggtgag cctgtactag
     3781 agtacaaaaa accaaaaatt acaggcaatt tcgcaattca aactcctcct gaaagcgatg
     3841 aatttaatgg tgagtttggt tttcagtggc agtggcatgc caatccgcaa gactggtgga
     3901 gcttttcgga tgtccggaaa gggatactga ccttattctc agtttctttg ccggataaca
     3961 gtaaaaattt atgggacgtc ccaaatttac tccttcagaa atttccttca gataaattta
     4021 caactactgt aaaatttaat tttattccgg acgaacggtt tgacggcgaa agggctggtc
     4081 ttgtcataat gggattggat tatgcattgc tttccatcga aaaaaagaag gaaggttttt
     4141 ctatttcaca aaatgagtgc ttgggtgcag aaaaaggtaa caccgaaata aaaaatgaaa
     4201 ctgtttctgc agaaaaatcc gaggttttct tccgtgtgaa tgtaactcag gatgccatgt
     4261 gtttcttttc gtacagcgca gatggaaata aatacaccaa catcggaaaa ccgtttaaag
     4321 ctcgtgaagg taaatggatt ggagcaaaag tgggcgtttt ctgttcgaga cccctgaaaa
     4381 ataacgatgg cggcaaaatg gaaattgact ggttcagaat aagtaattaa taatcgacct
     4441 ggtaatgtta cgcaaactta cattatatat actgttcaca ggcttttgtg ctcttactgt
     4501 agctcaaaac aaaacaaatt ctcctgttcc ccgggataca agttatacat tatatagtac
     4561 agtaataaaa atccgcaaga attatccgga tgcgaggttg gtctctccgg ctattccccg
     4621 cgatattatt acatataaaa atattattta caaaactatt cctcaaacac cttacggaaa
     4681 acggaagttg tatctaaaca tttacagacc ttcaaaaatc ggcagactac cggtagttct
     4741 gcttattcac ggaggcggat ggagttccgg ttcgcccctg ttgcagcagt caattgctgt
     4801 tagtctgtgt atgaaaggat ttgcagctgt tgctgttgaa tatcgtctgt cgcccgaagc
     4861 aatttatcca gctgcagtcg aagacttgaa cgatgccctt gcgtgggttt gtaataacgc
     4921 ccgaacatac cgtttcgaca aaaataaaat agccgttcaa ggatgttcgg ctggtggtca
     4981 acttgcagct ttgctcggca tgaggaataa agatcgtttg attcgggctg taattaatat
     5041 cgacggaatc tccactttta tggatcagac aaccattgac cgggctgaaa attttcgcaa
     5101 aaacggtgga aaaatgcctg cagatatgca gtggcttggt ggaactatgt ccgagaagcc
     5161 ggaagtgtgg aaggatgcgt ctgccgttta ttttgtaaac agacattcag cgccggtttg
     5221 ttttatcaac agttctctgt cgcgttttca caacggacgc gatgagttaa tccgtaagct
     5281 caaaaaatac aatatctatt ccgaagttca tacttttgag aattcaccgc attcgttttg
     5341 gttgtttgat tcatggcacc aacctactat tgattatgcc tctgattttt taatgaaagt
     5401 atttgctgtg cagaaaaatc actaagcatc cgggttcgta gcaaatacat tgattatatc
     5461 tgttttagtt tgaatggtaa tatctttccg ggatattttc tgaattatat attggaatga
     5521 gcaggggagg aagatcgtaa aaacgaaaaa ccgcattgtc acttgttgag aaaactccta
     5581 taaataggat ttgaggcatt ggttctttct gtaatctgct gttccgacgt atcggaatac
     5641 cttgcggcgc agcccgccat taaaaaccgt ttgttactca tataaaattt ctgttgagtt
     5701 ttccaatctc tgtacaatgc ggttcacaaa tataatactt ttttcagtac aaaggttcat
     5761 tttagcgtta atttttttgt taattgttga tactaatctg tgtataaatt gcttctgaac
     5821 gaaatataag ctaaaaaaac agacatatta tttcggtata tgaaagaaaa actttacttt
     5881 tgtggttcaa aaataatcaa taataaatca taaaatattt atgtcaaaaa aagtagtaga
     5941 atctgcagaa cttgaaaatg tggaacgcgc tttaagttca tccgaagcat tcattgaaaa
     6001 atatcaaaaa caaattttga ttggtgttgg tgttgtggta cttgtcgtgt tagctgtaat
     6061 ggctttcaat aacttttatc tgaaaccaag agaagttgcg gcagaaaatg ccatgtacaa
     6121 aactcaggaa tactttgccg tagattcttt taaagttgcg cttgatggcg acggtaaaga
     6181 tgtgatgggc ttcaaagaaa ttgcatcgga gtacggaatg actgcaagtg gcaacctggc
     6241 taatgcatat acgggaattt gttattataa actaggtaaa tatcaggaag ctgtaaaata
     6301 cctgacacaa tacgatgctg aagatgattt ctttaaaact actattatcg gtttgacagg
     6361 cgattgctat gccgagatgg gagaaacagg aaaagctcag gattattaca gcaaagctgt
     6421 tgctgagaag aatgaactta gtcccatcta cctgaaaaaa gccggtatcc tctacgaaac
     6481 aaaaggacaa actgccgagg cagaaaaaat gtatcttcag ataaaaaatg attatccgaa
     6541 aagcatggaa gcgggtgaca ttgacaaata ccttgcccgg gtacaaaaat aaaattttat
     6601 taagcaatat atgtaaagct gctttctggt tcggatagca gctttattgt taaatatcct
     6661 aataatcaat tacaaataca tcagattatg gctacgcaat accacaattt atcgacttac
     6721 gatccggatc ttatgcccga caaggcaaaa gttgccgaac agaaatacgc cattgccgtg
     6781 gctgaatgga acccagatat taccgaagct ttacttgatg gagctgtaaa tacttttctt
     6841 gagaacggag cattgataga aaacattaca atcgttcatg ttcccggtac ttttgagctt
     6901 agctttgcag ccaaacgatt ttatgaagaa tattctcagg ccgacggaga taataaaata
     6961 cacaaatact cagctatcat cgtactcggg tgtgtcgtgc agggtgaaac accacatttc
     7021 aattatgttt gtcaaggtgt aacagcgggt atcaccgaac tcaatctccg cccagatggt
     7081 tgtccggtta ttttcggtgt gctgactacc aatacattac aacaagcaaa agaccgtgcc
     7141 ggtggaatgc atggaaataa aggggacgaa gccgcaatta ctgccataaa aatggcaaat
     7201 attgtttggt aatttcaaaa atatagctat ctttgcactc gcttttgata agtcactggg
     7261 cagttaccag agtggccaaa tgggactgac tgtaactcag ttgtctttcg a
//
LOCUS       DAOPYB010000155         7284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12415_length_7284_cov_9.065984, whole genome shotgun sequence.
ACCESSION   DAOPYB010000155 DAOPYB010000000
VERSION     DAOPYB010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12415_length_7284_cov_9.065984"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2582
                     /locus_tag="PKH58_10455"
     CDS             <1..2582
                     /locus_tag="PKH58_10455"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNX89494.1"
                     /translation="DKKNLGGIIFSILKRSVLEHLTNVGLLPNYAFPETGVVLNAQIR
                     NRNLTGVGDNNYSAKSIELIRPAKSAIRELVPENYFYTQGYKLRISGLNIISWNEEVV
                     NYRFCCKCDLIEIDSGQVGACPKCGDLSWGSANNKHKFVQLKSVKSFNDEADATLDDT
                     SEDREEKISLISTHFKFAANSTRGAYAMVKIPFGIEYLQDVEIFQVNTGLIDYSNSRE
                     LTINEFRVPVMGYVTCKFCGKSTSSPFEENRHYLWCKKRDEVYQNKADDVFEEIYLFR
                     HLKTEAIKILLPVQEFNSESTIAMFKSGIELGLKHYYKGNPQHIEIKEYKEFNKQTSR
                     NDRFLVLYDVIPGGTGYLSKLFNKTDFATLIEKAYLAIKDCTCQFSGKDGCYRCIYTY
                     GNQFEREELSRFRAEELFRKLQQASDKWDYIPEGLNEITNSGKIEESELEERFIRCIK
                     NYCEKDEQQLLDWKFETVNQDGIITYRLKIVNGDYAYHYQITPQVSLGQSDGVLYPTR
                     CDHQITCVDIKRDEESLPMEIIDSIPKIAVYLDGYQYHATAENNRFVGDIIKRIAIKQ
                     SNRYITWTLTWDDLTFFENSMAGQPNDIDEFYKNVRDCNQNYIKLQRAPLYKGFDEML
                     KTKRNNLERLFYILSNFDSEDLLSGKTTRQLLFGLQAAFLSAPMTEETAVKLLDEPEV
                     DLSKIEKHKSEDCYLYIDKTPISEEIDSRIFMNLNQHKMISRVNIPNNNEGYEKKGWE
                     NFWLLFNWAQFVLAEYSICIDGSQPAQDKEGEASTGDIFDNFEPELHPIIKQLLDKDI
                     EFNRTTSFFLEDKYGSIIAEAALGINRKYFINAFDEESRVKFIAAGYKELFIDSFNIN
                     EII"
     gene            2585..4663
                     /locus_tag="PKH58_10460"
     CDS             2585..4663
                     /locus_tag="PKH58_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX89495.1"
                     /translation="MRLYLYEKFMDAFILLPKGIQKKTMEFMDKFKQNPKSMAIHLEK
                     IKDFRDENLRSARIDQAYRAIIHVSKSDEVYHLLWAAKHDDCYTWAKNKVFEWNSNTQ
                     SYQIFDVAEEIINKADVEAMGPFREYTDQQFEQIGVPAVLLPSVRAVADSENLKLIEK
                     YLPQEVYERLFFLIEGISMDEIVADVEDGKVVIEDGQTDTVNNKRYFFEITDKNLETI
                     FSSDFQKWRIFLHPSQLKVVNSSNKGSVKVTGLAGTGKTVCALHRAKFITDNFVTSNN
                     KPLLFTTFTRSLVSTLKESLTELGVSETLVEVKHIHGLAIDLAKKHKLITEKVKILDF
                     FDHDYKIKIWEDILDSTISHFDADFFSSEYTDIVVQNNIITEEEYLKVSRVGRSQKLG
                     RKDRIEIWKIFETYNQKKKAENYVELGEALNLLNEYFIKEKNKPYSFIIADEIQDFSN
                     VELRLIRSMTDEHDNDLFLVGDPYQKIYARKINFSRSGINVRGNRSKQLKINYRTTDE
                     IRQLAITTVQGIDYDNFDGDNESSKGYISLTHGEIPAYSMFDKESEEFEFISSRIEDF
                     VSKAPGISFNNVCIACRTKSALSDIKKFIHNAKIPYYDVTTSTGNATGVHLSTFHNLK
                     GLEYKIVFLSGVNSKSVPLRPGNYDQWDRAQQFGFDILEKSLLYVAMTRAIQQLYITG
                     TGKRSEWVRV"
     gene            4702..5244
                     /locus_tag="PKH58_10465"
     CDS             4702..5244
                     /locus_tag="PKH58_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89496.1"
                     /translation="MKNNLDMIPRVLNSKNIDKAIHDYNELLKGIPLELKSNDVLHFL
                     QELKRGKLKNGPFPGISIFEAANRIMTDLTILYGVRDLLKGVVEEIKFDSYTVEFENE
                     NRNDHDIMADNSEQKLIGEAFNVAKSFFYTKKSSVLKKLKRSVKRDTILLLLFNEDAL
                     PEKYNTEKLENEFHLAVKIF"
     gene            5337..6629
                     /locus_tag="PKH58_10470"
     CDS             5337..6629
                     /locus_tag="PKH58_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89497.1"
                     /translation="MENIKNWGLLGSEKLKSERRTRTLGLGAAIRSFNELAVPGIGGL
                     WYEKQLFLSALGIVVAETARREGKRVSNIEVANAIEALGCWLAFNNNGWKRDSRLRGK
                     VKMKMQSDLSFQNVVKRSFYVTQPMRMQMVQPLLKLGFVMASGERFNSFLLSEDGYTL
                     IETMSSNTTIDRLPIINFMARWVAGCNRALNSPELLKILSPITKVPKDCKNFLLERLI
                     GGNSVEAIRRRNAYNWIITLSETSVTSWEQKPEMIEGHHWLDLRTGAIFFQVRDSALV
                     LLDSIERILSQSANNQISVKSCIINEVINNYNVLKKQASEFIIKNYDPTPAKMASAFC
                     KEICDNDIIYCLKSLILRDDRVLQIDNDTIKAGPAFRMINNSQSDDVDDAEDENENIE
                     IKDLTIFPGKISYRIHNLYLLHLDYTNNLNNWLKNEEQ"
     gene            6616..7242
                     /locus_tag="PKH58_10475"
     CDS             6616..7242
                     /locus_tag="PKH58_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89498.1"
                     /translation="MKNNSNYARPSLAQLFESPVGFKGKFGWLCGYSGDRAFLNDAAD
                     RFTQLSKEQRAQSGRISLAIMLDKHNPQITTKDVPGVYHLPIIAEQDSQFRLLHAKVA
                     ILLFCSDDSSDDHIIRLIVSTGNWTRSTLEYSLDLAWYTEYSNKVLSKNNDKNSQART
                     DIKAAWNMIAWLRQMFDTRMLTYIPANRETTETSDLYNQFEQKITALF"
BASE COUNT         2467 a         1195 c         1551 g         2071 t
ORIGIN      
        1 aggacaagaa gaatctcgga ggaattatat tttcgatatt aaagcggtct gtgctcgaac
       61 acctcacaaa cgtaggatta ttacccaatt atgctttccc tgaaacagga gttgttttaa
      121 atgcccaaat cagaaacagg aatcttactg gggttggaga taataattac tctgcaaaaa
      181 gcattgaatt aatccgacct gctaaatcgg ccatccggga attggtgcct gaaaattatt
      241 tctacacaca gggatataaa ctcagaatct cggggcttaa tattataagt tggaacgagg
      301 aggttgtaaa ctaccggttc tgctgtaaat gcgacctgat tgaaattgac agcggccagg
      361 ttggggcgtg cccgaaatgc ggcgatttat catggggctc ggccaataac aagcataagt
      421 tcgttcagct aaaatccgtc aagtccttta acgacgaagc ggatgcgaca ttggatgaca
      481 caagcgagga cagggaagag aaaatttcgc tgatttcaac ccacttcaaa tttgcagcta
      541 attcaacccg tggagcgtat gccatggtga aaataccttt tggtattgag tacttacaag
      601 atgtcgaaat atttcaggtg aatacgggat taattgatta ttcgaattcc cgggaattaa
      661 ctataaatga attccgggtt ccggtaatgg gatatgtaac ttgtaaattt tgcggaaaat
      721 caacaagtag cccttttgaa gaaaaccgac attatttgtg gtgtaaaaaa cgtgacgagg
      781 tctatcaaaa caaggctgat gatgtttttg aagaaatata tctgttcagg cacctgaaga
      841 ctgaagcaat aaaaatatta ttacctgttc aggagttcaa ttcagaaagc actatcgcta
      901 tgtttaaatc aggtattgaa ctcggtctaa agcactacta taaaggcaat cctcagcata
      961 ttgaaataaa agaatataaa gaattcaata agcaaacctc ccgtaacgac agattcctgg
     1021 tgctctatga tgtgattccg ggcgggacag gctatctttc gaagttgttc aataaaacag
     1081 atttcgcaac acttattgag aaagcttatt tggcaataaa agattgtact tgtcaattct
     1141 ccggtaaaga cggttgttac cgctgtattt atacctacgg caatcagttt gaacgtgaag
     1201 aattaagccg gttcagggcc gaagagttgt tcagaaaatt acaacaggca tcagataaat
     1261 gggattatat tcccgaaggg cttaatgaaa taactaattc gggtaaaata gaagagtctg
     1321 aattggaaga gcggtttatc cggtgcatta agaattattg tgaaaaggac gaacagcagc
     1381 tgctcgattg gaaatttgaa accgtcaatc aggatggaat aataacgtac agattgaaga
     1441 tcgtgaacgg cgattatgca tatcattacc aaattacgcc gcaggtgagt ttagggcaaa
     1501 gtgatggggt gttatatccc acccggtgcg atcatcagat aacttgtgtg gatatcaaaa
     1561 gagatgagga atcattgcca atggaaataa tagacagcat ccctaaaatt gcggtatatc
     1621 ttgatggtta tcaatatcac gcaacagcag aaaacaacag gtttgtaggg gatatcatca
     1681 aacgtattgc cattaagcaa agcaatcgat acataacctg gacccttaca tgggatgacc
     1741 ttacgttttt cgaaaacagc atggctggac aaccgaacga tattgatgaa ttttacaaaa
     1801 acgtcaggga ttgtaaccag aattatatca aacttcaaag agccccgctg tataaaggtt
     1861 ttgatgaaat gctgaagaca aagagaaaca accttgaacg attattttat attttgtcca
     1921 atttcgattc ggaagattta ttatctggta aaaccaccag gcaattgtta tttgggttgc
     1981 aggcagcatt tttatcagca cccatgactg aagaaaccgc ggtgaaattg ttggatgaac
     2041 ctgaagttga tttgagtaaa attgaaaaac acaaaagtga agattgttac ctgtatatag
     2101 ataaaacccc gatctcagaa gaaattgaca gtcgtatttt catgaatctg aatcagcata
     2161 agatgatctc cagggtgaac attcccaata acaatgaagg gtacgaaaag aaaggatggg
     2221 aaaacttttg gttgttgttc aattgggcgc agtttgtact cgctgagtac agtatttgca
     2281 ttgatggctc acaacctgca caggacaagg aaggcgaagc ttcgaccggc gatattttcg
     2341 ataactttga acctgagctc catccaataa taaaacaact acttgataag gatattgaat
     2401 tcaacaggac tacatccttt ttcctggagg acaaatatgg aagtataatt gcagaggcgg
     2461 ctcttggcat aaataggaaa tactttatta atgcctttga tgaagaaagc cgggtaaagt
     2521 ttattgctgc cggttacaaa gagttgttta ttgatagttt taacattaac gaaattattt
     2581 gatcatgagg ctatatttat acgaaaaatt catggacgcc tttatcctcc tgccgaaggg
     2641 gatacaaaag aagacaatgg aattcatgga taagttcaag cagaatccaa aatccatggc
     2701 gattcatctt gaaaaaatta aggatttcag ggatgaaaac ttgcgttcag cgagaattga
     2761 tcaggcatac cgggccatta ttcatgtgtc taaatctgat gaggtatatc acttgctgtg
     2821 ggcagcaaag catgatgatt gttacacctg ggctaaaaac aaagtattcg aatggaatag
     2881 caacactcag tcctatcaga ttttcgatgt ggctgaagaa atcatcaata aagctgatgt
     2941 tgaagcaatg gggccattcc gggaatatac cgatcagcaa tttgagcaaa ttggcgtccc
     3001 tgcggtatta ttaccaagtg ttcgggctgt tgcagattct gaaaatttga agttgattga
     3061 aaagtatctc ccgcaggaag tttatgaacg gctgtttttc cttatcgaag gcatttcaat
     3121 ggatgaaatt gttgctgatg ttgaagatgg taaggtagta attgaagatg gccaaacaga
     3181 tacagtaaac aataagcgtt atttcttcga aatcacagac aagaacctcg aaacgatatt
     3241 ttcgtcagac tttcagaaat ggaggatatt ccttcaccca agccaattga aagttgtgaa
     3301 ctcgtcaaat aaaggcagcg tgaaggttac tggtttggca ggaacaggaa agactgtatg
     3361 tgccttgcac agggcaaaat tcataaccga taattttgtc acctcaaaca acaagcctct
     3421 gttattcaca acatttacca ggagccttgt ctcaacgcta aaggaatcgc taacggagct
     3481 aggcgtaagc gaaactttag tcgaggttaa gcacattcat ggattggcca ttgatttggc
     3541 gaaaaaacac aagttaataa cggaaaaggt caaaatactt gatttttttg atcatgacta
     3601 caagattaaa atatgggaag atatactgga ttcgaccatt agccattttg atgcagactt
     3661 cttcagtagc gaatacaccg atattgtggt acaaaacaat attatcacag aggaagaata
     3721 cctgaaggta tcaagggttg gacgttcaca aaaattgggt cgtaaagacc gtattgaaat
     3781 ctggaagata ttcgaaacat acaatcagaa aaagaaggcg gaaaattacg ttgaattggg
     3841 ggaggcgttg aacttgctga atgaatattt tatcaaagaa aagaataagc catattcatt
     3901 tattattgct gatgaaattc aggatttctc caatgtcgaa cttcgtctta ttcgctcgat
     3961 gaccgatgag cacgacaacg atttgtttct tgttggagac ccttatcaga agatatatgc
     4021 ccggaaaatt aatttttcaa gatcgggcat aaatgtccgt ggtaacagaa gcaaacaact
     4081 aaagataaat taccggacaa ctgatgaaat aaggcagttg gctattacaa ccgttcaggg
     4141 tattgattac gacaatttcg atggagataa tgaaagttca aaagggtaca tctctttaac
     4201 ccatggtgaa atacctgcat attcaatgtt tgataaggaa tcagaggaat ttgaatttat
     4261 cagttccagg attgaagatt ttgtttcaaa agctccgggt atttcgttta ataatgtttg
     4321 tattgcttgc cgcactaaat cagctctgtc agacattaag aaattcattc ataacgcaaa
     4381 gatcccatat tatgatgtga ccacttcgac tggaaacgca acaggggtgc atctttcgac
     4441 ttttcataac ctgaaagggt tggaatataa aattgtattt ttatcgggtg taaactcaaa
     4501 gtctgtcccg ctgcgtcccg gcaattatga tcaatgggac cgcgcccaac agtttggatt
     4561 tgacattttg gaaaaatccc tgttgtatgt ggccatgaca agggcaatac agcaattgta
     4621 tatcacggga accggaaaaa gaagtgagtg ggtaagggtg taaatttgaa caatcaaaaa
     4681 attaacctaa aatactcgct tatgaaaaat aatctggaca tgatccccag agttttgaat
     4741 tcaaaaaaca ttgacaaagc aattcatgat tacaatgagt tgttaaaagg aattccattg
     4801 gaactaaaat ctaatgatgt gctgcacttt cttcaggaat tgaaaagagg aaaattaaaa
     4861 aatggtccat ttccgggaat atcaattttt gaagctgcaa accgtattat gactgattta
     4921 acaatattgt acggtgttag agatttacta aaaggtgtgg ttgaagaaat aaagtttgat
     4981 tcctacactg tggaatttga aaatgaaaac aggaacgatc acgatattat ggccgataat
     5041 tccgaacaaa agttgattgg agaagcattt aatgtggcaa aatcgttctt ttacacaaag
     5101 aaatcctctg tattaaagaa actaaagaga tctgttaaaa gagataccat attactatta
     5161 ttgttcaatg aagacgcgct tcctgaaaag tataacactg aaaagctcga gaatgaattt
     5221 catctggctg tcaagatttt ttaggaactg acaatattat atcgcaaaat gtttaattaa
     5281 gaatgagtaa tcatttattc aaagaatttg gcactttagg atagagaaaa tgtagtatgg
     5341 aaaatatcaa aaattggggt cttttaggtt cggaaaaact taaaagtgaa cgccggacaa
     5401 gaacacttgg tttgggtgct gctatacgtt cctttaatga acttgctgtt ccggggattg
     5461 gtgggctgtg gtatgagaag cagcttttct tatctgcttt aggaattgta gtggcggaaa
     5521 ctgcgaggag ggaaggtaaa agagtatcaa atattgaagt agctaatgcg attgaagctt
     5581 tgggttgctg gctggcattt aacaataatg gatggaaaag agacagtcga ttgaggggta
     5641 aagtgaaaat gaaaatgcag agtgatttat cttttcaaaa tgtcgttaag cgcagttttt
     5701 atgttaccca gcccatgcgg atgcagatgg tgcagccatt gttgaaattg ggatttgtaa
     5761 tggcaagcgg agaaagattt aattcttttt tattgtcgga agatggatat actctaattg
     5821 aaactatgtc atcaaatact acgattgaca ggcttcctat tattaatttc atggcaaggt
     5881 gggtggccgg ttgtaatcgt gcgcttaatt cccccgagtt attaaaaatc ctttcaccaa
     5941 taacaaaagt accaaaagat tgtaaaaact tcctgttgga aagactaata ggaggaaatt
     6001 cggtggaagc aattcgtcgc agaaatgctt ataattggat aataacctta tcggaaacgt
     6061 ctgttacttc atgggagcaa aaaccggaaa tgatagaagg ccatcattgg cttgatttaa
     6121 ggaccggagc aatttttttt caggttcgtg attctgcctt agtcttgctg gatagtattg
     6181 aaaggatatt atctcaatct gcaaataatc agatttctgt gaaatcatgt ataataaatg
     6241 aagtaattaa taactataac gtattaaaga aacaagcatc agaatttatt atcaaaaatt
     6301 atgaccctac tcctgcaaaa atggcttccg cattttgtaa agaaatatgt gataatgaca
     6361 taatatattg cttgaaatca ttaatattaa gggatgacag ggtcttgcag atagacaatg
     6421 acactatcaa ggctggccct gcattcagaa tgattaataa ttcacaatct gatgatgtcg
     6481 atgatgccga agatgaaaat gagaatattg agataaaaga cttgacaatc tttcccggga
     6541 aaatctccta tcgaattcat aatttatatt tacttcatct ggattataca aataatttaa
     6601 ataactggtt aaagaatgaa gaacaatagt aattatgcca gaccatcatt agctcagctt
     6661 tttgaatctc cggtgggatt taaagggaag tttggctggt tatgcggata ttcaggtgat
     6721 agggcttttc ttaatgatgc cgccgatcgg tttactcaat tatcaaaaga acagcgtgca
     6781 caatcaggaa gaatatcttt agcaataatg cttgacaagc ataaccccca gataacaaca
     6841 aaagatgttc ccggcgtata tcatttacca atcattgcag aacaagacag tcagttccgg
     6901 ttgcttcatg ctaaagtagc cattttgttg ttttgtagtg atgattcatc ggatgatcat
     6961 attattaggc tcatagtgtc tactggtaac tggacccgat caacactaga gtacagcctt
     7021 gatttagctt ggtatactga gtattcaaat aaagtacttt caaaaaataa tgataaaaat
     7081 tctcaggcaa gaacggatat aaaagcagct tggaatatga tagcatggct taggcaaatg
     7141 tttgatacta ggatgttgac atatattcct gccaatcggg aaacaacaga aactagtgat
     7201 ttgtacaatc agtttgaaca aaaaattacg gctctattct aaaatgtagt tgataaattc
     7261 attccttaat tactattgct gaca
//
LOCUS       DAOPYB010000156         7249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12496_length_7249_cov_7.995413, whole genome shotgun sequence.
ACCESSION   DAOPYB010000156 DAOPYB010000000
VERSION     DAOPYB010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12496_length_7249_cov_7.995413"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1144)
                     /locus_tag="PKH58_10480"
     CDS             complement(170..1144)
                     /locus_tag="PKH58_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89499.1"
                     /translation="MADLLNISASSTSGQGLTGIAPQQFDLKRYSSILLIPKDQGFTA
                     AERASITAFLRALQTRTTVDDRTVRMFPIHGITGIANSNTAPTEYTSGFGVKETIADG
                     SYFTTLTFRDGGLLRHANLNEFNGEDYDIMLVDSYGKFLGRVDSAGLFCGLTGKFTVI
                     PYDLPDDSNPSLYKATISFTDTKQLNQLSQIGFMDCDNQFNFATSVPGIVGLVLDARA
                     ATAAATINVGIKTVDGGVDMYDTYDDEFAYATLFRVVKTSDWTTEVTINSVTKNAGKY
                     WILALAAYTGEVAVYLEGPTTLAGKNIGGAPAMSYEAVDPLIVTLPAP"
     gene            complement(1176..1712)
                     /locus_tag="PKH58_10485"
     CDS             complement(1176..1712)
                     /locus_tag="PKH58_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89500.1"
                     /translation="MTDKLNISVFEKSEPDIYTILKNIVSTVSTLYGKSVFWTFGPGR
                     EIVESMIAMSNSSTSSAKKYPFIALVGNTPIQMGDSTCYGVATCQVIIGTLSDINTKA
                     EQRMVRSYQAVLQPIYKIFKQALFDSGKFDIQDIDKLEFEQVLNFNYAKGFDVEGMKY
                     PDIIDVIELRNLKLRIKY"
     gene            complement(1709..2230)
                     /locus_tag="PKH58_10490"
     CDS             complement(1709..2230)
                     /locus_tag="PKH58_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89501.1"
                     /translation="MLIDYTYFGGTGLFCVANVDPAIITNEGNYTKLVAFINAFEPEI
                     CEMLMTKALYDALIVGLAVTPTPEQRWIDLAAQLRNATAKTSPIANYVYFKLWSQSFQ
                     QSTQGGDVVITGSNLEPSANKEKAALIWNQAVRQFEDFQEWLSDHLDDYPEWDGEDVY
                     YLNCDDKINVLGI"
     gene            complement(2245..2886)
                     /locus_tag="PKH58_10495"
     CDS             complement(2245..2886)
                     /locus_tag="PKH58_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89502.1"
                     /translation="MKKIILICLMSLITFSVFSQSTTKTLPPLGRDSYYLKYTGSAWD
                     TLTSNRDTLRYVIEVNKEYPVLFYFNSVLNKISGIDTTVIVNVYGKVFSGQSWTLITA
                     ANALSSVVNTNTNVVSSLLTEPSYTGTITTTGSMTDTISADTTKKTLPYGELYYWTPT
                     YKVVQNSVTTSGRVALSNYYRYIMLEFIRTGDDHVGVGSKVTSFELRIFKRSF"
     gene            complement(2888..3061)
                     /locus_tag="PKH58_10500"
     CDS             complement(2888..3061)
                     /locus_tag="PKH58_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89503.1"
                     /translation="MQKVKVKLTGRHGHNIEGDIIECHPNLLPILEAQGKIASQEKEP
                     EKEKEVKTKKTKK"
     gene            complement(3065..4360)
                     /locus_tag="PKH58_10505"
     CDS             complement(3065..4360)
                     /locus_tag="PKH58_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89504.1"
                     /translation="MKKNEILDKIEDKNQQKNVAEILDFAEKAAENAKTTPETIQKEV
                     KEMLEKHEIKAESVKGLTDFIEEKLLSISETVKKLQENQVVDTKTIKGIIVSAFKNEA
                     TVSALKNIKKTNENVEVLTIKAVGEITTGNVTTTTGGNAILDLINTNDVNMQRLASTF
                     IENYATTSNTSVAVVPYADYLPKEGDVNFVLEGGTAGQVDLKIETRYATPKKAYGYEI
                     LTDDAIQDIPRLMSFAQQILLMKSMLKRQNGIIFGDGIGENPEGTTKIASAFDPASWT
                     GNKIKMPNLLDMIRAAANQIYVTQNFDDEIPFMPNVAFVNPADWFAFIGTKTEEGVYV
                     FPQFTFGENNKVDTFVVIPKINIPAGYIHVGDFSKLNIVNYINYQVVVGKINDQLIKG
                     RTTIAGFTRFFSYVKEYDKKGFIYDTLANIEAGIKEAEL"
     gene            complement(4381..5202)
                     /locus_tag="PKH58_10510"
     CDS             complement(4381..5202)
                     /locus_tag="PKH58_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89505.1"
                     /translation="MKIQIPYFEKPEQKYAYLRQHKTTMLAMKKASIKHADGVCTSFY
                     LPEKSIKQASKELVLPEKPETLNKIHIVPVINTTNILDSHNDVHINGIWNRSVNDTKR
                     KRLLLQEHLMKFDHIISDEVSVSVKTVDWAYLGESYPGKTEALIYDCLAERQRNDYMF
                     NNYVKGWVDAHSVGMLYIQLFLCMNSDSKYDVEEKNNWEKYYQLIVNKEMADNNGYFW
                     AVTEAKEIEGSAVPIGSCPVTPTMIVEDAEGSEKSTLINAPEKSTHKNAYSLFNY"
     gene            complement(5273..6535)
                     /locus_tag="PKH58_10515"
     CDS             complement(5273..6535)
                     /locus_tag="PKH58_10515"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNX89506.1"
                     /translation="MKIGKLHVGLVKKTSTPLVNNGLNDLMLKLPYYFPTDWISGINT
                     GSKIGLDTTTIEGQRNAYVFCPAITAIINYKVSATCNGRFVFKNSEGKEIGSNFQKLT
                     SRPNPDQNWIQFEQQFKTFQQIFGKAYIYVKNLPSIDIPKEMYVIPNWELQINKGVSD
                     FFGNPIINYNWTKCSTGKTITIMPVDMMVINDTGTDIKNPYGNYSEGCSRLLSIGDAV
                     RNIIASYEARNQFLTNGGPPYLIAPERDITGTALLLPKDKQEVEEKFLNRYGYIRGKK
                     NIAIVTQPVKAIKIGTNPNDIGAFQEVKENWEECCRAYGAGLDYIFGLDRTTFNNLSE
                     AKKTLYQDTIIPETASDLLQIGYYFGMKEIFSADFSHVECLQKSEKDKVDLFNSLIKP
                     LSDAITAELLTIEEAKSILKGNANLLEL"
     gene            complement(6719..>7249)
                     /locus_tag="PKH58_10520"
     CDS             complement(6719..>7249)
                     /locus_tag="PKH58_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89507.1"
                     /translation="RQGLVHPNVTWISEFPPDNELDKIGYGLDFGFTNSPTAIVKVGR
                     KGNNLYVKLLYYQPIADSNKLIEVVSTLIPKDAHITCDSSDKASNEAVGFIVLMRRAG
                     INAIPCRKFPGSIKFGIDNINKHCLHYVRDVDLRKEQENRVWRYVGGIPLNEPEPGFD
                     HAHDAIAYCLQTDFQN"
BASE COUNT         2079 a         1453 c         1231 g         2486 t
ORIGIN      
        1 tcccaaattt cagacaaata tccgtcaaga tctttcgtga aagaaatatg cccggttgat
       61 ttttcatact caataaaagc ttctttgctt tcaaacgacc tgacaaaagt tatattgaaa
      121 gagccgccgc ctaatttgac ggcgggctca atttttcttt gctttttcat cacggagctg
      181 gtaatgttac aatcaaagga tcaacagctt cataactcat ggccggggct cctcctatat
      241 tctttccggc caaagttgta ggcccttcga gatatacagc cacttcacca gtataagcag
      301 ccaaagcaag tatccaatac ttcccggcgt tctttgtgac gctattaatg gttacttcgg
      361 tagtccagtc agaagtttta acaacccgga aaagtgttgc ataggcaaac tcatcatcat
      421 atgtgtcgta catatcaact ccaccgtcaa cagttttaat tccgacgttg atagttgccg
      481 ctgctgttgc tgctctggca tccaaaacaa gtccgacaat gccaggaaca gaagtagcaa
      541 aattgaactg gttgtcgcag tccatgaagc caatttgtga aagctgattt aattgcttcg
      601 tgtcggtgaa tgaaatcgtt gccttataaa gtgaaggatt tgaatcgtcc ggcaaatcgt
      661 aaggaataac ggtaaatttt cctgttaaac cgcagaataa tccagcagag tcaacccggc
      721 ctaagaattt tccatatgaa tcaacaagca ttatatcata atcctcaccg ttgaactcat
      781 ttaggtttgc atgacggagc aatccgccat cacggaaagt caatgtagtg aagtaacttc
      841 cgtcggcaat agtttctttc actccaaaac cggaagtata ttcagttggt gctgtgttcg
      901 agtttgcaat cccggtaatt ccgtgaatag gaaacatacg gacagtacgg tcatctaccg
      961 ttgtacgtgt ttgtaatgct ctcagaaatg ccgttatgct ggcacgttca gccgctgtaa
     1021 atccctggtc tttcggtatg agtaaaatac tcgaataccg tttcaggtca aattgttgcg
     1081 gggcaattcc ggtaagtccc tgacctgatg tcgaacttgc ggaaatattt aataaatctg
     1141 ccataatttt tattctcctt tttcgttaat tatttttaat acttaattct taattttaaa
     1201 tttcttaatt caataacatc aataatgtct ggatacttca taccttctac gtcaaaacct
     1261 ttggcataat tgaaattcaa aacttgctca aattccaact tatcaatgtc ctgaatgtcg
     1321 aatttacctg aatcaaaaag agcctgttta aagattttgt aaatcggctg caaaactgcc
     1381 tgatatgatc ttaccatgcg ctgctcggct ttggtattta tgtccgacaa agtacctatt
     1441 attacctggc aggtagccac gccgtagcag gtgctatcac ccatctgtat cggcgtgtta
     1501 cctaccaagg ctataaaagg atatttttta gctgaagatg tcgagctatt cgacatagca
     1561 atcatgcttt ctacaatttc ccttcccggg ccgaacgtcc aaaaaacaga ttttccgtat
     1621 aaagttgaaa cggtagaaac gatatttttc aaaatcgtat aaatgtcagg ctccgacttt
     1681 tcaaaaactg atatgtttaa cttatctgtc atatgcctaa aacatttatt ttgtcgtcgc
     1741 aatttaaata atacacatct tcgccgtccc attccggata atcgtctaaa tggtcgctca
     1801 accattcctg aaaatcttca aactgtctta ctgcctggtt ccaaatcaaa gctgcctttt
     1861 ctttattcgc cgaaggctcc aaatttgacc cggtaattac aacgtctcca ccctgtgttg
     1921 actgctgaaa actttgcgac cataatttaa agtacacata gttcgcaatt ggacttgtct
     1981 tagcggttgc gtttcgcaac tgtgccgcta agtcaatcca gcgttgttca ggtgtcggag
     2041 tgacggccaa acctacaatc aaagcatcat aaagagcttt tgtcatcaac atttcacata
     2101 tttcaggctc aaatgcattg atgaaggcaa ccaattttgt ataattacct tcattagtta
     2161 ttattgccgg gtcaacattt gcaacgcaaa ataacccagt tcctccgaaa tatgtatagt
     2221 cgatcaacat tgatgaaatt gaaattaaaa tgaacgtttg aaaattcgta actcaaaaga
     2281 tgttacttta cttccaacac cgacatgatc atcgcctgtt cgtatgaatt caagcataat
     2341 gtacctgtaa tagttagaca acgcaacacg cccgcttgta gtaacagaat tttgaaccac
     2401 cttataagta ggtgtccagt aatacaactc tccataaggc aatgttttct tagtcgtatc
     2461 tgccgatatt gtatcagtca tgctcccggt ggtagttata gttccggtat acgatggttc
     2521 cgtcaaaagt gaagatacaa cattcgtatt ggtattaaca actgaactca aagcatttgc
     2581 cgccgttatc aatgtccatg attgaccgga aaatacctta ccatatacat taactattac
     2641 cgtggtatca ataccgctta ttttattcaa tacggagttg aaatagaata ataccggata
     2701 ctctttgtta acttcaataa cataacgcaa tgtatcgcga tttgaagtaa gtgtatccca
     2761 tgcgctgcca gtgtatttaa ggtaataaga atcacggcca agtggcggaa gagttttcgt
     2821 ggttgattgg gaaaatacgc tgaaagtaat cagcgacatt aaacagatta aaattatttt
     2881 tttcatttta ctttttggtt tttttggttt ttacttcttt ttctttttcc ggctcttttt
     2941 cctgtgaagc aatctttccc tgtgcctcaa ggataggcag taagttgggg tgacattcga
     3001 taatgtcacc ctcaatgtta tggccgtgtc tgcctgtaag ttttactttt accttttgca
     3061 tggattaaag ttcagcttcc tttataccgg cttcaatatt tgcaagggta tcgtaaataa
     3121 atcctttttt gtcgtattct ttaacgtatg agaaaaatct cgtgaatccg gcaatggtgg
     3181 tgcgtccctt aatcaactgg tcattaattt tgccaacaac tacctggtag ttgatatagt
     3241 ttacaatatt caatttggaa aaatcaccaa catgaatgta tcctgcaggg atatttattt
     3301 ttgggattac tacaaaagtg tcaaccttgt tattttcgcc gaatgtgaat tgcggaaaaa
     3361 cgtaaacacc ttcttctgtt ttagtaccta tgaaagcaaa ccagtcagca ggattaacga
     3421 aagctacatt aggcataaaa ggaatttcat catcaaagtt ttgtgttaca taaatctgat
     3481 tggcagcagc tcttatcata tcgagcaggt tcggcatttt aattttattg ccagtccaac
     3541 ttgccggatc aaaagcggaa gctatttttg tagttccttc gggattttcc ccaattccat
     3601 caccgaatat aatcccattt tgacgtttga gcatactttt catcaaaagg atttgctgtg
     3661 caaaactcat caggcgaggg atgtcctgaa ttgcatcatc tgttagaatc tcatatccgt
     3721 aagccttttt cggtgttgcg tatcttgttt caattttcaa atcaacctgt cctgccgttc
     3781 caccttcaag aacaaaattc acatctccct ctttgggtaa gtagtcagcg taaggaacaa
     3841 cggctacgga tgtattgctt gtagtagcat aattttcaat aaatgtagaa gctaaccgct
     3901 gcatgttaac atcattcgtg ttaataaggt caaggatcgc atttccgcct gtcgtggttg
     3961 tgacgtttcc ggttgttatc tctcccacag ccttaatagt aagaacttca acattttcat
     4021 ttgttttttt gatgtttttt aaagctgata ccgtagcttc atttttgaag gcagaaacaa
     4081 taattccctt aattgttttg gtatcaacca cctgattttc ctgcaatttt tttacagttt
     4141 cagaaattga caataatttt tcttcaataa aatccgtaag accctttaca gattcagctt
     4201 ttatttcatg tttttcaagc atttctttca cctctttctg tatggtttcg ggtgtagttt
     4261 tcgcattttc tgctgctttt tctgcaaaat ctaagatttc agctacattt ttctgctgat
     4321 ttttatcttc tattttgtca agaatttcat tttttttcat ttttaaattt ttattaatgg
     4381 ttagtaatta aataaactat atgcgttttt atgcgtggat ttttccgggg catttataag
     4441 ggtggatttc tccgagcctt ctgcatcttc gacaatcata gttggtgtta ccgggcaact
     4501 accgatagga acggctgatc cttcaatttc tttagcttct gttactgccc agaaatatcc
     4561 gttattatca gccatttctt tattaacaat caactggtaa tatttttccc agttattttt
     4621 ttcctcaaca tcatattttg aatcagaatt catacaaaga aacaattgaa tgtataacat
     4681 gccaacggaa tgtgcatcaa cccatccttt gacataattg ttgaacatat aatcgtttct
     4741 ttgtcgttcc gccaaacaat cataaattaa tgcctctgtt tttcctggat aactctcacc
     4801 aagatatgcc cagtcaactg ttttaacaga aactgaaacc tcatcagaaa ttatatgatc
     4861 gaatttcatc aaatgttcct gtaatagcag gcgttttctt tttgtgtcat ttactgaacg
     4921 gttccaaatt ccattaatat gaacgtcatt atgtgaatca aggatatttg tcgtatttat
     4981 gactggaaca atatgaattt tattcaacgt ttccggtttt tcaggcaaaa caagttcttt
     5041 agaggcctgt tttattgatt tttcgggcaa ataaaatgaa gtacatacac catcggcatg
     5101 ttttattgaa gctttcttca tggctaacat ggtagtttta tgctgtctta aataagcata
     5161 tttttgctcc ggtttttcaa aatatggaat ttgtatcttc atttttttac aatggttttt
     5221 tccttgatta ttctttcacg ttcctttata attttatcct tatccttatc gttcataatt
     5281 caagaagatt tgcgtttcct tttaaaatac tcttggcttc ctcaatagtc aataattctg
     5341 ccgttattgc atctgataat ggtttgataa gtgaattaaa taaatcaact ttatcttttt
     5401 cagatttttg caaacattcc acgtgagaga aatctgctga aaatatttct ttcatgccaa
     5461 aataataacc aatttgcaaa agatcgcttg cagtctcagg aattattgta tcctgataaa
     5521 gtgttttctt agcctctgaa agattgttaa atgtcgttcg gtcaagccca aaaatataat
     5581 caagtccggc gccatatgcc cggcaacact cctcccaatt ttcttttact tcttgaaatg
     5641 cacctatatc attgggatta gtgccaattt taattgcctt aactggttgt gtaacaattg
     5701 caatattctt ttttccccgt atatacccgt aacgatttaa gaatttttct tcaacttctt
     5761 gtttgtcttt aggtaaaaga agtgccgtac ctgtaatatc tctttccgga gcaattagat
     5821 atggtggtcc accatttgtt aaaaattgat tgcgagcttc ataactggca ataatatttc
     5881 taacagcatc cccgatagat aacaacctac tgcaaccctc tgaataatta ccgtaaggat
     5941 tttttatgtc ggttccggta tcgttaataa ccatcatgtc taccggcata attgttatcg
     6001 tttttccagt agaacatttt gtccaattgt aatttattat aggattgcca aagaaatcag
     6061 aaacaccttt gtttatctgt agctcccagt taggaataac atacatttct tttggtatat
     6121 cgatacttgg taaattcttg acataaatat atgccttacc aaatatttgc tgaaacgtct
     6181 taaattgctg ctcgaattgt atccagttct gatcaggatt aggacgggat gttaattttt
     6241 gaaaattgct acctatctct ttgccttctg aatttttgaa aacaaaacgt ccattacaag
     6301 tggccgagac cttataattt ataattgctg tgatggccgg acaaaataca tatgcattgc
     6361 gctgtccttc aatagtagtt gtgtcaaggc caatctttga acctgtatta ataccactta
     6421 tccaatccgt agggaaataa taaggtaatt tcagcattaa atcattcaaa ccattgttta
     6481 ccaatggagt tgatgttttt ttaactaatc caacgtgaag ttttcctatt ttcattcgtg
     6541 attatgaaac cacaaatata cggcataaaa ataaaaaagc aagtttttta ttaaaaaatg
     6601 ttaaaaatta aagtttggca cggagttaaa ataatatttt atttaatact tagttgttaa
     6661 attaaccaaa tattaaatac atagtcttca tttttcccgc tatttgagtt ttttacggtc
     6721 agttttgaaa gtctgtttgc agacaataag ctattgcatc atgtgcatgg tcaaagccag
     6781 gctcaggttc attcagcggg atgccgccca cataccgcca aactctgttc tcttgctcct
     6841 tacgtaaatc tacatcccgg acataatgca agcaatgttt atttatgttg tcaattccaa
     6901 attttatact tcccgggaac ttgcggcaag gaatagcatt tattccggcc cggcgcataa
     6961 gtacaataaa tcctactgct tcattacttg ccttgtctga actatcacag gtaatatgag
     7021 catctttagg aattaaagtg ctgacaacct caatcaattt gttactgtct gctattggtt
     7081 gatagtacag taatttcaca tataaattat ttcctttgcg tcctactttt acaatagccg
     7141 tcgggctgtt cgtaaatcca aagtcaaggc cgtaacctat tttatccagt tcgttgtctg
     7201 gtggaaactc acttatccac gttacatttg gatgtacaag tccctggcg
//
LOCUS       DAOPYB010000157         7217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12573_length_7217_cov_9.407149, whole genome shotgun sequence.
ACCESSION   DAOPYB010000157 DAOPYB010000000
VERSION     DAOPYB010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12573_length_7217_cov_9.407149"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /gene="sucC"
                     /locus_tag="PKH58_10525"
     CDS             <1..711
                     /gene="sucC"
                     /locus_tag="PKH58_10525"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011181.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNX89508.1"
                     /translation="EADLGMTPFIARKIAFQLFEDYNLVKQAADLFRKMYKICLDTDA
                     SLVEINPLVITDNNTLLALDGKMNFDDNALFRQPDIEKLFEPDENEMKEILAKEKGLS
                     YIKLDGNIGYMVNGAGLAMATMDMIKMYGGEPANFLDIGGSSNPQKVIDAMNLILSDE
                     NVKVVMINIFGGITRCDDVARGLLAALEQLTVNVPIVVRLSGTNAKEGLEILHGTNLQ
                     VVESMTEAAKKAIELSKI"
     gene            714..1589
                     /gene="sucD"
                     /locus_tag="PKH58_10530"
     CDS             714..1589
                     /gene="sucD"
                     /locus_tag="PKH58_10530"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNX89509.1"
                     /translation="MSILVNKNTRLIVQGITGRDGGFHTSKMKAYGTNIVGGVSPGKG
                     GQIVEGIPVFNTVDDAVKRTGANTSIIFVPASVAADSILEAAEAGIKLVIVITEGIPT
                     LDMVRLIPYLKKKGTLLIGPNCPGLITPDEALVGILPGNIFKKGNVGLISRSGTLTYE
                     MVNQLTTNGIGQSTCVGIGGDPVAGLYYQELLQMYQDDPETEAVVLIGEIGGDAEERA
                     AQYIKSNLTKPVVAFIAGQSAPPGKRMGHAGAIISSGTGTAAEKIAAFEAAGVPVARK
                     PEDIPVLVKRALQYG"
     assembly_gap    1865..1964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1965..2810
                     /locus_tag="PKH58_10535"
     CDS             <1965..2810
                     /locus_tag="PKH58_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89510.1"
                     /translation="NPKKDTASNPMIMFLVLNTAGLVLVPMTVMMYRAQFGAVNPADV
                     FLPIVVATLCAWLSGLILVSIIQKINLFNRVVMGVLISLVGFIVGLVYFFSRLSQEQI
                     TAYSSFAAAFIILSIIVVFVIMAVRKKINVYSTFIDGAKEGFQVAVGIIPYLVSILVA
                     IGMFRASGAMDLLVDGIRYLVAATGTNTDFVEALPTAFMKPLSGSGARGMMVETMKEF
                     GADSFVGRLSSIIQGSTDTTFYILAVYFGSVKITNTRYAVIAGLAADIIGILAAILLG
                     YLFFH"
     gene            2829..3254
                     /gene="ybeY"
                     /locus_tag="PKH58_10540"
     CDS             2829..3254
                     /gene="ybeY"
                     /locus_tag="PKH58_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNX89511.1"
                     /translation="MIQYIAEEVEMIEFDQSVINRWIKSIAAQYNRRPANISYIFCSD
                     ERILEVNKQYLQHDYYTDIITFDYSRGNTISGDIFISLDTVADNANQLGVEFRDELCR
                     ILIHGILHLCGQDDKTPEMRAEMTDKENKALERLRKDYH"
     gene            3460..4263
                     /locus_tag="PKH58_10545"
     CDS             3460..4263
                     /locus_tag="PKH58_10545"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNX89512.1"
                     /translation="MNVFFRRTILVLSFIVLLSFCSVWSQNEYRAEIGVQGGLNVYTG
                     DINTVAKPDYFLQNMQNMQPDFGMYFRYRFNPRTALRLGYDYSTENGAYRFSDFAGPR
                     TLDQQLHILDLTGEYNFFDLENNPYKSYSKKISPYIYAGLGIILLPDAESFFNKKYSP
                     SVPFGFGVKWKITERWNLNVQWSNHFCLGDNLEGIKDLEYGELIHISPNGRPIWNEGI
                     IPDWFNRINPMNNDLLSGLTLGISFDFWKKGCDCKKNGNNKKQRSGFQW"
     gene            4309..5031
                     /locus_tag="PKH58_10550"
     CDS             4309..5031
                     /locus_tag="PKH58_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNX89513.1"
                     /translation="MSFKEQLDLINLPQHVAIIMDGNGRWAKERGYDRIYGHQHGVSS
                     VRECTEAAAEIGLKYLTLYAFSTENWNRDRKEVEALMELLIDTIERETPTLDKNNISL
                     KAIGDLSRLPLGAYKKLMRCIEHTKTNTGLCLVLALSYSSRWEICNALRQISQEVKDG
                     KLSTDEISDELISSHLTTKEIPDPDLMIRTSGEIRISNFLLWQLAYTELYFTSTHWPD
                     FRKENFFEAIVEFQRRERRFGK"
     gene            5120..>7217
                     /locus_tag="PKH58_10555"
     CDS             5120..>7217
                     /locus_tag="PKH58_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNX89514.1"
                     /translation="MSAQTVVNPDTVVNPEIDYMEPKEYQIADIQVEGAESYDDFVLI
                     GFSGLQVGQKIVIPGDDITNAVKKFWEQSMFSDVKIELTKVAGDKAWLTIYLQLRPKV
                     SEIIYNGLKKAEIEDIEKRIGILKEKQITPNMSDRASKSIKKYLDEKGFSNADIKVYQ
                     KDDPAKPGFVKVEIDVDKKLKTRVHKIYVTGNENLNALEINKAMKKTNDNNIINIFRT
                     KKYVKDLYEKDKIAAIEKYNEFGYRDAYIVSDSVVRFDDKSVDIYLKFNEGNKYYFRD
                     IKWIGNTIYPYETLNQFLTIKKGDVYNLKLLNKRLQEDEDAVAKLYSNKGYLFFNIQP
                     VEVAIENDSIDFEIHMVEGKPATINEVKITGNTRVYENVVRRELRTVPGALYSQDDIM
                     RTLRELAQMGHFDPEKLSKDMMEGGLKPNPENGTVDLTYKLETKSSDQVEFSAGWGAA
                     GIVGSLGLKFSNFAIQNIFKPETYRIVPQGEGQTFSINARTNGNYYSSFSLSFLEPWL
                     GGKRPNSLSVSLYYTATSGMSDRYLNAYSSMYSSMYSGYGGYGYGGYGYGGYGSGYGY
                     GSGYGNTSYETEVDKNKYFRTLGASVGYGKRLSWPDDFFTFYGELSFQRYMINNWYQQ
                     ETNVTNGVFNNVGLNLTIGRNSIDNPIFTRKGSNFSLGLQITPPYSKFNGKDYSSSSM
                     TDAERYKFLEYHKWKFS"
BASE COUNT         2263 a         1301 c         1553 g         2000 t
ORIGIN      
        1 gaagccgacc tgggcatgac gccttttatt gccagaaaaa tagcgtttca gctttttgaa
       61 gattacaatc tggtaaaaca ggccgcagat ttattccgga aaatgtataa aatctgcctt
      121 gacaccgatg cttcattggt tgaaatcaat ccgctggtaa ttaccgacaa caacacactg
      181 ttggcattgg atggtaaaat gaatttcgac gataatgccc tgtttcgcca gcccgatata
      241 gaaaaacttt ttgaacctga cgaaaacgag atgaaagaaa tcctcgcgaa agaaaaagga
      301 ctgagctaca tcaaactcga cggaaacatc ggctacatgg tcaacggtgc cgggctggca
      361 atggcaacca tggatatgat caagatgtat ggcggagaac cggcaaactt tctggatatc
      421 ggtggaagtt ccaatccgca gaaagtgatt gacgccatga atcttattct ttcagacgaa
      481 aacgtgaaag tggtgatgat taatatcttc ggcggaatta cccgctgcga tgatgtggcg
      541 cgcggattac tcgctgcatt ggaacaactg acagtaaatg tgccaatcgt agtgcgttta
      601 tccggaacaa acgctaaaga aggtttggaa attctgcatg gaaccaatct gcaagtagta
      661 gaaagcatga ccgaagcggc aaaaaaagca atcgaactta gcaaaattta atcatgagca
      721 tattagtcaa taaaaacaca cgcctgatag ttcagggaat tacaggcaga gatggtggtt
      781 ttcacacctc aaaaatgaaa gcatacggca caaacatagt gggtggcgtg tcgcctggca
      841 aaggcggaca gatagtggaa ggaattcccg ttttcaatac agtggacgat gccgtaaaac
      901 gaaccggtgc aaatacttcc atcatttttg tcccggcatc ggtggctgcc gatagtattc
      961 ttgaagcagc ggaagcaggc attaaattag taattgtcat taccgaagga attccaacac
     1021 tggatatggt gcgtttaata ccttatctga agaaaaaagg cactctgctc ataggcccaa
     1081 actgtccggg attaattaca ccagacgaag cactggtggg aattctgccc ggaaatattt
     1141 ttaaaaaagg taatgttggg ttaatcagcc gaagtggtac gctcacatac gaaatggtaa
     1201 accagcttac caccaacggt atcggacaaa gcacctgtgt aggcattggt ggcgacccgg
     1261 ttgccggatt gtattatcag gaattattgc agatgtatca ggatgatccc gaaaccgagg
     1321 ctgtggtgtt gattggtgaa attgggggcg atgccgaaga aagagctgca caatacatta
     1381 aaagtaacct gacaaaacct gttgtggctt tcatcgccgg acaaagcgcc cctccgggta
     1441 aacggatggg acatgccgga gctattattt caagcggaac aggtacagcg gccgaaaaga
     1501 ttgcagcatt cgaagcagcc ggagtgcccg tggccaggaa gccggaagat attccggtgc
     1561 tggtaaaaag ggcgttacaa tacgggtaga agctccccgt gttctttcaa actgatacaa
     1621 atggtgtata aacaaaatat tttccagtat agaaaaaaag ccagtggatt tattatctat
     1681 tggctttctg tttttcggaa tgtgattttg tttgcctcat gcacttaaaa acaccaaaaa
     1741 aaagactatt tttgtgccaa actaacgaat atgttctatt tatggcacta aattatatct
     1801 ggattgcatt tgtactcatt gcctttgtgg tggcgttggt acaaatgatt ttttttggaa
     1861 atacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaatccg aaaaaagaca
     1981 cagcatccaa tccgatgatt atgtttctgg tactgaatac agccggtctt gtacttgtac
     2041 ctatgactgt aatgatgtat agggcacagt ttggagcagt taatcccgct gatgtttttc
     2101 tgccgatagt tgttgcaaca ctgtgtgcct ggctctcggg tctgatcctt gtttctatta
     2161 ttcagaaaat aaatctgttc aaccgtgtgg tgatgggtgt tttgatttcg cttgtgggtt
     2221 tcatagtagg attagtgtat tttttcagcc gactttcaca ggaacagatc actgcatatt
     2281 ctagttttgc ggcagctttt attatacttt ccattatcgt cgtgttcgtc attatggctg
     2341 tgcgcaagaa gataaatgtt tacagcacct ttattgacgg tgccaaagaa ggatttcagg
     2401 ttgctgtcgg tattattccc tatcttgttt ccatcttagt agctatcggt atgtttcgtg
     2461 catcgggtgc aatggatttg ctggtcgacg gaattcgtta tctggtagcc gctacaggaa
     2521 ccaataccga ttttgtggaa gctctgccaa ctgcttttat gaaacctctg agcggaagtg
     2581 gagcacgtgg tatgatggtg gaaacaatga aagaatttgg agcagattcg ttcgttggac
     2641 gactttcatc cattattcaa ggctcaacag acacaacttt ttacatcctg gcagtatatt
     2701 ttggctctgt gaaaattaca aatacacgat atgcggttat tgcaggtctt gctgctgata
     2761 tcattggaat attggctgct attttgcttg gatatctttt ctttcattaa ataaactgac
     2821 taagtactat gatccaatat attgccgaag aagttgaaat gattgaattc gatcagtctg
     2881 taataaaccg ctggataaaa tcaatagcag cccaatataa tcgcagacca gcgaatatat
     2941 catacatttt ctgttcggat gagcgtattc tcgaagtgaa taaacaatat cttcagcatg
     3001 attattatac ggatattatc accttcgatt acagcagggg aaacacaatt tcaggggata
     3061 tttttattag tttagatact gttgctgata atgcaaacca gctgggggtg gaattcaggg
     3121 atgaattgtg tcggatttta attcacggaa tactgcattt gtgcggacag gatgataaga
     3181 cacccgagat gagagcagag atgacagata aggaaaataa agccctcgaa agattgcgaa
     3241 aggactatca ctgaatgttt tttttacata aggtttaagt aaatcaaaag agttgtacat
     3301 tttgcaatta tttgttaaat taaccattct aattttagct gattacctta tattacaaag
     3361 attatcatta cttttgttac caaattcagt gacaaaaata aaatttatgg ttcaaaaata
     3421 cgttctacaa ttactgttta ctatttattt gttgagaata tgaacgtgtt tttcagaaga
     3481 accatattag tattgtcttt tatagtttta ttatcatttt gtagcgtctg gagtcagaat
     3541 gagtatcgtg ccgaaattgg tgtccaggga ggcttaaatg tctatacagg agatattaat
     3601 actgttgcaa agccggatta ttttttgcaa aatatgcaaa atatgcaacc agatttcggg
     3661 atgtattttc gctatcggtt taatccacgt acggcgttac gactgggata tgattacagt
     3721 acggagaatg gggcgtatag attttctgat tttgcaggac cccggacact ggatcagcaa
     3781 cttcacatac ttgatcttac aggtgaatat aatttttttg atttagaaaa taacccttat
     3841 aagagttaca gcaaaaaaat ttcaccctat atttacgcag gtcttggcat tattcttttg
     3901 ccggatgcgg aaagtttctt taataaaaag tattcacctt cagttccatt tggatttgga
     3961 gtaaaatgga aaataactga acgatggaac ctgaatgtac aatggtcaaa tcacttctgt
     4021 ctgggagaca accttgaggg aatcaaagat cttgaatatg gtgaactgat acacataagc
     4081 cctaatggca ggcctatatg gaacgaaggc ataattcccg attggttcaa tcgcattaat
     4141 ccgatgaaca acgacttact ttcgggatta acactgggaa taagttttga tttctggaaa
     4201 aaaggatgtg actgtaagaa aaatgggaac aacaagaaac aacgttcagg atttcaatgg
     4261 taattaataa gtgagaaagt taagtagttt tacaagttaa cagacgatat gtcattcaaa
     4321 gagcaactgg atctgataaa tttacctcaa cacgtagcca ttattatgga tggtaacgga
     4381 cgttgggcga aagagcgcgg atatgaccgt atttatgggc atcaacacgg ggtgtcctct
     4441 gtacgtgaat gtaccgaagc tgcagctgag attggcctaa aataccttac tctttatgca
     4501 ttttcgaccg aaaactggaa ccgtgaccgg aaagaagttg aagcattgat ggagttgctg
     4561 attgatacca ttgaacggga aacacctaca cttgataaaa ataatattag tttaaaggca
     4621 atcggtgatt tgtcgaggtt acctctggga gcatacaaaa aactcatgcg ttgtattgaa
     4681 catacaaaaa cgaacacagg tttatgtctg gtgcttgcat taagttactc ctcacgatgg
     4741 gaaatatgca atgctttacg ccaaatatca caggaagtta aagatggaaa actcagcacc
     4801 gatgaaataa gcgatgaatt aatttcaagc catcttacaa caaaagagat acctgatcct
     4861 gatttaatga tacgtaccag cggagaaatc agaattagca attttttact ctggcaactg
     4921 gcatacactg aactgtattt tacaagtaca cattggcctg atttcagaaa agaaaatttt
     4981 ttcgaagcga tagtcgaatt tcaaagacgc gaacgcagat tcggcaaata aacagaacta
     5041 ttcagaagat acctaacaga ctcacatgca atacaaaaca gtaattattt tttgttttct
     5101 gctccttttc agtcatctta tgtcagccca gactgttgtc aatcccgaca ctgttgttaa
     5161 tcctgaaata gattacatgg aaccaaaaga atatcagata gctgatattc aggttgaagg
     5221 cgctgaatcg tatgacgatt tcgtactcat tggattttcc ggccttcagg ttggacaaaa
     5281 aattgttatt cccggtgacg atataactaa tgcagtaaaa aaattctggg aacaatccat
     5341 gttttctgac gttaagatag aacttacaaa agttgccggt gataaagcct ggcttacaat
     5401 ctatctgcaa cttcgtccaa aagtatcgga aataatctac aacggactga aaaaggccga
     5461 aattgaagat atagagaaac gtataggcat acttaaggaa aagcaaatca caccgaatat
     5521 gtccgaccgt gcttcaaaat caattaagaa gtacctcgac gaaaagggat tttcgaatgc
     5581 agatattaag gtttatcaaa aagacgatcc cgcaaaaccg ggatttgtga aagtggagat
     5641 tgatgtggac aagaaattga aaacccgcgt acacaaaatt tatgttacag gaaatgaaaa
     5701 tctgaacgcc ctggagataa acaaagcgat gaagaagacg aatgataaca acattatcaa
     5761 tatcttcaga accaagaagt atgtaaagga cctctacgaa aaggataaaa ttgctgccat
     5821 cgaaaaatac aatgaattcg gataccgcga tgcatatatt gtttcagaca gcgttgttcg
     5881 ctttgatgat aagtcagttg atatttacct taaattcaac gaaggaaata aatattattt
     5941 ccgcgatata aaatggattg gaaataccat ctatccttac gaaacattaa atcaattcct
     6001 gacaataaaa aaaggcgatg tttataattt aaaactcctt aataaaagac ttcaggaaga
     6061 tgaagacgcg gttgcaaaac tgtattcaaa caagggttat ctgttcttca atatccagcc
     6121 tgtcgaagta gcaattgaaa atgattcgat tgactttgaa attcatatgg ttgaaggtaa
     6181 accagctacc atcaatgagg taaaaattac cggaaatacc cgggtttacg agaatgtagt
     6241 tcggcgtgaa ctccgaactg tacccggagc gctttacagc caggatgata tcatgcgtac
     6301 cttgcgtgaa ctggcacaaa tggggcactt tgatccggag aagttatcaa aagacatgat
     6361 ggaaggtggt ctgaagccaa atcctgaaaa cggaacggta gatctaacct ataaactgga
     6421 aacaaaaagc agcgaccagg tagaattctc ggccggttgg ggagctgcag gtattgtagg
     6481 aagtttagga ctgaaattca gtaattttgc cattcagaac atcttcaaac ctgaaaccta
     6541 ccgtattgtt ccgcaaggtg aaggacagac cttctcgatt aatgcccgaa ctaacggtaa
     6601 ttattattct tcgttctcac tctccttcct ggaaccctgg ctgggaggaa aacgaccgaa
     6661 ctcactttct gtttcactat actacacagc cacatcgggt atgagcgacc gctatctgaa
     6721 tgcatattca agtatgtatt ccagcatgta tagtggttat ggaggttacg gttatggagg
     6781 ttacggctat ggaggctatg gctcaggcta tggttacgga tcgggttatg gaaataccag
     6841 ttacgaaact gaagtggata aaaacaagta tttccgtact ctcggcgcat cggttggtta
     6901 tggaaaacga ttaagctggc ccgatgactt tttcactttc tatggtgagt tgtcgtttca
     6961 gcgttacatg atcaataact ggtatcagca ggaaacgaat gtaacaaacg gtgtattcaa
     7021 taatgtgggc ttgaatttga caatcggcag aaactcaatc gacaatccta tttttacccg
     7081 gaagggatca aatttctctc tcggactaca gataactcct ccgtactcta aattcaatgg
     7141 taaggattat tcgagttcaa gcatgactga tgccgaacga tacaaattcc tcgagtatca
     7201 caaatggaaa ttcagtg
//
LOCUS       DAOPYB010000158         7145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12767_length_7145_cov_10.105642, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000158 DAOPYB010000000
VERSION     DAOPYB010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12767_length_7145_cov_10.105642"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..339
                     /locus_tag="PKH58_10560"
     CDS             130..339
                     /locus_tag="PKH58_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89515.1"
                     /translation="MARNLIIHILLQRFSHTVAVFATALQNICSDLVTTEKQAPVGLN
                     ILINFNYSKLQKFLCFFVGQKWPDI"
     gene            complement(750..3017)
                     /locus_tag="PKH58_10565"
     CDS             complement(750..3017)
                     /locus_tag="PKH58_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX89516.1"
                     /translation="MKKIFIICVMILSYHISNAQNKITGKITDQDNLPMVGANIFVPD
                     MNKGTTSNNNGTYELSNLPNGKIKIQFSYIGYGNCVESVVLNGKSLELNIALTQTAIE
                     AEEVVVSGGYSSTQHENAVKIDVLKLDPRAIISTPNFTEVLTKVSGVDMISKGSGVSK
                     PVIRGLSMNDILVLNNGVRFENYQYSSHHPLGIDEFGIEDVEIIKGPASLLYGSDAIG
                     GVINFIKEKPAPIGSIIGDYNLRLFSNTLGMTNNLGIKGASKKFFGGIRVGQKINSDF
                     LQGGGAYVPNSRFNEMSVKANAGFTDKVGTFKLFYDFNNQKLGLVEDEAIEEITERGR
                     KNKVFYQELNTHLLSSQNKFYLGKFKLDINSALQNTELVHFGDVGVYEIQMELATLTY
                     DAKLHLPSKENSEYIIGFQGFNQTNTNLNDRETKLLPDATTNNYSAYGLLQYTFFKKL
                     KVQTGVRYDYKTMSTQAIGLATDTMIYRAPLDKSFGSFSGSLGATYNVSDKLLFRANF
                     AAAYRTPNLAELTSNGQHELRYEIGDQNLVPENAYETDLSLHYHKDNFTFDIAGFYNI
                     VNNYIFISPTDDTTPSGIDIYKYKQANSTLFGGEAGLHVHPKTLQWLHFEITFSSVIG
                     KQQNGDYLPFVPAHKLRFELRGEKEKLLFFKNAFVSVYTCSAFNQNNAAPDETTTAGY
                     TLIDLSIGGNIKMKNQSISLSLCANNILDKKYIDHLSTLKEVNLYNPGRDISLNLQIP
                     FGVKMNDKNSREIPE"
     gene            complement(3738..4169)
                     /locus_tag="PKH58_10570"
     CDS             complement(3738..4169)
                     /locus_tag="PKH58_10570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNX89517.1"
                     /translation="MIEHEYKFRVTYPDTDKMGTMHHANYVKYYEAARWELLRSIGVP
                     YASIEEVGVMCPVIRMDFKFIKSTRYDELLRVKTTLKAMKGVRLWFTYHLYNEQNELI
                     NEAETEIAFVGTTNWKPCPPPAFLLSAIHKYTDFQTLVGGI"
     gene            complement(4182..4559)
                     /locus_tag="PKH58_10575"
     CDS             complement(4182..4559)
                     /locus_tag="PKH58_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNX89518.1"
                     /translation="MKIAVPTKENNQIDDHFGHCEFYTIFLISDNNEIMAETILESPQ
                     GCGCKSNIASELAQMDVRIMLAGGIGDGAVNKLASQNIQVIRNCKGDVHQLVKDYLAG
                     NIQDGGSNCASHGHEEGHVCSHN"
     gene            complement(4566..4745)
                     /locus_tag="PKH58_10580"
     CDS             complement(4566..4745)
                     /locus_tag="PKH58_10580"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HNX89519.1"
                     /translation="MSRLNGKGPENKGSGTGRSLGNCCKNSEKNSTKYKLGQGMGKRR
                     KAGIIENKNQDNNYN"
     gene            complement(4757..5593)
                     /locus_tag="PKH58_10585"
     CDS             complement(4757..5593)
                     /locus_tag="PKH58_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX89520.1"
                     /translation="MKISILSDNRKIDETLDTEHGLSIYLETKRYKCLLDTGASDHFI
                     HNAEKLGVDIEAVDYVFISHGHADHIGGLTAFLKMNTKAKVVLSKNALTQRFYSNRNG
                     LKEISSELNLQNTENRFVFVDSETIFKDEIHVFPCQVCSNPMPKANKTLLKKSKNEII
                     ADDFNHELIVCFGKNEMIVYSGCAHCGVLNILDSVKQTIGKPISMVFGGFHLLDGYEG
                     QYETEFEINKLADELTSHYPETKFYTGHCTGEHVYKLLKDKLKDNLKLFYTGFTILGS
                     TN"
     gene            complement(5590..6006)
                     /locus_tag="PKH58_10590"
     CDS             complement(5590..6006)
                     /locus_tag="PKH58_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNX89521.1"
                     /translation="MPRPKQDRKISNPPLMQGFKPFGIPRRMLSSVSLLYDEYEAIRL
                     LDYEGMNQDQAAVQMNVSRPTLTRIYEKARKTIAQALVEGNMIVIEGGNVQFDREWFR
                     CRRCYKLVGGLENHVHCKDCTSFNDEELIPINNPDK"
     gene            complement(6162..6872)
                     /locus_tag="PKH58_10595"
     CDS             complement(6162..6872)
                     /locus_tag="PKH58_10595"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX89522.1"
                     /translation="METNEIISLPRIGDKAPSFKAVTTQGNINFPEDYAGKWAILFSH
                     PADFTPVCTSEFMTFASMQKDFDALNTQLVGLSVDGLYSHIAWLRTIKEKIEYKGMKN
                     VEVNFPLIEDITMEVANKYGMMQPGESNTKAVRAVFFIDPKGIIRAMIYYPLSLGRNF
                     DELKRVIIALQTADNFGVAMPADWRPGDDVIVPTAGSCGVAKSRMEGEEEGVKCYDWF
                     FCTKELSKDKVEAELGKK"
BASE COUNT         2114 a         1363 c         1169 g         2499 t
ORIGIN      
        1 tccggtcaac agtgattctt aataaaataa tatttcacac gattaacgat taaactcaat
       61 attgttaatc actattgtcc gataaaattt aatatttagc cacttccgat ggatcggaat
      121 gttccatcta tggcaagaaa tcttattatt cacatcttac tacaaagatt tagccacact
      181 gtggctgttt ttgcaacagc gttgcaaaat atttgtagcg atttggtaac aacagaaaag
      241 caagccccgg tggggcttaa tattcttatt aatttcaatt atagtaaatt acaaaaattt
      301 ctgtgttttt ttgtcggaca aaaatggcca gatatataat gtcagttcct taggtaaatt
      361 caaaaaagtc atgacgactt cactgacaaa actggcgttg aaagaataaa gataaataat
      421 tctggtaacg aactgtataa aaagaaaact gctgaacttc aaaggaagct cagcagttgc
      481 tatatggttc aattcacagg tctatttatc tgtttctttc agtttcacaa aatacatggc
      541 aaaggagcgg tacaggaagt gtgttccacc cttacggata ttctctttca ttgctgaaat
      601 ttcacaccgg cttctttatt attcgaattt taatccggaa tataattcca tatagaatgt
      661 tgagaccccg catgacacgt ctccactaat cccgacgtcc agcgcggtta tttactcatt
      721 tatcggcata tcctacgatt cggaactgtc tactcgggta tttctcgtga atttttgtca
      781 ttcattttca ctccaaatgg aatttgcaaa ttcaatgaaa tatctctacc cggattatat
      841 aaattcactt cttttagtgt cgataaatgg tcaatgtatt tcttgtcaag tatattgttt
      901 gcacataaac ttaacgaaat agattgattt ttcattttta tattacctcc aatgcttaaa
      961 tcaataagcg tataacctgc agttgtagtc tcgtcaggtg ctgcgttgtt ttgattaaat
     1021 gcggagcatg tatatactga aacaaaagca tttttaaaaa acaataactt ttctttttcg
     1081 ccccttaatt caaaacgcaa tttatgagca ggcacaaatg gcaagtagtc accattctgt
     1141 tgttttccga ttactgatga aaacgtgatt tcaaaatgca accattgtaa tgtttttgga
     1201 tgaacatgta aacctgcttc acctccaaac aaagtagaat ttgcctgttt gtatttatat
     1261 atatctatcc ccgaaggtgt agtgtcatct gttggtgaaa tgaaaatata attattgaca
     1321 atgttgtaga atcctgcaat gtcaaaggtg aaattatcct tatggtaatg aaggcttaaa
     1381 tccgtttcat aggcgttttc gggaacaaga ttttggtctc cgatttcgta tctcaattcg
     1441 tgttgtccgt ttgaagtaag ttctgccaaa tttggtgttc ggtaagctgc tgcaaaattt
     1501 gctctaaata gtagtttgtc agatacatta taagttgcac cgagcgaacc gctgaaactt
     1561 ccaaatgatt tgtcaagtgg tgcacgatat atcattgtat ctgtcgctaa cccaatagcc
     1621 tgtgttgaca ttgtcttata atcgtaacga acaccagttt gtactttaag tttcttgaaa
     1681 aacgtatatt gcaacaaacc ataagcagaa taattattcg ttgtagcatc aggcagtaat
     1741 tttgtttctc tgtcattcag gtttgtattg gtttgattga atccctgaaa cccgataata
     1801 tattctgagt tttccttcga tggcaaatga agtttggcat cataagttaa ggttgcaagt
     1861 tccatttgta tttcatacac accaacatcg ccaaaatgaa ctaattctgt attctgtaac
     1921 gctgaattaa tatcaagttt aaatttgcct aaataaaact tgttttgaga agaaagtaaa
     1981 tgtgtattta attcctgata gaaaactttg tttttacgtc ctctttcagt aatttcttct
     2041 attgcctcat cttcgacaag tccaagtttt tgattgttga agtcataaaa caatttgaaa
     2101 gtaccaacct tatcagtaaa tcctgcattt gccttaacgg acatttcgtt aaagcgtgaa
     2161 tttgggacat aagcgccacc tccctgaaga aaatcagaat ttattttttg tccaactctg
     2221 atacctccaa aaaacttttt cgatgctcct tttattccta aattattagt catgcccagc
     2281 gtatttgaaa aaagtcttaa attgtaatcg ccaataatac tgccaatcgg agcaggtttt
     2341 tcttttataa aattgataac accgccaatt gcatctgaac cgtaaagtaa tgaagcaggt
     2401 cctttgatga tttcaacgtc ttcaattcca aattcgtcaa ttccgagtgg atgatgactt
     2461 gaatattggt aattttcaaa tcttactcca ttatttaaaa ctaaaatatc attcattgaa
     2521 agcccacgaa ttacaggctt tgaaactcca cttcctttag aaatcatatc aacaccagac
     2581 acctttgtaa gtacctctgt aaaatttggg gtacttataa ttgctcgcgg gtcaagcttc
     2641 agaacatcaa tctttacagc attttcatgt tgtgttgagc tatatccacc agagacaaca
     2701 acttcttcag cctctattgc cgtttgtgtg agagcaatat ttaattctaa acttttccca
     2761 ttaagaacca cagattcaac acaattcccg tacccaatat atgaaaattg aattttaatt
     2821 ttgccattcg gtaaattcga caattcatac gttccattat tatttgatgt agttccttta
     2881 ttcatgtcgg gaacaaaaat gtttgcacca accatgggta aattatcttg gtcagtaatt
     2941 tttcctgtta ttttgttctg tgcgtttgat atatgatagg acaatatcat aacgcaaatg
     3001 ataaatattt ttttcatttc attacgtgtt aatttattaa tcgaatttct catataacac
     3061 atcaacaggt gtttgcttca aaatctgtct atatggcggt gcacgtaata aaaatgaata
     3121 tttagagaat gtagaataat taacagacct gtaattattg caatacttcg ctacaggttg
     3181 ctcttttgtt aatactattt tttttaagtc tgacgagaaa acagaaaatt caaaattaca
     3241 aacagcacat ttttcatgaa atacatgttt atgcttctca ttctttgcat tgcattcaac
     3301 gtgttcatga tgatgctcaa gtttaactat tgaaggtaag aggaatacca acaacagtaa
     3361 taaagaagta atattattaa atttcctaat catcaaactg tttgtcctta cctgtaggtg
     3421 tcgtttttaa atgagctcaa acgagagttt gctcaaaaac ctgtactctc gcgtttttct
     3481 tgtttatata ttattctatg cttcgatagc tcccatttat ggaagtaaat ttgacgatgc
     3541 cgattgtaag cgacaaacgc caaaattact tcgccaacac cttcaaccga tcttactgat
     3601 attctctttt attgcttaaa cattaacacc cgtgccgatt tcgaagactt tcggaatcgg
     3661 cttctttatt attcgaatta ttaaatccgg agtattattc cataaagaat gttgagaccc
     3721 cgcataatac gccactacta aatcccgccg accagcgttt ggaaatccgt atatttatgt
     3781 attgcggaaa gtaaaaatgc aggcggtgga cagggtttcc agttggtcgt tcccacaaat
     3841 gcgatttcag tttcagcttc gttgatcagt tcgttttgtt cgttgtacag atgataggta
     3901 aaccacaaac gtacgccttt catagctttc agggttgttt tcacccttaa aagttcatcg
     3961 tatcgggtag atttgataaa tttgaaatcc attcgtataa ccgggcacat cacaccgact
     4021 tcttctattg aagcgtaggg cactccgatg ctgcgaagta attcccagcg agcagcttcg
     4081 taatatttca cgtagttggc atgatgcatt gtccccattt tatcggtatc ggggtaagta
     4141 actctgaact tatattcgtg ctcaatcata aaccatacag tttagttatg gctacaaacg
     4201 tgtccttctt catggccatg tgaagcacaa tttgaaccgc catcctgaat atttccagcc
     4261 agataatctt ttactaattg atgtacgtca cctttacaat tacggataac ctgtatgttt
     4321 tgtgaagcca gtttattgac tgctccatct cctattcctc cggcaagcat tattcttaca
     4381 tccatttgtg ccagttctga agcaatgttt gacttacaac cgcaaccttg tggagattca
     4441 agtatggttt cggccatgat ttcgttattg tccgaaatga gaaaaatggt gtaaaattca
     4501 caatgaccga aatggtcgtc aatttggttg ttttcttttg ttggtactgc tattttcata
     4561 ttgtattaat tataattgtt gtcttgattt ttgttttcaa taattcccgc tttgcgtcgt
     4621 ttccccattc cttgtcccaa tttatatttt gtagagtttt tttcggagtt cttacagcag
     4681 tttcctaaac tgcgacctgt ccccgaacct ttgttttcag gtccttttcc atttaatcgt
     4741 gacataattt tagaatttag ttcgttgatc ccaatatcgt aaaaccagta taaaacagtt
     4801 ttaaattgtc ttttaatttg tctttcaaca gcttatatac atgttcgcct gtacaatgtc
     4861 ctgtgtaaaa tttagtttcg ggataatgtg aagtcaattc atcagctagt ttgttaattt
     4921 caaattctgt ttcatattgt ccttcatatc catcaagcaa atggaatcca ccaaaaacca
     4981 tagaaatagg ttttccaata gtttgcttta ccgaatctaa gatatttaag acaccgcaat
     5041 gagcacagcc agaatataca atcatttcat tcttaccaaa gcaaacaatt aattcatgat
     5101 taaaatcgtc ggcaataatc tcattttttg acttctttaa tagtgtttta tttgctttcg
     5161 gcataggatt actacacact tgacacggaa aaacatgaat ctcatctttg aatattgttt
     5221 ctgaatcgac aaacacaaaa cggttttctg tgttttgtag gtttaattcg ctacttattt
     5281 cctttaatcc attacggttt gagtagaaac gttgtgtgag tgcattcttt gacaaaacaa
     5341 cttttgcctt tgtattcatt ttcaggaatg ccgtcaatcc tccgatatgg tcagcatgtc
     5401 cgtgtgaaat aaagacataa tcaaccgctt caatatcaac tcccaatttt tcagcattgt
     5461 gtataaaatg atcagatgca cctgtatcga gcaaacattt atagcgtttt gtttccagat
     5521 aaatacttaa tccatgctca gtgtcaagtg tttcatcgat ttttcgattg tcagataata
     5581 tcgaaatttt catttatccg gattatttat cggaattaat tcttcatcgt tgaaggatgt
     5641 gcaatcttta caatgtacgt ggttttccag tccgcctaca agtttataac agcgtcggca
     5701 acgaaaccat tctctgtcaa actgcacatt tcctccctct attactatca tattcccttc
     5761 tacaagtgct tgtgctattg ttttccgggc tttttcataa attcgggtca acgttgggcg
     5821 cgaaacattc atttgcacag ctgcctgatc ctggttcatc ccttcgtaat cgagaagccg
     5881 gattgcttca tattcatcgt aaagcaatga cacagaactc agcattctgc gggggatgcc
     5941 aaaaggttta aatccctgca tcaatggagg attggatatt tttcggtctt gtttcggacg
     6001 gggcataatt ctgattttag gtaatgaatt tatttggttt gtgaatgaca aagataaaga
     6061 aataattatg aacttatgtt cgatttattg aaaaatttat tcacaaaaaa agggaaaaac
     6121 agtgtttacg tttctccctt tcttatcagt tggtttactt tttatttttt acccagctct
     6181 gcttcaactt tgtctttcga caattctttt gtacagaaga accagtcgta gcatttcact
     6241 ccttcctcct cgccttccat acgacttttt gcaactccgc atgaaccggc agtaggtaca
     6301 attacatcat cacccggacg ccagtcggca ggcattgcta caccaaaatt atcagcagtt
     6361 tgaagagcaa taatgacacg tttcagctcg tcaaaattac gtcccagcga aagcggataa
     6421 taaatcatgg cgcggattat tcctttgggg tcgataaaaa atacagcacg aactgctttt
     6481 gtgttgcttt cgcccggttg catcattcca tatttgttgg ccacttccat ggtaatgtct
     6541 tcgataagcg ggaaatttac ttccacgttt ttcattcctt tgtactctat tttttcttta
     6601 atggttctca accaggctat gtggctgtat aatccatcaa ccgaaagacc cactaactgt
     6661 gtattaagtg catcgaaatc tttttgcatc gaagcaaaag tcataaattc agaagtacat
     6721 accggcgtaa agtcggccgg atgactgaat agaatagccc acttgcctgc atagtcttcg
     6781 ggaaaattaa tgttaccctg agtggttact gctttgaaag aaggtgcttt gtcgccaata
     6841 cgcggcaatg aaataatttc gtttgtttcc attttcttga ttgtttttgt ttattattta
     6901 ttttataaat ttatgttcat gaattttttt ttacaaaaat tgaaactaaa atatttctat
     6961 atcatatcta tttaatcgac atctaaattg accctactta tatttattgg atcaatctca
     7021 aatttatttt atcgaactta aacttaaatt taaaacaaat aaaatgaact tatgttcact
     7081 aacaatttga ttttaacaaa aaaaaagaga aacttctttt gtttctcctt ttttgaaatt
     7141 tataa
//
LOCUS       DAOPYB010000159         7129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12811_length_7129_cov_9.140373, whole genome shotgun sequence.
ACCESSION   DAOPYB010000159 DAOPYB010000000
VERSION     DAOPYB010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12811_length_7129_cov_9.140373"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1178
                     /locus_tag="PKH58_10600"
     CDS             372..1178
                     /locus_tag="PKH58_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX89523.1"
                     /translation="MDRLRFRSLASGSSGNCYFLGTASYGILIDAGIGIRTIRHFLRT
                     MGLDFSNIWGLFITHDHADHIRAAGVIGEKHKVPVYTTKLIHNGINKSYCVTQKLSTS
                     QKFIEINEIVDIGDFRIKAFPVSHDATENVGYNIEFKGKSFTFATDLGYVSLEAAEQL
                     VNSDFMVLEANYDDFMLENGPYPLHLQRRIKADTGHLSNLQTARFLTENFTDRWKYVF
                     LCHLSRENNLPELAYSTIVESLRNEEIDFEDKLQIVPLDRINPSPMYLFD"
     gene            1229..1969
                     /locus_tag="PKH58_10605"
     CDS             1229..1969
                     /locus_tag="PKH58_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNX89524.1"
                     /translation="MPSKLVIIPTYNEKENIENIIRAVFALKEEFHLLVIDDGSPDGT
                     AAIVKNLRIEFPERLFIVERKGKLGLGTAYIAGFKWGLEHKYDFIFEMDADFSHNPLD
                     LPRLYNACINEGADVAIGSRYVSGVNVVNWPIGRVLMSYFASKYVKLVTGLKIADTTA
                     GFKCYRREVLETIELDKIRFKGYAFQIEMKFTAYKCGFTLKEVPIIFINRQLGTSKMS
                     GGIFGEAFLGVIQLKLSSLTRKFPRKMI"
     gene            2020..3366
                     /locus_tag="PKH58_10610"
     CDS             2020..3366
                     /locus_tag="PKH58_10610"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNX89525.1"
                     /translation="MMKKLICNANIVNEGQIFKGTVLINHDKIEHVYRDGEQIELKNP
                     VEKIEAEGLWLMPGVIDDQVHFREPGLTYKGDIYTESKAAVAGGVTSYMEMPNTKPQT
                     TTLELLEEKNELAASKSLANYSFYLGATNMNIRDLIKVDPGKICGVKVFMGSSTGNML
                     VDDLEILNRIFAEVPALIAVHCEDEPTILKNLNYYKNIYGENIGCEFHPLIRSEEACY
                     KSSSFAVELAEKHGTRLHVLHLSTAREMSLFRNDIPLKDKKITAEACVHHLWFSSNDY
                     ARYGSRIKWNPAIKTEADRLALIEAVNNGKIDVVATDHAPHAQDEKKGNCYKSYSGGP
                     LVQHSLPAMLQLARQGYFSIEKVVEKMCHAPADLFHVHRRGYIRPDYYADLILVDPQS
                     TQLVTAQNLLYKCGWSPFEGTTFDAAVVTTFVNGIKVFDRGEWNEDHKGKRLTFDI"
     gene            3399..4688
                     /locus_tag="PKH58_10615"
     CDS             3399..4688
                     /locus_tag="PKH58_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNX89526.1"
                     /translation="MEIIILLILILFNGFFSLSEIALVSSKETRLEHYKQNGNKGALT
                     ALKLRSDSEKFLSSVQVGITLISLITGAYGGMSLAEDVAPLIARIENLRPYAPGISMV
                     IIMFLITYVSIVIGELVPKTVALSNPEKVAVRVAPIINFFSKLFYPFVKLLSSSTTFI
                     TKMMGVEKQTEHLTESELRQMIKTASIEGVIEKEQNEIHEKVFYFSDKKAKHLMTHRM
                     EVEWVDINAPEDEIRKELDQCQHSKIVCCHETIDQIEGVLLQIDYYKALAEGKDFRVR
                     DLLKEPIVVHENVLAPKVLQNLRQNNTHFSIVVDEYGDFEGIITLHDVMENIVGYILD
                     EGEVEEPDYFVREDNSVLVNGDAPIEVLMEILDDFYVDYDQIDYTTVAGFVIDQINVI
                     PKTGDKIIYLDYNIEIMDMDGNRIDKVLISRIKDENE"
     gene            4711..5724
                     /locus_tag="PKH58_10620"
     CDS             4711..5724
                     /locus_tag="PKH58_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008018671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNX89527.1"
                     /translation="MKIKFFPAILPLVCLLFLTQCKPVNKEYTFLEGKIFGTFYHITY
                     LHPKGVDLTKEIEERLKEFDASLSTFNPNSIISRINRNDSDVNTDIFFEEMYSMSRQV
                     SIKSNGAFDITVAPLVNAWGFGYGNHDTSLKPNLDTIMPYIGYQKIKLLNHKMIKDDP
                     HIMIDASAIAKGQGSDVIARLLEEYGCENYMVEIGGEIACQGLNPKGEKWHIGIDKPA
                     DDPADENKELQTIIAISNVGMATSGNYRQFYFKDGKKYAHTINPHTGYPVQHNLLSAT
                     VIAPTCMQADAYATAFMVLGVESSMSVCSTIPDMDCYLIYADKNGEYKTVYTKGFQKY
                     LTE"
     gene            5821..6624
                     /gene="bamD"
                     /locus_tag="PKH58_10625"
     CDS             5821..6624
                     /gene="bamD"
                     /locus_tag="PKH58_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNX89528.1"
                     /translation="MKKYIFPIILSMFLFASCSEYQKLLKSTDPELKYDKAVEYFTAK
                     KYTKAITLFDEVSPYYKNTDRSELILNFLAQSYIEKKDYYSASEYYKTYIKSYPRGRY
                     IQEARFGVAYCYYKDSPDVRLDQENTNLAISSFQEFIDLYPENERVKEAIKLLDELND
                     KLALKGLTNATLYYNLGTYLGRNNYLAAVIEAENTLKKYPATKHREDLMIMILKSKYQ
                     QALLSYAETKVDRYQSAIDECYNYINEYPAGKFRKNTDEILKVSENNIK"
     gene            6647..6979
                     /locus_tag="PKH58_10630"
     CDS             6647..6979
                     /locus_tag="PKH58_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNX89529.1"
                     /translation="MDYKKLNAPSSTITRDMNSLCENVGNVYETVSIIGKRSNQIDVE
                     LKVELDKKLQEFASYSDSIEEVFENREQIEISRFYEKMPKATLLATQEFIEDKVFYRN
                     PLKDKLKK"
BASE COUNT         2283 a         1331 c         1478 g         2037 t
ORIGIN      
        1 ctgtggctac attacccaca ttgtatgatt atattatcaa taaccagctc gaaggacatg
       61 aactcaatag tggagacttg gtagtgtttg cttccgttgg tgcaggtatg agtattaata
      121 gttttgtgta taaaatgcca taattggtta ttattgatat aatgtcgccc ggaaatgaaa
      181 gtttccgggt gcgttttttt aaatgacatc taaaacaacc tgtaatacat ttctccgggt
      241 tgattttaac ctgattcttt tatttctttt caatgttttt tttgaaacgg tttcttttaa
      301 agatatttcc tcaccaatat aaatgttatt acgttttttg tgttaatttt gtaactaatt
      361 tgaataggaa tatggataga ttgcgattca gaagtctggc aagcggtagc agtggtaatt
      421 gttattttct tggaactgca tcatacggga tactgattga cgcgggtatt ggaattcgta
      481 ctatccggca ttttttacgt accatgggcc tcgatttctc aaatatctgg ggtttgttta
      541 tcacacacga tcatgccgac cacattcggg cagccggagt aatcggggag aagcacaagg
      601 ttcctgttta tactaccaaa ctgatacaca atggaattaa taaaagctat tgtgttaccc
      661 agaaactgtc aaccagtcag aagtttattg aaatcaatga aattgttgat attggcgatt
      721 tcagaattaa agccttcccc gtatcgcacg atgctacaga aaatgtaggg tataacatcg
      781 aatttaaagg aaaaagtttt acatttgcta ccgaccttgg atatgtgagt ttggaagcag
      841 ctgaacagct ggtaaattcg gattttatgg tgctggaagc caactatgat gattttatgc
      901 ttgaaaacgg accctatcct ttacatctgc aacgcaggat caaagcagat acgggacacc
      961 tgagcaatct ccagacagca cgttttctga ccgaaaattt taccgatcgc tggaagtatg
     1021 ttttcctttg tcacctgagc agagaaaata acctgcccga actggcatac tctaccatcg
     1081 tcgaaagtct gagaaacgaa gaaattgact tcgaggacaa gctacagatt gttcctttgg
     1141 acaggataaa tccatcgccg atgtatttgt ttgattagaa ctgaaatatt atctgttaat
     1201 acgataaaaa actaaaaata atctttttat gccttcaaaa ctggttatca ttcctactta
     1261 caacgaaaag gaaaacattg aaaacatcat tcgtgcagta tttgcactca aggaagagtt
     1321 tcacctgctg gtaattgacg acggctcacc cgatggaacg gcagctatcg taaagaacct
     1381 ccggattgaa tttcccgaac gcttgtttat tgtggagcgg aaaggtaaac tgggattagg
     1441 tacggcctat attgccggtt tcaaatgggg tttggagcat aaatatgatt ttattttcga
     1501 aatggatgct gatttttccc acaatcccct ggatttgccc cgcttgtata atgcctgcat
     1561 caacgaaggc gccgatgtag ccatcggatc gaggtatgtg agcggtgtca atgtggtaaa
     1621 ctggccaatc ggacgggtgc tgatgtcata ttttgcatcc aaatatgtaa aacttgtcac
     1681 aggcttaaaa attgccgata ctactgccgg ttttaaatgt tatcgccgtg aagtgctcga
     1741 aaccatcgaa ctggacaaaa tccggtttaa gggttatgcc tttcagatcg aaatgaaatt
     1801 tacagcatat aagtgcggtt ttactctcaa agaggtaccc attatattca tcaatcgtca
     1861 gctgggtact tcaaaaatga gcggtggtat attcggagaa gcatttttgg gggtaataca
     1921 actgaaactg tcttccctta cccgtaagtt tccgcgaaaa atgatataaa taactattat
     1981 tttacatact ttcgtttatt tacaccttta aataatcgca ttatgaaaaa attaatatgc
     2041 aatgccaata ttgtcaacga aggacaaatt tttaagggta cggtattaat caatcatgac
     2101 aagattgagc atgtttacag ggatggcgaa caaatcgaac tgaaaaatcc ggttgaaaaa
     2161 atcgaagcgg aagggttgtg gcttatgccg ggtgtgattg atgatcaggt acattttcgc
     2221 gaacccggtt tgacttataa gggcgatatt tatactgaaa gtaaagccgc ggtggccggg
     2281 ggtgtcacct cctatatgga aatgccaaat acaaaacccc aaaccactac gctggaactt
     2341 ctggaagaaa aaaatgaact ggcagcctca aaatcgctgg caaattattc cttctacctg
     2401 ggtgccacca acatgaatat ccgcgatcta attaaggtgg atccggggaa aatatgcggt
     2461 gtaaaagtgt ttatgggatc atcaaccggc aacatgctgg tcgacgatct ggaaatactt
     2521 aaccgtattt ttgcagaagt tcccgccctt atcgctgtac attgcgaaga cgaaccaaca
     2581 attcttaaga atctgaatta ctataaaaat atttacggag aaaatattgg ttgtgagttt
     2641 catccgctga tacgttccga agaagcttgc tacaaatcat cttcctttgc agtggagttg
     2701 gccgagaagc atggtacacg cctgcatgtg ctgcatttgt ccactgcccg tgaaatgtcg
     2761 ctgttcagaa atgatatccc gttgaaagat aagaaaataa cagccgaagc atgtgtacat
     2821 catttatggt tttcgtccaa tgattatgct cgttatggaa gtcgtattaa gtggaatccg
     2881 gctataaaaa ctgaagctga ccgcttagca ctcatcgaag ctgtgaataa cggtaagatt
     2941 gacgtagtag ccacagatca tgctcctcat gctcaggatg aaaaaaaagg taattgctat
     3001 aagtcatatt cgggaggacc acttgttcag cattcacttc ctgcaatgct tcagcttgcc
     3061 cgtcagggtt actttagtat cgaaaaagtg gtagagaaaa tgtgtcatgc accggcagac
     3121 ctgtttcacg ttcaccgcag aggatatatc cggcctgact attatgccga cctgatactc
     3181 gtcgatcctc aatcaacaca actggtgacc gcgcaaaatc tactttataa atgcggatgg
     3241 tcgccatttg aaggaacaac ttttgatgcc gccgtagtca ctacttttgt taatggtata
     3301 aaagttttcg acaggggcga atggaacgaa gatcataaag gaaaacgtct gacttttgat
     3361 atatagtgtt tgtctgttaa taatcctgtg aaactataat ggaaataatt attttactga
     3421 tacttatatt atttaacggt tttttttcgc tttcagaaat agcattggtt tcgagcaagg
     3481 aaacccggct tgaacattac aaacagaatg gtaacaaggg agcactgaca gccctgaagt
     3541 tacgatcaga ttctgaaaaa tttctttcat ccgttcaggt gggtattaca ttgatcagcc
     3601 ttattaccgg agcatatggt ggaatgagcc tggctgaaga tgtagctcct ttaattgcca
     3661 ggatcgaaaa tctgagacca tacgcgccgg gtatttccat ggtaataatt atgtttctta
     3721 taacttatgt gtcaattgtt ataggtgaac ttgttccaaa aacagtagcg ctgagtaatc
     3781 ctgaaaaagt agccgtgagg gtggctccga tcatcaattt ctttagcaaa ctgttttatc
     3841 cctttgtgaa attgctatcg tcttctacca catttattac caaaatgatg ggagtggaga
     3901 aacaaacgga acatcttaca gaatcggagt tgcgtcaaat gataaaaaca gcttccattg
     3961 aaggagtcat cgaaaaagaa caaaatgaaa tacatgaaaa agtgttttac ttttctgaca
     4021 agaaagccaa acacctgatg actcaccgaa tggaggtgga gtgggtcgat ataaatgcac
     4081 ccgaagatga aatcagaaaa gagttagacc aatgtcagca tagtaaaatt gtttgttgtc
     4141 atgaaactat tgatcagatt gaaggagtac tcttacagat tgattattat aaagcattgg
     4201 cggaagggaa ggatttcagg gtacgcgatt tgctcaaaga acccattgtg gttcacgaaa
     4261 atgtgctagc acctaaggtt ttgcagaatc tacgtcagaa caacacacat ttcagcattg
     4321 tagttgatga atatggcgat tttgaaggaa ttattaccct gcacgatgtt atggaaaata
     4381 tcgtgggata tatactcgat gaaggagagg tcgaggagcc cgattatttt gtgcgtgaag
     4441 ataattcggt actggtcaac ggcgatgctc ccattgaggt gctgatggaa attctcgatg
     4501 atttttatgt ggattatgat cagattgatt atacaacagt tgccggtttt gtaatcgatc
     4561 agatcaatgt aatacccaaa acgggagata aaatcatata tctcgattat aatattgaaa
     4621 taatggatat ggacggcaac aggatagaca aagtgcttat ttccagaata aaagatgaga
     4681 atgaataaca aaatgactaa ttactagaaa atgaaaatta aattttttcc tgcaatactc
     4741 ccgctggttt gtctcttatt tctaacacag tgcaagcctg taaataaaga atatactttc
     4801 ctcgaaggaa aaatctttgg cactttttat catattactt atctgcatcc taaaggagta
     4861 gatctgacaa aggaaattga agaaagactc aaagaatttg atgcatcgct gtcgaccttc
     4921 aatcctaact ccatcatttc gcgcatcaac cgcaatgaca gcgatgtaaa caccgatatc
     4981 ttcttcgaag aaatgtattc catgtcgcgg caggtatcca ttaaatctaa cggagctttc
     5041 gatattaccg ttgctccgct tgtcaatgcc tggggctttg gatacggcaa ccacgacacc
     5101 tcacttaaac ccaacctgga taccataatg ccttatatcg gctatcagaa aattaaactc
     5161 ctcaatcata aaatgattaa ggatgatccg catattatga tcgatgcaag tgctattgcc
     5221 aaaggacagg gttcggatgt tattgcccgt ttgctggaag aatatggttg cgaaaattat
     5281 atggtggaaa taggagggga gattgcctgt cagggattaa atcctaaagg tgaaaaatgg
     5341 catatcggaa tagataaacc ggccgatgat ccggctgacg aaaataaaga actgcaaacc
     5401 attattgcaa tcagcaatgt agggatggcg acatcgggta actacaggca gttttatttc
     5461 aaagacggca aaaaatatgc tcacaccatc aatccgcaca ccggctaccc cgtgcaacac
     5521 aacctgctaa gtgccacagt gatagccccg acctgtatgc aggctgatgc ctatgcgacc
     5581 gcttttatgg ttttaggagt ggaaagtagt atgtctgttt gcagcacaat acccgacatg
     5641 gactgttatc tgatctatgc ggataaaaac ggagagtaca aaactgttta caccaaaggc
     5701 tttcaaaaat acctgacaga atgattgtat ctggctggaa actaacttca ttatcataaa
     5761 aaagataaat ttattttgta ttcctctcaa aataactaac tttgcaggct aaaattcggt
     5821 atgaaaaaat atatttttcc cataattctg agcatgtttc tattcgcctc ttgcagcgaa
     5881 tatcagaaat tgctgaaatc aacagatcct gaactcaaat acgacaaagc tgtggagtac
     5941 tttaccgcta aaaagtacac caaagctata accctgtttg atgaagttag tccctactac
     6001 aaaaatacag accgttcgga actgatactc aatttcctgg ctcagtcata tattgagaaa
     6061 aaagattact attcagccag cgaatactat aaaacttaca tcaaatcgta tccacgcggt
     6121 cgttacatac aggaagcacg tttcggagta gcgtattgtt attacaagga ttcgcccgat
     6181 gtaagactcg atcaggaaaa taccaatctg gctatcagtt cgtttcagga attcatcgat
     6241 ttatatcccg aaaacgaacg ggtaaaagaa gctatcaaac tgttggatga actgaatgac
     6301 aaactggctt tgaaagggct aaccaatgca acattgtact acaatctggg tacttatctc
     6361 ggtcggaata attatctggc tgcagtgatt gaagccgaaa atactctgaa aaaatatcct
     6421 gctaccaagc atcgcgaaga tctgatgatc atgatattaa agtcgaagta tcagcaggcg
     6481 ttgctgagtt atgccgaaac taaagttgac cgctatcagt cggcaattga tgaatgttac
     6541 aattatatca atgaatatcc tgccggtaag ttcaggaaaa ataccgatga aatactgaaa
     6601 gtgtccgaaa acaacataaa ataaaatcga aaaacgagtt attaatatgg attacaaaaa
     6661 attaaatgca ccatcgtcga ctattacccg cgacatgaac tcattatgcg agaatgtggg
     6721 taacgtgtat gaaacagttt ctattattgg caaacgttcc aaccagattg acgttgaatt
     6781 gaaggtagaa cttgataaga aacttcagga atttgcatca tacagcgaca gcatcgaaga
     6841 agtttttgaa aacagagaac agattgaaat atcccgtttc tatgaaaaaa tgccaaaagc
     6901 gactttgctt gctacacagg aatttatcga agataaagta ttctatcgca atccactgaa
     6961 ggataaactg aaaaaataat ctactgtaaa aataaacagt aaaaacgtac attaaaaaaa
     7021 ctgtttcttg tcaccgctaa ccggaggcag gaaactttta ttttaaaata actcgttgtt
     7081 agctggtttt ttgtattttt gtggagccat caactttcac gaactggta
//
LOCUS       DAOPYB010000160         7099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_12884_length_7099_cov_8.148779, whole genome shotgun sequence.
ACCESSION   DAOPYB010000160 DAOPYB010000000
VERSION     DAOPYB010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12884_length_7099_cov_8.148779"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1577)
                     /locus_tag="PKH58_10635"
     CDS             complement(135..1577)
                     /locus_tag="PKH58_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNX89530.1"
                     /translation="MNSRHIIIIFLLFPFFYFNANADVTLPKVISSGMVLQRDAKVPV
                     WGKAEAGEKVTVSFAGQEVTTVADADGKWSVTLKSLKLNVQPADMIISGKNTITLENI
                     LVGDVWLCSGQSNMEYPFDYRLKKYATPVRGENVALEELLKPKSDKIRYLYAEKQNDT
                     KDIKSVGWFTSNDSVLRNVSAIGYFFGKYIYEETNVPVGIISSSWGGTMIEEWTPAWV
                     YEKSPVFAGQIDPNDKKINGRIAGTKFYSMIQPIVPFAVKGMLWYQGETNCMSEDQAT
                     YPEKMKMLLETYRSLFRNPQMPFYYVQIAPYLYSKRTNDKMQHTPELLPEFWEAQSSF
                     LSTHRTGMVVTTDLVDNLSDIHPSYKWEVARRLSLWALAKDYNKKVIYQSPGYKSMKI
                     KDGKIILIFHNIGGGLISKDGEALTWFTIAGADGNFVPAKAEIKRKTVVVWADEVKQP
                     EMVRFAWNERAMPNLCNKKGLPVMPFRTGK"
     gene            complement(1590..1757)
                     /locus_tag="PKH58_10640"
     CDS             complement(1590..1757)
                     /locus_tag="PKH58_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX89531.1"
                     /translation="MKHTFRNLKNFGLFILFLLPVSTTVERDKNHPCILIRSIGNENP
                     FTPLYNSYDQN"
     gene            complement(1926..3344)
                     /locus_tag="PKH58_10645"
     CDS             complement(1926..3344)
                     /locus_tag="PKH58_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HNX89532.1"
                     /translation="MRRNIIFVLLLLSVVLVNAQNQAVAWKKIYPAIQKNIKAPVFRK
                     AQFDIRDFGAVANDTTVLNTEAINRAIVTCSLRGGGMVIVPAGVWFTAPLTLKSNVNL
                     HVQEGATLLFTTNPKYFPVVLTRWEGMDCYNLQPLIYAYGETNIAITGKGTLDGASSN
                     ENWWKKCGAASFGWKEGDISQRTGRPKLMQWNNDKVPVSMRIMNENDGMRPQMINLYL
                     CNNVLIEGVKILRSPFWVIHPLMCNNVIVRGVRVENDGPNGDGCDPEACSNVLIENCY
                     FSTGDDCIAIKSGRNNDGRLWSQPSENIIVRNCEMKNGHGGVVIGSEISGGYKNLFVE
                     NCRMNSPLLDRVIRIKTNTLRGGTIQNIYVRNIEVGVCKEAILHISLLYEPREIGERG
                     FHPVVQNINLEYIRSQKSQYGVSIDALPDTTCVRDIFVRNCNFNGVKSGNKLTGKIEN
                     VKFDNLTVNGKLMETYPLKKKN"
     gene            complement(3446..5554)
                     /locus_tag="PKH58_10650"
     CDS             complement(3446..5554)
                     /locus_tag="PKH58_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89533.1"
                     /translation="MKTKLFLFVIIFFISCGVSGKSGIDRYKLVSRNNPRLTAVDTLG
                     ALTVGNGEFACTVDITGLQTFQKDYSKGMPLGTQSQWGWHSFPNVKAYKPEETLKNFE
                     FGRGKAEPYAVQFNEKGRQQEAADYFRANPHRLHLGIVGLELEGAALADIKNPVQTLD
                     LWNGAIRSRFEVKNTMYEVLTATHPAADQITARVSTRGEKPAIKFQFPYPTGAHTDDA
                     CNWNAADKHSSVIVRKTENSALIKRQLDSTVYFVNITWENKARLVEKSKNYFVLQSDG
                     NELSFTCRFTTVQPENFNTGSEQTFAAASKFWNHFWKDGAAVDFSECTDPRAKELERR
                     VVLSQYLLAVQCSGTTPPQETGLTYNSWYGKFHLEMIWWHQAQFALWNRPEMLEKTLG
                     WYKTAEPVAREIAKRQAFKGVRWMKMTDPAAGEAPSGVGSFLIWQQPHIIYLAELIYR
                     SHPSPEILEKYGKLVDETAAFMYSFADYEKEKDRFVLKGIIPAQETLRASETVNPPFE
                     LSYWYFAMNVAQKWRERMGEKRKVEWDELIAKLSPLASNADGLYLAAETAPETYNDIR
                     FTSDHMAVLGALGILPTNRLINTGYMKNTLHWIWDNWNWDKTWGWDYPMTAMNAARLG
                     EPEKAVGALLMDKRTNTYLVNGHNYQDARLRVYLPGNGGLLTAVAIMCAGWDGSKGKN
                     PGFPKDGRWNVKWEGLKKMP"
     gene            complement(5585..>7099)
                     /locus_tag="PKH58_10655"
     CDS             complement(5585..>7099)
                     /locus_tag="PKH58_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89534.1"
                     /translation="GTGSITEPYATIMKGQTVAVGGDTVYIRGGSYTMTNSNISAYVT
                     LGPYAVVNNLNKSGSSASKRIYYWAYPGEKPVFDFSDVKLSGYRVTAFFVTGSYIHLK
                     GLEVTGMQVTITTHTQSECFRNQGSYNIYENLCMHDGMGIGFYLQSGSNNLVLNCDAY
                     RNWDNVSETKKGSNVDGFGFHPSKGSTGNVIRGCRAWFNSDDGYDCINAYETVVFDNC
                     QAFYNGYNSSFTSLGDGNGFKAGGYGQAPVVSKLPSPVPSHLVRFCVAYRNKSNGFYA
                     NHHVEKEILFYNNTAFRNATNYNMLSQKITKSSFTGNDTTLDCPGIRHKLRNNVSYKY
                     SSYRDTLNIGTSDMLYNSFTPGSGITVNGNDFISLDEALLVAPRLADGSLPDTDFLRL
                     QPSSDLIDKGQNIGFAYSGAAPDLGAFEYQQQTPVDVLPVKSLLFFPDPVSDKLNFTG
                     SSDKNIEIFNCEGKRMLNLKVTYSVDVSNLPAGIYIIRIKADDEKPVISKFIKR"
BASE COUNT         1901 a         1671 c         1400 g         2127 t
ORIGIN      
        1 atagcctcgg ttacatataa aaatcagttt aggtaataca acggataatc attttgaatt
       61 aatacggcaa taaaaaacaa ctaaaaagtt ttctttttta tattcaaatt tacttggttt
      121 tttaacgaaa acaactattt tccggttcgg aatggcatca cgggcaatcc ttttttatta
      181 cacagatttg gcatagcccg ttcgttccac gcaaagcgta ccatctcagg ttgctttact
      241 tcgtcagccc aaacaactac cgtttttctt ttgatttctg ctttcgctgg cacaaaattt
      301 ccatcggcac cggcaatggt aaaccaggtc aaagcctcgc cgtctttact aattaatcca
      361 cctccaatat tatgaaatat aagtatgatt tttccatcct ttatcttcat cgatttataa
      421 cccggtgact gataaataac ttttttattg taatctttcg ctaacgccca caatgaaagt
      481 cgacgggcaa cttcccattt gtacgatgga tgtatatccg acaggttatc caccaaatcg
      541 gtcgtcacca ccattccggt gcggtgtgtg gatagaaaac tgctctgagc ttcccaaaat
      601 tcgggcagca gttcgggtgt gtgctgcatt ttgtcgttgg tgcgctttga atataaataa
      661 ggtgcaatct gaacgtagta aaaaggcatt tgggggtttc ggaagagtga acggtaagtt
      721 tcgagcagca ttttcatctt ttcgggataa gttgcctgat cttctgacat acaattggtt
      781 tcgccctgat accacagcat tcctttaact gcaaaaggta cgatgggttg tatcatactg
      841 taaaattttg tgccggctat ccgtccgttt atttttttat cgttcgggtc tatctgacca
      901 gcaaaaaccg gcgatttttc gtacacccac gcaggtgtcc attcctcaat catggttccg
      961 ccccatgacg atgaaattat ccctaccggc acattcgttt cctcataaat atactttccg
     1021 aaaaaatagc caatggcaga aacattgcgc aaaacactat cgtttgacgt aaaccagcct
     1081 accgatttta tgtcctttgt atcgttttgc ttttcggcat agaggtagcg gattttatct
     1141 gatttgggtt tcaacagttc ctccaaagcc acattttcac cccggacggg agttgcatac
     1201 ttcttcagcc tgtaatcgaa aggatattcc atattcgact gccctgaaca gagccacaca
     1261 tcgcccacca gaatgttttc cagcgttatt gtgttttttc cagatataat catatcagca
     1321 ggttgaacgt tcaatttcaa cgatttaagt gttacactcc attttccatc tgcatccgca
     1381 actgtagtaa cttcttgtcc tgcaaaactc actgttacct tttctccggc ttccgctttt
     1441 ccccaaacgg gtactttagc atcgcgctgt aacaccatgc ccgacgaaat tactttcgga
     1501 agtgtcacat ctgcgtttgc attgaaataa aagaacggga acaataaaaa aatgatgatt
     1561 atgtgtcttg agttcatggc tatagtggtt taattttgat cgtaactgtt gtaaagcggg
     1621 gtaaacggat tttcgtttcc aatgctccgg ataaggatgc agggatggtt tttatcacgt
     1681 tcaacggtgg ttgaaactgg caataaaaat aaaataaaca gcccgaagtt tttcagattg
     1741 cgaaaagtgt gcttcatgtt ggtaaaataa ggtaatctta cgttgtgggc taaagaattt
     1801 gctttagccc acaacggact attcaactcc acaaaaataa tccttttcga aaaaagagag
     1861 gttacaaaaa tgatttatag actgctttat ttgataaaaa cagaaattgc ggcaatcgcc
     1921 gtgatttagt ttttcttttt caacggatat gtttccatca gtttcccgtt tactgtcaaa
     1981 ttgtcgaatt ttacattttc aatcttcccg gtaagtttgt tccctgattt cacaccgtta
     2041 aagttgcagt ttcggacgaa aatatcacgc acacaggtcg tatcgggcag tgcatcgata
     2101 ctaaccccat actggctttt ctgtgaacgg atgtattcca aatttatatt ttgtactaca
     2161 ggatgaaagc ctctttctcc tatctcacgg ggttcataca gcaaactaat atgcagaatt
     2221 gcttccttgc acacacctac ctcaatgtta cggacataaa tattttggat tgttccgcca
     2281 cgtaatgtat tggttttaat ccggataaca cggtcaagca atgggctgtt cattctacag
     2341 ttttcaacga acaggttttt gtatccgcct gaaatttcgc ttccaatcac aactccgccg
     2401 tgcccgtttt tcatttcaca atttctgaca ataatatttt cgcttggttg cgaccacaaa
     2461 cgaccatcgt tgttgcgacc agatttaatc gcgatacaat catcaccggt actgaaatag
     2521 cagttttcga tcagaacatt ggagcaagct tcaggatcgc agccatcgcc gttcggtccg
     2581 tcgttttcga ctcgtactcc tctcacaatc acattattgc acatcagagg atgaataacc
     2641 caaaacggcg atctcaggat tttcactcct tcaatcagca cattgttgca caaatataaa
     2701 ttaatcatct gaggtctcat accgtcattc tcattcatta tacgcatcga aacggggact
     2761 ttgtcgttat tccactgcat cagtttcgga cggccagttc tttgtgatat atccccctct
     2821 ttccagccaa aagaagcagc gccacatttt ttccaccagt tttcattaga cgatgctcca
     2881 tccagcgttc ccttgccggt aatggcaatg ttggtttcgc cgtaggcata gataagcggc
     2941 tgaagattat agcaatccat gccttcccag cgggtaagca ctaccgggaa atacttcgga
     3001 ttggttgtga aaagtaaggt cgcgccttcc tgaacgtgaa gattaacgtt acttttcagt
     3061 gtcagcggag ctgtaaacca tactccggca ggcacaatca ccattcctcc gccacgcagg
     3121 ctgcatgtta caatagcccg gttgatggct tctgtattga gcacggtggt atcgttggct
     3181 acggctccaa aatcgcggat atcaaactga gctttacgaa acaccggagc ttttatattc
     3241 ttttgaatag cagggtatat ctttttccag gcaacagcct gattctgagc attaacaagg
     3301 actactgaaa gcaacagtaa tacaaaaata atatttcttc tcatcgtgtt atcgtattaa
     3361 attgtctatt tttttttatt ctccgggaaa tttttgaatt gcaaattttg aaaaaacacc
     3421 tgaaaaatct gccaaatcag gctttttacg gcattttttt caatccttcc catttcacat
     3481 tccagcgacc gtctttcgga aaaccgggat ttttaccttt actgccatcc catccggcgc
     3541 acataatggc tacagctgtc agcaatccgc catttccggg cagatacacc cgcaaccgcg
     3601 catcctggta attatgaccg ttgactaaat aagtatttgt tcgtttatcc atcagcagcg
     3661 caccgacagc cttttcgggt tcgcccagtc gtgctgcgtt catggctgtc atcggatagt
     3721 cccaacccca ggttttatcc cagttccagt tgtcccaaat ccagtgaagt gtatttttca
     3781 tgtatccggt atttatcagc ctgtttgtcg gtaaaatccc gagagcacca agtacagcca
     3841 tatggtcgga tgtaaaacgg atgtcgttgt aagtttcagg agcagtttcg gccgccagat
     3901 acaggccatc ggcattactt gccagcggcg aaagtttggc tatcagttca tcccactcaa
     3961 ccttacgctt ttcacccatt cgttccctcc atttctgcgc cacattcatg gcaaaatacc
     4021 agtatgaaag ttcaaacggt ggatttacag tttcggaggc acgcagtgtt tcctgagccg
     4081 gaattatgcc tttcaacaca aaacggtctt tttccttttc ataatcagca aaagaataca
     4141 taaacgcggc cgtttcgtcc accagtttgc cgtatttttc cagaatttcc ggtgacggat
     4201 gactgcgata aatcaattct gccaggtaaa tgatgtgtgg ctgctgccag ataaggaaac
     4261 ttcctacacc ggaaggagct tcaccagcag ccgggtcggt cattttcatc caccggacac
     4321 ctttaaatgc ctgtcttttc gcaatttcgc gggctacagg ttcggctgtt ttgtaccagc
     4381 cgagtgtttt ttcgagcatc tccggcctgt tccacaacgc aaactgagcc tgatgccacc
     4441 agatcatttc gagatgaaat tttccgtacc aggagttata cgtcagcccg gtttcctgag
     4501 gcggtgtagt gccggaacat tgtacggcca gcagatactg actcaacacc actcttcgct
     4561 ccagttcttt agctctcgga tcggtacatt ccgaaaaatc cactgctgca ccatctttcc
     4621 aaaaatggtt ccaaaacttt gaggcagcag caaatgtttg ttcagacccc gtattgaaat
     4681 tttcaggctg aacggtagta aaccggcagg taaaactcag ttcattgcca tccgactgaa
     4741 gaacaaaata attcttcgat ttttcaacga gacgggcttt attttcccac gttatgttca
     4801 caaaataaac cgtactgtcc agttgccgtt ttatcagtgc cgaattttct gttttccgaa
     4861 caataacaga actatgttta tcagctgcat tccagttaca ggcatcgtcg gtgtgcgccc
     4921 cggtggggta tgggaattga aattttatgg caggtttttc gccccgggta ctcactcttg
     4981 cagttatctg atccgctgca gggtgtgtag ccgtaagaac ctcatacatg gtatttttca
     5041 cctcaaaccg gctcctgatt gctccgttcc ataaatccag tgtctgtact ggatttttta
     5101 tatcagccag cgcagcacct tccagctcaa gtcccactat tcccagatgc agcctgtgcg
     5161 gattagccct gaaataatca gccgcttcct gctgccgtcc cttttcatta aactggacag
     5221 catatggttc ggctttccct ctgccaaatt caaaattctt cagtgtctct tctggcttgt
     5281 aagcttttac gttggggaaa ctgtgccatc cccattgact ttgagtgccc agaggcattc
     5341 ccttgctata atctttctga aaagtttgca gacccgtaat atcgacggta caggcaaatt
     5401 cgccattccc aacagtaagt gcaccaagcg tatcgacagc ggtaagccgt ggattatttc
     5461 tcgaaacaag tttgtatctg tcaataccag atttaccact taccccgcag gagatgaaaa
     5521 aaattattac aaataaaaag agttttgtct tcattcttca aatgatttaa aattgttctg
     5581 gtactcaacg tttaataaat ttcgaaatta ccggtttctc atcatcagct ttaatcctga
     5641 taatataaat acccgcgggc aaattcgata cgtcaacaga atatgttact ttcagattaa
     5701 gcattctttt tccttcacaa ttgaagattt cgatattctt atccgaactg ccggtaaaat
     5761 tcagtttatc gctgacagga tcgggaaaaa agagcaggga ttttacaggt aaaacgtcca
     5821 caggagtttg ttgctgatac tcaaatgctc ccaaatcggg cgctgcaccc gaataagcga
     5881 aacctatgtt ctgccctttg tcaatcaggt cgctcgaagg ctgtaaacga agaaaatccg
     5941 tatcgggcaa actgccatca gccaacctgg gagctacaag caatgcttca tcgaggctga
     6001 taaaatcatt accattaacg gtaattccgg agccgggagt aaacgaattg tacaacatat
     6061 ccgaagtgcc gatattcagc gtatcgcgat aggaagagta tttgtacgaa acattgttgc
     6121 gtagtttatg ccttataccc gggcaatcga gggtagtatc attgcctgta aaggaacttt
     6181 tggttatttt ctgactcagc atgttgtaat tggtcgcatt cctgaaagcg gtgttgttat
     6241 agaacaatat ctccttctcg acgtgatgat tggcataaaa gccgttggat ttatttcggt
     6301 aagccacaca aaatctgacc agatgagacg ggactggact cggaagcttc gaaacaacag
     6361 gagcctgtcc gtaacctccg gctttgaagc cattgccatc gcccaggctt gtaaatgacg
     6421 agttatagcc attgtaaaat gcctgacaat tgtcgaaaac aaccgtttcg tacgcgttga
     6481 tgcagtcata accgtcatcg ctgttgaacc aggcacgaca accacgaatt acattgccgg
     6541 tagaaccttt ggaaggatga aagccaaaac catccacatt acttcctttt tttgtttccg
     6601 acacattatc ccagtttcga taggcatcgc aattcaacac caggttgttt gaaccgcttt
     6661 gaaggtaaaa tccaatgccc ataccatcgt gcatacatag attttcgtaa atattatagc
     6721 ttccctggtt tctgaaacat tccgattgcg tgtgagtggt gatggttacc tgcattccag
     6781 tcacttccag ccctttcaga tgaatgtatg aaccggtaac aaaaaatgct gtaacacgat
     6841 aacctgaaag ttttacatcc gaaaaatcaa ataccggctt ttcacctgga tatgcccagt
     6901 aataaattct ttttgaagca gaggaaccgc ttttattcag attattcaca acagcgtacg
     6961 gcccgagagt tacataagca gagatatttg aatttgtcat ggtgtaagat ccgcctctga
     7021 tatatacggt atcgcctccc acggctactg tctgcccttt cataatcgta gcgtatggtt
     7081 ctgttattga accggttcc
//
LOCUS       DAOPYB010000161         6994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_13152_length_6994_cov_10.511745, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000161 DAOPYB010000000
VERSION     DAOPYB010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13152_length_6994_cov_10.511745"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3189
                     /locus_tag="PKH58_10660"
     CDS             <1..3189
                     /locus_tag="PKH58_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006043991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNX89535.1"
                     /translation="NPISIDEVEYEITPVADKQGFKVLVCSTDELPQHTIRKKIDFQL
                     RKYFHNYILVFTLENSEHQLWMVPVKTVEKRDLVVVEYQSADKADFLFSKVSNLSFGL
                     EEETNIVDVTHKVHGAFEVNSERITKDFYTGFRKQHNSFVRFIEGISPESDRQWYASV
                     MLNRLMFCYFIQKKGFLNFDTEYLRNKLAWTKLQQGDDQFFGSFYKGFLTVLFHGGLN
                     SPQHSRDFEIKYGRIPYLNGGLFDKHQLEKDYNEIDIPDKAFEDLFDFFDKWHWHLDT
                     RITATGKDINPDVLGYIFEQYINDRAQMGAYYTKEDITEFIGKNCILPFLMDDVAKTS
                     SGRHFAPNGYVWQMLKNSGDKYIYDAVKKGVDEPLPDYIEAGIDTTQPNLLERRARWN
                     EPAFEKHALPTEIWRETVERRQRCTDLRQKIANGEITHINDFITYNLDIRSFVQDLLA
                     TIPDHKFILHFYKALRRISILDPTCGSGAFLFAALNILEPLYEICIERMQEFNAGNDL
                     LFREQLDEMDHKYRSNIQYFIYKSIILRNLYGVDIMHEATEIARLRLFLKMVAVVDVD
                     RRAENLGLDPLPDIDFNIRCGNTLIGYATQQELDNDLTYGDMFANLEFKEKIEEEMDK
                     TARAYKLFKEIQFTQHEDMDAFKDAKNQLNTRLSSLNNELNKKLFASTVTGNALLYTD
                     WLASHQPFHWLAEFYEIIQGNGGFDVIIGNPPYVVYTELRSSYKLVKYETLTCSNLYA
                     FCCERSFILLNIHGRFGMIVPNSSISADKLNPLQKIITKNKRTWISNYSWRPSKLFEG
                     ADMLLAIILSTKNSSELINSSMYQKWYGDYRSNLFENVKYYDVTKVFKEGSIPKFPNE
                     IYFSIINKQNNISNGKTATNYFANSATIHKFYYFRAVQYWIKILEKAPHYLDNGVQIE
                     TGEMKPVYCHERNNKYALISLFSSSLYFLHYITWASCQVMNSRDFNFLIDLETMDKNT
                     IENLSNFGKKIQDDYQKNSHIVVRSYSKKGRDFVMEKQHFLIKKSKPIIDEIDKVLAK
                     HYGFTEEELDFIINYDIKYRMGSELNVEEEEE"
     gene            3192..3959
                     /locus_tag="PKH58_10665"
     CDS             3192..3959
                     /locus_tag="PKH58_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89536.1"
                     /translation="MDVREHYKIPGKVVFIRGNIFIKNKIQKEILDIGCYYEENEFDK
                     IDKIIDGDFTIEDATETSEDTYYYASGGATAFEEGGGFKSRYHYIDRYDRAIDDIVAL
                     SKMNVEEMNKRLLNKLLFASVYSALEVFLQDICGHYVMKSQKYKEGYLKSKSNLKEEI
                     FSEEVDLKIINAVENTVYHKLDIIQPLFEGTFGINFPKYEFIKDNLQIRHDIVHRNGK
                     SKDQSILHVISNSQLYELIEHVDEFVYSLFEEFQKLN"
     gene            4209..4703
                     /locus_tag="PKH58_10670"
     CDS             4209..4703
                     /locus_tag="PKH58_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89537.1"
                     /translation="MKFKIVKLERFSGNKAGVYSVALNNDEETLLNKFVRENEISFKS
                     ETKDIVKRLISIGHTTGARSQFFKEWEGKPGDGVCALYDKPDSNLRLYCICYGTQLII
                     IGSGGHKPKTIRALQEDKKLTEENALIKEISRQITQRIKDKEIRFTPDYLDFTGDLSF
                     NDED"
     gene            4708..5121
                     /locus_tag="PKH58_10675"
     CDS             4708..5121
                     /locus_tag="PKH58_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX89538.1"
                     /translation="METYNDEALNSLLSEITPEEQAKTDAKMLLAVKIADAMQAKGWN
                     KTRLMKEMGKSNPSEITRWLSGTQNFTVDTLVDLERVLEIKLLDLGEAKSTEKTIRQY
                     KIDIVVPFNRNEKLSACLVSEPDDVYTYYKQTIYS"
     gene            5125..5547
                     /locus_tag="PKH58_10680"
     CDS             5125..5547
                     /locus_tag="PKH58_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89539.1"
                     /translation="MSKETISFKFRNIDILEFSFVHPKQIFPENAVFRFETQIEHKVN
                     LEEKFIIVVSSFIIHCENLSEKVGNAVVSCVYTIDNIEDFVNENKVFTLPEQLLTMFN
                     SISISTCRGVLFTLFRGTPLHTVILPIINPVDLNKQQP"
     gene            5561..6019
                     /locus_tag="PKH58_10685"
     CDS             5561..6019
                     /locus_tag="PKH58_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNX89540.1"
                     /translation="MEIQYKGNIELLQLPKAAFLSSRNISPQSVMACYDWATQQRSAG
                     RCVISGFHSQIEKDVLHFLLKGTQPVILVLGRTLYKNLPDELIKPLEEGRLLIVSVVS
                     TPRQSEETAQRRNRYILDNADEVVFGSLDVTGNLYPLYKEIKENKKYIIL"
     gene            6024..6659
                     /locus_tag="PKH58_10690"
     CDS             6024..6659
                     /locus_tag="PKH58_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89541.1"
                     /translation="MTKLEKLYQSIANFKELGLALNQEMLQKADNLEEELIKNEVIPR
                     LTGSIEPIITQIQRPIVLVVDYVPGEPLSVRLTRKRVITDDAATKEYTLAPKAKEPEK
                     KSHKTITSAPKAPWTGLVVTYPDGKVINNKYAYETLIQVVEKAGVEKVAKLGMKQVGL
                     EFISKTRDDFYNQHELPGGYLITTHSSTAKKKQQIEEISKKLGLGLKVQVV"
BASE COUNT         2447 a         1256 c         1382 g         1909 t
ORIGIN      
        1 aatccaatca gtatcgacga agtagaatac gaaattactc ccgtggccga caagcaggga
       61 tttaaagtcc tggtttgttc cactgatgag cttccgcagc ataccatccg gaaaaaaata
      121 gactttcagc tccgtaaata ttttcacaac tacattcttg tttttacgct cgaaaattcg
      181 gagcatcagc tctggatggt tcctgtaaaa acggtggaga aaagagatct tgtagtggtg
      241 gaatatcaga gtgccgacaa agcggatttt cttttcagca aagtatctaa tttaagtttt
      301 ggcctggaag aagaaaccaa cattgtagat gtaacgcaca aagtacacgg agcttttgaa
      361 gtaaattcgg agcgtataac caaagatttc tatacgggtt tccgcaaaca gcacaattcg
      421 tttgtcaggt ttatagaggg aataagcccc gaaagcgacc gacaatggta tgcttccgta
      481 atgctcaacc gcctgatgtt ttgttatttt atccagaaaa aaggatttct gaatttcgat
      541 accgaatacc tgcgtaataa gctggcatgg acaaaactac agcagggtga tgaccaattt
      601 ttcggatctt tttacaaagg gtttctcaca gttttgtttc acgggggact gaattcacca
      661 caacacagtc gagattttga aatcaaatac ggacgcattc catacctgaa cggtggactg
      721 tttgataaac atcagctgga aaaagattac aacgagatag atattcccga taaagcattc
      781 gaagatctgt tcgatttctt tgacaaatgg cactggcatc tcgacacacg cattacggct
      841 accggaaaag acatcaaccc cgatgtgctg ggctatattt tcgagcaata catcaacgac
      901 cgcgcacaga tgggtgctta ctacaccaag gaagatatta cggaatttat tggtaaaaac
      961 tgtattctgc cattcctgat ggatgatgtt gcaaaaactt cgtccggaag gcattttgca
     1021 cccaacgggt acgtgtggca gatgctgaaa aacagcggcg ataaatacat ttacgatgcc
     1081 gtgaaaaaag gagtggacga gcccttgccc gattatattg aagccggcat tgataccacg
     1141 caacccaacc tgctggaacg ccgtgcccgc tggaatgaac cggcctttga aaaacatgct
     1201 ttgcctaccg agatatggcg cgaaacggta gaacgccggc aacgctgtac cgatctccgg
     1261 cagaaaattg ccaacggaga aatcacccac atcaacgatt ttattaccta caacctggat
     1321 atccgctcgt ttgtgcagga tttgcttgcc acgatacccg accataagtt tattctgcac
     1381 ttctacaaag cactgcgccg tatttccatt ctcgacccta cctgcggttc gggagctttt
     1441 ttgtttgcag cgctgaatat tctggaaccg ctttacgaaa tatgtataga acgtatgcag
     1501 gagttcaacg ccgggaatga tctgctgttc cgggaacaac tggacgaaat ggaccacaaa
     1561 taccggagca atatccagta ttttatctac aaaagcatta tattgcgaaa cctctacggt
     1621 gtggacatta tgcacgaagc taccgaaata gcccgcctgc gcctgttcct gaaaatggtg
     1681 gctgtggtag atgtggatcg ccgtgccgaa aacctgggac tggacccgct gcccgatatc
     1741 gacttcaaca tacggtgcgg aaatacgctg ataggatatg ccacccaaca ggaactggac
     1801 aatgatctta cctacggtga tatgtttgcc aacctggagt ttaaggaaaa gatagaggaa
     1861 gaaatggata aaactgccag agcttataaa ctattcaaag aaatccagtt tacacagcac
     1921 gaagatatgg atgcttttaa ggacgcgaaa aaccaactga atacccgtct cagcagcttg
     1981 aataatgaac tgaataaaaa attatttgct tcaactgtaa cgggtaatgc cctgttatat
     2041 accgattggc ttgcctcgca ccaacctttc cactggttgg ctgaatttta tgaaattata
     2101 cagggtaatg gtggatttga tgtgattatt ggaaatccgc cgtatgttgt ttacactgaa
     2161 ttaagatcat catataaatt ggtaaaatac gaaacactta catgttcaaa cttatatgca
     2221 ttttgttgtg aaagatcttt tattctcctt aatattcatg gaagatttgg aatgatagtt
     2281 ccaaatagca gtatttcagc agataagtta aatcctttac aaaaaataat aaccaaaaat
     2341 aaaagaactt ggattagtaa ttattcatgg agaccatcaa aattatttga aggtgcagat
     2401 atgttactag ctattatttt gtcaacaaaa aatagttctg aattaataaa ttcctcaatg
     2461 tatcaaaaat ggtatggtga ttatagatca aatttatttg aaaatgtaaa atattatgat
     2521 gtcacaaagg tttttaaaga aggtagtata cccaaatttc caaatgaaat ttacttttca
     2581 ataattaaca aacaaaataa tatttcaaat ggcaaaacag caacaaacta ttttgcaaat
     2641 tcagcaacaa tccataagtt ctattacttt agagctgtac aatactggat taaaatttta
     2701 gaaaaagccc cacattatct agataacggt gttcaaattg aaacaggaga gatgaaacct
     2761 gtttattgtc atgaaagaaa taataaatat gccttaatat cacttttttc ttcttcatta
     2821 tattttttac attacataac atgggctagt tgccaagtta tgaatagtag ggattttaat
     2881 tttctaattg atttagagac aatggataaa aatacaatag aaaatttatc aaattttgga
     2941 aagaaaatac aagatgatta tcagaaaaat agccatatcg tagttcgtag ttattctaaa
     3001 aaaggtagag actttgttat ggaaaaacaa catttcttaa ttaaaaaatc caaacccatc
     3061 attgacgaaa tcgacaaagt gttagccaaa cattacggtt ttacggaaga ggagttggat
     3121 tttattatca actacgatat caagtataga atggggagtg agctgaatgt ggaggaggaa
     3181 gaagaatgag tatggatgta agggaacact ataaaatacc cggtaaagta gttttcatca
     3241 gaggcaatat ctttatcaaa aataaaattc aaaaagagat tttggatatt ggatgttact
     3301 acgaggaaaa tgaatttgat aaaatcgata aaattataga tggtgatttt acaatcgaag
     3361 atgctacaga aacctccgaa gatacctatt attatgcttc aggcggagca actgcttttg
     3421 aagaaggtgg aggatttaag agccgttatc actatatcga caggtacgac agagcaattg
     3481 atgatatagt tgctttatca aaaatgaatg ttgaggaaat gaataaacgc ttgttgaata
     3541 aacttttatt tgcaagtgtc tattctgctt tggaagtatt tctgcaggat atatgtggtc
     3601 attatgtaat gaaaagtcaa aaatacaaag aggggtatct caaatcaaaa tcgaatttga
     3661 aagaagagat attttcagaa gaagttgacc tgaagataat aaatgctgtt gaaaacacag
     3721 tatatcataa attagatatt atacaaccgt tatttgaagg aacttttgga attaattttc
     3781 caaaatatga atttattaaa gacaatttgc aaatacgaca cgatatcgtc cacagaaatg
     3841 ggaaatcaaa agaccaatct attttgcatg taataagcaa tagtcagtta tatgaattaa
     3901 tagaacatgt tgatgaattt gtatattctc tctttgaaga atttcaaaaa ttgaactgat
     3961 atcgagaaaa ttaaaaataa aaaaaaatca cttttaccga aagtttttaa cgtgtggtaa
     4021 aatcccgata aattaccgat aaatcgggac agactctaaa tggagaatag gagcagctaa
     4081 acggattaat aaaatttgta ccgaaaaaga aatgcaggca gtttgcaata ccgaatgaaa
     4141 tgtagaaaat aacaaataaa tttcacttat aagtaaaatt atattatctt tgtaaaccaa
     4201 tttctgaaat gaaatttaaa attgtaaagc ttgaacgatt tagtggaaac aaagcaggtg
     4261 tttattcagt ggctttaaac aacgatgaag aaacgttgct gaataaattt gtgcgcgaaa
     4321 atgaaatttc atttaaaagt gaaacaaaag acatagtaaa acgtttaatt agtataggac
     4381 atacaaccgg agcacgtagt cagtttttca aagaatggga aggtaaaccc ggagatggtg
     4441 tatgtgcctt gtacgataaa cctgatagta atttaagact atactgtatt tgctacggta
     4501 ctcaactcat aattattgga agcggcggac acaaacctaa aacaatacgc gcattgcagg
     4561 aagataagaa actgacagaa gaaaacgcat tgataaagga gatttcccga caaatcacac
     4621 aacgcatcaa agacaaagaa atccggttta cacccgatta cctcgatttt acaggcgact
     4681 tatcattcaa cgacgaagac taaaaacatg gaaacgtata acgacgaagc tttaaatagc
     4741 ttattatcgg aaattacccc cgaagaacag gcaaaaacag atgcaaaaat gctgcttgcc
     4801 gtaaaaatag ccgacgctat gcaagccaaa ggctggaaca aaacgcgact tatgaaggaa
     4861 atgggcaaat caaatccttc ggaaataacc cgttggctca gtggaactca aaacttcacc
     4921 gtagatactt tggtagattt agaacgggtg ttggaaatta aactccttga tttaggtgaa
     4981 gcaaaaagca ctgaaaaaac gatcaggcaa tataagatag atattgtggt gccatttaac
     5041 agaaacgaga aattgtcggc ttgtttagtt agtgagcctg acgatgttta tacctattac
     5101 aaacaaacca tttattcgta gcgtatgtca aaagaaacca tttcgttcaa attcagaaat
     5161 attgatatac ttgaattttc ttttgttcat ccaaaacaaa tatttcccga aaatgctgtt
     5221 tttcgttttg aaacacagat tgagcataaa gttaatttgg aagagaaatt tattattgtt
     5281 gtttcttctt tcatcattca ctgtgagaat ttaagtgaga aggtaggtaa tgcagtggtt
     5341 agctgtgttt acaccataga caacattgag gattttgtga atgaaaataa ggtgtttaca
     5401 ttgcctgaac aattacttac tatgttcaat tccatttcaa tttccacttg tcgtggagta
     5461 ttgtttacac tattcagagg tacgccgttg catacggtta ttttgccaat tatcaatcct
     5521 gtcgatctaa acaagcaaca accctgaaaa tctgatagct atggaaattc aatataaagg
     5581 taatattgaa ttgcttcaac ttcccaaagc agctttctta agcagccgaa atatatcgcc
     5641 gcaatcggta atggcctgtt acgactgggc tacgcaacaa cgtagtgccg gtcgttgtgt
     5701 tatcagtggg tttcattctc agattgaaaa agatgtactc cattttttac tgaaaggaac
     5761 acagccggtc atactggtgt tgggtcgcac actgtataaa aacctgcctg atgaattgat
     5821 taaaccactt gaagaaggaa gattactgat cgtttctgtc gtatcaactc cccgtcagtc
     5881 ggaagaaaca gcacaacgcc gaaaccggta catcctggat aatgccgatg aagtggtttt
     5941 tggttcgttg gatgtaacag gtaatttata cccactctat aaggaaataa aggagaataa
     6001 aaaatacatt atattgtaat accatgacca aactcgaaaa attgtatcaa tccattgcca
     6061 actttaagga attaggtttg gcgttgaacc aggaaatgct tcagaaagcc gataatctgg
     6121 aagaagaact cattaagaat gaagtcattc cacgccttac cggcagcatc gaacccatca
     6181 tcacccagat acagcgtccc attgtgctgg tagtcgatta tgtaccgggc gaaccgctca
     6241 gcgtgcgcct cacccgtaag cgcgtaatca ccgatgatgc tgctactaaa gaatatacac
     6301 tggcacccaa agcaaaagag cctgaaaaga aatcgcacaa aacaatcacc tcagcaccta
     6361 aagctccctg gaccggactg gtggttacct atcccgatgg taaagtgatc aataacaaat
     6421 atgcctacga aacacttatt caggttgtgg aaaaagcggg cgttgagaaa gtggcaaaac
     6481 tgggaatgaa acaggtgggg ctggaattca tttccaaaac ccgcgatgat ttctacaacc
     6541 agcacgaact tccgggcggc tatctgatta ccacccactc atcaactgca aaaaagaaac
     6601 agcagataga agaaatttcg aagaaactgg ggctggggct gaaggtacag gtggtgtgag
     6661 aaatacagac atcatccgaa acggagggaa aaaaaagatc tgcaaactaa aacaaaaaat
     6721 taattaaacc taacaatctc ctctcactta aatgtcccct tgtaagggga cgagcgtagc
     6781 ggaggggtta tttaagcact ctgagcacag cggagggatt atataaacat cctgagcgca
     6841 gcgaaggggt aaaagtaatt tttattcctg tttatcattt aatcacataa atcaaagttc
     6901 agacagttgt cagttcaact tatcttataa atcaataatc aaacaattat atgcccgaaa
     6961 cagaaaaaat aaataccgat agctctgatt atct
//
LOCUS       DAOPYB010000162         6923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_13342_length_6923_cov_8.481800, whole genome shotgun sequence.
ACCESSION   DAOPYB010000162 DAOPYB010000000
VERSION     DAOPYB010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13342_length_6923_cov_8.481800"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PKH58_10695"
     CDS             complement(<1..901)
                     /locus_tag="PKH58_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144814.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNX89542.1"
                     /translation="MVRYLNYNTVLKSEFTERVQKISINAGFTCPNRDGSKGTGGCTY
                     CNNQTFSPDYCKSAKSVSQQIEEGIAFFNHKYEAQKYLAYFQSYTNTYDKLENLTQIY
                     EEALINPKVVGIVIGTRPDCVSDELLDYFAELNKQYYVMIEYGVESTSDDTLKLINRG
                     HDYACAEQVIRKTAARGLRTAAHLILGLPGESRETLLTHAKKISGLPLTAIKLHQLQL
                     VRGTVMARQFDEHPERFNMMSAMEYIDLVIDFLEILHPQIAVERFISQSPPRLLIAPD
                     WGLKNFEFVSKIEKRLVERDTWQG"
     gene            complement(1134..2876)
                     /locus_tag="PKH58_10700"
     CDS             complement(1134..2876)
                     /locus_tag="PKH58_10700"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNX89543.1"
                     /translation="MDELLKEVTARAQVWLDGNYDAETKAEVKKLMEAEDKTDLIEAF
                     YRDLEFGTGGLRGIMGAGSNRMNIYTVGAATQGLSNYLKSQFAALPQISVVIGHDCRN
                     NSRKFAEISANIFSANGIKVYLFEDLRPTPEASFAIRHLGCQSGIILTASHNPKEYNG
                     YKAYWNDGAQMIAPHDENVITEVLKIKNVDDIKFEGNPGLIEIIGEEIDNVYIDKIKT
                     LILSPEAIERNKDLKIVYTPIHGTGIKLIPKTLREYGFTNIIDVPEQNVISGDFPTVV
                     SPNPEEPAALAMAVKKAIETDADIVMASDPDADRIGIAIKNDKGEWILVNGNQTFLIF
                     IYYLITKWKELGRLTGNEYVVKTIVTTEVMADIAIKNNVEYYNCFTGFKWIADVMRQN
                     EGKKTYIGGGEESYGFLAEDFVRDKDSVSACALIAEITAWAKDNGKTLFEMLQDIYIE
                     YGYGKEKGISVVRKGKSGAEEIAQMMADYRNNPPKEIAGSVVKTIKDYQTLKMTDVKS
                     GEVSDLDMPATSNVLQFFTEDGTKISVRPSGTEPKIKFYIEVRGEMKTRADYDAAEAA
                     ANLKVEAVRASMGV"
     gene            complement(3014..3358)
                     /locus_tag="PKH58_10705"
     CDS             complement(3014..3358)
                     /locus_tag="PKH58_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX89544.1"
                     /translation="MKFDRFEEIIAWQKSKDLCKEIYLLFENSKDYSFKDQIQRATVS
                     IMNNIAEGYERRTNNEFKHFLYIAKGSCGEVRSMLALALELNKINKTDFDKFYKQSEE
                     ISKMLSGLIKTL"
     gene            complement(3496..4875)
                     /locus_tag="PKH58_10710"
     CDS             complement(3496..4875)
                     /locus_tag="PKH58_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX89545.1"
                     /translation="MTEKTIASLRESKTARWAALAVVAFTMLCGYYLTDIMAPLKGLL
                     EGQIGWSSSDFGFFNSAYGWFNVFLGMLIIGGILLDKLGVRITGLGAAIVMVTGTLIK
                     YWAVSTHSLDGQMIIGVKSQVFFAGIGFATFAVGVEVAGITVSKIIYKWFKGKELALA
                     MGLEMATARLGTALALSTSVPIAKALGHIDVSRPILVCLIMLCIGLISFIMYTFMDKK
                     LDASEKTNEAKNPEDEFKMKDIVDIVKNKGFWYIAILCVLFYSAVFPFLKYAADLMVN
                     KFGVEQSLAGAIPALLPFGNILLTPLFGGIYDKKGKGASIMILGSLILIFVHAMFSLP
                     IINNWIIAIVLILLLGIGFSLVPSAMWPSVPKIIPEKQLGTAYALIFWVQNWGLMGVP
                     ALIGWVLDKYCITGQTVREGLTVNTYNYTLPMAIFTCFGVLAVIFAFLLKAEDKKKGY
                     GLEEPNIKK"
     gene            5344..6189
                     /locus_tag="PKH58_10715"
     CDS             5344..6189
                     /locus_tag="PKH58_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX89546.1"
                     /translation="MEQEYTTVIKPKNRLLELNLKEIWQYRDLFSMFVKRNIITQYKQ
                     TILGPAWFVIQPALTTIMYMVVFGGIAGISTDGLPQPLFYLSGIVCWQYFSDCLNKTS
                     STFTDNQSIFGKVYFPRLIIPLATVASNLVRMFIQFALFVVVYLWFVFKGVSVAPNIY
                     LLIIPLLILMLAGLSLGFGIIISSLTTKYRDLTILFTFVVQLWMYATPVIYPLSTISN
                     EKIRLAMMINPITSIVEAFKYSTLGVGTFSWNMLGYSFGFMLVLLAIGIVIFNKVQRS
                     FMDTV"
     gene            6233..6622
                     /locus_tag="PKH58_10720"
     CDS             6233..6622
                     /locus_tag="PKH58_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNX89547.1"
                     /translation="MADILYKEESYKIIGTCMKVHSELGAGFLESVYQEALEKQFIKD
                     GIPYEREKLLKIDFDGQQLKKSFKADYVCYNKIIVELKAAIFIHNDNIEQTRNYLKAT
                     KLQLGLIVNFGETSLKYKRVLNSYNSH"
     gene            6631..>6923
                     /locus_tag="PKH58_10725"
     CDS             6631..>6923
                     /locus_tag="PKH58_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX89548.1"
                     /translation="MRMISIEFNNISKQYRLGLVSTRTLSHDLNRWWKMNMLRQDDPY
                     LKIGEVNDRAHRGESEYVWALRDIDFKVEQGDVLGIIGKNGAGKSTLLKILSKV"
BASE COUNT         1994 a         1500 c         1337 g         2092 t
ORIGIN      
        1 ttccctgcca ggtatctctt tcgaccagtc ttttttcaat tttacttacg aattcaaaat
       61 ttttcaatcc ccagtcagga gcaatcaata acctgggtgg agattgtgat ataaagcgct
      121 caaccgcaat ctgaggatga agtatttcca gaaaatcaat caccaggtca atgtattcca
      181 ttgccgacat catgttgaac ctttcgggat gttcgtcgaa ttgtcgggcc atgacagtac
      241 ctctcaccaa ttggagttga tgcagcttaa tggcggtaag cggtaacccg gatatttttt
      301 tcgcatgtgt tagaagcgtt tccctgcttt cacccggcag tcccagtatc agatgtgctg
      361 ctgtacgcaa tcctctcgca gctgttttcc ggataacttg ttctgcacag gcatagtcat
      421 gaccccgatt gattaatttc agtgtgtcgt cactggtact ttccacgccg tattctatca
      481 tcacataata ctgtttgtta agttcggcaa aataatccag caattcatcg ctcacacaat
      541 caggccgggt gccgataact atcccaacca ctttcggatt gatgagtgct tcttcgtaaa
      601 tctgtgtcag attttcaagt ttatcgtagg tgttggtgta gctttggaaa taagccaggt
      661 atttttgagc ttcgtactta tggttgaaga aggcgatacc ttcttcgatt tgctgtgaaa
      721 cagatttagc agatttgcaa taatccggac tgaatgtctg attgttgcaa taggtacaac
      781 cgcctgttcc tttgctgcca tcgcggttgg ggcaggtgaa gccggcatta atcgatattt
      841 tctgaacacg ttcagtaaac tctgatttca gaacagtatt ataattgaga taacggacca
      901 tatttgccca ctaaccccct gaagggggaa ttaagtacag tttattctat tttgtgaatg
      961 gtgatgcaac agagatatag atcagattgc atactcctta aaaaaacttt aatgggtagt
     1021 aaaaattgcg tattcaggta aagaccagaa tacgcaattc aaaaaaatat tttatataaa
     1081 aagtaagcct gataaatgca tttaagtcct cccctttagg gggccagggg gtattacact
     1141 cccatcgatg ctcgtacggc ttccactttc aggttggcag cagcttcggc agcatcgtaa
     1201 tctgcacgtg ttttcatttc accacgtacc tcgatataga actttatttt aggttctgtt
     1261 cccgacggac gaaccgaaat tttcgttcca tcttcggtaa agaactgcaa tacattggaa
     1321 gtggcaggca tatcgagatc gctaacttca cccgatttga catctgtcat tttcagtgtt
     1381 tgataatctt tgatcgtttt tacaaccgaa ccggcaattt ctttcggagg attgttgcga
     1441 taatcagcca tcatctgtgc aatttcttcg gctcctgatt tgcctttccg gaccaccgaa
     1501 atgccttttt cttttccgta accatattca atatagatgt cctgaagcat ttcgaacaag
     1561 gttttaccat tatccttagc ccatgcggtg atttcagcta tcagtgcaca tgccgataca
     1621 gagtctttat cgcgaacgaa gtcttcagcc agaaagccgt aactttcctc tcctccgcca
     1681 atatacgttt ttttaccttc attctgtcgc atgacgtcag ctatccactt gaaaccggtg
     1741 aagcaattgt aatattccac attgtttttg attgcaatat ctgccatcac ttcagtggta
     1801 acaatcgttt ttaccacata ctcattgccg gtaagtctgc caagctcttt ccatttggta
     1861 atcaggtagt aaatgaaaat gaggaatgtc tggtttccgt ttaccaaaat ccattcgccc
     1921 ttatcgtttt tgatggcaat gccaatgcgg tcagcatcag ggtctgaagc cattacgata
     1981 tcagcatcgg tttctatggc tttttttact gccatcgcca gtgcagccgg ttcttcggga
     2041 tttggagata ctacggttgg aaagtcgcca ctgataacat tttgttcagg gacatcaatg
     2101 atgtttgtaa aaccgtattc acgcagggtt tttggaatca atttaatgcc ggttccgtga
     2161 atgggagtat atacaatctt gagatccttg ttcctttcga tagcttcagg agacagaata
     2221 agtgttttaa ttttgtcaat gtacacgttg tcaatttctt ctccgataat ctcgatcagt
     2281 ccgggattac cttcaaattt aatgtcatcc acatttttga ttttcagaac ctccgtgata
     2341 acattttcat cgtgcggagc aatcatctgc gcaccatcat tccagtaagc cttgtagccg
     2401 ttgtattctt tcggattgtg cgaagcagtg agaataattc cgctctggca acctaaatga
     2461 cgaatagcaa aggatgcttc cggggtaggt ctgaggtctt cgaaaagata gacttttata
     2521 ccgtttgccg agaatatatt ggcgctgatt tcagcaaact tccggctgtt gttacggcag
     2581 tcgtgaccta tcaccacgct tatttggggc agtgccgcga actgtgattt caggtaattg
     2641 ctcaaaccct gagtggctgc gcccaccgta tagatattca tccggtttga accagcgccc
     2701 ataatgccgc gaagtcctcc tgttccaaat tccaaatccc ggtagaaagc ttcgataaga
     2761 tcggttttat cttcagcttc catcagtttt ttcacttcag ctttcgtctc agcatcgtag
     2821 ttgccatcga gccacacctg tgcccgggcg gttacttctt ttaatagttc atccatagtt
     2881 tgtcaagttt atcaagtaga aagtttatca agtaagagag ttcatcaagt ttgtcaagtt
     2941 tatcaagtaa cagagtttat caagtttgtc aagtatataa agtaaaaaaa agagaacttt
     3001 acgaactttt cacttaaagt gttttaatta atcccgagag cattttagat atttcttcgg
     3061 attgtttgta aaatttatca aagtctgttt tattaatttt attcaattct agagccaaag
     3121 ccagcatcga acgtacttca ccacaagaac cttttgcaat atacaagaaa tgtttgaatt
     3181 cattattagt acgccgttca tatccttctg ctatattgtt cataatcgaa actgtcgctc
     3241 gttgaatttg atctttgaag gaatagtctt tcgaattctc aaaaagtaaa tatatctctt
     3301 tgcacaaatc ctttgatttt tgccatgcaa taatttcttc aaatctatca aacttcatta
     3361 ctttgttttt atatgaaatt cttcaaatta aaaaatttac cgttatgaaa taaactttat
     3421 gaacttgaag aactttcaga ctttacagac tttacaaact ttacaaactt tataaacttg
     3481 atgaacttta taaactcatt tttttatatt cggttcttcc agtccgtagc cttttttctt
     3541 gtcttcggct ttgagaagga atgcaaagat aacggcaagc actccgaagc aggtgaaaat
     3601 agccattggc agcgtataat tgtatgtgtt tactgttaat ccttcgcgta cagtctgacc
     3661 ggtgatgcaa tatttgtcaa gcacccagcc gatgagtgca ggaacaccca tcaatcccca
     3721 gttttgtacc cagaaaataa gcgcgtatgc tgttcccaac tgtttttcag gaataatctt
     3781 cggaacagat ggccacatag ccgaaggaac caacgagaaa ccgataccca gcaataaaat
     3841 taaaacaatg gcaattatcc aattgtttat gatcgggagg gagaacatgg catgaacgaa
     3901 aatcagtatt aatgatccta aaatcataat ggaagctcct ttacctttct tgtcgtaaat
     3961 accaccgaat agtggagtta agagaatatt tccgaaaggc agcagggcag gaatggctcc
     4021 ggcaagcgac tgttcaacac cgaacttgtt gaccatcagg tcggcagcat atttcaggaa
     4081 tgggaatacg gctgaataaa acagtacgca cagaatggcg atataccaga aacctttatt
     4141 ttttacgata tcaacgatat ctttcatttt aaattcgtct tccggatttt tcgcctcatt
     4201 ggttttttcc gaagcatcca gctttttatc catgaaagtg tacatgatga acgatatcag
     4261 cccgatacac agcatgataa gacaaaccag aatagggcgc gatacatcaa tatgtccaag
     4321 cgctttggca atcggaaccg aggtggaaag tgccagagca gtacccagac gggcagtggc
     4381 catctccaga cccattgcca gggcaagttc ttttcctttg aaccatttgt aaattatttt
     4441 tgaaacagtg atgcctgcca cttcaacacc aacagcaaaa gttgcaaaac cgatacccgc
     4501 aaagaaaacc tgcgatttga cacctattat catctgaccg tcgagtgagt gtgtggatac
     4561 agcccagtat tttatcagcg taccggttac catgacaata gcagcaccaa gtccggtgat
     4621 acgaacacct aatttgtcga gaaggatacc accgatgatt agcataccga ggaatacatt
     4681 aaaccagccg tacgcgctgt tgaaaaatcc gaagtcactg ctcgaccaac cgatctgacc
     4741 ttcaagaagt cctttgagcg gcgccataat gtcggtaaga taatatccgc ataacatggt
     4801 aaatgctact acggcaaggg cagcccagcg ggctgttttc gattcacgaa gtgaggcaat
     4861 tgttttttct gtcatattta tataatttag tttgtcaagt tcatcaagtt tgtaaagtaa
     4921 aaaagttcag gaagaaagaa agttaataac tcataactca taactcataa ttcataactt
     4981 accgttcacc tttccccgtc accttgtctc cttgtcttct catcactctt atccccgtct
     5041 tcgcatacta ttcgtgaaat tcgtatattt cttaattcgt acaattcgca tatcagtata
     5101 aacgaatgca aatctacaga aaaaaatcaa aaaacggata tttgaaccaa aatttcaatt
     5161 aaatagggta aatgctcttc gttgagtatt aacaaaattg aaaatttaat tacatttgca
     5221 attgaaaata atttatcaac ttaataatgt aaaattctat cgtaataaac ttaaagaacc
     5281 ttctatttga taactctttt tttacttgat aaactttaca aacttgatga actgtaacac
     5341 taaatggaac aagaatatac caccgtcata aaacccaaaa accgtctcct ggagctcaat
     5401 ctgaaggaaa tctggcagta ccgcgatttg ttcagcatgt ttgtgaagcg caacatcatc
     5461 acgcagtaca agcaaaccat cctcgggcct gcatggtttg tcattcagcc cgctctgacc
     5521 accatcatgt acatggtagt tttcggaggc attgccggta tcagcaccga cggacttccg
     5581 caaccgctgt tctacctgtc gggaatcgtg tgctggcagt atttctccga ttgtctcaac
     5641 aagacttcat caacttttac cgataatcag agcatttttg gtaaggttta tttcccccgc
     5701 ctcatcattc cgctggctac tgtcgcctcc aacctggtac gcatgtttat tcagtttgct
     5761 ctcttcgtgg tggtctatct ctggtttgtg ttcaagggag tatctgtcgc gcccaatatt
     5821 tacctgctta tcattccgtt gcttatactt atgcttgccg gattatcact gggtttcggc
     5881 atcatcatat cctcactcac caccaaatac cgcgatctca ccatcctctt tacctttgtg
     5941 gtgcagctct ggatgtatgc cactccggtt atttatccgc tgagcaccat tagcaatgag
     6001 aaaatacgcc ttgccatgat gatcaacccc atcacttcca tcgtagaagc cttcaaatac
     6061 tccacactgg gtgtaggcac cttcagctgg aacatgctcg gctatagctt cgggtttatg
     6121 ttagtacttc tggccatcgg catcgtaata tttaataaag tgcagagaag ttttatggac
     6181 accgtttaaa taacacgaat ttcaccaatt aacacgaatt aaacgaatta agatggcaga
     6241 tatactgtat aaggaagaaa gttataaaat aataggaact tgtatgaagg ttcattccga
     6301 actgggagca ggttttctcg aatcagttta tcaggaggca ttggaaaagc aatttataaa
     6361 agatggaatt ccatacgaac gtgaaaaact attgaaaatc gattttgacg gacaacaatt
     6421 aaaaaagtct ttcaaagccg attatgtatg ttacaacaaa ataattgtag aattgaaggc
     6481 tgctattttt attcataatg acaatattga acaaacaaga aattatttaa aggcaacaaa
     6541 attacaactc ggattaatag ttaactttgg cgaaacaagc ctgaaataca aacgtgtact
     6601 aaattcgtat aattcgcatt aattcgtaaa attcgcatga tatcaataga attcaacaac
     6661 atatccaaac aataccgcct gggcctcgtt tctacccgga cactctcaca cgacctcaac
     6721 cgctggtgga agatgaacat gctgcggcag gatgatccgt atctgaagat aggggaggtg
     6781 aacgaccggg cacaccgggg cgagagcgaa tacgtgtggg cgctgcgcga cattgatttt
     6841 aaggtggagc agggcgatgt gctgggcatt ataggtaaga acggcgcggg aaaatccacc
     6901 ctgctgaaaa tcctctccaa agt
//
LOCUS       DAOPYB010000163         6857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_13501_length_6857_cov_9.471920, whole genome shotgun sequence.
ACCESSION   DAOPYB010000163 DAOPYB010000000
VERSION     DAOPYB010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13501_length_6857_cov_9.471920"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..920
                     /locus_tag="PKH58_10730"
     CDS             15..920
                     /locus_tag="PKH58_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNX89549.1"
                     /translation="MKPTLFVLAAGMGSRYGGLKQLDGLGPNGETIMDYSVFDAERAG
                     FGKVVFVIRKSFEEDFRNIVLKKYEGVIETEVVFQDITDVPEGFTYNPERTKPWGTNH
                     AVLMGKDVIREPFAVINADDFYGKDAFAVMAEFLRGVTGKQNEYCMVGYRVGNTLSES
                     GSVSRGVCVVDENGNLTNVVERTSIERKGDDIVFIDEEGKEVVIGENTPVSMNMWGFT
                     PDYFDYSLDDFKKFLTEYGNELKSEFFIPLVVNNLIVSGKVTCKVLDSTAKWFGVTYA
                     EDRAEVVAKIQKLIDEGIYPAKLYK"
     gene            960..1994
                     /gene="galE"
                     /locus_tag="PKH58_10735"
     CDS             960..1994
                     /gene="galE"
                     /locus_tag="PKH58_10735"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNX89550.1"
                     /translation="MTKILVTGGTGYIGSHTVVELQNAGFEVVIVDNLSNSNIEVLNG
                     IEKITGIRPAFENIDCTDYVNMDRMFEKYNGIEAIIHFAASKAVGESVEKPLLYYRNN
                     LLSLINLMQLMPIHNVKNLVFSSSCTVYGQPDILPVTESAPIKPALSPYGNTKQICEE
                     IIRDTAYSNSFYQATILRYFNPIGAHPSAEIGELPNGVPNNLVPFITQTAAGVRKQLQ
                     VFGNDYNTPDGTCIRDYINVVDLAKAHVIAVKRMLEHKSKSNIETFNLGTGRGLSVLE
                     LVNTFQKVNGVKVPYRIVDRREGDIEKVWADPSYANNELGWTAQETVEETLRTTWKWE
                     LRLAKKAKEQ"
     gene            2151..3125
                     /locus_tag="PKH58_10740"
     CDS             2151..3125
                     /locus_tag="PKH58_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HNX89551.1"
                     /translation="MYPLKFQPIVKDKIWGGHKLQEIFGKQPDGLPNIGESWELSGYP
                     TDASVVANGYLAGKTLPELIEKYRGQLVGERVYNRFGNKFPLLFKLIDANDDLSIQVH
                     PNDDTAGKRHQSFGKTEMWYVLHAEPEASLIIGFTKKCTKKQYLEALEKGEVETLLQK
                     VPVQAGDVFFIPAGLVHAIGRGVMVAEIQESSDVTYRIYDYKRTDDKGNERELHTAEA
                     LDVINFSASKNPKTPYRLTTNADPQLLVSCEYFTTNIMELDKPRRMQNVPDGTFVVYM
                     CLEGSMEISGGTSNVQVKKGETVLIPAGMDVATFIPEGKVRLMEIYIR"
     gene            complement(3323..4192)
                     /locus_tag="PKH58_10745"
     CDS             complement(3323..4192)
                     /locus_tag="PKH58_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89552.1"
                     /translation="MRTRFLFIFIVLSAIITNAQDFAPIGATWHYSYQTINPNIISFK
                     KIESVSDTTINGVLCKKLKENTSNESYYLYSKNDSVFVYKENTFHLLYDFGANAGETI
                     TLGSYYKTYNGLPLKMTIDSVKTILVSGQQRKVQFVTCGDRMIIEFGGQVIQGIGNTN
                     YMFPTLDNAPEGPLRCYQETNSSLFINPYYKNENWNKTDCEQLIVMNYISETETKNSI
                     SVFPNPTNNYLNVNGIELNSKFRIYDTTGREVFQGIFNKSKTVDMKFLENGIYYIEIT
                     NCDTKTVYKFIKK"
     gene            4466..5239
                     /gene="rlmB"
                     /locus_tag="PKH58_10750"
     CDS             4466..5239
                     /gene="rlmB"
                     /locus_tag="PKH58_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNX89553.1"
                     /translation="MINYQFKTKQRPEKEMIFGIRAVIEAIQAGKEIDKILVRRDMTS
                     DLSKELFTAIENRIIPVQKVPLEKLNRITTKNHQGVIAFISPVIFQRIEDIIPLVYEE
                     GRNPFVVVLDGVTDVRNFGAIARTCECAGVDAIIIPARGGAAINADAVKTSAGALMNI
                     PVCREENLRDALKFLANSGLKLVAATEKAAESYTKASLTEPVAILMGSEDEGIAPEHL
                     RLCDSLVRIPILGKIQSLNVSVAAGILIYETVRQRTEVL"
     gene            5321..6004
                     /locus_tag="PKH58_10755"
     CDS             5321..6004
                     /locus_tag="PKH58_10755"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX89554.1"
                     /translation="MTILYEDNHIIAVNKNSSEIVQGDKTGDKPLSETIKTYIKEKYN
                     KPGEVFLGVTHRLDRPVSGVVLFAKTSKALARLNDMFRNQEVKKTYWAIVQNRPKEPE
                     GRLEHYLVRNEKQNKSFAYSDERKNAKKAALTYKLIAASDRYFLLEVQLETGRHHQIR
                     CQLAAMGCPIKGDLKYGFARSNPNAGISLHARSVSFIHPVSKEKIHITAPVPQDDKLW
                     KSFEEMVKE"
     gene            6285..6839
                     /locus_tag="PKH58_10760"
     CDS             6285..6839
                     /locus_tag="PKH58_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX89555.1"
                     /translation="MDQTGIINIVNQCRKGDSSSFRLIVEEYQYMIYQLAFRMLCNED
                     EAKDIVQETFIRAWQNFSKYRPEIRMSTWLYTIATNGCIDRLRHKRNENTDTDMQEQL
                     KYFASNENIEQHLINSEQAQLVMALTCQLTPKQKAVFTLHYLEELSTEEIQKITGLSA
                     EKIKSNLYLARKSIRQKLELLDHE"
BASE COUNT         2093 a         1362 c         1499 g         1903 t
ORIGIN      
        1 aaaatactta tctgatgaaa cctacattat ttgtacttgc agccggaatg ggcagtcggt
       61 atggtggttt gaagcaactc gacgggctgg gacccaacgg ggaaaccatc atggattatt
      121 ctgtatttga tgccgaaaga gccggcttcg gtaaagttgt ttttgttatt cgaaaatcat
      181 tcgaagaaga tttccggaac attgtactga aaaaatacga aggtgttatc gaaactgaag
      241 tggtgtttca ggatatcacc gatgtgcccg aaggctttac gtataacccc gaacgtacca
      301 aaccctgggg taccaatcat gccgtactga tgggaaaaga tgttatccgt gaaccgtttg
      361 ccgttatcaa tgcagatgat ttctatggta aagatgcatt cgcggttatg gctgagtttc
      421 ttcgtggtgt aaccggaaaa cagaatgaat actgtatggt aggttatcgt gtgggaaata
      481 ccctgtcgga aagcggatcg gtaagccgtg gtgtgtgtgt ggtggacgaa aacggcaacc
      541 tgaccaacgt ggtggaacgt acttccatcg aaagaaaagg tgatgatatt gttttcatag
      601 acgaagaagg caaagaagtg gtaatcggcg aaaatacacc ggtatccatg aatatgtggg
      661 gtttcacacc cgattatttc gattattcgc tggatgattt caagaaattc ctcactgaat
      721 acggcaacga actgaaatcg gaattcttca tcccgctggt agtcaataac ctgattgtat
      781 ccggcaaagt aacctgcaaa gtattggact caacagccaa atggtttggg gtaacctatg
      841 ccgaagatcg tgctgaagta gtggctaaaa ttcagaaact gattgacgaa ggcatttatc
      901 ctgctaaatt gtataaatag atcaacctta agaatcttat acataaatca aaataaaata
      961 tgactaaaat tttagtaaca ggcggtactg gctatatcgg ttcgcataca gtggttgagc
     1021 tccagaatgc cggatttgaa gtggttatcg ttgacaacct ctcgaactcc aacatcgaag
     1081 tgttgaatgg tatcgaaaaa attaccggca ttcgtcccgc atttgaaaac attgattgca
     1141 ccgattatgt aaacatggac cgcatgttcg aaaaatataa cggtatagaa gcaataatcc
     1201 attttgctgc cagcaaggct gtaggagaat cggttgagaa accgctgctt tactaccgca
     1261 ataatctgct ttcgctgata aatctgatgc agctgatgcc tatacacaat gttaaaaatt
     1321 tggttttctc ctcatcatgt accgtgtacg gacagcctga tattcttccc gtaactgaaa
     1381 gcgcccccat caaaccggca ctttcacctt acggaaacac aaaacagatt tgcgaggaaa
     1441 tcattcgcga tacagcttat tcaaactctt tctaccaggc tacaatcctg cgttacttca
     1501 atccgattgg cgctcatcca agtgcagaaa tcggtgaact ccccaacggt gtgcctaata
     1561 acctggttcc tttcatcacc cagactgctg ctggtgttcg caaacaactt caggtgtttg
     1621 gtaatgatta caacactcct gacggaacct gtatacgcga ctatatcaac gttgtggacc
     1681 tggccaaagc gcatgtcatt gccgtaaaac gcatgctgga acataaaagc aagtcaaata
     1741 tcgaaacttt caacctggga acaggacgcg gtttgtcggt acttgaactg gtaaatacat
     1801 tccagaaggt gaatggtgtg aaagttcctt accgcattgt tgaccgccgc gaaggtgaca
     1861 tcgaaaaggt ttgggctgat ccttcgtacg ccaacaatga actgggctgg actgctcagg
     1921 aaacggtgga agaaacactg cgcactacct ggaaatggga attgcgactg gcaaagaaag
     1981 cgaaagaaca atagttggcc tctccctaaa tccctctccc cagggagagg gactttataa
     2041 aagcctctac aattctgaag ttggtacaca ttattcttat atctttaagt ccctttcatg
     2101 ggatttatgg gccaaaccaa taaccaataa caattaactt aataaccaaa atgtatccac
     2161 tgaaatttca accgattgta aaagataaaa tctggggcgg acataagctg caggagattt
     2221 tcggaaagca acccgatggt ttacccaata tcggggaaag ctgggagttg tcgggatacc
     2281 cgacagatgc ctcagtagtt gccaatgggt atctggcagg gaaaacgctg cccgaactga
     2341 ttgaaaaata ccgcggacag cttgtgggcg agcgtgttta caaccgcttt ggaaataaat
     2401 tcccgctgct ctttaagcta attgatgcca acgacgattt gtccattcag gttcacccca
     2461 acgatgacac tgccgggaag cgtcatcaat cgtttggtaa aacagaaatg tggtatgtgc
     2521 tgcatgccga accggaagct tcactcatca ttggctttac aaagaaatgt accaaaaagc
     2581 aatacctcga agcacttgaa aaaggtgaag tggaaacact gcttcagaaa gtgccggtgc
     2641 aggccggtga tgttttcttt attccggcag gactggttca tgctattggc aggggagtga
     2701 tggttgccga aattcaggag tcgagtgatg taacttaccg catttacgac tacaaacgga
     2761 cggatgacaa gggtaatgaa cgcgaactgc atacagccga agcgctggat gtgatcaatt
     2821 tctcggcgtc gaaaaacccg aaaacccctt atagactgac aacaaatgcc gatccgcagc
     2881 tcctcgtaag ttgtgaatac tttacgacta atatcatgga acttgacaag ccccgtcgga
     2941 tgcaaaacgt gcccgatgga acatttgtag tgtatatgtg tctcgaagga agcatggaga
     3001 tttccggcgg aacaagcaat gtgcaggtaa agaaaggcga aaccgtgctg atacctgccg
     3061 gaatggatgt tgccaccttt atacccgaag gtaaagtcag gttgatggag atttatatcc
     3121 gataaatcgg ggtctgttaa agcaaaataa ttaaagctgt cacatttcaa atggagacag
     3181 ctttttttat gtcataaaat gaagtacatc gggattgcct cgcgtggcta aatgagattg
     3241 tttttatatc atctttccca ctcgacagat ttgcgcgtta acttgcagta gcagcgattc
     3301 ggtgatatct tatcgaattt gtttatttct ttataaattt atacactgtt ttcgtgtcac
     3361 agttggtaat ttcaatataa taaattccgt tttcaagaaa tttcatgtca actgtcttcg
     3421 atttattaaa aatgccctga aatacttctc ttccagtcgt gtcatagatt cgaaatttag
     3481 aatttaattc aatgccgtta acattcagat agttgttcgt tggattaggg aaaactgaaa
     3541 ttgaattttt tgtttcagtt tcggatatat agttcattac aattaattgt tcacaatctg
     3601 ttttattcca attttcgttt ttgtaatatg gattaataaa cagtgatgaa ttagtttcct
     3661 gataacatct taaaggtcct tcgggtgcat tatctaaagt cgggaacatg tagtttgtat
     3721 ttcctattcc ctgaataacc tgccctccaa actcaattat cattcggtct ccacaagtca
     3781 caaattgtac ttttctttgt tgacctgata ccaatattgt ttttacagaa tcaattgtca
     3841 tttttaatgg aaggccgtta tatgttttgt agtaactccc tagggtaatc gtttcacccg
     3901 cattggctcc aaaatcgtat agtagatgaa aagtgttttc cttataaaca aaaacactgt
     3961 cattttttga atacagataa tacgattcgt tagaagtatt ttctttgagt tttttacaca
     4021 gaacaccgtt aattgtcgtg tctgaaacag attcaatttt cttaaaagaa attatatttg
     4081 gattaattgt ctgataagaa tagtgccatg tagcaccaat gggagcaaag tcctgagcgt
     4141 tagttattat cgctgaaaga acaataaata taaaaaggaa tctcgttctc aaaatgctgt
     4201 attcattgaa atctatatcc tgttttttac gttgggcact aatatcttac attttttacg
     4261 tgcttcaaag ttattgaaaa gaaattaatt tgcaagtttt tacaaaattt tgtttatgca
     4321 gttgaatgaa acggcaagaa agaactgatt tttgctcctt aatgtaaggg aattatgatt
     4381 tttgaccttg ttgttttatc tccgggtgtt gtcattttct ttttcaatct tttcgttgta
     4441 catttgcaaa taaaaaaatc aacaaatgat aaactatcag ttcaaaacca aacaacgtcc
     4501 tgaaaaggag atgatattcg gaattagagc cgttatcgaa gccattcagg ccggtaagga
     4561 aatagataaa attcttgttc ggcgcgatat gaccagcgat ctttccaaag aacttttcac
     4621 agctatcgaa aacaggatta ttcctgttca gaaagttcca ctggaaaagc tgaaccgcat
     4681 caccactaaa aatcatcagg gagtgatcgc ttttatttcg ccggtaattt ttcagcgtat
     4741 cgaggatatc attccactgg tttatgagga gggacgaaat ccgtttgtcg ttgtacttga
     4801 tggtgtgacg gacgtccgaa atttcggggc tattgcccgt acctgcgaat gtgcgggtgt
     4861 ggatgccatc attatccctg ctcgcggagg tgcagccatt aatgccgacg cggtgaagac
     4921 ttcagccggc gcactgatga atattcccgt atgccgcgag gaaaacctgc gtgacgcgct
     4981 taaattcctg gctaattcgg gattgaaact ggtggctgcc accgaaaaag cagcagaaag
     5041 ctatacaaag gcttccctga cagaacctgt agccattctg atgggttcgg aagatgaagg
     5101 cattgcaccc gaacacctgc ggttatgcga ttcgctcgtc agaataccta ttctgggtaa
     5161 aatacagtcg ctcaacgtat cggtagctgc cggaattctg atttatgaaa ccgtaagaca
     5221 acgtacagag gttctttagt aagtgtgtat gcacgatttc atgaagatat tcaatattaa
     5281 cagttcatta ttcccctatc agaggggtta gggagcaagc atgactatac tctacgaaga
     5341 taatcatatc atagctgtca ataaaaacag ttcggaaatt gttcagggcg ataagacggg
     5401 cgataaaccg ctgtcggaaa caattaagac ttatattaaa gaaaagtaca ataaacccgg
     5461 tgaggttttt ctgggtgtaa cgcaccggct ggacaggcct gtgagcggtg tggtgctttt
     5521 tgccaaaacg agcaaagcgc tggcccggtt gaatgatatg tttcgaaatc aggaagtgaa
     5581 aaagacctat tgggcaattg tacagaaccg tccgaaggaa cccgaaggac gtctggagca
     5641 ttatctggtt cgcaatgaaa agcaaaacaa atcattcgct tattccgacg agcgaaagaa
     5701 tgccaagaaa gcggcgctca cctataaatt aattgcagcc tccgaccgtt attttctgct
     5761 cgaagttcag ctcgaaacag gccgtcatca tcagatacgt tgtcagctgg cggcaatggg
     5821 ttgccccatc aagggagatt tgaagtacgg ctttgcccgt tccaatccca atgcaggtat
     5881 cagcctgcat gcccgcagcg taagtttcat tcatccggta tcgaaagaaa aaatacacat
     5941 cacagcacct gttccacagg acgataagct ttggaaaagt tttgaagaga tggtgaagga
     6001 ataaattaaa tatgttcccc agcccttcgg aataatcggg acaggctctg aagggggagc
     6061 ttgaggatat tattctttat aaccatttaa caccccgtta aacaacaatt aattaaactc
     6121 tcataaacag atggatagtt atttctgctg ctcagaaata gtatcatttt cattttacac
     6181 atcgcagtca tttccacttg tcagaaaatt ccgaaaaaaa tctgtattta aattcgactt
     6241 tcgcgcattc cgtccgtata accaatgaag aaaataaaaa gcagatggat cagacaggaa
     6301 taatcaacat cgttaatcaa tgccgcaaag gtgacagttc atctttcaga ttaattgttg
     6361 aagaatatca gtacatgatt tatcagcttg cgttcaggat gctatgcaac gaagatgagg
     6421 ctaaggacat cgttcaggag acatttattc gggcgtggca aaacttttcg aaataccggc
     6481 ctgagattcg gatgagtacc tggctttata cgattgccac caatgggtgc atcgaccggc
     6541 tgagacataa acgaaatgaa aatacagaca cggatatgca agaacagctt aaatattttg
     6601 cttcgaacga gaatattgag cagcatctga taaactcgga acaggcacaa ctggttatgg
     6661 ctttgacgtg ccagctaacg cccaaacaga aagcagtgtt tacgcttcat tacctggagg
     6721 aactcagtac agaggaaatt cagaaaatca ccggattatc tgctgaaaaa atcaaaagca
     6781 atctgtatct ggcgcgtaaa tcaatcagac aaaaattaga attattggat catgaataaa
     6841 gaaaagtttg aaattgt
//
LOCUS       DAOPYB010000164         6852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_13515_length_6852_cov_10.655289, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000164 DAOPYB010000000
VERSION     DAOPYB010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13515_length_6852_cov_10.655289"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..2308
                     /gene="rpsA"
                     /locus_tag="PKH58_10765"
     CDS             197..2308
                     /gene="rpsA"
                     /locus_tag="PKH58_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNX89556.1"
                     /translation="MTEETNLNDQENVQENQEPQENQVETNVDANAQETPAAEVETTE
                     VTEKAETAVAEATAPVAAEEKIAEKPQRIVKPAVDDFDWETIGKKHDNYSGKERKQLE
                     DIYDKTLSSIVEQQVVEGTIVGMNNREVVVNIGFKSDAVVSLNEFRDIPDMKVGDKVD
                     VYVESQENLTGQLILSHKKAKLLKAWDRVNEALEKDEIITGYVKCRTKGGLIVDVFGI
                     EAFLPGSQIDVKPIRDYDIFVGKNMEFKVVKVNNEFKNVVVSHKALIEDELEQQKSQI
                     IAKLEKGQVLEGIVKNITSYGVFIDLGGVDGLIHITDLSWGRINHPEEIVTLDEKIQV
                     VILDFDEGKKRIALGLKQLTPHPWESLDANLKVGDKVKGKVVVIADYGAFIEIAKGVE
                     GLIHVSEMSWSQHLRTAHDFLKVNDEIEAVILTLDREERKMSLGIKQLIPDPWTNIGE
                     RYPVHSKHEAIVRNFTNFGIFVELEEGVDGLIHISDLSWSKKIKHPAEFTKIGEKIEV
                     VVLEVDTENRRLSLGHKQLEENPWDIFESVFMVDSVHKGTIVNANDKGAIISLPYGVE
                     GFAPARHLLKEDNTNARTDETIDFKVIEFSKENKKILVSHTRIWQDAINAEKIKEAAD
                     KKSKDTTAKKVVKKIKDNLEKTTLGDIEALANLKSDMEQTERDNFEKKYKEKQTKKAE
                     KKAEEPKDETKEADGEIKFED"
     gene            complement(2435..4792)
                     /locus_tag="PKH58_10770"
     CDS             complement(2435..4792)
                     /locus_tag="PKH58_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNX89557.1"
                     /translation="MFKRYFFIALILIADLCFAQQAEPLSHVKIWCSDNNHLAAAGIS
                     PVHGQYHHKSYFEGEILSSDLSKLILSGLQYEVVASDLTEFYKSRNQNSMVPELKNII
                     YNCGSPKTYATPSHFSLGSMGGFYTLSEALAQLDSMRAWYPNLISVKSQIGLSIENRP
                     IYSVKISDNPDVNEPEKQVLYTSLHHSSEPCGLQQLIFFMYYLLENYNINSEIKYLVD
                     NLEFYFVPVVNPDGYAYNQTHDPNGGGTWRKNRRDNGLLSYGVDLNRNYGYQWGYNNI
                     GSQPIGSSPWYRGTAAFSEPESQTMRSFIEAHDFLLDMNWHSWGNFLIYPWNYESILT
                     HDSTLFEEFSRYLTIESHYRYGTCDQTYGYNSNGDADDWGYGDTTAKNTIFSFTAEVG
                     SSGDGFWPATANIEALCRNSVDMNLRFAKLGCKFALVNDISPTYISGTTVDIPVEVYC
                     LGLNVPADFTVSITSLSPDIISIGAPATFNVMQTREKRYDDVSFILNPAIAQGTPVTF
                     SLDISNGSYTWKDTITKIFCIFDTLLYDSANNLINWTANDFAVSSATYASSPSCFTES
                     PVGNYGLLQTSSLELTNAIDLSTAVSAYLVFNAKWETEKSYDWVQVFASSDNGNTWQP
                     LCGRYSAYGTDDEEEGEPVYDGFFPNWLQEEIPLNAYVGGSLKIKFEFHSDQTHNFDG
                     FYIDDIAVLSSSMITSDGEIGNTGLISVYPNPAGDQVTIVLSGMDNCSFSIMDIYGNI
                     VVSGLQQPDNNIFQVNTKELASGTYFLKVLSENQTIWTQKLIVIH"
     gene            complement(4878..5708)
                     /locus_tag="PKH58_10775"
     CDS             complement(4878..5708)
                     /locus_tag="PKH58_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX89558.1"
                     /translation="MKRIVCIVILLVSISCLVSGQKFSAGAKAGLNIIPLEKQDLKGN
                     LFSPGYHFGAVGSYKINDWFSLSLEVIYTSQKKIYEKFDTTSFIETLAANPLLSIIGV
                     DINDLLDTLGMVTDYINDDVYNTTRAVVNLAYIKIPVLANFNYKSLYISAGPYVSFLV
                     SNKTTEEYRQHLPLIESFTGLDTIPFFSSIISTSFPGCYEPVTTKIEKDKNIRKVDFG
                     VMAEISYRLNNNFTVGVSYHQGLLNYRSPEIYKKDYLSSINFSIGYLFNLKKNGNSMY
                     "
     gene            6197..6787
                     /locus_tag="PKH58_10780"
     CDS             6197..6787
                     /locus_tag="PKH58_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89559.1"
                     /translation="MGLSIHYSGSFNPAASLQELIEEVRDIAQVYQWECNIYETAFPE
                     QHDGSDYNDKIYGISFRPPDCETVTVEFLSNKRMSTAINLKLFGNSENEDYQKYLYQV
                     SVKTQYAGPAVHMLVVNVFRYLNTKYFLNFKMLDEGKYWETGDETVLKGMFDRYNKLL
                     NMFCDGLECFPVNDGESLESYFERLIEYINKKRQTE"
BASE COUNT         2189 a         1304 c         1450 g         1909 t
ORIGIN      
        1 tcttttcgct aatttagcct catgaattta gttgcgtttg gtagttgaat ctgcgttgaa
       61 aagatttatc tcaattcctt ttgatgtctc ataaatatat tgtacttttg caccccaaat
      121 taatttttta aatcaactta attattaacc cctgtctgtg agtgaattac aggcatacaa
      181 agaaaaaaaa cattacatga ccgaagaaac aaatttgaac gatcaggaaa acgttcagga
      241 aaatcaggaa ccacaggaaa atcaggtaga aaccaatgtt gatgcgaatg cacaggaaac
      301 acctgcagca gaagttgaaa ccactgaagt aactgaaaaa gcagaaacag ccgtcgcaga
      361 agcaactgca cctgttgctg cagaagaaaa gatcgctgag aagcctcaga gaattgtgaa
      421 acctgctgtg gatgattttg attgggaaac tatcggaaaa aaacacgata attattccgg
      481 caaagaacgc aaacaactcg aagacattta tgacaagacc ttaagctcca ttgttgaaca
      541 acaggttgtt gaaggtacca tcgtgggtat gaataacagg gaagttgtgg tgaacattgg
      601 ctttaaatct gatgctgttg tcagccttaa cgaattcagg gatatccccg acatgaaagt
      661 tggagataaa gtggatgtat atgtcgaaag tcaggaaaac ctcaccggac agctgatact
      721 ttctcacaaa aaagctaaat tactcaaagc gtgggatcgc gtgaatgaag ccctcgaaaa
      781 agatgagatc atcacgggct atgttaaatg ccgtaccaaa ggcgggctta ttgtggacgt
      841 atttggcatc gaagcattct tacccggttc acagatcgat gtgaaaccaa ttcgcgatta
      901 tgatattttc gtaggcaaga atatggaatt taaagtggtg aaagtgaaca acgaattcaa
      961 aaacgttgtt gtttctcaca aagcccttat tgaagacgaa ctcgaacaac agaaatcaca
     1021 gatcattgca aaactcgaaa aaggacaggt actcgaaggt atcgttaaaa acatcacttc
     1081 ttacggcgta tttatcgatc ttggcggtgt tgacggcctt attcatatca ccgacctgtc
     1141 atggggacgt atcaatcatc cggaagaaat tgtaacatta gatgaaaaga tacaggtagt
     1201 aattcttgat ttcgacgaag gcaaaaaacg tattgctctt ggcctgaaac aactcacccc
     1261 tcatccttgg gaatcgctgg atgctaacct gaaagttggt gacaaagtaa aaggaaaagt
     1321 tgttgttatc gctgactacg gcgcttttat cgaaattgca aaaggcgttg aaggtcttat
     1381 ccacgtttct gaaatgtcat ggtcacagca tttaagaaca gctcacgatt ttctgaaagt
     1441 caatgacgaa atagaagctg taattcttac cttagaccgc gaagaacgta aaatgtcgct
     1501 cgggataaaa caactgatac ctgatccatg gaccaatatc ggtgaaagat acccggtaca
     1561 ttcaaaacac gaagctatcg tccgcaactt taccaacttc ggtatatttg ttgaactcga
     1621 agaaggagtt gatggcctga tccacatttc tgacctttca tggtcgaaga agatcaaaca
     1681 cccggctgag tttacaaaaa ttggtgagaa gatcgaagtt gttgtactgg aagtcgacac
     1741 cgaaaaccgc cgtttgagct taggtcacaa acaattagaa gaaaatccgt gggatatctt
     1801 cgaatcagtt tttatggttg acagcgttca caaaggaacg attgtaaacg ctaatgataa
     1861 gggcgctatt atctctctgc cttatggtgt ggaaggtttt gccccggcac gccacctgtt
     1921 aaaagaagat aacacgaatg cccgtactga tgaaaccatt gattttaaag tcatcgaatt
     1981 ttcgaaagaa aataagaaga tacttgtttc acataccagg atatggcagg atgctatcaa
     2041 tgctgaaaaa atcaaggaag ctgccgacaa gaaatctaaa gataccactg cgaagaaagt
     2101 tgttaaaaag atcaaggaca accttgaaaa aacaaccctt ggcgatatcg aggctcttgc
     2161 caacctgaaa tccgatatgg aacagacgga acgcgataac ttcgaaaaga aatacaagga
     2221 aaaacaaact aaaaaagctg agaaaaaagc agaagagcca aaagacgaga cgaaagaagc
     2281 tgatggcgaa ataaaatttg aagactaaaa attgtttttc actataatgg aaaagccggt
     2341 tcgtgagagc cggctttttt attttatata gcaggatggc atagccttga taaatgctgt
     2401 ttgttgggat ttcaactcct gaagagcaag cctgctaatg aataacaatt aacttttgtg
     2461 tccatatggt ctggttttct gacaatactt tcaggaaata agtcccggag gcaagttctt
     2521 tggtattaac ctgaaaaata ttattatcag gttgctgcag gccggaaact acgatatttc
     2581 cgtaaatatc cataatgctg aatgaacaat tatccatgcc ggaaagaaca atagtgacct
     2641 gatcaccggc cgggttggga taaacgctta tcaggccagt attgccgatc tccccgtcag
     2701 aagttatcat gctgctcgaa agcacagcga tatcatcaat gtaaaacccg tcgaagttat
     2761 gggtctggtc gctgtgaaat tcaaatttga tctttagcga gccgccaacg taagcattca
     2821 gtggaatttc ttcctgaagc cagtttggga aaaagccatc ataaaccggc tctccttctt
     2881 cctcatcgtc ggtaccataa gcagaataac gcccgcacag tggctgccag gtatttccgt
     2941 tatctgatga ggcaaagacc tgtacccaat catatgattt ttctgtttcc cattttgcat
     3001 taaaaacaag gtaagcactg acagcagtcg ataaatcaat cgcatttgtc agttctaaag
     3061 acgatgtctg aagcagcccg taatttccta caggcgattc tgtaaaacaa gatggggaag
     3121 aagcataggt ggcggatgaa acagcaaagt cgttggccgt ccagttaata agattgttgg
     3181 cagaatcgta caataaagta tcgaatatac agaatatttt ggtgatggta tctttccacg
     3241 tataactgcc attactgata tccagggaga aagttaccgg tgttccctgg gcgatggcgg
     3301 gatttaaaat aaaagaaaca tcatcatatc ttttttcacg tgtctgcata acattgaagg
     3361 tggcaggggc tcctatgctg atgatatcag gtgacagaga cgtaatgcta acggtgaaat
     3421 ctgcaggaac atttagcccg aggcaataca cttctaccgg aatatcaacg gtagtgccag
     3481 aaatataggt aggggagatg tcgttaacaa gagcgaattt gcaccccagt tttgcaaagc
     3541 gcaggttcat atccacagaa tttctgcaca acgcttcaat gtttgctgtt gccggccaga
     3601 aaccatcgcc ggaactacca acttcggcgg taaacgaaaa tattgtattt tttgccgtgg
     3661 tgtcgccata gccccaatca tcggcatcgc cgttgctgtt gtaaccatag gtctgatcac
     3721 aggtgccgta acggtaatgg ctttcaatgg tcagatagcg cgaaaattct tcaaataagg
     3781 ttgaatcatg ggtcaaaatt gattcataat tccatggata aatcaggaaa tttccccacg
     3841 aatgccagtt catgtcaagt aaaaaatcat gtgcttcgat gaatgatctc atggtctggc
     3901 tttccggttc ggaaaaagcc gccgtgcccc ggtaccaggg tgagctgccg atgggctgtg
     3961 agcctatgtt gttatatccc cactggtagc cgtagtttct gttcaggtca acaccgtacg
     4021 acagcaggcc gttgtccctt ctgtttttgc gccaggtgcc gcctccgttg gggtcgtgtg
     4081 tttgattata cgcatagcca tcaggattga caacaggtac aaaataaaat tcaaggttat
     4141 caaccaggta ttttatttcg ctgtttatat tatagttttc cagaagataa tacatgaaaa
     4201 atatcagttg ctgtagtccg caaggctcgg aggaatgatg caacgaggtg tacaaaacct
     4261 gtttctcggg ttcatttaca tcaggattgt cagagatttt taccgaataa atagggcggt
     4321 tttctataga taagccgatc tgcgatttca cagagatcag gttgggatac catgcccgca
     4381 tagaatccag ttgtgcaagg gcttcagata atgtgtaaaa cccacccatc gatcccaaac
     4441 tgaaatgaga gggtgtcgcg taggtttttg gtgagccgca gttgtaaata atatttttta
     4501 gttcaggcac catggaattt tgatttctgc ttttataaaa ttccgtcagg tcggaagcaa
     4561 ctacctcgta ttgaaggcct gacaatatta atttgctcag gtcgctgctg agaatctctc
     4621 cctcaaaata cgacttatgg tggtattgtc catgaaccgg tgaaatacct gccgccgcta
     4681 agtggttatt gtcgctgcac catattttaa catgggaaag tggttcagcc tgctgggcaa
     4741 aacataaatc agcaataagg attaatgcta taaagaagta tcttttaaac atataggtat
     4801 gaatattttt atgccaaaca atggcaggca aagaatcgaa gcacaataaa agagtggact
     4861 gtttcagcat ctttatacta atacatggaa ttaccatttt tcttcaggtt gaataaataa
     4921 cctatggaga aattgatcga cgacaggtaa tcttttttat aaatttccgg cgagcggtaa
     4981 ttgagcaaac cctgatgata acttactcct acagtaaaat tattgttcaa gcggtacgaa
     5041 atttccgcca tcacgccgaa atcaaccttc cgtatgtttt tatctttttc aatttttgtg
     5101 gtaaccggtt cgtagcatcc cgggaacgaa gtgctgataa tggaactgaa aaatggaatg
     5161 gtgtcaaggc ctgtaaagga ttcaataaga ggaaggtgct gcctatactc ttctgtggtt
     5221 ttatttgaaa caagaaaaga gacataaggc ccggcggaga tgtacagcga tttgtagttg
     5281 aagttcgcca gtaccggaat tttaatataa gcaaggttca caacggctct ggtggtgtta
     5341 tatacatcat cattgatata gtctgtaacc attcccagcg tatcgagtaa atcgttaata
     5401 tccacaccaa tgatactcag tagagggttg gcagccaatg tttctataaa agaagtggta
     5461 tcaaattttt catatatttt cttttgtgaa gtataaatta cttctaacga aagcgaaaac
     5521 cagtcgttga ttttataact gccaacagcc ccgaagtgat agcccgggct aaataaattg
     5581 cctttcagat cctgcttttc cagcggaatg atgttcaatc ctgcttttgc cccggcgctg
     5641 aatttctgcc cgctcaccaa acaggaaatg gataccaata ggataacaat gcatactatt
     5701 cttttcatga ttttcaatat tttgtttcgg tacaaaagta ataaagcttg tcattggttg
     5761 ggtaggtttt tatttcagca atgcaattta ggaggtttaa tcggcctagt atgtgatcca
     5821 attaaaagta gtaacttaac cctaaggatt tattaatttt ttacgatggc ttattattac
     5881 aaattatgtt ataaataaaa gtactgtcaa atgcaataaa atgaaggttt tcagtattaa
     5941 taatttatta cttatttata cactgaattt attatccatg aataccataa agtttaccaa
     6001 taagaacaat ttttttctta cgactcccat tattatcatc ccaatatttt cgggtcttca
     6061 ccttttaaag gaaaagcggg ttcgggataa acaaatttat ccttacattt accctcaaat
     6121 aataaatgag atggaaaata tccctgttat tattaatgac aatcaggacg aaaaggaata
     6181 acttacaaaa ttctttatgg gactaagcat acattacagc ggtagtttta atccggcggc
     6241 ttctttgcag gaattaatag aagaagtcag ggatattgca caggtttatc aatgggaatg
     6301 caacatttat gaaacagctt tcccggagca gcatgatggc tcggattaca atgataaaat
     6361 atacggcatt agttttagac cgcccgattg tgaaacagta actgttgaat ttctctccaa
     6421 caaaaggatg agcacggcaa taaatctcaa gctttttggc aattcagaaa acgaagatta
     6481 tcagaaatat ctgtatcagg tttctgtaaa gacccaatat gccgggcccg ctgtacatat
     6541 gcttgttgtt aatgttttca ggtatctgaa cacaaaatat ttcctgaact ttaaaatgct
     6601 cgatgaaggc aaatactggg aaacaggcga cgaaaccgtt ttaaaaggga tgtttgaccg
     6661 ttacaataaa ttgctcaata tgttttgtga tggcttggaa tgtttccctg ttaatgatgg
     6721 cgaatccctc gaatcctact tcgagcggct cattgagtac atcaacaaaa aaagacaaac
     6781 agaataagag attgtttaaa tttcagaact cctgacctgc agcttaaaaa tcaatagttt
     6841 gggttatatt gg
//
LOCUS       DAOPYB010000165         6829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_13578_length_6829_cov_8.192353, whole genome shotgun sequence.
ACCESSION   DAOPYB010000165 DAOPYB010000000
VERSION     DAOPYB010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13578_length_6829_cov_8.192353"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..916)
                     /locus_tag="PKH58_10785"
     CDS             complement(119..916)
                     /locus_tag="PKH58_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNX89560.1"
                     /translation="MKRINYLLLLFITVFSYSCKKDNTEESINWVLTWSDEFAGAAGQ
                     LPDATKWNYDTGGGGWGNNELETYTNNPANVSLDGNGHLVITAKSDLTSARIKTKGLF
                     DLQYGKIEASIKLPAGTGMWPAFWMLGSDIDYNTWPGCGEIDIMEANGAKPTIVHGTV
                     HGPGYSGGSCITSSYTLKSGRIDTAYHLYAVEWSSTDIKFFVDGIQYSKVVPKDVSPS
                     QWVFDKKFFLILNLAVGGNFIANPPVSSITLPKSMYIDYIKVYKNAN"
     gene            complement(983..2311)
                     /locus_tag="PKH58_10790"
     CDS             complement(983..2311)
                     /locus_tag="PKH58_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89561.1"
                     /translation="MKRNKLKYFVQVSGLILAMVITSCSQRDLNSLDPAINSTTAEVY
                     TDGLSGGEDFNAWGKATDGIIDFSNTDEVYSGSKSIKIEVPTATDPDGSWAGGVIFTK
                     GSPRNLTAYNALAFYVKASMATKMTAGFGTPASGPTPHAVTYTNFNVTTVWKKIIIPI
                     PNPSVLTSETGMFNYALPGGTTPYQVYIDDVKYENLGDEFYPVSINCDSVYYFTGDYP
                     VSKMTFTQTFSMPNGDNATFNTSSAYYTLSDIANTSIATMSTPDILSILSAGTTSVKV
                     SAGSFSSTFNLKSTGIVKPPVPTQNASAVSAVVNSGGKYTCNTIDTWWAGATIINAIN
                     VGGHNLFKLSNLDWCCMVMNSSKMNVSTKTKLHLDIMVPATVASKTFIVELKNNDTSL
                     GGQTTNITLAPNTWMPIDLDISGKSPKTAINLIVFSGTIGNVFIDNVYFY"
     gene            complement(2335..5430)
                     /locus_tag="PKH58_10795"
     CDS             complement(2335..5430)
                     /locus_tag="PKH58_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HNX89562.1"
                     /translation="MKRNLFIIFLFLSSLSMFSQTQKVKVLQNDKGIKLQVDGKDFII
                     NGMNWDYFPIGTNYNYSLWKQSDDFIKTALDNEMSMLKNMNVNTIRAYVGIQPKWIKY
                     IYENYGIYTVVNHTFGRYGLNVEGVWYGVTDYSDPKVIKMLNNEVSNMVKTYQGTPGL
                     LMYLLGNENNYGLFWDGAETEDIPINDRKSTRRAEALYKMFEQGVLTAKKIDTNVPVS
                     ICNGDALFIDIIAKECPDVDVLGINSYRGISFTDIFTKIKAACPNKPIMFTEFGGDAY
                     NAQTQTEEQRGQAVFDLANWKEIYENAAGMGGTGNAIGGFTFQFSDGWWKTKMTVDLD
                     VHNTISTWNNGGYSYDYVPGTNNMNEEWFGICAKGETDALGHYQLYPRAAYYALQDAH
                     KFNPYTSGATRDALEKHFSNIQIQGEILKARGDKSALDNEQTKKYSISIRGEISTFNT
                     GGKNITTPPSKYASETAIPNKLGFDHMESYYLGFDAKPSDNVRANVTLNVIGNVAKNA
                     IDEIFYENKAREAVFVNPQTGEKYDLNLGKHISIYKAEYSWDTKWYNLKGFYRTGHFH
                     WGYEGDFFGLYPETNYGPNLDIYHGEAPFGFEVAGKKGLDGFKIAFGPELWWGANPAV
                     LAKYQKELGKFTLAAIYQEDLDRKAYSTSSQVQPQQQTRKATLYAKTKVGLLGVEAGG
                     IWAGNPLNGMSFNIWDADNQRLYQDVVSARDNWGGKVKFTLQSGRINWYLQAAAKGLV
                     AEGGADWTKTFTGWRLKDCGSGNQYNVLTGLAYNLGFFQIAPNFLYQKPFVAAIPEGA
                     VDSQTGVVGHSRNIKFDPFSVRGNRETVAGELLLAYDPTPATWMWEWDADVQEDATFA
                     GSIDFVYRYQPTQMDATTGYSANGTPYALAAPPAHDVWEINSHLVSKLTNDFGIIVNL
                     LTGTAQPNGPDARIVTRYGGDVSVIYNKIKCLGALKVNDYGPYDYHRDYNLTYPLQAM
                     LDLSTYITKPKWFDMPDTKLGLRFTYRTLDQYSNRYDITANGNGNEWEIRTYVHFNIG
                     K"
     gene            complement(5477..6403)
                     /locus_tag="PKH58_10800"
     CDS             complement(5477..6403)
                     /locus_tag="PKH58_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89563.1"
                     /translation="MKKNEKIDFPFGKSICYSGFRDKQAPGEKYPSYDEVKQDLLILQ
                     KHWNYLRMYDCDEHAETVLNVIRNEKLNFKVMLGAYIVAEMNNFGCPWGGYFSEEELM
                     ENKEKNIQQIEKLIALANSYSEIIFSLSAGNEACVDWTDHYVSEDSVIKYVRMIKEGA
                     KQPVTFCENYVPWVTKLKRLAEEVDFISIHTYPVWEYKNIHEALSYTQQNYNAVADAY
                     PHKLVIITEAGWATNSNGRGILPDNVSEENQMIYYNDLMDWAEKEKKIIFVFEAFDEP
                     WKGSPEPLEPEKHWGLYNVDRSPKLVMKQLHK"
     gene            complement(6422..>6829)
                     /locus_tag="PKH58_10805"
     CDS             complement(6422..>6829)
                     /locus_tag="PKH58_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HNX89564.1"
                     /translation="VGYVDAYYEFTARPNITKACDVILANCYPFWESCQADYSLMYMK
                     EMFYQAVHACDGDKNVIITETGWPSQGEDYKGALPSPENALKYFINTQLWSKQDDVEI
                     FYFTSFDESWKVGAEGDVGAFWGIWDKNEQLKY"
BASE COUNT         1847 a         1405 c         1284 g         2293 t
ORIGIN      
        1 aacttaatgt agaggcaaag aacattacga taaaaagaaa taaactagta attgatttca
       61 taagcgattt gttttttttt gttaaataat ttggttaatt aaatgagaaa taattctgtt
      121 aatttgcatt tttataaact ttaatataat caatatacat tgactttggc aaagtgatag
      181 aagatacggg cggattagct ataaagtttc caccaacggc taaattgaga ataagaaaaa
      241 actttttatc aaacacccac tgacttggag atacatcttt cggtactact tttgaatact
      301 gtattccatc cacaaagaat tttatatcag tactgctcca ctcaacagca tacaaatggt
      361 aagcagtatc aattctaccc gatttcaagg tgtaagatga tgtaatacac gagcctccgg
      421 agtaacccgg tccgtgaacg gttccgtgta caatggttgg tttagcgcca tttgcctcca
      481 taatatctat ctcgccacaa ccgggccacg tgttatagtc gatatcgctg cccagcatcc
      541 aaaacgcggg ccacattcct gtgccggcag ggagtttgat agaggcttca atcttaccgt
      601 attgcaggtc aaacaagccc ttggttttga tacgagccga tgttaaatcc gattttgcgg
      661 taattactaa atgtccgttt ccatccaacg atacgttagc tgggttattg gtataggttt
      721 ccaattcatt gtttccccat ccacctccac cggtgtcgta gttccatttg gtggcatcgg
      781 gcaactgacc ggcagcgccg gcaaactcat cactccaggt taatacccaa ttgatgcttt
      841 cttcagtatt atctttttta cagctgtaag aaaatactgt aataaagagt aataacagat
      901 aatttatacg tttcatattg gtgtaaattg agccaatatt gctttgttaa ccaattcaat
      961 attggctcat tgatgttatt tgttaataga aatacacatt atcgataaat acatttccaa
     1021 tggttcctga gaacacaatc agattgattg ctgtctttgg cgatttacct gaaatgtcaa
     1081 gatcaatagg catccaggta ttaggggcta atgttatatt ggtggtttgc ccgcctaaac
     1141 ttgtatcatt gttttttaat tcaacgataa atgttttgga agcaacagtg gcaggtacca
     1201 taatatccaa gtgtaattta gtcttcgttg aaacgttcat tttactggag ttcataacca
     1261 tacagcacca atctaaattt gaaagtttga acaaattgtg tcctccaaca tttatagcat
     1321 ttattatggt tgcaccagcc caccaggtgt caatagtatt acatgtgtat ttacctccgg
     1381 aatttaccac cgcagaaact gctgatgcgt tttgtgttgg taccggtggt tttacaatac
     1441 cggttgattt caaattgaaa gtagatgaaa aactaccggc tgaaactttt accgaggttg
     1501 tacccgcact caaaatactt aaaatatctg gagttgacat cgtagcaata ctggtattgg
     1561 caatgtctga caaggtataa taagccgacg atgtattgaa agtagcatta tcgccattag
     1621 gcattgagaa agtttgtgtg aaggtcattt tacttacagg ataatctcct gtaaaataat
     1681 acacggagtc acagttaatg gatacaggat aaaattcatc acctaaattt tcatacttaa
     1741 catcatcaat ataaacctga tatggagttg ttcctcccgg cagtgcgtaa ttaaacattc
     1801 ctgtttcact cgtaagtacc gacggatttg gaattggaat aattattttt ttccatacgg
     1861 tagtaacatt gaaatttgta tatgttacag cgtgaggagt aggtcccgaa gctggagtgc
     1921 caaatcctgc agtcatctta gttgccatac ttgcctttac gtaaaaagct aaggcattgt
     1981 aagcagtgag attacgtggt gagccttttg taaaaatcac cccgcctgcc cagctgccat
     2041 cggggtcagt tgcggtaggt acttctattt taatggattt tgatccgcta tacacttcgt
     2101 ccgtattact gaaatcaata atcccatcgg tagcttttcc ccaagcatta aaatcttcac
     2161 ctcctgacaa gccatcggta tatacttctg ctgtggtaga gttgattgca ggatccaacg
     2221 agtttaaatc tcgttgactg caacttgtta ttaccattgc aagtatcaat cccgatactt
     2281 gcacaaaata ttttaattta ttgcgtttca taatttttag ttattaaaat ttgattattt
     2341 accaatgttg aaatgaacgt aagttcttat ttcccactcg tttccgtttc cattggcagt
     2401 tatatcatat ctgttggaat attggtcaag tgttctgtaa gtaaatctta agccaagttt
     2461 tgtatccggc atatcgaacc attttggttt ggtaatgtat gttgataaat ctaacattgc
     2521 ctgcaaagga tatgtcagat tataatcgcg gtgataatca taaggaccat agtcatttac
     2581 tttaagagct cccaagcatt taattttatt gtaaattaca cttacatcac caccgtagcg
     2641 tgtaacgata cgtgcatcgg gtccgtttgg ttgggctgtt ccggttaata aatttacaat
     2701 tattccaaag tcatttgtaa gtttagaaac taaatgtgaa ttaatctccc acacatcgtg
     2761 cgcaggcgga gctgccaaag cataaggtgt tccgttagcc gaataacctg ttgtagcgtc
     2821 catttgtgtg ggttgatagc ggtacacaaa atctatactt ccggcaaaag ttgcgtcttc
     2881 ctgaacatcg gcatcccatt cccacatcca tgtggcgggt gttgggtcat acgctaaaag
     2941 caattcaccg gcaacggttt cacggttacc tctcacggag aaaggatcaa atttgatgtt
     3001 tctggaatga cctacaactc cggtttgtga atctactgca ccttcgggta tggctgcaac
     3061 gaatggtttt tggtacaaga agttaggagc aatttggaaa aatcccagat tgtatgctaa
     3121 acctgtcagt acattgtatt ggtttccact tccgcaatct ttaagtctcc atccggtaaa
     3181 tgtttttgtc cagtcggctc caccttcggc tactaaacct tttgcggctg cttgcaggta
     3241 ccagttaata cgcccgcttt gtaaagtgaa tttcacttta cctccccagt tgtctcttgc
     3301 cgaaactaca tcctggtata atctttgatt atcggcatcc cagatattga atgacattcc
     3361 gttcaaagga tttcctgccc atatgcctcc tgcttctacg cccagtaagc ctacttttgt
     3421 tttggcatat aaggtagctt tacgagtctg ctgttgtggt tgcacttgtg atgaggtact
     3481 gtatgctttt ctgtctaaat cttcttgata tatggctgca agtgtgaatt tacccagctc
     3541 tttctgatat tttgctaaaa cagctgggtt agctccccac caaagttcgg gtccaaacgc
     3601 tattttaaaa ccatccagtc ctttttttcc tgctacttca aatccaaatg gcgcttctcc
     3661 gtgataaatg tccaagtttg gtccgtagtt tgtttcggga tacaagccaa agaagtcgcc
     3721 ttcgtagccc caatggaaat gtcctgtacg ataaaatcct ttcagattat accatttggt
     3781 atcccaacta tattctgcct tgtaaataga aatatgtttg cctaaattta agtcgtattt
     3841 ttcaccggtt tggggattta caaacacagc ttctcttgct ttgttttcat agaatatttc
     3901 atcaatagca ttttttgcca catttccaat tacatttaac gttacattgg cgcgtacgtt
     3961 gtcacttggt tttgcatcaa agccaagata gtatgattcc atatggtcaa atcctaattt
     4021 attgggaatt gcagtttctg aagcgtattt tgatggaggt gtggtgatat ttttccctcc
     4081 tgtgttgaaa gttgaaattt cgccacgaat gctgatgctg tatttttttg tttgttcgtt
     4141 gtctaaagcc gatttgtcac ctctcgcttt caagatttcg ccttgaattt ggatattgct
     4201 gaaatgtttt tccaaagcat ctctggttgc acccgaagta tatggattaa atttatgtgc
     4261 atcttgcaaa gcataatagg ctgcacgtgg gtagagctgg taatgtccta aagcgtctgt
     4321 ttcgcctttg gcacagatac caaaccattc ttcgttcata ttattggtgc cgggcacgta
     4381 gtcataggag taacctccat tgttccaggt agaaatggta ttgtgtacat ccaagtcaac
     4441 agtcatctta gttttccacc atccgtcact aaattggaag gtaaaccctc cgatggcgtt
     4501 tccggtgcca cccattccgg ctgcattttc gtaaatttcc ttccagttgg caaggtcaaa
     4561 tacggcttgt ccgcgttgtt cttctgtttg tgtttgtgcg ttgtaagcat cgcctccaaa
     4621 ctcggtaaac ataatgggtt tgtttggaca agcagctttg attttggtga aaatatcggt
     4681 aaacgaaata cctcggtagg aatttattcc caatacatcc acatcgggac attctttggc
     4741 aataatgtca ataaacaagg catccccgtt acagatagaa accggtacat tggtatctat
     4801 ctttttggct gtgagtacac cctgttcaaa cattttatac aatgcttcgg ctctgcgagt
     4861 tgatttgcgg tcatttatcg ggatgtcttc tgtttcggct ccgtcccaaa acaaaccgta
     4921 gttgttttcg ttacccaata aatacattaa caaaccggga gttccctgat aggtttttac
     4981 catatttgaa acctcgttgt tgagcatttt tatcactttc gggtcggaat aatcagtaac
     5041 cccgtaccat actccctcta catttaaacc gtaacgcccg aaggtgtgat ttacgactgt
     5101 gtatattccg taattttcat atatgtattt aatccacttt ggttggatgc ccacatatgc
     5161 gcggatggtg tttacattca tgtttttcaa cattgacatt tcgttgtcaa gcgccgtttt
     5221 gataaagtca tcggattgtt tccacagact ataattatag ttggtgccaa tcgggaaata
     5281 atcccaattc ataccattga tgatgaaatc ttttccatca acttgtagtt ttatcccttt
     5341 atcgttttgc aataccttta ctttttgggt ttgcgaaaac attgataaag aagacaaaaa
     5401 aaggaaaatg ataaataagt ttcttttcat ggttcttact taaattttgt gttaataatt
     5461 taatttggtt taatctctat ttgtgtaatt gtttcattac aagttttggg cttctgtcca
     5521 cattgtacag cccccaatgt ttctccggtt ccaatggttc gggtgagcct ttccaaggtt
     5581 cgtcaaacgc ttcgaataca aaaattattt ttttctcttt ttcagcccag tccatcaaat
     5641 cgttgtaata tatcatttgg ttttcttcac ttacattatc gggcaatatg cctctgccat
     5701 tggagttggt tgcccagccg gcttcggtta ttatcaccaa tttgtgagga taggcatcgg
     5761 caacagcatt gtaattttgc tgcgtgtagc tcagcgcctc gtgaatattt ttgtattccc
     5821 aaacggggta tgtatgaatg gatataaaat ctacctcctc cgccaaacgt ttgagtttgg
     5881 taacccaggg cacgtagttt tcgcaaaaag tgaccggctg ttttgctcct tcttttatca
     5941 ttcttacata ctttattaca ctgtcttcgg acacgtagtg gtctgtccag tcaacgcagg
     6001 cttcgtttcc tgccgaaagc gaaaaaatga tttcagaata agaatttgcc aatgctatca
     6061 gtttttctat ttgttgaatg tttttttctt tattctccat caactcttct tctgaaaaat
     6121 aacctcccca ggggcatcca aagttgttca tctctgcaac gatgtacgca cctaacatta
     6181 ccttgaagtt taatttttca ttcctgataa catttaaaac cgtttcggcg tgttcatcgc
     6241 aatcgtacat tcgtaaataa ttccaatgtt tttgaagtat caataaatct tgctttactt
     6301 cgtcgtagct tggatatttt tcaccgggtg cttgcttgtc tctaaaacct gaatagcata
     6361 ttgatttccc gaaaggaaaa tctattttct catttttttt catcaaacgc ctaaaaaagt
     6421 attagtattt aagttgctca tttttgtccc atattcccca gaacgcacct acatcacctt
     6481 cagcacctac tttccacgat tcgtcaaaag aggtaaagta aaaaatctct acatcatcct
     6541 gttttgacca gagttgtgtg ttgatgaaat attttaatgc attttccggt gatggcagcg
     6601 cacctttata atcctcaccc tgacttggcc agccggtttc agttataatt acatttttat
     6661 cgccatcgca ggcgtgaacc gcctgataga acatttcttt catgtacatc aaggaataat
     6721 cggcctgaca actttcccag aaagggtaac aattggctaa aatgacatcg caggctttcg
     6781 ttatgttggg acgggctgtg aattcataat aggcatccac ataccccac
//
LOCUS       DAOPYB010000166         6766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_13734_length_6766_cov_10.049024, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000166 DAOPYB010000000
VERSION     DAOPYB010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13734_length_6766_cov_10.049024"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKH58_10810"
     CDS             <1..794
                     /locus_tag="PKH58_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89565.1"
                     /translation="LNAAPTAYAVTGSGSYCQDGSGLAVGLSNSDIGVNYQLYSGETA
                     VGSPVSGTGSAISFGNQTAGTYTVTATNATTLITGSMTGSAVITANPPVTPDVSIEAS
                     SNPAATGASVTFTPTTVNGGINPAYEWFVNNVSVGTGETYSYIPTDGDQIYTKMTSND
                     TSPCLTGNPATSNTITMSVSPGTGFSNEKALRINTYSTNKAIYIDCPIKIKQLSVFNM
                     LGSVVAMEENVSGLRKINLNNFPNECYIVKVITDNNVTTQKILLK"
     gene            complement(912..1349)
                     /locus_tag="PKH58_10815"
     CDS             complement(912..1349)
                     /locus_tag="PKH58_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding protein"
                     /protein_id="HNX89566.1"
                     /translation="MKKLSYIFIIVMTIVMASCEQNLEYENLPANKVEGEWMVKAYIN
                     GTNVFGTFPVRTYTTASGKDSIYINDYEGKFWDFNVKAAVKTADNTFQTQHSVSTVTG
                     KSTGVKVLNGRIINNDSIYFEMQFEDDETPYGVSYQLTGHRIK"
     gene            complement(1370..4018)
                     /locus_tag="PKH58_10820"
     CDS             complement(1370..4018)
                     /locus_tag="PKH58_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX89567.1"
                     /translation="MRKFSVLGVLIFSTLSFLSYGQGSTYTGSYVSSSRMEYSAKTNM
                     VIEGKSFNNTTGHSITLWNCSDVTIRNCKFTASPKYLGIYLFGCKNITITNCTFENLA
                     SAMLASTCTGNIKFEYNDVKNILGTSQGGDIAGSMVQFIRVTGAGNSVSYNACENIPG
                     ESAPEDLINMNDGSAGTPESPIMITNNWIRGGGPSPSGGGINLGDVNGSYQTAENNVL
                     VDPGQYGIAISGGHHNVLRNNKVYAKQQSFTNVGIVGCNWYEDIAASHDNVITNNEIN
                     YKNSSGQLNNWWIAANVGIIAGQGTNIYNASLNAAILPAQLLGRALVNTAPTYVWNQT
                     SPASFAVASNWTPARTSPTAKDVLQFSAGGSVVVNNVPTQAIDQLQVLNNSTVELQSA
                     TSTVLTVSNMTINAGSNLKVGAGSQITVATTLTNNGNVSLLSDNTKGTASLLTPATLA
                     GTGTYNIQQYLTDGLNCHISSPVTAALISAINTATSVTTYSEPTAKWVTATGSMTTLK
                     GYLANLTITNGTIAFNGTINNGNLSLPLTRTSNTTNAGFNMLGNPYPSYLNLNNLSTA
                     DIEPTIWYRSKDATSLFDVYNIKSGVFTSNSGLAVTGVVAPMQAFWVKVRKGKTSSTF
                     TINNAQRTHRDVADNVFRAPAANNAERQLIRLIASNGTNSDETVIYTDASASNSYDDY
                     DSSKGMNGSTSKVPDIYTTIDNENLVINGMNSLPLDTEIPLTFAANNAMSTTFSIKAN
                     EITNLASGVSVILKDLETGNETNLTDGTTSYDFTAEAGTTKALSLIFRTITVTTGINT
                     GNNMLVDEQATTIYKNNRNIIFVNCNGGVASDATVSVFNAAGQKLAFQKIKSYHTVVE
                     ATLTSGDYNVTVNNGQKVKTQKISIN"
     gene            complement(4784..5344)
                     /locus_tag="PKH58_10825"
     CDS             complement(4784..5344)
                     /locus_tag="PKH58_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX89568.1"
                     /translation="MKLYIKYMLSVQCKKEMQDQLDKLGLHYGEMEKGELEITEDINY
                     DQLETLKSGLVSAGLELIDDKKALLVQKIKNLINETIEKTELQPKTNLSVYLSDKLNY
                     DYPYLSGLFSEVTGVTIEQYTINQKIERVKELLIFDELNLTEISYLLNYSSVSHLSNQ
                     FKKVTGLTPSFFKYMKQRKMIIRDIG"
     gene            complement(5451..>6766)
                     /locus_tag="PKH58_10830"
     CDS             complement(5451..>6766)
                     /locus_tag="PKH58_10830"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX89569.1"
                     /translation="FVAGQGSAYTGLYKKSPSLRYVDTKNLIIEGVEIVDVKDPCIAL
                     YGCSHITIRNCKLGPAISNLGIYLYRCSDITITNCVIENVASGMIASTCTGNIRFEYN
                     DVKNILGYSRGGEKSWGQMVQFISVSGAGNSISYNVCENVSGESAPEDIINLFNSHGR
                     VESPIRVVGNWLKGGGPSESGGGINLGDNGGSYQTAEENILVDPGQYGIGISGGDHLK
                     IKNNKIVGNKLPFSNVGITVCNWYTNEDVKCQDIEVADNEINYVNKNGEANPLWNGGN
                     CGKIDGWETNLFNRELNKSILPPQIWGRAHQKAPDWTPGWPVVSAIMSDGFTLCVNTL
                     DKGISYYVILPAGSASPTSAQIKEGRDTSGKPLGWNVKGKIKCDIAQTEYEASIPLLA
                     ENKSYDIWFTVENPEYCILGPPVHITVNTLEKNNTINPVSGYINH"
BASE COUNT         1878 a         1364 c         1309 g         2215 t
ORIGIN      
        1 ttcttaatgc cgctccaaca gcttatgccg ttaccggatc aggttcttat tgtcaggacg
       61 gatcaggtct tgcggtcgga ttgagcaatt cggatattgg tgtaaattat cagctttaca
      121 gtggtgaaac tgcagttggg tcaccggttt caggaactgg ttcggctatt agttttggaa
      181 atcaaactgc gggtacttat actgttaccg ccactaatgc tacgacatta atcacaggct
      241 caatgaccgg aagtgcagtt attacggcca atccgccagt cacaccggat gtaagtattg
      301 aagcaagttc caacccggct gcaacaggag cttctgttac ctttacccca acaacggtga
      361 acggcggaat aaatcctgct tatgaatggt ttgtaaacaa tgtttcagtt ggaaccggtg
      421 aaacctattc atatataccg actgacggag atcagattta tacaaaaatg acttcaaatg
      481 acacctcacc ctgcctgacc ggaaatccag caacatccaa taccatcacc atgtcggtaa
      541 gtccgggaac cggattttca aatgaaaaag ctttaagaat taatacttat tccaccaata
      601 aagccatcta cattgactgc cctattaaga tcaaacaact gagtgttttc aatatgctgg
      661 gttctgtggt tgccatggag gaaaatgtca gcggactgag aaagattaac ctgaataatt
      721 tccccaatga atgttatatc gtaaaagtta ttacggataa taatgtaacg acacagaaaa
      781 ttcttttaaa ataattgttc aataactttt atacctggaa ataatcagcc cccaaatcac
      841 ctgacaggtt ttttgggggc tgacggttta tataaactgc taatttataa ccgattgata
      901 catatgatgc attattttat acggtgacct gtcaactgat agcttacgcc atagggtgtt
      961 tcatcgtctt caaactgcat ttcgaaataa atactgtcat tattgatgat acgaccattc
     1021 agcactttca ctccggtact ctttcctgtt actgtgctca cagagtgttg tgtctggaat
     1081 gtgttatcgg cagtttttac tgcagctttc acattaaaat cccaaaattt tccttcgtaa
     1141 tcgttgatat agattgagtc tttgcccgat gcggtagtgt aggttctgac aggaaaagtg
     1201 ccgaacacat tggtaccgtt gatataagct ttcaccatcc attcgccttc cactttattg
     1261 gccggtagat tttcgtattc caggttctgt tcgcatgatg ccatcactat ggtcatcact
     1321 attatgaaaa tataacttaa cttcttcatc tgctgtggga ttctatttat taattaattg
     1381 aaattttttg agttttaact ttttggccat tattcacagt tacgttgtag tctcccgatg
     1441 tcagggtagc ttcaactacc gtgtggtaac tttttatttt ctggaatgct aatttctgac
     1501 ctgctgcatt gaatacggat actgtggcat cagatgcaac gccaccgttg cagttaacga
     1561 aaataatatt gcgattattt ttgtaaatgg tggtagcttg ttcgtcgacc agcatattgt
     1621 tgcccgtatt aatgcctgta gtaactgtta tggttctgaa tatcagactt aaggccttgg
     1681 tagttccggc ttcagctgta aaatcatagg atgtggttcc atcggtcagg ttagtttcat
     1741 ttccggtttc aaggtctttg agaattacac ttacccccga tgccagattg gttatttcgt
     1801 tggctttgat tgaaaatgtg gtcgacatag cattgttagc agcaaaagtc agcggtattt
     1861 ctgtatccag tggaagtgag ttcatcccgt ttatcaccag attttcatta tctatggtag
     1921 tgtaaatatc cggtactttt gaagtgctgc cgttcattcc ctttgatgaa tcgtaatcat
     1981 cgtaactgtt ggaggctgaa gcatcggtat aaattacagt ctcatccgaa ttagtgccat
     2041 ttgatgctat cagtcgtata agttgtcttt cggcattatt ggccgcagga gctctgaata
     2101 cattatcggc cacatcgcgg tgagtgcgct gagcattgtt aattgtaaaa gtagaagacg
     2161 tttttccctt tctaaccttt acccagaatg cctgcatggg agctaccacc cctgttacag
     2221 ccagtccgct attacttgta aacacaccgc ttttgatatt ataaacatca aacaatgaag
     2281 tggcatcctt gcttctgtac caaatggtgg gttcaatgtc agccgtcgat aagttattca
     2341 gatttagata tgaaggatag ggattgccca acatgttgaa accggcattt gtggtgttcg
     2401 atgtacgtgt caacggtaat gaaagattac cgttatttat tgttccgttg aaagcaattg
     2461 ttccatttgt tatggtcaaa ttggccagat agcctttcaa tgtagtcatt gagccggtag
     2521 cagtcaccca ttttgctgta ggttcactgt aagtagtaac actggtagcg gtattgatag
     2581 ccgaaattaa agcggcggtt accggactgg atatgtggca attaagtcca tctgtgagat
     2641 attgttgaat gttgtaggta ccggtacctg cgagggtagc cggagtgagc aacgaagctg
     2701 ttcctttggt gttatctgac agcaaactca cgttgccatt attggtaagt gtggttgcca
     2761 cggtaatttg actgcctgcg ccaactttaa ggttagagcc tgcatttatt gtcatattac
     2821 ttactgtcaa aacggtagaa gtagctgact gtaattcaac tgtggaatta ttcaatacct
     2881 gcagttgatc aatagcctgt gtcggtacat tgttaacgac tacactacca ccggcgctaa
     2941 actgaagtac atccttggct gtaggacttg tgcgggcagg tgtccagttg gatgcaacgg
     3001 caaatgaagc cggagaagtc tgattccaca cataggttgg tgcagtatta acgagtgctc
     3061 tcccgagcaa ttgtgcagga agtatggctg catttaagct tgcattgtat atattggtac
     3121 cctgaccggc aataattccc acattagcag caatccacca gttatttaat tgaccgctgc
     3181 tgtttttgta atttatttca ttgttagtga tgacattatc atgtgatgca gcaatatctt
     3241 cgtaccagtt gcagccaaca atacccacat tggtaaaaga ttgttgtttg gcatacactt
     3301 tattgtttct caacacatta tgatgtccgc ctgaaatggc tatgccgtat tgaccgggat
     3361 caaccaaaac attattttcg gctgtctggt atgagccatt cacgtcaccc aggtttatac
     3421 cacctcccga aggtgaagga ccgccaccgc gtatccagtt attggtaatc ataatcgggc
     3481 tttcgggagt acctgccgat ccgtcgttca tattgatgag atcttccggg gcactttcgc
     3541 cgggaatatt ttcgcaggca ttgtaactta cgctgtttcc tgcaccggta accctgataa
     3601 actgaaccat cgatcctgct atatcgcctc cctgcgaagt tcccaatatg tttttcacat
     3661 cgttgtactc aaatttaata ttaccggtac atgtagacgc caacatggca gatgcgagat
     3721 tttcgaatgt acaattggta atcgtaatat ttttacaacc gaataaatag atacccagat
     3781 atttagggga cgcggtaaat ttacaatttc gtatagtaac gtcactgcaa ttccaaagcg
     3841 ttatgctatg accggtggta ttattaaatg acttaccttc gataaccata tttgttttag
     3901 ccgagtactc catacgcgaa gaacttacgt aagagccggt gtaagtggat ccctgaccat
     3961 aagacaggaa tgagagcgtt gaaaatatca gtacacctaa taccgaaaat tttctcatca
     4021 gttggtttct tttcatcatg ttaaaattaa ttgttattta tttaattgtt cttaaaatta
     4081 tctctttgga cacaaaatct cccctatcaa atgtattgaa tttaactata atcctcaata
     4141 tgtgttattt acacatacga aagtctttat ggtttaggaa atttgcgtga aaaaattaag
     4201 gtaggtatga ttcataaaat tcatgtgatt tcaaaaaaac gtctggtttt tttcccttca
     4261 tttattctat ttctttgcaa tgcagacaac caaacagaga aaaattcaaa acttaaaacc
     4321 gtgaataatt taatttattg tatatctgat aattattaac cttgttaaat cttagcgttc
     4381 gttgaatagc tcaattcgtt ccgatgattt taataaattt attctgtaca aaattagtag
     4441 gttttatttg ttaataaatt acacaatttc ggaaatgatt tgcataattt tcagttcatc
     4501 aaccatttaa cacatctgta aaatataata actttttatt tatgcagcat tctgcgagcg
     4561 aatgctccat aaaaaaataa aacctgacaa aagtattttc ttttgcaggt tcaataatta
     4621 cacaatttca gaaatttatt acacaatttt cagttattca ggatattaaa accaactatc
     4681 cgcaaaattg caataataca gggtactatt gatgaacaga tggagatcca tattgactga
     4741 aaagggcaaa aaaggggcag aaacaccaaa aaaagcagtt caatcatcct atgtcgcgaa
     4801 ttatcatttt tcgttgtttc atatatttga aaaatgacgg ggttaatcct gtaacttttt
     4861 tgaattggtt ggaaaggtgt gacacgctgc tataattgag caggtatgaa atctccgtga
     4921 ggttcaattc gtcgaatatg agtaattctt ttacccgttc tattttttgg ttaatggtgt
     4981 attgctcaat agtaactccg gtaacttcgg aaaacagacc ggaaagataa gggtaatcat
     5041 aatttaattt atcgctgaga taaaccgaca aattagtttt tggctgcaat tccgtttttt
     5101 cgattgtttc gtttatcagg ttttttattt tttgaacgag cagtgctttt ttatcatcta
     5161 tcagttccag tccggcgctg accagacctg atttcagggt ttccaattga tcgtaattga
     5221 tatcctctgt tatctccagt tcaccttttt ccatctcacc atagtgaagt ccgagtttat
     5281 ccaactgatc ctgcatctcc tttttacact ggacgctgag catgtattta atataaagtt
     5341 tcatgtgttt tttggcttaa tatttgattg atataaggca ttgtttctgg atttagctga
     5401 ttaatcccct aattgaaaaa tatctaacaa aataatgaac gaagattgtg tcagtggttt
     5461 atatatcctg atacagggtt tatggtatta tttttttcta aagtgttaac tgtgatgtgc
     5521 actggaggtc ccagtatgca gtattcagga ttttccacgg taaaccaaat atcataactc
     5581 ttattttcgg ccaataatgg tattgaagcc tcatattctg tttgagcaat atcgcactta
     5641 atttttcctt tcacattcca tccgagcggt tttccggatg tgtccctgcc ttctttgatc
     5701 tgagctgatg tgggtgaagc agaacctgca ggcagaatca cataatagga aattccttta
     5761 tccagtgtat tgacacagag tgtaaatcca tccgacataa tggctgatac aaccggccat
     5821 cccggtgtcc agtcaggagc tttttgatgt gctctccccc atatttgtgg cggcagtatg
     5881 gacttattta attcccggtt gaataaattg gtttcccagc catcgatttt tccgcagtta
     5941 cctccgttcc agagaggatt tgcttctcca tttttgttta cataatttat ttcgttatca
     6001 gccacttcaa tatcctgaca ttttacgtcc tcattcgtat accagttaca tacagtaatg
     6061 cccacattac tgaatggcag cttattacca actattttat tgtttttgat tttcagatga
     6121 tctcctccgg aaatgcctat tccatactgc ccgggatcga ccaggatatt ttcttcggcc
     6181 gtctggtatg agccaccatt gtctccaaga tttattcctc cgcccgattc ggaaggacct
     6241 ccccccttca gccaattccc gacaacccgg atcggacttt ccacccttcc gtgcgaatta
     6301 aaaagattta ttatgtcttc gggggcactt tcgccggata cattttcgca cacattataa
     6361 ctgatgctgt taccggcacc cgatacactg ataaactgaa ccatttgtcc ccatgatttt
     6421 tcaccaccac gcgaatatcc aagaatgttt ttcacatcat tgtattcaaa cctgatattg
     6481 cctgtacagg tggacgcaat catgcctgat gctacatttt cgattacaca gttagtaatg
     6541 gtaatatcag aacaacggta taaataaatt cccaaattgg atattgccgg tcccagttta
     6601 caatttctga tggtaatatg gctacatcca taaagagcaa tacagggatc tttaacatca
     6661 actatttcaa cgccttcaat tataaggttt ttggtatcca cataccttaa ggagggagat
     6721 tttttataca atcctgtata ggctgatccc tgtccggcta caaaac
//
LOCUS       DAOPYB010000167         6756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_13771_length_6756_cov_8.180869, whole genome shotgun sequence.
ACCESSION   DAOPYB010000167 DAOPYB010000000
VERSION     DAOPYB010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13771_length_6756_cov_8.180869"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PKH58_10835"
     CDS             complement(<1..804)
                     /locus_tag="PKH58_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX89570.1"
                     /translation="MKKIFSIILTTCVLVLTGCSDEFFNINQSPNSAVEENMTPSLIL
                     PRALHRTASESAINNDEYLRWMGYWARCSGNYGPNVDEEAYQLSSSFRRTSYLNWYNI
                     LKDFDVIEKNARIRKETAYLGIAKIMKTIGFMQLVDQYNNVPYSKAFDLGSNMLTPYD
                     KGADIYKDMILQLDSADALLKAADVNANLDIKTADIMFKGNLAKWRKFGNTQRLRLIL
                     HQSELLGNAGLKTEIDKIVANGGGFLGAGETAEVQPTYAKDKDKQNPYWN"
     gene            complement(838..3954)
                     /locus_tag="PKH58_10840"
     CDS             complement(838..3954)
                     /locus_tag="PKH58_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX89571.1"
                     /translation="MRKLKFLLTCLLMVSISLVSAQTKTASGTVTSADNGEPVIGASV
                     LIKGTSQGTITNVNGKFTLTVPANATLVISSIGLISQEAAPGTNMEIVLLTDTKTLDE
                     VVVTALGIQRQKRDLGYSTANINSEELTQAKSVSVATGLQGKVAGLNIASLNNGVFED
                     VKINLRGIRSLTGNNNPMLLLDGVPVGLGLLNTINPNDVESVNVLKGTSAAAIYGPDA
                     RNGVIVVTTKAGSKSDKPEITISNSTQFNSVSFFPKFQTSFGSGGYGSYIPYENWSWG
                     PAFDGSEVELGEVLPDGKSVQKVTYSPIKNNRQNFFNTGVTMQNDISYSAKNFYLSIQ
                     DANIKGVVPDDVNRRTGLRMNASQTYKKFTASFNTNYIQSNYNVFDNVAMGDYYSANN
                     VGLNDGLMNLIFSTQAHVPITTYKDFVNNPFAQYNNYYNRYGINPYIALDTWRRKGKD
                     QNLVSSIDLKLAATDWLDLNYRAAMTYQSIVAQSNQKRLVVTPYGKARGIDAVPQAVS
                     NGSYVGNRLSSEFFANFHKTFGDFKVNALAGTYVRDVLSRSTGVNSSNLIVEGLFNVS
                     ARPGELGGYSTESHTRLFSLYGNLNLNYKNWANLEITGRNDKTSVLDPTNNSFFYPGV
                     AGSLVLTDAIPALKSDVMNFLKLRASWNKTGNADISPYLLSATFSQGDGFPYNGLPGY
                     SADDTSYDRYLKPEFINSKEAGFEASFLKNRITIDASYYDQDNTNQIVSIRVPRSTGY
                     TFAFVNAASFRNYGFETQLKVTPLVNFGDVNVNINANYSYNNSEVLSIYQDLQELSIG
                     GYVMAGNQAVVGQPAFIFNASDYQRDDKGRVVVDRLTGYPIVDPVNKKFGRTMPMHII
                     GLNPSISWKGLTVSALFEYKGGHYAFNMIGNEMAWTGVSEITGVNNRERFVIPNSVYE
                     DPKNPGKYIENTNITVSDVQDFFTGDSYRTVASNFITSAASWRFREFSVTYDLPKSLL
                     KGQNLIQGVTVGVNGRNIALWLPKSNVYSDPDFKDLDSFGGNIAGIANATVNPPVRTL
                     GGTISVKF"
     gene            complement(4651..4983)
                     /locus_tag="PKH58_10845"
     CDS             complement(4651..4983)
                     /locus_tag="PKH58_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HNX89572.1"
                     /translation="MGKFIVSLRKDGEFQFNLKAGNGQVILSSQGYATKPSCLNGIES
                     VQKNSQDDSKFERKESTNGKFFFNLKATNGQIIGNSEMYESVAACENGIESVKKNAPD
                     ATVEDQTV"
     gene            complement(5245..5721)
                     /locus_tag="PKH58_10850"
     CDS             complement(5245..5721)
                     /locus_tag="PKH58_10850"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNX89573.1"
                     /translation="MKNFRFVSLFVIAIITSVFVASCKPKDADIKKAVETAIAAIPNT
                     PGITVTVDKQVVTLTGEVIDAETNIAVYEMAASVKNVKSVVNNMTVAAPPTNPEDVQL
                     TAALKDALKDYPTVSYKVMEKVISLTGEIKKSDLPKLLQKVSALKPVKIDNQLIIE"
     gene            6211..6588
                     /locus_tag="PKH58_10855"
     CDS             6211..6588
                     /locus_tag="PKH58_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNX89574.1"
                     /translation="MKKIINTLKAPAAIGPYSQAVEVNGTLYISGQLPINPEAGIIDK
                     QDITSQTEQVFKNIAAILMEAGYEFSDVVKSTVFLSDIQNFAGMNEVYKKYYTSACPA
                     RSAFAVKDLPLGALVEIETIAAR"
BASE COUNT         1982 a         1246 c         1303 g         2225 t
ORIGIN      
        1 gttccagtaa gggttctgtt tatctttatc ttttgcatag gtaggctgaa cttcggctgt
       61 ttcacctgct ccaagaaaac caccgccgtt ggcaactatt ttgtcaattt cagttttaag
      121 accggcatta cccagcagtt cggactggtg taaaatcaga cgaagacgtt gagtattacc
      181 aaatttacgc catttagcaa gattaccttt aaacatgatg tcagccgttt tgatatcgag
      241 gttggcattc acatcagcag ctttaagcag agcatcagca gagtccagtt ggagaatcat
      301 atctttataa atgtcagcac ctttatcata aggggtaagc atattgctac ccaaatcgaa
      361 tgcttttgaa taaggaacgt tgttgtattg gtcaaccaat tgcatgaaac caatggtttt
      421 catgatttta gctataccaa ggtaagcggt ttcctttctg atgcgtgcat ttttttcgat
      481 tacatcaaaa tctttcagaa tgttatacca gttcaggtag cttgtacgtc tgaatgaaga
      541 tgaaagctga taagcctctt cgtcaacatt tggtccatag tttccggagc aacgtgccca
      601 gtaacccatc catcttaagt attcgtcgtt gttgatagcc gattccgaag ccgttctgtg
      661 aagagcacgg ggtaaaatca aactaggggt catgttttct tcaactgcac tgtttggact
      721 ctgattgatg ttgaaaaatt cgtcgctaca tccagtcaga accagtacgc atgttgtaag
      781 tatgattgaa aatatctttt tcatatttat atcttttata gttagttatg ttatcaatta
      841 aaatttaacc gatatggttc caccaagcgt acgtaccggt ggattaacag tagcattagc
      901 aataccagcg atgttaccac caaagctgtc aagatcctta aagtctgggt cgctgtacac
      961 attcgatttt ggcaaccata aggcgatgtt acgtccgttt acacctactg taactccctg
     1021 gataagattc tgacccttta acagagattt gggtaagtcg taagttacag agaattctct
     1081 gaaacgccat gaagctgcgc ttgtgataaa gttggatgcc actgtgcgat agctgtcacc
     1141 tgtgaagaag tcctgtacat cggaaacggt tatatttgta ttttcaatat atttaccggg
     1201 atttttagga tcttcataaa ctgagttcgg tatcacgaaa cgttcacggt tattaacgcc
     1261 tgtaatttca gatacaccgg tccaggccat ttcattacca atcatattaa aggcataatg
     1321 tccgcctttg tattcgaaca acgcagatac agtcagacct ttccagctga tggaaggatt
     1381 cagacctatg atgtgcatcg gcatggtgcg accgaatttt ttatttaccg gatcaacgat
     1441 agggtaacct gtcagacggt caacgacaac gcgtccttta tcgtcgcgtt ggtagtcaga
     1501 tgcattgaaa ataaatgcag gttgacctac tacagcctgg ttgcctgcca ttacatatcc
     1561 accgatggaa agttcctgaa gatcctgata gatgctcagt acttcgctgt tgttgtatga
     1621 atagttggca ttgatgttga cgtttacatc accaaagttt acaagcggag taactttcag
     1681 ctgagtttcg aaaccgtagt ttctgaaaga tgcagcattc acgaaagcaa aagtataacc
     1741 ggttgaacgt ggaacgcgaa tgcttacaat ctggttggta ttatcctgat catagtacga
     1801 tgcgtcaatg gtaattctgt ttttcaggaa gctggcttca aaacctgctt ctttactgtt
     1861 gataaactca ggtttcaggt aacggtcgta tgaagtatcg tcagctgagt atcccggaag
     1921 tccgttatat gggaaaccat caccctgtga gaaagttgca ctcaacagat aaggactgat
     1981 atctgcatta cctgttttgt tccatgaagc gcgaagtttc aggaagttca ttacgtctga
     2041 tttgagagca ggaatagcat cggtaagtac caatgacccg gcaacacccg gatagaagaa
     2101 gctgttgttg gtcggatcca gaacggatgt tttgtcgtta cgtcctgtaa tttccaggtt
     2161 agcccaattt ttatagttca ggtttaagtt accgtagagt gagaataaac gggtgtgaga
     2221 ttcggttgaa taaccaccta attcacccgg acgggcagaa acattgaata aaccttcaac
     2281 aataaggttg ctgctgttaa cacctgtact gcgtgataat acgtcacgaa cataagtacc
     2341 tgcaagtgca tttaccttga aatcgccgaa tgttttgtgg aaattggcaa aaaattctga
     2401 tgaaagacgg ttgccaacat acgaaccgtt gcttactgct tgtggaacag catcaatacc
     2461 tcttgcttta ccgtaagggg ttaccacaag acgtttctgg ttgctttgag ctacaatgct
     2521 ctgataggtc atagcggcac ggtagttcaa atcaagccag tctgttgcag caagtttgag
     2581 gtctatactt gaaaccaaat tctgatcttt accttttctt ctccaggtgt caagtgcaat
     2641 gtaaggattg ataccgtacc ggttgtagta gttgttgtac tgagcaaacg ggttgtttac
     2701 gaaatcttta taagtagtga taggcacgtg agcctgagtg ctgaaaatga ggttcatcaa
     2761 accgtcgttc aaaccaacgt tattggctga atagtagtca cccatagcca cattgtcaaa
     2821 tacgttgtag tttgactgaa tgtagtttgt attgaaagaa gctgtaaatt tcttgtatgt
     2881 ctgtgaagca ttcattctta aaccggttct tctgttaaca tcatccggaa ccaccccttt
     2941 gatgttagcg tcctgaatac tcaggtagaa gtttttggca gaataagaaa tgtcgttctg
     3001 catagttacg ccagtgttga agaagttctg gcggttgttt ttgataggcg aataagttac
     3061 tttctgaacg gattttccgt caggaagcac ttcgccgagt tccacttctg aaccatcgaa
     3121 agcagggccc catgaccagt tttcataagg tatataactt ccgtaaccac ccgaaccgaa
     3181 agaggtctgg aatttaggga agaaagatac gctattaaat tgtgtagagt tgctgattgt
     3241 tatttccggt ttgtcgcttt tcgaaccggc ttttgttgta acaacaatca caccgttgcg
     3301 ggcatcgggt ccgtatatag cagctgcaga agtacctttc agtacgttta cactttccac
     3361 gtcatttggg ttaatcgtat tcagaagtcc aagtcctaca ggtactccat cgagcaaaag
     3421 catcgggttg ttattacctg taagtgaacg gataccgcga aggttgatct ttacatcttc
     3481 gaatacacca ttatttaaag aagcgatatt caaaccggct actttaccct gcaaccctgt
     3541 tgcaacgcta actgatttag cctgtgttaa ttcttcgctg ttaatgtttg cggtagaata
     3601 acccagatct cttttctgtc tttgaatacc gagagctgta actaccactt catccagagt
     3661 ttttgtgtct gtgagcaaga ctatttccat attagttccc ggagctgctt cctgtgaaat
     3721 taatccaatg gaagaaataa ctaatgtggc atttgcagga acagttaagg taaatttacc
     3781 gtttacattg gtaattgtac cctgtgaagt ccctttaatt aaaacgctgg caccgattac
     3841 cggttctccg ttatctgctg aagtgacagt accggaagct gttttcgttt gagcgctaac
     3901 caaactaata cttaccataa gcaggcaagt taacaagaat tttagttttc tcataaattt
     3961 aacattttgt ttattaatct ttttaattgt gtttactaaa ataaacctta aaacctaatt
     4021 tgcaaaaata aacgattgtt atttaaattc caaatttatt ttcaacgaaa tgtactttaa
     4081 atcgaagatt ttattgcaaa atgttatttt ttgtccggtt acaatttatt ctgttactat
     4141 aaaaatgtta ctcaaatttt taataatcga cgtctaaatt acttattgta agcattttgt
     4201 tttttcaacc ataaaatcag tttccgaata aaaattgaca ttttaatgtt tgtatgtaaa
     4261 gcgaataaat tttcaaaaaa ccataaattt aacactttat ttttgaaaaa tcgttttttt
     4321 tctatgcctt gtttctcgaa aatgtaataa aattggtttt ttaatccttt ctattattta
     4381 gttttcgcac tataatgcaa ttttaaagag ctggaaaggg caatatttgt tcatttgcaa
     4441 gtgtaagtgt ttttattcat cagaatgaat cgtaaagtta aaatgctgac aatttgacag
     4501 tgctacgaaa agcactgaat tacaggtata gaaagaatat ttgtgattac cgaaaacttt
     4561 tcgtgaaatg aaggagaagt tactgaaaaa agaagctgtc attttttttg acagcctcat
     4621 cgttttaggg ttggaaatac atactcaagt ttacacggtc tgatcttcaa ccgtagcatc
     4681 cggtgcattt ttcttaaccg attcaatgcc attctcgcag gcagcaacac tttcgtacat
     4741 ttcactattc ccaataattt gcccgttggt tgctttgaga ttgaaaaaga attttccatt
     4801 agtcgattct ttccgttcaa atttcgaatc gtcctgagag tttttctgaa cagactcaat
     4861 gccattcaag caacttggtt tggtggcata gccctggctg cttaaaatta cctggccgtt
     4921 tccggctttc agattaaact gaaattctcc gtcttttcgg agagaaacaa taaattttcc
     4981 cataaagatt tttttaatta gagttataac tgtttttttt gaactggttc aaggtgttgt
     5041 gtataaaatc agctttttcg acgtcatagt tgtatgtcag ctactattga ataacggtat
     5101 ttcctacaat cagctgatcg gttatcgtct cttttcggtt atttggtttg ccggaaagta
     5161 ttacaactcc ggtttgttcg aaaaactgcc agacttcgct gcgtcaattc caaagataat
     5221 ttttggtaat tgcccggatg gtgattattc aatgattaat tgattgtcga tttttaccgg
     5281 tttgagcgcc gaaacttttt gcagtaactt tggtaaatca gattttttta tttcaccggt
     5341 gagagagatt actttttcca taaccttata tgatacagtc ggataatctt tcagggcatc
     5401 tttcagtgcg gctgtaagtt ggacatcctc cgggttagtc ggcggtgctg caacagtcat
     5461 gttgttaaca accgatttca cattttttac agatgcagcc atttcataca cagctatgtt
     5521 tgtttctgca tctattactt caccggtaag agttacaacc tgcttatcaa cagtaactgt
     5581 gattccgggt gtattcggaa tagctgctat agcagtctca acggcttttt taatgtctgc
     5641 gtcttttggt ttgcaggatg caacaaaaac ggaggttata atagcaataa caaacaagga
     5701 aacaaaacga aagtttttca tgtttttttg ttttagtgtt aattattaca ttaatacaca
     5761 tgattatata tgattatggt atcacatata attatagtta atgtttttca ataagtaatt
     5821 tgaaatgtaa atataattgt gagttttgaa ttatccaaat attttatcat ttatttatta
     5881 gtttggcaaa ataaattgta ctttaaaaat ttaattgcag aaaccaggcg gaaaaattgc
     5941 tattgcacaa aaattttaag aatactgttt ttcacaagaa attctctcag ggtaaccctt
     6001 gtgtaataat agtttaattc acctacagaa tacaaaaaaa caaaatgttt tgctagtttt
     6061 ttcggtatga ttaagcgaac agtttaatta cttgtttgat gactaagttt ataaacacta
     6121 aattttccgt aaaaattcag tgtttaaaaa tctttaatta attttgtcgc aatctattca
     6181 atgatttagg aacttaatct acaaacacaa atgaaaaaaa ttattaatac cctcaaagca
     6241 cccgctgcga ttggtcctta tagtcaggct gttgaagtta acggcacact ttatatatct
     6301 ggtcaactac ctattaatcc tgaagcggga attattgata aacaagatat tacatcacaa
     6361 actgaacagg ttttcaaaaa catagctgcc atactcatgg aagccggtta tgaattttcg
     6421 gatgtggtaa aatcaaccgt ttttctttca gatattcaga attttgccgg tatgaacgaa
     6481 gtgtataaaa aatactatac ttctgcatgt cccgcccgtt cggcttttgc tgttaaagat
     6541 ctgccactgg gagcattagt ggaaatagaa acaatagctg cccgatagat tattttgttt
     6601 tgagttccgg ttaaaacata atcggaaatc aaagctgtga attttctgaa aatattaaat
     6661 gacatcgccc gacttaaatg aaactaagtt atataacgct cgattaaata aatataaaag
     6721 gatggtcatt tcagatcatc cttttatatt tattac
//
LOCUS       DAOPYB010000168         6638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_14121_length_6638_cov_10.117727, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000168 DAOPYB010000000
VERSION     DAOPYB010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14121_length_6638_cov_10.117727"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1799)
                     /locus_tag="PKH58_10860"
     CDS             complement(114..1799)
                     /locus_tag="PKH58_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89575.1"
                     /translation="MKNTSKTVFCKCQGRIGLILIILLSMTGITAFGKNCERMVYVYD
                     IAVTVPNPSPELYQASDGCVEQLLLNSRASNEFFSLDFQPVRVLKPATEQALIGPGQG
                     PSSNIDYMIWGELKETGTGRYNITLYLVTANTRLQVAKASSTFTNPSEAKFAGMTAAL
                     SLGASGTGSRRLIDVITDYEKKVREDDNRKTICPDMVYLMKEKTIEAKQEEEVLLMYQ
                     LKDTDGKPVKDADVSARCDEGSLDMQETKTDQYGMVRFKHKTPDKNTEYSIRCFAKAI
                     APSEKIIRVDDVIVPVKVKKKITELTGEIEIQEFTKTGNPTLANTEQGKSTFHSVAVS
                     PLDLTIIPERIIQINKSRDVLSRALELDEFSIVDGLTMKDKTGEPVSVRSKSEYNSYL
                     PCEDKLQLDMTEQIEGSSNGFDIRVSIALERGIETGNTTLSSLSPRYCLVITPGSNAR
                     LLFQSSKYRTTATRSGQRRDYPCGELTSFSEEVNPKYELAHGAMRSTIQKNDQEYHEI
                     IVPLSIPDSKALEEYLLNPKGPYSIRVSGKYIKKDYEERETQINATLLMMPKE"
     gene            complement(1921..4359)
                     /locus_tag="PKH58_10865"
     CDS             complement(1921..4359)
                     /locus_tag="PKH58_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNX89576.1"
                     /translation="MKNYYYYLLLILWGVSANASAQDYRLWYNKPAREWTDALPLGNG
                     RLGAMVYGIPAQEHIQLNEQTIWGGSPYRNHNPKALQALSEVRRLVFEGKNQKAQHLI
                     DSTFSTPYKGMPYQTMGDLYLTFPGHEQYSGYCRSLSLDSAISVTTYKVNNITFRRET
                     FSSFADNVIIMRISADKPGSVTFTASYNSPQTIYKERDGNNLVVSGTTTDHEGIPGKV
                     KFENQTLIKADGGKVLVTDSTIQVRGARSAVIYISIATNFRHYDDISGNQSDKASNYL
                     DKALKSAYTKALKNHIASYQKYYRRVALDLGKTDAAQNPTDDRIRAFQQGNDPQLAAI
                     AFQYGRYLLISSSQPGGQPANLQGIWNEKLKAPWDGKYTININLEMNYWPSEVTNLSE
                     MSEPLFQLIKDISETGQETAKSMYGVNGWVAHHNTDIWRCTGVIDNAYYGAWPNGGAW
                     LCQHIWQHYLFTGDKKFLARYFPVLKGSADFFLDFLTEHPSYHWLVTCPSISPENSPV
                     PRGSTITAGCTMDNQIAFEILSNTRAANNLLGGDKTYSEKLQKTIDRLPPLQIGKYNQ
                     LQEWLEDLDDPQSEHRHVSHLFGLYPGVQISPYKNPELFQAAKQSLIFRGDKATGWSI
                     GWKINFWARLLDGNHAYKLISNMLTLVEPDSKDTHTYTNMFDAHPPFQIDGNFGLTAG
                     IAEMLLQSHDDALHLLPALPDVWSAGLVTGLKARGGFEVDMKWTKGKLTEARIRSSAG
                     GNLRIRSYLPLKGDRLKNAEGTNTNPLFRSEPIKQPLYASNGVPPMPELRKVYEYDIQ
                     TKPGAVIKIFAVND"
     gene            complement(4409..4795)
                     /locus_tag="PKH58_10870"
     CDS             complement(4409..4795)
                     /locus_tag="PKH58_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89577.1"
                     /translation="MKEPRRGCPVQIVNSHEKVVNSQTFYNAAANFYGYSTYNSMKNQ
                     RKAIAESLDLLDMIDHQDSPVYLMNLLKAQFPVDNIIIQHHKKHAVVLSKTLNKHGVK
                     NYLFTEQNAGKDMVYPVRQFLVDNLK"
     gene            complement(4842..>6638)
                     /locus_tag="PKH58_10875"
     CDS             complement(4842..>6638)
                     /locus_tag="PKH58_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX89578.1"
                     /translation="DVGDGIDYYFMCGENADAVIAGMRNLTGQAPMFPLWTYGFWQSR
                     ERYKSQDELVDVVKKFRELNVPLDGIIQDWQYWGSNYLWNAMEFLNPGFNNPQKMVND
                     VHGLNAHMIISVWNSFGPMTKQYRELEKINALFNFETWPQSGSEKWPPRMDYPSGVRV
                     YDPYNPTARDIYWNYLNKGLFSLGMDGWWMDSSEPDHFNPKPSDYDTRTFLGSFRKVR
                     NAFPLMTVGGVSQHQRSFTSDKRVFILTRSAFAGQQRYGANTWSGDVAASWEALRNQI
                     SAGLNFSLCGIPYWNSDIGGFFLWNFKNPLTNVDYRELHVRWMQFGTFCPMMRSHGEG
                     VPRELYQFGKKGNPTYDAIEKYINLRYTFLPYIYSASWDVTARQSSMMRALVMDFAGD
                     KQALDINDEYMFGSSLLVNPVTQPMYSKTVMSGGDSVKVEDFSTVKSKDVYLPKGTDW
                     YDFWTGEKFNGGQTIKKEVPLDIIPLFVKAGTILPVGPEVQYATEKKWDNLEIRVYEG
                     ADGEFTLYEDENDNYNYEKGLYSTISFSWNDRNKSLTVGECKGSFPGMLKSRRFNIVI
                     VGKSKGVGAELVENYDKVVSYTGKKLVITPKK"
BASE COUNT         1654 a         1551 c         1432 g         2001 t
ORIGIN      
        1 taactcattt gttgagaatg ttttactgct ttgctggcaa aaatattgat actccgaaat
       61 gcagctgtgg taaagttata cgaacaggtc cttgtaaaag gccagacttt ttttcattct
      121 tttggcatca tcagcagggt agcgttgatt tgagtttctc tctcttcgta atctttttta
      181 atatatttac ctgaaacgcg aatgctgtaa ggacctttcg gattcaacag atattcttca
      241 agtgctttgc tgtctggtat cgagagagga acaataattt catggtattc ctgatcattt
      301 ttttgaattg tacttctcat agcaccatgc gcaagctcgt attttgggtt tacttcctca
      361 gaaaatgaag tcagttcccc gcacggatag tctctcctct ggccgcttct tgtggcagtg
      421 gttctgtatt ttgacgattg gaacagtaac cgcgcattcg atccgggggt gatgacaaga
      481 cagtatctcg ggctgagact ggagagtgtc gtgtttccgg tttcaatacc ccgttcgaga
      541 gcaatgctaa cccggatgtc gaacccgttg ctgctacctt ctatctgttc cgtcatatcc
      601 agttgcagtt tgtcctcaca gggcagataa ctgttgtatt ctgatttgct tcgtacggaa
      661 acaggttctc ctgtcttgtc tttcattgtc agaccatcaa caatgctgaa ctcgtccaat
      721 tccagcgccc tgctcaacac gtccctgctt ttgtttatct gaataatacg ttcggggatg
      781 atcgtcaaat ccaggggcga tactgctacc gaatgaaaag tgcttttacc ctgttcagtg
      841 ttagcaagcg tgggatttcc ggttttggta aactcctgaa tttctatttc tccggttaat
      901 tcggttattt tctttttcac ttttacaggt acaatcacat cgtccaccct gattattttt
      961 tcactgggtg ctatagcttt ggcaaaacaa cgtatgctgt actccgtatt tttatcaggt
     1021 gtcttgtgtt tgaacctgac cattccgtac tgatccgtct ttgtttcctg catatcgaga
     1081 cttccctcgt cgcatcttgc tgaaacatcg gcatctttta ccggtttgcc atcagtatcc
     1141 ttcagctgat acattaacaa tacttcttct tcctgtttgg cttcaattgt tttttccttc
     1201 atcaaataca ccatatccgg acaaatcgtt tttctgttgt catcttccct cactttcttt
     1261 tcgtagtcgg taatgacgtc aatcaaccgg cgtgaacctg ttccggatgc acccagactt
     1321 aatgcagctg tcatacctgc aaatttggct tcagaaggat ttgtaaacgt tgaacttgct
     1381 tttgcgacct gaagtctggt gttagccgta accaggtaga gagtaatatt atatctgcct
     1441 gtacctgttt ctttcagttc accccatatc atatagtcga tgttggagga tgggccctga
     1501 ccgggaccaa tcagcgcctg ttctgttgcc ggtttcagga cgcgtacagg ctgaaagtca
     1561 aggctgaaaa attcatttga cgctctcgaa ttcagaagca gctgttctac acaaccgtca
     1621 ctggcctgat aaagctctgg cgaagggttt ggtacagtta cagcaatatc atacacatat
     1681 accatccgct cacaattttt gccaaaagcg gttatacccg tcatactgag taaaataatc
     1741 aggatcagac cgattcgtcc ctgacacttg cagaatactg tttttgatgt gtttttcatg
     1801 tttatttttt attttatatt gtcagatagc ctgtcccgat ttatcgggag aatatgccat
     1861 tgatcatttc cttttgtttg agaattttca caaaggtgtg ttatatgctc ttaagtctga
     1921 ctaatcgttt accgcgaaaa ttttaatcac tgcgcccggt ttcgtttgta tgtcatattc
     1981 ataaacctta cgcaactccg gcataggtgg aacaccgtta ctggcataca aaggctgttt
     2041 aattggttcg ctgcgaaaca acggattggt gttcgttcct tctgcatttt tcaatctgtc
     2101 gcctttcaac ggaagatacg agcgaatacg caaatttccg cctgctgatg aacgtatccg
     2161 ggcttcagtc agttttcctt tagtccattt catatcaact tcgaaacctc cgcgggcttt
     2221 caatcccgtg actaaccctg cagaccacac atccggaaga gcaggcaaga gatgcaatgc
     2281 atcgtcgtgg ctttgcagga gcatttcggc aattccggca gtcagtccga aatttccgtc
     2341 aatctgaaag ggcggatgag catcaaacat gttggtatag gtatgcgtgt ccttcgagtc
     2401 gggttcaacc agggttaaca tattggatat tagtttgtac gcatgattgc catcgagcaa
     2461 gcgtgcccag aaatttatct tccagccaat agaccaaccg gtagccttat caccccggaa
     2521 aataagcgat tgtttggcag cctgaaaaag ttcagggttc ttatacggcg aaatttgcac
     2581 acccggatac aaaccaaaca agtgagacac atgcctgtgc tccgattgcg ggtcatccaa
     2641 atcctcgagc cattcctgca attggttgta tttaccaatc tgcaagggtg gcagacggtc
     2701 aattgttttt tgcagctttt cagagtatgt tttgtcgcct cccagaagat tattcgcagc
     2761 ccgtgtgttc gatagtattt caaaggctat ctgattatcc atggtgcatc cggctgttat
     2821 tgtggagcct ctgggcaccg gactgttctc cggcgaaatg gaaggacagg ttacgagcca
     2881 gtgatacgac ggatgttctg ttagaaaatc gaggaagaaa tcggctgaac ctttcagtac
     2941 ggggaaatag cgggccagaa actttttgtc gccggtgaac aggtaatgtt gccatatatg
     3001 ctggcacaac caggcacctc cgtttggcca ggctccgtaa tatgcattgt caatcactcc
     3061 tgtacaacgc cagatatcgg tattgtgatg agctacccat ccgttgactc cgtacatcga
     3121 ttttgctgtt tcctgtcctg tttccgaaat gtctttgatc agctgaaaca acggttcgct
     3181 catttcggac aggttggtca cctccgatgg ccagtaattc atctcgagat taatatttat
     3241 cgtatatttt ccatcccacg gggctttaag cttctcgttc cagataccct gcaggtttgc
     3301 cggttgtcca ccgggctgag aacttgagat gagcaggtat cgtccgtact gaaaagcaat
     3361 ggctgcaagt tgcgggtcgt tgccttgctg aaaagccctg atgcggtcat cggttgggtt
     3421 ttgcgcagca tcggttttgc ccaggtcgag cgcaactcgt ctgtaatatt tttggtacga
     3481 tgcaatatga tttttcagcg ctttggtata ggctgatttt aatgctttgt ccagataatt
     3541 actggcctta tcagactgat tgccgctgat gtcgtcgtaa tgtctgaaat tggtagctat
     3601 cgaaatatag atgacagccg aacgggcacc ccggacctgt atagtcgaat cggtcaccag
     3661 cactttaccg ccgtcggctt ttatcagcgt ctgattttcg aatttcactt ttccgggaat
     3721 tccctcgtgg tcggtagttg tgccgcttac aacaagattg tttccgtctc tctctttgta
     3781 aatagtctgc ggagaattgt aagacgcggt aaatgttacc gagcctggtt tgtcggctga
     3841 aatccgcatg atgattacgt tatcggcaaa cgacgagaaa gtttctcgcc tgaaggtgat
     3901 attatttacc ttgtacgtcg ttaccgaaat ggcgctgtcg agactcaggc tgcggcaata
     3961 accggaatat tgttcgtgtc ccgggaaagt cagatataag tcgcccatgg tctgatacgg
     4021 cataccctta taaggagtgg aaaaggtcga atcaatcaaa tgctgagctt tctgattttt
     4081 cccttcaaaa acaagccgtc gtacttcgga caaagcctgc aatgccttcg gattatgatt
     4141 ccgatacggg ctgccacccc aaattgtttg ttcgttcaac tgaatgtgtt cctgagccgg
     4201 aatgccatac accatcgctc caagtcgtcc gttgcctaaa ggaagcgcat cagtccattc
     4261 ccgcgcaggt ttattgtacc ataacctgta atcctgagct gacgcattgg ctgaaacgcc
     4321 ccaaagtatc agaagtaaat aataatagta gttcttcatt gtatgtattt tgtagatatc
     4381 agttgtatgc agcgcgtctg caacgacatt atttcaaatt gtcaacaaga aattgtctga
     4441 ccggatatac catatctttc ccggcattct gttcagtaaa taaatagttc ttaacgccgt
     4501 gtttgttcag tgtttttgaa agaaccacag catgtttttt atgatgctgg atgataatat
     4561 tatcaacagg aaactgagct ttcagcaggt tcatcagata cacgggcgaa tcctgatgat
     4621 caatcatatc gagcaaatcc aggctttcag ctattgcttt tctctgattt ttcatgctat
     4681 tgtatgttga ataaccatag aaattggcag cggcgttata aaaagtctgg ctatttacta
     4741 ctttttcatg gctatttact atctgaacag ggcaacctct acgaggttct ttcatcggtg
     4801 catcttcatc cccccggata aatcctgagg ctatgcacag tttacttttt cggagtaatt
     4861 accagttttt tacctgtgta gctaaccacc ttatcgtaat tttcaactaa ttccgcgcca
     4921 actcctttgc tttttccaac aatcacgatg ttaaacctgc gtgatttaag cattccgggg
     4981 aaagagcctt tacattcacc aacggttaat gacttgttcc tgtcattcca ggaaaaagag
     5041 attgtagaat aaaggccttt ttcatagttg taattgtcgt tttcatcttc gtacaatgta
     5101 aattcgccgt cagcaccttc ataaacgcgg atttccagat tatcccattt cttttcggtg
     5161 gcatactgaa cttcaggtcc aaccggcaaa atggttccag ccttaacaaa caacggaatg
     5221 atgtccaacg gaacctcttt cttaatggtt tgtccgccat tgaatttctc accggtccag
     5281 aagtcatacc agtcggtgcc tttgggcagg taaacatctt tggacttaac tgtactgaaa
     5341 tcctctactt ttacggaatc gccacctgac ataactgttt tggaatacat gggctgtgta
     5401 accggattga ccagcaacga actgccaaac atatattcat cgttgatatc cagcgcctgt
     5461 ttgtcaccgg cgaaatccat aaccagcgcc cgcatcatgc tcgactgccg ggctgttaca
     5521 tcccatgaag ctgaataaat atagggcaag aaggtgtatc gaaggttgat atatttttca
     5581 atcgcgtcgt aggttggatt tcccttttta ccaaactgat aaagctccct tggcacccct
     5641 tcaccatgcg aacgcatcat gggacagaaa gtaccaaact gcatccatct gacatgcaac
     5701 tcgcggtaat caacatttgt aagcgggttt ttaaaattcc acagaaagaa acctccaata
     5761 tcgctgttcc agtatggaat gccacacagt gagaagttca atcccgccga gatctgattg
     5821 cgcaaagcct cccacgaagc tgctacatcg cccgaccagg tgttagcacc gtagcgctgc
     5881 tgaccggcaa aagccgaacg ggtaagaatg aatacacgct tgtcggatgt gaacgagcgc
     5941 tggtgttgtg aaactccgcc gacagtcatt aacggaaagg cattgcgcac tttccggaat
     6001 gaacccagga atgtgcgtgt gtcataatca gagggtttcg ggttaaagtg gtccggttcc
     6061 gaagaatcca tccaccatcc atccattccc agtgagaata gtcccttgtt taaataattc
     6121 cagtaaatat cacgcgcagt aggattgtaa gggtcgtaca cgcgaactcc cgatggataa
     6181 tccatgcgtg gcggccattt ttcagaaccc gattgcggcc aggtttcaaa attgaagagc
     6241 gcgttgattt tttcaagttc cctgtactgc tttgtcatcg gaccaaatga attccagacg
     6301 gagataatca tgtgcgcatt taaaccgtga acatcattta ccattttctg aggattgttg
     6361 aatccgggat taaggaattc cattgcattc cagagataat tgctgcccca gtactgccag
     6421 tcctgaataa taccatccag cggaacattt aattcgcgga atttctttac cacatcaacc
     6481 agttcgtcct gacttttgta gcgttcccta ctttgccaga aaccataagt ccacaacggg
     6541 aacatagggg cctgtccggt gaggttacgc atgccggcaa tgacagcatc ggcattttcg
     6601 ccgcacataa aataataatc aataccatcg ccaacatc
//
LOCUS       DAOPYB010000169         6615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_14183_length_6615_cov_10.306098, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000169 DAOPYB010000000
VERSION     DAOPYB010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14183_length_6615_cov_10.306098"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKH58_10880"
     CDS             <1..878
                     /locus_tag="PKH58_10880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNX89579.1"
                     /translation="KNNMLKETFRNSGLFGKLLQLFFISFFFGIAGIGLVSVIFGATT
                     NIYDQKIMQLILTLFTFLLAPIAVGYIWYEKPSDAYSLNRKPPVKMIFLVILLMFAIT
                     PLINLLSYVNEQMVLPGFMKNIEVYFKEMEDAAAQLTEKMLVMNTTSDLIVNLIVMAV
                     APALCEELYFRGTLLNIFSEGRNRHAAIWIVAIIFSLIHFQMYGFIPRMILGALLGYL
                     MMWSGSIWVPVIAHFTNNAMAVITSGFGQTNEQLKKLEDIGKAETFWYGIVSLLITIL
                     IIREIYKIRNKETAG"
     gene            complement(893..1291)
                     /locus_tag="PKH58_10885"
     CDS             complement(893..1291)
                     /locus_tag="PKH58_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNX89580.1"
                     /translation="MIALIFVLVLSALILFIATFLARKNKKEEVEIVINEDRECCGAH
                     EVCERDNLQIKDIKIEYFEDEELDELCGLSPEKFTDKQNEALAYVLYSLKESEIAPWL
                     RSLQLRGIQLPSSLREQALMIVGERRDKIG"
     gene            1466..4942
                     /locus_tag="PKH58_10890"
     CDS             1466..4942
                     /locus_tag="PKH58_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX89581.1"
                     /translation="MKRKIIHIACLLLAVMLINACSTKKNTWSTRSYHSLTTRYNIAF
                     NGSESYKDGLENISKANKDDYSQVIPMYPVSNKASATAAVSNMDRTIEKCRKAIKLHS
                     IRKKPQKNYKKANDPKYLSFYNQNEYNPALRDAWMMLGKAEFHKGDFLGSVGTFSYIG
                     RYYASVPEMVVQSQIWTARAYTEMGWIYEAEDLLQKVNQDQITGKNTGLYAAVNANLM
                     IKKGQYKEAIPFLKLAIEHEKNKKNRTRFNFVMAQLQTIAGDKKAASDYYTEVIKSNP
                     GYEMTFNARINRSQLLAVNLRSVEKELKKMARNRNNRDYLDQIYAATGNVYLQSKDTA
                     NAIENYNLALEKSKRNGIDKAVAALKLGDLYYNRKDYVKSQPCYDEASKIFTNDYTDY
                     ARISKRAEVLGELVKEHEIIVLQDSLQALALMSEDKRKEAINRVIEKVKKEEKEAAEK
                     EKNPQGQGGMYDTEDNFTMPIGTPIGGGNNRNWYFYNPVTISSGKADFRKKWGNRKLE
                     DNWRRNNKSAALFADNAAGATATAVSTLTAGNDSTKLPKIKATSDKTSVDYYLNQIPF
                     TTEQRKKSDAEVADAMFNKGLIFKDKLEDFQMAYNTFDEFERRFGKDDRILETLFQRY
                     LMASREKNELLAEQYRNRILSNYPESKYAQLIKDPNYILKQGMILAEQDSLYSQTYTA
                     YTRSDFPAVFRNTENFRKKYPVSSLLPKFEFLNTLSIGKTATSVDFEKSLSKLVENYP
                     ESDVSAMSKDILALMKQGNLAQKGTTHGELLSKREAADKTTEKQDDMSFTAEKQGKHR
                     LLLLTDATTEGVNKLLYNLAIFNFSRFMIKDFDFIVGQADATHQSISVTNLESYDEVI
                     WYQKTLKTDLALSALMDSLKVQQVAISEDNFGKIKSIFTLDEYLAFEKANLIKDKPAN
                     IASNTTTATVKKEELKKTLPEINKTVPVKETVVASVTKPTTEQKNISPAETKTTPIVP
                     EQKAAAQQTKQAETTKTETAKTESTGKTPVTDSKQQTTPENVKATTEKMPEKPAVVEE
                     KVEWYKNLFAIRLNAEHFVAFYLPPGKFDFVKIQKALDAYNAANYNMMNLKVSLEDSG
                     KDKFIIVGAFPDANVAKSYFLRMLKDPAIIEAFKGVNRRNLVGTKENLNIMVKKDALN
                     TYFEFMREYYLK"
     gene            5051..6598
                     /locus_tag="PKH58_10895"
     CDS             5051..6598
                     /locus_tag="PKH58_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HNX89582.1"
                     /translation="MKKDRILWADDEMDLLKPYLLFLEEKGYEVVAVTNGKDAIELCQ
                     NESFDIIFLDENMPGLSGLQTLAQIKTIDPNVPMVMITKSEEEDIMNMAIGNKIADYL
                     TKPVNPSQILLTLKKNIHKKEIVSEHATSSYQQEFRNIGMQINDSFTYQDWVDVYKKL
                     VYWELELAETENGMDEMLKMQKNEANSTFNKFVKKNYLDWMTNPDQRPLMSTDLFKTK
                     VFPLIDKGEKVFFIVVDNFRYDQWIIIRELLSEFYTFNEEETYCSILPTATQYARNAI
                     FAGLMPLQIQQMYPHLWIEEEDEEGKNINEEALLKTQLDRFRKKYSFSYHKVFESGFC
                     EKVINQLPSLENNQLNVVVLNFIDMLSHARTESKMMRELANDEEAYRSLTLSWFRHSA
                     TYDLFKAIAQRGYKVVFTTDHGTIQVNNAIKVVGDKNVNTNLRYKVGKNLGYNKKEVF
                     EITQPAKAGLPSPNVSSAYIFAGNDDFFAYPNNFNYYVTYYKNTFQHGGISLEEMIIP
                     IVTMEPK"
BASE COUNT         2230 a         1389 c         1330 g         1666 t
ORIGIN      
        1 aaaaaaataa tatgcttaaa gaaactttca gaaattcagg actgtttggc aaactgttac
       61 agcttttttt tatcagtttt ttcttcggaa tagccggtat cggtctggtg tcagtaatct
      121 tcggagctac aaccaatatt tacgaccaga aaatcatgca gcttatttta acccttttta
      181 cttttctgct tgcaccaata gctgtgggat atatctggta cgaaaaaccc tcagacgctt
      241 attcgctgaa ccgaaaacca cctgtaaaaa tgattttttt ggtcattttg ctgatgtttg
      301 ccattacacc gctaattaac ctgctttcgt acgtcaatga gcagatggtc ctgcccggtt
      361 ttatgaagaa tattgaggta tatttcaaag aaatggaaga tgccgcggct cagctcaccg
      421 aaaaaatgct ggtgatgaat acaacctccg atttaattgt aaatctgata gttatggctg
      481 ttgcaccggc actgtgtgag gaattgtatt ttaggggaac tttactgaat atattttcgg
      541 agggaagaaa ccggcatgca gctatatgga ttgttgccat aatattcagt ctgatccatt
      601 ttcaaatgta cggatttatt ccgcggatga ttctgggagc tttactgggt tatctgatga
      661 tgtggagcgg aagtatctgg gtgcctgtta ttgctcattt caccaataat gcaatggcgg
      721 tgatcacctc cggtttcggt cagacaaacg agcaactgaa aaaactggaa gatatcggaa
      781 aagctgagac attctggtac ggaatcgtga gtttacttat aacaatcctg ataatcagag
      841 aaatatataa aatcaggaat aaagaaactg caggataatc cgaacattca tttcatccta
      901 tcttatctct cctctctccc actatcatca acgcctgttc gcgtaaggat gaagggagct
      961 gaataccgcg cagctgcaaa cttctcagcc agggagcaat ttcagattct ttcagcgaat
     1021 atagcacata tgcaagcgct tcgttctgct tgtctgtaaa tttttcgggc gataatccgc
     1081 agagttcatc cagttcctca tcctcgaaat attctatttt tatatcttta atctgcaggt
     1141 tgtcacgctc acaaacctca tgagcgccgc aacactcccg atcttcattg ataacaattt
     1201 cgacctcttc ttttttgttt ttacgggcta aaaaggtagc gatgaataaa atcagtgcgg
     1261 acaaaaccaa cacgaaaatc aatgcaatca tttgaatata aagtttcagg atttcggtaa
     1321 tgaaacaact atttgcagac aaagttagat attgttgact gaaaaattgt atttttgtag
     1381 gttaaaactt tatttatcac acaataaaac tcatacaaaa tggttttttg ttatgttgaa
     1441 ttatagccgt aaacattaaa ttatcttgaa acgtaaaatc atacatatag cctgtctgct
     1501 gctggcagtg atgctgataa atgcctgttc gaccaaaaaa aacacctggt cgacaagaag
     1561 ttatcattct ctcacaacgc gttacaatat tgcattcaat ggttccgaga gttacaaaga
     1621 tggacttgaa aatatcagta aggcaaacaa ggacgattac tcgcaggtga tacccatgta
     1681 tcctgtcagc aataaagcca gtgccacagc tgccgtttca aacatggatc gaaccattga
     1741 gaaatgccgg aaagccataa aacttcactc catccgcaaa aaacctcaga aaaattacaa
     1801 aaaagccaac gaccccaaat acctttcttt ctacaaccag aatgagtaca atcccgcact
     1861 gcgcgatgcc tggatgatgc tcggcaaagc cgaatttcac aaaggagact tcctggggtc
     1921 agtgggtact ttctcgtata taggcagata ctatgcctct gtgcccgaaa tggtggttca
     1981 gtcacaaatc tggactgcac gcgcttacac cgaaatgggc tggatttacg aagctgaaga
     2041 tttactccag aaagtcaatc aggatcagat taccggaaaa aatacaggtc tttatgccgc
     2101 ggtaaacgcc aatctgatga taaaaaaagg tcagtacaaa gaagctatcc cgtttctaaa
     2161 actggctatt gaacatgaaa aaaataaaaa aaaccgtacc cgtttcaatt ttgtaatggc
     2221 acaactgcaa accattgcag gtgacaaaaa agcagcatct gattattaca ccgaagtcat
     2281 caaatcgaat cccggctatg aaatgacttt caatgcccgc atcaaccgtt cacaacttct
     2341 ggccgtaaac ctccgcagcg ttgaaaaaga attgaaaaaa atggcacgta atcgcaataa
     2401 ccgtgattat cttgaccaga tttatgctgc cacgggaaat gtttatcttc agtcaaaaga
     2461 tactgcgaat gccatcgaaa attacaatct ggcgcttgaa aaaagcaaac gtaacggaat
     2521 tgataaggct gtagctgcac tcaaactcgg agacctttac tacaaccgta aagattacgt
     2581 aaaatctcaa ccctgttacg acgaagcttc gaaaatcttt accaatgatt ataccgatta
     2641 tgcgcgcatc agcaaacggg ctgaagtatt gggcgaactg gtcaaagagc atgaaataat
     2701 cgtactgcag gatagtctgc aggcactggc attgatgagc gaagacaaac gcaaggaagc
     2761 tatcaaccgc gtcatcgaaa aagttaaaaa agaggaaaaa gaagctgctg aaaaagaaaa
     2821 aaatccacag ggacagggtg gaatgtatga tactgaagat aactttacaa tgcctatcgg
     2881 cactccaatc ggaggaggca acaacagaaa ctggtatttt tacaatccgg tgactatcag
     2941 ctcagggaaa gccgatttca ggaaaaaatg gggaaaccgc aaactggaag acaactggcg
     3001 ccgaaacaat aaatccgcag cactttttgc tgacaatgca gccggtgcta ctgcaactgc
     3061 agttagtacc ctcaccgcgg gaaacgattc tacaaaattg ccaaaaataa aggccacttc
     3121 cgacaaaaca agcgtcgatt attacttgaa tcaaatacca tttaccaccg aacaacggaa
     3181 aaaatcggat gctgaagtgg ctgatgccat gttcaataaa ggcttgattt tcaaagataa
     3241 gctggaagat tttcagatgg cctataatac tttcgatgaa ttcgaacgcc ggtttggcaa
     3301 ggacgaccgg atactcgaaa cactttttca gcgctatctg atggcttcca gagagaagaa
     3361 tgaacttctg gctgagcaat accgcaatcg gatactgagc aattatcccg aaagcaaata
     3421 cgcgcaactt atcaaagatc cgaactatat acttaagcag ggtatgattc tcgccgaaca
     3481 agattctctt tactcacaga cttataccgc ctatacacgc agcgatttcc ctgccgtatt
     3541 cagaaataca gagaatttcc ggaaaaaata tccggtatcg tctctgctgc caaaatttga
     3601 attcctgaat acattgagca ttggaaaaac agccacttcc gttgattttg aaaaatcgtt
     3661 gagtaaactg gttgaaaatt accccgaaag cgatgtcagc gccatgtcga aagatattct
     3721 ggcactgatg aaacagggta atctggctca gaaaggaacc acacacggcg aattactcag
     3781 taagcgtgaa gctgccgata aaactacgga aaagcaggat gatatgagtt ttactgctga
     3841 aaaacagggt aaacaccgct tactactgct gactgatgcc acaacggaag gagtaaacaa
     3901 actcctgtac aacctggcga tatttaattt ctcccggttt atgattaaag acttcgattt
     3961 tattgttggt caggccgatg ctacccatca gagcatatcg gttaccaacc tcgaatcata
     4021 cgatgaagtt atctggtatc agaaaacact taaaaccgat ctggctttat ccgctctgat
     4081 ggactcgctt aaagtacaac aggttgccat ttctgaggat aatttcggaa aaattaaatc
     4141 cattttcact ctggatgaat atcttgcatt cgaaaaggct aatctgataa aggacaaacc
     4201 ggccaatatc gcatctaaca caacaactgc aacggtcaaa aaagaggaac tgaaaaagac
     4261 actgccagaa attaataaaa ccgtaccagt taaagaaact gtagtcgcca gtgtgacaaa
     4321 accaacaacc gaacagaaga atatttcacc cgctgaaaca aaaacaacac ctatagtccc
     4381 tgaacaaaaa gcagcagcgc agcagacaaa acaggctgaa acaacaaaaa ctgaaacagc
     4441 aaaaaccgaa tcaaccggca aaactccggt aactgacagc aaacagcaga caacgccgga
     4501 aaacgtaaaa gccacaacag aaaagatgcc agagaaaccg gctgtagttg aagaaaaagt
     4561 ggaatggtac aaaaatttgt ttgctatccg actgaatgca gaacattttg tggcattcta
     4621 tcttccgccc ggaaaatttg attttgtcaa aattcagaaa gcccttgatg cctacaatgc
     4681 tgccaattac aacatgatga acctaaaagt cagtttggaa gactccggaa aagataaatt
     4741 tatcattgtg ggcgctttcc cggacgccaa cgtggcaaaa tcatatttcc tgcgtatgct
     4801 gaaagatccg gctatcatcg aggcattcaa aggagtgaac agacgcaacc tggttggaac
     4861 caaagaaaac ctgaatataa tggtgaaaaa agatgctctg aatacatact ttgaatttat
     4921 gagagaatat tatttgaaat agaaacgagc cgacgttcag gccaagctca atcatttcag
     4981 gcatctcaca ttcattacac tacattttta caaaacatat caacctacag gggtaacagg
     5041 aggaataatt atgaaaaaag accgcatact ttgggcagac gatgaaatgg atttactgaa
     5101 accttacctg ctctttctgg aagaaaaggg atacgaagta gtcgctgtca ccaacggtaa
     5161 ggacgccata gaattatgcc agaacgaaag ttttgatata atttttctgg atgaaaatat
     5221 gcccggactt agtggtctgc aaacgctggc tcaaatcaag accatcgatc ccaatgtccc
     5281 tatggtcatg attaccaaaa gtgaagaaga ggatatcatg aatatggcca ttggcaataa
     5341 aatagccgat tatctgacaa aaccggttaa tccgagccag attctgctga cgctaaagaa
     5401 aaatattcat aaaaaagaaa tcgtttccga acatgccaca agcagctatc agcaggaatt
     5461 ccgcaacata ggaatgcaga ttaacgattc cttcacctac caggattggg tggatgttta
     5521 taaaaaactc gtttactggg agttggaact ggccgaaacc gaaaacggta tggatgaaat
     5581 gctgaaaatg cagaaaaacg aagcaaacag cacattcaac aagttcgtga agaaaaatta
     5641 cctggactgg atgaccaatc ccgaccagcg accgcttatg agtaccgatt tgttcaaaac
     5701 aaaggtattt ccacttattg acaaaggcga aaaagtgttt tttattgtgg tggataattt
     5761 tcggtatgac cagtggatta tcatccgtga gctgctcagc gaattttata ctttcaacga
     5821 agaagaaacc tactgcagta tccttcctac agctactcag tatgcccgaa atgccatttt
     5881 tgccggactg atgccactgc aaattcagca aatgtatcct catctgtgga tagaagaaga
     5941 ggacgaagag ggtaaaaata tcaatgaaga agcactgctg aaaactcaac tggaccgttt
     6001 ccgcaagaaa tacagtttct cgtaccacaa ggtcttcgag tcgggttttt gtgaaaaggt
     6061 aatcaatcaa cttccttccc ttgaaaataa tcaactgaac gtagtggtgc tgaattttat
     6121 cgacatgctc tcacatgccc gtaccgaaag taaaatgatg cgggaactgg caaacgacga
     6181 agaggcttat cgttcgctga ccttgtcgtg gttcaggcat tccgctacct atgatttgtt
     6241 taaagctatt gctcagcgtg ggtacaaagt agtcttcact accgaccacg gcaccataca
     6301 ggtaaataat gccattaagg tggtaggcga taaaaatgtg aataccaatc ttcgttataa
     6361 agttggaaaa aatctgggat acaacaaaaa agaggttttt gaaatcactc agcctgccaa
     6421 agcgggtctt cccagcccga atgttagttc tgcctatatt tttgcaggta atgacgattt
     6481 ctttgcttat ccaaataact tcaattacta cgtaacctac tacaaaaata catttcagca
     6541 cggcggaatt tcactggaag agatgatcat tccgattgtg acaatggagc caaaataaaa
     6601 agatcagccc actaa
//
LOCUS       DAOPYB010000170         6597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_14240_length_6597_cov_8.680679, whole genome shotgun sequence.
ACCESSION   DAOPYB010000170 DAOPYB010000000
VERSION     DAOPYB010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14240_length_6597_cov_8.680679"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKH58_10900"
     CDS             <1..544
                     /locus_tag="PKH58_10900"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89583.1"
                     /translation="QTMVGLPGENFEHDEYTAACVIGLKPDCVRIYPVLVLKDTELER
                     MSEEGKYVPMSLNAAVERVAAVLNMYINNNINVIKVGLHPSDEYGPGGKLVAGPFHPS
                     FREMAMTAIWKHEFSQKLLSGQKPDKSRDLTISVPDSQYNYAIGYKSENKCYLKQYFN
                     RVRFIRSDVLHKLDFNVDYR"
     gene            528..1220
                     /locus_tag="PKH58_10905"
     CDS             528..1220
                     /locus_tag="PKH58_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89584.1"
                     /translation="MWIIVDKRIPAGAKEKLSRYGSLMEFESSGIVYDAISGHPDIFM
                     CHTDTGLILAPDTPKVYFDFFTKQNIPFKTGKKILGKKYPATSHYNAVVSGKYLIHNL
                     KYTDTAILDVCKQKIHLDTKQAYTRCNLLALDEHNFITSDRNIEKILLKQSLNVLFVC
                     PGEILLPGFANGFFGGCCGVCQKKIYVIGSLTYLNSKNDVIGFIERCGFEVIELYDGK
                     LFDAGGIFIINS"
     gene            1279..3246
                     /locus_tag="PKH58_10910"
     CDS             1279..3246
                     /locus_tag="PKH58_10910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX89585.1"
                     /translation="MKLSVIIVNYNVKYFLEQCLHSVLKAGQGIAMEVFVVDNNSVDG
                     SVSMVREKFPEVKVIENSDNKGFSKANNQAIRQSTGEYVLLLNPDTVVEEDTFSGIIG
                     FMDSHADAGGLGVKMLDGKGRFLPESKRGLPTPAVAFYKVFGLAKLFPKSKIFGKYHL
                     GYLDKDKVHEVEILSGAFMLLRRAALDKAGLLDENFFMYGEDIDISYRITQAGYKNYY
                     FPETRIIHYKGESTKKSSVNYVIVFYNAMIIFARKHFSQKNASSLSFLIKAAIFFRAI
                     LAIVSRFIKLVFVPLLDAAIIFAGIYFIKNYWESNVIFRDGGSYPIEFIAIAVPVYIL
                     VWLFSVYFSGGYDKPVRPIKIIQGVVIGTVLILTVYALLGEQMRFSRALIIFGAGWAA
                     LSMVGTRVLLHLLNVKQYKFGSGKNRRFIIIGDDEEAERVAVLLQKTLINYGFIGLVN
                     TNEKYDAAKGFIGNISQLADIIYIYKIDEAVFCSKSMPPQLIIDKMTELQHTNIDYKI
                     APPESMSIIGSNSINTSGELYVLNVNAIGKISNKRSKRLLDLIISILFMAFYPLLVFF
                     VKHPLGLLKNIFKVLTGIKSWVGFSKSGAGEIHKLPAIRKGVLNPTDAIKKQHISDDI
                     ASSLNILYAKDYKIINDLNIILKGFSKLGRK"
     gene            3436..6594
                     /locus_tag="PKH58_10915"
     CDS             3436..6594
                     /locus_tag="PKH58_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX89586.1"
                     /translation="MTCSLNSRAQISVINGINCNGDTSGILIGFPAGWGTLPYSYLWS
                     TGDTSQAITNLSAGTYTVTITDGWSNDSVFSVTLTDPPLLTTMIYSTTPVSCFNGYDG
                     MATVVPTGGTSPYSYNWSNGINNASATGLSSGIYSVTVTDFNGCTYINSVTITQPAQV
                     TSVISSTIDVTCHGGSNGSANISAAGGIPAYTYSWSNGSSGNLIAGVTAGLYNVTITD
                     ANGCTTGNSVFINEPTPINLPLTSQTDVSCFGGNNGSVTIIANGGFAPYTYLWSNGDT
                     TPTINNLVAGTYSLVVNDSTGCAANTSVTVSQPAPLTINIDQITPSDCDGHDNGGVQI
                     SIPGGVAPYNYYWREINFDSIYTTQDISNVRGGDYEIKVTDILGCTGIDTIIIPNNSF
                     VPVTINYQEYICNGALGSVSILADSADSLQYYTYNWTSTYNTGSFITNDSVYATSTSF
                     QAGNYMIYVTELSTGCVNYFDFTINQSATPMVVTSTVTHNTCYGNHGGSIRLYVNGGD
                     PLPGYHCSWTGPYGFTSTSFVIGGLLSGDYNYTVTDDSSCSQSGTIRIQPLLPLQGYV
                     TKSDVSCYGGTNGIASAYYSGGQGTIHYLWSNASITPQIDNLTAGMYTLTITDSLGCH
                     RHDTAVITEPPQLNISVDSVNHVSCYGYSDGNIWTTATGGSGHLAYTWLLNGSLFPEV
                     TDDIENISAGNYLLTVTDSLLCTAFQDIIINEPAQTFFTDSIHVISCNNGADGYWQIT
                     PAGLYTPYIAIFNTGDTISTDTVPSPFTDGLNAGTYSCQITAANGCLFDFNMSLQQPL
                     PITVGLSNIIPVICYSDSTGSILLDDVHGGKAPYSYAWSNGLTSNPANGLVAGIYNVT
                     ITDSNNCTIEETYTVDQPYEPVKFFPTVYNTSCQQAEDGAVAVNLENIFWSPFNNVFY
                     LYDSTGILIDSVSPGVPIGNLPPGHYTGTIINENGCRATNLIYVDKGPDDCIKIPNLV
                     TSNGDGFNDVFRVEGGCDYDDFKIVIFTDLGKKVFESENCSFAWDPKDVKASANTVFY
                     YYIQVTEKGRVYDFRNSININY"
BASE COUNT         1918 a         1329 c         1389 g         1961 t
ORIGIN      
        1 tcaaacgatg gtgggcctgc ccggagaaaa ttttgaacat gatgaataca ccgctgcatg
       61 tgttatcggc ctgaaacctg attgtgttcg tatttatcct gttttggttt taaaagatac
      121 ggagcttgaa agaatgtcgg aagaaggaaa atatgtgccg atgtcattaa acgctgctgt
      181 tgagagggtt gcagcagtgc ttaacatgta tatcaataat aatatcaatg tcattaaggt
      241 cggcctgcat cccagtgatg agtatgggcc cggggggaag cttgttgcag ggccttttca
      301 tccttccttc cgcgaaatgg ccatgacggc aatatggaaa catgaatttt cgcaaaaatt
      361 attgtccggg caaaaaccgg ataagagccg ggacctgacg atcagtgttc ctgattctca
      421 atataattat gctattggtt ataaatctga aaacaaatgt tatctaaagc aatactttaa
      481 cagggtacgt ttcatccgtt cggatgtact acataagctg gatttcaatg tggattatcg
      541 ttgacaagcg gataccggcc ggggcaaagg aaaaattaag ccgctatgga agcctgatgg
      601 agttcgaaag ctccggcatt gtttatgatg ctatcagcgg gcatccggat atttttatgt
      661 gccacacaga tactggtttg atccttgctc cggatacgcc aaaagtttat tttgattttt
      721 ttactaaaca aaacatccca tttaaaaccg ggaaaaagat cctgggaaaa aaatatccag
      781 ccacttctca ttacaatgcc gtagttagcg gtaaatacct gatccataac ttaaaatata
      841 cggacaccgc cattcttgac gtatgcaaac aaaaaattca cctggacact aagcaggctt
      901 atacccgctg caatctttta gctcttgatg aacataattt tattacttca gaccgaaaca
      961 tagagaaaat cctcttaaaa caatcgctga atgtgctttt cgtctgcccc ggtgagatac
     1021 tgctccctgg ttttgctaat ggcttttttg gcggctgttg cggagtttgt caaaaaaaaa
     1081 tatatgttat cgggagcctt acatatttaa attcaaaaaa tgacgttatt gggtttattg
     1141 aacgttgcgg gtttgaagtt attgagttat acgacggaaa actgtttgat gcagggggca
     1201 tatttattat aaactcataa aatcttttgt caatagttca gtaaaagcta cttttgaact
     1261 caaattgcag gccgtatcat gaaactgtca gtcatcatag ttaattacaa tgtaaagtat
     1321 tttctggaac aatgcctcca ttctgtatta aaagcagggc agggcattgc catggaagtt
     1381 tttgttgttg ataacaattc ggttgacgga tcggtaagca tggtcaggga aaaattcccg
     1441 gaggtcaaag ttattgagaa cagcgacaac aaaggttttt caaaagcaaa caaccaggcc
     1501 atccgtcaaa gtaccggtga atatgtgttg ttattaaatc cggatacggt tgttgaagaa
     1561 gatacttttt cggggatcat cggctttatg gattcccatg cagatgccgg cgggctggga
     1621 gtgaagatgc tcgatggcaa aggacggttc ctgcccgaat cgaaaagagg cctgcccaca
     1681 ccggcggttg ctttctataa agttttcgga ctggcaaaac tttttccgaa atcgaaaatt
     1741 ttcgggaaat atcaccttgg ctatctcgat aaagacaagg tgcatgaagt tgaaatactt
     1801 tccggcgcat ttatgttgct tcgcagggct gcgcttgaca aggcagggct gctcgatgaa
     1861 aattttttca tgtatggcga agacatagat atttcgtacc gtattacaca agccggctat
     1921 aagaattatt actttcccga aacccgcatt atacactaca aaggtgaaag caccaaaaaa
     1981 agcagtgtta actatgttat tgtgttttac aacgcgatga tcatttttgc acgcaaacat
     2041 ttttctcaaa aaaatgcaag ttctttatct tttctaatca aagcagccat atttttcagg
     2101 gccatcctgg ccatcgtcag ccgttttata aaactggtat ttgtaccact gcttgatgct
     2161 gccataattt ttgcaggaat atatttcatt aaaaactact gggaatcaaa tgtcattttt
     2221 cgtgacggtg gctcttatcc cattgaattc atcgctattg ctgtccctgt gtacatcctg
     2281 gtctggcttt tttctgttta tttcagcggg gggtacgata aacccgtgcg tccaattaag
     2341 atcatccagg gtgtcgtcat tggcaccgtg ctgatcctta ccgtttatgc gttattaggt
     2401 gaacagatgc gtttttccag ggccttgatt attttcgggg caggctgggc tgcattatcc
     2461 atggtcggta cacgtgtttt gttgcacctg ctgaatgtta aacagtataa atttggctca
     2521 ggtaaaaaca ggcgttttat cattattggc gatgatgagg aagcagaacg tgtagccgtt
     2581 ttgttgcaaa aaacactgat taattatggt tttataggac tggtaaatac taacgaaaaa
     2641 tatgatgccg ctaaagggtt cattggaaac atttctcagc ttgccgacat tatttacatc
     2701 tacaagatag acgaagccgt tttctgctca aaatcaatgc cgccgcagct aatcatcgac
     2761 aagatgactg aattgcaaca cacaaatatt gactacaaaa ttgccccgcc cgaaagcatg
     2821 tcgatcatcg gaagtaattc aatcaatacc agtggcgagc tatatgtatt gaatgttaac
     2881 gccattggca agatatccaa caagagaagc aagcggcttc tggatttgat aataagcatc
     2941 ttgtttatgg ccttttaccc gttactggtt ttttttgtta agcacccact tggcttatta
     3001 aaaaatattt tcaaagtcct gaccggtata aaatcatggg tggggttcag caaatcgggc
     3061 gcaggagaga tacataaact gcccgccata agaaaaggag tgctgaaccc gacagatgca
     3121 ataaaaaaac aacacatttc tgatgatatt gccagcagcc tgaatattct ttatgccaag
     3181 gattataaaa tcatcaacga tttaaatatc atcctgaagg gattttcaaa gctcggacgc
     3241 aaatagaatt tttgcgtttc acaacttaag aacatttgtg gttaataagt ttatttgtct
     3301 tttttgacat cttcatcaat tttttctatt tttatgtcgg acatttagat attattacac
     3361 gtattaaata tataattata gaagcctatg aaaattaaaa attaccttaa gtaccttctt
     3421 gttataacag ttgttatgac atgttcgctt aacagcagag cacaaatttc agtaataaac
     3481 ggcataaatt gtaatgggga tacttcgggt atcctgatag gttttccagc aggctgggga
     3541 acacttccat attcatattt gtggagtacc ggtgacactt cgcaagcaat caccaacctg
     3601 agtgcaggga cttacaccgt gaccatcaca gatggttggt ctaatgattc cgttttttct
     3661 gtaacattaa cagacccgcc tttgttgacc accatgatct attctacaac gcccgtatcg
     3721 tgtttcaatg gttatgacgg tatggcaaca gtggttccaa caggaggtac cagtccgtat
     3781 tcttacaact ggtcaaatgg aataaacaat gcttcggcta ccggattgag cagtggtatt
     3841 tattccgtta ccgtcacaga cttcaatggt tgtacgtaca tcaattcggt tacaattacc
     3901 cagccagcgc aagtgacatc cgtaatttca tcaactattg acgtaacctg ccatggaggt
     3961 tcgaacggtt cggcaaacat tagtgcagcg ggggggatac ctgcttatac ctatagctgg
     4021 tcgaatggct cgtcaggaaa tttaattgcc ggagttactg ctggtcttta caatgtaacc
     4081 attacggatg ctaatggttg tacaacaggg aattcagttt ttattaatga accgaccccg
     4141 atcaacctgc cgcttaccag ccaaaccgac gttagctgtt ttggcggaaa caacggttcg
     4201 gtaaccatca ttgctaatgg aggatttgca ccctacacct atttatggtc caatggtgac
     4261 acgactccta caataaataa tctggttgca ggtacgtatt cgctggttgt taatgattct
     4321 accggttgtg cagccaatac ttcagtcacg gtgtcgcagc ccgcacccct tactatcaat
     4381 attgatcaga taactccaag tgattgcgac ggacatgata acgggggagt acaaatttca
     4441 attccgggag gtgtggctcc gtataattat tattggcggg aaataaattt tgattcaatt
     4501 tacaccaccc aggatatctc aaatgtacgg ggtggtgatt atgaaataaa ggtaactgat
     4561 attttgggat gcactggcat agacacaata ataattccca ataattcttt tgttcctgtt
     4621 acgatcaact accaggaata catatgcaac ggggcgcttg gttcagtaag cattctggca
     4681 gacagcgccg atagtcttca atattacact tataactgga catctacata caatactggt
     4741 tcatttataa ctaacgactc tgtatatgct acttcaacca gttttcaggc aggaaattat
     4801 atgatttacg ttaccgaact cagcactgga tgtgttaact attttgattt tacgattaac
     4861 cagtctgcaa cacctatggt tgttacatca acagttacgc ataatacatg ctatggcaat
     4921 catggcggat caataaggct atacgtaaac ggtggcgatc ctttgcccgg ctatcattgc
     4981 tcatggacag ggccatatgg attcacctcg acatcttttg taataggcgg gcttttatcc
     5041 ggcgattata attacactgt taccgatgat agttcctgtt cacagtcggg gactatacgt
     5101 attcagccat tgttacccct gcagggttat gtaaccaaaa gtgatgtgtc atgttatgga
     5161 ggtactaatg gcatagcgtc ggcatattat tcaggcggac aggggactat ccattatttg
     5221 tggtcaaatg caagcattac gccacaaata gataacctga ctgcaggaat gtataccctg
     5281 acgatcaccg acagtcttgg ttgccacagg cacgacactg ctgtgataac ggagccgccg
     5341 caattgaata tatcggtaga ttctgtgaat catgtgagct gctatggata ttccgacgga
     5401 aatatctgga cgacggcaac cggaggatca ggacatctgg catatacatg gcttttaaac
     5461 ggcagcctgt ttcctgaagt aactgatgat attgaaaata tttctgccgg taattactta
     5521 ttaactgtca cagattcact tttatgtact gcttttcagg atataattat caatgaacct
     5581 gctcaaacat tttttaccga cagcattcac gtgataagtt gcaacaacgg ggcagacggc
     5641 tactggcaaa taacacctgc cggattatat acaccttaca tcgccatttt taataccggc
     5701 gataccatca gcacagatac agtgccttct ccttttactg atggacttaa tgccggcact
     5761 tattcctgcc agataactgc tgccaacgga tgcctttttg attttaatat gagccttcaa
     5821 cagccattac caataaccgt tggtttaagc aatattatcc cggtaatctg ttatagcgac
     5881 agtacaggtt cgatattgct cgatgatgtt catggcggta aagctccata ttcttatgcc
     5941 tggagcaacg gattgacaag caatcccgca aatggtcttg ttgcaggtat ttataatgtg
     6001 acaattaccg actcgaataa ctgtactatc gaggaaacct atactgttga ccagccatac
     6061 gaacccgtca agtttttccc aacggtttac aatacatcct gccagcaagc cgaagacggc
     6121 gctgttgctg tgaatcttga aaatatattc tggtctccat tcaataatgt tttttatctg
     6181 tacgattcaa ccggtatttt aattgatagt gtcagccccg gtgttccaat cggaaatctt
     6241 cccccgggac attatacagg tacaattatc aacgaaaacg gttgtagggc aaccaattta
     6301 atatatgttg ataaaggccc cgatgactgc attaaaatcc cgaaccttgt tacgtctaac
     6361 ggagatggat ttaatgacgt attcagagtt gagggtgggt gcgactacga cgattttaag
     6421 atagtaattt ttacagacct tggtaaaaaa gtttttgaat ctgagaactg tagctttgcc
     6481 tgggatccaa aagatgtcaa ggcatctgca aataccgttt tctattatta tattcaggta
     6541 actgaaaaag gccgggttta tgatttcaga aattcaatca atattaacta ttaaaca
//
LOCUS       DAOPYB010000171         6591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_14261_length_6591_cov_9.069308, whole genome shotgun sequence.
ACCESSION   DAOPYB010000171 DAOPYB010000000
VERSION     DAOPYB010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14261_length_6591_cov_9.069308"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKH58_10920"
     CDS             complement(<1..406)
                     /locus_tag="PKH58_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX89587.1"
                     /translation="MKPEVITNKFNAEIKPRQAGQRHVLKLACEPLPNVRIALVGLGT
                     RGSLAVNRFLNIEGAQIAAICDIKKENLQQVQRQLKKHHQNPASEYIVADDWRKACEH
                     PDIDLIYICTDWLSHVPIAVYAMQHGKHVAIEV"
     gene            complement(540..2600)
                     /locus_tag="PKH58_10925"
     CDS             complement(540..2600)
                     /locus_tag="PKH58_10925"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNX89588.1"
                     /translation="MKFKITQETIKLITKLGLFIALIAIIVQLFPKDKKFKYQYEVGK
                     PWTYELITASYDFPIYKTEKQLEADKEELLQNYAPYFQLDENITKRQINQWRTDWKLK
                     NNTDSRYDAYVERKLNDIYSSGVVSPTTFDRLKSLGRKNIVVVKSNRLTSKVPVDELY
                     TPKEAYEEIVLDKPYYFSDSELSKYNLNLYLVENLRYDSITSEAVKQEMFKNLSLTSG
                     MVQAGERIIDRGEIITPGTFSILNSMKIASEKRKTAFHESYLVLIGEFIMIFMFILLL
                     TFYLYLFRPYIYGSAKNLFFIALMMLLIVALSSLVLRFTSLSIYIVPFALLPVIIRVF
                     FDSRTGLFAHIITVLIISFMVDNPFLFIILQITAGMAAVSGLKDMTQRSQLTQTAFYI
                     FLSYTVMYLASEFIAEGDLTRIHFLPIINFGVSSLLLLFAYVLIYILEKIFGLISAIT
                     LVELTNINSDLMMKFAELAPGTFQHSLQVSNLATEAAKKINANSLLVRTGALYHDIGK
                     MKNPQYFIENQEGGINPLLDMSYEEAAKAVVSHIADGVAIAKKYRLPEQIVGFITTHQ
                     GKSKTKYFYNSYINANPGKSPNEEDFMYPGPIPYSKETAILMMADAVEARSRTLDQYT
                     DQSISEMVENMIDTQIADGQLKDAPISFRDVETVKKVFTEKIKNIYHNRITYPELKNQ
                     PEKE"
     gene            2895..3668
                     /locus_tag="PKH58_10930"
     CDS             2895..3668
                     /locus_tag="PKH58_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNX89589.1"
                     /translation="MFTIITLPLIPLRAEADERSEMVSQLLFGQQVEILETTEGWYQV
                     KNLADNYTGWIGKKTVSSRYFTSDPVDTSAFRAAKTPLVVCFRTSSVEKITLPGGSLL
                     PPVTREQFELQNEIYQLAQFEPVFTKNNNGQLVVELANQYVNAPYLWGGKSIFGIDCS
                     GLVQVVFSMIGISLPRDASQQVEIGTVVDFLSEVEAGDVAFFENEEGNIVHTGILINS
                     HQIIHSSGNVKIEIIDSQGIISSQTGEYTHKLRVIKRIV"
     gene            3971..4501
                     /locus_tag="PKH58_10935"
     CDS             3971..4501
                     /locus_tag="PKH58_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX89590.1"
                     /translation="MEAQKFNSIFSALTGKLYRFAKSMLRNSVEAEDALQDVQLKLWE
                     KRSELDVVENMDGFAMRVMRNRCLDIIRQRRETSELHPELISDHKNPHEQAEVKNMTE
                     RVKLLIDRLPELQRSIIRMRDVEEMETSEIAYIMDMTENAVTVNLSRARSKIKTQILN
                     ELQAENNTIWKEQMNY"
     gene            4477..4983
                     /locus_tag="PKH58_10940"
     CDS             4477..4983
                     /locus_tag="PKH58_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89591.1"
                     /translation="MERADELLNKYFGGTSTLSEEKELKDYFNSKRVSENHVIYKPLF
                     QQFTAEKEEIYPEVAPILRIEKPKNNRNKILIWTISGVAACLLAITLVKPLMQSEDYL
                     IMKGKRIDNPELAQQIAIKKMEKSLSMVNRRLEPVEKLGEVEMKLEKIEKISQIRQNV
                     QNKLSKIK"
     gene            5087..5566
                     /locus_tag="PKH58_10945"
     CDS             5087..5566
                     /locus_tag="PKH58_10945"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNX89592.1"
                     /translation="MKKLIMAFVAIIIFTSAAQAQSLDKFFKKYSDDERFQYVSVGSG
                     AMNIASAFGGVDRETKKMISKMKGIKILTLEQGSNTALANAFINELNEIVSKGDFETV
                     VEAREKNERTYIYKRVDSKNNADMLIMSKEPNETNVIWLSGKMSPEELSKMMNEGNQ"
     gene            5928..>6591
                     /locus_tag="PKH58_10950"
     CDS             5928..>6591
                     /locus_tag="PKH58_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89593.1"
                     /translation="MKTYNTKSLFIRYYLPIFLVFSISVLAFQYEREKRYKADVLDMR
                     LEDTNAAIFYFLEHSEGSLNHLDSLIKNSTFKDERITIIDLSGKVVYDDVVRDVDRMD
                     NHLSRNEVKQARKKGSGSDIRTSKTNNKPYYYHATRFDNCYVRSSLPYDMTILSLLSP
                     DNLFLYFWLVLTFIIIAVLFYFSNRFTIKMRHEQAEQNSLVRRKLTQQVAHELKTPLS
                     SII"
BASE COUNT         2037 a         1298 c         1357 g         1899 t
ORIGIN      
        1 ggacttcaat tgccacatgc tttccgtgct gcatggcata aacagctatc ggaacgtgcg
       61 aaagccagtc ggtacaaata tagatgaggt caatatcggg atgttcacat gccttgcgcc
      121 agtcatcagc tacaatgtat tcggaggccg gattttggtg gtgtttttta agctgtcgtt
      181 gtacctgttg gagattttcc tttttaatgt cacagattgc agcaatttgt gctccttcaa
      241 tattcagaaa acggttcaca gccaggcttc ctcttgtgcc taatcctacg agtgcaattc
      301 tgacattggg caaaggctcg caggcaagct tcaacacatg cctctgacca gcctgacgag
      361 gtttaatttc cgcattaaat ttattggtta taacttccgg tttcatgatc ttgatattgg
      421 cacaaaaata attcaatttt tctttttact aaatttattt gactgcaatg taaaaaaaca
      481 acctgccgat gtttgcatca gcaggctgta agttatatta tttggttggt ttgaatatcc
      541 tattctttct ccggttgatt tttcagttcc ggataagtaa ttctgttgtg atatatattt
      601 ttaatctttt ccgtaaatac cttttttact gtttctacgt ctctgaagct tatcggggca
      661 tctttaagct gcccatcggc aatctgcgta tcaatcatat tttcaaccat ttcgctgatg
      721 ctttgatcgg tatattgatc aagtgtcctg gagcgtgctt caaccgcatc ggccatcatc
      781 aggatggcag tttctttgct gtaaggtatc ggacccggat acataaagtc ctcttcgttg
      841 ggtgatttac ccggatttgc atttatgtaa gaattataaa aatactttgt tttagatttt
      901 ccctgatgtg ttgtgataaa tccgacaatt tgttctggca gtctgtattt ttttgcaatt
      961 gccaccccat ctgcaatatg acttaccaca gctttggcag cttcttcata actcatatcc
     1021 agcagaggat ttattccacc ttcctgattt tcgataaaat actgcggatt tttcattttt
     1081 ccgatatcgt gatataacgc tccggtacga accagcaaac tgttagcgtt aatttttttt
     1141 gcagcttcgg tagccagatt cgatacctgc aacgaatgct gaaaagttcc gggtgccagt
     1201 tcggcaaatt tcatcatcag gtcactgttg atgttcgtta attctacaag tgttatggcc
     1261 gaaattaatc cgaatatttt ttcaagaata taaattagca cataggcaaa cagcaggagt
     1321 aacgagctaa ccccgaaatt gattattggt aagaaatgaa tacgtgtcag gtcgccttcg
     1381 gcaataaact cggaagccag atacatgact gtgtagctca gaaaaatgta gaatgccgtt
     1441 tgggttagtt gcgagcgctg agtcatatct ttcaaccccg aaacagctgc cataccggcc
     1501 gttatctgta gaataatgaa cagaaagggg ttgtcgacca taaatgagat aatcagcacg
     1561 gtaataatat gtgcaaaaag tccggttcgg gagtcgaaaa acacacggat gataaccgga
     1621 agaagcgcaa acgggactat ataaatactc agactggtga atctcaaaac cagggatgat
     1681 aacgctacaa tgaggagcat catcagagca ataaaaaaaa gatttttggc actcccgtaa
     1741 atgtatggtc tgaacaaata aagataaaac gtaagtaaga ggataaacat gaaaatcatg
     1801 ataaattccc caatcagcac cagataagat tcgtgaaaag cagtttttcg tttttcggat
     1861 gctattttca tcgaattcag gatagaaaaa gttccgggtg ttattatttc tccacggtca
     1921 atgatacgtt ctccggcttg caccataccc gatgtgagtg acagattttt gaacatttcc
     1981 tgtttgacag cttcagaagt gatgctgtcg taacgcagat tttcaaccag ataaagattc
     2041 aggttgtatt tactcagttc actgtcgctg aagtagtatg gtttatccag tacaatttcc
     2101 tcgtatgctt ccttgggtgt atagagctca tccacaggaa ctttactggt caatctgttg
     2161 gatttcacaa caacaatgtt tttacggcca agtgatttca atctgtcgaa ggtggtggga
     2221 ctaacgacac cactgctgta tatatcattc agttttcttt ccacataggc atcatatctc
     2281 gaatcggtgt tatttttaag tttccagtca gtgcgccact ggtttatttg tcgcttggtt
     2341 atattttcat ccagttggaa atagggtgca taattctgca agagttcttc cttatcagct
     2401 tcgagttgtt tttcggtttt ataaatcgga aaatcatacg aagcagtaat aagctcatac
     2461 gtccagggtt taccaacctc gtactggtat ttaaatttct tatctttcgg aaacagttgc
     2521 acaataatgg ctatgagggc aataaacaaa ccgagtttgg ttataagctt aatcgtttcc
     2581 tgtgttattt tgaatttcat acgttaattt ctttttttac atgaattgga ttgaaatcat
     2641 tgatttcgaa ttatattaac ataaagtacg catcgcgaaa ttattctgat tttatgatga
     2701 aaaaacttcg ggcagatcac cgggaaaaac ctgttgaaga ttaaacatca aaaataggaa
     2761 taaaagtata aaaaaaacag gaatatagcc taaaatgttt gaactttccc tatttttgta
     2821 aaaaagaagt atgtatcaat cagtctgaaa ttttcggaca ttttttttga aagtcattaa
     2881 cgcaggtaaa taatatgttt acaattatta cacttccatt aatccccctt cgggctgagg
     2941 ccgacgaaag gagcgaaatg gtttcgcaac tgcttttcgg gcaacaggtt gaaattcttg
     3001 aaaccaccga aggatggtat caggtaaaaa atctcgctga taattacacc ggttggattg
     3061 ggaagaaaac ggtatcctcc agatatttca catccgatcc cgtggacact tccgctttca
     3121 gggcagcaaa aaccccgctg gtggtttgtt tcaggacctc gagtgttgaa aagattacgc
     3181 ttccgggcgg aagtctgctg ccaccagtca cccgtgaaca atttgaacta cagaacgaga
     3241 tttatcagct ggcacaattc gaacctgttt ttaccaaaaa caacaacggg caactggttg
     3301 ttgagctggc caatcagtac gtaaacgcgc cctatctctg gggtggaaaa agtatcttcg
     3361 ggattgactg ttcgggactg gtacaggtag tcttttcgat gataggcatt tcacttcccc
     3421 gtgacgcgtc gcagcaggtc gaaattggta ctgtggttga tttcctctca gaagttgaag
     3481 ccggcgatgt ggcatttttt gagaatgagg aaggcaacat cgttcatacg ggtattctta
     3541 tcaactcaca tcagattatt cattcgtcgg gcaacgttaa gattgagatt attgacagtc
     3601 agggtatcat ttcatcacaa accggtgaat atacacataa actgagggta atcaaacgaa
     3661 ttgtataaaa gcaattacca acacagtata ctaacggaac gcaagcgata aaattgactg
     3721 attttattct ctgcgttctt ttcttttaca cgaaaatgcc cccagcctcc tgaaggggga
     3781 gctgaaagta tttatcgaaa acttacagta gaattggatg tgcattaaat gtttttatcg
     3841 gaggaggtgt gatatttttt atcaggaaaa atatgttttt attccatatt ctgaaaacaa
     3901 atttttccga aaataagaaa atttatttcc cggtgtaata ttttttccga cctttcgtca
     3961 ttaatagtgt atggaagctc aaaagttcaa ctcaatattt tcggcactta ccggtaaact
     4021 ctaccggttt gcaaaatcaa tgcttcgaaa tagcgtcgaa gcggaagatg ccttgcagga
     4081 tgtacagttg aaactttggg agaaacgcag cgaactggat gtggtggaga acatggatgg
     4141 ctttgcaatg cgcgtgatgc gaaacaggtg tctggacatc atccggcaaa gacgtgaaac
     4201 cagcgagctg cacccggaac tgatcagcga ccataaaaat ccgcatgaac aagctgaggt
     4261 gaaaaacatg acagaaaggg taaagctcct catcgaccgg ctgccagaac tgcaacgaag
     4321 cattatccgc atgcgcgatg tggaagaaat ggaaacgagc gaaattgcct acatcatgga
     4381 catgaccgaa aatgccgtaa cggtaaacct gtcgagggca cgctcgaaga ttaagacaca
     4441 aattttgaat gaattacaag cagaaaacaa cacgatatgg aaagagcaga tgaattacta
     4501 aacaaatatt tcgggggaac cagcacctta tcggaagaaa aagaattgaa agactatttc
     4561 aacagtaaac gtgtttccga aaaccatgtc atatacaagc cccttttcca gcagtttact
     4621 gccgaaaaag aagaaattta tcccgaagtt gcacctattc tgcggattga aaagccgaaa
     4681 aataaccgca ataaaatact gatatggacc attagcggtg ttgcagcctg cctgctggca
     4741 ataacgttgg taaaaccgct gatgcaatcc gaagattact tgattatgaa agggaaacga
     4801 attgacaatc cggaactggc tcagcagata gccataaaaa agatggaaaa atcgctgtcg
     4861 atggttaacc gcagactgga acccgtggaa aaactcggag aggtagaaat gaaactggaa
     4921 aaaattgaaa aaatcagcca aatcaggcag aatgtacaga ataaattatc taaaataaaa
     4981 taaccgctgc gaaacaggca tttataaagc ttttaaaacc cagggaaaat ggatgtcggt
     5041 ttcgtgaaaa attcagatta aaaacaacta aaaaaagaat aatcgtatga aaaagttaat
     5101 catggcattt gtcgccataa tcattttcac atcagctgca caggcacagt cgctcgacaa
     5161 gtttttcaag aaatactccg acgatgaacg tttccaatac gtatcggtag gcagcggagc
     5221 tatgaacatt gcatcggctt ttggcggagt ggacagggaa acaaaaaaaa tgatttcgaa
     5281 aatgaaggga ataaaaatcc tgacattgga acagggatct aataccgcgc ttgccaatgc
     5341 atttatcaat gaactgaacg aaattgtaag taagggcgat tttgaaaccg tggtggaagc
     5401 ccgcgaaaag aatgaacgaa cttacatcta caaacgcgtt gacagtaaaa ataatgccga
     5461 catgctcatc atgtccaagg aaccgaatga aaccaacgtc atctggctaa gcggcaaaat
     5521 gtcgcccgaa gaactttcga aaatgatgaa cgaaggcaat cagtagggat attaaaagag
     5581 cacaaaacac acaaaagccg acacgatcaa atgtcggctt ttgtgtgtta tttcaagccc
     5641 cctaccccct aaagggagag ttcattgata cgagacaatg attactaacg ttttgcttat
     5701 gattgttgta cttctccaaa cattccaaac ggtttactta ggattaccgc ttatctggtt
     5761 ctttattccg gtttttatta actaatttga gtacctttga tagcgaaaat attttttaat
     5821 cacctcattt tgtgaattta atggttttgt aatatccttt tgtctaaaca tttatatctt
     5881 tgcacagata aaatgttaac atttttagct atcgttaaag aaacaatatg aagacataca
     5941 acacaaaaag cctgtttatc cggtattatc tgcctatttt tctggtattt tcaatttcgg
     6001 ttcttgcctt tcagtacgaa agagaaaaaa gatacaaagc cgatgtgctg gatatgagac
     6061 ttgaagatac caacgcggct attttttatt ttctggaaca ctccgaaggc tcgctaaacc
     6121 atctcgattc cctgatcaaa aattccacat ttaaagatga acggataacg atcatcgacc
     6181 tttcgggaaa agttgtgtat gatgatgtgg ttcgggatgt ggacagaatg gataatcacc
     6241 tatcgcgcaa cgaggtgaag caggcccgca aaaaaggctc gggttcggac attcgcacgt
     6301 ccaaaaccaa caataaaccc tattattatc atgccacccg tttcgacaat tgctatgtgc
     6361 gatcatcgct gccctacgac atgaccatcc tgtcgcttct gtctcccgat aatcttttcc
     6421 tgtatttctg gctggtactt acctttatca ttattgcagt acttttttat ttctcaaacc
     6481 ggtttaccat caagatgcgg catgagcagg ctgaacaaaa ttcactggtt cgccggaaac
     6541 tgacccaaca ggtggcacat gaacttaaaa cgccgttgag cagcatcatc g
//
LOCUS       DAOPYB010000172         6496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_14555_length_6496_cov_11.292812, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000172 DAOPYB010000000
VERSION     DAOPYB010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14555_length_6496_cov_11.292812"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..455
                     /gene="dut"
                     /locus_tag="PKH58_10955"
     CDS             21..455
                     /gene="dut"
                     /locus_tag="PKH58_10955"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNX89594.1"
                     /translation="MKISIVNKSRHNLPEYATPLSAGLDLRANIDEPVELKPLERKLI
                     PTGLFIELPQGYEAQIRPRSGLAIKKGITVLNSPGTIDADYRGEICVIMINLSTEPFV
                     INDGERICQMIIAQHAQAEWIETEVLNETDRGGDGFGHTGRK"
     gene            569..2266
                     /locus_tag="PKH58_10960"
     CDS             569..2266
                     /locus_tag="PKH58_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX89595.1"
                     /translation="MISSTAHALTDDEQARFDYYFYEAIRQKNVGDPGAQMEALRMCL
                     ELDSTNGAVQSETGLAYAGLNMIPEAINAFRKSVDASPANWWYRVQLISMLSSREQYQ
                     DAIIHAEELSKIYPEREAVYTMLSSLYKQTGEYDKAIRALDKLETYVGINEYLSFEKF
                     QLYAALNKAKKAIAEIDKLVAKYPSETRYQVLRGDIFMDQKQPEKAFEIYQRVLADDP
                     ANPFVYISLANYYKEKKQPDKALESIVSALKNPALPSDTKMDILGQYVDKLLSGDQKI
                     GETEELFKLLVEMYPLEENPHAYYAHFLQNQKRFPEALSELQSVIDINPKNETAWLTS
                     LQILSEKEDTLAILDLTKRGIKELPRIPQFYFYKSMAEYQLGDYEASLKTNEEALLNV
                     ESASSAVISSFYGQIGDIYYKLNNKEKAFLNYEKALEQNPANVYVMNNYAYYLSEEKL
                     DLRKAERMSAKTVELEPKNSTYLDTYAWILYQQGNFSLAKFYIERAVDNLEKEHDPGV
                     ILEHYGDILLALKNEKKALEMWQKAWDSGKTDEELKIKIEKANLNIENVKENESNNK"
     gene            2247..3062
                     /locus_tag="PKH58_10965"
     CDS             2247..3062
                     /locus_tag="PKH58_10965"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNX89596.1"
                     /translation="MKVTINKIILLSIVLLAFGACKSKKLLVKSEIPTEMTPAAMVAK
                     LQDKQPAFSSANIKKMTVDVDMKERRMTVNATCKMVTDSAIHLSIQPFFGIELFKLEL
                     TPWNIVLIDKTNKTYYEAQYTQLRSRLGLDFDYKSIQSVISNRLFVSGIKTFLADDYK
                     WKDNDVKSGILTVQGERMNQEIMVDAALDRIIGLVLKTNDGSYTLKTEYSGFKNFDKQ
                     LFPEMINMEAFNGESKASLYFKIEDVEFDKPLVLEAANLRRYQRGNLARFFIK"
     gene            3107..4585
                     /locus_tag="PKH58_10970"
     CDS             3107..4585
                     /locus_tag="PKH58_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNX89597.1"
                     /translation="MKNKFFLLLTFISLCLCYNAALQAQTVDDLQKERKQIQERILNT
                     NKQIKQTEKKEKASLNKLDFLKKNLKERKNLINNYSKEINLLDKKITNLITQKKELED
                     QLEKLKQDYAKLIQKTQANRSSYSKLMFLLSSNNFDQTLRRVRYLQEFTNYRKEQVRK
                     IEQVKQQIALKTDSLDMHKGSKMQAMKSKEMEAAKLKQDESNEKVLLAGLQQEEKKLR
                     DEYRMQQHKRNEIDNRIEQIIAEEIRKAEARRRAEAAEKQAQELRKKLAEERRIKEER
                     DKKAAEDRKMAEAKKAKEKTTSTTAKTETSKIPETKTIADKTSKPEVTTTAARTAETN
                     RESSGTSSVVYEMSREESLLSGGFEQNRGRLPWPVDRGSISGHYGVQPHPVLKHVQID
                     NKGTYFLSPSGTNARAVFEGVVERRFSLPGSGNAVIIQHGNYRTLYGNLTNIYVRTGD
                     HVKAKQAIGQIYTDEENGGKTELIFQIWSGLTRLNPENWITR"
     gene            4622..6487
                     /locus_tag="PKH58_10975"
     CDS             4622..6487
                     /locus_tag="PKH58_10975"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNX89598.1"
                     /translation="MENLIEQEVAEIRYDDANIVTLEGLEHIRRRPGMYIGKLGDGTH
                     SDDGIYVLLKEVIDNSIDEFRMGYGKTIEVSIGEGIVSVRDYGRGIPLNSLVKAVSVM
                     NTGGKFDSQAFKKSVGLNGVGTKAVNALSSRFVIQSFREGQSRRAEFEKGLLQTEELE
                     ELKAENGTLVIFEPDKALFQNYVYNYDIIETMLRNFSYLNTGLTILFNGKKILSKNGL
                     TDLLNENMTAEPLYPIIHLRTEDIEIAFTHSDQYGEEYYSFVNGQHTTQGGTHQSAFR
                     EAVAKTIKEFYNKNHELTDIRNGIIAAVAIGVEEPVFESQTKTKLGSRDIAKDGITVN
                     KFISDFVKKELDNYLHRNSETAEILLQKIQESEKERKAIAGVTKLARERAKKVNLHNK
                     KLRDCRVHYNDTKGSPELLDSSSIFITEGDSASGSITKSRDVNTQAVFSLRGKPLNSF
                     GLTKKIVYENEEFNLLQAALNIEDGIDGLRYNKVIVATDADVDGMHIRLLLITFFLQF
                     FPDLIKKGHVFILQTPLFRVRNKKETVYCYSEEERLAAIARLGANPEITRFKGLGEIS
                     PDEFKYFIGKDIRLDQVTLKKEDAVIDLLAFYMGKNTSERQNFIIENLVVEEEVE"
BASE COUNT         2229 a         1276 c         1405 g         1586 t
ORIGIN      
        1 ttattaaata tcacaagcgc atgaaaatca gtattgtaaa taagtccaga cacaatcttc
       61 ccgaatatgc cacaccgctg tcagcaggtt tggatctgcg ggcaaatatt gatgagcctg
      121 tggaacttaa acctctcgaa aggaagctca tacctacggg attattcatc gaactgccac
      181 agggctatga ggcgcagata cgaccacgca gcggactggc gattaaaaag gggattactg
      241 tattgaattc gcccggaaca atagacgctg attatcgcgg agagatatgt gtgataatga
      301 taaacttatc aaccgaacca tttgtgataa acgatggtga aagaatttgc cagatgataa
      361 ttgcacaaca tgcacaggcc gaatggatcg aaaccgaagt gctgaatgaa accgatcgcg
      421 gtggcgacgg atttggacat acaggcagaa aataagtcag tgaataagaa cttactccca
      481 aggattatta agcatgatta aattaaaaaa actcatattt ttttctgtca tattgttttt
      541 tgcagcttgc agcagtcaaa agtcagctat gatcagtagt actgcacatg cgcttaccga
      601 tgacgaacag gcacgtttcg actactattt ctacgaagcc attcgtcaga aaaatgtggg
      661 tgatccaggg gctcagatgg aagcattacg tatgtgcctg gaattggact ctacgaatgg
      721 tgctgtacaa agtgaaaccg gacttgctta tgccggtttg aatatgattc ccgaagcaat
      781 taatgctttc cgcaaatcgg ttgatgcaag tcctgccaat tggtggtatc gcgtgcagct
      841 tatttcgatg cttagcagca gggaacagta tcaggatgca atcattcatg ctgaggagct
      901 cagcaaaatt taccccgaac gcgaggctgt atatactatg ctctcttctc tctacaaaca
      961 gaccggcgag tatgacaaag ccatacgggc gctcgataag ctggaaacgt atgttggtat
     1021 caatgagtat ctgtcgttcg agaagtttca gttgtatgct gcgctcaaca aagctaaaaa
     1081 agctatcgcc gaaattgaca aactggtagc caaatatcct tccgaaaccc gttatcaggt
     1141 acttcgtggt gatattttca tggatcagaa acagcccgaa aaagcatttg aaatctatca
     1201 gcgcgtgttg gctgacgatc cagcaaatcc gtttgtatat atctcgctgg caaactatta
     1261 caaagaaaag aaacaaccgg acaaggctct agaatccatt gtttcagcat tgaaaaatcc
     1321 tgccctgcct tccgatacca aaatggatat actcgggcag tatgtggata aattactttc
     1381 aggtgatcag aaaataggtg aaacagagga acttttcaaa ctgctggtgg agatgtatcc
     1441 gctcgaagaa aatcctcatg cttactatgc acatttttta caaaatcaga aaagatttcc
     1501 cgaagcactt tccgaactgc agagcgtgat tgacatcaat ccgaaaaacg aaacagcctg
     1561 gcttacttcg ctccagatac tttccgagaa agaagataca cttgcaattc ttgacctgac
     1621 aaaacgagga ataaaagaat tgccaagaat tcctcagttt tatttttaca aatccatggc
     1681 tgagtatcag cttggcgatt atgaagcatc tctcaaaacg aatgaagaag cacttttaaa
     1741 tgtagaatct gcttcatcag ccgttatcag tagtttttat ggccagatag gcgatatcta
     1801 ctataaactg aataacaagg aaaaagcatt tttgaactat gagaaagccc ttgagcaaaa
     1861 tcccgccaac gtgtatgtaa tgaataacta tgcctactac ctgtcggaag aaaaacttga
     1921 tttgcgaaag gcagagcgta tgagcgccaa aacggtggaa cttgaaccta aaaacagcac
     1981 ttatctggat acttatgcct ggattttgta ccagcagggt aatttcagcc ttgccaaatt
     2041 ctatattgag cgggcagtgg acaatctgga aaaagaacat gaccccggag ttattctgga
     2101 gcattacggc gatatacttt tagcgctaaa aaatgaaaaa aaagcacttg aaatgtggca
     2161 aaaagcttgg gattccggta aaaccgacga ggaactcaaa ataaaaattg aaaaagctaa
     2221 tcttaatatt gaaaatgtaa aggaaaatga aagtaacaat aaataaaatc attctgttaa
     2281 gtattgtgct gttggctttc ggagcgtgta agtccaaaaa gctgctggtt aaaagtgaaa
     2341 ttccaactga aatgactccg gcagctatgg tggcaaaact gcaggacaaa caaccggctt
     2401 tcagttcggc taacattaaa aagatgacag tagatgtgga catgaaggaa cgtcggatga
     2461 ctgtaaatgc tacctgcaag atggttacag attcggccat tcacctttcc attcaacctt
     2521 tttttggtat cgaattgttc aaacttgaac ttactccctg gaacatcgtg cttattgata
     2581 aaacgaataa aacctactac gaagcacagt atacgcaatt gagaagtcgc ctggggctgg
     2641 attttgatta taaaagcata cagtcagtga tcagcaacag actcttcgtg tcgggcatta
     2701 aaacttttct tgccgatgat tacaaatgga aagacaacga tgtaaagtcg ggaatactta
     2761 ccgtgcaggg cgaacgaatg aaccaggaaa ttatggttga tgcagcactt gatcgtatta
     2821 taggtctggt acttaagaca aatgatggat cctacacgct gaagacagaa tattcaggat
     2881 ttaaaaattt cgacaaacaa cttttcccag aaatgataaa tatggaagca ttcaacggag
     2941 aatcaaaagc ttcgttatac ttcaaaatag aagatgtgga atttgataaa ccccttgtgc
     3001 tcgaagctgc caacctccga cgttatcagc gtggcaatct cgcccgtttt tttattaaat
     3061 aattcactga ttctctgaaa aagagatagt ataacatatg ataaaaatga agaataagtt
     3121 ttttttactc ctgactttca tttcactctg cctctgttac aatgcagcgt tgcaggctca
     3181 gactgtggac gatttgcaga aagaacgaaa gcagattcag gagcgtattc ttaataccaa
     3241 caaacagata aaacaaaccg aaaagaaaga aaaagcatca ctcaataaac ttgactttct
     3301 gaaaaaaaac ctgaaagaac ggaagaatct catcaataat tacagtaagg aaatcaatct
     3361 gctggataag aaaataacca atctgattac ccagaaaaag gaactggaag atcagctcga
     3421 aaaactgaaa caggattatg ccaagctcat tcagaaaacc caggcaaacc gaagttccta
     3481 ttccaaactc atgtttttac tttcatccaa taattttgac cagacattgc gtcgcgtcag
     3541 atacctgcag gaatttacaa attaccgtaa agaacaggtt aggaaaattg agcaggtcaa
     3601 acagcagata gcactgaaaa ccgatagcct cgacatgcac aaaggctcaa aaatgcaggc
     3661 tatgaaatca aaggaaatgg aagccgcaaa gcttaaacaa gatgaatcaa acgaaaaagt
     3721 gttgttggct ggtctgcagc aggaagaaaa aaaactcagg gatgaatacc ggatgcaaca
     3781 acacaaacga aacgaaatag acaatagaat agaacagata attgcagaag aaatcaggaa
     3841 agcagaagca agacgcagag ccgaagcggc tgaaaaacaa gcacaggaac tccggaaaaa
     3901 attagctgaa gaacgcagaa taaaagaaga acgcgacaaa aaggctgccg aagatcgtaa
     3961 aatggctgaa gcaaagaaag caaaagaaaa aacaacatca actacggcga aaactgaaac
     4021 ttcgaaaata cctgaaacaa aaactatagc tgataaaaca tccaaaccgg aagtaacaac
     4081 aactgcagca agaactgccg aaacaaaccg tgagagttcc ggtacttcat cggtggtgta
     4141 cgaaatgtca agagaagaaa gcctgctttc aggcggattc gaacaaaaca ggggaagact
     4201 tccctggccg gtcgatcgtg gttcgatcag tggacattat ggtgtacagc cacatccggt
     4261 tcttaaacac gtacaaattg acaacaaggg aacatatttt ctttctccgt cagggacgaa
     4321 tgcccgggca gtttttgaag gtgttgttga acgaagattt tcgctcccgg gaagcggtaa
     4381 cgccgttatc attcagcatg gtaattatcg aacactatac ggaaacctga caaatattta
     4441 tgttcgtaca ggcgatcatg tgaaggcaaa acaagctatc ggacagatat ataccgatga
     4501 agaaaacgga ggaaaaacag aattgatttt ccagatctgg tctggtctga cccgcctaaa
     4561 tcccgaaaac tggataaccc gataatgata aaataaaccc tgacaatttt ttgatttaaa
     4621 tatggaaaat ttgatagaac aggaagttgc tgaaatcaga tatgatgatg ccaatatcgt
     4681 tacccttgaa ggtctggagc atatccgccg tcgtcccggg atgtacattg gaaaacttgg
     4741 tgatggtact cattccgatg atggaattta tgtccttctg aaggaggtca tcgacaactc
     4801 gattgatgaa tttcgtatgg gatatggaaa gacaattgag gttagtattg gcgagggcat
     4861 tgtgagtgtg agagattatg gacggggaat tccgctgaac agcctcgtaa aagcagtttc
     4921 ggtaatgaat accggaggga aattcgattc tcaggctttt aagaaatcag tcggtctgaa
     4981 tggagtcggt accaaggcag taaatgctct atcatcccgt tttgttattc agagttttag
     5041 ggaaggacaa tcgcgcagag ctgaatttga aaaaggttta ctacaaaccg aagaactgga
     5101 agaactgaaa gctgaaaatg gaacgctggt gatattcgaa cctgacaaag ctctgtttca
     5161 gaattatgtg tataattacg atattattga aacgatgctt cgtaattttt catatctcaa
     5221 taccggactt accattcttt tcaatgggaa aaaaatactt tcgaaaaatg gtttgaccga
     5281 cctgctcaat gagaacatga ccgccgaacc gctatatccc attattcacc tcaggactga
     5341 agatatcgag attgccttta cacacagcga ccagtacgga gaagaatact attctttcgt
     5401 caacgggcag catacaactc agggcggaac acaccagagc gcctttcgcg aagcggtggc
     5461 caaaaccatc aaggaatttt acaataaaaa tcatgaactt accgatatcc gtaacggaat
     5521 aatagccgca gtggctatcg gagtggaaga acccgtgttc gaatcgcaga ccaaaacaaa
     5581 acttggttca cgcgatatcg cgaaagatgg gataactgta aacaaattca tttccgattt
     5641 tgtaaaaaaa gaactggata actacctgca ccgcaatagt gaaacagcag aaattcttct
     5701 tcagaaaatt caggaatctg aaaaagaacg aaaagccatt gccggagtaa ctaaactggc
     5761 acgtgaacgg gctaaaaaag tgaatttgca caataaaaaa ttgcgcgatt gccgggtaca
     5821 ttacaacgat acaaaaggta gtcccgaact gctcgacagc agcagcattt ttattaccga
     5881 gggcgattcg gcaagcggct ccatcaccaa aagccgcgat gtgaatacac aggcggtgtt
     5941 cagccttcgt ggaaaaccac tcaacagctt cggactgacc aagaagattg tttatgaaaa
     6001 cgaagaattt aatttgctgc aggctgcgct caacatcgaa gatggtattg acgggctcag
     6061 atacaacaaa gtaattgtag ctaccgatgc tgatgtcgac ggaatgcaca tcaggcttct
     6121 gctcattacc ttttttctgc agttttttcc cgatttaatc aaaaaagggc atgtctttat
     6181 ccttcagaca ccacttttca gggtacggaa taaaaaagaa accgtttact gctattccga
     6241 agaggaacgg cttgctgcaa ttgcccggct gggagcaaat ccggaaatca cccgatttaa
     6301 aggacttgga gaaatttcac ccgacgaatt caaatatttc attggcaaag acattcgtct
     6361 cgatcaggtt acgcttaaaa aagaagatgc ggtgattgat ctgcttgcat tttatatggg
     6421 aaaaaatact tccgaacgtc agaatttcat tatagaaaat ctggtggtgg aagaagaggt
     6481 ggaataattt attttt
//
LOCUS       DAOPYB010000173         6490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_14573_length_6490_cov_9.683294, whole genome shotgun sequence.
ACCESSION   DAOPYB010000173 DAOPYB010000000
VERSION     DAOPYB010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14573_length_6490_cov_9.683294"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /locus_tag="PKH58_10980"
     CDS             <1..1947
                     /locus_tag="PKH58_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89599.1"
                     /translation="RVWQVRNTGNVGSVYFQIDLTAYPALPTAHFYALLVSTDANFTG
                     TTTYYKLTKGSGNLYSTQPVFASGSSYFTIAAIPNYWIGTQSTVWSNPANWSVGVPAT
                     GEDVEFATIANIGTPAVNDLIMDADYTIGNLVNVTNYKLVIPTTRTLTVNGTVNTNNA
                     NRILIRSAANTANGSLIFAQPTLNTSVLASVEMYSRAYKGDPLTFIDPVTGLTYTVSY
                     KWQYFGSPVKDAVYSSTFTADPISFVRVSDETKNGNTTYYDEWTQLYNEDKLVPFKGY
                     EITQNTDQSTGKLITFTGTLVTASQTLTLTNTPKGTAVDTGSGSNIFGNSFTAAIDIN
                     KIVFPASGVEKTVYLYNTGNLKEWSDYYDDAEGQLVAPGSYYAVPQNTSPVIQREIPS
                     MQGFMLTATTNNSTVTIPYSSVLNNTVPQRAPSLGGGSFSYLTVDVLSKTAADRVWLF
                     SHPFTSHRYDNGWDGRKISGTEGLSLFVDENYDQLQVSTTENLDSTFISLMAGKDTEY
                     TLRINKKYLTDYETLYLTDLVTKTVTDLSGLDEISYTFTAANTTKAERRFLITSKDSK
                     PNNELKPKQFDIFSRGNTVLIKNPSDASGVVRIFDMIGQLRHTTALKGNSLTEIKTAL
                     APAIYIVRVTATGIDQSEKIIIHH"
     gene            1960..2598
                     /locus_tag="PKH58_10985"
     CDS             1960..2598
                     /locus_tag="PKH58_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89600.1"
                     /translation="MKSIFLHILLITTVVVGAAAILTTNNPLYRKAEPAYITYKGGSG
                     EASSHVNLNGLNTSGRSFSGKSNSGKDDVSLAISSSRNTDDLTPIASSALTAGDKSLS
                     GSTTYAYRRTEKKNNSSGVAGFSGNEALGLLAQRGTGSDDENKEISGTSAVLAQTTYD
                     QAMPSDRYLAPTANKGKPAGGHPGLDPNLPIGDGVWILILLSVGYVIYKKKF"
     gene            3641..>6490
                     /locus_tag="PKH58_10990"
     CDS             3641..>6490
                     /locus_tag="PKH58_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89601.1"
                     /translation="MKKRMDDAFIKTLIHFTLPTDGKLNTGIYSGNRNSKLRTAIVAL
                     VFILSATVVAQAATYYSRASGAWNTNATWSTTGYGGAAATSFPTAGDIVNIGGGFTVT
                     VSSNVNCSTINIDAGSVLNPTGTRTVTATTGITINGTYTNQSTGTITTPSWICNGTYN
                     HATSSRNLPKGSTTSTWAANSNCNITGSYTTATQFANFIGQTFGNFTFNPLTMTSTVC
                     LYGDKSGSVTVQGNFTIVNTGSNTLYLRQSGFAYVGELNINGNLTMNTGIFDLHNGGS
                     IPTSSVVNLKGNLTLSCTSTMKQTTVQNGSFVKFNFIGTAIQKIDICSTASITSQATS
                     PTCAIQFIVASGATIDMGTSVLTGTNKTSFTLSDGAGIITANSGGLSTSGASGSIQVA
                     GSRTFSTTANYTYNGTTAQVTGNALTTANNLTFSNTSASGVTLSNAVNVTGTFAVTTG
                     AHVNLGTYTSKASSIVLGGIPRNAGLSYGGTGSTANVVDPTYFLANTGLLNVSLPAPT
                     NLSYNSPLSFRLNNVITAQSPTVTGLVDSYSITPALPSGLIFNTTTGVITGTPTVNSP
                     STTYTVTASNAAGSTSCEIVISVGNYFYAVASGNWNTAIWAPTSGGTADGQIPVSGDI
                     VFIGEASTNYTVTIPSGYAAACGSLTMGTYSDNTVATLNFTDASSSLTVGNDLVMNRP
                     NATATSVINVNAGSMTVGGTLKLANADLIPNNSTSLINQVNISTGTVTTKNLVFNGQS
                     DSQSQVIFSGPGTLNISGNLTLTYLLGTLTPSTGKVNFNGTTAQTIPVGMSKVTYNNL
                     TINNTSAAGATLSNAAISSANVTGNLTIGDINTGSTLDNGGYGITLASGKSLTVVNGS
                     TLRLSGTSTMATAPGGKVFGTTSTVDYCGSDQTVTAETYGHLILSGTGVKTMPTATTT
                     VAGNFTTAGTVSATAQAAINTAGNFTIGTGTTFNAS"
BASE COUNT         1975 a         1363 c         1318 g         1834 t
ORIGIN      
        1 cgtgtgtggc aggtaagaaa tacgggaaat gtgggttctg tttattttca gattgactta
       61 actgcttatc ctgcattgcc cacggcacac ttctacgctc tgctggtgtc aacagatgca
      121 aactttacgg gaacaaccac ctattacaaa ctaacaaaag gtagtgggaa tttgtactct
      181 acacaacccg tttttgcttc gggaagcagc tattttacta ttgctgccat ccccaattac
      241 tggataggta cacagagtac tgtctggagt aacccggcaa actggtcggt tggtgtgcct
      301 gctacaggtg aggatgtgga atttgctaca atagccaata tcggcactcc tgctgtgaac
      361 gatcttatca tggatgctga ttatacaata ggcaatttag tcaatgttac aaattataag
      421 ctggtgattc caactaccag gacgttgact gttaatggca ccgtcaatac caataacgcc
      481 aaccggatac ttatccgatc ggctgccaat acagccaatg ggagtcttat ttttgctcag
      541 cccacgctga atacttccgt tctggcttcg gtcgaaatgt attccagggc atacaaggga
      601 gatccgttaa catttattga tccggttaca ggtctgacat atacagtttc ctataaatgg
      661 cagtattttg gcagtccggt taaggacgct gtgtatagtt ccactttcac agccgatcct
      721 atatcgtttg tcagggtttc ggacgaaacg aaaaacggca ataccaccta ttacgacgaa
      781 tggacgcaac tttacaacga agataagctc gttcctttca agggttacga aattacccag
      841 aataccgatc agagtaccgg caaactgata actttcaccg gaacgctggt aacagccagt
      901 cagacattaa ccctgacaaa tactccaaaa ggtactgctg tcgataccgg ttcgggtagt
      961 aatattttcg gaaattcatt taccgccgct attgatatca ataaaatcgt tttcccggcg
     1021 agtggggttg aaaagaccgt ttacctttac aacaccggta atctgaaaga atggagcgat
     1081 tattacgacg atgctgaggg tcagctggtg gctcctggtt cttattatgc tgttccgcag
     1141 aacacttcac cggtcataca gcgcgaaata ccttccatgc agggttttat gcttacggct
     1201 acaacgaaca attccactgt taccatccct tactcctctg tgcttaacaa cacagttccg
     1261 caacgggcac cttcgttggg gggcggttcg ttttcgtatc tcacggtgga tgtactcagc
     1321 aagacggctg ccgaccgtgt atggcttttc agccatccat ttacatcgca tcggtacgac
     1381 aatggctggg acggacgtaa aatatcagga acggaaggtt tgagcctctt tgtggatgaa
     1441 aattacgatc agcttcaggt gagtactacc gaaaatctgg atagtacatt tatctcgctg
     1501 atggctggaa aggatactga atatacactg cgtataaaca aaaaatatct gactgattac
     1561 gaaacactct atctgaccga cctggtaacc aaaacagtca ccgatttgtc aggtttggat
     1621 gaaatcagct atacattcac cgctgccaat accacaaaag ctgaaagacg cttcctgatt
     1681 acatctaaag acagtaaacc taacaacgaa ttgaaaccga agcaatttga tatctttagc
     1741 aggggtaata cagttctgat aaaaaatccg tcggatgcca gtggagtagt ccggattttt
     1801 gacatgatcg gacagttacg acatactaca gcgttgaaag gaaatagcct gacagagata
     1861 aaaacagcat tagcaccggc aatctatatc gtgagagtta ctgccacggg tattgatcag
     1921 tctgaaaaaa taataattca ccattaagaa gagaacagtt tgaaaagtat atttttacat
     1981 attcttttaa taacaactgt cgtcgttggt gcagctgcaa tacttacgac caataatccg
     2041 ctttaccgta aagcagagcc tgcttacatt acctataaag gcggaagtgg tgaagcttcg
     2101 tcacatgtga atctgaatgg attgaataca tcgggaagaa gtttttccgg caaatcaaat
     2161 tcgggcaaag atgacgtttc gttagcaatc agcagttcgc ggaatacaga tgacttaaca
     2221 ccaattgcca gcagtgcatt aaccgcaggt gataaatccc tttcaggttc aacaacttat
     2281 gcataccgac gtacagaaaa gaaaaacaat tcttcgggtg ttgccggttt ttcgggtaat
     2341 gaagccctcg gactgctggc acaaagggga actggtagtg atgatgagaa taaggaaatt
     2401 tcaggtactt ctgctgtttt ggctcaaacc acttacgacc aggccatgcc atcagacaga
     2461 tacctggcac caacagcaaa caaaggaaag ccggccggtg gtcatccggg acttgatcca
     2521 aacctaccca taggcgacgg tgtctggatt ttaatactac tcagtgtggg atatgtcatt
     2581 tataaaaaga aattctgaaa agatactttc ctgtggcagt gaaatattaa gcatccggta
     2641 attgttgtcg gatttttttt tcgcccttcc ctctccgttt actaaggaaa ttaaactgtg
     2701 aacccactca caactgtcgg tagaatgtaa gtgtcattga tccctacatg gcaggtttct
     2761 aaatccgtaa ggtctgctat atattaataa ttcatgttcc tgaaacagaa cgaatagtta
     2821 atataacgtg agttcgatat aaaaatatgc agaacatctt caaaataagc taataaggtt
     2881 agatctatta taaattcatc ggctgctaaa gttgaaaaaa gtaaaaatta gtttattccc
     2941 tgaatttgaa cctccgaatg tgtctggata tttaaaaaat ccttcgtaaa aaattaaaag
     3001 tgcatttaat cttattgtca tattgttatt cagtagttta taagtgttaa taaataatct
     3061 tatgtcgaac tcacgttaac ataactccaa ccgagactat taatataaga tcatatatgt
     3121 tttggattat tgggtgaatc ttttccggat tgataaaata cactagtata ccacgatttc
     3181 ggtaattcct aaactcacaa ttattatatt ccaatataga aataaacaaa tactatttta
     3241 ttgtgttata tttatattat tgtatatttg tatacactaa ctgacctttt ttttattgct
     3301 cccaataaaa aagctaaatt tatctgttct gaaataaata attgactatt tgttcactta
     3361 gcttgttgat aggtggattt atattttgga ttactccatt tttttttatt ttgaaagaat
     3421 aaaaaactaa taacgtaatc aaaggaaacg attgtgtcaa ttcttctttt ttgtcttttt
     3481 ttcaaatatc atttttattg tattaaagtt tgtgttagcg cgaagatgat tttttaaatc
     3541 attaatactt tcaataagat cttttttcaa atgaaaattc agtaaagttt gaagtattta
     3601 ataaaattaa attatgagaa tgaataactt tttgtactct atgaaaaaaa gaatggatga
     3661 tgcttttata aagacattaa ttcatttcac tttaccgaca gatggtaaac tcaacaccgg
     3721 tatatattcg ggaaaccgaa acagcaaact tagaactgcc atagttgcgc tggtttttat
     3781 tttatccgct acagttgttg ctcaggcagc tacatactat tcaagagcca gtggagcgtg
     3841 gaatactaat gcaacctggt caacaaccgg ctatggtgga gcagctgcaa cgagttttcc
     3901 tacagctgga gatattgtca atatcggcgg tggatttaca gttactgtga gttctaatgt
     3961 aaattgttca acaataaata tagatgcagg ttcagtactt aatcccaccg gcacccgtac
     4021 tgtaaccgca accacaggta ttaccataaa cgggacttat acaaatcaaa gtaccggaac
     4081 cattaccaca ccctcctgga tttgtaacgg aacatacaat catgccactt catccagaaa
     4141 cctgcccaaa ggttcaacaa cttccacctg ggctgccaat tctaattgta atatcaccgg
     4201 atcttacacc acagccacac aatttgccaa tttcatagga caaacttttg gaaattttac
     4261 gtttaatcca ttgacaatga catccacagt atgtttgtat ggcgacaaat cggggtcggt
     4321 aactgtacag gggaatttta caattgtgaa tacgggatcc aacaccctgt acctccgaca
     4381 atccgggttt gcatatgtag gtgagttaaa tatcaacggc aatttaacca tgaataccgg
     4441 tattttcgat ttgcataacg gaggtagtat tccaacctca tccgttgtaa atctaaaagg
     4501 gaacctgaca ctatcgtgta caagtacaat gaaacaaaca acagttcaaa acggttcatt
     4561 cgtgaaattt aatttcattg gcactgctat tcaaaaaatc gatatatgtt ctacagcatc
     4621 tattacctca caagctacga gtccaacctg tgctattcaa ttcatagttg caagcggcgc
     4681 aaccatcgat atgggtacat ctgtacttac cggaaccaac aaaacttctt ttacgctgag
     4741 tgatggcgcc ggaattataa ccgctaattc aggcggtttg tcaacaagcg gagcttccgg
     4801 atcaattcag gttgcaggta gcagaacatt cagcacaaca gccaattata catacaatgg
     4861 aaccactgcg caggttacag gtaacgcgtt gactacagcc aataacttaa cgttcagcaa
     4921 taccagcgct tcaggtgtaa cactcagcaa cgcagtcaat gtgaccggaa cttttgctgt
     4981 tactaccggt gcccatgtca atctgggtac gtacacttcc aaagcatctt caattgtttt
     5041 aggaggtatt ccaaggaatg caggtctttc ttatggagga accggatcta ctgcgaatgt
     5101 agttgatcca acttattttc ttgccaatac gggtttgttg aatgtgagtt tgccagctcc
     5161 tacaaatctg agttataact cgcccttatc gttccggctg aataatgtaa taacggcaca
     5221 aagtccaact gtaactggtc tcgttgacag ctattcaatt acccccgcat taccgtccgg
     5281 tttaattttt aatacaacaa ccggtgtcat tacaggcaca cctacagtga attcaccttc
     5341 gactacttat acggttacgg caagcaatgc agcaggaagc acttcctgcg aaattgtgat
     5401 aagcgttgga aattattttt atgcagtggc aagcggtaac tggaatacgg ctatctgggc
     5461 tccaacttcc ggaggtactg ctgatgggca aattcccgtt tcaggtgata ttgttttcat
     5521 cggagaagcg tcaacgaatt atactgttac catcccatca ggatacgcgg ctgcctgcgg
     5581 aagcctgact atgggtacat acagtgataa tactgtagca acgctgaatt ttacggatgc
     5641 gtcaagctca ctaactgtag gtaatgacct ggtgatgaac cgcccgaatg cgactgctac
     5701 ctctgttatt aatgtgaatg ccggcagtat gacggtaggt ggaacactga aactggcaaa
     5761 tgcagattta attccgaata acagcacatc gctcattaat caggttaata tatcgactgg
     5821 tacggtaact accaagaatc tggtattcaa cggacagtca gactcgcaaa gccaggtgat
     5881 tttcagtggt ccgggtacgt tgaacatcag tggtaattta acacttactt atttgcttgg
     5941 aactttaact ccatctaccg gaaaggttaa tttcaatgga acaacggcac agacaattcc
     6001 tgttggcatg agtaaagtga cgtacaacaa tctcacgata aataacacaa gcgctgccgg
     6061 agctacttta agcaacgctg cgatatcctc tgccaatgtt accggaaatc tgacgattgg
     6121 cgatataaac accggaagta cgctggataa cggcggttat ggcataacgc tggcatcggg
     6181 caagtcattg actgttgtta atggttctac cctgcggttg tcgggaactt caacaatggc
     6241 aacagctccc ggtggtaagg tattcgggac aacaagtacg gtagattact gcggatcaga
     6301 ccaaaccgtt actgccgaga cttacggaca tctgatatta agtggaacgg gagttaaaac
     6361 aatgccaact gcaactacaa ctgttgccgg aaatttcaca acggcaggta cggtttcagc
     6421 tacagcacag gcagccatta acacagccgg gaatttcacc attggtacgg gaacaacctt
     6481 caacgcttcc
//
LOCUS       DAOPYB010000174         6356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_15037_length_6356_cov_8.954610, whole genome shotgun sequence.
ACCESSION   DAOPYB010000174 DAOPYB010000000
VERSION     DAOPYB010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15037_length_6356_cov_8.954610"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PKH58_10995"
     CDS             complement(<1..713)
                     /locus_tag="PKH58_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467877.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNX89602.1"
                     /translation="MKSERIIVISILSIALIIASAIAGRSVINRNIRNQTITVTGMAE
                     RNFVSDLIVWQSQFTVKKFNLTEAYAELKKQSETTRQFLLSKGVSKDEITFSAVEINR
                     DFSYTYGPNGNIASSTFDGFRLTQSVKINSKNVSKIETISREITELINQGIEITSLAP
                     EYYYTKLADLKIEMLAEASNDAYNRAETMAKNGKGKLGGMVDSSMGVFQIIAQNSSED
                     YSWGGSYNTTSKLKTATITV"
     gene            complement(756..2210)
                     /locus_tag="PKH58_11000"
     CDS             complement(756..2210)
                     /locus_tag="PKH58_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNX89603.1"
                     /translation="MKKALTIILFISLATQLSANYKKPVVTDNQRNDTVILPDLSKIT
                     LKDSIINYGKIYLSLPYRYGGTTPKGFDCSGFTSHVYGNFGINLNRSSREQASQFPEI
                     NKENLQTGDLVFFEGRRHNGVVGHVGIVTETKPNGEFNFIHSSIKKGVTISSSEEQYY
                     ASRYVKGGRVMVADNTLVKPSTKISLDFEKDNKPKYELVPKNKKQKLVEAKYHKVKKG
                     ETLSSIAEDYDVPVSTLKYLNGLKNSKIRRGKKLIISQAVYVSPEPTLDQLQTVASNK
                     VEETEPAKVVLHYASMSPTPSLEIKEPELKPVSKENIKLKDEPKPVIQLNNAASTTEF
                     YKVKAGESLYNIARTHNLTVDKLKEINNLKSNNITPGQLLAIKPAETLVAKTEQNHVI
                     KNASAKTKEVVMADAGITETKKHELKAEPATTEKKAEKQAIKKPESTVHVVKKGDTLY
                     SLSRQYGCKVAKIKEWNKDVDDNLQIGDKIKIVL"
     gene            complement(2335..3315)
                     /locus_tag="PKH58_11005"
     CDS             complement(2335..3315)
                     /locus_tag="PKH58_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX89604.1"
                     /translation="MSIVETNHLSIGYLKGKSRTVVQSELNLELNNGEMVCLIGPNGS
                     GKTTLLKTLAGLQKPLKGEVLINDRLISELSQQEKALEIALVLTDKVQVENATVRDIV
                     TLGQLPYTHWLGGLTDEGRKKVDEAIGMVHLKHKANYFLNELSDGERQRVMIAKALAQ
                     DTPLIFLDEPTAHLDLPNRVEIMLLLHRLAHKTQKAIILSTHELDLALQAADRIWLMS
                     ETDGVICGVPEDLVIDGSFNRIFQSDAYYFNASNGNFSMNYRLTKKLSLTGDNTRLYW
                     TLRALARSGFYVTDDAETHIDITDKGWELNGKIHPDIETLLNELAGFTEI"
     gene            complement(3318..4313)
                     /locus_tag="PKH58_11010"
     CDS             complement(3318..4313)
                     /locus_tag="PKH58_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNX89605.1"
                     /translation="MKHRKTNLFILLTILLVILFLADLTLGSIRIPLKDMINVFLGNS
                     TNNINSEILLNFRLPKAITAILTGAALSVAGLLMQTLFRNPLADPYILGVSSGASLGV
                     ALVMMASALLPVIFISSGWGLIIAAIIGAALVLLLVVGVSFKVQNSVSLLIVGLMFGT
                     MASSLVSIMQFYSNPDAVKLFVMWNMGSLSAVTWNYMYVLFPVILTGLLIAFLLTKRL
                     NGLLLGENYARGLGIPIIQTRILIVIATGILAGSVTAFTGPIAFVGVAVPHIARGLFR
                     SARHQHLLPACILSGASLLLICDIISQIPAHALPVNIISALFGAPVIIWIILKRK"
     gene            complement(4310..4867)
                     /locus_tag="PKH58_11015"
     CDS             complement(4310..4867)
                     /locus_tag="PKH58_11015"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNX89606.1"
                     /translation="MTELSINEDIKLKQLEESDAPDIFTMIDSQREYMGKWLPFVAET
                     KTVDDSLAYIRMIAETPESFRELVFCILYRGKFAGLIGLKFNPADKPNKRTEIGYWLS
                     ENLQKKGIMTLSAKALTDYAFNVLDLNRVAIKCATGNLNSRNVALRLGFTYEGTERDG
                     ELFPDNHFVNLEVYSMLKKDWKQLK"
     gene            complement(4890..6017)
                     /locus_tag="PKH58_11020"
     CDS             complement(4890..6017)
                     /locus_tag="PKH58_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX89607.1"
                     /translation="MKKILLIIIGICVLVSCNNKSSNNKTTKQPLNQKLLNFATGFAI
                     NNFENYKEIVVYSPWKKGETYARYYLVKDEKITTPNDGFKIKIPLKTLATTSVTHLEF
                     LKLIGELQTISGVCSPDLIYNPEIRKNVQHAKISDLGDAFSLNVEKVMLLKPGALMTS
                     GFNQTDANAQRIQQAGIPVIFNNEWMETSLLGRAEWIKFVAAFFDRETVADTIFAGIE
                     KRYNKLKSRIADVPDKPKIVSGNNFRGTWYMPAGSSFMGNLFKDAGASYYYANDSTTG
                     SLPLNMETVLQHFADTDIWLNANFSTISELIKSDSKHALFRPVTNGQVYNFNKRLLPG
                     SANDFWESAVARPDLLLADVIAILHPDLMPGYQLVYAEKLK"
     gene            complement(6161..>6356)
                     /locus_tag="PKH58_11025"
     CDS             complement(6161..>6356)
                     /locus_tag="PKH58_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89608.1"
                     /translation="VFNLAVVLPQMMSNGVANVIKQTGNYNILYIACGLFIATSIVFW
                     IFVREPESSNTKETIPSGGH"
BASE COUNT         1646 a         1307 c         1362 g         2041 t
ORIGIN      
        1 acggtaatcg tagcggtttt aagttttgaa gtggtgttgt aagagccacc ccagctgtaa
       61 tcctccgatg aattttgggc gataatctga aatacgccca tactcgaatc taccataccg
      121 cccagttttc cttttccgtt tttagccatc gtttccgcgc ggttatacgc gtcgttggag
      181 gcttcggcaa gcatttctat tttgaggtcg gcgagtttgg tgtaatagta ttcgggagcc
      241 agtgaggtta tttctattcc ctgattgatc aactcagtga tttccctcga gatggtttca
      301 atttttgaaa cgttttttga gtttatcttc acgctttgtg tcagccggaa accatcgaaa
      361 gtggatgaag cgatattccc gttgggaccg taggtgtagc tgaagtcgcg gttaatctcc
      421 acggccgaaa aagtaatttc atccttgctg acacccttgg ataataaaaa ctgacgggtg
      481 gtttcacttt gctttttgag ttcggcataa gcttcggtca ggttaaactt tttgacagta
      541 aactgacttt gccagacaat aaggtcagat acaaaattgc gttcagccat acctgtaacc
      601 gttattgttt ggtttcgtat gtttcggtta attacagagc gtcctgcaat ggcactggca
      661 ataattagag ctatggaaag tatcgaaatt acaataattc gttctgattt catggttttg
      721 tgtttttgtg gttaatattt tatttataaa aacgcttaaa gaacaatttt tattttgtca
      781 ccaatctgta gattatcatc cacatcttta ttccattctt taatttttgc tactttacaa
      841 ccgtattgcc tcgacaggga gtaaagggta tcaccttttt ttacaacgtg aacagtgctt
      901 tcaggttttt tgatggcttg tttttccgcc ttcttttcgg tggtggcagg ttcggctttt
      961 agctcatgtt tcttcgtttc tgttatcccg gcatcagcca tcaccacttc ctttgtttta
     1021 gcagaggcat tcttgattac atgattttgt tcagtttttg caactaaggt ttctgctggt
     1081 tttatggcaa gcaactgtcc cggtgtaatg ttattcgatt tcaggttgtt gatttctttc
     1141 agcttgtcaa cagtcagatt atgagtacgg gctatgttgt agagtgattc tccggcttta
     1201 accttataaa attctgtggt tgatgcagcg ttattcagtt gaatgacagg ttttggttcg
     1261 tctttcagtt tgatattttc tttggatacc ggttttagct caggttcttt gatttcaagt
     1321 gaaggtgttg gcgacatgct ggcataatga agtactactt tggccggttc tgtttcttca
     1381 accttattcg acgcgacagt ttgcagttgg tcgagtgttg gctccggcga aacatataca
     1441 gcctgactga tgataagttt cttaccacgt ctgattttgc tgtttttcag tccgttcagg
     1501 tatttaaggg tgctaaccgg cacatcataa tcttccgcaa ttgacgaaag tgtttcgcct
     1561 ttcttcacct tatggtattt ggcctcaaca agcttttgct ttttattttt gggaaccagt
     1621 tcatacttag gcttattgtc tttttcgaaa tcgagtgaga ttttcgttga tggtttgacg
     1681 agcgtattgt ctgccaccat cactctgcct ccttttacat agcgtgaagc ataatactgc
     1741 tcctctgagg aagatattgt tacacctttt tttatggatg aatggataaa attaaattct
     1801 ccgtttggct tggtttcagt tactattccc acgtgaccta ccacaccatt atgacgtcgt
     1861 ccttcaaaaa aaaccaggtc acctgtttgc aaattttctt tatttatttc tggaaactgt
     1921 gacgcctgtt cgcgggaact acggttgaga ttaataccga agttaccata gacatgagag
     1981 gtaaatcccg agcagtcgaa acctttgggt gtggtaccgc cataacggta tggtaatgac
     2041 aaatatattt ttccgtagtt aatgatcgaa tctttcagcg tgattttgct taaatcggga
     2101 agtataaccg tatcgtttct ttgattatct gtaacgacag gttttttata atttgcagat
     2161 aattgagtgg ctaaacttat gaatagtatt attgtgaggg cttttttcat tctaaaattt
     2221 tatcagaaat tgaatttgac aacgcgaatg atgattcgga tactttacaa aaatactttt
     2281 tataattcag aaaaaagatt gccctttttc tttttaaaca aaattagaat ccgtttagat
     2341 ttcagtaaat ccggccagtt cgttcaataa ggtttcgata tcaggatgga tttttccgtt
     2401 cagttcccag cctttgtcgg ttatatcaat atgtgtttcg gcatcatcag tgacataaaa
     2461 tcccgaacgg gccagggcgc gcaatgtcca gtacaaacgg gtgttgtcgc ctgtaagact
     2521 tagttttttt gtcaggcgat agttcatgga gaaatttccg tttgaagcat tgaaatagta
     2581 agcatcgctc tgaaagatgc ggttgaagct tccgtcaatg acgagatctt cgggtactcc
     2641 gcagattaca ccgtccgttt cactcatcag ccagatacgg tcggctgcct gtagtgccag
     2701 gtcgagttcg tgtgtggata ggatgatggc tttttgcgtc ttgtgtgcca gccggtggag
     2761 caggagcatg atttccaccc ggttgggcag gtcgagatgc gcggttggtt catccagaaa
     2821 aatgagtgga gtatcctgcg ccagagcctt ggcaatcatc acacgttgtc gttcaccgtc
     2881 ggagagctca ttcagaaaat aattggcttt atgcttgaga tgcaccattc cgatggcttc
     2941 gtcaaccttt ttgcggcctt cgtcggtcag tccgcccagc cagtgggtgt acggaagttg
     3001 tcccagtgtt acgatatccc tcacagttgc gttttcaacc tgcaccttgt ctgtgagtac
     3061 cagtgcgatt tcaagcgctt tttcctgttg cgaaagttcg gaaatcagtc tgtcattaat
     3121 caatacctct cccttgagcg gtttttgcaa tccggcgagt gttttgagca aggttgtttt
     3181 tccgctgcca ttgggtccga tgagacatac catttcacca ttgttcagtt ccagattcaa
     3241 ttcgctttgc acgactgttc tggattttcc tttaaggtat ccgatggata aatggttggt
     3301 ttcgacgatg gacataacta ttttctttta agtattatcc agataatgac cggcgcgccg
     3361 aacagggcgc tgatgatatt aaccggcagc gcgtgagccg gaatttgtga tatgatatcg
     3421 caaatcaaca ggagtgaagc tcctgataaa atacatgccg gtaataagtg ctgatgcctg
     3481 gccgatcgaa acaatccgcg tgcaatgtgg ggaacagcca ctcccacaaa cgcgatggga
     3541 ccggtaaatg cagttactga ccccgctaaa atacccgtag cgatcactat cagtatgcgg
     3601 gtttgaatga tgggaattcc aaggccgcgc gcatagttct cccccagcaa aagaccgttc
     3661 aaccttttag taagcagaaa tgcgattagt aaacctgtca gtattacagg gaataataca
     3721 tacatataat tccaggttac ggcgctcagg cttcccatat tccacattac gaataacttg
     3781 acagcatctg gattgctata aaactgcatg atgcttacca gcgatgaagc catggtgcca
     3841 aacatcagtc ccacgataag cagcgacacc gagttctgaa ctttaaacga cactcctaca
     3901 accagcaaca gtaccagagc cgctccgatt atggcagcaa ttatcaatcc ccaaccactt
     3961 gatatgaata taacaggtaa aagggcagaa gccatcatca ccagggctac tcccagactt
     4021 gcccccgaac ttacacccag gatgtatggg tcggccaggg gattgcgaaa aagtgtttgc
     4081 atcaatagtc cggcaacaga aagcgctgca ccggtaagga tagccgtaat tgctttgggt
     4141 aatctgaaat tcagaagaat ttcagaattg atattattcg tgctgtttcc cagaaaaaca
     4201 tttatcatgt ctttgagtgg tattcgtata cttcccaaag tgagatctgc cagaaataaa
     4261 atcaccagca gtatcgtcag caagataaat aagttcgttt tccggtgttt catttaagtt
     4321 gtttccagtc ttttttcaac atgctgtaaa cctcaagatt gacaaagtga ttgtcgggga
     4381 aaagctcacc atctcgttcg gtaccttcgt aggtaaagcc aagccttaaa gctacgtttc
     4441 tgctgttcag attgccggtg gcgcatttaa tcgccacacg gttaaggtcc aacacattga
     4501 aggcataatc cgtaagagcc ttggctgaaa gggtcattat tcctttcttt tgcagatttt
     4561 ccgaaagcca gtatccaatc tcggtacgtt tattcggttt atcggcagga ttgaacttca
     4621 agccgataag acctgcaaat ttaccacgat atagtatgca aaaaaccagt tcgcgaaaac
     4681 tttcgggtgt ttcagctatc atgcggatat aagcaaggct gtcatcaacc gttttggttt
     4741 cggcaacaaa cggaagccat ttgcccatat attcacgctg actgtcaatc atagtaaaaa
     4801 tatcgggagc atcactctct tcaagctgct ttagttttat gtcttcgttt atagacagtt
     4861 ctgtcattgc ttaatagtct tgataataat tatttcagtt tttctgcata aacaagctgg
     4921 taaccgggca taagatcggg atgcagaatg gcaatcacat cggccagcaa caagtcgggt
     4981 cgggcaactg cgctttccca aaagtcattg gcactgccag gcagcaaacg cttgttgaaa
     5041 ttatatacct gtccgtttgt aacgggacgg aatagcgcgt gtttactgtc cgattttatc
     5101 agttcactga ttgtgctgaa gttggcatta agccagatgt ccgtatccgc aaaatgctgg
     5161 agaacagttt ccatgttcag tgggaggctg cctgtggtgc tatcgttggc gtaataatat
     5221 gatgcaccgg catctttaaa caggttgccc ataaaactgc tgcccgcagg catgtaccag
     5281 gttcccctga agttattgcc cgacactatt tttggtttat caggaacatc agcaattctg
     5341 gatttcaact tgttataccg cttctcgata cctgcaaaaa tggtatcagc tacagtttcc
     5401 ctgtcaaaaa atgcagcgac aaatttgatc cattccgccc gtccaagcag cgatgtttcc
     5461 atccattcat tattaaatat gacgggaata ccggcttgct gaatgcgctg tgcgttggca
     5521 tcggtctggt tgaagccgct cgtcattagg gcgccgggtt taagcagcat tactttctcc
     5581 acattcagac tgaacgcatc acccaaatca gatattttag catgctgtac atttttccga
     5641 atttcgggat tatatatcag atcaggactg caaactccgg aaatggtttg taattcacca
     5701 attaatttca aaaactcaag atgtgttaca gaagtcgtgg caagagtttt cagaggtatc
     5761 tttattttaa atccatcatt gggtgtagtt attttctcat cttttaccaa ataataacgg
     5821 gcgtatgttt ctcctttttt ccatggacta tacactacga tttctttata attttcgaaa
     5881 ttattgatag caaatcctgt agcgaagttt aataattttt gattaagggg ttgtttggtt
     5941 gttttgttgt ttgatgattt gttattgcac gatactaaga cacaaatacc gattattatg
     6001 agtaaaatct ttttcatggt tatggttttt tactccacaa agataagcag aaatattata
     6061 cttgaggaat ataaaaatcc tttttcgatt taacgaaaaa ggattagtat tttaatatct
     6121 gttaattttt ctggttttta cagtttaact caggaactta tcaatgccca cccgaaggaa
     6181 tggtttcttt tgtatttgat gattcgggtt cgcgaacaaa aatccagaat acaatagaag
     6241 ttgcaatgaa caacccgcag gcaatgtata agatgttgta gttaccggtt tgtttaataa
     6301 catttgccac accgttactc atcatttgag gaagcactac tgccaaatta aatacg
//
LOCUS       DAOPYB010000175         6252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_15415_length_6252_cov_6.627400, whole genome shotgun sequence.
ACCESSION   DAOPYB010000175 DAOPYB010000000
VERSION     DAOPYB010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15415_length_6252_cov_6.627400"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="PKH58_11030"
     CDS             <1..1523
                     /locus_tag="PKH58_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89609.1"
                     /translation="GLITGTKFTKTGTGTLYIGSNNPNLTETILTAGTIKLNSDASIA
                     YGVGKKITLLTGTLDSKNSTGAYQTSSHNIEVPAGSTAAVIAAPRCQYNGVLTGGGTL
                     NWKTDYIRAYMNANWSDFSGNINITANSANSTYEDHFIVNNVNGYPKATVNLGSGIRM
                     CYKNGTSDNGRTKIKTGMLTGVAGSEFYNADLEVGATNASGSFDGVIMGSTVVTKIGT
                     GTWTLGGANTYTGNTNLNGGTLVLNGSLTGTGTFTVGTATFFNVKGAVAGPVVIALNG
                     IASLTGTLNSTLTSRGILRGTGGIVKGNVFLNDSSVTQPGSASIGSFTFGNTTMSSGA
                     VLEMQLIGSTTSCDLLNVTGTLTLGGTLNLSGSGVFKSTDTYKLFNATTLTGTFARII
                     LPVLQNGLEWDLTELYTNGIVKVKLNETGIRNPTIRIGVVANPTDGLFKILVGNQTDF
                     NLQVVDLQGKIVYKSVLNSTRSEYDLDIRKSPAGIYLLNITSQKGYSNTLKLLKNR"
     gene            complement(1715..2596)
                     /locus_tag="PKH58_11035"
     CDS             complement(1715..2596)
                     /locus_tag="PKH58_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX89610.1"
                     /translation="MRFNDFAIDFKYLLVNERDKKFGLTVNTVGFQPIAPNTLYPSTD
                     HPKKYFFDPCKGRILPEFQFVYISKGKGVFSSESTKRQTVKKGQIIIIFPGQWHTYCP
                     DPESGWNEYYIGFEGQIINSYVQNGFISPENQVLDVGFNEDLVHLFSTAIKVAKDEKK
                     SSQQYLSGIVFHILGMILSFSENKSLESNQYSQVIERAKMIMFENVKNVIDIQELAGN
                     LGTSYSWFRKVFKEHTGYSPAHYFQELKLRKAKELLAETTIPIKEIAYELNFSSYGYF
                     LSFFKKRVGCTPFEYRT"
     gene            complement(2657..3688)
                     /gene="rhaT"
                     /locus_tag="PKH58_11040"
     CDS             complement(2657..3688)
                     /gene="rhaT"
                     /locus_tag="PKH58_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HNX89611.1"
                     /translation="MNTLFGLLIIAIGSMGQSSSYVPIKKVKSWAWESFWLVQGVFAW
                     LIFPFLGALLAIPDGHTLGEILSSGDGAAWKAMFFGALWGVGGLTFGLSMRYLGVALG
                     QSIALGTCSAFGTLIPALMSGTDLLHGKGLILLIGVSVAIAGISVIGYAGSLRAKNMT
                     DEQKKAAIKDFALGKGLLVALLAGVMSACFSLGLEAAEPLRAAAVSMGTKALYAGLPA
                     ILMITIGGFITNAVYCIFQNFKNKTLKDYHTIPGKVLINNLLFCALAGGLWYSQFFGL
                     ALGKSFLTEGSAMMAFSWSILMSLNVTFSNVWGIILKEWKGAGKKAIVYLVIGMLILI
                     FSLIIPGIF"
     gene            complement(3813..5075)
                     /locus_tag="PKH58_11045"
     CDS             complement(3813..5075)
                     /locus_tag="PKH58_11045"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HNX89612.1"
                     /translation="MKEEQILRAYLDAKEQYATLGIDTDKALEQLDKLPISLHCWQAD
                     DVTGFENPDGVLTGGIQTTGNYPGKARTISEVRADIDKAMGLIPGNQRVNVHAFYGDF
                     GGQLVDRDQIEPSHFQSWIDWAKEKNYKLDFNCTCFSHDKSADGLTLSHPDPEIRNFW
                     IEHVIRCRKIAEEMGRQLGSKCMHNIWIPDGSKDVTVNRMQYRKNLKESLDKIFEYKT
                     NDEYMLDCIESKLFGIGAESYTVGSHEFYMGYGMKNNIVVTLDSGHYHPTEVISDKIS
                     SLLLFLPEVMLHVSRGIRWDSDHVVILNEELLAIAQEIVRADAVEKVHVGLDYFDASI
                     NRIGAYVVGVRATQKALLQALLEPIAKLREYESNGQNFERMALLEEAKSMPWGAIYNY
                     YCAQKGVIVGEAYIADIQKYEKEVTLKR"
     gene            complement(5101..>6252)
                     /locus_tag="PKH58_11050"
     CDS             complement(5101..>6252)
                     /locus_tag="PKH58_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNX89613.1"
                     /translation="PERFFAKMAKEEVYRKTGIQIMNFNSLFQLSTQFEEDNSIYPLT
                     DKILFIPDALSYLLTGHMVTEYTIASTSQMLNPYLREFDTSLLEINHLSPDKFAPIVT
                     SGTVIGQLSESVSRQTGMKDISVIAVAGHDTASAVLATPAENENFAYLSSGTWSLMGI
                     ESKVPVINEKTYALNFTNEGGSDGSIRLLKNICGMWLIEQCKKDWEINGTVTYQEIVQ
                     AAEASVPFRCFINPDSPCFASPQSMISAIQDYCRQTGQYIPETMGEIARCIYESLAYR
                     YKQVLSNLQNLASFPINTLHIIGGGSQNKMLNSFTANAIGKPVIAGPSEATAIGNILL
                     QAQAAGVVKDKNEIRQIIRHSFEPETIEPQNVSLWEENYNNYLKVYKEL"
BASE COUNT         1865 a         1286 c         1290 g         1811 t
ORIGIN      
        1 atggattaat taccggaaca aaatttacca aaacaggaac cggaactttg tatataggca
       61 gcaacaaccc aaatttgact gaaactattt taactgccgg aactattaag ttaaattcag
      121 atgcctctat tgcttatggt gtaggtaaaa aaattacact gctgacaggt acactggata
      181 gtaaaaatag tactggcgct tatcagacaa gcagtcacaa catagaagtg ccggcaggat
      241 ctaccgcagc cgtaatagct gctccccggt gtcaatacaa tggagtttta accggtggtg
      301 gcactctcaa ctggaaaaca gattacattc gtgcttatat gaatgcaaac tggtctgatt
      361 tttcgggaaa tataaacatt acggctaaca gtgcaaactc aacgtatgag gatcacttta
      421 tagttaataa tgtaaatgga tacccgaaag caacggttaa tctcggttca ggaatacgta
      481 tgtgctataa aaacggcacc agcgacaacg gacgaacaaa aataaaaacc ggtatgttga
      541 caggtgttgc cggttcggaa ttttacaatg ccgacctgga agtgggagcc accaacgcga
      601 gtggttcatt tgatggtgta atcatgggct caacggtggt cacaaaaatc ggaacaggaa
      661 catggacact gggcggagca aatacgtata caggaaatac aaatcttaat ggcggaacat
      721 tagttcttaa cggaagcctt acaggtacag gtacatttac agtaggtaca gctacatttt
      781 tcaatgtaaa aggagcggta gccggaccgg tagtaattgc tctcaacggt attgcgtcat
      841 taaccggtac attaaattct acgttgacat cacgcggcat attgcgtggc acagggggaa
      901 ttgtaaaggg aaatgttttt ctaaatgata gttctgtcac acagccggga tcggcctcaa
      961 taggaagttt cacttttggg aataccacta tgagttcggg agccgtgctc gaaatgcagc
     1021 taattggcag tactacatcg tgtgacttgt tgaatgttac cggcacgcta accctgggcg
     1081 gtactttaaa tctctccgga agtggggttt ttaaatcaac tgatacttac aaactgttta
     1141 atgccacaac gctgaccggc acatttgcta gaatcatact acccgtatta caaaacggac
     1201 ttgagtggga tctgacagaa ctctatacga acggaattgt aaaagtcaaa ttaaatgaaa
     1261 ccggcattag aaatccaaca atcaggattg gagtagtagc taatccgacc gatggtctgt
     1321 tcaaaattct cgtaggcaat caaactgatt tcaacttaca ggttgtcgat ttacagggaa
     1381 aaatagtgta taaatctgtg ttgaattcta caagaagtga gtatgattta gatattagga
     1441 aaagtcctgc tggtatttat ctcctgaata tcacctcaca gaagggctat tcaaatacac
     1501 taaaattgct gaaaaacaga tagaataaaa tttcaacaaa ataaaagcag tgaatagaga
     1561 attctaacca ctgctttttt ttatctgtga aatcctgaat atttcatcat aatcaaccgg
     1621 aatttgtaac ctttacgaat cggaaagtga cttacttacc acccgatttt aataaagtct
     1681 gcgatatttt attttttaga ataaacggtc aaaattaagt ccgatattca aaaggagtac
     1741 atcctactct ttttttaaag aaagaaagaa agtaaccgta cgaactgaag ttgagttcat
     1801 aagcaatttc cttgattgga attgttgtct cagcgagaag ttctttggct tttctgagtt
     1861 taagttcctg aaaataatgt gcaggagaat acccggtgtg ttctttgaaa actttcctga
     1921 accacgagta acttgtaccg aggtttccgg cgagctcctg aatgtcaata acatttttga
     1981 cattttcaaa catgatcatt tttgctctct ctatgacctg agagtattga ttggattcca
     2041 aactcttatt ctcagaaaaa gataaaatca tacccagaat atgaaaaaca attcccgaaa
     2101 ggtattgttg tgatgatttt ttttcatctt tagccacttt tattgccgta gaaaacaaat
     2161 gtacaaggtc ttcgttaaaa cctacatcca aaacctgatt ttcaggtgat atgaagccat
     2221 tttgaacgta ggaattgatg atttgacctt caaatccaat gtagtattcg ttccagcctg
     2281 attcgggatc ggggcaatag gtatgccatt gccccgggaa tatgataatg atttgacctt
     2341 tttttacagt ttgtctttta gtagactcgg aggaaaatac acctttccct ttgctgatat
     2401 aaacaaattg gaattccggt aaaatccgcc ccttgcaggg atcaaaaaaa tattttttag
     2461 gatgatcagt tgaaggataa agggtgttgg gagcaatagg ctgaaaacca acagtattga
     2521 ctgtcagtcc aaacttttta tcccgttcat tgactaacaa atatttgaag tcaatggcaa
     2581 agtcgttaaa tctcatgact gatttttttg tgttatttca ggtattgagc tattaacgac
     2641 aaaattaaaa aaaaaatcag aatatgccgg gaattatcaa ggaaaatatg agtattaaca
     2701 taccaattac cagataaact atagcttttt taccggctcc tttccattct ttcagtatta
     2761 ttccccatac attgctgaaa gtcacattca gcgacataag gatactccac gaaaatgcca
     2821 tcatggcgct gccttctgtc agaaaacttt taccgagagc taatccgaaa aactgtgaat
     2881 accacaaccc ccccgctaaa gcacagaata aaagattgtt tatcaatact ttgccgggta
     2941 tggtgtgata atctttaagg gttttgtttt tgaaattctg aaagatacaa taaacggcat
     3001 tggtaataaa tccacctatc gtaatcatca gtatggccgg cagtccggca tacaatgcct
     3061 tggttcccat acttaccgct gctgctctga gtggctctgc agcctccagt ccaagactga
     3121 aacaggcact catcactccg gccaaaagcg ccaccaacaa gcctttgccc agagcaaaat
     3181 ccttgatagc agctttcttt tgctcgtcgg tcatgttttt ggcacgaaga cttccggcat
     3241 atccgataac tgaaatgccg gctatagcca cactaacgcc gattaacaga atcaatcctt
     3301 ttccatgcag caaatcagta cctgacatca atgccggaat aagcgttccg aaagcggagc
     3361 aggtgccaag ggcaatactc tgtcccagtg caacacccag atagcgcatg ctcaaaccaa
     3421 aggtaagacc tccaactccc cagagtgctc cgaagaacat tgctttccag gctgcaccat
     3481 cacctgatga aagaatttcg cccagtgtat gtccgtcggg tatagccaat agtgctccca
     3541 gaaatggaaa aatcagccag gcaaatacgc cctgaacgag ccagaaactc tcccaggccc
     3601 agcttttaac ttttttgatg ggaacgtatg agcttgactg gcccatactg ccgattgcaa
     3661 ttatcaataa tccgaagagt gtattcataa atattaagcc caccctaacc atcccaggga
     3721 ggagggaatt agtggttgat aaagttaata aaaatcagaa tttatatttt aaaaacaaaa
     3781 tcccctgatg gggggatttt gatggccttt ttttatcttt tcagtgttac ttctttttcg
     3841 tatttctgaa tatcggcaat gtaggcttct cccactatca cacctttttg agcacagtag
     3901 tagttgtaaa ttgcacccca gggcattgat ttagcttctt cgagcagtgc catgcgttca
     3961 aagttctgac cattgctctc gtattcccgc aatttagcta ttggctccag taatgcctgt
     4021 agtaatgctt tctgagtagc acgaactcct accacgtaag ctccgatacg attgatggaa
     4081 gcatcaaaat aatcaagacc aacatgaact ttttcaacag catctgcccg aacgatttcc
     4141 tgagcaatgg caagtaactc ttcattgaga attacaacgt ggtcgctgtc ccaacgaatg
     4201 ccacgggata cgtgaagcat cacttcgggc agaaatagca ataacgaaga aattttatcg
     4261 gaaatgactt cggtggggtg gtagtgacct gagtcgaggg taacgacaat gttgtttttc
     4321 atgccgtatc ccatatagaa ctcgtgagaa ccaacagtat aactttcggc accaataccg
     4381 aaaagtttac tttcaataca atcaagcata tattcatcgt tggttttgta ttcgaatatt
     4441 ttatccagcg attctttcag atttttacgg tactgcatac ggttgacagt gacatctttt
     4501 gagccgtcgg gaatccaaat gttgtgcata cattttgaac ccagttggcg tcccatttct
     4561 tcagctattt tgcggcagcg gattacgtgc tctatccaga aattacggat ttcgggatcg
     4621 ggatgagaaa gtgtaagacc atcggctgat ttatcatgtg aaaaacaggt acagttaaag
     4681 tcaagtttgt aattcttttc tttcgcccag tcaatccagc tttgaaaatg tgaaggttca
     4741 atttgatcac gatcaaccaa ttgaccgcca aaatcaccat aaaaagcatg tacatttacc
     4801 cgctgattac cgggaattaa gcccatggct ttatcaatgt cggcacgaac ttcgcttatg
     4861 gtgcgtgctt tgccgggata gttgcctgta gtctgaatac ctccggtgag tactccgtcg
     4921 ggattttcaa aaccggttac gtcgtctgcc tgccaacagt gtagtgagat aggaagttta
     4981 tccaattgct ctaatgcttt atcagtatca atgcccaggg ttgcgtattg ttcttttgca
     5041 tccagatagg ctcttaaaat ctgttcttct ttcatgattt tagtgctgtt tttttaatat
     5101 ttataattct ttatatactt tcagataatt gttataattt tcttcccata atgagacatt
     5161 ttgaggttca atggtttccg gttcgaatga atggcggata atctgtctga tttcattctt
     5221 atccttaacc actccggcag cctgagcctg aagcagtata ttcccgatag cagtggcttc
     5281 cgatgggccg gcaatgaccg gtttaccgat ggcgtttgct gtaaatgaat tcagcatttt
     5341 attctgtgaa cctccgccta taatgtgcag tgtgttgata gggaaactgg caagattttg
     5401 aagattagat aatacctgtt tatatcggta agccagactt tcatatatgc accgggctat
     5461 ttcacccatt gtttcgggaa tatattgacc ggtttgtctg cagtaatcct gaatggcact
     5521 aatcatcgat tgtggacttg caaagcaagg cgagtcgggg tttatgaagc accgaaaagg
     5581 cacagaagcc tcagcagcct gtacaatttc ctgataagtt accgttccgt ttatttccca
     5641 atcctttttg cactgctcaa tgagccacat accgcagata tttttcaaca gccggatgga
     5701 accatcggaa ccgccttcat tggtgaaatt gagagcatag gttttttcat tgatgaccgg
     5761 cactttcgat tcgataccca tcagcgacca ggtacccgaa ctcaggtagg caaaattctc
     5821 gttctcagca ggtgtggcta gcactgcaga ggcggtgtca tgtcctgcaa cggcaattac
     5881 cgaaatgtcc ttcattcccg tctggcgcga aacggattcg gataattggc cgattacagt
     5941 tccggatgtt acgatagggg caaatttatc cggactaaga tgatttattt cgagcaagga
     6001 agtgtcgaac tctctcaagt aaggattcaa catctgcgaa gtggaagcaa tggtgtattc
     6061 ggtaaccata tgacctgtaa gcaaatatga taaggcgtcg ggaatgaaaa gtatcttatc
     6121 agttaacgga tatatggagt tgtcttcttc aaattgtgtg gaaagttgaa aaagactgtt
     6181 gaaattcata atctgaatgc ccgtttttcg gtaaacctct tctttggcca ttttggcaaa
     6241 aaatctttcc gg
//
LOCUS       DAOPYB010000176         6235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_15486_length_6235_cov_10.587702, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000176 DAOPYB010000000
VERSION     DAOPYB010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15486_length_6235_cov_10.587702"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1046
                     /locus_tag="PKH58_11055"
     CDS             207..1046
                     /locus_tag="PKH58_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89614.1"
                     /translation="MKYKHFALVTLMLIFAILIKAQQPWRFHIAFEDGSGQRDTLWMI
                     FDNNATIGIDTLLGELTPQFDSTEFNVFMQSYDTIYTKVRAYPYSLYPYMTTGSIYSI
                     NYTYPVIIRWDTALFHAPYLPTSPTMNIAIIVGNYPFFMLPIDPTAPYLHTINMLTKD
                     SLFCPEDLNGVQFPLFGDQSLGFAVDHDNYLYLVEKKTERVLLYPNPANEYLLVSMEN
                     PVISFCITDMTGNIVISEYFNSYQKMEAYKINCQDLCAGLYLIEITNFKNQKHYEKFI
                     ITK"
     gene            1021..5907
                     /locus_tag="PKH58_11060"
     CDS             1021..5907
                     /locus_tag="PKH58_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89615.1"
                     /translation="MKNLSLLNKKLLCVIAIMALTSTLLGQVCLTGSIESEPSDNPNT
                     VYASRFGSYLPVSGNLRILIVFGEIEYINGGDPTPPNGTTGWPAHSLPTWVNQLTDPT
                     IPSGNATGVITRYFQMASSGSFNVLGDYLLAPDNGGIFKMQTVMNHAITPWDSLDPNY
                     HNANYFLRNEINSKMNQIITGHNLNSIDYFDLWTIPNSTGVLKTTPSTETPRKYDAVM
                     FIWRNSAWNGTGYSTNGSAGNLIGYQSNSFSCFGTHNNIPTQIMIHEFSHGLYGGNNF
                     HCGGGGWNEYFGDYFIPPIGGWSNLGLSGASLLTWNAWDRQRLDWKTPGNTYVVSARD
                     ANGSSEVNGDLDATISSQAGIYTVRDFVTTGDAIRIKLPFLNPSTEYPEYIWIENHNT
                     KSRNGCDWDKFMFEEGNSCINGALYGLYAYLQIDREVRESTTKDNVFGGYAYYLRPLT
                     ANGYWDRLFESTTTQNNCINLAYYNAFTLYKQNPLTGGGDQDVGYSYNRNGNSTLEFD
                     DIMGNYIELLNGTYLKNLVSNGHSRHVFTYAGNNKIGMGTNPSSATMMNMVGYSYYPE
                     MPSYKNVRKISLNGVSIEIIEQNQSTGNIKVRIRFDDVDVNNDVRWCADEIVLNPIAT
                     STGYSLNLTTGKTITLDQTLTDTRMNNPILFDGRNIFASPTVFNIKPNAKVHLEPNAN
                     IILNNGSTMNINSAASCVVEDNGTLEVKSGNTLYIDNCGTLAINGLGKFIARTGSTLC
                     ISPNAVLAFENGLQNLVVESGVTIPAGYVNPASIVQPTINNYTINANTPNWVSQSYLI
                     AGVVTVEPGANFTIQSSTLKFSDINSKLVVKQGAKLTIDNSTLKNSCTGQWQGIEVWG
                     TTNQYQFPINGVYAQGIVELKNGAIVENAANGITNWKPDDWNSIGGIIKAEGVTFKNC
                     RRGVEFMKYRNFHPTSGYEMDNVSYFKDCSFIVDDNYPANASPFYTGVSLWSVRGIKF
                     SGCDFFNNRTIKGSGYGIYSLDAGYKVYAICNSQASPCPEQYLDKTWFRGFEYGINAS
                     NTETSNSVYIQDSKFTENGYGIELSNVNNAVVLKNRFEMTSATNCPNYGYGINLLNCT
                     GYAIEENLFTVNGVPPANNWSGIEITNSGTAYNEIYKNTFQNLTTGNLADLLNATTNT
                     IPPTGLTYLCNTNTNNDYDFYVTNKPESRISYYQRSGTSTAAGNGFSQGANVNFHNGG
                     GTQIRYYYSTGGAPPTYYGVVPVYTANANTCPSHYGGGSGNLAQLNQSQMDALEALYA
                     DNYSAYNNVKSIYETLKDGGSTTNTVEDIETSMPSEMWELRVKLLGDSPHLSQTALKE
                     AALKTDVLPESVLFEILSANPDEMRDESFLAFLESKENPLPSYMIDLLRAIAGNTSYK
                     TVLQEQLNAFNTQKTLAASDIIRSKLNDSIVSQTDIINWFDNRGDLNSRYQMVDAYIQ
                     MGNYAAAQGMLSLIPGLHELSAADSADYQKVCELKNLQINILNAGRNLAMLTNTEKEQ
                     LSVIADANTGKASTQAKGILGFMAGTPYCNCVRYNDELKHSSAKPSTIVNLPSGIKIS
                     VAPNPATTWITFEYSLPEKLTSVTLTITDNTGKKVETISLTGKQGQKVLDIRTYASGA
                     YIYQCNQDKKVSGKFIIK"
BASE COUNT         2028 a         1252 c         1178 g         1777 t
ORIGIN      
        1 cgttagcaac aaggcgtaga aaaaaaacaa acacctaccc acagcaaaag agttgcaaat
       61 tttgactaca cacttacccg ataaaaattt gcaacccatt tgctcttggc tgacacacaa
      121 gaaaaactat taaaaaaata ccattaaatg gtaagggaat ttcaattaat tttagttgcg
      181 taattaacct aggacaatat ttgaacatga aatataaaca cttcgcctta gtaacattaa
      241 tgctaatttt tgcaatactc ataaaagccc agcaaccctg gcgtttccac atcgcttttg
      301 aggatggcag tggccagcga gatacacttt ggatgatctt tgataacaac gctacaattg
      361 gaattgatac actgcttgga gaattaactc ctcaattcga ttcaacagag tttaatgttt
      421 ttatgcaaag ttatgacact atttatacaa aagttcgagc ctatccttat agcttgtacc
      481 catatatgac tactggaagt atatattcaa taaattacac atatccagta attatccgat
      541 gggatactgc cttattccat gccccgtatt tgccgacaag tccaacaatg aatattgcta
      601 ttattgttgg gaattaccct ttttttatgt tgcctattga ccctactgca ccatacttac
      661 acacaataaa tatgttaacc aaagacagct tattttgccc tgaagattta aacggggttc
      721 aattcccttt atttggggat cagtcattgg gatttgcagt tgatcatgat aactacctct
      781 atttggtaga gaaaaaaaca gagcgggttt tgctttatcc taaccctgca aatgaatatt
      841 tgcttgtaag catggaaaat ccagttattt cgttttgtat tactgatatg acgggcaata
      901 ttgtaatttc agaatacttc aatagttacc aaaagatgga agcgtataaa ataaattgtc
      961 aggatttatg tgcaggatta tacttaatag aaattacaaa tttcaaaaat caaaaacact
     1021 atgaaaaatt tatcattact aaataagaaa ttgttatgtg tgattgccat aatggcctta
     1081 acatcaactt tgctgggaca ggtatgccta acaggctcaa ttgaatcaga accttcggat
     1141 aatcctaata cggtttatgc tagtcgattt gggagttatt tgccagtctc tggaaacctc
     1201 agaatcctta ttgtttttgg agaaattgaa tacattaatg gtggtgatcc cacaccacca
     1261 aatgggacaa caggttggcc ggctcactct ttaccaacat gggttaatca acttactgat
     1321 ccaactattc cgagtggaaa tgccacagga gttattactc gctattttca aatggcttca
     1381 tcaggcagtt tcaatgtttt aggagattat ttgttagctc ctgacaatgg gggaattttt
     1441 aaaatgcaaa ctgtaatgaa tcacgcaatt acgccatggg attctttaga ccccaattat
     1501 cataatgcaa attacttctt gcgcaatgag attaattcta aaatgaatca aattataacc
     1561 ggtcacaatt taaacagcat tgattatttc gatttatgga caatacccaa ttcaactggc
     1621 gttcttaaaa caactcctag tacagaaaca ccccgcaaat acgatgcggt catgtttata
     1681 tggaggaact cagcatggaa tggtaccggt tatagtacaa atggttctgc cggaaacctg
     1741 attggatatc aatccaattc attttcctgc tttggcactc acaataacat cccaacacaa
     1801 ataatgatcc atgaattttc acacggctta tatggtggaa ataattttca ctgcggtggc
     1861 ggaggatgga acgaatattt tggtgattat ttcataccac cgataggtgg ctggagcaac
     1921 cttggccttt cgggtgcttc cctgcttaca tggaatgcat gggacaggca acggctcgac
     1981 tggaaaactc ccggtaatac ttatgttgta tcagcacgcg atgccaatgg ttcatctgaa
     2041 gttaatggcg atcttgatgc cacaatttct tcacaagcag gtatatatac ggtgcgcgat
     2101 tttgtaacaa ccggtgatgc catccgtatt aaactaccat tcctcaatcc ctccactgaa
     2161 tatcctgaat acatttggat tgaaaaccat aacactaaga gccgcaatgg atgcgattgg
     2221 gataagttta tgttcgagga aggaaattct tgcataaatg gagcattata tggcttgtat
     2281 gcctacttgc aaattgacag agaagtgagg gaaagtacaa caaaagataa tgtttttggc
     2341 ggatatgcct attatctcag gccattaacg gccaatggat attgggacag gctattcgaa
     2401 agcaccacca cacaaaacaa ttgtattaat ctggcgtatt ataatgcctt tacactgtac
     2461 aagcaaaacc cgctaacagg tggaggcgat caagatgtag gttacagtta taacagaaac
     2521 ggtaattcaa cacttgaatt tgatgatatc atgggaaatt acattgagct gctcaacggt
     2581 acatacctaa agaatttagt atccaatggt cattcacgac atgtatttac atatgccgga
     2641 aataataaaa tcggcatggg aaccaatcct tcatcggcaa ccatgatgaa tatggtcggg
     2701 tatagttatt accccgaaat gccatcatac aagaatgtac gcaaaatatc gctcaatggg
     2761 gtgtctattg aaataattga acaaaaccaa agtacaggaa acattaaagt ccgtatcagg
     2821 ttcgacgacg tagatgttaa caatgatgta cgctggtgtg ccgatgaaat tgttttgaac
     2881 ccgatcgcca caagtaccgg ttattcactt aacctgacaa cagggaaaac cattacgctg
     2941 gatcaaacgc ttactgatac ccgcatgaat aatcccatac tgtttgatgg tcgcaatatt
     3001 tttgccagcc caacggtttt caatatcaag ccaaatgcaa aagttcatct cgaacccaat
     3061 gctaacatca tacttaataa cggtagtaca atgaatatca attctgctgc ttcttgtgtt
     3121 gtagaagaca atggtacgct ggaagtaaaa agcggaaata cattgtatat tgataattgt
     3181 ggtactttgg caataaacgg tttgggaaag ttcattgccc gtacaggttc aactttatgc
     3241 atctctccca atgcagtgct tgcttttgaa aatggcttac aaaatttagt tgttgaaagt
     3301 ggtgttacta ttccggctgg ttatgttaat cctgcttcaa ttgtgcaacc cacaatcaat
     3361 aattatacta tcaacgccaa tacaccgaat tgggtttcac agagctacct aattgccggt
     3421 gttgttactg ttgaacctgg tgctaatttt accattcaat catcaacctt aaaatttagt
     3481 gatataaaca gtaaacttgt tgtgaaacaa ggtgcaaaat taactattga caactctact
     3541 ttgaagaatt cctgcaccgg ccaatggcaa ggcatcgagg tatggggcac aaccaaccag
     3601 taccagttcc cgataaatgg agtttatgct cagggtattg ttgagcttaa aaatggtgct
     3661 attgtagaaa atgcagccaa tggcatcacc aactggaaac ccgatgactg gaattcgata
     3721 ggaggaataa taaaggccga gggtgtaacc ttcaagaatt gcaggcgtgg tgttgagttt
     3781 atgaaatacc gcaattttca tcctacatca ggttatgaaa tggacaatgt aagctacttt
     3841 aaagactgtt cattcattgt tgatgataat tatcctgcca atgcctctcc tttctataca
     3901 ggagtttcat tatggtcagt aagagggata aaattctcgg gctgcgattt cttcaacaac
     3961 agaacgataa aaggtagcgg ttatggtatt tattcattgg atgccgggta taaagtttat
     4021 gctatttgca attcgcaagc atctccttgt ccggaacagt atttggataa aacctggttc
     4081 agaggatttg aatatggcat caacgcatcc aataccgaaa cctccaactc ggtgtatata
     4141 caggattcaa aatttaccga aaacggctac ggtattgaat taagtaatgt aaacaatgca
     4201 gtggtgctta aaaatcgttt tgaaatgaca tcggctacca attgccctaa ctatggctat
     4261 ggcataaacc ttttaaactg taccggatat gctattgagg agaacctctt tacagtaaat
     4321 ggtgtaccgc cagccaataa ttggtcgggt atcgaaatca ccaattcagg cacagcatac
     4381 aatgaaatct acaaaaacac attccaaaat cttactacag gaaatttggc tgacctttta
     4441 aatgcgacga caaatactat tcctcccacc ggtttgacat atttgtgtaa caccaacacc
     4501 aataatgatt acgattttta tgttacaaac aaacctgaat cgcgcatttc atactaccag
     4561 cgcagtggta cttcaacagc agcaggaaat ggtttttcgc agggtgcaaa tgttaacttt
     4621 cataatggcg gtggcacaca aatcaggtat tattattcaa caggcggtgc tcccccaacc
     4681 tattatggag ttgtgccggt ttacacagcc aatgccaata catgtccatc gcattatgga
     4741 ggtggttcag gcaatttggc tcaactcaac caatcacaaa tggatgcatt agaggctcta
     4801 tatgccgata attattcagc ctacaataat gtaaaatcca tttatgaaac cttaaaagat
     4861 ggaggaagta caacaaatac agtagaagat attgaaacca gtatgccatc cgaaatgtgg
     4921 gaattaaggg taaaactatt aggcgactca cctcaccttt cgcaaacagc acttaaagaa
     4981 gcagccttaa aaacggatgt tttacctgaa tctgttcttt ttgaaattct ttcagccaac
     5041 cctgacgaaa tgcgtgatga gtcatttctc gccttcctcg aatcgaagga aaatccgctt
     5101 ccttcataca tgattgattt gctcagggca attgctggca acacatcata caaaacggtt
     5161 ttacaggaac aactcaatgc tttcaataca cagaaaactc tggctgcttc tgatattatt
     5221 cgcagcaaac ttaatgatag tattgtaagc caaacggata tcatcaactg gtttgataat
     5281 cgtggcgatt tgaatagccg ataccaaatg gttgacgctt acattcaaat gggtaattat
     5341 gctgcagcgc agggtatgtt gagcttaatc cccgggttgc atgaattatc tgcagccgat
     5401 tcagcagatt accagaaagt atgcgaattg aaaaacctgc agattaacat tctaaatgca
     5461 ggtagaaacc tcgctatgct aacaaatact gaaaaggaac aattatcggt tattgctgat
     5521 gccaacaccg gaaaagcttc cactcaggca aaaggtatac tcggatttat ggctggaaca
     5581 ccatattgca actgtgtgcg atataacgat gaactcaagc acagctcggc aaaaccatca
     5641 actattgtta atttaccatc cgggataaag attagtgtag ctccaaatcc ggcaacaaca
     5701 tggattactt ttgaatactc cttaccggaa aaattaacct ctgtaacact cacaattact
     5761 gacaatacag gcaaaaaggt tgaaacgata tcattaaccg gtaaacaagg tcaaaaagtg
     5821 ctggatataa gaacttatgc ttccggtgca tacatttatc aatgcaacca ggacaaaaag
     5881 gtttcgggta aattcataat caagtaataa caaccagaat catttttatg aactcacggg
     5941 gcaacatttc tgcctcgtgg gttaattttt tataaaatat gacagaggta atgcacttaa
     6001 tccacccttc gcagacaagc acattgtcaa gtcacacctg cctgcgccaa tgcctaaact
     6061 taccaaagag cttgtctgcc ccaacgcaca gacagaagaa cgcccagttg ctaacaggcg
     6121 gtatatttta ttgcctgatt tgtactaaat tcactatttt tacaactaat ataacttgta
     6181 ctgccttgac agtgaatact tctaagtccg gcaacaaaac ataccgccaa ccgtt
//
LOCUS       DAOPYB010000177         6215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_15556_length_6215_cov_9.887662, whole genome shotgun sequence.
ACCESSION   DAOPYB010000177 DAOPYB010000000
VERSION     DAOPYB010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15556_length_6215_cov_9.887662"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /gene="tsaA"
                     /locus_tag="PKH58_11065"
     CDS             complement(<1..347)
                     /gene="tsaA"
                     /locus_tag="PKH58_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNX89616.1"
                     /translation="MAALKIIARIHTDFPTKFGVPRQSGLIDTLKSMIVFEPEYRNPD
                     ALRGLEDFSHIWLIWGFSEAERDGWSPTVRPPRLGGNRRMGVFATRSPFRPNSIGLSS
                     VKLDAISKHPEFGT"
     gene            complement(821..1450)
                     /locus_tag="PKH58_11070"
     CDS             complement(821..1450)
                     /locus_tag="PKH58_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNX89617.1"
                     /translation="MKKLFLLIIAVCMYLLPASAQQTVAVMLDIQKNSVLSIQGTTNI
                     APFRLYQNSDKFIPKNLLITASKVRDKLFIHENKITVPVNQFSSNNKMALRDFFKLMK
                     SDTYPELQIKLDYIELTQSSSLPQGNAIVDVTITGITKKYSFPIQTRKEGNNYLFNIR
                     KDINIHDFRLTPPKQILGMIKVNEWINIDLSMTCNIQAIKQVELYSDNL"
     gene            1669..2739
                     /locus_tag="PKH58_11075"
     CDS             1669..2739
                     /locus_tag="PKH58_11075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX89618.1"
                     /translation="MEKISEDKVVIRDADKSDNEGLLSLAASSGMRGKFSFRVERKPD
                     FFRLLQRKGNYLVKVAVMEGRIVGSVSVVEMDIFIGCKSSKAYYACDFRVHPDYRGSG
                     LGVRLVQEIYHQSVSQGAELFFGVMLLGNREAASYLSGKLRAVPPIVAGSVFRVFQII
                     PSATEIKSSKYRIETVDDFRNELFSEEMKQYSFAHDYPNDIYKDTRIWCAAENGVVVA
                     AIALAASEEFKQEVLVDVPGYLKPLMKLAEQVKWINSRMNLPRLNGNIRVLYIRSFYG
                     RDEHQEALRILIAHARNQAYKQQYHFLTVGIDERSKYLRFFSSFPKFTLKSRLYFCNS
                     GIAPDVFDALRQGVSFVDYSLV"
     gene            complement(2751..3833)
                     /locus_tag="PKH58_11080"
     CDS             complement(2751..3833)
                     /locus_tag="PKH58_11080"
                     /inference="COORDINATES: protein motif:HMM:NF023327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HNX89619.1"
                     /translation="MAFLVDFGISQEDYRTEEEILQIGEEDHILSLASAGEVPLCILS
                     TRDNIRLTAVDISEKQIYLTKLKFTSALQLDFPLNGRFLGYGKLDAATREVIYRKQIR
                     DHLTAEESAFWDSNLKYLKMGVINSGRFEHYINQLRFLLYMIIGKKNIIDLNNCHTTN
                     EQTEIFDRKIASRKSLQWLFKIAFHPAIYRKRGLQDQALIHADGTTGERFYAKFRNFC
                     TATPASDNYFLQYFLTGACKTETAFPPYLKPENKQRLLENLTRFELIHSSFQDELSRK
                     EMGYYNKIHISNLGDWTGETEFEKLIGLLKEQLNPGTRICYRYLQKNYFRDSYGDLFD
                     KDEQAAISAATTDRFPFYNLVALSRK"
     gene            complement(3949..4446)
                     /locus_tag="PKH58_11085"
     CDS             complement(3949..4446)
                     /locus_tag="PKH58_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNX89620.1"
                     /translation="MKILTIVRHAKAEQPEPPQSDFDRKLTPRGKRNAAEMASYLHTR
                     GFKPGLIISSPAKRALQTASIFSDEFSFDKQKIVKENFLYSRYDVPEIREMLNLDAKS
                     TDSVFLFGHNPALSWLAATLAPGFGQGLPTAGVVIIEFNTNDWNEIDATNGKVVLYKY
                     PANLI"
     gene            complement(4637..4707)
                     /locus_tag="PKH58_11090"
     tRNA            complement(4637..4707)
                     /locus_tag="PKH58_11090"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4673..4675),aa:Gln,seq:ctg)
     gene            4864..5373
                     /locus_tag="PKH58_11095"
     CDS             4864..5373
                     /locus_tag="PKH58_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNX89621.1"
                     /translation="MKKLDENFFRRDAVVVACELLGKTLVRFHDDGTTCRYRITATEA
                     YLGGDDLACHASKGRTPRTEVMFHGGGKIYVYLIYGMYWMLNIVTGVEDHPQAVLICG
                     LNEVKGSGRVGKLLKIDKSFYGEDLTGSTRIWLEDSETYTNYIYKPRVGVDYAGEWKD
                     KLWRFETLD"
     gene            complement(5527..>6215)
                     /locus_tag="PKH58_11100"
     CDS             complement(5527..>6215)
                     /locus_tag="PKH58_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNX89622.1"
                     /translation="TRGAFEYQGQKCSAASRAYIPSNIWKDVKKYLTEDLERIKTGDV
                     MDFSNFVNAVIDEESFDKLAGYIDNVRNSSDAEIVAGGTYDKSTGYFIHPTVILAHKP
                     DYITMREELFGPVLTVFVYEPERMDETLDILDRSTAYALTGAIFCAERSNIEYLTRRL
                     THTAGNFYINDKPTGAVVDQQPFGGGRASGTNDKAGSIFNLLRWVSPQCIKETFVPAT
                     DYMYPNFRKE"
BASE COUNT         1715 a         1204 c         1406 g         1890 t
ORIGIN      
        1 gttccgaatt cgggatgttt gctgatggca tcgagtttta cagaagaaag tccgatggaa
       61 ttcgggcgga aaggggagcg tgtggcgaaa actcccatgc gccggttacc gccaagtcgt
      121 ggaggacgta ccgtgggcga ccatccgtca cgttcggctt ccgagaaacc ccagataagc
      181 cagatatgag agaaatcctc caatccgcgc agcgcgtcgg gattgcggta ttccggctcg
      241 aatacaatca tggacttcag tgtgtcaatc agtccgctct gacgcggaac gccgaacttg
      301 gtagggaagt cggtgtggat gcgggcaatg attttgagtg ctgccatata attgggttcg
      361 gtgaatgatt ttattttatt accggacaaa tttacaaaaa tatgatggtg catttcattt
      421 tgacagtata aaacatcaaa ggataatccg taaagtaaac ctctaggggt ttcaccccta
      481 aaacccgact tacttttttg tcttgccaca aaaaagtaag caaaaaaagt caagactgtg
      541 cccgcttcac tcgaaaaatt tgcgctcgaa tggctaaatc gtccaaactc gttgaagttc
      601 gcccaaaatc tatgtatttt gaaataattc tatgcgaact tcaacttcaa acaaggacga
      661 tttttaacgc cattctcact gatttttcgg ctcaccggac gaggtcagtg gttggcgaac
      721 cgaaatacaa tgatcaagag attatgtgga taatcattaa atattaaaag tgctgttttg
      781 aggttctcaa aacagcactt tcaaaataaa aaataactat ttacaaattg tcactatata
      841 attcgacctg tttaatggct tgaatgttac atgtcatgct caaatcaata tttatccatt
      901 cattcacttt gatcataccc agtatttgtt tcggaggtgt taacctgaaa tcatgaatgt
      961 tgatatcttt cctgatatta aaaaggtaat tatttccttc tttccgggtt tggatgggga
     1021 atgagtattt ttttgtaatc cccgtaatcg taacatcaac aatggcatta ccctgtggca
     1081 gcgaactaga ctgcgtcagt tcaatgtaat cgagcttgat ctgcaattcg ggataggtgt
     1141 ccgatttcat cagtttgaaa aaatccctca atgccatttt attgttcgaa gaaaactgat
     1201 tgacaggaac cgtgatttta ttctcgtgaa taaacagctt gtccctgacc tttgacgcgg
     1261 taatcagcaa attcttcggt ataaatttat cgctgttctg gtaaagcctg aagggggcga
     1321 tgttggttgt tccctgaatg gaaagtacac tgtttttctg aatatcaagc atgacggcca
     1381 ccgtttgttg agcggatgcc ggtaataaat acatacaaac tgcgataatc agaaggaaca
     1441 attttttcat tgagtcttat gctgtaaaaa attccaacca tactattagt gtataacggt
     1501 atggttggat aaatgtttta ttaaattccg aactattatt cagcggattt cgttactttt
     1561 aataatttgc ttttataaac taatgcagat attcgctgct gattattccc tgttctcaat
     1621 aacagaaaaa taagagactt tagcgcttaa acatgttaat cccgataaat ggaaaaaatt
     1681 tctgaagata aagttgttat tcgtgatgct gataaatccg ataatgaagg actgctcagt
     1741 ctggctgctt catcaggcat gcgcgggaag ttttcttttc gtgttgaaag gaaacctgat
     1801 tttttcaggt tgttgcaacg taagggaaac tatctggtaa aggttgctgt aatggaggga
     1861 aggattgttg gctccgtttc ggttgtagag atggatattt ttattggatg caaatccagc
     1921 aaagcttatt atgcatgcga tttcagagtt catcccgatt accgtggttc gggactgggt
     1981 gtgagacttg ttcaggaaat ctaccaccaa tcggtgtccc agggagcgga attgtttttc
     2041 ggagtgatgc tattgggcaa cagagaagct gcatcctatt tgtcggggaa actcagagct
     2101 gtaccaccca tagtagcggg aagtgttttt cgtgtatttc agattatccc ttctgctacg
     2161 gaaataaaaa gcagtaaata caggattgaa actgttgatg atttccggaa tgagttattt
     2221 tcggaggaaa tgaagcagta cagttttgca cacgattatc cgaatgatat ttataaggac
     2281 acccggatat ggtgtgcagc cgaaaacggg gttgttgttg ccgcgatagc gcttgccgct
     2341 tcggaggaat tcaaacagga agtgctggtg gatgtgccgg gatatctcaa acccttgatg
     2401 aagttggcag aacaggtgaa atggataaac tcacggatga atctgccccg gctgaacggg
     2461 aacatccgtg tcctttacat ccgctcgttt tacggccgcg atgaacatca ggaagccctg
     2521 aggattttga tagctcatgc tcgtaaccag gcttataagc aacagtatca ttttcttacg
     2581 gtgggtatag atgaacgaag taaatatctc cggttcttca gttcttttcc gaagttcaca
     2641 ctgaaatcaa gactttattt ttgcaacagc ggcatagcac cggatgtttt tgacgcgttg
     2701 cgtcagggtg tttcgtttgt ggattattct ctggtctgaa agcctgctga ttattttcgg
     2761 ctcaatgcca ccagattata gaacggaaat ctgtccgtag ttgctgctga tattgcagcc
     2821 tgctcatctt tatcgaacaa atcaccataa ctatccctga aataattttt ctgtaaatag
     2881 cggtaacaaa ttctggttcc gggatttaac tgttccttca gcagtccgat taatttttca
     2941 aattcggttt caccggtcca gtcaccgaga tttgaaatgt gaattttgtt ataataaccc
     3001 atttctttcc gtgacagttc gtcctggaag gatgaatgaa tcagttcgaa acgtgtgaga
     3061 ttttcaagca gtctttgctt gttttccggt ttcaggtagg gtggaaatgc cgtttccgtt
     3121 ttgcatgcac ccgtgagaaa gtactggaga aaataattat ccgatgcggg agtggcggta
     3181 cagaagttcc ggaatttagc gtaaaaccgt tcgccggttg tgccgtcggc atgtatcagc
     3241 gcctggtcct gcagtcctct ttttcggtat attgccggat gaaaagcgat tttgaagagc
     3301 cattggaggc ttttacggct ggctatcttc cggtcaaaaa tttcagtctg ttcattggtt
     3361 gtgtgacagt tattcagatc gatgatgttt ttctttccaa taatcatgta cagcaagaat
     3421 cgaagctgat tgatataatg ttcaaatctt cccgaattaa taactcccat cttcaggtat
     3481 ttcaggttgc tgtcccaaaa ggcagattct tcggctgtga gatgatcccg gatttgtttt
     3541 cggtaaatta cttccctggt tgctgcatca agttttccgt acccgagaaa cctgccgttc
     3601 agtggaaaat caagctgcaa agctgatgta aatttaagtt ttgtaaggta gatctgtttt
     3661 tcggaaatat caacagccgt cagccgtatg ttgtcgcgtg tgctgaggat acaaagcgga
     3721 acttcaccgg cactcgccag cgacaggata tgatcttctt ctccaatttg gagaatttct
     3781 tcttctgtgc ggtaatcttc ctgtgaaatg ccaaaatcaa ctaaaaacgc catacttcat
     3841 attgcttaaa tcatcaaatt gtaagatgca gaaatttgaa tgcagcaaaa tccaatagaa
     3901 aaatgatgtt cgaacttaaa tcgagaagat actttaagtg atatgttgtt aaatcaaatt
     3961 ggccggatat ttatacagca caacttttcc gttggtggcg tctatttcgt tccagtcgtt
     4021 ggtgttgaat tcaataatga caactccagc tgtaggcagt ccctgaccaa aacccggagc
     4081 gagggtggcc gcaagccatg agagtgcagg attgtgacca aaaaggaaaa cagaatctgt
     4141 tgattttgca tcgaggttga gcatctccct gatttccgga acatcgtagc ggctatacag
     4201 gaaattttct ttcacgatct tctgcttgtc gaaagaaaat tcatcggaga aaatagaagc
     4261 tgtttgtagc gcacgtttag ctggacttga aataatcaaa ccgggcttaa atcccctggt
     4321 gtgaaggtac gaagccatct cggctgcatt tctttttccc cgtggagtaa gtttgcgatc
     4381 aaagtcggat tgcgggggct ccggttgttc ggctttggcg tgacggacaa tggtaagtat
     4441 tttcatgtga acagatttct tttatcatta aagtaaagtt ttaaagtcgt tgaactaaaa
     4501 aaacaaaagt aaatattctt ttatagaaat aacatttcag caataatgtt tgttgcatta
     4561 tcctgttctg tatgtatatt gtttttattg gtaaaatgac aaacggcaat acagataatt
     4621 gtattgccgt ttattgttgt cccaccaaga ttcgaacttg gacaaacaga ttcagagact
     4681 ggtatgctac cattacacca tgggacagtt tcggactgca aaattacata aatatttcaa
     4741 attgcaaaat gaaccgtaat aagattttta ttctgaaata tattttttaa gtaaaaccca
     4801 tttcaaaatc gttgtttcag acgaatgttt gtatttttgc cgttgaatta atgcgtatta
     4861 cgaatgaaaa aactggatga aaattttttt cgccgcgatg cggtggtggt tgcctgtgaa
     4921 ctgttgggca aaactctagt gcggttccac gacgatggta caacttgccg ttaccggatt
     4981 acagctaccg aagcgtatct gggtggggat gacttagctt gccatgccag caagggacgc
     5041 acaccgcgca cagaggtaat gtttcacgga ggtggtaaaa tatatgtgta tctgatttac
     5101 ggcatgtact ggatgctgaa tatcgttacc ggcgtagagg atcatccgca ggcagtgctt
     5161 atttgcggat tgaacgaagt taaaggatcg ggtagggtag ggaaactgtt gaaaattgac
     5221 aaaagtttct acggcgaaga tttaaccggt tcaacacgca tttggctcga ggacagcgaa
     5281 acatacacaa actatatcta caaaccccgg gtgggagtgg actatgccgg tgaatggaaa
     5341 gataagttgt ggcggtttga aacactggat tagttcacgt ttgacgtttg atgtttgatg
     5401 ttcaatgttt gtgaatcagg tcaattattc attacaaagt tcaatagtta aacttttcaa
     5461 ccttatatag gtttggacta acctgaacca atgatcgttt accttcaaac gtcaaacgtt
     5521 ttatcttcat tctttcctaa aattcggata catataatca gttgccggca caaacgtttc
     5581 cttgatgcat tgcggtgata cccatcgcag cagattgaat atcgaacctg ctttgtcgtt
     5641 ggtaccagat gctctgccac caccaaaagg ttgctgatca actaccgcac cggtaggttt
     5701 gtcgttgatg tagaagtttc ctgctgtatg ggtgagtcgt ctggtcagat attcaatgtt
     5761 acttctttcg gcacagaaaa tcgctcctgt cagtgcatac gcggttgatc ggtcgagtat
     5821 gtcgagagtt tcatccatcc tctcgggttc gtatacaaac acggtgagta ccggtccgaa
     5881 cagttcttcc cgcatggtga tataatcggg tttgtgtgcc agtattactg ttggatggat
     5941 aaagtaacct gtggatttgt cgtatgttcc gcctgccacg atttctgcat ccgacgagtt
     6001 gcgcacgttg tcaatgtagc cagcaagctt atcaaaggac tcttcatcaa taacggcatt
     6061 tacaaagttg gagaaatcca tcacgtcgcc tgttttaatt ctctctaaat cttcggtaag
     6121 atacttcttt acgtccttcc agatgttcga aggaatataa gcccgcgaag ctgccgaaca
     6181 tttctgtccc tgatattcga atgcaccccg ggtga
//
LOCUS       DAOPYB010000178         6160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_15750_length_6160_cov_8.932842, whole genome shotgun sequence.
ACCESSION   DAOPYB010000178 DAOPYB010000000
VERSION     DAOPYB010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15750_length_6160_cov_8.932842"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PKH58_11105"
     CDS             complement(<1..1010)
                     /locus_tag="PKH58_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX89623.1"
                     /translation="MNKQKQSTSRTFSPWTWIPTLYFAEGIPYVVVMTVAVIMYKRLG
                     ISNTDIALYTGLLYLPWVIKPFWSPFVDLIRTKRWWIITMQLILGAGLAGVAFLIPAP
                     FFFQATLAVMWLIAFSSATHDIAADGFYMLALNSSQQSFFVGIRSTFYRLAMITGQGI
                     LIILAGFLETSTGLEPLELNVNSTKKIVQTDIWKNAATAELSNSSEAVFTAFPSMLEI
                     QTATISPDSLQKIKDFAAGENLKNGFVQTEKKIEKKKNWWQRHVGAQWTNLVSEPLGN
                     FISRNFGKKTGEQVSASGKVGNAAIVAVKLNKKPEAGKETILNTRLSSGDKSIALIGG
                     ERL"
     gene            1396..2748
                     /locus_tag="PKH58_11110"
     CDS             1396..2748
                     /locus_tag="PKH58_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX89624.1"
                     /translation="MSGSTLSAAKVVRPIKTAVPARPKGQNDVVNLVTPKLDVVRVGF
                     IGLGMRGPGAVERWTHIPGVKIVALCDLLPEHVTNCQKTLQKADLPAAAEYSGSTDAW
                     KKLCERDDIDLVYIATDWKHHAEMALYAMEHGKHTAIEVPAAMNMKEIWALINMSEKT
                     RLHCMMLENCVYDFFELTTLNMAQKGVFGEVLHVEGSYIHNLEEFWPYYWNNWRMDYN
                     QTHRGDIYPTHGMGPACQLLDIHRGDKMNYLVAMDTKSVNGPAYIKEKTGKEVTDFKN
                     GDHTMTFIRTEKGKTIQIQHDVVTPRPYSRMYQLTGTDGFAEKYPVEGYCLRPSQVSS
                     TDVPNHEDLNAHGFVPENIKSALMAKYKHPIHQELEEIAKKVGGHGGMDFIMDYRLVY
                     CLRNGLPLDMDVYDLAEWCCMTELTAISLDNNSAPVEIPDFTRGAWNKVQGYRHAFAK
                     "
     gene            complement(3019..3738)
                     /locus_tag="PKH58_11115"
     CDS             complement(3019..3738)
                     /locus_tag="PKH58_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HNX89625.1"
                     /translation="MEIFWETIALYNYSTWVYQLAFIVMGLVFTVILIHNPREWVKLA
                     MKIYLIVLCLWIAVVYYHIYCSERKFNNVLTVFWSIMAGVWIWDAIKGYTTFNRSYKH
                     DYLAYLLLLMPFLYPLISVLRGLTFPEITTPIMPCSVAVFSIGILLLFSEKINMIIVL
                     FLCHWSILAISKTYFFNIPEDFLLASASVPALYLFFKEYFVRDLHKDTKPKAKYISLL
                     LVFMSVIIGVLLTVTFFAGLK"
     gene            complement(3745..4245)
                     /locus_tag="PKH58_11120"
     CDS             complement(3745..4245)
                     /locus_tag="PKH58_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HNX89626.1"
                     /translation="MILVDILSDGFFAAIASIGFGAISDPPLKAFKFIAILAAIGHAC
                     RFCLMTYFSFDIASASFISALIIGFGSLWLGGKIYCPMTVIYIPALLPMIPGKFAYKM
                     VFSQIMFIQNMDEPALRTKYMDMFFSNSMITFTVIFLLAIGATLPMFIFHTKAFSLTR
                     HITKKD"
     gene            complement(4242..5009)
                     /locus_tag="PKH58_11125"
     CDS             complement(4242..5009)
                     /locus_tag="PKH58_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HNX89627.1"
                     /translation="MKTNETIVSQTKFIAEYATHLMGCGVHTSRVVRNTKRIGESLDF
                     DVQISVFQKSLILTMLDIETQKTYSEVNEIPVIPISFEQNSELSALSWEAVDNRLTFE
                     ETKKKYQKIVESPQMDTLFVLLLVGFANASFCRLFGGDWISMAIVFSATIAGFFVKLQ
                     MQKNKFNHYITFIISAFVASLFSATSLIVDITSEIALATSVLFLIPGVPLINGVIDIV
                     EGHTLTGISRLTNAFLLIVCVAIGLSFTLFLVKDKLI"
     gene            complement(5096..>6160)
                     /locus_tag="PKH58_11130"
     CDS             complement(5096..>6160)
                     /locus_tag="PKH58_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89628.1"
                     /translation="TFGINDYTSHELKQGTLSDQFKSFSARKNLGQKLCSTPVLFNSR
                     NYLSAWSNTVRNHNFIFSDFKQIETNIQLNKVKQDEQQGLYLGRYIRFTSGLEDSTLM
                     KYNEVVGFDGKKFIISSEAEEFVTKTVQLCKENNVKLVFLTLPMYYRHFKDMDAYVAK
                     VAKVLEPQKQLWDNQQSPYDYKAFTPECFENTVKENQHMTYYGSLVSTYKLASFIQSN
                     LAGVLPDRSKDIEWKKLFYGYDGYFYNHPPENDGISQVLISNQTMGGVPVKEIELVPS
                     QQGFKQLLVKVNKNSGIPVSAKTIRVMLDVNVNGQNQTTVVDAVSSLAYDPSGHYLFA
                     SQPLDYGVNAVRVRNISLIN"
BASE COUNT         1934 a         1292 c         1214 g         1720 t
ORIGIN      
        1 agacgttcac cgccaatcag cgcaatactc ttgtcgcctg acgataagcg ggtgttgagt
       61 attgtttctt tgcccgcttc gggcttttta tttagtttta cagcaacaat tgccgcattc
      121 cccactttac ccgaagcaga aacctgttct ccggttttct tgccaaaatt ccggcttata
      181 aaatttccaa gcggttcaga cactaaattc gtccattgag ccccgacatg gcgttgccac
      241 cagttctttt tcttttcaat tttcttttct gtctgaacaa aaccgttttt cagattttca
      301 ccggcagcga aatctttgat cttttgcaga gaatcgggac tgatagttgc agtctggatt
      361 tcgagcatgg acgggaacgc cgtgaaaaca gcctcggatg aatttgataa ttctgctgtt
      421 gccgcgtttt tccagatatc tgtctgtact attttttttg tcgaatttac attcagttcc
      481 agaggttcaa gtccggtact tgtttccaga aaaccggcaa gaataatcag aataccctgt
      541 ccggtaatca tggccagtcg gtagaatgtg cttcggatac cgacaaagaa tgactgctgc
      601 gaactattga gcgccagcat ataaaatccg tcggcagcaa tatcatgcgt agctgaactg
      661 aaagcaatga gccacatgac agccagagta gcctgaaaaa agaaaggagc aggtatcagg
      721 aatgccacac cggccagacc ggcacccaat atcaactgca tcgtaataat ccaccagcgc
      781 tttgtgcgaa tcagatccac aaacgggctc cagaaaggct taatcaccca gggcaaatag
      841 agcaaaccgg tataaagcgc aatatcagta ttggagatac ctaaacgttt gtacatgatg
      901 accgcaacag tcattactac aacataggga ataccttcgg cgaaatagag agtaggtatc
      961 catgtccagg gagaaaaagt tcttgaggtg gattgtttct gtttattcat tggtattggg
     1021 actaaagatt atattttatc acaaaattaa ttaattagcc cgatataaca gtataaaatt
     1081 ttcatttttc ataaaaaaca gttctgtcac ttcacgatat cggaatcaca aatagcaggc
     1141 tataatcatt ctcagaacaa atgattttgg aattattcaa caaatgactg attaaatccc
     1201 acatttcgaa tttagatgtt tttgaacttt attgtgatat ttttcataga gcattgaaat
     1261 taacaacacg atattttcag ataaatggtt acttttgtga ttctgtttaa ccaacaaaaa
     1321 aatctataaa acatcaaatc tatgaaaaga ctttttagct ttctgatgat tgcactgttg
     1381 ctgcttggtt ccgggatgtc cggttcaact ctcagtgcag ccaaagtagt aagacccatc
     1441 aaaacggcag ttcccgcccg tccgaaagga caaaacgacg ttgtaaactt agtaacccca
     1501 aaactggacg tagtccgcgt tggatttatc ggtcttggga tgcgtggtcc cggtgctgtg
     1561 gaacgctgga cacacatacc cggtgtaaaa attgttgccc tctgcgattt gcttcctgaa
     1621 catgtgacaa actgtcagaa aacactacaa aaagcagact tacctgcagc tgcagaatac
     1681 tcaggcagca ctgacgcgtg gaaaaagctt tgcgaacgcg acgatatcga ccttgtgtac
     1741 atcgctaccg attggaaaca ccatgctgag atggccctct atgctatgga acacggaaaa
     1801 cataccgcta tcgaagtgcc tgccgccatg aacatgaaag aaatctgggc gctcatcaat
     1861 atgtcggaaa aaacacgtct gcattgcatg atgctcgaaa actgcgttta cgactttttt
     1921 gaactgacta ccctgaatat ggctcagaaa ggcgtattcg gcgaagtgct gcacgtagag
     1981 ggatcttata tccacaatct ggaagaattc tggccttatt actggaacaa ctggcgtatg
     2041 gattacaacc agactcaccg cggtgatatt tatcctaccc acggtatggg tccggcctgc
     2101 caattgcttg acattcaccg tggcgacaaa atgaactatc tcgtagctat ggataccaaa
     2161 tctgtaaatg gacccgccta tatcaaagaa aaaaccggca aggaagttac cgatttcaaa
     2221 aacggtgacc acaccatgac tttcatccgc accgaaaaag gcaaaacaat ccagatacaa
     2281 catgatgtag taaccccacg tccttacagc cgtatgtatc agctgaccgg caccgatggt
     2341 tttgctgaaa aatatccggt tgaaggatac tgtctgcgtc cttcacaggt ttcttcgacg
     2401 gatgttccca atcacgagga cctgaacgcg catggctttg ttcccgaaaa cataaaatca
     2461 gccctgatgg caaaatacaa acaccctatt catcaggaac tggaagaaat tgccaagaaa
     2521 gtgggcggtc acggtggtat ggacttcatc atggactatc gcttggtgta ctgtctgcgc
     2581 aacggacttc cgctggacat ggacgtatat gacctggctg aatggtgctg catgaccgaa
     2641 cttacagcta tttctctgga caacaacagt gctccggtgg aaattcccga cttcacacgc
     2701 ggtgcctgga acaaggttca gggttacaga catgcttttg caaaataaaa atctgaaaaa
     2761 caattagcta aagaaaagga tgtacaatag gtattttgtg actttttgta aatcagctaa
     2821 gtagctgtta aacaaacttt ctctcctacc cctcgtttgg aaacgagggg ttttttgttc
     2881 tctatgattg caacataaaa aatcaaatat cttatctatt tcaataaccg cactcaaacc
     2941 tgcataatcc gttttcttta cagagttatt tttgatgtat ctatcaaatc aaacagatta
     3001 cacaaactga atttatcatt attttaatcc agcaaagaag gtgactgtta atagaacgcc
     3061 aattatgacg ctcataaaaa caagtaataa gcttatatat tttgctttag gcttagtatc
     3121 tttgtgtaaa tcgcgtacaa aatactcctt aaagaacaga tatagtgcag gaacggaggc
     3181 gcttgccaaa agaaaatctt ccggaatatt gaaaaaatag gttttagaaa tggctaatat
     3241 agaccagtga caaagaaaaa gaacaattat catgtttatt ttttcggaga ataacaacaa
     3301 aatccctatg gaaaaaactg caaccgaaca gggcataatt ggtgtagtaa tttcaggaaa
     3361 cgtcaatcca cgtaaaacag atattagcgg gtataaaaaa ggcatcagta acaacaaata
     3421 ggcaagataa tcatgcttgt agctgcggtt aaaggtggta taacctttaa tggcatccca
     3481 aatccataca cctgccatta ttgaccagaa tacggttaac acattgttga attttcgttc
     3541 ggaacaataa atatgataat aaacaactgc aatccataag cagagtacaa tcaggtaaat
     3601 cttcattgct aattttaccc attccctcgg attgtggata agaataacgg taaaaaccaa
     3661 acccatgacg ataaatgcaa gttggtaaac ccatgtagaa taattataaa gagctatcgt
     3721 ttcccagaat atttccataa cttattagtc ctttttggtt atatgacgcg tcaatgagaa
     3781 cgctttggta tgaaatataa acatgggcaa agtggctccg atagctaata aaaatattac
     3841 tgtaaatgta atcatgctat tagagaaaaa catgtccata tattttgtcc gcagtgcggg
     3901 ttcatccata ttttgtataa acataatttg cgaaaagacc attttgtacg cgaattttcc
     3961 gggtatcatg ggcaaaagag ccggaatgta aattacagtc atagggcaat atatctttcc
     4021 accaagccaa agacttccaa agccgatgat caatgcactg ataaatgatg ccgatgcaat
     4081 atcgaaactg aaataggtca tcagacagaa acggcacgca tgaccgatgg ctgcaagtat
     4141 cgcaataaat ttgaaagcct tcaaaggcgg atcggaaata gcgccaaagc ctatagaagc
     4201 aatggcagca aaaaatccgt cggaaagaat atctacaagt atcatatcaa tttgtcttta
     4261 acaaggaata atgtaaacga tagtccgatg gcaacacaca caataagcaa gaaagcattt
     4321 gtcaaacggc taatacccgt caatgtatgt ccttcaacta tatcaataac gccattgata
     4381 agtggaacac cgggtatcag gaacaataca ctggtagcaa gggcaatctc agaggtgata
     4441 tcaactatca aagaggtggc agaaaataaa gaggctacaa aagcagaaat aataaaggtg
     4501 atgtagtggt taaacttgtt tttctgcatt tgtaacttta caaaaaagcc tgcaattgta
     4561 gctgagaaaa caatagccat cgaaatccag tcgccaccaa ataaacggca gaatgatgca
     4621 ttggcaaagc ccaccagcaa caatacaaac agagtatcca tttgtggcga ttctactatt
     4681 ttttgatatt tcttttttgt ctcttcaaaa gtcaatcgat tgtcaacagc ttcccagctt
     4741 aatgcactga gttcagaatt ttgttcgaaa ctaatgggaa tgacaggaat ttcatttact
     4801 tcactgtaag tcttctgagt ttcaatatcg agcatagtca gaataaggct tttttgaaag
     4861 acgcttattt gaacgtcaaa atccaatgat tctccaatac gtttcgtatt acgcactacc
     4921 ctggatgtat gcacaccaca acccatcaaa tgagttgcat attcggctat gaattttgtt
     4981 tgcgatacga tggtttcatt tgtcttcata tttttcaaac taagggctat tcgatgaata
     5041 agtttactag tccgaatagt aaaccgggac tttttttatt ctgaaatctc ttttattaat
     5101 ttatcagtga gatatttcta acacgtacgg cattaactcc atagtcaagc ggctgggatg
     5161 cgaacagata atgtccggaa gggtcatacg ctaacgatga aacggcatcc accacagtag
     5221 tctgattctg cccgttaacg tttacatcca gcattaccct gatggttttt gctgaaaccg
     5281 gaattcctga gtttttatta accttcacaa gtagctgctt aaagccctgc tgcgaaggaa
     5341 caagttcaat ttctttcaca ggcactccgc ccattgtctg attacttatc aacacctgac
     5401 tgatgccatc attttcaggt ggatggttgt aaaagtatcc atcatagcca taaaacagtt
     5461 ttttccattc gatatccttg ctgcggtcag gtaacacgcc ggcaagatta ctctgaataa
     5521 aggatgccag tttgtaggtg gatacaagcg aaccgtaata ggtcatgtgc tgattttcct
     5581 tcaccgtatt ttcaaaacat tcgggagtaa acgctttata atcgtaaggt gactgctgat
     5641 tgtcccacaa ctgcttttgt ggctcaagca cctttgccac ctttgccaca taggcatcca
     5701 tgtccttaaa atgacgataa tacatgggta aggtcaggaa aacaagtttc acattattct
     5761 ccttgcaaag ttgaaccgtt ttagtcacaa attcttcggc ttcgctgctg atgatgaatt
     5821 tcttaccatc aaaccctacg acttcattgt acttcatcag cgtactgtct tccagtccgg
     5881 aagtaaaacg gatataccgg cccagataaa gtccctgttg ttcatcctgc ttcaccttat
     5941 tcaactgtat atttgtttcg atctgtttga aatccgaaaa tataaaatta tgattgcgta
     6001 ccgtgttcga ccaggccgaa aggtagtttc ttgagttgaa aagcacgggg gtagagcata
     6061 gtttttgtcc caggtttttt cttgcggaga aactcttgaa ttgatcggac agcgttccct
     6121 gcttaagttc gtgcgatgta taatcattga taccaaacgt
//
LOCUS       DAOPYB010000179         6135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_15855_length_6135_cov_7.902961, whole genome shotgun sequence.
ACCESSION   DAOPYB010000179 DAOPYB010000000
VERSION     DAOPYB010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15855_length_6135_cov_7.902961"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..651
                     /locus_tag="PKH58_11135"
     CDS             1..651
                     /locus_tag="PKH58_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNX89629.1"
                     /translation="MIIIEIIFFSCGKKERNESKEKTVVTDTVMNEKSVEIPDCNYTF
                     EEAIAGSEAPEKILKELVLLDVKYYSTDNKIHRGQVVVNRKIAQDIVEIFEFMLKEHF
                     PVYQVVPIVRYDWDDKKSMAANNSSGFCYRNVSNSSKKSKHSTGMAIDINPFFNPIRY
                     KSPYQHKPNVPEGAKLDINVPGTLTSDHPVVRKFQQLGFRWGHRFKKYFDDHHFDK"
     gene            758..3061
                     /locus_tag="PKH58_11140"
     CDS             758..3061
                     /locus_tag="PKH58_11140"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNX89630.1"
                     /translation="MAKLTKEDALLYHSQGKPGKIEVVPTKPYSSQRDLSLAYSPGVA
                     EPCLEIEKDNSNAYDYTSKGNLVAVISNGTAVLGLGDIGAVAGKPVMEGKGLLFKIFA
                     GIDVFDIEVDEKDPEKFIQVVKAIAPTFGGINLEDIKAPECFEIEERLKEELKIPVMH
                     DDQHGTAIISAAGLINALEIIGKKIEDVKIVVNGAGAAANSCTKLYMALGAKLENIVM
                     VDSKGVINTKRTDLNNRKKPFATSRNIDTLAQAVEGSDVFLGLSVADVLTPEMVQSMN
                     ENPIVFALANPNPEIAYEVAMSARPDIVFATGRSDHPNQINNVLGFPYIFRGALDVRA
                     SAINEDMKVAAVKAIAELAKKPVPDVVNSAYNMKKISFGRDYIIPKPLDPRLLTTVAP
                     AVAKAAIDSGVAAKKIEDWDAYEVQLRKLMGLDNKMLRRFYDTARQNPKRVVFSESLH
                     INMLKAAETALAEGICIPILLGNEEKLANVAAENGIDLTGIQIVNLRHDREEERRIRY
                     AKLLAKKRSREGMTFEEAFEKMYERNYFGMMMVETGEADALITGVITKYEDSVKAAKT
                     VIGARPGVNNIGAMHIMNTKKGTYFLADTLFNRHPSTEVIIDVAKLTYDTVKFFAHEP
                     VMALLSYSNFGSDIEGSPASVHKVNQVLQERYPDMVVDGEMQANFALNRELRDRKYPF
                     SRLKGKDVNTLIFPNLSSANMVQQMMLELGLAETIGPIQMGLSKPVHILDVESSVRSI
                     VNMTAVAVLDAIVQEKIEKGEVQRVGE"
     gene            3410..4834
                     /locus_tag="PKH58_11145"
     CDS             3410..4834
                     /locus_tag="PKH58_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HNX89631.1"
                     /translation="MNNLDYETLNVEDTTSRDSVQKIVGRQPWRVIALDNLCNFSVSG
                     FDLFILNADFAKVNLITIRKNLNQLPVWDTDEFSTNFFWHPYHGSVLHNSARMYGYNF
                     YETIPFNFLGSLSWEYFHENEPPSANDLITTTIGGSSLGEMTFRISDMILDNRSIGFE
                     RVGREVIAGLLSPSRFLTRLVSGKAWTFSRKKGNLLPTEPVDFEFDTGIIYLREKSGR
                     NDVGVLARGQLEYGDILKNTIRKPYEWMLLRGQFELLSGKIRASQICGIGALRVHNLL
                     SVRNTSLSAGLFQHFDYYNLKLNMNDGSVLTPFYISEAAAIGPGLIWYRKYLNTEMKV
                     RLYANGIGLGASMSDYFNFDDKDYNTGSGFGFKFFYEFIPSRKLIFSLNAENYQIYTW
                     KGAAKSLYSMKLTKAEIMQLNIQGDQSKTSLNLIGLKINYQLSPRTSISLQGRQYHRH
                     THYVFFDEMDFSCTEHNISINYSF"
     gene            complement(4912..5667)
                     /locus_tag="PKH58_11150"
     CDS             complement(4912..5667)
                     /locus_tag="PKH58_11150"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNX89632.1"
                     /translation="MDRIIKKYYSLVKKFQKHDKDISEKGIHKKRIILCKVRSILSAL
                     DIKDKKIRNGKKAFRLMGNIRNIQIQIVILQPSEKEKEISRYLDYLDKKQNKSRSKYY
                     TFIKQKKIRFPTINRHHNINIDNIKSQIALLHDNLIQNISSIIVYDAAAIHKIRKSFK
                     IFRYMIENLSIVIDIDKNVIDKLKEFQDKLGEIQDYSMLTKGIKEFNRENKNSPIDYF
                     QFENYLNKLISNFSEEIDSLIAFCNQTRQLTSE"
     gene            5826..>6135
                     /locus_tag="PKH58_11155"
     CDS             5826..>6135
                     /locus_tag="PKH58_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89633.1"
                     /translation="MKSKFRIFLLPILIVFPLVVSAQNKVTEKIMLMGKTDNRTMQHL
                     DILSNRIGARPIGSDAYENATYWVAAQLRKWGLDVEIQEVGELPVGFNRGPWFGRMYS
                     D"
BASE COUNT         1931 a         1040 c         1252 g         1912 t
ORIGIN      
        1 attataatta ttgaaataat atttttttca tgtggcaaaa aagagagaaa tgagagtaaa
       61 gagaaaactg tggtcacaga tacagtcatg aatgagaaat ctgtagaaat tccggactgc
      121 aattatacat ttgaagaagc aatagcggga tctgaagcac ctgaaaagat tttaaaagaa
      181 ttagttttgc tggatgtaaa gtattattca acagataata agatacatag aggacaggtg
      241 gtcgtaaaca ggaaaattgc tcaggatatt gtcgaaatct tcgaatttat gctaaaggaa
      301 cactttccgg tttatcaggt tgttcccatt gtcagatacg actgggatga taaaaaatca
      361 atggccgcca ataattcctc cggtttttgc taccgaaatg tttcaaacag ctctaaaaaa
      421 tcaaagcatt ccaccggaat ggctattgac atcaatccct ttttcaatcc tatccgatac
      481 aagtcaccct atcaacacaa accgaacgtt cctgagggcg ccaaactgga tataaatgta
      541 ccgggaacac taacatccga ccatcccgtg gttaggaaat ttcaacaatt aggcttcagg
      601 tggggacacc gttttaagaa gtactttgat gatcatcatt tcgataaata attgttaaaa
      661 taacgaatag gtttcattac gaaatgaaaa ataaagagta cctttgcggg aaatatcaaa
      721 agtaaaaata ctgaataaat aacaatataa aataataatg gcaaaattaa caaaagaaga
      781 tgctctgcta tatcattcgc agggcaagcc gggaaaaatt gaggttgtac ccaccaaacc
      841 ttacagttct cagcgcgatt tatcactggc atactcgccc ggagtagccg aaccgtgtct
      901 tgaaattgaa aaagataatt caaatgcgta cgattatact tcaaaaggta atctggtcgc
      961 tgttatttca aatggtacag ctgtacttgg tttgggtgat atcggagctg tggccggcaa
     1021 accggtaatg gaaggtaaag gattactatt taagattttt gcaggtattg atgtgtttga
     1081 cattgaagtt gatgaaaaag atccggaaaa atttatacaa gtagtgaaag ccattgcacc
     1141 gacgtttgga ggtatcaatc tggaagatat caaagcgcct gaatgttttg agattgaaga
     1201 aaggctgaaa gaagaactca aaattccggt aatgcatgat gatcagcacg gtacagccat
     1261 tatttcagct gcaggtctta tcaacgcgct tgaaataata gggaagaaaa ttgaggatgt
     1321 aaaaattgtg gtaaatgggg ccggagctgc agctaactcc tgtacaaaac tatatatggc
     1381 gcttggggct aaactcgaaa atatcgtaat ggttgattca aaaggtgtaa taaatacaaa
     1441 acgtaccgat ttgaataacc ggaaaaaacc ttttgcaacg tcacgaaata tcgataccct
     1501 ggctcaggct gtagaaggtt cggatgtttt tctgggttta tctgttgcag atgtgctgac
     1561 ccccgaaatg gtacaaagta tgaatgaaaa cccgattgtg tttgctttgg ctaatccaaa
     1621 tccggaaatt gcctacgaag ttgccatgtc agctcgtcct gatattgttt tcgcgaccgg
     1681 acgttccgat catcctaatc agattaacaa tgtgcttggc tttccttata ttttccgtgg
     1741 tgctcttgat gtacgtgcca gtgccatcaa tgaagatatg aaagttgctg ccgttaaggc
     1801 aattgctgaa cttgcaaaaa aaccggttcc ggatgttgta aattcagctt ataatatgaa
     1861 gaaaatcagt tttggccggg attatattat tccgaaaccg ctcgatccca gattgctcac
     1921 aaccgttgct ccggcagtgg caaaagctgc cattgattcg ggagtagctg ctaagaaaat
     1981 tgaggattgg gatgcatatg aggtacaact tcgtaagtta atggggcttg acaacaaaat
     2041 gcttcgccgg ttctacgata ctgccagaca gaatccaaaa cgggttgtat tttcagaatc
     2101 attgcatatc aatatgctca aagctgctga aacagctctt gcagagggta tttgtatccc
     2161 gatattgctt ggtaacgaag aaaagcttgc aaatgtggca gccgaaaacg gtattgacct
     2221 gactggtata caaattgtta atctccgtca tgacagagaa gaggaacgcc gaattaggta
     2281 tgcaaaatta cttgccaaaa aacgcagtcg tgaaggaatg acttttgagg aagcgtttga
     2341 aaagatgtat gaacgcaact attttggaat gatgatggtt gaaaccggag aagcggatgc
     2401 gctcattaca ggtgttatta caaaatacga agattcggtg aaagcagcga aaacggttat
     2461 tggtgcccgt cccggagtaa acaatattgg tgccatgcac atcatgaata ccaaaaaggg
     2521 aacctacttc cttgctgata cattgttcaa tcgtcatcca tcaaccgaag ttattattga
     2581 tgtagccaaa ctgacttacg ataccgtgaa gttctttgca cacgaaccgg tgatggcgtt
     2641 gctttcatat tccaattttg gttcagatat agaaggtagt cctgcaagtg ttcataaagt
     2701 gaatcaggtg ttacaagaac gctatcctga tatggtggtc gacggtgaaa tgcaggcgaa
     2761 tttcgctttg aatagagaac ttcgcgatcg taaatatcca ttctcccgtt tgaaaggtaa
     2821 agatgttaac acgttgattt ttccaaatct tagctcggca aacatggtac aacaaatgat
     2881 gcttgaactg ggattagcag aaacaatcgg accaatccag atggggctga gcaaaccggt
     2941 tcatattctg gatgttgaaa gttctgttcg cagcatagtt aacatgactg cagtagctgt
     3001 gcttgacgcg attgttcagg aaaagataga aaagggtgaa gtgcagcgtg ttggcgaatg
     3061 aaatattaac tgaaaataaa taataagaac ggggttagtc gtaagattaa ctccgttttt
     3121 ttttgaaata atatatctgc ttattaattt tttgttggtt atgaattaat aacttgtctg
     3181 caaatgctga aaatcaatga gttatttctc aattagtttg ttaaactagt ctgtaatata
     3241 tttgttatat attatataat taaattttgg ttagatccaa ataaccgaaa tgtatatact
     3301 gattttcgct tgtattaatg gtgcagtttg tcgcatataa ttaattgcag tgaaagggat
     3361 tataacattt atctgttttg tttacttatt catcctgcca atacgatcaa tgaataacct
     3421 ggattatgaa accctaaacg tagaagatac taccagtagg gattcagttc agaaaattgt
     3481 tggcagacag ccctggagag taatcgctct cgataacctg tgcaatttta gcgtatccgg
     3541 ctttgatttg tttattttga atgctgattt tgccaaagtt aatctgatta caatccggaa
     3601 aaatctaaat caacttccag tctgggatac cgatgaattt tcaacaaact ttttctggca
     3661 tccctaccat ggttctgtac tccataacag tgcccgtatg tatggataca acttctacga
     3721 aactattcct ttcaattttt taggcagcct ttcgtgggag tattttcatg aaaacgagcc
     3781 tccttcagct aatgatctga taactacaac cattggcgga tcatcgctcg gagaaatgac
     3841 tttcagaata tcagacatga ttctcgataa ccgttcaatc ggttttgaaa gggttgggag
     3901 ggaagtaatt gcaggtttgt tatctccttc acgtttttta acccgacttg tttcgggaaa
     3961 agcatggact tttagtcgca aaaaaggaaa tctcttaccc acagaaccgg ttgattttga
     4021 atttgacacc ggtattatat atttaagaga aaaatcaggt cgtaatgatg ttggagtgct
     4081 tgccagagga cagcttgagt atggcgatat tctgaaaaat acaatcagaa aaccttatga
     4141 atggatgctt ttgagaggac agtttgaatt attgtcaggt aaaatcaggg ccagccaaat
     4201 ctgtggaatt ggtgcgctga gagttcacaa tttactttcg gttagaaata caagtttgag
     4261 tgccggttta tttcagcatt tcgattatta caatctgaaa ttgaacatga atgatggttc
     4321 ggtactcact cctttctata tttcagaagc cgctgcaata ggacctggcc ttatttggta
     4381 cagaaaatat cttaatactg agatgaaagt cagactttat gcaaatggca ttggtttagg
     4441 agcaagtatg tcagattact ttaactttga tgataaggat tacaacacag gcagcggttt
     4501 tggatttaag tttttctatg aatttattcc gtctagaaag cttattttca gcctgaatgc
     4561 tgaaaattac cagatttata cctggaaagg ggctgcaaaa tcgttatatt cgatgaaact
     4621 taccaaagca gaaatcatgc aactaaatat acagggtgat caaagtaaga ctagtcttaa
     4681 tttaattgga ttgaagataa actatcagtt aagtccacga accagtattt ccttgcaagg
     4741 ccgacagtat caccggcata cacattatgt tttttttgat gaaatggatt tttcttgtac
     4801 cgaacacaat attagcatca actattcatt ttgataatag gatgaaaaat ctgttgtacc
     4861 tcaaggcaat ctgctttaca cagactttct gagattgtta catactgatg tttattcaga
     4921 tgtaagctgt ctggtttggt tacagaaagc tattaagctg tcaatctcct ccgaaaaatt
     4981 gctgatcagt ttatttaaat aattttcaaa ttgaaaatag tcaatagggg agttcttgtt
     5041 ttctcggtta aattctttta ttcccttagt taacatacta taatcctgta tttcacccaa
     5101 tttatcctga aattctttta gtttgtcaat aacattcttg tcaatatcga ttacaatgga
     5161 aagattttca atcatatagc gaaatatttt gaatgacttc ctgattttat gaattgcagc
     5221 ggcatcatac actattattg aagaaatatt ttgtatcaga ttgtcgtgca gcaaagctat
     5281 ttgtgatttg atgttgtcaa tgttgatgtt atgatgcctg tttattgtag ggaaacggat
     5341 ttttttctgt ttgataaacg tataatactt gcttctggat ttattctgtt ttttatcaag
     5401 ataatcaagg tatctactta tttctttctc tttttcggac ggttgcagta tgacaatctg
     5461 aatttgaata tttcggatat tacccatcag acgaaatgcc tttttcccat tcctgatttt
     5521 tttatcttta atatccagag ccgacaatat ggaacgtact ttgcaaagta tgattctctt
     5581 tttatgaatc cctttttccg aaatatcttt atcgtgtttt tgaaatttct taaccagcga
     5641 ataatatttc tttataatcc tgtccattta gatcaactgt gaagttcgta tatctacaaa
     5701 acaaagtttt aattcatata gtttgatttt atcctgatgg tgttaaaaat ttaaagatta
     5761 aattaagata tattctcacg ttttattacc tttgttaaat cgtttaaaaa aatactcaga
     5821 taaatatgaa aagtaagttt aggatttttc tattacctat actgatagtt tttcccttag
     5881 ttgtaagcgc tcaaaacaaa gtaaccgaaa aaataatgct gatgggaaaa accgataatc
     5941 gtaccatgca gcatttggat atactcagca accgcatagg ggctcgtccc attggatctg
     6001 acgcttatga aaatgctaca tactgggtcg ctgcgcaact ccggaaatgg gggcttgatg
     6061 tcgaaattca ggaggtgggt gagttgccgg ttggatttaa ccggggaccc tggtttggaa
     6121 ggatgtacag tgatg
//
LOCUS       DAOPYB010000180         6112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_15937_length_6112_cov_6.740300, whole genome shotgun sequence.
ACCESSION   DAOPYB010000180 DAOPYB010000000
VERSION     DAOPYB010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15937_length_6112_cov_6.740300"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PKH58_11160"
     CDS             complement(<1..847)
                     /locus_tag="PKH58_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HNX89634.1"
                     /translation="MKSLNFDTEKIQVLDFETLKATYKENDVYGNPLRDVYHYQVISN
                     ILDICKANGLRYDIQDMFAANNNSRQFPGVSILPQVASIHGENSPQSHILRRVYTTIA
                     IHDGEDDELTTNIAIAYHQDGLQVAFGTTVKICHNLCILGADKMITNYGKSGKSNDEI
                     FSSVDDWLKNFGNYQNRDLEFIRKMRQIDVSKADALRFIGTLTAIRVAYDNGIGEVRQ
                     SLNTYPLNQAQISIFTENYLRFEQENENVTLWDLYNMGTHIYKPHTTEIPNILPQTAV
                     LFQVLN"
     gene            complement(849..1181)
                     /locus_tag="PKH58_11165"
     CDS             complement(849..1181)
                     /locus_tag="PKH58_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89635.1"
                     /translation="MKTNEAIKAKRQEIKNISLTIRELKENGSIQTINEGLKEIYKQQ
                     GNADLRTFEQWKKAGFQIKRGAKALYLWGRQTAKTVNEDGEEKEITYFPLVAVFSENQ
                     VYNSFNNQ"
     gene            complement(1259..1654)
                     /locus_tag="PKH58_11170"
     CDS             complement(1259..1654)
                     /locus_tag="PKH58_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89636.1"
                     /translation="MKANSSAPKLEQANEVQALRILPKETATEVKPTEVKPVEEVKPL
                     TIEDIKRKNEVVKRLTDKYDRLNEKLRRVQNFKLSSDRETATILVTDSNGEEFQSNSQ
                     KTMVQLIAFWHEEFKEAIAEVEREIRENA"
     gene            2377..2625
                     /locus_tag="PKH58_11175"
     CDS             2377..2625
                     /locus_tag="PKH58_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89637.1"
                     /translation="MNRIHQSKIWSDIEQKDKRGNYISFSFSYAKKDGTIAEYSNAVL
                     SSIHYKGSTINLMLPGEVKPHTFRRICFLTFNGKRTYL"
     gene            2625..4022
                     /locus_tag="PKH58_11180"
     CDS             2625..4022
                     /locus_tag="PKH58_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89638.1"
                     /translation="MKKKDQEVFDFGSTVYLAGGNAIIEFTDSKEMVGDSSTPTESIT
                     PFNKKNIQVKFVKRGTSNKQPVDVMDKIYANSTLGANIAFNAKMGYGDGIMVVKKERQ
                     EDGSIKAIEQLPSEQPEIFKFLLDNNYINSVQEWAMDLVTFYESYAEFIFGTGSDKIV
                     QLNPVESVNSRLSEANDTGDIEYLGYSLRWHEGNPDDVTITPALNRRRPLMDLKIRRG
                     LELNLKGKKQKDNWNSYILQLMQPTPGRYYHGKPYWWAIFESGWYDFAVAIPKFKKAL
                     LKNQMTLKYHVKINREFWTKLFTQEGIDIANKEQVKARKKKFFNQMNDFLASEENAGA
                     SFVSHFEYDRVKNYEVHDIIISVIEASKTGGEYLEDSSEVTNVICYAMEIQPSLIGAN
                     SKNGSINGTEARELFIIKQAMMKPIRDLLVLPLYIVKEINKWDPDVHFVIPNIMLTTL
                     DKNTGAEKSIGNQKI"
     assembly_gap    4089..4188
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4189..5115
                     /locus_tag="PKH58_11185"
     CDS             <4189..5115
                     /locus_tag="PKH58_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89639.1"
                     /translation="IIQNIEQLKKLVKINSSIPFSSVSPFLNTAQEIYLVRYLGRELL
                     TKINDENMPEKYIELCNKAASAEGLLAMWLGNAELSVRISDSGFTVERTDTLAPASDS
                     KIAEVKQSLCMRAFQHLDSLLEYLEDHVVEFPEWALSRYYSGTVDNYIRSARQFQDTG
                     SVNIQYSLLRFEEMRPLMRQVQDRYVRELLGEELDTQLRSKENITDEHERKLFDYACK
                     FIANKTAELYTSENSIRNKSSIDRTTYDQHTRPVYFDLQNTGNFYADQAQFYYGKLER
                     AYNDYLVSAGKTPASEALDWNTDEKKLFIDIG"
     gene            5121..5930
                     /locus_tag="PKH58_11190"
     CDS             5121..5930
                     /locus_tag="PKH58_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89640.1"
                     /translation="MTRIDIENRTFEVPDSMNEMTVPQLVFLSELISEVVPVQAVKVK
                     MLIFCIGGRVKPMKYPEYHRIQVGRDVFALTTDEIVQASAAFDYLFTKPDEDGNCFLD
                     NRLTMPPVPFIRSGRIKLHAPGEALTECTYNQYIYLQTYDVMKDKNPVALYNFLGCMF
                     RRDLNRFNPEDLNIRTMKKIKSAEVVLMMWYWIGSCRYIADKFPRIFQADDAPPADGN
                     PYDGQQRLLDFMTRADAAKKQEYKRMKLYDVLFSLDFLLEQDEARREAVNQ"
BASE COUNT         1755 a         1247 c         1219 g         1791 t
ORIGIN      
        1 cattcagtac ctgaaataat actgccgttt gtggcaggat gttcgggatt tcggttgtgt
       61 gtggtttgta aatgtgtgtt cccatattgt acaaatccca aagtgtgacg ttttcatttt
      121 cctgctcaaa tcgcaaataa ttttctgtaa agattgaaat ttgcgcctgg ttcagtgggt
      181 aggtgttcag gctctgccgt acttccccga ttccgttgtc gtatgcgact cgtattgcgg
      241 tcagcgtacc aataaatcgg agtgcgtccg ctttgcttac gtctatctgt cgcatcttgc
      301 ggataaactc taaatctcgg ttctggtagt taccgaagtt tttcagccag tcgtcaaccg
      361 aagaaaaaat ttcatcgttt gattttccgc ttttgccgta attagttatc atcttgtcag
      421 ctcccagaat gcaaaggtta tgacagattt ttacggtcgt tccgaaagca acctgtaaac
      481 cgtcctgatg gtaagcgatt gcgatattcg ttgttagttc gtcgtcctct ccatcgtgta
      541 tggctattgt cgtgtaaaca cgtcttaaaa tgtggctttg cggtgagttc tcgccgtgga
      601 tgctcgccac ttgtggcaga atcgacacgc caggaaactg tcggctgttg ttgttcgctg
      661 caaacatatc ttgtatgtcg tatctcaagc cgtttgcctt gcaaatatcc agaatgttac
      721 ttattacctg atagtgataa acatcgcgta aaggattacc gtaaacatca ttttccttgt
      781 aagttgcttt cagcgtttca aaatctaaaa cctgaatttt ctctgtgtca aaattgagtg
      841 atttcatatt actggttatt gaatgagtta taaacttggt tttcagaaaa aacggctaca
      901 agtggaaagt atgtaatttc cttttcttct ccgtcttcgt ttacggtctt tgccgtttgc
      961 cgtccccaca gatagagagc tttcgcacct ctcttaattt ggaaacctgc ctttttccat
     1021 tgctcaaaag tccgcaaatc tgcgtttccc tgctgtttat agatttcttt caatccctcg
     1081 ttaatagtct gaatactgcc gttttccttc agttcccgaa tggtcagcga aatgtttttt
     1141 atttcctgcc gttttgcttt gatagcttca tttgttttca tacatttgta aagttttgta
     1201 tcggtttcaa ttagtttgaa taacctccta cagttgccgc tgtaggaggt tttttttatt
     1261 atgcattttc ccttatttcc cgctcaacct ctgcaatggc ttctttgaat tcctcgtgcc
     1321 agaatgcaat taactgaacc attgtttttt ggctgttgct ctggaactcc tcaccgttcg
     1381 agtcggttac taaaatagtt gccgtttccc tgtctgaact taatttaaaa ttctgaactc
     1441 tccgaagttt ttcattaagt cggtcgtatt tgtcagtaag tcgttttaca acttcatttt
     1501 tccgtttaat gtcctcaatg gtcagcggtt ttacttcttc cactggtttt acttccgttg
     1561 gttttacttc tgttgctgtt tcttttggca gaatgcgtaa tgcttgaact tcgtttgctt
     1621 gctccaattt tggagcgctt gaatttgctt tcattttttt tggttttaat tagtttgaaa
     1681 atatttattt taaaaattaa gtgtatcggc tttgctacgc ttggcgctac cgatgtgaga
     1741 atgtaagaat gtcaaagaac tataacctat cgggtaaacg gtccagtccg tttttctgtc
     1801 ggttttccct ctgataaccg atgaacagat tttaacatat cgggctaatc gctcatcaac
     1861 cctatacaaa aatacaaaaa ttatttgaat tacacacact taaaacgctg aaaaacaatc
     1921 agttaaatat gcaaattcaa tatgtgttat taaaattaat cccgtttcgc tcctgcctgc
     1981 tctcattcac gttaaaaaaa acttttaatc tttcggaata aaatccccaa aaaaacgctt
     2041 taaaatcctc tgcatcaatc cgataaataa gcgtttgtta aataaatata ataaaaatta
     2101 cattcattcc actgaaattc gtcaaaattt tagcataaaa ttttggcagt cttacattta
     2161 agggtttcaa agggaattat tttttccctt tgtctgttat ggaacctcgc aacgccctgc
     2221 caagaacttg cttaagcaag tttgaaaaat acgcaatatg ccaaaacgcc ccctgttaac
     2281 ccgcgcgcat gcgtgcgacc tataacatta attacttgtc ctttcaattt tttgcgtaag
     2341 caaataaatt tgctgctgta attaaaaagt agcaacatga acaggataca tcaatcgaag
     2401 atctggagtg atatcgaaca gaaagacaag agaggtaact atatatcttt ctctttctcg
     2461 tacgctaaaa aggacggaac gatagctgag tatagcaatg cggtattgtc aagtattcac
     2521 tacaagggat cgactattaa tctcatgtta ccaggtgagg tgaagcctca cacattccgt
     2581 agaatttgtt tcctgacatt taacggcaaa agaacatatc tataatgaaa aagaaagatc
     2641 aagaagtatt cgatttcggt tctactgtat atctcgctgg tggtaatgct atcattgagt
     2701 tcaccgattc taaagaaatg gtaggagact catcaacacc aaccgagtcc atcacacctt
     2761 tcaataagaa gaatatacag gttaagttcg taaagcgtgg aacttcgaac aaacaaccgg
     2821 tagacgtgat ggataagatc tacgccaatt caacgcttgg tgccaacata gccttcaatg
     2881 ctaagatggg atatggagat ggcattatgg tagtgaagaa agaacgtcag gaagatggta
     2941 gtattaaggc tattgagcag ctgccttccg aacagccaga gatattcaaa ttcctgctgg
     3001 ataataacta tatcaactca gttcaggaat gggcaatgga ccttgttacg ttctacgaga
     3061 gttatgcaga atttatattc ggcactggat cagataaaat tgtacagctt aatcctgttg
     3121 aatcagttaa cagccgattg tctgaggcta atgataccgg agatatcgaa tatcttggtt
     3181 attctcttcg gtggcatgaa ggcaacccgg atgatgtgac tataactcct gcactcaacc
     3241 ggcgcagacc attgatggac ctgaagatcc gcagaggttt agaactaaac ctgaaaggta
     3301 agaagcagaa agataactgg aatagctata ttctccagct gatgcagcct acaccagggc
     3361 ggtattacca cgggaaacct tactggtggg ctatatttga atcgggttgg tatgattttg
     3421 ccgttgctat tccaaaattc aagaaagccc ttcttaaaaa tcagatgacc ctgaagtatc
     3481 acgttaagat taaccgtgag ttttggacta agctatttac tcaggaaggc atagatatag
     3541 caaataaaga gcaggttaaa gcccgaaaga aaaaattctt taatcagatg aatgatttcc
     3601 tggcatctga agaaaatgcc ggagcctcat ttgtttcaca cttcgagtat gatagggtaa
     3661 agaattacga ggtccatgat attatcatca gtgttattga agcatctaag actggcggag
     3721 aatatctgga agacagttct gaagtaacga acgttatttg ttacgctatg gagatacagc
     3781 catcactcat cggtgccaac agcaagaatg gaagcatcaa tggaactgaa gcccgggaac
     3841 tcttcattat taaacaggct atgatgaaac ctattcgtga cttactggta cttcctttat
     3901 atattgttaa ggaaataaat aaatgggatc cggatgttca tttcgtcatt ccgaacataa
     3961 tgctcactac cctcgataag aatacaggtg ctgaaaaatc aatcggtaat caaaaaatat
     4021 aaaagctatg tcaataatcc agaacattga acagctgaaa aagctggtta agataaattc
     4081 atcaatccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat aatccagaac
     4201 attgaacagc tgaaaaagct ggttaagata aattcatcaa tcccatttag ctctgtatct
     4261 ccattcttga atacggcgca ggaaatatac ctggttcgct atcttggtcg tgagcttctt
     4321 acgaagatca acgacgaaaa catgccggaa aaatacatcg aactatgtaa taaggcagca
     4381 agtgccgaag gcttactggc aatgtggctg ggtaatgctg aattatcagt ccggatatcc
     4441 gacagcgggt ttaccgtgga gcgtacagat actcttgccc cggcatccga cagcaagata
     4501 gcagaagtaa aacaaagcct ctgcatgcgg gcgtttcagc acttagatag tttgcttgaa
     4561 tacctcgaag atcatgtggt tgaattccca gaatgggcat tgagccgcta ttacagcgga
     4621 accgttgaca attacatacg ttcagcccgc cagttccagg acacaggttc agttaacata
     4681 caatacagcc ttcttcgatt tgaggagatg cgcccgctta tgcgacaggt acaggacagg
     4741 tacgttcgtg aattgttagg cgaagaatta gacactcaac ttcggtcgaa agaaaacatc
     4801 accgatgaac atgaacgtaa attatttgat tatgcctgta aatttattgc aaataaaaca
     4861 gcagagcttt acacctccga aaacagcatc cgcaataaat catctatcga ccgtactacg
     4921 tatgaccaac atacacgccc tgtgtacttc gacctgcaga ataccggtaa cttctacgcc
     4981 gatcaggcgc agttctatta tggtaaactc gagcgcgctt ataatgatta cctggtttca
     5041 gccggtaaaa ctcctgcatc ggaagccctg gactggaata cggacgaaaa gaaattattc
     5101 attgatatag gttaacggct gtgacacgaa tagatataga aaaccgcacc ttcgaagtgc
     5161 cggacagcat gaatgaaatg acagtacctc agctcgtgtt tctctctgag ctgattagtg
     5221 aagtggtacc ggttcaggca gtgaaagtaa agatgcttat tttctgcatc ggcggccgtg
     5281 ttaagccgat gaaatatcct gagtaccacc gcatacaggt aggacgggat gtatttgccc
     5341 tcacaactga cgagattgta caggcttcag cggcgttcga ttatctgttt acaaagcccg
     5401 atgaggatgg aaactgcttt ctggataacc gcttaaccat gcctccggtt ccattcataa
     5461 gatcaggacg aataaaacta catgcacctg gcgaagccct caccgaatgc acatataatc
     5521 agtacatcta tctgcagacc tacgatgtta tgaaagataa aaatcccgta gcgttgtata
     5581 acttccttgg atgtatgttc cgccgggatc tcaatcgctt caaccccgaa gacctgaaca
     5641 tccgcacgat gaaaaaaata aaatcggctg aagtggtgct catgatgtgg tactggatag
     5701 gaagctgccg gtatattgcc gacaaattcc cgcggatatt ccaggctgat gacgcaccac
     5761 ctgccgacgg caacccgtac gacggacaac aacgcctgct cgacttcatg acgcgggctg
     5821 atgcagccaa aaaacaggaa tataaacgaa tgaaactgta tgatgttctt ttctcgcttg
     5881 atttcttgct tgagcaggat gaagccagaa gagaagcggt aaaccaataa ctattacaaa
     5941 tcacggactg tacttcattt ttttttggat taaatttgtc atgtgagtaa gctctgctgt
     6001 gaagcagggc ttattttatg tttagaaatt gctaaacttt acaactccat acaactacat
     6061 acaagccggt acaagtttaa ggactaattt ttcttaattt accgaacctt ac
//
LOCUS       DAOPYB010000181         6093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_16024_length_6093_cov_9.339020, whole genome shotgun sequence.
ACCESSION   DAOPYB010000181 DAOPYB010000000
VERSION     DAOPYB010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16024_length_6093_cov_9.339020"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /gene="ppk1"
                     /locus_tag="PKH58_11195"
     CDS             <1..1524
                     /gene="ppk1"
                     /locus_tag="PKH58_11195"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNX89641.1"
                     /translation="GSEYSRFLELPDVDGNKYLMMLDDVIRLCMPQIFQSLGYDSFET
                     YTVKITRDSEMEIESELGEGIMEKVTKGIKSRRFGLPLRFVYDRQMPEEMKKYLLKKI
                     DFKKSGTIIPGGRYHNLKDFINFPVLRENLVYTKTEPVENQIIKHSNSVIKSIEKQDL
                     FLHYPYYSFSQYVQLLREAAIDPDIKSIKITLYRMAHRSKVAKALIFAARNGKQVTAV
                     VELRARFDEEHNILWSRQMEEAGVKIVFGVEGLKIHSKLTLISKKSGKKIAAVSTGNF
                     HEGNAAVYTDFTLLTAKNSITGEVERVFDFIGQPFQNQHFNHLLVSPQEMRKKLNQLV
                     NNEISNAKKGLPAYIYCKINHIADYKFIDKLYDAAATGVKIKLIVRGMCSMVTNQPKL
                     KGNIQIVSIVDKFLEHSRIFIFCNNEAEKYYISSADWMTRNLDQRIEVAVPVYDKEIQ
                     KELKTIVEYGLKDNVKARITNGEGENKLQEDELPPFRSQQELMKHYKTLEESRITGK"
     gene            complement(1578..1994)
                     /locus_tag="PKH58_11200"
     CDS             complement(1578..1994)
                     /locus_tag="PKH58_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89642.1"
                     /translation="MIIAVDFDGTVVTHEYPNIGKEIPFAIDTLKRLQTEGMHQLILW
                     TVREGDLLTEAVEFCRERGLVFYAVNSNYPETEQVDRESRKVSADLFLDDRNLGGIPD
                     WGVIYRMIMSGNPWTPLSADFMPDSPKPKKKGWFGK"
     gene            complement(2025..2498)
                     /locus_tag="PKH58_11205"
     CDS             complement(2025..2498)
                     /locus_tag="PKH58_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89643.1"
                     /translation="MERTIKNINLVYYMLYMTTVVVAVIIFFLTFSNAQTTHIDEKSQ
                     TGIIISSVYILYLLISIPYALWSFHKKTKKWQQIKDQYVKLVEYEKGSKLRLWIIGAG
                     LIAGIVLVYFMRSKSMIFCAAIAAIALYFTKPTLNKMISELKLDEDREDEASENN"
     gene            complement(2523..3143)
                     /gene="recR"
                     /locus_tag="PKH58_11210"
     CDS             complement(2523..3143)
                     /gene="recR"
                     /locus_tag="PKH58_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNX89644.1"
                     /translation="MNQQFSSLLLENAVNEFSRLPGIGRKTALRLVLHLLKQSETDVE
                     GFGNAMINLRKEIVYCKVCHNISDTEVCQICSNHGRDHQTVCVVENIKDVMSIENTQQ
                     YRGLYHVLGGIISPMDGVGPNDLEIESLVSRVQTGEIKEVILALSTTMEGDTTNFYIF
                     RKLSPSNVKITTIARGIAIGDELEYADEVTLGRSILNRIEFTNSFK"
     gene            3261..4706
                     /locus_tag="PKH58_11215"
     CDS             3261..4706
                     /locus_tag="PKH58_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNX89645.1"
                     /translation="MHSYTVILIIILYFGVLLLISHLISRKGSDNDAFFRANRQSPWW
                     VVAIGMIGTSISGVTFVSVPGMVREINFSYMQTVLGFFFGYVVIARVLLPLYYKLHLT
                     TIYTYLDERFGRQAYKTGASFFLLGKTIGAAARLYVVALILQTILFDAWGVPFWLTVC
                     CTVFLIWLYTNRSGIKTVIWTDTIQTIILIAALVLLVVEVAQNINLTHIELFKYLSIS
                     PKTDVFVFDDWHSKQFFWKQFLSGVFIPIVMTGLDQDMMQKNLTCKTLKDAQKNMYWY
                     GIAFVPINFLFLTLGAMLLMLATQNGITLPASSDNILTLFATQYLGNTVFVLFVVGII
                     AAAFSSADSALAALTTSFSVDILEVDKLGDNDKAVKIRKKIHIIIAIVFVVIILLFRE
                     INDKSVIDAIYTIVSYTYGPLLGLYAFGLFTRCKTNNKLIPYTAVISPVLCYFLNFIS
                     TKYWNYSFSYELLMLNGLITFAGLWLFRQKK"
     gene            4752..5348
                     /gene="yihA"
                     /locus_tag="PKH58_11220"
     CDS             4752..5348
                     /gene="yihA"
                     /locus_tag="PKH58_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNX89646.1"
                     /translation="MEIKSAEFVISNTDYNKCPEGTKPEYAFIGRSNVGKSSLINMLC
                     NKKGLAMTSSKPGKTLLINHFIINDDWYVVDLPGYGYASTGKKMREQLKNIIESYILY
                     REQLVNLFVLIDCRHEPQKIDLEFIEWLGENEIPFSIIFTKADKLPVTKLRDNIVLYK
                     TKLLENWEELPPVFITSSEKKNGRDEVLNYIEEINKSL"
     gene            5396..>6093
                     /locus_tag="PKH58_11225"
     CDS             5396..>6093
                     /locus_tag="PKH58_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX89647.1"
                     /translation="MLDIQKKLSKPFLALLALPATAVGFALSTQIAALSWILSNKYGL
                     KIDEVAFVWLAGPIAGIFGQVIVGLISDNVWFMGGRRRPFIMIGGMVSTITFFALPHI
                     KGISHITGISDIVIIASVIALFLDLSVNVTFNPARSIIADLTPEGKIRTSGYVWMQII
                     SGSFGVLAYFLSMLFGNELLLYIAAILVLVMAVFPILFIEEKKDIPELQVSENKLGVS
                     QIFKEIFPLYGFLT"
BASE COUNT         1784 a         1292 c         1229 g         1788 t
ORIGIN      
        1 ggatcggaat attcccgctt tctggaactg cccgatgtcg atggtaataa atacctgatg
       61 atgctcgatg atgttatccg tttgtgcatg cctcagatat tccaaagcct cggatacgac
      121 agttttgaga cttatacggt aaaaatcacc cgtgactcgg aaatggaaat agaatccgaa
      181 ctcggcgagg gcatcatgga aaaagtgacc aagggaataa aaagccgccg tttcggattg
      241 cccttacgct ttgtttacga caggcaaatg cccgaagaga tgaaaaaata tctgctgaag
      301 aaaattgatt tcaaaaagtc gggtaccatc attccgggcg gaagatacca caatctgaaa
      361 gacttcatta actttcccgt gctcagggaa aacctggttt acactaaaac cgaaccggtt
      421 gaaaatcaga ttatcaaaca ttcaaactct gtcattaaat caatcgaaaa gcaggatctg
      481 tttttgcatt atccttacta cagtttttcg cagtatgtac agttgctgcg tgaagctgcc
      541 attgatccgg acataaaaag catcaagata acgctctatc gcatggctca ccggtcgaaa
      601 gtggcaaaag cgctcatttt cgcggcacgc aatggcaagc aggttaccgc cgtggtggaa
      661 ttgcgtgccc gtttcgacga agagcacaac atactctggt caagacaaat ggaggaagcc
      721 ggggtaaaga ttgtttttgg tgtggaagga ctgaaaatcc acagtaaact caccctgatt
      781 agtaaaaaga gcggtaaaaa aattgccgct gtcagcacgg gtaattttca tgaaggcaat
      841 gctgccgtat ataccgactt tacgctgctc acagctaaaa acagtatcac cggtgaagtg
      901 gaacgcgttt ttgactttat cggacaacca ttccaaaacc agcatttcaa tcatttactg
      961 gtttcgccac aggaaatgcg taagaaactc aatcaactgg tcaacaatga aatatcgaat
     1021 gctaaaaaag ggctgccagc ctatatttac tgcaaaatca atcatattgc agattacaaa
     1081 ttcattgata aactttacga tgcagctgca accggagtaa aaataaaact gattgttcgg
     1141 ggtatgtgtt cgatggtgac caatcaaccc aaactgaaag gcaatattca gattgtaagt
     1201 attgtggata aatttctcga acattcccgt atatttatat tctgtaacaa tgaggcagaa
     1261 aaatactaca tttcttcggc cgactggatg acccgcaacc ttgatcagcg aattgaagtg
     1321 gctgttcctg tttacgataa agagatacaa aaggaactga aaacaattgt tgaatatgga
     1381 ctgaaagaca atgtgaaagc ccggattacc aacggagagg gtgaaaataa gttacaagaa
     1441 gatgaattgc cgccgttccg ttcacaacag gagctgatga agcattacaa aacactggaa
     1501 gaaagcagga taacaggaaa ataatgatgt acatcaataa aaaaagcccc gtattgcagt
     1561 ttcggggctt ttacttttta tttaccaaac caacctttct tctttggttt cggactgtcg
     1621 ggcataaaat ctgccgaaag aggtgtccat ggattaccgg acataatcat acggtatatt
     1681 acaccccagt cgggtattcc accaagatta cggtcatcca gaaataaatc ggctgaaact
     1741 ttacgggatt ccctgtccac ctgttctgtt tcgggatagt tgctgttcac ggcataaaat
     1801 accagtccgc gttcccggca gaactctacg gcttcagtca gcagatcacc ttcacgcacg
     1861 gtccagagta taagctgatg cattccttcg gtttgcaggc gtttgagtgt gtcaatggca
     1921 aagggaattt cctttccaat gttgggatat tcgtgtgtga cgacagttcc gtcaaaatca
     1981 acagcaataa tcattgttat tcattaagtt agaattataa tcaatcagtt attttcggat
     2041 gcttcgtcct ccctgtcttc atcaagtttc agttctgaaa tcatcttgtt cagcgtaggt
     2101 ttggtgaagt acagcgcaat agccgcgatg gctgcgcaga atatcatcga cttggaacgc
     2161 ataaaataca ccagtacgat tccggcgata agtccggcgc caataatcca caggcgtaat
     2221 tttgaacctt tttcatattc cactaatttc acatattgat ccttaatctg ttgccacttt
     2281 ttggtttttt tgtgaaaact ccacaaagca taaggtatgg aaatgagcag atacagaatg
     2341 tacaccgatg aaatgatgat accggtctga cttttctcat cgatatgcgt tgtttgagca
     2401 ttcgaaaagg tcagaaaaaa aataatgact gcaaccacta ccgtagtcat atacagcata
     2461 taatacacca gattgatgtt tttgatagta cgttccataa agttaatatt aagtttattt
     2521 gattatttga acgagttggt aaattcaatc ctgttgagga tggagcgtcc cagcgtaact
     2581 tcgtcggcat attccagttc atcgccgatg gcaattccgc gggcaatggt ggtaattttc
     2641 acattcgaag gtgaaagctt tctgaaaata tagaaattcg tggtatcgcc ttccatcgtg
     2701 gtactcagtg ccagaattac ctcctttatt tcaccggttt gcacgcggct taccagactt
     2761 tctatttcca gatcgtttgg tcctaccccg tccataggcg aaatgatgcc tcccagcaca
     2821 tgatacaatc cgcgatattg ctgcgtattt tcaatagaca ttacatcctt tatattttcc
     2881 accacacaaa cagtctgatg atcgcgcccg tgattactgc aaatctgaca tacctcggta
     2941 tctgaaatat tatgacacac cttgcagtac acaatttctt tccgcaggtt aatcatggca
     3001 ttcccaaatc cttcaacatc cgtctccgat tgcttgagca gatgcagaac aagccttaac
     3061 gccgtcttcc ggccaatgcc gggcagtctg gaaaactcgt tgactgcatt ttcgagcagg
     3121 agagatgaaa attgctggtt catttgaatg ctgagaatta ctttgcaaat ttagcaaaaa
     3181 aatcacattt ttactaattt agctgcaagc cttcgattta ccttaatttt cctacctttg
     3241 cattttctta gataattcaa atgcatagct acactgtaat ccttatcatc atcctttatt
     3301 ttggtgtact tctgctgatt tctcacctta tcagccgaaa aggttccgac aacgatgcct
     3361 ttttccgtgc caaccgccaa tcgccctggt gggtggtagc catcgggatg atcggaactt
     3421 ccatttcggg ggtgacgttt gtatcggttc cgggcatggt gagagaaatc aatttcagtt
     3481 acatgcagac tgtactggga ttttttttcg gctacgttgt cattgcccgt gtattgctgc
     3541 cactctacta caaacttcat cttaccacca tttacactta tctcgatgag cggtttggcc
     3601 gtcaggctta taaaaccggg gcatcgttct ttctgctcgg aaaaacgatt ggtgccgcag
     3661 ccagactcta tgtcgtcgcg ttgattttgc aaaccatcct gtttgatgcg tggggtgttc
     3721 cgttctggct aacggtttgc tgcaccgttt ttctcatctg gctttacacc aaccgcagcg
     3781 gtattaaaac ggtaatctgg actgatacca ttcagaccat tatacttatt gccgccctcg
     3841 tattactggt ggttgaagtg gctcaaaaca tcaacttgac tcatattgaa ctgtttaaat
     3901 acctttcaat cagtccaaaa acagatgttt tcgtttttga cgactggcac agcaaacagt
     3961 ttttctggaa acaatttctg agtggggtat tcattcccat tgtcatgacg gggctggatc
     4021 aggatatgat gcaaaaaaac ctgacttgca aaaccctgaa agatgctcag aaaaacatgt
     4081 actggtatgg tattgccttt gtgcctatca attttctttt ccttacactg ggagccatgc
     4141 tgctgatgct cgccacccaa aatggcataa cactgcctgc ctcttccgac aatatcctga
     4201 cactgtttgc cacacaatat ctgggcaata cagtatttgt actttttgtc gtcggaatta
     4261 ttgccgctgc cttttcgagc gccgactcag cactggcagc gttaaccacc tcattttctg
     4321 tcgatattct tgaagtggac aagcttggcg ataatgataa agccgtaaaa atccgcaaaa
     4381 agatacacat aatcatcgca atcgttttcg tggtcattat tttactgttt cgggaaatta
     4441 atgataaaag cgtgatcgat gcaatttaca ccattgtgtc ctatacatac ggtccattgc
     4501 ttggtttgta tgcctttggc ttattcaccc gctgtaaaac caacaataaa cttatcccct
     4561 acacggctgt catttcaccg gttttgtgct attttttaaa ctttattagc acaaaatact
     4621 ggaactattc gttcagttac gagttgctca tgttgaacgg attaattaca tttgcagggt
     4681 tgtggttatt ccggcaaaaa aaataataac attcaaacca tcaactttca taattcatac
     4741 ccctatcaaa aatggaaata aaatcagcag aatttgtcat aagtaatacc gattacaata
     4801 aatgtcccga aggaacgaaa ccggaatatg cgtttatcgg acgatccaac gtaggaaaat
     4861 catcactgat aaatatgctg tgcaataaaa aggggctggc catgacttct tcaaaacccg
     4921 gcaagaccct tctgattaat cactttatta tcaacgacga ttggtatgta gtggatttac
     4981 ccggatatgg ctatgccagc accggaaaaa agatgcgcga acagcttaag aatatcattg
     5041 aaagttatat cctttaccgt gaacagctgg ttaatctttt cgttctgatc gattgccgcc
     5101 acgagcctca gaagattgat ttggaattta tagaatggct gggtgaaaac gaaatacctt
     5161 tttccatcat ttttacaaaa gccgataaac tgcccgttac aaaattgagg gataatatag
     5221 tattatacaa gaccaaactg cttgaaaact gggaagaact gcccccggta tttattacgt
     5281 caagtgaaaa gaaaaacgga cgtgatgaag tactcaacta cattgaagaa ataaataaat
     5341 ctctctgata agtttcaatt aacatgaata ctaatcgtta aaaacacaaa agaagatgtt
     5401 ggacatacaa aagaagctca gcaaaccatt tcttgctttg cttgcacttc ctgctacagc
     5461 ggtaggattt gcgctttcta cccagattgc agctctgagc tggatattat caaataaata
     5521 cggattaaaa attgacgaag ttgcatttgt ctggcttgcc ggacctattg caggtatttt
     5581 cgggcaggtg attgttggtt tgattagtga caacgtatgg tttatgggag gcagacgccg
     5641 tccgtttatt atgataggcg gaatggtgag tactattaca ttctttgcac ttccgcatat
     5701 aaagggtata tcgcatatca caggcatttc agacattgta attatcgcct ctgtaatagc
     5761 tctattctta gatttatcgg tgaacgtaac gtttaatcct gcccgttcca tcattgccga
     5821 tttaactccc gaaggtaaaa tccgcacttc aggatacgtt tggatgcaga taatatccgg
     5881 ttcgtttggc gtgctggcat atttcctttc gatgcttttt ggaaatgaat tattgcttta
     5941 tattgccgcg atactggttc ttgtaatggc tgtttttcca atactattta ttgaagaaaa
     6001 gaaagatata cccgaattac aagtatcaga aaacaaatta ggtgtttctc aaatatttaa
     6061 agaaattttc cctttatatg gatttctcac ttt
//
LOCUS       DAOPYB010000182         6033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_16244_length_6033_cov_5.863499, whole genome shotgun sequence.
ACCESSION   DAOPYB010000182 DAOPYB010000000
VERSION     DAOPYB010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16244_length_6033_cov_5.863499"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..3172
                     /locus_tag="PKH58_11230"
     CDS             2..3172
                     /locus_tag="PKH58_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX89648.1"
                     /translation="MKKRLKMLLFLFLIGVGVGYAQTRVVGNVVDENGQSVMGASIQV
                     KGLSGTGTITDASGNFLLKVPSGYNTLTVTYIGMKKQEVAVSSSVRIILQSTSETLNE
                     VVVTALGIKKDRKALGYAVEDVKSEELMRNKQVNVINSLAGKIAGVNITQSSGAAGSG
                     AQIILRGGTSASESRDNQPLFVVDGVIYDNSSSVVGNSAFDGAGSSASTMSNRVMDIN
                     SEDIENISILKGPAASALYGSRASAGVVLITTKKGKEGAVEVNLSTKYVSSRAKYLPE
                     TQTKYKRGYMEDLYNTGGSYTGTVFNDYSYNSWGEKVSTSDPIYDNIGNFYQNGGVYD
                     TNLSISGGTKNGNFYLSGSNYDQDGIVPTTGYKKSTFRLNGEQKYGIFTFGANAAYSD
                     AKTQKTLTSAGLYNSQGTGALYAVNTWSPSDDMTHYINNDGTRYRMFGDLLDPWEERD
                     NPYWIINKNKLYDSTDRFTGNFNVKVDIAKWWWLSYRMGIDSYTTENHTRIAPEGAVK
                     LEWQNGMMSDNTLRFKYVSTNLMTNFNKKFGDFDLNLMLGTSTDNTHTNTHYEMAWNF
                     QVPNFYATDNATADNRNFKTYLSDKRLVGVFGEFRADWRNAVYFSVTGRNDWTSTLPI
                     ENRSYFYPSFNGSVVFTEFLPKNSWLSFGKLRVSQAKVGKDTNPYETTTPLWPVATYI
                     GGKVGVGNSWTRGNPYLKPEMTKSTELGLELRFLNNRMKMDATYYTNNSYNQILSPRG
                     PQSTGYIFTSINAGNVYNKGMELAISGTPVQNKDWNWETSINVAGNRGTMDGLLPGMD
                     IMYVTDVQYGNAKAASFSGGDFMAISGSQYSRTPDGEIILDKSGMPTYDKSTAHQVGN
                     REPKFTGGWNNTISYKNLTFNMLWEFRVGGDVFNGTKYAMTQSGTSKFSGDVRDHLVI
                     DGVENVGTADVPVYEVRHYEWFADQAYDFNGVKMSGANIIKNYYTGYYNYETANYITK
                     VNSLRLRTVSLGYDIPNTFLRKLKFIKRASLTASANNLLLFTNYEGDPEVAAAGSGRG
                     GSSSVGFDYCGVPATQSVSFGMNITF"
     gene            3207..5048
                     /locus_tag="PKH58_11235"
     CDS             3207..5048
                     /locus_tag="PKH58_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX89649.1"
                     /translation="MKNIIKSIIISLIGMFSFISCSDWLDVNVDPNTPTDQSATIENR
                     LPHIQFYANDAYQFASMRTFMAMGDMTMNSRTSTYGYAAQWQVSTSWVTTPYQWFFVG
                     AGSNLQALYEKAMKQEAWHYAAASRLIHAYGFMLMTDLYGEMPYNEGLGSSSTPKYDN
                     GKTIYLGCLTEIDEAIELLQKTQAVSVPSLTLGDTWANGDVSRWLKMAYLLKARWINK
                     LSKKQPGSYKEGKWDANEILACLDKAQKSNADNVFYDHTDDYGPSRDVLGWNEPVDYA
                     PLYSVLGMNSNYYVTQMMIDNFTNFAGSGIEDPRADHVIPWAYSVKGATSPTNLKWVG
                     NWRRTAGIDMHTLIRIQKHPYTTSYDAKKGGWYINTTDASLKGDTIYVQTTCGSKGYG
                     GFSSLLYEKATGDAKSRQSGIFHARPSSPTWIATYHEACFIKAEVKFKQGDKIGAFNA
                     YKDGIRANMEFMNQKLNVWISGDPSLVSCPSFTPMKQSDIDNYISNAIGTASDITLAK
                     IMTQKRMAMLWSLEVYNDMRRYDYDPNVFLNWQIPAEYYQNTTAQKYIPLGKQLRRWQ
                     QCSHELNYNSANLQAIGAEVPGANMSSEAWNKDDAVWSINVWWDTAD"
     gene            5399..5563
                     /gene="tnpB"
                     /locus_tag="PKH58_11240"
     CDS             5399..5563
                     /gene="tnpB"
                     /locus_tag="PKH58_11240"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HNX89650.1"
                     /translation="MNGDVYVFLNKSRTTMKLLHWERGGFVIYYKRMDQLSLNPCGSI
                     FLPNVLWLRP"
     gene            5841..>6033
                     /locus_tag="PKH58_11245"
     CDS             5841..>6033
                     /locus_tag="PKH58_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNX89651.1"
                     /translation="MKKLVLFAILFNLTAISFAQNERIIVGAERTKEYFPILKNKRIA
                     VFSNHTGMVGNKHTLDILLE"
BASE COUNT         1890 a         1074 c         1231 g         1838 t
ORIGIN      
        1 catgaaaaaa agattaaaaa tgttattatt cctgttcctc ataggagtgg gagtaggtta
       61 tgctcaaacc cgggttgtgg gtaatgttgt agatgaaaac ggtcaatcag tgatgggtgc
      121 atccattcaa gtaaaaggac tatctggcac aggtacaatc actgatgcta gtggtaattt
      181 cttattaaaa gtaccaagtg gttataacac acttacagtc acatacattg gtatgaaaaa
      241 acaagaagtt gccgtgagtt cttctgtgcg tataattctt cagagtacct ccgaaactct
      301 gaatgaagta gttgtaacag cattgggtat aaaaaaagat cgcaaagctt tgggttatgc
      361 cgttgaagat gtaaaatcag aagagttgat gcgtaataag caagtgaatg taattaactc
      421 gctcgcgggt aaaattgccg gcgtaaatat aacgcagtct tcaggtgctg ccggcagtgg
      481 tgcgcagatt attctacgcg gtggtacttc tgctagtgaa agtcgtgata atcaaccttt
      541 atttgttgtt gatggtgtta tttatgataa ctcatcgtca gtggtaggaa attctgcatt
      601 cgatggcgcg ggatcaagtg cttccacaat gtcaaatcgt gtaatggata tcaattctga
      661 agacatagaa aacatatcta tattgaaggg tcctgccgcc tctgcgcttt atggttcgcg
      721 ggcttctgcc ggtgttgttc ttatcacaac taaaaaaggt aaggaaggag ctgtagaagt
      781 aaatctttct actaaatatg tatcttccag ggctaaatat ctacctgaaa cacaaacaaa
      841 atataaaaga ggttatatgg aagacctgta taataccgga ggtagttata ccggcactgt
      901 atttaatgat tattcttata actcttgggg agaaaaggtg agtacgtcag atcctattta
      961 tgataatatt ggtaatttct atcaaaatgg aggtgtttac gatacaaatc tcagtatatc
     1021 cggcggaaca aaaaacggca atttctattt atccggttct aattacgatc aggatggtat
     1081 tgtcccaacc accggctata aaaaatctac tttccgttta aatggtgagc aaaaatatgg
     1141 cattttcacc tttggtgcca atgctgccta ttctgatgca aaaacgcaaa aaacgctaac
     1201 ttctgctgga ctttataatt ctcaaggcac tggagcgctt tacgctgtta atacatggtc
     1261 tccaagtgac gatatgaccc actatatcaa caatgacgga actcgttatc gtatgtttgg
     1321 cgacttgcta gacccatggg aggaacgtga taatccatat tggataatca ataaaaataa
     1381 attatatgat tcaaccgacc gttttacagg taatttcaat gtgaaagttg atattgcaaa
     1441 atggtggtgg ttatcttacc gtatgggaat tgattcctat acaacggaaa atcatacccg
     1501 aatagctccc gaaggcgctg tcaaattaga atggcagaat ggaatgatga gtgacaacac
     1561 attacgcttt aagtatgtat ccaccaattt gatgacaaat tttaataaaa aattcggaga
     1621 tttcgacctt aatttaatgc tgggtacatc aaccgataac acccatacga atacgcatta
     1681 tgaaatggcc tggaacttcc aggtgccaaa tttttatgct acagacaacg caacagcaga
     1741 caatcgtaac tttaaaacat acctgtcgga taaaagattg gttggggtgt ttggagaatt
     1801 tcgtgcagat tggagaaatg cagtatattt tagcgtaacc ggtcgtaacg attggacttc
     1861 tacgttacct attgaaaacc gttcttattt ctacccttca tttaacggta gtgttgtgtt
     1921 tactgaattt ttaccaaaaa attcctggct aagctttgga aaattacgtg tgtctcaagc
     1981 aaaggtaggt aaagatacca atccttatga aacaactact cccctttggc ctgttgctac
     2041 ttatattggt ggtaaagtgg gagttggtaa cagttggact cgtggtaatc catatcttaa
     2101 acctgagatg acaaaatcaa cagaacttgg tttggagtta cgttttctca acaatcgtat
     2161 gaaaatggat gcgacctact atacaaacaa ctcttataat cagattcttt cacctcgagg
     2221 acctcaatct actggttata ttttcacttc tattaatgcc ggtaatgttt ataacaaagg
     2281 aatggaattg gctattagtg gcacacctgt gcaaaataaa gattggaact gggaaacaag
     2341 catcaatgta gcaggaaatc gtggtacaat ggacggacta ctccctggta tggacattat
     2401 gtatgtaaca gatgttcaat acggtaatgc taaagcagct tctttctctg gtggcgattt
     2461 tatggccatc tccggttcgc aatattcacg tactccagat ggagaaatca ttcttgataa
     2521 aagtggtatg cccacttatg ataaaagtac agcacaccag gttggaaatc gtgaacctaa
     2581 atttaccgga ggttggaaca atacgatatc ctacaaaaac ttaacattca atatgttgtg
     2641 ggagttccgt gttggcggag acgtgtttaa tggaacaaaa tacgcaatga ctcaatcggg
     2701 tacaagcaag ttctctggcg atgtccgaga tcatctcgtt attgatgggg ttgaaaatgt
     2761 aggaactgct gatgttcctg tgtatgaagt acgtcattat gagtggtttg ctgatcaagc
     2821 ttatgatttc aacggtgtca aaatgagtgg tgcaaatatc atcaaaaact actataccgg
     2881 ctattataac tatgaaacag ccaactatat aactaaagtt aattcacttc gtttacggac
     2941 tgtttcatta ggttacgata tacctaacac attccttcga aaactgaaat ttattaaacg
     3001 tgcgtctcta actgcatcgg ctaataacct cttgttattt accaattacg aaggagatcc
     3061 tgaagtggct gcggcagggt cagggcgcgg aggttcatct tcagtagggt tcgattattg
     3121 cggtgtacct gcaactcaaa gtgtatcctt tggaatgaat ataactttct aaatatctta
     3181 caaaacaaca taaattttat attagtatga aaaatataat aaaatcaata ataataagtc
     3241 tgataggtat gttttccttt atatcctgta gtgattggct ggatgtaaat gttgatccga
     3301 acacacctac agaccaaagc gcaacgatag aaaaccgctt gccacatatt caattctacg
     3361 caaatgacgc ttatcagttc gctagtatgc gtacatttat ggcaatggga gatatgacaa
     3421 tgaacagccg tacttctaca tatggctatg cagcacagtg gcaggtttca acatcctggg
     3481 ttacaacacc ttaccagtgg ttttttgttg gtgcagggtc aaatcttcag gctctatatg
     3541 aaaaagctat gaaacaagaa gcctggcatt atgccgctgc ttcacgcctt atacatgcgt
     3601 atggttttat gttgatgact gatctttatg gggaaatgcc ttataacgaa ggacttggtt
     3661 caagttcgac tcctaaatat gataatggaa aaaccatcta cttaggttgt cttactgaaa
     3721 tagatgaagc tattgaactc ttgcaaaaga ctcaggctgt aagtgtacct tctcttacat
     3781 taggcgatac ttgggcaaat ggagacgtaa gtagatggtt gaaaatggct tatttgctaa
     3841 aagccagatg gattaacaaa ttaagtaaaa aacagcccgg tagttataaa gaaggtaaat
     3901 gggatgccaa tgaaatttta gcatgcttgg ataaagcgca aaaaagtaac gcggacaatg
     3961 ttttttatga ccataccgat gattatggac catctcgtga cgttctcggt tggaatgaac
     4021 ctgttgatta tgctcctcta tattctgtat tgggtatgaa ttctaattat tatgttactc
     4081 aaatgatgat tgataatttt actaattttg ccggttcggg aattgaagac cctcgtgcag
     4141 accacgttat tccttgggca tactcagtaa aaggtgcaac ttcacctaca aacttgaaat
     4201 gggttggcaa ttggcgccgt accgccggta ttgatatgca tactttaatc cgtattcaga
     4261 agcatccata tacaacctcg tacgatgcga aaaaaggtgg ttggtatata aataccacag
     4321 atgcatctct taaaggagac actatttatg tacagacaac ttgtggctct aaaggttatg
     4381 gtggtttcag cagcttgttg tatgaaaaag ctactggtga tgctaaatcc cgtcaatccg
     4441 gtattttcca tgctcgtcca tcttcaccta catggattgc aacctatcac gaagcttgtt
     4501 ttattaaggc agaggtgaaa tttaagcaag gtgacaaaat tggagctttt aatgcataca
     4561 aggatggtat cagagcaaat atggaattca tgaaccagaa attgaatgtc tggataagcg
     4621 gtgatcccag tttggtgtct tgtccatcat ttactccgat gaaacagagt gatattgata
     4681 attatatttc taatgcaatt ggaaccgcaa gtgatatcac tttggctaaa attatgactc
     4741 aaaagcgtat ggcaatgttg tggtcattag aggtctataa tgatatgcgt cgctatgatt
     4801 acgatcctaa cgtatttttg aattggcaaa ttcctgccga gtattatcaa aacactactg
     4861 ctcaaaagta tattccatta ggtaaacagc tccgacgttg gcaacaatgt tcacatgaac
     4921 tcaattataa ctctgcaaac ttgcaagcaa ttggtgccga agttcccgga gctaacatgt
     4981 cgagtgaagc atggaacaag gatgacgctg tatggtcaat caatgtatgg tgggatactg
     5041 ctgattaaag atgcatagtt aatcttttta gaatgaagga aatggttgta aactaatcat
     5101 ttccttcatt ctaaaaaggt gatggcaaat cggtaagcat cccaattccg ccgtaatgtg
     5161 tgccacgtag aaactctcga cctttgccag aaaaaaacgt atatatagtt tgaatgaagg
     5221 taatcggttt gtagtatgcc tgcaaggtgt agatttgcga aaaggtttag atggtttatg
     5281 cggtttaatc cgttatactc aaacctttca ggtttcaacc tgtttcacaa aacgattctc
     5341 atgaagcaaa atccgaaaag tagaatgtaa taagtatatt tatctttgaa ccccaccaat
     5401 caatggcgat gtttacgtgt ttctgaataa aagtcgcacg actatgaaat tgctccattg
     5461 ggagcgaggt ggtttcgtga tttattacaa acgtatggac caactatccc taaatccctg
     5521 tgggtcaata tttcttccga acgtgctttg gcttcgcccc taaaatgaat gcccaactta
     5581 tttgttatcg aaacatattt atattattgg gggactgagt gagtagtaat tgctttttta
     5641 tgtgtgaaaa taattaaata tttttggtac ttattttaca taaaatttgt ctttacatca
     5701 aaagaactta atcgctttca aattaacatt aatgtgcttt tactattagt aattttgtaa
     5761 atataatcta actttgtatt ttttttcaga cataatctct ttgccattaa acacctcata
     5821 ttcacttatt tactctcgtc atgaagaaat tagtattatt cgcaattcta ttcaatctta
     5881 cagcaattag ttttgctcaa aacgaacgta taatagtagg tgctgagcga accaaagaat
     5941 acttcccgat acttaaaaac aaacgtattg cggttttctc caatcacacc ggcatggtgg
     6001 gcaacaagca tacactggat atcttgcttg aaa
//
LOCUS       DAOPYB010000183         6010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_16329_length_6010_cov_9.378338, whole genome shotgun sequence.
ACCESSION   DAOPYB010000183 DAOPYB010000000
VERSION     DAOPYB010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16329_length_6010_cov_9.378338"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2365)
                     /gene="brxC"
                     /locus_tag="PKH58_11250"
     CDS             complement(<1..2365)
                     /gene="brxC"
                     /locus_tag="PKH58_11250"
                     /inference="COORDINATES: protein motif:HMM:NF033441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HNX89652.1"
                     /translation="MKQRELYLKDIDRPIEGVIKANDDRHLLQELEEYVLTNELLGIS
                     NRNKMLPGLFEELTKSNFNSSIWISGYFGSGKSHLLKMLSLVLENKEIKGVKCADVFA
                     KKAIDDFELERNIKKVATIPTESILFNIAAKSDGITSMGSSIDPVLSIFLKVFNELLG
                     YDPLNPEIAEVERHLESIGKYDFFKTEYQKRFNKSWEKGREAIFLNQHELAEIYAEIK
                     SISTDEANRFLDNLINNYKLDIEGFGELVTKYIKSKQPGFRLVFCVDEVGQFIAENVR
                     LMLSMQTIAETLSFKAQGQSFLIVTSQNDLNATIGDLNARQAHDFSRIQGRFSIKLPL
                     TSANADEVIQKRLLLKKDDSNKLLTDIYEKEQNNVKTLLKFNEGSRQYQTYRDSEHFV
                     NTYPFIPYQFDLFQAAIKALSDHNAFIGSQQSVGERSMLGVFQQVAKAYADKDVSNIV
                     SFARMYEGIKDVLQSNIQSDILQADRSIDTDLAKEVLKALFLVKYVKGFHASVGNVAI
                     LLLPKFDFNLAEFHKQVQEALNLLESQTYIQRSAGDLYEYLTNQEKDVENEIKSTDID
                     PAAPGELLASYLFDEILRDSKVKLDTNNQPYEFGKKLDDNVIGRDKDFYVNFITPLNA
                     NSVTTANINMWSAGRPNDLIVYLPEDKRLFDELRLIRKTEKYIQTTNSPALDATKQRI
                     LSEKAQQNQERKRTVLSQLKENIGDARMLLNGSDLTDIGSKDPKTRITQGVQQLIKTI
                     YTNLKMLTVDFTEAHLQKIVQSQDDVLFKDTLHEVEIEILNRIQRNKG"
     gene            complement(2367..>2864)
                     /locus_tag="PKH58_11255"
     CDS             complement(2367..>2864)
                     /locus_tag="PKH58_11255"
                     /inference="COORDINATES: protein motif:HMM:NF020329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HNX89653.1"
                     /translation="GELPFFIHSFPVSQQQVVNANIQSLVKRLQNDGIEILEINLYKL
                     CLDILKRANIFDQIVEQEVNMPKLRLNRVLVSNLNVETVIIPEIHKRHAESNAKIVFL
                     TGVGEVFPFIRSHSILNNTQTLVGNIPLVMFFPGSYNNKTLTLFELIKDENYYRAHNL
                     NDYKI"
     assembly_gap    2865..2964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2965..3470)
                     /locus_tag="PKH58_11260"
     CDS             complement(<2965..3470)
                     /locus_tag="PKH58_11260"
                     /inference="COORDINATES: protein motif:HMM:NF020425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HNX89654.1"
                     /translation="MMTTKKYTFSFTGASALIPETSVIAEEYNRLKDWNAVRKSLLDK
                     NSLNKIKQATFAREFREIKKRLSLLTSDQLNLLVQGSRDEAKSIILLSLVKTYPFIKD
                     FITEVLLIKYLIFDNLLTETDYNKFFNTKILSHNELNAITESTAKKVKQRVYTLLEQV
                     GLITQAKNG"
     gene            complement(3521..4024)
                     /locus_tag="PKH58_11265"
     CDS             complement(3521..4024)
                     /locus_tag="PKH58_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89655.1"
                     /translation="MDNKQRSSLFINEIHQSFEKKYPEKSGFVVFSKGKKNMEFRRNE
                     YLFDILIGKKEYLVSAHKHNIPYISKACLCIESEFAENNRESVIDINKLLLAHTDTRI
                     MILPMGPNILMNYLNPLKPMLKNFKNRLIIMFFPHPKHWTDHTKQEYRAFEFTPPDSF
                     KSIEFSF"
     gene            complement(4224..5570)
                     /locus_tag="PKH58_11270"
     CDS             complement(4224..5570)
                     /locus_tag="PKH58_11270"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNX89656.1"
                     /translation="MESKMISKSSFIRGMQCHKSLWLHLNQPEERDETSESQQQIFDI
                     GHNVGELAQQLFPGGIDASRGDHSLVHEAITYTAELINNGQTVIYEAAFSDGETLCYM
                     DILVKEPDGWAAYEVKASTQVKDYQVNDVAFQYFVITRSGLPLKRVSLVHVNNQYVRH
                     GDLDIRKLFAIEPMTERLLPMQNNIPVDLLSLQEMLKAGFMPEISMGSQCNNPFTCDF
                     LEFCQQSFPVEEDRSEGRPATRNQDELDQFLDELEYPLYFMDFETIQFAVPQFDESRP
                     YQQIPFQYSLHLQQSKGGPLTQFSFLGTPPTDPRPEFIQSLLSHLGSSGSIIVWNQTF
                     ENTRLKEIARDFPEYSSMIDPLFDRVVDLMVPFRKKYLYTPDMNGSYSLKAVLPALVP
                     DLSYSNLEIQEGGSAGLTYESLYNDTDPESIATKRENLLKYCELDTLSMVRILERF"
     gene            complement(5577..>6010)
                     /locus_tag="PKH58_11275"
     CDS             complement(5577..>6010)
                     /locus_tag="PKH58_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89657.1"
                     /translation="KYNPVRDQIIRNIDSAIEVQQKSKDVNKNKGIIDDIFFLLLTPK
                     IFRTESYGGNRKTSLDIFKPQRSRLYCYKMDEYKNPLNLKSALPHRNEISSEHWQRLA
                     RNIGWITFEDINRVAFDNDTMAPPEKEMILEFMNERNMIEK"
BASE COUNT         1649 a         1182 c         1157 g         1922 t
ORIGIN      
        1 ttcccttatt acgttgaata cgattaagta tttcaatctc tacttcatgc agggtatctt
       61 taaataaaac atcatcctgg ctttgaacta ttttttgcag gtgtgcctcg gtaaaatcaa
      121 ccgttaacat ttttaggttg gtataaatcg ttttgatgag ttgctggacg ccctgggtta
      181 ttcttgtttt cgggtctttg cttccaatat cagtcaaatc agatccattc agcagcatcc
      241 tggcgtcacc aatgttttct ttcaattggc ttaaaaccgt ccgtttcctt tcctggtttt
      301 gttgtgcctt ttccgacagg atacgctgtt tggtagcatc caaggcagga gaattggtag
      361 tttgaatgta tttttcggtt ttcctgatta acctcaactc atcaaatagt cttttatcct
      421 ccggtaaata aacaattaaa tcgttggggc gaccggcaga ccacatgtta atgtttgctg
      481 tggtaactga gttggcattt aatggagtaa tgaaattgac atagaaatcc ttgtcgcgac
      541 caattacatt gtcgtccagt ttcttcccaa actcataagg ctgattgttg gtatctaatt
      601 tgactttgga gtcccggagg atctcatcaa aaagatagct ggccaataat tcacccggag
      661 cagcaggatc aatgtccgtg ctcttaattt cattttcaac atccttctcc tggttggtaa
      721 ggtactcgta taaatcacct gcagatcttt gaatgtatgt ttggctttcc aataaattta
      781 atgcttcctg cacctgcttg tgaaattctg caaggttaaa atcgaatttt ggtaataaga
      841 gaatggcaac attaccaaca ctggcatgga agccttttac atacttcaca aggaacaagg
      901 cctttagcac ttctttcgcc agatcagtat caatgcttct atctgcctga agtatatcac
      961 tctgaatgtt gctttgtaaa acatccttta ttccctcata cattcgggca aatgacacga
     1021 tgttgctcac atccttgtcc gcataagcct ttgcaacctg ctgaaatacg cccagcattg
     1081 aacgttctcc aacagattgc tggcttccaa taaatgcgtt gtgatcggat aaggccttga
     1141 tagcagcctg aaagaggtca aactggtaag ggataaacgg gtaagtatta acgaaatgct
     1201 ctgaatcacg gtaggtttga tattgcctcg aaccctcatt aaacttcaaa agcgttttga
     1261 cattattctg ttccttttcg tatatgtcgg taagtaattt gtttgaatcg tcttttttaa
     1321 gcagcaaccg tttctgaatt acctcatcgg cattggcgct ggtaagtggc agtttaatag
     1381 aaaaacgtcc ttgaatacga gaaaagtcgt gagcctgacg agcattcaaa tcgcctatgg
     1441 ttgcatttaa atcgttttga gaagtgacaa tcagaaaaga ttgaccttgt gctttaaacg
     1501 aaagggtttc ggcaatggtt tgcatgctta acatcaatcg tacattttct gcaatgaact
     1561 gacccacctc atccacgcaa aaaacaagcc ggaagccagg ttgctttgat ttgatatact
     1621 tcgtaaccaa ttcaccaaag ccctctatat cgagcttgta gttgttaatc aggttatcaa
     1681 ggaagcgatt ggcttcatcg gtactgattg attttatctc tgcgtaaatt tctgccagtt
     1741 catgttgatt cagaaaaatg gcttcacggc ctttttccca actcttgttg aaacgtttct
     1801 gatattctgt tttgaaaaaa tcatatttac caatgctctc taaatgcctt tcgacttcag
     1861 ctatttccgg attcaaagga tcatagccta aaagttcatt gaacactttg aggaaaatgc
     1921 tcaacacggg atcaatggaa gagcccatgc ttgtgatgcc atcagatttg gctgcaatat
     1981 tgaacaggat tgattcagtt ggtatcgtag caactttctt gatattcctc tcaagctcaa
     2041 aatcatcaat tgccttttta gcaaatacgt cagcacattt gactccctta atctctttgt
     2101 tctctagcac aagggatagc attttaagca aatgcgactt accagaaccg aagtagccgg
     2161 aaatccagat gctgctgttg aagttgcttt ttgtcaattc ctcaaataag ccaggaagca
     2221 ttttgttcct gttggaaatg cctaacaatt cgttggtcag gacatattct tccaattcct
     2281 gcaaaaggtg cctgtcatcg ttggccttga tcactccctc aatagggcgg tcaatgtcct
     2341 tgagatataa ttctctttgt ttcatatcag attttataat cattgagatt gtgagcgcgg
     2401 taataattct cgtctttgat taattcaaac agggtaagag ttttgttgtt gtaagaaccc
     2461 gggaaaaaca tgactaacgg aatatttcca accagcgttt gtgtgttatt taaaatgcta
     2521 tgtgatcgaa taaatgggaa tacttcgcca actccggtca agaaaacgat cttagcatta
     2581 ctttcggcat ggcgtttatg gatttcagga ataattactg tttcgacatt cagattgctt
     2641 acaagaaccc tgtttaatcg aagtttgggc atattaacct cctgctcaac gatttgatcg
     2701 aatatgtttg ccctttttaa aatatcaaga cacaatttat aaagattgat ttctaaaatt
     2761 tcaatgccgt cattctgcaa gcgtttaacc aacgattgaa tgtttgcatt cacaacttgt
     2821 tgttggctaa ccggaaatga gtgaatgaag aatggtaact cgccnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnccgttt tttgcctggg tgattagacc gacctgttcc
     3001 aataaggtat aaacccgctg ctttactttc tttgctgttg attcagtaat tgcgttaagt
     3061 tcattatgag aaaggatttt ggtattaaaa aatttattgt agtctgtttc agttaatagg
     3121 ttatcaaaga tcaaatattt aataagaaga acctcagtga taaagtcttt aataaaagga
     3181 tatgttttca ccaaggataa caaaataatt gactttgcct catcacggct cccttgaacc
     3241 aataaattta attgatctga agtaagcaag gataatcgct ttttaatttc tctaaattcc
     3301 ctggcaaatg ttgcttgctt aattttgttc aatgaatttt tatccaataa tgatttccgg
     3361 actgcattcc aatctttcaa cctattgtat tcttcagcaa tgactgaagt ctctggtatt
     3421 aatgcagagg ctccggtgaa agaaaatgtg tattttttag ttgtcatcat tatatatttc
     3481 tgtaatagac cattcaattg cctgaggtcg ctgaattggt ctaaaaactg aattcaatgg
     3541 atttgaaaga gtcaggcggt gtaaattcaa atgctctata ctcttgtttt gtgtgatccg
     3601 tccaatgttt cggatgagga aagaacataa taatcagcct gttcttaaaa ttcttcaaca
     3661 ttggctttaa tggattaagg taattcatca agatatttgg tcccattggc agaatcataa
     3721 tccttgtgtc ggtatgtgct agaaggagtt tgttgatatc aatgactgat tcacgattat
     3781 tctcagcaaa ttccgattca atacaaagac atgccttgct gatgtaaggg atgttatgtt
     3841 tatgcgcaga taccaggtat tccttcttcc cgattaggat gtcaaaaaga tattcattgc
     3901 gacgaaattc catattcttt ttccctttag agaaaacgac aaatcctgat ttctcaggat
     3961 acttcttttc aaatgattga tgaatttcgt ttataaacaa agagcttcgc tgcttgttat
     4021 ccattctttc gacaccatta acctttaaca gtgtctgatg gaaagcgtca actatgcagg
     4081 atagatcgga atacataacc acaaggctgg ttgttaagat tcaattatgt aaaataatca
     4141 tgggtaatgg tttgaattta ttccctttcg aaattatagg tttttttaat actaatcgat
     4201 gggaatacag gcaagtctgg tatttaaaat cgttctaata tgcgcaccat gctcaatgtg
     4261 tccagttcac aatatttcaa gagattctcc ctcttggtgg ctatggactc tggatctgta
     4321 tcattatata gactttcata ggtcagacct gcagatcctc cttcctgtat ttcaaggttt
     4381 gaataggaaa ggtcgggcac cagcgctggc agaaccgctt tgagcgaata agaaccattc
     4441 atatcgggcg tgtaaaggta ctttttcctg aagggaacca ttaaatcgac cacccggtca
     4501 aacaaaggat caatcatgga ggaatactcc ggaaaatcac gggcgatctc ctttaacctt
     4561 gtgttttcaa aggtctgatt ccagacaatg atacttccgc tacttcctaa atgtgacaat
     4621 agggattgaa taaattcagg tcggggatca gttggtggag tgccaagaaa tgaaaattgc
     4681 gtcagggggc cacctttcga ttgctgcaga tgcaacgaat actggaacgg gatctgctgg
     4741 tatggcctac tttcgtcaaa ttggggaaca gcaaattgga tggtttcaaa gtccatgaaa
     4801 taaagagggt attcaagctc atctaaaaat tggtccagtt catcctggtt gcgggttgct
     4861 ggccggcctt cactcctatc ttcctcaaca ggaaagcttt gctgacagaa ttcgagaaaa
     4921 tcacaagtga atggattatt gcactgagat cccatggata tttcgggcat aaatcctgct
     4981 ttcagcatct cctgtaagga aagcagatca accgggatat tattctgcat tggcaataat
     5041 ctctcagtca ttggttcaat agcaaacaat ttccgaatat caaggtcccc atgccttaca
     5101 tattggttat ttacatgcac caaggatacc cgttttagcg gtagtcccga acgggttatt
     5161 acaaaatact ggaaggccac gtcgttcacc tgataatctt tgacctgtgt agaagctttc
     5221 acttcataag cagcccatcc atcaggctct ttcaccagga tatccatgta acaaagcgtc
     5281 tctccatcgc tgaaggcagc ttcgtagata acagtctgcc cattgttaat cagctccgct
     5341 gtataggtaa ttgcctcatg caccaaggaa tgatcgccac gactggcatc gatgccaccg
     5401 gggaagagtt gttgcgccag ttcgccaaca ttatggccga tgtcgaagat ctgttgctgt
     5461 gattcgctgg tttcatctcg ttcttctggc tgattcaaat gcaaccatag tgacttgtgg
     5521 cattgcatgc cgcggataaa tgaggatttt gagatcattt tactttccat atttttctat
     5581 ttttcaatca tgttccgctc attcatgaac tccaggatca tctctttttc aggcggggcc
     5641 atcgtatcat tatcaaatgc taccctattt atatcctcga aggttatcca tcctatattt
     5701 ctcgcgagac gctgccagtg ttcagatgaa atttcgttcc tatgcggaag agcacttttg
     5761 aggtttaatg ggtttttata ttcatccatt ttgtaacagt acaaacgtga ccgctgtggt
     5821 ttaaatatgt ccaaggatgt cttccgattc cccccataag attctgtacg aaaaatcttt
     5881 ggagtgagta atagaaagaa aatatcgtct ataattccct tgtttttatt gacatctttg
     5941 ctcttttgct gaacttctat tgccgaatcg atgttccgga ttatttgatc acgaacaggg
     6001 ttatatttta
//
LOCUS       DAOPYB010000184         5987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_16428_length_5987_cov_9.000169, whole genome shotgun sequence.
ACCESSION   DAOPYB010000184 DAOPYB010000000
VERSION     DAOPYB010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16428_length_5987_cov_9.000169"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      43..242
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            395..1165
                     /locus_tag="PKH58_11280"
     CDS             395..1165
                     /locus_tag="PKH58_11280"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HNX89658.1"
                     /translation="MKKLNYFLLAVLTAFVFVSCEKEENDTVMVNFEDVTLTDGIYNG
                     SDLTGTPQKETSWGTEITNYYKNIQTGPVKLLNIYTAEYKAWKGFAVSSKKDVTTNGY
                     ENQYSAITGSGAGGSAQYGVVFENGKIIFPDLAYNNLKSLMITNSTWAYKAITEGSGL
                     ARKFGAGDYFKVIFEGFKGNAVVATQEFYLADYRDGKSVVVKDWTKFILDGFDNIDYL
                     KISFDSSDKNEYGILTPTYVCIDNVEYQTVSVVCDCAK"
     gene            1452..1525
                     /locus_tag="PKH58_11285"
     tRNA            1452..1525
                     /locus_tag="PKH58_11285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1486..1488,aa:Thr,seq:tgt)
     gene            complement(1593..3305)
                     /locus_tag="PKH58_11290"
     CDS             complement(1593..3305)
                     /locus_tag="PKH58_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX89659.1"
                     /translation="MVKKIEPSPLTLQRIINKSVQKYAGLPALSFVDGTPMSYSELGL
                     KINETASLLRSLGLRKGDKVALLGHNMPNWVVAYFSVVSQGMVIVPILPDFSEEEIRN
                     VVEHSESKVLFVSERLTSRIENMEIPGLKARILLNDLSLAFGEQQIVSEKVSSDVVEE
                     VKEDDLAAIIYTSGTTGRSKGVMLTHKNIAFIAMQSYTFQSISSADVFLSLLPLSHTY
                     ENSIGLIYPIIYGASIYYLEKPPTASALLPALAKIRPTMMLSVPLIMEKLYQNQILKK
                     FSENKFKETIYNTPVFRKLIHRIAGKKLYQTFGGRLVFFGIGGAKLDSTVERFLREAN
                     FPYAIGYGLTESAPLLAGAGTKQTKLGSTGFAVQGVDLKINKPDKKGMGEIWAKGPNV
                     MSGYFKNPEATKEILTPDGWFRTGDYGYLDAFKRLYIKGRMKNTIISANGENIYPEDI
                     ESVINNNQYVLESLVTEEDGFLVAKILLDLNELEKNIEHIKAVIEDKKSKGLHTIQEL
                     DDKYKEWSQHILKKEINNKLNRVSQIKRVELIDKPLEKTATHKIKRFLYTKPTANKDD
                     MDIL"
     gene            complement(3613..4230)
                     /locus_tag="PKH58_11295"
     CDS             complement(3613..4230)
                     /locus_tag="PKH58_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89660.1"
                     /translation="MKKFIFSILLCLFVVSLQAQVKGKFRVGGNLGITMPTGGFGGAI
                     DVLDVRYNILDNLNAGVKLGGAFMLRDVAEISETKTEATMHLNSNIMLVGDYYFNKGT
                     SSFAPFIGGGIGSFSIYDIYMQAESGAEYNYDLDQIPSSERTYGGALRAGFEIGKFRM
                     ALEYYIIPETTKYNVDNITQPAGTSANNYVSLNLGFYFGGGKWKK"
     gene            complement(4442..5131)
                     /locus_tag="PKH58_11300"
     CDS             complement(4442..5131)
                     /locus_tag="PKH58_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89661.1"
                     /translation="MKNSYLIITAFFTSIIMSGCIVYQTQMTDVPLISEKNDLRIDAG
                     VSLIPAVQSTVSYGLTDKIAIQGYGNVGADHNYYFQTATGLYKNKGNDRVMEVYGGFG
                     YGYGDAYKDANPGNLLGNYQLYFGQFNYGKIASKSSGFEIGLGIKTGYLHSSLTDKNY
                     YDWGNTTGPYDIYKDESILFEPVGFMRFGSGRLKFSFKLGSTLIYKFTNTDKNLPYGR
                     LNIGLGLNYRL"
     gene            complement(5298..5819)
                     /locus_tag="PKH58_11305"
     CDS             complement(5298..5819)
                     /locus_tag="PKH58_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX89662.1"
                     /translation="MIETERLIIKPLTYGQLIKYIQCDNSLEKELNLNETSRTISPEL
                     KEALEQTILPNVADKTKNYLYSTLWTAISKTENKMVGDLCIIGEPNAEGEIEIGYGTY
                     PEFRNKGYMTEIVRGIIEWVKSQPNVKSIIASTDITNIASYKVLVKNRFVKTGETEML
                     LNWKLQMNNEINV"
BASE COUNT         1760 a         1197 c         1124 g         1906 t
ORIGIN      
        1 tattgttgat aaataaaatt tggtttattg aatttattgc ctacttttgc agaacgaatt
       61 ggtgccggta atttattcaa attttccggt aaaagggaat ccggtcagaa tccggaacag
      121 tacccgctgc tgtgaactcc attcaaacgt gccgaacaca cttgtgccac tgatacaaaa
      181 tcgggaaggc gttcggttaa cgggagtaag tcagaaaacc tgccattgaa taaaaaatgt
      241 ttttgccttc gggagttaac ggcaaaaaaa gaataagcac ttcatttacc gaagtaaaca
      301 aattctttat agtacatcta aattgtcaac tcctgaaata tttatctatt ttattgaggt
      361 gaattatatt tattaaccaa aaatatttca gaaaatgaaa aaattaaact acttcctgct
      421 tgctgtatta acagcttttg tatttgtttc gtgcgaaaaa gaagaaaatg acacggtcat
      481 ggtcaacttt gaggatgtaa cgctaaccga tggaatttac aacggctcgg acctcaccgg
      541 tacaccgcaa aaggaaactt cctggggaac tgaaattaca aactattaca aaaacattca
      601 gaccggtccg gttaagctcc tcaacatcta caccgccgaa tacaaagcat ggaagggatt
      661 tgccgtatca tctaaaaagg atgttaccac caacggttac gaaaatcagt acagcgccat
      721 aaccggaagc ggggcaggag ggtcagctca atatggtgtg gtgttcgaaa acggcaaaat
      781 catttttccg gacctggctt acaacaacct gaaaagtctc atgattacca acagcacctg
      841 ggcatataaa gcaattacgg aaggttcagg gttagcacgc aagtttggtg ccggtgatta
      901 ttttaaggta attttcgaag gatttaaagg aaacgcggtt gttgcaactc aggaatttta
      961 cctggctgat taccgcgatg gtaagtcagt agtggttaaa gactggacaa aattcattct
     1021 ggatggattt gacaatatcg attacctgaa gataagtttc gactcgtcgg ataagaacga
     1081 atacggcatt ctgacaccaa cctatgtgtg tatcgacaat gttgaatacc aaacggttag
     1141 cgttgtctgc gattgcgcaa aataacattc tgtaagttca ggctttcctg tctgccataa
     1201 tagtggtcgg aacttacact aagccgaatg agcaatcatt cggttttttt ttgtgctata
     1261 tatgtctgtt agcaagcaga ataaacatta gtgtaaaaaa attgacggtc aaaattttgt
     1321 cagttcgggc aaatgtagta cttttgtgcc gggtaagtcc tgcacgacca gctccctttg
     1381 aactccccca gggcttgacc gcagcaaggg taaaaggtcg tagcggtgcg atgcaggggg
     1441 cttacccacc tgcctcttta gctcagctgg ccagagcacg tgatttgtaa tctcggggtc
     1501 gttggttcga atccgacaag aggctcaaaa taagaaaagc cattctcaat gcgagagtgg
     1561 cttttttatt tgcgcgtgaa ataagtttta tattacagaa tatccatgtc gtctttattc
     1621 gctgttggtt tggtgtataa aaaccgtttt attttatgtg tggcagtttt ctccagcggt
     1681 ttatctataa gttctacccg cttaatctgt gaaacacggt ttaacttatt gtttatttct
     1741 tttttcagga tatgttgcga ccactctttg tatttgtcgt ccaactcttg aatagtatgt
     1801 aatcctttcg acttcttgtc ttcaattacc gctttgatat gttctatgtt tttttcgagt
     1861 tcattaagat caagaagaat ttttgctacc agaaatccat cttcttccgt tacaagtgat
     1921 tccaaaacat attgattatt attgataaca gattcgatat cttctggata aatattttca
     1981 ccattggcac ttataattgt atttttcatt ctacctttga tgtacaagcg tttaaatgca
     2041 tccagatagc cataatcacc tgttctgaac caaccgtctg gagtcaaaat ttctttggtc
     2101 gcttcaggat ttttaaaata tccagacatt acattgggcc cttttgccca tatttctccc
     2161 attccctttt tatcgggttt atttattttc aaatctactc cctgaacagc aaagccggtt
     2221 gaacccaatt tggtttgctt tgttccagct ccggccagca gtggagctga ttcggtgaga
     2281 ccgtaaccga tagcataagg gaaatttgct tctcttaaaa agcgctctac tgtgctatcc
     2341 aacttggcgc cgccaatacc aaaaaacacc aatctacctc caaaagtctg atacagtttt
     2401 ttccccgcta tgcggtgaat aagtttacga aagacaggag tattataaat cgtttcttta
     2461 aatttattct ccgaaaattt cttaagaatc tgattttgat aaagtttttc catgatcaac
     2521 ggcaccgaaa gcatcattgt cggtcttatt ttggccaaag caggcagcaa tgccgaagca
     2581 gtaggcggtt tttcgagata ataaatggat gcaccataaa tgatcgggta aatcagaccg
     2641 atactatttt cgtaagtgtg tgacaacgga agcagggaga gaaaaacgtc tgcactggaa
     2701 atactttgaa aggtatagga ctgcatggct ataaaggcaa tgttcttatg agtcagcatc
     2761 acacccttag agcgccctgt ggtgcccgaa gtgtaaatga tggcagccag atcatcttct
     2821 tttacttcct caacaacatc tgatgaaacc ttttccgaca cgatttgctg ttcaccgaaa
     2881 gcaagcgaca aatcattgag caatatccgt gctttcaatc caggaatttc catgttttca
     2941 atccgcgatg taagacgttc gcttacaaac aagacttttg attcggaatg ttcgaccaca
     3001 ttacggattt cttcctccga gaaatcgggg agaatgggca caataaccat accttgtgac
     3061 acaaccgaga aataggcgac aacccagtta ggcatgttgt ggcccagcaa agctacttta
     3121 tcacctttac gcaaacccag cgaacgcaac agcgatgctg tctcgttgat tttcagacct
     3181 aattcggaat aactcatcgg tgtcccatcc acaaacgaca aagcaggtaa accggcatat
     3241 ttttgtactg atttattgat gattctctga agggtgaggg gagatggttc tatttttttc
     3301 accatgtaat ttattgataa attcataaat aaagacacaa agataatgta ttggaattaa
     3361 tatctgcaca gacattgcgt aaacgtgcaa attattaaca tcaatatgaa tatagtacac
     3421 ccatgcaata cctcataaat tttagtatag cacagatatt tcaaaaaaat aaaccgccct
     3481 gagaaaaaac tacaggcggt ttattcaaat atgttttgac tatattaatc aggaaaattg
     3541 catttgactt aaatcattga cgaattcatg gagtgatttt tcaattgaat taagctggac
     3601 aattacaggt aattattttt tccattttcc gccaccaaaa tagaagccca gattaagtga
     3661 cacataatta ttggcagaag taccggcagg ttgtgtaata ttatccacat tatattttgt
     3721 tgtttcagga atgatataat attccaacgc catacggaat ttacctattt caaaaccggc
     3781 acgcaatgca ccgccataag ttctttcaga gctgggaatt tgatccagat cgtagttata
     3841 ttccgcaccg gattctgcct gcatataaat atcataaata ctaaatgaac ctataccacc
     3901 accaatgaat ggtgcaaaac tacttgttcc tttgttgaaa taataatctc caacaagcat
     3961 aatattggaa tttaagtgca tcgtggcttc agttttggtt tcacttattt cagctacatc
     4021 tctgagcatg aaagcccctc caagttttac tccggcattc aggttgtcaa ggatattata
     4081 acgaacatcc agaacatcaa ttgctccacc gaaaccgccg gtaggcattg ttataccgag
     4141 attacctcct acacgaaatt ttccttttac ctgtgcctgt aacgaaacaa cgaataaaca
     4201 caataagata ctgaaaataa atttcttcat atttttaagt tttaaaagta aataatggta
     4261 aagatatata ttgttgttaa ataaacttca ataaaacgaa aaaataattt cagatatgct
     4321 tcaaatgatg ccgttcagtg caaataaggg tgggggattg tcacgattac aaatccgtgt
     4381 tcaattaatt gtaatgtctt tttttgggaa cgctgatgac gctgattaac atggatttta
     4441 tctataatct gtaatttagt ccaagtccga tgttgagccg gccgtatggt aaatttttat
     4501 ctgtatttgt aaatttgtaa atcagggtgc tgcctagctt aaaactgaat ttcaatctgc
     4561 cacttccaaa cctcataaat ccaacaggtt caaagagaat actttcatcc ttatagatgt
     4621 cataaggtcc tgtcgtgtta ccccagtcgt aatagttttt gtctgtcagg ctggagtgca
     4681 ggtaaccagt ctttattccc agcccgatct caaatccgga cgatttactg gcgatcttcc
     4741 cgtaattgaa ttgcccgaaa taaagctgat aattgcccag caggtttccc gggttggcat
     4801 ccttgtacgc gtcgccataa ccatatccaa atcctccgta cacttccata acccggtcat
     4861 tgcctttgtt tttatacaaa cccgttgctg tctgaaaata gtagttatga tctgctccaa
     4921 cgtttccgta tccctgaatg gcaattttat ctgtcaaacc ataggagaca gtcgattgaa
     4981 ctgcggggat caacgaaact ccggcgtcaa tccgtaaatc attcttctca ctgatcaatg
     5041 gaacatctgt catttgtgtc tgatagacaa tgcaacccga catgatgatt gaagtaaaaa
     5101 atgcggttat aattaaatag ctgtttttca tgtttacagt ttataagatt aatatagtta
     5161 taacgactgg ttagtaaggg attgtggact aattatttat ccgccgtaac taagtttgta
     5221 cggggtttga ataccagatt taccactgaa acccttattg catccagcct ttattggctg
     5281 ttcgttgtta tttttcgtca aacattaatt tcattattca tttgcagttt ccaattcaga
     5341 agcatttcag tttctccggt ctttacaaac ctgtttttta ccaacacttt atacgatgca
     5401 atatttgtta tgtctgtcga tgcaattatt gattttacgt ttggttgtga tttgacccac
     5461 tcgataatac ctcttacaat ttccgtcata tatcctttat tccggaattc cggataagtt
     5521 ccataaccaa tttcaatttc tccttctgca tttggttcgc caattataca gaggtctcca
     5581 accatcttat tctctgtttt tgaaattgct gtccaaagcg tcgaatacaa atagtttttt
     5641 gttttgtccg ccacgtttgg aagaattgtt tgttccaacg cttctttaag ttcaggagaa
     5701 attgttctcg aagtttcgtt taagttcagt tctttctcca gtgaattgtc gcattgtata
     5761 tatttaatta actgcccgta agtcagtggc tttataatta gacgttctgt ttctatcatt
     5821 tttctatctg tttatgatgt tgtgaagttt cgtacaacga ctgcctatgt tttgccgctt
     5881 tgcgaaggca gatttttttc agcacaaagc ctgaatatag ttattgctgc cgcacgattg
     5941 tacctaaaat ccgcaagtgt attttctcat ttagcgttag gcgtttt
//
LOCUS       DAOPYB010000185         5986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_16435_length_5986_cov_9.397741, whole genome shotgun sequence.
ACCESSION   DAOPYB010000185 DAOPYB010000000
VERSION     DAOPYB010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16435_length_5986_cov_9.397741"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKH58_11310"
     CDS             complement(<1..602)
                     /locus_tag="PKH58_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNX89663.1"
                     /translation="MKKALIIGATGMVGAELTRQLLEDKNYGEIVSFVRRASGVSHPK
                     LTEHIVDFDKPESWKGLVTGDVLFSAMGTTIANAKTKDNQYKVDYTYQYETARMASEN
                     GVPVYVLVSSAGANSHAVAFYMNMKGELEDAVRKLPFQYLHIFRPGQLDGDRKENRAG
                     ERIALKLMYVINKAGIARRYRPIHATQLAKAMIIAAGTTSS"
     gene            complement(612..1835)
                     /gene="serB"
                     /locus_tag="PKH58_11315"
     CDS             complement(612..1835)
                     /gene="serB"
                     /locus_tag="PKH58_11315"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNX89664.1"
                     /translation="MNSEIILITISGEDKPGVTNAITTILGKYNATILDIGQADIHHN
                     LSLGILFKTSDDSNSGNILKEVLFKCNELNMHVRFKPVSEEKYENWVNRQGKSRYIVT
                     ILGKVIGAQQLAMVTRELAQQELNIDSIKRLTGRPSFHKEGENLRSCIELSVRGELKD
                     KNLLSEQFMILTSGEGIDISFQKDNIYRRNRRLICFDMDSTLIKTEVIDELADRAGVG
                     EQVRAITESAMRGEIDFTESFAQRVALLKGLDVSVMEEIARNLPIMEGADRLMSVLKM
                     CGYKIAILSGGFTYFGNYLKKRYDMDYVYANELEIADGKLTGRYVGDVVDGKRKAELL
                     RLIAQVEKIQLEQVIAVGDGANDLPMLNLAGLGIAFHAKPKVKESASQSISTIGLDGI
                     LYFLGFRDIHIDQEI"
     gene            complement(1868..3259)
                     /locus_tag="PKH58_11320"
     CDS             complement(1868..3259)
                     /locus_tag="PKH58_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNX89665.1"
                     /translation="MKIRFWIAIFVAACLLIGCKFITNKTRQSAEKKEISNSQNYFPK
                     DTLKVVTQYGATSYFNYRDEILGYDYELAQDLANHLHLKLKVEVAKNDDEMTNYLLLG
                     KADLAIYTTFETKQLKKVFNFVFPHRESYLVLVQMMGRNAVSDPTELVGKEVWVKNKS
                     FDHQRLLTLNDEIGGGIIVKLANDTLNTDDLMLQVASGKIKYTIAYRHKALLQKIFNR
                     QLDCRIPIGFNQRNGWLTRKGDRRLSDSIQAWSKLETTMHLRDKLYGKYWEKNPYFAF
                     KKIPIPRGAISPYDAIFKRHSKRIGWDWQLLAALAFVESGYDSTVVSWAGARGIMQLM
                     PQTALNFGVSRYEIENPEKNIAAGVEYIKSLDMIYRKISDKEERIKFILASYNCGPAH
                     ILDAMALAEKYGKNPHIWFNNVEYYLVKKSEPEFYNDPVVKYGYFRAKEPIRYVPNVL
                     DTYNKYMGIRTKS"
     gene            complement(3268..3993)
                     /locus_tag="PKH58_11325"
     CDS             complement(3268..3993)
                     /locus_tag="PKH58_11325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX89666.1"
                     /translation="MNPLLQKLMFIDIETVPSTEKYTELNGEMQHLWDEKFNLLRKRM
                     PEKYPEDSTPAFGYETSAGIYAEFGRIICISVGFIFIKNEEMHFRTKSFADEDEARVL
                     RDFSALIEKYPDYNFCGHNIKEFDIPYICRRMLINNLPLPIQLQINNRKPWEVNLVDT
                     LDYWKFGDYKNFTSLKLLTAVFGIPSPKDDIDGSLVAEVFYGEKNLPRIATYCQKDVL
                     ATAQIWLRMNGNPIIKSENIQHT"
     gene            complement(4083..4700)
                     /gene="udk"
                     /locus_tag="PKH58_11330"
     CDS             complement(4083..4700)
                     /gene="udk"
                     /locus_tag="PKH58_11330"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNX89667.1"
                     /translation="MLIIGIAGGTGSGKTTVVRKILQRLPEGEVVILPQDSYYRDSSH
                     LPLEERLEINFDHPDSIEFDLLTRHLKELKKGHSVEQPIYSYLTCTRAQETIPVFPRD
                     VVIVEGILVLSNPELRKMMDLKVFVDADPDDRLIRVINRDIVERGRSVNKVMERYEMT
                     VKPMHLQFIEPTKRFADLIIPQGGNNHIAIDILTKYIENNLNNKL"
     gene            4992..>5986
                     /locus_tag="PKH58_11335"
     CDS             4992..>5986
                     /locus_tag="PKH58_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNX89668.1"
                     /translation="MGLLYSVITGSGSYVPPKIISNNDFISNEFYDTNGEKLTTPGAV
                     IVNKFVEITTISERRYADENQKTSDLALIASQKAIKEAGIDKEELDYIIFAHNFGETS
                     HDNIKADIVPSLAARLKQKLGIKNPATVAYDVLFGCPGWLQCVIQADYYIRSGDAQKV
                     LVVGAEVLSRISDPHDRDSMIYADGAGAVVLEGRESDTPVGILGHNARTDAYLYAQML
                     HMGYSYNAELASQKNLFIKMNGRRLYMYALDNVPKAIKAGLDKLGLHISQIKKVLIHQ
                     ANGKMDDAILKRLYELYDIDEAPENVMPMTISKLGNSSVATLPTLYDYIINNQLEG"
BASE COUNT         1530 a         1288 c         1313 g         1855 t
ORIGIN      
        1 gatgaagttg ttcccgcggc tatgatcatg gctttggcaa gttgtgttgc atgaatgggg
       61 cggtatcggc gggcaatccc cgctttgttg ataacataca tcagtttcag agcgatgcgc
      121 tccccggcac ggttttcttt ccggtcgcca tccagctgcc ccggacggaa aatatgcagg
      181 tactgaaacg gtaatttcct tacggcatcc tcaagttcgc ctttcatgtt catgtagaaa
      241 gccaccgcgt ggctgttggc ccccgccgac gaaacgagca catacaccgg tacaccattt
      301 tccgaagcca ttctggccgt ttcatactga taggtgtaat caaccttgta ttgattgtcc
      361 ttggtcttgg cgttggctat ggtagttccc atcgcggaga acaatacatc gcccgtaacc
      421 agtcctttcc agctttcggg tttgtcaaag tcaacgatat gttctgttag tttcggatga
      481 cttactccgc ttgcacgccg tacaaagctt actatctccc cgtaattctt atcttctagc
      541 agttgtcggg taagttccgc gcccaccatg cctgtagcgc cgatgattaa tgcttttttc
      601 atctttatgg tttaaatttc ctgatcaata tgtatatccc ggaagccgag gaaatacaga
      661 atgccatcca gtccgatggt ggaaatggac tgactggcac tttccttcac cttgggtttg
      721 gcatgaaagg caatacccag tccggccaaa ttcaacatgg gcagatcgtt ggctccgtca
      781 cccactgcaa tcacttgttc cagttgaatt ttctctacct gtgcaatcag tcttagcagt
      841 tcggcttttc gcttgccgtc caccacatca cccacgtagc gtccggtgag ttttccgtcg
      901 gctatttcca gttcgttggc atagacgtaa tccatatcgt accttttttt caggtagttg
      961 ccgaaatagg taaatccgcc tgaaagaatg gcaattttat agccgcacat tttcagtacc
     1021 gacatcagcc ggtcggctcc ttccatgatg ggcaaatttc gcgctatttc ttccatcacg
     1081 cttacatcca gtcctttgag cagcgccacc cgttgtgcga aactttcggt aaaatcaatt
     1141 tcaccgcgca tggctgattc agtaatggca cgaacctgtt cacccacgcc ggcacggtcc
     1201 gccagttcgt cgatgacttc tgtttttatc agcgtcgaat ccatatcgaa gcagatcagt
     1261 cgtctattgc ggcggtagat attatctttc tgaaacgaaa tatcgatgcc ttcgccggag
     1321 gtgaggatca taaactgttc cgaaagcaga tttttatcct taagttcgcc tctcaccgat
     1381 aactcaatgc aggaacggag gttttctcct tctttatgaa acgacggacg accagtcaga
     1441 cgtttgatgg agtcgatgtt cagctcctgt tgtgccagtt cacgggtaac cattgccagt
     1501 tgttgcgcgc cgattacttt cccgaggatg gtcacgatat agcggctttt tccctgacgg
     1561 tttacccaat tttcgtattt ttcttcgctc accggtttga aacgaacatg catattcagt
     1621 tcgttacatt tgaaaagcac ctctttcagg atattgcccg aattactgtc gtcggaagtt
     1681 ttgaaaagaa tcccgagtga aaggttgtgg tgaatatcag cctgaccaat atccagaatg
     1741 gtcgcgttgt atttgcccag aatagtggtt atggcgttgg taactcccgg tttgtcttca
     1801 ccggatatgg taataagtat tatttcactg ttcatgataa tttttatatt ctgtttgtta
     1861 aaagtaatca gcttttagtc cggatgccca tgtatttatt ataggtatcg agtacatttg
     1921 ggacataccg aatgggctct tttgccctga aatagccgta tttcactacc gggtcgttgt
     1981 aaaattctgg ttcacttttc ttaaccaggt agtattccac gttgttgaac cagatatgag
     2041 gattttttcc gtatttttca gccagtgcca tggcatcgag gatgtgtgcc gggccacagt
     2101 tgtaactggc cagaatgaat ttgatgcgtt cttccttgtc gcttatcttg cggtaaatca
     2161 tatctaaact cttaatatat tccacacctg ccgcgatgtt tttttccggg ttctcaattt
     2221 cgtacctgct aactccgaaa ttcagcgctg tttgtggcat cagctgcatg ataccccgtg
     2281 cacctgccca cgaaacgacg gtgctgtcgt agcccgactc aacaaatgcc agtgcggcca
     2341 gcagctgcca gtcccaaccg atacgtttgg agtgacgttt gaatatggca tcgtaaggcg
     2401 atatggcacc gcgcggtatc gggatttttt tgaacgcgaa atagggattc ttttcccaat
     2461 atttcccata aagtttatcc ctgagatgca tggtagtctc aagtttgctc catgcctgaa
     2521 tggagtcgga caacctccgg tcaccttttc gtgtgagcca gccgttgcgt tggttgaagc
     2581 caatcggtat gcggcaatcg agttgacggt tgaaaatttt ctggagtaac gctttgtgac
     2641 ggtaagcaat agtatatttt attttaccgg atgcgacctg aagcatcagg tcgtcggtat
     2701 tcagcgtgtc atttgcgagc ttgacgatga tacctccgcc aatctcgtcg ttcaaagtca
     2761 gtaatctctg gtgatcaaag cttttatttt ttacccagac ttctttgcct accagctctg
     2821 tcggatcgga aacagcgttg cgccccatca tttgcaccag taccaggtaa gattctctgt
     2881 gcggaaagac aaaattaaac actttcttca gttgtttggt ttcgaaagtt gtgtaaatgg
     2941 ccagatcggc tttgcctaaa agcagataat tcgtcatttc atcatcgttt tttgcaacct
     3001 ccactttcag cttcaaatgc aggtgattag ccagatcttg tgccagctcg taatcgtatc
     3061 ccagaatttc atctctgtaa ttgaagtacg atgtggctcc gtattgcgtt acaactttca
     3121 gtgtgtcttt tggaaaataa ttttgtgaat tgctaatttc ctttttttca gcagactgtc
     3181 tggttttatt ggtgataaat ttacacccaa tcagcagaca cgcggcaaca aatattgcaa
     3241 tccaaaatct tattttcata ctctgtttta tgtgtgttgt atgttttcag attttattat
     3301 cggatttccg ttcatccgga gccagatttg agcagtagca agtacgtctt tctggcaata
     3361 ggtggctatg cggggcagat ttttctcccc gtaaaaaact tcagccacca ggctgccgtc
     3421 aatgtcgtcc ttgggtgaag ggatgccaaa gactgctgtg agtagtttca gcgaggtgaa
     3481 gttcttgtaa tcgccgaatt tccagtaatc gagcgtgtca acaaggttaa cttcccaggg
     3541 tttgcggtta ttgatttgca attggatggg tagcggcaga ttgttgatga gcatcctccg
     3601 gcagatgtag ggaatgtcga actctttaat attatgtccg caaaagttat aatcgggata
     3661 tttttcaatc aaagcgctaa aatcccgaag cacccgggct tcatcttcat cggcaaatga
     3721 ttttgtacgg aagtgcatct cttcattttt tataaagata aatcctaccg aaatgcagat
     3781 aatccgtccg aattcggcat aaatccccgc gcttgtttcg tacccgaacg ccggcgttga
     3841 atcttcggga tacttttcgg gcattcgctt acggagcagg ttgaattttt catcccacag
     3901 gtgttgcatt tccccgttca gttccgtgta cttttctgtt gacggtactg tttcgatatc
     3961 gataaacatc agtttctgta gcagaggatt cattctaagg tttttttagt gtttaactgg
     4021 cagaaaagat ttgcagaccg gactgtgccg taaagatgga tttcagtagc tgctgtttcg
     4081 ttttaaagtt tattattcag attattttca atgtatttag taaggatatc gatagctatg
     4141 tgattatttc caccctgtgg aattattaaa tcagcaaatc gttttgtcgg ttcaataaac
     4201 tgcagatgca tgggtttaac agtcatttcg tagcgttcca ttactttatt tacagacctg
     4261 ccacgttcta caatgtcgcg gttgatgacg cgtatcaggc ggtcatcggg gtcagcgtca
     4321 acaaatacct tcagatccat cattttacga agttcgggat tgctaagcac aagaatacct
     4381 tcgacaataa ccacatctcg tggaaaaacg ggaatggttt cctgtgcgcg cgtgcaggta
     4441 aggtaagaat agatgggttg ttctacggaa tgtccttttt ttagttcttt cagatgcctg
     4501 gtcagcaggt cgaattcaat ggaatcgggg tgatcgaaat tgatttccag tctttcctcg
     4561 agaggaaggt gactgctgtc cctgtaataa gagtcttgtg gcaatatcac cacttcccct
     4621 tcggggagac gttgtaagat tttgcgtact actgtggttt ttcctgaacc ggtgccgccg
     4681 gcaattccta ttataagcat gtttatggat ttatgatttt attttttcag agcactaaaa
     4741 tagtaaatat acattgatat ttttgcatat caacgaaaaa aacacaaaaa aatgttatgt
     4801 gaaacattca tatacaacat tataagttag ttaaagtatt ccttttataa agaaataaaa
     4861 tgtcaattga gagaaaataa atttaagttt tatcaaaaaa aatcaatctt ttaagtcctg
     4921 caggtgataa taggattagt ttcattatct ttgcaaaaat tttaatagtc ttaattgtaa
     4981 attagaagtt tatgggttta ttatacagcg taattaccgg ttcaggtagt tatgtacctc
     5041 ctaaaataat cagcaacaat gattttattt ccaatgaatt ttatgatacg aacggtgaaa
     5101 aactgacaac tccgggtgct gttattgtga ataaattcgt ggaaataaca accatttccg
     5161 aacgacgata tgctgatgaa aatcagaaaa cctctgacct ggcgctgatt gcttctcaaa
     5221 aagcgataaa agaagctggt attgacaaag aagagctgga ttacattatt ttcgctcaca
     5281 attttggtga aacttcgcat gataatatta aagctgatat agttccctca ttagcagccc
     5341 gtctgaaaca aaaactgggt ataaaaaatc cggctacagt agcatacgat gtgctttttg
     5401 gttgtccggg atggttacaa tgcgttattc aggctgatta ttatatccgt tcgggtgatg
     5461 ctcaaaaagt gcttgtagtt ggtgctgagg tactttcacg catttcagat ccgcatgatc
     5521 gcgacagtat gatttatgcc gacggagcag gagcagttgt gctcgaaggt cgcgaaagcg
     5581 atacaccggt gggaatactg gggcacaatg cacgtaccga tgcttatctt tatgcgcaga
     5641 tgttgcatat gggttattcg tacaatgccg agcttgccag ccaaaaaaac ctgtttatca
     5701 aaatgaacgg acgtcgtttg tatatgtatg cactcgacaa tgtaccaaaa gccattaaag
     5761 ccggactgga caaactaggt ctgcatatca gtcagattaa aaaagtgctt attcatcagg
     5821 ctaacggaaa aatggatgac gccattctga aacgattgta tgagttgtat gacattgacg
     5881 aagcgcccga gaacgtaatg ccgatgacaa tttctaaact aggaaattcg tctgtggcta
     5941 cattacccac attgtatgat tatattatca ataaccagct cgaagg
//
LOCUS       DAOPYB010000186         5982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_16452_length_5982_cov_10.074574, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000186 DAOPYB010000000
VERSION     DAOPYB010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16452_length_5982_cov_10.074574"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..989
                     /locus_tag="PKH58_11340"
     CDS             132..989
                     /locus_tag="PKH58_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX89669.1"
                     /translation="MLNSEFRFPLFDSILYGPMNPFSTSNSSEAFMLDEKPHLAVERE
                     DDRGVFVLSFLKPFNQKCSFYHQRLMRETVLYCNDLRAELSTLTDPDIRAYRRLTLLD
                     QHLKTALIKTGEVLRENHFTTSGLMSPSADSEPDAEKLSNCYIFQLLKVCLAKAYLEV
                     QHVLVPSGVKQLNENYLYTAFAGEAPPVTTWLRKKKIDELESIPHKPKPVEKKSRVVS
                     DEKSVGVSEKLLTLREIEQLGYGSGRTLRRWLDAGKLKGFKTGNIWKVEESELNRYKA
                     ELKTKTPDK"
     gene            986..3136
                     /locus_tag="PKH58_11345"
     CDS             986..3136
                     /locus_tag="PKH58_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89670.1"
                     /translation="MSKKPVELTLNALKIRFKEEKITVYPSNKSTADAVKFSQNEAAE
                     LKRVYKLTDEWEDVYARLVPETPGQLSVTKAVSPEFCRENETCWSLGFLKKYCTRRLT
                     LHFQSLGFPCRTNFVSDTEVWIKALSPYANCTGYRVITLRVQFDAGEKTPELLVIAGE
                     IRSVYNGAVTDPVFSEMDDKWIETVVYKTGIMKYRYLSDDARRNLSEVFPCLNPPLKQ
                     LLGIPYTAPDKGNRYVKFLHEAEQFKNEYLLNPDLSRVFEIASDWKKAMPQYFDTEEL
                     IKMSFGTGTHMQPKYGLPQFGPAELLDDPVVFFFIGHVDDKPLASTINDYFLGKFSTE
                     FKGINGYLHLKYVTEPKSSIWFTDKHNPLPEIKSALKAKLENQESDSRKRYVAIYLSP
                     HSKYGSSPVEHKIYYQVKEHLLSCGIVSQVVEAGKAWGKGRPLLPKTDSDKHDKAKLA
                     KGFHYSFANIAVAIYAKLGGIPWSFGKQASDELVIGVSAYTNRQLGKYLGSAFSFTNE
                     GRFQGFECFSNTQLPQLAGSIKLALMKSTQENPAIKRLIIHFYKKLSYDEIKPIQKAL
                     SELQLEIPVVVVTVNKTFSEDLVGFDLSVTHLMPASGHYLRIDKDRYLLYNNQLTKGV
                     TVIPSDREGYPFPLKITIQQYEPGSNEAKPLSEEDVVPVMQQLCRFSTLYWKSVSRQW
                     LPVTLRYPEMLAQIAPHFKYKDWGELGNDSLWFL"
     gene            3531..4709
                     /locus_tag="PKH58_11350"
     CDS             3531..4709
                     /locus_tag="PKH58_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX89671.1"
                     /translation="MRINREITNAMKELIVKYPVLALTGPRQSGKTTLLRSIFPEYTY
                     ISLENPDNRNFAETDPNGFLKLYSKHVIFDEVQRVPDLFSYIQSMVDESGLMGQFILS
                     GSQNFHLMRNITQSLAGRVALFKLFPFDFEELISADLINNDYLENIIKGFYPAIYDRS
                     IHPGTFYSNYMQTYIQRDVSELIAIKDLRLFQNFLGLCATRAGQLLNLNSLANECGIS
                     QPTAKAWLSALESSYIVFQLYPYHENFSKRIVKTPKLYFYDSGLLSYLLKIKNAESLS
                     THPFKGSLFENMMIAEYVKRMYHQNQVVDLWFWRDSAGHEVDLLINDGITLNLVEFKA
                     TQTVMTDLFKGLNFFEKESKKDRLTKTLVYAGDQKQSRSAGNVVPWLEFGASVLFNKN
                     "
     gene            5488..>5982
                     /locus_tag="PKH58_11355"
     CDS             5488..>5982
                     /locus_tag="PKH58_11355"
                     /inference="COORDINATES: protein motif:HMM:NF039392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6266 family protein"
                     /protein_id="HNX89672.1"
                     /translation="MGKLNQGILGGFSGAVGTVVGSTNRKGEDIIRAKSKKARPASSA
                     GQVKQQTKFALVTGFMQGVNPVLNAGLKNAARAENISPYNYASRHALMNAVAGTDENP
                     ELDYANVLLSDGALNRIPGATAVLTDNEVVFGWSDVPAGSKGEAADTVCLVVYNVTNR
                     ELSFS"
BASE COUNT         1866 a         1193 c         1375 g         1548 t
ORIGIN      
        1 aattctatca aaatgtcaat aactttttat ttttattcat tttgagttaa aaataaatct
       61 gtatatttgt ggtatgatta tagttacaat ctgatagtta gcaggttgga cttcaaaaaa
      121 aacgaatata catgctgaat tccgaattcc gttttcctct ttttgatagc atcctgtatg
      181 gtccgatgaa tcctttttca acgtcgaaca gcagcgaggc atttatgctg gacgaaaaac
      241 ctcacctggc ggtggagaga gaagacgacc ggggcgtttt cgtcctttcc ttcctgaagc
      301 cgtttaatca gaagtgttcg ttctatcacc agcggttgat gcgcgaaacg gtattgtact
      361 gcaatgatct gagggcggaa ttgagcacac tcaccgatcc cgatatcagg gcttaccggc
      421 ggctgacctt actcgaccaa catctgaaaa ccgcacttat aaaaaccgga gaagtgctgc
      481 gcgaaaacca ttttaccact tccggtctga tgtcaccatc agccgacagc gaacccgatg
      541 ccgaaaaatt gtccaactgc tatatctttc aattgctgaa agtttgtctg gccaaagctt
      601 acctggaggt tcagcacgtt ctggtgcctt cgggcgtaaa acagttgaat gaaaactatc
      661 tttacacggc atttgcaggt gaagcaccgc cggtaacaac ctggttaaga aaaaagaaaa
      721 tcgacgagct tgaaagtatc ccacacaagc cgaaaccggt ggaaaagaaa tcccgggttg
      781 tgtctgatga aaaatccgtg ggagtatcag agaaattact caccttgaga gaaattgaac
      841 aactgggata cggcagcggg cgaacattgc gcagatggtt agacgcagga aaattaaaag
      901 gatttaaaac tggaaatata tggaaagttg aagagagcga actaaaccgt tacaaagcag
      961 aattaaaaac caaaacccct gataaatgag taaaaagccg gtagaactta cgttaaatgc
     1021 actgaaaatc agatttaaag aagaaaaaat tactgtttac ccaagcaaca aatccacagc
     1081 cgatgctgtg aagttttcgc aaaatgaggc cgcagaactg aagcgggttt ataaactgac
     1141 cgatgaatgg gaagatgtat atgcccgttt ggttcccgaa acgcccggtc agctttcggt
     1201 caccaaagcg gtaagtcctg aattttgccg tgaaaatgaa acctgctggt ctctcgggtt
     1261 tctgaaaaaa tactgcaccc gccggctgac ccttcatttc caatcgctgg ggtttccctg
     1321 ccggacaaat tttgtgtcgg acaccgaagt gtggattaaa gcactgtcgc cttatgccaa
     1381 ttgtaccggt taccgggtga ttacgctgcg ggttcagttt gatgccggag aaaaaacgcc
     1441 ggaactactg gtgattgcgg gcgaaatccg ttcggtgtac aacggggcag taaccgatcc
     1501 ggtattttct gaaatggacg ataaatggat agaaacggtg gtgtataaaa ctggaataat
     1561 gaagtacagg tacctgagcg atgatgcacg ccgaaatcta tccgaagtat ttccctgcct
     1621 gaaccctccg ctgaagcagt tgctcggcat accctacacc gcaccagata agggaaaccg
     1681 gtatgtgaaa ttcctgcatg aagcggagca attcaaaaat gaatacctgt taaatccgga
     1741 tttatcccgc gtctttgaaa tagcttccga ctggaaaaag gcgatgccgc agtatttcga
     1801 caccgaagag ttgataaaaa tgagctttgg taccggtacg cacatgcagc caaaatacgg
     1861 tttgcctcag tttggacccg ccgaactgct ggatgatccg gtggtgtttt tctttatcgg
     1921 acacgtggat gacaagcccc tggcctcgac cataaacgac tatttcctgg gtaaattcag
     1981 tacggagttt aagggaatta acggatattt acacctgaaa tacgtaacag aacccaaatc
     2041 aagtatctgg tttaccgata aacacaaccc gctgcccgaa attaaaagtg ccttgaaagc
     2101 caaactggaa aatcaggaaa gcgatagccg gaaaaggtat gtggccattt atctctcccc
     2161 tcattccaaa tatggatcat cgcctgtcga gcataagatt tattatcagg taaaagaaca
     2221 tttactatcc tgcggcattg tgtcgcaggt ggtggaggca ggcaaagcct ggggaaaggg
     2281 gcgtccgctg ctgccaaaaa ccgacagtga caagcacgat aaggcaaaat tagcaaaagg
     2341 atttcattat agttttgcca atatcgccgt cgccatttac gccaagctgg gcggaatacc
     2401 gtggagtttc gggaaacagg caagcgatga actcgtaatc ggggtgagtg cctacaccaa
     2461 ccgtcagttg gggaaatacc tcgggagcgc attcagtttt accaacgaag gacgatttca
     2521 ggggttcgaa tgtttcagca acacccaatt gccccaactg gccgggtcga taaagctggc
     2581 actcatgaaa tcgacacaag aaaacccggc aataaaaaga ctgattattc acttttacaa
     2641 gaagctgagt tacgacgaaa tcaaacccat tcagaaagcc ctctcggaac tgcaactcga
     2701 gataccggta gtggttgtaa cggtgaataa aacattttcg gaagatttgg tcggtttcga
     2761 tctttcagtt acccacctga tgcccgcgag tggtcattac ctgcgcatag ataaggatag
     2821 atatctgctt tacaacaatc agctgactaa aggagtaacc gttataccat ccgaccgtga
     2881 gggatatccc tttccgttga aaattacaat tcagcaatac gaaccgggca gcaacgaggc
     2941 taaaccgttg agcgaggaag acgtagtgcc ggtcatgcag caactttgcc ggtttagcac
     3001 cctctactgg aaatcggtgt cacgccagtg gctgccggtt accctgcgct accccgaaat
     3061 gctggcccag atagcaccac actttaagta caaagactgg ggagaactcg ggaatgatag
     3121 tttgtggttt ttgtagggaa aaaagagcaa agagcaaaga gcaaagagca aagacagaag
     3181 acaagatgac aagatgacaa gatgacaaga tgacaaaaga caaaagagag gtaaatttcg
     3241 acacaaatca gtttggatta aatgcctgaa aaattcacga aatgacagtt tttaaaaaaa
     3301 tctaccgggt gtagcggttt ccaaccgcta aaaataaatc cgcggttaaa aaccgcggca
     3361 cccgaataaa tctgcggcag gaaacctacc aatgcgtagg caatgcgaat aaattaagta
     3421 atttcaaaat aagagctttg taatttcaaa attgaagtca tgtaatttca aagtagaagt
     3481 attgtaattt caaaattaat tgtatatttg catttgtaaa taaacatatt atgagaataa
     3541 atagagaaat tacaaatgcg atgaaagaac tcatcgtaaa atatccagtt cttgcactta
     3601 caggtccgcg tcaatcgggt aagacaacgc tactgagatc aatttttccg gaatacacct
     3661 acataagtct tgaaaatccc gacaacagaa actttgcaga aaccgatccc aatggatttt
     3721 tgaaactgta ctccaagcat gttatttttg atgaagtgca gcgggtacct gacctttttt
     3781 cgtacataca atctatggta gatgaaagtg gattgatggg acagtttatt ttatcgggtt
     3841 cacagaattt tcatctaatg cggaatatta cccaaagttt agctggaaga gtagccctat
     3901 ttaaactttt tcccttcgat tttgaggaat tgatttcagc tgatttgatc aataacgatt
     3961 atttggaaaa tataatcaag ggtttttatc cggccattta tgataggtct atacaccctg
     4021 gtacgtttta tagtaattat atgcaaactt atattcaacg tgatgtaagt gagttgattg
     4081 ctattaagga tttgcgattg tttcagaatt ttctgggatt atgtgccaca cgtgccggtc
     4141 agttgctgaa tttgaactct cttgccaacg aatgcggtat ttcacaaccg actgccaagg
     4201 cctggctttc cgctttagaa agtagttata tagtttttca actctatcct tatcacgaaa
     4261 attttagtaa gcgcattgtg aaaactccaa aattgtactt ttatgattca ggtttgctta
     4321 gttacctttt aaaaataaag aatgctgaat cattatcgac tcatccattt aaagggagtt
     4381 tgttcgaaaa catgatgata gctgaatatg tgaagagaat gtatcatcaa aatcaggtag
     4441 tggatctatg gttttggcgt gattcggcag gtcatgaggt agatttactc ataaatgatg
     4501 gaattacgtt gaatttagtt gaattcaaag caacccaaac tgttatgact gatttattca
     4561 agggattaaa cttttttgaa aaagaatcta aaaaagatcg tttaaccaaa acattagtat
     4621 atgcgggaga tcagaaacaa agcagatcag caggtaatgt agttccctgg ctcgaatttg
     4681 gtgcgtcagt tttgtttaat aagaattaga tttaagtcag ctctgacatt atactacctg
     4741 ccgtgtatta gaagtgtttt tagtgcgttt tttacgaaaa ggaggagtaa gcagatattc
     4801 agttagttat gctctacttt gtcaaaactt caattttaat aaagttttga taaagatgca
     4861 gtaagatcgt aaaatagcaa agaaaagtgt gaataattta ctttggaatt tacgcatttt
     4921 gcattgttag ggttttgcat tgctttattt cacgaatatt gggtaaaatc tgccggatgt
     4981 agtggtttcc aaccgctatc aattaatccg cggttgataa ccgcggcatc cgaaaaagtc
     5041 tgcggcatga aatttaccag tgcaacatag catttcaatt cctgtaactt ttacagcatt
     5101 agaattgtct gactaatggc acttcatttg gaaacgaggg gggaggtaag tagtttgaga
     5161 aatcagactg ctttttttat atccggtaac gaacgaagag aaccggacaa cggcggacat
     5221 cacggacatt tttatccgga aaaagttccg gcaaaaagtc tggcacggcc atttgcggac
     5281 aatgcggaca ttttctgttg gtggcaaact tgttgtaatt aatcctggcg tagggcgcta
     5341 caggtttaat gaaaacccgc catcaatctg atcaattgaa aacggaaagc cattaaagaa
     5401 cggttcgaac gcacgacgac tgatcatcag gatgcaggga ggaccagatt gcaaaaaaga
     5461 atatttaccc cataaaaaat taagaaagtg ggaaagttga accaaggtat tctgggagga
     5521 ttctcgggag ccgtaggaac cgtggtgggt agcaccaaca gaaaaggcga agatattatc
     5581 agagccaagt ctaaaaaagc acgtccggca agcagtgccg gacaagtaaa acaacaaacg
     5641 aagttcgcgc tcgtcaccgg cttcatgcag ggtgtaaatc ctgtgctgaa tgcaggactg
     5701 aagaacgccg caagagctga aaacatttcg ccttacaact acgccagccg tcatgcgctg
     5761 atgaatgccg tggcaggtac ggatgaaaat ccggagttgg attacgcgaa tgtattgctc
     5821 agcgatggtg ctctcaacag aataccgggt gcaacggccg tactcaccga caatgaggtg
     5881 gtgttcggct ggagcgatgt acccgctggt tcgaaaggcg aggcggccga taccgtttgt
     5941 ttggtggtgt ataacgtgac caaccgggaa ctgagtttct cg
//
LOCUS       DAOPYB010000187         5973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_16490_length_5973_cov_8.833897, whole genome shotgun sequence.
ACCESSION   DAOPYB010000187 DAOPYB010000000
VERSION     DAOPYB010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16490_length_5973_cov_8.833897"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..638)
                     /locus_tag="PKH58_11360"
     CDS             complement(45..638)
                     /locus_tag="PKH58_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89673.1"
                     /translation="MAFTPCTTAGLTVNLAKSCTEPRIKGYEQIGLIILKSDIDLALT
                     SVDTTNPRIIYNLALKATKKASVIYNSKKAPLPFNGTQTAYNREADAYDKTVQFYYEG
                     IGGAVSKNAIEPLKDGSYVVVLERKSKYGAGSFQVFGWQVGLSCGNDGGAQVQDEETG
                     YWLITMTCQEPYAEIEWLDSANTQETKIAFDGLAVGM"
     gene            complement(666..1052)
                     /locus_tag="PKH58_11365"
     CDS             complement(666..1052)
                     /locus_tag="PKH58_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89674.1"
                     /translation="MTANDYILSGYEISKQVTEEVITRAESDVMTAYILPIIGSDAKK
                     EDYRCEVMALAYCLLLRRNIVKTRFGSEVKVNQYGTVMQQENAMLNSQICGMCSIAIS
                     GLKGKTLSTENFNRVKDIIEFGYYKY"
     gene            complement(1049..1216)
                     /locus_tag="PKH58_11370"
     CDS             complement(1049..1216)
                     /locus_tag="PKH58_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89675.1"
                     /translation="MKITFKYNYTLLSNGEIRTFKAGESQDFPADSPLIETFKQQDGD
                     VLMIEKKSKKK"
     gene            complement(1223..2455)
                     /locus_tag="PKH58_11375"
     CDS             complement(1223..2455)
                     /locus_tag="PKH58_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89676.1"
                     /translation="MKKIYLQSTLTALKNNLAKNTVTPEGQALIDEITTILSDLANDP
                     DVEYNETDVMAKVTEAAKSAGASAAAEVASAFRGKEAKNKVDLKLARGIYEEALNNSQ
                     GKGRGAFANEISALCVKNGITGLPSILEIFPEIQTAWKNTSILSKLKKLGKYALTFGI
                     STQSDDDDDVRAGGHIIGNAKVNQALALTPKTINLGAIYKKIDVPKLTSYQTGNDTAL
                     FAWLVQELFDRLNDEVTRAILVGDGRAANSPRKISSFETIGIKTAADAYTVYKNISNA
                     VPTLADIRAMVDDMDDSKPISLYLHPTIKRSVQAYQYAAGGTISYVTDEVLAEQLGVS
                     EIIKYKKLAGAVPAVASTVPCVIAIQHDSYGYVGSDVFSANFEDWNYNTDNLITELFA
                     GGAIIKPLSTGLITITKP"
     gene            complement(2480..3067)
                     /locus_tag="PKH58_11380"
     CDS             complement(2480..3067)
                     /locus_tag="PKH58_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89677.1"
                     /translation="MEKQLQFDEKMIARNFFAINDFSKAENGNFKVSGLLSVFDREED
                     NMNGYVYHAGCYDDFCKNYFEKNMKNIPLDILHNTMDIYHLAGKVTEFSVTNSEARIV
                     AEVSRFTPLFENIVGLIEDEVLQGFSDMSFVNDGYFDGTKNLFHVKQCSIFSVTLTQN
                     PAVAKSQLKAMNATKFNFESIKTKTEEKKSTFFGL"
     gene            complement(3045..3953)
                     /locus_tag="PKH58_11385"
     CDS             complement(3045..3953)
                     /locus_tag="PKH58_11385"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNX89678.1"
                     /translation="MKLLFETDSKKRAVAQRGYNGVMGFRMTRGKYSDVLLMSIFKKI
                     FKRLNLIDYTVNSPDAITTGSVSYFLNNEMSAAFAELFQNGFVLFHKNSVGVFKYIRK
                     NKATQTPEGWYYENQPLDVFYSDTYEVFGMSDGIMLKDTLKAIDSALNANQTIIKRLG
                     VVVTGTPEQPSQNPQMVQMDGDEKDEMEKEIQNDYGMLEEQKQFLLFRRPMKLQRIAL
                     GGKDLMITETVETFTKILCDATNIPYDVMAMSGQSTFNNMEQAEKSMQDTAEEFVSKI
                     WTFLKVMNIDFSWKVNNSKYGKAITV"
     gene            complement(4189..5766)
                     /locus_tag="PKH58_11390"
     CDS             complement(4189..5766)
                     /locus_tag="PKH58_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89679.1"
                     /translation="MANPKNIIPPKKGEIRNPKGRGNSLNRKTLMRKWLTLTEEGKNP
                     ITKETEKLSQADLVMLAMINKARKGDVSAGKLLIEHGTDEERENTNDNNIQTAPTLVN
                     YMNSVADDWLQQRTFIETYKILEQIHDFCFDTDNKILLLSMPQGSGKSYIANKVTEWL
                     LGCWQLVARTQSNSIMRICNTDSNVTKFQAAISNEIMGDSWSAVFGEHKLSNNNKNGM
                     RFDGSWNDNAFFTSAKSSVMSRRADFLIFDDLYASMGEALSHTMTADYIMKFQTMWRG
                     RLKGSEIGKIIMVGTRYAKNDFYQQVINMYLENSVVISIPAIDDDGNSFCEETHPIDE
                     LLHDMETMNIDLFNAIYQQNPTAEGFINPFENWTPTTALLTDTQFDYTCTIADPSFGV
                     GGDYFMVGKFGYNRNGSFALLDLLSERVCSDEMYIDFIEKAGCKRNFIEGNGVGGMLI
                     KRVCNRTKAQLVPFTSIGNKLERIYMNANDIRKLTFNETIENIPFDQMINFGLSEHDD
                     FPDMLSHFFNNIKVYGK"
BASE COUNT         1703 a         1249 c          894 g         2127 t
ORIGIN      
        1 caatcaatta accacttttt tcaaaaaaga tttccaaatt ttaactacat gcctactgcg
       61 agtccgtcaa aggctatttt tgtttcttga gtattagctg aatctaacca ttcaatttct
      121 gcgtatggct cttgacaagt catcgttatt aaccaatagc ctgtttcctc gtcttgaacc
      181 tgtgcgcctc cgtcattacc acaacttaat cctacctgcc atccgaaaac ctgaaaagaa
      241 cctgctccgt atttagattt tctttcaaga acaaccacat aagaaccgtc tttcaaaggt
      301 tcaatggcgt tctttgaaac agctcctcca atgccttcat aataaaactg aacagtctta
      361 tcgtatgcat cagcttcacg attgtaagcc gtttgagtcc cattgaaagg aagaggtgct
      421 ttcttgctgt tataaataac ggatgccttt tttgtagctt ttaaagcgag gttataaatt
      481 attctcggat ttgtagtatc aactgatgtt aatgccaaat caatatcaga tttaagaatt
      541 ataagtccta tttgttcata gcctttaatt ctcggctcgg tgcaactttt tgccaaatta
      601 accgttagcc cggctgttgt acatggtgta aatgccatat ttttttctcc tattttttat
      661 gtaaattaat atttatagta accaaattca attatatctt ttacccgatt gaaattttct
      721 gttgaaagtg ttttgccttt taagcctgaa attgcaatgc tgcacatccc acaaatttga
      781 gaatttaaca tcgcattttc ttgctgcata acagtgccat attgattaac ttttacctcc
      841 gaaccaaaac gagtcttcac aatgtttcgt cttagcaata agcaataagc caaagccata
      901 acttcacatc tgtaatcctc tttttttgca tcacttccaa tgataggcaa aatataagca
      961 gtcatcacat cgctttcggc tcttgtaatt acttcctctg ttacctgttt tgaaatttcg
     1021 taaccggata aaatataatc gtttgctgtc attttttctt agatttcttt tcaatcatta
     1081 gaacatctcc atcctgttgt ttgaatgttt caattaacgg actgtcggct ggaaagtcct
     1141 gagactctcc tgccttaaat gtccgaattt ctccattcga taacaaggta tagttatatt
     1201 tgaatgtgat tttcatattt tattacggtt ttgtaattgt gatcaaacct gttgaaagag
     1261 gcttgataat tgcaccacct gcgaataatt cagtgataag attgtcagtg ttataattcc
     1321 aatcttcaaa attagcactg aaaacgtcag agcctacata gccataactg tcatgctgaa
     1381 tagcaatcac acaaggaact gtcgaagcca ccgccggaac agcacctgca agtttcttgt
     1441 attttatgat ttcagaaact cccaactgtt ctgctaaaac ttcatcggtt acatagctaa
     1501 tcgttccacc tgcagcatat tgatatgcct gaacactacg tttaattgta gggtgcaaat
     1561 ataatgatat tggcttacta tcatccatat catcaaccat tgcacgaata tcagccaatg
     1621 ttggtacagc gtttgaaata tttttataaa cagtataagc atctgcagct gttttaatac
     1681 cgattgtttc aaaacttgaa atttttcggg ggctgtttgc tgcacgccca tcgccaacca
     1741 aaatagcacg agttacttca tcattcaagc ggtcgaacaa ttcctgtacc aaccatgcaa
     1801 ataaagcggt gtcattgcca gtttgatagc ttgtcaattt aggaacatca atctttttgt
     1861 agattgcacc taaattaata gttttcggag tgagtgccaa tgcctgatta acctttgcat
     1921 ttccgataat atgaccacct gcacgaacat catcatcatc gtcagattga gttgatattc
     1981 caaatgttaa ggcgtattta cctaactttt taagtttgga taaaatactg gtatttttcc
     2041 atgctgtttg aatttcaggg aaaatttcca aaatagaagg aagtccggta ataccatttt
     2101 ttacgcaaag agctgaaatc tcatttgcaa aagctccacg acctttacct tgagaattat
     2161 tcaacgcttc ttcataaatt ccgcgagcta atttcaaatc aactttgttt ttagcttctt
     2221 ttcctctaaa tgcgcttgcg acttcggctg ctgctgatgc acctgctgat ttcgctgctt
     2281 cggtaacttt cgccataaca tcggtttcgt tatactccac atccggatca tttgccaaat
     2341 ctgataaaat cgttgtgatc tcatcaatga gtgcctgacc ttcgggagta accgtatttt
     2401 ttgctaaatt atttttgaga gctgttagcg tactctgtag atagattttt ttcatagttc
     2461 taaaaaattt gtttattgtt tacaatccaa aaaatgttga tttcttttct tctgtttttg
     2521 tttttatact ttcaaaatta aatttcgttg cattcatagc cttcagttga cttttagcaa
     2581 ctgccggatt ttgtgtaagc gtgactgaaa atatactgca ttgttttaca tgaaaaaggt
     2641 tttttgttcc atcaaaatat ccatcattta caaatgacat atctgaaaat ccctgcaaaa
     2701 cttcatcctc aatcaatcca actatatttt caaataacgg tgtgaaccga cttacttcgg
     2761 caactattcg agcttcgcta tttgtcacgc taaattctgt aacctttcct gctaaatggt
     2821 aaatatccat tgtattgtgt aaaatatcta atggaatatt cttcatattt ttttcaaaat
     2881 agtttttgca aaaatcatcg taacatccgg catgataaac ataaccattc atattatctt
     2941 cctctctgtc aaaaacggac aataatccgc ttaccttaaa attaccattc tcggcttttg
     3001 aaaaatcatt gatggcgaaa aaattacgtg ctatcatttt ttcatcaaac tgtaattgct
     3061 tttccatatt ttgaattatt tactttccaa ctaaaatcta tattcataac ttttaaaaac
     3121 gtccaaatct tagagacaaa ttcttccgct gtatcttgca tggatttctc ggcttgctcc
     3181 atattgttaa atgtactttg cccggacatc gccattacat cataaggaat attagtcgca
     3241 tcacaaagga tttttgtaaa tgtctctacc gtttctgtta tcatcaaatc ttttccaccc
     3301 agggcaatcc gttgtaattt catcggtctg cgaaaaagta agaattgttt ctgttcttcc
     3361 agcattccat aatcgttttg tatttctttt tccatctcat ccttttcgtc accatccatt
     3421 tgcaccattt gaggattttg gctcggttgc tccggtgtac cagttaccac gacgcccaat
     3481 ctttttatta tagtctgatt tgcattaagt gcactgtcaa tagctttcaa agtgtctttt
     3541 aacattatac catccgacat cccgaaaacc tcgtatgtgt ctgaataaaa aacatctaac
     3601 ggttgatttt cataatacca tccttcgggt gtttgagtgg ctttgttttt ccttatatat
     3661 ttgaaaacgc ctactgaatt tttatgaaac aatacaaaac cgttttgaaa taattcagca
     3721 aatgcagccg acatctcatt attcaaaaaa tatgaaacgc tccctgttgt aattgcatca
     3781 ggactgttaa cggtataatc aattaaattc aatcgtttga aaatcttttt gaaaatagac
     3841 atcaacaaga catcggagta ttttcctctc gtcatccgaa aacccatcac gccattatat
     3901 cctcgttgcg ctacggctct ttttttactg tctgtttcaa ataataattt catattgtta
     3961 aattttgaat gtcacaaata taaatgtttt attttgaata agcacttttt tgtgtacatc
     4021 tttttaaatt agtcaaaatc gcgttcatat ttgtaaataa tatatttcga tatttcacct
     4081 ccataaacct ttgaaacttt ctccgctgac atacgttttc ttaaatgaca tatgtcttgc
     4141 caatgctgat atacaaaaat ctttttcttt ccataattgt ctttgtgatt atttgccata
     4201 gacttttatg ttattgaaaa agtggcttaa catatcaggg aagtcgtcat gttccgaaag
     4261 tccaaaatta atcatctgat caaacggtat attctcaatt gtttcattga atgttagttt
     4321 ccttatatcg tttgcgttca tataaattct ctccaactta ttacctatcg atgtgaaagg
     4381 aaccaactgt gctttcgtcc tgttgcaaac tcttttgata agcattccgc caactccgtt
     4441 gccttcaata aaatttcgtt tgcatcctgc tttctcgata aagtcaatat acatctcatc
     4501 tgaacatact ctttcactta gtaaatctaa taaggcaaac gaaccgtttc tgttgtatcc
     4561 aaattttcca accataaaat aatcaccacc aactccaaag gaaggatccg caatagtaca
     4621 tgtgtaatca aactgcgtat ctgtcaataa ggctgtcgtt ggtgtccaat tttcaaacgg
     4681 attaataaac ccttcggctg ttggattttg ttgatagata gcattgaaca agtcaatgtt
     4741 catcgtttcc atatcatgca gcaattcatc aatcgggtgc gtttcttcac aaaaactatt
     4801 cccgtcatca tcaatggcag gtattgatat aacaacagaa ttttctaaat acatatttat
     4861 gacttgctga tagaaatcat tttttgcata cctcgtaccg accatgatta tttttcctat
     4921 ttcactccct ttcaacctcc cacgccacat cgtttgaaac ttcatgatat aatcggctgt
     4981 catcgtatgg ctcaacgctt cacccatgct ggcatacaag tcatcaaaga taaggaaatc
     5041 tgctcgcctg gacattacag aactttttgc agaagtgaaa aaggcattgt cattccagct
     5101 gccgtcaaac ctcattccat tcttattgtt gtttgacagc ttatgctcgc caaatacagc
     5161 cgaccatgaa tcacccatta tctcgtttga aatcgctgct tggaattttg taacattcga
     5221 atcggtgttg caaattctca taatagaatt tgactgtgtt cttgctacca actgccaaca
     5281 acctaataac cactccgtta ctttgttagc aatgtacgat tttccgctcc cctgtggcat
     5341 cgacaacaaa aggattttat tgtctgtatc aaaacagaaa tcgtgtatct gttctaatat
     5401 cttatatgtt tcaataaacg tgcgctgttg taaccaatca tcagcaactg aattcatata
     5461 attaaccaac gttggtgccg tctgaatgtt gttgtcattt gtattctctc gctcttcatc
     5521 cgttccatgt tcaattaaca atttgcctgc cgaaacatct ccctttcttg ctttatttat
     5581 cattgccaac atgacaaggt ctgcctgcga taacttctct gtttcctttg ttatcggatt
     5641 tttcccctcc tccgtcaacg tcaaccattt gcgcatcaac gttttcctgt ttagagaatt
     5701 acccctacct ttcggattgc gtatctcccc tttcttaggt ggtattatat tttttggatt
     5761 tgccatttca aattaaaatc aaattaaaaa gtacaattcg tattgttatg ttgtttttta
     5821 tttttatctt tgtggtgata atgaggaaag attatcagga ctggaaaaac cactactcaa
     5881 agcgttgatg ttagtggttt tttttatccc ataatttgtc cgttcttttt aatttcaatt
     5941 gtcgggtcga gttttcgcat acggtcaaca att
//
LOCUS       DAOPYB010000188         5922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_16674_length_5922_cov_10.376854, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000188 DAOPYB010000000
VERSION     DAOPYB010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16674_length_5922_cov_10.376854"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1597
                     /locus_tag="PKH58_11395"
     CDS             206..1597
                     /locus_tag="PKH58_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNX89680.1"
                     /translation="MKNKYVDLIEQTFEFPNQEFTVNENELSWCGIPLMDIIKQYGTP
                     LRIAYLPKIGENIQKARRMFNVAMAKVDYEGDYHYCYCTKSSHFSFVMEEVLKQGVHL
                     ETSSAFDINIMETLFEQGLLKPETFVLCNGFKRPMYVENIQSLINKGFNNVIPILDNI
                     FELDLLMKDFDQKLKVGIRIASEEEPKFDFYTSRLGVRYNDIIPYYEEKIANNQQVEL
                     KMLHFFINTGVKDTLYYWNELSKAVNLYCELKKVCPTLDSLDIGGGFPIQTYLDFEYD
                     YEYMAEEIVSQIKLICNQNGVPEPHIFTEFGSYTVGESGAMLYSIVNQKKQNDRELWD
                     MIDSSFMTTLPDTWAINQRYIFMAINNWDSEYERVNLGGLTCDSEDFYNAEVHSNAVF
                     LPKMELGREQYIGFFHMGAYQESLSGFGGIQHCLIPAPKLVIIDKDEEGEYFTKLFAK
                     EQSYKSMLKILGY"
     gene            complement(1664..2191)
                     /locus_tag="PKH58_11400"
     CDS             complement(1664..2191)
                     /locus_tag="PKH58_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89681.1"
                     /translation="MKRFTSHLICPTDAEELHRAVIELDDNGCFSRLISLDDTSVEPA
                     KTLFLDGIITPSFVSVKEICSRGTACPDLSGYCYVKMHEMMKLPETELTGKPVIFDFG
                     CSAVELLNQQSGICALSDLPLCEIINACIFRPARALSINTDICKSGTAKLIHWQNVDI
                     VNRKITPQTRLEWLK"
     gene            complement(2188..3096)
                     /locus_tag="PKH58_11405"
     CDS             complement(2188..3096)
                     /locus_tag="PKH58_11405"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNX89682.1"
                     /translation="MKSKTNFFLKITILTILAGIFVTSCQHRPSGVLNATKMENLLFD
                     MYVMEGSMSAAGIEYNDPTLKEKYYQSLLTKYDISKAEFDSSVSWYTKHPKFFERIYI
                     NVDNRLKQLTTDVEKRKFHPIDSNATNDINIWNLRTRYILTKDSTRTRLKFDIKSIGF
                     LPGDYYILSLRHRIARTDTTFNPHMVMYVNYWDGTIDSIYTKTYNDNLTRRYKLMLKA
                     RFDRKIKSVSGMLLGYDSVRGKMNVYLDSIQLLRRFYPTRQDSIRDMVDWLDTTVIPV
                     DTVPAAISSNQGKKFPGGKFPIEAPK"
     gene            complement(3080..3694)
                     /locus_tag="PKH58_11410"
     CDS             complement(3080..3694)
                     /locus_tag="PKH58_11410"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNX89683.1"
                     /translation="MSTSKKSLLLIILLLLIDQASKFYVKLHFQLGGAVDVFPWFKIL
                     FVENNGMAFGMELFGKLFLTLFRVAAVVLLAVYIRKMIKKNVRTGYILAVSMLLAGAA
                     GNIIDSLFYGVIFSASAPHQLAVLFPSGGGYESFLYGKVVDMFYFPFITDNNGETIFF
                     RYIFNVADACVTVSVAIILLFFRKELNESLESKKVPVSTDEKQN"
     gene            complement(3818..4213)
                     /locus_tag="PKH58_11415"
     CDS             complement(3818..4213)
                     /locus_tag="PKH58_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNX89684.1"
                     /translation="MTEKEKTRYSDAELEEFRDIIMEKLEKAQKDYELLRNGLSNLDG
                     NDVSDTSPTFKVLEEGASTLNKEEAGQLAQRQMKFIQHLQAALVRIENKTYGICRETG
                     KLIPKERLRAVPHATLSIEAKDGNSKRKL"
     gene            complement(4401..>5922)
                     /locus_tag="PKH58_11420"
     CDS             complement(4401..>5922)
                     /locus_tag="PKH58_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNX89685.1"
                     /translation="GDFYPADFISEGVDQTRGWFFTLHAISTMIKGSVAFKNVISNGL
                     VLDKNGNKMSKRLGNAVDPFTTIEKFGSDPLRWYMMTNASPWDNLKFDMEGIEEVRRK
                     FFGTLYNTYSFFALYANVDNFTNADAEIPVDERPEIDRWIISLLNTLVKDVSAFYNDY
                     EPTKAGRAISDFVGENLSNWYVRLNRKRYWGGEYDKDKISAYQTLYTCLETVSRLMAP
                     IAPFYADQLFLDLNNVTGKHTCESVHLTDFPECNENLIDKNLEECMQLAQQISSMILA
                     LRRKAEKKVRQPLSKAVVPAPDETTFSQINYIAGLIKAEVNVKELEVIAPDTDMDNLV
                     KKIKPNFKTLGKKYGKQMKEIAATMPSMTKQQIAEIERNGQYALILPSGEVLLTVEDV
                     EIFTEDMPGWLVANEGKLTVALDITVSDELQREGVARELVNRIQNIRKANGYEITDKI
                     EVKILRQDEINAAVEEYADYIATQTLAASIQLVEELEQPTEIDFEDYMVRLLVTKK"
BASE COUNT         1751 a         1214 c         1184 g         1773 t
ORIGIN      
        1 ataaataatt cttaatgaaa acagatgaaa catgatttaa taattaataa atatttctat
       61 ccggttaaga ttttaatacc caaacaattt gtatatttgc acgctcattc tgaaaaaata
      121 atcaatccgt cttatttgga tttagaattt ttggagcatc cgacttaatt acttttattg
      181 ttttatattt atttttcaaa ataatatgaa aaacaaatat gtagatctga ttgaacagac
      241 cttcgagttc cccaatcagg aattcaccgt aaatgagaat gaactgagtt ggtgtggtat
      301 tccgctgatg gatatcatca agcaatatgg cactccgttg cgcattgctt atctgccaaa
      361 aatcggagag aacatacaga aagcccgccg catgttcaac gtggcaatgg ctaaagtgga
      421 ttatgaaggc gattatcatt actgctattg caccaaaagc tcacactttt cattcgtaat
      481 ggaagaagtg ctcaagcagg gcgtgcatct cgaaacctca tcagctttcg acatcaatat
      541 catggaaacg cttttcgaac agggtttgct caaaccggag acatttgttt tgtgcaatgg
      601 cttcaaacgt ccgatgtatg ttgagaatat acaaagcctt atcaacaagg gtttcaataa
      661 tgtaattccg attttggaca atatcttcga actcgatttg ctgatgaaag atttcgatca
      721 gaaattaaaa gtaggtatcc gaatcgcttc cgaagaagaa ccaaaatttg acttttatac
      781 ttcgcgactt ggtgtacgtt acaacgacat catcccctac tacgaagaaa aaatagccaa
      841 caaccaacag gtagaactga aaatgctgca tttctttatt aatacgggag taaaagacac
      901 actttactac tggaatgaat taagtaaagc ggttaacttg tattgcgaac taaagaaagt
      961 ttgtcccaca ctggatagtc tcgacatcgg tggtggtttt cctattcaaa catatcttga
     1021 ctttgaatac gattatgaat acatggctga agagattgtt tcgcagatta aactaatctg
     1081 taaccagaac ggagttcccg aaccgcatat ttttacagaa ttcggatcat atactgtggg
     1141 agaaagcggc gccatgcttt actctattgt gaaccagaag aaacaaaacg accgcgagtt
     1201 atgggatatg attgacagtt cctttatgac tacgctaccc gacacctggg ctatcaatca
     1261 gcgctatatt ttcatggcca tcaataactg ggattcggaa tatgagcgcg tgaacctggg
     1321 cgggctgacc tgcgacagcg aagatttcta taacgccgaa gtacattcga atgccgtctt
     1381 cctgcccaaa atggagctgg gcagagagca atacatcgga tttttccata tgggcgctta
     1441 ccaggaatcg ctaagcggct ttggaggaat acaacattgc cttattccgg cgccaaaact
     1501 cgttatcatt gacaaagatg aagagggcga atattttacc aaactctttg ccaaagagca
     1561 aagctacaaa tccatgctga aaatactggg atactaattc agaaattatt caaaaattaa
     1621 aaccgcaact gattgactga acttcaacag attgcggttt tttttatttc aaccattcaa
     1681 gtctggtttg tggtgtgatt ttacggttta cgatatccac attttgccag tgaataagtt
     1741 ttgccgttcc gcttttacaa atatctgtgt ttatgcttaa tgctctggcc ggacgaaaga
     1801 tgcaggcgtt gattatttcg cacagcggaa ggtctgaaag agcgcaaatt ccggattgtt
     1861 ggttgagtaa ttcaacagcg gaacagccaa agtcaaaaat aacgggttta cccgtaagtt
     1921 ctgtttccgg taatttcatc atttcatgca ttttcacata acaatagccg gacaagtcgg
     1981 ggcaggcagt accacgtgaa cagatttcct ttaccgaaac aaatgacgga gtgataatgc
     2041 catccagaaa aagtgttttt gcaggttcga cagaggtatc atcgagagat attaagcgcg
     2101 aaaaacaacc gttatcgtcc agttcgatta cagcacggtg cagttcttca gcatcggtcg
     2161 gacaaatcag atgtgatgta aagcgtttca cttgggagct tctatcggaa atttcccgcc
     2221 agggaacttt ttaccctgat ttgatgatat agcagcaggt accgtatcaa caggtataac
     2281 cgttgtatcc agccagtcca ccatatcgcg gatactatcc tgacgggtag gataaaaacg
     2341 ccttaaaagc tgaatactgt ccagatatac attcattttt ccacgaaccg aatcgtaacc
     2401 caacagcata ccgctcaccg attttatctt ccggtcgaag cgggctttga gcatcagctt
     2461 ataccggcgg gtaaggttat cgttgtaggt tttggtataa atactgtcga tagtaccatc
     2521 ccaatagttg acatacatca ccatgtgtgg attgaaggtg gtgtcggtac gagcaatacg
     2581 atgccgcaat gacaggatgt aataatcgcc gggcagaaat ccgatgcttt ttatatcaaa
     2641 ttttagccgg gttcgggttg aatctttagt cagaatatat cgtgttctga gattccagat
     2701 attaatatcg tttgtcgcat tggagtcgat cggatgaaat ttacgttttt ccacatcggt
     2761 agtcaattgt ttcaggcggt tatcgacatt gatatagata cgttcgaaaa attttggatg
     2821 cttggtgtac caagagacag acgaatcaaa ttctgctttg ctgatatcat attttgtcaa
     2881 taaactctga taatacttct cttttagcgt gggatcatta tattcaatac cggcagcgct
     2941 catcgaacct tccatgacat acatatcgaa aagcaggttt tccattttag tggcgttaag
     3001 cacaccggac ggacggtgct gacaggaagt aacaaatata ccggcgagaa tggtcagtat
     3061 agttattttc agaaaaaaat tagttttgct tttcatcggt tgacacaggc actttttttg
     3121 attcgagact ttcgtttaat tccttacgga agaagagtag aattatagct acggatactg
     3181 taacacaagc atcggcaaca ttaaaaatgt accggaaaaa tatcgtctcg ccgttgttgt
     3241 ccgtaatgaa tggaaaataa aacatatcaa ctaccttgcc atacagaaaa gactcgtaac
     3301 cgcctcccga gggaaacagt accgccaatt ggtgaggtgc gctggcactg aaaattacac
     3361 cgtaaaagag cgaatcaatg atatttcctg cagctccggc cagaagcatc gaaactgcca
     3421 gtatatagcc tgtacgtaca ttttttttaa tcatcttgcg gatataaaca gcaagcagaa
     3481 caactgccgc cacccgaaac aacgtcagaa ataatttccc gaacagttcc attccgaatg
     3541 ccattccgtt attttccaca aacagaattt taaaccaggg aaaaacatcg actgctcccc
     3601 ctaactgaaa atgcagtttt acataaaact ttgaagcctg atcgatgaga agcaacagaa
     3661 taatgagcaa cagcgatttt ttggaagttg acattggcta gaaattataa attcagattt
     3721 tttgtttcat acaaaaattc gacaatcacc gtgagccggg ttgccgaatt aatgttatat
     3781 aaaaggatat tcttgtataa ttatttgaaa acgaatacta cagttttctc ttactattgc
     3841 catccttggc ttcaatactt aatgtagcat gtggtacggc acgcagacgt tctttgggga
     3901 tcagcttacc ggtctcacga caaatcccgt aggttttatt ttcgatacga accagcgcag
     3961 cctgtaagtg ttgaataaat ttcatctgtc gctgggcaag ctgtcctgct tcttctttat
     4021 tcagtgtaga tgctccttct tcgagcacct tgaaagtggg tgaagtatca gacacatcat
     4081 tgccatcgag gttactcaga ccattacgca acaattcata atctttctga gctttttcga
     4141 gtttttccat aataatatca cggaactctt ccaactcagc atctgaatat ctggtttttt
     4201 ctttttctgt cataattaac ggtatttatg gatgttattc aacatcatag aatttaagtt
     4261 tgcgaaagta agaattatat tttggaaaag caattatttt aattaaaaat agtttaacaa
     4321 tcacatacaa taatgacttt aatttatttg attaattcta cgtttgtaaa ctctttatta
     4381 tcgtaatggt tgacagcaaa ttattttttt gtcacaagca atctaaccat gtaatcttca
     4441 aagtcgattt ctgtaggctg ttcaagttct tctacaagtt gtatggaagc agcaagcgtc
     4501 tgtgtagcta tataatcagc atattcttcc acggcagcgt taatttcatc ctgtcttaga
     4561 attttcacct caattttatc ggtaatttcg taaccattcg ctttacggat attctgaata
     4621 cggtttacca gttcgcgggc aacaccttcg cgctgcagtt cgtccgacac ggtaatatcg
     4681 agagctacag tcagttttcc ttcgtttgca accagccagc ccggcatatc ttcggtgaat
     4741 atttccacat cttccacagt tagcagaacc tcacccgaag gcagaattag agcatattgt
     4801 ccgttacgtt caatttctgc aatctgctgc ttagtcatgg aaggcattgt tgctgcaatc
     4861 tctttcatct gtttgccgta ttttttcccc agtgttttga aatttggttt tatctttttc
     4921 accaggttat ccatatcggt atccggggca atcacctcga gttcttttac attgacttcg
     4981 gctttgatca atccggcaat gtagttaatc tgactgaatg ttgtttcatc gggagccgga
     5041 actactgctt ttgagagagg ctgacgaact ttcttctcgg ctttacgtcg aagcgccagt
     5101 atcatcgacg aaatttgctg agcaagttgc atgcattctt ccagattttt atcaatcagg
     5161 ttttcattgc attccgggaa atcggtcagg tgaaccgatt cacaggtatg tttccctgtc
     5221 acattattca ggtccagaaa cagctggtca gcatagaaag gagcaatcgg agccatcagt
     5281 cgggaaaccg tttccagaca agtataaagg gtctgataag cagaaatttt atctttatcg
     5341 tattcacctc cccaatagcg tttgcggttg aggcggacat accagttact cagattttca
     5401 cctacaaaat ccgaaatggc acgtcccgct ttggttggtt catagtcatt gtaaaatgct
     5461 gatacatctt tcaccagggt attcagcagg gaaataatcc agcggtcaat ctcaggccgt
     5521 tcatcaacgg gtatttcagc atcggcattt gtgaaattat ccacattggc atacagggca
     5581 aaaaatgaat aggtattata aagtgtgccg aagaatttac ggcgaacctc ttcaatacct
     5641 tccatatcaa acttgaggtt atcccagggt gatgcattcg tcatcatata ccagcgcaaa
     5701 ggatcagaac cgaatttttc gatagttgta aacggatcaa cagcatttcc cagtcgtttt
     5761 gacatcttgt tgccgttttt atccagtacc aggccgttgg aaatcacatt tttgaaagct
     5821 actgaacctt taatcatggt actgatggca tgcagggtga agaaccatcc gcgtgtctgg
     5881 tccactcctt cagaaatgaa gtcggctgga taaaaatctc cg
//
LOCUS       DAOPYB010000189         5918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_16692_length_5918_cov_7.051168, whole genome shotgun sequence.
ACCESSION   DAOPYB010000189 DAOPYB010000000
VERSION     DAOPYB010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16692_length_5918_cov_7.051168"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..672
                     /locus_tag="PKH58_11425"
     CDS             295..672
                     /locus_tag="PKH58_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89686.1"
                     /translation="MKKALVFITSCFLFAFISCIGSSNSDSTIDNGIRCSIMAEDFVK
                     SKLKYPNEVKFNTNQYVHEVEGSEAIVLNKFTAKNSFGVESNFVYKIWLSFNGGDWTE
                     INNWSYSKLIIENSDTGEKQIYR"
     gene            935..1192
                     /locus_tag="PKH58_11430"
     CDS             935..1192
                     /locus_tag="PKH58_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89687.1"
                     /translation="MNNQISPLLTEDPIPFFGIRCLDNKQAKQFLNKVAGVINDTLDD
                     SCRVSNLQTSSKTDCLFEYANGARVRILVELPELNHREEAV"
     gene            1201..1521
                     /locus_tag="PKH58_11435"
     CDS             1201..1521
                     /locus_tag="PKH58_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89688.1"
                     /translation="MTIENFTVTPEIMEVLTHWFGNDKFRKSMTSINDDMISTYMDAL
                     MDVNDIIIDVLENEQDRQKIDYSLEILKTIKELRKDINKMHASKLSLDYEQKIMNGKN
                     TDEK"
     gene            1518..1904
                     /locus_tag="PKH58_11440"
     CDS             1518..1904
                     /locus_tag="PKH58_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNX89689.1"
                     /translation="MILTFSQRRMEQRIISGIKRTTIRTDAHNRWKPGRYIHFWMHNL
                     RNITKNPYCFAVGRVRYIEDIQILPSANTVIIARAGNKLFFSSAESLNEIAVLDGFDD
                     WEDMKNWFRTDFTGRRLTFEVHTIIL"
     gene            1924..2079
                     /locus_tag="PKH58_11445"
     CDS             1924..2079
                     /locus_tag="PKH58_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89690.1"
                     /translation="MSGKDFTPPITRKEALNMIRPLRPIANDATLEEKVIAFNRLSEF
                     IELLLPE"
     gene            2178..>2434
                     /locus_tag="PKH58_11450"
     CDS             2178..>2434
                     /locus_tag="PKH58_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89691.1"
                     /translation="MEKYNHFSYGESLSRSLRFLQPGIQRNRKVLFTAPNSDTLYSIS
                     QKLSEINYPVLVAVNGFDADYSDNDADALFERAQYFFMLLQP"
     assembly_gap    2435..2534
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2535..3044
                     /locus_tag="PKH58_11455"
     CDS             <2535..3044
                     /locus_tag="PKH58_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89692.1"
                     /translation="ITFESVGDTAEFMRIQDVESVERFNSQVSAWADRVEAQLKASAQ
                     ANFGHREKDSALFPHLADSIAANMHKDKNYKMEIRSVGFSLARHGVFMHHGAGSGYGG
                     FVGSKWTDAYGTLKTTASDSIGKMDTGNRKAVHWFNDVIERNIEELVGIVADYTLDMT
                     VNLNSILIG"
     gene            3061..>3620
                     /locus_tag="PKH58_11460"
     CDS             3061..>3620
                     /locus_tag="PKH58_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89693.1"
                     /translation="MGNDFRRGIRVYLETSDYSKGISELVNQNQKLNTEYEQLKNKAE
                     ALSTAEQKRLKQLKTQLEKNAQTEVSYKQKLKETEAVLNNLSGSSYNDLIKVQNRLQK
                     EVKETVRGTQEHTTKLEQYNRVTKEVTVAKKEMNLVVGAEGNAFSRATGFVNKYWAML
                     GAGIATITGLSFTFRKLGEDVAKMDDV"
     assembly_gap    3621..3720
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3721..>5918
                     /locus_tag="PKH58_11465"
     CDS             <3721..>5918
                     /locus_tag="PKH58_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HNX89694.1"
                     /translation="EKAYLSIASSINELGANGAASEQNISEFALRIGSLPEALKPSVA
                     ETLALGAAFEESGIEAEISGRAYNIFLKQASTESGKFAQVMGISAKEVENLINTNPMD
                     FFLKFAEGMRGMDATEVAKTLDYLGVNADGANKAIGAAANSTDRFRELITLSNKAFTE
                     GTSAINEYNIKNNNLAAQLDKARKNFREVSLELGERLSPALLKSTNATSYLIKALVEL
                     PKWLNQNKLIILSLTAIWGVHTASVYASIVADKLKAFWTDKVVISVKKLGAAIKANPW
                     GLIATGVVLGITWLNKYLNKQNEVNKSQEHFLDLANKTSDFFAQQEGLQQRFNSKGSL
                     SKSQLESLSSDLSTQISDIEKIDAELKSKAKHVIETDHTLQQLNAEYNKGNKYTKLKL
                     LTSIKWREDELLGGYAEEFSKTNAMLKELKKNKQEVDKLIASKPDTTTYTPTSDPTSD
                     SSSTKNKWDAEIDKAEEVYKIELLMYSTMLKDKLISQENYEIISLQRENNFLKEKQTI
                     LEKYKQSSIDVQQAIVNNETKIQEKNKKIDTPAIKALKIDQDALNAGLQAYVKNIQDK
                     KKAEYEFAQWKKEARKKDLQDTIAIAEYAVNAVNAYHQMEADSLEAEKQRELTAAGDN
                     AEARERIEKKYAQKELDLKKKQANADMIVKIASTIASTAQAIMQAFAQLGPVAAPIAA
                     ALIGATGAMQIASIVEQRNAIMATTLDGSSSSSSASTSTPRVANSTIKTP"
BASE COUNT         1934 a         1155 c         1212 g         1417 t
ORIGIN      
        1 catacaagcc ggtacaagtt taaggactaa tttttcttaa tttaccgaac cttaccgaac
       61 cttaccgaac cttaccgaac aaaaccgaac aaaaccgaac aaaaccgcac gaaacaacta
      121 aaaagttgca tttattcact tataagttaa gtttattcac ttataagtga aattgttttg
      181 ccgaaccctg ccgatccttg ccgaaccttg ccgaacgaaa ggattgtatt ttaagattat
      241 tttgtatttt tgtgggacta acaaaaatta ttcacattaa aaaattaaat tatcatgaag
      301 aaagctttgg tatttattac ttcttgtttt ttatttgcct ttatttcgtg tataggatca
      361 tctaatagtg atagcactat tgataatgga ataagatgtt cgataatggc agaagatttt
      421 gtaaagtcaa aattaaaata tccaaatgaa gtaaagttta atacaaatca atatgtccat
      481 gaggtagaag gttctgaagc aattgtttta aataaattta ctgctaaaaa ttctttcgga
      541 gtagaaagca attttgtata taaaatatgg ttaagtttta atggcggaga ttggactgaa
      601 attaacaact ggagctatag caagctaatc attgaaaatt ctgataccgg tgaaaaacaa
      661 atttataggt aaaccagaaa aaaaataaaa ataattgcgt ttttgtattg cagattaaaa
      721 aactattctg atatttgcag taccaaaaga atttacaaag gcacgatgtg ccacaaacga
      781 gtggcttttt ttatgcctac aaacttatta aagatatggc gacgccatat atcgagcagg
      841 taagacctgt aattgtgcct ttgggttctt ttggtagcga tatattggcg tcgctttttt
      901 tatggccata aaacaaaaac caaaagaacc aaacatgaac aaccaaattt cacctctcct
      961 taccgaagat cctattccat ttttcggtat tcgatgcctg gacaacaagc aggcaaaaca
     1021 attcctgaac aaagtcgcag gagttatcaa cgacacgctc gacgacagct gtcgggtaag
     1081 taacctacag acaagctcaa aaacagattg cctattcgag tacgccaatg gcgcccgcgt
     1141 acgcatcctt gtagaattac ccgaactaaa tcatcgggag gaagccgtat gaaagactgt
     1201 atgaccatcg aaaattttac cgtcactccc gaaataatgg aagtactcac ccattggttt
     1261 ggcaacgata aattcagaaa aagtatgact tctattaatg acgatatgat atccacctat
     1321 atggatgcat tgatggatgt aaatgatatt attatcgatg tgctcgaaaa tgaacaagac
     1381 aggcaaaaaa tagactattc acttgagatc cttaaaacta taaaagaact gcgtaaagac
     1441 attaataaaa tgcacgcaag taaactcagt ttggattacg agcaaaaaat tatgaacgga
     1501 aaaaataccg acgaaaaatg atacttacct tttcacaacg ccgaatggag caacgcatca
     1561 tctccggcat caaacgcacc accatacgaa ccgatgctca caaccgctgg aaacccgggc
     1621 gatacatcca cttttggatg cacaacctcc gcaatataac caaaaacccc tactgctttg
     1681 ccgtagggcg ggtacgatac attgaagata tacagatatt gccttctgct aatacggtaa
     1741 taatagcccg tgcaggcaat aaactgtttt ttagcagcgc cgaaagtctt aacgaaatag
     1801 ccgtactgga cgggttcgac gactgggaag atatgaaaaa ctggttcagg acagatttca
     1861 ccggcagaag actaaccttt gaagtacata caatcatttt atgaacaaaa agacagaaaa
     1921 attatgagcg gaaaagactt cacacccccg ataacacgca aagaggcgtt aaatatgata
     1981 cgcccgcttc gaccgatagc caatgatgct acgctcgaag aaaaagtaat agcctttaac
     2041 cgcttatccg aattcataga attattactg cccgaatagg ttttaatttt gtattatagt
     2101 tgtgctgagc ccttgtgctt ctgtcctttg aaagcctcgt tcgcggggct ttctttgtgt
     2161 aaattaatta cacaacgatg gaaaaataca accacttcag ctacggcgaa agcctttcac
     2221 gttcgctcag gttcctgcag cccggtatcc aaagaaaccg gaaagtactg tttacagccc
     2281 ctaacagcga cactctatac tccatttcgc agaaactgtc ggagataaac tatccggtac
     2341 tcgtagccgt caacggtttt gatgccgatt acagcgacaa cgatgccgat gcactcttcg
     2401 agcgtgcaca gtacttcttt atgctcctgc aaccnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnatcacc ttcgagtcgg tgggcgatac tgccgaattt atgcgtatac
     2581 aggatgtgga atcggtcgaa cgcttcaata gccaagtatc cgcctgggcc gacagggtag
     2641 aggctcaact caaggcatcg gcgcaggcta attttggtca ccgcgagaag gactcggcgc
     2701 tgttcccaca cctggccgat agcatcgccg ccaatatgca taaggataag aactacaaaa
     2761 tggaaatccg gtcggtaggt ttctccctgg cacggcacgg ggtattcatg caccacggag
     2821 ccggttccgg ttacgggggc ttcgtcggca gtaagtggac agatgcgtat ggtacactca
     2881 agactaccgc atcggatagt atcggcaaga tggacacagg aaaccgaaaa gctgttcact
     2941 ggtttaatga cgtcatagaa cggaatatcg aagagttggt tggtattgta gccgattata
     3001 cgctcgatat gacagtcaat ctcaattcaa tattaatcgg ataaaaaaaa tacactcatt
     3061 atgggaaacg atttcaggcg cggaataagg gtttaccttg aaacctccga ctactcaaaa
     3121 ggcatcagcg aactggtcaa ccagaaccag aagctgaaca cggaatacga acaactgaag
     3181 aataaggcgg aagcattatc aactgcggag cagaagcgac taaaacaact aaagacgcag
     3241 cttgaaaaga acgctcagac agaagtaagt tataaacaga aactgaagga aacggaagcg
     3301 gttctcaata acctttccgg atcttcatat aacgacctga taaaggttca gaacaggctg
     3361 caaaaagaag taaaggaaac agtaagaggc acacaggaac acacaacgaa acttgaacaa
     3421 tacaaccgcg tgacgaaaga agttactgtt gccaaaaaag agatgaacct ggttgttggt
     3481 gctgaaggaa acgctttcag ccgtgccacc ggatttgtga ataaatactg ggcgatgctt
     3541 ggagcaggta ttgctaccat aaccggattg agtttcacct tccgaaaact tggcgaagat
     3601 gtggccaaga tggatgatgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 gagaaagcat atctttccat tgcttcatct atcaatgagc ttggggctaa cggtgctgct
     3781 tcagaacaaa atatatctga atttgcttta cggattggat cattgcctga agcattgaaa
     3841 ccatcggtag ctgaaacgct tgccttaggt gcagctttcg aagaatcggg tattgaagcc
     3901 gaaatatcgg gacgtgctta taatattttc cttaaacagg catccaccga atcgggaaaa
     3961 tttgcacagg taatgggaat atcagcaaaa gaggttgaaa atttaataaa taccaatcca
     4021 atggatttct tcctaaagtt tgctgagggt atgcgtggga tggatgctac cgaagtggca
     4081 aaaacacttg attacctggg cgtaaatgcc gatggtgcta ataaggctat tggtgctgcc
     4141 gccaatagta cggatcgatt tagggaactt attacacttt ccaacaaagc gtttactgaa
     4201 ggcacttcag cgataaacga atataacata aaaaataata acttagcggc acagctcgat
     4261 aaagcccgta aaaatttcag ggaagtttct ttggaacttg gcgagagatt aagcccagct
     4321 ctgctaaaat caactaatgc aacgtcatat ttgattaagg cactcgttga attgcctaaa
     4381 tggctaaatc aaaacaaatt aattattcta tcccttacag ctatatgggg agttcacact
     4441 gcatcggtat atgcgtcgat agttgcagat aaactgaaag cattctggac agacaaggtg
     4501 gttatttccg ttaaaaagtt aggagctgcc atcaaagcaa atccctgggg actgatagct
     4561 accggtgttg ttttgggtat tacctggtta aataaatatc tgaataaaca aaacgaggtc
     4621 aataaatcgc aggaacattt ccttgattta gccaataaaa ccagtgattt ttttgcacag
     4681 caagaaggtc ttcagcaacg ttttaactcg aaaggttctc tcagcaaatc acagcttgag
     4741 agcctgagca gtgacctctc aactcagatc agtgatattg aaaagattga tgctgagtta
     4801 aagtcaaaag ctaaacatgt aattgaaact gaccatacac tccaacaact aaacgctgag
     4861 tataacaaag gaaataaata taccaagcta aaattactta caagcattaa atggcgtgaa
     4921 gatgaattgc ttggcggata tgcggaagaa ttcagcaaga cgaatgctat gctgaaggag
     4981 ctgaagaaaa acaaacagga ggttgacaag ctgattgcat ctaaaccgga tacaacaaca
     5041 tatactccca catcagatcc tacttccgat tcatcatcaa caaaaaacaa atgggatgcc
     5101 gagatagaca aagctgagga agtatataag attgagctgc ttatgtactc aacgatgttg
     5161 aaggataagc tgatcagtca ggagaattat gaaataatat cgcttcagcg tgaaaataat
     5221 ttcctgaaag agaaacagac tatcctggag aaatataaac aatcttcgat agatgtacag
     5281 caggcaattg tcaacaacga gaccaaaatt caggaaaaaa acaagaagat tgatactccc
     5341 gctattaagg ctctaaagat agatcaggat gcgttgaatg ccgggttgca ggcttacgtg
     5401 aaaaatattc aggataagaa aaaagctgag tatgaatttg cgcagtggaa aaaggaagct
     5461 cgaaagaaag acctgcaaga tactatcgct attgctgaat atgcggttaa tgccgtgaat
     5521 gcttatcacc agatggaagc cgatagtctc gaagccgaaa aacagcggga acttactgcc
     5581 gccggtgata atgctgaagc cagggaacgg attgagaaga aatatgctca gaaggagtta
     5641 gatctgaaaa agaaacaggc caatgcggat atgattgtga agattgcatc cacaattgcc
     5701 tcaactgcac aggctatcat gcaggctttc gcgcaacttg gtccggtagc tgcgccgatt
     5761 gctgccgcgc tgatcggagc gacaggtgcg atgcagattg cctctatcgt cgaacagcgt
     5821 aatgccatta tggctaccac gctggatgga tcgtcttcgt catcctctgc atccacttcc
     5881 acaccacggg ttgcaaattc aacaataaaa acaccgca
//
LOCUS       DAOPYB010000190         5757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_17398_length_5757_cov_21.260786, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000190 DAOPYB010000000
VERSION     DAOPYB010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17398_length_5757_cov_21.260786"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5757
                     /locus_tag="PKH58_11470"
     CDS             <1..>5757
                     /locus_tag="PKH58_11470"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89695.1"
                     /translation="NFLLAGLFVISVGLGQAQNFTSPSYKKQTKSSTGKPFTPSAINK
                     NQNPETTKFNVGYSPMKVAAHAPAVNDPAYEAKKEEWMVKYPEEYNAFQQISNGIQGI
                     NPESTQGVTVLKQRVAEHAPDPDDPIYDVKKAQWIKNYPEEYKALFHSPAAVNTIVST
                     HNSNEIKQQIMPTDNRAIEYTPGYKIAIHAPEYNDADYEAKKEEWMAKYPNEYGAAQQ
                     IRPDINYTPAKATISGTGQQPQNIMMKAGEHAPYVNDPDYVQKKEQWIINYPDEYKQF
                     NSTTEKSLKEVNAKEICDQNISPIDYGKGGIFEGRNPQDEIVSLRDETSKHFRNANGA
                     ADAIITAGMPLNYHENGSWKTIIKEIVPNTTGFFPEFTYGNTTNTTKTYYSGISTGGV
                     LTEYLGNTIREWINPKISWIVNGNLTETVNITNTSGSVNLNKIIYSNCFPGTDVRFTQ
                     ENGGKKLDIILNNSALLSNIPAGASHLAICETIILPEGWTYRLSQSILFIINEKGLIV
                     FSYAAPEFYDSRPDVQKVEGKYEVKQNNNELAITTLVPTEWLTAKDRVFPIIIDPTAT
                     IYATSGGWQCSACPTQYINDVAIIYSGYLSGDHRAWAKFNTSSIADGSAVSSVTLALY
                     CDYAESSNSTTISTYDISTYYGPYGSYSVDYWNDLGDGTNYNSYTVTTAGTYGPSSLG
                     SSASSKLQGQLVSDRFQVGIVNSNTTSQSFAKRFTGSSYIVVTYGSNPTCGSTNLGNI
                     SPACQAATASYSSGTVPYWSFSATAGNTYHFSLGSNSEDSYLHLYDASFTELASNDDY
                     GPFWNGMAASLSWTCTTSGTYYIAASHYTCNTFNNSGSIAYWYTTATYESSGATITPS
                     TVWQNQAYSAGNAYWYRFQATAGLIYDFSLCDNSEDSYIRIYDQYWNFQSSADDNGPF
                     CSSVPASISWTCPTSGTYIIQISNFSCDGFINSGNLAYKFQLPCTLGCGDTDMGFLSF
                     SNCSPQDASYNAGTRPFWIFNATSGYTYNFSMGASSEDTYLRLFDLSCSQLAFEDDAG
                     PFTTGLQSSISWTCATTGTYIISASEYSCDAFVNSSYLTYWRSNDPYITTDLATIIPT
                     TSWQNQAYTSGNLSVYRFNATAGLNYDFSLCSNTEDSYIRIYNTTFDLMYSNDDNGQW
                     CTGLAASMSFNPPSTGDYIIEISHFGCNGFTNSGTLAYRYCNPPAAPSPVTASPSTIC
                     IGGSSNLNATSAGNTIQWYTDASGGSPFGTTASGVNFSVSPAVTTTYYAESQSGGTAG
                     GGAAYYIRAQSGEPWGYTNNVDAMNNVYGSGNWTLAYFENAVPATVFSAGTDFVYMDG
                     SDAGADEMETFLNANITTIQNWVSAGNYLVLKSAPNEGDGMSYGFGGTSLVYDGSLEQ
                     TYGYAVNTSHQVFNYPFTPCATSFTGNYISHAYITGSGITSLMIGENTYPMLAEKTWG
                     SGKVLFSGMTLSDWWDPETEADNLLYNIIYYIAQPSGCESTTRTPVTVSVVNDPIATA
                     SGNTSICEGNTALYTVSCTGGTGTPTYQWYFKSPSGSWSALTNGSSNAPCYASVLGAT
                     SANLTLGLYSFASACSGYQVYCVVTYSISGCNSTSSNIITTTVTPSNTITLTAGGTQT
                     KCINTAITTTTYATTGATGASVTGLPSGVTGSFASNVVTISGTPTVSGAYTYTVTLSG
                     GCGVISTTGTITVTPNNTISLTAGGTQTKCINTAITSTTYSTTGATGASVTGLPAGVT
                     GAYASNVVTISGTPTVSGAYTYTVTLNGGCGVISTTGTITVTPNNTISLTAGGTQTKC
                     INTPITTTSYTTTGATGATITGLPTGVTGSFASNVVTISGTPTISGAYTYTVTLNGGC
                     GAISTTGTITVTPDNTISLTAGGTQTKCINTAITTTTYATTGATGASVTGLPSGVTGS
                     FASNVVTISGTPTVSGAYT"
BASE COUNT         1710 a         1340 c         1178 g         1529 t
ORIGIN      
        1 aattttttgc ttgcagggtt atttgtcatt agtgtgggac tgggacaggc acaaaatttc
       61 acctcaccat cttacaaaaa gcagacaaaa agcagcacag gaaaaccttt taccccatct
      121 gcaataaaca aaaaccaaaa tccggaaaca acgaagttta atgttggtta ttctccgatg
      181 aaggttgcgg cacatgctcc tgctgtcaac gaccctgctt atgaagcaaa aaaagaagaa
      241 tggatggtta aataccctga ggaatataat gcttttcaac agatatcaaa tggcatccag
      301 ggaataaatc cggaatcaac acaaggggta acagtcttaa agcaaagagt cgctgaacat
      361 gccccagacc cagacgaccc catttatgat gtcaaaaaag ctcaatggat aaaaaattat
      421 cctgaagagt ataaagcttt atttcatagc ccggccgctg taaatactat tgtcagcaca
      481 cacaattcta atgaaattaa gcaacaaata atgccaacag acaacagagc cattgaatac
      541 acccccgggt acaagatcgc catacacgcc cctgaatata atgacgctga ttatgaagct
      601 aaaaaagaag aatggatggc aaaataccct aacgaatatg gtgctgcaca acagatacgt
      661 cccgacatta actacacgcc tgccaaggca accatatccg gaacaggaca acagccacaa
      721 aacatcatga tgaaagccgg agaacatgcc ccctatgtta acgatcccga ctacgtgcaa
      781 aaaaaagaac agtggataat aaactatccc gatgaataca aacaattcaa ttcaacaaca
      841 gaaaaaagct taaaagaagt taatgcgaaa gaaatttgtg atcaaaacat ttctcctatt
      901 gattatggga aaggcggcat ctttgaagga agaaatcctc aggacgaaat agtttctcta
      961 agggatgaaa catctaagca ttttcgcaat gcaaatgggg cggctgatgc tatcattaca
     1021 gcaggaatgc cgctgaacta tcatgaaaat ggttcatgga aaactatcat aaaggaaatc
     1081 gttccaaaca ccaccggatt ttttccagaa tttacttatg gaaataccac caatacgacc
     1141 aaaacctatt attctggcat aagcactgga ggggtgctca cagaatacct tggaaatacg
     1201 atcagggaat ggattaatcc caaaatttct tggattgtga acggtaacct gaccgaaact
     1261 gttaatatca ctaatacttc ggggagtgtt aatttgaata aaataatata tagcaattgt
     1321 ttccccggta ccgatgttcg ttttactcag gaaaatggcg gtaaaaaact ggatatcatt
     1381 ttaaataatt cagcactctt gtcgaatatt cctgctggtg ccagccatct tgccatctgc
     1441 gaaaccatta tactgcctga aggttggaca tacaggctaa gccaatcaat cctttttatt
     1501 attaatgaaa aaggtttgat tgttttttcg tatgcggctc ctgaatttta tgatagcagg
     1561 ccggatgtac aaaaagtaga aggaaaatac gaggtaaagc agaacaataa tgaattggcc
     1621 ataaccaccc ttgttccaac agagtggtta accgcaaagg atagagtatt tccgattatc
     1681 atagacccga cagcaacaat ctatgccaca tctggtggct ggcaatgttc tgcttgcccg
     1741 acacagtata ttaatgatgt tgctattatt tatagcggtt atctttccgg agatcatcgt
     1801 gcctgggcaa aattcaatac atcttctatt gcagacggct ctgctgttag cagtgttacc
     1861 cttgccctgt attgtgatta tgcagaatca agcaactcaa cgacaatcag cacttatgac
     1921 atttcaacct attatggtcc gtatggtagt tacagtgtcg attactggaa tgacctcggt
     1981 gatggcacaa attacaattc ctatacggta acgaccgctg gtacctatgg tccttccagt
     2041 ctgggatcca gtgcttcttc gaaattacag ggacaattgg taagtgaccg gtttcaggtg
     2101 ggcattgtaa attcaaatac tactagtcag agttttgcca aaagatttac tggatcaagt
     2161 tacatcgtag taacctacgg ttcaaatcct acctgtggca gtacaaacct gggcaatatt
     2221 tctcctgcct gccaagcggc tactgcttcg tattcatcag gaacggttcc ttactggtct
     2281 ttttctgcga ctgcaggaaa tacctatcat tttagtttag gttccaattc cgaggacagt
     2341 tatcttcatt tatacgatgc ttcttttaca gaattagctt caaatgatga ctatggccct
     2401 ttctggaacg gaatggccgc ttcattaagc tggacatgta caacatcagg cacttattac
     2461 atcgctgcca gccattatac atgcaacaca tttaataatt cgggcagtat cgcatactgg
     2521 tacaccaccg caacctatga aagtagcgga gcaacaatta caccgtctac cgtatggcaa
     2581 aatcaggcct actctgctgg taatgcatat tggtataggt ttcaggcaac agcaggcctg
     2641 atttatgact ttagcttgtg cgataattcg gaggattcat acatccggat ctatgatcag
     2701 tattggaatt tccaatcttc agctgacgac aacggcccgt tctgctcgag tgtccccgct
     2761 tccatttcgt ggacttgtcc aacttccgga acatatataa tccaaataag caacttcagt
     2821 tgtgatggat ttattaattc cgggaacctg gcatataaat tccaactccc atgtacactt
     2881 gggtgtggcg acacagatat gggattctta tctttttcga attgttctcc acaggatgct
     2941 tcatataatg caggaaccag accattctgg atttttaacg caacctctgg ctatacttat
     3001 aacttttcga tgggcgcaag ttctgaagat acctatttgc gtctgtttga tttgagttgc
     3061 agccagctag cattcgaaga cgatgcaggg ccttttacta cagggcttca gtcatcaata
     3121 agctggacat gtgccaccac cggaacatat attatcagtg ccagcgaata ctcctgcgat
     3181 gcttttgtca actcaagtta cctgacttat tggcgcagta acgaccctta tataactaca
     3241 gatctggcaa caattattcc gacaacctca tggcaaaacc aggcatatac cagcgggaac
     3301 ctttctgttt acaggtttaa tgccactgcg ggtcttaatt atgatttcag tttgtgcagc
     3361 aataccgagg attcgtacat caggatatac aacaccactt tcgatcttat gtattccaac
     3421 gacgataacg gacaatggtg tacgggcctc gccgcttcca tgagtttcaa tccaccttcc
     3481 actggagatt atatcattga aatcagccat tttggttgta atggttttac gaattcagga
     3541 accctggcat accgttattg caacccccct gcagcccctt cccccgtaac agcttctcct
     3601 tctacaatct gtatcggcgg ctcatccaac ctgaatgcaa cttcggcggg taacaccatt
     3661 caatggtaca cggatgcaag cggtggatca ccttttggta cgacagccag cggagtaaat
     3721 ttctctgtta gccccgctgt aaccacaacc tactatgctg aatcgcaatc cggaggtacc
     3781 gccggaggtg gcgctgccta ttatatcagg gctcaatcag gagaaccatg gggatataca
     3841 aacaatgtcg atgctatgaa taatgtttat ggctcaggaa actggactct cgcttatttt
     3901 gaaaatgccg ttccggctac agtgttttca gcgggtaccg atttcgttta tatggatggc
     3961 agtgacgctg gtgctgatga aatggaaact ttccttaatg ccaatataac aactattcaa
     4021 aattgggtca gcgccggaaa ctaccttgtg ttgaaaagcg ctccaaacga aggcgatgga
     4081 atgagttatg gttttggtgg aacctcgctg gtttatgatg gttctttaga acaaacatat
     4141 ggatatgccg ttaacacttc gcaccaggtt ttcaactatc ctttcacacc atgtgctacc
     4201 agttttaccg gcaattatat atcacacgcc tatataactg gttcaggaat taccagtctt
     4261 atgattggag aaaacaccta tccaatgctg gctgaaaaaa cctggggatc cggtaaggtt
     4321 cttttcagcg ggatgacatt atctgattgg tgggaccccg aaacagaagc cgataatctg
     4381 ctgtacaaca ttatttatta tatagctcag cctagcggat gtgaaagcac tacacgcacc
     4441 cctgttactg tgtctgttgt aaatgaccct attgccaccg cttctggaaa tacaagcatt
     4501 tgcgaaggaa acacagcatt atacacggtt tcatgcacag gtggtactgg aacaccaact
     4561 taccaatggt attttaaatc gccttcaggc tcatggagcg cccttacaaa cggctcatcc
     4621 aatgctcctt gctatgcaag cgttttaggc gctaccagtg ctaatttgac tttgggactg
     4681 tatagttttg ccagcgcatg ttcaggttat caggtatatt gtgtcgttac ttattcaata
     4741 agtggatgta attctactag ctcaaacatc attacaacaa ctgtaacccc aagcaatacc
     4801 atcactttaa ccgccggcgg cacacaaaca aaatgtatca atacggcaat tactacgacc
     4861 acctacgcta ccacaggtgc cacaggtgct tcggttaccg gattgccaag cggagtaacg
     4921 ggatcttttg cctccaatgt ggtgaccatc agtgggacac ctactgtttc tggagcatac
     4981 acctatactg ttaccctgag cggtggttgc ggagttatca gcactacagg caccatcacg
     5041 gtaacaccga ataatacaat ttctttaaca gcaggtggaa ctcagacaaa atgtataaac
     5101 actgctatta cttccactac ctattctact acaggggcta ccggagcaag cgttaccgga
     5161 ttacccgccg gagtaacagg tgcttatgct tccaatgtgg taacaatcag cggtactccc
     5221 actgtttcgg gtgcctatac ctataccgtt accctaaacg gtggctgcgg agttataagc
     5281 actacaggca ctatcacagt aacaccgaat aatacaattt ctttaacagc aggtggtacg
     5341 cagacaaagt gtatcaatac cccgataaca acaacctcat acactaccac cggagccacc
     5401 ggcgccacta ttaccggttt accaacaggc gtaacgggat cttttgcctc caatgtggta
     5461 accatcagtg gaacacccac gatatcggga gcttatacct atactgtcac actgaacggt
     5521 ggttgcgggg ctatcagcac tacaggcacc atcacggtta caccggacaa taccatctct
     5581 ttaacagcag gtggaactca gacaaagtgt atcaatacgg caattactac gaccacctac
     5641 gctaccacag gtgccacagg tgcttcggtt accggattgc caagcggagt aacgggatct
     5701 tttgcctcca atgtggtgac catcagtggg acacctactg tttcaggtgc atacacc
//
LOCUS       DAOPYB010000191         5750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_17425_length_5750_cov_11.189113, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000191 DAOPYB010000000
VERSION     DAOPYB010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17425_length_5750_cov_11.189113"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PKH58_11475"
     CDS             complement(<1..733)
                     /locus_tag="PKH58_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89696.1"
                     /translation="MSKKILFIVFLAYSSFCFAATPKFSPNSVSLYGPIKVQKPLIID
                     STDVKGKKYSDDNLLATGINFPSQEKFSNVLFVDTAGYYRLTKPATGNVFYLVSFYVN
                     SDRYAKAKIKVKSPDRFELYINDKKETEKKTTEDSLKDAKTAEADLNGNVRGTHVLLK
                     YLVSEKSKSESAFQITIEPDKQDSAAVYSFDKKGLRPITIEDILIGKRVSNVSVSPGG
                     KFVLINYNTTDNEGKVSYQVEVIETK"
     gene            complement(774..1886)
                     /gene="hisC"
                     /locus_tag="PKH58_11480"
     CDS             complement(774..1886)
                     /gene="hisC"
                     /locus_tag="PKH58_11480"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNX89697.1"
                     /translation="MNKKNDNINKSDLSTPTPPSGGWGAVISNLLRKNIKELQPYSCA
                     RDEFQGEASVYLDANESPFNGPFNRYPDPLQLELKRKLSKIKRVPTQNIFLGNGSDEA
                     IDLVYRAFCEPRIDNVVAIEPTYGMYKVCAAINDVEYRKVLLDEDFQFTAQSLLDATD
                     SHTKVIWLCSPNNPTGNALNHDEIILLLESFSGIVVLDEAYIDFSSGESYTQILYNFP
                     NLIILQTLSKAWGSAAIRLGMAYASQEIIQVFNKIKYPYNVNLLTQQQALRSLANEKQ
                     VIYWKRIILEEREKLILDLSALDMVQHIYPTDANFVLVRVDDANAVYQKLVSEGIIVR
                     NRNTVSLCEGCLRITVGTAEENQRLISELKNIDATE"
     gene            complement(2046..3326)
                     /gene="hisD"
                     /locus_tag="PKH58_11485"
     CDS             complement(2046..3326)
                     /gene="hisD"
                     /locus_tag="PKH58_11485"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNX89698.1"
                     /translation="MEIFKYPSREEWASLQARPVFDYTVLFDKVRKILDDVRENGDKA
                     VINYTLQFDNVCLDALEVTKEEIAEAAPLLSVELKQAIEMSRRNIWKFHSEQQQEFDE
                     IQTSPGVYCWQKAVPIEKVGLYIPGGSAPLFSTVLMLGIPAQIAECKEVILCTPPNSD
                     GKVHPAILYAAKVAGIHRIFKIGGTQAIAAMAYGTETVPKVYKIFGPGNQYVTAAKQL
                     VSLRDVAIDMPAGPSEVEVMADETAKSVFVAADLLSQAEHGADSQVLLVTNSEKLVEE
                     VKDQIQIQINKLPRKETAEKALENSKIIILKSLEEAIAFTNEYAPEHLIISTRSYMVV
                     AENIVNAGSVFLGNMTPESAGDYASGTNHTLPTNGYAKAYNGVNLDSFIKKITFQQIT
                     PEGLTNLSNAIILMAENEGLQAHANAVKLRTERE"
     gene            complement(3434..4153)
                     /locus_tag="PKH58_11490"
     CDS             complement(3434..4153)
                     /locus_tag="PKH58_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNX89699.1"
                     /translation="MIDEAIKSYLNKNKFPEFHLKAVFFDMDGVLFNSMPHHAIAWVK
                     AMNAVGLEFSEHEAYMNEGQTGTATINGVFVRVNGREATEEEKQDIYKLKAQYFEELG
                     TPETMPYAYDLLKKIKQKGYQIFVVTGSAQPTLIHSLKDHFPGIFVKENIISAFDVTN
                     GKPHPEPYLKALKKARVNPWEAVVIENAPLGVQASSAAGIFTIGVNTGPLPEEVLSSN
                     GAGVVLKSMRDLYEQWDNFNF"
     gene            4356..4428
                     /locus_tag="PKH58_11495"
     tRNA            4356..4428
                     /locus_tag="PKH58_11495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4389..4391,aa:Pro,seq:cgg)
     gene            4743..5270
                     /locus_tag="PKH58_11500"
     CDS             4743..5270
                     /locus_tag="PKH58_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89700.1"
                     /translation="MKFRFILPALLMIITFSACNLDNTSNTSPVVTLYSPRVNGDSIV
                     KMYFTDETQSAVRVDSLHVADTISMLVVADARANSLTEFDIVPSDTSAIKVIIPDSIE
                     DYIASGSIPKEGKYLMKPKTMLFYYPLTFIPQKPVEKLTIKFKVINDAVSVPNTSAFE
                     FSFPIKPKRIKPGVE"
     gene            complement(5386..>5750)
                     /locus_tag="PKH58_11505"
     CDS             complement(5386..>5750)
                     /locus_tag="PKH58_11505"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNX89701.1"
                     /translation="YQELLRNAKNIKEILDIEEKIRVLEEEIESTTGRLKYLNDQVNY
                     STLELNISQKKAFKYTPPFRQNFFEKLKQSLSRGWYGFVDFFFFLLSNWAILILLSVL
                     IWLWMKYRKRRKSRKQKE"
BASE COUNT         1482 a         1206 c         1279 g         1783 t
ORIGIN      
        1 gttttgtctc aatcacttcc acctgatagc ttaccttgcc ttcgttgtcg gtggtattgt
       61 agttgataag tacgaattta ccgcccggcg aaacagaaac gttcgaaacc cgtttaccga
      121 tgaggatgtc ttcgatggtg atggggcgaa gtcctttctt gtcgaatgaa taaacagccg
      181 cgctgtcttg tttgtccggt tcgatggtta tctgaaaggc tgattcggat ttgctcttct
      241 cgctgaccag gtatttgagc agcacgtgtg ttccgcgaac attgccgtta aggtctgctt
      301 cggctgtttt ggcatctttc agactatctt cggttgtttt tttttcggtt tcctttttgt
      361 cattgatata cagttcgaag cggtcgggtg attttacctt gattttggct ttggcgtatc
      421 tgtcggagtt tacgtaaaaa gatacgagat aaaacacatt tccggtggcg ggttttgtca
      481 gacggtaata tccggctgta tccacaaaaa gcacgttgct gaatttttcc tggctgggaa
      541 aattgatacc ggtggcgagc aggttgtcgt cggaatactt tttgcctttc acatctgtac
      601 tgtcaatgat caggggtttt tgcactttta ttggcccgta taacgaaact gaattcggac
      661 tgaatttggg tgtggcagca aaacagaaac tgctataagc taaaaatacg ataaatagaa
      721 ttttttttga catgattatg atattgattt agagacggtt gtaaaactga tgtttattca
      781 gtagcatcta tgtttttcaa ttcgctgata agtcgctggt tttcttcggc agttcccacc
      841 gtaatccgca aacaaccctc acaaagtgat accgtattgc ggttacgtac aatgatacct
      901 tcactcacaa gtttctggta aactgcattg gcatcatcca cacgcacgag aacgaagttg
      961 gcatcggtag ggtatatgtg ctgaaccatg tccagcgccg ataaatcaag aatcagcttt
     1021 tcgcgttctt cgagaataat tcttttccag tatatcacct gtttctcgtt ggcaagcgag
     1081 cgcaaagcct gctgttgtgt cagtaaattg acgttgtaag ggtatttgat tttattgaaa
     1141 acctgaatga tttcctgtga ggcatatgcc atgccgaggc ggatggctgc acttccccag
     1201 gctttggata aggtttgtag aatgatcagg ttcgggaaat tgtaaagtat ctgtgtataa
     1261 ctctccccgg acgaaaaatc gatatacgct tcatcgagca caacgatacc actgaacgat
     1321 tcgagcaaga ggatgatttc gtcatggttc agggcgttgc cggtggggtt gttgggtgaa
     1381 catagccaga tgactttggt gtggctgtcg gttgcatcga gcagtgattg agctgtaaac
     1441 tggaaatcct cgtcgagcag aaccttgcgg tattctacat cgttgatggc ggcacagact
     1501 ttatacatac cgtaggtcgg ctctatggca accacgttgt cgatgcgtgg ttcgcaaaaa
     1561 gcccggtaaa cgaggtcaat ggcttcgtcg ctgccgttac ccagaaaaat gttttgggtt
     1621 ggaactcgtt tgatcttgct gagtttcctc ttcagttcaa gttgcaacgg atcgggatag
     1681 cggttgaaag gtccgttgaa gggactttcg ttcgcgtcga gatataccga tgcttctccc
     1741 tgaaattcat cgcgggcgca ggagtacggc tgtaattcct ttatgttttt tcgaagtaaa
     1801 tttgaaatca cggcccccca gccccctgaa gggggagttg gagtggatag gtcagattta
     1861 tttatattat catttttttt gttcataaaa atcagcttac taaatcaata tatgtattat
     1921 cagtactcag caattcgttt actactttca ttaaaagttc ctcagggatc aatccgaaac
     1981 attttcaata attcaataac tgaattaaca gaagcgtctt aaagcccccc ttcagggggg
     2041 tagggtcact ctctttccgt tctcagtttc acagcattgg catgtgcctg caaaccttcg
     2101 ttctcggcca tgagtatgat ggcgttgcta aggtttgtaa gcccctcggg cgtaatctgc
     2161 tgaaaggtta tctttttaat aaaactgtca agatttactc cgttgtatgc ttttgcatag
     2221 ccgtttgtgg gtagggtatg attggttccc gaagcataat caccggcact ttcgggtgtc
     2281 atgtttccca ggaatacaga tcccgcgttg acgatgtttt cagctactac catataactg
     2341 cgtgtggaaa taattagatg ttcgggtgcg tattcgttgg tgaaggctat ggcttcttca
     2401 agcgatttca gtatgattat tttgctgttt tccaatgctt tttcagcagt ttccttacgt
     2461 ggcagcttgt ttatctgtat ctgtatctga tctttcactt cttccaccag tttttccgaa
     2521 ttggttacca gcagcacctg actgtcggct ccgtgttcgg cctgcgaaag cagatcagca
     2581 gcgacaaaaa ccgatttggc tgtttcgtcg gccataactt ccacttccga gggtccggcg
     2641 ggcatatcta ttgctacatc gcgcagtgaa accagctgtt tggcagcggt aacatactgg
     2701 ttgccagggc cgaaaatttt atatactttg ggaacagttt ccgtaccata cgccattgcc
     2761 gcgatagcct gtgtacctcc gattttgaaa atgcggtgga tacctgcaac cttggcggcg
     2821 tagagaatgg ctggatgtac tttgccatct gaattgggag gagtgcagag gattacttcc
     2881 ttacattcag ctatctgagc gggaatgccc agcatcagta cggtggagaa gagcggcgcg
     2941 cttccgcccg gaatataaag tcccaccttt tcgatgggca cggctttctg ccagcagtaa
     3001 acgccgggtg aggtctgtat ctcgtcgaat tcctgctgtt gctcggaatg aaacttccag
     3061 atattccgtc gcgacatttc gatagcctgt ttcagttcga ctgaaagcag cggtgcggct
     3121 tcggcaatct cttccttcgt tacttcgagg gcatcgaggc agacgttatc aaactgcagg
     3181 gtgtagttta tcacagcttt gtcgccattc tcgcgtacat cgtccaaaat tttacggact
     3241 ttgtcgaaca aaacggtata atcaaaaaca ggtcgtgcct gcagggatgc ccactcttcg
     3301 cgggaaggat atttaaatat ttccataaat caatcagtac aatatattag tacataaaaa
     3361 actcaattaa aaccggcttg taattgcccg tttataagca tttcaattca tttaacctgc
     3421 tgccggaagt aagttaaaag ttaaaattat cccactgctc atacaaatcg cgcatactct
     3481 tcagcaccac tccggctccg ttgctgctta atacctcttc gggcagtgga ccggtgttca
     3541 ctccaatggt gaaaattccg gcagccgaag atgcctgtac gccaagcggc gcgttctcta
     3601 tcacaacagc ttcccatgga tttacccggg cctttttcag cgctttcagg taaggttcgg
     3661 gatgtggttt gccattggtt acatcaaagg ccgaaatgat attttctttt acaaagatac
     3721 ccggaaaatg atctttcagg ctgtggataa gtgtgggttg tgccgaaccg gttacaacaa
     3781 atatctggta tcccttctgt ttgatttttt tcagcagatc gtaagcatag ggcatggttt
     3841 cgggggtgcc cagttcttcg aaatactgtg ctttcagttt gtagatatcc tgcttttctt
     3901 cctcggtggc ttcgcggcca ttgacgcgta caaatacacc gttgatggtg gctgttccgg
     3961 tctgaccttc gttcatgtag gcttcatgtt cggaaaattc cagtcccacc gcgttcatgg
     4021 ctttaaccca ggcaatggcg tggtgaggca tggagttgaa tagcaccccg tccatatcaa
     4081 aaaatacggc tttcaggtga aattcaggaa atttgttctt gttcagatag cttttaatgg
     4141 cttcgtcaat catgttcgaa aaatttttgg gcaaagatac aaaaaaatgt tcttttcgct
     4201 gtttatcggc caaaaatgcg gatcttttca cctgttgata agtaaatgta aatcagtgtg
     4261 ctatgcgcaa tgtgatgtga gtggttaatt tttaaataag aaatctgttt cttttttcgc
     4321 aaaaatgcat taaatttgca tccgaaatca aaaagcggga tgtggcgcag ttggtagcgc
     4381 gctacgttcg ggacgtagag gtcgcaggtt cgaatcttgt catcccgact ttacagaggg
     4441 tacttataaa caaagtgtac ccttcttttt ttgtcaaaaa atgcatagtc cttcagcaga
     4501 aaacggaaat tctgacagtc agcactgaca aaacagaaaa ataacaagcc ttcggtgtaa
     4561 atccattacc ggtaaagaag ctgttatact tccgttaatc agtcatttct gataatccgg
     4621 attattattt taaaaattat tatttttttt agaatttaac cttaatggta caaattttgt
     4681 agaataaatg gaaaatttca acgttatttt acacatactc caaatatata aaataccata
     4741 ctatgaagtt ccgatttatt ttaccagcat tattaatgat aatcaccttc agtgcctgta
     4801 atctggataa cacttccaac accagcccgg tggttacttt atattcaccc agagtgaatg
     4861 gcgacagcat cgtgaagatg tactttaccg acgaaaccca atcggctgtc agggtcgact
     4921 cattgcatgt agctgatacg atttcgatgc tggttgtggc tgatgcccgc gcaaatagcc
     4981 tgactgaatt tgatatagtt cccagcgata caagcgccat aaaggttatt attcccgatt
     5041 caattgaaga ttatatagct tcaggttcta tacccaaaga gggaaaatac ctgatgaaac
     5101 ccaaaacaat gttattttac tatccgctga cctttattcc acaaaaaccg gtggaaaaac
     5161 ttaccatcaa atttaaggta ataaatgatg ccgtttcggt accgaacacc tcggcatttg
     5221 aattttcatt tccaatcaaa cccaagcgaa ttaagccagg cgtagaataa aataaaaaag
     5281 gctgtctcaa ataaatgaga cagccttttt tattaaagta atttagctga gccacggtta
     5341 atctgattaa aggaatctac accgagagta accttacaac tgcgtttatt ctttttgttt
     5401 tctgctcttt cttcttttcc ggtatttcat ccataaccag ataagcacgg acagtaaaat
     5461 taaaattgcc cagttggata agaggaagaa aaagaaatcc acaaagccat accaacctct
     5521 tgaaagtgat tgtttcagtt tttcgaaaaa gttttgtctg aatggtggag tgtacttgaa
     5581 tgctttcttc tgcgaaatgt ttaattcgag tgtgctgtag ttgacctggt cattcaggta
     5641 tttcagtctg ccggtggtac tttctatttc ctcttcaaga acacgtatct tttcttcaat
     5701 atccaggatt tctttgatgt tttttgcatt acgtagtaat tcctgatagc
//
LOCUS       DAOPYB010000192         5727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_17528_length_5727_cov_8.854372, whole genome shotgun sequence.
ACCESSION   DAOPYB010000192 DAOPYB010000000
VERSION     DAOPYB010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17528_length_5727_cov_8.854372"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1178)
                     /locus_tag="PKH58_11510"
     CDS             complement(249..1178)
                     /locus_tag="PKH58_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89702.1"
                     /translation="MKTIGKINIIIVLIGLFFTACTKDEILREPSPLVSENSKNVFFT
                     KNNVLNYELEPTDPTKITVTVAREKSQGAATAAIKVLSNSENVFNIPASVTFADGVDT
                     TTFEVSFPTAAEGIKYSFEVSFDGEQLNPYLLKTTTISKSVTRLKWDVVGEGQFYDTW
                     TLYSAPKIQIQYSALKKTYRFASPYSRALLVEAEWDNWIGGPTTDYIKFQITPADKVT
                     YTSWYVGLNYQGNEGQPVQAFFPSYLGDIRNVTTYDVEDAKSIVKEKDANGKPLLLEI
                     QPRIYIIGVGGYGLKKMYISFPGGPDLKTLLGL"
     gene            complement(1202..2848)
                     /locus_tag="PKH58_11515"
     CDS             complement(1202..2848)
                     /locus_tag="PKH58_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX89703.1"
                     /translation="MKLINKIFILTLGLGFLASCADLNTFPEGGVLTADQKTETVLAI
                     PERLSADVNGMFSIIGKQYCVFGASSSRDDDAGYPTVCLSQDLNGPDMVSDNSNYNWF
                     SVSSQYTDRADTYANPYERWAVFYNQLKIANDILATIPADNTDPTLKVYQAQARAVRA
                     FDFLSLAPYYQFKYKGNEDKPCIPIITENMTGDPSNNPRATLKVVYERIIKDLTDAIE
                     GLEGYTRKNKSEIDQKVAYGLRARANLYMENWAAAAADADKAMAGYTPYSISQVSKPG
                     FIDANDQNWMWAIIIKPDNMPDAYPSWPSVLGSFSGDSYSAGVGCYKSINALLFDKIP
                     DTDVRKGWWVDKNLHSANLATVTWGNATGDAVAALSITDVKLPFIPYTNVKFGQYGFI
                     GNNINAGDWCIMRVEEMILIKAEATAMAGNLGAGQQILADFVKTYRDPSYTVSAPNAT
                     EFQNEVWLQRRIELWGEGFSMSDIMRLGKNIVRYKTGVESNFPDDYKFNIAATNGYLL
                     LRIPQRETNNNLGIPLSANNNEGTQPQPGDNPGLTDGVIN"
     gene            complement(2878..>5727)
                     /locus_tag="PKH58_11520"
     CDS             complement(2878..>5727)
                     /locus_tag="PKH58_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX89704.1"
                     /translation="VVAYGTAKKSSFTGSAATVDAKKLSQRSVSNITNALAGQAAGVQ
                     VTSSNGQPGTAATIRIRGIGSMSASNAPLYVIDGVPYDGSLSAINPRDIENMTVLKDA
                     AANAIYGARGANGVVLITTKKATTRDAVITVDAKWGTNSRGVPNYDVMKDPAMYYETF
                     YKALYNSKANGGSTATDAYKYADANLYAKSGLGYLVYDIPAGEKLIGTNFKLNPNAKL
                     GYSDGDYYYTPDNWYNELFDKGNMRQEYNVSIAGSSDKINFYMSAGYLDDSGIVAGSG
                     FNRYSGRAKVDYNAKKWLKVGANVAYTNYDMQAPGSQNSWGSSANLFYVSNMIAPIYP
                     MYVRNADGSIKVDHRGITVYDFGGSSTNFTRAFMPLANPGITLQLDKYHAYTDVVNSK
                     WSAIITPLEGLQLTANLGANATNQRSNSLSNQFYGGSVGSEGFVTVSHSRQIGINQQY
                     LANYKKLFGNVHNLELLAGYESYALTMQNLSGANSKLYDPYIGELDNAIQTPPQVSSN
                     TDRYVTEGYLSRVQYDYDGKYFISASYRRDASSRFAPENRWGDFGSIGASWLMSKENF
                     LIGSKWIDMLKFKASYGIQGNDNIGNYYAYIDQFTVSNSNNNYSVSFLNKGNRDITWE
                     TSYAFNTGFDFELFNRRLTGTAEYFSRKTTDLLYYKPVPISLGYSSIPTNVGSILNNG
                     VELDLNAVIFRNKDIEWTANVNATSYKNKITDLSPDKRETGIKGSNYIYKIGGSLYNT
                     YMRQYAGVDDATGKALYYLDPDNGDMTTTDVYADAKQSDLGSTLAKVYGGFGTQLNAY
                     GFDFSILFSYQLGGKMYDGTYEALMHNGDGKGTNWSMDILNAWTPENPTSDIPRLNSL
                     DVSYQYQSSRFMVSSDYLSLNSLILGYTIPKAITSKWQIASLRLYVSGDNLGLLSARQ
                     GLDPRQVLGLGSSTTSGNYSYSQLKTLSGGITLNF"
BASE COUNT         1744 a         1119 c         1029 g         1835 t
ORIGIN      
        1 cggcggttac aaagacaaaa gggtagagtt cggttttttc cttccggact tggtgcggaa
       61 atatccgtat acatttcatt gtttattgca aaaaacaatt attgagattg cactagtcag
      121 cacggtggta tatgccgtgg aggagtgatt ttttatgcac ctaaaaaaat aaatacttat
      181 agtaacaaaa aaagaggctg tctcaaattt tattttgaga cagcctccat tattttttgg
      241 aaaaagaatt ataaacctaa caaagttttt aaatcaggtc ccccggggaa tgaaatatac
      301 atttttttaa gaccatagcc cccaacgcca ataatatata tgcggggttg gatttcaagc
      361 aacaatggtt tcccattggc atctttctct tttacaatgc tttttgcatc ctcaacatcg
      421 taggttgtta cattacgaat atctccaagg taagaaggga agaaagcctg aacaggttga
      481 ccttcatttc cctgataatt gagacctacg taccatgatg tgtaggttac tttatcagca
      541 ggagtaatct ggaatttaat gtaatcagtt gtaggtcctc caatccaatt gtcccactca
      601 gcttctacaa gtagtgcccg actgtatggg ctggcaaacc ggtaggtctt tttaagagca
      661 gaatattgaa tttgtatttt aggagcgcta taaagagtcc atgtatcata gaactgaccc
      721 tcaccaacta catcccattt aagtctggta actgattttg aaattgtagt ggttttaagt
      781 aaatatggat ttaattgctc tccgtcaaat gaaacttcga atgagtattt aattccttct
      841 gcagcagtag ggaaagatac ttcaaaagtt gtggtatcta caccatctgc aaatgtaacg
      901 cttgctggaa tattaaatac gttttctgaa ttactaagaa cctttatagc agcagtagca
      961 gcaccttgtg atttttcacg ggctactgta actgtaattt tagttggatc tgttggttcc
     1021 agttcataat taagtacatt gttttttgtg aaaaaaacat tttttgaatt ctctgaaacc
     1081 agtggagatg gctctctcag aatttcatcc tttgtacatg ctgtaaagaa tagccctatc
     1141 agaactatta ttatgttaat ttttcctatt gttttcatat agatattttt attgtattaa
     1201 attaattaat aacaccatcc gttaatccgg gattatcccc aggttgaggc tgtgtacctt
     1261 cattattatt tgctgacaat ggaataccta agttgttatt agtttctctc tgaggaatac
     1321 gaagcaacag atatccatta gttgctgcaa tattgaattt atagtcatca gggaaatttg
     1381 attcaactcc ggttttgtaa cgaacaatat tttttcctaa acgcataatg tctgacatgg
     1441 aaaaaccttc accccaaagc tcaattcgac gctgaagcca tacttcgttt tgaaattcag
     1501 tagcgttggg agccgaaact gtatagctgg ggtcacgata ggttttaaca aagtcagcta
     1561 aaatctgctg tcctgctcct aagttgcctg ccatggctgt tgcttcagct tttatcagaa
     1621 tcatttcttc tacacgcatt atacaccagt ctccggcatt gatattgttt ccaataaatc
     1681 catactgacc gaattttaca tttgtataag gaataaaggg caacttaaca tcggtaattg
     1741 ataaagcagc aacagcatct cccgttgcat ttccccaggt tacggttgct aaattagccg
     1801 aatgtaaatt tttgtcaacc caccatcctt tacgaacgtc agtatccgga attttatcaa
     1861 acaaaagtgc atttattgat ttgtagcaac caacaccggc agaataagaa tcacctgaga
     1921 atgaacctaa tacagaaggc caggaaggat aagcatcagg catgttgtca ggtttaataa
     1981 taattgccca catccagttt tgatcattag catcgataaa tccaggttta gatacctgac
     2041 tgatagaata aggagtatag ccggccattg ctttatctgc atctgcagca gctgcagccc
     2101 aattttccat atacaaattg gcacgtgcgc gtaatccgta tgcaactttc tgatctattt
     2161 ctgatttatt ctttcttgta taaccttcca aaccttcaat agcatctgtc aaatctttaa
     2221 tgatgcgttc atataccact tttaaagtag ctctcggatt attagaagga tcgcctgtca
     2281 tattttcagt gataattgga atgcagggtt tgtcttcgtt tcctttatac ttgaattggt
     2341 aataaggtgc taatgataaa aagtcaaatg ctcttacagc acgcgcctgt gcctgatata
     2401 ctttcagagt tggatcggta ttatcagcgg gaattgttgc cagaatatca tttgcgattt
     2461 ttagctgatt ataaaaaaca gcccagcgtt catatggatt tgcataagtg tcagctctat
     2521 cggtatattg actgctaacc gagaaccagt tataattgct gttatcactt accatatcag
     2581 gaccattcaa atcctgtgaa agacatactg taggatatcc ggcatcatca tctctggagg
     2641 atgaagcgcc aaaaacacaa tattgtttgc caataattga gaacataccg ttaacatcag
     2701 ccgataatct ttcaggaatg gctaatacgg tttctgtctt ctggtcagca gtaagaacgc
     2761 caccctccgg aaaggtattt aaatctgcgc aggaagccag aaaacccaga cccagagtca
     2821 aaatgaatat tttatttatt aatttcataa aattattttt tttaattgtt tagattatta
     2881 aaaatttaaa gttataccac cagaaagtgt ttttaactga gaataactat aattgccgga
     2941 cgtagtagaa ctacctaaac ctaaaacctg acgtggatcc aatccttgac gtgcagatag
     3001 taatccaaga ttatcgccgg atacatacaa acgtaaactg gcaatttgcc atttgcttgt
     3061 aattgctttt ggtattgtat agcccagaat aaggctgttc aaactcaaat aatcagaact
     3121 aaccataaaa cgggaagatt gatactgata ggatacatcc aaagaattca atcgtggtat
     3181 gtcagaggtt gggttttctg gtgtccaggc attcaatata tccatagacc agtttgtacc
     3241 ctttccatcg ccattgtgca ttaaggcttc ataagttccg tcatacatct tacctccaag
     3301 ctggtatgaa aacaggatgg aaaaatcaaa accgtatgca ttcaattgag taccgaaacc
     3361 accatatacc ttagcaagtg tgcttccaag gtcagactgt tttgcgtcgg catatacatc
     3421 tgttgttgtc atatcaccat tatctggatc aagataatat aatgcttttc cggttgcatc
     3481 atcaacgcct gcatattgtc tcatgtaagt attataaaga gatccaccaa ttttgtaaat
     3541 ataattactt cccttaatac ctgtttctct tttatcagga gataagtcag taatcttatt
     3601 tttgtaactg gtagcattta cattagcagt ccactcaata tctttgttgc ggaaaataac
     3661 agcattcagg tctaattcta ctccgttgtt caaaattgaa cctacattgg taggaattga
     3721 agaataacct aacgagattg gaactggttt gtaataaagc aaatctgtag tttttctgct
     3781 gaaatactct gctgtaccgg tcagtcttct gttgaacagt tcaaaatcaa aaccggtatt
     3841 aaatgcataa gaagtttccc aggtgatatc cctgtttcct ttgtttaaaa atgaaaccga
     3901 gtaattatta ttgctgttac ttactgtgaa ttgatcgata taggcgtaat aattaccgat
     3961 attatcattt ccctgaatac cataacttgc tttaaacttt aacatatcaa tccatttaga
     4021 gccaatcaaa aagttttctt ttgacatcaa ccaggaagcg ccgatacttc cgaaatcacc
     4081 ccaacggttt tccggagcaa aacgggaaga ggcatctcta cgataagaag cgcttataaa
     4141 gtatttacca tcatagtcgt actgaacacg tgataagtaa ccctctgtta catatctatc
     4201 agtattagaa ctaacctgag gtggtgtttg aattgcatta tccaattcgc caatatatgg
     4261 atcgtagagt ttggagttag ctccggataa attttgcatt gttaatgcat aagactcata
     4321 gccagccaac aactctaaat tatgtacgtt accaaacagt tttttataat ttgctaaata
     4381 ctgttggtta atcccgattt gacggctgtg tgaaacagta acaaatcctt cactacccac
     4441 actaccacca taaaattggt tgcttaagct attcgaacgt tgatttgtag cattggcacc
     4501 aagatttgct gtcagttgaa gtccttcaag tggagtgata attgctgacc attttgaatt
     4561 aacaacgtct gtatatgcat gatatttatc aagttgtaaa gtaatacccg ggtttgccaa
     4621 tggcatgaaa gcacgtgtga aattagtgga tgaaccacca aagtcatata cggtaatacc
     4681 tcgatgatct actttgatag aaccatctgc attgcgaacg tacataggat aaatcggagc
     4741 aatcatattg ctgacataaa acaagttagc agatgaaccc caagagtttt gtgatccggg
     4801 tgcctgcatg tcataattgg tataagctac attagcgcct actttaagcc atttttttgc
     4861 attatagtca acttttgcac gaccagaata acgattaaat cccgaaccag caacaattcc
     4921 actatcatct aagtaacctg cagacatata aaagtttatc ttgtctgatg aaccagctat
     4981 agaaacattg tattcttgtc tcatattgcc tttatcaaaa agttcattat accaattgtc
     5041 aggtgtgtaa taataatcgc catcagaata tcccaattta gcatttggat ttaatttaaa
     5101 attagttcca atcaattttt ctccagcagg tatgtcataa actaaataac ccagtccgct
     5161 ttttgcgtat aaatttgcat ctgcatattt atatgcatcg gttgcggttg atccaccatt
     5221 tgctttagaa ttgtataatg ctttatagaa tgtttcataa tacattgctg gatctttcat
     5281 aacgtcataa ttaggaactc cgcgtgaatt tgttccccat ttagcatcaa cagttattac
     5341 tgcatctctt gtagtagctt tttttgttgt gatcaataca acgccatttg cgccacgtgc
     5401 accgtaaatt gcatttgcag ccgcatcttt taatacggtc atgttttcaa tatcacgcgg
     5461 gtttatagct gataagctac catcgtatgg aacaccgtca attacatata acggggcgtt
     5521 actggcactc atcgaaccaa taccacgtat acgaatggtt gctgcagttc ctggctgtcc
     5581 gttactggag gtaacctgaa caccagctgc ttgtccggcc aaggcatttg taatatttga
     5641 aactgatcgt tgcgatagtt tttttgcatc cactgttgct gcagaaccag tgaaggaaga
     5701 ctttttggca gtaccataag ccacaac
//
LOCUS       DAOPYB010000193         5702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_17649_length_5702_cov_9.822029, whole genome shotgun sequence.
ACCESSION   DAOPYB010000193 DAOPYB010000000
VERSION     DAOPYB010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17649_length_5702_cov_9.822029"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..677)
                     /locus_tag="PKH58_11525"
     CDS             complement(69..677)
                     /locus_tag="PKH58_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89705.1"
                     /translation="MTKDILFGWAVASIGKSRLDMRPGEYILEANTTNDPNTSITSTW
                     SVTGPSAALVDFPYPDDAGYADKAGRLKVLEVYTPGYYTVCATANDGNYTTQPYCTDF
                     YIDDYKPAGGMFSLSPNPATTSVTITIDETKLLNATTQRTVTNPKAGYWQIQLWNSFG
                     LIKTLQTDQKKSQLDLSGLSPGFYYVLVIRDGQTYRRQLMVQ"
     gene            complement(714..1904)
                     /locus_tag="PKH58_11530"
     CDS             complement(714..1904)
                     /locus_tag="PKH58_11530"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89706.1"
                     /translation="MVWNNVKSTNGNVPKGTITLTVYNKNYINQIVDNGSTDQKIKSL
                     NDILPQTPQSAETAINYGIQTIRVTGQSINYPGLTYPNGMPIPVNKYEWKVPAGWSVN
                     GGTKTASGTYTSIYSIVDITTDYGSVGGVEVRGVNDCYGSDDYTVYSFPVNFTRKGLG
                     VLEYPKTIIAGQANTYSVTVTPISGATYEWQAPAGWSINNGGNTYTGTENIISVRNGI
                     CMTDQIIKVRYKGSSQIYSEWVEVPSMMIAPVINIPSELKQYHSAIFSLNLPDENVSS
                     VTWKVNGVNAANVVNTSTAQLTINQKGTVSVSAVLSLKSCTEMITIPAMNVNVEETVF
                     SISGPSTLYSQATYTIDNLPPGAAVQWSVTQPYIAPTISVQNSGTVILTNHYGIDGIG
                     THGT"
     gene            complement(2173..2913)
                     /locus_tag="PKH58_11535"
     CDS             complement(2173..2913)
                     /locus_tag="PKH58_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89707.1"
                     /translation="MKKKMILLIWLTTIPFIGFSQDFRKIGIGFGYHTTSIVGDSVRP
                     FELSLRYRINNKHTLQVYAPLWLKKDNQKIEHWADFTKDEHKKMYKQIYTHSLWGIGL
                     GYDYTFYSYSQFNFFTGISTDFQRYEYRNDYYDINYIQLGPIDNRLPVYDDFYKKIET
                     SYYWDRKTGISIIPNTGIRFVTEKIAVEAKLGLYFSKFDMEAFYLGKEKGLFDEEWMG
                     WWKSYYPDKSKQEYTIRPNISIALSYYL"
     gene            complement(3340..4602)
                     /locus_tag="PKH58_11540"
     CDS             complement(3340..4602)
                     /locus_tag="PKH58_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNX89708.1"
                     /translation="MFKLFRNIDNKAHKPQFGGLDIFKYIGPGLLVTVGFIDPGNWAS
                     NLAAGADFGYALLWMVTLSTIMLIMLQHNVAHLGIATGLCLSEAATIHMKPRYSKLLL
                     VSGMGASISTSLAEILGGAIALNMLFDIPIRAGALLVMIFVLIMLFTNSYKAIEKWII
                     AFVSIIGLSFLYELSLVNIDWNSAIAGWVTPSFPKGSMLVIMSVLGAVVMPHNLFLHS
                     EVIQSRQWNRQDEKIIKKQLKYEFFDTLMSMLIGWAINSAMILLAAATFFQSKTPVSE
                     LQQANQLLAPLLGNNAAIIFAVALLFAGVASTVTSGMAAGTIFAGMFKEPYDIKDNHS
                     RTGVGISLVIAFLIILLISNPFKGLIISQMILSIQLPITIFSQVYLTSSEKVMGKYKN
                     RTSTKFMLYGLGAVVSILNIALFISFFV"
     gene            complement(4817..>5702)
                     /locus_tag="PKH58_11545"
     CDS             complement(4817..>5702)
                     /locus_tag="PKH58_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044272 - sulfur compound biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative 4-mercaptohistidine
                     N1-methyltransferase"
                     /protein_id="HNX89709.1"
                     /translation="HNLIKGGSWISTGNEATYYARYAFRRHFYQHAGFRLVQSEVPLV
                     IQTNEYETDEEVTLSCEANWGDKLFTTAPNFSNALAKLVKEVTGENPQLRVLDLNADT
                     GRLAFELAQHYTDVTALDFTARMIRMAIQLQEQGFMRYVTKDEGELLLYRDVVLTDFG
                     LEKGRENILFMQADAMNLKPIYTGYDVIVVPNLLEELSNPKLFLENIHERLNEHGWFI
                     ITSTYDWDETRTRRENWPGGFKQDGEPVTSLDGIKAILSEHFTLENEPIDLTLSLRKS
                     SRITENRISQITVWKKRG"
BASE COUNT         1603 a         1185 c         1187 g         1727 t
ORIGIN      
        1 tagcaaaaga ggcaaattcc ggttagggaa atgcctcttt ttatttttag aaatgattgt
       61 tgtctgtttt attgcaccat cagctgcctg cggtaggtct gcccatccct gataaccagc
      121 acataataaa atccgggtga taagccgctc aaatccagtt gagacttctt ctggtcggtt
      181 tgaagggttt ttatcagccc gaaggagttc cagagctgga tttgccagta accagccttc
      241 ggattagtga cagttctttg ggtggttgca tttaataatt ttgtttcatc gatagtaatc
      301 gtcacagatg tggtagccgg atttggagag agtgaaaaca tgcctccggc tggtttataa
      361 tcgtcaatgt agaaatcggt acaatacggt tgggtggtat aatttccatc attggcagta
      421 gcacacacgg tataataacc cggtgtataa acttccagta cttttaatcg tccggcttta
      481 tctgcgtaac cggcatcatc cggataaggg aaatcgacta atgcagccga aggaccagtc
      541 actgaccagg tagaggttat tgacgtgttt ggatcgttgg ttgtattggc ttcgaggata
      601 tattctccgg gtctcatgtc cagccttgat ttcccgatag aggcaacagc ccagccaaac
      661 aggatatctt tggtcaatgt tatctgctga tttccaacgg ttaccactgc ctgttaagtg
      721 ccatgagtgc caatgccgtc tattccatag tggttggtaa ggattacagt gccggaattc
      781 tgaacggaaa ttgtaggagc tatatacggt tgagttacac tccattgaac cgcagcgccc
      841 ggaggcaggt tatcgatggt gtaggtggcc tgggagtaga gggtagaggg accggaaatg
      901 gaaaaaactg tttcttctac atttacattc attgctggta ttgttatcat ttctgtgcaa
      961 cttttcaaac ttaacactgc cgacacactg actgttcctt tttgatttat cgttaattga
     1021 gcagtacttg tgttaactac gttagctgca ttcacaccgt ttactttcca ggtgactgaa
     1081 cttacatttt catccggaag gtttaatgaa aaaatagcgg aatgatactg ttttaattca
     1141 gaaggtatat tgataacagg tgcaatcatc atactcggca cttctaccca ttcgctataa
     1201 atttggctgg atcctttata tctaactttt ataatctggt cggtcatgca gattccattt
     1261 ctgactgaaa taatattttc agttccagtg taagtgtttc ctccattatt gattgaccaa
     1321 cctgccggtg cctgccattc atacgtagcg ccggaaatag gcgttactgt gacagaatag
     1381 gtatttgctt gccctgcaat tatcgtttta ggatattcaa gcacacctaa gcctttacgg
     1441 gtgaaattaa caggaaaaga ataaacagta taatcatccg aaccataaca atcatttact
     1501 cctcttactt ctactccgcc cacacttcca taatctgtag taatatcaac gatggaatat
     1561 atacttgtat acgttcctga agcggttttt gttcctccat ttaccgacca accagctggt
     1621 actttccatt cgtatttgtt tacaggaata ggcataccgt ttggatatgt aagccccggg
     1681 taattaatcg attgtcccgt tacccggata gtctggatac cataatttat agctgtttca
     1741 gcagattgag gcgtttgagg cagtatatca tttaaacttt ttattttttg atctgtgctt
     1801 ccattgtcta caatctgatt tatataattt ttattataca cagttaatgt aatggttcct
     1861 ttcggcacgt tcccgtttgt ggatttaaca ttgttccaaa ccactattac attcgcgcaa
     1921 ttcgtttcag atatctccgt tacattttgt ccccactttt taaagtaccc gccggtaatc
     1981 ttccattcat acttaccata aatatctttc gtatctaccc agtattgata ttctgtttta
     2041 ggacaggcaa aattttctaa ttctgcattg ctttatgggt attgttgcgg caaccaatcc
     2101 ttgtaaaaat aagcttaagg ctactaagca aattatattt ctagagttgt tcatagctat
     2161 agtgtgtaaa agttataaat aataagataa tgctattgaa atgtttgggc gaatagtata
     2221 ttcttgttta cttttatctg gatagtacga tttccaccag cccatccatt cttcatcaaa
     2281 caagcctttt tctttaccca aataaaaagc ttccatatca aatttggaga aataaagtcc
     2341 taattttgct tctactgcaa ttttttctgt aacaaaacga atacctgtgt tagggataat
     2401 acttattcct gtctttctat cccaataata tgatgtctct atctttttat aaaaatcatc
     2461 atatacgggt agtctattat ctataggtcc taattgtata tagttgatat cataatagtc
     2521 atttcgatac tcgtaccttt gaaaatcagt acttattcca gtaaaaaaat tgaattgaga
     2581 gtaagaataa aaagtataat catagcccaa accgataccc catagactgt gtgtatagat
     2641 ttgcttatac attttcttat gctcatcctt tgtaaaatcc gcccaatgtt ctatcttttg
     2701 attgtctttt tttaaccata gcggtgcata tacctgcagt gtgtgcttgt tgttaatacg
     2761 ataccgcaag gacaattcaa agggtcgtac actgtcacct actatagaag tagtgtggta
     2821 accaaatcct atccctatct ttctgaaatc ttgggaaaat ccaataaatg gaattgttgt
     2881 cagccagatt aataaaatca ttttcttttt catgtgtaat tagaattttt aagttattaa
     2941 taaaagtatg tttcttatac aatggatttt ttactctata tagttgtatt ctgagtatta
     3001 agcgtttcgt aaaaaacttg cagtaaatat acagataaat aacaataaag gcaagccgaa
     3061 aaaagatgtt attaagcatg gaagaagtgc ataaggagca tattcatttg ttaaaatcgg
     3121 aggttttatc ctgttaattc ctttttcggg aggaaattgt ataacggatt acaaagtaaa
     3181 atctggggaa cttcggattt gaaaatccat attccgaagt gctgtaacac acttccctac
     3241 tgagagcaaa agaggcaaat tccggtgaag gaaatgcctc ttaaattata catcaatacg
     3301 tgcattcagg attagaaatc cccgcgccgt atgagctgtt tatacgaaaa aactgatgaa
     3361 taaagcaata ttcagtatcg aaaccacggc tcccagccca tacaacatga attttgtgga
     3421 ggtgcggttt ttgtatttcc ccatgacctt ttcggaggag gtgaggtaaa cctgcgaaaa
     3481 tatggtgatt gggagttgaa tgctcaggat catttgcgaa ataatcagac ctttgaacgg
     3541 attgctgata agcagaatga tgagaaatgc tatcaccagc gaaatgccta cgccggtacg
     3601 cgaatgatta tccttgatat catagggttc tttgaacatt cccgcaaaaa tagttccggc
     3661 tgccatacct gatgttacgg tagaggcaac tcctgcaaaa agtaacgcca cagcaaaaat
     3721 aatggctgca ttgtttccga gcagcggcgc gagcaactga ttggcttgct gcaattccga
     3781 aaccggcgtt ttggattgaa aaaaagtggc tgctgccagc aggatcatgg cgctgttgat
     3841 agcccagcca atgagcatgg acatcagggt atcgaaaaat tcgtatttca gctgcttctt
     3901 gattatcttt tcatcctgcc ggttccactg acggctctgg attacttcgg agtgaaggaa
     3961 caggttgtga ggcatcacca cggctcccag cacactcatg attaccagca tagaaccttt
     4021 ggggaaactc ggtgtcaccc accctgctat ggcgctgttc cagtcgatat tcaccagcga
     4081 aagttcgtac agaaatgaca gcccgatgat ggagacaaag gcaataatcc atttctcaat
     4141 ggctttgtag gagttggtga agagcataat cagtacaaaa atcatcacca gcagcgctcc
     4201 ggcccggatt ggaatatcaa aaagcatatt cagcgcaatg gcgccaccca gaatttcggc
     4261 cagtgaagtg gaaatggaag ctcccatacc cgaaaccagc agcaattttg aatagcgggg
     4321 tttcatgtga atggtagctg cttccgaaag gcaaagcccc gtggcaatac ccaggtgagc
     4381 cacgttgtgc tgcaacatta taagcatgat ggtggagagt gtaaccatcc agagcaaggc
     4441 gtacccaaaa tcggcaccgg cagccaggtt ggaagcccag ttgcccggat cgataaaccc
     4501 aacggtaacc agtaatcccg gaccgatgta tttgaagata tccaacccgc cgaactgcgg
     4561 tttgtgtgct ttattatcaa tatttcgaaa aagtttgaac ataaatatgg tgtttaaatg
     4621 aagtaaggtg gcccccaacc ccctgaaggg ggagttaaag tgcaaaaatc taagttaatg
     4681 cgaaaaattg aaaaaaatat ttgttattta gaaataaatc ccacattaat aaatcaacaa
     4741 caaatctcca cactgaaaat cccattctta ttcccccttt agggggttag ggggccggga
     4801 tttagggggc ttatcactat ccccttttct tccaaaccgt aatttgcgat attctgtttt
     4861 cggttattct ggatgatttc cggagcgaca aagttaagtc aattggctcg ttttccaacg
     4921 taaaatgttc gctgagaatt gctttaattc catcgagcga agtgaccggt tcgccgtcct
     4981 gtttgaagcc gccgggccaa ttttcacgcc gggttcgggt ttcgtcccag tcgtatgtgg
     5041 aagtaatgat gaaccatccg tgttcgttca aacgttcgtg gatattttcc aggaataatt
     5101 tcggattgct cagttcttcc agcagattgg gaaccactat cacatcgtaa ccggtgtaga
     5161 tgggcttcaa attcatggcg tcggcctgca taaagaggat attttccctg cccttttcca
     5221 gtccgaaatc cgtcaacacc acatcgcgat acaggagcaa ttcgccttcg tctttggtca
     5281 cgtagcgcat aaaaccctgt tcctgaagct ggatagccat ccgtatcatc cgcgctgtaa
     5341 agtcaagtgc agttacatcg gtgtaatgct gtgccagttc aaaagccagt cgtccggtgt
     5401 ctgcattcaa atcgagcacg cgcaactgcg ggttttcacc ggtcacttct ttcaccagtt
     5461 ttgctagtgc gttcgaaaaa ttcggcgctg tggtaaacaa cttatcaccc cagttagctt
     5521 cgcacgagag cgttacctct tcgtcagttt catattcatt ggtttgaatg acaagaggca
     5581 cttccgactg taccagtctg aaaccggcat gttgataaaa atggcggcgg aaggcatagc
     5641 gggcataata agtggcttca ttgccggtcg aaatccagga accgcccttt atcaggttgt
     5701 gt
//
LOCUS       DAOPYB010000194         5698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_17660_length_5698_cov_9.325713, whole genome shotgun sequence.
ACCESSION   DAOPYB010000194 DAOPYB010000000
VERSION     DAOPYB010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17660_length_5698_cov_9.325713"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1265)
                     /locus_tag="PKH58_11550"
     CDS             complement(21..1265)
                     /locus_tag="PKH58_11550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX89710.1"
                     /translation="MKGNNKKWRELFVTNFLGVFNDNFLKNSIIFISVTWSLPTWLSQ
                     SQLISLVASCLVVPYLIFSPIGGSLAVKYSKLKVLRLLKLIEVPVMLLACVAFYKQWV
                     LLAVLSVFALGTQSCLYSPSKYSLIRDIGGEDGVSYGSGMFETMAFMGILIGTFIASL
                     ISDHYSLLLFASIGIGVAFLGFYFAKKIRAEELPVEMTGSSTINPYKFVKSNYKFADR
                     FKLVNSAVFGTSAFWLIANMVQMNIIVHCKGYYHISNSLTGVVMACAAIGIALGTGIT
                     GKIMGTRVLKGLIIPALIGIITILLVIVFIPLSFTAFIAMIILFAFTAGMFQVPCMAL
                     MQQADLGRKRGDTVAYLNMMNFVFILIGTLVFSVTTALTDENSFAVFGVMVVLCLFIL
                     IYFLVRYPDFRNETAVLLKRNK"
     gene            1529..3046
                     /locus_tag="PKH58_11555"
     CDS             1529..3046
                     /locus_tag="PKH58_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX89711.1"
                     /translation="MKKSTIWVLMIMMTITFSALILVQAKFLKATSEMAEKQFDEAVK
                     RSMIQTANLLEENEALEYLAQTLDDNEYVQSESKSNNFVINGQKDTLKSNWKSSSSIS
                     PRIRLSTGHGKATIEETSKYLQQKFQSNFSRSKTILDQAVFRWLRENENKEISERVDF
                     TDLNETLETYLKNNNVDLPFYYTIVDNRNKIIYQCNKTINTNLRPNTDIYVQELFPHE
                     DSYRKAYMQVVFPTKQSFIYESLNLLLPSVGVIVFTFIIFVLTIVIIFRQKQLNNMKN
                     DFVNNMTHEFKTPISTISLASQMLQDPGVGKNPDMMKHVTNVIRDETKRLSLQVEKVL
                     QMSMFEKEKSTLKFTEIHINNLIKDIIGSFSLKVTSKGGKINSSLKAQSDIAYVDEIH
                     FTNIIFNLMDNALKYSDKPLILNLETWNEKDNLLISVEDNGIGMKREDLKRIFDKFYR
                     VSTGNLHNVKGFGLGLAYVKKMVTDHNGSIKVESEPGIGTKFTIIIPTLKKNNYE"
     gene            3039..3746
                     /locus_tag="PKH58_11560"
     CDS             3039..3746
                     /locus_tag="PKH58_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX89712.1"
                     /translation="MNDEKVKILLCEDDENLGMLLREYLQTKGFYCDLQPDGEAGYKS
                     FSRNKFDLCVLDVMMPKKDGFALAADIRAMNTEVPIIFLTAKSMKEDILQGFKLGADD
                     YLSKPFSMEELLYRIESILRRVRGKKSKDVVTYQIGGFTFDAQKQTLEFEGEIKKLTT
                     KESELLNLLAANANNILERNFALKTIWVDDNYFNARSMDVYITKLRKLLKDDQNVAII
                     NIHGKGYKLIMPQVKED"
     gene            complement(3798..5639)
                     /gene="mutL"
                     /locus_tag="PKH58_11565"
     CDS             complement(3798..5639)
                     /gene="mutL"
                     /locus_tag="PKH58_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNX89713.1"
                     /translation="MSDIIHLLPDSVANQIAAGEVIQRPASVIKELVENSIDAGAKNI
                     QVLIKDGGSTLIQIVDDGVGMSETDARMSFERHATSKIKDAADLFTLRTMGFRGEALA
                     SIAAVAQVELRTRKETEELGTLIEIAGTRVFRQESVQCNKGTNIAVKSLFFNVPARRR
                     FLKSPSTEMTHIRNEFYRIVLVHPDISFSLYDGTLELMSLPATSLKARIEHVFNGVSK
                     KKLEQQLLPIETNTSILSVTGFVGRPEYAQKLANQYFFVNGRYMRHPYFHKAVMVAYN
                     QLIKPNENPNYFIYFDIDTQSIDVNVHPSKTEIKFENEQAIWSILLASVKEALGKFQV
                     MPPLDFDQDDSPEIPVLDSHTAIRPPGTHFDPSYNPFRLSSQPKRQQLDWEQLYGEFK
                     KEIYQPEQQSVQPEADDHFETRLEPEQPMLDLAVNSMEFYQFKNKYILTTVKSGLMII
                     DQHRAHKRVLFDRFMKKTESQKGITQQVLFPEILELSKDEFGVMEQILPDLSWVGFDI
                     VPIGKSEYAINGIPSEIEMTSTLQLLKDMIATVSETEITVKESIKEEIAIQITRHTAI
                     QYGQTLNLTEMRELFDNLFTCENHMYTPDGKLILSMIQTEDIENKFK"
BASE COUNT         1925 a         1083 c          990 g         1700 t
ORIGIN      
        1 ttattgtata tcgtataata ttatttattt cttttgagca aaactgctgt ttcgtttctg
       61 aaatcaggat aacgaaccag aaaatatatc aaaataaaaa gacaaagtac aaccattacc
      121 ccgaagacag caaaactgtt ttcatctgtc agcgcggtcg taaccgaaaa tacgagtgta
      181 ccgataagta taaaaacaaa gttcatcata ttcagataag caaccgtatc ccctcttttt
      241 ctacccaaat cagcctgttg cattagtgcc atgcagggta cctgaaacat acccgctgta
      301 aatgcaaaaa gtataatcat tgcaataaaa gcagtaaaag acaaaggtat aaaaacgata
      361 actaataaaa ttgtaattat gccaatcaat gccggaatta tcaaaccttt gagcactctt
      421 gttcccatta tttttccggt gatacctgtc ccgagtgcaa ttccaatagc cgcacaagcc
      481 attacaacac cggttaagga attggaaata tgataatacc ctttacaatg aacaataata
      541 ttcatttgta ccatattcgc aatcaaccaa aaagcagatg taccgaaaac cgcactatta
      601 acaagcttaa atctgtctgc aaacttataa tttgacttta cgaacttata tgggttaata
      661 gttgaactcc cagtcatttc aaccggcaat tcttctgctc tgattttttt tgcaaaataa
      721 aaacctaaga aagcaacacc aattccaatt gatgcaaaca acaaaagaga ataatgatca
      781 gaaatcaatg aagcgataaa agtaccgatc agaattccca taaaagccat tgtttcaaac
      841 ataccactgc cgtaggatac accatcttca ccaccaatat cacgtatcag actatatttt
      901 gagggtgaat aaaggcaact ttgagtacca agagcaaaaa cggaaagtac agccagtaag
      961 acccattgct tataaaaagc gacacatgcc agcagcataa ccggaacttc aatcagcttt
     1021 aacaacctta acacctttaa tttggaatac tttacagcca ggctgcctcc aatgggagaa
     1081 aaaatgagat atggtacaac gagacaagat gcaaccaaag atattagctg tgactgagat
     1141 agccatgtcg gtaatgacca ggtaacagaa atgaaaataa tcgaattttt cagaaaatta
     1201 tcattgaaaa cacccaaaaa attagttaca aataattctc tccacttttt attgttacct
     1261 ttcattgcca tccttttaca ggcaaaatta ccctttttta tccgcgaaac agctatctat
     1321 tgaaataaat taataaaaac aggttaaatg ttaaacaaaa cgaggtttta actgttttta
     1381 tagataagat aaattgtaag aaaacgtaac aacttttatt acttttgatt aaccataatt
     1441 tctcaatttc tttagtcaat ttttttttga gttaatttat gttaattgtt aatttaacat
     1501 ttaattttaa cttaactttg taaagtatat gaagaagagt actatttggg ttttaatgat
     1561 aatgatgacg ataactttca gtgcgctcat ccttgttcag gcaaaatttc tgaaggcaac
     1621 ttcggaaatg gctgaaaaac aatttgatga agcagtgaaa cgaagtatga ttcagactgc
     1681 aaatttactt gaagagaatg aggcactaga atatcttgca cagacgctgg atgataatga
     1741 atatgtgcaa tctgaaagca aatcgaataa ttttgtcatc aatggtcaaa aagacacatt
     1801 gaaatctaac tggaaaagtt catcgtcaat aagtccacga atacgtctat ctaccggaca
     1861 cggcaaggct actattgaag aaacatcaaa ataccttcaa caaaaatttc agtcaaattt
     1921 ctcccgatca aagacaatac tagatcaggc agtattcaga tggcttcgcg aaaacgaaaa
     1981 caaagaaatc agcgagcgag ttgattttac cgatctcaat gaaacgcttg aaacatatct
     2041 taagaacaac aatgtagatt taccgttcta ttatacaatt gtagataata gaaataaaat
     2101 aatctatcag tgcaacaaaa caattaacac caatctaaga ccaaataccg atatatatgt
     2161 acaggagctg ttccctcatg aggacagtta tcgcaaagca tatatgcagg tagtttttcc
     2221 aactaagcaa agttttatct acgaatcgct caatctgttg ctgccctctg tgggtgtgat
     2281 cgtttttact tttatcattt ttgttttgac catagtcatt atcttcagac aaaagcaact
     2341 gaacaacatg aagaatgatt tcgttaataa tatgacacat gagtttaaaa ctccgatttc
     2401 aactatatca ctagcgtcac agatgttaca ggatccggga gtaggcaaaa atccggatat
     2461 gatgaaacat gtaaccaatg tgataaggga tgaaacaaaa cggctgagtt tacaggtaga
     2521 aaaagtactt cagatgtcca tgttcgagaa agaaaaatcg acactcaaat tcactgagat
     2581 acacataaac aacctgatta aggatattat tggtagtttt tcgctgaaag tcaccagcaa
     2641 agggggaaaa atcaattcaa gtctgaaagc ccaatccgat attgcttatg ttgacgaaat
     2701 acatttcacc aatataattt tcaatctgat ggacaacgct ctcaaatata gcgacaagcc
     2761 tctgatactc aatcttgaaa cctggaatga aaaagacaac ctgttgatta gcgttgaaga
     2821 caatggaatc ggaatgaagc gggaggattt gaaacgtata ttcgataaat tctatcgtgt
     2881 ttcaacaggc aatttgcata atgttaaagg gttcggacta ggacttgctt acgtaaaaaa
     2941 aatggtcaca gaccataatg gaagcataaa agttgaaagt gaacccggaa ttggaacaaa
     3001 attcacaata ataataccaa cacttaaaaa aaacaattat gaatgacgaa aaagtaaaaa
     3061 ttttgctgtg cgaagatgat gaaaatcttg gcatgctgct cagagaatac cttcaaacaa
     3121 aaggtttcta ctgtgactta caaccagatg gcgaagctgg ttataaatct ttttcccgca
     3181 ataaattcga tttgtgcgta ttggatgtaa tgatgcctaa aaaagacgga tttgctttgg
     3241 cagcagatat ccgggccatg aatacggaag tacctattat cttcctcact gcaaaatcaa
     3301 tgaaagaaga tattctgcaa ggcttcaaac ttggtgctga cgactatctg tcaaaaccat
     3361 tcagtatgga agaattacta tatcgtattg aatctattct gcgccgtgta cgtggcaaaa
     3421 aatccaaaga tgtagttacc tatcaaatcg gaggtttcac attcgacgct cagaaacaaa
     3481 cgcttgagtt tgaaggagaa atcaaaaaac tgactactaa agaatctgaa ctcctcaacc
     3541 tgctcgctgc taatgccaac aatattctgg aacgtaattt tgctctcaaa acaatttggg
     3601 tggacgataa ttacttcaat gcacgcagta tggacgtata tataactaaa ctgcgtaaat
     3661 tactgaaaga cgaccagaat gtagcaatta tcaatatcca tggtaaaggc tataaactta
     3721 tcatgcctca ggtaaaagaa gattaatcgg ggaaatggtt acattataaa aaatgcgatg
     3781 ctaaaacatc gcatttttta tttgaatttg ttttcaatgt cttctgtttg tatcatcgaa
     3841 agaattaatt tgccatcggg ggtatacata tggttttcgc aggtaaacaa gttatcgaac
     3901 aactccctca tctcagttaa attcagggtt tgtccatact gtattgctgt gtgtcgagtt
     3961 atctgaatgg caatttcttc ctttattgat tctttcactg taatttcggt ttcacttaca
     4021 gttgcaatca tatctttcaa taattgtaac gtggaagtca tttcaatctc tgaaggaatt
     4081 ccattgatag cgtattcact ttttccaata ggaactatat caaaacctac ccagctcaaa
     4141 tcaggcagta tttgttccat tacaccgaat tcatctttgc tgagttccag aatttcggga
     4201 aacagaactt gctgtgtaat tcctttttga ctttccgttt tcttcataaa acggtcaaac
     4261 aggactcttt tatgagctct gtgctgatcg attatcatca aacccgactt gacggtggta
     4321 agtatatatt tatttttaaa ctggtaaaat tccattgaat taacagcaag atcaagcatc
     4381 ggctgttccg gttctaatcg cgtctcgaaa tgatcatctg cttccggttg aactgactgt
     4441 tgttcgggtt gatatatttc ttttttgaat tctccgtaca attgttccca atccaattgc
     4501 tggcgttttg gctgagaact taatctgaaa ggattgtaac tcggatcgaa atgtgttcct
     4561 ggaggtctga tagcagtatg cgagtcaagt acaggtattt caggtgaatc atcctgatca
     4621 aaatcgagtg gaggcattac ctgaaatttg ccaagtgctt ccttaacgga tgccaggagt
     4681 attgaccaga ttgcctgctc attttcgaac tttatctccg tttttgaagg atgtacattt
     4741 acatcgattg attgtgtgtc tatatcaaaa taaatgaaat aattcggatt ttcattaggc
     4801 ttaatcaact gattatatgc aaccattaca gctttatgaa aatacggatg acgcatatat
     4861 cgtccattca caaagaaata ctgattagct aatttctgag catattcggg tcgtccgaca
     4921 aaacctgtga ctgacaaaat acttgtattt gtctcaatag gcagtagctg ttgttcaagt
     4981 tttttcttcg aaacaccgtt gaacacatgt tcaattcggg ctttcagtga ggtggctgga
     5041 agggacatca gttccagagt tccatcatac aatgagaaac taatatcggg atgaaccagt
     5101 acgatccggt aaaattcatt acgtatatgt gtcatctcgg ttgaaggaga tttcagaaac
     5161 cgtcttcggg caggaacatt gaagaacaaa cttttcacag ctatgttagt gcccttgttg
     5221 cattgtacgg attcctggcg aaaaacgcgt gtacctgcta tttcaatcag tgttccaagc
     5281 tcttccgttt ccttacgtgt acgtaactcc acttgtgcca cagcagctat tgatgccaat
     5341 gcttcacccc taaatcccat tgttcgaagt gtaaacaaat cggctgcgtc ttttattttt
     5401 gatgttgcat gccgttcgaa cgacatacgg gcatctgttt cactcatccc cactccatca
     5461 tcaacaatct gaatcaaagt acttccaccg tcttttatca gtacttgaat attttttgct
     5521 ccagcatcga tagagttttc caccaattct tttataactg atgccggccg ctgaattacc
     5581 tcacctgcag caatctgatt agctactgaa tctggtaata aatgtattat atctgacatt
     5641 ctttttactt acatgacaag attttctttc atgaacagaa actggcatgt ttaattta
//
LOCUS       DAOPYB010000195         5682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_17737_length_5682_cov_8.372845, whole genome shotgun sequence.
ACCESSION   DAOPYB010000195 DAOPYB010000000
VERSION     DAOPYB010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17737_length_5682_cov_8.372845"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1346
                     /locus_tag="PKH58_11570"
     CDS             105..1346
                     /locus_tag="PKH58_11570"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNX89714.1"
                     /translation="MNFIDLFAGAGGLSEGFIRAGYKPVAHVEFDTAACFTLKTRQAY
                     YYLKQDNKIDTYIDYLKGSISRDELYSYIPVEILSSVINKTIGSDNSNIFKEIDKQLS
                     NHDVDFIIGGPPCQAYSLVGRARDEYNMEHDKRNYLYIHYNKFLKKYNPKFFVFENVP
                     GLLSAKNGQYYKNMKNLFDKSGYHIELYHLEANNFGVLQNRKRLLIIGYKKELNCNLP
                     DFEKYRCDLSYTVKNIFSDLPKLQAGEGIEKFVSYSGKTNTWLVSSEVRNGIKVLTQH
                     VARPHTNQDKEIYRIAVDKWNTKKERLDYNDLPERLKTHQNRHSFFDRFKVVAADMPY
                     SHTVVAHIAKDGHYYIHPDIEQNRSISVREAARLQSFPDDFFFEGVKEGQTRTAAFKQ
                     IGNAVPSLMAYYIAEILKGLA"
     gene            1347..>1537
                     /locus_tag="PKH58_11575"
     CDS             1347..>1537
                     /locus_tag="PKH58_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX89715.1"
                     /translation="MIDYSKYKTSLANPEASSMIETLRAIGYSLESAIADIIDNSISA
                     AAKNIWVDYDWKGPGTMIS"
     assembly_gap    1538..1637
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1638..2992
                     /locus_tag="PKH58_11580"
     CDS             <1638..2992
                     /locus_tag="PKH58_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX89716.1"
                     /translation="KNIWVDYDWKGPGTMISIIDDGTGMNNEELINAMRPGSRHPLEV
                     REEKDLGRFGLGLKTASFSQCRKFSVISKPAEGKISYWCWDIDYVTQSKSWSLINYCP
                     DNKFETEIEKLNSGTAVIWWEIDRVTKDTSADSSESVDKFMKQMENVKIHLSMVFHRF
                     INDGLNIYFRGRKISSWDPFMIGFDGLQAKPEMKIGNGKITLKGYVLPHRSKLTAEQY
                     EYGKGPKESWTAHQGFYVYRNKRLLVAGDWLGFFKKEVHYDLCRIFVDIPNDVDDDWQ
                     IDIKKSIARPPLRFREQIYSLAKDVRAQALEVYRHKGRVIRRRFSNEEFHQFWEERKR
                     HGKRFYKINRDHPMLRDLFGKANSLKSDIEKVIQFIEETVPVPLITLQENENISPHGQ
                     PFEGIDQEPVRNTMIAMFSNLVKQGKSADEAKAVILNIEPFNFFPEYLEYLNDIDNAE
                     "
     gene            2982..>5682
                     /locus_tag="PKH58_11585"
     CDS             2982..>5682
                     /locus_tag="PKH58_11585"
                     /inference="COORDINATES: protein motif:HMM:NF022063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HNX89717.1"
                     /translation="MLNNAILTIRTLLPESGSVTREEIEKQVDFVLTNPRFKDLDRNL
                     LIREIETLYRIRIDEFRVIEDHERKRPWVLQKKANLADNFWKRYKTYLQYNKNFAPET
                     IDHLDRLTDKTLDGLYDPSLEIGFDKKGLVVGQVQSGKTSNYTGLVCKAADAGYNMII
                     ILAGLHNNLRTQTQLRIDEGFLGFDTKYQRAFNTGNSRIGAGIGHVIYPVHSFTSSDE
                     NGDFSKRGTYNFHTREPIIAVVKKNKTRLENLYRWLSVHASDDGQGNKKIKDKTLLLI
                     DDEADNASINVSNDPEKRSAINKAITQILALFAKSGYVGYTATPFANIFIPLNENDIF
                     PRDFIINLPAPSNYIGPEKIFGFRLVEDDDPSETVLPIVFRINDYADFVPDKHKAADP
                     LPSFIPESLKCAVRCFILTCAVRRLRGQQNEHNSMLVHVTRYIRWQNHIYEMVSNLFN
                     YYRRGIDQNMPEIIEEFRKTFEVDSANYSSYKTITEQIRSSAFSQIDARLIVHSWEDV
                     LNELNNTVSKIVIREVNGGSGDILNYFDHKEGLSVIAVGGDKLSRGLTLEGLSVSYYL
                     RASKMYDTLMQMGRWFGYRPGYVDLCRLFTSRELNEWFCHITHASEELREEFDYMSDV
                     ADSTPEKYALKVRTHPGVLQITASNKMKSTVDIQVSWSGRLVESYELKKDINTIKNNL
                     ETSVRFFNMLDNNYQSADRFFLWKNVHSDHILDFFKGFQILDNLKAYDPVNLTRFINL
                     KLSDGELTSWRVALMSKPKASFQHPVNINNLTKKIGCYIRTQDEKNSGSDIYYLRKSH
                     IISPKDEFIDLTADEYKKAEELTEEQWRKKKKEGSPSYPNGEIVRNEIRHPENPLLLI
                     YLLDPSGADTPNYNIPYVGFAVSFPGSRNNSFVSYRINENLIDW"
BASE COUNT         1822 a         1005 c         1114 g         1641 t
ORIGIN      
        1 ggaaaatctg aaactgcttg aggatgatgg gtggcatgta gtcactattt atgaatgtga
       61 attaaaaaat gaaaaaatta ctaaaacttt aaaccattta ttgcatgaac ttcattgatc
      121 tttttgctgg tgctggtggt ttatctgaag gttttatccg cgcgggttat aagcctgttg
      181 ctcatgttga gtttgacaca gcagcctgct ttacactaaa aacaagacag gcatattatt
      241 accttaaaca agataataaa atagatactt atattgatta cctcaaagga agtatttcca
      301 gagacgaact ttactcttat attcctgttg aaattctttc atctgtaata aataaaacca
      361 ttggctccga taactcaaat atatttaaag aaattgataa acaactcagt aatcacgatg
      421 ttgatttcat tattggtggc ccgccttgcc aggcatattc tcttgtaggt cgggcccggg
      481 atgaatataa tatggaacac gacaaacgaa actaccttta cattcattac aataagtttt
      541 taaaaaaata caatccgaaa ttttttgttt ttgaaaatgt gcctggcctc ctttctgcaa
      601 aaaacggcca gtactataaa aatatgaaaa atctgttcga taaatccggt tatcatattg
      661 agctgtatca tttggaagca aataattttg gtgttctgca gaacagaaag cgattactga
      721 ttattggtta caaaaaagag ctgaattgca acctgcctga ttttgaaaaa taccgttgtg
      781 atctatccta tacagtaaaa aacatttttt ctgatcttcc aaaattgcag gcaggtgaag
      841 gtattgaaaa gtttgtttca tattcaggga aaacaaatac ctggttggta agttcagaag
      901 tcagaaatgg aattaaggta ttaacgcaac acgtggcaag gcctcataca aatcaggata
      961 aagaaatata tcggattgct gttgacaagt ggaatacaaa aaaggaacgt ctcgattata
     1021 atgatctccc tgaaagatta aaaacacatc agaaccggca ttcttttttc gatcgtttta
     1081 aggttgttgc agccgatatg ccatattccc atactgttgt tgcacatatt gcaaaagatg
     1141 gtcattatta tattcacccg gacattgagc agaatcgttc tatctcggtc agagaagcag
     1201 cgcgtttgca atcatttccc gatgattttt tctttgaagg ggtaaaagaa ggacaaacac
     1261 gaactgcagc ctttaaacag attggaaatg cagtaccttc attaatggca tattatatag
     1321 ctgaaatctt aaagggatta gcataaatga ttgattattc aaaatataaa acctcactag
     1381 ccaaccccga agcttcatcc atgattgaaa ctttgagggc tattggctat agccttgaat
     1441 cagcgattgc tgatataatc gacaattcaa tttctgctgc cgcaaaaaat atctgggttg
     1501 attacgactg gaaaggtccc ggcactatga tttcaatnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnncaa aaaatatctg ggttgattac gactggaaag gtcccggcac
     1681 tatgatttca atcattgatg atggaacagg gatgaataat gaggaactta taaatgcaat
     1741 gcgtccgggc agcagacatc ctctggaagt cagagaagaa aaggatcttg gccgcttcgg
     1801 gcttggtctt aaaaccgctt ctttttctca gtgtcgaaaa ttttctgtta tttcaaagcc
     1861 tgctgaaggt aagatttctt attggtgttg ggatattgat tatgttacac aatcaaaatc
     1921 ctggtcgctg ataaactatt gcccggataa taaatttgaa accgaaattg aaaaactcaa
     1981 ttccggtaca gccgttatct ggtgggaaat agacagggtt acaaaagaca catctgccga
     2041 tagtagtgaa tctgtggata aatttatgaa acagatggaa aatgtaaaaa ttcatctgtc
     2101 aatggttttc caccggttta ttaatgacgg cctgaatatt tatttcagag gaagaaaaat
     2161 atcttcctgg gaccctttta tgattggctt tgatggatta caggcaaaac cggaaatgaa
     2221 aataggcaat ggtaaaataa ctctcaaagg ttacgtattg cctcatcgtt caaagctcac
     2281 agctgaacaa tacgaatatg gcaaaggacc gaaagaaagc tggacagctc accagggctt
     2341 ttatgtttac cgcaataaaa gattgcttgt ggcaggcgac tggcttggtt tctttaaaaa
     2401 agaggtgcat tatgatctgt gcagaatatt tgttgacatt ccgaatgatg tagatgatga
     2461 ttggcagatt gatataaaaa aatcaatagc aaggcctccg ttacgtttca gggaacagat
     2521 ttattcactt gcaaaagacg ttagggcgca ggctctggaa gtttataggc ataagggaag
     2581 ggtaataaga agacggttct caaacgaaga atttcatcag ttctgggagg aaaggaaacg
     2641 tcacggtaaa cggttttata aaataaacag ggatcatccc atgctgcgtg atttattcgg
     2701 taaagcaaat tcactaaaat ctgacatcga aaaagttatt cagtttattg aagaaacagt
     2761 ccccgttcca ttaatcacgc ttcaggaaaa tgaaaacatt tctcctcacg gccagccctt
     2821 tgaaggtatt gaccaggaac cagtaagaaa tacgatgata gcgatgttct ctaatctggt
     2881 aaagcagggg aaatccgcag atgaagcaaa agctgttatt cttaatattg aaccatttaa
     2941 cttttttccg gagtatctcg aatatttaaa cgatattgat aatgctgaat aatgccatac
     3001 ttacaataag gaccttgctt cctgaatccg gttcagtcac cagggaagaa attgaaaagc
     3061 aggtggactt tgtattgacc aaccccaggt tcaaagacct tgacagaaat cttttaataa
     3121 gagaaataga aacactatac aggatacgca ttgatgagtt tcgtgtcatt gaggatcacg
     3181 aaagaaaacg tccatgggtg ttacagaaaa aagctaacct tgctgataat ttctggaaac
     3241 gatataaaac ctacctgcag tacaataaaa attttgctcc cgaaactatc gatcacctgg
     3301 atcggcttac cgacaaaacc ctggatgggc tttatgaccc gtcacttgaa atcggttttg
     3361 acaaaaaagg ccttgttgta ggtcaggtac aatcaggtaa aacatctaac tatacaggtt
     3421 tggtttgtaa agccgcagac gccggttata atatgataat tatcctcgct gggcttcaca
     3481 acaatctcag aacacagacc caacttcgaa ttgatgaagg ttttcttggt tttgacacaa
     3541 aataccagag agcttttaat acaggtaaca gcagaattgg cgccggtatt ggtcatgtta
     3601 tatatccggt tcatagtttc acatccagtg atgaaaatgg tgatttcagc aaacggggca
     3661 cttacaattt tcacacccgg gaaccaataa ttgcagttgt taaaaagaat aaaacaagac
     3721 ttgaaaattt gtaccggtgg ctgtctgtgc atgcttctga tgatgggcag ggaaataaaa
     3781 agataaaaga taaaacatta ttacttattg atgacgaagc agataatgct tcaataaatg
     3841 tctcaaatga tccggaaaaa agaagtgcaa ttaataaggc aattacgcaa attttagcgc
     3901 tttttgcaaa atcaggttat gtcggatata cggcaacacc ttttgccaac atcttcattc
     3961 cgctgaacga aaacgatatt tttccgcgag actttatcat taatcttccc gcaccatcta
     4021 actatattgg cccggaaaag attttcgggt tcaggctcgt tgaggacgat gatccatcag
     4081 aaacggtgtt accgattgtt ttcagaatta atgattacgc tgattttgta cccgacaaac
     4141 acaaagctgc cgatccttta ccctcattta ttcctgaatc attaaaatgt gctgttcgct
     4201 gttttatcct gacttgtgca gtcaggagat tacggggcca gcagaatgag cacaactcaa
     4261 tgctggtaca tgtaacccgt tatataagat ggcagaatca tatttatgaa atggtctcaa
     4321 atcttttcaa ttattacagg cgtggtattg atcagaacat gccggaaatt attgaggagt
     4381 tcagaaaaac atttgaggta gattcagcaa actatagctc ctataaaacc ataacagaac
     4441 agataaggtc atctgctttt tctcaaattg atgcccgcct gatagttcat tcctgggaag
     4501 atgttctcaa cgaacttaac aacacagttt caaaaattgt tatacgtgaa gttaacggtg
     4561 gttccggcga cattttgaat tattttgacc ataaagaagg attatccgta attgcagttg
     4621 gtggcgataa actctcacgt ggccttacgc ttgaaggtct ttctgtaagt tattatctgc
     4681 gtgcttctaa aatgtatgac accctgatgc agatgggcag atggtttggt tatcgccccg
     4741 gatatgttga tttatgtcgt ctttttacca gccgtgaact taatgaatgg ttctgtcata
     4801 ttacgcatgc atcagaagaa ttaagagagg agtttgatta catgtctgat gttgctgatt
     4861 ccacgcctga aaaatatgca ttaaaggttc gtactcaccc gggtgtattg cagatcacag
     4921 catccaacaa aatgaaatca actgttgata tacaggtttc atggtcaggc cgtctggtag
     4981 aatcctatga gttaaaaaag gatattaata caattaaaaa caatcttgaa accagtgtcc
     5041 gttttttcaa tatgctggat aataattatc aaagtgcgga cagatttttt ctctggaaaa
     5101 atgtacattc tgatcatatt cttgactttt ttaaaggttt tcagattttg gataatctca
     5161 aagcttatga tcccgtaaat cttactcgtt ttattaatct taaattatca gatggtgagc
     5221 ttaccagttg gcgcgtggct ctcatgtcca aacccaaagc atcctttcag catccggtaa
     5281 atatcaataa cctgacgaaa aaaatcggat gttacatccg tacacaggat gaaaaaaact
     5341 caggttctga tatttattac ttgcggaaat cgcatattat cagtcccaaa gatgaattta
     5401 tagatcttac ggctgatgaa tataaaaaag cagaagaatt gacggaagaa cagtggcgta
     5461 aaaagaaaaa agagggtagc ccttcttatc cgaacgggga aattgtccgt aatgaaatca
     5521 ggcaccccga aaatccctta ttgctgatat accttctcga tccttccggc gctgatacac
     5581 caaattataa tattccttat gttggttttg ctgtcagctt tcctggtagc agaaacaata
     5641 gttttgtatc ataccgtatc aatgaaaatc ttattgactg gt
//
LOCUS       DAOPYB010000196         5655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_17864_length_5655_cov_10.797500, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000196 DAOPYB010000000
VERSION     DAOPYB010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17864_length_5655_cov_10.797500"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..636)
                     /locus_tag="PKH58_11590"
     CDS             complement(337..636)
                     /locus_tag="PKH58_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89718.1"
                     /translation="MGLRKPPFVFTEQGVAMLSSVLNSDSAILVNIQIMRVFTKMRAL
                     LDSHKEILRKLELIEKKDIEQDEKILLVFEYLKQLEQAKQEETIIKERKRIGFKP"
     gene            complement(909..1286)
                     /locus_tag="PKH58_11595"
     CDS             complement(909..1286)
                     /locus_tag="PKH58_11595"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNX89719.1"
                     /translation="MKKKILLVASILFGLMFINSGLNKFFNYMPMPKDVPENMMKVMT
                     AFMEVGWLMPLVAVAEIAGGLLFITNKYRALGAIIIFPVMVGIILTNIITAPSGLPLA
                     LVLFAIEIWVIIENREKYLPMIK"
     gene            complement(1623..1991)
                     /locus_tag="PKH58_11600"
     CDS             complement(1623..1991)
                     /locus_tag="PKH58_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX89720.1"
                     /translation="MDKPAILYVDDEIINLKLLEANLEKKYHVLLAPSGAHGLEMLES
                     RNDVQVVISDMKMPGMSGLEFILKAREKFPSLYYYLMTGFDVSADIQEALSRGQIVTC
                     FTKPVSMGVIDTEIKKVIRE"
     gene            complement(1997..3085)
                     /locus_tag="PKH58_11605"
     CDS             complement(1997..3085)
                     /locus_tag="PKH58_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX89721.1"
                     /translation="MLKIKFEYRITLAYLVIGSLWILFSDRLVDNFIDDKSLMTDMMT
                     YKGWFYVMVTAALFFCFLKRHLYKLRKAEAELERHRNHLQELVVEKTRNLDLALKELN
                     TTNLELSEKNDIINDQNAELKDALQHLKNTQSQLLQAEKMASLGILTAGVAHEINNPL
                     NYILGGYTGLQNYFSENGIANERVSMLLENIKTGIDRSAAIVSGLNQFSRSRDKYDER
                     CEIHSIIENCLVILNHQLKDRVEVIKDFTNEPFLLTGNVGKLHQVFLNILVNASQAID
                     ERGRITIKTEKSGKWLHIDVSDTGSGISKENLPKITDPFFTTKDPGKGTGLGLSITFT
                     IIREHKGSLEFESELNKGTTVRIKLPIN"
     gene            complement(3659..4111)
                     /locus_tag="PKH58_11610"
     CDS             complement(3659..4111)
                     /locus_tag="PKH58_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89722.1"
                     /translation="MENYENNQPEDKPINDENEIKTYLLETSKWGKFLAIVGYVGIAI
                     LAILALCVVIGFTTRGIRTNEDSRMRFFGILYLVIAFVYYFPVNFLYKFAIQMREGLE
                     SDNKQSVTSGFQNLKSLFRFIGIVTIVILSIYAFLLIIVLPLSLLVAK"
     gene            complement(4460..5206)
                     /gene="fabG"
                     /locus_tag="PKH58_11615"
     CDS             complement(4460..5206)
                     /gene="fabG"
                     /locus_tag="PKH58_11615"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNX89723.1"
                     /translation="MKLLEGKTALITGASRGIGKSIALAFANEGASVAFTDLRRDELM
                     EQTENELKALGVKAIGYASDASSFEDSERVVDLIAAEFTKIDILVNNAGITRDNLLMR
                     MPETDWDLVLKINLKSAFNLTKAVQKYMLKQRSGSIINMSSVVGVKGNAGQANYSASK
                     AGLIGFTKSMAQELGSRNIRCNAVAPGFIETDMTAKLSDEVRKEWAQKIPLRRGGKPE
                     DVAKACIFLASELSDYVTGQVISVCGGMVM"
BASE COUNT         1477 a         1172 c         1184 g         1822 t
ORIGIN      
        1 aatctttaat tacttcgaac attgcaccat cgaattactt cgcttggcgt taacggtttg
       61 gcggtatgtt ttgttggtga gccccgacct tagtagggta cggcgcgctt tttgccgtta
      121 tggtacaaac ttggtgaatt aacaaagtgt tgcggtatgt agtttataag tgattaattt
      181 tttctcatgg tatagaaaac tggtaagtga acgatggcgc ggttcaggtt tatcattagt
      241 tacgaatcca ggtaaaaggc tcagagtcaa acaaaaagca tgacgaactt atttacttta
      301 ttgtccgtgt taggcttaga tttttatatt ttacaatcac ggtttaaatc ctatacgttt
      361 tctttctttg ataattgtct cttcttgttt tgcttgttca agttgtttga gatattcaaa
      421 taccaagagg attttctcgt cctgctcaat gtctttcttt tcgattaatt caagttttct
      481 tagtatttct ttgtgagaat caagcagtgc tctcatctta gtaaatactc tcattatttg
      541 gatgtttact aaaatggcac tatcactatt aaggacactt gaaagcattg ctactccctg
      601 ttctgtgaat acaaaaggag gttttcttaa gcccattcga tcactattgg atatcacaat
      661 ttgtgatttc caattttagc attaatttaa acttattgcc agtctcccta atgaacagga
      721 atcaagaaat ctctctatga ggcagggagc taaaaattta agcctaacgt tttcgcatat
      781 ggccagtggc agagttcagg gcacaaactt gtctgggtac actgggatgc ctgcagagca
      841 cagacaatag catttgtata gcccgccatt ggcaatattc ggtgttggcg tgttgttgtt
      901 ttaatacttt attttatcat tggtaaatat ttttcacgat tttcgataat tacccaaatt
      961 tctattgcga acaacacaag agccaatgga agtcctgatg gcgctgttat tatgttagtt
     1021 aaaattatcc ccaccataac cgggaaaata attattgcgc caagagccct gtatttattg
     1081 gtgatgaaaa gtaatcctcc tgcaatttct gcaacagcaa caagtggcat tagccagcca
     1141 acttccatga aggctgtcat aactttcatc atattttccg gcacatcttt aggcattggc
     1201 atataattaa agaatttatt gagtcctgaa ttaataaaca tcagtccaaa aaggatactt
     1261 gctaccaata gaattttctt tttcatatct tttggtttta atggttaatt aatgcgtttt
     1321 ttaaagttgt caaaattgtt catgtgattt tttacaatgc acgccaatta gtaaatatca
     1381 tcataaactt accacttttt agtctctgat caaaagattt catcggctcc tggccagtat
     1441 ggtaatgccc gcgatgcctt ctcccgcccg aacagacagc cgggattgat aagccgatcc
     1501 gtttcagccg tccggcgacc atcctgacgt tggcggccgg ccactaatcc atcaatataa
     1561 caggactcaa aactcaaaga gctccaattt atagtttcag ggttccgggc ccagcgatcc
     1621 gattattcgc ggatcacttt tttaatctcc gtatcaataa ctcccatact gaccggcttt
     1681 gtgaagcagg taacgatctg gccccgggac agggcttcct gtatatcggc agagacatca
     1741 aaccccgtca taaggtaata ataaagggat ggaaattttt ccctggcttt cagaataaac
     1801 tcaaggccgc tcattccggg cattttcata tcgctgatga caacctggac atcgttacgc
     1861 gattccagca tttcgaggcc atgcgccccg cttggggcca gcagcacatg atattttttt
     1921 tcaagatttg cctctaaaag cttgagattg ataatttcat catccacata cagaattgcc
     1981 ggcttatcca tgagtattaa tttattggaa gtttaattct gaccgttgtt cctttgttca
     2041 gctccgactc aaattcaagg cttcccttat gttccctgat aatggtaaaa gtaatcgaca
     2101 gaccaagccc ggtccctttt ccggggtctt tggttgtaaa gaagggatcg gtaattttcg
     2161 gcagattttc tttgcttatg cccgatccgg tatcagaaac atcaatatgc agccattttc
     2221 ccgatttctc agttttgatg gttatgcgtc cgcgttcgtc gatggcctgg ctggcattca
     2281 ccagaatatt aagaaagacc tggtgtagtt taccgacatt tccggtgagc agaaaaggct
     2341 cgtttgtaaa atctttaata acttcaaccc tgtctttcaa ttgatggttc agtattacca
     2401 ggcagttttc gatgatggaa tgaatctcgc accgttcatc atatttatcc ctgctcctgc
     2461 tgaactggtt cagaccggat acaatagccg ccgacctgtc gatacccgtt ttgatgtttt
     2521 ccagcagcat gctcaccctt tcattcgcga taccgttttc gctgaaataa ttctgaaggc
     2581 ctgtatatcc gcccagaata taatttaacg gattattgat ttcatgggcg acgccggccg
     2641 tcaggatgcc cagcgaagcc attttctcag cctgcagcaa ctgcgattgg gtattcttca
     2701 gatgttgaag ggcatctttc aactctgcgt tctggtcatt gatgatgtcg tttttttcgg
     2761 atagttccag gttggtggta tttaattctt tcaatgccag atcaaggttt cttgtttttt
     2821 caacaaccaa ttcctgcaga tggtttctgt gcctttccag ttccgcttcg gctttcctta
     2881 atttgtacag atgccgcttc agaaaacaaa aaaacagcgc tgccgtgacc atgacgtaaa
     2941 accacccttt atatgtcatc atatccgtca taaggctctt atcatcaata aaattatcga
     3001 ccagccggtc ggaaaaaagt atccacaggc ttccgataac cagataggca agggtgatgc
     3061 gatattcaaa cttaattttt aacatcgttt ttctgcttaa tcgccgtgaa cctttgaaga
     3121 agttgagacc tttccttccg gacaaatctt ctttgtataa agcttattat ttaaaacaaa
     3181 gataatcatt gaagaaacat ttacaacgat atcggcccgg acttttggtt aatggttcaa
     3241 tatcacctgg caattttcgg ggacagagac taaatttcgt tcctgtgaat ttataattta
     3301 taaatgtctg attttgttat gtttaagatt atgatgaaca agaatatttt taattctgag
     3361 aatagataca ggtttatatc tccaggtaac aggttactgt gtttgtcccg gaacttgaaa
     3421 aggttttccc tgaaccggag catgaaagac gcacctctgg atttataaaa aaagcctgaa
     3481 tccgtttccc ctatgcctgt catactgcaa taaataccat tattccggca cttttgcatt
     3541 taagattact gaaagcagaa ggcaagaaca aaaacacgga gattttttcc aaccagaaat
     3601 agttgccgag agtaaactcc atttaaatct tcgtttagta tctgcaagtt aataagcatt
     3661 attttgcgac cagtaaggac aatggcaata cgataatcag gagaaatgca tagattgata
     3721 atatgacaat ggtcacaatt ccgataaacc taaaaagcga tttcaagttc tggaatccgg
     3781 aagtgactga ttgtttatta tctgattcca ggccttccct catttgaatt gcaaatttgt
     3841 ataagaaatt tacaggaaaa taataaacaa atgcgattac cagatatagt attccgaaaa
     3901 atcgcattcg tgaatcttca tttgtcctga tacctctagt ggtaaatcca attactacac
     3961 aaagtgcgag aatagccaga attgcgattc cgacatatcc tacgattgcc aggaatttgc
     4021 cccatttcga ggtttctaac agataagttt taatttcgtt ctcatcatta attggtttat
     4081 cttccggctg attgttctca tagttttcca tgctgttaac tttaaagttt aatatgatta
     4141 gttttagttt tgttaatctg aaaggataat tattatttgt tcaaaacgct gtatgtctgt
     4201 tgtttatgta agtgaaacca tcccattaaa ccgggcaata tttagggtaa atttccttaa
     4261 ggcgtattcc tgatatttat tcttatataa gttatttata tcagctaaat taaaatttat
     4321 ttaaataccc aataataccc ccacttattt tgacggaatt tggtaaaata gacaaaaagc
     4381 ccggaaaaaa tcccgggcct gaaaaaaaat ggtttggggc aagaaaccgg gtcgtaactt
     4441 ccgggtcacg gaccccggtc tacatcacca tgccgccgca cacgctaatc acctggccgg
     4501 tgacgtagtc cgacaattcc gaggcaagga aaatgcatgc tttggcaacg tcttcgggtt
     4561 ttccgccacg gcgcaggggg attttctgag cccattcctt gcgcacttca tcggaaagtt
     4621 tggcggtcat atcggtttcg ataaatcccg gcgcaacggc attgcaacgg atgttacgcg
     4681 agccgagttc ctgggccatc gatttggtaa atccgatcag cccggccttg gaagccgagt
     4741 aattggcctg tccggcattg cctttcacgc ccaccaccga actcatattg ataatggagc
     4801 cgctgcgctg tttcagcata tatttctgca cggccttggt gaggttgaag gcgcttttca
     4861 ggttgatctt caaaaccagg tcccagtcgg tttcgggcat gcgcatcagc aggttatcgc
     4921 gggtgatgcc ggcgttgttt accaggatat cgatctttgt aaattcggcg gcgatgagat
     4981 ccaccacgcg ttcactgtct tcgaacgagc ttgcgtcgga agcgtacccg atggctttca
     5041 cgcccagcgc ttttaattcg ttctccgttt gttccatcag ctcatcgcgg cgaagatcgg
     5101 tgaatgcaac gctggcccct tcgttagcaa atgccagggc aatggacttt cctatcccgc
     5161 gcgatgcgcc ggtgataaga gcagtttttc cttcgagaag tttcatatat tgttaagttg
     5221 gattaattgg cagaaatacg ggaagaggga gtggcgtgtg tggattgagg ggcttttggc
     5281 tgttggccgt tagctgttgg cttttagcgt tttgagtttt gcgttctgtg ttttgtgttt
     5341 cgtgtttcgt gttttgcatt tcgttgttcg ggggcagggt ttcagcgttt cagcgtttca
     5401 gtgtttcagc gtttcagtgt ttcagtcctg cctgcattgc gcaaggctgt gggagggggc
     5461 agggtttcag cgttttgtgt tttgcgtttt gtgtttcgtg atgcgtgatg cgttattaag
     5521 gtttcgtgga attcgcgatt gctttcgtgg aattcgtggt tcgtgatgcg ttgttcgtaa
     5581 ttcgtgatta gtgatttgtg gaatgtctta tcatgatttt ggttgacaaa aaagaccaaa
     5641 cttctacttc aattg
//
LOCUS       DAOPYB010000197         5616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_18046_length_5616_cov_12.898220, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000197 DAOPYB010000000
VERSION     DAOPYB010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18046_length_5616_cov_12.898220"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PKH58_11620"
     CDS             complement(<1..1046)
                     /locus_tag="PKH58_11620"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNX89724.1"
                     /translation="MSDLKKHNSEIQLFSDELFVGISQIIEKARTNVAIFLNSETTLM
                     YWSIGHYTNTNLKVNNRTQYGAKILATLSQLLTSQFGKGYSYSALTRMCKVATVFDNE
                     NIATLSQQLSWSHLIELATIPDDTKRNFYTQLSIFQKWDVRELRSKADAMLFERTAIA
                     TKPEDVLTQALTKLQDEKILSPELVFKSSYILDFLDLPADYSEKELESALVKNIENFL
                     MEMGSGFAFLERQKRISIDSTDYHLDLLFYHRKLKRLVAIDLKLGKFKPEHKAQMELY
                     LRWLQKYEMQAGEETPIGLLLCSEGNSEHIELLMLDQNDIKVAQYLTELPSKQWFADK
                     LHRALEIAKQNTFN"
     gene            complement(1039..3681)
                     /locus_tag="PKH58_11625"
     CDS             complement(1039..3681)
                     /locus_tag="PKH58_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNX89725.1"
                     /translation="MATNKLSLSRLATFLEEACESLRGNMDASEYKEYIIAMLFLKRV
                     NDQFDVHRDTRKKNLIEKRHLSAPEAIYGELERQNAPEYDFFVPLAARWRKLEGDPDV
                     NEQGEKINYIKDLQEEIGDHLNKALAALEDANLDKLEGVLKSINFNRSIGKNNKQITD
                     EDLRELIREFNKKNLTDDNLEFPDLMGAAYEYLIKFFADSAGKKAGEFYTPNEVVRLL
                     ANILEPEADAEIYDPTVGSGGMLIECKNYVESRYGSAKNLSLYGQEKSGTVWSLSKMN
                     MLFHGIYDSKILNGDTLMNPLHIENGELKTFDIVIANPPFSQNYTRDGMKFKERFNFW
                     MPNKGKADFMFVQHMVASVNNAGRMAVVMPHGVLFRAGDERKYREWLINKGYLEAVIG
                     LPSGLFYGTGIPASVIVINKKDAHLRDKVLFINADREYKEGKNQNKLRPEDIEKIAFV
                     YRNKIEIPKYSKLVGKNVVVDELCNLESEEYNLNIRRFVDNAPPAEPQDVHAHLQGGI
                     PESEVKALADAFASYPGLSDKLFTELKKDYLKFRTDGINGISSKEDIKILFNESNEVK
                     ATLDAYTQGLNNWWNLIVADFEALPEKKNVFELYRKFSESFTEALKVLPFGEDLGGAV
                     LDNYQSRGSLAAYWSELNTDLKSVAASGWNAELIPDDEILESQYPEVLKELRENEARR
                     DELQALFTEVNELDDDVWQEEEYEVWRSKELKEQKELIKSINVELKEAQKEIKLLKQR
                     IKAGAVGLEKQLVELETVLAPSLLERAGGEADRITRHTALEDELKQCKKTIKEIKDRK
                     QQLVDLARMSITPDEAKELILKRWKLILHQTINGYLQNHSRQLLQAVENFYDKYTIPL
                     HGILTEREKETKLLNDFLVKLGYE"
     gene            complement(4086..5297)
                     /locus_tag="PKH58_11630"
     CDS             complement(4086..5297)
                     /locus_tag="PKH58_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX89726.1"
                     /translation="MKAAYIKRPAYMDKIRPFIGKPIIKVLTGQRRVGKSYILLQLMD
                     EIRLMHPDANIIYINIEYNEYEPLRTHSALYQYVSERLMENVPNSLLVDEIQEVQSFE
                     LCLRSLMAESKCDIICTGSNAQMLSGELATTLSGRYIQFQVYSLSYEEFLMFHQLNDS
                     NESMISYLRLGGMPYLIHIGLETDIVMEYLRNVNSTILLKDVVARENIRNVSFLENLT
                     AYLADNTGNLLSAFNISKYLKSQHQQIPTQTILNYLRALTQAYYVHKVQRADVRGLKI
                     FEIGEKYYFEDTGLRNVLRRFDFRNDVQKVMENVVYLHLIRNGYRVFVGTNGNREIDF
                     MAEKDDERLYVQVAYMLADEQTLQREFGNLMAIDDHYPKYVVTMDEITAGTAYKGIRQ
                     IHLRDFLKNYH"
BASE COUNT         1435 a         1256 c         1045 g         1880 t
ORIGIN      
        1 tgattaaatg tgttttgttt tgcaatttcc aaggcacggt gcagtttatc ggcaaaccat
       61 tgtttgcttg gcagttcggt aaggtattgc gccactttaa tatcgttttg gtcgagcata
      121 agcagttcta tgtgttcgct gtttccttcg ctgcaaagca acaagccaat aggtgtttct
      181 tcgcccgctt gcatttcgta tttttgcagc caacggaggt atagttccat ttgtgcttta
      241 tgttcgggct taaatttacc gagtttcagg tctatggcaa ctaatcgttt cagtttgcgg
      301 tggtaaaaca agaggtcgag gtggtagtcg gtactatcaa tcgatatacg tttttggcgt
      361 tcgagaaaag cgaatccgct gcccatttcc atcaaaaagt tttctatgtt ttttaccaat
      421 gcgctttcaa gttctttttc gctataatcg gctggcaagt ccagaaaatc gagaatatac
      481 gaacttttaa acaccaattc gggcgacaga attttttcgt cttgcagttt tgtcaacgcc
      541 tgtgttaaaa catcttccgg ttttgtagca atggcagtgc gttcaaacag catcgcatcg
      601 gctttactgc gaagttcgcg cacatcccat ttttggaaaa tgcttagttg tgtgtagaag
      661 ttgcgtttag tatcatccgg aatggttgca agctcaatca ggtggctcca actcaattgt
      721 tgcgacagtg tcgcaatatt ctcattgtca aaaacagttg ccaccttaca catacgtgtc
      781 agtgcagaat atgaatatcc ctttccaaat tgcgaagtta atagttgcga cagtgtcgca
      841 agaattttgg ctccgtattg tgtacggtta ttcactttca aattcgtatt ggtgtaatgc
      901 ccaatactcc aatacattaa tgtcgtttcg ctatttagga aaatggcaac attagtgcgt
      961 gctttttcaa taatttgcga aattccaaca aatagctcat cgctaaacaa ttgtatctcc
     1021 gaattgtgtt ttttcaaatc actcatatcc caacttcact aaaaaatcat ttaacaattt
     1081 ggtttccttc tctctttcgg tcaaaatacc atgcaaagga atagtgtatt tatcataaaa
     1141 attttcaacg gcttgcaata gctgacggct gtgattttgc agatagccgt taatggtctg
     1201 gtgtaagatt agtttccagc gttttaatat tagttctttg gcttcgtcgg gagttatact
     1261 catacgggca agatcaacta attgctgttt gcggtcttta atctctttaa tggtcttttt
     1321 gcattgtttc agttcgtctt ccagtgcagt gtggcgggtt atccggtcag cctcaccccc
     1381 cgccctctcc aaaagagagg gtgctaagac cgtttccaat tcaactagtt gtttttctaa
     1441 acctactgcc cctgctttta tgcgttgttt cagcaattta atttcttttt gcgcttcctt
     1501 gagttctaca ttgattgact taatcaattc tttctgttct ttcagttctt tactgcgcca
     1561 tacttcatac tcttcttcct gccacacatc atcatccagc tcatttactt cggtaaacag
     1621 ggcctggagt tcatctctgc gggcttcgtt ctcacggagt tctttcaaca cttcggggta
     1681 ttggctctcg agtatctcat cgtcgggtat cagttcagca ttccagccac tcgctgccac
     1741 acttttcagg tcggtgttta attcactcca ataggcagcc aatgaaccac ggctttggta
     1801 attgtcaagt actgccccac ctaaatcctc accaaaaggg aggactttaa gagcctcggt
     1861 aaaactttcg gagaatttac ggtagagttc aaaaacattc tttttttcgg gcagagcctc
     1921 aaagtcagca acaattaaat tccaccagtt atttagacct tgcgtgtaag catccaaagt
     1981 agcttttact tcgttacttt cattgaacag tattttaata tcctctttgg atgagatgcc
     2041 atttataccg tctgtacgga atttaaggta atcctttttt aattcggtga agagcttgtc
     2101 acttaagccg ggataacttg caaatgcatc agctaaggct ttcacttcgc tttcgggaat
     2161 gcctccctgc aggtgagcat gaacgtcctg cggctcggca ggtggggcat tgtccacaaa
     2221 cctgcggata ttgaggttgt attcttcgct ttctaaattg cacagttcat caacgacaac
     2281 gtttttgccc accagcttgc tgtattttgg tatctctatt ttattccgat acacgaaagc
     2341 aattttctca atgtcttcgg ggcggagctt gttttgattt tttccttcct tgtattcgcg
     2401 gtcggcattg ataaagagta ctttgtcgcg tagatgagca tcttttttgt ttatcacaat
     2461 cacacttgcc ggaatgcctg tgccataaaa caaccctgaa ggcaaaccaa ttacagcttc
     2521 cagataccct ttattgatta accactcacg gtatttgcgt tcgtcaccgg cacggaataa
     2581 aacaccgtgt ggcattacca ctgccattcg tccggcattg ttgacactgg ctaccatgtg
     2641 ctgaacaaac atgaaatcgg ctttgccctt gtttggcatc caaaagttga agcgttcttt
     2701 aaacttcatg ccgtcccggg tgtagttttg cgaaaatgga gggttggcaa ttacaatgtc
     2761 gaaggttttc aattcaccat tctcaatgtg caaagggttc atcagggtat cgccattcaa
     2821 tatcttgctg tcgtagatac catgaaaaag catgttcatt ttgctcagac tccaaaccgt
     2881 tccactcttt tcctgtccgt aaagcgatag atttttagct gaaccgtaac gactctcgac
     2941 atagttcttg cattcaataa gcataccacc acttccaact gttgggtcat aaatttcagc
     3001 atctgcttcg ggttcaagga tattggcaag gagacgcacc acttcatttg gagtgtagaa
     3061 ttctcctgct ttttttcctg cactatcggc aaaaaattta atcaggtatt cgtaagctgc
     3121 acccatcagg tccgggaact ccaaattatc gtcggtcagg ttctttttat tgaactccct
     3181 gattaactcc cttaaatctt catcggtaat ttgtttgtta tttttaccta tgctgcgatt
     3241 gaagttgatt gacttcaaaa cgccttccag cttatcaagg ttggcatctt ccagggctgc
     3301 aagcgctttg ttgaggtggt ctccgatttc ttcctgcaag tccttaatgt aattgatttt
     3361 ttcaccctgt tcatttacat cagggtcacc ttccaacttt cgccaacgag ctgcaagcgg
     3421 tacaaagaaa tcatattcag gagcattctg tcgttctaat tcaccataaa tagcttcagg
     3481 tgcagaaagg tgacgttttt caatcaggtt tttttttctg gtatcgcggt gaacatcaaa
     3541 ctggtcgttt acccgcttca gaaacaacat ggcaatgata tattccttgt attccgaagc
     3601 atccatgttg cctcttaaac tttcacatgc ttcttcgaga aaagtagcta aacgtgaaag
     3661 tgataattta tttgtagcca ttatactagt ctaaactctt ttattatgta atttgtattt
     3721 ttatcgtatg tttttctaaa ccaaaataga atttctataa tgccggttgt aattttatgg
     3781 aatacccgga aatcaaattt ccatattaat tctcattttt ttggttatga gtttactcca
     3841 tggaataata aatatataca cttattcaga ttatgaaaac ccgggttgac attgtagcca
     3901 tttttttaac ccccaaactt actaattttc attcacattt ccgcgattgt taaaaatatt
     3961 attctcccgc gttgcggtga attttataac ggttcatatt cctcgttcac gaataatcag
     4021 atacccaatt ccacattcta cattctacat tctacattct tctttcttct ttcttctttc
     4081 ttcatttaat gataattttt cagaaaatca cgcagatgta tctgccggat acctttgtaa
     4141 gcggttccgg ctgtaatctc atccatcgtg actacatatt tgggataatg atcatcaatg
     4201 gccatcaggt tgccaaattc acgttgaagg gtttgttcat cagccagcat atatgccacc
     4261 tgaacataca gtcgttcatc atccttttcg gccataaaat caatctccct gttaccgttg
     4321 gtgcctacaa aaacacggta tccgtttcgg atcaggtgca ggtaaaccac attttccatc
     4381 accttttgca catcgttccg gaaatcgaaa cgccggagaa cattcctcaa tccggtatct
     4441 tcaaaatagt atttttcacc gatttcaaag attttcagtc ccctgacatc ggcacgctgc
     4501 actttatgaa catagtaagc ctgagtcaat gcccgcaggt aattcagaat agtctgtgtt
     4561 ggtatctgct gatgttgtga tttcaggtac ttgctgatat taaatgccga taacagattt
     4621 cccgtattgt cggcaagata ggccgtgagg ttttcgagaa aagaaacatt acgaatgttc
     4681 tcccgtgcaa ccacatcctt cagcaaaatg gttgaattta cattccggag gtattccatc
     4741 acaatatcgg tttcaagacc aatatgaatc aggtatggca ttccgcccaa cctgagatat
     4801 gatatcatac tttcattgct gtcgttcaat tgatgaaaca tcaaaaattc ttcatagctg
     4861 agcgaataaa cctgaaactg tatatacctg cccgagagtg tggtagccag ttcgcccgaa
     4921 agcatctgag cgttgctgcc ggtacagatg atatcacact ttgattctgc cattaatgat
     4981 ctcagacaca gttcgaatga ctgcacttcc tgtatttcgt caacgagcag agaattaggc
     5041 acattctcca ttaaccgctc tgaaacatac tgatatagag ccgagtgggt gcgcagtggc
     5101 tcatactcgt tatattcaat attaatgtaa ataatgtttg catcgggatg cattagcctt
     5161 atttcgtcca tcaactggag caaaatataa cttttaccca cacgtcgctg acctgtaagt
     5221 actttgatga ttggcttacc gataaatggt ctgattttat ccatataagc cggacgtttg
     5281 atgtaggctg ccttcatgtt ttcttctgtt ataattgaaa tattcgcaaa tatataaaaa
     5341 caattcaatt ataattgaat atttgatgct tttttctgtt ttcttccggt atgttttaac
     5401 ttctgcccgg tgtaactgtg ctccttttca ttaaaactaa aatgtgcagt cctccataaa
     5461 cccatcacat cagctctatt tcactccgtc accaggtctc caggtcacca aatctctaag
     5521 tctccctgtc tccaggcccc cttcacccgg tctccccgtc ctttattctt tccttttctt
     5581 attaatttgt gatgctattc gccgtataaa actgtc
//
LOCUS       DAOPYB010000198         5587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_18177_length_5587_cov_8.848879, whole genome shotgun sequence.
ACCESSION   DAOPYB010000198 DAOPYB010000000
VERSION     DAOPYB010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18177_length_5587_cov_8.848879"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            431..3121
                     /locus_tag="PKH58_11635"
     CDS             431..3121
                     /locus_tag="PKH58_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNX89727.1"
                     /translation="MAPNRKRNWIIVVSAIVLVFIILLILPLVFRGKITEIARRELNK
                     QLTAEVNFDKLKVSFIRNFPDASVSLKNIRVTGTGDFAKDTLLFCRDVNLVINLKSLF
                     GNTGYDVKKLEFTDSKVLAHVLKDGRANWDIMKTDTTAALDTSAMSFKFKLKNVIINR
                     ADVRYADDEGDIAADVKNLNLRMNGDLTADSTLLVTKLTADTLNFWSEGFQYAHNMTV
                     DIDADINADLNSERYAFANNQMKLNAVPFALNGWVQLIDSGYDMDLTLDASKVDFKSI
                     LSLVPAIYAKSFDQLKAEGKVNMSGFIKGKMVGEDYPAFDLNLAVADAWFQYPGLPKS
                     VKDIQLISRLTNPGGSLDKTVIDFPKLSFNLGGNPFAAQGHIATPLTDPDLTMKANGK
                     IDLGMIKEVYPLEKETTLNGILTMDVNMAGKMSYFENNMFERYRFAGTVNVQNMLLKM
                     KDLPQDVAISQALLTFSDRYLNLSNLKMKIGKNDLSADGKAENYMAYALRDKTLKGNF
                     TVKSTYFNLNDFMAAGTDTKNDSSSLTVVKIPKNLDLSLDGSFSQLIYDKMNFRNAAA
                     ALKVADGSLNIQKMNVDAFGGVMALTGTYSTSDPGKPFVDFDLDMKEISFKEVFQQVT
                     VMKKIAPIFDKAFGKFNSRLKINSLLQQDMMPVLSSILGNGSLNAKAVAVKEVNALTM
                     LGEALKSNELANLTLKDLALMFEIKDGKVNTKPFTIKAANMEMKLGGSTGLDQSIDYA
                     GNIRLPEKMNLGKFSNVGFKILGTFTKPKIQLDLKNTLTTLAEEKKAVVMQKADSLKN
                     VAIDKGRAEREKALKQAQAKADQILEKAKLAGDKLIVQAQAQGDSLVAKASNPVMKQL
                     ARKGADQLMKEARKQAENLNKKAKDEADKVMQQANEATGF"
     gene            3170..4582
                     /locus_tag="PKH58_11640"
     CDS             3170..4582
                     /locus_tag="PKH58_11640"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX89728.1"
                     /translation="MKHITKIIIALMLLTSASLRAQTPQEADTLFKTNRYSQAMEVYK
                     KLLKAKPKNDLYNFRIAQCAYELKDFEQAITYFQKTSDKYPLKNYYLGEIFFDNYRFD
                     ESVQAFMTYLEKFNPEDTQITTAQRKLKQAELGAKFLKRIEDVEIVDSMVVDKKDFLN
                     KIPVSTELGSLKQMRLKLPGGSETDKIEYITQRGDRTWFSDTIGGQSDLFTSYKLLDR
                     WAPAEKQITINTPDNENYPFLMLDGVTLCFASDGEGSLGGYDIFITRFNTDANKFLPA
                     ENVGMPFNSPFNDYMMIVDELRKVGWFVSDRYQPRGKVAVYQFIPNVEKKIIRSENPD
                     TLIRFARAEVFSKSAKDKRIKSGYVAGKQISQPENRIIVCDSVVYSNAADFRSPDARE
                     AYLQLQTLQSEYDAAKSELEGLRETYARSSDDNVRTSTGENILNLEAKLREMQPKIDA
                     LTLKWRNDEIKFLQNSLDKK"
     gene            4619..5092
                     /locus_tag="PKH58_11645"
     CDS             4619..5092
                     /locus_tag="PKH58_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89729.1"
                     /translation="MEITIVVILLVLGIVFLLLEIFMFPGISVAGVAGAVFTAAAVWY
                     AYTHIGAVAGNVTLIGGVVLLGVAVWMFMRSRALDKMSLKTSITGTVEAIDELKISVG
                     DKGTTISRLAPMGKIKINGTVVEAKTDNEFIDPNTEVIIKEVYKTNVLVGRGDDV"
BASE COUNT         1712 a         1204 c         1234 g         1437 t
ORIGIN      
        1 ctcattactg attactgatt actgattact gattactgat tactgattac tgattactga
       61 ttacctaccg ttaatcattc atcattctat cagtttcctg gttctgattg cctcaccaga
      121 ttccgcgctg cgtttcttcc ctgtaaccat ttttgcacag tttttgaagg acttccattt
      181 attctttaat taatcaccgg taaacattta gaatattcct gtaagtcatt ataaaacgtg
      241 acttgtcata atgtcacctc tacggggctt taatttttag catcagtagg tgcgacaatc
      301 cggtagtaaa cagattacac ccaacaacac aagctcagaa agagcgtcat tattttttct
      361 tttgttttta tcggacaaca atatattttt atctattttt gtaaaattaa tcaccgtaaa
      421 cctctatatt atggctccta atcgtaaacg taactggatt attgttgtat ccgctattgt
      481 tctggtgttt attatcctgt tgatactgcc attggttttc aggggtaaaa ttaccgaaat
      541 agcccgccgc gaactgaaca aacaactgac agccgaggtt aatttcgata agctgaaagt
      601 cagttttatc cgaaatttcc ccgatgcatc ggtgagtctg aaaaacatcc gtgtcaccgg
      661 aaccggcgac ttcgcgaaag atacactgct cttttgccgc gatgtcaatc tggtcattaa
      721 tctgaaaagt ctctttggca ataccggtta tgatgtgaaa aagctcgaat tcaccgacag
      781 taaagtgctg gctcatgtgc tgaaggatgg acgggctaac tgggacatca tgaaaaccga
      841 tacaactgcc gctttggata ccagtgccat gagtttcaaa ttcaaactta aaaatgtgat
      901 catcaaccgg gctgatgtgc gctatgccga cgacgaggga gatatcgccg ccgatgtaaa
      961 aaatctcaac ctgcgcatga atggcgacct gacagccgat agtaccctcc tcgttaccaa
     1021 acttactgcc gacacgctca acttctggag cgaaggtttc cagtacgccc acaatatgac
     1081 agtggacatt gatgcggata tcaatgccga cctcaactcg gaacgttatg cctttgccaa
     1141 caatcagatg aagctgaacg cggtaccttt tgcacttaat ggctgggtgc agctcatcga
     1201 cagcggttac gacatggatc tgaccctcga tgcttcgaaa gtggatttca aatccatcct
     1261 gtcgctggtg cctgccatct atgccaaatc gttcgaccag ctgaaagccg aaggaaaagt
     1321 aaatatgagc ggtttcatca agggaaaaat ggtgggtgaa gattatcccg ccttcgacct
     1381 caaccttgct gttgccgatg cctggttcca gtatccgggc ttgccaaaat cggtgaagga
     1441 catacagctg atcagccggt tgacaaaccc cggtggttcg cttgataaga ccgtgattga
     1501 tttcccgaaa ctttccttta acctcggagg caaccccttc gcggcacagg gacacatcgc
     1561 cacaccgctg accgatccgg atctgacaat gaaggctaac ggtaaaattg acctgggtat
     1621 gataaaagag gtgtaccccc tcgaaaaaga aaccacgctc aacggaatac tgacgatgga
     1681 tgtaaatatg gccggaaaaa tgtcgtattt tgagaataat atgttcgaaa gataccgatt
     1741 cgcaggtaca gtcaatgtgc agaatatgct gcttaagatg aaggatttac cgcaggatgt
     1801 tgccatcagt caggcactgc tcacattcag cgaccgatat ctcaatctca gtaacctgaa
     1861 aatgaaaatc ggtaaaaacg atctgagtgc tgacgggaaa gctgaaaact acatggcgta
     1921 tgccctgcgt gacaaaacac tgaaagggaa tttcacggtg aaatcaacct atttcaatct
     1981 caatgatttt atggccgccg ggaccgatac aaaaaatgac agttcgtcgc ttaccgttgt
     2041 gaaaataccc aaaaacctgg atttgtcact cgacggttcg ttcagccaac tgatctacga
     2101 caaaatgaat ttccgcaatg ccgcggctgc attgaaagtc gccgatggca gtctcaacat
     2161 tcagaaaatg aatgtggacg cgtttggagg tgtcatggcg ctgacaggaa cctacagcac
     2221 ttccgatccc gggaaaccct tcgtggattt tgacctggac atgaaggaaa tatcctttaa
     2281 agaagtgttt caacaggtta cggtcatgaa gaaaattgct cccatcttcg acaaggcttt
     2341 cgggaaattt aattccagac tgaaaattaa ttcgctgctg caacaggata tgatgcctgt
     2401 actttcctcc atacttggta atggttcgct caacgccaaa gcggtggctg tgaaggaagt
     2461 gaacgccctc acaatgcttg gtgaagcgct gaaaagcaac gaactggcca acctgaccct
     2521 caaagacctc gcgctgatgt tcgaaataaa ggatggaaag gtaaatacaa aacctttcac
     2581 catcaaagcg gcaaatatgg aaatgaaact gggcggaagc accggactgg atcagtccat
     2641 cgattatgcc ggaaatatca gattacctga aaaaatgaac cttgggaaat tttcaaacgt
     2701 tggttttaaa atactcggaa catttacaaa accaaaaatc cagctcgatc tgaaaaatac
     2761 cctgaccaca ttggccgaag agaaaaaagc tgttgtgatg caaaaagctg attccctgaa
     2821 aaatgttgca atagataaag gacgcgctga acgtgaaaaa gcgcttaaac aagcgcaggc
     2881 taaagcagac cagatactcg aaaaagcaaa actggcaggt gataaactga ttgtacaggc
     2941 acaggcacaa ggcgactcgc tagtcgcgaa agcttccaac ccggtaatga aacagctggc
     3001 gcggaaagga gctgatcaac tgatgaaaga agcccgaaaa caggctgaaa acctgaacaa
     3061 aaaggcaaaa gacgaagccg acaaggtgat gcaacaggca aacgaagcca ccggtttctg
     3121 aatattctga cagtaaaaat gtttaacacg gatatttatc atcgtaaaaa tgaaacatat
     3181 aactaaaata atcatcgcgc tgatgctgct cacatcggca tcactccggg cacaaactcc
     3241 acaggaagca gataccctct tcaaaaccaa caggtacagt caggctatgg aagtctataa
     3301 aaaactgctg aaagccaaac cgaaaaatga tttgtacaat ttccgtatag cgcagtgtgc
     3361 ctacgaactg aaggacttcg aacaggcaat aacttatttc cagaagacaa gcgataaata
     3421 tccgttaaaa aattattacc tgggtgagat tttcttcgac aactaccgct tcgacgagtc
     3481 ggtgcaggcg tttatgacat accttgagaa attcaatccg gaagatacac agattacaac
     3541 tgctcagcgc aaactgaagc aagctgaact gggagccaaa tttctgaaaa ggattgaaga
     3601 tgtggaaatt gtggatagta tggtggtcga taaaaaggat ttcctgaaca aaattcctgt
     3661 cagtacggag ctgggaagcc tgaaacaaat gcgcttaaaa cttcccggtg gttcggaaac
     3721 cgacaaaata gaatacatta cccagcgtgg cgaccgcacc tggttttccg atacgatagg
     3781 cggacagtcc gatcttttta catcctataa attgcttgac cggtgggcgc ctgccgaaaa
     3841 acaaattacg atcaacacac ccgacaatga aaattatcct ttcctgatgc tcgatggcgt
     3901 tacgctctgc tttgcttccg acggtgaagg ctcactcgga ggatacgaca ttttcattac
     3961 ccgtttcaat acagacgcaa acaaatttct tccggctgaa aacgtgggaa tgcctttcaa
     4021 ctcgcctttc aacgactaca tgatgattgt tgatgaattg cgcaaggtgg gctggtttgt
     4081 ttccgaccgc tatcagcccc ggggaaaggt ggcggtgtat cagtttattc ccaatgtcga
     4141 aaagaaaatt atccgttcgg aaaatcccga tacgcttata cgttttgccc gtgcagaggt
     4201 attcagtaaa tccgcgaaag ataaacggat aaaatcaggt tacgtcgccg gtaaacagat
     4261 cagtcagcct gaaaacagga tcattgtctg tgattcggtg gtttattcca atgccgcgga
     4321 ttttcgaagt cccgatgcca gagaggcgta tctgcaatta caaactttac agtcggaata
     4381 tgacgcggca aaatcggaac ttgaaggttt acgcgaaaca tacgcgcgat cgtctgacga
     4441 caatgtgagg acatccaccg gagaaaatat actgaacctg gaagcgaaac ttcgcgaaat
     4501 gcagccaaaa attgatgcgc tgaccctgaa atggcgcaat gacgaaataa aattccttca
     4561 aaacagtttg gataaaaagt aaataagtat taactttaag cccaaataga aagaatttat
     4621 ggaaatcact atcgttgtca ttcttcttgt cctcggaatt gtattccttt tgctcgaaat
     4681 atttatgttt ccgggtatat ccgttgcggg tgttgccgga gctgtattta cggctgccgc
     4741 ggtttggtat gcatatacac acattggcgc tgtagccgga aatgtcacac tcattggcgg
     4801 ggtggtgctg cttggcgttg ccgtgtggat gtttatgcga tcacgggcac ttgataaaat
     4861 gtcgctcaag accagcatca caggaaccgt ggaagccatt gatgagttga aaatcagtgt
     4921 aggcgacaaa ggaacaacga tttcgagact ggctcccatg ggtaagatca aaatcaacgg
     4981 aacagtggtt gaagccaaaa ccgacaacga atttatcgat cctaatacgg aagtgataat
     5041 aaaggaggtt tataaaacga atgtactggt gggaagaggg gatgacgttt aacgtttgat
     5101 gtgtagaacc aggactgtga gactttgaga cggtgagagt ggagaatgtg acaatgtgtc
     5161 gatttgccaa tttgaagatg tgctaatttg aaaatttgaa gattgatttt tgagtaatta
     5221 gtgattgctt cgacaatgct tagcacaagt tgtgattagt gaaaagttca tcaggtttcc
     5281 cgaaacaagt acgggacacg catcaagagt aatacagttt atagtcaacg cttaaacaat
     5341 taaacgatta aacgattaaa cgattacaca ctcaacatta cacgcttcaa caattcataa
     5401 ttcatcactc attattcata agtcatatca tgtttctttt tttctttttc ttgtaacttt
     5461 ttattaatat ataaacccgg agattttctt ttctccccct ttagggggaa ggggggcttc
     5521 tctattaact ttaaaacatg gctctcatcg aagtaatttt attaatcgta cttgctgtac
     5581 tgatttt
//
LOCUS       DAOPYB010000199         5585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_18190_length_5585_cov_7.006329, whole genome shotgun sequence.
ACCESSION   DAOPYB010000199 DAOPYB010000000
VERSION     DAOPYB010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18190_length_5585_cov_7.006329"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..3406
                     /locus_tag="PKH58_11650"
     CDS             137..3406
                     /locus_tag="PKH58_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX89730.1"
                     /translation="MGKLKFFLTCLLMASISLVSAQTKTASGTVISAEDGLPVIGASV
                     TVKGNSGIGTLTDLQGRYTISVPSSTNTLVISLVGMKTAEVPATLNQRVTMETGISEL
                     DEVVVVAYGQQRREAVTGAVAQIKTEKIAQRPIASATAALEGQALGVQVNNSYGEPGA
                     EASIRIRGFNSITGSNTPLYVVDGVPMGGNVSDINPNDIESISVLKDASSAALYGNRA
                     ANGVILITTKTGSIGDERLQVVADIKQGVYQRATPDYARLDAKEFMEAQWQSRRNSKF
                     TDAAAGTYNTWADANPGVTNLVKADVGENYNIFNKSWDNLFDANGKMASDAQIKSGYL
                     GDLNWYDGLERTGKRQDYNLNASGGTKKTSYFFSLGYLDEEGFMKFSSGNRFTGRSNI
                     TSMATKWLKVGLNLSASHQRYRSMTATSDDNIAFINPFMFARNIAPIYPVHLHNPETA
                     EYILDANGEKIYDGGSTNGRTQYANRHIIWEYELNNDQTIRNTAETGTFAEISFLKDF
                     KLKVNGNMSNRTSSNSTYDNSTIGDGAGKGRMKQEDYRYKNYTFQQMLTWNKSFNDVH
                     NFDALLGHENYWYNYQYTYIYKQDEKLPNIMELSNFSTNTSIDGYSVDYRTEGYLARL
                     GYNYDSKYFGEFSFRRDGSSRFHPDNRWGNFWSVGGSWVLSREEFIKQYDWINNLKLR
                     GAYGEVGQDGGVGYYAYMGLYESSKNGGKGAYYKSQNDASAITWEKSQSMSIALEGRF
                     FKRLNLSVEYFDKTSKDLLFDVNRPLSIGGTLTSINPVQTMNFGSMSNRGLEIGADFD
                     IIQSKDWTWNIGTNLNLLNNKIKKLPAEFGEEGYESGVRKYMVGRSIYDFYVYAFEGV
                     NKSNGRSLYRLNDKTYYINEPGYEGTGATVGTGEERLPLAAANYTIIDGQAYVYNTSY
                     AKKDYFDSAIPKAYGSFNTMLRWKSVSLSALLTYQMGGKMIDNSYMSLMSIGTSPSAK
                     HKDLLNSWTPEQAGTGIDVNGIPLLNTTQSSFNNATSSRFIMSSDYLTIKNVTLAYDL
                     PRTAVSRLGLSRIRFSASVDNLAIFTAMQGINPQQSFGGVSYNAYVPARVGIIGVNVQ
                     F"
     gene            3429..4994
                     /locus_tag="PKH58_11655"
     CDS             3429..4994
                     /locus_tag="PKH58_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX89731.1"
                     /translation="MKNIKKIALMMVFASAFAGCSDTYMDTVPTSSASTQTIFKSTSN
                     AKQAVNGIAKLMVSQYGDFGQVFSGEGTIKFLFGEYLGENYSRPVLTGWSTVMNATYL
                     ENTTSVTYNGYPWYYYYQLIGNANSIIASIDKAEGTEQERQFIKAQALTYRAYCYTQL
                     VQFYAYRWADSNNGTLVSKRNNGLVIRTEENMTVTDVPLSSSGDVYKQIYKDLDEAIK
                     LYNASALKRDNVWEPNIDVANAVYARAALNRQDYPTAAAKAAEARAGYPLMSNAEYTS
                     GFSAPNKEWIWGSYGGEDQTLYYYGFHSYMAYDANTSISRSNPNVISKELYDKIPSTD
                     IRKGMFLDGKLYPYSKVNGILTNKADEAAVRATRPTMIASHRISAYHSFKFSIKGSIG
                     VGYINNFRTAEMYLIEAEAKYYQGGAQIQGAQAAMNALIKNSGRDANYSCTATGSALL
                     SEIKTYRAIELWGEGFDWLDKKRYNESLVRRNFNNGGSFADAMAKTLDVNAGNKWTYF
                     TPAREYEYNHALD"
BASE COUNT         1853 a         1032 c         1148 g         1552 t
ORIGIN      
        1 atatccctgt ttttaaccct tgctgttgaa aacagattag gaatttaaaa aataatcgat
       61 atttttgcat attatgtttt atatttattt aatacaatca caaataaaaa aaaatgttaa
      121 acaaaaggtt aaatttatgg gaaaactaaa attctttttg acttgcctcc tgatggcaag
      181 tatcagtttg gttagcgctc aaacaaaaac cgcatccggc acggtcattt cggctgagga
      241 cggacttccg gtaataggag cgagtgttac agtaaaggga aattcaggta ttggtacgct
      301 gacagatctt cagggaaggt acacaatttc tgttccatct tctaccaaca cactggtgat
      361 ttcgctggta ggtatgaaaa ctgcagaagt gcctgctacg cttaatcaac gggttacgat
      421 ggagaccggc atttcggaat tggacgaagt ggtagtggtg gcttacggac agcagcgcag
      481 agaagcagtt accggagctg tagcacaaat taaaaccgag aaaatcgctc aacgcccaat
      541 tgcatctgca acagcagctt tggaaggtca ggcactcggt gttcaggtaa acaattcgta
      601 tggcgaaccc ggcgcggaag cctcaattcg tatcagggga tttaattcaa ttacaggttc
      661 caacacccca ctctatgttg ttgacggggt tcctatggga ggaaatgtat cagatattaa
      721 tccaaacgac attgaatcaa tttctgtatt gaaagatgct tcttctgctg ccttatatgg
      781 taaccgtgca gccaatggag ttatattgat tactacaaaa accggatcta ttggagatga
      841 aaggctacag gtagttgcag acattaagca gggagtttac caacgagcta cccctgatta
      901 tgcacgcctc gatgcaaaag aattcatgga agcccaatgg cagtcacgtc gtaattcaaa
      961 gtttaccgat gcagctgcag gtacgtacaa tacttgggct gacgcaaatc cgggcgttac
     1021 taatttagtg aaagccgatg ttggtgaaaa ctacaatatc tttaataaat catgggataa
     1081 cttgtttgat gccaacggga aaatggcaag tgatgcacaa ataaaatctg gttatcttgg
     1141 cgatttgaat tggtatgatg gtttggaacg cacaggtaag agacaggatt ataatttaaa
     1201 tgcatccggt ggaacaaaaa aaacatctta cttcttctca cttggctacc ttgacgaaga
     1261 aggctttatg aaattctcct ccggcaacag atttacaggt cgttcaaaca taacatcgat
     1321 ggcaaccaag tggctcaagg taggtttgaa tttatcagct tcacatcagc gatacagaag
     1381 tatgactgcc accagtgatg acaatattgc tttcataaat ccatttatgt ttgcccgtaa
     1441 tatcgcacct atctatccgg tacatttaca taatccggaa accgctgagt acattctgga
     1501 tgcaaacgga gaaaaaattt atgacggagg cagtacaaac ggacgtaccc agtacgctaa
     1561 ccggcacatc atttgggaat atgaattgaa caatgatcaa acaatccgca atactgccga
     1621 aacaggcacc tttgctgaaa tttcattcct gaaagatttc aaattgaaag tcaatggtaa
     1681 catgagcaat cgcacgtcat ctaacagtac gtacgataac tctaccattg gcgacggagc
     1741 tggtaaggga cgtatgaagc aagaggatta tcggtacaaa aattatacat tccaacagat
     1801 gctaacatgg aataaatcgt tcaacgatgt tcataatttc gatgctttat taggtcacga
     1861 aaattactgg tataactatc aatataccta catttataag caagatgaga agttgcctaa
     1921 tatcatggaa ttatctaact tcagtacaaa cacttcaata gatggatatt cggttgatta
     1981 cagaacggaa ggttaccttg ctcgtctggg atataactac gatagtaagt attttggtga
     2041 attttcattc agacgtgacg gctcatctcg tttccatccg gataacagat ggggtaattt
     2101 ttggtcagtc ggtggtagct gggtgctttc ccgtgaagaa tttatcaaac agtatgactg
     2161 gataaacaac ttaaaacttc gcggtgctta cggtgaagta ggacaagatg gcggtgttgg
     2221 ctattacgca tacatgggac tgtatgaatc aagtaaaaac ggtggtaaag gtgcctatta
     2281 taaatcccag aatgatgctt cagctataac gtgggaaaaa tcacaatcca tgagtatcgc
     2341 cttagagggt agattcttta aacgtcttaa tttatctgtt gaatactttg acaaaacatc
     2401 aaaagattta ttgttcgacg ttaacagacc attatctatt ggaggaacgc tgacatccat
     2461 taaccctgtt caaacaatga actttggttc catgtcgaat cgcggattag aaataggagc
     2521 agattttgac atcattcaat ccaaagactg gacctggaat ataggaacaa atctcaacct
     2581 gttaaataat aagattaaaa aattacctgc tgagttcggt gaagaagggt atgaaagcgg
     2641 agtaagaaaa tatatggtag gacgcagtat ctatgacttt tatgtttacg ctttcgaagg
     2701 agttaacaaa agcaatggac gctctttgta ccgcttaaat gacaaaacat attacattaa
     2761 tgaaccaggc tatgagggaa caggagcaac tgttggaacc ggagaagaaa gattgcccct
     2821 ggcagctgca aattacacga taattgatgg tcaggcatat gtttataaca caagttatgc
     2881 aaaaaaagac tattttgaca gtgcaatacc taaagcatac ggttcattca atacgatgtt
     2941 aagatggaag agtgtttcac tttcagcgtt gctaacttat cagatgggtg gtaaaatgat
     3001 tgacaactca tatatgtcat taatgagcat tggcacatca ccatctgcaa aacataaaga
     3061 tctgcttaat tcatggacac ctgaacaagc aggtaccgga atagatgtaa acggtattcc
     3121 actactcaac acaactcaaa gtagttttaa taacgccacc tcctcccgct ttataatgag
     3181 ttcagattat ctgaccatta agaacgtaac gttggcttat gatttgccaa gaactgctgt
     3241 ttccagactt ggattgagca gaatccgctt ttcagcatca gttgataatt tagccatatt
     3301 tacagctatg cagggaatta accctcaaca atcgtttggt ggagtttcat acaatgccta
     3361 tgtgccagca cgagtaggaa tcattggcgt aaatgttcaa ttttaattaa aagaaaggat
     3421 tttattttat gaaaaatatt aagaaaatcg cattaatgat ggtgtttgct tcagcctttg
     3481 ccggctgttc agatacgtat atggatactg taccaacaag ttctgcatca acgcaaacga
     3541 tatttaaatc aacatctaat gcgaaacaag cagttaatgg aattgccaaa ctaatggttt
     3601 ctcaatacgg agattttggg caggtatttt caggagaagg taccattaaa tttttatttg
     3661 gcgaatacct cggggaaaat tatagtcgcc ctgttttgac gggttggagc acagtaatga
     3721 atgctactta tctggaaaat actaccagtg taacttataa tggttatcca tggtattact
     3781 attatcaatt aataggaaat gcaaactcta ttatagccag tattgacaaa gctgaaggaa
     3841 ctgaacaaga gcgccagttt attaaagccc aggcattgac ctatcgcgca tattgctata
     3901 ctcaacttgt acagttttat gcttacagat gggctgatag taataatgga acattggttt
     3961 cgaagcgtaa caacgggctc gtaataagaa ccgaagaaaa catgacagta actgacgttc
     4021 cgctttcttc ttcaggtgat gtttataaac aaatatataa agatcttgac gaagccatca
     4081 aactttacaa tgcaagtgca cttaaaagag ataacgtttg ggaaccaaat atcgatgttg
     4141 ctaacgctgt ttacgcgcgt gcagctctta acagacagga ttatcctact gctgcggcaa
     4201 aagctgccga agcacgtgca ggatatccgc tcatgtctaa tgcagaatac acttccggtt
     4261 ttagtgcacc aaacaaagag tggatttggg gaagctatgg aggcgaagac caaactcttt
     4321 actactatgg attccattca tacatggcct acgatgccaa cacttctatc tcacgctcaa
     4381 atccgaatgt tatcagtaag gaattatatg acaaaattcc ttctacagat attcggaaag
     4441 ggatgttttt ggatggtaaa ttatacccgt atagcaaagt aaacggcatt ttaacaaata
     4501 aagccgatga agcagcagtt cgggcaacac gaccgacgat gattgcttct cacagaattt
     4561 ctgcctatca tagctttaag ttctctatca aaggttctat tggtgtgggt tatataaata
     4621 atttcagaac agcagaaatg tatttaatag aggcagaagc taaatattat cagggaggag
     4681 cacaaatcca gggcgcacaa gcggctatga atgctttgat aaaaaatagc ggcagggatg
     4741 caaattattc atgtacagca acaggaagcg cacttttatc agaaataaaa acttaccgtg
     4801 ctattgaact ctggggagaa ggctttgact ggttagacaa aaaaaggtac aatgaatcgc
     4861 tcgtaagacg taattttaat aatggaggaa gttttgcaga tgcaatggct aaaacactgg
     4921 atgttaatgc cggaaacaaa tggacctatt tcactcctgc cagagaatat gaatataatc
     4981 atgcgctgga ttaattgaaa aaacaataat gcttataaaa tccccttgca tcccggtgcg
     5041 aggggatttt tattttcaca cctccagtct caaaaaaaag tatccatccc aagctaaccg
     5101 tattaagttt caaaattcaa aatccgattt tgcctgaaaa aagtaatgtt tagtttaaat
     5161 caaggcaacc ggtatgtagt ttgtttgact ggtgtagatt tgcataaagg actgaccgcc
     5221 acactcgaaa aaataccgac gctgtaagct ctaataaacc gggaataact actcccgtac
     5281 aataaatcgt aacgaataaa aaaatcggac atagaggaca tcagcactct aagtccgttt
     5341 cttgcgtaat acggctaact tgggatggtt tcccttttgg aaaaatacat ttcagtgaat
     5401 aaattaatat taacaggcta gtctttataa caggtaacag gctgaaattt aataattcca
     5461 ggcaattttt atagcgaaat ccgctaccct ggcattaaaa actaagctta tcatacggtt
     5521 aagtgttaaa ttttcaaatt tctgaaaata attttcgagc tgatataaac ataaaaatgt
     5581 caaat
//
LOCUS       DAOPYB010000200         5559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_18328_length_5559_cov_10.964026, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000200 DAOPYB010000000
VERSION     DAOPYB010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18328_length_5559_cov_10.964026"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..>3652
                     /locus_tag="PKH58_11660"
     CDS             418..>3652
                     /locus_tag="PKH58_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNX89732.1"
                     /translation="MKKYIYLFAIYFSFNINSLTYSQDQGTVVDFNSIPSTGTIMVFA
                     HQDDDAIWMLPWWGKTQKFICAAMPSTPTFKATIDDLQTFLDNNSYGIDYKSNWIHPW
                     PDITQDEYVNYYWKHDNVNYGYLVNDHLSFEEHSFSRTEINKIKAKIEPYIADKSTKR
                     IITHNIWGEYGHTDHKAVSQAVRELAVKYGKDVWMLGSEIINHQFIDVSVPANINYAY
                     GDFDANNPLFIGNRDVFYNHEVWTWIPTNTTGTHKFIEVVEAGVDKSVLLVPGTEVTT
                     TGPVQDKPGAFIFNGADENADYLTLQGNNDTAFTIAMWVRPDIIRAMDISKMSEYPSY
                     VSCNRTFYMQSDGRITARVSDGSTHTVTSTAAMTAGNWSHILMTSDGSTLKLYIDGVL
                     QGSTSLGIVISGYVTPEFIIGQKQEKENNYKGQVYDVRLYDKVLSESSIVALAGTTPP
                     PVYTINATCGGGGKISPMGLVLNEAGDNRTFTITPDTNYSARVIVDGVTVGAVTNYTF
                     NNITSNHTIDVIFTPLPGVALNKPATAQSFTSVMTTPNKANDADGTNKSYWEANYPPQ
                     WWKVDLQGIYNVSSIVVRNYYDLFRYYNYEIWGSLDNKIFTKITEKTNTKFSGESGDT
                     YNLTNTTARFLKVVMTNNSINPSVHITDFRVYGTESTTTHFIDASAGAGGTISPDSTR
                     VFNNGENQTYNITAAANYRVADVKVDGVSTGAVPDNTFTTSYTFSNITANHTIEAVFA
                     PLPGIALNKPATAQSFVGEGTAPGKANDSDRTNSSFWEASPYPQWWQVDLQNFYDISA
                     VLIRNYHENLRSYQYEIWGSLDNVNFTKIAEKKDNLTTTDAGDMYDLTDTTVTARYVK
                     VVMTRNSFNSSVHISDFRVYGTPSNAYHLITATAGTGGNISAAGTAIVPNATDTTYTI
                     TPNANFGVADVKVDGVSVGAVTSYKFSNVTANHTIQATFTPLTGIALNKPVTAQSFTS
                     ATTTPNKANDADGTNSSYWEATPYPQWWQVDLQGIYDINAVFIRNYYETGRYYQYEIW
                     GSTDNKTFTRIAEKKDTSPTTDLGDMYPLTTTARFLKVVMTNNSINPS"
     assembly_gap    3653..3752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3753..4812
                     /locus_tag="PKH58_11665"
     CDS             <3753..4812
                     /locus_tag="PKH58_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNX89733.1"
                     /translation="VGAVTSYKFSNVTANHTIEATFVLLPGIALNKPTACESYEMGGR
                     GPEMANDPDGSNSSYWAANPYPKWWQVDLQGIYDINAVFIRNYYEPGRYYQYEIWGST
                     DNVTFTKISEKKNNNQTTEFGDLYSNLTAKARYIKVLMTFNSANNSVHITDFRVYGTQ
                     GKTLNISSVMLEGLYNGSNTMRQAQDETGAHWPSGIADHITVELHDAANYSTIVYSAA
                     DVPLSTTGNAEITVPENFSGNYYITIKHRNSIETTSAAAVSFAGSTINQSFGNPANVY
                     GGNLGLSIDDHYLIYGGDVNQDGVVDTGDFSSVVNEVFNYSSGYIFTDVNGDGITDSG
                     DFTTLVNNSMNYVATSHP"
     gene            4877..>5559
                     /locus_tag="PKH58_11670"
     CDS             4877..>5559
                     /locus_tag="PKH58_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89734.1"
                     /translation="MKRIKPKVYTVSALLLMFFVIFNVNAVHKIRYAPLATDAFLLSV
                     KNFKQTSNSKLEFDAYLQDTDPTLPFELASMQFGFLINSAIYAGGTLTVTIDNTNSGL
                     NAIQKITASPNAATKTGSQTVIKLASGIVTAGQGTIISTAVPGTLITHFIITSSVPFP
                     SNITPDLIFTSSTSTTPLYATRVAEFINSLSTQLDVNIGTANAIINGNPVLNAAPTAY
                     AVTGSGSYC"
BASE COUNT         1743 a         1139 c         1060 g         1517 t
ORIGIN      
        1 agagatgtag ctcttttttt tatttaaaga atatggatga tttttacgtt tatataatta
       61 attccgaaac atcggatata tttgatttga tcaataccta aacatcaaac agtatttttt
      121 aaaactttcc tctactcctt tgtttgattt ttttcatata aaaaaacagt ttgtctgcaa
      181 aaaaaatatt ttaccaaaac aattacaaac aattaaaaaa cttttcacca gtttgaaatg
      241 ttttaattca aaatacataa cgctatttta actattaaac gttaattttt aatactcttt
      301 tgcgataatt tatcataatg tttaaaataa ctttcgtaca cttaaatgtt aatccaaaat
      361 atctatcgat gattagtctc attaaatccg gttaattaac tacaaaacct ctttgttatg
      421 aaaaaatata tttacttgtt tgccatttat tttagcttca atatcaacag cctgacatat
      481 tcacaggatc agggtacggt ggttgatttc aactccatcc cctcgaccgg aacaataatg
      541 gtatttgcac atcaggacga tgatgcaatc tggatgctgc cctggtgggg caaaactcag
      601 aaatttattt gtgctgccat gccctccact cccactttta aggctaccat cgacgatctt
      661 cagacttttc tggataacaa tagttatggg attgattata aatcaaactg gatacaccca
      721 tggccggata tcactcagga tgagtatgtc aattactact ggaaacacga taatgtaaac
      781 tatggctatt tggtcaatga tcacctcagt ttcgaagagc attcgttttc aagaactgaa
      841 atcaataaaa ttaaagcaaa aattgaacct tatattgccg ataaatctac aaaaaggata
      901 attacacaca atatatgggg tgaatacgga cataccgatc ataaagctgt cagtcaggct
      961 gtaagggagc tggccgttaa atatggaaaa gatgtttgga tgttgggaag tgaaatcatc
     1021 aatcatcaat ttattgatgt atctgtacct gcaaacataa attatgccta tggagatttt
     1081 gatgcaaaca atcccctctt tatcggcaac agagatgttt tttataatca cgaagtctgg
     1141 acatggattc caactaatac taccggaaca cacaagttta ttgaggtagt tgaagcaggt
     1201 gttgacaaat ctgttttatt agttcccgga acggaggtta ctacaaccgg ccctgttcag
     1261 gacaaacccg gtgcttttat ttttaatgga gcagatgaaa atgctgatta tttaactctt
     1321 caggggaata atgataccgc ctttactatt gccatgtggg taagacctga tattatccgc
     1381 gcgatggata tttcaaaaat gtcggaatat ccttcgtatg tttcctgcaa ccgcactttc
     1441 tacatgcaat cagatggaag aattacagca cgggtatcag atggcagcac gcatacggtt
     1501 acttctactg ctgcaatgac agccggcaac tggtcacata ttctcatgac aagtgatgga
     1561 agtactttga aactttatat tgacggtgta ttacaagggt ccacatcgtt gggtatagtt
     1621 ataagtggtt atgttacacc agaatttatt atcggtcaaa aacaagaaaa agaaaacaac
     1681 tacaaaggac aggtttatga tgtccggtta tatgataagg tgctttcaga atcttcaatt
     1741 gttgcacttg ccggcactac tccacctcct gtatacacta tcaatgctac ttgtggcgga
     1801 ggaggcaaaa ttagtccgat gggattagtt ttgaacgaag cgggggataa caggacattc
     1861 acgataacac ccgatacaaa ttattcagca agagttattg tagatggtgt taccgtaggt
     1921 gcggtaacaa actatacctt caacaatatc acatcaaatc acactatcga tgtgatattc
     1981 actccactac ccggggtagc attgaataaa ccagctaccg cacaatcatt tacctcggtg
     2041 atgactactc ccaataaagc gaatgatgct gacggcacca ataaaagcta ttgggaagca
     2101 aattatcctc cgcagtggtg gaaggtagat cttcagggaa tttataacgt ttcatccatc
     2161 gtagtgagaa attattatga tttatttcgt tattacaatt acgaaatatg gggaagtctt
     2221 gacaataaaa tttttacgaa aataacagag aaaacaaaca caaaattttc aggagaaagc
     2281 ggtgacacat ataatctgac aaataccacc gcccgtttcc tgaaagtggt catgacaaac
     2341 aattccatta atccatcggt tcatattacc gatttcaggg tgtatggcac cgaaagcacc
     2401 accactcact ttatcgatgc ctcggcggga gccggtggta ccatttcacc cgacagtacc
     2461 cgtgtattca acaacggtga aaatcagacc tacaacatca cggcggccgc caattatcgc
     2521 gtggccgatg taaaggtgga cggagtttct accggtgcag tgcccgacaa caccttcacc
     2581 acgagttaca cattctcaaa catcaccgcc aaccacacca tcgaggctgt attcgctcca
     2641 cttccgggca ttgctctcaa caaaccggca acggcacagt cctttgtggg cgaaggtaca
     2701 gctcccggaa aggctaacga cagcgacaga accaacagca gtttctggga agcaagcccc
     2761 tatccgcaat ggtggcaggt ggatctgcaa aacttctatg atatctcggc ggtactgata
     2821 aggaattatc acgaaaatct gagatcatac caatacgaaa tatggggaag tctggataat
     2881 gtaaacttta ccaaaatcgc agagaaaaaa gataacctga ccacaaccga tgcaggcgat
     2941 atgtatgatt tgaccgatac aaccgttacc gcacgatatg taaaggtggt gatgaccaga
     3001 aattctttca actcatcggt acatatctcc gatttcaggg tgtatggcac accgagtaat
     3061 gcctatcacc tcataaccgc aacagccggt acaggtggta acatctcagc ggcaggaacc
     3121 gccatagtgc ccaacgcaac cgatacaacc tacaccatta cccccaatgc caatttcggg
     3181 gtggccgatg taaaagtgga cggagtatcg gtgggagctg taaccagtta taaattcagt
     3241 aatgttacag caaatcacac cattcaggct acatttacac ctctaaccgg cattgcactc
     3301 aacaagccgg ttacagctca gtcattcacc tcggcaacga ctacccccaa caaagcaaat
     3361 gatgccgacg gaaccaacag cagctactgg gaagccactc cctatccgca gtggtggcag
     3421 gtggacctgc agggaattta tgacatcaac gctgtattta tccgaaacta ctacgaaacc
     3481 ggacgatatt atcagtacga aatatgggga agcaccgaca ataagacctt taccaggata
     3541 gccgagaaga aggataccag tccgacaact gatttaggtg acatgtatcc gctgaccacc
     3601 accgcccgtt tcctgaaagt ggtcatgaca aacaattcca ttaatccatc ggnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggtgggag ctgtaaccag ttataaattc
     3781 agtaatgtta cagcaaatca caccattgag gctacatttg tactattacc gggtattgcc
     3841 ctgaataaac caacagcctg tgaatcttat gaaatgggtg gcaggggacc tgaaatggct
     3901 aatgatccgg atggatcaaa cagcagttac tgggctgcca atccttatcc aaagtggtgg
     3961 caggtggatc tgcagggaat ttacgatatt aacgcagtat ttatccgaaa ctactacgaa
     4021 cccggaagat attatcagta cgaaatatgg ggaagtaccg acaatgtgac gtttactaaa
     4081 atttcagaga aaaagaataa caatcagaca accgaattcg gtgatttgta ttcaaatctg
     4141 actgcaaaag ccaggtatat taaagttctc atgactttta attcagccaa taattctgta
     4201 catattaccg atttcagagt ttacggaaca cagggcaaaa cactgaatat cagctctgtg
     4261 atgctcgaag gactttacaa tggcagcaat accatgcgac aggcacagga cgaaacgggg
     4321 gctcactggc cgtccggaat tgctgaccac ataacggtag agctgcacga tgctgcaaat
     4381 tattcaacca tcgtttacag tgcagctgat gttccactaa gcaccacggg taatgccgaa
     4441 atcactgttc ctgaaaattt cagtggtaat tattacatta ccattaagca tcgcaacagt
     4501 attgaaacca cctccgctgc ggcagtatca ttcgcaggaa gcacgattaa tcagtcgttt
     4561 ggaaatccgg ctaatgtgta tggtggaaat ctgggacttt caattgatga tcattatctg
     4621 atttatggtg gagatgttaa tcaggatggt gttgtagata ccggagattt cagttcggta
     4681 gtgaacgagg tattcaacta ttcttctgga tacattttca ccgatgtaaa tggggatgga
     4741 attacagatt ctggtgattt cacaactctg gtcaataata gtatgaatta tgtagctact
     4801 tctcacccct aattgaaatt tgtgtgacta ttttttatta aaaacaaact accaataaca
     4861 attaatacta taagttatga aaagaattaa accaaaagtt tataccgtgt cagcgttact
     4921 tttgatgttt tttgtaattt ttaatgtaaa cgcagtacat aaaattcggt atgctcctct
     4981 tgccacagat gcatttttac tgtcagtgaa gaattttaag caaacatcta actcaaaact
     5041 tgaatttgat gcataccttc aagacaccga tccaacatta ccgtttgaat tagcaagtat
     5101 gcaatttgga tttttaataa attctgctat ttatgcagga ggtacattga ctgtaactat
     5161 tgataataca aatagcggac tgaacgcaat tcagaagatt actgcctctc ccaatgcagc
     5221 aacaaaaacc ggttcacaaa ctgttattaa gttagcatct ggaatagtta cagctggaca
     5281 gggcacaata atatcaacgg ctgttccagg tacacttatt acccatttta tcattactag
     5341 ttctgtacca tttccttcaa atataacacc ggatttgatt tttacatcaa gtacatcgac
     5401 tacaccttta tatgcaaccc gtgtagctga atttattaat agtctcagta ctcaattgga
     5461 cgtgaatatt ggtactgcaa atgccattat aaacggcaat cctgttctta atgccgctcc
     5521 aacagcttat gccgttaccg gatcaggttc ttattgtca
//
LOCUS       DAOPYB010000201         5517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_18528_length_5517_cov_14.870560, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000201 DAOPYB010000000
VERSION     DAOPYB010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18528_length_5517_cov_14.870560"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PKH58_11675"
     CDS             <1..904
                     /locus_tag="PKH58_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HNX89735.1"
                     /translation="NENLKANIEISHFGEEVLKGKQTEWKVKNALGKTISQGILSAKD
                     IQIGNCQQLGEITIPLSFIKEAEKMILEVKLGGTEVSNNWDFWVYPEILPQVDTTSVY
                     ICDKMDDKAKSVLNNGGSVLLQLAGKVTQGKDVVQQLVPAFWNTSWFKMRPPHTTGIL
                     INDFHPVFRDFPTDYYSNLQWWELANRAQVMELTDFPKSFQPIVQTIDTWFINRRLAM
                     LFEARVGNGKIIVCSADIQKDPDKRIVARQLRYSILKYMNSNLFLPENELTLEVIENL
                     TIKRGEWLDMSMTKDAPDELKKNI"
     gene            1033..3828
                     /locus_tag="PKH58_11680"
     CDS             1033..3828
                     /locus_tag="PKH58_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNX89736.1"
                     /translation="MKNKSCCLLIIVLFASIHAMASDTNWPPVTTETKPWTRWWWLGS
                     AVDKDNLTYNISELGKAGMGGMEITPIYGVKGNDAKNIDYLSPRWMNMLTHTETEARN
                     SGMKVDMNTGTGWPFGGPEVTLEDAATKAIFQEYNANGGEDVSLKITMNDPKQKKVAY
                     LSRLMAYSDKGKKLDVTNLVSKEGDFKWNVPKGDWKLIALFVGKTLQEVKRSAPGGQG
                     YVMDHFSAKAVKNYLTKFDNAFAREGTSYPNSFFNDSYEVYNADWTPTFLEEFQQRRG
                     YKLEDYFPEFLAKGKTDISCRVVSDYRQTLSEMLLTDFTQQWTAWAHSHGAITRNQAH
                     GSPANIIDLYAAVDIPECEIFGISDFDIPGLRKDEMRKINDGDPVTLKMASSAAHITG
                     KKFTSSETFTWLTEHFRTSLSQCKPEIDQVFTSGVNHVFFHGTPYSPREAAWPGWLFY
                     ASVNMSPTNSIWKDAPAFFSYITRVQSFLQYGEPDNDLLVYFPVYDIWYEQQSDYYYA
                     FGIHGLRDRLPKFYRAVEKIRESGRDVDYITDRYIMSATVENGLIKTIGGTTYKALVL
                     PAVRFMPLETAQKLTQIANDGAKIIFVDHYPEGVPGLKDFNKRQSKLHKLINRFPSGN
                     FSKSKVKKIGKGQIITGKDYDELLTMANIKDESFSKEFKGQYIRRKNETGYHYFFTQL
                     TNNPVNNLVELGVDARSAVIFDPLTGKSGKAQLLRSRRNRPLLCLNLKPGQSMIVKTY
                     TNEDVDVPDWNYYQIIPFRLLKRNEGWKLNFIQSEPAIKDTFDLQGLGSWTELSNPDL
                     KRNMGTGKYSIKFNFKKTGKEYRLTLGDVRESASVYVNGQFVETLFSVPFETTIGKYL
                     NDGENLLEVEVTNLPANRIADYDRRGVDWRIFNEINFVDLNYRKTLYDKWETVPSGLL
                     GPVEIYEITPTKSFMLD"
     gene            3998..>5517
                     /locus_tag="PKH58_11685"
     CDS             3998..>5517
                     /locus_tag="PKH58_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNX89737.1"
                     /translation="MKKLILSLIILVLLTGFTSSEKKIKIFIAGDSTAQTYKSDKDGL
                     IRGWGQMLPLFLDEKVEVVNHAMGGRSTKTFLNEGRWDRLMAQVRKGDYVFIQFGHND
                     ASTRPERYASQADYYDNLVKMIQDVRAKKAHPVLLTSVVIRTFVNGNLTDDRLKAYPV
                     IMRQIAKEYKVQLIDVNQKTRDMVTVLGDKKSVAYYRHTEPGIDPAKPEGVKDDTHMM
                     EKGATQVAYFIAEGIMEQKMKGLSEHVRLGADDKSIQSFMKTTNQYVYLFSYFKGNGD
                     GLHFAYSTDGLKWETVRNDSVFLKPAVGKDKLMRDPSIVRDNNGVFHMVWTSGWWDNG
                     IGYASSKDLIHWSEQKYIPVMEKFAVVKNTWAPELFYDRKDKLFYIFWASTIPGAFPE
                     IPTSESEKGLNHNLYYVTTKDFSTFSETKLYYNPEFSVIDGAMLAKDNKYYLFVKNEN
                     SNPPEKNIRVVSNSKPYNFPVKVSSPITGQYWAEGPAPLQVDEYVYVYFDKYRDKK"
BASE COUNT         1701 a         1174 c         1260 g         1382 t
ORIGIN      
        1 caatgaaaac ctgaaggcta atattgaaat atcgcatttt ggggaagagg tactgaaagg
       61 aaaacagacg gaatggaaag taaaaaatgc actgggaaaa acgatttcgc agggaatttt
      121 gtccgctaaa gatattcaga ttggcaactg ccaacagctt ggcgaaataa ccataccttt
      181 atcatttatc aaagaagccg aaaaaatgat cctggaagta aaactcggtg gaacagaagt
      241 ttccaacaac tgggatttct gggtctatcc ggaaatactg cctcaggtgg ataccacttc
      301 agtctatatc tgtgataaaa tggatgataa agccaaatcc gtgttgaata acggaggttc
      361 ggtattatta caactggctg gcaaggttac tcagggaaaa gatgtggtcc agcagttggt
      421 gccggcattc tggaatactt cctggttcaa aatgcgtccg ccacatacca ccggaattct
      481 gattaatgat tttcatcccg ttttcaggga tttccccacc gattattatt ccaacctgca
      541 gtggtgggaa ctggctaatc gggcacaggt gatggaactg accgatttcc cgaaatcttt
      601 tcaacccatt gttcaaacga ttgatacctg gtttatcaac cgccggttgg ccatgctttt
      661 cgaagccagg gtaggtaacg gaaagattat agtatgcagt gccgatattc aaaaagaccc
      721 ggacaaaaga attgttgccc gacagttgcg gtattctata ctgaaatata tgaattcgaa
      781 tttattttta ccggaaaatg agctaacttt ggaagtgatt gaaaacctga ccatcaaacg
      841 gggtgaatgg ttggatatgt ccatgacaaa ggatgcaccg gatgaattga agaagaatat
      901 ataatagttg aattcgattt tgagttatta agttattgag taattgagta attgaatttc
      961 tgaatcgccc cgccttttaa ggcggggata agagagaaaa atattatatt tctaaaaaat
     1021 aaatatacag caatgaaaaa taaatcctgc tgcttattaa tcatcgtgct ttttgcctca
     1081 atccatgcaa tggcatcgga cacaaactgg cctccggtaa ccaccgaaac aaaaccctgg
     1141 acacgctggt ggtggctggg aagcgctgta gacaaagaca atctgactta caatatctcc
     1201 gaactgggaa aagcaggcat gggtggcatg gaaattaccc ccatttacgg agtaaaaggg
     1261 aatgatgcca aaaacatcga ctatctctca ccacggtgga tgaatatgct cacacatacc
     1321 gaaaccgaag cgcgaaattc agggatgaaa gtggacatga acaccggtac aggctggcca
     1381 ttcggcggac ctgaagttac tttggaggat gctgcaacca aagctatttt tcaggaatat
     1441 aatgcgaatg gaggagagga tgtatcgctg aaaataacga tgaatgatcc gaaacaaaag
     1501 aaagtagcgt atctctcccg attgatggct tattccgata aaggcaaaaa actggatgtg
     1561 actaatttag tatcaaaaga gggcgacttc aagtggaatg ttccgaaagg tgactggaaa
     1621 ctcattgcat tgtttgttgg aaaaacgctg caggaagtga aacgctctgc tcccggcgga
     1681 caggggtatg taatggatca cttcagcgcc aaagcggtga agaattacct gactaaattt
     1741 gacaatgctt ttgcccgtga aggcacctca tacccaaact ctttcttcaa cgactcctac
     1801 gaagtgtata atgccgactg gacaccgact ttcctggaag agtttcagca gcgacgggga
     1861 tacaaactgg aagattattt cccggaattt ctggctaaag gaaaaactga catctcctgc
     1921 cgggtagttt ccgattatcg tcaaacactg agtgagatgc tgcttactga ttttacacaa
     1981 caatggactg cctgggcgca cagtcacgga gccattaccc gcaatcaggc acacggttct
     2041 cctgccaata tcatcgatct gtatgccgct gtggatattc ccgaatgtga gattttcggc
     2101 atatccgatt tcgatattcc gggactgaga aaagatgaaa tgaggaaaat aaatgacggt
     2161 gaccctgtta ctttgaaaat ggcaagctcc gcggcacaca ttaccggtaa aaagtttact
     2221 tcgtccgaaa ctttcacctg gcttaccgaa catttcagaa cttcgctttc gcagtgcaaa
     2281 ccggaaattg atcaggtctt tacatcgggt gtcaatcatg tgttttttca tggcactccc
     2341 tattcgcccc gcgaagctgc ctggcccgga tggctttttt atgcatcggt caatatgtcg
     2401 cccaccaatt ccatctggaa ggatgctcct gctttcttct cgtacataac ccgcgtacag
     2461 tcattcctgc agtacggcga accggataac gatttgctgg tgtatttccc ggtgtacgat
     2521 atctggtatg aacagcagag cgattattat tatgcattcg gcattcacgg attacgcgac
     2581 cggttaccaa agttttaccg ggcagttgaa aaaatcaggg aaagtggcag ggatgtggat
     2641 tatattaccg accgttacat tatgtcagcc accgtcgaaa acggtttgat aaaaaccatc
     2701 ggaggcacta cctacaaagc actggtacta ccggctgtac ggtttatgcc gctggaaact
     2761 gctcagaaat taacccaaat agccaacgat ggtgcaaaaa ttatttttgt tgatcattat
     2821 cccgaaggag ttcccggact gaaagatttc aataagcgtc aaagcaagtt gcataaactg
     2881 ataaacagat ttccgtcggg caatttcagt aaatcaaaag tcaaaaaaat tggaaaggga
     2941 cagattatca ccggtaagga ctatgacgaa ttactgacga tggcgaatat caaggatgaa
     3001 agtttctcga aagagttcaa aggacaatac atccgccgaa aaaacgaaac cggttaccat
     3061 tatttcttta cacagctcac caacaatcca gtcaacaatc ttgttgagtt gggggtagat
     3121 gcccgcagcg ctgtgatatt tgaccccctg accggcaaat cgggcaaagc gcaactgttg
     3181 agaagcagaa gaaacaggcc gctgctttgc ctgaatttaa aacccggaca atccatgata
     3241 gtgaaaacgt acaccaacga agatgtggat gtgccggact ggaactatta tcagattatt
     3301 ccatttaggt tattgaaacg aaatgaaggc tggaaactta attttatcca aagcgaacct
     3361 gccattaaag atacattcga tttgcagggc ttgggttcct ggacagaatt gagcaatccc
     3421 gatttgaaac gaaacatggg gaccggaaag tattctatca aatttaattt caaaaagacc
     3481 ggcaaagaat accggttgac gctgggcgat gtacgtgaaa gtgccagcgt gtatgtgaac
     3541 ggtcagtttg tggaaacttt attctccgta ccgttcgaaa caactatcgg aaaatacctg
     3601 aacgatggcg aaaacctgct cgaagtagag gtcaccaacc tccctgccaa tcgcatagcc
     3661 gattacgaca gacggggtgt ggattggcgc attttcaacg aaatcaactt tgtggatctc
     3721 aactaccgca aaaccctgta cgataaatgg gaaactgttc cctccggatt attgggaccg
     3781 gtggaaatat atgaaataac tccaaccaaa tcatttatgt tagattaatc aacttttaag
     3841 caaatcttat ttagtcgcag cgtatcgaaa atttcagaat taataaataa tcactaaacc
     3901 tcactctccc aagtcccctt caggggattt aggggcagtt cccttcagag ccggtcccga
     3961 aatggtttcg ggagggtcgg ggggccactc acattttatg aaaaaactca ttctatcact
     4021 cattatcctt gtacttctaa ccggtttcac ctcgtccgaa aaaaagataa agattttcat
     4081 agccggtgac tctactgcgc agacctacaa atcagacaaa gacggactga tcaggggctg
     4141 gggacaaatg ctcccgcttt tcctggacga gaaagtggaa gtggtcaatc acgccatggg
     4201 tggtcgcagc accaaaactt tcctcaacga aggccgctgg gacagactga tggcacaggt
     4261 gcgcaaaggc gattacgtgt ttattcagtt tggacacaac gatgcctcca cccgtcccga
     4321 acggtatgca tctcaagccg attattacga caatctggtg aaaatgatac aagatgtgag
     4381 agccaaaaag gcacatccgg tgctgttgac ttccgtggtt atacgcacct ttgtcaacgg
     4441 aaatctcacc gacgaccggc tgaaagccta tcccgtcatc atgcgtcaga tagccaaaga
     4501 atacaaggtt cagctcattg atgttaatca gaaaacccgc gatatggtta ctgtgctggg
     4561 tgataaaaag tcagttgctt attatcgtca caccgaaccc ggaatagacc ctgccaaacc
     4621 cgaaggtgta aaagatgata cacacatgat ggagaaaggg gcaactcagg tcgcttattt
     4681 tatagctgaa ggtatcatgg agcagaaaat gaaaggattg agcgaacatg tcaggctggg
     4741 tgctgatgat aaaagcatcc aatcctttat gaaaacaacc aaccagtacg tttatctgtt
     4801 ctcttatttc aagggaaacg gcgacggatt gcattttgct tatagtaccg atggactgaa
     4861 atgggagaca gtgcgtaacg attctgtgtt cctgaaaccc gcagtgggca aagataaact
     4921 catgcgcgac ccaagcattg tcagggacaa taacggtgta tttcatatgg tgtggacttc
     4981 gggatggtgg gataacggaa taggatatgc ttcatctaaa gacctgatcc actggtcaga
     5041 acaaaaatac attcccgtaa tggaaaaatt cgcagtagta aaaaatacct gggcaccgga
     5101 attgttttac gaccgcaaag ataaactgtt ctacattttt tgggcttcca ccatccccgg
     5161 tgcatttccc gaaatcccaa cttcggaaag tgagaaaggc ttaaatcaca atttgtatta
     5221 tgttaccacg aaggatttca gtactttcag cgaaacaaaa ctgtattaca atcccgaatt
     5281 tagtgtcatc gacggagcca tgctggctaa ggacaataaa tattatcttt ttgtgaaaaa
     5341 tgaaaattcc aatcctcccg aaaagaatat acgcgtagtg tcaaatagta agccgtacaa
     5401 tttccccgtg aaggtgtcct cgcccattac cgggcaatat tgggcggaag gtcctgcacc
     5461 gctgcaggtg gatgagtatg tatatgttta tttcgataag taccgcgaca aaaaata
//
LOCUS       DAOPYB010000202         5471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_18726_length_5471_cov_9.273080, whole genome shotgun sequence.
ACCESSION   DAOPYB010000202 DAOPYB010000000
VERSION     DAOPYB010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18726_length_5471_cov_9.273080"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /gene="bcp"
                     /locus_tag="PKH58_11690"
     CDS             complement(<1..455)
                     /gene="bcp"
                     /locus_tag="PKH58_11690"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNX89738.1"
                     /translation="MALQIGDKAPEILGQNQDGKEIKLSEFAGKKLVLYFYPKDNTPG
                     CTAQACSLRDEYSELRAKGYEVLGVSVDSTVSHQKFIKKYDLPFNLISDGDKTLSELF
                     GTWGEKKLYGKSYMGMLRTTFIISESGIIEKIILPKEVNTKEHGIQVMS"
     gene            complement(688..2025)
                     /locus_tag="PKH58_11695"
     CDS             complement(688..2025)
                     /locus_tag="PKH58_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNX89739.1"
                     /translation="MKAEQILNDLKRKFPNETEYHQAVQEVLESIEEVYNAHPEFDKQ
                     NLIERLCIPDRIFTFRVNWVDDKGNVQTNMGYRIQHNNAIGPYKGGMRFHASVSPSIL
                     KFLAFEQTFKNALTTLPMGGAKGGSDFSPKGKSNNEVMRFCQALMLELWRHIGPETDV
                     PAGDIGVGGREVAYMYGMYKKLARENTGTFTGKGLEFGGSLIRPEATGYGNIYFLMNM
                     LKTKGITDLKGQVVAISGSGNVATYTAEKVLELGGKVITLSDSDGYIYDPDGIDKEKL
                     AYVFELKNVYRGRIREYAEKYGCKYVAGARPWGEKCTIALPSATQNEINGDDAEILVK
                     NGCFAVSEGANMPSTPEAVDVFLKNKILYAPGKAANAGGVSVSGLEMTQNSMKLSWSK
                     EEVDQKLKTIMADIHSACVKYGTEPDGYVNYVKGANIAGFMKVARAMMAQGIL"
     gene            2279..2365
                     /locus_tag="PKH58_11700"
     tRNA            2279..2365
                     /locus_tag="PKH58_11700"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2313..2315,aa:Ser,seq:cga)
     gene            2425..2628
                     /locus_tag="PKH58_11705"
     CDS             2425..2628
                     /locus_tag="PKH58_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89740.1"
                     /translation="MSRNKLCPQCKIRRFQVKNAEGRSVVVTVNENYEIIPVHENESL
                     EGMDLSVLYCLGCSWKGSVKSLI"
     gene            2736..4973
                     /locus_tag="PKH58_11710"
     CDS             2736..4973
                     /locus_tag="PKH58_11710"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX89741.1"
                     /translation="MRNIGQCALSFILLFSGNLLLSQNLTGYVNPFIGTTNYGTTNPG
                     AVYPHGMVTVSPFNVTGYKENKYDKDAQWFSTPYEHTNSIFTGLSHISLSGVGCPELG
                     SLLLMPTTGKPDADIKNYGSNISRLKAEPGLFSCYLNRYNICSISSATQRTGITEFTF
                     PEGESNILLNLGEGLTNESGAFIRQVSDTEVEGMKLLGTFCYNPQAVFPVYFVMKVSK
                     TPKRMVYWKKQRPMTAEAAWDNTAGRFKFYEKYNREIAGDDIGIIYSYETQADEKITV
                     KIGISYVSIENARKNLVTEQPAFDIDKTIQSARSEWENILSRIKVEGGTSDQKTIFYT
                     ALYHLHIHPNILQDVNGEYPAMETGKTLRTAGNRYTVFSLWDTYRNVHPLLSLLYPDI
                     QLDMVRTMLDMYRESGWLPKWELYGRETLTMEGDPSLVVLNDTWQRGIKDFDTTLAYE
                     AMTKSANMEGSRNLMRPDNDDYLHLGYVPLREKFDNSVSHALEYYLADWNLAQFAKSQ
                     GKMEDYMSFLKRSLNYRNYFDSETNILRPKLPDGKFLVPFNPSQGANFEDVPGFHEGS
                     AWNYAFYIPHDITGLSKLMGGKKKFVDQLQQVFDKGYYDMANEPDIAYPYLFNYFKSE
                     EWRTQKEVTRLIDKHFTNAPNGLPGNDDCGTMSAWLVYSMMGFYPVCPGDMNYALTTP
                     VFDKITIRLDPKYYSSTEIVIEKEKNKSATTPLRFITADGRKLNRFFINHHQLTSLKR
                     LVYHF"
     gene            5056..5131
                     /locus_tag="PKH58_11715"
     tRNA            5056..5131
                     /locus_tag="PKH58_11715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5092..5094,aa:Pro,seq:ggg)
     gene            complement(5209..>5471)
                     /locus_tag="PKH58_11720"
     CDS             complement(5209..>5471)
                     /locus_tag="PKH58_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNX89742.1"
                     /translation="FTIPENNLILAKDSLQGIFGTGHNSVVQVPGKDEWYIVYHRFSY
                     PDGIKMGDAAGFNREVCIDKLEFNTDGSIKPVKPTHKGVKLE"
BASE COUNT         1596 a         1138 c         1096 g         1641 t
ORIGIN      
        1 aaactcatca cctgtatccc atgttctttg gtattcactt ctttcggtaa aatgattttt
       61 tcgatgatgc cgctctcact gatgataaag gttgtacgaa gcatgcccat gtaagattta
      121 ccgtaaagtt tcttttcgcc ccacgtcccg aacagttcag aaagtgtctt gtcgccatcg
      181 ctgatcagat tgaaaggtaa gtcatatttt ttgataaatt tctgatgaga aactgtactg
      241 tcaacgctta caccgagcac ttcataaccc tttgctcgca attcagaata ctcgtcgcgc
      301 agactgcatg cctgtgcggt acatccggga gtattatctt tcggataaaa gtaaagaacc
      361 agttttttgc ccgcaaactc ggaaagtttg atttctttgc catcctggtt ttgaccaagg
      421 atttcggggg ctttgtcccc aatttgtaat gccataataa tgtagttttt tatcagatca
      481 atagattgta attaatttcg gtaaacagat atgttgaata tttttaatac cgcctgaaat
      541 aacgaattca atacaggaaa tgttttgttt gctgctttat tttttttctt tatgcagctg
      601 tttgcaaata aaaaaatgct caaccaaagc agagcatttt tttatatagt attaactgta
      661 tattactgat ttaatcacta atcactatta aagtattccc tgagccatca tggcacgtgc
      721 aactttcata aatccggcga tatttgcgcc ttttacatag ttgacataac catcgggttc
      781 agtaccgtat ttcacgcagg cagagtggat gtcggccatg atagttttca gcttttgatc
      841 cacttcttct ttcgaccagc tgagtttcat tgagttctgt gtcatttcca gtccggaaac
      901 agatacacct cccgcattgg cagctttacc cggcgcatac agtattttgt ttttcagaaa
      961 tacatcaaca gcttcaggag ttgaaggcat gttagctcct tcggatacgg caaaacaacc
     1021 attttttacc agtatttcgg catcgtcacc gtttatttca ttctgtgtgg ccgatggaag
     1081 tgcaatggta catttttcac cccatggacg tgcgcctgca acatatttac aaccatattt
     1141 ctccgcatat tcacggatgc ggccacggta aacatttttc agttcgaata cgtaagccag
     1201 tttttcttta tcaataccat caggatcgta aatatatccg tcgctatctg atagtgtaat
     1261 aacttttcca cccagttcca gtactttttc tgccgtgtaa gtggcaacgt ttcctgaacc
     1321 tgaaatagca acaacctgac ccttcaggtc tgtaatgcct ttagttttca gcatattcat
     1381 caggaagtaa atgttaccat aaccagtagc ttcggggcgt ataagcgaac caccgaattc
     1441 cagacctttt ccggtgaatg ttccggtgtt ttcgcgggct aattttttgt acatgccata
     1501 catataagcc acttcgcgac ctccaacacc tatatcaccg gcaggaacgt cagtttcggg
     1561 gccaatgtga cgccacaatt cgagcatcaa tgcctgacag aaacgcatta cttcattatt
     1621 cgatttccct ttagggctga agtctgaacc gccttttgca ccacccatag gaagtgtggt
     1681 cagcgcattt ttaaatgttt gctcaaaagc aaggaatttc aaaatggaag gacttacaga
     1741 agcatggaaa cgcataccgc ccttgtaagg gccaatggca ttattatgct ggattctgta
     1801 acccatgttt gtctgaacat tacctttatc gtccacccag tttacacgaa aagtaaaaat
     1861 acgatccgga atacaaagac gttcaatcag attttgttta tcaaactcgg gatgagcgtt
     1921 ataaacctct tcgatagatt ccaatacttc ctgtactgcc tgatgatatt ccgtttcatt
     1981 cggaaatttt ctctttaaat catttaaaat ttgttctgct ttcatttttg taaaatagtt
     2041 ttgagttttt attgtttcat tgcaaatata caataattaa ccgaaataat gaatataaat
     2101 ttgaaaataa tggagtaata atgataaaat gataatttgt gtgtataaaa ataccaaaat
     2161 taattggttt tattttgaaa aaaaaaggtt taacgatatg atgtaaaaaa atctttcgca
     2221 acaaaaatca aaatattgcc aatatctgaa gaaaaaaaat tatttttgta ctccctaagg
     2281 aaagctgccg gagtggtcga tcgggcttga ctcgaaatca agtgtacggg taactgtacc
     2341 gggggttcga atccctcgct ttccgcaata atataacaat cccgaagcgc ataccgttta
     2401 cgggatttta ttttaaaaaa caatatgagc agaaataaac tttgtccgca gtgcaaaatt
     2461 cgtcgctttc aggttaaaaa cgctgaagga aggagtgttg tcgtaactgt gaacgaaaat
     2521 tacgaaatta tccctgttca tgagaacgaa tcactcgaag gcatggatct gagtgtgctt
     2581 tattgcctgg gctgttcgtg gaaaggctct gtgaaatcgc tgatataata gaacggtttc
     2641 atcatatctt aaaaacattt ctttaacatg atttctctga ataatttaat tattcaaact
     2701 cttcaaaaat cataaacctt taaagtattc ctgctatgag aaacattggt caatgtgctt
     2761 tgagttttat cctgcttttt tccggtaatc tcctgctttc tcaaaatctc accggttacg
     2821 ttaatccgtt tattggtaca actaattacg gtaccaccaa tcccggagct gtatatccgc
     2881 atggcatggt cactgtcagc ccctttaatg taacgggcta caaggaaaac aagtacgata
     2941 aggacgcgca atggttttca actccatatg aacataccaa cagcattttc accggtttat
     3001 cgcacatcag tttgagcggg gtgggttgcc cggaactggg tagtttgctt ctgatgccta
     3061 ctaccggtaa gccggatgcc gacataaaaa actacggcag taatatcagc cggttgaaag
     3121 ctgaacccgg attgttttca tgttatctga accgctataa tatttgtagt atcagttcgg
     3181 caactcagcg aacgggcatc acagaattca ctttcccgga aggtgagagt aatattttgc
     3241 taaatctggg tgaggggctg acaaatgaaa gcggagcttt tatccgtcag gtgagtgaca
     3301 ccgaagtgga agggatgaaa ctgctgggta cgttctgcta caatccgcag gcagtgtttc
     3361 cggtgtattt tgtaatgaag gtgagtaaaa cgccaaaacg gatggtttac tggaaaaaac
     3421 aacgaccgat gacagccgag gctgcctggg ataacactgc cggaaggttc aaattttatg
     3481 agaaatataa ccgggaaata gccggagatg atattggcat tatttacagc tatgaaacac
     3541 aggctgacga gaagattact gtaaaaattg gaatttcgta tgtaagtatc gaaaatgccc
     3601 gaaaaaacct ggtaactgaa caacctgctt ttgacattga taaaacgata caatcagccc
     3661 gctcggagtg ggagaatata ctttcgagaa taaaagtgga aggcggcacc agcgatcaga
     3721 aaactatttt ctacacagcc ctttatcatc ttcacattca tccaaatata cttcaggatg
     3781 taaatggtga atatccggct atggaaaccg ggaaaacgct ccgtactgcc ggaaatcgtt
     3841 atacagtatt ttcgctctgg gatacctacc gtaatgtgca tccgctcctt tcgttgctat
     3901 accccgatat acagctggat atggtacgta caatgctaga tatgtaccgc gaaagcggat
     3961 ggctgcccaa gtgggaactt tatggtcgcg aaacgcttac catggaaggc gatccgtcac
     4021 tggttgtact caacgatacc tggcagcgcg gaatcaagga tttcgatacc actcttgctt
     4081 acgaagccat gacaaaatca gcaaacatgg aaggctcacg caaccttatg cgacccgaca
     4141 acgatgatta tctgcatctg ggttatgttc ccctgcgtga aaaattcgac aattcagttt
     4201 cacatgcgct tgagtactat cttgccgact ggaacctggc tcagtttgcc aagtcacagg
     4261 ggaaaatgga ggattatatg tcttttttaa aacgctcgct gaattacagg aattatttcg
     4321 attccgaaac caacatctta cgtcccaaac tgcccgatgg taaatttctg gtgcctttta
     4381 acccttccca gggagctaat tttgaagatg ttcccggatt tcacgaaggc agcgcctgga
     4441 actatgcttt ttatattccg catgatatta ccggtctgtc aaaactgatg ggtggaaaaa
     4501 agaaatttgt ggatcagctt cagcaggtct tcgataaagg gtattatgat atggccaatg
     4561 aaccggatat cgcttatccg tatctgttca actatttcaa aagtgaagaa tggcgaactc
     4621 agaaggaagt tacccgcctg atcgacaagc attttaccaa tgcacccaat ggccttccgg
     4681 gaaatgatga ctgcggaacc atgtcagcat ggcttgttta tagcatgatg ggattttatc
     4741 ccgtttgtcc gggtgatatg aattatgcgc tcacaactcc tgtgtttgat aaaatcacta
     4801 ttcggcttga tccaaagtat tattcatcaa cggaaatagt catagagaaa gaaaaaaaca
     4861 aatcagcaac aacaccgtta aggtttatta ctgccgatgg ccgaaaactg aaccgttttt
     4921 tcattaatca tcatcaatta acaagcttga aaagactggt ttatcatttt tgagttataa
     4981 tttttaaaca ggaattacag atttcttttt taaataagaa aaaaatatta tctttgcatc
     5041 acaaatcaaa gtcgacgggg tgtagctcag cccggtttag agtactcgtc tggggggcga
     5101 gtggtcgcaa gttcgaatct tgtcaccccg actaatgata taaaaaaaga ctttcgcgat
     5161 tgcgaaagtc ttttttatgt ttacaggtct tcacttcgac gtgattgatt attctaattt
     5221 cacccccttg tgggtcggtt ttacaggttt gatggaacca tctgtattaa attcaagttt
     5281 atcgatgcaa acttcccgat tgaaaccggc agcatctccc atctttatgc catccggata
     5341 gctgaaccgg tgataaacaa tataccactc atctttgccg ggtacctgga ccacagaatt
     5401 atgtccggtt ccaaaaatac cctgcaagct gtctttcgcc aaaatcaggt tgttttccgg
     5461 tattgtaaat t
//
LOCUS       DAOPYB010000203         5432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_18925_length_5432_cov_9.565557, whole genome shotgun sequence.
ACCESSION   DAOPYB010000203 DAOPYB010000000
VERSION     DAOPYB010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18925_length_5432_cov_9.565557"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..805)
                     /locus_tag="PKH58_11725"
     CDS             complement(149..805)
                     /locus_tag="PKH58_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX89743.1"
                     /translation="MKTKLISITFTLLLFATIASAQISVGVLGGVNFQNLNGKNSSGD
                     ALENTMIPGFHAGVNVQIPIAPEFYFQPGLMFSTKGAKNVGSILTTNTNLSYVELPLN
                     FLYKGQLGSGFVFIGVGPYVAYAVKGKVKTEGGSVSLDSPVKFQNVVEITDTQYPYYK
                     ALDAGGNILFGYELSGGIFAQVNAQLGMLNINPEYKILTNDKSVVKNTGFGVSLGYRF
                     "
     gene            complement(896..1561)
                     /locus_tag="PKH58_11730"
     CDS             complement(896..1561)
                     /locus_tag="PKH58_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX89744.1"
                     /translation="MKKLSFIMILSATLFMIGCSSLNKTQKGAIIGTAGGGAAGAVIG
                     KVAGNTALGAIIGAAVGGASGAIIGHQMDKQAAEIAKTVPDANVVRVGEGIVVEFSSN
                     VLFGFDQTNITSDARSNLDKLVKVLNTYPETNIEIQGHTDSKGSLAYNKTLSQQRSAA
                     VSNYLISQNISANRLSNVGYGETLPKYQNDTESDRALNRRVEFLITANEKMKAEAQKE
                     AAK"
     gene            complement(1814..2167)
                     /locus_tag="PKH58_11735"
     CDS             complement(1814..2167)
                     /locus_tag="PKH58_11735"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX89745.1"
                     /translation="MQNKVDNIELEKGKSHIVVEIIEYMPNAVVSKTIIKKLSGNVTV
                     SSLDAGEELAEKISPFDTYIQIIDGAAELTIDHKKMKLKLGDGIIIPAHSRHRFNANE
                     QFKMIATVIKSGYED"
     gene            complement(2279..2707)
                     /locus_tag="PKH58_11740"
     CDS             complement(2279..2707)
                     /locus_tag="PKH58_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX89746.1"
                     /translation="MKKKIKIFLVDDDVVFLRLLEIQFNESGDFEIETYSTGELCVEN
                     LSHKPDVIILDYLLDGVVRDVMNGIETLDKIKAYDTNIPVIILSAQDKIDVAINCMHH
                     AAFDYVVKSETSFLRLQKIITTIFETSKLKKQLNWYMERM"
     gene            complement(2704..4698)
                     /locus_tag="PKH58_11745"
     CDS             complement(2704..4698)
                     /locus_tag="PKH58_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX89747.1"
                     /translation="MEEQASQYARSLIEASLDPLVTISAEGKIMDMNRATANIIGLTH
                     DEITDSDFFDYFTEPHKAREVYQEVFEKGSVADYPLTLRHVTGKLTDVLFNGSVFRDE
                     KGNVLGVVIVARDVTDQKRIACELTEAKEFAEKANEQAQIAKETAQLATHKAEDAVKA
                     KQQFLSNMSHEIRTPMNAIIGFTKVLLKTELNAKQKEYLTAIKMSGDALIVLINDILD
                     LAKVDAGKMTFEQIPFRPKLSISAMLQLFKQKILEKNLKLEVEYDNNIPDVLLGDPVR
                     LHQIILNLVSNAIKFTNAGSIKVSVKLVSENDEQAKLQFSVADTGIGIEKAKLGKIFE
                     NFQQGSSDSSRLYGGTGLGLAIVKQLVEPQGGIIHVESELNKGSVFSFTLDFPKTDQT
                     PDSELEIMELNGDARNINVLVAEDVPLNQLLMKTILDEFGFKCDIAENGKIAVEKLKV
                     NDYDVVLMDLQMPEMNGFEATGYIRNRLKMQIPVIALTADVTTVDLAKCKAVGMNDYI
                     AKPVDERLLYSKIVSLVKQSQTSGEQNENDDYRKIEGKCTDLVYLMQRTKSNPTLMME
                     MISLYLEQTPPLVDAMKLSMKNKDWNSLYSAVHKMIPSFSIMGINHDFENMAKKVQEF
                     ASNQQQTDGITELVLKLENVCTQACKELETEYNVLKNMNA"
     gene            complement(4722..5282)
                     /locus_tag="PKH58_11750"
     CDS             complement(4722..5282)
                     /locus_tag="PKH58_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX89748.1"
                     /translation="MKIYIKYMVSLRCKMVVKEELKKLGLHFIVVDLGEIEIMEDLTE
                     SQHDALKEALLNSGLELMDDKRAVLIEKIKNLIIEMIHYADDLPKINYSDYISEKLHY
                     DYTYLSNIFSEVKGITIQQFIILHKIERVKELMLYEELNLTEISYRLQYSSVAHLSNQ
                     FKKITGLTPSQFKLLKNRHRDPIENI"
BASE COUNT         1394 a         1111 c         1037 g         1890 t
ORIGIN      
        1 taaataataa ataagaaaga tgaaccgaaa acccgtaaag tttaattgta aaaatgaaga
       61 aacgcaaagc gcaaaaatca atttatcacc ctgcgtttct gtaagtttac gtttttactg
      121 taaacttatg agctattaat cctgatgctt aaaatcgata tcccagcgaa acgccgaaac
      181 cggtgttttt caccacggat ttatcattcg tcagtatttt gtattcagga ttgatattga
      241 gcataccaag ctgggcattt acctgagcaa atattccacc tgacaattcg tatccgaaaa
      301 gtatgttacc tccggcatcc agtgccttgt aataaggata ttgagtatca gtgatttcca
      361 ccacattttg aaatttaacc ggactgtcga gtgatactga gccaccttcg gtttttactt
      421 tacccttaac agcataagcc acataaggac ctacaccgat aaatacaaag ccgcttccga
      481 gctgaccttt gtacaggaag ttcaatggaa gttcaacata cgatagattg gtgttggtgg
      541 ttaagatact acccacgttt ttagctcctt tggtactgaa catcagtccg ggttggaaat
      601 aaaattcggg tgcaatcggg atttgaacgt tcactccggc atgaaatccc ggtatcattg
      661 tattttcaag ggcgtcgccg cttgagtttt tcccgttcag gttctgaaaa ttcactccac
      721 cgagcacccc caccgaaatc tgagcgctgg caatagttgc aaaaagtaaa agtgtgaatg
      781 taatcgaaat tagttttgtt ttcattttta tttttatttg tattaatgaa tttgttagtc
      841 tttattcttt attctttgtt ctttgttctt tgctctttgc tctttgctct tttatctatt
      901 tagctgcctc cttttgagct tcggctttca ttttttcgtt ggctgtaatc agaaattcca
      961 ccctgcggtt tagagcgcga tcgctttcgg tatcattctg atattttggt aacgtttcac
     1021 catatcctac gtttgacaac cggttggctg atatgttttg tgaaatcagg tagtttgaaa
     1081 ctgccgcaga acgttgttgt gacaatgttt tgttgtatgc cagacttcct ttgctatcgg
     1141 tatgtccctg tatttctata tttgtttcgg gataggtatt cagtactttc accagtttgt
     1201 ccagatttga gcgggcatcg gatgtaatgt ttgtttgatc aaaaccaaaa agtacgttac
     1261 tgctgaattc cactacaatt ccttcgccaa ctcttactac attagcatcc ggaacggttt
     1321 tggctatttc ggcagcctgt ttatccattt gatgtccaat gatggcacct gaggcacctc
     1381 ccacggcagc tccgataatt gcacccaggg ctgtgttacc ggctactttg cctattactg
     1441 caccggcagc tccaccgcct gcagtgccta taattgcacc tttttgtgtc ttgtttaatg
     1501 acgagcaacc tatcataaac agagttgcac ttaaaatcat gataaaactt agttttttca
     1561 ttgttatatt ttttagttgt tagttttgat tttcggtatt gtttattgtt actgattaac
     1621 cgaatttatt cttagcaaag gtggggtaaa ttgctccgaa agtcgttaca gatttctgag
     1681 aataagttac aacattcacg cttttacaca gcaaaagagc caatcacgtg tgatcagctc
     1741 tttagtaata gattgtaaat gagataaata tgggttatga cttataattt ataacttata
     1801 atttataact gacttagtct tcatatccgc tttttattac agtcgctatc attttgaatt
     1861 gttcgttggc attgaaacga tgcctggaat gtgcaggaat aatgatgcca tcacctaatt
     1921 tgagtttcat ttttttatga tcaatagtca gttcagctgc accatcaatg atttgaatat
     1981 aggtatcgaa aggcgaaatt ttttctgcta attcttcacc ggcatcaagg gatgaaacag
     2041 taacattacc cgacaatttt ttaattatcg ttttactgac cacggcattg ggcatatatt
     2101 ctataatttc gacaacaata tgcgatttcc ctttttccaa ctctatattg tccaccttgt
     2161 tttgcatgat attgtttctt ttgaaaattt acaaatattt gtattacaaa gataaaatca
     2221 cgccgatgaa aataaattac atatctgcct gaatatgtta catagttcac tgattacgtt
     2281 acatccgttc catgtaccag ttcagttgtt tttttagttt cgaagtttca aaaatggtgg
     2341 taattatttt ttgcaggcgc aaaaatgaag tttcactttt taccacataa tcgaacgcag
     2401 catggtgcat acaattaatc gccacatcaa ttttatcctg agccgaaagt atgattacag
     2461 gtatattggt atcataagct ttaattttat ccagggtttc tataccattc ataacgtctc
     2521 ttaccactcc gtcgagcaga taatccagga taataacatc gggtttatgc gataaatttt
     2581 ccacacaaag ttcaccggtg gagtatgtct cgatctcgaa atcgccactt tcattaaact
     2641 gtatttcgag taaccggaga aaaaccacat catcatctac caaaaaaatc tttattttct
     2701 ttttcatgcg ttcatgtttt ttaaaacgtt atattccgtt tccagttctt tacatgcctg
     2761 cgtgcaaaca ttttcaagtt taagaaccag ttcggttatt ccatcggttt gttgttggtt
     2821 actggcaaat tcctgcactt ttttggccat gttttcgaaa tcgtgattaa tgcccatgat
     2881 tgaaaatgaa ggtatcatct tatgcacagc cgaatacagc gagttccagt ctttgttttt
     2941 catgctaagt ttcatggcat ccaccagggg cggcgtttgt tccaaatata gcgagatcat
     3001 ctccatcatc agggtggggt tcgattttgt gcgttgcatc agatacacca gatcggtaca
     3061 tttaccttct atttttcggt aatcatcatt ttcattctgt tcgccggagg tctgggattg
     3121 ttttaccagg cttactattt tgctgtacaa caggcgttcg tccaccggct tggcaatata
     3181 atcgttcatt cccactgctt tgcatttggc cagatccacg gtggttacat cggcagtgag
     3241 cgctatcacc ggaatctgca tttttaagcg gttgcggata tatcccgtag cttcaaatcc
     3301 gttcatctcg ggcatttgga ggtccatcag cacaacatcg tagtcattca ctttcagttt
     3361 ttcaacagct atctttccat tttcggcaat atcacattta aagccaaatt catcgagtat
     3421 ggttttcatc agcagctgat tgagcggtac atcttcggcc accagtacgt tgatattcct
     3481 ggcatctccg tttagttcca ttatttccag ctcgctgtcg ggagtttggt ctgttttagg
     3541 aaaatcaagc gtaaaactga aaacagaacc tttatttaat tcgctttcca cgtggattat
     3601 accgccttgc ggttccacca gttgctttac aatagccaat cccagtcctg taccaccgta
     3661 aagtcgcgaa ctgtcacttg atccctgctg aaagttttca aatatctttc cgagtttggc
     3721 tttttcaatg ccaattccgg tatcggccac cgaaaattga agttttgcct gttcgtcgtt
     3781 ctcactcact aattttacgc ttactttaat gcttccggcg ttggtaaatt tgatggcatt
     3841 actgaccaga ttgagaataa tctgatgcaa tcgcaccgga tcaccaagca gaacatcggg
     3901 aatattgttg tcgtattcca cttccagttt caggtttttt tccagaattt tctgtttaaa
     3961 caattgcaac atggccgata tcgataactt cggcctgaaa ggaatttgct caaaagtcat
     4021 ttttccggca tccacttttg caaggtcaag aatatcgtta ataagcacta tcagtgcatc
     4081 gccgctcatt tttatggcag tgagatattc cttttgttta gcattcagtt cggttttcag
     4141 cagcactttt gtaaacccga taatagcatt catcggggtt cggatttcgt ggctcatatt
     4201 cgagagaaac tgttgtttgg ctttcaccgc gtcttcggct ttgtgagtag ccagttgggc
     4261 tgtttctttg gcaatttgtg cctgctcgtt tgctttttcg gcaaattcct tggcttctgt
     4321 cagctcgcag gcaatgcgtt tctgatccgt tacatcgcgt gccacaatta ccactcccaa
     4381 tacatttccc ttttcatccc tgaaaacaga accattgaaa aggacatcgg tgagtttgcc
     4441 cgtaacatgc cgcaaggtga gtggataatc agccaccgaa cctttctcaa aaacttcctg
     4501 gtacacttct cgtgctttgt gcggttcggt aaaataatca aaaaaatcgc tgtcggttat
     4561 ctcatcgtgg gtcagaccaa taatattggc cgttgcccgg ttcatatcca taattttacc
     4621 ttcggcactt atggttacca aagggtccag actggcttct atcagactgc gtgcatattg
     4681 tgatgcttgt tcttccattt tttgtgttag atatttcttt cttagatatt ttcaatcggg
     4741 tctctatgtc tgtttttcag cagtttgaat tgtgagggtg taagtccggt tattttttta
     4801 aactggttgg ataaatgtgc tacgctgctg tattgaagcc ggtacgaaat ttcggtgagg
     4861 ttcagctctt cgtacaacat cagttccttg acccgttcta ttttatgcag aatgataaat
     4921 tgctgaatgg ttatgccttt cacttccgaa aatatattgg agagataggt ataatcgtaa
     4981 tgcaattttt cgcttatata atcggaataa ttgattttgg gcaaatcatc ggcataatgt
     5041 atcatttcaa tgatcagatt tttaattttt tcaatcagta cagcccgttt gtcgtccatc
     5101 agttcgagcc ccgagttaag caaggcttct ttcagggcat cgtgctggct ttcggttagg
     5161 tcttccatga tttctatttc gcccagatcc accactatga aatgaagtcc gagttttttc
     5221 agctcttctt tcaccaccat tttgcagcga aggctcacca tgtatttgat ataaattttc
     5281 attcttcttt tttaagtgaa tactggaaac gaaacgggaa atgtgtaatg tttaatgtgt
     5341 agtgtgtaat gtgtaatgtg taatgtgtaa tgtgtaatgt gtaatgtgta atgtgtaatg
     5401 tgtaatgtgt aatgtgtaat gtgtaatgtg ta
//
LOCUS       DAOPYB010000204         5285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_19692_length_5285_cov_10.032122, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000204 DAOPYB010000000
VERSION     DAOPYB010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19692_length_5285_cov_10.032122"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKH58_11755"
     CDS             <1..250
                     /locus_tag="PKH58_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89749.1"
                     /translation="VDLNSDKFKYLDGYVLNNNRLIVSNLTPSDGVMYLYDISGKALM
                     NKKIPTSVSEIPVSLETGVYILNLQADGKYETVKLIIK"
     gene            259..918
                     /locus_tag="PKH58_11760"
     CDS             259..918
                     /locus_tag="PKH58_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89750.1"
                     /translation="MNTRKRLSEIILLTLAVVVGGGFLYFSKLSEIRKDPGTISRIYL
                     QKAESPTVQLNNVNSKKRFTTNRSNQYSIRKPCVVETPLYNEVIEAGVSAAGSIQSGN
                     TNYTYIRKNRDARSNYQGGSFGSAEILAYGRRGSQTDGEVSTQGTGGITLTETGATSP
                     FAVPKTATENPPSSAGGNILVDPMTDPIVTNQIPVNDGVGLLIMLISAYAGWISFRKR
                     K"
     assembly_gap    1094..1193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2118..2909
                     /locus_tag="PKH58_11765"
     CDS             2118..2909
                     /locus_tag="PKH58_11765"
                     /inference="COORDINATES: protein motif:HMM:NF040365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6261 family protein"
                     /protein_id="HNX89751.1"
                     /translation="MLQILIFAFTKLHKLEFAYFLKGVIRIVEKHNPSLLKILGFYNR
                     LLELEPQLDGLTVKYGKHAISKELSDLRVERHGLLLAITGQIHSVENANLKAHENELR
                     YALPVLKRFFTDILRKNEKDKSERIEQFIRFAKTDTVLNAALKTLGFELYVAELAEIT
                     DKMRQKHITRTEEISARPKMNTVERVAMLNESVIRLFQAIELGAIENPDLDFQPLISE
                     LNVWISYYHTLVKSRQTRSANKTEMDELEIKKAAAMSDKTSATAL"
     gene            complement(3380..3452)
                     /locus_tag="PKH58_11770"
     tRNA            complement(3380..3452)
                     /locus_tag="PKH58_11770"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3417..3419),aa:Phe,seq:gaa)
     gene            3727..5235
                     /locus_tag="PKH58_11775"
     CDS             3727..5235
                     /locus_tag="PKH58_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNX89752.1"
                     /translation="MTISTELFLLVASVLLFFSLLIGKTGHKYGVPTLLLFLMIGIMA
                     GTDGLGLEFNSPGITQFIGVIALNIILFSGGMDTNLYEIKPIVTQGLVLATVGVLITA
                     LLTGLFIFWITNSIFTSVSFTLLESLLLGSIMSSTDSASVFSILRSKGLSLKENIRPL
                     LEFESGSNDPMAFMLTIVLIQLILTPDFSTGEAVFMFFRQLILGAIGGLFLGKLSVKI
                     INRINLDNDALYSVLLLTIMFFLFGFTSFVGGNGYLAVYAGGLLIGNHKFVHKRSSLK
                     FFDGLTWLFQIIMFLTLGLLVNPGELLPIAGVALLIGVFMLLVSRPAAVFISLTPFRK
                     LTFKARTYVSWVGLRGAVPIIFATYPWIEGVPKAKMLFNIVFFITILSLVIQGTTVGT
                     MAHWMKLSLPVPKKRKLREFDVEFSDEIKSAMSEISIKEEFLANGSRLMDINVPDKTL
                     VAMVKRKGKYFIPRGNTELLVGDRILVITDDEKALEETYQSMKGKLSETSPL"
BASE COUNT         1551 a          968 c         1106 g         1560 t
ORIGIN      
        1 cgttgacctc aacagcgata agttcaaata tcttgacggt tatgtactga acaacaaccg
       61 gctcatcgtc agcaacctga caccgtccga tggggttatg tatttgtacg acatatcagg
      121 gaaagcactg atgaacaaaa aaattccgac ctcagtaagt gaaattccgg tttctcttga
      181 aacgggcgta tacattctga atttacaggc tgatgggaaa tacgaaacag ttaaactgat
      241 tatcaaataa acggagatat gaatacgaga aagagattat cagaaattat attgctcacg
      301 ctggctgttg tagtgggagg tggcttcttg tatttttcaa agctttcgga gatccggaag
      361 gatccgggaa cgatttcacg tatttacctt cagaaggctg aaagcccgac tgtacaactg
      421 aataatgtaa attcgaagaa aaggtttacc actaaccgaa gtaatcagta cagcataaga
      481 aagccctgtg tagttgaaac cccgctctat aacgaagtaa ttgaagctgg tgtttctgct
      541 gcaggaagca ttcagtcggg taacaccaat tatacttaca tccgaaaaaa cagggatgca
      601 agaagcaact atcagggcgg aagctttggg tcagcagaga tcctggctta cggcaggcgt
      661 ggtagtcaaa cggatggtga agtgtcaacg caaggtaccg gagggataac cctgacagaa
      721 accggagcca catcgccctt tgctgtaccg aaaacagcaa ctgaaaatcc accttccagc
      781 gctggcggaa acatcctggt ggatccgatg accgatccga ttgtaactaa tcaaattccg
      841 gtgaatgatg gagttgggtt actgattatg ttgatctcag cttatgccgg atggattagc
      901 ttcagaaaac gaaaataaca gcggaatagc ttaccaaaat taattcatca ataagacctg
      961 acagggtttc aatacctgtc aggtcttgtt atttaagaac tcagaccctg aaagtaccgg
     1021 gtattttcag gtttttctat atgactgccg aaaaattatt aagttttttc cccggaacat
     1081 aaggtcactc tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcaggta
     1201 gaagcaggaa ttcccctgag cgaaggtcac tctgttgaag atgaatcagt cttaactcct
     1261 gagcaaaggt cactctgctc aggtagaagc aggaattccc ctgagcgaag gtcactctgt
     1321 tgaagatgaa tcaatcttaa ctcctgagca aaggtcactc tgctcaggaa gaagcaggca
     1381 cttctccgga gctaaggtta aactgctgaa gatgaagcga acttttcacc gaagttaagg
     1441 ttaaactgcc gaagatgaaa tgagactttc accggatcaa aggtcactct gcacaggatg
     1501 aagtgagttt ttccccggag taaaaatcaa tctgccggaa agtaagcttt gattttatca
     1561 taaccaaaca ggattagtat cgttaaatgt gaacgtatat ccggttttat agcatatttt
     1621 ttgaaacttc tgtacattag ttacatattt tccccgtact gaaacggaag ttaactgagg
     1681 taaatatctg atgaattcct gctaaactca ggtaaatgag tgttaaacag gtaaacgata
     1741 tctcatcatt ttgttgaaaa aaaacacttt taggacacat tatttcaatt tgcacataaa
     1801 aacaaatatt tattttgaaa atattttgtt gtaatttgga atttttaatg ctatgatata
     1861 caggattata gcgcattata ttgtgatttt tacataagtt tctcgtaact ttttaaaaaa
     1921 tgtttgtgaa ttaagaaaat aatccttaaa attgtgagcc gacttagtga cactacggtg
     1981 aaagaagttc gaccaattgc gaagcgtcgt gattgcattt tctttagttg gacaaacagg
     2041 cagttcctgc ttttggtgtg aatttcgatt aatcgcgatt gtatttgttt tttttgttaa
     2101 tttaatacaa aatcagtatg ttacaaatac ttatattcgc ttttactaaa cttcataaac
     2161 tggagttcgc ttattttttg aagggtgtca tcaggattgt tgagaaacac aacccttctt
     2221 tgcttaaaat tcttggattt tacaaccgat tgttggaatt ggagcctcag cttgatggat
     2281 tgaccgtaaa atacggtaaa catgcgattt cgaaggaatt atctgacttg agggtggagc
     2341 ggcatggttt gctgctagcc attaccggtc agattcattc tgtagaaaac gcgaatctga
     2401 aagctcatga aaatgaattg cgctatgcgc tgcccgtgtt gaaacgtttt tttaccgata
     2461 ttttgagaaa aaacgagaaa gacaaaagcg agcgtattga acagtttatt cgatttgcaa
     2521 aaacagatac cgtgttgaat gccgcgctga aaacgttggg ttttgaactc tacgtagccg
     2581 aattggcgga aatcacagac aagatgaggc agaagcacat cacacgtacg gaggaaattt
     2641 cggctcgccc gaaaatgaac accgttgaac gtgtggcgat gctgaacgaa tccgtgatca
     2701 gattgtttca ggccattgag cttggagcca ttgaaaatcc ggatttggac ttccaaccac
     2761 ttatttcgga gctgaatgtg tggatatctt actaccatac gctggttaag agccgtcaaa
     2821 cacgaagcgc caacaaaact gaaatggatg aattagagat aaaaaaagca gccgcgatgt
     2881 ctgacaaaac ttccgcgacc gccttataag ccgaaacctc attttagtta tgaaagtccc
     2941 gtatgcctgt tatcggggct tttttttgcc cctgtgttat gcaaagtaat taaaaattcg
     3001 aattggtgta ttaaaagggt ataaactgca atctaatgcc ctgtaatgac gtatcgtatg
     3061 tgatattgtg ttaatttagc actaaaatga gtgcttttac agcgtttgtt tttttaaata
     3121 catggcaaat gtatatgaaa tacttccaaa aagcaagcgt tttgctggca tttttttctg
     3181 aaaatgcaac aattaacatt attattcgtg cataaacagg atattatgtc cgaaaaatat
     3241 ccggagcagc aacaaacggc agaaactttt attctatttc atttcatttt aatccggtgc
     3301 aaactttacc taaagcattc ggggttttta caaagaaaaa cgcaactgaa tgattatcag
     3361 ctgcgtttta ttttttttgt ggtaccacca ggaatcgaac cggggacaca aggattttca
     3421 gtcctttgct ctaccaactg agctatggta cctcgtttgt tttgcgagtg caaatgtacg
     3481 gcattttttt gaattaccaa atacttcggc aaaaaatcct gaaaaattct ctgtggataa
     3541 tataacaaac ccgttactga cgttgggtga acccactatt taccggaata tgtatctaaa
     3601 tgtcagttaa ataacattta tactgtttat aggtaacggt tgtatttgga gataaaagat
     3661 ttttttttgt aaaaaagaat taactttgca attcatctta aataaaatac aatatctgca
     3721 aaaaaaatga caatatctac tgaacttttc ttgcttgtag catcagtttt gctgttcttt
     3781 agtttattga tagggaaaac cggacacaaa tacggtgttc caaccctgtt gttattcctc
     3841 atgataggta taatggcagg taccgacggt cttggtcttg agtttaattc tcccggaatt
     3901 actcagttta tcggtgtgat tgccctgaat attattttgt tctccggggg tatggatacc
     3961 aatttgtatg aaatcaaacc aattgttact cagggtctgg ttcttgctac tgtcggtgtt
     4021 ctgataaccg cacttctgac cggattattt attttctgga taaccaacag tatttttact
     4081 tccgtgtcct ttacgttgct cgaatcgctg cttttaggga gtatcatgtc atccaccgac
     4141 tctgcctccg tgttttccat cctgcgttcc aaggggttat cgctcaaaga aaatatccgt
     4201 ccgttgctcg agtttgagag tggaagcaac gatcccatgg catttatgct cactattgtt
     4261 ttaatccaac tgatactaac tcccgatttc agcacagggg aagcggtttt tatgtttttc
     4321 cgtcagttga tactgggcgc tattggaggt ctctttttgg gaaaattgtc tgtaaaaata
     4381 attaacagga taaatcttga caacgatgcc ttatattcag tgttgttgtt gactatcatg
     4441 ttttttctgt tcggatttac cagctttgtg ggtggaaacg gctatctggc tgtgtatgca
     4501 ggcggattgc tgattgggaa tcacaaattt gtacataaac gaagttcact gaagttcttc
     4561 gacggactga catggctttt tcaaattatc atgttcctca cactcggttt gctggtcaat
     4621 ccgggtgagt tgcttccgat tgccggagtg gctttgttga tcggcgtatt tatgttgctt
     4681 gtttcacgtc ctgcagcagt ttttatcagt ttgacaccat tccgtaaact tacctttaag
     4741 gccagaacgt atgtttcctg ggtgggactg agaggagctg tacccatcat tttcgccaca
     4801 tatccctgga ttgaaggggt gcctaaagca aaaatgttat ttaatatcgt cttttttatc
     4861 accatccttt cgctggtcat acagggaacc actgtaggaa caatggctca ttggatgaaa
     4921 ctatcgctgc cggtgccgaa aaaacgcaaa ctgagagagt tcgatgtgga attttcggac
     4981 gaaataaaat ctgccatgag tgaaatttcc atcaaggaag agtttcttgc caatggttcg
     5041 cgactgatgg acatcaatgt acctgacaaa accctggtgg caatggtgaa gcggaaaggc
     5101 aaatatttta ttccgcgggg aaataccgag ttgctcgtag gggacaggat actggtaatc
     5161 accgatgatg aaaaagccct ggaggaaacc taccagtcga tgaagggaaa attatccgaa
     5221 acgtctccgt tgtaaaaaaa tctaataatc aaacaataat aatagtatga aaaaatcagt
     5281 aatta
//
LOCUS       DAOPYB010000205         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_19994_length_5228_cov_21.582447, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000205 DAOPYB010000000
VERSION     DAOPYB010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19994_length_5228_cov_21.582447"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            597..1043
                     /locus_tag="PKH58_11780"
     CDS             597..1043
                     /locus_tag="PKH58_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89753.1"
                     /translation="MQNSSAQSIKQLDEVLNAMILADQNQGNADAARLKSLIYDVQPR
                     LYLHEKGCKKNIEKPSVCLTAEPSALELLYEANPLFEQVELITLNILTVRDMNFVLDL
                     ARLKSFSKLKYVQFLCNYDCNPATLMIKRPQEACSIIVFYQISLPE"
     gene            1074..>5228
                     /locus_tag="PKH58_11785"
     CDS             1074..>5228
                     /locus_tag="PKH58_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89754.1"
                     /translation="MNHVNFTQVITSTARRGLLVVAILFCNIFFVNAQVQKAFTQRTS
                     IYTPTKTIYNIKGDFTMIGNTNMTLEDYGDNILNSNNIMIKVDADSDPSTNNSSMATL
                     SFSTENGAIPECSNIIYAGLYWTARTNTYVTTEVKRSIKLKCPGESSYNTLTATVSDI
                     QYPGDNYMYAGYIEVTDYVRAHGIGEYWVADMALTTGNGGSTGYYGGWGMIVVYENSK
                     MNWRDVTIFDGYAYVEGNASVYYDLPITGFTTVQSGPVDMKLGLMAGEGDVGISGDYF
                     QIRKISDATWMSLNHGGNTTNNFYNSSIYTGGNTRNPNLLNNTGMDVSMFNIPNPSNT
                     VISNNQTSATFRYGSTQDTYIIFCIAMAVDAYIPYPEGLNVTQSIGGVPYTPGNPLVS
                     LPGDTLTFKLELRNKGTEAINNTRIVIPTPFATDFVSCSRTVNFVPLPTPNNLYFDPT
                     LGATGSIVWDFGTLPLPQDPDSVLAVLTYKLKVTENCVILKSQTCPPVISVNGTLSGT
                     GAISGVNFQNISFIQGYVMSGLCTGEPLVDPINININSTAYVDQYCQGTPVEREFVFC
                     NVGATIPVTSISGNFPAGCRFYNAYPVLPTTTEYNITNPFPATTGSHTYYAVPPGTTD
                     CYFVFIITVTNVYTTPTAGPVNYCLGAVAQALTATPSNISYLLFYYTSLSPSEIPQLS
                     ITPSTASVGTTTYYVAEGVSASCISPNRIPIVVTVSPPPAVLVSSNSPVCAGSNLYLY
                     ETGGQATTWNWTGPAGFTSTNDTAMIPGASVSNDGLYAVTVSNGLCSNSTSFDASVTQ
                     EPIVVTGCPDDITVCADTIFQSVLGAFVDWNIPDFSMNCMAALPGSSHNFVMGFELPE
                     VKWYCWEFNKVQRIGPSFVNLWQSVGTGDPYILTPSVYIEPALDVEMDIVVEPGRSFT
                     WSVFLVSGLTETFAGSVFVNATGHYTVNIPGSFDEGIYRLKFVFSGNGNNKCAVDNIY
                     FDGVLMDLGDCVGGIEFTVTGPIPGFYFCGDTNLVYTATYTPPVGTPITETCSFTVNV
                     ECVSAQVMTVNNTTCSLNNGSITISAQSNVSAHNFEYLINGGVWTAFGAGNSIVTISN
                     LPAATYQINIRELGHAGDCEILEPLTAVVGTTVDTVRPVISCPSNYSVNGCTTDAITG
                     LIYSESVVPISAGQFIAAGGSASDNCGVVYYSYQDSKTGDCPIVVSRVFTVRDLAGNT
                     KTCTQTIQIIHNTPPVVPSNGSAVVSCLSAAIAPTIPTVTDACGRPVNGILVSTVTLP
                     DPFICEGTRTYTYRFTDCAGLSADWVYIYTIERQDFTMPANPAPQIISCAADLAEPTL
                     PVITDNCNNLLTPTGPIISTIPACEGDVTYTYTYTDCEGNHHDWVYTYTIERQDFTMP
                     ANPAPQI"
BASE COUNT         1444 a         1113 c         1092 g         1579 t
ORIGIN      
        1 caaatatgat atccaagctg aatatgaagt cgtctgtgga ccttataaaa tatgctataa
       61 gaaacaaact ctgcgatcta taacctgctc atacggcagc gcttggttgt tacgaaaatc
      121 ctatcataaa aagcctcttg ggaatctttc cttttttgtc ataccctgaa aagcagtaca
      181 attttttttg ttttattttt ttattatttt tttccggaca gcagtgatat tttccggatg
      241 gaaagtcaaa ggtttcgctt tgcatttttc ctgacttttt tacaacattc tgtagaaaaa
      301 gataagtgaa tcactaattt ctgttttaag aaatacacgt atggttttca gtagattact
      361 atttcatagc cggatggatg gctatatctt tatgtttatg aagtacaata tattatgaat
      421 taagaataaa ggttttaagt gcttcaaaaa cagtgtaata aaaatatcaa aaacaaaaaa
      481 atatgaaaaa ttttatgtgc ttattttaca gtcaaattgc ccgtattttt ttaacattca
      541 gttttagaaa atgcaggttt tttattttat tatttgccat tatttatgct ttttcaatgc
      601 agaattcttc tgctcaaagc ataaaacagc ttgatgaagt gttaaatgca atgattttag
      661 cagaccaaaa tcagggaaat gcggatgctg cacgtcttaa aagccttatt tatgacgtgc
      721 aaccacgcct ttatctccat gaaaaaggtt gcaagaaaaa tattgaaaag ccctctgttt
      781 gtcttactgc cgagccttca gcgctcgaat tgctttatga agctaatccg ttgtttgagc
      841 aggttgaatt gataacattg aatatcctta ccgtccggga tatgaatttt gttttggatt
      901 tggcccggct taaaagtttt tcaaaactta aatatgttca gtttctgtgc aactatgatt
      961 gcaatcctgc aaccctcatg attaaacgtc ctcaggaggc ctgcagtatt atagtatttt
     1021 atcagatatc cttacccgaa taatatgtat tacacatcca ataccccctt gccatgaacc
     1081 atgtaaattt tacccaggtt attacaagca ctgccagacg cggcttgttg gttgttgcaa
     1141 tattgttttg caacattttt tttgtaaatg cacaggtgca gaaagcattt acgcagcgaa
     1201 cctccatata tacacctact aagacaatat ataacatcaa aggcgatttt accatgattg
     1261 gtaataccaa tatgacgctg gaagactatg gggacaacat actcaatagt aacaatatca
     1321 tgataaaggt ggatgccgat agcgatccgt ccaccaacaa ttcctccatg gctaccttgt
     1381 ctttttcgac cgagaacgga gcaatacctg aatgttcaaa tattatctat gctggattat
     1441 actggactgc caggacaaac acctatgtaa ctacagaggt gaagcgtagt ataaaactga
     1501 aatgtccggg cgagagcagc tataatacat tgacagctac tgtttctgat attcaatatc
     1561 ctggcgataa ttatatgtat gccggctata tcgaagtaac cgattatgtg cgggctcacg
     1621 gcattggcga gtattgggtt gcggatatgg cgctgacaac aggaaatggt ggttccaccg
     1681 gatattatgg agggtgggga atgattgtgg tgtacgaaaa ctcaaaaatg aactggagag
     1741 atgttaccat tttcgacggt tatgcttatg tggagggaaa tgcatctgtt tattacgacc
     1801 tgcctattac cggttttact acagtgcaat cgggtccggt cgatatgaaa ttgggattga
     1861 tggcgggcga aggagacgtg ggtatttcgg gagattattt ccagatccgg aaaattagcg
     1921 atgctacatg gatgagcctc aaccatggcg gaaataccac caacaacttc tataacagtt
     1981 ctatctatac aggtggcaat acccgtaacc cgaatctttt aaacaataca gggatggatg
     2041 tgagtatgtt caacatccct aatcccagca atacggtgat aagcaataat cagacttctg
     2101 caaccttcag gtacggatca actcaggaca cctatattat attttgtatt gctatggctg
     2161 tggatgccta tattccttat ccggaaggct taaatgttac gcaatccatt ggaggcgtac
     2221 catacacacc gggcaatccg ctggtatccc tgccgggcga tacactcacg tttaaactgg
     2281 aattgcgcaa caagggaacc gaagcgatca acaatactcg gattgtaata ccaactccct
     2341 ttgccacgga ttttgtgagt tgttctcgga cagtcaattt tgtgccactt cccactccta
     2401 ataacctgta ttttgatccg actttaggag ccactggatc tatcgtatgg gatttcggga
     2461 cactgccact cccgcaggat cctgattctg ttttggcggt attgacttat aaactaaagg
     2521 taactgaaaa ttgtgtcatt ttaaaaagcc aaacttgccc gcctgtgatc agtgtcaatg
     2581 gaaccttgag cggtacgggc gccatcagcg gcgtgaattt ccaaaatatc agctttattc
     2641 agggttacgt gatgagcgga ttatgcaccg gagaacctct tgtagatcct attaatatta
     2701 atattaattc aacagcctat gtggaccaat attgtcaggg aacaccggtg gaaagagaat
     2761 tcgtattttg caatgttgga gccaccatcc ctgttacaag tatttcaggg aattttcctg
     2821 cgggttgtcg tttttacaat gcataccctg tgttgccaac aacaactgaa tacaatatta
     2881 ccaacccgtt tccggcaaca actggttctc atacatatta tgcggtgcct ccgggtacta
     2941 ccgattgcta ttttgtgttt atcattacag ttacaaatgt ctatactact ccgacagcag
     3001 gtccggtaaa ctactgcctg ggggctgttg cccaagctct gacggctact ccttccaata
     3061 tctcatacct tttattttat tacacatcac tttctccctc tgaaatacct cagttatcta
     3121 ttacgccatc tactgcctct gtcggaacaa caacctacta tgttgcagaa ggtgtttcag
     3181 cttcgtgtat aagccccaac agaattccaa tagtagttac tgtgagtcct ccgcctgctg
     3241 ttttagtttc gagtaattca cctgtttgtg ccgggagtaa cctgtattta tatgaaacag
     3301 gaggtcaggc cacgacatgg aattggactg gccctgcagg gtttacctca accaatgata
     3361 cggctatgat accgggtgct agtgtttcca atgatggttt atatgctgtg acagtcagca
     3421 atggcctgtg ttccaacagt acttcattcg atgcttctgt tactcaagaa ccgatagttg
     3481 ttactggctg cccggatgat attactgttt gcgcagatac tatattccaa tcagtgctgg
     3541 gggcttttgt tgactggaac atcccggatt tcagcatgaa ctgcatggct gcattgccgg
     3601 gaagttcgca taactttgta atggggtttg aactgccgga agtcaaatgg tattgctggg
     3661 aattcaacaa agtccaaaga attggtccgt cgtttgtgaa cctatggcaa tccgtaggta
     3721 ccggtgaccc ttatatttta acaccatcgg tatatatcga accggcactg gatgtggaaa
     3781 tggatattgt tgtcgaacct ggccgcagtt ttacctggtc ggtatttctg gtcagcggat
     3841 taacagaaac ttttgccggc agtgtatttg ttaatgcgac ggggcattat accgtaaata
     3901 tccccggaag ttttgatgag ggtatttaca ggttgaaatt cgtattttcc ggcaatggaa
     3961 acaacaaatg tgcggttgat aacatatatt tcgatggtgt tttgatggat cttggcgatt
     4021 gtgtgggtgg aatagaattt actgttaccg ggcctattcc cgggttttat ttctgcggag
     4081 atactaattt agtttacacg gctacctata caccgcctgt tgggacaccg attacagaaa
     4141 catgcagttt tacagtcaat gtggagtgtg tctctgctca ggtaatgact gttaacaata
     4201 caacctgttc gttgaataat ggctccataa cgatttctgc ccagtctaat gtttcagcgc
     4261 ataacttcga atacctgatt aatggaggtg tctggacagc ctttggtgca ggcaattcta
     4321 tcgtgacaat ttcaaatctt cccgcagcga cctatcagat aaatatcagg gaacttggcc
     4381 atgctggtga ttgcgaaatc cttgaacctc tgacggctgt tgtcggcacc actgttgata
     4441 ctgtgcgtcc ggtgatttca tgtccatcaa actattctgt taatggttgc actaccgatg
     4501 caataacagg tttgatctac tccgaatccg tagtgcctat ttcagccgga caatttatcg
     4561 cagcaggagg aagcgcttca gataactgcg gggttgtata ctattcatat caggattcta
     4621 aaaccgggga ctgtcctatc gttgtttcac gtgtttttac agtcagagat ttagccggaa
     4681 acacaaaaac gtgcacacag actattcaga taattcataa tacaccacct gttgtaccat
     4741 ctaatggctc tgctgtagtt tcctgcctct ctgctgcaat agcaccaacc atacctacgg
     4801 taacagatgc ctgtggaagg cctgtaaatg gaatcttagt ttccacagtt acattacctg
     4861 atccatttat ctgtgaggga accagaacat atacttatag gtttaccgat tgtgccggtt
     4921 taagtgccga ctgggtttac atctacacca tcgaacgcca agactttacc atgcccgcta
     4981 accctgcacc acagatcatt tcctgtgccg ccgatcttgc cgaaccgact ttacctgtca
     5041 tcaccgataa ttgcaacaat ttgctgactc caacaggccc gatcatcagc acgataccgg
     5101 cctgtgaggg agatgtaact tatacataca cgtatactga ttgcgagggc aatcaccacg
     5161 actgggttta cacctacacc atcgaacgcc aagactttac catgcccgct aaccctgcac
     5221 cacagatc
//
LOCUS       DAOPYB010000206         5204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_20120_length_5204_cov_10.468440, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000206 DAOPYB010000000
VERSION     DAOPYB010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20120_length_5204_cov_10.468440"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..639)
                     /locus_tag="PKH58_11790"
     CDS             complement(55..639)
                     /locus_tag="PKH58_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89755.1"
                     /translation="MEERPLNEKESLELITRMIKNTHQKLEEGNGIPFLVWGYTSIIV
                     SLLVWYQLNSTQDYQWNFLWFLIPLIGFPVQMFLQRKKEKGIKTYLEDIIKYVWTVIG
                     VAGFVVSMGAMFYWKLPILFIIIVLMATGTAITGLIIQFKPIIISGFISILLSFGCLI
                     FSNSYSILIFALVFLLMMVIPGHILNFKNRKRHV"
     gene            896..3364
                     /locus_tag="PKH58_11795"
     CDS             896..3364
                     /locus_tag="PKH58_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNX89756.1"
                     /translation="MKKYLLFLLLHLFTITVFANVTIYGRIIDANTQKPIEFVTVSVY
                     NPKTGKNVTGTLTNQDGVFNIPSVVNGDYELRASVVGYNSFSKPVKVNGETVALGNIP
                     LTEDAKTLKEVQVVGQGSQMRFDIDKKVFSVDQNIAAAGGSATDVLQNIPSVNVDNEG
                     NVSLRNNESVEVWINGKPSGLTAENRAQVLQQLPAESIESIEVMTNPSAKFKPEGTAG
                     IINLVMKKNRKAGYYGSLSAGSMFTAGSAPGINAGASINYSSSKIDAFANIGHRQMNM
                     TGDGFTDRKNFYNGDTTLLNQTSVNERSFGGTFFRAGLDYHLNAKNTLGISGFGHSGN
                     PTGTSTLNYLLKDQSPDLILRDYSRKNTDDNAHRGYNLNLNHKYDIDDNGSNIITNLS
                     YSGHSMNSNEQFVQTDHLNALNNLDITQTADNSNKEIEAKTDFTKKFGENTRLELGWN
                     STWQNRVSLAGGFDNANLIDILSYYNKFDYKEQVHAAYATYGTRFDNFSLQAGLRGEY
                     LRKQITSTSKNISTSATEINRLDPQSYFELFPSLYMSYTLPKNNELQLNYTRRVNRPR
                     GRQINSFRDYSDATTISYGNPALLPEFASAFELNYVKNWDNHTLSASMYYRFTDDVIQ
                     NVNFMRNNVMESTFMNLTKQQNTGLELVAKNRLFKIVNLTSSVNLYYAKLHEATYQDP
                     FNSAVNVKIPGNENFSWDTRVIANLMLSRTFSAQVTGMYSAPQVMAQGERAEDFSVDL
                     GLRKTFLDKKFSLNVMVRDLLNSRKMGSTSWGDGFYQVSENRFSGRMIGFTVTYNFGN
                     MKPKPSGKKNGNTGESVDEGMDME"
     gene            complement(3812..4894)
                     /locus_tag="PKH58_11800"
     CDS             complement(3812..4894)
                     /locus_tag="PKH58_11800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNX89757.1"
                     /translation="MKKIAEQFQLKSEIGTIRPLKIGFINDSFIVESKDPNNEWYFLQ
                     KINHNIFKNVAGLQQNISLVTDHLRKKLSERGVDNLDRKVLQIIPAKDGKLFYEDTDG
                     NYWRVYTHIKNTKSYDVINPELAYKAGVAFGDFQAMLSDIPHDDLIETIPNFHNMEFR
                     LEEFREAVKKNAAGRLEKTQWMVDEIEKRADEMCVPERLHREGKLPKRINHCDTKVNN
                     MLFDDNDEPICIVDLDTVMPGFVLSDFGDFMRTAANTGAEDDVNPENIGVNFEIFKAY
                     TKGYLEKATFLTPSELGNLALGVKLLSYMQTVRFFTDYLNGDTYYKIQHGEHNWQRTI
                     AQFELLQSQEKNYDWMQQVVFEFASK"
BASE COUNT         1670 a          947 c          969 g         1618 t
ORIGIN      
        1 agaatggaca tgacggccag ccggagttca gaatgtagca gcggatcgag ttccttaaac
       61 atgcctttta cgatttttga agtttaaaat atgcccgggg attaccatca tcagtaaaaa
      121 tacgagagca aagatcagta ttgaatagga attactaaaa atcagacacc caaaagacag
      181 caaaatgctt ataaatccgg atatgattat cggcttgaac tgaatgatca gacctgtaat
      241 tgccgttccg gtggccatca atacaattat aataaataaa atgggtaatt tccaataaaa
      301 catggcaccc attgaaacta caaaaccggc tacaccaatt actgtccaga catatttgat
      361 aatatcttct agatacgttt ttattccctt ttcttttttt ctttgcagaa acatctgaac
      421 aggaaatcca atgaggggta tcagaaacca cagaaaattc cattgataat cctgagtgct
      481 gtttaactga taccatacga gtaacgaaac aattatggag gtataacccc acaccagaaa
      541 gggaattcca tttccttctt caagtttctg atgcgtgttc ttaatcatgc gagtgataag
      601 ttctaaactt tctttctcgt tcaaaggtct ttcttccatt gattaaatta tttattgggt
      661 aatttgaatt gaaataacga tgcaaatata aatcggttta tataataaac caaatataaa
      721 tatcaaaatc tacatttatt taacgtaaat taattataga gttcttttca gtcaaaatat
      781 ccaataattt tcctcgttgt ttaaactttt agcttttcca tttgtcttat tgaaattatt
      841 cattttttgc tgtctgcagg tattgtggcg agacagttaa gtcaaaacga tttctatgaa
      901 aaagtattta ttatttttat tgcttcatct ttttactatt acagtttttg cgaatgtcac
      961 tatttacgga cgaatcattg atgcaaacac gcagaaacca attgaatttg taactgtttc
     1021 tgtatataac ccgaaaacag gtaagaatgt cacgggtaca ttaactaatc aagatggcgt
     1081 ttttaatata ccttctgttg taaatggaga ttatgaactg cgtgcaagtg ttgtaggata
     1141 caatagcttt tcaaagccgg taaaagttaa tggcgaaact gttgctttgg gaaatatacc
     1201 gcttacagaa gacgccaaaa cgctaaaaga agttcaggtt gtcggacaag gttcgcagat
     1261 gcgctttgat attgataaaa aagtcttcag tgtcgatcag aatattgctg cagccggtgg
     1321 ttcagctaca gatgtgttac aaaatatacc ctcggtaaat gttgataatg agggaaatgt
     1381 atctctacgt aacaatgaaa gcgtggaagt ctggataaat ggaaaaccat cgggacttac
     1441 tgccgagaac agggctcagg ttttacagca attgccagcc gaaagcatcg aatctattga
     1501 ggtcatgaca aatccttccg ctaaattcaa accagaggga acagcaggta ttattaatct
     1561 ggtaatgaaa aagaaccgca aggcgggtta ctatggaagc ctttcagccg gtagtatgtt
     1621 tactgcaggt tctgctcccg gtattaatgc cggagcaagc atcaattaca gcagcagtaa
     1681 aattgatgca tttgccaata ttggtcaccg acagatgaat atgaccggtg acggatttac
     1741 tgatcgtaag aatttttaca atggtgatac tactctgttg aatcagacaa gcgtcaacga
     1801 gcgctctttc gggggtacat ttttcagagc agggctggat tatcatctga acgctaaaaa
     1861 tactctcggg atttcgggtt tcgggcattc aggtaatcct accggcacat ctacactcaa
     1921 ttatctgctc aaagatcagt ctccggattt aatcctgaga gattacagca gaaaaaatac
     1981 agacgataac gctcaccgtg gatataacct gaatttaaat cacaagtacg atattgatga
     2041 taacggttcg aatattatca ccaatctgtc ctattcagga cattccatga acagcaacga
     2101 acagttcgtt cagacagatc atcttaatgc acttaataat ctggatataa cccagactgc
     2161 agataattct aacaaggaaa tagaggcaaa aacagacttt accaagaaat ttggcgaaaa
     2221 tacacgactg gaactggggt ggaacagcac ctggcaaaac agggtttcat tagccggcgg
     2281 gtttgacaat gcaaatctga ttgacattct aagttattat aacaaatttg attataagga
     2341 acaagttcat gctgcttatg caacatacgg aacgcgtttc gacaattttt ccttacaagc
     2401 aggacttcgt ggtgaatacc tccggaagca gattaccagt acatcaaaga atatcagtac
     2461 ttcagccaca gagatcaaca gactggatcc acaatcttat tttgagctgt ttccaagttt
     2521 gtatatgtca tatacattgc caaaaaacaa cgaactgcaa ttgaactata cccgtcgggt
     2581 gaatcgtccg cgtggccgcc agatcaattc attccgcgat tattccgatg ccactaccat
     2641 ctcatacggt aatccggctt tacttccgga atttgcctct gcatttgaac tcaactatgt
     2701 gaaaaactgg gataatcata cgctttcggc atccatgtat taccgattta ccgatgatgt
     2761 cattcagaat gtgaacttta tgagaaataa tgtaatggag agcacattta tgaatttgac
     2821 aaaacaacag aatacaggac ttgaactggt tgcaaaaaac agattattta aaatagtaaa
     2881 tctgacatct tcggtcaatc tgtattatgc taaactccac gaagcaacct atcaggatcc
     2941 gtttaattcc gcagtaaatg taaaaatacc cggcaatgaa aatttttcgt gggatacacg
     3001 cgtgattgcc aacctgatgt tatcgagaac cttttctgct caggttactg gtatgtacag
     3061 cgccccgcag gtaatggctc agggtgagcg tgctgaggat ttttcagtag atttaggttt
     3121 aagaaaaacc tttttggaca agaaatttag tctcaatgtg atggttcgag atttgctcaa
     3181 ttccagaaaa atgggctcta ccagttgggg cgatggtttt taccaggttt ctgaaaatcg
     3241 cttcagtggc cgtatgattg gatttacggt tacttataat tttggaaata tgaaaccaaa
     3301 accttccggg aagaaaaatg gtaatacagg ggagtctgtt gatgaaggta tggacatgga
     3361 ataaaaaaca tataatcaac tgttattcaa cgcttcgtta aacacgaagc gttttttttg
     3421 cgataaattg aatatagtat tcataggaaa taacttaaaa gcttgtgttt atcccaaggc
     3481 tcaattatat attgtaatgg gaattgctta aagtgcattc tttgacgtat gattaatgtg
     3541 tactttatag catttaagaa attgtaagat gaacttaatg tagatagaat tgttctagta
     3601 atagattttc caacgcaaca actatttata attggtattc tttgtatctt tcatattaca
     3661 gaacagacaa ataaattcaa tcaacaaaga attaacgcaa gatttgaagt atttaatagt
     3721 tgtgcgtaaa aaatccggat taattgtgtg tgaattaaaa aaacggggca atgtgaattg
     3781 tcccgttctt taatattaga aaggtatttg attattttga agcaaattcg aatactactt
     3841 gctgcatcca gtcgtaattt ttttcctggc tttgcagcaa ctcaaattgc gcaattgtgc
     3901 gctgccagtt atgttctcca tgctgtattt tataataagt gtctccgttc agataatctg
     3961 taaagaaacg aactgtctgc atataactca gaagttttac acccagagca agatttccca
     4021 actctgatgg ggtcagaaaa gttgcttttt caagataacc cttagtgtat gctttgaaaa
     4081 tttcaaagtt aacaccgata ttttccgggt tcacatcatc ctctgcaccc gtattggctg
     4141 cggtacgcat aaaatcgcca aaatcagaaa gaacaaaccc cggcattact gtatccagat
     4201 ctacaataca gattggctca tcattatcgt cgaaaagcat attattaact ttcgtatcgc
     4261 aatggttgat gcgtttggga agtttacctt cgcggtgaag tcgttccgga acacacatct
     4321 catctgcgcg tttctcaatt tcatctacca tccattgagt tttttccaga cgaccagctg
     4381 cattcttctt tacagcttct ctgaattctt ccagccggaa ctccatgtta tgaaaattcg
     4441 gaatggtttc aatcaaatcg tcgtgtggaa tatccgataa catagcctga aagtcaccaa
     4501 atgccactcc ggctttgtaa gcaagttcag gattaatcac atcgtaactt tttgtattct
     4561 taatgtgagt atatacccgc cagtaattac catcagtatc ttcgtaaaaa agcttaccgt
     4621 ctttagccgg aatgatttga agtacctttc tgtcaaggtt atcaacacct ctttcactaa
     4681 gtttcttacg taaatgatcg gttaccaggc tgatgttttg ttgtaatccg gccacatttt
     4741 tgaaaatatt gtggttaatt ttctgaagga aataccattc attgttagga tctttactct
     4801 ctacaataaa tgaatcgtta ataaatccga ttttcaacgg gcgtatagtt cctatctcgg
     4861 attttaactg aaattgttcg gcaatttttt tcatgatact tattattaat agaatttaag
     4921 caacaaaaat aaggatttct aatgaaatga atgtcataat tatgttctaa aaaattattt
     4981 attaagacaa tcgtaccagc aaaaagacct gtgaaaagaa gaattttaca gctttcaata
     5041 ataactcagg tgtttcttga aaattcttga gaatttattt aacataaaac aggctgatat
     5101 atagttgctt gtgatgaaat atgtgtttta tagcatattt atttgtgatt ttatttgttc
     5161 actttaaaaa ctttgtttac ctttgcaccc gctttgagag agaa
//
LOCUS       DAOPYB010000207         5190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_20188_length_5190_cov_8.607790, whole genome shotgun sequence.
ACCESSION   DAOPYB010000207 DAOPYB010000000
VERSION     DAOPYB010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20188_length_5190_cov_8.607790"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKH58_11805"
     CDS             <1..516
                     /locus_tag="PKH58_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNX89758.1"
                     /translation="FAAIPLMLLWLNISCQIFLIGAEISYVSQNLRNFDYEVDTDNIS
                     NRYKLYLEFFVAYIIVKRFEQSKPPMTVEDIVSEYKLPIRLLNQILTVLTNSNIIIPV
                     KKDKIETAYQPAVDINQLTINKMYSKIDLTGSDLFLNSKNEEMDAFWQKMRELQSKTD
                     KLREDILLKDI"
     gene            781..4086
                     /gene="secA"
                     /locus_tag="PKH58_11810"
     CDS             781..4086
                     /gene="secA"
                     /locus_tag="PKH58_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNX89759.1"
                     /translation="MGFNDILKKIVGNKATRDMKEISPEVTRIKAVYEEIKNLSNDEL
                     RARTELLKATIQEAIASENARIEELKASIEETELHLREKIYNEIDKLEKEITEKISKV
                     LDEILPEAFSIMKDTARRLNENNEVIVTATQFDRDLAARYDFVTIQGDKAYYKNEWTA
                     GGNKIKWDMVHYDVQLFGGVVLHKGKIAEMATGEGKTLVATLPVFLNALTHRGVHVVT
                     VNDYLSKRDSEWMGPLYMFHGLSVDCIDKHQPNSDQRRQAYLADITFGTNNEFGFDYL
                     RDNMANSPMDLVQRKHIYAIVDEVDSVLIDDARTPLIISGPIPKGEDQLFEELCPKVE
                     KLVNAQKVLVTKYLADARALMKSEKEKEREEGTLALFRSYKGMPKNKPLIKYLSEQGV
                     KAAMMKTEEIYMEQNNRRMPEATDPLFFVIDEKNNSIELTDKGIDLITGNSDDPLLFV
                     LPNVGEEMAELEKMTGLSADEKQNKKDEILQNYSVKSERVHTINQLLKAYTLFERDVE
                     YVVLENKVKIVDEQTGRIMEGRRYSDGLHQAIEAKERVQVEAATQTFATITLQNYFRM
                     YLKLAGMTGTAETEAGELWNIYKLDVVVIPTNRPIVRNDMEDRVYKTKREKYTAVIEE
                     IVKLVEAGRPVLVGTTSVEISELLSRMLTSRRINHNVLNAKLHQREAEIVAQAGQKGT
                     VTIATNMAGRGTDIKLTPEVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSVF
                     FVSLEDDLMRLFGSERISGVMDRLGFEEGEVIEHSMISKSIERAQKKVEENNFGIRKR
                     LLEYDDVMNSQREVIYAKRRHALMGERIGVDISNMIFDGAELIVENAKNSEDFEEFET
                     ELMKIYAMDSPIQEDELKGIRHKELTEKVVDAARQAFKRKTEKMASVAYPVIKDIYEN
                     KGNQYESILIPITDGKRVFNITSNLETAYHTEGKEVIKDFEKQTLLYVIDDEWKEHLR
                     QLDELRQSVQNASYEQKDPLLIYKLESFGLFKEMVDNINRKVLAILMRGQIPIREPEQ
                     VRQGEMQHRQDYSKYRTQKDDAGRNVPSSDPTKQDTREQQKPEPIRVEKKVGRNDPCP
                     CGSGKKYKNCHGA"
     gene            4124..5014
                     /gene="lgt"
                     /locus_tag="PKH58_11815"
     CDS             4124..5014
                     /gene="lgt"
                     /locus_tag="PKH58_11815"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNX89760.1"
                     /translation="MLLNYITWNVKPELFSVGELTVRWYGFLWAVGIFLALIIVQKLY
                     KHEKLPETWIDKQFLYVVIGTIVGARLGHCFFYEWHLLPQPVEFLGITFKYGNPYLTQ
                     PWKLLYIWQGGLASHGGAIGILIAMYLYHKNVTHRGFIWGLDRLVIGAAVTGLCIRLG
                     NLMNSEIYGSATTLPWGFIFVRDGQTEPMHPTQIYEMLYCLATFLVCWWLYWKKEAYK
                     RTGLIFGVFLLGIFGTRFLLEFIKLNQEAFEGGMILNMGQILSIPFIAWGVWLIYDSK
                     NHIQPEEVKPPAANKPNRKK"
BASE COUNT         1661 a         1084 c         1170 g         1275 t
ORIGIN      
        1 tttgcagcca ttccgttgat gcttttatgg ttgaatatct cctgtcagat attcctgatt
       61 ggcgcggaga tctcgtatgt gtctcagaat ctgcgtaact tcgactatga ggtagatacc
      121 gataatatca gcaaccggta caaattatat cttgaatttt ttgtggcata tatcattgtc
      181 aagcgctttg aacaaagtaa accacccatg accgttgaag atatagtcag tgaatataaa
      241 ctgcccatcc ggctgttgaa ccagattctg actgttctga ccaattcgaa catcattatt
      301 ccggttaaaa aagataaaat cgaaactgcg taccaacccg ctgtagatat caatcaactg
      361 acaataaata agatgtactc taaaatagat ctgacaggtt cagatctttt cctgaattcg
      421 aaaaacgagg aaatggatgc attctggcag aaaatgcgtg aactgcaatc caaaacagac
      481 aaacttcgtg aagatattct gttaaaggat atttaattat accttacctg atattctctt
      541 ctctaaatat tttgaaagat cataaaatac taaacacttt ttacggggat attacactgt
      601 taaattgcaa gaaaaaacta tctttgcagg aatttgcaaa aaaaataaaa tcagtatttt
      661 tgaattaata aataatcgta taaaagaata ataagaaaat gacatctgtt cagatgttta
      721 caaagttata actgatttct ggacacttac aggttgattg aaatcaataa ataaaataat
      781 atgggattta atgatattct gaaaaaaata gtaggaaata aagctacccg cgatatgaaa
      841 gaaatatcgc ccgaagtgac aaggattaag gctgtttatg aagagataaa gaacctgagt
      901 aatgacgaac tgcgtgcacg taccgagctg ttgaaagcga caatacagga agcgatagcg
      961 tcagagaatg cacgtatcga agagctgaaa gccagtatcg aagaaaccga acttcatctg
     1021 cgtgaaaaaa tctacaatga gatcgataaa ctggaaaaag aaatcaccga aaagatttca
     1081 aaagtacttg acgaaatact tccggaagct ttctccatta tgaaagatac cgcccgccgt
     1141 ctgaatgaaa acaacgaagt gattgtcact gccactcagt tcgaccgcga tctggctgcc
     1201 agatacgact ttgtaaccat tcagggcgat aaggcttatt acaaaaacga atggactgca
     1261 ggaggcaaca agataaaatg ggacatggtg cattacgacg tgcaactctt cggaggggtg
     1321 gtactacaca agggaaaaat tgcagaaatg gcaaccggtg aaggtaaaac gctcgttgca
     1381 actcttcccg tattcctcaa tgcccttaca caccgcggcg ttcatgtggt aaccgtaaat
     1441 gattatctgt ccaaacgtga ctccgaatgg atgggcccgc tgtatatgtt ccatggctta
     1501 tcggttgact gtatcgacaa acatcagcca aactccgacc agcgccgtca ggcatacctg
     1561 gctgatatca ccttcggcac caacaacgaa ttcggttttg actacctgcg cgacaacatg
     1621 gccaacagtc cgatggatct tgtgcagcgt aaacatatat atgccattgt cgatgaggtt
     1681 gactccgttt taattgacga tgcacgtact ccgttgatta tttcaggacc gatacccaaa
     1741 ggcgaagacc agttatttga agaattgtgt ccgaaagtgg aaaaactggt gaacgcgcaa
     1801 aaggtactgg ttaccaaata cctggccgat gcccgtgcgc tgatgaaatc agaaaaagaa
     1861 aaagaacgtg aggaaggtac gctggcattg ttccgctcgt acaaagggat gcccaaaaac
     1921 aagcctctga tcaaatacct cagcgaacag ggtgtgaaag ctgccatgat gaaaaccgaa
     1981 gagatttaca tggagcaaaa caaccggaga atgcccgaag caaccgatcc gctgttcttt
     2041 gtcattgatg aaaaaaacaa ttcgatagaa ctaaccgata aaggtattga cctcattaca
     2101 ggaaattcgg atgatccgct cctgtttgta ctccccaacg tgggtgaaga aatggccgaa
     2161 ctggaaaaaa tgaccggact ttcggcggat gaaaaacaga ataaaaaaga tgaaatactt
     2221 cagaattatt ctgtcaaatc agaacgtgtt cataccatca atcagttact gaaagcatat
     2281 accttattcg aacgcgatgt tgagtacgta gttctcgaaa acaaggtaaa aatcgtagat
     2341 gagcaaaccg gccgtatcat ggaaggtcgt cggtattccg atggtttgca tcaggctatt
     2401 gaagccaagg aacgcgttca ggtcgaagct gccacacaga cttttgccac cattacgctt
     2461 cagaattatt tccgtatgta tctgaaactg gccggtatga ccggtacagc cgaaacggaa
     2521 gccggtgaat tgtggaatat ctacaaactt gacgttgtgg taattccaac caaccgcccc
     2581 attgtgcgga acgacatgga agaccgagtt tataaaacca aacgtgaaaa atacacagca
     2641 gtaattgaag aaattgtgaa actggtagaa gccggaaggc ctgttctggt gggtacaaca
     2701 tccgttgaaa tttcggaatt actcagccgc atgcttacca gccggaggat taatcataat
     2761 gtactgaatg ctaaactgca tcagcgtgaa gcagaaattg ttgctcaggc cggtcagaaa
     2821 ggcacggtta ccattgctac caatatggcc ggtcgtggta ccgacatcaa gcttaccccc
     2881 gaagtgaaag aggcaggcgg tttggcaatt atcggtaccg aacgtcacga aagccgccgc
     2941 gttgaccgtc agttgcgtgg tcgtgcaggt cgtcagggtg acccgggttc ttccgtattc
     3001 tttgtttcgc tcgaagatga tctgatgcgt ttattcggct cggaacgtat ctcgggcgtg
     3061 atggaccgtc ttggtttcga agaaggagaa gtgattgaac attccatgat ttcgaaatcc
     3121 attgagcgcg ctcagaaaaa ggtggaagaa aataacttcg gtatccgtaa gcgtctgctc
     3181 gaatacgacg acgtgatgaa ttcgcagcgt gaggtgattt atgcaaaacg ccgtcatgcc
     3241 ctgatgggtg aacgtatcgg tgtggatatt tcaaacatga ttttcgatgg tgctgaactg
     3301 attgtagaga atgctaagaa ttcggaagat ttcgaagaat tcgaaacgga actgatgaaa
     3361 atctacgcca tggattctcc gatacaggaa gacgaattga aaggcatccg tcacaaggaa
     3421 ctgaccgaaa aggtggttga tgccgcccgt caggctttca aacgcaagac agagaaaatg
     3481 gcttcggtag cttatcccgt tatcaaggat atttacgaaa acaagggaaa tcagtacgaa
     3541 agtattctca taccgattac cgacggaaaa cgtgtattca acatcacttc aaatctggaa
     3601 acagcctacc acaccgaagg caaggaagta attaaggatt tcgaaaaaca aacactcctg
     3661 tatgtgatcg atgacgaatg gaaagaacac ctccgtcagc tcgacgaact ccgccagtcg
     3721 gtacagaatg caagttacga gcaaaaggat ccgctgctta tctacaagct tgaatcattt
     3781 ggacttttca aagaaatggt cgataatatc aaccggaaag tgcttgccat acttatgcgt
     3841 ggtcagatac ctatccgcga acccgagcag gttcgtcagg gtgaaatgca gcaccgtcag
     3901 gattatagca aataccgtac tcagaaagat gatgctggca gaaatgtacc atcgtccgat
     3961 ccgacaaaac aggatacccg cgaacagcag aaaccggaac cgatccgtgt ggagaaaaaa
     4021 gtgggacgca acgatccatg tccctgcggc agcggtaaaa agtataaaaa ctgtcatggc
     4081 gcttaattca cggaactata gtatataaat aaaaccattc aatatgttat tgaattatat
     4141 aacctggaat gtaaagccgg aattattctc ggtgggagaa ctgacggttc gctggtacgg
     4201 atttttgtgg gctgtaggta tttttctggc gcttatcatt gttcagaaac tgtataagca
     4261 cgagaaatta cctgaaacct ggatagacaa acagtttttg tatgtggtga taggaactat
     4321 cgtcggagcg cggctgggac attgcttctt ctacgaatgg catttgctcc cgcagcctgt
     4381 cgaatttctg ggcatcacct tcaaatacgg aaatccctac ctgacacaac cctggaaact
     4441 gctgtacatc tggcagggag gtctggcaag tcacggaggc gctatcggta ttctgatagc
     4501 catgtacctg tatcacaaga atgtaacaca tcgcggattt atatgggggc tcgaccgact
     4561 tgtaatcggc gcagctgtta ccggattgtg tatccgactg ggtaatctga tgaattccga
     4621 gatttacgga agcgccacta ccctgccctg gggatttatt tttgtacgcg acggacaaac
     4681 cgagcccatg catcccacac agatatatga aatgctttac tgtctggcaa cattcctcgt
     4741 ttgctggtgg ctctactgga aaaaagaagc ttacaaacgt acaggtctga ttttcggagt
     4801 attcttactc ggcattttcg gaacccgttt tctccttgaa ttcatcaaac tcaatcagga
     4861 agcatttgaa ggcggaatga tactgaacat gggacaaatc ctgagcatac cgtttatcgc
     4921 atggggcgtc tggcttattt acgacagtaa aaatcatatt cagcccgaag aagtaaaacc
     4981 tcccgcagca aacaaaccaa acagaaaaaa atagttataa atcgaataag ccttgaaatc
     5041 aaggcttatt ttattaattt gtcagcatta tcatggacat aaatcatgta aaaccctaca
     5101 atcaggaaga agaaaaaacc aaacagctga accgcatgtt caacaccatt tcgcgtcagt
     5161 acgacaagtt caacgacatc atgagcatgg
//
LOCUS       DAOPYB010000208         5177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_20257_length_5177_cov_11.889887, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000208 DAOPYB010000000
VERSION     DAOPYB010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20257_length_5177_cov_11.889887"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /gene="guaA"
                     /locus_tag="PKH58_11820"
     CDS             <1..1320
                     /gene="guaA"
                     /locus_tag="PKH58_11820"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNX89761.1"
                     /translation="RIRGIFPVLGICYGAQFMAYSDGGKVEAAGSREYGRALLSEIDS
                     SDELLKGISAGSQIWMSHGDSITQMPEKFRKIASTSDVEMAAYRVENEKTWGVQFHPE
                     VFHTSDGMILLENFLDICQCRKEWSPTSFIESKVKELREQLGKDKVVLGISGGVDSSV
                     AAVLLNKAIGKNLTCIFVDNGLLRKDEFENVLKDYEHLGLNVIGVDAKDYFYNALKDV
                     KEPERKRKIIGSAFIDVFNQEAHKVKDVKWLAQGTIYPDVIESLSITGTVIKSHHNVG
                     GLPEKMDLKLCEPLRLLFKDEVRRVGSQLGMMSHLLKRHPFPGPGLAVRILGDITPEK
                     VRILQEADDIFISGLREWNLYDKVWQAGVILLPVQSVGVMGDERTYENAVALRAVTST
                     DAMTADWAQLPYDFLAKVSNEIINKVKGVNRVVYDISSKPPATIEWE"
     gene            1418..2188
                     /locus_tag="PKH58_11825"
     CDS             1418..2188
                     /locus_tag="PKH58_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNX89762.1"
                     /translation="MIDTHSHIYSEEFDSDRDEVILRAQHAGVTKIILPNVDSDTLPR
                     LLELEARYPDYCYAAIGLHPESVKEDYLKQLEIIKAELERRKYIAVGEIGIDLYWDKN
                     YQTEQIRAFQTQVEWAIELNLPVIIHVRNSHRETIDTLKHYAGSGLKGIFHCFGGTPE
                     EADEIFSLGDFKLGIGGVVTFKNSGLADSLMHIPLEKLVLETDSPYLTPAPFRGKRNE
                     SSYLRYVAVRLAEIYNVSQDEVDRITTESTCSVFSGLK"
     gene            complement(2252..2339)
                     /locus_tag="PKH58_11830"
     tRNA            complement(2252..2339)
                     /locus_tag="PKH58_11830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2303..2305),aa:Ser,seq:gga)
     gene            2521..3462
                     /locus_tag="PKH58_11835"
     CDS             2521..3462
                     /locus_tag="PKH58_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNX89763.1"
                     /translation="MSILLIVIAVFVLLIIFTGFCTVNQGYVAVTTIFGKYQRIMGPG
                     LNFKIPLIEMVHKRISIQNRSVELEFQAVTQDQANVYFKAMLLYSVLNQSEDVVKNVA
                     FKFVDDRNFMQALIRTIEGTIRSFVATKKQAEILGLRTEIILEVKKHLDDTLESWGYH
                     MIDIQLNDITFDEEIIKSMARVVASNNLKAAAENEGQALLITKTKSAEADGNAIKISA
                     LAEKEAAMQRGQGIALFRQEVAKGMAQAAQEMTVANLDASYLLFSMWTEAIKHFAETG
                     KGNVMFLDGSTTGMENTMKQMMAMMKLNEPIAVDKKK"
     gene            3715..>5177
                     /locus_tag="PKH58_11840"
     CDS             3715..>5177
                     /locus_tag="PKH58_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNX89764.1"
                     /translation="MKKFTEYSQLNLSDVNKKMLEYWNRNDLFHKSVETREGHPSFVF
                     YEGPPSANGMPGIHHVMARTIKDVFCRYKTMQGFQVKRKAGWDTHGLPVELGVEKSLG
                     ITKEEIGKTISVEEYNAACRTDVMKYTREWEDLTTQMGYWVDMNDPYITYDNRYIETL
                     WWLLKELYKKGLLYKGYTIQPYSPAAGTGLSSHELNQPGCYRDVKDTTCIAQFKVKSK
                     DNTYFLAWTTTPWTLPSNTALCVGLNIDYVLVKAANPYSKAENNYILAENLLTTVMGK
                     TEYEVIKKMKGADLVGEAYEQLIPWVNPGEGAFRVISGDFVTTEDGTGIVHIAPTFGA
                     DDDRVAKQTGIPPLMMLDKDGNRQPMVDKTGKFYTFDDLDKDFVEQYVNTALYSEFAG
                     KYVKNAYDIALGEADESLDIAICMMLKQQGLAFKIEKHIHSYPHCWRTDKPVLYYPLD
                     SWFIKSTACREEMIELNNTINWKPQSTGTGRFGKWLE"
BASE COUNT         1540 a         1060 c         1170 g         1407 t
ORIGIN      
        1 cggataagag gcatctttcc tgtgctgggt atctgttatg gcgctcagtt tatggcatat
       61 tccgatggtg gaaaagtgga agccgccgga agtcgcgaat acgggcgtgc tttactttcc
      121 gaaattgatt cgtccgacga gctgctgaaa ggtatttccg ccggttcgca aatctggatg
      181 tcgcatggtg acagcataac ccaaatgccc gaaaaattcc ggaaaatagc cagtaccagt
      241 gatgttgaaa tggcagcata ccgcgtagaa aacgagaaaa catggggcgt gcagtttcat
      301 cccgaagtgt tccatacctc cgatggtatg atcctgctcg aaaatttcct ggatatctgt
      361 cagtgccgaa aggaatggtc gcccacttca ttcatcgaga gtaaagtgaa agagctgaga
      421 gaacagttgg gaaaagacaa agttgtactc ggtatttcag gtggtgttga ttcatcggta
      481 gctgccgtgc tgctcaataa agcaatcgga aaaaacctta cctgtatatt tgtcgataac
      541 ggtttgcttc gcaaggacga atttgagaat gtactgaaag attatgaaca tctcggtctg
      601 aatgttatcg gtgttgatgc taaagattat ttctacaatg cgctgaagga tgtgaaagaa
      661 cctgaacgga aacgtaaaat cattggttca gcatttatcg atgtttttaa tcaggaagct
      721 cacaaagtaa aggacgtgaa atggctggct cagggcacta tttatcccga tgtaattgag
      781 tcgctgtcta ttaccggaac tgtcatcaag tcgcatcaca atgtgggagg acttccggaa
      841 aaaatggatt tgaaactttg tgagccgttg cggttgctgt ttaaagatga agttcgtcgg
      901 gtgggaagtc agcttggcat gatgagtcat ctcctcaagc gtcatccgtt ccccggaccg
      961 ggacttgctg tacgaattct gggcgatatt acccccgaaa aggtaagaat acttcaggaa
     1021 gctgatgata ttttcatcag cggacttcgc gagtggaatt tgtacgataa agtttggcag
     1081 gcaggagtga tattgctgcc tgtacaatct gtaggagtga tgggagatga gcgcacctat
     1141 gaaaatgctg tggcgcttcg ggctgtgact tcaaccgatg ctatgacagc cgactgggcg
     1201 cagctccctt acgattttct ggccaaagtt tccaacgaaa ttatcaataa agtgaaaggt
     1261 gtcaaccgag tagtttacga catcagttcc aaaccaccag caaccattga gtgggagtaa
     1321 taagcctcac cagaaattcc cgaagggaga gtcttaattt actttttaaa taaacgaatc
     1381 ataccataat tctcccttaa gtgggatagg gggcttaatg attgatactc attcacatat
     1441 ctattccgaa gaatttgatt ccgaccgcga tgaggtaatc ctgcgtgcac aacatgccgg
     1501 ggtgacaaaa ataattcttc ccaatgtcga ttcagatacc ctgccccgct tactggaact
     1561 ggaagcaaga tatccggact attgttatgc tgctattggc ttgcatcccg aaagcgtgaa
     1621 agaggattat ctaaaacagc ttgagataat caaagccgaa ctcgaacgaa gaaaatatat
     1681 tgcagtaggc gaaataggca ttgatctata ctgggataaa aattatcaga cggagcaaat
     1741 acgagcattt caaactcagg ttgaatgggc gattgagctc aacttacctg tcatcattca
     1801 tgtgaggaat tcccaccgcg aaacgataga tacattgaaa cactatgcag gcagcggatt
     1861 gaaaggaatt tttcattgct tcggaggaac tccggaagaa gccgacgaga tattcagtct
     1921 tggtgatttc aaactgggca taggtggggt ggtaacattc aaaaactccg gactggctga
     1981 cagtctgatg catattccgc tcgaaaagct ggtacttgag accgactcac cttatcttac
     2041 tcctgcaccg tttcgcggaa agcgaaacga aagttcttat ctcagatacg ttgcagtcag
     2101 gctggctgaa atctataacg tttcacagga tgaagtagat cgcattacga cagaaagtac
     2161 ctgttcggtt ttttcaggtc tgaaataaca aaacataaga ttactttaaa ataaaaaatg
     2221 catggctaaa aaccatgcat ttatcattct gcggagaaag cgggattcga acccgcgata
     2281 ccctttaggg gtatacacgc tttccaggcg tgcctcttca accactcgag catctctcct
     2341 tcggtatcgg actgcaaatg tataaaaata aaccgaaata tgaaaacctt tatccttttt
     2401 caaacctgaa taattttgca aaatcaattt taagtatttt gtgagaaatc tgacatagat
     2461 taaaaaaata catatctttg tgcgttaact ttttttattc acattataaa aaaatgcttt
     2521 atgtcaattc tattaattgt tatcgctgtt tttgttctcc tgataatctt tactggcttt
     2581 tgtacggtta atcagggtta tgtggctgtt acaactattt tcggaaagta tcagcgtatc
     2641 atgggtcctg gcctgaattt taaaattccg ttaattgaaa tggttcacaa gcgcatttct
     2701 atccagaatc gttctgttga acttgaattt caggctgtta cgcaggatca ggcaaatgtg
     2761 tatttcaaag caatgttact ctattcggta ctcaatcagt cggaagatgt ggtaaaaaat
     2821 gtcgccttca aatttgtcga cgaccgtaat tttatgcagg cgcttatccg taccatcgag
     2881 ggaactatca ggagctttgt tgccactaaa aaacaggctg aaattctggg attgcgcacc
     2941 gaaattatcc ttgaagtgaa gaaacacctc gatgacacac tcgaaagctg gggatatcac
     3001 atgattgaca ttcagcttaa tgatattact ttcgatgagg aaatcatcaa atcgatggct
     3061 cgcgttgttg cgtccaacaa cctcaaggcg gctgccgaaa acgaaggaca agcgttgctc
     3121 attaccaaaa ccaaatcggc tgaagcagat ggtaatgcca tcaaaatttc ggcattagcc
     3181 gaaaaagaag ctgccatgca acgcggtcag ggtattgctt tattccgtca ggaagtggct
     3241 aagggtatgg cgcaggctgc tcaggaaatg acagttgcca atctggatgc ttcgtacctg
     3301 cttttctcca tgtggacaga agccatcaaa cattttgccg aaacaggcaa gggcaatgtg
     3361 atgttcctcg atggatcaac taccggaatg gaaaatacca tgaaacaaat gatggccatg
     3421 atgaaattaa atgaacctat agccgttgat aagaaaaaat aatacacaaa actccatata
     3481 gaaagtgtac ttttggttcg aagattgaac caggtacact tttttttacc tgcatcccga
     3541 tttttcatat tcaattactc ctctctccct ggcattcatt ttttcgcgtt attctctttt
     3601 tttcatttcc atttattttt tgtaattttg caccctgatt ttttcagagc atcagcaaag
     3661 taagatgctt atattttgat atttactaac agcatttatc cacatatttt tcagatgaaa
     3721 aagtttacag aatattcaca gctgaatcta tccgacgtta ataaaaaaat gctcgaatac
     3781 tggaaccgta atgatttgtt tcacaagagc gtagaaaccc gcgaaggtca tccgtcattc
     3841 gtgttttatg aaggtccgcc ttctgccaac gggatgccgg gcattcatca tgtgatggca
     3901 cgtaccataa aagatgtatt ttgccggtat aaaaccatgc agggctttca ggtgaaacga
     3961 aaagccggct gggatactca cggactgcct gttgaactgg gagttgaaaa atcattggga
     4021 atcaccaagg aagaaatcgg aaaaaccatc agtgtggagg aatataatgc tgcctgccgg
     4081 accgatgtga tgaaatatac ccgcgaatgg gaagacctca ccacgcaaat gggttactgg
     4141 gtggatatga acgatccgta tattacctac gacaatcgct acattgaaac tctctggtgg
     4201 ctgctcaaag aactatacaa aaaaggattg ctttataaag gttacaccat tcaaccttac
     4261 tctcccgcag cgggtacagg gctgagttcg cacgaactca atcagccggg ctgttaccgc
     4321 gatgtgaagg atacaacctg tatcgcgcag tttaaggtga aaagtaaaga caatacctat
     4381 tttctagcct ggacaaccac tccctggact ttaccgagca atacggcgct ttgtgtggga
     4441 cttaatattg attacgtgct cgtaaaggct gcaaatccgt attccaaagc cgagaataat
     4501 tatatacttg ccgaaaatct gcttaccact gttatgggta aaacagagta cgaagtgatt
     4561 aaaaaaatga aaggtgccga tctggttgga gaagcttacg aacagttgat accctgggtt
     4621 aatccgggcg aaggagcttt ccgtgtcatt tcaggcgatt tcgtgacaac ggaggatggt
     4681 accggtatcg ttcacattgc gcccaccttt ggtgccgatg atgaccgcgt agctaaacaa
     4741 accggcattc cgcccctgat gatgcttgat aaggatggaa accgccagcc tatggtagat
     4801 aaaacgggta aattctatac cttcgatgac ctggataagg attttgttga acagtatgtc
     4861 aataccgctt tatacagcga atttgccgga aaatatgtaa aaaatgctta cgatattgct
     4921 ttgggcgaag ctgatgaatc gctggatatt gccatctgta tgatgctaaa acagcaggga
     4981 ctggctttca aaatcgaaaa acatattcac agctatcctc attgctggcg taccgacaaa
     5041 ccggtgctct actatccgct cgatagttgg ttcataaaat ccacagcctg tcgcgaggaa
     5101 atgattgaac tgaacaatac cattaactgg aaaccacagt caaccggaac aggtcgtttc
     5161 ggcaagtggc ttgaaaa
//
LOCUS       DAOPYB010000209         5159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_20358_length_5159_cov_10.385776, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000209 DAOPYB010000000
VERSION     DAOPYB010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20358_length_5159_cov_10.385776"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            658..3951
                     /locus_tag="PKH58_11845"
     CDS             658..3951
                     /locus_tag="PKH58_11845"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HNX89765.1"
                     /translation="MNKNYFLPCIYSFIIFFGLSVLTVGTSMAQKVHTSYLWHMDQPV
                     YWADKSKDKPDSKQFAEESHRLKMSGSNMYPGSAAAHPTNNLEEIFGKADRVQAYQYV
                     PRDAVNSIRDLPNAGAQLSISAGLLENMQSLGAKNQWGYTAGWMNAYKDAIAWKTGGN
                     LPRLDVVTFTYDHALSPLVSERTLIKQIKAHQYVSNKYYGYTSKGYWPAECAFSERII
                     KALVSCGVEWSVVPNSHLARTLADYVHPYNINGNSDEPNRADRVSTNGVNWFDGVIDG
                     RGSRLAAPYCYQAHKAQYIDPTTGTASVIDVVPMCNYISYIDGYGTHGTSEIATNIEP
                     FSNSSHPSLVLMAHDGDNAWGGGSSYYNESVTGFTHAAASAGYEPSTIQQFLKDHPVP
                     ADDVVHVEDGAWVNADSDWGHPQFINWLWPLYSKTNYRFDPDGWTEDARNWAVITATD
                     NYVTMAEDLEGGNLRIGYIADGGSDATNAEKAWHFYFGGLNSGFMYYGKAEDMEVKAS
                     MTGNIAIDYANRVISANSGTDNTAPSVFIPQRFPYNPGGYAFGPITGYKKTALSSDFH
                     VWTFAYDVSGLSSVTLKYRLDADGSNPVSSIQNETYAGGPEVEAWQETAMTRRAMAPD
                     PTTDAELNFFIQPAAKADLCYAEITGLKDKLVDYYVEAVDTKGNVYKSPIQHVYVGTG
                     DGQTPSGGDVSWTPASPNATETIIVSCKNATAASKLHWGVNTWSAPNAVYQPTGTVAI
                     SDKAVETPFQLVNGVWQVNLGPFNNPAQNVTSINFVINHGNNVWDNNNGSDYLITVKA
                     AQTNNPTGNNISKTLNISTAYTFTQSDFGFNSTVGNAFKGIKIVSLPGAGYLTSGGTA
                     ATVNQVVSNVGELVYTAAGTNASFSYRIVDSADLLSDAVYVASFTVTDPTSTAIRVSF
                     KRPADWGNTPVYLWAWTVSGNLFASWPGVTMTESSSGCFSYTFDKLVNNVNVIFSKNG
                     TPQSVDITGITQSTCFEYDAASGNKFTVKNTACETTSVSHIVVQEVSVYPQPAKSRFY
                     ITLPNTGNCKTYYLNLIDLSGRIVKTDHFIGQTTAVDCSSVKAGVYFVKIISGDGKEI
                     YTSKLIKSL"
BASE COUNT         1498 a         1199 c         1140 g         1322 t
ORIGIN      
        1 aagttgcatc agtcgttaat gcccatgtag agcttgcagt cgtctgcccc cacgctttca
       61 ctccactgcc cgtaagcagg agcgccagca agattaccgg caatagccgg aaaaaggagt
      121 ttgttttttt taaaaaagtc aaaattttca taagcgaatt aattattgtt ttatataata
      181 tcttcagttt caggcaataa aaaaaggcac atcaatccgg tgcgtatgaa ccggactgta
      241 tgcaaccacc atagtaatag aattccaaat atagaataaa tatttgaaaa acaaaacttt
      301 tgttgaaaaa aacgtatgaa gatactgtta caattcatat tgtatctttg taagtgtcgc
      361 cgtagatgct ttcagtcgcg tattgcctcg ttgaaaaaga atttcggtgg tgttgttttt
      421 ttagttgacg gcatcggact tactgatttt cagcgacaaa gaaacaatcg tttgcggaaa
      481 atatatcggt tttgctgata agcatctcgg tcggaaattg ttttcgtcct tatttttgtg
      541 aaatgtaaag tcccaggccc cctcccgatg catcgggata aattgacagg gagtaagacc
      601 agcgggaggc cgggattagg gacttttatt taatagaata gaaaacaagt atataccatg
      661 aataaaaact acttcttacc ctgcatttac tcatttatta tattttttgg gttgtcggta
      721 cttaccgtag gcacctcaat ggctcagaaa gtccacacct cctacctctg gcacatggat
      781 cagccggtgt actgggccga caaaagcaag gataaaccag acagtaaaca gtttgccgag
      841 gaatcgcacc ggctgaaaat gagcggaagc aatatgtatc cgggcagtgc agcggctcat
      901 cccaccaaca acctggagga aattttcggg aaagccgatc gagtgcaggc ttatcagtat
      961 gtgccgcgcg atgctgtcaa cagtatccgc gatctgccca atgcaggtgc acagctgagt
     1021 atttcggccg gactgctgga aaatatgcag tcgctgggag ccaaaaatca gtggggctac
     1081 acggcaggct ggatgaatgc ttacaaagat gccatcgctt ggaaaacggg cggcaatttg
     1141 ccccgcctgg atgtggtaac gtttacctac gaccacgccc tgtcgccgct ggtgagcgaa
     1201 cgcacactga taaaacaaat caaagcccat cagtatgttt ccaataaata ctacggatac
     1261 acttcgaaag gttactggcc tgcagaatgt gccttctctg aacgcatcat caaagcgctg
     1321 gtaagctgcg gtgtggaatg gtcggtcgtt cccaacagcc acctggcacg tacgctcgcc
     1381 gactatgtgc atccgtacaa catcaacggc aacagcgatg agcccaaccg tgccgaccgc
     1441 gtaagcacca acggtgtaaa ctggttcgac ggtgtgatcg atggtcgcgg aagccgtctg
     1501 gcagcgccct actgttatca ggcgcataaa gcacagtaca tcgacccgac aaccggaaca
     1561 gcaagcgtga tagatgtggt tcccatgtgt aactacatca gctacatcga cggatacggc
     1621 acacacggca cttccgaaat agccacaaac atcgaaccgt tcagcaacag cagtcacccc
     1681 tcgctggtac tgatggctca cgacggcgac aatgcctggg gcggtggcag cagctactac
     1741 aacgaatcgg ttaccggttt cacccacgct gcggcaagtg caggctacga accatccacc
     1801 atacagcagt ttctgaaaga tcatcccgtt ccggccgacg atgtggttca tgtcgaagac
     1861 ggagcgtggg tcaacgccga cagcgactgg ggccatccgc aatttatcaa ctggctgtgg
     1921 ccgctctatt ccaaaacaaa ctaccgtttc gatcccgacg gatggaccga agatgcccgc
     1981 aactgggcag taatcacagc taccgacaac tacgtaacga tggccgaaga cctggaaggg
     2041 ggaaacctgc gcatcggtta tatagccgac ggaggctccg atgccaccaa cgccgagaaa
     2101 gcctggcatt tctatttcgg cggtctcaac tcgggattta tgtattacgg caaagccgaa
     2161 gatatggaag tgaaagcctc tatgacaggg aatattgcca ttgattatgc caacagagta
     2221 atttctgcca attccggcac agataatact gcaccatcgg tatttatccc gcaacgcttc
     2281 ccgtacaatc ccggcggtta tgctttcgga cccattacgg gttacaaaaa aacagctttg
     2341 tccagcgatt ttcatgtctg gacatttgcc tacgacgtaa gcggactttc gtcggtcacg
     2401 ctgaaatacc ggctggatgc tgatggcagt aatcccgtca gcagcattca gaacgaaact
     2461 tatgcaggag gtcccgaagt ggaagcctgg caggaaactg ccatgacccg ccgtgcaatg
     2521 gctcccgacc ccaccaccga tgccgaactc aactttttta ttcagcctgc ggcaaaagct
     2581 gatttatgtt atgctgaaat taccggactg aaggacaaac tggtggatta ctatgtagaa
     2641 gctgtggaca ccaaaggcaa tgtgtataaa tcgcccattc agcatgttta cgtgggaacc
     2701 ggcgacggac aaacaccctc gggcggtgat gtaagctgga cacccgcttc acctaatgcg
     2761 actgaaacaa tcatcgtcag ctgtaaaaat gcaactgcag catccaaact gcactggggt
     2821 gtcaacacat ggtcagctcc caacgcggtg tatcagccaa caggaacggt tgctatttct
     2881 gataaagcag ttgaaacgcc atttcagctg gttaacggag tttggcaggt gaatcttggc
     2941 ccgttcaata accccgctca gaacgtaaca agcatcaatt ttgtaataaa tcacggcaac
     3001 aatgtgtggg acaacaacaa tggcagcgac tacctgatta ccgtcaaagc agcccaaacg
     3061 aacaacccca ccggcaacaa catcagtaaa acactgaata tcagcacagc ttacacgttt
     3121 actcaaagtg attttggttt taacagtaca gttggcaatg cgttcaaggg aataaaaata
     3181 gtcagtctgc ccggtgccgg atacctcaca tccggtggaa cagccgccac cgtcaatcag
     3241 gtggtaagta atgtcggtga actggtttat acggcagcag gtacaaacgc ttcattcagt
     3301 taccggatag ttgacagcgc cgacctgctg agcgatgcag tttatgttgc ctccttcact
     3361 gtgacggatc cgacaagtac agctatccgg gtatctttca aacgtcccgc cgactgggga
     3421 aacactccgg tatatctctg ggcctggact gtgtcaggca acttatttgc ttcctggccc
     3481 ggagttacga tgaccgaaag cagcagcggc tgtttttcct ataccttcga caaattagta
     3541 aacaatgtga atgtaatatt cagtaaaaat ggcacaccac agtcggtcga tataaccgga
     3601 ataactcagt ccacctgctt tgagtacgat gctgcgagcg gaaataagtt tactgtaaaa
     3661 aataccgcct gcgagaccac ttcagtaagc catatagtcg ttcaggaagt atccgtttac
     3721 ccgcaaccgg caaaatcgcg tttctatatt accctgccca ataccggcaa ctgtaaaaca
     3781 tattacctga acctgattga cctcagtggc agaatagtaa aaacggacca tttcatcggc
     3841 cagacaaccg ctgttgattg ttcatcggtg aaagcgggag tttacttcgt aaaaattatc
     3901 agcggagatg gaaaggaaat atatacctcg aaactgataa aaagtttata aggaaatccg
     3961 tttgattacc taaacttccg gaatttagat atcggagcta cgcccctaaa tactgccttt
     4021 tctgtgttta tctagcaact ggtcattccc taaaaacggg acaagttatg cggataatca
     4081 atttagataa tattcattgc attttaaaaa attcatattc cgctattacg gagcttatta
     4141 actaaaatag aaaatcagta gcctaaatat cgccactacg aagctatgaa taaatattac
     4201 cacataaata ttaacaagat gtcgctctgc cggagggtta aattactcat atacaacatt
     4261 ctttaccaca atgtttcgtt tacaaaatgg cagacacagt tccgcgaaaa caacactatg
     4321 atgccctctt tcagaattat gcacaaataa tacagaactt tgattcgatt ttttagaatt
     4381 tcgttcatat tttccaggac tttgatcgga ttttatggaa ctttgatcgg attttatgga
     4441 actttgttcg gattttatgg aactttgttc ggattttata ggattttgat caggatttat
     4501 ataatttcgt tctggttttt aaggtgtatg attattcttt ttagattttc gttcgtactt
     4561 tataggattc agatcggata attaatgatt ttgttttagg gctttatgat ttcgttcaag
     4621 cattattttc aataatccga aatattaaaa ttataacgtt atcctcaccg aataataatt
     4681 cctgtgtttt tacattcccg acttagcaga agtcagaaat gtgttgcaaa aatgaagaat
     4741 tctaaggtac gtctctgatg ggggtcgaat attgttgcga ttagatttca acaaatgtga
     4801 aattctgccg gaattcttca cttaaacaag aagtcaggtt gacgttaata atttaaatga
     4861 ctatccgtat aaagacctaa actataccaa taggggtttc acccctaaaa cccgacttac
     4921 ttttttgctt tgatacataa aaggaagaaa aaatcaatcc gccagctggc ggacgcttca
     4981 ctcgaaaaac ttgcgctcga caggctcaaa tcgcccatac tcccaccgtc gaaatacgac
     5041 ggtgctcaaa caaggacgat ttttaactgc ctgtctcact tgttttgcgg ctcaccagac
     5101 aaagtcggtc ggttgtttgc agataatcag tcattaggcc tttatgcgga taatcataa
//
LOCUS       DAOPYB010000210         5079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_20819_length_5079_cov_7.975119, whole genome shotgun sequence.
ACCESSION   DAOPYB010000210 DAOPYB010000000
VERSION     DAOPYB010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20819_length_5079_cov_7.975119"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2669
                     /locus_tag="PKH58_11850"
     CDS             <1..2669
                     /locus_tag="PKH58_11850"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX89766.1"
                     /translation="FTLRDVKPGKYILKITTIGYNDYNQNVTVANKDLLIGKISMQED
                     ARMLEQVNVTGTAVQVVVKNDTIEYNAQAFKTSQNAVVEDMLKKMPGVEVSSEGKITV
                     NGQEVKKVLVDGKKFFGDDIEMSTKNIPADMIDKVQVVDQKSEMAKLTGFEDNDTERV
                     INLTLKRDRKQGVFGNVQAGAGFDVNPDFRYDAGAFLNIMNGETRSTITAGANNTNTA
                     RSGRGREGFGGRSSGITATQNLGYNLNTPVNTKLILGGDASFNHSDQLQTSESNRENY
                     LQGLTYNNHSTSSSNRENYQGNVRLEAEWKPDSLTTVVFQPNIGYNRSFNSSNSNYLY
                     LTENDSTSWGKTNNSGNGTSVNGGLNIILSRKFAKPGRTFTTRLSTNVSQSNNDGFNN
                     SLKYTNDSTIVIDQRSENISNSYNMGLRMSFVEPLWKNQHFLETSVSLNSNFSNSTRN
                     LFDKDQQGNYTLLDDVYSNEFKNTFYSEALELNYRFIQKSYNVTLGMKAEPSQTYSTT
                     IYGSDPGVELSNEVINFAPNARFQYNFGKRQFARLDYSGRTSQPSISQMQPVKNNTDL
                     MNETVGNPTLNPQFQHNLRLMYSTYSSTKFSSFSFGLNGSVIKDDLTNNSIYDKTGKR
                     YIQTVNSKETPYNLNSFAMFSTPFWKKFNFSNNANFGVRQQYGYTSKNVSAELIDIEH
                     LQLGDLSSTIRYNAADNISLSYTHNIFDLSLRGGLGYSYSKNNFNTKSQETYDWTGAV
                     NLGVRPTSSLTLTTDLNYTTQQGYSSFNQSQWLWNASVELSAFKKKGVFSIKLYDILR
                     QRQNISQTVGDNYIQYSKTNSLPAYFLVGFTYKITKFKGATNQEMEDLQNMNRFGPDR
                     RMNRQGRGDSNSFHSPGGPPPFMGGGGPPPEM"
     gene            3324..4244
                     /locus_tag="PKH58_11855"
     CDS             3324..4244
                     /locus_tag="PKH58_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNX89767.1"
                     /translation="MKNVKIAILGLGGVGGYYGGKLAKRYEDTPNVEIYFIARGQHLQ
                     AIREHGIHIVNENEDFFAKPTLATSDAAEIGPVDFMIITTKSYDLKSSIEQIRPCIDR
                     DTVILPLLNGGDITERIREILPDNTVWSGYSYIVSRRTEPGVIRTSGPFLRLVFGYDG
                     GSDERLSAFETLLHEAEIDAHYSVNIRQSVWKKFFFISVSASLTSYLNVGFNELADER
                     RNLTADVAEEFLKVARAEGINFEGNPTEEIINRMEVLPAGSTASMHSDFKSGGNTELE
                     TLTGVVVRLAHQHNIPVPLYEKIYSELKKR"
     gene            complement(4292..4603)
                     /locus_tag="PKH58_11860"
     CDS             complement(4292..4603)
                     /locus_tag="PKH58_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNX89768.1"
                     /translation="MDNNENQNQLNIELSAEVAEGIYSNLAIISHSTSEFVLDFIRIM
                     PGTPKANVKSRIILTPEHAKRLLFALQDNINKYEQQNGKIKVPDNNMMPPIPMGFGGE
                     A"
     gene            4870..>5079
                     /locus_tag="PKH58_11865"
     CDS             4870..>5079
                     /locus_tag="PKH58_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89769.1"
                     /translation="MEKQVFQTDDKSRWIKFKWSLRVLAVFAAILVTALVIMLLIDKT
                     PAVPYKQSYKNIVSADKPYFQETKIS"
BASE COUNT         1566 a         1078 c         1118 g         1317 t
ORIGIN      
        1 ggtttaccct ccgtgatgtt aaaccgggta agtacatact taaaataaca accatcgggt
       61 acaacgatta taatcaaaat gtgacagtgg caaacaagga tttgctgata ggcaaaatat
      121 ccatgcagga agatgcccgc atgctcgaac aggtcaatgt taccggtaca gccgtacagg
      181 tggtggtgaa aaatgatacg atcgaataca acgctcaggc tttcaaaacc tcgcaaaacg
      241 cggtggtgga agatatgcta aaaaaaatgc ccggggttga ggtaagctct gagggcaaga
      301 taactgtcaa tggtcaggaa gtgaagaaag tgctggttga cggtaagaaa ttctttggcg
      361 atgacataga aatgtccacc aagaatatac cggctgatat gattgataaa gtgcaggtgg
      421 ttgatcagaa atcggaaatg gccaaactga ccggttttga ggataacgac accgaacgtg
      481 tgattaatct gaccctgaag cgtgaccgca agcagggagt tttcggcaat gttcaggccg
      541 gagccggctt cgatgtcaat ccggacttcc gttacgatgc cggcgcattc cttaatatta
      601 tgaatggtga aacccgttcg acgattacgg cgggagccaa caacaccaac acagcccgaa
      661 gcggacgggg tcgggaaggt ttcggaggaa gaagttcggg gattactgca acgcagaacc
      721 tgggttataa cctcaacaca cctgtcaaca ccaaactgat actcggcggc gatgcctcct
      781 ttaatcattc cgatcagttg caaacctctg aaagcaaccg cgaaaactat ctgcagggtc
      841 taacctataa caaccattcg acaagttctt cgaaccgcga aaactatcag ggaaatgtcc
      901 ggctggaagc tgaatggaaa cccgacagcc ttacaacggt ggttttccag ccaaatatcg
      961 gatacaacag atcattcaat agctccaatt caaattatct ttacctgacc gaaaacgaca
     1021 gcaccagctg gggaaaaacc aataacagtg gaaacggaac atcggttaac ggaggattga
     1081 atattattct tagccgtaaa ttcgccaagc ccggacgtac attcacaaca cgcctgagta
     1141 ccaatgtgtc gcagagcaat aacgacggtt ttaataattc tttgaaatat acaaatgatt
     1201 caaccatcgt cattgatcag cgaagcgaaa atatttctaa cagctacaac atgggtctgc
     1261 gtatgtcgtt tgtggaacca ctctggaaaa accagcactt tctggaaaca tccgtttcgc
     1321 tgaacagcaa cttcagcaat tcaacccgta acctcttcga caaagaccag cagggcaact
     1381 acaccctcct ggatgatgtg tacagcaacg agtttaagaa tacattctac agcgaagcgc
     1441 tggagttgaa ttaccggttt attcagaaaa gctataatgt cacgctgggt atgaaagccg
     1501 aaccttctca gacatacagc accaccatat acggaagtga tccgggcgtg gaactgagca
     1561 acgaagttat caattttgcc cccaacgcgc gttttcagta caatttcggt aaaagacagt
     1621 tcgcccgcct tgactattcg ggacgaacca gccagccttc catcagtcag atgcagccgg
     1681 ttaaaaacaa taccgacctg atgaacgaaa cagtgggtaa cccgacactt aatccgcaat
     1741 ttcagcataa cctgcgactg atgtactcca catacagtag cacgaagttt tcttcattca
     1801 gttttggact aaacgggagt gttatcaagg acgatctgac gaataacagt atctacgaca
     1861 aaaccggaaa gcgatatatt cagactgtca actcgaagga aacaccctac aacctgaatt
     1921 cgtttgccat gttcagcact ccgttctgga aaaagttcaa cttcagcaac aatgcgaatt
     1981 tcggtgttcg tcagcagtac ggatatacat caaagaatgt aagcgccgaa ctgattgata
     2041 ttgaacatct gcaactgggt gatttaagca gcaccatcag atacaatgca gccgacaaca
     2101 tttcactaag ctatactcac aatatatttg atctcagttt gcgcggcgga ttgggttatt
     2161 cttattccaa aaacaacttc aatacaaaat cacaggaaac ctacgactgg acaggtgctg
     2221 tcaatcttgg tgtccgcccc accagttctc ttacactcac taccgacctg aattatacca
     2281 cacagcaggg ttattcaagc tttaatcaaa gtcagtggct gtggaatgcg tcagttgagc
     2341 tgtcggcttt caaaaagaaa ggagtatttt caatcaagtt gtatgacatc ttgcgtcaga
     2401 ggcaaaacat ctcacagaca gtgggtgata actacattca gtacagcaag accaattcat
     2461 tgcctgctta tttcctggtt ggatttacat acaaaatcac caaattcaag ggtgccacaa
     2521 atcaggaaat ggaagatctg caaaatatga accgcttcgg tcccgatcgg agaatgaaca
     2581 gacaggggag aggcgattcg aattcatttc acagccccgg aggaccacct ccctttatgg
     2641 gcggaggcgg accacctcct gaaatgtaat gaaaaaaagc aactggaaag gaaacagttg
     2701 ctttttttgt aaatatatcc tgaatatctt gccaaaagtt gtaattttgt acactaaaat
     2761 tgggaaaagc cggatatttg aaaaggaagt ttgttgtgtc cgtgaaatac tgtgcaaacc
     2821 gtaagaacaa aatttgttaa tacagatacc gtattcccct tgctcctttg gagaaagggg
     2881 ctaggggatt gaggtctgag tttaatatta gttcttgtcc ggcaataatt tattgtcata
     2941 ccttttctat acgattgctg ttaacgggag ttcgatgtaa aaacacattt aatcaattta
     3001 agtacaattg tctgtttccc gtagggaatt aatatccata gaaaaaggtt tttaaataaa
     3061 ttaaattccc cgtaagggga taaatatatc aattccctac gggaaatgaa ccaatacaaa
     3121 aacacgcttt ctattgatat ttaagtccta acggactatt tcttatgttg aactcaggtt
     3181 ataaatttcc gccggaattt tgtaaagaaa tgaaaagaac agggataact tccatatata
     3241 aaaccgaaat ataaacaggc ttatcagcag aacaataaaa ctttttaata acaaacttat
     3301 ttgaacaata tataattata agtatgaaga acgtaaaaat agcaatactt ggactgggtg
     3361 gagtaggagg atactacggt ggaaaactgg caaaaagata tgaagatact cctaacgtgg
     3421 aaatatattt tattgcgcgt ggtcagcatc ttcaggccat cagggagcat ggcattcaca
     3481 ttgtcaacga aaatgaggat ttctttgcca aacccacact ggccacatca gatgccgcgg
     3541 aaattggccc ggtggatttt atgataatca ccaccaagag ttacgatctg aaatcgtcca
     3601 ttgaacagat acgtccgtgc attgacaggg atacggttat tctgccactg ctcaacggcg
     3661 gcgatatcac cgaacgaatt cgtgaaatac ttcccgataa taccgtttgg agtggatatt
     3721 cgtacattgt cagccgtcgg actgaacccg gagtgatacg cacgtcgggt ccgtttctcc
     3781 ggttggtgtt cgggtacgat ggcggcagcg acgaaagact ttcagctttc gaaaccttgc
     3841 tgcatgaagc ggagatagac gcgcattact cggtcaatat ccgccagagt gtgtggaaaa
     3901 agttcttttt tatttccgta tcagcctcgc tcacttcata tctgaatgta ggcttcaatg
     3961 aactagccga tgaacgcaga aatctgacgg cagatgttgc cgaagaattt ctgaaagttg
     4021 cccgtgctga gggaattaat tttgaaggca atcccactga agaaattata aacagaatgg
     4081 aggtactgcc ggccggatca accgcttcaa tgcacagtga tttcaaatcg ggcggcaaca
     4141 ccgaactcga aacactcaca ggcgtggtgg ttcgtctggc tcatcagcac aacatccctg
     4201 ttccacttta cgaaaagatc tacagcgagc tcaaaaaacg ttaaagggaa aacgtgaaac
     4261 gcaaaacccg aaacatgaaa cgcgaaacgt tttacgcctc tccgccaaat cccatcggaa
     4321 tgggtggcat catgttgtta tcagggactt tgattttacc gttttgttgt tcgtacttgt
     4381 ttatgttgtc ctgaagcgcg aaaagcaacc gtttggcatg ttcaggagtg aggatgatgc
     4441 gtgattttac attcgccttg ggtgtaccgg gcatgatgcg aatgaagtcg agtacgaatt
     4501 cggaagtgga gtgtgaaatg atggccagat tagagtatat tccttcggca acttcggctg
     4561 ataattcaat gttcagctga ttttggtttt cgttgttatc cataattgta tgttgttaat
     4621 gtttttcttg cgttcccgcg ttgtgtaagt tctgttaatg ttgttttttc tcttcggtat
     4681 tccgccttgc cgccatgtgc aacggcaagc tccgtaataa cacggtaaag atacgaaaat
     4741 atttatttcc cgcctgatat cataaaagca agcatgtttt aattttatgc aaatttaaac
     4801 ttgcatcatt aacatttaag cttacttaga agtggaatat gttatttttg cgcgttaaat
     4861 tctgtaatta tggaaaagca ggtttttcag actgacgaca aatcccgttg gataaaattt
     4921 aagtggtcgc tgagggtttt ggctgtattt gccgccattt tagtgacagc tctggtgata
     4981 atgctgctca ttgacaagac accggctgtt ccatacaaac aaagttacaa gaatatcgtt
     5041 tcggctgata aaccctattt tcaggaaaca aaaatctcg
//
LOCUS       DAOPYB010000211         4892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_21983_length_4892_cov_9.831507, whole genome shotgun sequence.
ACCESSION   DAOPYB010000211 DAOPYB010000000
VERSION     DAOPYB010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21983_length_4892_cov_9.831507"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..451)
                     /locus_tag="PKH58_11870"
     CDS             complement(68..451)
                     /locus_tag="PKH58_11870"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNX89770.1"
                     /translation="MESKKVEYNTADPRVYVHLKDARKELLLNPTCAESILWYYLRNK
                     KTGYKIRRQHVIDKYVVDFACLSKKLVIEIDGGIHLRQKEKDELRTLDLNNIGYKVIR
                     FSNDEVFDNAELVAQKIKKELNEEV"
     gene            1471..1851
                     /locus_tag="PKH58_11875"
     CDS             1471..1851
                     /locus_tag="PKH58_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89771.1"
                     /translation="MKTKNALFTAITIFLLTAGMGCKEKENIICTCGVPDPLVNLTWL
                     KEIKTNLENDPNIISSEIIMYSLEGQDYILVESHIDSAQDIPSTIYDCKGNEKYKCGG
                     NQLYDNCSIFFSKAQKKNLLWEKN"
     gene            1836..4472
                     /locus_tag="PKH58_11880"
     CDS             1836..4472
                     /locus_tag="PKH58_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNX89772.1"
                     /translation="MGKELKKERKSNVFFLFFILLPLFSYGQGLQRCPAFPDTLKMKQ
                     KGSYVLQCFLKGDEHLSIFTTTDGYPLVENKNGEFEYAIIDNFGNINSSSIKAKNIGD
                     RTIENRKYLERIDKKSILNLKKSSSNASFIDKNTANVANLLMNFPRTGTRNLLVLLID
                     FTDRPFQLANINFNNLMNQPTYNGTGSFRDYWTATSYGNLTINSTINGWYHAANNMAF
                     YGTNSNGNDTNPRLLVREAIDAAENAGTDFSIYDNDGDGKVDGIIVIHAGYGEEAGGG
                     QNTIWSHHWTLGNQSRTYDGVIIDDYAIVPELRDNEMWGINISNIGVMCHEFGHSLGL
                     PDLYDTNSSNGTSAGIGRWGLMGSGNWNNGGASPSHLCAWSKIFLGWSNPTLLSSPSS
                     ISLSNSVQNNNIYRINTSHSNEYFLLENRQSIGFDIGLPGAGLAIWHINTSKTTSTNI
                     SNNDVNADEDLKGVDLEEADGRNDLDVVVDNRGDNGDLFPGNTQNRTFRDNSNPNSQT
                     YSPIVSINKPIANIIEASGNVRFDFMGYNPITGPSTVCTQATYTIANFPTGAAVQWNV
                     SNNLQIISSTGNSVILQSIGTGAGWVEAVITIFGNNITLPRYNIQVSPAYALYAVSYP
                     DNCDEIVLKADVPDNTVITWTASPNILIDGYQSPYTKQGNTVSVYSTNNQEGSIQGLT
                     ASGCMVNEFIFTPCLDWSFTSNCEWTSPAPGEPLIASCSPAYSDAMKYEWYIHTQYLT
                     STDEPDLHTIEWPCIEGDLNVRAITPSGATQLTSACSFFPLCYSYSYFTLSPNPASSG
                     SVMVNLDETNLSKKQNTENTAIESQNKTYEIQLWNSFGLIKIVRTDMKKYQLDLNGIP
                     AGFYYVLVVKDGKTYRKQLIIK"
BASE COUNT         1577 a          863 c          917 g         1535 t
ORIGIN      
        1 cccgctttaa tatcgggata cttgtttcgg gaggttaggg gaggtctctt aaccaattca
       61 aattattcta aacttcctca ttcaattctt ttttaatctt ttgtgccact aattcagcat
      121 tatcaaaaac ttcatcatta ctgaaccgaa tgactttata tccgatatta tttaaatcca
      181 aagtacgtaa ttcgtctttt tctttttgcc ggagatgaat tccaccatca atttcaataa
      241 ccagtttttt tgacagacaa gcaaaatcaa ctacatattt atcaatcaca tgctgccgtc
      301 ttattttata acctgttttc ttatttctca ggtaatacca caaaatagac tctgcacatg
      361 tggggttaag taatagctct ttgcgggcat ctttcaaatg aacataaact cgcggatcgg
      421 ctgtattata ttccactttt ttgctttcca tctgtttttt tcgtaagttt cagcagtcgg
      481 acctccctcc ggctccctcc agggagggag agcactgatt gcaaaggatt attactttta
      541 tttttgtttg ttttatcttg cttgttatat tttaaaactc taatcaacct gcataactaa
      601 atcaactcat cattcccctc cttggagggg ttaggggagg tctcttaacc atgttttcaa
      661 taaaactata taaatgccaa taatccggat gttattgcca gacatagtcc ggcagtggtg
      721 actgagcttg tcgaagtcaa ctcccatata gcgaagtcgc ttcaccaaac atactgagcg
      781 ctaattgcag gttacaggga acgaggatag tttccaaact tcattgtgct aaaagtaagt
      841 ttcctgactt ccatattgca acagaatatg gaaagcaaag ctttccttct aactgcgacg
      901 taggcaggag ccatacgccg aacaaaagag tgtcagcgtt acactacggg gaacggggat
      961 taattaaatc ccggcgacca atgcacaaaa tctaaacatt taaagaacat atccggaagg
     1021 acgggagaaa gaatacttag cgagggcgtc actccatgtg acactctcgc ttatttgata
     1081 aacgtgtagg aaaatactac gaaccgatgc gttactttta tttttcacat tcttgatata
     1141 caaactatta agtctgtttt ttccgttact tttcttcagt atacgcttaa gtattgcggg
     1201 aaacttttct tttaaatagt tttgtggggt ttcgttagac attcagaaca ttcacctctg
     1261 ttgatcatag gttgtcatgg gtagttgtgt gcttcaatat ttcttaaatg acatttcatt
     1321 ttttctgcct cctctgcaac aaaacccctc tcttattcat catataagta tagtgatgtt
     1381 tcatgattta ttcattattt ttcaaattat atacgtaact ttataacgtt aatgctaatc
     1441 aattatacga accattataa ctaaatgcca atgaaaacaa aaaatgcttt atttacagca
     1501 attactatat ttctgctgac ggcaggaatg gggtgtaagg aaaaagaaaa tatcatttgt
     1561 acgtgtgggg tgccagatcc acttgttaat ttgacttggt taaaagaaat taagacaaat
     1621 ttagagaacg atccgaatat tatttcatca gaaataataa tgtatagttt ggaaggtcaa
     1681 gattatattt tagttgaatc acatattgat tctgctcaag atattccaag cacaatttat
     1741 gattgtaaag gaaacgagaa atataagtgt ggcggcaacc aactttatga taattgttca
     1801 atattttttt caaaagcaca aaaaaagaat ctattatggg aaaagaatta aaaaaagaga
     1861 gaaagagcaa tgtgtttttt ctgtttttca ttttgctacc cttattttct tatggacaag
     1921 gactacaaag atgtcctgct tttccagaca cgctaaagat gaagcaaaaa ggtagttatg
     1981 tacttcaatg ttttttaaaa ggagatgaac atttatctat ttttacaact acagatggct
     2041 atcctctggt tgaaaataaa aatggagaat ttgaatatgc aataattgat aattttggta
     2101 atatcaattc ttctagtatc aaagcaaaga acataggaga cagaacaatc gaaaatagaa
     2161 aatatttgga acgcattgac aaaaaatcaa tactaaattt aaaaaaatca tcttcaaatg
     2221 cttctttcat tgataaaaac acagccaatg tagccaattt gctaatgaat tttcctagaa
     2281 caggaacaag aaatctactg gtattattaa tagattttac agacagacca tttcaattag
     2341 caaatataaa ctttaataat cttatgaatc aaccaaccta caatggaaca gggagtttta
     2401 gagattattg gacagcaacc tcgtatggta atttaacgat aaacagtact atcaatggtt
     2461 ggtatcatgc cgcaaacaat atggcttttt atggcacaaa ttctaatggg aacgatacaa
     2521 atcctagatt gttggtccga gaagctattg atgcggctga gaatgcagga actgattttt
     2581 ctatttatga taatgatggc gatggtaaag ttgacggaat aattgtaatt catgctggat
     2641 atggcgaaga ggcaggagga ggtcaaaata ctatttggtc tcatcattgg acgcttggga
     2701 atcagagtag aacttacgat ggtgtaataa ttgacgatta tgcgatagtt cctgaattaa
     2761 gagataatga aatgtggggt ataaatatat caaatatagg tgtgatgtgt catgaatttg
     2821 gacattcttt gggcttacct gatttatatg atactaattc ttctaatgga acttcagcag
     2881 gaattggaag atggggatta atgggaagtg gcaattggaa taatggagga gcttctcctt
     2941 cacacttatg cgcatggagt aaaatttttc taggttggtc aaatcctaca ttattatctt
     3001 ctccatctag tatttcgttg tctaactctg tacaaaacaa caatatctat cgtataaaca
     3061 catctcattc gaatgaatac tttttgttag agaatagaca atctatcggt tttgatatag
     3121 gattaccagg tgcaggatta gctatttggc atattaatac atctaaaaca acatctacta
     3181 atattagtaa taatgacgtc aatgctgacg aggatctaaa aggagtagat ttagaagaag
     3241 cagacggtag gaatgattta gatgttgttg tagataatcg aggagataat ggagatttat
     3301 ttcctggaaa tactcaaaac agaaccttta gagataacag caatccaaat tctcaaacat
     3361 attctcctat tgtaagtatt aataaaccta tagcaaatat aattgaagca tcaggtaatg
     3421 ttagatttga ttttatggga tataatccta tcactggtcc ttctaccgtt tgcacccaag
     3481 ccacctacac tattgcaaac ttccctacag gagctgccgt gcaatggaat gtcagtaata
     3541 atctgcaaat aataagttct acgggtaact ccgttatatt gcaaagtatt ggtacaggcg
     3601 caggttgggt agaagcagta ataactattt ttggtaataa cataacgttg cccagataca
     3661 atatacaagt ttctcctgcc tatgctttat atgcggttag ttatccggac aactgcgatg
     3721 aaatagtact caaagccgat gtgcctgata atacggtaat aacctggaca gcgtcaccca
     3781 atatcctgat tgacggatat caatcgccct atacaaaaca aggaaatacg gtcagtgtat
     3841 attcaacaaa caatcaggaa ggcagcatac aagggctaac ggcaagcggc tgcatggtaa
     3901 atgaatttat atttactcct tgcttggact ggagttttac ttcgaattgt gagtggactt
     3961 caccagcgcc aggagaacct cttatagctt cttgcagtcc ggcttattct gatgccatga
     4021 aatatgaatg gtatattcat actcagtatc tcacatcaac agacgaaccc gatttgcata
     4081 ccatagaatg gccttgtatt gaaggtgatt tgaacgtgag agctataacg cccagtggag
     4141 caactcaatt aacttctgct tgttcttttt tcccgttatg ttatagttat tcttatttca
     4201 ctctttctcc aaatccggca tcttctggtt cggtaatggt aaatttggat gaaacaaatt
     4261 tatcaaaaaa gcaaaatact gagaatactg cgatcgaatc gcaaaataaa acctacgaaa
     4321 tacaactatg gaattcgttc ggattaataa aaatagtacg tacggatatg aagaaatatc
     4381 agcttgacct gaatggaatt cctgcaggat tctactatgt gcttgtcgta aaagatggaa
     4441 aaacttacag aaaacagctg ataataaaat aaaatgacga agtagtataa cgtgaagaga
     4501 aattgtgttt ttgcaaagaa atatgccccg ttggtgcggc gaagtttcca aacttcgtcg
     4561 cgctaaaagg aagtctccgg acttctacat tgaaacagaa tatggtaagc aaagccttct
     4621 ttcatcccag taaacaaaat accccgaaag ttactgataa cttccggggt atttgtttgt
     4681 atatccagcg cggattgcgt ccggcaggga tttactttat tcagccaata ttgtaatttc
     4741 ggctcctgtg gcaaaatcct gtccacgttg ttcgctaagg gcggtaaagc ggatgtaacg
     4801 ggctttggcg ggtttgtcaa agagaacttt cttttcagct ttgccgttgt cgaacttacc
     4861 acgcgctacg ggttcgcccc agtttttacc gt
//
LOCUS       DAOPYB010000212         4830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_22385_length_4830_cov_10.793089, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000212 DAOPYB010000000
VERSION     DAOPYB010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22385_length_4830_cov_10.793089"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..799)
                     /locus_tag="PKH58_11885"
     CDS             complement(254..799)
                     /locus_tag="PKH58_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX89773.1"
                     /translation="MQTQSLYQKAIKFAAAKHAAQNQTIPGTNLPYVVHLSNVAMEIL
                     IASFHSENFNLDFAIQVALLHDTIEDTSTTFGDLDTEFGLDIANAVFALTKNTDLPGE
                     VQMQDCIRKIKLLQPEVWAVKLADRITNLQPPPSHWNNERKIRYRDEAGFILSELRDG
                     NEYLTKRLEEKIEEYGGYVKD"
     gene            complement(811..1113)
                     /locus_tag="PKH58_11890"
     CDS             complement(811..1113)
                     /locus_tag="PKH58_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89774.1"
                     /translation="MELPEKLKTFIANQDWIFAKTYTATGPHEYIVQERVDKDLFQEL
                     ANHIDQFGYEDYFYEMKLIYFKYNELTYWHMGNIINRCLEEDTYRYRKAAGNLPEK"
     gene            complement(1232..2485)
                     /locus_tag="PKH58_11895"
     CDS             complement(1232..2485)
                     /locus_tag="PKH58_11895"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89775.1"
                     /translation="MKTYRHHITVFEHERIKLGQVFTGCEFDEPKLIAFQKYYGEKGV
                     PFFSLIHRGIQFNEHVGVIQVGDLLIEVLPKADNNRETADWRKMLIGMMRAVGSFDIR
                     VSSNSHLKLKSNTILDLYIEMFVNEVEYLLHSGLLKKYRKNESNVTALKGNIIFSKQI
                     QHNLTHQERFYVRHTTYDVEHTLHQILYVTIRFLRQINTNASLQSRIGALQLFFPEMP
                     PLKVTEATFDKLVYNPKSEAYRKAIEIARLLLLQYHPDVSKGKNHVLALMFDMNKLWE
                     QFVYVSLRKHADDYSIKPQSKKNFWQPEYGFKTSIIPDIVINKDTDRCVVMDTKWKNL
                     NGYNPSSSDLQQMFVYHQYFNAVKTALIYPGSGNAIKGTYFGADAKFNEQMQCYVIPI
                     DIQTGNVRAWQKKIADNIKGWLSGV"
     gene            complement(2482..>4830)
                     /locus_tag="PKH58_11900"
     CDS             complement(2482..>4830)
                     /locus_tag="PKH58_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX89776.1"
                     /translation="LIQQKGFTDEDYKWKLVNQLKGRPDVEAEDFKLEIKDLNPQENN
                     LVYQMAKGTSNRMAKLKPEEYRACFRSLFDESIDLKQRISNFMTEIVALYKSTEGENS
                     AHHDERTISAYLTFHNPEKYTFYKSTYYEEYCKLLDIKPKAVGEKYVHYLELINELID
                     NYILQDAELLDLVDEKLTGGEYYKDEKRLLLAQDILYRMLNLSIMTNYWIFQGNPSVF
                     DFETALKQNILTDWTVSAHKDKMKTGDKVIMWITGSKSGCYALGELISEPYEKETSPD
                     DHLWKSEDKSKLKVDIEITHNLVDCPIYKEKIKDVAELRNLKIGNQGTNFSATQQEFE
                     AILKLTNEATKNNQNNKSVNMIAFNQILYGPPGTGKTYNTINKAISIINPDFDLNQDR
                     KTVKQEFERLTNDGQIVFTTFHQSMSYEDFIEGIKPLEPSDEDIYLKYEIQPGIFKKI
                     CEAAKTPNQRDFSVAYENFIQDLSSMDKLDLKTPTGSSFSVTLNSRNNLSLYTGGNIE
                     GNGSLTKENLQIYFNEKEKIKYWKGYYEGVANYLMTKYKYSKTEKQRQKFVLIIDEIN
                     RGNVSQIFGELITLIEDDKRLGNSEALEVVLPYSKEKFGVPPNLYIIGTMNTADRSVE
                     ALDAALRRRFSFEEIRPKPEVITNRVKGDKGIVEGIDLGQVLETLNKRIELLVDKDHQ
                     IGHSYFLSVENLKELKSAFQNKIIPLLQEYFFGDYGKIGLVLGSGFVSVKEKENNIFA
                     DFNNLYDTSDLADREIYRIENPVKMDDGKFREAIKNLLNIKD"
BASE COUNT         1285 a          988 c          863 g         1694 t
ORIGIN      
        1 tcatttattt ttaactgcaa gggtacggca taccttttca caggatattg cggttgttaa
       61 aacggcattg tctgtaaaat tcagacttat cttttacgca tcttacggat ggaactgaaa
      121 tgtgtggtgt aaatatagtc tatctctgtt agattttttc ctgaccgaac agttttaata
      181 aatttgttag cttagcatca attttaatag tctacttaat agaagccaaa atatattttt
      241 tcaagtttta agtttaatct tttacatacc ccccatattc ctcaatcttc tcctccagcc
      301 gcttcgtcag atactcattg ccatccctga gttcgctcag gataaatcct gcttcatccc
      361 ggtacctgat ttttcgttcg ttgttccagt gagagggtgg gggctgaaga ttggttattc
      421 tatcagcgag ttttacagcc caaacttcag gttgcaataa tttgattttc cttatgcaat
      481 cctgcatttg tacctcaccg ggcagatcgg tgtttttggt aagtgcaaaa actgcattcg
      541 caatatcaag cccaaattca gtatccagat ccccaaaagt agtggatgtg tcttcgattg
      601 tatcgtgaag caaggcaacc tgaatggcaa aatccagatt aaaattctca gaatggaaag
      661 atgcaatcaa tatctccatt gccacattgc tcaaatgtac cacgtaagga aggtttgtgc
      721 ccggaatggt ttgattttgg gctgcgtgct ttgcagccgc gaattttatg gctttctggt
      781 agagtgattg ggtttgcatg taattttgtt ttatttttcc ggtaaatttc ctgctgcttt
      841 tctgtaacga taggtgtcct cttccaggca ccgattgatg atattgccca tgtgccagta
      901 ggtcaactca ttatatttga aatatatcag cttcatttca tagaaataat cttcgtaacc
      961 aaactgatct atgtgatttg caagctcctg aaacaagtct ttgtctactc gttcctgaac
     1021 aatatattca tgaggcccgg tagctgtata agtttttgca aaaatccaat cctgatttgc
     1081 tataaaagtc tttagttttt cgggtagttc catgttgaag tagattttat ttattcagcg
     1141 gcgatatcat atagtagaat acttttcata ataaaaaatc attgtatcga ctgaaaagat
     1201 ttcaacttct atattcaact gacggcatta attacacccc actcaaccat cctttaatat
     1261 tatcagcaat ttttttctgc catgccctga cattaccagt ttgtatgtct attggaatta
     1321 catagcattg catttgttcg ttgaattttg catcggcacc gaagtacgtg ccttttattg
     1381 cattgccgct tccggggtaa atcagtgctg ttttcactgc attgaaatac tgatgataca
     1441 caaacatctg ctgcaaatcg gatgaagagg gattgtaacc attcagattt ttccatttgg
     1501 tatccataac aacacagcgg tcggtatctt tattgatgac gatgtcagga ataatactgg
     1561 ttttgaagcc gtactcaggc tgccagaaat ttttcttgct ttgcggtttg atgctgtaat
     1621 catcggcgtg ttttcgcaaa ctcacataca caaattgctc ccagagttta ttcatgtcga
     1681 acatcagcgc cagcacatga ttttttcctt tactcacatc ggggtgatac tgaaggagca
     1741 gcaatcgggc aatttcaatg gccttacgat aagcctcgct cttgggatta taaaccagct
     1801 tgtcgaatgt tgcttcagtc actttcagcg ggggcatttc cgggaaaaac agctgcaaag
     1861 ctccaatccg gctttgaagc gaggcgtttg tgtttatttg ccggagaaac ctaatggtga
     1921 catataggat ttggtgcaaa gtgtgttcaa catcgtaggt agtgtgtcga acataaaaac
     1981 gctcctgatg cgtgaggttg tgctgtattt gcttactaaa aatgatgtta cctttcagtg
     2041 cagtcacatt actttcattc ttccggtatt ttttcagcag tccgctgtga agcaggtatt
     2101 caacttcgtt cacaaacatc tcgatgtaca aatcgagaat ggtgtttgat ttaagtttca
     2161 ggtgactgtt gctgctcacc cgaatatcaa aactgcctac agcccgcatc attccaataa
     2221 gcatttttcg ccaatcggca gtttccctat tgttgtcggc tttcggcagc acttctatca
     2281 gcaaatcgcc cacctgaatt accccgacat gttcgttaaa ctgaatgccc cgatggatga
     2341 gcgaaaaaaa gggaactcct ttttcgccgt agtatttctg gaacgcgatg agttttggtt
     2401 catcaaattc acagccggtg aatacctgcc cgagtttaat gcgctcgtgt tcaaatacgg
     2461 taatatgatg ccgataggtt ttcaatcttt aatattcaac aggttcttaa ttgcctctct
     2521 gaattttcca tcatccattt tcacgggatt ttcaatccgg tagatttcac gatctgccaa
     2581 atcggaagta tcgtacaggt tattaaaatc ggcaaatata ttgttttcct tttcttttac
     2641 cgacacaaat ccgcttccca gcaccagacc tattttgcca taatcgccaa aaaagtactc
     2701 ttgtagtaat gggattatct tgttttgaaa ggccgatttc agttctttca ggttttcaac
     2761 agacaaaaaa tagctgtgac caatctggtg gtctttgtca accaggagtt cgatgcgctt
     2821 gttgagggtt tcaagtacct gccccagatc aattccttcc actattccct tatctccctt
     2881 tacgcggtta gtgattactt caggtttcgg acgaatttcc tcgaaactga aacggcggcg
     2941 caaagcagca tccaatgctt ccacactgcg gtcggcagta ttcatcgtgc cgataatgta
     3001 aaggttgggt ggaactccga atttttcctt actataaggc aagacaactt ccagtgcttc
     3061 tgaatttcca agccgcttat catcttctat caaagtaatc agttcgccaa atatctgtga
     3121 aacgtttccc cggttgattt cgtcgatgat tagaacaaat ttttgtctct gtttttctgt
     3181 ttttgaatat ttatatttcg tcatcaaata attagcaact ccttcataat aacctttcca
     3241 gtattttatt ttttcttttt cgttgaaata tatttgtaaa ttttcttttg taagagaacc
     3301 attaccttca atatttccac ctgtataaag tgataagtta tttcttgaat taagtgttac
     3361 actaaaactg ctacctgtag gtgtttttaa gtctaactta tccatactgc ttaaatcctg
     3421 tatgaaattt tcataagcca cactaaaatc tcgttgattt ggagttttag ctgcttcaca
     3481 aatcttcttg aaaattccgg gttgaatttc atatttaaga tatatatctt cgtctgatgg
     3541 ttcaagaggt tttattcctt caataaaatc ctcgtaactc atgctttgat gaaatgtagt
     3601 aaatacaatt tgaccatcgt tcgtcagtct ttcaaactcc tgttttactg tttttctgtc
     3661 ctgatttaag tcaaaatccg gattgattat cgatatcgct ttattaatcg tattgtacgt
     3721 tttcccggtt cccggagggc cgtaaaggat ttgattgaat gcaatcatgt taacagattt
     3781 attattttga ttatttttcg tggcttcatt cgttaatttt aatattgctt cgaattcctg
     3841 ttgtgtagcg ctaaagttag ttccctgatt gccaattttc aagtttctga gttctgcaac
     3901 atcttttatt ttttccttat aaattggaca atcaacaagg ttgtgagtta tctcaatatc
     3961 aactttcaat ttacttttat cttctgattt ccacaggtgg tcgtctggtg aagtctcctt
     4021 ttcgtaaggt tctgaaatta attctccaag agcataacag ccggacttac taccagttat
     4081 ccacattatt accttgtccc ctgttttcat tttatcctta tgagccgaaa ctgtccaatc
     4141 ggtaagtata ttttgtttta atgcagtttc aaaatcgaaa acacttggat ttccctgaaa
     4201 aatccaataa tttgtcataa tgcttaaatt aagcattcga tacaaaatat cctgtgccaa
     4261 taacaatctt ttctcatcct tataatactc tccgccagtg agcttctcat caaccaaatc
     4321 aagcagctct gcatcttgca aaatgtaatt atcaatcagt tcattgatta attccagata
     4381 atgcacatat ttttcaccaa cggcttttgg ctttatatcc agcagcttac aatactcctc
     4441 ataatacgta cttttataaa aagtgtattt ttcaggattg tgaaaagtaa gataagccga
     4501 tatggttctt tcatcgtgat gggctgaatt ttcaccttca gtagacttgt agagggcaac
     4561 tatttcggtc ataaagttac tgatacgctg ttttaagtca atcgattcat cgaaaagtga
     4621 acgaaaacag gctctgtatt cttcaggttt taatttggcc attctattgg aagtaccttt
     4681 ggccatctga tataccagat tgttttcttg cgggtttaag tccttaattt ccaacttaaa
     4741 atcctctgct tctacatccg gtcttccttt tagctgattg accagtttcc atttatagtc
     4801 ttcatccgta aaaccttttt gctgaatgag
//
LOCUS       DAOPYB010000213         4819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_22452_length_4819_cov_7.273510, whole genome shotgun sequence.
ACCESSION   DAOPYB010000213 DAOPYB010000000
VERSION     DAOPYB010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22452_length_4819_cov_7.273510"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2702
                     /locus_tag="PKH58_11905"
     CDS             <1..2702
                     /locus_tag="PKH58_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNX89777.1"
                     /translation="FAQTQIKGVVKVADSETPLPGVTITLMQQNISTKTIGTGEFLLT
                     YLNPGVDDISIAKPGYLPQIKEIVLKENQTLDIGTVYLKMDVQEDMRQDAILQLNESD
                     FSEDEGRATQSVSSSISRGDVYTSQTSFSFSPMRFRIRGYEQEFESTYINGVAFNSLD
                     RGGFNYSSLGGLNDAMRNKDVFVGLDANSFSYGNLGSNTNIINRASAFAAGTKASVAY
                     SNRAYKLRGQVTYATGVLPSGWAFTVSGLFRWADEGIQQGTFYNSAGYFLAAEKIFNP
                     QHSLSLVTYGAPTMRGQASAGTQEVYDLTGTIYYNSYWGYQDGKKRNSRVVKSYDPTT
                     ILTHDFKISEKQRLRTGLAYHYSLYSSSALGFYNAKDPRPDYYRELPSFQTTDEAAAL
                     YTTRWQFIDGEPAVPSVSQINWDKLYRSNYDNNIVNPNGCANYMVERRHSNLMETVLN
                     STYTNQLTRALKLTAGVEAKYAKDMNYKTVDDLLGGNQWIDKDQFAERDFPSNPDIVQ
                     NDLNNPNRVVKKGDIFGYHYDINILHSSAYIQNEWNFSQLDVYYAGKVTYTQFNRYGY
                     MKNGRAPENSYGSGKTWWFVDPSVKAGATYKINGRNRLSANILAETLAPIPSGAYVSE
                     RIKDTRVPELDSRKVLSYDLNYSATFSAVRGRVSLFQTHILGATESTGYYDDNQRTFI
                     NFMLSDLNKRYRGIEAGVTVKLNRSFSISAAGTYADYRYTNNSLGTMSWENGAKADAT
                     DSVYTSGLKINSGPQLACNVTLDYFHPKMWFADITLNYFDNNYLDFAPDRFTKSSMAL
                     YENVPGAIEALGTQEKLKGGFLLDASIGKLFYLKNKKSLNFNLSLSNILNNTSMITGG
                     YQQGRMPIDTDTKLLDPTALNKFPNKYYYAWGFNLFFNIGYKF"
     gene            2732..3727
                     /locus_tag="PKH58_11910"
     CDS             2732..3727
                     /locus_tag="PKH58_11910"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HNX89778.1"
                     /translation="MKNKIIISALILATALFAGCTQTEFDAIPYNMGDSLKTTYTIVQ
                     LKSEFMKKTDAYSDADSLTNLYTSDLIMSGNEVVVDGYVTSVDTEGNVYKYFTFQENK
                     PNGQALKVSVDASGITSLYNIGQHVWIRCNNLYIGNYGQAPQLGSRYENTEKFRVDKT
                     TGKTFYRIEPGRMPLQLAETHVHYYGKPDPSIIKADTMTVAQINALKADTSIVRLVNK
                     LVCIKNAYFTGFDGSNKPLTGENLIFAPSTNGVGYPQLRMINDGTGNIAIASSEYAKF
                     ATKPLPTSNYRGNITVIVGWYKNYTDAAGVWQLTIRTLSDLGKGYEGYLSQINYK"
     gene            3745..>4819
                     /locus_tag="PKH58_11915"
     CDS             3745..>4819
                     /locus_tag="PKH58_11915"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HNX89779.1"
                     /translation="MEKKLLYGILMIISVFTYTSCSNAGDYVPNTPDEVQKVIYSELF
                     ADTLGGFTTVDVSGTQKWAIEKTRKYAYMSGFINPTNYANEDWLISPEIDLTDVTKAH
                     FSFDHVARYFTDPMNDATVWITDNYSESAPTTNWTQLHITPFTDPGSYTFGSSGQISL
                     TAYAGKKVRIAFKYVSTETKAGTWEVKNFSVSSGEAVVVNNVYGAGTEENPYTVLGAS
                     ENQSNKLAWVKGYIVGYVWYANKMNTFYFSSDTCTQKTNLLIADSLGGFYLSQCAAVQ
                     LPVGAVRNGINLSDNKSLIGKKVTLYGSLTPYFGITGVKNTSFYKLEDGTSGGTKPFD
                     PANAIFYETFATGLGLFTSQNVTG"
BASE COUNT         1625 a          828 c          955 g         1411 t
ORIGIN      
        1 tgtttgctca aacgcaaata aagggtgtgg tgaaggttgc tgactctgaa acaccgctgc
       61 cgggtgtgac aatcacactg atgcagcaaa atatttctac taaaacaatc gggacaggag
      121 aatttctcct tacttatctg aaccccggcg tggatgatat aagtatagca aaacccggtt
      181 atcttccaca gattaaagaa attgttctga aagaaaacca gacactcgat atcggaacag
      241 tatatctgaa aatggatgta caggaagata tgcgacagga tgcgatctta cagttaaacg
      301 aatctgactt cagcgaagat gaaggtcgcg ccactcagag tgtttcttcc agtatttcac
      361 ggggtgatgt atatacttcg caaacatcct tttcatttag cccgatgaga ttccgcatac
      421 gtggttacga acaggaattt gaatctacat atattaatgg tgtagccttc aattcattgg
      481 atagaggtgg attcaattat tctagtttgg gagggttgaa tgatgccatg cgaaacaagg
      541 atgtattcgt aggattggat gccaactcat tcagttatgg aaatctgggt tccaatacca
      601 acatcatcaa cagagcaagt gcatttgcag ccggaacaaa agcaagtgta gcatacagta
      661 accgcgctta caaactaaga ggtcaggtaa catatgccac cggcgtactg ccaagtggtt
      721 gggcatttac tgtgtccggt ttatttcgct gggccgatga aggtattcag caaggaacgt
      781 tttataactc agctggctat ttcttagctg ctgaaaagat attcaatcca cagcacagcc
      841 tgtcgttagt tacttacgga gctccaacca tgcgtggaca agcaagtgca ggtacgcagg
      901 aagtttatga tttaacagga acaatatatt ataattcata ctggggctac caggatggga
      961 aaaaacgaaa ctcccgcgta gtgaaaagtt atgaccctac aacaattctt acgcatgatt
     1021 tcaaaattag tgaaaaacaa cgtttacgca cagggcttgc atatcattat agcttgtaca
     1081 gcagttctgc acttggtttt tataatgcta aagatcctcg tccggattat tacagagaat
     1141 tgccaagttt tcaaacaaca gatgaagcag ctgcacttta taccacacgc tggcaattta
     1201 tagatggtga acctgcagtt ccttctgttt cgcaaatcaa ctgggataaa ctttatcgtt
     1261 caaattacga caataatata gtaaatccaa atggatgtgc taattatatg gtagaacgtc
     1321 gtcatagcaa tttgatggaa acggtattaa attctacata taccaatcaa ttaacaagag
     1381 cacttaagct gaccgctgga gttgaagcaa aatatgctaa agatatgaac tacaaaacgg
     1441 tggacgattt gttgggtgga aatcaatgga ttgacaaaga tcaatttgca gaacgcgatt
     1501 ttccatcaaa tccagatatt gttcaaaacg atttgaataa tccaaacaga gtggtgaaaa
     1561 aaggcgacat atttggttat cattacgata taaatatttt acatagcagc gcttacatcc
     1621 aaaatgaatg gaatttttct cagttggatg tgtattatgc cggaaaagtg acatacactc
     1681 aatttaaccg ttatggatat atgaaaaacg gacgtgctcc cgaaaattct tatggctcgg
     1741 gaaaaacatg gtggtttgtc gatccttcag ttaaagcagg tgctacttat aaaattaacg
     1801 gacgcaatag attatctgcc aatattcttg ctgaaacgtt agcaccaata ccaagtggtg
     1861 cttacgtttc ggaacgtata aaagatacac gcgttccaga gcttgacagc cgaaaagtgc
     1921 tttcgtatga tctgaattat tctgctacat tctctgctgt tagaggtaga gtgagtttgt
     1981 ttcaaacaca cattctcgga gcaaccgaaa gtacaggata ttatgatgat aatcaacgaa
     2041 cttttatcaa ttttatgctt tctgatttaa ataagcgcta tcgtggcata gaagccggag
     2101 tgacagtaaa actcaacaga agtttttcta tcagtgcggc aggtacttac gcagattaca
     2161 gatatactaa taattctttg ggaacaatga gttgggaaaa tggtgcaaaa gccgatgcta
     2221 cagattcagt atatacaagt ggtttgaaaa taaactcagg acctcaatta gcgtgcaatg
     2281 ttacgttgga ttattttcac ccgaaaatgt ggtttgcaga tattaccctg aattattttg
     2341 ataataatta tttggatttt gctcctgata gatttacaaa atcaagtatg gctttgtatg
     2401 aaaatgttcc cggagcgata gaagcacttg gtacgcaaga aaaactcaaa ggaggatttt
     2461 tgttagatgc atcgataggt aaattattct atttgaaaaa caaaaaatca ttgaatttca
     2521 atttaagttt aagtaatatc cttaacaata caagtatgat tactggtgga tatcaacaag
     2581 gacgtatgcc tattgataca gatacaaaac tattggatcc aactgcatta aataaatttc
     2641 cgaataaata ctattatgct tggggattca acttgttctt caacattgga tacaaattct
     2701 aaattacgaa aaaaaaaaaa taaagagaac aatgaaaaat aaaattatca taagcgcact
     2761 tattcttgca actgctttgt tcgctggttg cacgcaaacc gaatttgatg ctattccata
     2821 caatatggga gattctttga aaacgactta tacgattgta caactcaaat cggagtttat
     2881 gaaaaaaaca gatgcttatt cagatgcaga ttcacttacg aatctatata cctctgattt
     2941 aattatgtct ggtaatgaag tagttgttga tggatatgtt acttcggttg atacggaagg
     3001 aaatgtgtac aagtatttta ctttccaaga aaataagcca aacggacaag cgcttaaagt
     3061 atcagttgat gcttccggga taacttcgtt gtataatata ggacagcacg tttggatacg
     3121 ttgcaataat ttatacattg gtaactacgg acaagctcct cagttgggta gcagatatga
     3181 aaataccgag aaatttagag tagataaaac caccggaaaa acattctata ggattgaacc
     3241 gggacgcatg cctttacaac ttgcagaaac tcacgttcat tattacggaa aaccagatcc
     3301 gtccataatc aaagcggata ccatgactgt agctcaaatc aatgccttaa aagcagatac
     3361 atctatagta agacttgtaa ataaattggt ttgtatcaaa aatgcttatt ttactggttt
     3421 tgacggaagc aataaaccgc ttaccggtga aaatctgata ttcgcacctt ctacaaacgg
     3481 cgtaggttat cctcaacttc gtatgataaa tgatgggaca ggaaacattg ctattgcaag
     3541 tagcgaatat gcaaaatttg cgacaaaacc acttcctaca tcaaattaca gaggcaatat
     3601 aactgttata gtagggtggt ataaaaatta taccgatgcc gctggtgtat ggcaattaac
     3661 tatacgcaca ttaagtgact taggaaaagg atacgaaggg tatttaagtc aaataaatta
     3721 taaataactt tatagtcaag taaaatggaa aagaaattat tatatggaat tttaatgata
     3781 atatcagtat ttacatatac ttcgtgttca aatgctggtg attacgttcc aaatacacct
     3841 gatgaagtac agaaagtgat ttattctgaa ttatttgccg acacgttggg tggttttact
     3901 acagttgatg tatcaggaac tcaaaaatgg gcaattgaaa aaacaagaaa atacgcttat
     3961 atgtcaggat ttattaatcc taccaattat gcaaatgaag attggttgat ttcacctgaa
     4021 attgatttaa ctgacgttac caaagctcat ttctcatttg atcacgtagc tagatatttt
     4081 accgatccaa tgaatgatgc aaccgtttgg ataactgata attacagtga aagtgctcca
     4141 actacaaatt ggacacagtt gcacattact ccatttacag atccgggttc ttatactttt
     4201 ggaagttccg gacagattag tttaacagct tatgcaggta aaaaagtacg tattgccttc
     4261 aaatatgttt ctacagagac aaaggcaggt acttgggaag taaaaaactt ttcagtttct
     4321 tctggcgaag cagtggttgt aaataatgtt tatggggctg gtaccgaaga aaatccttat
     4381 actgtgttag gtgcaagcga aaaccaatca aacaaacttg cttgggtaaa aggttacatt
     4441 gtagggtatg tgtggtatgc aaacaaaatg aacacattct atttttcttc tgatacttgt
     4501 actcaaaaaa ccaacttgct tattgccgat tcgttaggag gattctattt atctcaatgt
     4561 gctgctgttc aattacctgt tggcgctgta cgaaatggaa tcaatttgag cgataataag
     4621 tctcttattg ggaaaaaagt tactctttat ggtagcctga caccatattt tggaataaca
     4681 ggagtgaaaa atacctcatt ctacaaatta gaagatggaa cttcaggtgg aacaaaacca
     4741 tttgatcctg ccaatgcaat tttctacgaa acatttgcaa ccggattagg attgtttact
     4801 tcacaaaacg ttacaggag
//
LOCUS       DAOPYB010000214         4816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_22473_length_4816_cov_11.874186, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000214 DAOPYB010000000
VERSION     DAOPYB010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22473_length_4816_cov_11.874186"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..1776)
                     /locus_tag="PKH58_11920"
     CDS             complement(241..1776)
                     /locus_tag="PKH58_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNX89780.1"
                     /translation="MYNEEDVFYKPRKYNTIELWKDVTDEQWYDANWQLKNSIRSVGQ
                     LKKVIKLNAFQESEIERTLVTLKNEGKEPLRITPYYASLMEEDPFHPVMLHGEKSGNR
                     LDPVFWQSVPTPANLLFPNTGLEGAMNESSRSYGAAYQRYPNRVALFVAENTSCASYC
                     VHCQRAKSLDSSVDVNINEINKGLFYIGYNKNINEVLVTGGDALRISRSRLQYVLEEL
                     SRIPHLRAIRIATRVPVVMPMGITDDLLELINISANKYNKGVDKYVYFMTHINHYHEI
                     TEDLEIAVKKIRKHGFTIRNQTVLLNHVNDYYKTLAETFRRMFWIGVHPYYLLQCHKE
                     KGIVHFITPIQIGKIYMKHLQGWISGVTVPRYAANIEGGGGKVLLMPSGHDTLNIKSN
                     IDDRISESFATINTWDGKKMFRYEALGRTSQEEFENGVRIMDTFIGRKGAFLPKLIIV
                     DNQGKHLETTNRTKLPALEKIIKSELLGYELFDKNMPLTNPADIADELEAQFEKSKFG
                     RKE"
     gene            complement(1791..2990)
                     /locus_tag="PKH58_11925"
     CDS             complement(1791..2990)
                     /locus_tag="PKH58_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX89781.1"
                     /translation="MTTTLIIIICIIFLIAYFFDLTSSRTRIPAVILLLLLGWGIKQL
                     TVFFHLEIPNLTSILPILGTIGLILIVLEGSLELELNKQKLPVIGKSLLLALLPMFIL
                     SLILAFLFMHFGQYSFRNSLINAIPLCIISSAIAIPSVKGLSMSNREFVIYESSLSDI
                     FGIIIFNFIAINGTINTQSFTHFALQILTIIIVSFVATIGLSFLLSRSKHHIKYVPII
                     LFVILIYSISKFYHLPGLVFVLLFGLFIGNIDELKQLKWLKVFDTDNLNSEVQKFKNI
                     VIEGTFLIRTLFFILFGFLIETEEILNTNTLVWSVGIVAGILIVRYIFLKFTKLPITP
                     LLYVAPRGLITILLFLSILPSQSISFVNKSLIIQVIVLTALVMMTGILKKPQKTTILS
                     ENEILEL"
     gene            complement(3540..3818)
                     /locus_tag="PKH58_11930"
     CDS             complement(3540..3818)
                     /locus_tag="PKH58_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89782.1"
                     /translation="MTTNKITVRAIVSADKQKTRKASPREQLKPFLPREFFSYFETYR
                     TLSLNKEIPFPFPFPTNIENPLTLFKWRGIMGEVGESAHPDTSGGDRG"
     gene            complement(3823..4251)
                     /locus_tag="PKH58_11935"
     CDS             complement(3823..4251)
                     /locus_tag="PKH58_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNX89783.1"
                     /translation="MTNKVVYPCLWFDGQARAAAEFYCSVFKNSEITADNQLVVTFEL
                     NGQKFMGLNGGPTFKFNEAVSFVVDCEAQEEIDYYWEKLTADGGQESNCGWLKDKFGV
                     SWQIVPSVLSKLMTDPEKAPRVTAAFMKMKKMDIETLLNA"
     gene            complement(4248..4658)
                     /locus_tag="PKH58_11940"
     CDS             complement(4248..4658)
                     /locus_tag="PKH58_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89784.1"
                     /translation="MKVKSIYLNLPVRDLEKTRNFWTNIGFTFNEQFSDDKALCLVLN
                     DGLIYSMLITHEYFSTFTNRTIADGSTTQVLLSIEVDSKEQVDNLVRLALENGGTRYR
                     KGEDNGWMYYDSFADLDGHQWEIIFMDISQMPKQ"
BASE COUNT         1463 a          923 c          843 g         1587 t
ORIGIN      
        1 cttaaatatc aatagaaagc gtgtttttgt attggttcat ttcccatcgg gaattgatat
       61 atttatcccc taatggggaa tttaatttat ttaataacct ttttctatgg atattaattt
      121 cctacgggaa aacagacaat tgtacctgaa ttggttaaat gtgtatttta catcgaactc
      181 acgttaacta ataaaaatcc tctaccagaa acattatgtt aagattggtg gaatgagttt
      241 tcattctttt ctaccgaatt ttgatttttc aaattgtgcc tccaattcat cggcaatatc
      301 agccggattg gtcaaaggca tatttttatc aaataattca taacccaaaa gttctgattt
      361 tataattttt tcgagtgcag gcaatttcgt tctgttggta gtttcaaggt gtttcccctg
      421 attgtcaact attatgagtt tgggaagaaa cgcgccttta cggccaataa atgtatccat
      481 aattctaaca ccattttcaa attcttcttg cgacgttctg cccagagcct catacctgaa
      541 catttttttc ccatcccagg tatttatcgt tgcaaaactt tcagaaattc tatcatcaat
      601 attgcttttt atattcaaag tatcatgtcc cgaaggcata agcaatactt ttccgccacc
      661 accttcaatg ttcgcagcat atctcggaac tgtaacaccc gaaatccacc cttgtaaatg
      721 tttcatatat attttcccaa tttggatagg ggtaataaaa tgaacaatcc ctttttcttt
      781 atgacactgc aataaataat aggggtgaac accaatccag aacatccgtc tgaacgtttc
      841 agccaaagtt ttataatagt cattcacatg atttagcaag acggtttgat ttctgatagt
      901 aaaaccgtgt ttgcgaattt ttttcaccgc gatttccaaa tcttccgtta tttcatgata
      961 gtgattgata tgtgtcataa aatatacata cttgtcaacc cctttgttgt atttgtttgc
     1021 cgaaatatta atcagttcca gcaaatcatc tgtaattccc attggcatta ctaccggcac
     1081 cctggttgca attctaatgg cacgcaggtg aggtatccgg cttaattctt ctaatacata
     1141 ttgcagtcgg gatctgctga tcctgagcgc gtcgcccccc gttacaagca cttcatttat
     1201 gtttttattg tatccgatat aaaacaatcc tttatttatt tcattgatat taacatctac
     1261 tgagctgtct aatgattttg cccgctggca gtgaacacaa taggaggcac aactggtgtt
     1321 ttcagccaca aaaagggcaa cccggtttgg ataccgttga taagctgccc cataactccg
     1381 tgaactctcg ttcatagcgc cttcaagtcc ggtattggga aataataagt ttgcaggtgt
     1441 aggcacactt tgccaaaaaa caggatcaag tctgtttccg gatttttcgc cgtgtaacat
     1501 taccgggtga aatggatctt cctccataag tgatgcgtaa taaggcgtaa tgcgcaatgg
     1561 ttctttccct tcatttttta atgttactaa cgtcctttct atttcacttt cctgaaacgc
     1621 atttaacttt attacctttt tcaactgtcc gacacttcgt atcgaatttt tcaattgcca
     1681 gttggcatca taccattgtt catctgttac gtctttccat aattcaatgg tgttgtactt
     1741 tctcggtttg tagaaaacat cttcttcgtt atacataagc tttatttagt ttataattcc
     1801 agtatttcat tttctgataa aatggttgtc ttttgtggtt ttttcagaat tcctgtcatc
     1861 atgaccaaag ctgtaagaac aattacctga atgataagcg atttattgac aaaagatata
     1921 ctttgtgagg gtaaaatgga cagaaacagc agaatagtta tcaaccctct tggagcaaca
     1981 taaagcagag gagtaatagg aagttttgta aatttcaaaa agatataacg cactattaat
     2041 atacctgcaa caattcctac cgaccaaaca agtgtatttg tatttaaaat ctcttcggtt
     2101 tcgataagaa aaccgaacag tatgaaaaac aatgttctga tcagaaaagt accttcaatg
     2161 acgatgttct tgaatttttg aacttctgaa tttaaattat cagtatcaaa aacctttaac
     2221 catttgagtt gttttaactc atcaatattg ccgataaaaa gtccgaaaag taatacaaaa
     2281 accaagcctg gcaaatgata aaattttgaa attgaataaa ttaaaattac aaataaaatt
     2341 atcgggacat atttaatatg atgtttgctt ctgcttaaca agaaagatag tcctatggtg
     2401 gctacaaaag atacgattat aatagtcaaa atttgtaagg cgaaatgagt aaaggattgt
     2461 gtattgattg ttccattgat ggcgataaaa ttgaaaatta ttatgccaaa aatgtcagat
     2521 aagctacttt cgtaaatgac aaattcacga ttcgacattg ataagccttt tacacttgga
     2581 atggcaatag cactgctgat aatacataat gggattgcat ttatcaaact gttcctgaat
     2641 gaatattgtc cgaaatgcat aaacaaaaaa gccagaatta agctaagaat aaacattggt
     2701 aacaaggcaa gtaagagcga tttgccaatt acaggcaact tttgtttgtt taattccaat
     2761 tcaagggagc cttcgagcac aataagtatt agtccgatag ttcctaaaat gggtaagata
     2821 gatgtaagat ttggaatttc aagatgaaaa aaaacggtga gttgttttat tccccagccc
     2881 aacaataaaa gaagtatcac ggcaggaatt cttgttcttg aagatgtcag gtcaaagaaa
     2941 taggctatca gaaatattat gcaaatgatt attattaaag ttgttgtcat actgtatttc
     3001 ttttactatt tgttggcaat ggctcgccaa ttggcgaagg ggagattttt gcaaaaacca
     3061 tgcaacgaag aacaaatatc aaaatgtaaa taaggtcaat agaaacacag catcattttc
     3121 tgagctaaac tgcttccgga tgatgtcaat tatgcttcat tgttcctgct ttttttattg
     3181 cttacacttt gttttgtaat aataacgatt tataaattaa taacaacaat gaattatcat
     3241 tctttaagaa aattattgaa tgattttata aatctgtcaa cggcttcgat ttatggggga
     3301 aacattatac attgtggtaa tataatgaat tggcacagat tacaaaaacg ctgaatctga
     3361 gaagtatttg cgggattgtg cttgtttgac ttccaatttc cctcgtgaaa cgcgacaaaa
     3421 caaaaacacg ttatctattg atctttaagt cttaaaggac aatttctaaa gcagcactga
     3481 tacttcaagc aataaggata ccatttgtcc cgcatatggt aaacttcctg ctgctcgccc
     3541 taacccctat cgcctcccga agtgtcggga tgggcacttt ccccgacctc tcccataatc
     3601 cctctccatt tgaaaagggt cagtggattt tctatattgg ttgggaaagg aaaaggaaaa
     3661 ggaatttctt tatttaatga gagggtgcga taagtctcaa aatagctgaa aaactcgcgg
     3721 ggaaggaaag gctttaattg ttcgcgggga gaggctttcc gggttttttg tttgtctgcc
     3781 gacacaattg cccgaacagt gattttatta gttgtcattc aattaagcat ttaacagtgt
     3841 ttcgatatcc atttttttca ttttcataaa tgcagccgtt acccgtggcg ctttctccgg
     3901 atctgtcata agttttgaca gtacggaagg aacgatttgc cacgaaactc caaacttatc
     3961 tttgagccag ccgcaattac tttcctgtcc accgtccgcg gtcagctttt cccaataata
     4021 atcaatttct tcctgggctt cacagtccac aacgaaggat acggcttcgt taaacttaaa
     4081 ggttgggccg ccattgagac ccataaattt ctgtccgttg agttcaaagg tcacaaccaa
     4141 ttgattgtcc gctgtaattt cagagttttt gaaaacagaa caataaaact ctgccgctgc
     4201 tctggcttgt ccgtcaaacc acaagcaagg ataaacaacc ttgtttgtca ttgttttggc
     4261 atttgactga tgtccataaa tataatttcc cattgatgcc cgtcaaggtc ggcaaaactg
     4321 tcgtaataca tccaaccgtt gtcttcacct ttcctgtaac gtgttcctcc attttccaga
     4381 gcaagtctta caaggttgtc aacctgttct ttgctgtcaa cttctatgga taacaaaacc
     4441 tgggttgttg aaccgtcggc aattgtgcgg tttgtgaatg tgctgaaata ttcgtgtgta
     4501 atcagcatag aataaataag tccgtcattc aggaccaggc acagtgcttt gtcgtccgaa
     4561 aactgctcat tgaacgtaaa gccaatattt gtccagaaat ttcttgtttt ttcaaggtcc
     4621 ctcaccggta agtttaggta tattgatttt actttcatag gtaaataatt tattttttaa
     4681 ttgtcaaatg gaattctgtt cttcagctca ccactttaat attaaatacg cccgcttttt
     4741 tagcaccgta aatcctcagc cacaaaggca aatacatttc tgttcccctt gccgctgtta
     4801 aatcgcccag gtcaat
//
LOCUS       DAOPYB010000215         4795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_22653_length_4795_cov_10.455485, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000215 DAOPYB010000000
VERSION     DAOPYB010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22653_length_4795_cov_10.455485"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..>2053)
                     /locus_tag="PKH58_11945"
     CDS             complement(44..>2053)
                     /locus_tag="PKH58_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX89785.1"
                     /translation="DFHFILHYAGYVKVYFNNIPVVTERWRTAWNPNDFKFKYNLRAG
                     EKIPVRIEWEPDGAVSYISLKALSPLAHQEQNKLSLWSEMADEIDYYFVYGSTMDDII
                     SGYRKLTGKSQIMPEWAMGFWQSRERYKTQHELLTTLSEFRKRNIPIDNIVQDWNYWP
                     ETEWGSHKFDPQRFPDPVGLVDSVHKMNGHIMISVWPKFYITTDNYKAFDKNGWMYQQ
                     AVKDSIRDWVGPGYVGSFYDAYTEGARKLFWKQMKGSLYDKGFDAWWMDASEPNVQDN
                     TDIDYRKKLCGPTALGPSTKYFNAYALMNAEAIYDGQRSVDPDKRVFLLTRSGFAGLQ
                     RYSTATWSGDIGTRWEDMKAQISAGLNFALSGIPYWTMDIGGFCTEKRYENAQRLFDQ
                     TGTEDNDLKEWRELNTRWYQFGAFAPLYRAHGQFPLREIFNIAPENHPAYQSILYYSK
                     LRYRLMPYIYSLAGMTHFNDYTIMRALVMDYGKDTVVNNISDQYMFGNALMVCPVYEY
                     KAVKREVYFPNTTGWYDFYTGKHVQGGTHMQTDAPYSHMPVFVPEGAIIPFGPDIQFT
                     REKKPESITLYVYGGKNGSFTLYEDEGTNYNYEKGKYSMITFTYDEKNGLLEIGNRIG
                     SFTGMLEKREFNVVFVDKVTPESCNPDTVGMKVKYSGQKTTLKLR"
     assembly_gap    2054..2153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2154..2369)
                     /locus_tag="PKH58_11950"
     CDS             complement(<2154..2369)
                     /locus_tag="PKH58_11950"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNX89786.1"
                     /translation="MKRFKLFLLLLIATLTGCNHAFCQKIADGIIFPVLSDSHTGVKK
                     IRIQVVSDKIIRVTATAGNKFSGVKSLI"
     gene            complement(3064..4638)
                     /locus_tag="PKH58_11955"
     CDS             complement(3064..4638)
                     /locus_tag="PKH58_11955"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNX89787.1"
                     /translation="MKKNNLLILICCIYSLFVSCDPDNLLDNNDLDNSSNETELASDY
                     DWDAGNATTISLNNNNITSGSDAVTIAGSIATITKAGYYIVNGTLTNGQLIVAVDSGI
                     VKIQFDGVNIANTTGSPFYIKKSSKTILFLADGSSNMISDASSYVSSEEPNAAIFSNS
                     YLAITGNGSLTVKGNFNDAISTDDELIINNGTITATGKDDAIRGKNFLKVNGGTITAT
                     GGTGHALKSDNTDKAGYGYIKIDGGTLNLASSSADGIHAVKRVIINSGNLTISATGSQ
                     ALHSDSLVFINDGTTIIKSSKEGIESPYIVINGGELNINATDDGFNSTFGNGGEAIDK
                     SCLTINGGKTSVDVVKGDGLDSNGTGNMTGGTVVVHGPSSAPEVALDVNGDFKVTGGT
                     MIASGPNSGNMIEGPATSSSQYSVLIKFGASSGQFGGPGGGTGTNQIAAGTIINIRDS
                     SGNSLISFVPVRACYYVIFSSPLLKSGSTYTVYTGSTVTGETIWNGYYQNGTATGGTS
                     RGTFTVSGKLTTVNLN"
BASE COUNT         1389 a          978 c          886 g         1442 t
ORIGIN      
        1 tctatatata taactgtttc tgacatattc atatttcatc tctttatctg agtttcaagg
       61 tcgttttttg tccggaatac tttaccttca tcccaacagt gtctggatta caggattcag
      121 gtgttacttt gtcaacaaac accacgttga attcacgttt ttccaacatt cctgtgaatg
      181 aacctattct attgccaatt tccagaagtc cgtttttttc gtcataagtg aatgtaatca
      241 ttgaatactt gcccttttca tagttgtagt tggttccttc gtcttcataa agggtgaatg
      301 aaccattttt gcctccataa acatacaagg ttatgctttc aggctttttc tcacgggtaa
      361 attgaatatc gggaccgaac ggaataattg ctccttcggg aacaaatacc ggcatgtgcg
      421 aataaggtgc gtcggtttgc atatgtgtac ctccctgcac gtgtttaccg gtgtagaaat
      481 cataccaacc tgtggtattg gggaaataca cctctcgttt caccgctttg tattcataaa
      541 ccggacatac catcagggca ttaccgaaca tatattgatc gctgatgtta tttacaacag
      601 tatccttacc ataatccatt accagagcac gcatgatggt atagtcgttg aaatgggtca
      661 tacctgccag cgaatagatg tatggcatca gccggtaacg aagttttgaa taatacaata
      721 ttgactgata tgcagggtga ttctcaggcg cgatgttgaa tatctcccgc agcggaaatt
      781 gtccgtgagc acgataaagg ggagcaaagg cgccaaactg gtaccagcgt gtattgagtt
      841 cccgccattc tttcagatcg ttatcctcag ttccggtttg atcgaacaat cgctgggcgt
      901 tttcatatcg tttctctgta caaaaacctc cgatatccat tgtccagtat ggaatacccg
      961 acaaggcaaa attaagtccg gctgaaatct gggctttcat atcctcccac cgcgtaccaa
     1021 tatcgccact ccaggtagca gtggagtatc tttgcaaccc tgcaaatccg gaacgtgtaa
     1081 gcaggaatac ccttttatcg ggatcaacac tgcgctgacc gtcatagatt gcttcggcgt
     1141 tcatcagtgc atatgcgttg aaatatttgg tcgatggtcc gagcgcagtt gggccgcata
     1201 gctttttacg gtagtcaatg tccgtgttgt cctgcacatt gggttcagat gcatccatcc
     1261 accaggcatc aaaacctttg tcgtaaaggc tgcctttcat ctgtttccag aaaagtttgc
     1321 gggcaccttc ggtatatgca tcgtaaaaag agccgacgta accagggccc acccaatctc
     1381 gaatgctgtc ttttacagct tgctgataca tccagccgtt tttgtcaaat gctttataat
     1441 tatcggtagt aatgtaaaac tttggccata cagaaatcat gatgtgtccg ttcattttgt
     1501 gtacactatc gaccaatcca actggatccg ggaagcgctg cggatcaaac ttatgactgc
     1561 cccattcggt ttcgggccag tagttccaat cctgtacaat attgtcgatc gggatatttc
     1621 ttttacgaaa ttccgataaa gtggtcagca gttcgtgctg agttttatag cgctcccggc
     1681 tttgccagaa acccattgcc cattcgggca taatttgaga tttacctgta agcttccgat
     1741 aaccgctgat tatgtcatcc atcgtgctgc cataaacaaa ataataatca atttcatcgg
     1801 ccatctcact ccagagcgac aacttgtttt gttcctgatg agcgagtgga cttaatgctt
     1861 tgagggagat ataagagact gcaccatccg gttcccattc gattcgaacg ggtatttttt
     1921 cgccagcacg tagattgtat ttaaatttaa agtcattggg gttccaggct gtacgccagc
     1981 gttctgtcac aacaggaata ttattaaaat acactttgac atatccggca taatgcagta
     2041 taaaatgaaa atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaataaga
     2161 cttttcaccc cggaaaactt attccctgct gtagcggtaa ctctgataat tttatcggat
     2221 acaacctgta tccggatttt tttgacacct gtgtgggaat ctgacaatac cggaaaaatt
     2281 ataccatcgg caattttttg acaaaatgca tgattgcaac ccgttaaagt ggctatgagc
     2341 aacaataaga acagtttaaa tcttttcatg aaattattct attataataa attactttta
     2401 cttcgttgtc tgtgtttaat aaaaaaaatc taagcatgca aaaatacact tttaaactaa
     2461 ttaacgacca gattacgtca tctatttcgt ttatcactaa aataacaaat cagataagat
     2521 gtttttcatt attgaaatta ttacataaca aggcaaatta tgaataaaaa agtcagctat
     2581 ttttttaacc ggtttaccca tcagattgaa ttcaacgatc cgaaaaaaaa tcagaaaatt
     2641 tttcaagtaa aaatcaggta tgagttaaac tgaattcagg taccagatcg ggcagacaca
     2701 aaagaaaaat cagatgcaaa ttgaatacta aagacaaagt attcggagta atttccgcat
     2761 aatgaaattc cattagtaag aaattggaag gagtgcaagc tcgaaaagac tctatttgaa
     2821 aaaaataaaa aaggagtctc agcattgtga aactcctctt acaaaataaa aaagtagcgt
     2881 cgttgcgact atcaattcag tggttgtgta cttgcgtcca gaaagaaatt aaatttcttt
     2941 ttataaccgc ttttcaattt gttttcacag acctgatatt ccaaactgct tcggttgttg
     3001 gataatattt cacttttttt acacgatatt aagtctgaaa aaaatcagtt taaaaagaag
     3061 ttattaattc agattaacag tagttagttt acccgaaacg gtaaacgtac cgcgggaagt
     3121 tcctccggtt gctgtaccat tctgataata tccgttccag atggtttcac cggttacagt
     3181 tgaacctgta taaacggtgt atgtggaacc gctcttaagt agcggagaag agaaaattac
     3241 ataataacat gcacgaacag gaacaaaact tatgagactg ttccctgaac tatcccggat
     3301 attgataata gttcctgctg caatctgatt ggtgcctgtt ccaccacctg gacctccaaa
     3361 ctgacctgaa ctggccccaa atttaatcaa cactgaatat tgcgatgaac tggtagcagg
     3421 tccttctatc atattgcctg aatttggtcc cgaagctatc atggttcccc cggtaacttt
     3481 aaaatcgcca ttcacatcca gagctacttc aggtgcactt gacggaccgt gaacaaccac
     3541 tgtacctccg gtcatgttgc cagttccgtt actgtcgagt ccatcgcctt tgactacatc
     3601 aaccgaggtt ttaccaccat ttatcgtcag acagctttta tcaattgctt ctccgccatt
     3661 tccaaaagtc gagttaaatc catcgtctgt ggcattaata ttcagttcgc caccgtttat
     3721 tactatataa ggactttcaa ttccttcttt ggacgatttg atgatagttg ttccgtcatt
     3781 aataaacacc agcgaatcgg aatgcaacgc ctgacttccc gttgctgata tcgtcagatt
     3841 accggagtta ataattaccc gtttgaccgc atggatacca tcagccgacg aagatgctag
     3901 attaagtgta ccgccatcaa ttttaatata gccataaccg gctttatccg tgttatccga
     3961 tttaagcgca tgtccggttc caccagtagc cgttattgtt cctccgttaa cttttaaaaa
     4021 gttttttcca cgtattgcgt catccttacc tgttgctgtt atggttccgt tgtttataat
     4081 gagttcatca tcagtcgaaa ttgcatcatt aaaattacct ttaacagtga gtgaaccatt
     4141 gcctgtaatt gccaggtatg aattgctgaa tatcgcagca ttgggttctt cacttgacac
     4201 atacgaagaa gcgtcagaaa tcatatttga gcttccgtca gcaaggaaga gtatggtttt
     4261 ggatgatttt ttgatataaa acggagatcc ggtcgtgttg gctatgttta caccgtcgaa
     4321 ctgtatctta acaatgccag aatccacggc tacgataagc tgcccgtttg tcaatgttcc
     4381 attcacgata taatacccgg ctttggtaat tgtagcaatg gaacctgcaa tagtaactgc
     4441 atccgagcca gaggtgatat tattgttgtt caaagaaata gtagttgcat ttccagcatc
     4501 ccagtcataa tcggaagcta actctgtttc attggaagaa ttgtccaaat cattgttgtc
     4561 cagcaaattg tcagggtcac atgacacaaa aagtgagtaa atacaacaaa taaggatcaa
     4621 taaattattt tttttcattt tcaaaagaaa ttagggtgaa gaattaagcg aatagaatta
     4681 ttatttaagt ctgataataa ttaatcaatg tattgactgg tttttaatta aaaagtttaa
     4741 ccgaaggaat tttaaatatt ctgaataaaa acaaacttat caaaacataa atcaa
//
LOCUS       DAOPYB010000216         4788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_22703_length_4788_cov_8.197127, whole genome shotgun sequence.
ACCESSION   DAOPYB010000216 DAOPYB010000000
VERSION     DAOPYB010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22703_length_4788_cov_8.197127"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1578)
                     /locus_tag="PKH58_11960"
     CDS             complement(193..1578)
                     /locus_tag="PKH58_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNX89788.1"
                     /translation="MKHFNKFYILGICMVSAMGGLLFGYDWVVIGGAKPFYERFFDIV
                     NYPTLQAWAMSAALIGCVVGAVTSGVVSDRFGRKWPLFFSALLFTVASFGTGAANTYF
                     LFIVFRLIGGVGIGLASALSPMYIAEVAPAHLRGRFVSLNQLTIVVGILGAQIVNMMI
                     AREVPLGATDEFIRTSWNGQFAWRWMFYACAVPSILFLVLTVFLPESPRWLIKAHKDD
                     KAIPTLQKIGGEEYARDEQMNIRNSLNDITEKVDFKALFDPKLRNILLIGIVIAVFQQ
                     WCGINTVFNYAEEIFSAAGYGVSDTLFNIVITGSVNLVFTLVAMFTVDRWGRKKLMIL
                     GAVGLAITYVFLGAAFYFQLKGIAVLSLVVIAIAIYAMSLAPITWVILSEIFPNRIRG
                     AAMALATFALWAACFILTYTFPLLNASLGAYGTFWVYSVICLVSFVFIMLKLPETKGK
                     SLEDIEKELVK"
     gene            1789..2679
                     /locus_tag="PKH58_11965"
     CDS             1789..2679
                     /locus_tag="PKH58_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX89789.1"
                     /translation="MAKIKSGFPGERAIVLPQSVVQEFRKDDLGKLLYITDIGFYPKA
                     VFHFRKRSETEALQYIIMYCIYGEGWIEVDGNEQKLTNGEVVILPKGKAHSYGSDPQK
                     PWSIYWIHFDGDMASYFCEGLEKPLLIKIEKDSRIEDRLELFEDIFSILKNGYSKMNL
                     DYSVTVLFHFLGSLKFINAFRCSQSDRQTKRDMIEEAIHFMRENLQRQIKLEEIAQYT
                     KYSVSHFSTLFKEKTGYSPLNYFNQLRIQAACHHLDFTDLHINQIAMMTGFKDQFYFS
                     RIFRSIMGSSPSDYRKRIKG"
     gene            2907..3590
                     /locus_tag="PKH58_11970"
     CDS             2907..3590
                     /locus_tag="PKH58_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HNX89790.1"
                     /translation="MLELLKETVYRANLDLVKHGLVIFTWGNASAIDRTLNLVVIKPS
                     GVSYEEMKSTDMVVVDLDGNIIEGKLKPSSDTATHLALYKAFPNIGGVVHTHSTYATS
                     WAQAGCEIPNIGTTHADYFSDTIPCTRDMKTQEVEGEYEKETGNVIIERFKNINPDYV
                     PGVLVKNHGPFSWGKDANDAVHNAVVMEQVAKMAYISFGINPNLTMNELLIKKHFYRK
                     HGPNAYYGQ"
     gene            3669..>4788
                     /locus_tag="PKH58_11975"
     CDS             3669..>4788
                     /locus_tag="PKH58_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HNX89791.1"
                     /translation="MINYNDFEVWFVTGAQLLYGGDAVVKVDAHSNEMVAGLNASGNL
                     PVKVVYKGTANSSSEIAEIMRAANGDTKCIGLITWMHTFSPAKMWIHGLMDFKKPLLH
                     LHTQFNEQIPWSEIDMDYMNLNQSAHGDREFGFITSRLRKPRKVVVGFWKDKSTHDKI
                     SGWMRVCAAWADAQGMQIIRFGDNMNNVAVTDGDKVEAEIRLGYHVDNAPIAKLVPYV
                     NAVTEAEIDELISEYEKLYDFADDCKKGAEKHISVRQAAAQEIGLRRFLQDNNATAFT
                     TSFNELEGMSQLMGFASQRLMAEGYGFGAEGDWKTAALTRTMWVMAQGLPGGCSFLED
                     YVLNFDGANSSILQSHMLEINPEIAKAKPRIEVHFLGIG"
BASE COUNT         1581 a          921 c          925 g         1361 t
ORIGIN      
        1 gataaacccg tttctcgagg aatatgggat ttttttcagc tttaccggtt tcgtaagtag
       61 gtatcgcaac ttcttcactc catacgcgaa cgacactttc gttcactgta gattttatca
      121 gatattcagt aattttttcc acttaatcta aaatttaaat aaacgacaca ataatggcaa
      181 aataacacat ttctatttaa ccagttcctt ttctatatct tccaatgact taccttttgt
      241 ttcgggtagt ttcagcatga taaacacaaa acttaccaga caaattaccg aatacaccca
      301 gaacgttccg taagctccta atgatgcatt cagcaaaggg aaagtatagg taagaataaa
      361 acaggctgcc cagagggcaa acgtagccag tgccatggca gctccccgaa tacggtttgg
      421 gaagatttca gataaaatca cccatgtaat gggagcgagt gacatggcat aaatagcgat
      481 agcaatgaca actaacgata atacagcgat acctttgagc tggaaataaa acgcggcacc
      541 cagaaaaaca taagttatag ccaatccgac agcccccagt atcattaatt tttttcttcc
      601 ccatctatcg actgtgaaca tagccacaag agtaaatacc aaattaacac ttccggtgat
      661 aacaatatta aacaaggtat cactcactcc atatccggca gctgaaaaga tttcttcagc
      721 ataattaaat acggtattta taccgcacca ttgctgaaag accgcgatta ctatccctat
      781 taatagtata tttcggagct ttggatcgaa aagggcttta aaatcaactt tttcagtaat
      841 atcgtttaag gaattacgga tattcatctg ttcatcacgg gcatattcct ctccacctat
      901 tttttgaagt gtgggtatgg ctttatcatc cttgtgagct ttaattaacc agcgggggct
      961 ttccggaaga aaaaccgtta atactaaaaa cagaattgaa ggaactgcac aggcataaaa
     1021 catccatctc cacgcaaact gaccattcca tgaagttcgt atgaattcat cagtagctcc
     1081 gagaggcact tccctggcaa tcatcatatt gactatttgt gcaccaagaa ttccgacaac
     1141 aatcgtcaat tgattgagag aaacaaaccg gcctcttaaa tgtgcgggag caacctcagc
     1201 gatatacatg ggagaaagag ctgaagctaa tccaattcca acgcctccga tcagacgaaa
     1261 cacaataaac agaaaataag tattggctgc accggtaccg aacgacgcta ctgtaaataa
     1321 taatgcggaa aaaaacaggg gccatttccg gccaaaccga tcactcacca ctccggaagt
     1381 aaccgctccg acaacgcaac caatcagagc agcgctcata gcccaggctt gtaatgtcgg
     1441 ataatttaca atatcaaaaa aacgctcgta aaaaggtttt gcgccgccaa taactaccca
     1501 gtcgtaacca aaaagcaatc ctcccatggc agataccatg cagatgccca gaatgtagaa
     1561 tttgttgaaa tgcttcattt tttcgataaa ttggaattat gtgccatgaa acaggatata
     1621 gatttgttct tccggtgtga ggctttaatt ctgttgatat ttaattttgt tgaattgaat
     1681 aagaataaag atacaaagta atgataatta ttttcctttt aaaatagata aaataattat
     1741 ttgcatggat tattttacta tttttgtttg aaatccaatt ataacagaat ggcaaagatt
     1801 aaaagtggat tccccggtga acgggcaata gtactacccc agtcagttgt acaagaattc
     1861 cggaaggatg atttaggaaa attattatat ataaccgata ttggttttta tccaaaagca
     1921 gtatttcact tcagaaagag aagtgaaacc gaagctcttc aatatattat catgtattgt
     1981 atttatggtg aaggatggat tgaggtcgat ggcaatgaac aaaagctcac gaatggtgaa
     2041 gttgttattt taccaaaggg gaaagcacac agttatggaa gtgatccgca aaaaccatgg
     2101 agtatttact ggattcactt tgatggcgat atggcatctt atttttgtga gggattggaa
     2161 aaaccattat taataaagat tgaaaaagat tccagaatag aagacaggct ggagcttttt
     2221 gaagacatct tctccatact taaaaatggt tacagtaaaa tgaatcttga ctatagcgtt
     2281 actgtacttt ttcattttct gggatctctg aaattcatta atgcttttcg ttgcagtcaa
     2341 tcagatcgtc agacaaaaag agacatgatt gaagaagcca ttcattttat gagagagaat
     2401 ttacagcgtc agataaaact tgaagagatt gctcaatata cgaaatattc cgtttcacat
     2461 ttcagcacac ttttcaagga aaagacgggt tacagtccac taaattattt caatcagcta
     2521 cgcatacaag cagcctgtca tcatctggat tttactgatc tgcacataaa tcaaatagca
     2581 atgatgactg gtttcaaaga tcaattttat ttcagccgga tatttagatc aattatggga
     2641 agttcaccaa gtgattaccg aaagcggatt aaaggttaat ttttttgata attacgtcgc
     2701 tgtgagttac aaacctactc tatttcactt cctctatatt acataatttg ctatagctac
     2761 atttaacttt aagaacattg aattcaccca atattatgtt gcaaaacaca ttaattacaa
     2821 cgcatatttt cgaaaagtat taatacattt gtgaactttt ttaagtgtac cgcatacact
     2881 taattttatc gttaacacta tatcaaatgc ttgaattatt aaaagaaacc gtttaccgtg
     2941 caaatttaga tttagtgaag catggtttgg taattttcac atggggaaat gccagcgcca
     3001 ttgacagaac actcaatctt gtagtcataa agccatcagg agtatcgtac gaggaaatga
     3061 aatccaccga tatggtcgtt gttgatctcg atggaaatat tattgaagga aaacttaaac
     3121 cttcatctga cactgccacc catctggctc tatacaaggc ttttccgaat atcggaggag
     3181 tagttcacac gcattccact tatgcaacat cgtgggctca ggcaggatgt gaaataccaa
     3241 acataggtac cacgcatgcc gattatttca gcgatactat tccctgtacg cgagatatga
     3301 aaacacagga agtggaaggc gagtatgaaa aggaaaccgg aaatgttatt attgaacgat
     3361 tcaaaaatat caatccggat tacgttccgg gagttttagt gaaaaaccat gggcctttca
     3421 gttggggaaa agatgccaac gatgctgttc acaacgccgt tgtaatggaa caagttgcca
     3481 aaatggccta catctcattt ggaatcaatc cgaatttaac tatgaacgaa cttctgataa
     3541 agaagcattt ctacagaaaa cacgggccaa acgcctatta cggacaataa acataaaata
     3601 aaaaaataat taaccctcct taattatccg ctgaaaaagg cattaaggat taaaatttaa
     3661 aaatcaatat gataaattac aatgattttg aagtatggtt tgtaacggga gcacaactcc
     3721 tgtacggtgg cgacgctgtt gtaaaagtgg acgcacactc aaacgaaatg gttgcaggat
     3781 taaacgcctc aggcaatctt cctgtaaaag tagtatataa aggaacagct aattcttcct
     3841 cagaaatagc agaaattatg cgtgctgcaa acggtgatac aaaatgtatc ggtttgatca
     3901 catggatgca tactttctct cctgcaaaaa tgtggattca cggtttgatg gacttcaaaa
     3961 aaccgttatt gcaccttcat actcaattca acgagcaaat tccttggtcg gaaattgata
     4021 tggactatat gaacctgaac caatcggctc acggtgaccg cgaattcggt ttcattacca
     4081 gccgtttgcg caaaccacgc aaagtggtgg ttggtttctg gaaagataaa tcaactcacg
     4141 ataaaatctc tggatggatg cgcgtttgtg ctgcatgggc tgatgctcag ggcatgcaaa
     4201 ttatccgttt cggtgataac atgaacaatg tggctgtaac cgatggcgac aaagtagaag
     4261 ctgaaatccg tttgggctac cacgtggata atgctcctat cgctaaattg gtaccttatg
     4321 taaatgcagt taccgaggct gaaatcgatg aattgatctc tgaatacgaa aaactatacg
     4381 actttgctga tgattgtaag aaaggtgccg aaaaacacat ttctgtacgt caggcagcag
     4441 ctcaggaaat tggtcttcgt cgtttcttgc aagataataa cgcaactgct ttcactacca
     4501 gctttaacga attggaagga atgtctcaat tgatgggatt tgcttctcag cgtttgatgg
     4561 ctgaaggtta tggttttggt gcagaaggtg actggaaaac agctgctctt acccgtacta
     4621 tgtgggttat ggctcagggc ttaccaggtg gttgttcatt cctcgaagat tatgtgttaa
     4681 actttgatgg tgctaatagc tctattcttc aatcacacat gttggaaatc aatcctgaaa
     4741 ttgcaaaagc aaaaccacgt atcgaagttc atttcttagg aattggtg
//
LOCUS       DAOPYB010000217         4786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_22715_length_4786_cov_11.500951, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000217 DAOPYB010000000
VERSION     DAOPYB010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22715_length_4786_cov_11.500951"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3503)
                     /locus_tag="PKH58_11980"
     CDS             complement(<1..>3503)
                     /locus_tag="PKH58_11980"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89792.1"
                     /translation="GATADVALTANADMTCDFVWYRNDLIPNVGTTASALTAPLSNTG
                     VAASLNPYYYTVVVTNANGCVTTSAPYEVSVVENPTVTINASETTVCAGGEITLTSYV
                     GNILPENVNYSWMLGVVNVGTQPNLTLSNLTVGDYTYTLNVETVGGCIGTANITIHVV
                     ADPTLTVTPNTPTTLCSGSTVDLGASITAGGVAGGEVYTWQRNGVTVYGATGNSFTDE
                     LTNTNINNIQYLYTATVEQAASGCAATANSAAVTVLAAPAVSISGNSVVCSGTNVSLT
                     ANVPGGATYIWFLDGVNVGADAAYSASHSQRTAPYVFTLQVTSGGCTMVSEPYNVYVN
                     SNPQLVVTADDINYCAGGQVTLTANVGAVDSEDITYNWSPAGGTAIGNTLLTPALSDA
                     TYTYSVTATSNGCQATGSITLTRADAPTILATTLADADIYFLCDGGNVKLTCVPATDN
                     YIWQHNGVVLEGVTGSQLIESLSQGTHNFTISNHPGCLASVDIPVSVQPTVFLGIGGS
                     TEICEGGTVTFEVQFTDPNYYFENTYNNNNNYNNWYEDNRLMVLDPDNTWQIDVHPTA
                     ANQGHTYQYLLVDEDRGCYATSEVITIDVHPRPTVTLTPEMSPVCAGAEITLFTNISG
                     YQSENPVYKLNGVITTLNPTVTINDDTTFTFELVASTAPSCDVNASVDITVTDPIETP
                     AVTINSLDATTWTALPEELVCNHGQVLVSADFAGGARPDYQYAWYKNGILMEGNTASS
                     FIDELSNLGDEPTTCAYTVVVSSTIAGCENITATSVNVRILPDLNMIISGPTDLCAGS
                     NAQYIATCNDFPEAAMFLGGADYTWYVDDIEFLDETASFTAPASEIAPQADPYTYRIH
                     AVADFTTLGIPGCSVIGSQTINLRVSPQPVVTITNLDTTICEGGQITLSTNIWENNVP
                     NLAYRWYTTSVDPINIIPGEMYSQLTVSPSDTTKYYVEAYQYFNEDPLVTSSCRAFDT
                     VTVNVVDAPVITAIQITQDTVCEGTQVRISAVSNSAAVGNDNYTWYRNGVVLAGITDS
                     TFTDILSAGSGITQYIYTAVLSQPSSGCISINNPSDTVYVQSAPTVAISGDAIVCTGT
                     NIQLTANLNDTIPGVTYNYQWRLNNQNVATGKLYNVSHTPNNNPYVFTVFVSAANACS
                     VLSAPFEVLVNDNPTVVVTAT"
     assembly_gap    3504..3603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3604..4157)
                     /locus_tag="PKH58_11985"
     CDS             complement(<3604..4157)
                     /locus_tag="PKH58_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89793.1"
                     /translation="MKKFTIFFLMVFMYLGNQARCETTVSAVGDDGYTCSFNSANSKT
                     YIDYSSDPLMGATGMFACNFTGSGGVSTPRYHVDGIKATKAITVRIEGAAQACNGATV
                     TLTAVIEDNDPTDTINYEYQWFIDATPIVGATDSVYVFAVSSLADMRSEFICEVTKQF
                     PGCAAVASPVFEFTKVPNSIVSITG"
BASE COUNT         1443 a          928 c          901 g         1414 t
ORIGIN      
        1 tctgtggcag ttacaactac ggtcggatta tcatttacaa gaacttcaaa tggagcagaa
       61 agtacgctgc aagcattagc ggcagaaaca aaaactgtaa agacataagg attattattt
      121 ggagtatgcg aaacattata taatttaccg gtagcaacgt tttgattatt caagcgccat
      181 tggtaattat aagtaactcc aggaatagta tcgttaagat tggcagttaa ttgaatattg
      241 gtccccgtgc aaacaatagc gtcaccggaa atagcaacgg taggagccga ttgtacgtaa
      301 actgtatcag atggattatt aatggaaata caacctgaag aaggttgtga taaaactgct
      361 gtatatatat attgtgtaat tccactaccg gctgataaaa tatcggtgaa agtggaatcg
      421 gtaattccgg caagaacgac tccgtttcta taccaagtat agttgtcatt tccaacagca
      481 gcagaattag aaactgcaga aattctaact tgcgtacctt cgcaaacggt atcttgtgtt
      541 atttgaatag ccgtgatcac aggagcatct acaacattaa cagtaacggt atcaaatgca
      601 cgacaagaag aagtaactaa aggatcttca ttaaaatatt gatatgcttc aacataatat
      661 tttgttgtat cactaggact aacagtcaat tgagaataca tctctcccgg tataatattg
      721 ataggatcaa cactggttgt gtaccatcta taagctaaat tgggaacatt attttcccaa
      781 atattggtgc tcaaagtgat ttgaccacct tcacagatag tagtgtctaa attcgtaata
      841 gttacgacag gttgcggaga aactctcaaa ttaatagttt gcgatccgat tacactgcaa
      901 ccgggtatac ccaaagtagt aaaatctgca actgcatgga ttctgtaagt gtacggatcc
      961 gcttgcggtg caatttcact tgccggtgcc gtaaaagatg cagtttcatc caaaaattct
     1021 atatcatcaa cataccaagt ataatctgca cctccaagga acatagcagc ttcgggaaaa
     1081 tcattgcaag tagcaatata ttgagcattg gatccggcac aaagatctgt cggaccggag
     1141 atgatcatat ttaagtctgg caatatgcga acattaacag atgtagccgt gatgttttca
     1201 cagccggcaa tagtagaaga aaccacaaca gtatatgcac acgttgttgg ttcatcaccc
     1261 aaattggata actcatcaat aaaagaagat gcagtatttc cttccatcaa gatgccgttt
     1321 ttgtaccatg catattgata atcggggcga gcaccgcctg caaaatcagc agaaaccaat
     1381 acttgaccat gattacaaac caactcttct ggaagagctg tccaagtagt agcatctaaa
     1441 ctattaatag ttactgcggg agtttctatc ggatcagtta ctgtaatatc tacagatgca
     1501 tttacatcgc aactcggggc agtagaagca actaactcaa aagtaaaagt cgtatcgtcg
     1561 ttgatggtaa ctgtaggatt gagagtcgtt atcacaccat ttaatttata taccggattt
     1621 tcggactgat aacctgatat attggtaaat aatgtgattt cggcacccgc gcaaacagga
     1681 gacatctccg gtgtaagcgt aacggttgga cgaggatgaa catctatagt gataacttct
     1741 gaagtggcat aacaacctct atcttcatcc accaataaat attgataagt gtgtccctga
     1801 ttggctgcag taggatgaac gtctatttgc caagtgttat cgggatctaa aaccattagt
     1861 ctgttatctt cataccaatt attataatta ttattattgt tataggtatt ctcgaaatag
     1921 taattaggat ctgtaaattg tacctcaaag gttaccgtgc cgccttcaca tatttctgta
     1981 cttccaccta tgcccaaaaa tacagtcggt tgaacagaaa ccgggatatc gacactggcc
     2041 aaacagcccg ggtgattgga aatggtaaaa ttatgggtgc cctgtgataa ggattcaatt
     2101 aattgcgaac cggtcacacc ttccaacaca acaccgttat gttgccaaat gtaattatcc
     2161 gttgcaggga cacaagtaag ttttacatta ccaccatcac aaaggaaata tatatcggca
     2221 tcagcaaggg ttgtagccaa aatggtagga gcatcagccc tcgttaaagt aatggaaccg
     2281 gtagcttgac aaccattgga tgttgccgtt acggaatagg tataggtagc atcgcttaat
     2341 gcgggagtaa gcaaagtatt accaatggcg gtgccacctg cgggggacca attataggta
     2401 atatcttcac tatcgacagc tccgacattt gccgtcaacg taacctgacc gccggcacaa
     2461 tagtttatat catcagcggt aacgactaat tgcggattac tatttacata tacgttataa
     2521 ggttctgaaa ccatagtaca tccaccggag gttacctgca aagtaaacac gtaaggtgca
     2581 gttctttgac tatgtgaagc actgtatgca gcatcagctc caacattaac tccatccaaa
     2641 aaccaaatat aggttgcacc gccaggaaca ttcgcagtta aacttacatt cgttccggaa
     2701 caaaccacgc tgttaccgga gatgctgact gcgggtgctg ccaaaacggt aacggcagcg
     2761 gaattcgctg tcgcggcaca accgctggct gcttgttcta cagtggcagt gtataaatat
     2821 tgtatattat taatattagt attggttaac tcatcggtaa atgaatttcc ggttgctccg
     2881 taaaccgtta caccatttct ttgccaagtg taaacttcac cgccggcaac accaccagca
     2941 gtaatactgg cgccgaggtc aactgtacta cctgaacaaa gtgtagtggg tgtgttcgga
     3001 gtaactgtaa gtgtcgggtc agctaccaca tgaatagtaa tgtttgctgt tccgatacaa
     3061 cctccaactg tttccacgtt taaagtgtag gtataatcac ctaccgttaa attggaaaga
     3121 gttaagttgg gttgagttcc aacatttaca acaccaagca tccaactata atttacgttt
     3181 tcgggaagga tattaccgac ataagatgtc agcgttattt ctcctcctgc gcaaacagta
     3241 gtttcagaag cgttaatggt gactgttgga ttttctacta cgcttacttc gtatggagcg
     3301 gaggtagtaa cacagccatt cgcattggta acaacaaccg tatagtaata aggattcaaa
     3361 ctggcagcta ctcctgtatt ggataaagga gcagtaagtg ctgaagctgt tgttcccacg
     3421 tttggtatta aatcgtttct ataccacaca aaatcacaag tcatatcggc gttagcagtc
     3481 aaagcaacat ctgcagttgc gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnccggtaa tggaaacaat ggagttgggg acttttgtaa attcaaagac aggtgaagca
     3661 actgccgcac aacccgggaa ttgttttgtt acttcgcaaa tgaactcgct tctcatatct
     3721 gccaaactgc ttacggcaaa tacgtaaacc gaatccgttg cgcccacaat gggagttgca
     3781 tctataaacc attgatactc atagttgatt gtatcagtag gatcattatc ttctattaca
     3841 gcagtcaaag taacggttgc accattacat gcttgagcag ctccttctat tcgaacggtg
     3901 atcgcttttg ttgctttgat accatcaaca tgataacgcg gcgttgatac gccgccggaa
     3961 ccggtaaagt tgcaagcaaa cattcctgta gcacccatta acggatcgct cgaatagtcg
     4021 atgtaagttt tactgttagc cgaattgaaa ctgcacgtgt atccatcatc accaacagcc
     4081 gaaactgttg tttcgcatcg agcttgattt cctaaataca tgaacaccat caggaaaaaa
     4141 atcgtaaatt ttttcattgc tttgaattta attattacta tatattttta tttttcttat
     4201 ctaactttta tttttcttgt ctaatttttg ttttcttaaa tgctgatatt taaaaggtta
     4261 tactattttt tttgcttatt gttacatcct cttgtaatat ccatacactt tttctcactt
     4321 tattatatca acttgcaaaa atacaaaaaa ataagtacta tcattcattt atttattttt
     4381 tttttgaact atcttatgtt tcgggggttt tggtactttt caacactttt tcaacaattt
     4441 atatttattt ataattcaga tatttacgtt ttgttttgtt atttttgttc acaattaatt
     4501 tttgaaaaat tctttatttt tttcgttctt ttgatcgccc aaagtctgat tatttaaaaa
     4561 tcgtcaactt atgagttaca cggtacttta tgaaatcatt agagcatttt tagacaaaaa
     4621 aaaggcgaaa acagttattt tcgccttatg aaataaaaag aattgatatt ctaataaaaa
     4681 attgctattt ctttttttaa ttccatcatt gccttttcgg taggcgactg ctccgcaacg
     4741 tgctgtaaaa tatttgtaat caagtctgta cctgttgctt catctc
//
LOCUS       DAOPYB010000218         4767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_22861_length_4767_cov_9.722623, whole genome shotgun sequence.
ACCESSION   DAOPYB010000218 DAOPYB010000000
VERSION     DAOPYB010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22861_length_4767_cov_9.722623"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..2371
                     /locus_tag="PKH58_11990"
     CDS             59..2371
                     /locus_tag="PKH58_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89794.1"
                     /translation="MKNLHLLRLILLMTVCFAGVTGATGQVSNVPVDTAYIYPENPAI
                     KDSVYMTYVYISSDGCPDYYLVKDSVAPGKIWVSLKKILDMGRPCITVITYFKTQITL
                     GIITRDTEVYFNGKLYKTIYVKCRLEKKGVVINCGGKLYIQEFSPLAIRQLYTFSYNS
                     LTDSNGRTYTKPAAGDTVKFSGYLSNVFPISDTVCKPVGKVICYEITGTAPQDSIPPC
                     VIDKAGIVVKCQDKLYFEDTSTPYASPVPQLYIIKGITDNSTGTDVKLKEGDRVKFGG
                     YPVMADSATGTCHIVGVAACFRLIDTTSTCIMDKKGIVEAGIDGCTSQLFIRETLTRN
                     LYSIKNAVISNSTAIKTPKPGDKVVFGGYLIRNDSSHVSLCPIQGITTCYRLIDTIPP
                     CIMDKAGTVERGTYACATMLFVRETDTQELYYIRNSSMIYADGSTIRNLSPGDKVKFG
                     AFPVSTWGKDLNLCNAVGIVNCYVITQSADTLTLSGYALAGSDTVKSGLAILFEKELR
                     KSAALSSITDGTFTFNRLHPSSYTVYVIPVRELYRNYLPTFYVDKLHYKMADFVELKQ
                     TTQDVIVKMRYYERKTGTGKIFGNINYEQYKLNDSLIVKNALSNTEGTANFNIAVNTT
                     VLLLDRQNTVIAWTLTDVNGNYAFTDIPLDTYTVVSETASASATSAVSLTSGQSIVNR
                     DLILKSNEGATGFSIPENTVLSLFPNPVKDKLSITLTSESIANVYSLSGQLHIRKQLN
                     TGSNTLDMSNMPAGIYILRIDGKSFKLIKY"
     gene            complement(2443..2898)
                     /locus_tag="PKH58_11995"
     CDS             complement(2443..2898)
                     /locus_tag="PKH58_11995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNX89795.1"
                     /translation="MNTTEEKAIHSFRSGLNCAQSVVTAYADRLKYDTIQAAGLSCGF
                     GGGMGRLQQTCGAVTGAFMVLGMYYSQRIPDQAERKNITYAAVRKLHSDFKLKCGTAD
                     CRSLLGCDLQTEEGMRFHKETNQNKTICEKCISAAVELLDEMMNDELRM"
     gene            complement(2912..4183)
                     /locus_tag="PKH58_12000"
     CDS             complement(2912..4183)
                     /locus_tag="PKH58_12000"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNX89796.1"
                     /translation="MLTQIERNEIISLIRQEVTPAIGCTEPIAVALAVAKVKELLKVV
                     PEKLDVFLSANVLKNAMGVGIPGTGRSGLPIAIAMGALIGKPEYGLEVLRDSTPEMVE
                     EGVKLVDSGMISIHLKEDIAEKLYIEVSGVAGTDRARVIISREHTRFSLIEINGNILL
                     KLADNEISETNKEQLQLSFGKVCDFAFETPVDEIRFILKTVELNKAAAVEALKGTYGH
                     SVAQTLSNGMMGHSVYNHMLSVTAAACDARMAGAMIPVMSNSGSGNQGIAATLPVSVF
                     AEETGKSEEELIRALTLSHLITIYIKQSLGRLSGLCGAVVAATGSGCGVTYLMGGTRE
                     QIGFTVKNMIGNVTGMICDGAKPSCTLKVSNGVSTATLSAMLAMDNKVVTAIEGIIDE
                     DVDKTIQNLTKIGREGMNETDKLVLNIMTNK"
BASE COUNT         1374 a         1128 c          937 g         1328 t
ORIGIN      
        1 tcatataaaa tttaaaaccg acaaatcttg ttttttgtcg gtcagtagtg aaaaaaaaat
       61 gaaaaacctt cacttgcttc gattgatttt actgatgacc gtttgttttg caggagtaac
      121 tggcgcaacc ggacaagtaa gcaatgttcc ggttgatacc gcatatattt accctgaaaa
      181 tcccgcaata aaggatagtg tatatatgac ctatgtttac atttcaagtg atggctgtcc
      241 tgactattat ctggtaaaag actctgtagc tcccggaaaa atatgggtaa gcctgaaaaa
      301 aatcctcgac atgggaagac cttgtattac tgtaattacc tattttaaga cacaaataac
      361 cttaggtatt atcacccgcg acacagaagt atatttcaac ggcaaacttt acaaaacaat
      421 ctacgtcaaa tgccggctgg agaaaaaagg ggttgtaatc aactgcggcg gtaaattata
      481 catccaggaa ttctccccgc ttgccatccg ccagctttac accttcagtt acaactcgct
      541 gaccgacagc aacggccgga cctatacgaa accggctgca ggcgatacgg tcaaattcag
      601 cggatatctc agcaatgtct ttccgatatc cgatacggtc tgcaaaccag taggaaaagt
      661 aatttgctat gaaataaccg gtacagcacc ccaggacagc ataccgccct gtgtgataga
      721 taaagccgga attgttgtga aatgtcagga taaactctat tttgaagata catccactcc
      781 gtatgcctca cctgtaccgc agctttacat tatcaaagga attaccgaca acagcaccgg
      841 caccgatgta aaactgaaag aaggcgatcg ggtgaaattc ggcggttatc cggtgatggc
      901 agattctgct acaggcacct gtcatatcgt tggtgtagct gcctgtttca ggctcattga
      961 taccacttcg acctgcatca tggacaaaaa aggaattgta gaagcaggaa ttgacggttg
     1021 cacaagccaa ctctttatcc gtgaaacact tacccgaaat ctgtattcaa tcaaaaacgc
     1081 ggttataagc aacagcactg ccatcaaaac accgaaacct ggtgacaaag tagtttttgg
     1141 tggttatctt atccggaatg actcttcaca tgtttcatta tgcccgatac agggtattac
     1201 tacctgttac cggttgattg ataccattcc gccatgtatc atggacaaag caggaactgt
     1261 agagcgggga acctatgcct gcgcaacgat gctctttgtg cgcgagactg acacgcaaga
     1321 gctttattac atccgaaaca gcagtatgat ttatgctgat ggttcgacaa tccgcaacct
     1381 tagtccgggc gataaagtga aattcggcgc gtttccggtc agtacctggg gaaaagattt
     1441 aaatctgtgc aatgcagttg gtattgtcaa ctgttatgta attacacaat ctgcagacac
     1501 actcacgctg agcggatatg cacttgccgg aagtgataca gtaaagtcag gtctggctat
     1561 tctgttcgaa aaagagctcc gcaaatctgc agcactcagc tcaattaccg acggaacttt
     1621 tacattcaac cgattgcatc cgtcatcgta cactgtttac gtcataccag tccgcgaatt
     1681 gtatcgcaat tatctgccca ctttttacgt ggataaactt cattataaaa tggcagattt
     1741 tgttgaactt aagcaaacga cgcaggatgt gattgtaaaa atgcggtact acgagcggaa
     1801 aaccggtacc ggaaaaatct ttggcaatat taactatgaa cagtataaac tcaatgacag
     1861 tctgattgta aagaacgctt tgtccaatac cgaaggaaca gcgaatttca atatcgcggt
     1921 aaatacgacc gtgttactgt tggacagaca gaatacagtt attgcctgga cactaaccga
     1981 tgtgaacggg aactacgcat ttaccgatat tccgcttgat acctataccg ttgtatccga
     2041 aactgcctcg gcatcagcca cttcagcagt gtcacttacg agtggacaaa gcatcgtcaa
     2101 ccgcgacctg atattaaaaa gcaacgaggg tgcaacagga ttttctattc cggaaaacac
     2161 agtgctatcc ctgtttccaa acccggtaaa ggacaaactc tccatcaccc tcaccagcga
     2221 atctatagca aacgtttaca gtctcagcgg acaacttcac atccgaaagc agctgaacac
     2281 aggaagcaat acactggaca tgagcaacat gccggctgga atttacattc ttcgcataga
     2341 tggcaaatcg ttcaagttga ttaaatacta atagaacttt atatcttaca aaaaaaagaa
     2401 gctgtcaaaa aggcagcttc tcgtatttct cagaaataaa acttacattc ttaattcatc
     2461 attcatcatt tcatcgagta attccacagc agcagaaata catttctcgc aaatggtttt
     2521 gttctgattg gtttctttat ggaaacgcat tccctcctcg gtttgcaggt cgcatccgag
     2581 taatgaccgg caatcggctg ttccgcattt caatttaaag tcactgtgca actttcgaac
     2641 agcagcataa gtgatgtttt tccgttctgc ctggtcgggg atgcgctggc tgtaatacat
     2701 gccaagaacc ataaacgcac ccgtcactgc accacaggtc tgctgtaatc gtcccatacc
     2761 gccgccaaat ccacaggaaa gtcctgcagc ctggattgta tcatatttca gacggtcggc
     2821 ataagcggta accaccgact gtgcgcaatt gagtcccgac cggaaggaat gaatagcttt
     2881 ttcttctgta gtgttcataa tctgcaatgt tttatttgtt tgtcatgata ttcagcacca
     2941 gtttgtcggt ttcattcatg ccttcacgac cgatttttgt caggttctga atggttttat
     3001 ccacatcttc atcgataata ccttcaatgg ctgtcaccac tttattatcc atggccagca
     3061 tcgccgacaa tgtagctgtt gaaaccccgt tggatacttt cagtgtgcaa ctgggtttgg
     3121 ctccgtcgca aatcataccg gttacattgc caatcatatt tttcaccgta aatccaattt
     3181 gttcacgcgt acctcccatc agataagtta ctccgcaacc cgaacctgta gctgccacta
     3241 cagcgccaca taatcccgac agtcgtccga gactttgctt gatatagatt gtaatgagat
     3301 gactcagggt taacgctctg ataagttctt cctccgattt tcccgtttcc tcggcaaaaa
     3361 ccgaaaccgg cagtgtggct gctatgccct ggttgccgct acccgaattg ctcatgacag
     3421 gtatcatagc tccggccata cgggcatcac aggcagcagc cgtaaccgac aacatgtggt
     3481 tataaaccga atgtcccatc attccgttgg atagtgtctg tgccacactg tgcccgtaag
     3541 ttcccttaag tgcttccaca gcagccgcct tattcaattc cactgttttc aggataaacc
     3601 ggatctcatc cacaggagtt tcgaaggcga agtcacagac cttgccgaac gacagctgca
     3661 actgctcctt gttcgtttca gaaatttcgt tatcggcaag tttgagcaat atatttccgt
     3721 tgatctcaat cagcgaaaaa cgggtatgtt cgcgactgat gattaccctt gcccgatcag
     3781 ttccggcaac tccggaaacc tcaatatata gtttttcagc aatatcctct ttcagatgta
     3841 tgcttatcat tccgctatca accagtttaa ctccctcttc caccatttcg ggtgtactgt
     3901 cgcgaagcac ttccagtccg tactccggct tccctatcaa cgcgcccata gcaatggcaa
     3961 tcggcagtcc tgacctgcct gtgccgggta ttcccacccc catggcattc ttcagcacat
     4021 ttgcacttaa aaacacatcc agtttttcag gtactacctt taacaactct ttcactttcg
     4081 caacagccaa agctacggca attggttcgg tacatccgat agcaggcgta acttcctgcc
     4141 tgatgagcga aatgatttcg ttacgttcta tttgagtgag cattctgttt tttcatttat
     4201 tgaaaacaaa agtacacatt tatctttaag aaaaatgata aaagttgttc gaaattgaaa
     4261 tcaaagacag gttatctctt gtaaatcaaa gcgcacaaaa acacacagac tccgaacccg
     4321 actatcttta atatcttgta taaatggaca tgccgggcag aataaccggc aatacgaaac
     4381 agcatttaga gcccttaaat cgagtgtctt caatattgaa gccactcatt taaccgatat
     4441 tgaccgtatt gagaaactgt tttctctggt tgccattgct tttacctggt cgtacctcgt
     4501 aggtatcttt ttgaatgaac atatgaagcc catcagggta ctcaaaaatg acagacgtgc
     4561 caaaagcttc ttcaaatatg gactggaata tattgcaatg atgcttttaa atacagaatt
     4621 tcaatcctat atagatatat ttaaattttt gtcatgtact tatattaagc ctttataatg
     4681 attttacatt gatccgtttc attgatccca ttttttggat cacccgtaaa agttgtggat
     4741 taagcatata ttttagttaa tctgaat
//
LOCUS       DAOPYB010000219         4643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_23700_length_4643_cov_22.685702, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000219 DAOPYB010000000
VERSION     DAOPYB010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23700_length_4643_cov_22.685702"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4643
                     /locus_tag="PKH58_12005"
     CDS             <1..>4643
                     /locus_tag="PKH58_12005"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89797.1"
                     /translation="NFLLAGLFVISVGLGQAQTDIQNEKPSPQTSDQNINGTTLKITG
                     HSPSINDPDYEAKKQEWIKNYPEEYKQLLESNKAAQGNVIEKPQPRITYPEKVATHAP
                     ELDDPRYDEKKQEWVKNYPEEYKSIYKPAVSNAASPNSVKLPQITFPEKVATHAPEMG
                     DVDYQAKKEAWIKNYPEEYKNLYKSANSKSENNNNAKLPQITYPAKVAAHAPEMNDPD
                     YYTKKIDWMKNYPQEYEQINKLNKKSENRPDQAESVLDYGKGGIFEGRKAQQEVVSQR
                     NETSKHFHNADGTVDAIITAGMPLNYQENGFWKTIRKEITPNSTGIYPEYDYCNTTNT
                     TKTFYSGNSAGGMLTEFGASPIREWINPQISWVVNGALTETVSIANVEATVNLNKVIY
                     SNCFSGTDVRFTQENGGKKLDIILNNSSLLSNIPDGTSHLAISETVLLPDGWSHKINN
                     SNIYIINEKGLIVFSYAAPEFYDNKKDSKKVTGNYKVEQNNNELIITTLIPIEWLTSA
                     ERIFPIIIDPTATIYATNGGWQCAACASPYIDDVNMIFVGDLDTNPGEHRGYAKFNTS
                     SINDSYSISSVTMALNCLSVGSSSATTIYSWSIDGTYGPYTAYDVSYYNDFGAGSNYN
                     SYSVSTTGLYGPTSLGSSAASTLQAQLPNDRFQVGLTNSNPSDDTYFKIFNSSSYIVV
                     TYNEATCTVCPSYDYWISPSGSWQTHSSSVDVGGCKLYEIYLYAGVEYQFSLCSADGG
                     SAAFDSYLTLYDNSCTSVASNDDYCGVQSYISYTPTTSGYYYLQNNHYSFNGGGSYTL
                     AYRYIADQCTACPSYDTYLSPTGTYQTVSGSTGMEGCDMYEVYMYAGVQYRFTFCEGG
                     GAYTGDTYLELFDYSCNSVASNDDFCGLGSQLDFTPTTSGTYLLRVRGLGGAPITYTL
                     AYKYFADVCTTCPSYDAYISPTSTYQTTSESFGMEGCHIYEAYLYANTQYRFTFCEGG
                     ASYSGDPYLELYNGSCSQVAYNDDFCSSGSQLDYTPGISGYYYLKVRGWANQPVTYTL
                     AYKYFADVCTACPSYDATITPTSVYQTTSESFGMQGCQIYQTYLTAYAQYRFTFCEGG
                     GSYTDDPVLEIYDASCNLLTYNDDYCGVGPQLDYIPTYTGTYYVKVRGFAQTASSFTL
                     AYKYICNNPCDNITPMTCGTTYTGTLGTLCSDWSTYTGCGFTEPGEEIVYSYTPPTSG
                     NFTFNALNVSGDPDYFLMSTCSNSGTNIYGNCWGVGSINVALTGGVTYYIIIDNYSSS
                     NPAQYSLSVDCCTTPSDPVVSGSTTICSGESTNISATSTNATTIYWYTASCGGTQVGT
                     SAPGANFAVSPTSTTTYYARGFNSSGSCWSASCGSATITVNSTIGTPTVTGATSICPG
                     DPTNLSATSTNASTIYWYTGSCGGTQIGTSTPGANFWVSPATTNTYYARGYNSTTGCW
                     SAACGSAAVTVNSAIGTPTVTGTASICFGESTNLSATSTNASNIYWYTSSCGGTSVGT
                     STPGANFAVSPATTTTYYARGFNSSTGCWSSGCGSVTITVTPDNTISLTAGGT"
BASE COUNT         1412 a         1043 c          924 g         1264 t
ORIGIN      
        1 aattttttgc ttgcagggtt atttgtcatt agtgtgggac tgggacaggc acaaaccgac
       61 attcaaaacg agaaaccaag tcctcaaacg tccgatcaaa atattaatgg tacaacgctg
      121 aaaattaccg gacatagccc ttcgattaat gatcctgact atgaagcaaa aaaacaagaa
      181 tggataaaaa actatcctga agaatacaaa caacttctgg aatcaaataa agctgcccag
      241 ggcaatgtca ttgagaaacc acaaccccgt ataacttatc ctgaaaaagt ggctacccat
      301 gctcccgagc tggatgatcc caggtatgat gaaaaaaagc aagaatgggt aaaaaactac
      361 ccggaagagt acaaatccat ttacaaacca gcagtttcta atgcggcaag cccaaattca
      421 gttaagctgc cacaaataac ttttcctgaa aaagttgcca ctcatgcacc tgagatggga
      481 gacgttgatt atcaggcaaa gaaggaagcc tggataaaaa attatccgga agaatataaa
      541 aatctttaca aatcggcaaa ttctaaatca gaaaacaata ataacgcaaa gctgccccag
      601 attacttacc ctgcaaaagt ggctgctcac gctccagaaa tgaacgatcc ggattactat
      661 acaaaaaaga tcgactggat gaaaaactat ccgcaagaat acgaacaaat caataagtta
      721 aataaaaaat cagaaaatag acctgaccag gcagaatctg tccttgatta tggaaaaggc
      781 ggaatatttg aaggaagaaa agctcaacag gaagttgtat cacaaagaaa tgaaacctca
      841 aaacacttcc ataatgccga cggaactgta gatgcgatca tcactgcagg gatgcctctg
      901 aattatcagg aaaatggttt ctggaaaacg attagaaaag agattacccc caatagtaca
      961 ggtatttacc ctgaatatga ttattgcaat actacaaaca caacaaaaac tttttattct
     1021 ggtaatagtg caggtggtat gctcactgaa tttggtgcat cacccataag ggaatggatt
     1081 aatcctcaaa tttcatgggt agtcaacggc gccttaaccg aaactgtaag cattgctaat
     1141 gttgaagcta cagttaattt gaataaagta atttacagca attgtttctc aggtaccgat
     1201 gtgcgtttta cacaggaaaa cggtggtaag aaactggaca tcattctgaa taattcatct
     1261 ctgttatcga atattcctga tgggacaagc catctggcca ttagtgaaac agtgctgctc
     1321 ccagatggct ggagccataa aattaacaat tcaaacatat atattattaa cgaaaaagga
     1381 ttgatagttt tttcttacgc tgctcctgaa ttttacgata ataaaaaaga ttcaaaaaaa
     1441 gtaaccggaa attacaaagt ggagcagaac aacaatgaac tgattataac cactcttatt
     1501 ccaatcgaat ggttaacatc agccgagaga atattcccca tcattattga ccctacagcc
     1561 actatctatg cgaccaatgg aggatggcaa tgcgctgcat gcgccagccc gtacattgat
     1621 gatgtcaaca tgatttttgt cggtgattta gatactaacc caggcgaaca tcgcggctat
     1681 gctaaattta acacttcctc tattaacgat tcatattcta taagcagtgt tacaatggca
     1741 ctaaactgtt tgtctgttgg aagttcttcc gctacaacca tctatagttg gtctatagat
     1801 ggcacctatg gtccctatac agcatatgac gtcagctatt acaatgattt tggtgccgga
     1861 agtaattata attcttattc ggtatccacc actggcctct atggccctac cagtcttgga
     1921 tcctccgcag catcaacact acaggcgcaa ttaccaaatg atcgctttca ggtgggactg
     1981 acaaattcaa atccttcaga cgatacatat tttaaaatct ttaatagctc tagttatata
     2041 gttgtaacat ataatgaagc gacctgcaca gtatgtccca gttacgacta ttggatttcc
     2101 ccgtctggat catggcaaac ccacagcagc tcggttgatg tgggaggttg taagttatac
     2161 gaaatatacc tgtatgcagg tgttgaatat cagtttagtc tttgttcggc tgatggaggc
     2221 tctgccgctt ttgattcgta tttaaccctt tatgataata gctgcacttc tgtagcctcc
     2281 aacgacgact attgcggagt acaatcatac atttcctata ctccgacaac ctcagggtac
     2341 tattatcttc agaataatca ttacagcttc aatggaggtg gttcttacac actggcatac
     2401 cggtatattg ctgaccagtg tactgcatgc ccatcttatg acacttacct ttctcccaca
     2461 ggcacttatc agactgtcag cggatcaaca gggatggaag gatgcgatat gtacgaagta
     2521 tatatgtacg ccggggttca gtatagattt actttctgtg aaggcggtgg agcatacacc
     2581 ggtgatacct atcttgaatt atttgattat tcttgtaatt cggtggccag taatgatgat
     2641 ttttgtggcc ttggctcgca gttagatttt actcctacca cctcggggac atatttactc
     2701 cgtgttcggg gcctgggtgg tgctcctatc acctataccc tagcctataa atactttgca
     2761 gatgtctgta cgacttgccc ctcctatgat gcgtatatat cgccaacatc tacctatcaa
     2821 accaccagcg aatcttttgg aatggaaggc tgtcatattt atgaggcata cctatatgcc
     2881 aacacgcaat atagatttac tttctgcgaa ggaggcgcca gttattcagg ggatccctac
     2941 cttgaactct acaatggatc ttgctcccag gttgcctata acgacgattt ttgcagcagt
     3001 ggatctcaat tagactacac gccaggcatt agcggatact attatcttaa agtaagaggc
     3061 tgggctaatc aaccagtaac ttacaccctt gcatacaagt attttgcaga tgtgtgcact
     3121 gcttgccctt cttatgatgc tacaattaca ccaaccagtg tttatcaaac cactagtgaa
     3181 tcattcggta tgcaaggatg tcaaatttac cagacttatc ttactgccta tgcacaatac
     3241 aggttcactt tctgcgaagg aggcggcagc tataccgatg accccgtcct tgaaatttat
     3301 gatgcatcct gcaacctgtt gacttataat gatgattatt gtggtgtggg tccgcaactt
     3361 gattatattc caacttatac aggaacatac tatgttaaag taagaggatt tgcacagaca
     3421 gcgagctctt tcactcttgc ctataaatat atttgcaaca atccctgcga taatattaca
     3481 cctatgactt gcggcaccac atataccgga accttaggca ccctttgcag cgattggtca
     3541 acttataccg gatgtggatt taccgaacca ggagaggaga tagtttactc ctatactcca
     3601 cccactagcg gaaattttac ctttaatgca ctaaatgtat caggcgatcc tgattacttc
     3661 cttatgagta cttgcagcaa ttcaggcact aatatttatg gtaattgctg gggtgttggt
     3721 tccattaatg tggctcttac tggtggcgtt acttattata ttataataga taattattca
     3781 tcgtccaatc ctgcccaata ttctctcagt gttgattgtt gtacaacacc ttcagaccca
     3841 gtagtttcag gaagcacaac catctgttcc ggagaaagca caaatatttc ggcaacatca
     3901 accaatgcca ctaccatcta ttggtacacg gccagttgcg gaggaacaca agtcggaacc
     3961 agtgctccag gggctaattt tgcggtttct cctacttcca caaccaccta ttatgcgaga
     4021 ggctttaatt cttctggcag ttgctggtct gcctcatgcg ggagcgccac gataaccgta
     4081 aattcaacaa tcggaacgcc aacggttaca ggggctacaa gtatttgtcc cggagatcct
     4141 acgaatcttt cagccacatc gacaaatgcc agcaccatct attggtacac gggcagctgc
     4201 ggaggcacac agatcggaac aagtacacca ggagctaact tctgggtttc tcctgccacc
     4261 acaaacacct attatgccag aggctataat tctactactg gctgctggtc tgccgcttgc
     4321 gggagcgccg cggtaaccgt aaattcagca atcggaacgc caacggttac agggactgca
     4381 agtatttgtt tcggagaaag tacaaatctt tcagccacat caaccaatgc tagcaacatc
     4441 tattggtaca ccagcagttg cggaggcaca tcggtcggaa caagtacacc aggagctaac
     4501 tttgcggttt ctcctgccac taccaccacc tattatgcca gaggctttaa ttctagtaca
     4561 ggctgctggt cttcaggatg cggcagtgta accatcacgg ttactcctga caataccatc
     4621 tctttaacag caggtggaac aca
//
LOCUS       DAOPYB010000220         4600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_24044_length_4600_cov_9.417602, whole genome shotgun sequence.
ACCESSION   DAOPYB010000220 DAOPYB010000000
VERSION     DAOPYB010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24044_length_4600_cov_9.417602"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1353)
                     /locus_tag="PKH58_12010"
     CDS             complement(226..1353)
                     /locus_tag="PKH58_12010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX89798.1"
                     /translation="MSWLLVLLVAFFFQLYFYLRYIAGVLRQRRRIRKGKIKFQEHQP
                     PVSVVICARDEEENLRKFLPLILEQDYPEYEVIVVNDASMDYTDDYLSLMTKQYPNLH
                     TTFVPAGATNLSTKKLGLTLGIKAARYDLLLFTDADCMPEGRNWITSMARNFNPGTEF
                     VLGYGGYLKKKGLLNTLIKYDTLFIALQYLGMAIARKPYMGVGRNLAYRSETFFRMKG
                     FASTLHLQSGDDDLMVNRGATERNTAVEISSESVTWSVPKNTFKQWFIQKERHLSVSN
                     QYNTTSKFSLIQEPLSRAVFYFAIIFTAITGNWISLAAAGFIFLLRYTMQAIIINKSA
                     KQLGEQRFFLSILLLDILLPLITLYILVFGRTKRKKGMKWK"
     gene            complement(1450..2607)
                     /gene="lysA"
                     /locus_tag="PKH58_12015"
     CDS             complement(1450..2607)
                     /gene="lysA"
                     /locus_tag="PKH58_12015"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNX89799.1"
                     /translation="MLKAQYPLEKFRNIPTPFYYYDLDVLRDTLKEINEQTTGYNFEV
                     HYALKANVNPEVLNIIKEAGLGADCVSGGEVQAAVNAGIPSSKIVFAGVGKADWEINL
                     ALENDIFCFNAESIPELEVINELAAAKNKTASVALRINPNVDAHTHNYITTGLNENKF
                     GINMEDIDKVISVLPDLRNIRMIGIHFHIGSQITDMTAYHNLCVRISEIEDIFASKNI
                     FLQHINVGGGLGINYEHPNHFPVPEFETYFRTFKEHFKLQPGQTLHFELGRSVVAQCG
                     SLISKVLYVKEGSTKNFAILDAGFTDLIRPALYQAYHRIENLSSELPVEKYDVVGPIC
                     ESSDVFDKDCYLNKTRRGDIIAIRSAGAYGEIMASQYNLRALPKAYTSDNI"
     gene            complement(2670..3983)
                     /locus_tag="PKH58_12020"
     CDS             complement(2670..3983)
                     /locus_tag="PKH58_12020"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNX89800.1"
                     /translation="MQILKFGGTSVGSAARMKDVAKLISDGKQKIVVLSAMSGTTNYL
                     VEIADYFYKGNTSGALEKINLLEHKYNEVIQELYSTPEKASEAEKIILSHFDFLRSFS
                     KSVFTLFEEKAILAQGELLSTAMFQLYLEEAGKSSILIPALEYMRIDKNAEPDAAYIA
                     ENLKKILSENPEKEIYITQGYICRNFYGEIDNLQRGGSDYSASLIGAAVKAEEIQIWT
                     DIDGMHNNDPRIVQGTKPVPYLHFEEAAELAYFGAKILHPTCILPAKLNNIPVRLLNT
                     MDPEAPGTLISNKSRKGVIEAVAAKDGITAIKIKSGRMLLAHGFLRKVFEIFESNQTS
                     IDMITTSEVGVSVSIDNSKRLEEIVNDLKKFGTVTVDKDMVIICVVGDLPYETVGFQA
                     KVVDALKDIPIRMISYGGSNYNISILVKSEDKNRALNQLSQALFN"
BASE COUNT         1279 a          929 c          962 g         1430 t
ORIGIN      
        1 ccgaaggtcg cgggttcgaa ccccgtttgc cgctccagtc tggtctggaa ttaatcctga
       61 atttcacata ctatgccccg gggtgaattt acgaaactta gatttcaacg tcctgatgta
      121 tagcccaata aaaaggcttc cttgagcaag ggagcctttt ttgtgttgaa ttttgtaaga
      181 tgattgtaaa agaaatgtca gcttaacggt tttatcagca atgccttatt tccacttcat
      241 accctttttc ctttttgttc tgccgaatac cagtatatat agtgtaataa gtggcagaag
      301 tatatccagc agcaagatgg aaaggaagaa acgttgttca cccagctgtt tggccgattt
      361 gttgatgatg attgcctgca tggtataacg aagcagaaaa ataaatccgg ctgctgccag
      421 gcttatccag ttgccggtta tcgcagtaaa gataattgca aagtaaaata ctgcacggct
      481 tagaggttcc tgtataagac tgaatttgct ggtggtattg tattgattcg aaaccgacag
      541 atggcgttcc ttttgaataa accactgttt gaacgtgttt tttggtaccg accaggtaac
      601 gctttctgac gaaatctcta ccgcggtatt tctttccgtt gccccccggt ttaccatcag
      661 gtcgtcgtct cccgactgaa gatgcagtgt ggaggcaaat cctttcatcc tgaaaaatgt
      721 ttcgctgcgg taagccagat tgcgtcctac ccccatataa ggcttacggg caatggccat
      781 tcccagatac tgaagggcga taaataaagt gtcgtatttg attagcgtat tcagcaaccc
      841 ctttttcttg aggtatccgc cgtaacccag cacaaactct gttccgggat tgaaatttct
      901 ggccatcgaa gttatccagt ttctaccttc aggcatgcaa tctgcatcag taaataacag
      961 caggtcgtat ctggctgctt taataccaag cgtaagtccc agctttttgg tgctcaggtt
     1021 ggtggctccg gctggaacaa aggtggtgtg caggttcggg tactgtttgg tcatcaatga
     1081 gaggtaatcg tccgtgtagt ccatcgacgc atcgtttaca acaataacct catactccgg
     1141 ataatcctgt tcaagtatca gcggtaaaaa tttgcgaaga ttttcctctt cgtcacgggc
     1201 acaaattacc accgaaacag gcggctgatg ttcctgaaat ttaatttttc ccttcctgat
     1261 gcgtcggcgt tgacgcagta cgccggcaat gtaacgcaga taaaagtata gctgaaagaa
     1321 gaaagcgaca agcaatacca gtaaccaact caattcaata tacgtaaaat gtatttcacc
     1381 aatattcata tcctgaaaaa acagtaattc cggttaagtg attaaaaatg tgaaaaactg
     1441 cactaaaaat cagatattat ccgatgtata ggctttgggc agtgcgcgca ggttgtattg
     1501 cgatgccatg atttcgccat aagctcctgc cgaccggatg gcaataatgt caccccgacg
     1561 ggttttgttc aggtagcagt ctttatcgaa aacatcggac gattcgcaga taggacctac
     1621 cacatcatac ttttctactg gtaattctga actcagattt tctatccggt gataagcctg
     1681 atagagcgca ggacgaatta aatccgtaaa acctgcatcg aggattgcaa aattcttggt
     1741 ggaaccttct ttcacgtaaa gcactttcga aatgaggctt ccgcactgag ccaccaccga
     1801 acggccgagc tcaaagtgca gggtttgacc cggctgtagt ttgaaatgtt ccttgaaagt
     1861 tctgaaatag gtttcaaact cgggaacggg gaaatggttg gggtgttcgt agttgatacc
     1921 cagaccgccg cctacgttga tgtgctggag aaatatgttt ttcgaagcaa aaatatcttc
     1981 aatttccgaa atacgtacac acaggttgtg gtaagcggtc atgtcggtaa tctgtgagcc
     2041 aatatggaaa tggatgccaa tcatccggat attccgcagg tcaggcaata cgctgataac
     2101 cttgtcgata tcctccatgt tgatgccgaa tttattttca ttcagcccgg tggtgatgta
     2161 gttgtgtgta tgggcatcca cattcggatt aatgcgcaga gcgacagagg cagtcttgtt
     2221 tttagcagca gccagctcgt tgattacttc cagttcaggt atagattctg cattgaagca
     2281 gaaaatatcg ttttcaagcg ccagattaat ttcccaatca gctttaccca ctccggcaaa
     2341 tacaatcttg gacgatggaa ttccggcatt aacggcagcc tgcacttcgc cgccacttac
     2401 acagtcggca ccaagtccgg cttctttgat tatattcagc acttccggat tgacattagc
     2461 tttcagcgca taatgtacct caaagttgta acctgtggtt tgttcattaa tttccttcaa
     2521 tgtgtcgcga agaacatcca gatcatagta ataaaatggt gtagggatgt tcctgaattt
     2581 ttctagggga tattgagctt ttaacataga tttctgaaat ttgtttctta aaaaaccgat
     2641 aagtttttgt cttaccggtt tgttagtttt taattgaata atgcctgact taactggtta
     2701 agtgccctgt ttttatcttc cgattttacc aggatggaga tgttgtaatt acttcctccg
     2761 taagatatca tacggatagg aatatctttc agcgcatcta ctacttttgc ctggaaacca
     2821 accgtttcgt aaggcaggtc gcctacaacg cagatgatga ccatatcttt gtcaacggtt
     2881 acagtaccga atttcttcag atcgtttacg atttcctcca gtcgtttgct gttgtctatt
     2941 gatactgaca cacccacttc agaagtcgtg atcatatcaa tggatgtctg attgctttca
     3001 aatatttcaa aaactttgcg caggaatcca tgtgccagca gcatacgtcc cgatttaatc
     3061 ttgatggctg taataccgtc tttggcagca acagcttcaa tcaccccctt gcgcgattta
     3121 ttggaaataa gagtgccggg tgcttccgga tccatggtgt taagtaacct aaccggaata
     3181 ttattgagtt tggcgggcag tatgcaggtc ggatgtagaa tttttgcacc gaaataagcc
     3241 agctcggcag cttcttcaaa atgaagataa ggaaccggtt ttgtgccctg tactatgcgt
     3301 gggtcattgt tgtgcatacc gtcaatatca gtccatatct gaatttcctc ggctttcacg
     3361 gctgctccaa tcaatgaagc agagtaatcg cttccaccgc gttgcaggtt gtcaatttcg
     3421 ccatagaagt tacggcagat gtatccctgt gtgatataga tttccttttc tggattttcc
     3481 gacaggattt ttttcagatt ttcggcaata taagctgcgt ccggttctgc atttttatca
     3541 attcgcatgt attcaagtgc agggataaga atgctgcttt taccggcttc ttccagataa
     3601 agctggaaca tggcggtcga tagcaattcg ccctgagcca ggattgcttt ttcttcgaat
     3661 aatgtaaata cgcttttaga gaaagaacgc aaaaaatcga aatgggacag gattattttt
     3721 tcagcttctg aagctttttc cggagtggaa taaagttcct gtatcacttc gttgtattta
     3781 tgttccagca gattgatttt ttccaacgca ccggatgtat tacctttgta aaaataatcg
     3841 gctatctcta ccagatagtt tgttgtgcct gacatagccg atagtacaac aattttttgt
     3901 tttccatcgc tgattagttt ggccacatct ttcatacggg ctgccgaacc tacggaagtt
     3961 ccgccgaatt taagtatttg catgttataa gatattttat tgttgataaa aatacggggc
     4021 aaagatacta tttttttctg taaggtttta gtatcaaaaa aatagaatta ttccttctaa
     4081 aatatactaa ttctgatttt tgcttaaaag tctgtaaaaa ttaacaacat ttaaataaaa
     4141 cctaattttg ttttttttta taaatttgct gaatattaac ttaaaaaaag gaattgctat
     4201 tctttgaaac atttttatga ataatatagg ctgttaaagt gtaacctaat taaaatttat
     4261 atgaaaattt atttaaggtt tattttgttt actttattga caactttttg ttcgtgtaca
     4321 caagagctgt atcttgacga gataaataaa tcatatctat tctcaataca ggatgcaaaa
     4381 caatggtatg aaacacagct tctaactaaa agtattagat ctcaacaaaa agtaaaagga
     4441 gagggtatgg atattcaaat tcaaccccac ttaagttggg agaaagcagt tcttggcaat
     4501 gattcccttt ggactgttgt agaactgcct tgggaatatt taaacatatg tgtatgtatc
     4561 acagtccgta tgttgcaaac aaaaatttat tagggaaaat
//
LOCUS       DAOPYB010000221         4589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_24134_length_4589_cov_10.558227, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000221 DAOPYB010000000
VERSION     DAOPYB010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24134_length_4589_cov_10.558227"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..4471
                     /locus_tag="PKH58_12025"
     CDS             218..4471
                     /locus_tag="PKH58_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HNX89801.1"
                     /translation="MKALFSSVILMFVLFISFAENEPNNSITEANPISINSTSNGHIG
                     GGVYPSIDNQDWWVVTLTTQGSLTITTNSGSEVCVVSYLYLADGTTILADTWSGPCSD
                     TNRTMNVPNLAPGNYYIKATNYSGAGNYTISTTFTSSSFPSDIEPNNYMNQAVQIQPT
                     DTAVGILGYIGQSYQDDNDWYKITLVEDGSLTLRVLTNQTLTVNTLYLFDSDTNTIIA
                     SGNWGTDATVNHPNLSPGSYYINLSHYTGYGGYQFISTFVPAYYPNDNLTNDQIENAQ
                     VIGINDSLTGRLGYYNNGYTDISDYYKFTIQEDGKIRFHVVTTQSLYTGDLLIFDTDT
                     NTLIINGSWGQDAQVVYNNISPGIYYLKVPRYTGYGSYYLTSTFTPATYPNDQELNDI
                     IQNSIPILPNDSLTGRLGYYGSGHTDIADYYHFTTPVDGKIRFRVVTTDILQTNNLLI
                     FDSDSLSILISGNWGIDAFVEYPNLAAGDYFIKVPRYTGYGSYYITATFTPAVFSTDT
                     EPNNIAQNSMLLNIGDTVTGHLGFYGNDIFDGSDWYHFIVTERGKVTFNALGVGPLQF
                     NNLLIFGTDTTSLLGYGSWGTSGSVNTIITEPGTYFFKLNQYTGYGSYQLTSEFKPVP
                     VADYSFIQNLHTVSFTNHTTGADQYTWNFGDGITSNLVSPTHTFTQPGEYFVELIAQN
                     ECGPDTMIKQITILGVQGINGNKGGNNGIASFYIYGGGLTANATVTLKRSGYADIPAD
                     TVIKPNVFSIKASFDLTGVAVGVWNVEVVVPGFPPYTLNNCYTVEPIVEPEFDLYVSG
                     RNKILFNRWQTYTINYSNLGNVDMIGVPFFLLISDIPGMDIQFLSKQIGMPAPPNNIW
                     WQQLQDSTETYFTIDSLDGEPFAARVYPLFIPNIPASYSGSFTIRIKTPSDIRILTWV
                     NDPYATGSKLNDYQTCVMWAQATALANGIVNILGTLVPGAQCVSSVSQTLYGLTYNET
                     SLGSGLHAIANAGLSCAFDIGRNLPVIKAWELSYGIYSLINDVYSNYSAVEECKRKFP
                     KDKPTDNPIHAVSSFDPNEIIGVKGFKNEHYTGKETPYNYQIYFENMATASAPAQEVF
                     VYDTLDLTKYNKEQFNFGNVGFGDTILYPIQNGYEFSLDVDLRPNKNLITRISGVFDT
                     ITGIISWSFVSLDPLTMDLTEDPMGGFLPPNINYPEGVGFVSFNIGVNESLTHGTIID
                     NQATIIFDLNPQILTNIWSNEIDLDPPSSALNPLESTIYDTTFLVSWNGNDNESGLLN
                     YSIFVSKNSGPVYEWISKTNKTEAWFVGEFGNSYSFFTQAIDSVGNIEQIYSIPDETV
                     WLEPSGIVLYEPIHLAKVYPNPCQKYITVDVTLIFSESLTIEIYNSIGNQIFINTYPN
                     LSEGEQHINIDIRTFSNGVYTLKIKGESYQKVFKIVKVD"
BASE COUNT         1499 a          828 c          813 g         1449 t
ORIGIN      
        1 tagaggtttc taatttattc aatgtagaat attttgatta tccgaacatc atagctccag
       61 gtagatgggt aaaagcgggt atttcattac aattatagat caaaaaaagt aaataaaaaa
      121 taacttgact tatatttaaa gttattgtat atttggacat taaaaacaaa aaacgggaat
      181 catgactaat tcccgttttt attaaagaca aacgattatg aaagcgttat tttcctccgt
      241 aattttaatg tttgtactat ttatctcatt tgctgaaaat gagcccaata acagtataac
      301 agaagccaat cctatttcta ttaacagtac atcaaatggg catattgggg gtggtgtgta
      361 tccaagtatt gataatcagg attggtgggt ggttacctta acgacccaag gcagtttaac
      421 tataaccaca aattcaggct ccgaagtttg tgttgtttcc tacctgtatc tggcagatgg
      481 aacaaccatt ctagcagata cctggtcagg tccctgttct gatacaaata ggaccatgaa
      541 tgttcccaat cttgcaccag ggaactatta catcaaagca accaattatt caggagccgg
      601 aaactatacc atttcaacta catttacatc cagctcattc ccaagtgaca ttgagccaaa
      661 taattacatg aatcaagcag ttcaaattca gccgacagat acagcggttg ggatattagg
      721 gtacattgga caaagctatc aagatgacaa tgattggtat aaaattacgt tggttgagga
      781 tggaagtttg actttgagag tgcttacgaa tcagacatta accgttaata cactttatct
      841 ttttgattca gatactaata cgatcattgc aagtggcaac tggggaactg atgcaacggt
      901 aaatcatccc aatttgtctc ccggttcgta ctatatcaat ctctcacatt atacaggata
      961 tggcggttat cagttcattt caacatttgt tccggcgtat tatccgaatg ataatcttac
     1021 aaacgatcaa attgaaaatg cacaagttat tggtataaat gattcattaa caggtcgttt
     1081 aggttattac aataatggtt atacagatat ttctgattat tataaattta cgattcagga
     1141 agatggaaaa atcagatttc atgtcgttac tacacaatct ttatatacag gtgatttact
     1201 tatttttgat acggatacta acacattgat aattaatggt tcctggggac aggatgctca
     1261 agttgtttac aataatatat ctccgggaat ttattaccta aaagttccaa gatataccgg
     1321 ttatggctcc tattatctta cttccacatt tactccggca acctatccaa atgatcagga
     1381 acttaatgat atcattcaga acagtatccc catacttcca aatgattcac tcaccggtcg
     1441 gctcggttat tatggcagtg gtcacaccga tattgcagat tattatcact ttacaacacc
     1501 cgttgatgga aaaatccgat ttagagttgt tacgactgat attttacaaa caaataattt
     1561 actgattttt gatagtgatt ctttaagtat cctaatttca ggaaattggg gaatagatgc
     1621 atttgttgaa tatccaaatc tggcagcagg agattatttt attaaagtcc ctagatatac
     1681 cggatacgga tcttattata tcactgctac atttacacct gcagtctttt ccaccgatac
     1741 agaacccaat aatattgctc aaaacagcat gctcctcaat attggcgata cagttaccgg
     1801 tcatctggga ttttatggaa acgatatttt tgatggttcc gattggtatc actttatagt
     1861 aactgaacgg ggtaaggtaa cctttaatgc attgggtgtc ggacctttac aattcaacaa
     1921 ccttttaatc tttggtacag atacgacttc ccttctcgga tatggatctt ggggaaccag
     1981 cggaagtgtc aatacaatca tcactgaacc gggtacctat ttctttaaac tgaatcaata
     2041 taccggttat ggatcttacc aattaacttc ggaattcaaa ccggttccgg tagctgatta
     2101 ttcatttatt caaaatctac atactgtaag ttttacaaat catacaaccg gtgctgatca
     2161 atacacctgg aattttggag atggaattac atcaaatttg gtaagtccca cccatacttt
     2221 cactcaaccg ggagagtatt ttgtggaatt gattgctcaa aacgaatgtg gaccagatac
     2281 tatgatcaag cagatcacca ttttaggcgt tcagggaatc aatggaaata aaggaggaaa
     2341 taatggaatt gcatcctttt atatttatgg aggtgggtta actgccaacg ccacagtaac
     2401 tttgaaaaga tcaggatatg cagatatacc agctgatacg gtcataaaac caaatgtatt
     2461 ttcgatcaaa gcgtcattcg acttaacggg tgttgccgtt ggagtttgga atgtagaagt
     2521 agtagtaccc ggatttccac cttatacatt aaataactgt tatacagtgg aaccgatagt
     2581 agagcctgaa tttgatttat atgtttctgg acggaataaa attttattta acagatggca
     2641 aacctatact attaactaca gcaacctggg taatgtggat atgattggag tcccgttttt
     2701 cctattaatt tctgatattc cgggcatgga tattcaattt ttatccaaac aaattggaat
     2761 gccggcacct ccaaacaata tctggtggca acaattacaa gactcaacag aaacctactt
     2821 tactattgat tccttagatg gtgagccttt tgcagcaaga gtgtatccat tgtttattcc
     2881 aaatattccg gcatcatatt ccggtagctt tacaattcga atcaaaactc cttcagatat
     2941 tagaatctta acctgggtaa atgatcctta cgcaaccgga agtaaactta atgattatca
     3001 gacttgtgta atgtgggcac aagcaacagc tttagctaat ggaattgtga atattttagg
     3061 aacattagtc cccggagccc aatgtgtttc cagtgtctca caaactttat atggattaac
     3121 ctataatgag acaagccttg gcagcggatt acatgctatt gccaatgccg gtttgagttg
     3181 tgcttttgat attggacgca atcttcctgt cattaaagcc tgggaacttt cctacggaat
     3241 ctattcattg atcaatgatg tatattccaa ttattctgct gttgaagagt gcaaaagaaa
     3301 atttccgaaa gacaaaccta cagacaaccc catacatgca gtttcctctt ttgaccccaa
     3361 tgaaatcatt ggagttaaag gatttaaaaa cgaacactat acaggtaaag aaactccata
     3421 caactaccag atttactttg aaaacatggc tactgcttct gctcccgctc aggaagtgtt
     3481 tgtttatgat acacttgatc tcacaaaata caataaagaa caattcaact ttggaaatgt
     3541 gggtttcgga gacactattt tatatcctat tcaaaacgga tatgagttct ctttggatgt
     3601 agatttaaga ccaaacaaaa atttaatcac gagaatttcg ggtgtttttg atactattac
     3661 cgggatcatt tcctggagtt ttgtctctct tgacccttta actatggact taacagaaga
     3721 tcctatgggt ggttttcttc ctcccaacat caattatcca gaaggggttg gatttgtatc
     3781 tttcaacatt ggggtcaatg aaagtttaac acatggaacc attatcgaca atcaggcaac
     3841 gatcattttt gacttaaatc ctcaaatact gactaatatc tggagtaatg aaatagatct
     3901 tgatcctcca tccagtgctt taaatcctct tgaaagcacc atttatgata ccactttttt
     3961 agtctcttgg aatggaaatg ataacgaaag tggattattg aattactcca tatttgtgtc
     4021 taaaaatagt ggacctgtgt atgaatggat ctccaaaacc aacaaaacag aagcgtggtt
     4081 tgttggtgaa tttggcaatt cctatagctt tttcactcaa gctattgatt ctgtggggaa
     4141 tattgaacaa atttattcca taccggatga aactgtgtgg ttagaaccta gcggtatcgt
     4201 tttatacgaa cctatccatt tagccaaagt atatccaaat ccttgtcaaa aatatattac
     4261 agttgatgta accctgattt tttcagaaag tttaactata gaaatttata attctattgg
     4321 aaatcaaatt tttataaata cttatccaaa cttatctgaa ggagaacaac atataaatat
     4381 tgatatcagg acattcagta atggggttta tacattaaaa atcaaaggag aaagttatca
     4441 gaaggtcttt aagatcgtta aggtagatta aactttgaga gaagttaata gtcaaaatag
     4501 aacttgaaaa caaacaaaaa ataaccaaaa acaaaataaa ctatgaaaat attagcattt
     4561 tccacaagcg gaagttccac ttccatcaa
//
LOCUS       DAOPYB010000222         4585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_24164_length_4585_cov_10.096468, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000222 DAOPYB010000000
VERSION     DAOPYB010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24164_length_4585_cov_10.096468"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4138)
                     /locus_tag="PKH58_12030"
     CDS             complement(<1..4138)
                     /locus_tag="PKH58_12030"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNX89802.1"
                     /translation="MKKKSLLFILFILLLCLAATNVKAQLGCAWALNPQAVSPLGGQI
                     WGQSIKTDAAGNSYITGWFYNTADFDPGAGTAALTSAGNSDIFIAKYDAAGNYIYAKR
                     MGGGSNDYTYSIDIDAAGNTYITGYFYGTADFDPGAGTAYLTAAGNNDIYFAKYDAAG
                     NYVYAKSIGGTGSDIGRSIVIDAAGNAYITGNFQLTADFDPGIGTANLTSAGSVSVFL
                     AKYDASGNYLYAKNIGGGSAWGYSIAIDASGNAYITGNFLGTVDFDPGAGAVNLSSSG
                     GYDIFIAKYDASGNYIYAKTMGGNGADYGHNIAIDASGNAYLIGFFESTVDFDPGPGV
                     VSLTSAGANDIFFARYDASGNFVYVKQIGGTGYDNGYSIAIDAAENAYITGFYQGTVD
                     FDPGVVTANLTAAGSYEIFVAKYDASGNYIYAKSIGGTSTDIGNSIAIDASGNAYVTG
                     YFQNTADFDPGTGTANLSIISGNDNAFIAKYNTNGAYVWAGAIGGYSNTSCLQAANCV
                     KVDNAGNSYMTGYFLGSIDFDPGIGTAILTSVGGWDIFIAKYDVSGNFIYAKGIAGSS
                     YEVGNSIAVDATGNVYITGYYQGTVDFDPGAGIANLTPAGQDIYFAKYDAAGNYVYAK
                     SLGGADNDQGFSIAVDVAGNVYITGYFRGTADFDPGAGTANLTSAAENDIFFAKYDAG
                     GNYVYAKSIGGSSQDIGNSLAVDAAGNVYLTGGFYYTVDFNPGAGTDNLTASDRDIFI
                     AKYDATGNYVFAKSMGGPSIDNGNSIAVDASGNVYITGLFYSTADFDPGPGTVNHTSS
                     GQSDIFIAKYDPSGNFIYANGMGGAGSDIGNSVSLDASGNAYFTGYYSGVVDFDPGAG
                     TAFLTSPSRDIFLAEYSASGNYVYAKNMGGAGSDAGTSVCVDAGGNVYVTGSFYYLAD
                     FDEGITLQSINNGDIFLAKYVPSPIVWTGAVNTDWDTPGNWSGNTVPTSALDAFIPLV
                     ANLPAVNSVNSKCRNITINPGASVTVNSGKNLSLYRNWTNNGLSDAGSGTVTFIGSTA
                     QSINGNTSFGNLTINNPSGVSLFGNTELTGVLTPALGTLASNGYLTLASNTSQTAIIS
                     GTGFGSVYGNVTMQRYMPSKLGYHYYSSPFTAAPVNEFVDEIGTITSGDPYGGTNDTL
                     QTVTPFPNFYAYDETPQFFLQIGWTGAGATMTPMRGYCINFGAAGGALTTDVTGMVNN
                     GPLSYPVTKTASPYPTADGWNLVGNPYPSPIDWQAASGWTKTNVSDGIYYFKPSTQYT
                     GSYSTFVNGIGANGGTAIIPSMQGFFVKATGAGTLGVTNAVRVNSQPTFFKSTTVTSN
                     PLLRLKGYPAANEPSGDETVIYFDTQATNVFDNDLDAYKWMNNDPAFPNLFTRDDSPS
                     S"
BASE COUNT         1333 a         1057 c          917 g         1278 t
ORIGIN      
        1 aagaactcgg tgagtcgtcc ctggtaaata aattggggaa agccggatcg ttgttcatcc
       61 acttgtaggc atctaaatca ttatcaaaca cattggtggc ctgggtatcg aaataaatta
      121 ctgtttcatc ccccgaaggc tcgttggctg ccggataacc tttaagcctt aataaaggat
      181 ttgaggtaac tgttgtggac ttaaaaaatg ttggctgtga attaaccctc accgcgttgg
      241 tcacacccag agttccggcc ccggtagctt ttacgaagaa cccctgcatg gaaggaatga
      301 ttgctgtccc accgttagca ccaatcccgt tcacaaacgt actgtatgat cctgtatatt
      361 gggtgctggg tttgaaataa tagataccat cgcttacatt ggtttttgtc catcctgatg
      421 ccgcctgcca gtcaataggc gaggggtaag ggtttccgac aaggttccat ccatcggcag
      481 tagggtaggg tgaagccgtt tttgttacag gataacttaa tggcccgttg ttaaccatgc
      541 cggtaacatc cgtggttaaa gcgcctccgg cggctccgaa attaatgcaa tagcctctca
      601 taggcgtcat cgtggctcct gcgccggtcc agccgatctg caaaaaaaac tgaggtgttt
      661 catcatatgc ataaaaattg gggaaaggag ttaccgtctg cagtgtatcg ttagtcccgc
      721 cataaggatc accagatgtt attgtcccta tctcatcaac aaactcattg acaggtgctg
      781 ctgtgaacgg cgaggaataa taatgatagc cgagtttgct gggcatgtaa cgctgcatgg
      841 ttacattgcc gtatacgcta ccgaagcctg taccggaaat aatagctgtc tgtgaagtat
      901 ttgaggccaa ggtcaggtag ccattgctgg ctaaagtgcc aagtgcggga gttaaaactc
      961 ctgtcagttc agtattgcca aataagctga cgccagacgg attatttatg gtaagattcc
     1021 cgaatgaggt gttgccgttt atggattgcg ctgtactgcc tataaaagtt accgttccgc
     1081 ttccggcatc gctgagtccg ttatttgtcc aattgcgata cagggataaa tttttaccgc
     1141 tgttaacggt aacagatgca cctggattaa ttgtaatatt acggcacttt gaatttactg
     1201 aattcacggc tggcagattt gcaactaaag gtatgaaagc atctaaagca cttgttggca
     1261 ctgtgttgcc tgaccagttc cctggtgtgt cccagtcagt attcactgct cctgtccaga
     1321 ctataggtga tggaacgtat tttgccaaaa aaatatcacc attatttatt gattgcaagg
     1381 tgataccctc atcaaaatct gccaggtaat aaaagctacc agtaacatat acattacctc
     1441 ctgcatcaac gcaaacagaa gttccagcat cactcccagc ccctcccatg tttttagcat
     1501 aaacataatt accactagca ctgtattcag ccagaaaaat atccctactg ggtgaagtca
     1561 ggaatgctgt tccggccccg gggtcaaaat cgacaacgcc actatagtaa cctgtgaaat
     1621 aagcattacc tgaggcgtca agagaaacag aatttccaat atcacttcca gccccgccca
     1681 taccattggc ataaataaaa tttcctgaag gatcatattt tgcaataaaa atatcacttt
     1741 gacctgaaga tgtatggttt actgtcccgg ggcccggatc aaaatcggca gtgctataaa
     1801 acaggcctgt gatataaaca ttacctgagg catcaacagc aatggaattt ccgttatcaa
     1861 tagacggtcc tcccatactt tttgcaaaga cataatttcc ggtggcatcg tattttgcaa
     1921 taaaaatatc ccgatcgctg gcagtcagat tatctgttcc cgcgcccggg ttaaaatcaa
     1981 cggtataata aaaacctcct gttaaataca cgttacctgc ggcgtcaacg gcaagggaat
     2041 ttcctatatc ctgggaagac ccaccgatgc ttttagcata aacataattt ccgccagcat
     2101 cgtatttggc aaagaagata tcattttctg cggctgaagt cagattagcc gtcccggcac
     2161 ccgggtcaaa atcggctgta cccctaaaat atccggttat atatacattc cctgccacgt
     2221 cgacagcgat cgaaaacccc tgatcattat cagccccacc caaacttttt gcataaacat
     2281 aattgccggc agcatcgtat ttggcgaaat aaatatcctg tcctgcaggg gtcaggtttg
     2341 ctatcccggc acccggatca aaatcgacgg tgccctgata ataccctgtt atatatacat
     2401 tcccggtagc atcaaccgca atggaatttc caacttcata actggatccg gctatacctt
     2461 ttgcatagat aaaatttccg gaaacatcat attttgcaat aaaaatatcc caaccgccaa
     2521 cagaagtcaa aatggctgtt ccgatgccag ggtcaaaatc aatggagccc agaaaatacc
     2581 cggtcatata tgaattacct gcattgtcca ctttaacaca attagcagcc tgtaaacagg
     2641 atgtgtttga ataaccgccg atagcacctg cccatacgta ggcgccattg gtattatatt
     2701 ttgctatgaa agcattatcg ttgccactaa taatacttag gtttgctgtt cctgtaccag
     2761 gatcgaaatc tgcagtgttc tgaaaatatc cggtaacata cgcattaccg cttgcgtcaa
     2821 tagcaataga atttccaata tctgtagaag ttcctcctat gctttttgca taaatataat
     2881 ttccggaagc atcgtatttt gctacaaaaa tttcataact acccgcggcg gtcaggtttg
     2941 ctgttacgac acccggatca aaatcaacgg tgccctgata aaatcctgtg atatatgcat
     3001 tctctgcagc atcaatagca atagaatatc cattatcata accagtgcct ccgatctgtt
     3061 ttacataaac aaaatttccg gaagcgtcat atcttgcaaa aaaaatgtca ttagcgcccg
     3121 cggaagtcag gcttaccact cccgggccag gatcaaaatc gacagtgctc tcaaaaaatc
     3181 ctatcagata ggcattccct gaggcgtcaa tagcaatatt atgtccataa tcagccccgt
     3241 taccacccat ggttttcgca taaatataat ttccggaagc atcgtatttt gcaataaaaa
     3301 tatcataacc gcccgatgag ctaagattta ctgcccctgc cccgggatca aaatcgacag
     3361 tgccaagaaa attgccagtt atatatgcat tacctgaagc gtcgatggca attgaatatc
     3421 cccaggcaga cccgccccca atatttttgg cgtaaaggta attcccggaa gcatcgtatt
     3481 ttgcaagaaa aacactcaca ctgccggctg aggttaggtt agcagttcct attcccggat
     3541 cgaaatcagc agttaactgg aagttgcctg ttatataagc attccctgct gcatcaatta
     3601 caatcgaacg gccaatatct gaccctgtcc cgccaatact tttggcgtaa acataatttc
     3661 cggcagcatc gtatttggcg aaataaatgt cgttattacc ggccgcggtc aggtatgctg
     3721 tcccggcacc cgggtcaaaa tcggcggtgc cataaaaata tcctgtaata tatgtgttac
     3781 ctgctgcgtc aatatctatg gaatatgtat aatcattcga tccacccccc attcttttgg
     3841 cataaatgta atttccggca gcatcgtatt ttgctataaa aatatcagaa ttgccagcag
     3901 aggtgagggc tgctgtcccg gcacccgggt caaaatcagc agtattatag aaccatccgg
     3961 ttatataact gttacctgct gcatctgttt ttattgattg tccccaaatt tgtccgccca
     4021 atggcgaaac tgcctgaggg ttcaaggccc aggcacagcc taattgtgct tttacattcg
     4081 ttgctgctaa acaaagcaaa aggataaaaa ggataaaaag gagagatttt tttttcattt
     4141 tagtattatt taaattgttc attaaaaatc tttcttgagc ctactcagaa aagtatgatt
     4201 tttgaaaatc agctttgccc cggcccaaag ggaggctgat ttcctgcgac ttccctttgg
     4261 gattacggca aaaagttgca ggataataaa aacggggctt ttcggattgg actcatagtt
     4321 agattcaaaa taaaaaaaat tgatttaatt aaaatgatgt caatatattg gtcaaacata
     4381 acaacccaac tcgtatccgg cacattaaaa aaatccctgc aattattttt cagttgcagg
     4441 gattttttat ttataagttt ttataatatt gcttttgatt cataattaaa acgttataaa
     4501 aaagccagaa agcttttatt tcttcttata cctgattttc tttaccccgt aaattatacc
     4561 gagtgccgct aaaaatccga ttccg
//
LOCUS       DAOPYB010000223         4584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_24171_length_4584_cov_7.280194, whole genome shotgun sequence.
ACCESSION   DAOPYB010000223 DAOPYB010000000
VERSION     DAOPYB010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24171_length_4584_cov_7.280194"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKH58_12035"
     CDS             <1..260
                     /locus_tag="PKH58_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89803.1"
                     /translation="KDVINEALNETLSRTFSTSMSVLVVLFAIFIFGGDTIRGFIFAL
                     LIGSILGVYSTLFVAVPIAYDIMLARTKKKELKAAKVELKK"
     gene            447..3311
                     /locus_tag="PKH58_12040"
     CDS             447..3311
                     /locus_tag="PKH58_12040"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNX89804.1"
                     /translation="MWKIRTLITASLISFQLFIFAQEFTPIVNQYSKKDYNASNQNWD
                     VKQAADGKMCFGNNQGLLQFDGSSWNVYKIPGNKIIRSLYISSDKKIYVGSFEEFGYF
                     EKDNKGMLVYSSISDKLRNYKMQNDEIWNILEYDGKIIFQSFTSFFTYDKRNVRGKRF
                     PLTFLFFQKFKNQIYTHTDQKGLCYYDKKTEDFVPINNTPFKSAVVAILPINHHEAFL
                     VTKADGIYIFNGIDFKRLVTDADRELAGANINKAVLTKDSLLVIGTIQNGVTALNYKG
                     QKVWTLNTNNVLQNNTVLGLYCDQENNLWLALDKGISMLRLGKSLNYIHSFSPSIGAI
                     YSVNFQEPDNLFIATNQGFYRAKLNIAAKRIENVTLDPRITGQVWDISRFDNQFFCGN
                     NEQTYEVDKTQSKVVSPVKGGICIKKGIIHGKEVLVQGTYTELCIYVKKNNVWTFSHS
                     VSNFLNPIQYIEIDYKGTIWAAHMHAGLYAIRLKEDLKTIESLQTYESLDNVHKVPIN
                     VFSINNRIVFTDNMAFYTYDDIRKKIIPYDLINNSLGRFAQAYRVCASSPNYYWFIRD
                     DEAALVNIQNDKISMIDHVQNSLFLNQTVDDYQNIFYLNDKISLITLENGLALHERQS
                     GKNNLSKNTLQISKVEVSSRKDRKIILFPVITDSEVKLSYKNNNVRFTVFYPVYSYLN
                     NLSFRYRLKGLDDVWNRTTPDNTKEYNYLSPGKYTFQVQALSNSNEILSTVSYDFLVE
                     PPFYWNTFSKILYILLFTLMVYFAYWIVKRNFRIKKQKIHEEQDAIRKKEIEKREQQI
                     IALKNEKLESEIVLKSKELAGSTMTIIKKNELMNTIKDEIVSLKKTLGTQYPNKYYDK
                     LIKLLDENTSSEEDWEIFQANFDRIHENFFRNLHLKYPELTSNDLRFCAYIRLNLSTK
                     DIAHLMNISPKGVEVGRYRIRKKIGIPSTKNLNEFLIEFK"
     gene            3510..>4584
                     /locus_tag="PKH58_12045"
     CDS             3510..>4584
                     /locus_tag="PKH58_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNX89805.1"
                     /translation="MKRLVTVIFSFSFCIWTFAQSNYVVPSTIKAENFPGHVELSWNN
                     SPGFTYKILRSFDVPSNFIEIAETNQGQYFDFLGKTEKVKTVYYRIIPKGLSLKNQKN
                     ISSEIVVERKKMRDEDLLDMTQRYTTRYFFDFAEPTTGMARERSNDVKGDIVTTGGTG
                     FGIMSLIAAIERKYITPAQGLETVGKIVSFLEKTERFHGAWAHWYDANTLKAFSFSKF
                     DDGGDMVETAFLVQGLLTAREYFKNGEATERILANRITQLWETVEWDWYTQGTDSLYW
                     HWSKNYGWKMNHRIKGFDETLITYILAASSPTYPIKASVYESCYKKSTYYFNGKSYYG
                     IKLDLGMEYGGPLFFTHYSFLGLN"
BASE COUNT         1531 a          743 c          885 g         1425 t
ORIGIN      
        1 aaaaagatgt gataaatgaa gccctgaatg aaacattaag ccgtactttt agtacctcga
       61 tgagtgtgtt ggtggtatta tttgcaattt ttatcttcgg aggtgatacc attcgtggat
      121 ttattttcgc tctgttaatt ggttctatcc tcggagttta ttccacatta tttgttgccg
      181 taccgattgc ttatgatatc atgttggcaa gaaccaagaa aaaggaactc aaagcagcaa
      241 aggttgaact gaagaaataa ttctcagcat aaatagatta gtaattcgta ttttttataa
      301 agctgaaaag ccctgtagtg attattactg cagggctttt ttatttaaaa aatatatata
      361 ttcaaaacac agtaatggta aaattatttt aactttgcat aacacgttaa gattaatttg
      421 cgttataatg taaaaaaaga atgaatatgt ggaaaattcg aactttgata acagcttctc
      481 tgatatcttt tcagctattt atcttcgcac aggaatttac acccattgta aatcaatatt
      541 caaagaaaga ttacaatgcc tcaaaccaga actgggatgt aaagcaagct gctgacggaa
      601 agatgtgttt cggtaataat cagggtttat tgcagtttga tggttccagc tggaatgttt
      661 ataaaatacc gggaaacaag attattcgtt cactgtatat ttcttctgat aagaaaatat
      721 atgtcggttc attcgaagaa ttcggatatt ttgagaagga taataaagga atgctggttt
      781 atagttcaat ctccgataaa cttaggaatt ataaaatgca gaatgatgag atatggaata
      841 tacttgaata tgacggaaag attatttttc agtctttcac gagttttttt acttatgata
      901 aacggaatgt aagagggaaa cgatttccgt tgacttttct ttttttccag aagtttaaaa
      961 atcaaattta tacccacaca gatcagaaag gattatgtta ttatgataaa aagacggaag
     1021 attttgttcc gataaataac actcccttta aaagtgcagt tgtagcaata ctcccgatta
     1081 atcatcatga agctttcttg gtgactaaag cagatggaat ctatattttt aacggtattg
     1141 atttcaaacg gttggtcacg gatgctgaca gggagttggc aggagcgaat ataaacaaag
     1201 ctgtattgac aaaggattct ttactggtca tcggaactat ccagaatggt gtgactgctc
     1261 tcaattacaa aggtcaaaag gtgtggacac ttaatacaaa caatgtgctg caaaacaata
     1321 ctgttctggg gttatactgt gatcaggaaa ataatctctg gttagctttg gataaaggca
     1381 tttccatgtt gcggcttgga aaatctttaa attatattca ttcattctca ccgtccatcg
     1441 gagccattta ttctgtaaat tttcaggagc ctgacaattt atttatagca actaatcagg
     1501 gattttacag ggcaaaattg aacatagcag ctaaaagaat agaaaatgtg actcttgacc
     1561 cccggattac cgggcaagtt tgggatattt ccagattcga taatcagttt ttctgcggaa
     1621 ataatgaaca gacttatgaa gtggataaaa ctcaaagtaa agtagtttca ccggtgaagg
     1681 gtggaatttg tatcaaaaaa gggattattc atggcaaaga agtgctggtg cagggtacat
     1741 acactgaatt atgtatttat gtgaaaaaaa ataatgtctg gacttttagt cattcggtta
     1801 gtaactttct caatcccatt cagtatattg aaattgatta taagggtact atctgggcag
     1861 cacatatgca tgcaggtctg tatgctatcc gcttgaagga agatctgaaa acgatagaga
     1921 gcctgcaaac ttacgaatcg cttgataacg tgcataaagt gcccattaat gtattttcca
     1981 ttaataaccg gattgtattt accgataata tggcttttta cacttacgac gacatccgga
     2041 agaaaattat tccgtacgat ttgattaata actccctcgg acgatttgcc caggcttatc
     2101 gggtttgcgc ttcgtcacca aattattact ggtttatccg tgatgatgaa gctgctcttg
     2161 tgaatataca aaatgataaa atcagcatga ttgatcacgt tcagaattct ttgtttctga
     2221 atcagacagt ggatgattat caaaatatct tttatttaaa tgacaaaata tcgttgatta
     2281 cactcgaaaa cggattggca ctgcatgaaa ggcaaagcgg aaaaaacaat ttgagtaaga
     2341 atacccttca gatttcaaaa gtagaagtga gtagccggaa agataggaag ataattcttt
     2401 ttcctgtcat aaccgactct gaagttaagc tatcctataa aaataacaac gtcagattta
     2461 ccgtgtttta tcctgtttat tcctacttga ataacctttc ttttagatac cgactgaaag
     2521 gattggatga tgtgtggaac cgaactactc cagataacac gaaagagtat aattatttgt
     2581 cgcctggaaa atatacattc caagtacagg cgctgtccaa cagcaatgaa atactttcca
     2641 ctgtttcgta tgattttttg gttgagcctc ctttctactg gaatacattt tcaaaaatac
     2701 tgtatatttt gctttttacc ctgatggttt attttgctta ctggatagtt aagcgcaatt
     2761 tcaggattaa aaaacagaaa attcatgaag aacaggacgc tatccgtaaa aaggagattg
     2821 aaaaaagaga acaacagatt atagctttaa aaaatgagaa attggaatcg gaaatcgttc
     2881 tgaagagtaa ggagcttgcc ggatccacca tgactattat aaaaaagaat gaactgatga
     2941 acacaatcaa agatgaaatt gtttcattaa aaaagacact gggaacacag tatccgaaca
     3001 aatattatga taaactgatt aaacttctgg atgaaaatac ttcttcggaa gaagattggg
     3061 aaatctttca agctaatttt gataggattc atgaaaattt ctttagaaac ttgcatttga
     3121 aatacccgga actgacttcg aacgacctcc ggttttgcgc atatatccgt ctgaatttat
     3181 ctaccaaaga cattgctcac ctgatgaata tttctccgaa aggggttgaa gtgggaagat
     3241 atagaatccg aaagaaaata ggtatcccat ccacgaagaa tctgaatgaa ttcctgatag
     3301 aattcaaata aatttctaaa aaatagtatt ctattaaact tcattgtttc aataatttat
     3361 gattaaattc tgcaagtatg tagtgaaaaa atagtattta tattctttgg atgtagtgtt
     3421 catgttgtgt cctaatattt tgaattagaa aaaacaattc ttttatttgc gtatcggtag
     3481 cccattaatt tttttagaac tgtgtgaaca tgaaaagatt agtaactgtt attttctcct
     3541 tcagtttttg catttggact tttgcccaaa gcaactatgt tgtgccatct accattaagg
     3601 ctgaaaattt tcccggtcat gtagaactca gctggaataa ttcccccgga tttacgtata
     3661 aaatcctgcg ttcatttgac gtaccctcaa attttattga aattgcagaa accaatcagg
     3721 gacaatactt tgatttttta ggtaaaacag aaaaagtcaa aactgtttat tatcgtatta
     3781 ttccaaaagg tctatcgctg aaaaatcaga agaatataag ttcagaaata gtcgttgagc
     3841 gaaaaaaaat gagggatgaa gatttactcg acatgactca gcgttacacc acacgttatt
     3901 ttttcgattt tgccgaaccg actaccggca tggcccgtga aaggagtaat gatgtgaaag
     3961 gagatattgt aactaccggt ggaaccggat ttggtattat gagtctcatt gctgccatcg
     4021 aaagaaaata catcaccccg gctcaaggat tggaaactgt tgggaaaatt gtcagttttc
     4081 tggaaaagac ggaacgattt catggcgcct gggctcactg gtatgatgca aatacgctga
     4141 aagcatttag ttttagcaaa ttcgatgacg gcggtgatat ggtggaaacc gcctttctgg
     4201 tacagggatt attaacagcc cgcgaatact tcaaaaacgg tgaagctaca gaaagaattc
     4261 ttgcaaaccg cattacccaa ctttgggaaa cggtagaatg ggactggtac acgcagggaa
     4321 cagattctct ttactggcac tggtctaaaa attacggatg gaaaatgaac catcgcatca
     4381 aaggatttga cgaaacgctg ataacctaca ttctggccgc ttcatctcct acatatccca
     4441 ttaaagcatc tgtatatgaa agttgctaca aaaaatccac atactatttt aatggtaaaa
     4501 gttattatgg aataaaactg gatctgggga tggaatatgg cggaccattg ttttttactc
     4561 attattcttt ccttggtttg aacc
//
LOCUS       DAOPYB010000224         4579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_24207_length_4579_cov_10.301503, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000224 DAOPYB010000000
VERSION     DAOPYB010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24207_length_4579_cov_10.301503"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKH58_12050"
     CDS             complement(<1..313)
                     /locus_tag="PKH58_12050"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNX89806.1"
                     /translation="MKIIVLGAGLVGGPMAIDLAKDNEFEVSCTDINPTSFGYLQTFG
                     INTLQHDLSDTETVKKLVSDFDMIINAVPGFMGFQTLKAIIEAGKNVVDISFFPEDPF
                     LL"
     gene            571..2028
                     /locus_tag="PKH58_12055"
     CDS             571..2028
                     /locus_tag="PKH58_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNX89807.1"
                     /translation="MKKVLLLCSMFLSLNLFSQGYWTQKADFGGTPKYWAVGFAVNGK
                     GYAGLGRGNGFSNSSDLWEYNPVTNAWTQKSSFPGGARYSSHVFVINNKAYFCSGAYW
                     SGISGDYTPYNDVWEYNPATDTWTQKNNFPGPGRHDAFAFSFGGMGYLCFGQDSNQLR
                     LNDLWEYNPNTDSWTQKATFPGTARSGGIQFGFEKYGYIGLGRDNNNVSLGDIWRYDC
                     STNTWSQMANFPSTSRTSASTFVLNRRAYVVCGYNFQTSSYSSQLWEYNPNTDTWMQR
                     ENFPAAARACGTAFSINNRGYFGLGNVNSTFKKDFWEYTPWCLDSINTDVFLTGNTIY
                     ANASGAVYQWLNCDSAFAQVSNATGQSFTPAWAGNYAVVISQDVCVDTSECIGVFCFD
                     TINTAVTLSGVSLYSLASGASYQWLNCDIGFAVIDNETNQSYSPPVNGNYAVVVTQGD
                     CSDTSACITFSAYGIDETENSGILIYANLLQKERL"
     gene            3022..4326
                     /locus_tag="PKH58_12060"
     CDS             3022..4326
                     /locus_tag="PKH58_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX89808.1"
                     /translation="MERYAMDVLKSWKNKQNRKPLIIRGARQVGKTWLMKEFGRMEYV
                     QTAYVNFESSSSLKMLFAENFDIPRIITALQIETGIQINPNNTLIIFDEIQEAEGALT
                     SLKYFCENAPEYNIIAAGSLLGVSLHKHTSFPVGKVEFLDLFPLSFTEFLLAFKQKNL
                     VDLLNSRDWNLIKSFKEKYIQHLRHYYYIGGMPEAVSSFSRNNDFVEVRAIQKRILSA
                     YEQDFSKHAPNETVPRIRMLWNSILAQLAKENRKFIYGALKTGSRAKDYELALSWLID
                     CGLIHKVSRVSKPGIPLKAYEDYSAFKLFAVDVGLLGAMGNIDVKTLLEGNAVFEEYR
                     GAMTEQYVLQQLILNDDMFIGYWSAEKSTAEIDFLIQYAGGVFPVEVKASENLQAKSL
                     KAFCMKYKNISAVRISMSDYRKEDWLTNIPLYGISEFTKEFE"
BASE COUNT         1394 a          797 c          943 g         1445 t
ORIGIN      
        1 tcagcaaaaa cgggtcttcg ggaaagaaag aaatatccac cacatttttc ccggcttcga
       61 tgatggcctt gagcgtttga aatcccataa aaccggggac tgcattgatg atcatgtcaa
      121 aatcagaaac aagttttttt actgtttccg tatccgataa gtcgtgttgc aaggtgttaa
      181 ttccaaaggt ttgcagatat ccaaaagaag tcgggtttat atccgtacaa ctgacttcaa
      241 attcattatc ttttgcaagg tcaattgcca ttggcccgcc tacaaggccg gcgccgagaa
      301 caataatttt catgtttgta tgttttattt tgcaaatcta cataaaaagc tgcatactaa
      361 ctataaccgg aaaccttccc gattgaaatt gttcccgaga ggtttgcaaa gaaaaaaccg
      421 cactgtagat tttcccttgg ggcaaagaac actgaaaacc tttgcacatt ttgcattgcg
      481 atattttgtg gccttatcgg caaaaaaatt attaaaactc catttctgat agtaatttta
      541 tttttaattt tgtaaaaaaa tatattatat atgaaaaagg tattgttatt atgttccatg
      601 tttttgtcct taaacctttt ttcacaggga tattggacgc aaaaagctga ttttggcggt
      661 actccaaaat attgggcagt tggttttgcc gtcaacggta agggttatgc aggcctgggg
      721 cgtggaaatg gtttcagtaa ttccagtgat ttgtgggaat ataaccccgt tacgaatgcc
      781 tggacccaga aatctagttt tccgggtgga gcaagatact catcccatgt atttgtcatt
      841 aataataaag catatttttg ttctggtgct tactggagtg gtatttctgg tgattatact
      901 ccttataatg atgtatggga atacaaccct gcaacagata catggactca gaaaaataat
      961 ttcccgggtc cgggcaggca tgatgctttt gccttttctt ttggaggtat ggggtatttg
     1021 tgtttcggac aggatagtaa tcaacttcgt ttaaatgatc tttgggaata taatcctaat
     1081 acggattcat ggacacagaa agctactttc ccaggtactg cacgatcagg aggaattcaa
     1141 ttcggatttg aaaaatatgg ttatataggt ttgggaagag ataataataa tgtttcttta
     1201 ggagatatct ggcgatatga ttgttccacg aacacatggt ctcaaatggc taatttccca
     1261 agtaccagcc gaacatcagc ttctacattt gttttgaatc gaagggctta tgtggtttgt
     1321 ggatataatt ttcagacttc atcttattct tctcaattat gggaatataa tcccaatacg
     1381 gatacctgga tgcaaaggga aaatttcccg gcggcggcaa gagcttgcgg aactgctttc
     1441 tctattaata acagaggcta ttttggatta ggtaatgtta atagtacgtt caaaaaagac
     1501 ttttgggaat atacgccctg gtgccttgat tctataaata ctgatgtatt tttaacggga
     1561 aacactattt atgccaatgc ttcgggtgcg gtgtatcaat ggcttaattg tgacagcgcc
     1621 tttgctcagg tgtctaatgc aaccggccag agcttcacac cagcctgggc tggaaattat
     1681 gcggtggtga tttctcagga tgtttgtgtt gatacatcgg aatgtatagg tgtcttttgt
     1741 tttgatacca taaataccgc agttacacta tcgggagtat ccttatactc gcttgcttcc
     1801 ggtgcctctt accaatggct taattgcgat ataggttttg ctgtgattga taatgagacc
     1861 aatcagagtt attcgccgcc tgtcaatgga aattacgctg ttgttgtaac tcagggtgac
     1921 tgtagcgata cctcggcatg tataacattc agcgcttatg gtattgatga aacagagaat
     1981 tccggcattt tgatttacgc aaacctgttg caaaaagaaa ggctatgata tggaatagtg
     2041 tgtgtaagca tattttaagt agcaaataat caatttatta agaaaataca aaaagattta
     2101 cccacgaata taacattaga tttatcaata attagaaaag caactacatt aatccatatc
     2161 atagccaaaa gaaataatca ttgatataaa aggaggcgag gtgaagtcgg atgtcgtgct
     2221 cacagatatg cttggaagga ttgtcaggac atatcatgtg aacagttcgg gcctgcaaaa
     2281 attcagtatg gggtccattc cggagggatt tattttgtta gctttgaaac caattatggg
     2341 aaagtcacaa aaaagttctt cgttacagaa tgatgcccgt tgtgcgaact ttgctcgttg
     2401 tgcgaagttt gcaacttcgc acactcctta attcaccatt ttcaactttg cattttgcag
     2461 caataactgt tttcgaccgc tgtgcgaagt ttgtaacttc gcacactcct caattttata
     2521 aaaaaattgt caatatgtcg attacaactc gacaaatttg tgtatatatg tcgaaaacaa
     2581 ttcgacatga tattgatttt aataaagtag cccgtgtttc atccgaaata ataccaattc
     2641 ttaatataaa ttagtccaga ggctaattta tattatagaa tggtaaatgc cgatcgtaaa
     2701 tatgtttagc cccgcctttg gcgggactat tacatattga gtatcggtat aaaactaaaa
     2761 gaatcatatt tgctaacacg gcagtttaac gaacacaaaa tgtgcatccc ttttgaaatg
     2821 ctttgaaagc tctttgtgcg aagtttgaaa atcgcacccc cgtggtgcga ttttgtaaat
     2881 cgcactccta agttgcaaaa aaaatttatt tatatttaaa agtcgtttga atttatgtaa
     2941 ctttgttcaa aaagacgttt gaatttatgt gattttgttc aaaaagacgt ttgaatttat
     3001 gtaatttaaa acaatttgtc tatggaaaga tacgcaatgg acgtgcttaa aagctggaaa
     3061 aacaagcaaa atagaaaacc tcttatcata cgcggtgctc gtcaggtagg aaaaacatgg
     3121 ctgatgaaag agtttggaag aatggaatat gtccaaactg cttatgtgaa ttttgagagt
     3181 tcaagctctt taaaaatgtt gtttgccgaa aatttcgata ttccaagaat tattactgcc
     3241 ctgcagattg agaccggcat acaaataaac cctaataata cactgattat ttttgatgaa
     3301 atacaagaag cagaaggagc gttaacctca ttaaaatatt tttgcgaaaa tgcgcctgaa
     3361 tataatatca ttgcagccgg ctctctgcta ggggtgtcgc ttcataagca cacttcgttc
     3421 ccggttggta aagtagaatt tttagacctt tttccgctta gttttactga gtttttgctg
     3481 gcgtttaagc aaaaaaacct ggttgatttg ttaaatagca gagattggaa tctgataaaa
     3541 agttttaaag aaaaatacat tcagcactta cgacactact attatattgg cggaatgccc
     3601 gaagcagtgt cttctttcag caggaataac gatttcgtgg aggttcgtgc tattcagaaa
     3661 cgaatattgt ctgcttacga acaggatttt tcaaaacatg cccccaacga aactgtacca
     3721 aggataagaa tgttgtggaa ttctatatta gcgcaacttg caaaagaaaa tcgtaaattt
     3781 atttacggcg ctttaaaaac aggctcaaga gccaaagatt atgagcttgc cttatcatgg
     3841 cttattgatt gcggattgat acataaagtt agtcgggtat caaaaccggg aattccttta
     3901 aaagcatacg aagattacag cgcatttaag ttatttgctg tggatgttgg tttactggga
     3961 gctatgggga atattgacgt gaaaaccttg cttgaaggta atgctgtttt cgaggagtat
     4021 agaggcgcaa tgacagaaca atatgttttg caacaactga ttttaaatga tgacatgttt
     4081 attggctact ggtcggcaga gaaatctact gctgaaattg attttctgat acaatatgca
     4141 ggtggtgtat ttccggttga agttaaggca tctgaaaact tgcaggcaaa aagccttaaa
     4201 gcattttgca tgaaatataa gaatatatca gcagtcagga tttctatgtc tgattacagg
     4261 aaggaagact ggctgacaaa tatacctctt tatggtatta gtgagtttac taaagagttt
     4321 gagtgaaaga aataaattct tttatattcg tagcacggtt caaagcccta ctgcgaattc
     4381 attaatttat tccgttgtgc gattttgcaa atcgcactcc ttaattcaca atcttcagct
     4441 tcgcatactg cagaagcaac tgtttttcgc ctacaccttc aaacaatacc gtggtttttt
     4501 tgtttggccc gttgccttcg atgctcatca cgcggccccg gccgaaacgc tggtgttcca
     4561 cggtcattcc gacacggat
//
LOCUS       DAOPYB010000225         4538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_24536_length_4538_cov_9.889137, whole genome shotgun sequence.
ACCESSION   DAOPYB010000225 DAOPYB010000000
VERSION     DAOPYB010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24536_length_4538_cov_9.889137"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..3839
                     /locus_tag="PKH58_12065"
     CDS             6..3839
                     /locus_tag="PKH58_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX89809.1"
                     /translation="MNVAVKASFTLFLLFGLSGSMRAQLKADASAPGQTRIEQAVKLN
                     PTTVEIKFTNQQKMLIDFYGNNIFRMFQDNSGKGLRDPQADPQAQILVNNPRKPLAGL
                     NVTDNNNTVTLATSAVQLTFDKNSTLFTIRNLKTNQMVVKSIQPVAFEKNKFTIALSE
                     EADEYFYGGGVQNGRFSHKGKAIAIENQNSWTDGGVASPTPFYWSTKGYGFMAYTFKK
                     GKYDFGAKEKNTVKLSHDEDYLDVFFMVNDGVVALLNDFYQLTGNPQLIPKFGFYEGH
                     LNAYNRDFWKETDKGGVLFEDGKRYQEAQKENGGIKESLNGEKNNYQFSARAVIDRYK
                     QHDMPLGWILPNDGYGAGYGQTSTLEGNIRNLKEFGDYARKNGVQIGLWTQSDLHPKE
                     GVEALLQRDIKNEMIVAGVNVLKTDVAWVGAGYSFGLNGIQDVANIMTKYGITARPFI
                     ISLDGWAGTQHYATIWSGDQTGGVWEYIRFHIPTYIGSGLSGQPNITSDMDGIFGGKN
                     MPVNIRDFQWKTFTPMQLNMDGWGANEKYPHALGEPAASINRNYLKLKSELLPYTYSI
                     AREAVNGLPIIRAMFLEEKNSYTLGKQTQYQFMYGPNFLVAPVYQNTKSDTLGNDVRN
                     HIYLPKGSWVDYFTGQVYEGGRVINNFDCPIWKLPIFVKKGAIIPMANPNNNPDEVNN
                     NLRIYEVYPSGNTCFKQYDDDGKSTAYLQGKSTETVIKSDVNGSKATIAVNATTGSFD
                     GFVKEKATEFKVNVTKQPKKVTVRVGGKKVGLKVAKSQEDYNKRENVYFYNAAPEFNR
                     FSTKGSEFENVKITRNPQVWVKVAQTDITKNVVSLEMKGFEFNPVNTLLQKSGTLSPL
                     RNAGVTDNNTAAYKLTPSWDKQPNADYYEIELDGMVYSTIKDNELIFENLTPETEYAF
                     KARSVNKDGASAWTGFTAKTTADPLQFAVKGITAESTALMQPGQGAVRLFDYDEGNMM
                     HSEYKTNAIPCDFILDLKSVNHLDKMVYLPRTSGLNGMIQQANVYYSEDKVNWTEAGS
                     IDWKTTAEDKTFDFTFHPVARYIKINVTKAVGNFVSGRELYVFKVPGTESFLPGDINN
                     DRVVDSNDYTSYMNYTGLRLGDGDFEGYVSKGDVNKNNLIDAYDISNVATLLEGGISQ
                     SAIAKLTGKLALTASKASYNAGDTVAITVQGADLKSVNALSFAIPYNKNEYEYVGIDQ
                     LNVKWMQNLTYDRLHTNGDKVVYPTFVNLGNETPLEGNETLFVLKFKAKRAVKFNLTQ
                     KQGLLVDKFLNTISF"
     gene            complement(3973..4323)
                     /locus_tag="PKH58_12070"
     CDS             complement(3973..4323)
                     /locus_tag="PKH58_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNX89810.1"
                     /translation="MNVEDIRNYCLSLKGAEECFPFDDETLVFKALGKMFAFIPLEES
                     ELFINLKCDPVKALNLREKYLAVQPGYHMNKKYWNTVALTNEVTEKLLKEWIKHSYHE
                     VMKGIPKKKRECYV"
     gene            complement(4443..>4538)
                     /locus_tag="PKH58_12075"
     CDS             complement(4443..>4538)
                     /locus_tag="PKH58_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNX89811.1"
                     /translation="AYYLKYQSKRAAYVKAFWNVVNWNKVSELYR"
BASE COUNT         1387 a         1031 c         1016 g         1104 t
ORIGIN      
        1 taattatgaa tgttgctgtt aaggcttcat tcaccttatt cctgttgttc ggtctgtccg
       61 gcagcatgcg tgctcagcta aaagccgatg catctgcacc gggtcaaacc cgcatagagc
      121 aggcggtaaa gctcaatccg acaacagtcg aaatcaaatt caccaatcag caaaagatgc
      181 tgattgattt ctacggaaac aacattttcc gcatgtttca ggacaactcc ggcaaaggat
      241 tgagagaccc gcaggctgac cctcaggctc agattctggt caataatccc agaaaaccgt
      301 tagccggact gaacgtgaca gacaacaaca ataccgttac gctggcaacc agcgctgttc
      361 agctgacttt tgataaaaat tccacacttt ttaccatccg aaatctgaaa accaatcaaa
      421 tggtagtaaa atcaatacag ccggtggctt tcgaaaaaaa taaatttacc atcgcgctca
      481 gcgaagaggc cgatgagtat ttttatggtg gcggcgtgca gaacgggcga ttctcacaca
      541 agggaaaagc catcgccatc gagaaccaga acagctggac cgatggaggt gtggcttcac
      601 caactccgtt ttactggtca accaaaggct atggttttat ggcatatact ttcaaaaaag
      661 gaaagtatga ttttggagct aaagaaaaaa acaccgtgaa actctcgcac gatgaagatt
      721 acctggatgt tttctttatg gtaaatgatg gtgtcgttgc tttacttaac gacttttacc
      781 agctgacagg aaatccacag ctgattccta aattcggttt ctacgaaggt catctcaacg
      841 cttacaaccg tgacttctgg aaagaaaccg acaaaggcgg cgtacttttc gaagacggca
      901 aacgctatca ggaagcacaa aaagaaaacg gtggtatcaa agaatctctc aacggagaga
      961 aaaataacta ccagttctct gcccgggcag taattgaccg ttacaaacaa catgacatgc
     1021 cgctgggctg gattttaccc aacgatggct acggagccgg ctacggacaa acctctacac
     1081 tggaagggaa catccgaaac ctgaaagaat tcggagatta tgcccgcaaa aatggcgtac
     1141 agatcggatt gtggacacag tctgacctgc atccgaaaga aggcgtggaa gcgctgctgc
     1201 aaagagatat taaaaatgaa atgattgttg ccggtgtaaa tgtgctcaaa acagatgttg
     1261 cctgggtggg ggccggttat tcattcggac tgaacggaat acaggatgtc gcgaatatca
     1321 tgacaaagta cggtattact gcacgtccgt tcatcatttc actcgacgga tgggccggaa
     1381 cccagcatta cgcaaccatc tggtcgggcg accagaccgg aggcgtatgg gaatatatcc
     1441 gtttccacat ccctacatac attggttcag gcctttcggg gcaaccaaac attacttcgg
     1501 atatggacgg aattttcggg ggaaaaaata tgccggtcaa tatccgcgac ttccagtgga
     1561 aaacattcac tcccatgcag ctgaacatgg atggctgggg tgccaatgaa aaatacccgc
     1621 acgcgctggg cgaaccggct gcgtccatta accgcaatta cctgaagctg aaatcagaac
     1681 ttctgcctta tacctacagc atagcccgcg aggcggtaaa tggtctgccc atcattcgcg
     1741 ccatgtttct ggaagaaaaa aattcctata ccctgggtaa gcaaacccaa taccagttta
     1801 tgtacggacc gaatttcctg gtcgcgcctg tttaccagaa taccaaatcg gatacgctgg
     1861 gcaacgatgt gcgtaatcat atttacctgc ccaagggaag ctgggtggat tatttcaccg
     1921 gacaggttta tgaaggcggt cgtgtgatta ataatttcga ttgccccatc tggaaactgc
     1981 ccatatttgt gaagaaaggt gcgattatcc caatggctaa tccgaacaat aacccggacg
     2041 aggtaaacaa caacctgaga atttacgaag tatatccgtc aggaaatacc tgttttaaac
     2101 aatacgacga cgatggaaaa tctacagcct atttgcaggg taaatctacc gaaactgtta
     2161 ttaaatcgga tgtgaacggc agcaaagcaa ccatcgctgt gaatgccact accggtagtt
     2221 tcgatggttt cgtaaaagaa aaagctaccg aatttaaagt aaatgtgact aaacagccaa
     2281 aaaaagtaac tgtcagagtg ggtggcaaaa aagtaggtct gaaagttgcc aaatctcagg
     2341 aagattacaa caaacgcgag aatgtttact tctacaatgc agctcctgaa ttcaaccggt
     2401 tctccaccaa aggcagtgaa tttgaaaacg taaaaatcac cagaaatccg caagtctggg
     2461 tgaaagtggc tcagaccgac ataaccaaaa atgttgtgtc tctcgaaatg aaaggtttcg
     2521 aattcaatcc ggtcaataca ctgctgcaaa aatcaggcac gttgtcgccg ctgcgaaatg
     2581 ccggtgttac cgataacaac acggcagcct ataagctgac cccaagttgg gacaaacagc
     2641 caaacgccga ttattacgaa attgaactgg acgggatggt ctattccacc atcaaagaca
     2701 atgaattgat tttcgaaaac ctcacacctg aaaccgaata cgctttcaaa gcccgctcgg
     2761 taaataaaga cggtgcttca gcatggaccg gcttcacagc aaaaaccact gccgatccgt
     2821 tacagtttgc cgtgaaaggt attactgccg aaagtacagc attgatgcaa cccggtcaag
     2881 gtgccgttcg tctgttcgat tacgacgaag gtaatatgat gcactctgaa tacaaaacga
     2941 atgcaattcc ctgcgatttc atcctggacc tcaaatcggt taaccatctc gacaagatgg
     3001 tgtacctgcc acgtaccagc ggcctgaatg gtatgataca gcaagcaaac gtgtattaca
     3061 gcgaagataa ggtgaactgg acagaagccg gatcaattga ctggaaaacc actgccgaag
     3121 acaaaacttt cgacttcaca tttcacccgg ttgcacgcta tatcaaaatc aatgttacca
     3181 aagcggtggg caactttgtt tcgggacgcg aattgtatgt gttcaaggtg cctggaaccg
     3241 aaagcttcct tccgggtgat atcaacaacg accgtgtggt ggacagcaac gactatactt
     3301 catacatgaa ctataccggt ttgcgtctgg gcgatggtga cttcgaagga tatgtaagca
     3361 aaggtgacgt caataaaaac aacctgattg acgcttatga catttcgaac gtggctacac
     3421 tgctcgaagg cggcatcagc cagtcggcta ttgccaaact gaccggcaaa ctggccctga
     3481 cagcatcgaa agcaagttat aacgcaggcg acacagtggc cattacagtg cagggtgctg
     3541 acctgaaatc ggtcaacgcg ttgagcttcg cgatacctta caacaaaaat gaatacgaat
     3601 atgtgggtat cgaccagctg aatgtaaaat ggatgcaaaa cctcacttac gaccgtctgc
     3661 acaccaacgg ggataaagtc gtgtatccca catttgtgaa tctgggcaac gaaacgccac
     3721 ttgaaggcaa cgaaacgttg tttgtgctga agttcaaagc caaacgcgcc gtgaaattca
     3781 atctcactca gaaacaaggt ctgttggtag ataaattcct gaataccatc agtttttaat
     3841 ccgattgtcc aaataataaa aaatgctcag atcacagggt ctgagcattt ttattctgaa
     3901 aagtcaggag aaaataaatg cccgggttat tgaaaaccag agtatcattc tttttgtaag
     3961 tacatcatgt aatcatacat aacattctct tttctttttc ggtattcctt tcattacttc
     4021 gtggtaggag tgtttaatcc actctttgag tagtttctcg gtgacttcat tcgttagcgc
     4081 cacggtgttc cagtatttct tattcatatg atatccgggc tgaactgcca gatatttttc
     4141 ccgcaggttt agggctttca caggatcaca cttcaggttg atgaataatt cggactcttc
     4201 cagtggtata aaagcaaaca ttttaccgag ggctttaaat acaagtgtct catcgtcaaa
     4261 aggaaagcat tcttcagctc ctttcagaga caaacaataa tttcgaatat cttctacatt
     4321 cataaaccga taaattcttt gtgctgactg cataaagcag ccagtcaggt tggttgtttg
     4381 ttgacagaaa tcctgaaatt caggcttttt ctgaaataaa tcagaaagag tgtcgggatg
     4441 cgtcagcgat aaagctcact taccttattc cagttcacca cgttccagaa tgctttgaca
     4501 taagcggccc gtttcgactg gtatttgagg tagtaagc
//
LOCUS       DAOPYB010000226         4490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_24954_length_4490_cov_10.908230, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000226 DAOPYB010000000
VERSION     DAOPYB010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24954_length_4490_cov_10.908230"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1173
                     /gene="panC"
                     /locus_tag="PKH58_12080"
     CDS             325..1173
                     /gene="panC"
                     /locus_tag="PKH58_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNX89812.1"
                     /translation="MRIIKTINELNSLIKEIKSENKTIGFVPTMGALHNGHLTLVKQS
                     AAENDVCVVSVFVNPTQFNNKTDLEKYPRNLEKDAEMLETAGCACVFAPSADELYSAD
                     ELNNTFEFDFGGMDQVMEGKFRPGHFNGVVQIVSKLFSLVQPDKAYFGKKDFQQLAII
                     HRMVRLMNFKIDIIDCPIVREASGLAMSSRNERLSEAEREIAVNISRILNESRNLQPE
                     KTPAELISWVTDEVNAISGLEVEYFEIADPVTLTAIDAWLPQSIGCIAVYCGEVRLID
                     NIEYCL"
     gene            1195..1545
                     /locus_tag="PKH58_12085"
     CDS             1195..1545
                     /locus_tag="PKH58_12085"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNX89813.1"
                     /translation="MLLNILKSKIHRVSVTEANLNYIGSITIDEDLMDAANIIANEKV
                     SIVNNNNGERFETYVIPGERNSGIICLNGAAARLVQPGDVVIIMAFAMMEMDEARNFK
                     PAVIFPDTKTNKLI"
     gene            1564..2166
                     /gene="hisH"
                     /locus_tag="PKH58_12090"
     CDS             1564..2166
                     /gene="hisH"
                     /locus_tag="PKH58_12090"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNX89814.1"
                     /translation="MKFYHMNVAILKYNAGNIRSVLFALQRLGIEASVTDDHEELMSA
                     DKVIFPGVGEASSAMQYLQDKNLDKLIVQLKQPVLGICLGMQLMCSYSEENNTNCLGI
                     FEQQVRKFSDTDFKVPQIGWNNIYNLSSPLFTGINEKEYMYFVHGYYVEKCAQSIATT
                     DYTLEYSAALQKNNFFAVQFHPEKSGNAGQQILQNFLSII"
     gene            2181..3311
                     /gene="tgt"
                     /locus_tag="PKH58_12095"
     CDS             2181..3311
                     /gene="tgt"
                     /locus_tag="PKH58_12095"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNX89815.1"
                     /translation="MNFELQHKDPNSNARTGLITTSHGQIETPIFMPVGTVGSVKAVH
                     FHELKEDIKAQIILGNTYHLFLRPGIEILEQAGGLHKFNGWDKPILTDSGGFQVFSLS
                     GTRKMKEEGVTFHSHIDGSKHLFTPERVVDIQRIIGADIIMALDECTPGTADYQYARK
                     SMELTHRWLQRGIKQFDATEPKYGYSQTFFPIVQGCVYPDLRKQSAEFIASQNREGYA
                     IGGLAVGEPTEKMYEMIEVVNEILPADKPRYLMGVGTPINILEGIERGVDMFDCVMPT
                     RNGRNGMLFTSQGIMNMRNEKWKNDFSPLDEYGTSYVDRVYSKAYLRHLFISKELLAM
                     QIASIHNLAFYLWLVGEARKHIVTGDFSSWKKEMVLQLGKRL"
     gene            3345..4445
                     /locus_tag="PKH58_12100"
     CDS             3345..4445
                     /locus_tag="PKH58_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNX89816.1"
                     /translation="MNINYRKIGLKRIDTYIIKKFLGTFFFSIILILSIAVIFDLVEK
                     IDDFFDNNAPFKAIVLDYYMSFIPYYANLFTPLFTFISVIFFTSKIANNTEIVAILSS
                     GVSFIRLMRPYLVSALVITLFSFLLGAFVIPPATAKLLDFEDKYIEKFKSDNARNVQM
                     EVDKNVIMYIERYEISSNTGYRFSLEKFNKKTLVSRLTAESITWKSKNNWTVNNYIKR
                     DFNGMRETLTRGAVLDTVIKVQPKEFFITSKEAPQMNLLDLHSYLVTQKKRGIGNIQA
                     FEDEYFRRFSMPISVLIMTLIGVSLSSRKVRGGMGLNLGIGLALSGLYVLFISMSTSF
                     AVSGAMPTVLAVWLPNIVFLTVGLILYKKAPK"
BASE COUNT         1369 a          946 c          945 g         1230 t
ORIGIN      
        1 tttaccttcg gcttttccgt tgccaaaggt gtaaactctt tttgtgtcac tcatatttta
       61 taattattta aatgtatatt ttcaaaattg tcaatcagcg ctccgcgaca ggttattaca
      121 gcattcgtaa ggctcgaaac actaattatt tatgatgatt ccgcaaagat aaagttttta
      181 gttaacatat aaaaatatag tatcttaatt aatactccgt tgaataaaaa tttgtttctt
      241 ctaccggaat aatggtgctt aaaacaaatt tgttttatac ttttgcagtc tgaatttcaa
      301 cctgatataa ttattcgaaa aggaatgaga ataataaaga ctatcaatga attaaattca
      361 ctgataaagg aaattaaatc cgaaaataaa actattggtt ttgtgccaac catgggtgct
      421 ttgcataatg gtcatctgac tcttgtgaaa cagagtgccg ctgaaaacga tgtatgtgtg
      481 gtaagtgtat ttgtcaatcc gacacaattc aacaacaaaa ctgatcttga aaaatatccg
      541 cgcaacctgg aaaaagatgc cgaaatgctc gaaaccgcag gatgtgcctg tgtttttgcg
      601 ccatcagcgg atgaattata ctctgctgac gaactgaata atacctttga attcgatttt
      661 ggaggtatgg accaggtgat ggagggtaaa tttcgccccg gacactttaa cggtgtggta
      721 cagattgtta gcaaattatt cagtctggtg cagcccgaca aagcctattt cgggaagaaa
      781 gactttcagc aactggccat catccaccgc atggtacgac tgatgaattt caaaattgat
      841 attatcgatt gccccattgt acgcgaagca agcggacttg ccatgagcag ccggaatgaa
      901 agattgtccg aagcagaacg cgaaattgca gtaaacattt caagaattct gaacgaaagc
      961 cgtaacctgc agcctgaaaa aacaccagcc gaactaatca gctgggtaac cgacgaggta
     1021 aatgccattt ctggtctcga agtggaatat ttcgaaatcg ctgatccggt cacactcaca
     1081 gctatcgatg catggctgcc gcaaagcatc ggctgcattg cagtgtactg tggggaagta
     1141 cggctcattg acaatataga atactgttta taatactaaa ccgaaaaaat catcatgtta
     1201 ctcaacattc tgaaatcgaa aattcaccgg gtatcggtca ccgaagccaa cctgaattat
     1261 ataggaagca taaccatcga tgaagatctg atggatgccg ccaatatcat agctaacgaa
     1321 aaagtcagca tcgtaaacaa caacaatggt gaacggtttg aaacatatgt cattcccggc
     1381 gaacgcaatt cgggaattat ttgtctcaac ggcgctgctg cccggctggt acagcccggt
     1441 gatgtggtta ttatcatggc attcgcgatg atggaaatgg atgaagccag aaatttcaaa
     1501 cccgcggtca tttttccgga taccaaaacc aataagttga tttaacacct gatcattccc
     1561 aatattaaat tttatcacat gaatgttgcc attctgaaat acaatgccgg aaatatccga
     1621 tcggtgcttt ttgcgcttca gcgtcttggc atcgaagcca gcgtaacaga tgaccatgaa
     1681 gaattaatgt cggctgacaa agtcattttt ccgggtgtgg gcgaggcttc ttctgccatg
     1741 cagtatttgc aggataaaaa tctggacaaa cttatcgttc agctgaaaca acctgtactt
     1801 ggcatctgcc tgggaatgca actgatgtgc agctattctg aggaaaacaa tacaaactgc
     1861 ctgggtattt tcgaacagca ggtacgaaaa ttttcagaca cagacttcaa ggttccgcaa
     1921 attggctgga ataatatcta caatttgtcg tcacccctgt ttacgggcat caatgaaaaa
     1981 gaatatatgt attttgtcca cggttattac gtggaaaagt gcgctcaaag tatagccaca
     2041 accgattaca cgctggaata cagcgcagcc ctgcaaaaaa ataatttctt tgccgttcag
     2101 tttcatcccg aaaaatcggg gaatgccgga cagcagattt tgcagaattt tttaagtata
     2161 atctgatttt gaaaaattaa atgaatttcg aactacaaca caaagacccg aacagcaacg
     2221 cacgtacagg acttatcacc accagccacg gtcagattga aacgcccatt ttcatgcccg
     2281 taggcactgt cggatcggtt aaagcagttc attttcatga attaaaggaa gatatcaaag
     2341 cccagatcat actgggtaac acctatcatt tatttcttcg tcccggaatc gaaatcctcg
     2401 aacaggccgg cggacttcat aagttcaacg gatgggacaa gcctattctc accgacagcg
     2461 gaggatttca ggtattttct ctttcgggaa ccaggaagat gaaagaagaa ggggttacat
     2521 tccactcaca catcgacgga agcaagcacc ttttcacacc cgaaagagta gtcgatattc
     2581 agcggataat cggagccgac atcatcatgg cgctggacga atgcacaccc ggcactgccg
     2641 attatcagta tgccagaaaa tcgatggaac tgacacaccg ctggcttcag cgaggtatta
     2701 aacaattcga tgccactgaa cccaaatacg gttattcaca aacatttttc ccgatagtac
     2761 agggttgcgt gtatcccgac ctgagaaagc agtcggctga atttatcgct tcacaaaacc
     2821 gcgaaggata tgccatcgga ggacttgccg taggtgaacc taccgaaaaa atgtacgaaa
     2881 tgatcgaggt agtcaatgaa attctgccag ccgataaacc gcgttacctg atgggcgtgg
     2941 gaacaccgat caatattctt gaaggaattg agcgtggcgt ggatatgttt gattgcgtaa
     3001 tgcctacccg caacggacgc aatggaatgc ttttcacctc gcagggcatc atgaatatgc
     3061 gcaacgaaaa atggaaaaat gatttctcac ctctcgacga atacggaact tcgtatgttg
     3121 acagggtata ttcgaaggct tatcttcgtc acctgtttat cagtaaagag ctgctggcta
     3181 tgcagatagc ttccattcac aatcttgcat tttatctctg gctcgtaggc gaagcccgca
     3241 aacacatcgt aaccggtgat ttttcatcgt ggaaaaaaga aatggtactg caactgggca
     3301 aacggttgta aaaaatatat tctgaaactt acagaagtaa aaacatgaac ataaattatc
     3361 ggaaaatcgg cctcaaaagg attgacacct atattatcaa aaagtttctg ggaacttttt
     3421 tcttttcaat tatcctcata ctgagtatag ctgtcatctt cgacctggtg gagaaaattg
     3481 atgatttttt cgataacaat gctccattca aagctattgt actggattat tacatgagtt
     3541 ttatccccta ctatgccaat ctgttcacgc cgctgttcac ctttatttcg gtaatctttt
     3601 ttacttccaa aattgcgaat aatacagaga tagtcgccat cctgtcgagc ggagtaagtt
     3661 ttatccgact gatgaggcct tatctggttt ccgcgttggt tatcacatta ttctcctttc
     3721 ttctgggagc atttgtcatt cctccggcta cagccaaact gcttgatttc gaggataaat
     3781 atattgagaa attcaagtcg gacaacgcgc gcaatgttca gatggaggtg gacaagaatg
     3841 taatcatgta catcgaacga tacgagatat cgtcaaatac cggataccgc ttctcactcg
     3901 aaaagttcaa taagaaaacc cttgtttcgc gtctaacggc cgaatccatt acctggaaat
     3961 cgaaaaataa ctggactgtg aataattaca taaaacgtga tttcaatggg atgagagaaa
     4021 cgctgaccag aggagcggta ctggatacag ttatcaaggt acaacccaaa gaatttttta
     4081 tcacctccaa agaagcgcct cagatgaact tgctggattt acacagctat ctggttaccc
     4141 agaaaaaaag aggtattgga aatatccagg cgtttgagga tgaatatttc cgccgttttt
     4201 ccatgcctat ttccgtactt atcatgacat taatcggcgt atcgctttcg tctcggaaag
     4261 tgagaggcgg catgggactg aacctcggta tcgggcttgc attgagcgga ttgtacgtgc
     4321 ttttcatatc catgtccacc tcattcgcgg tgagcggagc aatgccaact gtactggctg
     4381 tatggctccc aaatatcgtt ttcctcacag tgggattaat cctttacaaa aaagcgccga
     4441 agtagattca cgttttgtgt ttcacgtttg acgtttatag tttatagttt
//
LOCUS       DAOPYB010000227         4487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_24979_length_4487_cov_9.281814, whole genome shotgun sequence.
ACCESSION   DAOPYB010000227 DAOPYB010000000
VERSION     DAOPYB010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24979_length_4487_cov_9.281814"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1986)
                     /locus_tag="PKH58_12105"
     CDS             complement(7..1986)
                     /locus_tag="PKH58_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNX89817.1"
                     /translation="MIFDLSSKITLERIPEKYFRSTDPLEATRQSRYEKLRTEIFETE
                     KEGALAVAKEISALIRAKEEQNKPCVLAIPGGSSPLKVYADLVQMHKKEGLSFKNVYI
                     FNISDFYPVDNNHHSNARLINEYLISKVDLPADHFFTPSNIERSDVINFCHRYEAKME
                     ELGGLDFVLATIGQLGNLAYNEPGSQTGSVTRLMLLDAQSRSDLQQFYISSEEVPVSA
                     ITLGISTILSAKKVVLIAWGETKSNKIKEIIEGKANDSVPASFLQLHKNAHAVLDLTA
                     AQHLTRISHPWLVGPCEWTDKLIRRAIVWLCEKTQKPILKLTNKDYNQYGLNELLALF
                     GSAYNVNIKIFNDLQRTITGWPGGKPNADDSNRPERANPYPKRVLLFSPHPDDDVISM
                     GGTFQRLVEQKHIVHVAYETSGNIAVGDEEVVRYLSLMSNILERFDADNHALKTRYED
                     LRHLLTKEKKSGDVDNADILYLKGRIRREEARTACHYNGVPLERVHFLDLPFYETGKI
                     KKGDLTEKDVQIVMDLLREVKPHQIFVAGDLADPHGTHRVCLNAALAAIHELKNEEWM
                     KECRVWMYRGAWAEWEIDHIEMAVPISPEELRRKRNSILKHQSQMESAPFLGNDERLF
                     WQRAEERNQATAKMYSNLGLASYEAIEAFVQYTIL"
     gene            complement(2021..2467)
                     /locus_tag="PKH58_12110"
     CDS             complement(2021..2467)
                     /locus_tag="PKH58_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNX89818.1"
                     /translation="MIYSTLSESKRIEGLHPKFKQLFDYVKEHDFLGQEIGRITLDDD
                     NLFINNVNPQLLPADEQVLEVHRRYLDVHIPLDKAEIIGIKALEHCSTLKSEYNSQDD
                     CALYAGKPDNYITVQPGEFLIVYPEDAHAPIIGEGKIRKLVVKVMI"
     gene            complement(2783..3076)
                     /locus_tag="PKH58_12115"
     CDS             complement(2783..3076)
                     /locus_tag="PKH58_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89819.1"
                     /translation="MRIHLVIFVLLFSFTFGAYQEAESKPVRVYLTEFYPVPAEFGNI
                     SKGNFASFAGLIDDNLIVAGGCNFPDVPAADGGKKIFYKDILQFYGKKWEKTG"
     gene            complement(3548..3793)
                     /locus_tag="PKH58_12120"
     CDS             complement(3548..3793)
                     /locus_tag="PKH58_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNX89820.1"
                     /translation="MNIYVGNLSYKVRENDLKEVMEEYGQVDSCKLIIDRDTRRSKGF
                     AFVEMADDDAAKKAIAELNGAELDGRTMVVKEATPRN"
BASE COUNT         1327 a          889 c          815 g         1456 t
ORIGIN      
        1 tatttcttat aagatagtgt attgtacaaa ggcttcaata gcttcataag aagcgagtcc
       61 taaattgctg tacatttttg ctgttgcctg atttcgttct tcagcccgtt gccagaataa
      121 tcgttcgtca ttaccgagga aaggcgcgct ttccatttgc gactggtgtt tgaggatcga
      181 gtttctcttg cggcgaagct cttccggact aatcggtaca gccatctcga tgtggtcaat
      241 ttcccattct gcccatgctc cccgatacat ccacacacgg cattctttca tccactcttc
      301 gtttttcaat tcgtgaatgg cagccagagc agcgttcaga caaacacggt gtgtaccatg
      361 tgggtcagca agatcaccgg caacgaatat ctgatgtggt ttcacttccc gaagcaaatc
      421 cattactatc tgaacatctt tttcggtcag gtcacctttc ttgatttttc ctgtttcgta
      481 gaatggcaga tcgagaaaat gaacccgttc gaggggaact ccattgtaat gacaggcggt
      541 gcgcgcttct tcccgccgga tgcgtccctt caggtacagg atatcggcat tatcaacatc
      601 acccgatttc ttttctttgg taagcaaatg acgtaaatcc tcatatcttg ttttcagagc
      661 atggttatcc gcgtcaaaac gttcaaggat attactcatc aatgataggt atcgaactac
      721 ttcttcatca cctacggcaa tgtttcccga agtctcataa gctacatgaa cgatgtgttt
      781 ttgttcaact aagcgctgaa aggtaccacc catcgaaatt acatcatcat cgggatgggg
      841 actgaataaa agcacacgct taggatatgg atttgcacgt tccggacggt tgctatcatc
      901 cgcatttggt tttccacccg gccaaccggt tattgtgcgt tgcaaatcat tgaatatttt
      961 aatatttaca ttgtatgcag aaccaaataa tgcgagcagt tcatttaatc cgtactggtt
     1021 gtaatctttg ttggtgagct tcagtatagg tttctgcgtt ttttcacaca accagacaat
     1081 tgctctgcgg ataagtttat ctgtccattc gcagggacct accaaccagg gatgactgat
     1141 acgggtcagg tgctgagctg cagtcaggtc aagaactgcg tgtgcatttt tatgtagttg
     1201 cagaaacgat gcaggaacag aatcatttgc cttaccttca attatttcct tgattttatt
     1261 cgattttgtt tcaccccagg ctattaaaac aacttttttt gcagataata ttgtagaaat
     1321 tcccaatgtt atcgcgctta caggtacttc ttcagacgaa atgtaaaact gttgcaaatc
     1381 gcttctggac tgagcatcga gcaacatcag acgtgtaacc gaacctgttt gtgacccggg
     1441 ttcattatat gcaagattgc ctaactgacc gatagttgca agtacaaaat ccaaaccgcc
     1501 cagttcttcc attttggctt cgtaacgatg gcagaaattt attacatcag atctttcgat
     1561 gttggatgga gtgaaaaaat gatctgcagg taaatcaacc ttacttatca aatactcatt
     1621 aatcaaccgt gcattagaat ggtgattatt atccacggga taaaaatcgg agatattgaa
     1681 aatataaacg tttttgaagg aaagtccctc ttttttgtgc atctgtacca ggtcggcata
     1741 aacctttaac ggtgaggagc ctccgggaat tgccagaaca cagggtttgt tttgctcttc
     1801 ctttgcacgt ataagagctg aaatttcctt ggctacagcg agagcacctt ctttttcggt
     1861 ctcaaagatt tcagtacgta atttctcata acgtgactgt cgggtagctt ctaacggatc
     1921 tgttgaacga aaatactttt ccggtattcg ttccagtgta atttttgaac ttaaatcgaa
     1981 aatcataatt gaattttttt atagtatata ttggtatttt ttagatcatt actttgacta
     2041 ccagtttacg aatttttcct tcaccaataa tgggtgcatg agcatcttcg ggataaacaa
     2101 ttaaaaattc accgggttgt acggttatat aattgtccgg tttgccggca taaagagcgc
     2161 aatcatcctg actgttgtat tccgatttca gagttgaaca gtgttcgagt gctttaatac
     2221 ctattatttc ggctttatcg agcggaatat gcacatcgag atagcgacgg tgaacttcaa
     2281 gtacctgttc atcagcaggg agcaattgcg gattcacatt gttaataaac aaattatcgt
     2341 cgtcgagggt aatacgtccg atttcctgtc cgagaaaatc atgttccttc acgtagtcaa
     2401 agagttgttt gaatttcgga tgcaaacctt ctatgcgttt actttccgat aaagtggaat
     2461 aaatcattat tttatttttt tgatgttttt ataaattaaa ttaaaacaaa aaatgagata
     2521 aaaatgtttt gctcaatcgt cctgtttcag acttattttc catattttcg gaaatcggat
     2581 acctggtttt atttctccgt taacaacata aattaccatt atcgaaagtg accggaagag
     2641 ccggcaaagt gtcgatacgg agagtgttat caattcttat ccggtaaact gctgatgaaa
     2701 aaaaatcgtt atttcttcct ccgaaaaaca acaggccatt gggtattgta accgaaacgc
     2761 cataggcaag ttcacgcggg agttaacctg ttttttccca ttttttccca tagaactgaa
     2821 gtatatcttt atagaaaatt ttctttccac catcggctgc tggtacatcc ggaaaattac
     2881 aaccaccagc tacaatcaga ttatcatcaa tcagtccggc aaacgacgcg aaattgcctt
     2941 ttgatatatt tccaaactca gccggaacgg gataaaactc tgtcaaatat actcttaccg
     3001 gtttactttc agcttcctga tatgctccaa atgtaaatga aaacagcagt acaaatataa
     3061 ctaaatgaat tctcatttta atattcggaa tttattattt gacaatttct tttttattat
     3121 caggttttaa actttttcaa tgttgctgtt taaaacaata ggctgaataa tctgtttttt
     3181 taggtcgatt gaagtttatt ttatattctg aactttcgtt tttaaggtgg ttcgctgaag
     3241 agcgagtgca atgaatacta ttcacgcaga acggcaaatc caaatccagg atcaccgtta
     3301 tcggtcattt tacccaaaat actggtaact ccggcacggc aaacacgccc aatttcttag
     3361 ataattcaat ccgaaggtgt cagatatatt ttagactagt aaatcttctg gcatattttt
     3421 tttactccac ctgcctttta cattgatccg aaattatttc aaagccatcg acataaacat
     3481 caaaaaagca aaaaacccct gtcatcattc cgataacaga ggttctttaa taagtttagc
     3541 gacctgatta gtttcttgga gtagcttctt ttacaaccat ggtacgacca tcaagttctg
     3601 cgccgtttaa ttcagcaata gctttttttg cagcatcgtc gtcagccatt tcaacaaagg
     3661 caaaaccttt tgaacggcgt gtgtcgcggt cgatgataag cttgcaggaa tcaacctggc
     3721 catattcttc cataacctct ttaaggtcat tttctcttac tttgtaactt aagttaccta
     3781 cgtaaatgtt cataaaaaat aaaataatta aatgataatt aaataatgat taaagagaag
     3841 tgtaattacg caggtataac aaattctgat taaataatta gaagtgataa tagacagacg
     3901 aaaaatacat tttcttttcg gcgtcaaaga tatgcttttt atttttaatt cacaacaaat
     3961 tggtcaaaat aattgaaatt aaagaaatta tcttcaaaaa caggaataat cagatattat
     4021 tgatgaattg gaattttgtc aaaatgggat ttctgttgat tttttttgtg attatgaaaa
     4081 tatggcgttg ctatacgatt ataattaatt gagcttatcc tgacctttct ttatatctct
     4141 tctgaatacg tgattatgag gttattaaaa ggttacgatg actttccccc aaaaaaacct
     4201 ctcaataaaa acgttttcgt gtcaaccttg attaagctca acagacatcc tactttgaaa
     4261 attttggtgc acagtgactt tcacttatca cattagtgtg ggggtcttgt taataattcg
     4321 ttacgctgac acaccaaata ttcaagtgaa atttgtgtat gattcatttc aacataccca
     4381 atattacccg gagatttaca catgattcat ttcaacacac ccaatattcc agcgaaatga
     4441 ataacacgaa tagtacttaa aaaccaaccg gaaataaata gaaaagg
//
LOCUS       DAOPYB010000228         4466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_25140_length_4466_cov_6.158467, whole genome shotgun sequence.
ACCESSION   DAOPYB010000228 DAOPYB010000000
VERSION     DAOPYB010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25140_length_4466_cov_6.158467"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(467..2080)
                     /locus_tag="PKH58_12125"
     CDS             complement(467..2080)
                     /locus_tag="PKH58_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX89821.1"
                     /translation="MRNFNTHVLPLFLLLFFSPICLGNEPIAAGKFKPTWQSLEQYQV
                     PEWFRNAKFGIWAHWGPQCQPEQGDWYARFMYDEGSRQYKWHVENYGHPSKVGFKEVI
                     NDWKAENWDPEKLVKLYKRAGAQYFFAMANHHDNLDLWNSKYQEWNSTRVGPKKDILA
                     GWARAARNNNLPFGLSVHAAHAWTWMETAQRADKNGEFAGVPYDGKLTKADGKGTWWD
                     GLDPQELYAQNHVLSQGSENVNRIHSQWGWENGASIPSKEYCDKFYNRTVDMINQYNP
                     DLLYFDDTALPLYPASDVGLKIAAHYYNHNMATHNGKLRAVMFGKILTDEQKKCMVWD
                     VERGAPDKIQPVAWQTCTCIGDWHYRRSVYDDNRYKSAKNVILMLVDVVSKNGNLLLN
                     IPVRGDGSIDEKELAVVEGIAQWMAINKESIFDTRPWKIFGEGPAAEAVNPINAQGFN
                     EGKLKFSAKDIRFNHKGKTLYATVMGAPQEKITIMNLGKSGLQATIKKIELLGSREKL
                     IWRQDADKLSIEKPTRIPNDVAVVFKISF"
     gene            complement(2085..>4466)
                     /locus_tag="PKH58_12130"
     CDS             complement(2085..>4466)
                     /locus_tag="PKH58_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX89822.1"
                     /translation="MKKLPLILAFLFLTISTFAREKNLFNFDWKFQWGEIQDAYKTGF
                     DDSKWRNLDLPHDFQIEQPWDQSAGGARGFKAMGEGWYRKSFAANPDWKGKKVLLDFE
                     GIMLYGDVWVNGKKIGSTDYGYLGFEADISNILRYDTANVVAVYASTGKTGGSRWYTG
                     GGLFRDVNLIVKDPVSVARHGVFITTPIATAQQAAVSVQVEIEGFRNKENDLDITARI
                     FAPDGKLIGQTNSKAPVRSKSETKEIALPQLNITAPQLWSCESPALYTAEITLTLDGK
                     TVDKLQQKFGIRHIEFTQEEGFKLNGKKVFLKGISNHHDMGVVGAAAYETAIARQMDM
                     LKNFGYNHIRTSHNPYSEAFLRLADEKGILIVDELYDKWSDRDYWAGRKPWSVLWTEN
                     MTEWIKRDRNHPSVIMWSYGNELQMREDLAGYPTGDWGVTTYRMMDVLAKRYDPTRKS
                     TVAMFPARAGGVGKTDKDFNINILPPELATVTEVSSFNYRWFNYQDYLKHAPHMIIYQ
                     SEATTNELLAPYFGMDREKMVGLAYWGAIEYWGESNGWPKKGWNYSYFNHSLEPFPQA
                     YLIKSAFSDVPLVHIGVVDKESDKIEWNDQTIGTANVSSHWNREKGKSYNIYTYTNAD
                     EVELMINNKSVGIQKNNTEPDKRNIIRWQNIPFSAGKITAIARNAGKEVARHQLETTG
                     KAVALKLEPENQAWKANGMDLQYVKVYAVDSKGRKVFTASGELTFEVKGNAKLLAVDN
                     GDHYTDDIFTGNKVILHNGFAMVILRAGRTPGKVMLTTKTENFTSKTTELKTN"
BASE COUNT         1103 a         1050 c          910 g         1403 t
ORIGIN      
        1 ctttcacttc gcctattttt aacagccagt cggagttaaa gttgtatttt tcgcgtgctg
       61 aagtgccgaa agagaagctg cagaataaga taagtacgaa aatagattta agaattttca
      121 tatgctgata atttatatct gccccctaat ccgtcagctg acggaggaat atagagcgtc
      181 tatcatttgt tgtacgattt tttaatcatg cccgtgtatg tcaaaaatat attacccgta
      241 tatgtgtttt tcatgtgttt aatttttatt cttttcatta aggacatatg aaatacgcct
      301 tacgttagtc atcggaacat ctccatgtgt gtctaagtta tttttacatg gctgtttcat
      361 tttattgttt gattagaaga tgttctgata atgtgtattt cgtttaataa gaattgtaga
      421 ttttgtgtgc ctgctaatac ctcaaagatt tatttcttca tgggtattag aaactgattt
      481 tgaaaaccac tgcaacgtca ttcggtatac gggttggttt ttcgatggat agcttatcgg
      541 catcctgtct ccaaatgagt ttttcgcggc ttcctaataa ttcaattttt ttgatggttg
      601 cctgtaatcc cgattttccc aaattcatta tcgtaatttt ttcctgaggt gctcccatta
      661 ccgtagcata aagcgttttt cctttatgat tgaagcgaat atctttggct gagaatttca
      721 gttttccttc gttaaatccc tgtgcattta tggggttcac tgcttctgct gccggacctt
      781 cgccgaatat cttccacgga cgggtatcaa atatactctc tttgttaatg gccatccact
      841 gcgcaatacc ctcgacaaca gccaattcct tttcatcaat gctgccatcg ccccgaacgg
      901 gaatattcag taataggttt ccatttttac ttaccacatc caccaacatc aaaatcacgt
      961 ttttagctga tttgtagcga ttatcatcat acaccgatcg ccggtagtgc caatcgccta
     1021 tacaggtaca ggtttgccag gctacgggtt gaattttatc gggcgcaccg cgttccacat
     1081 cccacaccat gcattttttt tgctcatcgg ttagtatttt accaaacatt acggcccgga
     1141 gtttgccatt gtgagtagcc atgttgtggt tgtaatagtg cgctgcaatt ttcagaccca
     1201 catcgcttgc cggatacaaa ggcaaggctg tatcatcgaa atacagcaaa tccggattgt
     1261 attggtttat catatccact gtgcggttat aaaatttatc gcaatattcc tttgaaggta
     1321 tcgaagcccc gttttcccac ccccactgac tgtgaattct gtttacattc tcactaccct
     1381 ggctaagtac atggttttga gcgtacagct cctgaggatc caaaccgtcc caccaagtac
     1441 ccttgccatc ggctttggtc aatttgccat cgtatggtac tccggcaaac tcaccatttt
     1501 tgtcggcccg ctgtgctgtt tccatccacg tccaggcatg ggcagcgtga acacttaaac
     1561 caaaggggag gttgttgtta cgagctgcac gagcccaccc ggccaaaata tcttttttcg
     1621 ggcctacgcg tgtagaattc cattcctggt atttactgtt ccataaatcg aggttgtcgt
     1681 gatgattagc catagcaaaa aaatactgcg caccggcacg tttgtagagt tttaccaatt
     1741 tttcgggatc ccagttttct gccttccaat cgttaatgac ctctttgaaa cccacttttg
     1801 aaggatggcc gtaattctcc acgtgccatt tgtattgcct gctgccttca tcgtacataa
     1861 accgggcata ccaatcgccc tgctcgggct gacattgcgg tccccagtgc gcccatatac
     1921 cgaacttggc attccgaaac cactcaggga cctgatattg ctcaagtgac tgccaggttg
     1981 gtttgaattt gccggcagcg atgggttcat tgcctaaaca aatgggagaa aaaaagagta
     2041 atagaaatag gggaagtaca tgagtattaa aatttctcat tacattaatt cgttttaagt
     2101 tctgttgttt ttgatgtaaa attctctgtt ttggtagtca gcatcacttt tccgggcgtt
     2161 cttccggctc tcagaatcac cattgcaaat ccattgtgca gtattacctt gttaccggta
     2221 aaaatatcgt cggtataatg gtcgccgtta tccacagcca gcaattttgc atttccctta
     2281 acttcaaaag taagctctcc ggaggctgta aacactttcc gccctttgct atccaccgca
     2341 tacactttca catactgcaa atccatgcca ttggctttcc atgcctgatt ctcaggttcg
     2401 agtttcagtg caacagcttt tccggtagtt tccaactggt gacgtgcaac ttcttttcct
     2461 gcatttcggg ctattgctgt aattttacct gccgaaaagg ggatattttg ccagcgaata
     2521 atattccttt tatcgggttc agtattattt ttctgaatac caaccgattt attattgatc
     2581 atcaattcca cctcatcggc attggtatag gtataaatat tgtaggattt ccccttttcg
     2641 cggttccagt gcgacgaaac attggccgta ccaatagttt ggtcgttcca ctctatttta
     2701 tcgctttctt tatccaccac cccgatatgt accaacggca catccgaaaa agcgctttta
     2761 atcagatacg cctgcggaaa tggctccagg gaatggttga aatatgaata attccagcct
     2821 ttttttggcc agccgttgga ttcgccccag tattcaatgg ctccccaata ggccaggcca
     2881 accattttct ctctgtccat tccaaaatac ggcgccagta attcgttggt ggtagcttcg
     2941 ctctgataga tgatcatgtg cggtgcatgc ttcaggtaat cctgataatt gaaccagcgg
     3001 taattgaaac tggaaacttc ggtaacggta gccaattcgg gcggaagaat attaatgttg
     3061 aagtctttat cggttttgcc tacaccacct gcccgggccg ggaacatagc cactgttgat
     3121 tttcttgtcg gatcgtagcg ttttgccaaa acatccatca ttcggtaggt ggttaccccc
     3181 caatcgccgg taggatagcc tgccaaatct tcacgcattt ggagttcatt gccataactc
     3241 cacatgataa ccgatggatg attgcggtcg cgtttgatcc attcagtcat attttcggtc
     3301 cacaaaaccg accacggttt acggcctgcc cagtaatccc tgtcgctcca tttatcatac
     3361 aattcgtcaa cgatgagaat acctttttca tcggccaacc ggagaaatgc ttccgaatac
     3421 ggattgtgtg acgtacgaat atgattatag ccaaagtttt tcaacatatc catctggcgg
     3481 gcaatagcgg tttcgtaagc agcagcacct acgacaccca tatcgtgatg attggagata
     3541 cctttcagga aaaccttttt accattgagt ttaaatcctt cttcctgtgt aaattcaata
     3601 tgacggatgc caaatttctg ttggagttta tccaccgttt tgccatcgag tgtgagggta
     3661 atttctgccg tgtaaagtgc cggactttcg cacgaccaaa gttgtggagc tgtaatattg
     3721 agttggggca atgcaatttc tttggtttca ctttttgaac gtacaggtgc tttgctattt
     3781 gtttgaccaa ttaattttcc atcgggtgca aaaattcgcg cagtgatatc caggtcgttt
     3841 tctttattcc ggaaaccttc aatttccacc tgcacactga cagcggcctg ttgcgctgtg
     3901 gcaatgggtg tagtaataaa aacaccgtga cgggcaacgg aaaccggatc ctttacaatc
     3961 aggtttacat cgcgaaacaa gccaccaccc gtgtaccaac gcgaaccgcc tgttttgccg
     4021 gtggaggcat acacagctac tacattggca gtatcgtagc gcaatatgtt cgaaatatcg
     4081 gcctcaaagc ccaggtagcc gtaatcggtg ctgccgattt ttttgccatt gacccacaca
     4141 tcgccataca gcataattcc ttcaaaatcg agcagcactt ttttcccttt ccagtcgggg
     4201 tttgctgcaa agcttttgcg gtaccagcct tcgcccatgg ctttgaaacc gcgtgctccg
     4261 ccggcagatt ggtcccaggg ctgttctatc tgaaaatcgt gcggtaaatc aagatttcgc
     4321 cacttgctat catcgaaacc ggttttgtag gcatcctgaa tttcgcccca ctgaaatttc
     4381 cagtcgaaat taaatagatt tttttcacgg gcaaatgtgg aaatcgtcag gaacaaaaaa
     4441 gctaaaatta atgggagttt tttcat
//
LOCUS       DAOPYB010000229         4463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_25171_length_4463_cov_5.454855, whole genome shotgun sequence.
ACCESSION   DAOPYB010000229 DAOPYB010000000
VERSION     DAOPYB010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25171_length_4463_cov_5.454855"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2126
                     /locus_tag="PKH58_12135"
     CDS             <1..>2126
                     /locus_tag="PKH58_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX89823.1"
                     /translation="AALLDEVVITGYTGTQVRSRSTNSIAKVDNKKLTVGVFSNPGQA
                     LSGAVSGLRVTQTSGNPGATPSIVLRGGTNLDGSGAPLVIIDGQVRGSLSDINPEDIE
                     DMQVMKDAGATAIYGARANNGVILITTKKGKSGQSEINFKAKVGVNYLNNAYEFADAA
                     TYLTRMRTAYVKSASIWTKADGTDQGYAGLAGLTGTQPYGTGNAYFAADGVTPLVNTT
                     NSIWSTMYYDPKYDFLLNQGWQKMRDPVWDYMKKSNPNAGEYLIFKNTTPADYNFVNP
                     SMSQDYNLNMSGGNDKGHYYGGIGYNYSEGIPITSFYKRYSFLFNGDYKIKPWLTSIS
                     SFSYGRANWQSMPGSQTDEANYFSRILSVPPTVRFTNEDGTPRFGNNASDGNQSFQPE
                     KFFRFNQTDKFTMNQAFKVDFSKSLYLRMAANWYYSEGFSESFNKDYQTSPTAFNRDR
                     SSYAGFSRTLDQTYNAVLNYEKQFNEHYLAVMLGSEYYDSFNYGIDASGSGAATDDFR
                     DLSLTSADKDKRSIDSYHTQQRILSFFGRANYDYMGRYILSAVVRRDGYSRLLDNRWG
                     TFPGISAAWMFSKESFMANQNIISFGKLKASYGLNGNVSGVGAYELQGSYVGSSKYNG
                     TSTFVLGSVPNPGLRWEKSRTLEMGLDLSYLQNRFSTNFTVYDRLTSDKFASLSLPIS
                     SGISSIRTNNGSFRNRGLEIELAAKP"
     assembly_gap    2127..2226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2227..2766
                     /locus_tag="PKH58_12140"
     CDS             <2227..2766
                     /locus_tag="PKH58_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HNX89824.1"
                     /translation="GDNIIDDYDQVKAGNTTPHWFGGFNSTLNYKNFSLYAAFDYALG
                     FYMMDYRLPWILANAQGTYNMTTDVNDTWSPDNPNAKYPTYQWADQLGKGNYRNSTIF
                     TYRGDYLSFRQVSLSYSFPRTLINKAKIEKLDLSVTGQNLGYLTAAKTLANPEKGGGI
                     QDAGYSLPRILTFGLNITF"
     gene            2798..4402
                     /locus_tag="PKH58_12145"
     CDS             2798..4402
                     /locus_tag="PKH58_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX89825.1"
                     /translation="MKKIIMKYISAATLGLALTFTACDSLNLGPIDYYGNANYWQTVP
                     QVQAYVNGMHLDLRSTHFTRDFILGEARGGLQVTGTSSQNVSTSNDDIKGNKISGDIP
                     GVSQWGGAGSVYGNVFDCNLLIQNIEDSEMHKSNKATVDFLLAQTYGIRAFHYFKLYR
                     AYGGVPLVNKVKVLDGQVTADELYTGRSTPKETMDFIKADLKKSLDYFASAGKDWVNN
                     VTWSKAATQMLAAEVYLWSAKVSLGNQTPAAGDLAAAEGYLNDVKNNTSFGLLTDFDK
                     IFASNNKANKEIILAINYADGEATSDVSLFLYADASINLFYDKAGVRLGDPLNLKNTG
                     IQRYEYTLDFWKSFANKDKRRAATFMEYYDNKGVVKGTVMKKFVGSINTTGSRVYDCN
                     EPIYRYADVLLMLAEVENMKSGNPAQYINQIRQRAYGTDYVAATDAFVNADFKTNEYA
                     ILQERDKEFVYEGKRWYDIVRMKDALTGQPLAFDAASAYTTTPVLKSTERYKVLWPID
                     KATLNADPLLKQTPGYKVADQVEEVW"
BASE COUNT         1320 a          938 c          934 g         1171 t
ORIGIN      
        1 acgcagcttt gctcgacgaa gttgtaatta cggggtacac aggtacacag gttcgttccc
       61 gaagcaccaa ctccattgcc aaagtagata acaagaaatt gaccgtcggg gtattctcca
      121 atcccggaca ggctttatct ggtgccgtat ccggtttacg cgttacacaa acctccggta
      181 acccgggcgc aactccaagc atcgtacttc gcgggggaac taacctcgat ggcagcggcg
      241 ctccgctggt catcattgat ggtcaggttc gcggtagtct tagcgacatc aaccccgaag
      301 acatagaaga catgcaggtg atgaaagacg caggtgcaac tgccatctac ggtgcccgtg
      361 ccaataacgg tgtaatcctt attaccacca aaaaagggaa aagcggacag tcagagatca
      421 acttcaaggc aaaagtcggt gttaactacc tgaacaatgc ctatgaattt gcagatgcag
      481 ctacctattt aacaagaatg cgtactgctt atgtgaaatc ggcctctatt tggacaaaag
      541 cagatggtac cgatcagggc tatgcaggcc tggcaggcct taccgggaca caaccatacg
      601 gaacagggaa tgcctatttt gctgctgacg gggttactcc attagttaac actacaaact
      661 caatatggag tactatgtat tacgatccca agtatgattt ccttcttaat cagggatggc
      721 aaaaaatgcg tgatccggtc tgggattata tgaagaaatc aaacccaaat gctggtgaat
      781 atctgatttt caaaaacacc actccagcag attacaactt tgtaaaccct tccatgtcac
      841 aggactacaa cctgaacatg tctggaggaa atgataaagg tcactactat ggaggtattg
      901 gttacaacta ctctgaaggt attcctatca cttcattcta caaacggtat tctttcctgt
      961 ttaacggtga ttacaagatt aaaccctggc tgacttctat ttcaagtttc agctacggtc
     1021 gcgccaactg gcaatccatg cccggttcgc aaacagatga agctaattat tttagccgca
     1081 tactttcggt accacctacc gttcgcttca ccaacgaaga tggtacaccg aggtttggta
     1141 acaatgcaag tgacggaaat caaagcttcc agcccgaaaa attcttccgg tttaatcaga
     1201 cagacaaatt caccatgaat caggcattca aagttgattt ttcaaaatcc ctctacctga
     1261 gaatggcagc caactggtat tacagcgaag gtttcagtga aagtttcaac aaggactatc
     1321 agaccagccc aactgccttc aacagagaca gaagctctta tgccggattc agcagaacat
     1381 tggatcagac atacaacgca gttttaaatt acgaaaaaca gtttaatgaa cattatttag
     1441 ctgtcatgct gggttctgag tattatgaca gttttaatta tggcatagat gcatccggtt
     1501 caggtgctgc taccgacgac ttccgtgacc tttcgctgac ctctgccgat aaagataaaa
     1561 gaagcattga cagctatcat acacaacagc gcattttatc cttcttcggt agggcaaatt
     1621 acgactatat gggtagatat attttatcgg ccgtagtacg tcgtgacggt tattcccgat
     1681 tacttgacaa tcgctggggt acattccccg gtatttctgc agcctggatg ttctccaaag
     1741 aatcgtttat ggctaatcag aatattatct catttggtaa gctgaaagcc agttatggtt
     1801 tgaatggaaa cgtatcgggt gtgggtgctt atgaattgca gggaagttat gttggctcga
     1861 gtaaatacaa tggaacttca acatttgtgt taggatctgt accaaacccc ggtcttcgtt
     1921 gggaaaaatc acgtacgctg gaaatgggtc tggatttgag ctatttacaa aacagatttt
     1981 caacaaactt caccgtgtac gatcgcttga cttcggataa atttgcatca ttaagcctgc
     2041 caatttcatc aggtatcagc agtattcgta ccaacaacgg ttcattccgc aacagaggtc
     2101 ttgaaattga actggctgca aaaccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnggtg ataatattat tgacgattac gatcaggtaa aagcaggtaa tacaacaccc
     2281 cattggtttg gtggttttaa ttcaacgtta aactacaaaa atttctcgtt gtatgcagct
     2341 tttgactatg cacttggttt ctacatgatg gattaccgct taccctggat acttgctaat
     2401 gctcagggca cctacaacat gactaccgac gtgaatgata cctggtcacc ggataatccg
     2461 aatgccaaat atcctacata tcaatgggct gatcagttag gaaaaggaaa ttaccgcaat
     2521 tctacaattt tcacctacag aggcgattat ctctcattcc ggcaggtttc cctttcctat
     2581 tcattccccc gtacactgat caacaaagcc aagattgaaa aactggatct ttcggttacc
     2641 ggtcagaact taggatactt aactgctgcc aaaacgttgg ctaaccctga aaaaggtggt
     2701 ggtatacagg atgcaggata tagcttacca cgtattctta cattcggatt aaacattact
     2761 ttctaagtca cattaactat ataacaccaa tattaatatg aaaaaaataa taatgaaata
     2821 tatatcggca gcaacgctgg gattagctct cacatttact gcctgcgaca gccttaatct
     2881 gggtccaata gactattatg gcaacgccaa ctactggcag actgtgccac aggttcaggc
     2941 atatgttaac gggatgcatc ttgacttgag aagtacccat tttacccgcg attttatcct
     3001 tggggaagct cgcggaggcc tccaggttac cggtaccagc tcacagaatg tatcgacatc
     3061 gaatgatgat attaaaggaa ataaaatttc gggcgacata ccgggagtga gccaatgggg
     3121 cggtgccgga agtgtttatg gcaatgtttt tgactgcaac ttactgatcc aaaatataga
     3181 agacagtgaa atgcataaaa gcaacaaagc aaccgttgac tttctgttag cacaaaccta
     3241 tggcatccgg gcatttcact atttcaaact gtatcgtgcc tacggtggag ttccactggt
     3301 taacaaagta aaagtacttg atggtcaggt aactgccgat gaactttata ccggtcgctc
     3361 aacaccaaaa gaaacgatgg actttatcaa agccgatttg aaaaaatcgc tggattattt
     3421 tgcttctgcc ggtaaagatt gggttaataa tgtgacctgg tcaaaagcag ccactcaaat
     3481 gctggcagcc gaagtgtatc tgtggtcggc taaagtttca ctgggcaatc aaactcctgc
     3541 agccggcgat ttggcagcag ctgaaggtta tctgaatgat gttaagaaca ataccagttt
     3601 cggtttactg actgattttg acaaaatttt cgccagcaac aataaagcta ataaagaaat
     3661 cattttagca ataaattacg ccgatggtga agccacaagc gatgtatcct tattcttata
     3721 tgctgatgca agcatcaacc tgttttatga taaagccggg gtacgcctgg gtgacccttt
     3781 aaatctcaag aatacaggta ttcaacgtta tgaatacaca ctcgatttct ggaaaagttt
     3841 tgccaataaa gacaaaagac gtgctgccac ttttatggag tattatgaca ataaaggagt
     3901 ggttaaagga acggtgatga aaaaatttgt gggatcaatc aatacaaccg gttcacgtgt
     3961 gtatgattgc aacgaaccta tctatcgata cgccgacgtg ttacttatgc tcgccgaagt
     4021 ggagaatatg aaaagtggaa atcctgctca gtacatcaac cagattcgtc agcgcgctta
     4081 tggcactgat tatgtagccg ctacagacgc ttttgtcaat gccgatttca agaccaatga
     4141 atatgcaatc ctgcaggaaa gagataaaga atttgtgtat gaaggcaaac gctggtacga
     4201 tattgtacgc atgaaagatg ctctaacagg acagccgctg gcatttgatg ctgcttcggc
     4261 atataccacc acaccggtat tgaaatctac tgaaagatac aaagtgctgt ggccaattga
     4321 taaagctacc ctgaatgccg atccgctgct caaacagacc ccgggatata aagtggctga
     4381 tcaggtagaa gaggtttggt aattagtaag ataatccaat ttaattaaag gagttgaatt
     4441 cattttcaac tctttttttt att
//
LOCUS       DAOPYB010000230         4394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_25732_length_4394_cov_9.376124, whole genome shotgun sequence.
ACCESSION   DAOPYB010000230 DAOPYB010000000
VERSION     DAOPYB010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25732_length_4394_cov_9.376124"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..2330
                     /locus_tag="PKH58_12150"
     CDS             57..2330
                     /locus_tag="PKH58_12150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX89826.1"
                     /translation="MNIKKAGLLILISLLIMNVNAQSLSGDWKGTLSVHGTNLELIFH
                     ITEKDGAYTSTMDVPAQGATGIPVQKTEFIGNTLKISVPAAQINYSGELTGDSITGNF
                     EQMGMKLPLTLKKATIKLPGNTALVSSDETLKKLVESDNGNYKYKVSDYFARPKASSF
                     QLSPNGKYMSYMEKDDKNKNHVYVKEIASGKVNRAIEEKDELIKGYGWISDDKLFYAM
                     DKGGNENFHIYVADIDGQTPKDMTPFDGVRAGIITILKDQKNIMIIQMNKNNKQIFEP
                     YRLNAATGELTQLFENKDAANPIQGYDFDKDGNLRAYTKMVNGVEMELYYKDLSTNEF
                     KLVTRNKWDDNFGIISFSYNPNNKDEAYVYSNLDSDKARIFLYDFKTNKKIKEVFSNP
                     NYDVTGMHLSRKRNYELDYFSYDGEKSVIIPISKIYKDLDSRIKKQFPGKEYGVVDFD
                     DDETKFLVILQSDKLYGTYYEYDTKTKTFKLLYDLMPQLKEADMAVMKPITFKSRDGM
                     TIHGYITLPKEALAGNKVPMIVNPHGGPQGIRDSWGFNPETQLFASRGYATLQVNFRI
                     SGGYGKEFLRAGFKQIGRKAMDDVEDGVKYAISQGWADKDKIAIYGGSHGGYATLMGL
                     VKTPELYACGVDYVGVSNIETFFASFPEYWKPMKDMVKEIWYDLDNPEELKIAREVSP
                     VNNIDKIRKPLFVVQGANDPRVNINESDQIVTALRAKGFEVPYMVKYNEGHGYYREEN
                     RMDFYGAMMGFFAKYLK"
     gene            2476..2548
                     /locus_tag="PKH58_12155"
     tRNA            2476..2548
                     /locus_tag="PKH58_12155"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2509..2511,aa:Thr,seq:cgt)
     gene            complement(2705..3478)
                     /locus_tag="PKH58_12160"
     CDS             complement(2705..3478)
                     /locus_tag="PKH58_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNX89827.1"
                     /translation="MRKLKTTLILITLLLSISAYSQSKKEYKLVWKDNFSGKTIDTTA
                     WTHEEAKPGWVNNEKQRYTNGENAVQKGGKLILTAQHENNEYTSSRLITRNKRIFTHG
                     FFEIKAKLPAGKGTWPALWMLGNNIDQVGWPASGELDIMEHVGRDPGVVFSSIHNTSG
                     YGATPYSGKVIIERPYDKYHIYAMEWTPHYITFFVDGRQIYHYKPELKTPENWPFDKP
                     AYFIFNIAIGGNLGGEIDNSIFPTTMIIDWVKVYQRSEY"
     gene            complement(3491..4348)
                     /locus_tag="PKH58_12165"
     CDS             complement(3491..4348)
                     /locus_tag="PKH58_12165"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX89828.1"
                     /translation="MKKIIVTILVLVPFFMFSQKEIKLFPQGASEGVFSPQTEAANGV
                     EWITFVSEARMYMYPSISQNKSGTSVLICPGGGYGGLAAEKEGAEIARWFNSIGVTAF
                     VLYYRMPFGNHQIPLKDTKTAMRIIRRHAKEWKLNKNRIGIIGFSAGGHLASTLGTHY
                     DKENKPLFMALIYPVISFRPVFFPGGTCQNLLGENPAEDLINFFSNELHITRQTPPTF
                     LVHALNDDVVEPYHSQAFADLLKVKKVKHKIVLYNQGGHGFGMRPQKVDADNWPLEFK
                     EWLKKYKFI"
BASE COUNT         1438 a          864 c          827 g         1265 t
ORIGIN      
        1 tataaaaaat cacacatcaa ttcgcatatt aagtaaaaat cagaaaaaaa attattatga
       61 acatcaaaaa agcaggatta ttaatcctaa ttagtctttt aattatgaat gtaaatgcac
      121 aaagcctctc aggcgactgg aaaggaacct tatctgttca tggtacgaat ctggaactga
      181 ttttccacat taccgaaaaa gacggcgctt acacttccac aatggacgta cccgctcagg
      241 gtgcaacagg aattccggtt cagaaaaccg aatttatcgg caataccctt aaaatcagcg
      301 tccccgctgc tcagattaat tattccggag aactcacagg ggacagcatt accggaaact
      361 ttgaacaaat gggaatgaaa cttccattga cactaaaaaa ggcaactatt aaactgcccg
      421 gaaataccgc ccttgtttcg tcggatgaaa cattgaagaa actggtggaa agtgataacg
      481 gaaattataa atataaagta tccgattact ttgcgcgacc aaaagcttcg tcctttcagc
      541 tttcgcctaa cggcaaatac atgtcgtata tggaaaaaga cgacaagaac aaaaaccacg
      601 tgtatgtaaa agaaattgct tccggaaaag tgaaccgcgc catcgaagaa aaagatgaac
      661 tgataaaagg ttacggctgg ataagcgatg acaaactctt ttacgcaatg gataaaggcg
      721 gaaatgagaa tttccatatt tatgtggcag atattgacgg tcagactcca aaagacatga
      781 caccttttga cggcgtaaga gcaggtatta ttaccattct gaaagatcag aaaaatatca
      841 tgatcatcca gatgaacaaa aacaacaagc agatttttga gccttaccgc ttgaatgccg
      901 caaccggtga gttgacgcag ttattcgaaa acaaagatgc cgcaaatcca attcagggat
      961 atgactttga taaagacgga aatctgcgtg catataccaa aatggtcaat ggcgtggaaa
     1021 tggaattgta ctacaaagac ctttcaacca atgagtttaa attggtgaca cgcaataaat
     1081 gggacgacaa cttcgggata ataagcttca gctacaaccc gaacaataaa gatgaagcct
     1141 atgtgtacag caatctggac agcgataaag cccgcatctt cctttacgat ttcaagacca
     1201 ataaaaagat taaagaagtt ttctccaatc cgaactatga tgtgacaggc atgcaccttt
     1261 cacggaaacg caattacgag ctggattatt tttcatacga tggcgaaaaa tcagtgatca
     1321 ttccaatcag caaaatttat aaggatttag acagccggat aaaaaaacaa tttcccggaa
     1381 aagaatacgg tgtggtagat tttgacgatg atgaaacaaa gtttttggtt attctgcaaa
     1441 gtgacaaact ctatggaact tactatgagt acgataccaa aacaaaaaca ttcaaacttc
     1501 tttacgatct gatgcctcaa ctgaaagaag ctgatatggc agtgatgaaa cccatcacct
     1561 ttaaaagccg cgacgggatg acgatacacg gctacatcac actaccaaaa gaagcattgg
     1621 caggaaataa agttccgatg attgtaaacc cacacggagg tcctcagggt attcgcgaca
     1681 gttggggttt caatcccgaa actcagcttt ttgcaagccg cggttatgca acgttgcagg
     1741 ttaatttcag aatttcaggc ggatacggaa aggaattttt aagagcagga ttcaagcaaa
     1801 taggccgtaa ggctatggat gatgttgaag acggtgtaaa atatgccatt tcgcaaggct
     1861 gggccgataa ggataaaatt gctatctacg gtggtagtca cggtggttac gccaccttaa
     1921 tgggattggt gaaaacacct gaattatatg catgtggcgt tgattatgta ggcgtttcca
     1981 atatcgagac attttttgcc tctttccctg aatactggaa accgatgaaa gatatggtaa
     2041 aagaaatctg gtacgatctg gacaatcccg aagaactgaa aattgcccgc gaagtatcac
     2101 cggtcaataa tatcgacaaa atcaggaaac cattatttgt tgttcaggga gctaacgatc
     2161 cacgcgttaa catcaacgaa agtgaccaga tagttaccgc cctccgtgca aaaggattcg
     2221 aagttcctta tatggtgaag tataacgaag gacacggata ttatcgtgaa gaaaaccgca
     2281 tggattttta cggagctatg atgggtttct ttgctaaata cctgaaataa atcaggttaa
     2341 aaacagtcat taataaaccg gacacttgca cgaaagcggg agtccggttt ttatttttct
     2401 caaaattaaa aatgagacaa agagttcttt tcagattaca gaaaaaattc tatctttgca
     2461 atcgtttttt aacaagcctt tttagctcag ttggtagagc aattcattcg taatgaatag
     2521 gtctggggtt cgagtcccca aaaaggctca caataattat ctgcaaaatt ctcatatttt
     2581 ataaaaaata tgtattaaca atccagcaaa aaatatctat tcacatattt gatattttcc
     2641 tttcccttct catatttaag attatacaag ttatagatta ttctcaagga tgtctctata
     2701 tattttaata ctcacttctt tgatatactt ttacccaatc aataatcata gtagttggga
     2761 aaattgaatt atcaatttct cctcccaaat tacctccaat tgcaatatta aaaataaaat
     2821 atgccggctt gtcaaatggc cagttttctg gtgtttttag ttcaggttta tagtgataaa
     2881 tttgtcgtcc atctacaaaa aaggttatat agtgcggcgt ccattccatg gcatagatat
     2941 gatatttgtc gtagggtcgt tcaataatga ctttaccact gtaaggagtt gcaccatagc
     3001 ccgaagtatt gtgaatggaa gaaaacacaa ctccgggatc tcttcctaca tgttccatga
     3061 tatctaattc gccactggca ggccagccta cctgatcgat attattgccc aacatccaca
     3121 acgcgggcca ggtgcctttc ccagccggta gtttagcttt tatttcgaaa aaaccgtgag
     3181 taaaaatacg tttatttctg gtaatcagac gggatgaagt gtattcgtta ttttcatgtt
     3241 gggcagtaag tatcagctta cctccttttt gcacagcatt ttcaccattt gtatatctct
     3301 gtttctcgtt attgacccaa cccggctttg cctcttcatg tgtccatgcc gtggtatcaa
     3361 tggttttgcc actgaaatta tctttccata caagcttata ttctttttta gattgtgaat
     3421 aagcagaaat actaagcaat aatgtaatca aaatcaatgt tgtttttagt tttctcatag
     3481 tattatagtt ctaaataaat ttatattttt tcagccattc cttaaattct aaaggccaat
     3541 tgtcagcatc gaccttttga ggtctcattc caaatccatg accgccctga ttgtaaagta
     3601 caattttgtg tttcactttt ttcactttca atagatcagc aaatgcctga ctatgatatg
     3661 gctccaccac atcatcgttt agcgcatgaa ctaaaaatgt aggtggagtt tgcctggtaa
     3721 tatgtaattc gtttgaaaag aaattaatta aatcttctgc aggattttca cccaacaaat
     3781 tttgacacgt tccacccgga aaaaagacag gacgaaaaga aattaccgga tatatcaaag
     3841 ccataaataa tggtttattt tctttatcgt aatgtgttcc aagtgtggaa gccagatgtc
     3901 cgcctgcgga aaaaccaatg atacctattc tgttcttatt gagtttccac tcctttgcat
     3961 gtctacgaat aatgcgcatg gcggttttag tgtctttcaa aggtatttgg tgattcccga
     4021 agggcatacg gtaataaagc acaaatgccg taacgccaat actattaaac caacgggcaa
     4081 tttcggctcc ttctttttca gcagccagtc caccataacc accacccggg caaatcagca
     4141 ctgaagtccc acttttattt tgtgaaatag aaggatacat gtacatgcgg gcttcactta
     4201 caaaagtgat ccactctacc ccattggcag cttcggtctg aggactaaaa accccttcgg
     4261 atgcaccttg cgggaacagt tttatttctt tttgtgaaaa cataaaaaat ggcacaagca
     4321 ctaatattgt tacaattatt ttcttcattg aattaaaatt tattctaacg tttttttata
     4381 cttatttccg aatc
//
LOCUS       DAOPYB010000231         4358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_26055_length_4358_cov_7.680920, whole genome shotgun sequence.
ACCESSION   DAOPYB010000231 DAOPYB010000000
VERSION     DAOPYB010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26055_length_4358_cov_7.680920"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /gene="trkA"
                     /locus_tag="PKH58_12170"
     CDS             <1..1130
                     /gene="trkA"
                     /locus_tag="PKH58_12170"
                     /inference="COORDINATES: protein motif:HMM:NF007038.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNX89829.1"
                     /translation="IAVTPFESVNMTACMLATNLGAKKTLARIENYEYLQPANKEFFE
                     KLGVDYLICPEKLAAEEIVEALKHTWLRQNLSFCNDALILLGIKVRNNSEIIDKPFNS
                     GYFDHGKYRVVAINRKEKTIIPSGYDQISAGDIVYFITMKPNVEFVREQAGKIVYPVR
                     NVMIMGGSRIAQKTIQYLPGNINTKILERDKEKCYFLAEKLEDTLIINADGRNVDALR
                     SEGIQEIDAFVATTANSEANILACLEAKRLGVKKTVAEVENIDYIQLAEDLDIGSVIN
                     KKMIAASYIYQLTLDADVLDVRSLTSADAEVVEFVAKEGSKITKTQIKNIRLPENVNI
                     GGIVRDGVGSIVYGNTQIMPNDHVIVFCISSAIRKVETLFN"
     gene            1216..2655
                     /locus_tag="PKH58_12175"
     CDS             1216..2655
                     /locus_tag="PKH58_12175"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNX89830.1"
                     /translation="MKIVGGLLLVEAVFLLFPLLVTLIYGEAESRFYLRAIGISAGLG
                     LVGLLSGIKAEMFFGKREGAVIVTLTWLMFTLIGALPYWLSGNIPTFTDAFFESLSGF
                     TTTGTSILNNIEALPYNMLFWRSMTHWIGGLGIMVMFLALIPFLGGAGYELFSSEGTT
                     PIKEKITPKIRETAKFLLLVYILLTALCTIALTLAGMSLFDAVNHALSTISTGGFSTK
                     QLSIAYWNSPAIEYVIIFFMFFSGINFTIFYYIYKSNWDRIKKNEELKYYILIFLVSV
                     LIIIYSHIDFSKPLTFGSIEKSVRGSLFIVTSCITTTGLCTEDYTLWSSYTWIVLLVL
                     MVTGASTYSTSGGIKIARVVVLAKFCYSEFKKLIHPNAVIPVRFNGYIVKDDLLLRVL
                     AFSIIYLLIIVTGTFILSLTGLDFEEAISGMITCMSDVGLGLGKLGPSSSFAEIPVVS
                     KWFLAFVMLLGRLEIYSVLLLFTPVFWKK"
     gene            2669..4141
                     /locus_tag="PKH58_12180"
     CDS             2669..4141
                     /locus_tag="PKH58_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNX89831.1"
                     /translation="MFKSEINYKLVSRVIGNLLLVESAVIFFVLFITLIYGEKDGWYF
                     VGTSVLALLAGLLMIVFGKNAPDKIGKREGSMIVTFTWIIFSLIGALPFWLSKSIPSF
                     TDAFFETISGFTTTGASILNNIEELSHGMLFWRSLTHWVGGLGIIVISLALLPVFGVS
                     GTQLFAAETTGPTKDKIHPKLNETAKRLFLIYIILTAAETIFLLFGRMSLFDAVCHSF
                     STIATGGFSTKQASIAYYNSPYIEYVITIFMILSGVNFGNYYLAYQRKFDKIKENEEL
                     RFYLIVLFLFTVIVALSLIDFSEVVTGSSVEKAWREAFFTVSSLMTTTGMVTADYMFW
                     KPLTWIILITVMMTGASAGSTSGGIKMIRVVISAKACYYEFKRLIHPTAVIPVRYNKH
                     VVREDTLTRVIAFTLLYIVFALFGILVLAISGMGFLESIGGMVTCLGGVGPGLGLVGP
                     TGNFAQIPEFSKWFLDLVMLTGRLELYTVLMLFTPAFWRK"
BASE COUNT         1193 a          833 c          943 g         1389 t
ORIGIN      
        1 tcattgctgt tacacctttt gaaagtgtaa atatgactgc ctgcatgctg gcaaccaatc
       61 tcggtgctaa aaaaacactg gcgcgaattg aaaactatga atacctgcaa cccgcgaata
      121 aggaattctt cgaaaaactg ggtgtggatt atctcatttg tcccgaaaaa ctggcagctg
      181 aagagattgt ggaagcccta aagcatacct ggcttcgtca gaacctgagt ttttgcaatg
      241 atgccctgat cctgctgggt attaaagtta gaaataattc ggagattatt gataaacctt
      301 tcaattctgg atacttcgat catggtaaat acagggtggt agccatcaac cgcaaggaga
      361 aaacgattat cccaagcggc tacgatcaaa tatcagccgg cgatatcgtt tattttatca
      421 ccatgaaacc aaatgttgaa ttcgtacgcg aacaagcagg aaagattgtt tatcccgtac
      481 gcaatgtgat gatcatggga ggaagccgta tagctcagaa aactattcag tacctgccgg
      541 ggaatatcaa taccaagatt ctcgaacgag ataaggaaaa atgttatttt ctggccgaaa
      601 aactggagga tacgcttatt atcaatgccg atggacgtaa cgtggatgcc ctcagatcgg
      661 aaggaattca ggaaattgat gcctttgtgg ctaccacagc caattcggaa gcgaatatac
      721 ttgcctgtct cgaagctaaa cgcctgggag tgaagaaaac cgtggcagag gtggagaata
      781 tcgactacat acaactggca gaagatttgg atatcgggtc ggttatcaac aaaaaaatga
      841 tagcagccag ctatatctat cagcttacgc ttgatgccga tgtgctcgat gttcggagtc
      901 tcacatcagc cgatgcagag gtggttgaat ttgtagccaa ggaaggttcc aaaatcacca
      961 aaacacaaat aaaaaacatc cgacttcccg aaaatgtaaa cattggaggt attgtgcgcg
     1021 acggagtggg aagcatcgtt tacggaaata cccagatcat gcctaatgat catgtcatcg
     1081 tattctgtat atcgtcagcc atccgcaagg tggaaacttt atttaattag taaggatatt
     1141 tataattaat ggtttgccga aattgatgtt caccttttac tgccaatgaa cagggattta
     1201 aatatcagat tgattttgaa aatcgtaggt ggactgcttc tggtcgaagc tgtttttctg
     1261 cttttcccat tattagtaac gctcatttac ggcgaagctg aaagtcgatt ttacctgaga
     1321 gcaatcggaa tatctgccgg tttgggcttg gttggtttgt tgtccggaat taaggctgaa
     1381 atgttcttcg gaaagcgcga aggggctgtc attgttacac ttacctggtt gatgttcacc
     1441 cttatcggtg cgctgcctta ctggctgagt ggtaatatac caacctttac cgatgccttc
     1501 ttcgagtctc tatccggttt tacaaccacg ggtacttcca tactgaataa tattgaagct
     1561 ttgccctata acatgctttt ctggcgaagt atgacgcact ggattggcgg tctgggaatt
     1621 atggtcatgt ttctggcgct gatacctttt ctgggaggag ccggttatga gcttttttcg
     1681 tcggaaggaa caacacccat caaagaaaaa attactccaa aaatcagaga aacagccaaa
     1741 tttctgcttc tggtatatat actcctcaca gcactttgta ccatagctct aacattggca
     1801 gggatgagtc tgtttgatgc tgtcaatcat gccttgtcga cgataagtac gggaggtttt
     1861 tcaaccaaac aactcagtat tgcttactgg aattctccgg ccattgaata tgtcatcatt
     1921 ttctttatgt ttttttccgg tataaatttt accatatttt actacatcta caaatcaaac
     1981 tgggatagaa ttaagaaaaa cgaagaactg aaatattaca tccttatatt tctggtgtct
     2041 gttctgatca taatatattc acacatcgac tttagcaagc cattgacttt cgggagcatc
     2101 gaaaaatcgg taaggggaag tctgtttatt gttacttcgt gcataaccac taccggtctc
     2161 tgtaccgaag attacactct gtggagttca tatacctgga tcgttctttt ggtgctgatg
     2221 gttacaggag cttcgaccta ttctacttca ggaggtataa aaatagcacg tgtggtggtg
     2281 ctggctaaat tttgttattc cgaattcaag aaattgattc atcctaatgc tgtcatccct
     2341 gtacgtttca acggatatat cgtgaaggac gacttgctcc tgcgggttct ggccttttca
     2401 attatatatt tactcatcat tgtgacaggt acttttattt taagtctcac tggtctggac
     2461 tttgaagagg caatcagtgg tatgattacc tgtatgagtg atgtgggctt ggggctggga
     2521 aaactcggac cttcatcatc ttttgctgaa atcccagttg tcagtaaatg gtttctggcg
     2581 ttcgttatgc tgttaggtcg tttggaaata tattctgtac ttttactttt tacaccggtt
     2641 ttctggaaaa aataaaactg aaactattat gttcaaatct gaaatcaatt ataaattggt
     2701 gtcgagagtt atcggcaatt tgttgctagt cgaaagtgct gtcatatttt tcgtattgtt
     2761 tattaccctt atttatgggg aaaaagatgg ttggtatttt gttggaacct ctgtgttggc
     2821 attgcttgca ggcttactga tgattgtatt cgggaaaaat gcacccgaca aaatcggaaa
     2881 acgtgaaggc tcaatgattg tcacttttac atggattatc ttttcgctca ttggagcatt
     2941 gcctttctgg cttagtaaaa gtattccttc ctttaccgat gcttttttcg aaacaatttc
     3001 aggttttaca accacaggag cttccattct gaataatata gaagaacttt cacatggaat
     3061 gttgttctgg cgttcgctca ctcactgggt aggaggactt ggaattatcg ttatttcgct
     3121 ggcgctgctt ccggtgttcg gagtgagcgg tacacagctt tttgccgccg aaacaacagg
     3181 tcccacgaaa gataagatcc atcccaagct gaacgaaaca gcaaaacgtt tatttttaat
     3241 atatatcatc cttactgcag ccgaaacaat ctttctgtta tttggacgga tgagcctctt
     3301 cgatgcggtt tgccactctt tttcaaccat agccacagga ggtttctcta ccaagcaggc
     3361 aagtatcgct tattacaatt caccctatat tgaatatgta attactatat tcatgatact
     3421 ctcaggggtc aacttcggca attactattt agcataccaa cggaaatttg ataaaataaa
     3481 agaaaacgag gaattacgct tttatctgat tgtgttgttc ttgtttacgg ttattgttgc
     3541 gctctcactg attgactttt cggaagtggt tactggcagt tctgttgaaa aagcatggcg
     3601 tgaagcattc tttactgttt catcccttat gactactacg ggtatggtta ctgccgatta
     3661 tatgttttgg aagccactta cgtggataat tctaataact gtaatgatga ccggtgcctc
     3721 tgcaggttct acgagtggag gtattaaaat gatccgggta gtcatatcag ccaaagcctg
     3781 ttactatgaa ttcaaaagac tgatacatcc tacagccgtt attccggtta gatacaataa
     3841 acatgtagta cgcgaagata cgcttacgcg ggtaatagcg tttacattgc tttatattgt
     3901 atttgctctg tttgggatac tggttctggc aatttccggt atgggatttc tggagtcaat
     3961 aggaggtatg gttacatgtc tgggtggggt tggtccggga cttggactgg ttggtccgac
     4021 cggtaatttt gctcaaatac ccgaatttag caaatggttt ctcgatttgg tcatgctcac
     4081 cggccgattg gaattatata ccgtcctgat gttgtttacc cctgcatttt ggcgtaaata
     4141 actgatttaa agtgtactta tgttatttga cttgcatttt ctcaattttt cgtgactttt
     4201 acctgtgaaa attctttgaa aaataattgc ccgtttaagt tcttatacct taggtttttg
     4261 aagaaaaaca gcaaatttta tttggaatta tttgcatact gacttgttca ctttaaaaac
     4321 tttgtttacc tttgcacccg ctttgagaga gaagttat
//
LOCUS       DAOPYB010000232         4321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_26418_length_4321_cov_9.462494, whole genome shotgun sequence.
ACCESSION   DAOPYB010000232 DAOPYB010000000
VERSION     DAOPYB010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26418_length_4321_cov_9.462494"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKH58_12185"
     CDS             <1..392
                     /locus_tag="PKH58_12185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNX89832.1"
                     /translation="YLFDSYKNTPINTKMVSLVKKIKKNSKTGLITDNKKDRIDAAKI
                     DFKLIDLFDVISVSAEIGSQKSKESIFSKTVKEFGLSYDECVFIDNQECNLIVPKRLG
                     MKTIFYNHKENNIKRLISELKELSIQI"
     gene            537..1592
                     /locus_tag="PKH58_12190"
     CDS             537..1592
                     /locus_tag="PKH58_12190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNX89833.1"
                     /translation="MNTSKVISRRSNLVLISVLITIIIIVFSTVSFLYVVNDIQKLTN
                     FINSTGSIRGGIQRVTKLYLLNQDIKESVKMIDETNFILSDFVNSKTGVFNSNDKVEI
                     SNLLQSWTEYKEILKNNQKDRILNSSENLWKLSNIVVNKIEIKTHNHIALQYLFLFLL
                     FAIVCILGLVGFFIRKVVQENIEKKASHDQLTNLLNRNYLHQIYNVKLSDSLRKSSFL
                     AVLMCDIDHFKYVNDTFGHDVGDKVLKRIAEIMVENTRENDAIFRYGGEEFLILVSFT
                     EIPQLIQYAERLRSSVESTEIIEHKITISVGVSLIDDKQELKQHILRADTALYKAKQA
                     GRNRVIVNELNLTTAST"
     gene            1723..2172
                     /locus_tag="PKH58_12195"
     CDS             1723..2172
                     /locus_tag="PKH58_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89834.1"
                     /translation="MQMKIGFLLGLLLIGSCSGNTNKQSLRRPLYEHSFAFEKDLPKQ
                     VIDKYGLATPRPPEMCFWDEYAGLTENDLIQRFGRPNNLDTIKIDEINFVGTHTLLAD
                     KKFIIQERGGKIDMAKWTQGEWKLTAYFWPSTKTCLGSVRRHKSVEL"
     gene            2656..3147
                     /locus_tag="PKH58_12200"
     CDS             2656..3147
                     /locus_tag="PKH58_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89835.1"
                     /translation="MSIPKALSHLLLVGGCTGMLLAAQPSSRSATLSLKFVPSGLSPH
                     FEATLANTSKSDIKIYRTLLYGLTIELFDGSGKQIAPRVTGDLCPPAPPNPHESPFVT
                     VHPCQSISVVYDRSIEDLGIEHAGIYYALASYNYTLTPTHKAYPSLTKHPIQSEKIKV
                     TIP"
     gene            3762..4181
                     /locus_tag="PKH58_12205"
     CDS             3762..4181
                     /locus_tag="PKH58_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89836.1"
                     /translation="MGGDIIMFKEDIQEITNNNQPKYTRAILDTFSDPADAVSTYRFL
                     REEQVLLMASHLSDWDRKKYRVCHLFSEFAPVFYYKKRYFSVTGNPFTDMNWKVTVDH
                     SPECEFLPDDWGIDELIDAADRLAHRKKGPRKPPQLD"
BASE COUNT         1268 a          927 c          848 g         1278 t
ORIGIN      
        1 aatatctttt tgattcatat aaaaatacac caattaatac aaagatggtt agtttagtta
       61 agaagataaa gaaaaatagt aaaaccggac taataactga caataaaaaa gatagaatag
      121 atgccgctaa gatagatttc aaactcattg acttatttga tgtaatctct gtttctgctg
      181 aaatcggatc acaaaaaagt aaagaaagta tatttagtaa aactgtaaaa gagtttggtt
      241 tgagttacga tgaatgtgta tttattgata atcaagaatg caacttaatt gtgccaaaaa
      301 gattaggaat gaaaacaata ttttataatc ataaagagaa taacataaaa agattaatat
      361 cggaattgaa ggaattgagc attcaaatat aagacaagaa gctaacaact gcttcaacct
      421 gactcggcta ttgtcacgaa acttgctgtc gcttcgctcg gcaagtttcg cgccaatgta
      481 cgcctcgcag gttaagcatt agttaggttg acgccttcgg cgcagaggag aaaagtatga
      541 acacaagtaa agttatttct cggcgatcta atcttgtttt gatttctgtt ttaatcacga
      601 ttatcataat agtattttca acagtcagtt ttctatatgt agtaaatgat attcaaaaat
      661 taactaattt tattaatagt actggttcaa ttagaggggg aattcaacgt gttaccaaat
      721 tgtatttatt gaatcaggac ataaaagaat cagtaaaaat gatcgatgaa acaaatttta
      781 tattatcaga ttttgtaaat tcgaagaccg gagtgtttaa cagtaatgat aaagttgaaa
      841 tttcaaacct actgcaatct tggacagaat ataaagagat tcttaagaat aatcaaaaag
      901 atcgaatatt aaatagtagc gagaatttat ggaagttatc aaacattgtg gtaaataaaa
      961 ttgaaataaa aactcataac cacattgctt tacagtattt gtttttattt ctattatttg
     1021 cgattgtatg tattttagga cttgttggct tttttatcag aaaagttgtt caagaaaaca
     1081 ttgaaaaaaa ggcttctcac gatcaattaa caaacctatt aaatagaaat tatttacatc
     1141 aaatatataa tgtaaaacta tcagatagtt taaggaaatc aagctttctt gctgttttaa
     1201 tgtgtgatat tgatcatttt aagtatgtca atgatacttt tggacatgat gttggagata
     1261 aggttttaaa aagaatagct gaaataatgg tggaaaatac tcgtgagaat gatgcaattt
     1321 ttagatatgg aggcgaggag ttcttaatac ttgtgtcgtt tacggaaata ccacaattaa
     1381 ttcaatatgc tgagagattg cgtagtagtg tagaaagtac tgaaattata gagcataaaa
     1441 taacgattag cgttggtgta tcattaatcg atgataaaca agaactaaaa caacatatac
     1501 ttcgtgctga cacagcgctg tataaagcaa aacaagcggg ccgcaatcga gtaattgtaa
     1561 atgaattaaa cctaacaact gcttcaacct gatattttct ttgtcacatt ttgtgctgtc
     1621 gctacgctcc ttatgcacaa aatgcgccaa gccctgcggg cgcgaaaata caggttaagc
     1681 aaatgttagg cccgttcatt gttccatttc ctaggattat tcatgcagat gaaaattggg
     1741 tttttgttag gtttgctcct aattggaagc tgttcgggaa acaccaataa acaatccctt
     1801 cggcgaccgt tgtatgagca ttctttcgct tttgaaaaag atctcccaaa gcaagtgatc
     1861 gataagtatg gtcttgcgac tcctcggccg ccggaaatgt gtttttggga cgaatatgca
     1921 gggctaacgg aaaatgatct tattcaaagg tttggcagac cgaataatct cgatactatc
     1981 aaaatagacg aaatcaattt cgttggtact cacactcttc tcgcagacaa aaagtttatc
     2041 atccaagaac gtggtggcaa gattgatatg gcaaagtgga ctcaaggtga atggaaactc
     2101 acggcctact tttggccctc aaccaagaca tgtctcggct cagttcgcag gcataaatct
     2161 gtagaactct aggcctaacc ctcagttcaa ctcggaccca acagcctgtg tcgttttcat
     2221 cctccaatca tcccgatttc cagcctccac tcagcattcg tctggctgtt gtgccggtta
     2281 acatcgttcg ttaggccaat cctctggttt tctcatgaac tcagacaaag ccatcaacaa
     2341 cctccaaacg agaatctctg gcctcgctgc agaaaagctt ggtcggtctt tgacagcaaa
     2401 agaaactgca ttcatcacat ccaggcgcgg ctttcttgca cttgaggcca ttcatgattc
     2461 cgtgtcttcc ttttcatctt cggaagtgga acgttatctc aactccaaat agcttcttac
     2521 atgcggccta acccctcgtt caaccggacc caacagccag tgtcgaatcc ctccttctat
     2581 cgtctcgatt cctcggctcc ttccagcctg cggctggctg ttgggccggt taacagcttt
     2641 cgttaggccg ttccaatgag tattccaaaa gccttatctc acttattgct cgtgggtggc
     2701 tgcacgggca tgctcctggc tgcccaacct tcctctcgtt ctgcaaccct atcgcttaag
     2761 ttcgttcctt cagggttatc tccacatttt gaagcaacac tagccaatac atctaaatcc
     2821 gacatcaaaa tataccgcac tcttttatat ggtcttacca tcgaactttt cgatggatca
     2881 ggcaagcaga tcgctcctag agttactggg gatctatgtc ccccagcgcc tccaaatcct
     2941 catgaaagtc catttgtaac agttcatcct tgtcaatcaa tctctgttgt ttatgaccgc
     3001 agtatcgaag accttggaat tgaacatgca ggaatctatt atgcattagc atcttacaac
     3061 tacacgctta caccaactca taaagcatat ccatcgctta caaaacaccc tattcaatca
     3121 gaaaaaatca aggtcaccat tccgtagcaa cggcctaacc cctcgttcaa ccggacccaa
     3181 cagccagtgt cgaatccctc cttccatcgt ttcgaattct cggccccttc cagcctacgg
     3241 ctggctgttg ggccggttaa cagctttcgt taggccgccc catgaagatc ctttttgatt
     3301 ccctcaaagt taccggaatc gtcctcgtgg ttcttgctgt ctttgttggg ttgagtctca
     3361 tatcgacaag gaattcactt ggctactggt tcgttctatc agccatcttc ctgccggtca
     3421 cgttcttgaa atcctggggc ttccgctggt ggtgtattgg tgccttggtt gcatctgctt
     3481 ctctgacatt tagccccatt gacttttgtg ttcgtcgtgg cgaattcggt ctacgcatac
     3541 ttcctacgag tcgcggaatt gtcagtagac ctggcaccat ttcttatggt tgcgttgcac
     3601 gaaatccgcc caaacatgcc atggtgttat ttctatgaat ccacatctcg tcggcctaac
     3661 ccctcgttca acgcgaaccc aacagccagt ctcctgtctc tactctctcc cacccatcac
     3721 ggcggccagc cacccctggc gatccgaagc atggtataat tatgggtgga gatattatta
     3781 tgtttaaaga agatattcaa gaaatcacaa ataataatca accaaagtac actcgtgcaa
     3841 ttctagatac gttttcagat cctgctgatg ctgtatcaac ctataggttc cttcgagaag
     3901 aacaggtgct tctaatggcg agccatttga gtgattggga cagaaagaag tatcgcgttt
     3961 gccacctttt tagtgaattc gcgcctgttt tctattacaa aaagcggtat ttcagcgtca
     4021 caggtaatcc ttttacagac atgaactgga aagtcactgt tgaccattct ccagaatgtg
     4081 aattcttgcc cgatgattgg ggaattgacg aattgattga tgctgctgat aggttggcac
     4141 acagaaagaa ggggcctcgc aagccccctc agcttgattg agctgatgcc aaagtgatct
     4201 tccagaaacg ccacggttca attcgtggcg ttttctttgt ctggggcctc gggagcgaga
     4261 cagtgtgctt cccaatcctg catcaaggct cttcgcttct ccagcagggt gccacgggca
     4321 t
//
LOCUS       DAOPYB010000233         4308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_26556_length_4308_cov_11.898425, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000233 DAOPYB010000000
VERSION     DAOPYB010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26556_length_4308_cov_11.898425"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PKH58_12210"
     CDS             complement(<1..521)
                     /locus_tag="PKH58_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89837.1"
                     /translation="MLSKSRYTRGCNCQKSLWLYVHKKEEQVIPEATQAIFARGTSAG
                     ELAQNYFPGGKLAVLEDFPGFASAKRTQDFIRQGIETIYEATFIYDNTLVAVDILHKQ
                     DGKWHLYEVKSTNSVKPEHIKDVGVQYYVAKGSGLDIEDAFLMHFNREYVRHGDIEVN
                     KLFIPESILGEVVS"
     gene            complement(531..863)
                     /locus_tag="PKH58_12215"
     CDS             complement(531..863)
                     /locus_tag="PKH58_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89838.1"
                     /translation="MKKIILLTNKNEISKHYNFDEVTDVKNFALAHEMDYQESDDEKL
                     IAKILQVRDKKRSSIETRQYENAAALRDQEWSLVLDHFGCGNEFGRSSSADVGEQYVC
                     VIYRKTKT"
     gene            complement(865..1764)
                     /locus_tag="PKH58_12220"
     CDS             complement(865..1764)
                     /locus_tag="PKH58_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNX89839.1"
                     /translation="MSKRESINRYFLIIKKLRRNPSTFDEIADYLLRESEIQSYDFNT
                     SKRTFQRDLDDIRSLFSIDIQYDFSRKVYYISDESEPEMNIRILEAFDMLNALNLSDG
                     LSKYIHFEKRKPQGTENLNGLLHAIKNCFQIYFSYQKFWDDKPEKRYVEPYALKEFRN
                     RWYLMAKDNKDGKVKSFGLDRLKDLDITRRTFKSPNDYIIEDKYRYCFGIITPDDQEP
                     EEIILSFNAFQGKYIKSLPLHETQQIISDTDKELQIRLKLCITFDFIMELLSLGENMK
                     VLKPKSLQKLIKNRLKATLAQYE"
     gene            complement(1814..3502)
                     /locus_tag="PKH58_12225"
     CDS             complement(1814..3502)
                     /locus_tag="PKH58_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX89840.1"
                     /translation="MKATNKNKLDFNLLQIISRVYKASKNSKVSPVFMKKVSPQLELL
                     SSYFQINKRQAFLLANLYAICCEHRSVEFSDLGRHLGSKPIDLMPLQEDLELLKEKGI
                     LIRKRDSSRGMSGLRYETYQIEESVNSAVIKNLPLPELKNAQCKDIFELLHRIYSNGK
                     DRNDGIISTLELYEETKRILSANKHLSFADKVSNMELGAANTYFLLHMCWKTLSGKPR
                     TDLDELVEMIFDSSFEKARYVQKIISGENELVSNKWIELEECFFVNDMEVTLGKKGVL
                     LLEEENIKLFLKDTVRDNVINPESIGEKTLFFGGSEQQQLKMLEEMLLDKNLLPMQER
                     LKVKSLPQGIAVLLYGAPGTGKTESVYQFARKTGRQIMHVDISESKSMWFGGSEKKIK
                     QIFTDYNEFFNKTERIPILLFNEADAILSKRKDSNFSNVSQTENAMQNIILEEMEKFK
                     GILFATSNLTGNMDSAFERRFLFKVEFHKPDNKIREKIWKSKFEYIPETGCAELAKRF
                     DFSGGQIGNIVRKCEMFEILNGTLPDIGKICEFCDEELINKECRNSIGFKINNN"
     gene            3648..>4308
                     /locus_tag="PKH58_12230"
     CDS             3648..>4308
                     /locus_tag="PKH58_12230"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNX89841.1"
                     /translation="MKSTKHYKDVLAINVGSANTTFVYKGKVVLDEPSLISMNRITGK
                     YEDVGSKVLIKQQQAAENIEILGSSPHGFFPSYSIYKTLVEKFYRIIRSKISDLPATP
                     DIIFSVPYNISGFQKDILRESAIEIGAQNVWFIYKPLAIAVGCEIDFIRPEAIMIVDI
                     GACFNEISVIALGGVINELSGHIAGNEFDNDIKNFLLLRYNLIPNLEKVYVLYSVKLI
                     PV"
BASE COUNT         1206 a          850 c          787 g         1465 t
ORIGIN      
        1 gagacaacct cgcctagaat gctttcaggg ataaagagtt tgtttacctc aatatcgcca
       61 tgacgaacat actcacgatt aaaatgcatc agaaaagcat cttctatatc aagcccgctg
      121 cctttggcta cataatactg aaccccgaca tctttaatat gttctggttt gacactgttg
      181 gtgcttttca cttcgtacaa atgccatttt ccgtcctgtt tatgaagtat gtcaaccgct
      241 accaacgtat tatcataaat gaatgtagct tcgtaaatag tttcaatgcc ctgccttatg
      301 aagtcctgag tccttttggc tgaagcaaaa ccggggaagt cctctaatac ggcaagtttg
      361 ccgcccggaa aatagttttg agctaattca ccggcactgg taccacgggc aaaaatggcc
      421 tgtgtagctt cggggatcac ttgttcttct tttttatgga cataaagcca taatgatttc
      481 tggcagttgc agccgcgagt atatctggat tttgagagca taattattat ttaagttttt
      541 gtttttctgt aaatcacaca cacatattgc tcacccacat cagcagatga actacgcccg
      601 aattcattac cacagccaaa atgatccaat actaagctcc attcctggtc acgcaaagca
      661 gctgcatttt cgtactggcg ggtctctatg ctggaccttt ttttatccct gacttgaaga
      721 attttagcga taagtttttc atcatccgat tcctgataat ccatttcatg cgcgagtgca
      781 aaattcttca catcagtgac ctcatcgaag ttataatgtt tcgatatttc atttttgttt
      841 gtcagcagta tgattttttt catgttactc gtattgtgcc aaagttgcct ttagccggtt
      901 cttaataagt ttctgcaaac tttttggctt cagtactttc atattttctc caagtgaaag
      961 caattccatg atgaaatcaa atgtaatgca caactttaat cttatctgca attctttatc
     1021 tgtatctgaa atgatctgtt gtgtttcatg cagaggcaat gatttgatgt atttaccctg
     1081 aaaagcatta aatgataata taatttcttc gggttcctga tcgtcgggag tgatgatacc
     1141 aaagcaatat ctatacttat cttcaataat gtaatcgttc ggggatttaa aggttcttct
     1201 ggttatgtcg aggtctttga ggcgatcgag gccgaagctc tttacctttc catctttatt
     1261 gtctttagcc atcagatacc agcggtttct gaattccttg agagcatagg gttctacata
     1321 cctcttttcg ggtttatcat cccagaattt ctggtaagaa aagtaaatct gaaagcagtt
     1381 ttttatggca tgaagcaatc cattcagatt ttcggtgccc tgtggttttc gcttttcaaa
     1441 gtgaatgtat tttgacaagc catcggatag attcaaggca ttgagcatat caaacgcctc
     1501 gagaatacgg atattcattt ccggctcgct ttcgtcactg atgtaataaa ccttacgtga
     1561 aaagtcgtat tgaatgtcaa tgctgaacaa agagcggatg tcgtcaaggt cgcgctggaa
     1621 tgttcgcttt gaagtgttaa aatcatagga ttgaatttct gattcacgta aaaggtagtc
     1681 ggcaatctca tcaaaagtgg atggatttct cctgagtttt tttattatca ggaagtaccg
     1741 gttgatagat tctctttttg acataggttt atttttcatc aaatataatt ataatgctac
     1801 gacaaaatat ggcttagttg ttatttattt taaaacctat ggaatttcga cattctttat
     1861 tgatcagttc ctcatcacag aattcgcata ttttgccaat atctggtaaa gtgccattca
     1921 aaatttcaaa catctcacat tttctgacga tattaccaat ttgcccgcct gaaaaatcaa
     1981 atctctttgc aagctcagca cagccggttt ccgggatata ctcgaatttt gatttccaga
     2041 ttttttccct gattttatta tccggtttat gaaattccac cttgaataaa aaccgccgtt
     2101 cgaaggcgct atccatattg ccggtaaggt tgctggtggc aaataaaatc cctttaaact
     2161 tctccatttc ttccagtata atattttgca tggcattctc cgtttgcgac acattagaga
     2221 aattgctgtc tttgcgtttg gacaagatag catcggcttc gttgaacagc aaaatcggga
     2281 tacgttctgt tttattgaaa aattcattat aatccgtaaa aatctgtttt atttttttct
     2341 cgcttccgcc aaaccacatg gattttgatt cgctgatatc tacgtgcatg atctgcctgc
     2401 cggtttttcg ggcaaactga taaacagatt cggtttttcc tgtaccaggt gcgccataca
     2461 acaatacggc aattccctgt ggcagagatt tcactttcag gcgttcctgc atgggcagca
     2521 ggtttttatc cagaagcatt tcttccagca tcttcagttg ctgctgctcg ctgccgccaa
     2581 agaacaatgt tttttcccct atggattccg gatttatcac attatccctg acagtgtctt
     2641 ttaaaaaaag tttgatattt tcttcttcaa gcaggagaac acctttttta cccagcgtca
     2701 cctccatatc gttaacaaaa aaacattctt ccagttctat ccatttattg ctaaccagtt
     2761 cattttcacc agatataatt ttctgcacat acctggcttt ttcaaaacta ctgtcaaaaa
     2821 tcatttcgac caactcatcc agatcggtgc ggggcttgcc cgatagagtc ttccagcaca
     2881 tatgcaataa aaaatatgta tttgcagcgc ccaactccat attacttacc ttatctgcaa
     2941 aactcagatg cttatttgca gacagtattc tttttgtttc ttcgtatagt tcgagggtcg
     3001 aaataatacc gtcatttcta tccttgccgt tggaatatat tctgtgcaat aattcaaaaa
     3061 tgtctttgca ttgtgcattt ttcagttcgg gtagcggcaa gttttttatt acagctgaat
     3121 tgaccgattc ttcaatctgg taggtttcat acctcaggcc agacattccc cttgagctat
     3181 cccttttcct tatcaaaatg cctttttctt ttaataattc aaggtcttcc tgcagaggca
     3241 tcagatctat aggtttgctt cccaaatgcc tgccaagatc gctgaattct acgctacgat
     3301 gctcacaaca aatagcatac agattggcta acaaaaatgc ctgccgcttg tttatctgaa
     3361 agtatgacga cagcagttcg agttgagggc ttaccttttt catgaagact gggctgactt
     3421 tactgttttt cgatgctttg taaactctgc tgattatttg cagaagatta aaatcaagct
     3481 tgtttttgtt tgttgctttc atgatttatt atcttttgga agcaaaataa agaagggagt
     3541 acgacaaagg gtgtcggggg taaaaataaa cacagaaaat ttttattttt cataccacgt
     3601 cacagaatgg cataccagta ttttaatttt gcaaaaaatt aaggattatg aaaagtacaa
     3661 agcattataa agacgtttta gcaattaatg ttggctctgc taatacaaca tttgtttata
     3721 aaggcaaagt tgttctggat gaaccatcat tgatttcaat gaatcgtatt accggtaaat
     3781 atgaagatgt aggttctaaa gtactgataa agcaacagca agcagcagaa aatattgaga
     3841 ttttagggtc ttcaccacat ggattttttc ctagctatag catatataag actttagttg
     3901 aaaagtttta tagaattatc agatcaaaaa tatccgattt acctgccaca ccggatataa
     3961 tttttagtgt accatataat attagtggtt ttcaaaagga tatattgagg gagtcagcaa
     4021 tagagattgg ggctcagaat gtttggttta tatataagcc attggctatt gccgtaggat
     4081 gcgaaataga ttttatcaga cctgaagcca ttatgattgt tgatattggc gcttgtttta
     4141 acgagatcag cgttattgcc cttggtggcg taattaatga attatccggg catattgccg
     4201 ggaatgaatt tgataatgac attaaaaatt tcctactctt acgctataat ttgatcccta
     4261 acttggaaaa agtttacgta ttatactctg taaaattgat cccggtcg
//
LOCUS       DAOPYB010000234         4263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_26986_length_4263_cov_8.225760, whole genome shotgun sequence.
ACCESSION   DAOPYB010000234 DAOPYB010000000
VERSION     DAOPYB010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26986_length_4263_cov_8.225760"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2962
                     /gene="mfd"
                     /locus_tag="PKH58_12235"
     CDS             <1..2962
                     /gene="mfd"
                     /locus_tag="PKH58_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNX89842.1"
                     /translation="PEAILQKQITSQGLKTKTIRLRTGESIGMDFLIEMLVEYGFERV
                     DFVYEPGQYSVRGGIVDIFSYAYEMPYRIDFFGDEVESIRIFDIETQLSQEKRQQVEI
                     VPNLRKGDKTTKLVPITEFVAADTWFQFTDAGFVRDRINQLYDEALIKANSGDHVVES
                     LHTTHITGDEFIQGVDKFRKLEWANKSYFKTKTTIQFNTSPQPVFQKNFDLISENLKT
                     NLTEGYRVYIFSDSVKQTDRIAAIFADREDNITFQPVKNTLHEGYIDHDLSLVCYTDH
                     QIFDRFHKYQLKTDKTRLGKVVMTLKELNQFQVGDYMVHVDHGVGKFGGLVRTNVNGK
                     MQEMVKLIYKDDDIIFVSIHSLHRISKYKGKEGEAPRVNKLGSGVWERLKERTKTKVK
                     DIARELIKLYAKRKAEAGYQYSPDSYMQQELEASFIYEDTPDQSKATADIKKDMESEM
                     PMDRLVCGDVGFGKTEVAIRAAFKAVADSKQVAVLVPTTVLAMQHYNTFKDRLKDFPC
                     TISYLSRAKAPKQSKETLDRLEKGEIDIIIGTHKLVGKTVKFKDLGLLIIDEEQKFGV
                     TVKERLKELKVNVDTLTLTATPIPRTLQFSLLGARDLSIINTPPPNRYPVQTEIHAFD
                     EDVIREAIQLEMNRNGQVFFVNNRIQNIYMIEAMIKRLVPGCRVAVGHGQMPAEKLEE
                     VIVDFIDYEYDVLVATTIIESGIDIPNVNTIIINSAHRFGLSDLHQLRGRVGRSNRRA
                     YCYLLAPEMSLLTPDARRRLQAIETFSELGSGFNIAMQDLDIRGAGNMLGAEQSGFIA
                     DLGYETYQRILNEAVHELKDEEFAELYADERVDKDRSKAYVTDCQIDTDLELMFSSEY
                     IENVSERINMYRELDNLENENELNAFQLQLEDRFGKLPQQAIDLMLIVRLRWNAMRFG
                     VEKLVLKNGQMIAYLVSNLKSTYYQSDEFGRLLQYMARNPRICRLREQNGKRSVIFTD
                     INNIQKAFHIFEGI"
     gene            3199..>4263
                     /locus_tag="PKH58_12240"
     CDS             3199..>4263
                     /locus_tag="PKH58_12240"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HNX89843.1"
                     /translation="MKKLLYYNSSLTLFLISLISGAQTVASQTVLVTNKSDKTLRDYT
                     VEVPLSGSRLTAGNYVVVSSENEIVPLEISTDLKGDQKAIFPVADIKPFSTTKYYLKK
                     GNADKYPKRTYAEIAHKIGGKFAGKVYEGGFSWVKPNYMRMPDNFTDHAYYIKYEGPG
                     WESDKVAFRFYLDWRNGIDVFGKRTPGIVLPFVGTEDYEKYHHMADWGMDNMKVGNSL
                     GLGSIAVWNGTKAVRVEKTDSVISLIPADGKIRSQVLTNYYGWQANGVKCNLKSLISI
                     DAGSRASRMELSVDKNLPNIATGIVKDKNAELIKPEKSTGEWSYIATFGKQSLNNDMQ
                     GLTVFYRTKQLQKITEDELSH"
BASE COUNT         1353 a          838 c          958 g         1114 t
ORIGIN      
        1 tccagaggca attctgcaaa agcaaatcac ttcacagggt ctgaaaacca aaaccatcag
       61 actgagaacc ggtgagagca tcggtatgga ctttctgatt gaaatgttgg ttgagtatgg
      121 atttgagcgt gtggactttg tttatgaacc gggacaatac tcggtgaggg gcggaatagt
      181 tgatattttc tcgtatgcct atgaaatgcc ttatcgtatc gacttcttcg gtgatgaggt
      241 ggaatccatc cggattttcg atatcgaaac acagctttcg caggaaaaaa gacagcaggt
      301 agagattgtg cctaacctga ggaaaggtga taaaaccact aaactcgtac ctataactga
      361 atttgttgcg gcagatacct ggtttcagtt taccgacgcc ggctttgttc gcgatcgtat
      421 caatcaactt tacgatgagg cactcataaa agccaatagt ggcgatcatg tggttgagag
      481 cctgcataca acacatatta caggggatga atttatacag ggtgtagata aatttcgtaa
      541 actggaatgg gccaataagt catattttaa gactaaaacg acgatacagt tcaacacgtc
      601 accacagcct gtatttcaaa agaattttga tctgatcagc gaaaacctga aaacgaatct
      661 gacagaaggc tatcgcgttt atattttcag cgacagcgtc aagcaaaccg accgtatagc
      721 tgccatattt gccgacagag aagacaatat cacatttcaa ccggtaaaaa acaccctgca
      781 tgaaggttat atcgatcatg acttgtcgct cgtatgttat accgaccacc agatatttga
      841 ccgttttcat aagtatcagt taaaaaccga taagaccaga ctgggaaaag tggtgatgac
      901 tctcaaggaa ctcaaccagt ttcaggtggg cgactacatg gtgcatgtgg atcacggtgt
      961 tggaaaattc ggtggactgg taaggacaaa tgtgaacggt aagatgcagg aaatggtaaa
     1021 actgatttat aaagatgatg atattatttt tgtaagtatt cattcactgc accggatatc
     1081 caaatacaaa ggaaaagaag gtgaagcacc acgcgtcaac aaactgggca gtggcgtttg
     1141 ggaacggctg aaagaacgta ccaaaactaa agtaaaagac atagcccgcg agcttataaa
     1201 actctatgcc aaacgtaaag ccgaagccgg ataccaatat tcacccgaca gctatatgca
     1261 gcaagagttg gaagcttctt ttatatacga agatacccct gatcagtcaa aagcaaccgc
     1321 tgatataaag aaagacatgg agtccgaaat gcccatggac cggcttgttt gcggcgatgt
     1381 gggatttggg aaaaccgaag tggcaatccg tgctgctttc aaagctgttg ccgacagcaa
     1441 gcaagtggca gtgcttgtgc ccaccactgt gcttgccatg cagcattaca ataccttcaa
     1501 agaccgatta aaagattttc cgtgcaccat ttcctacctt agcagggcta aagctccaaa
     1561 acaatcaaag gagacgcttg accgattgga aaaaggggaa atagacataa ttatcggaac
     1621 ccacaaactt gtagggaaaa cggtaaaatt caaagacctc ggcttgctta tcatcgatga
     1681 ggaacaaaag tttggagtga ccgtaaaaga acgcctgaaa gaactgaaag tcaatgtaga
     1741 tacgctcaca ctgaccgcta caccaattcc ccgtacactg caattctctt tgcttggtgc
     1801 acgtgacttg tcgattatca acactccacc accaaaccgt tatccggtac aaaccgaaat
     1861 acatgcgttc gatgaagatg tgataaggga ggccatacag ttagaaatga accgaaatgg
     1921 tcaggtattc tttgttaaca acagaataca gaacatctat atgattgagg caatgattaa
     1981 acggttggtg cctggttgtc gggtagctgt gggtcatgga caaatgccgg ctgaaaaact
     2041 tgaagaagtg atcgtggatt ttattgatta tgaatatgat gtgctggttg ccacaactat
     2101 catcgaatcc ggtatcgaca ttcctaatgt aaataccatc atcattaata gcgctcatcg
     2161 gtttggactg agcgatttac atcagctccg gggcagggta gggcgaagca atcgcagagc
     2221 ttactgttac ctgcttgcgc cggaaatgag tttgctcact cccgatgcca gacgcaggtt
     2281 gcaggctatt gaaactttct ccgagctggg aagcggattt aatatcgcca tgcaggattt
     2341 agatatacgt ggggccggaa atatgcttgg cgccgaacaa agcggattta ttgccgatct
     2401 gggttatgaa acctatcagc ggatactcaa cgaggcggta catgagctaa aggacgaaga
     2461 atttgcagaa ctatatgctg acgaacgggt tgataaggac agaagtaagg cttatgttac
     2521 cgattgtcag attgatacag atcttgaact gatgttttcg tccgagtata tcgaaaatgt
     2581 gtcagaacgt atcaatatgt accgcgaact cgataatctg gaaaatgaga atgagttgaa
     2641 tgcctttcaa ctgcagcttg aagaccgttt cggcaaatta ccccaacagg caattgatct
     2701 catgctgatt gtcagattga gatggaatgc aatgcgtttc ggtgttgaaa aactggtatt
     2761 gaaaaacgga cagatgattg cttacttagt atccaacctg aaatcgacat attaccagtc
     2821 cgacgaattt ggtcggttat tgcaatatat ggcccgcaat ccgcgaattt gccgtcttcg
     2881 cgaacaaaac ggcaagcgct cggttatttt taccgatatc aataacatac agaaagcctt
     2941 ccatatattt gagggtatct aataaatgct gcagatttac tgctgtctca aaaataaaat
     3001 cagaataata atgtaagacg gaatggtttt tttgccgtga tatatgtatt ttatcattat
     3061 tatatacttt attatcatat ttttggtact tttttatact gttttgctga agccagcata
     3121 aaaattatga ttatatttgc acggacttaa tctcccgaaa aacgaaattc tattgtcaat
     3181 tttttatcag tattcaagat gaaaaaatta ctttattata acagcagtct aacgctgttt
     3241 ttaatttctc taatatctgg tgcgcaaacg gtcgcttcac agacggttct ggtaacaaat
     3301 aaatcggaca aaacactccg ggattatacc gtggaagtgc cactgtccgg ttctcgactt
     3361 acggcaggaa attatgtggt tgtttcgtct gaaaatgaaa ttgttccgct cgaaatttca
     3421 accgacctca agggagatca gaaggcaatt tttcctgtag ccgatatcaa accattttcc
     3481 acaacaaaat attatttaaa aaaaggaaac gccgacaaat atcccaaacg tacgtatgct
     3541 gaaattgccc acaaaattgg tggaaaattt gccggaaagg tatatgaagg tggattctcg
     3601 tgggtaaaac ccaattacat gcgtatgcct gacaacttta ccgatcacgc ctactatata
     3661 aaatacgaag gtccggggtg ggaaagtgac aaggtagctt ttcgctttta tcttgactgg
     3721 cgtaacggaa ttgatgtatt tggaaaacgc acgccgggta ttgtgcttcc gtttgtaggg
     3781 actgaagatt acgaaaaata tcaccatatg gcagattggg gaatggacaa tatgaaagtg
     3841 ggaaactccc tgggtttagg ttcaatagcc gtttggaatg gaactaaggc tgtacgtgtt
     3901 gaaaaaactg acagtgtaat cagccttata cctgccgatg ggaaaatacg ttcgcaggta
     3961 ttgacaaact attacggatg gcaggcaaac ggagttaaat gcaacctgaa atcgctcata
     4021 tcaattgatg ccggcagtcg tgcttcgcgc atggagcttt cagtggataa aaacctgcca
     4081 aatattgcca ccggtattgt caaagataaa aatgcagaac tgataaaacc cgaaaaatct
     4141 accggggaat ggtcctacat agctactttt ggcaaacaaa gtctcaacaa cgatatgcaa
     4201 ggacttaccg tattttaccg cacaaaacag ttgcaaaaaa ttactgaaga tgaattaagc
     4261 cat
//
LOCUS       DAOPYB010000235         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_27171_length_4242_cov_8.650346, whole genome shotgun sequence.
ACCESSION   DAOPYB010000235 DAOPYB010000000
VERSION     DAOPYB010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27171_length_4242_cov_8.650346"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PKH58_12245"
     CDS             <1..1143
                     /locus_tag="PKH58_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX89844.1"
                     /translation="LTGFKNYPKALFYLEKSFSLSQKINDSTRSIQLENIALAYMGMK
                     KYSAALQHLRMSLNLSEQFKSKLGIASNCCNIANMYLKINKPDEAKQYLDRSLTISRE
                     INSQGLIRVVYNLYTLYYAIKGDVDKMWDYADKSDEITDTLYNDEGKKLIADMQTKYE
                     TEKKEQEIKLLSVENKLKESQLRIRTFLLFGLAIALTFIFIYIFQKRSLLFAQKNLVQ
                     KNLEIVESENKLNILKENSNFNKVPQNDKQEDHSSKYTSSLLSEEQKIQIKELISCYF
                     NSSKNFLHQDYSIYDLANDLKINRNYISQVINEKLHKNFSSLLNEYRIKEARKILSES
                     SNLKFTIESIAESVGYKSKTTFNNAFKKFVGVTPSFYINSIKDSLG"
     gene            1377..3791
                     /locus_tag="PKH58_12250"
     CDS             1377..3791
                     /locus_tag="PKH58_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89845.1"
                     /translation="MKKKLLFLTVCGLLFSTAVSAQTTYVTKESNPANNRITNNRNIP
                     TMPEKLNFQMFDKTERVTPLSEGLWNSNGPYGGLVFSFAIDTTNNILYAGTRNGVWRS
                     ENGGESWEYSLIPETGYITWGWNMVNSISVLKTNPDIVYAICATGLYKSTDRGLSWNQ
                     VLTPGVFGSVKAFAYNANEFVCVSVYGTGIEKSNDGGQSWENITPHNLSPSGPIIVNE
                     DNPDIILAGANGSGIFKTVTGDTNWVQLNNGIPTDLTTVALTRNPKNHNTLYACLSKI
                     FPDQLGIPGLYKSYNAGESWQLINSDFDFHKSLYEFDMMDINPIDTNMVFIAYDDSLY
                     KSYDGGITFNNSSSPLIAGCITVNFDPANTEVIYLGKYIGISKSNDGGTIWSESSHGM
                     LGVTPTKVAINKDNQNILYVVSNGLNGLDKSMDGGLTWQNSNTGLPGYLSVTLDPMNQ
                     GTLYCVTPFDGIYKSADSGNSWGQLTDQPIYTYEIDHSNSNNQYGSIDNIFYKSYDGG
                     ATWNEISTITTGGVFDGAIVSISKDPNNQDVFYCGTQPFWSTDMFGGLFKSTDGGITW
                     TQLVADQAPVRIAIDPNNSDIVYYGTCENGLFKSTDGGLSWNSLTIGLGTGESLPYIS
                     DIIINTENSNIIYVGLQNNFYNSSFVVNNGIYRSTDAGLTWEAMPMEGIPWKDVENLS
                     LSPDNNRLYASIQCGGVFFYDILSGIEEPDSKIMTMLHIYPNPVNSSTKIFFELSTNS
                     KVTLRLFDVNGRCITDLCDQDFVKGKHSCPLNLTACNGEKISPGVYYVKLTTNYSTET
                     AKVVIK"
BASE COUNT         1420 a          791 c          791 g         1240 t
ORIGIN      
        1 ttaacaggtt ttaaaaatta tcccaaagct ttgttctatt tggaaaaatc gttctcatta
       61 tcgcaaaaaa taaatgattc cactcgctcc atacaattgg agaacattgc tctggcctat
      121 atgggaatga aaaagtacag cgctgctttg cagcatctca ggatgtcatt gaatctctct
      181 gaacaattta aatcaaaact tggcatagca agtaattgtt gcaatatcgc caatatgtac
      241 ctgaaaataa ataaaccgga tgaagccaaa caatatcttg accgttcgct gaccatctca
      301 agggagatca attcacaagg gcttataagg gtagtatata atttatatac cctctattat
      361 gctattaaag gcgacgttga taaaatgtgg gattatgctg ataaaagtga tgagataacg
      421 gatactctgt ataatgacga aggcaaaaag ctcattgctg atatgcaaac caaatatgaa
      481 acagaaaaga aagaacagga aatcaaattg ttgagtgtgg agaacaaatt aaaggaatca
      541 cagcttagaa tccgcacttt cttattattc ggattagcta ttgctctgac attcatcttc
      601 atttatattt ttcagaaacg cagtttgtta tttgcacaaa aaaatctggt ccaaaaaaat
      661 ctagagatcg tcgaatctga aaacaagttg aatattctga aagaaaattc caattttaat
      721 aaggttccac aaaatgataa acaggaagac cattcctcaa aatatacaag ttcactgttg
      781 tctgaagaac aaaaaattca gattaaagag ttgatttcat gctatttcaa tagcagtaaa
      841 aactttctgc atcaggatta ttccatttat gatcttgcga atgacctgaa aattaacaga
      901 aattatatct cgcaggtgat caatgagaaa ctccataaaa atttcagcag tttgctgaat
      961 gaatatcgaa tcaaagaagc ccgtaaaata ttatccgaat cttctaattt aaaattcact
     1021 attgagtcta tcgccgaatc agtagggtac aaatcaaaga cgactttcaa taatgccttt
     1081 aaaaagtttg tgggtgtcac cccctctttc tacatcaatt caataaaaga ttctctggga
     1141 taataacaaa atattgttct gtcattgtgt tgattacctg aacattttgg catttaccca
     1201 tttcggcctc accgttaatt ctgttcagat gtgtacatgt ggacatggat gcaatcctct
     1261 gtcttttttt gtatcatact tttgcaatgt tctcgcctgc ttttagaagt tttaatggct
     1321 atggtaaaaa cccttgtgta aagaagatct gatatcaaac taaaacctca ttcattatga
     1381 aaaaaaaact actatttctg acagtttgtg gtctgctgtt ctcgacagcg gtttcagccc
     1441 agactaccta tgttaccaaa gagtctaatc cagcaaacaa tcgaataact aataatcgta
     1501 atatcccaac gatgccggag aaacttaatt ttcagatgtt tgacaaaaca gagcgggtga
     1561 cccctttaag cgaagggctc tggaactcaa atggcccata cggaggactg gttttttcat
     1621 ttgccatcga tacaacaaat aatatacttt atgccggaac cagaaacggg gtatggagaa
     1681 gtgaaaatgg aggcgagagt tgggaatact ctctaatccc cgaaacgggg tatataacat
     1741 ggggctggaa tatggttaac tccatatctg tgttaaaaac caaccctgat attgtatatg
     1801 ctatatgcgc gactggcttg tataaatcaa ccgatagagg gctctcctgg aatcaggttt
     1861 taacaccagg cgtatttggc agtgttaagg catttgcata taatgccaat gaattcgtgt
     1921 gtgtttcggt atacggcact ggcatcgaga aaagcaacga tggcggtcaa agttgggaaa
     1981 atattactcc tcacaactta tcgccttcgg gtcccatcat tgtaaatgaa gacaaccctg
     2041 atattatttt agccggagcc aatggttcag gaatttttaa aacagtgact ggcgatacaa
     2101 actgggtcca actgaataat ggaataccaa cagatctgac tacagtggca ttaacacgaa
     2161 atccaaagaa ccataatacc ttatatgcat gcctgagtaa aatattcccg gatcagcttg
     2221 gaataccggg cttatataaa tcgtataatg ccggggaaag ctggcaactt ataaattcgg
     2281 attttgattt ccacaaaagc ttgtatgaat ttgatatgat ggatataaac cccatcgata
     2341 ccaacatggt attcattgca tacgatgata gtttgtataa aagttacgac ggtggaatta
     2401 ctttcaataa ttcttcttca ccattaatag ctggctgtat tactgtaaat ttcgatccgg
     2461 caaacactga agttatttac cttggcaagt atattgggat ttcaaaaagt aatgatggag
     2521 ggactatctg gagtgaaagc agccatggga tgcttggagt aactccgaca aaggtggcta
     2581 ttaataagga caatcaaaat attctatatg ttgtgagcaa cggattaaac ggtttggata
     2641 aaagtatgga cggtggatta acctggcaaa attccaacac cggactcccc ggataccttt
     2701 ctgtaacact tgatcccatg aaccagggaa ccctgtattg tgttactcca ttcgatggta
     2761 tttataaaag cgctgattcg gggaattcat ggggacagct tactgatcaa ccaatatata
     2821 cctatgaaat tgatcattca aacagcaaca atcaatacgg atccatcgac aatattttct
     2881 ataaatcata cgacggagga gccacctgga atgaaatttc tacaattacc acaggtggcg
     2941 tttttgatgg tgcaattgta agcatttcaa aggatccaaa caaccaggat gttttttatt
     3001 gcggaacaca gccattctgg agcaccgata tgttcggtgg attgtttaaa agtacagatg
     3061 gcggaatcac ctggacacag ttagtagcag atcaggctcc tgtgagaata gccattgatc
     3121 ctaataattc agatatcgtt tactatggta cttgcgagaa tggacttttc aaaagtactg
     3181 acggcggtct ttcctggaac tcattgacca tcggtttagg cacaggggaa agtctccctt
     3241 atatcagcga cattataata aatactgaaa acagtaatat tatttatgtc ggacttcaga
     3301 ataatttcta taactcatca ttcgtagtaa ataatgggat ttacaggagt acagatgcag
     3361 gattaacatg ggaagcaatg ccaatggaag ggataccttg gaaagacgtg gaaaaccttt
     3421 cgctttcacc agataacaac aggctgtatg cttcaattca atgcggcgga gtgtttttct
     3481 acgatatttt aagtggtatt gaagaacctg attcaaaaat tatgaccatg ctccatattt
     3541 acccaaatcc tgttaattca agtacaaaaa ttttcttcga actttcaacc aattcaaaag
     3601 taacactccg gctctttgat gtaaatggcc gttgtataac cgatctctgt gaccaagact
     3661 ttgtaaaggg aaaacattca tgcccattga acttaaccgc atgtaatggt gaaaaaattt
     3721 caccgggtgt gtattatgta aaattaacca caaactattc gaccgaaaca gccaaggtgg
     3781 taataaaata aaacaacacc ggacctatac attttagtaa aaccagagat aaaaaaagtg
     3841 ttcactctct atcaattggg tcaaccctgg ggtaattctt tgggttgacc caattaatat
     3901 ttttcatgac tccactttaa gaaagaaatc agatttttga atataactac tccaaaagaa
     3961 tcctccggtt tagagaatcg taataaagac cttgaaaaat actttctaag tttatgagag
     4021 gaagtatgta cacaacatta tacggacttt tgccctgatt tacttagttt ttcaatttac
     4081 tcatgtctat ttgttttctc taattcaact catcagtttc tgcattccca gtccaaatta
     4141 attttaataa aacctggccg gcaattcagg ggatcaaaag aatcccgacc acgtcatatc
     4201 ctgtcgttat caccaaatac ctttgtcaaa aaaaagatga ac
//
LOCUS       DAOPYB010000236         4211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_27453_length_4211_cov_7.168672, whole genome shotgun sequence.
ACCESSION   DAOPYB010000236 DAOPYB010000000
VERSION     DAOPYB010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27453_length_4211_cov_7.168672"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    146..245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(440..1804)
                     /locus_tag="PKH58_12255"
     CDS             complement(440..1804)
                     /locus_tag="PKH58_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNX89846.1"
                     /translation="MKSFHKYFPAFISLTTVTLPVAGQKHTDTRPNIIFILADDMGYG
                     DLACYGNKIIQTPNIDKLAAEGIRFTQAYAGSAVSSPSRCALLTGKHTGHGTVRDNFA
                     KKGGLTGLKNGNTIRRMHLLPEDTTIATILSAVGYKTCIVNKWHLDGFNPNAGPLDRG
                     FDEFYGWLVSKDESNLPYYYPALRYYNRELIEISENRNNAGGIHNSDLSVRESIGFIE
                     RNRKNPFFLYLAFDVPHEPYFINDLEPYGSMPLSETAKLYASLITHTDKAIGNLIEYL
                     EKNNLRDNTLIIFASDNGGAAQAPLEELNCNAGLRGRKALLYEGGIKVPFIVNYPGKI
                     KPGQIKDDLIYFPDVMPTLAAFSGAKLPHNVDGISILPLLQGKRQNTGKRILYWEFPG
                     QQVAIRKGKWKAVSVNKSSELELYDISKDPFEKNNLASRYPKIVNELKREIEKSRVAS
                     PYYE"
     gene            complement(1874..3103)
                     /locus_tag="PKH58_12260"
     CDS             complement(1874..3103)
                     /locus_tag="PKH58_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNX89847.1"
                     /translation="MKTPQILFFLLLIIASAVSAQSKTDEEAVRAIADNILKQPVTQF
                     VGVNDNKTYNSTQEIPPGTDVKFKSPLSEWHYSNGVLDMAMITLGKYLNEPTYVNYAK
                     NHVAFGFENYSYFKNTFRNDRKHWHWPFGQLWNMKELDDCGAMGAAVIEVYQLDERNE
                     YREYIDKAANHIMNVQDRLKDGTLCRKFPKEMTVWADDAYMATSFLTQMGKMTSDKKY
                     FVEAARQLINIDKYLWCPEKQLYYHCYYTGLKRNGVAFWGRANGWIMVSLADLIEVLP
                     VNHPKRAKLIALLEKQIVGASRYQNANGMWNQLLDKSDSYDESSVTAMFVYAIAKAVN
                     NKWIDPTYASLAKTGWNVLKNKEITSDGRFTNVCVGTGITDDIPFYYNRPVGDNEKHG
                     LGLIIDTAVEMMKMSKK"
     gene            complement(3116..>4211)
                     /locus_tag="PKH58_12265"
     CDS             complement(3116..>4211)
                     /locus_tag="PKH58_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HNX89848.1"
                     /translation="QNGKDAFFFSVDLPEGNYRVKLTLGNPEFAANTTVRSESRRLML
                     ENIKTPKGKFITKEISVNIRNTKIGSTDSVRIKARENGKLNWDNKLTLEINGVNPSLA
                     RLEITSAENIPTIFLAGNSTVVDQDNEPWCGWGQILPRFLNSKIAVANYAESGEAGNT
                     FIRSRRFGKILHEMKKGDYLFIEFGHNDMKQKGENAGAFKSYKQSLVTMIDETRKKGG
                     TPILVTPMHRRRFDDNGKVINTLDDFPDAVRLTGKELNVMVVDLNAMSKVLYEAWGPE
                     ESIRAFVHYPAGTFPGQKTDLADNTHFNSFGGYEICKCILKGLIDNKSPLKKYIVKDY
                     KSFNPAKPDKFNQFNIPATPFSSIEKPDGN"
BASE COUNT         1033 a          873 c          849 g         1356 t
ORIGIN      
        1 caaatgtgga aatcgtcagg aacaaaaaag ctaaaattaa tgggagtttt ttcatgtgtt
       61 tataatttat ttagcggccc cctaatccgt cagctgacgg aggaatattg agcgtctctc
      121 atttgatgtg cgttatttaa tcttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnccatt gtgtgtccat ttttttagtt ggacatacgt gcttcatgtg attgtttttt
      301 atttagcggc cccctaaccc cctaaagggg ggatattgag cgtctctcat ttgatgtgcg
      361 ttatttaatc ttgccattgt gtgtccattt ttttagttgg acatacgtgc ttcatgtgat
      421 tgtttttttt gctataaact tattcgtaat acggtgatgc cacgcgtgat ttttcaattt
      481 ccctcttcag ttcattcaca attttaggat accgcgaagc caggttattt ttctcaaaag
      541 gatctttgga aatatcataa agctctaatt ctgagctttt attcaccgaa acggctttcc
      601 attttccttt gcgtatggct acctgctgtc ccggaaattc ccagtacaaa atccgtttgc
      661 ctgtattttg tctttttcct tgcagtaaag gcagaatact tattccatcg acattatgcg
      721 gaagttttgc tccggaaaat gctgccagag taggcattac atccggaaaa taaatcaaat
      781 cgtcttttat ttgaccgggt tttattttcc cgggataatt cacaataaat ggcactttaa
      841 ttccgccttc gtaaagcaac gcttttcgtc ctctcaaacc tgcattgcag tttaattctt
      901 ccagcggcgc ctgagcagca ccgccattat cagaagcaaa aataatcagc gtattgtccc
      961 gtaagttgtt tttttccaaa tactcaatca gatttccaat ggctttatcg gtatgggtaa
     1021 ttaacgaggc atatagtttt gcggtttcgg aaagcggcat ggatccgtaa ggttccaggt
     1081 cgttgataaa atagggttca tgcggcacat caaaagccag gtaaagaaaa aacggatttt
     1141 tcctgtttcg ttcaataaaa ccgatggatt ctctgacaga caaatcgctg ttgtgaatgc
     1201 ctcctgcgtt attcctgttt tcagagattt ctattagttc tctgttgtaa tatctcagtg
     1261 ccggataata atacggaaga tttgattcat ctttactcac cagccatccg taaaattcat
     1321 caaatcccct gtcgagcggt ccggcattgg gattaaatcc atccagatgc catttattga
     1381 caatacaggt tttatatcct acggccgata gtatggttgc aatggtcgta tcttcgggca
     1441 gcaggtgcat cctgcggatt gtattaccgt ttttcagtcc ggtcaatccc cctttttttg
     1501 caaaattatc gcgaacagtg ccatgtccgg tgtgttttcc ggtaaggagt gcgcatcggg
     1561 agggtgaact gacggccgaa ccggcataag cctgcgtgaa tcggattcct tcggcagcca
     1621 gtttgtctat attgggagtt tggataattt tatttccata acaggctaaa tcaccataac
     1681 ccatatcgtc ggccaaaata aagatgatat ttggacgtgt atcggtatgc ttttgtccgg
     1741 caacgggaag cgtgacagtt gtcagactga tgaaagccgg aaaatattta tggaacgatt
     1801 tcatggcaaa agattatgat ttgaaaaatt gtggtctttt ggtgaagtcc ttaatggaat
     1861 gcttaaatag aaattatttc ttactcattt tcatcatttc caccgctgta tcaatgatta
     1921 agccaagtcc gtgtttctcg ttatcaccta ccggtcgatt ataatagaag ggaatatcat
     1981 ccgtaatccc tgttccgaca catacatttg taaaacgtcc atccgaagtt atttccttat
     2041 ttttcagcac attccatcct gttttagcta agcttgcata cgtggggtca atccatttat
     2101 tgtttaccgc tttggcaatt gcatatacaa acatggcggt aactgatgat tcgtcgtacg
     2161 agtcactttt atcgagcaac tggttccaca ttccgtttgc attctggtat ctggatgccc
     2221 caacaatttg tttttcgagc aacgctatta attttgcccg ttttggatgg ttgacaggca
     2281 ggacttcaat caggtcggcc agcgaaacca taatccagcc gttggcacgt ccccagaaag
     2341 ccactccgtt acgtttcaat ccggtataat aacaatggta gtaaagctgt ttttcgggac
     2401 accacaggta tttgtcgata ttgatcaatt gtcgggctgc ttcaacgaaa tactttttat
     2461 cactggtcat tttacccatt tgagtgagaa aagatgttgc catatacgca tcatctgccc
     2521 atacggtcat ttctttcggg aatttccggc aaagagtccc gtccttcaac cggtcctgca
     2581 cgttcattat gtgattggct gccttatcga tatattcccg atattcgttt ctctcgtcga
     2641 gctgatacac ttcaatcaca gctgcaccca tagccccgca gtcatccagt tccttcatgt
     2701 tccaaagctg cccgaatggc cagtgccagt gtttgcggtc attccggaat gtatttttga
     2761 aataggaata attttcaaat ccgaatgcta catgattttt ggcataattg acatacgttg
     2821 gttcattcag gtatttgcca agggtaatca tagccatatc gagcactccg ttggaataat
     2881 gccattccga gaggggagat ttaaatttca catctgtacc cggtgggatt tcctgtgtac
     2941 tgttataggt tttattatca ttgacaccca caaactgggt aaccggttgc tttaaaatat
     3001 tatcggcaat ggctctgacg gcctcttcgt cggtttttga ctgcgccgac acagcacttg
     3061 cgatgattaa taacagaaaa aataaaattt gaggtgtttt catgattttc gggatttaat
     3121 ttccatccgg tttttctatc gaactaaaag gagtagccgg gatattaaac tggttaaatt
     3181 tatcgggttt tgcaggatta aaagacttat aatctttcac aatgtatttt ttcaacggcg
     3241 atttattgtc aatcaaccct ttcaaaatac atttacatat ttcgtagcct ccaaaactgt
     3301 tgaagtgtgt attgtcggcg aggtcggttt tttgtccggg aaatgtgccg gcaggataat
     3361 gaacaaaggc cctgatagat tcttcgggtc cccaggcttc gtacagtact ttgctcatgg
     3421 cattcaggtc aactaccatc acgttcaatt cttttccggt caaacgtact gcatcgggaa
     3481 aatcgtcgag cgtgttaatg acttttccat tgtcatcgaa gcgccgacgg tgcatgggag
     3541 ttacgagtat aggagtgcct cctttttttc gggtttcatc tatcatggtg accaaactct
     3601 gtttgtagct tttgaatgct ccggcatttt cacccttttg tttcatgtcg ttgtgtccga
     3661 attcgataaa aaggtaatcg ccttttttca tttcatgcag tatttttcca aatcgccggg
     3721 aacggataaa ggtattccct gcctcaccgg attcggcgta gttggcaact gctatttttg
     3781 aattcaggaa gcggggcaag atctgtcccc aaccacacca gggctcgttg tcctgatcca
     3841 caacagtaga atttccggcc agaaaaatcg tgggaatatt ttccgcagat gtaatttcaa
     3901 ggcgagccag agatggattg acaccattga tctcgagtgt cagtttatta tcccaattta
     3961 gttttccatt ttcacgggct ttaattctta ctgaatcggt actgccgatt ttagtgttgc
     4021 gaatgttgac tgaaatttct ttggtgataa actttccttt cggagttttg atattttcaa
     4081 gcatcagtct tcttgattct gaccgaacgg ttgtgtttgc agcaaattcc ggatttccca
     4141 gtgtcagttt tactctgtaa ttcccttccg gcaaatcgac agaaaagaaa aaagcatctt
     4201 tcccgttttg a
//
LOCUS       DAOPYB010000237         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_27898_length_4161_cov_7.220653, whole genome shotgun sequence.
ACCESSION   DAOPYB010000237 DAOPYB010000000
VERSION     DAOPYB010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27898_length_4161_cov_7.220653"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..270)
                     /locus_tag="PKH58_12270"
     tRNA            complement(196..270)
                     /locus_tag="PKH58_12270"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(233..235),aa:Ile,seq:cat)
     gene            complement(567..1748)
                     /locus_tag="PKH58_12275"
     CDS             complement(567..1748)
                     /locus_tag="PKH58_12275"
                     /inference="COORDINATES: protein motif:HMM:NF016364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNX89849.1"
                     /translation="MKRIRFILLLLLTMQTVFAQKSSVNEFAAIDKRALQLPDSLTKT
                     TDQIATYIKTNFSTDKDKSRAIFIWIASNIQYDIDNMFAINFYEKREEKIAKPLRTRK
                     GVCENYASLFTDICNRSGIKSFVIEGYTKQNGFTDYIPHAWSAALIDSSWFLFDPTWG
                     SGYVDGRKFFKKINNDYYKARPTTLIKSHMPFDYLWQFLNYPVTNQEFYEGKTQENKT
                     KPFFDFIDTLNVYEKQNHIDQLISSAYRVEKNGVKNSLVFDRLQHIKLEIENDRQTKI
                     INLYNSSVTDYNDGITAFNDFINYKNKQFTPKKTDTEIQGMIDIAENKFKEAKSKLVQ
                     ISNADQNTTYMTRQLIKSIDDASKQMKEQQDWLKLYFSKSKTVRKSMFYKVTWFGIPL
                     N"
     gene            complement(2045..3802)
                     /locus_tag="PKH58_12280"
     CDS             complement(2045..3802)
                     /locus_tag="PKH58_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX89850.1"
                     /translation="MKKTIIVLLSLSYTLCFAQTKNSTLPDKLPQIPDISKLQKENSF
                     PMGNLDSMAEIKLYDLPNASGPYEPTWESIEKNYPGEPAWLRDAKFGIWVHFGPQAAG
                     ESGDWYARRLYQNNTKAYENHQKKYGHPSVNGYKEVLRDWNPKKLDPSALTKIYQDAG
                     ARYLVIQGVHHDNFDLWNSHYQPWNSVNLGPKRDLIGEWAKACRAADMHFGITFHHEY
                     TWWWWQTAFGCDTIGDKKGIPYDGNLTLKDGKGKWWEGLDPRLLYGVNLREYKGVIDA
                     ANSKWSPPPAGIFKNHLPYAKWYATQWALRMMDAVDQYDPDFIYTDGTDQQPFSGSGT
                     GTGVKADAMQRVISYYYNRTLERRGEVNTFSIVKFRHKTNGTVNTEEFGIPANIKKDQ
                     PWIGEIPVGDWFYAPDFTYNSGMMIRYVIEAIARDGNAAISIALLPDGSLDESSQKML
                     QEVGDWMRINGEAVYGSRAWVIPGEGELVNGKLKMLPGGNLSRRHAEFKFGNQDFRFT
                     TGKNDALYAFCLTVPSPGSNLKIKSLGTEADYLSHPVKTVKLLGYDGKLEWQQQVDGL
                     SITCPEAMPFETSVVFRIE"
BASE COUNT         1153 a          833 c          775 g         1400 t
ORIGIN      
        1 catcgaatgt atattgagca aataaaccag attggcaaat gtttgaagat tcgttctcac
       61 cattgtattg tatagacaat gggcgtccgg ctaaacgatt cggctaatgg cagaattgtt
      121 gagagtaagg catttagcaa cggcataaaa aaagcacctg tagaacgtgt ctgcaagtgc
      181 ttgattttta aattgtgggc ccagttgggc ttgaaccaac gaccccctga ttatgagtca
      241 ggtgctgcta accaactgag ctatgggccc tcttttccgt aaatggagtg caaaaataac
      301 atattttcca gtaagaaaaa aataaaatga gaaaaaatat tcctccgacc ggcaaatttc
      361 aattaattaa cgtgaggtga gatatttgaa ttttgaagtt tcacgtttca ggtttcacgg
      421 agttgaaagt tcaacttata taattcctgc tgtatatatt caacgtgtac atgatgaata
      481 tttatttcca atgtcattaa gcgttttcag gctttttaca ttcaggcagg tggctggagc
      541 acaaagtttc aatttcatac tcataatcag tttagtggaa tgccaaacca cgttacttta
      601 taaaacatag attttcgtac agtcttactt ttactgaagt acaactttaa ccaatcctgt
      661 tgttctttca tttgttttga agcatcgtca atagatttga ttaattgtct agtcatataa
      721 gttgtgttct ggtctgcgtt tgatatttga accaacttgc ttttggcttc cttaaattta
      781 ttttctgcaa tgtcaatcat accttgtatt tcggtgtctg ttttcttcgg agtaaattgt
      841 ttgtttttgt aatttataaa gtcgttaaaa gcagttattc cgtcattata gtcagtcact
      901 gaagagttgt aaagattaat tatcttggtt tgtctgtcgt tttctatctc taattttata
      961 tgttgaagcc tgtcaaacac taacgagttt ttcacaccat ttttctctac acgatatgct
     1021 gatgatatta gttggtctat gtggttttgt ttttcataaa cattaagtgt gtcaatgaaa
     1081 tcgaaaaacg gtttagtttt attttcttgt gttttacctt cgtaaaattc ctggtttgtt
     1141 acaggataat taaggaattg ccacaaatag tcaaatggca tgtgagattt gataagtgtt
     1201 gtcggtctag ctttataata gtcgttattt atttttttga aaaactttcg accatcaaca
     1261 tagcctgaac cccaggttgg gtcaaagaga aaccatgaac tatctataag cgccgcactc
     1321 caggcgtgag gaatatagtc tgtaaatccg ttttgctttg tgtaaccttc aattacaaaa
     1381 gatttaatac ctgatctatt acaaatgtct gtgaacaacg atgcataatt ttcacaaacg
     1441 ccttttcttg tcctcaatgg ttttgctatt ttttcttctc tcttttcgta aaaatttatt
     1501 gcaaacatgt tgtcgatgtc atattgaatg tttgatgcaa tccaaataaa aatagctcgt
     1561 gatttatcct tgtcagttga aaagttggtc ttaatgtatg ttgcaatttg gtccgtggtc
     1621 ttggtcaaag agtcgggcaa ttgtaatgct cttttgtcaa tcgctgcaaa ttcattcaca
     1681 gacgactttt gagcaaaaac agtctgcatc gttaagagta gaagcaatat aaatcttatt
     1741 cgtttcattt tgatgtagta gtatggatgc ctataatggc aacttgtagc cgttgtttgc
     1801 ccttttaccg cgttattgtt ctggtgtaac attggaaaga caaatggata ctattcccca
     1861 tctatcttat tattttcagt tcatctaaat tgtcaattat caatttgaat acgactatta
     1921 gctcaatcat ttcatcttta cttccctcat ttctttgaat tatacataaa aagctggaaa
     1981 tttacctcat ccacttgtta gattcaaaca ttataatcac actcaattca tactttgaat
     2041 gcttttattc tattctgaat acaactgatg tttcaaatgg cattgcttcc ggacaggtaa
     2101 ttgaaagtcc gtcaacttgc tgttgccatt ccagttttcc gtcatatcca agcaatttta
     2161 cagtttttac cggatgactc aaataatctg cttcagtacc taatgactta atttttaaat
     2221 tggatccggg ggatggtaca gttaggcaaa acgcgtacag cgcgtcattt ttacctgttg
     2281 tgaaacggaa gtcctgattg ccgaatttga actcagcatg acgacgactc aggtttccac
     2341 ccgggagcat ttttaatttt ccattaacca gttcaccttc gcccggaatg acccatgcac
     2401 gacttccata aactgcttct ccatttatgc gcatccagtc acctacttcc tgtagcatct
     2461 tttgggaact ttcatccagt gaaccatccg gaagaagggc aatactgatt gcggcattac
     2521 catcacgggc tatcgcttcg attacatatc gaatcatcat tccagaattg tatgtaaagt
     2581 ccggagcgta aaaccagtct cctaccggta tttcccctat ccatggctga tcctttttga
     2641 tatttgccgg aataccaaat tcctctgtgt tgactgtacc attcgtcttg tgacggaatt
     2701 tcacgatact gaaggtattg acctctccac gacgttcgag tgtccggttg taataatagg
     2761 aaataactcg ttgcatggca tcagccttaa caccggttcc tgttccactt ccactaaaag
     2821 gttgctgatc ggtaccatcg gtatatataa aatccgggtc atactgatcc accgcgtcca
     2881 tcattcgcaa tgcccattgg gtggcatacc atttagcata aggcaggtga tttttaaaaa
     2941 tgcctgcggg tggcggagac cattttgaat tggctgcatc aatcacccct ttatattcac
     3001 gcaggttgac accataaagc agacggggat caagcccctc ccaccatttt cctttgccat
     3061 ctttcaacgt taagtttcca tcataaggaa ttcccttttt atctcctatg gtatcgcagc
     3121 caaatgcagt ttgccaccac caccaggtat attcatgatg aaacgtgata ccgaaatgca
     3181 tgtctgctgc acggcaagct tttgcccatt ctccgattaa atcgcgtttg gggccgagat
     3241 taacagagtt ccatggttga taatgagaat tccataaatc gaaattatca tgatgaacgc
     3301 cctgaatgac aagatatcgt gctcctgcat cctgataaat ctttgtaagc gcgctcgggt
     3361 ccagtttttt tgggttccaa tcacgcaaga cttccttgta tccgttcaca gatggatgac
     3421 cgtatttctt ttgatggttt tcgtatgctt tcgtattatt ctgataaagc ctgcgggcat
     3481 accagtcgcc actttcgcca gctgcctgcg ggccgaaatg aacccagatg ccaaactttg
     3541 catcgcgtag ccaggctggt tcaccgggat aatttttctc aatggattcc caggttggtt
     3601 cgtacgggcc ggatgcatta gggagatcat acagcttaat ctcagccata gaatccagat
     3661 tacccatggg aaaactgttt tctttctgta gctttgagat gtctggtatt tgaggaagtt
     3721 tatccggcaa agtggaattc tttgtctgcg cgaaacacaa cgtataagag agacttaata
     3781 atacaataat tgtttttttc attttcggaa tttattctga tttacccggc taaaatacat
     3841 cttttttcta aataatatta ttttgattgc aatgcctgtc aaattctgct taagattatt
     3901 ttctatctaa aatcccgaat aaacacaagt caaaattaaa tgataaacat agtagaatat
     3961 gtgtcttttc ttttttactc acgttcggct taccttccag actataggcg taaacctaaa
     4021 gatcatagca aatatagtga tatgttatgg catttaggtt tacgcctatg acgaaaacgc
     4081 tactccaaat agtgtggccg ataaatgcaa acaaaggggt acgggctgat ggtgttagct
     4141 tacacagcaa ccaacgcact t
//
LOCUS       DAOPYB010000238         4110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_28406_length_4110_cov_11.762022, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000238 DAOPYB010000000
VERSION     DAOPYB010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28406_length_4110_cov_11.762022"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1299
                     /gene="tuf"
                     /locus_tag="PKH58_12285"
     CDS             112..1299
                     /gene="tuf"
                     /locus_tag="PKH58_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNX89851.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHIDHGKTTLTAAITLVLSQKGWSEF
                     RSFDSIDNAPEEKERGITINTAHIEYSTANRHYAHVDCPGHADYIKNMVTGAAQMDGA
                     ILVVAATDGPMPQTNEHILLARQVGVPTIVVFMNKVDLVDDEELLEIVEMEIRDRLTF
                     YGFDGEKTPIIRGSALGGLNLEPKWVEKIVELMDACDAYIPLPPRETDKDFLMPVEDV
                     FSITGRGTVATGRIETGVINSGDPVEIIGLGAEKLKSVVTGVEMFRKILDRGEAGDNT
                     GLLLRGIDKDQICRGMVVAKPGSVKPHTHFKAQVYILKKEEGGRHTPFHNKYRPQFYF
                     RTTDVTGEITLPEGIEMVMPGDNLVIDVKLISEIALTLNLHFAIREGGRTVGAGQVTE
                     IIE"
     gene            1376..1448
                     /locus_tag="PKH58_12290"
     tRNA            1376..1448
                     /locus_tag="PKH58_12290"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1409..1411,aa:Trp,seq:cca)
     gene            1465..1656
                     /gene="secE"
                     /locus_tag="PKH58_12295"
     CDS             1465..1656
                     /gene="secE"
                     /locus_tag="PKH58_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNX89852.1"
                     /translation="MKKIKNYIKESYDELVHKVSWPTWSELQNSAIVVSIASVIIAIV
                     VYLMDISFSTLLKQFYTLF"
     gene            1719..2261
                     /gene="nusG"
                     /locus_tag="PKH58_12300"
     CDS             1719..2261
                     /gene="nusG"
                     /locus_tag="PKH58_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNX89853.1"
                     /translation="MSDQVKKWYVVKAISGKEKKVKELIESEINHQNLQDFVSQVLIP
                     TEKVYQVRKGKKISKERNFFPGYVLIEAVLVGEVAHIIKNVTGVLGFLGSRGEPIPMR
                     TAEVNRILGKVDELSEKGEEISEPFIIGESVRVIDGPFNSFTGIIEEVNEEKKKLKVM
                     VKIFGRKTPLELSFMQVEKE"
     gene            2297..2740
                     /gene="rplK"
                     /locus_tag="PKH58_12305"
     CDS             2297..2740
                     /gene="rplK"
                     /locus_tag="PKH58_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNX89854.1"
                     /translation="MAKEVSGLIKLQIKGGAANPSPPVGPALGAKGVNIMEFCKQFNA
                     RTQDSVGKLIPAIITVYKDKTFDFIIKTPPVAVQLLEAIKKTKGSAEPNRNKISSISW
                     DQVKQIAEGKMVDMNAFTIESAMKMVAGTARSMGISIQGNPPFTI"
     gene            2770..3462
                     /gene="rplA"
                     /locus_tag="PKH58_12310"
     CDS             2770..3462
                     /gene="rplA"
                     /locus_tag="PKH58_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNX89855.1"
                     /translation="MAKITKKRKEIIKLIDKNAVLSLEEAAVLVKKITTTKFDSSVDI
                     DVRLGVDPRKADQMVRGIVTLPHGTGKTIRVLVLCTPDKEEEAKNAGADFYGLDDYIT
                     KIKEGWTDVDVIITMPSVMPKVGALGRVLGPRGLMPNPKSGTVTMDVGKAVKEVKAGK
                     IDFKVDKFGIIHAGIAKVSFDSDKIAENAKEILQTILRLKPSSAKGTYIKSIYMSSTM
                     SPGIQIDTKSVV"
     gene            3493..4020
                     /gene="rplJ"
                     /locus_tag="PKH58_12315"
     CDS             3493..4020
                     /gene="rplJ"
                     /locus_tag="PKH58_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNX89856.1"
                     /translation="MKKEEKDKVIDALSDTLKSVSNFYVTDISDLTVAKTNDLRRLCF
                     KRNIKLTVVKNTLLKKAMERSGRNFESLYPVLVGGTSLMVCETANVPAKLITEFRKAN
                     KMEKPILKGAFIQESTYIGNDQLTALVALKSKNELIGDIIGLLQSPAKNVISALQSGG
                     HTISGIVKTLSEKPE"
BASE COUNT         1386 a          763 c          872 g         1089 t
ORIGIN      
        1 agcacttcct tggtaaggaa ggggtcatga gttcaattct catcaatggc tcaacacaga
       61 ataataatta aacagaaaat aaattataat cacttaacaa acttaattaa aatggcaaaa
      121 gaaaaattcg atcgttccaa accccatgtt aacataggga caataggtca catcgaccat
      181 ggcaagacta ccttaacggc agctatcact ttggtactgt cacaaaaagg ctggtcagaa
      241 ttcagatcat ttgattcaat cgacaatgct cctgaagaaa aagaaagagg tattaccatc
      301 aataccgctc acattgagta ttcaacagct aacaggcact atgctcacgt tgactgcccg
      361 ggacatgccg actacattaa aaacatggtt acaggtgctg cccagatgga cggtgcaatc
      421 cttgttgtag cagctactga cggcccgatg cctcagacca acgaacacat tttgctggct
      481 cgccaggtag gtgttcctac gattgttgtt tttatgaaca aagttgacct cgttgatgac
      541 gaagaacttc tcgaaatcgt tgaaatggaa atcagagaca gactgacatt ctacggattt
      601 gacggcgaaa aaactcctat tatcaggggc tctgcattag gcggattgaa ccttgaacct
      661 aaatgggtgg aaaaaatcgt tgaactgatg gatgcatgtg atgcttatat tcctctgcct
      721 ccccgcgaaa ctgataagga tttccttatg ccggttgaag acgtattttc aattacaggc
      781 cgtggaacag tcgctaccgg aagaattgaa accggtgtca tcaattcagg agatcctgtg
      841 gaaattatcg gactcggcgc tgaaaaactg aaatcagtgg ttaccggtgt tgaaatgttc
      901 cgcaagatcc ttgacagagg cgaagccggt gacaatacag gtctgttgct cagaggtatt
      961 gataaagatc agatttgccg tggtatggtt gttgcaaaac cgggttcagt aaaaccccac
     1021 acccacttca aagctcaggt ttatatcctg aaaaaagaag aaggtggacg ccacacaccg
     1081 ttccataaca aataccgtcc ccagttttat ttcaggacta ccgacgttac cggagaaatc
     1141 accttgccag aaggtatcga aatggtaatg cctggcgata acctggtcat cgacgttaag
     1201 ttgatttccg agatcgcatt aacactgaac ctccattttg ctatccgcga aggtggcaga
     1261 acagttggtg ccggacaggt aactgaaatt attgaataat aaattacgat aacttaggtg
     1321 ttccggttct tccggaatac ctatagttat tataaagctt gttatgtgac tataaacggg
     1381 tgtagctcaa ttggtagagt ggcggtctcc aaaaccgtag gctgtgggtt cgactcctac
     1441 cacccgtgca aataaaagct ttttatgaaa aaaataaaaa attatattaa agaaagttac
     1501 gacgaattgg tacataaagt ttcatggccc acatggtcag agcttcaaaa tagtgcaatt
     1561 gttgtgtcta tcgcttccgt tattatcgct attgtggtat atctgatgga catatcattc
     1621 agtaccttac tgaaacagtt ttatacactg ttttagtatt attatcaaaa gcgttgttct
     1681 gtcaatttca tttcgtatct cccaatggag tttaatttat gagcgatcaa gtaaaaaagt
     1741 ggtatgtagt taaagccatc agtggcaaag aaaagaaggt taaggaattg attgaaagtg
     1801 agatcaatca ccagaacctt caggactttg tttctcaggt acttatacct acagaaaaag
     1861 tttaccaggt aagaaaaggt aaaaagatca gtaaggaaag aaatttcttt cctggttacg
     1921 tactgatcga agccgtacta gttggtgaag tagctcatat tattaagaat gttaccggcg
     1981 tattgggctt cctgggttca agaggtgaac ctattccaat gagaaccgcc gaagtgaaca
     2041 gaatattagg aaaagtggat gaactctctg aaaaaggaga agaaatcagc gaacccttta
     2101 tcattgggga atctgtaaga gtaattgacg gaccttttaa cagttttacc ggtatcatcg
     2161 aagaagtgaa cgaggaaaag aaaaaactaa aagtgatggt aaagatcttc ggaagaaaaa
     2221 caccgctgga actgagcttt atgcaggtcg aaaaagaata acggtttatt gttttattat
     2281 gctaaaataa agaaaaatgg ctaaagaagt aagcggatta atcaaattac aaatcaaggg
     2341 tggggcagca aatccttcac caccggttgg acctgcatta ggtgcaaaag gtgtgaatat
     2401 tatggaattt tgcaaacagt ttaacgcacg tacgcaagac agcgtcggga aacttatccc
     2461 tgcgatcatt actgtttaca aagataaaac ctttgatttt atcattaaaa ctcctccagt
     2521 agcagtacaa ttactcgaag caatcaaaaa aacaaaaggc tcagccgaac ccaaccgaaa
     2581 taaaatcagc tcaataagct gggatcaggt gaagcagatt gctgaaggaa aaatggttga
     2641 tatgaatgca ttcaccatcg aatcagcaat gaaaatggtt gctggtactg cacgcagtat
     2701 gggaatatct attcagggaa atccaccttt tacaatataa taagtggtaa actttttaac
     2761 atttgaacaa tggctaaaat tacaaagaaa agaaaagaga tcataaagct cattgataaa
     2821 aacgctgtat tatctttaga agaagctgct gtattggtga aaaaaatcac cacgacaaaa
     2881 ttcgactctt ctgttgatat tgatgtgcgt ttgggtgtcg acccccgtaa agccgaccag
     2941 atggtacgcg gtattgttac cctgcctcat ggtactggaa aaacgatcag ggtcttggtt
     3001 ttatgtactc ctgacaaaga agaagaagct aaaaatgccg gagcagattt ttacgggctc
     3061 gatgactata tcaccaaaat taaagaagga tggaccgacg tagatgttat tattactatg
     3121 ccaagtgtca tgcctaaagt tggtgcttta ggccgtgtcc taggtccgag aggattaatg
     3181 cccaacccta aatcaggtac ggtaaccatg gatgtcggga aagctgtaaa agaagtaaag
     3241 gccggcaaaa ttgattttaa ggttgataaa ttcggtatca tccatgctgg aattgccaag
     3301 gtttcgttcg acagcgataa aatcgctgaa aacgctaagg aaatcctgca gaccatatta
     3361 cggttgaaac cgtcatcggc taaaggtact tatattaaaa gcatttacat gtcttctaca
     3421 atgagccccg gtatccagat cgacacaaaa tcagttgtat aatttattta acaaggaaat
     3481 aatctgaaat atatgaaaaa agaagaaaaa gataaagtta ttgatgcttt gagtgatacg
     3541 ctgaagtctg tcagtaactt ttacgttacg gatatttccg atttgactgt tgcaaaaacg
     3601 aacgatctgc gtcgtttatg ttttaaacgc aacattaagc tcaccgttgt gaaaaataca
     3661 ctgcttaaaa aagcaatgga gcgttcggga cggaattttg aatcactata ccccgtactg
     3721 gtaggcggca cttctttaat ggtatgcgaa accgccaacg tgcccgcaaa attgatcact
     3781 gaattcagga aagccaataa gatggaaaaa ccaattttaa aaggcgcttt tattcaggaa
     3841 tcgacttata taggcaacga tcagctaacg gcacttgtag cattaaaatc taaaaatgag
     3901 cttatcggag acatcatcgg actactgcaa tcaccggcta aaaacgttat cagcgcttta
     3961 cagtctggtg ggcatacgat ttcaggtatc gtaaaaacat tatcagaaaa acctgagtaa
     4021 aacaaataat tagtaatcta ttgtaaacca aattaaaaac ttaaataaaa tggcagattt
     4081 aaaggctttt gcagaacaat tagttaattt
//
LOCUS       DAOPYB010000239         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_29605_length_3999_cov_8.939148, whole genome shotgun sequence.
ACCESSION   DAOPYB010000239 DAOPYB010000000
VERSION     DAOPYB010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29605_length_3999_cov_8.939148"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PKH58_12320"
     CDS             <1..931
                     /locus_tag="PKH58_12320"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HNX89857.1"
                     /translation="LLKMAVIIMTLVMTTSLSASRKLCLIGDATRYGWDKGNASPMIQ
                     DPTNPSVFYYNAWLKTGNFKFILENTDDSWLPTWNKADDTHIVKRASGSDPDNQFSIL
                     TDGNYSITVDTTTLTISIVPMTETAEISFNTVFMVGDATPNGWDIANATELTKSSTNP
                     YEFSYSGAMSIGEFKLPVNRNSGWGQDFFMKFSDTQMALQKSPDSKWNITETGNYTID
                     INTQTLAISIQKQTTTTSRFPNDESITLKSTIVSSTLKVNNLNSVSYKIYNLSGLKVT
                     DGISEGENINVFSLGKGIYLLNVNNQVFKFIKQ"
     gene            1012..>3999
                     /locus_tag="PKH58_12325"
     CDS             1012..>3999
                     /locus_tag="PKH58_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX89858.1"
                     /translation="MNKILNILLSFLLITFLTKAQSSFLAEQGVAVFCPADMDSVRTL
                     PSLALVKNLQPQGSVPDNWKTRPVFSKINGKSAVTITFDEHVDLYGNGEVTGTLKRNN
                     TKVTLWNTDNYTYSAKDNGTRLYQSHPWVLGVRKDGSSFGIIADNSWKQYFDLSNPIT
                     ITSEGPGFRVIVIEKGNPMEVIKTLSELTGKMNLPPLWALGYQQCRFSYYPASRVKEV
                     ADEFRNRKIPCDVIWMDIDYMDNYKIFTFDPAKFPDPKGLNDYLHSKKIKSVYMIDPG
                     VKKETGYSVYEDGKFGNHWVIDKNGKEFNGSVWPGACAFPDYTNPVTQSWWAGLYKDF
                     MDKGVDGVWNDMNEPSVFDGPDGTMPEDNIHRGGSGLPQDIHCRYHNVYGMLMVKSSR
                     EGIMAANPDKRPFILSRSNFLGGQRYAATWTGDNRSTEEHMKLSIPMSLNLSLSGQPF
                     SGPDVGGFDGDGNADLLGKWMALGAYFPFYRNHSSSNTVNQEPWAFGTKIESVSRTAI
                     NRRYLLLPYLYTLFREASVDGTPIMQPAFFADFQDTTLRSEEQIFLLGKDLLVVPRWA
                     SNINFPKGDWDKIKFENFDDGYQATVLLREGAIVPMGTLIQSTEDYNTDSVTLLINPA
                     SDGTAAGIMYDDASNGYGYKSGDYDIQQFSASKYKEDSLKITINKIEGSRKSTRLFRI
                     GYVTGNDVVYSDWSSNTTQYIKYIPDNLMTVDHSLFPSMFIGGSFNKWNPASMPMQYQ
                     GNGKWKSTRVNLLAGQHEFKFISLKNRTGVEWGNAAGTMGTAGIISNDTIGINFTLSE
                     NGEYIISFNESTLNYSILKAPKYDYLSIVGDATPADWTPSGIALIQDSTNLNIFSYKG
                     QLKEGNFKFHAVNGDWCDGDWLLATSQDQTLTSTEYTVFTGCPSDIQDLKWKVSTAGS
                     YNITINLEARTISIKSLDYFPNLFLAGSATAGGWDLRYTSDMNANPLNPANFSWAGKL
                     SPGEFKIGTTKTWGNGWPWIHPKT"
BASE COUNT         1310 a          690 c          802 g         1197 t
ORIGIN      
        1 tttacttaaa atggcagtca ttataatgac tcttgtgatg acaacttcac tttctgcaag
       61 tcggaaattg tgtttaattg gtgatgcaac ccgttacggt tgggataaag gtaatgcgtc
      121 gccaatgatt caggacccga ccaatccatc agtattttac tacaatgcct ggcttaaaac
      181 aggtaatttt aagtttatac tggaaaatac cgatgactca tggctaccaa catggaataa
      241 agcagatgac actcacattg taaaacgtgc ttctggtagc gatccagata accaatttag
      301 tattttaact gatggaaatt attcaattac tgtagatact actactctta ctatttcaat
      361 tgtaccaatg acagaaacag cagaaattag ctttaatact gtatttatgg taggagatgc
      421 aacgcccaat ggttgggata tcgctaatgc aacagaattg acgaaaagct caaccaatcc
      481 ttatgaattt agttattcag gggcaatgag cattggtgaa tttaaattac ctgttaatag
      541 aaattctgga tggggtcagg attttttcat gaaattttca gatactcaaa tggcacttca
      601 aaagtcacct gattctaaat ggaacataac tgaaactgga aattacacta tagatatcaa
      661 tacgcagaca ttagctattt ccattcagaa acaaaccaca acaacaagtc gattccctaa
      721 cgacgaatcc attacattaa aatcaaccat agtaagttca acattaaaag tgaataatct
      781 aaattcagtt agctataaaa tctataattt gtctggtctt aaggttactg atggaatctc
      841 tgaaggagaa aatattaatg tgttttcttt aggaaaagga atttatctct taaatgtcaa
      901 taatcaggtg tttaaattca ttaaacaata attgtatttt tagtaagtgg ctcgcataaa
      961 agccacttac taaaaatctc aaatttgccc cttattatta aacttttcaa aatgaataaa
     1021 attttaaata tattattatc tttcctttta attactttcc taacaaaagc ccaatcctcg
     1081 tttcttgccg aacagggagt tgctgttttt tgtccggcag atatggactc ggttcgtaca
     1141 ttaccttcat tggcattagt aaaaaacctg caacctcaag gttctgtgcc agacaattgg
     1201 aaaactcgcc ctgttttttc caaaatcaac ggaaaatctg ccgtaaccat aactttcgat
     1261 gaacatgtcg acttatatgg taatggagaa gtgacaggta ctttgaaaag gaacaatacg
     1321 aaggttactc tatggaatac cgataattat acttattcgg ccaaagataa tggaaccagg
     1381 ctttaccagt cacacccctg ggtacttgga gttcgaaaag atgggtcttc gttcggtatc
     1441 attgccgata attcatggaa acaatatttt gacctgtcaa atccgattac cattacttct
     1501 gaaggacctg ggtttagggt tattgttata gaaaaaggaa acccaatgga ggtaataaaa
     1561 accttatcgg aactgaccgg gaaaatgaac ctgcctcctc tttgggcatt gggatatcaa
     1621 caatgtagat tctcctatta tcctgcctca agagtaaaag aagttgcgga tgaatttcgt
     1681 aaccggaaaa ttccctgtga tgttatctgg atggatatag actacatgga taattataag
     1741 atatttactt tcgaccctgc aaaatttcct gatcccaaag gtctgaacga ttatttacac
     1801 agcaaaaaaa tcaaatcggt ttatatgatt gatccaggtg taaaaaaaga gacaggttat
     1861 tctgtttacg aagatggaaa attcggaaat cattgggtaa tcgataaaaa tggaaaagag
     1921 tttaatggca gtgtttggcc gggagcttgt gctttccctg attatacaaa tccggtgaca
     1981 caaagttggt gggcaggatt atataaagat tttatggata agggtgttga tggagtttgg
     2041 aacgatatga acgagccgtc agtattcgat ggtccggacg gaactatgcc tgaagataat
     2101 attcataggg gaggcagtgg acttccacag gatattcatt gccgatacca caatgtgtat
     2161 ggtatgttga tggtaaaatc aagccgcgaa ggaataatgg ctgcgaatcc ggataaacgt
     2221 ccgtttattt tgtcgcgttc aaactttctg ggtggacaaa gatatgccgc aacctggact
     2281 ggcgacaatc gttcgaccga agaacacatg aaattatcta tcccaatgtc cttgaacctt
     2341 agtttatctg gacaaccttt cagtggtccc gatgttggtg gatttgatgg agatggaaat
     2401 gccgatttat tggggaaatg gatggcgtta ggagcctatt tcccatttta tcgaaatcat
     2461 agttcttcaa acacagttaa tcaggagcct tgggcatttg gaacgaaaat agaatctgtt
     2521 agtcgcaccg caattaatcg gcgttacttg cttttgcctt atttatatac tcttttcaga
     2581 gaagcatcag tagatggaac ccctattatg caaccggcat tttttgccga ctttcaggat
     2641 actactttac gaagtgaaga acaaatattc ttgttaggaa aagatttatt agttgttcct
     2701 cgttgggcat ctaatataaa cttcccgaaa ggtgattggg ataaaattaa atttgagaat
     2761 ttcgatgatg gctatcaagc taccgtttta ttacgcgaag gggctattgt gccaatggga
     2821 acgctgatac aaagcacaga ggattataac acagattcag ttacattact tattaatccg
     2881 gcatcagacg gcacagcagc aggtattatg tatgacgatg catcaaatgg ttacggatac
     2941 aaaagtggag attatgatat ccagcagttt tcagcatcga aatacaagga agattcttta
     3001 aaaataacaa tcaacaaaat cgaaggaagc agaaagtcaa ctcgactgtt tcgtattggt
     3061 tatgtaaccg gtaatgatgt tgtatattct gattggtcgt caaatacaac tcaatacatt
     3121 aaatatattc ctgataattt aatgacagtg gatcattcat tatttccatc aatgtttatt
     3181 ggaggaagtt tcaataaatg gaatcccgct tcgatgccta tgcaatatca gggaaatggt
     3241 aaatggaaat ctacgagagt taatctttta gcaggacagc acgaatttaa atttatttct
     3301 ttgaaaaatc gtacaggagt ggaatgggga aatgccgccg gaacaatggg gactgctggt
     3361 attatctcaa acgacactat aggaatcaat tttacattat ccgaaaacgg ggaatatata
     3421 attagtttca atgagtcaac attaaattac tccatactaa aagctccaaa atatgattat
     3481 ttatcgattg ttggcgatgc aactccagct gattggactc cgtccggtat tgcattaatt
     3541 caggattcaa ctaatttgaa tatatttagt tataaggggc aattaaaaga aggtaatttt
     3601 aagttccatg ccgtaaatgg agattggtgc gatggggatt ggttgttagc aacatcgcaa
     3661 gatcagacac ttacatctac ggagtataca gtattcacag gttgcccatc tgatattcag
     3721 gatttaaaat ggaaagtatc aacagctggg agttataata ttacgataaa tcttgaggct
     3781 cgtaccatat caattaaatc gttagactat tttccaaacc tttttttggc agggagtgct
     3841 acagccggcg gttgggattt aagatacact tctgatatga atgccaaccc attaaatcct
     3901 gcaaatttca gttgggcagg aaagttaagt cctggcgaat ttaaaatagg aacaacaaaa
     3961 acatggggca atggttggcc atggattcac ccgaaaaca
//
LOCUS       DAOPYB010000240         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_29718_length_3989_cov_10.850534, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000240 DAOPYB010000000
VERSION     DAOPYB010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29718_length_3989_cov_10.850534"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..2373)
                     /locus_tag="PKH58_12330"
     CDS             complement(22..2373)
                     /locus_tag="PKH58_12330"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNX89859.1"
                     /translation="MTQPQNLNYHQTSDFKKITDLVIRNYKLFAVSLIVTLGLAFFMN
                     RFSVPIYEVSSSILIKEDNMQGRGNMNDYLNSSLFGTNQNFQNELWVLQSSPVIDKTV
                     KNLDLSVTYLRKEGFQYRDAYKNVPFKVLYSPNHVQPINAVFNITFLKNDEFRLVAEA
                     EVASFYLYSTNEYKYQKSDWTFNYVGKTGKLVETPDLSFIIEKDSLSFIPDGDASKYS
                     FQFVDLNALANGYKNAFQYSVVDKKATVIKITLQSESVEKGIDLVNGLMDVYSQQNLE
                     RKNHIASITIDYIEKQLGEISDSLNLTEDNLQRFRSSNQLLNVNEQASGISAQYLELQ
                     NERAELITHKRYYDYVSDYLGKNEDFSNMVVPASMGIQDQILNNLMSELITAQAQRSN
                     LIENKQEKNPLVKKLDIQIANAKRTISDNISAVRQTTDISIDEMNKRIGRIEARISNL
                     PKTQRQLGGIERKYRLNDAIYNYLLEKRAEAKITKASNLPDNIIIETAKASSSPVSPN
                     KKKNYFLALVMGMGLPFGFLILKNLLNNKVETQDDIEKITDAPVVGKILHNYKKSNNI
                     MFEYPKSPIAESYRALRTNLDFYLKGGHRKVILVTSCIESEGKSFTALNIAMSYAQMN
                     RKTILLDFDLRKKKSFFNNETDSHDGISSYLINKVTLEDIILKSPHEKLDYICSGPIP
                     PNPAEMVAFDESYQLIENLKEYYDYIILDTPPLAQVTDGYLLMKMADLKVLVVKYNFS
                     KKNVLSIVMKDLRQKEIGNVCLVLNDNRVKGDQYGYGYGYYKK"
     gene            complement(2399..3208)
                     /locus_tag="PKH58_12335"
     CDS             complement(2399..3208)
                     /locus_tag="PKH58_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX89860.1"
                     /translation="MDSTNQFRYSEYISRYVFLIPLIFCTLPFSSCITQKNVEYLQDE
                     DKGIKSFTEARYEEYRLKPNDELYIQISSIDEAAASIFNNSTSPQSFSVGAIQPYGAS
                     LASYSVDKDGFVQLPVIGNIFVKDKTIPQVIMMVKDSLTNILSQPIVNIKLVNRYVSV
                     LGEVRNPGHFTYSQEKISLYDALGYAGDITDYGNRNEVLLMRNENGKTIRINLDLTSS
                     DILTSKYYFLKPNDLIYVKPLRKKTWGMREFPFAITLSAISTAILLYTVVK"
BASE COUNT         1098 a          802 c          798 g         1291 t
ORIGIN      
        1 ataatcctga cggtacagct attatttctt ataatacccg tacccgtatc cgtactgatc
       61 acctttaaca cgattatcgt tcagcacaag gcatacattg ccgatttcct tttgccttaa
      121 atctttcata acgatcgaca atacgttctt cttagaaaaa ttatatttaa ctaccaacac
      181 tttaaggtcg gccattttca tcagaaggta accatcggtg acctgggcga gaggaggtgt
      241 atccaggatg atataatcgt aatattcttt caggttctca ataagctgat aggattcatc
      301 aaaagctacc atttcggcag ggttgggggg aattgggccg gaacagatat aatcaagctt
      361 ttcgtgagga gacttcagga taatatcttc caatgttact ttgttgatca gataagaact
      421 gatcccgtca tgggagtcgg tttcattgtt gaagaacgac tttttcttcc gcaggtcaaa
      481 atccagtaat atagtcttgc gattcatctg tgcgtagctc atcgcaatgt ttaatgccgt
      541 aaaacttttc ccttcgcttt cgatgcagga tgtaaccaat atcactttcc tgtgcccgcc
      601 cttcagatag aaatcgagat ttgtcctgag cgccctgtat gattctgcaa tgggtgactt
      661 tggatattcg aacatgatat tgttcgattt tttataattg tgcaatattt tccctacaac
      721 cggggcatcg gtaatttttt ctatgtcatc ctgagtctca actttgttgt tcagaagatt
      781 cttcaggatc aggaatccga acggcaggcc catgcccata accagggcca ggaaataatt
      841 ctttttctta ttaggcgaca ccggcgatga ggatgctttt gccgtttcga tgattatatt
      901 atccggcagg tttgatgctt tcgtaatttt ggcttccgcc cgtttctcca gcaaataatt
      961 gtagatggca tcattcaggc ggtacttacg ttcaatgcca cccagttgcc gttgcgtctt
     1021 gggaagattg cttatcctgg cttcaattct gccgatccgc ttgttcatct cgtcaattga
     1081 aatgtccgta gtctgcctga cagcagaaat attatcagaa attgtccttt tcgcgttcgc
     1141 aatctgaata tcaagttttt tgaccagcgg atttttttcc tgtttgttct caatcaggtt
     1201 cgaccgctgt gcctgggcgg tgatcagttc cgacatcagg ttattcagta tttggtcctg
     1261 aattcccata gatgccggga ctaccatgtt agaaaagtct tcgttttttc cgagataatc
     1321 ggacacataa tcataatatc gtttatgggt gatcagctcg gccctttcat tctgcaattc
     1381 aagatactgg gccgaaatgc ctgacgcctg ttcatttaca ttcaacaatt gattggaaga
     1441 acggaaacgc tgcaggttgt cttccgttag gtttagcgaa tcggaaatct cgcccagttg
     1501 tttttcaata taatcgatag tgatgcttgc aatatggttt tttcgttcca ggttttgttg
     1561 ggaataaacg tccatgagcc cgttaacaag gtctatgcct ttttcaaccg attcggattg
     1621 cagtgttatc ttaatcacgg tagctttctt atcgaccacc gagtattgga atgcgttttt
     1681 atatccatta gccaaggcat tcagatcgac aaactgaaaa ctgtatttag aagcatcacc
     1741 atccggaata aaactcagac tgtctttttc aataataaag gacaaatccg gcgtttcgac
     1801 aagtttgccg gtcttgccga catagttgaa ggtccagtca cttttctgat atttatactc
     1861 atttgtacta tataaataaa atgacgccac ttcggcttct gcaaccagcc tgaattcatc
     1921 attttttaaa aaagtaatat taaaaacagc gttgattggt tgcacgtgat tgggtgaata
     1981 cagcactttg aacggaacat ttttataggc atccctgtac tggaaacctt ctttccttag
     2041 ataggtgacc gatagatcaa gattcttgac ggttttatcg atgacaggcg aagattgtaa
     2101 tacccataat tcattttgaa aattctgatt cgttccgaat aaactgctgt taagatagtc
     2161 attcatattg cctctgccct gcatgttatc ttccttgata agtatggaag aagatacttc
     2221 ataaatcggg accgaaaacc ggttcatgaa gaaagccaat cccagagtca ctataaggct
     2281 aaccgcaaat agtttatagt ttcgtattac gagatctgtt attttcttaa aatcacttgt
     2341 ctggtgatag tttaaattct gtggctgcgt cattgtatgt tgatcggggg ttaactttct
     2401 atttcacgac ggtataaagc aaaattgcag tagatattgc agaaagggta atggcaaaag
     2461 gaaattcacg cataccccat gttttcttac gcaagggctt cacgtaaatc aaatcgttgg
     2521 gcttcagaaa atagtatttc gaagtaagaa tatcggaaga agtaagatca aggttgatcc
     2581 tgatagtctt accattttca tttctcatga gcaacacttc gttcctgttt ccgtaatcgg
     2641 taatatcacc agcatatccc agtgcgtcat aaagggagat tttttcctgc gaatatgtaa
     2701 agtgacccgg gttgcgcacc tccccgagaa ccgacacata acggttgacc aatttaatgt
     2761 ttactattgg ctgactgagg atgtttgtca acgaatcctt caccatcata ataacctgtg
     2821 gtatggtttt gtctttaaca aaaatattgc ctatcacagg caactgcaca aatccgtctt
     2881 tgtctacgga atacgaagcc aatgatgccc cgtaaggttg aatggcgcct actgaaaagg
     2941 actggggact ggtactgttg ttaaagatgc ttgccgcagc ttcgtcaatg ctgctgatct
     3001 gaatgtacag ttcatcattt ggcttcagcc tgtattcttc atacctggct tcggtaaatg
     3061 atttaattcc tttgtcttca tcctggaggt attccacatt tttctgggtt atgcaggaag
     3121 agaacggtaa tgtacagaaa atcagtggta ttaaaaatac atatctgctg atgtattcag
     3181 aatacctaaa ttggttcgta ctgtccatga tgaagcaatt tttgtattga ggaataaata
     3241 tactcttttc tcgggctatc tgacggcagg ttcttactta ttacctgaga ataagctggt
     3301 ttccaaattg atcatattat cagcgctctg atttgctgtt gatgtttggg taccggaaat
     3361 agcaccagat ttatttccgg gatttgagac tgatacggaa cctgttccgg tatataaaaa
     3421 gcttcgcctt ctcagtcaca tattgagcga gttgaaccag tatctgattg gctaaaatag
     3481 ccagaatagt aaacgaatac aagtaatttc tattctgata ttctttagat gttgtgtgtc
     3541 attctgaaca atttccacca attcatcttc actgggacat gaatgacttc tcacgggcga
     3601 gatcaaggaa tccgattaat gatgtgctat tccggcgatt ttagatggca aaagaaatga
     3661 caaagatagt accacaatta ttataagatt catgttcaac ggcatatata cccagggatt
     3721 gaatttatta tatctgaatt ttcccatttt gggaaaatga ttcaaattaa accctgtcat
     3781 gacttattga ataatcctgg atttctcctt aatatagaga aatttataag ccatgtcggg
     3841 ggggtacatc attatccctt ttcatcggtt cctcataccc ggcctatcat tctttttttt
     3901 tctaccgatt ttcgaaaaaa aaggccgcaa attcggaggg cactgaaaaa gtatctctta
     3961 ttaaattaat ccttagaaat aagttggca
//
LOCUS       DAOPYB010000241         3982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_29782_length_3982_cov_8.732875, whole genome shotgun sequence.
ACCESSION   DAOPYB010000241 DAOPYB010000000
VERSION     DAOPYB010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29782_length_3982_cov_8.732875"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKH58_12340"
     CDS             <1..500
                     /locus_tag="PKH58_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX89861.1"
                     /translation="LYSIDRTVDVTKVTDFKKGLSVIKFSNDRADGGVVHDSQFPDMD
                     IPFMRVAEAYLTYAEANLRSSNGKAADALKAVNDLRTRSHATPIVGLNLDKVLDEWAR
                     EFYFEGRRRIDLIRFGYFSGTNYNWDWKGGVAAGTSFDSHYDLMPLPPGDLNANSNLK
                     QNKGY"
     gene            531..1757
                     /locus_tag="PKH58_12345"
     CDS             531..1757
                     /locus_tag="PKH58_12345"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HNX89862.1"
                     /translation="MKKINILIVVILSVFGFTACEDAIDPIINDYDSYKTFVLNQPTY
                     SNMTYELTFANAEKDMDALTCVQPNYGFTAAVTYTTQVSFSNTFAAGTFQELPTTVNG
                     EKVNINTLEMDKAIIGLYGGALPNPVVEKEVYVRLKAFISDATPSPLNDTLTVKPVYS
                     NAIKLKIKPYVLPLFPYTDVTPRLWYIVGLDGAWKNEVAGLGSSLIPFGVVDGKKYNL
                     NGDGEFTYTGYFKASAGFKLIRDVGAWAEQWGNAGGEGINNPHRKLTGDSDPSNFKVP
                     ADGYYTITINSIENSATIVPASITPASYTTIGLIGAFTGNDWSSDVVSFTANESANKH
                     IWFTTYTFDGEHQCKLRADSKWDVNWGTGGTIDGNKLYSAMGIGVQGGKNMVQSAGTY
                     IIILNDITGHYTFIRK"
     gene            1830..3257
                     /locus_tag="PKH58_12350"
     CDS             1830..3257
                     /locus_tag="PKH58_12350"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX89863.1"
                     /translation="MKKTIKYIFALAIGCALFASCEDPYAGQEVAEPGAFKQTALQDP
                     NFVATVKANPLTITSDKLTSSVDFITISSKPTLADTAAHFEYKVILSNLENFSVYKTI
                     DTQLSGTSTITATYKQLNDTLKALNPILGEHNAYARVLAYIVKSGTKALYTTGNLPFK
                     VTTYNYPPVAINDLATLPMNSSITIDVLANDTDPEANTLTIASVGTPGHGTAVITSGK
                     IVYTPATGYSGPDYFSYTINDGNGNTISANVDITVLSIVPYTDITPRPYYIIGMANGA
                     WNNSKAGLGVSIYPMSVVAGNKYNTSGDGEFTYTGYFWASRGFKLIRDIGNWDEQWGA
                     NGNDITKPLHNNGGSSDFKVPADGYYTITLNSITNTFSMVPATVTPATYTKMGMIGGF
                     TGWGSDVDMTACESSNNHNWYTTYTFDSDTEGKFRANADWGTNWGANTFPVGLGVGNG
                     PNVPVKAGTYTVIFNDISGCYYFIK"
     gene            3360..>3982
                     /locus_tag="PKH58_12355"
     CDS             3360..>3982
                     /locus_tag="PKH58_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX89864.1"
                     /translation="MKKTIAILTLTFIAISYISGQKYTNPPEWAKNVIWYQIFVERFN
                     NGDPSNDPTAETTTVANMNIVPPKGWKTSSWTGNWFERQPWEQDAKQGFNSVIQSRRY
                     GGDLQGVMNKLDYLQELGITAIYFNPLNDAPSLHKYDARNYHHIDVNFGPDPEGDIKL
                     MATEDPGNPVTWKWTSADKLFLKLVDELHKRGIRVIVDYSWNHTGTN"
BASE COUNT         1228 a          824 c          818 g         1112 t
ORIGIN      
        1 tgttatattc aatcgaccgt actgttgacg ttacgaaagt tactgatttc aagaaaggat
       61 tatccgttat aaaattctca aacgaccgcg ccgatggtgg agttgttcat gacagtcagt
      121 tccccgatat ggacattcct ttcatgcgtg tagctgaagc ttatcttact tacgccgaag
      181 ctaatctccg ttcaagcaac gggaaagctg ctgatgcgct gaaagcagtc aacgacttac
      241 gcacacgctc gcatgcaact ccaattgttg gacttaacct ggataaagta ctggatgaat
      301 gggcccgcga attctatttc gaaggtcgcc gccgtattga tttgatccgt ttcggatatt
      361 tcagcggtac aaattataac tgggactgga aaggcggagt tgctgccgga acatcgtttg
      421 attcacatta cgatctgatg ccactgcctc cgggtgattt gaatgctaat tccaacctga
      481 aacaaaacaa gggttattaa tttgattaat atataataaa cagtatgaat atgaaaaaaa
      541 taaatatatt aattgttgta atcctcagcg tttttggatt tacagcatgt gaggacgcaa
      601 ttgatccgat aatcaacgat tacgacagct acaagacatt tgtcctgaat cagccgacgt
      661 atagcaacat gacttatgaa cttacatttg ccaatgcgga gaaagacatg gacgcattaa
      721 catgcgttca gccgaactat ggttttactg ctgcagttac ttatactaca caggtaagtt
      781 ttagcaacac atttgctgcc ggtacttttc aggaattacc aaccacggta aatggtgaaa
      841 aagtcaatat caacacatta gaaatggata aagccatcat cggtctttat ggtggtgctt
      901 taccaaatcc ggttgttgaa aaggaggtat atgtcagatt aaaagcattt atttctgatg
      961 caaccccttc acctctgaac gatacactga ccgttaaacc ggtttattca aacgcgatca
     1021 aactgaaaat caaaccttac gtattgccat tattccctta taccgatgta acaccaaggt
     1081 tatggtatat cgtaggactt gatggtgcat ggaaaaatga ggttgcaggt ttaggcagtt
     1141 cattgattcc gtttggagtg gttgatggca agaaatacaa tctgaacggc gatggtgaat
     1201 ttacctatac cggttatttt aaagcgtccg ctggcttcaa actcatcaga gatgttggag
     1261 catgggcaga gcaatggggt aacgcaggtg gtgaaggtat caacaatcct catcgtaaac
     1321 tgacaggtga tagtgacccg agcaacttta aggttcctgc tgatggatat tataccatta
     1381 ccatcaactc aattgagaat tccgcaacca tcgttcctgc ttcaatcaca ccggcaagct
     1441 atacgactat cggtctgatt ggtgccttca ccggaaacga ctggagttcg gatgtagtaa
     1501 gtttcaccgc aaatgaaagt gcaaataaac acatctggtt cactacctac actttcgatg
     1561 gcgaacatca atgtaagctg cgtgcagact ccaagtggga tgtaaactgg ggtactggag
     1621 gtacaattga tggcaataaa ttatattctg caatgggtat aggcgtacag ggtggcaaga
     1681 atatggtaca atctgccgga acatacatta tcatcctgaa tgatattacc ggtcactata
     1741 ctttcatcag aaaataaaaa ttatatagtt ccgcctttcg ggcggaacta tataataatc
     1801 attacttcaa cattaaaaaa ataatgaaaa tgaaaaaaac gataaaatat atttttgcat
     1861 tagccattgg atgtgcactt tttgcttcct gcgaagatcc ttatgcaggt caggaagttg
     1921 cagaaccggg agctttcaag caaacagctt tgcaggaccc taactttgtt gctaccgtaa
     1981 aagctaatcc tctcacaatt accagtgata agttaacttc ttctgttgat tttatcacaa
     2041 tatcttctaa accaacttta gctgatactg ctgcacattt tgaatataaa gtgatccttt
     2101 caaatcttga aaacttcagc gtgtacaaaa cgattgatac tcagcttagt ggaacatcaa
     2161 caattacagc tacttacaaa cagttgaatg acaccctcaa agccttaaat ccgattcttg
     2221 gcgaacacaa tgcttatgca cgcgttctgg cttacattgt aaaaagcgga acaaaggctc
     2281 tctacactac cggtaatctg cctttcaagg ttacaaccta caattatccg ccggttgcaa
     2341 tcaacgatct ggctacctta ccgatgaaca gttcaattac tattgacgtt ctggctaacg
     2401 acacagatcc ggaagccaac acgttgacaa ttgcaagtgt aggtacacca ggtcatggaa
     2461 ctgctgtaat aacaagcggt aaaattgttt atacacctgc taccggatat tcgggtcctg
     2521 attatttcag ctacacaatc aacgatggaa atggaaacac catttctgct aatgtggata
     2581 ttaccgttct ttctattgtt ccttacacag atattactcc aagaccatac tacatcatcg
     2641 gcatggctaa cggagcatgg aataattcaa aagcgggtct gggtgtatcc atttacccga
     2701 tgagcgttgt tgccgggaat aaatacaaca catccggcga tggtgaattt acttataccg
     2761 gttatttctg ggcttcaaga ggatttaagc tgatacgcga cattggaaac tgggacgaac
     2821 agtggggagc taatggtaat gacattacca aaccattaca caacaatggc ggttcgagcg
     2881 acttcaaagt tcctgccgat ggttattaca ccatcacatt aaattctatc accaacacat
     2941 tctcaatggt tcctgcaact gttacacctg ctacttacac aaaaatgggt atgattggcg
     3001 gatttaccgg ttggggaagt gatgtggata tgacagcttg tgaatcatca aacaaccata
     3061 actggtacac tacttataca tttgattcag atactgaagg aaaattccgt gctaatgccg
     3121 attggggtac taactggggc gctaacacct tccctgtagg tttgggagta ggaaatggtc
     3181 ctaatgttcc tgttaaagca ggtacttaca ctgtgatatt taatgatatc agcggatgct
     3241 actattttat taagtaaaaa atacagctaa aaaaacaaag gctgacttaa caaagtcagt
     3301 ctttgttttt tatacaaaat tcattaaact tgagaaagta ttttaaatat tgatttttta
     3361 tgaaaaagac aatcgcaatt ctgacactta cctttattgc aatcagttat atctctgggc
     3421 agaaatatac gaatcctccc gaatgggcaa aaaatgtaat ctggtaccag atttttgtcg
     3481 aacgtttcaa caatggtgat ccatccaacg atccgacagc agagacaaca acagtggcaa
     3541 acatgaatat agttcctcca aaagggtgga aaacatcctc ctggacgggc aactggtttg
     3601 aaagacagcc ctgggaacag gatgcgaaac aaggtttcaa ctctgtcatc caatcccgcc
     3661 gctacggagg tgatttgcag ggcgtgatga ataaactcga ctatcttcag gagttgggta
     3721 ttaccgccat ttatttcaat ccgctgaatg atgcgccctc cctgcataaa tatgatgcac
     3781 ggaattatca tcatattgat gtcaactttg gtcccgatcc tgaaggcgat ataaagctga
     3841 tggcaaccga agatcccggc aaccctgtaa cttggaaatg gacttcggcc gataaactgt
     3901 ttctgaaact ggtggacgaa cttcacaaac gcggtatccg tgttattgtg gattattcgt
     3961 ggaatcatac cggaaccaac tt
//
LOCUS       DAOPYB010000242         3975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_29863_length_3975_cov_3.324490, whole genome shotgun sequence.
ACCESSION   DAOPYB010000242 DAOPYB010000000
VERSION     DAOPYB010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29863_length_3975_cov_3.324490"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PKH58_12360"
     CDS             <1..830
                     /locus_tag="PKH58_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX89865.1"
                     /translation="IEMIKTLKLKNYSENTIRTYTMMFSRFMQYYGFRNPETLSDSDI
                     REYLLHLIEKKQVSQSFQNQAINAIKFYYEQVLGRASNTYYVQRPKKEMRLPSVLSER
                     EVASLLSQVRNLKHRAALSLIYSAGLRISELINLSISDIDAKRGQVIIRQGKGRKDRI
                     SVLSANILDLLRKYYRRYHPETWLFEGQYGGQYSANSIQTVFRQAKEKAGIMKHATVH
                     TLRHSFATHLLERGTDLRYIQELLGHASTRTTEIYTHITPKGFRKIISPFDNLDLGK"
     gene            1291..1926
                     /locus_tag="PKH58_12365"
     CDS             1291..1926
                     /locus_tag="PKH58_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89866.1"
                     /translation="MWQIFIGSLILSVIHALIPNHWIPLIAISKTEKWTNKETLFATF
                     ITGVSHTLSTIIIGILIGFIGYKLSESYSYITKVAAPIILVVIGIIYMIIDLKTSHHH
                     HHHFDVDESKKNNKKSMWAILVSLSIAMFLTPCIEIEAYYFQAGTIGWLGIFIVSAVY
                     LCVTLLVMFILVYLGLKGVSRFNSHYLEHHEKRITGMVLILLGLLAYFVEF"
     gene            1937..2800
                     /locus_tag="PKH58_12370"
     CDS             1937..2800
                     /locus_tag="PKH58_12370"
                     /inference="COORDINATES: protein motif:HMM:NF021357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HNX89867.1"
                     /translation="MKWITRERPKIDRIACPWLIKRFIDKDAEIIYVPFGDVISKAKE
                     LNAIPFDIPDVEFTHYGDHCTFDYFIEKYKITDPALLTIAVIVRGADTDRHDIANQSS
                     GLWAISAGLAHNVTDDQQLLDKGMLVYDALYSWAKYLQQEKHTQSPFENLLLGIFNKF
                     LKEKSGAKKKTPAWAKELKEIIQDQIDINLALSLKDISKNLDVHPSYLSREFSKYFDD
                     LSFGEYIRKLRIEKAIQLIHSTNYSLTEIAYLTGFSDQSHFTRIFKKHTGKNPSTFKK
                     SIKKGQPRTKS"
     gene            2861..3319
                     /locus_tag="PKH58_12375"
     CDS             2861..3319
                     /locus_tag="PKH58_12375"
                     /inference="COORDINATES: protein motif:HMM:NF021357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HNX89868.1"
                     /translation="MKWITRERPKIDRIACPWLIKNFVDKEAEFIFVPTDQVLSKAKE
                     LDAIPFDIPKVEYSHEGEFCTFDTIIKKHNIKDPALLQIAIIVRGADSDRFDLAQQSA
                     GLWAISAGLSYNYRNDFEMLEVGMKIYDALYSWAKYASDEHHTWNHDLVV"
     gene            3316..>3975
                     /gene="chrA"
                     /locus_tag="PKH58_12380"
     CDS             3316..>3975
                     /gene="chrA"
                     /locus_tag="PKH58_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNX89869.1"
                     /translation="MTPEVKPKYSLLQLVLYFLKLGYSGFGGPVALVGYMYRDLVENR
                     KWITDEEYKEGLALAQLAPGPLAAQLGIYIGFVHYGIIGATIAGIAFILPSFIMVVVL
                     GIAYKLFGGLPWMQSIFYGIGAAVIGIIAISSYKLTTKSIGKFNLESFSNNWMLWLLF
                     LVAAIITAFTQTEKVFLFIASGILYMLIKAPPRWVKKPVALNLMLLTGIGFWQFDWGT
                     LW"
BASE COUNT         1189 a          817 c          832 g         1137 t
ORIGIN      
        1 ccattgagat gataaagact ctgaagctga agaactacag cgaaaacacg atccggactt
       61 acacgatgat gttcagccgg ttcatgcagt attacggttt caggaatccg gagactcttt
      121 ccgacagtga tatcagggag tacctgcttc acctgatcga aaagaagcag gtttcgcagt
      181 cgttccagaa ccaggcgatc aatgccatca agttctatta tgagcaggtt ctcggcaggg
      241 cttccaatac atattacgtc cagcggccga agaaggaaat gcgtctccca tcggttctga
      301 gtgagagaga ggtggcatcg cttctgtcgc aggtcagaaa cctgaagcat cgtgctgccc
      361 tgtcgctgat ctattcagca gggctgcgta tcagcgaatt gatcaacctg agtatttccg
      421 atattgatgc aaagcgcggg caggtgatca tccggcaggg caaagggcga aaggatcgta
      481 tatcggtttt atccgctaac atcctggatc tcctcagaaa atactaccgg cgttatcatc
      541 ccgaaacatg gttattcgag ggacagtacg gtgggcaata ttcggcaaac agcattcaga
      601 cagttttccg gcaggcgaaa gagaaggcgg gtatcatgaa gcatgccacg gtacatactt
      661 tgcgtcatag ctttgcgacc cacctgctgg agcgcgggac cgatctccgg tacattcagg
      721 agctgctggg ccatgccagc acccgtacta cggagattta cacgcatatt acacccaaag
      781 gtttccggaa aatcatcagt cctttcgata atctggattt gggaaaatag gcaaatgttt
      841 tgtcattaat ttttcatttt gtatattcgt ggtcatatat agacgcacac gtcatgtcga
      901 atatattatg cagataccta ttccgggcca ggagtaatcc gttgcggata tcaccaaccg
      961 acaacttgtg ggtactcccg ctctgtttgt tgtacaccca caactttcgc ctgagaacat
     1021 gtaaaatata tcagcaaccg ttgtgggtac acagtcgtta gcagcaaggt aacttgacga
     1081 cacggataga aaaattccta gacaacgaaa cgaagaagaa agataacccc ctacgcacaa
     1141 aagcagagta tgtcagccct ttccctgcat gcctcccttc gctgccatac acagcttttc
     1201 ttgccccccg ctcgctttgt agtaatgttt tcttactttt acattcatta gtttggatta
     1261 agtgagtaat aacattaata attacagaaa atgtggcaga tatttatagg tagtcttatt
     1321 ttgagtgtga ttcacgctct cattccgaat cattggattc cattgatagc catttcaaag
     1381 acagaaaagt ggacaaataa agaaactctg ttcgcaacat ttatcacagg ggtatcacat
     1441 actttaagta ctatcatcat aggaatacta ataggattta tcggctacaa gctatctgag
     1501 agttatagct acatcaccaa agtagcagca ccgataatct tggttgtgat tggtataatc
     1561 tacatgataa ttgacctaaa aacatctcat caccatcatc atcattttga tgttgatgaa
     1621 agcaaaaaaa acaataagaa atcaatgtgg gcaattcttg tatctctaag cattgctatg
     1681 ttcttaaccc catgtattga aatagaagcc tattattttc aagctgggac gataggttgg
     1741 ttagggattt tcatagtttc tgccgtttac ctttgtgtta cactattggt aatgtttatc
     1801 ttggtatact taggtttaaa gggcgtgagt aggtttaact ctcactacct agagcaccat
     1861 gagaaaagaa taacagggat ggtattgatt ttactaggat tattagccta tttcgttgaa
     1921 ttttaatagg tgaaaaatga agtggattac cagagaacga ccaaagattg accgaattgc
     1981 ctgtccgtgg ctcattaagc gtttcatcga caaggatgca gaaataatat acgtcccatt
     2041 tggtgatgta atctctaagg cgaaagagct gaatgcaatt ccctttgaca tccccgatgt
     2101 tgagtttacc cattatggtg accattgcac ttttgactac ttcattgaga agtataaaat
     2161 taccgaccca gctcttctta caattgcagt aattgtgcgt ggtgctgata cagataggca
     2221 tgatatcgca aaccaatcaa gtggtctatg ggcaatctca gctggacttg ctcataatgt
     2281 tactgacgac caacagctac tagataaagg aatgttggtt tacgatgccc tgtacagctg
     2341 ggcaaaatac ctacagcaag aaaagcatac ccaaagccca tttgagaacc tactccttgg
     2401 tatttttaat aaatttctaa aagagaaatc aggtgcaaaa aagaaaacac cagcatgggc
     2461 taaggagctg aaggaaatca tacaagacca gattgacatc aacctagcac ttagcctaaa
     2521 agatatttct aagaatttgg atgttcaccc ttcataccta tcaagagagt tctctaagta
     2581 ttttgatgac ctttcctttg gggagtacat ccgaaagcta cgaattgaaa aagccattca
     2641 gctaatccat tccactaact actcgctcac tgagatagca tacctcacag gtttctctga
     2701 ccaaagccat tttaccagaa tcttcaaaaa gcatacaggt aaaaatccat ctactttcaa
     2761 aaaaagtatt aagaaaggtc aaccacgtac aaaaagttaa ttccattcta ttttctcgca
     2821 aaccattttt gcacttttgc ttatataaat taaccgtgct atgaaatgga taacgagaga
     2881 acgaccgaag attgaccgaa tagcctgtcc gtggttaatt aaaaactttg tggataagga
     2941 ggcggagttt atttttgtac caacagacca agtgctatcc aaggctaaag agctggatgc
     3001 aattcccttt gatattccca aggtggagta ttcacacgaa ggcgaatttt gcacctttga
     3061 taccatcatt aaaaagcaca acatcaaaga cccagcattg ctacaaattg caatcatagt
     3121 aagaggtgct gactctgacc gatttgattt agcgcaacaa tctgctgggc tttgggctat
     3181 atcggctgga ctttcctaca actataggaa tgattttgag atgctggaag taggaatgaa
     3241 aatttatgat gcgctataca gctgggcaaa gtatgcatcc gatgaacatc acacatggaa
     3301 tcatgactta gtagtatgac accagaagtt aaaccgaaat atagcctact tcaactcgtt
     3361 ttatattttc taaaactagg ttactctggt tttggaggtc ctgttgctct agtgggctat
     3421 atgtatcgtg atttagtgga aaatcgtaaa tggataaccg atgaagagta taaggagggt
     3481 cttgcacttg cacagctcgc acccggtccc ttagctgctc aacttggaat ctatattggc
     3541 tttgttcact acggtataat cggggctacc attgctggta ttgcatttat tttaccatcg
     3601 ttcataatgg tagttgttct gggtattgct tacaagctat ttggaggatt accttggatg
     3661 caatcgatat tttacggcat tggtgctgct gttatcggga ttatagcgat tagctcctac
     3721 aagctaacca cgaaatccat cggcaagttc aatcttgaat cgttctcaaa taactggatg
     3781 ctttggctac tatttttagt ggcagcaatt attacagcgt ttacgcaaac tgaaaaggta
     3841 tttcttttta ttgcatcagg aattctttac atgctcatca aagcacctcc aaggtgggta
     3901 aagaaacctg tagctttaaa tctaatgctc ttgactggaa tcggattttg gcaatttgat
     3961 tggggtactc tttgg
//
LOCUS       DAOPYB010000243         3925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_30451_length_3925_cov_9.073385, whole genome shotgun sequence.
ACCESSION   DAOPYB010000243 DAOPYB010000000
VERSION     DAOPYB010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30451_length_3925_cov_9.073385"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PKH58_12385"
     CDS             complement(<1..1010)
                     /locus_tag="PKH58_12385"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNX89870.1"
                     /translation="MATKVYFPTVEKIKFEGKESKNPLAFRYYDAEKVVYGKTMKEWF
                     KFSMAWWHTLCADGGDPFGGQTQKHPWVGAADALQAAKEKMDAGFEFMQKAGIEYYCF
                     HDIDLISEGNSIDEYEANLKAIVAYAKQKQAETGIKLMWGTANVFSHARYMNGASTNP
                     DFDAAARAMVQIKNAMDATIELGGKAYVFWGGREGYMSLLNTNMKREKQHMGMMLTKA
                     RDYARAKGFKGVFLIEPKPMEPMKHQYDVDTETVIGFLKEFGLENDFKINIEVNHATL
                     AGHTFEHELQCAVDAGMLGAIDANRGDVQNGWDTDQFPIDIFELTQAMLVILQGGGMQ
                     GGG"
     gene            complement(1080..2555)
                     /locus_tag="PKH58_12390"
     CDS             complement(1080..2555)
                     /locus_tag="PKH58_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNX89871.1"
                     /translation="MYLLGYDLGSSSVKASLVNAATGECVSTAFFPKKEMEIISLKKG
                     WAEQNPDLWWENLKAATKSVLEESAVNPSDIKAIGISYQMHGLVLVDENMKAIRNSII
                     WCDSRAVPFGEKAFEALGAEKCLSHLLNSPGNFTLSKLAWVKENEPQNFSRIKYYMLP
                     GDYIAMRMTGKPCTTASGLSEGMGWDFETEKPASFLFDYFGFEESTVPEIVSTFGHQG
                     SLTVGVAQELGLAEGTPVTYRAGDQPNNALSLNVLNPGEIAATAGTSGVVYGVNSDSK
                     YDPQSRVNSFAHVNHTEKDTRIGVLLCINGTAILNTWIKNNVAPEGISYAEMNELAAA
                     IPVGSEGVSIVPFGNGAERVLGNKEPNCSIHGVNFNRISKAHLIRAAHEGIAFSFKYG
                     MDIMNQMGIETKTIRAGHANLFLSPIFRQTLANITGATIELYNTDGSVGAARGAGIGA
                     GIYKNPEEAFATLKKIMVITPQSDEAEATMEAYKQWINKIN"
     gene            complement(2598..3308)
                     /locus_tag="PKH58_12395"
     CDS             complement(2598..3308)
                     /locus_tag="PKH58_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNX89872.1"
                     /translation="MTTENIYNPHVSVDCVVFGFDGENLKVLLIERNIQERNENYNDK
                     KLPGSIIYNDEDLDEAATRVLTELTGLKNIYLSQFRSFGNPNRINDPRDILWLENTMK
                     LKIGRIVTVGYLALIKIDRKIQVLSDNTVANWYDLKDLDNVRIAFDHAEIIKTALEQV
                     RNKLDRDPQLFFELLPRKFTMSQLRVLYDKIHNVRSDIRNFQKKVAQWPYVVPLDETE
                     TGVPHRAARLYKFDKKKK"
     gene            complement(3636..>3925)
                     /locus_tag="PKH58_12400"
     CDS             complement(3636..>3925)
                     /locus_tag="PKH58_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX89873.1"
                     /translation="QWLAMEMAMKYGDKVRVNALAPGFFIGDQNRAVLINPDGSLTDR
                     SQKVLANTPMRRFGDINELNGAVQFLCSDAASFITGVVLPVDGGFSMFSGV"
BASE COUNT         1003 a          889 c          812 g         1221 t
ORIGIN      
        1 ccgccaccct gcatgccacc gccctgaaga ataaccaaca tagcttgtgt caattcaaaa
       61 atatcaatcg ggaattgatc tgtatcccaa ccattctgta catcaccgcg gttagcatcg
      121 atagctccta acatgccggc atcaactgca cattgtaatt cgtgttcgaa agtgtgtccg
      181 gccaaagtgg cgtggtttac ttcaatgttg attttgaaat cattttccaa accaaattct
      241 ttcaggaagc caatcacagt ctctgtatcg acatcatatt gatgtttcat tggttccatc
      301 ggttttggtt caatcaggaa tacacctttg aaaccttttg cacgtgcata gtcgcgggct
      361 ttggtaagca tcattcccat gtgttgtttt tctcgtttca tattggtgtt tagcaagctc
      421 atatagcctt cacgacctcc ccagaatacg tatgctttac caccaagttc gatagttgca
      481 tccatggcgt tcttgatctg aaccatggcg cgggcagctg catcaaaatc aggattggtg
      541 ctggcaccgt tcatataacg tgcgtgagaa aaaacattag cagttcccca catcaatttg
      601 attccggttt cagcttgttt ctgttttgca taagcgacaa tagctttcag attagcttcg
      661 tattcatcaa tactattgcc ttcactgatt aagtcaatgt cgtggaagca atagtattca
      721 attcctgctt tttgcatgaa ttcgaatcca gcatccattt tttcttttgc tgcttgcaat
      781 gcatcagctg caccaaccca cgggtgtttt tgtgtttgtc caccaaatgg atcgccacca
      841 tcagcacata atgtgtgcca ccaggccata ctaaatttga accattcttt catggttttt
      901 ccgtaaacca ctttttcagc atcgtaatag cgaaatgcca atgggttttt actttctttt
      961 ccttcaaatt taattttttc cactgttggg aaatatactt ttgttgccat aatacaaatg
     1021 cccccacgcc ccctgtagga gagtttttgg gcggtttttt aactgttaat atacgatgat
     1081 taatttattt tatttatcca ttgtttgtaa gcctccatgg tagcttctgc ttcatcagac
     1141 tgtggagtaa ttaccataat tttcttcagg gtagcaaatg cctcttcggg atttttataa
     1201 atgccggctc cgatgcctgc accgcgtgcc gcgcctaccg aaccatcggt attatagagt
     1261 tcgatggtag ctccggtgat atttgccagt gtttggcgga atatcgggct tagaaaaaga
     1321 ttggcatgtc cggcacgaat ggtttttgtt tctataccca tttggttcat gatatccatg
     1381 ccgtatttga atgagaaggc aataccttcg tgcgcagccc ggatgaggtg agccttgctg
     1441 atacggttga aattaacacc atgaatggag caattaggct ctttattgcc cagtacgcgt
     1501 tccgcgccat tgccaaacgg aacgatactc acgccttcgc tgccaacagg aattgccgcg
     1561 gccagttcat tcatttcggc gtaactgatc ccttcgggcg ccacattgtt tttaatccag
     1621 gtgttcagaa tagctgtacc gttaatgcac agaagtaccc cgatgcgtgt atctttttct
     1681 gtgtgattga catgagcaaa cgaattaacc cgtgattgcg gatcgtattt cgaatcactg
     1741 ttaactccgt aaaccacacc cgaggttcct gccgtggcag caatttcgcc ggggttcagc
     1801 acattcagcg acaaggcatt attgggctga tcgccggcac gataagtcac aggcgttcct
     1861 tctgccagtc cgagttcctg agccacaccg actgtcaggc tgccctgatg gccgaatgtt
     1921 gatacaattt caggaacggt agattcttca aatccaaaat agtcaaacag aaaggaagca
     1981 ggtttttcag tttcgaagtc ccatcccatt ccttcggaca agcccgaggc agtggtacag
     2041 ggtttaccgg tcatacgcat ggcaatataa tcacccggaa gcatatagta ttttatccgg
     2101 ctgaaatttt gcggttcgtt ttcttttacc caggcaagtt tcgacagggt aaaattgccc
     2161 ggagaattga gcaggtgtga caggcatttt tcagctccta gagcttcaaa tgctttctct
     2221 ccgaacggaa cagcgcggct gtcgcaccag ataatagaat ttcggatagc cttcatattt
     2281 tcatctacca gcaccaatcc gtgcatttga tacgagatac cgatagcttt gatatccgac
     2341 ggattgacgg cgctttcttc caaaactgat tttgttgccg ctttcagatt ttcccaccag
     2401 aggtcggggt tttgttcagc ccagcctttt ttcaatgata taatttccat ttcctttttc
     2461 gggaaaaaag ctgtagaaac acattcgcct gttgccgcat ttaccagact tgccttaacc
     2521 gatgagcttc ccaaatcata acctaataaa tacatatatt cctgtttttt aatttgttgt
     2581 aaatcaattg taaactatta tttctttttt ttgtcgaact tgtaaagtct tgcagccctg
     2641 tggggtacac ccgtttcagt ttcgtcgagc ggaaccacat aaggccactg agcaaccttt
     2701 ttctgaaaat tgcgaatatc tgaacgcacg ttgtgaatct tatcatagag gacccggagc
     2761 tgcgacatgg tgaatttgcg tggaagcaat tcaaagaaca gctgcggatc gcgatccagc
     2821 ttatttcgaa cctgctcgag ggctgtttta ataatttcgg catggtcgaa tgcaatccgg
     2881 acattgtcca gatctttcag atcataccag tttgctacgg tattatccga tagtacctga
     2941 attttccggt caattttaat gagcgccaga taacctacgg tgacaattct gccaattttc
     3001 agtttcatgg tgttttcgag ccataaaatg tcacgcggat cgttgatacg gttggggtta
     3061 ccaaaacttc ggaactgact gagatagatg tttttcagac cggtaagttc tgttagcaca
     3121 cgcgtcgcgg cctcgtcaag gtcttcatcg ttgtaaatga tgcttccggg aagtttttta
     3181 tcattatagt tttcgtttct ctcctgaata ttacgttcaa taagtaatac cttcagattt
     3241 tcaccgtcaa aaccaaaaac gacacagtcg actgaaacgt gcggattgta aatattttct
     3301 gttgtcataa tacttggtgt tgttcggtgc aaaaataact attttgttta aatatcgttc
     3361 cggctcaatg tatgttatat aacacatttt tagcattttg aatatagtat tttttacaat
     3421 atcaataaat ttattattgt tatttataca atatgtattt acagagcatt ataaacactt
     3481 cagacaaaat cttagacagg atttattacc agtaagttgc agttgaatca accgtaaaat
     3541 tattttgata ttttaggtag aataccttta aaaaacaaac aaccggtatc agttgaccgg
     3601 ttgtttggaa tttgttagtt gatattgcta ttcacctaca ctccgctgaa catgctgaaa
     3661 cctccatcca cgggcaaaac tacgccggtg atgaatgaag cggcatcgga acagagaaac
     3721 tgtacggctc cgttcagttc gttgatatcc ccgaatcggc gcatgggtgt gttggcaagc
     3781 actttctggc tgcggtcggt gagtgagcca tcgggattga tgagaaccgc acggttctgg
     3841 tcgccaatga aaaaccccgg tgccagcgca ttgacacgta ctttatcgcc gtacttcatg
     3901 gccatttcca tagccagcca ttggg
//
LOCUS       DAOPYB010000244         3920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_30502_length_3920_cov_7.382924, whole genome shotgun sequence.
ACCESSION   DAOPYB010000244 DAOPYB010000000
VERSION     DAOPYB010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30502_length_3920_cov_7.382924"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            714..2273
                     /gene="istA"
                     /locus_tag="PKH58_12405"
     CDS             714..2273
                     /gene="istA"
                     /locus_tag="PKH58_12405"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNX89874.1"
                     /translation="MSNTKITMLKLKRILQLLAAGISQREISNKVGISRSSITAYKER
                     ADSSGKSYQELLQMSDSDVVSVLQRKDYRPGKDARYDKLEPLLEEYSRDITQNRSTYE
                     CLWTEYLKLHPEGYGYTRFKAYIQDYIKSHHYSYHNVYAPGFEMQVDFAGDKLNLVDR
                     RTGLITEVVVLCCILPYSSIAFIMALYNATQECFYYGLSKALSYFGGIPESVKSDNMR
                     QWVIRSDRYEPTFSESTMQWSVHYDTELVATRPVHPKDKAPVEGLVNKTYQFVYSRIR
                     NEEYEALDSLNARIFELTEEFNRRNMTGRTYSRMELFEKEEKPLLKPLPAEPFMLKHR
                     KEFRVPSNYHVQIGKERHSYSVPYQYVNQKATVVFDQESVEVYVDFNRVAIHRRDLKA
                     NGYTTEPSHMPENHRAYQKSKEYNAAYYLGQAHHIGPNTTTVIERILASQPFIQQAYR
                     SSQGILSLSRKYPVERIEAACGRACGTTAAVSYTAIKRILELNLDKEQIDTDTSHIPL
                     HENLRGASFFE"
     gene            2301..3035
                     /gene="istB"
                     /locus_tag="PKH58_12410"
     CDS             2301..3035
                     /gene="istB"
                     /locus_tag="PKH58_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNX89875.1"
                     /translation="MNNQETLIQLESLKMKGMAQAFSAIMNLAPHNRPPLEQIMAKMV
                     ESEILFRNERKMTMYLKTSKLRYNSVLEEVICSEQRNLTKEQLLELSDCSFVKRAENI
                     LITGKTGCGKSYLACAIGKQACHMGIRTEYFAMNKFIEKIAMAKIEGSFIKVINHIEK
                     NDLIIFDDFGLQPLDNNSRLALLQILEDFYERKSVIITSQLPVKNWYDYIGEPTLADA
                     IMDRITSNSVRVEILGESMRRKQKKN"
     gene            complement(3201..3854)
                     /locus_tag="PKH58_12415"
     CDS             complement(3201..3854)
                     /locus_tag="PKH58_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89876.1"
                     /translation="MYKICLIFFFFTTNISYSQINKQLDTTITIDDTTKVHFKSISKK
                     QYFSTKDDRSISFVKLKSIIKQNKSIFIKLQNNAYIELKDSSDEEKTDYVRFSYMGSF
                     NNINFHLFTASYYEIGKCILINDSTGQKYEMFGEPKLSPSSKYFINASGIFNYDPFPN
                     IIQLWNCSNGSLKLLFDFYPSDWVPEGIKWIEGNTIYFIQSFSKNINKYASIQILNN"
BASE COUNT         1341 a          724 c          740 g         1115 t
ORIGIN      
        1 actcaagctc aggaataaga caatatatga catcaataac agagtgtcgt atgttaaatg
       61 ctgtttagac tttatatctg gaatgacgga caactttgct attaaagtgt atgacgaaat
      121 tatatcattt taaataagca acatacagga tattgtggta aataataagc ttgaagaacc
      181 ctcagtactt ttatgcccga caagctaaat attgcatcaa cttctaagct gtcgatcaaa
      241 actataacaa aacaatagtt tcacaaagag actagaaagt ggtttcaaaa caatcattta
      301 tcataaagtt atcatactcg taaccctcgg gttgaaaact tataatgaaa attctggtaa
      361 tagattaaaa gtattaatgt atttggtaaa acctacttta ttgtatcaaa tataagaatt
      421 gtcttagtgt gacgttaacc aatatcaatc ttttcataca aaatatttga aatgaagtta
      481 cattaaatca ttacagattt atttgtaata ttgacacaga ttaaaaataa acatttagta
      541 gattaaaata tattgataat atcacaccaa atgtgccagt catatcactc acaaatgtac
      601 cgggtgtatc actcacaaat gagccaggcg tatcacccac aaatgtacca caataaaggg
      661 atgtaatctt gaaggacgct cctgtgtatt tttgaaaaaa atacactacg gagatgtcaa
      721 acacgaagat tacaatgcta aaacttaaaa ggattttaca attactggca gcaggaatct
      781 ctcaacggga gatcagcaac aaggttggaa taagcaggtc atctataaca gcctacaagg
      841 agagagccga ttcatccggc aagagttatc aggaactttt acaaatgagt gactctgatg
      901 tggtctcagt gcttcagcgc aaggattacc gtccgggtaa agatgcccgt tatgacaagc
      961 ttgagcctct tctggaagaa tatagccggg atataacaca aaatcgtagt acctatgagt
     1021 gtctctggac tgaatatctg aaattacatc ctgaaggcta tggatacaca cgtttcaagg
     1081 catacatcca ggactacatc aaatcccatc attattcata tcacaatgta tatgccccgg
     1141 ggttcgagat gcaggttgat tttgccgggg acaagctgaa tcttgttgat cgtagaacgg
     1201 gattaatcac agaggtagtc gttctatgct gtatcctccc atatagttcc attgccttca
     1261 taatggcact ttacaatgca acccaggaat gtttttatta cggactatca aaagcactgt
     1321 catattttgg cgggataccg gaatcagtca agagtgacaa catgcgtcaa tgggtgatac
     1381 gttcggacag gtacgagccg accttcagcg agtccaccat gcaatggagc gttcactatg
     1441 atacggagct tgtcgccacg aggccggttc atccgaagga taaggctccc gttgagggat
     1501 tggtgaacaa gacatatcag ttcgtttatt cccgtatacg gaatgaggag tatgaggcgc
     1561 ttgattcgct taatgcacgc atatttgagc tcaccgaaga gttcaacagg cgtaatatga
     1621 cggggcgcac ctacagccgg atggaacttt tcgagaagga ggagaagcca ttactcaagc
     1681 ctcttccggc agagccattt atgttaaaac atagaaaaga gttccgtgta ccttccaact
     1741 atcatgtcca gataggaaaa gagagacact cttacagcgt tccataccag tatgtaaatc
     1801 agaaagccac cgtagtattt gaccaggaaa gcgtggaagt gtatgttgac tttaacaggg
     1861 tggccattca cagacgggat ctgaaggcca acggatacac gacagagcca tctcatatgc
     1921 ccgaaaatca ccgcgcatat caaaaaagca aagagtataa tgcagcatac tatctcggac
     1981 aagctcacca catagggcca aatacaacaa cggtaataga acgcatcctt gcctcacaac
     2041 ctttcataca gcaggcttac agatccagtc agggtatatt gtcactgtca aggaagtatc
     2101 ctgttgaaag gatagaggca gcgtgtggga gggcttgtgg tactacggca gctgtaagtt
     2161 atactgcaat aaaaagaata ctggaattaa accttgataa agagcaaatt gataccgata
     2221 catcacatat acccctccat gaaaacctta ggggagcatc tttctttgaa tagcaatcac
     2281 tcacaaaaaa tataattatt atgaacaacc aagagacact gatccaactc gaatccctga
     2341 aaatgaaggg tatggcacag gccttctctg ccattatgaa tcttgcacct cacaacaggc
     2401 ctccccttga acagataatg gcaaagatgg tggagtcgga gatccttttc cgaaacgaac
     2461 gtaagatgac aatgtatctt aaaacaagca aattgagata caacagcgta cttgaggagg
     2521 taatatgttc ggaacagcgt aaccttacca aagaacagtt actggaatta tctgactgca
     2581 gctttgtaaa acgagccgaa aatatactca taacgggtaa aaccggttgc gggaaaagct
     2641 atcttgcctg tgccatcgga aagcaggctt gccacatggg gatacgaacc gagtacttcg
     2701 caatgaacaa gttcattgaa aagatagcca tggcaaaaat cgaaggctca ttcattaaag
     2761 tcatcaatca cattgaaaaa aacgacctta ttatctttga tgacttcgga ttgcaaccgc
     2821 ttgataacaa ctcccgtttg gctctgctgc agatactgga agacttttat gaacggaaat
     2881 cagttatcat tacatcccaa ctgcctgtta aaaactggta cgattatatc ggagaaccta
     2941 ctctggccga tgcaataatg gaccgaatta ccagtaactc ggtaagggtt gagatattgg
     3001 gcgaatcaat gagaagaaaa cagaaaaaaa attaatactt ttatgtcaag tttacagctc
     3061 tttcttggtg gctcatttgt gagtgataca gtggcctatt tgatgtgata tattcattgt
     3121 ttattctcac agacaaagtc tgtttgtagt cgcaaaaaac acaagactaa tttttgtgca
     3181 aaaaagtttt ggaattaaaa ttaattattt aaaatctgga tagaagcata cttatttata
     3241 tttttggaaa acgactgaat aaaataaata gtatttcctt caatccattt aataccttcc
     3301 ggaacccaat cgctaggata aaaatcaaat aataatttta gcgaaccatt tgagcaattc
     3361 cataattgaa ttatattcgg gaatgggtca taattaaata ttcctgatgc atttataaaa
     3421 tattttgaag atggtgataa ttttggttct ccaaacattt cgtatttttg tccagtactg
     3481 tcatttataa gaatacactt tccaatttca taataagagg ctgtaaacaa atgaaaatta
     3541 atgttattaa aacttcccat ataactaaaa cgcacgtagt cagtcttttc ttcatccgaa
     3601 ctatctttca actcaatata agcattgttt tgtaacttaa taaatatact tttattctgt
     3661 tttatgattg atttaagttt tacaaaactt attgatctgt catcttttgt tgaaaaatat
     3721 tgtttttttg aaatagactt aaaatgtact ttggtagtat catcaatagt aattgtagta
     3781 tctaattgtt tgtttatttg ggaataagaa atattagtag taaagaagaa gaatattaaa
     3841 catattttat acatacatta tttattttta ataatattat cactactgtc ccgttaaaaa
     3901 acactactag tgaacaggtt
//
LOCUS       DAOPYB010000245         3915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_30558_length_3915_cov_10.709585, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000245 DAOPYB010000000
VERSION     DAOPYB010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30558_length_3915_cov_10.709585"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKH58_12420"
     CDS             <1..504
                     /locus_tag="PKH58_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNX89877.1"
                     /translation="YLSEAKKMGFDVVIHGDQFSADCGTLSAEHKAISIDHLEYADKR
                     EIEILAKSDVIPVVLPGASIGLGERFAPARKLLDAGCSLVIASDWNPGSAPMGNLLVQ
                     AAILGAYEKLSMAETWAALTCRAARALKRNDIGILKKGNKADFIVFETDDFREILYRQ
                     GQIKPAP"
     gene            584..2326
                     /gene="ftcD"
                     /locus_tag="PKH58_12425"
     CDS             584..2326
                     /gene="ftcD"
                     /locus_tag="PKH58_12425"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNX89878.1"
                     /translation="MKNNQPSQTPPAGGRGAALIECVPNFSEGNNMQVINQITDEIKS
                     VDGVKLLDVDPGKATNRTVITFVGEPNAVCEAAFRAVKKAAELIDMSKHRGEHPRFGA
                     TDVLPLIPIKNITMDETVEFARKLGKRIGDELGVPGYYYEFACTEEKRRNLAACRAGE
                     YEGLPKKFTNPEWKPDFGPAEFNETVRKTGAVALSARNLLIAYNVNLNTTSTRWANSI
                     AFDIREKGRIKREGNPLTGKIVKDADGNLVYTPGLLKGVKGIGWYIEEYGIAQLSFNI
                     TDTTAVPLHQVFELACERAAVRGIRVTGSELVGMVPLQIMLETGRYFLKKQHRSTGIS
                     DEEIIKIAVKSLGLDDLAPFDPKKKIIEYQLEDHSEKKLVDMTITEFANETASESPAP
                     GGGSVSACMGALGAALGTMVANLSAHKKGWDDRWEEFSDWAENGKNYSERLLSLVDQD
                     TQAFNGIMNAFGLPKNTEEEKAIRTEAIQSATKQAMEIPFQVMLTSYQSMEVMQAMAE
                     TGNPNSVTDAGVGALAARSAVLGAALNVRVNAGGLNDKDYVDKMLLEVNELVEKTIEK
                     ENEILQVVNEKIAK"
     gene            2385..3755
                     /gene="radA"
                     /locus_tag="PKH58_12430"
     CDS             2385..3755
                     /gene="radA"
                     /locus_tag="PKH58_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNX89879.1"
                     /translation="MAKTKSVYVCQNCGVSSPKWIGRCPSCGEWNSFVEEVVAKEPVI
                     KTQIAGFETITQKPALITDVETTIESRIDTNNQELNRVLGGGLVNGSLVLIGGEPGIG
                     KSTLVLQVVLNLQDKRTLYVSGEESVSQLKLRAERLKFNNPDCYIVSETSLEQIFVHV
                     QNVKPDLLIVDSIQTITTENIESSPGSVSQVRECAAALLKFCKTSGVPVLLIGHINKE
                     GSIAGPKVLEHIVDTVLQFEGDQHYMYRILRPIKNRFGSTSELGIFEMQQSGLREVSN
                     PSELLLSQNHEGLSGVAIASAIEGVRPFLIEVQSLVSSAAYGIPQRSSTGFDLRRLNM
                     LLAVLEKRAGFKLSQKDVFMNIAGGIKVNDPAIDLAVISAILSSSMDIALEPHICLAG
                     EVGLSGEIRPINRIEQRIHEAEKLGFKKIIIPTINLKSLDVKRINIELIQVRKVEEAF
                     RTLFKK"
BASE COUNT         1203 a          759 c          960 g          993 t
ORIGIN      
        1 tatctttcgg aagcgaagaa aatgggcttt gatgttgtca ttcacggcga ccagttttct
       61 gccgattgtg ggacattgtc ggcagaacat aaagctattt ctatcgatca tctggaatat
      121 gcggataaac gtgaaattga aattttagca aaaagcgatg tcattccagt tgtgttgccg
      181 ggtgcttcta ttggattagg cgaacggttt gctccggccc gaaagctgct cgatgccggt
      241 tgttcgctgg tcattgcgtc cgactggaac cccggcagcg caccgatggg caatttactg
      301 gtccaggcag ctattcttgg agcctatgag aaactctcaa tggcagaaac ctgggctgcg
      361 ctgacatgcc gggctgcaag agctttgaag cgaaatgata tcggaatatt gaaaaaagga
      421 aataaagccg attttatcgt gtttgaaacg gatgatttta gggaaatttt gtaccggcag
      481 ggacaaatta aaccggcccc ttagccccct aaagagggaa tcagagagca ttaatcaatg
      541 attttgtaaa tcaaaaacaa attaaataat agtaaaaaca aatatgaaaa acaatcaacc
      601 ttcacaaact ccccctgcag ggggtcgggg ggctgctctt atagaatgcg ttcccaattt
      661 cagcgaaggg aacaatatgc aagtaattaa tcagataacc gacgaaataa aatcagttga
      721 tggtgtaaaa ctacttgatg ttgatccggg aaaggcaacc aaccgcaccg tgataacttt
      781 tgttggcgaa ccaaatgccg tgtgtgaagc ggcattccgg gcggtgaaaa aagcggcgga
      841 actgattgac atgagcaaac atcgcggtga acatccccgt tttggggcaa cggatgttct
      901 accgcttatt ccaattaaga atatcacgat ggatgaaacg gtggaatttg cccgtaaatt
      961 agggaaacgc atcggtgatg aacttggtgt tccgggctat tattacgaat ttgcctgcac
     1021 agaggaaaaa cgccgtaacc tggcagcttg tcgtgcggga gaatacgagg gattgccgaa
     1081 gaagtttaca aatccggaat ggaaacccga ttttggcccc gcagaattta acgaaactgt
     1141 acgaaaaacc ggtgccgtag ctctcagtgc acgaaatttg ctgattgcct acaacgtaaa
     1201 cctcaacacc acctccaccc gctgggcaaa cagcattgct ttcgatatcc gtgagaaagg
     1261 tagaattaag agggaaggaa atccgcttac cggaaaaata gtaaaagatg ccgacggtaa
     1321 tctggtttac acacccggtt tgctcaaagg agtgaaagga attggctggt acatagagga
     1381 atacggcatc gctcaacttt cgttcaatat tactgataca acagctgtac cgctgcatca
     1441 ggtttttgaa ctcgcctgcg aacgggctgc tgtacgtgga attcgggtta ccggttcgga
     1501 actggttgga atggttcctt tgcagattat gcttgaaacc ggacgttatt ttctgaaaaa
     1561 acagcatcgt tctacaggaa tttcggatga agagattatt aaaattgccg taaaatcact
     1621 cggactggac gatttggcac cttttgatcc gaaaaagaaa atcattgaat atcagctcga
     1681 agatcattct gaaaagaaac tggtggatat gaccattact gaatttgcca acgaaacagc
     1741 atccgaaagt cccgcacctg gtggtggttc tgtttcggcc tgtatgggag cgttgggtgc
     1801 tgcactcgga acgatggtgg ccaatctgtc ggcacacaaa aaaggatggg atgaccgctg
     1861 ggaagaattt tccgattggg ctgaaaatgg gaaaaactat tctgaaaggt tattatcatt
     1921 ggtagatcaa gatacgcagg catttaacgg aataatgaat gcttttggac tacccaaaaa
     1981 tacggaagaa gaaaaagcaa tacgaaccga agctatccag tctgccacaa aacaggcgat
     2041 ggaaattccg tttcaggtga tgctgaccag ctatcagtcg atggaagtaa tgcaggccat
     2101 ggcggaaaca ggcaatccaa actccgtgac cgatgccgga gttggagcac tggctgcccg
     2161 aagtgccgtt ttgggagcag ctcttaacgt cagggtaaat gccggggggt taaatgataa
     2221 agattatgtg gacaaaatgt tgctggaggt aaatgagttg gtagaaaaaa ccatcgaaaa
     2281 ggaaaatgag atattgcagg tggtaaatga gaaaatagct aaataaaatc ccgacctgaa
     2341 accggaattt actacatttg tatccataaa gaaaaattga actcatggca aaaaccaaaa
     2401 gcgtttatgt ttgtcagaat tgcggagtaa gttcgccaaa atggattgga cgttgccctt
     2461 cctgtgggga atggaactcg tttgtggaag aagttgtggc taaagaacca gtaataaaaa
     2521 ctcagattgc cggttttgaa acaattaccc agaagcctgc actgattaca gatgttgaaa
     2581 caaccatcga atcacgcatt gatacaaata accaggaact caacagggtg ctgggaggtg
     2641 gtttggttaa tggatcactc gtgttgattg gaggagagcc cggaattggc aaatcgactt
     2701 tggttctaca ggtggttttg aatctgcaag acaaacgtac actgtatgtt tccggtgaag
     2761 aaagcgtcag ccagcttaaa ctacgtgctg aacggttgaa attcaataat ccggattgtt
     2821 atattgtcag tgaaacctct ctggagcaga tatttgtaca tgtgcaaaac gtaaaaccgg
     2881 atttgctgat tgtcgattcc attcagacca ttactactga aaatatcgaa tcatcacccg
     2941 gaagcgtatc tcaggttcgt gaatgtgctg ctgcgctgct taaattctgc aaaacatccg
     3001 gagttccggt acttcttatc ggtcatatca acaaagaagg aagtattgcg ggtccaaaag
     3061 tacttgaaca catcgttgac accgtgttac aatttgaagg tgatcagcat tatatgtacc
     3121 gcattctccg tcctatcaaa aacagatttg gaagtacttc agaattagga atttttgaaa
     3181 tgcagcaaag cggattgcgt gaagtaagta atccatcaga actattactt tcacaaaatc
     3241 acgaaggact gagtggtgtt gcaattgctt cggccatcga aggcgtccgg ccgtttctga
     3301 tagaagttca gtcgctggta agctcagctg cctatggcat tccgcagcgt tccagtaccg
     3361 gatttgatct tcgccgtcta aatatgctgc ttgccgtttt ggaaaaacgg gcagggttta
     3421 aactatcgca aaaggatgta tttatgaata tagccggtgg aataaaggtg aatgacccgg
     3481 ccatcgacct ggcggtaatt tcggctattc tctcatcgag catggatata gcccttgaac
     3541 ctcatatttg tctggcaggt gaggtgggac tttcaggtga aattcgtcca ataaaccgga
     3601 tagaacaacg aattcatgaa gctgagaaac ttggatttaa gaaaataata attcctacaa
     3661 taaatctcaa aagtcttgat gtaaaaagaa ttaatatcga attgatacag gttcgaaaag
     3721 tagaagaagc attcaggact ttgttcaaaa aatgacacga tgtaaaagat gttttatcaa
     3781 aagaagaaaa atttccggaa ttattttgtt attatcaaaa aagaccttat ctttgcatcc
     3841 gctttttgga cgaagtgtag gggcgtttag ctcagctggt tcagagcatc tgccttacaa
     3901 gcagagggtc ggcgg
//
LOCUS       DAOPYB010000246         3901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_30723_length_3901_cov_9.117005, whole genome shotgun sequence.
ACCESSION   DAOPYB010000246 DAOPYB010000000
VERSION     DAOPYB010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30723_length_3901_cov_9.117005"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1244
                     /locus_tag="PKH58_12435"
     CDS             18..1244
                     /locus_tag="PKH58_12435"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNX89880.1"
                     /translation="MHKIKVQNPVVELDGDEMTRIIWKFIKEQLILPYLDLDIRYYDL
                     GIESRDATDDRVTIEAAEAINQYHVGIKCATITPDEARVQEFNLRQMWKSPNGTIRNI
                     VGGTVFREPIIMSNIPRYVQGWTQPIIIGRHAFGDQYKATDKVIKGKGKLKLTFTNEN
                     GEVQEWEVYDFKGDGVAMAMYNTDESIYGFARSSFQMALTKHYPLFMSTKNTILKAYD
                     GRFKDIFQDVYENEFRTEFEKEGLTYEHRLIDDMVASAMKWNGGFVWACKNYDGDVQS
                     DTVAQGFGSLGLMSSVLVTPDGKTVEAEAAHGTVTRHYRQHQQGKETSTNPIASIFAW
                     TRGLEHRGKLDNNQELIDFCQKLEQVCIETVEGGEMTKDLAMLVYGENVTKEHYLNTQ
                     NFLAALKRNLDKKLNG"
     gene            complement(1318..2025)
                     /locus_tag="PKH58_12440"
     CDS             complement(1318..2025)
                     /locus_tag="PKH58_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX89881.1"
                     /translation="MKKLNVIVVLLLSASLILSGCKSLNNAAKGGMIGAGTGAAVGAG
                     LGVLIGKDGKSAAVGAAIGTAVGTTAGVIIGKKMDKAAAETAAQVENAKVETIEDVNG
                     LKAVKVTFDSGILFEFNKSNLNDASKTALTKFAKILVENPTMDISVLGHTDNIGTLEA
                     NKKVSLARATAVADYLVTNGAARSQMKTVEGRDYSMPVATNDTEAGRALNRRVEVYMY
                     ASEKMIDDAQKAASTSK"
     gene            complement(2079..2151)
                     /locus_tag="PKH58_12445"
     tRNA            complement(2079..2151)
                     /locus_tag="PKH58_12445"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2116..2118),aa:Lys,seq:ctt)
     gene            complement(2380..3897)
                     /locus_tag="PKH58_12450"
     CDS             complement(2380..3897)
                     /locus_tag="PKH58_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX89882.1"
                     /translation="MFHKKVAENAGIESDRISLIRILRKSIDARTTFPKINVAVEVFW
                     DENPPEKYEVHIDYGYVGDKPTVMVIGAGPAGLFAALKLIRLGIKPVLIERGKEARQR
                     KLDIAQLNRNKSINHESNYCFGEGGAGTFSDGKLYTRSKKKGSIKDVLDTFYFHGADE
                     HILYEAHPHIGSDNLPLVVENMRKTILTCGGEFYFERKLEELIVENDRITGAKISGGE
                     VLKADALILATGHSAHDIYEMLYRQGVELESKGFAMGVRVEHPQSLIDNIQYHTVKKN
                     DFLPAASYALVEQVNGRGVYSFCMCPGGHIVPAGTIEESIVVNGMSASRRNSPYANSG
                     IVVEIHPDDIPQEFQQYGVLAGLKFQQYVENLAYRNNGGKGFTAPAQRLEDFVKGKLS
                     ADLPECSYLPGIVSSPLHFWLPDMISGRLREAFKKFDRKMRGYLTNKAVVVGVETRSS
                     SPVRIPRDPETLQHIRIKGLFPAGEGSGYSGGITSSAIDGENCAQKASEYIRNIK"
BASE COUNT         1157 a          835 c          763 g         1146 t
ORIGIN      
        1 aaaaacattt aggaaatatg cataaaataa aagtacaaaa ccctgttgtg gagcttgacg
       61 gtgatgaaat gacccgcata atctggaaat ttataaaaga gcaattaatt ctgccctatc
      121 tggatcttga tatcagatat tacgatttgg gaattgagag ccgtgatgca acagacgatc
      181 gtgttaccat tgaagctgct gaagctatca atcaatatca tgtgggtatt aaatgtgcca
      241 ccatcacacc cgacgaagca cgtgttcagg aatttaacct tagacagatg tggaaatcgc
      301 caaacggtac gattcgtaac atcgtagggg gtactgtttt tcgcgaaccg atcatcatga
      361 gcaatattcc ccgctacgta cagggttgga cccaaccaat catcatcggg cgtcatgctt
      421 tcggtgatca gtataaagca accgacaagg tgataaaagg taaaggcaaa ctaaaattaa
      481 cttttacaaa tgaaaacgga gaagttcagg aatgggaagt gtatgatttc aaaggcgatg
      541 gagttgcaat ggcaatgtac aatacagacg aatctattta cggctttgct cgttcgtcgt
      601 tccagatggc gcttacaaaa cattatccgc tttttatgtc caccaaaaat accattctga
      661 aagcatacga cggacgtttt aaagacattt ttcaggatgt ttacgaaaat gaattccgga
      721 ctgaatttga aaaagaaggc ctcacttacg aacatcggct gattgacgac atggtggctt
      781 cagccatgaa atggaacggc ggatttgtgt gggcctgcaa aaactatgat ggcgatgtac
      841 aatcagatac tgtagctcag ggatttggat cgctgggact aatgtcatct gttcttgtta
      901 ctcctgatgg aaaaaccgtt gaagccgaag ctgctcatgg aactgtcacg cgccattatc
      961 gtcagcatca gcagggaaaa gaaacctcta caaatcccat tgcttccatt tttgcttgga
     1021 caagaggact tgagcatcgg ggtaaactcg acaacaatca ggaactgata gatttctgtc
     1081 agaagcttga gcaagtatgc attgaaactg ttgaaggcgg cgaaatgacc aaagacttag
     1141 ccatgctcgt atatggagaa aatgttacta aagaacatta tctcaataca cagaattttc
     1201 tagctgcact taaacgaaat cttgataaaa aacttaacgg ataaatttat gcccatatcc
     1261 ataaaaaaag gctttcggaa aaaatccgaa agcctttttt attttacttt tttctaatta
     1321 tttgctggta ctagcggctt tttgagcatc atcaatcatt ttttcgctgg catacatata
     1381 aacttcaacc ctgcgattaa gagctcgtcc ggcttctgta tcatttgtag ctacaggcat
     1441 agaataatca cggccttcga cggttttcat ctgagagcga gcagctccgt tagttacaag
     1501 ataatcagca acagctgttg cccgtgcaag tgagaccttt ttatttgctt cgagagttcc
     1561 gatattatcg gtatgtccga gaacagaaat atccatggtc ggattttcaa caagaatttt
     1621 ggcaaatttt gtaagtgcgg tttttgaagc gtcgttcaga ttacttttat tgaattcgaa
     1681 aagaattccg gagtcaaagg taacttttac cgctttcagg ccatttacgt cttcaatggt
     1741 ttcgacctta gcgttttcta cctgagcagc agtttcagca gcagctttat ccattttttt
     1801 tccgataatt acaccggcag ttgtacctac agcagtacca attgcagcac caactgcggc
     1861 gctttttcca tctttcccta tcaatactcc taatccggca cctaccgctg cacctgtacc
     1921 ggcaccaatc ataccacctt ttgcggcatt attcagggat ttacatcccg aaaggattaa
     1981 acttgcacta agtaataaaa caacaatgac atttagtttt ttcattttca attaaaatta
     2041 gtttgttaat aaaatttatt taataatttt tctttttgtg accccggagg ggctcgaacc
     2101 ctcgacccat tgattaagag tcaattgctc taccagctga gctacggagt cttcaaagaa
     2161 ttctacttgt ttgtaacgga taataatcaa agttgttcaa acttgcagaa acaatcaaaa
     2221 tttgatttac caaacagttt acaaaaactg tgcaattaaa tcttgagaga aattatttta
     2281 atatttttta taaaaaacca ctaaaatcat tgtgtttcga tggagcaaag gtaataattt
     2341 tttttcaaaa attcgtatac tgttaaaaaa aacatcggat tattttatat tccggatata
     2401 ttccgatgct ttttgggcgc aattctcacc atcaatagcc gatgaggtaa taccaccgga
     2461 gtacccagat ccttctcctg caggaaaaag tccttttatc ctgatatgtt gcagtgtttc
     2521 gggatcgcgg ggaattcgca ccggtgacga ggaacgtgtt tccactccta ctaccacagc
     2581 tttattggtc aggtaacccc gcatcttacg gtcaaacttt ttaaatgctt cgcgcaaccg
     2641 accgctgatc atatcgggca gccaaaaatg caatggtgac gatacgattc cgggtaaata
     2701 cgagcattca ggaagatcag cagagagttt acctttcaca aaatcttcca aacgttgtgc
     2761 tggtgccgta aacccttttc ctccgttatt tcgataagcc agattttcca catattgttg
     2821 aaatttaagt ccggcaagaa caccgtattg ctgaaattcc tgcggaatat catcgggatg
     2881 aatttcgaca acgatgccag aattggcgta gggagaatta cgccttgaag ccgacatccc
     2941 gttgacaaca atgctttctt cgatcgtgcc tgccggaaca atatgccctc cgggacacat
     3001 acaaaaagaa tacactccac gtccgttgac ttgttcgacc aatgcgtaag aagccgcagg
     3061 taaaaagtca tttttcttca ctgtgtgata ttgaatgttg tcaatcaacg actgagggtg
     3121 ttctaccctt acgcccatcg cgaaaccttt gctttccaat tccactccct gacggtaaag
     3181 catttcatag atatcgtgag cagagtgtcc ggtagctaaa atcaacgcat cagctttcag
     3241 gacctcacca ccggatattt ttgctccggt aatcctgtca ttttcaacaa tcaattcctc
     3301 cagttttctt tcgaaataaa attctccacc gcaagtcagg atggttttcc gcatattctc
     3361 aacaaccaga ggtaaattat cagaaccgat atgtggatga gcttcataga gtatatgttc
     3421 atcggctccg tgaaagtaaa aggtatcgag tacatcctta atactaccct tctttttcga
     3481 acgtgtataa agttttccat ccgagaatgt accagcacca ccttcaccaa agcagtagtt
     3541 tgactcgtga ttaatgcttt tatttcggtt aagttgagca atatccagtt tacgctgacg
     3601 cgcttctttt cctctttcaa tcaaaacagg ttttattccc agccgaatga gtttcagcgc
     3661 tgcaaaaagt ccggcgggac cggcaccaat aaccattacg gtgggtttat cacccacgta
     3721 tccataatca atatgaactt cgtatttttc aggtggattt tcgtcccaaa aaacttcaac
     3781 ggcaacattg atttttggaa aagtggtacg ggcatcgatc gattttctca gaatacgaat
     3841 cagtgatatt ctgtcggatt ctattcctgc attttctgct acttttttat gaaacagagc
     3901 t
//
LOCUS       DAOPYB010000247         3893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_30812_length_3893_cov_9.834289, whole genome shotgun sequence.
ACCESSION   DAOPYB010000247 DAOPYB010000000
VERSION     DAOPYB010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30812_length_3893_cov_9.834289"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..2816
                     /locus_tag="PKH58_12455"
     CDS             309..2816
                     /locus_tag="PKH58_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNX89883.1"
                     /translation="MSVNYTEQLNNVFIYSVQEAERLGNAYVGPEHLLLALIRVGNNR
                     ALDLLVSMNVDINNVKQKIESLVRTDMEPTGGEIPLLKSTEKIKKIMELEARSLSASS
                     ADTEHLLLAILKDKNNLAVDVLNSEKLTYESLQAYLKPVEPTTDQPKNNQASDDEDED
                     EDQFHKKSKSKQPGAKQSETPALDTFGSDITKAAMENRLDPIVGREREIERLAQILSR
                     RKKNNPVLIGDPGVGKSAIVEGLALRITQHKVSRVLFDKRVVALDMASIVAGTKYRGQ
                     FEERIKAILNELQKNPNIILFIDEIHTIVGAGGAPGSLDAANMLKPALARGEIQCVGA
                     TTLDEYRQSIEKDGALERRFQKVMVDPTTPDETLTILQNIQDRYEYHHNVRYTPEAIK
                     ACVRLTDRYITDRCFPDKAIDALDEAGSRVHIATVSVPAEIEQLEKQIEELKNQKNRV
                     IAEQKYEEAVPIRDKEKQCQYQLEDAIKRWEQESQQHPEIVDEEKVAEVVAMMSGIPV
                     QRIAQAEGLKLRQMKDVLKSRVIGQSDAVDKITKAIQRNRIGLKDPSKPIGSFLFLGP
                     TGVGKTHLARVLAEFMFDSPDALIRVDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTE
                     KVRRKPYSIVLLDEIEKAHPDVFNILLQVMDEGRLTDSLGRRIDFKNTIIIMTSNIGT
                     RQLKDFGKGVGFNTAEDSVVDAEFSRGVIQKALNKTFAPEFLNRIDDVVMFDQLSRDS
                     IKSIIDLELKGLFKRVTDLGYKLELSPEAKDFIAEKGYDVQFGARPLKRAIQRYLEDE
                     LADVVLSGEVAEGDTILMELNETKDGVKARIVKSIDQ"
     gene            complement(2896..3762)
                     /locus_tag="PKH58_12460"
     CDS             complement(2896..3762)
                     /locus_tag="PKH58_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNX89884.1"
                     /translation="METKGYLKSFYWKDYTMILGGLILYALGLTGFLLPNKIVTGGLA
                     GVTVLIKSATDIPLWLSYVSINGVLLIIALKIVGKNFVIKTVISVVLLTFLLRYGEMY
                     ITKPIIQADALLSSMIGAMLCGAGLGMVYSVNGSTGGTDIIGAVVTKYRHVSMGRVLL
                     LVDVLIVASSFFLFKSLEKIAIGLIVMAVMYYAVDLVISGMRQSVQFFIFTSKYEEVA
                     NHINSEIKRGCTIIDGMGWYSKKSQKIIVVLARKTESTSIFRLVKSIDQNAFVTQANV
                     VGVYGKGFDVLK"
BASE COUNT         1247 a          765 c          824 g         1057 t
ORIGIN      
        1 ttctttttaa accttttccg aattgagggt gcaaatatac gtcattttct ccaaatatca
       61 aaaaacccga aaaaatattt ctgtttattt ttctgctgaa attatatatg attttttaag
      121 gtttgtgcca ttttgtcagt tggtttgttg ttgtcttaat aaataattcg gacatctttt
      181 ccgaattata aatttaattt atttaggtat aatttttgaa gtatataaaa ttaaatgctc
      241 ctaatttcat aaatttgtgg catcatgtta cattatcaga acaaattatt ttattataaa
      301 acataaatat gtcagtgaac tacaccgaac aattaaataa tgtcttcata tacagcgtac
      361 aggaagctga acgactcgga aacgcctatg taggtcccga acacctgctt ttagctctaa
      421 tacgggtagg taacaaccgt gctttagacc tgctggtgag tatgaatgta gatatcaata
      481 atgtaaagca aaaaatagaa tcgctggtgc gtaccgatat ggaacctacc ggtggtgaaa
      541 tccccctgct taaaagtaca gaaaaaatta aaaaaatcat ggaactggaa gcccgttcgc
      601 tttctgcttc ttcagcagat acagaacatc tgcttctggc tattttgaag gataaaaata
      661 atcttgcagt cgatgtgctg aattccgaga aattaactta tgagagtttg caggcttact
      721 taaaacctgt agaaccgaca accgatcagc caaaaaataa tcaggcatcc gatgacgagg
      781 atgaagatga agatcagttc cacaaaaaat ccaaatcgaa acagcccgga gcaaaacaaa
      841 gcgagacccc agcactggat acctttggat cggatattac gaaagcagcc atggaaaacc
      901 gcctggatcc tattgtagga cgcgagcggg aaatagaacg gcttgcgcag attttgagcc
      961 ggagaaaaaa gaacaatcct gttctcattg gtgatccggg ggttggaaaa tcggccattg
     1021 ttgaagggct tgctttgcgg attactcagc ataaagtgtc gagggtactg tttgataaac
     1081 gggtggttgc cctggatatg gcatccattg ttgctggtac taaataccgc ggacagttcg
     1141 aagaacgtat taaagccatt ctcaacgagt tgcagaaaaa cccgaatatc atactattta
     1201 ttgacgaaat tcacaccata gttggtgccg gaggcgcacc gggttcgctc gatgctgcca
     1261 atatgctgaa gcctgctctt gccagaggcg aaatccaatg cgttggggca accactcttg
     1321 acgaatacag acaaagcatt gaaaaagacg gagcgctcga acgcagattc caaaaagtga
     1381 tggtcgatcc taccacaccg gatgaaacgc tgaccatact gcaaaatatt caggacagat
     1441 acgaatatca tcacaatgtg cgctatactc ctgaagcaat aaaagcatgt gtccgcctga
     1501 cagaccgcta tataactgat cgctgcttcc ccgataaggc tattgatgca ttggatgagg
     1561 caggttcaag ggtacatatt gctactgtgt ctgttcctgc agaaattgaa cagctggaaa
     1621 aacaaattga agaactgaag aatcaaaaaa acagggtcat tgccgaacag aaatacgaag
     1681 aggcagttcc cattcgcgat aaagaaaaac aatgtcagta tcagctcgaa gatgctatta
     1741 aacgttggga acaggaatca cagcaacatc cagagattgt ggatgaagag aaggttgccg
     1801 aggtggtagc aatgatgtcg ggcattccag tacaacgaat tgcccaggcc gaaggtctga
     1861 aactccgtca gatgaaggat gttctgaaaa gccgtgtaat tggacagagt gatgccgttg
     1921 ataaaatcac gaaagccata cagcgaaacc gcattggact gaaagatcca agcaagccga
     1981 ttggttcgtt cttgttctta ggccctacag gggtaggaaa aacacattta gcgcgcgttt
     2041 tggctgaatt tatgttcgat agccctgatg cccttattcg tgtggatatg agtgaatata
     2101 tggaaaaatt cagcgtttcc agattaatcg gagcgcctcc gggatatgtg ggctatgaag
     2161 agggaggaca actgaccgaa aaggttcgtc gcaaaccgta ttcaatcgta ttgctcgacg
     2221 agatcgaaaa agctcatccg gatgtgttta atatcctttt acaggtgatg gacgaagggc
     2281 gtttgaccga tagtctggga cgacggattg attttaaaaa tacgattata ataatgactt
     2341 ccaacatagg aacccgtcag ttgaaggatt ttggtaaggg agtgggattt aataccgcag
     2401 aagattctgt ggttgatgcg gaattctcac ggggtgtaat ccaaaaagct ctgaataaaa
     2461 cttttgcacc tgaattcttg aaccgtatcg atgatgtggt tatgttcgat cagcttagca
     2521 gggatagtat aaaatcgata atcgacctgg agctgaaggg gttattcaaa cgggtgacag
     2581 acctggggta taagctcgaa ctctctccgg aagccaaaga tttcattgcc gaaaaaggat
     2641 acgatgtaca attcggtgca cgtccgctta aacgtgccat tcaacgttat ctggaagatg
     2701 aattggcaga tgtggtactt tctggcgaag tagctgaagg agataccatt ttgatggaac
     2761 ttaatgaaac taaagatggt gtgaaagcaa gaattgtgaa gagtattgac caatagtata
     2821 atccgataca tcaataaaat atcccgtatg aactcgtcat gcgggatttt ttattatcac
     2881 aattatttga attgactatt tcaggacatc aaaaccttta ccgtaaacac cgactacatt
     2941 cgcctgtgtg acaaaagcat tctggtcgat gctttttaca agtcggaaaa tcgaggtcga
     3001 ttctgttttt cgtgccagaa cgacaataat tttttgcgat ttcttcgaat accagcccat
     3061 tccgtcgatt attgtacatc cgcgcttaat ctcagagttg atgtgattag ctacctcctc
     3121 gtatttggaa gtgaaaatga aaaactgcac ggattgtcgc ataccactga ttactaaatc
     3181 tactgcataa tacatcacag ccatcacaat taaaccgatg gctattttct ccagactttt
     3241 gaaaaggaaa aacgacgaag ccacaatcaa tacatctact agtaacagta cacgtcccat
     3301 acttacatgg cgatatttgg tgacaacagc accaataatg tcggtacctc ccgtacttcc
     3361 gtttacagaa tagaccattc ccagtccggc gccgcacaac atagcaccaa tcatggatga
     3421 caatagcgca tctgcctgga ttataggttt cgtaatgtac atctcgccgt atctgagcaa
     3481 aaaagtaagc aacaccacgc tgataactgt tttaattaca aaattcttac cgacaatttt
     3541 tagggcgatg atgagtagaa caccattaat agatacatag gataaccaaa gcggaatatc
     3601 agtggccgat ttgatgagta cagtaacacc agccaaacct ccggttacaa ttttatttgg
     3661 taataaaaaa cctgtcaaac ccagtgcata tagaattaat cctcccaaaa tcattgtata
     3721 atctttccaa tagaaagact tgagataccc ttttgtctcc ataaaacctc cagtcgtctg
     3781 ttattaatta atgtattttg aatgtattat taattacaca atttataaat ttgctgatta
     3841 ttcgtatccg taattgatag attaaacaat ttccccgtcc tgaaataatt ttt
//
LOCUS       DAOPYB010000248         3883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_30922_length_3883_cov_10.378788, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000248 DAOPYB010000000
VERSION     DAOPYB010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30922_length_3883_cov_10.378788"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..575)
                     /locus_tag="PKH58_12465"
     CDS             complement(57..575)
                     /locus_tag="PKH58_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89885.1"
                     /translation="MKKTAKALSLFLILFTCFGVTAAAQNKKADKDTRNWVYEIEPVS
                     VGTQGSYLIKVWSYSKKPIVAIEQAKKNAVHGIIFKGFTGIQGVPGQRPLTNNPNLEV
                     EQAEFFKNFFAEGGKYMKFVNITNDGSVAAEDRLKIGKEYKIGVVVSVNVAGLRKDLE
                     DAGILRGLSSGF"
     gene            complement(613..2352)
                     /locus_tag="PKH58_12470"
     CDS             complement(613..2352)
                     /locus_tag="PKH58_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89886.1"
                     /translation="MKRFSFLFTCVVLISGLYAQNPTGKVNTKYLRPSITMLFSQPKD
                     ADEEVVISKFRNLEVNSKFDNHRIDFPDMKPIEPLNLQKNALIEKYVRTASNPVIAKW
                     WGRDANGNFNYSLVAERGSYTATDADAIISRGSNTSRIEMIGEQLIDKSYILLYEITD
                     LYSMEEYYDRQDALNRKLKNYTPVKRTDEGYNCNYKVYAYKINFNDSVASEFYSRYWV
                     DSKNPDKQKVAAWADATFPVKLIASTGGSVRSAQSKEKSLNTKKKTMRELLEDIPANI
                     QNNAVFELGRKIEDFKLKVTVFKAYPVSAKLGTKEDLYIDQRFYVYEIEQDRNGNQMT
                     NRKGVVRVKTINENKQVATGSSLPSVFQQVDGGRIYPGMFMESKDDYGLILNIGKNGS
                     SNNAMEGFNIGADFRISRFLKKPGWHLGIDASLRSMKDVYAGDISTSTRYLINSNELC
                     SGSTYAVAVNLSKESYFTRKGNVYLRPQFGVGLQSYSFKEVGGYEIASDNKDYSWSSL
                     YVPFGIGIGWNITPMFSLEMKPGVFARFAASTDNKEKLIQSAALVIDNWGFGNIGKMN
                     VGTTNTICLRIRF"
     gene            2717..3139
                     /locus_tag="PKH58_12475"
     CDS             2717..3139
                     /locus_tag="PKH58_12475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89887.1"
                     /translation="MHQFIFKYGLVSDFVRYSPSDGWAPEAFSSDSYLSLTNLYSDNH
                     LPENWKTISVKTGISNTSDQKNIKLSVYPNPAIEWLNISGDFEENALIEILTLSGQQM
                     MALRSDSKGHSAVHISGLKPGIYIVRVNSATSKLVIGK"
     gene            complement(3528..>3883)
                     /locus_tag="PKH58_12480"
     CDS             complement(3528..>3883)
                     /locus_tag="PKH58_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNX89888.1"
                     /translation="SHDLSTDSLVTLMDFLLNEIQTKSPETKLYIQSLLPINESFGRY
                     KKLTGKTDQIPEINAKLKELANRRNIVFIDLFPLFTEDKTNILDKKITTDGLHLNEDG
                     YKIWVKKLKEYLADH"
BASE COUNT         1099 a          769 c          747 g         1268 t
ORIGIN      
        1 gataaaactg attatcagaa ataacttttt catagtcagt tagctgttag gagtgattaa
       61 aaaccgctgc ttaatccacg gagaattccg gcatcttcaa ggtccttgcg aagtccggcc
      121 acatttaccg aaaccaccac tccgattttg tattctttac cgattttcag ccggtcttcg
      181 gcggctacag aaccatcgtt ggtgatattc acaaacttca tgtatttacc gccttcggca
      241 aaaaagtttt taaagaattc ggcctgttcc acttccagat tgggattatt ggtgagcggt
      301 cgctgacccg gaacaccctg aatacctgtg aagcccttga agataattcc gtgaacggca
      361 ttcttttttg cctgttcgat ggctactata ggctttttgg agtaagacca caccttaatc
      421 aggtaggaac cctgtgtgcc tacactaacc ggttctattt cgtacaccca gtttcgggta
      481 tctttatcgg cttttttgtt ttgtgccgca gcagtcacac caaaacatgt gaataatatg
      541 agaaagaggc tgagcgcttt tgctgttttc ttcataatca atatttttta atggtatata
      601 attaacggtt aattagaatc tgatacgtaa acagatggta tttgtggtgc caacattcat
      661 ttttccgata tttccaaatc cccaattgtc gattaccaga gcagcagact gtataagttt
      721 ttccttatta tcagtcgaag ccgcaaacct tgcaaatacg ccgggtttca tctcgaggct
      781 gaacatcgga gtgatattcc agccgatgcc aataccgaac ggaacataca gggacgacca
      841 ggaataatcc ttattgtcgg atgcaatttc atatccaccc acttccttga acgaataact
      901 ctgtaaaccc actccgaatt gaggtctgag atatacattt ccctttcggg taaaatagga
      961 ttctttggat agattgacag cgacagcata ggtactgccc gaacataatt cattcgaatt
     1021 aatcagatag cgggttgaag tactgatgtc gccggcatat acatctttca ttgagcgcag
     1081 ggatgcatca atacccagat gccatcccgg ttttttcagg aaacgcgaaa ttcggaaatc
     1141 ggcacctata ttaaagcctt ccatggcatt gttggaggaa ccgtttttcc cgatattcag
     1201 aattaatcca taatcgtcct tgctttccat gaacattccg ggataaatcc gtccaccatc
     1261 gacctgctga aataccgaag gcagcgaaga accggtggca acctgcttgt tttcattgat
     1321 tgttttcact ctgaccactc cttttcggtt tgtcatctga ttgccgtttc tgtcctgttc
     1381 gatttcataa acatagaaac gctgatcgat gtacaaatcc tccttagtgc cgagctttgc
     1441 gctcacggga tatgccttga atacggtaac tttcagtttg aagtcttcaa tttttcttcc
     1501 cagttcaaaa acagcattat tctggatatt ggcaggtata tcttcaagca attcgcgcat
     1561 cgtttttttc tttgtattca gacttttttc tttcgactga gccgaacgca cagacccacc
     1621 cgtagatgcg atgagtttta ccggaaaagt cgcgtcagcc catgcagcaa ccttttgttt
     1681 gtcgggattt ttggaatcga cccagtatcg ggaataaaat tcgctggcaa ccgagtcatt
     1741 gaaatttatt ttataggcat acaccttata gttgcaatta tacccttcat cggtacgttt
     1801 caccggagta taatttttta gtttccggtt aagggcatcc tgccggtcgt aatattcttc
     1861 catcgaataa aggtcggtaa tttcgtagag gagtatgtaa gacttatcga tgagttgttc
     1921 gccaatcatt tcgatacggc tggtatttga acctctcgag atgattgcat cggcatctgt
     1981 cgcggtgtat gaaccccgtt cggccaccag tgaataattg aaattgccgt tggcatcgcg
     2041 tccccaccat ttggcaatga caggattgga tgctgtacgc acatattttt caatcaaagc
     2101 atttttctga agattcaggg gttcgatggg tttcatatcc gggaagtcaa ttctgtgatt
     2161 gtcgaattta ctgttgactt ccaggttacg gaatttggag ataaccactt cttcgtcggc
     2221 atcttttggc tgagaaaaca acatggttat ggaaggccgg agatatttgg tattaacttt
     2281 cccggttggg ttttgagcgt aaagaccgga aataagcacc acacatgtaa acaggaatga
     2341 aaatcgtttc atgaatataa attttggctt gccggagtac gacaagaagg ttagaaacaa
     2401 gagaacagac aggaatttca tgccggggaa ggtcagcccg cattcatatt gacctgagaa
     2461 ttatattgtt taaaaaattt ataaattcag ttatgatata ttcctattgt attttgattt
     2521 tattaatatt ccgaaagcaa aaatatacaa ttatttggat attggatgtt tttcatatag
     2581 taatgtgtaa aatatttatc cacattgaat cattatacat aaaatttgtt ctgggttatt
     2641 tgtaaaatcc ggttacagct taccaacctt atacttaatg ctacaaaaaa gatttgttaa
     2701 gttaacattt caacttatgc atcagtttat attcaagtat ggacttgttt ccgattttgt
     2761 aagatattcg ccttcggatg gctgggcacc ggaagcattc agttcagact catatctttc
     2821 gttgactaat ctttattcag acaatcattt acctgagaac tggaaaacca tatccgttaa
     2881 aaccggcatt agtaatacat ctgatcagaa aaacataaaa ctgagtgtat atccaaatcc
     2941 agctattgaa tggctcaata tcagcggtga ttttgaagaa aatgcactga tagaaatatt
     3001 gaccctgtca ggtcagcaaa tgatggcact cagatctgat tcaaaaggtc attcagctgt
     3061 tcatatttcc gggctgaagc cgggaattta tatcgtcagg gttaattctg ccacttcaaa
     3121 actggtgatt ggtaaataat tgagccataa aaagcatatt ttaatacctt ttttatactc
     3181 cggacgaatt tatttttctg ctatctttca gcatttgtat atttgtttgc caaaataaac
     3241 attggatgtt ttttggttta tcattacatg agttcgatat aaaaattcat ttaaccaaat
     3301 caaacacaat tgtctgcatt cccgtaatga attaatattc atagaaatat gtcattaaat
     3361 aaattaaata cccatttagg ggataaatat atcaattcca tatgggaaat gaatcgaaac
     3421 aaatacacgc tttctattga tatttaagtt ctaacggact atttcttatg tcgaacttac
     3481 gttaatctat ataagaaatg gtcgatttcc ttctgttttt gtggttttta atggtcagct
     3541 aaatactctt tcagtttttt tacccatatc ttatagccgt cctcattcag atgcaagccg
     3601 tctgtggtaa tttttttgtc caggatattt gttttatcct ctgtaaacaa cggaaacaaa
     3661 tcaatgaaga cgatatttct ccggttggcg agttctttca actttgcatt gatttcgggt
     3721 atctgatccg tttttcctgt aagtttcttg taacgcccaa acgactcgtt gattggcagc
     3781 aaactttgaa tataaagttt tgtttcaggc gatttggttt gaatttcatt aagaagaaaa
     3841 tccattaatg ttaccaggct gtcggtactc agatcgtgtg aaa
//
LOCUS       DAOPYB010000249         3874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_31025_length_3874_cov_9.317099, whole genome shotgun sequence.
ACCESSION   DAOPYB010000249 DAOPYB010000000
VERSION     DAOPYB010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31025_length_3874_cov_9.317099"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(571..1497)
                     /locus_tag="PKH58_12485"
     CDS             complement(571..1497)
                     /locus_tag="PKH58_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX89889.1"
                     /translation="MHFKFLILTSIVSLISINTNAQIFAVTANAAPENMKSAAKKVFP
                     LQKQNFPSVADTTNIYNDMLNDESDDLMENHPAGDIYNDIWSSERINPYKVPIESLPD
                     SVFINCADFVMPVSGGRVSSKFGPRRYRYHYGIDVAIQPGNEIRSVFSGKVRIIDYEP
                     GGYGNYVVVRHDNGLETVYAHLSFVKVKLNQTVKAGEELGLSGNTGRSTGPHLHFETR
                     YVGNAFNPATLIDFSEGKVFASHYMLTKRKNFYYQQSVRAQTLAKYYKVRKGDNLGRI
                     AARNGTTVTKLKRINGITKANKLKPGQKLRIR"
     gene            complement(1503..1964)
                     /gene="ndk"
                     /locus_tag="PKH58_12490"
     CDS             complement(1503..1964)
                     /gene="ndk"
                     /locus_tag="PKH58_12490"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNX89890.1"
                     /translation="MEKTLVIIKPCALQRALTGEVIKRFEQKGLRLAGIKMMQLDDRI
                     LTEHYAHLAERPFFQRIKDSMMITPVIVTCWEGKDAVKVVRAMTGSTNGREAVPGTIR
                     GDFSLSVQENIIHTSDSVETADVELARFFSDDEIFAYNYQSLPFLYANDEI"
     gene            complement(2064..>3874)
                     /gene="recG"
                     /locus_tag="PKH58_12495"
     CDS             complement(2064..>3874)
                     /gene="recG"
                     /locus_tag="PKH58_12495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNX89891.1"
                     /translation="FKGIRYVTEKYKTGVEYVIFGKPSLFNGRYNIVHPEMETVAQLP
                     PPEMLGLQPHYNTTEKMKNHFLNSKGIQKIMLPVIRQIQGGIPETLPAFLLKQFGLMN
                     LTESITNIHFPKNNELLSRARQRLKFEELFYIQLNILHQTKWREQHFEGFIFSRVGHN
                     FNTFYSQHLPFELTGAQKKVIREIRADMITGHQMNRLLQGDVGSGKTLVALMTMLIAI
                     DNGYQSCLMAPTEILATQHYATITAMLGNLGVQVSLLTGSTRQKDRVKIHEKLTNGEM
                     HIIIGTHALIEDTVRFNNLGLVIIDEQHRFGVAQRSKLWGKNHKPPHVLVMTATPIPR
                     TLAMTLYGDLSVSVIDELPPGRKPIQTYHYYETKRTALLSFITKQILAGRQIYVVYPL
                     IKESENMDMRNLEEGFEYLHQMLPQFSISMVHGKMKPAEKDAQMQLFISGETQLMVAT
                     TVIEVGVNVPNASVMIIESAQRFGLSQLHQLRGRVGRGADQSYCILLTGVKLSADSRK
                     RIEIMTRTNNGFEISEADLQLRGPGDLEGTQQSGLPFELKIANLAQDGQMLEIARHAA
                     SDILEKDPTLTNSENQIFVRQLKKLKTNTINWGAIS"
BASE COUNT          956 a          867 c          920 g         1131 t
ORIGIN      
        1 ttcgttcgtc aaaacctctt aactacgaag cgctatgata aatgttcctg cttgaaattc
       61 ttattcggca cattgcggtt tcatccgcat ggtgcatgga aaactgatgg ataatgccgt
      121 tttgataacg ggatgacaga gttccaactc tctcaattca ggccatttct ccatagaatt
      181 ggaattctgt cgtcccggtc agagagaaat tgcaattcca taacttggcg cgaaacatac
      241 aacaggtcgc tttgcggata atcataaatt attttgttcg agcggtaagg ataccgcttg
      301 tcctatgtat ggtaaagccc cctgattatc ttcctttggt cgctgaccgt tggttccccc
      361 tcaaggggga ctttgacagc attggtttca ttagaatttg cttaaataat tttcaaatgt
      421 aaacctaatt caggatgata caactctttc tctccctccc cggagggagg tctggctgct
      481 gaatcaaatg ggaaaacagg atttaaaaaa taatgaggat aacctgtaaa ggatatcctc
      541 atctgtatat atatatttat taataaagtc ttatcgtatt ctcaacttct ggccgggttt
      601 aagcttgttt gcttttgtaa tgccgttgat ccttttgagt ttagtaacgg tggttccgtt
      661 gcgggctgca attcttccga gattatcgcc tttgcgcact ttatagtatt tagccagtgt
      721 ctgtgctctg acagattgct gatagtagaa gttcttccgt ttggtaagca tgtaatgaga
      781 tgcaaacacc ttgccttcgg agaaatcaat cagagttgcc ggattgaaag cgttgcccac
      841 gtagcgggtt tcgaagtgca ggtgaggccc ggtcgaacgg ccggtgtttc cgctaagtcc
      901 gagttcttca ccggctttta cggtttgatt gagttttacc tttacaaaac tgaggtgagc
      961 ataaaccgtt tccagcccgt tgtcgtggcg taccaccaca tagttgccgt atccgcccgg
     1021 ttcgtaatcg atgatccgaa ccttgccgct gaataccgaa cgaatttcat tgcccggttg
     1081 gatggctacg tcaataccat aatgatagcg gtaccggcgc ggtccgaatt tggacgaaac
     1141 tctgcctcct gacaccggca tgacaaaatc cgcacagtta ataaaaacag aatcgggcag
     1201 actttcaatc ggtactttgt agggattgat ccgttcgctc gaccagatgt cgttgtagat
     1261 atcaccggca gggtggtttt ccatcaagtc gtccgattcg tcattcaaca tgtcgttgta
     1321 gatgttggtg gtatcggcta cggaggggaa attttgtttt tgtaaaggga agactttttt
     1381 agcagccgat ttcatgtttt cgggggctgc attggctgtt accgcaaaaa tttgcgcgtt
     1441 ggtattgatt gaaatcaggc ttactattga tgtaagaatt aagaatttaa aatgcattgg
     1501 cgttaaattt cgtcgttagc gtataaaaat ggcagcgact gatagttata agcaaaaatc
     1561 tcatcatcac taaaaaaacg tgcgagttcc acatcagctg tttcaacaga gtcggacgta
     1621 tgaattatat tctcctgcac acttaaacta aaatcaccgc gtatagttcc gggtactgct
     1681 tcacgaccgt tggttgaacc ggtcatggcg cgtacaactt tcacagcatc ctttccttcc
     1741 cagcaagtaa ctatcaccgg agtaatcatc atcgagtctt taatgcgttg gaagaaggga
     1801 cgttcggcca ggtgagcgta atgctccgtc aaaatcctgt catcgagctg catcattttg
     1861 atacctgcca ggcgaagtcc tttttgttcg aagcgtttga tcacttctcc ggttagggca
     1921 cgctgaaggg cacaaggctt gatgatgacg agggtttttt ccattaatat ttacgttgaa
     1981 cttttaattc aaaaataaaa tattctcaaa gataaagaaa atcaaattaa tagacaaagc
     2041 acatttgcat taaaaatatt tttttaacta atcgcgcccc agtttatcgt gtttgttttc
     2101 aattttttaa gttgtctgac gaaaatttga ttttccgaat ttgtaagcgt tggatctttc
     2161 tccaggatat cagaggcggc atgccgtgca atttcaagca tctgaccatc ctgagccaga
     2221 ttggcaattt tcagttcaaa aggcagtccg ctttgctggg tgccttccag atcgccgggc
     2281 ccgcgaagct gcaaatcggc ctccgatatc tcaaaaccat tgttggttcg ggtcataatt
     2341 tctatccgtt tgcgtgagtc tgccgagagt ttaaccccag tgagcaggat gcagtacgac
     2401 tgatcggcac cccggcccac acgaccgcgt aactgatgca actgcgacaa tccgaagcgc
     2461 tgggcgcttt cgataatcat caccgaagca ttcggcacat tcacaccaac ttcaataacg
     2521 gtggttgcca ccattaattg tgtctccccc gaaataaaaa gctgcatttg cgcgtctttc
     2581 tcggcgggtt tcatcttgcc gtgtaccatg cttatactaa actgaggcag catctgatgt
     2641 aggtattcaa aaccttcttc gagatttcgc atatccatgt tttccgattc ttttataagt
     2701 ggatacacca cgtaaatctg tcttccggca agtatctgtt tggtaatgaa actcaacagg
     2761 gcggtgcgtt ttgtttcgta gtaatgatat gtctggatag gtttacgtcc cggcggcagt
     2821 tcatcaatca ccgaaacgct cagatcgccg taaagcgtca tggcaagcgt gcgcggaatg
     2881 ggagtggctg tcatcaccag aacatgcggc ggtttatgat ttttccccca gagttttgag
     2941 cgctgcgcca caccaaaccg gtgctgctcg tcgataataa ccaaccccag attattgaag
     3001 cggacagtgt cctcaataag ggcatgtgtg ccgataatga tgtgcatttc tccgtttgtc
     3061 agcttctcgt gtatctttac acggtctttc tggcgggtag aaccggtgag gagcgaaacc
     3121 tgcacgccga ggttgcccag catggctgta atggtggcat aatgctgcgt ggccaggatc
     3181 tctgtcggtg ccatgaggca ggactgatag ccattgtcga tggcaataag catggtcatc
     3241 agagctacca gcgtctttcc gctgcccaca tcgccctgca gcagccggtt catctggtgc
     3301 cccgtaatca tgtcggcgcg gatttcacgg attacctttt tctgagctcc ggtaagttca
     3361 aagggcaggt gctgactata aaatgtgttg aagttatgtc cgacgcggga aaagataaac
     3421 ccttcaaaat gctgctcacg ccatttggtc tgatgaagta tgttgagctg aatgtagaaa
     3481 agctcttcga acttcagccg ctgacgggcg cggctgagca gttcgttgtt tttggggaaa
     3541 tgaatgttgg tgattgattc ggttaggttc atcagtccga attgtttcag taggaatgca
     3601 ggaagtgttt cgggaatgcc gccctgaatc tggcggatga ccggcaacat gattttctga
     3661 ataccttttg aattcagaaa atggtttttc attttctcgg tggtgttgta atgcggctgt
     3721 aaacccagca tctcgggcgg aggaagttgc gccacggtct ccatttcggg atgaacgatg
     3781 ttataccgac cgttgaagag cgatggcttt ccgaaaataa cgtattccac tccggtcttg
     3841 tatttctccg tcacataccg gatgcccttg aacc
//
LOCUS       DAOPYB010000250         3866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_31109_length_3866_cov_13.284702, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000250 DAOPYB010000000
VERSION     DAOPYB010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31109_length_3866_cov_13.284702"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..491)
                     /locus_tag="PKH58_12500"
     CDS             complement(84..491)
                     /locus_tag="PKH58_12500"
                     /inference="COORDINATES: protein motif:HMM:NF018425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNX89892.1"
                     /translation="MMKLLFRGNKFIEIVIYSIAIGIGMVLMLITRHSFWYLVPYILL
                     EIILLYFLITFRYVIVNQTVKVQYGFLNVKSIGIQNIHKIIIQDKQKGEPKITPHRIE
                     IQYDKAKKIEVSPKDTVGFVEIIERINPGIEII"
     gene            complement(533..1468)
                     /gene="nadA"
                     /locus_tag="PKH58_12505"
     CDS             complement(533..1468)
                     /gene="nadA"
                     /locus_tag="PKH58_12505"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNX89893.1"
                     /translation="MEKQKLTEEIAKLKKEKNAVIMAHYYQTADIQDIADVVGDSLAL
                     AQWAAKTDADVIVLCGVHFMGETAKILSPQKKVLIPDADAGCSLADSCPADKFEEFVK
                     AHPDHTVISYVNTTAAVKALTDVVVTSTNAKKIVDQFPHDAKLIFGPDKNLGNYINSV
                     TGRNMLLWDGACHVHEQFSLEKLIDLKKENPDAEIVAHPECKSVILKMADYVGSTQGL
                     LNYVTNSDRKKFLVVTESGILHQMTKANPQKTFIPVPPEFEGKTCMCNECKYMRMNTM
                     EKLYLCLRDEKPEILIEEELRSKAVKPILRMLEMS"
     gene            complement(1894..2709)
                     /locus_tag="PKH58_12510"
     CDS             complement(1894..2709)
                     /locus_tag="PKH58_12510"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX89894.1"
                     /translation="MKTILLTGGSAGIGLATAELLMNKGYRVYSGSRRGGGEVQKSPS
                     GGEIIPMILDVNDEESVKAAVEQIKGENDELYAVISNAGNGIAGDVEHTSTEEVKYQL
                     ETNFFGSVKVIQACLPVFRKQGYGKIIATTSVAAVIPIPFQAFYSAGKAALQIFVEAL
                     AMEVKPFGIQCCTILPGDTKTEFTSARKYTAASDNENSTYYRRMKTAVEKMEHDEQHG
                     MKPEAIAKAIVSQVERRKMKSRVIPGLMYKAICAVSDLLPNTLRLKLIDMVYS"
     gene            2834..3811
                     /locus_tag="PKH58_12515"
     CDS             2834..3811
                     /locus_tag="PKH58_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX89895.1"
                     /translation="MKILVTGANGLLGHHVVMELLERHEQVSIIVRSMENIYFDLQQV
                     HVFTGDFIDYETLKKAAFGCDAVIHSAAVTATNLLHYSDYEKVNVSGSANVLRVCNEL
                     GINRVVFISSSNTIGYGTSENQANERSPIQFPFTNSQYAQSKLAAEQLFFEASQLPDK
                     HIIIINSCFLIGGFDTKNSSGRLVKMGYRKPLLFVPKGGKNFVPASTVAQAACNALMM
                     GNNGEKYLVSGENLSFTDFFLLQKAVGHYKQLIIEVPDWILKLAGLAGDLLRKMGLKT
                     DLSTINIRQLLLREYYSGEKAKAELKYKSADLKEAISEAIKWLDKTEKV"
BASE COUNT         1086 a          800 c          693 g         1287 t
ORIGIN      
        1 acgataaact tttcaaaata gtaaaagctg aaaatttaaa gtctaaataa aaaaaagagg
       61 gtattgcagc cctctttttt ttattaaatt atttctatcc cgggatttat cctttcaata
      121 atttccacaa acccgactgt atctttgggt gaaacttcta tctttttagc cttatcatac
      181 tgaatttcta tccggtgcgg agtaattttt ggttctccct tttgtttgtc ctgaatgatt
      241 attttatgaa tattttgaat gccgattgat ttcacattca aaaatccata ttgtactttt
      301 actgtttgat taacgataac atacctgaaa gtaatcaaaa aatataatag gattatttcc
      361 agcaaaatat agggcactaa ataccaaaat gaatgccgcg taataagcat taaaaccatc
      421 ccgatgccga tagctattga ataaataact atttctataa atttatttcc tctaaaaagt
      481 aatttcatca tataaaattc gtgtaattcg tctcaatttg tgaaattcgt atttaagaca
      541 tttcaagcat ccttaaaata ggtttcacag cctttgagcg taattcttct tcaatcagta
      601 tttcgggttt ttcgtcgcgt aaacataaat agagtttctc cattgtattc atacgcatgt
      661 atttgcactc gttacacata catgttttac cttcaaattc gggtggcacg ggaataaatg
      721 ttttctgtgg attggctttg gtcatctgat gcagaatccc gctttctgtc actaccagaa
      781 atttctttct atccgaattg gtcacataat tcagcaaacc ctgtgttgaa cctacataat
      841 ctgccatttt caaaattaca cttttacatt cgggatgagc tacaatttca gcatcgggat
      901 tttccttttt caaatctatc agtttttcga gcgaaaactg ttcatgaaca tgacaggctc
      961 catcccaaag cagcatatta cgtcctgtga cgctgttgat gtaatttcca aggtttttat
     1021 ccggaccaaa aatcagcttt gcatcatgcg gaaactgatc cacaatcttt ttggcattgg
     1081 tagaagtcac caccacatcg gtgagcgctt ttacggcggc tgtggtattt acatacgaga
     1141 taacggtatg atcgggatgt gctttcacaa attcttcaaa tttatcggcc ggacaactgt
     1201 cggcaagcga acatccggca tccgcgtcgg gtatcaaaac ttttttctga ggcgaaagaa
     1261 tttttgctgt ttcgcccata aaatgcacac cgcacaatac tatcacatct gcatcggtct
     1321 tagctgccca ttgcgcaagc gccagactat cccccactac atccgctata tcctgtatat
     1381 cagctgtctg atagtaatgc gccataatta cggcattttt ttcctttttc agcttcgcaa
     1441 tttcttctgt taatttttgt ttttccatct tttctcagaa ttatcaaatc gaaattataa
     1501 taatattttt ttcctttttt aataaacctt ttggtgataa taatagcctg ttgatatggt
     1561 tgaaaaatta atggtaaaaa atttggaaaa tcaattatca aaaaaatcaa ccctctgttt
     1621 caacaaaatt attttctaaa atgttgctct tttacagttt tttatgaact ttatcaacaa
     1681 gctgttgaaa actgtcttgt tgaaattatt ttttcaacca aagactgata actccccgtt
     1741 tccgggttca aatctttctt taaagtttgt gctaacttat ttggtttttc agatatcatt
     1801 cctattacat gcaaggtttt gaatttccaa atttactttt tatatttctt tcaagagtta
     1861 tcagttagtt ttcaacaatc ggttaaacaa ctgttacgaa tacaccatat caatcagttt
     1921 aagccgtaag gtgtttggca ataaatcact tacagcgcat atagctttat acatcaatcc
     1981 gggaattact cgcgacttca tttttcttct ctccacctga ctgacaatgg ctttggcgat
     2041 ggcttcgggt ttcatcccgt gctgttcgtc gtgttccatc ttttcaacag ccgttttcat
     2101 cctccggtag tacgtcgagt tttcattatc cgatgctgct gtatatttgc gtgcgctggt
     2161 aaattcggtt tttgtatcgc cgggcagtat cgtgcagcac tgaatgccaa acggcttcac
     2221 ttccatcgcc agtgcttcca caaaaatctg caaagctgct tttccggcac tgtaaaatgc
     2281 ctgaaaggga attggaatta ctgccgctac cgaagtggtg gctatgattt tcccgtatcc
     2341 ctgtttcctg aacacaggca gacaagcctg gatgactttc accgaaccga aaaaattggt
     2401 ttccagctga tatttcactt cctccgtgct ggtgtgttca acgtcgcccg ctataccgtt
     2461 gcccgcgttg ctgataaccg cgtacagttc gtcgttttca ccctttattt gctcaacggc
     2521 ggcttttacg ctttcttcgt cattcacatc cagtatcata gggataattt cgccacccga
     2581 cggagatttt tgaacctctc ccccgccccg ccgcgaacca gaatataccc ggtaaccttt
     2641 gttcattaat agttcggctg ttgcaagtcc aatgcccgcg cttccgcctg taagtagtat
     2701 ggttttcata ttttgtagaa attcgtttta ataatgatta caaaattact tattttcagg
     2761 gaaataatcc gtatttttgc tttgagaatg tcaacatctt attagttggt tatcggcatt
     2821 ttattcactt tcaatgaaaa ttttagtaac aggagccaat ggtctgcttg gtcatcatgt
     2881 ggttatggag ttgctcgaac gccacgaaca ggtcagcatc attgtgcgca gcatggagaa
     2941 tatttacttt gatttgcagc aggttcatgt tttcacaggt gattttatcg attatgaaac
     3001 cttgaaaaaa gccgctttcg gctgcgatgc tgtcattcat tcagccgccg ttactgctac
     3061 caatttgctc cattattctg actacgaaaa ggtaaacgtg agtggatcgg ccaatgtgct
     3121 gcgtgtctgc aatgagcttg gcatcaaccg tgtcgttttt ataagctcct ccaatacaat
     3181 aggttacgga acatccgaaa atcaggcaaa tgagcgctct cccattcagt ttccattcac
     3241 caattcacag tatgcccaaa gcaaattagc ggctgagcag ttatttttcg aggcttcaca
     3301 actgccggat aaacatatta tcatcatcaa ctcctgcttt ctgattggcg gatttgacac
     3361 caaaaacagt tccggcaggt tggtaaaaat gggttaccga aaaccactac tttttgtgcc
     3421 aaaaggtgga aaaaattttg ttccggcaag tactgttgcc caagctgcct gtaatgcgct
     3481 aatgatggga aataatggtg aaaagtatct cgttagcggt gaaaatctca gtttcactga
     3541 tttttttctt ttacagaaag cggttggaca ttacaaacaa cttattattg aagtacctga
     3601 ctggatattg aaacttgcag gacttgcagg cgatttgcta agaaaaatgg gtctgaaaac
     3661 agatctgagc acgatcaata tccgtcagct tttactacgc gagtactatt ccggcgaaaa
     3721 agcaaaagcg gaattgaaat ataaatcagc agatttgaaa gaagcaatca gtgaggcaat
     3781 aaaatggctt gataaaactg aaaaggtata atttttgttg ttataatgtt gtcatttaat
     3841 cattctgaaa ggagaaaaaa ttatga
//
LOCUS       DAOPYB010000251         3859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_31189_length_3859_cov_15.329653, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000251 DAOPYB010000000
VERSION     DAOPYB010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31189_length_3859_cov_15.329653"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..540
                     /locus_tag="PKH58_12520"
     CDS             52..540
                     /locus_tag="PKH58_12520"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HNX89896.1"
                     /translation="MKRSFFTAFAVALIFGFISCGSVKPTAEQQQQQKLLREKIQSQH
                     YRFIATFVVPRGNFQPRYLTSEYDLKISKDTVEAYLPYFGVAYQAPTDLMGGGIKFLS
                     RKFDYSIKPGKKEGNWIVNIRTRDQLNEVILRLNIWENGNADLDVIETSRQSISFRGK
                     VE"
     gene            complement(604..1662)
                     /locus_tag="PKH58_12525"
     CDS             complement(604..1662)
                     /locus_tag="PKH58_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HNX89897.1"
                     /translation="MFEKQTFEAIELLKRMISHPSFSHEEKACADLIEHYIEEEGYTP
                     SRIGNNVWILGSNYDSSRPTLLLNSHIDTVKPVDGWNFDPFLPVENDGKLYGLGSNDA
                     GASVVSLLQVFFLLSKEKQTYNLIFAASAEEEISGSNGMESLLKELPKIDFAIVGEPT
                     NMQLAVAEKGLMVLDCIARGKAGHAARNEGENAIYKALADIEWFKNYQFPKVSEFLGE
                     VKMSVTQINAGTQHNVIPDKCSFVVDIRSNELYNNADILAVINSNVSCEVTARSTRLS
                     SSATPQNHPVVTKAKGMGMDIFGSPTLSDQALMPFASVKIGPGQSSRSHTANEYILTD
                     EIHEGIKKYFELLNGLSL"
     gene            complement(1681..2556)
                     /locus_tag="PKH58_12530"
     CDS             complement(1681..2556)
                     /locus_tag="PKH58_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX89898.1"
                     /translation="MKRFLFFTFIMSIFFSKGISQEKILLYPNGPAESNELKVEESWR
                     DKDFLLNISEPRMYAYPASKENNCGTAVLICPGGGYSGVSVIKEGEEFARWFNKLGVS
                     AFVLYYRMPNGHYEIPLKDAQTALSIIHTRAKEWKINKNKIGIMGFSAGGHLASTAGT
                     HFKTKAERPAFMLLAYPVVTMNKDLTHKGSRDNLLGKDPSDELVKLYSNELQVTKKTP
                     PTFMVHALDDGAVPIANSQNLLKSLQENKVPSKLVTYDQGGHGFGMRKKGIPVENWPQ
                     ELKAWLTERKLIKNN"
     gene            complement(2560..3390)
                     /gene="argB"
                     /locus_tag="PKH58_12535"
     CDS             complement(2560..3390)
                     /gene="argB"
                     /locus_tag="PKH58_12535"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNX89899.1"
                     /translation="MNDISDIFHSAKKSPKGDLRGQLTLIKVGGKIVEEPESLKQLLA
                     DFSEIPGHKVLVHGGGRSATALATQLGIESKMVNGRRVTDEEMLKVVTMVYGGLVNKK
                     IVAGLQALGVNALGLTGADLNYMQSEKRPVTEVDYGYVGDVKKVNADILADLISKDVV
                     PVLAPLTHDKNGNLLNTNADTIAGEAAKALAKHFDVTLMYCFEKKGVLMNEADDDSVI
                     ENINPATFQKLVAEGVIQGGMIPKLENSFEALKSGVKQVVITRADLIKMKNTCTTIKL
                     "
     gene            complement(3495..>3859)
                     /locus_tag="PKH58_12540"
     CDS             complement(3495..>3859)
                     /locus_tag="PKH58_12540"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNX89900.1"
                     /translation="VVGVESRGFMLGSILAYKLNAGFVLLRKKGKLPAETYSINYELE
                     YGTDELEIHKDAIEPNDVVLLHDDLLATGGSANAALQLLRKFGNNPIYVSFMIELVEL
                     NGISLLGEAKDIHSLVKF"
BASE COUNT         1018 a          875 c          763 g         1203 t
ORIGIN      
        1 tttttgttgt tataatgttg tcatttaatc attctgaaag gagaaaaaat tatgaaaaga
       61 tcatttttta cggcgtttgc tgttgccctc atcttcggat ttatttcttg cggcagtgta
      121 aaaccaactg ccgaacaaca gcagcagcaa aaactcctgc gcgaaaagat acaatctcag
      181 cattatcgtt ttattgccac ttttgtagtt ccaagaggta atttccagcc tcgctattta
      241 acctccgaat atgatcttaa aatttcgaaa gataccgtgg aagcttatct cccctacttt
      301 ggagtggctt atcaggctcc caccgattta atgggtggtg gaataaaatt cctcagccgc
      361 aaatttgatt acagcattaa acccgggaaa aaggaaggta actggattgt taatatccgt
      421 acccgcgatc aacttaatga ggttatttta aggctgaata tctgggaaaa tggcaatgcc
      481 gatctggatg tgattgaaac ttcccgccag tccatctctt tcaggggaaa agtggaataa
      541 aaagcatgaa aacaaaaaga gaagattttc ggtcttctct ttttgtttgt attatttccg
      601 gtcttataac gataaaccgt tcagcagttc aaagtatttt tttattcctt cgtgaatttc
      661 atcggttaga atatattcat tcgctgtatg tgagcgtgat gactgtcccg gacctatttt
      721 cacacttgcg aaaggcatca gtgcctgatc cgaaagcgtg ggggaaccga agatatccat
      781 tcccatccct ttggctttcg taaccacagg atgattttgc ggcgtcgctg acgaactcaa
      841 ccgggtggaa cgcgccgtta cctcacagct gacgtttgag ttgattacag ccagaatatc
      901 ggcgttattg taaagctcgt tgctgcggat atccaccaca aaactgcatt tgtcgggaat
      961 tacattgtgc tgtgttcccg cgtttatctg cgttacgctc attttcacct cacccagaaa
     1021 ttcagaaacc ttcgggaact gataattttt aaaccattct atgtctgcaa gcgctttgta
     1081 aatcgcgttt tctccttcgt tacgggctgc atgtccagcc ttgccccgcg cgatgcaatc
     1141 cagcaccatc agtccttttt ctgccactgc caactgcata ttggtaggct cacccacaat
     1201 ggcaaaatca atcttcggaa gttctttcag caaactttcc ataccgttac tgccagagat
     1261 ttcctcctct gccgatgccg cgaatatcag gttgtatgtc tgtttttctt tcgataacag
     1321 gaaaaaaacc tgcaacagcg aaaccaccga agcgcctgca tcgttgcttc ccagtccgta
     1381 tagttttcca tcgttttcca ccggcaaaaa aggatcgaaa ttccatccgt caaccggttt
     1441 tacggtatca atatgcgaat tgagcagcag agtgggtctc gaactgtcat agttacttcc
     1501 caaaatccag acattattac caatccgtga aggggtatac ccctcctctt cgatgtaatg
     1561 ctcaatcaga tcggcacatg ctttctcttc gtggctgaac gaaggatgtg aaatcattct
     1621 tttcagcagc tctatagctt cgaatgtttg tttttcaaac atgtgaaaac acgtttatta
     1681 tcaattattt ttgatcagtt ttctttctgt cagccaggct ttcagttcct gcggccagtt
     1741 ttcaaccggt attccttttt ttcgcattcc gaaaccgtgt ccaccctggt cgtaagttac
     1801 aagttttgac ggaaccttat tttcctgcag agatttcagc aggttttggc tgttagcaat
     1861 gggtacggcg ccatcatcga gcgcatgaac catgaaagtt ggtggtgttt ttttagtcac
     1921 ctgaagttcg tttgaataca gtttaaccaa ttcgtctgaa ggatctttcc caagtaaatt
     1981 atcccgtgaa cctttgtgag ttaggtcctt attcatggta acaaccggat aagccagtaa
     2041 catgaaagcc ggtcgttcag ctttagtctt gaaatgagtt cctgcggttg atgccagatg
     2101 acctccggct gaaaagccca tgataccgat tttatttttg ttgattttcc actctttagc
     2161 tcgtgtatga ataattgaaa gggctgtctg ggcatctttc agaggtattt cataatgtcc
     2221 gttaggcatc ctgtagtaaa gcacaaaagc agataccccc aatttgttga accatcgcgc
     2281 gaattcttca ccctctttta tgaccgaaac accactataa cccccacccg ggcaaatcag
     2341 aacagctgta ccacaattat tttcttttga agccggataa gcatacatgc ggggctcgct
     2401 gatattcagc agaaagtctt tatctctcca cgattcttct acctttagtt cattgctttc
     2461 agctggtccg ttcggataaa gcaggatttt ttcctgtgaa atgccttttg aaaaaaatat
     2521 actcattatg aaggtgaaaa ataaaaatcg tttcatactc taaagtttaa tggtggtgca
     2581 ggtatttttc atttttataa ggtcggcacg cgtgattact acttgtttta cgcccgattt
     2641 aagcgcttcg aacgagtttt ccagcttggg tatcataccc ccctgaataa cgccctcagc
     2701 gacgagtttt tggaacgtag cggggtttat attttcaatt acactgtcgt cgtcggcttc
     2761 attcatcaaa acgccttttt tttcgaaaca atacatcagc gtaacatcaa aatgctttgc
     2821 aagtgctttg gcagcttctc cggctatcgt gtcggcattt gtattcagta aattcccgtt
     2881 tttatcatga gtgagcggtg ccaatacagg tacaacgtcc tttgaaatta aatcagccag
     2941 aatatcggca ttcacttttt ttacatctcc aacgtatcca taatctacct cagtcacggg
     3001 acgtttttcg gattgcatat agttaagatc agcgccggta agccccagtg cattcacacc
     3061 gagagcctgc agtcctgcaa ctatcttttt atttaccagt ccgccgtaaa ccatggttac
     3121 aactttcagc atctcctcgt cggtaacacg tctgccgttt accattttgc tttctatacc
     3181 gagttgggta gccaaagcag tagccgaacg gccaccgccg tgtaccagca ctttgtgtcc
     3241 gggtatttct gaaaaatcag caaggagttg tttgagactt tcaggctctt caacaatttt
     3301 tccgccaacc ttgataaggg taagttggcc ccttaaatcc cccttggggg acttttttgc
     3361 tgaatggaaa atatcggaaa tatcgttcat aattggatag ttaagaaaag aaagatacca
     3421 acttcgtttt taaatgatta tattaggtga aagagaatct gtaagtcccg caagggggat
     3481 ttagggggct attattaaaa ttttaccagt gaatgaatat cctttgcttc accgagaagt
     3541 gaaatgccgt ttaactccac caattcaatc ataaaactca cgtagatagg attgttaccg
     3601 aatttgcgta aaagttgaag cgctgcattg gcacttccac ctgttgcaag caaatcgtca
     3661 tgtaacaata ctacatcatt cggttcgatg gcatctttat gtatttcgag ttcatcagtt
     3721 ccatattcta gttcataatt tatgctgtat gtttcagcag gtaacttgcc ttttttacga
     3781 agcagtacaa atcctgcatt aagcttataa gccagaatgc ttccaagcat aaaaccgcga
     3841 ctttccactc ctacaactt
//
LOCUS       DAOPYB010000252         3837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_31466_length_3837_cov_11.734532, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000252 DAOPYB010000000
VERSION     DAOPYB010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31466_length_3837_cov_11.734532"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKH58_12545"
     CDS             <1..360
                     /locus_tag="PKH58_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNX89901.1"
                     /translation="KQSMVRESIGISEDGNRIYAKTMQDSVVCYSAQKNLPKETWTTN
                     LAFGYEHNPSMLIEKDGIVFGSTKNGLIFALETLTGKVIWKHKVGNSLINTVLPIGNK
                     KLLFTAASGETGLIIWK"
     gene            386..1846
                     /locus_tag="PKH58_12550"
     CDS             386..1846
                     /locus_tag="PKH58_12550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNX89902.1"
                     /translation="MKTTINCFIPFKSKSQAEQTVKSLMETGLVNKIYLLAIDVVSDK
                     IDEHEIITVPSLFSSATMREIAKRADADFTLFYTKYTTLEMGMFALERMIHIMNDSSA
                     GMSYADHYQIVGDVKTSAPVIDYQLGSLRDDFDFGSVLLFKSDCFTEAATRLTTDYQF
                     AGWYDFRLKFSQKYSLVHINEFLYSEVEQDTRKSGEKMFDYVDPKNRGLQIEMEQACT
                     EHLKEIGGYLAPKFEKIEFNAGNFEFEASVVIPVRNRVRTIKDAIASVLSQKTNFKFN
                     LIVVDNHSNDGTTEAIREFSSDSRLIHVIPERQDLLIGGCWNLATHNEKCGKFTVQLD
                     SDDVYKDENTLQTIVNAFYEQNCAMVVGTYMITDFEMNTIPPGVIDHKEWTPENGRNN
                     ALRINGLGAPRAFYTPVLREVKVPNTIYGEDYALGLNFSRKYQIGRIYDVLYLCRRWE
                     GNSDAALDIVKSNANNLYKDRIRTWELQARIALLKK"
     gene            1960..2904
                     /locus_tag="PKH58_12555"
     CDS             1960..2904
                     /locus_tag="PKH58_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HNX89903.1"
                     /translation="MKDQILELFNQQLLEWDTAQHFYTALNEVKVKETEVNGCRYQIQ
                     FNPARIVSSGAKVDAKTIQERKCFLCSANRPTVQKGIDFNGHYTILINPFPIFPKHFT
                     IPEKEHTEQLILPRFGDMLDLAKAMENYVIFYNGPKCGASAPDHAHFQAGNKGFLPLE
                     TEWKNHITEKISDNLFAINYGFPAFVIKAEMTNKAEAVSLFGKIYNTLLVNNGETEPM
                     MNVLAWYETSTTGGEGTVVIVVIPRAKHRPDCYFAESDENILISPAAVDLGGVFITPL
                     EKDFEKITDKNVAEILGEVCLDKSTAEKLTAKLKELSL"
     gene            2901..>3837
                     /locus_tag="PKH58_12560"
     CDS             2901..>3837
                     /locus_tag="PKH58_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNX89904.1"
                     /translation="MNEPKVNVGILFSAEIGFELPSPYIVNGKEASGKQNVKCHDNKV
                     QWNGELYDELRFDPLNEKTDVFELKDVVIGINFHWERKENQRFLGALKFIVEDEKLTA
                     INVISVEDYLTSVISSEMSATASLELLKAHAVISRSWLLAKSLASQKNETNSPVPVSI
                     QEFKTEDGIDTYIRWWDREDHTNFDVCADDHCQRYQGITRASTGIVRQAIDATFGQVL
                     TYDNEICDARFSKCCGGVYEEFQYCWDNKKYAYLAKQRDSKTETALPDLRIETEAEKW
                     IRTSPAAFCNTNNKKVLSQVLNNYDQETTDFYRWKV"
BASE COUNT         1246 a          719 c          830 g         1042 t
ORIGIN      
        1 aaacaatcca tggtgcgtga aagtatcggc atttccgaag acggaaaccg catctatgca
       61 aaaaccatgc aggatagtgt tgtctgctat tctgcacaga aaaatctgcc gaaggaaacc
      121 tggacgacga acttagcatt tggttatgaa cacaatcctt ccatgcttat cgaaaaagat
      181 ggaattgtct ttggaagtac aaagaacgga cttatctttg cgttggaaac actgaccggg
      241 aaagttatat ggaaacacaa agttggaaat tcattaataa ataccgtttt accgataggc
      301 aataaaaaac tgttgtttac cgctgcaagc ggagaaactg gtcttataat ctggaaataa
      361 taaactcaat aatagaatta aatttatgaa aacaacgatt aattgcttca tcccttttaa
      421 aagcaaatcg caggctgaac aaaccgtaaa aagtttgatg gaaacaggat tagtgaacaa
      481 gatatatctg ctggccatag atgtcgtttc tgataaaatc gacgaacatg aaattattac
      541 agttccatcg ctgttttcaa gtgcaaccat gagagagatt gccaaacgtg ccgatgctga
      601 tttcacgttg ttctacacca aatatactac gctggaaatg ggcatgtttg cgcttgaacg
      661 tatgatacac atcatgaacg atagttcagc aggaatgtct tatgccgatc attaccagat
      721 tgttggcgat gttaaaacct ctgcgccggt tatcgattat caactaggaa gccttcggga
      781 tgactttgat ttcggatccg tgttgttgtt taagtctgat tgtttcacag aagcagccac
      841 ccgcctgaca actgattatc agtttgccgg atggtatgat ttccgcctga aattctctca
      901 aaaatattct ctcgttcaca tcaatgaatt tctgtattcc gaagtggaac aggacacacg
      961 aaaaagcggt gaaaagatgt ttgattatgt agatccgaaa aaccgcggtt tacaaattga
     1021 aatggaacag gcttgtaccg aacacctcaa agaaattggc ggttatctgg ctccgaaatt
     1081 cgaaaagatt gaattcaacg caggcaactt tgaatttgaa gcatcggttg tcatacctgt
     1141 gagaaatcgg gtacgtacca tcaaagatgc aattgcctcg gtacttagtc aaaagacaaa
     1201 ttttaaattc aatcttattg ttgtagataa tcattccaac gacggaacca ctgaagctat
     1261 tcgtgaattc tcatccgata gccgtcttat acatgttatt cctgaacgac aggatttgtt
     1321 gattggcggt tgctggaacc tcgctactca taacgaaaag tgtggtaaat ttaccgttca
     1381 actcgacagc gatgatgtat ataaagatga aaacacgctc caaacaattg taaacgcgtt
     1441 ctacgaacaa aactgtgcga tggtggttgg aacgtatatg attaccgact ttgaaatgaa
     1501 tacaattccg ccgggagtta tcgaccataa ggagtggacc ccagaaaatg gcagaaataa
     1561 tgcattgaga attaatggat tgggagcgcc gcgtgcattc tacactcctg ttttgcgcga
     1621 agtgaaagtt ccgaatacca tttatggtga agattacgcg ctcggactta atttctcccg
     1681 caaatatcag atcggacgta tttatgacgt tctttatcta tgtcgccgct gggaaggaaa
     1741 ttcagatgca gcactggata ttgtgaaatc aaatgccaac aatttatata aagacagaat
     1801 aagaacctgg gaattgcagg cacgtattgc attgttgaaa aaataaaagt ttcaatactg
     1861 ctgaaaatta attcagtccg gagatgaata attgcagcgc attctgcaat tattcatctt
     1921 caattactaa taacgaaaag gcattgttgc caaaagaata tgaaggatca gattttagaa
     1981 ttattcaacc agcaattatt ggagtgggat acagcacagc atttttatac agcgctgaat
     2041 gaagtaaagg tcaaggaaac cgaagttaat ggttgcaggt accagataca gttcaatccg
     2101 gcccgtattg tatcttccgg agcaaaagtg gacgccaaaa ccatacagga acgcaaatgt
     2161 tttctttgca gtgccaatag gccgacagta caaaaaggca ttgatttcaa cggtcattac
     2221 accattttga tcaatccatt tccgattttc ccgaaacatt tcaccattcc tgaaaaagaa
     2281 cataccgaac agctgatctt gccccgtttt ggcgatatgc tcgaccttgc taaggccatg
     2341 gaaaattatg ttatttttta taacggtcct aaatgtggcg cttctgcccc cgatcatgca
     2401 cattttcagg caggaaacaa aggatttctt cccctggaaa cagagtggaa aaatcatatc
     2461 acagaaaaaa tcagcgacaa tctttttgcg ataaattatg gttttccggc ttttgtgata
     2521 aaagcagaga tgacgaataa ggccgaagct gtttcacttt ttgggaaaat atataatacg
     2581 cttttagtaa ataatggtga aaccgaacca atgatgaacg ttctcgcctg gtacgaaact
     2641 tccactaccg gaggtgaagg tacagtagtg attgttgtca ttccacgtgc taaacaccgt
     2701 ccggattgtt attttgccga aagtgatgaa aatattctga taagtcccgc agccgtggat
     2761 ttgggtggtg tatttattac tcctctggaa aaagattttg aaaaaattac ggacaaaaat
     2821 gttgctgaaa ttttaggaga agtttgtttg gataaaagca cagcagaaaa attaacggca
     2881 aaattgaagg aattatcatt atgaatgaac caaaagtaaa tgtaggcata ctgttttccg
     2941 ctgaaatcgg gtttgaactg ccaagtccgt acattgtaaa cggaaaagaa gctagtggaa
     3001 agcaaaatgt aaaatgtcac gataataaag tgcaatggaa cggtgaactt tacgatgagc
     3061 ttcgttttga tccgttaaat gaaaaaaccg atgtttttga gctgaaagat gttgttatcg
     3121 gcatcaattt tcattgggaa agaaaagaaa atcaacgttt tctgggcgcg ttaaagttta
     3181 ttgttgaaga tgaaaaactg acagccatca acgtgatttc ggtggaagat tatctgacca
     3241 gcgtaatatc atctgagatg agtgccacag cttcactcga attgctgaaa gcacatgcgg
     3301 taatatcacg cagctggctg ctggcaaaaa gtctggcatc gcagaaaaat gaaacaaatt
     3361 caccggttcc tgtaagcatt caggagttca aaactgagga tgggatcgat acctacattc
     3421 gttggtggga tcgtgaagat cacaccaatt ttgatgtttg tgcggatgac cattgccagc
     3481 gttatcaggg cattacgcga gcctcaaccg ggattgtaag acaagcgatt gatgctactt
     3541 tcggtcaggt gctgacttat gacaatgaaa tttgcgatgc ccgtttttcc aaatgctgcg
     3601 gtggcgttta tgaagaattt cagtattgct gggacaataa aaaatatgct tatctggcaa
     3661 aacagcggga cagcaaaacg gaaacagcat tgcccgatct gcgcatcgaa acggaagccg
     3721 aaaaatggat acgtacatca ccggctgctt tttgcaatac aaacaataaa aaagtgcttt
     3781 cgcaggtact gaataattac gatcaggaaa caaccgattt ctaccgctgg aaagtgg
//
LOCUS       DAOPYB010000253         3831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_31524_length_3831_cov_11.626589, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000253 DAOPYB010000000
VERSION     DAOPYB010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31524_length_3831_cov_11.626589"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PKH58_12565"
     CDS             <1..460
                     /locus_tag="PKH58_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89905.1"
                     /translation="PFYEKYVFTDKPQQSDFDSNDNTNPLIFDMHLITGLHNNFRSFI
                     FETPDIDTFKNYFRERPMPIKKRKGITISELCYLFGKIESKQTGVSNFAEWMKNHIEG
                     NNYGSKKNELIDLAKNAKAKRNGFSLTAPQQKALENKDKIDKLFNRMHPD"
     gene            666..941
                     /locus_tag="PKH58_12570"
     CDS             666..941
                     /locus_tag="PKH58_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3853 family protein"
                     /protein_id="HNX89906.1"
                     /translation="MEKRIFELTVSEFIELLKQEQPVQVMPEQKTEPKMLYSIKELAK
                     FLQCSTVTAQKIKNSGKIPFSQTGRKVVFDGNAVMSALSVKMKGQRA"
     gene            1071..1331
                     /locus_tag="PKH58_12575"
     CDS             1071..1331
                     /locus_tag="PKH58_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89907.1"
                     /translation="MKADKITNETQVFFDYLQENVCTATMVSKETGIEQKNITRYKRG
                     LEKMYLLYVIEKKRCPITGFPAQWITTNPFMFPQNNKQLTLF"
     gene            1333..2856
                     /locus_tag="PKH58_12580"
     CDS             1333..2856
                     /locus_tag="PKH58_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNX89908.1"
                     /translation="METRQYKKWNKNPEVTQFSIDGKQPPSDSDAEDYLLGCLMSESG
                     AIDLVGSITSDVFYSEPNKAIFRACKRLQSKDSVISPISVNRELTADERAIFTEAPEL
                     YLYAITGKIGTTTDLENTAFHLKDVSIKREMVLSALQTITDIDTLDPLDNLTIGKDRL
                     VSIENSFYSDYSFDKYIVPRHLDFDRPEPILYRNEDEILHRKDLQFIEGQSGSKKTFL
                     VSAMIAGLIGDDLEKSMHFNTIKNEVKVLFIDTEQSIGNVQRVARRIHRMAGIDERSD
                     FDNLIVLGLRECTPVERLAVVIKSIGKYQPDAVFIDNVKDLVNDFNNIEESTNVVTTL
                     MQLASKNNCAICNVIHQNVGSAKARGHLGSLLYEKASLALTLKVVAEVTEVNYSKIRN
                     VPPAPFAFSINPETILPELTEIVEPTTATNKLAGIFEGLIENGKTISHTELTHRVMKK
                     CNIKESMAMRKISTALKDGFVFKNNVGMYFSKQPETEVQEINLDIEVRRDTEDEMPF"
     gene            3038..3415
                     /locus_tag="PKH58_12585"
     CDS             3038..3415
                     /locus_tag="PKH58_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89909.1"
                     /translation="MTKKNIQIEKELSLEVEHEKQVELCTKFLDLFFEPYHKWTISSS
                     YHYKHLVERFFGLGTHISNEAFIISAYKFDSKPADNRGLNFYYKFRLKELSSSDVEKI
                     RKINPDVKLNKKIKNGYYTCYSL"
     gene            3453..3620
                     /locus_tag="PKH58_12590"
     CDS             3453..3620
                     /locus_tag="PKH58_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89910.1"
                     /translation="MEKKKKKNELDNSNTDWWENFIPDWEVDPLKDWKVDPFKDWWEN
                     FVPDWGVDDKK"
BASE COUNT         1438 a          624 c          750 g         1019 t
ORIGIN      
        1 acctttttat gaaaagtatg tgtttaccga caaaccgcaa caatccgact ttgacagcaa
       61 cgataacaca aacccactga tttttgacat gcatttgata accggattgc acaataattt
      121 cagatcgttt atttttgaaa cacccgacat tgatacgttt aagaattact tcagggaaag
      181 accgatgccg attaaaaaaa gaaaaggaat aacaatttca gaactttgtt atttgtttgg
      241 aaaaattgaa agcaaacaaa caggcgtttc aaatttcgct gaatggatga aaaaccatat
      301 cgagggcaat aattacggga gcaagaaaaa cgaacttatt gatttggcta aaaatgcaaa
      361 agcgaaaaga aacggttttt cactgacagc accgcaacaa aaagcacttg aaaataaaga
      421 taaaatcgat aaattattta atcggatgca ccccgattga tagttgaaac ctaaaaaata
      481 cctgcaatta tttgttaaat tattcacccc gataattaac cgcttgtaaa catgaaagtt
      541 gcaggcggtt tttttatgta aataagtata agttttttag aaaaatacct tttacatacc
      601 ttttgatgtg tgtgaaattg ccggtacaaa aaggaattaa cctgattgca ttaaatttca
      661 aagttatgga aaaaagaatt tttgaactga ccgtttctga atttatcgaa ttattgaaac
      721 aggaacagcc ggtgcaagtg atgccggagc aaaagaccga accgaaaatg ctttatagca
      781 ttaaagaact tgctaaattt ctgcagtgct caaccgttac ggcacaaaag attaagaaca
      841 gcggaaaaat acctttttcg caaacaggca gaaaagttgt gtttgatggc aatgccgtaa
      901 tgagtgcatt atctgtaaag atgaaagggc aaagagcatg aaaaaagccg aagttaaacc
      961 cccgaccttt aacaatgaca gcaacaaagg taaggtaaaa gaaattactt tcaaaatatc
     1021 cttatggtta tttgttctga ttttcatgta cttattaatt ttcaaatgat atgaaagcgg
     1081 acaaaataac caacgaaaca caggtatttt tcgactacct gcaagagaat gtttgcaccg
     1141 caacgatggt aagcaaagaa acgggaatag agcaaaaaaa tataacaagg tacaaacgag
     1201 gtttagaaaa aatgtacctg ctttatgtga ttgagaaaaa acgatgcccg atcacaggat
     1261 ttccggcaca atggattaca accaatcctt ttatgtttcc gcaaaataat aaacaattaa
     1321 ccttattttg atatggaaac acgacaatat aagaaatgga ataaaaaccc tgaagtaact
     1381 caattttcaa tcgatggcaa acagccacca agcgactcgg atgctgaaga ttatttatta
     1441 ggttgcttaa tgagtgaaag cggtgcaatc gatttagtcg gttcaatcac ttcagatgtg
     1501 ttttatagtg aaccaaacaa agcgattttc agagcttgca agcgactaca aagcaaagac
     1561 agtgtaatta gtccgatatc cgtcaatcgg gaactgacag cggatgagag ggcaattttc
     1621 accgaagcac ccgaattata tctgtatgca attacaggaa aaatcggtac tacaaccgac
     1681 ctcgaaaata cagcttttca cctgaaggat gtttccataa aacgggaaat ggtattgtca
     1741 gcgttacaga ctattacaga tattgacacg ctcgacccgc tcgataatct gacaatcgga
     1801 aaagaccggc tcgtttcgat tgaaaattcg ttttattctg actattcatt tgataaatac
     1861 attgttcccc gacatttgga cttcgaccga cccgaaccaa ttctgtatag aaatgaagat
     1921 gagattttgc accggaagga tttacaattt atcgaagggc aatcagggag caaaaagacg
     1981 tttttagtta gtgcaatgat agccggattg ataggggatg acctcgaaaa atcgatgcac
     2041 ttcaacacta tcaaaaacga agtgaaagtg cttttcattg atacggagca atcaatcggg
     2101 aacgttcaac gtgtggcacg tagaattcac cgtatggcag gaatagacga acgatcagac
     2161 tttgataatt taattgtgtt gggattgaga gaatgtacac ccgttgaacg tttggcagta
     2221 gttataaaaa gtattggtaa atatcaaccc gatgccgttt ttatcgacaa tgtgaaagac
     2281 cttgtaaacg atttcaataa cattgaagag agtactaacg tagtaacaac gttgatgcaa
     2341 ttagcaagca agaataattg tgctatctgt aatgttattc accaaaacgt cgggagcgct
     2401 aaagcaagag gacatttagg cagtttactg tatgagaaag caagtttagc tttaacgctc
     2461 aaagtggttg ctgaagtaac agaggtaaat tacagcaaga tccgcaacgt tccaccggca
     2521 ccatttgcat ttagtataaa tcctgaaacg atattgcccg aactgacaga gatagtcgaa
     2581 ccgaccaccg caacaaataa acttgcaggt atattcgagg gattaatcga aaacggaaag
     2641 acaatttcac atactgaatt aacccacaga gtaatgaaaa aatgtaatat caaagaaagt
     2701 atggcaatga gaaaaatatc tacagcactg aaagacggat ttgtatttaa aaataatgta
     2761 ggtatgtatt tttcgaaaca gccggaaacc gaagtacagg aaattaattt agatattgaa
     2821 gtcagacggg acaccgaaga tgaaatgcct ttttaattaa atttgatttt tatgaaaatg
     2881 tttaaatggt taaggtattt agttagtgta ctggtgtagt gtatttcccc ctctataagg
     2941 aggggggaaa gtacaccgac accgattaac ctaaaataca ctgaaaagta cactaaaaag
     3001 tacactaaaa agtacaccaa taagaaataa taaaattatg actaaaaaaa atattcaaat
     3061 tgaaaaggag ttatcactcg aagttgaaca cgaaaaacag gtagaacttt gcacgaagtt
     3121 tttagattta ttttttgagc catatcacaa atggacaatc agctcatcat atcactataa
     3181 acatttagtg gaacgttttt tcggactcgg cactcatatt tcaaacgaag cgtttataat
     3241 ttctgcgtat aaatttgaca gcaagcccgc agataataga ggattgaact tttattataa
     3301 atttcgattg aaagaattat cgagttctga tgtcgaaaag attagaaaaa taaatccgga
     3361 tgttaagttg aataaaaaaa tcaagaatgg atattacact tgttattcac tctaaaatcg
     3421 gacaaattgg acacgcttaa aaggaaaatg agatggaaaa gaaaaagaaa aaaaatgaat
     3481 tagataattc caacactgat tggtgggaaa attttattcc tgattgggaa gttgatccgt
     3541 tgaaagattg gaaagttgat ccgttcaaag attggtggga aaattttgtt cctgattggg
     3601 gagtggatga caaaaaatga ttagagaata attagataaa agcaacgaat taacaacgaa
     3661 tttgaagtga aagaacagcg aaagaacagt gaaaattccc tcatgtttga acttacatga
     3721 aacgaagcat tttaagccgt tctaacagca cgaaccacga aagcggggaa atgtatcact
     3781 attgaaagat aattcaacag cgttgcggaa aatgagccgt tcaatgtgta t
//
LOCUS       DAOPYB010000254         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_31918_length_3799_cov_11.590278, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000254 DAOPYB010000000
VERSION     DAOPYB010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31918_length_3799_cov_11.590278"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PKH58_12595"
     CDS             complement(<1..893)
                     /locus_tag="PKH58_12595"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX89911.1"
                     /translation="MKKILALLLVLLMFSACKKLEDLNENTKDPTEIPGEALFTGAQL
                     NLFDQMVSSNVNRNIFRMFAQYWTETTYTNESNYDLTSRTIPDNHWDVLYRDVLKDFK
                     ESAKIIGETNYGADPSPKIKSNKLAIIEIMSVYTWSVLVETFGNIPYTEALDIDNLSP
                     KYDDGLTIYKDLISRLDAALVTLDPAWGSFDAADNIYAGDVGLWIKFGNSLKLKMGLI
                     LADKDPTYAQSVVSAAVPNVFSSNLDNARLIYSSSQPNTNPLYEDLVASGRHDFVPAN
                     TIVDVMNTLADPRRPLFFTLAP"
     gene            complement(905..>3799)
                     /locus_tag="PKH58_12600"
     CDS             complement(905..>3799)
                     /locus_tag="PKH58_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX89912.1"
                     /translation="MGGSTNVLLRGNKSLTGDNQVLFVVDGVPINNNNFNTSLQSQGG
                     IGYDFGNMASDINPDDIESINVLKGAAATALYGSRAAGGVVMIVTKKGSAGGNIEVKK
                     KGIGITINSGITAGFVDKSTFPKYQKDYGAGYGHYYAGDPGDEYWYFRYINPDGTITK
                     VPNDPNSVLSQFAVTSEDASYGAPFDENRLVYQWDAIDPESPNFLTATPWIAAKNGPI
                     TFFEKPLTFTNSVSIDNSSSKGSYRLSYTNFTQKGLLPNSKLNKDNVLLNGSWNVTDK
                     LVVSGSGNYTSTRGLGRNSTGYSDNIMTSFRQWMQTNVDIQEQKDLYDLTGRNITWNY
                     ASPSYPVPAYWDNYYWTRYKNYESDNRNRFTGYMAVDYKITKWLDIFGRFSADTYNEL
                     QEERRAVGSIATPFGIGTGADGSLGLSDQESGYLRRDIAFSEYNFDLMGNVNKDFGKN
                     WNLKGVLGLNIRRTNFSRILSATNGGLIVPDLYSLQNSAGPLPLSKELASKVGVNGIY
                     ASVSVGYKSLIYLDGTLRRDHSSTLPVGNSVYYYPSVATSFVFSNLIPENKWLSYGKV
                     RLNYAMVGNSAGFDQLVDNYNFITPLNSPILSVPGTKKNTDLLPEKTRSIEGGLEMLF
                     FGRRLGFDIAYYNSNSSNQILPLAVSTSTGYTYKIINAGQIQNQGVELMVNATPVKSK
                     KFQWNITLNWAKNWNKVLSLAEGVENLQLGSFQGGITINARVGEPYGVICGTDYVYKD
                     GKPIVDPVSGRYIKTNESDKVIGNVNPDWIGGILNSFSYGNWNLSFLIDVSMGGDIFS
                     LDMYYGLATGLYEETSFINDKGNPVRNTLDEGGGVVNKGVNPDGAENTTYMDCSDYGS
                     FGYETFGNMTYDGTSYYLGNPDKAFVYDATFVKLREVSLSYSLPSKWLQKCFITGLTL
                     SAVASNPWIIYKKLPYADPESGLGAGNLQGYSVGSLPSTRDFSFNVKLTF"
BASE COUNT         1191 a          773 c          661 g         1174 t
ORIGIN      
        1 ggtgctaatg tgaagaacaa gggtcttctg ggatcagcaa gtgtattcat aacatccact
       61 attgtattgg caggaacaaa atcatgtctt ccgctggcaa caagatcttc ataaagtgga
      121 ttggtgtttg gctgcgaaga tgaatatatc agtcgtgcat tatcaagatt tgaagaaaat
      181 acattgggta cagcagcact aacaacagat tgagcataag ttgggtcctt atcggcaaga
      241 attaacccca tttttagttt taaagaattg ccaaacttta tccacaggcc tacatcgccg
      301 gcataaatat tgtcagcggc atcaaaactg ccccaagcag gatctaacgt tactaaagcg
      361 gcatctaaac gagatatcag gtctttatat atcgttagtc catcatcata ttttggagaa
      421 aggttatcga tatccaaagc ttctgtataa ggaatatttc caaatgtttc aacaagtacg
      481 ctccaggtat aaacagacat aatttcaata attgcaagtt tatttgattt tattttcggg
      541 cttggatctg caccataatt tgtttctcca ataattttgg cagattcttt aaaatctttt
      601 aaaacatcac ggtataagac atcccaatgg ttatcaggta ttgtgcggga tgtaagatcg
      661 taattgcttt cgtttgtata tgttgtttcc gtccaatact gtgcgaacat cctgaaaata
      721 tttcgattca cattagaact taccatttga tcgaaaagat ttaattgggc tccggtaaat
      781 aaggcctctc cgggtatttc agttggatct tttgtgtttt cattcaggtc ttccaacttt
      841 ttacaagcag aaaacataag aagaacgagt aatagtgcta atattttttt catggctgta
      901 tgttttaaaa ggttaattta acattaaagc tgaaatctcg agttgatggc agtgagccaa
      961 cagaataccc ctgaaggttt cctgcaccta agcccgattc cgggtcggca tacggaagtt
     1021 ttttataaat tatccaggga tttgatgcta cggccgataa agttaatccc gtgataaaac
     1081 atttttgcag ccattttgaa ggcaatgaat atgataatga cacttccctt aatttgacaa
     1141 aagtagcgtc ataaacgaaa gctttatctg gatttcctaa atagtaagat gtgccgtcgt
     1201 aggtcatatt tccaaatgtt tcatatccga aagagccgta atcactacaa tccatatatg
     1261 tggtattttc tgctccgtct ggattaacgc ccttattgac aacaccacca ccttcatcca
     1321 atgtgtttct tacaggattt cctttatcgt ttataaatga agtttcttca tacaaacctg
     1381 tagctaaacc ataatacata tctagcgaga aaatatcgcc acccattgat acatcaataa
     1441 gaaaacttaa attccagtta ccataagaaa aagaatttaa aataccgcca atccagtcgg
     1501 gattaacatt tccaataacc ttatcggatt catttgtttt aatatatctg cccgaaaccg
     1561 gatctacaat tggttttccg tctttgtaaa cataatctgt tccgcaaatt acaccataag
     1621 gttctccaac ccttgcatta attgtaatac ctccctgaaa tgatccaagt tggaggtttt
     1681 caacaccttc ggctaaggat aacaccttat tccagttttt agcccaattt aatgtgatat
     1741 tccattggaa ctttttactt tttacaggcg tggcattcac cataagttca actccttgat
     1801 tttgtatttg tcctgcgttg ataattttat aagtgtagcc cgttgatgtc gaaacagcta
     1861 atggtaatat ctggtttgat gaattactgt tgtaatatgc aatgtcaaaa cctaaacgtc
     1921 ttccgaaaaa caacatttca aggccgccct caatgcttct tgttttttca ggtaataaat
     1981 cagtattttt cttagtcccc ggaactgaaa gtattggcga attcaaagga gtaataaaat
     2041 tataattgtc aactaattga tcaaagcctg cactattgcc aaccatggca taattcaaac
     2101 gaaccttacc ataagacagc catttgtttt cagggatcaa gttagagaat acaaaacttg
     2161 tagctacgga aggataataa tagacactat ttccaactgg tagcgtggaa gaatgatcgc
     2221 gccttaaggt accatcaagg taaatcaggc tcttataacc aacagaaacg cttgcataaa
     2281 taccattaac accaaccttg gatgcaagtt cttttgaaag tggtaatgga ccggcactgt
     2341 tctgaagtga atataaatct ggaacaataa gccctccgtt tgttgctgaa agaatcctgc
     2401 tgaaattggt tcttcgtata tttaagccta atacaccttt tagattccaa tttttcccaa
     2461 aatctttatt aacattcccc ataaggtcaa aattatactc actgaaggca atatctcttc
     2521 tcagatatcc tgattcctgg tcgctaagac ctaaactgcc gtcagccccg gtaccaattc
     2581 caaagggggt agcaatactc ccaacagccc ttctttcttc ttgcaattca ttgtaagtat
     2641 cagcagaaaa acgaccaaaa atatccaacc attttgttat tttataatca acagccatat
     2701 atcccgtgaa acggtttctg ttatcagact cataattctt ataacgtgtc cagtaataat
     2761 tatcccaata agcaggcacc gggtaagatg ggctggcata attccaggta atatttcttc
     2821 cggtaaggtc ataaagatct ttttgttcct gtatgtctac attggtttgc atccattgac
     2881 ggaaagaggt catgatatta tcactataac cggttgaatt tctccccagt ccccttgtag
     2941 aagtataatt acccgagccg gaaacaacca acttgtcggt aacattccac gaaccattca
     3001 gtaatacatt atctttattc agcttgctgt ttggcaaaag tcctttttga gtaaaattgg
     3061 tgtaggataa gcgataagag ccctttgagc tggaattatc aatagaaaca gaattagtaa
     3121 aagtaagcgg tttttcaaaa aatgtaatag ggccattttt agcagcaatc cacggagtgg
     3181 cagtcaaaaa atttggtgat tccgggtcaa tggcatccca ttggtaaaca aggcggtttt
     3241 catcaaacgg agctccgtaa gaggcatctt cagaagttac cgcaaattgc gacaaaacac
     3301 tattcggatc atttggaact ttcgtaatag taccatcggg attaatatac cgaaagtacc
     3361 aatattcatc accgggatca ccggcataat aatgtccgta accggcacca taatcttttt
     3421 gatatttagg gaaagtgctt ttgtcaacaa acccggctgt tataccagaa ttaattgtta
     3481 taccaatacc ttttttctta acttcaatat ttccaccagc actacctttt tttgttacaa
     3541 tcataaccac accaccagca gcacgtgaac cataaagagc tgtagcggca gcgcctttaa
     3601 gcacattgat agactcgata tcatcaggat ttatatctga cgccatattt ccaaagtcat
     3661 atccaatacc gccctgtgat tgtaatgatg tgttaaaatt attattatta ataggtacgc
     3721 catcaacaac aaaaagcacc tgattatctc ctgtaagtga tttattacca cgcaaaagca
     3781 cattggtgga gccacccat
//
LOCUS       DAOPYB010000255         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_31931_length_3798_cov_8.769436, whole genome shotgun sequence.
ACCESSION   DAOPYB010000255 DAOPYB010000000
VERSION     DAOPYB010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31931_length_3798_cov_8.769436"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="PKH58_12605"
     CDS             complement(<1..1738)
                     /locus_tag="PKH58_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89913.1"
                     /translation="MKKILSLFTIFFIFNNLIYTQDQGTLVDFSSIPSAGTIMIFGHQ
                     DDDAIFMLPWWGKTQKFICGAMPSTPTFKATIDELQDSLNINGYGINYKGNWFHPWAD
                     VTQQEYVNYYWLRDTINYGYMANDHLVHELYSYTRPEINKIKAKIEKYIADSTTKRII
                     SNNNWGEYGHTQHKAINQAVRELAVKYRKDTWVLGVNGNLDDLVVPVDVTYTIANNDT
                     NLYKTIRNTYANHGVWTYSTTSLPDRPYKYIKVVDDGVDKSNLAISDTFSISTSGPPQ
                     DKPGSYILNGTSDYLTLPGNNYSSFTIAMWVRPDEIRAMDISKMSEYPSYSTCNRSFY
                     MLADGKICARVSDGIATSRTTLSAGVWSHILMKYEGNNLTLYINGVPEDTIATSALTQ
                     YSSPEFILGQAQETASFLKGQILDVRLYDYALSDNEVQLVSSSNSPTTYLITSTTGTG
                     GTINPLGDVILNEGGSRTYTITPGTNFQISDVKVDGESIGAVNTHTFSNIKANHTISS
                     SFSQNLLTGIALNKPATAQSYTGYNTPGKANDEDGSSASYWGASSYPQWWQVDLQDFY
                     DISSVYIRNYYDG"
     gene            2340..3164
                     /locus_tag="PKH58_12610"
     CDS             2340..3164
                     /locus_tag="PKH58_12610"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX89914.1"
                     /translation="MKLEFLLLGLISIFSFSNISGQKIVTGKIINRETNEPVEYVNIG
                     IQGKETGTVSDNQGNFQLLIADKNLNDTLTVSCIGFNIAKVGISKLINADHKNILLTE
                     KAIDLKEILVKPKKYKTRILGITAKNKKILGGFTENKLGYELGVAMSAKKSAYIRKIN
                     LNIASCEYDTVFYRLNVYEGTLKHGFENIMREPFYFKIPKSQIKDKISIDVQKLNLVV
                     RGNFLVTLEYVKDLGKGGLYFPISVFNKSYARKTSQGEWKTTPVGISLNIEADVEQ"
     gene            complement(3382..>3798)
                     /locus_tag="PKH58_12615"
     CDS             complement(3382..>3798)
                     /locus_tag="PKH58_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89915.1"
                     /translation="FAISGVRLFFYFTRLTIKLVIALVITIPLGVKAMVIGHVVTSAI
                     AFFINAYMPGKLFGYGAFAQLKDMVPVFIASAAMALFVYIFEYFIDNLYLKLIVGGIV
                     GLSTYLIMSYLLKIEELKEFTELIIKTKKRFGKASA"
BASE COUNT         1256 a          615 c          715 g         1212 t
ORIGIN      
        1 ccccgtcgta gtagtttctg atgtacaccg aactgatatc gtaaaagtcc tgcaggtcta
       61 cctgccacca ctgcgggtaa gatgatgctc cccagtagct ggcacttgaa ccgtcctcgt
      121 cattggcttt acccggagta ttatagcccg tatatgactg tgcagtagca ggtttgttta
      181 aggcgatacc agttaaaaga ttttgactga aagaagatga aatagtgtga tttgccttga
      241 tattcgaaaa cgtatgagta ttaactgcgc ctatcgattc tccatccact ttgacatcgg
      301 aaatttggaa attggtaccg ggagttattg tatatgtgcg cgatcctcct tcattaagta
      361 taacatctcc aagtggatta atagtacctc cggtacctgt ggtagatgtt atcaaatatg
      421 ttgtagggga gttagaactg cttacaagtt gaacttcatt atcagataaa gcataatcat
      481 acaatcgaac atccagaatt tgccctttga gaaatgaggc agtttcttgt gcttgtccga
      541 gtataaattc aggtgatgaa tattgtgtta atgcacttgt cgcaatggtg tcttcaggca
      601 ctccgttaat ataaagagtc aaattattac cctcatattt cataagtatg tgagaccata
      661 cacctgcgga taatgtagtt ctggaagttg caatgccatc tgaaacccgg gcgcaaattt
      721 taccgtcagc cagcatataa aaactgcgat tacaagttga atatgaaggg tactcagaca
      781 tcttcgaaat atccatggcc cggatttcat caggtcttac ccacatggca atggtaaatg
      841 atgagtagtt attaccgggc aaggttaaat aatcagatgt accattaaga atgtatgagc
      901 ctggtttatc ttgtggaggt cctgaagttg aaatgctaaa tgtatcagat attgcaagat
      961 tagatttatc aactccgtca tctacaactt taatatattt atagggtctg tcaggtaaac
     1021 ttgtggtact gtaggtccat acaccgtgat ttgcataagt gtttctgata gttttataaa
     1081 ggttagtgtc attgtttgct atcgtatagg taacatcaac tggaacaact aaatcatcca
     1141 gattaccgtt tactccaagt acccatgtgt ctttacgata ttttactgcg agttctctta
     1201 ctgcttgatt aatagcttta tgctgtgtat gtccgtattc gccccagttg ttattcgaaa
     1261 taattcgttt tgtggtacta tcggcaatgt atttctcaat cttagctttt attttattta
     1321 tttcaggcct agtatatgaa tagagttcat gaacaaggtg gtcatttgcc atatagccat
     1381 aatttatagt atcgcgtaac cagtaataat tcacatactc ttgctgggta acatctgccc
     1441 agggatgaaa ccagtttccc ttataattta ttccatagcc attgatattg agagaatcct
     1501 ggagttcatc gatggtagct ttgaaggtag gcgttgatgg catagctcca catataaact
     1561 tctgagtttt gccccaccat ggcaacataa atatagcatc atcatcctga tgtccaaaaa
     1621 tcatgattgt tccagccgaa ggaatgctac tgaaatcaac aagtgtgccc tgatcttgtg
     1681 tgtaaatcag gttgttgaat atgaaaaata tagtaaacaa ggatagaatt tttttcataa
     1741 caaaagaatt tagatattaa ttaacccgtt tctgtaaata gaaccatatc tgtattgatt
     1801 ttttgaaata tatactgtcg aaaccgcgag tatatttata gtttataaca aattaatgcg
     1861 tgtgaatatt aaatcttaac aaacaatgct ttaatgataa atttttgttt atacattaaa
     1921 catattatat caacgaaaag tttttgaagt tttatttttg ttgtcggaat atagtggttt
     1981 ttctttcaat attttattaa ttgaaaatta cgagtgaaag aaaatatgag tggttcatgt
     2041 tgagtataag gaagacttta aagcctgtct gctttctgct gacaggaaat ttataacatg
     2101 gtaacagaga agttgtgttg gatttattga ttcgaaattg cccttcaagg catttcttgt
     2161 atgaattcac ctgtattgtg tttgttaaat tatttgcata aatttgtaga taaaaagagg
     2221 gttaggatat aaatttttaa cataggtatg tatttcatga aacaaagcca gaaccgctga
     2281 ggcagaaaag actttaaagc tttaatcatc aattaatcat cttttatata tacatgaaaa
     2341 tgaaactaga atttctcctg ttgggtctga tatcaatttt ttctttctcg aatatcagtg
     2401 gtcaaaagat tgttaccggc aaaataatta atcgggaaac taatgaacct gttgaatacg
     2461 taaatatagg gatacaggga aaagaaaccg gtactgtgag cgataatcag ggaaattttc
     2521 agcttcttat cgccgataaa aacctgaatg acacattgac agtgtcctgt attggtttta
     2581 atattgcgaa agtagggata agtaagttga ttaatgctga tcataaaaat attttattga
     2641 ccgaaaaagc aattgatttg aaggaaatat tggtaaagcc aaagaagtat aaaacaagaa
     2701 ttctcggaat tacggcaaaa aataaaaaga ttctaggagg ttttactgaa aacaaattag
     2761 gttacgaact gggtgtggcg atgagtgcaa aaaaatctgc atatatacga aaaatcaatt
     2821 tgaatatcgc atcctgtgag tacgatactg ttttttatcg tttaaatgtc tatgaaggaa
     2881 ccctgaaaca tggattcgaa aatattatgc gtgaaccatt ttatttcaaa attccaaaat
     2941 cgcaaataaa ggacaaaata tccatcgacg tacaaaaatt aaatctggta gtaaggggaa
     3001 attttctggt gacattggaa tatgttaaag atctggggaa aggtggcctg tattttccaa
     3061 tctcagtttt taataaatca tatgcccgaa agacaagcca gggtgaatgg aaaacaacac
     3121 ccgttggaat ttccctcaat atcgaagcgg atgttgagca ataatttttg catataatgc
     3181 acttcctttc gcgccaattt ataacccgaa tgttacttct caaagtaaaa attcattagt
     3241 ttgccattac aaagtaaact acaaattgtg aggagacttt atctggaaga aagaaatttg
     3301 agggtgtggt tttaataact gatagcgggc agctgaaaaa gaaaagcctt tgcaataatc
     3361 gatttaacga tacaatgatt tttaagcaga ggctttgcca aaacgctttt ttgtttttat
     3421 tataagttct gtaaattctt tcaattcttc tatttttagc aggtaactca tgatcagata
     3481 tgttgataaa ccaacaatgc ctccaactat caactttagg taaagattat ctataaaata
     3541 ctcaaagata tatacaaaca atgccattgc agctgaagcg ataaaaacag gtaccatgtc
     3601 ttttaactga gcgaaagctc cgtaaccgaa aagcttaccc ggcatatagg cattaataaa
     3661 gaaagcaata gctgaagtaa ctacatgtcc gattaccatg gctttaacac ctaaaggaat
     3721 agttataacc aaagctataa ctagtttaat cgttaatcgt gtgaaataaa aaaataatct
     3781 aacaccagag atagcaaa
//
LOCUS       DAOPYB010000256         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_32005_length_3793_cov_7.598181, whole genome shotgun sequence.
ACCESSION   DAOPYB010000256 DAOPYB010000000
VERSION     DAOPYB010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32005_length_3793_cov_7.598181"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /locus_tag="PKH58_12620"
     CDS             complement(<1..1875)
                     /locus_tag="PKH58_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89916.1"
                     /translation="MINNLFVKGITCLLIGFLSMSTQASKASVDPYLVAHFPMEVQGN
                     AIFETVSGKSFVVSNNFNRPENIPGAEGNALRLDGYSTFCNAQINASTLNSTALSVSL
                     WCALETYPVMDPNNEVTTETYIAGNMNENLKTGFAFTLNGVGAFSFQVYVDGNKITCA
                     DFNQTLKAYDWHHLVATVNSTTKEVNLYKNGELISSSYFFGKGVINTGANDFMIGKSF
                     ANTYADVFRTNTINGLVDDIRIFSRVLSGSELTNKVPEHVADLSIPVTRHQDDIQRPE
                     FHGLPATNWTNEPHGMVYFNNKYHIFFQKNANGPYWGKLHWGHISSDDMLNWKEEKIA
                     LANTSGYDIKGVWSGCVFTDDVLTGGKPNIFYTGADYAKTSINQAEPVDDNLISWTKN
                     SKNPIIPNRPGGLSDDFRDPYVFKSNGNYYMIVGTAKNGLGAATLHRYDPATKTWSND
                     GSIFYQSSSIDYGFFWEMPVIVPMPDGKWLFLVTTLGGKQGVENLYWVGTINSDGTFN
                     PYSDTPKEVELGTFGTNGYGLLSPSVMQKDGKTIAVGIVPDKLSSADNLRLGWAHLYS
                     LPREWSLDANNNLIQIPFSGIQNIRSAETVYNIVNEEVNSTCSLSPVSGKTLEIDGSF
                     I"
     assembly_gap    2176..2275
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2276..2972)
                     /locus_tag="PKH58_12625"
     CDS             complement(<2276..2972)
                     /locus_tag="PKH58_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNX89917.1"
                     /translation="MKKYIKIYFVLLILPFLIASCLNKEEFNVNPNLQSVSNLQSALV
                     GDSVKLTWTLPASTTPLKVMISSDTQTFVQLPANSTSVTWGIVEVNKPYVLTIKVADD
                     NGNTSLGQSVSFTREGTNPVENLDAKRNPTDIVVTWTLPQQNTATSIELTYDNQVVNL
                     PITATSYRILNVISDKKYVIKVRTKSATLTSHYVTKVVNSLNFAFLTTFETLTALQAN
                     GDDDEIAAANWFVA"
     gene            complement(2997..>3793)
                     /locus_tag="PKH58_12630"
     CDS             complement(2997..>3793)
                     /locus_tag="PKH58_12630"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX89918.1"
                     /translation="GDGFFQPSQNLVNSYKTDANGIPQIDTYNTSNLMTVADGLAVNV
                     DPRLDFTVGRIGVRWKDYSASPYDINWSREPATYGTYGTKKFLVSPNSPNLVKGWPWG
                     GSSLNYQVIRFADVLLWKAEALIELGRQSEALPLINAVRMRAKNSKYVLAWNNTSSTD
                     FAAKYKISAYQDGVNCSWTQDFARKALRFERKLELAMEGERFFDLVRWGVADQVLNTE
                     YFAKEKEIRDYLKNAKFVKGRDEYFPIPQNQINYSAGLYSQNPGYN"
BASE COUNT         1057 a          782 c          701 g         1153 t
ORIGIN      
        1 gatgaaactt ccatctattt cgagcgtttt acccgaaaca ggcgataatg aacatgtaga
       61 gtttacttcc tcattaacta tattataaac cgtttccgct gaccggatat tttgaatacc
      121 cgaaaaaggt atctgtatga gattattgtt tgcatccaag ctccactccc tgggcaggct
      181 gtataaatgt gcccacccta accgcagatt gtcagctgac gaaagtttat ctggaacaat
      241 acccacagca atggtttttc catctttttg catcacagaa ggcgaaagta gcccataccc
      301 gttagttcca aaggttccaa gttccacttc ttttggagta tctgaatagg gattaaatgt
      361 tccatcggag ttaattgtac ctacccagta caaattttcc acgccttgtt ttccacccag
      421 tgtggtaacc agaaacaacc atttgccatc aggcattggt acaataacag gcatttccca
      481 gaaaaaaccg taatcgatgc ttgatgactg gtagaaaata gaaccatcgt tgctccatgt
      541 ttttgtggcc ggatcatatc tgtgcaacgt tgcagctcct aatccatttt ttgcggttcc
      601 aactatcatg tagtaattac cgttcgattt gaaaacatac ggatcacgaa aatcatcact
      661 taaacctccc ggacgatttg gaattatcgg atttttactg ttttttgtcc agctgatcag
      721 gttatcatca acgggctcag cctgattaat actggttttg gcataatctg ctccggtata
      781 aaatatattc ggttttcctc ctgtcaatac atcgtcggta aaaacgcaac ccgaccaaac
      841 tcccttgatg tcgtaaccac ttgtattggc taatgcgatt ttctcttctt tccagttcag
      901 catatcatca ctgctgatat gcccccagtg cagttttccc cagtaaggtc cattggcatt
      961 tttctgaaag aagatatgat atttgttgtt gaaatatacc atcccgtgtg gttcatttgt
     1021 ccagttggtg gcaggtaaac catgaaattc aggtcgttga atatcatcct gatgtcgtgt
     1081 taccggaata gataaatcgg caacatgctc cggaactttg ttggtaagct ctgagccgga
     1141 taacactctg gaaaatatgc ggatatcatc cactaaacca ttaatcgtat ttgttcggaa
     1201 aacatcggcg taggtgttcg caaatgattt tcctatcata aagtcattgg ctccggtatt
     1261 aattacaccc tttccaaaga aataggaaga gcttattaat tcaccgtttt tgtacagatt
     1321 tacttctttg gtagtgctat tcaccgtagc aaccaaatga tgccaatcgt aggctttcaa
     1381 cgtctgatta aagtcagcac aagtaatttt attcccatca acatacacct gaaaactaaa
     1441 tgcacccaca ccgtttaatg taaaggcaaa acctgtttta aggttttcgt tcatatttcc
     1501 ggcaatgtaa gtttcggtgg taacctcatt gttcgggtcc atcaccggat aagtttctaa
     1561 agcacaccaa agcgagaccg atagggcagt agagtttaat gtcgaagcat ttatttgagc
     1621 attgcaaaag gttgaatatc catccagtcg cagtgcgtta ccttcagccc ccggtatgtt
     1681 ctctggtcga ttgaagttat tgcttacaac aaagcttttg cccgataccg tttcgaaaat
     1741 agcatttccc tgtacttcca tcgggaaatg agccactaaa taaggatcaa cagaagcctt
     1801 ggaggcttgt gtggacatcg acaaaaaacc tattaacaaa caggttattc cttttacaaa
     1861 cagattatta atcatgatat actgcgtttt tttagtttgt atttaaagtc aatatacaac
     1921 gaattactga tagccttatt acaaaaattc tttttaataa tggaactggt taatatgcac
     1981 tgaaccgttt gattgaaaaa acaagaaaca atctcctttt aataagaaga ttgtttcttt
     2041 atacacttac tatttgagta aatcaaatgc gtttttagta attttttcga tattagcctg
     2101 atgcaaattc gtatttgaat tctgacgcca ttcgtacgca gccagtccta tagcataaca
     2161 tttgccctga tattcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntagct
     2281 acaaaccagt tagcggcagc aatttcatca tcatcaccat tagcctgcaa ggcagtcagc
     2341 gtctcaaaag tggtgaggaa tgcaaaattc aaactattta ccaccttcgt aacataatgg
     2401 gaggttaatg tagccgattt tgttctcact ttaataacat attttttatc cgaaatgaca
     2461 ttcagaatac gataagaagt agcagtaata ggcagattaa caacctgatt gtcatatgtc
     2521 agttcaatac ttgttgctgt gttttgttgc ggcaacgtcc atgttaccac aatgtcggtt
     2581 ggattacgct tagcgtccag attttcaaca ggattggtac cttcgcgtgt gaatgaaacg
     2641 gattgtccaa gtgaagtgtt gccgttgtca tcagctacct tgatagtaag cacgtatggt
     2701 ttattaactt ccacaatgcc ccaggtaacg gaagttgagt tagcaggtaa ctgaacaaat
     2761 gtttgagtat cagaactgat catcactttc aaaggagtag tgcttgcagg taatgtccat
     2821 gtaagtttca cactgtcgcc cactaaagca gattgcagat tactgaccga ttgcaggttc
     2881 ggatttacat tgaattcctc cttattcagg cagcttgcaa tgagaaacgg cagaataagc
     2941 agtacgaagt aaattttaat atattttttc atcgtcgtat tttttattgt ggttatttag
     3001 ttatatcccg ggttttgcga gtacaatccg gctgaataat taatctggtt ttgaggaatg
     3061 gggaaatact catcacgtcc ttttacgaat ttggcatttt tcagatagtc ccggatttct
     3121 ttttctttgg cgaaatactc agtattcaac acctgatctg ccactcccca gcgtaccagg
     3181 tcaaagaaac gctcaccttc catggctaat tccagtttac gttcaaaacg caacgctttg
     3241 cgggcaaaat cctgtgtcca cgagcagttt acaccatcct gataagcact gatcttgtat
     3301 ttagctgcaa agtcggttga acttgtattg ttccaggcta atacatattt gctgtttttt
     3361 gcacgcattc ttacggcatt aatgagcggt aaagcttcac tctgacgacc aagttctatt
     3421 aaagcttctg ctttccacaa cagtacatcg gcaaagcgta ttacctgata gttgagtgac
     3481 gaaccacccc atggccatcc ttttacaaga ttgggtgaat tgggtgaaac cagaaatttc
     3541 ttcgtaccat atgtgccata agttgccggt tcacgggacc agttaatatc ataaggtgaa
     3601 gctgaataat ctttccagcg aactccgata cgtcctaccg taaaatcgag acgtgggtca
     3661 acgttaactg ctaagccatc agctacagtc attaaattac ttgtattgta agtgtcaatc
     3721 tgaggtattc catttgcatc tgttttgtat gagttcacca aattctgact tggctgaaaa
     3781 aaaccatcgc cac
//
LOCUS       DAOPYB010000257         3750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_32535_length_3750_cov_10.816509, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000257 DAOPYB010000000
VERSION     DAOPYB010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32535_length_3750_cov_10.816509"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PKH58_12635"
     CDS             complement(<1..599)
                     /locus_tag="PKH58_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNX89919.1"
                     /translation="MSKLTTNSPLRACPGLVTGGLRSLGHGFVRVAAAIPELRVSDCA
                     FNAAKIKELIVKAEAENAEIVCFPELCITAYTCADLFFQKQLLDNAENALADLVADTA
                     GLEITSIVGMPVRKNNQLFNAAVVFKQGEILGVIPKINLPNNNEFYEKRWFTSGYFAT
                     AENIELAGQEVYFGTNYYFRTNNFTFGIEICEDLWMPIPP"
     gene            complement(726..1118)
                     /locus_tag="PKH58_12640"
     CDS             complement(726..1118)
                     /locus_tag="PKH58_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNX89920.1"
                     /translation="MEELTLITPTFLFSAVSLILLAYTNRFLSYAQLVRTLKDRYMED
                     RSAITKAQIVNLRKRLRLTKQMQVFGVVSLLLCVATMFLIYIGYQLISVYIFGVALVC
                     LIISLGLSVREILISSNSLEIYLSDMEK"
     gene            complement(1206..2423)
                     /locus_tag="PKH58_12645"
     CDS             complement(1206..2423)
                     /locus_tag="PKH58_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNX89921.1"
                     /translation="MYLNTEIAKDIYYVGVNDRIKHKFENMLPLPYGVSYNSYLITDE
                     KIALVDTVDVCYSEIFIEKIQSQLKGKQIDYLIINHMEPDHSGSISFIRKYYPNITVV
                     GNKKTLEMLNGYYCIDDNTLEITDGETLSLGRHQLQFHLTPMVHWPETMMTFDLTENV
                     LFSGDAFGCFGTLDGGVIDTQMNTDKYWDEMYRYYANIVGKYGSPVQKALQKLTPLDI
                     NVICSTHGPVWKEQVSKAVDIYDQLSRYEAREGAVVVYGSMYGNTEQMAEAVAQGLVT
                     GGVRNVVMHNISKSDASDILRDIFKYKGLIIGSPTYCNELHPEIDSLLKKIEIRAIKK
                     REFAYFGSFSWAGVANKKLAEFGEKMKWRTASFTVEEKMGLKENNYLSCIQLGKKMAE
                     LLKTPETSEPACH"
     gene            complement(2640..3002)
                     /locus_tag="PKH58_12650"
     CDS             complement(2640..3002)
                     /locus_tag="PKH58_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNX89922.1"
                     /translation="MNEVDCYIAGFPPEVQQILHEIRGIIIGAAPDATEGMAYGIPAY
                     KTNGKPLVYFAGFKKHIGFYATPTGHSAFAAELAHYKQGKGSVQFPLNKPIPYDLIRR
                     ITVFRALENNAKQRAKRG"
     gene            complement(2999..3265)
                     /locus_tag="PKH58_12655"
     CDS             complement(2999..3265)
                     /locus_tag="PKH58_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNX89923.1"
                     /translation="MKALTEMPNVGKVLAQKLIEVDINTPNELISTGSENAFIRIRTV
                     DPGACIDMLYALEGAIQGIRWHSLSPGRKEELTAFFRQTRTQKP"
BASE COUNT         1053 a          751 c          767 g         1179 t
ORIGIN      
        1 ggcggtatcg gcatccagag gtcttcgcat atttctatcc cgaaggtgaa attattcgtg
       61 cggaaataat agttggttcc gaaataaact tcctgtccag cgagttcaat gttttctgcg
      121 gttgcaaaat aaccggacgt gaaccatctt ttttcataga actcgttgtt gttgggtagg
      181 ttgatttttg gaataactcc gaggatttcg ccctgtttga aaactaccgc cgcattgaaa
      241 agctggttgt ttttccttac cggcattcca acaatactcg taatttccag tccggcagtg
      301 tcagcaacca gatcagccag ggcattttca gcattatcca gcagttgttt ctgaaaaaac
      361 aaatcggcac aggtgtaagc cgtgatgcac aattcgggaa agcaaacaat ctctgcgttt
      421 tcggcttccg cttttactat cagttccttg attttagcag cattgaaagc acaatcggat
      481 acccgaagtt cgggaatggc agccgctact ctgacgaaac cgtggcccag gctccttaac
      541 cctcccgtaa caagtccggg acaggctcta aggggagaat tagtagtaag tttgctcatc
      601 gtttgtggtt tttatttagt ggtattgtat tttttgagaa cttgtgaata ctttatgaat
      661 attgaatttc tgcaccaata ttcccccttt agggggttag ggggcaggta atcgggtccg
      721 gttgtttatt tttccatatc actcaaataa atttcaagcg aattggacga aataagtatt
      781 tcacgtacag acaagcccag tgaaataatc agacatacca gcgctactcc gaaaatgtaa
      841 acagaaatca gttgatatcc gatataaatc agaaacattg tagccacaca caataataaa
      901 ctgacaaccc cgaaaacctg catttgtttg gtaagtctca atcgtttacg caagttgaca
      961 atttgcgcct tagtaatagc ggaacggtct tccatgtagc ggtctttgag tgtacgtacc
     1021 aactgcgcat aggacagaaa ccggtttgtg taagccagca gtatgagcga cacagccgaa
     1081 aatagaaacg taggggtaat cagcgtaagt tcttccataa cagtgtgatt tgaacatgtg
     1141 taaactatca gaattctaat ctaacttatc agaaacaatg aaaaaactgc gactgcattg
     1201 cagacttaat ggcaagccgg ttcagaagtc tcaggtgttt ttagtaattc agccattttc
     1261 ttgccaagct gtatgcagga cagataattg ttttctttca gtcccatttt ttcctctacc
     1321 gtgaaacttg ccgttctcca tttcattttc tcgccgaatt cagccagctt tttgttggca
     1381 actccagccc atgaaaaaga accgaaataa gcgaattctc ttttcttaat ggctctgatt
     1441 tcgattttct tcaacaaact gtcgatttca gggtgaagtt cgttgcagta tgtaggactt
     1501 ccaatgataa gtcccttgta cttgaaaata tctcgaagaa tatcggaagc atcagatttg
     1561 gatatattgt gcatcactac atttctcaca ccaccagtaa ctaatccctg tgccaccgct
     1621 tcggccatct gttcggtgtt tccatacata gagccgtaaa ccacaacagc accttctctg
     1681 gcttcgtagc ggctcagctg gtcgtaaata tcaactgctt tcgatacttg ttccttccaa
     1741 accggaccat gtgtggaaca gataacgttt atatcaagcg gagttaattt ttgaagggct
     1801 ttctgaaccg gagaaccata ttttccaaca atgttggcgt agtaacgata catttcatcc
     1861 cagtatttgt cggtattcat ctgagtatcg attacaccac catcgagcgt gccgaaacat
     1921 ccgaaagcat cacccgaaaa aagcacattt tcggtaaggt cgaaagtcat cattgtttcc
     1981 ggccagtgaa ccataggggt gaggtggaac tgaagctgat gccgtcccaa agaaagtgtt
     2041 tcaccatcgg tgatttccag ggtgttatca tctatacagt aataaccgtt cagcatttcc
     2101 agtgtttttt tgttgccaac aacggtgata ttgggataat actttcgaat gaaactgata
     2161 gaaccggaat ggtcgggttc catgtgattg atgatcaggt aatctatttg tttgcccttg
     2221 agttgcgatt ggattttttc gataaaaatt tcgctgtagc aaacgtcaac agtatcaaca
     2281 agggcaattt tttcatcagt aataagatat gaattgtatg aaacgccgta aggaagagga
     2341 agcatatttt caaatttatg ctttatgcgg tcgttaacac caacgtaata aatatctttt
     2401 gcaatttccg tgttcaaata cataatcttt tttatagttt ttttcagtta acaggttatt
     2461 tgtcagaatc agatatttga ataatcattt ttcaattaat aacatctgat cttaatgatt
     2521 gttttttaca tttgaaatgc aaagatacaa attatttgcg gaaaggataa aaacggagta
     2581 cccggaagcc cgaaaatata acgaaacctc ttcgctgaag ctaactggcc gatttcgcgc
     2641 tatcctcgtt ttgcacgttg ttttgcattg ttttccagtg ctctgaacac cgttattctt
     2701 cgaatcagat cgtatggaat gggcttattc agcggaaact gtacagaacc ttttccctgt
     2761 ttgtaatgtg ccagttcagc agcaaatgca ctgtgaccgg tgggagtggc ataaaatccg
     2821 atgtgttttt tgaatccggc aaaatacacc agcggtttgc cgttggtttt gtaagcgggt
     2881 attccgtaag ccatcccttc ggttgcatcg ggagctgccc cgattattat cccgcggatt
     2941 tcgtgaagta tctgctgtac ttcaggagga aagccagcaa tataacagtc aacctcattc
     3001 atggtttctg agtgcgggtt tgacgaaaga atgcggtaag ttcttctttt cgtccgggtg
     3061 ataaactgtg ccaccgtata ccctggatgg ctccttcgag cgcataaagc atatcgatgc
     3121 atgctccggg atcaactgtt ctgatgcgta taaacgcgtt ttcacttccg gttgagatca
     3181 gttcattggg tgtatttatg tccacttcga taagtttttg cgccagtact ttcccaacgt
     3241 ttggcatttc ggtaagtgct ttcattcttg ttgtttgctg agtctgtttt cctgaaaagt
     3301 caatctgttt cagcagattg actgccttga tgatttgtca aaagtagaaa aaatatttga
     3361 gttgatgttt cttttcattt gtttatttat agtttctgat tttaattagt ccaatatctg
     3421 atagttaata tccggtttaa gtgttaatgt ttacaatctt tttaaatcgt gaaaatatgg
     3481 ctaaattttt ataatatctc agggatatta aatttaaaga aaattaattt cgacaatcaa
     3541 taataatact ttcatccgat gcttaattag aaatcagaca attaagaaac tcctttcagt
     3601 aaagctcact tcttttatgc atgaaagaga tattttttaa ctacttttgc tttgaaaaaa
     3661 agaatagaaa aatgatcgtt tgtatagccg aaaaacccag tgtggggcgt gacattgccg
     3721 agatactcgg agccaaaacc cgtaaggacg
//
LOCUS       DAOPYB010000258         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_33271_length_3694_cov_8.900797, whole genome shotgun sequence.
ACCESSION   DAOPYB010000258 DAOPYB010000000
VERSION     DAOPYB010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33271_length_3694_cov_8.900797"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1654)
                     /locus_tag="PKH58_12660"
     CDS             complement(71..1654)
                     /locus_tag="PKH58_12660"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89924.1"
                     /translation="MKKVLILVIFLISVITKNVFCQTWIRYYGQGLNAVCRSVTNDYD
                     HGYVIGGMVNNYKYIWVLKTDINGNILWDKRIGNGINDGGISNIEKTTDGGYILCGSW
                     TKLNPSWDAFIIKLNPCAEIEWCKVLETPDNFDRGMKVIQTFEGDYLLMGAYFITNPE
                     SNISLFKFNPIGELVWHQFYPYEFFYNQDQPCDLLVDTDGYLVTASRYAPDSGTISPQ
                     IDRSYFIKTDTAGNKLWDLVYGINNHYYSDVWGTVRNSMDKYYHSCTHSGVTSTNPGL
                     IWISHDGSEAGDKDIFETNSYNFCGLPTITLLHDTNLIFFGGFTINGSFQSQVIKTDT
                     LGNILKSSGIPEVVNYNSTTKTFDDKFITVADDDYNSKLRICAIKVNSNLEYDSIYTQ
                     PFTYDSLCPHPIVSDTIDPNCDNVIVSVDEPFKKPETTQLKVYPNPTDKNVTIELPKY
                     LVVNDNSGNIPATTIYHQWSSATLQAIDLQGKTVLQHEVANTGVPLQLDVSLLPAGMY
                     QFRLVYRGKPVAGSKVVVK"
     gene            complement(1704..>3694)
                     /locus_tag="PKH58_12665"
     CDS             complement(1704..>3694)
                     /locus_tag="PKH58_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89925.1"
                     /translation="LADSITGIYSYNSGFGVNSLCHSNVIPCDDTIHSEFNGLKYGIK
                     AICSGVLKYIKIDDCLFSNNLRSVYLSGINVPEITRNTIHTKIATQTIPSCGIYLDNS
                     TGYSLQENTITGNYNGSNTYETGIIINNSGTADNLVYNNILSFLQEGIKAQNINRSSN
                     GITGLVCKCNDFTGNKSDEVVFLSQQNQNWGIALYQGDSLSETGPAGNTFSQRSMIKT
                     YDLQNQGARFKYYHQPNTSNKRLKPETQFCSNVYIKQVIYAYYEPTSCPSKLSNGNGI
                     PIEEANEYLHTINGTIEQKESELYTLVDGGSTEEITENIVNSVPPEALELRDNLLSKS
                     PYLSDTAMQTAVSNEFVLPNVMIRDILVANPQSAGSESVLNELDKRIDPMPEDMYNQI
                     LAGESVYSPLEVKEMELASLKLERAQLFDYLLASKMTDTTVSLIDSLTTFLQNEQTLE
                     AKYLLATQQIYHGDTVDAFNTLESIPVDFELSPQQQTVYTEYNSYAAILKNIVNDTLC
                     GMQADSIQTVALMALVEEAHEPIKSYARNMLLSIGRVKYSEPYLYSDELKSERIKKEH
                     KMNPSQQNDYLKIYPNPANSYIIVVYNLEKLLDEFVSTASIEISSIQGQVIKKIILDR
                     KIDQKVVSTQLLFPGIYVVSLKVRDKTIETKKVNIIHP"
BASE COUNT         1056 a          713 c          697 g         1228 t
ORIGIN      
        1 atggcaggcc agggaacagg tcatttcctt ctccgggaaa attccccgcc cgcgtacgcg
       61 ggaatcttta ttattttacc accactttac tccccgccac cggcttgccc cggtacacca
      121 gccggaactg gtacatgccc gccggcagga gtgaaacatc caattgtaaa ggaacgcctg
      181 tattggcaac ttcatgctgc aacacggttt tgccctgcaa atcaatggcc tgcagcgtgg
      241 ccgacgacca ctggtgataa atggtagtgg ccggtatatt tccgctgttg tcgtttacca
      301 ccaggtattt gggcaattct atggtgacgt ttttatcggt aggattaggg tacaccttta
      361 gctgcgtggt ttccggtttt ttaaaaggct catctacact tacaattaca ttatcgcaat
      421 tggggtctat ggtatcgctg acaatagggt gcgggcacag gctgtcgtag gtaaagggtt
      481 gagtgtagat gctgtcgtat tctaagttgg agttgacttt aatggcacaa atccttagtt
      541 ttgaattgta gtcatcgtca gcaacggtaa taaatttatc gtcaaatgtt tttgtggttg
      601 aattataatt aaccacttcc ggaatgccgc ttgatttaag tatattgcct aatgtatcag
      661 tttttatgac ttgtgattga aaactgccat tgatggtaaa cccaccaaag aagatcaggt
      721 tggtatcatg cagtagcgtg atggttggca gtccgcaaaa attgtaacta ttagtttcaa
      781 aaatatcctt atcacctgct tcactcccgt catggcttat ccagataagg cccggatttg
      841 tactagtgac ccctgagtgg gtacaggaat gatagtactt gtccatactg tttctgactg
      901 taccccaaac atcactataa tagtggttgt tgatgccata cactaaatcc cagagtttat
      961 taccggcggt atctgtcttt atgaaataac ttcggtcgat ttgaggggaa atggtgcctg
     1021 aatccggagc atagcgggaa gcagttacca gatatccatc tgtatcaacc agtaaatcac
     1081 aaggttggtc ctggttgtaa aagaattcgt atgggtaaaa ctgatgccaa actagttcac
     1141 ctataggatt aaacttgaac aatgatatat ttgattcggg gtttgttata aaatatgctc
     1201 ccattaatag atagtctcct tcgaaggttt gtatcacttt cataccccta tcaaaattat
     1261 caggtgtctc caacacttta caccactcta tttcagcgca gggatttaac tttataatga
     1321 atgcatccca ggaaggatta agttttgtcc atgagccgca aagaatatag ccgccatcag
     1381 ttgtcttttc aatattactg atgccgccat catttatgcc attaccaatc cttttatccc
     1441 ataatatatt cccgttaata tcagttttca gtacccagat atatttataa ttattgacca
     1501 ttccaccaat aacatatcca tggtcgtaat cattcgtaac gcttctgcaa acagcattaa
     1561 ggccctgccc ataatatctt atccatgttt gacaaaaaac atttttcgtt ataactgata
     1621 ttaagaagat aaccagtatg agtacctttt tcatagaaga cgatgctccc ccgcacaaaa
     1681 aaatctatgc gggagcttta attttaaggg tgaataatat ttactttctt tgtttcaatt
     1741 gttttgtctc ttacttttaa tgatactaca tagatacctg gaaataacaa ttgagttgaa
     1801 accactttct gatcaatctt tcgatcaaga attatttttt taatgacctg gccttggatt
     1861 gatgatattt caattgatgc agtggaaacg aattcatcta aaagcttttc aagattataa
     1921 acaactatta tatagctgtt tgcgggattg ggataaatct taagataatc attctgttgt
     1981 gatggattca ttttgtgttc tttctttatc ctttctgatt tcaattcatc agaatataga
     2041 taaggttcgc tgtattttac cctaccaata gataaaagca tattccgtgc ataggacttt
     2101 ataggttcat gagcttcttc cactaaagcc atcaatgcta ctgtttgaat actatctgct
     2161 tgcataccgc acagggtgtc gtttactatg tttttaagga tggctgcgta tgaattgtat
     2221 tcggtataaa cagtttgttg ctgtggcgat aattcaaaat caaccggaat agattccagc
     2281 gtattaaatg catcaaccgt atcaccgtga tatatttgct gagttgcaag caagtattta
     2341 gcttcaagcg tttgttcatt ttgtagaaaa gtggtcaggc tatcaattaa actaactgta
     2401 gtatcagtca tcttagatgc aagcaaataa tcaaataatt gagccctttc taatttcaac
     2461 gaggccagtt ccatttcctt aacttcgaga ggagaataaa cggattcacc tgctaagatt
     2521 tggttgtaca tatcttcagg cataggatca atccttttat ccaattcatt gagaacagat
     2581 tcgctgccag cagattgtgg gttggctact aatatatccc tgatcattac atttggtaag
     2641 acaaactcgt tggatactgc tgtttgcatt gcagtatcgc tcaaataagg tgatttgctc
     2701 aagagattat ctctcagttc aagcgcttca ggagggacac tattaacgat attttcagtg
     2761 atttcttcag tgctgcctcc atcaacaaga gtataaagtt cagattcctt ttgttctatg
     2821 gttccattta tggtatgtag atattcatta gcttcttcaa ttgggatgcc attaccatta
     2881 ctaagttttg aagggcaaga agttggttca taataagcat aaataacctg ctttatatag
     2941 acatttgaac aaaattgggt ctcaggtttc aatcttttat tagatgtatt tggttgatgg
     3001 taatacttaa accttgcacc ttggttttga aggtcatacg tttttatcat acttcgttgc
     3061 gaaaatgtat taccagcagg tcctgtttca gaaagagaat caccttgata aagagcaatt
     3121 ccccaatttt gattttgttg gctaaggaat accacctcgt cagatttatt cccggtaaaa
     3181 tcattgcatt tgcaaacaag tcctgtaatc ccattgctac ttcgattgat attctgggct
     3241 ttaatacctt cctgaaggaa cgacaatata ttattgtata ccagattatc cgctgtacct
     3301 gaattattaa ttataattcc cgtctcgtat gtgtttgatc cattataatt acctgtaata
     3361 gtgttttctt gcaatgagta tccggtagaa ttatcaagat aaataccaca tgagggtatt
     3421 gtttgtgtcg ctatcttcgt atgtatagta tttctagtta tttccgggac attaatacca
     3481 ctcaaataca cacttctgag attgtttgag aataagcagt catcaatttt gatatatttt
     3541 aatactccgc tgcaaatagc tttaatacca tatttcaggc cgttaaattc gctgtgaata
     3601 gtatcatcac agggaataac attgctgtgg cacaggctat ttacgccaaa gccactgtta
     3661 taagaataaa taccagtaat tgaatcagct aaag
//
LOCUS       DAOPYB010000259         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_33286_length_3693_cov_11.030511, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000259 DAOPYB010000000
VERSION     DAOPYB010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33286_length_3693_cov_11.030511"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1724)
                     /locus_tag="PKH58_12670"
     CDS             complement(<1..>1724)
                     /locus_tag="PKH58_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNX89926.1"
                     /translation="SDKLPELPERDKEIIENLSRAGQRMMGFCRTNFFKRLDSSGISY
                     LISLYRHVLRNAIYIYALENKLPLPIGDESNFTDELLEDEDIENEIFESNNVQLVKKP
                     DDAQFTFSTSPEDYLSVARDYYNSIAGNSNNVRWLDSKYFKRTLKQALKNDCDRLLEI
                     IKLCGVWKAKKDEKLNTLFQLLNEKHKDEKIIIFTQYSDTAKYIHTQLRRRGITHLEC
                     VTGASANPTHIVERFSPVSNKVTPVPADNDQLRVVVSTDVLSEGQNLQDAHIVINYDL
                     PWAIIRLIQRTGRVDRIGQQSENVLCYSFFPAEGVERIIRLRQRLTDRINENADVVGS
                     DEIFFEGNSQNLMDIYNEKNGVLDDDDEGEVDLSSKAYQIWKNATDANPELKTIIPAL
                     SNVVYSTKSTDQPINEGVITYAKTPSDNDMLLWMDKKGEIKTYSQSAILKALACSIHE
                     PALERTDNHHELVGKSIDQISSQEIKVSGTLGKRFSTKYRIYTLLEAFCKETANGLFV
                     TEDLKLAMDDIYNYPLKENARILLNRQLKKGTSAMEIVDLVKELRNEEELSIKKDEEE
                     TRNKEPQI"
     assembly_gap    1725..1824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1825..3200)
                     /locus_tag="PKH58_12675"
     CDS             complement(<1825..3200)
                     /locus_tag="PKH58_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNX89927.1"
                     /translation="MAQIYDNISTQFTEGLKGIISNTQVKRVDFCVGYFNLRGWGLII
                     NEIDALDGDFVYENDDNIHRICRLLIGMQRPGIEIIKRYINSQTNQIPDSDEVKLCKR
                     QIADDFRKQLIIGKQTNYDEVNLRRLSAQLKAKRVCIKLYLKEPLHAKLYLAHRPEDN
                     FNKIQAIMGSSNLTYGGLNGQGELNAGFTDSDHVEKLANWFDDRWEDRLSLDITEELA
                     EIIDNSWASEKLIPPYYIYLKTAYHLSREARNSISEFSLPREFKKDLFPFQETAVKLA
                     ARHLERRDGAMIGDVVGLGKTITACAIAKVYELNNSCSTLILCPANLQTMWKKYVIEY
                     DLKADIHSLSKPIDEANVRHYDLVIIDESHNLRNSEGKRYQNIKRLINLQNCKVLMLT
                     ATPYNMDFSDLGNQLRLFLKEDTDLGIQPENYIRSLGGEREFSKQHDEDYIRSIKAFC
                     RSNEPDDW"
BASE COUNT          911 a          779 c          675 g         1228 t
ORIGIN      
        1 ataatttgag gttctttatt gcgtgtttct tcttcgtctt tcttgatgga taattcttct
       61 tcgtttctca gttcttttac caaatcgaca atttccatgg ccgaagttcc tttcttcagt
      121 tgccgattga gcaatatccg ggcattttct ttcaggggat aattgtaaat atcgtccata
      181 gccagtttta aatcttcggt cacgaacaat ccgtttgctg tctctttgca aaatgcttcg
      241 agtaaggtat aaatccggta tttcgtacta aagcgtttgc ccagtgtgcc gcttactttt
      301 atctcctgag agctgatctg gtcgatggat ttccccacca attcgtggtg gttatctgtt
      361 ctttccagcg caggttcgtg gatagagcaa gccagtgctt tcagaatagc actttgcgaa
      421 taggttttta tttcaccttt tttgtccatc cacagcagca tatcgttatc gcttggcgtt
      481 ttagcatagg ttattacccc ttcatttata ggctgatcgg tgcttttagt ggaataaacc
      541 acattgctca atgcgggaat aatcgttttc agttcgggat tggcatcggt tgcatttttc
      601 cagatttgat atgctttgga acttaaatcc acttcccctt cgtcgtcatc atccagcact
      661 ccgttctttt cgttgtatat gtccatcagg ttttgagagt taccttcaaa gaaaatttca
      721 tcactgccta ccacatcggc attttcattt atcctgtcgg tcagtctttg tcgtaaacga
      781 atgatgcgtt caactccttc agcaggaaag aatgaatagc aaagcacatt ttctgactgt
      841 tgaccaatac ggtccacacg tccggttcgt tgtataagtc ggataattgc ccagggcaag
      901 tcgtaattaa tcacaatgtg agcatcctgc agattttgtc cctcactcaa tacatcggta
      961 gaaaccacca ctcttaactg gtcgttatca gccggaactg gagttacctt attgcttacc
     1021 ggtgaaaatc gttcaacaat atgagtagga ttagcacttg ctcccgtcac acactccaga
     1081 tgagtaatgc cgcgtcttct gagttgagta tggatgtatt ttgcagtatc ggagtactgt
     1141 gtgaaaatga ttatcttctc atccttgtgt ttttcgttta gcagttggaa taatgtattc
     1201 agcttttcat ctttcttagc tttccatact ccgcataatt ttattatttc gagcagcctg
     1261 tcgcagtcat tcttcagcgc ttgtttcagg gttcgtttga agtatttact gtcaagccac
     1321 cttacattat tagaatttcc ggcaatggaa ttatagtaat ctctggcaac agaaagataa
     1381 tcttcgggcg aggttgagaa tgtaaattgt gcatcatccg gttttttcac caactgcaca
     1441 ttattgcttt cgaagatttc gttttcaatg tcttcatctt ccaacagttc atccgtaaaa
     1501 ttactttcat caccgatggg taacggcagt ttattttcca gtgcataaat gtaaatggca
     1561 ttgcgcaata cgtgcctgta aagcgaaatg aggtatgaaa tcccgctgct gtccagtcgt
     1621 ttgaaaaaat tggtacggca aaatcccatc attcgctgac cggcacggct gaggttctct
     1681 attatttcct tatctctctc cggcagttcg gggagtttat cactnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnttccag tcatccggtt cattgctccg gcagaatgcc
     1861 ttgatacttc ggatataatc ttcgtcgtgt tgtttggaga attctctttc accacccaac
     1921 gaacgaatgt aattttcagg ctgaattcca agatcggtat cttctttcag aaataatcgt
     1981 aactgattgc ccaaatcaga aaaatccata ttatacggag tagccgtaag catcagcact
     2041 ttgcaattct gcaaattgat cagccgcttt atattctgat accgtttgcc ttcgctgttc
     2101 ctaaggttat gactttcatc tatgattacc aggtcgtaat ggcgaacatt tgcttcgtca
     2161 atcggtttgg ataacgaatg tatatccgct ttcaaatcat attctatcac gtatttcttc
     2221 cacatggtct gcaaatttgc gggacacaga attagtgtag agcaagagtt atttaattcg
     2281 tacacttttg ctatggcaca agctgtgata gttttcccaa gtcccaccac atcgcctatc
     2341 atggcgccat cccgtctttc caggtgtcgg gctgcgagtt taacggctgt ttcctggaag
     2401 ggaaacaagt cctttttaaa ttctctgggt aaactgaatt cgctgatgct gtttcgtgct
     2461 tcccggctca gatgatacgc cgttttcaga taaatataat agggtggaat tagcttttcg
     2521 ctggcccagc tgttgtcaat tatttctgcc aattcttcgg taatatccaa actcaatcgg
     2581 tcttcccaac ggtcatcaaa ccaatttgcc agcttttcta catggtcgct atcagtaaat
     2641 ccggcattta attcaccctg cccgttcagt ccaccgtagg tgagattgct gctgcccata
     2701 atggcctgta tcttattaaa gttatcttcg ggccggtgtg ccagatataa ttttgcatga
     2761 agaggttctt tcagatacaa cttgatgcac actcttttag cttttagttg agcagaaagt
     2821 cgtctcaggt taacttcatc gtagtttgtt tgcttaccaa tgattaactg ctttctaaaa
     2881 tcatcagcta tttgcctttt gcacagtttt acttcgtcac tgtccgggat ctgattggtt
     2941 tgagaattga tatatctttt tattatttca ataccaggcc gttgcatccc tataagcagg
     3001 cgacagatac gatgaatgtt atcatcattc tcataaacaa aatctccatc cagcgcatcg
     3061 atttcgttta taatcaatcc ccaaccacgt aaattaaaat atcccacaca aaagtcaact
     3121 cttttaacct gtgtgttgga tattataccc ttcagtcctt cagtaaactg ggtacttatg
     3181 ttgtcgtata tttgtgccat ataatattat ctgtttgttc ttacttgttt tcctctttat
     3241 attcttttac ttagctgata ctcttctcat gacaagttat agtttcatct ccattttcgt
     3301 ctaaattaat atcgtcccac aaaactacaa atttacttta acaatccaat ttaaagtgaa
     3361 taaataaaat gaaaagtaaa cagatatttc ccttaatact tcttgtttta ggatgagctt
     3421 gcaaaaatac aatgctgttt tgacatcttc tgacgcaaaa aaataatttc agaatatttc
     3481 gtgtgggagg ttgataatat ggtccaatat tcaaggattt gtgacttcga cctggctcag
     3541 tcacctatag gccctatgta atgataaaaa tgcgtgtcac aagaattaaa atccgcaaat
     3601 taaaaaacaa tgccgccccg gcggggcttt ttttggtggg gggggcaatc gattttctat
     3661 cctaatgtcg cacctctggt gctgaaaatg aag
//
LOCUS       DAOPYB010000260         3667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_33630_length_3667_cov_9.520210, whole genome shotgun sequence.
ACCESSION   DAOPYB010000260 DAOPYB010000000
VERSION     DAOPYB010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33630_length_3667_cov_9.520210"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PKH58_12680"
     CDS             complement(<1..686)
                     /locus_tag="PKH58_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNX89928.1"
                     /translation="MDRRNFLRAVALGGAAFAIKDTDAMSIMTQSFAADGRIATKYDL
                     VAVMGGEPEVMFRKAIAEMGGMAKFIKKGDKVVVKPNIGWDKAPELAANTNPKLVAEI
                     IKQCFSAGAKEVTVFDHTCDDWRKCYKNSGIEAAAKAAGAKVVPADQGSYYKQVSIPN
                     GKKLTKAQIHEAIVDCDKWINVPILKNHGGAQLTMSMKNYMGIVWDRGFFHANDLQQC
                     IADVCTYEKRP"
     gene            complement(714..>1334)
                     /locus_tag="PKH58_12685"
     CDS             complement(714..>1334)
                     /locus_tag="PKH58_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX89929.1"
                     /translation="VKAIVSKGYRNPTIREMYMFPSQNPGLLPERLMNYELSLLQNLL
                     ENRLILGVNLFRSAGDNMIQTIITNGKPLNVNTGKIDNAGLEMSVACRLNNRIYLTTN
                     YSYLNMKYKITGAPEHKLYAGGSYTTRRWTFSTGVQFIQGLFTSTGQNPQKENFTLWN
                     ARLNYKATNRLSFLLKSENLLNQKYEINAGYPMPGTTIFGGLQLNY"
     assembly_gap    1335..1344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1345..2608)
                     /locus_tag="PKH58_12690"
     CDS             complement(<1345..2608)
                     /locus_tag="PKH58_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX89930.1"
                     /translation="MDYLKRILFFILFAVHIPGGMLAAGLSDLDTVRLDEVIITGSIP
                     KVNLRSLPMSISVVSDRQIRERLQSSVLTVLTEEVPGLFITQRGVAGYGVAAGAAGGM
                     NIRGIGGAPSTGVLVLIDGHPQYMGLMGHPLADSYQSVMTERVEVVRGPASVLYGSNA
                     MGGVINIITKKQKQDGSNLSFQSMYGSYNTLETQAGAGIKRGKLSGNVGMAYNRSDGH
                     REDMDFEQMAVYARTGYELSNHWSTSADINGTKTFTSNPGTINSPVHDNDADVRRATA
                     SLVLENDYKKTSGGLRMYYNLGEHLINDGYRDGAAPPDYRFHSDDYMAGASLYQSYRL
                     MAGNRTTAGVDYQRFGGKAKNVFTGKEVLLADKSINDAAAYLNIQQSLLADKMTLNAG
                     IRYDYNEQSGSEWIPQFGVSYLPVASTVV"
     gene            complement(2596..2997)
                     /locus_tag="PKH58_12695"
     CDS             complement(2596..2997)
                     /locus_tag="PKH58_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNX89931.1"
                     /translation="MPDIKSFEEVMGRFRQIEFHEHFDMIVAIANGGVVPAAILNQRL
                     NTDLQLLKINLRDAEQKPRYDNPQLLSPVDFDCKGKTILLVEDRIKTGATVNFAISLL
                     KEAKVVKTFAVNGNADYALYNEACFKFPWII"
     gene            complement(3001..3510)
                     /locus_tag="PKH58_12700"
     CDS             complement(3001..3510)
                     /locus_tag="PKH58_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNX89932.1"
                     /translation="MDTTVKLYSLKLSNARTYLFAVLFVAGNLLLPQLAHLIPGGGLI
                     FLPIYFFTLIGAYKYGINVGLLTAVLSPLVNSLLFDMPVLAVVPAIMFKSAILAVAAA
                     LAAKYFGKVSLWGILIAILAYQLIGTGFEWVLVRDFTVAVQDFRIGFPGMLIQLFGGY
                     LVLKALQKV"
BASE COUNT          957 a          879 c          778 g         1043 t
ORIGIN      
        1 ggacgttttt cgtaggtgca cacatcagca atgcactgct gaaggtcgtt ggcatggaaa
       61 aatcctctgt cccacacgat gcccatgtaa tttttcatcg acatggtaag ctgcgcgccc
      121 ccgtgatttt tcagaatagg cacgttaatc catttatcac aatcaacaat ggcttcgtga
      181 atttgcgctt ttgtgagttt tttaccgttg gggatcgaaa cctgcttgta atacgagccc
      241 tgatcggcag ggacaacttt ggctccggcg gctttggcgg ctgcttcgat gccgctgttt
      301 ttataacatt tcctccagtc gtcgcaggta tggtcaaaca ccgttacttc tttggcgccg
      361 gcactgaaac attgtttgat aatttccgcc accagtttgg gattggtatt ggctgccagt
      421 tcgggggctt tatcccaccc gatattgggc ttcaccacca ccttgtctcc tttttttata
      481 aatttggcca taccacccat ttctgcaatg gctttacgga acatgacctc cggttcaccg
      541 cccatcacgg ctaccagatc atatttagtt gcaatccggc catcggcagc aaatgattgt
      601 gtcataatgc tcatggcgtc ggtatctttt atcgcgaagg cagcaccacc cagtgccaca
      661 gctctcaaaa aattgcgtct gtccatatta gtaatgtttt aatgtatatt cgttcaatag
      721 ttaagttgta atcctccaaa aatggttgtt cccggcatgg ggtaacctgc atttatttca
      781 tatttctgat tgagtagatt ttcgcttttc agcagaaaac tcaaacggtt tgtcgcttta
      841 taattcagtc gggcattcca tagcgtgaaa ttttctttct gcggattttg cccggtgctt
      901 gtgaataaac cctgtataaa ctgtacgccc gtggagaaag tccatcgacg ggttgtgtag
      961 cttccgcctg cgtagagttt atgttccggc gctccggtaa tcttatattt catgttcagg
     1021 tagctgtagt tggttgtcag gtaaatacga ttgttcagcc tgcatgccac actcatttcc
     1081 agccctgcat tatctatttt tccggtattt acattcagcg gtttaccatt ggttataatg
     1141 gtttgtatca tattatcgcc tgcactccgg aaaagattca cccccagtat cagcctgttc
     1201 tccaacaggt tttgcaaaag ggacaattca taattcatca gtcgttcggg cagtaaaccg
     1261 ggattttgcg atggaaacat atacatctcg cggatggtgg ggtttcggta tcccttgctt
     1321 acaatcgcct tcacnnnnnn nnnntcacta ctgtagatgc aaccggaaga tagctcacac
     1381 caaactgtgg tatccactca ctgccgctct gctcattgta atcataacgt atccctgcat
     1441 tcagggtcat tttatcagcc agcaacgatt gttgtatatt cagataagct gctgcgtcat
     1501 tgatactttt atcggccagc agcacttctt tgccggtaaa tacattcttt gcctttccgc
     1561 cgaaacgctg ataatccact ccggcagttg tcctgttgcc cgccattaac ctgtacgact
     1621 gatacagaga tgctccagcc atataatcgt cggagtgaaa acggtaatca ggcggcgctg
     1681 cgccatcgcg gtaaccatca ttgatgaggt gttcgcctaa attatagtac attctcaagc
     1741 caccgcttgt ttttttatag tcattttcga gtacgagcga agccgtcgca cgacgtacat
     1801 cagcatcgtt atcatgaaca ggactattta tggttcccgg attggaagtg aaggtttttg
     1861 ttccgttgat atcagcggag gtactccagt gattggataa ttcatagccc gtccttgcgt
     1921 aaacagccat ctgttcgaaa tccatatcct cccggtgtcc gtcggagcgg ttgtatgcca
     1981 taccgacatt accactcagt tttcctcttt ttattcctgc acctgcctgc gtttcgagag
     2041 tattgtagct cccgtacatg gactgaaacg acaggttgga accatcctgc ttctgttttt
     2101 ttgtaatgat attgataact ccgcccatcg cgttggaacc ataaaggacg gaagcgggtc
     2161 cccgaaccac ctctaccctc tctgtcatca cagactggta gctgtcagcc agcggatgcc
     2221 ccatcagccc catgtattgt ggatgtccgt cgattaatac cagtactccg gttgaaggcg
     2281 ctcctccgat accgcgaatg ttcattcctc cggcagctcc ggcagcaacg ccataaccgg
     2341 caacaccccg ctgggtaata aaaagcccgg gaacttcctc tgtcagaaca gttaataccg
     2401 acgactgcaa ccgctccctg atctgccggt cgctgacaac cgatatactc atgggcaggc
     2461 ttcgcagatt aactttgggt atggagcctg taatgatgac ttcgtccaga cgcacagtat
     2521 caagatcact taaaccagct gccagcattc ctcccggaat atgtacagcg aagagaatga
     2581 aaaagagtat tcgtttcaga taatccatgg aaatttaaaa cacgcttcgt tgtaaagtgc
     2641 ataatccgcg ttcccgttta ctgcaaatgt ctttaccact ttggcttcct tcagcaaact
     2701 gatggcaaaa ttgacagtgg caccggtttt aatacggtct tccaccagca aaatagtctt
     2761 tcctttgcaa tcaaagtcaa caggcgaaag gagctgcgga ttatcgtaac ggggcttctg
     2821 ctcggcatcg cgcagattaa ttttaagtag ctgtaaatcg gtgtttaaac gctgattgag
     2881 tatggcagca ggaacaacac ccccgttagc gatggctaca atcatatcaa aatgttcatg
     2941 aaattcaatt tgacgaaaac gccccatcac ttcttcaaaa gatttgatat ccggcatttt
     3001 tcaaactttt tgcaatgctt taagcaccag ataaccgcca aagagctgaa taagcatacc
     3061 gggaaaaccg atgcggaaat cctgaacagc aactgtgaaa tcccgaacca gtacccattc
     3121 gaaacctgta ccgattaact gataagccag aatagctatc agtattcccc acagcgatac
     3181 tttgccaaag tattttgctg ccagcgcagc ggcaacggca agaatagctg atttgaacat
     3241 gatggccgga actaccgcta aaacgggcat gtcgaacaac agactattta ccagcggcga
     3301 caggactgca gtgagtaaac caacattgat cccgtatttg tatgcaccga tgagtgtgaa
     3361 gaagtaaatg gggagaaaaa tcaaaccgcc accgggtatc aaatgtgcca gttgaggtaa
     3421 aagcaaatta ccggcaacaa agagaactgc aaatagatag gttcgcgcgt tactgagttt
     3481 cagagaataa agttttactg ttgtgtccat aatattattt tattgattat ttgacaaaat
     3541 atttttattc agaatgttga ttacaaagct ataaaaactt tagaaacatt cagaggatga
     3601 tgcctgattt tataaatatt taagcagata aaaacactta atgattaatt tgaaacttaa
     3661 tgcttat
//
LOCUS       DAOPYB010000261         3617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_34330_length_3617_cov_13.387423, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000261 DAOPYB010000000
VERSION     DAOPYB010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34330_length_3617_cov_13.387423"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3295
                     /locus_tag="PKH58_12705"
     CDS             <1..3295
                     /locus_tag="PKH58_12705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX89933.1"
                     /translation="YKNIVSADKPYFQETKISKEYKGFRSFIQEKDYHQNYIKVKEEH
                     RLKREQLERENLKPVNERVSQRWNNFPAGIRSAFYVAWDPQSLISLKSNIHNLNLIIP
                     EWFFIDPKGDSVSFNIDTVGYNYMRKSGVMIMPMLSNNFDREFRSEGIGRILHDQTKR
                     EKLISTVLNECIRRRFIGINIDLEDLHENSDTYLIQFVREIAAAFHTRGLLVTQDIMT
                     DNTDYNVKELAKYNDYLILMAYDEYSGGSDPGPISSQRWIESEVEQLAKDVPAEKIIL
                     GLGAYGYDWDKTNPDNTRNLTYQQALSLAIASKSNIIFDNDTYNLKFSYKDLSNNKEH
                     QVFFTDAATLFNVMRFAAESEIAGTALWRLGSEDFRIWNFYNKDIGGISPKGFNFKMF
                     ETVSANRGGSLKPDYIGDGEVLDVLYTPRDGKISIELDSVDMLISEEKYLQIPSTFEV
                     QKYGQENDKELVLTFDDGPDATFTPQIIDILSGHHVPAAFFVVGLQAEKNLPILKRLS
                     REGFEIGNHTFTHDNIAKVSPKRALLELKLTRLLIEAVTGRSTILFRAPYNADSEPTT
                     AEEIIPVAMARRENYLDIGENIDPEDWQPGITADTIYTRVVKAVNEKRGNIILMHDAG
                     GETRKETVKAAKMIIEHFQKEGYKFISLADYLQKKKSDLMPPIQKGKGYYIIQFNLLL
                     ASIVYWLGNFLASLFIVFIVLGLARLVSMIVLMIRERAREKNVDYEAVKHRKEYPLVS
                     IIVPAYNEEVNAVSSLNNLLKQTYPNFNIVFVDDGSRDETFKRVSEAFAGNGKMKIYT
                     KPNGGKASALNFGIEHTDAEYVVCIDADTKLYSNVLELLVSHFLAPDADPKLAAVAGN
                     VKVGNQVNMLTKWQSIEYITSQNFDRMAYANINAITVVPGAIGAFKRSAMEVAGKFAT
                     DTLAEDCDLTMRFLRAGYVVGNENKAIAVTEAPETTKQFIKQRSRWTFGVMQSFWKHR
                     DTLFRKQYGGLGLWAIPNMLLFQFIIPFFSPLADLFMIFGLFSGNAGRILLYYLIFML
                     VDASVSIAAFIFEKEKLTKLFWIIPQRFVYRWIMYWVLFKSFKKAIKGELQTWGVLKR
                     TGNVAEVTE"
     gene            complement(3447..>3617)
                     /locus_tag="PKH58_12710"
     CDS             complement(3447..>3617)
                     /locus_tag="PKH58_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNX89934.1"
                     /translation="FPDFAAANVFYKGVALFAGAQMGGILQGTSKPCVLTSRSESSLS
                     KFYSIAVACILA"
BASE COUNT         1034 a          776 c          882 g          925 t
ORIGIN      
        1 ttacaagaat atcgtttcgg ctgataaacc ctattttcag gaaacaaaaa tctcgaagga
       61 gtataaaggt ttccgcagtt ttattcagga aaaggattat catcagaatt acatcaaggt
      121 gaaggaggag caccggctca aaagggagca gctggaaagg gaaaacctca agcctgtaaa
      181 cgagcgcgta agccagcgct ggaataattt tcctgccggt atccggtccg cgttttatgt
      241 ggcctgggat ccgcaatcgc ttatttcgtt aaagagcaat atccacaatc ttaatctcat
      301 aatccccgaa tggtttttta ttgatccgaa aggagattcg gttagtttta acattgacac
      361 cgtgggttat aactacatgc gaaaaagcgg tgtgatgatt atgccgatgc tgagtaacaa
      421 ttttgaccgg gaattccgtt ctgagggaat tggacgaatt ctgcacgacc aaacgaaacg
      481 cgaaaaactg atcagtacgg tgttgaatga atgtatccgg cgaagattta tcgggattaa
      541 cattgacctg gaagatttgc atgaaaacag cgacacatac cttattcagt ttgtcagaga
      601 gattgccgct gcgtttcaca caaggggatt actggtgaca caggatatca tgaccgacaa
      661 taccgattac aatgtaaagg aattggcaaa atacaacgac tacctgattt taatggccta
      721 cgatgagtat tcaggcggca gcgatccggg acccatttcg tcacagcgct ggatagaatc
      781 ggaagtggag caactggcaa aagatgttcc cgctgaaaag ataattctgg gacttggtgc
      841 ttatggttac gactgggata aaacaaatcc cgacaatacc agaaatctga cataccagca
      901 ggccttaagt ctggctattg ccagcaaatc gaatatcatt tttgacaacg acacctataa
      961 tctgaagttt tcttacaagg atttatccaa taacaaagag catcaggtgt tctttaccga
     1021 tgccgctacc ctgtttaatg taatgcggtt tgcagcggaa agtgagatag ccggtacggc
     1081 gctctggaga ctgggaagcg aagatttcag aatatggaat ttctacaata aggatattgg
     1141 cggcatttct cccaaaggtt tcaactttaa gatgtttgaa actgtcagcg ccaacagagg
     1201 cggctcgctc aagccggact acataggcga tggtgaagtg ctggatgtac tctacacacc
     1261 acgcgatggt aaaataagca ttgaacttga ttcggtggac atgcttattt ctgaagagaa
     1321 atacctgcaa atacccagca ccttcgaagt tcaaaaatac gggcaggaaa acgacaaaga
     1381 gcttgtactg acctttgacg atggtccgga tgccactttt acaccgcaaa tcattgatat
     1441 cttatccggt catcatgtcc ctgccgcttt ttttgtggtg ggattgcagg cagagaaaaa
     1501 tctcccgata ttgaagcggc tttcccggga aggttttgaa attggtaacc acacatttac
     1561 ccatgacaat attgccaaag tatctccaaa gagagctttg ctggaactga agcttacccg
     1621 gttacttatc gaggcggtca cgggaaggag taccattttg tttcgtgcgc cctacaatgc
     1681 cgacagcgaa ccgactaccg ccgaagaaat cattcccgtg gcaatggccc gacgggagaa
     1741 ttacctggac attggcgaaa acatagaccc ggaagactgg cagccgggca ttaccgccga
     1801 cactatttac acccgggtgg ttaaagcggt gaatgaaaaa cgtggaaaca tcattctgat
     1861 gcacgatgcc ggtggtgaaa cgcgtaaaga aaccgtgaaa gccgcgaaaa tgattatcga
     1921 acattttcag aaggaaggat acaaattcat ctcgctggcc gattatcttc aaaagaaaaa
     1981 atccgacctt atgcctccca tacagaaagg gaaaggctat tatatcatac agtttaacct
     2041 gttgctggca tctatcgtct attggctggg taattttctt gcctcgcttt tcatcgtgtt
     2101 tatcgttctc ggactggcgc gtttggtttc catgattgtg ttgatgatac gtgaacgcgc
     2161 gagggaaaaa aacgttgact atgaagccgt gaaacaccga aaggaatatc cgttggtttc
     2221 catcattgta ccggcataca acgaggaggt gaacgccgta tcttcgttaa ataacctgct
     2281 gaaacagact tatccgaatt tcaacatcgt gtttgtggat gatggaagca gggacgaaac
     2341 tttcaaacgc gtttcggagg cttttgccgg taacgggaaa atgaaaatct acaccaaacc
     2401 caacggtggt aaagcctcgg cactcaattt cgggattgaa cacaccgacg cggaatatgt
     2461 ggtttgcatt gatgctgaca ccaagcttta ttccaatgta ctggaactgc tggtgagcca
     2521 tttccttgct ccggatgccg atccgaaact ggctgcggta gccggaaatg tgaaagtggg
     2581 caatcaggtg aacatgctta ctaaatggca atccattgaa tacatcacca gtcagaattt
     2641 cgaccggatg gcatacgcca atatcaatgc cattaccgta gtgcctggtg ccattggcgc
     2701 tttcaaacgc tcggcgatgg aagtggcagg aaaattcgcc accgacaccc tggctgaaga
     2761 ttgcgacctc accatgcgtt ttttgcgggc aggatatgtg gttggcaatg aaaacaaagc
     2821 catagctgtt accgaagctc ccgaaactac gaaacaattc atcaagcagc gcagccgctg
     2881 gacttttggt gtgatgcaga gtttctggaa acaccgggat acacttttcc ggaagcagta
     2941 cggtggtttg gggttatggg ctatccccaa catgctcctg ttccagttta tcatcccgtt
     3001 cttttcgccg ttggcagact tatttatgat cttcggcctg ttttccggaa atgcaggaag
     3061 aattctgctt tactatctga tttttatgct ggtagatgca agtgtatcca tagcggcatt
     3121 tatttttgag aaggaaaaac tcacaaaact gttctggatt atcccgcaac ggtttgttta
     3181 tcgatggata atgtactggg tacttttcaa aagcttcaaa aaagccatta agggagaatt
     3241 gcagacctgg ggagttctga aacggacggg aaatgtggcc gaagttacgg aataagttaa
     3301 gagaagcctc accccgcgaa ttatttagca atcatgaggc ttatttcagc catcttttcg
     3361 acaagctcag tctggcagct ttgttttgag gttacgcaag aaggggaagt tcgcgatgtg
     3421 tcaatgctgt cgtgttaact ggcgggctat gccagaatac aggcaacagc gatgctgtaa
     3481 aatttcgaaa gagagctttc gcttcgggag gtgagtacac agggttttga ggttccctgc
     3541 aggataccac ccatctgcgc tccggcaaac agtgcaactc ctttgtagaa tacattggct
     3601 gctgcaaaat cgggaaa
//
LOCUS       DAOPYB010000262         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_34781_length_3585_cov_9.232861, whole genome shotgun sequence.
ACCESSION   DAOPYB010000262 DAOPYB010000000
VERSION     DAOPYB010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34781_length_3585_cov_9.232861"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..128)
                     /locus_tag="PKH58_12715"
     tRNA            complement(44..128)
                     /locus_tag="PKH58_12715"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Ser,seq:tga)
     gene            complement(266..1213)
                     /locus_tag="PKH58_12720"
     CDS             complement(266..1213)
                     /locus_tag="PKH58_12720"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNX89935.1"
                     /translation="MNALTKTDFHFDGQTNVYHGKVRDVYTVKNDLLVMVATDRISAF
                     DVVLPKGIPYKGQMLNQIAAKFLDATTDIVPNWKLSTPDPMVTVGLRCEGFPVEMIVR
                     GYLCGSAWRAYKNGVREICGVKIPDGMRENEKFPTPIVTPTTKAEQGLHDEDISKEEI
                     LKQGLATPEEYALLEKYTLQLFERGTQMAAQRGLILVDTKYEFGKRDGKIYLMDEIHT
                     PDSSRYFYAEGYEQRFENGEPQKQLSKEFVREWLMSNGFQGKEGQVVPEMTDEIVKGI
                     SERYIELFENITGEKFEKADIEAISDRIEKNVTDYLKTV"
     gene            complement(1326..2213)
                     /locus_tag="PKH58_12725"
     CDS             complement(1326..2213)
                     /locus_tag="PKH58_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX89936.1"
                     /translation="MKILKNLHPTLQVVARGHVIKIYGNENEVRTLTDNLTRIESYAL
                     NNNNLTEENIIEIVKGNQPNEITFDNLILHGVNGKSIMARSANQQKLVKDFETNDLLF
                     AIGPAGSGKTYTAIALAVRSLKNREIKKIILSRPAVEAGEKLGFLPGDMKEKIDPYLQ
                     PLYDALQDMLPPPKLKEYMENGTIQIAPLAFMRGRTLSDAVVILDEAQNSTTMQIKMF
                     LTRMGLNTKMIVTGDTTQIDLPYQQKSGLIDALEVLKNTKGIARIEFDKNDIVRHKLV
                     QRIVEAYEKAAPRPPKGGV"
     gene            complement(2399..>3585)
                     /gene="glyA"
                     /locus_tag="PKH58_12730"
     CDS             complement(2399..>3585)
                     /gene="glyA"
                     /locus_tag="PKH58_12730"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNX89937.1"
                     /translation="EQVMEAMGSVLTNKYAEGYPGKRYYGGCEVVDESEQLAIDRLKE
                     IFGAEWANVQPHSGAQANAAVFLACLNPGDKFMGLNLAHGGHLSHGSPVNSSGILYRP
                     CEYNLKEETGRVDYDQMEEVALREQPKLIVGGGSAYSREWDYKRMREIADKVGAILMI
                     DMAHPAGLIAAGLLENPVKYAHIVTSTTHKTLRGPRGGIIMMGKDFPNPWEKTTPKGE
                     LKMMSALLDSAVFPGTQGGPLEHVIAAKAVAFGECLQPEFKEYQAQVQKNAKALANAL
                     INRGFKIVSGGTDNHSMLVDLRSKYPELTGKIAEKVLVEADITCNKNMVPFDSRSAFQ
                     TSGIRLGTPAITTRGAKEPLMEEIAEMIETVLSNVDNQEVIAAVRQRVNKTMENYPLF
                     AY"
BASE COUNT          919 a          793 c          722 g         1151 t
ORIGIN      
        1 tataatgaaa cagcctcttt tgcttatttt tgtaaatgtt ttgcggagag agagggattc
       61 gaacccccgg aacctctcag ttcaacggtt ttcaagaccg ccgcgatcga ccactctgcc
      121 atctctccag atgctttctg gtgtcccaaa agcggtgcaa agataatgct atttttgaaa
      181 ttgccaaata tattttcgaa ttaattttcg gaatacatgt caacagattg ataatcagca
      241 atgataaatt ctgttccttg tttcgctaaa ctgttttcag atagtcggtc acattctttt
      301 caatacggtc tgaaatggct tcgatatctg ccttttcaaa tttttcaccg gtaatgtttt
      361 cgaaaagttc aatgtagcgt tcgctgatgc cttttacaat ttcatcagtc atttccggaa
      421 ccacttgtcc ttcttttccc tgaaagccat tactcatcag ccattcacgt acaaattctt
      481 tcgaaagttg tttttgaggt tcaccatttt cgaaccgttg ttcgtagcct tcagcataga
      541 aataacggga agaatcgggg gtgtgtattt cgtccatcag gtagattttt ccatcgcgct
      601 taccgaattc atattttgta tcaacaagta tcaaaccgcg ctgagcagcc atttgggtgc
      661 cacgctcgaa taattgcaga gtgtattttt ccagcaaagc atattcttca ggtgttgcaa
      721 gtccctgttt cagaatttcc tctttcgaaa tatcttcatc atgaagtcct tgttcggctt
      781 tggtggtagg agtgacgatg ggtgtgggga atttttcgtt ttcacgcatt ccatccggaa
      841 tttttacacc gcaaatttcg cgaacgccat ttttgtaagc tctccaggcg cttccacaaa
      901 gataaccacg aacaatcatt tctacgggaa aaccctcaca acgaagtccg acagtaacca
      961 tcgggtcagg tgtggatagt ttccagttgg gaacaatatc agtggtagca tccagaaatt
     1021 tagctgctat ctggttcagc atctgacctt tgtagggaat tccttttggt aatactacat
     1081 cgaaggctga aatacggtcg gtagcaacca taaccagcaa atcattcttt acggtatata
     1141 catcacgaac cttgccgtga tatacgtttg tctgtccatc gaaatggaaa tcggttttag
     1201 ttagtgcgtt catattgtta tgtatctgcc ccctaacccc cccctaaagg ggaaattatg
     1261 agcgttttaa ttttttttaa tatttgtttg agttctcatt ttgaaaaatg tcattcaaaa
     1321 aggatttaaa ctcccccttt aggggggcgg ggggcagctt tctcatacgc ctccacaatg
     1381 cgttgaacca gtttgtgacg gacaatatca tttttatcga attctattct ggcaatacct
     1441 ttggtgtttt taagtacttc cagcgcatca atgagaccgg atttctgctg ataaggcagg
     1501 tcgatctgtg tagtgtctcc ggtgactatc atcttggtat tcagtcccat gcgtgtaagg
     1561 aacattttta tctgcatggt ggtggaattt tgtgcttcat ccagaataac gacagcatcg
     1621 ctcagtgtgc gtccgcgcat aaatgccagg ggagctatct ggatagtacc gttttccata
     1681 tattccttca atttaggcgg tggaagcata tcctgcaaag catcatacaa gggctgcaga
     1741 taaggatcaa tcttttcttt catgtcgccg ggcagaaatc caagtttttc accggcttct
     1801 actgcaggcc gacttagaat aatttttttt atttcccggt ttttaagcga acgaaccgcc
     1861 aaagctatgg ctgtgtacgt tttgcccgat ccggcaggcc cgatggcaaa aagcaaatcg
     1921 ttggtttcaa aatccttcac cagtttttgc tgattggcgc tgcgtgccat aatggatttg
     1981 ccatttactc catgtagtat caggttgtcg aaagtgattt cgtttggctg attaccctta
     2041 acaatttcaa taatgttttc ttcagtcaag ttattattat tcagtgcata actttcaatt
     2101 cgtgtcagat tatcggtcag cgtacgaact tcattttcgt taccgtagat ttttataaca
     2161 tgtccccttg ctactacctg aagagtgggg tgtaaatttt tcaatatttt catattggca
     2221 ttatttaccc cgtaaaaaac tgtcgtgtcg gtgtgttcag acaaagtaaa tatagatgct
     2281 tcgtcagcat caggacatag attaatatgt gttttttctt tcattaatat cagaggcaag
     2341 aagggtaagg agtaagagat aagtgatttc ttacctcttg cttcttacta tctattcttt
     2401 agtatgcaaa tagcggataa ttctccattg ttttattcac acgttgacgt acggcagcaa
     2461 tcacttcctg attatctacg ttggacaaaa cggtttcaat catttcggca atttcttcca
     2521 tcaacggttc ttttgctccc cgggttgtga ttgcaggagt tcccaaccgg ataccggatg
     2581 tctggaaagc agagcgacta tcaaagggaa ccatattttt gttgcaggtg atatccgctt
     2641 caaccaatac cttttcagca atttttccag tcagttcagg atatttggag cgtaagtcga
     2701 ctaacatgga atggttatcg gttccacctg atacaatttt aaatcctctg ttgatcaatg
     2761 cattggccaa tgcttttgca tttttctgaa cctgtgcctg atattcttta aattcaggtt
     2821 gcagacattc gccaaatgca acagctttag cggcaataac atgttccagc ggtccgcctt
     2881 gtgtacccgg aaatactgct gaatccaaaa gtgcagacat cattttcagt tcacctttcg
     2941 gtgtggtttt ttcccaagga tttgggaaat ccttacccat cataataata cctccacgag
     3001 gaccacgaag ggttttatga gttgttgacg ttacaatatg agcatatttt acaggatttt
     3061 cgagcaatcc tgctgcaatc agtcctgcag gatgagccat gtcgatcatc agaatagcac
     3121 ctaccttatc ggcaatttca cgcatgcgtt tgtagtccca ttcgcgggaa taagcggagc
     3181 ctccgccaac aatcagtttc ggttgttcac gcaacgccac ttcttccatc tgatcataat
     3241 ccacacgtcc ggtttcttct ttcagattat attcacacgg acgatacaga attcctgaac
     3301 tgttaacagg tgaaccgtgt gaaaggtgtc ctccatgagc cagatttaat cccataaact
     3361 tgtcacctgg atttagacat gccaaaaata cagcagcatt tgcctgagcg ccggaatgag
     3421 gttggacatt agcccattct gcaccgaaaa tctcttttag tcggtcaata gcaagctgtt
     3481 cgctttcatc aaccacttcg caaccgccat aataacgctt gccgggatat ccttcggcat
     3541 atttattggt aagtacggag cccatggctt ccattacctg ttcac
//
LOCUS       DAOPYB010000263         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_34853_length_3580_cov_9.180993, whole genome shotgun sequence.
ACCESSION   DAOPYB010000263 DAOPYB010000000
VERSION     DAOPYB010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34853_length_3580_cov_9.180993"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKH58_12735"
     CDS             <1..288
                     /locus_tag="PKH58_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89938.1"
                     /translation="LSVGALPNTEAKGMFADDLKLDKFEFVGQPDLMASPAQTSIEGV
                     FVAGTASAPMDIPDAILSAGSASSEAISYLMKNVAPQPPKGGVKVEQVSGC"
     gene            388..2088
                     /locus_tag="PKH58_12740"
     CDS             388..2088
                     /locus_tag="PKH58_12740"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HNX89939.1"
                     /translation="MEKRIGVYICHCGGNISDYVDVEKVRQSVENDDNIVLAKTTMFA
                     CSDANQKDMVDDINNFNLDGVIVASCSPKLHLVTFRNVTERGNLNKYTYVHSNIREQA
                     SWAHSDNKEGATEKAIHLVKSAIAKARHTQPLDPTKIKAEKSVAVIGAGIAGLKAALS
                     LSEKETHVFVIEKEAAVGGWVAKWKDLFLTDETGDAIIKRLYEELKKRQNVTIFLNSE
                     VVGNTGSIGNISLKIKQKEEEFDITVGSVLVATGFEPYKPKEDEFGFASVPNVITLPE
                     FKQLIDHEKDTLTYNGKPVHNIAYIYCVGSRQPEGENTYCSRYCCTATIHAANQVRDK
                     FRDIQNYHFTRGVRSYGKQELLYAESLQGGDIYLQSYEDGLPVVFARNNKPVVRVNDI
                     LTGGKELEVEADLVVLVTGMAPRSDNKVGSLFKLPKGRDKFFNEIHMKLRPVETVIDG
                     VTIAGACQGPKTIIETVNSALSAATKAYSNISKGVLETDPQIAKIEAPACIGCGDCLP
                     ACPFDAISMVKEGEKSIAVINNSLCKGCGMCTPLCPMDAIDLIAYSNVEIMGMIDALV
                     "
     gene            2139..2474
                     /locus_tag="PKH58_12745"
     CDS             2139..2474
                     /locus_tag="PKH58_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNX89940.1"
                     /translation="MKEENIEDKTNLSYYTVEGVAVEKGKTAKYLREKKGVSDKVKEN
                     LKEFTQVKRKITEALKENDLTVAQISEKTGLSGPDTLFYLMSLVKYGIVKVGEIDDMD
                     EYFTYKLNK"
     gene            2474..3517
                     /locus_tag="PKH58_12750"
     CDS             2474..3517
                     /locus_tag="PKH58_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX89941.1"
                     /translation="MATINPKFGKDLKKYGADSFNACYNCGNCTAVCSLSTEDTSFPR
                     ELVRMSVLGLEDELKTSTKPWECYYCGECSTHCPQEANPGELMMSLRRWLTAKYDWTG
                     LSGLFYKYLPATVTAMLLVLAGVIFFAVFVNWELERMLHFGHLFEMIAIATVFVVIFI
                     PNLLRMWYFTVIKSGVKVPFKKYFTTLGELFVHMFTQKRAKDCDDNHFRWLEHLILVF
                     AYLALLFTTVFLNWFGTENLFVIILGYFESAFIFIITVDFVKDRVQKKKALSKKSQPS
                     DWFFVIWLLLMGLTAFLVRLFIDVNILESNKWMYIFHLTVLAQWALIIVPFGKWTHFL
                     YRSFAMYFVKLKE"
BASE COUNT         1050 a          793 c          871 g          866 t
ORIGIN      
        1 ctttcggtgg gcgctttgcc caataccgaa gcaaaaggta tgtttgccga cgatctgaag
       61 ctggacaaat tcgaattcgt gggacaaccc gacctgatgg cctcacctgc ccagacaagc
      121 atcgaaggtg tgtttgtagc cggaaccgcg tcagcaccga tggatatccc cgatgccatt
      181 ctctcggcag gatcagcgtc gtccgaagcc atcagttatt taatgaaaaa cgttgccccc
      241 caacccccta aagggggagt taaagttgag caagtcagtg gctgctaacg ttataataat
      301 aattaatgca attctgtaaa taatataaat actgaaaaaa cgctcttgtg ttacccccct
      361 tcaggggggt aggggcaggt aactattatg gaaaaaagaa taggagtata catttgtcat
      421 tgcggtggaa acatttcaga ctacgtggat gtagagaaag tgcgtcagtc cgtggaaaat
      481 gacgataata tcgtgctggc aaaaacaacc atgtttgcct gctcggatgc caatcagaaa
      541 gacatggtgg atgatatcaa taacttcaat ctcgatggag taatcgtggc atcctgttca
      601 ccgaaactgc acctggtcac attccgcaat gtaaccgaac gcggcaacct gaacaagtac
      661 acgtatgtgc attccaatat ccgcgaacag gcatcgtggg cgcattccga caataaggaa
      721 ggagccaccg aaaaagcaat tcacctggtg aagtcggcaa tagccaaagc gcggcacaca
      781 cagccgctcg acccgaccaa aattaaggcc gaaaaaagcg tggcagtgat cggagccggt
      841 atcgccggac tgaaagccgc attgtcgctt tcggagaagg aaactcatgt gttcgttatt
      901 gaaaaggaag cagccgtcgg cggctgggtg gctaagtgga aagatttgtt tctgaccgac
      961 gaaacgggtg atgccatcat caaacgactg tatgaagaac taaaaaaacg gcagaatgtg
     1021 actatcttcc tcaattccga agtggttggc aatacaggaa gcatcggtaa tatttcactc
     1081 aaaataaaac aaaaggaaga ggaattcgac attacagtgg gctctgtact cgtggcaacc
     1141 ggatttgaac cctacaaacc gaaagaagat gaatttggct ttgccagtgt gcccaacgtg
     1201 attaccctgc cggagttcaa acagttgatt gaccacgaaa aagatacgct tacctataac
     1261 ggaaaacccg ttcataacat cgcatatata tattgtgtgg gaagccgtca gcccgaaggg
     1321 gaaaacactt actgctcacg ttactgctgt acggcaacca tccacgctgc caatcaggtg
     1381 cgcgataagt tcagggatat ccagaattat cacttcacgc gtggtgttcg ctcgtatgga
     1441 aaacaggaat tgctctatgc cgaaagcttg cagggtggcg atatctattt gcagtcttat
     1501 gaagatggac tgcctgtggt ttttgccaga aacaacaaac cggtggtgcg ggtaaacgat
     1561 attctcaccg gaggaaaaga actcgaagtg gaagccgacc tggtggtgct ggttaccgga
     1621 atggctcccc gcagcgacaa caaggtgggt tcattgttca aactgccgaa aggacgtgat
     1681 aaattcttca acgaaataca catgaaactc cgtccggtgg aaaccgtgat tgacggtgtg
     1741 accatcgccg gagcctgtca gggaccgaaa accattatcg aaacggtgaa ctcggcactc
     1801 tcggcggcta ccaaggcgta ttcaaacatc agtaagggag tactcgaaac cgatccgcaa
     1861 atcgcaaaaa tcgaagcgcc ggcttgtatc ggttgcggtg attgtctgcc ggcctgtccg
     1921 tttgacgcga tttcgatggt gaaggaaggt gaaaagtcaa ttgccgtcat caacaattcg
     1981 ctctgcaaag gttgcggcat gtgcacaccg ctgtgtccca tggacgccat tgatctgata
     2041 gcgtactcca acgtggaaat tatgggtatg atcgacgcgc tggtttaagc agttctgagg
     2101 aataaaaaac aataattctg aaaacgaagg aaatcattat gaaagaagaa aacatagaag
     2161 ataagaccaa tctcagctat tacactgtcg aaggtgtggc ggttgaaaaa gggaaaaccg
     2221 ccaaatacct gcgtgagaaa aagggtgttt ctgataaggt aaaggaaaat ctgaaagaat
     2281 ttacccaggt aaaacgtaaa atcaccgaag cgctgaaaga aaatgacctg accgtagcgc
     2341 agatttctga aaagacaggt ttgtcgggac ctgacacgct gttttacctc atgtcacttg
     2401 tcaaatacgg cattgtgaaa gtgggcgaaa tcgacgacat ggatgaatat ttcacttata
     2461 aactgaataa ataatggcaa cgataaatcc taaattcggt aaagatctga aaaaatacgg
     2521 agcagacagc ttcaatgcct gctacaactg tggaaactgt accgcggtgt gttcactttc
     2581 aaccgaagat acctcatttc cgcgtgaact ggttcggatg agtgtactgg gactggaaga
     2641 cgaactgaaa acctctacaa aaccctggga atgctattat tgcggcgaat gcagcacaca
     2701 ctgcccgcag gaggccaatc ccggcgaact gatgatgtcg ttgcgccgct ggctgaccgc
     2761 caaatacgac tggacaggac tttcgggctt gttctacaag tatctgcctg ctactgtgac
     2821 tgccatgctg ctggtgctgg caggggtgat attcttcgcc gtcttcgtaa actgggaact
     2881 ggaacgaatg ctgcacttcg gacacctgtt cgagatgata gccatcgcca ctgtgtttgt
     2941 ggtaatattc attcccaatt tactgcgcat gtggtatttc acggtcatca aatccggagt
     3001 gaaagtgccc ttcaaaaagt atttcaccac gttgggcgaa ctgttcgtgc atatgtttac
     3061 acaaaaacgg gccaaagatt gcgacgacaa tcatttccgc tggctcgaac acctcatcct
     3121 ggtgtttgct tatctggcac tgctgttcac taccgtattc ctcaactggt ttggcaccga
     3181 aaatctgttt gtaatcattc tcggttattt cgaaagtgcc tttatcttta tcatcacggt
     3241 ggattttgta aaagaccgcg tgcaaaagaa gaaagcgttg agcaaaaaat ctcagccatc
     3301 cgactggttc tttgtcatct ggctgctgtt gatgggactt acagcattcc ttgtgcgttt
     3361 atttatcgat gtaaatatac ttgaaagcaa taaatggatg tacatttttc acctcaccgt
     3421 actggctcag tgggcattga tcattgttcc cttcggaaaa tggacacact tcctgtaccg
     3481 ctcgtttgcc atgtattttg tgaaactgaa agagtaaaat ccggattata aatttcaaaa
     3541 aaggagaaac aaaagaagtt tctctttttt tttgttaaaa
//
LOCUS       DAOPYB010000264         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_35067_length_3565_cov_9.002564, whole genome shotgun sequence.
ACCESSION   DAOPYB010000264 DAOPYB010000000
VERSION     DAOPYB010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35067_length_3565_cov_9.002564"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2679
                     /locus_tag="PKH58_12755"
     CDS             <1..2679
                     /locus_tag="PKH58_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX89942.1"
                     /translation="SNPAFVTKDYNGVIEITYDATLGTAGLKDYTGTDGVYAHTGVIT
                     NVSTSDTDWKHAPTWGDNSAKYKLTSLGNNKWKLLITPNMTGYYGLTTGEVVKKLAFV
                     FRNGLKTKEGKDGTVSSPRDIMLTVYDAGLNVGFTNPAADKSVAVGTSETITFTSSIA
                     ANLELFINGSSVATAPSATTLSKSFTYSESKDYQLIAKATAGTEIKYDTVNVNVAAPV
                     QNQPRPAGYKDGITYSADGTQATLIMYAPGKTNVFLIGDMNNWAQKNEYQLKKDGDYW
                     WITITGLTPGKLYGFQYLVDGTMRVSDPYAELVLDPWNDKWINEKFVRYPNLPAYPEG
                     KTIGVVATLQSNKPAYAWEVPNFTMPSHENMVIYEMLFRDFTVEKSIDAALTRLDYLK
                     TLGVTAIELMPIQEFDGNESWGYNPSHFFAIDKAYGTSEKYKKFIDECHKRGIAVILD
                     MVFNHATGNSPFAALYWDGANNRPAANNPWMNPVAPHAYSVFNDFNHSFSGTREHFKR
                     VLKYWITEYKVDGYRMDLAKGFTQYNTADSYDASRIAILNDYYNAAKEAKPDVMFILE
                     HLGVSQEQNEFANTGMYLWGKNNEQYSQAAMGYQENSDFSGMNSSPRQWVGYAESHDE
                     ERNFYKAKMFGAGSMQTDSVYRVSKRVPLNIAFETLIPGPKMIWQFGEMGYDYSINFG
                     GSNVANKPSAWSWLSLAHRKAAYDASSKVITLRKLYPNAFTQGTFSMQIAASDWNQGR
                     RIALTHSDLSMVALGNFNASAPITALPNFPKAGTWYELLSGEVLNVTNTNMTISMNAG
                     ELKIYTDRIVNTGTDVKEVKMDINCTIFPGVTTDKLWISSTTEIGTVNIYNIQGALQS
                     TYSNKSEIDASGLASGIYLLEINTAEGKAIRKFIKK"
     gene            complement(2798..>3565)
                     /locus_tag="PKH58_12760"
     CDS             complement(2798..>3565)
                     /locus_tag="PKH58_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNX89943.1"
                     /translation="ILGAGKMGTFLTDVMCIDHEVALFDTDPKKLRFVFNTYRLTKPE
                     EVKEFEPELMINCVTLKYTIEAFEMILPYLPVNCIISDIASVKTGLQEYYENCGRRYV
                     STHPMFGPTFATLDNLSSQSAIIISESDHMGKAFFKDLYRSLHLKIFEYTFEVHDETI
                     AYSLSIPFASTLVFASVMKPQEAPGTTFKRHMDIAQGLLSEDDYLLSEILFNPNTPKQ
                     VENIRKELASLLDIINDKDTERMQAFLKKVRKNIELK"
BASE COUNT         1118 a          756 c          749 g          942 t
ORIGIN      
        1 tcaaacccgg cttttgtaac caaagattat aatggtgtaa ttgaaataac ttatgacgcg
       61 acactcggta ctgccggact taaagattac accggtacag acggagttta tgctcacaca
      121 ggagtaatta caaatgtaag tacaagcgat actgactgga aacatgctcc aacatggggt
      181 gacaattcag caaaatataa acttacctcg cttggaaata ataaatggaa gttattaatt
      241 acacccaata tgaccggtta ttatggcctt acaaccggcg aagtggtgaa aaaacttgct
      301 tttgtatttc gcaacggact aaaaacaaag gaaggaaaag acggaacagt gtcaagtccc
      361 cgggatatta tgctcactgt atatgatgcc ggtttgaatg tgggtttcac taatcctgct
      421 gcagataaat cagtagctgt tggaacttca gaaacaatca cttttacctc atctattgct
      481 gctaatctgg aattatttat caacggatca agcgtagcca cagcaccatc tgcaacaact
      541 ctttcaaaat cattcactta ctcagagagt aaagattacc agcttattgc caaagcaact
      601 gcaggcactg aaatcaagta cgatacggtg aatgtgaatg tggcagctcc tgtacaaaac
      661 cagccaagac ctgccggtta caaagacgga ataacctatt ctgccgacgg aacacaagct
      721 accctcatca tgtatgctcc gggaaaaact aatgttttcc tgattggtga catgaataac
      781 tgggcgcaga aaaatgaata tcagctgaag aaagacggag attactggtg gattactatc
      841 accggactta cacccggaaa gctctatggt ttccagtatc tggtagatgg cacaatgcgt
      901 gtatcagatc cttatgctga gcttgtactc gacccctgga acgataaatg gattaacgaa
      961 aagttcgtaa gatatcccaa tttacccgcc tatcccgaag gaaaaacgat aggtgttgta
     1021 gccaccctgc aaagcaacaa accggcctac gcctgggaag tgcccaattt cacgatgcct
     1081 tcacatgaaa atatggtgat ttatgaaatg cttttccgcg atttcacagt agaaaaatcc
     1141 attgatgctg cactcacaag attggattac ctgaagactt tgggtgtgac ggccatcgaa
     1201 ctgatgccaa ttcaggaatt cgacggtaat gaaagctggg gatacaaccc aagccatttc
     1261 tttgccatag ataaagccta cggaacttcc gaaaagtata aaaaattcat tgatgaatgt
     1321 cataaaagag gtatagctgt aattctggac atggttttca accatgcgac cggaaacagt
     1381 ccgtttgcag ctctctactg ggatggcgca aacaacagac ccgcagcaaa taatccctgg
     1441 atgaatcctg ttgcccctca tgcttatagc gttttcaatg atttcaacca ttcgttttcg
     1501 ggcaccagag aacatttcaa acgtgtactg aaatactgga ttactgaata caaggttgat
     1561 ggatatagaa tggatttggc caaaggattt acccaataca atacagcaga ttcgtacgat
     1621 gcgtcgcgaa tcgcgatatt aaatgactat tacaatgctg ccaaagaagc caaacccgat
     1681 gtaatgttta ttctggaaca tttaggtgtt agtcaggagc aaaatgaatt tgccaataca
     1741 ggcatgtatc tgtggggaaa aaacaacgag caatattcac aggctgcaat gggatatcag
     1801 gaaaacagcg atttcagcgg aatgaattca tccccccggc aatgggtagg ttatgccgaa
     1861 agtcatgatg aagaacggaa tttttataaa gctaaaatgt tcggtgccgg cagtatgcaa
     1921 accgactctg tttatcgtgt ttctaaacgg gtccctctta acattgcttt cgaaacactc
     1981 atacctggtc cgaaaatgat ttggcagttt ggagagatgg gttacgatta cagcattaat
     2041 tttggcggaa gcaatgttgc caataaacct tctgcctgga gctggctcag tctggcacac
     2101 cgaaaagcgg cctatgatgc cagttcgaaa gtaatcacac tgcgtaagct atatcccaat
     2161 gcgttcactc aagggacatt ttccatgcag atagcagctt ccgactggaa tcaggggaga
     2221 cgtattgcac tcacccactc cgatctaagt atggtagctt tgggtaattt caacgcatca
     2281 gctccaatta cagcgctgcc taattttcca aaagccggaa cctggtacga attacttagc
     2341 ggagaagtgc ttaacgttac caataccaac atgaccattt ccatgaacgc cggcgaactg
     2401 aagatttaca ccgaccggat tgtgaatacg ggaaccgatg tgaaagaagt gaaaatggat
     2461 atcaactgca ctatttttcc tggagtaact accgataaac tgtggatatc ttccactact
     2521 gaaattggaa ctgtaaatat ttacaacata cagggtgcct tacaatctac ttactccaat
     2581 aagtctgaaa ttgatgcttc cggattagct tcaggtatct atctgttgga aattaacacc
     2641 gctgaaggta aagctatccg taaattcata aagaaataaa aataagttgt gtgcgatttc
     2701 tcaaaaaagc cggccacaga aaatttctgt aaccggcttt ttttttgaat gtcgtttaat
     2761 aaaaaaacaa ctgaatattt ccaacaaaca aggttcgtta tttcaactcg atattttttc
     2821 taactttttt caaaaatgcc tgcatacgtt cggtatcctt gtcattaatt atatccagca
     2881 gactggccaa ttccttgcgt atattttcaa cttgtttcgg cgtattggga ttaaacaaaa
     2941 tttccgacaa cagataatcg tcttcggaaa gtaagccctg agctatatcc atgtgccgct
     3001 taaaagtggt acccggagct tcctgaggtt tcattactga tgcaaaaacc agtgtggacg
     3061 caaatgggat agagagcgaa taagcgatgg tctcatcgtg cacctcgaac gtatattcaa
     3121 aaattttcaa atgaagcgat ctatacaaat ccttgaagaa cgctttgccc atatgatcgg
     3181 attcggaaat aatgatagcg ctttgagaac tcaggttatc aagagtggca aatgtagggc
     3241 caaacatagg gtgtgtggag acatagcgtc tgccgcaatt ttcataatat tcctgcaaac
     3301 cagtttttac ggaggcaatg tcggaaatga tgcagttcac gggaagatac ggcaagatca
     3361 tttcgaaagc ctcaattgtg tacttcagcg tgacgcagtt aatcatcagt tccggttcaa
     3421 actctttcac ttcttcaggc ttcgtcagcc ggtaggtgtt aaacacaaac cggagttttt
     3481 tcgggtcggt atcaaaaagc gccacctcat gatcaataca catcacatcg gtcaggaagg
     3541 tacccatctt acctgctccg agaat
//
LOCUS       DAOPYB010000265         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_35851_length_3511_cov_8.771991, whole genome shotgun sequence.
ACCESSION   DAOPYB010000265 DAOPYB010000000
VERSION     DAOPYB010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35851_length_3511_cov_8.771991"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1842
                     /locus_tag="PKH58_12765"
     CDS             88..1842
                     /locus_tag="PKH58_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89944.1"
                     /translation="MKKVLLLCSMFLSLNLFSQGYWTQKADFGGTPKYWAVGFAINGK
                     GYAGLGRGNGFANSSDLWEYNPVTNAWIQKSSFPGGARYSSHVFVLNNRAYFCSGAYW
                     SGISGDYTPYNDVWEYNPATDTWTQKNNFPGPGRHDAFAFSYGGLGYLCFGQDDFQVT
                     LNDLWEYNPTTDTWTQKASFPGTARSGGIQFGFGKYGYIGLGRDINAVSLGDIWRYDC
                     SQNTWTQMNNFPAASRTSASTFVTDFRAYAVCGYNFQTSSYPHELWEYEPNTDSWVQR
                     EDFPPGDRACGTAFSINNIGYFGLGNVYSSFNKDFWEYTPSCTVGVNANATLIGTTIY
                     ALGSGTSYQWLDCNNGFAVIPNETNQSYTPAVSGNYAVIVFQNTCRDTSDCIAVDICD
                     ALNTNVTVIGPTIYSLAAGATYQWLNCDVGLAVIDNETNQSFSPPVDGNYAVIVTQGN
                     CSDTSACISFSAYGIDETENFGILIYANLLQKEIIIDVKAGEVKSDVVLTDMLGRIVR
                     TYHVNSSGLQKFSMGSIPEGFILLALKPIMGKSQKSSSLQNDASCAKFTHRCVILQIA
                     LLTLAFAAITVSDRCAIL"
     gene            1950..3137
                     /locus_tag="PKH58_12770"
     CDS             1950..3137
                     /locus_tag="PKH58_12770"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX89945.1"
                     /translation="MSLIERKISGRLREVMHTSEIVVLTGMRRVGKTTVLKMLYQEIP
                     GKNKVFLDFENLLDRKIFEETDFNRIVTGLKEAGLKNEKGAYVFIDEIQNFPQSVHAL
                     KYLYDHYSIKFFVTGSSSFYLKNLFPESLAGRKTIFELFPLDFQEFLRFKEVLVPFET
                     SFKQKAENKNIIRHEKLSGYVSEYIEWGGFPQVVLASDISHKKRLISDIFSSYYQKDI
                     LQLSDFKNLNAFRDLLMLLIQRTGSKLDISKLASETGISRDTVYSYLSFLEATYMISL
                     IRPFSSNADREVSGSRKLYFCDNGILSVFGKLSEGSMFENAVYCQLRDIEKINYYQKR
                     SGIEIDFVLPVQKIAIEVKSRWNDIDFNKVARVTSEIKLKESYLLTRQFNEHKMCIPF
                     EML"
BASE COUNT         1053 a          606 c          729 g         1123 t
ORIGIN      
        1 ctattatttc tgacaaagat aacatatttc atacttatct tagaatagag ccattttttt
       61 taattttgtg atagatataa accatatatg aaaaaggtat tgttgttatg ttccatgttt
      121 ttgtctttaa atcttttttc acagggatat tggacacaaa aggctgattt tggcggtact
      181 ccaaaatatt gggcagtagg ttttgccatc aatggtaaag gttatgcagg tttgggacgt
      241 ggaaacggtt ttgcaaattc tagtgatctt tgggaatata accctgttac gaatgcctgg
      301 atccagaaat caagttttcc gggtggagca agatactcat cgcatgtatt tgtgcttaac
      361 aatagggcgt atttttgttc cggagcttac tggagtggta tttctggtga ttatactcct
      421 tataatgatg tatgggaata caaccctgca acagatacat ggactcagaa aaataatttc
      481 ccgggtccgg gcaggcatga tgcttttgct ttttcttatg gtggtttggg gtatttatgc
      541 tttggacaag atgattttca agttacgtta aatgatttgt gggagtataa cccaactacc
      601 gacacctgga cacaaaaggc tagttttccc ggtacggcca gatcaggcgg tattcagttc
      661 ggctttggaa aatatggata tattggcctc ggaagagata tcaatgctgt gtctttaggt
      721 gatatctggc gttatgattg ttctcaaaac acctggactc agatgaataa ttttcctgct
      781 gcaagcagaa cttcagcctc aacatttgta actgacttca gagcgtatgc cgtttgcgga
      841 tataatttcc agacatcgtc ctacccgcat gaattgtggg aatatgaacc taataccgat
      901 tcgtgggtgc aaagggaaga ttttcctccg ggtgacagag cttgtggaac agccttttca
      961 atcaataaca taggatactt cggcttaggt aatgtttata gtagttttaa taaagacttc
     1021 tgggaataca caccatcatg cactgttggc gtcaacgcaa atgctacact aattggaaca
     1081 actatttatg cattaggttc tggcacttca taccaatggc tggattgcaa taatggtttt
     1141 gctgttatcc ctaatgagac caaccaaagt tatacgcctg ctgttagtgg caattatgcc
     1201 gtgatcgtgt ttcaaaatac ttgtagagat acatcagatt gtatagctgt tgacatctgt
     1261 gatgctttaa acacaaatgt tactgttatc gggccaacga tctattccct tgctgccggt
     1321 gcaacctacc aatggttgaa ttgcgacgtt ggcttagcgg ttattgataa tgaaacaaat
     1381 caaagttttt cgccgcctgt ggatgggaat tacgctgtta tagtaactca gggtaattgc
     1441 agcgatacct cggcatgtat ttcattcagc gcttatggta ttgatgaaac agagaatttc
     1501 ggcattttga tttacgcaaa cctgttgcaa aaagaaataa tcattgatgt aaaagctggc
     1561 gaggtgaagt cggatgtcgt gctcacagat atgcttggaa ggattgtcag gacatatcat
     1621 gtgaacagtt cgggcctgca aaaattcagt atggggtcca ttccggaggg atttattttg
     1681 ttagctttga aaccaattat gggaaagtca caaaaaagtt cttcgttaca gaatgatgcc
     1741 agttgtgcga agtttacaca ccgttgtgtg attttgcaaa tcgcactcct aacattggct
     1801 tttgcagcaa taactgtttc cgaccgttgt gcgattttgt aaatcgcact ccccctgtga
     1861 ctacggattt gagattttat taaaaaaaat tgtcaatatg tcgattacaa ctcgacaaat
     1921 ttgtgtatat ttgtcgaaaa caattcgaca tgagtttaat tgaaagaaaa atttcaggca
     1981 ggctcagaga agtcatgcat acttccgaaa ttgtggtact taccggtatg cggagggttg
     2041 ggaaaaccac ggtgctgaaa atgttatatc aggaaattcc cggtaaaaac aaggtttttc
     2101 tggattttga aaatctctta gaccggaaaa tttttgaaga aacagatttt aaccggattg
     2161 taaccggatt aaaagaagcc ggcctcaaaa atgaaaaagg cgcgtatgtc tttattgatg
     2221 aaatccagaa ctttccccaa tctgttcatg cattaaaata tttgtatgac cattattcca
     2281 taaagttctt tgttaccggc tccagcagtt tttatctgaa aaatttattt ccggaaagtt
     2341 tagccggaag aaagacaatt tttgaattat tcccgcttga ttttcaagaa tttttgcggt
     2401 ttaaagaagt tttagttccg tttgaaacat cttttaaaca aaaagcagaa aacaaaaaca
     2461 tcatacggca cgaaaaacta agcgggtatg tttctgaata tattgaatgg ggcggatttc
     2521 cacaagttgt tctcgcttct gatataagcc ataaaaaaag actcatttca gatatttttt
     2581 catcttacta tcaaaaggat attctgcaat tatcggattt taagaacctt aatgccttcc
     2641 gtgatttgct gatgttgtta attcagcgca cgggatcaaa gctcgatata tcaaagcttg
     2701 cgtcagaaac aggtataagc cgcgacacgg tatatagtta tctgtcgttt cttgaagcaa
     2761 cgtatatgat ttcgcttata cggccttttt caagtaatgc tgacagggaa gttagcggct
     2821 cgcgaaaatt atatttctgc gacaacggaa tactgtccgt ttttggaaag ctttccgaag
     2881 gcagtatgtt tgaaaatgct gtgtattgcc aattgaggga tattgagaaa atcaattatt
     2941 atcaaaagag aagcgggatt gaaattgatt ttgttttgcc cgttcagaaa attgccattg
     3001 aggttaaatc gagatggaat gatattgatt ttaataaagt agcccgtgtt acatccgaaa
     3061 ttaaactaaa agaatcatat ttgctaacac ggcagtttaa cgaacacaaa atgtgcatcc
     3121 cttttgaaat gctttgaaag ctcgttgtgc gattttgtaa atcgcacact cctcaattca
     3181 cccccgttgt gcgaagtcta cacaccgtta ttcgattttg taaatcgcac tcctcaacat
     3241 aagcttatgc agcaataaag ttttcgccaa ctcagtttcc ttatgtgtat agcatttgaa
     3301 tagtaatacc aaagtgaatt tgtagatagt ccaatgttgc tgagtttatc gaagcaaaga
     3361 cgtactacaa attcttactt tggttatgcc gatataaaaa taaatagtac aaacaattga
     3421 aatgaagttt ggactatatt atttttcaca tcggtataaa tactaaaagt cgtttgaatt
     3481 tatgtgttta gatacaaata gtcgtttgac t
//
LOCUS       DAOPYB010000266         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_36213_length_3487_cov_8.381702, whole genome shotgun sequence.
ACCESSION   DAOPYB010000266 DAOPYB010000000
VERSION     DAOPYB010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36213_length_3487_cov_8.381702"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2670)
                     /locus_tag="PKH58_12775"
     CDS             complement(<1..2670)
                     /locus_tag="PKH58_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HNX89946.1"
                     /translation="MKIKHRIMWLFTFILLSLLLPQWSAAQNNALSFDGTDDYVELGT
                     LAASNPDFSQAVSLTLWVKFNALAESEGMFDIGDNDGIRFFQFTYGGQTSLGYQAWSP
                     VTGGSSVSLSSTQMVTGRWYHFAFTEGNGYVKMYLDGMLVATNPTYSMPNNINRTGNV
                     LGAYYDYIKNLTKYYFNGVLDEVSLWNRELSQTEVYNCIKSGFTGSESGLALYYQFNQ
                     GTANGTNTSVQTLTSKVSSPNISGTLRNFTLSGTSSNWVSGYTSQVVYSITPSTDTIS
                     LGRALNSKAKIDIVSNTKWTVTESSSWLSLDKTSGSFSDSITITATAVNSSNTSRSAT
                     ITLSSTDVSQPVTVVVTQDPVSLTLSSGLDTLDAGQNSTSTVDIVNDGDTWMVVESSD
                     WLSVSPTSGSTDATLTLTAITTNETEADRSAEVIVKGTISGDDTIVVTQLRPTITLSQ
                     NSFRFISPTTSGSVGITTPVAWTATGNASWLTLSSSEGTGNTTINFTAESLPEGTAFR
                     SGEIVFTGAFISDTLHFFQYNETDNALSFDGSDDHIIFDDITASNADFLNGITYSAWV
                     KFDELQYNKYLVYLFPSGTNSYKALYLSGYGNGLHLESYYKSFTINNVLSANVWTHIA
                     FTIKSLGITDSYGIHGTGTIYVNGEEVGSGTVYMPVSSTYDNFTVGNRISDNLITSPY
                     CSMDEIGLWDRVLTQSEIVELMRNGITGSEPDIVTYYNFKQGYSKSDNKDIDVLYSQA
                     ASPSINGELFNFALSGTSSNWVDNYDVSEAACKLTLSDSVLSVESIPGNSKKFAVKSN
                     AAWSISPSESWYTLDSYSGYGNDSITVTAAELNLLADESDTLTVSVNGVNKNLVLSQT
                     YPTIQLSADTLWVCDTEHKDSFTINTNISNWTVT"
     gene            complement(2682..3014)
                     /locus_tag="PKH58_12780"
     CDS             complement(2682..3014)
                     /locus_tag="PKH58_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89947.1"
                     /translation="MKTETSNNAFWVYILQTGKHKEWIISTTLNIEQILNNDFEGNNR
                     NIKLLYCRRFDEMFLALGHKMLLSFLDSKSVRSIVRRNNPLMEDISMKVNVLTTENKL
                     QNQNNKSC"
     gene            complement(3212..>3487)
                     /locus_tag="PKH58_12785"
     CDS             complement(3212..>3487)
                     /locus_tag="PKH58_12785"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX89948.1"
                     /translation="LALEMGTNYKYLCDAIQESCGLTFNDYLNGFRLDHARNLLYDLK
                     TNPVIEDVLLSSGFTNKSTFYRLFRQKFGLTPTELKTLVKEEMKNSK"
BASE COUNT         1000 a          735 c          717 g         1035 t
ORIGIN      
        1 tgttacagtc cagttactaa tattggtatt tatggtgaaa gagtccttat gttctgtatc
       61 acaaacccaa agggtatcgg ccgaaagttg aatggtcgga taagtttgtg aaagtaccag
      121 gtttttgttt accccattta ccgaaacggt aagtgtatcc gactcatcgg ccagcaggtt
      181 cagttcagca gccgtaaccg ttatcgaatc gttgccatag ccggaataag aatcgagcgt
      241 ataccaactt tccgacgggg atatgctcca tgctgcattc gatttgactg caaacttctt
      301 ggaattgcca ggaatacttt ctacagacag aacagagtct gacaaagtaa gcttgcaggc
      361 tgcttccgaa acatcgtaat tgtcaaccca gttcgacgat gttcccgaaa gggcaaagtt
      421 gaacaattct ccattaatag acggcgaagc tgcctgtgag taaagcacat caatatcctt
      481 gttatcactt tttgaatatc cttgtttgaa gttgtaataa gtaacaatat cgggttcact
      541 gccggtaatg ccgtttcgca ttaactcaac aatttctgac tgggtcaata ccctatccca
      601 taaccctatt tcgtccatac tgcaatacgg agaagtgatc aggttgtcgc ttatcctgtt
      661 gccgaccgtg aaattatcat aggtgctgct aactggcata taaaccgtac cagaaccaac
      721 ttcttcacca tttacataga tcgtacccgt cccatggata ccatacgagt cggtaatccc
      781 caagcttttt atggtgaaag cgatgtgtgt ccacacattt gccgacagta cgttattaat
      841 tgtaaaactc ttgtaataag actccaggtg aagtccattg ccatagcccg acaaataaag
      901 cgctttatag gaatttgtac ccgaaggaaa aagataaacc aggtatttgt tatattgaag
      961 ttcgtcgaac tttacccatg ctgaataagt tatcccattt aaaaagtcag cattggatgc
     1021 agtgatatca tcgaagatga tgtgatcatc gcttccgtcg aacgagaggg cgttatctgt
     1081 ctcattgtac tggaaaaagt gcagtgtatc ggaaataaaa gcacccgtaa aaacaatctc
     1141 tcccgatcgg aaagctgtcc cttcaggaag actttcagcc gtaaagttaa tagttgtatt
     1201 acctgtgcct tcggaactac ttagagtgag ccacgatgca ttgcccgtag ccgtccatgc
     1261 aacaggagtg gttatgccga cagatcctga agtggttggc gaaataaatc gaaaagagtt
     1321 ttgagaaagg gttatcgtgg gtcggagttg tgttacaact attgtatcgt caccactgat
     1381 tgtaccttta acaataacct ctgcactacg atcggcttcg gtttcatttg ttgttatggc
     1441 tgttaaagta agtgttgcat cggttgatcc ggatgttgga cttaccgaaa gccagtcact
     1501 cgactcgact accatccacg tgtcaccatc attaacgata tcgactgttg atgtgctgtt
     1561 ttggccagca tcaagtgtat ccaaacctga tgaaagtgtt aatgataccg gatcttgagt
     1621 tactaccacg gtaaccggct gcgaaacatc tgtactgctt agtgtaatgg ttgctgaacg
     1681 gctagtgttc gacgaattga cagcagttgc tgtaatggta atcgaatcac taaatgatcc
     1741 tgatgtttta tcaagagaaa gccatgaact actttctgta actgtccatt tagtatttga
     1801 aacaatatca attttagctt tgctgttaag cgcccttccc aacgaaatag tatcggtaga
     1861 aggggtaatt gaataaacaa cttgactggt ataaccgctt acccagtttg acgaagtacc
     1921 tgaaagtgta aaattcctca gtgttccgct aatgtttggc gaagaaacct tcgacgtaag
     1981 tgtttgaacc gacgtgtttg taccattggc cgttccctgg ttgaattgat aatacaaagc
     2041 taatccgctt tcagaaccgg taaagccact ttttatacaa ttgtagactt ccgtttggct
     2101 caattccctg ttccaaagac ttacttcatc gagtacaccg ttaaagtaat atttggtcaa
     2161 atttttaatg taatcgtaat aagctcccag cacattgcca gtccggttta tattattggg
     2221 catgctataa gtcggattgg tagccaccaa catgccatcg agatacattt ttacatatcc
     2281 gtttccttca gtaaaagcaa aatgatacca gcgtcctgtc accatctgtg tggatgataa
     2341 tgacacactt gacccgccgg taacaggact ccaagcctga taacccaacg aggtttgacc
     2401 tccgtatgta aactgaaaaa atcggatgcc atcattgtcc ccaatatcaa acataccctc
     2461 agattctgca agtgcattaa attttaccca tagggttaac gacactgcct gcgaaaaatc
     2521 gggattactt gccgctaagg ttcctaactc gacataatcg tcagtcccgt caaaactcag
     2581 ggcattattt tgtgctgccg accattgcgg caaaagcagc gatagcagga tgaacgtgaa
     2641 tagccacatg atcctgtgct taattttcat ttgtagaaat ttcaacatga tttattattt
     2701 tgattttgta atttgttttc agtagttaat acgtttactt tcattgaaat atcctccatt
     2761 aaaggattat ttcgccgaac aatgcttcgg acacttttgc tgtcgaggaa cgaaagcagc
     2821 attttatgcc caagggccag aaacatctca tcaaaccggc ggcaatagag tagtttgatg
     2881 tttctgttat tgccttcgaa atcgttgttt aaaatctgtt caatgttgag ggtggttgag
     2941 atgatccact ctttatgttt gcccgtctgg agaatgtaaa cccagaatgc attatttgaa
     3001 gtttccgttt tcatatactc ttcatttatt tcttcattcg agagcaaatg tattttcaag
     3061 gccaattggg gtagtctcat atttgcaaaa catagtctca tatttgcaaa acactcttaa
     3121 acagataaat aaaaattgga tttaaaaaat caaaagtaca aaaaaaccgg atcagaaaat
     3181 ctgtccggtc tctatatttc ttttcaaaag actacttact attcttcatc tcttctttta
     3241 ccaacgtttt gagttcggtt ggcgtgagcc cgaatttctg acggaaaagc cggtaaaatg
     3301 tactcttatt cgtgaagccc gacgagagca gtacatcttc gattaccggg ttggtcttta
     3361 aatcgtacaa caggttgcgg gcatggtcga ggcggaaacc attcaggtag tcgttaaagg
     3421 taagcccaca ggattcctga atggcatcgc aaaggtactt gtaattggtt cccatttcga
     3481 gggcaag
//
LOCUS       DAOPYB010000267         3478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_36356_length_3478_cov_5.712241, whole genome shotgun sequence.
ACCESSION   DAOPYB010000267 DAOPYB010000000
VERSION     DAOPYB010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36356_length_3478_cov_5.712241"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..1578)
                     /locus_tag="PKH58_12790"
     CDS             complement(391..1578)
                     /locus_tag="PKH58_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNX89949.1"
                     /translation="MFSKEFLKRFHGQEDVDRFMNDLHSKLYEQLLNAEMDEHLGYEK
                     NSTEGNNSGNSRNGSYNKTIQTLHGEAGINVPRDRNGEFEPVIIPKHANKGMSLEKLV
                     ISLYAKGLSTSDIEDELRDIYQINLSSSAISIITNKVTQAALEWQNRPLERQYFVVWM
                     DGIVFKVRDGGKVINKTVYLCVGLDKTGHKEVLGMWVGKAESSSFWMSVLSDIKARGV
                     EDILITCTDNLNGFTDTIRTVFPQAATQICVVHQIRNSCKYVVWKDLKEFTADMKNIY
                     GAINKEAALAALDDFEKKWKSKYMYAVRSWRNNWEDLTTFFEFPIDIRRIIYTTNLIE
                     NLNGKIRKYTKTKLSYPNDDAVKKSVYLAIGEIEKKWTVPIRNWGIVLNQFIAIFEDR
                     ILL"
     gene            complement(1747..2418)
                     /locus_tag="PKH58_12795"
     CDS             complement(1747..2418)
                     /locus_tag="PKH58_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89950.1"
                     /translation="MKLKLVTAALLLTVSTVMAGNWKGPKITEVSLDSLRAQRTAQNM
                     LDEEYLKRLKSLKETVDGNLSEIDKSIEEVKTAQSDVKAQQELLKQQRNTLKAEENNI
                     KAKNKVLDQESKNIVKEEKDLKKNNLLSNEEKQLKTKSLEERASKNGKDLEATKKRSN
                     EIQKEYKDVDKKAADYTTMATDVSNRAAELQALRTKWKLQSDTIKQEISLVKNTIKTN
                     KKLQH"
     gene            2630..>3478
                     /locus_tag="PKH58_12800"
     CDS             2630..>3478
                     /locus_tag="PKH58_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89951.1"
                     /translation="MNYSKFLQNIFFAILVFALSFTQLQAQDYQISANGTQSVMACTT
                     GDVYAWGKNTDGALAIGNTTESIYRPTKVLMPAAAGKIQHVDGSSGAFLLAINCNGEV
                     WIWGDNQFGQCGNSTQTTKVATIPVFLMGGQATATPTAKISGIKAISGGSSTGYAITA
                     DNRLIAWGNGADGRLGNGTSTTSNLPVYVRDKNGKILENVIMVDGGDNTCYCLVDDDG
                     DGLGTVYSFGQVNGSMLGRSTSSINYYALPVEKEDGSVLDNIKVVAAGDVHGTALDAN
                     NYVWIWG"
BASE COUNT          889 a          747 c          626 g         1216 t
ORIGIN      
        1 aatatcatca ccggctactg gtgcaacacc acctacccag tttgagcctg ttgtccagtt
       61 caatcctgta ccggttcccc cattccacgt ttttgttgcc gcagaaatag tcacagaaat
      121 aatcattgcg agtgtaaaaa gaaaaagact tttgcggctc atagtttcaa gatttataat
      181 taacaaacgt ataatgcgct tattgagcac gcactaccac cttatattat acacaaagta
      241 atataattat tttcatatta ttttaaaaaa aatcactttt ctattgtaat ttttttaccc
      301 accaattgta tttctcccgc aaaacaatgc ggaaaacttt gggtaaggca ccgtccatga
      361 ttttgtgtaa atgaaaaaat aaggcctttg tcataataaa atcctatctt caaaaatagc
      421 aataaattgg tttaatacaa taccccagtt tcttataggc acagtccact tcttttctat
      481 ttctccaatg gcaagataaa cagacttctt gacggcatcg tcgttgggat aggaaagttt
      541 ggttttcgtg tatttccgta ttttgccgtt gagattttca ataaggttgg tagtgtagat
      601 aatgcggcgg atatctatag ggaactcaaa gaaggtggtc aggtcctccc agttgttgcg
      661 ccagcttctg accgcataca tgtactttga tttccatttc ttctcaaagt cgtctaatgc
      721 ggccagagca gcctctttat tgattgcgcc atagatgttt ttcatgtcgg cggtaaactc
      781 tttcagatcc ttccatacca cgtatttgca actgtttctt atctggtgca ctacgcatat
      841 ctgtgtcgca gcttgtggaa agactgtgcg tatggtgtcc gtaaaaccgt tcaggttatc
      901 tgtacaggta ataaggatat cttccacgcc acgggccttt atatcgctca gcaccgacat
      961 ccaaaacgag gagctttccg ctttacctac ccacattccc agaacttcct tatgacccgt
     1021 tttgtctaac ccgacgcata ggtataccgt cttgtttatc accttgcctc cgtctctgac
     1081 cttaaaaact attccgtcca tccatactac aaaatactgc ctctctaacg ggcgattctg
     1141 ccattctaac gcagcctggg tgactttgtt cgtgataatt gagatcgcag aacttgaaag
     1201 gtttatctga tatatgtcgc gcagttcgtc ttctatgtcc gaggtgctta aacctttcgc
     1261 gtataacgat attaccaact tttccaatga catgcctttg ttcgcatgtt tcgggattat
     1321 taccggctca aattctccgt tcctgtccct cggtacgttt atccctgcct ctccgtgaag
     1381 tgtctggatt gttttgttgt aactcccgtt ccttgagttg ccgctattgt tcccttccgt
     1441 actgtttttc tcatagccta agtgttcgtc catttctgca tttagcaact gctcatacaa
     1501 ctttgaatgt aggtcgttca taaaacggtc tacgtcctct tgcccgtgaa atctcttcaa
     1561 aaactctttg ctgaacattt cgccaggtac ttcatagaac tcttcaggct ctttcttgtc
     1621 ctttttcttt gtcatttctt ttgtgtacat atctatttgc gttaaagtta tcctttttct
     1681 ttaacctcaa atttcattta cacaaaattc tagatagtcc ctttttttta tttctgtagc
     1741 taaaaattaa tgttgtaatt ttttatttgt tttgattgta ttcttaacca aagaaatttc
     1801 ttgtttaatt gtatccgact gcaatttcca tttggtgcgc aaagcctgaa gttcagctgc
     1861 tcgattcgaa acatctgttg ccatggttgt atagtcagca gcctttttat ctacatcttt
     1921 gtattctttt tgaatttcgt ttgaacgttt tttggtagct tctaaatctt ttccattttt
     1981 tgatgctctt tcttccaaac tcttcgtttt cagttgtttt tcttcgtttg aaagcaaatt
     2041 attcttcttt aaatcttttt cctccttcac tatgttcttg ctttcctgat ctaatacttt
     2101 attttttgcc ttaatattat tctcttctgc ctttaaggtg tttcgttgtt gtttcaacaa
     2161 ttcttgttgc gccttaacat cactttgtgc agtttttacc tcttctatac ttttgtcaat
     2221 ttccgaaaga tttccatcta ctgtttcttt caatgacttt aaacgtttta aatattcctc
     2281 atctaacata ttttgtgctg ttctttgagc acgaagagaa tcaagactta cttcagtaat
     2341 ttttggacct ttccaattac ctgccattac agtgcttact gtcaataaca aagcggccgt
     2401 tactaacttt aatttcatat catttttgtt ttaaaattta actatgcaaa ggtaacaaat
     2461 tcatcgggtt actatcccaa ttttggagaa acgcgctttt cattttcgtt tgcaactata
     2521 attttactca attgactagc ttccaattat ttttacttga aatcctttgg tatttccttt
     2581 actttagtgt aattttaaca ctaagatttt tttactttaa acgataatta tgaattattc
     2641 aaaattccta cagaatatat tttttgcaat acttgttttt gccttgtcat tcacgcaact
     2701 acaagcgcaa gactaccaga tttcggcaaa tggtacgcaa tcggtcatgg cgtgtaccac
     2761 cggcgatgta tatgcgtggg gaaaaaacac cgatggagcc cttgccatag gcaacacaac
     2821 cgaaagcatt tatcgcccta ccaaagtgtt gatgccagca gctgccggaa aaatccaaca
     2881 cgttgacggt agttcgggag catttctttt ggctatcaat tgtaatggcg aagtatggat
     2941 ttggggtgat aaccaatttg gacaatgcgg aaacagcaca caaaccacga aagtcgccac
     3001 catccctgtg tttcttatgg gagggcaagc aacagctact cctaccgcaa aaatatcggg
     3061 catcaaagct atatcgggcg gaagctctac aggctatgcc attaccgccg acaatcgttt
     3121 gatagcatgg ggtaacggtg ctgatggacg tttaggaaat ggaacctcta ctacaagtaa
     3181 tttgccggtg tatgtgcgtg acaaaaatgg caaaatcctt gaaaatgtca tcatggttga
     3241 tggtggtgac aatacttgct actgcttggt tgacgacgat ggcgatgggc tcggcaccgt
     3301 gtattctttt ggacaagtaa atgggagtat gctgggacgg agcaccagtt ctattaatta
     3361 ctatgccttg ccggttgaga aagaagatgg atctgttctt gataacataa aagttgtagc
     3421 agccggcgac gttcatggaa cggcattgga cgcaaataat tacgtttgga tttgggga
//
LOCUS       DAOPYB010000268         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_36793_length_3451_cov_9.552120, whole genome shotgun sequence.
ACCESSION   DAOPYB010000268 DAOPYB010000000
VERSION     DAOPYB010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36793_length_3451_cov_9.552120"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1215
                     /gene="mltG"
                     /locus_tag="PKH58_12805"
     CDS             193..1215
                     /gene="mltG"
                     /locus_tag="PKH58_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNX89952.1"
                     /translation="MSGNKNILKSLLLSGGVIVLFVVYVFFMPNISPKGEKVTFLFIA
                     DNTAYSEVIDQLEEKAEINSVFSFNLAAKILNLKEIKSGRYKIAKGTANFRLIRNLKN
                     GHQTPVQLKLNNIRTKEQLAGKLAAQLMPDSAKFLILLNDTTFLAGYNLNPNNAIAIF
                     LPNTYEIYWNTNEKKLFERMSREYLKFWTDTRKQLASAIPLSPEEVITLASIVDEETN
                     SKSEKSIVAGLYINRLKKNMPLQADPTIKFAVGDFSIKRVMYGHLQTNSPYNTYKFTG
                     LPPGPIRIPNLETIDAVLNYYKSDYIYMCAKETLNGEHNFAADWAQHKINAAKYQKAL
                     NERGIK"
     gene            complement(1285..2556)
                     /gene="serS"
                     /locus_tag="PKH58_12810"
     CDS             complement(1285..2556)
                     /gene="serS"
                     /locus_tag="PKH58_12810"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNX89953.1"
                     /translation="MLTLKYITENATEVIERLAKKHFDGNQIINQIIDLDERRKITQT
                     AADNFSAELNSLSKEIGQLFKTGKQTEANEAKEKTIVLKENISKLNEQRDEIEQKLQE
                     LLVLVPNLPHDSVPEGKHAEDNKIERTGGIIPELSPDALPHWDLAKKYNLIDFELGVK
                     ISGAGFPVYKGKGARLQRALINFFLDNARDAGYLEVQPPYVVNAASGYGTGQLPDKEG
                     QMYHCGLDDLYLIPTAEVPVTNIYRDVILDEKELPIKNTAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVRIDTPEHSYESLTEMVNYVQSLVEKLELPWRILRLCGGDMSFTSA
                     LTFDFEVYSTAQQRWLEVSSVSNFESYQANRLKCRYRTADKKTQLCHTLNGSALALPR
                     IVAALLENNQTPEGIRIPKALVPYTGFEIID"
     gene            complement(2734..3000)
                     /gene="rpmA"
                     /locus_tag="PKH58_12815"
     CDS             complement(2734..3000)
                     /gene="rpmA"
                     /locus_tag="PKH58_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNX89954.1"
                     /translation="MAHKKGQGSSHNGRESESKRLGVKIYGGQFAKAGNIIVRQRGTV
                     HNPGLNIGMGKDHTLFALIDGTVEFRKKRDNKSYVSVRPIEDNN"
     gene            complement(3021..3335)
                     /gene="rplU"
                     /locus_tag="PKH58_12820"
     CDS             complement(3021..3335)
                     /gene="rplU"
                     /locus_tag="PKH58_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNX89955.1"
                     /translation="MYAIVEILGQQFKAEQGKRLFIHRMAEAERGATVEFDKVLLVDN
                     NGAVTVGAPVVEGAKIVCEVISHVRGEKVIVFHKKRRKGYRKRNGHRQDFTELTIKEI
                     IA"
BASE COUNT          972 a          702 c          655 g         1122 t
ORIGIN      
        1 gttacccgta cgtcagttta gcaaactggt ggtttcagcc actcacccac acttccatgg
       61 ctgcgtttct gattttgcga gtgcaaatat acttaaactt ttattatcaa caaaaccttt
      121 cactacaaaa ttattcaaaa acaatttaat aattttgccc attataatat ataaataacc
      181 caaaatatgc atatgtctgg aaataagaat attctcaaat ctttactcct ctcaggaggt
      241 gtaattgtac tgttcgttgt atatgtcttc tttatgccca atatcagtcc aaagggtgaa
      301 aaagtaactt tcctgttcat tgctgataat acagcgtata gcgaagtgat tgatcagttg
      361 gaagaaaaag cagaaattaa cagtgtattt agttttaatc ttgcagcaaa aattcttaat
      421 ctcaaagaaa tcaaatccgg acgctataaa atagctaagg gaactgcaaa tttcaggtta
      481 atccgtaatc tgaaaaacgg acatcaaact cctgttcaat tgaaattgaa taatatccgt
      541 accaaagaac aacttgccgg aaaactggct gctcagttga tgccagattc ggcaaagttt
      601 ctgattttac tcaacgatac tactttcctg gcgggttaca acttgaatcc aaacaacgcc
      661 atcgcaattt tcctcccgaa tacctatgag atttactgga atacgaatga aaagaagctt
      721 tttgaacgta tgagcaggga ataccttaaa ttctggacgg ataccagaaa acaattggct
      781 tcagccattc ctttgtcgcc cgaagaggtg attactcttg cctctattgt tgatgaggaa
      841 acaaacagta aaagcgaaaa atcaatcgtg gcagggcttt atattaaccg attgaagaaa
      901 aatatgccat tgcaggcaga tcccactatc aaatttgcgg taggcgattt ttcaataaaa
      961 agggttatgt atgggcactt gcagaccaac tctccatata atacatataa gtttaccggt
     1021 ctgcctccgg gacctatcag aatacccaat ctggagacca ttgatgccgt gctgaattat
     1081 tataagagcg attacattta tatgtgcgcc aaggaaacac ttaatggcga acataatttt
     1141 gcggcagact gggcgcagca taagattaat gctgccaaat atcaaaaagc attgaatgag
     1201 cggggtatta aatagttata aactgttaaa taaaaaaaca ggataatcat tatgttatcc
     1261 tgttttttaa tcaaaatgtt ttttttaatc tattatttca aagccggtgt aaggaaccag
     1321 cgcttttgga attctgatac cttcaggcgt ctgattattc tcaagtaagg cagccacaat
     1381 gcgtggcagc gctaatgcac ttccgtttaa tgtgtgacat aattgcgtct tcttatctgc
     1441 agtccggtaa cgacatttca gtcggttggc ctgatagctt tcaaaattcg aaacagaact
     1501 aacttccagc cagcgctgtt gagcagttga atacacttcg aaatcaaaag taagggcaga
     1561 agtgaagctc atatcaccgc cgcaaagccg aagaattcgc catggaagtt ccagcttttc
     1621 aaccagtgac tgaacataat tcaccatttc ggtcagcgat tcgtatgaat gttcaggggt
     1681 gtcaatacgt acgatttcta ctttatcaaa ctgatgcaga cggttcagtc cgcgtacatc
     1741 ttttccgtac gaaccggctt ccctgcggaa acatgccgag tacgcagtat ttttaatcgg
     1801 taattctttt tcatctaaaa ttacatcgcg gtagatattg gttacaggta cctcagcggt
     1861 cggaattaaa tataaatcat ccagtccgca atgatacatc tgaccttcct tgtcgggtaa
     1921 ttgtccggtg ccataacccg aagcggcatt cactacatag ggtggttgta cttccagata
     1981 accggcatcg cgcgcgttgt ccaggaaaaa gttaatgagt gcccgttgaa gccgtgcgcc
     2041 tttaccttta tagacaggga agcccgctcc gctaattttt acaccaagtt caaaatcaat
     2101 gagattgtac ttttttgcca aatcccagtg ggggagagcg tcggggctta gttcgggaat
     2161 tattccacct gttcgttcaa tcttattgtc ttcggcatgt ttgccctcgg gtacgctgtc
     2221 gtgaggcaga tttggaacca gcaccagcag ttcctggagt ttctgttcta tttcatcgcg
     2281 ttgttcgttg agtttgctga tgttttcctt cagtacaatg gttttttcct ttgcctcatt
     2341 ggcttcggtt tgtttgccgg ttttgaaaag ttgtccaatt tctttcgata aactgttcag
     2401 ttcagctgag aaattatcgg ctgctgtttg agttatttta cgtctttcgt ccagatcaat
     2461 aatctgattg ataatctggt taccgtcaaa atgctttttt gccagtcgtt caatcacttc
     2521 tgtggcattt tctgttatgt atttcagtgt aagcatatat ttgttgttat tataatttta
     2581 ctgatattaa ttttacgtgg acaggatttt tctatttctg tttattttca gaattgcaaa
     2641 gttacaaaaa atgctctgaa tagttctttt aaacaaaaaa atctctcaca aaatacattg
     2701 tgagagattt ttatatccgg taagaacttt gtcttaatta ttatcttcaa ttggacgaac
     2761 agaaacataa gatttgttgt ctcttttctt tctgaattcc acagtaccgt cgatcaatgc
     2821 aaacaaagta tgatcttttc ccataccgat gttcagacct gggttatgaa cggtaccacg
     2881 ttggcgaacg atgatgttgc ctgcttttgc aaattgtcca ccgtaaatct ttactccaag
     2941 tcgtttgctt tccgattcac gaccgttatg tgagctacct tgaccttttt tatgtgccat
     3001 tttcttctaa atttaatcgg ttaagctata atttctttaa ttgtcaattc ggtgaaatcc
     3061 tgacggtgac cattgcgctt gcggtaacct ttgcggcgtt ttttgtggaa aacaataact
     3121 ttttcacctc ttacatgcga aattacttcg caaactattt ttgcgccttc taccacaggt
     3181 gctcctacgg ttactgcgcc attgttgtca accaacaata ctttgtcaaa ttcaactgtt
     3241 gcacctcttt cggcttctgc catgcggtgg atgaacagtc ttttaccttg ttcagctttg
     3301 aactgctgac ccaggatttc tacaattgcg tacatctata ttgtatttta taattacatc
     3361 gggaaggtga gttgtcatta ttttgattga cattgcttct ttttaaacct tttccgaatt
     3421 gagggtgcaa atatacgtca ttttctccaa a
//
LOCUS       DAOPYB010000269         3431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_37117_length_3431_cov_7.493780, whole genome shotgun sequence.
ACCESSION   DAOPYB010000269 DAOPYB010000000
VERSION     DAOPYB010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37117_length_3431_cov_7.493780"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1164)
                     /locus_tag="PKH58_12825"
     CDS             complement(232..1164)
                     /locus_tag="PKH58_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNX89956.1"
                     /translation="MKTTFIKSALAAFTIVAIVSFTSCAKEDDTTPAPDSKNIVQVAK
                     SDNQFSILAAAIEKAGLTSTLESAGPFTVFAPTNDAFNALFSQLGVSGINDLSAETLK
                     PILLNHVLAGSVKSSAITTGYFATANTYGPGQQAVKVYVKKESGVMVDGSNVTTADVM
                     ASNGVIHVIDKVILPSTVVGHALNNPNFSILVQAVVKAGLVDALNGSGPFTVFAPTND
                     AFNALFATLGVSGIDALTAEQLTPILLYHVVPGNVTASQVATGNVPTLKDGSNISVVA
                     GSSGVKLNSSSNVIATDVQGTNGVIHAIDAVLLP"
     gene            1690..2097
                     /locus_tag="PKH58_12830"
     CDS             1690..2097
                     /locus_tag="PKH58_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89957.1"
                     /translation="MMSVKSTIALSACIILLLSTSSCEKEPGEGGNSSIYGKVIVRDY
                     NAEFTYLKEVYPGRDVDVFIIYGDDKSVSDRVRTSYDGTYEFKYLRKGEYKIFAYSKD
                     STLMTNSVVPVIKDVTIDDNKTEVEVPDLIIYN"
     gene            2103..2666
                     /locus_tag="PKH58_12835"
     CDS             2103..2666
                     /locus_tag="PKH58_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89958.1"
                     /translation="MKYLIILFIFCLSFSQSYGQSKKELSSAKVKSSTEWSTKKEDGK
                     TTSYKSLYEEYDKSGNTTLSIEYDEAGNQIHKEVVVYDKFKRVTSETVFDASKEKTVR
                     KSYIYDAFNNVKEEMEYNGDKLIKKTVYTYNADGNKTSESTFDGLGELMKKATFTYDA
                     KKLKSGRTTVNPAKGTETVKKWAYTYF"
     assembly_gap    2702..2711
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2712..>3431
                     /locus_tag="PKH58_12840"
     CDS             <2712..>3431
                     /locus_tag="PKH58_12840"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNX89959.1"
                     /translation="SVFEPISLEQMDKVKLLNRVDTKFVVNQNRLPELLEGLNEQYYV
                     LDIDGVRIYPYHTTYFDTEDLLAYRNHHNGKLNRYKVRLRTYMISGQNFFEIKFKNNK
                     RRTIKSRVTVHSGTPVITGKAEELLLKKTPFIASDLQPRLSVNFHRTTLVSKKLNERI
                     TIDTGISFTRDSQQKNYNGLLIVEVKQDKSQESPILKRLHHMHVNPARLSKYCLGVLS
                     MYEGVKYNLFKETLLNINRIL"
BASE COUNT         1108 a          660 c          738 g          915 t
ORIGIN      
        1 aaaggcgaag cccgccgaat gcttaaagac ggaggcgttg ccattaacaa agccaaagtg
       61 gctgaggacc ttatcgtgac tgatgaattg ttgcttaacg acagttatat cttagtgcag
      121 aaagggaaaa agaattatta cctggttaaa gcagtctgag actaaccttc atcataaaaa
      181 caaaaaagcc cgccaaaccg gcaggctttt tttctgtcag attttttcag tttacgggag
      241 cagtacagcg tcaattgcat gtattacacc attagttccc tgcacatcag tagcgataac
      301 attggatgag ctgttgagct taacacccga tgaaccggct acaacactaa tgttactgcc
      361 atcttttaaa gtaggaacat taccggttgc gacctgagaa gcagttacat tgcccggaac
      421 aacatggtaa agcaggattg gagtaagctg ttctgctgtt aatgcatcta tacctgatac
      481 acctaatgtt gcaaacagtg cattgaaggc atcattggtg ggagcaaaca cagtgaaagg
      541 tccggaaccg ttgagggcat caacaaggcc tgcctttaca acagcctgaa ccagaatgct
      601 gaaattgggg ttgttgaggg catgtccgac cacagtggat ggaagaatca ctttatcaat
      661 cacatggata acaccattgg aggccattac atcggcagta gtcacattgc tgccatctac
      721 cataactcct gattctttct taacatacac tttaactgct tgctgaccag gaccgtaagt
      781 atttgctgta gcaaaatatc ctgtagtaat agcagatgat tttactgatc cggccagtac
      841 atgattcagc aggattggtt tcagggtttc ggcactcagg tcgttgatac cactgacacc
      901 caactgactg aaaagagcat taaatgcatc gtttgtcgga gcaaacacag tgaaaggccc
      961 tgcactttcg agggtagaag taagtccggc tttctcaatg gcagctgcca gaattgaaaa
     1021 ctggttgtcg gatttggcta cctgtacaat attcttactg tcaggtgcag gggttgtgtc
     1081 gtcttccttt gcgcaggaag tgaatgatac tatggcaaca attgtaaaag ctgccagcgc
     1141 tgatttgatg aacgttgttt tcatggctat ttgattttgt ttattattta cttgattacg
     1201 ttaaacacga atataaaacc gaatagcatc agaaatgttt aatgatggct ataaaaaaat
     1261 taaacaaaaa tttatttcca gatagaatgc gctataaatc agtcgataac aatttggttt
     1321 attttgaaaa aaaattcaac atcgaaatca tctgtagccg caacgaagaa gcaactttta
     1381 ataatgtata aaattctgct atcgtcgaaa tcgcatcctg tgaccataaa aactgcttgt
     1441 ttatctattt agtaaggcag attttctaag tgtatattat gtttgccgcg taatgtaagg
     1501 cagttcagtc tgttgtatta tttcagtctt gacaatgagt ccgatagacc ggggcagagg
     1561 aagtgtcgtt aaccattgtt tttagtagca gaagtcagac taaaaccggg aaggatggca
     1621 tttaatttag tactattgca cgcccaatca gaacacatta caatcctctg aaaaataact
     1681 tcttttacta tgatgtcagt aaaatcaaca atagcgcttt ctgcatgcat catattgctg
     1741 cttagtactt cctcttgtga gaaggagcct ggcgaaggag gaaactcctc catttacggg
     1801 aaagtaattg taagggatta caatgctgaa ttcacctatt taaaggaagt ttaccccggc
     1861 agagatgtgg acgttttcat tatttatggt gacgataaaa gtgtgagcga cagggtgcga
     1921 acttcttacg acggaactta cgagtttaag tatctccgca aaggagagta taaaatattt
     1981 gcctactcaa aagattccac cctgatgacc aattcggttg tacctgttat taaggacgta
     2041 acaattgacg ataacaaaac cgaggttgaa gtacccgatc tcatcattta taattaatgc
     2101 aaatgaaata cctgatcata ttattcattt tctgcctgtc attcagccag tcatacggac
     2161 agtcgaaaaa ggaactttcg tcggccaaag taaaatcgag tacggagtgg tcaaccaaaa
     2221 aggaagatgg taaaacgacg agttacaagt cgttgtacga ggaatacgat aaatccggca
     2281 acactacact cagcattgag tatgatgagg cagggaatca aatccacaag gaagtggtgg
     2341 tttacgataa gtttaaacgc gttacttcag aaacagtttt cgatgcgtca aaggaaaaaa
     2401 ccgtgagaaa atcttacatc tacgatgcgt tcaacaacgt gaaagaagag atggaatata
     2461 atggtgataa gctgattaag aagacagttt acacatacaa tgccgatggc aacaaaactt
     2521 cagaaagcac ttttgacggg ctgggtgaat tgatgaaaaa agccacattt acctacgatg
     2581 ctaaaaagct aaagtcaggc agaactactg tgaatcctgc caaaggaacc gaaacagtta
     2641 aaaaatgggc ttacacatat ttctgatcct gttttgttta tgagtaccga aaatggcatt
     2701 tnnnnnnnnn ntgtcagtat ttgagcccat tagcctcgag cagatggata aagtgaagct
     2761 gctcaacagg gtcgatacca aatttgtggt caatcagaac cggcttcccg aactgttgga
     2821 aggcctcaat gagcagtatt atgtgcttga cattgacggt gtaagaatat atccttatca
     2881 cacgacctat tttgataccg aggatttgct ggcataccgg aaccatcata acgggaagct
     2941 caacaggtac aaagtcaggc tgaggactta tatgatttcg ggtcagaatt tttttgagat
     3001 caaattcaag aacaataaaa gaagaacaat aaaatccaga gttactgtac actccggaac
     3061 cccggtaatt acaggcaagg ccgaggagtt gctcttgaaa aaaacacctt ttatagccag
     3121 cgaccttcag cccaggttgt cagtgaattt ccaccggaca actctggtaa gtaaaaagct
     3181 gaatgaaagg attacaatcg acacaggaat ttcatttaca agagatagtc agcagaagaa
     3241 ctacaacgga ttgctgatag ttgaagtaaa acaggataag tcacaggagt cacccatcct
     3301 taagaggctg caccatatgc acgtgaatcc agcacggctc agcaaatatt gccttggagt
     3361 gctttcgatg tacgaaggcg taaaatacaa tttgtttaaa gaaactttac ttaacattaa
     3421 caggatactc t
//
LOCUS       DAOPYB010000270         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_37381_length_3415_cov_6.410714, whole genome shotgun sequence.
ACCESSION   DAOPYB010000270 DAOPYB010000000
VERSION     DAOPYB010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37381_length_3415_cov_6.410714"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..>1238)
                     /locus_tag="PKH58_12845"
     CDS             complement(31..>1238)
                     /locus_tag="PKH58_12845"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNX89960.1"
                     /translation="VSPQENIILLSAKIHPSEAELQALNAQIELVHDWELMTRNLTER
                     GMAPLFYTKLPKLSNRTLIPTVSYGHLKQVYYRTLTRGMMLYEVFRRVADALTLNGIR
                     VVALKGVHLAEWLYQDIGLRQFSDIDLLVRKEDAEKCLKILRDMGFVQSGDEVSQLIM
                     EKSGIIHYPAMVMNDISIEIHINLNRPGKAEHLDARKFIHCAEPLVINHSRVHVLELY
                     DLLIFLCVHLEKHFQSGELQFTCFSDLTNLIDIRYDEIDWQKLEIRSKLSRVEKTVYK
                     NLYLVHKYFHARMPEYVADQYSKYLQVHDEELFLDYLNGFRYYSLGFSTHIENIGRTM
                     GIWQKLRYFALVAYPPRKFMVQSFRIKHPELFWLYYPARHWKGLRGMWKMVSFRAKTR
                     KMMNDENKD"
     assembly_gap    1239..1338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1592..2401)
                     /locus_tag="PKH58_12850"
     CDS             complement(1592..2401)
                     /locus_tag="PKH58_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNX89961.1"
                     /translation="MIQLNQIRQYFPQQIRENSIFDKYILKEYVQLMILDFLSSTRYI
                     RKICFIGGTNLRLVSGIDRFSEDLDFDCKGLAKEEFDAMTSEIVQFLKRSGFRVETRD
                     TPNSKLTAFRRNIYFPELLFELNLSGHKEERFLIKIESQDQGIEYTNHLVDVRGCGFF
                     FPFPVPDNRVLCSMKIAAMLSRAKGRDFYDLMFLLTRTQPDYEFLSARCGINNSHELK
                     EAIFRLLDTVDLSKKQRDFEHLLFNKENSTKILRFREFIEPGLLEPRREDL"
     gene            complement(2398..3015)
                     /locus_tag="PKH58_12855"
     CDS             complement(2398..3015)
                     /locus_tag="PKH58_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89962.1"
                     /translation="MNFLQFKERMFPLACFNINQVLSFQPDFDRNNISRWIKKGYIIK
                     LRQSYYTFPEYRSKPGYSLYFANRMYNPSYISLHSALSFYGLIPEAVVQITSVTTLKT
                     IFFANDLGEYSYKSVKENMMFGYELKPMSDDRTLKIATPEKALVDLLYLYPFYDNDRE
                     LEELRLDEEFLQQDFGVERMRQYASEIGSKALDKRIIRMCNVYGI"
BASE COUNT          943 a          732 c          623 g         1017 t
ORIGIN      
        1 tactctccca gtctcaaagt cctggttcta tcaatctttg ttctcatcat tcatcatttt
       61 tctcgtcttt gctctaaaag aaaccatttt ccacatcccc cgtaatcctt tccagtgcct
      121 cgccggatag tacagccaga agagttccgg gtgttttatg cggaaacttt gaaccataaa
      181 tttccggggt gggtatgcca cgagtgcaaa gtaacggagt ttttgccaga tacccattgt
      241 cctgccgata ttttcaatgt gggtcgagaa tcccaaagaa taatatctga aaccattcag
      301 atagtccagg aataattctt catcgtgtac ctgcaggtat ttggaatatt gatcggcaac
      361 atactctggc atccgtgcat ggaaatattt atgtaccagg taaaggtttt tatataccgt
      421 tttttcaacc cggctcagct tgctgcgtat ttcaagtttc tgccagtcaa tttcatcata
      481 cctgatatca atcaggttgg tgaggtcact gaaacaggta aactgtagtt ctccgctctg
      541 gaaatgtttt tcgaggtgaa cacaaaggaa aatcagcaaa tcgtaaagtt cgagcacatg
      601 aacccgcgag tgattgatga ccagtggttc ggcgcagtgt ataaactttc tggcatcgag
      661 atgttctgct ttgccgggtc ggttcagatt gatgtgtatt tctatcgaaa tatcattcat
      721 aaccatagcc ggataatgaa ttatacccga cttttccatt ataagttgtg aaacctcatc
      781 acctgactgc acaaagccca tatcacgcaa tattttcagg catttctccg cgtcctcttt
      841 tcttaccagt aaatcaatat cgctaaactg ccgcaaaccg atatcctgat acagccactc
      901 ggccagatga actcctttca gtgccaccac ccggatacca ttcagggtta aggcatccgc
      961 tacacgccgg aatacctcat acagcatcat tcccctggtc agtgtcctgt aatatacctg
     1021 tttcaaatgt ccatagctga cggtcgggat tagtgtacgg ttcgacagtt ttggcagttt
     1081 cgtataaaat agcggcgcca ttccccgttc tgtcagattt cgtgtcatca attcccaatc
     1141 atgtaccaac tcgatttgtg cattcaacgc ctgcaattcc gcctctgatg gatgaatttt
     1201 ggccgagagc aatatgatat tttcctgagg tgacacgtnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnag taatcagtaa tcagtaatca gtaatcagtg atttgttaac
     1381 cagttacaag ccaccagtta caagttaatt cgtgtaattc gtctcaattc gtatagttcg
     1441 taaaaaaaaa cagttcaaca attccacgat tcaacgatta aacagctaaa cagttaacca
     1501 gtcaccagtc tcatactctc ccggtctcaa agtctttgtt cctggctctt ggctcctggt
     1561 tctaaaagtc tttgctctca taattcataa ttcataaatc ttcacgtctt ggctctaaca
     1621 gtcctggttc gatgaattct ctgaaacgaa gtattttcgt gctgttctcc ttattgaaaa
     1681 gcagatgttc aaagtcgcgt tgtttcttgc ttaagtcaac cgtatcaaga agacgaaaaa
     1741 ttgcttcctt tagttcgtgc gaattattta taccacaacg tgccgaaagg aattcatagt
     1801 caggttgtgt ccgcgtcagc agaaacatca gatcgtagaa atcgcgacct tttgcccgcg
     1861 aaagcattgc tgcaattttc atactgcaaa gcacccgatt gtcaggtacc ggaaagggaa
     1921 aaaagaaacc acagcctctg acatccacca gatggtttgt atattcaatt ccctgatcct
     1981 gactttcaat cttaatcaga aaacgctctt ctttgtgacc gctcaaattt aattcgaaca
     2041 gcagttcggg aaaatagata ttacgacgaa acgctgtaag tttagaattg ggagtatcac
     2101 gagtctcaac acgaaaaccg gatcgtttca gaaattgcac aatttcactg gtcattgcat
     2161 cgaattcttc ttttgcaaga cccttacaat cgaaatccag atcttccgaa aagcggtcga
     2221 tgccgcttac cagcctaagg tttgtacctc cgataaaaca tatcttccgg atataccgtg
     2281 ttgaagacaa aaaatcgagt atcatcaatt gtacatactc cttcagtata tacttgtcga
     2341 aaattgaatt ttcacgtatt tgttgaggaa aatactgcct tatctgattc aactgtatca
     2401 tattccgtaa acgttacaca ttcggataat ccgtttatca agtgctttcg agccgatttc
     2461 ggatgcatat tgccgcatcc gttccactcc gaaatcctgt tgtagaaatt cttcatccaa
     2521 acgcagttct tcaagttccc ggtcgttatc gtaaaacgga tacagataga gcaaatctac
     2581 cagagctttt tcgggagtag ctatttttag tgtccggtca tcactcatcg gtttcagttc
     2641 gtaaccaaac atcatatttt ctttaaccga tttataggag tattctccca gatcgttagc
     2701 aaagaaaatc gttttcagtg tagtcacact cgttatctgc actactgctt cgggtattaa
     2761 tccataaaac gataatgccg aatgaagact tatgtatgat ggattgtaca tcctgttggc
     2821 aaaatacaag gaatagccgg gcttgctcct gtactccgga aatgtgtaat agctttgcct
     2881 caatttaatg atatatccct tttttatcca gcggctgatg ttgttgcgat caaaatcggg
     2941 ctgaaaactc aacacctgat ttatattgaa acaggctaac gggaacatcc gctctttgaa
     3001 ttgtaagaaa ttcatttata ctatttttgt ttctgttttt ccgcaaagat aatgaatttc
     3061 ggaaacatat cgcatatttt aatatggaat gaagaatgta gagtgtgtaa tgtgtaatgt
     3121 gggttgctga gctgccagac tgagcatgtc gaagtcctgt cgaagtatga atcgttgaat
     3181 cgttgaattg ttagtgagtg attagtgaaa agttcatcaa gtaagatata gtaatcagta
     3241 atcagtgatt tgttaaccag tcacaagtca ccagttacaa gttaattcgt gtaattcgtc
     3301 tcaattcgta tagttcgtat aaaaaaacaa ttaaacaatt aaacgcttca acgattcaac
     3361 gattaaccag tcaccagtcc catactctcc ccgtctcaaa gtcctgattg ctgat
//
LOCUS       DAOPYB010000271         3382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_37876_length_3382_cov_7.308987, whole genome shotgun sequence.
ACCESSION   DAOPYB010000271 DAOPYB010000000
VERSION     DAOPYB010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37876_length_3382_cov_7.308987"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKH58_12860"
     CDS             <1..462
                     /locus_tag="PKH58_12860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX89963.1"
                     /translation="LHFQYSLLKNCRKNKGNLFGTIRNKFPVIHLGLLGMGTSSLLCG
                     LLPAEMFWFWIFAALCTLLGASSNLYNIPYIAYMQETIPPERMGRAFSLIGSLSSATM
                     PLGLLIAGPITENRGVSLWFFVSGIALLILTLISALFVLPRKADKVGKSNS"
     gene            687..1631
                     /locus_tag="PKH58_12865"
     CDS             687..1631
                     /locus_tag="PKH58_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNX89964.1"
                     /translation="MEQMLYNEYGIQVQSLQQIPAGWSASAWKVHSECGDYFLKVYDK
                     HKPSTRNWIARIDSYMPVVLWLHENTKLQKKMTAPILTRDRTYKKEDSSFLYMVFPFI
                     EGSTICDEKLKPEQIREVAQIISELHFHGAEIPASTNSLIETFDVSFCTTLSNRLGNI
                     HASASLQEVLKPYMTLLAQATESLQSMGLLLQNSKMRYVMCHTDLHGWNLIQSKNLIL
                     IDWEGLKLAPVEADLFSFTGTFFFDYAWEDFMSIYQTTHKDYQINVEAMRFYRLRRRL
                     EDIHEFVESILFDKLTQDDMNQSLRYLKQECELLHVMC"
     gene            1723..2217
                     /locus_tag="PKH58_12870"
                     /pseudo
     CDS             1723..2217
                     /locus_tag="PKH58_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2224..2721
                     /locus_tag="PKH58_12875"
     CDS             2224..2721
                     /locus_tag="PKH58_12875"
                     /inference="COORDINATES: protein motif:HMM:NF019544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX89965.1"
                     /translation="MTGVMASGKSTVAQLLALKMEKGVHLRGDIFRKMIVAGRADMSV
                     QPSEEAIRQLHLRYRLAAETAKTYYDSGFSVVLQDNYYGEELPRMLKMLENYPVHVTV
                     LCPDVETVKRREKMRGKTGYTGFSLEALHADFMRKTPRLGFWLDNSELTPEQSARDIL
                     LHFGE"
     gene            2788..3261
                     /locus_tag="PKH58_12880"
     CDS             2788..3261
                     /locus_tag="PKH58_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNX89966.1"
                     /translation="MNNILIVYYSLFHNTANLALEIAVQTGGTLRELIPDTNYSFEYN
                     TAAKEVRNQISRGFCPKLISGNESIDDYQAIFIGSPNWFKTLAPPVMSFLRLNDFTGK
                     TIIPFCTHGGGGFGQIANDIAKECPKSKILPGIAVTGTVTSEEVTNWLKMIEYEL"
     gene            3288..>3382
                     /locus_tag="PKH58_12885"
     CDS             3288..>3382
                     /locus_tag="PKH58_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047211550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNX89967.1"
                     /translation="MQYGPDPNAIYPNEKIKSVCYIKNIITRPNI"
BASE COUNT         1088 a          615 c          717 g          962 t
ORIGIN      
        1 ttgcattttc agtatagtct attaaaaaac tgtcggaaaa ataagggcaa cttattcggt
       61 accatcagaa ataaatttcc agttattcat ctgggacttt taggtatggg aacatcctcg
      121 ttgctctgcg gtctattgcc tgcagagatg ttctggtttt ggatatttgc agcattatgc
      181 acactacttg gggcaagcag caacttgtat aatatcccct atatcgccta tatgcaggaa
      241 accatcccgc ctgaacgaat ggggcgtgcc ttttccttaa ttggaagcct cagctcagct
      301 actatgcctt tgggattgct catagcaggc ccaattaccg aaaatcgcgg agtatcgttg
      361 tggttttttg tatccggaat cgcactcctc atacttacgc tgataagtgc cctgttcgtt
      421 cttccacgaa aggcggataa ggtagggaaa agtaatagtt gattgttgta acaataaatt
      481 ggaatataac gttaggataa aacactttgc agtatagtat ggaaatggga attttttcaa
      541 caacaaaaag ttatttggga aaagaaattt tatgaataac aatgaaacaa caagtaaaga
      601 aaacctattt ggaacgggta aacttattag ggataaatag aaacttatca tacaattaaa
      661 acgaggtatt tacaatgtat aacagtatgg aacaaatgct ttataatgaa tatggaatac
      721 aagtgcagtc attacagcag attcctgcgg gctggtcagc atctgcatgg aaagttcatt
      781 ctgagtgtgg cgattatttt ttgaaagttt atgacaagca taagccctcc acaagaaact
      841 ggattgcgcg aatcgacagc tatatgccgg tcgttctgtg gttacatgag aacacaaaac
      901 ttcaaaagaa aatgaccgca ccgattctaa ccagagatag aacttataaa aaggaagatt
      961 catctttttt atacatggta tttcccttta tagaaggctc aaccatatgc gatgaaaaat
     1021 tgaagccgga gcaaatacgg gaagttgcgc aaataatttc cgaactgcat tttcatggtg
     1081 ctgaaatacc tgcatcgaca aacagcttaa ttgaaacctt tgatgtttca ttttgtacga
     1141 ctttgtccaa tcggcttggg aatatacatg cttccgcttc cttacaagag gtactaaaac
     1201 cctatatgac attacttgcc caagcgacag aatcgcttca aagcatgggt ctcttactgc
     1261 aaaattctaa aatgcgttat gtcatgtgcc atacagatct tcacggctgg aatctgatac
     1321 aatctaaaaa ccttatcctt attgattggg agggattgaa gcttgcacct gttgaagccg
     1381 acctcttttc atttaccgga acattctttt ttgattacgc atgggaggat ttcatgtcca
     1441 tttaccaaac cacacacaag gactatcaga ttaatgtcga ggcgatgcgt ttttacaggc
     1501 tgcggcgcag attggaagat atccatgagt ttgtcgaaag catcctgttt gacaagctca
     1561 cacaggatga tatgaaccaa tcacttcgtt atctcaaaca agaatgtgaa ttactgcatg
     1621 tcatgtgttg aaaagaacag cttgccttac aaatgcctat ttatatgtag gttttgtatg
     1681 cctaaaatta acaataaaaa aacctttggg aaaggagatt ttatgaataa caatgaaaca
     1741 acaagtaaag aaaacctatt tgatgtttta aatctgttag aagatttaaa tataaaatat
     1801 tggattgatg gtggttgggg tgttgatatc ctgaccgaaa agcaaaatag ggatcataga
     1861 gatatagacg ttgatttcga tggggaatct gaggaaacct tattggctgc actaaaagat
     1921 aagggatata aaatcacgac tgattggagt cctgcacgca ttgagctgca tcacccggaa
     1981 cttggctata tagatataca ccctttaata atagatgaag atggaagtgc caggcaggct
     2041 gatctgcaag gcggctggta tcattttgaa gcaaaatggt tttcttcttc aatatttgaa
     2101 ggtagagtga ttccatgtat ttcggcagag gctcagaaaa tttttcatag tggatatgaa
     2161 ttgagagaag ttgatcatat cgatctgaaa aatcttgaag cgctgaagag agcaatctat
     2221 ttaatcaccg gggttatggc gtccggaaaa tctacagtcg ctcaattgct tgccttaaaa
     2281 atggaaaaag gagtgcatct gcggggcgat atatttcgta aaatgattgt tgccggacgg
     2341 gctgacatgt ccgtacagcc ctccgaagaa gctatccgac aactgcatct gcgttaccgc
     2401 ttggcagctg aaacggcaaa aacctattat gacagcggtt tttccgttgt tttgcaggac
     2461 aattattacg gtgaggaact gccccgcatg ttgaagatgc tagagaatta ccctgttcat
     2521 gtaacagtgt tgtgtccgga tgtggagaca gtaaaaaggc gtgaaaagat gcgagggaaa
     2581 actggctata ccggtttttc actagaagct ctgcatgcgg atttcatgag aaaaacccca
     2641 agactcggat tctggctgga caactcagag ctgacgccgg aacaatccgc tcgggatatt
     2701 ctattgcact tcggtgaata acggtgaata agtcacatta gattgaacac tattgataat
     2761 tttaatgtcc gaaagggagt aacataaatg aataatatac tgattgttta ttattcactt
     2821 tttcataata cggcgaattt agcattggaa atagcagtac aaacaggtgg aacattaaga
     2881 gaattgattc ccgatacgaa ttattccttt gaatataata ctgctgctaa agaagtaaga
     2941 aatcagattt ctcgaggctt ttgtccaaaa ttaatatctg gaaatgaatc aatagacgat
     3001 tatcaagcaa tatttatagg atcacccaac tggttcaaaa ctttagctcc gccagttatg
     3061 agtttcctta gactgaatga ttttacgggt aaaacaatca tacccttttg cacacacggt
     3121 ggtggaggat tcggccaaat tgcaaatgat atagcaaaag agtgcccgaa gtcgaaaata
     3181 ctacccggta ttgctgtaac tggtactgtc acatcggaag aagtcacgaa ttggcttaag
     3241 atgatagaat atgaattata aaatcccgtt tcaaggagga atcctgtatg cagtatggtc
     3301 ccgacccaaa cgccatttat ccaaatgaaa agataaagag cgtatgctac atcaagaata
     3361 ttatcacccg ccctaatatc at
//
LOCUS       DAOPYB010000272         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_37981_length_3376_cov_10.529961, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000272 DAOPYB010000000
VERSION     DAOPYB010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37981_length_3376_cov_10.529961"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..756
                     /locus_tag="PKH58_12890"
     CDS             115..756
                     /locus_tag="PKH58_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89968.1"
                     /translation="MKWFNFTVLIFLCTACVRNNNTTTQQIKKTVNILAKSESLSLIG
                     TDEFVLFWNKFSGALLANDTNVLSNSIDDKFHGYCGSLLDISNISKMDFKSDSIISKS
                     RFLKEFKSSLNPAYLQLIKQYKILEDIKHVRTLEDAKNHYFCIKTVDKNIYNVSTYLA
                     HENKNEKVIFKMSYMNDDKRYETKNIDLIFHKRNNETKLCEIDFYYSYIDEER"
     gene            1028..1801
                     /gene="lptB"
                     /locus_tag="PKH58_12895"
     CDS             1028..1801
                     /gene="lptB"
                     /locus_tag="PKH58_12895"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNX89969.1"
                     /translation="MVLRTEHLFKKYGKRTVVNDVSIQVQQGEIVGLLGPNGAGKTTT
                     FYMTTGLVTPNSGKIFLDDEEITGLPVFRRAQKGIGYLAQEASVFRKMTVEDNISSIL
                     EMTKFSKEYQKEKLETLIAEFNLGHVRKNIGDRLSGGERRRTEIARCLAIEPRFIMLD
                     EPFAGVDPIAVQDIQDIVWQLKDKNIGILITDHNVDETLSITDRAYMLFEGKIMFQGT
                     SEELADNPIVREKYLGRDFKLKVKTRVDTVVNPNIEGFI"
     gene            1842..2753
                     /locus_tag="PKH58_12900"
     CDS             1842..2753
                     /locus_tag="PKH58_12900"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNX89970.1"
                     /translation="MNAKLTIIGSGSALPTKNNFPTSQLLEFRNKQFLIDCGEGTQIR
                     MRQMNLKNSHIGHIFISHLHGDHCFGLMGLISSFGMLNHTSDIHIHAHPDLEKLMKPQ
                     LKYFCEELPFQVIFCPVNPAVNEVIFEDRTVQVSSIPLKHRVPCCGFLFEEKSSDRHI
                     NREMIDFYRVPVWQIGKIKQGEDFTTEEDEIIPNDKLTLPPTPPIRYAYCSDTAYSEK
                     IIPIIEEVDLLFHEATFMEDEIYRARATQHSTARQAAEIAKKANVKKLIIGHYSARYS
                     HKTALLNEARQVFERTELGEDMTSYEF"
BASE COUNT         1123 a          697 c          682 g          874 t
ORIGIN      
        1 gaatgcaatt ggctacgccg aatgctgttt cccagcggcg tgctgtacta aggtaatata
       61 tttatttaag cacatattcg aaaggtcggg ttaagatttt aattttatgt cattatgaag
      121 tggtttaatt ttacagtact tatatttttg tgtaccgctt gcgtaagaaa caacaataca
      181 actacacaac aaatcaaaaa aacggtaaac attttagcaa agagtgaatc tctttcttta
      241 attggcactg atgaatttgt gttgttctgg aataaattca gtggagcgtt gcttgcaaat
      301 gacacgaatg ttttgagtaa ctccattgat gataaatttc acggctattg cggatcttta
      361 ttggatattt ccaacataag taaaatggat tttaaaagtg acagtattat ttctaaaagc
      421 cgttttttga aagaatttaa aagctcatta aatcctgctt atctacaatt gattaaacaa
      481 tataaaattc ttgaagatat aaaacatgta agaactttgg aagatgctaa aaatcactat
      541 ttctgtataa aaacagttga taaaaacatc tacaatgtct ctacatattt agcccatgaa
      601 aataagaatg aaaaagtcat ttttaagatg agctatatga atgatgataa gaggtatgaa
      661 acaaagaata tcgatttaat atttcacaaa aggaataacg agacaaaact ctgtgaaatt
      721 gatttttatt attcttatat tgatgaagaa aggtaatgca cccacaccct ttcgtatatt
      781 cagaataaat gagcaaaaac cagtgagacg gaacctaccc aaccagtctt aaattcagac
      841 aatccggctc aaccggcagc ttattttctt aaaaaatcac agaatgactt gctgctctgt
      901 gttaattcat tcagaatttt gtacttttgc tccattaaaa atattcagct aaatggaagc
      961 agacatcgta aaggaaaatt ttaccgaaat cgaaacaata acagaacttg caccggaaag
     1021 caggtctatg gtactccgca ccgaacatct ctttaagaaa tacggcaaac gaaccgttgt
     1081 gaatgatgta tcgatacagg tgcaacaggg cgaaattgtg ggtttgctcg gtccgaacgg
     1141 agccggaaaa actaccacgt tctacatgac taccggactg gttactccca attccggaaa
     1201 aattttcctg gacgacgagg agattacggg gctgcctgta tttcgccgtg cgcagaaagg
     1261 tataggttac ctggctcagg aagcttccgt tttccgcaaa atgactgtgg aagataatat
     1321 cagctctatt ctggaaatga ccaaattcag caaggaatat cagaaggaaa aacttgaaac
     1381 gctgattgcc gaatttaacc tcgggcacgt gcgcaagaat atcggcgacc ggttgtcggg
     1441 aggtgagcgg cgacgtactg agatagcacg ctgtctggcc attgaacccc gcttcatcat
     1501 gctcgacgaa cccttcgcgg gcgttgaccc cattgccgtg caggacattc aggatattgt
     1561 gtggcagctg aaagacaaaa atatcggcat cctcattacc gaccacaacg tggacgaaac
     1621 actcagtatc accgaccgcg cttatatgct tttcgaggga aaaatcatgt tccagggaac
     1681 ctccgaggaa cttgccgaca accccatcgt gcgtgagaaa tacctcggac gcgacttcaa
     1741 actgaaagtg aagacgagag tggatactgt ggtaaatccg aatatcgaag gttttattta
     1801 attcgcccct ctgactttca agctattgat acacctcagg aatgaacgca aagcttacaa
     1861 tcatcgggag cggctccgca ttgcccacga aaaacaattt cccgacttca cagttgctgg
     1921 aattccgaaa caagcagttt ctcattgatt gtggcgaggg cactcagata cggatgcggc
     1981 agatgaacct caaaaacagc catatcggtc atattttcat ctcgcacctc cacggtgatc
     2041 attgcttcgg gttgatggga ctcatttcgt ccttcggaat gctcaaccac acctccgaca
     2101 tacacatcca cgcgcatccc gacctggaga aactcatgaa gccgcaactg aagtatttct
     2161 gcgaagaact cccctttcag gtgatattct gcccggtcaa tcccgctgtc aacgaagtta
     2221 ttttcgagga ccgcaccgtt caggtttcgt ccataccgct aaagcacaga gtgccctgct
     2281 gcggttttct tttcgaagag aaatcgagcg acaggcatat caaccgcgag atgattgatt
     2341 tttacagggt tcccgtgtgg caaatcggga agataaaaca gggcgaagat tttacgaccg
     2401 aagaagacga aatcattccc aatgacaaac tgaccctgcc tcccactcct cccatccgtt
     2461 atgcttactg ctcggataca gcatattccg aaaaaataat ccccattatc gaagaggtgg
     2521 atctgctgtt ccacgaagcc acctttatgg aagatgagat ataccgcgcc agagccactc
     2581 agcactcaac cgcgcggcag gctgccgaga tagccaaaaa agcaaatgtc aaaaaactga
     2641 ttatcggaca ctactccgcc cgctacagtc acaaaacagc gttgctgaat gaagcgcggc
     2701 aagtttttga acggaccgaa ttgggggaag atatgacgtc gtacgagttc taaaatttca
     2761 aatcccccta aaaattcagc aacttggttt tgctatttgg gataaaaaac aacaataact
     2821 tcaataagaa aaagctgaca tgtactttga ctattttgat ttgaaaaacg atatataaat
     2881 tggatatgaa aacaattatc aatatcttgt gaaatatctg aaagtaagcg ttcattgtaa
     2941 aaaaaaacgc acacagacag ctaattacaa aataaagaat atgaaacgaa ttaatcagga
     3001 taaaataata tgtaataagt gtttctgtag caattcaaac attctgcccc tctcaaactt
     3061 cggtgtaggt ggttcaggaa agatgcacgc aatcacttac tgaatatatt acctcaaacg
     3121 ttgcgctaca tatcttgata caaaaatcag atacggcagg tcgggaacta aaaacttgaa
     3181 ctaacattat aaaaaccaaa agaaaatgga aacaaaagaa aattgtgaaa attgtaagat
     3241 gcgtgcaaaa tacgatgcaa aacccaattc atttgcagga cgtttttggc ggtggcacat
     3301 taattggtgt ccgggatgga aaaagtatat ggcctcttta gacgaaaata aaagggcaaa
     3361 aatcaaagag aattat
//
LOCUS       DAOPYB010000273         3373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_38031_length_3373_cov_10.090717, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000273 DAOPYB010000000
VERSION     DAOPYB010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38031_length_3373_cov_10.090717"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKH58_12905"
     CDS             <1..741
                     /locus_tag="PKH58_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNX89971.1"
                     /translation="DGDRLGFPVFPLDWEDPKTEAVSSGYREAGYFPEAVVNFLALLG
                     WNPGTEQELFSMQELIEQFSLQRVSKSGAKFDYEKGKWFNHKYLQMKPNEELADIYQH
                     ILVEKDIIEDSEKVSKVVGLIKERANFVNDLWDQSYYFFQAPTEYDEKSVKKRWKQDT
                     PERMAELIEVIKTIDDFSSANQEAVICKWIEEKGYGMGAILNAFRVAIVGAAIGPHMF
                     DITEIIGKEETIERINRAISFLSKTEDE"
     gene            734..2074
                     /locus_tag="PKH58_12910"
     CDS             734..2074
                     /locus_tag="PKH58_12910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX89972.1"
                     /translation="MNNNQVVHNPESSNKKKALIITYYWIPSGGAGVQRWVKFVKYLR
                     TYGIEPVVYTPENPEYPSEDLSLRKDIPADIEVLKTPIWEPYNVYRNLFGKKGEKINA
                     GFISEGKKTGWKEKFSIWIRGNFLIPDPRKFWIKPSVRFLKEYLQKNQIDTIISTGPP
                     HSMHMIGLGLKKQLPELTWIADFRDPWTNIDFYKELNLTYLADRRHKNLEKRVVKNAD
                     CVIVVGGEMVREYTSLKPKRIELITNGYDEADISDVKVEMDRLFTISHIGTLNAARNP
                     ITLWKVLSEMCREDEDFRTNLQIQLVGKVDFSVLEDIRKYNLENNLLKIDYMAHSDAI
                     ARQQASRLLLLLVNNTANAKGILTGKFFEYLAAKRPILAIGPVDGEVAQVLKETSAGC
                     IADFEDEVQTRKIVTEYYEQYKRGELTVSPKSIEKYSRRELTGELVRIIEDIDK"
     gene            2071..3141
                     /gene="wecB"
                     /locus_tag="PKH58_12915"
     CDS             2071..3141
                     /gene="wecB"
                     /locus_tag="PKH58_12915"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNX89973.1"
                     /translation="MKNIITVVGARPQFVKAATLSRQFGLQDVNEKIIHTGQHYDANM
                     SEVFFEEMEIPRPAYQLDIHGLNHGAMTGKMLRGIEEILINEKPDGVLVYGDTNSTLA
                     GALAAVKLHIPVVHVEAGLRSFNMQMPEEVNRILTDRISDLLLCPTDTAVDNLKSEGF
                     DNFHKRIMKNGDVMQDAALYYARKAEQKSDILQKKNLSDFVLATIHRQENTDNPENLR
                     GIINGLNEINRMKAVVVPLHPRTRNILKQQNIIPEFTIIDPVGYFDMIMLLKSCKMVI
                     TDSGGVQKEAFFFGKQCVTMREETEWVELVKYGYNILAGSNSAKIIAAFCESLFRKSD
                     FTINLYGQGQAAEMAVREIIAL"
     gene            complement(3201..3368)
                     /locus_tag="PKH58_12920"
     CDS             complement(3201..3368)
                     /locus_tag="PKH58_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX89974.1"
                     /translation="MAYVITEDCIACGTCISECPVEAISEGDIYVINPDICTDCGTCA
                     DVCPSEAIHPA"
BASE COUNT         1096 a          626 c          779 g          872 t
ORIGIN      
        1 gatggcgaca ggctgggatt tccggtattt ccgcttgact gggaagatcc taaaacagaa
       61 gctgtatctt cagggtatcg cgaagccggt tattttcccg aagcagtggt aaattttctg
      121 gcattactcg gatggaatcc cggcacagaa caagaattat tctcgatgca ggaacttatc
      181 gaacaatttt cgctacagcg tgtaagcaaa agcggtgcaa aatttgatta cgaaaaaggt
      241 aaatggttca atcataagta ccttcaaatg aaacctaatg aagaacttgc ggatatctat
      301 cagcatattc tagttgaaaa ggacattatt gaagattctg aaaaggtgag taaagttgtc
      361 gggctaatta aagaacgtgc taattttgtg aacgatttgt gggaccagag ctactatttc
      421 ttccaggcac caaccgaata cgatgaaaaa tcggttaaaa aacgctggaa acaagatact
      481 cccgaacgca tggccgaact aattgaagtg ataaagacca tcgatgattt ttcatccgca
      541 aaccaggaag ccgtcatatg caaatggatt gaggaaaaag gctatggaat gggagctata
      601 ctgaacgcgt tccgtgtagc aatagtggga gctgcaatcg gaccgcatat gttcgatatt
      661 accgaaataa tcggaaaaga agaaactatc gaacggatta accgtgcaat cagtttcttg
      721 agcaaaacgg aagatgaata ataatcaggt tgtacataat cctgaatcat ccaataaaaa
      781 gaaagcattg atcatcactt attattggat acccagtgga ggtgcaggtg tacagcgctg
      841 ggtgaagttt gtgaaatatc ttcgtactta cggaatcgaa ccggttgttt atactcccga
      901 aaatcctgaa tatccttctg aagacctttc gctccggaag gatatacctg ccgatattga
      961 ggttttgaaa acaccaatat gggaaccata caacgtgtat cgtaatcttt tcggaaaaaa
     1021 aggagagaaa ataaacgcgg gatttatttc agaaggtaag aaaacaggct ggaaagaaaa
     1081 gttttcgatc tggatacgtg gaaattttct gattcccgac ccgagaaagt tctggataaa
     1141 accttcggtc agatttttaa aggaatatct gcagaaaaat cagattgata ccatcatctc
     1201 aacaggtccc ccgcattcga tgcatatgat tgggctggga cttaaaaaac aattgcccga
     1261 attgacctgg atagccgatt ttcgtgatcc ctggacaaat attgattttt acaaagaact
     1321 taatctcact tatcttgctg acagaaggca taaaaatctc gaaaaaagag ttgtaaaaaa
     1381 tgctgattgt gtaatagtgg tgggtggtga gatggttcgt gaatatacca gtctgaagcc
     1441 gaaacgtatt gaactgataa caaacggtta cgatgaagcc gatattagcg atgtaaaggt
     1501 tgagatggac cggttgttta ccatttcgca tatcggaacc ctgaatgctg cccgaaaccc
     1561 cataacactc tggaaagtgc tgagcgaaat gtgcagggag gacgaagact tcagaaccaa
     1621 tcttcaaatt cagttggtcg ggaaagtaga tttctcggtt ttggaagata tcagaaaata
     1681 taatctcgaa aataatctgc tgaagataga ttacatggca cattccgatg ccattgcccg
     1741 tcagcaggca tcccggttgc tgttgctgtt ggttaacaat acagctaacg caaaaggaat
     1801 actaaccgga aaattttttg aatacctggc agccaaaaga cccattctgg ccatcggacc
     1861 tgttgatgga gaagttgcac aggtattgaa ggaaacttca gcaggatgta tcgcggattt
     1921 tgaagatgaa gtgcagacga ggaaaattgt cacggaatat tacgagcaat ataaacgcgg
     1981 tgaactaacc gtgtcgccca aatcaataga aaaatactcc cgccgtgaac tgaccggtga
     2041 gttggtacgg atcatagaag acattgataa atgaaaaata taattaccgt agtcggagca
     2101 cgacctcagt ttgtcaaagc agctacttta agcaggcagt ttggtttaca ggacgtgaac
     2161 gaaaagatta ttcataccgg tcagcactac gatgccaaca tgtcggaggt tttcttcgag
     2221 gaaatggaga tacccagacc tgcttatcaa ctggatattc atggattgaa tcatggggcg
     2281 atgaccggga aaatgcttcg gggaattgaa gaaatcctga taaatgaaaa acccgatgga
     2341 gtacttgttt acggcgacac aaactcaact ttggccggag cgctggcagc agtcaagctg
     2401 catattccgg tggttcatgt cgaagccggt ttgcgttcat tcaatatgca gatgcccgaa
     2461 gaagtcaata ggatacttac agaccgcatc tcggatttgc tgttatgtcc gaccgacaca
     2521 gctgtagata atctgaaaag cgaaggtttt gataattttc ataaacgaat catgaagaat
     2581 ggcgatgtga tgcaggatgc agcgctttat tacgcccgca aagccgaaca gaaatcagat
     2641 atcctgcaaa agaaaaatct ttcggatttt gtgttagcca ccatacaccg tcaggagaat
     2701 accgataatc cggagaactt gcgtggaata atcaacgggc tgaatgaaat aaacaggatg
     2761 aaggctgtgg ttgttccgtt gcatccacgt acccgcaata ttctgaagca acaaaacatc
     2821 atacctgaat tcacgattat tgatccggtt ggatattttg acatgattat gttactaaaa
     2881 agctgcaaga tggtgataac agatagcggc ggagtgcaaa aggaagcctt ctttttcgga
     2941 aaacagtgtg ttacgatgcg tgaagagacc gagtgggttg aacttgtgaa gtatggttat
     3001 aatatacttg ccggaagtaa ttctgcaaaa attattgcag cattttgcga aagtctgttc
     3061 cggaaatctg attttaccat aaatctttat ggtcagggac aagcagctga aatggccgta
     3121 agagaaataa tcgcattata ataaaaaatc ctattcgccg aaacgaatag gattttattt
     3181 tttttgatta gcgtattgtg ttacgctgga tgaattgctt cagaaggaca aacatcagca
     3241 caagtgccgc agtctgtaca tatatcagga ttgataacat agatatcacc ttctgaaatt
     3301 gcttctacgg gacattcgct gatgcaagtg ccgcaagcaa tacaatcttc tgtaattacg
     3361 taagccattt taa
//
LOCUS       DAOPYB010000274         3356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_38298_length_3356_cov_10.698273, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000274 DAOPYB010000000
VERSION     DAOPYB010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38298_length_3356_cov_10.698273"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..644
                     /locus_tag="PKH58_12925"
     CDS             78..644
                     /locus_tag="PKH58_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89975.1"
                     /translation="MAFFSKEKRFQLAIHSLEDGYFRNKFENDIKNFSGNSGIGVISD
                     TEAQPIVVIQGKKGVFLDDSIVRGTQLKDNVHDLHEAGIKEVNMRIACPPLTYPCEFL
                     NFSRSRSSLELATHKAVRELENKEGVDMKEYSDCNSEKYRAMVEKIREHLDLTSLSFQ
                     KLDDLVEAIGLSKEKLCTHCFDNSSYFD"
     gene            complement(667..2196)
                     /locus_tag="PKH58_12930"
     CDS             complement(667..2196)
                     /locus_tag="PKH58_12930"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNX89976.1"
                     /translation="MENSQHIGFDNDKYLQEQSAAILERVSRFNDKLYLECGGKILYD
                     YHAARVLPGFDPNVKMRLLQKLKDKIDVIMCIYAGDIERNKIRADFGIPYDVDALKSI
                     DDLKWFGINVAAVVITRYQDQPAAAAFKNKLEMKGINVYLHYPTNGYPTDVEKIVSDG
                     GYGANEYIKTTRPIVVVTGPGPGSGKLATCLCNLYHEYKKGVKAGYAKFETFPVWDLP
                     LKHMVNVAYESATADLKDVNMIDTHHLKAYSTEVINYNRDIEAFNLLKKILEKITGGE
                     SMYLSPTDMGVNRISSGIVDDQIISEASYQEIIRRYFRSNVDFTMGLASRETLDRSLE
                     IMNEVGATETDRKVVLPARKAAVAAQKEEGKGYGGIYCGSAIQLHDGTIITGKNSQLL
                     HASSAMILNATKYLAGLPDSMVLLPESIIDSVTHLKKDILNGKKVSLDVEETLIALAI
                     SATSNPAAKMALDKLTELHGCEMHSTHIPTAGDEAGFRKLKVNLTCDPEFSSKRLFVS
                     E"
     gene            complement(2456..2923)
                     /locus_tag="PKH58_12935"
     CDS             complement(2456..2923)
                     /locus_tag="PKH58_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89977.1"
                     /translation="MKTIYILFLMSSLMAFPQISATEPKLVDIATDETIPPDISVNTL
                     KVFEIKSNQARCSFSIHGTTVSERGVYYSQSPDAELTGKKSMAPGNTNNGSVIIAGLS
                     PGTIYYVRAYARSGSDIVYGNELSFKTLSVEEKTKTTSNAGQKVEKKESNVSK"
     gene            complement(2959..>3356)
                     /locus_tag="PKH58_12940"
     CDS             complement(2959..>3356)
                     /locus_tag="PKH58_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX89978.1"
                     /translation="SNVRIAVGAPDTRSKLITEGKLVTYGIYFDVNKDVVKAESFGTL
                     KSIADVLKENPDVRIKIVGHTDSDGDNAKNLDLSQRRAKSVMNELVNVFGIEASRMET
                     DGMGETKPVAPNDTPVNKALNRRVEFIKL"
BASE COUNT          881 a          749 c          692 g         1034 t
ORIGIN      
        1 tacaacactt ttgtatttct tcaatctaca tatatattga attgtaatac gattacattt
       61 aggcacaaaa agagccggtg gcattctttt caaaggaaaa aagatttcag ctcgccattc
      121 acagcctcga agatggttat ttcaggaata agtttgagaa tgatatcaaa aacttctcgg
      181 gtaacagcgg cattggcgta atcagcgata ccgaagctca gccaatcgtt gttattcagg
      241 gcaaaaaggg agtttttctc gacgattcaa ttgtacgcgg tacgcaactt aaagataacg
      301 tacatgacct gcacgaagca ggtattaaag aagtgaatat gcgcatcgcg tgtccgccgc
      361 tcacctaccc ctgcgaattc ctgaatttca gccgttcgcg ttcgagcctc gaactggcta
      421 cacataaagc tgtcagagag cttgaaaaca aggaaggtgt ggacatgaag gagtattcgg
      481 actgtaacag cgagaaatac cgggcaatgg ttgaaaagat acgggaacac ctggatctga
      541 cctctctctc ctttcagaaa ctcgatgacc tggttgaagc catcgggtta tcaaaagaaa
      601 aactttgcac gcactgtttt gacaattcca gctatttcga ttaacagaac aaaaaaacaa
      661 tcagttttat tcgctgacaa acaatcgttt gcttgagaat tcgggatcac aggtaaggtt
      721 taccttcagt ttgcggaaac cggcttcgtc accggcagta ggaatgtgtg tggaatgcat
      781 ttcgcaacca tgcagttcgg tcagtttatc aagtgccatt tttgccgcag gatttgacgt
      841 agcgctgatg gctaacgcga tgagcgtttc ctctacatcg aggctgactt tcttgccgtt
      901 caggatgtct tttttcagat gagtaaccga atcgatgatg ctttcgggca gaagtaccat
      961 gctgtcggga agtccggcaa gatatttggt tgcattgagt atcatggcgg aagacgcgtg
     1021 taacagctgc gaatttttac cggtaataat ggtcccgtcg tgcaattgaa tggccgatcc
     1081 acaataaatt cctccgtaac ctttcccctc ctctttttga gcggcaacag cggctttgcg
     1141 ggcaggcaac accactttac ggtctgtttc ggtggctccg acctcattca tgatttcgag
     1201 cgaacggtcg agtgtttctc tggatgcaag tcccatcgta aagtccacat tgctcctgaa
     1261 atacctgcgg ataatttcct gataggatgc ttcggaaata atctgatcgt ccacaatccc
     1321 cgaactaatg cggttgacgc ccatatcagt cggtgaaaga tacatggatt ctccaccggt
     1381 aattttttcg agaatttttt taagcagatt gaacgcctca atatctcggt tgtagttgat
     1441 cacttcggta ctataggctt tgagatgatg ggtatcaatc atatttacat ccttcagatc
     1501 ggcagtagcc gattcgtagg ccacattcac catatgtttg agcggaagat cccagacagg
     1561 aaatgtttca aattttgcat atccggcttt aactcctttt ttatattcgt gatacaggtt
     1621 gcagaggcag gttgccagtt ttccgctgcc gggcccgggt cccgtaacca ccacgatagg
     1681 ccgggtggtt ttaatatatt cattggctcc gtagcctcca tcgctcacaa ttttttccac
     1741 atcagtcgga tatccattgg tagggtaatg cagatataca tttatcccct tcatctccag
     1801 cttgtttttg aaagcagccg ctgccggttg atcctgatag cgggtaataa ctactgcggc
     1861 cacgttgata ccgaaccact tcaggtcgtc aatgcttttc aacgcgtcca catcgtaggg
     1921 aattccaaaa tcagcccgga ttttattgcg ttcaatatct ccggcataga tgcacatgat
     1981 gacatcaatc ttgtctttca acttttgtaa aagcctcatc ttcacattcg gatcgaaacc
     2041 gggtaatacc cttgcagcat gataatcata aagtatttta cctccgcatt caaggtataa
     2101 cttatcgtta aatctgctta ccctttcgag tattgccgct gactgttcct gaaggtattt
     2161 atcgttatca aaaccaatgt gctgcgaatt ttccattttt tcccgtattg atgattgatt
     2221 ttttattaca atctgcaaaa ttactgaaat tttaattctc tctattcctc cgtgctcaaa
     2281 gaaaattgaa tatttttatt catctaaaca tcatcttaat cagcgctgat tgctatccca
     2341 tgaattccaa atgtttattc agttaaatca atcagatgaa aaaaagaaga gcctgacgga
     2401 taaaaaccgg aggctctctt ttgctgctga agcatttata atgtttgcaa tgacattatt
     2461 tgctcacatt cgactctttc ttttccacct tttgacctgc attactggtg gttttggttt
     2521 tctcttctac tgacagtgtt ttgaaactta attcattgcc gtaaacaata tcagaaccgc
     2581 ttctggcata agccctgaca taataaatgg ttccggggct aagtcctgca ataatgacgc
     2641 ttccgttatt tgtattaccg ggagccattg atttctttcc ggtaagttct gcatcgggcg
     2701 attgcgaata atagacacct ctttcgctga cggtagttcc atgaatacta aaactgcatc
     2761 gggcctgatt tgattttatt tcaaaaacct tcagtgtgtt gacgctgata tcgggtggaa
     2821 ttgtttcgtc tgtagcgata tccaccagtt taggttccgt agcgcttatc tgaggaaaag
     2881 ccatcagaga actcatcaga aacagaatat agattgtttt cataattaat ttttgtatta
     2941 gaccggttaa ttaatttctt ataatttgat aaattccact cttcggttca gtgccttatt
     3001 gactggcgta tcgtttggag caacgggttt ggtttcgccc atgccatcgg tttccattct
     3061 cgaagcttca atgccaaaca cgttaaccag ttcattcatc actgattttg cccggcgttg
     3121 cgataaatcg agatttttgg cattgtcacc atcgctgtcg gtatgaccta cgattttaat
     3181 tcgtacatcc ggattttctt tcagtacatc ggcaatgctt ttcagcgttc cgaacgattc
     3241 agcttttacc acatccttgt tgacatcgaa ataaataccg taggtaacca gtttaccttc
     3301 tgtaattaac ttactgcggg tatcgggagc gccaaccgct atccgcacat tgctaa
//
LOCUS       DAOPYB010000275         3339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_38584_length_3339_cov_8.617540, whole genome shotgun sequence.
ACCESSION   DAOPYB010000275 DAOPYB010000000
VERSION     DAOPYB010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38584_length_3339_cov_8.617540"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..585)
                     /locus_tag="PKH58_12945"
     CDS             complement(61..585)
                     /locus_tag="PKH58_12945"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNX89979.1"
                     /translation="MSYWCILRIASNKEKQIIEQIDKRLISAKIDKNCIRMVAPVQKI
                     EIMKNGKKVTKTKSLYPGYMYIEFNEGYSKELYEIITTSNHVYGFIPGTLTPSEIENI
                     LKYTDDYKEKEIVSDEKYLIGEKVNIIDGPFTNYAAKIVDFHEDKKRLKVSVTVFGRD
                     TSVELKTSQVKKFI"
     gene            complement(617..790)
                     /locus_tag="PKH58_12950"
     CDS             complement(617..790)
                     /locus_tag="PKH58_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89980.1"
                     /translation="MTDNNKNKFENIIILLLLFGLFAWLWEQINKSKYHKEIKIALAI
                     GFIILLITSYCVK"
     gene            complement(817..1674)
                     /locus_tag="PKH58_12955"
     CDS             complement(817..1674)
                     /locus_tag="PKH58_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89981.1"
                     /translation="MQIIQNTHTTYQPNILTYYKTIEHLPNVLNFEQLLQQFDSLKML
                     HNASYFRLMKKWESAEMPHPPVVFGLASGAFFTAGKIVFYYHRNGQARLKGLPYSSMS
                     LYLGNKNSKFTDFLISRFSQADWKVIADFSNYCTNNYKINQARKTERKARQLETLQDL
                     KVKLTERMPILEYEQLLDKYNHFSLFEADRNWNRFHWYRKALASWKNGNMPYPPILFG
                     LESGLMFGKTGFICDYRADQGECESKRYYLKTDYRNINHPDFENRLSKFLTTNDMDVL
                     KELGKMLME"
     gene            complement(1726..>3339)
                     /locus_tag="PKH58_12960"
     CDS             complement(1726..>3339)
                     /locus_tag="PKH58_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX89982.1"
                     /translation="ELYESELNPETTAKIKTESLQLANYLDVTEQQAWFFSIIYVQQS
                     MFFSCDLNDILNELSIKTSKFVKYRSDIVTLIDKKIVRSELVRSNKSKVARANFKYMM
                     INETILNAILHNISLDESNKLDQLDIYEFCSAVSDFIEERAQDVISTDNLLGEVSSLE
                     MHNSHIEAIDKLTEMEISLHDRVLLYEMCDDMVRGGDTALEKTIRYIYDSQKQKIKVI
                     RAITENTSKLLEKNLIVVNKANFVGDVSISLTTTAIDILLGQDAEMFIKKQTLKDVIS
                     VDSIAFKQLYFEEKMQKQIDFIQASLSQDKFVDMQSRLQELAMPSGVAAVFYGAPGTG
                     KTESVYQIAKATGRDIMYVDISQTKSMWFGESEKRIKAVFSDYARVCRKSKLKPILLF
                     NEADAVLGKRKDNNASNVAQTENAIQNIILEQMEKMDGIIIATTNLVDNLDAAFERRF
                     LFKIKFEMPTTEATKQIWKNKLSWLDDENATTLALRYRFSGGEIDNIARKATINEVIT
                     GNRTTLNEIMEYCENERLLSKNLPKLGFN"
BASE COUNT          899 a          644 c          597 g         1199 t
ORIGIN      
        1 ttcacacccc aaatatacaa atttatttgg agtgtgaaga tttgtaaata aattattttt
       61 ttaaataaac ttcttaacct gtgaagtttt taattcaaca gaagtatctc ttccaaaaac
      121 ggtaacagag actttcaatc ttttcttatc ttcatggaaa tcaactatct ttgctgcata
      181 gttagtaaac ggtccatcga taatatttac cttttcgcca atcaggtatt tttcatctga
      241 tacaatttct ttttctttgt aatcatctgt gtactttaga atgttttcta tttcggaagg
      301 tgttaacgtt ccaggaataa atccatacac atggttcgaa gttgtgataa tttcgtatag
      361 ttcttttgaa taaccctcat tgaattcaat atacatatat cccggataaa gacttttagt
      421 cttagttacc tttttcccat ttttcataat ctctattttc tgaaccggag caaccattcg
      481 tatgcagttt ttatcaattt ttgctgaaat taatcgttta tcaatctgtt caattatttg
      541 cttttctttg tttgaagcta ttctgagaat gcaccaatat gacatattta tttggattaa
      601 aaaattagta taaatattat tttacgcaat agctggtaat cagcaaaata ataaagccaa
      661 tggcaagagc aattttaatt tctttatgat acttactttt gtttatttgt tcccataacc
      721 aggcgaacaa gccaaacaga agcaacagga tgattatatt ctcgaatttg tttttgttgt
      781 tgtcagtcat acaatcttat tttttaaggt ttatttttat tccatcagca ttttccccag
      841 ttcttttagc acatccatat cattcgttgt aaggaatttg cttaatctgt tttcgaaatc
      901 aggatgattg atgttacggt aatctgtttt caggtaatat cttttggatt cgcattcacc
      961 ctgatctgct ctgtaatcac aaataaatcc ggtcttgcca aacatcagtc cgctttccaa
     1021 tccaaaaagg attggtgggt aaggcatatt tccatttttc catgaggcca gcgcttttcg
     1081 ataccagtga aaacggttcc aattcctgtc agcttcaaac aggctgaaat ggttgtattt
     1141 atccagcaat tgttcatatt caagtatcgg cattctttcg gttagtttta cttttaaatc
     1201 ctgcaaagtt tcaagctgtc ttgcttttcg ttcagttttt cttgcttgat taatcttgta
     1261 gttgttggtg cagtaattac tgaaatcggc aattactttc cagtcggcct gactaaaccg
     1321 actgatcagg aaatcggtaa atttgctgtt cttgttgccc aggtacaaac tcatgctgct
     1381 gtaaggcaaa ccttttaatc tggcttgacc attcctgtgg taataaaaaa caattttgcc
     1441 ggcagtaaaa aaggctccgc tcgctaatcc gaaaacaacg ggaggatgcg gcatttcagc
     1501 gctctcccat tttttcatca accggaagta agaagcattg tgcagcattt tcaggctatc
     1561 aaactgttga agcagttgtt caaaattcag tacattgggc aagtgctcaa tggttttgta
     1621 gtatgttaag atattaggtt gataggtagt gtgcgtgttt tgaataattt gcatatttat
     1681 gattttaggg tttatgtggg ttaaaaagaa aattttcacc ggagtttagt taaaccccag
     1741 tttcggaaga tttttactca gtagtcgttc gttttcacag tattccataa tttcgttcag
     1801 ggttgtccgg tttcccgtaa ttacctcgtt gatggtagct ttgcgggcga tattatcaat
     1861 ttcgccaccc gaaaaacgat aacgcaaagc gagtgtggtg gcattttcat cgtccagcca
     1921 gcttaattta tttttccata tttgtttggt agcctctgtt gtaggcattt caaatttgat
     1981 tttaaacagg aacctccgtt caaaagcagc atccaggtta tcaaccaaat tggtagtggc
     2041 aattatgatc ccgtccatct tttccatttg ttccaggatg atattctgga tggcattttc
     2101 ggtttgcgcc acattggagg cattgttgtc cttgcgtttt cccagcacgg catcggcttc
     2161 gttgaaaagc aggatgggtt tcaacttgct tttcctgcat acccgggcgt agtcgctaaa
     2221 cactgctttg atccgttttt cactttcacc aaaccacatg gattttgtct ggctaatgtc
     2281 cacatacata atatcacgtc cggtagcttt ggcaatttga tataccgatt cggtttttcc
     2341 ggttcccgga gcaccataaa acacagcagc tacaccactg ggcattgcca gttcctgcaa
     2401 acgggattgc atatccacga atttgtcctg cgagagactt gcctggataa agtctatctg
     2461 cttttgcatt ttttcttcaa agtaaagttg tttgaaggca attgaatcaa ccgaaataac
     2521 atctttgagt gtttgtttct tgataaacat ttcggcatcc tgtcctaaaa gtatatcaat
     2581 agcagttgtt gtcagagaga tactaacatc tcctacaaaa ttagctttgt ttactactat
     2641 caaatttttt tccagcagct tgcttgtatt ttcagttatc gcccttataa ctttaatttt
     2701 ctgtttttgc gaatcatata tgtatcggat ggttttttcc aacgctgtat caccgcccct
     2761 taccatatcg tcgcacattt cgtacagtag cacacggtcg tggagtgaaa tttccatttc
     2821 tgttaatttg tcgattgctt caatatggga attgtgcatt tccaacgaac tcacttcacc
     2881 caaaagatta tctgtactga tcacatcctg agctctttct tcaataaagt ctgatacggc
     2941 gctgcaaaac tcatatatat ccagctggtc taacttattg gattcgtcta aggatatatt
     3001 gtgtaaaata gcattcaaaa tggtttcgtt aatcatcatg tacttaaagt ttgctcttgc
     3061 aactttactt ttgttgctcc gcaccaactc tgacctaact atttttttat cgattaatgt
     3121 aacaatgtca gagcggtatt tcacaaattt ggacgtttta attgaaagtt cgttcaaaat
     3181 atcgttcagg tcgcacgaga aaaacatgga ttgctgcacg taaatgatgg aaaagaacca
     3241 ggcttgttgt tcagtaacat caaggtaatt cgcaagttga aggctttcgg ttttgatttt
     3301 tgcagtcgtt tcgggattca attcggattc gtaaagctc
//
LOCUS       DAOPYB010000276         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_38631_length_3336_cov_13.017678, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000276 DAOPYB010000000
VERSION     DAOPYB010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38631_length_3336_cov_13.017678"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKH58_12965"
     CDS             <1..585
                     /locus_tag="PKH58_12965"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX89983.1"
                     /translation="DEDVLVMGDMNAYAFEDPIQVFIQAGYKDMHRELHGDSAYSYVY
                     RGEAGYLDHALASPSLANQITFVSAFHINADEPAMFEYSGSNYQGNMYRCSDHDPVVV
                     GISLSSGADIDTLPSGKNVKIYPTIVKYTFTIKDAENFYLQIFSANGLKMYETQISSS
                     VAEFDVRQLGLPAGAYVVRVLGNGTIGRQIILVK"
     gene            664..745
                     /locus_tag="PKH58_12970"
     tRNA            664..745
                     /locus_tag="PKH58_12970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Leu,seq:tag)
     gene            complement(804..1217)
                     /locus_tag="PKH58_12975"
     CDS             complement(804..1217)
                     /locus_tag="PKH58_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HNX89984.1"
                     /translation="MKKLFQLMAAIAIALFVTNCGGKEANTPSAIEKTLYTQLQKGDY
                     QKAAELIIKNLSTEKEMTAEESATLIKSFTEKAKQSTEAKGGVKSFEILEEKISEDGL
                     TATVTTKVVYGDGTDKTETTKYEKKDNDWKLSMGK"
     gene            complement(1250..1867)
                     /locus_tag="PKH58_12980"
     CDS             complement(1250..1867)
                     /locus_tag="PKH58_12980"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNX89985.1"
                     /translation="MIRITRKNVIFTLGLVLLTIIGCTEQTANRQFGNIALADGDLIF
                     RKGIGAKSRAVLNVDTGGIYSHSGIIVRNDSGFRVVHITPGERKHGERYDKIKAEPIE
                     EFWSRDKAQNGAIYRLKNNVPCTQAGRQALRLLRKGILFDHDYELGDTTQMYCTELIW
                     YVYRQAGIDITHGKRSRLNVPLYSGTYIFPSDIYRNKDFQLIYKF"
     gene            complement(2223..2768)
                     /gene="thpR"
                     /locus_tag="PKH58_12985"
     CDS             complement(2223..2768)
                     /gene="thpR"
                     /locus_tag="PKH58_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNX89986.1"
                     /translation="MKLYIGIELPDEVKDTLFNVLVSLKQLDVKGKWKAPEFLHITLE
                     FLGELPDDVLPLLSQVLKTVAGSNKAFKLQLEAMGSFPSWSRAHTLWVGVDGNLKQLN
                     SLWSGLHSELKINGFNLQSSKFIPHITLLSRPQLPLPDPDKLSLVKSLRFKVTELILF
                     ESKAVDGRRVYPHIITEKLNG"
     gene            2813..>3336
                     /locus_tag="PKH58_12990"
     CDS             2813..>3336
                     /locus_tag="PKH58_12990"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HNX89987.1"
                     /translation="MFETEGDALALTFKSEHGNYSDGFRKAFHHETISYNRRKDREFV
                     EIQNPRLSALLSGTPKQVSALIPSAENGLFSRFIFYFMNVRPVWNDVFANSSDQTLDS
                     YFNHLGMQFFDLYKHLEYQSEPIRFCLTSGQQQAFNAYFAQTQNQYLCLYGADYLATV
                     RRLGLITFRMAMILT"
BASE COUNT          899 a          706 c          693 g         1038 t
ORIGIN      
        1 gatgaagatg tgctggtaat gggtgatatg aatgcctatg ccttcgaaga tcccattcag
       61 gtatttattc aggccggtta taaagatatg caccgcgagc ttcatggcga cagtgcctac
      121 agctatgtgt accgtgggga agcaggttat ctggatcatg ccctggcaag tccatcgctt
      181 gccaatcaaa taacgtttgt ctctgctttc catatcaatg ccgacgaacc ggccatgttt
      241 gagtactcgg gttcaaatta tcagggcaat atgtatcgct gttcagatca cgatccggtt
      301 gtagtgggta tttccctgtc gtccggtgcg gatatagata cccttccttc gggtaaaaat
      361 gtgaagattt atcctaccat tgttaaatac acctttacga taaaagatgc tgagaatttt
      421 tatttgcaga tattttcggc taatggtttg aagatgtatg aaacacagat ttcatcttcg
      481 gttgcagagt ttgatgtgcg gcagcttggt ttgccggccg gtgcatacgt tgtacgtgtg
      541 ctgggcaatg ggacgatcgg acgtcagata attctggtga agtaatgaaa tttgcgggtt
      601 ttgtaatgtt tcgttgtgag attacaaaaa aaaatatatc tttgtacccg caaaaaggca
      661 catgcggttg tggcgtaatt ggtagccgcg ccagacttag gatctggtgg cgtaagccgt
      721 gggggttcga gtcccttcag ccgcacgaaa acaaactcct tcaagagaaa tcctgaagga
      781 gtttttttat ttacattccg gacttatttg cccatcgaca atttccagtc attgtccttt
      841 ttctcgtatt ttgtcgtttc ggttttatca gtaccatccc catagacaac tttcgtggta
      901 acggtagcgg tcagtccgtc ttctgagatc ttttcctcca gtatttcaaa acttttcaca
      961 ccaccttttg cttcggtact ttgttttgcc ttttctgtga acgatttaat gagtgtcgcg
     1021 ctttcttcag ccgtcatttc cttttcggta ctgaggtttt tgataatcaa ttcagcagct
     1081 ttctgataat cgcctttttg taactgagtg tacagtgttt tctcgatggc agacggagtg
     1141 ttggcttcct tgccgccaca attggtaaca aacaatgcaa tggcaatagc agccattagt
     1201 tgaaatagtt ttttcatggt gataaatttt aaaattaaac attagttaat cagaatttat
     1261 aaattagttg aaaatcctta ttcctgtaaa tatccgaagg gaatatataa gttcccgaat
     1321 acaagggtac gtttaaccgg ctccgcttgc cgtgtgtaat atcaatacca gcctgccggt
     1381 aaacatacca tatcagctcg gtgcagtaca tctgagtggt atcacccaac tcataatcgt
     1441 ggtcaaacaa gattcccttt cggagaagcc tgagcgcctg acgaccagcc tgagtgcacg
     1501 ggacattgtt cttcagccga tagatggctc cattttgcgc tttatccctg ctccagaatt
     1561 cctcaatcgg ttcagctttg attttgtcgt agcgttcgcc atgttttcgt tcgccgggtg
     1621 tgatgtgtac aaccctgaaa ccagaatcat ttctgacaat aattcccgaa tggctgtaaa
     1681 tgccccctgt atcaacgttt aacacggcgc gtgattttgc tccgatacct ttccggaata
     1741 tcagatcacc atcagctaaa gcgatattcc cgaactgacg gtttgctgtt tgttcggtac
     1801 agccaattat tgtcaataag accaggccaa gcgtaaatat gacatttttt cttgttattc
     1861 taatcattac aacctgtgat aaatgttttc agacagttgt ctggaaagca caaaaaaagg
     1921 caattataaa tgagatagtg atttctttct gtttgatggt ggaaatttat caggcatgta
     1981 tatgattttt acttcaatat ctttgatatt cagttagtac gtcgatgccc gatgcgaagc
     2041 ataattttac aaacatacat attttagttt aaatttccac taaaaaatat ttcaaaaata
     2101 caattagttt taattctggt gtgtgatacg ggcaataatt atggtgtggg ttcctgttgt
     2161 cccgatggtt gtctgtaatt tgacacctgg caagtaaacc gtttgaattt cagaataaat
     2221 agctatccgt tcaatttttc agtaatgata tgcgggtaaa ccctcctgcc gtcaactgct
     2281 ttactttcaa ataatatcag ctcagtaact ttaaaacgga gagatttcac caatgacagt
     2341 ttatcggggt ctggaagcgg taattgtgga cgcgataaaa gggtgatatg ggggataaat
     2401 tttgaacttt gcaagttaaa tccatttatt ttgagttcgg aatgcaaacc cgaccaaagc
     2461 gaattcaact gcttgagatt gccgtcaaca ccaacccaga gcgtatgtgc ccgtgaccat
     2521 gaaggaaagg accccatagc ttcgagctgc aatttaaaag ctttattact gccggcaaca
     2581 gtctttagca cctgcgaaag caaaggaaga acatcatcgg gcaattcacc caaaaactcg
     2641 agtgtaatat gaaggaattc aggagccttc cattttcctt tcacatccaa ctgttttaaa
     2701 gacaccagaa cgttgaaaag tgtatccttc acttcgtcag gtagttctat cccaatgtac
     2761 agcttcataa tatgagtgtt agtgattaat aaacaacaac ggctctggac taatgtttga
     2821 aacggaaggc gacgcgctgg cacttacttt caaatccgaa cacggtaatt attccgatgg
     2881 tttccgcaaa gcgttccacc acgaaacaat ttcttataac cgccgaaaag acagggaatt
     2941 tgttgaaatc caaaatcctc gtttgtctgc ccttttatcg ggtacaccca aacaagtttc
     3001 agcactcatt ccgagtgccg aaaacggctt attcagccgt ttcattttct attttatgaa
     3061 tgttcgtcct gtgtggaatg atgtttttgc aaactcttcc gaccaaacac tcgatagcta
     3121 tttcaatcac cttggaatgc aattctttga cctgtacaag cacttagaat atcaatccga
     3181 accaatacgc ttttgtttga cttcggggca acaacaggca tttaatgcct attttgccca
     3241 gacacaaaac cagtaccttt gtttgtatgg ggctgattac ctcgccaccg ttcggcggct
     3301 cggtcttatt acattccgaa tggctatgat actcac
//
LOCUS       DAOPYB010000277         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_39402_length_3292_cov_14.037380, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000277 DAOPYB010000000
VERSION     DAOPYB010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39402_length_3292_cov_14.037380"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKH58_12995"
     CDS             <1..308
                     /locus_tag="PKH58_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNX89988.1"
                     /translation="IRNLLNQDSQIDSVTLGCTHYPLLYNKIARELPEGVKIVSQGEI
                     VAGSLADYLQRHPEMEEKCSRNASCRYLTTENPGKFSSSASIFLAEEIVAEHVEVSE"
     gene            503..1444
                     /gene="mdh"
                     /locus_tag="PKH58_13000"
     CDS             503..1444
                     /gene="mdh"
                     /locus_tag="PKH58_13000"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNX89989.1"
                     /translation="MSKVTVVGAGNVGATCANVLAFNEVADQVVMLDVKEGVSEGKAL
                     DMMQTAQLLGFDTNIVGCTSDYAQTADSDVVVITSGIPRKPGMTREELIGVNAGIVKS
                     VVDNILKFSPNAILVIVSNPMDTMTYLTLKSTGLPKNRIIGMGGALDSSRFKCYLSKA
                     LNANANEVEGTVIGGHGDTTMIPLTRFANYKGRPVADLLDKDALAKVVADTMVGGATL
                     TALLGTSAWYAPGAAAAFVVESIIHDQKKIVPCCVSLEGEYGQNDICIGVPVVLGKNG
                     VEEIIDYKLNDEEKALFAKSADAVRKTNDVLKELNVL"
     gene            1565..2647
                     /locus_tag="PKH58_13005"
     CDS             1565..2647
                     /locus_tag="PKH58_13005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX89990.1"
                     /translation="MIILSLIVIFGYALLIGSFIRGWISLPEYKVTDVRKGPRVTIIT
                     PCKNELKHLPALFEAVKNQTYREFELILVDDGSSDGSWEFASQFASGFPELKLIQNVE
                     VGKKHAIKMAVLQSDSDLIITLDADCVPPAGWLNAIVHFYQANPSDLIICPVNMNSGG
                     SFFQEFQQFEFASLIASGAGAVAMNMPILCNGANLAFRREVWLKNMDRLHFEVPGGDD
                     IFLLQSVKKRKGIIRFLKSREATVTTQSSDNLRSFLNQRRRWASKKSAYADWQLALTA
                     VVIFMASFILLFNLFMAVLHPQSLLIAGILFLLKLLIDTSMFRVVSEFFGLKRVTLNA
                     FLFSLIYPFYIVFTAIISLFNHKKGW"
     gene            complement(2652..3125)
                     /locus_tag="PKH58_13010"
     CDS             complement(2652..3125)
                     /locus_tag="PKH58_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89991.1"
                     /translation="MKGFIHISLLFLLPGCFLCLFSCENEDQRRIPEASVYMELNLAT
                     SYPTFRYSVNDTLVFTHPRNGHPTDRVGYGGILVYTFVNGTETNYCAFDLCCPNEVNP
                     LIRVYPNDQGQAVCKSCGTVYQLLTGTGLVESGPSRWNLKRYTTHLSGDILYISR"
BASE COUNT          945 a          619 c          722 g         1006 t
ORIGIN      
        1 atatccgcaa cctgttgaat caggacagtc agattgacag tgttacactc ggatgtacgc
       61 attacccgct attgtacaat aaaattgccc gggaacttcc tgaaggagtg aagattgttt
      121 cacagggtga aattgtggcc ggaagtctgg cagattacct gcagcgtcat cccgaaatgg
      181 aagaaaaatg ttcgcggaat gcctcttgcc gttacctgac taccgaaaat cccggaaaat
      241 tttcatcttc agcgtccata tttctggcag aagaaattgt tgctgaacat gtagaagtgt
      301 cagaatgact gaatagaata tcttaattcc tctttaacat aattttcaca acattatggt
      361 aatgattaaa gaaatatttc tttaattgct ggataatttc gtatctttgt acgctcgaat
      421 ttttgaacag ttcatgttca ttaagcgttc tgaaattctg attaacggaa aatattttca
      481 aactaataaa aaataaattg atatgtctaa agtaactgtt gtaggcgccg gaaatgtggg
      541 tgccacttgt gcaaatgtgc ttgcatttaa tgaagtagcc gatcaggttg taatgctcga
      601 tgtaaaagaa ggcgtttcgg aaggtaaagc cctggatatg atgcagactg ctcaactact
      661 tggttttgat actaatatcg tgggttgtac cagtgattat gctcagactg ccgattctga
      721 tgttgttgtt ataacctcag gaattccccg taaaccggga atgacacgcg aagaacttat
      781 cggtgtgaat gccggtatcg taaaaagtgt tgttgataat atcctgaaat tttcaccaaa
      841 tgctatcctg gttattgtga gcaatccgat ggatacaatg acatacctga ccctgaaatc
      901 taccggtctt ccaaaaaacc gtattatcgg tatgggtgga gcgctggata gctcacgttt
      961 caaatgttat ttgagtaagg cactcaatgc caatgccaac gaagtggaag gaactgttat
     1021 tggtggtcac ggtgatacaa ccatgatacc tctgacacgt tttgctaact ataaaggtcg
     1081 tccggttgct gacttactcg ataaagatgc tcttgcaaaa gtggtagccg acacaatggt
     1141 aggtggtgca acgcttaccg cgttactggg aacttcagcc tggtatgctc ccggagctgc
     1201 tgctgcattt gttgttgaaa gcattattca cgatcagaaa aaaatcgttc cttgttgtgt
     1261 ttcactcgaa ggtgaatacg gccaaaacga tatttgtatc ggtgtgcctg tagtacttgg
     1321 taaaaacggt gttgaagaaa tcatcgatta taaattgaac gatgaagaaa aagcactgtt
     1381 tgcaaaaagt gcagatgcag tacgaaaaac caacgacgta ttgaaagaac tgaatgtgtt
     1441 gtaatacact gttcttataa atttatagat cgctgtaagc tctaatgctt acagcgattt
     1501 tttttattca aattgggatt attccgattc ctttgattaa ttttgttatc aatcggatta
     1561 taacatgatc attctgtcac ttatagtaat ttttggatat gcactactga taggcagttt
     1621 catcagaggt tggatatctt tacctgaata taaggtgaca gatgttcgga aagggcctcg
     1681 cgttaccatc attacaccct gcaaaaatga actaaaacat cttccggcac tatttgaagc
     1741 agtaaaaaat cagacttacc gtgagtttga gctaatattg gtagatgatg gttcctccga
     1801 cggcagttgg gagtttgcaa gtcagtttgc ttcaggcttt ccggaattaa aactgataca
     1861 aaatgtggaa gttggaaaaa agcatgccat taaaatggct gttttacagt ctgatagtga
     1921 cctgattatt acgcttgatg ccgattgtgt tccacctgcc gggtggctca atgcaattgt
     1981 acatttttat caggcaaatc cctccgatct gattatttgt ccggtaaata tgaattccgg
     2041 tggaagtttt tttcaggaat ttcagcagtt tgaatttgcc agtctgatag ccagtggtgc
     2101 cggtgcggtt gcgatgaata tgccaatatt gtgtaacggt gcaaatctgg catttcggcg
     2161 tgaagtttgg ctgaagaata tggatcggtt gcatttcgaa gtgccgggtg gcgatgatat
     2221 tttcctgctg caaagtgtaa aaaaacggaa gggaataatt cgttttctga aatcaaggga
     2281 agcaaccgtg acaacacagt cttctgataa tctgagatca tttctcaatc agcggcggcg
     2341 gtgggcgtcc aaaaagagtg cttatgccga ttggcaactt gccttaacgg ccgttgtgat
     2401 atttatggcc agttttattc tattgttcaa tctgtttatg gcggttctgc atcctcaatc
     2461 ccttttgata gctggcattc tattcctgct caaactgctg attgatactt caatgttccg
     2521 tgtagtaagc gaattttttg gattgaaaag agttactctc aatgccttcc tgttttcttt
     2581 gatctatccg ttttatattg tctttacggc aataatctct ttattcaatc ataaaaaagg
     2641 ctggtaattg cttatcgtga tatataaagt atatcgcccg aaaggtgagt ggtgtatcgt
     2701 ttaaggttcc accgcgaagg ccctgattcc accaaaccgg ttccggtgag caactgataa
     2761 accgttccgc acgatttgca tactgcctgt ccctgatcgt ttggatatac tcttattaaa
     2821 ggattcacct cattggggca gcaaagatca aatgcacagt agtttgtctc agtaccgttc
     2881 acaaaagtgt aaaccaaaat gccgccataa cccactctgt ccgtcggatg tccgttgcgc
     2941 ggatgagtaa aaacgagggt atcattcacc gaatagcgga aagtgggata ggaggtagcc
     3001 agatttagtt ccatataaac agaagcctcc ggaatgcgac gctgatcttc attctcgcag
     3061 ctgaatagac acagaaagca gccaggcagc aggaatagta aactaatatg aataaatcct
     3121 ttcaagaata gtcagtttaa tgctttattt caaactttat ttcggtttta tattaaaaac
     3181 gttattgtct gtgatttcgt atcccgttaa acgtctttat ttatcatgaa gatggtatag
     3241 gcaatgaaaa tagtaagcag aacaactccc tcccagcgat cgatcttcaa tt
//
LOCUS       DAOPYB010000278         3267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_39844_length_3267_cov_8.103674, whole genome shotgun sequence.
ACCESSION   DAOPYB010000278 DAOPYB010000000
VERSION     DAOPYB010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39844_length_3267_cov_8.103674"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PKH58_13015"
     CDS             complement(<1..308)
                     /locus_tag="PKH58_13015"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HNX89992.1"
                     /translation="MLQNKDELTALQIFYNQPYRRRELTYSTIKEVLDKLKADKPLMA
                     PMHIWNAYAALENVNDNPRNELTALVSLIRRVTDIDTLITPYDKTVDKNFQDWVWKRH
                     S"
     gene            complement(1599..2492)
                     /locus_tag="PKH58_13020"
                     /pseudo
     CDS             complement(1599..2492)
                     /locus_tag="PKH58_13020"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          834 a          718 c          656 g         1059 t
ORIGIN      
        1 gagtggcgtt tccacaccca atcctgaaag tttttatcca ccgttttgtc gtatggtgtt
       61 atcagtgtgt caatatcagt tacacgccga atgagtgaaa ctaatgctgt cagttcgttg
      121 cggggattgt cattcacatt ttccaatgca gcataagcat tccagatgtg cattggcgcc
      181 atgagtggtt tgtcggcctt cagtttatct aacacctctt ttatagtgct gtaagtaagc
      241 tcacgcctgc gatagggttg attgtagaaa atctgtaagg ctgttagctc atctttattt
      301 tgcagcatcc aacttgtaaa gtcggaaata atctgttgaa tttttgcatc tttatcgtcg
      361 ccccaaccaa caaaaataac ttcgtcggga ttgagggtgt ctatcttttg ttcgtgtgcc
      421 cggcgtactt cttcgatata tttattcaac tcgccatgaa atactttggc tgcattggtt
      481 tgcagttgtt ccacggcttc ctgtatggct tcttcaattt caacaggtgc agcaccagcc
      541 ttctcggctc gtacacgggc ttcggtttct tcctgtacat cggggttata ggcattcaac
      601 agtcctttta ctacctgcga aatagttttt ccacccgaca gttccacgaa acgttgtttc
      661 tctttttcgg taatctgctt gtctaatcgt gtcagtcggt ttgccagtga cgtaaacagt
      721 tcctcctcac gtgctcccac tgtaattgcc tgcaggaggt ctttcagtgg aatgcccggt
      781 ttcttttcca gcggtcggct gtcggtcttc agacttcgtg taacacctat ggcatcaaca
      841 ataacaaaat gatctttcgt ggttttggct gttcttgaaa ctttctgtaa actatctaaa
      901 tcaatagtgc gtgttccacg tcctttcatc tgctcaaaat aattacggct tttcacatcg
      961 cgcataaaca acagacattc taatggtttc acatctgttc cggtggcaat catatccacc
     1021 gtcacagcaa tacgcggata ataatcgttg cggaactgcg ccagtgtgcc tttggggtcg
     1081 tcgctgttgt aggttatttt cttgcagaac ttgttttctt cggcaaactc ctcacgcact
     1141 atctgaataa tatcgtcggc atggctgtcg gttttggcaa atatcagggt cttgggcact
     1201 tcaaacgtac catctgtatc gtacctttca gaaaataagg ttggcaaatg ttcgcggaag
     1261 gtgcggataa cggtgcgtat ctggttaggg tttacaatat ctttgtccaa ttgacgggat
     1321 gaatattctt cgtcttcgtc ctgcaactcc atgcgttttt tccggctgag cctttcgcgc
     1381 atctctatgt attcaccttt ccagagtttg ctcccttcgg ttgttatttt ggtgtcgata
     1441 ataaacacat cataacccac attcacatta tcggccacag ccatttcatg cgaatattcg
     1501 cttaccatat tctgattaaa atatccaatg gtgcgtttgt cgggcgttgc tgtcagacct
     1561 atttcaaatg catcgtaata ctccaacacc tgtttccaca gattataaat actgcgatga
     1621 cattcgtcaa tcacaataaa atcgaaaaac tcaacgggca ttttggggtc gtaagccaca
     1681 ggggcaactt cctttggctg aaagatactt taattcggat tgttttcttc ggcagtttca
     1741 tccagctcag ttcctttcag aatagaatat aatcgctgaa tagtagaaat acacacctga
     1801 ctatcggtag ccacgtatga cgatttcagt cgctgaacac tgtatagctc tgtaaaaagg
     1861 cgattgtcgt cgttgggttt gtacgacata aattcctgtt cggcctgctc tcctaagttt
     1921 ttggtatcta ccagaaataa aattcgtttt gctttggcat atttcagcag tcggtatata
     1981 aatgtaatag ccgtaaatgt ttttcccgat ccggttgcca tctgaatcaa tgcgcggggt
     2041 tgattatcct taaaagactt ttcgagctgg gtaatggcat taatctgaca gtcacgcaag
     2101 ccgttttccg gtagttcggg tagatcctgc aacctgctgc gcaatgattt acctgttttt
     2161 atccattgag ccaatgtttc aggacgatgg aagctgaata cttctctcgc acgcggtttg
     2221 ggatcggtaa agtcggtaaa ccgggttaaa tctccggtgc tgacatacac aaatggtaaa
     2281 gggttgttat tgagatgttt gagctttgca gtagcgtagc cttcagcctg atcctcatgc
     2341 atgcttaact tctgtccttc ttcttcgcgt ttggcttcaa taataccaca aggcttgcca
     2401 tccacaaaca gcacatagtc ggcgggacct gcatcagttg gatattcacg cacagccaca
     2461 ccttttgaaa catgcagatt aatatgtttc atgccctgaa tgagccagcc acatgcttcc
     2521 agttgttggt caatcaggtc gcgtgcaatt tgttcgggat tttgattagg catttattga
     2581 aaatttttta agacctcaaa tttaaaaaac aaatttcaat taatctatct ttatgttaaa
     2641 tatattttca atttctcgca caaatgagaa tattgcgata attagttctt ttaaaataca
     2701 ctttatttag tcagtatacc tgcaaatata ttatcgctaa aagtggataa taatacatat
     2761 agggaatttc tgaaagaaaa atttaagtaa aaagcaactc gggttcaccg tattctccgt
     2821 acagagttct cgggttgagc aagtcgaagc ctgttgaagt ctcgcgctaa atggaaatct
     2881 ccatacataa tgttgttttt cataatattt cagcgcatgg cagtttcatc cgcctttcgc
     2941 ttaaaaaata aatctgatac taacagaata ttccatttct tatcccccta tactttggag
     3001 aggggttagg ggtgaggtca gattccccga gtttagcgta gtctctggca ccccagcaca
     3061 ttgcggttcc aagccgcatt gtacgtaaag aggcaaaata caattgttaa ttctaaactt
     3121 ttatgtattt ttggtccaac gaatgatatc tatctgatta caaataaatt tatacaatgg
     3181 gataccttgc tcaaaccgct gccgacatcg cacaaattat tgaaaacatc gactttgtcg
     3241 caaataagaa tactctcacc ttccatt
//
LOCUS       DAOPYB010000279         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_40073_length_3254_cov_8.743982, whole genome shotgun sequence.
ACCESSION   DAOPYB010000279 DAOPYB010000000
VERSION     DAOPYB010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40073_length_3254_cov_8.743982"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..2738)
                     /locus_tag="PKH58_13025"
     CDS             complement(399..2738)
                     /locus_tag="PKH58_13025"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX89993.1"
                     /translation="MKDLLTGIFIVLSILLFIPKSFSQNNEIGYWLNDENNLPCFNYT
                     GKIPFTITIPNGEKAKLPDDPWFLLGNYQLTLFAHVSGTYELITGQRAWGRMNQGDTP
                     NSGINQASLEIIGQQDKKEKILLLTGMNSLSADPVRCDRVFGCGFARYSYHSDSIAID
                     RVLSVKPSTNPYNGTSAFLLTIKIKNESHKKRKLAYNESITAHYQTMQQQHENAEKQK
                     VKYTNTLSADITNQQIIADIHGVTRDPLLFTSKEDISMYEGFPPSLFLKSLSKDINLF
                     NIPKGDRDELTARCEFILSAGEEKNLELIIGYTFENETSSINQICDELSQKHINSCRE
                     KKTFCSGAAYGNDWLKVLPAFNNENNVPLRQELIWHAYNLEAMATYSEFYKETKIPQG
                     TIYDYDWGMHASARDNFQHALPLVYYNPQLAKSVLRYMLKRTTACGEIRLVEMGNGYA
                     NNSSYFTSDQQLFFFMLLAEYLKVTHDYAFLKEEIEYYPVINQSKASVAEFTKQCFRF
                     LRDEIGTGEHGLVRLMNSDWNDAVFYIVDAPYNKVVFSGESHMNSAMALVVFQKLIPE
                     LKSAENSPELASKKSLLENLSKSMEIYRNSILKAYLADWDGRAFPRRMYFNGNAYGED
                     NMFLEPQGYTLLIDEIAVEKKKNLYQEMKRRVYAGEKLGAREQQMPEFEDESFDKGSR
                     ENGGFWWALNGPVINGIAQWDKTEAMRLLKNMTFTHFAEAFPHYWTSYWSAADNIESS
                     LIPGEGLPDQTYNYSDIPVFCAHPHAWILYCYYTIHQNE"
BASE COUNT          983 a          640 c          661 g          970 t
ORIGIN      
        1 gaatctcctt tcggacggaa tgggtgaaat ctacgaatga taaatttcta ccacagattc
       61 acaaatttca cgaattgaaa attcaccgga ggaaatttct cgaataaaaa ttttgtccac
      121 gaattacacg aattgcacga atgaaaaatc taatccacaa attccacaat taaaattcac
      181 cggaggtaaa tttgacaagt aaaaagtaaa aagtaaaaaa ggtaaaaggg cgactggcga
      241 ccgttaacca tggcaactat gataaccatg acaaccgttt aaacagagag acacagagaa
      301 aaaactggtt caggcatcct gtgtggatac tttaacgaaa cagatagccg ttacaaaaaa
      361 caattcaata attcattttt tctttttgca gtattgtctt attcgttctg atgaattgtg
      421 taataacaat ataaaatcca agcatgagga tgagcacaaa agaccggaat atcggaataa
      481 ttatatgttt gatcaggcaa tccctctccg gggattaacg atgattcaat attgtcggca
      541 gctgaccagt acgatgtcca atagtgagga aatgcttctg caaaatgtgt aaaggtcatg
      601 tttttcagca atcgcatcgc ttccgtttta tcccattgag cgattccatt gattacaggt
      661 ccattcaaag cccaccaaaa acctccgttt tcccttgacc ctttatcgaa cgattcatct
      721 tcgaattctg gcatttgttg ttcccgggca ccgagctttt ctccggcata aactcttctt
      781 ttcatctcct ggtataggtt ttttttcttt tcaacagcta tctcatcgat caggagagta
      841 tacccctgtg gttcgagaaa catgttatct tcaccatacg cattgccatt aaaatacatt
      901 ctccgtggaa aggcccgtcc atcccaatca gcaagatatg ccttaagtat ggaattccgg
      961 tatatttcca tgcttttcga caagttttca agaagagact ttttggatgc cagttcaggt
     1021 gagttttctg ccgattttaa ctccggaatc agcttctgaa aaacgacaag agccattgct
     1081 gaattcatgt gcgattcacc cgagaaaaca actttgttgt aaggtgcatc gacgatataa
     1141 aaaactgcat cattccagtc tgaattcatt aaacgaacca gaccatgttc tccggttcca
     1201 atttcatcgc gcaaaaaacg gaaacactgt ttggtaaatt cagcaaccga tgcttttgat
     1261 tggtttatta ccggataata ttcaatctct tctttcagaa aggcatagtc gtgggttact
     1321 ttgagatatt ctgcaaggag cataaaaaag aagagctgct gatcgctggt gaagtagctg
     1381 gagttattgg catatccatt tcccatttca accagcctga tctctccaca tgcggttgtg
     1441 cgtttgagca tgtaacgcaa tacacttttg gccagttgag gattgtaata gaccaatggc
     1501 aaggcgtgtt gaaaattatc acgggcactg gcatgcattc cccaatcata gtcgtagatg
     1561 gtaccctgcg gaatctttgt ctctttgtag aattcgctgt aagtagccat tgcttccagg
     1621 ttgtatgcgt gccagatgag ttcttgtctc aatggaacat tgttttcatt gttgaatgca
     1681 ggcagtactt ttaaccaatc gttgccataa gcggctcccg aacagaaggt tttcttctcg
     1741 cggcaggaat tgatatgctt ttggcttaat tcatcgcaaa tttggtttat ggatgacgtt
     1801 tcattttcaa aggtataacc aataatgagt tcgagattct tttcttcacc ggccgataaa
     1861 ataaattcac aacgcgcagt gagttcatcc cggtcacctt tgggaatatt gaagaggtta
     1921 atgtctttcg acagcgattt cagaaaaaga gatggaggaa aaccttcata cattgaaata
     1981 tcttctttgg atgtaaacag aagcggatca cgggttactc catgaatatc ggcaatgatt
     2041 tgttgattgg ttatatcggc cgataaggta tttgtatatt ttactttctg cttttcagca
     2101 ttctcatgct gttgttgcat cgtttgatag tgagctgtta tcgattcgtt gtaggccagt
     2161 tttcgttttt tatggctttc atttttaatt tttattgtaa gaagaaaggc cgaagttccg
     2221 ttatatggat tggttgaagg ttttaccgaa agtaccctgt cgatggcaat gctgtcggag
     2281 tggtaggaat atcgggcaaa accacatccg aaaaccctat cgcaacggac cggatcggct
     2341 gacaatgaat tcatcccggt taacagcagg atcttttctt ttttatcctg ttgcccgatg
     2401 atttcgagag aagcttgatt gattcccgaa ttgggagtat caccctggtt cattcgtccc
     2461 caggcacgtt gtccggttat aagttcatac gtgccgctta catgagcaaa aagagttaac
     2521 tgatagtttc cgagcaggaa ccagggatca tcgggtaatt ttgctttttc tccattcgga
     2581 atggttatcg taaacggaat tttaccggta tagttaaaac atggcagatt gttctcgtca
     2641 ttgagccagt atccaatttc gttgttctgt gaaaatgatt tgggaatgaa cagaagaatc
     2701 gataaaacga tgaaaatgcc agttagtaag tctttcataa taaagctgtt ttataaaagt
     2761 aacttttttt aaaagtttgt ttgaagataa aaaaaagatg taattgtttt tatcaactca
     2821 aaacaatgtg atgattatcg attcagcgat tgtttcattc acccatgatc ccgggaagat
     2881 ttttgcacag gaatgaaaat caccggaggt aaaagaaaaa ttttcatcac gaaattgaaa
     2941 attaaccgga ggtaatttca caaattaaaa atcaccggag gtaaatttga caagtaaaaa
     3001 gtaaaaaagg taaaagggcg actggcgacc gttaaccatg acaactatga taaccatgac
     3061 aaccgttcaa acagaggcac acagagaaag taaaaagctt gcctgccggt aggcaggtaa
     3121 aaagtaaaaa ggaagcgcat ctaacatccc cgcctgcgct aaagctatgg cgggcaggca
     3181 ccaatccagc atccagtatc cagcatctag tatccagcat ccagattatc cgcgtttaat
     3241 cttttttacc acaa
//
LOCUS       DAOPYB010000280         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_40200_length_3248_cov_8.995929, whole genome shotgun sequence.
ACCESSION   DAOPYB010000280 DAOPYB010000000
VERSION     DAOPYB010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40200_length_3248_cov_8.995929"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1633)
                     /locus_tag="PKH58_13030"
     CDS             complement(17..1633)
                     /locus_tag="PKH58_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX89994.1"
                     /translation="MKTKKLLFLLFITVFTFSVSAAVPTRKGWWKFDDSADMLKATIG
                     NSLTTYGTTPHESVAGPVDGNLAIMDPIGHALVMDHGIGANGGGSLTNEYSLQIDFMV
                     PAPIPGYVAFFQTGPMNDADGDLFVKASGVIGLGDLGWSTKVIVADTWYRMIVSAKCG
                     EYYRIYINGELYLEKLGIAVDSRHALKSLPHIFGDDDGEDPTIHCAELAVWDVALTED
                     QAIELGNASQPMFPQPKGLWKFDDPNNLMLATIGNDLVPEATITHTSALGPAEGNGAI
                     VDGFYQSLVMNHGIEANGGGSLVNVYSMQIDFKAIGTSPSYYSFFQTGATNSGDGDLF
                     IKWSAGTGVVGLGALGWSSNTVVPETWYRLIVTSSGETYKVYINGELWVEKTGILTDS
                     RHALQIANLPHIFADDDGEEGVMHCSELAVWDIVLTPEQVAQLGNATGEGTGLRQPIS
                     VNKSLLGQNYPNPFSVRTTFPYTIQESGNVIFKVLDLTGKQLNVISEGNKTPGNYKFE
                     LNSENLVNGIYYLQMISNNQTSVRKMVVSK"
     gene            complement(1683..3209)
                     /locus_tag="PKH58_13035"
     CDS             complement(1683..3209)
                     /locus_tag="PKH58_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX89995.1"
                     /translation="MKKLNRFFLIGSTLLVFILPGCNQFEDINTNPDATTTVSASLLC
                     TNVILRTTRFGSEAKSYISQNALPKYIGYANEGQMDAQYNRIGSSSFGAMTILPNIEQ
                     MVAAAKGGVLEDSYKGVAKFARAYMFYYLTMEMGDIPYSESGLGETGVFKPKYDTQEE
                     VLKGILDELKEADQYFANGRTFTGDPTPYAGNPDKWRRATNSFALKILMTLSKKEGVA
                     SLNVKNRFAEIVAGGYLLEANTGYFGLNYSTQNKHPLSGTSNLFTSRTIISSLLMDNL
                     KKLNDRRLFYYADPAASKISAGKSQSDTAAYVGVDVSIDYAEMNAGHSASKYSLLNKR
                     YLTEAACEPRMLLTYGEQQLILAEARLRGWITSGNAEDFYKQGVKASLAVVMATKSTY
                     AHGKAIDQNYIDNYFTGEAAFKTTLDDQLKQIWMQRYILSFMQDAEYSYFEYRRNKYP
                     EFPINPATSLNENNVNGIPMRWLYPNSETDYNRENLIIALDRQYGGYDEINKIMWLLK
                     "
BASE COUNT          997 a          674 c          521 g         1056 t
ORIGIN      
        1 caacacaaat taacttttac ttagaaacca ccattttacg tacacttgtt tgattattag
       61 aaatcatttg caaataatat attccattca ctaagttttc agaatttaac tcgaatttat
      121 aattaccagg ggttttattg ccttctgaaa ttacatttag ttgttttcct gttaaatcaa
      181 gaactttgaa tattacattg cctgactctt gtatcgtgta tgggaaagtt gttcttacag
      241 aaaatggatt aggatagttt tgacctaata aacttttatt tacagatatc ggttgacgta
      301 atccggtacc ttctcctgtt gcattaccaa gttgagcaac ctgttcggga gttaatacta
      361 tatcccatac tgctaattca gagcaatgca taacgccttc ttctccatcg tcatctgcga
      421 atatatgtgg taaatttgcg atttgtaagg cgtgtctgct gtcagttaaa atgccggttt
      481 tctctaccca caattcacca tttatgtaaa ctttgtatgt ttcacctgaa gaagtgacaa
      541 tcaaacgata ccaagtttct ggaacaaccg tattgctcga ccaaccaagg gcacctaagc
      601 caacaacacc tgtaccagcc gaccatttaa taaacaaatc accatcgcct gagttagtag
      661 cacctgtttg aaagaatgaa taataactgg gagaagttcc aattgcttta aaatcaattt
      721 gcattgaata cacatttacc aacgaaccac ctccatttgc ttcaattcca tgattcatca
      781 ccaatgattg gtaaaatcca tcaacgatag ctccattacc ttctgccgga cctaaagctg
      841 atgtgtgagt gatagtggct tcaggaacaa gatcatttcc aatagtagcc agcattaaat
      901 tatttggatc atcaaatttc caaagccctt ttggttgtgg aaacattggc tgtgatgcat
      961 ttcccaactc gatagcctgg tcctcagtca atgccacatc ccaaactgca agttcagcac
     1021 aatgaatagt cggatcttca ccatcatcgt caccaaaaat atgaggtaga gatttcaatg
     1081 catgacgact atctacagca attcctaatt tttcaagata taactctcca ttgatgtata
     1141 tcctgtagta ttcaccacat ttagccgaca cgatcatacg ataccatgtg tctgcaacaa
     1201 taacttttgt tgaccagcct aaatcgccta atccaattac accactggct ttaacaaaca
     1261 aatccccgtc agcatcattc ataggacctg tttgaaaaaa cgcaacataa cctggaattg
     1321 gagccggaac cataaaatca atttgaagtg aatactcatt tgtcagagag ccaccaccat
     1381 ttgctccaat tccatgatcc ataactaatg catgtccgat tgggtccatg atcgctaaat
     1441 ttccatctac aggacctgcc actgattcat gaggagtagt accataagta gttagtgaat
     1501 ttccaatagt tgctttaagc atatcagcac tgtcatcgaa tttccaccaa cctttacggg
     1561 ttggcacagc agctgacaca gagaatgtaa agacagtgat aaacagcaaa aaaagtaatt
     1621 tcttcgtttt catgttattt tattttaagt gttaaaaaaa tatggaaatt tctaaacgta
     1681 ttctacttaa gtaaccacat gattttatta atttcatcgt aaccaccata ttgtctatct
     1741 aatgcaatta taagattttc ccgattatag tctgtttcag aatttggata aagccaacgc
     1801 attggaatac catttacatt attttcattc agacttgttg ccggattaat aggaaattca
     1861 ggatatttat ttcgacgata ttcaaaataa ctgtactcag catcttgcat aaaacttagg
     1921 atataacgtt gcatccaaat ttgtttcaac tgatcatcca gtgtagtttt gaaagctgct
     1981 tcgccggtga aatagttatc aatataattc tgatcaattg ctttaccgtg agcatatgtg
     2041 gattttgttg ccataacaac agctaaagat gctttaacac cttgcttata aaaatcttca
     2101 gcattcccgg aggttatcca accacgtaat ctggcttctg ccagtattaa ttgttgttct
     2161 ccataggtta gtaacatacg aggttcacat gctgcctcag tcagatagcg tttgttgagc
     2221 aatgaatact tgcttgcact atgtccggca ttcatctcag cataatcaat cgacacatca
     2281 acacccacgt aagcagctgt atcggattgt gatttacctg cagatatttt tgaagccgca
     2341 ggatcagcat agtaaaataa tcttctatcg ttcagtttct tcaaattatc catgagtaag
     2401 gaactgataa tagttctgct ggtaaataaa ttacttgttc ctgataatgg atgtttgttt
     2461 tgagtagaat aattaagccc aaagtatccg gtatttgctt ccaacaaata acctcccgca
     2521 acaatctcag caaaacggtt ttttacgttt aaagatgcaa caccttcttt tttgctcaga
     2581 gtcatcaata tcttaagagc aaaagaattt gtagctctgc gccatttatc cggattaccc
     2641 gcgtatggtg tgggatcacc tgtaaatgta cgtccattag caaaatactg atcagcttct
     2701 ttcagttcat caagaatacc tttaagaact tcttcctgag tgtcatattt gggtttaaat
     2761 acccctgttt ctcccaaacc actttcacta tatggaatat ctcccatctc cattgtcaaa
     2821 tagtagaaca tataagcacg agcaaatttg gctactcctt tgtatgaatc ttccaaaact
     2881 cccccttttg ctgcagctac catttgttct atattgggaa gaatagtcat agctccaaaa
     2941 ctgctactgc cgattctatt gtattgtgca tccatttgac cttcattggc gtaacctatg
     3001 tattttggca aagcattttg actgatataa cttttagctt cactaccaaa tcttgtagtt
     3061 ctaagaatta catttgtaca tagaagtgat gcgctcacag ttgttgttgc atctggattt
     3121 gtgttaatat cttcaaactg attgcagcct ggcaatatga aaaccagaag agtgcttcca
     3181 attaaaaaaa atctatttag ttttttcatt tgtaattgta ttaaatttta aactattaat
     3241 tatatgtt
//
LOCUS       DAOPYB010000281         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_40387_length_3238_cov_10.446434, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000281 DAOPYB010000000
VERSION     DAOPYB010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40387_length_3238_cov_10.446434"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..1071
                     /locus_tag="PKH58_13040"
                     /pseudo
     CDS             467..1071
                     /locus_tag="PKH58_13040"
                     /inference="COORDINATES: protein motif:HMM:NF024374.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase C-terminal
                     domain-containing protein"
     gene            1194..3134
                     /locus_tag="PKH58_13045"
     CDS             1194..3134
                     /locus_tag="PKH58_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX89996.1"
                     /translation="MGQKLTNTLGQKLVQIPAGNFYMGSLGKGENYDEKPIHKVVISK
                     PFYMSTTEVTNKQYEEFDNNHRKYREYPGMSINDDDPVTYVSYYDAVAFCEWLSKKEN
                     KNYRLPTESEWEYACRAGSYFDYSMGSKLPPEQQKSQTTSRNIPRINLLVAQFEPNLF
                     GLYDMHGNVEEWCSDWYGAYPDIQLTDPVGPVDGMYRVTRGGSFTTPLQYLRSANRMA
                     MLPDNKHWLTGFRIVQAEPLSSNAYKLNEEVPVYARDVTSNQSKWDKKDKRAIFNSPI
                     PYVLQPDCDVFVPFYEHNHCPTITWCDNGDLIAAWFSTDIENGREMVILASRLRNGQT
                     NWDKPSLFFKVPDRNMTGSSLFNTSDGRIIFINGVGSDGDWKNLTMVERSSYDNGSTW
                     TTPRTISSSYDIRNQVITGMIQTKKGALIQLADATPNGEGGTAIHISNDKGKTWTDPA
                     VDRNSRFIEGGTGGNIAGIHATVVELNNGNLMAFGRGNAIEKEGEQRMPMSISKDMGK
                     SWTYYASEFPSIGSGQRAVILRLNEGPILFVSFTNKGLLEKTEQPGMVFINSKGEETT
                     GYGLFAALSFDEGKTWSKKKLLTDGEEKLLNGGAWTGLFIMDKNHAEHRGYLAITQSP
                     DNMIHLLSSNLYYSFNLKWLLE"
BASE COUNT         1120 a          528 c          702 g          888 t
ORIGIN      
        1 atttgtttct ttcaattgtt agaaataatt catttctgga aacttgacac aaaaaagtaa
       61 gaaaaaaaat caatccgcca gctggcggac gcttcactcg aaaaactagc attcggtcgg
      121 ctaaaaccgt ccaaactccc ggcagcggaa tacgccgcca ctcaaacagg acagtttttt
      181 aacgccaccc tcacttgttt ttcggctcac cggacgaggt cgctcgtaaa cgagcctcta
      241 ttttatacat aaaattaaga aattatatga attgactttt ttgtcatgta ctaaaatctt
      301 tgataaataa atcttgcaga aaaatatgtt attaaagaca gaaaaaacaa atcaacagtc
      361 gctttccttt tcactggaaa gcacattaga caataaacac acgttgtttt tgcctaaaca
      421 acaataaaat aatacttaaa tgggcttttt aagactcgac taattaatga ttgcagccga
      481 tgagttgaaa ggcagcgatg gtttccgata cgatttagtg gatgtaacac gtcaagtact
      541 ggcaaactat gcagataccc ttcagcgtaa tttctcgaaa gattatgctg agttaaatta
      601 caaaggatta aaagaaagac caaccgtttt cttaagatca tttcagatat ggatgatttg
      661 tcgggtaccc gtaaagaatg gatgttagga aactgggtaa atgatgctat gaactgtgca
      721 gcaactgaag aggaagtgcc acaatttata aaaaatgcca agaaccttat aacaacatgg
      781 ggaaatgaga atagtgatat ccttgattat tcctggagac tttggtcggg aatgttggat
      841 gactactacc gaccaagatg ggaattgttc tttaaggtaa ttgatgaaag ctggaaaaag
      901 aacaatatcc ccagtattag aggagtagag caacagctta aaggctggga atgggaaggg
      961 attaacagtc ccaaactcta tccaaccgag cccaagggag atgaaattga aatagcgacc
     1021 cgactataca aaaaatatat tgtggaaata aaggcaacat ttcaggatta accgaaaaga
     1081 aagaaaaaac ttattaattt agataataaa agtaaaaaat gagttggttg aataaaaaaa
     1141 gcttcgcaat atttttgttc agtttgtttc tggtacaagg agtacagtct caggtggggc
     1201 aaaaattaac aaacactctg ggacaaaagt tagtacagat tccggcagga aatttttata
     1261 tgggatcatt agggaaaggg gaaaattatg atgaaaagcc tattcataaa gttgtaattt
     1321 caaaaccatt ttatatgagt acaactgaag ttacaaataa acaatatgag gagtttgata
     1381 acaatcaccg gaaatatcgc gaatatccgg gaatgtcaat aaatgatgat gatccggtaa
     1441 cttatgttag ctattatgat gcagtagctt tctgtgaatg gttaagtaaa aaagaaaata
     1501 aaaactatcg ccttcctacc gaatcggagt gggagtacgc gtgtcgtgcc ggatcttatt
     1561 tcgattattc gatggggagt aagctgcctc ctgaacaaca gaaatcacaa acaacatcac
     1621 gcaatattcc acggataaat cttttggtgg ctcagtttga accgaattta ttcggattat
     1681 atgatatgca tggcaatgta gaagaatggt gttctgattg gtatggagcg tatccagata
     1741 tccagttgac tgatcctgtc ggtccagttg atggcatgta tcgggttaca cgaggaggca
     1801 gttttacaac tccgcttcaa tacctgagat cagcaaaccg catggctatg cttccggata
     1861 ataagcattg gcttactggc ttcagaattg tacaagctga gccactaagt tccaatgctt
     1921 ataaattgaa tgaagaagtg cctgtatatg cacgagatgt gacaagtaat cagagtaaat
     1981 gggataagaa ggacaaacgg gctattttta attctccaat accttatgtt ttgcaaccag
     2041 actgtgatgt ttttgtgcca ttttatgaac acaatcattg tccgacaatt acttggtgcg
     2101 acaatggcga tttaattgct gcatggttct caacggatat tgaaaacgga cgtgagatgg
     2161 taattttggc aagtcgtctg agaaatggac aaacaaactg ggataaacca tccttatttt
     2221 tcaaggtgcc agatcgaaat atgacaggtt cgtcattatt caacacaagt gatggaagga
     2281 ttatttttat aaatggtgtc gggagcgatg gtgattggaa aaacctgaca atggtcgaac
     2341 gcagtagtta tgataatggt tcaacatgga caactcccag aacaatttca tcttcatatg
     2401 atataagaaa tcaggtgatt acaggaatga ttcaaacaaa aaaaggagca ttgatacaac
     2461 ttgctgatgc aacaccaaat ggagaaggtg gcacagcaat acatattagt aacgacaaag
     2521 gaaaaacgtg gacagatccc gcagtggatc gtaattcacg ttttatagaa ggagggaccg
     2581 gcggaaatat tgccggaatt catgcaacag ttgttgaatt gaataatgga aatcttatgg
     2641 catttggcag gggaaatgca attgaaaaag aaggtgagca acgtatgcca atgagtattt
     2701 cgaaggatat gggtaagtca tggacatatt atgcgtcaga atttccttcg ataggtagtg
     2761 gacagagagc tgtaattctt cggttaaatg aaggacctat actttttgta tcttttacta
     2821 acaaaggctt acttgaaaag acagagcaac ccgggatggt ttttataaac agtaaaggag
     2881 aagaaacgac gggttacgga ctatttgctg ctttgtcgtt cgatgaagga aaaacatggt
     2941 caaagaaaaa actgcttact gatggggagg aaaaactttt aaacggagga gcatggactg
     3001 gcctgtttat tatggataag aatcatgctg agcatagggg ttatctggca attacccaat
     3061 ctccggataa tatgattcac cttctgagta gcaatcttta ttatagtttt aatctcaaat
     3121 ggctgctaga ataaaaatga caatggaatg gtacccttca tctggaatta aacagataat
     3181 aatttattgg tgtccggtat aaataattca agcgagtata tgttattgaa aatccgac
//
LOCUS       DAOPYB010000282         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_40508_length_3231_cov_10.490869, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000282 DAOPYB010000000
VERSION     DAOPYB010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40508_length_3231_cov_10.490869"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKH58_13050"
     CDS             <1..834
                     /locus_tag="PKH58_13050"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNX89997.1"
                     /translation="ENPKEYRAYVEKLSEVSGVKISNFADLITALRKRHDFFASIGCK
                     LSDHGIEEFYAEPYTQNEIENIFNKVYGGTNLQHDEIIKFKSAMLYEGALMDWEKGWT
                     QQFHYGAIRNNNSRLFGKLGADTGFDSIGDFTVAKSMSRFLDQLDKNNQLAKTILYNL
                     NPRDNDLITTMIGNFQDGSVAGKIQFGSGWWFLDQKTGMEAQLNSLSNLGLLSRFVGM
                     LTDSRSFLSYPRHEYFRRILCNLIGNDVENGLLPASEIDFLGKMVEDISYFNAKRYFE
                     F"
     gene            909..1949
                     /locus_tag="PKH58_13055"
     CDS             909..1949
                     /locus_tag="PKH58_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNX89998.1"
                     /translation="MRKVVTFGEIMLRLATPGYQRFVQSNSLNATFGGGEANVAVSLA
                     NYGIDTEFVTRLPQNDIADWCVSELRKYNVGTKEIIRGGDRVGIYFLETGAVSRSSKV
                     VYDRANSSIADVKPGMINWHEVLKDADWFHWTGITPALSQGAADACLEAIKVANELGV
                     TVSTDLNYRKNLWKYGKKASEIMPELVAGCDIILGNEEDAEKVFGIKPEGFDAEHTGG
                     EVDAAEFQSVCVQLMNKFPRAKKVIITLRGSINANHNTWGGVLYSNDTLYQSKRYDIT
                     HIVDRVGGGDSFMGGLIYGLLTYTTDDQKALDFAVAASCLKHTIYGDFNLVTVSEVEN
                     LMKGDGSGRVSR"
     gene            2209..2874
                     /locus_tag="PKH58_13060"
     CDS             2209..2874
                     /locus_tag="PKH58_13060"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNX89999.1"
                     /translation="MKFNKIQVLTAMSETGIIPVFYHKDGEVAKSVLKACYDGGIRAF
                     EFTNRGDFAQEVFGGLVKYAQVECPEMIVGIGSIVDAPTAAMYIQLGANFVVGPLFNP
                     EVAKVCNRRLVPYAPGCGSVTEVGFAQEAGCDVCKVFPGDVLGTGFVKGLKAPMPWSM
                     LMVTGGVKPEETNLKGWFDAGVTCVGMGSNLFPAEMIKAGDWAGITKLCSDAITIVKK
                     LKK"
     gene            2901..>3231
                     /locus_tag="PKH58_13065"
     CDS             2901..>3231
                     /locus_tag="PKH58_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX90000.1"
                     /translation="MNKTQKMTNFRWVIVTLLFATITINYLDRQVLSLTWDEFIKPEF
                     HFGDDIYGFITGFFSLFYAACYLFAGRFVDWIGTKKGFLWAIGIWSLGACVHAVCGIA
                     TEAVVGLP"
BASE COUNT          961 a          588 c          745 g          937 t
ORIGIN      
        1 gaaaacccaa aggaatacag agcctatgtg gaaaagcttt cggaagtctc cggagttaaa
       61 atcagtaatt ttgctgattt gattaccgcc ttgcgtaaac gtcatgattt ctttgctagc
      121 attggctgta agctttctga ccatggtatt gaagaatttt atgctgaacc atacactcaa
      181 aatgaaattg aaaacatttt caataaagta tatggaggta caaatttaca gcatgacgag
      241 attatcaaat tcaaatcggc tatgctctac gaaggcgctc tgatggattg ggaaaaaggc
      301 tggacacagc aattccacta cggagccatt cgcaataaca attcgcgttt gttcggaaaa
      361 ctcggagccg ataccggttt tgattcgata ggcgatttta cggttgccaa atccatgagc
      421 cgttttctgg atcaacttga taaaaacaat cagctggcga aaacaattct ctataacctc
      481 aatccgcgag acaatgatct gattacgacc atgataggaa attttcagga tggttcagtt
      541 gcagggaaaa ttcaatttgg gtccggatgg tggttcctcg atcagaaaac cggtatggaa
      601 gctcaattga attctttatc aaacctggga ttattaagtc gttttgtagg tatgcttacc
      661 gattcgcgca gtttcctttc ctatccgcgc cacgagtatt ttcgccgcat cctttgcaac
      721 ctgattggca atgatgttga aaacggactt ttgccggctt cagagataga ctttcttggg
      781 aaaatggtag aagatatttc ttacttcaat gcaaagagat atttcgaatt ctaaagaaaa
      841 taataaatag taaattgctt aataagataa ttagaataat aaaaggataa tataaataag
      901 aaactgaaat gagaaaagta gtaacattcg gagaaattat gttgcggtta gcaactccgg
      961 gatatcagcg gttcgtacaa tccaacagtc tgaacgcaac atttggcgga ggagaggcca
     1021 atgtagctgt ctcactggcc aattatggaa tagatactga atttgttacg cgtttgccgc
     1081 aaaatgatat agcagactgg tgtgtttcgg aactgagaaa atacaacgta ggaaccaaag
     1141 aaataatcag aggtggcgat agggtaggta tttattttct cgaaaccgga gctgtttcgc
     1201 gtagtagtaa agtggtatat gaccgtgcaa actcatccat tgcagatgta aaacccggca
     1261 tgatcaactg gcatgaagtt cttaaagatg cagactggtt tcactggaca ggaataactc
     1321 cggcgctttc gcagggagca gctgatgctt gtctggaagc aattaaagtg gccaacgaac
     1381 tgggcgtaac tgtttcaact gatcttaatt accggaaaaa tctctggaaa tacgggaaaa
     1441 aagcatccga aataatgcca gaacttgttg ccggttgcga tatcatatta ggtaacgagg
     1501 aagatgccga gaaagttttc ggaattaaac ccgaaggttt tgatgcagag cataccggtg
     1561 gcgaggtaga tgccgcagaa tttcagtctg tatgcgttca gctgatgaat aaattcccaa
     1621 gagccaaaaa agtaattatt acccttcgtg gttctatcaa tgccaatcat aacacctggg
     1681 gcggagtgct ttacagtaat gacactctgt atcagtccaa acgttatgat attacccata
     1741 ttgttgaccg cgttggtggc ggcgattcgt ttatgggtgg ccttatttat ggactgctga
     1801 cttatactac cgacgatcag aaagcgcttg atttcgctgt tgcggcttct tgtctgaaac
     1861 ataccattta tggtgatttc aatctggtta cagtatcaga agttgaaaat ctgatgaagg
     1921 gtgacggtag cggacgcgtt tccagataaa gagcccctga cccctaaagg ggaaataaat
     1981 ttgtctaacc attaatcaaa gaaataaaat catcactttt actttgggtg attatataag
     2041 atagctatat tatcttcaaa tatttccgtc aagtgggtta gagatttctc aataaattat
     2101 gaaacgagca cgactatata acatttacac acgacttgtc ccgatttatc gggaaggaga
     2161 tgtttatgtt cgggttccat ctgacaatta aaaacaaatt ataaacacat gaaattcaat
     2221 aaaattcagg tactgactgc aatgtcagaa accggtatca ttccggtgtt ttatcataaa
     2281 gacggcgaag ttgccaaaag tgtactaaaa gcttgctatg acggaggtat tcgtgcattt
     2341 gaatttacca accgcggtga ttttgctcag gaagtttttg gcggattagt taaatatgct
     2401 caggtagaat gccctgaaat gatagttgga atcggttcta tcgttgatgc acccacagca
     2461 gctatgtata ttcagttggg agccaatttt gtggttggtc cgctcttcaa tcctgaagtg
     2521 gcgaaagttt gtaaccgtcg attagtgccc tatgcacccg gatgcggttc ggttactgaa
     2581 gtcggctttg cacaggaagc cggttgcgat gtttgcaagg ttttcccggg cgatgtgttg
     2641 ggaactggtt ttgtaaaagg tttgaaagca cccatgccat ggtccatgct gatggttacc
     2701 ggtggcgtaa aaccggaaga aaccaatctg aaaggctggt tcgatgccgg agttacctgt
     2761 gtgggcatgg gatccaatct gtttcctgca gaaatgatca aagctggtga ttgggcggga
     2821 ataacaaaac tttgcagcga tgccattaca attgtgaaga aattgaaaaa ataatattct
     2881 ataacaataa aaattatatc atgaataaaa cacaaaaaat gacgaacttt cgatgggtaa
     2941 ttgtgacttt actgtttgca acaataacca tcaactatct tgaccgtcag gtactttcgc
     3001 ttacgtggga cgaatttatc aaacctgaat ttcattttgg cgatgatatt tacggtttca
     3061 taaccggttt tttttctctt ttttatgctg catgttactt gtttgccgga cgttttgtcg
     3121 attggattgg cactaagaaa ggctttcttt gggctatcgg aatttggtcg ctgggtgctt
     3181 gtgtacacgc tgtttgtgga attgctaccg aagctgtggt aggtctgccc g
//
LOCUS       DAOPYB010000283         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_40569_length_3227_cov_11.295397, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000283 DAOPYB010000000
VERSION     DAOPYB010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40569_length_3227_cov_11.295397"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /gene="cas2"
                     /locus_tag="PKH58_13070"
     CDS             complement(<1..178)
                     /gene="cas2"
                     /locus_tag="PKH58_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009231974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNX90001.1"
                     /translation="MLSKLNQYRIMWVLVFFDLPVVEPKERKVAARFRKDIMADGFAM
                     FQFSIYVRHCASAEN"
     gene            complement(212..1147)
                     /gene="cas1"
                     /locus_tag="PKH58_13075"
     CDS             complement(212..1147)
                     /gene="cas1"
                     /locus_tag="PKH58_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HNX90002.1"
                     /translation="MIKRTLYFANPAYLHLDNKQLIVKLPQVEKNQEMHENIKKTLAA
                     SIPIEDIGVVILDDKQLTITQALMAALLENNVALISCAENHMPSGLFLPLAVNSVQNE
                     RYRTQLEASVPVKKQLWQQTVAAKIRNQAAMLKKYGQDASWLMKWAGDVRSGDPDNYE
                     AQAAAYYWQHIFPETLNFKRSRDGDAPNNLLNYGYAILRAVVARGLVATGLMPTLGIH
                     HENKYNAYCLADDIMEPYRPFVDSVVCEIVYSGIDYTELNKDIKQKLLSIPAMDISID
                     GENSPLMVGLQRTTASLFKCFEGSAKKIIYPELIF"
     gene            complement(1159..1842)
                     /locus_tag="PKH58_13080"
     CDS             complement(1159..1842)
                     /locus_tag="PKH58_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90003.1"
                     /translation="MSAADNKRFKDTPNRWQIWNYSAPAKYFITFCTQNMEMLFGNIV
                     NKTMNLSDAGKCVSDEIQKLPTHHPRIILDENVVMPNHIHLIIELGDYFFDNKIAGQI
                     HEFDRTKKPEIQNPVGQIHEFALRDFDEQEYNKIKNAINDSNGIVNIDMIKQYRKLRR
                     RMLIPMIIGKLKMQTSKQINILNKTPQNKNWQTDYYDHIIRDEQSYWTIKNYIKNNPA
                     RWNEDNIYG"
     gene            complement(2096..2527)
                     /locus_tag="PKH58_13085"
     CDS             complement(2096..2527)
                     /locus_tag="PKH58_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90004.1"
                     /translation="MIFQSFHDKFPEIAEAETRVVLVFENNKFNLPPDDYIFVEMFCN
                     DKKCDCRRVFFDVFSEKRKRLEAVICWGWESRDFYKKWLGINDKEMITELIGPALNVG
                     SQQSELAPALLRLLTESLLTDIEYCNRIKRHYQMFKKKTLF"
     gene            complement(2922..>3227)
                     /locus_tag="PKH58_13090"
     CDS             complement(2922..>3227)
                     /locus_tag="PKH58_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90005.1"
                     /translation="LIESFTRLKFNPDDINLIEKLDFSGVVIFHIGAEPDRIDFLTRV
                     DGLIFEEAFNNRVFLKIGDYEIPILKLEDLITNKLITGRLKDKADIEQLNMIRKRKK"
BASE COUNT          887 a          622 c          571 g         1147 t
ORIGIN      
        1 cattttcggc actggcacaa tgccttacgt atatcgaaaa ctggaacatg gcaaagccat
       61 ctgccataat atccttacga aaacgggccg caactttgcg ctctttaggc tcgacaacag
      121 ggagatcgaa aaatacaagc acccacatga tacggtattg atttaatttg cttaacatgg
      181 ctctgtttgc tgactttgaa gcatcaaccg gtcagaaaat taattccgga taaataattt
      241 tttttgcact accttcaaaa catttgaaca aggatgccgt agttcgctgc aggccaacca
      301 tcagcgggct gttttctccg tcgatactaa tatccatggc cggaatgctc agcagctttt
      361 gtttgatatc cttattcaat tcggtataat caataccact atatacaatt tcgcaaacca
      421 cactgtcgac aaagggacgg taaggttcca tgatgtcgtc ggccaggcaa taggcattgt
      481 acttgttttc gtgatgaatt cccaatgtgg gcattaaacc tgtggccact aaaccacggg
      541 ctaccacagc gcgtaatatc gcataaccat aattcaaaag gttgttgggc gcatcgccgt
      601 cacgtgacct tttaaaattc aaggtttccg ggaaaatatg ctgccagtaa taggcggcgg
      661 cctgtgcctc ataattgtcg gggtcgcctg aacgaacatc gccggcccat ttcatcagcc
      721 acgaagcgtc ctgcccgtat tttttcagca tcgctgcctg gttgcgaatt ttggcggcca
      781 cggtctgttg ccataactgt ttttttactg gtacactggc ttccaattgg gtgcgatagc
      841 gttcgttttg cacactattg acagcaagcg gcaagaacaa tccggagggc atatggtttt
      901 cggcacaact gatcagggcc acattgtttt caagcaaggc cgccatcagc gcctgtgtaa
      961 tggtaagctg cttgtcgtcg agtatcacca cgccgatatc ttcaatgggg atgctggcgg
     1021 ctaaggtttt tttgatgttt tcgtgcattt cctggttttt ttccacctga ggcagtttca
     1081 cgatcagctg tttgttgtca agatgcaggt aagcggggtt tgcgaaatat aatgtacgtt
     1141 ttatcatgac agattttttt atccgtaaat attatcttca ttccaacgtg caggattatt
     1201 tttgatataa tttttgatgg tccaatacga ctgctcgtcg cgtatgatat gatcgtaata
     1261 atcggtttgc caatttttat tttggggcgt tttgttgagt atatttattt gttttgatgt
     1321 ctgcattttt aattttccga tgatcatcgg aatgagcatt cgtctgcgca atttccggta
     1381 ttgtttgatc atgtcgatat tgacaatacc attcgaatca ttaatggcgt ttttaatttt
     1441 attatattcc tgttcgtcaa aatcccgtag ggcaaattca tgaatttgcc ctacgggatt
     1501 ttgaatttct ggtttttttg ttcggtcaaa ttcatgaatt tgacctgcaa ttttattatc
     1561 aaaaaaataa tcccccaatt cgatgattaa atgtatatgg ttgggcatta caacattttc
     1621 gtctagaatt atgcgcggat gatgagttgg taatttttga atttcgtccg aaacgcattt
     1681 tccggcatcc gacaaattca ttgttttgtt cacaatgttt ccgaacaaca tttccatgtt
     1741 ttgtgtacag aacgtaataa aatattttgc aggagccgaa taattccaaa tctgccatct
     1801 gtttggagta tctttgaatc gtttgttatc tgctgctgac acgaattatg atttttttac
     1861 gaattaactg ttagacaaat tcacgaattt gtccgaaaaa tctcttactc actatctttt
     1921 ttcaatattc aaaaaaacaa ttctgattag attgtttttt gaataaatcg tcggacaaat
     1981 tcgtgaattt gtccgactgc tttataaaat tatccgattc atttaataaa acgctacgtg
     2041 ttttcccctg attgtatgtg tggttagtat atctgtgttt tcaaaataca tggttttaaa
     2101 ataatgtttt tttcttaaac atttgataat gtcttttaat tctattgcaa tattcgatat
     2161 ctgtcaataa tgattcggtt aataatctaa gcaaagcagg cgcaagttca gattgttggc
     2221 tgccgacgtt taatgcaggg ccgatcagct ctgtaatcat ttctttatca tttattccaa
     2281 gccacttttt atagaaatct ctgctttccc aaccccaaca aataacagcc tccaatcttt
     2341 tccttttttc tgaaaaaaca tcaaaaaata ctctacggca atcgcatttt ttgtcgttgc
     2401 aaaacatttc cacaaatata taatcgtctg gtggaaggtt aaatttatta ttctcaaaaa
     2461 ccaaaacaac tctggtttcg gcttctgcaa tttcaggaaa cttatcgtga aaagattgaa
     2521 atatcatacc atgtattaat ttttattacc cttaacttaa ataatgttgc ctaatctatc
     2581 gactttcaat tttcaacaaa attctttgat tattatatct tcaattgatt ttacacattt
     2641 gctcaatgcg gaaaactcta ctatattaat tatcggttgt tttatcaatc atttataact
     2701 atcccgggtt gtatttgaat cgtttgttat gtcggacaaa ttcacgaatt tgtccgaaaa
     2761 atatcaaaat tattttcttc tttttgtttt caaataaatc aattctgatt tcattatttt
     2821 ttagaatttc tccgaaaaac tctgatttta ctcaaatttt ttcttctttg tgtatataat
     2881 aatcaattat aaattcattg ttattgtcct ccgggagaaa tttatttctt tcttttcctg
     2941 atcatattga gttgttctat atcagctttg tctttgaggc ggccggtaat taatttgttt
     3001 gttatcaaat cctccaattt caggatggga atttcataat cgcctatttt aagaaaaacc
     3061 ctgttgttaa aggcttcttc gaaaattaat ccatcaacac gggttaaaaa atcaatacgg
     3121 tccggttctg cgcctatatg aaatatcaca acgccgctga agtctaattt ttcaatgagg
     3181 tttatgtcat caggattgaa ttttaatcgt gtaaaacttt ctatcag
//
LOCUS       DAOPYB010000284         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_41204_length_3193_cov_6.948693, whole genome shotgun sequence.
ACCESSION   DAOPYB010000284 DAOPYB010000000
VERSION     DAOPYB010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41204_length_3193_cov_6.948693"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="PKH58_13095"
     CDS             complement(<1..178)
                     /locus_tag="PKH58_13095"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNX90006.1"
                     /translation="MKKLKLKPPLTYYGGKQLMLRNILPIIPEHTIYNEPFFGGGALF
                     FAKEPSKIEFINDIS"
     gene            complement(340..786)
                     /locus_tag="PKH58_13100"
     CDS             complement(340..786)
                     /locus_tag="PKH58_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90007.1"
                     /translation="MSTLADLKIIAAAIKNETTVGANTASKVGALLESFIDNAIILLA
                     SVNSLNNVVFSAPKNISIATGDDYDLTTNDRYINFSAADDHTPVNLMDCMANVGQIHT
                     IINSGSDTIDITPLGGDKINNSTAIRVLGPSTAMSLISFGSTWLII"
     gene            complement(788..1546)
                     /locus_tag="PKH58_13105"
     CDS             complement(788..1546)
                     /locus_tag="PKH58_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90008.1"
                     /translation="MNKLQTNYNGGMALELDDIRWEQDAVREAFFGLISAFGITAATS
                     FKLSGCVVTIAGSNYSCTDGYICLSGEVYKVDAHTAVLGAGEHMSFGVDVSYDAAGLE
                     TFEDTTTHNTYEIRKAKLISGVVGMGGAMSYNAPYLFEILRQQISDVSSWIAPIFASG
                     WSDLGGMNEVSGYRKDGFGMVELKGVITSVTPTGIIFILPAGYRPLFTRDYPCQYGLS
                     MVVITVYPNGEVHVKDPGNTTTDIDLSLITFKTT"
     gene            complement(1548..2051)
                     /locus_tag="PKH58_13110"
     CDS             complement(1548..2051)
                     /locus_tag="PKH58_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90009.1"
                     /translation="MFNVNLNSIITWLLPTFLRGATLIAILKALLHPLQSLHFLLDAF
                     RIQKQYELSITSQVIWLEKMLNTIFDPLNELIYIQDVGAVNQVYMSNKAESPHPRYLY
                     NKAEAAYPTYIYNKSEDIYQFNFTVMVPLAIYNNLLLNNNSGLNQMKANLNKYKMFGT
                     IYIIQSY"
     gene            complement(2044..2925)
                     /locus_tag="PKH58_13115"
     CDS             complement(2044..2925)
                     /locus_tag="PKH58_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90010.1"
                     /translation="MRTVSEIKNEMTAAFMADTSIQAKYDPNSTWTNTTTFDSVFASS
                     SLESILFYIVAVCCYGLEYLFGVHLTEVAAYETKMRVGTKEWWRQLCFSFQLGDSLEF
                     NSSTNTYEYPTIDETAKIIEYVDVRETTNGLTILIAKDDGTGSPEKLDTVDLTERNAF
                     DAYIKKVKIAGIPLSWNSYDADQVKITLVIKYDPLVLDASGQLIGTTTKPVDAAVETY
                     LQTIPFGSGIMNKTQIIDTIQAVEGVVDVYPASADWLEISTSYVPAYTPVSDQNVTAY
                     GGSFALNTLTITYSANV"
     gene            complement(2925..>3193)
                     /locus_tag="PKH58_13120"
     CDS             complement(2925..>3193)
                     /locus_tag="PKH58_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90011.1"
                     /translation="GMIITAIERQNMFDIALIGSGSAENAYAIAYANGKSISEHINEE
                     DQITVSSITNQRIVNFFKVQRPLPATCDITVEGIFDSTFDNTFE"
BASE COUNT          958 a          659 c          580 g          996 t
ORIGIN      
        1 cggagatgtc attgataaac tcaattttac tcggttcctt agcaaaaaac aatgcgccac
       61 ctccaaaaaa tggctcattg taaattgtat gctccggaat tataggtaat atattcctga
      121 gcattagttg tttcccgcca tagtaagtca atggcggttt taattttagt tttttcattg
      181 tatttgttga ttaaattaat aattttgcaa cgtctcttac gaaaacagcc atacgactga
      241 aggtaacacc tccggtgccg tatggctgtt tttaataata agtaagagac gacttttttt
      301 taaaaaaccc ggaggttttt ttattttaat taattcaaat caaattatca accaggttga
      361 accaaaagat attaatgaca tagcagttga tgggcctaac acccttattg ctgtagaatt
      421 gttaatttta tcaccaccaa gtggcgttat atctatcgta tcactaccag aattgataat
      481 tgtatgtatt tgacctacat ttgccatgca atccattaag tttaccggcg tatgatcgtc
      541 tgctgctgaa aaattaatgt acctgtcatt agtcgttaag tcataatcat cacctgtagc
      601 gatactgata tttttaggag cactgaatac aacattattt aagctattaa cacttgcaag
      661 gagtataata gcattatcta taaagctttc taataaagca ccaaccttag aggccgtatt
      721 agctcctaca gtagtttcat tttttattgc agctgcaatt atttttaaat ctgctaatgt
      781 tgacatttta agtagtttta aaggttatta gtgataaatc aatatcagta gtcgtattac
      841 cgggatcttt tacgtgaacc tctccgttag gataaactgt aattacaacc attgataaac
      901 cgtattgaca aggataatct ctcgtaaaaa gtggcctata acctgcgggt agtataaaaa
      961 taataccggt tggcgtgacc gacgtaataa cacctttaag ttctaccatc ccaaaaccat
     1021 ctttacgata tcctgaaact tcattcatcc cacccaaatc ggaccatcca gaagcaaaaa
     1081 ttggcgcaat ccaagatgag acatcagata tctgttgtct caatatttca aaaagatatg
     1141 gtgcattata cgacatggca ccacccatac ccacaacgcc actgatcagc ttagcctttc
     1201 tgatctcata ggtattgtga gtagtagtgt cctcaaatgt ttcaagacca gctgcatcat
     1261 aactcacatc aacaccaaaa gacatatgtt cacctgcccc caaaacagca gtgtgtgcat
     1321 caactttata aacctcacct gataggcata tataaccgtc agtacaactg taattgcttc
     1381 cagcaatggt cactacacaa cctgacaatt tgaatgatgt agctgctgtg atcccaaatg
     1441 cggaaataag tccgaaaaat gcttcacgaa ctgcatcctg ttcccagcgg atgtcatcaa
     1501 gttccagggc catgccccca ttataatttg tttgtaattt attcatatta gtagctttga
     1561 atgatgtaaa tagttccgaa cattttgtat ttattaagat tagccttcat ttggtttaat
     1621 cctgagttat tatttagaag taaattatta tatattgcca gtggcaccat caccgtaaaa
     1681 ttgaactgat agatgtcttc tgacttgtta taaatgtatg ttgggtaagc agcttcagct
     1741 ttattgtaaa gatatcgtgg atgaggactc tcagccttat ttgacatata tacctggttg
     1801 acggccccaa cgtcctgaat gtagatcaac tcattcagcg gatcaaaaat ggtgttcagc
     1861 attttttcca accagatcac ctggctggtg atgctcagtt catattgctt ttgaatcctg
     1921 aaggcatcaa gcaggaaatg gagtgactgt aatgggtgta ataatgcctt tagtatggca
     1981 attaaggtcg ctcctctcag gaatgtaggc aataaccagg tgataatcga attcaaattt
     2041 acattaaaca ttagctgagt aggttatagt taatgtattg agcgcaaaag aacctccata
     2101 ggctgttaca ttctgatctg ataccggtgt ataagccgga acataactgg tactgatctc
     2161 caaccaatct gctgaagccg gataaacatc cacaacacct tcaacagcct gaatagtatc
     2221 aatgatctgt gttttattca tgatcccaga gccaaacggt atggtttgca gataagtctc
     2281 gacagcagca tcaacaggtt tggtggtagt gcctatcaac tgccctgatg catcaagaac
     2341 cagaggatca tattttataa ccaaggtgat cttgacctgg tcagcatcat aactattcca
     2401 cgaaagagga ataccggcaa tcttgacttt cttaatgtag gcatcaaatg cattacgttc
     2461 tgtcaggtca acggtgtcca atttctccgg gctaccggtg ccatcatctt ttgcgatcag
     2521 aatagttaag ccgttggtgg tttccctgac atccacatat tctatgatct tggctgtttc
     2581 atcaatagtg ggatattcat aggtattggt tgacgaattg aactcaaggc tgtcacccaa
     2641 ctggaagctg aagcataact gtcgccacca ttcctttgtg cctacccgca ttttggtctc
     2701 ataggctgct acttcagtaa ggtgaacgcc aaataagtac tctaagccat aacagcaaac
     2761 cgcaacgatg taaaataata ttgattccag gctggaactg gcaaacacac tatcaaatgt
     2821 cgttgtgttt gtccaggtac tattaggatc atactttgcc tgtatactgg tatcagccat
     2881 aaaggctgct gtcatttcat tttttatctc tgatactgtt ctcattattc aaatgtatta
     2941 tcaaatgtgg agtcaaaaat cccttcgact gtaatgtcgc aagtcgccgg aagcggcctt
     3001 tgcactttaa aaaaattaac gatgcgttga ttagtaatgc tgctcacagt aatttgatct
     3061 tcctcgttta tatgttctga aatgcttttg ccattggcat aagcaatagc gtatgcattc
     3121 tcggcgctgc cagaaccaat cagcgctata tcaaacatat tctgccgttc tattgccgta
     3181 attatcatac ctc
//
LOCUS       DAOPYB010000285         3139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_42231_length_3139_cov_10.020428, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000285 DAOPYB010000000
VERSION     DAOPYB010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42231_length_3139_cov_10.020428"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="PKH58_13125"
     CDS             complement(<1..1648)
                     /locus_tag="PKH58_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90012.1"
                     /translation="MGRYGLPYMAPGQPTSIDWDFVELTPDANSQLVVLPPSVDNSQK
                     TWFPYIYNQGSSGSCVHVSEVFYMFTYELNRKFNREAGTDLTKTNLFHPLYSYNYLNN
                     GDGSSFTGRRDGFKIVRDCGVPEWIIYDDSALLTNSTKYKYWMTGYDKYNAGMCNTIS
                     NTMFQFPFTYDNTFLNNIKHWLNDHCNASASGGLASIGVFTIPWVISQVQSGTYATQN
                     YIKQLGVNGIGSGHQMTICGYDEDVTLDWGGGGTAGNPQPDGQFRNDIDNNHDGVIDI
                     RDYEIGAFKVANSWGENWDNGNQGFIWMPYCLFYPGNIGFNGWEAESCDVFGETGEIP
                     QPAINLKVNMEHDHRDHIKCSAGYAPAANNNNPLAWDDLFHLTYRGGFNPMRGCYTGP
                     IDFGINVSQFYDPDSIGKLFFQVKENNNSGESFHGKINSLSLLDSRWGEEFELPCKHS
                     NFTIPNNSTTTLSIEYHLLPHESPLSTDLTLNSNRVSRFTTTVPNGKTLSVGTNANID
                     MYNSEIHIEEGGNLVLSNYDTIIAKRGTCKLVIDGNISVGG"
     gene            complement(1751..3019)
                     /locus_tag="PKH58_13130"
     CDS             complement(1751..3019)
                     /locus_tag="PKH58_13130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90013.1"
                     /translation="MKHKILLFVLASLMVALNGEAQDFWETLPFPDTLDISCVAVNDQ
                     GHIFVGTGTTGPCVSDGVYRSTDRGQTWQHVLNTGTISILAIGIDKTGHVYVSSQGAT
                     TFWKTTDNGQTWQSIPFNSMCHITKFYSFGNDSLLIGCSQTLGALLLRTYDGGITFDT
                     LFQTYNHVSESVRDIAIEPNGDIYIGLQGWSLGAGGVVRSVDNGATWQYQGLQGYQVI
                     NLESNAQGDIFIGAGDDGTFAIYHDNPDEIKFLFWPSNYGMVLNSSGYLYSNSDWPDG
                     TLRSMDNGMTFEWVSNGTLNGPNGELTIDDNDFLYGIFISALPNLYRSKEPTYTGLQS
                     KELAGKTHLMIYPIPAKDILNCRFLQNSIVGEKCKLSIVDINNRILYQEEVLIDSDSY
                     KLNITHLPPGLYCINFTSNQEIVTAKFVKL"
BASE COUNT          910 a          683 c          593 g          953 t
ORIGIN      
        1 taccgccaac cgagatattc ccgtcaatga ccagtttgca ggttccccgc ttggcaatga
       61 tggtatcata atttgacaga acaaggttac cgccttcttc aatatgtatt tcgctgttat
      121 acatatcgat gtttgcattt gtcccaacgg ataatgtttt gccattagga accgttgtcg
      181 taaacctgga aacccggtta ctgtttaagg ttaaatctgt cgatagaggc gattcatgag
      241 gaagcaggtg gtattcaatc gagagggtgg tcgttgagtt attaggtata gtaaaattag
      301 aatgcttgca gggcagctca aattcctcgc cccatcttga atccagtaat gaaagtgaat
      361 ttattttccc atgaaatgat tcaccactgt tgttgttctc tttaacctga aagaataatt
      421 ttcctattga atccggatca taaaactggc ttacattaat cccaaaatca atagggcctg
      481 tataacaccc ccgcattggg ttaaaaccgc cacggtaagt taaatggaac aaatcgtccc
      541 atgctaacgg gttgttatta ttggcagcag gtgcataacc ggcactacat ttaatatggt
      601 ctctgtgatc atgctccata tttaccttca ggttgatggc aggctggggt atttcaccgg
      661 tttcgccaaa tacatcgcaa ctttcagctt cccacccgtt aaagccaata ttaccaggat
      721 aaaaaagaca atagggcatc cagataaacc cctggttccc attatcccaa ttttcacccc
      781 agctatttgc cactttaaaa gcccctatct catagtccct gatgtcaatc actccatcat
      841 ggttgttatc aatatcatta cggaattgtc catcgggctg cggattaccg gcagtaccgc
      901 cgcctcccca atctaatgtt acatcttcat catatccgca aatggtcatc tggtgacccg
      961 aaccaatgcc gtttactccc aactgtttaa tgtaattttg agtcgcataa gtacctgact
     1021 gtacctggct aataacccag ggtattgtaa aaacccctat cgaggcaagc ccgccggaag
     1081 ctgaagcatt gcaatgatca tttagccagt gtttgatatt gttcaggaaa gtattatcgt
     1141 aagtaaacgg aaactggaac atggtattgg aaatggtatt gcacatcccg gcattgtatt
     1201 tgtcgtagcc ggtcatccag tatttgtatt ttgttgaatt ggttaaaagt gcagagtcat
     1261 cgtaaataat ccattcgggc accccgcaat cccttactat tttaaaacca tctcttctgc
     1321 cggtaaaact ggagccatca ccattgttaa gatagttgta tgagtataaa ggatgaaaga
     1381 gattggtttt tgtcaaatca gtaccagctt ctctattaaa tttcctattt aattcatacg
     1441 taaacatata gaagacttca gacacgtgca cgcaactacc acttgaaccc tgattgtaaa
     1501 tgtagggaaa ccaggttttc tgcgaattgt caactgatgg tgggagtact accaattgcg
     1561 aattcgcatc cggtgtcaat tccacaaaat cccaatcaat gcttgtgggc tgacccggag
     1621 ccatgtacgg taatccatac cggcccatta gggtcggggt tcatgttgat aagttgggct
     1681 tttaccgggc agcttattgt caaagcgaag gcagttaaaa taaaatatac ctttttcatg
     1741 tgtaatggat ttaaagttta acaaactttg cagtaactat ttcctggtta ctggtaaaat
     1801 ttatgcagta taaacccgga ggtaagtgtg taatatttaa tttataggaa tctgaatcaa
     1861 tcaaaacttc ctcttgatac aaaatccggt tattgatatc aacgattgac agtttacatt
     1921 tttctccaac tatactattt tgtaaaaacc tgcaattcag tatgtctttt gccggaattg
     1981 gataaatcat gagatgcgtt tttccggcaa gttctttgga ttgtagtccg gtataagttg
     2041 gttcctttga tctgtataaa tttggtaagg cacttataaa aataccatag agaaaatcat
     2101 tgtcatcaat agttaactct ccatttggtc cgtttaatgt accattgcta acccattcaa
     2161 atgtcattcc gttatccatt gatcgaagtg ttccatcagg ccagtcagaa ttggaataca
     2221 aataccctga tgagttcaat accatgccat aattacttgg ccaaaacaaa aacttgattt
     2281 catccggatt gtcgtgataa atggcaaaag taccatcatc tccagcgccg ataaaaatat
     2341 caccctgtgc attgctctca aggttaatta cctggtatcc ctgcaaaccc tggtattgcc
     2401 aggttgcccc attgtcaact gatctcacta cccctcccgc accaagagac caaccctgca
     2461 atccaatgta aatatcccca ttaggttcaa ttgcaatatc tcttacagat tcacttacat
     2521 gattatatgt ttgaaaaagc gtatcgaacg ttattcctcc atcatacgtc ctgagcagca
     2581 atgcaccaag cgtttgggag catccaatta ataacgaatc attgccaaaa ctatagaact
     2641 tcgtgatatg acacattgaa ttaaagggta ttgactgcca ggtctgccca ttgtcggttg
     2701 ttttccaaaa agtagtggca ccttgagagc taacatacac atgccctgtt ttatcaattc
     2761 caattgcaag aattgaaata gtgcctgtat ttaacacatg ctgccaggtt tgtcctctgt
     2821 cagttgagcg ataaacccca tccgaaacac atggccctgt agtacctgtt cctacaaaaa
     2881 tatgcccttg gtcattcacg gccacgcacg agatatccaa tgtatcagga aacggcaatg
     2941 tctcccagaa atcctgcgct tcaccattta aggcaaccat caaactggct aatacaaaga
     3001 gtaatatttt atgtttcatg gggtggttgt ttgtttgaga tgcggcatat attataagca
     3061 ttcaaatata atagattttc ttcgttccct gcaacttggt tttgaagtgc agaatgtgta
     3121 tttcttttaa agttttacg
//
LOCUS       DAOPYB010000286         3131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_42421_length_3131_cov_8.356957, whole genome shotgun sequence.
ACCESSION   DAOPYB010000286 DAOPYB010000000
VERSION     DAOPYB010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42421_length_3131_cov_8.356957"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /gene="lipA"
                     /locus_tag="PKH58_13135"
     CDS             complement(<1..757)
                     /gene="lipA"
                     /locus_tag="PKH58_13135"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNX90014.1"
                     /translation="MTYLRKPAWLKTNLQSTENFTLVNKIVKENNLHTICTSGRCPNM
                     CECWNRGTATFMILGEICTRACRFCNTLTGKPLPIDSEEPRKVAESIRLMKLKHAVIT
                     SVDRDDLHDKGTAHWAETIRVIKTVNPQLTLEVLIPDFDGDTALIDRVIAEKPEIISH
                     NLETVKRLTTQIRTKAKYETSLSVLKHIASSGATAKTGIMLGLGETEEEVLELMDDAL
                     ATGCSVLTIGQYMQPSRKNIAVSEYIHPDKFAEY"
     gene            complement(1196..1780)
                     /locus_tag="PKH58_13140"
     CDS             complement(1196..1780)
                     /locus_tag="PKH58_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX90015.1"
                     /translation="MTERISKTKKQLIDAARELFASYGKKNLTMNDIAEASHKGRRTL
                     YTYFKNKDEVYKAVVDNEIDIIIEKLTNVSKKNLSPYLRLKELIITHLYTVKNSVTRN
                     GTLRADFFRDIYAVERSRRRIDIIELGLIKNILKEGVEKNEFKKMDVDTISIIILYAL
                     KGLEVPYIRQNLGTEPEKSNTDIIEFIFRGIKEI"
     gene            complement(1859..2809)
                     /locus_tag="PKH58_13145"
     CDS             complement(1859..2809)
                     /locus_tag="PKH58_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNX90016.1"
                     /translation="MPTTTERLKQSVLSSLPKAKKTVIWLLKIIIPISLGVSILQYLG
                     FIDLIAGYLTPVFSLIGLPGEAAIVFITSIFMSLYAPIAILTTLPLSPREITILAVMC
                     LITHNMIVETAVQSKTGSNYAVMFILRVGSSFVAAYFLNLLLPAQMGTSSVTVDAANY
                     DTFLQMLQGWLISSFWLIVKVVLIITGLMMLQNILKEFRWLDVISRSLAPLMSAMGLP
                     RSTSFLWFVAHILGLTYGSAVMIDAVENGELSKPDANFLNFHIAVNHSTLEDTLLFAA
                     IGVPVLWMIIPRFVLAILIVWIVRLWHFVWQKQKPVTENY"
BASE COUNT          884 a          612 c          668 g          967 t
ORIGIN      
        1 tatattctgc aaatttgtcg ggatgaatgt attcgctcac cgctatgttt ttgcgggagg
       61 gttgcatata ttgcccgatg gtaagaacag aacatcctgt tgccagggca tcgtccatca
      121 gttcgagcac ttcttcctcc gtttcaccca gtcccagcat gatgcctgtt ttggctgttg
      181 ctccgctgct tgcaatatgt ttcagtacac tcaggctggt ttcgtattta gctttcgtac
      241 gtatctgtgt agtcagtcgc ttcacggttt ccaggttgtg cgaaataatt tcgggctttt
      301 cggcaatcac ccggtcaatc agtgctgtgt cgccatcgaa gtcgggtatc agtacttcca
      361 gcgtaagttg cggattgacg gttttaatta ccctgatagt ttcagcccag tgtgcagttc
      421 ccttatcgtg caggtcgtcg cggtctacgg aagtaattac tgcatgtttc agcttcatca
      481 gccggattga ttcggctact ttacggggct cttcggaatc aatggggagt ggcttaccgg
      541 tcagcgtatt gcaaaaacgg catgcacggg tgcatatttc acccagtatc ataaatgtgg
      601 ctgttccccg attccagcat tcgcacatgt tggggcatcg tccgctggtg cagatggtgt
      661 gcaggttatt ttcttttaca attttattta ccagggtgaa attttcagtg ctttgtaggt
      721 tggttttcag ccatgccggt tttcggaggt atgtcatttt ttatctgtat gctaaatatt
      781 ttaaggggtg gactgctaaa taaatccggt ttccgggtct tgctcagtca cgttattcaa
      841 cggtgcaaat ttagaaaaaa acaaaacgtc cggcaaatgt ttaacagaac ggtttcagtt
      901 aagacgtatg ttaatgatga ctgtttctct ggaaaatgta tagcgttttt tgtaatcttt
      961 gtcttaattt aaactacgtc accgagcgaa ttaacaatac ggataaataa atcaaatttc
     1021 atttttttca gactaaagaa aaactgaaat tcaaacagga caagaaaagt gtacggttaa
     1081 gtttaacgaa agtgcctgat aaaacagatt acgtggtgga actgaccaac aaataagcca
     1141 acggaaactg aaaaagctgc ctatcgggca gccttttttt aagtttaatc ctgaattaaa
     1201 tttcttttat tcccctgaat ataaactcga taatgtcggt attgctcttt tcaggctcag
     1261 tccccagatt ttgacggata tagggaactt caagtccttt taaagcatat agaataatga
     1321 tcgaaatggt atcaacatcc atttttttga attcgttttt ctcaacaccc tctttcagaa
     1381 tgttttttat cagtcccagt tctattatgt caatgcggcg gcgcgaacgc tctacggcgt
     1441 aaatatctct gaagaaatca gcacgtaggg taccgttgcg ggtaactgaa ttttttacag
     1501 tgtaaagatg agtgatgata agttccttca gcctgaggta agggctgagg ttctttttgg
     1561 atacgttggt aagcttttct attataatat ctatctcgtt gtcaaccacg gctttgtaca
     1621 cttcgtcctt gttcttgaaa taggtgtaaa gtgtacgtct tcctttatga gaagcttcag
     1681 cgatatcgtt catcgtcagg tttttcttcc catacgaagc aaataactcc cgggctgcat
     1741 caatcagttg tttttttgtt tttgaaatcc gttcagtcat tttatgttta tttaatgggg
     1801 tctaaattag tattttaatc tcatttgccg taaatctgca gttggttttt aaaaaatatt
     1861 aataattttc tgtcaccggt ttttgttttt gccagacaaa gtgccacagc cgtactatcc
     1921 atactatcag aatggcgagt acaaacctcg ggataatcat ccaaagtaca ggaacaccga
     1981 tggctgcaaa caacagggta tcttcgaggg tggagtgatt gacagcgatg tgaaagttca
     2041 gaaaattagc atctggttta cttagttcac cgttttccac agcatcgatc attacagctg
     2101 aaccgtaagt tagtcccagt atgtgggcaa caaaccaaag aaatgaagtg gagcgtggca
     2161 gtcccatcgc gctcatcagc ggagccagtg aacgggaaat cacgtccagc cacctgaact
     2221 ctttcagtat attctgaagc atcatcaggc cggtgatgat aagcacaacc ttcactataa
     2281 gccagaacga actgatcagc catccctgaa gcatttgaag gaaggtgtcg taattcgcag
     2341 cgtcaacagt aacggatgaa gtgcccatct gtgccggaag cagcaggttc agaaaatatg
     2401 cagccacaaa ggacgaaccg acacgcaata taaacatcac agcgtaattc gatcctgttt
     2461 tgctctgaac agcagtttcc acaatcatgt tgtgagttat cagacacatt actgccagta
     2521 tggttatttc gcggggactg agcggcaggg tggtaagtat ggcaatggga gcatacagtg
     2581 acatgaagat acttgtgatg aaaacgattg cagcttcgcc cggtaatccg attaatgaaa
     2641 agacaggtgt cagataaccg gcaatcagat cgatgaatcc cagatattga agtatgctca
     2701 ctcccagact gatgggaatg attattttga gaagccagat gactgttttt tttgcttttg
     2761 gcaatgatga cagcaccgac tgttttaatc tttcagtggt tgttggcatg tgagcgtatg
     2821 atttgcttaa aaaaagaaag gttgtctccc gacaacccaa tcttcaatta accttaaatt
     2881 taataccatg aaaaacacga tgcaaagata caatgttttt tatgttataa aacatgttta
     2941 gaagaaataa ttatcgatat taacaacaat taacttaaaa acgataaata cagatatatc
     3001 ttactttaca gaacaagctt tttaatatat cagatcaaac gaacgaacaa atgtatgttt
     3061 tttttcggaa agagttgctt aaataatttg ccaatttgag aatgtgtcaa tttgaaaact
     3121 tgaaaaatag t
//
LOCUS       DAOPYB010000287         3075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_43566_length_3075_cov_6.404305, whole genome shotgun sequence.
ACCESSION   DAOPYB010000287 DAOPYB010000000
VERSION     DAOPYB010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43566_length_3075_cov_6.404305"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..776
                     /gene="frr"
                     /locus_tag="PKH58_13150"
     CDS             213..776
                     /gene="frr"
                     /locus_tag="PKH58_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNX90017.1"
                     /translation="MTEESKFILEEIKEGMQHALTHLEKEFHKFRTSKATPQMLEGVK
                     VEYYGNPTSVDKIANINTPDARTIVVQPWEKNMLNPVAKAILDANLGFNPQNNGEILR
                     IAVPPLTEERRRDLVKKAKAEAETAKVSIRSMRRTAVEDAKKLEKEGVSEDEIKTLEK
                     EIQHVTDGYIVQVDKILEHKEKDIMTV"
     gene            1402..2676
                     /gene="ltrA"
                     /locus_tag="PKH58_13155"
     CDS             1402..2676
                     /gene="ltrA"
                     /locus_tag="PKH58_13155"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HNX90018.1"
                     /translation="MLETIMERRNLEKALLQVERNKGVGGVDGMQTDALRDYLSANYL
                     QLRHQVLSGTYIPSPVKKVEIPKPQGGVRMLGIPTVIDRLLQQAISQWLAQFYEPNFS
                     KFSYGFRPKRNAHQAVMQAQTFLNEGKTRVIELDLEKFFDKVNHDKLMSLLMRKVSDK
                     PTLRLIRQYLRSGIMEGGVVSQRTEGTPQGSPLSPLLSNIILNELDTELTQRQHSFVR
                     YADDCSIYVRSKKSAHRVLKSITKYIEESLKLKVNKEKSKVSRPVESTLLGFSFYNDK
                     GRWTIRIAKKSIKRIKEKSKSITARNNGMNTAQKIAKMRPIIKGWVNYFSIAKAKFVM
                     QDLDECVRLRLRMGLWKEWKNCKTRVRHLQELKASKQKSYEWGNSSKGYCRVAHSPIL
                     CRTLNNAYFIKLGYVSFYRTYVDLTETQPSLF"
BASE COUNT         1037 a          589 c          726 g          723 t
ORIGIN      
        1 aggggttata gggattgatg ggattgaagg gattgaggcg aagcgggttt acagggtttg
       61 aaaggattga aaggattgat gggattgatg ggattgaatt attacttaaa cctaagccca
      121 accgccaaaa tcatatcgtg gaaacacttt ttacagcttg ttattaaata tttcaaagca
      181 atttttaaat ttgcattaca aaacgaatag ccatgactga agaatcaaag tttattttgg
      241 aagagattaa agaaggtatg caacatgcgc ttacccatct cgaaaaagaa tttcataaat
      301 tccgtaccag caaagccact ccgcaaatgc tcgaaggcgt taaggttgag tattatggaa
      361 atccaacttc ggttgacaag attgccaata tcaatacacc cgatgcccgc actattgtgg
      421 ttcagccatg ggagaaaaat atgctaaacc cggtggcaaa ggctattctg gatgccaacc
      481 tgggttttaa tccacaaaac aacggagaga ttttacgtat tgcggtacca ccgcttaccg
      541 aagagcgccg ccgcgacctg gtgaaaaaag ccaaggccga agccgaaacc gccaaggttt
      601 cgattcgcag tatgcgccgc actgccgttg aggatgccaa aaaacttgaa aaagaaggtg
      661 tttcggaaga tgaaataaaa acactggaaa aagaaataca gcatgttacc gatggctata
      721 ttgttcaggt tgataaaata ctggagcata aagaaaagga tatcatgacc gtgtaatcag
      781 ttacaaccat aaatataaag agcagctttc gggctgctct ttgtatttta gtgtgtcggg
      841 catggtcata gtctctaggg tgcaagtccc gaatgagtgt tgcagtacat atcattagcc
      901 aagagcaagg gtgtcgcggg tgactgcgaa tctgaaagaa gcaggcggca aatattcgag
      961 cctacgaaca gaaacttcat ataaggcggg gtggtgtggg tgatgttgct aaagaaacaa
     1021 aagcccaata ctgcacagaa caccataacg taaatgaaga ggctacatga atagaaaggc
     1081 tatgatctta ccccgagaga tctgacactg agtaggcgga ggtatgcgcg aatgcccgag
     1141 cgacaacgga tgcagtgatg caaaccggca ctgtcagaag tcagcagagg ccatagtagt
     1201 gggcgatttt ccacgaaggg ctgaatgtta aagtaaggga acgaactaac aaagtattaa
     1261 gggaaaggcg ataaaatccg aaacacgtaa gagaactgct tgcctgagga taggtcggaa
     1321 accgaaagta aaatgcaaga ggagactagc caccccgtga aacagtaagc aagcgcccct
     1381 gtggattaaa agttaaagta gatgcttgaa accattatgg aacggcggaa cttggagaaa
     1441 gccctgttac aggtagaacg taacaagggg gtaggaggcg tagacggtat gcagaccgat
     1501 gcacttcgcg actacctaag cgccaactac cttcaactcc gccatcaagt gctatcaggc
     1561 acctacatac ccagccctgt gaagaaagta gagataccca aaccacaagg aggagtaaga
     1621 atgttgggca ttccaacagt aatagacagg ctattacaac aagccatcag ccaatggttg
     1681 gcacaatttt acgaaccaaa tttttcgaag ttcagttatg gctttcgtcc gaaacggaac
     1741 gcccatcaag cagtaatgca agcacaaacc tttttaaacg aaggtaaaac gcgagtaata
     1801 gagcttgatt tagagaaatt cttcgacaaa gtgaaccatg acaaactgat gagtttgctg
     1861 atgcgtaaag taagcgataa accgacctta cggcttattc gccaatacct acgcagtggc
     1921 atcatggaag gcggagtagt gagccaacga acagaaggca caccacaagg cagcccatta
     1981 agtccgttac tgtccaatat catacttaat gagttggata ctgaattaac ccaacggcag
     2041 catagttttg taaggtatgc cgatgattgc agcatttatg tacggagtaa gaaatcagca
     2101 cacagagtgc ttaaaagtat cacaaagtac atagaagaaa gcttaaagtt aaaagtaaac
     2161 aaggaaaaga gcaaagtaag ccgtcctgta gagagtacgc tattagggtt ttcattttac
     2221 aatgacaaag gaagatggac catccgtata gcgaagaaat ccattaaacg catcaaggag
     2281 aaaagcaaat cgattaccgc gaggaataac ggaatgaaca ctgcgcaaaa gatagcgaag
     2341 atgagaccca tcataaaagg ctgggttaac tatttttcga tagccaaagc aaagttcgtt
     2401 atgcaagatc tcgatgaatg tgtcaggtta cgactaagga tggggctctg gaaagaatgg
     2461 aagaactgca aaacgagggt gaggcattta caggaactta aagcaagcaa acagaagtca
     2521 tacgaatggg gaaatagcag taagggatac tgtagagtag cccacagccc cattctatgc
     2581 cgcacactaa acaacgctta ctttatcaag ctgggatacg ttagttttta tcgtacgtac
     2641 gtagatttaa ccgaaacaca gccatcatta ttttaacaaa ccgccgtatg ccgaacggca
     2701 cgtacggtgg tgtgagaggt cggaaaataa aataggaggt aaacctattt tattttcctc
     2761 ctactcgatt ttatctttcc acttcaaata atctggataa attagtcaga gggaacctgc
     2821 atgaaatttt catccggttt ttgttaaata gattttttca tgtaggtttg gcaaaagttt
     2881 ttgtagagag aatgaaattc tagatgcgat ggatattgaa aagcgcgact cactgagtat
     2941 tgttgtgaat tcaacggcat tttacctgct ggcatacctc actgtatttt tgcttttcca
     3001 gtttgctacc atcattgcat caaatgcatt cgatatacct aacactttgt attataaccg
     3061 gataagtttc aatgt
//
LOCUS       DAOPYB010000288         3059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_43883_length_3059_cov_13.097870, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000288 DAOPYB010000000
VERSION     DAOPYB010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43883_length_3059_cov_13.097870"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKH58_13160"
     CDS             <1..363
                     /locus_tag="PKH58_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90019.1"
                     /translation="TGLRFYPGVFYEDVLFSMLLYLQAKRVSSIRRTFFHRRIRSNSV
                     MTSRLSHRNINDRILICNELLSNKKNFSDATSRHLLNLEVRNMMKFLVKNAIIGKHYK
                     LLFSYLNQILALFLKTFS"
     gene            complement(364..1329)
                     /locus_tag="PKH58_13165"
     CDS             complement(364..1329)
                     /locus_tag="PKH58_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HNX90020.1"
                     /translation="MKKTIRIYFVDFWNGFDPGDNFFINRLKIHYNVVLDEAHPELLF
                     YSYNGIQHFRYDKSITIYFSGENDVPDFNLSDYAISFHPVQFEDRHLRLPLFVLYKCF
                     PDLDKQKTSSSKELLQRKFCAFVVSNSKGADRKREDFFHKLNEYKKVDSGGRYLNNTG
                     SPVADKLEFIKGYKFVIAFENSAASGYTTEKLIEPLAVNSVPIYWGNPNVNKDFNSKS
                     FVNAADFKSLDELVQEIIRLDNDDDAYLSMAQEHPFTESQKKSADWINDFDEFMDSII
                     QQEPENAIRRTRFGYARFYTRRYKLLFGMANLPGIRYLVRKLSYR"
     gene            complement(1326..2777)
                     /locus_tag="PKH58_13170"
     CDS             complement(1326..2777)
                     /locus_tag="PKH58_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNX90021.1"
                     /translation="MGDNLKEKTISALKWSTVDRFGQQGVQLLLTLILAQLLSPDDFG
                     TIGVITVFTALSFIMVESGFGQALIRKKDADDKDFSTIFYFNIGLAVVLYLILFFLSS
                     LIANYFHQPVLENLSHLLFISILFNSMYLVPFVKLGAKLDYKSIAIVNIISTTFSGIT
                     GVTLAFLGYGVWALAWQTVLYHLVRVICFHYFVKWKPVLFFSFHTIKELSSFSLNLLG
                     TGILNIVFSNIYILILGRTFPIKETGYYTQANKFSETSNYTFSSILSGSTYNLFVQIY
                     DQTERLKRVFREAIRKTALIVIPVTLFLIAVAHPLIVTLFRDKWISIVPYYQIICAAN
                     LFAPLYMINYNLLNAKGLTKTTFRIEIFRKSAILVSIFICFRWGIIAMLTGYMLVNWL
                     VFFISMLQVKKTIDHYWRNQITDILPGLKIGLVMAICSYSLSFLISLPILLLATQGFL
                     AATLYVFYLKKTENELYNKGIHFLKSKWQILNQ"
     gene            complement(2848..>3059)
                     /locus_tag="PKH58_13175"
     CDS             complement(2848..>3059)
                     /locus_tag="PKH58_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNX90022.1"
                     /translation="TGGNSGSPVFNAKGELIGLAFDGNWEALSGDIVFEPNLQRTISV
                     DIRYVLFVMEKVGGADRLIKELTIK"
BASE COUNT          971 a          604 c          568 g          916 t
ORIGIN      
        1 acaggattac gtttttatcc gggtgtgttt tatgaggatg tccttttttc gatgttgctc
       61 tatttgcagg caaaacgggt aagttctata cgacgcacat tctttcaccg ccgtattcga
      121 agtaattcgg tgatgacgtc ccgcctcagt caccgaaata ttaacgaccg gattttaatc
      181 tgcaatgaac tactcagtaa taaaaaaaac ttttcagatg cgacaagtcg ccatttattg
      241 aacctggagg tgaggaatat gatgaaattc ctggtgaaaa atgcaattat tggcaagcat
      301 tataagttac tattttccta tttaaatcaa atacttgcac ttttccttaa aacgttcagc
      361 tgactatctg taacttaatt ttctcaccaa gtatcttatt cctggtaaat tcgccatccc
      421 gaataaaagt ttatagcgcc tggtataaaa ccttgcataa ccgaaacgtg ttcgtcgaat
      481 ggcattctct ggctcctgct gaatgatact atccataaat tcatcaaaat catttatcca
      541 gtctgcactc tttttttggc tttcagtgaa tggatgttcc tgtgccatac taaggtaagc
      601 atcatcgtcg ttgtccaaac ggataatttc ctgaaccaac tcgtccaagg acttaaaatc
      661 agcagcatta acaaaagatt ttgaattaaa atccttgttt acgttcgggt tgccccagta
      721 aataggaaca gaattaaccg ctaatggttc tatcagcttt tcggttgtat aacccgatgc
      781 tgcactgttt tcaaatgcaa ttacgaattt atatcccttg ataaattcca gtttgtcagc
      841 aaccggactg cctgtattgt tcaaatatct gccaccagaa tctacttttt tatattcatt
      901 caacttatgg aaaaaatctt ctctttttct gtcggctcct ttggagttcg acaccacaaa
      961 tgcacaaaat tttcgttgta aaagctcttt tgaactactt gttttctgtt tgtctaaatc
     1021 agggaagcat ttgtacaaaa cgaataacgg caatctcaaa tgccggtctt caaactgaac
     1081 aggatgaaat gagatggcat aatcactgag attaaaatcc ggtacatcgt tttcgcctga
     1141 aaaataaata gtaatgcttt tatcgtatct gaaatgctga atgccattgt aggaataaaa
     1201 caataattcc ggatgagctt catccaacac aacattatag tgaattttta acctgttgat
     1261 aaagaaattg tcgccggggt caaaaccatt ccaaaaatca acaaaataga tcctgattgt
     1321 ctttttcatt ggttgagaat ttgccatttg gattttagaa aatgaattcc cttgttgtat
     1381 aattcgtttt cagttttttt caggtaaaat acatacaaag tggctgccaa aaatccctga
     1441 gttgctaaaa gtaaaatcgg tagggagata aggaaagaga gtgaataact acaaatagcc
     1501 ataaccaaac caatttttag tcccggtaaa atatctgtaa tctggtttcg ccagtaatga
     1561 tcaatagttt tcttcacctg gagcatcgaa ataaagaata ccaaccaatt gaccagcata
     1621 tatccggtaa gcatggctat tataccccag cgaaaacaaa tgaaaattga aacaaggatt
     1681 gccgatttcc tgaaaatttc aatccgaaaa gtggttttgg ttagtccttt ggcattcagc
     1741 agattgtaat ttatcatgta aagtggtgca aataagttgg ctgcgcagat aatttgataa
     1801 tagggtacga tggaaatcca cttgtcacga aataaggtta cgattaaagg atgtgccacg
     1861 gctatcagaa aaagcgtaac gggtataacg atcagtgccg tttttcggat cgcctctctg
     1921 aatactcttt tcaatcgttc tgtttgatcg taaatctgta caaataaatt gtaagtactc
     1981 ccgcttaaaa tagaactaaa ggtgtaattg gatgtttcgc tgaatttgtt ggcttgagta
     2041 tagtatccgg tttctttgat ggggaatgtt cgccccaaaa tcaggatata aatattgctg
     2101 aaaacgatgt tcaatattcc tgtaccaagc aggttcaaac taaatgagga caactcctta
     2161 atcgtgtgga acgaaaaaaa taaaaccggt ttccatttca caaaatagtg aaaacaaatt
     2221 acccgcacca gatgatagag tacagtttgc cacgccagcg cccaaacccc gtatccgaga
     2281 aatgccagtg tgacgccggt aattccgctg aatgtagtgg atattatatt gactatggca
     2341 atgcttttat agtccaactt agctccaagt ttaacaaagg gaactaaata catggagtta
     2401 aaaagaatag aaatgaagag taaatgggaa agattttcca gtacaggctg atgaaagtaa
     2461 ttggcaatca gcgacgaaag aaaaaataag atgagataaa gaacaaccgc taatccgata
     2521 ttgaaataaa aaatggtact gaaatctttg tcatcagcat cttttttacg tatcagcgcc
     2581 tgcccaaatc cgctctccac cataataaaa gataacgctg tgaaaacagt aatcactccg
     2641 attgtgccga aatcgtccgg tgatagcagt tgagccagta tcagtgtcag aagcaattgt
     2701 accccctgtt gcccaaaccg gtcaacagtg gaccatttca gtgcgctgat ggttttctct
     2761 ttcagattat ctcccatgtc gtatattaaa aaaaacagat tacaaagata gccattgtaa
     2821 tctgtttaaa aagaatgcct tgcgaaatta tttaattgtc agttccttta tcaatctgtc
     2881 ggcgccgcct actttttcca tcacgaagag tacataacgg atatctacac ttatggtgcg
     2941 ttgcaggttg ggttcgaata cgatatcgcc actcagcgct tcccagttgc catcaaatgc
     3001 cagcccgatc aattctcctt tggcattgaa taccggactg cccgaattac caccggtga
//
LOCUS       DAOPYB010000289         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_44040_length_3052_cov_8.377377, whole genome shotgun sequence.
ACCESSION   DAOPYB010000289 DAOPYB010000000
VERSION     DAOPYB010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44040_length_3052_cov_8.377377"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..2103
                     /locus_tag="PKH58_13180"
     CDS             262..2103
                     /locus_tag="PKH58_13180"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNX90023.1"
                     /translation="MGKTTLLKMVILVILFLTGNNVMQADTYSHTITAKTWSAYDTQT
                     LTGVSWTASATGGSYWGYDATKGQQFGSSGSPATALSLTTNGISGTVTSIKVSTSGGS
                     SVVGAVSISVGGTAFTPASTTISATNTEYTFTGSSSGQIVISWSQTSSKALYLKALNI
                     TYSASNNPTIAASPASLSFSSNLNVTSASQAVTVTGTNLTSAPTYSITGTDAAQFSAT
                     GSLTTAGGNLDVTFTPTSVGAKTATLEIKSGTLTKTVALSGTGIDLTNPYNLDDSSPL
                     TSLNETFGDGTAIPTTLPSGWKSVLVQGNKNWIMRLFNSNNYAQMTAYEGSGVCQTLL
                     ISPAINFDVIQKNNVKFDWLAGFANGATLKVYVMQKDGTKTEVKAINATKPASGYETA
                     FTTETLDLSAYSGVKFLVFEYNGNTTTPATTTYQIDNVVASKVQATITITETTIPAMS
                     AEVNATDTKTINVGGSGLSENINLNITGADAAMFGVSPATLTLEGNAVPDQFVTITYK
                     PTAAGSHNAILEITSIGVDTKTMALTGSATDVGTGNAKVQQTAKVWVANGKIMVEAAA
                     GEAIEIYNAIGQKLVSRIASESLNEIDLYSKGVHLVKVSNCVARVLL"
     gene            2382..>3052
                     /locus_tag="PKH58_13185"
     CDS             2382..>3052
                     /locus_tag="PKH58_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX90024.1"
                     /translation="MRKFTLLLAMFFVMSFAVKAATYTIDFENEKKSAYASADVNLGG
                     VDSIKWNLTDVIIAKASMGNADYYEGTGSLRMRGYTTSVATMLANKIGGIGNISFKYK
                     VYGTDSQIPYNIDWSSDGTTWINIGQITANSTVQTFSFDLNQANARIRIIAAGTSTSN
                     KRMNIDNIVMTDNGGLPVSQIPVIIPASGNIYVPTTVTITAGTGASIYYTTNGTEPTN
                     ASTPYT"
BASE COUNT         1011 a          569 c          568 g          904 t
ORIGIN      
        1 aatatttatt aattattaaa tcatgtttca tctgttttca ttaagaatta tttatcgatt
       61 atttattgag tatgaaatat catctaatgt ttgtctcatt atttacaaat ggttcacaaa
      121 attgtaatat atgaaacaat gtgaagtaaa tatgaataag atgaggaaat aatattttaa
      181 atatcagaaa gtatattata tctttgctac ttttcaaaaa atacgaaatt ttaatttaaa
      241 tatttattaa atgatttaat tatggggaaa actactttat tgaaaatggt gattctcgtt
      301 atcttatttc tcactggaaa taatgtaatg caggcagata cttacagtca tacaatcaca
      361 gcaaaaacat ggtctgcata tgatactcaa actttaactg gagtttcatg gactgcatca
      421 gcaacaggtg gatcttattg ggggtacgat gctacaaaag gacaacaatt cggatcctca
      481 ggatctcccg ctacagctct ttctctaact actaacggta taagtgggac tgtaacatct
      541 ataaaagtgt ctacttcagg aggatcaagt gttgtaggag ctgtatctat ttctgtgggc
      601 ggaacagcgt ttactccagc atccaccact atttctgcta ccaatacaga gtatactttt
      661 actggttcgt caagtggaca aatcgtaata agttggtctc aaacttcatc aaaggcgtta
      721 tatttaaagg ctctcaatat tacttattct gctagcaata atcccactat cgcagcttcg
      781 cctgcttctc tgtcgttttc atccaatctc aatgttactt ctgcatcgca ggctgtgact
      841 gtaaccggaa caaatcttac atctgctccc acatacagca ttaccggaac ggatgccgct
      901 cagttttcag caacaggttc gcttacaaca gccggcggta atctggatgt tacattcacc
      961 cctacatccg taggtgcaaa aactgccaca cttgagataa aaagtggaac acttaccaaa
     1021 acggttgcac taagtggtac aggaattgat ctcacaaacc catataatct ggatgacagc
     1081 tcgcctctga cttcactgaa cgaaacattt ggtgacggaa cagccattcc cacaactctg
     1141 ccttccggat ggaaatcagt tttggttcag ggaaacaaga attggataat gagactattt
     1201 aattccaaca actacgcaca gatgacggca tatgaaggtt caggtgtttg ccaaacatta
     1261 ctcatttcgc ctgcaatcaa ttttgatgtt attcaaaaaa ataatgtgaa atttgactgg
     1321 cttgcaggat ttgctaacgg agcaactctc aaagtgtacg tcatgcaaaa ggacggaaca
     1381 aaaacagaag tcaaggcaat caacgcaacg aaacctgcaa gtgggtatga aacagctttc
     1441 accacagaaa cacttgactt gtcggcttac agcggagtga aatttcttgt tttcgaatac
     1501 aacggtaata cgacgactcc tgcaaccacg acttatcaga tcgacaatgt cgttgcttct
     1561 aaagtgcaag ccaccattac cattaccgaa actactattc cggcgatgtc agcagaggta
     1621 aatgctaccg atactaaaac aattaacgta ggcggatcgg gtttatctga aaatattaac
     1681 ctgaacataa caggcgctga tgccgctatg tttggagtta gtcctgccac tcttacattg
     1741 gagggaaacg ctgttccaga tcagtttgtt accattacat ataagcctac agcagcgggt
     1801 agccataatg ctatacttga aataacaagc atcggtgtcg ataccaaaac gatggcgttg
     1861 actggttcag ctaccgatgt aggtacagga aatgcgaaag tgcagcaaac agccaaagtc
     1921 tgggttgcca atggaaaaat catggttgaa gctgctgccg gtgaagccat agaaatctac
     1981 aacgcgatag gtcagaaact tgtcagccgt attgcttccg aaagtctgaa cgaaatagat
     2041 ttatactcca aaggtgtgca tctggtgaaa gttagtaact gtgtagcaag agtgctgttg
     2101 taatctttct gaaaataatt aacaccaata gcctgtttcg gtaatcggaa caggctattg
     2161 gtgtttttta acttattgaa tcaaggaaaa acaaggtttc aattgttaga ttttctaaag
     2221 ccaataaatt atatttcatt atccttttta gtttctattg gttagctaat attaaattac
     2281 gatgaattat attggaataa ttattaaaac aaaaaaaata ttatatcttt acattactta
     2341 atttccgaaa gaatattatt cactttaaaa acaataaatt tatgcgaaaa tttactttat
     2401 tattagccat gttttttgtt atgagttttg ctgtgaaagc agcaacatat acaattgact
     2461 ttgagaatga aaaaaaatca gcgtacgcaa gtgcggatgt caacttgggt ggcgttgact
     2521 ctattaaatg gaatctaaca gatgtaataa tagcaaaagc atcaatgggt aatgctgatt
     2581 attatgaagg aacaggatca cttcgaatga gaggttacac tacatcagtt gcaacaatgt
     2641 tagcaaacaa aattggagga ataggaaata tttcatttaa atataaagtt tatggaacag
     2701 attctcaaat tccatataat attgattgga gttctgatgg aacgacgtgg ataaatattg
     2761 gacaaataac ggcaaattct actgtacaga ccttttcttt tgacttaaat caggctaatg
     2821 ccagaatccg aataatagct gctggtacta gtacttcaaa caagcggatg aatattgaca
     2881 acattgtgat gacagataat ggtggtttgc ctgtttctca aataccggtt ataatccctg
     2941 catctggtaa tatctacgta ccaactacag taacaatcac tgccggtact ggtgcatcta
     3001 tttattatac taccaacggc acagaaccaa ccaacgcgag tacaccttat ac
//
LOCUS       DAOPYB010000290         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_45532_length_2985_cov_8.995563, whole genome shotgun sequence.
ACCESSION   DAOPYB010000290 DAOPYB010000000
VERSION     DAOPYB010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45532_length_2985_cov_8.995563"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1268)
                     /gene="nqrF"
                     /locus_tag="PKH58_13190"
     CDS             complement(12..1268)
                     /gene="nqrF"
                     /locus_tag="PKH58_13190"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HNX90025.1"
                     /translation="MNISAVIASLSVFLVVILILVIVLIIAKRYLVTSGDVKITINGE
                     KEVIAQAGSTLLNTLSTQSIFLPSACGGGGSCAQCRCQVTEGGGEILPTETVHFSRKE
                     IKDNWRLGCQVKVKNDISIKMPESILGIKKWECEVVSNKNVATFIKEFVVKLPNGEHL
                     NFVPGSYIQIDIPKYSLKYSEFDVEERFRADWDKFKMWDLTCTNNEETMRAYSMANYP
                     AEGDIVMLNVRIATPPFDKVNGGWMKVSPGIASSYIFNLKPGDKVMVSGPFGEFHPVY
                     NSKAEMIYVGGGAGMAPLRSHILHLLKTLKIRDRKISYYYGARALNEVFYMEDFLDLQ
                     KEFPNFSFHLALDRPDPVADAAGVEYTAGFVHQVLEQTYLKNHEAPEDIEYYMCGPGP
                     MAKAVEDMLWDLGVPRENLRFDDFGS"
     gene            complement(1329..1946)
                     /gene="nqrE"
                     /locus_tag="PKH58_13195"
     CDS             complement(1329..1946)
                     /gene="nqrE"
                     /locus_tag="PKH58_13195"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNX90026.1"
                     /translation="MENIANIFVKSIFVDNMIFAYFLGMCSYLAVSKNVKTSLGLGVA
                     VIFVLTITLPVNYLLENFVLKEGALAWLSPGLASLDLSYLAFILFIAVIAAMTQLVEM
                     VVEKFTPSLYASLGIFLPLIAVNCAIMGGSLFMQQRAFNNIGEATSYALGSGIGWMLA
                     IVGLAAIREKMEYSDVPKPLRGLGITFITVALMAIAFLAFSGLKI"
     gene            complement(2005..2637)
                     /locus_tag="PKH58_13200"
     CDS             complement(2005..2637)
                     /locus_tag="PKH58_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNX90027.1"
                     /translation="MSKLFSAKTKEVFLGPLSKNNPIGVQVLGICSALAVTAKLEPAI
                     VMGLSVTIIVAFSNVIISLLRNTIPNRIRIIVQLVVVAALVTVVSEFLKAFAYDVSVQ
                     LSVYIGLIITNCILMGRLEAFAMSNKPWESFVDGLGNGFGYAVVLIIVAFFRELLGSG
                     TLYGIRIIPQAVYDAGYMNNGLMLMPPMALIILACVIWIHRAKNKDLQEK"
     gene            complement(2634..>2985)
                     /gene="nqrC"
                     /locus_tag="PKH58_13205"
     CDS             complement(2634..>2985)
                     /gene="nqrC"
                     /locus_tag="PKH58_13205"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HNX90028.1"
                     /translation="AGLWGPIWGYLALNDDKNTVYGAFFSHASETPGLGAEIAQKGFQ
                     SEFIGKKIMNSENKFVSIAVVKNGQTAEGMDYVDGISGGTITSKGVDAMLMNCIGQYE
                     TFLQQPSITGGVQK"
BASE COUNT          858 a          685 c          596 g          846 t
ORIGIN      
        1 atgtattcgg attaacttcc gaaatcgtca aatctaagat tctcacgtgg tactcccaaa
       61 tcccagagca tatcttcaac agcctttgcc atcggacccg gaccacacat gtaatattca
      121 atgtcttcgg gtgcttcgtg attcttcaga tatgtttgtt ccaatacctg atgaacgaat
      181 ccggctgtgt attccactcc ggctgcgtca gccacaggat caggacggtc gagcgccagg
      241 tgaaatgaga agtttggaaa ttctttctgt aaatccagaa aatcttccat atagaatact
      301 tcgttgaggg cgcgggctcc ataataatag gaaattttcc ggtctctgat ttttaatgtt
      361 ttcagtaaat gaagaatatg cgagcgcaat ggagccatac cggcacctcc tccaacgtat
      421 atcatttcag ctttgctgtt gtaaacggga tggaattcgc caaaaggacc tgaaaccata
      481 actttatcac cgggtttcag attgaaaata tacgacgaag ctataccggg gctaactttc
      541 atccagccac cgttcacttt atcaaatggt ggagtggcaa ttctcacgtt aagcatgaca
      601 atgtcccctt cagccggata gttggccata gagtaggcac gcatcgtttc ttcgttattg
      661 gtgcaggtaa gatcccacat tttgaactta tcccagtcgg cacggaaacg ttcttcaacg
      721 tcaaattcgc tgtatttaag actgtatttc ggtatgtcaa tctgaatgta acttcccggc
      781 acaaagttca ggtgttcgcc attcggtaac ttaactacga attctttgat gaaagtagct
      841 acatttttgt ttgagacaac ttcacattcc catttcttaa taccaagtat tgattccggc
      901 attttaatgg aaatgtcgtt ttttaccttt acctgacacc ccagacgcca gttatccttg
      961 atttccttgc gtgagaaatg aactgtttca gtaggaagaa tttcaccgcc tccttcggta
     1021 acctggcatc tgcattgcgc acacgatccg cctcctccgc atgctgaagg aagaaatatg
     1081 ctttgtgtgg ataaggtgtt gagcagggta ctgccggcct gggctatcac ttctttttct
     1141 ccgttaattg ttattttaac gtcgcctgaa gttaccagat atcttttggc aattatcagt
     1201 acaatcacca gaatgaggat aacaaccaga aaaacactta aacttgcgat aactgctgat
     1261 atattaatgg ataatatcat tttaattata atttattcat ttacaatatt tactgttgaa
     1321 agaatgtctc agatttttaa tcccgagaat gccagaaacg cgattgccat cagggccacg
     1381 gtgatgaacg ttatacccag tccgcgtaat ggcttcggga cgtcggaata ctccattttt
     1441 tcgcgtatgg ccgccagacc tacaattgcc agcatccagc ctatacccga acccaatgca
     1501 tacgaagtgg cttctccgat attattaaac gcgcgttgct gcatgaaaag cgaaccaccc
     1561 atgatggcgc agtttactgc tatgagcggc aggaaaattc ccagcgaagc atacagcgaa
     1621 ggagtgaatt tttcgacaac catttcaaca agttgtgtca ttgccgcgat tacagcgata
     1681 aaaagaataa acgccagata actaagatct aaacttgcca gaccgggact taaccaggca
     1741 agagcacctt ctttcagcac gaagttttcc agcaggtagt taaccggcag ggtaatggtc
     1801 aatacgaaaa tcacagctac acccagcccg agtgaagtct ttacattttt agaaacagcc
     1861 aggtaagagc acattcctaa aaagtatgca aaaatcatat tgtcaacaaa aattgacttt
     1921 acaaatatat tggctatatt ttccatatat tgaattttat tgattgatgt ttatagtatt
     1981 tccaccagac agtgaaggtc ttgattattt ttcctgcagg tctttgtttt ttgcccggtg
     2041 aatccatatt acgcaggcaa gaatgataag agccatcggg ggcatcaaca tgaggccatt
     2101 gttcatgtaa cccgcgtcgt acacagcctg gggaattatt ctgatgccat acaaagtgcc
     2161 cgatccaagc aattcacgga aaaacgcgac gatgattaaa accaccgcgt aaccaaatcc
     2221 attaccaagt ccatcgacga aggattccca gggcttgttg ctcatggcga atgcttcaag
     2281 acgtcccatc aggatacagt tggttataat cagaccaata taaaccgaaa gctgaacact
     2341 gacatcgtaa gcgaatgctt taagaaattc gctgacaacc gttaccagcg cggcaacaac
     2401 aaccagctga acgataatac ggatgcggtt ggggatggta ttgcgcaaca aagaaataat
     2461 aacattcgaa aaggcaacaa taattgttac ggataatccc atcacaatgg caggttccag
     2521 tttggctgta acggcgagcg cagaacagat accaagtacc tgaactccga tcggattgtt
     2581 tttgctcaaa ggtcccagga agacctcttt tgtttttgct gaaaataatt tactcatttt
     2641 tgtactcctc ctgttattga tggttgttgt aaaaatgttt cgtattgtcc gatacaattc
     2701 atcagcatgg cgtcaactcc cttgctggta atggttcctc ccgagatacc gtccacataa
     2761 tccatgccct cagctgtttg tccgtttttt actactgcta tcgaaacaaa tttattctcg
     2821 ctgttcatta tttttttacc aataaattcc gactgaaatc ctttttgcgc aatctcagct
     2881 cctaaaccag gtgtttcgct tgcatgtgaa aaaaaagcgc cgtaaacggt gttcttatca
     2941 tcattcagcg ccaaataacc ccatatcggt ccccaaaggc cggca
//
LOCUS       DAOPYB010000291         2983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_45577_length_2983_cov_10.789617, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000291 DAOPYB010000000
VERSION     DAOPYB010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45577_length_2983_cov_10.789617"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PKH58_13210"
     CDS             complement(<1..263)
                     /locus_tag="PKH58_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX90029.1"
                     /translation="MNLKHIKYYSLVALTAVTFGATSCIGDLDVTPINPQVTQTFDKD
                     AVFAKVYAAFSLTGQQGAAGNNDIDIPDEGRYSLYRCLWNQNEL"
     gene            complement(287..>2983)
                     /locus_tag="PKH58_13215"
     CDS             complement(287..>2983)
                     /locus_tag="PKH58_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX90030.1"
                     /translation="YATVKKSDATGSVTAIKPDKMNKGLTTNAQDMIVGKIAGVSVTS
                     NGGAPGAGSTIRIRGGSSLNASNDPLIVIDGLALDNEGIKGVANFLSTINPNDIETFT
                     VLKDASATAIYGSRASNGVIIITTKKGTKGKMRVSYDGNMSVSTLSKQIDVLTGDEYR
                     DYAKKLYKDQPDVIAKLGTENTDWQSQIYQTALSNDHNVSLSGMAAMIPYHVSVGYTG
                     QNGILKTSNFQRVTGALNLNPTFFNDALKVNLSLKGMYAKTRYADTGAVGQAVAMDPT
                     QPVTSGLEPFKTKFGGYWAWYQTDDKIGVIQNPQATYNPVAGLMLNSDVAKSKDFIGS
                     AAFEYKLPFLPELTAHLNLGGEISSGKQDLLQPVTMGNRNNYYGRQGWDQIDKRNTSL
                     TYFMQYAKEIGVSNFDIMGGYEWQHFYREQQNDYKGLFKTDTNSDGVVDSKDDYYDHI
                     EKTKANNKYFATESYLVSFFGRLNYSLLNRYLFTATLRDDASSRFAKDTRWGLFPSFA
                     FAWKINEESFLKDVRSLSDLKLRLGYGITGQQNLQQGDYPYIPVYTNSTEGAFYQFGN
                     DYVITYRPDAYNPKLKWEETTTKNIGLDFGFLKSRITGSVDVYQRDTKDLINVVQIPA
                     GTNFRNKVISNIGNMTNKGVEFTINGKAISTNDVTWEIGYNVTYNQNKITKLTNGSEK
                     GYYVATGGLFQGSAQAHTVGFPSSSYYVYEQVYNANKKPIEGLFVDRSGDGIINEADK
                     YFYHNPNADFLMGLSSKLIVKRFDLGVSLRASIGNYNYNSVEAQRLNVGSAYVWSPLG
                     YFSNMTRSAMETNFSGKSTIQYMSDYFVQNASFVRCDNITLGYSFDKVLNVISGGRIY
                     ATIQNPFVITKYKGLDPEVYGGIDSNLYPRPTISIIGVSLNF"
BASE COUNT          821 a          621 c          627 g          914 t
ORIGIN      
        1 agttcgttct ggttccataa gcaacggtac agtgagtaac gaccttcatc gggaatgtcg
       61 atgtcattgt ttcctgctgc cccttgttgt ccggtcagtg aaaaagcggc atacactttc
      121 gcaaaaacgg catctttgtc aaaggtttgg gttacctgcg gatttatcgg cgtaacatcc
      181 aaatccccta tacaggaagt agcgccaaat gtaactgctg taagggctac cagggaataa
      241 tatttaatat gtttcaaatt cataattcta aaatttaata agttaattaa aagttaagac
      301 ttacgccaat aattgaaatg gtaggtctcg gataaagatt gctgtcaata cctccgtaaa
      361 cttcaggatc taatcctttg tattttgtga tgacaaaagg attttggatg gttgcataaa
      421 tacgtccgcc ggaaattaca ttcaacactt tatcgaacga ataaccaagg gtaatattat
      481 cgcaacgaac gaatgaagcg ttctgtacaa aataatcaga catgtactgg atggtagatt
      541 tgcccgagaa attggtttcc atggccgatc ttgtcatgtt ggagaagtaa ccaagtggtg
      601 accatacgta tgcactgcct acattcagcc gctgtgcttc cacagagttg taattatagt
      661 taccgatact tgctcttaat gatacaccca aatcaaaacg tttaacaatc aactttgagc
      721 ttagacccat gaggaagtcg gcgtttggat tgtgatagaa gtatttgtcg gcttcattga
      781 tgatgccatc gccactgcgg tcaacaaaaa gtccttcgat aggttttttg ttagcattgt
      841 aaacctgctc gtacacgtag tacgatgatg aaggaaatcc aaccgtgtga gcctgagctg
      901 aaccctggaa caatccaccg gtagctacat aatagccttt ttcagatccg ttagtcagtt
      961 tggtgatctt attctggttg taagttacgt tataaccgat ttcccaggtc acatcattag
     1021 tcgaaattgc tttaccgttg atggtgaatt caacaccctt gttggtcatg ttaccaatgt
     1081 tactgattac cttattgcgg aagtttgtac cggcaggtat ctgaactaca ttgataaggt
     1141 ctttggtgtc acgttggtaa acatctaccg atccggttat tctcgatttc aggaaaccga
     1201 aatctaaacc gatattttta gtagttgttt cttcccactt aagtttagga ttgtatgcat
     1261 ccggacgata ggtgattaca taatcgttac cgaattgata gaatgcacct tcagtgctgt
     1321 ttgtataaac cggtatgtaa ggataatcac cctgttgcag gttttgctga ccggtgatac
     1381 cgtaacctaa acgaagtttc agatctgaca gactgcgtac atctttgagg aaactttctt
     1441 cgttgatttt ccatgcaaaa gcaaacgaag ggaataaacc ccaacgggta tctttggcaa
     1501 atctcgaaga agcatcatca cgtagagtag ctgtgaataa atatctgttc agcagggaat
     1561 agtttaatcg accgaagaaa gaaaccaggt aactttcggt agcaaaatac ttattgtttg
     1621 ctttggtttt ttcgatatga tcgtaatagt catctttact atccactaca ccgtcagaat
     1681 ttgtatctgt cttaaacaaa cctttataat cattttgttg ttcgcgatag aaatgttgcc
     1741 attcgtaacc acccataata tcaaaattgc tcacgccgat ttctttggca tattgcataa
     1801 aataagtcaa agaggtattt ctcttgtcaa tctgatccca tccctgacgt ccgtaatagt
     1861 tattgcggtt gcccatagta accggttgca gaagatcttg cttaccgctc gaaatttcgc
     1921 cgcccaggtt caaatgtgca gttaattcag gaaggaaagg cagtttgtat tcgaaagcag
     1981 cactaccgat aaaatccttc gatttggcaa catcagaatt caacattaat cctgctacag
     2041 gattgtaggt tgcctgtgga ttttgaataa cgccaatctt atcgtcggtc tgataccatg
     2101 cccagtaacc gccgaattta gttttgaaag gttcaagacc tgaagtaaca ggctgagtcg
     2161 ggtccatggc aacggcctga cctactgcac cggtatcggc ataacgggtt ttggcgtaca
     2221 ttccctttaa actcaggttt actttcagag catcattgaa gaacgtcgga ttcaaattca
     2281 aagctccggt tacacgttgg aaattggagg ttttgagaat accattctga ccggtgtaac
     2341 ccacagaaac gtgataaggt atcatagctg ccatacccga taaacttacg ttgtggtcat
     2401 tgctcagggc tgtctgataa atctgacttt gccagtcggt gttttcggtt ccgagtttgg
     2461 caataacatc cggctgatct ttatacagtt ttttggcata atcacggtac tcatcaccag
     2521 tcaaaacatc tatttgtttg cttaatgtgc tcactgacat gtttccatcg tatgatacgc
     2581 gcatttttcc ttttgttcct tttttggtgg taatgatgat tacaccgttt gaagcgcgcg
     2641 aaccgtaaat ggcagtagca gaggcatctt tcaatacggt gaatgtttcg atatcattcg
     2701 ggttaatggt actcaggaag ttggcaacac ctttaatacc ttcgttatca agcgccagtc
     2761 cgtcaattac gatcaacggg tcgttgctcg cattcagcga tgatccgcca cgaatacgga
     2821 ttgtggaacc tgcacccgga gcaccaccgt tggatgtaac ggatacccct gcaatttttc
     2881 ctacaatcat atcctgagca ttggtggtga gacctttgtt cattttgtcg ggtttaatgg
     2941 ctgttaccga accggttgca tccgattttt ttaccgtagc ata
//
LOCUS       DAOPYB010000292         2978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_45698_length_2978_cov_6.603147, whole genome shotgun sequence.
ACCESSION   DAOPYB010000292 DAOPYB010000000
VERSION     DAOPYB010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45698_length_2978_cov_6.603147"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKH58_13220"
     CDS             complement(<1..248)
                     /locus_tag="PKH58_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90031.1"
                     /translation="MTVKQFISISSCLICLIFTASSENPDNLYQIDINGNHQVKGVNT
                     FRLGSNQNPAGDVIQINSNCWMLNGVPQLPVMGEFHYSR"
     gene            complement(245..2263)
                     /locus_tag="PKH58_13225"
     CDS             complement(245..2263)
                     /locus_tag="PKH58_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HNX90032.1"
                     /translation="MAEEIYKFLRPGYNFSKLKREKLDRGLVAVRESKNVVNISWRYF
                     SSDSITASFDIYRNGKKINKVPIANTTYFKDHKAPRRTLTYEIRTVGKSKKAPVESFS
                     KTLSDSAFTGYINIPLDIPAEGTTTLGQRYFYSANDASIGDVDGDGEYEIILKWEPSN
                     AHDNAHDGYSGNVYFDCYKLNGRKLWRIDLGKNIRAGAHYTQFMVFDLDADNKAEVVM
                     KTADGTKDGKGNIIGDPTADYRNKNGRILTGPEFLTVFEGQTGKAVHTTDYIPPRGNL
                     MSWGDDEANRADRFLACIAYLDGEHPSVVMCRGYYTRSVLAAFDYKDGKLTNRWVFDS
                     DSPGNEAYAGQGNHNLRVGDVDEDGCDEIIYGSCAIDNTGKGLYSTGMGHGDAIHLTA
                     FDPAGKKLQVWDCHENKRDGSSFRDAETGKVIFQVNSNIDVGRCMAADIDPNSKGVEM
                     WSIASQGIRNIGGEVINSSPWSVSMNMASWWDGDLSRELLDGKRITKYNPEKGVSEEL
                     FNCQDCSKNNGTKSNPCLSADILGDWREELLLRTADNKNLRIYLTPYETQYRFHTFLE
                     DPVYRISIATQNVAYNQPTQPGFFFGSDLGKCFPEKELTVNDDHVTLDAGNIYDSYQW
                     SIGGKGHLRYITTKDIPAGKKTKIEVTVSYRGYQFTDYVFVTLNPQKK"
     gene            complement(2261..2914)
                     /locus_tag="PKH58_13230"
                     /pseudo
     CDS             complement(2261..2914)
                     /locus_tag="PKH58_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
BASE COUNT          772 a          700 c          612 g          894 t
ORIGIN      
        1 cggctgtaat ggaactcacc catcacaggc agttgcggaa caccattcag catccagcag
       61 ttggagttaa tctgaatgac atcacctgca ggattttgat ttgagcctaa ccggaatgtg
      121 ttcacaccct tcacctgatg attgccgtta atatcaattt gatacagatt atcaggattt
      181 tcacttgaag cagtaaaaat caaacaaatc aaacaagaac taattgatat gaattgtttt
      241 actgtcattt tttttgggga ttgagcgtta cgaaaacata atcggtaaac tgataaccac
      301 gatagctgac tgtgacttca atttttgttt ttttacccgc cggaatgtct ttcgtggtaa
      361 tatagcgcag gtgcccttta cctccaatcg accattggta gctatcataa atatttccgg
      421 catcaagcgt tacgtgatca tcattcactg tcaattcttt ttcggggaaa catttcccca
      481 ggtcactccc aaagaaaaaa ccgggctgtg tgggctggtt gtaagccaca ttttgtgtgg
      541 caatactaat gcggtacacc ggatcttcga gaaaagtgtg gaagcgatac tgggtttcgt
      601 aaggagtgag gtaaatgcgc agatttttat tgtcggccgt tcgcaggagg agttcttcgc
      661 gccagtcgcc cagtatatcg gctgacagac atgggttcga tttcgtaccg ttgtttttcg
      721 aacaatcctg acaattgaaa agctcttctg aaacaccttt ctccgggtta tattttgtta
      781 tccgttttcc atccagcaat tctctcgata aatcgccgtc ccaccacgaa gccatattca
      841 tagatacact ccagggagag gaattgataa cttctccacc tatatttcgt ataccctgcg
      901 atgcaataga ccacatctca acgcctttag agttaggatc aatgtcagca gccatacagc
      961 gtccgacatc gatattgcta ttcacctgaa aaataacttt cccggtttcg gcatcgcgga
     1021 aagatgagcc gtctctttta ttctcatgac aatcccaaac ctgtagtttt ttgccggccg
     1081 gatcgaaggc agtcaggtgt atggcatcgc catgtcccat tccggtggaa tacaatcctt
     1141 ttccggtatt atcaatggca cacgaaccgt aaataatttc gtcgcatcca tcttcatcca
     1201 catcacccac ccgcaggttg tggtttccct gtccggcata cgcttcattg cccggagaat
     1261 cagagtcgaa cacccagcgg ttggtcagtt tgccgtcctt atagtcgaag gctgccagca
     1321 ccgaccgcgt ataatacccc ctgcacatga ccacacttgg atgttctccg tccagataag
     1381 ctatgcaagc caaaaaacgg tcagcccggt tggcttcatc atccccccaa ctcatcagat
     1441 ttcctctcgg aggaatataa tcggttgtat gtactgcttt tccggtctgt ccttcaaata
     1501 cagtcagaaa ttcggggccg gttagtattc tgccattctt atttctgtag tcggctgtcg
     1561 gatcaccaat aatatttcct tttccgtctt tggttccatc ggcagtcttc atcaccactt
     1621 ccgctttgtt gtcagcatcc aggtcgaaaa ccataaactg tgtgtaatgc gctccggcac
     1681 ggatattttt gcccaggtca attctccaga gtttccggcc attcagttta tagcaatcaa
     1741 aataaacgtt tccggagtat ccatcgtgtg cattgtcatg agcatttgaa ggttcccatt
     1801 tcaaaattat ttcatactca ccatcgccat ccacatcacc tatactggca tcattggcac
     1861 tataaaaata acgctgtccc aaagtggtcg taccttcggc gggaatgtcc agtggaatat
     1921 tgatatatcc ggtaaaagca gaatccgaca aagttttaga aaaactttcg accggggctt
     1981 ttttcgattt tcccacagtt cttatttcat acgtcaatgt tctgcgggga gctttatggt
     2041 ccttgaagta agtggtatta gcaataggca ctttattgat tttttttccg tttcggtaaa
     2101 tgtcaaagga ggcagttatt gaatcggacg aaaaatatct ccaactgata ttgaccacat
     2161 ttttactttc acgtaccgcc accaaacctc tgtccaattt ttcccttttt aatttactga
     2221 agttgtatcc gggtcggagg aatttgtaga tttcttcagc catcacccgg tgtccgatat
     2281 cattcggatg cagaaaatcg gcataataat cggctttatc aataagagga gtatttaaat
     2341 caatgatttt taatccttta tcattcgcaa ttttgcgaat cagcggaata atttcattcg
     2401 ttaaaactcc gggttgatgc tgccatcgtg tatttgggac gatgggaatg ggcaggcaga
     2461 ggtaaacggt tggttttgaa ggcaggttaa tgaactcatc gcagatatac gacaggtctt
     2521 tttcaaaatt ccgtgcaaaa tcgcccgtat tccaaactct tggctgcgag tcgttggttc
     2581 ccagcgcaat ggtaacaatg tcgggctgaa actcttttgc tttattgaat gcatcgagac
     2641 ccgtgtaggg tttatgtgtg ttccggcagg cagtggcacc gcttacacca aaattaccaa
     2701 cttcatacgc atcacccaac atggatgcca ggacgcccac atatcctttt tgagagcggt
     2761 tgcttgcacc ggcaccttcc gtaatactat tgcccacaca ggcaattttt gtttttttga
     2821 aaggagcaac tttatttttg ggatagtttt cctgagcata cacgcctata gtaagaaaaa
     2881 cggaaagaac aatcagcgac caggttgttt tcatttgttt ataatttatt tatcggcccc
     2941 ctacccccct aaagggggaa tattgagcgt atatcatt
//
LOCUS       DAOPYB010000293         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_45773_length_2975_cov_10.698630, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000293 DAOPYB010000000
VERSION     DAOPYB010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45773_length_2975_cov_10.698630"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKH58_13235"
     CDS             <1..306
                     /locus_tag="PKH58_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90033.1"
                     /translation="KQVLWLTEVAKICNTLLITTPNRWLPFETHSMTFFFHWFPEKVR
                     NFFYRKIGKSQFTDDYMWLLGEKEFKRIINEAGFEIISFYKNRFLFFTIDFVAICKS"
     gene            complement(437..1843)
                     /locus_tag="PKH58_13240"
     CDS             complement(437..1843)
                     /locus_tag="PKH58_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90034.1"
                     /translation="MKKIKCFLKYTVTALFLFVFMTNINAERKIRYSPLLVTDFVCKI
                     TNIQQTANNKLEFDVYLWDNDPAQTFEFAGGQMGIWFNSLIYSGGTLTVTYSNTNSTL
                     NTSQQFSSVPNILTKLNDQTLLKLAGRNPPGAGNGTIISHVSPGTLITHFIFTCSVPF
                     TSGTRPNFSFATSTYPLSIAEYINNTNTALTIVPGINAIVDGDPVLNPAPTAYTVSGS
                     GSYCQNGAGLPVGLSSSDLGVNYQLYNGATAVGTPMAGTGSTISFGNQIAGTYTVTAT
                     NTTTLVTTSMIGSAIITADPLITPSITIEASTNPSSPGVSVTFTPTPVNGGLNPAYEW
                     FLNNVSVGTSETYSYVPTDGDQVYAKMTSNDTSPCLIGNPATSNTITMSVSPGTVFTN
                     EKQLIINIFSANKIIYINSPVKAKQINVYNLLGSLIANEENVTGLRKINLNNFPNECY
                     IVKVVTDNSVTTSKVLLK"
     gene            complement(1932..>2975)
                     /locus_tag="PKH58_13245"
     CDS             complement(1932..>2975)
                     /locus_tag="PKH58_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNX90035.1"
                     /translation="TSYTFTNISENHTITAKFTLAQGIALNKPATAQSSEINFAPEKA
                     NDEDGTNNSYWGANPYPQWWQVDLQGVYDITGVYIRNYYDGDGRYYRYEILASTDNVN
                     FTKIAEKTSVTPSTNIGELYNLTTRARYIKVMMNYNSINQSVHISDFRVFGTQDKVLN
                     LSSVMLEGLYNGSNTMRKAQDETGAHWPGEVADHITVELHDAANYQNIVYTATDVELR
                     TDGNASITVPVNFNGNYYITIKHRNSLETTSATAVSFAGSTVNQSFGLPVNVYGGNLQ
                     LMTDGKYTIFAGDVNQDGVVDFNDYIEVDNDSYNYSTGYLSSDIDGNGIVDFNDYIHI
                     DNNNFNYISSMHP"
BASE COUNT          876 a          512 c          576 g         1011 t
ORIGIN      
        1 aaacaagtat tgtggttaac ggaggttgcg aagatatgca atacgttatt gataacaaca
       61 ccaaataggt ggctgccgtt tgaaacacat tctatgacat tcttttttca ttggtttcct
      121 gaaaaagtga gaaacttttt ctacagaaaa ataggaaagt cacaatttac tgacgattat
      181 atgtggttat taggtgaaaa agagtttaaa agaattatca atgaagctgg ttttgaaata
      241 attagcttct acaaaaacag gtttttgttt tttacaattg attttgtggc aatttgtaag
      301 tcctaatgtc tgcctgttgg tgtcattatt taattttcag acacattgaa aaaataaaaa
      361 ttctcaaatc cgttaagata tttgccggat ttgagaattt tctatgaaga ttaattgatt
      421 caaattgatt tcaaatttat ttcaatagaa ctttactggt agttacacta ttatcggtta
      481 caacttttac aatgtaacat tcattcggga agttgttcaa atttatttta cgaagtcccg
      541 ttacattttc ttcattcgca atcaaagaac ccaacaggtt ataaacattg atttgcttcg
      601 ctttcacagg actgttgatg tagatgattt tattggcaga gaagatgtta attattaatt
      661 gcttttcatt agtaaataca gtacctggac ttaccgacat ggtgatggta ttggaggtcg
      721 caggatttcc gattaggcag ggtgaggtgt cattcgaagt catctttgca taaacctgat
      781 caccgtcagt tggtacgtat gaataggttt ccgaggttcc aaccgaaacg ttgtttaaga
      841 accattcata agcaggattc aatcctccat taaccggtgt tggggtaaat gtgaccgaaa
      901 cacccggtga tgaagggttg gtgctcgctt caattgttat gcttggtgta ataagaggat
      961 cggcagtaat tattgcgctt ccgatcattg atgtggtcac taatgttgtt gtatttgtag
     1021 ctgttactgt ataagtaccg gcgatttgat ttccaaaact aatagtagaa ccagtcccgg
     1081 ccatcggtgt tccaacagca gttgcgccat tgtagagttg ataatttaca cctaaatcag
     1141 aactgcttaa tccaaccgga agaccagctc cattctggca gtaagaaccc gagcccgaaa
     1201 cagtataagc tgttggagca ggattaagaa ccggatcgcc atcaacgatt gcattgatgc
     1261 ctggaactat tgttagtgca gtattcgtgt tatttatata ctctgcaatt gaaagtggat
     1321 atgttgatgt agcaaaagaa aaatttggtc ttgttcctga tgtaaatggt actgaacatg
     1381 taaaaataaa atgcgttatt aatgttcctg gtgaaacatg agaaataata gttccatttc
     1441 ctgctcccgg aggatttcta ccggcaagct tcaacaaagt ttgatcattt aatttagtta
     1501 aaatattagg aaccgaactg aattgctgtg aagtattaag tgtagaattt gtattgctat
     1561 atgtcacggt taatgtacct ccagaatata ttagtgagtt aaaccatatt cccatttggc
     1621 caccagcaaa ttcaaatgtt tgagctggat cattatccca taaatacaca tcaaattcta
     1681 atttattatt cgctgtttgt tggatattgg ttattttgca aacaaaatca gtaactaata
     1741 aaggagaata tctaattttc ctttcggcat taatatttgt cataaataca aataaaaaca
     1801 atgctgtcac tgtgtacttt aaaaagcatt taattttttt cataatgtct ggtttttaca
     1861 aattgtatat tctcaatatc ttttgaacga aaaggtaaaa cagttatccg gtttattctc
     1921 ttattgattt tttaaggatg catactgctt atataattga aattgttgtt gtcaatatga
     1981 atgtaatcgt tgaaatccac aatcccattt ccatcaatat cggatgataa atatccagtt
     2041 gaataattat aagaatcatt atcaacttca atataatcgt taaaatcaac aactccatcc
     2101 tgattgacat ctccggcaaa gattgtatat tttccgtcag tcattagttg cagattgcct
     2161 ccgtaaacat ttacaggtaa tccgaacgac tgattaacag tactacctgc gaaggatact
     2221 gcggttgctg aagtcgtttc aagactgtta cggtgcttga tggtgatata ataattacca
     2281 ttgaagttta cgggaactgt aattgaagca ttaccatctg ttctcaactc tacgtcagtt
     2341 gcggtgtaaa cgatattttg ataattggca gcatcgtgca gttctacagt gatatgatcg
     2401 gcaacttctc caggccagtg agcacccgtt tcgtcctgag ccttgcgcat ggtgttactc
     2461 ccattgtaaa gtccttcgag catcactgaa ctcagattaa gtaccttgtc ttgtgtgcca
     2521 aataccctga aatctgaaat atgaaccgac tgattgatag agttgtagtt catcattact
     2581 ttgatgtacc ttgcccttgt tgtcaggttg taaagttcac cgatattcgt tgatggggtt
     2641 acggatgttt tttcggcaat ttttgtaaag ttcacgttgt cggtacttgc gagtatttca
     2701 taccggtagt atcttccatc tccatcgtaa tagtttctga tatatacccc tgtaatatca
     2761 tacactccct gaagatcgac ctgccaccat tgcggatatg gattggcacc ccagtagcta
     2821 ttgtttgttc cgtcctcgtc gtttgctttt tcaggtgcaa agttaatctc agatgattgt
     2881 gcggtagctg gtttgtttag cgcaatacct tgtgccaacg tgaatttagc cgtaatggta
     2941 tgattttcgg agatatttgt aaatgtgtag cttgt
//
LOCUS       DAOPYB010000294         2951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_46337_length_2951_cov_8.948895, whole genome shotgun sequence.
ACCESSION   DAOPYB010000294 DAOPYB010000000
VERSION     DAOPYB010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46337_length_2951_cov_8.948895"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PKH58_13250"
     CDS             <1..503
                     /locus_tag="PKH58_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90036.1"
                     /translation="GSIQGRVNMGQIFMNYIVNAGYLFGKAPFDLLDQPVGSMSLGYA
                     KDRFNLLHFASFAHNLYSNTHIYFNAGGVLLNKIPIIRKFKLREIISVKCHYGTLNNS
                     YKGVFDLPGYFSNSYKEPYTEVGFGITNIFKVLRFEYVTQLSSTYRNKSFTDNSGFRF
                     RAEMSF"
     gene            542..1336
                     /locus_tag="PKH58_13255"
     CDS             542..1336
                     /locus_tag="PKH58_13255"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX90037.1"
                     /translation="MKNLKGKVVWITGASFGIGEELAYAFAREGSFLVLSARNKEKLE
                     QVKVTCLESTPKCWVQPVDLSDTSQLESVAENVLALTGRIDLLINNAGRSQRSLAKET
                     SLEIDRNIMELNFFSVVALTKLVLPQMLKNGSGQIVVISSISGKLGFPMRTAYAASKH
                     AIQGYFESLRAELINDNIKITIVSPGRIKTNISVNALTKDGSAYNRMDEGQANGMRAG
                     LCAAKIVKAIKRERKEFLVGKTEILMVHIRRFLPALYYKLVTRIKN"
     gene            1366..2418
                     /locus_tag="PKH58_13260"
     CDS             1366..2418
                     /locus_tag="PKH58_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNX90038.1"
                     /translation="MRKNICGIQQIGIGVKNAKEAWKWYRQAFGMDIKVFEDTATAKL
                     MKHYTNGEECDRYAALAMNMEGGGGFEIWQHTERNPNPPFFEVKLGDCGIFICKIKSR
                     NIKKSYRGHKEMGLSILGGLMPDPIGNLHYYLKDPYNNIFEVVEEKETYMKQKSLTGG
                     VAGVVIGVSNIEEALKVYSEILQYDTIVYYKTDIFEDFEVLPGGNERYRRILLKHEPR
                     TGPFSKLLGPTQIELIEPIDRKPVKIFQDRIWGELGYIHLCFDIIGMDLLEKECQQKN
                     FLFTVNSADSFDMGVAAGHFSYISDPDGTPIEFVETHKVPIIEKLGWYMNLKSRDPKK
                     SLPDWMIKTLIFNRVK"
     gene            2575..>2951
                     /locus_tag="PKH58_13265"
     CDS             2575..>2951
                     /locus_tag="PKH58_13265"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90039.1"
                     /translation="MFLQAFVNKKAIFGSISVFDINTEILAALKFKFPFVQVMKSIEE
                     CARKDVVFLALYPHVIMRTLQQIKDVVTEQTILISLTAKITIEQISSVVKCQQIVRIL
                     PTAISFINQGYNPVTFKQTMSGF"
BASE COUNT          972 a          474 c          633 g          872 t
ORIGIN      
        1 atggttcaat acagggacgg gtgaacatgg gacagatttt tatgaattac atcgttaatg
       61 ccggttattt gttcggaaag gctcctttcg atctgttgga tcagccggtt ggctcaatgt
      121 cgctgggata tgcgaaagac cggtttaatc ttctgcattt tgcctcattt gctcataatt
      181 tgtattctaa tacgcacatt tattttaatg ctggaggagt tcttctgaat aagattccga
      241 taataagaaa attcaaactg cgcgaaatta tttcggtcaa atgccattac ggtacattga
      301 acaattccta caaaggtgta tttgatttgc ccggatattt ttcgaacagc tataaagaac
      361 cctataccga agttggattc ggtataacaa atattttcaa ggtattacgt ttcgagtatg
      421 tgactcaatt gagcagtacc taccgaaaca aaagttttac cgacaacagc gggttccgtt
      481 tccgtgctga aatgagtttc tgatatggta caggttatct ttaaacaaca actctgaggt
      541 tatgaaaaat ttaaagggaa aagtcgtctg gatcactggt gcatcatttg gtattggtga
      601 agaattagcc tatgcttttg ccagggaagg ttcattttta gtcctgtcag ctcgaaacaa
      661 agaaaagctg gaacaggtca aagtcacgtg tttggaaagt acaccgaaat gttgggttca
      721 acccgtagat ctctcagaca ccagtcaatt ggagagtgtg gctgaaaacg ttttggcatt
      781 aacaggccgg attgatttgt tgattaataa tgcaggccga agtcaacgct ctctggccaa
      841 agaaacatcg cttgagatcg accggaatat tatggaactt aattttttca gtgtggtggc
      901 tttaaccaag ctcgtcttac cgcaaatgtt aaaaaacggg tcaggacaga ttgttgttat
      961 aagtagcatc tccggtaaat taggctttcc catgcgaaca gcttacgcgg cctccaaaca
     1021 tgcaatacaa ggttattttg aatcgctcag ggcagaattg attaacgaca atatcaagat
     1081 aactatcgtt tctcctggaa ggatcaaaac gaatatctca gtaaatgccc tgactaaaga
     1141 tggttcggct tacaacagga tggacgaagg ccaggcaaat ggaatgcgtg caggattatg
     1201 tgctgccaaa atagtaaaag ctataaaaag agaaagaaaa gagttcttag tgggtaaaac
     1261 ggaaattctt atggtacaca taaggcggtt tttgccagct ctatattata aattggttac
     1321 gagaattaaa aattagaact tcagaattac aaaaatcaga ttgaaatgag aaaaaatatt
     1381 tgtggcattc aacagatcgg tattggagta aaaaatgcta aagaagcgtg gaaatggtac
     1441 cgacaagcat ttggaatgga tattaaagta ttcgaagata cggctactgc caagttaatg
     1501 aaacattaca ctaatgggga ggaatgtgat cgctatgcag ctttagccat gaatatggaa
     1561 ggaggtggtg gatttgaaat ttggcaacac actgaaagga atccgaatcc gccgttcttt
     1621 gaagtcaaac taggagattg tggaatattt atttgtaaaa taaaatcaag gaatataaag
     1681 aaatcttatc gtgggcataa agaaatggga ttatcaatat tgggaggttt aatgcctgat
     1741 cctattggta atttacacta ttacctcaaa gatccttata ataatatttt tgaagttgta
     1801 gaagaaaagg aaacctatat gaagcaaaaa tcattaaccg gtggcgttgc tggtgttgtg
     1861 attggtgttt caaatattga agaagcatta aaggtttact cagagattct tcagtatgat
     1921 acaattgttt attacaaaac ggatattttt gaggatttcg aagtattacc tggaggaaat
     1981 gaaagatacc gtcgcatttt attaaagcat gaaccacgga ctggtccgtt tagtaaattg
     2041 ttgggaccga ctcaaatcga attgattgaa ccaatagacc gaaagcctgt gaaaatattc
     2101 caggaccgga tatggggtga acttggttat attcacttgt gcttcgacat tattggaatg
     2161 gatttattgg aaaaagaatg tcagcagaag aattttctct ttacagtaaa cagtgctgat
     2221 agcttcgata tgggtgttgc tgccgggcac ttctcatata tttctgatcc ggacggaacg
     2281 cccattgaat ttgttgaaac acataaggta cccattattg agaaacttgg ttggtatatg
     2341 aatctgaaaa gcagagatcc taaaaaatca ctacctgact ggatgataaa aaccttaata
     2401 ttcaatcgtg taaaatgaca gaacaacgtt tcagtttcaa gaaagccggt tgagaaaaag
     2461 cgaataatta aagaaaggat tcttttcggt tccagtataa gatttattga acagaaaaag
     2521 aagtgtcatg aaaaaaaatc gttatgtttt attggaggag gtcgaattac aggcattttt
     2581 ttacaggctt ttgtaaacaa aaaagcaata ttcggctcta tttcagtttt tgatattaat
     2641 actgaaattc ttgcggctct aaaatttaaa tttccgttcg ttcaggtgat gaaatcaata
     2701 gaagaatgtg ccagaaaaga cgttgttttt ctggcgctct acccacatgt aattatgaga
     2761 actcttcagc aaataaaaga tgttgttact gagcaaacca ttcttatttc gttgactgcc
     2821 aaaatcacga ttgaacaaat atcgtcagtt gtcaagtgcc agcaaattgt gaggatttta
     2881 cccacagcca tttcgtttat caatcaggga tataacccag tgacatttaa acaaacaatg
     2941 tctggatttc a
//
LOCUS       DAOPYB010000295         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_46635_length_2939_cov_8.551664, whole genome shotgun sequence.
ACCESSION   DAOPYB010000295 DAOPYB010000000
VERSION     DAOPYB010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46635_length_2939_cov_8.551664"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="PKH58_13270"
     CDS             complement(<1..1439)
                     /locus_tag="PKH58_13270"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNX90040.1"
                     /translation="MKQIIFFLIFLLTVSVSLKAQQYSVSGKVTDSETGNALPFVNLV
                     INSSPHGSISDIEGNFVLKSSEPIHYIRASYVGYKPDTIETNASPVVNFSLQKSSIQL
                     NEVVILPGENPAHRIIDSAIAHRKENDPFQLDAFSYGSYSKFIVTAEMDSLMAIPDEM
                     LDSGHLELKQFFSKQHVFLMENMAERKYLRPGNDNEILTASRVSGFGDPMLTLLMSQI
                     QSFSFYKTLITISDKNYVNPISPGSTDKYFFLLEDTIYDNADTVFVISYRPRRNAHFD
                     ALKGLLYIHTAGWALQCVTAEPIEEEPGMSVRIKQNYTRPDSIHWFPSQLHTDILFSS
                     LKLYEYPVYGHGVTELKNIKINPALKKSDFAAAKVIIEPDAKLKDSLFWAENRPQPLT
                     QKEINTYHVIDSIGRKAHFDRLLNITTTLMSGFIPVGPLAFDINALYRNNVVEGGRPG
                     IGLKTNYRVSKNWSMYGYYGYGLRDQEHK"
     gene            complement(1481..2554)
                     /locus_tag="PKH58_13275"
     CDS             complement(1481..2554)
                     /locus_tag="PKH58_13275"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX90041.1"
                     /translation="MDTHLTNISVFAEIMGVDAKTLHKWYKNNLSGFEPDGAGQIHEH
                     DIEVMAAHGQTNIEVPILRPENIGANMGIDEKMIGEQYYTILSNRETGKIAFCAGTTR
                     SDYLKQAVEPLLDSLSQVKRITRDMAGCYARFSEEVMPNAIQVADKFHVINNLLDAMQ
                     AVRIRCRQKLLEQRRKAHQEFKESEKQRLIDCEKKHLKFTPKKFIYKEDRLANGETPG
                     ELLLRSHFLLYKFPNQWKLWQMKRAELMFRVFPEIKAAYYLTCSFRTWYSNENIGQNQ
                     LQTLKNLYQWYEDVDDADIDELSNFKSLVESNEDEIVEYFTNGETNAKAENLNGQIKK
                     FLASNKGIRNTDFFFFRLSKYFA"
     gene            complement(2502..2891)
                     /locus_tag="PKH58_13280"
     CDS             complement(2502..2891)
                     /locus_tag="PKH58_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90042.1"
                     /translation="MDKIISLIVPSEILESFDVSEVNESAIQIEIILEEKKDLIPEEI
                     KSFDAVLDGFCNPLSLLSFPIKGKPTYLVIKRRRWKLAGEDEHYSNSYQFNHPGIKAT
                     KEFAAFLKEVHGHTPDEYLRICRDNGC"
BASE COUNT          764 a          624 c          617 g          934 t
ORIGIN      
        1 aacttatgct cctgatcgcg gagaccataa ccgtaatatc catacatgct ccagtttttc
       61 gaaacacggt aattggtttt cagtccaatt ccggggcgac ctccttctac cacattgttc
      121 cgatacagcg cattaatatc gaaggccaaa ggcccaacgg gaataaatcc gctcatcaaa
      181 gtggttgtaa tattgagcag ccggtcgaaa tgggctttgc gtccaatact gtcgattaca
      241 tgataagtgt tgatttcctt ttgtgtaagc ggctgtggtc ggttttccgc ccagaacaga
      301 ctgtctttca gttttgcatc gggctcaatg atgaccttgg ctgcagcaaa atcggatttc
      361 ttcagcgccg gatttatttt gatatttttc agttcggtca cgccatgtcc gtacacaggg
      421 tattcgtaca atttcagcga gctgaacaga atgtcggtat ggagttgcga cgggaaccag
      481 tggatggagt cgggccttgt ataattctgt tttatccgga ctgacattcc cggctcttct
      541 tcgatgggct cggcggttac gcactgaagc gcccatccgg ccgtatgaat gtacagcagt
      601 cctttcagtg catcgaaatg cgcattgcgc ctgggccggt acgaaatgac gaaaacggta
      661 tctgcattat cgtaaatagt gtcttcaagc agaaagaaat acttgtcggt gctgccgggg
      721 ctgattggat tcacgtagtt tttgtcggaa atggtaataa gcgttttata gaaagaaaaa
      781 gactgaatct ggctcataag cagcgtcagc attgggtcgc cgaaacccga aacgcgtgat
      841 gccgtcagta tttcattgtc attgcccggc ctgagatatt ttcgttcggc catgttttcc
      901 atgagaaaaa catgctgttt tgagaaaaac tgtttcagtt cgagatggcc tgaatccagc
      961 atttcatcag ggattgccat cagggaatcc atttcggcag tcacgataaa tttgctgtaa
     1021 gacccgtaag aaaaagcatc gagctgaaaa ggatcgttct cttttctgtg agcgatggcg
     1081 ctgtcgatga tccggtgggc cggattttca cccggcagaa taacgacttc gttgagttgt
     1141 attgaagatt tctgaagcga aaaattcaca acaggacttg cgttggtttc gatggtgtcg
     1201 ggcttataac ccacataact tgccctgata tagtggatcg gttcggagga ttttagtaca
     1261 aaatttccct cgatatcgct gatggaacca tgtggtgagg agttgataac cagattgaca
     1321 aagggcaggg cattgccggt ttcagaatcg gtcactttgc ctgaaacaga gtattgctgc
     1381 gccttgagcg atacggaaac tgtcagcaga aatataagaa aaaatattat ttgtttcatc
     1441 agagggtaca aaaatagtgg catattcttt ttttgaggtg ctatgcaaaa tactttgata
     1501 gtctgaaaaa gaaaaaatcg gtgtttcgaa tacccttgtt tgaagctaaa aactttttaa
     1561 tttggccatt gaggttttca gcttttgcgt tggtttcccc attggtgaag tattcaacaa
     1621 tttcatcttc gttgctttca acgagtgatt taaagtttga taattcatca atatcagcat
     1681 catcaacatc ttcataccac tgatataaat ttttaagtgt ctgcaattga ttttgcccga
     1741 tattttcgtt tgaataccat gttctgaagc tgcaggttaa ataatatgcc gctttaattt
     1801 cagggaatac tctaaacatc aattccgctc ttttcatctg ccaaagtttc cactgatttg
     1861 gaaatttgta aagcaaaaaa tggcttctga ggagcaattc accaggcgtt tcgccgtttg
     1921 caagtctgtc ttctttgtaa atgaatttct ttggtgtaaa cttcagatgt tttttttcgc
     1981 aatcaataag ccgttgtttt tctgattctt taaactcctg atgggctttt cttctttgtt
     2041 caagaagttt ttgccggcat cggattctta cagcctgcat agcatcaagc aagttgttga
     2101 taacatgaaa tttgtctgcc acctgaatcg catttggcat gacttcctcg ctgaaccggg
     2161 catagcaacc agccatatca cgggttattc gtttcacttg tgataaacta tcgagtagcg
     2221 gctctacagc ttgtttcaag tagtctgatc ttgtcgtacc ggcacagaat gctattttcc
     2281 cggtttctct gtttgaaaga attgtgtaat actgctctcc aatcattttt tcatcaatgc
     2341 ccatgtttgc cccaatgttt tccggccgga gaataggaac ttctatattc gtttgcccat
     2401 gcgctgccat cacttcaatg tcgtgctcat ggatctgacc ggcaccatcg ggctcaaaac
     2461 cgctcaaatt attcttgtac cacttgtgta atgtttttgc atcaacaccc attatctctg
     2521 caaatacgga gatattcgtc aggtgtgtgt ccatgtactt cttttaaaaa agcagcaaat
     2581 tctttcgttg cttttatccc cggatgatta aattgatatg aattactata gtgttcatct
     2641 tccccggcta acttccatcg gcgccttttt attactaaat aagtcggctt cccttttatc
     2701 ggaaaactta ataacgacaa tggattgcaa aaaccatcca acaccgcatc aaaactctta
     2761 atctcttcag gaatcagatc cttcttctct tcaagtatta tttcaatctg aattgcgctc
     2821 tcatttactt ctgaaacatc gaaactttca agaatctcag acggaactat taagctgatt
     2881 attttgtcca ttcttttttt tgagctttta tacgtggcac ctcaaaaaaa gatatggcc
//
LOCUS       DAOPYB010000296         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_46892_length_2928_cov_7.390881, whole genome shotgun sequence.
ACCESSION   DAOPYB010000296 DAOPYB010000000
VERSION     DAOPYB010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46892_length_2928_cov_7.390881"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKH58_13285"
     CDS             complement(<1..436)
                     /locus_tag="PKH58_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HNX90043.1"
                     /translation="MKKRIAFIINPVSGTKNKAGLPGILRRELDPKQYDDYRILYTEY
                     KGHGMLLADQLVREGYHVVVAVGGDGTVNEVAKSLIHTNVALGIIPTGSGNGLSRHLK
                     IPMNVEKATQLLNKSEVIKADYGIVNEKPFFCTCGTGFDAYVS"
     gene            562..2700
                     /gene="rnr"
                     /locus_tag="PKH58_13290"
     CDS             562..2700
                     /gene="rnr"
                     /locus_tag="PKH58_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNX90044.1"
                     /translation="MSKRNKNTGSTRVRKSELVSNIADIFSGNQEKTYNYKQISTLLN
                     ITSESQRTFVNQLLYELADEDFLKEISRGKFRLNSRGGYITGTIDRHGVKTYLIPDDG
                     GEKIFISEQKTNTAMRNDKVKARLYAQRKGQEPEGEVLEIIQRAQDTFVGELDVSDNY
                     AFLIISNRIINNDIFIPKDKLKGGKSGQMAVVKLQEWKPNLKSPVGEVIDILGEKGNN
                     DTEMHAILAEFGLPYKYPENVEAEAAKIDAGITPEEVAKRIDMRGVTTITIDPRDAKD
                     FDDAISLRKLSNGLWEAGVHIADVTHYVRPGTIIDREGEERATSVYLVDRTVPMLPEH
                     LSNGICSLRPNEDKLTFSVLFQMNDQAEVLNYQIARTVTRSDRRFTYEEAQHIIETGE
                     GEFKEEILTLDKLAKQLREKRFKAGAIAFDRVEVRFDIDEKGKPLSVYFKEQKDANKL
                     VEEFMLLANKTVATHIGKPGKGLKAKTFVYRIHDVPDPDKLQNFSAFIRKFGYNLKAT
                     GKTTQISASINHLLDEVEGKKEQNLIETLAVRSMAKAIYSTQNIGHYGLAFDYYTHFT
                     SPIRRYPDMMVHRLLQRYLDGGRSVDTREYEDKCEHSSNREQLAANAERASIKYKQVE
                     FMSERVGQVFDGVISGITEWGIYVELIENKCEGMIPIRDLDDDFYTLDEKNYCLIGRR
                     FHKKYQLGDELTVKVAKANLERKQLDFVLA"
     gene            2848..2921
                     /locus_tag="PKH58_13295"
     tRNA            2848..2921
                     /locus_tag="PKH58_13295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2882..2884,aa:Arg,seq:acg)
BASE COUNT          909 a          674 c          639 g          706 t
ORIGIN      
        1 tactcacata ggcgtcaaaa cccgttccac aggtacagaa aaacggtttt tcgttgacga
       61 tgccatagtc tgcctttatc acttctgatt tattgagcag ttgagtggct ttctcaacat
      121 tcatgggaat tttcaggtgg cgcgacaacc cattgcccga accggttgga ataattccga
      181 gcgcaacatt tgtatgtatc agcgattttg ccacttcatt cactgttccg tcaccaccca
      241 cagccaccac aacatgatac ccttccctga ccagctgatc tgccagcaac attccgtgcc
      301 ctttgtattc ggtgtaaagt attcgataat catcgtattg tttagggtca agctcgcgac
      361 ggaggatgcc cggcaatccg gccttatttt tggttcctga taccgggttt atgataaaag
      421 caatacgttt tttcatgtga aatgaataaa tgatccggtg cgaaaataca tctaattttc
      481 ggaaaaagaa aaattatttt taatcagact gaaacataag ttctcagggc tttgttgtat
      541 atttgtcaaa aatccatcca tatgtcaaaa agaaacaaaa acaccggtag cacccgtgtt
      601 cggaaatcgg aacttgtcag taacattgcc gatatctttt ccggaaatca ggaaaaaacc
      661 tataactaca aacaaatttc caccctgctg aatataactt ccgagtcaca acgtaccttt
      721 gtcaatcagc tactgtatga actcgccgat gaggattttc tgaaagagat ttcacgtgga
      781 aaattcaggc ttaattcacg cggaggatac attacaggca ccatcgaccg ccacggagtg
      841 aaaacatatc ttataccaga tgacggaggt gaaaagattt tcatttcgga gcagaagacc
      901 aataccgcga tgcgtaacga caaggtgaaa gcccgtctct atgctcaacg aaaagggcag
      961 gaacccgaag gcgaagtgct cgaaataatc cagcgcgcgc aggatacatt tgtgggcgaa
     1021 ctcgatgttt ctgataatta tgcatttctg ataatcagta accggattat caacaacgat
     1081 atttttatcc cgaaagacaa actgaaaggg gggaaatcag gccagatggc agtagtaaaa
     1141 ctgcaggaat ggaaaccaaa tctgaaaagt ccggtaggtg aagtgattga tatactcggt
     1201 gaaaaaggca ataacgacac cgaaatgcac gccattctgg ctgaattcgg tttgccctac
     1261 aaatatcccg aaaacgtgga agccgaagca gccaaaatag atgccggtat cacacccgaa
     1321 gaggtagcca agcgaattga tatgcgcggc gtaacaacta tcaccatcga cccgcgcgat
     1381 gcaaaggatt ttgacgatgc catttcactc cgcaaactca gcaatggact ttgggaagcc
     1441 ggggtgcaca ttgccgatgt tacccattat gtccgtccgg gaaccatcat cgacagggaa
     1501 ggtgaagaac gtgccacttc ggtctatctt gttgaccgga ctgtacctat gctgcccgaa
     1561 catctttcga acggcatctg ctcactccgt cccaatgagg ataaactgac attctccgtt
     1621 ctgtttcaga tgaacgacca ggctgaagtc ctcaattatc agatagcacg tactgtaacc
     1681 cgcagcgacc gccggtttac ctatgaagaa gctcaacaca taatcgaaac aggcgaaggt
     1741 gaatttaaag aagaaatcct tacccttgac aaactggcaa aacagctgcg tgagaaacgt
     1801 tttaaagccg gagctattgc tttcgaccgg gtggaagttc ggtttgatat tgacgaaaaa
     1861 ggcaaaccac tcagtgttta tttcaaggaa caaaaagacg cgaacaaact ggtagaagaa
     1921 ttcatgctgc tggcgaataa aacagtagcg acccacatcg gaaaacccgg aaaaggtctt
     1981 aaggcaaaaa ccttcgtgta ccgcatccac gatgtacccg atcccgacaa attgcaaaac
     2041 ttctcagcat ttatccgtaa gttcggctac aatctgaaag caacaggcaa aaccacccag
     2101 atttcagcat ccatcaatca tttactggat gaagtggaag gcaaaaagga gcaaaatctg
     2161 atagaaaccc tggctgtgcg ctccatggca aaagctattt actccactca aaatatcggt
     2221 cactacggac tggcattcga ttattacacc cattttacct cacccatacg ccgctacccc
     2281 gacatgatgg tccatcggct gcttcagcgc tacctggacg gaggaagaag cgtggataca
     2341 cgtgaatacg aggataaatg cgaacacagt tcaaaccgcg aacaactggc cgccaatgcc
     2401 gaacgcgctt ccatcaaata caaacaggtg gaatttatgt ccgaaagagt tggacaggta
     2461 ttcgatggag tcatttcagg catcacggaa tggggtattt acgtggagct gatcgaaaac
     2521 aagtgcgaag ggatgattcc aatccgtgat ctcgatgacg atttctacac cctggacgag
     2581 aaaaactatt gcctgatcgg acgacgtttt cataaaaaat atcagttggg cgacgaactg
     2641 accgtaaagg ttgccaaagc aaatctggaa agaaaacagc tggattttgt actggcataa
     2701 cccattaaat ttccctctgg ggggggtact gaccaattct cctaaaatga aacataataa
     2761 ttactgtttt ttttatagaa atatttcgcg ataattattt gacaaatcaa aatattgcct
     2821 tatctttgca gtccgaaaca acggaatggc cccgtggctc aactgaatag agtatctgac
     2881 tacggatcag aaggttacag gtttgaatcc tgtcggggtc acttttac
//
LOCUS       DAOPYB010000297         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_47390_length_2907_cov_6.455470, whole genome shotgun sequence.
ACCESSION   DAOPYB010000297 DAOPYB010000000
VERSION     DAOPYB010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47390_length_2907_cov_6.455470"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /gene="hemW"
                     /locus_tag="PKH58_13300"
     CDS             complement(<1..1066)
                     /gene="hemW"
                     /locus_tag="PKH58_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNX90045.1"
                     /translation="MAGIYIHIPYCKTKCSYCDFFSGTNFGQQSELLDAMKAEIILQK
                     DYLGGAAVSTIYFGGGTPSTLSVNQIQEIMNSLRDNFQVEDCCEITLEANPEDLNITY
                     LNELRKTGINRLSMGIQSFDNEQLKSIRRRHTAETAVDAVKNAQTAGFNNISIDLIYG
                     LPYQSFIFWKYQIDKALTLNVQHISAYGLTYEQGTLLWKQLQKGEVVQVDDETMNAMY
                     AYLTEACTGNGFEQYEISNFAVPGYRSRHNSAYWKQQPYLGIGPSAHSYNIESRQWNV
                     ASISKYCNLIVTDRCWYEKELLSEQDKYNDFVMVSLRTVEGIDLKKSEAMFGKKLTEY
                     CLYSAEKYIEKKLLYVNNEFL"
     gene            complement(1072..2619)
                     /locus_tag="PKH58_13305"
     CDS             complement(1072..2619)
                     /locus_tag="PKH58_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90046.1"
                     /translation="MKVKILIFLFFVSGITGVSAKQIFVAVTGAITGTGTIVSPNDIV
                     TAISKASVGDTVYVRGGQYMNNTSLTFGKSGNSSFYLNIWAYADEMPVFDFRNQPYSN
                     SNPGIKLSGSYVYLKGIAVQGAGDNGMIVTGNNNRIEKCIFRWNCDSGLQLKTGSNNL
                     ILNCDSYENFDYQTGGIATPDYGGNADGFADKQYTNSGTNTYVGCRSWLNSDDGWDHY
                     EKIGNTVYDSCWCYANGPASYDVTEHIRFKTDSATWFYRFKNSSGRYVITNWGNGNGF
                     KLGGNYTAHNATLRNCVSVKNKEKGFDQNNDNGTMTLYNCTGYLNKRNYGFTNSSYGK
                     LVIKNCASLSSATSNSFTCKEVNQEYNTWNTGFSCTSADFVSLDYDQLLYPRQADGSL
                     PEINLLHLAATSGMIDKGINLGYAYRGKSPDLGAFEFNAQTAVIFPCKTEKLKVYFSN
                     TNKDIIVSGFASKIEIFDISAKKIFGIVENSENLSIPASDWAKGTYLIRVLQANTDLS
                     VYKIIIP"
BASE COUNT          838 a          605 c          521 g          943 t
ORIGIN      
        1 gtagaaattc attgtttacg tataaaagtt tcttttcaat gtacttttca gctgaatata
       61 agcagtattc agtcagtttt ttcccaaaca ttgcttctga tttttttaaa tcaatccctt
      121 cgacagtacg taatgaaacc attacaaagt cattgtattt atcctgctca ctgagtaact
      181 ccttttcata ccagcatcta tcagtaacaa tcaggttaca atatttcgat atggatgcca
      241 cattccattg tctcgattct atgttatagg aatgtgctga cggcccaatt ccaagatatg
      301 gctgctgctt ccagtaagcc gaattgtgac gcgaacgata acccggaaca gcgaaattgg
      361 atatttcata ctgttcaaat ccattgccgg tgcacgcttc agtcagatat gcgtacatcg
      421 cattcatggt ttcatcatca acctgaacga cctcaccttt ctggagttgt ttccatagca
      481 aggttccctg ttcgtaagtt aatccatagg ccgagatgtg ttgtacattt agcgtcaatg
      541 ctttgtcaat ctgatacttc cagaatataa acgattgata tggcaatcca taaatcaaat
      601 caatactgat attgttgaaa cctgctgttt gtgcattttt tactgcatcg accgccgttt
      661 cagccgtatg tcggcgacgt atggatttaa gctgctcgtt atcaaaactt tgaataccca
      721 tacttaaacg gtttatccca gtttttctca gttcattcag gtaagtaata ttcagatctt
      781 ccggatttgc ttcgagtgta atttcgcagc agtcttcaac ctgaaaatta tcccgtaaag
      841 aattcattat ttcctgaatt tgatttacgg atagggtaga aggtgtgcca ccgccaaaat
      901 aaattgttga aacagcagcg cctcccaaat aatctttctg aagaattatt tcagctttca
      961 ttgcgtccag caactcagat tgctgaccga agttggtgcc tgaaaaaaaa tcgcagtagc
     1021 tgcatttagt cttgcagtat ggaatatgaa tatagatacc agccatttct gtcagggaat
     1081 tataattttg taaacagaca gatcggtatt tgcttgaaga accctgatca gataggttcc
     1141 ttttgcccag tcagatgcag ggatggatag attctccgaa ttctcaacta ttccgaatat
     1201 ttttttagcc gagatgtcaa atatttcgat tttcgaagca aatccggaaa cgattatatc
     1261 tttgtttgta tttgaaaagt aaaccttcaa cttttctgtt ttacatggga aaatgaccgc
     1321 agtctgagca ttgaactcga aagctcccag atcaggagat ttgcccctgt aagcgtatcc
     1381 aagatttatc cctttgtcta tcattccact tgttgcagcc aaatgcaaaa ggttgatttc
     1441 cggaagtgaa ccgtcagcct gacgcgggta taacaactgg tcataatcca gactgacaaa
     1501 atcggctgaa gtacagctaa accccgtatt ccaggtatta tattcctgat taacctcctt
     1561 gcaggtaaaa ctgtttgacg ttgcactgct taatgaagca cagtttttaa tgactaattt
     1621 tccatagctg ctgttcgtaa aaccataatt tctcttgttt agataccctg tacaattata
     1681 taatgtcata gttccgttat cattattctg atcgaaacct ttttctttgt ttttaactga
     1741 tacgcagttg cgcaatgttg cattatgggc ggtataatta cctccaagct taaatccgtt
     1801 accatttccc caatttgtta ttacgtatct gcccgatgag tttttgaacc gataaaacca
     1861 ggtggcactg tcagttttga aacgaatatg ttccgtcaca tcgtatgatg ccggtccgtt
     1921 tgcataacac cagcagctgt cgtaaacggt attcccaatt ttttcgtaat gatcccaacc
     1981 atcgtcgcta ttcaaccacg aacgacatcc cacgtaagtg tttgttccgc tgtttgtgta
     2041 ttgtttgtct gcaaatccat ctgcattgcc accataatct ggagttgcaa tacctccggt
     2101 ctgatagtca aaattttcgt aagaatcgca gtttagtatc aggttattac tgccggtttt
     2161 tagttgtaat cccgagtcac aattccatct gaaaatgcat ttttcaattc gattgttgtt
     2221 tccggtcact atcatgccat tatctcctgc tccctgtacg gcaatacctt ttaaatacac
     2281 ataacttccc gaaagtttaa taccgggatt gctgttgctg tagggttgat ttctgaaatc
     2341 aaataccggc atctcgtctg cgtaagccca aatatttaaa tagaaagagg aattgccgct
     2401 tttaccgaaa gtcaaagagg tattgttcat atactgccct cctctgacat atactgtatc
     2461 accaaccgag gctttggata tagctgtaac aatatcattg ggcgatacta tcgttcctgt
     2521 accggttata gcgcctgtga cagctacgaa tatttgtttg gccgatacgc ctgtaatacc
     2581 cgacacaaaa aagagaaata ttagtatctt aactttcata tttttcagat gattacatgc
     2641 tcagaaatgc aaaaataaca actattagtc atttagttcc gatttacggt ttattagaga
     2701 ttgtattaat taattagtta gtaatacatt agaaccaatt agcagtactt cgtagaaata
     2761 attctgcttt aaagaacaaa gatattggaa aaataatcaa aaggtaatta ttatggttta
     2821 aaatgaaaaa agatttaccg aaaatcgata aatcttttca agtcggggta gcgggattcg
     2881 aacccacgac cccctgctcc caaagca
//
LOCUS       DAOPYB010000298         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_47866_length_2889_cov_14.205363, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000298 DAOPYB010000000
VERSION     DAOPYB010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47866_length_2889_cov_14.205363"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PKH58_13310"
     CDS             <1..1239
                     /locus_tag="PKH58_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNX90047.1"
                     /translation="QRIIEWLVQEFKNDNSGVDLSKDPMALQRLKEAAEKAKIELSNT
                     TSTEINLPYIMPVDGVPRHLVRTLTRAKFEQLIDDLIQRTIEPCRTALKNANLTINDI
                     NEVILVGGSTRIPAIQSAVEKFFGKAPSKGVNPDEVVAVGAAIQGGVLSGDVKDVLLL
                     DVTPLSLGIETMGGVMTKLIEANTTIPTKKSETFTTAADNQPSVEIHVLQGERPMAAH
                     NKSLGRFHLDGIMPARRGEPQIEVTFDIDANGILHVSAKDKATGKTQSIRIEASSGLS
                     DAEIKRMKDEAAANAAADAQEKERIDKMNHADSLIFQTEKQLSEIGDKIPADKKGPIE
                     AALVKLKTAKNAQDVAGIDAATNELNNAFQAASQEMYNAQNAQAGGQQAGPQDFGGQQ
                     PGSSDNNQGDVTDVDFEEVK"
     gene            complement(1418..>2889)
                     /locus_tag="PKH58_13315"
     CDS             complement(1418..>2889)
                     /locus_tag="PKH58_13315"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNX90048.1"
                     /translation="NNCFVDWLSDTTNISIAAFCPEDNNLPTAESIRSQFINRKQIEV
                     EPYTDNSPTGKLVDDKLFTRISRVKKEQKGKFGSTVWTLSNGARIILKPTDLKENQIR
                     LTVISPGGTSLYDDSEIKNLELMSSVATLGGYGDYNKIQLRKLLSGKSTNVVPTVATL
                     REGIVGYTTPKDFETMMQLVYLQFTSPRKDSIAYNSFMNRYRTQLEGKEKDPSSDLSD
                     TLLVAMYDRHPRAISLKPDDLNKIDYDRSMKIFKERFSDVSDFTFYLVGDFKPDSIKP
                     VIEKYIGALPASQSNEKYVDRKKYIRKGNYENNFTRKMETPKSTVVAILHAPEKANLK
                     NEIQMDMLTQILRMIYTEEIREKEGGTYGVSVKGAVVRIPKQLVNIQISFATNPERRD
                     HLTKLVLDLYRNFTEKGPSPEMVDKVRQYMLKKHKEELIDNSYWMDMLTSYYWEKEDI
                     HSSYEKILKETSPKDIQRFAKKILDSGNLIQVSMTGVNE"
BASE COUNT          842 a          591 c          591 g          865 t
ORIGIN      
        1 cagcggatta tcgaatggct ggtacaggaa ttcaagaatg ataacagcgg cgtagatctg
       61 agcaaagatc cgatggcgtt gcaacgtttg aaagaagctg ctgaaaaagc aaaaatagaa
      121 ctttcaaata caacttctac cgaaatcaat ctgccgtata tcatgccggt tgacggtgtg
      181 ccacgccacc tggttcgtac acttacacgt gcaaaattcg aacagttgat cgacgacctg
      241 attcaacgta ccattgaacc ttgccgtact gcattgaaaa atgccaatct gacaatcaat
      301 gatatcaacg aagttatcct tgtaggtggt tcaacccgta ttccggctat ccagtcagcc
      361 gttgaaaaat tcttcggaaa agctccttca aaaggtgtta accccgacga agttgtagcc
      421 gtgggtgcag caatccaggg tggtgtgctg agtggcgatg tgaaagatgt attgttgctc
      481 gacgtaactc cgctttcact tggtatcgaa acaatgggtg gagtgatgac caaactcatc
      541 gaagccaaca caaccatacc gactaaaaaa tcggaaacat ttacaacggc agcagacaat
      601 cagccttcgg tggaaattca tgtattgcag ggcgaacgtc cgatggcagc acataacaaa
      661 tcgctgggcc gtttccacct cgatggaata atgcctgcgc gcagaggcga accgcagatt
      721 gaagttactt ttgatattga tgcaaacggt atacttcatg tatcagctaa agataaggct
      781 accggaaaaa cccagagtat ccgtattgaa gcttcatccg gattgagcga tgctgaaatt
      841 aaacgcatga aagacgaggc tgcagcaaat gctgccgctg atgctcagga aaaagaacgc
      901 attgataaaa tgaaccatgc cgacagtctt attttccaga ctgaaaagca gttgagtgaa
      961 atcggtgata aaatccccgc cgacaagaaa ggtccgattg aagctgcgct cgttaaactg
     1021 aaaactgcca agaacgctca ggatgttgcc ggtatcgacg cggctaccaa cgaactgaac
     1081 aatgcattcc aggctgcaag ccaggaaatg tataacgccc agaatgctca ggcaggcgga
     1141 caacaagcag gtcctcagga ctttggcgga caacagcccg gtagcagcga caacaatcag
     1201 ggcgatgtaa cggatgtaga ctttgaagag gtaaaataaa atccagaaac aaggattaat
     1261 ttctataaat aaaaaccgct gtaaatgtac aatttacagc ggtttttatt ttgttttctc
     1321 ccgatgagaa tatatctcaa tgttattcga aacattgccg gtatactctg gaagcttatc
     1381 ttgttatgcg tgtaataaga taagtactta aacttattta ttcgtttact ccggtcatgc
     1441 tgacttgaat taaattacct gaatccaaaa tcttcttagc aaaacgctga atgtctttag
     1501 gtgaggtttc cttaagtatt ttctcataac tagaatgtat gtcttctttt tcccagtaat
     1561 aggaagttag catatccatc cagtaactat tatcgattaa ttcctcttta tgtttcttga
     1621 gcatatattg ccgtacttta tcaaccattt caggcgaagg acctttttcg gtaaaatttc
     1681 tatacagatc taataccagt tttgttaaat gatcacgacg ttcaggattc gtagcgaatg
     1741 agatttgaat attaaccagt tgtttcggga tacgaactac cgctcctttt accgaaacac
     1801 cataagtccc gccttctttt tcccttattt cttcagtata aatcattcgc aggatttgcg
     1861 tcagcatatc catttgtatt tcatttttta agtttgcttt ttccggagca tgaagaatgg
     1921 caactactgt tgatttggga gtttccattt ttcgcgtgaa attattttca taattccctt
     1981 tgcgtatgta tttttttcta tccacatatt tttcattgct ttgagatgct ggcaaagccc
     2041 ctatatactt ttcaataaca ggttttatgc tatccggttt gaaatctccg actaaataaa
     2101 atgtaaaatc actgacatcg ctgaagcgtt ccttgaaaat tttcatactc cggtcgtagt
     2161 ctatcttatt taaatcatca ggttttaatg atatagcgcg gggatgacga tcatacattg
     2221 caacaagaag tgtatcagaa agatccgagg aagggtcttt ttcttttcct tccagttgag
     2281 tcctgtaacg attcatgaaa gaattgtaag caatggaatc ttttcttgga gatgtaaatt
     2341 gtaaataaac aagttgcatc atcgtttcaa agtcttttgg tgtagtataa ccgacaatgc
     2401 cttcccggag tgttgcaaca gttggtacta cattcgtact tttaccggaa agcagttttc
     2461 ttagctgtat tttattgtaa tcaccataac cacccagagt agctacgcta ctcattaatt
     2521 cgagattctt tatttcactg tcgtcatata aggatgttcc accaggactt attacagtca
     2581 gtcttatttg gttttccttc aggtctgttg gtttcagaat gatgcgggcg ccatttgaaa
     2641 gtgtccatac agttgaaccg aatttgcctt tctgctcttt ctttactctg cttatccggg
     2701 taaataattt atcgtccact aattttccgg taggggagtt gtccgtatat ggttcaactt
     2761 caatctgttt gcgattgatg aattggctac gtatgctctc cgcggttggt aaattattat
     2821 cttccggaca aaaggcagca atagaaatgt tggttgtgtc agacaaccag tcaacaaaac
     2881 agttattaa
//
LOCUS       DAOPYB010000299         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_47867_length_2889_cov_12.803458, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000299 DAOPYB010000000
VERSION     DAOPYB010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47867_length_2889_cov_12.803458"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKH58_13320"
     CDS             <1..665
                     /locus_tag="PKH58_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90049.1"
                     /translation="SPYMYCGGNPVRLVDVDGREWLEPIDVEKCNELRKRAVAERGNY
                     KEGSDEYNRIQEGIDGLDFMTQDKERKYTFTELYKDSYDTESTTAGYVKREGKDPANG
                     TYHICYRDDNNFSNFSDSEMGKAWHETVHLTRMLEEHDIPYREWAKTGGRQSNDGIIG
                     YLMGPNDRLSCCTENIHHEEYAAYRSQQLVFPNSMPTPVGCVFGSIKTDTELNQYIKN
                     NY"
     gene            670..1572
                     /locus_tag="PKH58_13325"
     CDS             670..1572
                     /locus_tag="PKH58_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90050.1"
                     /translation="MKQIQSLYIIVFICITMSYCLYGQGQVQDKRNQETYYWKNQSIS
                     FFPDGTYELKQLPSFVLLPNIPVLSYGKYIRYKKYAYYLFSDTTYDCWHRSAIQLTGT
                     ESHSPDSNYIIDIVDTVNEHDSCFIIDYLTRKEVVLRFHYYNVRVYYDRFAFFDYRKS
                     NPTFNFHEKGIHQDAPLDFYIELTTHDNKIEFQNPLQLPIGKIKIKIMGAPMEYFWEY
                     VVQDTSANNFVINIPERVYLRLYFQEFNSDVVIIIDKKSIGFSGHIWQKKDEAFDYYM
                     KANTRQRKIWSRYWTLPDPYYKGK"
     gene            1697..2689
                     /locus_tag="PKH58_13330"
     CDS             1697..2689
                     /locus_tag="PKH58_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNX90051.1"
                     /translation="MKSTKNDKIKEINITPAHGRSRGKTTAQYVQHLCYLPFGESFVD
                     QRVTNYEGSRYTFSGKERDSETGYSYFGARYYSSDYSIWLSVDPLSDKYPSMSPYMYC
                     GGNPVRLVDVDGRDWVDIYGNKIKDHSNIKVYIFYDPNSFSHQSTEMAQNVIDKYGAG
                     SVALSNVTTASEFSNDWKSMASPTMEVVNLNYHGDNQTLVLNQSTKEFITSTGNGLTN
                     ENSYGAMNVQALPIPAGNISKAQLNINSCNSNNMEQYELKGSRLTLMRAFYRTFSFET
                     VRGASVGVSYDRWTHEPEPQWFWQSWDYMGKIPETPKLSLDCSDAFYFKGGGHK"
BASE COUNT          952 a          506 c          595 g          836 t
ORIGIN      
        1 tgtcgccgta catgtattgc ggggggaatc cggtgcggct ggtggatgtg gatggaagag
       61 agtggttgga acctattgat gtagaaaaat gcaacgaact tcggaagcga gctgtcgcag
      121 aacgtggtaa ttataaagaa ggctcagacg aatataatag gattcaagaa ggtattgacg
      181 gcttggattt tatgactcaa gataaagaac gcaaatatac ttttacagaa ctctacaagg
      241 attcatacga tactgaaagc actacggcgg ggtatgtgaa acgcgaaggg aaggatccgg
      301 caaatgggac ctatcatatc tgttatcgtg acgataataa tttttcaaac ttttcagact
      361 ctgaaatggg aaaggcatgg catgagactg ttcacctgac aagaatgcta gaagaacatg
      421 acatcccata tagagaatgg gcgaagacag gcggaagaca atccaatgat ggaatcatag
      481 gttatcttat gggtccaaat gatcgattga gctgctgtac tgaaaatatt caccatgaag
      541 agtatgccgc ttacaggtcc caacaattgg tttttcccaa ttcgatgcct acgccagtag
      601 gatgtgtttt tggatctatt aaaacagata cagaattgaa tcagtatatt aagaataatt
      661 actaaatcta tgaaacaaat tcaaagccta tatataattg tatttatttg cattacaatg
      721 agttattgct tgtatggaca aggtcaagta caggataaaa ggaatcagga aacatattat
      781 tggaaaaacc aatcaatttc attctttccg gacgggacat acgagttaaa acaattacca
      841 tcgtttgtat tattgccaaa cattcctgtt ttgagttatg gtaaatatat ccgatataaa
      901 aaatacgcat actatctgtt tagtgataca acctacgatt gctggcacag atcagccata
      961 caattgacag gaacggagag ccattctcct gactcaaact acataattga cattgtggat
     1021 acagttaatg aacacgactc ttgtttcatt attgattacc ttaccaggaa ggaagttgta
     1081 ctaagattcc attattataa tgttcgtgta tattatgaca ggtttgcttt ttttgattat
     1141 cggaaaagca atcccacatt taatttccat gaaaagggga ttcaccagga cgccccgttg
     1201 gatttttaca tagaattaac cacccacgat aacaaaattg agtttcaaaa tccgctacaa
     1261 ctccctatcg gaaaaatcaa aataaagatt atgggtgcgc ctatggaata tttttgggag
     1321 tatgttgttc aggatacttc tgccaataat tttgttatta acattcctga aagagtatat
     1381 ctccgacttt actttcaaga attcaacagt gacgttgtta ttattattga taagaaatca
     1441 attggttttt caggacatat ttggcaaaaa aaagacgaag cattcgatta ttatatgaaa
     1501 gctaatacaa ggcaaagaaa aatttggtcg cgctattgga cgttaccaga tccctattat
     1561 aaagggaaat agaaatgtca caataacagt atctactatc aacaaaagca ctttataaac
     1621 aaatatatac tattatgctc ccccaatata aaccaacacc aataattcct atctttgccc
     1681 tcaaattcgt ttcgctatga aaagcacaaa aaacgataaa attaaagaaa taaacatcac
     1741 tccggcgcac gggcgttcgc gcgggaaaac caccgcacag tacgtacagc acttgtgtta
     1801 cttgcccttt ggcgaaagtt ttgttgacca acgcgttact aactacgaag gttcgcgcta
     1861 caccttctcc ggcaaagaac gcgacagcga aacgggttat agttacttcg gcgcgcggta
     1921 ctattcttcg gattacagca tctggctgag cgtggacccg ctctcggaca agtacccgag
     1981 tatgtcgccc tacatgtatt gtggggggaa tccggtgcgg ctggtggatg tggatgggag
     2041 agattgggta gatatctatg gaaataaaat aaaagaccat agtaatatca aagtttatat
     2101 attttatgat cccaattctt tttctcacca atccacggag atggcacaga atgttataga
     2161 taaatatgga gctggtagtg tcgcattaag taatgttacc accgctagtg aattttctaa
     2221 tgattggaag agtatggcga gcccaaccat ggaggtagtc aatttaaact atcatgggga
     2281 taaccaaact ttggttctaa atcaatcaac aaaagagttt attacatcaa ctggaaatgg
     2341 tctgacaaat gaaaatagtt atggagccat gaatgtacaa gctcttccta taccggcagg
     2401 caatatttct aaagctcaat tgaacataaa ttcctgtaat tcgaataata tggaacaata
     2461 tgaactgaaa ggttcacgat taactctcat gagggcattc tacaggactt tttcatttga
     2521 gacagttaga ggggcatcag taggtgttag ttatgaccga tggactcatg aacctgaacc
     2581 gcagtggttt tggcagagtt gggattatat gggaaaaatc cccgaaacac ctaagttaag
     2641 ccttgattgt agtgatgctt tctattttaa aggaggaggg cataaataat gaaacgttgt
     2701 tatattttag ttatcatatt ggtattcctg tcaattataa tgtgtagcat atattatttt
     2761 gtccctcgtc acgttgattt tgaactcatt aaaactattg agcagcccga tcccttttac
     2821 gatagaactg aatgcaaagg ttttgattat atctatagtc aagatgattt atggttttgg
     2881 ttaagagaa
//
LOCUS       DAOPYB010000300         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_48933_length_2849_cov_12.352183, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000300 DAOPYB010000000
VERSION     DAOPYB010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48933_length_2849_cov_12.352183"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKH58_13335"
     CDS             <1..275
                     /locus_tag="PKH58_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNX90052.1"
                     /translation="ESIEGRTVIIIEDIVDTGHTLEKLYTELQKKNPAKIMIATLLYK
                     PESYKGSHKVDYVGKSIPNDFIVGYGLDYDGIGRNLPDIYTLARQL"
     gene            299..880
                     /locus_tag="PKH58_13340"
     CDS             299..880
                     /locus_tag="PKH58_13340"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNX90053.1"
                     /translation="MLNIVLFGPPGAGKGTQSEKLIAKYKLQHLSTGDILRAAIQNQT
                     PLGIEAKKFIDKGELVPDETVIGLIAKELDDHKDVKGFIFDGFPRTTHQAQELDKMLT
                     SKGISITLMMALEVEHQELVKRLLNRGQLSARSDDRNVDIIENRIKVYNEYTQVVADY
                     YKAQDKYRTVHGMGKIDEIFERLCHTIKEFQKD"
     gene            1190..2185
                     /gene="obgE"
                     /locus_tag="PKH58_13345"
     CDS             1190..2185
                     /gene="obgE"
                     /locus_tag="PKH58_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNX90054.1"
                     /translation="MASSNFVDYVKIYCRSGKGGAGSVHFRREKFIPKGGPDGGDGGR
                     GGHIYLKGNNQLWTLLHLKYKKHIIAESGQSGSGARCSGSDGKDITIEVPLGTVARNA
                     ETNEVICEITEQNEQVMLMKGGRGGQGNWNFKTATLQTPRFAQPGEPEIEAHIILELK
                     ILADIGLVGFPNAGKSTLLSVVSAAKPKIADYPFTTLVPNIGIVQYRDNRSFVMADIP
                     GIIEGAHEGKGLGLRFLRHIERNSMLLFMVPADSEDVNAQYDILINELKMFNPELLDK
                     KRVLAITKSDLIDAKIQKKIEKKLPDVPHIFISSQTNKGIQELKDLLWLQMNNEG"
     gene            2417..2845
                     /locus_tag="PKH58_13350"
     CDS             2417..2845
                     /locus_tag="PKH58_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX90055.1"
                     /translation="MIYRKHGIKTLWWLLAIAIVVAAADQIASGILKNGFQRLRPCHN
                     PDLQGLIHLVGRCGGKFGFASSHAANTFGVAVFLSFLYNNRWATLGLLFWAAFVSYSR
                     IYLGVHYPGDVICGAIIGAGVGSGVWFLANRFINKQEKSI"
BASE COUNT          865 a          566 c          619 g          799 t
ORIGIN      
        1 aggagagtat tgagggaaga actgttataa ttatcgaaga tattgttgac acagggcata
       61 cactcgaaaa actttacact gagcttcaaa agaaaaaccc tgccaaaata atgattgcaa
      121 cacttttgta taaacctgaa tcatacaagg gtagccataa ggtagactac gttggaaaat
      181 cgattccaaa tgattttatt gtagggtatg ggcttgatta cgatggaata ggtagaaatt
      241 tacccgacat ttatacttta gctagacaac tatagtaagt taacctaaaa ttatccctat
      301 gctaaacatt gtcctatttg gtcctccggg tgcaggtaaa ggtacacaat cagagaagct
      361 tattgctaaa tacaaacttc agcacctttc aacaggtgat attttgcgtg ctgcgattca
      421 gaaccaaacc ccacttggaa tagaagccaa aaagtttatc gataaaggcg agttagtccc
      481 cgacgaaaca gttattggtc ttatcgcaaa agagcttgat gaccataagg atgttaaagg
      541 ttttattttt gatggcttcc cacgcacaac acatcaagcg caggagctcg acaagatgct
      601 tacgagcaaa ggtatctcaa tcactttgat gatggctctt gaggttgaac atcaagaact
      661 tgtaaaacgc ctactgaaca gaggacagct atcggctcgt tccgacgatc ggaatgttga
      721 tattatcgag aatcgcataa aggtttacaa cgagtacacc caagttgtag ccgattacta
      781 caaggcacaa gacaagtatc gaacggttca cggaatggga aaaatcgatg aaatattcga
      841 aagactttgc catactatta aagagtttca aaaagactaa aagaatggaa gtatggaata
      901 atggagcatt gggataatta acatttatct tttcttccca ttcatacata atttcatcta
      961 attgattcaa aacaataata aattaaataa gtagccacaa acagtcctaa acattagaga
     1021 tttttacata acattccgca cttgatgcgg aatccatcaa ttttacttat ggatttctgc
     1081 attgcctttg gcgagtttgc gagctcggtt cgcaggaatg acttgtgaaa cgcattattt
     1141 taggtgtgga attggtcaat caacttaata atatttacaa catacatcca tggcatcatc
     1201 taactttgtc gattacgtaa aaatctactg ccgctcgggg aaaggtggtg caggtagcgt
     1261 tcacttccgc agggagaagt ttatccccaa gggtggtccc gatggtggcg atggtggccg
     1321 tggtgggcac atctacctta agggcaacaa ccagctgtgg acccttctgc atctcaaata
     1381 caaaaagcac attattgccg aaagtggtca atcgggtagc ggtgcgcgct gctcaggttc
     1441 cgacggtaag gatattacca ttgaggtacc tttgggaacg gttgcccgga atgctgaaac
     1501 caacgaggtg atctgcgaga ttactgagca aaacgagcag gtaatgctta tgaagggtgg
     1561 tcgtggcggc caagggaact ggaacttcaa aactgcaacc ctgcaaaccc ctaggtttgc
     1621 acaacccggc gaaccagaaa ttgaagcgca catcatcctt gagctaaaaa tccttgctga
     1681 tattggactt gttgggtttc ctaatgctgg taaatcaacc ttactatcgg ttgtttcggc
     1741 ggctaagcca aaaattgccg attacccatt tacaacgctg gttcctaaca ttggcattgt
     1801 tcaatatcgc gacaatcgtt cgttcgtaat ggctgatatc cctggaatca ttgaaggtgc
     1861 acacgaaggg aaagggcttg ggttaaggtt tttgaggcat attgagcgta actctatgct
     1921 cctattcatg gttcctgccg atagcgaaga tgtgaatgct cagtacgata ttcttatcaa
     1981 tgagcttaaa atgttcaacc cagagctgtt agataaaaaa agggtgcttg ccatcaccaa
     2041 atccgacctg attgatgcta agatccaaaa gaagatagaa aagaagctgc ctgatgttcc
     2101 acacatcttt atctcatcgc aaaccaataa agggattcag gagctgaagg atttgctttg
     2161 gctccagatg aataatgagg gatagaaaat cagaatacag tagccagtag taaggagtaa
     2221 ggagaaagta tattactact gaagtctact aattactgtc tacttaaata ctacaaaaat
     2281 gctccaaacg cttctacact gggacaccca actattccta ttccttaacg ggttacactc
     2341 ccctttctgg gattccatca tggtttttgc atcaggaaag ttcacttgga ttcttcttta
     2401 cctattactt gtctatttga tttaccgtaa gcatggaata aaaacccttt ggtggctttt
     2461 ggcaattgcc attgtagtag cggctgccga tcaaattgcc tcaggtattc ttaaaaatgg
     2521 atttcaacgt cttcgtccat gccacaatcc cgatttgcaa gggttgattc accttgtagg
     2581 cagatgcggt ggaaagtttg gctttgcatc atcgcacgca gcaaacacat ttggggttgc
     2641 agtatttctc tcgttcctat ataataatcg atgggctaca ttagggttac tattttgggc
     2701 tgcttttgta tcgtacagcc gcatttatct tggtgtacat taccccggcg atgtaatctg
     2761 cggagcaatt attggtgcag gtgtaggaag tggtgtttgg ttccttgcca atcgtttcat
     2821 taacaaacaa gaaaaaagta tttagttta
//
LOCUS       DAOPYB010000301         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_49201_length_2839_cov_8.594828, whole genome shotgun sequence.
ACCESSION   DAOPYB010000301 DAOPYB010000000
VERSION     DAOPYB010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49201_length_2839_cov_8.594828"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..526)
                     /locus_tag="PKH58_13355"
     CDS             complement(143..526)
                     /locus_tag="PKH58_13355"
                     /inference="COORDINATES: protein motif:HMM:NF023021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3244 domain-containing protein"
                     /protein_id="HNX90056.1"
                     /translation="MKNLIFRLILMVFILGLSSGTLRAGDITYEQEIDLRKQNPSGPP
                     RAPSIYPVSFSASLFMDEITIGSQNYTGDITVQITGISGSIIQTLQISASTTVVVDVS
                     ALPEGVYNIAIVTVGKGTFKGEFQK"
     gene            complement(715..2364)
                     /locus_tag="PKH58_13360"
     CDS             complement(715..2364)
                     /locus_tag="PKH58_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90057.1"
                     /translation="MMTKRMFLYIIALTIVLLFAACNRQRNEADRMRLNKLDSLLSIQ
                     PEAAADSLKQFYPARLSHYNRGYYQLLEVIALDKTYYNFTSDSLISSTVHILSRHKQT
                     YPHTYARSLMYQGLVRYRMGVTDSTAYEPIKEAADLLETKKINDPLLLYFCYHYIGLI
                     HYENNNPSYSIMYYEKAIKKIKQYGDKNYLFNTYFEITWAYLKVRKFNVAKQYIDILC
                     NFKNISDTQKACINQILSVYYEFTNQASKALEINKQLLRSNYSYTDMSSMLFKISNNY
                     KTLNKLDSALLYAKKAEECKPDSDYYLNNLYYKNIGEISEQLMLWHISANAYKTAYTL
                     QEKATSKALDKQIMTLEKKYDLKEAENKALQLTNRLIFTVFVSLILLILLVTSTVIFR
                     QRGKHAEIKTRLLAHEKINVERNLIEKEFMLPLYQQISQRNANIKTFLSDLLTNPHLT
                     KNPQLLQKIDETYQDFIKTSHINEFLTDEKFTEFTGMECESYQFLTENEKMLLVFAAM
                     KLDNRQIAVLFNTTESSVRGRKAKLRVKLESNNIDIKSIII"
BASE COUNT          860 a          507 c          516 g          956 t
ORIGIN      
        1 ataaaagatc ttttgccaga tgaattttgg aagttaagtt cgacgtagcg aaagctacgc
       61 cgaacatttt tgaccattta cctacttact aaaggatacc gggtgataaa aagatagctg
      121 actttgaaaa tctcaaaaga gattactttt gaaattctcc cttaaatgta cccttaccaa
      181 cggtaacaat agcaatatta tacactcctt caggcagagc gctaacatca actacaacag
      241 ttgtggatgc agaaatctga agtgtttgaa tgatgctgcc acttataccc gtaatctgta
      301 cggtaatatc accagtatag ttttgtgaac caatggttat ctcgtccata aaaagtgagg
      361 cggagaagct taccggatag atggacggtg cacgtggagg accagaagga ttctgttttc
      421 tcaaatcaat ttcctgttcg taggttatat ctcctgcacg gagcgtaccg gatgatagtc
      481 ccaaaataaa taccatcaag ataaggcgaa aaattagatt tttcatgttg taattttttt
      541 agagtttata ttgtaattgt ttttttgaat ttatgtttgc taaattacct gaaacaaaaa
      601 actcctgcaa gacttttgcg ggagttaaat gaaagtaacg cgaaaaacga gcgtaataca
      661 ttatttatca attgcataaa aaataaaagt aacgcatgat cagacaaaca atttttagat
      721 tataatgctt tttatgtcaa tattattgct ttcaagcttc accctaagct tcgcttttcg
      781 tccgcgtacg ctcgattccg tcgtattaaa aagcactgcg atctgtcggt tgtcaagttt
      841 catggcagca aaaacaagca gcatcttttc gttttcagta agaaattgat aactttcaca
      901 ttccattccg gtaaattcgg tgaatttttc atctgtcagg aattcattaa tatgcgatgt
      961 ttttataaaa tcctgatagg tttcatcgat tttttgtaaa agctgcggat tttttgtaag
     1021 atgcggattg gtcaataaat cggataaaaa tgttttaatg ttggcattgc gttgggatat
     1081 ttgctgatac aaaggcagca taaactcttt ttcgatcaaa ttcctttcta catttatctt
     1141 ttcatgcgcc agcagtcgtg tttttatttc ggcgtgtttt ccgcgttgac gaaaaataac
     1201 agtggaagta acaagcaata ttagtaaaat caaactaaca aagactgtga atatcaaccg
     1261 gtttgtgagt tgaagcgcct tgttttccgc ctcctttaaa tcgtatttct tttccagcgt
     1321 cataatttgt ttatcaagcg ctttggatgt agctttttct tgaagagtat aagctgtttt
     1381 atatgcattt gcacttatat gccataacat aagctgttca gaaatttcac caatattttt
     1441 ataatacaaa ttattaagat aataatccga gtcgggttta cactcttctg cttttttagc
     1501 atatagcaat gcgctgtcta atttatttaa tgtcttgtaa ttatttgaaa tcttaaataa
     1561 catgctggac atgtcagtgt aactgtaatt ggaccttaaa agttgcttgt tgatttcaag
     1621 tgccttcgag gcttgattag taaactcgta atatactgat agaatttggt taatacacgc
     1681 cttttgtgta tccgaaatat ttttgaaatt acataaaata tcaatatact gttttgcaac
     1741 gttaaatttt cgtactttta aataggccca ggtaatttca aaataggtgt taaacaggta
     1801 atttttatca ccgtattgct ttatcttttt aatcgctttc tcataataca tgatactgta
     1861 tgatggatta ttgttttcat aatgaattaa tccaatataa tggtagcaaa agtacaagag
     1921 cagcggatca tttatttttt ttgtttccag taaatcagcg gcttctttta tcggttcata
     1981 ggctgtacta tcggtcactc ccatgcggta acgcaccagt ccctgataca ttagcgagcg
     2041 tgcataggtg tgcggatacg tctgcttatg gcgggatagg atgtgtacag ttgaactgat
     2101 caggctgtcc gatgtgaaat tgtaataggt tttatccagt gcaatcacct caaggagctg
     2161 ataataaccg cggttgtaat gtgagagtct ggcgggatag aattgtttca aactgtctgc
     2221 tgccgcttcg ggctgtatgg ataataaact gtctaactta ttgaggcgca ttcggtcggc
     2281 ttcgtttcgc tgtctattgc atgccgcgaa caacaacact atggttaatg caattatata
     2341 gagaaacatt cgtttggtca tcatgctgga ttttttctgt ttttaaaccc gcaaaaatag
     2401 cagttaaaat ccaaaaaaca agaataaatc gcaaaaaact gacacaattt ctgtacaaat
     2461 gagcaattgc taagcactta aagcgatgtc ccggtttatt actttttcaa tcctgagtcg
     2521 tgataataac accggttcaa cagcatgatt aaacatattc ttttcaaccg ttttgagata
     2581 cattagaagg aacatgtgta attactcttt ttacttgtca gcgggtgaaa tgaaacttga
     2641 gtaattagta tttaaccctt ttcatcgcaa tattccctac tttcagttta ataaagctta
     2701 tccgtaagct tgaaaacatt catttaacta ctacgaccca aatatggctg accctacttc
     2761 agaataatca acctgtgata atcaggaatg gaagattacc gtgtccttca catcccgcct
     2821 cttcaaccaa ccgcctgtt
//
LOCUS       DAOPYB010000302         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_49292_length_2835_cov_11.624820, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000302 DAOPYB010000000
VERSION     DAOPYB010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49292_length_2835_cov_11.624820"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PKH58_13365"
     CDS             <1..948
                     /locus_tag="PKH58_13365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNX90058.1"
                     /translation="QMYVKKYFPAKSKERMVQLVKNLQTALGERINGLEWMGDSTKAK
                     AQEKLKTFHIKVGYPDKWRDYTSLDIQKDSYWANIKRANKFESDYMFAKIGKPVDKDE
                     WLMTPQTVNAYYNPSTNEICFPAGILQYPFFDMQADDAFNYGSIGVVIGHEMTHGFDD
                     QGRQFDKDGNLKDWWTKEDGEKFNARAKVLSDYFDKIEVAPNVKANGKFTLGENIADH
                     GGLQVSYHALQNVLKTSPLETKDGFTPEQRFFLAYANVWAGNIRPEEILKRTKSDPHS
                     LGKWRVNGALPHIQAWYDAFNVKQTNKMYVAPELRASIW"
     gene            complement(1017..1196)
                     /locus_tag="PKH58_13370"
     CDS             complement(1017..1196)
                     /locus_tag="PKH58_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90059.1"
                     /translation="MKPKKKKINPIKDVLKAARKESREDEIKAHGKPVLHTKVAQPKN
                     KYNRGKNKKIDIPDD"
     gene            complement(1203..1637)
                     /gene="tsaE"
                     /locus_tag="PKH58_13375"
     CDS             complement(1203..1637)
                     /gene="tsaE"
                     /locus_tag="PKH58_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNX90060.1"
                     /translation="MMTILEIKSIQTIKFTAKQFLTYLKSNKETVYCFNGKMGAGKTT
                     FIKAICEEMGVKETVNSPTFSIVNEYEASDGSIIYHFDCYRINKIQEALDLGAEEYLY
                     SGNYCFIEWSENIASLLPDNTVNVDIEETEKGIRMIVISPLN"
     gene            complement(1737..>2835)
                     /locus_tag="PKH58_13380"
     CDS             complement(1737..>2835)
                     /locus_tag="PKH58_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX90061.1"
                     /translation="ENYKSKSIIANSVVNNTDVFAYAEDENAAYVNILRVSKGAIIQG
                     YTIEYKKRIDETKEDILSIAIIELREKLNSNSKEIIIPFPIEIEFAYIKFIVPRRGDR
                     KKLLELSEQNVKQYKLEKLKQTAKLNPDQKSIQLLTEIQQKLHLEKIPITIEAFDNSN
                     ISGSDAVAACVVFKKAKPSKKDYRKYIIKTVQGPDDYASMKEVVKRRYIRMVNENAPL
                     PDLIIADGGIGQMEVIRQVVEDELNLKIPIAGLAKNDKHKTSELLYGFPPKEIGMKPN
                     DTLFKLLAFIQEEVHRFAITFHRDKRSKNQTASELDNIRGIGEKTKNELIKHFKSVKR
                     IKSSNLDELTKIIGTNRASIVYKYFHPVLNA"
BASE COUNT          812 a          523 c          516 g          984 t
ORIGIN      
        1 cagatgtatg taaaaaaata tttccccgcc aaatcaaaag agcgcatggt tcagttggtt
       61 aagaacttac aaacagcttt gggtgaacgt attaacggtc ttgaatggat gggcgacagc
      121 accaaagcca aagcacagga aaaactcaaa acatttcata ttaaagtcgg ttatccagac
      181 aaatggcgcg attatacctc gctggatata caaaaggaca gttactgggc aaatataaag
      241 agagcgaata aattcgaaag tgattatatg tttgctaaaa taggtaaacc ggttgataaa
      301 gatgaatggt tgatgacacc tcaaactgta aatgcttact ataatccatc taccaacgaa
      361 atttgtttcc ccgcaggtat tcttcaatat ccgttcttcg atatgcaagc cgatgatgca
      421 ttcaattatg gatcaatcgg cgtggttatt ggtcacgaaa tgacccacgg atttgatgat
      481 cagggacgtc agtttgacaa ggatggcaac ctgaaagact ggtggaccaa ggaagatggc
      541 gaaaaattta atgcacgtgc caaagtgctt tcagactatt tcgataaaat tgaagtagca
      601 cccaatgtga aagcaaatgg taaattcacc cttggagaaa atatcgccga tcatggtgga
      661 ttacaggttt cttatcatgc cctgcaaaat gtattgaaaa cttctcctct tgaaacaaaa
      721 gatggattta ctcctgaaca acgtttcttc ctggcctatg ccaatgtttg ggcaggaaat
      781 attcgtcccg aagaaattct taaacgcacc aaatccgatc cgcactcatt gggaaaatgg
      841 cgtgttaatg gcgctttacc tcatattcaa gcctggtacg atgcatttaa tgtaaagcaa
      901 actaacaaaa tgtatgttgc acctgaacta agagcttcaa tctggtagag atataggact
      961 gataaacaaa tatccctgtc tgcttaattg caaacagggt tatttgttta tataaattaa
     1021 tcgtcgggaa tatcaatttt cttgtttttc ccgcgattgt acttgttttt aggctgggca
     1081 accttagtgt gtaaaacagg cttgccatgg gctttaattt catcctcacg gctttctttt
     1141 cttgcagctt ttaagacatc ctttatgggg ttgatttttt tcttttttgg tttcatgtcc
     1201 gtttaattta gtggagatat cactatcata cgaattccct tttcagtttc ttcgatgtca
     1261 acattcacgg tattgtcggg taggagagag gcaatgtttt ccgaccattc gataaagcag
     1321 taatttccgc tgtataaata ttcttctgct cccaggtcaa gagcttcctg aattttattg
     1381 atccggtaac agtcgaaatg ataaatgata ctgccatcgg acgcttcata ttcattcact
     1441 atagagaagg ttggactgtt cactgtttcc tttacaccca tctcttcaca aatcgctttg
     1501 atgaacgtag tttttccggc acccattttt ccgttgaaac aataaacggt ttccttattc
     1561 gatttcagat aagttaaaaa ttgtttagca gtaaatttta ttgtttgtat ggattttatt
     1621 tctaaaatag tcatcatagt cgttttattt tttagtgtct gattttttaa tgatttggtt
     1681 gtttttccgt tctgtatttg cgggaatttg ccgggaaaga ataaaatatt caattctcag
     1741 gcgttcaaaa ccgggtgaaa atatttgtaa acaattgatg ctctgttagt tccaattatt
     1801 tttgtaagtt catctaaatt agatgattta attcttttca cgctcttaaa atgtttaatt
     1861 agttcatttt ttgttttttc gccaataccg cgaatattat caagttcgga agctgtctga
     1921 tttttgcttc ttttgtcccg atgaaaagta atcgcaaatc gatgaacttc ttcctgaata
     1981 aatgcaagta atttgaaaag tgtgtcgttt ggtttcatcc cgatttcttt cggaggaaaa
     2041 ccgtagagca attcactggt tttatgctta tcattttttg caagtcccgc aataggaatt
     2101 ttaagattta attcatcttc caccacctgc cggattacct ccatttgacc gatacctccg
     2161 tcagcaatta tcaaatcggg taaaggcgcg ttttcattta ccattcgtat atagcgtctt
     2221 ttaacaactt ctttcatcga tgcgtaatcg tccggaccct gaacggtttt tattatatat
     2281 ttacgataat cctttttaga aggtttagct tttttgaaaa ccacgcaggc tgcaaccgca
     2341 tcggatcccg atatatttga attatcgaaa gcctcaattg ttattggtat tttttcaaga
     2401 tgtagttttt gctgaatttc cgttaataac tgaatgctct tttgatcagg atttaatttt
     2461 gctgtctgtt tgagtttttc aagtttatat tgttttacat tttgttcgga cagttcgagt
     2521 aattttttgc gatcaccacg ccggggaaca ataaacttta tatacgcaaa ttctatttcg
     2581 atagggaaag gtattattat ttccttagaa ttgctgttaa gtttttctct taattcaatg
     2641 attgcaatcg aaagtatatc ctcttttgtt tcgtctatcc tttttttata ttcaatagta
     2701 taaccctgaa taatcgcacc cttcgacacc cgtaaaatat tcacgtaagc agcgttttca
     2761 tcctcggcat aagcaaaaac gtctgtatta tttaccaccg aattcgcaat aatcgatttt
     2821 gatttataat tttca
//
LOCUS       DAOPYB010000303         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_49325_length_2834_cov_9.302627, whole genome shotgun sequence.
ACCESSION   DAOPYB010000303 DAOPYB010000000
VERSION     DAOPYB010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49325_length_2834_cov_9.302627"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..435)
                     /locus_tag="PKH58_13385"
     CDS             complement(13..435)
                     /locus_tag="PKH58_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90062.1"
                     /translation="MNTLPEKAISIALRVHAGQPDKAGAPYILYPLRLMLQMKTENER
                     LVAILHGAIEDSETRPEYLRLIGFPAEVLQSVDLLTHGESDSYEEYPAGIKPGTPACS
                     TKFAGLHDNLQLNRIKSPAAKDDQRTEKYRKAIAFLRL"
     gene            712..930
                     /locus_tag="PKH58_13390"
     CDS             712..930
                     /locus_tag="PKH58_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90063.1"
                     /translation="MFSLSALARSGLFISGSKECRERDRDTVPAVGLKLTRMIQGFKA
                     RDGSRLKSGEERSADSALRHLNIIVNLN"
     gene            1295..2425
                     /locus_tag="PKH58_13395"
     CDS             1295..2425
                     /locus_tag="PKH58_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90064.1"
                     /translation="MKMDTALNIIWDKRYQTDNNYFVQTICVNKYGDESFLVGADLTT
                     GPPDWYQKLVFLKTNNNGDSIAMTYFTQGDPYTARLKALTYIDNHFKIYTTGFGAFIP
                     YYYGGLAIIDFDTCFNIMGYSGIPHSISSYNYIENLNDSVYLLAGHTIIAGTGNSWDL
                     AIAKYNLDNEDLGFNHYNYSQQAREYPGWLKCLSVANGNSIYVGGWTNGDGYFICPYL
                     DQVIVLVNYDSALNMRWARFYGGNACYLSSIVQATTDGGCIIGGMYYDPSNPDNLQDA
                     LIIKVDSLGLVTDVPGTATISSHDVILYPNPFHDYFVIQSGPQIAGAWFRMYDVTGCL
                     IIERKINDTYLQQDMTGSPSGIYIWQVIKDGKLIENGNLIKN"
     gene            2468..>2834
                     /locus_tag="PKH58_13400"
     CDS             2468..>2834
                     /locus_tag="PKH58_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90065.1"
                     /translation="MKTKLLTAAVFTVFALLSNMVFSQLNGFPLLPSGDTEDYMKYIR
                     NAVNVDGENYFTGYLSLSAYKNYNTNVMYDPGIPLQKLQLRGGNILLYGTHLSPNINP
                     TSKNGAILFSDIISLQYPHG"
BASE COUNT          815 a          533 c          633 g          853 t
ORIGIN      
        1 tggatgacag agctataacc gaaggaaagc gatggctttc cggtactttt cggttcgctg
       61 atcatctttg gctgcgggtg atttgatgcg gttaagctgc aaattgtcgt gcaggccggc
      121 aaactttgtg gagcatgccg gcgtgccggg ctttatcccg gccggatatt cttcatagga
      181 atcagattcc ccgtgtgtga gcaggtcgac agattgtaac acctctgcgg ggaaaccgat
      241 cagtctgaga tattcaggac gggtttcact gtcttcgata gcgccgtgga gtatagccac
      301 aagtctttcg ttttcggttt tcatttgaag catgaggcga agcggataca aaatgtaagg
      361 cgctcctgct ttatcgggct gaccggcatg taccctgagg gcgatggaaa tagctttttc
      421 cggtaaggtg ttcattttaa gggtatattt tcccaaaatt acaggcagcg cttatttccg
      481 gacaagcgtc ggacgactct cccaaacaag ttgttgatgt tggcagcctg gatatcgccg
      541 gcgaaggaat agaaaggcag gcatagggct acgcgcgccg gcgaaggtga tcaatagtca
      601 tttttgacta atttaattgt tttctttgct ttaagcctga tttgctttaa ctttgtttta
      661 accggatttg taatgaattc agatttataa taaattttaa aaattgtgct catgttttca
      721 ctatcggctc ttgcccgttc cggtttattt atttcaggca gtaaggagtg ccgggagagg
      781 gacagggata cggttcctgc cgttggcctg aaattgacgc gaatgattca aggttttaaa
      841 gccagggacg gatcccggtt gaaatcaggt gaagaaagga gcgcggacag cgcattgcgg
      901 catttgaata ttattgtcaa tttgaactaa taaaagcgtt atgaaatttt tgttggtatt
      961 aattgtcatg ataatccctt ttgcagggat atcacaaacg acattcaggg tgcaattcgg
     1021 aacggcctgc aatgagatac cctcagccat tacacaaaaa ggtgattatt attatgttgc
     1081 ttatcaatgt aaaccagaac ctgtaacatc cctgtgtgat tcgtatctta taaaacttaa
     1141 cgcaaatgga gagaaagtca aagaagtgca tttcgatgca gcttccgaca gcgggtatta
     1201 ttgtattaac aaaatagttt tcttaaatga tacggagttc ctaatatttg gctgttgccg
     1261 ggaaaatagt tctgaatatg ggaaattttg gattatgaag atggatacag ccttaaacat
     1321 catttgggat aagcggtatc aaaccgataa taattatttt gtacaaacaa tttgtgtaaa
     1381 caaatatggt gatgaatcat tcttagttgg cgcagattta acaacaggac cacctgattg
     1441 gtatcaaaag cttgtgtttt taaaaaccaa taacaacggc gattcaattg caatgaccta
     1501 ttttactcag ggtgatcctt atactgcacg tttaaaagct ttaacataca tagacaatca
     1561 ttttaagata tataccacag ggtttggagc ttttattcct tattattatg gtggtttggc
     1621 aataattgat tttgacacgt gctttaacat tatgggctat agcggaatac cacatagtat
     1681 aagcagttac aattatattg aaaatctgaa tgatagcgtt tatcttcttg caggtcacac
     1741 aatcatagcc ggcaccggca acagttggga cctcgctatt gcaaaatata atttagataa
     1801 cgaagatctg ggttttaacc attacaatta ttcacaacag gcaagagaat atccgggctg
     1861 gcttaaatgc ctttcggtag caaatggcaa cagcatctat gttggcggat ggaccaacgg
     1921 ggatggttac tttatttgtc cctacctgga tcaggttatc gtactggtta attatgattc
     1981 ggcgctaaat atgcggtggg cgcgatttta cggaggcaat gcctgttatc tgtcaagcat
     2041 agttcaagcc acaaccgatg gcggctgcat tatagggggc atgtattatg acccgtcaaa
     2101 tccggataac ctgcaggatg ccttgattat caaagtagac agtctcggac tggtcacaga
     2161 tgtaccgggt acggcaacta tatcttcaca cgatgtaata ctatatccca atccgtttca
     2221 cgattacttc gtgattcaaa gcggccctca aatagcgggg gcatggttca ggatgtatga
     2281 tgttaccgga tgccttatca ttgaaagaaa aatcaatgat acctacttgc agcaggatat
     2341 gaccggctcg ccatcaggaa tttacatctg gcaggtaatt aaggatggca agttgattga
     2401 gaacggcaat ttgattaaaa attagcctac ataaaataat tcttattcat taacaaaatt
     2461 cagaaatatg aaaacgaaat tgcttacagc cgcagtcttt accgtatttg ctttattgtc
     2521 aaatatggtt ttcagccagc ttaacggatt tccgttgctt ccttccggag atacggaaga
     2581 ttatatgaaa tacatccgaa atgcagttaa tgtagatggc gagaactatt ttacaggtta
     2641 tctttcatta tctgcctata aaaattacaa taccaatgtc atgtatgatc cgggtattcc
     2701 tttgcaaaaa ttgcagttaa gagggggaaa tattctgctt tatggtactc atctatctcc
     2761 aaacataaat cccacttcta aaaacggtgc aattcttttt tctgacatta tttcacttca
     2821 gtatcctcac ggca
//
LOCUS       DAOPYB010000304         2810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_49994_length_2810_cov_12.193103, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000304 DAOPYB010000000
VERSION     DAOPYB010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49994_length_2810_cov_12.193103"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2549)
                     /gene="secDF"
                     /locus_tag="PKH58_13405"
     CDS             complement(<1..2549)
                     /gene="secDF"
                     /locus_tag="PKH58_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HNX90066.1"
                     /translation="MQNKGLVRILGIALALVCLFYLSFNLVTNIYENKAEERSLSAKE
                     AFQKSADFASLSTKEKSDRLNSVGSDAYYNYLDSLAKKKVYLGYTLKECREKGVNLGL
                     DLKGGMNVTLEISVPDIIRALSNYNTTPTFNQALTLASERQKTSGKQYLDLFVQAYKE
                     IDPQAKLATVFSTFDLKDRISLSSTNEDVIKVLKTEIDGAINNSFNVLRTRIDRFGVV
                     QPNIQKLENAGRILIELPGVKEPERVRKLLQGSANLEFWETYELSEIYSNLIEANKAI
                     AAVSAVGTTPVVADTTKTEGQAKKPEAVSPVNKIDSLKAALDTKKSATTDSVKMREEF
                     RKENPLFSVLQLYVDNSGQPGRGPIVGIASAADTARINAYFKMKQVKELMPRDLGLRW
                     TVKAVDEAETVYQLVAIKITSRDGRAPLTGAVITDARDEFSQTSANANVSMTMNAEGA
                     KIWARMTKENVGKSIAIALDGYIYSYPTVNGEITGGSSQITGNFTVEEAKDLANVLKS
                     GKMPAPARIVQEDVVGPSLGQDAVNNGLWSFVIAFLLVMIYMIVYYGLIPGIIADLAL
                     FANVFFLMGILASFGAVLTLPGIAGIVLTLGMAVDGNVLIYERVREEKEAGKTQKKAV
                     QEGLSHAMSAIIDANVTTLITGIILFIFGSGPILGFATTLVIGIITSFITSVFLTRLM
                     LETYVSRENAKDLAFTTKATSAFLQNTKVDFIGKRKTFYIVSGVVILICILGLTFRGL
                     SYGIDFTGGRNYIVQFEEKVNVEKVRSLLENTFEESSAQVISIGTNNDKVRISTKYKI
                     EENSETVDNEIDAKLYNGLKPLLKNASLDQFVKKNIVNSQKVGPTIAEDIKT"
BASE COUNT          820 a          588 c          513 g          889 t
ORIGIN      
        1 gtctttatat cttcagcaat agttggccct actttctgcg agttcactat atttttcttt
       61 acaaactggt cgagcgaagc atttttcaac aatggtttga ggccgttgta aagtttcgca
      121 tctatttcat tatctactgt ttcagaattt tcttcaattt tatatttcgt tgaaatacga
      181 actttgtcgt tattagttcc aatactgata acctgtgcgc ttgattcttc aaaagtattt
      241 tccagaagac ttcttacttt ttccacattt accttttctt caaactgaac gatatagtta
      301 cgacctccgg tgaaatcgat accataactt aaacctctaa atgtcagtcc cagaatacag
      361 ataagaatta caactcctga aacgatatag aatgttttac gtttaccgat aaaatcaacc
      421 tttgtatttt gcaggaaagc ggatgtagcc tttgttgtaa atgccaaatc tttcgcattt
      481 tcacggctaa cataagtttc caacattaaa cgggttaaga atacggatgt aataaaggaa
      541 gtaataatac caatcaccag agtagtggca aaaccaagta tcggacctga accaaagatg
      601 aacagaataa tacctgtaat aagggttgtt acgttagcat cgataatggc agacattgca
      661 tgactaagtc cttcctgaac cgctttcttc tgcgtctttc cggcttcttt ttcttcacgg
      721 acacgttcat agataagtac gttaccgtca accgccatac caagcgtcag cacgataccc
      781 gcgatacccg gtaaggtaag tacagcacca aaggacgcta aaatacccat caggaagaat
      841 acgttagcaa ataatgccaa gtcggcaatg atacccggaa ttaaaccgta atatacaatc
      901 atataaatca ttaccaacag gaacgcgata acgaacgacc acaaaccgtt gtttacggca
      961 tcctgaccaa gtgaaggacc aacaacatct tcctgtacta tacgtgccgg agcaggcatt
     1021 ttacctgatt tcaacacgtt tgccaagtcc ttggcttctt ctacagtaaa gttacctgta
     1081 atctgtgaac taccaccggt aatttcaccg tttacagtcg gatatgagta aatgtaacca
     1141 tccagcgcga tagcaatgga tttgccaaca ttttccttgg tcattcttgc ccatattttc
     1201 gcgccttcgg cattcatcgt catacttaca ttagcgttag ctgaagtctg actgaattca
     1261 tcgcgggcat cggtaatcac agcaccggta agcggagcac gtccgtcgcg gcttgtaatt
     1321 tttatggcta ccagctgata aacagtttca gcttcatcaa cagcttttac agtccagcgt
     1381 aaaccaaggt cacgtggcat cagctctttc acctgtttca ttttgaaata tgcattgata
     1441 cgtgcagtat cggcagcact tgcaatacct acaataggac cacgaccagg ttgacccgag
     1501 ttgtcaacat aaagttgaag cactgagaac aatggatttt ctttgcggaa ctcttcacgc
     1561 atttttacag aatctgtagt tgctgatttt ttggtatcaa gagcagcttt caggctgtcg
     1621 attttattaa caggacttac agcttctggt ttctttgcct gaccttcagt ttttgttgta
     1681 tctgcaacaa caggagttgt tccaacagca ctaacagcgg caatagcctt gtttgcttct
     1741 atcaggtttg aatatatttc tgacagttca taagtttccc agaattcgag gttggcagaa
     1801 ccctgaagaa gtttacgtac acgttcaggt tcttttacac ccggaagttc aatgaggata
     1861 cgtcctgcat tttcgagctt ctggatattt ggctgtacca caccgaaacg gtcgatacgg
     1921 gtacgaagta cgttgaatga attattgata gctccgtcaa tttcggtttt taacaccttg
     1981 attacgtctt cattggttga gcttagtgag atacggtctt tcaggtcaaa agtactgaat
     2041 acagtagcca gttttgcctg aggatctatt tccttgtaag cctgaacaaa aaggtcgagg
     2101 tattgttttc cactagtttt ctgacgttcg gaagccagag tcagtgcctg gttgaatgta
     2161 ggtgtagtgt tgtaatttga aagtgcgcga atgatgtccg gaacagagat ttccagcgtt
     2221 acgttcatac cacctttcaa gtcaagacct aaattcaccc ctttttcacg acattctttc
     2281 agtgtatatc ccagatatac ttttttcttt gccagtgagt ccaaataatt atagtaggca
     2341 tcactaccaa cagagttcaa ccggtcactt ttttctttag ttgaaagtga agcgaaatca
     2401 gctgatttct gaaaagcctc ttttgctgat aaagatcttt cttctgcttt gttctcgtaa
     2461 atattggtca ccagattaaa tgacaaatag aataaacata cgagtgcaag tgcaattcca
     2521 agaattctta caagtccttt gttttgcatg gaaaatttat tttaattaag tttattatta
     2581 cagttttttt aaacgagctg caaatataca atttttttta ataatgacac aaaaaatgta
     2641 caattccatg actcaaactt tgttaatgag agaattctac atatcttact ttccaaatat
     2701 tttgagtaaa tccagttgtg ctgaaattac ttttcctgtc cccggacaaa ataaatcaaa
     2761 tattatttgg tgataaggaa aaattatagt acttttgtac tcacaaaaca
//
LOCUS       DAOPYB010000305         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_50356_length_2798_cov_6.892089, whole genome shotgun sequence.
ACCESSION   DAOPYB010000305 DAOPYB010000000
VERSION     DAOPYB010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50356_length_2798_cov_6.892089"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2332)
                     /locus_tag="PKH58_13410"
     CDS             complement(<1..2332)
                     /locus_tag="PKH58_13410"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX90067.1"
                     /translation="MRILKISGLMLLLFFLMHGKGYNQTIKPTQVSGLTLWLRADSAL
                     VLNGANVVQWSDCSGNNNNAMQVDNSMQPAFIDSVSSINNKPAIRFDGINDFLAFNEI
                     STIRTCFFVCKHATGYGDYNTTLGHSSLYHFVGGTGNHLFSSDYASQDILQGSIFENS
                     IGSSPSTITKPTKYSIVSLITTGNVAASNIGSDRGISNHFWNGDFVEIIIYSGALNNS
                     DRITVEKYLDRKYAGPPVNLGPDINIPYGFCNTTLDAGSRFTHFLWSTGDTTQIISVG
                     ASGTYSVTATDYFGYLTSDTINVIFPHIALYDTVFCTGSNVVLSTGLAGNYGYLWSNG
                     LTTPTISVNSPGAFSVTVTDSLSCYKESDTVYVGEDDFYSTVSLGSDTTLCAGELIGL
                     VSSGPLPSGMTYHWSTSDTTAEIPISTSGDYSVTVTNVNGCIAIDTVFVTIAGIVPNV
                     DFSFNTACSGLASQFNNTSTPGGTTWLWNFGDGQTSALENPSHVYATGGDYNVSLLCS
                     DGNCSNVISKTIHMPYSPSVAFAVSQTCINIPYSFIDQSFVSEGSIVIWDWNFGDGSP
                     HSAVQNPQHIYALPGNYDVSLTVQTDSGCTDIATHQITVVGSAPAPSVFTLLHPVDNF
                     VTTGHIINFAWNITPGAVTYTLEYSVDSLFTNNVIAIPGLQGPTCQQNIGLPQTYYWR
                     VIAYNLCGDSIISPVFSFTFLSLNSIPGLQIWLRADSNVVLNGNKVSHWNDCSGNGNH
                     ALQPVAGSQPLIIDSVLNGLPVVNFNGSGYLSSVFTS"
BASE COUNT          918 a          533 c          538 g          809 t
ORIGIN      
        1 aggaggtaaa aacagaactt aaataaccag acccgttaaa attaacaaca ggtagtccat
       61 tgagtacgct gtcaattata agaggctggg aacctgcaac aggctgcaat gcatgattgc
      121 cgttgccact gcaatcattc caatgactga ctttattccc atttaatacc acattactgt
      181 cggcacgcaa ccatatttgt aagccgggta tggaatttaa tgaaagaaag gtgaaagaaa
      241 agacaggcga aataattgaa tcaccgcata agttataagc aataacccgc caatagtatg
      301 tctggggtaa accaatgttt tgctgacaag tgggcccctg taagccggga atagcaatta
      361 cattgtttgt aaataatgaa tcaactgaat attccaaagt ataggtaact gctccgggcg
      421 taatattcca tgcaaaattg ataatatgac cggtggtaac aaaattatcc acaggatgaa
      481 gaagtgtaaa taccgatggc gcaggggcgg aaccgaccac cgtgatctgg tgcgtggcga
      541 tgtctgtgca tccgctgtcg gtctggacag tcagggaaac atcgtagttc cccggcagcg
      601 cataaatgtg ctgggggttc tgaaccgcgg aatgggggct tccgtcgcca aaattccagt
      661 cccatattac aatactgcct tctgatacaa aactttgatc aataaaacta taggggatat
      721 ttatacaggt ttgagatact gcaaatgcta ccgatggtga atagggcata tgaatagttt
      781 ttgatattac attgctgcag tttccatcgg aacataacaa actcacatta taatccccac
      841 cggtagcata tacatgtgaa ggattttcta aagcagaagt ttgtccatcg ccaaaattcc
      901 acaaccaggt agtacccccg ggtgttgaag tgttattaaa ctggctggct aatccggagc
      961 aagcagtatt aaaactaaag tcaacattag gtactattcc ggcaatggta acaaaaaccg
     1021 tatctattgc aatgcaacca ttaacattag ttactgtaac agaataatcg ccagaagtgc
     1081 ttataggtat ttcagcagtg gtgtcacttg tggaccaatg gtaggtcatc cctgatggta
     1141 agggaccgga agacaccaag ccgattaatt cccctgcgca taatgttgta tcactgccaa
     1201 ggcttaccgt actataaaaa tcgtcctctc cgacatatac agtatctgat tctttatagc
     1261 atgacaaact atcagtaacg gtaacggaaa aagcacctgg cgaattaaca gaaattgttg
     1321 gtgtagtcaa cccatttgac caaagatatc catagttccc ggctaacccg gtcgaaagaa
     1381 caacattact cccagtacaa aatactgtgt catataaggc tatgtgggga aaaattacat
     1441 taatggtatc ggaagttaaa tacccaaaat aatcagttgc tgttaccgaa tatgttccgg
     1501 aagccccgac actgattatt tgtgtcgtat cacctgtaga ccaaagaaaa tgtgtaaatc
     1561 tgcttccggc atctaatgtg gtattacaaa agccataagg aatatttata tcgggaccaa
     1621 ggttaacggg aggtccggca tattttcggt caaggtattt ctctacagta attctatccg
     1681 aattatttaa agccccacta tatataatga tttctacaaa atccccattc caaaaatgat
     1741 tggatattcc cctgtcgctt ccaatattgc ttgcagcaac attccctgtc gtaataagag
     1801 atacaatgct atattttgta ggtttcgtta ttgttgatgg tgatgaacct atgctgtttt
     1861 caaaaatact cccttgtaaa atatcttgag aggcataatc tgaagaaaat agatggttcc
     1921 ctgtacctcc tacaaagtga tacaaagatg aatgtcctaa tgtagtattg taatcaccgt
     1981 atcctgtggc gtgtttacat acaaaaaaac atgtgcgtat agttgaaatt tcattaaaag
     2041 ccaaaaaatc attgatgcca tcaaaccgta tagctggttt gttgttaata cttgaaaccg
     2101 aatcaataaa ggctggttgc atggaattat caacttgcat agcattatta ttatttccgc
     2161 tgcagtcgct ccattgcaca acgtttgcgc catttaatac aagggccgaa tcagcccgca
     2221 gccataaagt caagccagag acttgggttg gtttgattgt ctggttatag ccctttccgt
     2281 gcattaaaaa aaacagcagg agcatcaagc cggaaatttt caaaatacgc atataggaga
     2341 aattttatac aaatataatg gcttggcacg gaaaatcaaa ataaaatttt ataatggatt
     2401 tttgttatga ctgaaaccaa taaagcacgc caaattcaaa ttttcaaact catctggcag
     2461 atcaaaacca aaacctctta atgtttaatt tgtaactgaa cattttttta ctaaagttcc
     2521 gcctcggcgg gccatgctga caattttaaa aaataaaata ttcaagtagt ttggagtttt
     2581 agtcctaatc ggcattgata tctggtaact gagttcttaa aattattatt tttcattatt
     2641 gttttattat ttgataattt tgtatttcaa tttgataaat gaataaaatg ctcagaagta
     2701 aaaaaagcat tcaaagcctt acaataattt ttattgtgtt ttttcttagt acatgacaaa
     2761 actcgtaact tattgataat gttgataata atgttaat
//
LOCUS       DAOPYB010000306         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_50379_length_2797_cov_11.741430, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000306 DAOPYB010000000
VERSION     DAOPYB010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50379_length_2797_cov_11.741430"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..2741)
                     /locus_tag="PKH58_13415"
     CDS             complement(402..2741)
                     /locus_tag="PKH58_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNX90068.1"
                     /translation="MNRTKAFILLFLFVCSVVSFSQATIKYKDPTLSTEERTQDLLKR
                     MTLEEKVGQLLCPMGWEMYERKGNNISTSALFREQTNTIKAGMYWAVFRADPWTKKTL
                     DNGLTPELAAKAANALQEYTIKNTRLGIPLFFAEEAPHGHMAIGTTVFPTGIGLASTW
                     SEETMYKVGKIIALEIRLQGAHIGYGPVIDLVRDPRWSRLEETFGEDPVLAGILGSAL
                     INGMGGGNLKENSSVISTPKHFIAYGIPESGQNGASTSIGMRELFTDFLPPFHQLVKS
                     GALSIMTSYNSIDGIPCTSDKMLLKGVLKDKWNFRGFVVSDLFAIDVIHNGHNVAETL
                     EDAAIKAITAGTDVDLGANSYQHLVNAVKSGKVDESVVDSAVVRVLRLKFDMGLFENP
                     YVDPKMAKLKVRSEENVRIALQAAKESIILLKNSNNILPLNPELKVAVIGPNADNIYN
                     QLGDYTAPQDPKQIVTVLKGIQNIVNEKNIRYVKGCSIRDTVNTQLNEAKEAALQSDV
                     VIMVMGGSSARDFKTSYKETGAAETNANIISDMEAGEGFDRTTLSLMGKQLDLLKAVH
                     STGKPMIVIYIAGRPLDMNWAEANANAILMSWYPGQEGGNAIAGVLFGNYSPAGRLPV
                     SVPLNVGQVPVYYNKKRPKTHDYVESPSTPLYPFGYGLSYTNFQYSNLLVKRIGQEEF
                     DVTFLIKNTGKYDSDEVVQLYLNDEYSSVVRPEKELRKFSRIFIRANDQKEIHFTLKK
                     ADFELISEDMKRVVEKGAFNILIGASSTDIRLRERIIIE"
BASE COUNT          806 a          594 c          481 g          916 t
ORIGIN      
        1 tcagcggaat agcaactttt cttacgttca gacctctatc ccttcccgaa gtatcggaac
       61 acctttctga cctcacccgc ccttcgggca ccctctccaa aggagaaata tgttgaatat
      121 ttcaaaatat cagcctcatg tcatttttac aaaaacaggg gataagagac tttccagtcg
      181 ctttattaat aattaaaacc gcaatgtcct ggttacgcca accgaagcta ccccaaatac
      241 tttcagaata taatgagtaa taagatttat tcaatcaatt gaaaatcaaa caggagaaaa
      301 cttgtttttc ccaaacctgc aaactcaaaa aaatgaagta gctgaagatt ataattttat
      361 atccgaatag ctgcaaaatg ggttaatatt caggaaagag attattctat tatgattctt
      421 tctctcaaac gaatatctgt tgatgatgct ccaattaaaa tgttaaatgc tcctttctct
      481 actacacgtt tcatgtcttc agaaattagt tcaaaatcag ccttctttag tgtgaaatga
      541 atttctttct gatcattagc tctgatgaaa atgcggctaa acttacggag ttctttttcg
      601 ggacgcacaa cagatgaata ttcatcgttc agataaagtt gtaccacttc atcgctatcg
      661 tattttcctg tatttttaat aaggaatgta acatcaaatt cttcctgacc tattcgtttt
      721 actaataaat tactgtattg aaaattagta taactcaacc catatccaaa cggatagagt
      781 ggggtggacg gggattctac ataatcgtga gttttcggac gttttttatt gtaataaaca
      841 ggaacttgtc caacattcag aggaacagaa acaggtaatc ttcctgccgg actatagtta
      901 ccaaacaaaa caccggcaat ggcatttcca ccttcctgac cgggatacca ggacattaaa
      961 atagcattcg cattggcttc tgcccaattc atatccagag ggcgacctgc tatataaatt
     1021 acaatcatgg gtttaccggt agaatgaaca gctttcaata aatcaagctg tttccccatc
     1081 agtgaaagag tagtacggtc aaatccttcc ccggcttcca tgtcactaat gatgttagca
     1141 ttggtttcag ctgcaccagt ttctttgtat gatgttttga aatcccgggc acttgaccca
     1201 cccattacca ttattactac atccgattgt aatgctgctt ctttagcttc atttagttga
     1261 gtattcacag tatcccgaat tgaacaccct tttacatacc ggatattttt ctcattaaca
     1321 atattttgta tcccttttag tactgttact atctgtttcg ggtcttgtgg tgcagtataa
     1381 tcacctaact gattgtatat gttatcggca tttggtccta tcacagctac ctttaattcg
     1441 gggttgagtg gtagaatatt gttactgttt ttcaataaaa taatagactc ttttgcagcc
     1501 tgtaaagcta ttcgaacatt ttcttcactt ctgactttca gtttggccat ttttgggtct
     1561 acataaggat tttcaaatag tcccatatca aattttaatc gtaaaactct gacaactgct
     1621 gaatcaacta cagattcgtc aaccttacct gatttaacag cattaaccaa atgttggtaa
     1681 gaattggcac ccagatctac gtctgttcct gcagttatgg ctttaattgc tgcatcttcg
     1741 agtgtttctg caacattatg cccgttgtga atgacatcga ttgcaaataa atcggacact
     1801 acaaatcctc tgaaattcca tttatctttt aatacccctt tcaataacat tttatcggat
     1861 gtacatggga taccgtcaat agaattataa gatgtcataa ttgaaagcgc tccggactta
     1921 acaagctgat gaaagggtgg taaaaagtca gtaaataatt cacgcatccc tatggatgtg
     1981 gaagcgccat tttgtccgct ttcgggaatt ccgtaggcga taaaatgttt tggcgttgat
     2041 attaccgagc tattttcttt tagattgcct cctcccatac catttatcaa agcagaacct
     2101 aatatgcctg ccagcaccgg atcttcacca aatgtttcct ctaaccttga ccaacgggga
     2161 tctcgtacca aatctattac cggaccataa ccaatatgag cgccttgaag tcttatctct
     2221 aaagcaatga tttttcctac cttatacatc gtttcttccg accatgttga agcaagacca
     2281 ataccagtag gaaaaacggt tgtccctatt gccatgtgtc catgaggagc ttcttctgca
     2341 aagaataaag gtatgcctag ccgtgtgttt tttatagtgt attcctgaag tgcatttgct
     2401 gcctttgcag ccaattcagg tgttaaaccg ttatcgagcg ttttttttgt ccagggatca
     2461 gccctgaaca cagcccaata catacctgct tttatagtgt ttgtctgttc tctgaataat
     2521 gcagatgtgg agatgttatt tccttttcgc tcatacattt cccaacccat cggacaaagc
     2581 aactgtccga ccttttcttc cagcgtcatt cttttaagca ggtcttgtgt acgctcttca
     2641 gtgctaagtg ttggatcttt atattttatt gttgcttgtg aaaaggacac gacgctgcaa
     2701 acaaacaaaa atagcagaat aaatgcttta gttcgattca taattgggat ttattgttgt
     2761 tcggtaaaaa tatttagttc cttcgtcccg agaattc
//
LOCUS       DAOPYB010000307         2794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_50454_length_2794_cov_8.049653, whole genome shotgun sequence.
ACCESSION   DAOPYB010000307 DAOPYB010000000
VERSION     DAOPYB010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50454_length_2794_cov_8.049653"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PKH58_13420"
     CDS             <1..1415
                     /locus_tag="PKH58_13420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX90069.1"
                     /translation="LALPELLEYLQLTDIYLFTSNDPNQAVSGTFVYAMSCGCPIIST
                     PIPHAKEVLTSDTGIIIDFQNSEQLSEGVLRLFNDEPLRKSISTNTLQKIVFTAWENS
                     AVAHAKLLAEQMGRKLQISYILPEIKLDHLTKMTTDFGIVQFCKINQPDPTTGYTLDD
                     NARALIAVCMHYELTKDASDLKLIKIYLDFIKFCQQSGGNFLNYVDIDKNFTQQNEEV
                     NLDDSNGRTLWALGFLISKKSILPKEFISSATTMLQLSLPHSKTITSTRALAFIIKGL
                     YFYNKEIISAENKLLTTQLADRLVAMYRHESTKNWEWFESYLTYANSILSEAMLCAYL
                     VTRKASYRLIAVESFNFLMSNIFNENGIEVISNKHKFNKGGIAPQFGEQPIDVAYTIL
                     TLSKFHEEFNNPDYLNKMRTAFNWFLGENRLNQIIYNPCTGGCYDGLEEHNINLNQGA
                     ESTVSYLMARMTVERYKDEQ"
     gene            complement(1581..>2794)
                     /locus_tag="PKH58_13425"
     CDS             complement(1581..>2794)
                     /locus_tag="PKH58_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90070.1"
                     /translation="NYGRSESDVPPRQGTWDYKGSRIWINDKEILPPTWGSQHTSRDN
                     ETPLTNENSAVRTPISVNLVQGWNKVFMKLPVGAFSISQVRLVKWMFSCVFVTPDGKD
                     AVEGLIYSPDQNMNPSADALITAIDDAEAFKNSTEKGLQPGEYPAAAFDSFDVAITAA
                     RTAKLQSTTDEQFSAAVNELVKGMQQYKNEINKPLASTANETVWYNLYTPYRDNRYLA
                     YKGDNVNLLGEAYVVGADRQLWKIIKGTDGSYAIVSKVANSSISPASANNSALKTQAG
                     IPTSGGWTFTPTYTNKTFIVTSGSVQFNQTNSGLGYFIYNWGNGTNLTDTGCRYLITE
                     ASRMSTDVPANLQSDNKVWSSNRKIMIDGDYTQVSLYSPEGRKLNLSGPQEPGLKIIN
                     IDGVAYKVMVK"
BASE COUNT          802 a          607 c          552 g          833 t
ORIGIN      
        1 atctggcgct acccgaattg ctcgaatatc ttcaacttac cgatatttac ctgtttacca
       61 gcaatgatcc taatcaggct gtcagcggca cttttgtata tgccatgagc tgtggttgcc
      121 ccattatttc cacacctata cctcacgcca aagaagtgct tacatcagac accggcatca
      181 tcattgattt tcagaattcg gaacaattat ccgaaggagt tttgcgtctt ttcaatgacg
      241 agcccttgcg caaaagcatt agtacaaata ccttacagaa aatagtattt actgcctggg
      301 aaaattcagc ggtggcacat gccaaattat tagccgaaca aatgggacgg aaattgcaaa
      361 tttcatacat acttcccgaa atcaaactcg accatttgac caaaatgacg accgattttg
      421 gtattgttca gttttgtaaa attaatcagc ctgatcctac tacgggttac acgctcgacg
      481 ataatgcccg ggcgctgatt gccgtctgca tgcattacga actgacgaaa gacgcaagtg
      541 atttgaaact gatcaaaatc tacctcgatt tcataaaatt ctgtcagcag tctggaggaa
      601 atttccttaa ttacgtagat attgataaga attttaccca acaaaatgaa gaagtcaatc
      661 ttgatgattc aaatggcaga actttatggg cgctgggatt tctgatttct aaaaaatcaa
      721 tactgcccaa agaatttatt tcttccgcta caacaatgct tcaactctcg cttcctcata
      781 gcaaaacaat cacttcaacc cgtgcgttgg cattcattat caaaggattg tatttttata
      841 ataaagaaat tatttctgca gaaaataaat tgctgaccac tcaacttgcc gatcgcctgg
      901 tagctatgta tcgccacgaa tcgacaaaaa actgggaatg gttcgagagt tacctgactt
      961 atgccaacag catattgtcg gaagccatgc tgtgtgctta tttagtgacc cgaaaagcca
     1021 gttacaggct cattgcagtg gagtcattca atttcttaat gtccaatatt tttaatgaaa
     1081 acggtattga agttatttcg aacaaacaca aattcaataa aggcggaatt gcaccacagt
     1141 ttggcgagca acccattgat gtggcttata ccatactgac actgagcaaa tttcacgagg
     1201 agtttaataa tccggactat ctgaacaaaa tgagaacagc ttttaattgg tttctgggcg
     1261 aaaacaggct caatcagatt atttacaatc catgtacagg tggttgttac gatggacttg
     1321 aagaacacaa catcaatctt aatcagggtg cggaatccac tgtaagttat ttgatggcac
     1381 gcatgactgt tgaaagatat aaagatgaac aataaaaaaa gctagctaat taaataaaaa
     1441 ctgtaataat ccccgcacaa tgtttcggtt cgctgcacct aaataaaagc cacagatatg
     1501 tgaattttaa ggtacagcgc accaaaacat ttttagttga gattttcagc agacaccgga
     1561 taatctccag ttaatatcta ctatttcacc attactttgt aagcaacgcc atcaatgttg
     1621 attattttta gccccggttc ctgcggtccg gacagattta atttacggcc ttcgggtgag
     1681 tacagactga cttgtgtata atccccatca atcattattt tccggttact gctccacact
     1741 ttgttatcgg attgtaaatt ggcaggaaca tccgtactca tacggctggc ttccgtaatc
     1801 aaataccggc aaccggtatc tgtgaggttt gttccattac cccaattgta aataaaatat
     1861 cccagtcctg agttggtttg gttaaactga acagaccctg atgttacaat aaaagttttg
     1921 tttgtatagg ttggtgtgaa agtccatcca cctgaagtcg gaatacctgc ctgagttttc
     1981 agagcgctgt tatttgcaga agcagggctg atggaactgt tagctacttt acttactatt
     2041 gcgtaacttc catcggtacc ttttataatc ttccaaagct gacggtcggc accaactaca
     2101 taagcttcac ctaaaaggtt aacgttatca cctttatatg ccagataccg gttatcacga
     2161 taaggtgtat agaggttgta ccataccgtt tcgttggctg tactggctaa tggtttgttt
     2221 atttcatttt tgtattgctg cattccctta accaactcat tgactgcagc gctgaattgc
     2281 tcatcggttg tactctgcaa ctttgctgtt cttgctgcgg tgatggctac atcgaaagag
     2341 tcgaatgcag ccgcgggata ctcccccggc tgaagccctt tctctgttga gtttttgaat
     2401 gcttcggcat catcaatggc tgtaatcagg gcatcggccg aaggattcat attctggtca
     2461 ggtgaataga tcaacccttc tactgcatct ttgccatcgg gtgttacaaa tacacaggag
     2521 aacatccatt taaccaggcg aacctgtgaa atgctgaaag caccaaccgg cagtttcatg
     2581 aaaactttgt tccatccctg taccagattt actgaaattg gcgtacgaac cgctgagttt
     2641 tcgtttgtga gcggtgtttc attatcgcgg gatgtatgtt ggcttcccca ggttggtggc
     2701 aatatttctt tgtcattaat ccagatgcgg cttcctttat agtcccaggt accctgtcga
     2761 ggtggcacat ccgattcaga acgcccataa ttct
//
LOCUS       DAOPYB010000308         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_50513_length_2792_cov_7.509682, whole genome shotgun sequence.
ACCESSION   DAOPYB010000308 DAOPYB010000000
VERSION     DAOPYB010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50513_length_2792_cov_7.509682"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PKH58_13430"
     CDS             <1..1063
                     /locus_tag="PKH58_13430"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90071.1"
                     /translation="TLFFHLDILNKSASEYQNTFLTIFTDFDLGNNLDDYIGTDVTHG
                     MVYAYNGTEVDGEGESYAYGAHPPAIGMCILGGPYMDADGTDNPDTGCGYNTERFNFG
                     DGIADNERFGLCGSNSQFNYPPSWYFEDDFAINYYSYMCNLFRDGVHLNFGGSGRPDG
                     GSVGPDCMYIYPGNSDTLCNFGTGGVMPNGGYNQNGYYWTEGTVDNAPGDRRMIANIG
                     PFTFQVGQSLPLDYCFIFARDYQGTNLTSVDLLQQYSEEITSQAGNLTILPGTYYSVK
                     ENDKSGKLKVIPNPVTDNVRIFYTEASTIPFLVSDINGQVRKSGNLANGTADINLNDL
                     KPGIYIIRSGSCSAKIVKL"
     gene            1188..2312
                     /locus_tag="PKH58_13435"
     CDS             1188..2312
                     /locus_tag="PKH58_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX90072.1"
                     /translation="MIKREVEAAILKRMDKEKIILLFGARQVGKTTLLKQLTQSGQYK
                     TTWLNADDAVVQALFDNFSEASFTAYIGNPEIVVIDEAQQIMNIGKCLKILYDSGLTF
                     QLIASGSSAFELRNKTSEPLTGRKWEYHLYPFSFKEMAEETNPITEVQQLPLRLLFGM
                     YPEILLHKGEERDRLQWLMDSYLYKDVIMWQGLKKPEKIIQLLKALAMQTGSEVSYNE
                     LSGLIKLDRETVEKYINILEEAYIIFRLPSYSTNKRKELSKSRKIYFFDNGIRNSLLG
                     DFRPIEARQDTGALWENYIISELWKSNQYRMQYSSFYFWRTSDQQEIDLIIEKDGLIN
                     AYEIKWNPVKKARLNKTFINYYPHHTFNVINRDNYAMFLL"
     gene            2584..>2792
                     /locus_tag="PKH58_13440"
     CDS             2584..>2792
                     /locus_tag="PKH58_13440"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90073.1"
                     /translation="MRTTLFVNVLLPLPVAGYFTYRVPYELNESVAVGKRVVVQFGQK
                     KIYTALIRETTESPPPVQPKYILSVL"
BASE COUNT          841 a          562 c          583 g          806 t
ORIGIN      
        1 tacactgttt ttccatctcg acattctgaa taaatcggct tctgaatatc aaaacacctt
       61 tcttacaata tttacggatt ttgatcttgg aaataatctc gatgattata taggtaccga
      121 cgttacgcat ggaatggttt atgcctacaa cgggacagag gttgacggcg agggggaatc
      181 atacgcttat ggtgcccatc ctcccgccat aggtatgtgt attttaggag ggccttatat
      241 ggatgcagac gggactgaca atcctgatac aggatgcgga tacaataccg aacgatttaa
      301 tttcggtgat ggaatagctg ataatgaacg gtttgggtta tgcggttcta attctcaatt
      361 taattatccc ccttcatggt atttcgaaga tgattttgcc atcaactatt atagctatat
      421 gtgtaattta ttcagagacg gggtacatct gaacttcgga ggatcgggcc gccccgatgg
      481 aggcagtgtt ggacccgact gcatgtatat ttatcccggc aactccgata cgctctgtaa
      541 ttttggcacc ggtggcgtga tgccaaacgg gggttacaat cagaacggat attactggac
      601 tgagggaact gtggataacg ctcctggtga ccgcagaatg attgcgaata tcggaccgtt
      661 tacttttcag gttggacaat ctttgcctct tgactattgt ttcatctttg cccgtgatta
      721 ccagggaacc aaccttacat ccgttgattt gctgcaacag tacagtgaag aaattacctc
      781 acaggccggg aatttgacta tcctgcccgg gacctactat tctgttaaag aaaatgataa
      841 atccggtaaa ctgaaagtta ttcccaaccc ggtaactgac aacgtaagaa ttttttatac
      901 cgaagcatca accattccat tcctggtttc cgacatcaat ggtcaggtga ggaaaagcgg
      961 caaccttgct aacggaactg ccgatattaa tctgaatgac ttaaaaccgg gcatttatat
     1021 catccggtca ggaagctgca gcgccaaaat agttaagctg taaagctctg ttcttcaatt
     1081 agccctgtca gtattttgaa ttctgacagg gtaatatttg attttttgta taattctcgg
     1141 ttgtaaccgc atatttatcg gtatttttgt ggaattaaac cgaaaatatg attaagagag
     1201 aagtcgaggc tgctatattg aaacgcatgg acaaagaaaa aataattctt ttgttcggag
     1261 ccagacaagt tggaaaaacc acactcctga aacagttgac ccaatcaggc caatacaaaa
     1321 ccacctggct taatgcggac gatgctgtag tacaggcatt gtttgacaat ttttctgaag
     1381 cttcattcac agcctatatc ggcaatccgg aaattgtagt aatagatgaa gcccagcaaa
     1441 ttatgaatat tggaaaatgt ctcaaaatcc tttatgattc cggtctgaca tttcaactaa
     1501 ttgcttcagg atcatccgct tttgaattac gcaataaaac aagcgaacca cttactggaa
     1561 gaaaatggga atatcatctt tacccattca gtttcaagga aatggcagaa gaaaccaatc
     1621 ccattaccga agtccagcaa cttcctttac gacttttatt tggcatgtat cccgaaatat
     1681 tacttcataa aggcgaggag cgtgacaggc tgcaatggct gatggacagt tacctttaca
     1741 aggacgtcat tatgtggcaa ggattgaaaa aaccggaaaa aatcattcag cttttaaaag
     1801 cattggcaat gcaaactggt agcgaagtaa gctataatga actatcagga ttaataaaac
     1861 tggatcgtga aaccgttgag aaatacatta atatccttga agaggcttat atcatattcc
     1921 gattgccgag ttatagcaca aacaaaagga aagaactgtc gaaaagccga aaaatttatt
     1981 tttttgacaa tggcatacgg aacagccttc ttggtgactt cagacccatt gaagccaggc
     2041 aggataccgg tgctttgtgg gaaaattata ttatcagcga attatggaaa agcaatcaat
     2101 atcgtatgca atacagttca ttttatttct ggcgcacatc agaccagcag gaaattgacc
     2161 tgattatcga aaaagacgga ctgattaatg cctatgaaat aaaatggaac ccggtaaaaa
     2221 aagcaaggtt aaacaaaaca ttcatcaatt attatcctca tcataccttc aatgtgatta
     2281 accgggataa ctatgcaatg tttctgcttt gatttccttt gattttattc atagttttga
     2341 aaaaggtgac aagtgggagg agagaggaga atgtaaaata ttcattcatc cctggcaagg
     2401 ttttatttga tcatttttcg tacaattttc ggttgcatcc gcaattttac cggttttttt
     2461 gcggattata atcggttctt tttaaactat ttcaacgagt aacctttcca tattaaccaa
     2521 tcaaccattt accaaccatc taaaatccct accttcgtca tgtgctgcta aaaagcaacc
     2581 tccatgcgta ccaccctgtt tgttaatgtt ttactgccgc tgccggtagc cgggtatttc
     2641 acctaccgcg ttccgtatga gctgaacgaa tcggtggctg tgggcaagag ggtagtggtg
     2701 cagttcgggc agaagaagat ttatacggcg cttatccgcg aaactactga gtcgccgcct
     2761 cccgtacagc ccaagtatat tctatcggtg ct
//
LOCUS       DAOPYB010000309         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_50572_length_2790_cov_11.861426, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000309 DAOPYB010000000
VERSION     DAOPYB010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50572_length_2790_cov_11.861426"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..825)
                     /locus_tag="PKH58_13445"
     CDS             complement(100..825)
                     /locus_tag="PKH58_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX90074.1"
                     /translation="MKEANFPYAIGYGLTESAPLLAGAGTKQTKLGSTGFAVQGVELK
                     INNPDKNGLGEIWAKGPNVMLGYYKNPEATSEVLTPDGWFRTGDYGYLDSHKRLYIKG
                     RMKNTIIGASGENIYPEDIESVINNNQYVLESLVFEEDGDLVAKILLDLEELEKNIEH
                     IKSVIEEKKVKGLHTIHGLDDKYKTWSQHILKKEINDKLNRVSQIKRVELIDKPLEKT
                     ATQKIKRFLYTKHPAGEHKENKD"
     gene            complement(834..1811)
                     /locus_tag="PKH58_13450"
     CDS             complement(834..1811)
                     /locus_tag="PKH58_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX90075.1"
                     /translation="MSKKIEPHPLTLKSILENSVRNFGDLPSVSYVDGNPMTYSELGK
                     KVNDTFHLLQSLGLKKGDKVALLGQSMPNWVVAYFSVVSKGLVIVPVLPDFTSLEIEN
                     VLVHSGAKVLFVSDKLISRIENLNTPDLEAVILLNDFSLMSGTVDPKIKVVKTQDDVD
                     VQEDDMAAIIYTSGTTGRSKGVMLTHRNIAFVAMQSYTFQPITSADVFLSLLPLSHTY
                     ENSIGLIYPIIYGASIYYLEKPPTASALLPALAKIRPTMMLSVPLIMEKLFQNQILKK
                     FSENKFKETIYNRPLFRKIIHRIAGKSCIRPSVGDWCFLALAEPNWIAQ"
     gene            complement(2206..2781)
                     /locus_tag="PKH58_13455"
     CDS             complement(2206..2781)
                     /locus_tag="PKH58_13455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNX90076.1"
                     /translation="MNRLATKLGAYHRNKYIDIQNDYFDKGTMSLVGSDKAGMNAKLI
                     LESFRTDSLPIIHIQHIATSPTATFFLPNTKGAEIHDCVKPIGKEKIIIKHYPSSFRE
                     TELLDYLRTKNITDLVICGMMTHMCIDATVRAAKDYGFGVTLIGDACATRDLEINGQI
                     VKAEEVQKSFLAALNYFYATVKTTSDYLNVR"
BASE COUNT          780 a          563 c          532 g          915 t
ORIGIN      
        1 cggggtcgtt ggttcgaatc cgacaagagg ctcaaaacag aaaaagccat tctcatcatg
       61 agagtggctt tttctatggt tgatgtgtta aaaataaatc taatctttgt tttccttatg
      121 ttcacctgca ggatgtttgg tgtacaaaaa ccgcttgatt ttctgagtgg ccgttttttc
      181 caacggttta tcgataagtt ctacccgctt gatctgagaa acacgattaa gtttatcgtt
      241 aatttctttc tttaatatgt gttgtgacca tgttttatat ttatcgtcca acccgtgaat
      301 ggtatgcaaa cctttgacct ttttttcctc gataaccgat ttaatatgtt caatgttttt
      361 ttcgagttct tccagatcaa ggagaatttt agccaccaaa tcgccatcct cttcaaaaac
      421 cagggattct aacacgtatt gattattatt gataaccgat tctatatctt ccggataaat
      481 attctcacca ctggcgccaa taatcgtatt tttcattcta ccctttatat acagacgttt
      541 gtgtgagtcc agatagccat aatcacctgt tcgaaaccaa ccatccggag tcagcacttc
      601 gctggtggct tccggatttt tataataacc aagcatcaca ttggggcctt ttgcccagat
      661 ttctcccaat ccatttttgt ccggattatt aatcttcagt tcaactccct gaacagcaaa
      721 acctgttgag cccagttttg tttgctttgt accggctccg gccagcaatg gagctgattc
      781 ggtgagaccg taaccaatgg cataagggaa atttgcttct ttcaaaaaac gctctactgt
      841 gctatccaat ttggctccgc caatgccaaa aaacaccagt cgcccaccga aggtctgata
      901 caacttttac cggctatgcg gtgtatgatt ttacgaaaca ggggtctgtt atatatcgtt
      961 tccttaaatt tattctccga aaatttcttg agaatttgat tttgaaaaag tttctccata
     1021 atcaatggca ccgaaagcat catcgttggg cgtatttttg ccaaagcagg cagtaatgcc
     1081 gaagcagtgg gaggcttttc gaggtaataa atggaagcac cataaatgat gggataaata
     1141 agcccgatgg agttttcgta ggtatgtgac aggggcagca gtgacagaaa tacatcggcg
     1201 ctggtaatag gctgaaaagt ataggattgc atggctacaa atgcgatgtt tctatgcgtg
     1261 agcatcacac ccttggaacg accggttgtg cccgatgtgt aaatgatggc agccatatca
     1321 tcttcttgta catccacatc atcctgcgtt ttaaccacct taattttggg gtcaacggta
     1381 ccgctcatca atgagaaatc gttgagcagt atgactgctt cgagatcggg agtattcagg
     1441 ttttcgatac gtgaaatgag tttgtcgctc acaaacaaaa ctttagcacc ggagtgaacc
     1501 agaacatttt caatttctaa agaagtgaaa tcaggtaaaa caggaacaat aaccaatcct
     1561 tttgaaacga ctgaaaaata agctaccacc cagttgggca tactttgacc cagaagagcc
     1621 actttatcac ctttttttaa tcctaatgac tgtaaaaggt ggaaagtatc atttactttt
     1681 ttccccaatt cagaataagt catggggttt ccatcgacat atgacactga aggcaaatca
     1741 ccaaaattac ggacagaatt ttcaaggatt gactttaagg tgaggggatg gggctcaatt
     1801 ttttttgaca taaagagatc tttttaagct gagaaatatg tatagcttgc aaagttaatt
     1861 atttacatgg agatattgca caaattattt gaaaatgtgc agtgattaac aaagatttaa
     1921 atagatttcc ttctatttat actgcttgtg aaccggcaat aaagcctaat accgtttgat
     1981 tgatcgttga ttatttcaaa tacctctcat tgatttgtta ttgtaagtgc tttcatttct
     2041 gatacattgc ctgataccgc tgttttttta ttctttactg atagtttgtc tgcttaatcc
     2101 gaaaagtctg atggtaaata gtatcgtgtg ccccggttgc atgaattact tagactacgc
     2161 tacatctaaa gtctcctgcg attccattat tgtaaactgt tgtttttatc tcacattcaa
     2221 ataatcactt gttgttttca cagtcgcgta gaaataattt aacgcggcta aaaagctttt
     2281 ctgaacttcc tcggctttta caatctgtcc gtttatttcc aggtctctgg ttgcacaggc
     2341 atctccaatc agggtgacac caaaaccata atcctttgca gctcttacgg tggcgtcaat
     2401 acacatatgt gtcatcatgc cgcagataac caaatcagtt atattcttag ttcttaaata
     2461 atcaagcaat tcggtttccc tgaaactgct tgggtaatgc ttgatgatta tcttttcctt
     2521 acctattggt tttacacaat catgtatttc cgcccctttt gtatttggta gaaaaaaagt
     2581 ggctgttgga cttgtcgcga tatgttgtat atgaattatc ggcaggctgt ctgttctaaa
     2641 actttcaagg atcaatttcg cgttcatacc tgctttgtct gaaccgacaa gcgacatggt
     2701 tcccttatcg aaataatcat tctgaatgtc aatatactta ttccggtgat atgcacccag
     2761 tttggtggct aaacgattaa tgtttacatg
//
LOCUS       DAOPYB010000310         2740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_51938_length_2740_cov_11.354190, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000310 DAOPYB010000000
VERSION     DAOPYB010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51938_length_2740_cov_11.354190"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKH58_13460"
     CDS             complement(<1..274)
                     /locus_tag="PKH58_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNX90077.1"
                     /translation="MKISSSLKEQIRMLPENPGVYQYFNANEEIIYVGKAKNLKKRVN
                     SYFNKIHDDNKTNVLVKNISYLRYIVVDSEENALLLENNLIKQYQPR"
     gene            complement(399..2273)
                     /gene="mnmG"
                     /locus_tag="PKH58_13465"
     CDS             complement(399..2273)
                     /gene="mnmG"
                     /locus_tag="PKH58_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNX90078.1"
                     /translation="MDFNYDVIVIGAGHAGCEAAAASANMGSKTLLITMDMNKIAQMS
                     CNPAVGGIAKGQIVREIDALGGQMGIVTDKSAIQFRMLNRSKGPAMWSPRSQSDRHKF
                     IHEWTEIVSNTDNLYIWQDTVKELIIDNKQVTGVKTLLGVEMKAKCVVLTAGTFLRGL
                     IHVGKMQIDGGRVSEPASYGLTEQLKSIGFTTDRMKTGTPMRIDGRTIDFSQLTEQVG
                     ENDFHKFSYLKEVKRELPQQSCWITYTNQKTHEILHGGLEFSPLYNGQIKSIGPRYCP
                     SIETKIITFADKNAHQLFLEPEGVNSREWYVNGFSSSLPWQTQIAAIQSVKGLEKVQL
                     YRPGYAIEYDFFDPTQLNHSLETKRIKNLFFAGQVNGTTGYEEAGGQGLIAGINAHIN
                     CHGGENFTLLRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRLLLRQDDADMRLTERS
                     HSIGLAKKNRLDQLTYKKAEQEKLIYFLRNTSIKPREINNFLLSKNSTELVHGCKISD
                     LVLRPQFGITELAQAVPVLREKLESISEISEEVSESVEVMLKYSGYIEREKLIAEKLQ
                     RLEHIIIRDKIDYSTVQSLSTEARQKLARINPETIGQASRIPGISPSDVNILLVMMGR
                     "
BASE COUNT          730 a          562 c          530 g          918 t
ORIGIN      
        1 atctcggttg atactgcttt ataagattat tttcaagcag aagggcgttt tcttcgctgt
       61 caacaacaat atatcgaaga taggaaatgt tttttaccag aacatttgtt ttgttgtcat
      121 cgtgaatttt gttgaaatat gagtttactc tttttttcag gttctttgcc ttacccacat
      181 atattatttc ttcgttggca ttaaaatact gatatactcc cggattttcg gggagcatcc
      241 taatctgttc ttttaatgag gatgaaattt tcatacaacg aaagcgttcc acgtggaaca
      301 aatattaatc aatagattat tttacaatat aatagtttac aaattcaact cagcactttt
      361 gtgcgcaagt tgattatgtt ccacgtggaa catggttatt atcttcccat cataacgagc
      421 aaaatattga cgtcgcttgg agaaattccc ggtattctgc ttgcctgccc aatggtttcg
      481 gggtttattc tggcaagttt ttgccgggct tctgtcgata gtgactgaac ggttgagtag
      541 tcaattttat ccctgatgat gatatgttcg agacgttgaa gcttctctgc gatcagtttt
      601 tcgcgctcaa tatagcctga atatttcaac atcacttcta cgctttcgct gacttcttca
      661 ctgatttcgg agatactttc cagtttttcc cttaatacgg gaacagcctg agccagctcc
      721 gttatcccaa attgaggacg taaaaccagg tcagatattt tgcacccatg tactaattcc
      781 gtagaatttt tagacagtaa gaagttgttt atttcccgtg gttttatgga tgtattgcgt
      841 agaaaataaa ttaatttttc ctgttctgcc tttttgtagg ttagttgatc caaccggttt
      901 ttctttgcca gaccaatgga atgtgagcgt tcggtaagtc gcatatcggc atcgtcctgc
      961 cggagcagta accggtattc ggcacgcgaa gtaaacatac ggtaaggttc gtcaaccccc
     1021 ttggtcacca aatcgtcaat caacacacca atatacgctt cgtcacgcag cagcgtgaaa
     1081 ttttccccac cgtgacagtt gatatgagcg tttatacctg caatcaaacc ttgtccgcct
     1141 gcttcttcat aaccggtggt accattcacc tgaccggcaa agaatagatt ttttattcgc
     1201 tttgtttcga gtgaatggtt tagttgggta ggatcaaaaa agtcgtattc gatagcgtat
     1261 ccgggacgat aaagctgaac tttttccaac ccctttacac tttgtatcgc cgcaatttgt
     1321 gtctgccagg gtagggaaga ggagaacccg ttcacatacc attctcttga attgacacct
     1381 tcgggttcaa gaaataattg gtgagcattt ttgtcggcga aggtaattat ttttgtttcg
     1441 atactcggac agtaacgggg cccgatgctt tttatctgtc cgttgtaaag cggtgagaat
     1501 tccaatccgc cgtgaaggat ttcatgggtt ttttgattgg tataggtaat ccagcaactt
     1561 tgctgaggta gttcacgctt gacttctttc aaatatgaga atttatgaaa atcattctca
     1621 ccaacctgtt cggtcaactg actgaaatcg atcgttcgtc cgtcaatacg catgggagtg
     1681 ccggttttca tccggtcggt tgtgaacccg atggatttta gttgctcggt taagccgtat
     1741 gatgccggtt cagaaacacg tcctccgtca atttgcattt ttccaacatg aatcaatcct
     1801 ctcaggaatg tgcctgctgt caaaaccaca catttggcct tcatttcaac gccaagaagc
     1861 gtttttacac ctgtaacctg tttattatca attattaatt ccttaacagt gtcctgccag
     1921 atatataaat tatcagtatt tgatactatt tcagtccatt catgtatgaa tttgtgtcgg
     1981 tcgctttgag aacgtggact ccacatggct gggcctttcg aacggtttag catgcggaac
     2041 tggatggcac tcttgtcagt tacaattccc atttgtccac ccaaggcatc gatttctctc
     2101 acaatctgac ctttggcaat gccgccaaca gccggattgc agctcatctg tgctatctta
     2161 ttcatatcca tagtgataag cagcgttttt gatcccatgt tggccgaggc ggcagcagct
     2221 tcacatccgg catgtccggc accaatcaca attacatcat agttgaaatc cataaataat
     2281 tcatttagag agtacaaagg taacaagttt cgttgttttg gcaatataac gatactcagc
     2341 gttgattttg cttaacattt tgagtaaaca aaacgaccgg aacaatagta atgtttccgg
     2401 tcgctctgaa tttttagtaa tttattaaac tacggctttg ttttttgcga aaaataaatg
     2461 atagatctga atgagcgcta tcagtccatt cgatacaatc agtggccatt tccatcctaa
     2521 cagtattcca tatagaacaa aaaatgtaca gcctatacaa ttaataattc gaattcttct
     2581 tatctgtttg gggagaaaag aaaccactaa aaaagccatt gctatgtaac caattaattc
     2641 tgttgtttcc atttttcata tttaattggt tgcaaaaata taatattttt tcaaaatttg
     2701 tttaatgttt ttttatggtt accctttatt atttaagaat
//
LOCUS       DAOPYB010000311         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_52012_length_2737_cov_9.407159, whole genome shotgun sequence.
ACCESSION   DAOPYB010000311 DAOPYB010000000
VERSION     DAOPYB010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52012_length_2737_cov_9.407159"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..2557)
                     /locus_tag="PKH58_13470"
     CDS             complement(365..2557)
                     /locus_tag="PKH58_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX90079.1"
                     /translation="MKLNKIFIIAAVLMFAFTANIHAQKARFIKANQLYEAMAYASAL
                     PRYEKVLKKDSTNNEALIKLADCYRRLNNIQQAAKTYEKIISKGIAQPIHKLYYAQAL
                     MELGNYDKAQNYMAEYNADARGETFTKSMNDLDKFFKDTAFYKVETLPFNSDLNDFSP
                     RINKDKLVFASSRKRAYVENHINTWTNNGFFKLYYTRKKANGKYKCVKVFPHSVDNRY
                     NAGPVSFSKQSNTVYVTRNNIIDSKLVRAQDGQAKLQIYAAQLNKKGTAYQYLVDFQY
                     NNKEYNVMHPAISDDGTKFYFVSDMPGGNGGYDIWMCTHENNAWSVPKNLGKSVNTEG
                     DEAFPFVKGSLLYFSSNGLEGIGGLDIYKVSLDDKGMPSGNVYNLGVPLNSMGDDFGV
                     VFDEAGKTGYFSSNRKALNMDDDIYGFIFKKEEKKDILISGVATDKESKELINQTKVT
                     LVDKNNKVIEEVYTNENGNFTFKAQRDQEYTLRGAKEEYINVDKKVLTNTDENTDVVK
                     ADLELEKDPKLKLNIVVTDSRTNAPLNNVVVEVTDKLTGEKYKFVTTNTGEYLGTLDK
                     KIGDKINYTLKFGKDGYYNANSEYSYNITEKGAVVKITQAMDKILEIEPIYFDFDKYN
                     IRPDAAAKLDHLTELLKENPGWYIELYSFTDCRGTEKYNKDLSVERAKATADYLIAKG
                     ITTNRIVGKGMGISKNNNDCDCKKAGKSNCTEEQYQLNRRSEFKLIRK"
BASE COUNT          663 a          555 c          594 g          925 t
ORIGIN      
        1 tttttagttc ggtaaattgc gaactaaaaa aaccgttaaa gttttgttaa agtttgcaaa
       61 actcagagcg tcaggaaaat aaaaaagcta tgattatttt ttatttcaga aatacttttt
      121 tgaaatagaa aaatgtgagt ttttttgaat aataccgata ggaaaaataa tccgccgcgg
      181 cggatagtcc aaacttcgct tcactcgtct gtactattta tttttaccat cggcataacc
      241 aaataagaaa tctcaatatg ccctttggac tatttgatat ttcaatttgg tataaaggca
      301 atacaggata aaaagcaaaa aagccaacca aatggccgac ttttttacaa aataatattt
      361 ttctctattt cctaatcagt ttaaattccg atcttctgtt gagctggtat tgttcttcgg
      421 tgcagttcga cttgccggct tttttgcagt cgcagtcgtt gttattttta gaaatcccca
      481 ttcctttacc cacgatacga tttgtggtga tgcctttagc aataaggtaa tcagctgttg
      541 ccttcgctct ttctacggat aaatccttgt tgtatttttc tgttccgcgg cagtcggtaa
      601 acgaatacag ttcgatatac cagcctgggt tttctttcag gagttcggtc aggtggtcga
      661 gtttggccgc ggcgtcaggc ctgatgttgt atttgtcgaa atcgaaatag atcggctcga
      721 tctcaagaat tttatccatt gcctgagtta tttttactac tgcacctttt tcagtgatgt
      781 tgtatgagta ttcgctgttg gcgttgtagt agccgtcttt tccaaacttg agggtgtaat
      841 taatcttgtc gccgattttt ttatcgagcg tacccagata ttcacccgtg tttgttgtta
      901 caaatttata tttttccccg gtaagcttat cggtaacttc cacgacaaca ttgttcagcg
      961 gagcattggt tcttgagtcg gtcaccacaa tattaagttt cagtttcggg tctttctcta
     1021 attcgaggtc tgcttttacc acatcggtgt tttcatcggt gtttgtaagt acttttttgt
     1081 caacattaat gtactcttct tttgctcccc tgagtgtgta ttcctgatcc cgctgggctt
     1141 tgaatgtaaa gttgccgttc tcgttggtat aaacttcttc tatcaccttg ttattcttgt
     1201 caactaatgt gaccttggtt tggtttatca gctcttttga ttctttgtcg gtggctacac
     1261 cgctgatgag tatatctttt ttctcttctt ttttaaaaat gaatccgtag atgtcatcat
     1321 ccatattcaa cgcctttcgg ttcgaactga aatagcctgt ttttccagct tcatcgaaca
     1381 ccacgccaaa gtcatcaccc atgctgttca ggggcactcc caggttgtac acgttgccac
     1441 tgggcatacc tttatcgtcg agggatactt tgtaaatgtc cagacctccg ataccttcaa
     1501 ggccgttgga agagaaatac agcagactgc cttttacgaa agggaaggct tcgtcgcctt
     1561 cggtgtttac actttttccg aggtttttcg gcaccgacca ggcattattt tcatgggtac
     1621 acatccagat gtcataccca ccgttaccgc cgggcatatc agacacgaaa taaaacttgg
     1681 taccgtcatc gctgatggcc ggatgcatga cgttatattc tttgttgttg tactgaaaat
     1741 ccacaagata ctggtatgcc gtaccttttt tgttgagctg tgcggcatag atctgtagct
     1801 tagcctggcc gtcctgcgcc ctgacaagtt tggagtcaat aatattgttt ctggtaacat
     1861 acaccgtgtt tgactgcttg ctgaaactta ccggtccggc gttgtaacgg ttatcaaccg
     1921 aatggggaaa cactttcacg catttgtatt taccattagc ctttttcctg gtataataaa
     1981 gtttaaagaa accgttgttg gtccaggtgt tgatatggtt ctccacataa gctcttttgc
     2041 gcgaagaagc aaacacaagc ttgtctttgt ttatccgggg tgagaagtcg ttgagatcgg
     2101 agttgaaagg cagggtttcg actttgtaaa aagcggtgtc cttaaaaaat ttgtccagat
     2161 cgttcatgga tttggtaaag gtttcaccgc gggcatcggc attgtattcg gccatataat
     2221 tttgggcttt atcgtaattg ccgagttcca tgagcgcctg agcataatag agcttgtgaa
     2281 tgggctgcgc gatgcctttg ctgatgattt tttcgtaagt ttttgccgct tgctgtatgt
     2341 tgttgagcct gcggtagcag tcggcgagtt ttatcagggc ttcgttgttg gtgctgtcct
     2401 ttttgaggac tttctcatag cgaggtaggg cgctggcgta tgccatggcc tcgtaaagct
     2461 gattagcctt gataaatctc gctttctgtg cgtggatgtt tgccgtaaag gcaaacatca
     2521 gcacagcagc aatgatgaat attttattta gtttcataat ttgaaaaatt atgttttaaa
     2581 gctgacgact agaaatacct tgggttagca actttcctgc ctttataata gaacaggtag
     2641 gagagggcga tctcatgcga accgctggaa accgtattca gcttcgacat ggaataatcg
     2701 tacgaatagc tgataagaaa ctggggcacg acataca
//
LOCUS       DAOPYB010000312         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_52105_length_2734_cov_10.082867, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000312 DAOPYB010000000
VERSION     DAOPYB010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52105_length_2734_cov_10.082867"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKH58_13475"
     CDS             <1..200
                     /locus_tag="PKH58_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90080.1"
                     /translation="RKSDYRALSGRRVIQIYNDVALIVEKFFQRGNMDAETRISGCRR
                     LSEGWVFDNNQALAADQKKWN"
     gene            331..810
                     /locus_tag="PKH58_13480"
     CDS             331..810
                     /locus_tag="PKH58_13480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX90081.1"
                     /translation="MTRDDFIICIKLYTPNLLRFSKRVAGNVIEAEDIVQECFEILWK
                     NQEKVEINSAKSYLFSIAHKKIIDTFRTDRKMQDFESASEELVTKPDEADTEQLVRLA
                     INQIPALYRELITLRDLEGYSYKEIESITGLSEARVKVTLFRARKQIKDRILQLEVS"
     gene            807..1616
                     /locus_tag="PKH58_13485"
     CDS             807..1616
                     /locus_tag="PKH58_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90082.1"
                     /translation="MTLKIDINNYEEIMFRLLEDDFDTPTRIDLLKQIENDDLFKFEW
                     ESWQKTRIIDPLENYSGESRELTDKIMLMADRHPVAKKRIVYYLAAASVLVLMACFTL
                     FTVDFNSGKKQEIAGAFEKPPVPEIKSSPVIENSPVPAVRSDGKNPEKPKLSREVLPV
                     VDDSDTIVLIGNHLAEIPVAFDTVRAETSEAAKVLYKKPRYTVTTRTMLLVDYDRQND
                     DPAQKGKIKLSKLLTNTRLILQRKPDGEPERIILMGEDNNSLCININYTEK"
     gene            1613..2512
                     /locus_tag="PKH58_13490"
     CDS             1613..2512
                     /locus_tag="PKH58_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90083.1"
                     /translation="MKTNCKLLMLIFGLASFQSMAQKEDSVQIIYDNQKTIIPVPAMG
                     SRTSVNYADSLEVVQIDVARVKPGDISHLSYYSSNNLTPDKPLKKVKWYSQIEAGYNL
                     KFSPYHESYVFYYIGTQQFRVYYNTNNSHGYNLGITILEKERCITNRFSYISGFKLAF
                     AQSFRKGKGPYPRSTNPSDTIILNSYVDYEASTNYSLQFLFPFGFRRYIGPEKLNARI
                     NFGTNIGSSQGITKSSLYNRTITQFSPSSLFLQPYFGFEKGKFGILTSGEVLFGSRFK
                     IAERSTVLNVQFGIGASLTYRFF"
BASE COUNT          859 a          538 c          616 g          721 t
ORIGIN      
        1 aacgaaagtc agattaccgg gccctgagtg ggaggagagt aatacaaatt tacaacgatg
       61 ttgccttgat tgttgagaaa ttttttcaga gggggaatat ggatgcagaa acccggattt
      121 caggctgccg gcgactgtct gagggctggg tgtttgacaa taatcaggct ttggctgcgg
      181 atcagaaaaa gtggaattag gacaatcttt atggacaaat gatattttat gaaatgccat
      241 tgtgtaattc aggatagctg aaaaggcact atctcagaaa cattttttaa attgtgtaac
      301 tttttcatcg aaaatccgat ttaacagtat atgacgagag acgatttcat tatctgtatt
      361 aagctttata ctccaaacct tctcagattc tcgaagcggg tggcaggtaa tgtgatcgaa
      421 gcggaagaca tcgttcagga atgtttcgag atactatgga agaatcagga gaaagtggaa
      481 ataaattcag caaagtcgta tcttttttcc atagcgcaca aaaaaatcat cgatacattc
      541 cggacagaca ggaaaatgca ggattttgaa tcagcctccg aagaacttgt tacaaaaccg
      601 gatgaagccg atacggaaca gttagtccgc ctggccataa accagattcc tgcattatac
      661 agggaactga ttacgctccg cgacctggaa ggatactcat acaaggagat tgaatccatc
      721 accggtctgt ccgaagcccg cgtcaaggta acccttttca gggccaggaa acagatcaag
      781 gatcgaatat tacaactgga agtatcatga cactgaagat cgacatcaac aattacgaag
      841 aaatcatgtt ccgcctgctg gaggatgatt ttgatacgcc aacccggatt gatttgttga
      901 aacagataga gaatgacgac ctgtttaaat ttgagtggga atcgtggcag aaaacaagaa
      961 taatcgaccc gctcgagaat tattccggcg agagccggga gctgacggat aaaatcatgc
     1021 tgatggcaga tcgtcatccg gttgcaaaaa aaagaatcgt ttactacctg gctgccgcgt
     1081 ccgtccttgt gctgatggct tgttttactt tattcaccgt tgatttcaat tccggaaaga
     1141 aacaggagat agccggggct tttgaaaaac ctcctgtccc ggaaataaaa agttccccag
     1201 ttattgaaaa ttcaccggta ccggcggtta ggagtgacgg gaaaaatcca gaaaaaccaa
     1261 agctttccag ggaagtgtta cccgttgttg atgattccga cactatcgta ctgatcggaa
     1321 atcacctggc ggaaattccg gtggcgtttg atacggttcg ggctgaaacc agcgaagctg
     1381 caaaagttct gtataaaaag ccaagatata ccgttacaac caggactatg ctgttggttg
     1441 actacgaccg gcaaaacgat gatcctgcac agaaagggaa aataaagctg agcaagctgc
     1501 tgaccaatac ccgcttgatc ttacagcgga aacctgatgg cgaaccggaa aggattatcc
     1561 ttatgggaga agataataat tcattatgta ttaatataaa ctatacagaa aaatgaaaac
     1621 aaactgcaaa ttattaatgc tgatttttgg attggcctca tttcagtcga tggcgcagaa
     1681 ggaagactct gtgcaaataa tctatgataa ccaaaagaca atcatcccgg tgccggcaat
     1741 gggcagccga acttcggtaa attacgccga ttcattagag gtcgttcaga ttgatgttgc
     1801 gcgggtaaaa ccgggggata tttctcactt gtcatattat tcttcaaaca acctgactcc
     1861 ggacaagcct ttgaaaaagg tgaaatggta ttcgcagatc gaagccgggt ataacctgaa
     1921 attcagtccc tatcatgaga gctacgtatt ctattacata ggtacacagc aatttagagt
     1981 atattataat acgaataata gccatggata taacctgggt atcaccattt tagaaaagga
     2041 acgttgtatc acaaaccgat tttcatatat cagtggtttt aaactggcct ttgctcaatc
     2101 attccgtaag gggaaaggcc catatccgcg gtcaactaat ccaagtgata ctattatact
     2161 taactcatat gttgattacg aggcatcaac aaactacagt ctgcagtttt tatttccatt
     2221 cggtttcagg cggtatatcg ggccggaaaa actgaatgca agaattaatt ttgggacaaa
     2281 tattggctca tcacaaggca ttactaagtc gtcactttat aacagaacaa ttacgcaatt
     2341 tagtccctcc tctttatttc ttcaacccta ttttggtttt gaaaagggga aattcggcat
     2401 tctcacatca ggggaagtat tattcggctc aagatttaag atcgccgaac gaagtaccgt
     2461 cctgaatgta caatttggga ttggagcttc attgacatac cggttttttt aatcccctgc
     2521 cacgatgccg tatcggttat gaaggatttt tggcgaaatc tgccatgcgt ccggtctttt
     2581 gaagatcatg gtaattcagc gagagttcga aatgatacag aagttaaata ataagatgag
     2641 cagttcacaa ataattcgtg attgcataac aacctgacgc cttagcggca ggaaatgaag
     2701 atcagggaat cagcgttcaa cccctattgc aggt
//
LOCUS       DAOPYB010000313         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_52237_length_2729_cov_10.299925, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000313 DAOPYB010000000
VERSION     DAOPYB010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52237_length_2729_cov_10.299925"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PKH58_13495"
     CDS             <1..623
                     /locus_tag="PKH58_13495"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90084.1"
                     /translation="TVMLGNTILKPETTEAGTGYFKEEIPVEMLKPEMGKITVGNKGS
                     AGIGWGAAYWQYFQDQDKIKSQGKEMNISKKLFVERVENKQRTMLPVEQTVLKKGDKV
                     ITRLVITTDRDLEFVALKDLRAACFEPVNQLSGCVWKEGTSYYQTTKDASTQFFFSFL
                     PKGTYVFEYELWVNNTGTFTSGISSVQCLYAPEFAAHTGGEKLMVK"
     gene            787..2226
                     /locus_tag="PKH58_13500"
     CDS             787..2226
                     /locus_tag="PKH58_13500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNX90085.1"
                     /translation="MLFNSFDFAVFMPIVFLLYWLVFNRTVTLRNSFLLVASYAFYSF
                     WDWRFLFLLLFSSLADFILGLQLHKTSDDQPRKRKMWLYCSLAINFGILGFFKYYNFF
                     TESFVEAFTIFGRELNYEPLKIILPVGVSFYTFQSLTYIIGIYKRKVEPTNRIIDFLA
                     YVSFFPQLVAGPIERAAHFLPQFHAPKVFSYKNCQTGLLLIAFGLFKKIVIADRLAVY
                     VNGVYGDIPSAGGISILVAVLFFAFQLYFDFSAYSDIAIGSARLLGFELMTNFRRPYL
                     SKSFSEFWTRWHISLSSWFRDYVYFPLGGSRRGTRRTMINVMIVFLLSGLWHGASWNF
                     VIWGGVNGLFLIIFDRIFKLEQPGNIKKYLAPAFIFCSWAVSLIFFRAQTFADAISVF
                     THAGFAGSDLIYNFGLKAPELKFAFFMLAASMLYELLAEKKGEKLAVWFYNKPVIVRW
                     SLYLAIVFSIIYLGSYGSSNDNTFIYFQF"
     gene            2231..>2729
                     /locus_tag="PKH58_13505"
     CDS             2231..>2729
                     /locus_tag="PKH58_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90086.1"
                     /translation="MAQQNNNKTRKKPAAAPVKQSVKTAQTQVKPQKPQTIYTLLAKA
                     AIFFLVVVAVVKFTDLKGYFNPDYTNDHTRRKWNAFYQFTNDKPVDVVLVGNSHLYTG
                     INPENLSTALGANCFILASPGTTMTDVYYCLKEAIAVSKPKVAVVETFGINDYTSHEL
                     KQGTLS"
BASE COUNT          737 a          589 c          603 g          800 t
ORIGIN      
        1 caactgtaat gctgggcaac acaatcctga aacccgaaac taccgaagcc ggaaccggtt
       61 atttcaaaga agaaattccg gtggaaatgc tgaaaccaga aatggggaaa attaccgtgg
      121 gaaataaagg ttcagccgga attggatggg gagcagctta ctggcagtat tttcaggatc
      181 aggacaagat taagtcgcag ggcaaggaaa tgaatatcag caaaaaactt tttgtggaaa
      241 gagtggagaa taaacaaaga acaatgctgc ccgtggaaca aaccgtcctg aaaaaaggcg
      301 ataaagtgat tacccgactg gtaatcacca ccgaccgcga tctggaattt gtagcactca
      361 aagacctgcg ggctgcctgc ttcgaaccgg tcaaccagct ttcgggatgc gtttggaaag
      421 aaggtacaag ttattaccag accaccaaag atgcatccac ccagttcttt ttcagtttcc
      481 tgcccaaggg aacctatgtg tttgaatacg aactgtgggt gaacaataca ggaactttca
      541 ccagtggaat aagctctgtg cagtgtttgt atgcacccga attcgcggca catacaggtg
      601 gtgaaaaatt aatggtgaaa taagaacaga tagttgaaaa aacagaaacc ggatgctcct
      661 ctgaacatcc ggtttctgtt tcgacacgta tgatttgatg agaaaaaatt atatctttgt
      721 gcctgcaaaa tcaggctttt attcggataa ttatcaccgt caaatagagg ctataatcaa
      781 ttaactatgc ttttcaattc gtttgacttc gcggttttta tgccgattgt ttttttgctg
      841 tactggcttg tttttaaccg tacagttact ttacgtaatt cctttttgct ggttgcaagt
      901 tatgcctttt acagtttttg ggattggagg ttcctgtttt tgctgctttt cagctcgctg
      961 gccgatttta ttctgggtct tcagttgcac aaaacatcag acgatcaacc ccgaaaacgg
     1021 aaaatgtggc tctattgcag tctggcaatc aactttggaa ttcttggctt tttcaagtac
     1081 tataatttct ttaccgaaag ttttgttgag gcatttacga tatttggtcg cgaactgaat
     1141 tacgaaccgc ttaaaattat tcttccggta ggggtgagtt tttacacctt ccagtccctg
     1201 acctatatca tcgggatata caaacgtaaa gtggaaccta cgaaccgaat tattgatttc
     1261 ctcgcgtacg taagtttttt ccctcagctg gtagccgggc caatagaacg ggcagctcat
     1321 ttcctgccac agtttcatgc tccaaaggta ttcagctata aaaactgtca gacaggatta
     1381 ctgctcatcg cttttggctt gttcaaaaag attgtcattg ccgaccgact agctgtttat
     1441 gtaaacggag tgtatggtga tataccttcg gccggaggaa tttcaatcct tgtcgcggtt
     1501 ttgttttttg cttttcaact atatttcgac ttttcggctt attccgatat tgccatcggt
     1561 tcagcccgtc tgctgggttt tgaattgatg accaacttcc ggagacccta tctttccaaa
     1621 tctttttccg aattctggac acgctggcat atatctcttt caagctggtt cagggattac
     1681 gtgtatttcc cgctgggtgg aagccgtcgg ggtacccgcc gcaccatgat taatgtgatg
     1741 atcgttttcc tgttaagcgg tttgtggcac ggtgcatcct ggaatttcgt tatttgggga
     1801 ggtgtcaacg gcttattcct gattatcttc gaccggatat tcaaactcga acaacccggg
     1861 aatatcaaaa aatacctggc tccggctttt attttctgca gttgggcagt atcgctcatt
     1921 ttcttcaggg cgcagacatt tgccgatgcc atttcggttt tcactcatgc tggctttgcg
     1981 ggaagtgatt tgatttacaa ttttggcctg aaagcgccgg aattaaaatt cgcttttttc
     2041 atgctggcag ccagtatgct ctatgaacta ctggctgaga agaaaggcga aaaacttgcg
     2101 gtgtggtttt acaacaaacc ggtgattgtt cgctggagct tatatctggc catcgttttc
     2161 agtattattt acctgggctc gtacggctca tccaacgaca acacgtttat ctatttccag
     2221 ttctaaagtc atggcacaac aaaataataa taaaacccgg aaaaaacctg cagctgcacc
     2281 ggttaaacag tcagtaaaaa ctgctcaaac acaggtgaag ccacaaaaac cacaaaccat
     2341 atatactctc ttggctaagg cagccatatt ttttctggtg gtggtagctg tagtgaagtt
     2401 taccgacctg aaaggctatt ttaatcccga ttacaccaac gaccatacac gccgtaagtg
     2461 gaacgccttt tatcagttta caaatgataa acccgtggat gtggtactgg tcggaaattc
     2521 gcatctctat accggcataa atcccgagaa tttatccact gcgctcggag ccaactgctt
     2581 tattctggct tcccccggaa cgaccatgac ggatgtttat tactgcctga aagaagcaat
     2641 cgctgtatca aagccaaaag tagctgtggt cgaaacgttt ggtatcaatg attatacatc
     2701 gcacgaactt aagcagggaa cgctgtccg
//
LOCUS       DAOPYB010000314         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_52441_length_2722_cov_11.693663, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000314 DAOPYB010000000
VERSION     DAOPYB010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52441_length_2722_cov_11.693663"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..433)
                     /locus_tag="PKH58_13510"
     CDS             complement(107..433)
                     /locus_tag="PKH58_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90087.1"
                     /translation="MKTNRLLLTVFSGLLLISANVNAFTYYITFTGSGASTAVDSVIA
                     FNLSKGTQVKIPAGVTLQLYDVENSVENLNTTSDLAAVFPNPVTDKATFTSSFASSWL
                     LHKNTL"
     gene            complement(881..2191)
                     /locus_tag="PKH58_13515"
     CDS             complement(881..2191)
                     /locus_tag="PKH58_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90088.1"
                     /translation="MKKIFTLLALLLTYTLLFAVTIPVPQKMSYQAVIRNSSNALVTN
                     HLVGIKISILQTSATGTVVYSETQTPTSNANGLINIAIGTGVIVSGTFSAIDWSAGPY
                     FIKTEVDPAGGTAYSITATSELLSVPYAQLSKDVQNKQWTESGSAIYFNTGKVGIGKN
                     PGADARQFQVLTTDYQAVAAVNNSATYASIYASNQGEGPAADFRNKIRIADGTQAAGK
                     VLTSDANGYTSWLVPDYTQWTTAGNNIYYTTGNVGIGTATPGVYKLYVKDAGNVYTGM
                     FENNGSSVTLVSRNYGGGPAGNFISNGTTYTLYAQNTSTGPAAYFDGTLKVAGGNTSE
                     INRSQTGDANTVSICYGSVEASGTKNTGGSTANFTVTKISTGVYDITITDETYSQSTH
                     CAIASLGDPGFINTNAVGGKLRVYTYSTSNVAQDREFSFVVYKP"
     gene            complement(2197..2691)
                     /locus_tag="PKH58_13520"
     CDS             complement(2197..2691)
                     /locus_tag="PKH58_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90089.1"
                     /translation="MSKRMLIILLLLLVCGFATLTAQVLHAGITAAGGNASGTGGSVS
                     YSVGQLFYTTAFGTNGSVSEGVQQPFEISVLSGMEDITGIDLYYSVYPNPTIGKLTLN
                     LKNYEPNTFTYQLYDVNGKMLRNDRLIENETIIEMSDLSSATYFLKVIMKNKEMKLFK
                     IIKN"
BASE COUNT          761 a          551 c          535 g          875 t
ORIGIN      
        1 tgggtgaaga aatacaaaag gcatttcctc agtcgaaagt ggtgaaaaca ttaaacacca
       61 tgtggtgcgg acttatggta aacccctcaa tggtaaacaa tggcgatcat aacgtattct
      121 tatgcagcaa ccacgaagat gcaaaagaag aagtgaatgt agctttatca gtcacaggat
      181 tgggaaacac agcagccaaa tcagaagttg tgtttaagtt ctcaacggag ttttccacat
      241 cgtaaagttg aagtgtaaca ccggcaggaa ttttcacctg cgtaccttta ctcaggttaa
      301 acgcaattac actatccaca gcggtgcttg cgccggaacc ggtgaaagtg atgtagtaag
      361 tgaatgcatt cacattggca gaaatcagta acaatcccga aaacacggtt aataaaagtc
      421 tgtttgtttt catgatacga tttttatatt taaattaatt tttgagaatt cattttgata
      481 tatcaatcct tcacacaacg aactgaacgc actcattttt taaaataatt tgcatgcaac
      541 aggcaattgt tattgattat gccaatcttt atccccctta gattatcggt tacgagtgat
      601 tgactactgt tacagataac tgtctggtag ttcattttca atgggaccgg tagtggcgat
      661 gcaaataaca ctgagttgaa aactaacacg acaacttatg taaataagtt tctcatgata
      721 attggtttta tttttttgtt ttttctgttt tggtatgctt tatttctagg tttgcaggca
      781 atacttacac acaataaact ttagaaatga ttatttttca tttcattggg aatgtttttt
      841 cgggttttgt gccgaatacg aacttgagtg acagactgaa ttaaggttta tataccacaa
      901 aactgaattc cctgtcttgt gctacattag atgtactgta agtatatact ctcaattttc
      961 cgccaactgc attcgtgttg ataaatcccg gatcacccag actggctatg gcacaatgag
     1021 tagactgact gtaagtttca tctgtaattg tgatatcata aactcctgtt gatatttttg
     1081 ttactgtaaa attagctgtg ctaccacctg tgttttttgt accactagct tctactgaac
     1141 cgtaacaaat tgatacggta ttagcatcac ctgtctggct gcggtttatt tctgaagtat
     1201 taccacctgc taccttaagc gtaccatcaa agtaagctgc cggacctgta cttgtgtttt
     1261 gtgcatagag ggtataagtg gttccattac ttataaaatt tcctgccgga cctccaccat
     1321 agtttcggga taccagggtg acagaactcc cattgttctc aaacatacct gtatatacat
     1381 ttcctgcgtc ttttacatag agtttgtaaa cgccgggagt agcagttcca atgccgacat
     1441 tccctgtagt gtaatatata ttattccctg cagtagtcca ttgggtataa tcaggtacta
     1501 accaggacgt atagccattt gcatctgaag tcagtacttt gcccgcagct tgtgttccat
     1561 cagcaattct tattttgttt ctgaaatcag cggctggtcc ttccccctga ttactcgcat
     1621 aaatggatgc ataggtggca ctattgttga ccgcagccac agcctgataa tccgtggtca
     1681 gtacctgaaa ctgccgggca tcagctccgg gattttttcc tatacccact ttgcctgtat
     1741 tgaaataaat tgcagagccc gattctgtcc actgcttatt ttgtacatct ttggaaagct
     1801 gagcatacgg cacgctcagc aattcactgg tagcagtaat gctgtaagcc gttccgccag
     1861 ccgggtcaac ttctgtttta ataaagtagg gtcctgccga ccaatcgata gcagagaaag
     1921 ttccacttac aattactcct gtgcctatag caatatttat aagtccattg gcattgcttg
     1981 tcggggtttg tgtttcggaa tataccactg taccggtagc tgatgtttga agaatgctta
     2041 tctttattcc caccagatga ttggtaacga gtgcattgct gctgttgcgg atgactgcct
     2101 gataactcat tttctgagga acaggtattg ttactgcgaa taataatgta taagtcaata
     2161 acagggctaa tagtgtaaat atttttttca tgacatttag tttttgatga ttttgaacaa
     2221 tttcatttct ttatttttca taattacttt cagaaaatag gtcgctgaac ttagatcaga
     2281 catttcaata atggtttcat tttcaatcag tctgtcgttc cggagcattt taccattgac
     2341 atcatacagt tgatatgtga acgtgtttgg ttcataattt ttcagattta gcgtcagttt
     2401 gccgatggta ggattgggat atacggaata gtaaagatct atacctgtaa tatcctccat
     2461 acccgacaaa accgaaatct cgaacggctg ctgcacacct tccgataccg agccattggt
     2521 tccgaatgcg gtagtgtaaa acagttgacc caccgaatag cttaccgagc ctccggtgcc
     2581 acttgcattt cctccagctg ccgtaatacc agcatgaaga acctgtgctg tcagtgtggc
     2641 gaacccacat accaacagta acagtagaat aatcaacatt cttttggaca tggtctttgt
     2701 tttaagggtt aaacattaaa ta
//
LOCUS       DAOPYB010000315         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_52444_length_2722_cov_9.635546, whole genome shotgun sequence.
ACCESSION   DAOPYB010000315 DAOPYB010000000
VERSION     DAOPYB010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52444_length_2722_cov_9.635546"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1073)
                     /locus_tag="PKH58_13525"
     CDS             complement(6..1073)
                     /locus_tag="PKH58_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HNX90090.1"
                     /translation="MIKTGDIVRFLNAVGGGKVTRFDEKKNMVYVEDADGFEIPALAH
                     ECVIIQAVNEKTNFPVKDFSAKSNNATVSPSKEVKMEEPKNPEPIVETTEGEIFNAFL
                     AFFPKDIKRLETSGYECYLVNDSNYFLYYNFVAGENGNRRSIANGTIEPNMQEFISEI
                     EKSQLNDWEKVRVQIIPFKKGKTYTEQNVLDFTLRLNTVKFYKLHSFTETDYFDDLCM
                     LISLTEENQKKQLTEISPSEIKQALQQKAQPQLRPKIIQKQPKNEIIEVDLHINALID
                     NTAGLSNADMIGYQMETFHRILEEYKKKKGQKIVFIHGKGEGVLKNEIEKQLKSRYKN
                     YLYQDASFREYGFGATMVTIK"
     gene            1158..2399
                     /locus_tag="PKH58_13530"
     CDS             1158..2399
                     /locus_tag="PKH58_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNX90091.1"
                     /translation="MNHPEQKEAIYIDDFNYELPDERIAKYPLSKRDSSRLLLYRDGV
                     VSESVFSNLAQYLPQNCLLVCNNTRVIQARLVFHKKTGARIEVFCLEPLIPSDYALSL
                     SATGSCEWKCLIGNLKKWKEGALQQKILLNEKEVTFSAELLETEENTHRIRFSWDADE
                     FSFSEILMQIGELPIPPYLHRKTEETDKKTYQTVYSKIEGSVAAPTAGLHFTPEVFAS
                     LKSKNIEIEELTLHVGAGTFQPVKSATINQHTMHIETIAVPLNTIKHIQSKIGNIIAV
                     GTTSVRTLESLYYIGKNLLYLPDENITDFRVDQWEPYETTEDIPVEKSLQNIIDYLEK
                     YDLDTLHAETQILIQPGFQFRVITGMITNFHQPKSTLLLLVSAFVDGNWRTIYDYALS
                     NGFRFLSYGDSSLLMKFRKAD"
     gene            2470..>2722
                     /locus_tag="PKH58_13535"
     CDS             2470..>2722
                     /locus_tag="PKH58_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HNX90092.1"
                     /translation="MEKIIILDFGSQTTQLIGRRLRELNEYCEILPYNKFPENTDDIK
                     GVILSGSPYSVYDPTAFKADLSRIRGIFPVLGICYGAQFM"
BASE COUNT          734 a          558 c          542 g          888 t
ORIGIN      
        1 atttattact taattgttac catcgttgcg ccaaaaccat attccctgaa tgatgcatcc
       61 tgatacagat agtttttata ccggctttta agttgttttt ctatttcgtt tttcagaaca
      121 ccttctcctt ttccgtgtat aaacacaatt ttctgtcctt tctttttctt atattcttcc
      181 aatatgcgat ggaatgtctc catctgatag ccaatcatat cggcattcga caatccggca
      241 gtattgtcaa tcagagcatt gatatgcagg tccacttcga taatttcgtt tttaggctgc
      301 ttctgaataa ttttagggcg tagctgcggc tgggcttttt gctgtaaagc ttgtttaatt
      361 tcggacggcg atatttcagt caattgcttt ttctggtttt cttcagtcag actgatgagc
      421 atgcagaggt catcgaaata atcggtttca gtgaaactgt gcaatttgta gaatttcacg
      481 gtattcagcc tgagtgtgaa atcgagtaca ttttgttcgg tgtacgtttt accctttttg
      541 aacgggatta tctgtacgcg cactttctcc caatcgttca gttgtgattt ttcaatttcg
      601 ctgataaatt cctgcatatt cggttcgata gttccgttag ctatgcttct gcggttaccg
      661 ttttctccgg ctacaaagtt gtagtaaaga aaataattgc tgtcgttcac caggtaacat
      721 tcgtaaccgc tggtttccaa acgttttatg tcttttggga aaaatgccag gaatgcattg
      781 aaaatttcac cttctgtggt ttcaacgatt ggttccggat tttttggttc ttccattttc
      841 acctctttcg acgggcttac tgttgcattg ttgcttttcg cggagaaatc ctttaccgga
      901 aaattggttt tttcgtttac cgcctgaatg atcacacact cgtgggcaag tgcaggaatt
      961 tcaaatccgt cggcgtcttc tacatacacc atattctttt tctcgtcaaa acgggttact
     1021 tttccacctc ctactgcgtt cagaaaacgc acaatatcac cggtttttat cataatcttt
     1081 atttttattg cctattaact tggcacttgg ttttaattgt tttaattttg cgcaaattta
     1141 caaactttta ttgccttgtg aaccatcccg aacaaaaaga agctatttat attgatgatt
     1201 ttaactacga attgcccgat gaacggatag ccaaatatcc gctttcaaag agagattcgt
     1261 cccggttgct cctttaccga gatggggtgg tatctgaaag tgtcttctca aatctggcgc
     1321 aatatctgcc tcagaattgt ctgcttgtgt gcaacaatac aagggtcatt caggcaaggc
     1381 tcgtttttca caaaaagaca ggagcccgca ttgaggtgtt ttgcctggaa ccgctgattc
     1441 catcggatta tgccttgtcc ttgtcggcaa ctggcagttg tgaatggaaa tgcctgatcg
     1501 gaaacttaaa aaagtggaaa gaaggtgcct tacaacagaa gatactttta aatgaaaagg
     1561 aagtgacttt ttctgctgaa ctgttggaaa cggaagaaaa tactcaccgc atccgtttca
     1621 gctgggatgc cgacgaattc agcttttccg aaatcctgat gcaaatcggc gaactgccca
     1681 ttccgcctta tttacaccgg aaaaccgaag aaaccgacaa aaaaacatat caaacagtct
     1741 attctaaaat tgaaggttcg gtggcggcac ctacggcagg gctgcatttc acaccagagg
     1801 tgtttgcaag tctgaagtcg aaaaatattg aaatagaaga gctcaccctg catgttggag
     1861 ccggaacttt ccaaccggtg aaatcagcaa caattaatca gcataccatg catatcgaaa
     1921 ccattgcagt gccgctgaat acgattaaac atattcagag caaaatcggt aacatcattg
     1981 cagtgggtac tacttcagtg cgtactctgg agagtttgta ttatattgga aaaaatctcc
     2041 tgtatttacc cgatgaaaac attacggact tcagagttga tcagtgggag ccttatgaaa
     2101 cgaccgagga tattccggtt gaaaaatctt tgcagaatat tattgattat ctggaaaaat
     2161 atgacctgga tacgcttcat gccgaaacac aaatcctgat acagccgggc tttcaatttc
     2221 gcgttatcac cggaatgata acaaacttcc atcagcctaa aagtacgtta ttgctgctgg
     2281 tatctgcctt tgtcgatggt aactggcgaa cgatttacga ttacgcccta tcaaacggat
     2341 ttcgctttct gagttatggt gacagttcgc tgttaatgaa atttcgaaaa gccgattaat
     2401 cttaaatcat ttctgacttt ctttcttttt tgttattttt gcaaaaacat taatttgaaa
     2461 aagatcttca tggaaaaaat tatcatcctt gattttggtt ctcaaaccac acaactaatt
     2521 ggtcgccgtt tgcgcgaact gaatgaatat tgtgaaatcc ttccttataa taagtttccg
     2581 gaaaatacgg atgatattaa aggagtaatc ctctcaggaa gtccctattc tgtgtatgat
     2641 ccaactgcat ttaaggcaga tctgagccgg ataagaggca tctttcctgt gctgggtatc
     2701 tgttatggcg ctcagtttat gg
//
LOCUS       DAOPYB010000316         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_52681_length_2714_cov_8.610756, whole genome shotgun sequence.
ACCESSION   DAOPYB010000316 DAOPYB010000000
VERSION     DAOPYB010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52681_length_2714_cov_8.610756"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..>2714
                     /locus_tag="PKH58_13540"
     CDS             387..>2714
                     /locus_tag="PKH58_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX90093.1"
                     /translation="MIKSVNTDLAKRVVKELQETNELDAVYIEYPCPIQPKYEFNSNF
                     QWYLQTTNNGIDVQRAWTINKGRSDVIIAVCDGGVDYTHPNLDPGDRSRIITGYDTGN
                     EDNDPMDDLPDSPDSYANHGTHIAGIIGANPTSTHNISGVMQNCKIMPVKMVGSGGIK
                     IPFSSVYLWDFSTTAFPSDVADAIDYAVNNGANVINLSFGFSFPNVPLIDVLSGLNVL
                     VQSVERAYQNNVVIVASMGNEALEGNPRNFPAYFSQVIAVGNTTANKVKASTSNTASY
                     ISVCAPGTNILSTVRNNSTDWKSGTSMAAPVVSGLAGLIISQGKDRGFNLTNDDIRQI
                     LERTADDVGDVGFDNETGYGIVNAFNALSLLDLPNSLVHGNSYGGGSVKLQMIDKWIV
                     SSSIWGLAAGTYLNVDQYEVTKRINFDKPFINPPIVWLRERESVSLSFEIQNNLRPFV
                     QISNITNTGFDVRYAAYYIRYNSVGQTLNKWVPSNIPTTKIAYTAVGIPAPSISGPSS
                     ICSQATYKIDNLPVGATIQWSVNIPYVTLQPGQDSVIIQKTGSGFINLTASIFINGAL
                     ITTIVKDDILVGGVAGLGLSSYSNLQMLDDGSYYKILPSSDVWSYRGSLTVVASGSNL
                     NYSWSETYASSNFIQWSANGSTVDVYSKYDNLNLTLKCTASNSCGSMNRYYSFTSSTN
                     PILPESIILSPNPASGYVNVTVSDEEVPAEKTLSAVSLKSSSNYQIQLWNSSGLIKTV
                     QTDQKKYQLDLSGLSPGFYYVLVIRDGKTYRRQLMV"
BASE COUNT          921 a          462 c          510 g          821 t
ORIGIN      
        1 aaatataatt tacagtaaaa gtaattacaa aaaatatgct tcatactact tgtggaaaat
       61 aaacattgaa actaaaaata aaattcagtt aacacaatga tacaaatacc aatatgaaaa
      121 aaatattatg tatgatactg ttatgtttta cggctaattt attttgccaa aaacaaacag
      181 aaaattcaga agtaagagtt ttgctaaatt ttagtaacag tctattgaca aatcaatata
      241 aatccgtatc aatagattca atacgctcgt tgaaaataca aaaaatttta tctaatttag
      301 gtattactga cttaacagca gtctttaaga atagatacat ttctagaaat tttattgcag
      361 ataataaatc atcgtcaaac tgacgaatga taaaatctgt aaatacggat ttagccaaaa
      421 gagttgttaa agaattacag gaaacaaatg aattagatgc tgtgtatatt gaatatccct
      481 gtccgattca acctaaatat gaatttaatt ctaatttcca atggtaccta caaaccacta
      541 acaatggtat agatgtacag agagcttgga ctataaacaa aggaagaagt gatgttatca
      601 tagcagtatg tgatggtgga gtagattata cgcatccgaa tctagaccct ggagacagaa
      661 gcaggataat aaccggatat gatacaggaa acgaagacaa tgaccctatg gatgacttac
      721 ccgatagtcc agatagttat gcaaatcatg gaacacatat agctggtatc atcggagcta
      781 atccaacttc aacacataat atatccggag taatgcagaa ttgtaaaata atgccggtta
      841 aaatggtagg ttccggaggt ataaagatac ctttttcatc cgtttattta tgggactttt
      901 ctacaacggc atttccatct gatgttgctg atgcgattga ctatgctgta aacaacggtg
      961 ctaacgtaat aaatttaagt tttggctttt catttcctaa cgtaccacta attgatgtct
     1021 taagtggctt gaatgtactt gtacaatcag tagaaagagc ctatcaaaac aatgtagtta
     1081 tagtagcttc aatggggaat gaagcgcttg aaggtaatcc gagaaacttc cctgcctatt
     1141 ttagtcaagt tattgctgtc ggaaatacaa ctgcaaataa agtaaaagct tcgacatcaa
     1201 atactgcatc ttatattagt gtttgtgctc cgggaactaa tattttatcg actgtaagaa
     1261 ataattcaac agactggaaa tctgggacat ctatggctgc gccagttgta tcaggtttag
     1321 ccggattaat aatatcacaa ggaaaagaca gaggatttaa tttaactaat gatgatattc
     1381 gtcaaatatt agaacgaacg gcagatgacg taggggatgt aggatttgat aatgaaacag
     1441 gctatggaat agtgaatgct tttaatgctt tatctctttt ggatttacca aattcgcttg
     1501 tgcatggaaa ttcttatggt ggaggaagtg taaaattaca gatgattgac aaatggattg
     1561 tttcaagttc tatatgggga ttagcagcag gaacttatct taatgttgac caatacgaag
     1621 ttacgaaacg aattaatttt gataaaccat ttataaatcc tccaatcgta tggttaaggg
     1681 agagagaaag tgtttctctt agtttcgaaa ttcagaataa tttacgtcct tttgttcaaa
     1741 tttctaacat aacaaataca ggttttgatg tccgctatgc agcatattat atcagatata
     1801 attcagtagg tcaaacatta aacaagtggg ttccatcaaa tataccaaca acaaaaatag
     1861 catatactgc cgttggaata cctgcacctt ccatctccgg tccctcttcc atttgctctc
     1921 aagcaaccta caagattgat aatttgccgg tgggcgctac aatacagtgg agtgtaaata
     1981 tcccttatgt gacattacaa cccgggcagg acagtgtgat aatacaaaag accggttccg
     2041 gctttatcaa tctcactgct tccatattta ttaacggcgc tttgattaca accattgtaa
     2101 aagatgatat tttagtagga ggagttgccg gattgggatt aagcagctat tccaacttgc
     2161 agatgcttga tgatgggagt tattataaaa ttctgccttc ttcagatgtc tggagttaca
     2221 gaggaagtct taccgtagtt gcttcaggtt cgaacctgaa ttattcctgg tcggagacat
     2281 acgcttcatc caactttatt cagtggtcgg ctaatggctc tacggtggat gtttatagta
     2341 aatatgataa cttgaacctg actcttaaat gtacggcaag taattcttgt ggttccatga
     2401 acagatacta ttccttcact tcaagtacta atcccatcct gcccgaatct atcattctta
     2461 gtccaaatcc tgcatctggt tatgtaaatg tcacagtttc cgatgaggag gtaccggcag
     2521 aaaaaacatt atcagcagta tcacttaaat cttcatctaa ttatcaaatc caactctgga
     2581 acagttccgg tctgataaaa acagtacaaa ccgaccaaaa gaagtatcag ctggatttga
     2641 gcggcttatc acccgggttt tactatgtgc tggttatcag ggatggaaaa acttaccgca
     2701 ggcagctgat ggtg
//
LOCUS       DAOPYB010000317         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_52701_length_2713_cov_10.462754, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000317 DAOPYB010000000
VERSION     DAOPYB010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52701_length_2713_cov_10.462754"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(512..2425)
                     /locus_tag="PKH58_13545"
     CDS             complement(512..2425)
                     /locus_tag="PKH58_13545"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90094.1"
                     /translation="MNRFVIIVFTGLTLLLRTAFCQNADSLLTRKDSIKVVAFCISKI
                     DTTTSPQINAANVDTNIHYFQNYDPIRKKYVFNANIGNTGQASENMVFETDPSCGFSY
                     GLKSFDAYKFNMSEAKNYYSLLPFTNLFWTLGAKREQMFQVTHYHHVQNKVFMGVDYR
                     IVNAPGRDNFRQKTNDHALSIFTYYATKNKKYGVSINYIFNKIKTQENGGITDDTAYI
                     NFRKGDNASPVDIHLKTAENNWRESTVLLKQYVTLSRENAYKRNDSLRPLKTFNLGRV
                     LHTFCFTKQRFKFTDDNPNLSYYPLIPNDTGSLNDSVWFYKMDNTLEWTNSELNKENK
                     NRMLRYFVQLRHAYTEVNQVNGKFYISNLIPSLGLSVVPYKNMKINLSGEYVLLDYNQ
                     GDLSLNASAKQHFIFNEKNYGSVQLKFNYSKTKPEWFYEHFSSKYFQWDYDFAKQQTI
                     YTGLNYSYRNLSVGLDYYNLFNYVYMGVDLRPRQYTGGTINVLSAYVYKNFVIGKFQI
                     DNKVVYQYTDKPGLIRKPQLIAMQSYMFTTPMFKKALLFQVGIDLLYNTKYYADSYQP
                     AIGTFVLQNEYKAGNYLNADVFINLKVKRLRAFLKLSNFLSGLIGYNYFTVPHYPMQD
                     RLFRFGISWRFHD"
BASE COUNT          846 a          493 c          532 g          842 t
ORIGIN      
        1 tttatataat ttattaacaa tcaattgttt acatctatta ttaacgaagt gttgtttata
       61 tacatacgat taaagttaaa acgtggtgct gaaggaatta ttgcttctct ccctatcaag
      121 ctatctcaca gaaatgtagg gtcagatgag agccagcaac cgtttcaact tgaggttagc
      181 tatgttctat ttggccaatt ttcgacttgc tcgatgagta gtcaaagtaa aaaattgttt
      241 tatcgctatt aaaaaatagg atttatggct aataaaatat caattagtgt ctgtccataa
      301 agtcagagtt tgaatcagaa tgttcgaatt cgagacctgc ccgcctgatc cgccagctgg
      361 gggatcagac ggggatgaag gcggacatta tggacagaca ctaattatca aagatttgtg
      421 ttgaagtgta tataataaga tgattgaacc tttcaataaa aacctaaaag ttacgacaat
      481 aatcgaattt tttcagtact tcaggcactt tttagtcgtg gaacctccag cttattccga
      541 atctgaacag ccggtcctgc ataggataat gcggtacagt gaaataatta taaccaataa
      601 gtccgcttaa aaaattggat aattttaaaa atgccctcag ccgtttcact ttcaggttga
      661 tgaatacatc cgcattcagg taatttccgg ccttatattc gttctgaagg acaaatgtac
      721 caatagcggg ctgataacta tcggcatagt acttcgtatt atacaacaga tctattccta
      781 cctgaaacaa taaagctttc ttgaacatcg gggtggtaaa catatacgac tgcatggcaa
      841 taagctgagg tttcctgatt aagccgggtt tatcggtata ttgataaact actttattat
      901 cgatctggaa cttaccgata acgaaattct tgtaaacata agcagaaagc acattgatgg
      961 ttccgccggt atactgccgt ggccgcaaat ccacacccat gtatacatag ttgaacaggt
     1021 tgtaataatc cagtcccacg cttaaattcc tgtacgaata atttagtccg gtatatatgg
     1081 tttgctgctt tgcaaaatca taatcccatt gaaaatattt tgaactgaaa tgctcgtaaa
     1141 accattcagg cttagtttta ctgtaattga acttcaactg cacagaacca taattttttt
     1201 cattgaaaat aaaatgttgt tttgcagaag cattcaatga aagatcaccc tggttataat
     1261 ccaaaagaac atactcgccc gaaaggttta ttttcatatt cttatatggc accacactca
     1321 ggcccaatga cgggatcagg tttgaaatat aaaatttacc attgacctga ttcacctcag
     1381 tataagcatg tctgagctga acaaaatagc ggagcatccg gtttttattt tctttattta
     1441 gctcagaatt agtccattcc agcgtattgt ccattttata aaaccataca gaatcattca
     1501 acgaaccggt atcatttggt atcagcggat aataactaag attgggatta tcatcggtaa
     1561 atttaaaacg ctgttttgta aaacaaaaag tatgcaacac acggcctaag ttgaatgttt
     1621 tcaaaggcct gaggctgtca tttctcttgt atgcattttc acgcgaaaga gtaacatatt
     1681 gtttaagaag aaccgtcgat tcgcgccagt tgttctctgc tgttttcaga tgaatatcca
     1741 cagggcttgc attatctccc ttgcggaagt taatataggc tgtatcatca gttatcccgc
     1801 cattttcctg tgtcttaatc ttgttgaaga tatagttgat agaaacaccg tattttttgt
     1861 tttttgtagc ataatatgtg aagattgaca aggcatggtc gttggttttc tggcgaaaat
     1921 tatcgcggcc gggagcattc acgatgcggt aatccactcc cataaacact ttgttttgca
     1981 catgatgata atgggtaacc tgaaacatct gttcgcgttt cgcgccaagg gtccagaata
     2041 agttcgtgaa aggtaagagg gaataataat ttttcgcttc tgacatgttg aacttatagg
     2101 catcaaaaga tttcaagcca taagaaaatc cgcaggaggg atctgtttca aataccatgt
     2161 tttcggacgc ctggcccgta tttccaatat tggcattgaa cacatatttt ttgcgtatcg
     2221 ggtcgtagtt ctggaagtag tgaatattgg tatctacgtt tgctgcgttg atttgcggcg
     2281 atgtggtggt atcgattttg ctgatgcaga aagcgaccac cttaatagag tcttttcttg
     2341 tcaaaaggga gtcagcattc tggcaaaaag ctgtccgaag taagagggta agtccagtaa
     2401 atacaattat aacaaaacgg ttcatttgtt atttcttaaa gggcgtaaag gtaaaaaata
     2461 aacgttaaag aacattatta aaaaatgaaa aagtggccac aacaagcaaa taatgatcat
     2521 cagtcagggc aagaaaaatc tgttttcgca gtggggttaa ttaaagtggc agcgacatat
     2581 gatgatagtg ttagcgatcc gccagttggc ggaaagcgtt acactatcac atcccgataa
     2641 gattattaca aatttttgtc acagccatcc gaactttttg agaaaataac attgattatt
     2701 tctgtatccc ttg
//
LOCUS       DAOPYB010000318         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_53375_length_2690_cov_8.562808, whole genome shotgun sequence.
ACCESSION   DAOPYB010000318 DAOPYB010000000
VERSION     DAOPYB010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53375_length_2690_cov_8.562808"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKH58_13550"
     CDS             complement(<1..283)
                     /locus_tag="PKH58_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HNX90095.1"
                     /translation="MKKPLLLFWLILLSFASPAQKFDKLALTPPMGWNSWNKFACDID
                     EKLIMDIADQLVKTGLRDAGYIYINLDDCWHGQRDSQGFITADPVKFPSG"
     gene            complement(401..1291)
                     /locus_tag="PKH58_13555"
     CDS             complement(401..1291)
                     /locus_tag="PKH58_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX90096.1"
                     /translation="MNNKPHHIITEITPLSDKDCFYIADRLKSEFTYPIHSHSEFELN
                     YVENAKGVRRIIGDSVEVIGDYDLTLVAGDKLEHVWEQYQCTSRNIREITIQFSSELF
                     FGNLLNKNQFDSIRKMFENAKQGVSFPMEAIMKIYPQLDTLASETNGFYAVIKFMTIL
                     YELSMSDNSRILSSSSFANIDDSSDSRRISKVHDYINIHFKEPIRLEDLASIAGMSSV
                     SFSRFFKLRTGKTITDYLIEIRLGYASRLLVDTTNSISEICYECGFNNLSNFNRIFKK
                     KKDCSPKEFRENYRKTKQII"
     gene            complement(1561..2334)
                     /gene="yaaA"
                     /locus_tag="PKH58_13560"
     CDS             complement(1561..2334)
                     /gene="yaaA"
                     /locus_tag="PKH58_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNX90097.1"
                     /translation="MLILLSPAKIQNFKPQNQVQENTQPVYLKEAEKIIGELKTYSIQ
                     ELAGLIQVNAKIAEQNADRFFHWHTPFTPDNAKQALWVYNGEVFRGLDAGSMSKQEID
                     YAQEHLRILSGLYGVLRPLDLIQPYRLDVSNQLSPEFGRDLYDFWKERISREVRKTLD
                     KTEKPGIILNLMSNEYFRTLDHSILKTRIIDVEFLQYQPHTDRFKPIVIYIKKARGLM
                     ARFVIRHEINDPESLKAFSEEGYWYNEQLSTDNKLVFVR"
BASE COUNT          774 a          510 c          551 g          855 t
ORIGIN      
        1 tgcccgaagg gaattttaca gggtcggccg tgataaatcc ctgactgtcg cgctgaccgt
       61 gccagcagtc gtcgagatta atgtaaatat agccggcatc gcgtaatcct gtttttacca
      121 actggtcggc aatgtccatg ataagttttt catcaatatc acaggcaaat ttattccagc
      181 tgttccagcc cataggcggt gtcagcgcca gtttgtcaaa cttttgagca ggagaagcaa
      241 aagacagcaa tattaaccaa aaaagtaaaa gcggtttttt catgatttca aaatgtattg
      301 gttaaatgaa ggaaaatgaa tgttgcaaaa atagaacttg gaacaatcaa aaaccactcg
      361 tatattatca ataactaata caattttatc atgagtgatt ttaaatgatt tgttttgttt
      421 ttcggtaatt ttccctaaac tctttgggcg aacaatcttt tttctttttg aatatgcggt
      481 tgaaattcga aaggttattg aagccacatt cataacatat ttccgaaatg gaattggtgg
      541 tatccaccag cagtctgctg gcatatccga gtcggatttc aatcaggtaa tcggtgattg
      601 ttttgccggt tcgcaactta aaaaaacggc tgaatgaaac cgacgacatc cctgcaatgc
      661 ttgccagatc ttccaaacgg atgggctctt tgaaatggat attgatgtaa tcgtgtactt
      721 tggaaatacg gcggctgtcg gaactgtcgt caatgttggc aaacgacgaa ctggaaagta
      781 ttcgcgaatt gtcgctcatt gataattcat acaggattgt catgaatttg atgaccgcgt
      841 aaaatccatt tgtctccgaa gccagcgtat ccagctgcgg atatattttc atgatggctt
      901 ccattggaaa actaaccccc tgtttggcat tctcaaacat cttccggatg ctgtcgaact
      961 gatttttatt cagcaggtta ccaaaaaata attccgatga gaactgtatg gttatttcgc
     1021 gtatatttct ggaagtacac tgatattgtt cccagacatg ctcgagttta tcgcctgcta
     1081 cgagcgtcag atcatagtca ccgattactt ccacagaatc accgatgatt cgccttacac
     1141 ctttggcatt ttccacatag ttgagttcaa actccgagtg tgaatgaatg gggtaggtga
     1201 attctgattt taacctgtct gcaatgtaaa aacagtcctt atccgacagc ggagttattt
     1261 cagtaatgat gtggtggggt ttgttgttca taaagtatca tatcacgata ataataatac
     1321 aaacataggt attattttgt tgtatccgat atgaaaaatt gttattttaa taaaatagtt
     1381 gatattcaat gtaaaatcag gctaatgtcc gatagaaagg tttttttgct gatcagaata
     1441 ctgttgtcat cagaaatctt ataaagccgg atatttgtta tcccagtatc ataatattat
     1501 tgtatatcga gcatattttc gaagaaatgt catcaacctc tgtagggaaa accgtcaata
     1561 ttaccgtacg aacaccagtt tattgtccgt agagagttgt tcattgtacc agtagccttc
     1621 ttcagagaat gctttgagcg attccgggtc attaatttca tgccggatta caaaacgtgc
     1681 cattaagccg cgtgctttct tgatatagat gacgatgggt ttgaacctgt cggtgtgtgg
     1741 ctgatattgc agaaactcca catcgatgat gcgggttttc aatatggaat gatcaagtgt
     1801 acggaagtat tcgttggaca tcaggttgag aatgataccc ggcttttccg tcttgtcaag
     1861 ggtttttctc acttcgcggc ttatccgttc tttccagaaa tcgtataaat cgcgtccgaa
     1921 ttcgggactg agctgattgc tgacatccag acggtatggt tgtatcagat ctaacggtcg
     1981 cagcactccg tacaaacccg aaagaatacg caggtgttcc tgtgcatagt caatctcctg
     2041 tttgctcatg ctgccggcat ccaaacctcg aaagacctcg ccgttgtaca cccacagagc
     2101 ctgcttggcg ttgtcggggg taaatggggt gtgccagtgg aaaaaacggt cggcattctg
     2161 ttcggcgatt ttagcattca cctgtatcag tccggccaac tcttgaattg aatatgtctt
     2221 caactcaccg ataatttttt cagcttcttt cagatataca ggctgggtat tttcctgtac
     2281 ctgattctga ggcttgaaat tctgaatttt agcaggtgag agtagtataa gcatgattct
     2341 tttttattaa atgatacaaa gataaaaaaa agtgcaataa gaaagtcctt caaaataatt
     2401 atgtgtgttt ttaattaatg ttcagtctga gaagatatca aattcatcat tacagtaata
     2461 aattaacatg cttaaatgct cttttactcc ttcttcaggg cgttaggtgg ctttatactt
     2521 tcaaaaaaat cgtgtcggca gaagcttatt ccggatttat ttagtaattt tgtaacttca
     2581 aaagttaatt tgtcaacaca ttgacataca gatactttca aatttattaa tactcaaaat
     2641 acttatctga tgaaacctac attatttgta cttgcagccg gaatgggcag
//
LOCUS       DAOPYB010000319         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_54016_length_2671_cov_7.165520, whole genome shotgun sequence.
ACCESSION   DAOPYB010000319 DAOPYB010000000
VERSION     DAOPYB010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54016_length_2671_cov_7.165520"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKH58_13565"
     CDS             complement(<1..295)
                     /locus_tag="PKH58_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90098.1"
                     /translation="MKYSTLSKSLMAKILVFIAICAVPFVANYFDNNRDLKRKQYERH
                     LLDIARSAAAKDMCEKSGEAEADSPDMAALQEFFMTADPALDRVPAERLFAAKQ"
     gene            718..1272
                     /locus_tag="PKH58_13570"
     CDS             718..1272
                     /locus_tag="PKH58_13570"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90099.1"
                     /translation="MNQLYISMGEYSIPISEAEYRLSNPGSLADSVYAAVELANIFLR
                     CRNESVSSGNNCSSIYQPLLPADYEAFRQYKTRLTDLLYPKPSDFTATLPTNEPEIQF
                     TVSPNPAQNFISITLSRSVNTPMTIQLIEAAGKVIFSHAICCTSLIEEIKLDVSNIAS
                     GGYYFLRLTTGNKIIGTKPIVILK"
     gene            1328..2551
                     /locus_tag="PKH58_13575"
     CDS             1328..2551
                     /locus_tag="PKH58_13575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90100.1"
                     /translation="MKTLNTALAILIWIVLMPLDNYSQPNLMNRGHANDDIYVIYDGG
                     TYNYLLYLTSNGEVISKRSDNVLYVITGEPIPGKLFGHTSAYFGVSEDYGQHFNMLPP
                     LPYYYITIFGLLGGEVAGNYTIYGEDISVYPYPMGVLYKTTDNFATYTLVADTLNELT
                     YCEGGSVAGELYQIPILNGHASLCHSLDYGATFDTLAIDTAIINENLGIEVKKLSHGA
                     AQGELYLVTIEEIAASKYKYIIYHSADYGVSWQMKYFFEDYSNKLSFTAGRGACKFYI
                     AIVTTTPGSTYNTLTILYSDDCAEAFAPYYHLLSPDVGINENDVLPSVLSVSPNPASE
                     IATITYKLRKPGNVQLEIFSPEGRKVKVINEGYKARGKNSCQLISSNLSPGIYNIVLK
                     MEREVLTSTRFVVIR"
BASE COUNT          806 a          575 c          517 g          773 t
ORIGIN      
        1 cctgctttgc agcaaaaagc ctttcggccg gcaccctgtc caatgccgga tcggcggtca
       61 tgaaaaattc ctggagagca gccatatcag ggctgtcggc ctctgcttca ccggattttt
      121 cgcacatgtc tttcgcggca gcgctccggg ctatatccaa caggtgccgt tcatattgtt
      181 ttctcttcag gtcgcggttg ttgtcaaagt agtttgccac gaaaggcact gcgcatattg
      241 caatgaaaac aaggatttta gccatcaaag atttagagag ggtgctgtac ttcataacga
      301 gttattattt gagcataaat ttcggcaaat aatagatatc tctataaagt aaatcaatct
      361 gatgatactt ctggctgaag aatctcttta aactgttttt gctaaccact tattggcgct
      421 tgagaatatt tggcacgata cataagagca gaggaatctc ttacaatgcg aaaggatcac
      481 tttcaatgag tagagttccc acggtggatg gggaatatat tgaatttaag tgttttgtaa
      541 caactcaaaa aaatattcta cactgtgatg aatctgaaaa attatttcgt atattaacaa
      601 ccttataaac ctaataacat tttatattat gaaacttctt ctcagcttca atcactcctg
      661 actgatattg ctcacaacca ggaaaataac atcctgggtg aaacggcaaa agcccttatg
      721 aatcaattat atatcagtat gggagaatat agtattccga tcagtgaagc ggaatacagg
      781 ttatccaacc ccggttcact agccgattcg gtgtatgcag cagttgaact cgcgaatatt
      841 tttcttagat gcaggaacga atcggtatca tccggcaaca actgttcaag tatctatcag
      901 cctttattac ccgcagatta tgaggctttc cggcagtata aaacccgatt gactgacctg
      961 ctgtatccga aaccatctga ttttacagcc acactaccca caaatgaacc tgaaattcaa
     1021 tttactgtaa gccccaatcc ggctcagaat ttcatttcaa taaccttatc ccgctcggtt
     1081 aacactccta tgaccataca actcattgaa gcggcaggga aagtaatttt ttctcatgca
     1141 atttgttgta cttctcttat tgaagaaata aaactggatg tatccaatat agcttctggt
     1201 ggatattatt ttctgcgttt gactaccggt aataaaataa ttggcacaaa accaatagta
     1261 atattaaaat aaaaacctac cgttcaaaat tcagtaaatc aacaatcaat cataattcca
     1321 tcaacttatg aaaacactga acacagcatt agcaatttta atttggattg ttctgatgcc
     1381 tctcgataat tacagtcagc caaatcttat gaacagagga cacgccaatg atgatatata
     1441 tgtaatttac gatggcggca catacaacta tctgttatac ctcacttcga acggtgaggt
     1501 gattagtaaa cggagtgata atgttttgta tgtgattact ggagagccaa taccaggcaa
     1561 actatttggt catacaagcg cctatttcgg cgtaagtgaa gattacgggc aacacttcaa
     1621 tatgttgcct ccattacctt attattatat tacgattttt ggccttttag gcggcgaagt
     1681 tgcgggcaac tatacaatat atggtgagga tatttctgtt tatccttacc ctatgggagt
     1741 gttatacaaa accactgata actttgccac ttataccctt gttgcagata ctctgaatga
     1801 actaacatat tgcgaagggg gctccgttgc aggagaactt taccaaatcc cgattttaaa
     1861 cggccatgca tccctgtgcc atagcctcga ttatggcgcc acctttgata ctcttgccat
     1921 tgatactgcc atcataaacg aaaacctggg tattgaggtg aaaaagctaa gccacggagc
     1981 cgcgcagggc gagttgtacc ttgttaccat tgaggagatt gccgcctcaa agtacaagta
     2041 tatcatttac cactcggcag attacggcgt atcgtggcag atgaagtact tttttgaaga
     2101 ttattctaac aagctttcgt ttactgcagg ccggggagcg tgtaaattct atatagcgat
     2161 agtcaccacc acccccggat caacttataa tacccttact attctttata gtgacgattg
     2221 cgccgaagca tttgcccctt attatcacct tctttcaccc gatgtcggca tcaatgaaaa
     2281 tgacgtactg ccttccgttc tgtcggtttc accgaatccg gcaagcgaaa ttgcaacaat
     2341 aacatataaa ttgaggaaac ccggcaatgt tcagcttgaa atattttcgc cggaaggcag
     2401 gaaggttaaa gtcatcaacg aaggctacaa agcccgggga aagaacagtt gtcaactcat
     2461 tagcagcaat ctgtcaccgg gcatatataa catagtcctt aagatggaac gggaagtatt
     2521 gacttcaacg agatttgtcg taataaggta gattatcaga cctccttaca aaatacgccc
     2581 atgctcgcgc gcgtctcctg acgcgtgcgc cataactact catgtgacta tttcaaaata
     2641 atttttatat ccgagcaatt aatatgtgta a
//
LOCUS       DAOPYB010000320         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_54224_length_2664_cov_7.003833, whole genome shotgun sequence.
ACCESSION   DAOPYB010000320 DAOPYB010000000
VERSION     DAOPYB010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54224_length_2664_cov_7.003833"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..2506
                     /locus_tag="PKH58_13580"
     CDS             200..2506
                     /locus_tag="PKH58_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNX90101.1"
                     /translation="MNYDEFLMSKEKRIQPKGKLIELKEISPVLFDFQKDIVKWAVKM
                     GRCAVFADTGLGKTFIQLEWGRLIGGNILIFAPLSISKQTIREGKKINVIVKYIKEED
                     EFEPGICITNYENIEKFQTIAKKLNGVILDESSILKSIDGKIRLKLIKYFSSTVNYKL
                     CCTATPSPNDYTELGNHAEFLNVCKTTEMLSMFFVNGNKTGETVTDSGIVIRKKYSNK
                     NGTEWRLRYHAQNEFFRWLSSWAMSIRKPSDLGYNDDGYILPKLNIIPDVIDFKYQPS
                     DELFFTGLKGLGQRAEIRRDSSGLKLDRIKEIINGSNEQWLIWCGLDRESSIAKKALE
                     NSIEVKGTDEPDFKAQAFEDFQDNKIKILITKSKIGGHGMNFQNCHNIIFFGLNDSWE
                     MFYQSIRRCYRFGQNKEVNVHIVISDIETSIFENIVKKGEMAERMMNGLINEVKKYEM
                     EELGKDVKRINVEYKEETVTSEYYTAMLGDSCKRMSEVESESVGLSVYSPPFADLFTY
                     SATNRDLGNSTSWREFYKHYKFIVQELLRVTQSGRVSCVHTSDIPAMQMKDGYIGIKD
                     FPGLVIRLHQLCGWIFYGRAIVTKNPQSQAIRTKSKALLFTQLRKDSSDSRPALLDHI
                     LIFKKPGETKIPVRPVENGEMDNETWIDWAGGIWTGIHESDTLQYSAARDKDDEKHIC
                     PLQLGTIERCIKLYSNPGETILTPFGGIGSEGYQAIKFGRKAILIELKQSYFKILIQN
                     MKNIEYETKKPDLFTDQVNNSADLSLSL"
BASE COUNT          946 a          425 c          541 g          752 t
ORIGIN      
        1 ccctgcaaac tcatcgaccg cccctgtgac ggttgtaaaa tctgtcaggc cgttcactcc
       61 cggccccagg tagagaagat gaaaacgaag cggataaatc gtaaggactt tttaaagata
      121 agccggtaaa tgtcaatcgt ttcgttgata tatatcattg cttttcaatt tatttcacta
      181 taattttact gaaaaaacta tgaattacga tgaattttta atgtcaaagg aaaaacgcat
      241 tcagcccaaa ggaaagctaa ttgaactgaa agagataagt cctgttctat ttgattttca
      301 aaaagatatt gttaaatggg ctgttaagat gggaaggtgt gctgtatttg cagatacagg
      361 tttggggaaa acatttattc aacttgaatg gggtaggtta atcggtggta atattttaat
      421 ttttgcccca ttgtcaatat caaaacagac aatcagggag ggtaaaaaaa taaatgtcat
      481 tgtaaaatat atcaaggaag aggatgagtt tgaacctggt atctgtataa caaattatga
      541 gaatattgaa aaattccaga ccattgccaa aaagttaaac ggggttatat tagatgaatc
      601 aagtatttta aagtccattg acggtaaaat taggttaaag ctgataaaat acttttcatc
      661 aaccgtaaat tataaacttt gctgcaccgc tacaccatcc ccgaatgatt atactgaact
      721 tggtaaccat gcagaatttc tgaatgtttg taagacaaca gaaatgcttt ccatgttctt
      781 tgtgaacggg aataaaacag gcgaaacggt cacagattca ggaatagtta tacggaaaaa
      841 gtacagcaat aaaaacggaa cagaatggag gttgagatac catgctcaaa atgaattttt
      901 ccgctggtta tcatcatggg ctatgtcgat acgcaaacca tctgatcttg gatataatga
      961 tgatggttat atattgccaa aattgaatat cattcctgat gtcattgatt ttaaatatca
     1021 accgtccgat gaattatttt ttaccgggtt aaaaggatta ggacaacggg cagaaattcg
     1081 cagagattca tccgggttaa aacttgatcg gataaaggaa ataattaacg gcagtaacga
     1141 acaatggctg atatggtgtg ggcttgatcg ggaatcttcc attgcaaaaa aagcactaga
     1201 aaacagcatc gaagtaaaag ggactgacga acctgatttt aaagcacagg catttgagga
     1261 ttttcaggat aataaaataa aaatactgat cactaaaagt aaaatcggcg gccacggcat
     1321 gaattttcaa aactgccata atattatatt ttttggctta aatgattcat gggaaatgtt
     1381 ttaccaatca attcgccggt gttaccggtt tggtcaaaac aaagaggtaa atgttcatat
     1441 cgtaatttca gatattgaaa cttcgatatt tgaaaatatt gtaaaaaaag gcgaaatggc
     1501 cgaacgaatg atgaacggat taattaacga agtaaaaaaa tatgaaatgg aagaattagg
     1561 taaagatgtt aaaagaataa acgtcgaata taaagaggaa acagttacat ctgaatatta
     1621 tacagccatg cttggtgatt catgtaaaag aatgtcagag gttgaatctg aatcagtggg
     1681 gctatcggtt tattcccctc cgtttgctga tttgtttaca tattcagcaa ctaatcgtga
     1741 tttagggaac tcaacaagct ggcgtgaatt ttacaagcat tataaattta ttgtacagga
     1801 acttttgagg gttactcaat caggtagggt tagttgcgta catacgtccg atataccagc
     1861 aatgcaaatg aaagacggat atatcggaat aaaggacttt ccaggattag taatcaggct
     1921 tcatcagctt tgtggctgga tattttacgg tagggctatt gtaacaaaga atccacaatc
     1981 acaagcaatt agaacaaagt ctaaagcatt attatttaca caattacgca aagattcatc
     2041 agattctcgc ccggcattac ttgaccatat tttaatattt aaaaaacctg gtgaaacaaa
     2101 aatacctgtt aggcctgtgg aaaatggaga aatggataac gaaacatgga tcgattgggc
     2161 tggaggtatt tggaccggaa tacatgaatc agatacgctt caatattcag ctgccaggga
     2221 taaggatgat gaaaaacata tctgcccgct ccagcttggg actattgaaa ggtgcattaa
     2281 attatattca aatcctgggg aaactatctt aactccattt ggaggaatag gtagtgaggg
     2341 gtatcaggct ataaagtttg gaaggaaagc gatattaatc gaattaaaac aaagttattt
     2401 caaaatatta attcaaaata tgaaaaatat tgaatacgaa acaaaaaaac ctgatttatt
     2461 tacagatcaa gtaaacaatt ccgccgattt atccttatct ttgtaatcaa gaagcctgac
     2521 aaccttcatc caatgatagc acaatcaaac atttacgcta acaacgaatc ccgcaccggg
     2581 ggaaagtgcg gtcaaaggtg cgctatcaca tccgagatcg ctggagccgt caactccgat
     2641 cctgggcggg ttttcttttt atga
//
LOCUS       DAOPYB010000321         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_54946_length_2642_cov_8.506378, whole genome shotgun sequence.
ACCESSION   DAOPYB010000321 DAOPYB010000000
VERSION     DAOPYB010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54946_length_2642_cov_8.506378"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKH58_13585"
     CDS             <1..310
                     /locus_tag="PKH58_13585"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HNX90102.1"
                     /translation="CGPVCSSYIHFYDKGWNKLKMDLPKLTNRDWIKNPDEIVNGVNV
                     ADMFPASFIELRFDTKNNGIEVRNNSAEYLSGEDKKVIEPLMKKDIVKLKWNNGTWER
                     "
     gene            362..1024
                     /locus_tag="PKH58_13590"
     CDS             362..1024
                     /locus_tag="PKH58_13590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX90103.1"
                     /translation="MSSQSDQLKSNGGYFYNPRPEMLKYISDSARSILEVGCGEGSFV
                     KTLKRKDREIWGVEIYSEAAEKAKAVCDFVLTGSFDAVFEQLPEKHFDCIIFNDVLEH
                     MYAPWDVIMQVKSLLSDNGVVVSSIPNFRYISNLILEIVWKGEFRYKPEGGILDDTHI
                     RFFTSKSIQRMYREQGYEIVLHEGIKPCKSWKEKLFIFLSFGILKDARFKQFATVAKI
                     KK"
     gene            1024..2016
                     /locus_tag="PKH58_13595"
     CDS             1024..2016
                     /locus_tag="PKH58_13595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX90104.1"
                     /translation="MPPKVSIIIPVYNAGGYLVRCLDSVVSQTLKDIEIILVLDNPTD
                     GSDLVAEQYSQKDNRIKIIKNENNLHIGCSRNKGLEIAQGEYVGFADHDDYCQPEMFE
                     MLYKNAVEFQADVVVSDYCDEKQNGIDYFGFPTGLTNEEFKVRLFDALISGRQSQPNI
                     RTFDNVNVIWNQLYKQEFLVNYNINFSDNRILTMEDVLFNIKVHHYAKQVRYIPEAFY
                     HHITNGQNEFQNYSYRAIDKVMPHLVEVFDFLVKEDLYQHFKKSFAICTLKRLYTSYR
                     NEVRFKGILASFGFFEKVRSHSKLREILNNFKDNTLWKNELALTKRAFLMIVSK"
     gene            2045..>2642
                     /locus_tag="PKH58_13600"
     CDS             2045..>2642
                     /locus_tag="PKH58_13600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX90105.1"
                     /translation="MKSGNVLVTVVIPVFNTAEYLNEAIGSILRQTLQKLEIVAIDDC
                     SSDNSLQILRELAAADSRIKVIAMPQNQGQSVCRNKGLEIAKGEYIYFFDSDDILEED
                     CLQLCYEKCTELQTDLLLFDGKSFTENGISLEFNPKYDRTGFFTKSVSTGLETIETMY
                     KNKAYSCSVCLIFIRKSYLDQTGLRFYPGVFYEDVLFSM"
BASE COUNT          874 a          406 c          564 g          798 t
ORIGIN      
        1 gtgcggaccg gtctgcagtt cttatattca tttctacgac aagggatgga ataaattaaa
       61 aatggattta cccaaactca caaatcgcga ttggataaaa aatccggatg aaatagtgaa
      121 cggggtaaat gtggcggata tgttccccgc atcattcatt gaactgagat ttgataccaa
      181 aaataatggg atagaagtcc gtaacaacag tgctgaatac ctgagtggag aagataaaaa
      241 ggtgatagag ccattgatga aaaaggatat tgttaagctt aaatggaata atggaacatg
      301 ggaaaggtga gtttttctaa taatttgatg agaagtatca attaattcaa agcataaacc
      361 aatgtcaagt caatcagacc agttaaaatc aaatggtggt tatttttaca atccacgccc
      421 tgaaatgttg aaatatattt ccgatagtgc gaggtcaatt ttagaagtgg gctgtggcga
      481 aggcagtttt gtcaaaacct taaagcgcaa agatcgcgaa atctggggcg tggaaatata
      541 ttcagaagct gctgaaaaag cgaaagcggt ttgtgatttt gtactgactg gcagttttga
      601 tgcagtattc gaacaattac cggaaaaaca ttttgattgt ataattttca atgacgtttt
      661 agaacatatg tatgctccct gggatgtaat catgcaggta aaaagcctgc tcagtgataa
      721 tggtgtagta gtgagctcaa ttcccaattt caggtatatt tcaaacttaa ttttggaaat
      781 agtctggaaa ggtgagttta ggtataaacc cgaaggagga atactggacg atacacatat
      841 ccgttttttt acatcaaaaa gtatacagag aatgtatcgc gagcaagggt acgaaattgt
      901 attgcacgag ggcatcaaac cctgcaaaag ctggaaggaa aaattgttta tttttctttc
      961 tttcgggata ttgaaagatg ctcggtttaa acaatttgca acagtagcta aaatcaaaaa
     1021 ataatgccac caaaagtcag tataattatc ccggtttaca atgccggagg gtatttagtt
     1081 cgatgtcttg actcggttgt aagtcaaact ctgaaagata ttgagataat attggttctg
     1141 gataatccta ccgatggcag tgaccttgta gctgaacagt attcacagaa ggataaccga
     1201 attaagataa taaaaaacga gaataaccta cacatcggtt gttccagaaa caaaggctta
     1261 gaaattgcac agggtgaata tgttggtttt gccgatcatg acgattattg tcaacctgaa
     1321 atgtttgaaa tgctttacaa aaatgcagtc gaatttcagg cagatgtggt tgttagtgat
     1381 tattgtgacg aaaaacaaaa cggaattgat tattttggct ttcctacagg attaaccaat
     1441 gaagaattta aagtaaggtt gtttgatgca ttaatcagcg gccgacagtc acagccaaat
     1501 attcggacat ttgataatgt aaatgttatc tggaaccagc tttacaagca ggaatttttg
     1561 gttaactata atattaattt cagtgataac cgaattctga ctatggagga tgtacttttt
     1621 aatatcaaag ttcatcatta tgcaaaacag gttcgttata ttccggaggc tttttatcat
     1681 cacattacca acggacaaaa tgagtttcag aattactcct acagggcaat tgataaagtg
     1741 atgccgcatt tggtggaggt ttttgacttc ctggtgaaag aagatttata tcagcacttc
     1801 aaaaaatctt tcgccatttg tacattgaaa agattatata cttcgtatag aaatgaagtc
     1861 aggtttaagg gcatcctggc atcatttggt ttttttgaaa aagttagaag tcattcaaaa
     1921 ctccgggaaa tactgaacaa ttttaaggat aatacgttgt ggaagaatga attagctttg
     1981 acaaaacgtg catttttgat gatagtaagt aaataagcga aaatctatat tttttattgg
     2041 cgatatgaaa agcggtaatg tgttggtaac tgtcgtgatt ccggtattta atactgccga
     2101 atacctgaac gaagcaattg gttcgattct gcgccaaacc ttgcagaaac ttgagattgt
     2161 tgccattgac gattgctctt cagataattc actccaaata ctccgggaat tagctgctgc
     2221 tgatagccgg ataaaagtga ttgccatgcc gcaaaatcag ggtcaatcgg tgtgccgcaa
     2281 taagggtttg gaaatagcaa aaggtgaata catctatttt ttcgacagtg acgatatcct
     2341 ggaagaggat tgcttacaac tttgttatga aaaatgcacc gaattgcaaa ccgacctgtt
     2401 acttttcgac ggaaaatcgt ttaccgaaaa cggtatatcg ttggaattta atcctaaata
     2461 tgaccgtaca ggatttttta ccaaaagtgt ttcaaccggt ttggagacca ttgaaaccat
     2521 gtataaaaac aaggcttatt catgcagtgt ttgtttgatt tttatcagaa aatcctattt
     2581 ggatcaaaca ggattacgtt tttatccggg tgtgttttat gaggatgtcc ttttttcgat
     2641 gt
//
LOCUS       DAOPYB010000322         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_55485_length_2624_cov_6.588556, whole genome shotgun sequence.
ACCESSION   DAOPYB010000322 DAOPYB010000000
VERSION     DAOPYB010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55485_length_2624_cov_6.588556"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKH58_13605"
     CDS             <1..775
                     /locus_tag="PKH58_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNX90106.1"
                     /translation="EKIANRKIRENYPLQESRNTPIAEAQAMGAMALFGEKYGESVRV
                     IQFGNSIELCGGTHVQATGDIGMVRIVSESSIAAGIRRIEAITGQAVEKLLDLQQDTL
                     TTAKELFNNTPDLTKAIQKTLEENSELKKQVESNMHERILTLRDKLITDAKEENGVKV
                     IRYKGELPADAVKTLSFQIRNVINENLFVAITSLHEGKPSITVLLSDDLVAKGYNAVN
                     ITREAAKLIQGGGGGQPFFATAGGKNPEGLDKAMEMIKL"
     gene            806..1510
                     /locus_tag="PKH58_13610"
     CDS             806..1510
                     /locus_tag="PKH58_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNX90107.1"
                     /translation="MKTNFIKNMILLVAAFSVMHVGAQDWANLKRFQKENEMLSIKTK
                     AEKRVVFMGNSITEGWKNSHPEFFNKNPYICRGISGQTTPQMLLRFRQDVINLNANVV
                     VILAGTNDIAGNTGPSTPEMIMDNIRSMAELATANNIRVVLCSVLPALDYPWRKGLEP
                     NIKIPELNKMIKAYAEEKRFTYLDYFGAMANEKNGMITEYTTDGVHCTSMGYDVMEAM
                     VVPAINKALKTKPVKK"
     gene            complement(1653..1811)
                     /locus_tag="PKH58_13615"
     CDS             complement(1653..1811)
                     /locus_tag="PKH58_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90108.1"
                     /translation="MGRNYKFKDPEGVYFVSFAVVEWMIMQVKRIIGHCIDLRLVATR
                     YNRAAARG"
BASE COUNT          841 a          526 c          511 g          746 t
ORIGIN      
        1 tgaaaaaata gccaaccgta aaatccgcga gaactacccg ctgcaggaaa gccgtaacac
       61 accgatagca gaagctcagg caatgggtgc gatggcgctg tttggagaaa aatacggaga
      121 atctgttcgt gttattcagt ttggcaactc catcgaactc tgtggcggca ctcatgtgca
      181 ggctacgggt gatatcggta tggttcgcat cgtatccgaa agctccattg ctgcaggcat
      241 ccgccgcatc gaggcaatta ccggacaggc agtggaaaaa ttgctggatt tacagcagga
      301 tacgctgaca acagctaaag aattgttcaa caatacgccc gacctgacaa aggctataca
      361 gaaaaccctg gaggaaaaca gtgaactgaa aaaacaggtg gaaagcaata tgcatgagcg
      421 catcctgacg ctccgcgaca aacttataac cgatgcaaaa gaagaaaatg gcgtgaaagt
      481 tatccgttac aaaggcgaac ttccagctga tgctgtcaag actctttcat tccagatacg
      541 caacgtaata aatgaaaatc tttttgttgc tattaccagt ttacatgaag gtaaaccctc
      601 gattactgtt ttgctatcag atgatctggt tgcaaagggt tacaatgcgg tgaacattac
      661 ccgcgaagca gccaaactga ttcagggcgg cggcggtgga cagccattct ttgcaactgc
      721 cggtggcaaa aatcccgaag gactggacaa agcaatggaa atgattaagt tgtaatataa
      781 attgaatttc aaaactataa aaaccatgaa aaccaacttc attaaaaaca tgatcctgtt
      841 agttgctgca ttcagcgtaa tgcatgtcgg tgcgcaggat tgggctaatc tgaaacgctt
      901 tcagaaagag aatgaaatgc tttcaataaa gactaaggca gaaaaaagag tggtgtttat
      961 gggaaattcc attacagaag gctggaagaa ttcgcatccc gaatttttca ataaaaatcc
     1021 atacatctgt cgcggcatca gcggacaaac gacccctcaa atgctgttac ggtttcgtca
     1081 ggatgtaata aatctgaatg ccaacgtagt agtcattcta gccggaacaa acgacattgc
     1141 cggtaacacc ggtccctcga caccggaaat gatcatggac aatatcaggt caatggctga
     1201 actggcaacg gctaacaaca tcagagtggt actttgttcg gtactccctg ctctggatta
     1261 tccctggaga aaaggactgg aacccaatat aaaaatcccg gaactgaaca aaatgattaa
     1321 agcgtatgcc gaagaaaaaa gatttaccta tctggattat ttcggagcta tggccaatga
     1381 aaagaatggc atgatcactg aatatacaac tgatggcgta cattgcacat cgatgggata
     1441 tgatgtgatg gaagcgatgg ttgtgcctgc cattaataaa gcattgaaaa caaaaccggt
     1501 aaagaaataa ttcaataaga gttgaaagaa agagggtgct caaattgttt tgagcaccct
     1561 ctttgtatgt atttaagttg tgtgttccac actctttcgg gttagtctcc ttacttataa
     1621 tatactacag ggaataatgg gaaatcctgc tgttatcccc tcgctgccgc gcgattgtat
     1681 cgcgtggcaa caagcctcaa atcaatacaa tgtccaataa tccttttcac ctgcataatc
     1741 atccattcaa ccaccgcaaa gctaacaaaa tatactcctt ccggatcttt gaatttataa
     1801 tttctaccca ttataacttt tactcttttg aattactttc ttcttatctg acttgccacg
     1861 cgatacaatc gcgcggcagc aggggaaaaa gaatcaattt cttcccatgt ataaaatgta
     1921 tcactttcga gcctaccttt atcacaagac cttattcttc ttcctgaatc cgtcattatt
     1981 atccatttcc cttctctttt tcagtttttt caatccatca tttctattct catctttttt
     2041 cgaggtgaca acacttgctt aacgcattta aaattactta aaaatactgc ttatgagcct
     2101 gaaatatcat gcctctaagt tttggtggca taatactcta agatactgat tttctgatga
     2161 ttaaatatat aaattacttg ttattttatt gattaacagt tgaataaaaa aaaataacga
     2221 actgttgtaa gataattcat gtgtttttct tgcataaaga tgccatactt acaaactcat
     2281 ttctacaatt atataaattt aattatcttt gtaaattatt acatgtaggg ctgataaggt
     2341 tgtttttcaa cttcaaaata gcttttttgt ggcttatttg tactcagaat ttaagcagtt
     2401 ttcaatatgg agtattaaaa ttattacaaa taaaataatc gtaaaagaat gaagaaattt
     2461 ctgtttttgg taatattatt taccgtattt atctctaaaa caaatgccgt tccggcttat
     2521 ccttacccta tcaagtacac ccaacctgat ggttcggtga ttacggttca gctcaaagga
     2581 gatgagagag ttcactgggc tgaaagctca gatggatata cgct
//
LOCUS       DAOPYB010000323         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_56838_length_2583_cov_10.017405, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000323 DAOPYB010000000
VERSION     DAOPYB010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56838_length_2583_cov_10.017405"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1567
                     /locus_tag="PKH58_13620"
     CDS             344..1567
                     /locus_tag="PKH58_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX90109.1"
                     /translation="MENQKWFNTYIFIWAGQFVSMLTSYAVQFAIVIWLSLEYQSAEV
                     LAYAGIAGMLPQAIIGPLAGVYIDRTNRKRIMILSDTFIAVCAIVLIFILNKETVNLV
                     WIYIMLGLRSVGNAFHSPALQALAPLIVPKKELLRVAGINQMLHSICSIGGPALGTVA
                     IAILPISNVLYLDLIGALLAISSLMMVRIPQTIKGSKFSVQTISTEFIDGLHIVSKNR
                     GLSYLFLYAMAITFVIMPAAIMFPLLTTGHYGGGKWEMGLVEIVWGGGMLIGGAVLGL
                     FKVGMSKIVLVNSMYIILGLTFLLSGVFPKEWFIGFVLVTAIGGIGLSVFYACFTAIV
                     QITVKPEMLGRVFSLYYSLAVLPSIIGLLFTGVIAENIGINITFIISGCLAIVIGLLS
                     YGSEKLMQLGNNLKK"
     gene            1573..2460
                     /gene="mph"
                     /locus_tag="PKH58_13625"
     CDS             1573..2460
                     /gene="mph"
                     /locus_tag="PKH58_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E)/Mph(G) family macrolide
                     2'-phosphotransferase"
                     /protein_id="HNX90110.1"
                     /translation="MKNTDIQKLAERHGLMLSDKMKFNEMGIDFKVGFATDKDENQWL
                     LRIPRRPDLGKQIAKEKLILQLVCKHLTAQVPDWKIANEELVAYPLLDGKPALTYDAE
                     TYEVSWNMDKDSPNYIQSLAKILVQLHAIPKEEVVLNGLEVTTPESLRSEISERLRLV
                     KSELGISNDLENRYKKWLDNDTLWPNFTKFIHGDLYAGHILTYADGNVCGIIDWSTAR
                     ISDISQDFSGHLTVFGEESLKSLISEYEKQGGKIWNMIFEQAVERAAAAPLAYGYFAV
                     ETQDEVHINGAKVQLGVQR"
BASE COUNT          753 a          417 c          549 g          864 t
ORIGIN      
        1 taacgttacg cgtatatgat taggtgcgga ttgcgggttg cttccctgtc gaaaagcacc
       61 gaggctgatg cgggaaataa tgctgattat cagcaccgaa accgcactta attatatacg
      121 ctgttggcaa ctgggctttt ttacatccaa attcagacta tttatttctt caaatttaaa
      181 aaaatatttc ctacctttgt gcggttattt ccaataaatt atacaacaaa tggcaaagcc
      241 ttggataaaa taatagggta attctcaaaa tacccacatt tttttagata gctcttctca
      301 gtgctatcca tttattgttg tataatttaa tcaagtttat ttcatggaga accaaaaatg
      361 gtttaacacc tatatattta tatgggctgg gcaatttgtt tcaatgttga caagttatgc
      421 agtccaattt gctattgtca tatggcttag tctggagtat caatccgccg aggttttagc
      481 atatgcaggt atagctggaa tgttgccaca agctattatt ggcccgttag caggggtata
      541 tatagaccgt actaatcgaa aacgtatcat gattctttct gatactttca ttgctgtatg
      601 tgctattgtc ttgatattta tccttaacaa ggaaacggta aatcttgtat ggatttatat
      661 catgttaggt ttacgttcgg ttggcaatgc ctttcattca ccggctctac aagctttggc
      721 tccgctaatt gtgcctaaaa aagaattgct aagagttgct ggtattaatc aaatgttaca
      781 ttcgatttgt agtattggtg gtcctgcact tggtacagtg gcaattgcca tattgcctat
      841 ttcaaatgtt ttgtatcttg atttgattgg agcattactg gcaatttctt cgcttatgat
      901 ggtaagaata ccacagacaa taaaaggttc taaattttct gttcagacaa tatcaaccga
      961 atttatagat gggttacata tcgtatctaa aaatagaggt ttaagttacc tttttcttta
     1021 tgccatggct atcacctttg tcatcatgcc tgctgcaatt atgttcccac tactgacaac
     1081 aggacattat ggaggaggaa aatgggaaat gggccttgtt gagatagttt gggggggagg
     1141 catgctgatt ggtggagctg ttttaggctt gttcaaagtt gggatgtcaa agattgtatt
     1201 ggttaattca atgtatatca tattggggtt aacattcctt cttagcggag ttttcccaaa
     1261 agaatggttc attggatttg ttctggtaac agcaattgga ggtattggtt tatctgtttt
     1321 ttatgcatgt tttactgcta ttgtccagat aacagtaaaa cctgaaatgc tgggacgagt
     1381 cttttctctt tattacagtt tggcggtatt gccaagtata atcggtttat tgttcacagg
     1441 cgtgattgca gaaaatatag gtataaacat cacttttatc ataagtggat gtttggctat
     1501 cgttatcggg ttgctttcgt atggctctga gaaacttatg cagttaggta ataatttaaa
     1561 aaaataaaac aaatgaaaaa tacagacatt cagaaacttg cagaaagaca tggtttaatg
     1621 ctttcagaca agatgaaatt caacgaaatg ggaatagatt tcaaggttgg ttttgcaaca
     1681 gacaaagatg aaaatcaatg gctgttgcgt attcccagaa gacccgattt gggtaagcag
     1741 attgcaaagg aaaaactaat tctacagtta gtttgcaaac atctcactgc gcaagttcca
     1801 gattggaaaa ttgcgaacga agagctggta gcgtatccat tattggatgg aaaacccgct
     1861 ttgacttatg atgctgaaac ttatgaggtt tcttggaata tggataaaga tagtccaaac
     1921 tacattcagt cattggctaa aattttggtg caactccatg ctattccgaa agaagaagtt
     1981 gttctgaatg gattggaagt tactacacct gaaagtctga gaagcgagat ttccgaacga
     2041 ttacgcttgg taaaatccga acttggcata agtaatgatt tagaaaatcg ttacaaaaag
     2101 tggttggata atgatacact gtggccgaac ttcacaaaat tcattcatgg tgatttgtat
     2161 gcaggacata ttctaacata tgccgatggt aatgtatgtg gaattattga ctggtcaaca
     2221 gcccgaatca gtgatatttc ccaagatttt tcgggacatc tcactgtttt cggagaagaa
     2281 agtctcaagt ctttaatttc agagtatgaa aaacaaggtg gcaaaatatg gaatatgatt
     2341 tttgagcagg ccgttgagcg agctgcggct gcacccttgg cttatggtta ttttgctgtt
     2401 gaaacacagg atgaagtcca tataaacggt gctaaagtcc agttaggtgt tcagcgataa
     2461 tcgtgattaa ctttttatta tttagggctg gtcttatttt ttgaccagtc cttttattaa
     2521 tcgaaactta atcttttttc ctggggttga tgtgtgtcgc tcttagcctt gttgccaacg
     2581 gtt
//
LOCUS       DAOPYB010000324         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_57410_length_2567_cov_9.074045, whole genome shotgun sequence.
ACCESSION   DAOPYB010000324 DAOPYB010000000
VERSION     DAOPYB010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57410_length_2567_cov_9.074045"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..>2567)
                     /locus_tag="PKH58_13630"
     CDS             complement(256..>2567)
                     /locus_tag="PKH58_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HNX90111.1"
                     /translation="NSLLFDFQNDNQPIRENYSEIYSDSIGIKQVSVFKDFSKLIENV
                     ELSSIIHDIQNGRYKSEVEDIRAALSENNQYDADRLKKKLIGFTPSGTFNNGRKIKDI
                     TTYSGCIILDIDKLTPEQLTTAKETAVSIPYTYAAFVSPSGKGLKIIVTIDQGREQHA
                     AAMAQITNYYFKKLNLPIDPSGKDVSRLCFVSYDPDCYFNPKPEIFKANSSLEFKQKH
                     QLLKKVSIQVEDNPDQLFDDCVKFTERKSTYHIGNRNNFIHQLACNCNRKGIPFDTAL
                     SNIRQSYDLDDTELTKAVESAYNNNPNDFANSANFASSAVSKSDELSSKEAVMSNMPY
                     LPDDIFNSLPALLKDGCKVFTNKRERDVFLTGALSIFSGCMPNVVGVYMRKEVYANLY
                     TFTVAPAASGKGALTFSKELGQKYHEKLLQFSNDQRKIYNMKMEEYKKNSFSKNTEKE
                     SIEMPEEPPFQVLYIPGNNSSARVIQHLKESDGQGIFCETEADTIGNVFKQDWGSYSD
                     LLRKAFHHEPISYSRKKDKEFVEIKKPRLAVALAGTPGQVEGLIKSAEDGLFSRFLFY
                     VFRSEIEWMDASDTAGGYNLTTHFEALSIRVLQFVEFLQSTDKIHFSLNGNQWIKLNE
                     FGNSSISKLTTLISEEMASTSKRLGLILYRIAMIISALRYYDNGEVPIHMTCSDDDFD
                     IALKLINTYQEHAAFMFGQLPKSPGVADKTLKKFFDLLPDEFLRKDAVAIALTINIKE
                     RTADGYLSKLVSSKLLEQDKLGHYTKVKK"
BASE COUNT          693 a          508 c          489 g          877 t
ORIGIN      
        1 gtaaataatt ttttcatggg tattttttga tagattatac agaactcttt tatatgagtt
       61 ttgtcttata cttatatacg aatacaaaaa aaggcgcagc ctttcagcta cacccgacaa
      121 atataccaat aattttaata caaataattt ataattttaa taaaaggcag atagggtttt
      181 atttttgttc aatttcatcc ccctcacttc ctaatgttcc ggcagaaagc aagggggaaa
      241 tttatatctg ataaatcatt tttttacttt cgtataatgg cctaatttgt cctgttccag
      301 taatttgcta ctgaccaact tcgacaaata gccgtcggct gttcgttctt tgatgttgat
      361 agtaagagca atagcgactg catcctttct caggaactca tccggaagta aatcaaagaa
      421 ttttttcaat gttttatctg ccacacccgg tgatttaggc aattgcccaa acataaatgc
      481 tgcgtgttcc tgataagtgt tgattagttt taatgcaatg tcgaaatcat cgtcagagca
      541 tgtcatgtgg attggaactt caccgttgtc gtaatatcgt aatgcagaaa tgatcattgc
      601 tatccgataa agaattaacc ccagcctttt tgacgtactt gccatctctt cacttattaa
      661 agtggtcagt tttgaaatac tgctatttcc aaattcgttc aacttaatcc attggttgcc
      721 gttaagactg aaatggattt tgtctgtgct ttggagaaac tcaacaaact gtaatacccg
      781 gatggataat gcttcgaaat gagtagttag attatatcca ccagctgtgt cgcttgcatc
      841 catccattca atttcggaac gaaatacgta aaacaagaag cgactaaaaa gcccgtcctc
      901 tgctgatttg attaaccctt ccacttgtcc cggagtacct gccaatgcta cggctaaccg
      961 tggttttttg atttccacaa attccttatc tttctttcga ctgtaagaaa ttggttcgtg
     1021 gtggaatgct ttgcgtaata agtcggaata gctcccccaa tcctgcttaa atacatttcc
     1081 tattgtgtcg gcttcagttt cacaaaaaat accttgtccg tcgctttcct tcaaatgctg
     1141 gattactctc gcgctgctgt tgtttcccgg aatatataac acttggaatg gcggttcttc
     1201 gggcatttca atgctctcct tttcagtatt tttactaaag ctatttttct tgtactcctc
     1261 cattttcatg ttgtaaattt tgcgttggtc gttgctaaac tgcaaaagtt tctcatgata
     1321 tttctggcct aattctttgg aaaaggtcaa agcccctttt ccgcttgcag caggagccac
     1381 agtaaaagtg tacagatttg catagacctc tttacgcata taaactccca ctacgttggg
     1441 catacacccg ctaaaaatac ttaaagctcc tgttaagaac acatcacgct ccctcttgtt
     1501 agtgaacact ttacaaccat cctttaggag agcaggtaac gaattaaata tatcgtccgg
     1561 aagataaggc atattgctca ttactgcttc ttttgaggat aattcgtcag atttggaaac
     1621 agcagagctt gcaaagtttg cagagtttgc aaagtcgttc ggattgttgt tgtatgcgct
     1681 ttcgacagct ttggtcaatt ctgtatcgtc caaatcatac gactgcctga tattggatag
     1741 tgctgtgtcg aatggaatac cttttcgatt gcaattgcaa gctaactgat ggataaagtt
     1801 gttgcggttg ccgatgtgat aggtagattt acgttcagta aattttacgc aatcgtcaaa
     1861 cagctggtcg ggattatctt cgacctgaat gctaactttt ttcagtaatt gatgcttctg
     1921 cttaaactct aatgacgaat ttgccttgaa gatttccggt ttcggattga agtagcaatc
     1981 cgggtcgtaa gacacgaaac aaagtctgga aacatcttta cctgatggat caataggcag
     2041 atttagtttc ttgaaataat agttggtgat ttgtgccatc gcagcagcat gttgttctct
     2101 accctggtct attgttacaa tgatttttaa tcccttaccg cttgggctaa caaaagcagc
     2161 ataggtatat ggaattgata ctgctgtttc tttggctgtt gtaagttgtt cgggtgtgag
     2221 cttatcgata tccagaatga tgcaaccact gtaagtggtg atatctttga ttttccgtcc
     2281 gttgttgaaa gttccggatg gtgtaaatcc gatcagtttc tttttcaagc ggtcggcgtc
     2341 atattgattg ttttcactta atgccgctct gatatcctct acttctgatt tgtaccttcc
     2401 gttttgaatg tcgtggatga tgcttgataa ttctacattc tcaatcagct tagagaagtc
     2461 cttaaacaca gaaacttgct taattccaat actatcggaa tagatttcgc tataattctc
     2521 ccggataggt tgattatcgt tttggaaatc aaacaataag gagttat
//
LOCUS       DAOPYB010000325         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_57806_length_2555_cov_22.665600, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000325 DAOPYB010000000
VERSION     DAOPYB010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57806_length_2555_cov_22.665600"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..>1201)
                     /locus_tag="PKH58_13635"
     CDS             complement(59..>1201)
                     /locus_tag="PKH58_13635"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNX90112.1"
                     /translation="YFEFGKEKENLAGKLVKRFGGTKILIHYGSGSVVRSGLLQRVKT
                     SLDKENIEYVELGGVLPNPRSGLVYEGIELCRKEKVDFILAVGGGSAIDSAKAIAIGV
                     PYEGDFWDFYQGKRIEKALPVATVLTLSAAGSEASLSSVITHENGMVKRGTGSDKIRP
                     VFSILNPALTQTLPAYQTACGATDMFAHVLERYFTNTRNVEITDRLCEGIMLTIINET
                     PKALANPDDYDARANIMWAGMVAHNDICGVGREQDWGTHQLEHELSALYDVAHGAGLA
                     VMFPAWLKYNLKHDVMRYAQFATRVWGCQMNFENPEITAHEGIVRFETFLKSIGMPIR
                     FHELGARKEDIPTLISMLKLNGRKVGSFVQLAEKDIEAIYKLAAED"
     assembly_gap    1202..1301
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1302..>2555)
                     /locus_tag="PKH58_13640"
     CDS             complement(<1302..>2555)
                     /locus_tag="PKH58_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNX90113.1"
                     /translation="HIGSGVKCTDFIICSGVSISDSTLVSTCFIGQGCIMDKHYSALD
                     SLFFSNCQGMHGEATAIFAGPYTVSHHKSSLLIAGMFSFLNAGSGSNQSNHMYKLGPI
                     HQGIVERGSKTTSDSYILWPAKIGTFTLVMGRHTSHSDTTDLPFSYLIENGTESYLIP
                     GVNLRSVGTVRDAQKWPKRDNRTDDLKLDPINFNLLSPYTIQKMYKGIDVLENLKKIS
                     GETTEVFTYQNCKIKHSSLEKGIQFYRTAIYKFLGNSLISRIQKENPTTFKELQQKLR
                     SETNAGLGDWIDLSGLIVPKGEVEKMLTDIENNTLTLTGTRGRLEEMHNEYYNYEWTW
                     AQHKLEEYWGKPITTIGKEDLIAFIELWKESVVRLDNMLYEDAKKEFNLSSKIGFGVD
                     GDEEQKHKDFENVRGTFEKNPFVNEV"
BASE COUNT          581 a          572 c          580 g          722 t
ORIGIN      
        1 gcagacaaaa aaaggaaact aaataatctt taatttcctt tttaatagca gttgtgtttt
       61 aatcttcggc agccagtttg tagatggctt ctatatcttt ttcggcaagt tgtacaaaac
      121 tcccgacttt tcggccattc aatttgagca tactgattag tgttggaata tcttcttttc
      181 tggcaccgag ttcgtggaac cgtattggca tcccgattga tttcaggaag gtttcgaacc
      241 tgacgattcc ttcgtgtgct gtgatttcag gattctcgaa attcatctga cagccccaca
      301 cgcgagtggc aaactgcgcg taacgcatca cgtcgtgttt caggttgtat ttcaaccacg
      361 caggaaacat tacagccaat ccggcaccgt gtgccacatc ataaagtgcc gaaagttcat
      421 gttccagctg gtgtgttccc caatcctgtt cgcgtcctac gccacaaata tcattatgag
      481 ccaccatacc tgcccacatg atgttggcac gggcatcata atcatcggga ttagccagtg
      541 ctttgggtgt ttcgttaatg atagtgagca tgattccttc acagagccgg tcggtaattt
      601 ccacgttgcg ggtgttggtg aagtatcgtt cgagcacatg agcaaacatg tcggttgcac
      661 cacaagcggt ttgatatgca ggtaaagttt gggtcaatgc gggattaagg atggagaaaa
      721 ccggacgaat tttgtcactt cctgtgccac gtttcaccat tccgttttcg tgagtgatta
      781 ctgatgagag cgaagcttca cttcccgcag ccgaaagggt taatacagta gcaaccggta
      841 atgccttttc aattcgtttt ccctgataaa aatcccagaa atcaccttcg taaggtacac
      901 cgatggcaat ggctttggct gagtcgatgg cactgccgcc gcctaccgcc agaatgaaat
      961 ccactttttc cttccggcac aattcaatgc cttcgtacac caacccactg cggggattgg
     1021 gtaacacgcc gccaagttca acgtactcaa tattttcttt gtcgagggaa gttttcaccc
     1081 gttgcagcaa tccgctgcga actacagaac cacttccgta atgtatcagt attttcgtgc
     1141 cgccaaaacg tttcaccagt ttaccggcaa ggttttcttt ctcttttccg aactcaaaat
     1201 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntacttcatt gacaaaggga
     1321 tttttctcga aggtgccacg tacgttttcg aagtctttgt gcttttgctc ttcgtcgcca
     1381 tccactccaa acccgatttt ggagctcagg ttgaattcct ttttggcatc ttcgtagagc
     1441 atgttgtcga gacgaactac cgattctttc cacagttcga taaaagcaat aagatcttct
     1501 tttccgattg tcgttatggg ttttccccaa tattcctcca acttatgttg tgcccaggtc
     1561 cattcgtagt tgtagtattc gttgtgcatc tcttccaggc gacctcgggt accggtaagt
     1621 gtgagtgtat tgttttcgat atccgtaagc attttttcta cttccccctt gggtactatc
     1681 agcccggaga gatcgatcca gtcacccaaa ccggcattgg tttccgaacg aagcttttgc
     1741 tgtaattctt tgaatgttgt cggattttcc ttctgtatcc ggctgatgag tgaattgccc
     1801 aaaaacttgt agatagcggt gcgataaaac tgtatccctt tttcgaggga agaatgtttt
     1861 attttgcagt tctgataggt gaacacctcc gtggtttcgc ccgaaatctt tttcagattt
     1921 tcgagtacat caatgccttt atacattttc tgtatggtat aggggctgag caggttgaag
     1981 ttgatcgggt ccagtttcaa atcatccgtg cggttatcac gtttcggcca tttctgggca
     2041 tcgcgcaccg tacctacgct gcgcaggttc acaccgggaa tgaggtaact ctctgtgcca
     2101 ttttcgatga ggtaggagaa gggcaggtcg gtggtatcgg agtgtgaggt atgtcgcccc
     2161 atgaccagtg tgaaagtacc aatcttggca ggccagagga tgtatgagtc gctggtggtt
     2221 ttcgacccgc gttccacaat gccctgatga attggtccga gcttgtacat gtgattgctt
     2281 tggttagagc cgctgccggc attgaggaag gagaacattc cggcaatgag caggctcgac
     2341 ttgtggtgtg atacggtgta aggtcctgca aaaatggcgg tagcttcacc gtgcatgccc
     2401 tgacagttgg agaaaaacaa cgaatcgaga gcagaataat gtttatccat tatgcaaccc
     2461 tgaccgatga agcaggtgga taccagcgtg gagtcgctga tggatactcc ggagcaaata
     2521 atgaaatcgg tgcatttaac acccgacccg atatg
//
LOCUS       DAOPYB010000326         2537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_58426_length_2537_cov_9.510475, whole genome shotgun sequence.
ACCESSION   DAOPYB010000326 DAOPYB010000000
VERSION     DAOPYB010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58426_length_2537_cov_9.510475"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..842)
                     /gene="istB"
                     /locus_tag="PKH58_13645"
     CDS             complement(87..842)
                     /gene="istB"
                     /locus_tag="PKH58_13645"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNX90114.1"
                     /translation="MAELTMVASKLRHLSLSEAAAGLESIVAEAALAENTPLEVLNKL
                     LQMEIDGRNAKGRAKRLKAACFPYVEDLESFNFQRNGFKGINKTHIRQLAELAWLEKA
                     YNIMFLGPTGLGKTRLSIGLGLKAVEAGYNVSFVTLDELMRLLKTAEISTRAARRVKT
                     IKNSDLVILDEVGFLPISKQDANKLYEFVNVLYLKTSVILTSNKDFEEWSEFIGDSII
                     TAAILDRLAHQCEIFSLDGPSYRLENRKTIFGN"
     gene            complement(830..2356)
                     /locus_tag="PKH58_13650"
     CDS             complement(830..2356)
                     /locus_tag="PKH58_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HNX90115.1"
                     /translation="MKSQGYSQRKVARTLEIHRTTVKKYWYMTPEEYNDSILEPAKKS
                     RLEQHKELIVSWLRTYPEVTAAQIYDWLQEKYGLQLAENSVRRYIRILRIDYDIKPVA
                     DDREYEAIQDPPMGLQMQVDIGSISVENIRTRRYQKLYCIGFVLSHSRYKYGVWFPSP
                     PEAQDMINAIRSCFEWMGGKPRELVFDQDRLIAVNENYGDIIYTKEFEAFRQSEKLDI
                     YLCRKGDPPSKGRIEAVVKFFKYNFARYRQYTEQWVWNEEFEQWLHRTGNAKKHSITK
                     KIPAEVFAIEQGYLTPVSYEINKSDDNIVIRKIRKDNTVMYEGNRYTVPTGSFGLHDD
                     VQLEIDDDELLIYAGFCDILLAKHKICFEKGKLIQNNDHLRNKEIKIAEMKQSLKAKF
                     ANPEAAGLFLNGIHNRKKRYVRDQFLLIEKAIGEYDPETVASALDYCLINELYSAVDF
                     KDALNHFSQGVKKVPLSTNPVTIKSTSTTTAISNVEVAKRDLQEMIQSLKGGNDPWLN
                     "
BASE COUNT          630 a          569 c          462 g          876 t
ORIGIN      
        1 tgtgaatgtc aatataaaat gtaccaaaaa cgctaatatt ttttgtacca ctttttagtc
       61 aaagtagcta aaactgctac cagggattag ttcccaaaaa tggtttttcg attttctaat
      121 ctgtaacttg gaccatccaa tgaaaatatc tcacattgat gggccaggcg gtcgaggatt
      181 gctgctgtaa taatgctgtc cccgataaat tcgctccatt cctcaaaatc tttattactt
      241 gtcaatatga ctgatgtctt taaatacagc acattcacaa attcatataa cttgttagcg
      301 tcttgctttg agatgggcaa aaagcccacc tcatcaagga taaccagatc actgttctta
      361 atggttttta ctcttctggc agctcgggtg ctaatctccg ctgtcttaag aagtcgcatc
      421 agttcatcca aagtaacaaa ggatacatta tatccggctt ccaccgcctt taatcctaat
      481 ccgatggaca acctggtttt acccaagccc gtaggcccta aaaacataat gttatatgct
      541 ttctccagcc atgccaactc tgctaactga cggatatgcg ttttgttgat tcctttaaaa
      601 ccattgcgct gaaaattaaa gctttctaaa tcctccacat aagggaaaca ggcagctttc
      661 agccttttag cccttccttt ggcattcctg ccgtctatct ccatttgcaa tagcttattc
      721 aatacttcca gtggagtatt ttccgccaat gctgcttctg caacaataga ttcaagtcca
      781 gcggcagctt cagaaaggct cagatgtctt aactttgatg caaccattgt caattcagcc
      841 atggatcatt acctcctttc agactttgga tcatttcctg taagtctctc ttggctactt
      901 caacattgga tatagccgtt gttgtggatg tactttttat cgttacagga ttagttgaaa
      961 gaggtacttt cttaacgccc tggctaaaat ggttaagtgc atccttgaaa tccactgcac
     1021 tgtaaagctc gttaattagg cagtaatcca atgctgatgc aacggtttcc gggtcgtatt
     1081 cacctattgc cttttctatt aacaggaatt gatcccggac atatcttttc tttctattat
     1141 gtatcccatt aagaaataat ccggctgctt cagggtttgc aaactttgct ttaagggatt
     1201 gtttcatctc ggctatcttt atctctttat tcctgagatg atcgttgttt tgtatcagtt
     1261 tccctttttc aaagcatatt ttgtgtttgg ccagcagtat gtcgcaaaaa cctgcatata
     1321 tgagcagctc atcatcatct atctccaact ggacatcatc atgtaatcca aagcttccgg
     1381 tggggactgt atatctgttt ccctcgtaca tgacggtgtt gtcttttcta atttttcgga
     1441 ttactatatt atcatcagat ttatttattt cgtatgatac cggagtgagg tagccctgtt
     1501 cgatggcaaa tacctctgcc ggtatttttt ttgtaatgga gtgtttcttg gcgtttccgg
     1561 tacggtgaag ccattgttcg aattcttcat tccacaccca ttgctcggta tattgccggt
     1621 atctggcaaa gttgtattta aagaatttaa caactgcttc tatccggcct ttacttggag
     1681 gatctccctt tctgcaaagg taaatatcca gtttctcact ttgccgaaag gcctcaaatt
     1741 ctttggtgta tatgatatcc ccatagttct cattaaccgc aattagccgg tcttggtcaa
     1801 ataccagttc tctgggcttg cctcccatcc attcaaaaca ggaacgaatg gcatttatca
     1861 tatcctgtgc ttcaggcggg gatggaaacc atactccgta tttatatctg gaatgtgaga
     1921 gtacaaagcc tatgcagtaa agcttctgat atcgtcttgt ccgaatattc tctactgata
     1981 tgctgccaat atccacctgc atttgcagcc ccataggtgg gtcttgaatt gcttcatact
     2041 ctcgatcgtc agcaaccggc tttatatcat agtcaatacg taaaatcctg atatatcttc
     2101 taacactatt ttccgcaagc tgcaggccgt atttttcctg aagccagtca tatatctgtg
     2161 ctgcggtaac ttccgggtaa gtccttagcc aggacacaat cagttctttg tgctgctcaa
     2221 gacgggattt cttggccggt tcaagtattg agtcattata ttcttccggg gtcatgtacc
     2281 aatatttctt gactgtagtc cgatggattt ctagagttcg cgctaccttt cgttgtgaat
     2341 atccttggct tttcattgct tgaattttgc tgtacatttg ccacctctta gtcattttta
     2401 acctcccatg ctatttcttt ctggtaatta aaccactcaa gataataaca tgaaggttgg
     2461 gctgtttcca ttactgactt tttttagtgg cacacttttt attagcgttt ttggtacact
     2521 ttattttagc gtttaca
//
LOCUS       DAOPYB010000327         2525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_58886_length_2525_cov_9.763563, whole genome shotgun sequence.
ACCESSION   DAOPYB010000327 DAOPYB010000000
VERSION     DAOPYB010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58886_length_2525_cov_9.763563"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1529
                     /gene="mnmE"
                     /locus_tag="PKH58_13655"
     CDS             138..1529
                     /gene="mnmE"
                     /locus_tag="PKH58_13655"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNX90116.1"
                     /translation="MEKTICAISTAPGVGGIAVIRVSGVDAISICNAVFRPLNTDEPL
                     INQPAYTVKYGTVVNEKSEPVDEVLATVFRAPHSFTGEDVVEISCHGSRYIQQQILQL
                     LIHQGCSMAKAGEFTMRAFQNGKMDLSQAEAVADLIASTTSASHRMALNQMRGGFSSE
                     LKELRARLLDFVSLVELELDFSEEDVEFADRSKLTELAVEIEEKINALAGSFKVGNVL
                     KSGIPVALVGETNVGKSTLLNLLVNEEKAIVSDIHGTTRDTIEDMVDIDGISFRFIDT
                     AGIRKTKDTIENLGIERTYQKIEQSDIVLWIVDCTQVSEHIEWLTEKIARRAAGKKII
                     LVFNKIDKLVDDELEVIEQLFSRFEAERVYISARKNINTDQLRQALINASQIKEIQTG
                     DVIVNNMRHYEALMNALSAIQRVSEGISSGIPADFLSRDIRECMYHLGEITGQISSDE
                     ILGNIFGKFCIGK"
     gene            1846..>2525
                     /locus_tag="PKH58_13660"
     CDS             1846..>2525
                     /locus_tag="PKH58_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX90117.1"
                     /translation="MFDNYPKKRTALPENFQRIYNEHYKNNREGKTSASSVSQRMERW
                     LHKKVAGDVKGINNKSTLEIGAGTLNQLEYEQTRPYDIVEPFTELYRNSPHLSRINHI
                     YSDISEIGRDKTYDRITSVATFEHLTDLPGVIARTCLLLNKNGTLRVSIPNEGTFLWK
                     LGWKLTTALEFRIKYGLDYEQLMQHEHVNTAQEIEQALKYFYSNVSCSCFGLNKKVAF
                     YLYYECSLP"
BASE COUNT          769 a          571 c          577 g          608 t
ORIGIN      
        1 gccatcgggg cgaacgcggt agtcaaccgc gacatacccg acaatgttac cgcgggaggt
       61 attcctgcca ctgttatttc aaataaatca tcactgtcgg caggcttatt ccccgacaac
      121 cttacaaaga actgaaaatg gaaaaaacaa tttgcgccat ctccactgct cccggtgtcg
      181 gaggtatagc tgttatccgg gtatccggag tggatgccat cagtatttgc aatgctgttt
      241 ttcggccact gaatacggat gaaccactga tcaatcagcc tgcatatacg gttaaatacg
      301 gtactgttgt caacgaaaaa tccgaaccgg tagatgaggt acttgctacc gtattccggg
      361 cgccgcattc atttaccggc gaagatgtgg ttgagatttc ctgtcacggt tcgaggtata
      421 tacagcagca gatattacaa ctgcttattc atcagggctg ttccatggct aaagccggag
      481 agttcaccat gcgggctttt cagaacggga agatggatct ttcgcaggcc gaagccgtgg
      541 ccgatctgat agcttccacc acatcggcat cgcatcgaat ggccctcaac cagatgcggg
      601 gaggattttc atcggaactg aaggaactcc gcgcacgcct gctcgacttt gtctcactgg
      661 tggaactgga acttgatttc agtgaagagg atgtggaatt tgccgaccgc agcaagctta
      721 ctgaacttgc cgttgaaata gaagaaaaaa taaacgcgct tgccggctca ttcaaagtag
      781 gaaatgtact gaagtcaggt atacccgtag ccctggttgg tgagaccaat gtagggaaat
      841 ccacgctact gaatttactg gtcaacgaag aaaaagccat cgtatcggac atacacggca
      901 caacccgcga caccatcgag gatatggtgg atatcgacgg catatccttc cgcttcattg
      961 acactgccgg tatccgaaaa accaaagata ccattgaaaa cctgggtatc gaacgtacct
     1021 atcagaaaat agagcaatcc gacattgtac tctggattgt ggactgcaca caggtgagcg
     1081 aacacatcga atggctgacc gagaagatag cacggcgtgc agccggaaag aaaatcatac
     1141 tcgtattcaa taaaatagat aaacttgttg atgatgaact cgaagtaatt gagcagctct
     1201 tctcacgttt cgaagcggaa cgggtgtaca tttcggcacg gaaaaacatc aataccgatc
     1261 aactccggca ggcactgatc aacgcgtcgc agataaaaga aatacaaacc ggtgatgtga
     1321 tagtgaacaa catgcggcac tatgaagccc tgatgaatgc cctgagtgct attcagcgtg
     1381 tgtcggaggg catcagctcc ggtatcccag cagactttct gagccgcgac atcagggaat
     1441 gcatgtatca tctgggtgaa atcaccggac agatatcatc ggatgaaata ctcgggaata
     1501 tcttcgggaa attctgcatc ggaaagtaac agtatgctta cacacgcaaa cattacagct
     1561 caaaccagtt taaaagtacc gacagatgta agtctttgat gatttaataa aacaacctga
     1621 gttcgacata agaaatagtc cgttaggact taaatatcaa tagaaagcgt gtttttgtat
     1681 tggttcattt cccgtaggga attgatatat ttatccccta acggagaatt taatttattt
     1741 agtaaccttt ttctatagat attaattccc tacgggaata cagacaattg tacctgaatt
     1801 ggttaaatgt gtttttacat cgaactcacg ttaaaattta tccccatgtt tgacaattac
     1861 cccaaaaaaa gaacagcatt acctgagaat tttcagcgaa tatataacga gcactataaa
     1921 aacaaccggg agggtaagac ttcggcatca tccgtatcgc aaagaatgga aaggtggtta
     1981 cacaaaaagg ttgccgggga tgtaaaaggg ataaacaaca agtcgaccct tgaaattggc
     2041 gcaggcacac tgaaccaact cgaatacgag caaacgagac cctatgatat tgtggaaccg
     2101 ttcaccgaac tttacaggaa ctcaccgcac ctgagcagga taaaccacat ttacagcgac
     2161 atcagtgaaa taggtcgtga taagacttac gacaggataa catccgttgc caccttcgaa
     2221 cacctgacgg atctgcccgg agttattgcc agaacctgcc tgctgttaaa taaaaacggc
     2281 accttacgcg tttctatacc caacgaagga acatttctct ggaaactggg gtggaagctg
     2341 actaccgccc tggagttcag gattaaatac ggcctggatt atgagcagtt aatgcagcat
     2401 gaacatgtca atacggcaca agaaattgaa caggccctga agtattttta ttccaatgtc
     2461 agttgctcct gctttggact aaacaaaaag gttgcgtttt atctttacta cgaatgttcc
     2521 ctgcc
//
LOCUS       DAOPYB010000328         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_59347_length_2512_cov_9.402930, whole genome shotgun sequence.
ACCESSION   DAOPYB010000328 DAOPYB010000000
VERSION     DAOPYB010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59347_length_2512_cov_9.402930"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..2344
                     /locus_tag="PKH58_13665"
     CDS             311..2344
                     /locus_tag="PKH58_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90118.1"
                     /translation="MKAIFISFIILSTSILVQAQWIRTNGPPLGEINALLCDSSYMYA
                     ALSGRGIYLSTDNGNSWAKKNNGLTTPFISDIVSLGTNLFAGTTGDGVFLSTDNGDNW
                     TPVNNGLTYPFIYSLTVNESELWAGTAGGAFLSTDNGSSWTKMNNGMPYAVNVGSIVI
                     NGNNIFAGTDFGVYLSTDNGNLWSPVNNGLLSPYISSLSLKGDSIFAITHECGIFLST
                     NNGNSWTSFNEGLDLTLVQTLIANNTGLFAGTDNGVYLYMNDVNSWIPVNSGLKNVKV
                     ITLAVDGLNLIAGTTLYGMFKSIDNGVSWNSLNNNLPHVGVFTMTNFGTMLITGNLWN
                     GICTTEDNGETWSIAIDGLTNIVTQSLITEDNIIFAGTDNGVFRSNDSGYSWHPTYNG
                     ITNLDILALVSVDSILYAGTYGGGVFKSTDNGISWTATNNGLTDLDVRSFAVIGTKLF
                     AGTLGGGIFLSSNYGDDWIEINNGLTGMRVASMTVTDTTLFAGTFEDGIFRTDDYGDT
                     WLKIDSGIEGMRVESLISFGNDIYAGTYENGVRLSNDNGNTWIEANDGLTAMHVLSFA
                     IIGTTLYAGTNDGVFINFSLLTNRDLNAKISQGIRIYPNPGRVYLVIQSEPQVNGAIF
                     RMFDMQGKPVIELRLTSTQLRLDTNDLATGTYSWQLLNNNKVIESGKWLKGNR"
BASE COUNT          771 a          428 c          525 g          788 t
ORIGIN      
        1 cctgcaagac attcctcgaa atgaaagacc cttcgtcaac cgatgaagag catcggcagg
       61 cgattattga cagggttgcg cagctgaaaa acagtgctgg cgaatgtttt tcctgattta
      121 taactgagtt gttgcatcag atttttttga tttactgcga actaatgatt gatgcagtaa
      181 taattggttg taaatcatac gaattgtaac tatatttcgt gtaagattca tttgatcaac
      241 aatgaaacta attattgaac tgtcaaagaa ataaattttc acataaacct cattgaaaca
      301 aaatgctata atgaaagcta tattcatatc attcataatt ctttctactt cgatattagt
      361 gcaagcccaa tggataagga ctaacggccc tcccttaggc gaaataaatg cattgctttg
      421 tgatagttca tatatgtatg cagctctttc tggacgaggt atttatttat cgactgacaa
      481 tggaaactcc tgggctaaaa agaataatgg attaacaact ccctttatca gtgacatagt
      541 ttcattaggt actaatcttt tcgccgggac tactggtgat ggggtttttt tatctactga
      601 taacggagat aattggactc ctgtcaacaa tgggctaacc tatccattca tatattcatt
      661 gacagtaaac gagtcagaac tatgggcagg aaccgcaggt ggagcttttt tatcgactga
      721 taatgggagt tcatggacta agatgaacaa tggaatgcct tacgcagtaa atgtaggatc
      781 tattgttatt aacggtaaca atatttttgc cggaacagat tttggcgtgt atttatcaac
      841 tgataatggc aacttgtggt ctcccgtaaa taatggatta ttaagcccct atatttcatc
      901 actgtcatta aaaggggact ctatatttgc catcactcat gaatgtggaa tctttttatc
      961 taccaataat ggtaattcat ggacatcatt caacgaaggt ttagacttaa ccttggtcca
     1021 aacattaatt gctaataata ccggtttatt tgcaggtacc gataatggtg tatatcttta
     1081 tatgaatgat gtaaatagtt ggatacctgt taatagcggg ttgaaaaatg taaaggtgat
     1141 tacattggct gttgatggtt taaatcttat tgcaggtaca acattgtatg gtatgtttaa
     1201 atctatagat aatggagtat catggaacag cttaaataat aatctccctc atgtaggtgt
     1261 ttttaccatg acaaattttg gtactatgct tattaccgga aatctttgga atggcatttg
     1321 cacaactgaa gataacgggg aaacatggtc aatagcaatt gatggattga caaatattgt
     1381 aacccagtca ttgattactg aagataatat aattttcgcg ggaaccgaca atggtgtttt
     1441 tcgatcaaat gactccggat atagctggca tccaacttac aatggtataa ctaacttgga
     1501 tatattagcg cttgtttctg ttgattccat cttatatgca gggacgtacg gcggaggggt
     1561 tttcaaatcg acagataatg gaatttcatg gacggcaaca aacaatggct taactgatct
     1621 tgacgtaagg tcatttgctg ttatagggac caagcttttt gcaggaactc tcggtggcgg
     1681 aatattttta tcctcaaatt acggtgatga ttggatcgaa ataaataacg gattgaccgg
     1741 aatgagagtg gcttcgatga cagtgaccga taccacattg tttgcgggta catttgaaga
     1801 tggtattttc aggactgacg attacggaga tacatggctt aaaattgatt ccggcattga
     1861 aggaatgaga gttgaatctt tgatttcttt tggaaatgac atttatgcag gaacctatga
     1921 gaatggagtt aggttgtcca atgataatgg aaacacatgg attgaagcaa atgatggctt
     1981 gacagcgatg catgtattat cattcgcaat tatcggaaca actttatatg ccggaacaaa
     2041 tgatggcgtc tttattaatt tctccttact cacaaatcgt gatttgaatg ccaaaatttc
     2101 tcagggaatc aggatctatc ccaatcccgg ccgtgtgtat ctggtaattc aatcagaacc
     2161 gcaagtaaat ggggcgattt tcagaatgtt cgatatgcag ggcaaacctg tgatcgaatt
     2221 gcggttgaca tctactcagc taaggcttga tacaaatgac cttgccaccg gcacctattc
     2281 atggcaactc cttaataaca ataaggtaat tgaaagcggg aaatggttga aagggaacag
     2341 ataaattatt cgatatctgt taaatatcaa gctattagtt cttcatgatc aacattagca
     2401 gaatttcccc cgcaccatcc tttaacatct tcaatcctca aagcgggaat ttcaagcttc
     2461 agctttttgc gcaggccgct caggtcgttg agtaatttgt tgggattggc ag
//
LOCUS       DAOPYB010000329         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_62069_length_2440_cov_15.142558, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000329 DAOPYB010000000
VERSION     DAOPYB010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62069_length_2440_cov_15.142558"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..577
                     /locus_tag="PKH58_13670"
     CDS             83..577
                     /locus_tag="PKH58_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HNX90119.1"
                     /translation="MQNFAAIDFETANQYPTSVCSVGVVVVRDGVITDKFYSLIRPEP
                     EFYSYWNTRVHGIRMEDTLHAPVFPQVWQQIEPLIEGLPLIAHNKGFDERCLKEVFRM
                     YQLDYPDYEFHCTLQRSKKQLKGLLPNFQLHTVSGYCGYELVNHHHALADAEACAWIA
                     MQVF"
     gene            complement(744..2051)
                     /locus_tag="PKH58_13675"
     CDS             complement(744..2051)
                     /locus_tag="PKH58_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90120.1"
                     /translation="MKRFSLYFSLLILVISQLSAQNDYNGFDLSKYYTPDIKRNSLEF
                     QFNMNGSATNYRDTTTASSFNLQGTQLNFSNLLNTRKKISTFQTGIDVGLGYSKFTDK
                     NFSFSNSDYVRWFNKLYYSGKHFFIYNATVNYYGNYQKITKTQIPDITTYKTSEVNVI
                     PEIGWGIGRIERVEDARQAIYILEEFKRNNVLKREMDKEEIFEFAQLISTVKNKRFLD
                     SRLHRIDEISTLDNYLKNKGVLNNVDAPYFTSLYDMWDYGALFNRSSGLELSFKVSPT
                     YYCKAYDETNSDFQTKYFGVISSASFNYSKPVKLNWQHDFSAKANFGIYDIIPGYNDA
                     ELYYIPSAELSYQLNYYPTTRSSLYAQTRYNFNSQQSSDVTSKYLSQQFLLGAGANYY
                     VSQNVRLSGYAGINSKMNKVNDFFYGENNSYIYFNFSLKYMFF"
     gene            complement(2153..>2440)
                     /locus_tag="PKH58_13680"
     CDS             complement(2153..>2440)
                     /locus_tag="PKH58_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNX90121.1"
                     /translation="GVRVFIYKKGFLHSKAIVLDDFVSIIGSTNMDDRSFDQNFEANA
                     FIYDTKTALQLKELFMNDLNKCEELTYDAWNNRKRRQKLKESFARLFSPLM"
BASE COUNT          765 a          441 c          452 g          782 t
ORIGIN      
        1 tcttgttacc taatcatatc aataaaaaga ttttttgtag tagcaaaatc tattcttact
       61 tttgtgaaaa caaaattctc ctatgcaaaa ctttgccgcc atagatttcg aaacagccaa
      121 tcagtatccc accagtgtat gcagtgtggg agtggtggtg gtgcgtgatg gtgtgataac
      181 cgataagttt tacagcctga tacgacctga accggagttt tacagttatt ggaatacccg
      241 agtgcacggc atccggatgg aggacacgct gcatgctccg gttttcccac aagtttggca
      301 gcaaatagaa ccgcttatcg aaggattacc gttgatagct cacaataaag gatttgatga
      361 acgctgcctg aaggaagttt tcagaatgta tcagctggat tatcccgatt atgagtttca
      421 ctgcactctg caaaggtcca agaagcaact gaaaggactt ttacccaact ttcagctgca
      481 cacagtatca ggctactgcg gttatgagtt ggtaaaccat caccatgcac tggccgatgc
      541 tgaagcgtgt gcctggatag caatgcaggt attttaatcc ctacaataaa taattcgtag
      601 atgctatccg tatagaatat gaggatataa attacagatt tcaggtaaaa tataattact
      661 cgtattcaat tcaaaaatta caggataaaa caaaaccctc cgttatgaca gagggttttt
      721 taattaatat cggtttattt attttagaaa aacatatatt tcaacgaaaa attgaaatat
      781 atatatgagt tattttcacc atagaaaaaa tcattcactt tattcatttt tgaattgata
      841 ccggcgtatc ccgacagacg tacgttttgt gaaacatagt aattggcacc tgcgccaagt
      901 aagaattgct gactgagata tttagaggtt acatcacttg attgctgaga attaaaatta
      961 tatcgggttt gtgcatacag gcttgatctc gtagtcggat agtaattcaa ttgatatgat
     1021 aattcagcgc tcggaatata gtacaattct gcatcattat aacctggtat aatatcgtaa
     1081 attccaaaat tggcttttgc agagaaatca tgctgccagt ttaattttac aggtttacta
     1141 taattgaatg atgcagaact tatcacaccg aaatatttag tctgaaagtc agaatttgtt
     1201 tcatcgtatg ctttacagta ataagtcggt gaaactttaa agcttaattc caaaccggaa
     1261 gaccggttga acaatgctcc ataatcccac atatcataca acgatgtaaa ataaggtgca
     1321 tccacattgt tcaatactcc tttgtttttc agataattat ccagcgttga aatctcatca
     1381 attctatgca gacggctatc caaaaaccgt ttgtttttta cggtagaaat aagctgagca
     1441 aattcaaaaa tttcctcttt atccatctca cgcttcagaa cattgtttct cttaaattcc
     1501 tccaatatat aaatagcctg ccgtgcatct tccacacgtt caattctgcc gataccccaa
     1561 ccaatctccg gaatgacgtt gacttccgat gtcttgtatg tagtaatatc aggaatttga
     1621 gttttggtta tcttctggta attaccatag taattgactg tggcattgta aatgaaaaag
     1681 tgctttccgg aataatataa tttgttaaac catcggacat agtcggaatt actaaaggag
     1741 aaattcttat cggtgaattt tgaatatccc agcccaacat caattccggt ttggaaagtg
     1801 ctgatttttt tacgtgtgtt aagcaggttg gaaaagttca gttgtgtacc ctgtaaattg
     1861 aaagaactgg cagttgtcgt gtctctgtaa ttagtcgcac taccattcat gttaaattgg
     1921 aattcaagtg agtttcgttt tatgtccgga gtgtaatact tgcttaaatc gaacccgtta
     1981 taatcatttt gagctgataa ttgggaaatc actaaaatta acaaagaaaa atacagggaa
     2041 aatcgtttca tgctgataga ttttattgtt atttactgcg tttaaatttg cattaaaaga
     2101 aaaaataagg atataaccat cctgaaacat tatttagctg attaagttta tattacatca
     2161 gcggactaaa caaccgggca aaggattctt tcagtttctg gcggcgtttt cggttattcc
     2221 atgcatcata agtgagttct tcacacttat ttaagtcatt cataaataat tcctttagtt
     2281 gaagggctgt tttggtatcg tagatgaatg catttgcctc aaagttttgg tcaaagctgc
     2341 ggtcgtccat gttggttgaa ccgatgatgg aaacaaaatc atccagcaca atggctttgc
     2401 tgtgcagaaa tcctttttta taaataaaca cccgtacacc
//
LOCUS       DAOPYB010000330         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_63016_length_2416_cov_8.047014, whole genome shotgun sequence.
ACCESSION   DAOPYB010000330 DAOPYB010000000
VERSION     DAOPYB010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63016_length_2416_cov_8.047014"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKH58_13685"
     CDS             <1..281
                     /locus_tag="PKH58_13685"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90122.1"
                     /translation="VTTSIGNLENASVNIYPNPSNGIFTISFGEIKSSAISLQIINTM
                     GAVVYEEKISSNSPTTKMLNLKELPEGIYSVIIQAYNQRLMKKMTIIK"
     gene            385..>2416
                     /locus_tag="PKH58_13690"
     CDS             385..>2416
                     /locus_tag="PKH58_13690"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNX90123.1"
                     /translation="MKKILLLSLLVSFGLFGFSQNNRVTVPKSLKNFAVQKAKAIDNS
                     LNVQTEVKHSPAKSVRTINEEEIGQTVYDLQSNQCTPYGRFVYYDDGTMGATWTMGTG
                     AYTDRGTGYNFFNGTTWGPEPTTRIENTKTGWTAYCGVGAQGEMNIAHVSGTAPLVVC
                     KRATKGTGAWTQSTFPGPSGHQLLWPRMVSGGTDHNTIHIIGLTAPVANGGTAYLGQD
                     GAVCYSRSTDGGVTWDKNNVVLEGTGSTDYVSFGGDAYSLVEPKGDNMAIIYCDNQND
                     FFVMRSSDGGDTWQKTIIWEHPYPHWNGTSAVDTFYCPDGSAHGAFDNNGLLHIVFGV
                     NRAMADGSSASWFPFVDGIAYWNENLPTWTGGTPYEQKYCLDPTTLEEQGSLIGWMQD
                     VNGNGQLDLVGTATSILGLYYLSPSSMPQLAIDENNMLYMFYSGITETFDNGTQQYRH
                     IWGRGSDDGGQTWKEFKDFTGDVLHAYDECVFPVIAAKTDDNVHCIYQGDNEPGLSVR
                     GDEDPAGDNYIRHLFPSKDEFGLVPADNYTVDGTVTYPKSTPVPLSGVEIALKDNGGN
                     VIQTATTDASGAYSFVEVPAGDYTLAPTTTKPWGGVTAGDVLLYKKHIAGISALDGIF
                     LASGDVNASGSLTAGDVLIIKKRIAGIISSFTVGDWIFNNTPLAVTSNTTADF"
BASE COUNT          718 a          473 c          500 g          725 t
ORIGIN      
        1 atgtaacaac ttctatcggc aaccttgaga atgcttctgt aaatatttat cctaatccga
       61 gcaatggaat attcacgatt agctttggcg aaataaaatc ttcagcaata agtctacaaa
      121 taattaatac gatgggtgca gtggtttacg aagagaaaat ttcttcaaat tctccaacta
      181 ccaaaatgct aaatttaaag gaattgccgg agggtattta ttctgttatt attcaagcgt
      241 ataatcaaag gttgatgaaa aaaatgacta ttataaaata atcgtctgtt tttattaata
      301 aagttttaag tttacttttt ttttttattt ttgcaattta gtaatattaa aatccattta
      361 taaacaaaaa tttaaattaa cgacatgaaa aaaattctac tattaagctt actcgtaagc
      421 tttgggttat tcgggttttc ccagaataac cgggtaaccg tgccaaagtc attaaaaaac
      481 tttgccgttc aaaaagctaa agctattgac aattcattaa atgttcagac tgaagtaaaa
      541 cacagccccg ccaaatctgt acgaacaatt aatgaagaag aaattggtca gacagtttat
      601 gatttacagt ctaatcaatg caccccttat ggacgatttg tttattatga tgacggaacc
      661 atgggcgcta catggacaat gggaacaggc gcttatacgg accgtggtac aggttacaat
      721 ttttttaacg gcacaacctg gggaccggaa ccaactacaa gaattgaaaa cacaaaaacc
      781 ggatggacgg cttattgcgg cgttggagca caaggtgaaa tgaacattgc tcatgttagc
      841 ggaaccgctc cattagtagt ttgcaaacgt gctaccaaag gtaccggagc atggacacaa
      901 tctacttttc caggaccaag cggtcatcaa ttattatggc cacgtatggt ttctggcgga
      961 accgaccata atactattca tattattggt ttaacggctc ctgtagccaa cggcggtact
     1021 gcctatttag gacaggacgg cgctgtttgt tattcccgtt ctaccgatgg tggagttacc
     1081 tgggataaaa acaatgtagt tttagaaggc acaggttcta ctgattatgt ttcttttggc
     1141 ggagatgctt attcattggt tgaacccaaa ggtgataata tggctattat ttattgcgat
     1201 aatcaaaatg atttctttgt aatgagatca tccgatggtg gagatacctg gcagaaaact
     1261 attatctggg aacatcctta tcctcattgg aatggaacat ccgcagttga tacattctac
     1321 tgccccgatg gctctgccca tggtgcattc gacaataatg gtttgttaca catcgtattt
     1381 ggtgttaacc gtgccatggc tgatggttca tcggcttcat ggtttccatt tgtggacggt
     1441 attgcatatt ggaacgaaaa ccttccaacc tggactggtg gaactccata tgagcaaaaa
     1501 tattgcctcg atcctactac ccttgaagaa cagggaagcc tgattggctg gatgcaggat
     1561 gtaaacggca acggacaact cgatcttgta ggaacagcaa cttctatttt gggattatat
     1621 tatttaagtc cttcatccat gcctcaactt gctattgatg aaaataatat gctgtatatg
     1681 ttttattcgg gtattaccga aacttttgat aacggaacac agcaataccg tcatatctgg
     1741 ggacgcggtt cagacgacgg cggacaaacc tggaaagaat tcaaagattt taccggcgat
     1801 gttcttcatg cttatgatga atgtgttttc cctgtgatag ctgctaaaac cgatgacaat
     1861 gttcattgca tttatcaggg cgataacgaa ccaggattgt cagtaagagg cgacgaggat
     1921 cccgctggcg acaactacat ccgtcatctt ttcccatcca aagatgaatt tggcttagtg
     1981 cctgctgaca actataccgt tgacggcaca gttacttatc ccaaatctac ccccgttcct
     2041 ttgagtggtg tagaaattgc cttgaaagat aatggcggta acgtaattca gacagccact
     2101 accgatgcat ctggtgctta ttcatttgtt gaagttcctg caggtgatta tactctcgct
     2161 ccaactacaa ccaaaccatg gggcggcgta accgcaggtg atgttttatt gtacaaaaaa
     2221 cacattgcag gtatcagtgc tttagatgga attttccttg cttcgggcga tgttaacgca
     2281 agcggaagcc ttaccgcagg tgatgttttg attattaaaa aacgtattgc cggtatcatc
     2341 agttcattta ccgttggcga ttggattttc aataatactc ctttagctgt tacgtctaat
     2401 accacagccg atttca
//
LOCUS       DAOPYB010000331         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_63249_length_2410_cov_7.369002, whole genome shotgun sequence.
ACCESSION   DAOPYB010000331 DAOPYB010000000
VERSION     DAOPYB010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63249_length_2410_cov_7.369002"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKH58_13695"
     CDS             complement(<1..331)
                     /locus_tag="PKH58_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNX90124.1"
                     /translation="MAIIKPFKGIRPPKDLVEKVASRPYDVLNSEEARQEAEGNQYSL
                     YHIIKPEIDFAPGTDEHDEKVYLKAAENFAKFRENGWLVQDKAEKYYVYAQTMNGRTQ
                     YGLVVCAS"
     gene            complement(486..1106)
                     /locus_tag="PKH58_13700"
     CDS             complement(486..1106)
                     /locus_tag="PKH58_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90125.1"
                     /translation="MKTTIFKISVLILSLSPIVFSCSDKENNSPNISKTKGSGNVHYD
                     NTINSLNGGVLKINSAAQTYILYDLILFSTNYNFDLNTGKLTHGNFSPLNYVKFTICL
                     SKTTADLNGDGIINSNDMSASDVSLPLGKYSFNNAYVNSTFTGGEFSKNDPATKLHII
                     QSGTLEIKHENNLYAINYDGKDTDGVSLYCDYKGKIGFVYNGKYIN"
     gene            1264..>2410
                     /locus_tag="PKH58_13705"
     CDS             1264..>2410
                     /locus_tag="PKH58_13705"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNX90126.1"
                     /translation="MESVREIYRIGHGPSSSHTMGPRFAAERFNNENPSAISFRVSLY
                     GSLAATGKGHLTDVAIAESLLPKSVEFIWLPEIVLPFHPNGMKFEALGAEGKVLKDWL
                     VYSVGGGKIMDEETAADEVKIFEHSTIGEIMPWVERRGQMYWEYVNERENSDIWDYLN
                     EVWKTMQNAIHEGLETDGVLPGELGLRRKAASYWIKAKGYKQSLQSRCLVMAYAMAVS
                     EQNASGGTVVTAPTCGSSGVLPAILYHFKLNHSMSDRHILQALATAGLFGNVIKKNAS
                     ISGAEVGCQGEVGSACAMASAAACQIFGGSPQQIEYAAEMGLEHHLGLTCDPVCGLVQ
                     IPCIERNSFAALRALDANLKSMLSDGKHIISFDKVVETMRNTGHDLPS"
BASE COUNT          686 a          467 c          560 g          697 t
ORIGIN      
        1 cactggcaca aaccaccagt ccgtactgag tgcgtccgtt catggtctgg gcatacacgt
       61 aatacttttc agctttgtcc tgaacgagcc agccgttttc acggaatttg gcaaaattct
      121 ctgcagcctt cagataaacc ttttcgtcat gctcgtctgt gccgggagcg aaatctattt
      181 caggtttgat aatgtgataa agcgagtact gatttccttc tgcttcctgt cttgcctctt
      241 ccgagttgag tacatcgtaa gggcgtgacg ctactttctc tactaaatct tttggtggac
      301 gaatgccttt gaatggtttg ataatagcca ttttttatta tgaattagga ttatgaatat
      361 ttggacaaat ttacgaaaaa tataagaata aattattttc agaatttaaa aagaactgaa
      421 gtttgtaatt aattgtatct ttaatcttag gttgattgtt actacggtga gggagatata
      481 ctttatcagt ttatatattt accattatac acaaaaccga ttttaccttt atagtcacaa
      541 tatagtgaaa caccatcggt gtctttaccg tcgtaattaa ttgcatacag gttgttttcg
      601 tgtttgattt caagtgtgcc tgattggata atgtgtaatt tagtagcagg atcatttttt
      661 gaaaattctc ctcccgtaaa tgtggaattt acataggcat tgttaaagga gtattttccc
      721 agaggcagtg atacatcgga agctgacata tcatttgagt tgataattcc atctccgttt
      781 aaatctgctg tggtttttga taggcaaatg gtgaatttaa cataattaag tggtgaaaag
      841 ttaccgtgag ttagttttcc cgtattgaga tcaaaattgt aatttgttga aaacagaatt
      901 aaatcgtaaa ggatataagt ttgtgcggca gaattaattt taagtacacc tccattgagt
      961 gaatttatag tattgtcgta gtgaacgttt ccggagccct ttgttttgga aatatttgga
     1021 ctgttgtttt ctttatcgga acacgaaaaa acaataggcg acagactcaa tattaacact
     1081 gagattttaa aaatagttgt tttcatatgt attacgtttt tatggatttg aaagacaaat
     1141 ttataaaaag gttttgagtt tcaaagttca aacctgcaaa actttcaccg gataaaatta
     1201 agacactgca ccaatgcatg aacggaagtg ttttttatta tctttgcata aaacaatgta
     1261 actatggaat cagtcaggga aatatacaga atcggtcacg gaccatcgag tagccacacg
     1321 atgggacccc gttttgccgc cgaacgtttt aataacgaaa atccttccgc catatcattc
     1381 agggtaagtt tatatggcag cctggctgct accggcaagg gtcacctcac cgacgtagct
     1441 attgctgaat cattactgcc aaaatccgta gaattcatct ggcttccaga aattgtactc
     1501 ccgtttcatc ccaacggaat gaaatttgaa gctctcggcg cagagggaaa ggtgctgaaa
     1561 gactggctgg tgtacagtgt gggtggtgga aaaatcatgg atgaagaaac ggctgctgac
     1621 gaagtgaaga tattcgaaca cagtacaatc ggtgaaataa tgccctgggt ggaacgccgc
     1681 ggacagatgt actgggagta tgtgaacgaa agagaaaaca gcgatatctg ggattacctg
     1741 aatgaggtct ggaaaacgat gcaaaacgct attcacgagg gactggaaac cgacggtgta
     1801 cttcccggag aactgggatt gcgacggaaa gctgcttcgt actggatcaa ggcaaaaggt
     1861 tacaagcaaa gtctgcaaag ccgctgcctg gtgatggcat acgcgatggc ggtgtcagaa
     1921 cagaatgcca gtggaggaac agtggttact gctcccacct gcgggtcttc gggggtgttg
     1981 ccggctattc tctatcattt caagctcaac cattccatgt ccgaccgtca tatccttcag
     2041 gcgcttgcca cagccggttt gtttggtaat gtgataaaga aaaacgcgtc catttcggga
     2101 gccgaagtag gatgtcaggg tgaagtggga tcagcctgcg cgatggcttc ggctgccgcc
     2161 tgtcagatat tcggcggaag cccgcaacag atagaatacg cggcagagat gggactggaa
     2221 catcacctgg ggctgacctg tgacccggta tgtggtctgg ttcagatacc ctgcatcgaa
     2281 cggaattcgt ttgccgcgct gcgcgccctt gatgccaacc tcaaatccat gctttcggac
     2341 ggaaaacaca ttatcagttt cgataaagtg gtggaaacca tgcgcaacac cggacacgat
     2401 ttaccatcac
//
LOCUS       DAOPYB010000332         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_65697_length_2352_cov_10.220723, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000332 DAOPYB010000000
VERSION     DAOPYB010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65697_length_2352_cov_10.220723"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PKH58_13710"
     CDS             complement(<1..1190)
                     /locus_tag="PKH58_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HNX90127.1"
                     /translation="MKRTLRYLATACMLAVISVSISYGQAPQKFNYQAVCRDLSGNII
                     ANQAVTLRMTIHDISATGAVLYQETHAVTTNNFGLVNVAVGGGTVVSGTFATITWGTG
                     AKYLQIEVNTGSGYNSVGAPQLLSVPYALYANQANVLGATGPTGPIGATGPIGATGPI
                     GPAGPIGPTGATGVMVAGSNMQTLRHNGTDWVANSILTNNGANIGINAAPQVGKKLYV
                     YRPSGNFGPDSSTIYAYRYGVSGTPASGGTSWARSGVDAAIKGYSFYGNNFTAGVGGY
                     NYLDYANSAGVIGANSTASTFGALTFKDDLNTIWAGYFYGNAKVTGTVTIAGGTPDAN
                     KVLTATNTAGDAVWQSMAALLPVGTSGQTLRHDGTNWIANSVLFNDGTNVGIGTTTPG
                     TKFEV"
     gene            complement(1209..1718)
                     /locus_tag="PKH58_13715"
     CDS             complement(1209..1718)
                     /locus_tag="PKH58_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90128.1"
                     /translation="MRKLLFFALLTMLSASLIAQSMERWVVSGAGGIYLDNSNNFEMD
                     YTIGEPIITTASGASIILTQGFQQPFFIMTSLPETIDNSAEWSFYPNPVAEHLFIVND
                     KPGQGTYVIQLFDMMGQLLVESSRKSAGNTSLTLMLDFDGYATGNYFVRLLYQHKALK
                     TFKIIKINQ"
     gene            2181..>2352
                     /locus_tag="PKH58_13720"
     CDS             2181..>2352
                     /locus_tag="PKH58_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX90129.1"
                     /translation="MKRTTNDLAGESLWHLLIMYSVPAIIATAAASIYNIIDRIFIGQ
                     GVGPLAISGLALT"
BASE COUNT          669 a          532 c          487 g          664 t
ORIGIN      
        1 acttcaaatt tggtgcccgg ggtcgtagtt ccaataccca cattggttcc atcattaaac
       61 aagacactgt ttgcaatcca gttggtgcca tcatgcctta aagtctgccc cgaggttcca
      121 acaggtaaca gggcagccat gctctgccat actgcatcgc cggccgtatt tgtggctgtg
      181 agtactttgt ttgcatcagg agtgccgcca gcaatagtaa cagtaccggt aaccttggca
      241 ttcccataaa aataacctgc ccagattgta ttaagatcat ctttaaaagt taaagcccca
      301 aacgttgatg ccgttgaatt agcaccaatg accccggctg aattagcata atccaggtaa
      361 ttatatcccc cgacgcctgc cgtaaagttg tttccataaa atgaataccc tttgatcgcg
      421 gcatcaacac cagaccgtgc ccatgaagta cctccactgg caggagtacc agagactccg
      481 taccgatacg cataaatcgt cgaagaatcc ggcccaaagt ttccggatgg acgataaaca
      541 taaagttttt ttcctacctg aggcgctgca ttgatgccga tatttgcacc gttattagta
      601 aggatactgt ttgccaccca atcggtgcca ttatgcctga gggtttgcat atttgagcct
      661 gcaaccatta ctccggtagc tcctgtcggg ccaatggggc ctgcggggcc aatggggcct
      721 gtagcgccaa tagggcctgt agcgccaata gggcctgtag ggcctgtagc accaagtaca
      781 ttagcctggt tggcataaag agcataagga acgcttaaca attgcggtgc acccacactg
      841 ttgtatcccg atcccgtatt aacctcaatc tgaaggtatt tggcgccggt gccccaggta
      901 atggtcgcaa aagttccgga aaccactgtg ccaccgccaa cggccacatt aaccagtccg
      961 aaattgttgg tggtaacggc atgtgtctcc tgatacaaca ccgctcccgt ggcactgatg
     1021 tcgtgtatgg tcattcgtaa ggtcacagct tgattggcaa tgatattacc cgataggtcg
     1081 cggcataccg cctgataatt aaatttctgc ggcgcctgcc cgtagcttat actgactgaa
     1141 ataacagcca gcatgcaagc tgttgctaaa taacgtaatg ttcttttcat aatataatat
     1201 tttaatggtt attgattgat tttaattatc ttaaatgtct taagcgcctt atgctgataa
     1261 agcaggcgta caaaataatt tcctgtagca tacccgtcga aatcgagcat caacgtcagg
     1321 gatgtgttgc cggctgattt ccggctggac tcaacaagca attgccccat catatcaaaa
     1381 agctggatta cataagtccc ttgccctggc ttatcgttca cgatgaacaa atgctccgct
     1441 accggattgg gataaaaaga ccactccgca gaattatcaa ttgtttcagg cagggaagtc
     1501 atgataaaaa aaggctgctg aaacccctgt gtcaggatta ttgaagcgcc ggaagctgta
     1561 gtaataatgg gttcaccaat ggtatagtcc atctcaaaat tgttactatt atcgagatag
     1621 ataccaccgg cccctgaaac aacccagcgt tccatggact gtgctattaa cgatgcggac
     1681 agcatcgtta atagtgcaaa aaataataat tttctcatac acgtactttg ttttaaattt
     1741 tcagtaaata taatatattt taagttaatt ttcaagtatt ttaacatttt ttatgcaaag
     1801 aagtgcgaat cattctcaca ccaataacaa cccagagcat aagtgtccca tttaaatttc
     1861 cggggcaata aataagaatc actgttcgag gaaaaaatgt tgtgaattcc ccttacaaat
     1921 tcattttttc atttattatt aaaatccgta ttgacagcca attgaaggta tatatggata
     1981 atatatttta aaaaacccgg gtttattttt cgtaaatttt attttataac gataacccat
     2041 acaaatgatt agcgtttgtc atgtatgcca atcagcgatt ccggctgttg taacagtata
     2101 ttatgtttgc ttgttttcgg tcacgctgat gtatatttgc agatattttc attcctgaaa
     2161 taccatagtc aaaagcaaca atgaaaagga caaccaatga tctggccggc gagagcctct
     2221 ggcatttgtt aataatgtat tcggttccgg cgattattgc tactgctgct gcttcaatat
     2281 ataatatcat cgacagaatc tttataggac aaggtgtagg gccgctggcc atctcaggat
     2341 tagcgctcac at
//
LOCUS       DAOPYB010000333         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_67062_length_2320_cov_9.600000, whole genome shotgun sequence.
ACCESSION   DAOPYB010000333 DAOPYB010000000
VERSION     DAOPYB010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67062_length_2320_cov_9.600000"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1205..2248
                     /locus_tag="PKH58_13725"
     CDS             1205..2248
                     /locus_tag="PKH58_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90130.1"
                     /translation="MKTLISKTVIILAMTSPAITVLAATTVYKTDYISVADENSKQTS
                     HFLNTTAFTDTVSSVTGRKDRNVKGKGEGINEAGIKRADSQRKGWDGTVKGGNIQEKS
                     INGINNGMPNRISMNITVAKQTQGATFGEKVNAGLQTAGKINITLVEDGCVVLFSNND
                     GYRVYTENQCIRKLTAEEYAKINAGLHATGSALAQGASLLGGALPGGAIVSAAISSVG
                     NLAGGAGGGAASASYAATGRITGDGKDNDCDGKADIVAEVTEFTGEISNKKLDKLSTA
                     ERVVWKGKDDDCNGTAELTDGDYELSFIVIDKATSGLKDTLKTQVRIQFSSISNVLKT
                     KHDTAKNAIGNVR"
BASE COUNT          809 a          434 c          443 g          634 t
ORIGIN      
        1 aagatatcgt tcccgaactg attgaacgcg tgaaagccgg atttacattc ggagttgaac
       61 cggtgaaaaa ataattaact cacatacttt acgaaaagac ggatgaattc aacatccgtc
      121 tttttttgtt tacgtgcgtt cgatcttatt ttgtccatta ggacttaaat atcaatagaa
      181 agaatgtttt ttgtttaatg attatcagca aagcgtccaa tataaacgct caattttgat
      241 aaagatgtta tttagggacg tagtcctgaa atcatcgtag aaatgaattt ttacagcaaa
      301 tttgccgcgt tagggtgata tctttttgat ttattggttt aatcgttaat cgtgtgaaat
      361 aagaaaataa tccaacacca aagatagcaa aatttaaagg gtcagagacc cgtaaaattt
      421 gtagaaacga gaatcacccg caacccataa gggtcggaga cccgaaatat aaaataacgt
      481 tgaatgggtg taaatacaat gatattccgg tgcgccgcac cttaaaaaac aactcgctac
      541 gctatagcta caaatgtttc gcggctctgc atccaccccc ttcctaagca taaacttcat
      601 gtcatactgt catctaagcg aagcaaagaa tctacacatt ttctctggtc aacagtgatt
      661 cttaataaaa taatatttca cacgattaac gattaaaccg atttattcaa tatatagatg
      721 tcagagctac gctcctaaaa cataatttca ataaacaggt attattacgg ataatcattt
      781 tttttcattt cccgtaagga aaggatatat ttatccccta acgtggaatt aaaaattatt
      841 ttcaacctat ttctattgat atcaattccc aacggaaatt cagactattg tatttgaaag
      901 ggataaatga tttttttatc gaactcacgt taatttaaaa gacggatgaa ttcaacatcc
      961 gtcttttttt tgttaaataa agtttatatc aaattaaaga attatttcta taatcaactc
     1021 atttaaaaac atctcagatg ccctaaacat cgattatccc gttaaaaagc cctggctttt
     1081 tcggatattc aacaaatctc aacttgttaa tttttcagtg taaacaaaaa ctaaccgaac
     1141 aaaataaata tgttcatgtt atagttatta aatttagaat tgttccattt aacaatgaaa
     1201 aatcatgaaa accctgatta gcaaaacagt aattattttg gctatgacaa gcccggcaat
     1261 aacagttctt gcagctacaa ccgtttacaa aacagattat atttcagttg cagatgaaaa
     1321 cagtaaacaa accagccatt ttctgaatac aaccgccttc acagacacgg taagctctgt
     1381 taccggacgc aaagaccgga acgttaaagg aaaaggagaa ggaattaacg aggcaggtat
     1441 aaaacgagcc gacagccagc gcaaaggttg ggatggcacc gtgaaaggtg ggaatattca
     1501 ggaaaaaagc attaacggaa ttaataacgg tatgcccaac cgcatttcga tgaatatcac
     1561 cgtagcaaaa caaacgcagg gagccacttt cggtgaaaaa gtgaacgccg gactacaaac
     1621 cgccggtaaa atcaatatta ctttggttga agatggttgc gtggtacttt tctccaacaa
     1681 tgatggttat cgggtttaca ccgaaaatca gtgtatccga aaactcaccg cagaagaata
     1741 cgcgaaaata aatgccggac tgcatgctac cggaagcgca ctggcgcagg gtgcatccct
     1801 gttgggtgga gcattgccag gaggagccat tgtttcggca gctatttcga gtgttggtaa
     1861 tctggcagga ggtgcaggcg gaggagctgc ctcagcttcc tatgcagcaa caggaagaat
     1921 aaccggtgat ggaaaagata acgattgtga tggcaaagct gatattgtgg ctgaagtgac
     1981 agaatttacc ggagaaattt ccaacaaaaa gcttgataaa cttagcactg ctgagagagt
     2041 ggtttggaag ggaaaagatg acgattgcaa cggcacagcc gaactcacag atggtgatta
     2101 tgaactttca ttcatagtaa ttgataaagc aacatcagga ctgaaagaca cgctgaaaac
     2161 ccaggtcaga attcagttca gcagcatcag caatgtcctg aaaaccaaac acgacacagc
     2221 aaagaacgcg ataggcaatg tcagataata atgaatataa gaaaaaaccc ttaccttaat
     2281 ttttcatgtt tgtttattcc tcgatttcgt aaatccaatt
//
LOCUS       DAOPYB010000334         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_67118_length_2319_cov_7.709806, whole genome shotgun sequence.
ACCESSION   DAOPYB010000334 DAOPYB010000000
VERSION     DAOPYB010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67118_length_2319_cov_7.709806"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..140
                     /locus_tag="PKH58_13730"
     CDS             15..140
                     /locus_tag="PKH58_13730"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90131.1"
                     /translation="MSIEAVKTLYVRIAGSGNVMYKGNPQIDQSIAGSGKVKNVR"
     gene            complement(320..1774)
                     /locus_tag="PKH58_13735"
     CDS             complement(320..1774)
                     /locus_tag="PKH58_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX90132.1"
                     /translation="MTTNRRNFLRNTMMGAGLIASGFAGQAASVKIESSTKSQGSSGY
                     QQKFNMSGYAAPKIDKVRIGFVGLGQRGPGAVERMSYIDGVEIVALCDQYPDRVEAMQ
                     KLLEKQGLPRAKGYSGSKDSWKAMCENPNIDLIYITTPWSWHTPMAVYAMEHGKHAAT
                     EVPAAVTIDECWQLVETSEKTKKHCMMLENCCYDFFELLTLNMARQGFFGDIVHGEGA
                     YIHNLIDLNFKKDGYAEMWRLKENQHRNGNLYPTHGLGPICQVMNINRGDQMEYLTSM
                     SSADFQMAKRAKDEAAKDSFYNEFTTDHYRGNMNTTTVRTYMGRTIMIQHDVTSTRPY
                     SRIHLVSGTKGVASKWPDPARISTGHEWLKDEEFKALQDQYTPEIVKKIGEMAKKVGG
                     HGGMDFMMDWRLIDCLRNGLPLDQNVYDAALWSCIAPLSEKSVANRSNSVDIPDFTNG
                     AWKTNAPVELTLKGGGTTGVREKAVGGSKQLKVQ"
BASE COUNT          595 a          481 c          505 g          738 t
ORIGIN      
        1 ccggatctgg agatgtgagc atcgaagctg tgaaaacttt gtatgtgcgg attgccggat
       61 cgggaaacgt aatgtacaag ggaaatcctc agatagacca gtctattgcc ggttcgggca
      121 aggtgaaaaa tgtaagataa aacaatttaa attagttgtc aggaaagtta ttgaggaatt
      181 cagatcgctc tgtttgttat aacggattgt aaatttgcca gttcttatag ttgatacaga
      241 aatatcccaa gacggttgat taaaaaaatc ccgaagaatt tcgcttcccc gggacttcga
      301 tgtcgtttct attttatggt tattgaactt ttagctgttt cgatcctcca actgcttttt
      361 cgcgcacacc ggtagttccg ccgcctttta aggtcaattc aaccggagca ttggttttcc
      421 aggcaccatt agtaaaatca ggaatatcaa ccgagttcga acggttagcc actgattttt
      481 cactcagcgg agctatgcaa ctccacagcg ctgcatcata tacgttttga tcaaggggaa
      541 gtccattgcg caggcagtcg attaaacgcc agtccatcat aaaatccatt cctccatggc
      601 cgccaacttt cttggccatc tcgccaatct tttttacaat ttcaggagta tattgatcct
      661 ggagtgcttt gaattcttca tctttcagcc attcgtgtcc ggttgaaata cgggcgggat
      721 caggccattt actggccaca cccttggttc cgcttacaag gtgaatacgc gagtatgggc
      781 gggtgctggt cacatcgtgt tgtatcatta tggtgcgacc catatatgtg cgtaccgttg
      841 tggtattcat gtttccgcgg tagtggtcgg tggtaaactc attgtagaaa ctgtctttag
      901 ctgcttcatc ctttgctcgt ttggccattt ggaaatcggc cgacgacatg gaagtgaggt
      961 attccatctg gtcgccgcgg ttaatgttca tcacctggca gattgggcct aacccgtggg
     1021 tagggtagag gttcccgttt cggtgctgat tttccttgag tcgccacatt tctgcataac
     1081 catctttctt gaaattcagg tcgatcagat tgtgaatgta tgcaccttcg ccgtgaacaa
     1141 tgtccccgaa gaaaccctgt cgggccatgt tcagcgtcag aagttcaaag aaatcgtaac
     1201 agcaattttc aagcatcatg cagtgcttct ttgtcttttc tgatgtttca accagctgcc
     1261 agcactcgtc gatggtgact gcagccggaa cctccgttgc tgcatgcttt ccgtgttcca
     1321 tagcgtaaac ggccattgga gtgtgccacg accagggtgt ggtaatatat attaaatcga
     1381 tgttcggatt ttcgcacatg gctttccatg aatctttgct tccagagtaa cctttggcgc
     1441 gtggtaaccc ttgtttttcg agcagtttct gcatggcttc aacccggtcg ggatattggt
     1501 cgcacaaagc aacaatttca acgccatcga tgtacgacat gcgctcaaca gcacctggcc
     1561 ctcgttgccc aagaccgaca aagccaattc gcactttgtc tatttttggc gctgcatacc
     1621 ccgacatgtt aaatttttgc tggtagcccg atgagccttg agatttagtt gatgactcaa
     1681 ttttcaccga agccgcctga ccagcaaatc cactggcaat taatccggct cccatcatcg
     1741 tgttccggag aaaatttctt ctgtttgttg tcataattat tttgttcagg ttagactaaa
     1801 aatcaaaaat gcagtttatt gcccgtgaaa ttaatggttt ttatcattta attggattga
     1861 ataagtttgt tgtgaaattt tatttgcggc cagatttgaa gatattgagc tgtgtctcac
     1921 agttgttttt tatgatacaa attgggctga ttattgcact ggataggaaa aagagctcat
     1981 tttttgttca aaatgtggca atttaactga tgtttttgat ggctgattaa tcaaattctg
     2041 ttagagcgct gtttattttt aatctaaata gtacaatctg cctgtaattt ttggaagatg
     2101 aaaatttgca aaaatttctg caaatatttg ataataaatg ctgtggctga ttttattgag
     2161 attggttctg aacaaaaata ggaatctatt gtgttaggca tcaacgagta acatatatgc
     2221 taccagagaa atgcttctga aaattttttt cttctgttaa aaatacttaa attttcatac
     2281 ctttaaatcg aattgcgcca atctacacat taaaaaagc
//
LOCUS       DAOPYB010000335         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_67382_length_2313_cov_6.099203, whole genome shotgun sequence.
ACCESSION   DAOPYB010000335 DAOPYB010000000
VERSION     DAOPYB010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67382_length_2313_cov_6.099203"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2143)
                     /locus_tag="PKH58_13740"
     CDS             complement(<1..2143)
                     /locus_tag="PKH58_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HNX90133.1"
                     /translation="MKASRKTGNQYNLKKSTVLVIIFILFCHIGSATKYYVATNGNDS
                     NSGAINSPFANWEKLGSVLAAGDTAFIRGGTYRTAKSASSEFFCLWNGLVGTQSDSIK
                     IWAYPGETPIFNLDNITPTHAVAYGICLLNSQYIYIKGLRVTGLAQNVSGNIIYGINT
                     INSGNCKIEKCEVDHIGGYGFAFSGTTHKMIIKNCDTHHLDDLYSSTPHGGSNGFSIT
                     GDNSVDSITFYGCRAWFVSDDGWDFYGSDGFFIIDNCWSFWNGYDENFNILGDGSGYK
                     LGPASSSNLTSVKRIIRNSLAVENRDYGFNCNEANYNIPMNVYNNTSYKQSNNTGFNF
                     AWGATGAVTTLRNNISYLDHVAYEGKSSNIQDHNTWNGGVTVSNADFVGLDSSQLLSA
                     RQSNGNLPTITFLHLSSGSDLIDAGTNVGLPYYGTAPDMGTFEFNSYSNQPPVIQNQS
                     FQLNENSPNGTVVGTVVATDPNAGQTLTYSILSGNTNDAFSINSSTGVLSVNSSSALN
                     YEVTPSFALVVKVQDNGNPPANSQATITVALLNVNEPPIAYDQTFNVIEASANGTIVG
                     YVGALDPDAGQTLSWAIISGNTSNAFAINSTTGALSVNNSSALNYQTNPIFNLIVRVT
                     EVNGANLFDDASVSINVLQGTNQAPVILNQSFSINENSSNGTLVGNVAASDPDAGQTL
                     TYSILSGNTGSAFAINASTGAITVNTSSVLNY"
BASE COUNT          697 a          449 c          455 g          712 t
ORIGIN      
        1 cgtaattcag aacagatgaa gtattgactg tgattgcgcc tgttgaagca ttgatagcga
       61 aggcactgcc tgtatttcct gacaggatag aatacgtaag tgtctgtccg gcatcaggat
      121 cagaagcggc tacattaccg accagtgttc cgtttgagga gttttcgtta atgctgaatg
      181 attggtttaa tattactgga gcctgattgg tgccctgtag gacattaata gacactgaag
      241 catcatcaaa tagattggct ccatttactt cggtaactct tacaatgagg ttgaatattg
      301 ggtttgtctg gtaattgagt gctgatgagt tatttaccga cagggctccc gtggttgagt
      361 tgatagcaaa tgcgttgctt gtattgccgg aaataattgc ccaggaaaga gtttgacctg
      421 catcaggatc gagagctccc acatagccta caatggtccc gttagcggag gcttcaataa
      481 cattgaatgt ttgatcataa gcgatgggtg gctcgtttac attgagtaat gctacggtaa
      541 tagtagcctg gctgtttgca ggtgggtttc cattatcctg tacttttact actaatgcaa
      601 acgaaggagt tacttcataa ttcagtgctg aagagctatt tacactcaga accccggttg
      661 aggaatttat ggagaaagca tcgttagtat tacccgagag gatggaataa gtaagtgttt
      721 gccctgcatt ggggtctgta gcgacaacag tacctaccac agttccatta gggctgtttt
      781 cattcagttg aaaactttgg ttctggataa ccggaggttg attgctatag ctgttgaatt
      841 cgaaagtacc catatcagga gcagttccat aatatggtaa cccgacatta gtaccggcat
      901 ctatcaggtc agaacctgag gataaatgca aaaatgtaat tgttggtaga ttaccatttg
      961 attgccttgc acttaataac tgtgatgaat caagaccgac aaaatcggca tttgaaacag
     1021 tcacaccacc attccaggta ttatgatctt gaatattgct tgattttcct tcataagcaa
     1081 catgatctaa ataacttatg ttatttctta atgtagttac cgcacccgtg gcgccccagg
     1141 caaaattgaa tcctgtatta tttgactgtt tataagaggt gttattataa acattcatag
     1201 ggatattgta atttgcttca ttacaattga agccgtaatc cctgttttca actgccagac
     1261 tatttctgat aattcttttt actgaagtaa gattgctcga tgatgccggg ccgagtttat
     1321 aaccacttcc atcgcccagt atattgaaat tctcatcata gccattccaa aaagaccagc
     1381 agttatcaat aataaaaaaa ccatcagaac cataaaaatc ccagccatca tctgaaacga
     1441 accaagcacg acacccatag aaagttatac tgtcaacgga attatctcca gtaatactaa
     1501 acccattcga tcctccgtgt ggagtagaag aatacaagtc atccaaatgg tgggtatcgc
     1561 aattctttat gatcattttg tgtgtggtgc cagaaaacgc aaaaccatag ccgcctatat
     1621 gatcaacttc acacttttca attttacaat ttcctgaatt aattgtgttg ataccataaa
     1681 ttatgtttcc tgaaacattt tgcgctaaac cagtaactcg taatcccttt atataaatat
     1741 actggctatt gagcaaacaa attccataag caacggcgtg tgtaggagtg atgttatcaa
     1801 ggttgaatat aggggtttct ccaggatatg cccatatctt aatcgaatca gattgagtac
     1861 caactaatcc attccacaaa cagaagaatt ctgaacttgc cgattttgct gttctgtaag
     1921 tacctcccct tataaatgcc gtatcacctg ctgctaaaac tgagcccagt ttttcccaat
     1981 ttgcaaaagg gctattgatt gctcctgaat tactatcatt tccatttgtt gctacatagt
     2041 atttagtagc tgatccaata tgacaaaaga gaatgaagat gattactaat actgtacttt
     2101 tcttaaggtt atattgattc ccggtttttc gagaagcctt caataagaat gataaagtga
     2161 caatcaaggg caaacaaaag ttgccgctag caatactagt tcggtgattt tccaactttg
     2221 tagtattaat aataatttta aaataaagca acagaattac ccattgcact ttattgcagg
     2281 ttgtgtcagt tcaacacgct gcaaaattct tta
//
LOCUS       DAOPYB010000336         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_67838_length_2303_cov_9.507117, whole genome shotgun sequence.
ACCESSION   DAOPYB010000336 DAOPYB010000000
VERSION     DAOPYB010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67838_length_2303_cov_9.507117"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PKH58_13745"
     CDS             complement(<1..1049)
                     /locus_tag="PKH58_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNX90134.1"
                     /translation="MQRVHFIAIGGAAMHNLAIALSKKNEYEVTGSDDEIFEPSYSRL
                     KENGLLPREMGWFPERIHKNLNAVILGMHATEDNPELIRARELGIHIYSFPEYLYQQT
                     RSKTRIVVGGSHGKTTTTAMILYVLKKCKINADYMVGAQIDGFDNMVKLSFESRIAVF
                     EGDEYLTSTLDRRPKFHLYKPHIAILTGIAWDHINVFPTFEIYVDQFRKFIDSMETQG
                     RLIYYNGDENLNMLANEAENSRRDVVAFPYNTPKYEIRDGVTYVKTRKGDIALKIFGE
                     HNLQNMEAAHLACRQIGVMDDQFYEAIADFPGASNRMQKIVDKPGKTAFKDFAHSPSK
                     LKATVNAARKQYPDR"
     gene            1133..1975
                     /locus_tag="PKH58_13750"
     CDS             1133..1975
                     /locus_tag="PKH58_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX90135.1"
                     /translation="MKLYKIEAGTFLADGGAMFGVVPKRVWQKRYPCSDENFCILAMR
                     CLLVDTGDRLILVDSGTGEKQLDYLKYYRFKGVVNFETELNKLGYSCSDVTDVILTHL
                     HFDHCGSCTRFNEDHSEIILTFPNATYWVGKAQWENFLNPNVREGDSYFKENMLPVME
                     AGKLRLVSEDMPLDDKVELKLYHGHTAGQIAPCFNLGDKTLVFAGDVIPIMASLPVAW
                     VSAYDTFPITSMEDKVKMLDEAAEKHQILFFEHDNYNECCTVKKINGKYRVEKILTLD
                     EVMK"
     gene            complement(2086..>2303)
                     /locus_tag="PKH58_13755"
     CDS             complement(2086..>2303)
                     /locus_tag="PKH58_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HNX90136.1"
                     /translation="SVDRETDKKKWEDIIINEKLSWQHYWDMNGKEANRLSIRAFPTN
                     FLIDNTGKIIAKNISVEELEVLLSKSL"
BASE COUNT          632 a          495 c          523 g          653 t
ORIGIN      
        1 tttcggtcgg gatactgctt acgggcagca ttcaccgttg ccttgagttt ggaaggcgaa
       61 tgtgcaaaat ccttgaaagc tgtttttccc ggtttgtcca ctattttctg catacggttg
      121 gacgctcccg ggaaatcggc aatggcttcg tagaactgat catccatgac acctatttga
      181 cggcaggcca gatgagcggc ttccatattt tgcaaattat gttctccgaa tatttttaac
      241 gcgatatctc cctttcgggt tttaacatag gttactccat cgcggatttc atatttaggc
      301 gtgttatatg gaaacgcgac tacatcacgc cgtgaatttt cagcttcgtt agccagcata
      361 ttcaggtttt catcaccatt ataatatatc agcctaccct gtgtttccat cgaatctata
      421 aacttgcgga actgatccac ataaatctca aaagtgggga aaacattgat gtgatcccag
      481 gcgatgccgg tgagtatggc aatatgaggt ttgtagaggt gaaacttcgg tcggcggtcg
      541 agggtggaag tgaggtactc atcaccttcg aacacggcaa tccgcgactc gaaggaaagt
      601 ttaaccatat tgtcgaaacc gtcgatctga gcaccgacca tataatcggc gtttatttta
      661 cattttttca gcacatagag tatcatggct gttgtagtag tctttccatg actgccacca
      721 accacgatgc gggttttgct gcgtgtctgc tgataaaggt attcgggaaa agaatagata
      781 tgaatgccga gttccctggc acggataagt tcaggattat cttccgtagc atgcattccc
      841 aggatgaccg cgttcagatt tttatggata cgttcgggga accagcccat ctcgcggggc
      901 agcagtccgt tttccttcag gcggctgtac gacggctcga aaatctcatc gtccgatccg
      961 gtcacttcat attcattttt tttactcagc gcgatggcca gattgtgcat ggccgctcca
     1021 ccgatggcta taaaatggac tcgttgcata ccgattattt ttgtaatttt gtcagattat
     1081 tgattgttac tgcaaatgta gtaagaaaat gcttaacgga gataacaaaa aaatgaaatt
     1141 gtacaaaatt gaagccggaa cttttcttgc cgacggcgga gccatgttcg gtgtggtgcc
     1201 caaaagggta tggcaaaaaa gatatccgtg cagcgatgaa aatttctgca tactggctat
     1261 gcgctgcctg ctggtggata ccggcgacag actgatactg gtggacagtg gaacaggtga
     1321 aaaacaactc gactacctga aatactatcg tttcaaaggc gtcgtcaatt ttgaaacgga
     1381 actgaacaag ctgggctatt catgcagcga tgtgaccgat gtgatactca cacacctgca
     1441 tttcgaccac tgcggaagct gtacccgttt taatgaagac cattcggaaa ttatactcac
     1501 tttccccaat gccacatact gggtgggaaa agcgcaatgg gagaacttcc tgaacccgaa
     1561 cgtgcgcgaa ggcgactcct atttcaaaga aaacatgctg ccggtgatgg aagccggaaa
     1621 actgagatta gtttctgaag acatgccgct cgatgataaa gtggaactca aattatatca
     1681 cggtcatact gcgggacaga ttgcgccctg ttttaatctt ggcgataaaa cactggtatt
     1741 cgccggtgac gtaatcccga ttatggcttc gttgcccgtt gcctgggttt cggcgtacga
     1801 caccttcccc attacttcca tggaagataa agtgaaaatg ctggatgaag cagcggagaa
     1861 acatcagata cttttctttg agcatgacaa ctacaacgaa tgctgcacgg tgaaaaaaat
     1921 caacggcaaa taccgggtgg agaaaatttt gactttggat gaagtgatga aatagtgaag
     1981 tgatatacta attcagactg taatcacagt tatatattag tcttcgaaac aacacacaac
     2041 ctgcaagtct atgcgagcag cggaggtttg ggaacttaga actggttata aacttttact
     2101 taagagtact tccaattcct ccacagagat atttttagca attatttttc ctgtattatc
     2161 aattagaaaa ttagttggga aagcacgaat ggataaccta ttcgcctctt ttccattcat
     2221 atcccaatag tgttgccaac taagtttttc gttaatgata atatcttccc atttcttttt
     2281 gtccgtttct ctatctacag ata
//
LOCUS       DAOPYB010000337         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_68497_length_2289_cov_8.931065, whole genome shotgun sequence.
ACCESSION   DAOPYB010000337 DAOPYB010000000
VERSION     DAOPYB010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68497_length_2289_cov_8.931065"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKH58_13760"
     CDS             complement(<1..404)
                     /locus_tag="PKH58_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNX90137.1"
                     /translation="MLNSPERIRTALIRWYNVNKRELPWRGITDPYKIWISEIILQQT
                     RVNQGYDYYLRFIGRFPDVVSLASADEDEVMKFWQGLGYYSRARNLHKAAKQIINTFG
                     GVFPSGYQDVLSLSGVGDYTAAAICSLAYHQP"
     gene            642..1754
                     /locus_tag="PKH58_13765"
     CDS             642..1754
                     /locus_tag="PKH58_13765"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX90138.1"
                     /translation="MKKSFLILWAAICLMACTQNTYNISGELSGSSRDSSYVYLTERV
                     NREWINLDSALIVDGKFELKGDADSVKVAYLRLQPKSGEESSDAFPFILEPGNITAHA
                     DSAEVFVITGTEQNNVLNKYTIEINALRAKSDDLYTRSGAGTGPLTADKKDSLDAGLK
                     VYADEMKNIEKEYALQNVNKMAGTHIFMTTFYDYTVEEKESLFALMNAKTKAIPRIAE
                     LIAATEVEKKTSKGQPYIDFSMTAPDGTTVKLSDYVGKTDYLLVDFWASWCPDCVASF
                     PDLTAFYARNKGTGFDIVGVSLDDEKDRWIRGIEKHKLNWHHMSDLQKWSSQGAKLYA
                     VNAIPATVLIDRKGNIAGRNMELKEIQDLLNQIVTK"
     gene            1891..2163
                     /locus_tag="PKH58_13770"
     CDS             1891..2163
                     /locus_tag="PKH58_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX90139.1"
                     /translation="MTKADVVNEIAKNTGIDKATVLATVESFMEVIKGSLAKKENVYL
                     RGFGSFVVKERAQKTARNISKNTTIIIPAHSIPSFKPAKTFVSNVK"
BASE COUNT          785 a          470 c          472 g          562 t
ORIGIN      
        1 taaggttggt ggtaagccag cgaacaaatg gcggcagccg tgtaatctcc aacgcccgat
       61 aaactcaata cgtcctgata accggatgga aatacacccc cgaaagtatt tataatttgt
      121 ttcgcggctt tatgcaggtt tcgggcacgg gagtagtaac ccagtccctg ccagaatttc
      181 atcacttcat cttcatcggc cgaagcgagc gacaccacat ccggaaaccg cccgataaac
      241 cgcaggtagt agtcatagcc ctgattaacc cgtgtctgtt gcaggataat ttccgatatc
      301 cagattttat acggatcggt aatcccccgc cagggcagtt cgcgcttgtt gacgttgtac
      361 cagcgtatca gtgctgtacg tattctttcg ggtgaattaa gcatcagatt tatgtataaa
      421 aagctgaatg actgataata aattgactgg aaaattgcat ataagataaa aaagcctcat
      481 ctttgcagca aagttactcc aaaactttcg aacccgaaat ttttactaca acacattata
      541 taactgacgc agttttgaat ttccataatt tacaaatatc aaaaggcttg ataatattat
      601 atggagattt catttgacca ataaaataca ataattatcg tatgaaaaaa tcatttttaa
      661 tcctttgggc agctatatgc cttatggcct gtactcagaa cacatacaac atcagtggcg
      721 aacttagtgg aagcagccgc gacagctcgt atgtgtattt aacggagcgg gttaaccgcg
      781 agtggataaa cctcgacagc gcactcattg ttgatggaaa atttgaactg aagggagatg
      841 ctgactctgt aaaagtggct tacctgagac ttcagccgaa atccggtgag gaaagttcgg
      901 atgcgtttcc gtttattctt gagccgggca atatcacagc ccacgccgat tcagcagagg
      961 ttttcgtgat taccggaacg gaacaaaaca atgtgctcaa taagtacacc atcgaaataa
     1021 acgctctccg cgccaaatcc gatgatcttt acacccgctc tggcgccgga acaggaccat
     1081 tgacagctga caaaaaagac tccctcgatg cgggactgaa agtttacgcg gacgaaatga
     1141 aaaatattga aaaggagtat gcgttgcaaa acgtcaataa aatggcggga acacatatat
     1201 ttatgactac cttctacgat tatacagtcg aagaaaaaga atcgctcttt gccctgatga
     1261 acgccaaaac caaagccatt ccccggatag ccgaactgat tgcagctacc gaagtggaaa
     1321 agaaaacctc caaaggtcag ccatacatcg atttcagcat gacggctccc gacggcacaa
     1381 cggtaaaact atcggactac gtgggcaaga ccgactatct gctcgttgat ttctgggcat
     1441 cgtggtgtcc cgactgtgtg gcatccttcc cggatctgac ggccttttac gccaggaaca
     1501 aaggtaccgg gtttgatatc gtcggtgttt cgctcgacga cgagaaggac agatggataa
     1561 gggggataga aaaacacaaa ctaaactggc atcacatgtc cgatttgcag aaatggtcca
     1621 gccagggagc aaaactttac gccgtgaatg ccattccggc aaccgtactc atagaccgta
     1681 agggaaatat tgccggacgt aatatggaat tgaaagaaat acaagattta cttaatcaaa
     1741 tagttacgaa ataattttta ggtaaatatt tcaaaaaaac agaactaatc cttttttaat
     1801 cagaagaaaa catctatatt tgcagtccgt tttcaaaaac agaaacagag agaaaatatt
     1861 atattaataa tttaaaaata aattacaaaa atgacaaaag cagacgttgt aaacgaaatt
     1921 gctaaaaata caggaattga caaagctact gtattggcaa ctgtagaatc tttcatggaa
     1981 gtgatcaaag gttcattagc aaaaaaagaa aacgtatatt tgagaggttt tggtagcttc
     2041 gttgtgaaag aaagagctca gaaaacagcg cgtaacatct ccaagaatac aactatcatc
     2101 ataccggcac attcgatccc ttctttcaaa ccggcaaaga cttttgttag caatgtaaaa
     2161 taagaatttc taacttttaa aaattaaaga aaatgccaag cggtaaaaaa agaaaaagac
     2221 ataaaatgtc tactcacaaa cggaaaaaaa gactgagaaa aaatagacat aaaaagaaat
     2281 agtctgttg
//
LOCUS       DAOPYB010000338         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_68855_length_2281_cov_7.531896, whole genome shotgun sequence.
ACCESSION   DAOPYB010000338 DAOPYB010000000
VERSION     DAOPYB010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68855_length_2281_cov_7.531896"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /locus_tag="PKH58_13775"
     CDS             <1..1714
                     /locus_tag="PKH58_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90140.1"
                     /translation="CGIYLNNCTGYSVQENKISGHYPSGISGNSYETGIVINNSGEAS
                     NEIYNNTFTRLQNGITAQDDNRGLVCKCNDYDSVKFDQSALVSGTPGSLGIAPMQGAS
                     DSVTSPAGNTFSPQHTTQQLPESDLKNQGAAFQYFHHRQAQSGVPPRVEPVYFSSSIS
                     RQNTGWDYDKFTACPSRLNGGGVENPIESSVEKLVQAQALIDSLETGLDELVDGGNTE
                     ETAANIVFSNPEEALDIYNDLLTKSPYLSDTVMTEAATKEEVISNTLLHDILVANPQS
                     ATSDEVLDQLDQRIDPMPEDMYNEILQGENILAPLTAEKASIAALKTDNTTLYYHLMN
                     RYLADTSQYATDSLLYLLDVQNTSEAAYLEAFIQLNNGEFTAMNSALSGISSEFDLSN
                     EEQLLHSYYQQFFALLQKMQTDTLPDLQADSLTTEQLVELVENSPEPVKSYSRNILVA
                     NHAIEYLEPYLYDDGLKSSSIRKKHRMGASTKQGSLLVFPNPAKDYIIVRYNLANELS
                     DAQEKAVLEIVKTTGQVIQSLTLQAVTGQVMVSTKTFVPGVYLIHIKGVSKKVEAIKF
                     NVIH"
     gene            1771..>2281
                     /locus_tag="PKH58_13780"
     CDS             1771..>2281
                     /locus_tag="PKH58_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90141.1"
                     /translation="MKIAFFFICIVVLQINTVVQEWVHYYGQGQNTVCRSITNDYDKG
                     MVISGMIDGYTYSWIIKTDINGEIIWNKRIGNGNYYCWGENIEKTSDGGKILCGTWQK
                     EDPEFDVFILKLNACGEIEWCKTLYTPGHYELCGKVKQTPEGDYILAANYFETNPFSR
                     TSLFKFNSSG"
BASE COUNT          744 a          436 c          467 g          634 t
ORIGIN      
        1 atgcggcatt tatctgaata attgtaccgg atactcggtc caggaaaata aaatatcggg
       61 ccattatcct tccggcatat caggtaacag ctacgaaacc ggtatcgtta tcaacaactc
      121 cggcgaagcc tccaacgaaa tttataataa tacttttacc aggttgcaaa acggtattac
      181 cgcccaggac gataaccggg ggctggtttg caagtgtaat gattatgata gtgtcaagtt
      241 cgaccaaagt gcattggtta gcggaacacc cggtagtctt ggaatagcgc cgatgcaagg
      301 tgcttcagac tctgtaactt cgccggcagg taatacattc tcacctcaac ataccaccca
      361 acaactccct gaaagtgatt tgaaaaacca gggcgcagct tttcagtatt ttcatcatag
      421 gcaggcacaa tcaggtgtac ctccaagagt cgaacctgtt tattttagtt cttccattag
      481 tcgtcagaat actggatggg attacgataa atttacagcc tgcccatcca gacttaacgg
      541 gggtggtgta gaaaatccta ttgaatcttc agtcgaaaaa ttagttcaag cgcaagcgct
      601 tattgattca ctcgaaaccg ggcttgacga attggttgat ggtggtaata cagaggaaac
      661 ggctgctaat attgtattca gcaatccgga ggaagctttg gatatttata acgatttatt
      721 aacaaaatcg ccctacctga gcgatacggt catgacggaa gcagcaacta aagaagaagt
      781 gatttcaaat acattacttc acgatatcct tgttgccaac ccgcaatcag ccacaagtga
      841 tgaggtattg gaccagttgg accaacggat tgacccaatg cctgaagata tgtacaacga
      901 gatattgcaa ggtgaaaaca ttctagcgcc tctgacagcc gaaaaggcct ctatcgctgc
      961 tttgaaaacc gacaatacta ctctgtacta ccatttaatg aacaggtact tagccgatac
     1021 atcacaatat gccaccgata gtctgttgta cctgcttgat gttcaaaaca cgtcagaagc
     1081 agcttatttg gaagcattca tacaactgaa taacggagaa tttacagcaa tgaattccgc
     1141 tttatccggt atctcttctg aatttgatct tagcaatgaa gaacaactat tgcatagtta
     1201 ttatcagcag ttttttgcac tattacagaa gatgcaaacc gacacacttc ctgatttaca
     1261 agccgatagc cttacaactg aacagttagt agaactggtg gaaaattccc cagagccagt
     1321 aaaatcatat tcccgtaaca tccttgttgc caaccatgca atagaatatc tcgaacctta
     1381 cctgtatgat gatggtttga aaagttcaag cattagaaag aagcaccgta tgggagcttc
     1441 aaccaaacag ggttcgctat tggtattccc taatcctgcc aaagattaca taattgtgag
     1501 gtacaacctt gcaaatgagt tatctgatgc tcaagaaaag gcagtattag aaatagttaa
     1561 aacaacaggg caggtgatcc aaagcctgac cttgcaagct gttacaggcc aagttatggt
     1621 ttcaacaaaa acttttgtac caggtgtgta tttgatacac attaaaggag taagcaaaaa
     1681 agttgaagct ataaaattta acgtaattca ttaattccta atacccatcc tgattaaaat
     1741 caggaggggt gtattgcaaa aatccctgtc atgaaaattg cttttttctt catttgcatt
     1801 gtagtacttc agattaatac tgtagttcag gaatgggttc attattatgg gcaggggcaa
     1861 aatactgttt gccgttctat taccaatgat tatgataaag ggatggtaat tagcgggatg
     1921 atagatggat atacttactc atggattata aaaactgata taaatggtga aatcatctgg
     1981 aataaaagaa taggtaatgg taactattac tgttggggag aaaatataga aaaaacatca
     2041 gatggaggta aaattctatg tggtacatgg caaaaggaag accctgaatt tgatgttttt
     2101 atcctgaagt tgaatgcatg tggagaaatt gaatggtgca agacacttta taccccaggt
     2161 cattatgaac tatgtggaaa agttaaacaa acgcctgaag gggattatat tttagctgcg
     2221 aattattttg aaaccaatcc tttttcaaga acatcacttt ttaaatttaa tagttcagga
     2281 g
//
LOCUS       DAOPYB010000339         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_68975_length_2279_cov_10.042266, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000339 DAOPYB010000000
VERSION     DAOPYB010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68975_length_2279_cov_10.042266"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PKH58_13785"
     CDS             complement(<1..718)
                     /locus_tag="PKH58_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90142.1"
                     /translation="MKKSAIFLILIIIGGLVSGQVINKAYINNGTGAYIVCGADFTND
                     AAGNVHNYGTIKLAGDFVNNQTFTSEPASYVMLNGAAQDIGGANSTIFNNLTIDGTGN
                     KTLSVNSSVANSLVMNANKILISNYNLSLYPAATIANSDNTRYVVTNGTGSLIKQSLP
                     LATDFTFPVGNSTSSYKPAILKIAGTSDDFAVRVLAGLNPTTGIDPYCVQNTWIISEG
                     TPGGTTAELNLGWNTPDQGGS"
     gene            complement(778..>2279)
                     /locus_tag="PKH58_13790"
     CDS             complement(778..>2279)
                     /locus_tag="PKH58_13790"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNX90143.1"
                     /translation="VTSGSGGHAFGLWNQVSGWDAIAIGDGVINSIDNTVQLGWNATP
                     AEGTMILTSGGNVGIGTTAPTYKLQVDNNALGNTQVKIGGINVWTAGHDNTLYFGDGG
                     YVYVGETYADDVLTLYGTQGMRIAPNGNYGTAGQVLTSDGTYASWANPSGGSGWALTG
                     NAGTSPSTNFVGTTDAQELTFRTNNAERMRITLRGQLVPKNTGYSIFMGESAGQVDDL
                     VSNYNVFLGYQSGMANTSGVDNIAIGTRALVRNTSATGNIAIGYWSCQNQSSGSANMN
                     TAVGYYSLYQNTGGYNTGVGYAAGAGVSSPNYTYCSFFGYDADFNVGGTYSNAMALGY
                     DATVTATNYVRVGNTSVTQIGGQVAWSTLSDGRVKENVKEDVKGLDFIMKLRPVTYNL
                     NKDKQDKLLNRFDKSDYPEKYDIEKIKFSGFIAQEVEQAATEVGYDFSGVNKPKHEND
                     LWGLKYSDFVVPMVKATQEQQKIIEAQQIKIDDLQKQIDELKALIKAKN"
BASE COUNT          586 a          519 c          449 g          725 t
ORIGIN      
        1 aagagcctcc ctggtcgggt gtattccagc ccaggtttaa ttcagcagta gtccccccgg
       61 gagtgccttc gcttattatc caggtattct gcacacagta aggatctatg cctgtagtgg
      121 gatttaagcc tgctaaaact cttacggcaa aatcatctga tgttccggca atcttaagaa
      181 tagccggctt ataactgctg gtgctgttac cgacaggaaa tgtaaaatca gttgccagtg
      241 gcagcgattg ttttattaaa ctccctgtac cgtttgtaac aacatatctt gtattgtccg
      301 aattcgctat cgtagcagcc gggtaaagtg aaagattata atttgatatt aaaatcttat
      361 tcgcattcat cacaagggaa ttggcaactg acgaattcac cgaaagggtc ttattacccg
      421 tgccgtcaat ggtcagattg ttgaatatgg tggaatttgc acctcctata tcctgtgcgg
      481 ctccgttaag catcacatag cttgcaggtt ctgaagtaaa ggtctggttg tttacaaagt
      541 caccagctaa tttgatggtg ccataattat gtacattccc ggctgcatca tttgtaaaat
      601 ccgcgccgca aacgatataa gcccctgtgc cattattgat gtaggctttg ttgattactt
      661 gcccggagac caggccgcct atgataatta agattaaaaa aattgctgat tttttcatga
      721 tctgtttatt ttatgctggg tttatattaa taccttatta tataaaaaac tatttttcta
      781 atttttagct ttaatcaatg cctttaattc atcaatttgc ttttgcagat catcaatctt
      841 aatttgttgt gcttcaatta ttttttgttg ttcctgtgtt gcttttacca tagggacaac
      901 aaaatcagag tatttcaggc cccaaaggtc attttcatgc tttggtttgt taacaccgct
      961 aaaatcgtaa ccaacttccg tggcggcttg ttcaacttcc tgagcaatga aaccactgaa
     1021 ttttattttt tcaatatcat atttctccgg gtaatcactc ttgtcgaacc tgttcaggag
     1081 tttgtcctgt ttgtctttat taagattata ggtaaccggg cgtaatttca tgataaaatc
     1141 caatccttta acatcttcct tgacattttc ttttaccctg ccgtctgata aggtcgacca
     1201 ggccacttgt ccgccgattt gggtcactga agtatttcca accctgacat aatttgtggc
     1261 ggtaacggtt gcatcgtagc ccagagccat cgcattgcta tatgttccac caacattaaa
     1321 gtcggcatcg tagccaaaaa acgagcaata ggtataatta gggctactta ctcctgctcc
     1381 tgcggcataa cctacaccgg tgttatatcc tcctgtattt tggtacaggg aataataacc
     1441 gactgctgta ttcatgttcg ccgaacctga tgattgattt tggcatgacc agtagccgat
     1501 agcgatattt ccggtagcac tagtgtttcg aaccaaagct cttgttccaa tagcaatgtt
     1561 gtcgactcct gaagtgtttg ccattccgct ttgataacca agaaatacat tgtaattcga
     1621 aacaaggtcg tcaacttgtc ctgcactttc acccataaat atggaatacc ccgtattttt
     1681 aggtactaat tgtcctctca aagttattct cattcgttct gcattattcg ttctgaaagt
     1741 taattcctgg gcatctgtgg ttcccacaaa gttagttgag gggcttgttc cggcgttgcc
     1801 agtcagtgcc cagccgcttc caccagatgg gttagcccat gaagcataag ttccatctga
     1861 agtgagcacc tggccggcag tgccataatt gccgttagga gctatcctca taccctgtgt
     1921 gccatacagc gttaatacat cgtcagcgta tgtttcacca acataaacat atccaccatc
     1981 gccaaaatac aaggtgttat catgtccggc agtccagacg ttgattccac caatttttac
     2041 ttgagtattg ccaagagcgt tgttgtcaac ctggagttta tatgttgggg cagtcgttcc
     2101 gatccccaca ttgcctccgc ttgttagaat cattgtgcct tcagccggtg ttgcattcca
     2161 gcccagttgc actgtattat caatagagtt gattacacca tctccgatag cgatagcatc
     2221 ccaacctgaa acttgattcc acagaccaaa agcatgaccg ccagaacctg atgttacag
//
LOCUS       DAOPYB010000340         2274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_69199_length_2274_cov_9.151420, whole genome shotgun sequence.
ACCESSION   DAOPYB010000340 DAOPYB010000000
VERSION     DAOPYB010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69199_length_2274_cov_9.151420"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1362
                     /gene="coaBC"
                     /locus_tag="PKH58_13795"
     CDS             151..1362
                     /gene="coaBC"
                     /locus_tag="PKH58_13795"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNX90144.1"
                     /translation="MLKGKKILLGVTGSIAAYKSAQIIRLLVKEGAEVKVIMTPLAKE
                     FITPLTLATLAKNPILVDFFDPTNGNWNSHVDLGLWTDLFLIAPATANTMAKMANGIA
                     DNLLLTSYLSARTPVFIAPAMDLDMFSHPATQKNINTLRQLGCTIIEPASGELASGLE
                     GKGRMEEPEKIVDCLRDYFTPKPLKDKIILITAGPTYENIDPVRFVGNYSTGKMGFAL
                     AEACAEAGAKVILIAGPVQLRASNPRIKRVHVTSADEMYAEAVKYFPEVNGAILSAAV
                     ADFKPVQQAEKKLKRGKDNMILELVPNPDIAQKLGEMKTADQFLVGFALETNDEETNA
                     LGKLERKNFDLIVLNSLMDKNAGFGYDTNKITILGRNGYKKQYELKSKPDVARDIVAE
                     INGLNINKTEE"
     gene            1359..2252
                     /locus_tag="PKH58_13800"
     CDS             1359..2252
                     /locus_tag="PKH58_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNX90145.1"
                     /translation="MKKYLLILTILSFCGLMEAQELKCNLVINSDKVQGSNKDVFKTL
                     QKEASEFMNNRRWTDLSFSEEERIDCNMNIIISAVEGDSYRAELTVQSRRPVYNSSYF
                     TSLINFKDNSFAFTYKEFEPLEFAENNLQSNLTSVLAYYAYLIIGYDMDSFSRLGGTP
                     YFQAAENIVTQAQSTDWAGWKAFENDKNRYALINNIMDEAFKKYRNYFYEYHRYGLDE
                     MTVNVTNGRAKILEGLPLLREANRARPSAVVISSFLDAKNDELINIFTEGTQKEKETV
                     VEILTDINPTSANRYEQIFKK"
BASE COUNT          706 a          451 c          503 g          614 t
ORIGIN      
        1 gttgttagct ggttttttgt atttttgtgg agccatcaac tttcacgaac tggtaactcc
       61 tggtttgcaa acagtaaacc actgaattaa tacagaaata catctgtttt tcaggccggc
      121 agttttttaa tattataaat agaacatttt atgttaaagg gtaaaaaaat attattgggt
      181 gtcacgggaa gtatagcagc atataagtca gctcagatta tacgtttgct ggtgaaagaa
      241 ggtgccgaag tgaaagtaat catgacacca ttggctaaag agtttatcac cccgcttacg
      301 ctggctacac ttgctaaaaa tcctattctt gttgattttt ttgacccgac taacggcaac
      361 tggaacagtc atgtcgattt ggggttgtgg accgatttgt ttctgattgc acctgcaaca
      421 gccaatacaa tggctaaaat ggcaaacggc atagccgata acctgctgct cacttcgtat
      481 ctgtctgccc gtacacctgt ttttatcgct cctgccatgg atcttgatat gttctcacat
      541 ccggcaacac aaaagaatat aaataccctt cgacagcttg gttgtaccat catagaaccg
      601 gcaagcgggg aactggcaag tgggctcgaa ggaaaaggac gcatggaaga accggagaaa
      661 atagttgatt gcctgagaga ttatttcaca cccaaaccgc tgaaagataa aataattctg
      721 attactgccg gtcctacata cgaaaacata gatcctgttc gttttgtagg taattattcg
      781 actgggaaaa tgggctttgc cctggctgaa gcctgcgcag aggcgggtgc aaaggtaata
      841 cttattgctg gtcccgtaca acttagagcc agtaatcccc gcatcaaacg cgtgcatgta
      901 acctcggccg atgaaatgta tgctgaagcc gtgaaatact tccccgaggt aaacggagcg
      961 atactaagcg ctgctgttgc cgacttcaaa ccggtgcaac aggcagaaaa gaaactcaaa
     1021 cggggcaagg acaatatgat tctggaattg gttccaaatc cggacatagc acagaaactg
     1081 ggtgaaatga aaactgctga tcagttttta gttggatttg cactcgaaac gaacgacgaa
     1141 gaaacgaatg cactgggtaa actggaacga aagaatttcg acctgattgt gcttaattcg
     1201 ctgatggata agaatgcggg tttcggttac gataccaaca aaattacaat tttaggtcgt
     1261 aacggctata aaaaacaata tgaactgaaa agcaaaccgg atgtggcgcg tgatattgtt
     1321 gcagaaatca atggtttgaa tataaataaa acagaagaat gaaaaaatat ctgttgatac
     1381 tgacaatatt aagtttctgt ggcttgatgg aggctcagga actcaaatgc aacctggtga
     1441 taaactcaga taaagtacag ggttctaata aggatgtttt caagacattg caaaaagaag
     1501 cttcggagtt tatgaacaac cgccgctgga ccgatttgtc gttcagtgaa gaagaacgga
     1561 ttgattgcaa catgaatatc ataatcagtg ccgtagaagg cgatagttac cgggctgaac
     1621 taaccgttca gtcgcgcaga ccggtatata attcttccta ttttacttct ttaatcaatt
     1681 tcaaggataa cagctttgct ttcacatata aagaatttga accgctcgaa tttgccgaaa
     1741 ataatctgca atcaaatctg acttcggtgc tggcttacta tgcttatctt attatcggtt
     1801 atgatatgga ttcattttcc cgccttggtg gaactcctta ttttcaggct gctgagaata
     1861 tcgttacaca ggctcagagc actgattggg ctggttggaa agcatttgaa aatgacaaaa
     1921 atcgctatgc gctgataaac aacataatgg atgaggcttt taagaaatat cgtaattatt
     1981 tttacgaata tcaccggtac gggctggatg aaatgaccgt taacgtgact aatggcaggg
     2041 caaaaatact ggaaggtttg cctttgctcc gtgaagctaa cagagcccgt ccctcggctg
     2101 ttgtcatcag ttcattcctc gatgccaaga atgacgaact tataaatatc tttacagaag
     2161 gaactcaaaa ggaaaaggaa actgtcgttg aaattctgac cgatataaac cccacttcgg
     2221 ctaaccggta tgagcagatt tttaaaaaat aaagcccccc tgccccctcc cgca
//
LOCUS       DAOPYB010000341         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_71755_length_2221_cov_12.357802, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000341 DAOPYB010000000
VERSION     DAOPYB010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71755_length_2221_cov_12.357802"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..>2221
                     /locus_tag="PKH58_13805"
     CDS             424..>2221
                     /locus_tag="PKH58_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90146.1"
                     /translation="MIKNYNFRKCNLRNVHILLLLITLLCTSGLYAQSTGDYRSNTTV
                     GNWTALTSWQRFNGTSWVTPTAAQGYPGQYAGTGAVTILSGHQINANFVSDPPPATIV
                     ITVNGVNNNNPFTAYTTPFGLGNVTVQSSAYLVLQGSGTGSTDYTIYLKTQELNINSG
                     GWLYFNGNKLNLGLPQNSVVEVCDGGLTGSSSDGNANQDIFIGDQWYAIGNSNKTQFT
                     FSEIMVNCLGTLESEPGIYSPVCVGQSIVLRGSYTGKVDTTPTYFWSIVSPSGTTTTY
                     TTQNVTISNPIVGTYLATLKVTTIRNGYTYTNSKTTSITVLPNASLALTSAPATTNQT
                     ICINSPLTNITYTAAGTSTVTFTGLPAGVIGNYSGNVATISGTPTVEGTFNYTVTASG
                     SCLPASQSGTFKVNQSYWVGTESNDWNTVANWTCGIPPTGADVVFAAITNSYGSMAVR
                     DLHLENTRTIGSLINASDKNLIIKPGSALVVNNTAPANDNTAKIQIQAASGQPNGSLI
                     LTNPQLNPNVYATVQMYSTGYKGATQWNWTDPDGKAYSGFYRWQYFGVPVESNATLVT
                     NTTLWGSYIRQYHENLELNKYYQKWSDISNNDR"
BASE COUNT          708 a          478 c          424 g          611 t
ORIGIN      
        1 tcaacgtgga gcaatttaat cccgatccgt tcatggaaca actcaacctg cagggtctac
       61 cgtggaaaga actacaggac gtagatctgg aactttaaca gatagaaaaa aaaacattca
      121 gcaaaacgta tgaatgtttt tttttgtctg gtaatctggt gattgttgat agatttttca
      181 acaattaaaa aattaaacaa ttataatgaa ataatttcat ccttaacttt ccagagtgtg
      241 aattcataaa atttgatgtt aaatctgata tttaagatat gaattcagta aaaattggct
      301 aatattgcat tatgacataa acctgattaa tagtatcgtt ctacaacaag tgtaagagta
      361 tctgtatttt cggccttttt gtaatttatc tgtctgtaat taacaatcta atattgaacg
      421 attatgatta aaaattacaa tttcagaaaa tgcaatctcc gcaatgttca tattttatta
      481 ctattgataa cccttctgtg cacctcggga ctttacgcac aaagtacagg tgactaccgc
      541 tcaaacacta ctgtcggaaa ctggacagca ctcaccagtt ggcaacggtt taatggcact
      601 tcatgggtta ctcccactgc cgctcaggga tatccggggc aatatgcagg aaccggagct
      661 gttaccattt tatcaggaca tcaaatcaat gccaattttg tatctgatcc tccgcctgca
      721 accattgtta taactgtgaa tggagtaaac aacaacaatc cattcaccgc atataccact
      781 ccttttgggc ttggaaatgt aaccgtacag agcagcgctt atttagtgtt gcaaggttcc
      841 ggaaccggga gcaccgatta taccatatat ctgaaaacac aggaattaaa tattaattca
      901 ggcggatggt tatattttaa cggaaataag ttgaatttag gattaccgca aaacagtgtt
      961 gttgaagtct gtgacggtgg acttacagga tcatcctccg atggtaatgc caatcaggat
     1021 atttttatag gtgatcagtg gtatgctatc ggaaacagta acaaaactca gtttacattc
     1081 agtgaaataa tggttaattg tctgggaact ctggaatctg aacccggtat ctattcaccc
     1141 gtgtgtgtag gtcaatccat tgtactcaga ggcagttata cagggaaggt tgatactacc
     1201 cctacctatt tctggtcaat tgtttcgccg agtggcacaa caacaaccta cacaactcaa
     1261 aatgtaacga tctctaaccc gatagttggt acttatttag ctactcttaa agtcactacc
     1321 atccgcaacg gttacacgta cactaacagc aaaacaacaa gtatcactgt attgcccaat
     1381 gcatcacttg cactcacttc ggcaccagcc actacaaacc aaacaatatg tatcaactca
     1441 ccgctgacaa atatcactta tacagcagca ggcacctcta cagtaacatt tactggtttg
     1501 ccggccggag ttatcggaaa ttattcgggt aatgtagcaa ctatcagcgg tacacccacc
     1561 gtagaaggaa catttaacta cactgtcacg gcttctggat cctgcttacc tgcgtcacaa
     1621 tcgggcacct ttaaggttaa tcagtcctac tgggtgggta cggagagtaa tgactggaat
     1681 acagtggcaa actggacctg tggcatacca cccaccggag ccgatgttgt ttttgctgca
     1741 ataacaaaca gctacggttc gatggctgtg cgcgatttac accttgaaaa tactagaaca
     1801 ataggaagtc ttatcaatgc cagcgataaa aatctgatta ttaaaccggg atcagcactt
     1861 gtagtaaaca atactgctcc ggccaatgac aatactgcta aaattcaaat acaggctgct
     1921 tcgggtcagc caaatggaag tttgatttta acaaatccac aattaaatcc gaatgtatat
     1981 gccacagtcc aaatgtactc taccggctac aaaggagcca ctcaatggaa ctggaccgac
     2041 cccgacggaa aagcctattc cggtttctat cgctggcaat atttcggcgt accggtagaa
     2101 agcaacgcaa cactcgtcac aaacactacg ttgtggggct cttatattcg tcaatatcat
     2161 gaaaatctgg aactcaacaa gtattaccaa aaatggagcg acataagtaa caatgatcgg
     2221 c
//
LOCUS       DAOPYB010000342         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_72031_length_2215_cov_9.351852, whole genome shotgun sequence.
ACCESSION   DAOPYB010000342 DAOPYB010000000
VERSION     DAOPYB010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72031_length_2215_cov_9.351852"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PKH58_13810"
     CDS             <1..1299
                     /locus_tag="PKH58_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HNX90147.1"
                     /translation="FGECNLAVDQNLLVMSIMKKVARRHGFRLLLHEKPFAGINGSGK
                     HNNWSLGTDTGVGLLTPGKTPAENLQFITFLVNVLMAVYKNNALLKASIMTAQNAHRL
                     GANEAPPAIISAFLGKQITAVLDKLENSTSDEAIVVSDKQQLSLGIAHIPEVLLDNTD
                     RNRTSPFAFTGNRFEFRAVGSSANCSSAMATLNAAVAAQLTEFKIAVDAKIAAGSTEQ
                     SAIFEILKEYIKISKPIRFDGNGYSEEWKVEAAKRGLDCETSVPEIYKAFTSEQSVKM
                     FTSTGVFTEKELEARNEVKWETYTKWIQIEARVLGDISMNHIIPLATKYQSFLLDNVC
                     KIKTVFSGEKAEKLSTLDTALIVEIGERIATIKNAVDDMIDIRRSVNKIEDEYEKAVA
                     YHDKVLPYFDIIRYNADKLELIVNDEMWTLPKYREMLFIR"
     gene            1453..>2215
                     /locus_tag="PKH58_13815"
     CDS             1453..>2215
                     /locus_tag="PKH58_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HNX90148.1"
                     /translation="MSLSPLQIDEYEQHSLYSFQNEHDACGVGLVINLSGDKSHEIVD
                     NGLQVLENMVHRGAESADNKTGDGAGILVHIPHEFILLQGIAVPSKGKYGTGLVFLPK
                     DKKKSGDCLNVLKDYIEKEGLTLLAVRDVPVNSQILGEISASNEPDIKQIFVTGNLQQ
                     DELETKLYIVRKKIEKAIQQSKLAAERSFYIVSLSTKQMIYKGMLTSLQLREYYPDLS
                     NPNFTSRIALVHSRFSTNTFPTWDLAQPFRMIGHNG"
BASE COUNT          690 a          479 c          484 g          562 t
ORIGIN      
        1 tttggagaat gtaacctggc tgtcgaccaa aatctgctgg tgatgtccat tatgaaaaaa
       61 gtggcacgtc gtcacggttt tcgtctgctg ctgcatgaaa aaccctttgc tggcattaac
      121 ggctccggga aacacaataa ctggtcactc ggaactgata caggcgtcgg attgcttaca
      181 ccgggaaaaa ctccggcaga aaatcttcaa tttatcacct ttttggttaa cgtcctaatg
      241 gccgtatata aaaataatgc cttgctgaaa gcatccatta tgacggctca gaatgcgcac
      301 cggttaggtg caaatgaagc accgccagcc atcatttcag cgtttctcgg aaaacagatt
      361 acagccgtgc ttgataaact ggaaaacagc accagcgatg aagcaattgt agtgagcgac
      421 aaacaacaac tgagtttggg tattgctcac attccggaag tattgctcga taataccgac
      481 cgcaaccgta cttcaccgtt tgcttttacc ggtaatcgtt ttgaattccg agcagtgggt
      541 tcatccgcca actgttcctc agcaatggca actctgaatg ctgccgtagc cgcccagctg
      601 actgaattta agatagctgt ggatgctaaa atcgctgccg gctccaccga acagagtgcc
      661 atatttgaaa ttctgaaaga atatatcaaa atctcaaaac caatacgttt tgacggcaat
      721 ggttatagcg aagagtggaa agtcgaagca gcgaaacgcg gactggattg cgaaacaagc
      781 gttcctgaaa tttacaaggc atttacgagc gaacaaagcg tgaaaatgtt cacctcaaca
      841 ggtgtattca ccgaaaagga acttgaagcc cgcaacgaag tgaaatggga aacttacacc
      901 aagtggatac agattgaagc gcgggtgttg ggagacattt ccatgaacca catcattccg
      961 ctggctacta agtatcagag cttcctgctt gataatgtgt gtaaaataaa aactgtgttc
     1021 agcggtgaaa aagcagagaa actcagcaca ctcgacacag ctcttatcgt tgaaataggt
     1081 gaacgtattg ctacaatcaa aaatgcagtg gacgatatga ttgatattcg ccgctcggtc
     1141 aataagattg aagatgaata tgaaaaagcg gttgcatacc atgataaggt attaccttat
     1201 ttcgatatta tccgctacaa cgctgacaaa ctggaactga ttgtgaatga tgaaatgtgg
     1261 acattgccta aatacaggga aatgttgttt atcagataga tgatcgaatt tctatgactt
     1321 tctcaaataa aaaatagtga gagtttgtat tcaacaagac ttctcactat tttttagaat
     1381 aaaacttaaa acaaaaccga ttgcaagcga gattgtttct ccggaagcag cctccctatt
     1441 tcccaactaa ttatgagttt aagcccatta caaatagatg aatatgagca gcattcgctg
     1501 tattcattcc aaaacgaaca cgatgcctgc ggtgtaggcc tggttattaa tcttagcggt
     1561 gacaaatctc acgaaattgt ggataatggt ctgcaggtac ttgaaaacat ggtacaccgc
     1621 ggagccgaaa gcgccgacaa caaaaccggt gacggagcag gaattttagt gcatattccg
     1681 cacgaattta ttcttttaca gggcattgcc gttccttcga aagggaaata cggtaccgga
     1741 ttggtttttc tgcccaagga taagaaaaaa tcgggcgact gtctgaatgt gctcaaagat
     1801 tatatcgaaa aagaaggatt gacgctgctt gcagtacgtg atgtaccggt caacagccaa
     1861 attctgggtg aaatttccgc ttccaacgaa cccgatatta agcagatatt cgttactggc
     1921 aacctgcaac aggatgagct ggaaaccaaa ctttacattg tccgtaaaaa aatagaaaaa
     1981 gccattcagc agtccaaact ggcagctgaa cggagttttt atattgtcag cctttccacc
     2041 aaacaaatga tttacaaggg aatgctcact tcgctgcaat tgcgtgaata ctatcccgac
     2101 ctgagcaatc ctaattttac aagccgcatt gcattggttc actcacgttt cagcaccaac
     2161 acattcccga cctgggatct ggctcaaccg ttccgtatga tcggccacaa cggtg
//
LOCUS       DAOPYB010000343         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_72136_length_2213_cov_10.500000, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000343 DAOPYB010000000
VERSION     DAOPYB010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72136_length_2213_cov_10.500000"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKH58_13820"
     CDS             complement(<1..415)
                     /locus_tag="PKH58_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90149.1"
                     /translation="MRKITVLFLSVLVLTACTKTALNLPADSNNNINLNTLLTLKSGV
                     NKVLTQDYIMNIDEIDSVSTPGIDAALSADKSTITLNITPETPAFFNTTIWVKGVPYA
                     VPCRKTDKVDYVFTYDPKGKTYKKVQIAGQMNDWAP"
     gene            complement(442..2163)
                     /locus_tag="PKH58_13825"
     CDS             complement(442..2163)
                     /locus_tag="PKH58_13825"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HNX90150.1"
                     /translation="MAFPKFSFKNFGEKLQQALMRFPITIAAILTFAVLMIIQIHNNF
                     KDLFDERLWWMLGLTILLSLAFYLFAENRIGCLLKNILNVLLIACVAWFTFTLQKPVV
                     EAEIIRMLVLGVSFGAAVFFAPFITGKRPLAFWNYVLDVIYQLITAYVFAGVLMAGLS
                     LALLSLDKLFGMNIPDNSYGYLSVLCFVLFGSVYFLSSMPRLSISEEEKTVEFPKIYK
                     ILGLYILLPILGIYILILYVYLAKIIVTWELPNGWVSWLVSILGIVGYLTISLIHPIY
                     LKGENKPAKLFTRFFPIILLPLLILMFVGIMRRLSDYGITINRLYVLLLNLWLFGVSI
                     YIFLTKTRQVKWIYISFAAIAFLFSVGPWSVFSITKHTLKSELTRLYTEAKPFISDQK
                     NPEIKSLDTAKKKRLYDITRYLKQTYGKESVKTVIVSVFGKDVKTDNLISVPAIYEDE
                     KTEKYFSLYSREESIELPITGYNTFIKLSKTYNDNYLYKNDSLSVMVKNSQIEIIQQD
                     KVLTVISLEKLIKNSFENDRNEIKPEDAALSGTNYKLLVFSLEGKRKAENKEVKITNM
                     NAIILLK"
BASE COUNT          687 a          437 c          436 g          653 t
ORIGIN      
        1 ccggagccca gtcgttcatc tgaccggcaa tctgcacttt tttataggtt ttcccttttg
       61 gatcataggt gaatacatag tccaccttat ccgttttgcg gcaaggaact gcgtaaggca
      121 caccttttac ccagatggtg gtgttgaaaa aagccggagt ttcgggtgta atgttcagtg
      181 taatggtaga tttgtcggca gacaaggcgg catcaattcc gggagtgctt acgctgtcaa
      241 tctcatcaat attcatgata taatcctgtg tcaatacttt attcacaccg gatttcagcg
      301 taagtagtgt gttcaggttg atgttattat tactgtcggc aggaagattg agcgctgttt
      361 tagtgcaggc ggtcaacacc agtacagaca gaaaaagaac ggttattttt ctcatacgtt
      421 ttataattgt gaattaatac tttatttcag gagaataatg gcattcatat tagttatttt
      481 cacctcctta ttttcagctt tgcgctttcc ttccagactg aaaaccaaca gtttgtaatt
      541 tgtgcctgac aatgctgcat cttccggctt aatttcgttt ctatcgtttt caaatgaatt
      601 ctttatcagt ttttcaaggg atataactgt caagacctta tcctgttgaa taatttcaat
      661 ctgtgaattt ttaaccataa ccgataaact gtcatttttg tataaataat tgtcgttgta
      721 tgtttttgac aattttataa atgtgttgta accggttata ggcaattcaa tgctttcctc
      781 ccgggaatac agagaaaaat atttctcggt cttttcatct tcgtaaatag ccggaacact
      841 gattaaatta tccgttttga catctttacc aaatacagaa acaatcaccg ttttgacact
      901 ttctttcccg taagtttgct tcaaatagcg ggtaatatca tacagccgtt ttttctttgc
      961 ggtgtcgagg cttttaattt caggattttt ttgatcactg atgaaaggtt ttgcttcagt
     1021 gtacaagcgg gtgagttcac ttttcaatgt gtgtttcgtg atgctgaaca cactccacgg
     1081 acccactgaa aaaagaaacg ctatcgctgc aaatgaaatg taaatccatt tcacctgtct
     1141 ggttttggta aggaaaatat aaatactgac gccgaaaagc cagagattga gcagtaaaac
     1201 atacaaccgg ttgatggtga taccgtagtc cgaaagccgg cgcatgatgc ccacaaacat
     1261 caaaataagc aaaggcaaaa gaataatcgg gaaaaatcgt gtgaatagtt ttgccggttt
     1321 attttcacct ttcagataaa ttggatgtat caagctaata gtcaggtaac cgacaatgcc
     1381 cagtatggaa accagccacg aaacccaacc attcggcagt tcccaggtta caataatttt
     1441 agccagataa acatataata tcaggatgta aatacccaga atgggcaata aaatatataa
     1501 gccaagtatc ttgtaaattt taggaaactc taccgttttt tcttcttcgc ttatactcaa
     1561 acggggcatt gacgaaagaa aatatacgga gccaaaaagc acgaaacaca atacagaaag
     1621 gtagccgtac gagttatccg gaatattcat accgaaaagt ttatcgagcg aaagcagtgc
     1681 caggctcaaa ccggccatca gaactccggc aaaaacatag gctgtaatca gttgatagat
     1741 aacatccagt acgtaattcc aaaatgcaag cggacgtttg ccggtaataa aaggggcaaa
     1801 aaacacagcc gcgccgaaac tgacacccag caccaacatc cggatgattt cggcttccac
     1861 caccggtttt tgtaaggtga atgtaaacca tgcaacacaa gcaatcaata gtacattcag
     1921 aatatttttc agcagacagc caattctgtt ctcggcaaaa agataaaatg ccagcgaaag
     1981 taaaatggtg agccccagca tccaccaaag ccgttcatca aaaaggtctt tgaaattgtt
     2041 gtggatttga ataatcatca ggactgcaaa ggtgagaata gctgcaatcg taatagggaa
     2101 acgcatcaaa gcctgctgca atttctctcc gaaatttttg aatgagaatt ttgggaatgc
     2161 catgttgact gattttaatg taattcaagt atcaaaactg tttttccggg aat
//
LOCUS       DAOPYB010000344         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_72530_length_2205_cov_7.014884, whole genome shotgun sequence.
ACCESSION   DAOPYB010000344 DAOPYB010000000
VERSION     DAOPYB010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72530_length_2205_cov_7.014884"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1215)
                     /locus_tag="PKH58_13830"
     CDS             complement(115..1215)
                     /locus_tag="PKH58_13830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90151.1"
                     /translation="MNLIWEKSIESTALKVQNARMTLFDGSTLVAVFTLKLSNAFDFC
                     QYIVKIDYNGNIIQSSIFGPNHGVIFDILKLGYDSKLLLPAMGFGEINSTGQIIVLDS
                     SLNQVYIDSIPGNVSNCCSVKSLNDSVYFLAGNKSYPFNEESYNIAFMKMNFPNIVSG
                     SHTVGRIADTVDFGGSFKSMDFSDPSNIYVAGTSNLSVSGGYYAYQPSWYLISRFDSA
                     LNIYWTKYYGGDAYYVLATVTATSDGGAIVAGSRFDYLNHPENQLDVYVLKVDSTGLY
                     VNVDEEKPIPIHDAIVYPNPGSDYLIVQSGPQVSGAVFRMYDMQGRQVMEERLTSTLL
                     HLSAGNLSAGTYPWQIIFNNKTIENGKWVKDK"
     gene            complement(1653..1973)
                     /locus_tag="PKH58_13835"
     CDS             complement(1653..1973)
                     /locus_tag="PKH58_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90152.1"
                     /translation="MSKQCFRLASEKKMRANQEMFWSSSQYPNHHGCLSPEECNFYLA
                     GTKWIIYTAESTGGERPDEATFISVDVEGNEYLWPDISDYFHEMYIEYGILHISPNPP
                     NPID"
     gene            complement(1976..>2205)
                     /locus_tag="PKH58_13840"
     CDS             complement(1976..>2205)
                     /locus_tag="PKH58_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90153.1"
                     /translation="VYPNPGSDYLIVQSGPQVSGAVFRMYNMQGRQVMEERLTSTLLR
                     LNAGSLAAGPHPWQIIFNNNVIENGKWIKDK"
BASE COUNT          651 a          430 c          449 g          675 t
ORIGIN      
        1 agtagttacc gccgggggct gttctgcaaa tcataatctt gttctgcatt tgttctgatt
       61 gtggttggcg catctttctt tcagatgtgt gccggaaata ttgttttgac atatttattt
      121 gtctttcacc catttcccgt tttcaatcgt tttgttgttg aaaataattt gccaggggta
      181 agtgccggca gagaggttac cggcgctaag gtgcagcagg gttgaggtaa gccgttcttc
      241 catcacctgc cggccttgca tgtcgtacat ccgaaaaaca gctccgctta cctgcggccc
      301 cgattgtacg atgaggtaat cgctgccggg attgggatat acaatggcat catgaatggg
      361 aatgggtttt tcttcgtcaa cattaacata caatccggtt gaatctactt tgagcacata
      421 aacatcgagc tggttctcgg ggtggttgag ataatcgaaa cgagaaccgg caacaattgc
      481 acctccgtca gatgtggcag tgacagttgc cagaacataa taggcgtctc caccgtaata
      541 cttggtccaa tagatgttta aagccgaatc aaatctgctg ataagatacc aggaaggctg
      601 ataagcgtaa tatcctccag aaaccgaaag atttgatgtt cctgccacgt aaatattcga
      661 tggatcacta aaatccatac ttttaaatga accgccaaaa tcaacagtgt ctgcgatgcg
      721 tccaacagta tgtgaacctg aaacgatgtt tgggaagttc atcttcataa atgcaatatt
      781 gtaactttcc tcgttaaatg gatagctttt attacctgcc aggaaataga cggaatcgtt
      841 taaagatttt accgaacagc aatttgatac atttcctgga atagaatcaa tatatacctg
      901 attcaatgaa ctgtcgagta caataatttg tccagttgaa tttatttcac caaaacccat
      961 agctgggaga agaagttttg agtcataacc taattttaaa atatcaaaaa taacgccatg
     1021 gttaggtcca aaaattgagg attgaataat gttaccatta taatctattt ttacaatata
     1081 ttgacaaaag tcaaatgcat tgcttagttt taatgtaaaa acagcaacta aggtacttcc
     1141 atcgaaaaga gtcattcttg cattctgaac ttttaatgct gttgattcaa tgcttttctc
     1201 ccagattaaa ttcaatgagg tgtctataca cgcaatccat atacttgctg gtgaattctt
     1261 gttcaagtgg cctccgacgc aaatgatatt attactcgag atttgataca tatcattaaa
     1321 aaaacatgaa gtgtctttat tgataattaa ggattgcaaa gagtcaccat aagaattaaa
     1381 tttaaatatt ttttcccaat atttcgtatc ttgatttgtc ctttgaatgg tactaaggat
     1441 aaaccttgaa ggagcatatt caattatgcc gcctgataca ttttcgaatt ttagtgattc
     1501 gagtaaaagt gaaaatgatg attgcccgta caaatagcaa tggcattggg ttatcaaaat
     1561 tacaacaaga aatttcttca tctgagttaa ataattaata gtatgaagac cacatcagga
     1621 tcaacaaatc ctgatgtggc cagaaatttg aatcagtcta tcggatttgg cggatttggc
     1681 gatatatgca agatcccata ctcaatatac atttcatgaa aataatcgga aatgtcaggc
     1741 cataaatatt cgttaccttc tacatcaaca gagataaaag tggcttcatc aggcctttca
     1801 ccgccggtgc tttcggctgt gtaaatgatc catttagtcc ctgcaagata aaagttgcat
     1861 tcctcggggc ttaaacaacc gtggtgatta ggatattgac tggaactcca gaacatctct
     1921 tggttggcac gcatcttttt ttcagatgcg agccgaaaac attgttttga catatttatt
     1981 tgtctttgat ccatttcccg ttttcaatta cattgttgtt gaaaatgatt tgccaggggt
     2041 ggggtcctgc ggcgaggctg cctgcgttaa ggcgcagcag ggttgaggta agccgttctt
     2101 ccatcacctg ccggccctgc atattgtaca tcctgaaaac agccccgctc acctgcggcc
     2161 ccgattgtac gatgaggtaa tcgctgccgg ggttgggata tacaa
//
LOCUS       DAOPYB010000345         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_73102_length_2194_cov_7.322581, whole genome shotgun sequence.
ACCESSION   DAOPYB010000345 DAOPYB010000000
VERSION     DAOPYB010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73102_length_2194_cov_7.322581"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..417)
                     /locus_tag="PKH58_13845"
     CDS             complement(202..417)
                     /locus_tag="PKH58_13845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90154.1"
                     /translation="MYFLTHYFYRTTVISITDLTGKEIRKIEKGIKDKGTYNVDFVNS
                     NLNAGIYFYSLILDGKKSDTKKMVITR"
     gene            471..1670
                     /locus_tag="PKH58_13850"
     CDS             471..1670
                     /locus_tag="PKH58_13850"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNX90155.1"
                     /translation="MDKATHFFGTSVFGQLISLIQPTIINQAVNAYNSDRYVKKFRTK
                     DHLISMLFCAFAKCTSLREVSGAMLGLSGKTRHFQLEHIPFRSTLGDANQFRNAEVFG
                     FIYHKLLKKYSGFISDSRIKNVINKQIEIFDSTTISLFKDIMKCVGRKPKNGKRKGGL
                     KVHTVINVDETVPKMVWFTHAATNDHILLNKLKMDSNTIYVFDKGYNDYKAFEKFCTH
                     QTGFVTRIKDNAVYQCVEKQKIEEYIHSGVIEDEIITLTVKDGDESRKLTLRKIRFYD
                     REFKREYEFLTNLFDIRADLVAAIYKLRWQIETLFKQFKQNFPLKYFLGDNENAIKIQ
                     IYCALIANLLLTVIQKQLKHRWAFSNLVSFCKIHLFNYIHLMHFLENPNKDWRKALCN
                     EEQLALF"
     gene            complement(1808..>2194)
                     /locus_tag="PKH58_13855"
     CDS             complement(1808..>2194)
                     /locus_tag="PKH58_13855"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90156.1"
                     /translation="QVIKIAAQNQCGTNWNQPFQKTFYAINCFGDNSFTLSPNPASNE
                     VVIKTNDLSTIYSENRHENSDESSTFTEVRIFDQFGNQKQNNKYVIGTKETVINTSSL
                     MTGTYFVQILHGTKKIWEQYTLIITK"
BASE COUNT          670 a          397 c          415 g          712 t
ORIGIN      
        1 atactatttg tatagtatac aagaatattc tttggcggtt tttctctgca ttgttaacgg
       61 ctgaagctaa gtgcaggcgg gcatttcaaa gctattaata ttcaataaac tacaatgttt
      121 agtaggcgta atttgcttca aatcagcact tttagcccgc ttgcacttag cttttgttgg
      181 ctgctgtatg tcttttccgt cctatctcgt tattaccatt tttttagtat cgctcttctt
      241 tccatccagt atgagtgaat agaagtatat gccggcattg aggtttgaat tcacaaagtc
      301 aacattgtag gttcctttgt cctttattcc tttttcaatc ttccggattt cttttccagt
      361 cagatcagta attgaaatca ctgttgtccg gtaaaaataa tgagttagga agtacatgat
      421 gttcaaccca actcattttt gtagttttat gtcgccaaac aacaaaccac atggacaaag
      481 ctacacattt tttcgggaca tcggttttcg gacagctgat ttcgcttatt caaccgacca
      541 tcattaacca agctgtcaat gcatacaatt ctgatcggta tgtcaaaaag ttcaggacaa
      601 aggatcacct gataagtatg cttttctgtg catttgctaa gtgcacatca ctgagggagg
      661 tttctggagc aatgctggga ttatcaggca aaacccgaca ttttcaattg gagcacatac
      721 ccttcagaag cacccttggt gatgcaaatc aatttcgtaa tgctgaagta ttcggcttta
      781 tctatcataa actgctaaaa aaatatagtg gctttatttc ggacagccgg attaagaatg
      841 tgataaacaa acaaatagaa atatttgaca gcacaactat cagtcttttt aaagatatta
      901 tgaagtgcgt tggccgaaag ccaaagaatg gcaagcgcaa aggaggtcta aaagtgcata
      961 ccgtcattaa tgtggatgaa accgtgccaa aaatggtctg gtttactcac gccgctacaa
     1021 acgatcatat tctactcaac aagctgaaga tggactcaaa cactatctat gtattcgaca
     1081 aagggtacaa tgattacaaa gcctttgaga aattctgtac ccatcaaacc ggttttgtga
     1141 cgcgtatcaa ggataatgcg gtttatcaat gtgttgaaaa acagaaaatt gaggagtata
     1201 ttcattcggg tgttatagaa gatgagataa ttaccctaac ggtgaaagat ggggatgaaa
     1261 gcagaaaact cactctgaga aagatccgat tctatgacag ggaatttaag cgggaatatg
     1321 aattcctcac caacctgttc gatattcggg cggacttggt ggctgctatt tataaactcc
     1381 gctggcaaat agaaactttg ttcaaacagt ttaaacaaaa cttcccgtta aaatacttcc
     1441 tgggtgataa cgaaaatgcc atcaagattc agatttactg cgcactgatc gctaaccttt
     1501 tattaactgt tatacaaaaa caactcaaac accggtgggc attctcaaac ctggtaagtt
     1561 tctgcaaaat tcacctgttc aattacattc acctgatgca tttcctcgaa aacccgaaca
     1621 aagactggcg aaaagcacta tgtaatgaag agcaattggc tctgttctaa agtggcttac
     1681 ttcgaaaaat gtgtaaaatc aaattgaatt cacattgatt gtcaggtatt tagttagtta
     1741 tgagtaacaa tttttaactt taccggacaa cagtgaattc aaacaataat ctggatttaa
     1801 ttatgaatta ttttgtaatt attagggtat attgttccca gattttcttt gttccatgta
     1861 atatttgtac aaaatatgtt ccggtcatta gtgaagaagt attgattact gtttcttttg
     1921 tgccaattac gtatttgtta ttttgttttt gattgccaaa ctggtcaaaa attcttactt
     1981 cggtaaaagt tgatgactca tcgctattct catgtctgtt ttctgagtaa atagttgata
     2041 gatcatttgt ttttatcact acctcattgg aagccggatt tggtgaaagg gtaaatgaat
     2101 tatccccaaa gcaatttata gcataaaagg ttttttggaa cggttggttc cagtttgtgc
     2161 cgcattggtt ttgtgctgca attttaatta cctg
//
LOCUS       DAOPYB010000346         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_73152_length_2193_cov_9.183349, whole genome shotgun sequence.
ACCESSION   DAOPYB010000346 DAOPYB010000000
VERSION     DAOPYB010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73152_length_2193_cov_9.183349"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1423)
                     /locus_tag="PKH58_13860"
     CDS             complement(131..1423)
                     /locus_tag="PKH58_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNX90157.1"
                     /translation="MFEKYFQIKGSGSNMRTEILAGITTFLTMAYILAVNPNILSATG
                     MDKGALFTATAVASIVGTLAMSLLAKLPFAQAPGMGMNAFFAFTVCLGMGYSWEFALT
                     AILCEGLIFLVLNFFNLRAAIVDAIPATLKNAISAGIGLFIAFVGLQSSGIVVKNDAT
                     LVTIGQITSGTALLALIGIIITSVLLIKKVKGGLLIGIIATAIIGIPMGITKFTGIMD
                     VPPSPAPLFFKFDFSKIFTWDMLIVVFTFLFVDVFDTVGTLIGVCSKANMLDAKGQIP
                     NVKKAFYSDAIGTLSGAVFGTSAVTTYVESASGVSEGGRTGMTSLVTAACFALALLFA
                     PLFLSIPGAATAPILILVGLMMLDPIKDIDLTDYSEAIPAFMCIIAMPLMYSIAEGIA
                     LGLLAYVFLNVISGKFKKVSIAMYILAVLFILNYIFIQ"
     gene            complement(1465..2163)
                     /locus_tag="PKH58_13865"
     CDS             complement(1465..2163)
                     /locus_tag="PKH58_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HNX90158.1"
                     /translation="MRKVLFALLLLSTVSVAQAQNLQIHYDFGENRKHVTTTFEMFKP
                     DKWGNTFAFIDYDFNYGSKNSPSGSYMEIARCLNFWGGPLSAQIEYNGGLGGYATTSD
                     YTGGSYAINNAWLLGVDYFMHSKDFSRTLNLKALAKNIVGKNYTPQFTGVWTIQMLSK
                     KLTFTGFADLWWEKGTYANAGADANPVFITEPQLWYNFTPNISAGSEVEVSTNFAGNE
                     GFMVNPTLAVKWNF"
BASE COUNT          687 a          445 c          446 g          615 t
ORIGIN      
        1 ttagtcattt tgatttaatg attggttaat tgattaattg gaaaaacggc ttcgaaatac
       61 atcgaagccg tttttatatt aagtttattt ttattggata attctttgca atacgatatc
      121 gaaagaagtt ttactggata aaaatataat tcaaaataaa cagcacagct agtatataca
      181 tggcaatgga tacctttttg aatttgccgc tgattacatt cagaaatacg tatgcaagaa
      241 gtcccagagc aataccttcg gcgatactgt acattaatgg catggcaatg atacacataa
      301 acgcgggaat agcctcggag taatcggtca aatcaatgtc cttgatagga tctagcatca
      361 tcagaccaac caggattaga attggagcgg tagcagcacc cggaatggaa agaaacagtg
      421 gtgcaaacaa gagcgctaac gcgaagcaag cggctgtaac cagcgaagtc atacctgtac
      481 gtccaccttc ggatacacca gaagcacttt caacataagt tgtaacggca cttgtgccaa
      541 atacagctcc cgaaagtgtt ccgatagcat ctgagtaaaa tgcttttttt acattcggta
      601 tctgaccttt tgcatccagc atattagcct tcgaacaaac gccgataagc gtacctacgg
      661 tgtcgaacac atcgacaaaa aggaatgtaa ataccacgat cagcatatcc caggtgaaaa
      721 tttttgagaa gtcaaatttg aagaacaggg gagctggact gggcggtaca tccattattc
      781 cggtgaactt ggtgataccc atcggaatac cgataatggc agtggctatg ataccaatca
      841 gcaagccgcc tttcactttc ttgatcagaa gaactgaggt gataattatg ccgataagag
      901 caagcagggc agtgcctgag gttatctgac caatggttac aagagtagca tcgtttttaa
      961 caacgatacc tgaactttgt aaacctacaa aggcgataaa taaaccaata ccggcactga
     1021 ttgcgttttt gagagtagcc ggtatggcat cgacgatagc tgcacgcaga ttgaaaaagt
     1081 tcagtaccag aaaaatcaag ccttcgcaaa gaattgctgt tagtgcaaat tcccacgaat
     1141 aacccatgcc aagacaaact gtgaatgcaa agaaagcatt cattcccata ccgggtgctt
     1201 gtgcaaaagg taacttagcc agtaaagaca tggcaagtgt tccaacaatg gatgctactg
     1261 cggttgccgt aaacagagca cctttatcca ttcccgtagc tgaaagaatg ttaggattta
     1321 cagccagaat ataggccatc gttaaaaaag tggtaatacc ggcaagaatt tccgttctca
     1381 tattcgagcc acttcccttt atctgaaaat atttttcaaa catatttttg atatattaat
     1441 ggtaaatctt ttgtttctct gaatttagaa attccatttt acagccagcg tgggattaac
     1501 cataaatcct tcgttaccag caaaattggt agatacttca acttcgcttc ctgcagagat
     1561 attaggcgtg aaattatacc aaagctgagg ttcggtgatg aataccggat tggcatcggc
     1621 acctgcattg gcatatgttc ctttttccca ccataaatca gcaaaaccgg taaaagttaa
     1681 ttttttgctg agcatctgaa tagtccaaac accggtgaat tgcggggtgt agtttttccc
     1741 tacgatgttt ttggctaatg ctttcagatt caatgttctc gagaaatctt tgctgtgcat
     1801 aaaataatca acacccaaca accaggcgtt gttgattgca tacgatccgc cggtgtagtc
     1861 ggaagtggtg gcatatccac caagaccgcc gttatactct atctgggcgc tcagagggcc
     1921 tccccagaag ttcagacaac gggcaatttc catgtagctg cccgatggag agttcttgct
     1981 tccatagtta aaatcataat cgataaatgc aaatgtattt ccccatttgt cgggtttaaa
     2041 catttcaaaa gttgttgtta catgctttct gttttctcca aaatcgtaat gaatttgaag
     2101 attctgtgcc tgagcaactg atacagtact taataataac agtgcgaata gtacttttct
     2161 cataacctga tgtttttaag aattaattaa tat
//
LOCUS       DAOPYB010000347         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_75923_length_2140_cov_10.797122, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000347 DAOPYB010000000
VERSION     DAOPYB010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75923_length_2140_cov_10.797122"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKH58_13870"
     CDS             <1..795
                     /locus_tag="PKH58_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90159.1"
                     /translation="RLIALLYILLNQCSFSQSWVRIYGDTLNTYCYQVKEHYDKGYLF
                     VGYQTYDPGWASGWLLKTDINGYERWHKRYGSPTSNSPLFGMDRTSEGGMVMIGQTSL
                     QSNWWSAHVIKINACGETEWCRIYSTPGRQVYGLDIQSIPGGGSIAMVGNWGYDPTKI
                     LWLLRLDDNGDIVWQQTYATDTLFYDGEGYQLFRTADTNFLITGVVYSPDSGQFNPYK
                     QRPLIIKVRPDGTAVIEIAWGTALGFKGYGLVSVDDIKHNISLLSD"
     gene            complement(866..>2140)
                     /locus_tag="PKH58_13875"
     CDS             complement(866..>2140)
                     /locus_tag="PKH58_13875"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNX90160.1"
                     /translation="NNMGKNTEIKLVGQPIFKQVLDLIERVNIQSIVKKHDADRYYKA
                     FKAKTHLVTMLFGIFSRCDSMTEICEGLRAMGGKLNHLGLDKSPAKSTASDGLRNRPN
                     KFFEDVYFSLVDRYKSFLSDSRTYGLTFKEVLLIDSTTIRLFSDILKGVGRNPKDDGK
                     KKGGLKVHMLIDAVQSVGRFVKITAAKVHDKNFLKELELISHSMVVFDRAYNYYMQFA
                     LWTEQNIFFVTRMKKNAVYTVIETVQVHEKIKDKAMVIKEEIIELEYHPEDDRGKKVM
                     NQTNTIRLRRVCYQDEKNRYYEFLCNNFEISAEEIAFLYKKRWGIELLFKKMKQNFQL
                     HYFYGENVNAIYTQVWCTLIAQLLLTVIQKMAMTKKAFSVVASLVRIHLISMLDVFEL
                     LRSTKRYFSIKDHPLNQAVQLKFELKWGEVTI"
BASE COUNT          570 a          466 c          449 g          655 t
ORIGIN      
        1 agactgatag cccttttata cattctgctt aatcaatgtt ccttttccca atcgtgggtg
       61 agaatttatg gcgacacctt aaatacatac tgctaccagg ttaaagaaca ttacgataaa
      121 gggtatctat ttgtcgggta tcaaacatat gatcctggtt gggcatcggg gtggttattg
      181 aaaacggata taaacgggta cgaacgctgg cataaaaggt atggatcccc gacgtccaat
      241 tcaccgctat ttggtatgga cagaacttca gaagggggta tggttatgat tggccaaacc
      301 tcactgcaaa gcaactggtg gtcagcacat gtgattaaga ttaatgcctg cggcgaaaca
      361 gagtggtgcc gtatatacag cacacccgga agacaggtct acggactgga tatacaatca
      421 attcccggtg gcggctcgat cgcgatggtc gggaattggg gttatgatcc caccaagatc
      481 ctgtggctgc tccggctgga tgacaacgga gatattgttt ggcagcaaac ttatgcaacg
      541 gatactctgt tttatgacgg ggaagggtac cagttattcc ggacagccga tacaaatttc
      601 ctcattacgg gtgtggttta ttctcctgat tccggtcaat ttaatccata caaacaacgt
      661 ccactgatca tcaaagtacg accggacgga accgccgtaa ttgagattgc ctggggaacg
      721 gcattaggtt tcaaaggata tggcttagtt tcggtggatg atataaaaca taatatttca
      781 ctattatccg actaaagcct gatttctctt tttcagtttg ctaaagatca gataatcagg
      841 ccatattatt tgtaatttgg cattttcaaa ttgtgacctc cccccatttc agttcaaatt
      901 tcaactgtac cgcctgattt agcgggtggt cttttataga aaagtatcgt ttggtactgc
      961 gaagtagttc aaatacatca agcatactta tcaaatggat tctgaccagg gaagccacca
     1021 cggaaaaagc cttttttgtc atggccatct tttgaatgac ggtcagcagt agctgagcga
     1081 ttagtgtgca ccagacctgg gtgtaaatgg cattgacatt ttctccatag aaataatgaa
     1141 gttgaaagtt ctgcttcatt tttttgaata atagctctat tccccagcgt ttcttgtaga
     1201 gaaaagcaat ttcttctgct gaaatttcga aattgttgca aagaaattcg taataccggt
     1261 ttttctcatc ctgatagcag acccttctca gtcgaattgt gttggtttgg ttcatgactt
     1321 tcttgccacg atcatcttca ggatggtatt ccagttcaat aatctcttcc ttgatcacca
     1381 ttgctttgtc cttgatcttt tcgtgcacct gcaccgtttc aatgacagta tacacggcgt
     1441 ttttcttcat ccgggtgaca aaaaagatgt tttgctcagt ccacagtgca aattgcatgt
     1501 agtagttgta ggcacggtca aatacaacca tgctgtgaga gattaattcc agttctttca
     1561 gaaagttttt atcatgaacc ttggcagctg tgatctttac aaaccggcct accgattgaa
     1621 cggcatcaat gagcatgtga actttgaggc cgcctttctt tttcccgtca tccttcggat
     1681 tacgaccgac tcctttgaga atatcgctga acaaacgaat ggtagtagaa tcaatcagaa
     1741 gcacctcttt gaaggtaagt ccataagttc ggctgtccga caaaaaactt ttgtaccgat
     1801 ccaccaaact gaaatataca tcctcgaaga atttattcgg cctgtttctc aacccatcac
     1861 tggcggtgct ctttgccggt gatttatcca atccaagatg attcaatttt cctcccatcg
     1921 cccttagacc ttcgcagatt tcggtcatcg agtcacaccg gctgaagata ccaaacagca
     1981 tcgtcacaag atgtgtcttt gccttgaacg ccttgtaata gcggtctgca tcatgcttct
     2041 tgactatgct ttgtatgttc acccgttcaa ttaaatccag tacttgttta aaaatcggct
     2101 gtccgactaa ttttatttcc gtatttttac ccatgttgtt
//
LOCUS       DAOPYB010000348         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_77122_length_2119_cov_9.264535, whole genome shotgun sequence.
ACCESSION   DAOPYB010000348 DAOPYB010000000
VERSION     DAOPYB010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77122_length_2119_cov_9.264535"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..898)
                     /locus_tag="PKH58_13880"
     CDS             complement(149..898)
                     /locus_tag="PKH58_13880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX90161.1"
                     /translation="MHLPVLQLVNFVIILVVLVFLISYSWDIFFGETYEPPAWEKDRK
                     DKQLSPELLKFSKNYPDKVRLYNFWLQIQRLEKDMVTGYFAELGVYKGESARLLHLMA
                     PGRILHLFDTFEGFTEADLKPESGEAATYSTKNFADTSVEKVMKKIGGDSSKLKIHAG
                     YFPDSAKGLENETFALVNIDADLYNPVKAGLEFFYPRMSPGGIILIHDYNHHWEGLVK
                     AVDEFAAKTSCTIIPVPDLDSTVMIVCPKEE"
     gene            1138..1656
                     /locus_tag="PKH58_13885"
     CDS             1138..1656
                     /locus_tag="PKH58_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90162.1"
                     /translation="MKPKLIIAAVCLAVAGLTVPILISSCKNTGQKASDEAIEKTVEN
                     ATGKDVEVNSNNETMEISTNDGKVKYDGNIKSWPDDIPAEVPEFTYCKIDGATISDVD
                     NKIAYTFKIMDLNIDALKAYDKDLKAKGFESILMLIGEKGGTITAEKDGLSVALMGGD
                     GNGTLSVQIPKK"
     gene            complement(1696..>2119)
                     /locus_tag="PKH58_13890"
     CDS             complement(1696..>2119)
                     /locus_tag="PKH58_13890"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX90163.1"
                     /translation="GAVAKALKRIETKAVSPAPDIERLKEQYDASRGTIDRVVTRLGA
                     KVTVIAVDKIWYVESSDDYVMIYSEMGNHLKEKTMRYFEEHLPADTFIRIHRSYIINI
                     SQIAAIEPYTKDTHIVILKCGAKLRASSDGYKKLRAFL"
BASE COUNT          656 a          437 c          426 g          600 t
ORIGIN      
        1 aaacagccct cattttttga gggtgcaaaa atacaacatt attttaatta acaaatggtt
       61 gttagaaaaa tattgcaaaa atttgttcaa tcaatcgaaa atatagaatt gtaaagggtt
      121 tcaagcaaca aagggttaac aacacatttt attcctcctt agggcagaca atcatcactg
      181 tgctatccaa atctggcacc ggaatgatgg tacaggatgt tttcgcggca aattcatcca
      241 ccgcttttac cagtccttcc caatggtgat tgtaatcatg gatgagaatg ataccgcccg
      301 gcgacattcg cggataaaag aattcaagtc ctgccttcac agggttgtac agatcggcat
      361 cgatgttcac gagggcaaaa gtttcgtttt ccagcccttt ggctgaatca gggaaatatc
      421 cggcatggat tttcagtttt gatgaatcgc cgccaatttt tttcatcact ttttcaaccg
      481 aagtatcagc aaaatttttc gttgaataag tggccgcttc tcctgattca ggtttaaggt
      541 cggcttcggt aaaaccttca aatgtatcga acaggtgcaa aattctcccg ggagccatga
      601 ggtgcagtaa tctggcactc tcacctttgt aaacgcccaa ttcagcaaaa tagccggtca
      661 ccatgtcttt ttcaagccgt tgaatctgaa gccagaagtt ataaagccta accttgtcgg
      721 gataattctt tgagaatttc agcagttcgg gcgataattg tttatccttc ctgtcttttt
      781 cccatgccgg aggttcataa gtctcgccaa agaaaatgtc ccaggaataa cttattaaaa
      841 aaacaaggac caccaggatg atcacaaaat taacgagttg taatacagga agatgcataa
      901 gttagaattt ccagaacaaa attacagatt catgttactt taaaaagcaa atattttcaa
      961 aaattcaacc cgtttaaagc aaagccatct tttaacatct ttacacaata acttgccaga
     1021 gcaatctctc agtgaaatgc cggtggaatt acaattatta ggaataagta aattacttgt
     1081 tgattatcat tgatttacac attatatttg ttcattattc acaaatatca tgttcatatg
     1141 aaacctaaac taattattgc cgcggtatgc cttgctgttg caggattaac cgtcccgatt
     1201 ctgatcagct catgcaaaaa cacaggccag aaagctagtg atgaagcgat agaaaaaacc
     1261 gtggagaatg ctacgggtaa agatgtcgag gtaaactcga ataatgaaac catggaaata
     1321 tcgacgaatg acggaaaagt gaaatacgat ggtaacatca agagctggcc tgatgatatc
     1381 ccggctgaag ttccggagtt tacttattgc aagattgatg gagcaaccat cagcgatgtg
     1441 gataacaaaa ttgcttatac ctttaagatc atggatctaa acatagatgc attgaaagct
     1501 tatgataaag acctgaaagc caaaggtttt gaatccattc ttatgcttat tggagagaaa
     1561 ggcggaacaa ttacagcgga aaaggacgga ctgagtgtcg ctttaatggg tggagatggt
     1621 aatggcaccc tctcagttca aattcccaaa aagtaaattt ccagtctccg gttctcctgc
     1681 aataaacccc ggcacctaaa ggaatgccct gagtttttta tacccgtcgc tgctggcacg
     1741 cagcttggcg ccgcatttca ggataactat atgggtatcc ttggtgtaag gctcaatagc
     1801 tgcaatttgt gaaatattga taatataact cctgtgaatg cggataaaag tatcagcagg
     1861 cagatgctct tcaaagtatc tcatcgtctt ctctttcaga tgattaccca tctctgagta
     1921 aatcatcaca tagtcgtcgg acgattcaac ataccagatt ttatccactg ctatcactgt
     1981 aactttggct ccgagacgcg taaccaccct gtcgatcgta ccacgggatg catcatattg
     2041 ttctttcaga cgttcaatgt caggtgcagg agatactgcc ttagtctcaa tccgtttcag
     2101 ggctttggca acggctccg
//
LOCUS       DAOPYB010000349         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_78151_length_2101_cov_6.019550, whole genome shotgun sequence.
ACCESSION   DAOPYB010000349 DAOPYB010000000
VERSION     DAOPYB010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78151_length_2101_cov_6.019550"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKH58_13895"
     CDS             <1..185
                     /locus_tag="PKH58_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90164.1"
                     /translation="KYGKSVLLGACLKGSFVVPPQDDTIAEDDIARVLVILTIGRICL
                     FKGWCVFVTDPSLRSG"
     gene            complement(282..482)
                     /locus_tag="PKH58_13900"
     CDS             complement(282..482)
                     /locus_tag="PKH58_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90165.1"
                     /translation="MNNKGERDEFLQYTNDHNVMTRPIWNLMNRLLLYAHCQHDSLAN
                     SIWLADRVVNIPSSVIVEGYRK"
     gene            complement(520..1776)
                     /locus_tag="PKH58_13905"
     CDS             complement(520..1776)
                     /locus_tag="PKH58_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX90166.1"
                     /translation="MNKDTIKVLIAEYQQRVLDVSFVDRPYVMEENLNYVFVGLRRVG
                     KSYLMFQHIHHLINAGHVREEILYFNFEDDRIASLQTEDLDLIKVCYEEMFDHKPIFF
                     LDEIQIVPQWERFARRLADQGYRVYITGSNAKMLSSEIATTLGGRFVIRQVYPFSFGE
                     YLTLSHINISDPNAVYRYPTEISKSFESYFRYGGLPEVVQISDKRTWLSSLYQRIFFG
                     DLITHYQIRNDYALRVLIRKLSESVKQPTSFNRLANIVSASGKKISTDTVIDYLGYLK
                     ETWLVLQVENICAKLAEKESNKKYYFSDNGILNLFLIDPFTSLLENLVAIRLRQLYGE
                     NVYFYQKGVEVDFYVIDIQLAIQVSYSLLDPDTYKREINGLIKMAAHIEVNEMMIITK
                     DEEKIIAIDGYNIQVIPIWKWLLPGR"
BASE COUNT          561 a          441 c          448 g          651 t
ORIGIN      
        1 ataaatatgg caaatctgtt ttattgggtg cgtgcttgaa gggatccttc gtcgtgcctc
       61 ctcaggatga caccattgct gaagatgaca ttgctcgcgt gcttgtcatc ctgacgatag
      121 gaaggatctg tttgttcaaa gggtggtgcg tttttgttac agatccttcg ctacgctcag
      181 gatgacactt ttgctcaggg cgcgtgccaa ggggtccccg ctacccggga ctccgcttcg
      241 cttcgccttc actgcattgc attccgttca ggatgacaca gctacttcct atacccttct
      301 acaatcacgc tgctggggat attcaccacc cggtcggcaa gccagatgga gttcgcgagg
      361 ctgtcgtgct ggcagtgggc atacaggagc aggcggttca tcaaattcca gatggggcgg
      421 gtcattacgt tgtggtcgtt ggtgtattga agaaattcgt cgcgttctcc tttattgttc
      481 ataatgacgg cattcagcca atagttcgat ttgctgttgt taccttccgg gcagtaacca
      541 tttccaaatg ggtattacct gtatattgta tccatctata gcaataattt tttcttcgtc
      601 tttggtaata atcatcattt cattcacttc aatatgtgcg gccattttaa tcagtccatt
      661 tatttcgcgt ttataggtat cagggtccaa taaactgtac gacacttgta ttgctaattg
      721 aatatctatt acataaaaat cgacttcaac tcccttttga taaaaataga cattttcacc
      781 gtacaattgc cgcaaccgga tagctaccaa attttcgagc agcgaagtaa acggatctat
      841 cagaaaaagg tttaggatgc cattgtcact aaaatagtat tttttgttgg attctttttc
      901 cgccagctta gcacatatat tttccacctg caacaccagc caggtttctt tcagatatcc
      961 cagataatcg ataaccgtat cggtactgat cttttttccc gaagcagata caatattagc
     1021 caatcggttg aatgaagtgg gctgtttgac actttcggac aatttgcgga taagtacccg
     1081 caacgcataa tcgttacgga tttgataatg ggtgattaaa tcaccaaaaa atattcgctg
     1141 atacaaactg ctaagccaag tgcgcttatc ggaaatctga acgacctcgg gtaaaccgcc
     1201 gtacctgaaa tagctttcaa atgactttga aatttcggtt ggatagcggt aaacagcatt
     1261 cgggtcacta atattgatgt gtgatagcgt tagatattcg ccaaacgaaa aagggtaaac
     1321 ctgccggata acaaaccgtc cgccgagcgt agtggctatt tcgctgctta acatttttgc
     1381 attgcttccg gtaatgtaaa ccctgtagcc ctgatcggcc aatcgacggg caaatctttc
     1441 ccattgtggc acgatctgta tctcatcaag aaaaaagatc ggtttatggt cgaacatctc
     1501 ttcgtaacat accttaataa gatcaagatc ctcggtttgc agagacgcaa ttcgatcatc
     1561 ctcaaaatta aaatagagta tctcctccct gacatgaccg gcatttatca ggtggtgtat
     1621 atgctgaaac atcagatagg actttcctac gcgacgtaat cccacaaaca cataattgag
     1681 gttttcctcc attacgtatg gtcggtcaac gaaggataca tccagtactc tttgttgata
     1741 ttctgcaatt aatactttta tcgtgtcttt attcatttgt tttttatcga atatactcta
     1801 caaaaatagg atatatttat cgaatataaa agacagtttg ttgtttgttg tttgatgtgt
     1861 cgaggtgaga gggtgggcac tgagaaagtg ggcactgaga aagtgggcac tgagagggtg
     1921 agctctgaga gtgtgagcac tgagataatt agcgcgcgat caaaatgtcc atttttcggg
     1981 ataatgttgc atgtttacaa gcattcctat gatgttttca tattccaaat ctaattggtg
     2041 gtgtgtttct tctgaaatat attcacaatc caatgcaaaa tcaagccagt tttgtgtttc
     2101 t
//
LOCUS       DAOPYB010000350         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_78317_length_2098_cov_9.750367, whole genome shotgun sequence.
ACCESSION   DAOPYB010000350 DAOPYB010000000
VERSION     DAOPYB010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78317_length_2098_cov_9.750367"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..2008)
                     /locus_tag="PKH58_13910"
     CDS             complement(77..2008)
                     /locus_tag="PKH58_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX90167.1"
                     /translation="MKKILSILVVMLALVVFVQAQNKITRPNSHVNVPAKVAKVTGNE
                     AVPDNGIPQFIRTATTTAIGSTYYDAVTNGVARNTITNWTDGTVAAVWTMAQVSAQRG
                     TGYNYHNGTNWGPQPNPATDRIETVRTGWGVIAALNEGEIVAAHNGSTGLTINKRATK
                     GTGAWTESTLVGPGHDDEPENTGIYWPSIATQGDTVHLFACTDNVEGTLYQGIQTPLL
                     YYRSTDGGTTWSAPQLISAMANENKEMSDNYIAVAKNGKLVLLISEKFGETFYLESND
                     RGDTWTKHSVYPFAGGPNFDFTTGTFPMTALNDYAASVAIGDDGTVHVAFGTLMGART
                     EENAADEYSAWWSYDNIIYWNSTMPAFTVPFDTNTVEAYAGRIGRPNLDGDDTIWFMD
                     GYTQPEYRSNGPATFPQLVAEGGKVYMVYAALLEAPYLCGATTEYYHGVFATVSNDNG
                     TTWDDLNNVSWLSYHPEMYFVDWEQTELYGELMTISEGECFWPTMAHNSTNNKLNVMW
                     YFDYIPGQIGGFASSSSSVYAVNIDKNDVGDFKNTTEIYQNLWNAEGIKDNTLSELKL
                     FPNPTSNTVTVNLMSRETAQANLTVTNLMGQTVYSENISVETGNNQYQINVSDFGAGF
                     YMVNIQTKAGSTTQKLIVR"
BASE COUNT          688 a          388 c          360 g          662 t
ORIGIN      
        1 acaaataaat ctacaaatga aaaaaaaagg gtaaactttt gtttaccctt ttgaatatta
       61 taacaaatag ttgttgttaa cgaactatca atttttgagt tgttgatcct gcttttgttt
      121 ggatgttaac catatagaaa cctgcgccaa aatcagatac attgatttga tattgattat
      181 ttccggtttc aactgaaatg ttttctgaat aaacggtttg acccattaag ttagtaacag
      241 taagattagc ttgagctgtt tctctagaca ttaaatttac agtaacagta tttgaagtag
      301 ggttagggaa taattttaat tctgataaag tgttatcttt aattccttca gcattccaaa
      361 ggttttggta aatttcagtt gtgtttttga aatcaccaac atcatttttg tcaatattaa
      421 cagcataaac tgaagatgaa gaagaagcaa aaccgccaat ttgacctggt atatagtcaa
      481 aataccacat cacattaagt ttattgttag tagagttgtg agccattgtt ggccaaaaac
      541 attcaccttc actaatggtc attaattcac catataattc tgtttgttcc caatcaacga
      601 aatacatttc tggatgatag gataaccaac taacgttgtt caagtcatcc caagtggtac
      661 cattatcgtt agatacagta gcaaaaacac catgataata ttcagttgta gcaccacata
      721 aataaggagc ttctaaaaga gcagcataaa ccatataaac ttttccacct tcagcaacta
      781 attgagggaa agtggcaggt ccattacttc tgtattctgg ttgagtatag ccatccataa
      841 accagatagt atcatcacca tctaaatttg ggcgacctat tctacctgca taagcttcaa
      901 cagtattggt atcaaaagga accgtaaaag ctggcatagt tgagttccaa taaatgatgt
      961 tatcataaga ccaccatgca gaatactcgt cagcagcatt ttcttcagtt ctggcaccca
     1021 ttaaagtacc aaaagcaacg tgaacagtac catcgtcacc aatagcaaca gaagcagcat
     1081 aatcatttaa tgcagtcatt gggaaagttc cggtggtaaa atcgaaattt ggacctccag
     1141 cgaaaggata aacagaatgt tttgtccagg tgtctccacg gtcatttgat tccaaataga
     1201 aagtttctcc aaatttttca gaaatcaaaa gaactaattt accgttttta gcaacagcta
     1261 tatagttatc agacatctct ttattttcat ttgccatagc agaaattaat tgaggagcag
     1321 accaggtagt tccaccatct gttgatctgt aataaagaag tggggtttga attccttgat
     1381 aaagagtacc ttcaacattg tcagtacatg caaaaagatg aactgtatca ccttgtgttg
     1441 cgatgctagg ccaataaatt ccagtatttt ctggttcatc atcatggcca ggaccaacta
     1501 atgttgattc agtccaagca ccggtacctt ttgtagctct tttgttgata gttaaacctg
     1561 ttgacccatt atgagcagca acgatttcac cttcatttaa agcagcaata acaccccaac
     1621 cagtacgaac tgtttcaatt ctatcagtag caggatttgg ttgtggaccc caattggttc
     1681 cattgtggta attatagcca gttcctcttt gagcagaaac ttgtgccatt gtccatactg
     1741 cagcaacggt tccatcagtc caattggtga ttgtattacg tgctactcca ttggtaactg
     1801 catcataata agttgaacca atggcagttg tggttgcagt tctgataaat tgaggaattc
     1861 cattatctgg aactgcttca tttccggtta ctttcgcaac cttcgcagga acattaacat
     1921 gactattggg tcttgttatc ttgttttgtg cttgcacgaa aacaacaaga gccaacatga
     1981 ctactaaaat agataaaatt tttttcatag tataaaagtt tggttaataa tgtaaattca
     2041 cactacaaag atatacattt ttgtataaaa atgctacaaa ttgcaaaaaa aaatgtta
//
LOCUS       DAOPYB010000351         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_78563_length_2094_cov_10.547327, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000351 DAOPYB010000000
VERSION     DAOPYB010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78563_length_2094_cov_10.547327"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PKH58_13915"
     CDS             complement(<1..460)
                     /locus_tag="PKH58_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNX90168.1"
                     /translation="MRKVITYGTFDLFHQGHINLLRRAKELGDYLIVGVTTENYDKGR
                     GKLNVHRTLMERIEDVRNAGFADEIIIEEYEGQKIDDILRYEVDVFAIGSDWLGKFDY
                     LGEYCQVVYLERTKGVSSTQLRMEAQSVIELGVIGSGRIADRLIPESKYVS"
     gene            complement(797..1525)
                     /locus_tag="PKH58_13920"
     CDS             complement(797..1525)
                     /locus_tag="PKH58_13920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX90169.1"
                     /translation="MNNYILITGGTKGIGYAIAEILANSGYDLLLTYSSDQQQAITTQ
                     AELRDKYAVKVDVLQADISEKASIDKIASYLDTRDIHPFSVVFNAGLTCRTPFEEMTL
                     DEWERVFFANVHFPVFLLQRICSKLQSGGNVVFTGSLMGEYAHSVSLAYGVTKSAVHS
                     LVKNLVKFMQPYGIRVNAVAPGFVDTEWQKSKPAEIRKSIESKIALGRFCSPEELSEI
                     YKLIIENNYFNGEVITMSGGYSFR"
     gene            1869..>2094
                     /locus_tag="PKH58_13925"
     CDS             1869..>2094
                     /locus_tag="PKH58_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90170.1"
                     /translation="MIEKKKIFGFTPNVFYTGIVSFLTDTSVKMVYSVMPLFLMSIGA
                     SKTSLSLIEGIAESTASLIKAFSGYWSDRIG"
BASE COUNT          643 a          454 c          376 g          621 t
ORIGIN      
        1 cgctcacata tttcgattcg ggtataagcc tgtcggcaat tcttccactc ccgataactc
       61 caagttcgat aacagactgc gcttccatcc tgagctgagt actggataca cctttcgttc
      121 gctccagata caccacctga cagtattcac ccagataatc aaacttgcca agccagtcgg
      181 aaccaatggc aaaaacatca acctcatatc tcaaaatatc atcaattttt tgcccttcat
      241 attcctcaat aataatttca tcggcaaaac cagcattgcg cacatcctct atacgttcca
      301 ttaaagtacg atgcacattc agtttgccac gtcccttgtc gtagttttcg gtagtcacac
      361 caacgatgag atagtcacca agctcttttg cacgccgcaa cagattaata tgaccctggt
      421 gaaaaagatc gaaagtaccg tatgtaatga cttttctcat aattatcttt tataaataaa
      481 acctgtagcg cgtattacag tgtcagtccg gttgaattat gacatacaat taaaccgggg
      541 aaaatatgaa tattaaaaat ctgatatttc agcaggtaaa tatagtgaaa aaataaacta
      601 acgcgtattt ttatactgaa aaaacaactt ttcggctttt acaaactgca aagttagtat
      661 ttttttactt tattgaatga ttcatcaccc taaactgaca ccggtcagtt taatataatg
      721 atatttcggg ggggggacaa cacaatatga tttaattcat cttgcttttt acgttttgtt
      781 tgtggtgtcc ctaaaatcac ctgaatgaat aaccaccgct catggtaatc acctcaccat
      841 taaaatagtt gttttcgata attagtttgt atatctcaga caattcctcc ggactgcaaa
      901 accttccgag ggcaatctta ctttcaatgc tttttctgat ttctgcgggt ttgctttttt
      961 gccattctgt atccacaaaa ccaggtgcaa ccgcattgac acgaatacca taaggctgca
     1021 tgaactttac cagatttttt accagtgagt gtacagcaga tttggttact ccataagcca
     1081 gcgaaaccga gtgggcatat tcgcccatca gcgaaccggt aaacactaca ttccctccgg
     1141 attgcaactt gctgcaaata cgctgaagca gaaaaaccgg gaaatgaaca ttggcaaaaa
     1201 acacccgctc ccactcatcc agtgtcatct cttcgaaagg agtccggcag gtaaggccgg
     1261 cattgaacac taccgaaaac ggatgaatat cacgggtgtc gagatacgat gcaattttat
     1321 caatagaagc tttttcggaa atgtcagcct gtaacacatc cacctttaca gcgtatttat
     1381 cacgcaattc cgcctgagtt gttatcgcct gctgttggtc ggaagagtaa gtgagcagaa
     1441 gatcataacc ggaattggca agaatttcgg cgatggcata cccgatacct tttgtacctc
     1501 cggtaattaa tatataatta ttcattagtt tctgattgag aggtatatta ctgtaaattt
     1561 ccaaactgaa aacaagtaaa tcttttgacg gtgcgattgg ttgaactggc ttaaaaaacc
     1621 agttgcaaag ttaacgatta ttttacgatt gaaacagcat tgtaacataa aataaaaatt
     1681 tattctattg gtacttcact ccggatttgt gaaacacata ataatatccg gaaagaaaat
     1741 gtctgaaaaa agaattccca aatcatgttt tctgattaat tttgtattat cgttactgaa
     1801 atttttaatc cactgaatgg gttctgccga gttccaaaca gggactgcac acattcttct
     1861 aataaattat gattgaaaaa aagaaaattt tcggatttac gcccaacgta ttctataccg
     1921 gcattgtcag tttcctgacc gacacgtcag taaaaatggt ttattcagtg atgcccctgt
     1981 ttctgatgtc cataggggct tcaaagacca gtctttcgct catcgaaggg attgctgaga
     2041 gtaccgcttc actcatcaaa gcattttcgg gatactggag cgatcgcatc gggc
//
LOCUS       DAOPYB010000352         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_80240_length_2065_cov_7.090547, whole genome shotgun sequence.
ACCESSION   DAOPYB010000352 DAOPYB010000000
VERSION     DAOPYB010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80240_length_2065_cov_7.090547"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2065)
                     /locus_tag="PKH58_13930"
     CDS             complement(<1..>2065)
                     /locus_tag="PKH58_13930"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX90171.1"
                     /translation="LSIQNNGVQINLGLLPTTGFPYKILEGIKDFNVSTELFMNGFSL
                     GTSADGTLNGSFTNGDYKIGYSQGWEHFKGDIAEIILFKNALDSSQRASVENYLRFKY
                     AGQISLGPDISAYGFDSIILNAHSGFSNYLWNTGSADSLITVNQSGVYWVQANDLFDY
                     LTTDTVVVNYPVTRLSYSHDTTVCMGSPLVLTPLISPKNLYSFLWSNGATDTAISVNA
                     SGTYWVKITDSLGFYAYSDTIQVIIDSFAQGISLGNDTTFCSGNSIGLSPSLPGQASL
                     FFTWSDGSHDSTLTIINSGVYKLTVTNALGCSVEDSVYVAVSGTAPNTMFFADTVCLG
                     GYTTFTDISTFQPSDSIIKWHWQFNVSDTSNLQNPIYAFGTHGSHNVALTAYTPSGCS
                     QTNNLNIWVHPVPTPSFNAGVACINNPFQFTDLSTVVTGDSITFWQWDFGDSTPLSNQ
                     QNPVHFYNTSGNYTATLNVFSVNNCTQSVSHNIPVVNIYPDAATPQIFHPVDNAMLTG
                     TLVAFSWLPSANADRYKVIIASDSLFASILTQVLLDTTVFYYTFSSVNQTFFWKVLAY
                     NICSDSVASQTQRCEIFSPANIPGLNLWLKADTGVSLNGSQVMVWHDQSGQGRDAWQT
                     VPDQQPVFVTDELNGMPVLRFDGINDQINGLTIPEADSSSISIFVVAKGDDQSYVLAN
                     IFEINN"
BASE COUNT          636 a          430 c          440 g          559 t
ORIGIN      
        1 tattattaat ttcaaatatg tttgcaagca cataactttg gtcatcgcct ttagcgacaa
       61 caaaaatact tattgatgaa ctatctgctt caggtatggt aagaccgttg atttgatcgt
      121 tgattccatc aaacctcaaa acaggcatgc cattcaattc gtcagtaaca aaaaccggtt
      181 gttggtcggg aactgtttgc caggcgtccc ggccttgccc cgactgatcg tgccatacca
      241 ttacttgaga gccattgagg cttacccctg tatcggcttt cagccataaa ttcaagccgg
      301 gtatattggc cggagaaaat atttcacaac gttgcgtttg tgaggctact gaatcggaac
      361 aaatattata tgcaagtact ttccaaaaaa aggtctgatt aaccgaactg aaagtataat
      421 aaaaaactgt agtatccaaa agaacttgtg ttaatatgct ggcaaacagg ctgtctgatg
      481 caattataac cttataccgg tcagcattgg cagagggaag ccagctgaag gcaaccaaag
      541 tacccgtgag catggcatta tcaacaggat gaaatatttg aggagtggcc gcatccggat
      601 atatgttcac caccggaatg ttgtgagaca ctgattgtgt acaattgttt accgaaaaaa
      661 cattaagtgt cgccgtataa ttgccggaag tattataaaa atgaacgggg ttctgttgat
      721 tcgacaaggg tgtactatct ccaaagtccc attgccagaa ggtaatgcta tcgcccgtaa
      781 ccacagtgga caagtcggta aactggaacg gattattgat acatgctacc cctgcattaa
      841 aggatggtgt aggaacagga tgaacccata tattaagatt gtttgtctgc gaacaccccg
      901 atggcgtata agcagtcagc gctacgttat gagaaccatg cgtgccgaac gcatatatgg
      961 ggttttgtaa gtttgaagtg tcggaaacat taaattgcca atgccatttt ataatggagt
     1021 ctgaaggctg gaaagtagat atatctgtga aggtggtata acctcccaaa caaacagtat
     1081 cggcaaaaaa catagtattc ggtgctgtac cagatacagc aacatatact gagtcttcaa
     1141 cagaacagcc cagggcatta gtaacagtta atttgtaaac ccctgaattt ataatagtta
     1201 atgtcgaatc gtgggatccg tcagaccatg taaaaaacag gctggcctga cccggcaagg
     1261 aaggtgacaa accgatgctg tttcccgaac aaaatgttgt gtcattgccc aggctgattc
     1321 cctgtgcaaa agagtcaatg attacttgta tagtatccga ataagcataa aaaccaaggc
     1381 tgtcggttat ttttacccag taagtcccgc tggcatttac gctgatggcg gtatctgtag
     1441 cgccattcga ccacaaaaaa ctgtagaggt ttttcggact gataaggggg gttaatacca
     1501 gcggggagcc catacacacg gtggtgtcgt ggctatagct tagccgcgta acaggataat
     1561 ttacaacaac ggtatctgtg gtcaaataat caaacagatc attggcctgc acccaataaa
     1621 ctcccgactg attcactgtt atcaatgaat cggcactacc ggtattccaa agataattgc
     1681 taaagccgct gtgcgcattt agaataatac tatcaaaccc atacgcgctg atatcggggc
     1741 ccagagatat ttgtcccgca tatttaaatc gcagataatt ttcaacactt gcacgttgtg
     1801 atgagtctaa tgcattttta aacaaaatta tttctgcaat atccccctta aagtgctccc
     1861 acccctgaga ataaccaatt ttgtaatcgc catttgtaaa cgaaccgttc agggttccat
     1921 cagctgatgt acctaatgaa aagccattca taaacaattc ggtgctgaca ttaaaatctt
     1981 tgatgccttc cagaatttta taaggaaatc cggtggtggg taacaatccc agatttattt
     2041 gtactccgtt attttgaata ctcaa
//
LOCUS       DAOPYB010000353         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_81234_length_2048_cov_8.916708, whole genome shotgun sequence.
ACCESSION   DAOPYB010000353 DAOPYB010000000
VERSION     DAOPYB010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81234_length_2048_cov_8.916708"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..441
                     /locus_tag="PKH58_13935"
     CDS             118..441
                     /locus_tag="PKH58_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90172.1"
                     /translation="MNILPDFLKIQNEKRISLPRITINSHVHLRLPQCYATVTERKGW
                     WKLDNTNSLTSPVNGYDKASLTLVGSHVAVGGDEEEGDDFVLIGVETTMRLIIQLPRM
                     EWNSL"
     gene            583..2028
                     /locus_tag="PKH58_13940"
     CDS             583..2028
                     /locus_tag="PKH58_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase family protein"
                     /protein_id="HNX90173.1"
                     /translation="MNKRSLIKFLFCGYFIISGALVFAGSITVKGKVMSNGKAIPSVQ
                     ITDGKTIVLTDKNGKFNLETSGESEYVYYSLPSGYESTLVNGIPVFFQKINQNLKSQK
                     INFEIRKSEKSQSNHAFILWADPQVLDMEEFQQLEEVVADVKKTITAFPADMPVHAIS
                     AGDNVFDRLDFFDKYKQVISQTNVPFYQVIGNHDMDYNNRSNELSAKSFSAAFGPAHF
                     SFNLGKIHYVVLKDVFYYGFSYRYIGYVDENQLQWLEQDLKSVKPGSTVIVTLHIPTI
                     YGESEKAGSYTTEMSNSVLNRQALYRILSPYNSHILAGHSHTQWFTQATPNIAEHVHA
                     AASGAWWQGDVCTDGSPKGYTVYEINGDSLTWYFKGVNQNKTEQFKLYKAGTDAAFPD
                     CFIANVYGYDPQWKVIWYENGIKIGEMQRYWGEDPYAASLYQPGKNKKHSWLSVSQTH
                     HLFRAKLENPTAKILVEVIDRFGNKYKKTLE"
BASE COUNT          697 a          355 c          405 g          591 t
ORIGIN      
        1 aatcaaacaa gtgtacgtaa aatggtggtt tctaagtaaa agttaatttg tgttgaaaag
       61 cgataagagg gtgtttcttc aaaagagata ccctcttaaa gtaaaaaaaa ctgtttattg
      121 aatatcttgc ctgattttct gaaaatccaa aatgaaaaaa gaatatcttt accacgtatt
      181 actattaata gtcatgttca tttgcgatta cctcagtgct acgctacagt aacagaaaga
      241 aaaggctggt ggaaattaga caatacaaat tctttgacaa gtcccgtaaa tggatacgac
      301 aaagcatctc ttactttagt tgggtctcat gttgctgtag gaggagatga agaagaagga
      361 gatgattttg ttttgattgg agtggaaacc actatgcgct taatcatcca attgccccga
      421 atggagtgga atagtttata gcttaaataa atataatcaa tccagcggaa attacgtttt
      481 tgaatataaa ccagctcatt taaatccggg tgtttacgtt tatgaattac gaacgaaaag
      541 tcagttatta tccgggaaaa tgttaaaatc agaacgaaca ttatgaataa aagaagttta
      601 attaaattct tattctgcgg ttattttata atttccggag cattagtatt tgctggaagt
      661 attaccgtaa aaggaaaagt aatgtcaaac gggaaagcaa ttccgtcagt acaaattact
      721 gatggcaaaa ccatcgtatt gactgacaaa aacggaaaat tcaatctgga aacttccggt
      781 gaaagcgaat atgtttatta ttcccttcct tcgggttatg aatcgacttt ggtgaatgga
      841 attcctgtgt ttttccaaaa aattaatcaa aacctaaaaa gtcagaaaat caatttcgaa
      901 attcggaaat cagaaaaatc gcagtcaaac cacgctttta ttctttgggc tgaccctcag
      961 gtgcttgata tggaagaatt tcagcagttg gaagaagtgg ttgctgatgt aaaaaaaacc
     1021 ataacagcat ttccggctga tatgccagta catgctatca gtgccggtga taatgtgttc
     1081 gatcgattgg actttttcga caaatacaag caggttattt ctcaaaccaa tgtgcctttt
     1141 tatcaggtaa tagggaatca cgatatggat tacaacaacc gatcgaacga attatctgcc
     1201 aaatcttttt cagcagcatt cggacctgct cacttttcct tcaacttagg taaaatccat
     1261 tacgtagttc ttaaagatgt gttttattac ggcttctcgt accgatatat tggctatgtc
     1321 gatgagaatc aacttcagtg gttagagcag gatttgaagt cggtaaaacc cggaagcaca
     1381 gtgattgtta cacttcatat tcccacaatt tatggagaat ccgagaaggc cggaagttat
     1441 actaccgaaa tgtccaattc ggtgttgaac agacaggctt tatatagaat tctatcacca
     1501 tacaattcac acatccttgc cggacacagc catacccaat ggtttactca ggcaacgccg
     1561 aatatagcag aacacgttca cgctgctgcc agcggcgcct ggtggcaggg cgacgtctgt
     1621 acagatggat caccgaaagg ttataccgtg tatgaaataa atggcgatag cctgacatgg
     1681 tatttcaaag gagtaaatca gaataaaaca gaacaattta aactctataa agcaggtacc
     1741 gatgctgctt ttcccgattg ttttattgcc aatgtttacg gttacgaccc acagtggaaa
     1801 gtcatctggt atgaaaatgg aattaaaatt ggcgaaatgc agcgatattg gggtgaagat
     1861 ccttatgcag catcacttta tcagcccgga aaaaacaaaa aacattcctg gttaagtgta
     1921 agtcagactc atcatttgtt cagagctaaa cttgaaaatc caactgcaaa aatccttgtt
     1981 gaagtaattg atagattcgg aaataagtat aaaaaaacgt tagaataaat tttaattcaa
     2041 tgaagaaa
//
LOCUS       DAOPYB010000354         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_82211_length_2032_cov_10.841679, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000354 DAOPYB010000000
VERSION     DAOPYB010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82211_length_2032_cov_10.841679"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="PKH58_13945"
     CDS             complement(<1..1398)
                     /locus_tag="PKH58_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90174.1"
                     /translation="MNLMQYHYRHKAVLSFVALCFIACGSFVYGQVTPLQNVKQIREP
                     ETLPGLAVQQNEPEAGENPFVTYLSCDKSRDYYQVQMSELKTDFEKLYFLELAVSKKL
                     YIRSKSDWNLTSAVFYTKEYNREEAIKVMQELYRAAVIAEITMDNTSKAQYVASSEFI
                     PENSANQDARIEGPNANPICEDAEVACSQNTYTFPSGNQGTAPPPVNGYPYYGCLSSQ
                     PAPAWYFMQVQQNGDIIINIEQVDLTGSPIDVDFICWGPFTSLTAGCSNGLTAGNVVD
                     CSYSPDPFEVCDILNAQVGEIYILLMTNFAATSGHYGTITFSQTGGSGITNCDIVLNC
                     SILAITTDVSVCNPATNSFSVSGNIEFTNPPETGTLTITDSTALPVVSQTFTAPFNSP
                     LAYSLSNITCDGQQHALTAVFSADTSCKFTQTYNAPQASCPQATISGGGPVCDDGVST
                     VPVSINVTGSGPFTFI"
     gene            complement(1614..>2032)
                     /locus_tag="PKH58_13950"
     CDS             complement(1614..>2032)
                     /locus_tag="PKH58_13950"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX90175.1"
                     /translation="QLKKNIFEFTDFLVNILQVEDVGATFEHIVTFHDSCSALREYRL
                     TDQPRRLLANVRGLELREMKDSSVCCGFGGLFSVKHEAISAAMGGQKIENALNTGAEF
                     IVSTDSSCLMHQQGYIDKHKLPIKTMHIIDVLSSGW"
BASE COUNT          575 a          465 c          434 g          558 t
ORIGIN      
        1 aataaaagta aaaggccccg aaccggtaac attgatggat acaggaaccg tacttacccc
       61 gtcatcgcac accggacctc cgccgcttat tgttgcctga gggcatgatg cctgcggagc
      121 attatatgtc tgggtaaact tacaggacgt atcggccgaa aagacagccg ttaacgcatg
      181 ctgttgccca tcgcaggtta tattggacaa ggaataagcc aacgggctgt taaacggcgc
      241 cgtaaatgtc tgcgatacaa ccggtaaagc cgtactatcg gtaattgtaa gtgttcctgt
      301 ttccggcgga ttcgtaaatt cgatattccc ggaaactgaa aatgaatttg ttgccggatt
      361 gcaaactgaa acatcggtgg taatagcgag aattgaacaa tttagtacaa tatcacaatt
      421 agtgatgcca cttcctcccg tttgagaaaa agtgatagtg ccataatgtc cagatgttgc
      481 tgcaaaattt gtcatcagca aaatgtatat ctcgccaact tgggcattta atatgtcaca
      541 tacttcgaag gggtctggag aatagctgca atcaacaaca tttcctgccg tcaatccatt
      601 actacagcct gctgtaagac tagtgaacgg cccccaacaa ataaagtcaa catctatggg
      661 agatccggtc aaatcaactt gctcaatatt gattataata tcaccatttt gctgaacctg
      721 cataaaatac catgccgggg caggctgcga cgataagcat ccgtaataag gatatccatt
      781 gactggtggt ggggcagttc cttggtttcc cgaaggaaat gtgtatgtat tctgagaaca
      841 ggcaacttct gcatcttcac agatagggtt ggcattgggg ccctcaatgc gggcatcctg
      901 gtttgcagaa ttttcaggaa tgaattccga agatgccaca tattgcgcct tggatgtatt
      961 atccattgtg atctccgcaa ttaccgctgc acgatataat tcctgcatta ctttaattgc
     1021 ttcttcccgg ttatattctt tcgtatagaa aacagcagaa gttaagttcc aatcggactt
     1081 tgacctgata tatagttttt tcgatacagc aagctccaga aaatagagtt tttcaaagtc
     1141 cgtcttaagt tcagacatct gaacctgata gtaatccctt gacttatcac aagacaggta
     1201 tgtcacaaat ggattttcac ccgcttcagg ttcattttgc tgaacagcta gacctggtaa
     1261 cgtttcaggc tcccggattt gttttacatt ttgtaaagga gttacttgtc cgtaaacaaa
     1321 gctgccgcat gcgataaagc ataacgccac aaaactcaga acagccttgt gcctgtaatg
     1381 atattgcata agattcattt aatcaggaaa taacaaaagg agtatccaaa attactagtt
     1441 tttccgtttc gccggaaaaa aaccgaaaga tattcatcaa tcatgatttg aatgccctcc
     1501 ccatcccgga tgaacagccg gaacctttcg ggagattggc cctgatttcg aaaaacgaat
     1561 cagaacaacc tgttacagag cgttctgtat aaaacaataa gcccttttga tttttaccac
     1621 ccgctcgaca gcacatcgat aatatgcatg gttttaatgg gtagtttatg cttgtcgatg
     1681 tatccctgct ggtgcatgag gcatgacgag tcggtgctta cgatgaattc cgcaccggta
     1741 ttcaatgcat tttcaatttt ctgcccgccc atagcagcag atatcgcttc gtgctttacc
     1801 gaaaacagtc cgccgaatcc gcaacataca ctgctatcct tcatttcgcg cagttcgaga
     1861 cccctgacat ttgccagaag ccgcctcggc tggtcggtga gcctgtactc gcgcaatgcc
     1921 gaacatgaat cgtgaaaagt gacgatgtgt tcgaaggtcg ccccgacatc ttccacctgc
     1981 aggatgttca ccaggaagtc ggtaaattcg aaaatgttct ttttcaattg gt
//
LOCUS       DAOPYB010000355         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_85509_length_1980_cov_13.401558, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000355 DAOPYB010000000
VERSION     DAOPYB010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85509_length_1980_cov_13.401558"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(901..>1980)
                     /locus_tag="PKH58_13955"
     CDS             complement(901..>1980)
                     /locus_tag="PKH58_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90176.1"
                     /translation="VGMSHPAHFHLFHHTMEILKRDNHTVSVVITPKDVLEDLLKASD
                     INYIRLTERYDYSGFTGKLAKLVHSSVKLDRLVRDYQPDLMIGSFTQMAWVGRIRKIP
                     SFFFGEDDITYTFLQSLITYPFIKHIVAPLETKVGIFNYKTIAYNGTQKLACLHPNVF
                     VPDASRIPGVDVNHPFSIIRMVNLNAYHDVARKGLNSDILRRIIQKAETIGPVYISSE
                     KDLPEEFKNYRLPVAVSDIHHALAFATLFIGDSQSMAVEAAVLGTPALKCNDFAGKIS
                     ILNMLENNYGLTFGFKSSEAAALLKKMDELIATTDLKSVFANRRNKMLQDTIDVSAFF
                     AWLVGNYPQSISMIKENPDYQYKFR"
BASE COUNT          596 a          381 c          385 g          618 t
ORIGIN      
        1 tttttacagt ttcatgagta gcacaccact cctcaaaagt gataaaatga taaccggctt
       61 ctttcaaact aatgagcaga tgtcgatatg ttttaatggt aaaatccata tgtgttatag
      121 ggttatttaa aaatcattca attaatctga aagtcaggga tatactagat ttagtattcg
      181 taattatcaa agtggaataa ttgtgtaaat tttgcattta ttgctatatt atttatccag
      241 tgagaactct gtaagagttt tctgtgagta acccccggat ttatccgggg gaaagtgaac
      301 ctgtgaaaaa caatactctg taagagtttc ccaatgaagt catcctgact tttcattaaa
      361 ctaactaatt ttttaaaact acgaatgaca caaatttcac gaaaaaaaaa tcgtgttatt
      421 cgagaattga aaatcgacgt agtcaaatta gtagttagag tctgctaaaa atgcatatca
      481 agttataatt caaagaaaca tattggtata aattattctt aatgcaaaca ttttgaataa
      541 attacacaat attcttggca tcctgtttcg gtgcttccgt caagtcggaa tgtaccatct
      601 acaccttaat ttaagccaca gagtggcgta acacctgcct gcggcatgca ggtttgtaga
      661 aaataaatta tcctcgaatt ataaggtaca gagtaccgta acattttaca gttgagtttt
      721 tctgcagacc ctatgtattt gatataggac aacctcgatg aggttgtaac caagtttcat
      781 ttgcatcccc cggataaatc cgggggttac gcacagacta ctcctccgga gtgtaaatat
      841 cgtattattt gactaaatca tcttctatta tctattcaaa tagctatttt ttcatttaat
      901 ctatctgaat ttatactgat aatcgggatt ttcttttatc atcgaaatac tttgagggta
      961 attaccgaca agccaggcga aaaaagcgga gacatcgatg gtatcctgaa gcattttgtt
     1021 tcggcggttt gcgaatactg atttcaaatc cgttgttgcg ataagttcgt ccattttctt
     1081 caacaaggct gctgcttctg aacttttaaa gccaaaggtc agtccgtaat tgttttccag
     1141 catattcagg atgctgattt taccggcaaa gtcgttgcat ttcagtgcag gagtacccag
     1201 cactgcagct tcaaccgcca tactctgact gtcgccaata aataaggtgg caaatgccag
     1261 ggcatgatga atatcggaaa ccgcaaccgg taatctgtaa tttttaaatt cttcgggcaa
     1321 gtccttttct gaggaaatat aaaccggacc tatggtttct gctttttgga taatgcgacg
     1381 taaaatatcc gaatttaagc cctttctggc tacatcgtga tacgcattga gattaaccat
     1441 acggataatc gaaaaaggat ggttcacatc caccccggga atgcgcgaag catcgggtac
     1501 aaagacattg gggtgcagac aggctaattt ctgtgtgccg ttgtaagcta ttgttttata
     1561 gttgaatatc cccacttttg tttctaacgg cgcaactata tgttttataa acggataggt
     1621 gatcagactt tgcagaaagg tgtacgtgat gtcatcctct ccaaaaaaaa agcttggaat
     1681 tttacgtatc ctgcccaccc aggccatttg tgtaaatgag ccaatcatca gatccggttg
     1741 gtaatcccgc accagtctat caagtttcac cgaggagtga accagtttag ccagtttacc
     1801 cgtgaagccc gaataatcat atcgttccgt taatctgata taattgatat ctgaggcttt
     1861 gagcaggtct tcaagcacat ccttaggagt aatcaccacg ctgaccgtat gattatccct
     1921 tttcaggatt tccatggtat ggtggaacag gtggaaatgt gcaggatgac tcatgcctac
//
LOCUS       DAOPYB010000356         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_85642_length_1978_cov_22.962038, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000356 DAOPYB010000000
VERSION     DAOPYB010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85642_length_1978_cov_22.962038"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKH58_13960"
     CDS             complement(<1..403)
                     /locus_tag="PKH58_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90177.1"
                     /translation="MRRFIFFLLSIAVPLLVLADKYPEKTNDGNSPPPCHTVLLSKAN
                     VSCFGGSNGTVTATISKAGTYRVIWSNGVTINSTTSLTHQITSLSAGYYDVQVIDLVS
                     GCAAFDIIEVTQPALLTTSKTSVDVKCFGQST"
     gene            complement(412..>1978)
                     /locus_tag="PKH58_13965"
     CDS             complement(412..>1978)
                     /locus_tag="PKH58_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX90178.1"
                     /translation="YTSSWYNPSGQILNQNTLGLDSLVAGTYNTIITDAAGCTTDSSI
                     VISEPAPVTWHFTTTDNLCFGENNGSAIILTTGGIAPYSFNWSNGSTDTSATNLAAGT
                     YNVTITDNNGCIHTTSVIIQQPGSAVTATVEPTHILCFGYSGGTANLNPWGGTPPYSY
                     LWSNGSTTEDLSGVPAGTYTVTLTDNNGCIAYSGTVINEPSDSLSTQITIVNPSCHGF
                     ENGSISINPSGGTTPYYLRWDDTDFLISNTGHLLADLFDGSYQIIVTDANGCQNQQTV
                     TLIAPEALILDTTTTIVSCYGGSDGQIDMTVSGGTLPYSYLWSNGLSSEDLSGMTAGY
                     YSATVTDAQGCISKITCRINSRPEIEASSTTVPISCIDNADGSILVIPSGGTGDISYL
                     WSNGITENQIQNLAAGNYSVTLTDALGCMRNFDFTVPSSLFECIFIPSSFTPNDDGTN
                     DTWVIRNINLYPENIVKIFNRWGSLLYERSPYNETWNGTFKGKPLPSETYYYIVDLNN
                     GTKPFTGTVTIIR"
BASE COUNT          532 a          405 c          480 g          561 t
ORIGIN      
        1 ctgttgactg cccaaaacat tttacatcta ctgatgtttt gctggttgta agcaatgccg
       61 gttgtgtgac ctcgataata tcaaaagcag cacatccgct caccagatcg atgacctgca
      121 catcataata tcctgccgat agcgaggtga tctgatgcgt gagagatgtt gtcgaattaa
      181 ttgtgactcc attcgaccaa atcacccggt atgttcctgc cttgctgatg gtggccgtta
      241 ccgtgccatt ggaccctcca aaacagctta cgttagcctt ggaaagtaaa acggtatggc
      301 acggaggtgg actattgccg tcatttgttt tttcagggta cttatcggct aagacaagca
      361 aaggtacagc aattgacaaa aggaaaaaaa taaatcgtct catatatata attatctaat
      421 tattgtaact gttccggtaa aaggcttcgt tccgttgttc aaatcaacaa tataataata
      481 tgtttcagaa ggcaggggct tgcccttaaa agttccattc catgtttcat tatagggcga
      541 tcgttcatag agaaggctgc cccagcggtt aaatattttt actatgtttt cggggtagag
      601 gtttatgttc ctgatcaccc aggtgtcgtt tgtgccatcg tcattgggtg tgaaagatga
      661 tgggataaaa atacattcaa aaagactgga aggtacggtg aaatcaaaat tccgcataca
      721 tccaagtgcg tcggttaaag taacagagta atttcccgca gcaagatttt gaatttgatt
      781 ttcagtaatt ccatttgacc ataggtaaga gatatctcct gtgccacccg atgggattac
      841 cagtatgctt ccgtcggcat tatcaataca actgatgggg acagtagttg aagacgcctc
      901 aatctcaggc ctgctattta tccggcaggt aatttttgag atgcaaccct gggcatcagt
      961 aactgttgcg gaatagtatc cggcagtcat acccgagagg tcttcactgc ttaaaccatt
     1021 agaccataaa tagctgtacg gtaatgttcc tccgctgaca gtcatatcaa tttggccatc
     1081 cgatccgcca tagcaactta caattgttgt ggttgtatca aggataagcg cttcgggggc
     1141 aataagtgtt actgtttgct ggttctgaca accattggcg tcagtaacga taatctgata
     1201 agacccgtca aaaaggtcag cgagcagatg tcctgtattg cttatcagaa aatcagtatc
     1261 gtcccatcgc agataatagg gagttgttcc gcccgatgga ttgatgctga tgcttccgtt
     1321 ttcaaaccca tgacaactgg ggttaacgat ggttatctgt gtggaaagtg aatctgacgg
     1381 ttcgttaatt acggtaccgc tgtatgcaat acacccatta ttgtcggtaa gcgtaacggt
     1441 atatgtcccg gcaggaacac ccgacaggtc ttctgtggtt gatccgttag accacaaata
     1501 cgaataaggt ggcgttcctc cccatggatt aaggttggct gtgccgccgg agtaaccgaa
     1561 acataatata tgtgtaggct ctacagtggc tgtgacggca gaaccgggct gctggattat
     1621 tacggatgta gtatgaatac aaccgttatt gtcggtgatg gttacattat aagtccctgc
     1681 tgccaggttg gttgcagagg tatctgtaga gccgttcgac cagttgaaag aatatggggc
     1741 tatcccgcca gtggttaaaa tgattgccga tccattattt tctccgaaac aaaggttgtc
     1801 ggtagtggta aagtgccatg ttacaggagc cggctcagaa ataacaatgc ttgaatctgt
     1861 ggtgcatcct gccgcgtcgg taataatggt gttgtaagtt cctgcaacaa gggagtcaag
     1921 gccgagggta ttctggttaa gaatctgtcc cgagggattg taccatgagg atgtgtat
//
LOCUS       DAOPYB010000357         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_88553_length_1936_cov_7.311005, whole genome shotgun sequence.
ACCESSION   DAOPYB010000357 DAOPYB010000000
VERSION     DAOPYB010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88553_length_1936_cov_7.311005"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1922)
                     /locus_tag="PKH58_13970"
     CDS             complement(<1..1922)
                     /locus_tag="PKH58_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX90179.1"
                     /translation="MGYVSQTIPVQGKSLINVTMSEDTKTLDELVVIGYGTVKKSDLT
                     GAVGSIKSSDITKVAASNALQAIQGKVSGVDIQQSSGQAGAGVSINLRGNRSITASNG
                     PLILVDGVEYGSTIDINPSDIESMEILKDASSTAIYGTRGANGVVIITTKRGKAGATK
                     VTVNSYLASNSPVNIPKVMYGDKEVQRLLDKANYSADFAAYKLDPNYVWGTSNRTPEQ
                     ELTLSLEDFTEIGIYNDKSYTNWLDIILQNGLTKNIETSVSGGNEKTNFNISLGGMFE
                     EGMMKNDKQDRYNVKTTIDHRINKLFKTGSSLLFTYKDRDARNASVFTQAMKMTTITH
                     PYRADGSIIETPNPRYAAHCNPLLDEIPGNYVNNIESTRLFGNGYLEIDPIKNAIFKS
                     SLTVDRSNARTGLYQDYQSVSRYQAPASGYISSEYNNNTKYTWENTLTYNTNFGNSKH
                     DLTGLLGHSMSQSVYESTKTYGDAGKEHYYTSLFYDLKKIGSPTTETGYIKQAMLSYF
                     GRINYKFNEKYLLTASIRADGSSTLAPGHKWGYFPSAAVAWRVNQEDFLKDVSWLNNL
                     KLRASWGVSGNSAVDPYATMSTLSALPVYYYLGGKSIAGYYPSVLGNSTLKWETTDAR
                     NFAVDFGVLNNRISGSV"
BASE COUNT          528 a          414 c          379 g          615 t
ORIGIN      
        1 acgctacccg aaatgcggtt gttcaataca ccgaagtcaa ctgcaaagtt cctggcatcg
       61 gtagtttccc atttcaaggt gctatttccc aacacacttg gataataacc tgctatactt
      121 tttccgccaa gataataata tacgggtaat gcactcagag ttgacattgt agcataagga
      181 tcgactgctg aattaccgga aacaccccat gatgcacgta atttcagatt attgagccag
      241 ctaacatctt tcaggaaatc ttcctgatta acacgccatg ctacagctgc tgatggaaaa
      301 tatccccatt tatgaccagg agccagggtt gatgaaccgt cggcacggat agatgcagtt
      361 aagaggtatt tttcgttaaa tttatagttg atacgaccaa aataagacaa catcgcttgt
      421 ttaatataac ctgtttctgt ggttggtgaa ccgattttct tcaagtcgta aaacaaactt
      481 gtataataat gttcttttcc ggcatcacca taggttttgg ttgactcgta aacactctgg
      541 ctcatactgt gtccgagtaa tccggtcaga tcgtgttttg aatttccaaa gtttgtatta
      601 taggtcaaag tattctccca ggtgtatttg gtgttattgt tgtactccga agaaatataa
      661 cccgatgccg gagcctgata acgtgataca ctctgataat cctgataaag tcctgtacgg
      721 gcattcgacc ggtcaacagt cagactggat ttgaatatcg cgtttttaat gggatcaatt
      781 tccagatatc cgttgccaaa caaacgggta ctttcaatat tattcacata atttccgggt
      841 atttcatcca gtaaggggtt acagtgtgct gcataccgtg ggttgggagt ttcgataatg
      901 ctgccatctg cacgatacgg atgtgtaatg gtagtcatct tcatcgcttg tgtgaataca
      961 cttgcatttc gtgcatcacg atctttatag gtaaataaca aactgctacc ggttttgaat
     1021 aatttattga tccggtggtc tatggtcgtt tttacattgt aacgatcctg tttatcattt
     1081 ttcatcattc cttcttcaaa catacctcca agcgaaatat tgaaattggt tttttcgtta
     1141 ccacccgaaa ccgatgtttc gatatttttg gtcaggccat tctgtagtat aatgtccagc
     1201 cagttggtat atgatttatc gttataaatt cctatttctg taaaatcttc cagcgataaa
     1261 gtcagttcct gttccggagt tctgttgctg gttccccaaa cataattcgg atccagttta
     1321 taagcggcaa agtctgctga ataatttgcc ttatcgagca aacgctgaac ttctttatct
     1381 ccgtacatga ctttcgggat attgacagga gaatttgatg ccagatatga atttacggtt
     1441 actttggtgg cacctgcttt acctcgtttg gttgtaataa tgacaactcc gttggcacca
     1501 cgtgtaccat aaatagctgt tgaagaagca tccttcaata tttccataga ctcaatatcc
     1561 gatggattaa tatcaatggt ggagccgtac tctactccgt caactaaaat aagcggaccg
     1621 tttgaagcgg taatggaacg attgccacgt aaatttatgc ttacaccagc accggcctga
     1681 ccgctcgatt gctgaatatc cacaccggaa accttaccct gtattgcctg caaggcattg
     1741 ctggcagcta ccttcgtaat gtcactgctt ttaattgagc ctacagctcc ggttaaatca
     1801 ctttttttaa cagtaccata accaatcacc accagttcgt ccagcgtttt tgtgtcttca
     1861 gacatcgtta cattgattaa ggatttaccc tgtacaggta ttgtttggga tacataaccc
     1921 acataggaaa aataca
//
LOCUS       DAOPYB010000358         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_88910_length_1931_cov_8.535714, whole genome shotgun sequence.
ACCESSION   DAOPYB010000358 DAOPYB010000000
VERSION     DAOPYB010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88910_length_1931_cov_8.535714"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..898)
                     /locus_tag="PKH58_13975"
     CDS             complement(149..898)
                     /locus_tag="PKH58_13975"
                     /inference="COORDINATES: protein motif:HMM:NF040365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6261 family protein"
                     /protein_id="HNX90180.1"
                     /translation="MNILKTKINRMRSEDVATVGIRVIETIEKSVAEEVKNSLLFTQL
                     KEVTNRYRKAIEPDDKEEKAVVTEKFNFRKQLFNNFYNFVAGLKLSKDPTVQAAATVV
                     FSVLNMYGGLGFRDLSRTAHTQRYTTIVETLSKAEYTEAIAKLNVGDYLSELADANAA
                     YETVYQAKGNKRSMRIPSNDMRTEMNNALKDITEELRLFVRKYPTELNKELQNNVMQR
                     IAEVYVPAPGSVKKVKTSTESTNQVSTTEVI"
     gene            complement(1186..1581)
                     /locus_tag="PKH58_13980"
     CDS             complement(1186..1581)
                     /locus_tag="PKH58_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90181.1"
                     /translation="MMSNKDNSDLAFNENYDLLYPTLLKMEVSDYPKHGKSLPKATNF
                     RIPDYYADPLCVTQSATHFIYFVNLYARSYNNKRRLMHQSLSRIIRSFFKRWNDNMHY
                     HLTLLFGLSNIRPANLYNAIFGSSSYRIH"
BASE COUNT          563 a          385 c          378 g          605 t
ORIGIN      
        1 ttaatcaaag aacaatgtca gtccccggga tgaatatatt acatacaaaa aaaaagaacc
       61 ctgcagattt ctctacagag ttcttcgtca atgattattt acaatcagaa gttgtcagct
      121 tttcgtccga caatcttcca tagcgctctc aaatcacttc cgtagtgcta acctgatttg
      181 tactttcggt ggatgttttc acctttttga cagatccggg agcaggaaca tacacttcag
      241 caatacgttg cataacgtta ttctgcagtt ctttgttaag ctctgtggga tatttgcgta
      301 caaataatct cagttcttct gttatgtcct tcagtgcatt attcatctcc gtacgcatat
      361 cgtttgacgg aattcgcatg gaacgcttgt tacccttagc ctgatacacc gtttcataag
      421 ccgcattagc atccgccagt tcactgaggt agtcacccac attcagtttg gcaattgctt
      481 cggtatattc agctttgctc aaggtctcaa cgatggttgt gtagcgttgg gtatgagctg
      541 tccggctcaa atctctgaac cctaagccac catacatgtt aagtactgaa aacaccacag
      601 tggctgctgc ttgtaccgtc gggtctttcg acaatttcag cccggcaacg aagttgtaaa
      661 agttgttgaa cagctgtttc cgaaagttga acttttcggt aacaacagct ttttcttcct
      721 tatcgtccgg ttcgatagct ttgcggtatc ggttagtcac ttcttttaat tgtgtgaata
      781 acaaactgtt tttcacttct tccgccaccg atttctcaat ggtttctatt acgcgaattc
      841 caaccgttgc tacgtcttcg cttcgcatac ggttaatttt agtttttaat atgttcataa
      901 tgaaattttt atatttattt tttggcctcc aacactctcc gttcgttcca ctgtgcccgg
      961 cagcttgctc accgtatatc actgtaataa aatgcaatca ctataaaaac aaattgaatt
     1021 atcagtgtat gttttcggaa aaagcaggac tactattgag ccttgatcct ataactgaac
     1081 ttagcggttg tcagccggta aaaaaaaagt attgtttgat taaacttaca gttgaggatg
     1141 tcaactgcgg tgtcccgata accgggacat actgcgggta gaaagttagt gaatcctgta
     1201 ggaggaagac ccgaatatgg cgttgtacaa attagccgga cggatgttgc tcagtccgaa
     1261 aagtaaggta agatgataat gcatgttatc attccatcgt ttaaagaatg atcttattat
     1321 gcggcttaac gattgatgca tcagccggcg tttgttgttg taactgcggg catagaggtt
     1381 tacaaaataa ataaaatggg tagcactctg ggtgacgcaa agtggatcag cgtaataatc
     1441 gggtatgcga aagtttgttg ctttgggtag tgactttcca tgtttcgggt agtcgctcac
     1501 ttccatcttt agcaaagtgg gatatagtaa atcgtagttt tcattaaatg ccaaatcgga
     1561 attatcctta ttgctcatca taatcttcca aaaaggagac atcaaaacct tccttatcag
     1621 atgcctgaca agatggcagt aaatgcggaa aatatggagc ttttggcaaa acatttttat
     1681 gatttaagaa atttaataac tgttatcata ctacaataat catgccaaaa gccaataatt
     1741 cataaatatc attaaaacga taattaaatg gcagaaaacc ggacagaatg gcaaagaatc
     1801 ggacaactct gacactgagt tcatcttgat tttttcaata aagctatgat gtcgcagcta
     1861 cagagctcaa tattaggttt aatcgttaat cgtgtgaaat aaaaaaataa tctaacacca
     1921 gagatagcaa a
//
LOCUS       DAOPYB010000359         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_90788_length_1906_cov_5.419773, whole genome shotgun sequence.
ACCESSION   DAOPYB010000359 DAOPYB010000000
VERSION     DAOPYB010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90788_length_1906_cov_5.419773"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..767
                     /locus_tag="PKH58_13985"
     CDS             378..767
                     /locus_tag="PKH58_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90182.1"
                     /translation="MSEQESHYRELIRLLLPEELFDYFTVVSIKVEDKSVHVFLDEID
                     DKPEAYLNEKLTSKGFHAEVVVQDFPIRKKAVFLHVRRRKWLVEATGKLVSKSWNLTA
                     EGTRYTKSFADFLKGIFGQLSDKQQQP"
     gene            733..1716
                     /locus_tag="PKH58_13990"
     CDS             733..1716
                     /locus_tag="PKH58_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX90183.1"
                     /translation="MDNYPISSNSLEKHYYIDGDRFGQQYKEHLSSYKDWDQKDHAQE
                     WILYSENLGPRLSIDETALSNGDLYTVVTNKDGKGGEGTIVAMIEGTTSEKVIKVLNS
                     IPEKEREVVKEVTLDMAGSMDKIVRGCFPCATRVIDRFHVQKLTNDAIQEIRIGHRWD
                     AINQETNAKQNAKLDGKKYKPEELSNGDTTKQLLARSRYLLFKSAEKWTPTQKERAKL
                     LFELYPDIKQAYWLAHGLRMIFTNTKNKGVAYTKMAKWFNDVTDSDIKSFNTITATFY
                     SHYPEILNYFDNRSTNASAESFNAKIKAFRATQRGVRDIPFFLFRLAKIYA"
BASE COUNT          638 a          355 c          407 g          506 t
ORIGIN      
        1 aaaacgtaac attcattgtt aagaagcatg tgagaagcaa attttagctc tatcccaata
       61 atccgaaaca atgttaattt gagaaattga tatgcaggta ttttatcggc tcaataaagg
      121 gtaaaaaatg aaccaattag caaatacaca tgcatcatat tggacgaact atgattttaa
      181 agtccatagt tggaaatgtg ctgttttggg cacaaactaa ggaggtattt tacctccttt
      241 tttcttttat aagcagatcg ttaagtataa aacggatcaa gcggaaaagt ggatcaagcg
      301 gtatttctgg gatcaaatga attttctggt ggagaaggaa taaattttga tctttgtttt
      361 gaacaaagct ttaaccaatg tcagaacaag aatcacacta tcgggagtta atccgattat
      421 tattaccaga agagttattc gattacttta ctgtggtcag tataaaagtt gaagacaaat
      481 cagtgcatgt attcttggat gagattgatg ataaacctga ggcttacctc aatgagaagc
      541 tcacatcaaa aggatttcat gccgaggttg ttgtccaaga ctttccgatc cgaaagaagg
      601 cagtattcct tcatgtgcgc aggcgaaagt ggttggttga ggccacaggg aaattggtca
      661 gtaagtcatg gaacctgaca gcagagggga cgcgttacac caaaagtttc gctgattttt
      721 taaaaggaat atttggacaa ttatccgata agcagcaaca gccttgagaa acattattat
      781 atcgatggag accggtttgg gcagcagtac aaggaacatt tgagcagcta caaggactgg
      841 gatcaaaaag atcacgccca agaatggata ctttactccg agaacctggg cccccgcctg
      901 agcattgacg agactgccct ttcaaacggg gacctttaca ctgtggtgac caataaagac
      961 gggaaagggg gcgaaggcac aattgtggca atgatagaag gcacaacatc cgagaaggtt
     1021 attaaggtgc tcaatagtat ccctgaaaaa gaacgggaag tggtcaagga agttacactg
     1081 gatatggctg gctcgatgga caagattgtg agagggtgtt tcccatgcgc cacaagggta
     1141 atcgaccgtt tccatgtaca aaaactgacc aatgatgcca ttcaggagat acgcataggc
     1201 catcgttggg atgcaataaa ccaagagacc aatgccaaac aaaacgctaa acttgatggt
     1261 aaaaaatata aaccggaaga attgtccaat ggggacacca caaaacagct actggcacgc
     1321 agccgctacc tgttgttcaa atcggctgaa aaatggacac caacgcagaa agaaagggct
     1381 aaattgttgt ttgaactcta ccccgatata aaacaggcat actggctggc acatggacta
     1441 aggatgatct ttacaaacac caaaaacaaa ggagtggcat atactaaaat ggcaaagtgg
     1501 ttcaacgatg taacagactc tgatatcaaa tctttcaata caataacggc aaccttctac
     1561 tcacattatc ctgaaatatt gaactatttt gataatcgta gcactaacgc ttcggctgaa
     1621 tcattcaatg caaaaatcaa agctttcaga gcaacccaaa gaggtgttag ggatatccca
     1681 tttttcctgt tccgattagc taaaatatat gcttaaaaag tatagctcca ccagattgtt
     1741 gaattgatcc ctctttaacc tatcaaaccg atttgtttat attactcatc cactttcttt
     1801 ccattaggtt tcatgttttt agtacctctt ttgcatgatg acaaaatggc tcaattatga
     1861 catgagtatg acatttaagc gaatttggta tgtctttgat atgact
//
LOCUS       DAOPYB010000360         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_90857_length_1905_cov_10.950811, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000360 DAOPYB010000000
VERSION     DAOPYB010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90857_length_1905_cov_10.950811"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1229)
                     /gene="uxuA"
                     /locus_tag="PKH58_13995"
     CDS             complement(60..1229)
                     /gene="uxuA"
                     /locus_tag="PKH58_13995"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HNX90184.1"
                     /translation="MKKTWRWFGRKDRITPDMLRQIGVEGIVTSLHDVPNGEIWTSEA
                     IEEVKSYIESFGLRWSVVESLPVSESIKYAGPDRDRLIENYKVSLANLGKAGIKTVVY
                     NFMPVIDWIRTDLNYKLPNGTYTLFFDKIRFAYFDCYILNRPQAEKDYSAEEMEQVNE
                     LKKTITEAEKEELVNTIIVKTQGFVNENFKGNVSKPVELFNDLLALYQGVDKQQLREN
                     LKYFLNEVIPIAQKYDVNLCIHPDDPPFPVLGLPRIVGNTEDIEWILNAVDIPNNGFT
                     FCAGSLSSGLHNDVPLMAQKFVKRTHFVHLRSTDVAENGNFIEASHLEGRGHLVELVR
                     IFEREHPGLPMRVDHGKLMLDDVEKSDNPGYSFYGRMYALAEVSGIMAAVKDSLK"
     gene            complement(1284..1478)
                     /locus_tag="PKH58_14000"
                     /pseudo
     CDS             complement(1284..1478)
                     /locus_tag="PKH58_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1638..>1905)
                     /locus_tag="PKH58_14005"
     CDS             complement(1638..>1905)
                     /locus_tag="PKH58_14005"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HNX90185.1"
                     /translation="PDSKLKKPIRSLQGFKRIHLKKGETQTVEFSLKPAQFAARDDAN
                     LPVVQAGDVLLSIGGKQPDEKSIANRKVIQKIVKLRGDNLFIND"
BASE COUNT          520 a          452 c          342 g          591 t
ORIGIN      
        1 gccacttttc cttcaatact aaattgttcg ttcatcattt ttatttacta ttttaatatt
       61 tactttaaac tatcttttac agctgccata atgcctgaaa cttcggccag cgcatacatc
      121 cgtccgtaga atgaataacc cggattatcc gatttctcca catcgtcgag catcagttta
      181 ccatgatcca cccgcatggg caggccagga tgttcgcgtt cgaaaatacg caccaattcc
      241 accagatgac cgcggccttc gaggtgtgat gcttcaatga aattgccatt ctctgcaaca
      301 tcggtactcc gaaggtgcac aaaatgggtt cgcttgacaa atttctgcgc cataagagga
      361 acatcgttgt gcaaaccgga gctaagcgag ccggcacaaa acgtaaatcc gttgttggga
      421 atatctacag cattcaatat ccattcaata tcttcggtat tgccgacaat gcgtggtaat
      481 cctagtaccg gaaacggcgg atcatcagga tgaatgcaga ggttgacatc atacttttgg
      541 gcaatcggaa tcacttcgtt gagaaaatat ttcagatttt cacgaagctg ttgtttatcc
      601 actccctgat ataatgccag taaatcgtta aacaattcga ccggtttact cacatttccc
      661 ttaaaattct cgttgacaaa tccttgtgtt ttcacaatga tcgtattcac caactcttct
      721 ttttcggctt cggtgattgt ttttttcagt tcattgacct gctccatttc ttctgctgaa
      781 taatcctttt cagcctgagg gcggttgaga atgtaacaat caaaatatgc aaaacgaatt
      841 ttatcaaaga acagagtgta agtaccgtta ggaagtttgt aattcaggtc tgtgcgtatc
      901 cagtcaatga cgggcataaa attgtaaacc acggttttaa tacccgcttt ccccaggttt
      961 gccagactta ctttgtaatt ttcgattaat ctgtcccggt cgggaccggc atatttaatg
     1021 ctttcgctca ccggcaaact ttcgaccacc gaccagcgta gtccaaaact ttcgatatag
     1081 ctttttactt cctcaattgc ctcagatgtc catatttcac cattcggcac atcatgcagg
     1141 gaggtcacaa tgccttccac cccgatttgc cggagcatat cgggtgtaat ccggtctttt
     1201 ctcccaaacc atctccacgt ttttttcatg ttattctttt tgtaattttc ccaaagttag
     1261 caattcaaga aaaattttgt tcattaattg tgatgcgtta aattcagttt aatttttttt
     1321 aaactatcca gatcatgtac cggacgctcg atatcatacg gaattggtag ttttgagaaa
     1381 gtctgaaaat aaagcagaca agcatctttc caccaaaccg catcgcgtgc ctgtacgcga
     1441 agtttcgact gtacttcgcc gaaacgctga ctgtcaatac attcttatag gcacaaattc
     1501 ctgaaaaaag gtcttcctta catttgtctc cgcaaagcga agccataaac gactgccgtt
     1561 ttcagcacac acactaaaag cgacagccgc catgagaaaa cacaaaaaca atcttttttt
     1621 acacttaaaa ctcataccta atcattaata aacaaattat cacctcttaa ttttactatt
     1681 ttctgaatta cttttctatt tgcaatcgat ttttcgtcgg gctgtttacc cccaatgctc
     1741 agcagcacat cgcccgcttg aactacgggc agatttgcat catcgcgggc agcaaactgc
     1801 gccggtttta aagagaattc cacagtttga gtttcccctt ttttcaggtg aattcttttg
     1861 aaaccctgca acgaacgaat gggctttttg agtttgctgt ccgga
//
LOCUS       DAOPYB010000361         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_90938_length_1904_cov_6.977285, whole genome shotgun sequence.
ACCESSION   DAOPYB010000361 DAOPYB010000000
VERSION     DAOPYB010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90938_length_1904_cov_6.977285"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..>1904
                     /locus_tag="PKH58_14010"
     CDS             325..>1904
                     /locus_tag="PKH58_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNX90186.1"
                     /translation="MELKILKPRKALNKAFLKVKPSRTEIECFKTNLSHLLDSINDTG
                     SEEFHKNLVSDFLKDTYYKQSHFVNTKGNCDLVIHNADKATSTVGVIIEAKKPTNKAE
                     MLTLDKINVKAFQELVLYYLRERITQKNIEIKYLIATNIYEWFIFDVTQFDRLFAQNK
                     NLVKQFIDFESGRLADTKTDFFYKQIAENFIAEIKHEVEFTYFDIRDYEKALRNADKK
                     DDNQFIPLYKLLSPQHLLKLPFTNDSNSLDKRFYSELLHIIGLTETKEGSKKLIERNK
                     EGQRNSGSLLENAIIQLDSLDKISRIENPGHFGSNPQERLFNVGLELSITWINRILFL
                     KLLEAQLKTYHKGDKSYEFLSFSKIKDYDDLNSLFFQVLARRQNERSDDVKTLFAKVP
                     YLNSSLFEPTEIEHATLFISQLRNDESIPMYSSTVIKDDKGKKRTGTINPLEYLFEFL
                     NAYDFASEGSEEIQEDNKTLINASVLGLIFEKINGYKDGSFFTPGFITMYMCRETIRK
                     AVVQKFNEVKGWNCTDINS"
BASE COUNT          677 a          376 c          350 g          501 t
ORIGIN      
        1 acattttaca atgcctattc ctgtacaaaa aatgagcagc taaaatataa ccggagcaaa
       61 ccagatacat ttccggtgtt atatcattga taaacaaaaa acaccgtttc tccggatgat
      121 gtaaaattgt tcagtgtatc ggatagagac ggagcactgt gtcctactac tggtagatgc
      181 tccgtctcta caaaaagtca ggagattacg tccaaaccag ggagacagta aacaaaaaaa
      241 acaaaaactg attaattgtt gtaacacaaa ctgaaaattg tatatttgtg cataattata
      301 aaccggaaaa ctgattattg atacatggaa ctaaaaatct tgaagccacg gaaagcgctt
      361 aataaagctt ttctgaaagt aaaaccaagc aggactgaaa tcgagtgttt caagaccaat
      421 ctgtcacatc tgctcgacag tatcaatgac acgggatcag aagaatttca taagaacctt
      481 gtttccgatt ttctgaaaga tacgtattac aaacaaagcc attttgtaaa taccaaagga
      541 aattgtgacc ttgtaatcca caatgccgac aaagccacaa gtacagtcgg ggtaataatt
      601 gaagccaaaa agccaaccaa taaggctgaa atgcttacct tagacaagat aaacgtcaag
      661 gctttccagg aattggtgtt atactatctg cgtgaacgga ttactcagaa aaatattgaa
      721 attaaatacc tgattgcaac caatatttac gagtggttca tttttgatgt gacccagttc
      781 gacaggcttt ttgctcaaaa caaaaatctt gtaaaacaat tcatcgactt tgagagcggt
      841 cgattagccg atacgaaaac agattttttc tacaaacaaa tagctgagaa ttttattgcc
      901 gaaattaaac acgaagtaga atttacctat ttcgatattc gtgattacga aaaagcatta
      961 cgcaacgcag acaaaaagga tgataaccag tttattcccc tgtataagtt actctcgccc
     1021 caacatttgc tgaaacttcc ttttacaaac gacagcaaca gcctcgacaa acggttttat
     1081 agtgaattgc tccacattat cgggttaacc gaaaccaaag aaggcagcaa aaaactgatt
     1141 gaacgcaaca aggaaggtca acgcaattcg ggttcgctgc ttgaaaatgc aattatccaa
     1201 ctggacagcc tcgacaaaat atcaagaata gaaaacccgg gtcatttcgg ttccaacccg
     1261 caggaaaggc tttttaatgt cgggctcgaa ttgagtatta cgtggattaa ccggatttta
     1321 ttcctgaaac tgttagaagc acaattaaaa acgtaccata aaggcgataa atcctacgag
     1381 tttctgagtt ttagtaaaat caaagattac gatgatttaa acagtctgtt ttttcaggta
     1441 cttgcacgca gacagaacga acgaagcgac gatgtaaaaa cattattcgc taaagttcct
     1501 tatttgaaca gttcgctttt tgaacccacc gagattgaac atgccacact ttttataagt
     1561 cagttacgca atgatgaatc cattccgatg tattcatcta ccgtaataaa agacgataaa
     1621 ggaaaaaaac gaacaggtac catcaatccg cttgaatatc ttttcgagtt tctcaatgcg
     1681 tacgattttg caagtgaagg cagcgaagaa atacaggagg acaataaaac cctgatcaat
     1741 gcttcggtac ttggtctcat tttcgagaaa atcaacggct acaaagacgg ctcattcttt
     1801 acgcccggtt ttattaccat gtatatgtgc cgtgaaacca tccgcaaagc ggttgttcaa
     1861 aaattcaacg aagtcaaagg ctggaattgt accgacatca attc
//
LOCUS       DAOPYB010000362         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_91252_length_1900_cov_8.242818, whole genome shotgun sequence.
ACCESSION   DAOPYB010000362 DAOPYB010000000
VERSION     DAOPYB010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91252_length_1900_cov_8.242818"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..1068
                     /locus_tag="PKH58_14015"
     CDS             295..1068
                     /locus_tag="PKH58_14015"
                     /inference="COORDINATES: protein motif:HMM:NF022898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNX90187.1"
                     /translation="MVRGDNVNYSKFGKIMQGFPKSLVFLSSWLVSNGYSYELQQRYR
                     RSGWLKSIGKGAMLKTGDPFLLAGALAALQSQAKISIHLGGRSALELNGIAQYLQLAS
                     STSTLFAPGKTKLPFWFTNNKWDVDYQLFRTTLFNSETVGLVNYQEGGISMQISSPGR
                     AMLECLSLCPNKFSLTEAYELMEGISTLRPKQVQQLLEECKSVKTTRLFLYFAERIGH
                     SWYKYIDKTKINLGSGNRSLASNGVLIPEYNLVLPKELS"
     gene            1068..>1900
                     /locus_tag="PKH58_14020"
     CDS             1068..>1900
                     /locus_tag="PKH58_14020"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNX90188.1"
                     /translation="MKDAYKKQVALLLTILPEIAKEDVFALHGGTAINLFCLNMPRLS
                     VDIDLTYIPASENRERDLQNIRSGLERLKERLRKQIFKISFTDHVKAQEELKLICATP
                     EAVVKIEVNQINRGIIADTTLAVLCDKAQETFDKFCEVSMVSPKQLWGGKMVAALDRQ
                     HPRDLFDVRNLIHEIGYSSEIKEGFMFFLLCSKRPMHELLNPNLIDQSTVFKNQFDGM
                     TDHSFNFNDYKTTREELLRMINESLTPEDKEFLLAFSKGEPDWTNKDYSRYPAIRWML
                     M"
BASE COUNT          619 a          344 c          396 g          541 t
ORIGIN      
        1 aagcaacaga aagaggaaaa cggcgatgat gcttcgcata atgttgaaat tgaagcataa
       61 aaataggaaa aacaacggac ccattgcatt tcgcatgtca catttcgcat ttgatcaggc
      121 ccatcgcact tcgcacttca cagttcgcat tttatcaggt gcagagcacc gaaccatgtt
      181 tattttcgtg actgtattca tactttcagt atgattaata gcacctattt aaatttcatt
      241 tttgcacagc ttacaataac tatttatatt tgctcctgta tcaataccaa aagtatggta
      301 agaggagata atgtaaatta ttcgaagttt ggcaaaataa tgcaaggatt ccctaaaagt
      361 ctggtattcc tgtcttcctg gcttgtgtcc aacggatatt catacgaatt gcaacaacga
      421 tacaggagaa gtggatggct gaaatcaatc gggaagggag ctatgctgaa aacaggcgat
      481 ccgtttttgc tggcaggcgc tcttgctgct ttacagtctc aggcaaagat cagtattcat
      541 ttaggtggtc gttctgcatt ggagctcaac ggtattgctc aatatttgca acttgcctct
      601 tcaacatcga ccttattcgc tccaggtaaa acaaaattgc ctttttggtt taccaataac
      661 aaatgggatg ttgattatca actctttcga actaccttgt ttaattcaga aacggttggt
      721 ttagtaaatt atcaggaagg cggaatatcc atgcagatat caagtccggg acgagcgatg
      781 ttggaatgct tatcgttatg tcctaataaa ttttctttaa ccgaggcata tgagttaatg
      841 gaagggatat ctacattacg gccaaaacaa gtacagcaac tattagaaga atgcaagtct
      901 gtgaaaacaa caaggctctt tctctatttt gcagagcgga ttgggcatag ctggtataaa
      961 tacatagaca aaacaaaaat aaaccttggt tccggcaatc gcagtcttgc cagtaatgga
     1021 gtactgatac cggaatataa tttagtatta cccaaagagt tatcataatg aaagatgctt
     1081 ataagaagca agtcgcgctc ctgttaacta ttttgccgga aatagcgaaa gaggatgtat
     1141 ttgctcttca tggaggaaca gcgatcaatc tgttttgtct gaacatgcct cgcttgtcag
     1201 ttgatattga ccttacttat attccggcaa gcgaaaaccg agaaagagat ttgcaaaata
     1261 tacgatcggg acttgaaaga ttaaaagaac gattgagaaa acaaattttc aaaatcagtt
     1321 ttaccgatca tgtcaaagct caggaagaat tgaagctgat ctgtgctact ccggaagcag
     1381 tggttaaaat tgaagtaaac caaatcaatc gtggaataat tgcagacaca acattggcag
     1441 tactgtgtga taaagcgcag gaaacattcg ataaattctg cgaagtcagc atggtttctc
     1501 ctaagcagtt atggggaggt aaaatggttg cggcattaga caggcagcat cccagagatc
     1561 tatttgatgt caggaacctc atccatgaaa ttggctattc ttcagagatc aaagagggat
     1621 ttatgttttt cctcttatgc agcaaacgcc caatgcatga attgttgaat cccaatctaa
     1681 tcgatcagtc cacagtgttt aaaaatcaat tcgatggaat gacagatcat tcttttaatt
     1741 tcaatgatta taaaaccacc agagaagaac ttttaagaat gataaatgaa tcattaacac
     1801 ctgaagataa agaatttctg ctggctttct cgaaaggcga gccggattgg acaaataagg
     1861 attatagcag atatccggcg ataaggtgga tgttgatgaa
//
LOCUS       DAOPYB010000363         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_91481_length_1897_cov_9.179696, whole genome shotgun sequence.
ACCESSION   DAOPYB010000363 DAOPYB010000000
VERSION     DAOPYB010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91481_length_1897_cov_9.179696"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="PKH58_14025"
     CDS             complement(<1..1549)
                     /locus_tag="PKH58_14025"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HNX90189.1"
                     /translation="MKTLKISLLLSCIVLLSHPTMLLAQKGNGNVVTQDRQVAQFSAI
                     EAGSAVNVFLSQGAAQSVKVEIDENLLDRFETDVKNGVLTINVSGVNNATKMNAYVTM
                     VTLKRIEANGAANINGQTPLASDNFTYEGTGASNATFEIKAGDFSLTLSGASKANINA
                     GVNTLKTEVSGAGSLTLSGSAASHNLEVSGAGNVKAMELVTDVTNAEVSGAGNAKVMA
                     KQQLKSDVSGAGHLSYFDNGQMREISAPDEKTRSLSGMENIKSVIIEDENGEKQVLFE
                     TKEGEDTLSVKVGPNKVVVITDDTVRVKLGNNEVVVNDEGDVKVKHDKKKKFNGHWTG
                     LELGINGYLTPDMDFNYPSAYSGLEQNYVKSTNVNLNLFEQNFNLIKNKLGLVTGVGF
                     TWNNYRFDATETRMGKGDDGKIAVYPDPNTDIRYIKSKLVVNYLTVPLMLEYQTNPRS
                     KSNSFHLGAGVVGGLRIGSHTKYVYDDGGKQKDKNPGDFYLNPFKVDGIVKIGWGVIN
                     LYGTYSFS"
BASE COUNT          483 a          402 c          382 g          630 t
ORIGIN      
        1 ttgagaatga gtatgttccg taaagattta ttacacccca gccaattttc acaataccat
       61 ctaccttgaa tgggttcaga tagaagtcgc cgggattttt atctttctgt ttgcctccgt
      121 catcataaac atactttgtg tgtgagccga tgcgaagacc tccgactaca cctgctccca
      181 ggtgaaaact gttggatttt gaacgcggat tagtttggta ttcaagcatc aggggaacgg
      241 tgaggtagtt tacgaccaac ttgcttttga tgtatctgat atctgtattt ggatcagggt
      301 aaactgctat ctttccgtcg tcgccctttc ccattcttgt ttcggtggca tcaaagcggt
      361 aattgttcca tgtaaaacct acaccggtta caagcccgag tttattttta ataagattga
      421 aattctgttc aaagaggttc aggtttacgt tggtagattt cacatagttc tgttcaaggc
      481 ctgaataggc tgacgggtaa ttgaagtcca tatcgggagt aaggtaacca ttaataccaa
      541 gttccagccc ggtccagtgg ccgttgaatt ttttcttctt atcgtgtttt actttcacat
      601 cgccttcgtc gttcacaact acttcattgt taccaagctt aaccctgaca gtatcatcag
      661 tgattacaac aaccttattt ggcccaacct ttacacttaa agtgtcttca ccttctttgg
      721 tttcgaaaag aacttgtttt tcaccgtttt catcttcaat gatcacactt ttaatgtttt
      781 ccatgcctga gagtgacctg gttttttcgt cgggggcgct tatttctctc atttggccat
      841 tgtcgaaata tgacaggtga ccggcacctg agacatcaga cttgagttgc tgcttagcca
      901 tcacttttgc attgccggct cccgaaacct cagcatttgt aacatcggtt acaagttcca
      961 ttgctttaac attgccggcg ccgcttactt caaggttgtg acttgccgct gagcctgaaa
     1021 gtgtaaggct tccagcgccg ctcacttcag tttttaatgt atttacacca gcattaatat
     1081 tggccttcga tgcgccgctc agggtcaaag aaaaatcgcc ggctttaatc tcgaaggttg
     1141 cattagatgc accagttcct tcataagtaa agttatccga tgccagggga gtttgcccgt
     1201 tgatatttgc tgcgccattg gcttcaattc gtttcagtgt caccatagtc acataggcat
     1261 tcattttggt agcgttgttc acaccgctga cattgatagt aaggacgccg tttttgacat
     1321 ctgtttcgaa acggtcaagg aggttttcat caatttcaac ttttaccgac tgtgcagcac
     1381 cctgggataa aaatacgttt acagccgatc cggcttcaat agcgctgaat tgggcaacct
     1441 ggcgatcctg tgtaactaca ttgccatttc ctttttgtgc aagcagcatt gttggatggc
     1501 tcaacaggac tatgcagctt aacaagagac tgattttcag agttttcata gtgataggtg
     1561 tattatgttt tgttgatttg ctgttaattt ccgaatgtca gacggactca gcaaatgatt
     1621 tgttacagcc agagtatctt tttttaaaat atctgcagac atgaaagacg attcgaccgt
     1681 tggttaacgt taccgaaatt atttcgggcc gtcaggatgc ttaacttctt tcatacattt
     1741 aatgaagaac agagaattgt tttgaaatac aattgtaaac gtcataaaat caggacattg
     1801 atattaaatg aattttaaaa tcggtattcc ttgctttttt acttttaaag gttatttttg
     1861 caaaaaatta caacctaaca atcttaccat ataatgt
//
LOCUS       DAOPYB010000364         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_91917_length_1891_cov_8.344227, whole genome shotgun sequence.
ACCESSION   DAOPYB010000364 DAOPYB010000000
VERSION     DAOPYB010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91917_length_1891_cov_8.344227"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1891
                     /locus_tag="PKH58_14030"
     CDS             <1..>1891
                     /locus_tag="PKH58_14030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX90190.1"
                     /translation="VNVTMTSDSKLLSEVVVTGYTSEKKADITGAVSVVKMKDILSIP
                     TGNAISSLVGRLPGVNVYTDGTPGGVGTNTTIRGITSNGNISPLLVIDGVQTRENLST
                     LLNANDIESIQVLKDAASASIYGVQAANGVIIITTKKAVKDKIKVDFDAQFTAQNYHS
                     NIKMLNAQQWGDTYWQAYLNDGQKPNHDQYGNGAQPVIPEFINQQKTIRSGDTDWAKQ
                     VYKTSFQQNYNLNVSKGTNNGSSTFSMNYFNQDGLIKYTNFTRLNTRFNSTYSFFENK
                     LRIGENVTISNWNQIVKPGGIEELTIAQHPLIPVYDINGGYAGPTQGLGDKPNPIRLL
                     DQQRENKDKSWRIFGNAFIEIEPVKNLVFRSNFGLNYRTQFWSAFQPKWAEGDRIVDK
                     NSLSTSNNYSQEWIWSNTVAYGLQLQKNSFNFLLGQESKESVNEFVTGTRENFLIQNL
                     DYRYLDAGGGKQTNAGNADRYSIVSYFGKMNYAYNDRYLFSATLRRDATSRFGTNNNS
                     ALFPAFTAGWRISQEEFMKDVDFVSDLKLRASWGVNGNDNADNEATYTKYAINAILAG
                     YDISGANTGTIASGIYKVRSGNPNIKWETTTQKNIGLDAAFLANRLNLTLDYYMKDTR
                     DMLIER"
BASE COUNT          640 a          341 c          379 g          531 t
ORIGIN      
        1 cagtgaatgt aacgatgaca tctgattcca agttattaag tgaagtcgtt gttacaggtt
       61 atacctcaga aaagaaagca gatattaccg gtgctgtttc agttgttaaa atgaaagata
      121 ttctaagtat tccgacaggg aatgcaattt ccagcctggt aggacggtta cccggtgtaa
      181 atgtatatac agatggtaca ccaggtggtg ttggaactaa tacaactatc aggggaatca
      241 cttcgaatgg aaatatctca cccttgttag taatagatgg agtgcaaaca cgtgaaaact
      301 tatccacttt actaaatgca aacgatatag agtcaattca ggtattgaaa gacgctgctt
      361 ctgcatcaat ttacggggtt caggctgcga atggtgttat aatcattacc accaaaaagg
      421 cagttaaaga taaaataaaa gtagattttg acgcacaatt tacagcacaa aactatcatt
      481 caaacattaa aatgctgaat gcacaacaat ggggtgatac atactggcaa gcttatctta
      541 acgacggtca aaagccaaat catgatcagt atgggaatgg ggctcagccg gttattcctg
      601 aattcattaa tcagcagaaa accattcgtt cgggtgatac tgactgggca aaacaagttt
      661 ataaaacttc ttttcagcag aactataact taaacgtttc aaaaggcaca aataatggat
      721 cgtctacttt ttcaatgaat tatttcaatc aggatggatt aataaaatac accaatttta
      781 cccgattaaa tacccgtttc aattcaacct atagtttctt tgaaaataaa ttaagaattg
      841 gtgaaaacgt aactattagc aactggaacc aaatagttaa accgggtggt attgaagagt
      901 taacaatagc tcaacatccg ttaattcctg tttatgatat aaatggtgga tatgccggtc
      961 caactcaggg cttgggagat aaacctaatc ctatccgatt acttgatcag caacgggaaa
     1021 ataaagataa aagctggcgt attttcggta atgccttcat tgaaattgaa ccggtgaaaa
     1081 atctggtgtt ccgttccaat tttggtttga attatcgtac gcaattttgg tctgcatttc
     1141 agccaaaatg ggcagaaggt gaccgtattg tggacaaaaa cagcttgtca acctccaata
     1201 attacagcca ggaatggata tggtccaaca cagtagcata tggtttacaa ttacaaaaga
     1261 attcattcaa tttcttacta ggacaagaat ccaaggaatc tgtcaatgaa tttgtaacag
     1321 gaacacgtga gaatttctta atacaaaacc tggattatcg ctacctggat gctggtggag
     1381 gcaaacaaac caacgccgga aatgctgacc gttattcgat tgtttcctat ttcggaaaaa
     1441 tgaattacgc atacaatgac cgctatttgt tctcagccac acttcgcagg gatgctacat
     1501 cgcgtttcgg aactaataat aattcggcac tttttcccgc gtttacagcc ggttggagaa
     1561 ttagtcagga agaatttatg aaagatgttg attttgtttc tgacttaaaa ctgagagcaa
     1621 gctggggtgt aaacggtaat gataatgccg acaatgaagc cacttacacg aaatatgcaa
     1681 tcaacgccat tttagcaggt tacgatattt ctggagcgaa tacaggtact attgcaagtg
     1741 gcatttacaa agttagatca ggtaatccga acataaaatg ggaaacaact actcagaaaa
     1801 atatcggttt agatgctgca ttccttgcca acagacttaa tctgacactt gattattata
     1861 tgaaagacac ccgcgatatg ttgattgagc g
//
LOCUS       DAOPYB010000365         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_96245_length_1836_cov_10.143739, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000365 DAOPYB010000000
VERSION     DAOPYB010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96245_length_1836_cov_10.143739"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKH58_14035"
     CDS             complement(<1..463)
                     /locus_tag="PKH58_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90191.1"
                     /translation="MKKFVYLLCMGILFSMQVSAQGWLDKVGQKAVNTAKQKVENRAA
                     KKAGEATDKALDKLEKKDNKKVETEENEEKSVQTADKTVKEEKLTATSKYDFIPGEKV
                     IFYDDFSQDAVGDFPALWNTNGSAEVVTTNLFPGNWMKLNSECCVWTDKLLT"
     gene            complement(977..>1836)
                     /locus_tag="PKH58_14040"
     CDS             complement(977..>1836)
                     /locus_tag="PKH58_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNX90192.1"
                     /translation="YVMSLMNGARLGVGAQSVGVSEAAYQEAVAYANDRKQFGKAIVE
                     FPAVYEMISLMKAKLDASRALLYETTRFVDVYKAYEGIEGERKLTPEEKADYKEYQKL
                     ADAFTPLVKMFASEYANQNAYDSVQVHGGSGFMKDYACERIYRDARILTIYEGTSQLQ
                     VVSAIRHVTTGNYLKQIREYEKQTINPEFHSFRKRLMVMTNLLENSISRVDEIKNQEY
                     LDFQARRLVEMAGHIIMGYLLLIDGSRNEMFRKSAEVYLNYSEVEVRKHAAFIKTFNV
                     ESLDRYRMA"
BASE COUNT          485 a          396 c          333 g          622 t
ORIGIN      
        1 gagttaaaag tttgtcagtc catacacagc attccgagtt aagtttcatc cagttgcccg
       61 gaaacaaatt ggttgtaacc acttctgctg agccgttggt attccagagt gccgggaaat
      121 cgcccacagc gtcctgactg aaatcatcgt aaaaaattac tttttcaccg ggaataaaat
      181 catacttcga agtagccgtc agtttctctt ctttcacagt tttatcggct gtctgtacac
      241 ttttttcctc attctcttcg gtttctactt tcttattgtc ctttttttcc agtttatcaa
      301 gcgctttgtc ggttgcttcg ccagcttttt tggcagcgcg gttctctact ttttgcttag
      361 cggtatttac agctttttga cctactttat cgagccaacc ctgagcactt acctgcatac
      421 tgaataaaat gcccatacat agtaaataaa caaacttttt catggtacaa ttctttttta
      481 tgattataaa aacagtaaat tattctgtga atgctgataa gaaatggagg tataaataac
      541 tttcaataag tttttaaata attttttctt attgttttat tttttatata acacactgaa
      601 gttctgtaat gtttatcgga ttttttgaca ttatttatta aggttgaatt tccctgtttt
      661 tttactattt tatttactgt tgtccgttaa aaatatttag tcccatcgct tcgctctgga
      721 cgacacgtaa ttatagtgat ttatgaaagg atggggttgg ttggcggctt cgccgccaac
      781 caaccccatc cttaaagaca aaatactgac tgtcatttcg aacgaagtga gaaatctaat
      841 atatttcaag aatttataat tttaccggac aacagtgtat tttattatta attcccgcga
      901 aagtcatcat taccgtacaa aaaaaaacgc tgaaatcatc agaaatttca gcgttttctt
      961 gtttgaaact agtattttaa gccattctgt atctgtccag actttccaca ttgaaagttt
     1021 taataaatgc agcatgttta cgtacttcca cttcggagta attcagataa acttcggccg
     1081 atttacggaa catttcgttg cggctgccat caatcagaag taaataaccc atgatgatat
     1141 gacctgccat ttccaccaga cgacgagcct ggaaatcgag gtattcctga tttttgatct
     1201 cgtcaacacg acttatcgaa ttttccagca gattggtcat caccattaat cttttgcgga
     1261 acgaatgaaa ttcgggatta atcgtctgtt tttcgtattc tctgatctgc ttcaggtaat
     1321 taccggttgt gacgtggcgg atggctgaaa ctacctgcaa ctgtgatgtt ccttcatata
     1381 tggtaaggat acgtgcatcg cgataaatac gctcacaggc ataatctttc ataaagcccg
     1441 agcctccgtg aacctggacg ctgtcgtatg cgttttggtt ggcatattcg ctggcaaaca
     1501 tctttacaag cggagtaaac gcgtcggcca gtttctggta ttccttgtaa tcggcttttt
     1561 cttcgggagt gagtttgcgt tcgccttcaa taccttcgta ggctttatat acatcaacaa
     1621 aacgggttgt ttcataaagc agtgctctgg acgcatccag cttggctttc atcagcgaaa
     1681 tcatttcata taccgccgga aactcaacaa ttgcctttcc gaactgctta cgatcatttg
     1741 cataagctac ggcttcctgg tatgctgctt ccgatacacc aactgactgt gcgcctacac
     1801 caagccgtgc tccgttcatc agcgacatca catact
//
LOCUS       DAOPYB010000366         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_97829_length_1818_cov_7.475326, whole genome shotgun sequence.
ACCESSION   DAOPYB010000366 DAOPYB010000000
VERSION     DAOPYB010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97829_length_1818_cov_7.475326"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PKH58_14045"
     CDS             complement(<1..740)
                     /locus_tag="PKH58_14045"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HNX90193.1"
                     /translation="MKNRALKTIKDNLEEEIIRDLDRIGVYYRIHSRTKTLESLQQKI
                     LTKGEGYYRTEGKKVQDMIGFRITTYFLDDVNLLWNYFSMKYEIVDKQYDLPTSDVFK
                     PLRKNLICRFSADNSAIFDGAKLSGHELELVDSTYEVQFRTTSSEGWHEVDHALRYKC
                     KNDWVPYRDEDKMFNGIYATLETTDSAMKSLFEDMTYNHYKNKNWEAMLRMKFFLKFD
                     NEELSEPVKKLLNTNHELAKNIFKCSRA"
     gene            complement(975..>1818)
                     /locus_tag="PKH58_14050"
     CDS             complement(975..>1818)
                     /locus_tag="PKH58_14050"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HNX90194.1"
                     /translation="FVMKKVFFSLIAFAVMLTACNSNDMDEAASGKRNVSFFLTDAPG
                     NADHFGKGHNPWTRFSGVNLDIAGIQYMAVDTIKKDSTIWTDVPFNQQTIAVSLLSNG
                     DSVFLSDFNIPEDQIVRKIKFKLGKNSTVILSDSTTKPLLISDKSDSTLIIHVRNNVP
                     KGKYSIMLDFDIAHSIIPGKDGNFYLKPRMRCFVRETTASIGGFVMPLRLATKVFVVI
                     DNDTITTVSDVNRFNLFKLDGFTTGTYTLQFMPVDSARVTFTKTVEIKKNKDVFLGKV
                     KVIN"
BASE COUNT          481 a          387 c          370 g          580 t
ORIGIN      
        1 tcagcacggc tgcatttgaa aatgtttttt gccagctcat gatttgtatt aagcagcttt
       61 ttgaccggtt cactcaattc ttcattgtcg aatttcagga agaatttcat tcgcagcatg
      121 gcttcccagt ttttattttt gtaatgattg tatgtcatat cctcaaacag tgacttcatc
      181 gcgctgtcgg tagtctcaag ggtggcgtat atcccgttaa acattttatc ctcgtcgcgg
      241 taaggaaccc agtcgttttt gcacttgtac ctcaacgcgt gatccacttc gtgccaccct
      301 tcggatgaag tggttcgaaa ctgaacttcg taggtgctgt caaccagctc cagttcatgg
      361 ccggataatt ttgccccgtc gaaaatcgca gaattgtcgg ccgagaaccg gcagatcaaa
      421 ttctttcgga gtggtttgaa gacatcagat gttggtaaat catattgctt atctacaatt
      481 tcatatttca tcgaaaaata attccacagc aggttaacat cgtccagaaa gtaagttgta
      541 atacggaaac ctatcatatc ctgcactttt ttcccctctg tccggtagta gccttcgcct
      601 ttagtcagta tcttttgttg taacgattcc agtgttttgg ttctggaatg tatccggtaa
      661 tacactccaa ttcggtcaag atcacgaatg atttcttcct caaggttgtc ttttatcgtc
      721 ttcaatgctc tgtttttcat ttgaaaatca acgtcatttt aattgtcaaa tgtacgctaa
      781 aaacacacgg ttatcaatgc agaatcggtt aaacggtata atcagaaaac acattattga
      841 ttgcacccgc gaatataaaa cagattgact tagtattatg tttcagaatg acgtatttct
      901 ttcaataaat ggatttaaaa cacagaaagg cacaaaatga attgtacctt tctgtgtctg
      961 ttctcttata cttatcagtt aatcactttc acttttccca ggaaaacatc cttgtttttc
     1021 ttaatctcca ctgtctttgt aaaagtaact cttgcactgt ccacaggcat gaactgtagc
     1081 gtgtaggtgc cggttgtaaa gccatcaagt ttaaaaagat tgaacctatt aacgtccgaa
     1141 acggtggtta ttgtgtcgtt gtcaatgacc acaaacactt tggtggcaag tctcaacggc
     1201 ataacaaaac ctccgatgct tgccgttgtt tcccttacaa aacaccgcat cctgggtttc
     1261 aggtagaaat taccgtcctt gcccggaata atcgaatggg caatgtcgaa atcgagcatg
     1321 atggagtatt tacctttcgg aacattgttg cggacgtgaa tgatcagcgt actgtcgctt
     1381 ttgtcggata tcagcaacgg ttttgttgtg ctgtccgaca ggatgactgt gctgtttttg
     1441 cccagtttga atttaatctt ccgtacaatt tgatcttcgg ggatgttgaa gtccgaaaga
     1501 aatacgctgt cgccgttgga aagcaaactt acagcaatag tctgttggtt aaacggaacg
     1561 tccgtccaga tagtcgagtc ttttttaatg gtatctacag ccatgtattg aattcccgcg
     1621 atatccagat taactcctga aaaccttgtc caggggttgt gcccttttcc gaaatgatcg
     1681 gcgttgccgg gtgcgtcagt cagaaagaac gatacattgc gtttgccgct agctgcttca
     1741 tccatgtcgt tgctgttgca ggcagtgagc ataacggcga aagctatcaa agaaaaaaat
     1801 acttttttca taacgaat
//
LOCUS       DAOPYB010000367         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_98005_length_1816_cov_6.541170, whole genome shotgun sequence.
ACCESSION   DAOPYB010000367 DAOPYB010000000
VERSION     DAOPYB010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98005_length_1816_cov_6.541170"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /gene="udk"
                     /locus_tag="PKH58_14055"
     CDS             complement(<1..421)
                     /gene="udk"
                     /locus_tag="PKH58_14055"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNX90195.1"
                     /translation="MLIIGIAGGTGSGKTTVVRKIVERLPVGEVVILPQDSYYKDSGH
                     LPLEERLEINFDHPDSIEFDLLTKHLKELRKGHSVQQPKYSYLTCTRAAETITVNPCD
                     VVIVEGILVLCNPELRKMMDLKVFVDADPDDRLIRVIN"
     gene            715..1797
                     /locus_tag="PKH58_14060"
     CDS             715..1797
                     /locus_tag="PKH58_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNX90196.1"
                     /translation="MSSIYSVITGSGSYVPPKIVKNEDFINNEFCDTNGELLTTPTSQ
                     IVSKFMEITTISERRYADDNQTTSDLALIASQKAIKAAGIDKEQLDYIIFAHNFGETT
                     HNNINTDIVPALAARLKQKLGVKNPNTVAYDLLFGCPGWLQGVIQADYYIKSGDAHKV
                     LVVGAEVMSRISDPHDRDSMIYADGAGAVVLEGRESDTPVGILGHNARSDSYLYAQML
                     HMGASYNPERPDPKDLFIKMNGRRLYMYALDNVPKAIKAGLDKLGLHINQIQKLLIHQ
                     ANGKMDDAILKRLFELYEINEVPENVMPMTIAKLGNSAVATLPTLYDFVINKQIEGHE
                     LNSGDLAVFASVGAGMSINSFVYQMP"
BASE COUNT          570 a          359 c          384 g          503 t
ORIGIN      
        1 ggttgatgac gcgtatgagg cggtcgtcgg ggtcggcatc cacaaacact ttgaggtcca
       61 tcattttgcg cagttccgga ttacacaata cgagaatccc ttccactatg accacatcac
      121 aggggttgac ggttattgtt tcagcagcac gtgtacacgt gagataggag tattttggct
      181 gttgtaccga atgtcctttc cttagttcct tcaaatgttt tgtcaatagg tcgaattcaa
      241 tggagtcggg atggtcgaaa ttaatctcca gtctttcttc gagtggcaaa tgaccgctgt
      301 ccttgtaata cgaatcctgg ggtaaaataa ccacctcgcc tacaggaagg cgttctacta
      361 ttttgcgtac tacggttgtt ttacccgaac ctgttccacc tgcaattcca attattaaca
      421 tgagttttgt tttatgattt tcagtgcact aaaatagtaa atattgattg aattatttgc
      481 attggtgcta aaaaaaatga aaattgtaaa tttcagcagg attattagct ggggaaaaag
      541 atacgataca tggatgtttt ccatgaagaa aacgaaataa taatgggtga tatccggtat
      601 tagaccttga aatattctaa aaaaataaag aatgttttta acttgatggg taaaattggg
      661 taagaatgtt tacctttgcg aaaattttat atccttaatt gaaaatatta atctatgagt
      721 tcaatataca gcgtaattac cggttcaggc agttatgtac ctcccaagat agtaaaaaat
      781 gaagatttca ttaacaatga attctgtgat actaacggcg aattactgac cactcccacg
      841 agccagattg tcagtaaatt catggaaatt accaccatat ccgaacgacg atatgccgat
      901 gataatcaaa caacctcaga ccttgcactg atagcatcac aaaaagccat caaagcagcc
      961 ggaattgaca aggagcaact cgattatatc atttttgctc ataattttgg tgaaaccaca
     1021 cacaacaata ttaatacaga tattgtacct gcgttagcag cacgcttgaa acaaaaactg
     1081 ggagtaaaaa atcccaatac agtggcatac gatttgctat tcggatgccc gggatggttg
     1141 caaggtgtta ttcaggccga ttattacatc aaatcgggcg atgctcacaa agtgctggta
     1201 gttggcgctg aagtaatgtc gcgaatatct gaccctcacg atcgcgacag tatgatctat
     1261 gccgatggag ccggagctgt agtgcttgaa ggccgtgaaa gcgatacccc tgttgggatt
     1321 ttaggtcaca acgcccgcag cgactcctat ttgtatgcac agatgcttca catgggggct
     1381 tcgtacaatc ctgaacgacc tgatccaaaa gatctgttta ttaaaatgaa cggacgcaga
     1441 ctttatatgt atgcgttgga taatgtaccc aaagcaatca aagccggact agacaagctg
     1501 ggactgcata tcaaccagat acaaaaattg ctgatccacc aggcaaatgg taagatggat
     1561 gatgccatcc tgaaacgcct cttcgaactt tatgaaatca acgaagtgcc cgaaaacgtc
     1621 atgccaatga ctattgccaa acttggaaat tcggccgttg caactctacc aacgttgtac
     1681 gattttgtaa taaataagca aatcgaaggt catgaactta acagtggcga tttagcagtg
     1741 tttgcatcgg taggcgccgg aatgagcatc aatagttttg tgtaccaaat gccataagat
     1801 cagaaagaat aaattt
//
LOCUS       DAOPYB010000368         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_98247_length_1813_cov_8.457338, whole genome shotgun sequence.
ACCESSION   DAOPYB010000368 DAOPYB010000000
VERSION     DAOPYB010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98247_length_1813_cov_8.457338"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="PKH58_14065"
     CDS             <1..1485
                     /locus_tag="PKH58_14065"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90197.1"
                     /translation="NILLDDVAKARLVKELDCIISGGLQEHFMINCKIAAICDRLKIL
                     RAPAKDATASWFINYCLGICGQNPITENYHYEQFLYYRSKYFNSSVNFEPFRFEVPLE
                     YSQLIMNELEKELGDEYTFYIIAKPPNQYNIESDIVEYNGLKYYYDYEDMAVVERVIL
                     HRQEECYFITERIDGKNFVFFNEPESDYKRHSPYIYTIWENMELGFIDDVVKKLPEKQ
                     HPYLIEMDNQDVFSFIASGKNPFMMYPCRIFVPDHIQIFNPKSIQELMVFIEQNFSDI
                     PSPSHFHHNKEYSAFCSKLNYTNIFKNDKHVSEILAISYDEIITLDQVYRLAVEVGGM
                     KPNDFFELLSKIPSREDFRIEVVIEELSNNIAREILENSSLDSTEIEKLRKKIEIRLY
                     DTRYPHMHLSGLYHAAVISYWLAYYYYLFPQIAKEPFDEYYKTLEKLTNEHVIDDSFY
                     EMRDSVNKSNERKYEITFLEFVEALKKDEDYDGDIAEKIRKELR"
     gene            1615..>1813
                     /locus_tag="PKH58_14070"
     CDS             1615..>1813
                     /locus_tag="PKH58_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90198.1"
                     /translation="MLFNPTQKQLLNLILEYEKPQAPDDLILTYQDFMKVRDFLSYYP
                     FNPVVFENLLRLTIDLWTSDTR"
BASE COUNT          621 a          278 c          344 g          570 t
ORIGIN      
        1 aatattcttt tggatgatgt tgctaaagcc cgtttagtaa aagaactgga ctgcattata
       61 agtggaggat tgcaggagca ttttatgata aattgtaaaa tagctgcaat ttgcgacaga
      121 ctgaaaattt taagagcccc ggcaaaggat gcaactgcaa gttggtttat aaattattgt
      181 cttggcattt gcgggcaaaa tccaattacg gaaaattacc attacgagca atttttatat
      241 tacagatcca agtatttcaa ctcatcagtg aattttgaac ctttccgctt cgaagttcct
      301 ctcgaatatt cacagttgat aatgaatgaa ctggaaaaag aacttggaga tgaatatact
      361 ttttacatca ttgctaaacc tccgaatcag tacaatatcg aaagtgatat tgttgagtat
      421 aatggcttaa agtattatta cgattatgaa gacatggctg tagttgaaag ggtaatttta
      481 cacaggcagg aagaatgtta tttcataacg gaaagaattg acggaaagaa ttttgtgttc
      541 tttaatgaac cggaatctga ctataaaaga cattctcctt acatatacac catttgggaa
      601 aatatggagt tgggttttat cgatgacgtt gtcaaaaaat tacccgaaaa acagcatcct
      661 tacctgatag aaatggataa tcaggatgtt ttcagtttta tagcaagtgg aaaaaatcca
      721 tttatgatgt atccatgcag aatattcgtt ccggatcaca ttcagatttt taatccgaaa
      781 tccattcagg aattgatggt ttttatcgaa cagaatttct cagatatccc cagtccaagt
      841 cattttcacc ataacaagga gtattcagca ttctgcagta aactgaacta tacgaatatt
      901 ttcaaaaatg ataagcatgt aagcgaaatt ctggcaataa gttatgatga aataatcacg
      961 ttagatcagg tgtatagatt agctgttgaa gtcgggggaa tgaaacccaa cgattttttt
     1021 gagttgttat ctaaaattcc ttcgagggag gattttagaa tagaagtggt aattgaagaa
     1081 ttgagtaaca atattgcccg ggagatactg gaaaacagca gtcttgactc aacggaaatt
     1141 gaaaaattaa ggaaaaaaat agaaatcagg ctttatgaca cccgatatcc gcatatgcat
     1201 ttatcgggac tttatcatgc agcagtaatt tcctattggt tagcttatta ttactatctg
     1261 tttcctcaga tagcaaaaga accttttgat gagtattata aaacgttaga aaaactgacg
     1321 aatgagcatg taattgatga ttctttttac gagatgcggg attctgtcaa taaatcaaac
     1381 gaaagaaagt atgaaattac ttttttagag tttgttgaag ccttgaagaa agatgaagat
     1441 tacgacgggg atattgctga aaaaattcgg aaagagttac gataaagttg gttttcgctc
     1501 tctttctccc ggcaataaaa gaataatcag ctgtttttac ggaaaataaa aactcctgat
     1561 ttgaattaat atttattaga taaaacacaa acttacctaa ctgccttacc tctcatgctt
     1621 tttaatccta ctcaaaaaca attattaaat ctaatcctgg aatatgaaaa gcctcaggct
     1681 cccgatgatt taatcctgac atatcaggat tttatgaagg tccgggattt tctttcttat
     1741 tatccgttta atccggtagt gtttgaaaat ttgctaaggt tgactatcga tttgtggaca
     1801 agtgacacgc gaa
//
LOCUS       DAOPYB010000369         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_100357_length_1789_cov_8.163783, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000369 DAOPYB010000000
VERSION     DAOPYB010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100357_length_1789_cov_8.163783"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..481)
                     /locus_tag="PKH58_14075"
     CDS             complement(149..481)
                     /locus_tag="PKH58_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNX90199.1"
                     /translation="MKEIKAFVKPFKVNDILNQLLQAGYPNITASMAEGTGNFASDES
                     TLSTHFSITDSKVAKIEIVCNDSEVENIVNIISTKGRTGNPGDGIIYVSEVEKVYKVR
                     TGLENGED"
     gene            complement(505..1689)
                     /locus_tag="PKH58_14080"
     CDS             complement(505..1689)
                     /locus_tag="PKH58_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX90200.1"
                     /translation="MTKYKFISPIIILAALLAFSSCKGDKKQGVEAKETEVIPENIVE
                     LSGDQVKLANLETGAIELRSLSGTLKVSGVVAAAPQNMAMVSMPMGGFVKSTSLMPGN
                     SVSKGQTLAVIENSEFIDIQQNYLEAKSKLEYAEADYNRQNELYKSDVSSKKSMQLVT
                     SEYRSLKVQVKSLEQKLSILGINPNKLNENNISKSITLVSPISGYVKTVNVSIGKSVS
                     ASDVLFEIVNTSKLFLQLTLFEKDADKVSTGQKIRFFINNETEQHMALIYQTAKSVDA
                     DKTYKVYASVQGTCKNVLPGMYVNAVIEATTSKVTAVPSEAIVSFEDKDYIFVFERNK
                     KESGKPFTEYRMIQVKKGVTDEGYTEITLPDNYNFKTEKVIVKGAYNLLSAKKNAGEM
                     AC"
BASE COUNT          471 a          350 c          315 g          653 t
ORIGIN      
        1 caatcttaga agcgcaggaa gcgcaatcta agttattcaa tttgtatttt ttcattttta
       61 gagtgtcttg tttatttgca gaaaaataat tattagtcag gatttgttaa atattgcccg
      121 gtttaaccgg gcaatattta catggtaatt aatcttcgcc gttttctaag ccagttctca
      181 ctttgtacac tttttctact tcggagacat agataattcc atcacccgga tttcctgttc
      241 ttcctttagt ggaaattatg ttcacaatat tttctacctc actatcatta cagacaattt
      301 caattttggc cactttacta tccgttattg aaaaatgagt tgaaagtgtt gattcatcgc
      361 tcgcaaaatt tcctgttcct tcggccattg aggctgttat gtttgggtaa cctgcctgaa
      421 gtaattgatt taaaatgtca tttactttaa aaggctttac aaatgctttt atttccttca
      481 tgattgtttc tttttagagg ttgattagca cgccatttca cctgcatttt tctttgccga
      541 aagcaagtta tatgctcctt ttacaatgac tttttcagtt ttaaaattgt agttgtcggg
      601 caatgtaatt tcggtatatc cttcatccgt cacccctttt tttacctgta tcattcggta
      661 ttctgtaaaa ggtttgccgc tttctttttt gttccgttca aaaacaaaaa tatagtcttt
      721 gtcttcgaaa ctcacaattg cctcggaagg cacagcagtt actttgctgg tagtggcttc
      781 aattaccgca tttacataca tacccggcaa tacatttttg catgtcccct gaacacttgc
      841 atacacctta taggttttat cggcatcaac ggatttggcg gtttggtaaa tcagtgccat
      901 gtgctgttcg gtttcattat tgataaaaaa gcgtattttt tgaccggtag aaaccttgtc
      961 agcatctttt tcaaacagcg taagttgaag gaaaagtttg ctggtattta caatttcaaa
     1021 tagcacatcg gaagccgaaa ccgatttgcc gatacttaca tttaccgttt ttacatatcc
     1081 ggaaattggt gaaaccaatg taatggattt actgatattg ttttcgttca atttattggg
     1141 attgatacct aagatagaca acttttgttc cagtgatttt acctgcactt tcagacttct
     1201 atactcggac gtaactaatt gcatactctt ctttgacgaa acatcactct tgtatagctc
     1261 gttttgtcgg ttataatccg cctcggcata ttcaagtttg ctttttgctt cgagatagtt
     1321 ttgttgaata tcaataaatt cggagttttc aataacagcc aacgtttggc cttttgagac
     1381 tgaattaccc ggcataaggg acgtactttt cacaaagcca cccattggca tgcttaccat
     1441 agccatgttt tgaggtgcag cggcaactac accgctcact ttgagggttc cgcttaggga
     1501 acgcaattca attgcacccg tttctaagtt ggcaagtttt acctgatcgc cactcagttc
     1561 cacaatattt tcgggtataa cttctgtttc tttggcttct acaccctgtt ttttatctcc
     1621 tttgcatgac gaaaatgcga gaagagcagc tagaataatg ataggtgata taaatttata
     1681 ttttgtcata atgattgttt tttagacccc caacccccaa agggggctaa aggtgtgaaa
     1741 ttaatatatt tttccggtaa taaagtctat tgaaataacg gtttgatta
//
LOCUS       DAOPYB010000370         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_101390_length_1777_cov_7.238095, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000370 DAOPYB010000000
VERSION     DAOPYB010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101390_length_1777_cov_7.238095"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1768)
                     /locus_tag="PKH58_14085"
     CDS             complement(<1..1768)
                     /locus_tag="PKH58_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90201.1"
                     /translation="MKGTGYSGYMRWKEEFSHIIGPSGSMKEAQSKVLDFYESFNDIK
                     NRMPSVNGNWTELGPKFLPTNIDSLKNYAGIGRIHAVAFNPNNANHVFAMSPTGGLFY
                     SLDGGNTWNNGHTDSMKIVGVSYLVVDPQNSSRWYIGTGDCDGASVNWCMNTSDGVYR
                     TEDMGVTWVQIFDTSLFYRPNRVRKLLMHPTNNNILFTVTDTAIYKTTNATAANPIWN
                     RVCSGSFNDLHYRPGNNDTMYASGYYGSKLLVSTNGGDSFSLIPNTPFRDTTNIMIEF
                     SPANPDLLFITECYDTIQLHILNLYTNQWISRGWVKYLENNYTNYLYNIAKGFAVSPT
                     NANSIYIGDCYGNLIVHSSDQGLTWTPMDGNYHMDIHHLLYSPSGELWAGTDGGISKL
                     TTSGWINKTSNIGVANLHYIAGAATDSNLVLMGAYDIGCNMLDKKQQDIDRWTAYNIG
                     DGTSCAFDNHDKNRFYVAGQSGKYIKRTDDFGQHFTDISQFDETDFFTCLKLSPIVDS
                     TVYLVALFDVYRSRELGNDNTWHPITTGITMHDTLKHFYKLYTSPNNADVMYMRDLAT
                     KWEPQRWFKTTNANNSDPNTVTW"
BASE COUNT          542 a          355 c          315 g          565 t
ORIGIN      
        1 accatgtcac agtattggga tcggaattat tggcatttgt ggttttaaac catctttgag
       61 gctcccattt agtcgccaaa tctctcatgt acattacatc tgcattattt ggagaagtat
      121 aaagcttata aaaatgcttt aaagtatcat gcattgtaat tcctgtagtt attggatgcc
      181 acgtattatc attgcccagt tcacgtgaac ggtaaacatc aaataaagct acaagataaa
      241 ctgttgagtc aacaatgggg cttaatttca aacaggtaaa gaaatcagtt tcatcgaatt
      301 gagatatgtc agtaaaatgc tggccaaaat catctgttcg tttaatatat tttccggatt
      361 gtcctgcaac ataaaatctg tttttatcat gattatcaaa agcgcaactt gtgccatctc
      421 caatattata tgccgtccat ctatcaatat cttgttgctt tttgtccagc atattgcatc
      481 ctatatcata agcccccatt aataccaggt tagaatcagt tgctgcacct gcaatataat
      541 gcaaatttgc cacaccgata ttgctggttt tattgatcca gcccgatgtg gttagtttcg
      601 agatcccacc atctgttcct gcccataatt ctcctgaggg agaatataat aaatgatgaa
      661 tatccatatg gtagttacca tccatcggcg tccatgtaag accttgatca gaagaatgta
      721 caattaagtt cccataacag tctccaatat atattgagtt tgcattagtt ggtgaaacag
      781 caaacccttt ggcaatatta tataagtagt tagtatagtt gttctcaaga tattttaccc
      841 atccccttga tatccattgg ttcgtgtaaa gattcagtat atgaagttgt attgtgtcgt
      901 agcattctgt aataaataga agatcagggt ttgcaggcga aaactcaatc ataatattgg
      961 tggtgtctcg aaatggagta tttggaataa gactgaatga atcaccgcca tttgtggaaa
     1021 ccaacaactt gcttccataa taccctgaag catacatagt atcattattg ccaggcctat
     1081 aatggaggtc gttaaatgat cctgaacata ctctattcca gattgggtta gcagcggttg
     1141 cattggtagt cttataaata gccgtatcgg ttactgtaaa caatatgttg ttatttgtag
     1201 ggtgcataag gagttttcgc acacggttag gcctgtaaaa caatgaagta tcaaaaattt
     1261 gtacccatgt gactcccata tcttctgtgc ggtaaacgcc atcggatgta ttcatacacc
     1321 agttaactga cgcgccgtca caatcacctg tacctatata ccaacggcta ctgttttgcg
     1381 ggtcaacaac caaatatgaa acaccaacaa ttttcattga atcagtatgg ccgttattcc
     1441 aggtattacc accatctaag gaatagaaga ggcctccggt tggagacatt gcaaaaacat
     1501 gattcgcatt attaggatta aaagcaacag cgtgaatccg gccaatacct gcataatttt
     1561 tcaaactatc aatatttgtg ggaagaaatt taggtccaag ttctgtccag ttgccattaa
     1621 cacttggcat cctgttttta atatcattaa aactttcata aaaatctaga actttcgatt
     1681 gagcctcttt catactgcct gaaggtccaa taatatgact gaattcttct ttccatcgca
     1741 tataacccga atatcctgta cccttcatat tctctac
//
LOCUS       DAOPYB010000371         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_101592_length_1775_cov_8.313953, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000371 DAOPYB010000000
VERSION     DAOPYB010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101592_length_1775_cov_8.313953"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..>1775)
                     /locus_tag="PKH58_14090"
     CDS             complement(124..>1775)
                     /locus_tag="PKH58_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90202.1"
                     /translation="IQLRGNLYYINDIQREHSSLQRTIYTPVDTLQFTEQLNNHHFDN
                     YLQGEFTLSRNVKSNYLNNKLKIQSGWDRQSGLAYTGDENINQSLKKPFQSISNELRS
                     INPVGAYLVEFRSLIMYNYSPHCLTVEPGMFEDALNQGEIYDMAKQELNLQRFYTDNS
                     AGFTFGWKRLVFTPRVGIAYRQQTLESKILITQQGTERIAGSDFSNNLDGTQTRTYLQ
                     TDIECKLNKITFSAGLPLNWQHVNLNDTRLNQKQELTRLCFDPSLSVDYSITGFWRLR
                     SSWTLSNKLGDIDMIHYGFILGNYRDLSLQAAPVSKITSNNFSLSLSYRNPIISFFNS
                     LNYIYSISNNNLTYSSIVQNDGTTIVKAIYLPQTAYTHYLTGYTSKFFTAIKTTLSLR
                     ANYNQRQGKTLMNDELLNTKTLFYNFKPDINIRLFQWLTAEYNLNSSYIETFIDNERK
                     SNISMLSHNLIFFVSPNDNHLVSLTSEYYDHEGNTTFFGDVSYRYTILKSKKKVDFEL
                     KWNNIFNNKTYTTYQASSFTVWKSTYDLRPSQVMLSAKFSF"
BASE COUNT          525 a          312 c          368 g          570 t
ORIGIN      
        1 ttaatcacaa aattgatcca tttttatctg ttaaacggca aaaaagatga ggcgtcgatc
       61 tgcgcagaca tcaagttctt tttatacggt ttggtttgga aaaattgaat ttctttagcc
      121 cggttaaaaa ctaaacttcg cagacaacat cacctgtgat ggacgtaaat cgtaggttga
      181 tttccaaacg gtgaatgaac ttgcctgata ggtagtatat gttttgttat tgaaaatgtt
      241 gttccatttc aattcaaagt caactttttt ctttgatttt aaaatggtgt agcgataact
      301 tacgtcgcca aagaaagtag tgtttccttc gtgatcataa tattcggaag tcaggctgac
      361 caaatgatta tcattgggag aaacaaagaa aatgaggtta tggctgagca tggaaatgtt
      421 gctcttgcgt tcattatcaa taaaagtttc aatataactt gagtttaaat tatactcagc
      481 agtcagccac tggaaaaggc ggatattgat atccggttta aaattataaa acaacgtctt
      541 ggtatttaac aattcgtcgt tcatcaatgt ctttccctgg cgttgattgt agttggcgcg
      601 cagacttaat gtcgttttaa ttgccgtaaa aaatttactg gtatatcccg tcaggtaatg
      661 ggtataagcc gtttgcggca aataaatggc tttcactatg gttgttccgt cattctgaac
      721 aatactgctg tatgtaagat tgttgttgct aatggaataa atgtaattca acgaattgaa
      781 gaaggatatg atgggattac ggtacgacag agataatgaa aagttattgg atgtaatttt
      841 tgaaacgggc gctgcctgaa gactaagatc ccggtaattt cccaggataa acccgtagtg
      901 tatcatgtcg atgtcgccta acttgttgga aagggtccac gaacttctta accgccagaa
      961 accggtaatg gaataatcca ccgataaact gggatcaaag cataaacggg taagctcctg
     1021 cttttggttt aagcgtgtat cattcagatt aacatgttgc cagttgagag gcaatccggc
     1081 actaaaggta attttattaa gtttacattc aatgtcggtt tgcaaatagg tacgggtttg
     1141 agttccgtcc aggttattgg agaaatcact cccggctatc cgttctgtcc cctgctgtgt
     1201 gataagaatc ttactttcga gtgtttgctg ccggtaagct attcctaccc ggggagtaaa
     1261 aaccaatctt ttccaaccga atgtaaaacc ggccgagttg tcggtataaa aacgttgcag
     1321 atttaattcc tgttttgcca tatcataaat ttcaccctga ttcaatgcat cctcaaacat
     1381 tccgggttca accgttaaac aatgaggact gtagttatac ataatcaggg atcggaattc
     1441 caccaaatat gctcctacag ggtttatcga gcgtaattcg ttagaaatgg attgaaaagg
     1501 tttttttaac gattgattga tattttcatc tcccgtataa gccaaccctg attgcctgtc
     1561 ccagcccgat tgaatcttta atttattatt caggtaattg ctttttacat ttctgctaag
     1621 tgtgaattct ccctggagat aattatcgaa atgatgatta ttgagttgtt ctgtaaattg
     1681 caatgtatcc acaggagtat aaattgtacg ctgcagggaa gaatgctcac gctgaatatc
     1741 gttgatataa taaagattgc ctcttagctg aatgt
//
LOCUS       DAOPYB010000372         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_102781_length_1762_cov_9.079672, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000372 DAOPYB010000000
VERSION     DAOPYB010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102781_length_1762_cov_9.079672"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKH58_14095"
     CDS             <1..408
                     /locus_tag="PKH58_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90203.1"
                     /translation="EQMQNKIMICRTAPGGNYWYEMLPSHYVDPWAYPNPNWGGTPNY
                     YQYLLFHSYSANPNHHGCLTPEECNFYLAKAKWIIYNAESAGGERPDDASFVSVDVNG
                     NVIFDLNTSTYIHWMEIDYGILHVSPNPPVPID"
     gene            481..>1762
                     /locus_tag="PKH58_14100"
     CDS             481..>1762
                     /locus_tag="PKH58_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90204.1"
                     /translation="MKIILTSLSLVLLTSCLYSQPSFSLLINSPNRELAVGGIIEFSP
                     SRYILSGTQSIALGQKVWQKFYKIDGEGDTIQSLVIEKDTSCMFFELYQINEKDILCF
                     GAILNRLKPAEIWLTCIDTSLNLLWEKSIQTPIDQIYTIKASENHDSTLIIAAGLEVD
                     FYTRYILMLRINQDGDSLYSKYIGPNTAYIFDLMRTPKTNQLLMPVLGLSQQTNTPGQ
                     IIIFNSSFNICQVDSIPNYVGNCCTVKSLNDTAYFLAGNKYYAFSNESYNIALMKMIF
                     PNLEINSAIVGRSADTMDFAGTFQGMDFINPSNIYIAGTSNISFGGYYAYQPSWYLIS
                     RFDSTLNLYWTKYYGGDAYYVLQSVTATSDGGAIVAGTRFDYLNHPENQLDVYVLKID
                     STGLYTNVDKEEPVPVYDAIVYPNPGSDYLIVQSG"
BASE COUNT          553 a          350 c          320 g          539 t
ORIGIN      
        1 gaacaaatgc agaacaagat tatgatttgc agaacagccc ccggcggtaa ctactggtac
       61 gaaatgctcc cgtctcacta tgttgatcca tgggcttatc ccaatccaaa ctggggtgga
      121 acaccaaatt attaccaata cctattgttt catagttaca gtgcgaatcc caatcaccac
      181 ggttgtttaa ctcccgaaga atgtaatttc tatctggcaa aggccaagtg gattatctat
      241 aatgccgaaa gcgcaggtgg tgaaagacca gatgatgcaa gttttgtttc ggtggatgtg
      301 aatggtaatg tgatatttga tcttaatact tcaacttaca ttcattggat ggaaatagat
      361 tacggaattt tgcatgtttc acctaaccca cctgtaccta ttgattaaat taatttcaca
      421 accatatcag ggtattattt gccctgatat ggtttatttc agtctaaata acacgcgatt
      481 atgaaaatta ttctaacctc actatcactt gtactactca caagttgttt atacagccaa
      541 ccatctttca gtttattaat aaactccccc aacagggaat tggctgtggg cggaataata
      601 gagttctccc catcaagata tattctgagt gggacacaga gtatagcact tgggcagaaa
      661 gtatggcaaa agttctataa aatagatggc gagggtgata ctattcaatc tcttgttatt
      721 gaaaaagata catcctgtat gttttttgaa ctctatcaaa taaatgaaaa ggatatttta
      781 tgttttggtg ccatattgaa tcgattgaag ccagccgaaa tctggttaac ctgtatagat
      841 acttcattga atttactgtg ggaaaaatcc attcaaacac caatagatca aatttataca
      901 ataaaagcat cagagaatca tgattcaact ttaattattg ccgctggttt agaggttgac
      961 ttttacacaa gatatatttt aatgcttagg ataaaccaag acggggactc tttatattca
     1021 aaatatattg gtccaaatac agcctatata ttcgacctga tgagaacgcc taaaactaac
     1081 caattgctta tgcctgtttt gggtttatcc caacaaacta atacaccagg tcaaatcata
     1141 atttttaaca gctcttttaa catatgccag gtagattcca ttccgaatta tgttggaaac
     1201 tgttgtacag ttaaatcatt gaacgatacc gcgtattttc tggcaggaaa taaatattac
     1261 gcattcagta atgaaagtta taatatcgcc ttaatgaaaa tgatattccc aaatttagaa
     1321 ataaactctg ctatagtcgg gcggtctgcc gatacaatgg actttgccgg tacttttcaa
     1381 ggcatggatt tcatcaatcc ctcaaatata tatattgcag gaacatcaaa catttcattt
     1441 ggaggttatt atgcttatca accctcgtgg tacctcatca gccgcttcga ttctactttg
     1501 aacctttact ggacaaaata ttatggcgga gatgcctatt atgtactgca aagcgtaacc
     1561 gccacttccg acggaggagc tattgttgcc ggcacccgtt tcgattatct taatcacccg
     1621 gaaaatcagc tcgatgttta tgtgctcaaa atagactcaa ccgggctcta tactaatgtg
     1681 gataaggaag aacctgtacc cgtttacgat gccattgtat atcccaatcc cggcagcgat
     1741 tacctcatcg tacaatcggg gc
//
LOCUS       DAOPYB010000373         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_107817_length_1709_cov_6.953446, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000373 DAOPYB010000000
VERSION     DAOPYB010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107817_length_1709_cov_6.953446"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1291)
                     /locus_tag="PKH58_14105"
     CDS             complement(188..1291)
                     /locus_tag="PKH58_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNX90205.1"
                     /translation="MYLYDNQNWPVFEWNSEKLLPLLSYVRNKQGKLIGKMGSLGFEL
                     QNEANLEILTIEIVKSTEIEGEILDREQVRSSIARRLGLDISGLVYSERNVDGMVDLM
                     LDATKNYNKELTKERLFSWHASLFPAGQSGMYKIITGNWRDDSTGPMQVVSGPLGKEK
                     VHYQAPPAAQLENEMRIFFDWFNLEQRVDPVLKAAIAHLWFVTLHPFEDGNGRISRAL
                     SDMLLALSDEQSYRFYSMSTQIRKERNSYYDILEKTQKGNLDITNWLEWFLNSLLHSI
                     EGSEKLLEKVIYKHEFWIRHSKVTINDRQCKILNLLMDDFEGVLNTTKWAKIGKCSQD
                     TALRDIQDLVEKGMMTKAKQGGRSTNYELKTNE"
BASE COUNT          455 a          365 c          277 g          612 t
ORIGIN      
        1 agtcaacgga aaataccggg tggagaaaat actgacgttg gatgaagtag ggaaataacg
       61 gagtgatatt ctatttcaac caataatcac agcttataat aagacttttt tatactcctg
      121 aatgcaaatc cccgctggta cggattatta ttccgagcca gcggggatta tgttaccaga
      181 ttttggtcta ttcattcgtt ttcaattcat aattagtact tcgtcctcct tgtttagctt
      241 tcgtcatcat tcctttttca actaaatcct gaatgtcgcg taacgccgtg tcctgtgaac
      301 atttccctat ttttgcccac tttgttgtat ttagcactcc ttcaaagtca tccattaaca
      361 ggttgagaat tttacactgc ctgtcattga tagtcacttt tgagtgcctt atccagaatt
      421 cgtgtttgta aatgactttt tccaacagtt tctccgaacc ttcaatggaa tgcaataaac
      481 tattcagaaa ccattcaagc cagttagtta tgtctaaatt tcctttttga gttttttcca
      541 atatgtcata atacgaattt cgttctttgc gtatttgtgt cgacatacta taaaaccggt
      601 aagattgttc gtcggaaaga gccagtaaca tatcgcttag tgctcttgaa attcttccat
      661 ttccgtcttc aaacgggtgg agcgtcacaa accacaagtg tgctattgca gcttttagaa
      721 caggatcaac acgctgttcc agattaaacc aatcaaagaa aattcgcatt tcattttcaa
      781 gctgagctgc cggtggcgcc tgataatgca ctttctcttt tcctaaagga ccggaaacca
      841 cttgcatggg tccggttgaa tcgtcacgcc agtttcctgt aataatttta tacattccgc
      901 tttgaccggc cgggaaaagt gaagcgtgcc agctgaaaag tctttctttt gttagttctt
      961 tgttgtaatt tttagttgca tcgagcatca aatcaaccat gccgtccaca ttccgttccg
     1021 aataaaccaa ccctgagata tctaaaccta atcttcttgc aattgaagac cttacctgtt
     1081 ctctgtccag aatttcccct tcaatttccg ttgatttgac aatctcaatc gttaatatct
     1141 caagatttgc ttcattctga agttcaaagc ccaatgaacc catttttcca atgagttttc
     1201 cctgtttgtt tcgaacatac gataataatg gcaatagttt ctcactattc cattcaaaga
     1261 caggccagtt ttgattgtcg tataaataca tatttgttat tctccgctaa atttgcggct
     1321 aaaatactac ttattctccg catttcaaaa tattctccgc aaataatgcg gtgaatgtgt
     1381 cagttattct ccgcatttca aattttttcc tctacactaa cagattattg atatgaaacc
     1441 cttagagatt gctggttgct ttcctgttca gttacaccaa acctgatgcg gggcagaatg
     1501 ccttaataat cacttaaacc ctaattttat aaagcttttg tatgcgtttc ggtgtttggt
     1561 aatatttcaa ttcttcttaa tataatcgtg actctgattt tggattcaga acttattttt
     1621 cttcatctct tttttgtagt ttttaatcac ttttccaaac tctttatcct ttttccgttt
     1681 ctcttcaatg gttgattcaa aatgtgcat
//
LOCUS       DAOPYB010000374         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_112891_length_1660_cov_7.545171, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000374 DAOPYB010000000
VERSION     DAOPYB010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112891_length_1660_cov_7.545171"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PKH58_14110"
     CDS             <1..1268
                     /locus_tag="PKH58_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX90206.1"
                     /translation="RGIRVIVDYSWNHTGTNFWAWLDILKNQEKSKYKDWYEISSFDN
                     PDTPENEFAYNGWGGYGYLPELKKVNLTTPRIHGLPYEGNIQEGPKKHIFDVTRRWLA
                     PDGDVSKGIDGYRLDVAEQIGLGFWRDYRKYVKSINPDAYLVGEIWWQKWPDVLMNPA
                     PYMQGDVFDAVMFYQVYKPARSFFNNSQDEINARQLRDSLEYQWNNIPGKNEYAMMNV
                     AATHDAPRLLTDFYNKNRYKFKANPEENPAYKTGKPDNDAYQRTRLYLIHVFTTLGSP
                     QIWNGDEMGMWGADDPNCRKPLMWPELKMEKEYRNNFQPGEKSYDKIRFDKKLFEFYK
                     KIIRIRKDNPVLSLGEFSFTVNEGKKLGYTRSDGKNEIVVLFNLEDQQGDFKLPEGNF
                     TDLLSGKNYGNGNIRLKPMKAAILKKSDK"
BASE COUNT          552 a          309 c          339 g          460 t
ORIGIN      
        1 aacgcggtat ccgtgttatt gtggattatt cgtggaatca taccggaacc aacttttggg
       61 catggctgga tatactgaag aatcaggaaa aatcgaaata caaagactgg tacgaaatca
      121 gttcgttcga taatcccgat actcccgaaa acgagtttgc atacaatgga tggggtggct
      181 atggctatct gcctgaactg aagaaagtca atctgaccac tccccgtatt cacggattac
      241 cctatgaggg aaatattcaa gaaggtccaa agaaacatat tttcgatgta acacgccgct
      301 ggcttgcccc cgacggtgat gtatcgaaag gaattgacgg ttaccggctg gatgtggccg
      361 aacaaatcgg tcttggtttc tggcgcgatt atcgcaagta cgttaaaagc atcaatcccg
      421 atgcatattt ggtgggagaa atctggtggc aaaaatggcc ggatgttctg atgaacccgg
      481 ctccctatat gcagggtgat gtgtttgacg ccgtaatgtt ttatcaggtg tacaaaccgg
      541 caagaagctt cttcaacaat tctcaggatg aaattaatgc cagacagcta agagacagtc
      601 tggaatatca gtggaataat atccctggta aaaatgaata tgccatgatg aatgtggcag
      661 ctacccacga tgcaccacga ctcctaaccg acttctataa taaaaacagg tacaaattca
      721 aggcaaatcc cgaagaaaat cctgcttata aaacgggtaa accagataat gatgcatatc
      781 aacgaacccg gctttatctt atccatgttt ttacaacgct tggttcacca caaatatgga
      841 acggcgatga aatgggtatg tggggtgccg acgatccaaa ctgccgcaaa ccgcttatgt
      901 ggcctgaact gaaaatggaa aaagaatacc ggaataattt tcagccggga gagaaatctt
      961 atgataaaat ccgatttgac aagaaacttt ttgaattcta taaaaaaatc attcggatca
     1021 gaaaagataa ccctgtactt tctttaggtg aattcagttt caccgtgaat gagggaaaaa
     1081 aactgggtta tacacgttca gacggtaaga atgaaatcgt tgttttgttt aatctggaag
     1141 atcagcaagg tgatttcaag ttacccgaag gtaattttac agatttgctg agcggaaaaa
     1201 attacggaaa cggtaatatc cggttgaaac caatgaaagc tgcgatatta aaaaaatcag
     1261 ataaataagt ttatattaag atctttatat tcagtatagt gccggatgtt ggaattatcc
     1321 ggcactatta tttccgtata tattatatac aaacctaatc tgttaattta cagcgcaaca
     1381 catttctcaa acgtttgcac caattctaaa atcaaatcgg aagttaatac tcagtaagac
     1441 ttaaaatttg cactattttt gtaactgttt aacaaccatt taatcctaaa aaggaataaa
     1501 ctctgatatc ataatcctat aattatatta atcatgaaga agactactct cttaaccttg
     1561 ttttgtttcg tattttcact gatgagctgg gcgcagataa tcacgtcaaa cccggctttt
     1621 gtaaccaaag attataatgg tgtaattgaa ataacttatg
//
LOCUS       DAOPYB010000375         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_114505_length_1645_cov_11.833962, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000375 DAOPYB010000000
VERSION     DAOPYB010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114505_length_1645_cov_11.833962"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PKH58_14115"
     CDS             <1..1113
                     /locus_tag="PKH58_14115"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX90207.1"
                     /translation="MNVLLDYVAHHIHELHPLYKQHPEFFTSLYLPDGTLNTERWNDH
                     RLTTWFDTFMPTFDLANPKVISMMSDSAMYWLREFKLDGFRHDACKHITEEFWRALTL
                     KIKQNNNGKGVYQIGETYGSPELIGSYLNTGMLDGQFDFNVFDEASTTLEGVGVPNMQ
                     RIYNVLKTSFNTYGYHNLMGYISGNHDKPRFMAYASGDLKFGEDSKAAGWNREIGITD
                     STAYDKMALMQTFIMTIPGVPVIYYGDEIGMTGANDPDCRKMMRFEGLNNRETKLWNQ
                     VATLTHLRSENPVLIYGDFINLKAETESWAYARKYFDKEAIVLINNSKAEKKLEIELP
                     ASLKNTNLKATFKNKFKLSDGKLQITIPAYSAEVLI"
     gene            1236..>1645
                     /locus_tag="PKH58_14120"
     CDS             1236..>1645
                     /locus_tag="PKH58_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP19 family protein"
                     /protein_id="HNX90208.1"
                     /translation="MIQISDKTLTSAAEKGMDEFLNVFIDAYLAALGGNLTAENMHLL
                     NGTQHTLLAYHFLREEITQGGFVQLIQNGYGGYVFGNPTAKALKIFGAADMAKIIYKA
                     KEIYDANKEELERETTEEEFTAMYVDFEAFDDLE"
BASE COUNT          507 a          377 c          367 g          394 t
ORIGIN      
        1 atgaacgtgc tgcttgatta tgtggctcat catattcatg aactgcatcc gctttacaag
       61 caacatcccg aatttttcac atcgttgtac ctgcctgacg gcacactcaa caccgagcgc
      121 tggaacgacc accggctcac cacctggttc gatacgttta tgcccacatt tgatctggct
      181 aacccgaagg taatcagcat gatgtccgac tcggctatgt actggcttcg ggagttcaaa
      241 cttgacggct tccgccacga cgcctgcaaa cacatcactg aagaattctg gcgtgcgctg
      301 acattgaaaa tcaagcaaaa taacaacgga aaaggtgttt atcagattgg tgaaacttat
      361 ggaagccccg aactcatagg cagttacctg aataccggca tgctcgacgg acagtttgat
      421 ttcaacgtgt tcgacgaagc ctccaccacc ctcgaaggtg tgggagttcc caatatgcaa
      481 cggatttata acgtgttaaa aaccagcttt aatacctacg gatatcacaa cctgatgggg
      541 tatatctccg gcaaccacga caaaccccgc tttatggcct atgcttccgg tgatctgaaa
      601 tttggtgaag acagcaaggc ggccggctgg aaccgtgaaa ttggcattac cgacagcacc
      661 gcgtatgaca aaatggcgct gatgcagact ttcatcatga ccataccggg tgttccggtg
      721 atttactacg gtgatgaaat aggaatgacc ggtgcaaatg acccggattg ccgcaagatg
      781 atgcgtttcg aaggactgaa caaccgggaa accaaactct ggaaccaggt agcaacgctt
      841 acacacctga ggagcgaaaa tccggtactc atttacggtg atttcatcaa cctgaaagcc
      901 gaaaccgaaa gctgggctta tgcccgtaag tatttcgata aggaagctat tgtgttgatt
      961 aataattcga aagcagaaaa aaaactggaa atagaattac ccgcaagttt gaaaaatacg
     1021 aacctgaagg ctaccttcaa aaataaattc aaattgtcgg atggaaaatt gcagattaca
     1081 attcctgcat acagtgcgga agtgctgatc taaaaaatat cccgaaatcc ctcccgaagc
     1141 aagttcggga gaggactttt ggaaagttct aaaaaatctt tataaaaaaa taatttaata
     1201 ctaatttccc cctcatgggg gattaagggg gcctaatgat acaaatctca gacaaaactc
     1261 ttacttccgc tgccgaaaaa ggcatggatg aattccttaa cgtatttatt gatgcatatc
     1321 tcgctgcttt gggcggaaac ctcaccgctg aaaatatgca tctgcttaac ggcactcagc
     1381 acacgcttct tgcctaccat tttctgcgcg aagaaataac acagggcggt tttgtgcaac
     1441 tcatccaaaa cggctatgga ggctatgttt tcggaaatcc gacggcaaag gcgctgaaaa
     1501 tatttggtgc ggccgatatg gccaaaatta tttataaggc aaaggaaatt tacgatgcca
     1561 ataaagaaga actcgaacgc gaaaccaccg aagaagaatt tacggccatg tacgtcgatt
     1621 ttgaagcatt tgatgatctg gaaga
//
LOCUS       DAOPYB010000376         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_115308_length_1638_cov_7.732154, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000376 DAOPYB010000000
VERSION     DAOPYB010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115308_length_1638_cov_7.732154"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1297
                     /locus_tag="PKH58_14125"
     CDS             128..1297
                     /locus_tag="PKH58_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNX90209.1"
                     /translation="MNEGKYVFSQLVEFLPKRVFDCIVMRYQGDKYIKSFSCWNQLLI
                     MMYGQLAGCESLRELVCVTTAHTPKSYHLGFGKSVITRSNLAKANANRDCKVFEEFAY
                     KMISIAQGKRITKEFEIPGRFYAFDSTTIDLCLSLFWWASFRKTKGGIKVHTLFDVVT
                     QIPAFLHITAAKVHDMNAMDEIPYEQNAYYIFDRAYFDLARLFTINRIGSNFVIRERG
                     NLQYEIIEGEDLLESPDNILYDQTIRLTGQQTGKKYPSLLRRVGYFSVEHKRTFTYLT
                     NNFEISSKHVALLYKNRWQVELFYKWIKQHLHIKSFWGVTENAVRIQIYSAITAYCLV
                     AIVEHDLRLNRSTFDVLRILSMSLFDKTPIRELFERAEPMGDIVENGNVQLCFNF"
     gene            complement(1294..>1638)
                     /locus_tag="PKH58_14130"
     CDS             complement(1294..>1638)
                     /locus_tag="PKH58_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90210.1"
                     /translation="IVVAAFHQKGYDETSKQKIEKAKHKAATKILDDYDWDSKKDTMM
                     FQALRFFSNEATYPSRVNELVTEIINQFHQHKHERDSSLYFRRLNIIVGYPFHNIAIW
                     KGNCLYYITSVH"
BASE COUNT          476 a          301 c          326 g          535 t
ORIGIN      
        1 atcagtagtg tccactaaag ttttttaaaa tacgttataa cagtctattt ataagatata
       61 tacaacagtt tattcaacgt gtcgatttga atttgtaaat agtatccgat ttgctaaatc
      121 gggtataatg aacgaaggaa aatatgtttt cagccaactc gtagaatttc tgcctaaaag
      181 ggtttttgat tgtatcgtaa tgagatatca aggggacaaa tacattaagt cgttcagttg
      241 ctggaatcag cttctgatca tgatgtatgg tcagttggct gggtgtgaaa gccttagaga
      301 gctcgtctgt gttacaactg cacatacccc aaaatcttac catcttggat ttggcaaatc
      361 tgttataact cgcagcaatc ttgccaaggc taatgcaaac agggactgca aggtctttga
      421 ggaatttgcc tataaaatga tttctattgc ccaagggaag cgtattacca aggaatttga
      481 aattcccggt cggttctatg cctttgattc cacaacgatt gatctgtgcc tgagtctttt
      541 ctggtgggct agctttcgca agacgaaagg tgggatcaaa gtacataccc tttttgatgt
      601 cgtcacccag atcccggctt ttctccacat cacggcagct aaagtccatg atatgaatgc
      661 catggatgag attccgtatg aacagaatgc ttattacatt tttgataggg cttactttga
      721 tttggcacgc ctttttacta ttaacagaat tggatccaac tttgtaatcc gggagagagg
      781 taaccttcaa tatgaaatca ttgagggaga ggaccttctt gaaagtcctg acaacattct
      841 ctatgatcag acgatacgac ttaccggaca acaaactggt aaaaaatacc cgagtcttct
      901 tcgccgtgtg ggttactttt ctgtggaaca caaaaggact tttacctacc tgactaacaa
      961 ctttgaaatc agttctaaac atgttgcact gctgtacaag aacaggtggc aggtggaatt
     1021 gttctacaaa tggattaaac agcatctgca tattaaatcc ttctggggtg taactgagaa
     1081 tgctgtcaga atccagattt attcggcgat cacggcttat tgcctggttg ccattgtcga
     1141 acatgatctt cgacttaatc gcagcacttt tgacgttctc cgtatcttga gcatgtcgct
     1201 ttttgacaaa actccgatca gagaactctt tgaacgagcg gaaccaatgg gtgatattgt
     1261 tgaaaatgga aacgttcaat tgtgctttaa tttttagtgg acactagtga tataatataa
     1321 acaattgcct ttccatatag caatgttatg gaaaggatat ccgactatga tattcaatcg
     1381 tcgaaaatat aaagaactat ccctttcatg tttatgttga tgaaattgat ttattatttc
     1441 agttactaat tcgtttactc tactgggata agtagcttca tttgaaaaaa aacgaagggc
     1501 ttgaaacatc attgtatctt ttttactatc ccagtcataa tcatctaata ttttagtagc
     1561 tgctttgtgt ttggcttttt caattttttg ttttgatgtt tcatcgtaac ctttctgatg
     1621 aaaggcggca actactat
//
LOCUS       DAOPYB010000377         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_116687_length_1626_cov_12.537874, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000377 DAOPYB010000000
VERSION     DAOPYB010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116687_length_1626_cov_12.537874"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKH58_14135"
     CDS             <1..404
                     /locus_tag="PKH58_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90211.1"
                     /translation="LWLHIAAKYPIIHIPEYTNVYCLYEDSYTLGDVKRHEKELSFFR
                     YIFKKPTLRKLLPAASKNRLLSMCHYHLAVNANKNGQRMKMYAHTFRSFFLYPGGYNH
                     RTNKPLFVMCLYNFPVLGKLLQKVMEKKSTN"
     gene            486..1253
                     /locus_tag="PKH58_14140"
     CDS             486..1253
                     /locus_tag="PKH58_14140"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNX90212.1"
                     /translation="MKQLFRKIFSSKSVIGVFERLSGTKVLTNPPLGFSPILDLKYYI
                     NTHLWKKFIIFDVGANIGQTALVYRKEFPQAEIHSYEPVKSTFQELSVNTASKNIFCH
                     NLALGGISEYKKIHIYNSSDYNSFLLTEKDHASASTEMVTMLTFDDFCLDKNISAVNY
                     LKVDTEGYDLEVLKGATNSLTQHKIDFVETEISLNPTNTSHVDLHAMINYMNGFDYLL
                     FGIYEQHFDYVLKKPVLRRGNLVFISPVIYNDKTMYD"
BASE COUNT          516 a          345 c          286 g          479 t
ORIGIN      
        1 atctatggct tcatattgcc gctaagtatc cgattattca tattcctgaa tataccaatg
       61 tgtattgtct ttatgaagat tcatacacct tgggggatgt aaaaaggcat gaaaaggaat
      121 tgtcgttttt ccgctatatc ttcaaaaagc ccactctccg caaactacta ccagccgcat
      181 caaaaaaccg cctgctgagc atgtgccatt accaccttgc tgtgaacgct aacaaaaacg
      241 gccagcgcat gaaaatgtac gcgcatacat tccggtcttt tttcctctat cctggcggat
      301 acaaccacag gacaaacaaa ccgctttttg taatgtgtct ttacaatttt ccggtattgg
      361 ggaaactcct gcaaaaagta atggaaaaaa aatcaacaaa ttaatgacca ccactctaat
      421 caaataattt tccgtttata tggcccttaa gtaacatatc ctacttgtga aaacttacat
      481 cgcagatgaa acagctattc agaaaaattt tttcaagcaa aagcgtaatc ggagtttttg
      541 aaagactttc agggactaaa gttctgacca atcctccgct gggcttttca cccatactcg
      601 acctcaaata ttacatcaac acccatctgt ggaaaaaatt cataatattc gatgtgggag
      661 ccaatatagg ccaaacggct ttggtttatc gcaaggagtt tcctcaagca gaaatacatt
      721 cttatgaacc ggtaaaatcc acttttcaag agctgtcggt caataccgcc agcaaaaaca
      781 ttttttgtca taaccttgcc ctgggcggaa tttcggagta taaaaaaata cacatataca
      841 atagcagcga ttataacagt tttttgctga cggaaaaaga ccacgcttcc gccagcaccg
      901 aaatggttac tatgcttact ttcgatgatt tctgccttga taaaaacatt tccgccgtta
      961 attacctgaa agtggatacc gaaggctacg atctggaagt gttgaagggc gcaaccaact
     1021 cgctcacaca acataaaatt gattttgttg aaacagaaat cagccttaac ccgacaaaca
     1081 cctctcatgt tgacctgcat gctatgatca actatatgaa cggttttgac tatttgctat
     1141 ttggaatcta tgaacagcat tttgattatg tcctgaaaaa acctgttttg agacggggta
     1201 atctcgtatt tatttctcct gtgatttata acgacaaaac catgtatgac tgatgtatga
     1261 tcgctttggt cgaaaataat aacatacaaa ctatactata aaaatattta aaaatttatt
     1321 ttcattaaac agcaccatgg aatttttttg cagtagaaaa cattctcttt tcaaggcgga
     1381 tttgccccct tatattgagg ataaatacca tccgctgttt gccgcccggg cagaattttc
     1441 cgtaccggaa ttattcgtaa aagaatacaa gaatgtgttt gtgtcgcacg aaggactttg
     1501 cttacaaaat gggcggttgc tgccattttc gtcctttaat atttgcaccc gatacgacaa
     1561 ctcttttggg ataaaatatt acaaacttgt ttgggaacaa tacctggtca gtacttatgg
     1621 gaaaag
//
LOCUS       DAOPYB010000378         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_116814_length_1625_cov_10.544586, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000378 DAOPYB010000000
VERSION     DAOPYB010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116814_length_1625_cov_10.544586"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..1314)
                     /locus_tag="PKH58_14145"
     CDS             complement(295..1314)
                     /locus_tag="PKH58_14145"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNX90213.1"
                     /translation="MKKVFIGIDFSKLKFDAAIYSVDTKAIVSSEVFDNEQSGYVGFF
                     QWVKENTCCQKSEMLFCGEHTGYYSGNLSVFLYESGYDMWLVSGLEMKLTQGIKRNKT
                     DRVDACKIAEHAYRYQDKVVLFQPVSKALDEIRELLSYRERLVETRKVLTTSVNEMKR
                     VKDNTSCDYIYQDSIEYIKKLNASVRDCERRIETLMKQDVALKTNYKLVSSVVGIGLV
                     NAALIMVTTANFTLFDNPRKFGCYCGVVPFEHSSGSSVRGKTRVSKMANRRIKTTLTL
                     AARNSVRFDPELKEYYERKISEGKNSWLVMNNVKNKLIHRIFAVVRDGQMYEKQYIHP
                     LKQAA"
BASE COUNT          460 a          363 c          250 g          552 t
ORIGIN      
        1 gcgaatgcgg gcgcaaaacc cgctaagtgc acgtcagcca gcctgtgaac ataggaattc
       61 tatggttatt ccaaattatt tttgttaata attagttact ttacttgttt ttgtttctgt
      121 ttttgcgcac aaagctaaat ttcaaaaata acatgctcaa gaccgagcct acggtgatta
      181 aaaaaatctc cacacgaact tcgttcggta gtattttttc aatcagtctt gctcacgtta
      241 ttttcttcat ttattgtaag tttacaaaat caaaaacaaa gaaaaaaata atgattaggc
      301 tgcttgtttc aacggatgaa tgtactgttt ttcatacatt tgtccatctc tcacaactgc
      361 aaatatcctg tgtatcaatt tatttttcac attattcatc accaaccagc tgttctttcc
      421 ttcacttatc ttacgctcgt aatactcctt taactcagga tcgaaacgta cactattcct
      481 ggctgccagg gtcaatgtcg tttttatacg cctgttagcc attttactta cgcgcgtttt
      541 tccccttaca ctactgccag aactatgttc aaatggcacc accccgcaat aacaaccaaa
      601 cttccgggga ttgtcaaata gcgtgaaatt agccgttgta accattatta aagcagcatt
      661 taccagtccg ataccaacaa cagaactcac cagtttatag ttggttttca gcgcaacatc
      721 ttgcttcata agcgtttcga ttcgtctttc acaatcacgc acacttgcgt tcagcttttt
      781 tatatactcg atactatctt ggtaaatata atcgcacgag gtattatctt ttacccgttt
      841 catttcattg accgaagtgg ttagcacttt tcgtgtttct accaaccgtt cacggtagga
      901 aagcaattca cgtatttcgt ctaaggcttt tgaaacaggt tgaaaaagaa cgactttatc
      961 ctgataacga taagcatgtt cggctatctt gcacgcgtct acacggtctg tcttgttgcg
     1021 tttaatgccc tgagtaagtt tcatttccaa tcctgaaacc aaccacatat cgtaacctga
     1081 ttcatacaga aataccgata aattaccgga ataataacct gtatgctcac cacaaaacaa
     1141 catttcagac ttctggcaac aggtattttc tttcacccat tggaagaaac caacataacc
     1201 ggattgctca ttgtcaaaca cttcgctcga tacaatcgct tttgtatcaa cactgtaaat
     1261 tgctgcgtca aattttaatt tcgaaaaatc aattccaata aaaacctttt tcatactttt
     1321 gtgtactttt tatgggcttg gaattaaccg atagctgttc tgcactaaca ctcttattgg
     1381 tctctcaaat accagaattc tatttggtgt ctttcagcca gactatcagg ggtcttatac
     1441 ctatggtgaa ttatattcac gtgtgttact agtttaccct cgatagccgg ttaattcctc
     1501 gcttttttac aacaaaagta cagaaatcaa cctcgacctt caaaattcta attattgtgc
     1561 aaacctaaga gtgtgttagg ttctcgttca ttttttcatt gtcttgttgt tgtacttcaa
     1621 ctttt
//
LOCUS       DAOPYB010000379         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_117525_length_1619_cov_7.448210, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000379 DAOPYB010000000
VERSION     DAOPYB010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117525_length_1619_cov_7.448210"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..811)
                     /locus_tag="PKH58_14150"
     CDS             complement(221..811)
                     /locus_tag="PKH58_14150"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNX90214.1"
                     /translation="MIRFFDFLLSGIGLIVLSPIFLIFSIWIKLDSEGPVFYKQNRVG
                     KDNVEFLLYKFRSMVTDADKTGLLITVGGKDPRVTKSGYFIRKYKLDELPQLINVVWG
                     DMSLVGPRPEVRKYVELYNNEQRKVMTVKPGITDYASIEYIDENKILGESEDPEKTYI
                     EIILPEKIRHNMKYINNKSIKEYFKIILLTITKILQ"
     gene            complement(808..>1619)
                     /locus_tag="PKH58_14155"
     CDS             complement(808..>1619)
                     /locus_tag="PKH58_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNX90215.1"
                     /translation="IMSLVKEPEVKSQFVAETDNQQKLGRILVLNDAAHSLGAWYKKG
                     MRTGSETDIAIFSLHAVKNVTTAEGGAICLNLPEPFNNEALYSELHRMSLNCQTKDAL
                     SKSKAGGWRFDVVGFGMKINLADVNASIGLAQIRKYEKLLTERKRVFETYDKAFSKYE
                     WAILPQAEVGDKKTSYHIYALRIKDFTEELRDAMIEEITTKEVAVNVHFIPMPMLSFF
                     KQLGYNIEDYQVAYNNYKHEISLPIYPQLTEEEVDYVIESVIEAYEKVKPQ"
BASE COUNT          479 a          330 c          231 g          579 t
ORIGIN      
        1 ataaatcgtt ttattgattt atttttccct ctacccaacc gggtctgttt gaattcaagc
       61 tactgaagaa ttttcagaca atcaggggat aaattttcaa attgctacat tttcaaatta
      121 tcaaatcttc ttatcttcaa attttcaaat tgccacattt tcaaattatc atatcttcaa
      181 attatcaaat tttcaagacc cccgattgat aatataaaat tcattgaaga attttcgtaa
      241 tggtaagaag tattattttg aagtactctt taatcgattt attgttaata tatttcatat
      301 tatgtcgaat cttttcaggc aaaataatct cgatatatgt tttttcggga tcttccgatt
      361 ccccgagtat tttattttca tctatatatt cgatggatgc gtaatctgta attcctggtt
      421 tcacagtcat aaccttacgt tgctcattgt tatacaattc cacgtattta cgcacttcag
      481 gtctgggtcc aactaagctc atatcacccc aaacaacatt aattagttgt ggaagttcat
      541 ctaacttata tttccggata aagtaacctg atttagttac acggggatct ttacctccta
      601 ccgtaatcaa caatcccgtt ttatcagcat ccgtaaccat ggaacggaat ttatacaaca
      661 ggaattcaac attgtccttc cccactctgt tttgtttgta aaaaacaggt ccttcagaat
      721 ccaatttaat ccaaatacta aatattaaga atatcgggga taaaacaatc aatccgatac
      781 cactcagtaa gaaatcaaaa aagcggatca ttgaggtttt actttttcgt aagcttctat
      841 cacggattca ataacatagt ctacttcctc ttccgttaat tggggataga ttggtaagga
      901 tatttcatgc ttatagttat tataagctac ctgataatct tctatattat aacctagttg
      961 tttgaagaat gataacatgg gcatcggaat aaaatgaaca tttacggcta cctcttttgt
     1021 tgttatttcc tctatcatcg catcgcgcag ttcttcagtg aaatccttta tgcgtaatgc
     1081 ataaatatga taagatgttt ttttatctcc gacctctgcc tgtggtaata tagcccattc
     1141 atatttacta aatgccttgt cgtatgtctc gaaaactctt tttctttctg ttaacaattt
     1201 ctcgtatttc ctgatttggg caagtcctat tgaagcattc acatcagcaa gattgatttt
     1261 catcccaaag ccaacaacat caaatcgcca tccacctgct ttacttttag ataaggcatc
     1321 tttcgtctga cagttcaaac tcattcggtg taattcggaa tataaagctt cattattaaa
     1381 tggttcaggc aaattcaggc aaatagctcc accctcggct gttgttacat ttttaacagc
     1441 atgtagtgag aatatggcta tatctgtctc acttcctgtt ctcatccctt ttttatacca
     1501 tgcaccaagc gaatgagcgg catcattcaa taccagaata cgccctaact tctgctgatt
     1561 atcagtttca gctacaaact gagattttac ttccggttct tttaccaaac tcatgattg
//
LOCUS       DAOPYB010000380         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_121603_length_1584_cov_8.778286, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000380 DAOPYB010000000
VERSION     DAOPYB010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121603_length_1584_cov_8.778286"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..>1584)
                     /locus_tag="PKH58_14160"
     CDS             complement(269..>1584)
                     /locus_tag="PKH58_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HNX90216.1"
                     /translation="RMNDDNFCMDGMISPDQKYIPHTSIVKKVYQNIIFEAKDLKNGV
                     ITVINDFKFTGISTKNYTFKWVLLKNGENSAEGFFDVTAKADSRQDVKLKLPALEQKP
                     GVEYFLQLYAYTKQASELIPAGFEAAKGEMAFETNNYFVEENAEGSLVTEEKDNNLVV
                     KSGDVEYVFSLRDGQMLNNVKNEGSNVFYELPRLNFWRAPTDNDFGAGDQYKLRLWDA
                     ASHNLTSKYKGLEKQNSTVTVKYEVKPRGVEAKVDLIYTVNKDGSLTIKADYKALSDD
                     LPEMMRFGMLMTLPNDMDNFTWYGRGPGENYVDRNQDAFMGIWSGKVKDQAFEYFRPQ
                     ETGNKTDVRWLTLKTDNGQGIEIKGAQPLSVAAINYRPEDLDPGMTKKQQHWKDVKSR
                     WETVLYVDLFQRGVGGLNSWGAIPLNKYRFTAKEYQYSYTIKLLK"
BASE COUNT          404 a          337 c          295 g          548 t
ORIGIN      
        1 agagcatctg ccttacaagc agagggtcgg cggttcgaat ccgtcaacgc ccacgcaatt
       61 aagagttaca gagatgtagc tctttttatt tgatacctat ggggaatttt caataaaaat
      121 cgtacagaaa ttttcggcta ttgttgttta gtagttgatt gtatgagata attatattgc
      181 ctgatcagtt gtgcaagaag tatttaaaaa tagaaaagcc cttcacattt agcaaagggc
      241 tttatgatac tattttttaa ttatacggtt atttcaataa ttttatcgta tagctatatt
      301 ggtattcttt tgctgtaaaa cgatatttat tcaacggaat tgctccccag gagtttaatc
      361 cgccaacacc acgctggaat aaatcaacat aaagaaccgt ttcccaacgt gattttacat
      421 ctttccagtg ttgctgtttt tttgtcattc cggggtcgag atcttccggt ctgtaattta
      481 tagcagcgac agacagaggt tgagcgcctt ttatttcaat tccctgacca ttgtcggttt
      541 tcagcgttaa ccagcgtaca tccgttttgt tgcccgtttc ctgcggacgg aaatattcaa
      601 aagcctgatc tttcaccttt ccgctccaga tgcccataaa tgcatcctga ttccggtcga
      661 cataattttc tcctggtccg cgtccatacc aggtgaagtt atccatatcg ttgggtaacg
      721 tcatgagcat accaaaacgc atcatttcag gtaaatcatc tgaaagtgct ttgtagtcgg
      781 ctttaattgt cagactgccg tctttgttta ctgtataaat caaatcaact tttgcttcaa
      841 caccgcgggg ttttacttcg tattttacgg ttactgtgct gttttgtttc tccaatcctt
      901 tgtattttga ggtgagatta tgacttgcag catcccacaa cctcagtttg tactgatcgc
      961 ccgcgccaaa atcattatcg gtcggagccc gccagaaatt caagcgcggg agttcataaa
     1021 atacgttgct tccttcgttt ttgacgttat tcagcatctg tccgtctctg agcgaaaaca
     1081 catattccac atcacctgac tttacaacaa ggttattgtc tttctcttca gttaccaggc
     1141 ttccttctgc attttcttcc acgaaatagt tgttagtttc aaaagccatt tcacctttag
     1201 cagcttcaaa acctgccgga ataagttcag atgcctgttt tgtgtatgca tacagttgca
     1261 gaaagtattc aacgcccggt ttttgttcca atgccggaag tttcagtttc acatcctgac
     1321 gggagtcagc ttttgctgtt acgtcaaaaa atccttcagc tgaattctct ccatttttca
     1381 gcagtaccca tttgaacgta taattttttg ttgaaatacc ggtaaatttg aaatcgttga
     1441 ttacagttat cactccgttt tttaggtctt tagcttcaaa aatgatgttt tgatatactt
     1501 ttttcacaat ggatgtgtga ggaatatatt tttgatcggg acttatcatt ccgtccatgc
     1561 agaaattatc atcgttcatc cggt
//
LOCUS       DAOPYB010000381         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_123442_length_1569_cov_9.799868, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000381 DAOPYB010000000
VERSION     DAOPYB010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123442_length_1569_cov_9.799868"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKH58_14165"
     CDS             complement(<1..646)
                     /locus_tag="PKH58_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNX90217.1"
                     /translation="MRTIELLSPAKNLECGLAAINHGADAVYIGASLFSARAAAGNSI
                     EDIAGLVRYAHRYRSEVLVALNTVLTDEQLPDAQQLIRQLYEIGTDALIVQDMGILQL
                     DLPPIELHASTQTDNRTVEKVKFLQDVGFSRVVLARELTLNQIREISKQTEVELECFV
                     HGSLCVSYSGQCYISQAMTGRSANRGECAQYCRLPYQLTDANGEVLVKNKHLLSL"
     gene            complement(646..1482)
                     /locus_tag="PKH58_14170"
     CDS             complement(646..1482)
                     /locus_tag="PKH58_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90218.1"
                     /translation="MKYLNILIFLGIFYSLFSLILSIYNAISDNKDLKSQLNKKNYLY
                     LLTFVSLFFLDYLFFHSFNEALKASYAGLILTGVTIVIILTLKFGLNIWFKLSVNKLN
                     YLSSVDMSGFTIFKNDKQFSYSWMEFNDAVFDNTKQKLRLKGTRNIVIKKSLGNWYKL
                     LKTIPRTFPGLDYSYIDGLFNDLQTCMVCGMVAANALECMYCASDIWSDDLKSEFESE
                     EKYIIENQLDEFATMDKTEKFCNFILKDTFFNQDPNWKPLVTKEQVLEYSKNEYWDEK
                     QN"
     gene            complement(1490..>1569)
                     /locus_tag="PKH58_14175"
     CDS             complement(1490..>1569)
                     /locus_tag="PKH58_14175"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNX90219.1"
                     /translation="ANLLFTNWLNYFVYQETPYDLANIK"
BASE COUNT          481 a          317 c          288 g          483 t
ORIGIN      
        1 tgagtgaaag cagatgcttg ttttttacaa gcacttcgcc gttggcatcg gtgagctgat
       61 acggcagtcg gcagtattga gcgcattcac cgcggttggc actgcgtccg gtcatggcct
      121 ggctgatgta gcactgacca ctgtaactca cacacaagga accatgtaca aaacactcga
      181 gctccacttc tgtttgtttg ctaatttcac gtatctggtt cagtgtgagt tcacgagcca
      241 acacaacgcg cgaaaatccc acatcctgca gaaatttcac tttttccaca gtccggttat
      301 cggtttgcgt gctggcgtga agttctatgg gtggcaaatc gagttgcaaa atacccatat
      361 cctgcactat cagcgcatcg gtgccaattt catacagttg ccgtataagt tgctgtgcat
      421 ccgggagttg ttcatcggtc aaaactgtat tcaacgccac cagcacttca gagcgatatc
      481 gatgagcata acgaaccaat ccggcaatat cttcgatgga atttccggct gcagcccggg
      541 cgctaaacaa cgaagcaccg atatatactg catcggcacc gtgattgatg gctgccagac
      601 cacattccag gtttttggcc ggtgaaagaa gttctatggt tcgcattaat tttgtttttc
      661 gtcccaatat tcatttttac tgtattccag cacctgttct ttggttacaa gaggtttcca
      721 gttgggatct tgattaaaaa aagtatcttt cagaatgaaa ttacagaact tttctgtttt
      781 atccattgtg gcaaattcat caagctgatt ttcaatgatg tatttttctt ctgattcgaa
      841 ttcagatttc aaatcgtcgc tccagatatc agaagcacaa tacatacact ccagggcgtt
      901 tgctgcaacc attccgcata ccatgcacgt ttgtaaatca ttgaacaatc cgtcaatgta
      961 actatagtca agacccggaa atgttcgagg aatagttttc agtaatttat accaatttcc
     1021 caaggatttc tttataacaa tatttctagt accttttaat ctaagctttt gctttgtgtt
     1081 atcaaaaacc gcatcattaa actccatcca gctgtatgaa aattgtttat catttttgaa
     1141 aatggtaaat ccgctcatat cgactgatga aagataatta agtttattaa cagatagttt
     1201 aaaccagata ttcagaccaa attttaatgt taatattatg acaattgtaa ctccggttaa
     1261 aatcaatcca gcataagaag ctttcaatgc ctcattaaac gaatgaaaaa ataaataatc
     1321 taaaaaaaat aaagatacaa atgtcagcag atataaataa ttctttttat tcagttgaga
     1381 ttttaaatct ttattatctg aaatagcatt gtaaattgac aatattaggc taaataacga
     1441 gtaaaagatg ccaagaaaaa tcaggatatt caaatacttc atggcaacat tatttgatat
     1501 tagccaaatc gtatggagtt tcctggtata caaagtaatt cagccagttg gtaaatagca
     1561 gattagcgt
//
LOCUS       DAOPYB010000382         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_125544_length_1552_cov_9.843687, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000382 DAOPYB010000000
VERSION     DAOPYB010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125544_length_1552_cov_9.843687"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1481)
                     /locus_tag="PKH58_14180"
     CDS             complement(264..1481)
                     /locus_tag="PKH58_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNX90220.1"
                     /translation="MARKENSKLREMIKEYGIKDMNDVHEFVKMLTAETIQAALDAEL
                     ENELGYSKYDYKNKQTENSRNGYSTKTVQGSMGEVELQIPRDRQGEFEPQIVKKHQTD
                     VSGIEDKIIFLYSQGVSTRDIQKTMREMYGIDVDDSRVSKITDKILPLINEWQERPLQ
                     SVYAMVILDAVHYNVRESGIVTKKAAYVAIGTDLEGRKDVLGIWLGTSESSKYWLSVL
                     NGLKSRGVQDILIASIDGLSGFVEAINAAFPKAEVQRCIIHQIRSSTRYISYKDLKHF
                     TSDLKPIYKASTEEIALSSLKLLEEQWGKKYPLAVKSWYANWNELSTMFKYPPEIRTL
                     IYTTNAIENFNRQLRKVTKTKSAFVSDEALMKILYIATMSIVEKWTMPIKNWGSILDN
                     LMIYFGDRVKVQF"
BASE COUNT          416 a          334 c          271 g          531 t
ORIGIN      
        1 tttgtaactg ctgacaagtt ctgttgtcct gttgccgtcc cgttttggta tacacatttt
       61 gttttattaa agtcacattt ttttgcggtt tataatcaaa aataatatac aaacaattgc
      121 aataaaatta gtaagtaaag gtaagtatct aaacagacca atatctattg tgctaataga
      181 acaaaaaatg ccgatagcta tagtggcaat gaggtagcgc gtaaaatttt gtgtaaactc
      241 caaatatcac agtaatcaaa aagctaaaat tgaaccttaa cacgatctcc aaaatatatc
      301 ataaggttat ctaaaatact accccagttt ttaatcggca tcgtccattt ttcaacaatg
      361 ctcattgttg ctatataaag aattttcata agagcctcgt cagagacaaa ggcgcttttg
      421 gtttttgtta cttttctcag ttgtcggttg aagttttcaa tcgcgtttgt tgtgtaaatc
      481 agcgttctaa tttccggtgg gtacttgaac atcgttgaaa gttcgttcca attagcatac
      541 caggatttca ccgccagagg gtactttttc ccccactgtt cttcaagtaa ttttaaggaa
      601 gaaagggcga tttcttccgt tgaagctttg taaatcggtt tcaagtctga ggtgaagtgt
      661 ttaaggtctt tgtatgaaat gtaccgtgtt gatgaccgga tttgatgaat aatgcacctc
      721 tgaacctctg ctttcgggaa agctgcatta attgcttcta caaatcctga caaaccatca
      781 atcgatgcaa tcagtatatc ttgcacacct cggcttttca atccattgag taccgaaagc
      841 cagtatttgg aggactcgct cgtgccgagc catatcccaa gaacatcctt tctgccttcc
      901 aaatcggttc cgatagccac ataagcggct tttttagtta caatgccact ttccctgaca
      961 ttgtaatgca cagcgtctaa aataaccata gcatatacgc tctgcaaagg cctctcctgc
     1021 cattcgttaa tcaacggcag tatcttgtcg gttattttgg aaacacgact gtcgtcaaca
     1081 tcaattccgt acatttcccg catggttttc tgaatatcac gggttgaaac accttgtgaa
     1141 tacaaaaaga ttattttatc ctctatccct gacacatccg tttggtgttt ctttactatc
     1201 tgcggttcga attcaccttg ccggtcacgc ggaatttgaa gttcaacttc tcccatactc
     1261 ccttgtaccg tttttgtaga ataaccgttt cggctgtttt cagtttgctt attcttgtaa
     1321 tcgtacttac tgtagcctag ttcattctcc agttcggcgt cgagcgctgc ctgaatggtt
     1381 tctgccgtta gcatcttaac aaactcatga acatcattca tgtctttgat gccatattcc
     1441 ttgatcattt cccgtagttt actgttttct ttccttgcca tatgttttta cctccatcat
     1501 cgtttttatt ttacctgata atggaggttt acacaaatta ttagatacta cc
//
LOCUS       DAOPYB010000383         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_128048_length_1532_cov_9.829384, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000383 DAOPYB010000000
VERSION     DAOPYB010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128048_length_1532_cov_9.829384"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..714)
                     /locus_tag="PKH58_14185"
     CDS             complement(337..714)
                     /locus_tag="PKH58_14185"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX90221.1"
                     /translation="MKSIRKYQIIRAGFIAFLLFAATQVFATEYNVNGCVINTQDEPI
                     KNAVVTITDAKTHTPVATALCNAKGNFSLSDVPKGEYIITVYKGRVSRAKAKNLKINS
                     DGSIMYNNIENAQNSENDLLAIK"
     gene            complement(966..1463)
                     /locus_tag="PKH58_14190"
     CDS             complement(966..1463)
                     /locus_tag="PKH58_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90222.1"
                     /translation="METHQTRENTEPECCPKFEPELWDEKILEWDNKKFIKGSICTFM
                     YMPLNFGAVMRKLDAKIRNSGASMQKDGLCLSDHTSGWNMDIFLAVDREVEGLNNVTI
                     SGKYLCKVYEGDFRETGKWCKDYDSFAKSKNMKIEKMYMWYTTCPKCAKKYGKNFVAI
                     IGKIS"
BASE COUNT          453 a          261 c          293 g          525 t
ORIGIN      
        1 agtgagcata acggcgaaag ctatcaaaga aaaaaatact tttttcataa cgaatgacct
       61 taaaattaat actatatttg aataaagtga attaataaac agaagcaaat ttgctattta
      121 aactacaaat atataatatt aattgtgttt atgggcgaat ttaacactgt gtaattgaat
      181 tattgggaaa attaatagaa taggtcaggc tgaaaaaaca gatttccggt taccgaaaaa
      241 aaggtttacg ttgcctggaa ccgtaaacct ttgagtatag aagaataaga ttgtgaaagc
      301 ggggaaaatt tattaaatcc gtatgtagga tatccgttac ttgatggcta acagatcgtt
      361 ttcggagttt tgggcgtttt caatgttgtt gtacataatg gaaccgtcgc tgttaatctt
      421 caggttcttt gcttttgctc tcgatactct tcccttgtaa acggtaataa tatattcacc
      481 tttaggcaca tccgacagac tgaaatttcc tttagcatta cacagagcag tggcaaccgg
      541 agtatgtgtt ttggcatctg ttattgttac aacagcattt ttaatgggtt cgtcctgggt
      601 gttaatcaca caaccgttaa cgttgtattc ggtagcaaac acctgtgtgg cggcaaacaa
      661 caggaaagcg ataaatcctg cacgtataat ctgatatttt ctgatagatt tcatgatcgt
      721 tgaatattaa ttttatgagt taatttgaaa atgaattcag cgtttttttc cggagtgaag
      781 agttgactag tatttacttc tttcaggaaa taaaaccttg aattactgcg tttattaata
      841 agacgtttca aaaatagaat ttggattttt aagtactcat tattaaacac ttttaactca
      901 tgcagtcata aaaaaagccg gagggtttat gtccggcatc atgaaaaagc ttatgtgtgc
      961 ttgtttcagc tgattttacc aataatagcc acaaagtttt taccgtattt tttggcgcat
     1021 ttggggcagg tggtgtacca catatacatt ttttcgattt tcatgttctt gcttttggca
     1081 aaactgtcat aatctttaca ccatttcccg gtttcacgaa agtctccttc atataccttg
     1141 caaaggtatt tccccgagat ggtgacgtta ttcagtcctt ccacttcgcg atccaccgcc
     1201 agaaagatgt ccatgttcca gcccgaagtg tggtcggaga ggcataagcc gtccttttgc
     1261 attgaggcgc ctgagttgcg gatttttgca tccagtttac gcataactgc gccaaaattc
     1321 aaaggcatgt acataaaggt gcagatactt cccttgataa atttcttgtt gtcccattcc
     1381 agaatttttt catcccagag ttctggttca aatttcggac agcattcagg ttctgtgttt
     1441 tccctggttt gatgagtttc cataattacg ttgtaattta gtgagttaat ctacaaatgt
     1501 aaaaatttat tttgtatttt gtgttttaat aa
//
LOCUS       DAOPYB010000384         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_129695_length_1520_cov_5.406826, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000384 DAOPYB010000000
VERSION     DAOPYB010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129695_length_1520_cov_5.406826"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PKH58_14195"
     CDS             <1..1046
                     /locus_tag="PKH58_14195"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNX90223.1"
                     /translation="IFALLMISAACREEYYPSIDAGENALVVYGLVTNAHVPYAIKLS
                     RAVPYNSNDVQPAVSGALVYVVEGDKQYLFHETVTGKYTSDTTKFIALSGHRYTLHIE
                     TPDGEIYESSSEILPSPPRLDSIYGSLSDKEYLYRDVHGQLTTHLETGVETLINFTDT
                     SATTHQLRFDPTLLIEYTYIQQKSTSFPLIVYMWKKVNPNNIVSLTGSDDNARTTSNF
                     PVCFFPLEKYLYDIQSFQSIENWILGIKIYTLNDESLDYYQGIVEQTSATGKLFDPIT
                     SQLRGNIRCISHPDKLVLGLFETSSYNEITYHIRPGQANNNYFYQLTESIDGIPSSGK
                     SVQERPDFWHINK"
     gene            1051..>1520
                     /locus_tag="PKH58_14200"
     CDS             1051..>1520
                     /locus_tag="PKH58_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX90224.1"
                     /translation="MKHSAKVIIFSGILAIFLSGMAMAQTNLHSEKVFAHTDREVYVS
                     GELLFFKLYIVDAAIRQPSAISKTVYVVLRNTSNRSITEMSFQAEHSVAYGSILLPDT
                     LSSGVYQLVAFTHWMRNFGESSFFTKEIFVANALDRNLPVLNSLTERKILEQDS"
BASE COUNT          449 a          324 c          291 g          456 t
ORIGIN      
        1 ttatatttgc attactcatg attagtgcag cttgtagaga agaatattac ccttcaatag
       61 atgcgggaga aaacgccctt gtggtatatg gtttggtaac caatgctcat gtaccatatg
      121 ctatcaaatt gagcagagcc gttccttata actccaacga tgtacaacct gctgtttcgg
      181 gagctttagt ttacgtagtg gaaggagaca aacaatattt gtttcacgaa acagttaccg
      241 gaaagtatac atcggatacc acaaaattta ttgccttatc cggacatcgt tataccctgc
      301 acatcgaaac acctgatgga gaaatttatg aatcttcctc cgaaattttg ccatctcctc
      361 ccaggctcga tagtatttac ggaagtcttt ccgataagga atacctttac agggatgtgc
      421 atggacagtt aaccacccat ttggaaaccg gagtggaaac ccttattaac tttactgaca
      481 cttctgccac tacccatcaa ctccgttttg atcctacttt gttgattgaa tatacttata
      541 ttcagcaaaa aagcacatct tttcccttaa tcgtttatat gtggaaaaaa gttaatccaa
      601 acaatatcgt tagcctcact ggtagtgatg acaatgcaag aactaccagc aatttcccgg
      661 tatgcttttt ccctcttgag aaatatttat atgatataca aagctttcag agcattgaaa
      721 actggatttt agggattaaa atatataccc tcaatgatga atctctggat tattaccagg
      781 gaattgtgga gcagacgtcg gcaacaggca aacttttcga tcccatcacc tcgcagctta
      841 gaggaaacat ccgttgcata agtcatcctg acaagttagt acttgggctg ttcgaaacat
      901 catcttacaa tgaaataacc taccatatca gaccaggtca ggcaaacaac aattattttt
      961 atcagcttac tgaaagtatt gacggaatac cctcctctgg taaaagtgtt caggaaagac
     1021 ctgatttttg gcatataaat aaataacgat gtgaaacatt cagctaaagt catcattttt
     1081 tccggcattc tggccatttt tctttctggc atggctatgg cacaaactaa tctccatagc
     1141 gaaaaagtat ttgcacatac cgacagggag gtttatgtca gtggcgagct tctgtttttc
     1201 aaactttata ttgttgatgc tgctatacgt cagccatccg cgatcagtaa aacggtgtat
     1261 gtggtgcttc gcaacacgtc aaatcgttca attaccgaaa tgagttttca ggcagagcat
     1321 tccgtggcct atggcagcat tcttttgccc gacacgctta gctccggcgt ctatcaattg
     1381 gttgctttta cccattggat gcggaacttt ggtgaatctt cctttttcac aaaagaaatt
     1441 ttcgttgcaa acgccctcga caggaatttg ccggtactga attctttgac cgaacgaaaa
     1501 atactggagc aggatagtag
//
LOCUS       DAOPYB010000385         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1325
            NODE_131845_length_1505_cov_14.622759, whole genome shotgun
            sequence.
ACCESSION   DAOPYB010000385 DAOPYB010000000
VERSION     DAOPYB010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854530
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:51:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,841
            CDSs (total)                      :: 2,805
            Genes (coding)                    :: 2,791
            CDSs (with protein)               :: 2,791
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131845_length_1505_cov_14.622759"
                     /isolate="AMR_MDS_1325"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1459
                     /locus_tag="PKH58_14205"
     CDS             203..1459
                     /locus_tag="PKH58_14205"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HNX90225.1"
                     /translation="MIVNSRVHPQKRKGIDENLPLNLHVFFDKKRVITFNTGFRCDGN
                     QWDGKNQKMKINQVNKSGQSSSYVNVQLKAIRGAVDTWAKDNPTGTKDDLLKVLRTVA
                     GKKEKVEVQSDAEPETFFTLFDKFLKERKLSEGRYKHYMVLKRCLERYGMHTKQPVTF
                     EINVQSFEHYLTIEHTLFEKQDEKGKNTIIGLLKKLRSFIIWANSNEHTTINPFKTFK
                     IEEAKYGTPFFLTIAERNHLYNFNLSHRKDLAVQRDIFVFQCFVGCRVADLMRLTPAN
                     VIGNFLEYVATKTKDEQPTTIRVPLSATAKEIIKRYAGSEKLFPFTNPTIYNSDIREF
                     FTIAGLKRMVTVLDTKTRKDVQKPLNKVASSHIARRTFVGNLYAKVQDPNIVGSLSGH
                     VEGSRAFARYRKIDDEIKTDLIKSIE"
BASE COUNT          565 a          258 c          288 g          394 t
ORIGIN      
        1 gtgaccccga cagggtcata atttattaaa tcagattagt tcactcattt tatcgcagat
       61 ttataatgtg cttatattca ataaataata ctttgcaaag ataaacaaaa taaatcgaat
      121 ataattaggt tttatcaaaa aatgtgtgta attttgtatt tacacacatg gattacacac
      181 acgaattaaa acctaaaatg atatgatcgt aaatagtaga gttcacccgc aaaaaagaaa
      241 aggtattgat gaaaatttac ctttgaactt gcacgtattt tttgacaaaa aacgtgttat
      301 tactttcaac accggattta gatgtgatgg caatcagtgg gatggtaaaa accaaaaaat
      361 gaaaatcaat caggtgaaca agtcagggca atcttcaagt tatgtgaatg ttcaactgaa
      421 agcaatcagg ggagcggtgg acacgtgggc aaaggacaac ccgaccggca caaaggatga
      481 cttattaaaa gtacttcgaa cggttgcagg gaaaaaagaa aaagttgaag ttcaatcgga
      541 tgccgagccg gaaacatttt tcaccttatt tgataaattt ctgaaagaac ggaaactgtc
      601 tgaaggcaga tacaaacact acatggtatt gaaaagatgt ttggaacgtt acgggatgca
      661 caccaaacag cccgttacat tcgaaattaa tgttcaatca ttcgaacatt atctgactat
      721 tgaacacacc ttatttgaaa aacaagacga aaagggaaaa aacacaatta tcggactgct
      781 taagaaatta cgaagtttta tcatttgggc aaacagcaac gaacatacaa caatcaatcc
      841 gtttaaaact ttcaaaattg aagaagctaa atacggcaca ccgttttttc taactattgc
      901 cgaaagaaac cacttgtata acttcaattt gagccaccgg aaagaccttg cagtgcaacg
      961 ggatattttt gtatttcagt gtttcgtcgg ttgcagggtt gcggatttaa tgagattaac
     1021 accggcaaat gtgataggaa atttccttga atatgtagca accaaaacaa aggatgagca
     1081 accgactaca atacgtgttc cactgtcagc aacggcaaag gaaattataa aaaggtatgc
     1141 aggcagtgaa aagttatttc cgttcaccaa tccaacaatt tacaatagtg atatcaggga
     1201 attttttact attgccggac ttaaaagaat ggttacagta ttagatacaa agacccgaaa
     1261 agacgtacaa aaaccactta acaaggttgc aagttcacat attgcccgac gtacatttgt
     1321 gggcaatctg tatgcaaaag tgcaagaccc gaacattgtt ggaagtttat ccggacacgt
     1381 ggaaggatcg agagcttttg cacgttacag aaaaatagac gatgaaatta aaaccgacct
     1441 tattaaatca atagaatgaa ctacttaata aatacacgtg aattacagta ctgcaaaatg
     1501 gcgga
//
